LOCUS       DAKBNN010000001       105136 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000001 DAKBNN010000000
VERSION     DAKBNN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 105136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.001"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            83..1984
                     /gene="cooS"
                     /locus_tag="OUB02_000001"
     CDS             83..1984
                     /gene="cooS"
                     /locus_tag="OUB02_000001"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HCT8883819.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            1977..2450
                     /locus_tag="OUB02_000002"
     CDS             1977..2450
                     /locus_tag="OUB02_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCT8883820.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRS"
     gene            2425..3651
                     /locus_tag="OUB02_000003"
     CDS             2425..3651
                     /locus_tag="OUB02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT8883821.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(3790..4977)
                     /locus_tag="OUB02_000004"
     CDS             complement(3790..4977)
                     /locus_tag="OUB02_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8883822.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNTRIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            5252..6076
                     /locus_tag="OUB02_000005"
     CDS             5252..6076
                     /locus_tag="OUB02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8883823.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHREEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            6205..7470
                     /gene="gluD"
                     /locus_tag="OUB02_000006"
     CDS             6205..7470
                     /gene="gluD"
                     /locus_tag="OUB02_000006"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HCT8883824.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            7683..7796
                     /locus_tag="OUB02_000007"
     CDS             7683..7796
                     /locus_tag="OUB02_000007"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883825.1"
                     /translation="MHFKSKDFHLKYLVLFLKIVYWFNPFIYNGKIYGFGL"
     gene            7780..8271
                     /locus_tag="OUB02_000008"
     CDS             7780..8271
                     /locus_tag="OUB02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HCT8883826.1"
                     /translation="MDLDCEFYCDERVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFV
                     AGLNKQSDIRKRVINMFSEKYKNGILMALIISLFSSITFFKFDSTSIIEENDFLERSR
                     YTFEGRQEDVFVTIDFIYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMD
                     LKK"
     gene            8601..9740
                     /locus_tag="OUB02_000009"
     CDS             8601..9740
                     /locus_tag="OUB02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HCT8883827.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSDEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKYDMEKLHVFRGSVTLLEF
                     TYADAPENVKKEHKRMYEAMGLIPKDSDKISFGVKYYDKLNNLLR"
     gene            10211..11233
                     /locus_tag="OUB02_000010"
     CDS             10211..11233
                     /locus_tag="OUB02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT8883828.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            11529..12449
                     /gene="pyrB"
                     /locus_tag="OUB02_000011"
     CDS             11529..12449
                     /gene="pyrB"
                     /locus_tag="OUB02_000011"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCT8883829.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            12488..13180
                     /locus_tag="OUB02_000012"
     CDS             12488..13180
                     /locus_tag="OUB02_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCT8883830.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            13173..14075
                     /locus_tag="OUB02_000013"
     CDS             13173..14075
                     /locus_tag="OUB02_000013"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCT8883831.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            14160..14744
                     /locus_tag="OUB02_000014"
     CDS             14160..14744
                     /locus_tag="OUB02_000014"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCT8883832.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            14959..15885
                     /locus_tag="OUB02_000015"
     CDS             14959..15885
                     /locus_tag="OUB02_000015"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCT8883833.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(15937..16827)
                     /locus_tag="OUB02_000016"
     CDS             complement(15937..16827)
                     /locus_tag="OUB02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8883834.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            17015..18046
                     /locus_tag="OUB02_000017"
     CDS             17015..18046
                     /locus_tag="OUB02_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCT8883835.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            18340..19215
                     /locus_tag="OUB02_000018"
     CDS             18340..19215
                     /locus_tag="OUB02_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HCT8883836.1"
                     /translation="MNVYEKGNGVILERVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            19233..20723
                     /gene="cls"
                     /locus_tag="OUB02_000019"
     CDS             19233..20723
                     /gene="cls"
                     /locus_tag="OUB02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCT8883837.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            20916..21200
                     /locus_tag="OUB02_000020"
     CDS             20916..21200
                     /locus_tag="OUB02_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCT8883838.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            21233..22861
                     /gene="groL"
                     /locus_tag="OUB02_000021"
     CDS             21233..22861
                     /gene="groL"
                     /locus_tag="OUB02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCT8883839.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            23123..23863
                     /gene="larB"
                     /locus_tag="OUB02_000022"
     CDS             23123..23863
                     /gene="larB"
                     /locus_tag="OUB02_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HCT8883840.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEVSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            23947..24681
                     /gene="larC"
                     /locus_tag="OUB02_000023"
     CDS             23947..24681
                     /gene="larC"
                     /locus_tag="OUB02_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HCT8883841.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKFKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            24711..25130
                     /locus_tag="OUB02_000024"
     CDS             24711..25130
                     /locus_tag="OUB02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HCT8883842.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      25190..25291
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            25512..27047
                     /gene="guaA"
                     /locus_tag="OUB02_000025"
     CDS             25512..27047
                     /gene="guaA"
                     /locus_tag="OUB02_000025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCT8883843.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            27586..29016
                     /locus_tag="OUB02_000026"
     CDS             27586..29016
                     /locus_tag="OUB02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8883844.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(29164..30039)
                     /locus_tag="OUB02_000027"
     CDS             complement(29164..30039)
                     /locus_tag="OUB02_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8883845.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISHLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSKQALSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLKIILDNISNVL"
     gene            30159..30722
                     /locus_tag="OUB02_000028"
     CDS             30159..30722
                     /locus_tag="OUB02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HCT8883846.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVIAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSAMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            30719..31285
                     /locus_tag="OUB02_000029"
     CDS             30719..31285
                     /locus_tag="OUB02_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HCT8883847.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(31414..31650)
                     /locus_tag="OUB02_000030"
     CDS             complement(31414..31650)
                     /locus_tag="OUB02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883848.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(31960..35388)
                     /gene="uvrA"
                     /locus_tag="OUB02_000031"
     CDS             complement(31960..35388)
                     /gene="uvrA"
                     /locus_tag="OUB02_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCT8883849.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSIGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSKC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            35707..37941
                     /locus_tag="OUB02_000032"
     CDS             35707..37941
                     /locus_tag="OUB02_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8883850.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            38197..40227
                     /locus_tag="OUB02_000033"
     CDS             38197..40227
                     /locus_tag="OUB02_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8883851.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            40224..40676
                     /locus_tag="OUB02_000034"
     CDS             40224..40676
                     /locus_tag="OUB02_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8883852.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            40678..41733
                     /locus_tag="OUB02_000035"
     CDS             40678..41733
                     /locus_tag="OUB02_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCT8883853.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            41800..42108
                     /locus_tag="OUB02_000036"
     CDS             41800..42108
                     /locus_tag="OUB02_000036"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCT8883854.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            42212..43477
                     /locus_tag="OUB02_000037"
     CDS             42212..43477
                     /locus_tag="OUB02_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HCT8883855.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            43565..44281
                     /locus_tag="OUB02_000038"
     CDS             43565..44281
                     /locus_tag="OUB02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCT8883856.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(44476..44769)
                     /locus_tag="OUB02_000039"
     CDS             complement(44476..44769)
                     /locus_tag="OUB02_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HCT8883857.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(44775..44990)
                     /locus_tag="OUB02_000040"
     CDS             complement(44775..44990)
                     /locus_tag="OUB02_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883858.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(45402..45914)
                     /locus_tag="OUB02_000041"
     CDS             complement(45402..45914)
                     /locus_tag="OUB02_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8883859.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            46364..46840
                     /gene="purE"
                     /locus_tag="OUB02_000042"
     CDS             46364..46840
                     /gene="purE"
                     /locus_tag="OUB02_000042"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCT8883860.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            46846..47544
                     /locus_tag="OUB02_000043"
     CDS             46846..47544
                     /locus_tag="OUB02_000043"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCT8883861.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            47556..48923
                     /gene="purF"
                     /locus_tag="OUB02_000044"
     CDS             47556..48923
                     /gene="purF"
                     /locus_tag="OUB02_000044"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCT8883862.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            48917..49993
                     /locus_tag="OUB02_000045"
     CDS             48917..49993
                     /locus_tag="OUB02_000045"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCT8883863.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            49987..50580
                     /locus_tag="OUB02_000046"
     CDS             49987..50580
                     /locus_tag="OUB02_000046"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCT8883864.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKIKLQGRRVFINE"
     gene            50573..52105
                     /gene="purH"
                     /locus_tag="OUB02_000047"
     CDS             50573..52105
                     /gene="purH"
                     /locus_tag="OUB02_000047"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCT8883865.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            52116..53366
                     /gene="purD"
                     /locus_tag="OUB02_000048"
     CDS             52116..53366
                     /gene="purD"
                     /locus_tag="OUB02_000048"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCT8883866.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            53391..57197
                     /locus_tag="OUB02_000049"
     CDS             53391..57197
                     /locus_tag="OUB02_000049"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCT8883867.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            57403..58275
                     /gene="rfbD"
                     /locus_tag="OUB02_000050"
     CDS             57403..58275
                     /gene="rfbD"
                     /locus_tag="OUB02_000050"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCT8883868.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            58384..59253
                     /gene="rfbA"
                     /locus_tag="OUB02_000051"
     CDS             58384..59253
                     /gene="rfbA"
                     /locus_tag="OUB02_000051"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCT8883869.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGINITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            59302..59859
                     /gene="rfbC"
                     /locus_tag="OUB02_000052"
     CDS             59302..59859
                     /gene="rfbC"
                     /locus_tag="OUB02_000052"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCT8883870.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            59897..60880
                     /gene="rfbB"
                     /locus_tag="OUB02_000053"
     CDS             59897..60880
                     /gene="rfbB"
                     /locus_tag="OUB02_000053"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCT8883871.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVNSYYRTYGLLTTISRCSNNYGLYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKVILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            60910..61578
                     /locus_tag="OUB02_000054"
     CDS             60910..61578
                     /locus_tag="OUB02_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCT8883872.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSTGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            61587..62015
                     /locus_tag="OUB02_000055"
     CDS             61587..62015
                     /locus_tag="OUB02_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HCT8883873.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            62039..62353
                     /locus_tag="OUB02_000056"
     CDS             62039..62353
                     /locus_tag="OUB02_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCT8883874.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            62505..62780
                     /gene="flgM"
                     /locus_tag="OUB02_000057"
     CDS             62505..62780
                     /gene="flgM"
                     /locus_tag="OUB02_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HCT8883875.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            62785..63201
                     /locus_tag="OUB02_000058"
     CDS             62785..63201
                     /locus_tag="OUB02_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HCT8883876.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKKTTTYNSYGNIRK"
     gene            63219..64529
                     /gene="flgK"
                     /locus_tag="OUB02_000059"
     CDS             63219..64529
                     /gene="flgK"
                     /locus_tag="OUB02_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCT8883877.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            64548..65480
                     /gene="flgL"
                     /locus_tag="OUB02_000060"
     CDS             64548..65480
                     /gene="flgL"
                     /locus_tag="OUB02_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCT8883878.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            65496..65888
                     /locus_tag="OUB02_000061"
     CDS             65496..65888
                     /locus_tag="OUB02_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HCT8883879.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            65882..66094
                     /gene="csrA"
                     /locus_tag="OUB02_000062"
     CDS             65882..66094
                     /gene="csrA"
                     /locus_tag="OUB02_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCT8883880.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            66116..66511
                     /gene="fliS"
                     /locus_tag="OUB02_000063"
     CDS             66116..66511
                     /gene="fliS"
                     /locus_tag="OUB02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCT8883881.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            66511..66867
                     /gene="fliS"
                     /locus_tag="OUB02_000064"
     CDS             66511..66867
                     /gene="fliS"
                     /locus_tag="OUB02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCT8883882.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            66888..68411
                     /gene="fliD"
                     /locus_tag="OUB02_000065"
     CDS             66888..68411
                     /gene="fliD"
                     /locus_tag="OUB02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCT8883883.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            68414..68749
                     /gene="fliT"
                     /locus_tag="OUB02_000066"
     CDS             68414..68749
                     /gene="fliT"
                     /locus_tag="OUB02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HCT8883884.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            68851..69723
                     /locus_tag="OUB02_000067"
     CDS             68851..69723
                     /locus_tag="OUB02_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HCT8883885.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            69816..71957
                     /locus_tag="OUB02_000068"
     CDS             69816..71957
                     /locus_tag="OUB02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT8883886.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            71981..73858
                     /locus_tag="OUB02_000069"
     CDS             71981..73858
                     /locus_tag="OUB02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT8883887.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFKRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            73851..75965
                     /locus_tag="OUB02_000070"
     CDS             73851..75965
                     /locus_tag="OUB02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCT8883888.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            75974..76969
                     /locus_tag="OUB02_000071"
     CDS             75974..76969
                     /locus_tag="OUB02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HCT8883889.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            77061..78293
                     /locus_tag="OUB02_000072"
     CDS             77061..78293
                     /locus_tag="OUB02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCT8883890.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            78313..79257
                     /locus_tag="OUB02_000073"
     CDS             78313..79257
                     /locus_tag="OUB02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HCT8883891.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            79351..79758
                     /locus_tag="OUB02_000074"
     CDS             79351..79758
                     /locus_tag="OUB02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HCT8883892.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            79845..80936
                     /locus_tag="OUB02_000075"
     CDS             79845..80936
                     /locus_tag="OUB02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HCT8883893.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      81245..81335
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            81745..82062
                     /gene="flgB"
                     /locus_tag="OUB02_000076"
     CDS             81745..82062
                     /gene="flgB"
                     /locus_tag="OUB02_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCT8883894.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMTTLQYNALISFANGKIAMTKSII
                     SGR"
     gene            82070..82474
                     /gene="flgC"
                     /locus_tag="OUB02_000077"
     CDS             82070..82474
                     /gene="flgC"
                     /locus_tag="OUB02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCT8883895.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKKVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            82484..82798
                     /gene="fliE"
                     /locus_tag="OUB02_000078"
     CDS             82484..82798
                     /gene="fliE"
                     /locus_tag="OUB02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCT8883896.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNKEEKKNFEDVIIDTID
                     KVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKV
                     QI"
     gene            82823..84373
                     /gene="fliF"
                     /locus_tag="OUB02_000079"
     CDS             82823..84373
                     /gene="fliF"
                     /locus_tag="OUB02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCT8883897.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTSGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVNENNQPINSGISVDKNKASEQALNKQLEKSILEILEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            84386..85456
                     /gene="fliG"
                     /locus_tag="OUB02_000080"
     CDS             84386..85456
                     /gene="fliG"
                     /locus_tag="OUB02_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCT8883898.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSMSGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            85449..86180
                     /locus_tag="OUB02_000081"
     CDS             85449..86180
                     /locus_tag="OUB02_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCT8883899.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYNEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            86184..87503
                     /gene="fliI"
                     /locus_tag="OUB02_000082"
     CDS             86184..87503
                     /gene="fliI"
                     /locus_tag="OUB02_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HCT8883900.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVYATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRGIFE"
     gene            87535..87981
                     /gene="fliJ"
                     /locus_tag="OUB02_000083"
     CDS             87535..87981
                     /gene="fliJ"
                     /locus_tag="OUB02_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HCT8883901.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSIGKNNDSVQNQKITINYLLALNNSIMDLSEKLDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            88009..89214
                     /locus_tag="OUB02_000084"
     CDS             88009..89214
                     /locus_tag="OUB02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HCT8883902.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNDKFEDNLYKTTDHKKV
                     KDRRETSKQNVSSDRPENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            89232..89840
                     /locus_tag="OUB02_000085"
     CDS             89232..89840
                     /locus_tag="OUB02_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HCT8883903.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            89867..90850
                     /locus_tag="OUB02_000086"
     CDS             89867..90850
                     /locus_tag="OUB02_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HCT8883904.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            90863..91066
                     /locus_tag="OUB02_000087"
     CDS             90863..91066
                     /locus_tag="OUB02_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HCT8883905.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            91066..91884
                     /locus_tag="OUB02_000088"
     CDS             91066..91884
                     /locus_tag="OUB02_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HCT8883906.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            91874..92569
                     /locus_tag="OUB02_000089"
     CDS             91874..92569
                     /locus_tag="OUB02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCT8883907.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            92581..93024
                     /locus_tag="OUB02_000090"
     CDS             92581..93024
                     /locus_tag="OUB02_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HCT8883908.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            93039..93413
                     /locus_tag="OUB02_000091"
     CDS             93039..93413
                     /locus_tag="OUB02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HCT8883909.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEVLKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            93414..94079
                     /gene="fliP"
                     /locus_tag="OUB02_000092"
     CDS             93414..94079
                     /gene="fliP"
                     /locus_tag="OUB02_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCT8883910.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            94092..94361
                     /gene="fliQ"
                     /locus_tag="OUB02_000093"
     CDS             94092..94361
                     /gene="fliQ"
                     /locus_tag="OUB02_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCT8883911.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            94373..96187
                     /locus_tag="OUB02_000094"
     CDS             94373..96187
                     /locus_tag="OUB02_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HCT8883912.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYSVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFNMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            96196..98271
                     /gene="flhA"
                     /locus_tag="OUB02_000095"
     CDS             96196..98271
                     /gene="flhA"
                     /locus_tag="OUB02_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCT8883913.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            98246..99262
                     /gene="flhF"
                     /locus_tag="OUB02_000096"
     CDS             98246..99262
                     /gene="flhF"
                     /locus_tag="OUB02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HCT8883914.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDAM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            99259..100137
                     /locus_tag="OUB02_000097"
     CDS             99259..100137
                     /locus_tag="OUB02_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HCT8883915.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNVISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPELTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            100151..100852
                     /locus_tag="OUB02_000098"
     CDS             100151..100852
                     /locus_tag="OUB02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HCT8883916.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            100868..101290
                     /locus_tag="OUB02_000099"
     CDS             100868..101290
                     /locus_tag="OUB02_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HCT8883917.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            101275..101460
                     /locus_tag="OUB02_000100"
     CDS             101275..101460
                     /locus_tag="OUB02_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HCT8883918.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            101501..102274
                     /gene="flgG"
                     /locus_tag="OUB02_000101"
     CDS             101501..102274
                     /gene="flgG"
                     /locus_tag="OUB02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HCT8883919.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            102295..103056
                     /gene="flgG"
                     /locus_tag="OUB02_000102"
     CDS             102295..103056
                     /gene="flgG"
                     /locus_tag="OUB02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HCT8883920.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            103079..103960
                     /locus_tag="OUB02_000103"
     CDS             103079..103960
                     /locus_tag="OUB02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCT8883921.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            103963..104337
                     /locus_tag="OUB02_000104"
     CDS             103963..104337
                     /locus_tag="OUB02_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCT8883922.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            104369..>105136
                     /locus_tag="OUB02_000105"
     CDS             104369..>105136
                     /locus_tag="OUB02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883923.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISSGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFERYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILG"
BASE COUNT        41887 a        11157 c        19014 g        33078 t
ORIGIN      
        1 ttttaatagt taaattatag tcaattataa taaagctatt taatctactt ttttaatata
       61 aaaatataag ggggaatttg taatggcttg taacacttgt aaaatgtgcg aaagtgcaga
      121 caagaaatta gaaagttttg tagcttcaaa ggatgttgaa acagcatttc atagaacaga
      181 agatcagaaa gttaagtgtg gttttggact tcagggggtt tgttgtagac tctgttcaaa
      241 tggaccatgt agagtaactc ctaaatcacc aagaggtata tgtggagcag atgcagacac
      301 tatagtagca agaaactttt taagagcagt agcatcagga gcagcttgtt atttacatgt
      361 ggtagaaaat acagctaaga atttaaaaaa tgttggaata actaaaggtg ttgttaaagg
      421 agaaaaaaca ttaaatcaat tagcagagat gtttggaata gaaagtgatg aaaagtatga
      481 taaatgtata aaggttgcag acaaagttat caacgatttg tataaatcaa gagatgataa
      541 aatggaatta gtagaaaaaa tagcttatgc acctagagtg aaaaagtgga aagagttagg
      601 tataatgcct ggtggtgcaa agtcagaagt ttttgatgca atagtaaaat catcaacaaa
      661 tttaaatagt gacccagtag acatgctagt taattgtttg aacttaggta tatcaacagg
      721 attatatggt cttacactaa ctaatctttt aaatgatgtt atgttgggag aaccagttat
      781 aagaatggct cctgtaggat ttaatgtaat agacccagac tacataaata taatgataac
      841 aggccatcaa cattcaacat ttgcaaattt ccaagataga ttaaaagatg aagatgttat
      901 aaaattagct caatctgttg gtgcaaaagg ctttaagcta gtaggatgta catgtgttgg
      961 tcaagattta caattaagag gagaacatta tcaagaagta tttgctggac atgcaggtaa
     1021 caattttaca agtgaggctg ttttatcaac tggagcaata gatatagttc tttcagaatt
     1081 taactgtact ataccagggc ttgaaccaat agcagataaa tataaagtaa aaatggtttg
     1141 tttagatgat gttgctaaaa aagcgaatgc agaatacata ggtttagaca gaagtaaatt
     1201 agatgaactt agtaacactt taatagaaaa agcattggag tcttataagg agagaagagg
     1261 aagtattgaa atagatatac caaaagacca tggatttgaa caatctttaa ctggtgtaag
     1321 tgaaaagaac ctgaaagcgt ttttaggaga ttcatggaaa cctcttatta atttaattgc
     1381 agaaggaaaa attaaaggtg ttgcagctgt tgtaggatgt tcaaatatga cagcaggagg
     1441 acatgatgta aatactgttg aacttactaa agaattgatt aaaaaagata taatagtact
     1501 atctgcagga tgttctactg gaggattaga aaatgttggt cttatgtcac ctggagcaga
     1561 agaattagca ggagaaaact taaaagaagt atgtaagact ttaggaatac caccagtatt
     1621 aaactttgga ccatgtctag ctataggaag actagaaata gtagcaacag aattagcagc
     1681 tgaacttgga attgacctac cacaattacc attagtgctt tcagctccac aatggttgga
     1741 agaacaagca ttagctgatg gagcgtttgg tcttgccctt ggcttacctc ttcatttagc
     1801 tttacctcca tttataacag gaggaaaatt ggtgacagaa gtattaacag aaaaattaaa
     1861 agacttaaca ggaggacatg ttatagtgaa tccagaccct aaatcttcag ctaatcaatt
     1921 agaagaaata ataatagata gaagaaaaaa tttagggtta aaggatgtgg aatgcaatgc
     1981 atagaatttt tataaataaa gatttatgta caggatgtaa gagctgtgta ctagcatgta
     2041 tgctaaaaca caataaagat tatgacatgt acactcttga tttagaaaat atagataatg
     2101 atagtagagg gcatatagaa ttagatagta aacataataa tccagttcca attctttgta
     2161 gacactgtga tgaacctgaa tgtgtattag catgtatgag tggtgcaatg cataaagata
     2221 gtgaaagtgg aatcgtttca tatgatgaag agaaatgtgg ttcatgttat atgtgtgtaa
     2281 tgtcttgtcc atatggatta ttaaagccag atgatagaag caagcagaac atacttaaat
     2341 gtgatttatg taaagatgag gagtatccta gatgtgtagc taattgccca agtggagcaa
     2401 ttgaattaca gaaggaggaa aatgatgaat tatgtagtgt taggagctag tgcagctggt
     2461 ataaatgcag taaaaacatt aagagaatta gataaagatt caaatatagt agtaatttct
     2521 aaagatgaaa acgtatactc aagatgtatg ttgcatcacg ttatatctga acacagaact
     2581 ttaaaacaaa taaattttgt tgatgaagat tttatggagc aaaataatgt aaaatggatt
     2641 gcaggtaaga ctgttaaagg tatagacata aataaaaagg tagttcagac agaagacatt
     2701 actgtaaatt atgataaact tttaatagct acaggtgcat cttctgctat tcctcctata
     2761 aaaaatttaa gagatggaaa ttttgtatat tctgtgagaa acatagatga tatttataaa
     2821 ataaaagaaa aagcacaaaa ttctaaaaat gtagttataa taggtgctgg tttagttggt
     2881 atagatgcat tagtgggatt atttaaatat gagcagttaa atatttctgt agcttttatg
     2941 gaaaagtaca ttttagatag acaattggat gaatatacag catcagtgta tgaaaataag
     3001 ttcaaagaaa aaggtgtaaa attttaccca agtgcatcaa tacaagaaat tgtattggat
     3061 aacagtaaga atgttacagg tgttgcattt tcaaatggtg aagttttaga tgcagacatg
     3121 gtaattgtag ctacaggggt taaaccaaat gctgactttt tagatggaac aggaatagaa
     3181 tatgatagag gtataataat agatgatatg tgccaaacaa cacaaaaaga catatatgca
     3241 gcaggagatg ttgtaggtaa aaatgctatt tggcctctgg cagtaaagca aggaattgta
     3301 gctgcctata atatggttgg aaaagataag aagatagaag atgaatttgc ttttaaaaat
     3361 agtatgaatt tcatggatat accaactatt tctataggaa tgaatacacc tgtagatgat
     3421 agctataaag tattaaccag acatggaatt aatgattata aaaagtttgt ttttaaagat
     3481 aatgtaatct atggagcagt tatacaagga gatatttcat atgtaggtgt attaacttat
     3541 ttaataaaaa ataaagttga aatatatgat ttagaaaata ggatatttga tatatgttat
     3601 gcagactttt ttaatataaa agaaaatgga gaattttgtt atagtgtata gtatatacgt
     3661 ggagtaaggt ttataaatac agtaaatcaa taaatagagt tattctaaaa taagaaaaat
     3721 gacaaatctt atttagaaat agctctattt tttgtaatta aatttaaatt atattcaatt
     3781 tgtaaaatat tattttgaac aattatacat atactctcta gttagaggta aatcattttt
     3841 tataccttta gtgaaaagta tttgatgtat atctataata ccagtgttga aagcagcagc
     3901 acaagaacaa aggtataaat cccacattct tataaactta tcatcaaaca tttctttaat
     3961 ctttggaaga ttctcagaga aatttttttg ccagtatagt aaggttttta cataatgtaa
     4021 tcttagactt tctacgtcta tagtatgata tttgtaatca ctacaaatat gtatcatttc
     4081 tcttaggcta ggaataactc ctcctgggaa aatatatttt tttatccaag ggtctccata
     4141 agattcctgc aatccactta tatagtgaag taaaaacaat ccaccatctt ttaaaacagc
     4201 atttacgttt ttaatgaaaa gttcatagtt ttctctacca acatgttcaa gcatcccaac
     4261 gcttacaact ctatcaaaca taaggtctga tttttttaat tctctatagt ccataagtct
     4321 tacctcaaga tagtcttgaa ggttctcttc tttaattctt gtgttaaatt ccttaaattg
     4381 ctcttcgcta agtgtaattc caacgccgtg aattttatat ttttttgcag cttctattag
     4441 taaaaatccc caaccacaac caatatcgag aaggctcata ccttcctcta aatttagttt
     4501 agttaatatg tggtgaactt tattgacttg agcatcataa agagtatcat tatcatttct
     4561 aaagtatgca caagaataac taagtgtctt atctaaccat aagctataaa aatcgttgcc
     4621 aatgtcatag tgggcatgta tttctttctt ttgatgtctc attgaatttg atgagtgcaa
     4681 tagatgtttt acagctgaat tatcaatatt gaatttgtct atttgtttta aaagtagatt
     4741 taaagttaag aataaatctc cctcaacttc aatgtctcta tttatgtagg cttctccaag
     4801 tgctaaagat gtacttttta atagttcaga cttgctaata tcattattta ctataacttt
     4861 gaactgaggg gagccctctc caattagaaa ttcttcctca tctttaagcc taactagaaa
     4921 tggtgtttca ccaaatctct ctaaatagtc ttttaaaaaa ttttttgtaa tattcatatt
     4981 accagctcct tctaatgtaa atccagtaag tttccctgct taaaatatat tatagtcctt
     5041 tttttaaaaa agtaaaattt tttcaataaa ttttttattt aaaggaaatt tattttttat
     5101 aaagaaattt catgttgtag tatatttttt gcttataaat tttggtactg gatataatat
     5161 taaactgtta atgatataat aaatttaatc tataaagtaa ataaaatttt gtaataaata
     5221 gttaaagcta ttttataagg agatatgtag gatggaagta tatgctttgg ttgggtcaag
     5281 tggcacaggg aagagcttta aggctttaga gttcgcatat gaaaatgata tagaatatat
     5341 aatcgatgat gggattttaa tatacaaaaa caaggtttta gctgggatat cggcaaaaca
     5401 agcaaatact actatagaag cagttaaaag ggctattttt tataatttgg agcatagaga
     5461 agaagttaga gaagcaatta aaaaagaaaa tataagaaaa attcttatta ttgggacatc
     5521 aaaaaaaatg gttaatcaaa ttgtagagag attgtcaatt ggaagggtct gtaagtttat
     5581 aaatatacaa gatataagta ctaaatatga gatagaaata gcaaaacaag ctagaaaaga
     5641 aggaaatcat gtaattcctg ttccagcagt tgagataaaa agtatggcaa gtggtcttag
     5701 tataaattct ttgaagagac tttttagaaa aggaaataat aggaatatgg tagttttaga
     5761 aaaaacaatt ataagaccaa cttttagtta tataggaaag ttttatatta gtgctgatgt
     5821 aattaaacag attattgaat atgaagtata caactttgga agcattgata gaataaataa
     5881 aatcaatata gagaattgca ataattttat gaatattttt gtaagtataa atatcaatga
     5941 tttagaaact ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt ctattgagaa
     6001 aatgacttta gttaatgtac aaaaaataga tattaacatt cataaaatta aaaaaataaa
     6061 cttcctccaa tgttaaaaaa taaactgaga aaatgatata ctaatttgca ataaagtgtg
     6121 ctataattaa actgtaaatt taggaaatgg ttttttctaa atggagtata cataattata
     6181 acgtatttat aagggggctt tcaaatgtca ggaaaagatg taaatgtctt cgagatggcg
     6241 caatctcaag taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt ttatgaatta
     6301 ttaaaagaac ctatgagagt tatagaggtt tcaattccag taaaaatgga tgatggttct
     6361 ataaaaactt ttaaaggatt tagatcacaa cataatgatg cagtagggcc aacaaaaggt
     6421 ggaataagat tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc tatatggatg
     6481 actttcaagt gttctgtaac aggtatacca tatggtggag gtaaaggtgg aataatagta
     6541 gatccatcaa ctttatctca aggtgaatta gaaagattaa gtagaggata catagatgga
     6601 atatataaat taataggtga gaaagttgac gttcctgcac cagacgtaaa tactaatggt
     6661 caaataatgt cttggatggt tgatgagtac aataaattaa ctggacaaag ttctataggt
     6721 gttataactg gtaagccagt tgaatttggt ggttctttag gaagaacagc tgctactgga
     6781 tttggtgttg ctgttacagc aagagaagct gctgctaaat taggaataga tatgaaaaaa
     6841 gctaaaatag ctgttcaagg tataggaaac gttggttctt atacagttct taactgtgaa
     6901 aaacttggtg gtactgttgt agctatggct gaatggtgta aatcagaagg ttcttatgct
     6961 atatacaatg aaaatggttt agatggtcaa gctatgttag attatatgaa agaacatggt
     7021 aacttattaa acttcccagg agctaagaga atatctttag aagagttctg ggcttcagat
     7081 gttgatatag taataccagc tgcattagaa aactctataa ctaaagaagt tgctgaatct
     7141 ataaaagcta aattagtttg tgaggctgct aatggaccaa ctactccaga ggctgatgaa
     7201 gtatttgctg aaagaggaat agttcttact ccagatatat taactaatgc tggtggagtt
     7261 acagtttctt actttgagtg ggtacaaaac ttatatggat actactggtc agaagaagaa
     7321 gtagagcaaa aagaagaaat agctatggtt aaagcatttg aatctatttg gaaaatcaaa
     7381 gaagagtaca atgttacaat gagagaagct gcttacatgc attcaattaa gaaagttgct
     7441 gaagctatga aattaagagg atggtactaa gatttactct taataaataa taggatataa
     7501 ttatttaaga tataaagggg ctgtacatag tacagccctt aaattatgtg gcttacaatg
     7561 aagtaagcta tttttgtttt aacttataaa gaacttgata aattatgtaa tttagaatgt
     7621 attttttacc accacatgat tacaaattag aggagttaga ttgaattttt agacatgaac
     7681 ttatgcattt taaaagcaaa gactttcatt taaagtatct agtattattt ttaaaaatag
     7741 tatattggtt taatccattt atatataatg gaaaaatata tggatttgga ttgtgaattt
     7801 tattgtgatg agagagtttt aaaaaattgt actgtacaac aaaagcaaga atatgctcta
     7861 actattgtaa attcaatgaa aagaggtaca aatttttcaa ataaatttgt agctggatta
     7921 aataaacaat ctgatattag aaaaagagta ataaacatgt tcagtgaaaa atataaaaat
     7981 ggtatcttaa tggctttgat tataagcttg ttttcttcta ttacattttt taaattcgat
     8041 tcaactagta ttattgaaga gaatgatttc ttagagaggt caagatatac gtttgaagga
     8101 aggcaagaag acgtgtttgt aacaatagat tttatatatg cagatgcacc tataaaactt
     8161 agaaaagagc atgaagaaaa ctgtagagtc aatggaatag taccaaaaga ttcagattca
     8221 atagaaatta ttcctagatt ttataaagat tgtatggatt taaaaaaata agttagactt
     8281 aatattgatg ttgatattat gaatttttta tatttaagca attgaaaatt ttattataga
     8341 ttcaatttta tacgtgttta agtatgaaat ttatagatag ttgaaataat taaatatgat
     8401 tgatgcatag gaatcattac taagctataa ttaattatag gggagtttaa ataaattatg
     8461 agttacacaa ttataaaatg ataaaaatag ttatctgtta caaaaaatgt atctttttaa
     8521 ttaataaaga taatatgaaa acttaaaagg caaataaact taaacataaa atactcttaa
     8581 ataagatagg agaaaaatgt atgtctaact ttattctaaa tgggattttt agaacagtta
     8641 tagtaagtag tctaagcatt cttatgatat tgattttcaa gaaaaatgta tttaaaagat
     8701 ttagcaaaaa atttaattat tatatatgga tgatagttgt tattaaatta tttttaccat
     8761 ttacatatta tacttttaca ataaatatct tgagaagtaa aaaacacatt aatattaata
     8821 acataaattt agagggattt aataatgttt caactataag taataatatt atattgtaca
     8881 cttggattat aactgtagta gtttatttaa tttacactat atttaaatat ataaaactta
     8941 aaaatttaat aaatgactta tcttatgatg ttgatgatgg agagattgta aatctttata
     9001 aaaatatatt agaagaattt aatataacaa aagatataaa actcaaatac tcttatgaag
     9061 ttgaaacacc agcatttttt aattcatgtg tgcttttgcc accacatgag tataaattga
     9121 aagagttaga ctggattttt agacatgagc ttatgcattt taagagtagg gacctttatt
     9181 taaaatatgt aatcctgttt ttaaaaacag tatattggtt taatccattt atatatataa
     9241 tggataaaca tatagattta gattgtgaac tttattgtga tgaaagagtt ttaaaaaatc
     9301 gaaattcaga tgaaaaaaag gattatgctt taacaattct aaatgcaatg agaaaaggtt
     9361 caaatacttc aaataaattt atagctggac tccataagca atctgatatt aaaaagagag
     9421 ttatgaatat gtttaatgaa aagtacaaga atggtatatt aatggcatta actttatgtt
     9481 tattatcttc tattactttt ttgaaggtta attcaacgag tattgttaat cttaatatat
     9541 tgcctaaata tgatatggag aagctacatg tttttagagg aagtgttaca cttttagaat
     9601 ttacatatgc agatgctcct gaaaatgtta aaaaagaaca taaaagaatg tatgaagcaa
     9661 tgggattgat tcctaaagac tctgataaaa taagctttgg tgtaaaatac tatgacaagt
     9721 tgaacaattt attaagatag atatttatat aatagcaaat agctgaaact taaaagaatt
     9781 taaaagtaag tattcacaaa agtcctaaag aaagtattaa taattatatt caattgtaaa
     9841 aaatttcaca aaacaaccaa cttttatagt tatttcatca aaaaatatac gccatataaa
     9901 agcgcataaa aaaacacata aaacaatatt tatgcgcact ttaacctcga atggtacaaa
     9961 attctttact tttacagacc catattatat aattaagtaa ataatgtata tttaggtgac
    10021 atattggata agctaattta tttattttat taatataata atactataaa agttatataa
    10081 agtggtttac aaaaaagtta cagatagagc ttttattaat gtattgtgta ttagtatttt
    10141 atccttaggt acacttcacc tacaaaatag aatcataaga taattatggg aaaattttag
    10201 gaggtttatc gtgaagaaaa agatattaat accagtattt gcatcagtta tggcattatc
    10261 agtaagttca attgtaaatg cagatgaagt aaatgattca tctcaaaata aggatgataa
    10321 aacaaatact gaattaaatt taggtgaata taaagaagta aaatataaag ttgcaaaaat
    10381 aaaagatgga gtagctataa agataagaga agaaggtcaa gttcaaaaca tagcttattc
    10441 tggagatgaa tttacggttt taggaactca aggagaatgg gttaaggtta aagttgaaga
    10501 tggcgaaggt tggttagcta ctagatatgt agatatatct gaaggtgtag gatatactaa
    10561 cgctgataaa gtaaacttaa gaaaagataa gagtgaaagt tcagaagtaa ttgaagagct
    10621 ggaaaaagga agttctttat tggttttgga agataatgga gattggttaa aagttaaaga
    10681 tggggaaaca gaaggatatg taaaatcatc ttatatatct gataaagctc ctgtaataga
    10741 agagccacaa gtaaacccaa gtattgacca aaatgtagat tctacaaata ataatcaaca
    10801 aaacaatgca aatactaatc aacaaaataa taattcaaat gtgccaacag ctaactctaa
    10861 tgcagttcaa gctgtactta acttggcata ttctaagcaa ggttgtccat atgtttgggg
    10921 agcagaagga ccaaacactt ttgactgttc tggatttaca caatatgtgt atagaaatgc
    10981 agtaggaaag aatataccaa gaacatctaa agcacaatct aaatatggac aaactgtaag
    11041 taaagcaaat ttacaaccag gagacttagt attcttcaca actaatggtt ctggttctgt
    11101 tagtcatgtt ggaatatatg taggtggggg taatatgata cattcaccat caacaggaaa
    11161 aacagttagt gtaactagta taaattcatc atactataca gcaagatttg ttacagcaaa
    11221 aagaatatta taatcggttt gtaatatgac aaattgataa atacataaat atatataaac
    11281 gctctatatt atatttaatt taatcatgga aacaaaaaat gattatattt taaatgtact
    11341 atagagcgtt tttgtatcac tttaaatcta atcaagattc aagttaatat gactgttaaa
    11401 taggagcctt ttcatatgat gattatttgc gcaatccctt gacaggatag ggcaaaggtg
    11461 gtaagctact aacagtacaa aaatactttt aattttagtt cagagaggct aaaaaaggag
    11521 gacgaaagat gttaaaatca agaaatttaa tccaaccaga agacttttca atagaagaaa
    11581 tagatgaaat attagaatta gctcaaaaaa taattgacaa tccgtcaaaa tattctcgta
    11641 tatgtgaagg aaaactatta gctacattat tttatgaacc atcaacaaga acaagattaa
    11701 gctttgaatc tgctatgaac agattaggag gacgtgtagt aggcttttca gagccaaatt
    11761 catcctctgc atcaaaaggt gaaactctag gagatacaat gaggatagta tctggttatg
    11821 ttgatataat agctatgaga catcctcaat caggtgcagc ttcagaagca gctagatata
    11881 cggaagtgcc atttataaat gcaggagacg gcaagaacca gcatccaact caaacactta
    11941 cagacttact tactataaaa tcattaaaag gaactttaga aagccataca ataggattat
    12001 gtggtgattt aaaatatgga agaactgtgc attctttggt taaggctatg gcaagatata
    12061 aaaatacaaa gtttgtattt atagcgcctg aagagttaaa aatgccagat tatataaaag
    12121 aagctataaa gggacatgca tattatgaaa ctaataattt ggatgatgtt ataggaagct
    12181 tggatgtttt atatatgact agagttcaac aagaaagatt tgaagataaa tcagagtatg
    12241 aaagacttaa aaactattat attttaaata aagctaaatt agaaaaggca tctaaagata
    12301 tgttagtaat gcatccatta ccaagagtaa atgaaataga catagatgta gatagtgatg
    12361 atagagctgt atattttaaa caagctaaat atggaatgta tgttagaatg gctttaataa
    12421 taaaactttt aggtataaat gaagactaat agttatcata taatagtaaa acaaaggaga
    12481 ttttgggatg tataaaattc tggaaaatat atatataggc gaagatatgt atagaatgaa
    12541 agtaaaaggt aattttgaag gtaaaatggg acaattctat atgttaagag catgggatac
    12601 ttatccagta ctttcaagac ctataagtat acatgatata gatgaagaag ggataacttt
    12661 tctttataaa gtagtaggag aaggaactca aattttgagt aacctaaagg taaatgataa
    12721 tataaaatta gaaggccctt atggtaatgg atatgctaag gttgatggaa aggtagctct
    12781 agtaggagga ggaatcggtg tagctccact atatttggtt gctaaaaata taaaaaattg
    12841 tgatgcatat cttgggttta gagaagatgt tatattagaa gatgaatata aacaagtatg
    12901 taataaagtc tatacaacag taggaaatac ttttgtaaca gatataattg atgttgagaa
    12961 atatgattat atattaacat gtggtccaac tccaatgatg gaaaaattag ttaagatggt
    13021 agaaggtact aaaacgagaa ttatggtatc tcttgaaaac catatggctt gtggagtagg
    13081 tgcctgttta gtatgtacgt gtaaaactaa tggaggaaat aaaaagactt gtaaagatgg
    13141 tccagtgttc tggggagagg acgtgatttt taatgggtaa tttaagtgtg aaatttggaa
    13201 gtgtagaatt taaaaatcca gtaataatgg cgtctggaac ttttggattt ggaaaagaat
    13261 ataatgagat ttatgatata caaaaacttg gtggtataag tagcaagggt cttactttaa
    13321 ataaaaaacc tggtaacaat ggtatgagag ttcatgagac accatccggt atgatgaata
    13381 gtgttgggct tgaaaaccct ggagttcaag gatttataga ctatgaatta ccttttttta
    13441 gtaaactaga cttagtcaga atagctaatg ttggaggtgg gacattagaa gactacttat
    13501 taggggttca aatgttaaat gacaagccta tagacattat tgaattaaat atatcttgcc
    13561 caaatgtcaa agctggtgga atggcttttg gtataaaaaa tgaagtggca agagaagttg
    13621 ttcgtgaggt aagaaacata acgaaactgc ctttagtaat aaaattatca cccaatgcag
    13681 aagatattgt aggaatggca aaagtttgtg aagaagaagg tgctgatgga gtttcattag
    13741 ttaatacttt taaagcaatg gcaattgata taaaaaatag aagaccagta tttgaaaatg
    13801 tatatgctgg gttatctgga ccagctataa aacctatagc tttgagaatg gtacatgaag
    13861 tgtgtaaaaa tgtaaatata cctgtaatgg gaatgggcgg aataactaaa gccacagatg
    13921 ctatagagtt tataatggca ggagctactt gtatccaagt tggaactgca aattttatga
    13981 atcctagaat aggtattgaa ataatagatg gaattaatga atttatggat agagaaggaa
    14041 taaaaagttt agacgaaata agaggaatta tataaggtag aataaatagt attttaaaga
    14101 taaaaaatat agattaaata aacaataatt ttataacatt aacatggagg aaaaataata
    14161 tgagtaaagt agatgtagtg gatatattaa agaaaagtga tgcattgttg gagggacatt
    14221 ttttattatc ttcaggaaaa cacagtaata gatacgtaca atgtgcaaaa gtattaagat
    14281 ttccagaata tgctgctgag gtattaagta cagttgttga acaaataaaa gacttagata
    14341 tagacttagt agtaggacca gctatgggtg gagtaatagt ttcttatgag ttaggaagac
    14401 aattaggaaa agaagctgta tttactgaga gaaaagacaa tacaatggag ttaagaagag
    14461 gatttgaagt taaaaaagga gcaaagataa taattgctga agacgttgta actactggta
    14521 aatcaactat ggagacaaaa agagtattag aagccttagg tggagaagtt gtaggtgttg
    14581 catgtatagc agatagaact aatcatgata taggtatgcc tatatatagt gctataaaac
    14641 ttgatattca agtttatgaa tctgatgagt gtcctttatg taaggaagga aaattaccag
    14701 ttgttaagcc tggaagtagg gagttcaagg aattagggat gtaataaaaa cttaattatt
    14761 tatagtgtta cttaaaaaat gtaaattttt tagagtaaaa atttgtaata taacatataa
    14821 cttaaaaata aaaagcagtt agccttataa atgaaggtta gctgtttttt ttgttaaaaa
    14881 tatatttaca aaataagaga tagtagcagt acaatataag tataaggtaa aagtattata
    14941 tattagggag gggtaagtat gaaaaatcaa ttaaatgaaa atcataaaaa ggttgtattt
    15001 tttggagtag gtgctgtagg agctactttt gcagaacaat tttttaactc taaatatgat
    15061 tttaaaattc tttgtgataa tgaaagaaag aaaagatatt tagaagaagg atttataata
    15121 aatggaaaaa ggtatgattt tgattatgta actaaagatg agtataaaca agaggctgat
    15181 tttataatta taggtctaaa atataataat ttaaaagaaa atataaaaga attagatgga
    15241 ttagttggaa aaaatacagt tataatgtct ctgctaaatg gagttgatag cgaagagata
    15301 ataggagaaa gatttggaat tgaaaaaatg gtatattcat atgttaccaa tatagatgca
    15361 aagaaaatca ataataatat tatacacact actaatggga taattgtatt tggtaacaaa
    15421 gataatagtg aagatagaaa aactaatata ataaccgaag tctttgatga tgtaaatata
    15481 gaatatactt tatcaaaaga tattcaacga gatatgtggt ggaagtacat ggttaatatt
    15541 ggtgtaaatc aaacttcagc tatacttggt gcaccttatg gagtttttca gagttctgag
    15601 catttaagag aattagcaaa atctgcaatg agggaagttg ttgctatagc acaagcaaaa
    15661 gacatatctc ttacagaaga tgatgtggaa cattcattac atagaatact agaacattca
    15721 aaagaaggaa gaacatcaat gcttcaagat gtggaagctc atagacttac agaagtagat
    15781 atgttttcta agaatatctg caaacttgga aaaaaatata acatacctac tcctataaat
    15841 cagactttct tttatatgat aaaagtaatt gaaagtagat tttaaagggt ctttgtaaga
    15901 aatattgata attattgatt ttaaatttta ttaatgttat atactattta agttactaaa
    15961 tatttttaac tcttcaatga aaatagttgc tgcttttgat aaagttatat cttttggaaa
    16021 tatatatgag aaatgtctgt tgtagctgct tccaagctct atgacagaca gctcattgtt
    16081 tagaacaggt aagcttgtca ctagattaga gactattgta attcctaaat tatttctaac
    16141 agattctttg acagcataat tacttccaaa aaccatcata ctttttggaa taatttcttt
    16201 aacacttaaa aacatatcta aataatctct ggttccagaa ccatcctccc tgactatcca
    16261 tttttggttt tgaagtttat caaaactaaa atcttttaat aaatgactct tgtatggaag
    16321 tgctaaaacc atcttatctt caaaaaaata ttcttgaata aatgaagggg aagagcaagt
    16381 gccttcaata agaccaatgt ctaaaatata atccttaaca tgagaagata cgattgatgt
    16441 attttttata aaaacttcta catctatgtc aggatacttt ttagaaaata atgctagaaa
    16501 atttggtaaa atatactctc caatagttaa acttgcacca atctttaagt gaccagttat
    16561 agcatctgaa acttggctta catcatgata agtaatattt aaaagattta atatttcttt
    16621 agctcttttg tacaagattt gtccactttc tgttataaaa atggtttttt gtttaataga
    16681 cctgtttata agcacaactt taaaataatt ttctagattt tttatgtgtt tacttacact
    16741 tggttgtgat agatttaaat attccccagc tttagtaaag tttttgtatt caacaacagc
    16801 tacaaatgtt tttaattctt caaacaattt tttgtctcct ttacctaatg aaattttaca
    16861 ttttttatat actttctaca gataattata ctatatataa taaccattaa aaacattaat
    16921 cattacgata ttatatattt attttactta tggttattac aattatatac tattgctatg
    16981 aagaaaacta tattaattat aaggtaggta tattatgtta aagaaaaaaa gcagtttgac
    17041 attaaaaact ataagggaaa tattaccagg actgtttgta tcagtattag taggttatat
    17101 tagtatattt atttctattt taattcctaa ggtaggggca gcatcaattt ctatattttt
    17161 aggtatgttt gtaggaaacc tatttttaaa tcaaaaggta tttcaaaaag gttataaatt
    17221 ttcagagaca gatttgttat catattcaat agtactatta ggagcaacac tcagtgtatc
    17281 tactttaata gacttaaaag tttcaggaat tttatttata atattgcaaa tgacaataac
    17341 aataatagct gcactttata taggtaaaaa actaggattt gaagaaaact ttagatttct
    17401 aatggcaagt ggaaatgcag tatgtggttc ttctgcaata ggagcaacag ttccagtggt
    17461 taatgcgaca gataaagaaa agggaattgc agtaactatt gtaaatgtta ctggtatatt
    17521 tcttatgttt ttacttccaa taatatctca atttttatat agccatgaac ttgtaaaaac
    17581 ttcagcaatg ataggtggaa ctttgcaatc aattggtcag gttgtagcaa gtggagcaat
    17641 tgttggagaa aatgtaaaag atttagctac tatttttaag atagtaagag taatatttct
    17701 agttgttgtt gtattagtat ttggacatat gaaaaatcaa tctaataggg aaattcttga
    17761 agaagaaaag gatgagataa aaaagaaaaa agtgactata ccatggtatg taattggatt
    17821 ctttataact tgtgcacttt tttcaatgaa cattatacca aaagaagtat ctgtgttgtg
    17881 caaagaaata agtaacaaac tagaaataat agctttagca gcaattggat taaaggttaa
    17941 tgtaaaagat ttagtgaaac aagggaaaga agtttcttta tatggtttat ttgtaggtac
    18001 agtacaagtt gtatcagctg tagttttaat aaaaatattt atatagtata aaaatagtat
    18061 ttgtaatact aatttgatta attaaaataa tatgatacta ataatttata atataaataa
    18121 aaaatgaatt tcgataagtt taagtgggta taaacttata gaaattcatt ttttatttaa
    18181 ggtaaaaatt taaagtaaaa tattatttta cataataata accttataaa ttttgaaatt
    18241 acttatcttt gtgttaaaat aagtaaaatg cacatattag agctaagttt ttaagtaaat
    18301 aaacaaaatg aaaatataaa tattaaggag atttggtata tgaatgttta tgaaaaaggt
    18361 aatggagtta tactagaaag agtaacagat tttgacccag tacatatatt tgagtgtggt
    18421 caatgtttta gatggcacaa acaagaagat ggctcataca ctggagttgc aaaggggaga
    18481 atattaaatg tcaaaaaaga gaatgataaa atatatttaa ataatactaa tttaaaggag
    18541 tttaacagca tttggtataa ttactttgat ttaggaactg attatacaga aataaagaat
    18601 aagctaaaaa atatggatga atatttaaat aaggctactg aatttggatg gggaataaga
    18661 atattaagac aagatggatg ggaaatgctt atatcattta taatttcctc taataatagg
    18721 attcctatga tacaaagagc gatagaaaat ttatcaagaa agtttggtaa gtatataggt
    18781 gaatatgaag gtaatgaata ctatgcattc ccaacaccag aagaactaaa taaggcctca
    18841 caagaggaaa taagagcatg tcaaacaggt tttagagata agtacataaa aagtactaca
    18901 caggcagtta tagagaataa tgatgaggta tctgaatata caaatttaag tacagaggat
    18961 tgtagaaaag agcttttaaa atttaatgga gtaggaccaa aagtatgtga ttgtattgca
    19021 ttatttggta tgcaaaaata tgattctttt ccagttgatg tttgggttaa gagagttatg
    19081 caagaatttt atatagatga agacatgagt ttacctaaaa tgagaactta tggaatcgat
    19141 aaatttaaag aaatgtctgg atttgctcaa cagtacttat tttactatgc aagagaactc
    19201 ggtataggaa aataaattaa gggggagaaa ttatgggagt aataggaaca atatttctgt
    19261 tctatttaat tatatcatat ctagctggag ccataatttc tgtgattata ttacttgaaa
    19321 atagagaccc tgcaaaaaca atgtcttggc tattgatgtt tattatattt ccaggtgtag
    19381 gtttaatgat atatgctatt tcaggtagaa atattagaaa aagaaaatta tttaagactc
    19441 aaaaactagc taataatata aaagaaaaga aactatttga tacattagaa aagataacag
    19501 aaatagttga attagaaaaa gaatctataa aacaaaataa gcttttaaga gatgaagagg
    19561 atggtagtta tagaaaaagg gtaataaata tgttgttaaa gactggtatg tttcccttta
    19621 ctaaaaataa taaagtggat gtatttgtag atggaaatga aaagtttaaa agacttatag
    19681 aagatataag agaagctaaa gaccatattc atttagagta ctttatcatc aaagatagtg
    19741 aaataggtag ggtgttaaaa gaagaactta taaaaaaggc taaagaaggc attaagataa
    19801 gaattttata tgatgatgta ggatgctgga gattttggtt taatagaaaa tttttccgag
    19861 aaatgaggga agtaggtata gaaattgcag catttttacc tacaaaattt cctataatag
    19921 gagggaaatt aaactataga aatcacagaa aaattgttgt tattgatgga ataattggat
    19981 atacaggtgg aataaatata ggagatgaat atttaggcaa aaatgataag tttggatatt
    20041 ggagagatac tcatattagg attaagggta tttctgtata catgcttcaa atgacatttc
    20101 tgattgattg gtattataca acaaaagaag tattagtgac taaaaattat ttcccaagtg
    20161 tagggaatgt tggtgaaagt atgatacaag ttgttgcgag tggtccagac agtgactggg
    20221 aagatattca ttatgcttat ttttctgcaa tatgtcaggc tagaaaaaat gtttatatag
    20281 aaactccata ttttattcca gatgaaagtc tactaaaagc tataaagagt gctgctctta
    20341 gtggagttga tgtgagaata atatttccta aaattgcaga ccacaagata gtaaatatag
    20401 catcttattc atattttgaa gaaatattaa gagcaggtgg taaagtttat ttatataata
    20461 aaggatttat acattctaaa gtcgtaataa tagatgataa aattgcttct gcaggtacag
    20521 ctaatatgga tttaagaagt tttatgctta actttgaggt aaatgcattt atctatgatg
    20581 aagaagttat tagggtaatg acagatgatt tctttgagga tttaagtcac tgtgaagaac
    20641 ttaatttgga ggtatttaag aatagaaata tcatacaaaa aatcaaagaa tctgtagcaa
    20701 gattattttc accaatatta taaaaaatta ttaaaaaagg tattgaaaat tatgtgtgat
    20761 tggtatatta taataattgt tagcactcta ttagatagag tgataataaa taaaaagtat
    20821 atatataaat ctacatatat aagtagtatt aatttaaaaa taactaaaaa cataggcgaa
    20881 ctagtctata cttaatattt taggaggcgt taataatgaa aataagacca ttagctgaca
    20941 gagtagtaat taaaaaagta gaagcagaag aaaaaactgc aagtggaata gttttaccag
    21001 gagcagctaa agagcaacct caaatagctg aagttgtaga agttggacca ggtggaatag
    21061 ttgaagggaa agaaataaaa atggaattga cagtgggaga taaggttata ttccaaaaat
    21121 attctggaac agaagttaag atagaaggac aagaatacac aatactaaga cagagtgatg
    21181 tattagctgt tattgaataa atatagaata aatttattag gaggggttta aaatggctaa
    21241 agaaattaaa ttttcagagg aaacaagaag agctttagaa gctggtgtaa ataaattagc
    21301 agatacagta aaagtaacat taggacctaa aggaagaaat gttatattag ataaaaaatt
    21361 tggttctcca cttataacta atgatggagt aactatagca aaagagatag aactagaaga
    21421 tagatttgaa aatatgggtg ctcaattagt taaagaagta gcaactaaaa ctaatgatgt
    21481 agctggagat ggtactacaa ctgctacagt tttagcacaa gctataataa gagaaggttt
    21541 aaagaatgta acagcagggg ctaacccaat acttttaaga aaaggaatac aaaaagcagt
    21601 aacagtagca gtagaagaat taaaaaatca atcaagaata gtagaaacac aagaggctat
    21661 atctcaagtt gcttctatat ctgctggtga tgaagaagtt ggaaaattaa tagcagaagc
    21721 tatggaaata gtaggtaaag atggagttat aactgttgaa gagtctcaaa ctatgaatac
    21781 tgaattagat gctgttgaag gtatgcagtt tgatagagga tttgtttctg catatatggt
    21841 tacagatgta gataaaatgg aagcagtttt aaatgaccca tatatattaa ttactgataa
    21901 aaaaatatct aacatacaag aattattacc agttcttgaa caaatagttc aacaaggtaa
    21961 aaagttattg ataatagctg aagatgtaga aggtgaagca ttatctacat tagtagtaaa
    22021 taaattaaga ggaacatttg atgtagtagc agttaaagct ccaggctttg gagatagaag
    22081 aaaagaaatg cttcaagata tagcaatact tacaggtgct caagtaatat cagaagaatt
    22141 aggttatgat ttaaaagaag ctgatttatc tatgctaggt agagcttcat ctgttaaggt
    22201 aactaaagaa agtactacaa tagtagatgg ttctggagat aaaaaagcta tagaagatag
    22261 agtaactcaa attaaacatc aagtagagca aactacttca gattttgata gagagaaatt
    22321 aatggaaaga ttagctaaac ttgctggagg agtagctgtt gtaaaagttg gagctgctac
    22381 agaagtagag ttaaaagaaa gaaagttaag aatagaagat gctcttaatg caactagagc
    22441 agctgtagaa gaaggaatag tagctggtgg aggaactgct tttgttagtg ttataccagc
    22501 aataggaaca ctaattgaga gtttagaagg agaagttaaa ctaggtgctc aaatagttaa
    22561 aaaagcgtta gaagagccat taagacaaat agctataaat gctggtcttg aaggtgctgt
    22621 aattgtgcaa aatgttgtta attctgaagc agaaactgga tttgacgcat tgaatgaaaa
    22681 atatgtaaat atgatagaag ctggtatagt tgacccaact aaagttagta gaagtgcttt
    22741 acaaaatgct gcatcaatag caagtacttt cttaactact gaagctgctg ttgctgacct
    22801 tcctgaaaaa gaagatgcag gaatgccagg aatgggtggc ggaatgcctg gtatgatgta
    22861 atttaaaaaa tttaaaatat aaattttaat ataaagaact atttcgactt agattttaaa
    22921 ctctaagttg aaatagttct tttgtatata tctaattttt gtatatctat aaattcaata
    22981 cacatattta gataatcagt atctagtttg taataatatt ttagagtagt attgttattt
    23041 tttaggttat gattttagat atatattaat taaggtataa ttgttgatag agaataaatc
    23101 aaatttaaaa ggatgcgata atatggactt aagaaaattg cttgaagaag ttaaaaatga
    23161 aaatattgat atagattttg cgttagaaaa gttaaaagat ttaccttatg aagaccttgg
    23221 atatgcaaat atagaccatc atagagaatt aagaaatggg tatccggaag ttatttattg
    23281 tgaaggtaag agtgatgagc atattttagg gattattgat aaaatgaatc aaaaaagttc
    23341 taacatattg gggacaaggt gcagaaaaga aacttttctg aaaattaaag aaatctataa
    23401 tcatgctgag tatgaagagg tgtctaaaat tcttaagatt caaaatcatg atatagagaa
    23461 tataggaaaa ggtaaaattg tgattgctac agggggaact tctgatatac cagtagcaga
    23521 cgaagcttat catacagcta agtttttagg taatgatgtt gatagaatat atgatgttgg
    23581 agtggcaggt atacataggc ttttaaataa aagacataaa atagatagtg ctagagtaat
    23641 agtagccgtt gcaggtatgg aaggtgctct tgcaagtgta attggtggac tggttgatgt
    23701 tccagtaata gctgttccaa cttctgttgg atatggagca aattttggag gattagcagc
    23761 attactagct atgttaaata gctgtgcatc tggaatatct gttgtaaata ttgataatgg
    23821 atttggagca ggatatttag catcaatgat aaataaactg taaatacatt gatataaatt
    23881 aaacaaaact atataataaa atttaatttc tattatattt ttactattaa aattgaggag
    23941 acatatatga gtgaaaaaat actttatttt gatataataa gcggtatatc aggggatatg
    24001 acccttgcaa gtttattaaa tttaggtgtg cctaaggaaa tttttttgga ggaaattcat
    24061 aaactaagaa tgagcgatga atttaatata aacatcagct cgaaaactga aaatggaata
    24121 gtaggtacaa aagtagaagt aattacaaaa gaaaaacata cgcatagaaa tttagtggat
    24181 atatttgagg ttatagataa aagcactcta aacgaaaatg taaaatttaa agcaaaaaaa
    24241 atatttatgg taattggaga agcagaagca aaagttcatg gaactacaat agacaagata
    24301 cattttcatg aggtaggggc tatagattca attgtagata tagtaggagc ttctatatta
    24361 gttgacttac tttgtgtaga taaagtttgt gctagtacag tgtctgttgg ttcaggattt
    24421 gtaaaatgtg aacatggaat tatacctata cctgcacctg caactataga aattttaaaa
    24481 ggtgtgccaa ttaaattaaa taatgtaaat ggagaatgta caactccaac aggagctgca
    24541 atcataaaag tcttgtgtga tgagtttgta gatgagcttg aattttctcc aaagcaaatt
    24601 ggatatggta taggtcataa gaagtttgaa gtaccaaata tactcagaac tgtattaggt
    24661 gaaaaaaaaa agaagaatta atttatgaaa ttagtgcaaa cattgatgat atgtcttcag
    24721 aaatctattc ttatttgtat gaaaaaatat tagatgagaa agcattagat atttatacag
    24781 agagcattta tatgaagaag aatagaccag caacaaaagt atcaatttta tgtaatgaat
    24841 cagatttaga aaaatttatt aaaatattac ttttagaaac tagtactttt ggagtgagat
    24901 ataatgcata taatagagaa attctagaga gaaaatttgt aaaattagat acaaaatatg
    24961 gagtggttac agtaaaacta gggtattata aaggaaaact agtcaaggct actccagaat
    25021 ataatgaatg taaaactata gcaaaaaatg aagatattcc tataaaaaaa gtgtttgctg
    25081 atataaattt tagaataaaa gaagaattta gtgaaaactt attgacataa gtaataaatt
    25141 ttgataaatt aatattcagc cttattaaat aaaagtaaac agaaaaaaat atataaaata
    25201 taaactcact cgtatatgct cagaaatatg gtctgagagt ctctaccaag ataccgtaaa
    25261 tatcttgact atgagtgaaa ttattatacc aaaagaccta gttaagtaaa tgtgtaggct
    25321 gagttaaact ccctatgtgt tggctataag ataaaggtat tttggaagcc tgtatagtaa
    25381 tttcactttt gctagaaatt atggaagtta ctatacaggt tttttgtgta aaaagattta
    25441 atataattta gactgtattg tgttaaaata aatatataaa aactatatta tacaaggaga
    25501 gatgtaaatt tatgaaacat gaattagtac ttgtaataga ttttggagga caatataatc
    25561 agttgatagc tagaagagtt agagaaaaca acgtatattg tgaaatactt ccatgtactg
    25621 caagcataga aagaataaaa gaaaaaaatc ctaaagggat aatatttaca ggcggtccaa
    25681 atagtgcgta tttagaagat tctccaacaa tatcaaaaga aatatttgaa ctaggagtac
    25741 caatacttgg aatatgctat ggtattcaaa taatgtctca tgtattgggt ggagttgtaa
    25801 gaaaaggtaa taaacaagaa aaagaatatg gaaaaacagc tataacttat ggaaaatcat
    25861 cattatttga aggaataact acaaatagtg tatggatgag tcatacagat ttaatagaaa
    25921 aagttccaga aggatttact atagttgcaa atactaatga ttgtccagta gcagctatgg
    25981 aaaatgtaga acgaaaccta tatggagttc aattccatcc agaggtagaa cactgtttgg
    26041 aaggggacaa gatacttaca aacttcttat ataatatttg taaagtaaaa ggtgactgga
    26101 ctacagattc attcatagaa gataaaataa aagaattaaa agaaaaaata ggcgataaaa
    26161 aagctttatg tgctttaagt ggaggagttg attcttctgt tgctgcagtt cttattcata
    26221 aagcaattgg ggacaactta acttgtatat ttgttgacca tggtttactt agaaaaaatg
    26281 aaggaaatga tgtagaaaga atatttagag agaaatttga tataaattta ataagagtta
    26341 atgcagaaga tagattctta tctaaattaa aaggagtttc tgaaccagaa gctaagagaa
    26401 aaataatagg tgaagaattt ataagagtat ttgaagaaga atcaaataaa ttaggaaaaa
    26461 tggatttctt agtacaagga acaatctatc cagatgttat agaaagtgga catggaaatg
    26521 ctgctactat aaagtctcat cataatgttg gtggaatccc agaagatgta gacttccaag
    26581 aaattgtaga accattaaga gagttattta aggatgaagt tagaaaaata ggattagagc
    26641 ttggtataga agaaggatta atatttagac atcctttccc aggaccaggt ctaggaataa
    26701 gagttatagg tgatgtaact aaagaaaaat gtgatatatt aagagaagct gatgctgttt
    26761 atatggacga gcttagaaaa gcaggtttat acagagaaat atggcaagct tttgcaacat
    26821 taccagatgt taagactgtt ggagttatgg gtgatgaaag aacatatgct tatttagttg
    26881 gtttaagagc agttacttct tctgatggaa tgacttctga ctggtataag atgccttatg
    26941 atgtattaga aagaatttct aatagaataa taaatgaagt tgatggagta aatagagtag
    27001 tttatgatat aacttctaag cctcctggaa ctattgaatg ggaataaaat atagaatgcc
    27061 aaaaatccac ataaatacag gatttgaggt ctatcttact tctatgttat actggtttga
    27121 tactatcaat atttgccagt atcttatgga ttaataagta aaaaacatcc tgctgaatga
    27181 taaattcagc ggggtgtttt tattttgttt tagaactcct ttgctctgaa actcgaagta
    27241 tttaacactg atgattttgt tatcttttag ttccttttgt gtcatgctct gaaagacaaa
    27301 ttgatacaat aaaaaagtaa tttcttaatc attgatggag atatgtattt atgttactct
    27361 tgatggtaat ttgagtaata ttgttcatag ctttttatat tcacttaatt agaaaaccat
    27421 ttggtataat cataaataaa cttgatgata taatagaaaa gatttatact tattagaagt
    27481 atataaaagt attttatgtc tatgaaatgt atgtctataa aacatataga tgacttttgt
    27541 atattaatgt atgaatattg atattataga agaattgagt tgtcaatgat actaaatcaa
    27601 atatttatag aagctggagg gattatgaga aataaaataa ttataggaat tactttttca
    27661 cttgtattac taatgtctgg atgtgcttca aaacaaaccg cagaaataaa cgtattagca
    27721 actactgatt tacatggagt tataccttat gagttaactt cttatgtaaa agaagaaaga
    27781 aaaaaagata aaaatctaac tttagtagat gcaggagatt tttttgatag tggagaagtg
    27841 tttggttcta gcatggatag ttattttagt gctagatata aagatggtga taataacact
    27901 gaaagttata ttgagacgcc tatttcaaaa gatatgaaag aggtaggtta tgatgttgca
    27961 gttcttggga atcatgaatt tatatccaac aataaatttt atctagataa tatgatatct
    28021 gattttgaga agcaaaaaat aaatatttta tcggcaaata cgtataaaaa aaatggagac
    28081 agttatacaa aaccatacat aataaaaaat attaatacac ctgaaggaaa cgtaaaacta
    28141 ggaattttag ggcttactat aaaagaagtt ggtgagagga aacagtggaa agatggaaaa
    28201 cttgttgact ctaagtcttt agaactaaaa gaccaagagg gctacaatgg tgaattgtat
    28261 atgaatgatt tagtagaaga tgctaaaaag tgggttagta taatggaaaa agagaagcct
    28321 gacattatag ttgctgtggc acatacaggt gagaaaccta agaaacctag aaacccagga
    28381 aatagaatac aagacttagc tcaacaagtt gatggaattg atgctatagt tgcaggtcat
    28441 aatcatgttc aaattgagca acatgattat aaaaataaat caggagaaaa tgttatagtt
    28501 actgagccag gaaagcatgg agaatgtatt tctaagatta attttaaatt agaaaaaaac
    28561 aatgataaat ggaatatagt tgacaaatct agtaaattaa ttaaatttga aaaaaatcaa
    28621 gaaagtgata aatttggaga atttatttct tcaattaata aagtaagtaa agataagaaa
    28681 gaaataagat tatcagaagt aaagactcct gaatgggata aaatatatgt ttttaaatct
    28741 ggaacatctg cagatgatat atataaaacg ataggatata aatggagaaa tatatcatct
    28801 ccaaagggtg atgacatggt gcagattgtt gtaatgaata ataataagcc tgttagctat
    28861 gtatatggtg aagctgaagt tatgccaatt aatataaaat gtgatgaatc agagtataaa
    28921 gataatgttg taactattta tcctaataaa aatgataaat ttaaaattga aaaaggtaaa
    28981 caagaatata gtattaattt aaaacatata caatagaaac ttttatctta tttaagttta
    29041 gattgtactt aaaataaagt gtgtattatt ttaaaaattg atgataatta aaatcacaac
    29101 attaaggaat agtacacact ttttatgcta aaaaatagaa tgttaaaaaa ttaaaccttt
    29161 aaattaaagt acattgctta tattatccaa tattattttc aaaggttgtg taatattttt
    29221 atctttatgt aatattgcta acattttatt ttccatatct atcccatcta attcaatcaa
    29281 tcgcaatttt ttatgtaaga gtgaatctaa taataaattt tctggcaaaa tggttatacc
    29341 taaccctgct tctgcagctt taataagtgc aaaagaatta acactttccc aaacaggata
    29401 agctttttgg tttgaaagag ataatacact gtccaaagta tcgcgaatgg cacttccttg
    29461 ctcacgtaac aataacggaa acgtgcataa ctgtcgtaga gataaggctt gtttagataa
    29521 aggaaaagtt ggagcacatg caacacataa cttataggaa cctaataaaa ttgtacggaa
    29581 tgccccctgt ggttctatac cttcccaaaa gcatatatca gcttctccac tttttaaaat
    29641 atccatagct gaattagcac tttcgacacg tacattcact ttaagagatg ggaatgaatt
    29701 ttttagtcga cttaaaattg gtggaagtaa aaaagaggca attgtaatgc tagaaacaat
    29761 gtttataggt gtacattcct ctaaatgact gattcttttg tctaaattac gacaagcagt
    29821 taagatacta cgtgcttctt ctaaaagaga aatgccacag cgagtaagac atacccctcg
    29881 tggtagtctg tcaaataatg ctgtttcagc ttgttgttca agttcagcta tagcatgaga
    29941 tacagcagat tgtgtgatat gtaatttttt tgcagcacct gtaaaagtgc ctgtttgttc
    30001 aattgcttct aaaatctcta aatatcgtat gtttatcata ataaatatcc tcactagaca
    30061 tgaataaaat tgattaattc cattaaattc tatcatttta cagattgcaa agcaacaggt
    30121 atactgaata atatgaagtg aaaagaagga ggaatatttt gaatcaaagc agaaaaacat
    30181 atttttggct tttaattatt aacttgttta ttagcacatt cacttttggt ggagggtatg
    30241 tagttgtacc tatgattaga cgttattttg taaatcaaaa gcaattcttt acagaagatg
    30301 atttgatgag catggcagca gttgcacaat caactcctgg agctattgca attaatttat
    30361 ctgctttggc aggttatcga gtagctggta ctttaggggt tgtcattagt tgtatagcag
    30421 ctataatacc tccgctcgtt attcttggat tagtatcagc attttacacc acttttatat
    30481 ctaatgccat tgtaattgca gtattaaaag gaatgcaagc tggtgttgct gcactaattg
    30541 tggatttgat tattgacatg tgctctatga tattaaaaca acgttctcta tttttatcag
    30601 ctatgatacc tattgctttc tttgcaaact ttgtgatgag tataaatgta atttttatat
    30661 tatctgtttg ctgttttgta tgtgtaatgc aagttttcta caagaagagg gaaaataaat
    30721 gaatacaatt tggaatttat tttttatatt tttaaaaata ggcttgttta gtattggtgg
    30781 tggttatgca atcatacctt taatccaaga gaaagtagta aatcaaactg gatggataag
    30841 tgagaaaatg tttacagata ttataactat ttcacaaatg acaccagggc cactggctgt
    30901 taatacttct acttttgttg gacttcaaat tggagggttt tggggagcag ttgcagccac
    30961 tttaggttgt gtattatgtg gtgtagtaat atctctattt ttatataatt tttttcaaaa
    31021 gcatcagaac tctacttata tatttgaaat attaaatggt ctaaaatcag cttcattagg
    31081 tttaattatc tcagctgcgg ctacaattat aatccttgca ttatttggga cgaataaagt
    31141 gtacttcaat tctatatttg agatgctaaa ctggactgca ctaataatct tttttgttac
    31201 actactgata gtacgtaagt ggaaaattaa tcctattgtg attataataa taagtggtat
    31261 actgggaata tttttttatt tataagtttt tgctaagtaa aggatatcta tttttttata
    31321 tcttaaatcc aaatagccaa taaagtaagg ctatttggat ttttaataaa ttctgaaaag
    31381 ataatcagaa acccatataa catttaaatt atatcactgg ttttctacaa accaatcata
    31441 agcttcttgg caaaatttct cactatctat atagtaacta ttatgaacat tattaatttc
    31501 aacaagtttc tttttacatt ttagtgaatt ataatagcta tcaaaatatt gggttttaaa
    31561 atatctatca tgaagtgttt taaagacaag agtaggtatg ttgactccat cttttatctt
    31621 actatcttct gcaataaagc attctaacat tgtattaatt atctttgtac tatttttcca
    31681 aatatgagga tttttctttt caaatatttt ataaaattca ttatcataga cttcattata
    31741 acctttatat ttaggtggtt ttactgttat gtgaaaatta ggaagaaact taaagaaaaa
    31801 atataataat ttttatgttc tgttttatat attattcata ttttatcaca gataatatac
    31861 taaagttaat ttttttattg tacaactatt ataataattc ttctataata taagacattc
    31921 tataatttta tatttaagtt catgtgtatt taattgtttt tatataaaat gtccagtaat
    31981 actatttgga tttattttta tttcatcagg agttccagaa gcaacaattt cccctccatt
    32041 tattccactg tctggaccta tatcgataat ccaatcagaa gcacgtataa cctctgtatt
    32101 gtgttcaata attaaaagtg aatgctgttc atttacaagt tttctaaaag taattaacaa
    32161 attttctata tctttaaaat gaagtccagt agtaggctca tccaatatgt acagcattcg
    32221 ttttttagtt tttgtcccta attcttttgc taattttatc ctttgagatt ctcctccact
    32281 taatcctaat gtattttgtc caagctttag atatccaagt cccacttcat ctaataagga
    32341 gagttttcta tatatatcag agcaatcgct aaaaaatgtt tttgcttctg ctacttccat
    32401 gtcaagtata tctgcaattg tctttccctt gtattgtata gacagtattt ctttttgata
    32461 tctcttacct tgacatttac tacaagttac ccattggtct gccatatatt gcagaataat
    32521 ttttgtttta cctaatcctt gacagtttgg gcattgcccc tttttgctat taaagctaaa
    32581 atagctttca tccaataatt tttgttttgc ttgtggagtt tcagcaaata gagaacgaat
    32641 ttcatcaaaa atattgatat atgtagcagg tgtagaacgg ctagattttc caataggtgt
    32701 ttggtccatt aagataaaat catcaatagc atcaaatcca gtaaattcag tataattttt
    32761 atctgggata gatttttgtt tcaatttttc ttctagagca ggaagtaaac tgtgaaatac
    32821 aagagagctc ttaccagaac cactaacacc tgtaatagag cacatacagt ttaagggaat
    32881 ttctacatca atatttttta agttatttgc atggcatcct tttaagctta accatttact
    32941 tgggataatt ttattttgtg aacctgtttt attgtatgta cttaaatatt tacctgttat
    33001 agagtttgga ttttttataa tttcttgggt tgtaccttct gcaataatga accccccttt
    33061 tgtaccagca cttgggccta catcaataat ataatcagca gacagtattg tatctttatc
    33121 gtgttccaca actataacag tattaccctt atctcttagc tcttctatca ttttgatgat
    33181 taaattatgg tctcttgggt gtaaaccaat agatggttca tctaaaatgt aggttaagcc
    33241 tactaattca cttcctagct gacttgataa tcgtactctc tgcagttctc ctccagataa
    33301 agttggagct gttctatctg ttgatatata gctaagacct acctttaata gataactaac
    33361 tcgaattttt aattctgtta agatatcatt taccaaagag agctcatttt tttgaagttg
    33421 atttggtaat tcacacaacc aattccataa ctgttctatc gtcatttttg ttagttcagg
    33481 gaagcgataa cctttgattg tagtgaatct agcctcaatg cacagcaatt cgccaccaca
    33541 tgtattgcaa ggaattttgt tcatatattg atgcaaggta ttattttcag aactagattc
    33601 tctaaaaagt ctttgaatgt ggttgattgc tcctgaagca ggtcttctaa tctttgattc
    33661 tcgtcccttt gattcaaagc taaattcata tatcttatcg tctgtaccat ataagattgc
    33721 atctataaaa tctcttggta attcattcca aggtatatct aggtctatat ttttatcttt
    33781 tgctattgca tataattcac ctaccatcca attaccattt ggctttttcc ccctcaattt
    33841 tccaaaatag taagtggctc catctaatat tgaaatagtt gggtctttta caatcaattg
    33901 aggattaact tgataggtaa aacctaatcc attacaatcg tgacaagctc cagtatggga
    33961 gttagcattg aaggttgaag tatttatctt agaaaaaata gtttgacaat gtggacaata
    34021 attttttgta ttattctttt tttctaatgg aattttgcaa tatggacagt aactttttcc
    34081 gattgataca aataacattc ttaaataatc ccaaatgctt gtaagagtac caacagtaga
    34141 gcgaggattt tggcttcctt ttttctgttc gatagctata gtaggggtta atccctgaag
    34201 taaatcaaat tccggttttt gaagctgttc tgaaagtttt gctccattac ttagattttc
    34261 aaaataacgt ctttttcctt cttcataaat cgtatcgaaa gctaaagaag attttccact
    34321 accacttaca cctgtaataa cagttatttg attttttgga attgtaacct caacatcctt
    34381 taaattgttt tgccttcctc cttttaaata gatagagctt ttattatgac aaagtgaatt
    34441 tttaattgat ttatttattg attttgtaaa tttgaatttc ttttcgagtt caagaggatt
    34501 gcctaattct tttatttccg aaatgcctaa tatttttaac tgagaaaaga ttgatttcaa
    34561 ataatcttca ggcagttttt ctattaacaa attaaggtct tctattttta tatttccgat
    34621 tgataatatt tctgccattt catctaataa atttttttct gctcttgtta attgattgta
    34681 ttttttggaa cttaattctg tccataaatc ccatggaagc aattctaaac caatcatatt
    34741 attaaagtca tgtagaagat taccctttac agaatgccaa cctttccaat ttttattata
    34801 tccaaagtca tctggatgag catgtccttg ccgacatctt atccaggctt ctgaaccagt
    34861 taaaaaacca tctgtttttc cgacatcatg agtgtcaaaa ttaatttgaa aataattttt
    34921 ttgtatgtca tctatttgtg catctattct tatccatctc tttgttagag tatcatagta
    34981 ttctacaagc cagtggtctt cataagtaag ctcactttca aaataattag caaaacctgc
    35041 tctcattcta gcctcaaaac ctcgatatct taggagcgat acaagaagta aagaataatc
    35101 tctgcacatt ccaacaagcc tttgttttgg gtttcttgga atagttagtg ggttagaatt
    35161 tatttcaaat attcttttta acatttgagg aattgtacga agcaattcct catcagattg
    35221 ttgtttatta aaggatatac catataattt tccttggtct ccatgaatta ctaggctttg
    35281 aaccactttt aataactcaa ttggagtctt tggtaaatct tgatatttat tttcaaattt
    35341 tttaggattt gtgatgatac tttgattttt atagttattt attttcattt atatttcacc
    35401 tctctaaatg gatgatatac cctgacatgg tgtcatatgc aaggacaatt tagatttttt
    35461 ggccaaaatt taaaaataaa taaaaaatga tacagtgata aattgtaata caaatatagt
    35521 ttcaaattgc atataatata attatttaat aaaaccatta tctattgaat gttaatgaaa
    35581 tgttaagatg gcattcttga atattaagaa atagaatact ataatatagt gtgaaggatt
    35641 tttagtaaaa aaatttgtaa gtgttaatat acattaaaaa ataggtttat aatttggagg
    35701 aaaaaaatga aattatcaag gtataagaaa aatagtttag ttctgtctat aattttatgt
    35761 tcacttatta tttcaatcct tgtaattact gtttttaata ccgtaaaact acacaatata
    35821 tctaataaac aatctaaaaa ctatttaaat gatgtatcta cacagattgt aatgaatgtt
    35881 gattctaaaa tcaaatttat tttatccgac ttaagaataa tggcagattt tattaagcaa
    35941 tatgaaggtg atagtcgata tgagtattta tctaaaagaa agactagtta taagtattat
    36001 gatgttggaa taatagattt agaaggtaaa gctgagtttt taagtggaaa aaagtttgac
    36061 ttaaagaata ctgtttccta taaaaaagga attgaaggta aagaatactc agaagtaata
    36121 gagtcaatgg gttttgttct ttatagtgta cctatattta atgataatca tgaagtatct
    36181 agtattttaa taggtgtatc caataaagaa agtatgaatg agattcttaa tatagataac
    36241 tttaatggaa aaggaactat tgaaattatt aatagtaagg gaaaacctct tttcatagga
    36301 aaaaatactg aattaataaa ggaccttagc cataaatata agagttctgg agatgaacct
    36361 tgggcacaaa agatgcttga ggattttaaa aatgataaat cagggaatat tacaataacc
    36421 tcatcaaaag gtgtacaatg cttattaact tatcatccaa ttacaagagg tttaaatgat
    36481 tggcattttc ttctcatagt acctgaagat gctgtacttg gtgaacttaa taaattaaac
    36541 tcttttacaa taacaatgac atttataatt acatgtatta ttggaattat aacattaatt
    36601 ttatatatta taagaagaaa atatgtaaat caaatagaaa atattgcata tttagatagt
    36661 ataacaaatg gtataaattc aactaagttt agtatgctag tcaaaccttt aatttcagaa
    36721 tcacctgata gtacttatat gatgatagca atgaatataa aagattttaa gctaataaat
    36781 gattgtttcg gtagtgaaaa gggaaatatg acactaaaac atctttacaa tattttgtat
    36841 aaaaacattg atgaaaatga agagtttgtg tgccgtcatg atgcagacct tttctatttg
    36901 cttattaaga atagaccagt gttagaagcc ctggagttat tatataaaat agaagctgac
    36961 acaaattatt ttaataaaaa tagggaaaat ccatactttt taagactatc agtaggtatt
    37021 tacacaattg aaaatcataa tgaagattta attacaatac aagaccatgc aaatacagca
    37081 agaaaaagct ttaataaaac tcatcaaagt gatttttctt tttatagtga tatagaaaga
    37141 aaacgattaa tatctgaaaa ggaaatttca aatttaatgg aaaaagccct tgaaaataaa
    37201 gaatttttta tgtgcttaca acctaaaatt gatataaaaa gtggaaaaat tagtggagca
    37261 gagtctcttg tgcgttggaa aaattcagaa aagggcataa tttatccaag tgattttatt
    37321 cctcttttcg aaaaaagtgg atttatatgt aagttagatt tatatatact agaagaaaca
    37381 tgcaaattga tttctaagtg gataaaagaa ggtaaagaag tgttgagaat ttctgttaat
    37441 gtatcacgtc aacatctgaa tgataaattg tttttagaaa aatataagca tatatgcaat
    37501 aaatacaatg taccaacatg tctgatagat ttagaactta ctgaatctat tttcttagaa
    37561 aatccagaag ctatagatat aatagaggat attcattcta atggatttaa atgttctata
    37621 gacgattttg gatttgggta ctcatcatta gggatattaa aggattttaa agttgatata
    37681 attaagttag accgttcatt ctttgttagc aaaaataata tagaccgtgg taaggtagtt
    37741 attaagtcta ttattgaact tagcaaaagg ctaggtatga aagtaactgc tgaaggaatt
    37801 gaagaattag aacaagttga gttcctaaaa aatgtgggat gtgattatat tcaaggttat
    37861 gttttttcaa aaccattatt aatttctgac tttgaaaatt ttgcatatga aaataataaa
    37921 attaaaaata tccattatta aatgtacaag ataaaaaatt taggcaagtt atataattaa
    37981 aaatataact tgcctttttt atttatatat ttaataaaaa tatatttgca gattttgtaa
    38041 aaataatttt caaatgttgt aagttgtggc taaagcttta ttgtattatt ctgaatatag
    38101 aaagatagtt actgtcttag ataaaaactg aaacatacat aataatataa aatttgctat
    38161 attaataact ctaattattt tggaaggagg gaaaatatga taagtgggag aatgttactc
    38221 atcatgaatt ttctaaaagg aaaaaataag acaagttaca aggatattag caaacaactt
    38281 agtatagaag aaagaaaagt tagatatgat ataaataatt taaatattgt tttgcgttca
    38341 ttaaacaagt cagtaataca aaagatgaac aaaggtgtgt taattattcc tgatgacttc
    38401 caagtagtat gtataaataa taacgaatat gtattttcct taactgagcg agtatctatt
    38461 atgaaaatta tatcaatgtt taaaatagat aatctaaact tagaaaaatt aagtaaagaa
    38521 tttcaggttt ctagaagttc aattaaaaat gaccttaatg ttttgtcaag tgaattagaa
    38581 agaaaccaaa taactattac ttatgataaa tcttttaaaa taagtggaga agaagacatt
    38641 atatataagc aaagattgaa catattaaaa tcgtattctt atctttttgg taaggaaaaa
    38701 tctgatttaa gtgtatttga gaagtattta gtgaatgtaa ttgaaaaaat attcaaaggg
    38761 atttatttga ttgatatata taattggtct atgaatttat tattgcaaat gggatggact
    38821 ttaaatgatg aatcttttac atggtatgtg tcgaacattt tcctttttac atggtatata
    38881 cattcagatg aaaaacatcc tttagaatct gcttcattaa gtgagccgtg ttttgaaaat
    38941 aagtttgacc aagaatttga aacaattatt caaaaaaagt tgactaagga acaaattttt
    39001 ctgttaaaga gttttgtatt ttttacaaat aaatatgcaa gtttaaacga ggatatggat
    39061 ttgattagta cagaaattat agttcaagac ctaatttaca atatgtcaga aatattagag
    39121 attagcttta aagaagatat gattctttat aaaggattac tcaatcatat tgctccattg
    39181 atagagagaa tcaagcagaa tatacagatt tatgaggaat tacttcatgt aataccagat
    39241 aaatatcaat atgtacttga agctactaag gtttctattc aaaaaaacag agttttaagt
    39301 caaataaaga atgaaaatga aacaatactt ttggcaatac attttttagg aagtatacat
    39361 agaaatgaga ggaataacta caaaaatata ttgttagttt gtggatttgg atatggtgta
    39421 attgctatgt taaaggatag tttagtaaat gattaccaga taaatatagt agagagtatt
    39481 ccatcatata agttacaaga ttataaagaa tgggaaagta ttgacttcat tataacaaca
    39541 tcaaagatag taatagactt gcctaaaaca attattgaga taaatccatt tttacaagaa
    39601 gaggattatt tgaagtttga agaaaaaggg ataaaaagaa agaacgtctt aactaattat
    39661 atgtcaataa aaaaaaggtt ggacttttta gaagaagatg cccaaaaaag agtattaagt
    39721 attatatgtc aagaacttgg atacagtgat gagagaatat tatttagaca acttaattta
    39781 agtgatttaa tagggattga taccataagg ataattgatg gagatatgaa atgggaagat
    39841 gctgtaaaat ttagttcaaa catacttgct aattgtgatt ttgtatcagc acaatatgct
    39901 gagaatatta tagatatttt aaaaaatgtg ggtttttatg ctgtaaaaga taacgaattt
    39961 gctttactac atggcaatga tagttcatta gtcaaagtaa gttcgataag tttattaata
    40021 tgtaaagaag cagttagttt tggagataaa aaggttaaaa tcatattttg cctagcaagc
    40081 agagataaaa aagagcaaat tcctgcaata attaatctaa ctaaaatggt ttataagact
    40141 aattttattg ctatgttaga aagtgcaaag acaaaagaag aagcagaagt tttaattcat
    40201 aaatatgaga aggaggtcac tgtatgaata aattagttaa tgtaaaatgt tcatttaaaa
    40261 tacatgttaa ttcaaaagaa gaagccatta tatcgttagt tgatgtagtg tcaaaagaag
    40321 gatatttaat agataaaaat caatttttaa aagatgtttt aaagagagaa gaaacattgt
    40381 cgacttatat tggacatggt ataggtcttc ctcatagtca aagtgtaggt gttaaaaact
    40441 cttgtattac aataggaaag cttgatactc ctattgaatg gacagaagaa ggagaaaaag
    40501 tggatttaat atttttgata tcagtaacaa aagacaatga aaataatctt catttaaaga
    40561 ttttatcaaa attagcacgt ttattgatgc atgaaagttt tagaaatcaa attagatgtt
    40621 cagatgaaca aactgtttat aatcttataa aagaaaaaat agaggaggaa gattaaaatg
    40681 agaattttaa agtttttgca aaaacatctt atgacagcaa catcttatat gattccattt
    40741 gtggttgcag gaggaatatt atttgcttta tcagtaactt taagtggaca agctgctgtt
    40801 cctgaaacag gctggttggc taaactgaat caaattggag ctgctggctt ggctttattc
    40861 attcctatat taggtggata tatagcattt agtatggcag ataaaccagg attagctcca
    40921 ggtatgatag gtgcatattt agcaaaagaa gtaaatgcag gttttatagg tggtatcata
    40981 gcaggattta tagcaggatt tgttgtttta caattaaaga aaattaaatt agctcctaca
    41041 atgagaactt taggttctat atttatttat cctttagttg gtacattgat tacaggtggt
    41101 ataattgtat ttttaattgg agaacctata gcttctttta tgacatggat gacaaactgg
    41161 ttaaatggaa tgtctggtgt ttctaaaatt cctttaggtg gaatcttagg aggaatgata
    41221 gcctcagata tgggtggccc tataaataag gttgctgcta cttttgccca aactcaagtt
    41281 gacacacttc catatttaat gggtggagtt ggcgttgcaa tttgtatacc tccaattggt
    41341 ttaggattag ctacattatt gtttccaaaa aaatttagca aggaagaaag agattcaggt
    41401 aaagcttcac tattaatggg atgtgttgga attacagaag gagctattcc atttgcaaca
    41461 tcagatccag ttagagtaat accttgtatt atggctgggt cgattgtagg aaatattatg
    41521 gcttttttat taggatgttt aaatcatgca ccctggggag gtctgatagt gttacctgta
    41581 gttgacaata ggttggggta tattgcatca gtattaactg gagcagtagt tgttgcggta
    41641 ttaatgaaat tagtaaaaaa agacgtgaaa gaagatgaag aaattgaaga agatttagat
    41701 gaatcaattg aattaatatt tgaagaatta taaatataaa tggaaggaat ttgtataaaa
    41761 aagtaaatat aaaactataa atttaaagga gataagaata tgaaaattgt agcagtaaca
    41821 tcttgtccta gtggagtagc acatacttac atgtcggctg aatcacttga attatcagcc
    41881 aaaaaatttg gtatagaaat aaaggttgag actcaaggaa gctctggaat agataatgaa
    41941 ttgtcactta aagatattga tgaagcaact tgtgtaatat tgactaatga tgttgagatt
    42001 agaaatatgg aacgttttaa aggaaagaag gtagtaagaa tgagtgtatc tgagataatt
    42061 aagaaatctg atgcattaat taagaaaata aaagatactt ttcaataaaa attgatttat
    42121 tttacatata actcaaagat gtttttaaag aaatttttat taaaagcatc tttgaaaata
    42181 tcttaaaaat agaataaaag gagatatagt catgaaaaag ttaccaataa agactgtcgt
    42241 gcaaagatta ctaaacttac aagaagaggg gaaaagtgca actcttttag gaattggtcc
    42301 tatgtcacca aacttgttac aagcaagttt tgaactagca aaagatgatg attttccttt
    42361 aatgtttatc gcaagtagaa atcaagtaga tgcagatgaa ctaggtggcg gttatgtaaa
    42421 tggttggaat caggaaactt ttacaaaaga tattaagaag gttgccgaca aagttggatt
    42481 tgatggttta tattatttat gtagagacca tggtgggcca tggcaaagag ataaagagag
    42541 aaatgaccat ttgcctgttg atgaagcaat ggagttagga aaaaaatctt atttagcgga
    42601 cattgaagca ggttttgacc tattgatgat tgaccctaca aaagacccat ttgaaattgg
    42661 aaaagtaatt ccactagatg tagtactaga aagaactgta gaattaattg agtattgtga
    42721 aaacgagaga aaaagactta atctaccaga cataggttat gaggttggta cagaggaaac
    42781 caatggaggt ctgacttcaa ctgagacgta tgaaacattt ataactagac taaaggttga
    42841 actcgataat agaggtttgc caatgccaac atttatagtt ggtcaaactg gtactttaac
    42901 tagaaagaca gaacaagtag gaacttttaa ttttagaaat gcttatgatt tagctcaaat
    42961 ggctaagaaa tatggagttg gtttaaaaga gcataatggc gattacttag atgatgtaac
    43021 tttattagaa catataccat ctcaaattat tgctacaaat gtagctcctc aatatggtac
    43081 tgaagaaact agggcatatt taaaattagc agaagttgag tgtaaattag aaaaagaggg
    43141 tttagttgag aaaacttcta atatcagaca tgttcttctt gttaatgcaa ttgaatgtgg
    43201 acgttggaga aaatgggtag taggagaaca aaaaaatcta actactgaag agatatttaa
    43261 ggatgaagtt ttatctaatg aaatactaga tattgcagga cattatacat ttaataatga
    43321 tgatgttaaa aaagaaatag aagtgcttta tgataattta agtaagaata atattgatgg
    43381 tcaaagattt gttgtagacc atattaaacg tcctttgcgt gattatgcag aatgctataa
    43441 cttaaaaggt gttactacga ggattttaaa taaataatag gacttatagt ggtgagtatt
    43501 gttatactag gtatttatag ttataattat acttaccact attaaattta ggaggactaa
    43561 tattttgatt aatttaattt ttgatgtaga tgatacttta tataatcaat taacgccttt
    43621 ttacactgca tataataaag tgttttcttc aataaaggat atttctattg aagatttata
    43681 tatgagtagt agaaagtata gtgatgaggt atttcatatg actgaaaatg gagaaatgcc
    43741 aatcaaagaa atgcatattt ataggataat gaaagctttt gaggagttgg gaaattctat
    43801 tacagaaaaa gatgcacaaa gttttcaaga tgagtatata tatcaacaat cacaaattac
    43861 actaatacca gaagttgaga ggattttaaa tttttctaaa gaaagaaata ttaatctagg
    43921 aattataact aatggcccat caaaccatca aagaatgaag ttaaagcagt taaacattga
    43981 aaactgggtt gataaaagta atatttttat ttctagtgag gttggatttt ctaagccaga
    44041 tactaatatt tttagagtag ctgagaatgt tatgaattta gatagagaaa atacatatta
    44101 tgtgggtgat tcttatagaa atgatgtatt aggtgctaaa aaagctggtt ggaagagtat
    44161 atggttgaac catagaggac atgaggtaga agagttgttt tataaacctg attttgtaat
    44221 tttggaacat aaggatttga tatctttatt tataaaaata tgtagttata aaaatatgta
    44281 gtaaaaaaat gttaataata gaattgtata aaataattaa taataactat ataaaattat
    44341 atgtttagat tgtgagcaat ctattttatg ttaaaaatag attatttaga agaaggggaa
    44401 atcttaaaaa aatttgaata tccccttttt attatttaat taaaatgaaa tagttagaaa
    44461 tatattttat ttagtttagt caataacatt ttgggattct ttataactac atgttaactg
    44521 ttgaataggt ttatcatatg gtttaaccca tcctttatta atacttaatt ctgtaagtgc
    44581 tgtgtgacct tctaccattt gagttaatga agctgaatat atagctctta attcaggtgt
    44641 actacttgtc aatgcagaat taagatacat attggcagat gctttagcag atgaaagcat
    44701 acttaacatt attactttat cgtctatata agtattgttc ttaattagat tttctatcat
    44761 atttctcatg ttatctatac ctcctcagaa attagtattt cattttcaac tataaattgt
    44821 tgtatagctt taattcttcc ttctgttgct aatatactag attctgattg tctttttagg
    44881 tcttcatctg atattaacat atttattgct ctttgcataa gtaatccgtc agactccatt
    44941 tttataattg ctgctaaaga tattttttca gtttctgaaa gtattctcat ttaatcatct
    45001 cctatattgt aatttatgct ttaatagtat gtctttcttt tcaaggaaat aaccattaca
    45061 cttttttaca gtattttata ttctctttat atcaaaattt agtcgaccta attaatataa
    45121 ttttgtagga tatttatcat taaatatcta taaaataaat tatttgaata ttcataaagc
    45181 aaaatttatt tgtatgtact tcttcaagag tcaatatgag ttttttcaaa aaattaattt
    45241 ttatgataat cattaagggc tatatttttc ttaaagttgt gattatcatt attaatttac
    45301 cttagaggtt aattaggtgt ttaatcaaag ttaatgcctt tagaatacaa atgtattcta
    45361 aaggcatttt tactatgtat tttcatttat attactaaac attatctatt tttatataca
    45421 tttctatgta ttttattttc atcaaatcgg tctataaact catttttttc ttcactatat
    45481 ccaatagata taagtgcata aggaactaat tttaatggta agtctaaagt ttgtctaact
    45541 ttatttacca ctttattttt aggaaatact cctgcccaac aagcagctag accttgatta
    45601 gtaacttcta ataataaatt ttgtgtgcaa gcacctaaat cttgtatcca tttaccaggt
    45661 tttaagaatt tatctctatt tccaagcact actatacata atggtgcaaa ttctatatgc
    45721 tttgctctat gctgtgactt tgagagctgt ttcagcagtt ccttatcatc tactataata
    45781 aattcccaag gttgagcatt ttttgatgaa ggtgcttgca tagctgattt tagtatttca
    45841 gttattttat cttcttctat atttttatct atatatgttc ttattgaacg tctatttttt
    45901 ataacttcta acatacagat tctcctttaa ataaaattat ttctatttat ataatatcat
    45961 tactttaaat tttagctatt acttatataa agaataaact ttgtgttggt tttatctaag
    46021 taaaagtaac attcagaact aaaaaacaag actataaatc caaagtatta attaaaaaag
    46081 aagtaataaa aaataatata caagatttaa aaaagagtat tgggcttaaa atttaattag
    46141 aaaaccaatt ttgagataat ctgtttttta ttttaacaaa aaaacataaa taaagttcga
    46201 tacgtagcga atgaaaaaat tacaagaaat atttttttta aaaaatttca aaaaagagtt
    46261 gataatatat gtatattaat ataaaattta aggtgtataa ttcgtataat aatacgaatg
    46321 atttgtagtg taaaataact tataaacgga gaggggaagt ctaatgaaag tagcagtagt
    46381 tatgggttca aaatcagatt atccaaaatt agaagaaggg ataaaactgc ttgaaaaata
    46441 tggaatagaa gttgtagcaa gagcattatc agctcataga acaccagaac aactttcaat
    46501 attcctaaaa gaaatagaag atgatactga tgtaataata gcagcagcag gaaaagcagc
    46561 acacttacca ggtgtaatag cttcacaaac attaattcct gtaataggat tacctattaa
    46621 atcatcaact atggatggac tagactcact tttatcaata gttcaaatgc caaaaggaat
    46681 cccagttgca acagttacaa tagacttagg tttaaacgca gctttattgg cattacaaat
    46741 aatgacttta aaatacccaa aattaaaaga ggatttgaaa tcatatagag aagagatggc
    46801 acaaaaggtg ttagaagacg acaagaattt aagggggtag cagaaatgtt attatatgaa
    46861 ggaaaagcaa aacaagtata ttctacagat aatgaaaatg aatatgttgt ttattataaa
    46921 gatgatgcaa cagcatttaa tggagaaaaa aaagcagaaa tatcttcaaa aggtatacta
    46981 aataataaga tatcaacaat aatctttgaa atgttaaaag aaaataatat aaatactcac
    47041 tttataaaaa gtttatcaga tagagaaatg ttagttaaaa aagtagagat tttaccatta
    47101 gaagtaatag taagaaatat tgctgctggt tctatctgta aaagagttgg tcttgaagaa
    47161 ggtgtagttt tcgatgagcc aatttttgaa ataagctaca aaaatgatgc ttatggggac
    47221 ccaatgctaa atgatgatta tgcagtggca atgaagttag caactagaga agagttaaaa
    47281 ttcttaagag aagaaacttt aaaaataaat gagttactaa aagcattttt cttaaaatta
    47341 aacctaaagt tagttgactt taaaatagaa tttggtaagg attctgaagg aaatataata
    47401 ttggcagatg aagtatcacc agacacttgt agactttggg atgtaaatac aaatgaaaag
    47461 ttagataagg acagatttag aaaagacctt ggagaccttg tagaaggtta tacagaagta
    47521 ctttcaagaa tgaataataa ataggaggca aatgtatgtg tggagtttta gggatttact
    47581 ctaataaaga tgtaactaaa gaactatatt attctttata ttctatgcaa cacagggggc
    47641 aagaaagctg tggtttagct cttttagatg atggagaaat taagtacaaa aaagatatgg
    47701 gattagttgg agatgttttt aaggaaaatg aattatctaa gttaaaagga aatataggga
    47761 ttggacatgt tagatattca acagctggag gaagtcatgt ttctaattgt caacctttag
    47821 ttggaagttg tagaaaaaga caattagcca tagctcataa tggaaatcta gttaatgcaa
    47881 attatttaaa agatatgcta gaagaagatg gatatatgtt ccaaactaat tcagatacag
    47941 aagtgatttt atatatactt gctagatatt acaaagggga tatagtagaa agtttaaaag
    48001 tgacaatgga ttatataaaa ggtgcatatg ctcttgtaat aatgagtcaa gaagaattgg
    48061 ttgcagttag agacccacat ggattcagac ctcttgtact tggtaaaaag ggtgatgaat
    48121 atatatttgc atcagaaaat tgtgcaatag atatacttgg cggagaagtt ataagagatg
    48181 tagagccagg agagataata gtagtcaaag atggagaact aaaatcatat ttttattcag
    48241 aaaattataa accagttaag aaaagttgta tatttgaaca catatacttt gcaagaaatg
    48301 atgcaactat agacaatgta aatgcatatg aatttagaat taaatgtggt gagagattag
    48361 ctcagaatga aactgtaaag gcagatatgg tagttccagt tccagattca ggttggccag
    48421 gagctattgg ttatgcaaat gctagtggat taaaaataag tgaaggctta gttaaaaata
    48481 gatatgtagg aagaacattt ataaaaccta cacaagaaga aagagaaata gcagtaaaaa
    48541 taaaattaaa tcctctttca acaataataa aaggaaaatc tataatttta gtagacgact
    48601 caattgtaag aggaactaca tctaaacaat tagtaaaatc tttaagagaa gcaggagcaa
    48661 aagaaattca tcttagaata acttctcctc cagtagctta ctcttgttac tatggaatag
    48721 atactccaaa tcgctctaaa ttaattgcat caagtaacaa cgtagaagaa atgagagaat
    48781 atattggatg tgatagttta aaattcctag atatagaagg aatgttagat gcaacagaac
    48841 ataagtctac tttctgtaaa gcttgttttg atggagaata cccagttaag aaaattgata
    48901 aggaggaact actttcatgt taacatataa agaatcaggt gtagatatag atgaaggaaa
    48961 tagagcagta gaccttataa aaggtaaaat aaaaggaact tatgatggaa atgtagttgg
    49021 agatttaggt aacttcagtg gattatacag tttaaaagat tttgtaggta tgaaagaacc
    49081 agttttgctt gcatctacag atggtgttgg gactaagtta aaaatagctc agatgatgga
    49141 caagcatgat actgttggaa tagatttagt tgcaatgtgc gtgaatgatt taatttgtca
    49201 aggagcaaaa ccattattct tccttgacta tatagctctt gggaaattag tgcctgagca
    49261 tatagaaaaa atagttggtg gaattgcaga tggttgcaaa atgtctggtt gtgcactaat
    49321 tggtggagaa actgctgaaa tgcctggaat gtatggagaa gatgactatg atttagcagg
    49381 attttctgtg ggaatagcag acaaagaaaa gattgtttct ggaaacaatg ttaaatcagg
    49441 agatgtattg gttggtatat cttcaagtgg agtacacagc aatggatttt cttttataag
    49501 aaagatattt ttagaaactt acaattataa aatggaacaa tatgttgaag aactaggaat
    49561 gacagttgga gaagcacttt taacaccaac taaaatatat gttaaattag ctttagatgt
    49621 attagctaaa catgacataa aagctatagc tcacataact ggtggagggc ttatagaaaa
    49681 tataacaaga gttataccta aagggttagg actagatatc aataaaaaat cttgggagaa
    49741 gcctcctata tttaaaatga tagaaggttt taatgctgtt gatgagagag aacttcataa
    49801 gagttttaac atgggtattg ggctagtttt aatagtagat aaagaaaatg ctgatgatgt
    49861 tgtaaacttt ataaataata gagaaaatga caatgctgat tatgtagata aaaaatatag
    49921 tgaattatta gaagataaag cttatataat tggtgaagta gtagatagtc atgaaggtgt
    49981 agaattatgc taaatatagg agttttaata tctggtgggg gaacaaattt acaagcagtt
    50041 atagatggta ctgagtctgg agaaataaaa ggtcaggtta aagtagttat ctcaagtaag
    50101 caaggtgctt atgggctaga gagagctaaa aatcataaca taaaagcaat ttgtgaaact
    50161 gatgaagaca agataataga aattcttaag gaaaataaaa tagatttagt agttttggct
    50221 ggttatttaa aaataatcag cccaaagctg gttaatgagt ttagaaacaa aatgataaac
    50281 atacatccat cattaattcc ttcattttgt ggagctggat tctatggaga aaaagttcat
    50341 caaggtgtta tagattacgg agctaaagta actggtgcta cagttcattt tgttgatgaa
    50401 ggtgctgata ctggaccaat cataatgcaa gatgtagtta aggtaaatca agatgatgat
    50461 gcaaagacac ttgcaaaaag agtgttagaa gttgaacata ggattttaaa agaaagtata
    50521 agtctttttt gtgaaaataa aataaaactt caaggtagga gagtgtttat aaatgagtaa
    50581 gagagcttta ataagtgtaa ctgacaagac tggtgtagtg gaatttgcta aagagttaaa
    50641 taaattagat tatgaaatta tatctacagg aaatacattt aaaacattaa aagaaaatgg
    50701 tgttaatgta atgcaagttg aagatgttac aaattttcca gaaatattag atggaagagt
    50761 aaaaacatta aatccatata tacatggtgg tatactttat aaaagagata aagaatctca
    50821 tgtagaaact gtaaatgaac acaaaataca ttcaatagat ttagtagctg taaatctata
    50881 tgattttgaa ggaactttaa aagctggaaa aagtcatgat gaaataattg aaaatataga
    50941 tattggtgga ccatctatga tacgttctgc tgccaaaaac tataaggatg taatagtagt
    51001 agtagatata aaagattatg attctataat agaaaaatta aaaacagata caatgacatt
    51061 agaagacaga aaaaaattat catataaagc tttctcaact acaggaagat atgatgcgtt
    51121 aatatctagt tattttgctg gagaagttgg agatacgtat cctgacatat taaatttaac
    51181 tttccaaaaa gaacaaactt taagatatgg agaaaatcca catcaaaatg gtttcttata
    51241 ttcacagtca aatgcaaaaa atccaatctt aaattatgag caattaggcg gtaaagaact
    51301 ttcttttaac aacttaaatg atttacatgg atgtttagaa gtaatgagag aatttaaaga
    51361 tagtgaagaa gtagtttcag tagctataaa acatgctaat tcatgtgggg tagggttagg
    51421 aaaagatgct tttgaagcat atacaaaatg ttatgaagcg gataaggttt caatatttgg
    51481 aggaatagta ggaataactt ctactataga taaagctaca gcagagaaat taaatgaaat
    51541 attcttagaa atagtagtag catatgattt tgaaccagaa gctttagaga tacttaaaca
    51601 aaagaaaaat ctaagaatat taaaattagc taaaatagaa aatagtttac aaccatatga
    51661 aatgaaatat ttagatggaa aattattgat acaagataga aacaatatat tagcagaaaa
    51721 aagtgaaaat gtaacaaagg aaaaaccaac agatgcacaa cttaaggaca tggaatttgg
    51781 tatgagagta gtaaaaaata tgaaatcaaa tgcaattgca atagtaaaaa atggacaaac
    51841 tctagcttta ggttgtggac aaacatcaag aatttgggca ttaaaaaatg ctcttgaaaa
    51901 taacaaagat aaagatttta caggagctgt tttagcgtca gacgcatttt tcccatttga
    51961 tgattgtgta acattagctc atgaatatgg aatatcagca gtagttcaac caggtggctc
    52021 tataaaagat aaagattcaa tagaagcttg tgacaagtat gatatggtta tggtatttac
    52081 aggaataaga cacttcaaac actaaggagg atattatgaa gatattggtt gtaggcggtg
    52141 gcggaagaga gcatgctata tgctggaaac taagcaaaga aaaaaatgta gaaaaaattt
    52201 attgtgcacc aggtaatgca ggaattgcaa acgtagcaga atgtgtaaat ataggtgata
    52261 ctaatataga agaactttta aaatttgcca aagaaaatga aataggtctg acaatagtag
    52321 gaccagaagt accactagtt atgggaatag tagatgagtt tgaaaaagaa ggattaagag
    52381 tatttggacc taataaaaaa tgtgctcaac ttgaaggaag taaagctttt tcaaaagaat
    52441 ttatgataaa acataacata cctactgcaa aatataaaga atatacaaac ttagaagaag
    52501 ctatatcaga aatagatagt tttggatatc cagtagtaat aaaagcggac ggattagctg
    52561 ctggaaaagg tgttgtaata cctgaaaata gagaagatgc tatagcaact ttaaaagaaa
    52621 tgatgtcaga taagaagttt ggagctgctg gagataaaat agtaattgaa gagtttttaa
    52681 aaggaataga aacttcaatt ctagcatttg tagataatga tacaatagtt cctatggcaa
    52741 gtgcaaaaga ccataaaaaa gtaaacaact atgagcaagg tccaaacact gggggaatgg
    52801 gaactttttc acctagtgaa atatatacag aagaattagc aaataaagta aaagagacag
    52861 tattagaaaa aactttagaa ggcttcaaaa aagatggact taattttaaa ggtattttat
    52921 ttgttggtct tatgataact gaagatggag aaaaagtact tgaatataat gtaagatttg
    52981 gagaccctga gacacaatct gttttattta gattagaaac agatttacat gagataatgg
    53041 aagctatact agataataag ttaaaagata tagaaataaa ctatagtgat gatgaagctg
    53101 tgtgtgttat gcttacatca ggagggtatc cagacagtta tgaaaaaggg aaaattataa
    53161 ctggtcttga aaacttggat gatgatatag tagtattcca tagtggcact aagatgtttg
    53221 atggtaattt ggttaccaat ggaggaagag taattggtat aactgcaaag tcaactacag
    53281 ttaaggatgc agcagaaaaa gtttatgaaa acataaagaa gataaacttt gaaggaatgc
    53341 actatagaac agacatagga agataatttt tctaaaatgg aggataaggt atgttgaata
    53401 ctgaaaataa agactcaatg gttagaagag tattagttga aaaaagagaa ggttttgacc
    53461 ttgaggctaa agctttaaaa aaagacttag tagaaagttt acacatagat aatatagaaa
    53521 atttaagaat attaaataga tatgatgtag aaggaatatc agaagaagtt tatgaaaatg
    53581 cagcaaagac aatattctct gaaccaaatt tagatgtagt atattatgaa gaaataccaa
    53641 aattaaatga tgaaagagta tttgcaatag aatttcttcc tggtcaatac gaccaaagag
    53701 gtgattgggc agctcaatgt gtacaaattg taaatcaagg gataagacct gctattaata
    53761 cagcaaaagt ttatatatta tcaggcaaaa taactgatga agagttttct aaaataaaag
    53821 attactgtat aaatccagta gatagtagag aagcatcttt agaaaaacca gaaacgttaa
    53881 aaatggaaac tgaaatacca acaacagttg aagtattaga tggatttata gatttagatg
    53941 aaaatggatt aaaaacattt gtaagtgaaa aaggattagc aatgacttta ggagacttac
    54001 aacacgttca aaaatacttt aaagatacag aaaagagaaa tccaactata acagaaataa
    54061 aagtattaga tacttattgg tctgatcatt gtagacatac tacttttatg actgaaatag
    54121 aaaatgtaaa gatagaagat ggtaagttta atgatatagt aaaagaagcg tatcaaatgt
    54181 atcttaactc aagagacaat gtatatgtga acagacataa agatatatgt ttaatggata
    54241 tagccactgt tgcagtaaaa gaattaaaga aaaatgggaa attaaatgac ctagatgaaa
    54301 gtgaagaaat aaatgcatgt agtataaacg tagatgttga agttgatggt aagatggaaa
    54361 aatatctagt aatgtttaaa aatgaaactc acaaccatcc aactgaaata gaaccatttg
    54421 gtggagcagc tacttgttta ggaggagcta taagagaccc attatcagga agaagttatg
    54481 tttatcaagc aatgcgtgtt acaggaagtg ctgacccaag aacgacttta gaagatactt
    54541 tacctggaaa acttatgcaa agaaaaataa ctacagaagc tgctcatgga tatagttcat
    54601 atggtaacca aataggtctt acaactgggc aagttgcaga agtatatgat gaaaactttg
    54661 ttgcaaagag aatggaaata ggtgcagtta tagcagcagc accaaaagag aatgtagtaa
    54721 gagaaagacc tgaagcagga gatgtaatcg ttcttcttgg aggaaaaaca ggaagagatg
    54781 gctgtggagg agcaactggt tcatctaaag aacatagtga agagtcaatc ttaacttgta
    54841 gtgcagaagt tcaaaaagga gatgctccta acgaaagaaa aatacaaaga ttctttagaa
    54901 ataaagaagt tgctcaaatg ataaaaagat gtaatgattt tggagcaggt ggagtttgtg
    54961 ttgctatagg agagattgct gatagcttag atataaactt agacttagtt cctaaaaaat
    55021 atgatggact tgatggaact gagcttgcaa tttcagagtc acaagagcgt atggcagtag
    55081 ccataaaaaa agaaaataaa gataaattta tacaattagc agtagaagaa aacttagaag
    55141 ctacacatgt agctacagta acagatacag gatatttaag aatgttctgg aatggaaaag
    55201 caattgtaga tataaataga gagtttttag atacaaacgg agtaaaacaa actactgatg
    55261 ttcatgttac taaagtagat gaagaaaata cattctttag ctctaatgaa atagtaaaag
    55321 atgttaagtg tgcaagtatg aaagacaagt ttacaaaagt actatctgac cttaatgttt
    55381 gttctcaaaa aggattagta gagatgtttg ataatacaat tggtggtaat acagtcctta
    55441 tgccatttgg aggaaagtat caagcgactc caacacaagg tatggttgct aagataccag
    55501 tacttggagg agaaactaat acttctacaa taatgactta tggatacaat ccaaaggtag
    55561 gaaaatggag cccattccat ggtgccttat atgcagtagt agaatctgtg tgtaaattgg
    55621 ttgctattgg aggaaattat agtacaacaa gacttacatt ccaagagtat tttgaaaaac
    55681 ttggtaacaa tccagaaaaa tggggtaaac cattctcagc acttttagga gcattttatg
    55741 cacaaagtaa atttgagata cctgctatag gtggaaaaga cagtatgtct ggtacattta
    55801 aagatattga agttccacca acattagttt catttgctgt tgacacagta gatgcgaaga
    55861 aagtagtatc tccagaattt aaaaaagcag actcaaaagt agtaatgtta tgtgtaaaca
    55921 aggcagaaaa tgatgttgta gattttgagg aattaaagag aaacttagat aaagtaagag
    55981 aacttataca tggaaataaa gttttatcaa catatgcatt aggatttgca ggtgtaggtg
    56041 aagcaataag taaaatggct tttggtaata aaataggatt taagtttagt gaagaggctg
    56101 aaaaagcatt tacagatgat aaattatttg aggctagtta tggaaatata gtacttgaat
    56161 tagcaaatga tgatttaagt atgttagaag gatacaatta tgttgtgctt ggaagtacag
    56221 ttaaagaagc tagtatattt atcaaaggtg aagaacttgc attagatgaa ttatataaag
    56281 cacattgtag tactttagag cctatattcc caactaaaac agaagaagta aaatcaaaga
    56341 tagaaactat aagttatata tcacaaggag aagctaaaaa atcatcatta agtatagcta
    56401 ctccaagagt atttatacca gcattccctg gaacaaactg tgaatatgac tctgcaagag
    56461 catttgaaag agcaggagct aatgcaagta taagagtatt taagaattta acttataaag
    56521 acatagaaga ttctatagat acaatagtaa acgaaataaa atcttctcaa ataataatgt
    56581 taccaggtgg atttagtgct ggtgatgagc cagatggttc tggtaaattt atagctactg
    56641 tatttagaaa tcctagagtg caagaagcta taaatgaatt tttaactcaa aaagatggac
    56701 taatgttagg tatctgtaat ggattccaag tattaattaa attagggtta gttccatatg
    56761 gagaaataag agttccaagt gaaagtgctc caacacttac ttacaataat attggtcgcc
    56821 atcaagctaa gattgctaga actagaattt catctaataa atctccttgg ttagctcaaa
    56881 ctaatgttgg agatattcat aatatagcaa tttctcatgg agaaggtaag tttgttgcca
    56941 gtgaagatgt tatgagagaa ttaattgcta atggacaagt agcaactcaa tatgttgact
    57001 ttaataatga agctacttat gatattgagt ttaatccaaa tggttcattc tatgctgttg
    57061 aaggtataac tagtgctgat ggtagagtgt ttggtaaaat gggacattct gaaagaattg
    57121 gtgaggaagt atataagaat attataggtg aaaaagacca aaagatattt gaatctggag
    57181 ttaaatattt tagataaagc tttgatgttt aattgatagc tagaatatat aatttagata
    57241 tttagattta aagagatgta ataatatgat gataatcatt taatattaca tctctttttt
    57301 atttatctaa gaaataataa gttcaaccta agttaaaata caagctgtgt tataattaat
    57361 tattaagtaa aattgaatat ttattggagg gcaacataat taatgaaaat attaataaca
    57421 gggtctaatg gtcaattagg aaaagaattg gttaaccaat tagaagcaat taatcaaagt
    57481 ataaaccaac caaaatatgt tatattagct acaacaagaa gtgatttgga tatatccaat
    57541 cagactaatg tagataattt tattttgcat aataaacctg atgtagttgt aaactgtgca
    57601 gcatatacaa aggttgatgc atgtgaagat aatatcgaaa ttgcatacaa aataaatgct
    57661 ttaggagtga gaaatctagc aatagcatca gaaaaagtta atgctaagct tattcacata
    57721 tcaactgact atgtattcaa tggtttttct aaatatccat atagggaaga caataaaact
    57781 gaacccaatt ctgtatatgg aaaaagtaaa cttatgggag aaaaatttgt tgaacaattc
    57841 tcacataagt actttatatt gagaacagca tggttatatg gtaatgggaa taattttgta
    57901 aagaccatga taaaactttc tttagaaaat aaagaggtta atgtggttaa tgaccagttt
    57961 ggaactccaa caagtacagt agacttagca aaaattataa taagaatcat ggaaacagaa
    58021 cattatggag tttatcatgc tacatgtgaa ggtgagtgta gttggtatga ttttgccaaa
    58081 aagatattta aactaaaaaa tatagatata aaagtaaatc caattaagag taacgaattt
    58141 aaatgtaagg ctccaagacc actatattct gtattagata attttatgtt gaagttaatt
    58201 ggtttaaatt cttttagaaa gtgggaagaa tccattgaag aatatttaga aagatatgat
    58261 ataaatgaaa agtaaatgta ttattttgag aaaagaaaat aagatattat aaaaaatata
    58321 aatatttctt ttatttatcc gataatcata atatgacaaa aacaaattta aggatggaaa
    58381 aagatgaaag gaattatttt agcaggaggg tctggtacaa gactatatcc tataacaaaa
    58441 tgtatatcaa aacaaacact tccaatatac gataaaccaa tgatatatta ccctatgtcg
    58501 gttcttatgt tggcagggat acaagatata ttaataatat ctacaccaag agatataaaa
    58561 gcatttgaag aattatttgg agatggaagt tcactaggga ttaacataac atatgcaatt
    58621 caggagaaag caaatggaat tgcagaagcg tttataatag gagaagaatt tataggaaat
    58681 gacagagtgt ctcttgtatt aggggataat atattttatg gatatggatt tagtgaaagg
    58741 ttggagaatg cagttaaaag aaaaggagca actatatttg ggtatcatgt aagtaatcca
    58801 aattcgtttg gagttgtaga atttgataag gattttaatg tattgtcaat agaagaaaaa
    58861 cctacagttc ctaaatctaa ttatgctata ccaggattat atttttatga taatgatgtt
    58921 gtagagatag caaagctagt tgaaccgtca gatagaggtg aattagaaat aacctctata
    58981 aacagtgaat atctaaaacg aggaaaacta aaagtggaac ttctaggaag aggtatggct
    59041 tggcttgata ctggtacaca taaaggtcta ttagatgctt ctaattatgt tgaagtggtt
    59101 caaacaagac agggattgta tatagcatgt ttagaagaga ttgcatatac aaaaggatat
    59161 ataagtagag aacaattaat gaaactagcc aagccactga taaaaactga atatggtcaa
    59221 tatctattaa aattggctag tgaagatatg taatatggta gataaaattt attataaatt
    59281 ttattaaggg agtttattat aatgggaaaa atcaatgtta taaagactaa aatagaagga
    59341 ttatatatag tcgaaccaaa tgtattttat gatgaaagag gatattttat ggaaagctat
    59401 aataaaaagg attttataga gcagggtctt gatatgtgtt ttattcaaga taatgagtct
    59461 agctcaagaa aaggcgtttt aagaggaatg cattttcagg ttaaatatcc tcaaggaaaa
    59521 ttagtcagag ttacagaggg agaagttttc gatgttgcag ttgatttaag gaaagattca
    59581 caaacatttg ggaaatggga aggtgtaata ttgtctgata agaataaaaa acaattttat
    59641 attccagaag ggtttgcaca tggattttta gttttatcag aaaaagcagt atttagttac
    59701 aaatgtacaa attattatta cccagaattt gagaatggtt ttatatggaa tgataagcaa
    59761 atatctattg agtggccttt aaaaggaatt gaagaaatat ttttatcaga aaaggacaaa
    59821 aaacaaaaaa gttttaaaga tatttgtaca agacgttaat aaaaatttat tagaattaac
    59881 atttaggaga aaaaatatga aaaaaatact gataactgga ggagcaggat ttattgggag
    59941 taattttata cattatatgc ttaacaagta tgtagattat tttattgtta acctagactt
    60001 gttgacttat gctggtaatt tagagacatt aatagaaata caagacaatt ccaattataa
    60061 atttgttaaa ggagatattt caaataggga gcaggtgtat aagctatttg aagaagaaag
    60121 gtttgatata gttgttaact ttgccgctga atctcatgta gataggtcag ttgagaatcc
    60181 tgatttattt attaaaacaa acattatagg aacacaagta cttttagatg catctataaa
    60241 atatggagtt aaaaggtatc atcaaatatc tacagacgaa gtgtatggtg atttaccaat
    60301 tgatagaaag gatttattct ttacagaaca atcttctatc aatccaagtt ctccatattc
    60361 agcttctaaa gcatcagcag atttattggt aaattcttat tatagaacat atggattgtt
    60421 aacaactata tctaggtgtt ccaacaatta tgggctatat cattttcctg aaaaattgat
    60481 tcctcttatg attttaaatg ctttggaaaa caaacaattg ccagtatatg ggaatgggga
    60541 aaatgtaaga gattggttac atgtatatga ccattgtact gctatagact taataataca
    60601 taaaggaaat ataggggaga tttacaatat aggaggtcat aatgaaagaa gtaatctaga
    60661 agtagtaaaa gtgatattaa atcttttagg taaatctgaa gagttaatat cctatgtaaa
    60721 tgatagacct ggtcatgatt taagatatgc tattgatgca actaaaatag agaatgagtt
    60781 aggttggaag gcaaaatatg actttgattt gggtataaaa gaaactgtta agtggtatat
    60841 agaaaatgaa tcttggtgga aagctgtttt atcagtataa ctttaaaatt tagaaagggg
    60901 cgttttatat tgaatgagat aagttcacta attaatatat tagcattgac tcaattatct
    60961 ggtagtaata ctacaaatca atgtaattgt acatataata attcatctgg atttgatatg
    61021 attatgatga ccttgttaaa agcattatca caaaataatc aacatacttc taatagctac
    61081 aatttattgt ctaattctga gaatttattt aatgaacttg ataatgtagt aaataatacc
    61141 actagtagat ttatagatgt tgatacaaaa gataaaaatg taaagttgag aattgaaaat
    61201 gctgtagatc aagcatcaaa aaaatataac gtagattcaa atcttataaa agcaataatt
    61261 aaagttgaat cagattttaa tccaaatacc gtttcttcta ctggtgctaa gggtcttatg
    61321 caacttatgc cagaaaactg cagagactta ggagttacag acccttttaa tatagaacag
    61381 aatatagatg ctggaactag acatattaag gaatacattg atatgtttgg agggagcata
    61441 gaaatgggat tgatggcata taatggtggt cctggaagaa tgaaaagtag aggagtagaa
    61501 tcaatctctg acctgtataa aatgccaaaa gaaacacaaa attatattcc taaggttatg
    61561 aaatattata gagggtaggc tttattatgg gaataaatat agataagcta aacataagtc
    61621 caagtataaa tatggaggtt aaaaaactaa aagagagtaa taaattttct gacaaattaa
    61681 actctttaaa acaatcttct tcattggaag atataaaacg aaatttggaa agtgtaaaaa
    61741 aggcaggaga aaggcttgta cttactcaaa actatggcga tattagtata tataaaaatg
    61801 cagtaaaaga atacctaaaa tctgttgtag ataatatgta ttcacttgat aaaaatagta
    61861 gcttttggga gcatcagtat tataagaatg taaagattgt tgatagtaaa cttgaggata
    61921 tgactagtaa gttaattagt gaagaaaaag aaaacataga tattgtttct actgttgaca
    61981 tgatacaagg cttattgata gatatgtata gataaggtta tacaaggaga aaaattttat
    62041 gaatagcaaa aaagaggata tatatgaatt gaattttggg caattaatag aagaaaaatc
    62101 tggtgttata gaagaaaatc aaaatgtttt taatgcaaaa gtaaatgtag ctgtttctat
    62161 aggaaataca acagaaacaa taaaaaatat attagattta aaagaaggct ctataataaa
    62221 attaagtaag aatgtggatg aaaaattaga tatatatgca aacgatagat tgtttgctta
    62281 tggagaaagc attattacaa atgagaggtt atctgtaaga ttatctaaaa tagaagatcc
    62341 agatgtaaat tagcatttta aatagtatcc ctttaggcta ttctgtatga gtttagctaa
    62401 ggggatattt ttataaaaaa tttaaaatat gtgtgaatta tataaatatt tttattttat
    62461 gagcgataac taaattataa gtatgtaatt ttggaggtga aactatggat attaaaaatg
    62521 taagttcaaa cattgtagtg aataattatt taaagaatac atctggtata aaaaaagtgg
    62581 aagaggttaa aaaaattaat aaaacagaca agatagagtt atctaaattt gcaaaggaaa
    62641 tggcaagtgt gtcaaatatg agtgatgaga atattagaaa taaaaaagtt gatgaaataa
    62701 aaaatgcatt agaaaatgga acttacaaaa taaaaccaaa agaaattgcg aaaaaaatga
    62761 tagaggatat gcgaggataa agtcatgatt acagacttaa aagttataat atatgaagaa
    62821 aaaaatataa ttagtaagat gatggaatta ttaaaagaac aatttgatta tatagttgaa
    62881 aaagacttag aaaatttaaa tatgttaaat cctaaactag aagatattag cagggagctt
    62941 gcatccatag aaataaagag aagacaactt tttggtgatg atatttcaat gagtgaagtt
    63001 gttgaaaata gtaatgatgg atacttaaaa gaaatatata cagacttgaa acacattctt
    63061 gctttagcta aaaatcaaca agaatcaaat gattctctaa taaagaaaga acttattttt
    63121 acgaaaaaaa tgattaattt cataaaacca gctgataaga aaactacaac ttataattct
    63181 tatggaaata ttagaaaata aatattagga ggagaaatat ggcaggatta ttttccattt
    63241 taaatacagc taattcagga atgaatgcgc accaaaaagc aatacagaca atttctcata
    63301 atataagtaa tctagataca gacggttatt ctaggcaaag gaccgagttt gccacaaata
    63361 gtccaatgta tatgcctagt ttgagtaatt caattgcaag aggacaatta gggactggag
    63421 tacatgtaac ggatgtaaca agagctagaa atagttttta tgattaccaa tttagggcag
    63481 aagctcataa gtatggtaag acagtttcaa aatatgatta ttacaatact atagaaacta
    63541 tactaaatga accatcagac tatggaatat ctgcaggtat agatgatttt tttaaaggat
    63601 gggagtcatt atcaaaggat ataaatagtg acagtaaaaa aagtctagtg gtagaaaacg
    63661 catctaattt agcaacttta ataagtgaaa gttataagaa gcttgataag ttaaaagaag
    63721 atatagattc taatatagac actgagataa aacaaataaa tgacatgtta aaaagtcttg
    63781 aagatttaaa taaatctatt gatattatat ctggttctgg ctctacacca aatgatttac
    63841 ttgatgaaag agatagaatt ttagataact tgagttttaa actagacttt gaaaatagtg
    63901 atgttaaaaa tatgcttgca gatggtaaat tagaacttaa tgaattaaaa aatccagatg
    63961 gaacttggaa aacaggtata tcagggacct tacaaggctt gtttgaaatg catggtaaga
    64021 ttgatacata taaaagcgat ttaaaagatg tatctgatgg attagctaaa cagataaatg
    64081 atgtttataa tacgtctgca ggaataacag taacagattt ttttataact tcaaatgtag
    64141 atggtgaaga tattataaag ataaatccag ctataaagag taacccaaat gaattaaagc
    64201 ttactgctga tgaagcttca aaaattgcta agttaaagga tgaaaaaata gatattgggg
    64261 tggctggtgg aaaagtaagt acaataagtg accactataa agcctttgca gaatcaatcg
    64321 gtttagattc tcaaaaagta aatcaagatg aagcaaatca acgtaaaatt ataaataata
    64381 ttgataactc aagaatgagt gtttcaggtg tatctttgga tgaagaaatg acagagctta
    64441 taaaaataca acgttcatat caagctagtg caaaagttat gtcaacagct gtgcaactac
    64501 tggatgttgt aataaatgga ataatttaat agaaggggga atttataatg agagtcagta
    64561 caggtatgat gtctagcagc tatctaaact cactacaaga taatctacaa agattggata
    64621 aggttaatag acaagttaat acaacaaaag aaataaacaa gctatcagat aatccatata
    64681 aagcaataaa gatattaaac tcaaaaagtg aaataaagac tatggagact tatgttgaga
    64741 attgtaaaga tacagggaat tggttagaaa ctactgatac tgcattagat cagcttggaa
    64801 aattattatc agacataaag aaaactttgg tggcaagtgg tgatggtgca tactcagatg
    64861 atgaattaaa gactcttagt aatgaaacta atgaaaaaat gaaagaaatt gcaaatgctt
    64921 taaatgcaac acatgaaggg aagtacatat tttcaggttc aaatacgggg gtaccacctg
    64981 ttgaatgtat agataatgct gatggttcag tgagtttaaa gtttaattct agtctaaatc
    65041 ttaataaatt aaatgactct ttgagtatgg atatagctca aggaataagt gtagattata
    65101 acgttaaatt gagtagcctt ggatttgacc caacaaaaac tgacccattt gaagagttaa
    65161 ataacatatc aaaaaaatta gcaaatccag atgctgatgc tataaaagag cttacaacaa
    65221 cttgtttggg cgatatggaa caactaatag aaaatacagt aaatgttagg tctatatatg
    65281 ggacaaaagc aaatacagta gaggctatga aggaaaaaaa cgatgatagt ttgatacagt
    65341 taaaggatat tttgtcacaa aatgaagaaa tagattatgg tgaaaaactt gttcaattaa
    65401 aggcagcaga gttaacttat caagcttcac tacaaacagg aggaaagtta tttaatgtat
    65461 ctatattaga ttacatataa taaaataacg cagatatgat gaaggttaca ttaaaaaaag
    65521 gtatattagg ttttgaaaat ttaaaagaat atgagctttt agatatagaa gataatgatt
    65581 atttaaaaga atttaattct actgaagaag aaggtatagg atttgttgtt gtatctccat
    65641 ttgagattat caatgaatat gaaatagttt taaatcagga aacaatagaa aaactagaag
    65701 tgaaatctcc aaaggatgtt gtattattta atataattac tcttggtcag acatttgaag
    65761 aatcaactgt gaacatgaaa gctcctatag tgataaatat aaaaactaat tttggaatgc
    65821 aaataatttt acaggatgat aaatattcaa taaggcatcc attattgaga ggtgataatg
    65881 gatgctagta atttcaagaa aaaaagatga agcagtactt ataggagaaa gcatagaagt
    65941 aaaaattgta ggagtagatg gaaacaatgt aaaactagct atatcagcgc ctaacaatat
    66001 aagtatatta agaaaagaaa tatacgaaaa agtaaagagt gagaatataa aagctacaaa
    66061 taaaaatata aaaattttaa agtcattaaa ataataagct tggaggaagt aatatatgta
    66121 tggagaaaat ccttataatt cttataaaca aaatgctgtt tttatggcat caaaggaaca
    66181 attgttactt atgcttgttg atggagcagt aaaatatacc aaaatagcca aaacagctat
    66241 cttagataaa aatacacaaa aagcgcatag agagcttata agagttcagg atatatttac
    66301 agaactaatg gttacattag accaaaatgc aggtcaatgg gcaaaggata tgtacagagt
    66361 ttatgatttt gtaagatatg aactttcaag agctaatatt agaaaagaca ttcaaataat
    66421 agataatgtg ttgcctgtaa tagaacaaat taaagatact tggcatgaag ctgataaaaa
    66481 aagtaaagaa gaaagaagta ggtacaaata atggaattaa atttagctga attagataag
    66541 ttatcaaaag aggaattact attgatgctt gtagatggaa cagtaaaata taccaatata
    66601 tccaaagaag ctcttttaaa taacgactat ttgaaagcac ataatgaact tgtaagagtt
    66661 caaaatatat ttacagaact aatgactaca ttagaccaag atgcaggtca atgggcaaag
    66721 gatatgtata aagtttatga atttataaaa tctgaattag caatagctga tgaaaataaa
    66781 gatattaaaa taattgatga tatattgcca atagtaaaac aaattagaga tacatggcat
    66841 gaagtttaca atcaactaaa aatataattg gatggaggga taaaggtatg tcaagtataa
    66901 gtcctataag agttacaggt ctttcaggaa attttgatat ggaaggcata atcgaagcta
    66961 gtatgattag agacaaggaa aaagttgata aagcaaaaca agaacaacaa atcgttaaat
    67021 ggaagcaaga aatatataga aatgttatac aagaatcaaa agatctttat gataaatatc
    67081 taagcgtaaa ttctcctaat agtatagtaa gtgaaaaagc atactcttct acaagaataa
    67141 ccagttctga tgaaagtatt atagtagcaa aaggctcagc tggtgcagaa aaaataaatt
    67201 atcaatttgc agtttctcaa atggctgaac cagcaaaatt tactattaaa ttaaattcaa
    67261 gtgaacctat tgttcaacag tttcctccaa atgctagtgg agcgagttct ttaactatag
    67321 gagatgtaaa tataccaata tctgaacaag atactacaag tactattgta agtaagataa
    67381 actccctttg cgcagataat gatataaagg cttcttatag tgagatgaca ggtgaattga
    67441 ttatttcaag aaaacagact ggttcgtcat cagacattaa tttaagagta attggaaatg
    67501 acaatttagc tcagcaaatt gctaatgata atggtatcac atttataaat gatgctggtg
    67561 gaaacaaagt ggcaaatgta tatggaaaga atctagaagc tgatgtaact gatgaacatg
    67621 gaagagtaac tcatataagt aaagaacaaa attcatttaa tatagataat attgactata
    67681 atgtaaattc aaaaggaact gcaaagttga cttctgtcac tgatactgaa gaagctgtta
    67741 aaaatatgca agcatttgtg gatgattata ataaactgat ggacaaggtc tatggtttag
    67801 ttactactaa aaaaccaaaa gattatccgc ctcttacaga tgcccaaaaa gaagatatga
    67861 caactgaaga aatagaaaaa tgggaaaaga aagctaaaga aggtatactt agaaatgatg
    67921 atgagttaag aggttttgtt gaagatattc aatctgcatt ttttggagat ggaaaaaata
    67981 ttattgcatt aagaaaacta ggtatcaatg aaagtgaaaa ttacaataaa aaaggtcaaa
    68041 tatcatttaa tgcagatact ttttcaaagg ctcttataga tgatagtgat aaggtataca
    68101 aaacactagc aggttattct tcgaattatg atgataaggg aatgtttgaa aagctaaaag
    68161 atattgtata tgaatattct ggaagttcaa cttctaaact tcctaaaaaa gcaggtatag
    68221 aaaaaactgc ttctgctagt gaaaatgtat attcaaaaca aattgcagag caagaaagaa
    68281 atataagcag gttagttgaa aaaatgaatg ataaagagaa aagactttat gctaaatatt
    68341 cagccttaga atctttgttg aatcagtatt cttcccaaat gaattatttc tcacaagcac
    68401 agggtaatta attatggata aattaaaaga aaaattaaat ttatataaag atatatcttt
    68461 acaaattata aatttgattg aaaaggaaga atatataaag ataagtatta agttaggtga
    68521 aagacaagag attataaata gtgtaagtga aattgataga aatgatttta tacagcttta
    68581 taatagaatg gaattaattg aaattgatag caggataaga gatattttac aaggacaatt
    68641 attagaagta aaaaaagaat tacatgagta taagcttact aaacaagtga acacaatgta
    68701 ttataactta aatagagaaa aagtaaatat ttttaataaa aaagtataaa gttatgtacc
    68761 atcttgtccg attatataaa tgaagaatta ataaaaaaga ttaaaagtag aaatgacaag
    68821 gatgtcaact atactaagga gggtaaaata atgagagtta atacaaatgt aagtgctttg
    68881 atagcaaata accaaatggg aagaaacgta aatgcacaaa gtaagtctat ggagaagcta
    68941 tcttctggtg ttagaattaa gagagctgct gacgatgctg ctgggcttgc tatatctgag
    69001 aaaatgagag ctcaaataaa aggtttagac caagcaggaa gaaacgttca agatggtata
    69061 tctgttgtac aaacagcaga aggtgcatta gaagaaactg gtaacatact acaaagaatg
    69121 agaactttat cagtacaatc ttcaaatgaa actaatactg ctgaagaaag acaaaagata
    69181 gctgatgagt tactacaatt aaaagatgaa gttgaaagaa tatcaagctc aattgagttt
    69241 aatggtaaga aattacttga tggaagttct acagaaataa gattacaagt tggagcaaac
    69301 tttggaacaa atgttgcagg aacatctaac aacaacaatg aaataaaagt tgctctagta
    69361 aatacttcaa gtataatgag taaagcaggt ataacaagtt ctacaatagc aagcttaaat
    69421 gcagatggaa cttctggaac aaatgcagct aaacaaatgg tatctagctt agatgtagct
    69481 cttaaagaat taaatacatc aagagcaaaa ttaggggcac aacaaaatag actagaatca
    69541 actcaaaaca acttaaataa tactatagaa aatgttacag cagctgaatc aagaataaga
    69601 gatacagatg ttgcttcaga aatggttaac ttatctaaaa tgaatatatt agttcaagca
    69661 tcacagtcaa tgcttgctca agctaatcaa caaccacaag gagttttaca attattagga
    69721 taaaataatt ttgtatattg gatatagaag aaagggtaag gctttgcttt actcctttct
    69781 ttttttacaa atatactttt gaaaggaaaa tttatatgtt acttagtata gttatgattg
    69841 ttaaaaatga agaaaaaata cttgaaaaaa ctttaaaatc attaaccaca ttaagaaatt
    69901 ctatagaaag tgaacttata atagtcgata caggttctac agatgatact ataaaaatat
    69961 ctaagaagta cacggaaaaa gtgtattttc ataattggaa taatgatttt tctagtatga
    70021 gaaatatatc aatatcctat gctaaaggtg aatggttact tatattagat gcagatgaaa
    70081 tactaattga ttcttcaact attgtaaaat tttttaaaga tggtcttgat aaaaaattta
    70141 attctgcttc aatacaatta aaaaatcttt actcatatga taaaaaatta tatggatatt
    70201 gttcggtgct tagacttttt aaaaacgttg attttagata ccatggtaaa gtacatgaac
    70261 aaccaatata caaaaaccct atatttaata atgttgctga ttttgaacat tatggatatt
    70321 tgtttgaaga tgaagaaata agattaaata aggtgaaaag aaatgaaaaa ctattatttc
    70381 aagagttaaa agagaatgat aattctccat atacaaacta tcaattaggg aaaaatttta
    70441 ttatattagg aaaatatcag gaggctttat actatcttga gaaatcttat gaattatatt
    70501 ctaaactaga attggcacct ggttacttga taacaagcct tgtaaaaaca tatttatatt
    70561 taggaaaaca taagaagtgt gaaagattgt gcttaaagta tattaagaaa gacatgaata
    70621 atattgatat atattattat attgcacaag cacaagtaga tttaggaaag tatgaaaata
    70681 gtatagattc gtataagaga tatatttact tgttagataa ttatgaaata agtacacaag
    70741 caaatagttt attttcagac actgatactg tagggcttag agatgaggct attattacac
    70801 tgataaagat ttattataag cttgaaaaat atgagtttgt aatttcagag tataataaca
    70861 ttgaagatgt ggaaaagaaa aagaatgtgt actttagctt atttatgtca ctatataaat
    70921 tgaatagatt tgaagatata aaaaattatt ataaagaagt ttcaaattct aaaattgaaa
    70981 aaaatagttt ttatagaaat atagaagaag ttataaataa tattaaagag gatgaaaaag
    71041 agtatgtata taaaatcctt tgtgacattg aaggtaatta tggcatttta aatcaaataa
    71101 ggataaataa atgtgtatct atagataaat gtaaagaaat actcaatgaa gaaaaggaaa
    71161 taatttatgc atatattata aaaatagctt ttgaagagaa gattgattta ctagatattt
    71221 tttatgaaat ggaatatgta tggatagaga aatatctaaa atatctttta gcattagaaa
    71281 gaagttttaa tttagtacta tataagtaca taattagtcg tccaaataca tcagatatgg
    71341 aaaagataag agtatataaa gctatatcta aagtagtttt agaaagtata agcttaagtg
    71401 atgaaaaata taaggagatt ttttatcttt atataatgta ttcttataag tacataagat
    71461 acatatatag caatttaagt gattttgaat taataaaatt tgtttgtaat gatttagata
    71521 agttcactat agaatttaag catctacttg atattaaaaa taaagtaaat aatgctaatg
    71581 aaaaattaga gtatatacat agtatgagga aattactaaa tgagtatcct ttttataata
    71641 aaataataaa aatacttata ggtgaattag aagaaaatat aaaagaaagt gaagaattta
    71701 aggatttaaa agatagtttt attattagta tagaaaatat gattgaaact ggcaaaataa
    71761 atgatgcgaa attattggta gatgactatt caaaaacatt tgtggatgat ataaagattt
    71821 taaatataaa gggaatttta tacatgtttg aaaataagct tgataaagct gattttatgt
    71881 taaaaaaggc attatcatta gatgtagaaa atgaagatac tatatataat atagaatact
    71941 taaaaagttt aagataagtc aaaaatagga gaattgaaga atgaatatac caatagtggt
    72001 taatgaaaaa tctaaagaca taataaaaga ttggcaatat acatgtacaa atgaagatat
    72061 aaaatttaca agcataataa tattgacata taatcaaata gaatatacta aattatgtat
    72121 tgaaagtatt aggaaattta ccccaaagaa taaatatgaa ataataataa ttgataataa
    72181 ctcttctgat ggaacagttg agtggttaaa gggacaatct gatataaaat ctgtgtataa
    72241 taacaaaaac ttaggatttc caaaaggatg caatcaaggt attgagttag ctactggtga
    72301 aaacatattg ttgttaaata atgatgtaat agttactcca aattggctct ataatttgaa
    72361 taaagcttta tggtctcaag ataatatagg tgctgtagga cctattacga atgcatgttc
    72421 atattatcag caaattgatg ttgattataa gtcaattgag gacatgttta tatttgcaca
    72481 caaaataaat aaatctaata ttaacctatg gaaatataga ttgaagttag ttggattttg
    72541 catgttaata aaaaaaactg ttctggataa tgtaggggtt ttagatgaat tattcacacc
    72601 tggaaatttt gaagatgatg atttatcttt taggatgata gagtcaggat ataagttatt
    72661 attatgccag gatactttta tacaccattt tggaagtgtg tcttttaaag aaaatcgtaa
    72721 taaatataca gatataatgt atattaatag aagaaaattt gaaactaagt ggggatttaa
    72781 tccagaatat agtggaatga taagaacaga tttgatttct aaaatagatg atagtttgga
    72841 ggcaaatatt aatgttttag aaataggatg cggtataggg gctactcttt tagaaataaa
    72901 aaatatatat aaaaatgcat caatatatgg aatagaaatt agtgagtcag caggtaaact
    72961 tgcaaagtat agtgcagatg tacttatagc aaatatagaa aaagtaaaat tggattataa
    73021 aaaagatttt tttgattata taatattggg agatgtactt gaacatttga ttgacccatg
    73081 gaagatagtc aatgaattga aacaatatct aaagaaggat ggttatctgg tatctagtat
    73141 accaaatatt atgcatgttt ctgttttaag aaatcttgtg ttaggtaatt ttacatatga
    73201 agattcagga attttggaca agactcattt aaggtttttt acattaaatg agataacaaa
    73261 aatgtttaat gatagtaatt acataattaa tagtattaca gctactagta tgccactttc
    73321 ggaaattgat aatagcttca ttgatacaat atgtaaggaa agctgtaaag aaatgagaca
    73381 acagtacgaa tcttatcaat atatagtaaa agcctctaaa atcctagata ctaaaagata
    73441 ttctagtatt agtatgatta atttaaaata cgctttaatg agaattgata atgaattaga
    73501 tgtagatagt aatttaagtt atatatttga taattataat ataactgaac catgttttgt
    73561 tgatgatatt gaatatctaa ttgatacatt tgtgataaat aaagcaactc tattaaatag
    73621 attaggagtt gaggcattta aaagaggtct aggagatttt agtataaaca tgtttatgac
    73681 agtattagag atggatagag atgatattga ctcaatatat aatataacaa gtgtattatg
    73741 tgaattagaa gaatttgata ctgcttataa tttaattaaa aatagtagcg aaaaagtaca
    73801 gagtgaaagt gatatacaag agatactttg ttttattgag gggaagaaat atgaataatg
    73861 ataagatatg ttttataacc tgtgtaaatg atgaaaggct ttatgaagaa tgtcttgtat
    73921 atataaatag cctagacatt cctaaaaact ttagtataga aacagtagct ttaagaggag
    73981 ccgattctat tgcctctgct tataataaag ctatgagaga aacagattct aagtataagg
    74041 tatacttaca tcaagatact tttataatta ataaaaattt catacatgat attttagaga
    74101 tatttaaaaa aaatacagat gtcggaatga ttggtgtagc tggagtaaaa gatatgcctg
    74161 tgaatggaat ttggtgggat tctgataaaa aagcagggaa agtatatgat agtcatacag
    74221 gtaaaatgga tatttatgaa tttagtaact ttgaagaatg ttttaatgaa gttatggcta
    74281 tagatggatt aattatgatt actcaatatg atgtattttg gagagaggat aagtttgatg
    74341 ggtggcattt ttatgatttg tcacagagta tggagtttag acaaaataaa tacaaagtta
    74401 ttgtaccaaa acaaaataag ccttggtgta tgcatgattc tggagtagta aatataaata
    74461 atggatatga tgaatatagg ataaaatttg taaaagagta cataaatctt gatttatata
    74521 gagaaaattt ttatgaattt gataagaaga aagcaagtac catacttaag agtaataagc
    74581 atgtaatgga ttgtatgaaa gaggagctta cagaacaatt aagattaaag aattacaagg
    74641 atgttttagg tattgtttct aaaattgctc atttttctgt ttttaatcat actggtttgt
    74701 attcttgtga agaatgtgag agagcattgc tagaatgtgc aaataattta ccagaaatag
    74761 aaaatacgaa attatttaca aaaaaagatt taaaaagaag aaaagtatta catgttttaa
    74821 gtgaaggata ttcaacagga ggtcatacaa gactagttaa aaattggatt gaaattgaca
    74881 aagactctgt tcattcagtt gtaactacat ggcaatcaga tacactgcca gactggttat
    74941 taaaagcaat aaatgatagt ggtggcgaag ttatatcttt ggatttaatt tcagatgatt
    75001 atttaaaaag agctgtagct ttgaggaaaa tagcttatga ctgggctgat atagttattt
    75061 tatatgttca tatgtgggat acaatacctg ttgttgcttt tggagttgat gggaaaatac
    75121 ctattttaca tgttaatcac tatgatagta ctttttggtt agggacaagt attgcagata
    75181 ttgtcattga ttttagcgaa actagtcaat taattacatt agataaaaga ggcatagata
    75241 aatctgaaat attaccaatg ccattaaaag aaaaagaagt attttcaaaa tctgaagtta
    75301 gaaaacgata taatttagag gatgatgtga caataatatt gactattgct agtgaaagaa
    75361 aatacaatag tgtaaatgat attaattact ttaaaattgc taaaacattg attgaaaata
    75421 cagataatac aattattatt gttgtaggag ttgatacaga gaatctctat tggaaaactt
    75481 taagtaatgt tacagataat agagttatat gttttggaac aacagcaaat atagatgatt
    75541 tttataagat ttcggatata tacctagagt ctttcatggt aggaagtcat acttcaaaat
    75601 tagatgcaat tttatatgat ttaattcctg ttaagtttaa aaatgaagtt tcaccaattt
    75661 ttacaaatat gtggaatttt atagataagt ttgattataa gaatataaaa gaggttatag
    75721 acatgataag aaggtacaat actagtgaat ttgacaggga taagataaaa gcaaaacaat
    75781 cggaaataaa acattttgtt atagataaac attgtattga aataaaaaac tatatagaca
    75841 aaatatataa tgaagttaat catcataaag ttaagtatga tttatataaa aatgatgaaa
    75901 tcgaggacta tcagcttttt gtagcattat atggctatat ggtaaaaaat aaaaacatgt
    75961 cataggtggt aaaatgaaca atattcatat caataaattt gaaaaaagct gtgaaaaaaa
    76021 ctgggattat tttgtagaaa ataaatctat aaatggtaca tttctgcaaa ctagaaattt
    76081 ccttaattat catgaggagg gaaaattcac tgaccactca ttaataatat gtaaaggtga
    76141 agaaatacta gcagtcgtac ctgcatgtga aacttatgaa gatggtgaaa gaatattttt
    76201 ttctcattta gggagtactt ttggtggcat tataataaga agtggtttta atgatattag
    76261 gcatgtaaat gccattacaa ataagttaga aatatattta caggaacaaa atttcgataa
    76321 agtatattta aaaaatacat caagaatatt ttgtaaagag gatacttcat tactagaata
    76381 ttttttattt aagaataatt acatgtttta tgatgaatta agtatgtttt tagaattaag
    76441 taagtataaa gaagatatat tagccaattt taaatacagt aaaagaagag attttaaata
    76501 ttctttgaag aataatctta cctttaaaag actgtatagt gatgaagata tagtttgttt
    76561 ttttaattta ctttctaatt gtttagatag taaaggagct aaagtaatac attcagtaga
    76621 agaacttttg gagtttaaaa ataatagatt aaaagatatt gtagaatttt atgcagtata
    76681 tattgatgac attatgattt gtgggagcat ggtatttaac tttcaagaaa gagtttatca
    76741 tactcaatat ctagcatcaa attcgacata taataaatat tatccaatga tatttttata
    76801 ctataatcta ataaaagatg ctttagagag aaattttaag tatctttctt ttggaattag
    76861 cactctaaat agaggcaaat acttagatga gggtttagct acatttaaag aaggttttgg
    76921 ttcaagtgca atttgtaaca gaacatacta taaaaatata aataagtgag aatcatgttt
    76981 tatagagttt ttagagtata gtaaatatct agtactataa tattacataa aaataattag
    77041 tttataaaga aggtttgtat atgaaaagat taatgatttt aggctcaatt aagtattttg
    77101 agaatattgt tatgtcagct aaaagagagg gaatatacac aattgtttgt gataatagaa
    77161 ttgatactcc tgcgaaaaag atttgtgatg aagcaataga tgttgatgta tttgattttg
    77221 ataaaataaa gaatatagct atagataaaa agatagatgg aattttaact ggatttacag
    77281 attctttaat gaagccatat gtttatattg caaatgaact aaatttgcca tgtgtgattt
    77341 cttgcaatca acttgaatct gtaactaata aggctgttat gaaaaattat tttaaaaata
    77401 atggaatacc tacaactgat tattgtatta ttgaaagttt gaaagattta gaaaaaatac
    77461 aaggattaaa ttttccactt gtaataaaac ctgttgatgg ttatggttca agaggtgtat
    77521 attttgtatc aaacatagaa gaattaatag aaaaatttga atattctaag gaagcctcgc
    77581 aagatggaca agtaataatt gaggaatttt atgaaagtga ggaaatacaa ggactagctt
    77641 gggttcataa tggagagtca catgtatttt atattggaga tagagagctt gtaaatatac
    77701 atacaggaag agctggaaaa ccagatagat taatttatcc atcaaagtat tgttttcaat
    77761 atgaagaaga aataaaatcc atatatcaaa atataacgca ggcttttggc ataaaaaatg
    77821 gacctcttta tgtacaaatg ttagtgggag catatggaat aaaggtatct gaagtaatgc
    77881 caagattacc tggaggttgt gactatatgg ctatttctca aataacagat ttagacatag
    77941 gaagtttatt tgcaaacttt agtgtgaata atgaagtaaa ttttgatgaa ataaagaaat
    78001 ataatatgca tctatcaaaa tgtgtatatg cattaccaat atacataaga cctggcacaa
    78061 taaaaaagat aaataatata gataaaatag aaaacttaga ttatgtcact caatttctat
    78121 tactagtaaa agaaggagat acaataggat tatatgaaga tgagagacag gattgtggaa
    78181 gagtttatgg agtagcaaat aatgtatttg atgcaaatat aaagaagaaa tgtattcatg
    78241 aaatgataga aatacttgat gaaaataatg aaaatatgat agaaaatttt taaataaggg
    78301 agtatgatag atatgaagct aataactcat gaacaaatta tgtcattaaa catttctcag
    78361 aaacaaggat acgagtggat aaaagaatcc tttttaacaa aaaaagattg tcaattacca
    78421 ccaaaaataa gcatggtaga agaagggcat gtattttaca atgtaatgcc gtccctaaat
    78481 cataatcata agattggtgg aataaaaatg gttacaagat tgccagggag aaatccaagt
    78541 ttaaattcag agatatttct atataatatg gaagatgcta aattaaaggc tattttagat
    78601 ggcaattata ttacagcttt taggactgct gctgttgctg ccttggctat cgatacattt
    78661 gctaaaaaag attttaatac tattgctatt atgggtttag gtgtaactgc ttctgctata
    78721 atgaatatgc tttcatatac tcttaaagat aaaaatataa atataaagtt acttaaatat
    78781 aaagaccaag cagagtcgtt tgtaaaaaag tatgaaaaaa atgaacaatt tagttttgaa
    78841 atatgtgaca cacatgaaga acttattgta gattcagatg tagttatttc tactgtgaca
    78901 tatacagatg aatatttatc agaatttaaa tggtttaaaa aaggtgcttt attattacct
    78961 gtacatgtta tgggatttgg aaatgttgat tatctatttg ataaagtata tttggatgat
    79021 tataatcatt tgaaacatct agaaaatata gatagatata aatattcttg tgaattttat
    79081 gatgttttaa gtaaacaagc taaaggaaga gaaaatgatg atgagagaat aatatcttac
    79141 aatataggtt tgtcaataca tgatatatta tttgcaaata aaatttacga attgtgtgaa
    79201 acactaagct gtgaagaaat agaattaaaa gaaccaaaag aaaaatattg gatatagttt
    79261 atttcattca tatttacaat agtttattgt aaatatgaat atgaagtaat atattgttag
    79321 aaaattagca tgaataggat agataaattt atggataaat ataatttaat taggttcgta
    79381 tctattggaa acgaatatat aggaaattta gttgcattag aatcaaataa agcaatacct
    79441 tttgatataa agagagttta ttacatatat gatgtaccaa acaatgtaaa gagaggattt
    79501 catgcacaca aaaacttgga acaagtacta gtttgcctaa atggaagtgt gaagataaaa
    79561 tgttatgatg gtgtaaaaga gactatattt aatttacata aaaatgatgt tggattatat
    79621 ataggaacgg gagtgtggca tgagatgttt gattttgatg aaaaaactgt tttaatttct
    79681 atagcatctg aacattataa tgaagaagat tatattagag actataaaga atttttaaag
    79741 tatttaaata atatgtagtt attatacagt tagtgatgta ttttcttaat taaataaatt
    79801 taagatagcc acatagagaa tagatagatt aggatgtgaa tttattgttg gttaaattta
    79861 ataatttatt aaaacagttc caaacccata aagaagaata tgaacagaag attaaggatg
    79921 taatggaaag tggatattat atccttggaa atgaagtaaa gcattttgaa gaagaatttg
    79981 ctaactatgt aggtagcaaa tattgtgttg gtttagccag tggtttagat gcactgataa
    80041 ttgcttttag agcattagga ataagcgaag gtgatgaagt aatcgtgcaa tcaaatacat
    80101 atattgctag tgttatggga atcagtataa atggagcaac accagtattt gtagaaccaa
    80161 atgaatatta taatatagat tcatctaaga tagaagaaaa gataaatagc aaaacaaaag
    80221 ctatattagt agtgcattta tatggtcaag caagtaatat gaaagatata caagaattat
    80281 gtgataagta taacttgtat ttaattgaag attgtgctca atctcatgga gctaaatatg
    80341 atggaaaaat gacaggaaca tttggtgata taggttgttt tagtttttat ccaagtaaaa
    80401 atttgggtgc atttggagat gcaggagcta tagtaactag taatgaggaa atagctgata
    80461 aagtgaaaat tcttagaaac tatggctcag aaaagcgata ttataataaa tttgtcggat
    80521 ataattcaag attggacgaa attcaggcag gtttattaag agtaaaactt aaatatctaa
    80581 atgaaattga aattgaaaaa tcaaaaatcg tagatagata tcttaatgaa attaataatc
    80641 ccaaagtcat acttccaaag atagaaaata attgtactca tgtatggcat ttatttgttt
    80701 tacaagttga aaaacgagat acatttatgg aatacttaaa gaaaaatggt atagagtcta
    80761 taatacatta tccgatacca ccacatctat ctgaagcata tgcttatttg ggatataaaa
    80821 aggggagttt taatatagct gagaactatt cagataaagt tgttagtata cctttattta
    80881 tcggtatgga agatgaagaa gttagttata taattaaaat tataaatgaa tattaaacta
    80941 ctcataataa tatcaaaaat aaatttttta tatatcattt atttaagttt cgttaattga
    81001 catttttaaa tattgatata ttgtacaaat aaaattgaaa tatatggaaa aatatagaat
    81061 taagatgaga tttgtcttat atttttataa attaaaaata ttttcaaaga gtttattata
    81121 aaattctaga gtatagtgtt tataatattt gtaattattg gtaactaaat ataaaatata
    81181 aaatatttga ttataaacaa aaaagatatt taaaaaattt gttatttatt gtatacttaa
    81241 gttaaaacta aataggcaaa tctagagaaa tctagtgacg caaagctata gggactaagg
    81301 tttatataca tgaactatgt cagccagttg ccaaaaagag tcctaggtgt attgtatacc
    81361 taagaaaagt ctagaatgac tgcctttttg gcagtcattt tgttatgaaa aaatcacttt
    81421 atttcagtag aaaataccta taattaacat actttaccaa attataacat aaaataaaga
    81481 aaaattatct taaaaagtta ctattttaca aaaaatgact tctttattca tttaaattaa
    81541 tcgcatttcc attacattaa tacaataatg tatcatttta gtaagaaaac taatattttt
    81601 tcaatgaagt cccatgaaaa tataaaaaaa ttttaatatg tatttaaata tttcttcgaa
    81661 aagttgctct tttttgtaat atagcaactt cattttttta ttaataagca aaataaataa
    81721 aatatatttt gaggaggagt gagtgtgaat acatatgaca ttataaaaat gggattagat
    81781 gcaactaatt taagaagtca gacaatagca aataatatag caaatataaa tactgaagga
    81841 tataagagac attatgtaac ttttgaagaa acattaaatg atgctatgcc caaaatagaa
    81901 gaaaaaacag ataattctaa aggattaaga gtggatggca ataatgtaga tatagaagaa
    81961 gaaaaagtaa accaagcaat gacaacatta caatataatg ctttgataag ttttgcaaat
    82021 ggaaaaatag caatgactaa atcaataata agtgggaggt aattaaaagg tgagtgtgtt
    82081 cagtggaatg agaatatctg ctagtggatt aacagcagaa agcttgaaaa tggatatcat
    82141 atcgtccaat gtagctaata taaatgccac aagaggtcca aatggagaag catataggag
    82201 aaaagttgtt tcatttgaag aaaactatga taataaatta ggtatgctag gagtaaaagt
    82261 tacatctgtt gaagatgata aatcacctct taaaaaagta tatgacccat ctcatccaga
    82321 tgcaaagcca aatggatatg tagaatatcc aaatgtaaat atattggtag aaatgacaga
    82381 tttaataaca gcttcacgag catatgattc taacgtagat acattgaatg ctcagaagtc
    82441 tatgatatca aaagctttgg aaataggaaa gtagggataa attatgccaa ttacacaaat
    82501 taacaattta atgcaaggtg caaagttaga taatttgatt ggagaaacaa aaccattaac
    82561 tcttaataaa gaagaaaaaa agaattttga agatgtaata atagatacta tagataaagt
    82621 taattttcaa caagtgacag cagatgatat gaaagaaaaa tttataaaag gcgaagatgt
    82681 ttctatgcat gaagttatgt taataggtca agaagcacag ctatctatgc aatatttaat
    82741 agaagttaga aataagattc atgatgcata tcaagaaatg aataaggtac aaatttagcc
    82801 ttttgaacag gagtaataat caatgatagc cagttttaaa aataagatta aagagcaagt
    82861 tgctaaatct aggacaaaga taagttctct aaaaaaggga actaaaatag cattaggtat
    82921 aggggttatt gctatatgtt tagcgctagt atttacagca atatctgcga gcaaaaataa
    82981 gtatgcagtc ttattttcag aactagatgt tcaagatgct gcagatatat cagcagaatt
    83041 agagtctaaa aagatagata ctaaggtaaa gggaagtacg atttatgtac ctagaacaga
    83101 agttgataag ttaaggctgg agttagcacc aagtcttaca agtggttcta aaggatttgc
    83161 actaatggat gatagttcat caataggact tactgatgaa gagtttcaga ttaagaagca
    83221 aagaatgata caaggtgaaa tagaaaaaac aataaagagt tttccacaag tggaagacac
    83281 tagagtacat ataacatttg gagagtcatc agtatttgag aaggaggctg ttccagggag
    83341 tgcagcagtt tatttgatgc taaaaccagg gacgacttta gaaaaaaacc aaataaagtc
    83401 tgttatggca cttatatcag gaagcgttac aaatatacca gagaaaaatg ttcaggttat
    83461 tgataacaaa atgaatttat tatctgaagg tattttggag gatgaaaatg ttaatgaaaa
    83521 taatcaacca ataaatagtg gcattagtgt tgataaaaat aaggcatctg aacaagcatt
    83581 gaataaacaa ctagaaaagt ctattttaga aatactagaa ccaatatttg gtaagggaaa
    83641 ggtgaaagct acagtaaatg ctgatttaag ttttgataca gctgaaaaaa ctgagataaa
    83701 aattgaccct gataaagttg caataaaaga gacaaggtcg aaaaattcat ctaaatctgc
    83761 tacaaataat gttcaaggtg tagatgctaa catgaataac caaggaaata acaatggtac
    83821 taatacagaa gatagtttag atgagagttt tgagtatgaa acaggtaaga ttgaaactca
    83881 tactatagtt gcgcctggag aacttaatag attgacagct tctgttgcaa tagatggaaa
    83941 aatagctaaa ggtgttcaag ctgatgtaga ggatattgtt aataatgcta taggtatgga
    84001 tggagcgagg ggagatactc ttagcgttgt ttcaatggta tttgacccag aaggtaagaa
    84061 agaggctaaa caagagctgg aagagattaa aaaagaagga tttaaaaaga acataataaa
    84121 agcagttatt ggagtagtgg cagtagtagc aattggagct attggatact tgatatataa
    84181 gaaaagaagt gaacgagaag atatggatta tgaagatgaa gattatattt ctaaatctga
    84241 ttctgttgat agaaaagata atcctctatt aggagatgac ccagaactga cattagagga
    84301 agctgttaaa atgtatgcag aagaaaagcc agaccaagta acagagataa taaaaacttg
    84361 gttaaatgag taagggggat agaaagtgtc cacggaaatt acaaatgtta atgcgtttga
    84421 cttaagtgat ataaatgaga gtgataagaa agagaagata gaaggtgagg gtttaaaaaa
    84481 agctgctgta ttattaatga cattaggacc aaatgtttct agtgatataa taaaaagctt
    84541 gcctgataaa caagttcaaa aaataggttt ggaaattgca aatatttctt caatagcttc
    84601 aaaagaaaga agagaaatct taaaagagtt tgtagaaata aatagggcaa aagattatgt
    84661 tatagaaggt gggcttgaat atgcaagaga gttgttaagc aatgctttag gaaatgccaa
    84721 agcaaacaaa ttacttgaag gtatatcaat aaatacaagc acaaagcctt ttaaacatat
    84781 tataaaacta gatgtaaaga aaatattaga aatacttagg aatgaaagtg cacaaactat
    84841 agccgtttta cttgcacata tggagccaga aaatggagca gaagttttat catacttaga
    84901 ttttgattta caaaatgaag tagctatgag gataagctca atgggttcaa tatctccaga
    84961 agtaataaaa gtaattgaaa gttcattaga agataaatta cactcaatga gtggtgatga
    85021 ttttaaggat tatggaggag tgtctacctt agttcaaata ttgattaatt ctgatagaaa
    85081 aacagaaaag aatattttgg gaactattga agaacaagac ccggatttag cagctcaaat
    85141 taaatctcat atatttgtct ttgaagatat tgtcaagtta gatgatataa gtatacaaaa
    85201 agtgttaaaa gaagtgacta tgaaagattt agtatatgcc ttaaaaggtg tggatgatga
    85261 gatttcagat gctatatata gaaatcaatc ttctagggca gcagaggcac ttagagaaga
    85321 aatggaaatg gttggtgtca taaaagttac acaggttgag gctgctcaac aaaagatagc
    85381 aaatatagta agaaaacttg aaagagaagg tgccataact attgtaagga attttggtgg
    85441 tgactttgtt gaataataag attattaaaa gtaaaaaagc attatataaa ggagtgctag
    85501 atttaaccat aatagataaa gttagagtgg gagaatcaaa tgtaaataaa gaaaatgatt
    85561 gtagatttag ggatgaggta ataaaagaag ctatagaatc tgctgagata aaaaagaatg
    85621 agatattaga agaagcaaaa atgaatgcac aaaatataga aaagcaagca tatgaaaaag
    85681 ggtatagtca aggtcaaaaa aatggatatg aagatggata taaagagtca tataatgaat
    85741 atacccaaaa agctaaagat gaagcagatt ttataaaaaa tcaggcagac ttaatactat
    85801 tacaagctaa tgagaagatg actgagtaca taaaagaaaa aaataaagag attgtaagac
    85861 ttgctattaa tatggctagt tctgttctaa aaaagcattt cgaagatgaa gaatctatga
    85921 gtgatttgtt aaagcaaatt attcaagatt atgaaggaag tagttcattt atagtaagat
    85981 gtaattcttt ctataaagat aatatagaga aagagtttga attattcaaa gaagaaacat
    86041 cactaaaatc aagtgtattt gttattgcag atgatagtat agataaaggc aatgctatca
    86101 tccaaaagga aaatggaaga ataatagttg gtatcgattg tgctatggaa aaactaaaag
    86161 aagaattgct tggtgaataa agtatgtata aaactgatat atctatagat tttgacaaaa
    86221 taaataagaa aatagaaaat ttagaactca ctatatcaga aggtagagtc aaaaagataa
    86281 taggacttac tgtagaagtt gaaggaataa aagcttttgt tggagagtta tgtgttatat
    86341 acaatcaagt caataaacca gtgaattgtg aagtagtggg ttttaaagat aaagaagtca
    86401 tattaatggc gttgggagag ttaacattga tagctcctgg ttgtaaagtt atttctaagg
    86461 ggataccttt aagtgttatg tgtagcgata atttactagg caaagtactt gatggtcttg
    86521 gaaatcctat agataactca gatgctgttt taggagatag atacaacttg aacaatgaac
    86581 ctcctgatcc aatgaagaga aaaaagatac gaaatataat ggaaacaggt gtaagagcaa
    86641 tagatgcatt tactacttgt ggtgaaggac agagaatagg gatatttgca ggaagtggag
    86701 taggtaaaag tacgactcta ggtatgattg ctaggaatgc aaaagcagat gtaaatgtaa
    86761 ttgcccttat tggagaaaga ggaagagaag tattagattt tattgataaa gatttaggtg
    86821 aagagggaat gaaaaaatct gttgttgtat atgcaacatc agataaagct cctttggtta
    86881 ggctaaaagg tgctcttact gctactgcaa tagcagagta ttttagagac cagggcaaaa
    86941 aagtaatact tatgatggac tcggtaacta gatttgctat ggcacaaaga gaagttggtg
    87001 tagcaatagg agagccacca gctcaaaaag gatatacacc atctgtgttt gctattttac
    87061 caaaacttat ggagagaaca gggacatcag ataaaggttc tataactgca ttttatacag
    87121 tattagttga tggagatgac tttaatgagc caatagcaga tacaacaaga gggatacttg
    87181 atggtcatat agttttatct agagacttgg caaataagaa tcattatcct tctatagatg
    87241 tattgaacag tttaagcaga cttatgaatg agattgcttc aaaagaagac ataaaaatag
    87301 catcttttgc gagagatatg cttgctgagt atagagaagc agaagatttg ataaatatag
    87361 gtgcatatgc ttcaggtaca aacaaaaaaa tagatgaagc aatctactat catgagcata
    87421 ttattaattt tttaaaacaa ggaataaatg aaaagagctc ttttaatgaa actataagta
    87481 gtttaagagg aatctttgaa taaacaattt aaaaaaatat ttgaataggt tgtgatggaa
    87541 ttggataaaa attttaagtt tagattacag aaagttttag acttaaaaat aaaagatgaa
    87601 gaagagataa aaatggaatt tgctaagatt caacaaaaaa aaatagatat agaaactaat
    87661 ttagaaaact tagaaagtaa ttacagcaaa tattctatag gtaaaaataa tgatagtgtt
    87721 caaaatcaaa aaataactat taactatttg ctagctttaa acaactctat aatggattta
    87781 tcagaaaaac tggataagtc gacaaatgaa cttgaaaaag ctagaaaaca gcttataagc
    87841 aaacagatag agagaaagtc attagaaaaa ttaaaagaaa aaaaatatgg acagtattat
    87901 aaagaagaac agttaaaaga acaaagcact aatgatgagt ttgcaagcat gagttatttg
    87961 agaaatagac aagttctata aaatagtagg aagtgaggat attattgtat gaagatacaa
    88021 gcagattctt ataatattat taaagatatt gggaatagaa aaaactcatc taagaataaa
    88081 aatataaaaa atgataaatt tgaagataat ctatataaga caacagacca taaaaaggtt
    88141 aaagatagaa gagaaacaag taagcaaaat gttagctcag atagaccgga aaatagtgat
    88201 aatgagtgtc atttagatga agataaaagt aatcacttag aagatgaaaa tacatatgaa
    88261 aaagattgta gtaataataa tgaagctttg cagcaattac tgaatctttt aaaagagaat
    88321 tataaaaaag ataaagatat aaacactata ttgaataata taaaaaaact aagtatatca
    88381 gatgaattaa aacaagaact taagcttaat aaaaatatat taaataagat tgcaaatcaa
    88441 aattcagagc taaataaagc taatataaat aactctaatg atatagaaaa tttagacata
    88501 gttaaattaa gagaactttt ggaggataat gctaataaag atattgaagt aattactaaa
    88561 actacagaaa atgatactaa aaatatagat ttgtatagtt ttgatagcaa tagaatggat
    88621 aatcttaact caaataaaca gaaagatgat agctctgata ttttggaaaa actagcaggg
    88681 gtaaatggaa ataagagtgg gaattttgga caagtaataa ataaaaatgt tgatatgaat
    88741 aaaattaaaa atgataatgt caatttaaaa cagatagaat caaatgtaat ggatgactct
    88801 ataaaagcta ttaagcatat gaaaacaaat gatatacaag aattgacaat taaattaaga
    88861 ccaaaagaat taggagatat gaatatacaa ttgttaaaag atggtgaaag tatgagagct
    88921 gttgtgactg ttttcaataa agatgtcttt gactctataa ataaaaacat agcagattta
    88981 aagcaacatc ttgaacttac taatgtaaat ataaaagatg tatcagtgca aatgcacagc
    89041 gataatacaa atacatctga tacctttgat aaagcatttg aacaacagaa tagacaaaat
    89101 aaccaagaaa acatgagtaa gaagaataca aatgataagc ataataatgt agtcatagaa
    89161 gatgacatta aagaaaatac tattgatgat gatagagtag atttactagc ttagtaaggg
    89221 ggaagtattg catgtacgga atagcaacgg ctttggcagg aactcaggga aactataggt
    89281 caaataactt aagccaactt gaaagaaata ttaatgatat tgccaataaa caagttagaa
    89341 gcaaaggaaa tcagacagta acagacaatg ggactccaat tataggacaa ggtgatgatg
    89401 ctgataaaga cttgtttatg aagatacttg tggcacagat gggtaatcaa gacccaatga
    89461 atccgcaaga cccaacagaa tatgtgtcac aattagcaca gtttgcaact ttagaaaaga
    89521 tgtcagctat gaatgagaag ctagatgtac ttttagcctt aagtaattcc actctaataa
    89581 actctgcttt aagtatggca acatcattaa ttggtaaaga agtagaagta tctggggaag
    89641 agaaagaaga tgaaaacatt gtaggagtag taaagtccac atatataaaa gatggggaag
    89701 tacacttaga attagaagta gatggaagtg acaaaactgt aggtgttaaa tatgaaaaat
    89761 tacttaaaat aggagaggta gatgaaaatt caggagaaga aaatgattct tcagaacacg
    89821 aatctccaga agaagcataa tattaaatga gaaaagaggt aaattcatga taaaagcaat
    89881 gtattcaggt gtaagtggaa tgaaagcaaa tcaaacaaaa ttagatgtaa taggtaacaa
    89941 tgtagccaac gctggaacta cttcttttaa gaaaagttca gctagaataa cagattcatt
    90001 ttatcaaact gtactatatg caagtgctcc aacagctgca ttaggaggaa caaacttagg
    90061 acaagtaggt gtaggtagta agatatcaag tataaataaa gatatggtac aaggaaatgt
    90121 acaaccaact aatagaccat ctgatttaat gatagatgga gatggatatc ttccagtagt
    90181 aagacaagga acagttatgt atacgagaga tggttctttt aatttagata caggatttaa
    90241 agcagctgat ggtgctttga agaatattaa aggtggaaga cttgtaaatg gtgatggata
    90301 tatgttacaa ggtatgattt atgatgggga atataaagct gatactggag aatttgttaa
    90361 aaagggtgat gctaaaggaa aagagcctat tgtaatacca cttcaaagta taagtcctaa
    90421 ggatggaaca gttcaaaatg tactagagta taatatatca aaggatggaa cagtagaatt
    90481 tttattgagt gatggtcaaa gaactcaatt tgtctatgat atacctgatg gtgctaaggt
    90541 aggagataca cctgctggaa tagataaatt aaaaggtaca atacaaaaag tgcaaacata
    90601 tgcattccaa aatccagcag gactagatgc agaaggagga aacttgttta aaccatcagc
    90661 aaactcagga gacccacaaa tagcaggagc tacaggcaca ataatacaag gggcaataga
    90721 agcttccaat gtagatatag cagaagaatt tacagaaatg ataatagcaa gtaggtcttt
    90781 ccaagcaaac agtaaaataa tatcgacatc agacgagata cttcaagaaa taataaatct
    90841 taagagatag taggtacata ttatgataag agtaacggat ttaaacggaa gaaaatacat
    90901 attaaattca gaccttatag tgaggattga ttctgtacca gaaagtagga ttctccttac
    90961 aacaggagaa aaaaaccttg taaaagagtc ggtagatgag atagtagata aagtaataaa
    91021 gtataaaaga aaaataaatg taggttatat taggagcgaa aaataatgaa aaaaacagat
    91081 tcactgacat ttattggact aatagtatct acagtattag ttttagttgg tgccgcaaaa
    91141 ggaagtagtt cagggttaaa gaattttttt gatgtttcat caatattaat cacagtgctt
    91201 ggttcatttg gagctctaat gattacattt actatagatg atataaaact tataaaaaat
    91261 gcattacaat actcatttaa gacaatgagt gtatcaaagt tagatttact agaacaattt
    91321 aaaacacttt ctaaaaaagc tagaaaagaa ggtcttttat caatagagag tgatgtaatg
    91381 ggaatagaag atccatttat gaagaaaggg ttagagctgt gtattgatgg attggaaatc
    91441 aatgagataa ggtccatctt agaactagag atagattctg ttgaagatac aaataacaga
    91501 tactctaaac tatttaaaac ttggggaacc tttgcacctg cttttgggat gttgggaaca
    91561 ctaataggtt taatacaaat gttagctgac cttacatctc cagatttaat agcatctggc
    91621 atgggaaagg cattaattac aacgttttat ggttctttac ttgccaatat cgctttgaac
    91681 ccaattgcat ataatataga tgagaagact gaaaaagaaa tttatgtaaa ggaaatgatg
    91741 ttggaaggta taataagcat acaatctggg gagagcagta tagtagttga ggaacgttta
    91801 gccacatacc tttcaaacaa tgagaagtta gaaattatga aatccaataa aaatacagaa
    91861 agagcaatga gcaatggcgc gtaaaaaaaa taagaaaaag gatgagataa atccagatgc
    91921 atggctggct acttatgcag atactataac actgattctt acatttttcg tattattgta
    91981 tgcaacatca gttgtagata tggaaaaatt taaaaacata gcagatgcgt taaaaggtca
    92041 gttttctggg acaagtatat ttagtacggg ttcaagtaca agtgagaaat ctccaataga
    92101 cagtgtttta gtggataaaa atgataaatc aaatttagaa caggatttaa aagataaagt
    92161 taataattct aacttacaaa atagtgtaac agtcaaagat gatagtagag ggattttact
    92221 agagttagat gattcaattt tatttgactc tgggatttct gagttaaaag tagatagtaa
    92281 agctactctt aataaagtat atgacatgat aaagatgatg aaaaataaga tagttataga
    92341 aggccatact gataatgtac ctgtaaaatc aagtacacat gaatccaatt gggatttatc
    92401 ttctagtaga gcagtaagtg ttgtaagata ctttgtagaa gaaaagggca tggaccctag
    92461 actattttcg gcaacaggat gtggagagta tcaacctttg gtttcaaatg aaacagcaaa
    92521 gggaagggct gagaatagaa gagttaatat acttataact attgactagg aggaagaggt
    92581 ttgttcaaaa ataaaaaaat tattataatt attttgatac tgattatttt ctcagcggga
    92641 gcaggttttt ttatattagg agaaggtgga aataaacaag ttgacaagat tgcaggtttg
    92701 tttattccaa caggaaaagg taaagatgaa gtaaagaccg aaaatcttga cttagaagag
    92761 atggtattaa agttagctga tgaaggtgta agatatttaa aagtatcaat aactgtatct
    92821 tatgattctt catataaaga tatagaaaaa aatacgcctt taattaggga taatattata
    92881 aattgtttta tggcaaaaag ggcagatgat tttactgctg ataatttaaa gaacataaaa
    92941 gaagaaatta aaaatacatt aaatatacaa ctaggtgaaa atgtaattca aaatatatat
    93001 ttcacaaata tagtagtgca ataaaaggat ggtaagacat gatactttat ttactaaaaa
    93061 tattaatatg tatcccaata attttaatat tgatagtctt atctttaaaa attagccaag
    93121 gaactttatt aaaaaaatat aatgataaat atgtaaaaat actagatatt gtaaatataa
    93181 gtaaaaacaa tagcataata attttaaaaa taggtgaaga aggatgcatt atggcatctt
    93241 cttcttctag catgagtaaa ataaaagatt tgaccaaaga agaagtatta aaaatagaaa
    93301 acgacattaa agaaagaaat tttaaaattc aagattttaa aatggaaaga attaaaaaga
    93361 aatatgattt cagtagatat attggaaaat ttacaaaaaa ggaagataag taaatggaac
    93421 tattatcaaa attacctttg gctgatgtac catatacaag ttcaatgcaa ttgtttttat
    93481 ttctaactgc attaatgttt ttaccatttg taatgttcat gatgacaagc tttgtcagaa
    93541 tagttataag tttatcattt ttgaaatcag cattaggagc acagcaagca attccaagtc
    93601 aaattttggt aggacttgca atagtattga caatatttat aatgagacca gtattaaatg
    93661 agataaacga aaaggcatta caaccatata tgaaagaaga agttactatg gaagaagcta
    93721 tgaaggaagc agaaggacct attaaagagt ttttattaac acaaaccaga cagacagatt
    93781 tagatttatt tgtagaacag gctggtttaa aagaaaagaa attaactcgt gaaaatatac
    93841 cactatctgt tgtagttcca gcatttgcta taagcgaact aaaaactgct tttcaaatag
    93901 gttttttaat atatatacca tttttgataa tagaccttgt tgtggcaagt gttttgatgt
    93961 ctatgggtat gtttatgttg cctccagtca tgatatcatt accatttaag ttattattat
    94021 ttgtaatggt ggatggatgg aacttgatag taaaaacatt gatattggga tttggatagg
    94081 agaaaaatag tatgagtgaa tttatgataa tgagtgttct taaaaatggc atgtttacag
    94141 gagctatgat tgcagctcca gtactcattg tttctcttgt agttgggctt ttaataagtg
    94201 tgtttcaagc tacaactcaa atccaggagc aaacattgac ttttgtacca aaactaatag
    94261 ctattctttt agtagtagct atcggtggaa gctggatgtt acatactctt attagtttta
    94321 cttctgagat attcaatatg atatctctta taactaaata agtaggatac atatgatttt
    94381 tgtatttatt agaatagtag cattctttgg aacactaaga atactatttc cagctggtac
    94441 acctgcttta tttaagtcac tctttgcaat cattatatca atattgattt caagtactat
    94501 gcaaattgaa tatgcaacaa atatagacaa cattgtattg tttaccttat atagtgtaaa
    94561 tgagaccata actgggatta tgcttggata tataaccaac ctatgttttt atagtatcag
    94621 aatggcgggt tctatgatgg accaacagat gggattatca atgattaata tgtttgaccc
    94681 aaattcaatg actcagacaa ctttaattga taatcttatg aactggacag cacttatgat
    94741 attttttaac atggatggtc atcatgtttt aattagaggt ataaggtata gttttgagtt
    94801 aattccaata ggaaaaccat ttgtagataa taacatagat tatataataa acatatttgt
    94861 gcagtgcttt ttaactggat tcaaaatagc aatacctatt gtactttgtc tgcttatggc
    94921 agattttatt ttagggctta tatcaagaag tgtacctcaa ttaaatgtaa tgatagttgg
    94981 tatgccatta aaaatattgg ttggtattgc attatttatc atatcaatac ctttaattgt
    95041 caatcaaata agtcatttat taagtcaaat accaaagatg tatgagggaa cttttgcact
    95101 agctcctatg ttttttatgg gttcagcaga taaaacagaa gaagctactc caaagaaaaa
    95161 gggagaacaa agaaagaaag gtaatatagc aaagagtaga gaattacccg ttgcaatgac
    95221 tctacttgca ttcaccttat tagtacctac attattttca tatgtagtag atacacttaa
    95281 atcttcgctt aattattttt tgagtctaga tttttatatg aacataaatt attcaaacct
    95341 agagaaatta gttatagcag gattaatgga tttttttaag atatttttac caatagctat
    95401 tcccttttta gttttaggca taatagcaaa cctatttcag gtgggaatac tgtttactgg
    95461 tgaaacttta aaaccaaatt tatcaaaatt aaatccaatt agtggattca aaaacatgtt
    95521 ttctatgaga agtttgtcaa ccttaataaa ggatacagcg attatatcta ttttggctta
    95581 cataggatat acattcttcc aagataatta tttagatata ttaaagttag gtaacatata
    95641 cctaccaact ttaatgtaca cagttaaaga cttagtatat tcaatattga gtaagatttg
    95701 tgtagctatg atagctatag cagttgcaga ttatgtttat caaagataca gtcataaaaa
    95761 gcaactgaga atgacaaaac aagaagttaa agatgaatat aaaaactcag aaggagaccc
    95821 agaagtaaaa gctaagataa aacaaaaaca aagacaaatt tcgtctcaaa ggacaatgca
    95881 agcagtacca agtgctactg taatagtaac caatccaacc cacttatcta tagcagtgag
    95941 atatgaaaaa gggaaagacc aagctccagt agtggtggca aaaggtgcag attatttagc
    96001 ttttaaaata agggaaattg caaagggaaa tgacatacct ataatagaaa ataaacctat
    96061 tgctaggttg ttatataagc aagtagaaat agaccaagaa attccagagg acatgtatca
    96121 agcttttgca gagatattag tagcagtata taaaatcaaa aatagataca aagtccccaa
    96181 aaggtaggat aagtaatgaa taagtttaat ttaaaggaaa atatggacgt aatagtggca
    96241 tttggtgttg tagggattgt tcttatgatg atagtgccgt tacctacatt tatattagat
    96301 attttactgg caattaacat aagtttatct gtgctaattt tactaatgac actatttaca
    96361 acaaatgtac ttgatttatc tatatttcca acagtgttac ttgtgacaac actatttaga
    96421 ttgggattaa atctttcatc aactagattg attttaacac agggatatgc aggtgaagtt
    96481 atagaggcat ttggaagttt tgttgtaggt ggaaattacg tagtaggaat aattatattc
    96541 ttaattatat taattgttca atttgttgtt ataaccaatg gttctggaag agtatctgaa
    96601 gtaacagcta gatttacgtt agatgccatg ccaggtaagc agatgagtat agatgcagat
    96661 ttaaatgcag gagttataga tgacaaaact gctagaaaaa gaagaaaaga gcttcagcaa
    96721 gaagcagatt tttatggctc tatggatgga gcaaataagt ttgtaaaggg agatgccata
    96781 gcaggtctta tagtaacagc aataaacata ataggtggaa ttgtaatagg agctgtaatg
    96841 ctgaatatga ctcttatggg tgcagcacag acatatgtaa gacttactat aggggatggt
    96901 cttgtaagcc agatacctgc tctattaata tctacatcag caggtataat cgtaacaaga
    96961 gcttctactg atgaaaactt tggtggatta gtaggtaaac agttaacagc aataccaaaa
    97021 gcagtaatga tgactggctg tgttctaatc gtacttggta tgatgcctgg attacctaaa
    97081 ccagcatttt tttcactagg tataggtgct atagcagcag gttacttttt aggaagagaa
    97141 aagaaagata ctttagaaga cttggagttt gaaaaacctg atatggtaaa tccagataaa
    97201 aatgaagtag aagatgtaac aaacttaata aatgtagaat caatagaagt agaaattgga
    97261 tatggtttaa tatctttagc tgatgaatca gcaggaggag atttacttca gagaatagca
    97321 tcaataagaa gacaatgtgc aatagacatg ggtatagtcg tacaacctat aagaataaga
    97381 gataatttac agttaaatcc aaaccaatac aatataaaaa taaaagggaa tgtagttggt
    97441 gggtatgaga taatgcctaa tatggtttta tgtatggatc ctatgaatca aggatttagt
    97501 atatttggaa taaagacaat tgagcctact tttggaatag aggcactttg gatagaaagt
    97561 gacaaggtag aagatgcaga gctaaagggt tatacagttg tagattcatc cacaatactt
    97621 ataactcact tactagatat aataaaatca aatgcacacg aattactggg aagacaagaa
    97681 gttaagacta ttatagatgc agcaagagaa aattatagtg ctgtagtaga tgaattgata
    97741 ccagatttaa tgacacttgg tgaaattcaa aaagtactta aaaatctgtt aagagaaaaa
    97801 gtaaatataa aggatagagt tacaatactt gagacacttg cagaccagtc tagaaataca
    97861 aaagatatag agcttcttac tgaatatgta agaatagctt tggctagaag tatttgtaat
    97921 aatttagttg atgaagataa ggcaattgta gtggctacat tgtctccaga aactgaagaa
    97981 ttggtatcta ataatttaca aaggtctgta aatggaactt atccagcagt tgacccagaa
    98041 aatacaaata aaatatttga gagtatacaa gaagtaatga acaatgtata tttcaatcat
    98101 aatataccag taatagtagt atcaccaaag ataagagctc catttagaaa attagttgaa
    98161 attgtatatc ctaatttgac tgtattatca ttaaatgaaa tacctaacga tattaaaatc
    98221 aaagcagaag gtgtggtgag catatatgat gacgaaaaag tatacagcta acacaattca
    98281 agatgctatg aatctagcca aaatggaatt gggtgacaat attacattaa tagataaaaa
    98341 agaggtaaga aaatctggaa taagaggcat tttttcaaag aaagatattg aattgactat
    98401 tggatgggaa aagaaagata atttagaaca aaaagattta aaacgagaga tagaacaact
    98461 aaagtcaatt ataaataata tggggtttga taataaaaat gataatgata tagataaaat
    98521 atgcaagaat ttactaaact tagatttaaa tcaagaaatt gtagaattta taaaagctga
    98581 tttacaagaa atgaagttca atggaattga tacaagtaaa aatttagtag aaagtctaaa
    98641 gaaaaagatt aaaatagaaa atcaagcaat aaatggtaaa attgctttag ttggaccacc
    98701 aggagtaggt aaaacgacta caatagcaaa gttggctgcc aaattagttt ttgaggaaaa
    98761 taaaaaagta ggagtaataa ctatagatac atacaggatt ggtgctgtag aacagttaaa
    98821 gatatatact gatataatga atattccttt taaaggggta ataagccctg atgagatgga
    98881 attggcactt gatgcgatga aagattgtga tgttgtatta atagatacaa caggtagagg
    98941 ctataaaaac tctatgcaaa tattagaaat caaaaatctt atagataaag ctgaaactga
    99001 taatatacat ttagttttga actgtacaac aagagaaagt gatacaaaag caattataga
    99061 ttcatataga aatgtgaatt ttaaaagctt aataattaca aagttagatg aaacaataac
    99121 atatggctca atatttaaca taatgaatta tgcacaaaaa ccaatatcat atattacaac
    99181 aggacaaaat gttcctgatg atattataaa acctaatgaa gataagatta taagattact
    99241 attaggagtt gaaagtatat gatagaccaa gctcaaattt tgagaaaaat ggcaatggaa
    99301 aaaagaggtt ctgatgagat tatagtagag aatgagaata tgcctaagat aattacaata
    99361 gcttctggta aaggcggcgt tggaaagagt aatctcgcta caaatttgtc tatatgctta
    99421 accaaacttg ataaaaaagt tcttatatta gatgcagata taggaatgtc taatatagat
    99481 ataataatgg gggtaaatgt aaaagggact ataattgatg taattaatgg cgaaaaaaat
    99541 atagaagata taatttcaca gactaagtat ggtgtaaatg taatatctgg aggttctgcc
    99601 cttaatcata tagaagactt tacagaagct caaagaaata agtttataca tagcatagaa
    99661 cagattcaca atgtggattt tataataatt gatacaggag ctggtatgag caaaagccta
    99721 ttgtcattta tatactgtag tactgaattt ttcttaataa caactccaga actgacatca
    99781 ttgactgacg catatagttt attaaaagct ataagtaatt ttggcataaa gaaaagtgca
    99841 aatataataa tcaatagggt aattgattta gaagaagcga aatctacata caaaagaata
    99901 aaaatggttg tggacaaatt tctaaacctt aatctggaat tgtatggata tataatagat
    99961 gacaaaaaag taagtttatg tgtaaaaaaa cagataccat tcattttaga atatcctaca
   100021 agtattgcat caaaatgtat tatagccata gcaaagagaa tggtaagtca gtcaagagaa
   100081 gatattgttg ataataaaag ttttctaaga aagatgttta gtatatttaa aagctaagga
   100141 ggcgtagtta atgaatagag aagaattaat aaaagagaat atgcctcttg taaagagtat
   100201 agcaagaaaa ttttttatac cagggaaagg ttttgaatat caagacttag taaatagtgg
   100261 agtcattgga ttgattgatg caataaataa atttgatgct gaaaaagggg caaagttttc
   100321 tagttactct tatataaaaa taaaatctgc tatattagat gaaattagaa atcaaagtcc
   100381 aatttctaaa cataatctaa ctaaagtaaa taagtataat agagtggtag aaaagttgca
   100441 atctaaatta ttaagagagc caacttcata tgaaattgca aaagaattaa aggtttcaga
   100501 aaaagaactt catgatatcg aaagtaatat agatatgtta aatattgtat cattaaacta
   100561 tgtaatcttt gaagatacaa atgaaactgt acaagatgta ataagtgata gagaagagga
   100621 agctccagaa aatataatag aagaagaaga aaagttagag attctatcca aagcaattag
   100681 taatttaaat gaaagagaaa aattaatact ttcactgtac tattatgaag atttaaatct
   100741 aaaggagata gggaaagtgc taggtgtttc agaatcaaga gtttcacaac tccacagaaa
   100801 atcaataaga aacttaagaa acaagataaa agaacttaaa tattctatat agatggtgat
   100861 tgataagatg atacaaatta tattaatagt agtgtctatt ttaataatag taactatagt
   100921 tataaattta cataaggaat caaaaaataa agataacaga tactttgata aaatatttga
   100981 gcaagaatat gaagcaaatg atagattaat aattgcagaa aaaagaagat tctttttaga
   101041 caaagtactt gctgaaataa gaagtaataa tcatataaaa gtttatgaaa attataacct
   101101 aataaaggct aatgaagaaa gaaaggaaaa catatttata gaatcaaaaa aggaagtaag
   101161 acaagaaaat gagttatctc ttaaagtgaa acatcttata agttcaggtt atgatgatgt
   101221 ggaaatatgt aagatgctag atattggaaa gggggaactg tcattaataa ggagttgtta
   101281 caaaatataa ggatattatt atgtgataaa aaagtattct taggaatagg tattggtata
   101341 ttaatatcaa ctttatgtat aatgcttttt agtaataatg atatgagtaa ggcagagata
   101401 gaaaaaaagg ccagagcgtt aggcatgaaa tatacaagtg agatgaaagt cttagaatag
   101461 atgatactgt ttagacattt acaaaaaagg aggtcataat gtgataagag gattatatac
   101521 agcagtttct gctatgataa ctaatcaaca aaagcaaaat gttgttgtca ataacctagc
   101581 taatatggat acaaatggat ataaaagtaa acaacttttg actaaaagtt ttgatgaatt
   101641 aaagttggaa ggttacaata actacgcaaa tggaactaaa caaaagcaga ttataggcga
   101701 tataagtcca ggtatttcta tggatgaaac tatcacaaat tttaatcaag gacctataaa
   101761 aactactgat aataaaatgg atgtagctat tcaaggtaag ggattttttc tagttagtga
   101821 tgcagctgga aatcaatttt acactagaaa tgggaacttc agaacagaca atcaaggtga
   101881 ccttataaca agtgaagggt attatgtact tgggacgaat actgctactg gtgctgtagg
   101941 tcatataaat gtaggaaatc aaaaatttga agtggcaaaa gataatacaa tatcactaga
   102001 cggaaatgct atgtataaat ttaatattgt agattttaat aactacaata acttaaaaaa
   102061 agaaggagat aacttatatt ctggaggagg agctatgaaa gtaaataatg cacttacaat
   102121 acaaagtgcg atagagtcct ctaatgtaga tatggtatct gaaattaata atatgatgac
   102181 aatatcaaga gaatatgagg caaatcaaaa gataattcag gctatggatt ctaaattagc
   102241 aaaaatagca agtgagattg gttcagtaag ataggtaata ggaggtgaat ttttatgtac
   102301 acaatgtttt atactagtaa aacagcttta tcagcaaatc aaagtaaact agatataata
   102361 tctaacaata tagcaaattc tgattcaaca ggatataaaa aaataaatat ggaatattct
   102421 gatttagtgg atgaagtact aaatagacca tcatacccta caaatggtaa agatatatcg
   102481 actggaacag gagctaaggc aacagctccc atgagaaatt atgaacaagg tgcattggct
   102541 cctacagata gtaagagtaa tttggcgata gatggagaag gattctttag agtgataaga
   102601 aaagatggta catatgcata tacaagaaat ggtggattta atgtagatgc tttaggtaag
   102661 attgtagatg ataatggaaa tatattagat gtccaatttg atgcaggata taactataat
   102721 aatacaaata tagatagtac aaacctgaca gtttcaagaa atggagagct attcgtagat
   102781 aataaaaaaa taggaaaaat caacttatat caacctatag gaactcaaaa ttttatatct
   102841 gaaggagata gtttattcgt cgcaactgat gcagcacaga ttaaacaagt tgagaaagtc
   102901 gatatagttc aaggctatag agagaggtca aatgtttctt tacaagaaga atttgttgac
   102961 ttaatagcca ctcaaagagc atttcaaatg aactcaaagg gaataaagac agcagatgaa
   103021 atgtggcaaa tagcgaataa tttaagagct aaataattat gtaaggtgag gaatattcat
   103081 gaatgatatg aaagagtacg attttggcaa acctcagagt tattcaagag aaaatttaac
   103141 ttgtttaaat tttttattag ctgaattttg taaaaaatat aaaaactata taatttatga
   103201 acttaagtgt aattctagta tggttgttga tagtatagac caagtaaatt atcagacttt
   103261 tttagataga gtaacttctt cgtgcgtagt agtacaaaat gcaatggaac ctgttatgaa
   103321 aaacttttct tttagaatag atagaagtgt agctgatatg tggattgata taacttctgg
   103381 aggaactgga gttgtaaagg ataatgatag agagcttaca gaacttgata aaaaagtgct
   103441 tttacacctt atggatgatt tagtaagaaa tatgcactta tttgagggtt ttgaaaatat
   103501 acaagtattg gatacacata cacatattaa tttaccacaa ttatgttctc caactgcacc
   103561 agtatgcgtt gtagatttaa aagtgctaaa tgataatgag tatattggaa gtgtttctct
   103621 ttgttttcca tacaatggtt tagaagctct gttagaaagt atatctgtta tagaattttt
   103681 tgataatgat ataggagatg attcagagga atttaccaaa aaaatatata atagtgtatc
   103741 taatatagaa ttatcggtta ttgcagagat aggaaaagta tcaattaatg ttgaagactt
   103801 acttaaacta gaagttggag atgtaatagt aacaaataaa aaaataaatg attatataga
   103861 tatatttgta gaaaattcaa aatcatatac agcaacacca ggatttatat caagtaaaaa
   103921 aggtgtaaaa ataaatgatg ctgttggaaa agaggtgtag ttatggacaa tagtaatctt
   103981 atttcagatg aagaagcaaa gatgctatta aatgataaag ttgaaagtga agttgatgga
   104041 gatgatgagt tagccttaga tgtgtctgaa gttaatcgaa ataatataca aaggatactt
   104101 gaccttaagt tagaattatc tgtggttata ggaagaacta agatgactct aagagatatt
   104161 ttaaacctac ataaaggttc tcttattgaa ctggatactt tagcagacca agatgtggaa
   104221 atactaatag ataataaagt gttggcttat ggaaaagtag tggtggtaga tttaaatttt
   104281 ggtgtaaaga taacaagtat agtaagtgaa gaagatatgg taaaatctct tgtttagaga
   104341 taacatcaat taatacttaa ggcatgaggt ggtacttatt ttaaacttaa atgatataaa
   104401 aaaagatata ttaaaactca ataaaaaaga ctcatgttct ctatataatg aatctattaa
   104461 taatataaat aataattgta tatcgagtgg attgaaattt ttaaaacaag caagagattt
   104521 aaacccagat gatgttgata tattaaatct cacaggtctt gtaaatttac taaaatgtaa
   104581 ttttgatgag gctgtagaaa gtttctataa gagttcactt tgtgaaaaaa ctgctctgtg
   104641 tgaaaagtat ataaacatgc ttacatctga ggagttttta attttctttg aaaggtacaa
   104701 tcaactcata aggttaataa acagaggaat gtataaagaa gctatacaag ggtttaaact
   104761 tataactgag caatactgtg atttaataga accttatgaa ttgcttgcct tactgtatga
   104821 caaggaagaa gaatatataa aatttgatga atgtttagaa gtattgaaga ttattgataa
   104881 ggaaaattat ttattaaatc aagaaaataa cacattagat atgtataata gtgctaatat
   104941 acaaactaaa agtaatataa aagtagatga ttataagaat gagttcttac ataaaagtag
   105001 tagtaaccat aaaattaatg attataatga tgtaactaaa gaaaagagtt caagcaaaaa
   105061 ccagaaaagt tcagattcta ataagaaaaa aagtattaaa aaaaataaat acctatatat
   105121 agccataata ttagga
//
LOCUS       DAKBNN010000002        76586 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000002 DAKBNN010000000
VERSION     DAKBNN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.002"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            439..2109
                     /locus_tag="OUB02_000106"
     CDS             439..2109
                     /locus_tag="OUB02_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HCT8883924.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHVDIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2231..2965)
                     /gene="pflA"
                     /locus_tag="OUB02_000107"
     CDS             complement(2231..2965)
                     /gene="pflA"
                     /locus_tag="OUB02_000107"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCT8883925.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3125..5356)
                     /gene="pflB"
                     /locus_tag="OUB02_000108"
     CDS             complement(3125..5356)
                     /gene="pflB"
                     /locus_tag="OUB02_000108"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCT8883926.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDFFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5649..6710
                     /locus_tag="OUB02_000109"
     CDS             5649..6710
                     /locus_tag="OUB02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCT8883927.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTSKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            6801..8414
                     /locus_tag="OUB02_000110"
     CDS             6801..8414
                     /locus_tag="OUB02_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT8883928.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8689..10674
                     /locus_tag="OUB02_000111"
     CDS             8689..10674
                     /locus_tag="OUB02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT8883929.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKDLLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            10679..11113
                     /locus_tag="OUB02_000112"
     CDS             10679..11113
                     /locus_tag="OUB02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCT8883930.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11142..11702
                     /gene="srlA"
                     /locus_tag="OUB02_000113"
     CDS             11142..11702
                     /gene="srlA"
                     /locus_tag="OUB02_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCT8883931.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            11766..12134
                     /locus_tag="OUB02_000114"
     CDS             11766..12134
                     /locus_tag="OUB02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HCT8883932.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12159..12725
                     /locus_tag="OUB02_000115"
     CDS             12159..12725
                     /locus_tag="OUB02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCT8883933.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            12766..13131
                     /locus_tag="OUB02_000116"
     CDS             12766..13131
                     /locus_tag="OUB02_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCT8883934.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13232..14005
                     /gene="srlD"
                     /locus_tag="OUB02_000117"
     CDS             13232..14005
                     /gene="srlD"
                     /locus_tag="OUB02_000117"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCT8883935.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14354..16498
                     /locus_tag="OUB02_000118"
     CDS             14354..16498
                     /locus_tag="OUB02_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCT8883936.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16586..16921
                     /gene="spoIIAA"
                     /locus_tag="OUB02_000119"
     CDS             16586..16921
                     /gene="spoIIAA"
                     /locus_tag="OUB02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HCT8883937.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            16935..17363
                     /locus_tag="OUB02_000120"
     CDS             16935..17363
                     /locus_tag="OUB02_000120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HCT8883938.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17386..18150
                     /gene="sigF"
                     /locus_tag="OUB02_000121"
     CDS             17386..18150
                     /gene="sigF"
                     /locus_tag="OUB02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HCT8883939.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18430..18867
                     /gene="spoVAC"
                     /locus_tag="OUB02_000122"
     CDS             18430..18867
                     /gene="spoVAC"
                     /locus_tag="OUB02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HCT8883940.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19034..20047
                     /gene="spoVAD"
                     /locus_tag="OUB02_000123"
     CDS             19034..20047
                     /gene="spoVAD"
                     /locus_tag="OUB02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HCT8883941.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20061..20423
                     /gene="spoVAE"
                     /locus_tag="OUB02_000124"
     CDS             20061..20423
                     /gene="spoVAE"
                     /locus_tag="OUB02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HCT8883942.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20606..21622
                     /locus_tag="OUB02_000125"
     CDS             20606..21622
                     /locus_tag="OUB02_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT8883943.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            21996..22838
                     /locus_tag="OUB02_000126"
     CDS             21996..22838
                     /locus_tag="OUB02_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HCT8883944.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAVTASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            22853..23317
                     /locus_tag="OUB02_000127"
     CDS             22853..23317
                     /locus_tag="OUB02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883945.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23304..24476
                     /locus_tag="OUB02_000128"
     CDS             23304..24476
                     /locus_tag="OUB02_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8883946.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            24520..25455
                     /locus_tag="OUB02_000129"
     CDS             24520..25455
                     /locus_tag="OUB02_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HCT8883947.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(25730..28423)
                     /locus_tag="OUB02_000130"
     CDS             complement(25730..28423)
                     /locus_tag="OUB02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HCT8883948.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     GSTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            28637..29335
                     /gene="yunB"
                     /locus_tag="OUB02_000131"
     CDS             28637..29335
                     /gene="yunB"
                     /locus_tag="OUB02_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HCT8883949.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(29547..30554)
                     /locus_tag="OUB02_000132"
     CDS             complement(29547..30554)
                     /locus_tag="OUB02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HCT8883950.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            30782..31666
                     /locus_tag="OUB02_000133"
     CDS             30782..31666
                     /locus_tag="OUB02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8883951.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            31868..33418
                     /locus_tag="OUB02_000134"
     CDS             31868..33418
                     /locus_tag="OUB02_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT8883952.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETEDLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            33447..34094
                     /locus_tag="OUB02_000135"
     CDS             33447..34094
                     /locus_tag="OUB02_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCT8883953.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(34236..34496)
                     /locus_tag="OUB02_000136"
     CDS             complement(34236..34496)
                     /locus_tag="OUB02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HCT8883954.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            34713..35996
                     /gene="hflX"
                     /locus_tag="OUB02_000137"
     CDS             34713..35996
                     /gene="hflX"
                     /locus_tag="OUB02_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCT8883955.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHIFNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            36004..36681
                     /locus_tag="OUB02_000138"
     CDS             36004..36681
                     /locus_tag="OUB02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCT8883956.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            36767..37210
                     /locus_tag="OUB02_000139"
     CDS             36767..37210
                     /locus_tag="OUB02_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT8883957.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            37515..38159
                     /locus_tag="OUB02_000140"
     CDS             37515..38159
                     /locus_tag="OUB02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCT8883958.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(38245..38622)
                     /locus_tag="OUB02_000141"
     CDS             complement(38245..38622)
                     /locus_tag="OUB02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCT8883959.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(38622..39008)
                     /locus_tag="OUB02_000142"
     CDS             complement(38622..39008)
                     /locus_tag="OUB02_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCT8883960.1"
                     /translation="MKKTDDLKQKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            39187..39942
                     /gene="nadE"
                     /locus_tag="OUB02_000143"
     CDS             39187..39942
                     /gene="nadE"
                     /locus_tag="OUB02_000143"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HCT8883961.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            40025..40744
                     /locus_tag="OUB02_000144"
     CDS             40025..40744
                     /locus_tag="OUB02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT8883962.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(40911..41915)
                     /locus_tag="OUB02_000145"
     CDS             complement(40911..41915)
                     /locus_tag="OUB02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCT8883963.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            42182..43078
                     /locus_tag="OUB02_000146"
     CDS             42182..43078
                     /locus_tag="OUB02_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HCT8883964.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHINIKSDSCNC"
     gene            43216..44478
                     /locus_tag="OUB02_000147"
     CDS             43216..44478
                     /locus_tag="OUB02_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883965.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            44554..45747
                     /locus_tag="OUB02_000148"
     CDS             44554..45747
                     /locus_tag="OUB02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HCT8883966.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            46376..47170
                     /locus_tag="OUB02_000149"
     CDS             46376..47170
                     /locus_tag="OUB02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCT8883967.1"
                     /translation="MESVMENLNNLKVELKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            47265..48605
                     /locus_tag="OUB02_000150"
     CDS             47265..48605
                     /locus_tag="OUB02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCT8883968.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            48742..49974
                     /locus_tag="OUB02_000151"
     CDS             48742..49974
                     /locus_tag="OUB02_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HCT8883969.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            50014..51156
                     /locus_tag="OUB02_000152"
     CDS             50014..51156
                     /locus_tag="OUB02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCT8883970.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            51167..51961
                     /locus_tag="OUB02_000153"
     CDS             51167..51961
                     /locus_tag="OUB02_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCT8883971.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            51972..52994
                     /locus_tag="OUB02_000154"
     CDS             51972..52994
                     /locus_tag="OUB02_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCT8883972.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            53093..54469
                     /locus_tag="OUB02_000155"
     CDS             53093..54469
                     /locus_tag="OUB02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HCT8883973.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(54801..55145)
                     /locus_tag="OUB02_000156"
     CDS             complement(54801..55145)
                     /locus_tag="OUB02_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8883974.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            55633..56643
                     /locus_tag="OUB02_000157"
     CDS             55633..56643
                     /locus_tag="OUB02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HCT8883975.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            56779..57267
                     /locus_tag="OUB02_000158"
     CDS             56779..57267
                     /locus_tag="OUB02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8883976.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            57443..59371
                     /locus_tag="OUB02_000159"
     CDS             57443..59371
                     /locus_tag="OUB02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8883977.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALEEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            59548..59958
                     /locus_tag="OUB02_000160"
     CDS             59548..59958
                     /locus_tag="OUB02_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCT8883978.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            59980..60282
                     /locus_tag="OUB02_000161"
     CDS             59980..60282
                     /locus_tag="OUB02_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HCT8883979.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(60499..61191)
                     /locus_tag="OUB02_000162"
     CDS             complement(60499..61191)
                     /locus_tag="OUB02_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCT8883980.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(61217..62404)
                     /locus_tag="OUB02_000163"
     CDS             complement(61217..62404)
                     /locus_tag="OUB02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8883981.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            62740..64617
                     /locus_tag="OUB02_000164"
     CDS             62740..64617
                     /locus_tag="OUB02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8883982.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQENQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEIIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            64927..66333
                     /locus_tag="OUB02_000165"
     CDS             64927..66333
                     /locus_tag="OUB02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT8883983.1"
                     /translation="MNTGFPKDFLLGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHSFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            66361..67209
                     /locus_tag="OUB02_000166"
     CDS             66361..67209
                     /locus_tag="OUB02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8883984.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            67870..68562
                     /locus_tag="OUB02_000167"
     CDS             67870..68562
                     /locus_tag="OUB02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8883985.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            68559..69494
                     /locus_tag="OUB02_000168"
     CDS             68559..69494
                     /locus_tag="OUB02_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8883986.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            69642..70565
                     /locus_tag="OUB02_000169"
     CDS             69642..70565
                     /locus_tag="OUB02_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8883987.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            70549..71319
                     /locus_tag="OUB02_000170"
     CDS             70549..71319
                     /locus_tag="OUB02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8883988.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            71329..72111
                     /locus_tag="OUB02_000171"
     CDS             71329..72111
                     /locus_tag="OUB02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8883989.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            72488..73024
                     /locus_tag="OUB02_000172"
     CDS             72488..73024
                     /locus_tag="OUB02_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HCT8883990.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            73056..73445
                     /locus_tag="OUB02_000173"
     CDS             73056..73445
                     /locus_tag="OUB02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCT8883991.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            73482..73868
                     /locus_tag="OUB02_000174"
     CDS             73482..73868
                     /locus_tag="OUB02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HCT8883992.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            73995..75437
                     /locus_tag="OUB02_000175"
     CDS             73995..75437
                     /locus_tag="OUB02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCT8883993.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            75734..76549
                     /locus_tag="OUB02_000176"
     CDS             75734..76549
                     /locus_tag="OUB02_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8883994.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        28754 a         9049 c        13844 g        24939 t
ORIGIN      
        1 ttatagatga tgaagaaata tcaaaatctt atattatagg aagagacact tctttgccaa
       61 aatctataga aaaacaatac caaatccaaa gagaataggc ggagaaaaaa ataaaataaa
      121 ttcaagagta atagaaaagt tttatagaat aaagaactga taactcttta tataacgaaa
      181 aatagaattt taagagaaaa tgatttaata aatgatttaa catggtgttt tagcaggtaa
      241 aaataaatca ccagtaatga ttatagaaaa attactttat gaatctcaaa aaacttagtt
      301 aagagtaaga gatttgataa gataactaaa gttggggaaa tgtgtttgat gagacaaaaa
      361 attagtaaaa tagtataatc tagataagga tattttgttg attaaactac actaatagta
      421 atattgtagg gggaaaagat gagagaagaa tcgaactctt taagtttaga ccatatagaa
      481 attttctttg aacttttatc tcaaagtaca gaagattata tatttttttg ggatataaat
      541 aggaataaat ttaaaatttc ttcagcaatt tttgatgaat ttaacttaag taaagagata
      601 gaatctgatt tagtcaattg ttggagtaaa attgtttatc cggatgatgt tcaaatatgg
      661 aaagatgata tacaagaatt actacatgga aaaaaaggtg agcacaattt agaatatagg
      721 ttgattaata aatataaaga aatagtatgg atttcttgta gaggtaaagt ttatgtgtcg
      781 gatgacccaa aaactatatt tctagttggg agaataaaga atattggtga aaaaaataaa
      841 tttgattcta tcacaggaac gtggaataga gaacaatttg aacatagaat gaattatctt
      901 ataaaagaga aaatttataa aaatggtgct atgtttatta tggatattga taattttaaa
      961 aatataaatg aaaaatatgg acattcttat ggtgacaaag tattgcgtgc aattgctact
     1021 gaagtattag aatatcttcc aaaagatgta agattgtata ggttagatgg agatgaattt
     1081 gcattttttt accctatgtg tactaaagaa actatagaga aaatatatga aaagatacag
     1141 atgtatacaa atactcagca tgaaattgaa tcaaataagt actattgcac agtgactgca
     1201 ggcgttgcta tgtatccaga agatggagat aattatttag atttatttaa acatgttgat
     1261 attgcattgg atatagcaaa aattagtggt aaaaatagaa ttaagttttt ttcacaagag
     1321 ctctatgaaa ataaactaaa ggtaatttca atgcaacaaa aactaagaga gtgcgttgaa
     1381 aataatttta atgactttga gctgtttttt caaccccagg taaatgctgt tacaaaagag
     1441 gtaatagggg cagaggtact tttaagatgg cattcgagta cttatggaga agtatctcct
     1501 gttgaattta taccaatact agaacaaagt aatttaatta taccagttgg gaagtggatt
     1561 ataaaagaag ctgtaaaaca gtgtaaagaa tggcataaaa taaatccaga ctttaaaata
     1621 tctgtaaatg tatcgtatat tcagttaaag gaagattttt ttagagattt tatagtagaa
     1681 tgtttagtag agtatcaatt aagacctgaa tttttaattt tggaattaac agagaattgt
     1741 tggataccag atataaattt actaaatgat aaatttatta gtttaaaggg tataggagta
     1801 tatattgcga tagatgactt tggtacagga tattcatctt taaattacct taaagaactg
     1861 tctgttaata taataaaaat tgaaagaagt tttgttaaga atattacata taatagttat
     1921 gaatatactt ttcttgaata tatcattaaa ttagctcata ttataaattt aaaagtttgt
     1981 gttgagggaa tagagtctta tgaagagtat gatatagtaa aaagcttagg tgtagatatt
     2041 attcagggtt ttttatttgg aagacctgta agtgcctctg aattttataa atttcagcta
     2101 tcaaagtgat gtatataaaa tgagatatat agtatataaa aatagggcaa agtggaagtt
     2161 ttgaagttta ttaaaaattt acactttacc ctattttgtt atgtcttcta taaaggatta
     2221 atatactaca ctaactatta tatactttca ctccaaattt agagattatt tcactagctt
     2281 tttggacatc ttccttagtg gcatcaggaa catcttttag ttcataatcg atacctaaac
     2341 tttcccattt gtgtattccc atagaatggt atggtagcaa ttcaaatctt tctacatttt
     2401 tcaatgtagc tacaaattga cctagttttt ctaaattatc tatatcatca gtaatgccag
     2461 gaactaaaac atgtcttatc catacaggaa tatttcttga ttctaagtgt ctagcaagtt
     2521 ttaatgcctt atccatacca acaccagtca aatcaataga tttttcctca accatatgtt
     2581 ttaaatcaag taaaaccaaa tcagtattat ctaaaactgg gtctatagtt tctatatcta
     2641 caaatccaga tgtatccaag caagtgtgta tatcattttc ttttgctttt ttaaataatt
     2701 cacttaaaaa ttctggttga agagtggatt caccaccaga ggcagttata cctccaccag
     2761 aaaacttcat aaaagaagta tacttaagag catccgttat tatttcatca actgtgtatt
     2821 cttttcctga tttaatatcc catgtatccc tgttatgaca gtatttgcat ctaagaggac
     2881 acccctgaaa aaataatata tatcttattc ctggtccatc tacagtacca aacgtttcta
     2941 tagaatgtat ctttccctta atcatagagt tcacctcaca atatatttta ataaccttat
     3001 caatagttat aatatttctc taaaaaaggc catcttatac cagaaataat tcaaatataa
     3061 gatagccttt tatttttatt ttttacacag tagtaatctt agttaaagta taaaaattta
     3121 aagactaagc cattttaccg tggaatgttc tgtttataac atctaattgt tgctctttag
     3181 ttaattttat gaagttaact gcatatccag atactcttat agttaattgt ggatattctt
     3241 ctggatgttc catagcatct tctaaagtag ctctatcaaa tacatttaca tttaagtgat
     3301 gagcattttg agcgaagtat ccatccatca ttgcagaaag attgtttatt ctttcagtca
     3361 tatcttttcc taaagcagca ggtactatag agaaagtatt agatatacca tcttgagcat
     3421 gttcatatgg taatttagca actgaagata atgaagctaa agcaccatta gtatctctac
     3481 catgcattgg gttagcacca ggagcaaatg gagcaccagc tcttctacca tctggtgtat
     3541 taccagtttt cttaccataa acaacatttg aagttattgt aagtatagat tgagttggat
     3601 aagaatttct ataagtttta ttctttctta ttttattcat aaagctttca actaactcta
     3661 cagctatact atcaactcta tcatcattat ttccgtattt tggataatct ccttctattt
     3721 caaaatcaac agctattcct tgttcatttc taatagtttt aaccttagca tgttttatag
     3781 cagataatga gtctgcacaa actgataatc cagccatacc acaagccata gttctaaata
     3841 tatctctatc atgtaatgcc atttctaatg cttcataaga atatttatca tgcatataat
     3901 gtattacatt taaagtattt acatataatg ttgctaacca atcagtgaat ggttcaaatt
     3961 tactcataac ttcatcataa tctaagtact cagaagttat aggttcaaat cttggtccaa
     4021 cttgaacacc agatttctca tctacaccac catttatagc gtataataaa gttttagcta
     4081 agttaactct agctccaaag aactgcattt gtttaccaat tctcattgca gatacacaac
     4141 aagctatacc atagtcatct ccccagtatg ctctcattaa atcatcattt tcatactgta
     4201 ctgaacttgt atctatagat actttagaac agaaatcttt gaatccttga ggtaatttag
     4261 ttgaccatag aactgttaag tttggctctg gagaaggtcc taatgtatat agagtattta
     4321 acattctaaa tgagttttta gtaactaatg ttctaccatc tattcccata cctcctatac
     4381 actcagttac ccaagttggg tctccagaga ataggttatt gtaatcagga gttcttagga
     4441 acttaaccat tcttaatttc ataacaaaat ggtccatata ctcttgtaat tcttcttctg
     4501 ttacgattcc agcttttaaa tctctttcaa agtatatatc taagaaagta gatgttctac
     4561 ctaaagacat tgcagcacca ttttggtctt taacagcacc taaataacca aagtataacc
     4621 attgtacagc ttcttttgag ttagttgctg gttttgagat atcaaatcca taagattcag
     4681 ccattttttt aagttcattt aaagctgata tttgctcagt tatttcttct cttaatctga
     4741 taacttcctc atccatgcaa gaaacttcta atgatttctt ttgctcattt ttatcttcta
     4801 ttaaagcatc aacaccgtat agagcaactc ttctgtagtc accaattatt ctacctctac
     4861 cataagcatc tggaagacct gttattatac cagattttct agcagctctc atttctggag
     4921 tataaacatc aaaaactcct tggttgtgtg tttttctgta tttagtaaat atatctttta
     4981 tttctgggtc taattcatat ccaaaagctt cgcatgaatt ttcaaccatc tttataccac
     5041 catatggcat gacagctctt tttaaagggg catccgtttg taacccaact attgtttcta
     5101 attctttatc taaatatcct ggagcatatg cagctattcc agaaacagtt tttgtatcaa
     5161 catctaaagt accaccattt tctctttctt ttttaaatag gaccattgct tcgtcccata
     5221 attttttagt attttcagtg gcaccagcta agaaaaagtc atttccctca tatggagaat
     5281 agtttaattg tataaactct ctaacattta tttctttagt ccatcttcct gttttaaatc
     5341 cttgccatgc attcattaag tatttcctcc ttagatttaa atgataaata atactattaa
     5401 aattaatttt tatatactta atttatttta tatatttttt taaaaatata ttttctaata
     5461 taataatagc atattattcg tatcctgtat acataaaaaa taaaaaattg tcgctttaaa
     5521 gtataaaaca gattatatgt taataatgtg gtgaaattat acccatatat aaataaaata
     5581 aaacaaataa atagctaatt aaggagaaaa tattttttat tttttgttta ggataaataa
     5641 attttattat ggaaaaaaga aaaagcatac atcttcacaa actgaatatt tatttaatag
     5701 ggggaggtga tagattgttt atcacagtgg tattattttt agtaggtttc ctattaatta
     5761 ctaaaggcgc agatatcttt ataaattgta ctgtagagat aggtaaaaaa acgaatatat
     5821 cagaaattat acttggagct actatagtta gttttgcaac aacattacct gagtttacag
     5881 tgtctctact tgcttctatt gatggtcata ctactatgag tttaggaaat gcagttggtt
     5941 ctataatatg taatacaggg ttagcattag ggttggttgt ttttataagt ccatttaatg
     6001 tagataaaaa aatgtttttt tctaagtctt tactattaat agtatccgtt atagttttaa
     6061 ttttattgag tttagatgga gttataacaa gaggtgattc attattactt ataataatat
     6121 taatctttta tatggttaat aattatagaa gtgtcgttgg aaaatctgat actaaaaata
     6181 gaaatattaa aaataacact agtaaagaca tatctactaa aaaagggaaa aattataggg
     6241 gtttttccgt attagaaatt gtaaaaatac tattactttt tgctacagga ttgataatga
     6301 tgataatagg ttcacaaatc cttatagaga gtggagtaat aatagctagt tttttaaata
     6361 tacctcaggg tatagtaagt ttaactataa tagcacttgg aacttctctg ccagagatag
     6421 tttcgtcgat tactgctata agaaaaaatc atcatgaaat atcagtagga aatattttgg
     6481 gtgcaaacat attgaatata gtatcagtaa tagctgtatc agcaatacca aataacatac
     6541 caatactatc tcaaaataga cagttggata taccattcat gatattatta ttattaatag
     6601 taataatacc aacacttaaa tctaataaac ttagtagaat acaaggtata ttgatgttat
     6661 tcacatattt tctgtacata tcaattttgt atttcatgta tattatttaa ctatactgtt
     6721 taaaatatta attgcagaat atcctaaaaa agtatataat ggataggtta agattaattg
     6781 ataacggaga ggaataatat atgaatataa caaagtttga agatttacca ataagtgagg
     6841 gaataaaaaa agctatagct gaaatggggt ttgaagaacc atctcctata caagcacagt
     6901 ctatacctgc gattttgtca ggtaaagacg ttataggaca agctcaaact ggtacgggaa
     6961 aaacagctgc ttttagtatc cctatactag aaacaataga cccaaataat agaagtttac
     7021 aagctgtagt gctttgtcca acaagagaac tggcaataca agtatcaaca gaaataagaa
     7081 agttagctaa gtattcgcac ggaataaaga cattacctat atatggtggt cagccaatag
     7141 atagacaaat aaaatctcta aagagtggtg tgcaagttgt aataggtaca ccaggacgta
     7201 caatagacca tatcaatcgt aaaacattaa agatggataa tgtaaaaatg attatattag
     7261 atgaagcaga tgaaatgcta gatatgggat ttagagaaga tatagaaatg attttaagta
     7321 agattccaga agaaagacaa acaactttct tttcagctac tatgccaaga ggaattttgg
     7381 aattaactaa gagatatcaa aaagacccag agcatataaa agttgttaga aaagaactta
     7441 cagtttcaaa tacaaagcaa tactatatag aaactaggtc atcaaataaa ttagaagtat
     7501 tatgtagatt agtagatgta tatgacccta aactatctgt tgttttctgt aatactaaga
     7561 gaaaagcaga cgaattggta ggagatttac aagcaagagg atattttgca gatgcattac
     7621 atggtgactt aaaacagact caaagagata tagtaatgga taagtttaga aatggtacaa
     7681 tagatatact tgttgctact gatgttgcag ctagaggtat agatgttgat gatgttgaat
     7741 gtgtattcaa ctatgactta ccacaagatg aagagtatta cgttcataga attggtagaa
     7801 ctggtcgtgc tggtagagaa ggtatgtcat ttacttttgt atttggaaaa gaaatgagaa
     7861 agatgaaaga tatagaaaga tatactaaat ctaaactaat aaagcataat ataccaacaa
     7921 taactgatgt tgaagagaaa aaagtaggta ctttctttgc acaagttaaa caaactatag
     7981 aagaaggtca cttaactaaa caattacaat ggttagaagg tttctgtaat gatgaagatt
     8041 atgcaatggt tgatatagct gctgcacttg taaaactatc tctaggtgaa gaaatgaaag
     8101 aagaaataat agaagaaaaa cctagaagag agcgtggaga tagaaaaggt ggtactggag
     8161 ctaaagatgg tatgattaga ttatttatca atattggtag aaatcaaaga gtacaagcaa
     8221 aagatatagt tggtgccata gctggagaag ttggaatacc aggtaaagta gtaggtacta
     8281 tagatatata tgataagtat acttttgttg aaatacctaa aaaagatgca aaaacagtaa
     8341 ttgaaaagat gaaagatatt aagataaaag gtaataagat taatatagaa aaagcaaata
     8401 agaagaaaaa ataacgtata gtatacaaaa ctataattag aaaatatata aaataatata
     8461 ttggatattt aaaaagcctg atgtgttttc aaaacagata gtagataatc tgtaagagtt
     8521 aacagcataa ggcttttctt gttttatatg atgttataat tttccagatt attgtgaaaa
     8581 atgtatatta gttccttttt ttcaaatctg atttgaaaaa gagaatatta attatctata
     8641 tagtttagat tataataaaa aatgtaagat aacaaagggg agaaaaagat gtcaatgact
     8701 tatatggacc ctagggtttt tttgatttta gaaatggtgt tagaagaaga cacaaatact
     8761 ctagaacaat tttcaaatgt tttgggtgtt agtactagga ctatcagaaa ctatataaac
     8821 cagattaatc gagaattaaa aggtggtatt gctaaaatag ttaaaaatga aaaaggtaca
     8881 tatagtataa gaattgagga tgaaagtaaa ttaattgaga ttgtaaattt taatagagga
     8941 aagctatcaa attttataaa tttaaattca cctgatgaaa gaataaatta tgttcttgat
     9001 attttaacta tgaccaacaa tccaattacc atagatgatt tggctgaaga gatatgtata
     9061 ggaagaacaa cattaataaa ggatttaaaa aaagcagaca gaattttagc tgaatatgat
     9121 ttagaattaa aaaggaagcc aaatataggt atgattttag ttggtgaaga aatggatata
     9181 agattattaa tccttgatag attgtatgaa aattatattg atgtcttgga aaacgttaat
     9241 cagataaatt tagttaaaga ttatgatatt gaatgtctta gagatgaatt aaaaaagtta
     9301 tttaaaaaag aagaactcta cataacagaa caggtcttga gagatgttga aagatatata
     9361 atcatgtctg tcttaagaaa tctaaatggg tacaaatttg aaaagataga taagagattt
     9421 gaagtgataa gatgttcaga tgaatatact ttaggattaa agctcaaaga tttattggaa
     9481 aagatattta atatagtttt aaatgaaaaa gaaaccatat ttttgacaat gccattaata
     9541 ggtagaaaag ctcctagtac aaaattagca cttagcagta taaagataaa tgatagcgta
     9601 aaggaagttg tagatgaagt aactagtttt ttattagaga cctcagggat tgattttaaa
     9661 gaagataaaa agcttataga aaatttggaa tatcacttgt attttgcact taatagaatg
     9721 agattcaaca taagggtaaa aaatcctctt ttacaagaaa taaaggaaag atatacattt
     9781 ccatatagtg ttgcaaagat agctgcaatg ataattgagg aaaagtttga cttgaaaata
     9841 tgtgaccacg aaataggata tatagcatta cattttggaa gttattttga aaaaaattca
     9901 agaaaagttc taaatgtaaa tagggtagct gttgtttgtg gaacaggtct tgggactgca
     9961 caacttatta gaataaagat aggaaaaata cttggagaaa gtgtagaagt aaatactttt
    10021 tctgatatag aagctaaagc taatttagat ttattaaagt attatgattt agtatttaca
    10081 acagtagatt tcaaaacaga cttagatgtt ccaattttta gagtaaatgc gttatttaat
    10141 gaagaaagta taaagaaaga gatagaaaat gcaatacttt tgaaaaatag tataaatctt
    10201 aagtctataa aaaagattga aaaaaatatc ccttttatag gtaaattaat tgaagaggat
    10261 aagttcttta tattgaataa gagtacgttt atggaaaatt tagaagatat gttagaaaaa
    10321 ttagttcata aaggaattat tgatatagag ttcaaaaaaa aggtattaaa aagagaaaag
    10381 aaatcttcaa cagcattaga taattatata gcattgcctc actcagttaa tactaatggg
    10441 gaaaatcttt atctggcatt tggaatatta gaaaaaccag tgatttggga tacaaaggaa
    10501 attagaataa taatattgat gataattcca gataagaatg tagatagtac agacctgata
    10561 ataaaaagtt atgaagaagt acttgaagta gggagagata aaaagatggt agaagaactt
    10621 gctaaggcaa aatcatttga tgaattttat aaaataatta ccaagaggag gtaataatat
    10681 ggacccagta atttttatta tagcatcact tattacagtg tatttattaa atatttttct
    10741 tggatactta cagcttaaag attttaataa aaattatatt gaactaaaaa gaaaaggaag
    10801 agtggcaatt ggcagaaaaa aaggaagaat ttcatcgggt acaattgtgt tgatacttat
    10861 aaatgatgat ggtgtgattg tagagactag aaaaatgcaa ggtgttacag tattggcaag
    10921 agtaaagtca tttgaaggtt tggtaggaaa gagcttagag tatatagaaa aatcagattt
    10981 aactgtttac aataaactac ttaaaaaagc aatactagat gcagtcaaac agtacataac
    11041 atttgagaaa aataaatgtg aagatacaaa taatctgagt actgatgaag agtgtaggat
    11101 ggaagtagtt taaaaaaata tatttataag gaggagttaa tatgacgcaa ttttttcaag
    11161 tattagcaag tggtgcagat agttttatga atttatttag ggcaggtggg caacaatttg
    11221 taggttttgt aacagatata gtaccacttt tagtagcatt attagttaca atgaatgcaa
    11281 ttatcaactt tattggtaca gatagagtcg agagacttgc aaaaaaatgt tctgcaaata
    11341 tatttactag atatttgata ttaccagtcc ttggtacatt tgtatttgca aacccaatga
    11401 cactatcatt aggaagattc ttgccagaaa agtttaaacc tagttacttt gcagcagcaa
    11461 gcttttcatg ccatacaatg aatggattat tcccacatat caatccaggt gaactattta
    11521 tatatctagg aattgcaaat ggtataacaa ctttgggatt ctcaacagca gatttagcag
    11581 ttagatattt gttagtgggt atagttgcaa actttattaa aggtgtagtt actgactata
    11641 caacaaaatt tgtagagaaa caacaaggaa tagcattaaa ttcagatata aagattggat
    11701 aattacttta aggtataaat aaatttttca aataaaaaga tagtgtatag aggaggataa
    11761 attatatggc atttaacaaa gttaaaatag taaaaggttc tggaggatgg ggtggaccat
    11821 tgataataga accaacagaa aagaagaata aggtagtata tgtgactggg ggagcaaagc
    11881 cagaaactgc agtaaaaata gctgaactta caggatgtga gttaatagat ggattttcac
    11941 agggtgttaa agatgatgaa atcgcatgtg taataataaa ttgtggtgga acattgagat
    12001 gtggtatata tcctcaaaag aaaataccga ctataaatat aatgaaaaca ggaaaaagtg
    12061 gaccattagc aatgtttata aaagaagata tatatgtttc aggggtaaaa ccaacaaatg
    12121 tagagcagtt ataattgagg tagtaggagg gaagaaatat ggcttatgat actaaaaaga
    12181 agataagtga gcagagtcaa ggaattgttg caaaaatagg tatagcacta ggaaatgtga
    12241 caagtactct ttttcaagct ggtagggata ctatagatac attaatacat actatactgc
    12301 cttttatggc tttcgttgca gtcctaatag gtataataaa tcaatcgggt ataggaaatt
    12361 gggtagccaa tttacttact ccattaatcg gaaatgttgg aggtttagtt gtcttatcaa
    12421 taatatgttc atttccgttg ttgtctccgt ttcttggacc aggagctgtt atagctcaga
    12481 tagtgggggt attggttggt acagaaatag gaaaaggaaa tattccacca agtttagcat
    12541 taccagcatt gtttgcaata aactcacaag cagcttgtga ctttatacca gtaggtcttg
    12601 gtcttgcaga agctgaacct gatacagttc aagtaggagt tccgtcagtt ttatattcta
    12661 gatttttaac aggtgcacca actgttctat tagcatggtt agcaagcttt gggttatata
    12721 aatagaagaa tactttataa ataattagag agaggttcag ttgatatgga attaatatac
    12781 agtactcaaa taaataaaat aggagataat gcaaatgaat ttttacaaca taatatgttt
    12841 gtaactttca aagataatgc tcctcaagag ttagaaaatt actgttatat acattctgaa
    12901 aataatttag taaaagaaat acttacggat gatgtgctat caataaataa tgttgattac
    12961 aaaattactt ctgttggtga gttagtaaac caaaatcttt cagaattagg tcatataaca
    13021 tttaagttta caggtgaaaa agatggagca attggaggaa ccttatatct tgaagaaaaa
    13081 gaaatagcac ctattggcgt aggtactata ctaaaaataa ttagaaaata aaatttaaaa
    13141 cgaatctaat catattataa gtaaattata gataaaagtc aatttaacta atcaagttaa
    13201 cttttaatgt gataaaaaag gggaaatgaa aatgagaaaa gtggctttag tcgttggagg
    13261 aggaagaagt ttaggtgaat atttatctaa acatttagct aaagtaggtt atgatgttgc
    13321 tattgcagat ttaaatgaag acaacgcaaa aaaagtagct gaagaaataa aaactgaatt
    13381 tggttgtaaa tctgtaggat atagttgtaa tgccatagat gaaagacagg ttataagtac
    13441 tgtcaaagct attgaaaatg attttgatag aatagattta ttagtgtaca atgcaggagt
    13501 ggctgtaagt aaaaaaataa ctgattttgg atacaaagat tttaaattta ttgtagacgt
    13561 aaatttaaat gggtactttc tatttgcaag agaagtatct aaagtcatga tagcaaaagg
    13621 tattgagggt agaataatac agataaattc aaaatcaggt agagtgggaa gcaagcataa
    13681 ttcaggatat tcagcaggta aatttgctgg agtaggactt actcaaagtt tagcgctaga
    13741 tttggcagaa cataagataa ctgtaaatgc actgatgctc ggtaatttac ttgattctga
    13801 tatgtttgaa agtttaattc ctcaatatgc agtaaaattg ggaatacctg aatcagaagt
    13861 caagcaagtt tatatagata aagtaccttt aaaaagaggg tgtagattta tagatgttgc
    13921 aaatatgctt acattttatg cttcaaagga agctgaatac tgcactggtc aatctataaa
    13981 tattactggt ggacaagtta tgtaaggttt ttatggagtg tctcaagatg aactctttag
    14041 ttcatgaggc acttttttgt ataaaattaa atatattttc agcatttaaa caatgaaaaa
    14101 gaggtttatc tttattttga gatagcctca tttttgtaat taaaaaacat agtgtttaag
    14161 taaacatata agattgttag ttttttaaaa gagaattttt taataatatt atatttatga
    14221 agtattttta aacttaattt gataaaatat agctatgttt tatatactgt agttttgaac
    14281 atagaaagtt taataaatta tagtatatat gttaattttt aataaggtaa gaaagtagaa
    14341 agaaggatat tttatggata taataaaaaa attaagtcat gggtgtacat tagattgtca
    14401 tgactgttgt aaattcaatg tttatactaa aggaaataat gtagtaaaaa ttgaaggaga
    14461 taaaaatcat ccttatacaa aaggttttat atgcaaaaaa gggatggccc atttagatag
    14521 attaaatcat aaagatagaa taaagactcc tatgcttaaa gttgatggag tatggaaaga
    14581 gatttctttt gataaagcta tagaaataat ggcagaaaag cttacatact ataaagaaaa
    14641 gtatacatca aagtctgtca tgcattatga ccaatatgga agtgggtctg tgctaaaata
    14701 cataggagat atatttttta atttttatgg aggtgttagc agacataagg ggggtccctg
    14761 ttggagtgca gggatgcatg ctcaaaaata tgattttgga gtagctaaga gtcatgctat
    14821 agaagatatg ttaaatagta aaagtatatt tgtttgggga aaaaatccag catatactac
    14881 aattcatact atgcaaataa ttaaaaaagc taaagaaaaa gggataaaga tagtagtgat
    14941 tgaccccata tatacaaaaa cagctcaaat agcagataag tatgtacaag taaatcctgg
    15001 aacagatgga gctttagcta tagctatggc aaaaattata gtagaagata aattatatga
    15061 tgaagaatat ataaactcgt atgttattgg ttttgaagaa tataaaaaat atttatcttc
    15121 attggaatta agttttttaa ttgatgaatg tggtgttaaa gagaatgata taagagagtt
    15181 agtggattta tatactaaca aatattcaag tataaatgtt ggatatgggc ttcaaaaata
    15241 taaaaatggt ggaaacacaa taagagcaat agatgcttta ggtgctataa ctggtcaaat
    15301 aggatttagt ggtggagggg taaactatgc taataaagtt tatccgagtg ttataaattc
    15361 tgacccttac aatagccaat catatggtga agatagggag ttttatgtaa gtaatatcag
    15421 taaatttata gaagaatctt taaaaaatac atcaaataaa gtaaattatg catcagatga
    15481 acttgatatg acatcaaata aagtaaatta tgtatcagat gaacttgata taacatcaaa
    15541 taaaacagat tatatatcaa atgaattgta taatttatct aataagtcaa tcaaagataa
    15601 tattcctata aagatggctg tgataactaa aagtaatatg ctgaatcaac tgccagattt
    15661 agtagaattg gagagggtgt tttcaaaaat tgaatttaaa gtatgttttg atatgtttat
    15721 aactgataca gctactttat gtgacatgtt tataccatgt acaaatacac tagagagtga
    15781 agatataatt tatagttcaa tgaccaatcc atatataacc tataatgaaa gagctgtaaa
    15841 gccagcacat aaattgatgg atgaatatta cttctttatg gagctagcta aaaagatggg
    15901 attaaatgat tatccttttg tagaaaagag aacttattta gaaaaagtga ttgaaccact
    15961 taagcgtttt gacaaaaatc tggatataga aaaacttaaa aacaattact ttactataca
    16021 taaccctgtt gcttgggaag ataagaagtt tgagactcca tctggaaaat acgagcttta
    16081 ttcagaaagt ataaaaaact tgggaataag tccaacacct gtatatataa gcaataaata
    16141 taaagaaata gaagataaaa atatttcttt tagattatta actaatcatc atgcagatac
    16201 tttatttagt cagcatttta tggacaaaaa atctatagct caagcatata taaatcggag
    16261 aatggctaaa aaagtaggta ttgaggacaa agatattgtg atattgaggt caaaaaaagc
    16321 aaaaataaat gtacaaataa atatagatga tggagtaggt aattatatag taaaaatgta
    16381 tgttggttgg tggaagaaac atggaaatcc taatagttta acagacactg gtatatcaga
    16441 ttttgggggt caggtaacat ataatgaaag tatggtagaa ataatacgac aaaactagtt
    16501 aaatttcatt gaaggaataa aaatataatt atagaattga ttaaaagtta ctatttttac
    16561 aaaactattg aaggagggat ttttgatggt aaattattcc ttagaacata agaatctata
    16621 catagaattt atgtgctcag aactagacca ccatgtagcc aatgaaataa gggaagaaat
    16681 agataactta ttaagtgtaa atcaagtaaa aaatgttgta tttaattttg aaaatattaa
    16741 ttttatggat tcttcaggta taggcgtcat cataggtaga tataaaaaga tttctaatga
    16801 agggggacga gtttcagtaa ttaatataag ttcacgagtt aagaagattt ttgatttatc
    16861 tgggttaaat aaaattattg gcatttatga tacgtatgaa gaagctctaa gttctttgta
    16921 gggaggagta tgtgatgaat aatattatgg aagttaaatt ttcggcaata tctgaaaatg
    16981 aaagtttagc aagagttata gtagcatcct ttgcagctaa gttagaccca acagtagatg
    17041 agttagtaga tataaaaact gctgtctctg aggctgttac gaatgctata atacatggat
    17101 atgaagaaga cagttctaag tttgttttct taagatgtga gatagaagga aatactataa
    17161 agattatagt tgaagatgaa gggtatggaa tagaaaatgt ggagaaggca atggaaccac
    17221 tttatacatc aaaaccagaa cttgatagat caggaatggg attcacagta atgaaaagct
    17281 ttatggacga tgtagaggta agttctgtaa aagataatgg gactagaatt gaaatgacta
    17341 aaaaaataaa tgtgcctaaa taaagtccaa aagtgaggtt ttattatgga agtaactgtt
    17401 gccagagaag aaaaaaaacc tcttcttagt catgaagaga ctctagaact tattgaaaaa
    17461 gttcaaaatg gagatgaaga ggcaaaagaa atattaatat caagcaattt aggtctagtt
    17521 aggagcgttg tatctagatt tttaaatata ggatatgata gagaagattt atttcagtta
    17581 ggttctatag gcttaataaa atcaatatat aaatttgacc caaaatttaa tgtaaaattc
    17641 tctacttatg cagtaccaat gattttaggt gaaataaaga gatacttaag agatgatggt
    17701 atgataaaag tatcaaggtc gttaaagcaa atagctgtaa aggcaaagat ggaaagtgaa
    17761 gctttaacta aaaaattagg tagagagccg agtatagaag aattagctaa agcaatagat
    17821 gtagaaaaag aagatttagt aatggctatg gaagctaatt tcaatgttga gtatttgcaa
    17881 ggagttattc atgaagagga aggttctcca atatgcctta ttgataaaat aagcatgaaa
    17941 ggagaaagtg aagaagaaaa agtagtggat aacatattgt taaaagacat acttggaaga
    18001 ctggataaga gagagcgtca aataatagtt ctcagatact ttgaagacat gactcaaagt
    18061 gaaataggag agatgctcaa tatatcacaa gttcaagtat ctaggataga gaagaaagta
    18121 ttgtctaagt taaaagaata tatatcgtga taatacttat gatatgtaga ataacaaaat
    18181 ataataaata tattactaaa ataaaatgta ataacttttt atgttaagtt tttaaattat
    18241 taaaagatat taaaataata aaaagcatta aaaaagtaaa aatataaaaa caaggtgtct
    18301 ttatggaaaa atagattttt tctttattag acatcttttt tattttttga ccaaacttta
    18361 gatagtcaaa ataaataata ggtttatttt tttaaaatta acaaataata ctttagagag
    18421 gtgtttagta tggataaaaa ttataaaaaa tatgtagacc aaataagccc aaaacctaca
    18481 tatttaaaaa actatacact tgcatttatt gtaggtggaa ttatttgtat gatagggcaa
    18541 gcaataaatg atttgtatat gaaagttgga ggtcttgata aactaggtgc tagttctgca
    18601 acatcaataa cattaatatt tataggggca tttttaactg gattaggagt ctacgaccta
    18661 ataggaaaaa gagctggagc tggttctata attccaataa caggttttgc taactcaata
    18721 gtatctccag caatggagta taaaagagaa ggctatgtat taggtgtagg tgcaaactta
    18781 tttaaaattg caggaccagt tttagtttat gggataggtt cttctatttt atgtggaatt
    18841 atttattata tttttaagat gttttagatg atttttattt aatttaaatt ttattaatta
    18901 tgaagaaggt ataaaatctg aaaatagcta ctaaagtaac taattgaaaa ataattgtgc
    18961 agatttaatt aaaattttta attaatatta aaaatttgaa tagtaaaatg caaaattaaa
    19021 aaggtgtgaa aatatgaaaa ataaaagaat tggaaaaaga acagtcaagt tagaaaataa
    19081 accaaccata atatcaactg gaactatagt aggtcctaag gaaggtgaag gaccactaaa
    19141 ggactatttt gatatgatta tgactgacga tttatatggt gaaaagactt gggaattagc
    19201 tgaaagtaaa atggtagaga ctgcatctca acaagctatc caaaaggcag gtaaaaaatt
    19261 gtcagatgta aattatatgc taggtggcga tttaataaat caaatagttc cagcatcatt
    19321 tgcagcaaga gaactggcta ttccattttt aggtatatat ggagcttgtt ctacaatggc
    19381 agaaggtctt tgtataggtt ctatgcttat agatggtggt tttgctgatt tagtactgtc
    19441 aggtacatca agtcactatt gtacagcaga aagacaattt agattcccac ttgaacttgg
    19501 aaatcaaaaa ccaatgacag cccagtggac agttacagga gcaggaagtg tattattagc
    19561 tcctaatgga gacggtccaa aagtaaagta tgttactgtt ggtaaagtaa tagatgaggg
    19621 tattgatgat ggtaataata tgggaccagc tatggctcca gcagcaatag atactatcta
    19681 ctcttacttt gaagatacta atgatgaccc aaatagcttt gatataattg ctactggaga
    19741 tttaggtact cttggaaaac agatagcaga agactttcta aaagaaaaag gtgtagatat
    19801 atcaaaagtt tatactgact gtggtataga gatgtttaac cttaaagaac aagatgtcca
    19861 ctgtggtgga agtggatgtg ggtgttcagc tacagtgttt gcaggatata tatatgataa
    19921 gttgagaaaa aaagaattta acaaagtaat gcttgtatca acaggagcac tactttcacc
    19981 gacaagtaca ctacaaaagc agacaatacc atgtgttgca catgcagtag taatagtaaa
    20041 tgagtaggaa gtgatataat atgttaatgg actatgtaag agtatttatt gtaggtggaa
    20101 ttatatgttt aatagcccaa gtaatgatgg attatttcaa aatgcaaact ccatatgtta
    20161 tggtaacgta tgtaacaact ggtgtagttt taacttttct aggactttat gagccattag
    20221 ttaagtttgc agcagcaggt gcaacagttc ctataatagg atttggatat tcacttgcta
    20281 caggagtaat aaaatcaatt caaagtgatg gttttttagg tattttttta ggaggtacaa
    20341 ctgctacagc tggtggaata gcagcagcta tattctttgg atatattatg tcagtagtat
    20401 ttactccaaa agcaaaacca taaatcttta ttaaaagaat aataagtttt tgaaataata
    20461 actttttata aagcaggtgg atatctttta gattccacct gttttttata taaagaagcg
    20521 tttacataat tgaagttgac aattatttac aacatagtgt agtattatat tatcaaaata
    20581 aaatgaagtg aagtagggga taaagatggt aactattaaa gatattgcaa ggaatctagg
    20641 tatcagttat tcaactgttt ctaggtgctt aaacaataat cctaatgtat ctgaaaaaac
    20701 aaaaaagaaa gtggtagaag aggcaaatag actagggttc cattttaatg taaatgcaag
    20761 aaatttagca aaaaaagaaa ctaacaggat aggggtaatc ttttcaaata attttaatca
    20821 tcaagatact agaaagtttt ttagtgatat aatggatagt tctataaatt ctattgagac
    20881 taataaatac gattttataa tacagcctaa taataacatt agtggtgata gtaatgtata
    20941 taaaatggta aatggacaaa tggttgacgg attagtaatt gtgtctcaat caataaaaaa
    21001 agaagagtat gattttttaa aagacaataa ttttccacat gtatttatat attttaaacc
    21061 atcttttata ggagaagtag ataatttctt ttgggacgat aacgtgtatg gaggttatat
    21121 agctaccaag catttaattg accatggtca taaagatata ataacagtaa cttcagatga
    21181 taagagttca aaaatgcatg aagatagaac caagggatat ttgaatgcta tggaagaagc
    21241 aaatttaaag acagaagtca ttaaatctaa aatggatttt gaatctcaag ttgaattttt
    21301 agaaaaatac attgataaaa taaaaaaagc ttcagctata tttgtacaac aagatgtacc
    21361 agcgatttct ataatacaag agttaaaaac tacttatggt ataaatgtac cagaagatat
    21421 ttccataata gggtataata atattgaatt aatatcatat tttaggtcgc atttatcaac
    21481 aatagatgac ccaagagagc aagttataaa aaatggagta gatagtttag taaatataat
    21541 aaataaaaag agtacagaac acattccaag aaaattatat ccaaggttaa taatacgaaa
    21601 tagcgttaaa agaataaagt agtaagcttt taagtatcaa acgtttatta aaaatattat
    21661 agattatgtg aagtttttaa caaataatat aaataaaaat taaaaggagt aggtgtatat
    21721 aagtacattg tactccttta tttattgaga tagtttttat aatatgaatg aataatttta
    21781 ttcaattatt tgaaaatccg ttattttaac aatataaatt aataaattat ttgtatttta
    21841 tatcatgaat ctataatatt tttttgattc ataactaaaa gtatataaaa atgatgaata
    21901 aatatatatt atacattatc aatgataggt tatataataa aatcactaac aattatatta
    21961 taagaaaatt ataaaaatta caaggaggta tagaaatgaa agaaaaagca gggattagaa
    22021 atgccataat agcagccttt ataatcacaa tattggcaga attaattgga cctatttcat
    22081 ttaaagttat gggaattaat gtaactttac ttagtatact atgggctata ttcataggaa
    22141 tggctgtttc tcctcattta ttagggagag ttataccagc cttaaaaaag tttatagggg
    22201 ataatgaaat aaatgtatct ccatatttat tatctttgac actatatcca ttagggataa
    22261 tgtttggaat taatgcaggg cctaaagttg gtatattact tcaagcagga ccagcacttt
    22321 tattacaaga atttggtcat atgggtacaa tgttgatagc attacctgtt ggagtagcat
    22381 taggtcttgg aagaagtgca ttaggtggta ctttttcatt atgtcgtgac acagcactag
    22441 gaattattgg agataagtat ggattaaact caccagaagg tataggaaca ctaggaactt
    22501 acataagtgg tagtatattt ggaacattat tattttcatt tttagcacca gtgggtgtac
    22561 ttttaggatt tcatccatat gctttagcaa tggcatcagg aatgggtagt ggttcaatga
    22621 tgggagctgt aacagcatca ttaatgaata cagttccaca aatgtcagag cagattttag
    22681 catattcagc tactagtgga ttgattacag ctgttactgg aatatatata gaattattct
    22741 tatctttacc agtagccaac tactattata gcaaagtagc tccagttatt gaaagattta
    22801 gaggaagaaa aacaagaaga acaagtaata aattgtaaag gaggatatat atatggaaaa
    22861 aacttttaag actattatgg agcagataaa aatagtattg tttatatata ttattatttg
    22921 ctttggacaa atgatagcac ttaagactca ggctcaagat ttgttgattg gaagtgtatc
    22981 tggatttttg ttggtgataa ttgctattat aataaagaat tttgttaaaa aacctaattt
    23041 accaggtttt gcatgggcta cattagttgc atttgtttta tcgcttccaa tatctccagt
    23101 gggggatatc atagcaacaa atgtagggaa aatcaatttt atgtctacag taacaccatt
    23161 attagctttt gcaggaatct ctgtaggtaa ccaattagat atattaaaga ctttgagttg
    23221 gaagttagta gttatatcat ttatagttat gacatctact tactttggtt ctgcttttat
    23281 atctcaaaca gttttaagat taaatggaat gctttaagga ggtagtaaat atgaatgaat
    23341 taaaagcaaa aattaaagag gaaataaagt tattatctga agagaaaaag acatcatttg
    23401 aaaaagtttc tgactattta ttttcaaatc cagaattagc atttgaggag tataagtcac
    23461 aaaaagcatt atgtgatttg ttagaagaaa atgaatttaa tgtaactaaa ggtgtaggag
    23521 gattagaaac atcttttgaa gctgtgtatt caaatggaac taatgggaaa acagttgctt
    23581 ttttagctga atatgatgca cttccaggaa tgggacatgc atgtgggcac aatatcatag
    23641 ggacaagttc tgttggtgca ggtatcatat taaaagaaat catgaaaaaa cataatatag
    23701 aagggactgt aaaggttttt ggaacacctg cagaagaaag agtaggtgga aaaataacta
    23761 tgattaaaga aggtgtattt aataatgtag atgctgcatt aattttacat ccaagtgatg
    23821 cttcaatgcc tgatgatata tcatttgctc aagttaattt aaaatttgat tttactggta
    23881 aggcatcaca tgctgctgct tttccttggg aagggaaaag tgccttaagt ggagtaatag
    23941 cactatttaa cagtgtaaat agcatgagat tacatcttaa agattatgca agagtacatg
    24001 gaattataac tgatggaggt agcatacata atataattcc tgaaaaatct acagctatat
    24061 ttaatgtaag agctttaagc atagaatatt taaatgagat ttgtgaaatg cttaaaaatt
    24121 gtgctaaagg agctgcaatt tctactggaa ctaatgtaga aatagttcaa ttagatgaaa
    24181 tatacaaaga gattaagaat gattctgaat tagtgaatat agttagacaa aattttgaag
    24241 ttttgggtga agactatgtt gaaagagatt taagtcaagg tataggttca actgatacag
    24301 gaaatttaac tcatgagata cctgcaatac aagcatatat aaaattaaaa gaaaatactg
    24361 caactcatac agatgaattt gctgttgcag ctggtggtga agaaggcaaa gtagcactta
    24421 taaaagcaat taaggtgcta gcaatgtgtg gagtagatat tttgtatagt aaataatata
    24481 taattttaaa atatattaag aaaggaaatc agattagaca tgatattaaa acaagtaata
    24541 gaggtttatg acattttaga taaagcaaat gctaatgggg aagaagtaaa aacttactta
    24601 caaggttatg gtgaagtaga tgtaactgta aaagaattgt cttcaagtaa aggctcaaca
    24661 gatttagtaa agcttacaat tcctgggaaa aatggaaaat taaaaggtgg agatgctcct
    24721 actcttggca ttttaggtag actaggaggg attggagcaa gaccagaagt tataggtttt
    24781 gtttcagatg gtgatggagc attggtagca attgcagtgg ctgctaaact tttggatatg
    24841 caacgaaaag gagatatatt agatggtgat gttgtaattt caactcatat ttgcccagat
    24901 gctccaacta aagaacacta tcctactcct tttatggatt ctccaattga tatgatgact
    24961 atgaatgaaa atgaagttga tagtagttgt gatgcaatac tttctataga tactactaaa
    25021 ggaaatagag taatcaatac tagaggattt gcaatatcgc caactgttaa agaaggctac
    25081 atattaaaaa ctagtgataa ccttttagat attatgcaga ctgtaacagg gaaatctcca
    25141 tttgtattcc ctttgtctat tcaagatata acaccatatg gaaataatct gtatcactta
    25201 aatagtattt tacaaccagc agtagctaca gatgcaccaa tagtaggagt agcaattaca
    25261 acagagatgc ctgttgctgg atgtgctaca ggtgcaacta attttagtga tttagattca
    25321 gctgggagat ttgcaattga agttgccaag ctatttggta gaaataagtg tgatttctat
    25381 gataaagaag agtgggaaat gcttattaag agatatggca aattaaatag atttcaaaca
    25441 tttggtatac agtaattaga aaaatattaa gtttatataa tttgtgatat aactatttaa
    25501 gaatatataa acaattagga ataaaagtaa gaataaagca cttagttaaa aactttaaaa
    25561 ataaaatatt tatttgttta tataaaaaag gaggattctg gttattggga ttctcttttt
    25621 tatgaataca aataatatat tgtaattata aaaaaaggag cgtttcaaag tgattttttt
    25681 aatcaccttg agacaactcc ttatattttt atttaaattc tattttttat taagttgctg
    25741 gagttccacc accatcagga ggagtagtag agccaccacc attatttcca ccaccatcag
    25801 gaggagtagt agagccacca ccattgtttc cgccattatc aggaggtgta gaaccgccac
    25861 caccattatt tccgccatta tcaggaggag tagtagagcc accaccattg tttccgccat
    25921 tatcaggagg tgtagaaccg ccaccattat cacctgtgtt attattgtta ttattatttt
    25981 cttctggttg gttattgtct ggtttttttg tatcttcaga tgatggttga gaacttactt
    26041 tagtaggagc agtaccttca ataaatgctg ctttagagcc agaagattgc tttcctccat
    26101 cctctaaatt aatttttgta aaatatacat ttttaggaac ttcaaattca gtaaccgtta
    26161 aattagcatg tattttttcc ataatttttt cccataattt tgcagtgcta gtacttccat
    26221 gtggtacttc tctacgtttt atagtgttac cagaatcatc ttttctacca gcatcatctc
    26281 caatataagt tgcaccaaca taataaggag tgtaaccgac aaaccaagca tcatatgcac
    26341 tatttgtagt acctgtttta cctgctacag gcattccctt tggaagtgca gcaacaccac
    26401 cagtaccttc agtaattaca ctttctaaca tatctgtaac gacataagct acttcaggtt
    26461 ctacaacttt atgttcctca ggagcatttt taactaataa ttgaccatca aaggtagtta
    26521 tagtagtaaa aatagaaggt tctacataaa taccgtgatt tgccaatgta ccataagcag
    26581 cagccatttg aagtggagta ataccattcg ccataccacc tagtgtcaat gatgggaatt
    26641 ttctatcact tgcttcagat gaacctgagt gattattctt taaagttgtt ataccaaagt
    26701 tctttaagta atccatcatt atatctacac attcatcata agagtctcca agcatttcag
    26761 cagttttgac agctatcgtg tttgaagatt tagcaagtgc tttacgtaaa ctcatagaac
    26821 catgaccagc agtagtagtt cttggattcc atttattatt ttcttcaaac ttatatcctc
    26881 ctcgagaatc acttagagca gttgcttgtg ttatttttag agtatcaata gctggcgtat
    26941 atattgctaa aggttttata gaagaacctg gctgatgagg gttagtagca cgatttaaag
    27001 tttttctacc acttatattt cttcctccag ctagtgctct tatttcacca tttttataat
    27061 ctaagattac cattgcagct tgcggttggc taactccatc aggaccaacc atatgtcctg
    27121 ggaaattgct atttctatca aattcttctt ctaaagcatc ttgcatttta gggtctatag
    27181 ttgtgtttac aattagacca ccattgtaga ataagttttg agcttcatca tttgtatgtc
    27241 cttgttccat taaagcattt ataacttcac tttcgataag gtcttctaca gatgaagaaa
    27301 ctttatctga tgattgaggc aattttattt ctattttagc tttgattgct tcatcatatt
    27361 cactttgttt tatatatttc aattctaaca ttttctttaa aacaatttct tgtctttttt
    27421 tagcttcagg attacttaca gcctttctaa caactaatgt acctgctttt aattgtctat
    27481 aagtatcatt agatataagc tcccaagatt ttattttttc aatcatatca aagtccactt
    27541 gagtagggtc atctaaatca tcatcagttg tgttaacaaa aaataataat ttattctcta
    27601 aatcctcctt agtttcattt ccctctaact tagaagtttt ataggctgaa aatcttgatg
    27661 gatttttagt tgaaccagca agaagtgctg actctgctag agtaaggtca gctgcagact
    27721 tatcaaaata accacgtgca ccagcctcag cacctgctag acctctacca acgaagaagt
    27781 tatttaaata agtttctagt attttatctt tacttactgt tttactcatc tcatgtgcat
    27841 agtaaatatc ctttatttta cgaggtatag tttgctgttc agttgttaac agcattttgg
    27901 atacctgcat aggaatagta ctaccacctt gcttagtacc tgtaagggtc tttaatactg
    27961 acctaaataa acctttaata tcgacaccct tatgctcata aaaacgctca tcttctattg
    28021 caattacagc atgttgaaga taaggggaaa tcttatctaa tggaacgggt gtctttttat
    28081 ttacacttgg agctgtagat aatgtttttc cattagcgta tttaatagta gttgtttggt
    28141 tcgtctgttt atctaaaaca gcttctgtaa ctggagttac atctcttagt gacgcaaata
    28201 ctagaccagc agtacctgct gtaccaacaa ctagtaaaac taaaaatact atcccaaata
    28261 ctcttagttt tttaaattta tcgctttttt tagatttttt cttgtttttt gttgaacgag
    28321 tcttgttagc tgaagttcta ttaattggtt tatttgtatt accagatgag ctaacttttt
    28381 ttctcctgat tttatttcca tttttatcat tatcgttatt cataagattt taacctcctt
    28441 acttaatgca atattataac acaaatagga gtagttttgt aattaataga aaaaatgaat
    28501 ttgctataat attctgtaca ttgtatatgt ataaacgtaa tatattaaaa agtctatatt
    28561 tttattttat ttatttttga caaatattat aaaaattatg ccaaagctac atataaataa
    28621 ataggagggg ataattttga atagaatagt agatagaaaa acaaaaaatg aatttagaaa
    28681 aatcatagct atttttttag taattctttt cttgtctgta tttataggca gttttatata
    28741 tatagataaa actttaagac caacaataac agttttagca gagactaaag ctcttgaact
    28801 agcaaatagg tcgataaata aagctgttgc tgaaatggta gaaggaaaaa taaattatga
    28861 agatttaatg gatatacaac ttgataataa tggtaaaata actatgatac aagcaaacac
    28921 tataatgatg aatgaaattg catctgcaat agctttagaa atacaagatg aattaaaaaa
    28981 ggataagaca gcaagttctt acatacctat aggaacagca ttaggaagtc ctatattagc
    29041 taaatacggt cctaaacttg aagtatctat tgaacctata ggaacagtat cagtaaactt
    29101 taaaacggaa tttgagtctt caggtataaa tcaaactaga catagaatat atttagaagc
    29161 tcaaacacaa gttaaagtag taataccact tataacttca acaaaacaaa ttaaagctca
    29221 aatacctatt tgtgaaacta taatagtagg tgatgttcca gaaagttatg taaatattcc
    29281 agaaaaaaat ctaggaaatg ttttacctaa cactggaaag aataccaaca agtaatataa
    29341 agttgtatat ttttaaataa attcaaaata atagtttata aataaaattt ttggaaataa
    29401 tattaatata attttaataa aaatttatat tttatactat gtaaaatatc taatatattt
    29461 ggtctgtagg ttaaataaaa gtacctttag accaaatata ttattaataa cttaataaca
    29521 tcttaaaact tatttattta tattatttac aacatccatt taataaatac accataacct
    29581 acagctgggt cgttttcaat tgcatgtgac actgctccga ttattttatt atcttgaact
    29641 attggagtac cactcatacc ttgtacaata ccaccagttt gagctaaaag ctttttatct
    29701 gtaattttaa ttttaaaact tttactttct ggatgttttt ggttttctat atctataatt
    29761 tcaatattat attttttaca cttattaaag ttatcttgta aaattatttg agcttttcct
    29821 aatttaactt catctaaaga tgctacttta tattttttta acttggaaat atcaaagtta
    29881 ttaatttgac ctcttattcc aaaatcagta ttttctgtaa agctaccaat aatattttct
    29941 cgttttgcat ttatacatcc aacactacct ttataagatt tttctatatt aaggtgctta
    30001 gttgtagaaa ttgaacctgt tttaataggt atttttcttg cattaccaac acttattgga
    30061 tgacctacag ctccaaactc tgatgtatct ggattgatat acgttaaagt agcaaatcca
    30121 gaaagtaaat tgttaaaact aattttttct aatatgtgtt tggtagtttt cattgttata
    30181 agttgtccac ctctatttaa taaaatagat acttgttttt cttctggtaa agcatttaag
    30241 gtattggcaa agtcactata tccatcaact tcagcattat ttacctttac tatagcatca
    30301 cctaatctaa atggagatgc ttctccagga tttctaacta tgatattttt taattctgcg
    30361 tcaatttgaa gtacgtttcc aattggtatt aaatattcag gttcattttt ttgtaaattt
    30421 tgtgcataaa ttaaattatt tgagaaaaaa tataataaaa ataaaaatgt taaaacaatt
    30481 gtaaattttg acttagctaa gttggttttt ttacttaaag tattattttt aaaatgaaaa
    30541 tttttaagtt gcataaaaca cctctttctt attaaattat tttaataata gaattgaagt
    30601 tttcttgctg cttctatatt atgttgtgca agaatcatgt tttgatgctt ggaaaactag
    30661 ctcaataaca ccttagtttt cataaaaata aataaaataa atagtgtata tgataaaata
    30721 aatacatatt catataaata aaaataaaca ataactgtct agcataagaa aggagcaaaa
    30781 aatgaaaaaa tatagattgt tagttacttg tataattgcc atgggaatca taattatagg
    30841 aacatcaaat ccagttagca atttttacaa gactacaaat ttgattagtt caggaaatat
    30901 cacaaatgaa aaagcagata aagatgaaaa ttcaaacaaa aataataaag ataaaaatga
    30961 agacaaatct aattctaaaa gtaataaaga taagtcatca gaggacaaat cgtcagataa
    31021 taaaaatgaa attcaaaaaa acaaaaaact tttaatttgt atagacccag ggcatcaagg
    31081 aaaaggagat agcaatttag aaccagtagc tccaggttct tcctctaaaa aagcaagagt
    31141 atcttcagga acagagggta ttgctacaaa aaagccagaa tatgttttaa acttagaagc
    31201 atctcttgtt ttaaagagta tattagaatc taaaggttat aatgtaataa tgactagaga
    31261 gacacatgat gtaaatataa gtaattcaga aagagcaata cttgctaatg acaaaaaagc
    31321 agatatggtt gtaagaatac atgcagatag tttaaataat tcttcaaaaa ctggagcatc
    31381 tatattgatt cctgaaaaag atggaaaata tacagctcct atatatgaag aaagtaataa
    31441 atgtgctgaa tttatcaaac agaatatgga acaatcaggt atacaaatca atggaatcgt
    31501 tcaaagaggt gatttgactg gattcaactg gtcaaaagta ccagctgtat tagtagaaat
    31561 gggatttatg agtaactata atgaagacca aatgatgtca aatccagact accaaagaaa
    31621 aatgatgcag tgtatagctg atggattgga tgcgtatttt aaataagata tgaatagaga
    31681 aaaagttagg ttggtattat tttcctaatt tttatctcta ttttttattg tacataacac
    31741 aaagaagaag tttattttag gttaaattgt cgtaaatata actaaaaata ttgggaatac
    31801 ttgatttaag tttaaaaaga atatattata gtatagaatg tgtaaaattt ttttgagagg
    31861 tgttgaaatg ctagtagtag aaaatgtgag tcatggattt ggtgcgagaa caatattaga
    31921 aaatgtatcc tttagactta gaaaaggtga acatattgca ctagttggtg ctaatggaga
    31981 aggaaaatcg agttttttaa atataataac aaagaagctt atgccagatg ctggaaatat
    32041 aaaatggtct tcaagagcta cagtaggtta cttagaccaa catacggtat taagcaaggg
    32101 aaaaactata agagaagtgc ttagagaggc ttttaagcat atgtttgact tagagcaaga
    32161 aatgattgct atgtatgaca aaatgggaga agctagtgat gatgaaatga gtaagcttct
    32221 tgaagaaaca gcagagatac aaactatact tgaaaatagt ggtttttata tgatagatgc
    32281 aaagatacaa gaagttgcaa atggtcttgg tcttggagaa attggtcttg ataaagatgt
    32341 aactgactta agtggagggc aaagaacaaa agtacttctt actaaacttc tacttgaaaa
    32401 tccaactatt cttatattgg atgagcctac aaactattta gacgaagagc atataacatg
    32461 gcttactaag tatcttcaag agtatgagaa tagttttgtc ttagtttctc atgatattga
    32521 atttataaat aatacatgta atgtaatata tcatatggaa aatggagagt taaatagata
    32581 taaaggaaat tatgatgaat ttgtaagact taatgatata aagaaacgtc aagaagaaca
    32641 agcttatgat aaacaagttg aagaaagaaa aagactagag gattttgtag ctagaaataa
    32701 agctagagta gcaactagag gaatggctaa cagtagacaa aagcaacttg ataagatgga
    32761 aatattagaa agaccaaaag aaaaaataaa acctactttt gcttttaaag atgctagagc
    32821 agctagtaaa ataatttttg aaacagaaga tttagtttta gggtatgatg aagcattgac
    32881 aaaaccactt aatttccatt tagaaagagg aaaaaagatt gccttaaaag gaatgaatgg
    32941 tattggaaaa tctactctat taaaaacttt acttggaata ataaaaccat ttgaaggaaa
    33001 tgttaaatta ggtgactatt tggaagttgg atattttgaa caagagagtt caagagaaaa
    33061 tagtaataca cctatggatg aagtttggag tgaatttcca gggcttacaa actttgaagt
    33121 tagacaggct cttgcaaaat gtggacttac aaatgaacat ataacgagtc aaatgagagt
    33181 attaagtggt ggagaagctg ctaaggttag actttgcaaa gtaatgttaa aaaatataaa
    33241 tttcttagta ttagatgagc ctacaaatca tttagatgtt gaggcaaaag atgaattgaa
    33301 gaaggctatc aaagagttta agggaacagt tcttttagta tgtcatgagc cagaatttta
    33361 ttcagaaatt gttgatgatg tatggaatat agaagatttt acaactaaaa tcgtgtagtg
    33421 atttaactta ttgctggggg gatttcatga aaaagtttct aaatgtactt ttttcaattt
    33481 gtattagtat tgttataata gtaggtgtta ttaattttac tgttggattt aaacaacttt
    33541 attattttga cattgattac ttaaatatat cagagttatc tggtttatct aaagatgata
    33601 taaaattgaa ctacgattat ctaattgatt ataacttaaa taagaatgtg tctgagttca
    33661 aattaccaac attaaaatct tctccacaag ggaagataca ttttgaagaa gtcaggaata
    33721 tatttcaaaa tataaataaa ttagctaaat tattattggt agtttcttta gtaggaatta
    33781 tattgagtgt aaaaaataaa aatataaaaa tcttaaaaac tacatcaata acacttataa
    33841 ttatgccatt attattaaca gttccaattt tattaaattt tgaaaagtca tttattattt
    33901 ttcataaatt attatttaga aatgattatt ggatatttaa tcccgattta gacccagtta
    33961 taaatatatt gccagaggaa tttttttttc actctggaat gatgatttta atattaattt
    34021 tgttagtaag tatagtactg tttgtaatgt ataaattatg taaatcttta tcaataaaaa
    34081 tatttcaatg ttaaatagag ggatattttt atttccaaaa taagataata aaaaaggatt
    34141 agattaaaag ttatacaata aaaatagctg tgcaataata tttagactat gaaagtagtt
    34201 aaaaaatatt tatgcacagc tatttataat agaatttact ttttactagt gattttaact
    34261 aaatcttgaa atttactcat aatgtaattg taattatctt ttttatgagg taatatacaa
    34321 ctactgcaat ctttgatacc tttatctgta tacttaaagt ttccaccaca gttttctcct
    34381 aaagcataca aaggacaata gcaaaataga cagttaaact cttctggttt attagtttta
    34441 tgacaaggga aaaattcgca atctttgtga ttaaaaaact tataattttc gcccatattt
    34501 tcctcctaat taaataaaat ttaaattttt tatactatat aagattttgg tgataatata
    34561 attatactaa ataattttat gaatagtcga taatatagta ttattaatca tagtattaat
    34621 ggtataatta gtcaacagaa aaataatgta aaaataatgt aagtgtattt tgtaattaga
    34681 atatgtttta taacaaaaaa tgaggtgatt aagtgaaaga aattcaagaa aaagcacttc
    34741 ttgtaggtct gaatttgact acaatagtga aaaaaaatga tgatatagac actaatgaat
    34801 ctatggaaga attaaaagaa ctaacaaaag ctgctggggc agaagttgta ggtagcttaa
    34861 ttcaaaataa acactctgta gatgcagctt attatatagg aaaaggtaaa gtagaagaaa
    34921 taagagccta tagtgactct ttagatgcta ctttagtaat ttttaatgat gaattatctg
    34981 gtgctcaaat cagaaatata gagaatgttg ttggaagaaa agtaatagat agaactactt
    35041 taattttaga tatatttgct caaagggcac ttagcaaaga aggtaaatta caagttgaat
    35101 tagcacaatt aaaatataga ttgccacgac tttatggaat gggtggtgag atgagtagaa
    35161 caggtgcagg gattggtact agaggtcctg gagaacaaaa actagaaata gataagagac
    35221 atatatttaa taaagcagca gatataagac gtgaacttaa agaagtaaaa aagaatagag
    35281 agacacagag agttaagcgt ttaaagtcaa atataccgat tgtagcactt gttgggtata
    35341 caaatgcagg aaaatctacc ctgttgaacg aattaataaa gacacacaag gattatgaac
    35401 aagaaaaaga agtttttgta aaagatatgc tttttgcaac tttagatgta accttgagaa
    35461 aggctctttt gccgaataaa aaagaatttt tagtagtaga tactgttgga tttgtaagta
    35521 aacttcccca tgatttagta gaagcgttta aggcaacatt ggaagaagta cagtatgcag
    35581 atttaatctt acatgtaatt gatgcaacaa atactagtta tgaattgcaa aaaagtacga
    35641 ctgaaggtgt attaaaagaa cttggtgtaa atgataaaaa acatatactt gtatataata
    35701 aagtggacaa gctagaattg gatatatacc ctaaaagtca agaagatatt gtgtatattt
    35761 ctgcaaaaca aggcataaat atggataagt tattaaatat gatagaaatt gctttaatgg
    35821 aaaatactta ttcagtaagt ttgatgttac cttatgagag aggtgatata tttagtagaa
    35881 taaaagataa atataatgtg gagaattttg agtatgggga aagtggtata acgctagatg
    35941 tagatttaga tgaagaagat tttaatatct atagagagta catactagaa aaataggttg
    36001 aatatggata taaactggga aaatatagat aatcttgaag attattttat aacctatctt
    36061 ctttataaag aatctaaaac agtatctcaa attagtaaaa ttagaaatat ttcaagtact
    36121 gaagcaagtg agcaacttat acaagcaaag ttaaaaataa aggaaatgca aaaagatgat
    36181 tttgaggcat ctaaggatat tttggataaa tttctagaac tagataaaat taagagactt
    36241 gattttatgg atagcttaga tgacgaaaaa atggtatact ttaagagaaa ggttttcaaa
    36301 agaatccttg tagaaaaaaa tgctgaagat ttaattgttt taatttgggc gacaggtgag
    36361 ctaaaagatg ataggttttt gaaattgctt catcagctca caaaccacag acactcagat
    36421 attagaagaa taacttattc agcaatcaga aagattgaat caccaagtag tagagaagta
    36481 ctgcaaaaag gtctctacga caaaaatgca cagactaggc agtactgtgc aaaagctctt
    36541 tcaaaattgg gagatgaaaa cagtttaaag attttacagc agttgaaaga caagaataag
    36601 aattttgaaa aagaatatgt gcttagagct tatgacgaag ccattaagtc attggaaaat
    36661 gcaaaaaatt tgaaaaagta attttttatt aaagcaattt gtttgcttaa tgatgtattt
    36721 ctaaagttta acaaggtaat aaataaatta atgggagggg tttcaaatga tagtaagacg
    36781 ttgtgcaggt ggagtagtgt tttatgctaa taaagtactt attgtaaaaa atgatagggg
    36841 tgaatggaca cttccaaaag gaaagatact aggaggggga cttccatatg agagtgctgt
    36901 tcaaagagtt aaagtagaaa ctggcataga tgctaaaatg atagatgtgg ctggcgatac
    36961 tatgtatgaa tttttctcaa gaagtagaca acaagaagtt tgtaatgcaa taatgtggta
    37021 tgttatggaa gcttgtaaca ctgattgtgt acttgctcct gaatttcaag aaggtggatt
    37081 ttataaagta aaggatgctt tagaaatgtt atcacatcat aaagagcaag ctttagtaga
    37141 agtatcttat aaaaagttta aggaattaaa gaaattagtt tcagagggcg atgatgttcc
    37201 agcaaattaa ttgtgaattt agaggattct gaggaaggga tagccccttc cttttttaat
    37261 tgaagcagtt ttaagcgtaa attatatagt ctctttaaac tatgatttat caatttagag
    37321 atttagtagt ttatatttaa aatcagtaag atatatttaa aataaaaatt aaaaagtata
    37381 caattttatt atataattgt cttatgatgt tgattaaaga attattaaaa gtccatttat
    37441 ttttaaatag aataatttta taaatatata tttgatgtgt atattagaaa atgattaaaa
    37501 taggaggaaa tacaatgagc aattacagga cattacatga atttggaacg gatgaaataa
    37561 cgattgaaaa gtctgttttt attggatatg ctaagcctat aaagagtgaa gaagaagcat
    37621 tagagtttat aaatgaaata aagaaaaagc ataaagatgc aactcacaat gtgtgggctt
    37681 atactgtagg taaaaatatg aatatacaaa gatatagtga tgatggagaa ccacaaggaa
    37741 cagctgggat acctacatta gaggttataa aaaaagaaga tttgagagat gtagcggttg
    37801 ttgttactag gtattttgga ggaacaaaat taggtgcagg tggtctagtt agagcttata
    37861 caaaaggtgc taaactggga cttgaagcag gtaagataat ttataaggta atgtatcaag
    37921 aagttaaggt taagatagat tatactcaac ttggaaaagt gcaaaatgag ttaatgaatt
    37981 tggggtattt tataaaagat actgtatatg aggataatgt tgaaatagta gtatattcaa
    38041 gattagaaga tgtagagaaa ttatcagaaa aaatgattga tataacaagt ggaacaggaa
    38101 aaatagtttt aggagaggaa ttttatttgt ctgaacaaaa tggagaaatt ttaatataat
    38161 ctaaaaggtt attagaatag ttaatacctc aaatagccaa ttagtatttt taagtgaaaa
    38221 gccattaaat tgatttaatc taaatcaatt taatggcttt ttatcggatt ttgcacagaa
    38281 tccttttttt acttcaaata aaccgactaa aagtaaaaat atcccaaata ttttttttaa
    38341 tatatctgaa gacagataat tagctaatag tgaaccaaat acagctccta aaaccccaaa
    38401 cacagctata ggtataacca atttaaaaat tacattttta ttttttatat gtatagcaag
    38461 tgctataagt gtcataggga tagatgaaag taaatttacg ctttgtgcta ttttagtgtc
    38521 tatgccagca agtaaaacga gtgcaggtat aaggattgtt cctccaccca ttcccattcc
    38581 tccaataatt cctgcaaaaa atccaattag agcaaataac attaaaacac catccttaat
    38641 gctgcaatta tcataattat tccaaaagaa attctaagaa actttccagt aaccttattt
    38701 agtaatttag ctcctataat accaccaaga atacttccaa ctgcaacttt aattgttaag
    38761 ttaacatcat aagtaccttt agatacatat aaaacagaac ttgctgtcgt taagaatatt
    38821 attatagaaa gagcagttgc atgagattta tgttcttcta ccttaacgat gtcatttaat
    38881 attggaacta aaagagtacc tcctccagaa ccaaaaactc catttataaa tcctgtaaaa
    38941 actccaatta tcgtattttt taagttaaaa tcaaagtatt tttgctttaa atcatcagtt
    39001 tttttcataa aaatcacctt ctgaaatagt tttaccaaaa taggcaaatt atatttatat
    39061 aaaataagat taaatataat ctttcatgtt tgtttaattt tgcataaaaa aaaacatatt
    39121 aaaacaacat attaatatat ggtataatat attaaaacaa agtagcaaat ttgagaggag
    39181 ataacaatgt caaatataaa aatacaaata gacaaaactg tagaatggct tataaataag
    39241 gttaatgagg caaatgcaaa aggattaatt gttggtgtat ctggaggaat tgattcggca
    39301 gtagttgcta atttaattaa aaaggcattt ccagaaaact ctatgggagt tataatgagc
    39361 ataaaaagta atcctcaaga tagagaagat gcacttaagg ttatagaggg atgtgatata
    39421 gaataccttg atttagacct tatagaacca caaagtgcta tattagacat ggtagtagga
    39481 aatttaaaag ataaacattt atatagagaa gaatatttaa agatgacaga tgcaaactta
    39541 agagcaagag taagaatgag tacaatttat acaattgcta acaatctagg atatctggtt
    39601 gttggtacag acaatgctgc agaaatacac acaggatatt ttactaaatt tggtgatggt
    39661 ggagttgata ttttacctat tgcaaattta actaaaggag aggtgtatga atgggcaaaa
    39721 gagcttggtg ttcatgaaga tttaataaac aaagctcctt cagcaggtct ttgggaaggt
    39781 caaacagatg aagatgaaat gggaactact tacaatatga tagatgctgt attagaaggt
    39841 agattggatg aagttcctaa aagagaccaa gagataattg aaagacttca tagattaagt
    39901 gaacacaaga gaaaaacgcc tgcacaacct cctaagttct aatttactat ttataagatt
    39961 tgtgataaaa tttacaagtg atgaaaatta gaataattaa ttgtacgtat taaggagtgg
    40021 aaatatggga cgtataggta atataattaa tagaaaaggt aaacaagatg ctcaaagagc
    40081 aaagatattt actaaacatg ctagagcaat agcagttgca gctaaagaag gtggagcaga
    40141 cccagagtac aatgcagcgt taaaaacagc tatagaaaaa gctaaggcag ataatatgcc
    40201 aaatgataat atagatagag ctatcgctaa aggtgctgga gcaggtgctg gagaagacta
    40261 tgaaactata gtttatgaag gatatggacc tggtggagtt gctgttatag tagaaacttt
    40321 aactgacaac aaaaacagaa ctgctggtaa cgtaagatac tattttgaca aaaacggagg
    40381 aaacttagga actagtggat gtgtgtcttt tatgtttgat aaaaaaggac aaatattagt
    40441 tggtttagga gatggagttt cagaagaaga attgatggat gttgcattag aagcaggagc
    40501 tgaagatttc ataacagaag aagatggtta tgaaataata actacaccag aagatttttc
    40561 tagtgttcgt gatgagttaa aagctaaagg atatgagttt atatctgctg atgtgaagat
    40621 gatacctcaa actacaactg tattgactga agaatctcat ttaaaaatga tgaataaatt
    40681 agttgatatg cttgaagaag atgatgatgt tcaagatata tatcataact gggaagtaga
    40741 ataaatagta taagcatatt gaaatttata agtatttaat caattttaac atgtgataaa
    40801 ttttatagaa taagaaacac taaaatagac atacgcacct ctaacctata tttgcataat
    40861 aatatgtaag cataggtaag gggtgttttt atatttatag gttaagaaat ttaaagtaaa
    40921 ttttgagctg gactcgattg tctataaata attgatgggt atataacttg ttgctgaata
    40981 ccatttatac gcccagttac tgtatcaata atcatcctaa gactacgttc tgctgtctct
    41041 tccattttta aatctataac tgtcaaagga ggaatacttc ttgccattag tcgtgaaggt
    41101 ccattacttg ctgcaaatat ttgaatatcc tctccaatta caattcccga ttcatgaaat
    41161 gcattcatca taccaagagc taccacatca tgattacaaa aaattacttt ttttagtgaa
    41221 ttttttgcaa gcattttttg agcaatatca tatccactat ctattttatt agaacaataa
    41281 tatatagaat ctgatagatg tattccatgt tctttacatg tttttaaaaa agatttaccc
    41341 cttaaagtca tacatgtaaa ggaagttggg ttgaatacaa cagcaatatc atttttacca
    41401 ttttgaatag catgtaaagc tgctatacga ccagtttcct cattatcaat agatacactt
    41461 gaaaaaccag gtatttcacg atttacaaca actacaggaa ttttaggttt attttttact
    41521 agatattcta tatcatcagc attgatacct gttaaaacaa ttccatcata ataattattt
    41581 gcaaaaaagt attcatcatc acatagatga ccaactttgt aaggtcgtat gatgatattt
    41641 atgtttaaat gttctaaaga aattatgtgg tttattgctt gaagtatatt ggttgatgcc
    41701 aactcaaatc cctcatcctg ccaataaaaa gctattttaa tattattatg tttaatagga
    41761 gtagagcgtt tatatccaag cttatcagct gaatttaata taagctcttg tgtatgtagc
    41821 ggaatcctgc gttcatttcc tttaccactt aagacgtacg agacagttgc tgaagaaaga
    41881 ccacagtctt ttgcaatatc tttgattgtt gccatgataa aatcctcctt aaatagttgc
    41941 ttaaatttgc ttataccaat aatcttaaca tatattcaac taaagaacaa tattctacaa
    42001 ggtgcatatt ttacaggatt agaataaata aattaaaaaa aaacttctta cttatgttga
    42061 cataatttag tttataggat tataatatgc ttaaataagc ttatgattaa cttaaattaa
    42121 gcaaagttaa gcaggaacat gcaatgctag cttgcaatgt gactgtaaag ggggaaaagg
    42181 tatgagtaag gtaaatatat ttgaagttgg tcctagagat ggatttcaaa acctgaaaga
    42241 gtttttagaa actgataaaa agcttaagat tattgatggt cttattgctt cagggattaa
    42301 acaacttgaa attagttcat ttgtaagccc aaaagcaata cctcagatga aagatgcaaa
    42361 agaagtagca acttattgtg tagaaaaata tcctgatgtg aaactttatg cattagttcc
    42421 aaatttatac ggtgctaaag ttgcatggga gtgtggtata agagatatat cctatgttat
    42481 atctttgagt gaaactcata ataaaaaaaa tataaatcgt acccatgaag agtctattga
    42541 tgagcttaaa aaaataatga atacttatcc agacatgaat atatgtattg gaatggctac
    42601 tgtatttgga tgtccttttg aaggtttacc aaaaattgaa gatgccataa gatttgctaa
    42661 acgcctttgg gaaaacggta tcagggagat aaatctagca gatacaatag ggattgctga
    42721 ccctaagcaa gtaagagaaa ctattagtgc attagttgaa gctttaccag agtgcagatg
    42781 gcaagtacat attcatgata ctagaaatat gggaatggtt aatactcttg tggctattga
    42841 atctggaatt actactgttc agtctacact tggaggatta ggaggatgcc catttgctcc
    42901 tggagcatct ggcaatactg caactgaaga tttagtatat atgcttgaac gtatggggta
    42961 tgatacagga gttgattttg ttaaacttct tgaattatca aagtatcaga agagttttgt
    43021 aaatggagta tatagtggtc atcatataaa tataaagtct gatagttgta attgttagtt
    43081 ctattgatta atcgattaaa aaaatatgtt gattaaaaaa atatgctgat tggtaaaata
    43141 gatttattga taaaaaatat aaattataaa atacattatc taataaaaca aataaaaaaa
    43201 ttgaggggga ttagtatgga tattatagct ttacttttat ttttaggagt tattattatt
    43261 gcatttgtac gtaagaataa tgcaggtatt ttggctctag ctattggtgt catagctgtt
    43321 agaatttttg gaatggatga taaagaactt tatggagcta taagcaactc aatgtttact
    43381 acacttgttg gtataacact actctttgca atagtgaact caacaggagc attaaagctt
    43441 gttgcagaaa aaattgtagc tgcttctggt aaacgtgttt ggcttatacc tatagcagtt
    43501 tttatagcag gatttgttgt tgctggtgtt ggacctgggg caattccagc acttgcaatt
    43561 atcccagcaa tggctgttcc tgtagccata acagttggtt ataatcctgt aatgcttgca
    43621 cttataggtg aatgtggatt aatggcaggt cgtatgactc ctattactcc agaaggtcaa
    43681 attataacaa ccgttgcaga aggggttgga attgaaaatg ttatgcctac tatcttagcc
    43741 tgtcaaactc tttctacaat tgtattttca atagtattgt ttataatttt caaaggatat
    43801 aaacttaaaa aacctataaa tgttatagat agtaagcaaa tagaaaaatt caatataaag
    43861 cagataattt ctctaataag catagtagtt atgattgctt ttatcataat ttttgatatg
    43921 aatgttgggc ttgcagcttt tgcagtttca gctgtactta tttttttcaa tattgctgat
    43981 gaaggagaat gtatcaaatc aattccatgg agcacgatag taatggttct tggagtaggt
    44041 gcaatgatga caatcgtaga tgcagcagga ggtattgact tgatgagtaa tgcattatca
    44101 tcactgatga atagtaaaac agctactcca attatgagta tttcagcagg acttatgtct
    44161 atggttagtt ctgctttagc agtagtttat ccaactatga tgcctatgtg tgttgatata
    44221 gctaaggcag ttggaggagt agacccacta gcacttattg ctgcagttgg tgttggaggt
    44281 tctcttgctg gtgtaagccc actttctact ggtggggcat tgattttagc agctatgggt
    44341 tcaagtaaga aagactttaa taaggaagaa caaaataagg tttttgtaca gcttttcatt
    44401 atgagtgcag ttggcttatt ggttattgca gtagtatcaa tattatcatt taatgcaata
    44461 acacatttaa tgaactaatt agaataactg aaaataaatc attaaattta acttttaata
    44521 ttaatcaata taatataatt tggaggggaa gttatgaaaa aaggagcatt aagcaatata
    44581 aaggtacttg accttacaag agttcttgca ggtccatatt gtacaatgat gttggcggat
    44641 tttggagctg atgttataaa aatagagatg ccaagaaaag gtgatgatac aagaggtttt
    44701 ggaccttttg taaatggaga gagtttatac tatgcaaata taaatagaaa taaaaaaggt
    44761 atcactctaa accttaaatc agaagaagga aaaaatatat ttttagaaat ggtaaaacat
    44821 gctgatgttg tagtagaaaa ttacagacca ggtgtaatgg aaaagttagg tctgggttat
    44881 gatgagttgg ctaaagttaa caatcaaata atttatggag ctgtatcagg atttggatgt
    44941 tatggaccat attcagaacg tccaggttat gatattattg cacaggcaat gggtggtctt
    45001 atgagtataa cagggcaaga aggaggagaa ccaacgagag ttggtaatgc tatgggtgat
    45061 gtactaggtg gaatgaattt aactattggt atactcacag cactcaacgc tagaaaaata
    45121 ataggaaaag gacaaagagt agatgtttcc ctcgttgatt ctgttgtatc gtctctcgaa
    45181 actggtatac agagatactt tgattcagga caacctgaac ttatgggtaa tagatatgct
    45241 tcagcctatc cttatgactc ttttaaggca agtgatggaa gttttgtaat aggctgtggt
    45301 aatgacaaac tttttgaaaa gttatgtaaa aaagttcttt ttagagaaga ccttttaata
    45361 gatgagagat ttaaaacaaa tcttaatcgt tgtgaacatt acaaggctct taaaccagaa
    45421 atagaaaaat ggtctgttaa gcatacaata aaagaagcag ttgacttaat atcagaggct
    45481 ggagttcctg ctgctcctat ctataatctt gattgtgtta caacagatga acatatagca
    45541 aaagcaagag aaatgattgt agatattagt catcctatta taggaaatat gaaagtcaat
    45601 ggaaatccag ttaagttgat gacaacaaaa gctaaaattg atatgccagc accatctcta
    45661 ggggaaagta acgaggagat ttataatggt tggcttggtt ttgatagtga taaaataaaa
    45721 gatttaaaag aaaatggagt tatttgatta aaaaatagct aaacacactt gtgactttag
    45781 tcatgggagg ttcagttaga ataatatcaa aaaatattaa atcaatactg taaatcaaat
    45841 atagaattgt cagaagcatt taagatggga aaagctactt agacattgag tctttgtggc
    45901 ttttcctatt ttcattaaga attttatatt atcagaatag atatgtacta aactaaagta
    45961 ttaacaattt aaattaatag aaaataaact acctaattat gttgaataaa gactacttta
    46021 gtgaaaataa acttatagag atgctattta aattatttta atggaaatat caaaaatgag
    46081 actgcattct caaaaatgag actgcgtttt ttaagtattt tctcgaaaat gagacaaaaa
    46141 tattgtttta tgggttttat tgaagaaaaa ttcttctgtg aattacaatt taatcaattt
    46201 taaagttgaa aattattaaa aatttagtga aatttatgta tatttcgttt attttattaa
    46261 aaataacaat tttttactaa aaataaagaa cttcaatggt gattggcata gtacttgcta
    46321 ttttatatta ataagaaatg tagaaaaaat aacaatgtat aatgggggga atatgatgga
    46381 atctgtaatg gaaaatttga acaatcttaa ggtggagctt aaggacaaag tctgtgttat
    46441 tactataaat agacctaaag ctttaaatgc tcttaatagt gacactctta gggaactatc
    46501 acaagttatt gatgtagtat cagaaaatga ggcaatatta ggagttatta ttactggaga
    46561 aggtaaagtt tttgtagctg gtgctgatat acgccagatg caaaactata aaagtgagga
    46621 aggcagaaaa tatgcaggtt atgctcaggg aatttttgat aaaattgaag ctctggagaa
    46681 aacagtaata gcagcagtta atggatatgc attaggtggt ggatgtgaac ttgctatgag
    46741 ttgtgatatt agaattgcat cagaaaaggc tatatttgga caacctgaag ttaatttagg
    46801 agttattcca tgttttggtg gaactcaaag attatcaaga cttgttggaa ctggaatagc
    46861 taaagagtta atttttacgg ggagacaagt aaatgcagaa gaagctaaat ctattgggct
    46921 tattaataaa gtggttccaa gtgatttact tttagaagag tctatgaaga tgatgaacca
    46981 aatagttgaa aaagcgccaa tagcaataag atatgcaaaa gttgttatta acaaagggat
    47041 agatatggat ttaaagaatg ctcttgaact agagaaagat attgcaggtt taacttttgc
    47101 tacaagagat aagcaagaag gaatgaatgc atttatagaa aagagaaagc cagtttttga
    47161 aaataagtag ctattataac taaaattgga acaatgtaga atatattcaa aatctaaatt
    47221 tcgtgaaaaa atacatatag tttaatttta aaaggagtta atttatgaat acgggagtaa
    47281 ttataagttt agtagggatt atcatagctc tatctcttct agttgtaatg gttatgaagg
    47341 gaataaatat ttttattata gcaattgtat gttcatcatt agttgctata acaggaagcc
    47401 taaatgtgta tgatgcactt aaagtggatt atatgacagg gtttgttgga tttcttcagg
    47461 caaatttctt tattttccta acaggtacat taatgggaaa aataatggag attactggtg
    47521 gagcaaaatc tattgctaag atgattgtaa gatggattgg gaaggataaa gcactacttt
    47581 ctattccaat agcttgtgga atattagcct atggtggagt aagtgttttc gttgtaagtt
    47641 ttgcagtgtt tccgatagct ttagaaattt ttaaagaagc tgatttgcca cgaagattta
    47701 taccagcagc tttgactttt ggatgctcaa cttttgctat ggttgcacca ggggcaccac
    47761 aaattcagaa tattgttcca gcatcaacat tagggacaga tattatggct ggagcagtaa
    47821 atggttttat atcatgtgca gtaatgttta tagttggaag tacaattttg tacaaaatgg
    47881 tttcaaaaga aaaagccaat ggtggtcatt ttatagcaca tgagtcagat atatttgatg
    47941 aagcagaagc aactaattct aagagagaaa atggtcccaa tggtgtaatt gccttaattc
    48001 cattgatagt gtcaatttta ataattaatg taaaagttaa tggaaaagcg atagtaccaa
    48061 ttgaggtggg agtatttatt ggagctttat tagtatattt attgttaaat aaatatcaag
    48121 ataatactaa aatagttgga catgttggtg atgcatgtaa aactacagta gtagcaatat
    48181 gtaatacttg tgctgtagtt gcatttggtt cagtagtaaa atcagcagtt ggttttgact
    48241 tcatagttaa tgccatgact aatattcctg gtcctcctat tgcaggggct gcagttggaa
    48301 ctacagttat tgcaggtatt tgtgggtcag cttcaggtgg actaggaatt gcagctccac
    48361 ttcttggacc agtattttta gccaagggtg tttccgctgc agcattaact aggactatgg
    48421 caatttcatc agcagcatta gattcacttc cacacaatgg atatattgtt actgtaacaa
    48481 atggtctttg taaagaaaca cataaggatg cctatatgcc aatattctgg gtaactgttt
    48541 taaccccatt tattggaacc atagtagcag tcctactgtt tacaatgttc ccgggattgc
    48601 cataagagaa aagataaaag gtataaatta tattttgata ttataaaatc aattttcaaa
    48661 atatatacaa taaatttcat gttttaatac tgtaaatatg tatttaaaat aacaaattga
    48721 ccatataagg aggaattgaa agtgttaaat aaaccattag agggagttaa ggttattgac
    48781 ctaacgtatt ttgtagcagg tccagggact tctaaaatat tagctgactg gggtgctgac
    48841 gttattaagg tagagccaag ctttggagac ccaggaagaa agacaggggc aacaatgaca
    48901 atgccaatag atgattataa taatcctttt tacagcacat ataattcaaa taaaagaggt
    48961 ttgtctataa atcttaagag tgaaactgga attgaaatta tggataagct tttaagcgaa
    49021 gcaaacgtat ttgtttcaag ttatagaaca ggagcgttaa aaagactagg tcttgattat
    49081 gaatcattaa gtaaaaagca tccacacctt atctggggtc agataaatgg ttttggcgat
    49141 tttggacctg ctaaggataa tgcaggattt gatacagttg ccttttgggc aagaagtggg
    49201 gctatgcttg atatagcaga aaaagataca agtccaataa atccacctat agcctttgga
    49261 gatgcaacaa cttcttgtag tttatctgga ggaatatgtg cagctttgta ccagcaagct
    49321 aagactggca aaggacaaaa agtaatggca tctttatttg ggcaagcaat atggaatgca
    49381 tcatctttat tagcatctac acagtttagt gatgagtatc caaaaactag aaaaaatgct
    49441 atttcaccag taataaattc ttatcgttgt aaggatggaa agtggatatt cttaagtatt
    49501 ttagagcatg aaagatattt tgaggcactt tgtagattat ttggaagaga agacttaata
    49561 aaagaagaga aatttgcatc ttcagtagaa agtaagaaaa atgccacaga acttattagt
    49621 attttggatg gtgaatttgc aaagtttact caaaatgaga tggttgaaaa acttactgct
    49681 gctgatatag ctcatgaaaa aattcaacat gttaatgatg tagtgactga tgaacaggct
    49741 attgctaata actatatata tgaacataca agtaaaaatg ggaataagac aatgcttgca
    49801 tctactccag ttaaatttgg aaatattgaa gttaacatga cttgtgatgc tccacttata
    49861 ggagaacatt cagatgagat attgagagaa cttggataca gtgattctga tatagaagct
    49921 ctaattgaat ctggaattgt aacaatgaat aaagaagcat gtgaaatatg ttaataaaca
    49981 tgtgaagtat gttaataaat aggagggaat tttatggatt ttagattaac agaagcacaa
    50041 ttaatgcttc aaagagtagc aaaagaattt gcagagaatg aaatagcacc aatagctgct
    50101 gaaacagaca aaacaggtat ttttccaagg gagcttttta gcaaaatggc taaaatagga
    50161 tttaatggaa ttggaacacc tgtagagtat agtggttctg gtggggcaga tatagaaaag
    50221 gtaattgttg ttactgagat tgccaaaaaa tgtgcagcat ctgcagcaat attatcaata
    50281 cacacaatat atgctcaagc tattttgaaa tttggtactg aggaacaaaa gaaaaaatac
    50341 cttccaatga tggctgaagg tgggtgtgta ggtgcttttg ccttaaccga acctaatgct
    50401 ggttcagatg ctgcgagagc tgcaacaact gcaattattg atgaagaaac tgatgaatat
    50461 gttttaaatg gaactaagtg ttttatttct ggtggaggtc aagcagaatc attaataata
    50521 tttgcattga cagacccatc aaagggaata aagggaatgt ctgctatcat tgtcgacaaa
    50581 ggtactccag gtttttcaat aggaaaaatt gaagaaaaaa tgggtattca tggttcagaa
    50641 actgcagagt taatatttga taactgtaga gttccaaagt ctaacttatt aggaaaagaa
    50701 ggtaaaggat ttaatatagc tatgacttgt ttagatggag caagaattgg agttggagct
    50761 caagctgtag gaattgctga aggggcttta gaagaaagta tcaaatattc taaagaaaga
    50821 gtccagtttg gcaagccaat ttcagctttg caaggtattc aatggtatat agcagatatg
    50881 gcgacaatgg tggaatcagc aaaattatta gtatattatg ctgcagattt gaaagctaga
    50941 ggagaaaaac acactaaaga agctgctatg gcaaaataca atgcgtcacg tactgcaaga
    51001 gaggttacaa atttagccct tcaaatccat ggtggatatg gatatatgaa ggattatcca
    51061 ttagagagaa tgtacagaga tgccaaaata acagaaattt acgaaggtac ttcagaaata
    51121 cacaaagttg ttatttcaag agcagtacta ggataggagg aaaagaatga atatagttgt
    51181 ttgcttaaag caagttccag atactaatga agttaaaata aataaagaga caggtactct
    51241 aataagagat ggtgtaccaa gtataatcaa tccagatgat agaaatgctt tagaagaagc
    51301 tctaaaaatg aaagatgaac taggagcagt gattaaggtt ataagcatgg gacctcctca
    51361 agctaaaagt gctttaaaag aagctttggc aatgggagct gatgaagctt atttaatctc
    51421 agatagagct tttggtggtt cagatacttg ggctacatct acaattattg ctgctgctat
    51481 agagaaggtt ggaaagtatg atgtaatatt ctgcggaaga caagctatag atggagatac
    51541 agctcaggtt ggaccagagg ttgcagaatt tttagggata ccacaagtta cttatgcgaa
    51601 agaagtaaag gttcaagatg acaaattact agtaactcgt tatacagaga caggggatta
    51661 tttaatagaa gcaaagatgc cagttttatt aactgctata aaagagttaa acaatccaag
    51721 atatccaagt gtcaagggaa tcttagaagc ttataataat ggagatgcaa agattactgt
    51781 attaacactt gcagatttag atgttgatac aactcaaatt ggattaaagg gttcacctac
    51841 aaacgtttat aagtcttttg taccagttaa agacaagcat aatgaaataa tagaaggtat
    51901 aaataaaaaa gaaaaagctg agaaactaat agaaatacta ttcgatttga aactagtata
    51961 ggagtgttaa gatgatgaga gcaaaagtta atcaaggtat aaatttaaat gattataatg
    52021 gtgtttgggt aataggagag caaagggaag gtaaaattaa tcctgttacg atagaactta
    52081 ttggtgaagg aagaaagctt gctgaccaat taggaaaaga gcttgcagtt gtgatagctg
    52141 gatatgaagt agagaaagaa gtgaaggaat tacttcacta tagtgttgat aaagtatatt
    52201 acatcaatga ccctcttctt aaagatttca ctactgatgg atatgcaatt tcaattgcaa
    52261 atttaatcga aagaaaaaag ccagaggttg tattggttgg tgctacttct ataggaagag
    52321 atatagcgcc tagaattgca ggtaaggttg gaacaggtct aactgctgac tgtacaaaac
    52381 ttgaaataga ttcaacagat aataagttat tacaaacaag accagcattt ggtggaaatt
    52441 taatggccac tattgtttgt ccaaaaaata gaccacagat gtcaacagtt cgtccaggag
    52501 ttatggcaaa agctgtaaga aacgaatcag agactggaat tttagaagtt gttactccag
    52561 aacttactga aaaaatgatt agaactagat tagtagagat acttccacaa gaaaagaaat
    52621 ctgtaaatct aactgatgct agaataatag tatcaggggg aagaggatta aaaagagcag
    52681 aaggatttga gcttataaaa gaattggctg ataaattggg ggcagagatt ggtgcttcaa
    52741 gagcagcagt agattcaggg tggatagaac attctcatca agtgggacaa acaggaacta
    52801 ctgttagacc agaactatac attgcatgtg gaatatctgg agcaatacaa catttagcag
    52861 gtatgagtga ttctaaatat atagttgcta taaataaaga tgcaaaagcc cctattttta
    52921 gtatatgtga ctatggaata gtaggagatt tatatgaaat tatacctgaa atgatagaat
    52981 cattaaatag gtaactttta gaaaacattg aatatgtaga atgataagtc ttataatatt
    53041 gtattgtaag acttattatt tttgctgaag gttatagtgg ggagatgaaa caatgaaaga
    53101 gcagtggtat aaggatatat ttgcaagagt attatctatg actgatgatg gttttattgt
    53161 tgtaaataca agtggagtaa taatagatat taatgataaa tattgtgact ttttaggaaa
    53221 agaaagaaaa gatataatag gacaaaatat acaaagtatt ataccaaata caaaaatgct
    53281 agatgttatg aaaaataagt attgtgaaga aggagctata catcattatt caggtggaaa
    53341 tacaaaagaa aaaagtgtaa tagtaagtag gtcttatgtg gaaaatgaca atggagaagt
    53401 agttgcagga gtagctcaag ttaaatttag attgcaatca tttgatgtag ctaaaaagtt
    53461 aatgtcagaa tatatggagc tccaatatta taaagagcag tttaaggata actgtggttt
    53521 tgacaagctc ataggtgaaa atagagactt catagaactt aagaaaacag gtgtgaaagc
    53581 ttctaaaaca aactttccag tacttttaac gggtgaaaca ggcacaggta aagaagtttt
    53641 tgctagagca attcacaata atagcagtag gtcagataaa cctatggtta gtataaattg
    53701 cgctgcaata ccagaagaat tattagagtc agagctattt ggctatgacg aaggagcttt
    53761 tacaggagca aaaaagggtg gtaaaaaagg taaattttta gttgcgaata atggtacaat
    53821 ctttttagac gagataggtg acatgccttt gaccatgcaa gcaaaacttt taagagttct
    53881 tcaggaaagt gagatagaac cagtgggagg tcttaaaact ataaagatag atgtaagagt
    53941 aatctctgcc acaagaaaga atttaagtaa aatggttgaa gagggattat ttagagaaga
    54001 cctatactat agattaaatg taataaacat tcatatgatg gaattgaagg acaggcaaga
    54061 tgatatttta ttgctagcaa attatatatt aaacaaatta aatgttgaat acaaggaatt
    54121 gaaggtttta agtgataagg ttaaaaattg ttttattaac tatacttggc cgggaaatat
    54181 aagagaacta caaaatgtaa taaaaagtgc ctatgctgta agtgatgata tggtcattat
    54241 gatgtgtgac cttccatcta agatggataa tatcagcaga gtagctcaat gtaatgtgga
    54301 tagcaattgt tcaattcatg aaatggttga gaattatgaa aagagcttaa ttattgatgt
    54361 gttaaggaag tataattgga aatgttctaa ggcagcagag gttatgggaa tacacaagag
    54421 tcttttatat aagaaaataa aaaagtatga aattgaatta aataattaag ataacttgaa
    54481 ttaaggcaat gctaaaaaag cgcccttatt caaagcgaac tgtataaact gttttatgca
    54541 gttaaaacgt gtaaaagtag atgtgaagta ctaatgtcaa tcattagtac ttttttatta
    54601 tatttataat tactatctat atttcagtat cttttgatat ctaagattat tatgtttaat
    54661 ttagtatata acaaaaccct cttagttatg gacagttttt tatattttaa ctgtctacta
    54721 agaggggata gtttagttta aatttagagt aaattttatc tagcatttat aaaaaatatt
    54781 ttgaatagct tttttgttat ttagtcataa ttttctactt ctttatcaag ttctttgcca
    54841 caagaatcac acttatatat agcattgtat ccgtaatcat attcatcaac tattttcttt
    54901 tgtctaaata caccaccaca ctcacatagg aaaccaattt ttataagtgt aacgataaat
    54961 tgtgatttta taaattttat atcattgcat ttatcgttag tgtaaaatgt ataaatataa
    55021 gagttatcgc actcatctct taattcaaat ctttcaatat atgtgttatt ttttaattcc
    55081 tcaaattctt ctttagttaa atatgatttt tgttgcaatt catgtaaacg cttaatttgg
    55141 tccattattt atatcctccc aataatttta gtattttaag cttagataag gaatctaagt
    55201 tttattaaat ttatcccaag gaattattta aatcatattt agttttatta attacaacat
    55261 atataaatta cacgaaaata aaactaattg taaaaataat atcctgtgaa aacctaaact
    55321 actaacccct tactaacccg aaaactaaaa aaagaaaata tatccaaata taagtgatat
    55381 ataaataaag atgtttcata taaatttaac tttaaatatt ctgacaataa tatatataaa
    55441 tatattatag ttaaaatata ccacatattt tgggtattgt atactggaat tagagtaatt
    55501 aatttatttt ttaacataca aatgatttta tgaaaaaaaa caagactcaa aaatttttat
    55561 attctttgat agaataaaat tgagagtaaa tttattgaat tttataaaat ttataagggg
    55621 tgagtctaag atatgcatga agataaaaat gatatatttg aatcaataga tactgaggat
    55681 tttgataata aaacagaaga agaatgttta gagctagtaa gaaaaaatgg attgaattta
    55741 atatacataa aaaatcaaac agaagagata tgtctagagg ctgttagaca aaattgtaat
    55801 gcaataaaat gtgttcagaa tcagacagaa aaaatatgta tagaagcagt taaacaagat
    55861 tggagaatgt tagaatttgt ggaagagcca acagaagaaa tttgcatgtt ggctattaac
    55921 caagatggaa cagcattaaa atatatagaa aatcagacag aggagttatg cttaagagcc
    55981 gttgaaaaaa atggtgcagc tttggagtat gtaaaagaac aaacagaaga aatatgtata
    56041 gaagcagtta gaaacagcga atttggattg gcaagagtga aaaatcaaac agaaaaaata
    56101 tgtatggaag cagttaagca ttgtagctat aatctaaaat gggtagaaaa tcaaacagaa
    56161 gaaatatgta tggaagcagt gagacaaaat gggcttgatt tgaaatttgt aaaaaatcaa
    56221 acagaaacaa tctgtttaag agcagtaaga caaaatggaa tggcattaga atttgtaaag
    56281 gaacagactg ttggtatatg cttaaaggca gtaagacaat atggaatggc attaaaattt
    56341 gtaaaagaac aaacagaaga aatttgtaca gaagctataa aacaagataa aagagcactt
    56401 agttttgtaa aaggagataa ggaaaaatac aaggctctat atgataataa tgaaccattt
    56461 gcaaagagat atgttagaaa tgtaatagaa aaagaaaaca gagcattgat taaaaaagga
    56521 gaagaaaatg caaaaatcaa aattgcagga aaactttatg atagaggcat gtcttttgaa
    56581 gatatatcag atatagttga gattgaaata agcaagttaa aagtgagttt aggtgttttt
    56641 taaagtaata aatgtaggtg ttttttatgg gaaatagtta ataaaaaaat aatcgactag
    56701 ctctttgtag gacagcaaaa ttagtgatat aatttgatgt ataataaatt atttttatta
    56761 gataaggggg aattaaagtt gaagattaca gtattatacc aaacaagaag tggaaacaca
    56821 gaaagggtcg ctaagctgat agaagatgga gctaaaaagg tggatggagt agatgtaaag
    56881 cttatgagac ttgataatat tgatttagat tatttaaatg aaagtaaagc agtaatattt
    56941 ggaacaccta cttatttagc aaatacaact tgggaagtaa aaaaatggtt tgatgaagat
    57001 tcaaaaaaag ttaatttagc tggaaaatta ggagctgttt ttgctacatg tgattatata
    57061 tgtggaggtc cagatgttgc gatacttact atagtaggac atttgatggt taaagggatg
    57121 cttgtgtatt ctggaggtgg ttctttagga aagccattta tacatttagg acatgtacat
    57181 tcaattgaag gtccagagtt acaagatgag aaggctataa tatttggtga aagaattgct
    57241 actaaggcaa aagaactatt tgcttaattt ctatatgcat aataatgatt agagtaaatt
    57301 tagggtttta atggagattt gaagtaaaag ttataaaata aaaaattttc cataaaatta
    57361 ctcaagatga attataatat aattataaat aaattatatt attaaaatat ttaaaatcaa
    57421 acgtcaatag cgagggaagt ttatggtatg gcatatagaa tatgagattt tttctgcaat
    57481 tatagttatt tttttaatgg tatacttttt tagaagtaaa tttataccta ctttacaaaa
    57541 caaaatatat tgtgcacttt taatattctc atttttattt ataatttcaa atattttagg
    57601 ttcattttgt ctaaataata tagacaaaat tccaattttt attacttttt tattgaatca
    57661 gatatactta ttgttattgc caataccagc agcacttgta tcattttatg ttatggctat
    57721 tatttatcaa gatataaggt atatgaaaaa aagattatta tttttatcta taccacttat
    57781 aatttgtata atcttatcta ttacaaatcc ttttacgcat attctatttg gattgtcaaa
    57841 agaaacaggt tatatacgag gttgtggata tgttgcaact tttgcatctt ttttctttta
    57901 tgcaatatat tcagcatttt tatcttttaa atatagaaaa gcaatgcatg aatcaaaaat
    57961 atgggctatt agaggttttc ttatagtttc aattgtggca attatcttac aggcaatttt
    58021 tttacagtat cttcttacag gaactgcatg tgcatgtagt ttacttttga catatctttc
    58081 tatgcagaat cgaggtctta ttattgatga ccttactggt gtactcaata gacaatcttt
    58141 tattcaagag ctagatttga atatcaatac tgaacaatca ggatttatta ttacaattgc
    58201 gcttgatgat tttaagttta tgaatgaaac ttttggaaca aaaaatggag atattgcact
    58261 agaagaagtt ggaaaatatt tgattcagat acatgatgta aaccatgtat atagaatagg
    58321 gggagatata ttttctattg ttgtcaatag tcaattaggt atagaaccag atgatattat
    58381 cagaaagata gaagaaagat ttaaaaagcc ttggctagta gaagagataa gttttaattt
    58441 agcaactagt attgctgttg tttattaccc agaaaatgca gaaacgacag aagatgtaat
    58501 tgttgctata gatttctcta tacatgaagc aaaacgctct gagtcaaggc gagttgttta
    58561 tgcagatgcg tctataagtg aaaagataaa gaagaaacat gttattaaaa attgtttaaa
    58621 agaagcatta aaaaatgatg ggtttgaagt gtactaccaa cctatatttt ctaatagcag
    58681 gggcaggttt acttcagcag aggcattact taggctagaa cacaaagagt taggttttat
    58741 acctcctgat gaatttattc caattgcaga acaaacagga ttgattaact caattggatt
    58801 agtagtcttt gagaaggtat gtagatttat agctagtgat gagtttgaag atttaaaatt
    58861 ggataatata gctgtgaatc tttctgtagt acaatgtatg caaaaaaact tagctgatga
    58921 tttactttat cttatgaata aatataatgt atcaccaact aaatttaaac ttgaaattac
    58981 tgagactgtg gctatgggct cttttaatat aattaaagaa acaatggaaa gattaattga
    59041 tttaggtgtt aagtttgcac ttgatgattt tggaattgct tattctggag ttactaatat
    59101 gctttcttta ccattttcag taattaagtt agataagagt ttgatttggt ctatgaatga
    59161 agatagtagg cataaactta cagttgagac gattatagca cttataaata aattgaatat
    59221 gaaggctgta gcagaaggtg ttgaaaccat taagtgtgct gaagatttaa tagctatgga
    59281 gtgtgaatat ttgcaagggt attatttttc taaaccagtt ccagaagatg tttttaaaaa
    59341 tttgttgaaa gaaaatactt ttacaagtta acacccttta tttttttaat tacaactttg
    59401 ttcattgaca ctgccagcga tatattataa aattaacaca ataataaata ttaaatttat
    59461 tatacatatt attaatatta attaatggag taaagattta ttatatattt tatttaatta
    59521 tcctaaagtg agaaaggaag atgtgacatg aataaaaaga aaatattagt gccaattgat
    59581 gggacagaaa gaagtatgca ttctttggaa tttataaaag gaatatttaa aaaagatgaa
    59641 gttgaagtag aaataatgaa tgtaaaagaa ctagtattta ttgatggcat atccctggct
    59701 gaagagataa aaaattctga aaatttagga agaagaatct tggataaagc agcagagata
    59761 atgggagagt atgatgttaa agtacatttt acttttggat atccaggaga tgagataatt
    59821 agaaaagcaa aagaagataa tgtagatttt atagttatga caaaatctac taaaaaaggt
    59881 cttacaagaa tgataggttc agtaacagca agtgtagtta aacaagcaaa atgtatagtg
    59941 atgattgtac cggagtagaa ttagaaaggt atttgaaata tgaataaaaa gatagtagca
    60001 gtttgtgaaa gtgaaagaag tttaaattct cttaagacag ctgcaaatga tttaggattt
    60061 gatttagttt atgaaattca agaaaatgat aatataaaaa atgaactttc tatttatgat
    60121 atagaagatg catccatagt gttatttgtt acaagtgatt ctatagaaag catagagaag
    60181 attgatagat ttatagatag agaattttat gaagttgatc caaagtatat aatagaggat
    60241 gcaaaatcta tagtaagtga aattttaata gacttaaatt aatcgtgggt gtcgcactaa
    60301 gaatagagcg acgccttttt atatatttta cacaagaaat aaagaggttg tatcaaaata
    60361 tagataagac tctttttcat tgttgaaatg atgaaaatat atttgatttc atacaaaaaa
    60421 gagtacctca taactaaaaa gttcactttg agacactcca tttttatatg ttagttgcat
    60481 ttaaggtaaa atataagttt aatcatgttg aaataactga attacatctt ctaaatgatt
    60541 gtacattgat gtataaccta agtaagagtc tttactatca aatgccatca ttatcttgtt
    60601 atggtggcta atcatatcct tgagagaatt tctatattta aaaccgaagt attggttaga
    60661 agaaactgtt ttaggtatta actcataata ttttataaaa attggattat gagaagctaa
    60721 aattatctct ttgtgaaatt gcaagtcagc atcataacat tcttctatca aaccatgaga
    60781 aaccttatcc aattcctcta ttgactttag gtaagtatat tttatttttt gaagttcttt
    60841 agatgtagca tttaaaattg ctagtttagt agcttcgctt tcgataagtt gtcttgctct
    60901 taaagattct acataaaatt cttgtatgtt atgattttta tagatttcac catcatcaaa
    60961 taggtcaact tttttaaatc cttcaactat aggacttgaa taaggcacag ctctaagtat
    61021 tccttcctta cagagtatat ttatggcatt tcttacaggt attctactta tgttaaatag
    61081 gtcagcaatc tttctctcac ttggtaattt actattaatt ttaaaagttc catttagaat
    61141 gtttaattta atataatcaa taattttttc agtggcattt aagcttttca taaaaactcc
    61201 taaacatata atttttttat gattgtatta tatcatagtt aagagttaaa tctacttttt
    61261 tataatatgt ataagacagg atagaggcca aaatagctat aatagccaag ataaagtatc
    61321 catattgtat tgaagtatat tccgatattt taccaactat cattggaaat acagacattc
    61381 ctatagcata aattgcttga aaaaaaccca ttgcagtaga tttctgatgt tttggtacat
    61441 ttttcataga ctcgctagta aggtaagaaa ggagtatacc tgtagatata cctggtagta
    61501 cttgtagtac aaatattata ttaatatttt ttattgttgg gactaaaaag caataggctg
    61561 aaactaaaat aaaaactaaa ggaatccaaa attttggtcc atacttagta caaaatttag
    61621 tagaagcaaa gccagaagaa gcaacagcac acaccatgta aaatatagaa gatagaccta
    61681 tgagggtaga actagaacct aagtttttaa gaatttgagt agtaaatgac attgttgtag
    61741 acatttgaat accctgttga attaatgcca taaaggaaaa aaatacaagt cttttatcct
    61801 tgcagataga taaatatttt ttaattgata ttttttcagg tatttttttt gaattatgat
    61861 tttttatatt taaggctaga atcgttccaa taactgaaga caatacactc atgatacata
    61921 aggctgtcat tcctaatttt tcataaaata aagtactcat aacaaatcca atcaacattc
    61981 ctagattgtt taccaatatt atcgtacttg tagctttttg ttgatgttct gtagaaaaaa
    62041 agttggtata gtgaaccatg taagaaatcc aagttgatga tgatattcca gataaaatgt
    62101 tagctataag aaaaccattg cctccaggag aaaagactct tataagagat gctaaaccag
    62161 caaagaaaat tccagacagt ataaatattt tatgtttatt tatagaatca gcaaatactc
    62221 caacaggaag ccttaataaa cattgtgata ttccatatgc acctattatt ataccaatca
    62281 tatttgtaga tacattacat gcacttaaat atggattttg gaaaggtata tatacatatt
    62341 gtgaaaacca gaaaaatgta acaatagaaa gtaaaagtaa tttttgatta gatatttttt
    62401 tcatattatc ctccatatgg tatttttata atgcaaataa tataccattt agatttttta
    62461 atcaagtact ttctctatta aaaaaaattt aaatatataa ttattattta caaaaaaatt
    62521 atactttata gttgaattaa gaaaacgctt atgatactat aaagttaaca aatttgaata
    62581 tataacagta gacgtgttac tggtaatgca ggcaagatcg aatcaatttt tatagattat
    62641 ctatagagta ctatgggtat aatctattta tttggttcga tttttttgtt taattggcca
    62701 taaaacaaaa agcctatagt tgaaaatgga ggatttttta tggattataa aaatatagct
    62761 caagaaattc tactaaatgt aggaggaaaa gagaatgtaa atgaagtgac tcattgtatg
    62821 acaagactta gatttaaagt caaaagcgct tctaaagtga ataaggataa attatctaaa
    62881 actgaaggag taataactgt tgttgagagt atgggtcaaa tacaagttgt aattggaaat
    62941 aaagtaaaaa aagtatatga cgaggttata aaaattgtac ctcaatcaaa taacgtaggt
    63001 aaaaagaatg aaagtcaaga aaaccaaggt ataattaatt ctatattatc agcagtagct
    63061 ggtatattta cacctacaat acctgctata gcaggggttg gtatgattaa aggtatttta
    63121 tcagttcttg ctatgtatta tatgaataaa aatggagtag atataaaaga aacgcaaagc
    63181 tatattatct taaatgctat ggctgattct atattctatt ttatgcctat tatactagga
    63241 tatactgcgg ctaaagtatt taatgcaaat aaaataatat ctatggtatt aggagctaca
    63301 ctttgttatc caacttttac tgctttgatg gcaggtgaag aatctgttag atttttagga
    63361 ttggcagtaa caaaagctac gtatacatca tctgttattc caataattat tgctatatgg
    63421 gctctttcat acgttgaaaa agtattagaa aagtatatac ctgagataat taaaataata
    63481 atggttccaa ctttatcatt ggttgtaatg ttacctgcaa cattattttt atttggacca
    63541 atcggaattt atataggaaa tgtcataaac ttttcttata agtatattta tgaattaagc
    63601 ccagcattgt gtggagcatt tgtaggtggg ttatggtgtg tgttggttat atttggtgct
    63661 catagagcat tattaccaat aggtataagt gatgttgctc aaacaggaag acagaatcta
    63721 ttggcgtttg caggagctgc aaacttttca caagcagggg ctgctctagg agtattcttt
    63781 aaaactaaaa atcaaggact aaaaacaatt tctatgtctg caactataac agctttattt
    63841 ggtataactg aacctgcaat atacggtgcc aatcttagat taaaaaaacc tatggtatgt
    63901 gccgtaatat gtggtgcatt tggaggtgcg ataatgggaa tgggtggagc ttatggtaat
    63961 gcatttgcaa accaaggtgt tttaactata cctgtatatg ctgaagcagg tgcattaggt
    64021 ttcttaagtt atctaggtgg ttgtgctata gcattttttg gttcagctat ttcaacttat
    64081 ctagttggat ttgaagattt ggaagagtct tctaataagg aaaattcatc tataaaagta
    64141 gaaacgaagg atggtgttat tgatataact tcacctgtag agggcgaatg tatagaattg
    64201 tcagaagtaa aggatgatgt atttgcttca aaagctatgg gagaaggtat agctgtgtta
    64261 cctacaaaag gagtaataac agcccctact gattgtgaag tagcttcttt atttccaaca
    64321 ttacatgcaa taggattaaa attagataat ggagcagaaa tgttaataca tgttggaatc
    64381 aatacagtag agttaaatgg taaatacttt acaaaacatg ttaatcaagg agatttagtg
    64441 aaaaaaggtg ataaacttat atcctttgat atagataaaa taaaaaaggc tggttatgat
    64501 gtaactacgc cagttatagt aaacaataca tttgattttg ggcaagtagt ttcttgtaaa
    64561 tctagttatg tttcaaccaa tgataatata atttcattag tattagctgg taattaagtt
    64621 aatttgacta gagataagta aattgattta aaaggaaata agtaaatcaa caattaaata
    64681 catctaaaag taagtttatt gcaatgaatt gttaaagaaa ataattaata tggagtaaat
    64741 ctattgcaat gaagtaacaa agaaaataat taatatagag taagtttatt gtaatgaatt
    64801 gttaaagaaa ataattaata tagggtaaat ctatctcaat gaaataacaa agaaaagagt
    64861 taatatagag tgaacttatt gcaatgaatc aacaaagaaa ataattaata aaaaggagaa
    64921 atatttatga atacaggatt tccaaaagac tttttattgg gagcatcatc ttctgcattt
    64981 caagtggaag gagcttggga taaagacaat aaaggaaaaa ccgtagccga ctataattct
    65041 tttaaaaagt ctcatttaca ggcagatact aaagtcgcat cagactttta ccataattat
    65101 gaagaagata ttgaattaat gaaagaatta gggatgaaaa catatcgttt ttcaatttca
    65161 tgggcaagaa taataccaga tggtgaagga gagataaacc agaaaggttt agaattttat
    65221 aataagataa tagacaaact aatagagtgt gatattgagc cattcgtaac tttataccat
    65281 tttgaccttc catttaaatt agtagagaaa tataatggtt gggaaagtag agaaactgtc
    65341 tatgcttttg aaagatttgc taaaatatgc tttaagcatt ttggagatag ggtaaagtat
    65401 tggcaacctc ataatgaaca gaatttaatt gttagagtgg aggagagaat taatatatat
    65461 gatgaaacag attcatggaa gatagataaa atgcgtgctc agatggatta taatctttgc
    65521 ttagcacatg cattagcagt caatgcatgt catgagatga taaaagaaag taaaattgga
    65581 gcagcagtat catcatctgt aacatatcct cttacttcta agccagaaga tgtatatgca
    65641 gctagaatga atgataattt taaagtgtac tatatgcttg atatgcatca ttatggagaa
    65701 tatccagggt attatatgaa gtatcttgaa aaaagaaata tagttcctca catggaagat
    65761 ggggataaag agatacttaa aaaagctaaa atggatttta tagctgtaaa ttattatagg
    65821 acaaattgtg cagaagcttt acctgaagat tcacaacatt catttggatt aagagaagga
    65881 actgttgatt ttagtatgta tggactattt aaaatgtcaa tgaatcctaa cttagaggcc
    65941 tctgagtatg gagcagcaat agacccatca ggtcttagag ttgccttaaa tgaatattgg
    66001 caaagatatc acttacctgt tataataaca gaaaatggtc taggagctaa agatatatta
    66061 gaagatggaa aaattcatga tgactatcgt atagactatt taagaagtca tataaatgca
    66121 tgtaaactag ctattgaaga tggtgtagag atgattggat attgtccatg gtcatttaca
    66181 gaccttttaa gttcttctca aggttttaat aagcgttatg gacttgttta tataaataga
    66241 actgaccatg aaattttaga tttaaaaaga atcaagaaag atagttttta ctggtataaa
    66301 gaagttatag agaacaatgg aatagtaaaa taaacataaa tatatattga gggttttagt
    66361 atgtacatta caaaggtatt aaataattcg ttgctacttg caaaggatga taatgatgaa
    66421 gaaataatat tgatggggaa aggcattggc cataattata aggccgggtc tgagttaaat
    66481 aaaaaagata tagaaaagat ttttgttctt catgatgaaa atataaaaaa gagtattata
    66541 caattggcaa cagaaataga tgcagaatat tttggtatag ctcaaatgat aattgcatat
    66601 ggaatagaaa aatataactt aaaacttatg aaccatatat atttggcctt aactgaccac
    66661 attgcctttg cagtgagacg ttttaaagaa ggtataatga ttgaaaatca ctatttgttc
    66721 gaaatcaagg aatttaatcc aaaagagtat gatataggaa aatatgctat tactgttttt
    66781 aaggaggttc ttggtttaga cctaccagaa gaagagattg gtaatatagc aggccatttt
    66841 ataaatgcac aacaagataa tccttatagt gatagaaata agagaagtgc taaaattgta
    66901 aatgctattt tgcaaatagt tcattatcac ttttctattg tatataataa agagtcattt
    66961 tattatagga gatttgtaat gcatctaaag gccttttcac aaagatttct ggctaatgaa
    67021 ccatcaagag aaaaaataga ttttatatat gagcaagttc aaaaaaattg taaggaagag
    67081 tatgaatgtg tgaaaaaggt aggagcttat atgtataagg aatactccag agaattacca
    67141 agacaagagg agttatattt gatgattcat attcataaga ttttaggaga attatgcgaa
    67201 aatagttaga atatcttaat atgctagtaa tattataaaa aattaaaaat taactgcact
    67261 ttaaatatga ataattgcat tacttaaggg ggttgtctta aaattgattt ttttaattaa
    67321 atttgaagct caactccttt tttaaacctt tatatcatta caactttaat gtttatacgc
    67381 ctgtacttta gatgtatatc tactttttac atttctcaac atgcaaacct accatttttt
    67441 gcagtatgtt aattgaaata aaccattgag ttaaccctaa atttattctg gacttatagg
    67501 aatagttcca aagatacttt aaaatatttt ctggaataat tgcttatttc acatatgttt
    67561 tattgtgatg ttttgattta ataaattctt caactctgta tatagtaaat gtattatcat
    67621 gtttgtttta ttcaagcaca cttataatac gcttgatttt ttcacaatga tgaaatatac
    67681 taatgggtgt tataggtata agaatcgcat tacacttgaa gaagtaagtg ataaatgaga
    67741 tataaatcct catatgataa actactattt cattgaggaa catattctaa gtgatgtcaa
    67801 gatactacaa tatgactaaa ttcctagtga taaaaaagtc tacgaatgag tgtagaaagg
    67861 ggaactaaga tgaataaagt tttaattata gatgatgata aagatctttg tattttgtta
    67921 aaaaagagta tttcaattga acagattgaa gcaaactatt gccattctgg gagagatgga
    67981 ctggttgaac tttcaaaaca agattatcag ttagtagttc tagacgtgat gatgccaggt
    68041 atggatggtt ttgaaactat ggaacaaata cgaaaatata gcagtattcc catcttaatg
    68101 ctgacatcaa aaagcgataa ttacagcaaa gtgcatggat tgcgagctgg tgcagatgac
    68161 tatctaacca aaccatttga aattacagaa tttactgctc gtgtactgtc tttaatccga
    68221 cgttacaccc gttttaatcc aaaaaatgag gagccacagc aactttcttt caaaggtttg
    68281 ttgattgacc ttgaccagca tacagttact accaatggcg aacctatgtt gcttccaccc
    68341 aaggaatttg gtgtacttct ttactgtgca aggcatcagg gtaagatttt gacaaaacag
    68401 cagatttatg aagccgtgtg gggtgagcca tatgtatatg atgacagcaa tatcatggct
    68461 attatcagtc gcctaagaaa aaaaattgaa ctagacccat catctcccat ctatttgcaa
    68521 acaataaaag gaattgggta tcaattcaac acggaggtat gaaaatggat gaacaaacga
    68581 taattctttt cttactcttg ctgtcgattg gtctactttt tctttccata tttgcttttt
    68641 ccaagcttgg gcatatttac aagcgcctaa aagatataga ggagatactg gctgatgtac
    68701 aaattggaga cgaaaatcgt aaaatactaa ttaagccatg tgatgttatg gctccattgg
    68761 tttatcagct taatgagatt gtctacgatt atgaaaataa gttactttct ctgaaaaagt
    68821 cggataaagc tagcaaacaa cttatgacaa gtttatctca tgatgttcgt acaccattaa
    68881 caactctgat tggttatctg gatgctgtac acagtggtat agttatcgaa caagagcggg
    68941 aggaatacct tgaaatcgct aggcgtagag cctacgacct caaggattac attgatgttc
    69001 tttttgactg gtttcggttg aactctgatg aatttacgct gtcaatagaa tctgtggaaa
    69061 ttgcagagtt gtcacgcaat attttgaaag actggattcc tatattccat gaaaagaaac
    69121 tgaattttga aattgatatt ccagaaaatc gactaatggt aaacctcgac ccagatggat
    69181 attcccgtgt ggtcaataat ctggttcaaa atgttcttgc tcacagtaag gctcggcaaa
    69241 tcaaaattac catgagtgag gattccagaa tggtattact ccgtgttgag gatgatggtg
    69301 ttgggattgc cagagaaaat ttaccacata tttttgagcg actgtataag tgtgataaag
    69361 gacgttcaga aaaaggaagt ggtcttggtc tatctattgt cagtcaaatg gtggagcgaa
    69421 tgggtggtca ggtttccgta gaaagtgaga ttggaaagta taccgtattt acagtgagct
    69481 taccattaat ataaaattgt ttttccccca tgtgacatgt atacacaggg ggattttttg
    69541 taagttataa agttgcaagg ttaatacaag gttgttgcaa ggttaatgta agattgaggt
    69601 ggtatgataa ctttaataaa caaaggaggc ttttattatt tatgaataat ttaattattg
    69661 aaacaaaaaa tctcactaag caatatggtg ctcaaaagag cgtttctaat ttgaatattc
    69721 acgttcagaa aggtcgcatt tacggattgc taggaagaaa cggtgcgggt aagacgacaa
    69781 ctatgaaaat gttattgggt ctaacaaaac caactactgg agaaattaaa atatttggtc
    69841 aggacattca caataacgag aagaagcttc ttcctcgaat tggatgtttg attgaatcac
    69901 cagggtttta tcctaatctg acaggaacag aaaatctcaa aatctttgca aggcttagag
    69961 gaataccaaa aaaaaatgca gtagaatcag cactggatgt agtcggtttg ccaaacaatg
    70021 acaaaaagtt attttctcaa tactctcttg gtatgaaaca gaggttggcg attgctcttg
    70081 ccattatgca tgacccacaa ctgttgattt tggacgagcc tatcaatgga cttgatccta
    70141 ttggtattgc agaagtaaga acatttattc gtgatctctg cacagaacga ggtaaaacta
    70201 ttttgatttc cagtcacatt ctttctgaaa ttgccttgtt ggcagatgac attggcatta
    70261 ttgaccatgg tattttgttg gaggaagaaa gtcttgccca attagaagaa aaaaacagta
    70321 aatacataca ttttgctgta tctgatgcag cacaggcagc aaggcttctg gaaacccaac
    70381 ttaacctgaa aaatttcaaa gtagcagata attacaatat tatgctatat gatacagatg
    70441 tttctgttgc agaaataaat cgtgctttca ttctaaacaa cattgaggta tcagaatctc
    70501 atttatgcga agatactctt gaagattact tcaagaaagt aacagggggt gaaggaattg
    70561 cttgatataa tttcatgtga attttcaaaa ctcaagcggt taaaatttat actgatttct
    70621 attttaggtg cttgcttatt tccaattccc gcaacaattt tgattgcaaa ggataacttg
    70681 ccctttgagc aattgttcaa attagttgtc aattttggat attttttgct attaccaatt
    70741 gtattaagta ttgttgctag ccaactattt tttatagaaa gagataatga tgtattaaaa
    70801 aatcttgcaa cagtgccagt gccaaaagga aaactggctc tggcaaaatt ggctgtgttg
    70861 ctgtttatat cattgttcta ctctgttgca gggttaggag ctactatcat tggaggtttc
    70921 attgttggaa tagtagaggg ggttgctgta aaattaggaa tgagcattgc ttttggaatt
    70981 atgttttttg ttactgtact tcctgttgtg gtactcattg tatatttcaa tcgcagctat
    71041 attttttcta ttatactttc ttttgtatat gctattttca attttagtat atccttaaat
    71101 attatcaatt ttgaaccaaa taacccaata ttaagtgtat tacctgctcc tgttataatg
    71161 cgttggtgga tggcattttg gggagatccc actggcgaat acacggcact tagacagcca
    71221 tatttgctct caactcctgc ttgtgtaggt attttatgtt tgataaccat tatagcagtc
    71281 ctgctgattt gtatgatata caaaaagcag gagaattagg aggtatctat gcttgatata
    71341 attactacag aatttcaaaa aataaaaaga tataatattc tatggattgg tgttgttgct
    71401 gttctgtttt cagcactatt ggcagttttt caggttgtaa gttcacacgg cagtgaccct
    71461 ctaacgtatg agagctttgc aaacggagtt atctggaata atttcagtct tagttttcca
    71521 tttgggatta cacttattgg aggatatctt attaaccgtg aatatacaga ccaaacctta
    71581 aaaaacatcc taactgttcc tatttcatta cgcaaattgc tagtgggaaa attaattgca
    71641 gttggaggaa ttactgtatt gttaagtctt ttcagcttct tatgcacatt tattttgggg
    71701 ttgttatttt gtaatatcaa tatatctgtt acactcgttg taaaatcctt agttcagatt
    71761 ttgtctgtta atctgtattg ttatgttgct gttttgccga ttattgctta ttttggtcgt
    71821 aaacaaaatg catttctgac aggtgtaggg attgcatttg tatacggttt ttgtggtgtg
    71881 tttgttgctg gaagaaatct gacagacttt tatccaatca cagcaggtct tggtctagtt
    71941 ggatacaaca atggtgctgg tactgtttat cagccactta tcggtgctat gactttgatt
    72001 gtaattctaa tcttgacaac tattctactt gtatttacac ctaattatga taaagttatg
    72061 accatatcta agaaaaaagg gataaagcat aaaaagtatt ctcataaata acataaatat
    72121 tgtttctatt atttaagttg ctagacagtg aaaagaaaaa atctgtcgta cagtggctat
    72181 atgctcatgt attcaagaag ctacagtttt gatttttcat aactgtagct tctttttgta
    72241 tttattaaat cacgactatc ataatacttg caataggtat agcgacaaga gagagtactg
    72301 tcatatgtgt tttatatatt acattaagta tattctaaaa tatatgtttt aaataatata
    72361 tttgaaaata ggaatattta aataaaaaaa ataatattat atatagttga caaaaattgg
    72421 caaatgagat actatataaa tatagaaata gtaatcatta ctattattaa aaagggagga
    72481 attaattatg aacttaaaag gaactaaaac agaaaagaat ttaatggcag gatttgcagg
    72541 agagtcagaa gcaagaaata aatatacata ctacgcttca aaagcaagaa aagaaggtta
    72601 taatcaaata gctgctatat ttgaagaaac tgctaacaat gaaaaggaac atgctaaatt
    72661 atggtttaaa cttttacatg atggtatgcc ttcaacagag gaaaatttaa aggatgcagc
    72721 agcaggtgaa aactatgaat ggactgatat gtatgcaaaa tttgctaaag aagctagaga
    72781 agaaggattt gacaagatag cttatttatt tgaagctgtt ggaaaaatag agaaagagca
    72841 tgaagaaaga tacttaaagc ttttagaaaa cttaaatgaa ggaaaaatat ttaaaagaga
    72901 tgaagaagtt gtttggcaat gtcaaaactg tggtcatgtg tatgtaggta ctgaagcacc
    72961 agaaaaatgc ccagtatgtg accatccaaa agcatatttc aatataaaag ctgaaaatta
    73021 ttaaaaatta tcattattaa aaaggaatgt taactatgaa attttctaaa caacgagaac
    73081 tgattttaaa tgaaatatta aataatccgg ttcatcctac tgcggattac ctatatgaaa
    73141 acttaaaaaa agataatcca aatttaagct taggaactgt gtatagaaat ttagctcagc
    73201 taacggaaca tggatttata agaaaggtta gtattccagg gtatccagat agatttgatg
    73261 gtcgaataga taatcactat catatcatat gtgaagtatg tggagaggta tacgatttag
    73321 aatccgaagt tcttaataat ttacaagaat taatatctga ggagacagac ataaaaataa
    73381 catcttataa cataagtttt aaagggattt gtaataattg taaaaggtgt agccaagtag
    73441 gttagttttc aaattctaaa aataattaat gaggtgagtt tatgtgtagt gaacaaaaat
    73501 tttttatatg tgagacttgt ggaaaccttg taggaatgat acaaagtggt ggtgtaccta
    73561 ttttctgttg tggaaaacct atgaaggaac ttgttcctaa tacaacagat gcagcagttg
    73621 aaaaacatgt acctgtaatt gaagtggatg gaaataatgt tactgttaaa gtaagtagca
    73681 caactcatcc aatgactaaa gaacatcata tagcatgggt gtatcttatg actgaacaag
    73741 gtggtcaacg caaatgtcta gcagtagatg gagaacctgt agttaaattt gctttgaatg
    73801 atgatgataa agtgatttct gcctatgctt actgtaattt acatggatta tggaaggctg
    73861 aactgtaata tttaaataaa ttgagtcaaa aaggaggcta taaatgtatt ttcatacaaa
    73921 tagcctccta taaatttata taaattcaag ttttaatttt gtttaatgtc aatataatat
    73981 aaggagaaaa taatatgtca atttataaat gtagtgtttg tggatatatt tatgatgaat
    74041 ctaagaatga taaaacatgg gatgaattaa gtgaagattg ggaatgccct gtatgtacaa
    74101 aaggtcgtag ttattttgga aagataagta ctgtttacta tgaagaagat gaaaagatag
    74161 cagaagacat agttgaagat gaatctaaat taaatactga aaaagaggga gatttaaatt
    74221 acttaagtac atatttaaga agagatgatg aagttgagaa acatatggat ataattcacg
    74281 aaatggctgt tacaggaaaa tctataatag aacctatgag aactaaacta ccagtaattt
    74341 cttgggatga tattttaatt atgggtgctc aattaaatcc attaccttta aatgagcatg
    74401 atgaagtaaa tacaacaaca ataataggaa aaaaagcaaa gaaacctatg attatagaaa
    74461 atccagttta tatttcccac atgtcatttg gagctttgtc taaagaacta aaaatagctt
    74521 tagcaaaggg tgcagcccaa aataagacag ctatgtgtag tggtgaagga ggtatattac
    74581 cagaagagaa ggaagcttca tacaagtata tttttgagta tgtaccaaat aaatatagtg
    74641 taactgaaga aaatcttaaa aattctgatg ctatagagat aaaaattggt caaggaacaa
    74701 aacctggtat gggaggtcat cttcctggcg aaaaagtaac tgaagaaata gcaaaagtaa
    74761 gaaataagcc tgtaggtcaa gatgtaataa gtccatcttg ttttgaggaa atacaatcca
    74821 aagaagattt aaagaaactt gtagatgaac ttagagaagt ttcagaaggg cgtccaatag
    74881 gtgttaaaat ttcagcaggt catattgaaa aagatatgga atttatagct tatgctaaac
    74941 ctgattttgt aactattgat ggtcgtggtg gagctactgg agcaagtcca aagttgctta
    75001 aagatgcaac ttcaattcca acaatatttg ctctttatcg tgctagaaaa tacatagata
    75061 cacatggatt agatatagat ttggttataa caggtgggct tcgtatatct actgattttg
    75121 ctaaggcaat tgctatgggt gctgatgcag ttgctattgc aagttctgct cttatggcag
    75181 cggcgtgtca acaatatcgt atatgtggtt ctggtaagtg tcctgtagga gtagctactc
    75241 aggatgagga attgagaaaa agattacata tagaaaacag tgctaataga gtggcaaact
    75301 ttttaaatgt aagtttagaa gaacttaaga cttttgctag aatttctggt cataaagata
    75361 tacatgattt aagtgttgat gatttatata ctgttaactc tgaaatatca aattatacaa
    75421 acatacagca tgtatagatt gaagtttgaa actattagat tttagatagc tataaaagta
    75481 aatgataaat atattttata aaagagagtg tcttaaaatg aactttttag ttcataagtc
    75541 actctttttt tgatgaagcc aaatatattt atcattttag caatgaaaaa gaatcttatc
    75601 tctttatttt gagatagact ccttctattt atgaaaatta aagatattaa attgtaaaat
    75661 attctgtgat gtcgtactat taaactaaag attctgtact ttaaagtaaa taaaaattta
    75721 aggagtgatt attatggaaa gattgaagat acaagtttta gttgacaata atacttatat
    75781 tgatagatac tttgttggag aaccagcagt aagctattat attgagattg atggaaatag
    75841 gattttgttt gactcagggt attcagatgt attcatatca aacgctgaaa aattaaatat
    75901 agatttagga aatttaactc atgttgtttt ttctcatggg cataatgacc atacaagagg
    75961 tatccagttt ctagaaaata ggtatgattt atctacagtt gaacttattt cacatcctaa
    76021 ctgttttata cctagaaaac atggagagaa aagcatagga gctccatttt ctgctgagga
    76081 aattaaaaat atttttatgt ataatcctaa ggataagcca tttaatctaa gtaagaattg
    76141 tgtgttttta ggtgaaatac cttctataaa tgattttgaa aaaagagcta aaattggtaa
    76201 atgtaaaata ggagatttgt gggaagatga ttatgtttta gatgattctg ctatagtttg
    76261 taaaactgat aaaggtattt ttattgttac aggatgttct catagtggaa tttgtaatat
    76321 agtagagtat gctaaaaagg tatgtggaga tgatagagtt attggtatac taggtggttt
    76381 tcatcttttt gaattgaata ataggttaga tagtacaata caatatttgg ataaagaaga
    76441 aatgagaatg ttgtaccctt gtcactgtgt ttcttttaag gtaaaagcga agatgaatga
    76501 aatattgaat ataaatgaag ttggagtagg gctgaccata agtatatagt atttgaaagt
    76561 atataagatt taagttgaag gttaaa
//
LOCUS       DAKBNN010000003        70077 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000003 DAKBNN010000000
VERSION     DAKBNN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 70077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.003"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            253..390
                     /gene="rpmH"
                     /locus_tag="OUB02_000177"
     CDS             253..390
                     /gene="rpmH"
                     /locus_tag="OUB02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCT8883995.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            468..812
                     /gene="rnpA"
                     /locus_tag="OUB02_000178"
     CDS             468..812
                     /gene="rnpA"
                     /locus_tag="OUB02_000178"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCT8883996.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            844..1095
                     /gene="yidD"
                     /locus_tag="OUB02_000179"
     CDS             844..1095
                     /gene="yidD"
                     /locus_tag="OUB02_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCT8883997.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            1127..1834
                     /locus_tag="OUB02_000180"
     CDS             1127..1834
                     /locus_tag="OUB02_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCT8883998.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1803..2432
                     /locus_tag="OUB02_000181"
     CDS             1803..2432
                     /locus_tag="OUB02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HCT8883999.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2544..3923
                     /gene="mnmE"
                     /locus_tag="OUB02_000182"
     CDS             2544..3923
                     /gene="mnmE"
                     /locus_tag="OUB02_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCT8884000.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4018..5913
                     /gene="mnmG"
                     /locus_tag="OUB02_000183"
     CDS             4018..5913
                     /gene="mnmG"
                     /locus_tag="OUB02_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCT8884001.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            5906..6625
                     /gene="rsmG"
                     /locus_tag="OUB02_000184"
     CDS             5906..6625
                     /gene="rsmG"
                     /locus_tag="OUB02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCT8884002.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6788..7573
                     /gene="noc"
                     /locus_tag="OUB02_000185"
     CDS             6788..7573
                     /gene="noc"
                     /locus_tag="OUB02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCT8884003.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7708..8481
                     /locus_tag="OUB02_000186"
     CDS             7708..8481
                     /locus_tag="OUB02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCT8884004.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENIDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8483..9346
                     /locus_tag="OUB02_000187"
     CDS             8483..9346
                     /locus_tag="OUB02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCT8884005.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9379..10521
                     /locus_tag="OUB02_000188"
     CDS             9379..10521
                     /locus_tag="OUB02_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCT8884006.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10655..11230)
                     /locus_tag="OUB02_000189"
     CDS             complement(10655..11230)
                     /locus_tag="OUB02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HCT8884007.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11490..12782
                     /locus_tag="OUB02_000190"
     CDS             11490..12782
                     /locus_tag="OUB02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCT8884008.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            12887..13489
                     /gene="yedF"
                     /locus_tag="OUB02_000191"
     CDS             12887..13489
                     /gene="yedF"
                     /locus_tag="OUB02_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCT8884009.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13562..13825
                     /locus_tag="OUB02_000192"
     CDS             13562..13825
                     /locus_tag="OUB02_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCT8884010.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            13840..14025
                     /locus_tag="OUB02_000193"
     CDS             13840..14025
                     /locus_tag="OUB02_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCT8884011.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTRCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14128..15324
                     /locus_tag="OUB02_000194"
     CDS             14128..15324
                     /locus_tag="OUB02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT8884012.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15538..15816
                     /locus_tag="OUB02_000195"
     CDS             15538..15816
                     /locus_tag="OUB02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCT8884013.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            15853..16287
                     /gene="ssb"
                     /locus_tag="OUB02_000196"
     CDS             15853..16287
                     /gene="ssb"
                     /locus_tag="OUB02_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT8884014.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16306..16533
                     /locus_tag="OUB02_000197"
     CDS             16306..16533
                     /locus_tag="OUB02_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCT8884015.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16676..16996
                     /locus_tag="OUB02_000198"
     CDS             16676..16996
                     /locus_tag="OUB02_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCT8884016.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17004..17960
                     /locus_tag="OUB02_000199"
     CDS             17004..17960
                     /locus_tag="OUB02_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCT8884017.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            17971..19965
                     /locus_tag="OUB02_000200"
     CDS             17971..19965
                     /locus_tag="OUB02_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCT8884018.1"
                     /translation="MSNKQAFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            19966..20415
                     /locus_tag="OUB02_000201"
     CDS             19966..20415
                     /locus_tag="OUB02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCT8884019.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20434..21762
                     /gene="dnaB"
                     /locus_tag="OUB02_000202"
     CDS             20434..21762
                     /gene="dnaB"
                     /locus_tag="OUB02_000202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCT8884020.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDLYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            21967..22275
                     /locus_tag="OUB02_000203"
     CDS             21967..22275
                     /locus_tag="OUB02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884021.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22357..23646
                     /locus_tag="OUB02_000204"
     CDS             22357..23646
                     /locus_tag="OUB02_000204"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCT8884022.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            23972..24047
                     /locus_tag="OUB02_000205"
     tRNA            23972..24047
                     /locus_tag="OUB02_000205"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24005..24007,aa:Lys,seq:ttt)
     gene            24054..24128
                     /locus_tag="OUB02_000206"
     tRNA            24054..24128
                     /locus_tag="OUB02_000206"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24087..24089,aa:Glu,seq:ttc)
     gene            24133..24208
                     /locus_tag="OUB02_000207"
     tRNA            24133..24208
                     /locus_tag="OUB02_000207"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24166..24168,aa:Val,seq:tac)
     gene            24242..24316
                     /locus_tag="OUB02_000208"
     tRNA            24242..24316
                     /locus_tag="OUB02_000208"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24274..24276,aa:Thr,seq:tgt)
     gene            complement(24699..25691)
                     /locus_tag="OUB02_000209"
     CDS             complement(24699..25691)
                     /locus_tag="OUB02_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8884023.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25700..26758)
                     /locus_tag="OUB02_000210"
     CDS             complement(25700..26758)
                     /locus_tag="OUB02_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCT8884024.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            26883..28346
                     /locus_tag="OUB02_000211"
     CDS             26883..28346
                     /locus_tag="OUB02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HCT8884025.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            28448..29245
                     /locus_tag="OUB02_000212"
     CDS             28448..29245
                     /locus_tag="OUB02_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8884026.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            29258..31360
                     /locus_tag="OUB02_000213"
     CDS             29258..31360
                     /locus_tag="OUB02_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HCT8884027.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            31428..31907
                     /gene="rlmH"
                     /locus_tag="OUB02_000214"
     CDS             31428..31907
                     /gene="rlmH"
                     /locus_tag="OUB02_000214"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCT8884028.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32056..32367
                     /locus_tag="OUB02_000215"
     CDS             32056..32367
                     /locus_tag="OUB02_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884029.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            32415..32681
                     /locus_tag="OUB02_000216"
     CDS             32415..32681
                     /locus_tag="OUB02_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT8884030.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNF"
     gene            32945..33646
                     /locus_tag="OUB02_000217"
     CDS             32945..33646
                     /locus_tag="OUB02_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8884031.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLFKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNENSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            33870..35171
                     /locus_tag="OUB02_000218"
     CDS             33870..35171
                     /locus_tag="OUB02_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8884032.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            35308..36402
                     /locus_tag="OUB02_000219"
     CDS             35308..36402
                     /locus_tag="OUB02_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCT8884033.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFLKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            36404..37537
                     /locus_tag="OUB02_000220"
     CDS             36404..37537
                     /locus_tag="OUB02_000220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HCT8884034.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            37555..38664
                     /locus_tag="OUB02_000221"
     CDS             37555..38664
                     /locus_tag="OUB02_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HCT8884035.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            38714..39469
                     /locus_tag="OUB02_000222"
     CDS             38714..39469
                     /locus_tag="OUB02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HCT8884036.1"
                     /translation="MERINMNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGV
                     LSKNYSTVEVAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFT
                     GVATSRALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMG
                     GSSIKFFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKV
                     IPHVYTSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            39474..40466
                     /locus_tag="OUB02_000223"
     CDS             39474..40466
                     /locus_tag="OUB02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCT8884037.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(40654..41535)
                     /locus_tag="OUB02_000224"
     CDS             complement(40654..41535)
                     /locus_tag="OUB02_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8884038.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            41809..43197
                     /locus_tag="OUB02_000225"
     CDS             41809..43197
                     /locus_tag="OUB02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884039.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            43225..44178
                     /locus_tag="OUB02_000226"
     CDS             43225..44178
                     /locus_tag="OUB02_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCT8884040.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            44238..44462
                     /locus_tag="OUB02_000227"
     CDS             44238..44462
                     /locus_tag="OUB02_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884041.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            44455..44967
                     /locus_tag="OUB02_000228"
     CDS             44455..44967
                     /locus_tag="OUB02_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCT8884042.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            44999..45853
                     /locus_tag="OUB02_000229"
     CDS             44999..45853
                     /locus_tag="OUB02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HCT8884043.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            46387..47181
                     /locus_tag="OUB02_000230"
     CDS             46387..47181
                     /locus_tag="OUB02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8884044.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            47285..48109
                     /locus_tag="OUB02_000231"
     CDS             47285..48109
                     /locus_tag="OUB02_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT8884045.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
     gene            48740..50827
                     /locus_tag="OUB02_000232"
     CDS             48740..50827
                     /locus_tag="OUB02_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8884046.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            50842..51129
                     /locus_tag="OUB02_000233"
     CDS             50842..51129
                     /locus_tag="OUB02_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884047.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            51156..52427
                     /locus_tag="OUB02_000234"
     CDS             51156..52427
                     /locus_tag="OUB02_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCT8884048.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            52485..53084
                     /locus_tag="OUB02_000235"
     CDS             52485..53084
                     /locus_tag="OUB02_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8884049.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            53089..53994
                     /gene="dapA"
                     /locus_tag="OUB02_000236"
     CDS             53089..53994
                     /gene="dapA"
                     /locus_tag="OUB02_000236"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCT8884050.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            54100..54732
                     /locus_tag="OUB02_000237"
     CDS             54100..54732
                     /locus_tag="OUB02_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HCT8884051.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            55210..57717
                     /locus_tag="OUB02_000238"
     CDS             55210..57717
                     /locus_tag="OUB02_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8884052.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            58059..60221
                     /locus_tag="OUB02_000239"
     CDS             58059..60221
                     /locus_tag="OUB02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCT8884053.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYGDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIALDKIISNSSFNKIIKGEH"
     gene            60226..60843
                     /locus_tag="OUB02_000240"
     CDS             60226..60843
                     /locus_tag="OUB02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884054.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            61032..61376
                     /locus_tag="OUB02_000241"
     CDS             61032..61376
                     /locus_tag="OUB02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCT8884055.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            61946..62812
                     /locus_tag="OUB02_000242"
     CDS             61946..62812
                     /locus_tag="OUB02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCT8884056.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            62925..63329
                     /locus_tag="OUB02_000243"
     CDS             62925..63329
                     /locus_tag="OUB02_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HCT8884057.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            63496..63945
                     /locus_tag="OUB02_000244"
     CDS             63496..63945
                     /locus_tag="OUB02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8884058.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(64101..64478)
                     /locus_tag="OUB02_000245"
     CDS             complement(64101..64478)
                     /locus_tag="OUB02_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT8884059.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            64740..65657
                     /locus_tag="OUB02_000246"
     CDS             64740..65657
                     /locus_tag="OUB02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCT8884060.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNVTLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            66141..67559
                     /locus_tag="OUB02_000247"
     CDS             66141..67559
                     /locus_tag="OUB02_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCT8884061.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            67635..68447
                     /locus_tag="OUB02_000248"
     CDS             67635..68447
                     /locus_tag="OUB02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8884062.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            68870..69487
                     /locus_tag="OUB02_000249"
     CDS             68870..69487
                     /locus_tag="OUB02_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCT8884063.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(69644..70060)
                     /locus_tag="OUB02_000250"
     CDS             complement(69644..70060)
                     /locus_tag="OUB02_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HCT8884064.1"
                     /translation="MVYPISSTAPIRFRRYTVSYLENMENIYLTIATYFDTLGLGTMN
                     IDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVAITSIVNGDRVFL
                     ISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
BASE COUNT        27660 a         7517 c        12382 g        22518 t
ORIGIN      
        1 atgttaaaat tactttattt gtgtatataa ttgaaaaaaa taatttagaa taaaattaaa
       61 tagattttta ctagtatttt caataaaaaa aatacataat atatatatgg tagattttgt
      121 gatataagcg tcccaaaaac tcgttgacaa cagtagtaaa agtatttata attatatgtg
      181 ttatgtttac tttgagggta aagttattat ttttataaac ggaaaataaa atctgaaagg
      241 cggtgcaaaa taatgagtaa aagaacttat caaccaaaaa agagacaaag aagtaaagaa
      301 catggtttca gaaaaagaat gaagacgtct aacggaagaa acgtgttaaa gagaagaaga
      361 gctaaaggaa gaaatagatt aactcattaa ttacaaaagg gccgctgcaa aggtggcctt
      421 tttgtgtaaa taattacaat tcatagctgt gtttatggag gttaactatg gactttaata
      481 ggactaaagg gttgaaaaaa gactctgatt ttagaaaagt atataaacac ggcaaatctt
      541 ttgcaaacaa atatttagta atatatatac ttaaaaataa atcagattat agtagagtag
      601 gtatttctgt ttcaaaaaaa gtaggaaaag ctattactag gaatagagta agaagattaa
      661 taaaagaagc ctacagatta aatattgatg aaaaaattaa gcctggatat gatatagtat
      721 ttatagcaag ggtatctagt aaggatgcta cttttaaaga catagacaag tctataaaaa
      781 atttagttaa aagaacggat atttctattt aattgtatgc tataaatatg gaggttaatg
      841 actttgaata aaataattta tatattaaag gaaataagta agtatttatc gaatttatgt
      901 atttatttag tgagatttta tcaaaagtat atatctccct taaagggacc tacctgtaga
      961 ttttatccaa cttgctcaca atattcaata gaggcgttta agaaatatgg atttgtaaaa
     1021 ggtatgtatt taacgataag aagaatttta aaatgtcatc cttttcatcc aggaggttac
     1081 gaccctctaa aataaaagag agtaactatt aggaggtata aaaaaattga atataatagg
     1141 aaatttctta ggattgattc ttaaggtaat atttgaattt gttaatcatt atggtatttc
     1201 tataatactt tttacgatac ttgtaaagat tattttatta ccattaacaa ttaagcagac
     1261 taagtcaaca aaggcaatgc aagatataca acctcgtata aaagagatac aagaaaaata
     1321 taaaaataag ccagaaaaac aaaatgaaga aattgttaag ttatatggag aagctaaaat
     1381 taatccacta tctggttgtt tacctttatt aatacagttt cctatattaa taggattatt
     1441 tagcgttttg agagaaccag ttgctcatgg cgtttttgca aacaaagctg catttttagc
     1501 agctgacaat gggtttttat ggataaaaag cttaacatca ccagattatg tattagcagt
     1561 attttcagga gcaagtgcat atgtcatgca aaaagtaatg acaccaaaag atcagttaca
     1621 aggctctatg aaagtaatga catatgtaat ggcaggtatg tctttctatt ggggatttat
     1681 attcccagca ggacttacat tgtactggac agtaagtaac ttattctcaa tagctcaata
     1741 ttacttaata atgaatcctt taaaagctaa actggctgct aactctaagg aggaaaaagt
     1801 taatgagaaa aagcctaaaa ttaataaaaa gtaagagtaa agaggaagct ataagtaaag
     1861 ctatagcaga gttgaattta aaagcagagg atatagaagt agaaatactt gaaaatccaa
     1921 gtaaagggtt tttaggtttt ataggtgcta aagatggtac ttatgaaatt tttgttatcg
     1981 agagggaagt agatgtagct aaaaatttta tagaggtaat gttgaaaaat gcaaatgttg
     2041 atgccaaagt caatgtatct caaaaagata atttaatcat ggttgatatt gaaggcaaag
     2101 aagctgcaag tttaattggt agaagagggg aaactttaga ttctattcaa tttttgactg
     2161 gattagctct taataaaata aataaagatt ctcatacaag agtacttgta gatatagaaa
     2221 attatagatc aaaacgagaa gaatctttaa taagatatgc taataaagtt gctagagaag
     2281 tagcgaaaac aagaaaaact aaaaaattag attatatgaa tccatatgag agaagaataa
     2341 tacattcagc tcttcaaaat gacaaatatg taattacata tagtgaagga accgatccat
     2401 atagaagatt agtaattgag tgtaaaagat aaagtcaacc tcctaaacat tagggggttt
     2461 tattttttaa tattattaaa tcattaatga ttggttaaat gtaaatctat atgtaaaaaa
     2521 ttatgaatta agaggtgata aatttgttta tagatgatac aattgcagca atagctacag
     2581 cacctggaga aggtggtata ggtatactta gaataagtgg agaaaaagct ttgaaggttg
     2641 cagaagaaat ctttaaatct atgtctggaa agagtattga agaatacaat aagagaacat
     2701 taatatatgg aaatatagtg gataatgaaa atataattga tgaagtttta ttggcatata
     2761 tgaaaggacc aaattcctat acaggagaag atgttataga aattaattgc catggtggat
     2821 ttatttcggt aaagaagata ttagaattaa tactatcaaa agatgttaga ttagcagagg
     2881 ctggtgagtt tactaaaaga gcattcttaa atggaagaat agatttatct caagcagagg
     2941 ctgtaattga tgttataaaa gccaaaactg atatagctca tgaagttgct caaaatcaat
     3001 tagaaggttc tttatcaaag aagataagag aattgagaga taaagtcact gagatattag
     3061 ctcatgtaga agttgctata gattatcctg aggaagatat tgagcatata acatatcaaa
     3121 ctcttaaaga aaaaactgat gaacttaaaa aagacattaa aaaattatat gatacagctg
     3181 aaagtggaaa aatacttaga gaaggcttaa agacggtaat tgttgggaaa ccaaatgtag
     3241 gtaaatcttc actacttaat tctattttag gagagaatag agcaatagtt actgatatac
     3301 caggaactac tagagatgtt atagaagagt ttgtaaacat aaaaggtata cctttaaaaa
     3361 ttgttgacac agcaggtata agagatactg atgatattgt tgaaaaaata ggtgttgaaa
     3421 agtctaaaga atcatttact agtgcagatt tgatagtaat ggttttagat gcatctagaa
     3481 agttgtcaga agaagatata gagatattag aaaaacttaa ggataaacaa acaatagtgc
     3541 tgttaaataa gaatgatttg aaacaagaga ttgaagaaga aaaaatactt aaatatgtag
     3601 aaaataatag tataataaaa atatcagctt tacaacaaga aggaatagaa gaattgcaag
     3661 ataaaataga gtctatggta tataaaggta gtatcaaaaa taattctagt ttagtagtaa
     3721 caaattcaag acataaagat gcacttagta aagcatataa atctgcaaca gatgctttaa
     3781 ttgctttgga acaaagtatg ccatttgact ttgtagaagt cgatttaaaa aatatatggg
     3841 actatcttgg atatattaat ggtgatacag taacagagga tttactagat aatatattcc
     3901 ataatttctg tattggtaag taatgtacaa aagtaaactt gtttttatta tagagatttt
     3961 taaaaaattt gaagtgtttc tcatgaaaca ttaattttga aaagaaagga attataaatg
     4021 ataaagtttg aagcaggaaa atatgatgtt atagtagtcg gagcaggaca tgctggatgt
     4081 gaagctgctt tggctactgc aagaatggga tataaaactt tgataataac aatgtctttg
     4141 gattccatag cattaatgcc ttgtaatcca tcaattggag ggactggtaa aggtcaatta
     4201 gttaaagaaa tagatgcctt aggcggtcag atgggattaa atatagacaa gacatatata
     4261 caaagtagaa tgttaaatac tgccaaaggt ccagcggtac actctttaag agcacaagca
     4321 gataaattta aatatcatga agaaatgaaa aagacattag aagatgagcc aaatcttgat
     4381 atagctatgg atgaagttgt agaaatactt catgaaggaa atgttgtaat aggagttgga
     4441 actaaactag gatgttcatt taaatctaaa gctgtaatat tggccactgg agtttatctg
     4501 aactcaaaga tatatatggg tgaagttgca ttttatgaag gtcctaatgc attggggtat
     4561 gcaaaatatc ttacagatag cttagttgag cttggcctta gaatgagaag atttaagacg
     4621 ggaactccag caagagttca tagagatagt atagacttct ctgtaatgtc tttacaagag
     4681 ggtgatgaaa aagtaactcc attctcattt atgaatgaaa atatagaaaa aaaacaagaa
     4741 ccttgttatt taacaagaac tacagaagaa actcaaaaag ttatactgga taatttaaaa
     4801 aggtctgcta tgtatagtgg agtaatagaa agtactggtc caagatactg tccttctata
     4861 gaagataaag tagtaagatt tagtgataaa acatcacatc aattatttat agaaccagaa
     4921 gggttaaata ctaaagaaat gtatatacaa ggaatttcaa ctagtttacc ttttgaagtt
     4981 caattagata tgtacaaaac tataaaagga cttgaaaatt gtaagataat gagacctgca
     5041 tatgctattg aatatgattg tgttgaccca actcaactaa aaataagttt ggaaataaag
     5101 ggtgttgaga acttatttag tgcagggcaa tttaatggaa cttctggata cgaggaagca
     5161 gcagctcaag ggcttatggc tggtataaat gcagttagaa agatagaagg aaaagagcca
     5221 tttgtattag accgttcaga agcttatata ggtgtcttat tagatgacct tgttactaaa
     5281 ggaactaacg agccttacag aatgatgaca tcaagagcag aatatcgact atatttaaga
     5341 caagataatg cagatatgag attaactcaa aaaggttatg atataggatt agttaaaaaa
     5401 gatagatatg aaaggtttct aaataaaaag gctgctgtag agaaagaatt tgaaagacta
     5461 aaaaatgaaa gagttacacc aaaagaagta aatagtttgt tagaagaaaa aggagcaaca
     5521 ccaataaagg ttggaatatc tctatatgaa tttttaaaga gaccagaggt tacttacgaa
     5581 ttgcttgaag aattagggaa aggcgctgga gatgatgttt caagagaagt taaagaacag
     5641 tgtgtaataa taactaaata tgaaggttat atagagaaac aattgaaaca aatagaccaa
     5701 tttaaaaaac ttgagaataa aaagctggat gagaaaataa attattcatc tatagaaggt
     5761 cttagattag aggctagaca gaaactagat gatataaaac ctatttcaat agggcaagca
     5821 tctcgtatat ctggtgtttc tccagctgat atatcagttt tacttatata cctagaacaa
     5881 atcagaagaa ctagaggtgg caaaggtgaa taatatagaa cttctgaaaa acgggattga
     5941 aggttttaat atagatacga atgacagtat gttagaaaaa tttaaaatct atagagagat
     6001 attagttgac tggaataaaa agatgaatct tactggaata gaagatgaaa aagaagtgta
     6061 tataaaacat tttttagact ctatttcagc tgtaaagaat ggatatataa aaaatgggat
     6121 gtctataata gatgttggta caggtgctgg ttttcctgga ataccattga aaatatgttt
     6181 agatagctta gaacttactt tgttagattc gttaaataaa agaataaact ttttagagga
     6241 agttagtaga gtactagagt tagataatat tacatttata catggaagag ctgaagattt
     6301 tggtaaaaat gaagactata gggaaaaata tgatgttgct acggcaagag ctgttgctgg
     6361 attaccgatt cttatggaat tttgtgtacc atttgtaaaa gttggagggt attttatttg
     6421 cttaaaaggt ccgaatgcta atttagagct agaagaatca caaaaggcta ttgatgtttt
     6481 aggattggag tatattgaaa aaatagatgt agagttacca gaggtagatt taaatcataa
     6541 tatattagtt tttaaaaaag tgatggaaac accagttaag taccctagaa aagctggtaa
     6601 accagccaaa aatcctatta agtaatgaaa aatttattat tggtaagctt aattatttct
     6661 caggaaataa ttaagcttac tgtaaaaaaa caaaaataaa ggaattatta aagtagatgc
     6721 agaaataaat ttgtatgtac atatagagaa caaaaataat ttatatctaa aataagagag
     6781 gtatgttatg gaagataaaa gagttatgga gatacctata gaagatgtag ttccaaatcc
     6841 atatcaacca agaaaaatat tttcacaagt ttcattggag gaattgagca attctataaa
     6901 ggtatatggt ataattcaac ctataacagt tagagcaaaa gatggcaaat atgaactaat
     6961 tgcaggagaa agaagattaa gagcagctaa attagcagaa cttaaaacca taccagcaat
     7021 tataaataat atgaacgatg aatcttctgc tgttctagcg ttattagaaa atttgcaacg
     7081 agaagactta aatttcattg aagaagcaat tggttatgaa aacttaataa aagaacatgc
     7141 ttttactcaa cagcaattag cagaaaaatt gggcaaaaat caatctactg tagcaaataa
     7201 gcttagaata ttaaagcttc caaacgatat aaaaatgaag ctaatagaga ataatttaac
     7261 agaaagacat gctagagcat ttcttaaact accaagtgaa gatttaatgc aaagtgtgtt
     7321 agacaaagtc ataaaaaatg aattgactgt taaaaaaaca gaaaaattga tacaagatgt
     7381 attagaagaa actaaagttc aagaagagcc tgataaaaaa cagaatataa agggtgctat
     7441 gagtataaga atttacataa atactataaa gcaggccttt gatgctatat caaatacagg
     7501 aattgatgct aagtataatg aaatagataa gggagattat atggaagttg ttgtaaaaat
     7561 ccctaaaaaa tagagattac tgcttaccag taatcttttt ttgtaatata agccttgtaa
     7621 tataaactga tttatagaca ttatttatac ttaaaaaaca cctattcaac atttaaaatt
     7681 aatttaattc ttaaaggggg gaacatcatg ggcaaagtta tagctgtatt caatcaaaag
     7741 ggtggagtcg ggaaaactac aacaaatgta aatttaagtg ctagcttagg aactttggga
     7801 aagaaaatat tagtattaga tttagaccca caaggaaata caactagtgg atatggtatt
     7861 aataaaaacg aagtagaaaa tacaatctat gaaattatgt tagatgggtt acatattaaa
     7921 gaagcaataa taagtactga atttgagaat atagatgtag ttccatcagc tactgaacta
     7981 tcaggtgcag agatagagct tacatctaaa actaatagag agtatatttt aaaaaattcc
     8041 ataaaagcgg taatagatga atatgattat atatttttag attgtccacc ctcactgggg
     8101 atgcttacaa taaattgttt aactgctgta gatagtgttt taattccaat acaatgtgaa
     8161 tattatgctt tagaaggtgt tagtcaatta atggagacta taaaattagt aaaatcaaga
     8221 ttaaatgctg atatagaaat tcaaggtgtt gttttaagta tgtttgatgg aagagctaat
     8281 ttatcaatac aagtagttga agaagttaag aaatacttta aaggtagtgt atatacaact
     8341 ttaattccaa gaaatgtaag acttgcagaa gctccaagtc atggaaaacc tgtaatttat
     8401 tatgataaaa gatgtagagg gtctgtagct tatttggagc tagcagaaga atttatagat
     8461 ttagaagagg aggaatggta gtatggagaa taagtctaaa agaagtaata gattaggtag
     8521 aggtcttagt gcattgatac cagaaattaa aggagaaact tcagaaaaag aaatagtcaa
     8581 catagatata gacaaaatat atccaaacga agttcaacct agaaagcaat ttgatgaaga
     8641 aaagataaaa gtattgtctg actctataaa aaattatggt gttttacagc caatagtggt
     8701 taaaatggat gaaaataata aatatatgat aatagctgga gaaagaagat ttagagcatc
     8761 aaaacttgca aataaaaatc aaatacctgc tataattaaa gatatagata tgaaagatat
     8821 aatggagata gccttaattg aaaacttaca gagggaagat ttaaattcaa tagaagaggc
     8881 tctagcatat aagagtttaa tagagcatta caatgtaact caggaagaaa tatcagaagc
     8941 tgttgggaag agtagacctc atataactaa tacactaaga cttttaaatc ttggacaaga
     9001 tgttatagaa atgatagaca gtggtagaat tacagcaggc catggtaagg ctttattgag
     9061 aattgcagat aaagatttgc aacttcaaat agcgaaaaaa atagaagaag aagaactttc
     9121 tgtaagagaa gtcgaaaata tagctaaaaa aatatctgaa aataaacaag aagaacctaa
     9181 aaaatctaaa cctaaggatg tatttatttt agatgttgaa gataaactta gaaatatatt
     9241 tggaacaaag gtgaatattt ctaaaggaaa gaaaaaaggt aaaatagaga tagaatacta
     9301 taatgatgat gatttaaata gtattgtatc aatgcttctt gaatagaaca aaaatataaa
     9361 acagagagga agattgttat gatttattta gataatgctg caactacata tccaaaacca
     9421 gaaagagtgt ataatgctgt tttagattgt atgaaaaatt attgtgcaaa tccaggtaga
     9481 gccggccata aacttgctat gagagctgct agggagatat atgatacaag agagaatata
     9541 gctaagttgt tcaatgtaag taatcctatg aatatagtat ttacatctaa tgcaacagat
     9601 tctttaaatt tagcgataaa aggtgtatta caagaaggag accatgtaat aacaacgtct
     9661 atggagcata attcagtaat aagacctata aaagcattgg aaaaaagggg tattgaaaat
     9721 actgtagtaa aatgcgatta tgagggattt ttagattatg aagatttaga aaaatcaata
     9781 aagaataata ctaaattgat tgtaacaaca catgcgtcta atgtatgtgg aactttaata
     9841 gatataaaaa aagttggaga aatagctaaa aaacataata tactattttt agtagatgca
     9901 tcacaaacag caggtgttta tgatatagac gtaaatgaat gtaatataga tatgttggca
     9961 atgccaggtc ataaatgctt atttggacct caaggaacag gtatattata tgtaagagaa
    10021 gggttgaact taaatatatt aaaagaaggt ggaacaggta gtaaatcaga agaaatagtt
    10081 caaccagagt tattcccaga taaatatgaa tcaggaactc ataatactcc aggtatagca
    10141 ggtcttaacc aaggtatact atttatattt gaaagaggaa tcaacaatat aagacaacat
    10201 gaagaagaat tatgtcaata tatgatagac aaattagagg aagtaccaga tataaagata
    10261 tatggaccaa aggacagtaa aaaaagagca tcagtaatag ctctaaatat aggagatatg
    10321 gattctggag aggttacttt cttattagat agtgattata atatagcgac tagatcagga
    10381 atacattgtt caccactagc acataccact ttaggtactt taaaacaagg tgcagtaagg
    10441 ttcagtatag gatatttcaa cacaaaagat gagatagata aagcagtgga agctttgaaa
    10501 aaaatttcaa aaaataaata gcatgagatt taataatata gaaataaaaa aaggggatat
    10561 ttcaaaataa aaatttgaat catctccttt tttgtacaaa aaagaaaagg ggttcctaag
    10621 aacctctaat ctatttgata atagtttcaa gaaattactt taagtaatct ccttcttcaa
    10681 gaactatatg accactttcg taattagaac catcaacagt aatttttatt tgttcaacat
    10741 tcatattttt tatgtaagta cgagcaactg aatcaagcat taaagtttct gcatcactac
    10801 ctaagtttga ctttgtaaag tcagagctaa catctaatat accagttttt acaccatcaa
    10861 tgtcttttgt agtaaatgaa tttaattttg cagattctgg aacaacttta agtttttgaa
    10921 gttcttcaaa taaagtgttc tcatcaattt tatttaaatc aactttattt tcaattaaat
    10981 ctgtcttatc tacatcaaaa gagtaaatag tagctgattg agctttagtt tcttcttttg
    11041 gagtaggttt agctttgtta ttaggaacct ctttttcagt attgttttct tcttttggtt
    11101 gctcaacatt tgtattgctc ttagtttctt ttttagatga atcacttcca ttttgagcat
    11161 tagtacaacc aactgcgaat atagatatag ataatatact taacactaac attattttct
    11221 tatttttcat aaaaatttct cctctcttaa aaactattct tatatataat tatagtcgtt
    11281 atgagcaaat atatgaatac aaaatagtaa caaatttaaa aaatgattta tttaaattaa
    11341 taaaactcaa aataaactaa attttagtca tttttatggg aaatcattta atgtacagtg
    11401 aaaatattgt aaaataaaag tagtgttttt aaaataaata taagttataa ttatattata
    11461 aaaaaatgtc ttcaaaaggt gggaatagta tggaaatttt gagcttagga gaaaagatta
    11521 aaaagttaag aaaagaaaaa aacatgacat tgaaagaatt agctggagat aggataacag
    11581 cagcacaaat aagccatata gagagagata aatctcatac tagttatgaa ctgttggaat
    11641 atctaggcga taagctagat gtaagtgtag aatacttatt agagaccaag gaaatgcaat
    11701 caaaaaagat aactgataat ttaatactac agagcgaaat ttatataaaa gaaaatgaat
    11761 taaacaaggc tgaggaccaa ataaaagaag ttttagatat atgtaagaga tacgatttaa
    11821 tggagaacta tggcagatgt aattttttaa tggctaatat taatttgaaa aaatctaatt
    11881 ataatgatgc tatagaaaac tttgaaaagg cattgtactt ctttataaaa aatagtgata
    11941 gtgaaaatat acttaagtgt tatttaaaca tagggaaaat atatatgcag gaagactttt
    12001 ataaaggtgc tataagccac tttaattttg cagaggaaat tttgtcagag agtaaatttg
    12061 aggatgttaa catacacaag gaactatata gtaagatggc atattgttac ataaaactag
    12121 ataatccaaa tatgtcattg aagtacatag aaaagataaa tgaaatagat aatagaaatg
    12181 atagaaaaga agatgtcgat attttggtgt taaaagcaaa taatatgctt gagattggaa
    12241 aatatgaaga atcaagagat tactttaaaa aagcactaaa aatacttgag agtgaggata
    12301 ataaaactgg attagcaaat gtgtatttaa caatttgtga tgtttacaga aaaataggtg
    12361 aaacagatag agtacttgaa tattcacaaa aggtatatga cataaagaaa aatgatgaag
    12421 atgaatacat gatgtcagga ttatttaaga taatagaagc atatatagat aaagaagatt
    12481 atgatttagc tagaaaatat tgtaagatag ccttagcttc atcaataaaa aataaaaata
    12541 aattcaatga atataaagca ttgagattct atgctaatat gtataaaaat cagaatgaag
    12601 taactcttgc aattgaatat ctgattaaat gcataaaaat agtatcagat ttaggcaaca
    12661 atagaatatt ggccaattta tatatagact taggtcaatt atattcaaat atatcaaaag
    12721 aaaaagagtt agaatattat caaaaaggtg tatttatgta caaaaactta gaaataatgt
    12781 agggtaaata aacctataag ggtatagaaa caatctataa caagcaagat aatatctatg
    12841 ataaaatata aattgtatga atatttttaa ggagatgaat cagacaatga gtataaaaat
    12901 agatgcgaga gggcttgctt gtcctaaacc agttataaat acaaaaagag aattggataa
    12961 cctagaagaa ggtgttgtag ttacaatagt tgataatgaa acagctaagg agaatatatt
    13021 aaaacttgct aaatctttaa gttgtgaagc aaatattgtt gacgaaaaag agaattttat
    13081 atctatagaa attagaaaag gtaaaaatat actagataaa caaaatgttg tagataataa
    13141 agaaagtgaa ttagacaata catgtatatt tatatcttca gataagatgg gacttggaaa
    13201 tgacgaatta ggaaaagtgt taataaaagg atttatatat acattaacag aatctaaacc
    13261 ttatcctaag tatgtacttt tagttaatgg aggagtaaaa ttaagtgctg aaaatgaaga
    13321 aaccatagaa aatctaaaaa tacttgagaa tatgggtgta gaagttttat cttgtggtac
    13381 ttgtttagat tattataatt taaaagacaa attacaagta ggtacagtta caaatatgta
    13441 tactatagtt gagacattaa agaatgcatc aaataccatt tctatataat gtatgtatgt
    13501 ttaataatat aaaactaaca ataaagatta actcaaatag atagtcagaa aggattaagt
    13561 aatgaatcaa atgtatatag tgtcatttaa ttcgacacat catgcgataa gatcagaaaa
    13621 gttatttggt gaaaatagtc taaaagtaat ggctcttcca acaccaagag aaataacagc
    13681 aagttgtgga atatcaatta aattttcttt tgaagatatg gaaaagataa aaactatttt
    13741 ggttgaaaat aatgtagata taaaaggaat ttattgtata agtaaattag aaaatggttc
    13801 aaaagaagta gagaagttag attaggaggc taattattta tgccaatgga tttaaaaata
    13861 ggtgatattg ttgaattaaa aaagcaacat gcatgtgggt gtaaagagtt tgaaatcata
    13921 agaacgggaa tggatataaa gataaaatgt actagatgtt caaggctaat aatgcttgat
    13981 agagagacct tagaaaagag agtaaaaaag ctaataaaaa agtgataaaa atttaacact
    14041 taaaaaatgt caaataccgt atattaatat ccaaaatttt ggtataataa gaattgaaga
    14101 aaacattttc atgggggtgc tttaattatg gcagttaaat atgcaaaaag aatgcaagga
    14161 ttacaaggat cagaaatacg tgagctttta aaacttactc aacaaccaca aataatatct
    14221 tttgctggtg gaatgccggc tccagagtta tttccagttg aggaaatgaa aaaagtatca
    14281 gttgcagtgc ttgaagaaaa tggaagatca gctatgcaat atactacaac agaaggatat
    14341 gaaccattaa gagaaaaaat agcagctaga atgaatgata aaaacaaaac taatgtaaac
    14401 aaagatgata tattagttac aagtggttct caacaaggtc tagattttgc aggaaaagta
    14461 tttattgatg agggtgatgt aattttatgt gaaagtccat cttacatagg agctataaac
    14521 gcatttaaat cttatcaacc taaattcata gatgttccaa cagattctga tggaatgata
    14581 atggaagaat tagaaaagat actagaaaca actgatagaa taaaaatgat ttacgtaata
    14641 cctgacttcc aaaatccaac aggaagaact tggccacttg aaagacgtaa aaaattcatg
    14701 gaaatagtaa ataaatttga aataccagta atagaagata atccatatgg agatttaaga
    14761 tttgaaggag agactttacc atctttaaaa tcaatggata caaaaggatt agtaatattc
    14821 ttaggaactt tctctaaaat attctgccca ggatacagat taggatggac ttgtgcttct
    14881 ccagaaattt tatctaagtt taactttgca aaacaaggtg cagacttaca agcatcaact
    14941 atatctcaaa tggaagtaag caaatttatg gatatgtatg acctagatgc acatgttgac
    15001 aaaataaaag cagtttatgt taaacgtaga gatgtaatgc ttaagactat ggaagaagag
    15061 ttcccagaag gattagtatt tactcatcca gaaggtggat tatttacatg ggtagagctt
    15121 ccaagtaatt taaatgctaa agaattaatg ccaaaatgtt tagataagaa tgttgcttat
    15181 gttgctggag gaggattctt ccctaatggt ggtagagaaa atactttcag acttaactat
    15241 tcaaatatgc cagaagaaaa aataatagaa ggtataaaaa atatagcagc tgtattaaaa
    15301 gaagctatgg gtgtagaagc ttaattaaaa ataataactt atatataata caagggatgg
    15361 ttgttgccat cccttgtttg tttgaaaagt atataattca ccttatacta aactttgtcc
    15421 aaaatttaaa ttgacagaaa taagaataaa tgataaaatg tgtaggtatg agattttagt
    15481 ctcatttctc tgctctaaga gtgtagagcc gttaagtcca aagggaggtg aattatagtg
    15541 agaaattatg aattagttta tgtggtaaag ccaaactctg atgaagaagt aagagaggct
    15601 atactaaaca aagttaagga agttgttgca actgacggag aaatagttaa agttgatact
    15661 tggggaacta aaaaattagc ttatccaata gctaagttta cagaaggttt ctatgtatta
    15721 gttaacttca aatcagcagt agacgttcct aaagagatag acagaaactt aaagataaat
    15781 gaaaacgtaa taagacacat gatagttgtt gcttaataac tggttttatt ataaaaaata
    15841 ggtggtgttt ttatgaatca agttgtatta gtgggaaggt tgactagaga ccctgaatta
    15901 agatacatcc caggtacagg aacaccagtg gcatccttta caatagccat tgatagagac
    15961 tatgtaaaaa aagatggatc taaagaaact gattttatac ctgttgaagt aatgggtaaa
    16021 tctgctgaat tttgtgctaa ctacataact aaaggtagat tagtagcact tcaaggatct
    16081 ataagagtag acaattatca gacacaatca ggtgaaaaga gaacttttac aaaagtaagt
    16141 actagatcag tacaagcttt ggatagtaag aataaatcgg aaaattcata tagagagagt
    16201 gctccagctt ttgagccaag ctttgaacct caaggtttag acccacaagg tttccaagct
    16261 atagatgatg acgatatacc attttaaaaa ggagggaaaa aagtcatgat gaataaaaaa
    16321 agacgtaaga aaaaaagagt ttgtcaattt tgtgctgaca aaaacgctaa aatagattac
    16381 aaaagtactc aaagattaca aaagtatata actgaaagag gaaagatatt accaagaaga
    16441 atatctggaa cttgtgctaa gcatcaaaga gaattaacag ttgctataaa aagagcgaga
    16501 aatatagcac ttttaccata tacattagac taaaaaaatg agccttgtgc tcatttttta
    16561 ttatatgtat ttatatatct aaatgaggcg agctataaag ctcgtttttt tgtttatata
    16621 cttaaatata ttattattta tgatataata ttatatatta aggagggttg taaacgtgaa
    16681 aatagataaa ggttctgaga taacgagaaa cataaagata atagaatgga tgaaaacaga
    16741 aatattaatg agtgtaagtg atttgtttaa tttattgttt aaaggggtaa aaccattaga
    16801 tgaagcaatt caagatactt tagcaaatat aataatgata acatatctct tagcaaagag
    16861 attaggaatt agttttagag atgtggatta taaagtcaag gaaaaaataa aaattggtat
    16921 agatgaggac catagcgttg aaagatggta tggggattta tctaatttaa aaaaacatat
    16981 ggataatagg gagtgatatt aaattgaata ataaaattag actatctaag gcaatgtcta
    17041 taatagtttt agctataata atcgctttag taatagccta tattccaatg ctaggaatat
    17101 ttagtatgtt agcagctgtt ccatatgtaa ttataggtgc tattactgat agaaagtatt
    17161 ctcttatatc tattttggtt acattttgcg tactgattct atttgcagat atttcttacg
    17221 cattaaatat atgtataatg tattctttac caggtatagt aataggaaag atgctaaaga
    17281 gaagctttga acaagaggac agtaataaat tcgaaccgat atatggtgga accataatat
    17341 ttgtactttc aatgcttgta tatttttttg tattaaagac atttttaaat gtgaatctat
    17401 tagatgaagt ttctaagatg atatcagaaa ttgtaaatat tcaaaaaaat agtttttctg
    17461 caactgaact taaagtgttt gataaaatga agccagatga gattgtaagt tattttacaa
    17521 atatgctacc aatgatgtta tttttacaag ggttgttatc agcgtttgtc acatattatt
    17581 tgagtgtatt ttttataaaa aggattacaa agatgaatat aagatttcct aagtttgctg
    17641 atttttattt accgggaaat gctatactca caactttctt actttattta ttagtcttat
    17701 ttattcaaat tattggttca aaattatata cagaattaat aatgactaat ttacagctag
    17761 tttttaattt aatgtttgta attcaaggta tagcagtttg catttatttc ttaaagaaat
    17821 ggataagaca gggaccaaat aaaattatgt ttttttcagg gataatttta tgtttatttg
    17881 gatttatggg aatatctttt gttggtatgg tagatagtat aattgacttt agaaaggtaa
    17941 gaagctataa atccacttag gaggatagaa atgagtaata aacaagcctt taaattaaat
    18001 atgccagaaa taaatttata tataattgtt attggtattt ctagtataat tttgctttat
    18061 tacaacttgt atgtagggtg tttatttttc tgtatttttg tatatatggt ttttcacaat
    18121 tggagaacta ctaatattag acgtcatgaa tggacagagt atattcaaaa tttatcttta
    18181 gatatagatg aaacaactaa aaaagccata ataaatcttc caataccatt atgtatttta
    18241 gaatttgatg gaaatatatc ctggtataat ggaaagtttt atgatatgat tggtcaaaaa
    18301 gatttacttg ataaaaatat agaggatata gttaaaaatc ttaatttaag aaaagtatta
    18361 aatgaaaata aagagatgta cacagaaata aactataaag aaaaagaata tacaataatt
    18421 tataatgtta taaaaaatga tcaagaaaaa aatcctaaat atttgatgat attatactgg
    18481 atagacaaga ctgaatattt aaaagtaaaa caaaattatg atgatgaaaa aaatgcaatg
    18541 atgctaatac aagtagatgg atatgatgaa gtattaaaaa gtgcagcaga agataagaga
    18601 gctttaatta atgttgaagt agagaaaata ctttctgctt tagaattaaa ttcaaatggt
    18661 gcattgagaa gaacttcaaa agacaaattc ttcttggtaa tgcataaaaa agagttaaaa
    18721 aaattggaag ctgagaagtt ttctatttta gatacaataa gacatattga ttatggaaat
    18781 aatcttcctg ttactataag tataggaatt ggtatagatg gagatacatt aaatgagaac
    18841 ctaaaacttg ctacgggtgc tctcgattta gcacttggta gaggtggtga ccaggctgtt
    18901 gtaaaaacaa aagataaatt tgtgttttat ggaggaaaat ctaaagctgt agaaaagaaa
    18961 accaaagtaa aatccagatt aataggtcat gctttaagag aggttataca acaaagtgac
    19021 caggtttata taatgggaca taaatatcct gatatggatg ctatgggagc agcagttggt
    19081 gtttatgata tatgtaaatc ctgtaacaaa actgcaaaca tagttttaca atctgtaaat
    19141 gaatctatag aaatatttat aaataaaata aatgaaaata attactataa aaagttgttt
    19201 attggaaaag aggaagctat tgacaactgc actaaaaata cattagttgt agtagttgat
    19261 actcatagac caaattatac tgaatgtgaa gaactgttaa agctatcaga gaaggtagta
    19321 gttatagacc atcatagaag aggtgtagag tttataaatg atgcagtgct tttattccat
    19381 gagatatatg tatcctcaac gtgtgaaatg gttacagagt tagttcagta tatggatgaa
    19441 gatgtaacaa taaataaact tacagctgaa gggcttttag caggtataag tctagatact
    19501 aaaaactttg cattcaaaac aggtgttaga acttttgaag ctgcttctta ccttagaaaa
    19561 gttggagcag atactataga ggtaaagaaa ttcttcaatt ctgatgtaaa agatttcata
    19621 ataaaagctg aaataattca aagtactaaa ataattaata atagaatatg tttagcatat
    19681 tcaagtactg agatagatag tataaatgtt ataatagctc aaactgctga tgagttatta
    19741 aatatcaaag aagtagaagc ttcctttgta ttaggtgaaa aagatgatac tatatttata
    19801 agtgcgaggt ctttaggaca aataaatgta catgtactta tggaaaaatt aggaggcggg
    19861 ggacacatag atatagctgg agcacagtta aaaaatgttt ctctaaaaga agcttataag
    19921 atggtaaata aaataataga ggagtatttg gaggaggaag aatagatgaa agttatatta
    19981 ttaaaagacg taaagggaac tggtaaaaag ggagaaatga aagaagtaag tgatggatat
    20041 gcaagaaatt tcttatttcc taaaaagatg gctgtgcaag cagatagtgt agcaataaaa
    20101 gaattaaatg aaaagaataa atctaaagaa ataaaagcac aaaaagagta tgaagaagca
    20161 gttttattag gtaagcaaat ggaagaaatt aatatagaaa tatattcaaa agcaggagaa
    20221 ggtggaagat tatttggttc aataacttct aaagaaattg ctgagcaatt aaagaaacaa
    20281 aaagacatag atgtagataa aagaaaaata ttattagatg aaccaataag atctttaggt
    20341 tcaactttcg tagaaataaa aatacatcaa aaagtaacta ctaaaataag agtggatgta
    20401 aaagaaaaac agtaataact agaggtgatt aagatggaag atatgacgag aattcctcct
    20461 catagtgtag aatcagaaca atcaatattg ggttctatac ttctagataa agatgctata
    20521 ataacggtta cagaaactat aaagcctgat gatttgtata aagaggctca caaaataata
    20581 tatgaatgta tgataacttt aagtaataaa ggtgagccta tagacttgat aacattaaca
    20641 gaagaactaa gaaaacaagg gcatcttaat gatattggag gaatcagtta tattacaagt
    20701 ctttctacta tagtaccgac gacttcaaat gtaaaatact atgctgatat agtaaaagaa
    20761 aagtctgtac ttagaaagct tataaaagct tctaatgaaa taataaatct aggatatagt
    20821 ggagcaacta agattgaaga tgttttagaa caagctgaaa agagtatatt tgatatatca
    20881 caggagaaaa ctagtgatga ttttaaatct ataaacttag ttttgatgga tgcctatgat
    20941 atgatagaaa aattatacac taacaagagt gatgtcactg gtataactac tggttttaaa
    21001 gatttaaaca aaaaaataaa tggtcttcaa aggactgatt taatattaat tgcagctaga
    21061 ccagcaatgg gtaaaactgc cttttcttta aacttggttc aaaatgcagc cttaaaaggt
    21121 gatgcctctg tagctgtatt tagtttagag atgtctaagg aacaattagt acaacgtatg
    21181 ttatcttcac aatctagtgt agaattaaaa aaaataaaga ctggaacact taatgataat
    21241 gattggccta gaattataga tgctatggca gtattatcag atgccaaaat acacatagat
    21301 gatactcctg gtataaaaat atcagaacta aggtcaaagt gtagaaagtt aaaaatagaa
    21361 aaaggactag atttagtgct tattgattat cttcaactta tggaaggtga aggtaataat
    21421 gaaagcagac aacaagagat atctaagata tcaaggtcac taaaaatatt agcaaaagaa
    21481 ttaaactgtc ctgtagtagc actatctcag ttatcacgtg caccagaaca aagagcagac
    21541 catagaccta tgttatctga tttaagagag tctggagcaa tagaacagga tgcagatata
    21601 gttatgttct tatacaggga cgaatattat catgctgatt ctgagagtaa aaatatagga
    21661 gaagtaataa tagcaaaaaa tagacacggt gaaactggtt ctgtggaact tgtttggctt
    21721 ggtgaagttc aaagatttgg ggataagttg agagacctat aatctaacga atatccacag
    21781 aacgtccttt ctttctacag gaaaaatgtg gatacatgtc actaaatatg tccaaacatt
    21841 gaaatttagc taaaaaacta tccacactat ccacagatgg cctgttgata agtgtgaata
    21901 agtagataaa gcaagctttt ggcttgcttt atctgtgtat aaatattaat tattaggagg
    21961 aataatatgg aaaaaataag cttaaaatat atatatccca atataattaa agtacttgat
    22021 gaaataaact tatttagagt aattgacaat aatttaagag aatctattgt tgtttatgct
    22081 aataatgtgg ataatcagta tcatataaac atgactaata caaactttgg aaacataatt
    22141 aacatatgta aattagaaaa acttttagat gtggataaat ttatggaaaa agttataaaa
    22201 tatgaaaaag aaataataga gaaagaagag ttttcaaaaa tagaagaata tatgttaaat
    22261 attggagaat actaaaaaag ttgaaaaaaa tacgaatgtt tgatagaata agtaagtaaa
    22321 gtgttcgaga gtatataaaa tatagaggtg aattacatga aaacagttgc aattgttggt
    22381 tctcaatggg gagatgaagg taaaggtaaa gttatagatt atcttgctac tcaagcagat
    22441 gtagtagtaa gagggcaagg tggaaataat gcaggacata cattagttgt agaaggtaaa
    22501 aaatacgcat tacatctaat tccatctgga gttttaaatc caaatacagt gaatataatc
    22561 ggaaatggaa tagtgtttga ccctaaagga tttttagaag aattggaaat gtttaaaacg
    22621 gataatataa gtacagaaaa tataaaaata agtgatagag cacatgtcat attcccatat
    22681 cataaagaac tagatgcatt gtcagaagaa gccagaggcg atttaaagat aggtacaaca
    22741 aaaaaaggta ttggaccttg ttatatggat aaaacagaga gatctggtat tagaatatgt
    22801 gacttaatgg ataaagataa atttgctata aaattaaaag ctcaaataga tgctaaaaat
    22861 gaaatagtta aaaatatata tggaaaagaa gaactatttg attttgaaac tatatataac
    22921 gaatatctag gatatgcaga acaaataaga aaatatgtgg cagatacgtc tgttattgta
    22981 tatgatgcag taagagcagg taaaaaagta ttgtttgaag gagcacaagg gactttatta
    23041 gatttagatt taggaactta tccatttgtt acatcttctc atccaacatc tggtggattt
    23101 gcaattggtg caggtatagg accaaatatg ataaaagacg ttgttggtat agtaaaagca
    23161 tatactacaa gagttggaga aggaccgttt gttacagagc aaataaatga aactggagat
    23221 aagataagag aacaaggaca tgaatttggg gtaactactg gtagaccaag aagatgtggg
    23281 tggtttgatg cagttatagt taagtatgca gctagagtta atggattgac tagtatttca
    23341 tttatgttac ttgatgtatt aactggattt gataagataa aagtttgtac gtcttataaa
    23401 atgggcgata agataataac ggatttccca gcttcattag atgatttagc aaaatgtgaa
    23461 cctgtatatg aagaattaga tggatggaat gaagatataa ctcaaataga taactttgat
    23521 gaccttccag agaatgcaaa gaaatatgta gcaaaaatag aagagctagt tggagtaagt
    23581 gtagatatgg tttctgttgg tccaaataga gctcaaacta taataagaag aaatatattt
    23641 gcttaatatt ttatcaatta aataatttaa aatagttgaa gaagagtatc tcaagataat
    23701 gtttatagtt attagagatg ctcttttata tttgacctac attgattttt tattaagtta
    23761 aagctactta tacatattct aactgaaatt aaagttgaat ttatgtagat acatataggt
    23821 tttttaaaaa gaattttgtt gaaaataaat ggctaaagat gattttaatt atattctaaa
    23881 aaaagtaaaa aaaactattg actaaaaatt atatagacgc tataatataa cttgtgagtg
    23941 aaagaaacga cgaaaaaagt cgaaaaaatg tggtctatta gctcagtcgg tagagcacct
    24001 gacttttaat cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa tatggctcgt
    24061 tggtcaagag gttaagacac cgccctttca cggcggtaac aggggttcaa ttcccctacg
    24121 agtcaccagt atgggactat agctcagctg ggagagcacc tgccttacaa gcagggggtc
    24181 acaggttcga gccctgttag tcccaccaaa ttttgaatta gagataaata ctagattata
    24241 tgctggtgtg gctcaacggt agagcagctg acttgtaatc agcaggttgt aggttcgatt
    24301 cctatcacca gctccacaaa gtacataaat tcaaattaat ataattttct atgttacagg
    24361 atacttttaa gtatcttttt ttaatttcat ttttattgta ttaaaaatga aattaaacat
    24421 acaacaaaat tttgaattac ttgcaatgtg atagttttaa atataaaatt tagaatcatt
    24481 ctagcatagt attatgtaat atagcaaatt taatttatag tatcaataaa tatagactac
    24541 taagaatgta aatgatttgt tagattaaga tacattagat atgtaaatag aaaaataaaa
    24601 agtatgtttt aatttatgtg atataagttt taaacagaga agcaagtttg ctcttacctc
    24661 tctatttaaa aataaaaaaa tgttttttaa gggctatttt atttaaatct ttcccatctc
    24721 aaatcatctc cagtaaattc taaaatccta aacttatcta atattctaga gagagttctt
    24781 tgagtgtaag tatttcctat ttgagatggt gttaagttgg ttgaaataat gattttttta
    24841 ccagcaacaa gtcttgtatt tacaatattg aagatttctc cactagtaaa agaattatta
    24901 agttcagttc caaggtcatc tattattaat aaatcacaat caaacaagtt tttataatta
    24961 tcttcactta tttgattatt tgtgtctctt ctaaatttat aatcttcaag aatatctaaa
    25021 attctaaaag aagtttgata gattacaaca ttgcctttat ctaacaattc ctttgcaata
    25081 caattgcaca tataggtctt tcctagtcca gtagaaccat aaaacagtag attttcacta
    25141 ttatccttct taaaatcatg aacaaaattt tcgcagatac ttaaattatt tagcatattt
    25201 tctctaggag atatttcacc atcagagcct ttcttaggag aaaatatatt caagttaaaa
    25261 ttcgaaaaat tttcctgact taaaatccta tctaaattag acattttata agcttcattt
    25321 acaatttctt gtttgaggca agaacatttt tctccattag gtaaaaatcc tttatcttta
    25381 caagaaatac attgaaactt tatttccaag taatcaagag gtatgttact ttcagctaaa
    25441 agtctttctt tctttacttt taaagactct atattctctt tagtcttctt tacaatttca
    25501 tctttagatt tgggatttaa aagtactatc ttagctaaac tgagacctat tttagaaatt
    25561 tcatcatcta tagattttat ctccgggatt ctattatata cctcattttt tctatgttct
    25621 aataagagtt cattatcatc tcttctctta gcatatttag caagtatttt tcttatttta
    25681 tcctcattca tttaatcacc tacttaaact tttctttttg acttttttta ataatctcat
    25741 cgagctcatc agaggtatac tgagtgaatg tttcgttgaa attatgaaac ttagtttttt
    25801 tgtaagtatt gttgttttga gtagtattaa tctgcttctt attaatattc tccttaacta
    25861 tacgttcttc ctctttttgc tttaaatcat ttaaattttt tatatttttt tcattccaat
    25921 ttttaattat accatttata taagatatac ttgggtttga aatattctta gacttagaac
    25981 atgcatttat tatcaaatct atatccatat taaatttatc aaaccaggtg tccataagtt
    26041 ctttttctga ttttgatggt tgtcttgaaa atcccaattc attgaaaatc gttttatata
    26101 aaatatatcg ttcacttctt accaaaaagc tttcttcaac atcttttggt gtatataaat
    26161 ttgaatcata ccaatttcta ataatgcctt caatgtattt aacgggcttt gatgtaccag
    26221 ttttatcttt gacgtattca tatgcgcaaa gtatcatatc ggggctcata ttgtatttat
    26281 tcattatatc cataatactc atcttttcac ttgggtcgag atatcgccca actattttat
    26341 ttatagaatt aaacattttt ctaatacttg gattttcaga agtagataca actttatctg
    26401 tattagattt tatagatgaa ttattgctta atatattctc aatatagaaa ttttttaaat
    26461 ctaaaaattc tattgaataa ttaaaattat catattctcc attgtcatgt atttttataa
    26521 tcttcttttc ttcccagaat ttccaagcag ataacacatc agacaatggt atatttaggt
    26581 ttttggctat tgaattattg tcgaacttag gattagaagt aatatcacat gcttgtctat
    26641 atcctaaaag ataaactttt acgtataacc cgtccgccat tggcataaat atatctataa
    26701 agatattggg aatagtgatt tcacctaaat ctatttcatt gctttcttta aaaaacattt
    26761 tatcacctca gattaaatta tatcattttt tatattatat ttgtctgtaa acaaataaaa
    26821 ggtcacaaga ttagcatgta aatattttct aaaagtattc attttgaaaa ggaggagata
    26881 ttttggttat aaaattcaac caaaaagtaa gaagaacatt atcagtagcg attctattta
    26941 tggtattaat aataggttgc tttatagaaa aggatgacag tttagaaaca tacaaagaaa
    27001 aaactaattc aaaaataaat cagtataata tggatgttat atttgatgat gagactaaga
    27061 ggttaatgtg caatcagaat gttgactaca taaataatac aaaatcaaac atagataaaa
    27121 tatatttcca tatatatccg aatgcttttt ccaagaaaga ttttgctcct tttgaaaaaa
    27181 gtgaaatgac aagggcttat cctaatggat ttaacgaagg atatatagat ataaagaata
    27241 tattaaataa taatagtaaa atggagtata aaattaaagg agacaaaaat gacatattag
    27301 aaatagaatt agggaaagag ttaaaaccaa atgaaagaat gtcattagat ataaaatata
    27361 acgtaaaaat accaaattca attggaagat ttggatatgg agaaaatact ataaatgtta
    27421 ctaattggtt tcctatagct tgtgtaaatg atgaaagggg atggaattta aagagttatg
    27481 aaactattgg cgacccattt tatagtgaga ctagcgattt tcatgtgaaa ctattgattc
    27541 caaataaata taaaattgct catacaggaa aacttattga tgataaacat gataataaga
    27601 aaatgctata tgaaatagaa gctgaaaacg ttagagattt tgcatttata ctaagtagca
    27661 aatttgatgt aaacaaagta gattcaaagg gagtagctat caatacatat aatttaaata
    27721 aaaagctttc taaaaaagca actgaagtgg caaaagattc aataaatata tttagtgaat
    27781 tatttggaaa gtatccttac aaagaatttt ctgttgtagc aagtgacttt tttatagggg
    27841 ggatggaata ccctatgctt gttatgatag accaaagttt atataatgaa aaaaatgaat
    27901 ttttactaga atatgttatt gcccatgaga cagcccatca atggtggtat tctgctgttg
    27961 gaaatgatga aataagtgaa ccctggttag atgaagcact tactgaatat tctacaatag
    28021 tttattttga acaaaagtat ggtaaagata tggcaaacaa gcttataaaa actatggaga
    28081 tacagacaaa aagttatttg agcgaaaata tatttaagcc agcaaatgag tataaaaatt
    28141 ctactgaata cagcttgaat gtttatacaa aaggcgcaat agcatttaat gaaataagaa
    28201 aagaagttgg agataaagta ttctttgaga ctttaaatga gtactataat aaatataagc
    28261 ataaaaatgc taatggaaga gcttttgtgg aactatggaa tagtaaaggt gtagatataa
    28321 ataagattat aagtgaatgt aagtaaacag tgaatgtaag taaattttgt gcagagtatt
    28381 aaattttatt taatactctg tttttttggt ataatataat ttataaggca gaaaaagagg
    28441 tgaaggaatg ctaaagtatt gttcagtagg gagtggtagt agtggtaatt gccactatat
    28501 agggtataaa aatactaata tactagtaga tgcaggatta agtggcaaga gaataactac
    28561 aggtcttaaa gacattggtg tagatgcaga taaattaaaa ggaatattta taacacatga
    28621 gcatgttgac catataaaag gagcaggtat attatcaaga aagtttgata taccagtatt
    28681 tgctaatata aaaacatggt gttctatgaa ggacaaatta ggtgacatca aagataaaaa
    28741 tatgaaagta tttgaaaatg ataaaaccta ctcattaggt gatatcatag taagaccttt
    28801 ttcaatagaa catgatgcgt ctgacccagt tggatataat ttttatacag agagtgatga
    28861 aaaaatgtct atagctactg atataggatg tataactcaa aatataaaaa aacatctata
    28921 taaatctaaa ttagtagtat tggaatcaaa ttatgaccct aatatgctta tgatgggttc
    28981 atatacatat gctttaaaga aaagagtaat gtcaagtact ggtcatttat ccaatgaaga
    29041 tgcagctaat ttctgcgttg aacttattaa tgaaggtaca gaatctatat tattagcaca
    29101 tttaagtaaa gaaaataatt ttcctgagtt agcgtatgag acttcaaaag gagtattagc
    29161 atcaaatgat atagtagttg gacaagatgt aaaactagat gtactatcac gaaatgatgt
    29221 ttctaatgtt tatgagatga agtagaggtg aataactttg ctaaagagaa attttgaaaa
    29281 tgttgaggat aataaaattt tatactctat aaagcagggt atgattttag cgatacctgc
    29341 tattatgact ggttcaactg ctcttgtaat tttgaatctt ccaataaagg cttatcaaga
    29401 atatttatca ggcttgttta atggagaggt tacaaatatt cttaacttta taaatgattc
    29461 tactttagga attatttcat taataatatt attaacaata agctatagtt atggaaaaat
    29521 atatgggagc aagtatacag tacttgttcc tatagttgca atgtgctctt ttttagtatt
    29581 ttcacatggt aatgagctta attcttatat agaaatattt aaaacaaagt ggttatttac
    29641 atctataatt gtttctatga catcttctgt attgtttgta aaacttacag aatcttttgt
    29701 tacaagtgta aagtttcata cagaaggggc tgatgcagac tttaatatgg tagtttcggc
    29761 tatagttcct ttcataatag tagtattttt gttttctatt tttagagtga taatgataag
    29821 tctaattggc agttcaaact ttcaagatat attttataat ttattttcaa atgtttttaa
    29881 taaaatggga acaaacctta tgagtgctct gttgtttatt tttttaatgc actttatgtg
    29941 gttttttggg attcatggta gtaatgtatt agataccgtc gcaaaaaatt tatttgaaaa
    30001 tagtatggca attaacataa atttagttaa taataatcaa ttaccaacag aaatttttac
    30061 taagaccttt tttgatacaa tggttttatt gggaggatgt ggctctttac tatgtttagt
    30121 aattgcaatt tttcttagtg aaaaacgtat aaatgtaaga aagttagcta aaattgcttc
    30181 aataccagct ctttttaata tcaatgagat gctagttttc gggataccaa ttgtttttaa
    30241 ctatattatg tttgtacctt ttgtaattac tcctataatc ttaaccatta caagttatgt
    30301 ggctatgtct acaggtattg taccatgtac agtttcagct gtagaatgga cctcacctat
    30361 atttttaagt ggatatatgt ctacaggttc aataagaggt agtttgttgc aagtatttaa
    30421 tttatgtgtt ggtgttatga tttatattcc gtttataaaa atgtcgcaaa atagatatac
    30481 atatatgttt aaaaataata tagaaaattt aacaaactta ttaaaaaggt atgaattaac
    30541 aggtgaacag cctaatcttc taaaccataa tggtaaaata ggttcaattt caaaaatgtt
    30601 ggcatcagac ttgaagtttg ctatgaaaag aggagaaata gagctttttt atcaaccaca
    30661 agtcaattat aatggaaatg ttgtgggagc agagggatta cttagatgga aacattcaat
    30721 aggagggttc atttatccac cactagttat aattttggct aaagaagaag ggttcttaga
    30781 tgagcttgga gagtatatta ttaataaagc atgtattgat ttaaagaaga gtgaaaagct
    30841 attgagaaat cctgttaagt tctctgtaaa tatatctcca gaccaacttg atgaccctaa
    30901 gttaccagag caaataaaaa atatcattag tagtaatgat ataaatccta atatgttagg
    30961 tatagaaata acagagcaaa ttgcattatc aggttcacaa ataattattg aacgtatcaa
    31021 tgcgatacat aacttgggaa taaagttaat tatggatgat tttgggatgg ggcatagctc
    31081 tcttatatat cttcaaaata ataactttga tatagttaaa ttggatggtt cattagttaa
    31141 agagatactt acaaataaaa gaagtagtga aattataatg actattgtaa atctttcaaa
    31201 aaatttagat tttgatatag ttgttgagta tgttgaaaat atagaccaaa gggataagtt
    31261 atatggttta ggatgtgaaa tatatcaagg atattattat agcaaagcaa taccttttga
    31321 agagtttgta gaatatgcaa atcaagaaag agagatataa ttttataaga gcttgtaaat
    31381 tctagtaatg catagatttg taataaaatt aagtttagga gaaatatatg aatataagta
    31441 tagtagtagt aggtaaaatt aaagaaaaat atttaaaatt aggtattgat gagttcaaga
    31501 aaaggctttc taagtattgt aagttagaaa ttatagagtt ggatgatgaa aaagctccag
    31561 agaatttgag tgttaaggaa atggaaatta taaaagacaa agaaggaaag aaaattttag
    31621 ggaagataaa gcataatagt tatgttatag ccttggctat tgatgggaag ggtttatcct
    31681 ctgaagaact tgctaagact atgagtgatt tagcagttag aggtaatagt agtctctgtt
    31741 ttataatagg aggttctcta gggctttctg atgaggtttt aggaagggct gattataggt
    31801 tatctttttc taggatgact tttcctcatc agatgatgag attgatattg ttagaacaga
    31861 tttatagggc ttataggata aataatggag aaccgtatca taaataaatg atacggtttt
    31921 tgttagtttg caacttaata atattaaaat aacaaaaaag tattgaaagt atattgcatt
    31981 taaggtagaa ttaaataaaa tattataaca ttataatgtt tatatggttg ttaataaaaa
    32041 tttggaggaa aatatatgat aaaaataatg ttggcctgtt cagcaggaat gtctacaagt
    32101 ttattagtta ctaaaatgga aagtgcagct aaagaaaatg gtatagagtc gcaaatttgg
    32161 gcaataccag aatcaactat acaaaatgaa atagaaaaat gtgatgtatt attactagga
    32221 ccacaagtta gatatgtatt acctaaggct caagaaattg ctaagccata taatatacca
    32281 gtagaagtta taaatatgat gcactatgga acagttaatg gagaagctgt tttaaataga
    32341 gcgatagaac ttaacaacac taagtaatat aaattataat gtatttttaa tatataaatt
    32401 gggagaatca aaggatggat gaaaagatta tagaaatatc ttttaatata ataggttatg
    32461 caggtgaagg taaaggatta gcttttgaag caataaaaga ggctaagaat ggaaatatag
    32521 aaaaagcaaa agaattatta aaagagtcta gggaagttat aaataaagca catagatatc
    32581 aaacagaatt aatacaaaac gaagctagtg gaaacaggac tgaaataagt gtcatactta
    32641 tacatgcaca ggaccaccta atgaatggca tgaattttta acagttagct gaggaaatcg
    32701 ttgatttaca tttgaaatta caagataaat aaaaattaaa gagaataaat taagagagtt
    32761 cttaaaagat acattatgtg tagcacacta attgttagat tattgattca atatttagtg
    32821 agtaatacat taggattatt ttgaggactc tttttatatt tagtttaaag ttaataaata
    32881 tttattatta ataatataag attatatgtt ataatattat aacatataat ttaagaggtg
    32941 aatcatgtta agaaatcaac cattatatat acaagttgta gatatattaa agcaaaaaat
    33001 attaaataac gaatataaaa ttgatgaaaa tattccaaca gaaagggagt tagagcaaga
    33061 gtttggagtc agtaaaatta ctattagaaa agctattgag atattagaat cagaaggata
    33121 tgtagagaaa aaaagtggaa aagggacaaa agtaataagt aatagccttt ttaataagct
    33181 atcaaaagca gagtcttttt caagtatact tttatctaaa ggtcataagt tatttaagga
    33241 gactatttca attgaaaaaa taataaatga tacaaattca caagtattta gtatatttgg
    33301 agaaaaatgt agcaagatta ctagaatgta ttatttagat agtaagccat atatattttt
    33361 tactcattat attccttttg atgaaaatct aactgttgaa agttttaata atgaaaattc
    33421 tttatatatg tacttatata ggagaaatat aaatataact agatttgaag atgaattttt
    33481 tatagaagaa tcagaactag aggtatcaaa ggctttaaat ctagctaaag acatattatt
    33541 aggaagaaaa agaaagacct atgatgagaa tgataatata gttgagctat catttgcaag
    33601 atataataca gaagtacaaa actatgtaat taaatacgaa atataataag aatcctgagt
    33661 ttattgaaat gataatatgc tcaggatttt tgtttagttg taaaaataaa aaatattata
    33721 acattataat gtttacaacc aaacattata atgttataat atttttaata aacaagtgat
    33781 attatcaatt acataaaaat tagtttatgt tatgaaatcg ttattgcatt ataaataaag
    33841 gataatatca ataatacagg gggataacta tggaaaaatt cgcaagtttt ttagagcgca
    33901 agattatgcc tgtagcagcg aagataagta atcagagaca tatgagagct gtaagagcag
    33961 gtataatagc aacattacca ctaacaatag tggggtcatt tttcacaata ttattaaaca
    34021 tacctattga tgcttatatg gattttatag ctccatttaa agcaatatta gatataccat
    34081 ttagatatac tgtaggattt ttatctctat atgctacttt tgggatagca tcatcacttg
    34141 ctaaaagcta cgatttagat acaacaggtg ttggtatgct tgcagttatg gcatttttag
    34201 tttctacaat agttccaact caagtagttg aaccagtagg tggagttata gaagctggta
    34261 gatggatgga tatggcaaaa cttaattcgc catcattatt tggagctatc gtaacatcat
    34321 taattacagt tgaaatatat cgttttatga aggaaaagaa tattacaatt aagatgccag
    34381 caggagttcc agtagaggtt tcaaattcat ttactgcatt attaccgaca gcagtaattt
    34441 taatgttatt ctgggtaata agacatattt taggatttga tataagtgtt gcactaagcg
    34501 ctatgttatc acctttaaaa ggattcttat caggaaatag tttaggtggt gggttactga
    34561 cagtattcct aataacttta ttctgggtac ttggaataca tggtccagct ataatggggc
    34621 cagtaataag acctatatgg gacatgtcta tagcagaaaa tataactgca ttcacagaag
    34681 gagtatcagc tcatgcaatg ccaaacatat ttacagaaca atttttacag tggtatgtat
    34741 ggataggggg agcaggggct actcttgcat tagttgtaat gtttatgttt tctaagtcac
    34801 aatatctaaa aagtttaggt agattaggat ttttaccagg gttattcaac ataaatgaac
    34861 ctataatatt tggagcacca atagttatga atccaatact agggatacca tttataattg
    34921 gacctataat aatgacaatt ctatcatatg ttcttactat tacaggggtt atacctatga
    34981 tggtagcaag attaccattt gcaatgccag caccaatagc agcagtaatg agtactaact
    35041 ggagtatact agcaggtatc ttagtattaa tcaactttgt gatatcattt gctgtttatt
    35101 atccattctt caaagttttt gaaaaacaac aattacaaag agaacaagaa gaaatgagtg
    35161 aaaatttata aaaatacata gatatagagg ttttgtacta tataaaatat agaaaaaact
    35221 tataagttat taagggtaaa ggtaggagga atgacctttg cctttaattt tatatatttt
    35281 aaataatgta atttgaggag gagaaagatg gaaagaagat taggaatatc agtatatcca
    35341 gagcatagta acatggaaga tgataaggca tatataaaaa tggcttcaaa atacggattt
    35401 aaaagaatat ttatgtgcat gctttcaatt acaagaccta aggaagaagt taaaaaacat
    35461 tttaaagaaa ttataaccta tgcaagagaa ttaggatttg aagtaatcct tgatatagct
    35521 ccaaacatat ttgatatatt acaaatatct tatgatgatt taacattctt tagtgagtta
    35581 aatgctagtg gaataaggct tgactatgga tatgatggaa gtaaagaagc tatgatttct
    35641 ttcaatccat atgatttaaa agtggaacta aatatgagta atgatgtagc atacttggat
    35701 aatataatta cttacaagcc aaatgtaaat aatatatatg gttgtcacaa tttttatcct
    35761 caaaggggta caggcttgcc atatgatttt tttgtaagat gttcaaatag atttaaaaag
    35821 tatggaataa gaactgctgc ttttgttaat tcaaaatatg caacaatcgg accttggaat
    35881 ataaatgatg gattgtgtac gcttgaaatg catagaaact tagatattgc tacagcaact
    35941 aagcacttat tcgcaacagg attgattgat gatgtaattg tagggaatgc atatgcaagt
    36001 gaagatgaat taaaaagaat gtctgaagta aataaatatc aagttgtatt taatgtagta
    36061 actgaggaaa aaacttctga agtagaaaag acaataatgg caaatgagca acactttaga
    36121 agaggggata taaccaacca agtagcaagg tctacagaag taagaaaaaa atatgctaaa
    36181 gaaaatttcc ttaagcatga taatacgacc atctttaaaa gaggagatgt agtgattggt
    36241 aatgatgact ttggaaagta taaaggggaa ttacagttga tacttcaaga ttgtgcagat
    36301 gaaagaaaaa ataaggtagg tagaataaag gaagacgaaa taatgctttt agactttatt
    36361 gaaccttgga gtaaatttaa acttatttta gagggagagt aaagtgaaga tagacatatt
    36421 aataaaaggt ggaaaaacag tagataaaag tataattgat atagctatat tagacgataa
    36481 aataatagaa gtaaaaaatc agatagatga ttctaagtat caggcaaatc aaattataaa
    36541 cctagatgga gaaaaattta ttagtgctgg ttggatagat atacatacgc actgctacga
    36601 aagactagac ctttataagg attatccaga tgaggttgga ataaaaagcg gtgttactac
    36661 tgtagtagat gcaggaacaa caggagcttt agatataggt aaattttatg aagatactaa
    36721 aaaatataaa acaaatgtat atgctttaat aaatattgct aagcagggaa taacatctca
    36781 agatgaactt tcaagtatga tgaatattga tgaatatgaa cttaaaagag cagttaaaaa
    36841 atacaaagat tttgttgttg gaatcaaagc tagaatgagt aaaagtgttg taatttcaaa
    36901 tgatgttgaa cctttaaaag tagctaaaag aataaaaaat gaacttaatc tacctatgat
    36961 ggttcacttt gggagttctc ctccaacgat agaggatata tttgactata tggaaaaagg
    37021 agatatatta acccatatat ataatggaaa gccaaatgga atacttagag gcaatgaggt
    37081 taaaaaagaa atcatagaag ctagagaaag aggtataatc cttgatgtag gtcatggaac
    37141 cgaaagtttt agtatggata ttgcaatgaa atctaaggat gcaggaattt ttccagacac
    37201 tataagtaca gatatatata ttaaaaatag aataaatggt ccagtgtata atctatctac
    37261 aacaatggaa aagtttatat atatgggcta ttcacttgaa gatataatag ataaagttac
    37321 taaaaatgca gcagatgcaa taagtttaaa aaataaaggt ttaataaaag aaggatatga
    37381 tgctgattta actatatttg atgtggttaa tgaagaaaaa gaacttcaag attctttgaa
    37441 taagtcagta atcacaagta cgtctataaa gccaagagca gtagtagtca atggagaata
    37501 tttaaatata ggtaagtcta taggagagaa tgagtagaag ggagaaacta ttgtatgagt
    37561 agtatatttg aaaaatactc tgtaagacgt gttataaatg cttcaggcaa aatgactata
    37621 ctaggagtat caacagtaga taaggaagtt attgaagcaa taggtgaagg gtgtaaaaat
    37681 ttctttataa tagaagaact tataaataaa acaggtgaat atatagcaaa tttattaggg
    37741 tgtgaaagtg cactaatagt ttctagtgca tctgctggga ttgcacagag tgtgggagca
    37801 atgatagcaa aagatgatat gagtctagtt tacaatgtaa ataatcctaa gaaacaagtt
    37861 aaaagggaaa taataattcc aaagggacat aatgttgatt atggagttcc agtagaagta
    37921 atgataagac ttggaggagg agaagttgtt gaggccggtt atgctaatat gtgtaacaag
    37981 gaacatataa taagagagat aaatgaaaat acagcagcaa ttatgtatat taaatctcat
    38041 cattgtgttc aaaagagtat gctaagtgta ttagatgcag tacaagttgc taaagaatat
    38101 aatttacctt tgatagtaga tgcagcagca gaagaagatt taaatgtata ttatgaattg
    38161 ggagcagacc tagtgatata cagtggtgct aaggctatag agggtcctag ctcagggtta
    38221 gtaataggta agaaaaaata catagataat gtaaagcttc aatcaaaggg tataggaaga
    38281 gctatgaaaa taggaaaaga aaatattgta ggtctaacat gtgcaattga gagatatata
    38341 agtaatgata aagtaacttt aaaagaaatg gaggacaagc taaatccatt tatagacaag
    38401 ataaatacta taaagggagt gagtgctagt ataactaggg attcagcagg tagagaaata
    38461 cttcgtggag aaattgactt taatgaagat ataataaata agtcaacaca acaaattata
    38521 aatgaactta gaagtggaga gatagctata tacactagag actataaggc taatgaaggc
    38581 aagattgaga ttgatataag aagtgtaact ggaagtgatt tagatactat ttataatagg
    38641 ataaaaaata tagtagaaag ttagagtgta gagtaagtga cattataatg atttaataaa
    38701 ttaaatttta ccaatggaga ggataaatat gaattgtata ccacaatatt ataaaaatag
    38761 agtttgttta aatgttcttg caggaagcat aaaaaatgct aaagaagtat atgatgcttg
    38821 tgacgggcat gtattgatag gagtactatc aaagaattac agcactgtag aagtagctat
    38881 tgaagatatg aaaaaatact caaaggaaat agataatgca atatctgtgg ggcttggagc
    38941 tggagaccca aatcagtcaa atatggtatc tcagatttct aaagaacttc agccacaaca
    39001 tataaatcaa gtgtttacag gtgtggctac gtctagagca ttgcttggtc aaaatgatag
    39061 tataataaat ggattagttt ctccaactgg taaaccagga tttgtaaaaa tatccacagg
    39121 tccattaagt tcaaaagaaa aagatggtat agttcctata gaaacagcaa tagctatgat
    39181 gaaagatatg ggaggaagct caattaagtt ttttccaatg ggagggctta aaacaaagga
    39241 tgaatatata gaagttgcaa aagcttgtgc aaagcataat ttttatttag aaccaacagg
    39301 aggtatagac ttagataact tcaaagagat agttcaaatt gcacttgatg ctggtgtaga
    39361 aaaagttata cctcatgttt atacatcaat aattgataaa gaaacaggtg aaacgaaagt
    39421 ggaagatata ggagtacttt ataaaataat gaaagaatta ttgggataga tttatgaata
    39481 tattagcttt tggtgaaata atgatgagac tttcagtacc tgactacaag ttcttaacac
    39541 aaaccaatga actaaattat ataattacag gcacgggatt aaatatatta tctggactta
    39601 aaaactttgg atacaatacc tatatgttga caaagttgcc taataataat gtaggtaaag
    39661 catcaagtgc aaatataaga aagcttggag taaaagatga ctttatcacg tatggaggga
    39721 atcatatagg agtgtatttt cttgaaaatg ggtatggaga aagaccttct gaagtgactt
    39781 atttgaatag gttaaatagt tcattttgtg agagtaaatt acaagattat gatattgata
    39841 aatgtttaga agaaatggat atagtccata tttgtggtat tgctatgcaa cttacagatt
    39901 cagttaaaga gattgcttta gaactagcta gaaaatctta tgaaaagggg ataaaagtat
    39961 tctttgattt taattttaga caatcattaa atccagaaag gacttatgat gatttagtaa
    40021 atgattataa gaaaatacta ccatattgtc atgggctatt tgctagttat agagatataa
    40081 ataagatact taatatagat agtctaaata taaaatctag ttcagaattg attaaaaaag
    40141 agtatgatat agatattgta gctggaacta tgagaagatt tgatgatatg aataatagat
    40201 ttataaaggg atatatatca aatgatgatg gttattttga aagtagggag tataagttag
    40261 aaatatatga cagagttggt gctggggatg gatatgtggc aggaattata tattcatatt
    40321 tggaaaatat gaagagtgaa gatatagttg agtttggggt tgctagttcg gtgttagctc
    40381 atacaactta tggggataat tctttagtaa gcacaggtca tgtacttaga ctgatgaaca
    40441 atgaaaaaat agatatattg agataataca tttgtatata ataaaatttt tatctttttt
    40501 agacacattt ttgacttgct tatatttgtt aaacagagtt atttttaacg attataaata
    40561 ttaaattaga agttaaataa taaaatagta gaaaatagca ttaactatag aaaaaatggt
    40621 taatgctatt tttatcgtta atattgatta attttatatt atagaaatga acttttctaa
    40681 tgctggattt gtattttttt tattccatac aactacaact tcaattctat tttctacttc
    40741 ttttataggt actattgcaa tatcacgttc tatcattgta tctgtaacac aaaatgaagg
    40801 taaaatggat attcccatgt tagaagatac caatgattca atagtattaa gattatttga
    40861 acgttgtacc acattaggta caaaccctgc ttcacgatat ctattctgta tcatagattt
    40921 ttgtccatac tcatcccctg tttcctttac aaaaataaat ttatcatgtt gtaattcaat
    40981 taaatcgata gaatcctcat tgtaaagtgg atgaccagga ggtaaaagag ctaccaaaga
    41041 atatctttct acagttttat attcaaaatc ttttaatggt tgattttctg gattaataac
    41101 aaaagcgagg tccatagaat tattttttac taagtttaaa agttcggtta tttcattagt
    41161 acaaatatcc atagatatat tagggtatgt attagaaaat tcttttaaat attgttgaag
    41221 tttgtttttt tcatatccta ttaaggttcc aatgctcaaa gttccaaaaa aaccatgtgc
    41281 aaattgttgt gtttttgcaa tagcatcatt gatatttttt attatagtac gtgcctcaga
    41341 taaaaaaact tttccagctg gtgttagttc aacatgacgt ttacttcttg taaatagagt
    41401 tacatttatt tgttcttcta aagcttttat ttgctgtgta acagcagttt gagaaatata
    41461 aaatttttct gctgttttgg taaaacttag attttctgct acagatataa aatattctaa
    41521 ttgtttagta ttcaaataat cacccctatt taaacttgat tgtttgtaaa ccttatttat
    41581 ctataattaa agcttattat agtataagaa aatttacttt taaagaatat tagaaaatat
    41641 ttgtataaaa atatatgttt taagtatatc tttacattag attttataaa tttattaata
    41701 agaataactt atatattcca atgaaaacga tattggattt aagtaagttt gtctcataaa
    41761 atgaaagtat atcaattgat aatactttaa aataagaagg gagaaaaaat ggaagataaa
    41821 ttttatgcaa aaggcaacgg aaataacgga tatattaaaa atcttgaagt ttgttccttt
    41881 aataacttag atggaacttg tggaatgttt caaatggctc tgtacaaaag agatgaaaaa
    41941 tactatttat atggatgctg ttttggagga aataaaaaaa atggagtaat gattagcgat
    42001 attacagacc cttataatcc acaatttata aaacattttc aaatgttaga ccctaaagag
    42061 tatcctacaa caacaactcc caaaattcaa atagcagatg atttaatgat agtagcaatg
    42121 agttgtggaa gtggaccagg agcacttgtt gaccaagcta aattagcaaa tattaagtgt
    42181 gaagcaggaa ttagaatata cagtttaaaa gaagaccctt taaatcctaa gtttttagga
    42241 tattgggatt gtggcttaaa gcatgtaatg ggtgttcata gattcatgta caatggtgga
    42301 agatatgtac atttatcaag tgattgtgtt ggctttgaag gtctgattta tagggtcata
    42361 gatataataa atcctactaa tccagtggaa ataggtaaat ggtggagacc agaccaatat
    42421 gcagatggat atccaaatag aacttttgat gcaggagcac ctcattgccc agaatttatg
    42481 gataaaggat ggcttcatgg acctccattt gtaagagacg gaaaagcata ttgtggttat
    42541 ggaggagctg gtttagttgt attagatgtt gaagatttaa caagaccaag atgcttaggt
    42601 gaattgccat ttacgcctgc attttctagt agacttgcag gtgcaagaac tcatacagca
    42661 ttaccattgc caggaagaga tttagtcgtt gttcaaaatg agggagaaag attccagttc
    42721 tttaaaccag ataacattac agatgttcaa gctatgaata atatacatat ggttgatgtt
    42781 agtgacccaa caaaaccaac attaattgct caatttccat atcctgaagt tccaaaagat
    42841 ttcccttatc ctaactttaa tgttgcagga ttaggaaaac cagggccatt tggtccacat
    42901 aatcttcatg aaccaatgga taataagcca tggttagagc aaagaggaga tagagtatat
    42961 tgctgttatt tccatgcagg gctaagggtt tatgatgtat cagacccata ttatatcaaa
    43021 gagctagcat attttatacc accaaatcca aataaaacac cagaagaatc ttatttccca
    43081 ggattcccag gaccacgctt ggcagtaaca gaagatctta tcgttgatga tagaggctac
    43141 atcatcatag atgctttaga tgatggattc tatatattaa aaatgaaaga agattaaata
    43201 aatactaaag agggagagtt gaaaatgaaa atactaggta tttcttttgg aacaaaaaat
    43261 ggaaataatg acacaatgtg taaagttgca cttaaaggtg ccaaagaagc aggggctgaa
    43321 gtagaattta ttcaaatgtc atctattaac attaaacatt gtactggatg ttgtgcgtgt
    43381 gttaaaactt tattatctgg gaaaggtagt atgtgtgtat taaaagatga ttttgaatgg
    43441 ttgcttgaca aaatgaaaga tgcagatggt atagtagttt cagaccctat ttttgaagaa
    43501 ggggcatcag gattattcca tactataatg gaccgttttg gacccagagc agatagagga
    43561 aataacatta taggaacaaa agttgcagaa gttataggag gaaaaattcc agattcaaga
    43621 atgcttaatg ataaagtaat atcatttatg ggaataggag gttctgactg gggtacaaga
    43681 gttcaatgtg aacatgctat gctagctctc ataccaatgt ggaaagttat agataatgct
    43741 tggttcccat gggcaaagga attagtaatg gatgatacaa ggcttgttca agttcatcaa
    43801 attggtttaa atatagttga agcagcaaaa gattttgaaa aagcttcata tcaaggagaa
    43861 gaaggtgtat gtccacattg ccacaataga ttattctacc ttgagccagg tactaaaaag
    43921 gcaatttgtg ctctatgtgg tatagttgga gagttagata ctataagtgg aaaaactata
    43981 tttagttttc cagaagagca attaggtcat gctcatgata ctttaccagg taagtttata
    44041 catggtgatg atattaagaa aatggaaggt cattatgcag aagttcgtaa aacacaagaa
    44101 tttaaagaaa gaaaaacatc ttataatttt atagagccat taactaaaga aaagcaagct
    44161 ggagtttcat taggataata attaaaatac taagactctt ttttaaggga tgttagatgt
    44221 tataggaggc ttattgtatg aatgaaaagc caagttttaa acaaattgaa gttattttag
    44281 cagttatttt tttggtactt acatattaca aggttagatt aactccagca tttattgtta
    44341 tatttatacc actatattta agttattttg cagtatctat gattgtaaat caaatagcaa
    44401 ttaagctatt ggagataagg caagctaggg aagctactaa ttacaatata ggaaatggat
    44461 agaaagaaaa aagaaatatt aacactgtct atggggattg ctttactacc tcctttatgg
    44521 gcagtattag caccttatat aggaataaaa actggagctg tagcactaat ctgtgcagga
    44581 ttatatgtaa ctaatggtaa taaacaaaaa gatggattaa aaataatgtt tggtttttgg
    44641 tgtggagatt tatgggcagt gttagccatt ttaattatgg gatatatgaa tttcaatcaa
    44701 aacctagaat tatttttaac acttagcata ttaggttttt ttgcagttgt aattgcatcc
    44761 ttatttgaaa aaataatttt cctaccatca tggctttgtg gttgggctat aggtctaact
    44821 gttatgacag gtgaaaatat tattaattta caagatattt gtattcaaat tgctgtagct
    44881 atggcagtag gagtatggta cgtgggtgca ggtgtagatt tgtttacaat atatattttg
    44941 agtaaagata ggaaaaaggg aaattgaaag catgtgaata tactttgatt ataggagaat
    45001 ggctatgggt tttgaattta aaataatgag aagtttaata tatgtaggac ttgccaagga
    45061 agaatataga cctaagctaa tggactggtt atatcgtcac catattccag atagtattag
    45121 cacttttgga ccatattgta ctaaatatgc cttttatcaa gcatatccta caccaaatga
    45181 aggtgagcgt tttggtgcac gtaagatgca actaacagaa cattattggc ttgtagatga
    45241 acatatgcct gagatggcaa atagaattat gacagaatat atgcctatgg atgttctacg
    45301 ttggcaaggg tgtataccag atgtagaaaa taaaagggtt catgaaaatg tagaaagtgg
    45361 agatgcagga cgtgcagtag gtggagataa tggatgtcca ccatttatat ttgcctttgt
    45421 tccaataaac tgggaagaag actttagagg aaaaggacgt actgtacaag atggaccaaa
    45481 ctatcgttgg caatttatga ttaagtatcc agatggtatc tctaaagaag aaggagaaaa
    45541 atggttctat gatgaggtag tgccatactt tacaaactgt tgctatgtta atcgttttgt
    45601 cagtagtaaa ataatgatta attatggagc aactgctttt gaccgtgtat cagaactatg
    45661 gtttgaaggg gaagaagaat ggtataaagc tgtggttgaa gagacaaagt cgtttattaa
    45721 aaaaccagaa tgggcacaag aagaggagtt cccatattta aaaccacaat tcaatatcgc
    45781 atcagtattc ttaggtgata tagcaactat ggatgcatac tcacagtatc gtggatatat
    45841 accaatgaga taaaaattat agtttgtgat aagtagagga ggcatttact cttactggta
    45901 gaagaagact tattgtatcc ttaaaagtag gtgaatatat agttttatat ttaaatttat
    45961 ataaaactaa gactaataaa tatgaaacaa tagaattaga ataatttgat aatatttgtt
    46021 aaaagagcat ttagaatgtg tttaaagatt aagatattcc ttttatggta gcagatagtt
    46081 gaaataataa aactgttatc atgaaaggaa tatttttatg tataggaaat ttttaaaaat
    46141 aaaagttaaa tatgtttaag aacatcttaa agtgaaagaa tcattgttag aaatatgtag
    46201 acaatatagt ctttataacg tatcatatta acaagtttac acttggatta tgaaataaaa
    46261 agagtaattc caaaggttgt aaatactatg ctgactttga tacttcaaag tttaggtgat
    46321 tttgtaattg ttaatagaaa tacgtttaat gagcataaag aatttatcta ataataggag
    46381 aatataatgt atacaattgg acaagtatct aaatttttag gaatatctag agatacacta
    46441 aaattttatg aggacaaagg attagtaaat cctaagaaaa atgatgaaaa tggatataga
    46501 atatataatc aggttgatat ctatgatatt gcaacaataa acttttatag agaaattgat
    46561 atagaaatta aaaagattca agaaataaga aaaagtaaga gtataaacaa tttagaactc
    46621 ttactagaag agaaggaaca aatcatacta aaagagatag aatataaaaa gcttctctta
    46681 aaaaaggttc agttagtcaa agaagattgt gacaaaataa aaacatattt tggaaaatac
    46741 acaataaaag aaatgaagcc tttggaagta aaaggtaaaa taaaacatgt tactaactat
    46801 gaggaatatg aagccctaaa agagaataca aacaacttaa aaaaagcagt tacattaact
    46861 gatttaagaa gagtaataaa ttttaatgaa gaaggtataa tagaagaaaa atttataatt
    46921 gtaagggaag tagatgacta caacaaaatc atagaaggtg aagttctatc tcatcccaat
    46981 tgtatttata ctattattga agatggcaga tggtcaactg gaggagaaaa tattgataat
    47041 aaagtggaag acagattaag agaaatagca atagaaaatg aatgtgaatt acttggaata
    47101 gtctatgtta atatattgct tactacttat gaagaaggga ttgagcgtat atttttagag
    47161 atttatgcac ctataaaata atataaaagc attgacctgg gacttactca taggtttaaa
    47221 ctcatgatat aagcagttat aacttatata gcaattaaat attaatttga aatgaggatt
    47281 acttatgaga acagagaaat cattaaaaag gcctatagcc agttttatta ttctaacaaa
    47341 tataattttt ttaccacttt ttttattggt agtagtgact ataatgttaa aacttccaac
    47401 agtgatatct gacattgcgt tgtgtatatc agcgtggtca tccacttttg catttatgat
    47461 attatttaaa aaaatttatc cagataagaa atttttagta cacgtaaaag atagatttag
    47521 aaataaactt aaattttcaa cagtatctat aataattagt atacaagtcc taatagctgt
    47581 agtagttata tttatattag catctaaaaa tcatggaata atgcctaact ttacagtttc
    47641 atcatgggga gcatttatat atttattttt aaaaaattta tttgcaggat cattaggtga
    47701 ggaattaggt tggagaggat ttgttcaaaa tcatcttcag aaaagacatt ctcctttaaa
    47761 agcatccata atagtaggtt tttggtgggg gatgtggcat ttaccaatat ggtttacaac
    47821 aggattcaaa ggacttgatt taataaaata tattatattt tttatgattg caattatatc
    47881 aatcagtatt ataatgacaa ctttttataa tctaaataaa actttgataa tacctattgt
    47941 aatacatcaa ctatttaatt ttttaatagg gataattaat ggagatttaa tagagcttat
    48001 aaattactat gcaatgttat atttaatagt tgcaattgta ttggtagcta taaatccagg
    48061 aaatgcctta tataaaagat tttctaaaaa cacaacacaa atactttaat aatataagtg
    48121 agaatcatat cacaaatgag agaaataatt ctccttatta attaatttta attaataaat
    48181 attacaatag atttatctgg aatggaaggt atgcagagtg tatttcatta gatgaaacac
    48241 ttaaaagaat gaaatagata agtatcaact tattatatga gagaaacttt aactttcttg
    48301 gtttaaaata ttaaaattat aaggaacatt tattattaaa tactttattt atatgaatta
    48361 attgaagaaa aaggatatta aactacaagt taaaaaataa atttaatata ttagaattat
    48421 aaataaggca cattaatttt attttaaatt aatgtgcctt ttatttatgg taatttatgc
    48481 ataattagta tcgttttgat tctttaaata aatttttctt ggtttcttca cataaggaac
    48541 tttttttaat ttataaaatc tacagttata ttatagtatc tattttagag aagtttaatt
    48601 tttgtatcaa tctaaaacaa aagttatact taaatgaaat tgaaaacgat aaaattctca
    48661 aataaaataa gtattgtgga ggtgatgaaa gaatccaatg ataatatgaa gactaatctt
    48721 aataagaaag taggggttaa tggtagtgga caaagaaatt gtatcaattc tccagatttt
    48781 attaaatagc acattgatta caactaatgg attacaagag gagtctaatt catcaaagag
    48841 acaaattact tatagaataa ataaaatcaa tgacatgctt aaagtaaaaa atgtaccact
    48901 tatatgttta agagctgata aagatattat cgttagaaaa gaaacaaaaa aagcaattaa
    48961 agaaatttta gaaaaaaact attctaagaa tacttattat tttagtaaga cagaaagact
    49021 tttatatatg tatttaatgc tttttattaa catggaggat atttcaatta atcattttat
    49081 aaattctata aaagtaagta ggtctactgt aaatcttgat ttcaaggatt tgataccaaa
    49141 gcttgaaaag aagaacatta aagttaagaa caatcgtata aatggatatt acatagttgg
    49201 caatgagatg gaaattagga gagtgcttat taaaaatatt atagaaactt tgtctaatga
    49261 gaaaagttca agggtatttg atatttttat taaagaatat aatttagata gttttgaaga
    49321 ggctaaaaaa attatattaa agctaataaa aaaatatgaa attacttttg ttgaagatag
    49381 attaatagaa tttatatata tatttatctt tctaaaggca aggatgtatt caactagaat
    49441 agttactcag gaaaatgtag atattcctaa tatttcagtt atgagttcaa tgaaggagta
    49501 taaatttgct aaggaattat tagaagtata tgggagtgaa gataaaataa agcctcacaa
    49561 tataatgtat attagtgctt ggattttagg tatatcagta ggaaattatg aagaagatac
    49621 agaagataag gatgtaattt caaaaattgt aaataaaatg atgacaaagt ttggatattt
    49681 aagtggagtt tactatataa gccaagaaaa aatatttaaa caattatatt ctcattttag
    49741 acctgcctac tatagacttt tatttaaatt acctatctat aatccgattt gtgaaaaagt
    49801 aaaagaagaa tataaattag tttatagaat tgtaagtaat gcaatgaaag agttttgcgg
    49861 attatttgga gaagaaattc aggaagaaga gttagcatat cttacaatgc actttgcaac
    49921 acttttttca aataaaaaag aatttgatgg ttttagaaaa agaatagcat taatagtttg
    49981 ttcaaatggc gttggtagtt cagctatttt acatgcagaa cttacaaact tatttcctga
    50041 cttacacttt ataccaatgt cggaatcagt tggattagaa aatataagtg aacctgtaga
    50101 tattgtattt actactaatt ataacgcaga gggtttaaaa acagatgtac cagtaattaa
    50161 agtaagccct gttatgacca caaaagaaaa atataagatt acacgtgaag tgtatattca
    50221 attaggtgat atttttttaa gacaacctaa aatagatgag gttatggata taataaaaaa
    50281 atatgcaaat gtaacctctg aaagtattct ttcaagtgag ctattagctt attttacaca
    50341 gatagagaat tttacatcta aggaaggaga ggggcctatg ctaagtgaga ttactaatga
    50401 aacactagta aaaataaagg taaaagctaa gaattgggag gaagctatta gacagtcagc
    50461 ttctgtatta gttgaaaata acaaagtaac tgaagggtat gtagatgcta tgattaactc
    50521 agcaaaagca tctggaccat acatagtaat tacaaaacat gttgcacttc cacatgctag
    50581 accagaagct gggtcaaaag agattgcaat aggcattgct actttagata gtcctattga
    50641 gtttgggaat aacgaaaacg accctgtaaa atatgtattt tgtctaagtg caatagacaa
    50701 taatagccat ttacgtgcta tgtctgagct agttgaacta ttggaagaag atgagttttt
    50761 taaggtttta gatagtgcta aagaagctaa agaaattatt gattttataa aaacacacga
    50821 attataaagg aggatttaat tatgtataaa attttaatcg catgtagagc aggagttggc
    50881 tcaagtttaa tgttaaaaat aaagactcaa caaattatta aggaaaataa ctttccaatt
    50941 gaagtagagc atggttcatt ggattcacta aatggattca atggagatgc tgtagaaaca
    51001 ttaattgatg ttgctgaaga attaaaaaat aaaaatttga agtttgatat aattgggatt
    51061 cacaatattg ttgaccgtaa tgaaataaag acagcactag aaaaattcct tgcttcaaaa
    51121 gagggataaa tttaagatga taaggaggaa tatttatgga gtttattatt agtttgctga
    51181 gtacacctgc tgttttgctt ggcgtagttg catgtatagg attgattctt caaaagaaat
    51241 cagctgttga agtatttaca ggctcttcaa aaacacttat cggttttttg atatttggta
    51301 ttggtgcctc tgctatgaca ggagcacttc aaaatttcaa taaactgttc caacatggct
    51361 ttggaattac tggtgttata gcttcaccag aagcagctac agcacttgca caaggtacat
    51421 atggttttgc agtttcatgt acattaattc ttggattcat attaaattta gtatttgcac
    51481 gtattacaaa gtttaagaat attttcttta caacaggtca tagtttgttc ttttcttgtg
    51541 tattagtttt aattatgaaa gcacatggat ttgataatac aatcactatt ttaataggtg
    51601 gaactatctt agggtttatg tctgctgcac tacctcaatt ctgtcaacca tttatgcgtg
    51661 aattaacagg tggcgacgaa caagctattg gtcactttaa catgattggt tatggtttat
    51721 caggttatat tgggcgttta ttttcaaaac ataaagatga tacaacagag actatagaat
    51781 ttcctaagtg gttatcaata ttcagagact tcttaatggg tctatccata gttatgttaa
    51841 tcttatttta tattgcaaca ttaaaagctg gtcaagcatt tactcaagaa attgctggtt
    51901 caacacattg gttggttttt ccgcttatag aggcatttac atttgtggca ggtatgtcaa
    51961 tattgatgtc aggtgttcgt atgttcttag ctgaaataac tgcagcattt gtaatcattt
    52021 cagaaaagta tattccaggt tcacgtcctg cgctagatgt tccaaccgta tttccatatg
    52081 caccaaatgc agttattatt gggtttatat cagcatatgc tgctggtcta ttagcaattg
    52141 ctattatggc atcatttcct agtatatttc ctgtagtaat tattccagct gctcacatat
    52201 gtttcttctc aggaggtaca gctgcaatat ttggcaatac ttcaggagga tggagagggg
    52261 ctattgctgg gtcatttgtt gtggggttat tacttgcatt tttgccagtt atactatatc
    52321 cagtttatgg gactcttggt atagaaggag caactttccc taatattgat tacaacgtag
    52381 ttggttcgat attacataat attttacaat ttataaaacc tatttaaact aaattatata
    52441 aaagtatagt catagttact atgatttcaa ggaggataca tcttatggaa aatacattat
    52501 ctgaattttt agataatata tctaatgaat tgtcagaatt tattgcctca atagatgaga
    52561 catctataaa taaagcatgc gaattaattc ttgaagctga aaaaaatcac ggacgtgtac
    52621 atgtgactgg tattggcaaa ccaggacatg tatcgggata tatttcatca ttgctttcat
    52681 ctactggaac gtcagcatat atattgcatg gtacagaagc agtacatggg tcaagtggtc
    52741 aagtagttga aggagatgtt gttattgcaa tatcaaatag tggtgaaact caagagttaa
    52801 aagcaactct taagactcta aaagtaaatg gagcaaagat aattggtgta agtggtaatg
    52861 agagttcatt tttaaaaaat atttctgata tattcctgtt tgcaggtgtt aagcaggaag
    52921 gagattgtct caataaagct cctcgtgctt caattttagc tgaaacaata atattacaat
    52981 cattaagtgt tgtattacaa tatgctaaag gtcttaatac acagcagtat ttgaagtggc
    53041 atccagcagg aagtcttgga aagtctatta gagaggggat ataattttat gaaatataaa
    53101 gggattatat gtgcaatgat aacaccattt gatgaaaatc aaaatattaa ttcacaagca
    53161 acatgtcaat taattgatta tttgattgaa aagggaattt atggattatt tattttgggc
    53221 actaatggtg aatgtcatgt attaacagat gatgaaaaag ttgaatttgc aaagattgtt
    53281 attaatcata caaataatcg tgtacctgtt tttgttggaa cagggggtaa ttcaactcgt
    53341 gaagttgtag atttatctaa gagaatggaa gcgattggag caagtgcatt atctattatt
    53401 acaccatact ttgttacacc aacacaacag gaattaatct tacattacaa aactattgct
    53461 aattcagtta atttacctat tataatgtat aatatgccag gtaagactgg aattaatatt
    53521 gaaccagagt cagtacgtga attatcaaag ataaagaata ttgttggaat taaggatagt
    53581 agtggtaaac ttgacaacat gaaagcttat attgaagcaa cgaagaatga gaattttagt
    53641 gtgttttcag gctcagattc acttattctg gatagtctaa aagctggtag tcaaggagct
    53701 gttgcagcaa caacaaactt cctcacagag attggtatct ctatctatga aaactttatg
    53761 aaaggggatt tggaagaagc tcaaaaagct caaagtagta tagaagaact tcgacgtata
    53821 ctgaaacttg gaacaattcc atctgttatg aagaaaacta ttgtcttgaa tggaattaat
    53881 gttggaactg cacgacttcc tgtaactgaa ccaactgggg aagtacttga agaaattaaa
    53941 cgagtagttg aaaattatag gacaatactt aataaaagta gtgagaatag ataatataaa
    54001 tagggaaatt acactggtat ataattaata tatatattat aaaacatgac taaaattaat
    54061 gaataagtaa agtggtttga tgagtataga ggtgataata tgaaaaagat gaatataacc
    54121 aaaagaatgt cagaatgtgg ggctcttgca attgtaagag aagaaaatct taaccgtgca
    54181 tgtgaaattg ctgaaggatg tataaaaggt ggtataacag ttatagaaat gagttatacc
    54241 ctaaataatg ctggagaaat aattcaaggg cttaataaaa agtatggtga aacactttgt
    54301 gtgggagcag gaacagtttt ggatagtgaa acagctcgtc atgctatatt acatggagct
    54361 caatttatta ttgcaccaaa ttataatgaa ggtgtagcga agatttgcaa tagatatcag
    54421 attccttatg ctccaggatg tacatcattg actgaggcag tagacgcact aagtttagga
    54481 gcaactttta taaaagcttt cccaatatca gatttctatg gacctaaact agtaaaggta
    54541 tttaagacac caatacctga tatgccagtt ttagcaagtg gagggattaa tattgaaaac
    54601 ctacatatat ggttagaaaa tggagttgat gtatgtggat ttggtggttt attaactaaa
    54661 ggttctgttg aagatattgc taaaaatgct aaaaagattc gtgaaatcat taaaaatata
    54721 agaaaaattt aacatagggc tgtatcatag tatcttataa ttactatgat acagcctttt
    54781 taatattatt caaatttaaa acagtttaca tttctattat attacatata atttcagtaa
    54841 ttttatctta atttttctat ctggaaaaaa atgatttaaa acttaagtta tgatataatt
    54901 tcattaaaag aaggcgattt ttagtaaata tgagaaaata atgtgttttt aggggaaaag
    54961 ttaaatacag atttaagtga acaagaatcg ataattaaga tattaaatgg tgaatagggc
    55021 atcaaaaaaa ttactaaatt aaaagtttta gagggagaat cttagcaagt gtgtgtgaaa
    55081 attcaatttc aaacaaaaaa taattgtaaa tgaaatcaat ttatacacgt tgaagattca
    55141 cttttatcat tatattaaaa agcagagtga attaaaagtg agtctaaaga taggaggata
    55201 attaaaacaa tgaaagaaga cattaggtta catcgaatgt gtaaaaaaga acaagcaaca
    55261 aagaatgaag ttatatcaga ggatattcta aaatctatga gtattggggt cagtaaacat
    55321 tttttagatg attcatttac aataatatgg ggaaatacat cattctatca attattagga
    55381 tatacagaag aagaattttt agttaaattt tctagtttaa aagattatta tgaacagaat
    55441 ctttatcatt ttgaatctat gaaaaatcat tttttagagg cttatcatca tggcgaaaag
    55501 agtatagaat atcaaatttg catttcagca aataatggag accctgtttg ggtaataatg
    55561 actggtatat taattgaatc acaagataat gagaaatcaa tggtctatat attgtattca
    55621 aaaattaatg agttaaaaat aaagcaagaa gaactatctg atatggaaaa agagagtaca
    55681 gagaatttta aatggatgat gtcagagtat gctgggaatg tttatattag cgatatggat
    55741 acatatgaat tactatactt aaataagcac tcatgcaata ctttacaggc atcagcaaat
    55801 gaacttattg gaagaaaatg ttatgaggta attcaaggta gaacttcgcc atgtccgttt
    55861 tgtacaaatt cgtatttaaa aaaagatgac atatatgaat gggaatttta taataaaaaa
    55921 ctgaaacgaa catttataat taaagaccgt atgttgaatt ggcaagggcg aagagttcgt
    55981 attgaattat cttatgatat gtatagtgct gaatataaat tagcaaaaaa agatcaggaa
    56041 agagaatcaa ttttgaaaac tattccagca ggaatggtta gattagatgc aagagattat
    56101 cttactatac tttggtgtaa tgatatattt ttgaatatga ttgaatatac aaaggaacag
    56161 tttgcagagg aacttaataa tcaatgtgga tatttatttt ctgaagatta taaacgtagt
    56221 aagaaactgg cacaagattt gaaggagtct ggggataatg ttgtatttga agcaaaaata
    56281 tatacacgtt caagggaaga acgtatatgg actgttacat tgtgttatat cagtggggaa
    56341 gacagttggg atggaatacc atctttttat agtataggta ttgacataac aaaggaaaga
    56401 aaacagattg agaaattaca gcatatatca gagaaagatg cattaacagg aatttataat
    56461 cgtgctgaga ctgaaaggca gattaagaaa tattttgaaa aaaatttaaa tgttatgggt
    56521 gcactgttta tgattgatac agataatttt aaacagatta atgatacaga agggcatatg
    56581 attggagata tagtacttac tgagatggca tcaggtatga aaaaaataat gcgtgacagt
    56641 gatgttgttg gaagaatagg tggagatgag tttacaattt ttatgaagaa tatttcctca
    56701 gttaaagatg cagaaaagaa agcagaagaa ctattgtata tgttccgtca tctatttcag
    56761 aaagaaaaaa gttttctaaa agtaacttgt agcataggaa tagctatata tccaaaggat
    56821 ggtactacat tcaaggaaat atatgcaaga gctgataaag ccctgtatca agcaaaaaat
    56881 atgggaaaaa ataattatgt aatctacaac caagattatt ttaaagaatt agaagaattt
    56941 gattgttctt ctcttggtac agttattgat tcagaaaaac aatacgctga atatcctgat
    57001 aatcttacac gttatatatt ccgtatgctt tatcagactg atgatataga ccaggcgatt
    57061 aatatgatac ttgaggttgt tgggaaacag tttgatgtaa gtcgtgttta tatttttgaa
    57121 aatacagaag atggtagata tacttctaat acttatgaat ggtgtaatga aggaatctct
    57181 tctcaaatgg cttatttgca aaacagcaat tatcaagact atgaggatta tgaaaagctt
    57241 tttgaggatg aattagtttt ttattgtcga gatatccata cattaagtcc taaactagaa
    57301 gaattgtttt ctagtcaagg tatccactct acacttcaat gtgcatataa agaagataga
    57361 gttttcagtg gatttgttgg atttgatgaa tgcacaggac tacgtctatg gtcacaagag
    57421 gaaataagta cattatcatt gatttctcaa attatatcaa tgtttttaca acgtaaaaaa
    57481 aataataaac ttaatcaaca gattcaacaa taccagaata ttttagataa tattgaccag
    57541 tgtatttgtg tgattaataa agaaaataat tttttattat atgtaaataa aaaatttaaa
    57601 aatacatatt ctaaattcca aattggacaa ccttattatc ttgattctat agatattcat
    57661 aagatatcca ttatatggaa taaaaaagaa gcatacctat gtactagcac gatttaatgg
    57721 taaagtaaag tttatattgg acttaaatta aatttcgttt taaataagat attattttag
    57781 ttgcttactg aaaagactag aatctatatt aatgaattat actaatttta taccactatt
    57841 aaaatattgt ttgctaaaaa taaaaaatag ccttaaaaca gttgattata aaaaattaac
    57901 tgctttaagg ctatttttat ctttgtgacg aaatagacac ttgccattta gaatgtgaca
    57961 atatagtagt tgtttagaaa aggcacaaag cattatactt taactaaagt aaaatgtatt
    58021 ttgcttttag aataaaaaca attaggaggg agctataatt gaaaaagata atatatgtat
    58081 tatttacaat tgaactatta atagtatctt tattaggtct aaatatagtt aaagataatg
    58141 aaattaataa tattttatat aacgatacaa caagtatatc tgtaatgttt aaagattata
    58201 aaaagttaaa taaaaattat agtaagtgga ttaaagatat tgcagatgaa aataatgtga
    58261 ctatatcaaa gtatgttttt aataataatg aaaaattatc tatatatacg actgatacaa
    58321 gtttgaataa caatataaag ttaaaatcag gaaaatttcc taatactaat tcaaatgaat
    58381 ttattagtaa tataaacact aataatagta atcaagtagg taaattctca actatgagta
    58441 aagacatatc aattttaatt agggattttg gtaaaataag tgaagttgga gaatcaggaa
    58501 tcttgtatat ttcaactcaa aatgaagata caataaataa aatattaaga gaactaaatg
    58561 tagacagaag tatttttgta gcaagattac atgatagata tgttaactca aatttatact
    58621 taaatccttc aataattaga gatttaatac taattatcct ttgtttcttg gctacaataa
    58681 tacattattc aattagtgtg agtagagagg cttctatttt aagattaaat ggatactcta
    58741 agctagatat aattgctaga gttataaaaa atatacttag aattatgatt ttatcatctg
    58801 tcacagcatt attaatatat ataatctata ttttaaaatt aaatataggt gttagagcat
    58861 gcttatactt tgctatgttt tcgattatat gtattttgat aaatattttg atatctatat
    58921 taatagttgg ttttaatagt agaagttcta aatatgtgct atctctaaat ggaaaaaaat
    58981 cttatggttt tgtaaatata atgcatttca cattgaaaat tgtatttgta tcatttctta
    59041 tattatccat aaataactgt attttaaatt ataatatgct acaaactcag ttaggaaatc
    59101 tatctgagtg ggataaagca aaaaatgttt ataatcttac tcttaaagat acaggcgaac
    59161 aatctttagg aaaggaagaa gtaattaaaa atgccaagat taaaggtttc tataaaaatt
    59221 tggtagaaga aaaagatgct ttcttaatag attcaactaa ttttgaaaaa cttgaagatg
    59281 gaagttacat gtatgaaata aattcaaagg gtaaaaaccc agaagtaagt caatatggta
    59341 aaaatataaa aataaataaa aactatctta aagttaaccc tatttactca aatggcaaag
    59401 aaatatttaa tctaatcgat tatggcgata agactataaa tttattagtt cctaaaaagt
    59461 tacaagtaca tgaaaatgag attaaaaaag agtttagaga attattttat tttgaaaaaa
    59521 tagaagtaga aaatatgtac aataaagagt taaatagaga gttaaataaa acaaagaagt
    59581 cagatttaaa tgttaatata atatatgtag ataataatca aagctatttt acttataact
    59641 cccttgttat ggatgacaat aggaatctca ttgatgaccc aatagcaata gttgacatag
    59701 ataatattga tgattctttt tatctttctt atatatctag atgtgtatat tttaattcta
    59761 agaaattaga tgcccttgca gatataagta acattattga agctcaaggt gtagaagcgc
    59821 atatccaaag tttacatgct gtttataatg agtatggttt agaaataaat aaactagaaa
    59881 aatatttgaa tagtgaaatt tttactataa taataatagc aatatcaaac ttaatgatta
    59941 cctacaatat agtagctagc tactatgaaa gaaataaata taagttatat attaaaaaaa
    60001 tatttggtta ttcaactaca gctagaagta tgttgttaat agtttcatta atattaacaa
    60061 acattattcc aataggcatt atatcaacaa gagtagattt aagtaataat attatcttat
    60121 ttggacttct aatattagtt atagaagtaa ttgtatctat tgcattagac aagattatct
    60181 caaatagttc atttaataaa attataaaag gagaacatta aaaagatgat ttcattaata
    60241 aatatatcta aaaaatttaa agataaatct ataatagaca aatttaacct ggaaatttct
    60301 aagggcgagt ttgtagcgat aacaggaaat agtggttcag gtaagacaac tttacttaat
    60361 ataatgggat tattagaaaa acctaattct ggtgatatta taataaataa tataaaaaat
    60421 ccaaatagta aacagataag aattttacaa agggattttt atggctatct atttcaaaat
    60481 tatgctttaa ttgagaatga aaatgttgaa aataacttga aaattgcttt aaagtatcaa
    60541 aaaaatatca accataatga aaaaataaat tacgcacttg agtctgtagg tcttagaaaa
    60601 tacaataaaa aaaagatata cgaattgagt ggaggagaac aacaaagggt agcattagca
    60661 agagtaatat taaaagaatg tgaagtaata tttgcagatg aacctacagg taatttagat
    60721 aaaaaaaata gagacatagt atttgatata cttaaaagtc taaataagaa tggtaaaact
    60781 atcgtatttg taactcatga tttagagtta tcggagcagg cagatagagt tataaaattg
    60841 taataatatc aactataata aatataagaa agagattaca gctaaatcaa gtttaataat
    60901 aatacgaata gaatttaaac attaagtaaa ttagtagttt gtaaatataa gtataatatg
    60961 atatcataaa ggctcaaaag aaattttata taataagaaa tctaaaaata acaaatggag
    61021 gacatataaa gatggaaaat ttaccaaatt gcccgaaatg taattcagaa tatacttatg
    61081 aagatggaac tcttttagtt tgtccagaat gctctcatga gtgggcatta ggattgggag
    61141 ctgaagaaga ttcaaatatt atcaaagatg taaatggaaa tgtattaaat gatggagatt
    61201 ctgttacagt aattagagac cttaaagtaa aaggaagttc atcatctata aagattggaa
    61261 caaaagtgaa aaatatccgt ttaatccatg actcatcaga tggacatgat atagaatgta
    61321 agatagatgg ttttggagct atgaagctaa agtcttctgt tgttaaaaaa gcgtaatatt
    61381 ataaataagc tgtagtgttt attgtttttc agtacagtat aaaaccataa gaatctatta
    61441 agtaataaaa gtttgtttat ttatctttat attttcttat ggttttatta aatataaatt
    61501 ttattgaaca ccaaaatatt ctctcctcta caaataagcc aatcttattt aaactaatat
    61561 actttgtact atttatactg aaattgtaaa tttaaatgct atgttattta ggtttgtata
    61621 gttaatttat atatttctga ttttataaat tgtatttatt attatatctt gattctaaaa
    61681 tcattaaaaa tttcaacttc tataaagatt tacaatttat caaatacatt atgagtatat
    61741 aaacaattta ttattacagt gctacattta aaataagtct tagtataaag gtattgttac
    61801 aagtataaag aaacttatgt atgctattgg aataaataat accttaaatt ataattaaga
    61861 ataagaactg ttaaataagg gacttgatta tcatttagtt atagataata taatattaaa
    61921 aataaagttt aagaggagaa aaaatatgat agatatacat aaaaatatat atgataataa
    61981 gttatttgag gagctaaaga ttgactgtaa gaagtgcttt ggactttgct gtgtagctct
    62041 ttatttttct gcttcagatg gatttcctat cgataaggaa tcaggaaaac cctgtattaa
    62101 tttacaacca gattttaaat gttctgtaca taatagtctt atgaagcgag gttttaaagg
    62161 gtgtactgca tatgattgtt ttggttcagg tcaaaaagta gcacaagtaa catataaggg
    62221 gatagactgg atgcaatcat cagaactaac aaatcaaatg tctgaagtgt ttttaattat
    62281 gagacaatta catgaaatgt tatggtatct taaagaagca tctgtattaa atatatcaga
    62341 tacaatcaaa tcaaaaattg atttaataat tgaagaaact gaaaagataa ctaatatggg
    62401 cccagaacag ataattaatt tggatatcat atcacataga acaaaggtta atttattatt
    62461 atcacaagcg agtgagtctg tgatgggaaa ggttaaatca tttatcaaga cttctactct
    62521 taaaaacatg aaaaaattaa gtaaaaatat agatttaata ggagcagacc ttagaaaaat
    62581 aaatctaata ggagcagatt taagaggtag atttctaata gcagcaaatt taagaaatac
    62641 agatttgagt ggggcaaatt taataggagc agacttaaga gattgtgata ttagaggtgc
    62701 taatcttgaa aatagtatat ttttaacaca attacaagta aatactgcaa aaggagattc
    62761 tagtacgaag ttaccagctt cactaatacg ccctaaatac tgggaaaaat aattaaatta
    62821 atacataatt ttacaaaata tgaacttttt tgactttaat tcatattata cattagagat
    62881 tctttacaag aatttcttaa tataatatag ggagagatat aaaaatgagt tatcaaaatt
    62941 ataaatgtga aagagattat gtgtatgatg attataaata caataagtgc aacaagtgct
    63001 ataatgatta tgaggaaatg acacatgttc atgaatattc agaaagtgtt aagttagcag
    63061 aagaatgtga ggatagacat aaccaccgtg cagcaggggt tactggtgaa gctataccaa
    63121 taaatggagg tacaaatcat gttcataaaa taaatgataa tgtagatttc cttgaccatt
    63181 tccataagat atgtgttaca acagggccag caattagaat acctggaact gataaacaca
    63241 ttcacttaat atgtggagaa actactgtta atgatggtca ttgtcataaa ttccttttta
    63301 ctactcaaat agaagcacct ttagtataag ctgatgtgaa ttattgtagt gaatttgcta
    63361 gattatagta taataatata taatagataa tatttaaact taatgaggac tatgatattt
    63421 aaccttcgta gtcctttcta agttttgtat aaaaaagtgt tatgcaatta aaagtgatat
    63481 agggaggaaa tttttatgaa atctggaata attcttgaaa tgaaacaaat aaattcaaca
    63541 gaatatgacg aaatgttgca agtctgggaa tcatcagtaa gagcaacgca tgatttttta
    63601 acagaaaaag atattgaatc tttgatacca ttagttgaaa ttggacttaa agaagtagaa
    63661 aatatagttt gtataaaaga taatgatata ataaagggct ttataggaat agataaagat
    63721 aaaattgaga tgctctttat agaagataaa tatagaggaa atggtattgg taaaaaatta
    63781 ataaaatatg ctatagataa atacaatgta aaatatgttg atgtaaatga gcagaataaa
    63841 aaagctgtag gattttatat tcacttagga tttaaagtgt ttgataggtc tgaaatagat
    63901 ggacaaggta acccatttcc aatactacat atgaaattaa tttaaagtta tacttctcac
    63961 ttattcgcaa aaatatttac ttaaattttt atacaaataa aagtcgtgac agttttagga
    64021 cacgactttt ttactacact attaaattga aatttatctg ttctatatta tgattattag
    64081 atgtagtttt tttattttaa ctatgtactt aaataccaag tatacccata cctatcaagt
    64141 acttcagcag cgccatcatt ttgatgtggt ggtgggtgtg gtaaaaattc ttgtacaagt
    64201 gtaccacctt cggcaagttt tacagatatt tcttctatcg cttctcgaga tggtagaaca
    64261 acacaaatcg ccatgccaga aattacaaca ggcttgtctg tatccgcacc cattatacaa
    64321 ccttcgtatt ttgtgaaagt aacacttgcg tgcataatag gattgtttgg attgctgtct
    64381 aatcgcgcaa atatagtttt tcctcctccg aaagcctcag cataaaaatt aaatgctttt
    64441 tcacaagtac cattaaatat taaatatgga gttagcatga ccaatatatc ctccttgata
    64501 tagaataatt tatagttgca cctgataatc taaattatga tttagtaaat tgtatcatag
    64561 ggaaaacttg aagtatataa ttttttatta ttatattata caaatttatt aatttaaaaa
    64621 aatgttatat gtacggaagg tttacaggat aaaatataat aaaaagatta aggtgacatt
    64681 ataaaaaatg tatattgaca ttgacgctac gtaattgtat atactttgtc ttaaaggaga
    64741 tggtaaatat gaatattata gaaattcgct cagataaaat atacaagaag ataatggatg
    64801 caccaataaa caaaaaagaa gatatataca gatatgaatt gatgaagcct tttgaattta
    64861 agtggaagtg tatgaatgtt ccaatagttg ctagacagaa aggtggatat gatgtaatta
    64921 tagcaagtga aatgttaggg gttttatcgc ctaaggatat tgatgaaaag caaaaaaaga
    64981 atataaatgt gttatctgct gataaaattt gggccacttg taaagaaacc atagaaaact
    65041 ctataaatgc ttttataaaa gaagggtatg atttaaacat taaggactat aaatattcaa
    65101 tattattggc gaatccaaat agtccttata caatattaag tgatggatac tggggtgatg
    65161 gtgggattcc tggatatata tttctatcat tggttcctaa tgaatatact atcaatagat
    65221 taccagtatt aatagcacat gaatgtaatc acaatattag atttcagttt atagagtgga
    65281 ataataatgt aacattagaa gaaatgatga taaatgaagg tcttgcagaa aattttgcaa
    65341 catggatgtt tggagaggaa atgttaggac cttgggtcag tagaacagat atcgaaacat
    65401 taaatactta tataaagcca ataataaaaa gtgctttaaa agaaactgga tttcaaaata
    65461 taacatctta tctttatggt gatgatatag ctaaaatgca aggatatttt ccagtagggt
    65521 tgccttattg tgcaggatat gcttgtggat attatatgat taagtattat ttagaaaaga
    65581 caaataaatc aataatcgaa gcgactttat tgccttatag tgagataatc gaagcagtaa
    65641 aagagttttg ggaataaatt ttataaattg cataatttga aataaattat gcaattattt
    65701 tatttttaag aaaaaataaa ttgacaataa tttataagaa atatacaata atatatataa
    65761 agaaatattt caatatttaa aagttttaag atttcatatc tagagtacct aacattacat
    65821 gttagtttct tgttacttat ctaattactt tatatttcgt agttatatat aaaattacta
    65881 taaaaaatga aatatattaa acaatcaact aaaaaatgaa atattaataa aaaaaaattc
    65941 aaattataaa tattaaatga atataattaa aacaaatata taaatttaag tataacactc
    66001 acattttaat gtattgtgaa atttttgttg aactttatgt accataactt ataaaattta
    66061 tgtcatgtta tatttgaata agtaataaaa ttgtagatgt aaattaatct acaaaattta
    66121 tatgaaatgg agttgatgct atgacagaat taatgattat aagtgggtta gtactattga
    66181 tttgtataac ttctagcaag gtattgtata aatttggagt accaatatta ttgattttta
    66241 tcttactagg gatgttattt gggtctgatg ggatggtagg aatctatttt gataactatg
    66301 aactgacaaa acaattatgt tctattggac taatatttat aatgtttttt ggagggtttg
    66361 gaacaaactg gaatatggct aaacctgttg ctataccttc tatattaatg tcttcactgg
    66421 gtgtaataat tacggctggc gttacaggta tgttttgcca catggtaata ggaactagtt
    66481 tacttgaagg tttattaatt ggggcaattg cagcttcaac agatgctgca tctgtgtttg
    66541 ctgtgttgcg ttctcaaaaa ctaaacttaa agggttcact tgcatcatta ttagaagttg
    66601 aaagtggaag taatgaccca atagcgtata tgttgacact aattcttttg acattgatga
    66661 ataattctgg ggtagaactt attgcaccaa tggtaataaa acaaatagct tttgggttag
    66721 ctataggatt tatactagca aaattatctg tttatatttt aagacattca aacttcgaga
    66781 tagaaggatt ttatactata tttataacag ctattgcaat actttcttat gcatttagcg
    66841 agtatcttgg aggaaatgga tatttaagtg tttacatttc aggtattatt ataggaaata
    66901 gtaggatacc tcttaaaaaa agtttggttc atttctttga tggaatttca tggattatgc
    66961 aaataatgtt attctttatg ttaggattgt tatctttccc ttctaagtta ccaaatgtat
    67021 ttggtatagc aatggccata tctatattca tgatagttat tgcaagacca ttagcaacat
    67081 ttatagttct agcaaaattt aaattctcaa ataaagaaaa attattcata tcgtgggtag
    67141 gtcttagagg tgcggcttca gtagtttttg caatttatgc tgttactcaa ggagtagcca
    67201 tagagaatga catattccat ataatatttt ttatagcgtt gctttcagta ggaatacaag
    67261 gttcattgat accaactgta gccagaaagt tggatttagt tgatgataat acacctgtat
    67321 taaaaacatt taatgattat gatggagaaa ttagctctaa acttatcgaa gtaaatgttg
    67381 atgagggtaa taaatgggct aataagagta taatagattc taatatacca gacgatatat
    67441 taatagttat gataaaaaga aaggaacagg ttttaatacc taaaggttca accattataa
    67501 aaacagggga tattttggta ttgagtggag atagaattga agaattaata caattataat
    67561 ttattttgta ttgactttaa cctaagggga aagtttaaac ttaaattaat caaatctaat
    67621 gcgcaggtga tgagatgttt tctataggaa tgttttctaa aataaataaa ataactacaa
    67681 agacattaag gtattatgaa gatatagggt tgttaaaacc agaatatgta gatgaattta
    67741 caaaatatag gtattataca acagagcaat tacctaaatt acatcagata ataactttaa
    67801 aacaaatggg tttatcatta gctgagataa aaaaagctgt agaaaatcct gaagaagttg
    67861 aaaatatttt aatgaataaa gaaaaagaaa tgttggatgt aattaaaagt gaagaatata
    67921 aaataattaa actacgtaat taccttatga atttgaaagg agaagtcaat atgaatgatg
    67981 taattattaa gtctttacca aaagttaaag ttgcttctat gagaaaagtg ttaaaggggt
    68041 atgatgaact ttttaatcta tttccaaatg taatggctga agaaatgatg aaaacagggt
    68101 gtacatgtgc agttcctgat tattgtttta atatatatca tgatggtgaa tatagagaga
    68161 ctgatatgga tgttgaaatg tgtcaagcta taaatgattt aaaggaagat acggatatat
    68221 taaaatttaa agaattagat gcagttgaaa atgcagtttg tatattacac aatggtccat
    68281 attcaacatt aggaaaaact tacgcagatg catttaaatg gatagaggac aataaatata
    68341 aagtggttgg aaatcctaga gaatcttata tagatggtat atggaataaa gagagtgaag
    68401 aagactggtt gacagaagtt cagataccag tgaaacgcat gttataatat gtctacaaag
    68461 tataaataag aataaaaaag gtggtgtctc aaagtaaaca ttttagttta tgagacactc
    68521 tttttcatat gaaatcaagt atatttttat aattacgatt atgaaaaaag gcttatctat
    68581 atttttagac agcctcttat ttttttatta taatagtttt ctaaagatgc tctatttata
    68641 aatttttatt aaagacttta taaataattg tatttaatat acaaaatata tataaatatt
    68701 gatgttttat atataaagtt gtatattttt ttaaggatat aaagtaaaaa aatatacaat
    68761 atcaagaaaa aaagatattg ggtatttaaa gtaatacttt atttaatttt aagtaaatcg
    68821 ggtatctaca tatatagata ctaatttcaa agaataggag ggtgtttata tggaaatatt
    68881 cgcaatagga cattcaaatt atccatatga taagcttatt aatatgataa aaaaatatga
    68941 cataaattgt gtagttgaca ttagagaaac tccatattct aaatataata ttcaatacaa
    69001 taaagaggca tttaacgaat ctcttagaaa ttccggattt ttatatatat acatgggaaa
    69061 agaatttgga gctaaaagaa ctaataaaga tgtatatacg caagaaggtt atgcagattt
    69121 tgagaaagta gcaaaagaag atattttttt aaacggaata gaaagattaa aaaagggttg
    69181 tcaaatgggt tataggatag ttttattagg agctatgcaa gaacctataa gatgtcatag
    69241 aagtatcttg gtgggaaaag tattaaataa agaaggtttt gatgtaaaat atataatgca
    69301 tgaaggaaat ttggcatatc aggaagatat agaggaatca ttattggata agtattttag
    69361 tgatagaaag caactgagta ttgacaattt attagggagt gcattaacga gagaagaaat
    69421 gatacaagaa ggttataagc tagctaataa agaaatagga tatagaactg agaaactagg
    69481 taaataaaaa tatacaagta gataaaatac agtacttatc ttaagtaaaa taatattatg
    69541 aatttataat agctaggcat gatgtgccaa ttaaaaatat tctagttatt taataatatc
    69601 attaaattta gggtattttt taatagcaca tcatgttgca gttttaaaaa ataaaatctc
    69661 taatattgcc ataatcaact gttctattaa gagtaggatg actagatata aatctaactg
    69721 taatttttga atttaacaaa caaggatttg aaattaaaaa tactctatct ccattgacaa
    69781 tagaagtaat agctactggc aaaatatcta caaatgtatc atatctgttt ttagcaattt
    69841 gataaggata atttcctaca aaaacaaata gatttaaatc atatatatta tcagtaaacc
    69901 aagcccattg accataaact ttgtcgatat tcattgtgcc taaaccaagt gtatcaaagt
    69961 atgttgctat agttaaataa atattttcca tgttttctaa atatgaaaca gtatatcttc
    70021 tgaaacgtat gggtgctgtg gaagaaatag gataaaccat atttacattt agattat
//
LOCUS       DAKBNN010000004        60851 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000004 DAKBNN010000000
VERSION     DAKBNN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.004"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            110..301
                     /locus_tag="OUB02_000251"
     CDS             110..301
                     /locus_tag="OUB02_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCT8884065.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            456..1865
                     /locus_tag="OUB02_000252"
     CDS             456..1865
                     /locus_tag="OUB02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCT8884066.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1996..2685
                     /locus_tag="OUB02_000253"
     CDS             1996..2685
                     /locus_tag="OUB02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCT8884067.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2688..3623
                     /locus_tag="OUB02_000254"
     CDS             2688..3623
                     /locus_tag="OUB02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCT8884068.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3620..4513
                     /locus_tag="OUB02_000255"
     CDS             3620..4513
                     /locus_tag="OUB02_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCT8884069.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4591..5337
                     /locus_tag="OUB02_000256"
     CDS             4591..5337
                     /locus_tag="OUB02_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCT8884070.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5338..6171
                     /gene="rsmI"
                     /locus_tag="OUB02_000257"
     CDS             5338..6171
                     /gene="rsmI"
                     /locus_tag="OUB02_000257"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCT8884071.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6194..6898
                     /locus_tag="OUB02_000258"
     CDS             6194..6898
                     /locus_tag="OUB02_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCT8884072.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7086..7514)
                     /locus_tag="OUB02_000259"
     CDS             complement(7086..7514)
                     /locus_tag="OUB02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HCT8884073.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7551..8981)
                     /locus_tag="OUB02_000260"
     CDS             complement(7551..8981)
                     /locus_tag="OUB02_000260"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCT8884074.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9113..9697
                     /locus_tag="OUB02_000261"
     CDS             9113..9697
                     /locus_tag="OUB02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HCT8884075.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9713..10234
                     /locus_tag="OUB02_000262"
     CDS             9713..10234
                     /locus_tag="OUB02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HCT8884076.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10300..10546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10630..12567
                     /gene="metG"
                     /locus_tag="OUB02_000263"
     CDS             10630..12567
                     /gene="metG"
                     /locus_tag="OUB02_000263"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCT8884077.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12597..13367
                     /locus_tag="OUB02_000264"
     CDS             12597..13367
                     /locus_tag="OUB02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCT8884078.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELSKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13705..14136
                     /gene="phnG"
                     /locus_tag="OUB02_000265"
     CDS             13705..14136
                     /gene="phnG"
                     /locus_tag="OUB02_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCT8884079.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14151..14726
                     /gene="phnH"
                     /locus_tag="OUB02_000266"
     CDS             14151..14726
                     /gene="phnH"
                     /locus_tag="OUB02_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCT8884080.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14729..15814
                     /locus_tag="OUB02_000267"
     CDS             14729..15814
                     /locus_tag="OUB02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HCT8884081.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15834..16703
                     /locus_tag="OUB02_000268"
     CDS             15834..16703
                     /locus_tag="OUB02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCT8884082.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16696..17562
                     /locus_tag="OUB02_000269"
     CDS             16696..17562
                     /locus_tag="OUB02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884083.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYIFHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17603..18316
                     /locus_tag="OUB02_000270"
     CDS             17603..18316
                     /locus_tag="OUB02_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8884084.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18317..19486
                     /locus_tag="OUB02_000271"
     CDS             18317..19486
                     /locus_tag="OUB02_000271"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCT8884085.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPSNGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19490..20326
                     /locus_tag="OUB02_000272"
     CDS             19490..20326
                     /locus_tag="OUB02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCT8884086.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20327..21322
                     /locus_tag="OUB02_000273"
     CDS             20327..21322
                     /locus_tag="OUB02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8884087.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21340..23028
                     /locus_tag="OUB02_000274"
     CDS             21340..23028
                     /locus_tag="OUB02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HCT8884088.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23040..24107
                     /locus_tag="OUB02_000275"
     CDS             23040..24107
                     /locus_tag="OUB02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8884089.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24591..24707
                     /locus_tag="OUB02_000276"
     CDS             24591..24707
                     /locus_tag="OUB02_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HCT8884090.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            24871..25938
                     /locus_tag="OUB02_000277"
     CDS             24871..25938
                     /locus_tag="OUB02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8884091.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEAAKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26130..26660
                     /gene="rnmV"
                     /locus_tag="OUB02_000278"
     CDS             26130..26660
                     /gene="rnmV"
                     /locus_tag="OUB02_000278"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCT8884092.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRYEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26831..27700
                     /gene="rsmA"
                     /locus_tag="OUB02_000279"
     CDS             26831..27700
                     /gene="rsmA"
                     /locus_tag="OUB02_000279"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCT8884093.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27766..29028
                     /locus_tag="OUB02_000280"
     CDS             27766..29028
                     /locus_tag="OUB02_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884094.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSSDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29179..30294
                     /locus_tag="OUB02_000281"
     CDS             29179..30294
                     /locus_tag="OUB02_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCT8884095.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30480..31376
                     /locus_tag="OUB02_000282"
     CDS             30480..31376
                     /locus_tag="OUB02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCT8884096.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31446..32243
                     /locus_tag="OUB02_000283"
     CDS             31446..32243
                     /locus_tag="OUB02_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884097.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32298..33650)
                     /locus_tag="OUB02_000284"
     CDS             complement(32298..33650)
                     /locus_tag="OUB02_000284"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCT8884098.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            33869..34747
                     /gene="purR"
                     /locus_tag="OUB02_000285"
     CDS             33869..34747
                     /gene="purR"
                     /locus_tag="OUB02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCT8884099.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            34903..35181
                     /gene="spoVG"
                     /locus_tag="OUB02_000286"
     CDS             34903..35181
                     /gene="spoVG"
                     /locus_tag="OUB02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCT8884100.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35394..36773
                     /gene="glmU"
                     /locus_tag="OUB02_000287"
     CDS             35394..36773
                     /gene="glmU"
                     /locus_tag="OUB02_000287"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCT8884101.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            36868..37818
                     /locus_tag="OUB02_000288"
     CDS             36868..37818
                     /locus_tag="OUB02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HCT8884102.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38662..38747
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            38909..39430
                     /locus_tag="OUB02_000289"
     CDS             38909..39430
                     /locus_tag="OUB02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCT8884103.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGEYKLIKVG
                     SKLVLQIGTNTEGVTLTEAQSAKLLSDIGEKKIYTSATTNSLGDPLTSNTKIDNKVLY
                     IVLIDNTVMDTTK"
     gene            39582..41258
                     /locus_tag="OUB02_000290"
     CDS             39582..41258
                     /locus_tag="OUB02_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCT8884104.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41270..42478
                     /locus_tag="OUB02_000291"
     CDS             41270..42478
                     /locus_tag="OUB02_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCT8884105.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42493..44205
                     /locus_tag="OUB02_000292"
     CDS             42493..44205
                     /locus_tag="OUB02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HCT8884106.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44198..45106
                     /gene="pilO"
                     /locus_tag="OUB02_000293"
     CDS             44198..45106
                     /gene="pilO"
                     /locus_tag="OUB02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HCT8884107.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45133..45699
                     /locus_tag="OUB02_000294"
     CDS             45133..45699
                     /locus_tag="OUB02_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCT8884108.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45736..46263
                     /locus_tag="OUB02_000295"
     CDS             45736..46263
                     /locus_tag="OUB02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCT8884109.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46284..47822
                     /locus_tag="OUB02_000296"
     CDS             46284..47822
                     /locus_tag="OUB02_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884110.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            47922..49004
                     /locus_tag="OUB02_000297"
     CDS             47922..49004
                     /locus_tag="OUB02_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCT8884111.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            48985..49767
                     /locus_tag="OUB02_000298"
     CDS             48985..49767
                     /locus_tag="OUB02_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCT8884112.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            49869..50519
                     /locus_tag="OUB02_000299"
     CDS             49869..50519
                     /locus_tag="OUB02_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCT8884113.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKVFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50533..51093
                     /locus_tag="OUB02_000300"
     CDS             50533..51093
                     /locus_tag="OUB02_000300"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCT8884114.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51110..54496
                     /gene="mfd"
                     /locus_tag="OUB02_000301"
     CDS             51110..54496
                     /gene="mfd"
                     /locus_tag="OUB02_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCT8884115.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54527..55522
                     /locus_tag="OUB02_000302"
     CDS             54527..55522
                     /locus_tag="OUB02_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT8884116.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55646..56185
                     /gene="spoVT"
                     /locus_tag="OUB02_000303"
     CDS             55646..56185
                     /gene="spoVT"
                     /locus_tag="OUB02_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HCT8884117.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56305..57918
                     /locus_tag="OUB02_000304"
     CDS             56305..57918
                     /locus_tag="OUB02_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCT8884118.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58025..59473
                     /gene="mazG"
                     /locus_tag="OUB02_000305"
     CDS             58025..59473
                     /gene="mazG"
                     /locus_tag="OUB02_000305"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCT8884119.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59562..59840
                     /locus_tag="OUB02_000306"
     CDS             59562..59840
                     /locus_tag="OUB02_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCT8884120.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            59912..60154
                     /locus_tag="OUB02_000307"
     CDS             59912..60154
                     /locus_tag="OUB02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCT8884121.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60216..60470
                     /locus_tag="OUB02_000308"
     CDS             60216..60470
                     /locus_tag="OUB02_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HCT8884122.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60471..>60851
                     /locus_tag="OUB02_000309"
     CDS             60471..>60851
                     /locus_tag="OUB02_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884123.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIY"
BASE COUNT        24538 a         6573 c        10991 g        18749 t
ORIGIN      
        1 attttttgtc aaaaaaaaca aaaaatacgt atttcttttt tcatttcggg aaaactaaac
       61 acaggaatta tggactttat aaaaatttaa ttcttgagga gtgatataag tgaaaaaagt
      121 tgctttaatt ttagctataa taggtgcgct taactggggt gcaattggta ttctaggtac
      181 agacttaata ggtagtatct ttggtggaac atatgaaatg gtaagtagaa taatatactt
      241 cattgtaggt ttagctggat tatacttaat accaagttta atgtctgatg atagagaata
      301 atttatgctt ttatgttaaa tacctcattc taaatgttta gaatgaggta tttaatttta
      361 taaaaatatt aatttgtttc ttttttaatg gtaaaataga taagaattga agttttaaaa
      421 agaagtatta tgtaaaaaag gagtactatt aatatatgtt tattattaat gaattaaata
      481 agattgtaaa tgaagaagta atatcttttc atatgcctgg tcataaaaag ggacaggttt
      541 ataaaaagtt aggatataaa gatatactaa aaaatttgta taaaatggat acaactgaga
      601 tattaggaac agataattta cactttccgg aaggaattat aaaaaattca caaaaaaaag
      661 catcaagagt atttaaaagt gattatactt gctatttagt aaatggtagt agctgtggaa
      721 tacaatctgc aataatgtca gtatgtagtc ctaaaagtaa aataataaca aatagagact
      781 gtcatcagtc tgttataaat ggatgtattt taggagatat agatatagag tatgtgcctt
      841 gtgagataag tgaagagaca aatatcttaa aagggatatc tataagtaat gtgattgata
      901 taatagataa aaacttggat gcaaaagcag tactgcttac ttatcctaca tattatggaa
      961 tgacatatga tttagaatac atatgtaatt atgcacatag caaaaagatg gtagtgatag
     1021 tagatgaagc acatggtgcg catttagggc ttagcgagag gcttcctaaa acagctcttg
     1081 agcaaggcgc agatatagtt gtacagagta cacataaaac tcttccagca tttacacaat
     1141 cgtcaatgat acatatacag ggagaccgag ttgatataga taaaatattg aacatgttaa
     1201 gaattacaga atcttcaagc ccttcatatt tattattatc ttctctggaa ttagcagtag
     1261 atatatatga aaataaagga aaagaattaa tggaagagct tttaaataat attataactt
     1321 tcaaaaataa agttgattca aatattaata tatacgacac aaatgataaa acaaaaatat
     1381 ttatatcatc taaaaacatg ggtttaacag gttatgaact agaaaatatt ttgagaaata
     1441 aatataacat acaagtggag ttatctaatt attatggagt tcttttaata tgtacaatag
     1501 gaaatactgc tgaggatttc tctagtttag aaacagcatt aaacaatgtt gtaaaggaag
     1561 actttaaatt tgaaaagtta gaaaataaaa aataccctgt tgttattcca aagaaaatat
     1621 taacacctag agaagcattt tataaaacta aaaaaagtgt taaaatatat gacagcatag
     1681 gtaaagtttg tggtgaatgt atagttccat acccacctgg aataagtgta atatctcctg
     1741 gtgaagtaat aagtaaagaa ataatagact atctaatgtt ttgtcattct aaaggcatga
     1801 ttgtgagtgg attgaaagat gtagatttaa attttataca agtaatagat ttagaagaca
     1861 tttaaaaatt atgatataaa agtagataga agtagatagt ttaaaattgt gaagagaaat
     1921 aaatagactg cttatataga gctatctaat aaaagtacta aaaaagtaaa aagtgtttgt
     1981 atggaggata tgaaaatgaa aggtaagtta ataattatag agagtggttc agatgcaagt
     2041 ggaaaggcta ctcaaacaga aatattatat gaaagattaa aaaaagaagg ctatatgatt
     2101 aaaaaggtag aatatccaaa ttatgaatct ccatcatctg ccttggtgaa aatgtaccta
     2161 aaaggagact ttggtaaaaa tgcatcagat gtagacccat atatagcatc aacttttttt
     2221 acagctgata ggtatgcatc atttaaaacc gattgggagg agttttacaa tgaaggtgga
     2281 ataattatcg cagatagata tacaacctct aatatggtac atcaggcctc taaaatggat
     2341 atttgtgata gagaagagta tttaaattgg ttagctgatt atgaatttaa tttatttaag
     2401 ataccacaac ctgattgtgt tatattttta gatgtgccta ttgaatttag taaaaagctt
     2461 atggagaata gaaaaaataa aattacaggg gaagaaaaaa aggatattca tgaaagtgac
     2521 atagagtatt tagagaaatc ttacaacaat gctttatata tagcagataa atatgattgg
     2581 aaaaaaataa attgtgtttt agataacaaa ttaagaagta ttgaaagtat acataatgaa
     2641 atttataaaa tagttcttga ttcaataaaa cctatggaga gataaatatg tattttgaaa
     2701 atattatagg gcaagatttt gcaaagaaat atttaacaaa ttctataaag aaaaataaac
     2761 ttaataatgc gtatatgttt gaaggaatgg atggaatagg aaaaaagaaa tttgcagatg
     2821 aattatcaaa actattactt gattatgaaa atctcgaaaa tagtccagac tatgtactca
     2881 taaaaccaga tggaaatagt ataaaaatag ctcaaatcag aaatttacaa tcagatatag
     2941 taataagacc tcataaagac tataaaatat atataatcaa taatgctgaa aaaatgactg
     3001 tggaagctca aaatgcactt ttaaagacgt tagaggaacc tccaaactat gctataataa
     3061 tcttagtcac aaacaacaag gaatctttat tagaaactat aaaatctaga tgtgatataa
     3121 taaagttctc acctatacca attgaagact taaaacgtta tttgataaat acaggtatag
     3181 aagaagaaag agctcaatta ttggctattt tttcgagagg aagtattgag aatgctctaa
     3241 atctatctca gtcttctgag ttttcaatga tgagagaaga tatacaacag tatattcaga
     3301 taatgttaga taaaaacata gtggagattt taaatatacc aaataatatg gaaaaatata
     3361 gaggtaagat tatagcttta ttagatatga tgataaacta ttttcgagat atcatcctat
     3421 taaaagaaaa tgtaaacaag aacatgttaa taaatgtaga taagctagtt ttcattcaaa
     3481 atatgagtgg aaaaattagt tattctcaat tgtccaagat tattgatata atagaagatg
     3541 caaagagtaa aataaaaagc aattgcaatt tcaatataag catacaagtt atgtctttaa
     3601 atatatacga ggtgattaaa tgataaagat agtaggtata aggtttaaaa gtgctgggaa
     3661 aatatattat tttgaccctg ttgattttaa tatagagcaa gatatggatg tagttgtaga
     3721 aactgcaaga ggcttggagt atgggaaagt agtcgttggt ccaaaagata tggatgaaag
     3781 cgaattagtt tctcctttga agccaattat aagaatagct acagaagaag ataaaaaagt
     3841 atatctagaa aataaggaaa aggcaaaaga aacttttgag ttgtgccaac aaaaaataaa
     3901 agaacatgag ttaacaatgt ttttaataga ttgtgagtat acatttgata ggaataagtt
     3961 gattttttac tttacagcag aaggaagaat agattttaga gaattggtaa aagacttagc
     4021 tgctatattt aaaactagaa tagaacttag acaaataggt gtcagagatg aagctaaatc
     4081 tataggaggt ctagggcctt gtggtagaaa actttgttgt tcatcttggt taggtgattt
     4141 tcaaccagtt tcaataaaaa tggcaaaaga ccaaagtcta tctctaaatc caacaaaaat
     4201 atctggaata tgtgggagat tattctgttg cttaaaatat gagcatgatg tatatagtga
     4261 agcaatagaa aaaatgcctg ttgtaggtgc attggtgcaa tcagatgatg gaaaaggaaa
     4321 agttgtagaa gtgaatcctt tattagaaca aattaaagtg gaatttcaag acaagacagt
     4381 aaagatatat acaagagaag atataaaaat acttagagag cctaaaaaat gtgatggctg
     4441 tggtggtaaa tgtaaggata atgatggatt agatgaagcc acactaagag agctaaagaa
     4501 attagaagat taaatttaag gtagggcttt tcctaccttt tttaaagcaa ttaatgctta
     4561 aagttattaa tatttgagga gaatttatat atgcaattaa aagattcaga aagaatagat
     4621 gatttacaat taaaaggcct taaaattata caagatacta atggtttttg ttttggtata
     4681 gatgcagttt tattagccaa ttttactaaa ataaaaaaag atgccaaagt agtagatttg
     4741 ggtactggaa ctggaattat acctattttg atagctggaa aaagtgaagc taaaaaaatt
     4801 attggcgtgg aaatacaaga agatgtatat gaaatggcaa ctcgttctat aaaattaaat
     4861 ggattagaag aaagagttga gattataaat gaagatataa aatacataga caaagtctta
     4921 gacataaatg gttaccatgt agtcacatca aatcctccat atatgcatat tgatggaata
     4981 aaaaatccaa atgataaaaa agcaatatca agacatgaag taaggtgcaa tttggaagat
     5041 gtaattagag ctgcatccag attggttatg cctagaggaa aattcttcat gatacataga
     5101 ccaacaagat tggttgatat aataacttta gggagaaaat ataaattaga accaaaggtt
     5161 attcaatttg tgcatccaag acctcaaaaa gcaccgaact tagttttggt tcaatttgta
     5221 aaagatggaa ggccagagct taaaatattg gacccattgt atgtttatgg agaagatggg
     5281 aattatacaa aagaattaaa agcaatatat aataataaag atataggaga gttgtaaatg
     5341 agcgggaaat tgtacatatg tccaacacca ataggaaacc tagaagatat aacatataga
     5401 actttaagaa tattaaaaga agtagacttg attgcagcag aagatacaag acatagtata
     5461 aaacttttaa atcattttga aatctcaaaa ccactaacta gttatcatga acataataag
     5521 gattcaaagg gagattattt gataaataaa ctaattgatg gtgaaaatat agctctgata
     5581 agtgatgcag gtatgcctgg tatatctgac ccaggagaag agataataaa acaagctata
     5641 caaaataaca tagagataga agtattgcca ggtgcaacag cctttgtaac ggctttagtt
     5701 ggctctggta tggacacaca tagatttgtt tttgagggct ttttagatag agataaaaaa
     5761 gttaggaaag cacaattgga agaattaaag gaagaaagta gaactataat tttctatgaa
     5821 tcaccacata gattgaagga tacactaaaa gatatgctta aaattcttgg taataggaat
     5881 atttctataa atagagaact tacaaaaaaa tatcaagaaa taataagaga aaatatagaa
     5941 actaccataa aaatatttga ggaaaaagaa gtaagaggag agtttgtatt aatagtagag
     6001 ggttttcatg gtgagaagtc aaagaaagaa aattatgatg ggcttacaga tagggaatat
     6061 gttttaaaac taattgaaag tggtatgaac aaaaaagatg caataaaaat agtttgtaaa
     6121 gatagaaagt taaagaaaga tgtggtttat aaacaagttt tagatttata aagcctgaat
     6181 ctgaggtgat tttatggaaa tagggaaaat tataatagag agtttaaaga gggaagatac
     6241 acataaaata tttatagaat tagataataa gcatgaatta gaaaaaataa ttcctaaaat
     6301 aagctgtatg aagtctgtag gtgaatgtaa atatcatgta gtaaattgtt ttgaacattc
     6361 tataaatgca ttaaaagaat tagaaatagt attaaatgat aaggattttt ttccaataca
     6421 tctaagaaaa catgtgtcaa actatttaaa cacatatatt gaagaaggaa taaataagtt
     6481 acatgtatta aagctgggaa catttttaca tgatataggt aagcctgaca gtatgacctt
     6541 agatgaaacc ggaagagttc atttcacaaa tcatgaaaaa ataggtgctc aaataattga
     6601 caatatggga ataaagttag agttatcaac agagacatat agacttataa gtaaatatgt
     6661 aagatatcat atgatacttt tgtctctata taagaaaaat gatttgagta ggaaagaatt
     6721 aattaatgta tttaacttag tagatgagga cactataggt gtaataatgc ttgggtatgc
     6781 agatatagtg tctacagtaa aattattggg taagagtgga gaagtcagtg tgttaaagac
     6841 atatatggaa tatattttaa caaattactt gtataagagt aattatacac atgtataaat
     6901 ttagttaata taattgagtt ttagatgatt aattaaagtt tgaaatatac ggatttcaaa
     6961 aacataaaag aagttgtctt tgttgtaaaa ttatgaaaga caactttttt tatatcaaaa
     7021 attgaaattt tattaaggat acttaatggt ctattaatta ataaaccatt agtatttaat
     7081 ttatattaca atattgattt tgcttgtatt ggcgttgata gtataacaga ggtcttagta
     7141 gaaccaattt tctttatagt atcaatcaca ttttctagct cgtacatatc ttttacaatt
     7201 acttttaaaa gtaagcaatc atcacctgtt atatggtgac attctacaat cctagggtcc
     7261 tttgcagctg actcaataaa ttctgtatat ccattgcttg gaagagaaat atgaataaat
     7321 gcctttataa ctctgcctaa tgaatctggg ttgacaatag ctttatatcc ttctataaca
     7381 ccagactctt ctaatctttt gactctttct gaaactgcag gagaagttaa accaactatt
     7441 tttcctaagt ccttcataga aattctccca tcatcctgta aaatttctat gattctgtaa
     7501 tctgtaacat ccataaataa acagtccccc ttaaataaaa ataattaatg ctataaaact
     7561 tttttagaat ctatcatttg tgtttttaaa taccaaagat ttctagataa atcaacctta
     7621 taaatgtgtg gttttgaaag tcttttgtat tgttcccaga tagtacttaa ttcatattgt
     7681 acatgatttt ttatttcaag aacagctttt ttattatatt tgcatctgcc ttttatatac
     7741 aatggtttaa gaagctcttt aactttgtaa tttgtaaata cctttgtttt ccatgtataa
     7801 gtaggatgga agatttctaa aggtttagac tcatctataa cttcatcatg taacataatc
     7861 aaatctgcct ctgccttatt gtcttcgtta tatattctta taactttctt gtaaccagga
     7921 ttatttattt tttctggatt ctcagaaatc tttatcttag gttcaagaac tcctttttca
     7981 caagatgcag ctaatttata aactcctcca agagaaggtg aatcgaaaga tgtaatgagc
     8041 tttgtaccta ctccccatga atttatagta gctccttctg ctttaagtga tgttatggta
     8101 tattcgtcta ggtcattaga tgcagtgatg ctgatatctg aaaagccagc gttatctaat
     8161 tgttttttag cttctttaga taaataagct aaatctccag agtcaagtct tatacccata
     8221 ggtttgtatc ctttttcaga aatatttttg aatactttta ttgcattagg aactccactg
     8281 tttaatacat cataagtatc tacaagtagt aggcatttat ctggataaac atcagcatat
     8341 gcttggaatg cttcaagttc attatcaaat ttttgtaccc aactgtgagc ttgtgtacca
     8401 attataggta tatcaaacat tttccctgct agtacgtttg cagttccagc acaaccacca
     8461 actacagcag ccctagcccc atatatccct gcatctggac cttgtgcacg acgtaatcca
     8521 aattcaaata cagggtctcc ttgagctgca aagcaaactc tagaggcttt agtagcaata
     8581 agagactgaa aattaactat tgttaacaat gctgtttcaa taagttgagc ttgatataaa
     8641 ggtgccttaa cagtaattaa tggctcatta gggaacatga tagttccttc ctcaacagca
     8701 tatatatctc ctgtgaattt aaattctttt aaaaatttta agaacttgtc agaaaataaa
     8761 tttaagtttt ttaagtactc taaatcttca tctgaaaaat gaagattatc tatgtattca
     8821 acaacctgat caataccaca aactatagta taccctccat cacaagcgtt ttttctaaaa
     8881 aacatatcaa atacaacaat gtcttcatga atattctttt caaaatagcc atttaacata
     8941 gtaagctgat ataaatcagt aagaagtgtt agatttctca ttatttctct ccttaattat
     9001 tattaaatat taaaaattgt aaaaactccc acacttatac taaaacataa tatagtacaa
     9061 ctttcaagta taaagtatta cctaactatc ataaaattat ttaaagagga ggatgattat
     9121 aataggaaaa atatggtttt atatggtatc gataggaata ataggaagct tgatttctaa
     9181 aaatatgggt gaattaaata aagttatatt aagtgaaaca tcaaaaggta tagagtttgc
     9241 aataagtcta gctggtatca tggcactctg gatggggata atgaatatcg caaaagaatc
     9301 aggtttaatt gaaaagatag gtcaaaaact aaatcctata atgagaagat tatttccttc
     9361 tattccaaaa ggtcataaag caatgtctta tatagttatg aatattgctt taaatatgtt
     9421 gggtgctgga aatggtgcaa cagcatttgg tttaaaagca atgagtgagc ttcaaacttt
     9481 gaataacaag aaagatacag ccactaatga tatgattatg tttatggtaa tcaatatatc
     9541 atctatacaa ataattccat ttaccataat caaactaagg atggatatgg gggctcaaaa
     9601 tccaagtgaa ataattttta cgactttatt tgcaaccata atatctacag cagtagcaat
     9661 aataacatgt aaattttttc aaagaagata tagataaagg ggatgtagat acatggagat
     9721 attttcaaac ttacttatac caataataat tctgtacata gtaatttatg gtaaatataa
     9781 aaaaatagat gtgtatgata gttttgtaaa aggagcaatt gatggattaa aagctgcatg
     9841 ggatatccta ccatatataa taggtatatt tttggcaata ggaatattca aaactggtaa
     9901 aggtcttgat atgttagaat ggctatttac accaatagca aatatgatga gtataccaaa
     9961 agaattaata ggtctaataa gtgtaaaacc tttatctgga agtggtgctt tagggatgta
    10021 cagtgaactt gcaaatagag ttggaatagg aagtttagta gaaaaaatgg gtgctacgat
    10081 tgtaggttct tctgagacta ttttttacac aatggcaata tactatggaa gtctgaagat
    10141 aaaaaatact agacatacat tatcatgtgc tatgattagc catgttgcag gtgtcatagc
    10201 agctgttttt atttgctatg taatatttgt ataatttgtt gacaatatgg gaaaaatttt
    10261 ataatatagt gattatatag aaacaaatag taatacaaat tacaagctat gaaaagaaga
    10321 gtaaataaag gttttttcta tcagagaact aggttaggtg aaagctagtg tcaaaagctt
    10381 tattgaagat ggtcttggag ctttttatct gagcaagtgt gctagggtaa aacggttgga
    10441 cacgttatag tctactaagg tatctataaa atgtttctac atttcatgtg aaacataact
    10501 attatagatg aattagggtg gtaccgtgaa taatctcgcc cctatgttaa ctcataggag
    10561 gcgatttttt attatttaaa tgaaagaatc atatgattac gaacgataaa aaattaggag
    10621 gtaaataaca tgagcaaacc gagtttttat gtaacaacac caatatacta tccaagtgga
    10681 ggattacaca taggacatac ttattcaaca gtagctgcag atacaatagc tagattcaag
    10741 cgtttctgtg gatatgatgt aaagttctta acaggaacag atgagcatgg tgaaaaaatt
    10801 caaaagaaag ccatagaaca aggaatgtca gagatagagt atcttgatgg aatgataaaa
    10861 gatataaagg ccttatggaa tactatggat atatcttatg atgattttat aagaactaca
    10921 gaaaaaagac atacagacat aatacaaaag atatttacta aactatatga gcaaggtgat
    10981 atatataaag gtgaatatga aggtagatac tgtactcctt gtgagagttt ctggacagaa
    11041 tctcaattac tagaaggaaa taaatgccct gactgtggaa gagaaacata tttagtaaaa
    11101 gaagaatctt atttctttag attatctaaa tatgaagaca gattaaaaga gttatttaaa
    11161 gatgatagtt tctgttttcc tgctgctaga aaaaatgaaa tggtggcaaa ctttttagac
    11221 aaaggattag aagatttaag cgtaactaga acgacttttg actggggtat aaaagttcct
    11281 tttgatgaaa aacatgtaat ttatgtgtgg gtagatgctt tatgtaacta tataacagca
    11341 ttaggttata tgactgataa tgatgaagaa tttaaaaaat attggcctgc caatgttcaa
    11401 atagtaggta aagaaatagt gagattccat acaataatat ggccagcgct tttaatggct
    11461 ttaggtctag aagttccaaa acaagtattt ggacatggat ggatactatt tgctgatgat
    11521 aaaatgagta aatcaaaggg taatgtggtt tatccagaac ctataataga aagatatgga
    11581 atagacacac ttaagtactt cttattaaga gagtttgcat ttgggcaaga tggaagttat
    11641 actcatagaa attttgtaac gagaataaat tatgatttag caaatgattt aggaaactta
    11701 ataagtagaa ctgtagctat ggttgaaaaa tacaataatg gtataatacc aacagcaaaa
    11761 gtttcaacta attttgatgc tgacttaaaa gaacaagctg tttcaactag agaaaacttt
    11821 gaagctgaaa tggataaaat gcaattccat gaagcattag agagtgtatg gaaattagtt
    11881 agaagaacta acaaatatat agatgaaact atgccttggg cactagctaa agatgaaacg
    11941 aagaaagatg aattagacac agttttatat aatttatgtg aatctataag aataatcgca
    12001 acactaataa atcctataat gaatgagact gctaataaaa tatatgaaca tataggaata
    12061 aaaggtcaag atgatataac tacatgggaa agtacaaaaa catttggtct tattggagaa
    12121 aatgtaaaag tatttaaagg tgaaccttta ttcccaagat tagatgtaga aaaagagata
    12181 gaagaattaa ctaaaatgtt ctctgggaaa cctccagtag aagaaaaacc tttagagcat
    12241 aaagaagaaa ttactattga tgatttagac aaaatagagc ttagagtagg gaaaataata
    12301 agctgtgaaa aacatccaaa agcaaataaa ttattagtat cacaagttaa gattggacca
    12361 gaaacaagac aaatagtatc aggaatagct gaatactata agccagagga tttagtagga
    12421 aaagaggtta cagtggtatg taacttaaaa ccagttaaat taagaggtgt agaatctcaa
    12481 ggtatgatat tagcagcagg tgatgatgga gacccatatg ttttaccatt tacacaaggg
    12541 gctaaagatg gatgcgaggt tcgttagtag gtaattaaat agtaaggaga ttaaagatgt
    12601 tatttgactc acatgcacat ttaaatgatg aaagttttga tgaagataga gatgaactta
    12661 ttggttcatt aaaagataaa ggtgtagatt tggttgtaaa tccaggagca gatatagaga
    12721 cgtcgataac agcaattgaa ctttcgaaaa aatacgactt tatatattca gcagttgggg
    12781 tacatccaca tgatgtatca aaactagatg atacagcaat agaaactcta agaaaacttg
    12841 caactgagaa tgaaaaagtt gtagctattg gagagattgg attagattat tattatgatt
    12901 attctccaag agaagaacaa aaagagtggt ttaagaagca aattgaatta gctaatgagc
    12961 taaaacttcc aataataata catgatagag atgcacatgg agatactttt gagataataa
    13021 agaatactaa aaatcctgaa ataggatgtg tacttcattg ttatagtggg aatgtagagt
    13081 tagctagaga atatgttaag atgggttgtt atatatcaat tccaggcacg gttactttta
    13141 agaataataa aaaaactaga gaagtagtaa gagaaatacc tctagaaaga ttattcatag
    13201 agactgattc accatatatg tcaccagagc cacatagagg aaagagaaac aatccttccc
    13261 aagtatcttt tgttgctgat aagatagccc aggaaaaagg gatatcttat gaagaagtat
    13321 gtagagttac aaaagaaaat gcaaagaaat tttttaatat aaaatagaca aattaaatct
    13381 tataatgata tatataataa gtcattataa gtataggatg agagtgcctc aaaatgagtt
    13441 ttttagttca tgaggcactc ttttttttat gaaatcaaat atattttcat catttcaaca
    13501 atgaaaaaga agtttatcat attttgagat agtttgtttt tatttattta attcatattt
    13561 taaaatatat tttcttaaca ttgttaaaaa agaatttaca agatattcat tagaattaac
    13621 tcaactctaa tattcactta acttaagttg tttattatat tattaaagag aagtaaacga
    13681 aaggtagatt aaggaggttg aattttgtat acagttgata gtactaagat attgataaat
    13741 atgaacttag agaaactaga aaagatatac tcaatgataa aagatacatc aagtatttct
    13801 ataattaaag aacctaactt ggctacagta atggtaagag ctaatgaaag tgtaaaaaac
    13861 acaacattta atttaggaga gatattagtt actgaatgta gtgtaaaggt agatgaaagc
    13921 ttgggttacg gaatagtttc agaaaacaat aataagaaag ccatttattt agcagttata
    13981 gatgcagttt tacatagcaa taacagtaaa tttgatgagt taaagaatta cataaataaa
    14041 agtgtatatg aagagaactt aaggtatgaa caagagatta ttgatgaatt tagcttgatt
    14101 aataaaacga aagttcaatt caatgctatg gattaaatag gaggagaatc atggtaaatt
    14161 atacacaaga aatatataga aagatgctag atagtgcttc atttcctggg aaattaaata
    14221 atatagataa tataaatata gaaaataata caaaactatt gaatggagca attggtatag
    14281 caatcacttt attagaccaa gaagttacct tctatataga aaattatact caaaaagata
    14341 taaaaaatat aagagcactt acaatgtcaa atcaaataga ttatagaaaa tcagattata
    14401 tatttttaga tattaattca gaagtggata tattacagat taaaataggt tcttttgaat
    14461 atcctgatga aagtgcaact atagtacatc aagtaaatga tttatcaacg tactctcaaa
    14521 ctaagtatat aaagcttgat ttgagtggtc ctggtataaa atctaaaaat agtatatata
    14581 taaatggaat tagagaagaa tttatagaaa gattatctag cataaataaa gattatccaa
    14641 taggaataga tttgatttta gtagataaaa aaggagaaat tgcatttata ccaaggtcat
    14701 caaaattaag ttgggaggta ttatagaaat ggcttatgta gcagttaaag gtggagaaga
    14761 agccatatta caggctaaaa atatacttga gttttatagg gtaaaaggag atagcttgcc
    14821 tatagatgta aagcagattg aggaacaaat gtcttatgct gtagacaaag taatgtctga
    14881 aggaagttta tacaacaagg aactatcagc attggctata aaacaagctc aaggtgatat
    14941 attagaagcc tctttctatg taagagcttt aaggtcaaca atgcctagaa ttacatattc
    15001 agaacccata aatacagaag aaatgagaat aaaaagaaga atatcagcta cttttaaaga
    15061 tataccagga ggtcagtatc ttggggcaac tagagattat gaaaatagaa tattgaatgt
    15121 agaattatta gaagaagatg aaaaaaagaa tagagatttt agagatagtt ttataaatga
    15181 attagaaaca gtagatgaaa tccctgaatt tacaaaaata tctaatatat ttagaaaaga
    15241 aaatttatta caagaagaag tttgtgaaaa caaattggaa gaagttattg atgatattac
    15301 aagaaatagt ttaagatttc cagtttctag gtcagcaaag ttacaggcat taactagagg
    15361 tgaaagtgga gtagtgtcag gttttgctta tgctattgta agaggttttg gtgatgaaca
    15421 tccatatata agtgaactta gaactggtaa tgtatttata aaagtaaaac atcccatcta
    15481 tgaagatgaa gaaataataa taggggatat gctggttact gaatgtgaaa tagtttcaaa
    15541 agcatcacaa aataagactg aaaattctaa aactaattcg gaagataaaa ataagatgtc
    15601 tttaggatat ggtctttgta tggggaataa tgagaataag gctatatcta tggccatact
    15661 agacaaatct ttggagccaa atagtgaaaa actagcacaa gatgaagaat ttgtattgct
    15721 tcatatagat ggaatagatt cattaggatt tatatctcac tttaagcttc ctcactatgt
    15781 agattttaaa gcggatgtag aaagaataaa atagtttgtg gaggtaagtt taggtgatat
    15841 ataactatgg ttttttagac gaaaacacaa aaaaagaaat aagaagaaag atattaaaag
    15901 caatatcaat acctggttat caagttccct ttggttcaag agagttgcct atagctaagg
    15961 gatggggtac tggaggttta caattaacac tatcattaat tggtaaaaat gatgtagtaa
    16021 aagtaattga ccaaggaagt gatgattcta caaatgcttg taatataagg agctttattg
    16081 caagtgtaag taatgtagaa accacaaaag atacattaga agctacattg attcaaacga
    16141 gacatagaat accagaagaa aaacttaaat ctaatcaaat actaatattt caagtaccaa
    16201 taccagaaac ccttagaata gtagaaccta gtgaagtaga aacaagaaga atgcattcag
    16261 aagaagatta tagtaggatg tgggtatatc tttatgaaga tatagttaga tttaatgata
    16321 tatctatagc agttgaatat ccatgtaaag taaatgatag atatttaatg aatccatcac
    16381 ctatacctag atttgatata aaaaaattaa atatgtcaga caatttattc ttgtttggtg
    16441 caggtagaga aaaaagaatt tatgctattc ctccatatac aaaggtagaa cctttagagt
    16501 ttgaggatta taagtttgaa gaggaaaagt ttgaaggaaa atactgtagt ttatgtaaca
    16561 gtacaaatac atttttagat gaagtatatg acagtgacac taatgaaaag tactatagtt
    16621 gctcagatac aagttattgt gaaaaagtta gattaaaaaa taacagtata gatgtgacaa
    16681 taggaggtac ttggaatgag taaaataaat gactctaaaa gattagaaaa tatagacaag
    16741 caagtattaa taggaagaaa tatcaatctt atttatggag atgggtgtga aaagtgtttt
    16801 gaaagcacag gatatgaaag caataatata tgtaagtatt gtggttctat agttgcatgt
    16861 aataatgtaa atttagaact ttatgaagga gaagtccttg gaattgtagg tgaatctggt
    16921 tcaggaaaaa gcactttatt aaaaatactg tttttccaag aacatgcaaa tagtggagaa
    16981 gtatatatat tccattacaa taaagacaca aatatactag agctttcaga ccaaaaaaag
    17041 agatacataa agaatcattt tatgggtatt gtgtatcaaa atccccacct tggtttaaat
    17101 ttagacttta gtagtggtgg taatatagca gaaaaattat tgatggcaaa tttatataat
    17161 gttgaaaaaa taagagatag aggcaaagaa cttttaataa aaactaatat acctgtagaa
    17221 agaatagacc ataaaccaaa atactttagt gggggaatgc aacaacgagt tcaaatagca
    17281 aaagctcttt caaataaccc tccaatattg ctattagatg aggttacaac aggtttagat
    17341 gtttctgtgc aagcggaagt attggattta ataagagaaa tccaaagaga acttaagatt
    17401 tctatgatag ttgtatccca tgactttgat gttataaaga tgcttgctga taggacaatt
    17461 gtaatgaaaa atggaagtgt gattgagtct ggtcttacag accaaataat ggaggaccca
    17521 caacacccat atactcagca acttataaat tcattgctat aaatatcgtt tgtaaataag
    17581 caaaatgggg gtttttaata aaatgaaaga aactatactt gaagtaaata attttagtaa
    17641 agactttgag ttacatgcat tgaacaagac tataacggca tgtagtaata taagttttga
    17701 agtttcaaaa ggtgagtttt taggaataat aggtaaatct ggagcaggta agtctaccat
    17761 attaaaaagt atctataaaa catatatacc aacaactgga gaaataattt ttaattcaga
    17821 aatatatggt aatgtagacc tggctaaaat tggagaccga gaaatgataa atcttagaaa
    17881 gaaagaaata ggatatgtat cacagttttt aaagacactt ccacgaataa ctgcaattga
    17941 actggtagta cattctttaa ttgagagtgg gtttgaaaaa gattgttcgt atgatatggc
    18001 aaaagatata cttaaccaat ttgaaataaa ggaaaattta tgggatgcgt atccaaataa
    18061 tttttctggt ggagaaaaat taagattaaa tttagctcaa gctatggtta aaaagcctag
    18121 acttttgtta cttgatgaac caactgcatc acttgataat caatctaaaa tatatgtaaa
    18181 agaaaaaatg ttggagctta aaagtcaagg aactacaatg ataggaattt ttcatgatat
    18241 agagtttatg gaaactgtta tagataagac ctttactatg gcaaaaggga ctataagtga
    18301 aagtggggta gcctagatga taaaactaat tcataatgca aatattgtat tagaagatat
    18361 gattttagaa aatggtcatt taatcataga gggagataca ataaaaaaag tatcaaatga
    18421 taagattgat tacgcatttg actttaatga gatagatgaa ataatagatg caaaaaactt
    18481 atatgtaata cctggtatta tagatattca cagtgatgcc attgaaaagg aaatagagcc
    18541 aagaccaagt acattattac catttaatat ggcattttat gaactagata aaaaactacc
    18601 ttcaaatgga ataactacag tttatcattc tatatcttta ggggatggag taggagttag
    18661 aagtattgat aactctttaa agatgattaa aaatattgat tcatataaaa atatagatag
    18721 taagagcata aatcataaag ttcatttaag atatgaagtg ttgtattatg aaggtttaga
    18781 aaaggtttta gaactattag atgaaaataa aatagactac ttatcaataa tggaccattc
    18841 tccaggacaa ggtcagtata caaatccaac attttataaa gagtatgcta ctaaagtgtg
    18901 gggagttaca gaaaattatg tagatacatg gttagatgat ttagtaaatc tgcatgataa
    18961 tttagattgg aataaaatag ctaatattat aggtatagcc aaaactaaaa atataaatgt
    19021 tgcatcacat gatgatgata cattagaaaa aatggatttc ataaaaaata taggcataaa
    19081 tgtatctgaa tttcctataa ctcttgaagt tgcaaaacat tcaaagaaat tgggtgttct
    19141 aacttgttta ggtgcaccga atattgtaag aggtaaatct cataataaca atttaaaagc
    19201 aatggatgct atattggaag attgttgtga cattgtgtgt tcggattatc ttccatcagc
    19261 tatgataaag tctatgtgta tagttgcaga aagaataaat aatttaaata aagcagtcag
    19321 tttatttacc tcaaatcctg ctaaagcagt tggtatatat gatgaaagag gtagtataaa
    19381 agaaaataaa aaagcagatt tagttttagt agatattgat agtgaatatc caaaggttgt
    19441 aaatactatt gtaaatggaa aaacagttta taaaagggag gtataaagat tgctaacaga
    19501 gttacattgt catacaagta tatcagataa taattttact actgaagaaa taataaaaaa
    19561 agcagcagaa aagaatataa atatcttagc aataacaaat catgacacat tatctggatt
    19621 ggatgaagct cttgtgcttg gagagcatta tggaataaca ataataccag gtatagaaat
    19681 ttcagcatat gattataaaa atagaaaaag agttcatatt cttggatata acatagattt
    19741 aaacagtcaa gaaataaaaa atttgtgcaa tccaatggta tgtgacagac ataaagcatc
    19801 tgttaagatg gtaaataaaa ttatagattt aggatacaaa atatctttgg aagatgtaaa
    19861 gaaatattca tcaaagactg gaatatttaa acaacatata atgaaagctc ttatagataa
    19921 agggtataca gataatatat attcaagtct atataaagaa ttgtttcata gaggaaacgg
    19981 caaggtatat gtatctttaa aatatgtgga ttacagagat gcaataaaag ctataaaaaa
    20041 ttctggtgga atttgtgtgc tagctcaccc aggtcaaatg gataatttta gcgctattga
    20101 agagatggta aaagtaggat tagatggaat agaagtatat catccatctc acaacaaaga
    20161 actagaaaaa gagtcgcttt cttatgctaa gaagtataat ctagtggtca caggaggctc
    20221 agattaccac ggattttatt ctcaacataa ttatgaactt ggaagcaaga gcttaaataa
    20281 agaagattta tttaagttta ggttagcttt aggaggggta ttataaatgt cttttttaca
    20341 agtaaaaaat gtaggtaaaa gttatggaca agttaaggtg ttaaaagaca tatctattga
    20401 tattgaaaaa ggagagttta tttgtttact aggtccaagt ggttgtggaa agagtacact
    20461 acttagaata attgcagggc ttgaggataa acatggtgga aaaataataa taaatgataa
    20521 ggatatgact aattcaccac ctgaaagtag aaattttggt atagtttttc aatcatatgc
    20581 attatttcca aatatgaatg tttacaaaaa catagccttt ggactagaaa ataaaaacat
    20641 aagtaaatct aatattgata aaaaagttaa agaagttcta gaagttgtag aattaagtgg
    20701 atatgagaaa aaatatccat cacaattatc aggtggacaa caacaaaggg ttgcattagc
    20761 tcgtgcgatt gctttagagc cagacttttt acttcttgat gaacctttat ctgcattaga
    20821 tgcaaaagta agacttaaac ttagggagca gattagaagt ttacatagaa aactaggcat
    20881 tacaactata atggtaacgc atgaccaaga agaagctctt tgtttagctg ataagatggt
    20941 tgttatgaat agaggagaaa taatacaagt tggaacgcca aaggaggtat ataaaaatcc
    21001 agaaacacct tttgtagctg attttatagg aacaataaat tttatagatg atggtattaa
    21061 taagatagct attagaccag aagatataaa agtagagtct aacagagatt ctaaagataa
    21121 ggatataaaa gtgggagaaa ttttagatat cgaatttaga ggatttaact atagaattac
    21181 agttgagtat cgttctaagc aaatgaaatt agatgtagtt tccaaagttg cagaacaaat
    21241 gaaactgtgc ataggttcta aaataaactt taagatacct aaagaaggaa ttgtacaata
    21301 taaatcagag ggatgtgctt aatagctagg ggtgaataaa tgataacatc aaaaaataaa
    21361 agaagaataa gtgatagcac aatacaaaaa tatttcttga ttttttcggt aattgtacta
    21421 atagtattta tattatttcc attagtatca ttattaaaaa atgcatttat ggatacaagt
    21481 ggaaatttta taggaatttc aaattttagt aaatatgttg aaaatccaag tcttatagca
    21541 tcatttaaaa attcaatttt tgtatctaca gtctcatctt taatatcact atgtttagca
    21601 tttatatatt catacgcaat aacaagaaca aacataaagt ttaaagattt ttttaaaact
    21661 atgggaatgt ttccactttt tgcaccaact atgttatatg gtatttcact tatatacctt
    21721 tttggaaata agggaatctt tacaaccatg ggtctggaag taccactata tggtccatta
    21781 ggaataatta tttctcaaat tctatttaca tttccacaag cttttttaat attatctgta
    21841 gcactttcga tggctgatta cagactttat gaagcagcag atagcttggg tgctagtgaa
    21901 tttaagaaat tttgcaaaat aacaattcct ggtataaagt actcattact tagttcattc
    21961 tttgtatcct ttatacttgc atttacagat tttggagcag ctaaggtagt tggaggaaac
    22021 tacaatgtac tggccactga catatataaa caagtagttg gacaatttaa cataccaatg
    22081 ggagctactg tttctatggt aatgttaatt cctgtactaa tagcatttac attagataag
    22141 atggcaagta agaaacaagg tatgaccata actgcaaagt ctatgccata taagattaag
    22201 gaaaataagt ttagagatag agtattttta atagtatgta gttttataac attgctaatt
    22261 tttattttaa tattcgtttc agttataggt tcagttataa agttatggcc atataattta
    22321 agctttacaa tggaacacta tacatttaaa ggtattttgg gaagtggaat aaaaacatac
    22381 ttaagcagtt tgaaaatatc aactttaaca gcaatatttg gaactatatt tgtatttttt
    22441 agtgcttata tgatagaaaa ggtggataag tttcctaaat taagacaaac agcttacctt
    22501 ttatcaatga taccaatggc cttacctggc ttagttttag gtatatcata tataactttt
    22561 tttaactcag caagtaatcc attaaatttt ttatatggaa gtacagcaat attagttata
    22621 gtaaatgtag ttcattttta ttcagtttgt tttataacct caaattcatc tttaaaaatg
    22681 ttggataaag agtatgaatt agttgcaaaa tccataaata ttccattcta taaagtattt
    22741 tttaatataa cagttccaat gtctataaca tcaatacttg aaatagtagt atattatttt
    22801 gtaaattcaa tggttactgt ttcagcatta atatttttat atacacctca aactcagact
    22861 gcatcaatat caatactaaa gttagatgag ataggatata taggaccatc agctgctatg
    22921 gcagtattag tgcttttaac aaatataata gtaagacttt tgtacgaatt tgtaacaaag
    22981 aaattaaaaa ataacacaca aaaatggcag caaaaagatg ttgcataggg gaggtaaaaa
    23041 tgaaatttaa gaagctagtt tctttaggag tattatcaac acttttatta agtgtaataa
    23101 caggatgtag tactgattca tcaggttcag attcaaagtc tgatagtaag acaaaagggg
    23161 atttaacagt ttacacagca attgaagaag atagtataga gccatattta gctacattta
    23221 aagagaaata tccagatatt aaattaaata ttgttagggc atcaacagga gatataacag
    23281 caagattatt agcagaaaag gataatcctc aagcagatgt tgtatgggga gttgcagcca
    23341 caagcttatt ggttgcagat gaccagggga tgttagaacc atatgctcca aaaggttctg
    23401 aagaaataga gtctgcattt aaagatgata aagaagttcc ttcttgggtt gggatagatg
    23461 cttggatgac aggtataaca gtaaacacta aagaattaag tgataagaac atcccagttc
    23521 ctagttctta cgaagattta ataaagccag aatataaagg tcttatatct atgcctaatc
    23581 catcttcttc tggtacaggg tacttaacag tatcagccct tatacaaatt atgggggaag
    23641 aaaaagcatg gcagtatatg gataagttac atgagaatat tggtgtatac actcaatctg
    23701 gttcagctcc agctacatca gcagcttcag gagaatatcc tattggtata tcttttggat
    23761 atagaggtat aaagttaaaa gaggaaggct atccagtaga agtagtattt ccaaaagaag
    23821 gttctggatg ggatatagaa gcaaatgctc ttgtgaaaaa agataatata aaagaagcat
    23881 caaaagtatt tttagattgg gctataagta aagatgcaat gaacgaatac tctaaaaact
    23941 atgcagttac tactatttct acaggaaatc caataccaga aggattccct aaaaagccat
    24001 tagagcaaat gatagataat gatttaaaat cagcagcaaa aaatagagaa gatatactaa
    24061 ataaatggat atcaaaatat gatggtaaga cagaaaaaga gagttaagta aaactataca
    24121 aatggagtaa aaagaaaaat ttttttaagt agattttatt ataataatat aagttgagaa
    24181 taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt ataccattca
    24241 gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat actacagttt
    24301 aaaatagtat attttaattt cattattaaa gtatagatgt tatatataaa gtaaataaga
    24361 tattaaataa ttataaacta aagtaaataa aatattgagc gattattaaa aagatatata
    24421 tatataattt aatttgagca gtttgttata tattataaaa aagagaaaaa taaaaggtaa
    24481 attgtttctg gataacttaa aattggaagt aagaatattt aactagagaa gaattattta
    24541 agctcaggtt agctctagga gtagctgatt ttataggagc aatagatttt atggatgatg
    24601 atataaacat gatagcaatt agaccagaag atataagggt agaatttaat agagatttca
    24661 aagaaattat agacctcaaa ctcagaccgt atcgatataa aaactaaagc tagatgatat
    24721 agtgttgtat ataggaccat cagctgttat ggcagtatta gtgcttttaa caaatataat
    24781 agtaaaactt ttgtatgaat tgataataaa aaaattaaaa accaagacat aaaaatggca
    24841 acaaaaagaa gttgaataag ggaggtaaaa atgaaattta agaagttagt ctctttggga
    24901 atattatcaa cacttttatt gagtgtaata actggatgta gtaatgtttc atcaggttca
    24961 aacttaaagt ctaataataa gacaaaagga gagttaacag tttatacagc aattgaggaa
    25021 gatagtatag atacatactt agctacattt aaagagaaat atccagatat taaattgaat
    25081 gtacttagat tatcagtagg aggtataaca gcaagattat tagcagaaaa agataatcct
    25141 aaagcagatg tgttatgggg tgttgcagct acaagtttga taattttaga tgacaaagaa
    25201 atgctagagc catacgctcc gaaaggcttt gataaggtaa atcctgcatt taaagatgat
    25261 aagaaagttc cttcttgggt tggaatagat gcttggatga ctggtataac agtaaatact
    25321 aaagaattaa aagctaaaaa tatatcagct cctagttctt atgaagattt aataaagcca
    25381 gaatataaag atcttatatc tatgcctagt ccagcttctt caggaacagg atatttaaca
    25441 gtatcagaac tcatacaaat tatgggtgaa gaaaaagcat ggcagtacat ggataaatta
    25501 cataaaaata ttggtgtata cactcagtct ggttcagcgc cagctatatt agcagcttca
    25561 ggagaatatc ccattggtat atcttttgga aatagaggta taaagttaaa aggtgaaggt
    25621 tatccagtag aagtagtatt tccaaaagaa ggttctggat gggagataga atcaaatgca
    25681 cttgtgaaaa aagataatat aaaagaagca gcaaaagtat ttttagattg ggctataagt
    25741 aaagatgcaa tgaatgagta ttctaagaat tttgcagtta ctacaattga cacgggaaat
    25801 ccggtgccag aaggatttcc aaagaatcca ttagaacaga tgatagataa tgatttaaaa
    25861 tcagcagcaa gaaatagaga ggctatacta aataaatgga tagaaaaata tgatggaaag
    25921 acagaaaaag agagctaagc cttacaataa aatttagtac aaaaagcgcc tagaatataa
    25981 gttttatatc taggctcttt tgatatattt atttaaaatg catatttcta tataaaaata
    26041 aatagtttag taccttgatt aaattaaata aaaaccttaa aataaagaga tgtaaagaaa
    26101 aactaaaaac gaataaaagg tgaatttata tgataaaaga aataatagta gtagagggta
    26161 gagatgacgt tacagcagtt aagagagcaa tagatgcaga acttataact acaggtggat
    26221 ttggatttcc aaaaggtgta atggagcgta taaaagctgc taatgaaaga cgtggtgtta
    26281 taatatttac agacccagat tttgcaggtg aaaaaataag aaaaaaaata gcatcagaag
    26341 ttccaggatg taaacatgca tttctaccga gagaggaagc taaaaaggat ggagatatag
    26401 gtattgagaa tgctactcct caaagtataa tagcagctct gaataaagtt agaactgaaa
    26461 atacagaaaa aagatatgaa tttaagcaag ttgacttaat tagaaatggt cttataggta
    26521 atgaagaagc atcacttaga agagatgcac ttggaaaaat acttggaatt ggatatggaa
    26581 atgcaaaaca atttttaaat agattgaata attatggagt tgaaagagaa gaatttatag
    26641 atgcattaaa acaaatctaa acgtaatgtt taatgtatga aaaatatgta tataaaataa
    26701 attgtggaat tacggttaat ttaagataaa ttatttataa tatatacctt attttaaatc
    26761 aatgtaaata catataaata agtataatta ataaaattaa atatcaaaat aagttaaaaa
    26821 ggagtaaata atggatagac tatcttcaca taatgcaaca aaggaagtag tacaaaaata
    26881 taattttaaa ttttcaaagt ctttaggtca aaatttcttg atagatagta atataattga
    26941 taaaatctta tctggagcta gaatcacaag aggagataat atcatagaag taggacctgg
    27001 tataggaact cttactcgtg agatgggtaa aatagcagaa aaagtagtgg ctatagaaat
    27061 cgatagaaat ctaattccta tattgaaaga taccttgtca gatttagata atacagaggt
    27121 tgtaaatcaa gatatactaa aagtagacat acaggaatta gtaaaagata aattaaatgg
    27181 tgggccagtt aagcttgtag caaatcttcc atattatata actactccaa tagttatgaa
    27241 atttttagag gaagacatac ctgtaacaga catagtagtc atggttcaaa aagaagttgc
    27301 agatagaatg aatgctattc cgggtactaa agattatggg gcattatcta tagctgttca
    27361 atactattgt gatacagaga ttgtggcaaa agctccaaga catatgttta taccacaacc
    27421 aaatgtagat tccacagtta taggtttaca tgtaagagat aaaagaaaat atgatgttca
    27481 taatgaagac atatttttta aaacagtaaa agcatccttt ggacaaagaa gaaaaactct
    27541 tcttaattcg ttgggaggtc ttggttttct taataaagat gaaataagag aagtattaaa
    27601 agaagctaat atagacgaaa aaagaagggg tgaaactctt agtatagaag agttttcagt
    27661 tctttcaaat ataataaata caaaagtatc ttctaaatag aaggtacttt ttttaatttt
    27721 gttaacatat attgtttaga gaaactgtgt taagggggga ttgcagtggg ttctaaaaga
    27781 aagtttaaaa gagactatat aatactagaa gctaaagaca taaactttag atacaaagaa
    27841 cgagtactgc caaaagcttt tgcaaaagtt gaagtaaatg atgaaaaagc aattattgca
    27901 ttatatgtag aaaatctaaa atatgttaaa gatgggtata aagcagtagc aataaaagat
    27961 gaccatggat taatagactt aggaaatata attttaaatg aacaaggaag aggtgaattt
    28021 gttttagatt tagaagatta tgatgatgag ataaaaggta ttgcattatt gtatgaaaga
    28081 aatatacctt taattggatt taaaggaaat aagcttgaaa attatgagga catattattt
    28141 caagaggtag aggaagaaga atatgaagaa tatgaagagt tggaagaaga agacgaagag
    28201 atagaggata atgaggaaag tgatagagac gaaggtagtg aagaagaata tattatagaa
    28261 gaatatgatg aagatgagta tgaagactat tgggaagatg atgaagatat tgaagaagga
    28321 gagttaaaag caagaacgga agtagaagat tctagtgatg aggaaataga agagttcaat
    28381 aattataagg aaatagaaaa ttataatagg gttaaagtac aggaagaata tatagacata
    28441 gaatatgatg aagaagaaga ggaagaagaa gtttttgaag taaatagaga agaagatagg
    28501 tttgtttcct caaaacctac taaatcaaag aaagttcaaa aagatcagaa taatattgaa
    28561 aaaataaaaa gagctaatgg agtaagtcaa ggagagagtg gaaataaaca aaccagtaca
    28621 ttacttatgc caagacaaat aaaaaaaggt ttgaaattat ttaaagaagt aaaacctttt
    28681 gtaacagatt atatagataa gacaagatgg tggaaaatag agataaatcc tacaacactg
    28741 tgtggatata caatgcctta tttaggatat ttaaacgtat tgaattacac tatgtatagt
    28801 gatgcagttt tacattcata taaatacaga cactatttat ttggagttca gtatgatgaa
    28861 tataataaga gaaaacatta tatttatgct atcccaggaa atagaaatga acagccagac
    28921 aatggaagca caggattttt aagatatcaa gcttgtgata atagaacaaa tagtttagga
    28981 tattggctat gttttataga ttctagacaa agaaatatag ttaggtaaaa ataataaaaa
    29041 tgaaaaaatt tagactcaat tcttatttta ctgaaaaaat taatgaaatg tagtacaaaa
    29101 ctttaaaaac tattgataat atttaaatat tacactacaa tatatcttga aatacctatt
    29161 atattatgga ggaaaattat gataaacgag caaagaattt taaatgagtt tcttgaatta
    29221 gtacagatag atagtttatc tttaaaagag gggaatgttg ctaaagtatt agttaaaaaa
    29281 ttagaagaaa taggttgttc agtagtaata gacaatgctg gtgaaaaagc aaatggagaa
    29341 actggtaata taatagctac attaaaagga aataaagaag gaaagaaaat attatttagt
    29401 tcacatatgg atacagtaac tccaggtata ggggtaaaac ctatagtgga tgaagcaaat
    29461 ggaataataa aaagtgatgg tactacaata ttaggttctg atgataaagc aggtatagca
    29521 gctatattag aaggcttaag atatataaat gaaaataata tagaacatac tgatatacaa
    29581 gttgtattct ctatatgtga ggaatgtgga ttagttggag ctaaaaattt agactatgga
    29641 aaaatagatt ctgaatatgc atttatatta gatagtggtg gttctccagg tgaaataata
    29701 gtaaaagctc ctgcacaaga tgtaataaat gttaagatat taggaaagac tgctcatgca
    29761 ggtcttgaac ctgaagctgg aataagtgct ataatggtag ctgccagagc aatagaaaat
    29821 atgaatttac ttagaataga cgaagaaaca actgctaaca taggaattat aaacggtgga
    29881 acagctacaa atatagtaac aggagaagtt aatataattg cagaagccag aagtctaaaa
    29941 gaaaacaaat tagatgttca aactaaacac atggtagaga cttttgaaaa agctgcaaaa
    30001 gattttggag ctcaaattga gatagatgtc aatagagcat acacaccaat agatgttagt
    30061 gaggattctg aaataataaa acttgcaaag aaggcttttt caaacttagg tatagaaggt
    30121 catactgaat ctacaggagg aggtagtgat acaaatatat taagtaagaa tggtatcgaa
    30181 gctataactt taggaattgg tatgaaaaat gctcatacac taagtgaaca tatagctata
    30241 aaagacttat atgattctgc taaaatggta gtggagataa taaaggaagc ataaatttaa
    30301 ataatagtgt ataatatata tatcgcctag cttaagctag gtgatttttc attttataga
    30361 agattgataa tctctattga aagcaaagag tatacatttg catataagag agtgtttaat
    30421 ttatgttcaa atgtaggttc ttaataatat ttagattgtt tcataaggga ggaataaata
    30481 tggaaacaag gtatgaagaa gcaaataaaa taacaataca atcaatactt tggaatatag
    30541 tattaacaat aattaaggtt atagctggtg ttattggtaa ttctagcgct atgattgcgg
    30601 atggattaca ttcagcatca gatattataa gttctatagg agtactaata ggcaattatg
    30661 tatcttctag acctggagat agagagcata attatggtca tgagaaagca gagacattag
    30721 tatcatttgt gttatcaata ttgcttattt tcgtttctat aacaataggt atagaggcaa
    30781 ttaaatcttt atttaactta gatgcattaa gtgttccatc aatattacca ttagtagtat
    30841 cagttatttc aatcctgata aaagagtatc aatatagaat aacaataaaa gttgctaaaa
    30901 agataaattc accagcccta aaagctgatg cttggcatca taggtcagat gctttatctt
    30961 cagtagctgc ttttataggt ataggtggtt ctattttagg gtttaaacca ttagacccaa
    31021 ttgcgtcagt tgtagtggct atatttgtag ctaaagtagg aattagtata cttataagtt
    31081 ctgttaatga attgatggat gtttcagttg atgaagaaga gataaaagaa ttaaaattta
    31141 ttgtagcaga tacagaaggt gttaaaaatc ttggtgatat aaagactaga aaacatggag
    31201 ctatggcata tgtagatttg actatatgtg tggatgaaaa tttgacagtt aagcaaggtc
    31261 atgatattgc taccaagctt gaaaaacata taataaaaca tatggaattt gtaaaaggca
    31321 taacagttca cgttgaacca tgtactaatt gccaaggaaa taagtgtaat aattaattaa
    31381 aacttaaaaa aaagtataaa aagttatata attgttttat gattataaac tctaggggtg
    31441 gttaaatgag caagaaaaga aaactaaaga aaaaagctgc aatattaata ggagtagttg
    31501 ttttctttat aatatacttt atatccttta gaataggaat aaacatggaa aaggataaaa
    31561 gtgaagtgcc aaagaaggta gaaaaagaag aagaagataa taggtcttta aggactcaaa
    31621 taaaagagaa gaagaaggtt tatatatcag ataaaaatat agaaaacata aaaattgaag
    31681 aagatatgtg ggatgaaata aagttatttt tctcagattt taagaaagta agaaatacta
    31741 cttcttctta cgaagcagtt tatgaaggtt attctgatga tggagttaaa ttttctactg
    31801 accttaactt ttttagagta tatactgtaa ataaagaaga atattataaa gtaccagtta
    31861 gttctaagga tgcttttaag aaacttttag atgagagtat atatacttcg tttgaccttt
    31921 taagtcatta taaagattgg aaagaggtta ctataactca tggaaaagaa attaaaaaag
    31981 ttcatagatg gaaatttgat gatttagcac ataagatgat ttcaaagagg gttgtaggaa
    32041 aagttcaacc tgagaaaagt aaggaaagaa gtgactataa ttttactata aatataaaag
    32101 gtgataattt tgatgtaaaa gttgatacaa tgggaaaaga ttatgtaaaa gttacttcta
    32161 agaaaaatgt atcttactat gaggttcata caagtttatt tgattacctt aaaaatgaga
    32221 tatttgaaat tggaaagaaa taagacttta agaaggctat caaattagat atttgatagc
    32281 ctaaattatt ttatatatta tttttcaacc aacaagtctg caaccttata aattggacca
    32341 gcaccagcag ttattactaa atcattatcc ttaacatttt cacgtaagta ttttactata
    32401 tcttcaaatt cttttatata gatggcatct acattgtttt gatataattt ctcaaccaag
    32461 tccttagaat gtatatctcc tgggtctttt tccctagcag catatatatc tgttatgata
    32521 actttatcag cagcatagaa tgcctctgaa aattcaccta aaagagattt tgttctagta
    32581 taagtatgtg gctggaagat acaccataaa gttgattttt ttaatttttt ggcagcagct
    32641 aaagttgctt ttaactcagt tggatgatgt gcataatcat ctactactaa agcattctta
    32701 tagtaccctt tagtttcaaa tcttcttccg acacctttat aaatttttat attttttctt
    32761 atagtctcta agtcaatacc agaaacaaga gctgccatta tggcagaaga agcattgtat
    32821 atgttatgaa gaccatatac agaaagttca aattctccta aatctcttcc ttcatattct
    32881 attctaaata ttccatgacc actattgtca aagtgaatat cttttattac agcatcattg
    32941 tcagaatctc tgccgtattt tataatagta gctttaacat catacaatat gtcatcaata
    33001 ttttcatcat cgccatttat tatgaaatag ccttctgggg gtaataattt tccaaatttg
    33061 ttaaaagaag ccttgatttc atctattcca gagaaataat caagatggtc ttcttcaaca
    33121 tttaaaacta tagatatctt tgggttaaaa ttaaggaaac tatctacata ttcacatgct
    33181 tcagttataa agtggttgga attacctatc ttaacattac cacctatcat acttaagtta
    33241 ccaccaacta gtatagttgg gtctaagtct gcatattcaa agattgtaga taacatagat
    33301 gttgtagaag tttttccatg tgttccagaa actgctatag aatttttata ttccctcatg
    33361 atttggccta aaaaagttgc tctgttcatt accaatttat ttttttcttt agcagccaca
    33421 agttcttcat tgtctggatg aactgctgct gtataaacaa ccatatttac atcatctgat
    33481 atattatctt ttttctgacc aatataaata gttgcccctt ggtccctaag tttgtctaat
    33541 aggtatgatt cgtttgaatc cgaacctgaa acttgataac ctttattgat acatatttca
    33601 gctaatgcac tcatgctgat accgccaatt cctataaagt gtatattcat aataaaacca
    33661 cctttctttt taattaagtc ctacaatatg aactatttct gtggtataat aatataatgt
    33721 tcaatatttt ataattaata tgcatataat attatatgta ttttattttt gaatataata
    33781 aaacataagt aatattatac cacaataaga attgttatgc attagttagt ttaacgttat
    33841 ataaaggtac ttgtaggagg aaataataat gaaatttaaa agaacagaga gaattggagc
    33901 aatagttaaa atactgtctg ataatccaaa taaaatatat actttaagtt attttacaaa
    33961 tcaatttaat gcagcaaaat caacaataag tgaagattta ttagttgtaa aaaatgtatt
    34021 tgagaagcta catttaggta aagttataac tatttcagga gcagcaggag gagtaaaata
    34081 tatacctaaa acttctatag cagaaaatgc agagttctta atggaattat gcgagagaat
    34141 atgtgacaag tctagaatac tatctggtgg ttttttatac ttgattgacc taatttacga
    34201 ccctacaata gctgctaaga taggaaagat attcgcatca aatattgagt atgtagatgc
    34261 agattatgtt gtgacaatgg aaacaaaagg tatacctatg gcacttatga ctgcaaaagc
    34321 tatgaattta cctctagtta taataagaaa agatataaag gtatcagaag gtcctacact
    34381 tagcatgaca tatgtgagtg gaaacagttc taaagttgaa agtatgagtt tgccaagaaa
    34441 agctcttaaa ccagatagta aagttataat tatagatgat ttcatgaggg gtggaggtac
    34501 tataaaagga atggtagacc ttatgaatga atttggtgca gaggttatag gtactggagt
    34561 attcatatct acaacgaatc cttcagaaaa aatggtaaaa gattatattt cattgattca
    34621 attggatgtt aatggagata aaatagtagt agaaccaaac ttaaagactt ttaaagatga
    34681 atatagaaat gaagatttag acgaagaaga agattcagaa tttgaatttg agatagatga
    34741 agattaataa aaaaaatatt tagaaaaata tgtaaaaata aaaagaggat atccctagtt
    34801 gttcatagaa ttatttaaaa aacagtttat attaatttat tccagcgata ataattttaa
    34861 tataaattaa tttctgtcaa aatcaaaggg ggatattttg gtatgaagat aactgacgta
    34921 agagtaagaa aattaacaga agaaggtaag atgaaatgta tagtttcaat aactttcgat
    34981 aatttgtttg tagtgcatga tataaaagtt atagaaggcc ataatgggct atttatagca
    35041 atgcctagca gaaaagtagg cgaaggaaat ttcagagata tagctcatcc aataaatgca
    35101 gaaatgagac aagttttaga agatgcagtt ttacaagcgt atcatgaagc attagttcaa
    35161 tgggaagtag ctgctgaata ataataaata tataaaatgg agagagcccg aacatttagg
    35221 ctctttttaa atgtagaatt tctattaaaa tatgtacttt ttagtaaaaa agtgctatat
    35281 tattagtgat aatgtatatg ttgtatacaa attgttagat aaatgtatta taatgtatat
    35341 tgattaagag aataatacga ataaaattaa attagattga atggagtgta tcaatgaact
    35401 ttaaagctat aatccttgct gctggcaaag gaacaagaat gaagtctaaa tacccaaaag
    35461 ttatacataa agtatgtgga aaagaaatgg ttaaccatat tatagatgtt tcaaaaaaat
    35521 caggggtaaa agatactgtc gtgattttag gacatgaagc tgatgtagtt aaagaaaagc
    35581 ttgcagaaga aattataata gccatgcaaa cagaacaact tgggacagga catgctgtaa
    35641 agatggctaa agaatatatt aatgatgaag atacaatagt tgtactttgt ggagatactc
    35701 cacttataaa agaagagaca ctaaagagac tatttgaata tcatatagaa aataaatatc
    35761 atgctacagt tcttacaaca agagttggaa atccaacagg ttatggtaga ataataagag
    35821 ataagaaggg tgaccttctt aaaatagtgg aacaaaaaga tgcaaattca gaagaaaaga
    35881 tgataagtga gataaactct ggaatttatt gttttaatgg aaagagtctt agagaagcac
    35941 tagatttgct aaataataac aattcacaag gtgaatatta tttaactgat actgctaaaa
    36001 taatgagaga taaaggatta aaagttggtg catttgcagg ttcaactatt gaagaactta
    36061 tgggcgttaa ctcaagagta gaattatcaa aggcagaaga aattatgaga cgaagaataa
    36121 atgaatctca tatggtaaat ggagtaacta taatagacac taactctacc tatatagaat
    36181 ctgatgttat gataggcaat gatactataa tatatccagg tgttatgtta caaggaaaaa
    36241 caaggatagg ttcagattgt ataattggaa tgaattcatc tataacaaat tcagaaatag
    36301 gtgatggtac agaaataaaa aattcaacta taattgatag taaggttgga gaaaattcta
    36361 ctgttgggcc atatgcatac ttaagaccaa agagtgactt aggaaataat gtaaaaatag
    36421 gcgattttgt agaagtaaaa aatgctatta tagaagatgg ctcaaaagca tctcaccttt
    36481 cttatatagg agatgcacat gtcggtaaaa atgtaaatat tggatgtgga gtagtatttg
    36541 taaactatga tggtaaaaat aaatttaaat caatagtaaa agacaatgcg tttataggtt
    36601 caaattctaa tttagttgca ccagtagttg tagaagaaaa aggatatata gcaacaggtt
    36661 caacaataac acatgatgtc ccagatggtg ctttggcaat agcaagagaa agacaagtta
    36721 tcaaagaagg atgggtagaa aaaaagaatc aaaaagacga ccaaagtaag taattatgta
    36781 gtttattatt ttggctaaaa aatttagctc gaaattatta taaattatat tcattagtat
    36841 tttaaaattt tcaggagggt tacataaatg aatactagcg gaagcgagat taaaattatt
    36901 gcaggtaatt catcgaaaga gttagcacaa aaaatagctg actatatagg tgtatcagta
    36961 ctagattgtg aagtaggtac atttagtgat ggtgaaatat gtgtaaacat gaatgagaca
    37021 gtaagaggtt gtgatgtgtt tgtagtgcaa tctactaaca gcccagtaaa tgataactta
    37081 atggagttat taatcttaat tgatgcgtta aagagagcat cagcaggtag aataacagca
    37141 gttattcctt actatggata tgcaagacaa gacagaaagg ctaaggcaag agatccaatc
    37201 actgctaaat tagttgcaaa tttaataact gctgctggag cagatagagt attaacaatg
    37261 gatttacatg cagctcaaat acaaggatat tttgatatac cattagacca cttactaggt
    37321 ggaacaatat tagcaaatta ttttaatgaa aagaaaatag aagatttagt agtagtatct
    37381 cctgacttag gaagtgttac tagatcaaga aaatttgcta atactttaaa tggagaagtt
    37441 ccaatagcta taatagataa aagaagaccg aaagctaatg tatgcgaagt tatgaactta
    37501 ataggtgatg ttaaaggtaa aaatgttata ctattagacg atatgataga tacagcagga
    37561 acaatagtta atgctgcaaa tgcacttaaa gagtttggag caaaagatgt ttatgcttgt
    37621 tgtactcatg gtgtgttatc aggtccagct attgaaagaa ttgctaattc agaaataagt
    37681 gagttaatag ttcttgatac tatacaactt cctgaagaga aaagaataga taagataaag
    37741 ataaaaacag tagctccttt atttggagat gctataagaa tgatattctc taatgaatca
    37801 gtaagtaaat tattctaatt tattgtattt atgagagttg tttaaaagga tataaacctt
    37861 ttgagcaact cttttttgat tttataatta tggaatgtta tttttttctt gttagaaagg
    37921 tatatattat tgtaaaaaga gattatagtt taaaattatg tatatttaag taaaataaac
    37981 ttaataaagg gcatattatt caaaatatta tgaatataaa aaatattaca aaaaagtatt
    38041 gaaaaaattc agattatgta gtattatgta aatataaata aagaaagaaa gaggtgtttc
    38101 caatgggaag gttagaagat gaacccctaa taattaaatg attcagtcac aatctaaagt
    38161 gaatataaaa atctattgaa agtttattta taagaattga aaatagtatc tattctatct
    38221 ttagtgtttg tgagagggtt ctggattata taagattact gtaaagaagt aattttgcac
    38281 aataagcata tctgtaaatg aagagtttcg tttatataat gattgtggct gaatcataac
    38341 tataatagat agaatgttta taatagatga ttttataaaa tactatattg taatagcttt
    38401 tagctttcaa ataaatatta gcaaaggatg gaaatgacaa tgataaaact aaatgataat
    38461 ttataaatat ctccttacga tatataataa ttgtagggag atgtttcttt tttttgtaga
    38521 gtataatttt tctagaaatt ctgtttcaat atgtaaaaag ttggtatata taaatagtat
    38581 aaacagtgtt gctaaaatat ggtagatatt atataatata aatggatatg tatatacatt
    38641 atgagttata taatttgaat ttaataaaat agaaacgttg atttatgttc tgtaatgtgg
    38701 gcaccttgga gcatattgag ttagtggtgc aaccggctat gaaattgtat ttatttataa
    38761 atactattat tttcatagct ttttttattt aaaaaatggt tatatgttag aaataaattt
    38821 ggttagtacg tttaaataat tttacttaga tttttaacca gatatattat aagtaagtat
    38881 taattaaaaa atttaagggg gaataaaaat gaagttaaaa aagaataaaa aaggtttcac
    38941 tttagtggaa ttattggtag taattgcaat tataggtata ttagcagtag tggcagttcc
    39001 agctttattt agtaatataa acaaggctaa ggtagcaagt gttgagtctg attatagttc
    39061 agttaagagt gctgcattat cttattattc agatactaat aagataccag ttacaccaga
    39121 tggtcaaact ggtttaagtg ttttagaaac ttatatggag tctcttcctg ataaagctga
    39181 tataggtgga gaatataaat tgattaaagt tggtagtaaa ttggtattac agataggtac
    39241 aaatactgag ggagttacct taacagaagc acaatcagca aaattattga gtgatatagg
    39301 tgaaaaaaaa atatatacaa gcgctacaac aaatagtttg ggagatccat taacaagtaa
    39361 tacaaaaata gataataaag ttctatatat agtacttata gataatactg tgatggacac
    39421 aacaaaatag ttaaaacaat atttttataa agaatattgt aaatttaatg aaaaatataa
    39481 tataaaagac aggaagaaaa tcttcctgtt tttttatact attttcttta atgttcaatt
    39541 atgaatgatg atataattga gatagtaaag gagtgaaacc tgtggctaaa aaagttagaa
    39601 taggagacaa actggtcgaa aaaggatata taacagaaga acagcttaaa tgggcactat
    39661 cagaacaaaa aaacagtgga aaaagactag gagaatttct agtacaagaa gggcttatag
    39721 atagtaactt actaataagt gtattaaaag aactcttgga tatagaaagt atatttttag
    39781 aagggacaga aatagacact ttagctacaa aaatggttcc agaaaatata tgcaaaagat
    39841 atactgtatt tccatttaaa attgatggaa ataaaatatg tctagccatg tcagacccac
    39901 aagatagaga agcagttcaa gatgtaaggc gtatgtctgg taaagacgta gagatattta
    39961 tatcaagtac agaagatata aacaaagcaa taggtcatgc ttatgcacat tcagaaataa
    40021 ataaggcaat gactgagtac aacaaaaata gaactggtgg agtaagagaa acagtaatac
    40081 ttgaagaaga tgtaaatgct gctccaatag tacgattagt aaataacata ctagaaaatg
    40141 ctgttagaat ggaagcaagt gatatacata tagaacaaag tgaaaattac atgcgtgtta
    40201 ggtttaggat agatggaatg ttaagagaat atatgaggat gaactcagca ccatataagg
    40261 cagtaatcag ccgtatcaag ataatgtcag atataaatat atctgaaaag agaataccac
    40321 aggatggtag aatctattta aaagtagata ataaacccat agattttagg gtttctacaa
    40381 tgccaactaa cagggatgaa aagatagcta tgagagttct tgataagagt aattttatgg
    40441 ttagtaaaga agtactagga atagacgaac atggctctaa gatatatgat gaattgataa
    40501 acactcctta tggtcttata ttggttgttg gccctactgg aagtgggaaa accactacat
    40561 tgtattcaat gttaaatcaa ctaaatacag aaaatagaaa tctattaaca atagaagacc
    40621 ctatagagta tgaattacct ggtgtaaatc aatctcaaat aaatgaaaag gcagggctta
    40681 ctttcgccag cggacttaga gcttttatgc gtcaagaccc agatataata atggttggag
    40741 agataagaga tactgagaca gcagaaatag caattagagc ttctcttaca ggacatttag
    40801 ttttaagtac attacatgca aatacagcag ttggagcaat ttcacgtctt ttagatatgg
    40861 atgtggaaag ttttttaata acttcatctg ttttaggtgt aatatctcaa agacttacac
    40921 gaaaaatatg tgagcattgt aaggtttcat atgaagcaga tattggagaa aaaaaggctc
    40981 ttggtattga tgtaaatgaa tcagtaacta tttatagagg aaaaggttgt gaaaggtgta
    41041 ataatactgg atataaagga cgtctaggta tatttgaaat gcttgaaatt actccagaaa
    41101 taaaagagtt aatagattca tctgcaaatc aaagagaaat tttaaagatg gctagaaaac
    41161 aaggtatggt tagcttaaaa gaggatatag ttaaaaaagt tttaaatgga aagacaacag
    41221 tagaagaaat gattagaata attttgatga ctgattaaag gatgatatta tgaaaagttt
    41281 taaatacaca gtaataaaag aggacggaaa aaagcaaact gatgtgattg aagccaacga
    41341 ttttaatgaa gctagaaatc ttcttagaaa gagaaatctt agagttattg aaatcaaaga
    41401 aagcaaaaat aaaaatataa gattatctag tagaaagaga aaaaaagact tgggagctga
    41461 ccagataagc cacttttgca gacagtttgc tattatagta tcttctggta taaatagtat
    41521 ttctggatta gaaacattag ctagaaggtc aaccaacatt acattaagag aagaaattaa
    41581 tcgtatagtg gcagaaataa aagtaggttc tactattgca gattcaatgc tttcatcaaa
    41641 atcaaagttt ccaaagttac tcggtgctat ggtagctaca ggtgaagcta caggtaaatt
    41701 agatgaagta ttaaaaagta tggcttcatt ttatgcaagt gaacacaggg ttaaacaaaa
    41761 gcttagaaat gctgcaacat atccattaat agttttgatt tcttcattta taatgatttt
    41821 tatatttaca acatttatgg tacctaagat gattaattca attacaacag taggagcaac
    41881 tcttccactt ataacaagaa ttgttatggg gtttggtatg tttatgaaga agttttggat
    41941 aatagtactc ttagttatta tattatttat ctatcagttt aaaaaatatc taaaaacgtc
    42001 agttggaagg gctcataaag atagagttat aaataagata cctgttttag gaaaaggaat
    42061 aaattgtatg gtagctacac gattttcaag agcattgtat ctatttgtat caacaggata
    42121 cccattagtt caagggcttg attatattat agatagtgta aataacacca tggcagagaa
    42181 gattttagca tcagctaaag atggaataac aagaggagaa ggcttagcag aaaatttaga
    42241 gagatatacg tattttgact ctgtgttggt acagatgata gctatagggg agcagacagg
    42301 ggaattagag aatataagta gacagatggc agaattttat gagtatgaat ccgagattta
    42361 tttaaatagg atggcctcaa tgatagagcc tgttttaata attgcagttg gtattatagt
    42421 tgcgatactg gttgtatcaa tatttatgcc aatgcttagt atatatgatg ccatgtagga
    42481 gggaaaggac aaatgaacaa ggtttatgta tcatattatg gcgattctat ttccatagtt
    42541 gagggcagat ataaacacga taaggataag ttttatgtaa aaaattttaa tgtgttttca
    42601 atagaggata ttgcacaaga tttttctaag gaaaagtatg ctctattgag atacgcatta
    42661 gaaaatgtta agttaaaatc aagacatgta gttttttgtt taaatacaag agatgtaatt
    42721 ttaaaacccc aaaaacttcc taatatggac aaaaaggatt tagatggatt tatgaagcta
    42781 gagatggacg aaatgatggc tttggagtca gatgaatatg tattttcgta tgaagtatct
    42841 agtgaaacac ctgataattc agatggcgat gaatcaagct taaatcttat cattggagct
    42901 atacaaaagg aagaggtaaa tgcaattgta gagataatgc atgaatttga tttagtatta
    42961 gatagaatag atacattatc aacagcttac ttaagaatgc ttaaaaattt agactacaat
    43021 gatataatgg taaccaatat aagtgacaat agcacaatta taaatatata taagaaagat
    43081 actttattta ttagtgataa tatacctata aagataactg ctaaagatat ggacatgcaa
    43141 ttattaaatg tagtagaaga atcaaaaggt cttatgaatt actattcgtc aagaaacttt
    43201 ggtaaggtta cggatactat attaatatta ggaaaaagat attccaataa attaatttat
    43261 gaaactttta gctctgcatt ttcaagctat gtttctcaag gccttactga gataatggat
    43321 atatctgata tgataactgg agatatagat gaagatgata taaattcaat tgtagagctt
    43381 ttaggaagta tgatagaacc taaagggaaa agagattttg aaaatataaa ctttttacca
    43441 atggatatac ttagaaaaca gataaaagct aaacaaatga aaaacatact gaaaattgct
    43501 ccaatagtta ttttgattct tgctatacct tatttatgct taattttttc aaataattta
    43561 gcaaataagg aacttgaaaa tgttaatggt gaaataagaa atattgaatc tgcatattat
    43621 cagattggaa atatagaaaa aatgatacag agtaaaagcg aagaaataaa ggtatatgat
    43681 atgttaattg ggaaaaaggt aaagtgggaa aatttattgg atgatataga taaaaatact
    43741 ccaagtaaag ttgaactaac aagtttatca acaacttatc aaaatcttgg aactaataat
    43801 aaagctgata attcaaaaag taataagact acaacagata aatcaagtaa caatgaaaca
    43861 aaatcaagta cgaaaagtga tagtaatgga gataaaacag ataatgattt aaatgaagat
    43921 aagactacaa atgtgtcaga agcattaact ggaaatagtg agactaatag taataacacg
    43981 gataaagatg ataaaactcc attgtatgat aagataccaa atgttataaa tatagaagga
    44041 aatgctaaag attcctctta tgtaggtcaa tttttatata aattaaaggg attatactat
    44101 ttcaaggatg ttaaattaca tagcataaaa agtaatgcag aagattctaa ctatacattt
    44161 agtatgacat tatacctgaa agaaggtgta ttaactaatg aataaagata tatttaaaaa
    44221 agatataaaa gatatattta aaatggaagt tagtctaaaa gggattctta taaaggttat
    44281 aattttatta gctattttat catatggtta ttttggactt taccctcaat atcagaaata
    44341 tattgctaca aaaaattctg taaaacaggc tactgaaaaa ttaaatatat ataaagagaa
    44401 aactgcactt atgccaaaat atgaaaaaca acttgaagaa gttgaaagtg aattaaaaga
    44461 aaaaagcaaa aatcttttat atgatatgca agatggtctt tttttaatag gtttagataa
    44521 aaaaatgaag gcttataata ttgacttaac cagttatgac gttggaaatg ttaaagaata
    44581 cgaacatttt tatggtatat caacaacaat aagtgttaga ggtgattata gaaatgtcaa
    44641 aaaattaatg gcttatttag aagaacaaaa aaatgttact caaatactag attatacaat
    44701 gacttcatat atagaaccag aaaaagaaga ggaagaaaca acaaacaata aggatttaat
    44761 taaaaatgtg aacataaata aagatacaaa aatatactgg gtggaaggcg aaaataagtg
    44821 gcatttatat aaggattgtc cattgataaa agagggagag attataaaaa atgacaaact
    44881 tttagatagg tctactaatt atctatgtaa tacttgtgta cttagagctg caacagaaaa
    44941 agaagcaaaa aaagaagtcg tagctccaaa ggcagaaggt gtagtagatg ctacatttaa
    45001 atttataatg tacactaaag agaatactac aatgatactt gatacagata atccatcttc
    45061 atggaggcca ggaaaatata atccatttaa gtctagtgcg aaataaagct aagtaagctt
    45121 taaagatagg tgatgagttt gtacaaaaat aatgaaaaag gtcttacttt attagaagta
    45181 ataatagctg tttttatact gacaatagtt ttaagtattt cttataaagt gttcaatggt
    45241 ataacatcag cagtaaaaaa gcaacagatt attacagatg ctcaagtaaa tattaatcta
    45301 attaataagt atctaaatag agatttggaa aactgtaaag aactaactaa aactggttca
    45361 ggtaataatt atgaatacaa tatagagatg ccagataatg ttgtaaaata tgaagtttct
    45421 atagaaacta aaaaaaatac agaggtatat tctgtaacga gaatccaaaa caatacaatt
    45481 gatacagaaa atgaagttag agaagaaata atctataatc aaccactagt tcaaaacaat
    45541 aaagaaatga aggaaacacc atttaaaata gaaaagcaaa ctggtaaatc tatttatact
    45601 gtaagtatat actataatga atcagtgcaa gaaagtcata aaaatagcaa tttaaataac
    45661 aaaacttata catttgatgt tatgtcaaga atagggtaat aagaatttat taaaaaataa
    45721 gtaggttgat aaaatatgag taaaaagaat agtgaaagag gattttcctt aattgaagtg
    45781 ttagtagcta tggctattat gggaattgta ctatttgcat tttttaatat tatcaatact
    45841 aataacaaag caaacactaa aaatgacaca gatataactt ccttaaacta tgttcaaagt
    45901 gaaatagaaa atctaagaga aaagataaaa agtggagaat ttgattttga tagtttagat
    45961 aaactagaag atggaactgt cgtatatgaa aaattaatag ataaatcaaa aaaagttgta
    46021 tatgataaag ttcttagtga aggtgatgtg agcttatatg atactccata tgaaaagatt
    46081 acaacaataa aagatgaaga tggtaattta atcgacaaag gaaatattac aaataagata
    46141 aagactattg tagaagataa aagtggtcag atatataaaa tagctgtaac tgggaaaagc
    46201 atgaatgact attcaagtaa aaaagaggtt aaaattgtaa ctgaaatatt taaagataaa
    46261 taattttact tgggggtgag attttgagga agtggaataa atttaaaagt gaaagagggg
    46321 cagctttagt tttagttttg gttgtagtag cattgctttc tattgttgga ttgatatttt
    46381 caaatcagat agctaataga ataaaatcta caaagactac taatgaaggt atacaagcaa
    46441 agtatttagc agagacatgt gtggaaaata gtattgataa agcatatgaa aaattgtatg
    46501 atgagcttga gaaaatggat aatgagttta aaagtgaaaa tcaagaaaaa tccatatcta
    46561 ggtcaaaatt aagaaatata tcagatgaag attttaataa tcaagatgag gaaaatattg
    46621 aagcagaaag acttggatat atgaataata taaatttcta tttaaataaa gcttcttcag
    46681 acttggaaaa agctagtatg gaattgaaaa agctatatga tttagatatg ttggactata
    46741 gagacattga atatgtagat gcaaatatta tttcacatag agattctatc ttagaaattt
    46801 gcaagaatta tacttctgga gatattagta aaataaatga atatattctg aaagaagata
    46861 tagactcaac tacattgatt gaggcaaagt tagtgaataa tgatattctt ttaaaaatgt
    46921 ttttagaaga aaataaaatt gaaaatgaac atttaaattc agccttttca catacttata
    46981 aggcattaga taacatttct ttggcgatgc aaaatatgat tgagtataga catacgtttc
    47041 atatagatga gccaaaagta gaagtatcta atgggattcc agactctcaa caatattatg
    47101 aactaataca aaatcctata ataaatagta tggagtatag ctggaatagt aaatgggata
    47161 cattagaaaa cctattggaa attttaccaa atcaaactca aggatttaat tctttaagag
    47221 ttcatttgag aaataatgta agaaaatttg agaagttaag cgataatata tctagtggta
    47281 aaaaaaatac agcaaaaaat ttcttaaagt ataaagagtt attgtatgaa atatcagacc
    47341 aatgtaatca actaaaaagt atgtcttatg agaaaatacc tgttaaatat gataacatgg
    47401 ctttgataac aacttttgac tatatacaaa atgagctttt agcagagata aaatgcagat
    47461 taaaagaatt aaaaccacaa gaaattgata aaacagaagg tattactata aaaatacctt
    47521 tttataaggc tgactacgac atgacaaaag aaggatggcc taaattaaaa gaaaatggtt
    47581 caggggcgga gttaagctta atggtaactg gtgataaaaa tggaataaaa gaagtagaag
    47641 ttacagatgg taaaaaaaat attattggtt taggtgttga agaaaattca aattcaaagt
    47701 ataaggttga cgccattgta aactttaatc taaatataga tacaaatgta gttggaaatt
    47761 atgatataaa agataaaatt ttgattaatc atgatataag ttcatacaaa aaagtaaact
    47821 aaatataaga agcaacttag tataaataaa taataaaata attttattat ttactactaa
    47881 aaaaataagt aaactaatag aagatatgga gagtaaatat tatgtatatt tatgatttat
    47941 tagagcaagg tataagacta aacgcatcag atatacacat aacagttgga acaaatcctg
    48001 tagctagagt aaaaggtgga tttgtaaaac taagtgaaca aatattgaca tcagaagtaa
    48061 caatgcaaat ggcaaaagac atagcagggg aatctatgtt taaagtaata gaagaacatg
    48121 gagaagctga tttttcagct tctctaaaaa caggagaaag atttagggta aatgcatata
    48181 ggcaaaaagg aaattacgca atagcaataa ggactataac agcagaaatt ccaacatttg
    48241 aaaaattagg ccttccagag agtataaaat cttttactga aaagcataaa ggattagttt
    48301 tagttacagg tccaacagga agtggtaaaa gtacaacact tgcaagtatg ataaatataa
    48361 taaatgaaaa acaacaaaaa catataatta cacttgaaga cccgatagaa tatgtacatc
    48421 accataaaca aagtttagta aatcaaagag aagtaggaac tgatacagaa agttttcatt
    48481 cagcccttag agctattttg cgtcaggacc cagatgtaat actaatagga gagatgagag
    48541 accctgaaac tgtttcaata gcattaacag cagcagaaac aggtcattta gtattttcaa
    48601 cactacatac agtgggggca gctaagacaa ttgatagaat agtagacatg tttcaaccat
    48661 cccaacagca acaaataaaa acacagttat ctacagtttg tgaaggtgtg gtatcacagc
    48721 agttacttcc tacagcagat gggaaaggca gaatagcagc aattgaattg atgtttgcta
    48781 ctccagcaat aaaaaacctg ataagggaag gcaaaacata tcaaattcca aacatgatac
    48841 aaactggagt taaagtaggt atgaaaacta tggaccaaga tttaatggaa ctttataaaa
    48901 atggaaagat tacaaaagat atggcactta gtaggtgtac tgaccaagag tttatgacaa
    48961 gaatgatagg tggagtaaac tacaatggat attataatcg ttaatctata tgtatttata
    49021 gtgggtatag tgtttggaag tttctttaac gtctgtattt ttaggattcc agaaggtata
    49081 tctatttcta gaccaccttc tcattgtatg agttgtaata ctagactaaa gccaatagac
    49141 ttaataccag tgctaagcta tttaatgagt ggcagaaagt gtagatattg ttatgagaaa
    49201 atttctagta gatatgctat agtggaatta attacaggac ttttattttt aacagtattt
    49261 atgttatatg gaatatctac atcaactatt tattatttgg ttcttgtgtc tttactgatt
    49321 ataattacat ttatagatat tgaccacttt ataataccag ataaaatatt ggtttttgga
    49381 gctatatttt ctatgatttt taacctttta tttaaagaaa ttccagttaa ggattcaatt
    49441 ctaggtgttt ttatttgtgg aggaagtgtc tggattatag ttcttttgat tgaatttgta
    49501 ataaaaaaag agtgtatggg tggaggagat atcaaactat ttgcaatgct aggactttac
    49561 atgggtgtta aaaatggtct tttgacagcc ttactaagcg tttatgtagg agcagtctat
    49621 ggaatctgtg taataattat aagtagaata aaaggaaagg aatataattc tgttatacca
    49681 tatggacctt ttatatctat tggagcatta ataacaatct tatctggaaa gcaattactt
    49741 gaactttatt tttctatggt tatatagatg atagtcaagt taaataaagt tttaatttaa
    49801 aaaaatgaat taaagtattt aataaggaca atattattat taaccctatt actaaagtgc
    49861 tacaaactat ggagtatata ataataattt ccttattttt tgcacatttg ataaataaat
    49921 ctctatgtgt tttttgtgat agagaaaaga aaaatttata taatacgttt tttatttaca
    49981 taataactgt agttttatct gtattaatat atctaaaatt tgggattagt gtagaatgtg
    50041 tacaatatat ggttttaatt ccatttttaa taattatatc cattgtagat tactacacaa
    50101 tatatgtata tgatataaca atagttagtg gtataatagt tcaaggtata atttttttgg
    50161 cctttttcaa tatagctcaa tttgattata taaatcatat atttggacta ttgataggct
    50221 ttattatacc ttatattatt gctaaactaa caaaaggatt aggctatgga gatgtaggtc
    50281 tctatagctt atgttgcttt gcattgggaa ataattatgg gttttatgtg atagttttat
    50341 catttatttt agcgtttttg ttttttatta ttttatacat aactaaagta tttaaagatg
    50401 tagatataag aaagcaaatt ccttttacac cttttatttc cttgtcaaca attttaataa
    50461 tatttacaga gtataatata tttaattttt attataattt gttatacaaa tttatataac
    50521 aggaggtaag aaatgtacgt agtagtaggg ttaggtaatc caggaaagaa gtatgagaag
    50581 actagacata acgttggatt tgatgttata gatattcttg ctaaagaata caatattagt
    50641 gttactaaaa taaagcataa agctttgatt ggtgaaggga gagtaggtac tgaaaaagta
    50701 ttattagtta aaccacaaac ttatatgaat ttaagtggag aaacattaat agatatatat
    50761 aaatattata aggttgattt aagtaatata gtagtagtat atgatgatat agatttagaa
    50821 gttggaaaga tacgtataag aaaaaaagga agtggaggaa ctcataatgg catgaaatct
    50881 ataactaagt gcctaggttc aaatgacttt ccaagagtga gagtaggtgt atctaaacca
    50941 gaagctggtc aagacttagc agactttgta ctttctagat ttagaaaaga agaatctgat
    51001 aacataaatg aagcattaga aaaagcggca gatgctattg actcaataat aagagaaaat
    51061 atagatatgt ctatgaacaa atataatgga tagtgagagg gagatttaaa tgaatgatgt
    51121 ttttttatat cctttgcaaa attcaaaaga gtataaagat ataataaact gtataaaaaa
    51181 tacaaaaggc tcattattgg ttaatggact tttaccagta caaaaacctc atatatcgta
    51241 ttctatattt aatgatttat cacgacaaat gatttttata acgagtagtg atttagaagc
    51301 aaagaaagta tacgaagatt taagttttta tatggaagat aaagttgaat atttaggttt
    51361 tcaagatatt tatttttatc acttggatgc aaaggacaga aacgaagaag caaagaaatt
    51421 aaaagtattg ttaaaactag caaataaaga aaaaatcatt ttagtaactt ctattgaagc
    51481 agttttaaga aaatacatac caaaacaagt tttgctcgat agtgtatctc attataaggt
    51541 gggagatagc ttagacttag aaaagttaac tgagaaatta gtttcattgg gatatgaaag
    51601 agtttcaaaa atagaggggt ttggtcaatt tagtataaga ggtggaatta ttgatgtatt
    51661 ttcacttgaa tatacaaatc caattcgtat ggaattattt gatgatgaga tagattccat
    51721 acgaactttt gatgtatatt cacaaaaatc aattgataaa ttgcaacaat tctctataac
    51781 tccatctagg gaatttattt atcccgaaaa aactacagat gcattagtta aattaaaaaa
    51841 agaaacaact aaaaatactg atgaaggtac atttcaaaat atagattata tatcaagtaa
    51901 gacttatttt gaaggtgtag agaactatat agattatatt tatccagaag aaaacaaaag
    51961 tatatttact tatttagcag atgatgcaat agtatttatc aatgatataa cgagacttaa
    52021 agaaagatgc gaaaattata taaatgagtt tagagaaaat tataaattaa atcttgaaag
    52081 aggtctggct attaaaaacc aaggtaaatt gttatatcac tatacagatt tagagtactt
    52141 agtaaaagac aagtctgtgg tattaaactc attattgcca aagtctataa ataacttttc
    52201 tataaaatct ataataaatt ttgaaagtag agaagttcct acatttaatg gaaaggtaga
    52261 tttgcttgta gaagaattaa atcggctgaa atataatgga tacaaaataa ttttagcaac
    52321 aaatacatta gaaagagcaa ataaattagg aaaagattta ttggacaaag gcttagaaac
    52381 gaccatttct aaagatagag atattgaaat taaatcttca caagttataa ttgtacctgc
    52441 acacataaac agtggttttc agtataaatc tataaagttt gttgttataa cagataacga
    52501 aatgataggt gtttataaaa gagcgtctaa gactagcaat aaaaaagtta aaaagggaaa
    52561 gaaaatagaa tcctttttag atttaagtgt tggagactat gtagtccatg aaaatagtgg
    52621 tgtaggtaga tacacaggga ttgaacaaat tacagtcaat gctattaaaa aagattatat
    52681 gaaaatagta tatcaaggtg gagataatct atatgtgcca atagaccaga tggacaaagt
    52741 acaaaaatat attggtgcag aagtagaaaa agttaagtta aataagcttg gaacaaatga
    52801 gtggacgaaa gcaaaagcaa aagttaaaaa agaaattgaa gatatgacaa aagacttgat
    52861 tgagctttat gcaaaacgtg aaaaaataca agggtataag ttttcaaaag atacaccttg
    52921 gcaggcagag tttgaatcgt tgtttccata tcaagaaaca gaagaccaat taaaggctat
    52981 agaagaaact aaaaaagata tggaatcaag taaggtaatg gataggctag tatgtggaga
    53041 tgttggttat ggaaaaacag aggtagctat tagaagtatt tttaaagctt gtatggacca
    53101 aaaacaagtt gctgtattgg ttccaacaac aatattggcc caacaacatt ataatacatt
    53161 caaagagagg tttgaaaatt atcctttgag agtagaagtt ttaagtaggt ttaaaacacc
    53221 aaaacaacaa aaacagatta ttgaagatgc taaaaaagga ttagtagata ttttaattgg
    53281 aacacataga ataatatcca aagatataaa tttaccaaac ttaggtcttg tagtcattga
    53341 tgaagaacaa agatttggag taaaacataa agaagcttta aagaaaatta aatctacagt
    53401 agatgtactt acactttcag caactcctat accaagaaca ttacatatgt ctttaagtgg
    53461 aataagagac atgagtgtta tagaagaacc tccacaagaa agacatcctg taataacata
    53521 tgttacagag agtaaggaaa gcgtaattca agatgagata gagagagaat taagtagagg
    53581 tggacaggta ttttttgtat acaatagagt tgaacacatt gaagaaatgg caagtatgat
    53641 tcaaaaactc gtaccagatg ctagagtggc tgttgcacat ggaagaatga cttctaaatc
    53701 attagaaaat ataatcctag gatttttaaa taaagattat gatgtactag tctgtactac
    53761 aataattgaa actggtatgg acatatcaaa tgcaaataca atgattatat atgatgcaga
    53821 taaaatggga ttggctcaac tttaccaatt gagaggtagg gttggaagaa gttcaagaca
    53881 aggatatgca tatcttttgt atgaaaaaga taaaacttta agtgagatag cggagaagag
    53941 attaaaagct atcagagaat ttacagagtt tggttctggt tttaaaatag caatgagaga
    54001 ccttgaaata agaggagcag gaaatatttt aggttctcaa caacatggtc atatggcagt
    54061 tataggttat gatttgtacg ttaaaatgct taatgatgca attaaaaaag tcaaaggtga
    54121 acctattgtg gaggaaattg atgttgaaat agatttaagt gtaaatgcat acattccaga
    54181 taattatatc aaagatgagt tgattaaaat tgaaatgtat aagaaaatag cttctattga
    54241 aaacaaagaa gacatgttgg atatacaaga agaacttgag gatagattct ctgatatacc
    54301 taaaccagtc cagacacttc ttacaatagc ttatataaaa tcgttatgta agatattaaa
    54361 aatagaaaag ataagacaat taaaagatga aatattatta gttccaataa caaagtatag
    54421 aactaaacag aaaataggtt ataatatagt tacagaatta gaagaattat tagaaaagat
    54481 gtgcaaggta aaataacttg taaatttaag aagggatgtt ttaaatttga aaaaagtaat
    54541 aacattggta atagctatga ttttggtagt atctgtgact gcttgcagtt ctagtaaagg
    54601 agaaactgtg gcaacagttg aaggtacaaa gatatcatca gatgaattta aaaaaactat
    54661 agctctttat aaagattcta tggaacaaac ttatggaaaa gatatatggg ataaggaagt
    54721 tgaaaagggt gtaaaataca aggacaagtt taaagattta attcttgacc aacttataac
    54781 tacagaagta atatattctc aagctaaaaa agataactta cttcctaaaa aagaggatgt
    54841 tgaaaaaagt tttaaagaat taaaagacgc tatgggtaaa gatgaaaaat ataaagaaca
    54901 acttaagaaa ttaggtatag atgatgagtt tttaagagac caacaagaga aagatttagc
    54961 aatgcaaaac tatcaaagta actttgctaa aaagactaaa atatcagatg aagaaatgaa
    55021 aaaatactat gatacacata aagatgaatt taaaaaagat gaggtagaag cttctcatat
    55081 tttattgaaa acagtagatg ataataacaa gccactttca gataaggaaa aagctgaagc
    55141 taagaaaaag gctgaagagg ctcttaaaga agttaaatca ggtgaggatt ttgcaaaagt
    55201 agcaaaaaaa tattcacaag atacttcagc aagtgatggt ggtaaattag gattcttttc
    55261 aaggggtcaa atggttgctg aatttgaaga tgctgctttt tctatgaaaa agggtgaagt
    55321 atctgattta gttgaaactc aatatggata ccacataatt aaagtgacag acagaataaa
    55381 tgagcaaaca tcatttgaag atgctaaaga aactataaaa gaccaactgt tgaaaaataa
    55441 atatcaagaa caaatagaaa aattaactaa agaagctaag gtagaaaaag atgaaaaagt
    55501 aataaataaa atcacaatct aagacttaaa aaaagagtaa tatgcttaaa aaagctatat
    55561 ctggataaga tatagctttt tttgtatatt tttcttaaca atggtaaaaa taaacttaaa
    55621 gtaatttatg gagggattaa aaaatatgaa agcaacaggt atagttagaa gaatagatga
    55681 tcttggaagg gtagttatcc caaaagagat aagaaagaca ttaagaatta gagaaggaga
    55741 ccctttagag atattcacag caaaagatgg agaagtcata cttaaaaaat actctccaat
    55801 aggtgaatta aatgaatttt cgcaagaata tgctgaaaca ttagctgaag tactaggata
    55861 tggagtgttg ataacagact tagattctat aatagctgtg tctaagcttc ctaaaaaaga
    55921 ttataaagaa aaaagtataa gtgatgagtt agaagcaata ataagtgaaa gaacagtaaa
    55981 atattcaaaa gataaaaaca ttttaccatt attcaaggaa gaccaaaaag aatatactag
    56041 tcaagttatc atgcctataa ttagtgcatc tggtgactgt ataggttcaa ttgtagttgt
    56101 ttcaaaggat aaagatacaa ttgcagaaag tgaagaaaaa tctttaaaaa ttgctgctaa
    56161 ctttttagga aaacaagttc aataatttta tcataaagac cttttacatg aaggtctttt
    56221 gaagtttttg tgtaaaatat tgaatatagc attaaaaata tggtatatta aagtttgtta
    56281 tataaacagt atggaggtta tgttatgaac aataacaaaa ataaagactc ctttctaaaa
    56341 ggagccctta tacttgggct agcaggtgta atcgtaaaaa taatgggtgg attttttagg
    56401 attccccttg gaaacatgat tggttcagaa ggaatgggat actatcaggc tgcttatcct
    56461 gtgtatactc tatttttaac attagctact gctggatttc caacagctgt ggcaaaattg
    56521 gtttcggaaa aagttgcaat tggaaatttt aaaggtgcaa ataagatatt taaggtatca
    56581 catacagttt tattcattac cggtataata tcattttgta tattattttt tggagccgac
    56641 tacatagtaa caaacgtaat gaaaaatcca ggtgcgttat actctatgaa agccattgct
    56701 ccagcattac tatttgttcc tgcaatgtct gcatatagag gatattttca agggagacaa
    56761 gatatgacta agattgctgt gtctcaagtt gcagagcaat tttttagagt tgtattaggt
    56821 cttactttgg cctacttgct tatgaagagt ctaggtcaga cttttggtgc agcaggtgct
    56881 atatcagggg ctacaatagg ttcaatagca tctatgcttt atttggtgtt tgcttatatg
    56941 ctagggagaa aagagcgtag agcagagata gatgcaagcc aaagatttaa agatgaaaga
    57001 gtgtcttata tttttaaaaa gcttttaact gttgccattc ctataacaat aggagcatca
    57061 gtaatgccac ttgtaaatat gatagataat gttattgtaa taagaagact tatggaagca
    57121 ggctttacat acaaagtggc aaattcaatg tttggtcaat taacaggtat ggcaatggcc
    57181 acaataaatt taccagcagt cataacaact gcaatgagta tgagcttagt tccagcaatt
    57241 tctaaagcat atgcattagg aaataagtca aaagctagaa aagatactaa gagtgcagtt
    57301 aaggtaactt tacttatagt attgccttgt gcgtttggta tggcttcttt agcaatacca
    57361 ataatgggtc ttttatttcc acatgaacca tcttctgtag gaactatttt gtttactctt
    57421 actccatgtg tattattttt aggattaata cagacattga ctggtataat tcagggaatg
    57481 ggaaaaccaa tagtacctgt aattgcttta tgtgttggta tgctatgtaa aattgtaata
    57541 agttatactt taacaggtat accagatatt aatgtactgg gttcagcatt tggtacagtt
    57601 acagcatact ttgtggcagc catgattaac ttactatatg ttaaaaaaca catgaatgta
    57661 aacttttcta aaaaagaatt tataataaag ccatttataa cagttatgac aatgtttata
    57721 atggttaagt tgagttatgg tgctttagtt ggattcttag gaaacagcat atctacaata
    57781 attgcaatat gtgttggtgg aattgtatat gtagtagtaa tacttggaat aggtggaatt
    57841 aaaaaagaag agattctaac tatgccaaaa ggtgataaat tatataaatt gttaaaaaaa
    57901 gttaaactta taagataata taggaatata tatgcaaata aaggaaatac tatacaaata
    57961 gtaaatctcc ttatttgtat aataatataa aaataaagtg aaatttgcta ataggagtga
    58021 tgttatgggt aagataagta ttgtaggtct tggaccagga gactattctc ttataagcca
    58081 aggtgtgtta gaatctttaa taaatagccc taaagttttt tttagaacag agaaacatcc
    58141 aataattgat aaacttaaag aaactataca atacacttct ttagactact tttatgaaaa
    58201 agaggaaaat tttgataatg tttatttgaa aatatctgaa tttatcatag aagaatcagc
    58261 aaagggtgac ctagtgtatg ctgttcctgg tcatccaaga gttgcagaga agactgtaag
    58321 tataatagag agattagcca atgaaaagca catagcagta gaaatattgc cttctatgag
    58381 ctttgtagac gctatgttta actatttggc tatagaccca tctgagggat ttaaactggt
    58441 agatgctttt gaaatagaaa attcatatat agacttggat acaagcatga taataactca
    58501 ggtatatgat aaatttatag catctaatgt aaagttaaaa cttatggaac attatgacta
    58561 taatcaagaa gtatgtatag taaatggagc aggtgtcaaa gatttagaaa gtaaaaagtt
    58621 tataaagctt tgtgaattag acagaaatga aaactttttt gactatttaa ctagcttata
    58681 tatacccaaa agttctaaaa agatgtataa tacagtacat gacttagaaa taatagttaa
    58741 tacacttaga agtccatctg gatgtgaatg ggataaaaaa caaacacatc aatcattaaa
    58801 aaattctgtt atagaggaag catatgaact ttgtaatgca attgataata atgatataga
    58861 tgaaatggta gaagagttag gtgatgttct tttacaggtt atattccatt gtcagatagg
    58921 aaatgaggag ggctactttg atttaaaaga agtagtaaat ggcatatgta aaaaattaat
    58981 tcatagacat cctcatatat ttagtggtgc aaaattagat atgtctaagt ttgagaaaac
    59041 ttgggaagaa ttaaagaaag aagagaaagg cgaaagtact ataacagatg gccttagaag
    59101 gataccaaaa catttaccag ctcttatgaa ggctagtaaa gttcaacata aagcagcact
    59161 tgtcggcttt gattgggaca atgtagaaga tgtatttaaa aaaattgaag aagaatatca
    59221 ggaattactt gacgaacaca gggtcgggaa tataaaatac ataaaggaag agctaggcga
    59281 tttattattt tcgatagtaa atctagctag atttttagac atagactcag gagaagcctt
    59341 gaactgtact acagaaaaat tcataaatag atttgacttt atagaaaaaa gtgcaatgaa
    59401 cttgaataaa aaattagaag acatgacact tgaagagatg gatgagtttt ggaatcaggc
    59461 taaaaaacaa taaaattgtg gaaggaaatt tgtcaaaaat ctagaacttt tatcacaaag
    59521 atagaagtct atattataaa catttttagg aggtaaatat cgtgaataaa gctgaattag
    59581 tatcaaagat ggcagaaaaa agtggattaa caaagaagga agcagaagct gcgttaaacg
    59641 catttatgag ttctgttcaa gatgcactag taaataatga aaaagttcaa ttagttggat
    59701 ttggaacatt tgagacaaga gaaagagctg ctagacaagg aagaaatcca agagatccag
    59761 agcaagttat agatatacca gcttctaaag caccagtttt caaagctgga aaaggattaa
    59821 aggatataat aaatggataa ttagaaatct tgtgaaaaag tgtggataaa tttgccacac
    59881 tttttctata gaaccaagga aggtgataat tatgagatta gataaatttt taaaagtatc
    59941 taggattata aaaagaagaa ctgttgctaa agatgcatgt gataaaggga ttgtcactat
    60001 aaatggaaaa gttgctaaat cttcttcaga ggtgaatatt ggagatacat tagaaataca
    60061 atttggagag aagaagatga agtttaagat aaatgaagta aaagagcatg tattaaaaaa
    60121 tgatgcaaaa gaaatgtacg aaattttaga ataaaattaa tagtcctcta gtatattaat
    60181 agtagtatta ttgaaatatt tactgggggg atattatgga acagaacata actttgaaag
    60241 atagatcaaa gctggttatt tcgggagtcg agcacatata ctcttttaat gacaaaagag
    60301 tagagctcaa aacttctgta ggcgagatgg taattgaagg cgaaaaccta gatatgagca
    60361 agctaagtat agacgaaaat ataattagca tagatggaac tataaattct atggtatatg
    60421 caaagcctaa aaagccacaa gagagtttct tgaaaaaggt atttagatag atgatacctt
    60481 ttactcaaga tgtaagcatt ttttatgcta ctatttatgg tggaatactc ataggggtgt
    60541 tgtttgattt ttatagaggg ctaagaggga attttaaatt catcaattat tttgctataa
    60601 tctttgatgt attgttttgg tttctagcta cggttataat attcgtaaca ataaacctta
    60661 cagaattttt tgatttaaga tattatcatt ttgtggcttt gtttattggt tttattttat
    60721 actataacac cataagtaaa attgttttat caattatcaa taaaataatt cgttttgtga
    60781 gaaattcttt taaaaaagtt acacattaca tagtcagttt tttaaataat ttgtattacg
    60841 ttataatcta t
//
LOCUS       DAKBNN010000005        49268 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000005 DAKBNN010000000
VERSION     DAKBNN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.005"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(354..866)
                     /locus_tag="OUB02_000310"
     CDS             complement(354..866)
                     /locus_tag="OUB02_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884124.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            1419..2303
                     /locus_tag="OUB02_000311"
     CDS             1419..2303
                     /locus_tag="OUB02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HCT8884125.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNNKLIYSNMIDK
                     KTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVP
                     YNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNK
                     DAKFSTKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFL
                     RYPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSE
                     GEIELPDPIIMENDKLMK"
     gene            complement(2616..3395)
                     /gene="hisJ"
                     /locus_tag="OUB02_000312"
     CDS             complement(2616..3395)
                     /gene="hisJ"
                     /locus_tag="OUB02_000312"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HCT8884126.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(3965..4315)
                     /locus_tag="OUB02_000313"
     CDS             complement(3965..4315)
                     /locus_tag="OUB02_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HCT8884127.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(4331..4747)
                     /locus_tag="OUB02_000314"
     CDS             complement(4331..4747)
                     /locus_tag="OUB02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HCT8884128.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(4766..4957)
                     /locus_tag="OUB02_000315"
     CDS             complement(4766..4957)
                     /locus_tag="OUB02_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HCT8884129.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(5108..5923)
                     /locus_tag="OUB02_000316"
     CDS             complement(5108..5923)
                     /locus_tag="OUB02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCT8884130.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(5970..6908)
                     /locus_tag="OUB02_000317"
     CDS             complement(5970..6908)
                     /locus_tag="OUB02_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8884131.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGRIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVMISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(6905..7777)
                     /locus_tag="OUB02_000318"
     CDS             complement(6905..7777)
                     /locus_tag="OUB02_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8884132.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(7802..9730)
                     /locus_tag="OUB02_000319"
     CDS             complement(7802..9730)
                     /locus_tag="OUB02_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HCT8884133.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(9842..10219)
                     /gene="gcvH"
                     /locus_tag="OUB02_000320"
     CDS             complement(9842..10219)
                     /gene="gcvH"
                     /locus_tag="OUB02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCT8884134.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(10306..12432)
                     /gene="cdhC"
                     /locus_tag="OUB02_000321"
     CDS             complement(10306..12432)
                     /gene="cdhC"
                     /locus_tag="OUB02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HCT8884135.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(12489..13295)
                     /locus_tag="OUB02_000322"
     CDS             complement(12489..13295)
                     /locus_tag="OUB02_000322"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCT8884136.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(13345..14712)
                     /locus_tag="OUB02_000323"
     CDS             complement(13345..14712)
                     /locus_tag="OUB02_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HCT8884137.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(14743..15687)
                     /locus_tag="OUB02_000324"
     CDS             complement(14743..15687)
                     /locus_tag="OUB02_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HCT8884138.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(15707..16477)
                     /locus_tag="OUB02_000325"
     CDS             complement(15707..16477)
                     /locus_tag="OUB02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8884139.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(16533..17918)
                     /gene="lpdA"
                     /locus_tag="OUB02_000326"
     CDS             complement(16533..17918)
                     /gene="lpdA"
                     /locus_tag="OUB02_000326"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCT8884140.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(17962..18843)
                     /locus_tag="OUB02_000327"
     CDS             complement(17962..18843)
                     /locus_tag="OUB02_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCT8884141.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(18879..19553)
                     /locus_tag="OUB02_000328"
     CDS             complement(18879..19553)
                     /locus_tag="OUB02_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HCT8884142.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(19590..20462)
                     /locus_tag="OUB02_000329"
     CDS             complement(19590..20462)
                     /locus_tag="OUB02_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HCT8884143.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(20554..21186)
                     /locus_tag="OUB02_000330"
     CDS             complement(20554..21186)
                     /locus_tag="OUB02_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HCT8884144.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(21291..22967)
                     /locus_tag="OUB02_000331"
     CDS             complement(21291..22967)
                     /locus_tag="OUB02_000331"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCT8884145.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(23216..23995)
                     /locus_tag="OUB02_000332"
     CDS             complement(23216..23995)
                     /locus_tag="OUB02_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8884146.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(24305..26224)
                     /gene="cooS"
                     /locus_tag="OUB02_000333"
     CDS             complement(24305..26224)
                     /gene="cooS"
                     /locus_tag="OUB02_000333"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HCT8884147.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(26831..27742)
                     /locus_tag="OUB02_000334"
     CDS             complement(26831..27742)
                     /locus_tag="OUB02_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HCT8884148.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(27858..28436)
                     /locus_tag="OUB02_000335"
     CDS             complement(27858..28436)
                     /locus_tag="OUB02_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCT8884149.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            28662..29585
                     /locus_tag="OUB02_000336"
     CDS             28662..29585
                     /locus_tag="OUB02_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8884150.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(29757..30899)
                     /locus_tag="OUB02_000337"
     CDS             complement(29757..30899)
                     /locus_tag="OUB02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCT8884151.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMDEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(30917..32671)
                     /locus_tag="OUB02_000338"
     CDS             complement(30917..32671)
                     /locus_tag="OUB02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HCT8884152.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            32807..33013
                     /locus_tag="OUB02_000339"
     CDS             32807..33013
                     /locus_tag="OUB02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884153.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(33018..33941)
                     /locus_tag="OUB02_000340"
     CDS             complement(33018..33941)
                     /locus_tag="OUB02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884154.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(34194..35894)
                     /locus_tag="OUB02_000341"
     CDS             complement(34194..35894)
                     /locus_tag="OUB02_000341"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCT8884155.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(36306..36719)
                     /locus_tag="OUB02_000342"
     CDS             complement(36306..36719)
                     /locus_tag="OUB02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCT8884156.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(36785..39163)
                     /locus_tag="OUB02_000343"
     CDS             complement(36785..39163)
                     /locus_tag="OUB02_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCT8884157.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAKKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            39482..40933
                     /locus_tag="OUB02_000344"
     CDS             39482..40933
                     /locus_tag="OUB02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HCT8884158.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(41137..43650)
                     /locus_tag="OUB02_000345"
     CDS             complement(41137..43650)
                     /locus_tag="OUB02_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCT8884159.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFCFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(43873..44694)
                     /locus_tag="OUB02_000346"
     CDS             complement(43873..44694)
                     /locus_tag="OUB02_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8884160.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(45212..45688)
                     /locus_tag="OUB02_000347"
     CDS             complement(45212..45688)
                     /locus_tag="OUB02_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HCT8884161.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(45722..46300)
                     /locus_tag="OUB02_000348"
     CDS             complement(45722..46300)
                     /locus_tag="OUB02_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT8884162.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(46404..49004)
                     /locus_tag="OUB02_000349"
     CDS             complement(46404..49004)
                     /locus_tag="OUB02_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCT8884163.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLDTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTNKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        15105 a         9386 c         5479 g        19298 t
ORIGIN      
        1 catttaatat tcttgacaca attattctta cattctatta ttgtttattt ttgacgcaat
       61 cgttttttat tcagtcattt aatactttcg acacaatcat ttttacattc tattattgtt
      121 tgttttgata caactgtttt tattcagtca ttttatattt tcgacataat tattcttaca
      181 ttctattatt gtttattttt gacgcaatcg ttttttattc agtcatttta tattttcgac
      241 acaatcattc ttacattcta ttattgtttg tttttgatac aactgttttt attcagtcat
      301 ttaatattct caacacaatt accttttcat tcaatcaaag taatctttaa gcattatttt
      361 tcaagctcat caaagtaatt atatacgata acctcacctg tctctttatc tgtagtcttt
      421 ataatgccct tagctccatt tacctcttct tttgtaaatt catcatctag cacaactttg
      481 aatttatctt cctttccata accttgttga aattcatctc tcaaaattgc atttttttct
      541 gcttttggct tcaactcaaa ttcaattcca gatttcattt catcaaaata atttaatact
      601 acaacctcat tattcatttt attagttgtc tttataatcc ctttagctcc atttacttct
      661 tcttttgtaa attcatcatc caatgttata ttaaatttgt cttcttttcc atattcctct
      721 tgaaaatccc tcttcatttc attgtattta gtttcaaact ctttactctt gtttaattcc
      781 atagctgata catttgtcaa tgatgctcca attgccatta ctgttaatgc cattactata
      841 ccacttttaa atttttttac tttcatatct tacctcctaa attaaatgtt ctcaatgaaa
      901 ttatataaag aaaatcctaa gttttacagt ttcattcttg taactaaaat tactattatg
      961 taaccaaact ttttgcttca actattcctt aaaagttgct tttttgagga atagttgaag
     1021 ttaaataatt agattttatc tatatttata gatttaaaat caaatttatc catataaata
     1081 aatacttatt tttgcaaaat aaaaattttt aattactcca atccttctta tttacttaaa
     1141 atcctatgat aagaaattta aaagtcttta ccttctttaa caataattta tttactttta
     1201 acatacaaaa tatagagagt atatcaaaat cattttactg aatattctaa aaataagtac
     1261 cccctattag tatatatatt tacatattaa aaaaaagttt taaataaagt tatatttttt
     1321 acaacagttt tgtaattaaa aacatattga aggtaaattt aaaggtacta tatactataa
     1381 atatgtcgac acaataaatt taaaaaggag aactaaatat gaaaaaaaga ttaataataa
     1441 tgatattgtc tgttgtctta gtactatcaa gtatacttac gatatttgca tattcaaata
     1501 ttaaatataa taataataat aaattgatat attctaatat gatagataaa aaaactcaaa
     1561 atagtgtaaa ggaaatccta aaagaaaata aaattaatga aaaagacata gatacattta
     1621 ttaaagcagt caacaattat aataaattgc aagttaaaat acttcaaaat aatataaata
     1681 tatctaaatc tggatattct tcaatcaatg caaaacaagt tccatataac ctagaaaaat
     1741 tgcaagataa ttgggtgaaa aaattccctg attatatgga tgtaaattgt agaataactg
     1801 cttttagact ttttaaagat tttataaatt caaataagaa atttactgga gactctattg
     1861 acttgaatgt cgacttggac actataatga ataataaaga tgctaaattt agcaccaaag
     1921 atgtagaaaa atttatcaac ttcttttctg caataccagc taaagataca gatgatacaa
     1981 tcaaaatttc agaacaaata aaaaatgaat ggaaaaaaag aaaaatatct tttaaaaata
     2041 ataaaaatat atctattata aatggatttc ttcgttatcc agaaactaaa aacgtattta
     2101 taggtcatac aggcatatgc attaaaacta aaaatggcat tctattttta gaaaaatatg
     2161 gtgttacatc accttatcaa gttactaaat ttaaaaataa agaagatgta aaaaattata
     2221 tgtttaatag attgaagatg tcagaaggtg aaattgaatt acctgaccct ataataatgg
     2281 aaaacgataa acttatgaaa taataacttt aaacgcaaaa tagtatttat aattactaag
     2341 tcaatataat agagaaggtc tatagatttg tgtaaataca agcttatcta caatcttata
     2401 taagaatgtt tatataaata agatagatta atcctaagtc ctattattct attgaatttt
     2461 attattattt tatttagatt ctattgttat tttacttaga acttattatt attttattta
     2521 agttctatta ctattttcaa tcatataaat ttacaaaaga ctgtacataa atttatttta
     2581 tacaaactta tctacagtct tttgtgtgaa tatatctaaa taatatcgta tttatttatt
     2641 tcctctgcac agtgagaatc agaacctaaa actaatggaa tatcgtattt ttttaccaac
     2701 tctaataagt atccatctat ataaggctct ttgcagtact cttttctatt tccagaaaca
     2761 ttataatcaa gttcataacc tttttcttta attaacttta ttaactcttc aagaattgta
     2821 tttcctttat aatcgtatgg aaatacctga ttaaactttc taactaaatt taaatgacct
     2881 attctttttg gtttatgtat acctaaatct gaatttacag cttttatcaa tgtttcataa
     2941 tacttattat aaacagcttc aatacttcct aatttgtcga ttaaattttg aaactcctct
     3001 acactgtaat ctacacaata ataatcatca tcaatcttaa ttatatgtac tgataaaaga
     3061 gaatcctcta attcctttcc atatttattt aaattctcct ttatcccttc ttcgtatcct
     3121 tctataaaat ccacttccga cccaacattt atcttgattt taccctcata cttttctttc
     3181 aacttcttga catcattaaa atactttggt aaatcttcaa tactcataca actatcattt
     3241 tcaggagcag ggtctctaaa tccatttgca tacggaaaat gttctgtaaa tgtcatttct
     3301 gttattccaa catccattgc tctttgtaca tatttttcaa aatcatcctt acttccatga
     3361 ggacaataag gtgaatgtat atgaccatct ttcataaata attcactcct tttttaaaga
     3421 aaaaatctaa tataaatgca taaatctttt aacttcaaaa taatattttt aatcatgcta
     3481 atttttatta tatctaatat tagagtaatt ttaaactgcc ttttaagtat cttatcatta
     3541 gtactatttt caacttaaca ttaatattat tttttaactt aacattaata ttatttttta
     3601 acttaacatt aatactatct ttaaatagta atactcaata tataattact taacataaaa
     3661 taactcaaat tgcttgatga ggcttttgca gtatttaaag aatatataat tatttaacat
     3721 taaataatcc aatcttgtaa taaaatttac tcattaaagt atattacaga aaatacctca
     3781 aaataaactt tttagttcat gagatactct tttttacatg aaattagata tatttttatc
     3841 atttcaacaa tgaaagggag gcttatatcc atctttagac aaccccttag ataaaatttt
     3901 taataattaa acatcacaaa atataaaaat cgcttatatc aaacgtataa gcgattaatt
     3961 atatttacat atgtttttta gcctctaaca acattttatt agcttcttcc atatactcta
     4021 tagccttttc aatactcttt gctgtttctt cttttccaat agctctagcc ttttcaaccc
     4081 actctctaaa tccttcttca tgagtttcat tgtgatttat ccaatgtact aaaagtattt
     4141 ttaaagtttt ttcatcctta gactctgatg attcctcatg actatgttcg tggtcgtggt
     4201 cgtgagcatg gtcatgtgga tgttcatgac atatatctcc atgagtatgt tcgtggcagt
     4261 ggtcatggtc atgatgtcca tgatgatatc ttacaatctc tttatcaaaa atcattagtt
     4321 attccccctg ttattttata ttaaactctt tttttataag gtttacagaa tcttttacaa
     4381 gtagtgctaa tggtttacct tctaatccaa ctaactccac attttctggc ataataggta
     4441 aaagtatctt aagtgcctca ctgtctgcaa tagcttcact tatattacaa gtaacttctc
     4501 ccatcataga attaggtatt attacagaaa taggtgcaac tattatattt gcttttttag
     4561 ctgatactac tatagcattt tcccctgtag ctcctttatt agcatgagct ttcatcatag
     4621 aactagttgc tatagaatta gtccctagtg cataaatttc tacatatgta ggtaactctt
     4681 ctctcaaaga agaaactatc tgggctccaa tacctccacc cattccatct atcactgcta
     4741 ttatcatatg aaattcccct attaattaat tttcttgtat tattatctta tggtccatca
     4801 atcttatttc tttaattatc ccatctatta tcttttgctc tcctaataaa tccgttaaat
     4861 aaatctttcc atcacaaggg tctatattaa ccacattttc cataaccttt tctaactcat
     4921 tattactttt acaaatgaaa gctgatgatt cacacatata aatctcctcc taatatgatt
     4981 tttattaaaa atcaaaaaaa gattccctta ggtatttcaa taaaccaaag aaaccttcat
     5041 gatttcagtt tttgtatatt attatacctt aagtatatca ctttaattta tgtaaagtca
     5101 attaaagtta acgcttttta ttatactctt cattcttgta tttttcatat agttctttaa
     5161 ctaattgctt ctcataatca gtcaaatctc cctccacaaa ctcaacatta aattgttcag
     5221 ctaatccctt tactatgttt ttctgtagaa tatctatgtc tatttttctg ccaatttcat
     5281 tttctattcc acttgctttt ttagctgtaa atttcatagc tctttctttc aattctggcg
     5341 tcttagtttt tattaattta aacaattttt ctacatcaaa atccagtatt attgaaccat
     5401 gttgtaataa aacaccatct tttctgcttt gagcacttcc aattaccttc ttattattaa
     5461 tagtaacttc atatgatgca tgtgcattaa aacatgcagc tgataaattt tccctactta
     5521 ttctctcgcc tctatttaaa ttatctgttt ctataccact tagattcaat cctttaacta
     5581 gaccttcact tatatatcta taagataggt tgatgctctt atcgattaaa ggattatctt
     5641 ctcctataat tatactatag gttagctcat tatcatgtaa tacagctctt ccacctgtaa
     5701 ttcttctagt atagtctata ttcatacatc tacacttatc taaatctatc tcatcttcca
     5761 atttctgaaa atatcctata ctcaaacaag ctggttccca tgtataaaat ctaagagttg
     5821 gcttattatg tcctttctta tatgcagtaa atatagcttc gtcaattgcc atattcattg
     5881 ctccttcata tgatttatta tgtataactc tccactgatt catgattatt acgcccccat
     5941 ttttagtata aatatatcct agaaatcctc tatagattta tttctcttat tgctaaatct
     6001 atctcacttt gatttaaagg tcttgaatag ttgtatatag tacttcctgg tctttcatta
     6061 tagtatggtc tattacaatc cttacaacct cttatttcaa aaggatagcc tttgtttatg
     6121 tcttttaaaa tatcattgtc aagctttata ctatttaagt atccattttt aaacttaaaa
     6181 tactcttttg gataaccttt atttatcata taagacatta actgtaccct tctataactt
     6241 tctatgcttg gttgacttat cttttccatt ttagttcctc taactggagt aaatgcaaat
     6301 aaactaatca ttacatcatt ttcctttaaa taagtatata aattgtatat atcttcatga
     6361 ctttcaccca taccaactat tatatgtgta cttattttat tgggatatga ttttgacatt
     6421 tcagttataa attttaactt ttcatcatag ttatttcctt ttattttttc atatagttct
     6481 ttatttgctg catctattgc tattcctatt ttatcaactc ctactgaaaa aaacttcttt
     6541 atctgttcat cattttctaa ttttgcagat agtgaaattg gcatgtctat cctaccactt
     6601 atatagttta taaagtctaa aacagagtta tgtgcttctt cacttgccat tgattgaata
     6661 catattctct ttatattttt tcctttataa gcttttaaag catctaatat ttcttctttt
     6721 gaaaactctg gccaaattac tcttgataat ttatcttttc tagtactact ttctattgat
     6781 tgagaacaaa aactgcattt atttatgcat ttttctccaa tcattatata tgctgtggta
     6841 ggaggtatat cgcttttttt gtttaatatc ccaagttcta ttgctgtccc aactgatagt
     6901 ctaatcataa aacgcaacac tccttacact catttattgt catattcatt tctatagcct
     6961 tattctttgc agaccttgat ggtaatacta ttctattgac tccacacatt atagataatt
     7021 ggtctagctc ttttctatac actcctcttg gtctcataca acctagatta atttcagtat
     7081 ctggtaaatt aattctagct tcacataaaa tatctgctac cccttctaac tcaggaggtt
     7141 ctacattttc atactcagtt ccttttgttg gaataaggac tataaatgta agcttattag
     7201 gaggattttt ttgtaggtat tctataattt catattcacc ctttatttgt cctcctttta
     7261 aaccaataca tatatgtggt gctacttctg tatgttcttg tattgtatta tagacttcaa
     7321 tataatcttc tttactcttt ttcatcttat aaacttctct tattgtctca tcatcaaaca
     7381 ctaaatcaaa agaaactata tctaaatact tacacaactc tacaatgcta tctttatcca
     7441 taagacctaa atgagcattc aatctatatc cttgctcttt taagttttta atggtattaa
     7501 tatgagtatt tatagggaca tctccctcat aactacaacc gccactcaat agaaagctag
     7561 ttatattcct tgattgtact ttttcctcat attcttctat tggcaccata ttgtttaagt
     7621 agtgaccgtt gcaatgggca catttcaaac tacattgatt gcttgtagta ctaagagcta
     7681 gtgtctggtt aggataagca aactctattt tatttccaaa attctttaat ttaatatcaa
     7741 atgctatttt cattttttca gatagattct tattcatagt ttattccttc ttaagtacat
     7801 tttactcttc tagtaatttc tttgctgttg ggaactgctc tatattcgta tgtggtaaga
     7861 aacatgcaga aataaattca tccatataac ttggatatac actaagttca acataagtta
     7921 tttgattacc tatttcttca agtttatttt ttgcatcttt acttataaga gctaagtaag
     7981 aaccaacaag tgaactattc cctatataag taaatttttc tctatcgata tcaggaagta
     8041 accctataat tattgagttt tctatgtcta ggttgtttcc tataccacct gctatataaa
     8101 ccctatctaa tatactaaaa tccataccta aactatctac aagagtgtat gcgcctgaat
     8161 atatagctcc tttagctctg atgaaattat ctatatcaac ttcattgact actatatcat
     8221 tttctaaatc aaattcttct ttaaatgcta atacatactc tcctatctca tgctcattaa
     8281 atctaactct cttattatct aaatccctgt atattttacc tcttctatct attacacctt
     8341 tagtaatcat ttgacagatt aaatcaatta taccagaacc acatatacca actggtgcac
     8401 attcatcaat aatctttaat gttggctcta aagtatcttt atcaataatt actttctcta
     8461 ttgctcctgc tgatgctctc attccacagc ttatccctcc accttcaaaa gcaggtcctg
     8521 ctgaacatgc acagctcatc atataatctt tatttccaaa tacaatctct ccattagttc
     8581 ctaaatctat aaataaaaca ttttcttcac ttgaccatat ccctgctgat aatactcctg
     8641 ctgttatatc tcctcctaca taacttgcaa cagatggagc taagtacaca tatgcactgt
     8701 catttacaaa aagaccaaca ttttcaccca taagttttgg agattttaaa aatggtggta
     8761 tgtaaggttc ttgtcttaag aagtctgtat acacacccaa gaataaactt gtcatagttg
     8821 tattcccggc agccactaat gtaactacat cttccttatc tattccattt ctttcatata
     8881 ttgtttttaa tagtggattt atagtttcct caactatagc tttatgaagt gtctctaatc
     8941 catttttctt agttgaatat ataattctat ttataacatc tgctccatat tttatctgtg
     9001 catttccaga agatgcttta tctactacct cttttgagta taggtctact aaacaaacaa
     9061 ctactgaagt tgtaccaata tctattgcaa ctccgtataa ggaattttct ttatttcctt
     9121 gttctatatt tatgattgta agtttctttt gtttttgaac atatgttatt gtaaccttaa
     9181 aatcactctt tctaaataca gttggcattt ttctaagtat atccaatcta aaatctattt
     9241 cattatatcc taggttattt ctaacatatc tttccaatct atctacatca cttatattat
     9301 catctaaatt tggttcttcc atttctatgt atttcttttt tatatttgtt ttaaattgaa
     9361 ggttatgttc ttcaattatt tttttagctc tttcaaaaat ttccctatcc tcttttttat
     9421 tactaccttc tatcttcata ccatgcatag aagatgatac tttagaagga acttcaattt
     9481 ctatatctga tattaccttt gtacaacaag ctagtatata accttgctcc cactcatcat
     9541 ctgttatatg tcttgttttt tctgtatcaa cttttccatt caataatttt actttacact
     9601 tcccacaaga cacgtttcca ttacaaggag catctatgaa tatatctgca tttcttgcaa
     9661 cctctagtaa tatatccccc tcattacaat aaacctcttt attgtttggt gtaaaactta
     9721 cttttatcat gttatccctc ccaatttgtt ctatatatga taaaaggaaa gtatagctaa
     9781 atttgcgtgc actcaaattt tactatactt tccccaatta taataatatc ttaatcttta
     9841 attattctaa tccagcctcg tattctttat cacttaataa cgtatcaagt tcagccacat
     9901 cagctagttt tacttttaat atccagtttt catatgggtc ttcatttata cattctggct
     9961 catcttctaa tttttcatta acttccaata cctcaccatt aacaggagat attaaatcag
    10021 aagctacttt agaagactca actactccaa aatctactcc cttagttaca gtatcttcta
    10081 cttctggtgt ttcaacaaac aatatttctc ctaattgatc ttgagcatag tctgttatac
    10141 ctatatatac aacatctcca tcaattactt ttacccactc atgatcttta gagtatttta
    10201 attctggtaa taatttcatt tcgatagacc cccaatatta tattattgat taaattactt
    10261 aaatcatatt aatattatat catattattt taaattttcc atcaattaca ttacactttc
    10321 catagctaat gctggatgac ctttttcttc taagaaagct aatactcctt cagaatcgtc
    10381 agctatagtt tcatcacaaa ccatatcaca gaagttttct attccagtca tttctttaac
    10441 tgtagcattt aatttatcag ctacataatc ttttaattct tttggcatcc aaactattct
    10501 ttctggtcca ccttctgcat aagcgaattt tttagaagat atgaaatgtc ttccgtgtcc
    10561 cataaatcct ggagtttgaa ctcctccacc tgtcatagaa gcaagttctc cgaaagtcat
    10621 accaactgga gtaacacttg caaattctct gttaactaca acaaatccat ttgcttctgg
    10681 cattatacca cagatacatt cgaagcatcc acaagaagtc attgggtctt ctagtataga
    10741 gtataatgta acagattcaa ctgcaccttg agatatttgg tttacagttt catttactga
    10801 attccataca cctgttctat catctaagca ctcgcctttt tctattggtt gacaaggacc
    10861 tgttgggtct agttctttag tagccttagc atctaaccag ctaactgctc cacaaagacc
    10921 aagtctttca ggagtaacaa cacaaacgtg agctggtgca aatgattgac ataagttaca
    10981 agaatagaaa gtgtcaacac tttcatcaac taatgaagct aatctttcat ctctagcatt
    11041 gtacttagct attgcttcac cttttaattc agaaactttt tcagcatctg taattatagt
    11101 tatttcacac ttatcaacta ctgattcaaa ttcatctaac atcttagcat ataaaacttc
    11161 tcctatatgc tcaagtctaa atcctttatc aaatgcatct ttagagattc ttacccaagc
    11221 catatctctt tgtccaacgt gcattacacc ttctatatag tttaagaagt agtggaaacg
    11281 tctttcaaga actggctcga aatcttcttg catattttta ccagctattt taactattac
    11341 agcaagtggt aatcttaata caccatctgc atcaacttca tctatatttg gtcctattat
    11401 ttctatctta tggtcttcaa cttctgttgc ttctttctta acaacaagct cccaagcttc
    11461 agttctgttt ccaccaaact cagcaaacat atcgctcttt ctaattctct caccttcaaa
    11521 tgctgctgca aatgaaactg ggataggtat ttcagtaact tttattttta ttcctctagc
    11581 ttctaatgaa gtagctacta tcttatcgta atctttttga gttaataggt tcattggaac
    11641 ttcaagaact gtttggtcag ttataactgg gaatcctaaa gctattgctc cagcacctgc
    11701 agatacaact aattcactta atggtccaaa tgcattaaca aatgcaggaa ctctattagc
    11761 tgtgtactct aataatccgt ttaaatcacc aggagttaat ccaccgaata ttaatgctgc
    11821 tcttactgca acagatacaa cgtgtataac agaagttaca tcatatccta gaggtataac
    11881 tcttagctca agacccattt ttactccagc ttctatagct tggtctataa ctttaccaac
    11941 taagaaagtt agtaaacctt tagattggta atcttttata acttttgctg ctgattctgc
    12001 atctggacac tcaccaagaa caactgcaac accaggtata tctcctgtaa ctaatggtac
    12061 accaagtgat ctgattatag ggtcagttat atgacctgca catggctcac tatatggagc
    12121 atccatagtt gaatatttta atgcttcaat tacttctgct gctaaagctg tagctaaacc
    12181 agcattaaaa gcatgttcta gtaaatgagt tctatttatt aaagatttta ctacttctaa
    12241 agcaccttct aagtccccta aagtgttcat cttttgtcct gttgcagcat atatacaagg
    12301 taatgaatat gctgtatcag ggaaagcaac tttatgctct tttccattct tttctatagc
    12361 ttcagctaac atagcttgag ccgcacctaa agcttgttct gaccctgtaa agattatatt
    12421 atatagattc attctatttc cccctttaat atttacacct attttttatt aactataccc
    12481 taaaaaccct agaaagcaaa tccaccttca tttaactcta agaaagagtc tgcatataaa
    12541 gatgccccgt tgattatgtc acaagatttt acagttttca ttaattctgg gtcacatgga
    12601 ttcattatag ctgaactaaa tccattagcc attgccattg ctaagaatgc actatctaaa
    12661 acaggtctaa catgcttagg acatccatta gatacgttag ataatcctcc tgtagttaaa
    12721 agtcccatct cagtcatcat ttttatagct tctaaaactt ctacttgttt ttcttgcata
    12781 ccttttataa ctaagcataa tgggtcaaat agtatatcag ttggctccat tcctaacatt
    12841 aagccttttt ctaatatttc ttggcagtaa gccatacgct catcattatc tcttgggata
    12901 ccttcttttg cacataaacc tataaccatt gcttcgtatt cagctgctat atctattaaa
    12961 tcaaatcttg aaccagcatc agcagagttt attattggtt ttgcattagt tctatcataa
    13021 acttttagtc cagcttcgat agctttttta ttagctgtat ctaatgctat aggaacattg
    13081 ttgaactcag attgaagtaa tttgattccc catgtcatta tttcttcgcc atctctttcg
    13141 gcaggtccta tattaaaatc tatatagtgt gctcctgctt ctaattgctc ttttgctctt
    13201 tttaagattg gagctgggtc tctttctgct aatgcttttc ttattgaagg tgatatacaa
    13261 tgtattctct cacctataat cataaatttt tccatttata atgtccccct ttaaatttaa
    13321 tattaatagt tagttaatat tttattagtt tgtttggcat gctttttctt ggtatccttt
    13381 taagaactta ggtaattcca tagattcctc tggtcctata actacattcc atcctggtaa
    13441 gttatcttct atatcacctt taagtactgc aacttttcct ggaatgataa ggtctttaca
    13501 attagttact tcttctacct tactttcttt aatgaaagca gagattgagt ttccaccaaa
    13561 tttaccagca gcccaagatg taagaactga atatccacct gcatctggta ttactaacca
    13621 tactggaact tttgatcttt ctatatcacc agcaactatg aagtaagtta atgcaaagtc
    13681 aacagtaact aatactggag aattttcatc tgggttgttg attggataaa tctttggttc
    13741 aactctcatt ggtctttgtg ggtcagtgta tatgttttgt cttagtgcaa ataatggtaa
    13801 tgccttagca taactaatat catctattac tattatagaa ccgtatttta tagtgaatat
    13861 tgatgataaa gcaacttcca tcatagggtt tgaattagat aatctatttg caaatactat
    13921 tgaaggatat ccaaatgttc tatcttgctc ttttaaagct gttcttctaa cttgtatagc
    13981 attagtgtaa gtatctttta tgttttctcc tgttacatct aatactaatt ctttatatcc
    14041 agcggcttga attaattcaa ctgtttcata taattcttct aagctaccag cttttacacc
    14101 taatggtaaa cttgctcctt taactacttc tatcatatct ttgtagttct ctttagttgc
    14161 tccatatacc attggtctag catcttttaa tacttcaaca gcttctttaa ctacttgtgc
    14221 atcatcacaa gctaaaatat aagctacttc taatccactg ccttttattt tatctattaa
    14281 ttttaaatac gcatctttat ctccacaata ctctaaaaca gccatttcaa ctttcatttg
    14341 ttcaccaatt cttacatagt caactttttt catgttagct attttagcat ctactgcttc
    14401 atcactcata caagtacaga atgaaactgc atatctattt ctacttacta atgttttttc
    14461 atgtctaaat aatactgttt ctccacctag ttcatactca gatgcaccag caccaacttt
    14521 taaagctttc attaaaggtg cagtagcctc tgataatttt gctatagcgt catcagacat
    14581 atgtgggcat ttacctactt ctacagctcc tgaagcaacc ttcatagaga aagccataca
    14641 agttgggaat ccacaatcct tacagttttt ctttggtgtt aatttaaata tatctaaagc
    14701 ttttaatgcc attatttttt ccccctaacc ttaacaattt aattaagcta atgcgttaac
    14761 caattctttt atagtttcta ttgattttgg atgacgaagt ataactgcgt ttgaaccacc
    14821 tactaaaaca cttgctgcag ttgaaacttc cattgctata gttctttctt ctggacaacc
    14881 ccaatctggc tcatcttcta ttggagctat agcttcttta acatgaccta cttcaaaagc
    14941 tactggtgtt atgataggca tttgtaatgt cttatcattt tgaccaaatg ctgcaagtct
    15001 aattctatcc atagtagatg caacgtactc atatccataa ccaactgctg aacatccaac
    15061 attcataact atattttcac ctttaacgcc tagttgagtt aacaatacgt ttaattgttt
    15121 agctaggttt atatcaacag aagattcagc tcctacttta tgtgcataag ccataccagc
    15181 tgatgctcct actcctttgt agttatcttc tactgctgac ataaataagc agttatgtcc
    15241 atccaatgtt tgagctaatt tttcaaataa tttaccatct ttttcatgat ttcctgaacc
    15301 agctacaact aaaggtaatt ttattgcttc aattactgcc ttagctacat cagcacattc
    15361 atcaactgac ttatctaatc catttgggtc agaaccatca aattttaaac aaataaaatc
    15421 tgcttgtgta ttagcttcaa catatttagc ccactctact ggacaattag ctacatcttt
    15481 gtataattcc ttatatgaat cagtccagct ttcaggataa acgtctgata tttgtatccc
    15541 tatttttgga gaattaccta cttctccatc aaaactataa aaaggtaata cattttcccc
    15601 acctaattta attgcttttt cccctattcc tacttcaact tctgatattt ttccagaata
    15661 tttttgagta gacattttaa atgccatatt tttttccccc ttaagcctat tttaattcta
    15721 atttagcaaa aatttctttc atagccactt tagattttga atcttcaggt aaattaacta
    15781 aaggaatacc tgtagaatca tattcatata tcaattcatc cataggtaca acaccgatta
    15841 gctctaggtt atgcttttca atttcttctt taacaccatc attcataact ccatttggaa
    15901 ctttatttac aatcaaaagt attcttccaa cacttaactt tagttcctca gctaaatctc
    15961 ttattctagc aacagcttgt atgctacgtc ttgagcaatc actaaccaat aaaagtgtat
    16021 caacatgtct agtgactttt ctacttagat gttccatacc agcttcgttg tccatgacta
    16081 agtatttgta atgaccagat attttgttta cttgttctct aagtatccca tttacaaaac
    16141 agtaacatcc ttcaccttct gacctaccca ttactaataa gtcatatcct tcgccttctt
    16201 cgattataga atttaatcta aattgaagat attgtgcctt tgtcatacca ccagggaatg
    16261 cattgcctaa cttttctctt tgatttactt cttctcttat ttctcctatt gttgcttcaa
    16321 cttcgatacc taatacttca tttatgttag cattggcatc agcatctaca actaatactg
    16381 gtcccttttt gtccttaacc aaataatcaa tcaaaagccc tgtgagactt gttttaccag
    16441 ttccaccttt tcctgcaaca gctatattgt atcccatttt aagacttccc ccttaaatct
    16501 agccacagag ccttatgtac tctgtctgtc tattataatt taggtacaga atgaacacat
    16561 tctccatgta catcatgaag tgcttccatt agcccttcag ataaacttgg atgtggatgt
    16621 attgcatccc ctacttgttc tactgttaat cctagatgaa cagcaagtga caactctgtc
    16681 aataaatctg tagcatgagg tcctactact gctgcaccta ttatcttatc agtttctttg
    16741 tcagctataa cttttacaaa tccttggaaa tgtcctatag cttgtgcttt tccaagacct
    16801 ctaaaatcaa attgacctac attgtattct acaccttcag cttcaagttg cttttcagtc
    16861 ttaccaacac cagcaacttc aggttcagta taaacacatc ttggaattgc tctataatct
    16921 actaccttag tcttacctaa agcattttca actgctacga taccttcttt agaagcaaca
    16981 tgtgctagga aaggagtatc tattatatcg cctattgcgt atataccttc tacattagtt
    17041 tcaagatgtt cattaactac tacttttcct ctttccattt caattcctat atcttctact
    17101 ccagaattgt caaggttagg tcttctacca acacatacta atgcatattg tgcttctata
    17161 acctttccat tagaaagagt tgcaacagcc ttaccatcaa ctacttcaca agtttgtact
    17221 ccaatacctg taataacctt aattttatct ttcttaaatt gtctaagtag ttgtttagct
    17281 acatctttgt cttcatttaa tagtatttgg tcaaccatct caactatagt tacttctgtt
    17341 cctaaggctc taaagaactg ccctatctca catcctataa ctccaccacc tactattaac
    17401 atagactcag gtatctcttc aagtccaaga acttcatcac tagttattac aacttttcca
    17461 tcatatggga acattcttgg tacaactgga acagaaccat ttgctagtat tatcttatct
    17521 gcttttatag tttctactgt tccatcatct ttagttactt ctatagtgtt tttatcaatt
    17581 aattttccaa aaccattaac taagtttact cctctttttt caaataagaa ctctatacca
    17641 cttattaatt ggtttacaac tttatttttt ctttccatta tggcagtgaa gtttggctta
    17701 actgtaccat ctatttctat accaaaatcc tttgcttctt ttacagtatt caatactcct
    17761 gaagaggcaa gaagtgcttt agttggtata catcctgcat ttaagcaagt tcctccaact
    17821 cttctttttt caacaactgt tacatctgca ccaagcatag aagcttttat agctgctaca
    17881 tatcctcctg gtccaccacc tactactact atcttcatct ttaattcccc caaacctaat
    17941 ataatctaca atcttgcaat actataaacc tgcggcatcc agtatagcag gtatattctc
    18001 ttctgcccca attgccatta tatgaacacc atcacataaa tcttcttctt ttaattgttt
    18061 gataagttcc cctgccattt ttattccttc acttacccag ttttcttttc cagctgctct
    18121 taatctttcg atttgttcat ctggaacaaa tattcctggt acatttgcag tcataaattt
    18181 agccattcct ggtgatttta aaggtactat tcctgctaat accttgcaat ccatatccct
    18241 tgttaattct ctaaactttc tcattgtatt tatgtcataa agtgcttgtg tttggaaaaa
    18301 tttagcccct gcttttattt tctttttcat ttttaataat tgaacttcta ttggagaata
    18361 ttctggagtt actgatgctc ctagataaaa atctggagaa ccttttaact tattaccagc
    18421 catgtccgta cctgccatta aagtctcagc agtttgaagt atccccacag aatctaaatc
    18481 aaacactccc ttagcttgtg gatggtctcc tacgctagta tggtctcctg ttagtgccaa
    18541 cagattgttt atcccaaata cacctgctga caacatttct ccttgtatag ctattctatt
    18601 tctatctctc cctgttattt gaataactgg ctctaatcca gcatctttta ataatttaca
    18661 tgtagcaaga gaagttgctc tcataactgc cgattgaaag tctgttacat ttgctgcatg
    18721 aactcttcca actaacggct ttgcacattc tattaaatgt gaaaggtcag tcccttttgg
    18781 aggagccatt tcagtagtaa ctgcaaattt tccactttct aatgtttctc ttaataagct
    18841 catctctaat ccccctttta accttctaaa aaaaactttt atgaatttgc tgctgcttct
    18901 ttctttttct ttgcacttaa acttcttgga ttattttgct tagaatagtc ttttggtggt
    18961 cttatttctg ctaagttatc taattgtcct atagcttcta gtctttcata tatctttatc
    19021 caagcacaat cattctctga gtttacttca cacttaccat ttttagctcc accacaagcc
    19081 ccattcataa gacctttagc acacatagtt actggacata tcccacctgt ccaaccaagt
    19141 tcacagtctc cacaagcctt acaggcttct tcatattctc caactctttg tacttcccct
    19201 ataaacaaag tattgttggc tggataaact ggcttatctt ttaaattctt aactattgtc
    19261 tgtgtaccat ctccacatgc taaagataaa actgcatcag cttccttagt ctcttctttt
    19321 agagccttta aatccttttt agactttaaa aggttacaag cagggtcaag cattacatat
    19381 cctaagattt ttttgccttc accttcaaga gtttccttca tttttaaaac ttcttcttcc
    19441 ccaccacttt tacaagttgc tgcacattga ttacaaccaa ctaaaaccag cttgtcaaaa
    19501 tcttttaagt accctagcac ttcttctaat ggtttatttt cagaaattat catatctact
    19561 tcccccctaa accttccaca tatatatatc tatttattgt tttgtaattt acaagcctta
    19621 actacatgtt ttgcaagtat agatgttgta actgaaccaa cccccgctgg aactggagtt
    19681 atcattgata ctttgtctaa aactgcattt gtatcaacat ctccacataa atttccttct
    19741 tcatcaacat ttataccaac gtctataact accgctccat ctttaacaaa actttcatca
    19801 accatcttag ctctaccaat agctgctatt agcacgtctg cctctgctgc tacacctgat
    19861 agatttttag tttttgaatg acatattgta acagttgcgt gttcacttaa taaaagcatt
    19921 gatactggct ttccaactac cattgatctt ccgagaactg ttaccttact tccttttaaa
    19981 tcaacattat agtgttttaa tatctcaact actgctgtag gtgtacatgg tggaaatcca
    20041 cttttatccc cttccattac cttagcagag tttattggac taaaacaatc tacgtctttt
    20101 tccggtgcaa taacatattt tataacttct tcatttaagt gttttggaag aggtctaaaa
    20161 cataatatac cattaacatt tttgtcatca ttaactctct ttaatacatc aatatattcc
    20221 tcttgtgtta tatcctcagc taactctaac acttctgttg ttatacctat gttttgacat
    20281 ctctttagtg ctcctctttc ataagataaa tcatcacttc ttgccccaac tctaactatt
    20341 gttagtttag gatttatacc ttctttaact agtaaatcga cttcttttat taattcttca
    20401 ctaattttat cagctacagg ttttcctttt ataatttgtc ctttagttga cattccttcc
    20461 atacattcac aactccccta caagcaaact taaattaatt tattctatat accacatatt
    20521 aataacatag tatctacttt cgtcatttaa aaattatttt ccaagagctt tttcaacttt
    20581 ggcataaact tcatcacaaa tttttacacc atcttcaact aacttatcta cttctgtttt
    20641 taccttatta acatattctt tatcttgtat agaatttata tttataacta catttaattg
    20701 tccaccaagt atagcagctc ttaaacactg aactcctaca ccaacatcac ttatagcaag
    20761 tcttgaacct ttatcaacta aatcttcatg taacttaata gcttcatagc aaactcttac
    20821 tatgtttaat ggtacttcac aagcaacttt taatgccttt tccattgttt ctgcttttat
    20881 tcttttttct tcttctgttt ctttaggaag cccgtaagct tttgataatg gtaagaaaca
    20941 ttctgcatca tcatctatca tttttaataa atctttttct aactttcctg ccttacttaa
    21001 tatctctttt acactttctt catactcagc atacttcttc ttccctattg taaggttaca
    21061 taccatacta cctaaagcca tacctattgc acctactagt gcagcagctc ccccaccacc
    21121 tggtactgct tctttggaag atagtacttc tacaaaatca acacaagttt tatctgctat
    21181 tttcatgata atcctccttg aaaggatgta agaaaatatc tcaaattgtt cgagatattt
    21241 tacgaactat tttaagatat ctcctactct ttatttataa attaatttcc ttagaataat
    21301 ccagctatta ctccattttc atcaacatct attttttctg ctgctggaac ttttggtaat
    21361 cctggcatct tcattatttc tccagtaagt gcaactataa atccagctcc tgctgaaatt
    21421 gttgcttgtc ttacagttat tctaaatcct gttggtctac ctaatttagt ttggtcatca
    21481 gttaaagaat attgagtctt agccatacat actggaactt tagtaaatcc aagtttttct
    21541 aagttagcta tttccttatc agcttcagga gtataatcta caccatctgc tccataaatc
    21601 ttagtagcta tagcatttat cttgtctttt atagataaat catcttcata gcagaatcta
    21661 aagttatttt cttcttcatc caataatctt aaaacttctt tagctactgc aattccacct
    21721 tctccacctt ttgcccatac ttcagaaaga gcaacattaa cacctaattc cttacacttg
    21781 tcttcaacaa gtttaagttc agcttcagta tcaagtggga atctgttgat tgcaacaact
    21841 gctggtaatc catatacttg agttatgttt tcaacatgtt ttaataagtt tggaagtcct
    21901 ttttcaagag cttctaggtt ttcgttattt aagtctgctt tagcaactcc accattgtat
    21961 tttaatgctc ttacagtagc aactattata acagcatctg gttttaagtt tgccattcta
    22021 cattttatat ctaagaattt ttctgctcca aggtcagcac cgaaaccagc ctcagttatt
    22081 acataatctg caaagtgcat agccattctt gtagctataa cagagttaca tccatgagct
    22141 atatttgcga atggtccacc gtgtacaaat gatggtgttc cttcaagagt ttgaactagg
    22201 tttggtttta aagcatcttt taataatgca gccatagcac cattagcttt taattgtcta
    22261 gctgttacag gttcaccttc gtagctgtat ccaactatta tgtttcctaa tctttctttt
    22321 aaatcagata tatcacttgc caaacagaac gctgccatta tttcagaagc aacagttata
    22381 tcgaatccat cttgtctaac tgctccatca ccttttttac ccataccatc aacgatattt
    22441 ctaagttgtc tatcgttcat gtctacacat cttctccaag ttattttctt agggtctatt
    22501 ctaagtgcat ttccttggtg aatatggtta tcaagcatag cagctaaaag attgttagca
    22561 gctcctatag cgtgaaaatc tcctgtgaag tgtaagttta tatcttccat aggaacaact
    22621 tgtgcatatc ccccaccagc tgctccacct ttaacaccaa atactggtcc tagagatggt
    22681 tctcttaaag caactaatac atttttgtct agtttagcaa atgcatcagc aacacctata
    22741 gtagtagtag tttttccttc acctgctgga gttgggttta tagctgtagt tagtattaac
    22801 ttagcttttt taccagtctc tctttttagt agattgtagt caacttttgc tttgtattta
    22861 ccataaagtt ctacatcatc ttcacctagt cctaattttt ttgcaacctc tctaatatct
    22921 tgaggcttag cttcttgagc tatttcaatg tcagatttaa atcccataac tgaatctcct
    22981 cccacaacct aaatagattt aaaatataat ttttgtaaaa attgtattaa tatgtaaaaa
    23041 ttatatttta taagataatt atacatgtaa aattacttaa aaactagcta ctaagctatt
    23101 aaaatattca aacttaatag ctagttattt atctaattac ttacttatta cctatctatt
    23161 gcctatgtct aacctttaac caacttaaaa tcccaaatca aaatttctat ctattttatt
    23221 tatccttaag tgacattaat ttatctttta ttgctttaat ctgttctttc gttgtttcac
    23281 tagaatcata tggagattga ttggctctat cagaatcaat aacatcttgg ttataataaa
    23341 taaatcctaa agattctcca tcttctaagt tttggattat aaattcttca tcttccttat
    23401 ttctaatttt atttcctact acaaatactt tatttacacc tatatcatgt ccaagtttct
    23461 taacttttct ataagtctgt agacttctct ctcctggttc tacaactact ataaaagcat
    23521 ctacaccttg ggctgttcct cttcctaagt gttcaattcc tgcttccata tccataacga
    23581 ctacatcttt attttgtagt attaaatgtg agcagagtct cttcaaaaga acatgctctg
    23641 gacatacaca tccagtacca ccagaatcta cggttcccaa tgtaagtaat cttactccat
    23701 tgtattcttt acagaaattt tcagggatat cgtctacttt aggattcatc ttaaacattt
    23761 ttccaaatga acctacagaa gcagctgttc tatctgaaac taattttttc atttctgata
    23821 taggtactat tgattcatat acttcttttg gaaaacctag tgctaaagct aaattagcat
    23881 cagggtcggc atcaacagca acaactctgt atccgtcttc agcaaacatc ctagagagca
    23941 ttgaagaaaa agtcgtttta cctactccac ctttacctgt tattgctatc ttcataatcc
    24001 ctaccacctt ttattactat ttgcttttta gtagttaatt ttacagttaa atatttttta
    24061 tactctatat ttttatacac taaaaataaa ctacagctag tccttacagg aaccaattaa
    24121 atttacccta ctagcttttc aaagcagcat gtatagtttt attattcttt aatattgttt
    24181 atattttatt tattcagtat attcattata ttttgatgtt ctttacttta atatttgact
    24241 ttattcaact ttactcaacc agaaacttct tataaaaacg tttattctat aacttcacta
    24301 ttttttaagc ttcagccttt tctgccattt tttcttcaac taatgcttca aagtgaacac
    24361 gttttttctc tatatcttct attattgtag ctgctaactc ttttggattt gtatttacag
    24421 taaatgtagc tccaacttta tcttttaatt cactagttaa tatatccact gctcttgaag
    24481 aaccagctat tggaggcatt attcctaaat aagtgtctat accagaagct acaacatagc
    24541 atcctatagc aactgctttt tctgacatcc actctggagc tatacctaca actggaaggt
    24601 cacacatatc catatctaag taattagctg ctgctccaac caaatctagt atacgactta
    24661 tatcaacaca agaacccata tgtagtactg gtgggattcc tactaattta cagacagttg
    24721 caagaccttt acctgcatat ttttcagccg catcagattc cattaaacca aacttagctg
    24781 ctgctgaagc tccacaacct gtagtaacta ctattatgtc gtttttgatt aattctttca
    24841 ttattgttac gtgagcctca tttgaaactc ctttagcatt gttacatcca actacaccag
    24901 ctgctcctct taatactcca gcttttagac agtctgttaa tggcttaact gttcctgctg
    24961 ggtctatatg agagtttaca actctgtcta attgatttac tactgcttct acactatacc
    25021 caactgttgc gcctgatttt aattcaggta taagtacttt tgatttatct ctatttttaa
    25081 agtttaaaat agcttcttta actattgctt tagcatcatc aagtgcttgt tcttctctaa
    25141 attcaatata agttgaacct gttatcttag cttttggaga agttgttaca aatttagtat
    25201 ggtaacaatc tgcaactcta gctaatgcag ggaatataca ttgaacgtca actattacag
    25261 cttcaactgc tccagttatt attgcaagtt cttgttgatg gaagtctcct gctattttta
    25321 caccatgtct cattgtaatc tcattacctg tacaacacat acctgctaag tttattccat
    25381 cagcaccaac ttctttagct aaagctacta agtctggctc ttctgatgct aaaactatca
    25441 tttctgataa tgctggctca tgtccatgta atattatgtt aactttattt tcttctaata
    25501 ctgctaaatt cgcctctgtt cttcttggca ctggagttcc aaataagata tcgcttagac
    25561 gagtacctat catagaacca ccccaaccat cagccataga agttctaaga ctcatgtgta
    25621 ttaaactatc tatatcacca gtacatccta tatgagtaga gtgcattatt gttgcaattt
    25681 ctctatctat agctcttggc tctatagcgt attctttcca tattttttgt cttggttctg
    25741 gtgcattttt tataaatctt aatgtcccaa aaggttttcc aaattccatt aatgcaattt
    25801 ctgccacttc atgtgctaca tcatagatat ctctaccttc tgtctctaca tcccattctt
    25861 tagctaaagt aattaactta gcttcgtctc ttattttata gtttccattt ggatcagcta
    25921 atgctaatgt atgtgctata tctctagcat ggtctgaatg ggcagctgtc ccaccagcaa
    25981 ccattcttgc aaagtttcta cctactatag tatgctcatc tgctccacat aacccccttg
    26041 gagtcttagg gcttattcta catggtccca ttccacatat tctacagcaa acaccttgaa
    26101 gaccaaaacc acattgtgcc ttaaagcctt cttttctatc atacattgtt tctacgcctt
    26161 cttctctagc tttagctatc atcttctggc tgtttaaatc aattgttaac attttttcat
    26221 ccatattcct atcccctttt tttgattttt aaaatccccc aggtgactga cttcatgtca
    26281 taaaataata aaataaatgc tttctaagtt gattatatac taattttcag tgctttgctt
    26341 tagatttaaa tattttttta acaaaattag ataatacata aaaattttat tttctacact
    26401 taatttaagt tgattttatc gcttgttttt ggctatttgt taatatctaa acctactttt
    26461 ttgatacttc agtttaatta aattatgttt tgattttttt aaatcaaatt tgaaattttt
    26521 ctagtcattt ttatattatt gcagattgtt tgtgtattta ttatctttca ctactgtttg
    26581 gacttattac agatttttac tccctaaact taattagtaa aaatttctta gaatagcatt
    26641 ttttggacta atttttttga atttttttat ttttcctact ttaatattaa ttatagcttc
    26701 aaattgaaca tttgtatata ttcatttgaa gctataatta ttttttatat aaaacttttt
    26761 taatacagca ttaatttaga cattttgact attttttcta ctttaattca tttatttcaa
    26821 gcgatttatc ctatttcata tttttagcta caatagcccc atatgcaata gaatataatt
    26881 tagattcatg tgaatcagct cttgacacta aaactatagg agcttttgca cccataatta
    26941 tacctgctga tttagagtct gccatatatg taaaggcttt ccctattcca ttaccaacct
    27001 ctattgttgg aaccaatatg atatctacat ctccagatac ttcactttta aaccctttta
    27061 tctcactagc ttcttttgaa acagctaaat caaaagccag aggaccttct acaattacat
    27121 tttcaccaaa ttctccttct ttacaagctt ttgctaacat atctgcatct acagttgctt
    27181 gcatcttagg atttactttc tctttagctg ccaaacatgc aacttttaca gtttccattc
    27241 ctaaagcttt tgcagctttt atagaatttt ttaatatctt tgccttttgt tcataatcag
    27301 gagctatatt catacctcca tctgtagtta taagtaattt atggtatttt tctaactcat
    27361 ataccataac atgacttaat aaactatcag ttctaagacc atagtcttta tttaacactt
    27421 cttttaatat gacagatgta tctaataatc ctttcattac aaagtcggct tctttacttg
    27481 atactaattt aactgctatt tctgcacact ctttgatatc atccttatcc actattttga
    27541 taccacttaa atcaaatcct atttcttttg atatttctct tattttgcct tcatttccaa
    27601 ccaatatagg agtaattata tttctttcta cagcatcctt tattgccatt aaaacttctt
    27661 tatcatgagc tgctgctaca gagagtacaa ttttctctcc tcccttaagt tgttctaaaa
    27721 tatcatctaa tttttttatc aaaagtaacc cctcccttat atatatttaa ctaaataata
    27781 ccatatttgc tatacctttt ctcaatttat tttttatgtt ataaagaatt tatataacta
    27841 aaagacagtt taatatttta ttctgggatt tcatcaataa ctcttaaaag ctttgcagga
    27901 acaccaccta caatagtatt ttctgcaaca ttttttgtta caactgctcc agcagcgata
    27961 attgcattat ctccaatagt aactcctgga actacagtta cattagcacc aatccataca
    28021 ttttttccta ttgtgattgg agaaggataa gttgtaccac gtttctctgg tgcaatacca
    28081 tggttaagtg tagccaatac cacattatgc ccaatttgcg tattatctcc aatctcaatc
    28141 cccccctggt cttgaaaatg gcaactgcta tttataaaaa cattttttcc tattgtgatg
    28201 ttttttccac aatctgtata aaatggtgga aacataaaaa atgattcatc tacctttttt
    28261 ccaattaact cagaaaataa actacggact tcttctggtt catgataaga agaatttaac
    28321 ttacttgtga ttttcattgc ctcattactc aacttaacca taaattcatg tatttctgat
    28381 ttactagaaa caggttttct ttgattgata tgtgttaaaa attcattgat attcatttta
    28441 tacatccttt caatatgtgc tttaatttat ttgtacattc taaataatat tttaataaaa
    28501 attaatttaa tttattttta ttaaaatatc attgcttctt ttagtgtaat atacaattat
    28561 atctatatca aatatctata ttaaatactt aactatactt aaaaagcatt taaattgata
    28621 taattatata ataaataagt taatattgag gagggataat tatggaaata agagtattaa
    28681 gatatttttt agcaattgca agagaagaaa gtatatctgg tgcggcaacc tttttacata
    28741 taacacaacc aacactttct agacaaatta tggaacttga agaagagttg ggagttaaat
    28801 tattggttcg aggaaataga aaaatcacct taacaaatga aggtaggctt ttacataaaa
    28861 gagcagaaga aattatatca ttagttgata aggttgaatt agaatttgac aatcccaatg
    28921 agattattag tggtgatata tatataggaa gtggtgaaac tgaagctatg cgacttatag
    28981 cgagaactgc ccatcatttt catcaaaaat accccaatgt aagatatcac ctttttagtg
    29041 ggaatgctga tgatgtgact gaaaaattag acaagggact gcttgatttt ggtattttaa
    29101 ttgaacccgc aaatatacag aagtatgatt atgcacaaat tcctgctact gatgtatggg
    29161 gtttactaat gcgtaaagat agtgcccttg catcacaaag cacaataaat ccaaaagatt
    29221 tatgcaatat acctcttatt atttcaagac agacacttgt tgataaagca atttctaaat
    29281 ggcttcaagg cagtaatgaa aaactaaaca ttattgcaac atacaattta gtttataatg
    29341 cttctctttt ggtagaagaa ggtctgggct acgctctgtg tttagataga ctcgtcaata
    29401 ctacagaaaa cagtaatctt tgttttagac ctctatctcc tcctttagaa gctaaattaa
    29461 atattatatg gaaaaaacat caaatatttt caaaggcagc agaaaaattt ttacttcaaa
    29521 tacaaaaaga tttttcttct atcatgttgg atacatctaa gttggataca cccaaagaaa
    29581 aataaatcat aataaaaagt gagagtgtct caaaatgaac tttgcactgt ataaggcact
    29641 cttttttata caaagtaaaa tatatgttca tcatttcagc actaaaaaag aggcttatct
    29701 ctattttgag atagccccac ttaaccaata aaattaaata caacttaaca atatccttac
    29761 ttatttattc caagttctct tagacaagca atagtaattt catcttgtgg atttaattca
    29821 taagctcttt ctatatagta agttgcatca tcaatatcac cattatttaa ataagccatt
    29881 cctaaattgc aaagtatttc tgggtcttcc tttatcttag ccgcttgttc aaaatactct
    29941 attgctttat ctaaattatt cttataagcc tcacaaagcc ccaattccac tatagtatcc
    30001 acttgattag gctttattat caatatcttc tcaagataca tcttagcctg ctctatttcg
    30061 tccataccct tatatccaag tgctatcata aacagcaagt tccaccaatc catatgttct
    30121 tcttctaatg gaagtaactt ctctaatcct tccttaaact taccttggaa tacaagtgta
    30181 tatccttctt catattgaac tttaaagtcc atcttgccca gattctcttg tacctcagct
    30241 actaaatcag catctaaccc tagccttaac gcttcttccc aagtcagctt agtctttaaa
    30301 tattgacctt gattataata atggtagcct aagtgataat aacctaatgc aaaattctca
    30361 tcaacatcaa ttaccttttc aagcttagcc atggcttcaa gtaagaatgc attcattgcc
    30421 ttatcatcca tatctttttg atactgttta gctatctctt ggcaaacaat cgcatagtta
    30481 tacattgcat ctaaatcatc tggatacatt gttataaatg cttttatata tattaaagaa
    30541 tctgtatgtt caccaatctc aaagtatttt ctcgacatat atcccatata cgatttcaaa
    30601 tccaaatcaa tatcttttga taaagcattt aaaaacttct catactctgc attgttttta
    30661 aacttactat ctattccaat tatatatatc attgcatctg ctatagacat tgaatttaac
    30721 ttatcttgag cagtcttttc ttttatccct tttactaata cctcattttt tataggtaca
    30781 tctaatccac cttttggcac ttcatagctt tttaatttaa actctccacc atgttttata
    30841 gttataaaag ctaattcttc tgcttttttt agcaaatatt tttctatctt aaaatccatt
    30901 ttatcctcct aaatacttaa atctctactt tatgagtttt agatttctca aatatttttt
    30961 tatcatcata ctcaaataag actacatttt ctacttctct tatttctgta tttatatcat
    31021 tttttaaatc cacaacatta aatctaaatg gctcaaaatg aacatatttt attatatgtt
    31081 tagctggttt atcaacaaaa ctaggtaaat atgttgataa gttatcatta tcttgtaaaa
    31141 atacatggca tctattctta aaatcatcca catctagctt attaaaaaac tgtggtatat
    31201 ttgatgtttt acccagtgat atatagtcat attttaatat atcgttgaat acatcattat
    31261 ctatgttgat tttttcatta tagaaatcca tcaatatttt atataactga ttctttcctt
    31321 gtgccaaatc aaagtagcca ttttcatcaa aatacttggc aaaaacctca aagaatttaa
    31381 aagggctttg tttataaaat ctgcctataa tatatctcat agataacaca aagtttttag
    31441 aattgtagta tctctccagt atatcttcta tatcctttag cttcaaagtt tcttcatagc
    31501 ttatataatt attgtataaa aactcatatg gagcataatc cttatattta aaaccatgct
    31561 catctgcaac ttttctcata cctgtccctt taatcatctt taaaaagcct aattgcagat
    31621 gctctatacc tagattaaat acatcattaa aagaattttc aaagctctta taatcttcat
    31681 aaggcagtcc tgcaattaag tctaagtgct gatgtatatt tctataagat gctacagttt
    31741 gtacaacatg agacaacttt ttaaaatcat cccttctgcc tactgcatca agtgtttttt
    31801 cattcgttga ctgaacccct atttcaaatt gaaaaagacc ttctttacaa tcagccaaaa
    31861 actcaagcat cttatcatct atcaaatacg ctgtaacttc aaaatgataa gttgtatatc
    31921 cattatcatt tttcataagg aaatccatga tttccatagc cacttttcta tttgcattga
    31981 atgtcctatc tataaacttt atctgtgata ctcttgcatc tattaaagct tttaaatcac
    32041 tctttatcct gtctaaacta aaatacctta gacctggaat agtagatgat aaacagtact
    32101 ggcagttaaa agggcaacct cttgatgatt cataatacac tattctattt tcatattctt
    32161 ttggattcaa gttttcataa ggactaggaa tctcatccaa atcttgaagt aattccatag
    32221 gcttattcac tattatctca ttgccttgtc tatatacaag tccatttaca tcatttattt
    32281 ccatctttcc ttgcaagtga agtactaagt ctctaaaaat taattctccc tctccataaa
    32341 gtatataatc cacaaaatca tactttttca ttgcctcata actatcataa ctcacttccg
    32401 gtccgcccaa agctatttta gtattaggtc taattttttt tagattatca caaagtttaa
    32461 ctatatctcc cacattccag atatatgttg agaataatat tatatcatat tcatttttat
    32521 atatatcctt taaaatatag tccaaatcgt tgtttatcgt gtattctttc atattaactt
    32581 ctatcaaatc tcttacaaat tcttttagat atcttatagc caaatttgta tgaataaact
    32641 tggaatttaa tgttgttaat aaaattttca aatatttctc acctcttttt aagattatac
    32701 tgtctttttg taccccttat aggttaaaat agtatatgta aagtctaaat aatatctata
    32761 taattaattt attaatttca agtaatgtga ggaaaggaag ttttttatgg aattaattga
    32821 aaaagtatct atcaactctt tatcaaaaag agatttacta cttattataa agtctcttga
    32881 atacactaat gaaaatactc aaattagtga tttcattgac cttaggaata atatagtaaa
    32941 ggaattatgt tttttaactg acactaagga aaaagatttt ttagattatc tagaaaaaaa
    33001 tagcacactc taataaccta aaccttttta aactttaata tataaatacc tgatttagaa
    33061 tttattttta tgcttaatat attatcatta aaggttttcg acataactgt tatttgatta
    33121 aatatagggt ctactatagt attaccttct tcaagtctta taagacttct tgggtctatg
    33181 cttttattat atgccttaac tccaatttta tattttctca atttaacaac atattcggac
    33241 atagaaaaaa taaaatctat attatcttca tttaatttac tatcttcacc caaaatggat
    33301 aatatagatt tttttatgct attaattcct tcattttcta cagttctatc ttccatatat
    33361 acatcatata gaacatttaa aacatttaat aaaatacttt cttcatcata atcttcagat
    33421 ttcaaatcaa tgtacttatc aattaattgt attgctttaa tccttgccat ctgaaatttt
    33481 acaacatctg atttgtctaa ttctatagaa aatcctatta tcttgtcttt aatactctgt
    33541 agtaattcga catactctat tttactatta tttataagat tgtgcataat ggaattatac
    33601 actacagaac ctaataaata atcaaataaa agttcttctt ttttaaaata aataacatac
    33661 ttttttatta gtttaataac ctcatcttca cttttttgaa tattagtatt tgataaaatt
    33721 agtggtaata aaatatttaa aaaattgata tttcctattt tttctccata aaatctatag
    33781 taaataagtt catctctcag tctattttcg tctatataca cacatctttg cgttttatca
    33841 tctattaagg attttgtatt tattatagtt aaatctttaa gagccctact tgcactacta
    33901 aaagaagatg tgtagacttt ttttagtaat tcgtcattca taaatatatc ctttctgaaa
    33961 aattgaatgt aaataaattg ttattaaaaa aaagagacag cccgtttaag aagctgtcat
    34021 atataggggg atagtagtgt acaaaaatca ttgtaatcta ctatctatat attattatac
    34081 gatactactt ttttatatgt aaagcatcag ttttcgacat ccctatattt tacagttata
    34141 caaattgcgt actaagttca attttattct ttttatttca acattttatc atgctacatt
    34201 ctttcaggag cgtgcattcc caacaatttc agagaatttt ctaaagtttg ttttgttgct
    34261 aaaactaatg caagtcttgc ttttcttatt tccaaatttt ctactaaaat tggattgtca
    34321 tgataaaatt tattaaacgc ttgagctaaa tctagcataa atctagtcac tatatgtggc
    34381 tcatttttct ttagagcaag taaaatattc ttattgaatg attctataac tctaagaact
    34441 tctgcactat cttcatcagc taatagacta taatctatat cagaagtcac ttctacttct
    34501 gcttttctta aaactgcaca gcatctagca tgtgtatact gaacatatgg tccagtctca
    34561 ccatcaaaac taagcgttct ttcccaagag aaggtatagt ctttaattct actattagat
    34621 aactcttgga atactactgc cccaacacct acttgcttag atatttcatc cacattttta
    34681 gcatttgggt ttttagctag tataatttcc ttagttttat ctatagcttg atttaaagca
    34741 tcttctaaaa atactactct tccttttcta gtcgacatag taccttcttc aagtgcaacc
    34801 ataccaaatc caacatgaat taaatctttt gcccaatcat atcccataag ctcaattacc
    34861 ttaaaccatt gttggaaatg taagttttgt tgagaaccaa ctacatatat acacttgtca
    34921 aaatcataag tttcttttct atatattgca gcagctaaat ctcttgtcat atataacgtt
    34981 gaaccatcat tcttagtaat aagtgctggt ggcattttat actcttctaa atcaacaatt
    35041 cttgctcctt tagactcttt taaaagattt ttctcttcta atagttcgat aactctgttc
    35101 attttatcag aataaaaact ctctcctgct aaagaatcaa attctatatc taaaagttta
    35161 taaactctgt taaactcttt taaactctca tttctaaacc attgccataa ttcttgtgct
    35221 tcttcgtcac cattttctaa tttattaaac catgctcttg cttcatcttc catttctggg
    35281 tgttgttcag cttcatcatg gaatcttacg tataatttta gcaattctgg aattggatta
    35341 gactctacta cttctttttc tccccacttt ttaaaggcca ctattaactt accaaactga
    35401 gttccatagt cacctaaatg atttattctt atagtcttgt atccttgcga atcatatatc
    35461 ttatataaag cattacctat aactgtagtt cttatgtgcc ctatatggaa aggttttgct
    35521 atatttggag aagaatattc aactattaca gttttatctt ttccaaaatc actatgtccg
    35581 taattttctt tttcgtctaa aactttttta attacagttt ctgctaattg agatttattt
    35641 acaaagaaat ttacatatcc gcctaattgt attacttttg ttatctctcc acttggttct
    35701 atactttccg ccaattcact tgctatcatg tttggagctt ttctaaacac tttggctaat
    35761 ttaaagcatg gaaaagcata gtcccccata tccttatttg gtggaacttc tataagtgcc
    35821 tctatctctt ctttcgataa atcttctatt ttttctttaa ggcaatttga tattgccacc
    35881 ttaaaatctt gcattctgtt gctcctctct acattgttcc tggtttatat tgtgcttatc
    35941 tctttatgtt aaatcaaatt tcgtgttttt tcaagtattt cattacagtg taaaacactt
    36001 tagtaattat gttttctgta tatattaaaa atactataat tgcagttttt aatcacacta
    36061 ttattgattt tttataagac ttttttctat tttattgttt cttgtactat ccagttgact
    36121 ttatatactt tttttaatct tttatctatt ttcatacgtc aatgctaatt ttatatactt
    36181 ttcttttaat cttttattaa ttttcataca tcaatgttaa ctttatatac gtttttttaa
    36241 tcttttatct attttcatac atcaatgtca gctttatata ctttttttac tcttttatct
    36301 attttctata aatctttttc actcttaact tttattcctt cttcttctaa tagccttgct
    36361 gttatacctt tccctggtat ctttttacct gaaaaactac catcatatat tagattacat
    36421 ccacaagagg ggctaccctc ttttagtatt gcttcgctac atacatataa atttgctatt
    36481 tttagagttt cattagcccc tcttacaaag ttttctgtta catccttatt atctttagta
    36541 attacttttc catcaactat ctcagcaggt ggtcttggtg tagatagacc acctaactgc
    36601 tctgggcata caagtgtaaa ttctttgcct tttaaatatt ccttgacttt ttcattatca
    36661 ttattatcgc cactatattt acaatttatt cctattaaac atgcagatac caatatcata
    36721 aaaatgtgct cctatacaaa ttatatatat tctcatacaa attactaatg tattcttata
    36781 caaattattt taatttaaca attgttactc ctgctccacc ttctccatat tgaccacctc
    36841 tttgtgattt tacatgtcta tttcttctta aaatgtcttg tagccctgct tttaatactc
    36901 ctgtacctat tccatgtatt actgttacag actctaaacc tgccacatat gcatcatcaa
    36961 gatacttttc tacttccatt atagcttctt ctaggtttaa ccctctaagg tcaacttcat
    37021 tttttacact accagatttt gaccttataa tgtttctcgt tgatttagtt acatttgttg
    37081 aaacatcttt gcgtgttttt tgtaaagact taaaaggtag agtcatcttc attataccaa
    37141 tttgcactac tgcttctttt ctctttttat ctactgaaac tacactacca ttttgattta
    37201 aagttattac cttaacttct tcacctggct ttaagtcttt tatctcctta tttgatacct
    37261 ttggaactat catacttttt actgttggct gaagacttcc catagagctg gtcaattctt
    37321 ttctaagttc ttctatctta cgattctttt cttttgaagc tctttcttgt tcaagacttc
    37381 taagttcttt tattataata tctacttctt ctttagcttg tcttataatt gaaaatgctt
    37441 ctgatttagc tttttcaatc atcttatctc tttgactgac caatttttca agcttttcgt
    37501 catactctac tttcagtttt tcaatctctt cttttaatct ttcagcttct tctctatctt
    37561 ctactgcttt gattctattc ttttcaacat tctgtaacac atcttctaaa gctatgtttt
    37621 ctgtatttat atattcttta gcacgtgaaa tcacataatc acttagccca agttttctag
    37681 aaatctcaaa cgcatttgat tttccgggaa cacctataag taacttatat gttgggctta
    37741 aagtttctat atcaaattca actgctgcat tttctacacc tggtttagta agagcataat
    37801 ttttaagctc actatagtga gtggtagcta tacatttagc tcctacagaa tttatatctt
    37861 ctaatactgc tattgcaagg gcagctcctt caacagggtc tgttcccgct cctaactcgt
    37921 caaatataac taaagagtct gctgttacgt tttgtaatat agatactata ttcgtcatat
    37981 gagatgaaaa tgttgataga ctctgttcaa tactctgctc atctcctata tctgcaaata
    38041 cattatcata tacacacata ctacttccat aatcagcagg tatatgaagt ccactttgtg
    38101 tcataagtgc aaatagacct acagttttta ttgtaacagt cttaccacct gtgtttggac
    38161 ctgttatcac caatgtatgg aagtctcttc ctaaataaat tgtatttgct actaccttct
    38221 ttttatccag cagtggatgt cttccatttt ttatgtttaa atatttatcc tcatttaaag
    38281 ttggttctat tcctctcatc tgtatagaaa gttttccctt tgaaaatgca aagtccaatc
    38341 ttccaagtat ctcttgatta gaaattaagt cttcactaac ttctccaacc atagcagaaa
    38401 gctctgataa aattctttct atttcttctt gctcacctag ttttaactgt ctcaattcat
    38461 tgttcatctc tactatagtc attggctcta taaaaagtgt cgcaccagaa gatgactggt
    38521 catgcactat accagctact tgcgacctat attcagattt tacaggtact acaaatctat
    38581 cacctcttag agaaattatt gcatcttgta agtacttttg ataagtagtt gaacttatta
    38641 ttgaatttaa ttttgaccta atggattgat tttttttggc tattcttctt ctgatatctc
    38701 taagtgtact tgaagcattg tcagatattt ctacttcact aattatagca ttgtatatct
    38761 ggtcttctat gtccttgtat acatatagag aatttgatag agattgtatt attggataat
    38821 taaaatcttc ttcatcacta ctagataagc tatttcttaa tcttctagcc actcttaagg
    38881 tatctgctat cataatcaga cttcctgggt caagagaagc acctatataa gctctcttta
    38941 ccttatcttc tatatcatga ataccttcca accctactga acctctcttt ataataattg
    39001 attgagcttc actagtttct tctagcatag atttcacttc tacaaaatca gtatttggta
    39061 ctaaattttc tatatatttt agacctaaag aagatgaggc tttcttcttt aacaaatcta
    39121 ttattttatt gtattctaga actcttaacg atttctcatt cattttctat attcaccatc
    39181 ctcgatattt taactttttt atttaatttt aaaaatgtat gttttcaagc atttccaata
    39241 aaaactgcaa gttttatttc tttttcattt ctaaaattat tatatttttt ctcttttaga
    39301 tatattttac acatattttg tattatacta tgtatgtagc tatctatata ttatacataa
    39361 acatgcttta tttgtaatat aatctgttaa aaaatcttct ttaatatttt taaaggagtt
    39421 tattttagct tataaaaaaa aatagctact tatatagata aattaaggag gaacatgagt
    39481 catgggaaac gtattagaag gattaaaacc agaaagtgtt tttaaaaact ttgagaaaat
    39541 atctcaaatt cctagaggtt ctggaaatga aaaaggcata agtgattttt tacttagctt
    39601 tggtaaaaat ttaggtcttg aaactattca ggatgaaagc ttaaacataa taataagaaa
    39661 gcctgctaca aaaggatatg aaaattgtcc aggtgtagtt cttcaaggac atatggatat
    39721 ggtttgcgaa aaagaaaaga atgttgaaca tgattttctt aaagacccta taaaattaag
    39781 aattgatggt gacatgatat atgctactgg aactactcta ggagctgaca atggtatagc
    39841 tgttgctatg ggtatggcta tattagaaga taatacttta gagcatcctg cactagaagt
    39901 tttagttact gttaatgaag aagatggtat gaatggtgca gatgcactag accctagctt
    39961 aattaaaggt caatatatcc taaatatgga ctccgaggag gaaggatact tattagttag
    40021 ctgtgctggt ggtaaaactt gtgttgttag tttacctgtt gaatacaaaa aagttgaagg
    40081 tgacaaacaa ggattattag ttgaggttac tggactatta ggtggacact ctggtatgga
    40141 aattgtttta caaagagcaa atgctaacaa agcaatagct agagtattaa gtgtattgaa
    40201 tgttgattat gaattagctt ctgttgatgg tggtactaag cacaatgcta tacctcgtga
    40261 agctaaatgt gtaatagctg ttaataaagc tgatgttgaa tctgctaaaa aacaaataaa
    40321 tgacatttta actgcattta aacatgagtt tactacttct gaccctggca tgacttactc
    40381 agtagctgaa acttctgtag acaaagttct tactaaagat tgcaaagaaa aagttgttca
    40441 aatgtcttgc ttaactcctc atggagttca atcagtaagt cttgatatag aaggattagt
    40501 tgaaagttct actaacttcg ctataattga aactaaagaa tctactattg aatttttaac
    40561 ttctgttaga agttctgtta tgtctattag agatgaaatc gctgatagaa taagactttt
    40621 ggctcaagct ttaggtgcta attacgacct tatagctcaa tacccagctt gggaatttaa
    40681 aaaaggctca aaattagaaa aaatatgcag tgaaacttat gagaaattaa ctggtaaagt
    40741 tcctactgtt atggctctac atgctggtct tgagtgtgga ttattacttg ataaacttcc
    40801 tcatgctgaa gctatctcta taggaccaga tatgtttgat gttcatactc caaatgaaca
    40861 tgttagtatc ccttctgtag ctaatgtttg ggattatgtt attgaaatat taaaatctat
    40921 gaaccaatat taaatttaag ttttgattct aagtagattt taattttaag taatctatat
    40981 aaaattaact ctgtaaatta atactactgt tagtataata tttactttca atattaactt
    41041 atagaaataa agcctaatga tagataataa catgtatcat taggctttat tgtaatttaa
    41101 atattattaa gctttattct gttttaaaat taattcttat actattttaa atctatttct
    41161 tccttgtctc ttagaatcat aaagagcatt atctgctatt tgatacagtt catcatactc
    41221 ttgaccaggc tttgctataa ctacccccat actaatagat aaactttctc catttggcaa
    41281 cttaatgaga tgaatatgat tgtatacatc attacattta ttaataagtt cttccttatt
    41341 tattatatca ttgatataaa cactaaattc atctccaccc aaacgaccta ctatatcatt
    41401 ttttgcaaaa ctttcatcaa gtacctttgc aacatcaata agtactttat ctcctaacaa
    41461 gtgaccataa gtatcattaa tcgacttaaa ataatcaata tctataatca ataaagcacc
    41521 atttttttca gttgataaat tcaacaaatt ttgtgcagtt aattttctaa tatatgctgc
    41581 attataaata ttggttaaac tatccctttg agccttatct aacaatctat tcttttcctc
    41641 tttttcagaa tcaatattac atattttccc gattacatac attggtattt ttctatcatt
    41701 ataaattatt tttgaagtaa ctctaatcca atgaaaagta tcatcaagca taatagaata
    41761 catttcagtt gttccatttt cttgtgattt tattacatca aaaaactttt tattatattc
    41821 tttccttatt ttatctcttt cattttctat attgccattt tcactttcct tatcattttt
    41881 atccaaacca taataagtct caataccctt ttctggcata gaaattatca atttatcttg
    41941 cttataatca tactcaaaaa agtattcact tgtcatttca tacaactgag tatgtttttt
    42001 gagttctaaa gatatcttct cgttcatact tgtacgagta tgcaaaccaa atgccataaa
    42061 tccaataata caaattaata aactggctac aataaaaata gtctcaattg gattctgttg
    42121 cataatataa agcaacgaga agtcttgtct atatgtagta ttttggtaga caattgattg
    42181 cattttttct gatgaaatac ttataatagc tttattaaat atattcaata attcttgttt
    42241 atctttttta attataccaa aacaaatact ctttgcttta taagtttgag gaactagacg
    42301 cacattttta tacttaggtt gattcatata gtactgcata gaataacctt cgccataagt
    42361 gtaatctgca tagcctttat taacagcttc tatacactct tctaaattat cataccaaac
    42421 tatattctct tcaaactgac cataataatt aatacctttg gctagagcta cacgttttcc
    42481 atctaaacta ttctcattta atttattatt aatcatcata gtatattgag tagaaagaaa
    42541 aggccgagac ataactatat tatgttcacg agagtattca taatcataaa tcattcctgc
    42601 tacaatatca atttcacgct ttttagtcat gttttctaaa tcttcttgag attttacagg
    42661 cacaagttca aattctaagc ctgttttatc acatatatga tttaacaaat caattgatat
    42721 acctcttaat tcacctgtat ctttatctat attttgaaat ggtggtttgt ttaaaagtac
    42781 tcctactttt aatttttctg tctctttaat gtaatcagtt tcttcatcag ataaaaacat
    42841 ttgattgttc tctggtgtaa agtatttttc atataaagta gttgaaaaat atgggtcttt
    42901 ctgttcaata ttattaattg ctgagttaag cttttgagtt aaatttgtac tacctttagg
    42961 agtaatgaaa caaaatggtt ttgatgaaaa ttttgctaca gtacgtacac cttctagtgg
    43021 gttcatactc acattaagaa gtacatctgc tttaccactc tttagtgctt ctagttgctc
    43081 atcatttgtt ttacaatata ccaattcagg tgacataaga ttcatcttac aatactcatc
    43141 taactcttta agcctagtat cagaattttc tattacagca atacggattt ttttcacatc
    43201 ttgtgaactc attatgttat caatatctgt attttcatat aatgcttgta gtactgtatt
    43261 tgctactcca taattattcc ctgcataatt atatattttt tgcattggct cattgtataa
    43321 catacctccc attatatcaa tttcaccatt ttctaacatc ttcattaatt ttgtaataga
    43381 ttcatttata tctccaggaa cttgaacaaa ttcataattc catccagtat actgtgcaat
    43441 ttctagtaaa tattcatatg tatacccaaa atagttacct tgctcatcta tttcagttaa
    43501 acctttttga ataggataag caacacgaat ggtacgatta tcattaccta tagcgttgat
    43561 accaaaagaa aatatgcaag aagtgaatat agatgtaatt acaataatta ataaaagtaa
    43621 tttttttaaa ttcactacac aatcctccat atagttaaga tttacagtat aaattactgc
    43681 ataaacttgt agacatgtaa aaatagatta gtatataaaa ggatatataa atctacttag
    43741 aaatttaaat atccttaata tatacaatat atttaaatta taactcattt atatgtctat
    43801 tacaacaaat ttaccctgct aaatacctac tctatatccc ataattttac aagttgtagt
    43861 gaagtttaag ttctatctca cctcaaattt agcagtgttt ctatttacag ttcttttata
    43921 tttcacatct ggataaccca atataaatga agtgataaca gcttcgttct cgctcattcc
    43981 taacagttct tttatctttg gctctacatt tgatgccata acaaaaaatc cattataaca
    44041 aattcctaat ccttgagaat aggccataag ttccatatta gatgcagcca gtcctccatc
    44101 tacataagca cttccaccta gagacatatc tcctacaact accataagta gtggggcatc
    44161 ataaaataaa gaatcatttc cgttctcttt atatcttttg tacattttct taaatgtatt
    44221 tttatagata gaacgtacag ggtcattgtc atcagtatct aatgccaaat tataaagacc
    44281 ttgtattgcc agttctttca cttcttttag cttttctttt actaatatgt atcttatagg
    44341 ctgtctatta ccacctgttg gtgtgtatct accagcttcc actatgtttt ttattttttc
    44401 ttcttctacc tctatatctt tatattgtct tatacttctt ctaaatttaa taaaattcaa
    44461 taacttttcg ggttctaatt caaaactttc acgattgtat tctattactt catttttatc
    44521 aaatccttgc atttctattg catctgttgg gcaaattgcc atacagtgac cacagttaat
    44581 acagagaata tttttaacct ttgctttatc atccttaact tttattgcat ttacaagaca
    44641 gtctgattca cacataccac agcctacaca tttttctaaa tttatgtcta tcattccaaa
    44701 ttattcctcc ttttaaaaaa tttactttag tataaaacaa atagttgtct aagtcaattt
    44761 ttattttttt aacaatatac tagccttaac tacactttaa tggagaaaaa tatacataaa
    44821 ttggaactta aattagaact tattcaaagt aaaagataaa agaagtagat gtctaaaagt
    44881 gaacttttta gttaattaga tattctttat tgtatgaaat caagtggata tttatcattt
    44941 caacaataaa aaaggcttac ctctattttg gatatgtccc tgtcaagtag acagactaaa
    45001 aaaaggctat ctttatgcac tgagatagcc ccttttccca actactattg tttatcttaa
    45061 tattagtcaa taaatttaat ttgctataac ctgtaagtta cattttaatt tgatgtaact
    45121 ttaaattaca tttttaagta atatctttaa attatattct taaaatatat cattaaacta
    45181 cattttaatt aatctaaaaa ctatatctta attaatctaa gaaacttaca ttataatctt
    45241 ttagtttccc attatcatat acaatctttg cactaaagat atttccagtc ttagcccaat
    45301 ttaagaacag cctgtcacct tcccacatat ttaaatcgaa tattttatca ttatcaaccc
    45361 aaattaaatt tccttcttta caatcattta aatctttttc agataattca ccttcaaact
    45421 catctgagaa atacaaaaac atataccaat cttcttcatc tttaaatttt ggaaaagtta
    45481 tcaacccttt atatgacaat gatttaagag taagaccagt ctcttctttt acttctctaa
    45541 ttacacattc ttctggggtc tctccttgtt caaactttcc tccaactcct acatacttac
    45601 cttcatgtat atcatctttc tttttatttc tgtacaacat caaagtcttg tcatcttttt
    45661 ctatataaca aatagttgta agtttcatgt atctcgcctt gctttctctt tatattctca
    45721 actaattatt ttcagcactt tcaagcacat cacttatatt cttcattgca ttaagagaaa
    45781 gctctgcaaa ttcatcaaat ggaatcccta tatcttctat acttctcatt gcatctctat
    45841 ttacatttga agcaaatctc gtttctttca ttcttttaat aacagactta gtctttacac
    45901 tggtaagttt tttatctgga taaactaaag ctatagcagt tataaaccca gttatagggt
    45961 ctgctgcata tattgctttt tccattaggc tagttcttgt aactcccatt ccatcatgat
    46021 gaccaagtat tgctctatac atttcttcgt caccgaagtt ttctttttta agcagttcta
    46081 ctgttgttgt agcatgtcct tcacatattt ctcctggctt tcttccaact atatctgaat
    46141 ccaaatcatg taaaagacct gccactgccc actcttcttc tttttctggc tctagtcttt
    46201 tggctaattc tctcataaca gcttcaacag cataacaatg ttttctcatt ctatcagttt
    46261 ctaaatactt aaataaaatt tctaaagctt cttctctcat tttcaccaat ccccctgttt
    46321 tttcacatat atgatatttt tatatattaa tacacataaa ttcaagtttt tatatatatt
    46381 catgtatatt tttcatgtta tatctattct acacctttat agtagtgtcc tgcaactgtt
    46441 ccaatttcat ctaatttctt atacaagtct gtagcctttt ttccaagttt ataattatta
    46501 ctcaatacct caatgtgagc ttcaataacc tttttaacca tctcactatc ttcaactgta
    46561 aaatctatcc taaatacatc tattccaatt tcctctaact catatagatt gtccaacata
    46621 caagtaacct tcgaattgta aattgtactt ctacagaata tatcttgtgt tatcctataa
    46681 cttgcatccg taaagtctct taaagcataa ctactttcct tacatttagc aactctcttg
    46741 tcctttttac aatctctaac aactactccc ataggacagt attctgtaac catcatagga
    46801 gcatatccat atattactgc ttcaatttct acatctgtat attttaaagt ttgtttaatt
    46861 tcatttaagt ttagttcttg tgacaaacaa actgtacttg ctccctcttc tttgaagtgg
    46921 tttatacttt cactattgaa tgcatttaag tagcaatcta tatttaagtc ttttttctta
    46981 aagtattcca tagagcccca gttaccaatt tgaaccttat taattccttt tatattatcc
    47041 aatatctcaa attgcttgta ctctttattt ctaacaatcc ttggtgctga atagattaat
    47101 tttttgttag tgactgctgt attatagtgg ttcttattca aagtatctat tttacttgta
    47161 tttgacaaag tatctatgtt atttgtattt gaacccatta gttctttagg agtatctaaa
    47221 gacgaagaag tatctaaatc catagaaata cttgaatcta tagaaatatt tgaatctata
    47281 gaaatacttg agaccatttg taaagctttt gataatgtat ttatatcttc ataatagatt
    47341 aaatctacat tataatttaa tactgcttct aattgctcca agttcttaac tttaactcta
    47401 atcttaggaa ctgtatcaag ttttttctgt ttatttactc tatttggctg gtattttaat
    47461 ctattcttct taaattttct atcttttatc tttattcttt cttcatctaa aagctcaata
    47521 caatcccttc tcatttgatt taacacactt ataggcatac ttacaccttc atccaggtca
    47581 atttctatct cttttaactt atatggtgta tttccaagct tttgaatttg tgtttctatt
    47641 ttttctttac ttaaagccac ctttatagct ttttcaacta ttttgtctcc ttctaccacc
    47701 gatttatttc cacaaaaatc acttataaaa agctctggct ttttacctag tttaatactc
    47761 acttttgcac gtatattagt ttttatgaac tctttatctt gctgaaatgt agcttgtact
    47821 ctatccatca attcgctgtc agaagtctta tatataagct gatttctctt tgcttctcca
    47881 acaaaatcta attctatcaa atccccttta tgtgctattg tagttatctc tccatccttt
    47941 attattcttc caatagtacc tccacctaag ttaattccat cacctttctt aagcgtattt
    48001 tctagcatta ctctaagtct tttgttcttt ctattataat ctaaaacttt tccaatatat
    48061 agcccttgat tattaggtct attagagttc attatatcct taccaacttc acctaatata
    48121 tatccttttg taaactttct attaaatatg gtatacaaat tatttattat ttcctcagaa
    48181 atacttattt ttttcttatc tacaaattca ttaatcgctt ctctatatcc tgaaaccaca
    48241 gtagctacat actctggctt tttcattcta ccttcaattt ttaaagataa tacacctgct
    48301 tcaattattt tatccaaatc ttctatagta cttaaatctc taggactcaa caaataatct
    48361 ccattacttt ttactacttc tcctgtattt atatctatca actcatactt ttgtctacat
    48421 ggctgtgcac acctacctct atttccagac ctattgccta tcatactact cataaggcat
    48481 tgacccgagt aacatacaca taaagcccca tgtacaaaaa cttctatatc tacagttgta
    48541 ttttcacaaa tccattttat ctcatcaaca tttaattctc ttgctaaaac aactctatca
    48601 aaccctacac tctgaaggta ttttacatct tctaaggaat gtgctaccat ttgagtgctt
    48661 gcatgtagtt caaagtcagg taatagtttt tttataagtc ttgccattcc tatatcctgt
    48721 actattaaag catcaatatc tatatcgtat aaaaatttta catactctac aaaatcttct
    48781 atttcatact gttttatcaa tgtatttaca gtaacaaaga cttgcactcc ccttatatga
    48841 gagtatttaa cagcctcaat cagctcattt ctatcaaagt tatttgcaga tgctctagca
    48901 ctaaagtctt taccacctaa atacacagca tttgctccgt tttgaactgc tgcttttaat
    48961 gaatcaaaag agcctactgg tgcaagtaat tctatttttt tcatattttt ctcctctatt
    49021 tatacttgtt aggttaatat aattatatct ataacttaaa tatttgtatt aaatttatat
    49081 atttcttact atattttaaa ttcttcaatc aatttatata ttagttattt atatttggtt
    49141 catttgagtt aacatataca ttatttacta tatttagtat atttctatta acttatatat
    49201 ttattactgt attttaaatt cttcaattaa tttatatact gcttactaaa tttaaaacat
    49261 tttaatta
//
LOCUS       DAKBNN010000006        47052 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000006 DAKBNN010000000
VERSION     DAKBNN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.006"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            38..382
                     /locus_tag="OUB02_000350"
     CDS             38..382
                     /locus_tag="OUB02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCT8884164.1"
                     /translation="MEIKRYEGTGRMSRTVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(541..2637)
                     /locus_tag="OUB02_000351"
     CDS             complement(541..2637)
                     /locus_tag="OUB02_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCT8884165.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPLIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPFEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTVITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(2650..3810)
                     /locus_tag="OUB02_000352"
     CDS             complement(2650..3810)
                     /locus_tag="OUB02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HCT8884166.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(3829..4896)
                     /locus_tag="OUB02_000353"
     CDS             complement(3829..4896)
                     /locus_tag="OUB02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HCT8884167.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            5248..5814
                     /locus_tag="OUB02_000354"
     CDS             5248..5814
                     /locus_tag="OUB02_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884168.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(6033..7019)
                     /locus_tag="OUB02_000355"
     CDS             complement(6033..7019)
                     /locus_tag="OUB02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HCT8884169.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(7049..7744)
                     /gene="pxpB"
                     /locus_tag="OUB02_000356"
     CDS             complement(7049..7744)
                     /gene="pxpB"
                     /locus_tag="OUB02_000356"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCT8884170.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(7764..8984)
                     /locus_tag="OUB02_000357"
     CDS             complement(7764..8984)
                     /locus_tag="OUB02_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCT8884171.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(9013..9804)
                     /locus_tag="OUB02_000358"
     CDS             complement(9013..9804)
                     /locus_tag="OUB02_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HCT8884172.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            10392..11021
                     /locus_tag="OUB02_000359"
     CDS             10392..11021
                     /locus_tag="OUB02_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8884173.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(11135..12649)
                     /gene="glpK"
                     /locus_tag="OUB02_000360"
     CDS             complement(11135..12649)
                     /gene="glpK"
                     /locus_tag="OUB02_000360"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCT8884174.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            12893..13435
                     /locus_tag="OUB02_000361"
     CDS             12893..13435
                     /locus_tag="OUB02_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8884175.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSYISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            13449..15941
                     /locus_tag="OUB02_000362"
     CDS             13449..15941
                     /locus_tag="OUB02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8884176.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            16362..16760
                     /locus_tag="OUB02_000363"
     CDS             16362..16760
                     /locus_tag="OUB02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8884177.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(16977..17186)
                     /locus_tag="OUB02_000364"
     CDS             complement(16977..17186)
                     /locus_tag="OUB02_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884178.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(17218..17427)
                     /locus_tag="OUB02_000365"
     CDS             complement(17218..17427)
                     /locus_tag="OUB02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884179.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            17726..18145
                     /locus_tag="OUB02_000366"
     CDS             17726..18145
                     /locus_tag="OUB02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884180.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            18373..18828
                     /locus_tag="OUB02_000367"
     CDS             18373..18828
                     /locus_tag="OUB02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884181.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(18984..19796)
                     /locus_tag="OUB02_000368"
     CDS             complement(18984..19796)
                     /locus_tag="OUB02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT8884182.1"
                     /translation="MAEQKYYTLLTKAGKASIANATALGKKVDLVKLQLGDGGGSEYS
                     PTEEQTSLKEVVWEGNISNVKIDDDNSNWIVIETVIPGSIGGFMIREVGIFDSENKLI
                     AISKYPETYKPTADSGSVKDLIIRIILVVSNTSSVNLKVDPTVILATLKDIQELDKKI
                     DTTKITLTSGIETTKIELNTKIDNTKTDVTSKIGDTTLLNTTDKTSLVNAVNEVKEGL
                     DSIETTAEKTSYDNTVSKLAATNVQGAIDELCTSVNTARISLINDIKDMLPI"
     gene            complement(19798..20415)
                     /locus_tag="OUB02_000369"
     CDS             complement(19798..20415)
                     /locus_tag="OUB02_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCT8884183.1"
                     /translation="MKLIDKLPSFYKNYMVEAIQDSYDSELSSLKENIDDTINQMFVN
                     TSTWGLDMWESILCIQNNEDLNYETRRSNIKAKMRSLGTTKLKVIKNICEAYTKTDVE
                     VTVLSNEFIFILEFIVNNCSYNSIVELDKVLENVKPCHLEHKFKMILLNKNELFCGTA
                     INTGETVTVYPWTPSNIEIFGEITISTGNDRSMEKVILYPKQEAI"
     gene            complement(20415..21464)
                     /locus_tag="OUB02_000370"
     CDS             complement(20415..21464)
                     /locus_tag="OUB02_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCT8884184.1"
                     /translation="MYSNQTYETIKNRTLKNINLDVYKGEGSFLNDMVSGNTFELSKM
                     YVELSNIYKMAFIEDTYNQLLDKRVNEFGVYRKLGTEATGEVEFIGENGVEISNGTII
                     SYKDLSFVVIKDIVIGGEEGNISPVEALEIGTRYNLPSDCEFKVNDNIFGIEKITNIN
                     KFSGGTEIETDEELRERFFRVQKNQATSGNKAHYEEWALEVDGVYNAKVYPRWNGAGT
                     VKVLLFGQNNQAVEEEIITKCREHIEEEMPIGATLTVTTPIPLNITISASMELESGYT
                     LDMVKNSFIENINQYFRSITGEIVYTKIMGILISTSGVHDLSNLLVNNSTDNITIEDD
                     KIPSVTTLDFSEVEV"
     gene            complement(21457..21888)
                     /locus_tag="OUB02_000371"
     CDS             complement(21457..21888)
                     /locus_tag="OUB02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HCT8884185.1"
                     /translation="MSNTIFPFIGVPEDYEIPKKEELGLFREVAWNFEKDEPIVENGD
                     FKIIEKNEAIKVWVYKCIKTNKNEHEIYSNDYGTELTDLIGQKYSKGLTESEASRYIK
                     EALLMNPYILQVDVVNTNFKKDILGAYIKISTIYGEVEINV"
     gene            complement(21888..22214)
                     /locus_tag="OUB02_000372"
     CDS             complement(21888..22214)
                     /locus_tag="OUB02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HCT8884186.1"
                     /translation="MANSINEFIGIIRKEGKYYNKPSFFVGKIINKLPDLKVSMNNIV
                     LDKDNLLIDNWLFDRATKSFIITQSNNHTHELNESLTNQLDKGDTVIMFKVGEKFAII
                     SKLVSL"
     gene            complement(22228..23745)
                     /locus_tag="OUB02_000373"
     CDS             complement(22228..23745)
                     /locus_tag="OUB02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT8884187.1"
                     /translation="MIINRSKDSSSVNINFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFADNKLAIGNIPKTNVKYTKMFISVNGYDTIMSAYTEASKTTKKKYMIEANLDKFN
                     IIEKGTVTLNIIFEEGFNLINTTFSESMENVKNKVLLVDQYGNKISEKVNNSLFKEVN
                     VIMQKVIQQQENSTTDIENEFKGIERTCSLKGYGDTSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGDYQIELELNFQNIMDEKSVGQDEQKEEISDLSGNGALNGREVKAEFT
                     AYYPSNDPMEGGLYQKMDGKKLVPSNNTCAAPEVLKFKTQIQVKGTGTNIDGKTYTVT
                     DRGGAIKVVNGIYKIDILMSSKKECYNFGRRKGTIIIGDGTGYTNSTGKVKELISIAK
                     GKKGCKYVWGASGPNTFDCSGFTQWCYKKIGINIPRVSRAQGSVGKQVEKKSLQPGDL
                     VFFSSKGPKGTIDHVGILIGNNEFIHSPHTGDVVKISSLKGSYYSKNYVGARRFL"
     gene            complement(24014..24436)
                     /locus_tag="OUB02_000374"
     CDS             complement(24014..24436)
                     /locus_tag="OUB02_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884188.1"
                     /translation="MEMWLRQAEDRFRFPVIPPSISINGKSTINSSNILKVGEIATFG
                     GVALKSINISSFFPNKEYSFCNYKGFPSPYDCVNKIQRWMNEGLILRFTVTETNINME
                     VIIEGFSYEERDGTRDVYFSLDLREYKRLQILRANPKK"
     gene            complement(24436..26766)
                     /locus_tag="OUB02_000375"
     CDS             complement(24436..26766)
                     /locus_tag="OUB02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884189.1"
                     /translation="MGNVREEGINMYITDNYTPKMNQIISVTEALKRVTVSVSPSVNT
                     MANSIKSSMASASSSINSINSSIKKMTSSIKSLSNSIEKMNGSMNNSGSSNKDFSEGL
                     ETLGRSAENARSKLVRLSQVANSKKGRDTHKNVKAKNNESKNNKSDKPEKIKTVQDIV
                     KRVKTQTVDTSKKIVTGKLPELKNKVFNSNLVMGVKGNNIVQNTLNILNKIKNSKWIS
                     SISVKDLAKNVLIGTLSKLKQFAGRMWTGAISIKDKASSILGGIKDKISDITNGFSIG
                     GAVKKGVDLVGKGQNQKAVLTNVVQRTTGVKDKIEAKSEANNYYNDVIKDIALKSPFD
                     PSEVISMSTKAMTISGGKIDKSKDITQAMVNVRALNMETSSDQDVSAAFVSASKGNMD
                     ALNTLVGENYKTLDDALEGIKEKQNGLANEMAGTIPGLLSSASTYIDMGLNSMIEPFD
                     SILEGGLKKINSFLEKVPSGIGGFSQKVADVMNGKKIAENKQNPIETQVATALGHVPQ
                     SIGNTSQMLVQNIDFTALTNSLLPVVNLVNVLLDSINNKSPVAQGIISIFGTVVTTVF
                     QLIGPVVEAVSPIIMRIFTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIE
                     PILGAFFNILDKVCKIVKDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAG
                     TKAGDSGKAYSSKKGNNAFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNIN
                     IAKLADTIVIREEADIEKITSKLVASIQVAQLGGVL"
     gene            complement(26766..26933)
                     /locus_tag="OUB02_000376"
     CDS             complement(26766..26933)
                     /locus_tag="OUB02_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884190.1"
                     /translation="MYYYWKKKGIRPSLFYTMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGI"
     gene            complement(26960..27406)
                     /locus_tag="OUB02_000377"
     CDS             complement(26960..27406)
                     /locus_tag="OUB02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8884191.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(27520..27948)
                     /locus_tag="OUB02_000378"
     CDS             complement(27520..27948)
                     /locus_tag="OUB02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCT8884192.1"
                     /translation="MANMEARNVMSGTWGELWLDGDKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(27962..29026)
                     /locus_tag="OUB02_000379"
     CDS             complement(27962..29026)
                     /locus_tag="OUB02_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCT8884193.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIINNDIKKVIIDDYIGRVPNSYDNKCILI
                     VAIKEYLEELEKEELIEVGSLVDIDIEAQKQYLKSKNIDISNMKEQEIREANTGSKVF
                     LKAKIKMLDAMEDIDLSIEI"
     gene            complement(29031..29474)
                     /locus_tag="OUB02_000380"
     CDS             complement(29031..29474)
                     /locus_tag="OUB02_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884194.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(29723..30163)
                     /locus_tag="OUB02_000381"
     CDS             complement(29723..30163)
                     /locus_tag="OUB02_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884195.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(30268..30465)
                     /locus_tag="OUB02_000382"
     CDS             complement(30268..30465)
                     /locus_tag="OUB02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884196.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            30634..30954
                     /locus_tag="OUB02_000383"
     CDS             30634..30954
                     /locus_tag="OUB02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCT8884197.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            31036..31542
                     /locus_tag="OUB02_000384"
     CDS             31036..31542
                     /locus_tag="OUB02_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884198.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            32243..32998
                     /locus_tag="OUB02_000385"
     CDS             32243..32998
                     /locus_tag="OUB02_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT8884199.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(33141..33569)
                     /locus_tag="OUB02_000386"
     CDS             complement(33141..33569)
                     /locus_tag="OUB02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCT8884200.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(33990..34190)
                     /locus_tag="OUB02_000387"
     CDS             complement(33990..34190)
                     /locus_tag="OUB02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT8884201.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(34373..35542)
                     /locus_tag="OUB02_000388"
     CDS             complement(34373..35542)
                     /locus_tag="OUB02_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCT8884202.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            35778..36599
                     /locus_tag="OUB02_000389"
     CDS             35778..36599
                     /locus_tag="OUB02_000389"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HCT8884203.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIHIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(36675..38063)
                     /locus_tag="OUB02_000390"
     CDS             complement(36675..38063)
                     /locus_tag="OUB02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HCT8884204.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(38198..38956)
                     /locus_tag="OUB02_000391"
     CDS             complement(38198..38956)
                     /locus_tag="OUB02_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HCT8884205.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVGYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     ASIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(38958..39674)
                     /locus_tag="OUB02_000392"
     CDS             complement(38958..39674)
                     /locus_tag="OUB02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCT8884206.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(39676..40383)
                     /locus_tag="OUB02_000393"
     CDS             complement(39676..40383)
                     /locus_tag="OUB02_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT8884207.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(40499..41035)
                     /locus_tag="OUB02_000394"
     CDS             complement(40499..41035)
                     /locus_tag="OUB02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8884208.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(41137..42207)
                     /locus_tag="OUB02_000395"
     CDS             complement(41137..42207)
                     /locus_tag="OUB02_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCT8884209.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHDEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(42200..43282)
                     /locus_tag="OUB02_000396"
     CDS             complement(42200..43282)
                     /locus_tag="OUB02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCT8884210.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDLNKDINEDSYNNDYYNREEESIKETDEYN
                     DDSINFEAIYKDLEDIENKKGEDKHE"
     gene            complement(43269..43601)
                     /locus_tag="OUB02_000397"
     CDS             complement(43269..43601)
                     /locus_tag="OUB02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT8884211.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            43800..44921
                     /locus_tag="OUB02_000398"
     CDS             43800..44921
                     /locus_tag="OUB02_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HCT8884212.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(45049..46947)
                     /locus_tag="OUB02_000399"
     CDS             complement(45049..46947)
                     /locus_tag="OUB02_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HCT8884213.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        15534 a         7903 c         5324 g        18291 t
ORIGIN      
        1 ttttaataga ttttttaact atggaggtat ttaaaatatg gaaataaaga gatatgaagg
       61 aactggaaga atgagtagaa ctgtagttca taacaataca gtttatttat gtggacaaac
      121 tcatgctgaa ggaggagtaa ttgaacaaac aactgaagtt ttagctaaaa tagaagattt
      181 attaaacaaa tatggttcag ataagcaaca tttgctatct gttactattt atttaagaga
      241 tatgaaagac tttgaagcta tgaactctgt ttgggatgct tgggttgaaa aagggtttga
      301 acctgctaga gcttgtgttg aagctagact tgctagagag catttgttag ttgaaatgag
      361 cgttgttgca gctacaaaat aatcaattta gcttatagga ggctatctca aaacagagag
      421 ataagcctct ttttcattgt tgaaatgatg aaaatatatt tgatttcgta taaaagaaag
      481 tgtctcacga actaaaaaat tcattttgag acacctcctc actatttaat ctatataaat
      541 ttacttattc tctattggca atatcaattt agatggatat ttacctccat ggtaaatcgt
      601 attctttgct tctatatatt caatactatt atatccctct gtcgtgttgc tattttggaa
      661 aataaagttt ttagcactag atgttatatc tagtctaatt ttatgaccaa ctttaaacgt
      721 gtttgatatt tttgaagtta taaccgtaaa cttataaatc tctccttctt ccataaactc
      781 tgacttataa aaactatttc taaatctagc actaagtata ccatcagcta atttaattga
      841 atttccatct aaatccacgt ccataatttt tataacccag tcagtatctt tggcagaact
      901 tgatgcaaaa aactctaact ttatatcacc tgttacagta aattcttcac taaaagcatc
      961 tgatgtataa cacaacatat cacttctttt ttctaaatct ttgtagttat ttggaactcc
     1021 aacttcattc tctgacatat caattagttg tactgatggg tcttttggat tatatatata
     1081 actatcatac ttttcttcta aatcattatc aaatactaat ctaccatttc ctaaagatgt
     1141 gttagcattt ccatcactta ttagatacat acttttatct atcttattta ttattggcca
     1201 attttcttct gtcttccact tattagaacc tacagagtaa tactctactg gagcagtcgt
     1261 atctatgtta ttttctatac cttttaagta tttatcaaac cataatagat aattataatc
     1321 caaatcatat cttaatgaac tatttcctaa agaaatacca tttatatctc ttaaagtatt
     1381 tgaattatgc atccatggac ctagaatagc ctttctcttt cctttttcat agtcctttat
     1441 aacatctaag gcctctgtag ttcccatact attatcatca taccacccag atacaaccaa
     1501 tgcaggtaca tttattttat ctttttgtaa atgccaatcc atcaaatccc aatactcatc
     1561 tttttctact ctctttaacc attcttccag aaattcaatt ctatatccaa gagcttcaaa
     1621 aggtatattt tctattggtc gtatgtttaa aacatcatcc caatcatctc ttaccatttt
     1681 gcttctatca actttatttc ttgataccat aaatgcccat gcaagcatac cagatacaaa
     1741 agctcctcct tttcttggta tatcaataaa agggcttcct gatgttacta tactaactaa
     1801 agctttaaga tgcttattgc ctgaagatgc agctgcccat tggacataac caagatatga
     1861 tgctccaagc attccaacta ttccacttga ccattcctgt gatactatcc aatttatggt
     1921 actatctcca tcttccctct catgaatcaa aggttcccac ttaccttctg attcatttct
     1981 tcccctaaca tcttgtatta caacagcata tcctctttgt acatatttat aatatatttc
     2041 tttatcattt tcttttccat atggagttct cataagaatt gtcggtgctt ttttggtaga
     2101 atctacaaaa tcagggagat atacatctgt agaaagtttt ataccatctt tcatatcaat
     2161 catataattt ccaccaaatc ttatagaata tatctcttca tttttatgag ttttttccca
     2221 ttcttttaat acagtatact cttcatatcc atccataatt aatatagtac aagtttctct
     2281 ggataaacag ataaaaccta ctattttatt atctacaata attatatcta gtgggaactt
     2341 tttgtttctt tgaacccata tatattcatt aagtttagaa tgcgtttctc ctaagttatt
     2401 tataactgtg tctttatctg tgtttactaa gcttttagta tatacatcaa agttattaaa
     2461 taaagacttt aaatctattt ttgtaagatt taaatagtat gtattttctt ctttgcttat
     2521 tggttttaaa ctttctattt tatttgtaaa aggattcaac ttttcaatta ataaatctcc
     2581 atcaaagatt acatttccac tgtatagtcc agatatgtat aatttaaatt ttatcaatta
     2641 aactcccccc tataatccta gagacatctt tattgcatat gccactgata tacctgcata
     2701 atttcctaat actccaccaa ttactcccat tacgactgct acacttacaa gcgaattcca
     2761 ctttgcagaa gcagctacta aagctgaagt aggaccatca gcaattgctc ctactataga
     2821 aagtattaca tattgatact ttatctttaa aagtctagtt atgcctaaat ggaaaacaaa
     2881 tgaagcaaaa attatgctaa aacagtaaag tgttatagta aaagttgaac ctaaaaactc
     2941 ttttatatct actgaaaaac ctattgtaca taaaaagaat aatgcaacaa ataatcctaa
     3001 atctaaattt ccctttagtt tctttactgg ctttaattga gcaattataa ttgaaattgt
     3061 tgtaactaaa agtattcttg ctgcactact aaaacttgaa ttgaaatatc cagcaagtat
     3121 tgttgaaact tctgtaacaa caaaacctat agccaacatc caagctatat ctttaatact
     3181 ccattctttt gactccataa attcttcatt ttgacaatct tctaattctg attcttctac
     3241 atgatatggc caaagttttt taaacttctt tgaatttttt aatattgctg gtagagcaaa
     3301 cataaatatc aatgttggta ctccaattac atagtctgct gcatttgctg ctgcaagagt
     3361 ttgtctactt acattaagac ctgttccaat agctgtaaga ttagcacttc caccagtgta
     3421 tgtacctaca aacattccag caaccttcca accttctgat attttttctg caaatactaa
     3481 tccaaataaa aatgccatga tacttacagt taatacagct acaaaaattg caattaaagg
     3541 ttctcttgat aattttgtca tttcttttaa gtcaacactc atcaacatta tagtcataga
     3601 aactggaata gcatatgttg aaatagttcc atataactca gtacttactg gaactacttt
     3661 taagtttgaa agtattattc ccattatgat tattgtaagt gctggtccta agcttttaat
     3721 ggctttgtat ctttgtaagt aaaaacccaa tgcaatcatt gatgtcatta cagcaagtat
     3781 actacctgga tttgaaaaaa gtgtattcat atttaacctc caaatttatc ataagtttac
     3841 ttctattcct aaacctggtt tattgacaag attcataata tctccatcca ttacaaatcc
     3901 tccagatata ccttctaatt cttcacaaaa cataaaacta tcaaggtcag cttctgttat
     3961 gttcttttta gatgctatta aatttgctgc tgctgttata ccaattctcg tttccagcat
     4021 acatcctaac atacaattta ctccacttgc ctcagctatg ttgttgattt ttatagcagg
     4081 atatattcca cttgatttca tcagcttaat attaaatgta tcaactgcat tcttttttat
     4141 agccttaatt gcatctattg gtgaatgtaa tgattcatct atcataattt ttgtgttact
     4201 tttatttctt atgtaagcag taccatctaa atcccaatgt ggaagtgctt gttcaattgc
     4261 atcaacatta aattcttcta actttttgat agtatttata ctgctattta cgttccatcc
     4321 ttgatttgca tcaattctaa ttcttatact ctctccaacg gcttctctaa ttaactttac
     4381 agcttcaata tcgtcttcag gatttatacc tgctttaagc ttcaatatcc taaaaccttc
     4441 ttttactctt tctaaagctt ctactgccat tttttctggc ttatctatac ttatagtaat
     4501 atccgtttgg actttactat caaatcctcc aagaacttta taaaggggtg cattcataat
     4561 ttttccttta atatcatata aagctatgtc aatagctgct tttgcagaag tattacctat
     4621 aataattcca tccatgatgg tatgtatggt ttcaatatct agtgggtctt tgcctattaa
     4681 ctctttttta aacataagta atactgaaag aacattatcc aaaacttcac ctgtaactgc
     4741 tgcaagaggt gctgcttcgc caaatccaca atacccttca tcagtttcaa tttttaaaat
     4801 tacactttct ccatattcaa ttactccaaa tgaaactact acaggttttt ttaatttaat
     4861 tctaagcttt tcaaatttga tatctgttat cttcatatta ccctccttat tttttgaagt
     4921 tataattcaa tttttaaaat tttatacttt aaattatatt attacttcaa tatatatgtc
     4981 aagataattt actgaaaatt tactcttttc caaaatataa tgtgataatc ttaattcaat
     5041 taatactctt ctattaaatt tttttattat tattttcttt gtattaattt aacaataatt
     5101 cattttcata gacttttatt tggatttaat taaattttaa ttgtcaaaat ctactatgta
     5161 ttcttattgc cactacttct atgttgtgct ataattactt tgttttaagc gaatatttta
     5221 tattaatttt attatttgga ggttcttatg ctaggtgaaa agatgagaaa tataagaaaa
     5281 agcaagaaaa aaacattaag tgatgtttcc aaattgacag atttatctat aagttatatc
     5341 tctcaaatag aaagagatgc tattgaacca tccttgtctt cattgagaaa aatcgctgaa
     5401 gttttagaca ctcctttata tatgtttatg gatgacaata atactgatga tttagtcatt
     5461 agaaaagaag atagagttat gatgaaattt ccaaagagtg aaatgtttta cgagatagta
     5521 tctccaatgc ctacaactga gtttacacct tcaattttat ttgtagagtt tgaacttaaa
     5581 ccagaaagtg aggatgccaa agattatata tatcatgcat cagaagaaat tatggtggta
     5641 acgagtggtg tggttgatat ttgtatgggt gaaaaggtag ttagattaaa tccaggtgac
     5701 tcaacattta taaaagctaa tgtaccacat aaaatcatca atccaagtaa agatacaata
     5761 gctcgtggat atggagttat atcacctcca atttggccaa taaaatctaa ataaactcaa
     5821 tatgacacac ctactttttg tagataatat aatagattta aatattgctt ttattttaaa
     5881 tttgaagtat ttatttaaat ttaaaatatt tactcaaact aaaaaaatag ctatttgact
     5941 aatctaaacc atcttaatta tggtaaatag taaatagcta tttgtaagtt atattataaa
     6001 ttataataaa aattgtattt gttaaaatta aattaaactg ctatatcatt taatactttt
     6061 gcctttacaa ctcttatatt tttcatactc ttgtagatat cttgaatctt atcttcttgc
     6121 tctctcaata gtctatgtgc ttcatatata tctacaagtt caaattttac tacatcattt
     6181 ggctttgctt gtgctaactt atacaaatct acacttataa cgttccctat cttagtatag
     6241 ccaccactag tctgtctatc tgataacatt ataatcggtt ttccatgacc aggtacttgt
     6301 atagctccat aagaaatacc atcagatata atatctgctc cattaaggtg ctttatactc
     6361 tctccatcta gtctacaacc cattctatct atttcattcg taactttgta tttatttgat
     6421 aaaaatttct ctacttcacc gttagaaaaa gcatcaaatt gaggaccttt tacaattctt
     6481 agtacgattt ctgaactata agttggaata tattttgaac ttaatttttt atttattgta
     6541 aaatcttttt cctgaataaa agtatttata taatctcctt ttttaagtgc tctaccttca
     6601 aatccaccaa ttttagctct tgtataagtt gacttacttc ccattgcaag tggaacatct
     6661 attccaccag ctattgacac ataggctcta gcacctgatt tcacacctct aaatgataat
     6721 acatcacctg gatttatttg atatgacctc cagttttgaa tttcttttcc atttatttta
     6781 gcccctaaat ctcccccagt tactgctatt tgcgtatatt catcaaaggt aattgtagga
     6841 cctaacatta aaacttctaa agaagcctca tcttcagaat ttcctactaa tatatttcct
     6901 actctatgtg catactcatc cattgctcca catccagata caccatattg accatagcca
     6961 actctgcctc tgtcttgtat taatgtttgg aaacctccta attcaacttt gaatcccata
     7021 tataccactc cctgtactat aacgatattt aaatacttaa gttatttttt tcgatttcta
     7081 taaattcatc taatgttatt ggaacaaatt ttataaaatc tccagctcta agcaaaatct
     7141 gttcttctct attttcatca tataatttta aaggtgtcct accaattatt tgccatccac
     7201 ccggactatc aataggatat acaccagtct gggaacctgc tattccaaca ctcccaccat
     7261 aaattttagt ccttggcact tctaatctag gtgtagctat tctttcatcc ataccaccta
     7321 gatatggaaa tccaggagta aaccctagca tatatatcaa gtactctcta cttgtgtgta
     7381 tttttataac ttcatctaca gtgagattat tgtagcttgc aacgttttta atatctatac
     7441 caaaaacctc atcataacat actggaatct catgtatttt aggttttggt aatactatac
     7501 tttccaaatt atcttctatt ttttttaaat tctcaatcaa actatcaaaa tctatttcta
     7561 atggattata acttatcata agtgacctat atgttggtac catttctgtt acaatacctt
     7621 taagattcga tatttctatt gctctcataa atgaaattac ttttttgttt atatcttctg
     7681 atatctcatt tccaaattca gcaaccactg ccttatcccc tgatatcaag tatctagttt
     7741 ccataaatgt cacctcttct aagttaagca aataaagcac ctaggctgct aagagaagtt
     7801 attcctgtat atgcagatat taaaactact attaatccaa atattaaaag ccatgtagga
     7861 tgcttgtaat ctccaacaat atcttttctt ttagatgcta taagcataat tcctaatgtg
     7921 attggaagta tcaatccatt taaagctcct gctaaaatta ataaagtagc tggttttcct
     7981 ataaatgcca ttataagtgt tgatattgct ataaatgcta ttataaaata tttttcatat
     8041 ttatctataa aaggatttag tgtctttaag aaagaaactg atgtataagc agctcctata
     8101 actgatgtaa ttgcagcaga ccataataca agtccaaaga atttatatcc tattgctcct
     8161 gcactaaatt taaaagctga agcagctgga ttttctgggt caagttgaag accttttgaa
     8221 accactgcca gtattgctaa aaataaaaga actctcatca ttgtggcaac taatataccc
     8281 aaaagagaag atttagttat ttcttttata ttttcttctc ctgttattcc accgtctatt
     8341 aatctatgtc ctccagcaaa agttatataa cctccaacac ttcctccaag tagagttatt
     8401 attgggaata tcagtccttt tggattttct ggttttacca ttcttgatac tgcctctcca
     8461 acaggaggat gatttgatat tgctacatat ccaactataa ttatcattcc taatgctaaa
     8521 aatttagtta tcttatcgac tagagaattt gaattttttg acataaatac aaatatggct
     8581 attaatccac ttaatacagt tcctaatgtc atattcatat taaacattac attcatacca
     8641 agtgcagctc caccaacatt tcctatatta aaagctaatc ctccaagagc tactaaaaaa
     8701 gctactaaat aacctaatcc tggtattatt ttatttgcta tatcctgacc tctaagtcct
     8761 gatacaccaa ttactctcca tacatttact tgagccccta taaaaaggat tgttgtaatt
     8821 aaaattacaa atgaaaaatt cggtccaaaa tcttgggtaa attgtgcagt ctgagttaaa
     8881 aaccctggtc caattgctga tgttgccatt ataaatgcag cccctataag tgctccaaca
     8941 tcttttttat ttttagtttt atctgtaata ttttttgtac tcatatttta tctccccctc
     9001 atgttatcag tcttatacta tgttctctga cgaacaaact tctatgtttt ctaatggaca
     9061 aacttctata ctttctaatt caaaacgctt tcttatttct ttgacaaact ctattgcttt
     9121 tggattgtcc ccatgtacac atatagaatc tgctttgata tgtataactt cacctgttaa
     9181 agtttctaca gttccttctt ttaccatcct aacaacccta tctattgcat gttttgtatc
     9241 atgtataact gctccttcta cacttcttgg aaccaaaaaa ccattattat cataagctct
     9301 atctgcaaat acttcatttg caaacctaag tccaatttct ttagcagaat ttatcatttc
     9361 actattatat agccccaaca gtataatatc tttatcgact tcatatatag cttcacaaat
     9421 ccccatagct aactctttat ctttagctgc catattgtat aatgctccat gtggttttac
     9481 atgttgtata ttacatccat ttgaacttgc aaatgccatt aaagcaccca attgatattt
     9541 tgtataatct ttagcttcct gctttgttat tttcatctca cgtcttccaa atcctattaa
     9601 atctaggaaa cctggatgtg cgcctatttt cactccattt tttttagcta gctgtactgt
     9661 tttttccata tgactagggt cacctgcatg aaacccacaa gctatattgg ctgatgaaat
     9721 atattttaaa acttcttcat ctaaaccaat cttatacgtt ccaaaacttt ctcctaaatc
     9781 actatttaaa tctactttat acaatttatg acccctcctc gctaacattt tgatagaata
     9841 agtagtttta atttttaaat tttgtttatt ttatatattg catgaaatat tttatcactt
     9901 gtattcaatt ttatttttgc aactattcta ttgcatttaa ttaatataat tttaccattt
     9961 aaggaatatt tttgcaatta ttttaattga acattcattt gtatttcttt ttcaaaaaat
    10021 ctttttacca ttttttagtt aataattaat tattttcact taaaataagg gccttaacct
    10081 tcatttttaa agtatttttc atttttaatc actttatatg caagacagaa tcatttttct
    10141 tcattcattt gtttctttca tctatataat tatcattctt tgtaagtaaa atatctttat
    10201 atttttataa ttaattcttt atatagtttt aagtataata aaatcatgta tactaaatgc
    10261 atgttaaaat attattttaa aacatgcaca atatttgtac atattgtaga catataaacc
    10321 tataaattat acaatagtat tgttaagatt atatattttt ttaacataaa ttttgtattg
    10381 ggggaaaaac aatggataaa aactatacga tgccaatata ccaaaaaata gcgttggata
    10441 ttgcaaataa aatatacacg ggtgaaatac aagaggattc agtactattt ggacgctctg
    10501 ttttggctgg taaatacaat gtatctccag aaacgataag aagagctgtc aaaattttag
    10561 aggacattgg cgttgttaaa agcataaagg gtaaaggtgt tattgtacta tctccagaca
    10621 aagcttcttc ttttataaaa aaatatagag atattacaaa tatatcatca tataaatcaa
    10681 cactttataa tttaatagat actaaatcta atttagaaaa tgaaatatta gatactataa
    10741 ataaaatttt agactattct aacagacttg aaataatcaa tcctttagtg cccgttcagt
    10801 tcaccataaa ttctaattgc aaatatattg gtcaaacagc tgcacaaact aaattttggc
    10861 aaaatacagg tgctacaata gttgcaatta aaagaggtga agaacttata atatctcctg
    10921 gaccatatat tgaattttta gaaggagaca ttctcttagt agttggtgac caacacattt
    10981 acaattctat accaatgttt ttatatgaaa acgaaaaata aattatataa tttagtgtga
    11041 ttatataaaa atgcaagatt aacaacaaaa aaagagcttc atattttaca taaagctctt
    11101 tttaaattca aataaattta actttgatta atttttaaat atccaattca tcttcttttt
    11161 cccagcttaa agctctcgat acagcttttt tccatccttt atataattta catctcttct
    11221 cttcactcat atttggatta aactcttttt ctactgacca ctttgaagta atttcatttc
    11281 ttccaatata gaatccaaca gacaaccctg ctaaataagc agctcctaaa gctgtagttt
    11341 ctactatttt gggtctatct atatctacat ttaatatatc tgattgaaat tgcattaaaa
    11401 aattattatt acttgctcca ccatcaactt ttaatgattt tagttttaat tttgaatcgt
    11461 tttgcattgc ttctaaaaca tcctttgtct gataagcaat tgattccaat gtggctctta
    11521 ctatatgttc tttttttgct cctcttgtaa gtcctaaaat tgaaccccta gcatacatat
    11581 cccaatatgg agctccaaga cctgtaaatg ctggcactac atatacacca ttagtatcct
    11641 ctaccctatt tgcatacatt tcactatctt ttgcagattc aattatcttt aattcatctc
    11701 taagccattg aacagatgct cctccaataa atatacttcc ttctaatgca tatgttactt
    11761 ttccatcaac tccccaagca attgttgtaa gtaacccatg cttagattta actatgttac
    11821 tgcctgtatt cataagcaag aaacaacccg ttccataagt attttttgca gtaccttctt
    11881 caacacaatt ttgaccaaat aatgctgctt gttggtctcc agcacatccc gcaataggta
    11941 ttttagctcc tgataacatg cgctcatctg tatgaccata aacatagcta gaaggtttta
    12001 ctaatggcaa catgcttatt ggtatgtcca attctcgtaa tatatcctca tcccattcta
    12061 aatcatttat attgtacatc attgttctag atgcatttga gtaatctgtt acatgtactt
    12121 ttccacgagt taaattccat attaaccatg tatctatagt tccaaataat aactctcctt
    12181 gttctgcttt ttccctagct ccttctacat tatctaatat ccattttact tttgttgctg
    12241 aaaagtatgc atctattaaa agacctgttt tttcttttat tgattcttcc aatcctttat
    12301 ttttaagttc atcacatatt tctgacgttc tcctacactg ccatactatt gcgttataaa
    12361 taggtttacc agtataccta ctccaaacaa ccgtagtctc tctttgatta gttatcccta
    12421 ttgaagcaat ttcttctggt cttatacctg ctgtttctat aacttctctc attaccccac
    12481 tttgtgaccc ccatatttcc ataggatcat gctctaccca tccagctttt ggataaattt
    12541 gattgaactc tttttgtgca attttcacta tttcgccttt tttattaaat agtatagctc
    12601 ttgaactggt tgttccttgg tctaatgcca ttacatactt cttttccatc accatattct
    12661 ccttatcata taatatttaa tttattaaaa agaaaaattt aatatcacat atttcaataa
    12721 ataatcatac ttaagtacaa atttaatata atagatattt agtaagaaga cacttatttt
    12781 ctatcatatc tatataataa tattatacat tatagcttac atattgatta ataaatttta
    12841 aaggagtata ataaacagtg ttcataataa ttttaataaa agaggtggta atatgccaaa
    12901 gattttagaa aatgtaaaag aagatatatt aaaagtgtct cgggatatga ttttagaaga
    12961 agattactcg tatattagca taaggaaaat agctcaaagg tgtggtataa gtgcaggtac
    13021 agtgtataat tactttaatt caaaacaaga aataatagaa tatatcacaa aatctgaatg
    13081 ggatttattg ataaggcgaa ttgaatactc caataaaaac actgaagatt acataaaaaa
    13141 actaaacatt atttttactg aaataagaaa ttttataaac aaagttcata atatccaata
    13201 caatgacttt ttaaacactt ttgaaacaga gagatttttt gaaatgaaaa aacacaaaga
    13261 cgactttcat gaacagcttt caaataaagt atatgaagct ttagaaaaag aaagtttttt
    13321 taataatgac aaattagtat gcaatatcat aataaaaatg tttttttcat attcaacata
    13381 tcttcatata gaatttgaag atttaaaacc ctatattgaa aaattaatac attaaggtgg
    13441 tgtttacctt gaatcataat actagtatta agaacaagga cagtaatcca aaaatatttt
    13501 tagtacttac actttatcta ttaggtatat ttatgggagc tattgatact ggaatagtat
    13561 caccagcaag aacgattata caaaatagcc ttggagtaaa tgaaaaaacg ggaatttgga
    13621 tgattaccat atacacatta acatacgctt ctgtaattcc tatctctgga aaattagcag
    13681 ataaaatagg aagaaaatat gtgtatctag ttagtatatt tctatttggt agtggttcat
    13741 taatatgtgg attatcatca ctattttcca atttctatat tttattaatt ggtcgtgtta
    13801 ttcaagcaat tggtggtggt ggaatcatgc caattgctac agcagaattt ggaactacct
    13861 tcccagaaaa taaaagaggc atggctttag gtttagttgg ggcaacatat ggtattgcca
    13921 atattttagg ttcaagcatt ggtagcacaa ttctaagtat ctttggaact caaaattgga
    13981 agtggctatt ttttgtaaat cttccaattt gtttaatcat acttattggt ggagttttct
    14041 gtattaaaaa caataagtct gaatctaaag aaaaaataga taagcttggt acattgatgt
    14101 tgatatgtat aatcgtatca ctattatatg gtcttatgaa tatagacttt tttaatttta
    14161 aaaattctat acaagatata tcagtttatc catacttatt gacatttatt gtacttatac
    14221 ctattttcat atttattgaa aatagagcta aagaccctat tttaagcttt gaatattttt
    14281 taaatccgag aatattgata gtacttattt tatctttaat tgttggtatt ggaatgatgg
    14341 gtatggtatt cgttccacag tatgctgaaa atgcattaaa gataaatgct ggttctgggg
    14401 gatattttgt agctatattg ggattatttg caggtattgc tgctccttta agcggaagac
    14461 ttattgacaa atttggggca aaaaaaatac tattactagg attttctgta tctatgatag
    14521 gttctcttta cctagtttta attgctctaa aaaccaatac tgtatttagt gtatgtgtaa
    14581 gtttaatatt tatgggatta ggaatgggat ttactatggg aactcctcta aactatatga
    14641 tgttatcaaa tacaagatta gaagaatcta attcagcact tgcaacatta tccttgatac
    14701 gctcaattgg tacttcaatt tctccagcta taatgattgg atttatagca catgcaggat
    14761 tatctgtgca agataatatt atggacttag ttggaaagcc ctcaactcct aaaatagttc
    14821 aacttgaaga acttgaaaac atgatagatg acttaaaatc agaccctgaa atggcaaaaa
    14881 atttaaaaaa tgtctctatc ccaaatatga atgatacttc aaatataaat atggacatga
    14941 caggtggcaa acttccaaaa tccttactta ataaagttca gtcagcagat acaaccaaca
    15001 ttaccaatat aacaaaagaa atatcaacaa caatgtttga tgaaaaagtt ccatctataa
    15061 ttactaaaat acagaaaaat gtacaaaaag gaatagatgg aacacaaagt ggaataaacg
    15121 gtattgaaga tggtgaaaat aaactaaata gtggtattaa aggagttcaa actggtattg
    15181 acaatatggt aaaagctaga gctggtatta atcaaggaat agatggtgta aaaaaaggta
    15241 ttgctggtgt tgacaaaggt ataaaaggta tggaacaagg tcttgaaaaa caagatgagg
    15301 ccatcaagga acttacaact gcttataata aaatacctac tacagaaaaa actcctactg
    15361 ataacgaaaa taactcaaat gaacaaaacc agagtaatac cagtaattct gaatcagatg
    15421 acactaagaa tgattctaca gaaaataaga ataacaattc cagcgagaat aaaccaaatg
    15481 taaatgaaca aaaagaaagt ttaaatgctc aaatacagca actaaaaaag acaagagatg
    15541 acttaaatac aaagttacaa aaaaataaaa ctcaaaagaa cgaattaagt aaaaaactaa
    15601 gatcaatgga atctcaaaag aaagagctac aaagcaagtt aaataattct ataagccaaa
    15661 agaaagacat ggaaaaaagc cttgatacaa tgcaacagca aaaaaaatca ctccaagcag
    15721 ttttagcaaa gacacaagag gtaaaaactg aaataccaaa ggcttttgat aaatcaaaat
    15781 tggattatat aaattccata gaaaataata gaacaaaaat agaaaataca ctacaatcta
    15841 ctctaaactc aggatttaaa cagatgtata taacagtatt ctgtgtaaat ctactagcat
    15901 ttataatatt attgttctat aaagaaaata aaactagata aaagagtgtt atagtattat
    15961 attgtaaatt tatcttattc ttaaatattt tattctaaag aataaattca taaaattttt
    16021 gtaattgatt aatataaatt acaatataat actatttttc tcttaatctt tttctctttt
    16081 acagacaatc aaaaagcaat cttagtttat cgaaataaat ttatacaatc tattcataca
    16141 cccctttaca tttttataac aattaaacct attgttatat atacaaatca aaataaacca
    16201 tatattttca actataatat gttaaaatca tattatgagc atattaattt aagcaaaatg
    16261 tcatttagac tttaatttag tagttttata ttataaattt atatattttt aattttaaat
    16321 gtcatttaga cattactcat aaaaagaaag gagtgtattt tgtggatact ttaggaaaaa
    16381 gaattgctta tttgagaaac tccaaaaaac ttactcagcg caaacttatg gatattttaa
    16441 aatttgaaaa cttaggcaag tatgaaacag gagatagaaa acctaattgt gatatactaa
    16501 tgtcaatagc tgattatttt aatgttacga ctgactggct tctctatggt aaagagaagg
    16561 taaatgtcaa tagtagtgta aaagaagata aagaagatta cttacatgtt actaatgatg
    16621 aaatgatgat attaaatcta tacagacaac taaacgaacg tgataaaata aaaatagaag
    16681 gaatacttga acttaagata tctgaatata aagacttaaa aaagcattcc tctaataata
    16741 atgaagataa aatggtttaa acatacataa tttgatacga ttttacatca tctattaacc
    16801 ttcttttttt gtagtatatt actaaaatat ggatattgga ttatttaaaa acaagtatag
    16861 tctaatttca agcacattta taatatttag attatcttat ccatatttta gtttaagtac
    16921 tttgaaaaat ataatacatc tctatgtata acttagattc ctaagaaatg cattactcat
    16981 gtttttaatt cttccaacat ctttctatac acaattgtac atccttcata attcccctct
    17041 ttaagaagga ttgaacatgt actagaatat ttagttaaag tatttaatct ttccatattt
    17101 aagcttttta tattactgag tgctgtaata aaatcatcaa ttgctctaca acactctata
    17161 accccatctg aatccaattt acacacaaga atattcacct actttatata ctatttatta
    17221 atcatcttct gaatattcta tattaagtaa ttgataaatt gatgatttat attttttacc
    17281 tgttcttctt ccatttaaaa tagctgacaa atagttttca ttaacaccta ttttcttcgc
    17341 taattccctt tgagtcatat ttaattcaat aagtctttgc ttgacttgca ctcccaaaaa
    17401 aggtatccat ttttttcgag catccattta tcaacttcct ttctaagtga tttatattta
    17461 cttttatatt attttagctt acacaccccc tctatttagt atatattttt aatatgtaca
    17521 catattaagt atataaattt atttaatact aaataagatt ttttgattac tttgtgctaa
    17581 aattaggatg taaattcgta tcattaagtt aattataaag tcattttgac cttaagtcaa
    17641 tggtttttat gtcattttga cctttaattt gaaactattt acatcttaaa aagtcttttt
    17701 gacattaatt gaatggagag tgtttatgga tactttagga gaaagaattg tttatttgag
    17761 aaaagcaaaa aaccttaaac aatatgaact agaagaaatg ttgggctgtg acaacttaag
    17821 taaatttgag agaaacatta gaaaaccaaa ttatgaaata ttaaaatcta tagctgagat
    17881 atttaatgtt tcagtagact ggctattaaa tggagataat ctatcacata aatcagattt
    17941 aatttgcgat tcctcctcaa actatccact taatagcata aactctaatg aaataaaact
    18001 tttaaacaac tttagaaagt taaatgatta tgataaagcc aaaatagaag gtatgattga
    18061 attaaaactt catgaatatg aaaaagagaa ggatttagga aaaattgagt ataacaaaaa
    18121 taaagatgag aaaatagata aataattttt atgttgcttt tgatacgatt ttacatcatt
    18181 tctttgttct aatattttta tttcatttat tttctttttt ataattttat gataaaataa
    18241 ttagtatatt ttgctttact tgaatgtaat tatttataca aaagtcaaac ataatataaa
    18301 tgtagtaaat ttttagtttc aagtaatatc tcgttattat aactaaatat ttattaaaat
    18361 taggaggata aaatgggctt aagagacaaa ttcgcgcaat catttgctag atcaaaaact
    18421 atgagtggcc ctgagaaaaa ggctaatgaa ataatgggta aattgcttct taagaaagct
    18481 atattaccta tcgttttaat gttcgtgata ataatcgctg gagctatgct taaaatcaat
    18541 tcttgggtta cacttggcat aaatttagtt attgctgttg gtgctttctt ctacataaga
    18601 aattctagta aaaagtatca gaactttaaa ccttatgttg gcaatttgat tagtttagag
    18661 aaaaaaggta aaaaagagta cgttgctatt ataaaacaag gtaagttacc agttaagtta
    18721 caaatagctt atggtggtga agaccttgaa catgttaaga aaaatcaaat ggttcaaata
    18781 agttataatc cagatgctaa aatagctatt ttggttaata gacaataatt taataaaaaa
    18841 aaagagttgt cttaaaattg atttttcaca atcaagttta agctcaactc cttttttaat
    18901 ttttatatgt tatttttatt acttttttat taatatctct aagttataaa attccagtct
    18961 atacaatatt ccatttttta tttttatatt ggtaacatat cttttatatc attaatcaaa
    19021 ctaattctag cagtatttac actagtacat aactcatcta tagctccttg aacatttgta
    19081 gcagcaagtt tacttactgt attatcataa cttgttttct ctgctgttgt ttctatgcta
    19141 tcaagacctt cttttacctc attgactgca tttacaagac ttgttttatc agttgtattg
    19201 agtagagttg tatctcctat tttactagtg acatctgtct tagtattatc tattttagta
    19261 tttaactcta ttttagtagt ttctatacca cttgttaatg ttattttagt tgtatctatt
    19321 tttttatcta actcttgtat atcttttaaa gttgccaaga tgacagttgg gtcaactttt
    19381 aaatttacac ttgaagtatt agaaactacc aatataattc ttataattaa atcttttaca
    19441 cttccagaat cagcagttgg tttataagtt tctggatatt tagatatagc tataagctta
    19501 ttttctgaat caaatatacc aacttctctt atcataaaac cgccaatact acctggtatt
    19561 acagtttcta taactatcca gttagagttg tcatcatcta tttttacatt acttatattg
    19621 ccttcccaga caacttcttt aagagatgtt tgttcttctg taggactata ctcacttcct
    19681 ccaccatctc caagttgtag tttaactaaa tctacttttt taccaagagc agttgcattt
    19741 gcaatagatg ctttacctgc tttagttaaa agtgtatagt atttttgttc tgccaattta
    19801 tatcgcctcc tgtttaggat ataagattac tttttccata cttctatcat ttcctgtaga
    19861 aatggttatc tctccaaata tctctatatt tgatggtgtc cacggatata cagtaacagt
    19921 ttctcctgta tttatagcag ttccacaaaa tagttcattt ttatttaaca aaatcatttt
    19981 aaatttatgt tccagatgac atggctttac attttctaat actttatcca actctacaat
    20041 agaattataa ctacaattat taactataaa ctctaatata aatataaatt cattgctgag
    20101 tactgttact tcaacatcag tctttgtata agcttcacaa atgtttttta taaccttcaa
    20161 tttagttgtt cctaaacttc tcattttagc ctttatattg cttcttctag tctcataatt
    20221 taaatcttca ttattttgaa tacacaatat actttcccac atatcaagac cccatgtgga
    20281 tgtattgaca aacatttgat ttatagtatc atctatattt tcttttaatg aagataattc
    20341 actatcataa gaatcttgta ttgcttcaac catataattt ttataaaatg atggtagttt
    20401 atcaattaat ttcattaaac ctccacctca ctaaaatcta aagttgtaac actaggtatt
    20461 ttatcatctt caatagttat attgtctgtt gagttattga caagtaaatt gcttaaatca
    20521 tggactccag aagtacttat tagaatcccc atgattttcg tgtatacaat ttctcctgtt
    20581 atacttctaa aatattgatt tatattttct ataaaactat tttttaccat atctaaggta
    20641 tatccacttt ctagctccat agatgcactt atagttatat ttagtgggat tggagtagtt
    20701 actgttagtg tagctcctat tggcatttct tcctcaatat gttctctaca cttagttatt
    20761 atttcttctt caaccgcttg attattttgt ccaaacaata aaaccttaac tgtccctgct
    20821 ccattccatc ttggatatac ttttgcatta tatacaccat caacttctaa agcccattct
    20881 tcataatgtg ctttattccc acttgttgct tgattttttt gtactctaaa aaacctttct
    20941 ctcagttctt catcagtttc aatttctgta ccacctgaaa atttatttat atttgttatc
    21001 ttttcaattc caaatatatt atcatttacc ttaaattcac aatcagatgg taaattatat
    21061 ctagttccaa tttctagtgc ttcgactgga cttatatttc cttcttcacc accaatgaca
    21121 atatccttta ttaccacaaa tgataaatct ttataggata taattgttcc atttgatatt
    21181 tcaacaccat tttcacctat aaattctact tctccagtag cctcagtacc caattttcta
    21241 tatacaccaa attcattcac tctcttatct aataattgat tataagtgtc ttcaataaaa
    21301 gccattttat atatgttaga aagttcaaca tacatttttg aaagttcaaa ggtatttcca
    21361 gaaaccatat catttagaaa agaaccttct cctttataca catctaaatt tatattttta
    21421 agggttctat tttttattgt ttcataagtt tgattactat acatttattt ccacctcccc
    21481 atagatagtg gatattttta tatatgcacc taatatatct tttttaaaat tagtatttac
    21541 tacatctact tgtaaaatat atggattcat aagcaaagct tcttttatat atcgagatgc
    21601 ttcactttct gtaagacctt tactatattt ttgacctatt aaatcagtca actctgttcc
    21661 ataatcattt gagtaaatct catgttcatt tttatttgtt tttatacact tatataccca
    21721 aacttttata gcttcatttt tttcaataat cttaaaatct ccattttcta ctattggttc
    21781 atctttttca aagttccatg ctacttcacg aaataaccct aattcctctt ttttaggaat
    21841 ttcatagtcc tctggaacac ctataaaagg aaatattgtg ttagacatta taaactcacc
    21901 aacttactta ttattgcaaa tttttctcct actttaaaca taataactgt gtcaccttta
    21961 tctagttgat tagtgagaga ttcatttaat tcatgagtat ggttattaga ctgtgttatt
    22021 ataaagcttt ttgttgctct atcaaacaac caattatcta taagtaaatt gtctttatct
    22081 aaaactatat tattcattga tacttttaaa tctggcaatt tatttattat ttttccaaca
    22141 aaaaaggaag gcttattata atatttccct tccttcctta ttattcctat aaattcattt
    22201 attgaattag ccactatacc acctctctta taaaaatctt cttgctccta cataattctt
    22261 agaataatag cttcctttta gactagatat tttaaccaca tcaccagtat gtggagaatg
    22321 tataaattca ttgttcccaa ttaatatacc aacatgatct atagttcctt taggtccttt
    22381 tgaggaaaag aaaactaaat ctcctggttg caaacttttc ttttctactt gctttcctac
    22441 acttccttgt gctctggata ctctaggaat attaattcct atttttttat aacaccactg
    22501 agtaaaccca ctgcaatcaa aagtatttgg tccacttgct ccccacacat atttacatcc
    22561 ttttttaccc ttagctatgc taattaattc ttttaccttt cctgtggaat ttgtatatcc
    22621 tgttccatct ccaataatta tagtaccttt tcttcttcca aaattataac attctttttt
    22681 actagacatt aatatatcta ttttataaat accattaact accttaatag ctcctcccct
    22741 atctgttact gtatatgttt ttccatctat atttgtacca gtgcctttta cttgtatttg
    22801 tgttttaaac ttaagcactt caggagcagc acaagtattg ttagatggta caagcttttt
    22861 gccatccatc ttttgatata agccaccttc cattggatca tttgatggat aatatgctgt
    22921 aaattctgct tttacttctc ttccatttaa tgctccatta ccacttaaat ctgaaatttc
    22981 ttctttttgt tcatcttgcc ctacagattt ttcatccatt atattttgaa aattgagttc
    23041 taattcaatc tgataatctc cattttgcca tgtatgttta tctgtatcta tatagaataa
    23101 tcctaccaat tttgtatacg aatcttttac ctttacacct ctaccagtta tacaacttgt
    23161 atctccatat ccttttagag aacacgttct ttcaattcct ttaaactcgt tttctatgtc
    23221 tgttgtacta ttttcctgtt gttgtatgac tttctgcatt atcacattta cttctttaaa
    23281 taaagaatta ttaactttct cactaatttt atttccatat tggtcaacta ataatacttt
    23341 attctttaca ttttccatac tttcagaaaa agtcgtattg ataagattaa atccttcttc
    23401 aaagatgata tttaatgtaa cagttccttt ctcaataata ttaaatttat caagattagc
    23461 ctctatcata tactttttct ttgtagtttt gcttgcttct gtgtaagcac tcattatagt
    23521 atcatatccg tttacagata taaacatttt agtatatttt acattagtct ttggtatgtt
    23581 tccaattgca agtttgttat ctgcaaatac ttgctttgca atatcctcta ctaacttatc
    23641 cttaaaatta taggacactt cactttgtaa aagtaaaaat cccatatcct tggctgtaaa
    23701 attaatatta acactgcttg aatctttaga tctgttaatt atcatccctc taaaaagttc
    23761 tttttcatct acataaaagc aaactgtgct tgctattgga atatcaattt gttgaaaatt
    23821 aatatcagaa gctgactgta ctatagaaaa ttctaatgtt cttgaaggag acttataatc
    23881 tccagaccaa gaaactgaat ctactatatt acttatatca tagatatttc cgttttttat
    23941 gtgtacccaa atctttataa taaaaaatca cctacctttt tctctgattt tgagtaattt
    24001 tttataaatt gtactacttt ttagggtttg cccttagtat ttgaagtctc ttatattctc
    24061 ttaaatctag tgaaaagtac acatctcttg taccatctct ttcttcataa ctaaaacctt
    24121 ctattatcac ttccatattt atatttgttt ctgtaactgt aaatctcaat attaaacctt
    24181 cattcatcca tctttgaatt ttatttacac aatcataagg ggatggaaat cctttataat
    24241 tacaaaaaga atattcttta tttgggaaaa aactagatat attaatactt ttaagagcta
    24301 cgcctccaaa agttgctatt tctccaactt taagtatatt tgatgaattt atagttgatt
    24361 ttccatttat acttattgat ggtggtataa ctggaaatct aaatctatct tcagcttgtc
    24421 taagccacat ttccattata agacaccccc taactgtgcc acttggatac ttgcaactaa
    24481 ttttgatgtt atcttttcta tatcagcttc ttctctaatc actattgtat cagctaactt
    24541 agctatgttt atatttccac cattttttct gcttctatat tgatttgctt cttgtttagt
    24601 taaaaccatt tcaccttcat gaagtctggt ttgatagtca ttataaggaa catagttcaa
    24661 tccaaatgca ttattacctt tcttacttga ataagcttta ccagaatcac cagcctttgt
    24721 tccagcatat ggattatctt tgtaattttt tttactcttt tccactagat ttaaaactgt
    24781 tcctgtgatg cttccatttt ttatcttatt aatcatagtt tgccaccatt tgcatatatc
    24841 tttaactatt ttacatactt tatctaatat gttaaaaaaa gctcccaata ttggctctat
    24901 aattttccat ccagtttcca acagaggtcc taaggtctcc catacagttt tccaaataac
    24961 acccagtgtc tctataaaat tgtttatttg aggtgcgtat tctcccaaaa aggtaaaaat
    25021 tctcataata attggactaa cagcttctac aacaggtcca atcagttgaa aaactgttgt
    25081 tactacagtc ccaaatatac ttattatacc ttgtgctact ggtgatttgt tatttatact
    25141 atctaataat acattaacta gatttactac aggaagtaat gaatttgtta atgcagtaaa
    25201 atcaatattt tgaacaagca tctgacttgt attacctatt gattgtggta catgtcctaa
    25261 tgctgtagct acctgagttt caattggatt ttgtttattt tcagcaattt ttttcccatt
    25321 cattacatct gcaacttttt gagaaaaacc acctattccg cttggcactt tttccaaaaa
    25381 tgaatttatt ttttttaatc caccttctaa tatgctgtca aaaggttcaa tcatactgtt
    25441 taaccccata tcaatatacg tggatgcact agataataag cctggaatgg taccagccat
    25501 ttcattagct aatccatttt gcttctcttt tatcccctca agtgcatcat caagtgtttt
    25561 ataattttct ccaactaaag tattaagagc atccatatta ccttttgatg cacttacaaa
    25621 agctgctgat acatcttgat cactactagt ttccatattt aaagctctga catttaccat
    25681 agcctgagtt atatctttcg atttatcaat cttacctcca ctaatcgtca ttgccttagt
    25741 actcattgaa ataacttcac taggatcaaa agggcttttt agtgctatat cttttattac
    25801 atcattataa taattattag cttcagattt agcttcaatc ttatccttaa ctcctgtagt
    25861 tctttgaaca acattagtta aaacagcttt ttgattttga ccttttccta ctaaatctac
    25921 accttttttt acagctcctc ctattgaaaa tccattagta atatcagata ttttatcttt
    25981 tattcctcct agtatactac tagctttatc ttttattgaa atagctccag tccacattct
    26041 tccagcaaat tgttttaatt ttgatagtgt tcctattaaa acatttttag ctaaatcttt
    26101 aactgaaata gaactaatcc atttactatt tttaatttta tttaatatat ttaaagtgtt
    26161 ttgaacaata ttatttcctt taacacccat tactaaattg ctattaaaaa ctttattttt
    26221 aagttcaggt aatttaccag taactatttt tttagaagta tctactgtct gagttttaac
    26281 tcgcttaaca atatcttgta cagtttttat tttttcaggt ttatcgcttt tattattttt
    26341 agattcatta ttttttgctt ttacattttt atgtgtatct cttccttttt tactatttgc
    26401 tacttgtgat aatctaacta acttacttct agcattttct gcacttcttc caagagtttc
    26461 taagccttca ctaaaatctt tatttgatga acctgaatta ttcatacttc cattcatttt
    26521 ttctatagaa ttacttaaac tttttatact actagtcatt ttttttattg aagaatttat
    26581 actatttata ctactacttg cactagccat agaactctta atactattag ccattgtatt
    26641 tactgaagga gaaacagata cagtaactct tttaagggct tccgttactg atataatttg
    26701 attcatcttt ggagtgtaat tatctgtaat atacatattt ataccttctt ctcttacatt
    26761 tcccactata taccacctcc tgtcaaagga caaactccat atccatgctt cattttttca
    26821 acttcttctt caatttctaa ggcgaaaaaa gcttcaataa gctttaattc gcctttatcc
    26881 attgtataaa aaagggacgg tcttatacct tttttcttcc aataatagta cattatcgta
    26941 gtcttggtat ccgtccctat tagtttttta cctcttctat tactgcattt ttaccataac
    27001 ccatcacatc ttgaaggatt ctatataagt tagtaatttc acctggtaat aatagttttc
    27061 ttgccaattc ttttggtgtt ggagccttaa atttatccat tagctcctta tttttaaata
    27121 aaagctttcc atccaaatca caaacacctt ctataattgt aagcatctgc atctctggta
    27181 agtctatatc cgttttcata ttttcatcaa ttttaagaca agtattttgt atctcttcat
    27241 ctttacttat tgttaaagct ctacacatta ctttaaattc ctgcccaaat atagttgata
    27301 atctcttaag ttcaactata gtacttggtc tttctaagtt ttctgtatct gcatttaaaa
    27361 gtaaatctac tatgtttata ttttttgata atccattttc attcattatt atttgctcct
    27421 ttatcaatca aattcaatca actaactttt ttacttatta actacctgaa tttattaagt
    27481 atatatctac taaatatcag tataaaacca aatataaaac taaattatgt caagaaaatc
    27541 atactcagta aatgtgaaag gagcttctat ctctcccttt actccaacct cccaatcagc
    27601 taaagttaaa tcatcaaatg ctatattttt tactgctatt ctttctgctc cataagaatc
    27661 tgggtcattt aatttagata tagcaacaaa tctaggttca gaacctctct ttatcttttc
    27721 tccaattaac ttgtgcattc ttgaactaac atggtataga gttattgaac cttttccttt
    27781 atatcccata tactttgtat cagtacccat ttgacctgct attataatat cctcttttgt
    27841 aaattccatc tttgcttgaa acttctttac ttctgctact ttatctccat caagccaaag
    27901 ttctccccaa gtaccactca ttacatttct agcttccata tttgccatat taataatcct
    27961 cctatatctc tattgataaa tctatatctt ccatagcatc taacatcttt atttttgctt
    28021 ttaagaatac ttttgaacct gtatttgctt ctcttatttc ttgttctttc atattagata
    28081 tatctatatt tttagatttc aaatactgtt tttgggcttc tatgtctata tctacaagtg
    28141 aaccaacttc tattaattct tccttttcta attcttctaa gtattccttt attgccacta
    28201 ttagtatgca tttattatca taactattag gaactctacc tatatagtca tctattatta
    28261 ctttcttaat gtcattattt ataatatcta aagtgtcaac tatttttatt ttctggaaca
    28321 tttctccttt ttctgctgtt aattcagtta aagaatttac tcctctagca attcttatag
    28381 ctcctgcttc tttaataagt ataagctctc ctttattaac ctttgattct gcatcaactt
    28441 tcgtcatctt aggtatatcg actacatcac taagtttagt ataagttact gattgactta
    28501 aaggtgtacc tgctataagt ccagccaccc tacttgtaaa ctcatcaaca ctgtatttct
    28561 tttctccaac taacacatct tctgtagtaa aattaattat cccttcatga tttcctacaa
    28621 cttttcctaa tacagcttta accttaacct tatctatatc tctaagttta attatccaat
    28681 tttttatagc agtcttatca gcttctactg cttttggcat acatagataa ttaaattcct
    28741 tagtctctaa aaaatctaat gcagtttgaa tatctgcttc tccttctatt acataaacta
    28801 ataatttatt tggagtgtta acatttccca tcaaggctaa atttatatat tctttatttt
    28861 cagcagataa atcaactggt atatcctctt tttcatgtat ttcattaaga cctagtgcct
    28921 tagcatcttt aagcaccatt gcaattattc ctctagctga acgttcttta acagttgtag
    28981 ctagctcctt aaatgatatg ttgatacttg gtaatcctat agccatacta tcactctcct
    29041 aaatttaaat tgatttcttc catattttca tatactacat cttcttcata tacactatcc
    29101 agatagttta gtgtaagtgt aaagttcaat acataaattg actcttcttt tttaatactt
    29161 tgttctagct ttgttatatt taattttctg tctgttacct ttatatttct gttaaatagc
    29221 ttatttaatt catctgaaac ttcatacatt ctaatactct ttgactgtgg ttttggtaag
    29281 tattttatag agataacaat atctttgtta ttcgtattta acattacagg tgtacaaata
    29341 actgatagta tacttacaaa aaaacaagac ccttcagagc cttgcacact ttcatcatca
    29401 ataaaaatac tttcagtaaa gttttttttg agaatctcaa taattgtttc taatatttcc
    29461 ttgtatttaa gcaaataatc accacactat cactaaatat tcttgcttaa tctgataatc
    29521 ttactataaa tgatataagt catttttaaa tatatatctc atttattcct gtcaagtatc
    29581 ttctatattt ttaggtaata ttattctttt caatctatca cctccttcaa tatggatttc
    29641 tttcacaata acattatctc acatatttta ttgcctattg ttgcaatggt gtatataaag
    29701 tgttgtaaaa ttgtcctatt ttttataata tctcactcaa tttaaatatc atctttttct
    29761 ttaaattgta gcaagatgtt ctatctaagt gtagtttcat agaaatatat gtcatattgt
    29821 tttttgtctt actattataa aaaagattaa aaaattccat ttctatatca tttagacacg
    29881 ttagagcatt ttctatcttc tctttttcta tttctatatc ttctttttcc atttttaatt
    29941 ttcttaatct ttcctctctt tttaacacct cattttccac agaagagctt atgttatatg
    30001 tgttactagt cttttctgta tataccattg ctccacatcc agagtattca ttctcacatc
    30061 tttttatgtc caagttaata ttctttatct ttatatccaa atacttataa ttatgtaatc
    30121 ttttatctgt ttctttaaat aatatatctt ttttgttttc catagtactt cccctttttt
    30181 atatacttat tattagcctc aaatttacaa taatctcttt agtgcacagt tttttactaa
    30241 tctcatagct ttcaagaaca tattgttcta taactgtata aaaaaaactt cttctatatt
    30301 ttgttctaaa atactggaaa tcttcttagc cataagaagt ccaggatttt ttatgcctct
    30361 ttctatttga gatatataat catttgtaat tccagccttt ctggcaagtt gaatttgtgt
    30421 catacctttg cattttctat aatatttcaa gttattttta aacattgttt tatacctcca
    30481 ttactattaa gaatattttg ttcttcttca cttagattat atagaattat ttgttcttta
    30541 tcaatagatt ttagaaaaaa atattctttt atattttatt agttttattt tttagaacaa
    30601 tttgttataa tctatacata aggtggtgtt ataatgtttg ccaaaagatt aagagaactt
    30661 agaaaggaat ttggattgac tcaaagagaa cttggagaaa aagtaggcgt ttcccaaaga
    30721 gtcttaggct actatgaaac agaaaacaga tttcctgatg aacacatttt aaataaattg
    30781 gctgatgtat ttaatgtatc agttgattac cttcttggaa gaaccttagt taaggaaaat
    30841 attgatacag tagctgcaca tagaaaaaat ccacatgaag agttacctga agaagcacaa
    30901 gaacaactta atgattatat agaattttta ctaaataaat ataaaaaaaa ataaaccata
    30961 tgatgagcag tctatttttc tgctctttta atataattta aaggcaaaca tacattctat
    31021 tttaggggga tttttatgaa taatttggat aagctttttg aattagcttc tcaagaagag
    31081 ataataattc attataccac ttatattgca ggtgatttag aaggactata tataaacaaa
    31141 catggtatta aaattatatc attactcagt aatttaaaac aaaactcaaa aaaattgaca
    31201 tccatcttag ctgaagaact tggacatcat ttcactagcc ttggatacta tgtatcctct
    31261 tataacgatt attatacaaa aattattata gataagtgcg aaaataaagc cttaaaatgg
    31321 gcttgtgagt tcttaattac tgaagaagat ataataaaca taattaactc tggcatcact
    31381 tgcgtttatg aaatggcaga catacttaat gtggatatta ctttctttca aaaaagatta
    31441 gaatttttat cattaaaaaa acagtcttta caacttggaa ataataaata tttaatatta
    31501 actaatttgc cgtatttcta catatttgat ccaatttcat aataaaaata gtagtaattt
    31561 taactggctt aacagtaaaa tctatatatt tattttaaca aattttggtt tctataaatt
    31621 caatattaat atataataat gatatcatat tatttaaaat tctaaaaaca aatatatgct
    31681 tttgttaaga aaggttgttt gatgaatgta taaagaaaag ttgtttgata attattttaa
    31741 gttcttagca ctattatttt ggccaattat gtggtataag tggatagtaa tttcaaatgg
    31801 tactcttgaa aatatgttat ttaccatata tgcaatcgtc gcaattgttt tcatcatttt
    31861 atattcggtg tttatgataa aatataaaga cattacacaa atagatttct tttacaggat
    31921 aagtactcta cttgctttta tatttacact ttttagcttt ttaatatacc caaagagttt
    31981 atttttcttg tatttaaaga ttatttttac tggaatatac ctttattatt caatagtaaa
    32041 aactcttaaa tttaaagatg acgaaggtgt agtgggtatt atgagctcac ttttattaat
    32101 tgtaataact ttgttctact aaatttaatt ctatttgact taatatggct ttaaatatta
    32161 gacatctaca ttattttctg atattattta agatggtagt cttatattaa taatgaaata
    32221 cactatgaag gagttaacta ttatggaaaa aaaagtttta gcaacagttg gagaaaaaga
    32281 gataactaat attgatattg aaaatgcatt aaaatcatta gacccttatc aagctatgca
    32341 atttaaaact gaagaaggaa aaaaacactt attaaatgat ttagtgaatc aagaattatt
    32401 cttcttagat gctaaagaag aaaaattaga tgaagaagaa atatttaaat tagaaatgaa
    32461 aaagatagaa gaaaatgtat taaagcaatt tgccataaat aaagttttat ctagtgttaa
    32521 tgtaacagaa gatgaaaaag taaaattctt tgaagctaat aagagctctt tctctaaacc
    32581 agaaagtgca actgctaaac atatattagt agatagtgat gaaaaagcta aagaaatact
    32641 tgctcaaata aaatcagaag aaatatcttt tgaagatgca gctcttaaac attcttcttg
    32701 cccatctaaa gatatgggtg gtgatttagg aacattcggt agaggacaaa tggttcctga
    32761 atttgaagaa gctgtgttct ctatggctaa aggtgaagta agtgaaccag ttaaaactca
    32821 atttggatac catataataa aattagaaga ccttcaagaa agtacagagt ctacatttga
    32881 tgaagttaag gctgaagttg aaaaaagttt attatatcaa aaacaaaatg aagtttatgg
    32941 aaataagata aatgctctta atgctaagta cggaaacctt gtaaaataca acgattaatc
    33001 ataaggtcac atataaaaaa gctgtctttt cacaatattt attagaaaag acagcttttt
    33061 atattaaagt ttaaatattc agtttcatat tatcattctt ttattgtaaa gaactcagct
    33121 taaaagttta aataagattt ttaattatca ttagctaaat ttttatatcc acgtattatc
    33181 tcataacagt aacctattac atctcttctt tttattatcc caataaatgt gtcttggtca
    33241 tctataacag gaatgaaatt ttgatttaaa gattttataa ctaaatcttc aatatctgcg
    33301 ttgatggaaa caggagaatt atccattttt ctatttatat ctgtcactgg aacatcttcc
    33361 aatcccttta agtccagatt taaatcattt tttaatgtcc ataaaaaatc accttctgtt
    33421 atagttccaa catatttacc atccttactt attattggaa ttgcagagta tttgtgatat
    33481 tccatttttt ctagggcttg ccttatagtg tagtcttcat atatatatgc tacttcactt
    33541 tttggtgtta gaaagaataa tatgttcatt acactaccca ccgttactga aaacttataa
    33601 atattactca atatttataa tgttactttt ttgcagtaac tttctccttt cttattttct
    33661 aagtattctt aatactatac gctgtaacaa gcgtttttcc tttgctcatt ttaatcttat
    33721 tatgctttat taatattttt atatggtcta tcagtatcac ttattctctt tttagaaaat
    33781 ttactaaaaa ttaattgact taaacttagc cataataatt ttactctaca ctattaattt
    33841 taacatattt atctcattat taatgatttt attttaataa ttttaattta ttttgctatt
    33901 aattttattt acaaaaactg aaaaaaccgc taaatgatta gcggcttatc tctcatatta
    33961 ctagaacact aatatatatg aatttttaat tataatctaa ctacgttagc agcttgagga
    34021 cctttagctc catctactac ttcgaactca acgctttgtc cttcttctaa tgttttgtat
    34081 ccatctccag ttatagctga gaaatgtacg aaaacatctc cttcaccttc tacagatatg
    34141 aatccaaatc ctttttcgtt gttaaaccat ttaactacac cagttttcat taataaatcc
    34201 tcctaaaaaa acacaaatat ttagctactc ttttctgtaa ccttattact actctatcat
    34261 atatagctat gtttgtcaat tattattatt tttatacatt aataatcaat ttttttactt
    34321 tataatttta aatgattatt atcttttaca ctttattttt ctttataatt ttttaataat
    34381 ttatttttat tatatggaat cttatggact tatcactcca acctaaatcc catggcacca
    34441 acattctatc agtattatga caagttacca aaggataccc tcttgagtct aatcctgtca
    34501 ctgtcgaaac atgtgttatt ctgcctttct tctcataacc aacaaaatcc cctggacgta
    34561 aattataagc ttctttgtat acttcttcat aggaaccttt agcaattaat gaacctcttc
    34621 cactatacag tgcataattt ttaaaagctt gtgcatttac ccatgctttt gtacctgctc
    34681 tctcgctata attccaaatt gagttctttt tgaaccttcc actttcaaac attatctgtg
    34741 atgcaaagtt tgcacaatct ccaccttctg gattataatc cctatagttt ggattaaatt
    34801 tcataccatg ttcttcatca gctgcggctc cacagtattt atgtgcataa tctatagcct
    34861 ttttttgttc atctgtaagc tgtaactcta caccatcttg ttgaccaata taatttctta
    34921 tatcatcaga ttttatattt tctaaattta aagagtctgc aaaagggtct gtataccact
    34981 ctttagttat gacatattga ttatctataa tttttacatt tagataatga tatgtaccta
    35041 ttctaaaggc attttctttg tccttttgat tttcatatga atacttatat tcagtcgata
    35101 ctgaacagat tataccatag aggtcttttt ctttttcttt tattttttta atcttaaccc
    35161 ttgtctttat gtctttaaat tttacacctt gcttactaga ccagttttct aaatacttca
    35221 tcttttttac ttcattttca taagcccaca gaccaaattt tttatccgtc tcataaagac
    35281 cttttaaaat gttttcattt ttctctaata tcgccttatt tctgtagtca aataagtttt
    35341 caagcaatag tttatattgg tcttttacat catcaccttc aacatttgca gattccatga
    35401 ctttttcttc catatttttc atacttaaat cattgattaa aaatacacct gctgtagtag
    35461 ctaaagacaa aaatacaaat gccattatac ctttgatgta cttattctta tttttcagta
    35521 ttttcaaagc tacacctccc aaatagtaca ccttacaaat caaattacaa agaatattct
    35581 gtcacatata gtaattttca tatttaataa ttctctattt tattatgtcc aatattaaaa
    35641 attaattgta tcaatacact gtttttttta caatttttta ttttataatc tacttttttc
    35701 ttttttttat agtaatatat atgtatataa atattttggt atcattataa ttaatacata
    35761 ctaatggagg gttttttatg caaaaaaaag tcgcagcaat taatgactta tctggaatag
    35821 gaaaatgctc attatcggtt gctataccta tactttcagc attaaaggtt caatgttgtc
    35881 catttccaac ggccatactt tcaagtcaaa ctggttatcc tgaatttact ttcttagact
    35941 ttactgatga aatggtaaaa tattcaaatg tttggaaaaa tttaaaagtc aattttgata
    36001 gtatatatag tggttttttg ggttcaaagc atcaaattga gatagtggct aattttataa
    36061 acgattatcc caatgctttt atcgtagttg accctgtgat gggtgataat ggagttatgt
    36121 atccaatatt cacagaagaa atgagacagg aaattaaaga attagttaaa cattcagatt
    36181 taactactcc taacttaaca gaggcttgtt tcttgactgg aaatgattat actaaaagcg
    36241 attataatag agatgagctt atacatattg caaaatctgt atctgatttg ggacctagca
    36301 aagtcgttat tactggtata ttagaagatg ataatatatt aaatttggct tacgatagag
    36361 ataatgacca tgtattcttt acatcagtca aatataataa ttgttcttac agtggaactg
    36421 gagatatatt cacatctata ctttgtggta tgttagtaaa taaacatgat ttgggagtag
    36481 cggttaatac tgcaacagat tttatatata aaactataaa ttacacatct caatttgata
    36541 ctgatagaaa tgatggagtt atgtttgaaa actttttatc agatttaact aatatataaa
    36601 atcaaaaaaa ctatccattt tataaatacc tataaatcat aaaatttagg tatttacaaa
    36661 atggatatta tttactaaac tattggtatt tcaattgtta ccatagcacc accagttttc
    36721 acagaatttt caatacataa agtcccatta tgtttttgta caatattatt tacaatatag
    36781 agtcccattc cataatgaga tttagaattt ctactcaaat ctcctgtata aaactgttct
    36841 gtagcttttg acaaatctgc tttagaaaaa ccacttccac aatctgtaac tacaaatttt
    36901 aagtatttat tacatactga tacacttatg tatagtttac tctggcttgg tgaataatct
    36961 atagcatttg aaattatatt cataatagct ctgaataaca gttctctatc tatagttata
    37021 tttttaggca aattatcttg tttataaaac tccacatcta agtttttagt actagctaat
    37081 gcttctattt gcactaagac atcttttata aactcactca tactgattgc ttctttattt
    37141 aatatatagc cattctctgt ttttgatata tctattaaag tattaatata tttttctatt
    37201 tgagtgacac cctttgagat atattccatg tattctaata actcaggatt ttcatttatt
    37261 tcaattaata agtcagtatt tccatgtata attgttagtg gtgtttttat atcatgtgcc
    37321 agtgcagata tctgttcctt tctattttct tctagcatcc attgtttttc taaagaatct
    37381 tttaacgcca acttcatttt atccatagaa aaaaggacat tattaatttc ataaatttta
    37441 ctagattcta caacaaaatc taaatcctgt tgttctattt tttctgtagt attttttaaa
    37501 agctccattt caatattaaa tttcttgcca aatacttttg ataatataac aatttcaata
    37561 aaaaatatta tactaaaaat aatcataccc aatgtctctg gttttggtaa atatgctctt
    37621 aaaattggag aactatactc tgatactatt ttgtatttta ttataaaaac ttcatctttc
    37681 ttaaaaaact ttatataact ttctgaatat gcatgccttt cttcaatatc tctcattaaa
    37741 ttccatactt cttttgattc ttttttatta aaggtacctg aaattaaatt cccatctaaa
    37801 gtataaaccc catactcata tatacttgga attatatctt cagttacttt ttcacttgaa
    37861 ataatctgtt ctttatattt attgaattgt ttttctgcat aattagctgg taaaattaca
    37921 cctgatgata ataatacaga aaatgataac attaaaagga gtacaattga aattgtcata
    37981 ataaagaata aagataaata ttttataaaa atagttctta acttttcaac ttttttgttg
    38041 ttagattttt tacctccaag catttgtacc ctgtcctcca atagtttaat taattcataa
    38101 ttaataccta gccagttttt tatttgatta ttttatatct ttttattgct aagttatatt
    38161 ttaacataat aatataaata aacagtcaat ttttactcta ttcttctgat tttttacctt
    38221 cccatttaga aaaccaaata cataaaatta caaatgctaa aattgttaca aaaccacaaa
    38281 ttaaacctgt tgattgatat gcttttattg attcaacctt tatatattca accgttttag
    38341 aacttgaaaa agacgaccaa atagttataa atctaacacc ccatgcacaa ggaaaatact
    38401 gccaaatacc agaacccaat ccagttaata ataatgctga aagtaacatt tcaactatac
    38461 ctactccaat tgatgctcct tttccgaacc ttaaacttaa gaataaatgt aatatgtatt
    38521 caaataaatt acacccaatt aaaattagaa ttgaaatcat ataaaagctt gatgtaaagt
    38581 aagccttaga tataccaaat ttaaaaccta atacagacac taatgttgct acaaaaccaa
    38641 gacatactaa gattatatat ttacttataa gagtaagata ttttggatta gatgttgtta
    38701 acatatgttt ataattccct gcataatatt cttgttcaac aaacattgaa cacacaacac
    38761 ctgacaataa aggaaaagct attgctataa cttgtaaata tcctactatc tttagtatat
    38821 tatcagctcc tgaaacctta taataccata aaaacaatcc catccccatt aaagcaatgc
    38881 aaatgtgcat tagtattata aaatttctct ttaattttat taaatcagaa caaaatattc
    38941 ttattaactg tctcatatta cttcacctct ctattattga accaaaccct tgtagctaaa
    39001 gcaaataata caaataaaac aatagatatt aatactcctg gcaaaataac agcattattt
    39061 aaaaattcac ttccctctgg tacaggaaga ccatttggca gaatatgcaa tacaacagcc
    39121 attaatcttg aaggaatcga atatggaaac atataccaca tttctgtagt tgagaaaaca
    39181 gcaactccaa caatagtgaa gaacatattt attagaactg taacaaatat accaaatttt
    39241 gaagctaaaa acatacaaag aggaatctgc cacataaata gtaaaataat taatatagtt
    39301 gcaatgatat tttgacctat tgaaatagtt tctttataga ttatcccaac aaaaactaca
    39361 cctattgcag aaataattga tgctataaaa caacaaataa tacagtaaat catttttcca
    39421 gtccaaacgt ctttcatttc aattggcaag ggtaaaatac cacgatattt acttttttca
    39481 tcttttaata ctattaaaga agatattaat gttaatgctc ctggaagaaa tgtcgtatac
    39541 caccagttga aagcagcatt ttgccctcca cctagtccac acaaaagtat acaaaacact
    39601 ggaactatcc atatcaactt cattccaaaa gttcctttca tcttttgaaa ttccactcta
    39661 ataaatcttt ccatattact cccctctctt tctattttca gctacaactt tcataaaaag
    39721 tttttctaag ttttgttctt ttgatacttc tccttgataa cccattactc cattagaaat
    39781 tattcctatt tcatctacaa cttgttcaac ttcagacaag atatggcttg ataaaataac
    39841 tgtaattcct tgttttggaa aagaacgaat caattctcta agttcttcaa taccaatagg
    39901 gtcaagacca tttgtaggct catctaagat taaaagtttt gggtgattta ataatgcaat
    39961 agcaatacca aggcgttgct tcatgcctag agagaattga cctgaacgct ttttaaaggt
    40021 atctcttaaa tctacagtct ctaaaacttc ttcaatgcga gagtcaggaa gaccaagtaa
    40081 agtagtatga acttttaaat tttctcgtgc tgttaaattc tcatatatag caggagactc
    40141 aataagagaa ccaatatttg ataaatcctt tctagtccat ctatgtccct caaacattat
    40201 ttttcctgaa gttggattta aaatcccagt tatcattttt aataaagtag atttcccagc
    40261 tccatttggt cctaataatc catatataga attagattta attgctagtg aaatgttatc
    40321 aactgccatc tgctccttaa attttttaga aactcctttt acctgcaaaa tataattact
    40381 cataattttc atcctttctt ttgtcaaact tatttacaag aatcattcta aatcataaat
    40441 ataaggattt tataagaatt aaaaaacaaa aaaagatgtg ttaaaaacac atcttttttt
    40501 atcttaattc tattaatttt ccattgtagt atatctctac ttcttcaatc caaatgctat
    40561 tatcttcttc ttggatgaca tttttaataa tattatttat agtatatatt atattttgtt
    40621 cattcaaata atcatctttc caagaaactc tacttttttt atctggttct atcaagttta
    40681 ttttaacaag attatctttt tccttaactt ctacttccat gtctaaacca ttaaaatact
    40741 catttgataa tgtatctaag attaatcgca gtttatcctt aagtgaactt tcttcatcaa
    40801 tctgtaagta ctttactatt ttctcatcct ttgaaattat ctctataggt attttagtag
    40861 tttttttaga attaatcatt ttactagttg tttcttcttt tgcatcttca tccttcttta
    40921 caactgctga ttctttagaa tcaactagct caactgcact gatgctataa tccatacatg
    40981 ccgtactcaa taaagtagta gcaattatta aaaaacttac tgctatttta ttcataagta
    41041 tccctctcct aattctcttt cgatacttat tataccatag tatagattat atttttatac
    41101 ttttaaatca atttataaat tattatagta aattatttac aattctaagc tatttgaatt
    41161 tatttttata gtatattctt tctctaaatt cgacagattt ttattcaaaa gcccttttat
    41221 ttccctcgta tttctatact tgttgctatg ttcttcatca tgttcatagt catatgaatc
    41281 attgtcatat tcttcatcat tattaaattc ttcaattgct tcttttgact ttatatcaca
    41341 attaaattta tacttattta ctggtatatc aagtcttacc ttagaatttg gaacttctat
    41401 gtccaggaac ttagcaattg gcatatcaga gtacaagtaa aattcacttt cataatttga
    41461 atttctacct attttctctt ttatatttac attttcaggt atatactctt ctatattatt
    41521 gataaataca tcttcattct ctgaagatat atacaatgac tctgattcta tgtttacatt
    41581 tttaacgcct aaaattgatt ttgttgacaa gtgaagattg ttattagata ttaagtttag
    41641 gttttctaat ctatttactt tgttctctgt tatagctcta ctaaaacctc cataagaagt
    41701 tttatatgtt atagtatttg aagctacatt atcgtataca aataagtgtg agcttatact
    41761 tgatgcattt atgtctacat tatttggtac atatattata atttctctat aatcatgtga
    41821 aaacatacta tttatagatg tatttaataa ttggtcgaaa tttttaatct ttttaatttt
    41881 attttcatat tttctttcac cttttacaac taaagtctta tcttttaaag ttacattgta
    41941 tttgtaaaac tcattatttc ctcttgttgt tacacgcaca tcttcacttg aacttttctt
    42001 tattgtaacg tttatattgt caacagctat atctaacttg tcaatattta ctttttcttg
    42061 ataaagttta ttttcttttt taaattcagc ttccttttct aaaccaaagt ttattaggct
    42121 tggcataact aaaatccctg accatgttgt ccctattata cctattacca ataaaactgc
    42181 agcaaatata gcaagttttt tattcatgtt tatcttctcc tttcttattt tctatatctt
    42241 caaggtcctt atatatcgcc tcaaaattta tactatcatc attatattca tcagtttctt
    42301 ttatgctttc ttcttctcta ttgtagtaat cattattata tgaatcttca ttaatgtctt
    42361 tattcaaatc ttcattaatg tctttattca aatcttcatc atttaaagta gctttttttc
    42421 ttttttccat cttttcattt gcctttatat aaatattttt tctctttatc caatgtatat
    42481 aattatttag taattgttta cctagtttta tggcaaataa taaaatttgc catgcaagta
    42541 tctcaagccc tacaaatgct atggttgcga aaatacaaaa catcataaca tctggaacca
    42601 ttgctataaa tttaatcaca aatggaatta atataacaaa agcaattaat gagcatacta
    42661 atgcaatagt agcagataca atagcacata caaacgcaaa tgctggaaca attagaaata
    42721 aagataacac tactaataac ccctttataa cttgtctgga taatcctcct acgccttctt
    42781 ctatacttgg tgtaagttta tcatttatat tttcttttga tttattgtac ttttccttag
    42841 cttcatcttt caagatagag taatcctttt ttacatttga tttaaatcta ctaaatttac
    42901 catttaatat gtctgtattc attatttttc catctatatt ttcttttgat ttactatact
    42961 tttcttttgc ttcatctttt aagatagagt aacctttttt catatctgat ttaaatttac
    43021 taaacttatc attcaaaaca tctgtattta ttattttttc ctcttcaata tttttttctt
    43081 tagtttgtaa gataagttct tttgctatag attttggtga gccaagtgat gctattatct
    43141 ccatatcact ctttccctct ataagaccat ctacgaaata ctcttcataa tctctcaata
    43201 tatcattaac ttcgctctca gaaaaatctt tttttagata atcctttaaa atttcaagaa
    43261 actcagtttt attcaagttc attacctccc tcatcttcac ttattaaaat ctcaacagcc
    43321 tttacaaatt ctctccactc ttccatcaga ttggataaat tctcttttcc tagtgaagtt
    43381 attctatagt attttctagc aggaccttca ttagattcta gtatatatgt ttcgaaatac
    43441 aaatcttttg taagtcttct tagaatagga tatattgttc cttcatttac atctattact
    43501 ttagagatgt tatgtactat ctcatatcca tacatatctt tcttagatat gagagcaagt
    43561 acacaaattt ctaaaacacc ctttctaaat tgtgtgttca tatactccat cctttcttta
    43621 aatacacagt attgtgtttt acaaggtact atgttaaaac ttcttatatc ctcataataa
    43681 cacagtacta tgttctgcac aatagttatt tttaaatatt cttgttgatt aaatccttct
    43741 ataaatgtat aatactctaa tcagatatat tttatgaaag tggtgataaa ttggctaaaa
    43801 tgaaagatga taaaacaata ttaatcctaa ctgctcaatt tggtgctgga catataagtg
    43861 ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag taactataat gttgttatac
    43921 aaaactttat aaatgcaagt attcccatga tgaataagcc tatggtaaaa ctttatgaaa
    43981 acaatacaaa atatactcct ggattatata attactatta ttactttaag aaatcgtttg
    44041 attcaagaca tgatttatct cataaattat atacacctaa actttctgag tatattgctg
    44101 atataaatcc agatttgatt atctctacat ttccactagc tgctgcttgt gtaaataact
    44161 ttaagataaa aaatcctgat ataaatatac ctacacttac agttataaca gatgttgtag
    44221 atagtatgga atgggttttt gaaaatacag atttgtattt tgttccttct cctgaaataa
    44281 aaaatagatt ttttcagaaa ggaataaatc ctgactccat aaaggttact ggagttcctg
    44341 ttgataagag atttcaaatt gaaagtaaag aactatgttg tgataaatat agactattac
    44401 ttctaggtgg aggaagaggt ctatttgata tagatgagga ttttatgcat tggatagatg
    44461 aatttattga agaacatagt aattctatag aaattacaat agttactggt aaaaacaaaa
    44521 aattatatga caatctaact cataaaaaac ctctaaaaaa tattaaagta cttggatttg
    44581 taaacgacat gtataatcta ataagagaat gtgacctaat gctaactaag cctggaggag
    44641 caactatatt tgaagctatt caatcacaaa ctccagtact cgtaaaaatg ccaaaagttg
    44701 gacaagaaat tgaaaacgct aaatttataa tagataaggg tcttggcatg atttatagtg
    44761 atgacctaga ccttaaaaac atattttata aattagtatc aaatgaattt gattctataa
    44821 taaactttat gaaaaaaaat ttaaaagaat ttaaaacagt cattcatcca gagaaaattg
    44881 ctgattatat atcagaatta atagataatc attacagtta aattgtacta ttaaaaaatt
    44941 tcaaacataa aattaaaggc aatcaagtat gcttttacac ttgagaattt actcatagtg
    45001 tttaataata cttgattgcc tttatcatat caaaatcaaa ttgttatatt agtatatatt
    45061 tttcttgtac tgcatataca gttgttgtat ttcttccata cttctcttta attctatttg
    45121 taaatctgaa gcagtctggt aatccttatt ttctatagct tctctcactc tagtgaatag
    45181 tgattctcta gttaaagtat ccttcataat attgaacttt aaatccgata ttgagttcca
    45241 aactacctca tcaagcgcat ccatattttc ttttgtgtgt atcttgacaa tacttcttat
    45301 tttttgaatc ccagcctcta ttattctagt ttttaatttc ttttgccact tatctatagc
    45361 tccaatctta aatgattcta ttatactatc agtaaataca tcaccttgtt ttaaagattt
    45421 taattttgaa gcatatattt ctaagttttt catattttca taaactgtag ctggtggtat
    45481 tccaaatctt gcatttcttt cttccaaact ataatgttca aatacatcat tttcatctct
    45541 atatgctcta tctttttcta aatagaaact ttcttcacct acatttttag ataactcttt
    45601 ttcaagttct tttgtactaa gaccactctt agctgctgct tttataccat ccaacattgt
    45661 ttgataacat cctgctatta ctaaatacgt gttagataga gggcttggtg accttaattc
    45721 aaatcttaca gtctttggat ttttcatatc tcttattaat ccaacaagca ctgacctatt
    45781 tcttgatgga atctcataac tatgccctaa agatgtaact atgcatacag gagcttcaaa
    45841 tccaggaact aatctattaa atccatcatt agaagctgtt acaattgggt ttaatacttc
    45901 ataattgtaa agaagtccca taagagcccc atatcctagt tcacttagat aatcttcttt
    45961 taaatctttt ggagcaaata gattttttat acttccatcc tttaacttcg cacttactcc
    46021 tacatgtgta tgtccaccac ttcctgctac tccatgaatt ggttttgctt taaatgtaac
    46081 ttctagtcca tgtgatgtaa atacatcctc tataacatct cttactaata attcattgtc
    46141 tgctgcttgt aatggtgtag aaaacttcca agaaacctct aattgctcca tagcatggtt
    46201 tgttcttcca tctatactaa tagaactttg tattcctccg acctctttgt gagccatttc
    46261 agggtttaca cctagctttt gaagtataat taaacttttc tctaaacaag ttcttataat
    46321 accatgagtt cttttccagt attgctcttt taggctttgt gatacatata atttctctaa
    46381 gtcagcttta tcctcaggag tattaaccca gaactcaagt tctgtagctg ctgttaacat
    46441 tatttcctct atatcatcta ctgaatctat tcctatgtta tttattacat gtggatattc
    46501 tctaaatatt tcatacattg actttttaaa gtacttatct gctttttgaa gtactcctct
    46561 agaacatact tttttattat catgtattaa aaatgcaggt atctttaatg ttccaacagg
    46621 tagacctact tcttcgtcta tatattccat attgtaatct acataccaat gacaactttt
    46681 atctggcatt aaatctactt tcgcattatt taatgtagct atattataaa gctcaacact
    46741 tgaaccatca gtctgtatag ctgattccaa aaatttatct atatcctcca aaaataattc
    46801 tacaggaatc ttttcatctg ttgcatttcc tcctaaatca acccccatta aggaaacaaa
    46861 tcttatattt ttattgcttt ttaggatttc tcttaattct tcattagtgt gtttatcttt
    46921 ctctattacg tataatagac tatccatttt tccactctcc cataattttt atgtatattt
    46981 tattttgtta aatacaagat aaatttcatt ttccttacat ttatgaattt ttcttgcaaa
    47041 ttattcgtgt tt
//
LOCUS       DAKBNN010000007        46370 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000007 DAKBNN010000000
VERSION     DAKBNN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.007"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(352..810)
                     /locus_tag="OUB02_000400"
     CDS             complement(352..810)
                     /locus_tag="OUB02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8884214.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            complement(1025..1669)
                     /locus_tag="OUB02_000401"
     CDS             complement(1025..1669)
                     /locus_tag="OUB02_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8884215.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            complement(1673..2218)
                     /locus_tag="OUB02_000402"
     CDS             complement(1673..2218)
                     /locus_tag="OUB02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884216.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            complement(2361..2804)
                     /locus_tag="OUB02_000403"
     CDS             complement(2361..2804)
                     /locus_tag="OUB02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HCT8884217.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            complement(2913..3170)
                     /locus_tag="OUB02_000404"
     CDS             complement(2913..3170)
                     /locus_tag="OUB02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884218.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            complement(3190..3675)
                     /locus_tag="OUB02_000405"
     CDS             complement(3190..3675)
                     /locus_tag="OUB02_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884219.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKEAIDTGMTKDIIAEG
                     LDVCQTILTMFQILELEDYIAEGIEIHNKKLNGRGWDLKEIGDGILNLIKNSMINELI
                     NMNNKLKCVDEKEFNSKIQFLDVNKLWDLCVPLVDLSAMKGINENIDEMKMGEFLKNI
                     N"
     gene            complement(3730..4371)
                     /locus_tag="OUB02_000406"
     CDS             complement(3730..4371)
                     /locus_tag="OUB02_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCT8884220.1"
                     /translation="MNKRIVCQDTNEIVYNYKEYQKTKHWESLKNEFYFSNECKCVLC
                     DSVKKVSLHHKTYRRIGAEELDDLIPLCNSCHYKVHRNNKQYLFNDATEKQIRYINDL
                     LEVYVDIVEELFMNEYIYLYVAMGIGFYNKNLMLFEANHIINDLKFVSAMLYEFYATK
                     LEIKLPGYFNGFDYEEIKDSLYKKMQRLSKYIYVPKKQEIELLFKKRKKEHYE"
     gene            complement(4372..4632)
                     /locus_tag="OUB02_000407"
     CDS             complement(4372..4632)
                     /locus_tag="OUB02_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884221.1"
                     /translation="MKCKVYHAIKNMKDANELIDRFETENIFFELNDIDNENVLNGEM
                     DLTDIEDIASLQFILDGDLFIFNSCDEMIFMNKYTQKELGLQ"
     gene            complement(4657..5464)
                     /locus_tag="OUB02_000408"
                     /pseudo
     CDS             complement(4657..5464)
                     /locus_tag="OUB02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5476..6012)
                     /locus_tag="OUB02_000409"
     CDS             complement(5476..6012)
                     /locus_tag="OUB02_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HCT8884222.1"
                     /translation="MKKTLIIVGVVISILIMLACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLEANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            complement(6012..6338)
                     /locus_tag="OUB02_000410"
     CDS             complement(6012..6338)
                     /locus_tag="OUB02_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884223.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFGSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            complement(6341..6499)
                     /locus_tag="OUB02_000411"
     CDS             complement(6341..6499)
                     /locus_tag="OUB02_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884224.1"
                     /translation="MKTKIIGGRENECPICNGNIFKIETLIGIVWQCKDCGCMYQDMS
                     SKESKRGY"
     gene            complement(6528..7112)
                     /locus_tag="OUB02_000412"
     CDS             complement(6528..7112)
                     /locus_tag="OUB02_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT8884225.1"
                     /translation="MMKIFHFKLDGDYMRSLEEKNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFLYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQASIYRRLKKLKTEINILGK"
     gene            complement(7090..7467)
                     /gene="ssb"
                     /locus_tag="OUB02_000413"
     CDS             complement(7090..7467)
                     /gene="ssb"
                     /locus_tag="OUB02_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT8884226.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYAKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEKEIGFQVLDDEDIPF"
     gene            complement(7464..7646)
                     /locus_tag="OUB02_000414"
     CDS             complement(7464..7646)
                     /locus_tag="OUB02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884227.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            complement(7703..8407)
                     /locus_tag="OUB02_000415"
     CDS             complement(7703..8407)
                     /locus_tag="OUB02_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884228.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIRSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            complement(8459..8845)
                     /locus_tag="OUB02_000416"
     CDS             complement(8459..8845)
                     /locus_tag="OUB02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884229.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKSTIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSASVIDFRNASSK"
     gene            complement(9218..10549)
                     /locus_tag="OUB02_000417"
     CDS             complement(9218..10549)
                     /locus_tag="OUB02_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8884230.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKRIIDSNKPLDLLLLKNELEKIDRLVDVGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIVDINDLKALVTTSSGINDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMAMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYASELISDWIKDKFFLYDEDA
                     ISDEVNLLNTIEYLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVIDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            complement(10571..10888)
                     /locus_tag="OUB02_000418"
     CDS             complement(10571..10888)
                     /locus_tag="OUB02_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884231.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            11026..11214
                     /locus_tag="OUB02_000419"
     CDS             11026..11214
                     /locus_tag="OUB02_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884232.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            complement(11207..11467)
                     /locus_tag="OUB02_000420"
     CDS             complement(11207..11467)
                     /locus_tag="OUB02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884233.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNREAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            11630..11842
                     /locus_tag="OUB02_000421"
     CDS             11630..11842
                     /locus_tag="OUB02_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884234.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFAGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            complement(11861..12079)
                     /locus_tag="OUB02_000422"
     CDS             complement(11861..12079)
                     /locus_tag="OUB02_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884235.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKGYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(12105..12290)
                     /locus_tag="OUB02_000423"
     CDS             complement(12105..12290)
                     /locus_tag="OUB02_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884236.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIKFDIKSDI"
     gene            complement(12319..12705)
                     /locus_tag="OUB02_000424"
                     /pseudo
     CDS             complement(12319..12705)
                     /locus_tag="OUB02_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(12889..13209)
                     /locus_tag="OUB02_000425"
                     /pseudo
     CDS             complement(12889..13209)
                     /locus_tag="OUB02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425622.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(13238..13489)
                     /locus_tag="OUB02_000426"
     CDS             complement(13238..13489)
                     /locus_tag="OUB02_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884237.1"
                     /translation="MKIIYKIKGVKRLKISDFNRNHIGKAVQLQEIRFNTITDCIITE
                     VEVGEIVVMFYMQENDDTGYKAITYEDLENGDCKLKLLN"
     gene            complement(13467..13610)
                     /locus_tag="OUB02_000427"
     CDS             complement(13467..13610)
                     /locus_tag="OUB02_000427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884238.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            13758..14144
                     /locus_tag="OUB02_000428"
     CDS             13758..14144
                     /locus_tag="OUB02_000428"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884239.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            complement(14749..15267)
                     /locus_tag="OUB02_000429"
     CDS             complement(14749..15267)
                     /locus_tag="OUB02_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8884240.1"
                     /translation="MSKMIQCKSCSKEIASNAKSCPNCGAKNKKPFYKRGWFIVIVVL
                     VLLYAIGTSGSGGTDDTAKGNDDGTNKTASVDKPEEPKKEKYEIVGDITSETDSMATY
                     IKGVIKNNSGHDISYAQVTFTLYDKDGNQLGTAMDNINDLKKDGTWKFKAMGMNTDGE
                     IASYELSEITGF"
     gene            15643..16026
                     /locus_tag="OUB02_000430"
     CDS             15643..16026
                     /locus_tag="OUB02_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8884241.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHRNSFESFISAL
                     HDDEEISANKLKSLEKFIEDWEEDK"
     gene            16748..17935
                     /locus_tag="OUB02_000431"
     CDS             16748..17935
                     /locus_tag="OUB02_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCT8884242.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIALERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            17955..18755
                     /locus_tag="OUB02_000432"
     CDS             17955..18755
                     /locus_tag="OUB02_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT8884243.1"
                     /translation="MYYVYKYIDPTTEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLESFGSCSLISVYPRWKEYTATNKKILIDITLEIRIGSL
                     ANLMELMPTFEYKIAEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            18775..19509
                     /locus_tag="OUB02_000433"
     CDS             18775..19509
                     /locus_tag="OUB02_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT8884244.1"
                     /translation="MYYVYKYIDPATEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLDPKFNVTGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISNNEEIFIGNENIKIKYSFETTLEGIDLEP
                     CILSVAYKKLKESTVGTLISVKREYMPENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSTNS"
     gene            19629..19781
                     /locus_tag="OUB02_000434"
     CDS             19629..19781
                     /locus_tag="OUB02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884245.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            20170..20472
                     /locus_tag="OUB02_000435"
     CDS             20170..20472
                     /locus_tag="OUB02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884246.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            20508..20966
                     /locus_tag="OUB02_000436"
     CDS             20508..20966
                     /locus_tag="OUB02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884247.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLHWRVTSTLGNILGFEGNIDVYKAGKIVSSQFVS
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            21216..21419
                     /locus_tag="OUB02_000437"
     CDS             21216..21419
                     /locus_tag="OUB02_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884248.1"
                     /translation="MSRIVLTFKNNNKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            21438..22343
                     /locus_tag="OUB02_000438"
     CDS             21438..22343
                     /locus_tag="OUB02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HCT8884249.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFAKGNETTKRDITIRSVQVFPEGVTGYLYYAKDIVDQVAGRDVVLVDIG
                     GKTTDIALVQGNKATNPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(22594..22770)
                     /locus_tag="OUB02_000439"
                     /pseudo
     CDS             complement(22594..22770)
                     /locus_tag="OUB02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            23013..23225
                     /locus_tag="OUB02_000440"
     CDS             23013..23225
                     /locus_tag="OUB02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884250.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            complement(23268..24107)
                     /locus_tag="OUB02_000441"
     CDS             complement(23268..24107)
                     /locus_tag="OUB02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8884251.1"
                     /translation="MKICITVGHSILKSGACTSANGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKIYKIPRVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMHKHTIVYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKISPMTKENYTMIKGNDRFETLYKALDF
                     IDR"
     gene            complement(24107..24538)
                     /locus_tag="OUB02_000442"
     CDS             complement(24107..24538)
                     /locus_tag="OUB02_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCT8884252.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACVFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            complement(24606..24776)
                     /locus_tag="OUB02_000443"
     CDS             complement(24606..24776)
                     /locus_tag="OUB02_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884253.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTV"
     gene            complement(24788..25135)
                     /locus_tag="OUB02_000444"
     CDS             complement(24788..25135)
                     /locus_tag="OUB02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884254.1"
                     /translation="MDRGNRVIYNQDGKILLQTGETTGDVLEHDTITELHYLDVEYGS
                     IDYSKQYIESINPVTKELNIKNIEIVLTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENMKLNNNI"
     gene            complement(25498..26802)
                     /locus_tag="OUB02_000445"
                     /pseudo
     CDS             complement(25498..26802)
                     /locus_tag="OUB02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26820..27599)
                     /locus_tag="OUB02_000446"
     CDS             complement(26820..27599)
                     /locus_tag="OUB02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT8884255.1"
                     /translation="MAEQQYFTLVTEIGKAAIANASITGEKVDFAKMKVGDGGGSSYT
                     PTESQTALKNVVWESTLEHAQADKDNPNWVVIQKFIPGDVGGFEVREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQDLDAKI
                     DTTKTELTSNIETAKTELNGKIGDTTQLTTTDKTNIVSALNEVKASVDSIETTAEKTS
                     YNNATSNLTATNVQGAIDEVVRKIEKFNEVNISIQNDMLPI"
     gene            complement(27616..28224)
                     /locus_tag="OUB02_000447"
     CDS             complement(27616..28224)
                     /locus_tag="OUB02_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCT8884256.1"
                     /translation="MKLIDYLPSFANNKIDIQIQEALENELLTLIDEKDDLLDQFFID
                     TATWGLDDWESLLGIKINYKLDFDTRRSNIRAKLRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNGLYTGAT
                     ISTGETVTVYPYVPRDLESFGEIAICSGNDRTLEKVTLYPKK"
     gene            complement(28226..29278)
                     /locus_tag="OUB02_000448"
     CDS             complement(28226..29278)
                     /locus_tag="OUB02_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCT8884257.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTKIPNGTII
                     FHSDLPFVIIKDITIDENSKLNVSPIQALEVGIKYNLSANTEFKLIEEITGVTKIYNE
                     FDLKGGTEIETDEELKERFYKTQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIIYIKVMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKVPAVSSVAFDVEVS"
     gene            complement(29271..29705)
                     /locus_tag="OUB02_000449"
     CDS             complement(29271..29705)
                     /locus_tag="OUB02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HCT8884258.1"
                     /translation="MTNTIFPFMGVPEDYISPDNEELPIFKEFAWDFDKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWDYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNVVNTEFKDDNLSISLNVKTIYGEEEITFV"
     gene            complement(29698..30030)
                     /locus_tag="OUB02_000450"
     CDS             complement(29698..30030)
                     /locus_tag="OUB02_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HCT8884259.1"
                     /translation="MADPINEFIGIMRQEGKFHNEPSFFIGKVKDKLPDLKIEINNLL
                     LEKEDILIDSWILDRQIESFNTEINKEHKHNIKNPFIDTFEAGDMVIMFKINDKFAVI
                     SKLVVLND"
     gene            complement(30045..31562)
                     /locus_tag="OUB02_000451"
     CDS             complement(30045..31562)
                     /locus_tag="OUB02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT8884260.1"
                     /translation="MIIDRSLDSSNNMIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFLENKLPLGNLPKTGVKYTKMFIGVTGYDTIMSVYTEASKSTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIKNVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGTYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTERA
                     KTVIAEARKHLGKPYKWGGNGPSNFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            complement(31802..32437)
                     /locus_tag="OUB02_000452"
     CDS             complement(31802..32437)
                     /locus_tag="OUB02_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT8884261.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILNVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDVYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDKIKNPSVIKNGWVLIIP"
     gene            complement(32495..32845)
                     /locus_tag="OUB02_000453"
     CDS             complement(32495..32845)
                     /locus_tag="OUB02_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884262.1"
                     /translation="MNKKIPALAISIVLSLSLVGCSTNDSKNGDSKPKQNTEEIKNHK
                     IGEVVDLDGAKVSVKNVRKLESDSNVYAVEIEITNTGDTLKGEIAFELTKDTKPKTIE
                     IAYENVGKYTVFDL"
     gene            complement(32892..36284)
                     /locus_tag="OUB02_000454"
     CDS             complement(32892..36284)
                     /locus_tag="OUB02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCT8884263.1"
                     /translation="MSDGTRVLQAIIRMRDEASATLRRVAANTRSLHDSTSNASKALN
                     NVQKNLKNVGSTALKTGSAIAAVSGGILALSAKSAIEFESAFAGVKKTVNASDADINT
                     FKKNIMDLSKNMPQTAVEISAVGEAAGQLGIKVGKNASNITNFTKTMVMLGDATNMSS
                     DEAATALARFANIVQMPQKNFDRLGATIVQLGNNLATTESEIVEMALRLAGSGKQVGM
                     SEAQIMSFAGALSSVGIKAEAGGSAFSKVMQQMQLSCEMGGSKLNNFAKVAGMSASKF
                     KESFQKDAAGAIISFVKGLGDAKKHGNSAIKMLNKMGITEIGMTDALLRASGAGDLFN
                     NAIQLGNDAWKENVALTNEANQRYSTTESQLKMMKNQITQVAIIIGNNLLPFIRSAVQ
                     VVSNMANAFSNLPPAFQSTVTGILLAVTTFGLLLATVGIVSLVISKAAGLFAFFIPII
                     LRTKVAFRAFVIGMKPLSAVFLAVMGPMGWTIAAVIALSVAFTVAYAKSEVFRNKVNS
                     LVKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIG
                     VIGFHIAKLVVVLSTLATAFKAVVDTITNVLNGLASILNGIFDLIIGIVTGDSKKMMN
                     GVKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLE
                     NPIEATAKISAKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMT
                     DSISQGVESGKSKFTELQNKILNDVKPALKVLEDSGVNFKNKFKESITSIKDKFNELK
                     DILAGNIKTNIDNLVKAFEPLKPHLDNLKECFDKVKKAITDFFTPAKQVGDTVNKVSQ
                     PMDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGG
                     IILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDS
                     GVGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKEFFHNPIKGMIE
                     IVKSGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNS
                     SNGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKVGGVL"
     gene            complement(36464..36880)
                     /locus_tag="OUB02_000455"
     CDS             complement(36464..36880)
                     /locus_tag="OUB02_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8884264.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSTFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCPIFSNMELVKKFSAITPKELVKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            complement(36896..37336)
                     /locus_tag="OUB02_000456"
     CDS             complement(36896..37336)
                     /locus_tag="OUB02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCT8884265.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            complement(37352..38407)
                     /locus_tag="OUB02_000457"
     CDS             complement(37352..38407)
                     /locus_tag="OUB02_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCT8884266.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSPTNLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            complement(38408..38857)
                     /locus_tag="OUB02_000458"
     CDS             complement(38408..38857)
                     /locus_tag="OUB02_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884267.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            complement(38866..39222)
                     /locus_tag="OUB02_000459"
     CDS             complement(38866..39222)
                     /locus_tag="OUB02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCT8884268.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            complement(39222..39569)
                     /locus_tag="OUB02_000460"
     CDS             complement(39222..39569)
                     /locus_tag="OUB02_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884269.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            complement(39581..39961)
                     /locus_tag="OUB02_000461"
     CDS             complement(39581..39961)
                     /locus_tag="OUB02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCT8884270.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            complement(39945..40217)
                     /locus_tag="OUB02_000462"
     CDS             complement(39945..40217)
                     /locus_tag="OUB02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884271.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            complement(40231..41154)
                     /locus_tag="OUB02_000463"
     CDS             complement(40231..41154)
                     /locus_tag="OUB02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884272.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            complement(41183..41785)
                     /locus_tag="OUB02_000464"
     CDS             complement(41183..41785)
                     /locus_tag="OUB02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCT8884273.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            complement(41811..42137)
                     /locus_tag="OUB02_000465"
     CDS             complement(41811..42137)
                     /locus_tag="OUB02_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884274.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            complement(42196..42957)
                     /locus_tag="OUB02_000466"
     CDS             complement(42196..42957)
                     /locus_tag="OUB02_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCT8884275.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            complement(42935..44212)
                     /locus_tag="OUB02_000467"
     CDS             complement(42935..44212)
                     /locus_tag="OUB02_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8884276.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            complement(44225..44428)
                     /locus_tag="OUB02_000468"
     CDS             complement(44225..44428)
                     /locus_tag="OUB02_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884277.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            complement(44429..46168)
                     /locus_tag="OUB02_000469"
     CDS             complement(44429..46168)
                     /locus_tag="OUB02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884278.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLNKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
BASE COUNT        14759 a         7771 c         5431 g        18409 t
ORIGIN      
        1 aaaaaaaata aatatattaa agtattttga attattttct tgcaagaaat atttttttga
       61 ttagaattat taattatctt tcaactattc cttaaatatg tattttcggc atagttaaaa
      121 agaacctagc atttccaata ggttcacata tcctataata atccacatca cttcactgta
      181 tgaaacttta attattaagt ctcactaaat tttattaaat tagctataac taaagttaaa
      241 tacaatatat gatttaatta ttaaagtaaa tatatatata atatatgttt tgctttaatt
      301 gatttcaaac ataattaaaa catattcttg caggatttat ttcatatcta attacttctt
      361 gcttgttctt attcttatct tagctatagc tgcttctatc tctgttgcat ccatacccat
      421 gccaacacta ttatcctctg cttgtataac tgtacttgta ggagttccat atatacgatt
      481 tataagatac tgattggctg ccaacataac cctcttatcg ctcttgtcac tagctaattc
      541 cttgatattg ttgatataac tatctaaatc ctttaatatc attaaatttc cttggtttgt
      601 tagctcctgt ttccgtctgt ccatttcttt cctaacatgc tctttattca tccaattata
      661 gattgttttt ctatttactc caataattct ggcagtttct gttactgtag cacctgtaat
      721 caatagagtt accatattgc actgatcttc tgtcaactct ttaccttttt catagctact
      781 atcgattatt tcaacttcat tcacatccaa ttaatcacct ctttactcag cttctaaata
      841 aacatttaaa tatctacatc cctataaatc ccttgaataa atgaccttta atatatattt
      901 tcagacattt taataaatct attctattcc ttgtaaactc tattgttaag gctctttgca
      961 ttctaataat gtcctgttgt tgaaaaacgt aacatattaa attttagata aaatacaaga
     1021 tattttaatt tctttttctt ttctttaact tattagtaaa tttagttatt gcttttactt
     1081 cttcatcctt atctattcct atatatcttc tagtcatttc tattgtctta tgtcctagaa
     1141 tcttccttat aagttctaat gcataagtct tattctcttc ttcatcataa atatgccttg
     1201 caaatgtttt tcttaaagag tgtgttgcaa cattttcttt tattcctacc ttttttgctg
     1261 ctccttttat tattcttcct gcttgtcttg tttggatagg tctattaaat ttcttcctct
     1321 tgccactttt aaataaaaat tcttctcctg gtttatcttg agtataattt tctatagcac
     1381 tttttattac atcagataaa aaaacctctc tattatagtc tcttttttct tcgataactc
     1441 taatccaatc cttagatact cttattttat ttcctttttt atcgagtttt aagtctgtta
     1501 catctgcaac tttaagtgct aatatatccc ctaccctaag tccagtacac attccaaaga
     1561 gggctaataa atagtttctt tcattccatt cttctagaaa atttaaaagt ctaaatacat
     1621 cctctatatc ttttatggga ttaacttttt gtccactatg tgcttccatt cattaaaaac
     1681 actccttatt tccataataa ttttttcttt gcctctcttg aactaatatt tttaagtcct
     1741 caactacatc agtaaaagga tctgtgatta tagaggttat atcatttgta ctatattttc
     1801 tttctcttgc ataagtgtta gtctttattc taaatcctaa gtcgtttaca gtttcagtta
     1861 cttttacaac attttctaat tgcaaatata atttaaatac aaccaactca acgtgatcag
     1921 actcatcaat tatacttttc tctaaaattt ttagatatga gtctatctta ttaatattag
     1981 aatcataatt tttcttaacc ttctttttta gtttcaagag tttttcttgc tttttcttta
     2041 taaaatctaa agaacattct cttccataca taccaggagt ttctttcatt acattgtcgt
     2101 aatactttag attgtctggt acatatgcag cacttgaaat ttcttctgtt atatcacgtt
     2161 taatcttcaa cacctctcta gacaataaaa aagacttaga acgtaaattc caagccattt
     2221 ttattaaata tatttttctc acaatacaat cttatcatga ttaaaagaca caaaacagac
     2281 ataaattggt cacaaatcgg acatttttta gacacaaaac agacataaat cggtttaaaa
     2341 tcgtcacaaa tcggacattt ttatagcaat ccttttatct tatctataat atcaaacctc
     2401 atttgcttac acttactcgc tgaaaaacca atcattctgc caacatattc ccaagatgtc
     2461 ctgctacttg aaaagtattt atactgtact atcttttttt cttcttcact taatattttc
     2521 attgcattat ctattttttc tattatcaac tctttatgtc tcttctcttt ttcttttaag
     2581 gcaattttct tttctttttc ttcagtctct atttcaatta cacttttaat atcatatgtc
     2641 ttgcttgtct tttctttaat ttcaactcca ctacatcctc tgtagtcgtt ttttatcatc
     2701 tgcatatcaa tgtctatttt ttctatttcc gcttttattt ttttataatt atataactta
     2761 ccttctgcac tactaaacaa attgtctttt ttgctagatt gcattacctc acactcctaa
     2821 ttatgttata atgatgttgt gatgacggtt ttagaatttt gacaagcagg agcgtgaagt
     2881 aatgctcctt tttttctttt ttgaaatata tcttattctc taggcactaa ttgcaacttg
     2941 taatcatcag aactatataa tttataacct cttaccttca agtctaattc tagttgactt
     3001 aaacttttat aagcaccaaa tgaaccatat ccattctgca tatttataat aacataccca
     3061 cctattgttt cctttaaaga aagaatcata tataacttat taccaccatt aatttcaaca
     3121 atatctccta cttttaattc tctattattt tcagttactt ctatcaacat attatatcat
     3181 ctccttactt taattaatat tttttagaaa ctctcccatt ttcatttcat caatgttttc
     3241 attaattcct ttcatagcac ttaagtcaac caaaggcacg cacaaatccc atagcttatt
     3301 tacatctaaa aattgtattt ttgaattaaa ttctttttca tcaacgcatt ttaatttatt
     3361 attcatatta attaattcat taatcattga atttttaatt aaattaagta taccatctcc
     3421 tatttctttg agatcccatc ctcttccatt taatttctta ttatgtatct ctataccttc
     3481 tgcaatataa tcttctaatt ctaaaatttg aaacattgtt aaaatagttt ggcaaacatc
     3541 taatccttct gcaattatat ctttagtcat tcctgtatct atagcctctt taaactcagt
     3601 tacttcttca tcaatttttg ccaactgatc ctctaccttc caattaggta tactgctaat
     3661 taatttcaaa tccattttta atatctctcc tctattattt attgaagtcc caactctttt
     3721 tgagtatatt tattcataat gctccttttt tcttttttta aataataact caatctcttg
     3781 ttttttggga acataaatat atttacttaa tcgctgcatc tttttatata aagaatcttt
     3841 tatttcttcg tagtcaaatc cattgaaata tcctggaagt tttatttcca actttgtggc
     3901 ataaaactca tacagcattg cagaaacaaa cttaagatca ttaataatat gatttgcttc
     3961 aaataacatt agatttttat tgtaaaatcc tattcccatg gcaacataaa gataaatata
     4021 ttcattcata aataattctt ccacaatatc tacataaact tccaataaat catttatata
     4081 tcttatctgt ttttccgttg catcattaaa tagatattgt ttattatttc gatgaacttt
     4141 atagtgacaa ctattgcata atggaattaa atcatccaat tcttccgctc ctattcttcg
     4201 atatgtttta tgatgcaaag acactttctt tactgaatca cacaaaacac atttacattc
     4261 attagaaaaa tagaattcat tttttagtga ttcccagtgt ttagtttttt gatattcttt
     4321 ataattatag acaatctcgt ttgtatcttg acatactatt ctcttattca tttattgaag
     4381 tcccaactct ttttgagtgt atttattcat aaatatcatt tcatcacaac tattaaatat
     4441 aaataagtct ccatctaata taaattgcaa tgatgcaata tcttctatat cagttaaatc
     4501 catttctcca tttaagacat tttcattatc tatgtcattt aattcaaaga atatattttc
     4561 tgtttcaaat ctatcaatta actcatttgc atctttcata tttttaatag catgatacac
     4621 tttacatttc atgtctgatc ttctcctctt acatctttat ttatcttcaa gccaatcatt
     4681 ttttaaataa taaaatacat atataatgat tgtagttatt aatatccaga taatccaaaa
     4741 cactacttta aaaaatccta tattaaaatt gttattttcc aaaacattct gtatagattc
     4801 acccttaaaa acttttatgt ttttatcagc tggattcgct atagtattat tttttaaatt
     4861 agcatacatg gtaccattaa attctttcgg tataacttca taatagtacc ttctcttact
     4921 attaatgtaa gcataattac cttttatttt aagattagta tttttaaata tatctttctt
     4981 taatggcaat ctctctttag ataaattagt aattactttc acatcaaact ctttacccat
     5041 gaaattaaat ttattacatt tttttctcta ttgaatcatg gtaatcccaa gtccaatatg
     5101 tttcagtata tttatgtgaa tggccttcac tgtctttagt ggtaaccact ctagtatgca
     5161 tagtgtattt ttcttccgtt ctttctataa accaatagtc cccttttaat tcttctatag
     5221 atacagaatc tacactttta aatcttccac ttatcaaaga atctcccgca cttgttttca
     5281 tagcataatc aaagagatct ttgtcattat ttattttagt tgctttgtaa tatctttcat
     5341 tatcttctaa gaattttgat tgtactttat catcaatgaa aaatccaaaa caaatcattg
     5401 cacaaacaat tattatgctc actaaaaatt ctctcttagt tatttcaaaa gttttaaatt
     5461 tcatatgtgc acctcttaat caaataaatt ttgaggtgca tcctcttttg ctttatattc
     5521 agtatattta gattctattt tttcatatcc catactattt agaattaaat ttgctggaaa
     5581 ttttcttata tatttattgt aattgcgaac ttgcatatta taattttctc tatgttgtgc
     5641 aatcatattt tctgttatac tcaactcact cattagttgc ttataatttt cattagcttc
     5701 taaatctggg tacttctctg ctactgcttg tatatttaat tttgcttcct tgatagattc
     5761 atcatttgca cttactctca tgtcagttac ttcttttaat gttctatttt catatttagc
     5821 atagtctttt acgcagtcta ctatgttctt aattaaatca attctccttt tttcttgcac
     5881 ctctacagat gcagttgctg attggacctg ctcttctaaa tcaattgctt tatttttagt
     5941 tactaagaac aagcaagcaa gcataattaa tattgaaata actactccta cgattattaa
     6001 cgtctttttc attatttcat cgcctcactt tcaagccatt tttttctacc ttctaaacat
     6061 ccgcctaagc attcttgaaa atcataagca caactagtac atccacttcc gccatttgca
     6121 agaaattcag ccatttctat tttgctcata ttttttatct tttcataatt agtcatcttc
     6181 ttattcaagt tacttccaaa taatctgtca taacatttgt cacatacatc ttttccgata
     6241 ctcattatat taccttcatc atcaattctg aataccttta tgtttagaat ttctgctatt
     6301 gtttcaccac aaaaatcaca ctttataaca caactcatct ttaatacccc cttttacttt
     6361 ctttagaact catatcttga tacatacatc cacaatcctt acattgccac actattccaa
     6421 ttaatgtttc aatcttaaat atattaccat tacaaattgg acactcattt tccctaccac
     6481 caattatttt agttttcatt ttacctccaa taaacttaaa tcatttatta tttacctagt
     6541 atattaattt cagtttttaa ttttttaagt ctcctatata ttgacgcttg gctagttcca
     6601 agcactttac ttatttcttg ttgagtataa cctttttctc ttagacaaac tattttctgt
     6661 atatctttta tctcacttct ttttatacaa gatctaataa cacgaacatt ttctatatct
     6721 acattttcat tgccagccaa tctatcttct attcttatct cttctccata attatctcta
     6781 taaatacact tttcaaaact atctatatca tttctataat gtttcttaac atatccagtc
     6841 gtaaatcttt tcaaatgtcc tgatatatac aaaaatgcta ttgtagaaaa ttttccttta
     6901 ctttcatcaa agcaacatgt tgttttatat aatcctatat atccttcttg aaataaatct
     6961 tcttttaaat atggatacct tcttaaaaag gatttgaaat atctatttat aataaaatgt
     7021 acaagattta tattatcttc aaataattta tttttctctt ctaaacttct catataatca
     7081 ccatccaatt taaaatggaa tatcttcatc atctaaaact tgaaagccta tttctttttc
     7141 ttcatccttt ggcttgtgat caagcgcatt aacaattttt gttcttactt ttgtgaaagt
     7201 ttttttctct ccatcttttt catatttttc tatccttatt tggccttgaa ttgaaaccat
     7261 tcttcctttt gcgagataat tagcacaaaa ttctgctgat cctcccataa cttcaattgg
     7321 tataaaatca acttctctag ttccatcctt ttttgcataa cccctatcaa ctgccaatgc
     7381 aaaagtagca actggagtac catactctgg tatatatctt aattctggat ctcttgctaa
     7441 tcttccaact aaaactacat tattcatatt gcaatatctc cttcattgta tttattttct
     7501 ctatattcca tttgttttac aatctcaact gcttcattaa aggcacaacc tctttcaatt
     7561 tctttatttg cagacatgat tgcattagct attttatctg taaattctgc tctttctaaa
     7621 atgttcatat gagaaatcat tttcatctaa tcccctcctt ttttactaag aggacttatt
     7681 gtaagtcctc ttagattttc aattaagcaa ttatatttac atttttaaaa tcttttaatt
     7741 cttcttgcaa gtacgcttta atgtttaaca ttgcttgatt tttccatgct ccaccatctg
     7801 cttcatataa tcctgctgct ggtccagaac tcattctgaa aataaactca ctttctggtt
     7861 gctctatctc ttggaaagtg cgataaggaa ttaactttac tctgtttggg actattgctt
     7921 ctgatacact agctacacct gtttttattg ttacagactg agaaactcca tcatctccca
     7981 cattttttac tgtagattct tggatatttc ctgcaatttt taaaagtgta tctctatcat
     8041 tattctttat aaatgctgat tgtagcatta tgttaaaact atctaagtct aaaggtctgt
     8101 caaatctttt ctctggtagt agtgcaacac attcaatata tgtctctcta tctctatttt
     8161 ttctcaaagg agattttaaa agcacttttc ttggagttag aacttgtatt aataacaact
     8221 catctgcctt atgatctatg ccacttctta tataatctac taaaccactc aatgtagtta
     8281 tttctaattc attagctttt ggttctttta ttatttctaa tgcatctgtt gtgtacttaa
     8341 acccatctac acatactgtt tctctttttc ctaacttaac taaatattcc attgcatttc
     8401 ttatcatttt tttattctcc ttttttattt aaatttatat tttacttact ttcttaaact
     8461 actttgaact tgcatttctg aaatcaatta cactagcact attttcttct gtttcattag
     8521 tttcttcact atctggaata tccatgacca actgccctgc tacttgattt ttaaattcag
     8581 acgcttcaac tcttcctgtc tctaaatctt ttcctatcag aactttagtt gttgtagcct
     8641 ttgcttctac tatagttgat ttggatttta tatctacttc agcaagatct ctatcttcat
     8701 ttgctttaaa cactatttct aaactaattt ttcttgtttt tttaaaatct gtatttggat
     8761 ctgcaatgtt atctaaaact ttttgtaact ctgtattaaa cttttctttt agaactccac
     8821 ctgcaaattt ttctaatgct atcataaaaa tcactccttt atttatttaa aataatattt
     8881 ttctacgagg aaattaatta ataaacttta gctctaattt aataacactt ttaaaacttt
     8941 tataaattta tatagccttt ttatccttac actactttta agttttttgc tagcatatct
     9001 aaacctgtac gtattatgtc aggaaattaa tttatataat cgttgcaaat gctatatttt
     9061 caaacttttt ttagtattga aattcaatac tttttcaaaa tccgattttc aatattgaaa
     9121 aatgattttt ttcaatattg aatttttaaa ttattcaata ttggtttttc ttataaaatc
     9181 attatttaat aaaatttcct attttttatc ctttttttta aaatacatct tctggaattt
     9241 ctacttgaac atattgttta tttacatcat atttgtaatc tctgtttagc tcctcactag
     9301 tcgtgtaaag tctttttctt ctttctgaaa aattcatttt aaaacttata ttctgttttc
     9361 ctgttattct attttttaaa attaatagat aagtgtcgtc tgtttcttct tcttcgtcaa
     9421 taaccctgta tgttgataaa atataatctg cataattaac aatctcactt gctccagaca
     9481 catcatacat agttggcttc atgtcaccca tatttttacg aggatgtgca actaaaacta
     9541 ttactaagtt atatttttta gcaaggtttt ttaagttgct tactatctgc tcttgtctct
     9601 catatttatc tgccttattg ccagttttta ttgtcattag attgtcaaga acaaagaacc
     9661 taaccccttt ttttaaatat aaatactcta tggtatttag tagatttact tcatcactta
     9721 ttgcatcttc gtcatagaga aaaaatttat cttttatcca gtctgaaata agttcagagg
     9781 cataatctgg aatatctgtg tatgttcctc catagacact tttatattct tttatgtgat
     9841 aatcattagc tacagttttt ctaaaccaat ccatagccat gaaagagggt aattctccag
     9901 aatacaaaaa tgctttttct ccattcgtaa ttgcttgtgc tataaactgg ttaactatag
     9961 ttgatttacc agacgcaggt ttaccagtca aaattgtaag agttccatat ctaaatccat
    10021 ctagcatatt gtttagttta ttaaaacctg tgtctatgga cttgtgtacg cctttttcac
    10081 gctttatttt tgacgcatca atgtaattat catttatgcc actgctcgtc gttacaagtg
    10141 cttttaaatc gtttatatct acaatcaatt cgtctatact tgtatctgaa tttgaattat
    10201 taatcaattc atttgctaac tctgaaattt gtcttttaag taacttttct tttatgattt
    10261 ttatatagtg atctatgttt gaagtagtta tgactatagt tgttaaactg gtaatgtaac
    10321 taactcctcc aacgtctact aatctatcta ttttttctag ttcatttttt aaaagcaaaa
    10381 gatctaaagg tttatttgag tcaattattc tcttcataca agataaaata attttatgtg
    10441 actcaaagta gaaatcattt tcttttaatt cccttatttt gtaacctata gagtcattta
    10501 taagaaaact tcctagaatg ttttgctctg cttctaaaga gtaattcatt taatcacccc
    10561 cagcttatta ttaaaatgtc acaactaaag gaatcaccct ttcgacgtca tacaataccc
    10621 cacaaatacg atctcttgga acatttttgt aaactaaaat atcaattatg tcggcaataa
    10681 gttttgcata gtattttgga gtttcaaatc cataacttct ataagcaagt ttttcttcaa
    10741 caaattgttc gcatttactt cttgtacttg ttctagtttc acaaccttta ctttcaaata
    10801 tcatttctct tatttttgga tcagttctta atccttcaat aagtctttgt atatcttgat
    10861 ctgtaagttc atgttttttt ctttccactt agcatacctc ctgtctttaa ctatttagtt
    10921 gcttcttttg atttgaaact attctacacc atgataattt acatgctata attaatttaa
    10981 acatacttcc aatatgttta aattaattag aaaggtggtg agagtatgag ccaatatcga
    11041 gttataagca aagaatgtcc tgtaataaac gaaaccattt ctcttacttg cgaatattca
    11101 gctcctccag gtacttttgg atctaagtta atgtcaacta gatgtgatgg aagtcatatt
    11161 tgcgactctt cttctaatga agaatgtcct attctaaaaa aacttcttag atagtagctt
    11221 tgggattttc atctggaaaa caatacttaa atgcagtcac ttttacacct gttattttgc
    11281 ttaatttagt aaaagaatga tacatacagg taaaatctcc ctcacattct tgcaggtaat
    11341 cgcaaacttc acatgatgtg ggggcttctc tattccctct aaataccatc tccataactt
    11401 cattttgcaa atgtcttgca atacaatgca attctttttc agtaagttct aacttttcct
    11461 ctttcatgaa caacccccta atttatttaa catttaatta tttatctgtt tcattgattt
    11521 tataactaaa tttaattgac ctatgcttta attctacacc atgataattt gcatgttata
    11581 attaatttga gcatattggc agtatgttca aattaattat agggtggtga tgaatatgtg
    11641 caaaattaaa accttaagag aaaaatgctc tcttattgac gataaagttg aattttctgg
    11701 tgaatataaa acttttcctg gattcgctgg atcaaaactt ttagagtgcg aatgtaataa
    11761 aaattacatt tgcaaaattc aaataatgac tgctaatagc aactgtcctc tttttaaaaa
    11821 agcacgtcaa ttacaatact aaaattttaa tttactaaat ttatttagta aaaatatcta
    11881 cacctgtcat ttcacttaat tttataaaag tgtctatatg ggtgaaatag cccttgcatt
    11941 cttgcaagta atcgcaaact tcgcatgatg taggggcttc tatattccct ctaaacacca
    12001 tctccataac ttcattttgt aaatgtcttg caatacagtg taaatctttt tcagtaagtt
    12061 ctaacttttc atctttcata ggcaacctcc taatttatac ttaactaaat atctgactta
    12121 atatcgaact taatattaaa ttgggcaagt ttattaatcg attcttggag ttgacaaatt
    12181 cgctcattga cattttctat caattcctca aattccttaa tatccctaac acctaagaat
    12241 aacttcacat tagaactatt caaataaaca gtctcttctt tttcattcat cctatctacc
    12301 tcctatacta tttagttttt aaagttcttt aaagccacta agtggattct tagcctttgt
    12361 aactgctttg ctagtatcag cattccccca agtcaccaaa gccctgtaat gacttttata
    12421 cctactccct tttccattta caatatagtt ttctaaacta acaattttat catgtatgta
    12481 tttcttccca aacttgctta taagtttgtc atattcctct ttagttagtt ttactttttc
    12541 tatatctaag tccaagaatg ataggtctaa ataatcagtt ttagattctt tttgtgtagt
    12601 attacttttt atataagtct ttttattata agtcttttta ctgtgcacct catgcactac
    12661 ctgttgtgca tgaggtgcac tagggttgtg catcatattc actacctgtt gtgcatcaga
    12721 atcagttcta tcatttttaa gtgatttttt tgattgtgta ttattactta tattagtatt
    12781 gttaatatta gtattgttac ctatcacctg gtgacatacc cctctatcat ctcctgctat
    12841 agggtatgtc atctcgtgat atacccccct gtcatctcct gctataggta tatcatctcg
    12901 tgatataccc ttggcactca taatgcaata taaattacta tcattttcct tttcattttt
    12961 ctttctagca gtctttttta taagttgttt ttcttctagt gcatttactg cctttctgac
    13021 tgtatttatg ctacatccaa cttttttcgc tatagtttga ttgctaggaa aacacacact
    13081 ttcactattt gcatgccgag ctagtactat atatgtcatc ttttcatata catttaagtt
    13141 taaattatct atgagatcat tttctaacca gaaccaatcc ttttttcttg catctctcaa
    13201 gagatccatt tcatcacctc ccttctaaag ttatttatta atttaaaagc tttagtttgc
    13261 aatcgccatt ttctaaatct tcataagtta tagctttata tcctgtgtca tcattttctt
    13321 gcatgtaaaa cattacaaca atttcgccta cttccacttc tgtgatgatg caatctgtta
    13381 tggtattaaa tcttatttct tgtaattgaa ctgctttacc tatgtggttt ctattaaaat
    13441 ctgagatttt caatcttttc acccctttaa ttttgtatat tattttcatc ttcaatgtct
    13501 tgtatttctt ttaatatatc tactttagtc aatgctaatt ttagttttgc agggccaagt
    13561 aactccaatt ttctatatat ttcttctcta attttattta tatcagtcat tttcaatcac
    13621 ctcgcttttg ttgattgtgt aatacaatta tattacatct ttgcttacaa gtcaactatt
    13681 ttgtaataca tttttctttt atttttgttg acttgtaagc acttttaaaa tataatattt
    13741 catatggagg gataataatg atagatgaaa tgaaaccaga aaacaaaaga ataagagagc
    13801 taagattaaa tctcaattta agccaagaag agtttgctaa taaactaaat ttaaaaagaa
    13861 acacaatatc tttaattgaa aatggtaaaa gaaatgcaag cgaaagaact ttgaacgaca
    13921 tttgcaagac ttggagcata tctgaagaat ggcttaagca tggagttgga gagatgtttt
    13981 ttatcaaaga attagatgag caatttgccc atcttgttgg tgaattaagt aaaatggaaa
    14041 atgaagtaga aagagaaatt ataactaaaa tgttcaaatt gaataaaaaa tatctgactg
    14101 tatttgataa aatgatagat tccttactag aaatagaaga atagcaattg ctattcttct
    14161 atttttttaa aagtccaagg atataatttt ttatcatcaa taaattcctt ttatctttat
    14221 caatatttaa tttttttata atttcaatta tttcatgttt ttccttttct aaatccctca
    14281 taagtttatc ccccatattt tcaaaatatt ctgatttata tttttgcaat tacattgctt
    14341 ctgaaaaata acagacttcc cccttttaca atttttaaat ttttatcagc tactaaatta
    14401 atgtttttgc ttttgtatgt catgccttag atgagtttga aaatttgatt gtattcaata
    14461 ttgaaaatta agatttttga ttatttttaa aaatatttta cgtacgtagt tttatatgca
    14521 ttttatatgt aatttcgata tatatcttct ttatgttaca attgtaacac ctcatttaac
    14581 attaatcaaa tgtcaaattt taccaattta aattattttt taaaatttaa ttttacatta
    14641 atagaattta tttatataat aaattaaaaa aggactatga aaaatagaat tttacttcta
    14701 tagtccatag tctttccata atttatgatt gatataaatc tgttattttt agaaaccagt
    14761 tatttctgat aactcataag atgcaatttc tccatctgta ttcattccca ttgctttgaa
    14821 cttccaagta ccatcttttt taagatcgtt tatattatcc attgcagtac ctagttgatt
    14881 tccatcctta tcatataaag taaatgttac ttgtgcatat gatatatcat gaccactgtt
    14941 gtttttaata acacctttta tatacgttgc catactatca gtttctgatg ttatatctcc
    15001 aactatttca tatttttcct ttttaggctc ttctggttta tctacactag ctgttttatt
    15061 tgttccatca tcattacctt tagcagtatc atcagttccg ccacttccac tagtaccaat
    15121 tgcgtataat aaaactaaaa ctactataac tataaaccaa cctcttttat aaaatggttt
    15181 tttgttttta gccccacagt taggacatga tttagcattt gatgctattt cctttgaaca
    15241 tgatttacat tgaatcattt ttgacatttt aaaatccccc tccaattagt ttatgatgac
    15301 ctcaatcata ttatagtata caactaatgt ctaaaactgt aatatcatga catcaaaaga
    15361 attttattag gtatatggca aatattttgt tgtactaaat ggcattcaat aaagactttc
    15421 acttatattg tatacatttt ttttgaactg cttttaagtt ataatattaa ataagttatt
    15481 tatttaatat tataatacgt ctttataatt agcatattaa agtatttttt ataatcagaa
    15541 ttaattggaa aaaattggaa tacataatta gacatttttc attgtaaatt aaattctatt
    15601 gtaatagtat tatagtatat caaaaaaacg ggggtgtagt acttgaaaat aaaaaagatt
    15661 ccagagtcag aacttttggt aatgatgtac atttgggata gtgaagatga agaagttgca
    15721 tctactgaaa ttttgaaaac attaggcgaa aaatatgaat ggaaaaaatc aactatgcta
    15781 acttttttaa ggagattagt tggtagagga tttttagagg ttgttaagaa agatagattt
    15841 acatactaca aagcattaat agaaaaagaa gaatatttaa aagttgaaac taaaagtttc
    15901 tttagtttct ttcacagaaa ttcctttgaa agttttataa gtgcattaca tgatgatgaa
    15961 gaaatatctg caaataaatt aaaaagttta gaaaagttta ttgaggattg ggaagaagat
    16021 aaatagagag ccaatataat tttatttggt tctctatttt tatatttgac cttctatttc
    16081 catttttaag caaatttgta tctttttagg taaataatat caagtttgca attaaaacgt
    16141 gtcagacaag cctttaaatg ccttaaaact atacttaatc aactttattt ttatcaattt
    16201 cttttttccg atcatcaaaa ttacaaattc gataaacttt tcttattttt ggaacttctc
    16261 taagtttttc tttgtcatat tcatatacat ttactttttt tgtttcaatg tatttatttt
    16321 cttctaaagt tttaaccgaa gaagttatgt tttataagaa attattttta gtaagtgttt
    16381 aaagtatact agaaaaataa atactaaagt tttttaatat aaaaaaaaga atagttcaga
    16441 acatcttttc tagtctttaa tagtttttaa aacagttttt aaagtcttta atagttttta
    16501 gacaagctat aataattggt atatctaggt ttcagagatg aaaggtcatc attttaaggg
    16561 tcaaaggtca tcattttaag ggtcaaaggt catcatttta agggtcaaaa ggtcatcatt
    16621 ttaagggtca aaggtcatca ttttaagggt caaaggtcat cattttaagg gtcaaaggtg
    16681 agtgtatttt aaaaatagtt gaccttttta taaattaggt attataattt atatgaggtg
    16741 atagattttg gaaaaaaaag aaattttaat gcaaccaaat aatttaataa aaagtaaata
    16801 tgattttaca aatgtggaaa ataaattatt ttacaaaata ctttttaatg ctcagaaaca
    16861 gcaaaattca tcttatgtta caactatatc aaaagaagaa ctaaaagtat ttatgaaaaa
    16921 caataatgat tatgagcata aaaatataaa agaaattttg aatatgtttc aacagagtgt
    16981 cttggaattt gactatatag aagagacaac tggcaagtta aaaacttttg gaagtggttt
    17041 aattaataca tatgaattgg atcatacaga ccaaatttat acaattatga tgcatgaagt
    17101 tctatacaat catataactg attttgtaaa aatgcagaaa aagaaaaatg gatatacagc
    17161 tattaattta tcagttttat ttaattttag aggtgcatat actcagaggc tatacacttt
    17221 atttaggcta tggagcagag aaaataagga agtagagata aaatataaat tagatgaatt
    17281 aagattttat ttgaaattaa aagataatgt atatccagaa tataaatatt ttaagcaaaa
    17341 tgttctaaaa agagcaatga atgaaataaa taaaaaaggc aacatggttg tttcaataaa
    17401 agaagaaaaa agaaaaaata gaaaaataga tgagataatt ttttctgtaa tagattatga
    17461 acctagaaaa tattttgata aagatatatt ggttgaagat tatataccta aaaagaagca
    17521 ggacaatgaa gtgtctttta gtttttatat accaaatgaa aatattttta caacagggac
    17581 gattaaacta tttaaagaag attttaaaga gtttgatttt aagaaagatg tttatctgaa
    17641 tgcttttaat aagtctattg ctatagcact tgaaagagat aatactgata ttataaatac
    17701 aaagaattat gattttttta aatcaacact ttcaaataaa ataagtgaag ttataattca
    17761 tcaaaaagat gatttaaagt ttaaagaaga attggataag tattgggatg atgaagatgt
    17821 taaaaaagaa gaaagagaag ttgattatag caaagatcct gtcagacttg aaactgttag
    17881 gagaaattta tttgatcaag gtctagtaaa tgaagaagga tattactata aataaaaatt
    17941 ggagaaaata aaaaatgtat tatgtttata aatatattga tccaactaca gaagaatgtt
    18001 tatacgttgg aaaaacagaa gatatatatg caaggcattc atctcatttg agtaataaag
    18061 aagaagactg gtgtactaaa gaattaatat taaaatatat tgaacttcca cgtagatata
    18121 atatggattt ttttgaagtt tatttaataa atgaactaaa tccaatattt aataaactaa
    18181 gtaaaggcga tatggatact gaacacatct tttttgatta caaggaagaa gattggagta
    18241 catattcaga agaagatttt aaggaaaaga caaagggaaa aagaagattc agagaaagaa
    18301 agatagatat gaaatatgaa gtaagtaaaa aatctattaa ttttttaaag agaattgaaa
    18361 agttagatgc caaggttgaa atgttatatg aaaataatgt tttaagtgtg ctatataact
    18421 ttgaaaatat ggatatagaa ttttgtgaga atgaattaga tattaatttg atatcatatg
    18481 attttgaaac attagaaagc tttggaagtt gttctttgat ttcagtttat cctagatgga
    18541 aagaatatac agcaacaaat aaaaaaatac ttatagatat aacattagag attaggattg
    18601 gatctttggc taatttaatg gaattaatgc caacatttga atataaaatt gctgagattt
    18661 tccaacaaat agaggatatt tttcacatac tagaagttgg atatacatgg caagaattat
    18721 ttgaaatata taagggctgt aaatatgaca attaagaatt ggagaaaata aaaaatgtat
    18781 tatgtttata aatatattga tccagctaca gaagaatgtt tatacgttgg gaaaacagag
    18841 aatatctgtg atagacatgc tagtcattta agtaataaaa aagaaaattg gtgtaataag
    18901 aatttaagac ttgagtatat ggaattagat aataaatata ctatggattt ttatgagatt
    18961 tacttaataa ataaactaga tcctaaattt aatgttactg gaaaaggaga aatggatatc
    19021 gctaaaacat cattttccta taatggtcaa tgggtaattt attcagaaag agacttgatg
    19081 tcgagtttgg catcaaagag atgtggaata aactatgaag tgaatgctaa gatgttaggc
    19141 ataatgagaa aattaaagaa gataagcaac aatgaagaaa tatttattgg aaatgaaaat
    19201 ataaaaataa aatattcttt tgaaacaaca ctagaaggaa ttgatttaga gccatgtata
    19261 ttgagtgtag catataagaa actaaaagaa agtacagttg gaacattgat ttcagtaaaa
    19321 cgtgaatata tgccagaaaa tgtatgttta atcattgata gtatttttct aaatgaaata
    19381 atgaaagatc ctttaatgtc taaatcagat attaaagatt taaatagcca agtcaatgat
    19441 attcttaaaa taataggggt tgattgtgag tggaacaagc tttctgatta ttgcagtaca
    19501 aactcttaac tgatattaca aaatatggta taattaaatt tagcaagaag agcgtaatat
    19561 acaatctata gagtggagtt catgtacaaa agattatcct cccaacgatt cgaggggagg
    19621 tgagttacat ggataacttt ttgtttaatg ttttagctag tttaacagct agcgtggtag
    19681 tttacttaat cagtaaacta ttcaaaaaag caaaaagcca ctctcgtgca aagagtgact
    19741 tacaggttga atttaaattt atatttaaat tcaaaaaata aactatgttt attatgaact
    19801 ccactctacg cctaaataga ttgtagttct tcttgctttt attatatcac aaaatcttta
    19861 aaatgataca attttatatc atttcttatt attaagaaat agaaaaacac tttaattgat
    19921 taaagaataa ttttatataa tcttgtaaaa atataaaaag aacacttaat atattagctt
    19981 gaaaaatatt aagtgttctt ttttttctat tctatttaaa taaaatagaa tagaaaaaat
    20041 tattgaaaat attattctat cgtgatagaa tgaaaaaaac agaatattct accgtgatag
    20101 aatattctgt tttgaagaag aagtaaatat tatttgaagg ttaaaaatat aagaacaatg
    20161 gagggaatta tggaagtaaa cctacataca ggaaaagatg aattttttaa gagccatgaa
    20221 gtagcaaaaa actatatagg tggattatat aaacttattg atgtttacaa attctcaatg
    20281 gaatcagttt ccgttttaac tagaatagat ttagataagt taaataattt ctatgctgga
    20341 gaagctagtt taaactatga tgaattgtgc ttaatagaaa gtgtaattat aactcctttg
    20401 gtaggagcat ttgaaactgc tgagcataat tacaatatga ttattaaggc acgtaaagaa
    20461 aggaaactgt aattgagaat aatataaaaa aaataggggg aaaacaaatg aaaaaaacaa
    20521 ttgctttatt actaacgctt tgtgcagtaa tgattccatt tacagctttt gctgatggaa
    20581 atgattatga aggtactgga aattcacaaa cagttgaatt aattggccaa ccaaactcag
    20641 attcgccttc gactaaatct gttgttgttc caggtggtac tgctaattta acagcaatga
    20701 gtggaggaca attacattgg agagtaacat ctacgcttgg aaatatttta ggatttgaag
    20761 gaaatataga tgtatataag gctggcaaaa ttgtatcatc tcaatttgta agttttagat
    20821 tagctggaac tactaaaact ggtgtagtaa gtttcaaagg gctaaaaaag ggttcatata
    20881 ttgctaaatt taaaggaaaa ggtgtttgta caggaggtat tatattattc tctagtccag
    20941 ctgaagcttc ttttacaatt agataattat aaaaagtaga aaagagtgtt tagaaatata
    21001 tgaaacactc ttttttttat gcaaaattat aaaaagtgtg tttttaatta tatacaaaat
    21061 gataaatttt ataaaaaatt ataaaattac ataaaaaatt ataaaatatt ataaaaaata
    21121 tcttatgaat ctattgaatt actatgtgaa atttggtaaa atatatgtaa atattaataa
    21181 attttataat attttacaaa aaaggaagtg gttgaatgag taggatagtt ctaacattta
    21241 aaaacaataa taaagaaaaa gcaattgaaa agtttttaga tgagaaattg tcagctacag
    21301 cttatttaaa agagcttgtt tgggaaaaaa tgaatgaaaa aaaggataat gctgtagttg
    21361 aacagaaaaa agaaatagaa gatccagcta acaattttga ttttggaagt ttagaataaa
    21421 agggggaaat aacataaatg agtaaattag gtatagatat tggaaattat gcagttaaaa
    21481 caagtacaga tgatattttt gaaagtaaag ttacagaggt aaaaaacttt ggttcagatt
    21541 cagatagcat taaaataggt aataaaacat attatttagg cgaaggtgac gaggaaataa
    21601 atatagttaa atatgagaaa gaaaacttct taccgctttt acttggggct atatgcagaa
    21661 atacagatga tgaagttatt gatctagcat taggtctacc agtgaagcaa tttggaggat
    21721 taagaaaaga tttgattgaa aaattacaag gaaaagaata tcatgttgaa tttgcaaaag
    21781 gaaatgaaac tactaaaaga gatataacaa taagatctgt tcaagtgttt ccagagggag
    21841 ttacaggcta tttatattat gcaaaagata tagttgacca agttgcagga agagatgttg
    21901 ttttagttga tattggtgga aagacaacag atattgcact tgtacaagga aataaagcaa
    21961 ctaatccata ttctataaat gtgggaacaa taaatatata tgatgcaata aagaaatctc
    22021 ttgagatgga cgaaagattt ttaggtaaag ttgagataaa aagagaaaaa atacaagact
    22081 acataaataa aggattttac ctaaatggtg aaaaacaaga tataaagaaa aatatagatg
    22141 catctatttc tctatttaaa gaaatataca atgaattgaa attaaattat ccagttagta
    22201 cagctgcggt tgttgttatg ggtggaggtg ctaaattgtt aggtgaagca tttaaaaaga
    22261 atatacctgg cataatagtt atgagtgatg tagataaaca tgtttttgca aatgcaaaag
    22321 gatacaaaaa aatgatgaaa taaaaaagga tttagtttat agtatgtata atagattgca
    22381 aatatatgat atttgtaatc tattattttt tttgaggagg atttatatga cttggagtga
    22441 tttgaaagaa aaaaatatta atgaagttgt tgattaaaaa gtagctacgc acactcgtga
    22501 ctttagtcat gagttagtag ctaaaatagt cagcatatag ggaaacttat atgtagagat
    22561 aggatagaac ctatccaata ctacttgaat tgctggaaac ccttgcaatc taaaacattc
    22621 tcttggagtt aatcttctta ctctaaattt tttgccaatg acaccagcat tacttgcagt
    22681 agtcaaagtg caacttcttt tgtgttgaac tcttccacgc cccgttttgc ctcctatatg
    22741 atctagtctt atgccatctc ctattgcact acttctatgt tcttttcctc taaaaccttt
    22801 atcaaaatca agtctgatga atttttccat tttttatatc ctcctttgtt tatctctttc
    22861 tttaattata ttataatcta tatgaatatc taagtcaaca ctttttcaaa gttttttctt
    22921 tgatttccaa aaatataact ttgattagta tagattattc ttttactttt aattttttta
    22981 tgttattata taaataagag aggtgcgaaa aaatgacatg gaagaatgaa attaaaagta
    23041 ttcttgtaaa agaaaatatc agcatgaatg agttaaataa tttaatgaat gaacataata
    23101 ataagaataa tacagttgaa aatttaagac aaaaaataaa taatgagact atgaaatata
    23161 gcgaaatctt gactattgct aatttgatag gatatgaagt tgtttggaaa aagaaagaaa
    23221 agtagacata aaaaaaggta acaactaact agttgctacc ttctaatcta tctatcaata
    23281 aaatctaatg ctttgtaaag tgtttcaaac ctatcattac cttttatcat agtataattt
    23341 tctttagtca taggacttat cttttcacat gctccacccc ctacgacata aagattttgt
    23401 gtctggccag gtacataatc ttttatatca catattaata ttttcccatc attataaccc
    23461 catccaacaa cagttgcagg gattttgtca acttctccat cataaactat tgtatgtttg
    23521 tgcatctgtt taactccctc attatttata tttttattta atacaccttc tacaattaac
    23581 ttagcaatac cttcatgacc tagtttctta gctttctcat aatcttcttt gttatcgcaa
    23641 aagaaacttt caattagtat tgcagtaggt ttagaactat ttaaaatata taaactttta
    23701 tctaatttaa cacctctatt acctacttct ttatctcctt tttttcttat gaaaggttta
    23761 gataatttat ctactaccct ttgcgcatat tccttccctt tttcactata gtaaaatact
    23821 tctgttccaa aggctcctac accacttgaa tttaaatgca actctataag taagtcatat
    23881 cctccactat taactctagg tattttataa atcttttctt ctgctttagt tttaaactgc
    23941 ttttcagggc atattattac atctgcctta tgaccttctt ttctaaaagt atctgctaat
    24001 actggtgcaa gagatttatt gtattgatac tcgttaacta ctccattagc agaagtacat
    24061 gccccgcttt ttaaaatgct gtgtcctact gttatacata ttttcattat ttactacctc
    24121 ctttaacatt taattcatct gtcatagtat ctaataaact accgattttt tcttttaatc
    24181 gtttaggcac tggtaatcca cacaaataca tgttttttaa tatacttaca ctttcatata
    24241 gaataaataa aatagagaaa aattcagata ttccaagatg atttaatcgc aaaaaatcaa
    24301 cccaatcttg tggtaacata aataaaaagt taaactttgt tagaatgtca actactgcta
    24361 gaaaaaatac acatgctatc attgcaactt ttcttattcc tccatttatt ccaaagctcg
    24421 aattaaactg atgtgttttt atcgctctta agcatcctag cagtgtatca aatgctattg
    24481 ctaatattac taattttata aatatatttg ttgctaaaaa aactattgtt acgttcatat
    24541 ttcctcctta ttttgcatta aaataagact tagaaattat ctaagtctat aaaaaaaaga
    24601 acctactaca ctgttggttc tactggtgtt tcttctttat ttaataaacc tgttaactct
    24661 aaatattgtt cttctgtaat cctatttact gcatagaata catccatctt atgttgtaaa
    24721 tcctctttgg tactatagtt cttttgttct atcattaatt ttaataagtt atacatatta
    24781 attcctccta aatattgtta tttaatttca tattttctac ttcgaatgct gtgtttacta
    24841 tctcactatc tctattttta ttttcttctt ttaacatgct taattctttt tctaatgcct
    24901 gtaatctctt ttgttcatct gtcaaaacaa tctctatatt ttttatgttt agttcttttg
    24961 taactggatt tatagactct atatactgtt tactatagtc tatacttcca tattcaacat
    25021 ctaagtaatg taattctgtt attgtatcat gttctaatac gtcacctgtt gtttcacctg
    25081 tttggagtaa tattttccca tcttgattat aaattactct atttcctcta tccattctat
    25141 cacctcatat tattaattat ttaaaagctc tatgtctaac gcataccatc taaaagtgct
    25201 agtataaaaa ctaataggaa atttaattcc agcacttaaa tcactgatac ctatttcaat
    25261 attaaaacta tcggcagtaa tatctctaac attagcagtc ccaacaatac gagtatatga
    25321 accattatca tatttaagat ttcgacctct accactcata caaggataag cgtgaagata
    25381 cttaaaaggt tgagaagtat ctctaaaaga aatataacta attataatag tttttggtac
    25441 aaagtttaat ttagtaggaa caattaaaga attttgaaaa ccatctggtg gagtataagt
    25501 gccagtcgca tatttaattc gttgacttaa ttgattagtt aattctgtta tcctattttg
    25561 caactcctca acactagcat ctgaactatc aaaagaagtt ttaattttct ctgataactc
    25621 tactagcgta ttatttaaac ttgcttctat gttctttaat gctaaagtat ttataatact
    25681 tgttttgcca cttttgaatg tatctttaat ctctatccat ttgtttgtaa cttcatctgt
    25741 tgtagaacca gcaggaagag gtgcaattcc tttgcttata cttagtactt tttctgctgt
    25801 agcattatta ctgtctgtaa caactatttt aagtgtgtgt aatgcattat cttctaatgt
    25861 gtagttaatt attttttctg ttgttaaatc tgttgttata gtttctttta gtacatcatc
    25921 tataaagtat tctattttag ttagtagtgt agggtcagtg tggtcagctt tgaatgttgc
    25981 ttgtgtagaa ttataagatg atatatttaa aaatggtaat gattgaagta aagttatttg
    26041 tgcgtaacca taagcaccag cagtatttcc acccggtgtc ataacaatat tatcaaaata
    26101 atattcagat gttggtgtgt agccagtagg cttataactg tctttagtca atacataact
    26161 acttccacct ccacctgctc ctacaccatt catgcctgca ccaccaaacc agccacctcc
    26221 gccgccttcg ccagttgaat ttttaacaga acaccctttt ccaaaacttc cgttttctgt
    26281 atttacacga ccaataccac cttgatattg agtaccaccg ggacggtatc tgtcattaga
    26341 actataccca gtacctcctt ctaatcctcc tcctgcacca ccagtgtaag gatgatatga
    26401 acctccgcca ccacctgcga caattatacg agatagcaaa ccttgctcat tatcccaagc
    26461 accaccaaca agtcttatat cggtagcacc accaccgtac atagaataat aagtacccat
    26521 aacctgttga tttaaataac ctttaccacc gccattaaaa ccacttttag tgttattagc
    26581 tgtagatgaa gaagcaaaac cactttcgcc gacgtaaaca tataatgtag tttgtttttt
    26641 taatgtaatt tcacctttag aatatccgcc tttagcatca gtataccaag aagaattgtt
    26701 gatacctcca gaagaacccc agcattcaaa tttatattta ccaggtttca atataacact
    26761 ttgtggcgaa ccattgtacc caaagttcca ttcagtctgc attttctcac tctcctatct
    26821 taaataggta acatatcatt ttgtatagat atattaacct cattaaattt ttctattttt
    26881 ctaacaactt catctattgc cccttgaaca tttgtagcag taagattact tgttgcatta
    26941 ttatagcttg ttttctctgc tgttgtttct atgctatcta cactagcttt tacctcattt
    27001 aaggcactaa ctatatttgt tttatctgtt gtagtaagtt gtgttgtatc ccctattttc
    27061 ccatttaact ctgttttagc agtttctatg ttgcttgtta attctgtttt ggttgtatca
    27121 attttagcgt ctaaatcttg tatgtcttta agtgttgcta aaattacagt agggtctact
    27181 tttagattga tattagctac attagataca actaatataa cttttattaa gagttctttt
    27241 acagttcccg aatccgcagt aggtttatat gttgttgggt aactagaaat cgccaataat
    27301 tggtctttag agtcaaataa tccgacctct cttacttcaa atcctccaac atcaccaggt
    27361 ataaattttt gtattactac ccagttagga ttgtctttat ctgcttgtgc atgttcaagt
    27421 gtactttccc aaaccacatt ttttagtgct gtttgactct cagttggagt ataagagcta
    27481 cctccaccat ctccaacctt catttttgca aaatctactt tttcacctgt aatacttgca
    27541 tttgctatcg ctgctttacc aatttcagtc actaaagtaa aatattgttg ttctgccaac
    27601 tttatcacct catttctatt ttttagggta caatgttacc ttttctaatg ttctatcatt
    27661 tccactgcaa atagctattt ccccaaaact ttctaaatct ctaggtacat aaggatatac
    27721 tgtaactgtt tctcctgtgc taattgttgc gcctgtataa agcccatttt tgttaaatag
    27781 tatcctctcg aatttgtgtt caagatgcgc aggttttatt tcttctattt tcttatctaa
    27841 ttctaaaata gtgttataac tacaattatt tgttataaaa ctaagtgtaa aactaaatag
    27901 attgctaaaa acttctacat caacattggt ctttgtgtaa gcttctgata tagcttttat
    27961 aacttctatt gtagttgtgc ccttgcctct taatttagct cttatattac ttcttctagt
    28021 atcaaaatcc aacttataat ttatttttat acctaagaga ctttcccaat catcaagccc
    28081 ccaagttgct gtatctataa aaaactgatc tagtaaatca tctttttcgt caattaatgt
    28141 tagaagttca ttttcaagtg cttcctggat ttgtatgtca attttattgt ttgcaaaact
    28201 tggtagataa tcaattaact tcaatttaac tcacctcgac atcaaatgca acacttgaaa
    28261 cagcaggaac tttttcttca tcaactatta tgtttttaac atcatcattt acaaggagat
    28321 tttttatatc atgaacacct tctatgcttg ctaataaact cattactttt atataaataa
    28381 tctctcttga attttctatt aaataagaat ttataatatc taaaaacaca acttttatag
    28441 attcaatatc atatccattt tcaagtgtta aagttgcact aatattaata tcaaaagtac
    28501 ttggtgtaac aattgtaaca gtaggtccaa taggtttttc ttcttctata tgttcagtac
    28561 atctttgcaa tacttcatta tcaactgatt gattattttg accataaatt agaatcttaa
    28621 ttgtacctgg accatcccaa cgaggaatta cttttgcatt ataaacacct tcaacctcta
    28681 aagcccaaga ctggtaatgt gctttattcc cacttgttgc ttgatttttt tgtgttttat
    28741 agaacctttc ttttaattct tcatcagttt ctatttctgt tccacccttt aggtcaaatt
    28801 cgttataaat tttagttact ccagttattt cttctataag tttaaattct gtgttagctg
    28861 ataaattata cttaatgcca acctctaaag cctgtatagg gcttacattt aacttactat
    28921 tttcatctat tgtaatatct ttaataatta caaatggtaa atcactatga aatattattg
    28981 ttccatttgg tattttagtt cctattttac cctcaaatgt tacctcgcct attgcctctg
    29041 tgcctaactt tctatataca ccaaattcat tgactcttct atcaagaaaa tcgtcaaaat
    29101 tatcttgaat aaaaactctc ttatgtatat aagaaagttc tatatataat tgtgcaagtt
    29161 ctgaatttat tggagaaact atattataaa gagaagatcc ttggccttta taaagaggga
    29221 gatttatgtt gtttaaagtt ctattattta aggtactgaa tgattggcca ttatacaaaa
    29281 gtaatctcct cctctccata aattgttttt acatttaaac ttatagataa attatcatct
    29341 ttaaattctg tatttactac attaacttct aatatatatg gattagttaa tagtgcttct
    29401 tgtatatacc tttttgcctc actttcagta agacctttag tatacttttg ccctattaga
    29461 ttttttatat ctgttccata atcccaatca tatattaaat gaacatactt atctgttttt
    29521 attgttttgt aaatccatac tttaattgct tcatttcttt caactatttt aaagtcccca
    29581 ttttcaatta tcttttcatc tttatcaaaa tcccaagcaa attctttaaa aataggtaat
    29641 tcttcattgt ctggagaaat ataatcttct ggaacaccca taaaaggaaa tatagtatta
    29701 gtcatttaag accaccaact tacttataac agcaaattta tcatttattt taaacattat
    29761 taccatatct ccagcttcaa aagtatctat aaaaggattt tttatattat gtttgtgctc
    29821 tttatttatt tctgtattaa aagattctat ttgtctgtca agaatccaac tatctattaa
    29881 aatatcttct ttttccaata aaagattatt tatctctatc tttaaatctg gtaatttatc
    29941 tttaacttta ccaataaaaa aagaaggttc attatgaaat ttaccttctt gtctcattat
    30001 tcctataaat tcattaattg gatctgccat gctatcacct ctttttataa aactcttcta
    30061 gccgtattaa aatcttttct attacttaat ttacttattt tgactacatc tcctgtttgt
    30121 ggcgcatgta aaaattctcc attacctata tatagtccaa catggctgac tggattatgg
    30181 aaaaatacca aatctcctgc ctgtaaatta tttttctcta cttttttacc gactttagat
    30241 tgctgacttg aagttcttgg taaattaaca ttaacttttt taaaacaata taccataagt
    30301 ccagaacagt caaaattact tggtccattc ccaccccatt tgtatggctt tccaaggtgt
    30361 tttcttgctt cagctataac agtttttgct ctttctgtca tgtttccaga ataaccacca
    30421 attataatct tcccttttct tcttccaaag ttattggctt cttcaacatt tccaaataaa
    30481 atatctatat gatatgttcc atccttttct ataattattg caggcccatt atcatttacc
    30541 ttatacgttc catctcgact tgaaacacct gtaaccaatt gtatttcatc tccatatctc
    30601 ataagaggat gtttgtttaa gaaatctttt gtatagtaag atttttcata tgttccaacc
    30661 ataggagcag cacatgtttt tttagaagga tctagttttt tacctctaca atctttatct
    30721 ccaccctcag attttctagg acaataagca gtaaattcag cagagtattc tgttcctcca
    30781 gtatactctg aaccatcttc tttttgttca tcttgaccag cagatttttc atccattata
    30841 ttctcaaaat taagctctag ttcaatttga taatctccat tttgccatgt atgcttgtct
    30901 gtatctatat aaaaaagacc tattaatttt gtatatgagt cttttacttt tacacctcta
    30961 ccagttacac aagttatatc accatatcct tttaaagagc aagttttttc gattccttta
    31021 aactctccat ctatgtctat agtactgttt tcttgttgtt gtattacctt ttgcataatt
    31081 accccaacgt ctttaaatat agagtcattt attttctcac taattttatt accatactga
    31141 tcaacaacta gtactttatt ttttacattc ttaatgcttt cagaaaaatt tgtattgata
    31201 agattaaagc cttcttcaaa tgttatattt aaagtaacta cacccttctc aatgacatta
    31261 aatttatcaa gattagactc tatcatatac ttttttttag ttgacttgct tgcttctgta
    31321 taaacactca ttatagtatc ataaccagtt acgccaataa acatttttgt atatttaact
    31381 ccagtcttag gcaaattacc tagtgggagt ttattttcta aaaatacttg ttttgcaata
    31441 tcttcaacta atttatcttt aaaattgtat gacacttcgc tttgtaaaag taaaaaaccc
    31501 atatcttttg cagtgaaatc aatcatatta ttacttgagt ctaaagatct atctattatc
    31561 attcctctaa aaagttcttt atcatctaca tagaaacaaa ctgtacttgc tactggaata
    31621 tctatctgcc taaaattaat atcacttgca gattgaatta tagaaaattc caaagttctt
    31681 gatggtgatc tataatcacc agaccaagtg catttatcca ctaaatcagt tacattatat
    31741 atatttccgt tttttatatg aacttgtatt tttattttat taattataaa tcaccaccat
    31801 tttaaggaat tattaatacc caaccatttt ttattacaga tgggttttta attttatctt
    31861 catttgcttt ataaatcttc tcccataaat ctccattgcc ataatatttt ttagctatct
    31921 taaaaagagt atctccttca acaaccttat gtgttttttg cttagtatca aatccttttg
    31981 ttagaggaac attcttttca gaagataatt tttcatcatt attaatgttt actttggata
    32041 tttgtatcct tctgtattct tttagactta aggtaaaata aacatctctt gaataatctt
    32101 gttctctata attaaaatct gcaattatac attcaaagtt tatattagtt tctgtaatga
    32161 taaatcttaa tatatatcct tctttcatcc aactttctat taaatttaca caatcatatg
    32221 gttttggaaa tccattataa ttacagaaac tatattcttg attagggaaa aaagaggata
    32281 gttctatagt tttaagccct aatcctccaa atactgcgat atctccaaca tttaaaatat
    32341 tagatgtatt tattgtcgca cttccattta tctcaaaaga gggtggaata acaggaaacc
    32401 taaatgtatt atttgcttgt cttaaccata tttccattaa actcctccta aaataaaaga
    32461 cacttattga aataagtgtc ttttaagata attattataa atcaaatact gtatattttc
    32521 ctacattttc ataagcaatt tcaattgttt ttggctttgt atcttttgta agttcaaatg
    32581 ctatctcacc ttttaatgta tctcctgtat tggtgatttc aatctcaact gcatatacat
    32641 tactatctga ttctagtttt ctaacatttt taacagatac ctttgctccg tccaaatcaa
    32701 caacttcgcc tattttatga tttttaattt cctctgtatt ttgcttaggt ttactatctc
    32761 cattttttga atcatttgtg ctgcatccaa ctaaacttaa agataataca attgatattg
    32821 ctaatgctgg tattttttta ttcataacat aaccccctgt aaattatata ctacaattat
    32881 agcacgataa tttacaaaac gcctccaact tttgcaagag atattttttt gtttattttt
    32941 cttacaattt tatctatgtc agcctcttct cttacaatta ttgtatcagc caatttatca
    33001 agaaataaac ttccatttga actatttctt ttgtattgat tggcttcttg tttggttaaa
    33061 agtttctctc cttcatgtgc cctaattaaa taatcatttc taggaactct atttatacca
    33121 aatgcttttc ttggactttt gccagcagca tctgcgcctt ttcctatttg taaactagga
    33181 ccacttttta ctatctcgat cattcctttg attggattat gaaaaaattc tttaagttta
    33241 ttccaggctt cttttacgcc tttaacttta tctttaaaaa gagaatcagc aagatctatt
    33301 acaggttcca gtacaccact aactagatca acaaggccat tccaaattga ttttattaca
    33361 cctactccac tatcaaatat ttgtttaagt ccatccatag tttgatcagc atttcctgtt
    33421 ataagcccca taataacatt aataattcca gatataaaag acataattcc atttatgatt
    33481 ccagatactg ttgttattat tgcggttaat gtatttaaaa cccatgtaaa tgctaaaatt
    33541 attccgccaa caacaccagc tattgcaatt cctatagatg gtaaaagttg ttgtcctatt
    33601 tgtgatagga aaggcataaa tccttgaaac caagctttaa caggagcaag tgcttgagtt
    33661 aaactttgga aagaaagttt aattccatca gtagactttt taacactatc cattggttgg
    33721 cttactttgt ttactgtatc tccaacctgt ttggctggag taaaaaaatc agtaatcgct
    33781 tttttaacct tatcaaaaca ctctttcaaa ttatctaaat gaggttttaa aggttcaaat
    33841 gcttttacta gattatctat atttgtttta atgtttccag ctaatatatc ctttaattcg
    33901 ttaaatttat cttttattga agttattgac tccttaaatt tattcttaaa atttactcca
    33961 gaatcctcta gaacctttag tgcaggcttg acatcattta atattttatt ctgtaactct
    34021 gtaaatttag attttccaga ttcaactcct tggcttatag aatctgtcat aggcttaata
    34081 aaagttttaa aatcactaaa tgaacctttt agatttgcta ctgtttgttt taatcctcct
    34141 gtgtttggag ttgattttcc agctgcatct gctgctttac ttcctggaat atttacattt
    34201 gttttggcgg atattttggc agttgcttca attggatttt ctaagaatgt tcttaaatct
    34261 ctccacttat ctgttagcca tttagcttta tccttaaata aactgtcagc tatatcagct
    34321 atagcttgta taggagcagt tacaaaatct atgaatcctt tccaaattga ttttataatt
    34381 ttagttccac catcaaatat ttgcttaact ccattcatca tctttttact atcgcctgtg
    34441 acaatgccta taattaaatc aaatattcca tttaaaatag atgcaaggcc atttaataca
    34501 ttagttatag tatcaactac agctttgaat gcagttgcta gagtagataa aactacaact
    34561 aattttgcta tatgaaaacc tattacacca attaaaacag gtcctagtac cttcattatt
    34621 acacttccta agtttaagat agatgcaaat agtggaccaa atgctgataa caactcttta
    34681 aatttctctc ctaattgttt aagtgcaggc atacaaaaat ttactatagg ctttgcgaat
    34741 tctgtaaatg attttactaa tgaattaact ttatttctaa atacttcaga tttagcataa
    34801 gcaacagtaa aggctactga taatgctatt acagctgcta tagtccaacc cataggaccc
    34861 attacagcta aaaatactgc acttaatggt ttcattccaa ttacaaaggc tctaaatgca
    34921 acttttgttc ttaatattat aggtataaag aaagcgaata agcctgctgc tttagaaata
    34981 acaagactta caatccctac agtagccaac aacaagccaa atgttgtaac agctaacaat
    35041 atacctgtta cagttgattg gaaggctgga ggcaagttag agaaagcatt agccatatta
    35101 gatacaactt ggactgcact tcttataaaa ggtaataaat tattccctat aattattgct
    35161 acttgagtaa tctgattttt catcatctta agctgcgatt ctgtcgttga atatctttga
    35221 tttgcttcat ttgttagtgc tacattctct ttccaagcgt catttccaag ttgaatagcg
    35281 ttgttaaata gatcacctgc tccacttgct ctaagcaatg catcagtcat gcctatttca
    35341 gttattccca tcttatttaa catttttata gcactatttc catgtttttt tgcatctcca
    35401 agtcctttga caaatgatat aattgctcca gcagcatctt tttgaaacga ctctttaaat
    35461 tttgatgcac tcatgccagc aacttttgca aagttattta atttacttcc tcccatttcg
    35521 catgataatt gcatttgttg cataacctta gaaaaagcag aacctcctgc ctctgctttt
    35581 ataccaactg aacttaatgc accagcgaaa gacattattt gcgcctcaga catcccaact
    35641 tgttttccag aaccagcaag tctaagagcc atttcaacaa tttcgctttc tgtagttgct
    35701 aggttatttc caagttgaac tatcgttgct ccaagtctat caaagttttt ttgtggcatt
    35761 tgaacaatgt ttgcaaatct agctaatgct gtagctgctt catcagacga catgtttgtt
    35821 gcatctccaa gcataaccat tgtttttgta aaatttgtaa tattagatgc atttttgccg
    35881 actttaatac ctaattgtcc tgctgcttct ccgacagcac taatctcaac tgccgtttgt
    35941 ggcatatttt tagataaatc catgatattt ttcttaaacg tattaatatc tgcatcactt
    36001 gcatttacag tctttttaac tcctgcaaaa gcactttcga attctattgc agattttgca
    36061 cttaaagcga gtatcccacc agacacagca gctatagcac tacctgtttt taaagcggta
    36121 gaaccaacat tttttaaatt tttttggaca ttatttaaag ccttagaggc gttagaagtt
    36181 gagtcatgca gagatcttgt atttgccgca actcttctta aagttgcact agcttcatct
    36241 ctcattctta taattgcttg taaaactctt gtaccatcac tcatgtatta aaaccacctt
    36301 ccagcgccag ccttcatttc ttcttttctc tcatccaatt cttgatccat gaagatacta
    36361 gtaatttctt tttcgccttt taacatgcga taagaaacgg atggcattat acctttatac
    36421 ttaaataata agtacattaa gttaacttct ccatccgttt taattagttt tttatatctt
    36481 tagctctttc tttttgtctt tttttatcgc cctcagcatc tatgccattt actttattaa
    36541 cttcctcata taatttatct atttccccac taagtagtag ctttttaacc aattctttag
    36601 gtgtaattgc gctaaatttt ttaactaatt ccatattact aaatattggg catgattcta
    36661 atattgtatt tactttaagt tcatatatgt taatgttttc tacatctccg ctattaacat
    36721 ccatcgagtt cattcttatt tcgtcaaaac gttctggatc aatagcctta cactctatct
    36781 ctaaagtatc atctagtttt ttacaatata attcaaatgt gctagatggc atttttaatt
    36841 tacctgcatc catatttaat aatttttcta ctgtgttcat ataataaaaa tcctcctatt
    36901 tttcttttat tttatctaag tattcaaatc cttcaaatgt gaatggtact tctatttctc
    36961 caactttcgc agcttcccaa tcagccaaag ttaagtcgtc aaacatacat ccataaagtg
    37021 caattctctc agaaccatat gaatctgggt cttgcaattt acttattata gttactttaa
    37081 attctttacc ttcatttaac attttttcga catactcgcc aaataaggta cttatcttat
    37141 aaagagttat agatcctttt ccagttgcac caatcatttt atatgtggtc attatttgac
    37201 cacataattt tatttcttct ttatctatat caaccttggc ttccatccct tttgttgatg
    37261 taagttgctt attatctagc caacactctc caaaagtacc agatattatt ctttttgcat
    37321 ctattttagc tttcaaattt taacctccaa actacataga gatgtctaag tatacatctt
    37381 ccattgcatc tattaattta attcttgctt ttaaaaatac aagagtgtca gtattgtatt
    37441 cttttatctg ttgttcttcc atattagata tatctatatt ttgttttttt agccatgctc
    37501 tttgagcatc taaattaatt tctacagttg aaccttcatc aattaaacca tctttttcta
    37561 attcttgaag atataattgt attgcaacaa taagtaaaca cttattatca taagtgtttt
    37621 gagtttttcc tatataattt tttacaatta tttttttaat atcgttatga atttgatcta
    37681 gtgtatcaac caacttaatc tttttgaata tatcaccttt tgaatcagta attgttgtaa
    37741 aggatgtaac tcctctagcg attactatag ctccactttc ttttattaaa attagctctc
    37801 cattatttat ccttgtattt gcttcagctc ttgttagctt aggtatatta gtaacttctg
    37861 gtatttctgc atatgttaca gattgagttg atggagttcc tgcaataaaa cctgctattc
    37921 ttggcagaaa ttcatttgaa gtgtattttt taccttcaac ttcaatatca gttgctgtaa
    37981 agtttattat accttcataa tccgctgaat tacttgcagt aatagcttta acctttattt
    38041 tattatcttc tctcattttc ttaatccacg ttttgatttt aggcaaatct tgttctgtct
    38101 catctggcat acaaagataa ttaaactcac aagtttcaag aaaatctagt gcatcatcta
    38161 ttgattcatc tgtatctaat gtataaacta atactttatt tggaggatat acatttccct
    38221 tcatagccat ttttatataa tctaaattag tgggagaata attttctgga atatcctcta
    38281 tttcttttat ctcaactaac cctatatttt ttgtatcttt taaaataagc gctacaatac
    38341 caccagagcg tttaacaaaa gtttttcctg cttggataaa agaaatatta atttctggta
    38401 aacccattta atcacttcct atctctaaat ctacactttc cataatttca aatgtttcat
    38461 tttcaaaata gacttcttca aaataagata cagtcattag aaaagttagt ttatatccta
    38521 caggatcttt tgcgattgat ggagatatat tctcaatgat taaacttcta tctttaactc
    38581 taatgtttct gttaaatatt ttttctaatc tactttgtat gtcgtataga tttaatttat
    38641 tttttctagc cttttggtaa tattcaatat ctatgaatat ctttttagta tctaaaactt
    38701 tatttgcaat attattagaa acaggtaaaa gttgcacaaa aaaacaagcc tcctcaaagc
    38761 cttgtatatt atcctcatta ataaaaatat ctatatatct aaactcattt tcgattcttt
    38821 tattaactgc tattataatg tctctgtttg taagcaacta atcacctacc aatcaaataa
    38881 atttgctttt gcacttgtca tattttcttt tgttctttca attccctttt caaccataaa
    38941 agaaccaggg acaatgccac catttttagt tgaatggcca ttatttacta aagtagcata
    39001 atcaacttca tttttaacaa tataagtatc atattctggt tgcttttctt cccatccctt
    39061 ttttaacttt tcagttttta caggagtttc atctcttata ttttctgcta gttcttgccc
    39121 agtttctttt gtaaacttct tgatttttct attaaagttt gatgcattta gacctaaatc
    39181 taaggctaat gcttcaaaga ctcctaaatt atctattccc attatatcct ctccaattct
    39241 gtaataggta tttccatatg gcttggatat atatttgcct tagaaacctt aaatacagct
    39301 tcttctccat gattataggt tgtatttata gtatcaccta tttttatatc tacttctggt
    39361 ctacaaaata actttctatt tacaactata gatccaatat caccatttac aatggcttct
    39421 tcttttctag aaattgcaca tgggatattt tcagctataa caacattact aaaatcatta
    39481 gaacatgttt cttcattcca gccttttaat tttctaatta tagtcatttt atcaaggtat
    39541 gtcatttcta atatatctgc ttcagtcatt taatcacccc ttagtacata ctaacaatac
    39601 aaaatgggct tagaaatttt ttatcagtat cagttaaaaa agatgatgaa ctgatgctat
    39661 tttcagcagt ttgtaatgtt aaagcattat attctatctt agtgtcacct ctagttatag
    39721 cttttaagct tttttctaga ttatcaagtc cactattttt cttaagttct tcaaatcttg
    39781 aagataatat tgatattact ttttcttcta caaacccttc tagtgctata cttagttcct
    39841 gcctagaaca atattctaaa accatctcac ttattttatt tatataaagg attattaatt
    39901 tgtcatatct atcattttct attcctaaaa ctaatttaac attatcaagc attgtactca
    39961 tctagtagtt gaattaattc ctctttagtt ttagttgaat atccctttat attcttgcct
    40021 ttagcaattt ccataagctc tggtttagat agattaaata aatcattaga ggttgattta
    40081 actttttctt cttttttctc aatattctct ttagtcactt cttctgaata tattttaaat
    40141 ccttttgaaa tcaatttatc tctcgaattt tcatcttcaa caactttaat tatgttattt
    40201 tttatcaatg caaacattta aaacaactcc ttattcttgt gcagctttag cttgtctcac
    40261 agatgctcta caaagtttta aatattcatc caatatccac aaatcgtgat attttctata
    40321 gtctatttga taagcatctg ctgtttggtt ttgatctgga gtaaatattc ttggcttatc
    40381 agtcttagat actgcaattg gagcagatgc tggagttatt aaccagttta tatctaaagc
    40441 ctttgctcct tcagtaaacc caccctctgt ttgtccagat gttttaccat ctctaaatac
    40501 gtattctgtt ttcattcttt ttgatggaac ttttattata ggtacattgt ctatagactt
    40561 tacaattctt tttatatctc catcgccaaa ttcaatatca tcaagttgag atgattgttc
    40621 taatatgctt gcaattatgc tagacatact aataataaga ggtatatctc cagataaatc
    40681 ttgtattgat gcaagttgat ttttcaattc acttaatatt gtagttactg tagcttcgta
    40741 tgaatatttt acacgctcat ctactttagc tgtttcagaa aatatctttg agtatctata
    40801 tgcatctatt tctggtataa catgttgctt ttgaaataat cccattacag aagatgcatt
    40861 tataacgaag ttactttcat ttacatccat tgaatctaac ataaaagtcc tacctctatc
    40921 catctccatt ttcattgttt cgtactcaag tgtaactgcg ccttgcgtat atcctttaga
    40981 tctgtcataa tcacctaagc catccattgt tacttttggt acttttacct ctcttcctcc
    41041 attatatata acttgaccag catttttctc catccatcca gtagttgagc caactgttac
    41101 ctgcatatct agttcttgtt gaaaaagtgt tacatattgt aatacattag ccatttataa
    41161 aatcatcctt tcttttttta tactaaagac tatttagccc ttgctttatt tgttttctaa
    41221 tttctgatag cgaacctgca ctatctttgc caggtggaac gtatgaacca tctttaattc
    41281 tttcatctac ctttgactgc acatattgct tcattgaatt ttcaaatatc tctatatttg
    41341 catttgtttt atcatcatca tcagataata gatattcaat taagtttgta ggtattttct
    41401 tttcaactaa aatatcttta tatttagaaa ctttttctgc gtactgcttt tcttttttta
    41461 gtttgtctag gctttctctt aattccctta ttgctttttg ctcttctgtt tcatttgctc
    41521 cagttctttt tagaacttca gcacttataa gtgtctgcat atcttttttc ttaaagtttt
    41581 caagtgcttc gctgtgatat ttattttttt cattatctat aatagcttta aagtttgcat
    41641 cattattaat taattctcta aactcttcta ttgtaagctt tttatcagat gcagatttga
    41701 ataaatctgt tgattgcagc aatgaatcta tttctgcatc atcttctatt tcttttagta
    41761 attccaacac ttcttttttt aacatgtata aagttctcct ttttgaaaat ttaattcaaa
    41821 cattctattt tatccataac ctttactatt ttaggaaatt gaattgcaaa gaagtcaatc
    41881 atttcttcgt ttgtagccca agaactacaa ctcttcaatc cactttcact aagaaatgca
    41941 tgaattattt catgcctagc aacttcattt ttatatacac ttaaatcttt caatgaacct
    42001 tcttcagaat ttattttctc tataacaatt tccttaacag agtgatcaca atatccagct
    42061 ctttccttta atagcacatc ttcgctttca agtttctcta tcactagata ttctgttcct
    42121 aatatttcaa ctttcatatt tcacctcata ttttttagac ataataaaaa cacctattaa
    42181 attaattaat acatgttaaa caggtatata ttgacatcta cagtgacagt gtcttggcaa
    42241 agagggccta tcttcattga cataaaatat cactccatta tgcatagaac aatcactaca
    42301 tgtgcgagaa tctaaatctg cacaatacat tacttttata atcccttgaa ccttgaaaaa
    42361 atactcattt acttcacttt gacatctggc aacttcattt tctattagtc tgctggtttg
    42421 tcctgcacct gttgaatatt tatctcttat tgctttttct atcttattta cagaagtttt
    42481 tccatttaga aaactcttta tttctttttt taaggactct tctatacctt ttttattctt
    42541 ccagagtcta tcgctccaat gttttccatc aaccttatta ttcactatct tatttataac
    42601 tttattgttt atattactaa catcttttat cttcagtatt actcctaata aaattatcaa
    42661 atttgaatat ttattcttag atgaatcaga taaaatagtt tctatatcct tgacttcttc
    42721 atcataacta ttagaatact catttataat tttattaagt tttgacctta gatttttctt
    42781 atctttatta ttaatattta agtattcatt aataatatcg tgtgttagca atatctttgc
    42841 tatttctaat aaaagtttat ccctattttc tctttgatac ttataaaatg gttttgtcct
    42901 attttcagca gatttatata tatcttcagt caatttagtt ataatctttt gattcatcta
    42961 ataaaatctc tccctctgaa actatttcct gttctttttt agctttttct atttcagcat
    43021 gaggattatc aatgaaactt agttgagata agccagtttc agtagataat ttatctccaa
    43081 gttggcttaa tatatttgct acaactaaat catcacgtgg tatcttaggg gtaaatttag
    43141 ccttaatatc tttataatca tagtcaaaac catatttttt attaagccaa atgcacaata
    43201 atttgtacct attaaataaa gtatctgcaa tactatcatt attttggcta caaatccatt
    43261 ctgtactgat taatcttgct ataactgcta gacttgatgt attactttgc attttttcat
    43321 tgtgatttat atgagatgta atttcataca tattttcttt tattgaagat agtgtatttt
    43381 gaacaaatgt atcattcata tctttaataa gccactctat ttttccatct ccattaactt
    43441 gaagtattcc aagttctttc atccttggca aatcatcttc tttaatatta catccagaaa
    43501 aggttaaata agcatttcta aaatcactaa tttcattact tatatcactt aaattagttt
    43561 cataagcatc ttgtaaccct tttatatcat taaatattgt attttttcct agttcttcac
    43621 ttcttttgca aattgatatt ggaacatttc catcaaatat attgtttact gtaggacttt
    43681 caacttcttt gaaatttgaa tcaaaatgat atatatattc tttgtcatat acatctacat
    43741 acgtgtcatc ttcgaaacct ttggtaaact ctctaataaa gcatactatt tctccatttt
    43801 cattttcata atgacagcca tctgttggct tgattatctt tacttgtatt tcatcatctt
    43861 ttacatagta aagttcatag caaagcccaa ataatagtgc atacctcaat aaatctgagt
    43921 catgtttttt actccaatgg caagtatagt attctaaatc atttataatc ttctcatcac
    43981 cagatctact tgtataggta atcttatttg caagagaata agcgacttct tcattaataa
    44041 acttttttaa gaaattagtg tttatcttat tattagatct cttggtaacc attttatagt
    44101 tgctaatagc atccgtttca cctttataat acttatacat agtatcatat tcggatttat
    44161 tagagttcca atcgtcatat atgtctttta ttaagtctaa atctaattcc attaacaaca
    44221 catcctatcc tgtagactct ataaatttta aaacttttat tggaatatct tgctttgaaa
    44281 tataactttt actccttgct ctatgagtat atgcaaaata tcctttttca tcttttccta
    44341 ttccaacatt gaacttcttt ccttttttag ttaaagtttt ttgaacttct tcaaactttg
    44401 atctattagc caataaatta cctcccaact aaaaacctaa taattttcta tcaaagatat
    44461 ttactgtctc agaaactttt atgttgttaa ttcgattaat aaattccgat gatatatccg
    44521 gagcatcatc atgtagagag tatttttgtc ctgtaaaatc aagtatttgc tgtataaatt
    44581 ccttatcttc ttctgcaaaa atcatttggc ctttatttaa aacaggtatt aaagtagaga
    44641 ttttatcatc tttatttttc ttttgttgct cattaatgat ttctatatct ctataactaa
    44701 gaacattatc attttttatt ttaaactcta attggttggc atctgcgcca ttaaatgtat
    44761 ttttttctat ataaatatgt gttatatctg aatacacttt taataaataa atcatatggt
    44821 ctatatattt atcaaaatca gttctagcat ttattttagc tagttctgcc aatcttccat
    44881 atagtaaacc attttctgat tcacttccaa caagaaaagc gctatagtca tgtttcgacc
    44941 cacccgcaga tgctggatca cataacagca ttgtcttttt aaaagtatgc gattcaattt
    45001 cttctctagt ttctgttcta acagttttaa accatttttc tcctatagaa ttgacatcat
    45061 tttgcacttc ctgcttaaaa gatgttggat tttcataata acttagagcc atatctaaac
    45121 aatcccaaaa acttggccat aatataggga atttcatttc tttttcattt tcccagtaaa
    45181 attctttagc atcttcaaga tgatttgtat ttttaaaatc aaataagatc tctttgaact
    45241 taagccataa accagaatta aataattcat ctacattatc aactaaaact cctttttcgc
    45301 atttaaactt ccaagttggt aaatttttta gtctactata aaaacattct ttgtgttgta
    45361 aggttcctaa tgctataaaa gttgttcctc tttttatagt tttaccattt cttatgactg
    45421 gtttttgagc agcatatttt acatcatcag aaaatctttt aaattttttc tctctagcat
    45481 cttctgttct aacattttct tctgactgat aatcatcaag tatgataaga tctggacggt
    45541 tattattgta ttttcttcct ctcatgggtg atgaagatga tatagcttca ataaaagttt
    45601 tatttgttag ctctagttga gtactattac aaatgtattt tctatcatta tcatttaaaa
    45661 ggacaccaaa tgctttttta atatactcat tttcaagtaa tgcatttttt atatctttta
    45721 caaatttctc tgctgtagat cctatgtcag agcaaatcaa agtatatgtc ttatgtttat
    45781 agcaatgcga ccatacagtt gtcgctaaat ctccaaatgc acttttacct gtccctcttg
    45841 gataaattct tcctaattgc tgtgaaccat ctccaattat agattcttgt atatcttccc
    45901 atagctcatg atgaacttta gctataggag cagcagcatt atcttctttt ggtaaatatg
    45961 tgtcttgcat aaaatacata cagaaaaatt ctaggtttat ttttccaagt tgataagcga
    46021 gaccattaac tccccaaaga ttgtttgaat tggcttttat aatctcattt gcttcttttt
    46081 cagcatcttt tatagatgct ccatttctta taaaagtctt tgctagataa ctaaatatta
    46141 attgtatatt tctttctaga ttatccactc gtatttactt cctcttactt cttctatttt
    46201 ttaggctcag tgagtaatgc aattaataaa ataaacaatg taaatttttc tctaataaaa
    46261 tcaccctctt tgaaatacat ctctggtttt taaattttgc tagaaaattt ctagaactca
    46321 ttttccataa aaaataattt tttcaaaata gaaggacacc ccctattaat
//
LOCUS       DAKBNN010000008        45816 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000008 DAKBNN010000000
VERSION     DAKBNN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.008"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            122..238
                     /gene="rrf"
                     /locus_tag="OUB02_000470"
     rRNA            122..238
                     /gene="rrf"
                     /locus_tag="OUB02_000470"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            246..320
                     /locus_tag="OUB02_000471"
     tRNA            246..320
                     /locus_tag="OUB02_000471"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Asn,seq:gtt)
     gene            326..411
                     /locus_tag="OUB02_000472"
     tRNA            326..411
                     /locus_tag="OUB02_000472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:360..362,aa:Leu,seq:taa)
     gene            427..502
                     /locus_tag="OUB02_000473"
     tRNA            427..502
                     /locus_tag="OUB02_000473"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Met,seq:cat)
     gene            510..584
                     /locus_tag="OUB02_000474"
     tRNA            510..584
                     /locus_tag="OUB02_000474"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:543..545,aa:Glu,seq:ttc)
     gene            599..672
                     /locus_tag="OUB02_000475"
     tRNA            599..672
                     /locus_tag="OUB02_000475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:631..633,aa:Gly,seq:tcc)
     gene            678..753
                     /locus_tag="OUB02_000476"
     tRNA            678..753
                     /locus_tag="OUB02_000476"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Val,seq:tac)
     gene            759..835
                     /locus_tag="OUB02_000477"
     tRNA            759..835
                     /locus_tag="OUB02_000477"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:793..795,aa:Asp,seq:gtc)
     gene            846..920
                     /locus_tag="OUB02_000478"
     tRNA            846..920
                     /locus_tag="OUB02_000478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:878..880,aa:Gly,seq:gcc)
     gene            930..1003
                     /locus_tag="OUB02_000479"
     tRNA            930..1003
                     /locus_tag="OUB02_000479"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:964..966,aa:Arg,seq:tct)
     gene            1987..2826
                     /locus_tag="OUB02_000480"
     CDS             1987..2826
                     /locus_tag="OUB02_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCT8884279.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2823..4004
                     /locus_tag="OUB02_000481"
     CDS             2823..4004
                     /locus_tag="OUB02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884280.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4308..5210
                     /gene="ptb"
                     /locus_tag="OUB02_000482"
     CDS             4308..5210
                     /gene="ptb"
                     /locus_tag="OUB02_000482"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HCT8884281.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5232..6311
                     /gene="buk"
                     /locus_tag="OUB02_000483"
     CDS             5232..6311
                     /gene="buk"
                     /locus_tag="OUB02_000483"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HCT8884282.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6301..6978
                     /locus_tag="OUB02_000484"
     CDS             6301..6978
                     /locus_tag="OUB02_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8884283.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            7021..7236
                     /locus_tag="OUB02_000485"
     CDS             7021..7236
                     /locus_tag="OUB02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8884284.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7265..8344
                     /locus_tag="OUB02_000486"
     CDS             7265..8344
                     /locus_tag="OUB02_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HCT8884285.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8344..9096
                     /locus_tag="OUB02_000487"
     CDS             8344..9096
                     /locus_tag="OUB02_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HCT8884286.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9097..9654
                     /locus_tag="OUB02_000488"
     CDS             9097..9654
                     /locus_tag="OUB02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HCT8884287.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9824..11170
                     /locus_tag="OUB02_000489"
     CDS             9824..11170
                     /locus_tag="OUB02_000489"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCT8884288.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSLPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATDLANLIQEKLS"
     gene            11437..13269
                     /gene="glmS"
                     /locus_tag="OUB02_000490"
     CDS             11437..13269
                     /gene="glmS"
                     /locus_tag="OUB02_000490"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCT8884289.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13721..15016
                     /locus_tag="OUB02_000491"
     CDS             13721..15016
                     /locus_tag="OUB02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HCT8884290.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVRRGGHIKGAVDFSANWLSVYLDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15472..16209
                     /locus_tag="OUB02_000492"
     CDS             15472..16209
                     /locus_tag="OUB02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HCT8884291.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            16230..17483
                     /gene="murA"
                     /locus_tag="OUB02_000493"
     CDS             16230..17483
                     /gene="murA"
                     /locus_tag="OUB02_000493"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCT8884292.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17570..18634
                     /gene="spoIID"
                     /locus_tag="OUB02_000494"
     CDS             17570..18634
                     /gene="spoIID"
                     /locus_tag="OUB02_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HCT8884293.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18700..19368
                     /locus_tag="OUB02_000495"
     CDS             18700..19368
                     /locus_tag="OUB02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCT8884294.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19466..19726
                     /gene="spoIIID"
                     /locus_tag="OUB02_000496"
     CDS             19466..19726
                     /gene="spoIIID"
                     /locus_tag="OUB02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HCT8884295.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKENKKM"
     gene            19923..20942
                     /locus_tag="OUB02_000497"
     CDS             19923..20942
                     /locus_tag="OUB02_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HCT8884296.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20958..21386
                     /gene="fabZ"
                     /locus_tag="OUB02_000498"
     CDS             20958..21386
                     /gene="fabZ"
                     /locus_tag="OUB02_000498"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCT8884297.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21497..22006)
                     /gene="yyaC"
                     /locus_tag="OUB02_000499"
     CDS             complement(21497..22006)
                     /gene="yyaC"
                     /locus_tag="OUB02_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HCT8884298.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22195..22298
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22435..23628
                     /locus_tag="OUB02_000500"
     CDS             22435..23628
                     /locus_tag="OUB02_000500"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCT8884299.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23944..25041
                     /locus_tag="OUB02_000501"
     CDS             23944..25041
                     /locus_tag="OUB02_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884300.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25305..27524
                     /locus_tag="OUB02_000502"
     CDS             25305..27524
                     /locus_tag="OUB02_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCT8884301.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEILKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINTIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVEKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27527..28309
                     /locus_tag="OUB02_000503"
     CDS             27527..28309
                     /locus_tag="OUB02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCT8884302.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28641..30554
                     /locus_tag="OUB02_000504"
     CDS             28641..30554
                     /locus_tag="OUB02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8884303.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30695..31003
                     /locus_tag="OUB02_000505"
     CDS             30695..31003
                     /locus_tag="OUB02_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT8884304.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            31026..31352
                     /locus_tag="OUB02_000506"
     CDS             31026..31352
                     /locus_tag="OUB02_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884305.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31391..32725
                     /gene="celB"
                     /locus_tag="OUB02_000507"
     CDS             31391..32725
                     /gene="celB"
                     /locus_tag="OUB02_000507"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCT8884306.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            32736..33254
                     /locus_tag="OUB02_000508"
     CDS             32736..33254
                     /locus_tag="OUB02_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HCT8884307.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33350..34309
                     /locus_tag="OUB02_000509"
     CDS             33350..34309
                     /locus_tag="OUB02_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HCT8884308.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34358..35410
                     /locus_tag="OUB02_000510"
     CDS             34358..35410
                     /locus_tag="OUB02_000510"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCT8884309.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35413..36087
                     /locus_tag="OUB02_000511"
     CDS             35413..36087
                     /locus_tag="OUB02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCT8884310.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36640..37191
                     /gene="raiA"
                     /locus_tag="OUB02_000512"
     CDS             36640..37191
                     /gene="raiA"
                     /locus_tag="OUB02_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCT8884311.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37323..39998
                     /gene="secA"
                     /locus_tag="OUB02_000513"
     CDS             37323..39998
                     /gene="secA"
                     /locus_tag="OUB02_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCT8884312.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            40013..41120
                     /gene="prfB"
                     /locus_tag="OUB02_000514"
     CDS             join(40013..40087,40089..41120)
                     /gene="prfB"
                     /locus_tag="OUB02_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCT8884313.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41264..43405
                     /locus_tag="OUB02_000515"
     CDS             41264..43405
                     /locus_tag="OUB02_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCT8884314.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIESKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43553..44719
                     /locus_tag="OUB02_000516"
     CDS             43553..44719
                     /locus_tag="OUB02_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8884315.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44779..44896
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45021..45626
                     /locus_tag="OUB02_000517"
     CDS             45021..45626
                     /locus_tag="OUB02_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT8884316.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT        18413 a         5004 c         8775 g        13624 t
ORIGIN      
        1 tttgatgttc attcattaag atatatgtgt agtttttaga gtgctaactc taaagaaact
       61 agtattgcta ggttctataa acttattaaa aacttaatag aaatattaag cataaagatt
      121 atgtggttac aatagcagag aggatacacc tgttcccatt ccgaacacag aagttaagct
      181 ctctagcgct gatggtactt ggtgggaaac tgcctgggag agtaggacgt agccacgtta
      241 aatgttccaa ggtagctcaa cggtggagca accggctgtt aaccggtagg ctgtgggttc
      301 gagccccacc cttggagcca aaaaagccga agtggcggaa ctggcagacg cacaggactt
      361 aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc acttataaaa
      421 caatatcgcg gggtggagca gttggcagct cgtcgggctc ataacccgaa ggtcgtaggt
      481 tcaagtcctg cctccgcaac cattaaaacg gctcgttggt caagaggtta agacaccgcc
      541 ctttcacggc ggtaacaggg gttcaattcc cctacgagtc accaattcaa ttaaatatgc
      601 gggtgtagct caatggtaga gttctggcct tccaagccag ctgtgagggt tcgatcccct
      661 tcacccgctc caaaaatggg accatagctc agctgggaga gcacctgcct tacaagcagg
      721 gggtcacagg ttcgagccct gttggtccca ccattcgcgg cctggtagtt cagttggtta
      781 gaatgccagc ctgtcacgct ggaggtcgag ggttcgagcc ccttccaggt cgccaaatta
      841 aataagcggg aatagttcag tggtagagcg caaccttgcc aaggttgaag tcgcgagttc
      901 gaatctcgtt tcccgctcca taataacttg ggtgcatagc tcagctggat agagcaacgc
      961 ccttctaagg cgtgtgtccg gggttcgaat ccctgtgcgc tcattaagaa aagtttacta
     1021 taaattatag taaactttta tattgtttaa aaagacacag gaaacatgta tattacctgt
     1081 gtctttttat ttataaactt taaactatgt aaaaattaca gtatattcta atagagttac
     1141 agtatattca ttaatattgt aaacatacgg agggtaatgt ataatagtct acaagatgtt
     1201 tatgaaggca tcagttaaat ataataaata taactaatta gtaagagtta tatacattat
     1261 attgtatact ttatggtatt tatatattga aataacttat gaagttattt ataaagagat
     1321 tcatgagcat atttttgtct aaacaataag tataagaaat tataaaaaat tagagactat
     1381 agtgaattta caatttaatg taatatttat aagttatact aaataaatta ttatactgtg
     1441 aaatggatat ataactttag gtattgtgta ttattttcac tgtaggagat tatatctagt
     1501 tatacataat aagttttaat gatagtttat agtaagatat taaaataaat ttatctaatc
     1561 tagatatgaa ataagttatt actttaatat aaaaatgaat ctaatggaat aatattgatg
     1621 ataagtaata tttagatttt gtatttgttt atagttaata agtggattat agaatttata
     1681 ataattaata tatattaaac aaagatatat taagatattt tagaacacaa aactgcatta
     1741 aggcgcgagt actattttgt attcgcgcct tgatggtttg agttatttga gtaaaaacta
     1801 actttaataa gaaatctgta tttattaagt tgatattttt aatgtgttta gagattattt
     1861 aacaatctcc aaatataaaa ctagaattat ttacataaaa gttaaatagt ttgtatagag
     1921 ttagatttat atagtataat acttagtgta aataattaag aagttatcaa acaggaggat
     1981 aaaactgtgt tagatataaa ttctttttta aatggatttt ttaatataat attaagaatg
     2041 agtatatatg atttaattga catatctata gtagcgtata ttttttacaa aatatttatg
     2101 tttataaaag atacaagagc agaacaagtg tttaaaggta taatatttct tttattagca
     2161 actcaactta gtaacacctt taaattgcat accgtttatt ggatatctct taaagcttta
     2221 gattatggag ttatagctgc tcttataatt tttcaaccag aatttagagc aggacttgaa
     2281 catataggaa gagcaaagtt taatttattt ggaaagaatg taaatacttc tgaagaaact
     2341 ctcaatagaa atatagaaga aattgtagaa gctttatatt cactttctag acaaaaaata
     2401 ggagctctta tcataatgga gagagaaact agaataagtg atataataaa tacaggaact
     2461 attattgatg ctgaaatttc taggcaacta ttaataaata tatttatacc aaatacacca
     2521 ttacacgatg gagctgttgt aattagagat tctaaggtaa aagcagctgc atgttttttg
     2581 ccattgacag aaagtaagga tttaagtaaa gatttgggta ctagacatag agcaggaata
     2641 ggggtaagtg aggtgtctga ctgtataact ttgattgtat cagaggaaac aggtggcgta
     2701 tcaattgcta aagcaggtaa gttatataga gatatttcta gagaaagaat gatgaatata
     2761 ttacgtagta atttaaaaac aaatacagaa acaagaagtt ttttcaaagg tggtatattt
     2821 aaatgataaa tagactaaaa aataatacca aaataaaaat aatatcattt ttaagtgcaa
     2881 ttgcattatg gatgtatgtt atggctgtag ttgaccctga agaaactaaa gggtttgaag
     2941 atatacctgt atctataagt aatatgagtg atttaaagga taaaaatctt gttatatatc
     3001 cagacgaaaa attaacagca gatgtatata taaaaggtag tttatcaaag gtacaaagtg
     3061 ttaaaaaaga agatatacat gtatatggaa ccattgaaga cccaatacaa ggacagaagg
     3121 aagtatattt aaaagcaaat attcctcaag gtgttactct agaatttaag catgatgttt
     3181 tagtagtaaa tcttgaaaaa aacataaaag aaaaaagaga tataaaggta gaagtaagtg
     3241 gaaattcaaa aagaaatata aataaaattg aacttagtaa ggaatttgtc gatgtatcag
     3301 gtccaagaac tctagttaaa gaagtcaaaa gtgtaagagc taatctagat gttggtaatg
     3361 agacagatga tttttctaaa aaattgcaat taataccatt aaatagtgat ggaactaagg
     3421 ttgaaggagt aacattaagt tcatcctttg tgactgcaaa gatagaacta ttgaaggaaa
     3481 aaattgtccc aataagagta agtctaaatg ataataaaga taatgatgta ctgaaaaact
     3541 ataaattaag taaggaacaa gttacaataa aaggaaagaa agaagatata gacaaaataa
     3601 gttatattaa gacaaaacct attgacttag ctgatttggc taatggtgaa gaaaaagaag
     3661 ttagcttaga aattccagag ggtataactc ttgaggatag ctctataaca attaaattaa
     3721 atgatacaaa acaattgagt gcagaatttt tatataatgc gggagatatt gaattaagaa
     3781 atatcccaac aaatatggat aatccaaatg taatttctga tgtagatata aaagttgttg
     3841 tagagtcaac ggaggatttg agtaagttaa ataaaaaaga tatttcatta tatattgatt
     3901 tatcacaagg ccctgaggaa gatagtaaat ataaaataaa atatgaaaca acatatcaat
     3961 ttaaaaaaat aaatatagaa cctaatattg ttgaagtgga atgaacaaat atttcgaata
     4021 tttagttgtt acaagatgcg acaaaaaaac ctataaaatt aagtttgtta attttatgtt
     4081 tttgttgcat cttgtttatt ttagttatat tataaaataa ggacaatgag atatttgtta
     4141 aatctaaaaa gcgctagata tatgaaaaaa gactgataaa tagatttaaa taaatatttt
     4201 gaattaaata tttcaattat ctctaggaaa acgttgctga acgagttata atttaattga
     4261 gatataaaaa gttctatttc aaaaaattca agatgaggtg gaaatgcatg agaagttttg
     4321 aagaagtaat taagtttgca aaagaaagag gacctaaaac tatatcagta gcatgttgcc
     4381 aagataaaga agttttaatg gcagttgaaa tggctagaaa agaaaaaata gcaaatgcca
     4441 ttttagtagg agatatagaa aagactaaag aaattgcaaa aagcatagac atggatatcg
     4501 aaaattatga actgatagat ataaaagatt tagcagaagc atctctaaaa tctgttgaat
     4561 tagtttcaca aggaaaagcc gacatggtaa tgaaaggctt agtagacaca tcaataatac
     4621 taaaagcagt tttaaataaa gaagtaggtc ttagaactgg aaatgtatta agtcacgtag
     4681 cagtatttga tgtagaggga tatgatagat tatttttcgt aactgacgca gctatgaact
     4741 tagctcctga tacaaatact aaaaagcaaa tcatagaaaa tgcttgcaca gtagcacatt
     4801 cattagatat aagtgaacca aaagttgctg caatatgcgc aaaagaaaaa gtaaatccaa
     4861 aaatgaaaga tacagttgaa gctaaagaac tagaagaaat gtatgaaaga ggagaaatca
     4921 aaggttgtat ggttggtgga ccttttgcaa ttgataatgc agtatcttta gaagcagcta
     4981 aacataaagg tataaatcat cctgtagcag gaagagctga tatattatta gccccagata
     5041 ttgaaggtgg taacatatta tataaagctt tggtattctt ctcaaaatca aaaaatgcag
     5101 gagttatagt tggggctaaa gcaccaataa tattaacttc tagagcagac agtgaagaaa
     5161 ctaaactaaa ctcaatagct ttaggtgttt taatggcagc aaaggcataa ttttaagggg
     5221 gaaataggca aatgagcaaa atatttaaaa tcttaacaat aaatcctggt tcgacatcaa
     5281 ctaaaatagc tgtatttgat aatgaggatt tagtatttga aaaaacttta agacattctt
     5341 cagaagaaat aggaaaatat gagaaggtgt ctgaccaatt tgaatttcgt aaacaagtaa
     5401 tagaagaagc tctaaaagaa ggtggagtaa aaacatctga attagatgct gtagtaggta
     5461 gaggaggact tcttaaacct ataaaaggtg gtacttattc agtaagtgct gctatgattg
     5521 aagatttaaa agtgggagtt ttaggagaac acgcttcaaa cctaggtgga ataatagcaa
     5581 aacaaatagg tgaagaagta aatgttcctt catacatagt agaccctgtt gttgtagatg
     5641 aattagaaga tgttgctaga atttctggta tgcctgaaat aagtagagca agtgtagtac
     5701 atgctttaaa tcaaaaggca atagcaagaa gatatgctag agaaataaac aagaaatatg
     5761 aagatataaa tcttatagtt gcacacatgg gtggaggagt ttctgttgga gctcataaaa
     5821 atggtaaaat agtagatgtt gcaaacgcat tagatggaga aggacctttc tctccagaaa
     5881 gaagtggtgg actaccagta ggtgcattag taaaaatgtg ctttagtgga aaatatactc
     5941 aagatgaaat taaaaagaaa ataaaaggta atggcggact agttgcatac ttaaacacta
     6001 atgatgctag agaagttgaa gaaagaattg aagctggtga tgaaaaagct aaattagtat
     6061 atgaagctat ggcatatcaa atctctaaag aaataggagc tagtgctgca gttcttaagg
     6121 gagatgtaaa agcaatatta ttaactggtg gaatcgcata ttcaaaaatg tttacagaaa
     6181 tgattgcaga tagagttaaa tttatagcag atgtaaaagt ttatccaggt gaagatgaaa
     6241 tgattgcatt agctcaaggt ggacttagag ttttaactgg tgaagaagag gctcaagttt
     6301 atgataacta atgataaaag aataagaatc ataactggtc actatggaag tggtaaaagt
     6361 gaatttgcga tgaactatgt tgttaagtta agagatatgg tttctggcaa agttgctata
     6421 gccgatttag atgtggtaaa tgtatacttt agaactagag aaaagaaaga gttaatgaaa
     6481 tccttaggta tacaaccaat agatagctca attaatgctc caacattaga tttaccagca
     6541 gtatcagcag aggtaatgtc tcctatggta gaccattcat acaattctgt tatagattta
     6601 ggtggagata atgtaggtgc tagggttata ggaagattta gtcatttgtt aaaagaaggc
     6661 gattatgata tgctttttgt aataaatgca aatagggaga aaactcaaac ttctgaagaa
     6721 gttatccaat atataaaaga aatagaaaaa tcttctaaat taaaggtaac aggtctaatt
     6781 aataatactc atcttattag gtttactact attgatgatg ttttaagagg tcagaaagtt
     6841 gcaaaagaag tttctgaaaa atgtaatata ccgataagat atgttgcatg tttagaaaat
     6901 cttgttgaac agttaccaaa agatttagaa ggagaaattt ttcctataaa attatatatg
     6961 agagaagact ggatgtagtt tgagctcaaa tatatagaaa gtttaaggag gcttaggtaa
     7021 atggctaaag gaaaagtatc ttttaatcaa gaacgttgta aaggttgtgg attgtgtgtt
     7081 gaagcatgtc cagtaaaaat catacaatta gactcaaatg taataaacaa aaaagggtat
     7141 aacccagcaa ctgtttttga aatggaaaaa tgcataggat gtgcaagttg tgcaactatg
     7201 tgtccagatg tagttataac agtagaaaga gattaattta attaatattt aagggggaat
     7261 tttaatggct aagatactta tgaaaggtaa cgaagcattt ggaaaagcag ctatagaagc
     7321 tggttgcaaa tatttcttcg gttacccaat aactccacaa agtgaattac cagaatattt
     7381 atcaagagag ttacctaaaa taggtggagc gttcgttcaa gctgaatcag aagtttctgc
     7441 gataaacatg gtttatggtg gagcaggtgc aggagcaaga gttatgactt cttcatcttc
     7501 accaggagtt gctttaaagc aagaaggtat aacatatgca gtaggagcag aagttccttg
     7561 tgttgtactt aatgtaatga gaggtggtcc aggacttgga agtatacaac cttcacaagc
     7621 tgactacttt atgtctacaa gaggtggagg aaatggagac tacagaactc cagtattcgc
     7681 accagctaca gttcaagaag ctgttgatat gataatggaa gcttttgacg ttgcagatta
     7741 ttacagatca ccagttatgg tagttgcaga tggtatgata ggtcaaatga tggagccagt
     7801 tgaatttaga gctcctgaga agaaaagaga attgcctcct aaagattggg caactgttgg
     7861 aactaaagga aaaagaaaac ctaatgtaat aaactctcta tacttagagc cagaagtact
     7921 agaagaccat tgttggcatt tacaagaaaa atttgatgca atggaaaaga atgaagtaca
     7981 atacgaaatg tacaaaacag aagatgctga atttgtattt gcagcttatg gaactacttc
     8041 aagagtagta aaaagcgcaa ttgatatatt aagagaagaa ggaataaaag caggtcttat
     8101 aagacctaaa gtattatggc cattcccatt cgaagcattc aaccaaattc ctaatgctag
     8161 aaacatattg actgttgaaa tgagtatggg acaaatggtg gaagatgtta aaatggcagt
     8221 agaaggtaaa ttaccagttt acttccatgg aagaccagga ggaatgactc caacaccagc
     8281 agaaatagtt gaaaaagcta aaaaaattat tgccggagaa ttggttgcag gaggtgctag
     8341 ataatggctg ttgtatttaa gaaaactgaa ggattacaag atactcaaac tcactactgt
     8401 ccaggatgta cacatggtat aatccataga ttagttggtg aagtattaga agaactagga
     8461 gtattaggtg atgctgtagg tgttgttcca gttggatgtt cagttttagg atataaatat
     8521 tttaactgtg acactcaaga agctgctcat ggtagagcgc cagctgctgc tacaggaata
     8581 aaaagagttc atcctgaaaa tacagtattc acttatcaag gtgatggaga cttagcttca
     8641 attggtactg ctgaaatagt tcatgcagct gctagaggag aaaaaataac tactatattt
     8701 gtaaacaata caacttatgg tatgactggt ggacaaatgg ctccaactac attagttgga
     8761 caaagagcta ctactgctca atcaggaaga aatgctgaaa ctcaaggtta tccaataaga
     8821 gtaagtgaaa tgttagctac attaacagga gctgtatttg tagaaagagt agcagttgat
     8881 actccagctc atgttagaca agctaaaaaa gctataaaga aagcattcca agttcaacaa
     8941 gcaggattag gatttggaat agtagaagta ttatctactt gtcctacaaa ctggggacta
     9001 gctccaaacg atgctttaca atggttaaga gacaacatga taccatacta tccattagga
     9061 aatttcaaaa atgttgaagt tgaggaggtg aagtaaatgt ctacagctag agtaatatgt
     9121 gcaggcttcg gaggccaagg tgttatgtct atgggacaat tacttactta tgcaggaatg
     9181 ctagaaggaa aagaagtttc ttggttacca tcttatggac cagaaatgcg tggaggtaca
     9241 gcaaactgtg cagtaactgt atctaatgag ccagttggtt ctccacttat aacagatgat
     9301 gcaactgcag ctatattatt aaatatacca gcatttgaaa aattcaaaga tgatgttatt
     9361 cctggtggaa aaataatagt aaatagttct ttaataaaag aaaaagttga tagaacagat
     9421 gtagatgttt attatatacc agcaaatgaa ttagcagctg aattaggaaa tgataaagtt
     9481 gctaacatga taatgttagg cgcttactta aaagtatctg atactgtaga tatagaatct
     9541 gtactagaag cattcaaaaa agttttcgga cctagaaaag aaaaattcgt tccattaaac
     9601 agagaagctt tacaaaaagg tatggatgct acatgttcta aagcaactaa ctaataaaga
     9661 aaaagatgtc aaaaaaaaga caatagaatt atttctattg tcttttttgc tgataagtat
     9721 taaaatattt ttatgactga aattaagcct ataatgatat tttatgaact ataaaaatat
     9781 aatacaaatt acaagctttt caattatgaa aggagaatct ttagtgagaa agtattttgg
     9841 aacagatgga gttagaggag tagcaaatac agaactaaca tgtgatttag catataaact
     9901 tggtagagct ggtggatttg tcttagctca aggagaccac agagtaaaag tagtagtagg
     9961 taaggatact agaatatcag gtgatatgtt ggaagcatct ttaatagcag gacttatgtc
    10021 tgttggatgt gatgttatta ctgttggaat aataccaaca cctgcagtgg catatttaac
    10081 tagaaaatat ggtgctgatt gtggtgtggt tatatctgca tctcacaatc ctgttgaata
    10141 taatggaatc aaattcttta ataagaatgg atataaatta gatgatgaaa ttgaattgaa
    10201 aatagaagaa tatatagacg atatagacaa aattgattgt cttccaattg gtgaaaatgt
    10261 aggaagaaaa ttacatgaac attgtgcaca aagagactat gtagactacc taaaatcaat
    10321 aataagcact gactttaagg gattaaaagt agttttggac tgtgctaatg gtgcgtcata
    10381 taaagtggca ccaatagtgt ttgatgaact aggagcaagt gtaatttcaa taaatagttt
    10441 accagatgga aataatataa actataaatg tggctctaca catccagaac aacttcaaag
    10501 ggctgttttg gaacataatg ctgacttagg tcttgcatat gatggagatg ctgatagatt
    10561 aattgcagta aatgaaaagg gtcaaattgt tgatggagac catataatga tattgagtgc
    10621 cttaaattta aagaaaaata ataaattggc acaagatact ttggtagtta cagttatgag
    10681 taatataggg ttgacaatag ctgctaagga aaatggaata aatttatcta ctacagcagt
    10741 tggagacaga tatgttttag aagatatggt taaaaatgga tataatcttg gtggagagca
    10801 atctggacat atgatatttt tagattacaa cactactgga gatggagtgc ttagttctct
    10861 aatacttgca aatataattt tgcaagaaaa gaaaccttta tcagaaattg catcaataat
    10921 gagccaatac ccacaagtat tagtaaatgc aacaataaaa aatgaaaata agaacaaata
    10981 tatggaatat ccagaaataa aaactgagat agaaagaata gaatctattt tagacggaaa
    11041 tggtagagtt ttgataagac catcaggaac tgagccatta gttagagtta tgttagaggg
    11101 taaagaggaa ggtcaaataa aagaattagc aacagactta gctaatctta tacaagaaaa
    11161 attatcgtaa tttaaagggt attctcagat acaaatactg aggacacctt tctaagcgcc
    11221 agaacttagt tatagacata attattgagt aatataggaa ctaagttgac gaggtcagag
    11281 ttaatcgaat tgtcggcgga tgctctgcgg tgtgtttacc atcgaaagac ttctttaaat
    11341 aataggagca atcctatgga caaagggaag ccaaagtaaa cttatatgac ctaactctta
    11401 aaatatataa ataatatttt taggaggaca caaataatgt gtggaatagt tggatattta
    11461 ggaagtagaa aggcagcaga agttatagta gaagggcttt ctaagctaga gtacagaggt
    11521 tatgactcag caggagtagc agttaatagt tctaatgaaa aagaacttaa tattagaaag
    11581 tttaaaggta gactttctgt acttgcagaa gacttagaaa agaatcctat tgatggaaat
    11641 ttaggtatag ggcatacgag atgggcaact catggagaac catctgatgt aaactcacat
    11701 cctcatttta atcaagccaa aacaatagca gttgttcata atgggataat agaaaactat
    11761 atggaaataa aagaagaatt aatttctgaa ggtgttaagt ttgaatctca aacagataca
    11821 gaggtaatag cacatttagt agataaatac tatgagggta atttattaga tgctgtatac
    11881 aaaactatat caaaattaag aggagcttat gcattaggtg ttatatgtaa agaacatgga
    11941 aatgaattag ttgcagtaag aaaagatagt cctcttgtag ttggtgttgg tgaaggtgaa
    12001 aactttatag catcagatat acctgcatta ttaaaatata ctagagatgt ttatttctta
    12061 gaaaatggag aagtagttca tctaaaagac gaaaatgtaa ccgtttatga ctctaataga
    12121 aatcttgttg aaaaagaagt atttcatgta acttgggatg tagaagctgc gtctaaaggt
    12181 ggatatgact actttatgtc aaaagaaatt catgagcaac caacaggagt tagagaaact
    12241 ttagaaagaa gacttgatga taatggaaat ataattctag acagtataaa tatttctaaa
    12301 gaagacttag aaaaaataaa taaagtttat atagtggctt gtggaacagc atataatgct
    12361 ggattacttg gtaagtatgc catagaaaaa tttgtaaata taccagtaat aactgatata
    12421 gcatcagaat ttagatatag cgacccattt gtagatgaaa attcattagt aatattagtt
    12481 agtcaatctg gtgagacagc agatactcta gcagtattaa gagattcaaa ggctaaagga
    12541 gcaagaatac tttcaataac taacgttgta ggttcatcta tagcaagaga atcagatgat
    12601 gtattctata cttgggcagg acctgaagta gcagttgcat caactaaggc atatactact
    12661 caaataactt ctttatacat gatagcttta gactttgcta tcaaaaaagg aacaataact
    12721 agagaatttt atgatagtat gatttctaag atgaaagaaa taccatcaaa aatacaagaa
    12781 atactagaca atgaagaata tataaaagaa gttgctaaaa cagttgttag ttctgaacat
    12841 gcattctatt taggaagagg tatagactat agcttagcaa tggaaggttc attgaaatta
    12901 aaagagattt catatataca tgcagaagct tttgcagcag gagagctaaa gcatggaaca
    12961 atagctttaa tagaaaaagg aactcctgta attgccatag ctactcaaga aaagttattt
    13021 gaaaaaatgg tttctaatat ggaagaagta agagcaagag gagcttatgt agtagcaata
    13081 gctcaaagcc ataataagga tgttgagaaa gctgctgata aaataatcta tataccaaat
    13141 tcagatgata ttttatcacc aatattagca gttgtaccaa tgcaattact tgcttatcat
    13201 gtctctgtac taagaggatg cgatgtagat aagccaagaa atctagctaa atcagtaaca
    13261 gttgaataaa aacagattaa aaattaaata gataatattt attgaaaaga actcatcctg
    13321 ttatttgtat tttataggat gagtttttta gataaatatt aaatttgttt tgtttttgct
    13381 ataataaatt ttataaaaag attgatatta aatcatctgt aaaatgataa atatatagac
    13441 cataccaggt atagggaacg ataaatatag ttcatataaa atatagtgag tacttaataa
    13501 acatatagtc aatatcagat aaaaaaaata atagcttatg agaaaaatta aaatacaagg
    13561 tattagtaac cttgtgataa gataaagtga gtagtttaaa ataagaatat aaggaactat
    13621 ttataaaaga atataatgaa gctaatagag tataagaaaa ttactttatt ttagtaaaat
    13681 aatttcaaat aaaaattttt ttaaattggg agtataaatt atgacaaatg attttaaaaa
    13741 agaaaagaaa gaagattatt ttgttaatct taaggcaata tctactgaag ttttacaaga
    13801 aaaagtgcaa gataacgctt gggttatagt tgatactcgc ttaaatgatg cttataatgg
    13861 ctggaagtta gatggagtaa gaagaggtgg acatataaaa ggagcagtag atttttctgc
    13921 taattggtta agtgtatact tagatagaaa agatgaagtg ttagaacaag cattgaaaac
    13981 taaaagaatt gatttagata aaaatatagt attgtatgat gcaaatggaa aagatgcttt
    14041 ggttgtagca gattatttat ctaaaaaagg atataaatat ctatataaat atgatattaa
    14101 acaatgggca gacgacgaaa atcttccaat ggaaagatat aaaaattatc aaatgatagt
    14161 tcctgctttt ataataaaag atatattaga tggaaaaatt cctgagacat ttgaagattc
    14221 aaagaatata aaaatgattg aagcgagttg gggagaagaa tcgtatacaa aagggcatat
    14281 acctacaagt gttcatgtaa atactgatat tatagaacct cctccaacat ggatgcttga
    14341 taatgatgat aacttaacta agtttgcttt agactatgga ttaacaaagg atgatactgt
    14401 aatagtttca agtagtacac ctatggcatc ttatagacta gctgttatat tgagatatat
    14461 aggagtaaaa gatgtaagag ttttgaatgg tggaacaaac tcatggttat cagcaggata
    14521 tgaattagaa tttattagta atccaaaaca ttcatgtact aattttggag cagatatacc
    14581 agttaactca caattagttg ttactacatc tgaattaaga caaaaactaa aagaaaaaaa
    14641 taagtttata ctagtagata accgtacttg ggatgaacat ataggaaaag tatctggata
    14701 tacatattat gataaaaaag ggcgtatacc aggagcttta tatggacatt caggtagtga
    14761 tagtgtttca ctagaagaat atcgtaatat tgataataca atgagaaata aatatgagat
    14821 tttagaaatg tgggacaagg aaaatataga tgtgaataaa cagcttatat ttatgtgtgg
    14881 aagtggatgg cgtgctgctg aagtattaac atatgcaaat gtcataggag tggaaaatac
    14941 ctccctttat agtgatggat ggatgggctg gagtcttgat aattcaaatt taattgaagt
    15001 gggagaacac aaataaagtt tttgaaagta caatagagtt gtttaaaaca aaggtgtttt
    15061 tgatttatta ggaagagtgt gtaaattgtg tattgggaag tgtacttagg tatgttcatt
    15121 tggagctatg gaaattgata aaaaagtatt atctgtttat tttgttggta aaatatgaat
    15181 agattataga ttatagatta tagattatag attatagatt taagtataga ctaagtaatt
    15241 tatattacag aagagaatat ataagaatta atgcaagatg tatttgatac atataagaaa
    15301 ttggcaagtc caggagaagg atagagaaaa ttgagattga agagtttgaa agagattatt
    15361 agatgaaaaa tacaaaaatg tataaaaata catattgaga aagtatatct ctcttaaatt
    15421 tgttaataat tctattatac agaaaaacat atttaagggg gattttttac tatgaaaaaa
    15481 gtaaaattag ctataagctt ttttatatta atgattatag gaatgacaat atcttatgca
    15541 gatacaagta taaatactag atacaataga ttaagtaaag ctcttgaaat atcagaagca
    15601 aattttaaat tttataatgt taaaattaat tctgttataa atcataattt atcaaataaa
    15661 gaatttcata atatttttat agaaataatt aacaatctaa agttggataa aaaaggaata
    15721 aattggaatc aaaaagggtc tggcgataaa atacaattat gtgctactat aaaaggtagc
    15781 gataaagaaa tttctataaa aggaataaat aaaaataaca aagaaagtta catagtagtt
    15841 gacatattgg ataacaaagt atataaacat aaagaggtta tttatagtaa cgtagaaaat
    15901 gtgttaaata aatattcatc tcaagtagat gtgtctgctt gtattgttgg agaatacaca
    15961 aaaaggttac aaaacaacaa atatgatgat attttacaaa aaatattata taatatgagt
    16021 gctaagcaaa tagatgaagt taaagacgta aactttttat ctataacagc atatagtaaa
    16081 gtattaaatg attatgaatt agaatattta ggggaaaaaa ttaatttaaa tataggaatt
    16141 aaatataatg aggatgacgg aaaaacactc atatatattg ccacaccaat tataaaatta
    16201 gattattaat tatgaggaga tcaaaaaata tggctaagat aatagtaaaa aagagtaatc
    16261 cacttaaagg tagtgttaaa atagatggtg caaaaaatgc agttttacca ataatagcag
    16321 cgactttatt agcaaacgga aaatcaacat taaatggagt accaaattta agagatgttc
    16381 atgttatatc agatttatta agacatgtag gagcagaagt tgaatataaa gaaaatacac
    16441 ttactgttga tgcaagcaat ataaaaactt gtgaagctcc atatgagctt gtaagaaaga
    16501 tgagagcttc ttttttggtt atgggaccat tgcttgctag atttaatagt acgaaaattt
    16561 ctatgccagg aggatgtgct ataggaacaa gacctataga tttacattta aaaggattta
    16621 aagccttagg tgcaaagata gaaatggacc atggttttgt tgaagcagca acagaaaagt
    16681 tagtaggaaa taaattatat ttagatttcc catctgttgg agcaactgaa aacataatga
    16741 tggcagcttc tttagcagaa gggactacaa taatagagaa tgcagcagaa gagccagaaa
    16801 tagtagattt agcaaacttc ttgaacgaaa tgggagcaga tgttaaaggt gcaggtacta
    16861 atactataaa aataaaaggt gttaaagagt taaaaggagc agaacataat gtaattccag
    16921 acagaataga agcagcaact tatatggttg cagcagctat gacaaaaggt gatataactg
    16981 ttgaaaatgt tttaatggag catttaaaac cagttgtagc taaattaaga gaagctggtt
    17041 gtgaaataac agaaatggac aattctgtaa gagttgtagg tccaaaagta ttaaaaccaa
    17101 tagatataaa aactttacca catccaggat ttcctacaga tgttcaagca caatttatgg
    17161 ctatgcttac tgtagccaat ggaactggtg tagttataga aactgttttt gaaaatagat
    17221 ttatgcatgt tgctgaattt aatagaatgg gtgctaatat aaaaatagat ggaagaagtg
    17281 cggttgttaa tggtgtagat gagctacacg gtgcagcagt aaacgctact gatttaagag
    17341 caggggcagc ccttatttta tgcggtctta tagcagaagg agaaactcaa ataggagaaa
    17401 tataccatat ccaaagaggt tatgttgata tagataagaa gataacagca ctaggtggtc
    17461 aaatcgaaat agtagaagat taataattgg tttatagaag aaagttataa attaataggg
    17521 ttgttcatat acttaaatac aaggttttaa aagctaagga ggagagtata tgaagaaccc
    17581 attggttgtt ttgttaggat ttgtgacttg ctctgtgtta gttccatcac ttataacttt
    17641 agtttcttat aaaaatgtag aattaactga aaaaccagag agcccagtat ctataaacaa
    17701 aactattaaa aaaagtgata ttgaagacaa aggtaataaa gaagaaaaaa atacagtgaa
    17761 ctatgaaact gtaaataaaa aagcaccaat tatcaatgta tataatcata taacagggaa
    17821 aaccgaaaag atggacatgg agaactattt atgtggagta ctagctggtg agatgtcttc
    17881 agagtttgat atagaagctt taaaagctca atctgtagca gctagaacat atgtagtata
    17941 taaacaggaa catggtaaat ctagtaaaca taaaaatgca gtagtatgta ctgattataa
    18001 acattgtcaa gaatataaga gttatgatac tcttaaaaaa ttaaatggtg aagaatggat
    18061 taagaataaa tattccaaaa tccaagaagc agttagagga actaaaggtc aaataataac
    18121 ttataatgat aaggcgatac ttcctcttta tttttctaca tcttcaggaa aaacagaaaa
    18181 tagtgaagaa gtattttctg caaaatatcc ttatttaaag tctgtggaaa gtccttatga
    18241 taagtattcc cctaaatttg cgtcaacact taaaataagt aacacagatt ttgttaaaag
    18301 tcttagaagg gcatatagca ctattgtgat agatgtaaat aatttaagta aacaagtttc
    18361 aataactaaa agaagtgatg ccggaactgt agaaaaaata aaattaggaa ataaagaatt
    18421 gactggaaaa gacattagaa ctgtttttaa attgaactct gcaaactttg acataaaatt
    18481 tggagaagga tacatagatt tcgtggtaaa aggatatggt catggtgttg gtatgagcca
    18541 gtggggagca gaaggaatgg cagaagaggg ctataaatat tatgatatat tatcacatta
    18601 ctacacagat acaaaaataa aagatatata ctaataattg tataaaatgc caaaaacacg
    18661 tcaataatcc aaatgtagaa atatatttgg aggtgcaaaa tgaagaaaaa gctgttagaa
    18721 aaagatggtt tttatttatc tttgtttgta tgtgtttgtt tattagcagt aggtggagtt
    18781 tggtttacaa ataataatgt agataaattg gcttctaata aaggaattat ggaaaatgct
    18841 aataaagata gtgaagagga aatacattta attgaaaaag acaagaaaga tgctatccct
    18901 actgcaacgg attcaaaaca aaacttagaa aaagctaagt caaaagagga aaataaatcg
    18961 agtacaacaa aattaaatta cattggagat aaagtaataa gaggatattc agaaaaagag
    19021 cctagttatt ctaaaacact tgatgtttgg gaaactcata aaggtgtaga tattagttgt
    19081 actaaaggca aagaagtaaa atctctatta aacggaatag tagtagatgt atttgatgat
    19141 gaagaatatg gacaatcagt caaaataaaa agtgacaata acattgtagt agtatattca
    19201 aatctagata aaaatgttag tgttaagaaa gaacagaagg taacagaagg acaatctcta
    19261 ggtactgtag gtagtacttc tcaaatagaa agtgaagaag gtatacatgt gcatttagag
    19321 gcgtatagtg gagaaaaatc tatagaccca atgagtctaa ttaagtaata gtataacaag
    19381 tttaatcata tttggttaag cttgttatat tttttggtgt ttttacatat atatcattaa
    19441 agctattatg tgaggggggt aatctttgag atctcatata gaggaaaggg ccatagtggt
    19501 agcaaaatat atacttgaaa aaaacactac agtaagacaa accgccaaga catttggagt
    19561 aagtaaaagt acaattcata aggatgttac agaaagattg aaggaaataa atccatccct
    19621 tgccaaagaa gttaaaaatg tgctggacaa aaataaatca gagagacata ttagaggggg
    19681 attagcaacg aaattaaaat atgagaagga aaataaaaaa atgtaggtta aaaacctact
    19741 ttttttattg aattaaatta taaaaagaaa tatattaagt aattataaat agcaaaataa
    19801 taatactctc aaaagattta ttttctcttg attaaagcga gatttttgta tatgctttat
    19861 tataggagta ttaaaatagg cttataatat aatctaatat tataaataaa ggagttgaaa
    19921 aaatggctgg agctgatata ggcatagatt taggtactgc aaatgtgctt gtttatgtta
    19981 gtggtaaggg aatcgtttta gaagaaccat ctgttgtggc gatagacaaa agaacagatt
    20041 ctgttttagc tgtgggagaa gaagctaaaa agatgatagg gagaacacca ggtaatatag
    20101 tcgctataag acctttaaga gatggtgtaa tttcagatta tgatgtaact gagaagatgt
    20161 taaaatcttt tatagataag atagttgata aaaagggatt tggaagattt tttatgccaa
    20221 gaattatggt atgtgtacca acaggtgtca cagaagtgga aaaaagagca gtagaagatg
    20281 ctacgagaca agctggagct agagaagtat atatcataga agaaccaata gcagctgcca
    20341 taggagctgg tgtagacata tcacagccta atggtaatat gattatagat attggtggag
    20401 gtactgtaga tatagctgta atatctctag gaggagctgt tgtaagtgaa tctataaagg
    20461 ttggtggaga tagatttgat gatgctatag ttaaatatat gaaaaaacaa cacaatcttc
    20521 ttataggaga aagaacagca gagaaaatca agtttgaaat aggttctgca tttaagagag
    20581 aagaagaaaa atatatgaag ataacaggaa gaaatttaat tacaggactt cctagttcta
    20641 taactataaa ttcaacagaa atgttggaag cacttagaga atgtgttgag caaatagtag
    20701 ttgctactca tgctgttttg gaaaaaacgc ctcctgagtt agcagcagat ataggagatg
    20761 tcggtataat aatgactgga ggaggttcat tattatatgg attagataaa ataatagaac
    20821 agaggacagg aataactgtt acaatagcag atgaaccatt atcttgtgtt gcaaaaggta
    20881 caggaatagc tttaggttca atagacttac ttgaaactgg tgggtctttc aaaagaaaat
    20941 aattaggaga gtttagaatg ttaaatatag atgaaataaa aaaattaata ccacatagat
    21001 atccattttt attagtagac aagattacag agctagaagt tgggaaaaga gctgttggta
    21061 taaagaatgt aacagttaat gagccatttt tccaaggtca ctttccagaa tatcctttga
    21121 tgccaggagt actaatagta gaagctctag cacaagtatg tggagtggca atgatgtctg
    21181 tagaggaaaa caaaggaaaa ctaggtgtat ttgcaggtat agataaagtt agaatcaaga
    21241 gagaagtaag accaggagat acattaacta tggaagttga aatgacaact cttagaaaaa
    21301 atatagctaa ggcagatgct aaggcatatg taggtgaaga acttgtctgt aaaggtgaat
    21361 taatgtttgc tttagtagaa aaataaaaac ttgaaatata aaagtaaaaa ggtagataat
    21421 ttggagtatt taatcagata ctctaaaata tctacctttt tatataaaga attttatcat
    21481 ttagtttata tttagattag gtggatacta ataaagccaa ggctattgtt tcagctaaat
    21541 ctaatataaa gctaaggcgt atattattaa atgaaagttt attattttca tcaattttat
    21601 ctacaattcc tactatagaa taatttccta tttgaggtag ttttttacca acaccttttc
    21661 cgggcaggat aggtccttct ctaatctgta tattacctat gttactctgc gaccccaagc
    21721 aagcatctat agccaagaag ttaccttgta tatgtgtatt ttttatagta tctaaagact
    21781 catatatatt taaggcgtga atagggttat ctagtgtacc gtaaacgggg tatttaaagt
    21841 tactgttctt aagtattgtt ccaactaaag ggcctaaagt atcacctata gccctgtctg
    21901 ttcctatgca aacaacaatc gtattttcat taattatagt ttttagaact gaacttagca
    21961 tctgtataac atcatcatcg ctgtagttta ctttagctaa atacaatagg aacaccccct
    22021 tcaaaaaaat atatgaataa aaagcattaa aaagacctaa ctttaaattt cagatagata
    22081 gaaaatttta tttgtcttac aaaaaaagga atagaaaaaa tatgactaaa aatattgaca
    22141 aaattttaaa aaaatagtaa gctagtaaag taaaacaaaa cttaaatctt gattacctta
    22201 tcaagagagg ctgagggata ggccctgtga agcccagcaa ccacccaaat gggaaaaggt
    22261 gctaattcct acaagacaaa agtcttgaaa gatgaggtca gttaattagt atgtaattac
    22321 gattattaac ctcttcttat aaaggagagg tttttttatt ttgagtaaat ttctaaaatt
    22381 aaaaataagg taaaacctat aataaaaatt aagaataaat aagggggaat aaaaatggca
    22441 agacatttat ttacgtcaga atcagttaca gaaggacatc ctgataagat atgtgaccaa
    22501 atatcagatt caatattaga tgcattatta gaaaaagacc cacaatctag agtagcttgt
    22561 gaaactacag ttactactgg tttagtttta gtagcaggag aaataagtac atctgcttat
    22621 gtagacattc ctaaattagt aagagaaaca gtaagagaaa ttggatatac aagagcaaaa
    22681 tatggatttg attgtgatac ttgcgcagtt ataacttcta ttgatgaaca atctggagat
    22741 atagctatgg gagttgatga aggtctagaa agtaagactg gagaagagat agaagaagaa
    22801 atagaaaaag ttggagctgg agaccaaggt ataatgtttg gatttgcatg taatgagact
    22861 ccagaattaa tgccacttcc gatatctttg gcacataaat tatcaagaag acttacagaa
    22921 gttagaaaaa caggtttagt tgattattta agaccagatg gaaaaactca agttactgtt
    22981 gaatatgaag gaagtaaagc tgtaagagta catacagttc ttatatcagc tcaacattgt
    23041 gaaacagtat caaatgataa aataagagaa gatttaatta atcatgttat taaggaagtt
    23101 ataccagcag aattacttga tgaagagact aaaatttata taaatccaac aggaagattc
    23161 gttataggag gaccacaagg agatacaggt cttacaggaa gaaaaataat aatagatact
    23221 tatggtggat attctagaca tggtggagga gctttctcag gaaaagaccc tacaaaagtt
    23281 gatagatctg ctgcttatgc agctagatat gttgcaaaaa atattgttgc ggctggtctt
    23341 gcagacaaat gtgagataga acttgcatat gctataggta tagctagacc attatctata
    23401 tttatagata cttttgggac aggcaaggtt tcagaagaaa aacttgttga attagtaaat
    23461 aaacactttg atttaagacc gggagcgata ataagagact tagatttaag aaagcctctt
    23521 tataaaaagg ttgctgctta tggtcacttt ggtagaactg atatagattt accttgggaa
    23581 agaactgaca aggttgagca gttaagaaaa gatgctttag gtgaataatt tttaactaaa
    23641 aagctgttag aatgtaattt tatgattact ttctaacagc ttttttatta tgagttaaat
    23701 atttttaata aatgttaatt tatcatcata taatgaataa acagtattta atctggtcaa
    23761 tacattttat ataatactct actaaattta tacatattat gggatatatg ttaaaggatg
    23821 gttaagaatg tgttaactaa tgttaaataa ttaaaaatat attgaaatat cacaataatg
    23881 agtctaatat aatcttaata aacttacact ttaaaaataa ttttaattgg gggggagtag
    23941 tgtatgtcag gcaaagtaaa tattaaaacg gttataagtt ttgctggagc ttatgttgct
    24001 actgtaatag gttctggatt tgcaactggt caagaaatac tgcaattttt tacgttttat
    24061 ggatatgcgg gtattatagg tggaataatc tctatggtac tattttcatg gtttggagca
    24121 gaagttatag ataagggaag agaattgaaa cttaaggagc caataaaaat ttatcaagta
    24181 tattgtggaa aatatcttgg aacatttttt gaatggtttg gtccattgtt tttatttgga
    24241 acattagtaa taatgattgc aggagcagga gctacattat cagagtacta tggattaaat
    24301 ccatatgtag gtaggattgg tatggcaatc gtttccctaa taactgtttc attaggtttg
    24361 acaaggcttt ctaaaatact aggtaatata ggacctataa taataatatt tacactttta
    24421 gtaggagcaa ttagtttatt tagcaatatt gatgctttat caaatgctgg aaatatggtt
    24481 gatagtttaa atattaaaac tgctacatca aatggatatt tctcgggagt actttatact
    24541 tgttacaatg taataatagt aataacattc ttgacaggaa tgggagcttc tgcagtaaat
    24601 aagaaagatg ctgtatgggg tggtatagta ggtggagtag cacttatggc agcagctata
    24661 atgatgaatt tggcattact ttcagacata ggaaatatat acacaaaaga gataccagca
    24721 ctttacttag ctgataagat atctccaata ataggtattt tattctcagt agtactttta
    24781 ctaggaatat acactacagc tgtaccattg ttatggtcag taacaaaccg atttgtagaa
    24841 gatgaccacc ctaagtttaa aattataaca atagtggtaa gtatattagc ttgtatagga
    24901 ggtttactgc catttgataa gttagtaggt acactatatc catatacagg ttacatggga
    24961 attttaattt tactatgtat gctttataga agaattacta aaactgaggg gtacaaggag
    25021 aataaatctg aaataagtta gtactgtaat atacttatga aatttaattt atactctaaa
    25081 tatagagtaa ttaaaataaa atagctattt aaatatggta cctgtatttc atggtaccat
    25141 attttatgga tgcagatttt ttttgtttct gataaaatgt ataatagttg gaaattacgt
    25201 taagtataaa aatataaaac ttttctacga ttatcatagt tttaatatat aaaattatac
    25261 taacatttaa atggagcgat tattagtatt tttaggagat aaaaatggaa aaattagaag
    25321 gaatgataag tgaaatagtc tttaaaaacg aagataatgg ttatacaata gctcacctgg
    25381 taaatgaaaa tgatgaaatt gtggttgtag gatgtatgcc tactttggca attggagaaa
    25441 gcatagaagt tgaaggaaag tgggtaaatc ataagattta tgggactcaa tttgaagtga
    25501 atagttttat gccagtaaca ccatcatcct tagaaggaat ttatgtttac ttatcttctg
    25561 gaatgataca tggtattggt gaaaaaatgg caaaaagaat aattgataag tttggtgtag
    25621 atacgttaga ggtgatacaa aattcaccag aaaaactaca agaagtagaa ggtataggga
    25681 gtaaaaaagt aaaacaaata gtaaaaagtt atgaagaaga cagagaattg agaaatataa
    25741 taatacagct atctcctttt ggaataacac caaattactg cctaaaaata tataaaaaat
    25801 ataaaagcag tgcaatagaa gttataaata aaaacccata ccaacttgct gaagatataa
    25861 gaggaattgg atttaaagta gctgatagta tagcaagtaa aattggtata gataaaaact
    25921 ctaaagatag aatttgtcag ggaatcttat acactttaaa taaatcttta agtaatgggc
    25981 atacatattt accagagcat gttctaatac aagattcgga aaaattgttg gagctaaatg
    26041 gagaaatact aaaagagtgt gtaatgatgc tagtttataa tcaaaaaata catatagaga
    26101 aagtaaacaa tgaaaatctt atatacctta tgccatacta tttagctgaa aatggagtat
    26161 gtagtcaaat agtaaaatta tcacagtatg aatttgaaga cttaaaaata gatatagata
    26221 acgagattaa tgttttagaa gaagataaga aaattaagtt agcagaaaaa caaattttag
    26281 cagtcaaaga atctgttaat agtggagtat taattataac tggtggacca ggaactggta
    26341 aaacaactac cattaatact atcatagata tatttgaaaa taatggtaaa agtgtgactt
    26401 tggcagctcc aacgggaaga gcagcaaaga gaatgagtga aacttctaat aaagaagcaa
    26461 aaactataca taggttgcta gaaatgggat tttcaacaga tgatgattta acttttttta
    26521 aggatgaaga agacccaata aattcagatg taataattgt tgatgaggta tcaatggtag
    26581 acattatctt aatgtacaat ttacttagag ctataaagtt aggaacaaga gtaattttag
    26641 ttggagatag tgaccaatta ccttctgtag gtgctggaaa tgtacttaag gatatgatta
    26701 attctaatat tataaatgta gttaaattaa atgaaatatt tagacaagcg caagaaagta
    26761 tgataattgt aaatgcccat aagataaaca atggagaacc actttatcta aatacaaaag
    26821 gtaaagattt cttttttata agaaaatcaa ctaatgaaga aattttaaat gaaataatag
    26881 gattggttaa tgaaagatta cctaaatttt ataaagttga gaaattaaaa gatatacaag
    26941 ttttatcttc aatgagaaaa ggtgaattgg gagttactaa tctcaatata gagttacaga
    27001 agtatcttaa caaaaaagaa aaatttaaag ttgaagaaag tttttctaaa agattattta
    27061 gagttggcga taaggtaatg caagtaaaaa ataattatac aaaaaaatgg gaaacagaag
    27121 accaaaaaga aagtggagaa ggtatctata atggagatat aggatatgtt tatcacatag
    27181 ataaagataa gaaaactatt tatatattat ttgaccagac taaaattgta tcatacctct
    27241 atgatgaatt agatgaaatt gaccatagtt tttgtacaac aatacataaa agccagggaa
    27301 gtgaatttcc agttgtagta ttgccaatag cgtgggctcc tcctatgtta cttagccgta
    27361 atttattata tacagcagta acaagagcaa aaaaattagt tgtattagtt ggagatgtga
    27421 aatatcttga atatatgata aaaaataata gagttaatca aaggtattca aatttaggat
    27481 ataagctaaa taaatttaaa caagaagggt tgttgataga ataaatatga aaatatgcaa
    27541 tgataatttt attaagaatt ttaaaaatat gattaataaa tgtttggatt ttatatatcc
    27601 tgagaatata agttgcataa tttgtgataa atccataaag aaaacaaata cttattcaat
    27661 atgtaaaagt tgttttaaag aaatgaattt tatacaagat ggttgcatga agtgtggaaa
    27721 accaataata cgtcattcaa ttgaaaaaga atttattgaa gagtgtagtt attgttttaa
    27781 taaagacttt tattttgata aatcaatatc ttgtattgaa tacaatgatg tgacaaaaaa
    27841 gatggtgtta gggctgaagt ataaccaaaa aacctttatg gctaagtata tagctcaaat
    27901 aatgaaagaa aaattatatt tagaaaacat aaaatttgat tatatattat ttgttccact
    27961 tcataaaaaa aggctaaata aaagaggttt taatcaggca caaaaaatag catttaattt
    28021 aagtaaaata gtaaatattc cattattaga ttgcataagt agaaaaaaat atacaagaat
    28081 gctatataaa ttgaataaga aagaacgaaa agaagaatta aaaaatgtat ttgttgttaa
    28141 agaaaatgtt aaattaataa ataataaaaa tatactttta atagatgata tatttactac
    28201 tggtctaaca acaaacgaaa tttctaaact tttaaagtta tcaggtgcta ataaggtttt
    28261 tgtattgact ctacttacaa aagcaaatga taattatatt atggaataat cttatatatt
    28321 gacaatttta gtttaaaata ttaaaatgaa taagtgatat ttaggtctgt ggttgaaagt
    28381 ccaagccagt cgcaggcaaa gggatccacg taagttaaca ctaaaaattg ggttaatgat
    28441 catggtgcgg cttagaagta agacctaccg ataataaaat cgagagagag tagtagtgcg
    28501 gcgaacccaa gcgaaccctc cagtaggcga gtgtggggtc aaaaaccagg tcagctaaat
    28561 atcatattaa aagagagcta gcataccttt atgtatagct cttttttata tataataaat
    28621 ttaggttgag tcataaagag gtgatatgtt tgaataaaag acaagaaaat ttattaacaa
    28681 cattgctatt aaaagaagag tttataaatg ttaataagat tgccaatgat tttgagtgtt
    28741 cagaaaggac aataagaaat gattgcaaat atattgatga atggttatct actttttgta
    28801 atgcttatat caaaagaaaa cctaatattg ggattaaatt tagtggagat ggaacagaca
    28861 aaatactggt aaatcagaag ttgcgaggtg aagttaaaag agtaaatagt gagcttcata
    28921 agcaagttga gatattaaat ttactacttt gtaataataa aaaagttact ttaaatgatt
    28981 tatctaataa gttgtacata aataaaaata ttgtcagaga agaaataagt aaattatcag
    29041 tagcattaaa taattataat ttaaatatct cgactaaaaa aggcagtggt atatatattg
    29101 aaggggatga gaaagacata agaataatgt tagtatcatt cttatttaaa tatatagata
    29161 aatacagatt aaacatagat gaaatagagt acttccatat agtagatata ataattgtaa
    29221 aaaacataat gcttactata gaggaggata tggatataag acttacagat atatcattca
    29281 agcaattgat gctatttttg cttataaata taatacgaat aaggctggga aattctttaa
    29341 aagtatcaaa tactaagaaa gagcttaagc taaaaatgct tatattggat atagaagatg
    29401 aattgaaatc taatttatcc atagtattaa attatgaaga aaaattgttt atagaatctt
    29461 taatattggg tatgaacaaa caaataaata gtaataatat taagaaatct ttatatctaa
    29521 atgaagaatt agcaagttat acaaaaaaaa ttataaattt agtatcagaa gaatcaggaa
    29581 taaattttaa taatgatata ttactttatg aacaattaat atgtcatttg aatgttacaa
    29641 tgcatcaaat gaaaagcaat gtttacttag aaaatccact tttagataac ataaaaacta
    29701 aattttgttt tttatttaca gtaatatcaa gttcaataga aattgaatta aaaggaaaac
    29761 aaataacgga agatgaaatt ggttatctta cacttcactt tcaaacctct ctagaaagaa
    29821 aatacagcaa aagggaatca aataaaaagg caagtattgt atgtccattt agttttggag
    29881 taagtatgct attaaaagta aagatagaaa agagatttga taacataaaa attattgaaa
    29941 cattaagaga ggaagattta aaaagagata actttaataa gactatagat tttataattg
    30001 catttcaaga gtatgaaaat atccaaaaac caatttttat aacaacacca ttatttactg
    30061 atgaggatga gaataaactt aaagagttca caaacaaaat aaaagaaaaa gacacttcat
    30121 ataaacttat gaacagatta atgatgtctg attatttaat aagagagtta gaacatgatg
    30181 atatttatga agctataaca tatttaacta atttattaat tgaaaaacat tatgcagaaa
    30241 aagagtattt acaatctatt ataacaagag aaaagtcata tccaaccaat gttggaaaag
    30301 gaatattatt ccctcatgga gacatgaaat atataaaaca atctgtttta tgttttgcaa
    30361 ggttaaaaac accaataaaa attagaaatg gaaaggatat aaagattata cttttgttag
    30421 catataaaaa agatgatgat agaaaagtat ttagagaatt atttaaagaa atatctaatt
    30481 taacagagga tgaaaatatg ctagatattc taagtaaatg tgacattgaa aaggtaaaag
    30541 atattcttat ataaaattca tagtgaacta tggataagca caaaattttc catgtgatgg
    30601 aaaaagttgt gcatgttttt ttgtttttat aagatattat atgtttgtaa ggtataaata
    30661 atctaaaagt agagaagcaa cttaggagga aaaaatggaa gaaatagttt taaagattat
    30721 aattcatgct ggaaatgcaa aatctatgct ttatgaggca cttgactatg caaaggaaaa
    30781 cgattttaaa aaagcagatg aattaattga aaatgcaaat gaagaaattt taaaagcaca
    30841 taaagtacaa actgaactta tacaaaagga agctggggga gataaatcag atatatctat
    30901 tttattgatt cactctcaag accatttaat gacttgtatg agtgagagaa accttataaa
    30961 agaaatgatt atgttaagaa aagaaattca aaaactaaaa taaaaaatta agggggattt
    31021 aaaaaatgaa aagaaaagta tatttatttt gtagctttgg tatgtcaaca agtttacttg
    31081 cagataaaat gcagaaggta gcagatgaac acaatttacc aatagaagta gaggcatttc
    31141 cgatagcaga aattgacaaa atagttgaag aaaaaaatcc agattgtata ttattaggac
    31201 cacaagttaa atacatgcta aaggagttaa aaccaaagtt tgaagctcaa ggaaaactta
    31261 tagatataat caatgaagtt gattatggaa ctatgaatgg ggaaaaagta ttaaaacttg
    31321 ctataaagct aataaaatct aaacaagcgt agaattatag taaaaaataa taggaaatga
    31381 gggtggatag atgaatagat tggaaagaat acttatgcca ttggctgaaa aaataggtaa
    31441 aaataaatta ttaattgcaa ttagagatgg ttttttagta tcatcaccat tattaataat
    31501 aggttcatta tttttattag tagcaaattt tcctataaaa ggatggaatg aatttgtagg
    31561 tcaaattttt ggtccagatt gggctgtaaa attgcaacaa ccaactgtag caacatttga
    31621 agtaatgact ctacttgcag ttttagggat tggatattct tatgcaaagc aaatagatgt
    31681 agacccaata gcatcagcag cagttgcaat agttgccttt tttataataa caccatttgt
    31741 aataccatat acaccagaag gaacagaatc agtttacatg gtaacaggta taccactagg
    31801 ttggatgggt tctaagggaa tgtttgtagg tatgataaca gcaataacct ctgtaaaatt
    31861 atttgctgca gttgtaaaaa aaggatggac attgaaaatg ccagatggag ttcctccaac
    31921 tgtatcaaaa tcatttgcag cattaatacc aagtgcaata gttatggtag ttttcttttt
    31981 agttaagata gcttttgaag ctacaccata tggaagtata catgaattta tatttaagtt
    32041 tttacaaatg ccattattaa aattaggaaa tagtttaggt gctatggcaa tcgcttatat
    32101 attcttacat ttattctggt tctttggaat aaatggaagt agtgttgttg gggctgtgta
    32161 taatccaata ttaaaaattc tatcagctga aaacttagct gcttttcaag ctggttcaaa
    32221 aataccaaac ataataacag gtcaattcca agatatgttt gcgacatttg gtggagcagg
    32281 tagtacacta tcattagtca tagctatgtt attaatatgt aaaagtaaac gtataaagtc
    32341 aatgggaaaa ctatcaattt taccaggaat atttggtata aatgagccat tgatatttgg
    32401 tctaccaata atgcttaacc caatactatt gataccattt gcaatagttc caacaataaa
    32461 tataataata gcttacttct gtatgtcagc aggattagtg cctcttacaa atggagttca
    32521 gttaccgtgg acaactccaa ttatattctc tggtttttta acaacaggat ggcaaggtgc
    32581 agtcttacaa ttagtacttc ttatactagg aatatttatg tatataccat ttataaaaat
    32641 gctagataag caatacttga gagaagaaaa tgaaactgtt gaagaggaag atgatgatat
    32701 atcttttgat gatttagtat tataggagga agattatgaa gaaaatagtt atgatattgg
    32761 accaaataca agctggagct ggaggaaaag agaaaagtaa tatacctcct gctggaaaat
    32821 caagcccatt aggaccaggt gtaatgatgg agcaattttt aaatgaatct aaggtaatag
    32881 ctaccttatt ttgtggggat gaattttttg taaataatca agaagaagta acgagtaaga
    32941 tgatagctat ggtaaaaaaa ttaaaccctg atgtagtaat ctgtggacca agctttaatt
    33001 acgaaaactt ttctaagatg tcagcaatac tgtctaaaaa tataaatgat aaaacagata
    33061 taccagcttt tgcagctatg tcagaagaaa atatagatgt gataaacgaa tataaaaatg
    33121 acatatgtat agttaagact ccaaaaaaag gtggaattgg tcttaatgat tcgttaaata
    33181 acatatgtaa attggcaaaa gcaatagcaa ataaagaaga tataactttg atgaaagaag
    33241 aattttgtta ttaataagag tattaattat tagcaagttt aaatattata ctatttataa
    33301 taagcataca ttagtaaaat ctatataatt taaggagggg aattttaaca tgtggggaat
    33361 gatagcgaca tggagaatgg ccctagaagg aataactgag gcatcagaac atttagaaaa
    33421 aggcatggaa gcaggtgatg ctgtagagct tgctgtaaga agagttgaag actatccatt
    33481 ctataaatcg gttggatatg gtggacttcc aaatgaaaat tgtgaggtag aacttgacgc
    33541 tgcatttatg gatggtaaca cattagatat tggcgcagtt gctggtataa gagattattc
    33601 taatcctgtc agcatagcta ggaagttaag ttatgagaag gtaaataatt ttttagttgg
    33661 aataggtgct gaagattatg ctcataaaaa tggatttgaa agaaaaaata tgttgactga
    33721 tagagcgaag ttacattata aaaagagaaa aaaagagact ttagacaaag gattaagccc
    33781 atatgctggt catgatactg ttggtatgat atctttagac aaaactggaa aaatgtgtgc
    33841 agctacatca acaagtggat tgtttatgaa gaaaagaggt agaataggag attctgcact
    33901 atctggttca ggattctatg ttgatagtag tataggagga gctacagcta ctggtttggg
    33961 agaagattta atgaagggat gtatatctta tgagatagtt agacttatga aagaagggaa
    34021 gcatccacaa gaagcatgtg atattgcagt aaatgaactt gataaacagc ttatcaaaag
    34081 aagaggaaaa gctggtgatt tatcattagt tgccatgaat aataaaggtg aatggggagt
    34141 agctacaaat atagctgatt ttactttttc agtagtaact aaagaagaat ctcctactgt
    34201 atatatttca aatagagtag atggaaagac tatacataag gttatatcaa atgagtggat
    34261 ggaagcatat gaggctagaa taaaagcacc aatagaagta agtgagtaaa gtagtggtta
    34321 cataattaaa atagagtttt ataataagga gatttttatg aatatcataa gtaataaaat
    34381 agatgagtta aaagaagatt tattaagtga tattatagat attgtaaaaa tacctagtgt
    34441 caaaggggag tcagaaaatg gcttcccttt tggcgaaaaa gtaggggagg cacttaataa
    34501 agcattagaa atatcagaaa aattgggatt taaggttaga aacttggata attatattgg
    34561 ttatgcagag catggagata gtgatgatta tgtttgtgta attggacatg tagatgttgt
    34621 acatgaagga gatggttgga agcatcagcc ttataaggga gaagaaacta atggaagaat
    34681 ttatggacga ggagtactag acaataaagg acctataatg tctgccttat atggattata
    34741 tgctattaaa gaattaaact taaaattaga taagagagtt agaataatat ttggtacaaa
    34801 tgaagaaagt ggatttgaag atataccata ttatttagaa aaagaaaaag cacctattat
    34861 gggatttaca ccagattgta agtatcctgt tgtatatggt gaaaaaggca tggcaaagat
    34921 tagaataaaa agtaaaatca attatgaaga agatgtatac ctaggtttta tagaaaatat
    34981 gtctgaaaat gtgcttgtta catataaaga gctgaatata gaaaatagtg atactatatt
    35041 agatataaag gtaaaatacg attttagtta taaattgaaa gatgtattag atgaaataaa
    35101 agctagtttt ccaaatagta tcgatataga ggtaatttct aattttaatc ctgtatattt
    35161 tgataaagag agtaatcttg ttaaaaagct tcagctagct tatgaaaggg ttacatcatt
    35221 agatggaact ccagtaacaa ctaatggagg tacgtatgca aaagtaatgc caaatatagt
    35281 tccgtttgga ccttcatttc caggacaaaa aggaatagct cataatcctg atgaatacat
    35341 ggatatagaa gatataatat taaatgcaaa gatatttgca aatgcaatat atgaattagc
    35401 aaaggagtag ttatgttaga aataatagga atgtcagtag aagacgctaa aattatagaa
    35461 gattgtggtg cagatagaat agagttagtt agtgctttaa ctgaaggtgg attgacacca
    35521 agttttggtc ttatagaaag tgttgttaac agtgtaaaga tacctgtgaa cgtgatgata
    35581 agacatcatg cgaaaagttt tgtatacagt aaagaagata taagtataat gcaaaaagat
    35641 ataagtgtgg ttaaagagat tggtgctaat ggagttgtat ttggtgtatt ggataaaaac
    35701 aatagtatag atgagaaaaa tctaaatgtt ttattgaaat gttgtgataa tcttgatgtt
    35761 acatttcata gagcaataga tgaatcaaat acaatagata gtgttaaaat tttgaaggat
    35821 tatgataaaa ttacgaatat attaacatca ggagggaaag gaagtatagt tgataacata
    35881 caaatgataa agaatatgat gcttagttct aatcacataa aaattctttt aggtggaggt
    35941 cttaatttta ataatataga aaagataaag gaattgacta aggcaagtaa ttttcatttt
    36001 ggtacagcga ttagaataaa taacagtcct tttgaagata tagatagaca aaagcttaaa
    36061 caactcgtaa atataatttc aagatagtat atgaatataa aatacttagt ttaaataaag
    36121 agagtaagtt aattataatt tgggtatatg aatgttaata ggtattaaat atgaaattat
    36181 tgttgagtat gctttataac ttttaagtag ttttaaatat aaataatgaa agaaaagata
    36241 gattatggag gttttagagg gtttatattt attaaaaaat tataaaggta tattggcata
    36301 taatacacat aatttcctaa aattaccatc ttatgacatg aattcacata ttaataaaca
    36361 attaatggag atttttgtgg atagaaatag gagtaaaaat agctttaaat aaacaaaaac
    36421 aagagttatt aacagtttta tccacattat ccacaggtgt ttgtaataat attaatccac
    36481 ataagtatca atgtaactaa aaatgataaa taattctatc aattgaatag aaaaaatcat
    36541 aaaagtaaat aataaaaaga ggattatgag agttcgtgta gaatatatta ttaaagtata
    36601 aattttctta tactggcata attgaaggga gatgtattta tgaacataat tatatctgga
    36661 aaacaaatcg aattaactga tgcaataaaa aataaaatcg aaactaaact aagtaagtta
    36721 gataattaca ttcatccaga cactgatgta aaagttacag taagtgcgag aaaagcaaga
    36781 caaaaaatag aagtaacaat agctactgta aatggtccta taattagagc agaagatatg
    36841 caggaaaatt tatacacagc tatagatgtt gtatatgata aattaagtaa acaattaaaa
    36901 aaatataaaa agagattaca agataaacat aaagacaaca agagtataag attccaagat
    36961 gcagagataa atctagctga tgaacaagag gattatgggg atgaaaatca agaattagta
    37021 attcaaagac gtaagaaatt tagtgttaag ccaatgagtg aagaagaagc tgtattacag
    37081 atggaattaa taggacatga tttttatatg ttcaagaaca tagattcaga tgaaatagca
    37141 gtagtttata agcgtaataa tggtggatat ggtataatag aacatgaata aagcaattta
    37201 gatttaatca tattagctat cgataaataa attatcgata gctttttaaa ttaatattaa
    37261 aagtatagtt tttatggtaa aatgaataat agtacaataa agaacgagga gagatgtaaa
    37321 atatgagctt tatggataat ttattcaaca tggcagataa aaaagaatta aaaaagttca
    37381 ataaaactgt tgatataata gattcattag aacctaaatt tgaatctatg gcagactcgg
    37441 aacttaaaaa tatgacaaat atatttaaag aaagattagc aaatggtgaa agtatagatg
    37501 atatacttcc agaagctttt gcagtagtta gagaagtttc taagagagtg ttaggactta
    37561 gacactacag agttcaaatg attggaggta tagtactaca tcaaggaaga atagcagaaa
    37621 tgaaaacagg tgaaggtaaa actttggttg caactgctcc tgtttatttg aatgcgctta
    37681 ctggaaaagg tgtacatgtt gtaacagtaa atgattacct tgcaaaacgt gatagagacc
    37741 aaatggcaaa aatctatgag tttttaggaa tgagtgtagg cgttattata cacggtcaaa
    37801 atccaaaagt tagaaaagaa caatatgatt gtgatataac ttatggtaca aataatgaat
    37861 atggatttga ctatctaaag gacaatatgg taatacacaa agagcaaaga gttcaaagag
    37921 gattaaatta tgctatagtg gatgaggttg actctatact aatagatgag gctagaacac
    37981 cacttatcat atctggacct ggagacaaat caactcactt atattcagat gcaaacacat
    38041 ttgttttaac cttaaaacca gatgattatg aattagaaga aaaagataag gctgtatctc
    38101 taacagcatc gggtatacaa aaagcagaag tgtattttaa tgttgataat ataacagata
    38161 tatctcatac agaattatac catcatataa atcaagcttt aagagcacat gtaataatga
    38221 agaaagatgt tgattatgtt gctaaagatg gagaaatagt aatagtagat gagttcacag
    38281 gtagacttat gtttggtaga agatattcag aaggtttaca tcaagcaata gaggctaaag
    38341 aaggtcttaa aatacaaaga gaatctaaaa cactagctac tgtaacattc caaaattact
    38401 ttagaatgta caaaaaactt tctgggatga ctggtacagc aaaaacagaa gaagaagagt
    38461 ttaaagcaat ctacaaaatg gatgttttcc aagtacctac taataagctt atgataaggg
    38521 aagacttacc agactgtgta tacaaaagtg aaataggtaa atttaatgct gtagcacaag
    38581 aaataattga aaggcacaaa gtcaatcaac caatacttgt aggtacagta tcaatagaaa
    38641 aatcagaatt attatcacaa atactaaaga aaaagggcat aaagcacgag gtattaaatg
    38701 ctaagcatca tgataaagag gcagaaataa tagcacaagc tggtagactt ggagctgtta
    38761 caatagctac taatatggct ggtcgtggta cagatatagt tctaggtgga aatccagact
    38821 ttttaactaa gagagaaatg agaagaaatg gatttaaaga agaaatagta aatagagtag
    38881 atactccaat agaaggtata cctgtaaaag gtaatgaaat tctatttgaa gctagagaag
    38941 agtatgaaaa actatttgaa aagtttaagc aacaaacaca agaagaacaa aaacaagttg
    39001 tagaagcagg tggacttgct ataataggta cagaaagaca cgaatctcga agaatagata
    39061 accagttgag aggtcgtgct ggacgtcaag gtgacccagg gagttcaaga ttctatatag
    39121 gtcttgatga tgaccttatg agattgtttg gtagtgatag aatatctggt attgtagata
    39181 aaataggtct tgaagaagac atgccgatag aacatagaat attatctaag tctattgaag
    39241 gagcacaaaa gaaagtcgaa ggtaaaaact ttggaataag aaagcatgta cttcaatatg
    39301 atgatgttat gaataaacaa agagaaataa tatatgcaga gagaaaacgt gtattagaag
    39361 gtgaagattt acaagaacaa atacaatcaa tgactcattc tataatagaa gaagcagtta
    39421 ctctttatac acaagataaa ggatttgatg aagaaggatt caaagaacat atgtataatc
    39481 tgttcttgcc aaaaggtagc atagaaatac cagaaataga aaaacttaat ccagttgaaa
    39541 taacagaaaa agtttatgaa atagctatga agatatatac ttcaaaagaa gaacaagttg
    39601 gctatgagag aatgagagaa gttgagagag taatcttact tcaagctgtt gataaccatt
    39661 ggatagacca tatagatgct atggaccaat tacgccaagg tattggtctt cgtgctgtag
    39721 gtcagcaaga cccagttatt gcttataaaa tggaaggttt tgatatgttt gatgaaatga
    39781 ataaacatat caaagaagat actgtaagat atttatttaa tataacaata gaaactccag
    39841 tggaaagaaa agcagttgta gatgttgaaa atctatcttc tccatcagat ggaacacttc
    39901 caacaagtaa aactgttaaa aaagatgaaa aggttggaag aaatgactta tgcccttgtg
    39961 gaagtggtaa aaaatataaa aactgttgtg gaagataatc tcggaggatt aaatgttaaa
    40021 gttacaagag tatagacata gtatggagca gatggcttca aatttagaag aattgagggt
    40081 ttctctttga catagcttca ttgatgaaaa atatacaaat taatgaagaa aagatgaatc
    40141 aacaagattt ttggaatgat aatgaagtcg ctcaaagagt acttcaagaa aataaatctt
    40201 taaaagagac attagaagaa tatgaaagct taaaaagctt attagaagat atagaagtat
    40261 taattgaaat aggtttagaa gaagatgatg attctgtaga aagagatata gaaaagtcca
    40321 tagaatcaat ggaagaaaaa ttatctgaaa tgaaaataaa gactcttcta aatggagagt
    40381 atgataaaaa taatgctata ttatctataa atgctggtac tggtggctta gatgctcaag
    40441 attgggctca aatgcttttg agaatgtata taagatggtc agaaagtaag ggttataaag
    40501 ttaagctttt agatataata tcagaccctg aagcagggat aaagacagct acaattcttg
    40561 tagaaggaac aaatgcttat gggtatttaa agagtgaaaa aggagttcat agattagtga
    40621 gaatatctcc atttgaccca tcaggaaaaa gacatacttc atttgcatct atagatgtaa
    40681 caccagagtt agatgaaaat atagaagtag aaattaatcc atcagattta aagatagaca
    40741 catatagagc ttctggagct ggagggcaac atgtaaatac gactgattca gcagttagaa
    40801 taacacatat ccctactggt gtagtagttc agtgccaaaa tgaaaggtct cagcatctaa
    40861 ataaggacag agctatgaga cttttaatgg cgaaattgat agaattaaaa gagttagagc
    40921 aaaaggaaaa aatagaagat atacaaggca aatactcaca aataacttgg ggtagtcaaa
    40981 ttaggtcata tgtttttcaa ccatataaat tggttaaaga ccatagaaca aatgctgagt
    41041 ttggcaatgt agatagtgta atgaatggaa atatagactt gtttattaat gaatacttaa
    41101 aaatgaataa aatagtttaa taaatatgca ctgtaattaa gatatatgta taagaaggtt
    41161 agtgcttaat atgcacttac agcacttagg ctgaggacga taaccctcag ccttcgttct
    41221 gttttgttat acttatgaaa aactaacatt aggagtgaat aaattggata taaatcaaat
    41281 cttaaaaaaa gagtttaatc ttagagatga acaaataaat aatacattaa aacttattga
    41341 tgaaggaaat acaataccat ttatagctag atatagaaaa gaaatgactg gcgaaatgag
    41401 tgatgttaca ctaagagaat tttatgaaaa attaatgtat ttgagaaatc ttcaaagtag
    41461 aaaagatgat gtagttagac taatagatga acaagggaaa ttaacagatg aaataacaca
    41521 aaatatagaa aaagcaaaaa cattacaaga agtagaagat atatatgctc catacaaaca
    41581 aaagaaaaga acgagagcaa caatagcaaa agaaaaagga ttagaaaatt tagcattgtc
    41641 aatactagag aataatttag ataatataga aatagagtca aagaattatc ttgatgaaga
    41701 gaaagaagtt ctttctattg aagatgctct aaaaggtgct agagatataa ttgcagaact
    41761 agtctcagat gatgctaaga ttagaaagta tataagagaa ttagctttaa gagaaggtat
    41821 gatagttagc aaaagtgcta ctgatgagaa atcggtttat gatatgtatt atgattatag
    41881 cgaagcagta aaaagtatgg ctccacatag agttttggca ataaacagag gagaaaaaga
    41941 aagcttccta aaggttaagt tggaaataaa taatgataaa gttttaaatt acataataaa
    42001 cgaatatgta aatgataaaa atttcaaaaa taaagaagaa atcgtaagtt caatagaaga
    42061 ttcttataaa agattaattt tcccatcaat agaaagagaa ataagaaatc atttaacaga
    42121 aatagctcaa gaaagagcta taagtgtatt tggaaagaat gttaaaagtt tattgcttca
    42181 accaccagtt aaagacaaag tagttatggg atttgaccct gcatttagaa ctggatgtaa
    42241 aatagctgta gtagataaaa atggtaaact actagattat acaacagtat atccaacaga
    42301 ccctcaaaat gatgtagagg gagcaaagaa agttttaaaa ggattgattg aaaaatacga
    42361 tatagatata atttcaattg gaaatggtac tgcatctaga gaatcagaaa cttttgtatc
    42421 agaaatgata aaagaaatag atagcgaagt gcagtatgtg atagttagtg aagcaggagc
    42481 atctgtttat tcagcatctg aacttgcaaa tgaggaacat ccagatataa atgtatcaat
    42541 aagaggagct atatctatag ctagaagatt acaagaccca ttagcagagc ttgtaaaaat
    42601 agaccctaaa agtattggtg ttggtcaata tcagcatgat ttaaacaaaa agagattaga
    42661 agaagtacta gatggagttg ttgaagattc tgtaaatagt gttggagtag atttaaatac
    42721 tgcatcatac tccctactag aacatgttgc aggaataagt aaagctatag caaaaaatat
    42781 aatagcatat agagaagaaa atggagattt tacgtcaaga gcacaattga aaaaagtaaa
    42841 aagactagga cctcaagcat ttactcaatg tgctggattt atgagaatct tagagggtaa
    42901 aaatccacta gataatacag gtgtgcatcc tgaaagttat gatatatgta aaaaaatgat
    42961 tgagataatt ggatattctt tagatgatgt aaagaataag aatataggtg aaatagatga
    43021 aaaaataaaa gaaattggct tgagggaatt aagtgaaaaa ctggaagtag gacaagttac
    43081 attaaaagat atcatagctg aaatcaaaaa accaggaaga gacccaagag aagaaggtat
    43141 aaaacctata cttagaactg atgtcttaaa aattgaagat atacaagagg gtatgacttt
    43201 aaaaggtaca ataagaaatg ttgtagactt tggtgcattt gtagatattg gaataaaaaa
    43261 tgacggatta gttcataaat cagaaatgag taatagtttt gtgaaagacc ctatgagtat
    43321 tgttacagta ggtgatatag tagatgtaaa agtcattgga attgacttaa acaaaaaaag
    43381 agttgcctta tcaatgaaaa aatagataag tttatgtatg gttataatac ttagattcta
    43441 tatttgtaaa taagattact cgagtgtata aatgttaaaa atttgtaaaa gtatatagta
    43501 agaaaatata ttaaaataag aacgatagtc ttgaaaatca ggaggaaaat gaatgatttt
    43561 tataaaaaat ggtaaaataa ataccataac aaatgggata attcatggag atatacttat
    43621 agatgaagga aaaataatag aaattggaga agatttgata gctcctttag atgtagaggt
    43681 aatagatgct agtaataaac ttgtgtttcc tggatttata gatgctcata ctcacttagg
    43741 tttatgggaa gatggtatag ggtttgaagg agcagatgga aatgaagaga ctgacccaat
    43801 aacaccacaa ttaaatccta tagatggaat taatccaatg gatagaactt ttaaagaagc
    43861 ttttgaaggt ggaataactt ctgtttgtac aacccctgga agtgcaaatg ttatgggtgg
    43921 tcaatgtata gctataaaga catgtggaaa aagaattgat aaaatggtaa taaaaaatcc
    43981 agttgcatct aaaattgctt ttggagaaaa tccaaagagc tgttatggtc aggatgataa
    44041 atctcctcaa actagaatgg ctatagctgc tttacttaga gaaaacctaa aaaaagcaga
    44101 agaatattta gaggatattg atatgtatga gagtcatgat gatgaagact gtgaaaaacc
    44161 tgaatatgat ataaaaatgg aaagtttaat accagtttta agaagagaaa ttccttttaa
    44221 ggcacatgct catagagcag atgatatgtt tacagcaata agaattgcaa aagaatttaa
    44281 tttaaagctt acattagacc attgtacaga aggtcatctt attgtagatg aattagtgga
    44341 agaagggttt cctgtaatag tagggccatc tttatcagaa aggtctaaat ttgagcttag
    44401 aaatttgaca tttaatacag caggaatact ttcaaatgca ggtcttgatg tatgtataat
    44461 gacagaccat ccagtaatac cagttcaata tcttccaata tgtgcaggta ttgcagtaaa
    44521 acatggtatg aaagaagaaa aagcaataga atcaattacg ataaatccag ctaagacttt
    44581 aggaatagaa gatagagtag gctctataga agttggtaaa gatgcagacc tagtaatttg
    44641 ggataattct ccactagaga ttcaaagtaa tgtactttac actataataa atggcaaagt
    44701 agtgtatgag aaaaaataac ttgacaatta ttaaaaaatt aactaatata agagtgtaag
    44761 gaaaattaaa aaatcaaaga agatcttcgg ggcagggtga aattccctac cggcggtata
    44821 gcccgcgaac tacttaagtg gttgatttgg tgagattcca aagccgacag taaagtctgg
    44881 atgagagaag atatttatgg aagataaata agtttatttt agtttgttta aactatatcc
    44941 ataaaaactc gaagataggg tcttcgagtt ttttgttttt cctaaaaaat ataaacatat
    45001 tgattagggg gaaaataatt atgcaaaaca cagcaaaaag aggcaatatg atttcaacaa
    45061 gaactttagt agtaatgtca attttatctg caatatctta tttattaatg tttattcaag
    45121 caccaatacc aggtatattt ccagatttct taaaaattga cttatcagat attccagcaa
    45181 tattcggagg aatgtctcta ggaccattag ttggatttgg aatagttgta gtaaaaaatg
    45241 tgttacaagc aataacagca acaacaactg gaggaatagg agaatttgcg aatatattaa
    45301 ttggtggttc ttatgtaatg atactttgtt attcatataa gagaagtgga gatttaaaag
    45361 gtgtactgat tggttttgta ttaggaacaa tatctatgac tataattggt tcattagtaa
    45421 attattttgt aatgttgcca ctttatggtc aacttatggg actagatgct ataataggtt
    45481 taggttcagc tataaatcct caagttcatg atttattcac ttttgtaatt tggatgatag
    45541 ctccatttaa tgtgctgaag gctgttataa tatctttagt aacattgcca ctttataaaa
    45601 aaatgggaaa tataataaag aaataattag acaagaaaat ataaaattaa atgtattgaa
    45661 tagccataat ttatttataa cttcagtcct atattgtgat tgatatatta aatatattat
    45721 ggctttatta atttatcaaa aaatgtatat aaaataagaa atatttagac aaatttaaat
    45781 tttaaaataa aggaagaatt aaaacgatta actatt
//
LOCUS       DAKBNN010000009        42172 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000009 DAKBNN010000000
VERSION     DAKBNN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.009"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1438
                     /locus_tag="OUB02_000518"
     CDS             <1..1438
                     /locus_tag="OUB02_000518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884317.1"
                     /translation="TLIDEVNTVKTEVITAKNNMISEVTTAKETMQTEVTDAINAIPA
                     KEELKGVGIEIKGSLDNISALPTNPTLSDAYFVKSATIENQIDLYVWDNTSWIKVPDI
                     KIKGENGDNLEFNWNSTQLGVRVEGQTDYSYVDLKGQKGDKGDSIEFNWNGTQLGVKI
                     EGQENYSYTELKGEQGYTPTIGENGNWWINNIDTKKPARGASLRILGKLDSIDNLPLD
                     PTIGDCWIIGRNIYIYQTKWEDLGSLAGVDGKNLEFNWDGTQLGVRQQYELDYKYIDL
                     KGDNIEFNWDGTQLGVRIEGQENYTYTDLKGQKGDSIEFNWDGTQLGVRIEGQEDYSY
                     TNLKGATGNKLEFNWSGSQLGIREEGQTEYIYTELRGEQGYTPAIGENGNWFINGEDT
                     GKASKGKVTWNELLEKPKELDYIKKSTTFNSDGSITDILDSVSKTITKFNADGTIVDE
                     KYIDNVLVSKVKTTFKGNQIEEIKEEVS"
     gene            1438..3321
                     /locus_tag="OUB02_000519"
     CDS             1438..3321
                     /locus_tag="OUB02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT8884318.1"
                     /translation="MSWAEIYKVNSDLQGEPLNFLSYLQDIKLNGLDSYVLFIGNARI
                     WEELYLNSLYLFSDRGIRETVYTAFSETDIDNLFNKSTKLGEQLNAFYRTDIFSLGNA
                     DNVVKGMTMEHYNSLEEKFKAGYDRYVTREQEKSTIGAWFNSTFSLDNTDLENLTTIE
                     EILANVEATNAILNNSNAIVALTMCKSSMDAVVASSNAMDLLGQYILRITTESPVIRA
                     ILKNNVIRDAIINSDEAMTQISSNENSVMEIFNDLEATKVLVQNQNSINKILTNNVTV
                     EKIIPNLLEMKYNLQTSLNYINTIKSNIASGKGQIMAITYNEEIFPILKNAAKNYDGM
                     ENTRNILQRDIEEKIKISDAILESSIAMTTFVNNSIIVNKVGDRAGIIESILSQTVSL
                     NAFMKSTTAIKVLVNKATAFTKIVNNSTAFNAMLTISGNISSVANSSTAMNIIANNAQ
                     AMATVARNDTVLTVFINASTAMTAIAGNVTSMSAIVGYSGAMNKITTSAFALNRIFKS
                     TIGKDILISNNAILQTYKNGIHNTLKSSSGQFRLITSPTDSDSAPTQIINSSYVGVTY
                     CYGYRGGTGKFAIVYHGHKTSAEAGRGDGDKDETKKFITLGGTKFVESGDGYFNYSIY
                     QAI"
     gene            3408..3671
                     /locus_tag="OUB02_000520"
     CDS             3408..3671
                     /locus_tag="OUB02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884319.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKDMLQKEGL"
     gene            3672..4478
                     /locus_tag="OUB02_000521"
     CDS             3672..4478
                     /locus_tag="OUB02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8884320.1"
                     /translation="MKVALTAGHTLTGKGTGATGYINEGTENRILMDLVVKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNADHTTLNKMGTETIYKTNNGKVY
                     AERVNEKLATIFKNRGAKSDARGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGRIMYKHTIVYDGAVDKIPATVVGWGYNDGKILICDIK
                     DYIPGQTENLYVVGGGACEKIGSMTKENYTMIKGNDRFDTLYKALDFIKK"
     gene            complement(4637..4894)
                     /locus_tag="OUB02_000522"
     CDS             complement(4637..4894)
                     /locus_tag="OUB02_000522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884321.1"
                     /translation="MKNITEQLKETIVNELYDIETNEGCHEDYIEDYETELDFYLSNV
                     KFGTYEVYVKEYCSNNYDISISDELAFEIMDDLIGKIKDNN"
     gene            complement(4940..5377)
                     /locus_tag="OUB02_000523"
     CDS             complement(4940..5377)
                     /locus_tag="OUB02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884322.1"
                     /translation="MNAKLNKELYNLGFKIGSENVLSRNKILELNDAIYRYQDRNKAF
                     EIILKAFMKLDIPMPAEIIENLDNYEVIVNFVVGVYNGYIEQLNNFSNFISLSDASKK
                     YNKAESTLKQNIKNGKFVEGVDCRLFGKSWVFNIDSLEREYSK"
     gene            5549..5845
                     /locus_tag="OUB02_000524"
     CDS             5549..5845
                     /locus_tag="OUB02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884323.1"
                     /translation="MKKITKKEIIEFVRDVVGEYQDWKLKSCGFYIKDNELNSFVSFE
                     GKGIDINVYKENYDEIIYIEDYIKDYKRKEYNLKEIDSIIYEDVNEMISNYNEK"
     gene            5886..6356
                     /locus_tag="OUB02_000525"
     CDS             5886..6356
                     /locus_tag="OUB02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884324.1"
                     /translation="MNKQKARRFLRVIDMNIDKIEEEAIKAFKESCLIKETNNIKIYI
                     DIQGKVEAIAVQTWAKLLDDNKEINIFTLNQTPTHLNDLFGEICYVNDYEEFENWCEN
                     EWENLDWDSYKKFNKENFEEIAERNIDDSTSVFLEELQKSIESCKQELQNVIEN"
     gene            complement(6589..6870)
                     /locus_tag="OUB02_000526"
     CDS             complement(6589..6870)
                     /locus_tag="OUB02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884325.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVTNYLRFAGHDNDMKIKL
                     FQTIIEAIARKRVTLPADNEFTQGIMLSNELEMTTNVGFIIEGLSYHNN"
     gene            complement(7014..7349)
                     /locus_tag="OUB02_000527"
     CDS             complement(7014..7349)
                     /locus_tag="OUB02_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884326.1"
                     /translation="MPSFNEDLLSNKEKPVFKKTGTIVQNGSTTTDNKTLNIYNLVNK
                     KSKKVTMSATLNEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNER
                     NRRNKTKKK"
     gene            complement(7350..8162)
                     /locus_tag="OUB02_000528"
     CDS             complement(7350..8162)
                     /locus_tag="OUB02_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCT8884327.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYKVNHNDKTTLDILKNCSADDIIIKSFNKKYSNVDLIASDIQMTLLEENL
                     FNFKDDKEKILAKWYMENFDTFSKYDYIICDLSPHYDLIAKNVLFLADSIIVLIQDKN
                     VSSLRGAELFKQLWDADRALLDKEDNIKNFVLVGFEKKKTQVSEVFDSYLEGFSELRS
                     MMLKTYIRKNEFIEKALMKKLSLTDFLKDSKDHFSRQEFTNMLEELKAKGVL"
     gene            complement(8448..8630)
                     /locus_tag="OUB02_000529"
     CDS             complement(8448..8630)
                     /locus_tag="OUB02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCT8884328.1"
                     /translation="MEKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNK"
     gene            complement(9243..9380)
                     /locus_tag="OUB02_000530"
     CDS             complement(9243..9380)
                     /locus_tag="OUB02_000530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884329.1"
                     /translation="MVINFLLSILAGVISAFIYDKIKNHSNANKSGWEFDLKIKFKKF
                     K"
     gene            9895..12060
                     /locus_tag="OUB02_000531"
     CDS             9895..12060
                     /locus_tag="OUB02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCT8884330.1"
                     /translation="MENNNKNNIDKMFLEECSAERAEHEKENYSNFNRHETYEIKKYI
                     NEKKLGIKTAIANAINGGKTLFIAPTGAGKSYSFINTLKKLKTKALFILPNASNVEQA
                     MHEYDIPGAYDKIPVSEAFKSGNLVVMTWDKTEKLINTDLSEYIIVVDEIHQTYTDAY
                     RGKAIKNLNNIMSKCKGRIDITATPTKLEFEIYDYIIEYTQKQKTEYKVKLYNDFDNK
                     NFTEMINIINKSKNSAMLMNDISTLEFIRDSVNKKVGVVYADGKEENELYNRIVKNSD
                     MKDYETLLNTTTILAGVNINNKDITDIIIVNIKDVGAIKQYVARFRNLKKVKVHIFNK
                     YEEECNVYKIEWLVNKNIEKATILKDAYNKVSKHMLQFETVGINATPIRIDSNVYYCT
                     KDNCYKVDKLYIKSQVYSNYYNTRTIQSFKVLLEEYFENVNITDAKEVGTNEKELKEY
                     KKVVKEIKDATREILKEHKEILVGYRQIKSDSKSFSLMQYHNDMKLSSKGCLEAYRAY
                     DIHNLVKKSKSNSMLELYSNYVLDNKFDLDLAWKLANLSNRKRGAVFNKINTLIYREL
                     KEEYPEFLNDELIQVTVFNFIDKLFGIGTSYTELHLQELSNDLRTFLGENWGLNTKKI
                     GCILNEIFVIEKKKYRSVNHLENIFFYKNMNPKWFTVEVKQIQLSNIKRYVTVDDIKK
                     DLELDEKDKSLERAIKHTKDRMLNSLDEDEKLLLLKGLM"
     gene            12084..12308
                     /locus_tag="OUB02_000532"
                     /pseudo
     CDS             12084..12308
                     /locus_tag="OUB02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            12329..12667
                     /locus_tag="OUB02_000533"
     CDS             12329..12667
                     /locus_tag="OUB02_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884331.1"
                     /translation="MKLRIGIIDKYVAEASYELSELLIDAFNKYDEFVEAIRGGEIRI
                     VPFNLGFQALLEEDSTYDEYEFYYDDEDDKCFFHIKYRNVKDVELSKELLLELIKVNL
                     PAGAKLIVNN"
     gene            13000..13266
                     /locus_tag="OUB02_000534"
     CDS             13000..13266
                     /locus_tag="OUB02_000534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884332.1"
                     /translation="MKEEIKEMVDEIRDRVKSLGKQFQVLLINISKYQGVIMKKSQIK
                     TKNRYIENKIETAICVNELKYISVDDYVFLKECIEKIYIKNKRT"
     gene            complement(13301..13669)
                     /locus_tag="OUB02_000535"
     CDS             complement(13301..13669)
                     /locus_tag="OUB02_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884333.1"
                     /translation="MDEISKETINKEIGKRLNLLRTNEKLSQKAFGEKIFLSQDQISL
                     LEKGKRVLTERSINDICREFDVNEEWLRKGIGDIYKDCLSELEIDEDVKEITNKLYEL
                     EHEDRNAILQMIELLHKKNN"
     gene            13838..14014
                     /locus_tag="OUB02_000536"
     CDS             13838..14014
                     /locus_tag="OUB02_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884334.1"
                     /translation="MVCSSKEKIETALMIEFLKKNDPRNWESIETLISSTYTLSNFKK
                     RNKISQKRGDKIEL"
     gene            13995..14210
                     /locus_tag="OUB02_000537"
     CDS             13995..14210
                     /locus_tag="OUB02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884335.1"
                     /translation="MIKLNFNKYRKCEIVQLYETKFNCNRNCIVTYADEKELVLMYYD
                     KEIEELIYKTLTKEDLIFNDYELKLLS"
     gene            14333..15115
                     /locus_tag="OUB02_000538"
     CDS             14333..15115
                     /locus_tag="OUB02_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HCT8884336.1"
                     /translation="MIYITLVRHLNENSFCFPSYKTISKKSGCSESQVKKVIKSLENK
                     ELIKKENRNKLNSKEKDSNIYFVLTAKISRACSDLPVGHSETHPRACGDLPVGHVVSS
                     NKTNINKTNLTNNIYSSAEQKEEKAKDKEIYKRIVDYLNKTADKSFKSTTKKTISLID
                     ARLKEGFTEEEFYKVIDNKVLNWLDNDKMNAYLRPETLFGNKFESYLNETPKKALKGN
                     ESVKGKTKTNNNKFANFNQTFDKYSEDELDAIIKKSQSAKFK"
     gene            15140..15586
                     /locus_tag="OUB02_000539"
     CDS             15140..15586
                     /locus_tag="OUB02_000539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884337.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKK"
     gene            15787..16068
                     /locus_tag="OUB02_000540"
     CDS             15787..16068
                     /locus_tag="OUB02_000540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884338.1"
                     /translation="MIKNNVLSYEEKIKVLKKHGYSDFTKEYVLKEFDKEHAFYKSFL
                     RAIEKEDDGKGVLIKKIGKGQHQFSKGFIKTHTISELLEEAKRIRKILE"
     gene            complement(16106..16450)
                     /locus_tag="OUB02_000541"
     CDS             complement(16106..16450)
                     /locus_tag="OUB02_000541"
                     /inference="COORDINATES: protein motif:HMM:NF022172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCT8884339.1"
                     /translation="MVTNQDCIRDILLLIESNTTPISPVLQFDTILDNLTYDKSTIVH
                     HINLIYQNNLVDDVNYGDDTVLEVMQLSLKGYQYLDKIRDNTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            16539..16712
                     /locus_tag="OUB02_000542"
     CDS             16539..16712
                     /locus_tag="OUB02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884340.1"
                     /translation="MQKKILEMSILEKADLISKKQKKLQEKYNLSKGELFSIACNEVK
                     KIEEQEGKYFEYR"
     gene            16699..17064
                     /locus_tag="OUB02_000543"
     CDS             16699..17064
                     /locus_tag="OUB02_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884341.1"
                     /translation="MNIGSYTLVKKQLIEELKEEIKQKEENLKEREKYIKELKESLEY
                     FARDALESEECIIDIENILIEQAVELEAYNLFIDCILNKDIRKAIAIYNRTKSIRIKH
                     KNINSISKKIEKIKQIISL"
     gene            17084..17260
                     /locus_tag="OUB02_000544"
     CDS             17084..17260
                     /locus_tag="OUB02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884342.1"
                     /translation="MDKFVTCARCERTINVEENNYVKYEEETLNLSFTLYFCLGCVDK
                     LIEEQLKSMEGENE"
     gene            17253..17468
                     /locus_tag="OUB02_000545"
     CDS             17253..17468
                     /locus_tag="OUB02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884343.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYKKEYRGKFYDWIKSLE
                     RKDSLQNYKIVEVLNNERNGEEAPFWN"
     gene            17527..17700
                     /locus_tag="OUB02_000546"
     CDS             17527..17700
                     /locus_tag="OUB02_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HCT8884344.1"
                     /translation="MKMINVFKLSSMYCICPKCGSDKLGKNEGKLIVDEHTYYRECKC
                     GFNVLVDEREDEI"
     gene            17715..18119
                     /gene="ssb"
                     /locus_tag="OUB02_000547"
     CDS             17715..18119
                     /gene="ssb"
                     /locus_tag="OUB02_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT8884345.1"
                     /translation="MNSVILIGRLTKDPELKYIPGSGTVVSTFTIAVDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLGKGRLVAVEGSIRVNLYEKDGEKRTYTKVHANKIKFL
                     DYKKDDAGKEYMFEPGGLDPQGFQAIDDGIPF"
     gene            18138..18527
                     /locus_tag="OUB02_000548"
     CDS             18138..18527
                     /locus_tag="OUB02_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884346.1"
                     /translation="MNEKWKQYIENVLESRGYCINRMPYVYSYDFLVNSNIKINVEIS
                     NLYKNEENEYHYFELEKEYHSCDIYILIALDEVGSALKILVIPASNLMGQKQIIINNK
                     SKYDKFDSRFELIKVYDNFYNDLDYVI"
     gene            18539..18901
                     /locus_tag="OUB02_000549"
     CDS             18539..18901
                     /locus_tag="OUB02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884347.1"
                     /translation="MFKKVEKNFSGSVNPNFMEDDPTFKNYLALYCCIKPVKIRGNKK
                     VLASSTKACSALKIRERQKKERKRVRLVNAKTGEIKEMAIDEAENFLNVKDLYPVIRR
                     GRPTRTGWYVKDVEDEGD"
     gene            18888..19058
                     /locus_tag="OUB02_000550"
     CDS             18888..19058
                     /locus_tag="OUB02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884348.1"
                     /translation="MKETKYNVIYFNSETLKMDKRSFDALKDARAFKKEKEKKYKNVE
                     IIKKTIIEKLIM"
     gene            19070..19642
                     /locus_tag="OUB02_000551"
     CDS             19070..19642
                     /locus_tag="OUB02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT8884349.1"
                     /translation="MAKKKRICSWCGKSFFTESKNRFCCKSCERKYKKDKEKNASSCL
                     VEETKEYLIELGNKKRRVDILRKEIDFLKDKEFYREINFNELGFKIHSSPKGIDEMIM
                     NNEDKISFKESEIDFIEYRLRLMDIYISELTEEKQQIIKFMYFYDGASKLSNEEIASK
                     IKCSRSNVYYKHKEALKELAEMLFESGSLL"
     gene            19820..20428
                     /locus_tag="OUB02_000552"
     CDS             19820..20428
                     /locus_tag="OUB02_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8884350.1"
                     /translation="MGSKKPANPVKDKRMVLNIQEYLKEKSVRNYVLFVLGVATGYRA
                     GDLVKLQVRDVRNAIDEGYFLIMESKKEKTKNIRKKNKKPRKAPIVPNLERVLKSYIR
                     DKKDYEYMFPSRQKSVTPHIGVERVTVILKEAGRYFGLKHITAHSMRKTYAYTIYEES
                     GFDIIRVKEMLGHSSIEETKVYLGLNEEQYQEYSMFLNDLIG"
     gene            20893..21462
                     /locus_tag="OUB02_000553"
     CDS             20893..21462
                     /locus_tag="OUB02_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT8884351.1"
                     /translation="MIFGYCRVSSKVQIDNNSLEQQEQEIKRHYSDAKIFREQHTGST
                     THRPIFEEMVKQMRDGDKLVVTKLDRLARNATEGINLIQNLFDSGISVHVLNVGLLEN
                     TTLGKFFITTLLAVAEMERNLILERTFAGKEIAKQNPNFREGRPKKYTKMQMEHALEL
                     LKTNSYTQVERMTGISKSTLQRAKRKQED"
     gene            21491..22198
                     /locus_tag="OUB02_000554"
     CDS             21491..22198
                     /locus_tag="OUB02_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884352.1"
                     /translation="MICISIKNSNREKAFEIYKEKNGRIKLIDISKMLEEKSCNISRW
                     KKIDRWDYRLGINKKVGAPAGNQNALGHEGGAPANNQNARTHGFFSKLLPARTYEIVK
                     YIEKDGGNSLDILWNSIVVQYAKILDSFKITHVKNKKDHTTDIKKNGNKIKEYEIQHS
                     WDKVTNAMKAEATAFKALSKMIKDYEELLHKNWDLATEEQKSRIENIKARTNKLTGND
                     LEIEDIEEIEAEIYGSN"
     gene            22185..23507
                     /locus_tag="OUB02_000555"
     CDS             22185..23507
                     /locus_tag="OUB02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCT8884353.1"
                     /translation="MAVTKKKTIPFRFGDKHKEYIKKCAINTYNIAEGAVRAGKTVDN
                     VFAFAHEIKTSKDKIHLATGSTSANAKLNIGDANGFGLEYIFRGQCHWGKFKGNECLY
                     VKGISTKHKQKIVIFAGGAKADSYKKIRGNSYGMWIATEINLHHDNTIKEAFNRIIAS
                     TNRKIFWDLNPDNPNSSIYKEYIDAYMKKDREGTLLGGYNYQHFTIHDNITVSDERKR
                     AIISQYDEGSIWYKRDILGQRCVAEGLIYEYFANNKEQFKTETVDPLMDVIIGVDFGG
                     NKSYHAFVATGITYNYKKVIALASERPSADTSPDELNALLINFIKRVINLYGKVDYVY
                     CDSAEQVLIRGIKNAVEKENLNVSVRNALKSAINDRIRLTDLLISQGKFEYTRYSETL
                     VSALCGAVWDSKEINEDVRLDDGSSDIDSLDAFEYTIERYLKRFIRGD"
     gene            23508..24920
                     /locus_tag="OUB02_000556"
     CDS             23508..24920
                     /locus_tag="OUB02_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8884354.1"
                     /translation="MFDGLKNAIRGVINKLFNKSSIQNELKVDIAVSDKMSRAIDLWM
                     SMYKNKSPWINEITKSLNLSATISSEVARLVTLELESEVVGNDFLNEQYQDVLKDIRK
                     YCEYACGVGGLVFKPYVSGDKIEVDYVQASNFFPVEYSSTGDIISAIFSEIKVKGDIK
                     YTRLEYHNWTSTSYTISNYAYKSNTAKLIGYSDDLGKRIKLSDVPEWAELSEELVLEG
                     IERPLFAYFKIPQANSLENSSPLGVSVFSRAVDLIKEADKQYSRILWEYEATEIAIDA
                     DSSMFRRNANGEYEVPHGKKRLYRMLEMEDGDNKWNVFSPAIRDSSLYAGLNQLLRKI
                     EFNCGLSYGIISDAQEIEKTATEIKTSKQRLYSTVKDIQKSLEDALEGLIYSIDKWSI
                     LAGFTPSLKYETTFNWDDSVIIDKDSELLAMQQDVASGLIRPELYIMKKYGVTEEEAL
                     KMMPDTGELVEDNPFDKDGI"
     gene            24921..26144
                     /locus_tag="OUB02_000557"
     CDS             24921..26144
                     /locus_tag="OUB02_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HCT8884355.1"
                     /translation="MLTPNELQEIPKYFIGLFQSLEDFIIADIARRIAKVGNITDMAE
                     WQLIRAEEIGIADKVIKRKIAETLGISFETVDKLFEESAIKSIESDSALYEHAKLTPI
                     HLNSSEELKSYVSSAKEQTKGKLKNMTGTLGFCTIKKGRVISKKLTDIYIESLDLAQF
                     QVSTGVLDYKTAVKKAVKRLADSGLRFIDYETGWTNRIDVATRRAVLTGVNQMSQNIN
                     NKVIDDLDTDIVEVTAHSGARCEGAGIKNHKKWQGKWYSLSGKSTKYPSLRAVTGWGQ
                     GDGLGGWNCSHQFHAVVPGISVPAYTKEQLKNIDPPDIGYKGRTYTHYQALQYQRKIE
                     TAMRQTKRQLIAYESAGLKDEFTNASIKLQRQKQEYREFSKVAKLRLQNDRHQVLGYN
                     KSISQKAVYAGKKEK"
     gene            26146..26472
                     /locus_tag="OUB02_000558"
     CDS             26146..26472
                     /locus_tag="OUB02_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884356.1"
                     /translation="MDVDILGAKYTIVKDCTKDKEPLLAEFDGFMDDTVNKIIIAKME
                     HSDESLKDLNFYEKQVLRHEVIHAFLSESGLKSNSDWARNEEMVDYFAIQFPKMLKVF
                     MKLDAL"
     gene            26489..26770
                     /locus_tag="OUB02_000559"
     CDS             26489..26770
                     /locus_tag="OUB02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884357.1"
                     /translation="MGKYRKKPVVIEAFKWLGSAEQKEEPTWIVRAIESGNVWIEQSL
                     GELSPRMYIRTLEGIHEANVGDYIIQGIKGELYPCKADIFRETYEEVLY"
     gene            26902..27474
                     /locus_tag="OUB02_000560"
     CDS             26902..27474
                     /locus_tag="OUB02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884358.1"
                     /translation="MKREFLKDLNLEDEVIEKIMSENGRDIEKYKKEVEKKKEELESK
                     NTELETANNKIKDLEKIDVESIKKEANDWKSKAEQAQKDKELIENQMSEQTYNLNLDN
                     YLSNFKFSSNLSKEAVKIKMKEKGLEYKDGAFEGADDYIKELQANDPGAFISGNSVPK
                     VVSSSSGDAGESKVSLMEQMIAKNREMLNI"
     gene            27485..28558
                     /locus_tag="OUB02_000561"
     CDS             27485..28558
                     /locus_tag="OUB02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage coat protein"
                     /protein_id="HCT8884359.1"
                     /translation="MSLFDSKIFNGEVFGKYVETVPNLNRDELIKSGAIRRRGDLKAM
                     FSAQSGANYATIPMLGLIDGDPVNYDGQTDIVATTTKTFSQSVIVVGRAKSWVEKDFS
                     QDVTGGVPFMENVGNQVAKYWDNIDQKTLLAVLEGIFAMTGAKNLEFVNNHTFDITKE
                     TEAENQKVKAETLNSATQKACGDNKEKFALVIMHSVISTNLENLNLVARLKYTDPQGI
                     QREMKLGTWGGKLVLIDDNMPTREVAESSEGSNDGYTEYTTYVLGENSIDFEDVGTKV
                     TYEMDRVPGKNGGEDYLYSRQRKVFAPYGISFTKKAVATASPTDAELKNGANWELVND
                     SSNGSKTYINHKAIPIARIISRG"
     gene            28562..28894
                     /locus_tag="OUB02_000562"
     CDS             28562..28894
                     /locus_tag="OUB02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884360.1"
                     /translation="MNMYIDYEFYSTLGGEIPQDKFNKYAIRATKYIDYNTFNRIEEV
                     TEEIKIATCEIADLIYKSNLEGDKEIQSESIGSHSVTYSANNKTIEQKAYDILKMYLD
                     SDLLYRGV"
     gene            28896..29330
                     /locus_tag="OUB02_000563"
     CDS             28896..29330
                     /locus_tag="OUB02_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884361.1"
                     /translation="MFFKDNITLYNKYYDKYSDKTLYKRAYLIGVDYQGSKNIAVTDK
                     GLLSADSVKVIVPFSANARGKKYIDPFKYYELDEIEKEKFYTFKVGDIIVKEVVDFEI
                     TSVKPYTLRELQSKFDDVSIIKSVIKCDFGSIRMRHIELEAE"
     gene            29337..29684
                     /locus_tag="OUB02_000564"
     CDS             29337..29684
                     /locus_tag="OUB02_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HCT8884362.1"
                     /translation="MNVHVDIDVARIMRERGLERGGKAQKFFTHEVRRLSDSYVPKQS
                     GTLKNTAREEVDKIKYIQPYAKIHYYNNSGRGSEGMGAGGKRGKYWDKRMLADNKDEL
                     IGSVARFIGGSRE"
     gene            29681..30112
                     /locus_tag="OUB02_000565"
     CDS             29681..30112
                     /locus_tag="OUB02_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol resistance protein"
                     /protein_id="HCT8884363.1"
                     /translation="MTIVESVREFIKKCPYLDEFAKSINVEFLAEEFTSYTIETVPAE
                     TIVKKFVNGDSIKQFVFIFASRESYGSDVMQNIENSQFYEQFADWIYRENLNGNLPVL
                     DGNKESMSLEVSTTGYPVQTSIDTAQYQIQLKLKYFEKGEI"
     gene            30115..30915
                     /locus_tag="OUB02_000566"
     CDS             30115..30915
                     /locus_tag="OUB02_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884364.1"
                     /translation="MAGIETIQRYKIADYLGILGDDGQNSWELMGAGFNTLDENPSAQ
                     SDSKTYINDRNSTSAIKSYQTQFPFESDLIKSEKAIMELYKIGRDQATGADAERDYLR
                     VELFLPVEGKDNTFKARKFRVSVEVSGLAGAGGETIIVSGNLNSVGNFVDGEFNTQTK
                     IFTEVGEEAPPAESLKILTVNSIAGTNTGDTKVTVTPGLESGNSYKYKTGASVSVPAL
                     DADCSVGYTTWDGTEDITANEGDKILIVEVNSGNKAKKAGIATVTVKE"
     gene            30973..31335
                     /locus_tag="OUB02_000567"
     CDS             30973..31335
                     /locus_tag="OUB02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884365.1"
                     /translation="MLINNIELKDFDIYDADEYENIEKTIGLTITETGKVEKEATKGY
                     EMIRDICNLIFKCFDDIFGEGTSNKMFGDKRNLKVCSNAFFELIEEVNRQKQEGEEFF
                     ASAVSKYTPNRAQRRAKK"
     gene            31335..31943
                     /locus_tag="OUB02_000568"
     CDS             31335..31943
                     /locus_tag="OUB02_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HCT8884366.1"
                     /translation="MNMLIDVLPENVEIDGEEYKINTDFRISILFEMLIQDDSISDEE
                     KGKNALSLYYPVIPINTTVAIDKIIWFYSCGKKENDINDSCQSGAGGSSKGQIYSYDH
                     DDEYIYSAFLGQYGIDLQDIEKLHWWKFKALFKSLKEDTEIVKIMGYRAMDISGDMPK
                     AQKDFYRKMKKIHAIPLPKNEVEKINEIERALLNGGDISNLL"
     gene            32015..32656
                     /locus_tag="OUB02_000569"
     CDS             32015..32656
                     /locus_tag="OUB02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCT8884367.1"
                     /translation="MGFKFRKSINLGGGLKLNINKNSVSVSGGVKGARVSVNSKGKAT
                     TTLGVPGSGLYYQETTQLGGGKKKNGKSAGGSESSRQAQQLLKIINDCANIVNTTKDP
                     KTFFYRYNMLLDKSYALVAIEDNLNFSGQSPSQMLDSIIAKKTDTINDFLTRYYNEMS
                     SKLKELKTEKARMNNAYKFSSLAIDYKEHLNNDNKTKLNRLYKQLLNDVKKQD"
     gene            32762..38065
                     /locus_tag="OUB02_000570"
     CDS             32762..38065
                     /locus_tag="OUB02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCT8884368.1"
                     /translation="MADGSIIIDTLLNTEDAEKQLNNLADTMKKKAKSALAIAGIGAS
                     LGAIGKQAIDFGDEYQKAMNGFESATGNAEAKAKGFGEALQQVYANNFGEDMGDISEV
                     MSLISQKLDGIDASNIQEVTESAIMMRDTFDMDVGESLNGVNSMMRQFGISAKESYNL
                     IAQGAQQGLNQNDDLGDQLAEYSTYYAQMGFSAEEMFNMMKNGAESGVYQIDYLNDAL
                     KEFNIRAKDGSNGTKEAFEALGFNADELTKKFANGGSSAKEAFVEVTTALNNLDDNVL
                     KNQIGVQLFGTKFEDLEADAVTALTNIEGSISSSKDKLEEINKIKYNSFGEAITGIGR
                     QMQVNLLLPIAEGLLPVLNDLANKFAKAFQNENTKNAIRSTAESIGALIGTVVKLISL
                     ALTPLINVITFLIQHGSLVAPLITGIGVAFAGLKIAGIVRGIQKSFTDAQLAVRLFVT
                     GMAESGTTLTLYETIVGLVTKKISLAQVATNLWKGALTALGGPIGLTIVAVGALIAGF
                     VSLWKNNEGFRDAVINIWNNIKEVGEKIFGQIAKFFTETIPQAMSKFIDFAKENWQGL
                     ALLLVNPFIGGFKLIYDNCEGFRNTINKLFNSIKDALSKTMSFIKSNWQGLLLILVNP
                     FIGAFKLIYDNCEGFRNTVNSIFNKIVGIFKNVLSFIKNNWKSIGSMLLSPFVNGFKA
                     IYNSCVSFKNKVFSFFSSVVKGFVTFGKNIIAGIINGLSSGIGLVVDTVKNIGLTVVN
                     TFKKVLRIHSPSKVTTELGEFAGIGFANGIKNTSKDVVEATKELISAMEKNLETNKSK
                     FEKICEAITTALKNQYESQKDIQIKALDERLKVEEKASNDRLKVYEKEYNEKLKYLDT
                     ETNEKTNAIQEQIDAIDKQIEEEQKAEEEKAYNEKISDLNRKLATAKNQKEREKIQKE
                     IAEAQADRQKKLLDEQRQQEKNRLKEQIENIKTEASNKKEQYKEEYDNQKEAESKKLE
                     LIKESNASQKEEIEKYFSELLEETNIQNEARRLLLQKNSEEIIKLLSEYNPHWQDAGQ
                     SLADSLLNGVNSKKQSIQEAVKEAINLKEIIPTQEKELDRLKKKLEEYEKLKENASTS
                     SSSGGDLDTSAQDSLVNSGSSGLNTGELEKYADGVDDIKDSVEGLDLVSDELVTGTVP
                     KIEDSAGKLSESIKNGFSGIGDFFGGIEKWLRDTDKNINEWLASAKNSIGDFFSKVKD
                     KFKEGIESIGGFFCGLGNKISEGFARAGDFFAGLGDKAQQGFASIKDKAVEIFSGLGN
                     WFKETWSKIGDAIGNGLKSGLDFFTSTVPEWFAGIGDKIKEECGKIGEKISSFFNETM
                     PAIINSIAEWFKQIPYNIGFIVGSIARFFVDLGTNLYAWATETLPGIINSIIEWFVSL
                     PEKIGEFLSATLQNIQTWGENFKVSVSEFFSTIWENITQFFTELPEKISEWFNTVVET
                     VSSWGENLKQLATDIFNQFVDNVLIPVSQLPGKIWEKFADIINKVNEWGSNLLQKGLD
                     IAGKFLENVMKFFSELPKKIKEKLDDIIRKVTDWGTNLINKALEIAKNFFNNIYNTVS
                     QLPGKFKEWLDNIISNVISWGTNLVKQAGEAGKNMANAVKDALKDLPSRIMSIGKDVV
                     RGLWEGITGMGGWLKDKVFDFAGDVINGFKDGFGVHSPSIIMRDLIGRNLVRDVGVGI
                     DVETPGLKEKIEKNISELTGKLKATVNFETSKMQANIVASTDFKAEKETSMMSSNKTD
                     EANENQAGIKLNIENFVNNRQQDIENLFDEILFLAKRKGAL"
     gene            38188..38922
                     /locus_tag="OUB02_000571"
     CDS             38188..38922
                     /locus_tag="OUB02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCT8884369.1"
                     /translation="MFFVYDGRDSREFGLKIYNINDLSAPQMEVERVGVPGRDGDLLL
                     KKGFGNFTLTIECDIDARQSNIEEVATEIKKWLQGDISYKKLFLSNSDFYYLASCNNK
                     LDITRNFKNFASCLLTFDCYPFRYAEEKIISLNVLNLKSATITNFYRESKPVLYIEGA
                     GDISIKINTQSIVLRGVSENGILSDLIIDSERMNVYRINKENNIIVNENNKLFSDFPI
                     FEEGENRISWEGDIKSIKINPRWNIL"
     gene            39052..41052
                     /locus_tag="OUB02_000572"
     CDS             39052..41052
                     /locus_tag="OUB02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT8884370.1"
                     /translation="MREEENGVFELEFTIYEDSFLFKEIKTDRLVKADASPDFKNQLF
                     RIYYISKNLGGYINIKAQHIKYDLLNNFIESLNLNDVGCEEALEKVFRACEEENRFRG
                     HSDIKVRNTINIEMQSPYSAICEGENSLIQKFEPTAKLFFDNFDAYLNYQRGESKNVL
                     LAYRKNITGLEAEYDTQDIVTKIYPFATYEDEMITLTEKYIVSPNLNKYATQKIVAID
                     FSSDEVSVEEELREKCKDYFKYNQVDLPKILYKVNFVDLSTTVNYKDYKMLETVNLGD
                     EVIIRDFNLNINATARVVKTDYSPINKKYYSIEVGDLINHLDFLNNKFNNIENKIDTV
                     KKAIDNVKVDDSEFPDTLPNIPVLTAEGLFAFINLTWTFTNKSYYKYEVYASQIKDFV
                     PDTINFTNRIFTGQASAYVHQAEPFQTWYFRVRAVNSHGNVTEFSNQVEAQTTKMSDG
                     ATWIQEGAIADALIGQLKLDRGWFGQLKGSYINARELVVENDNGVKTMEVDSFGDVHF
                     NPNTLKVAFNNISPYVLIDDEGLKLISGKGFTKMTDSGLYTKFYTDSDVTESWYLQES
                     GYTYFDYKDSKIIIDLPKKFAGKNFKATCAIRSTSTNASCLSYFSTSATVKKSDTNPQ
                     LELSASVRGVSYEMSGNQTIGYWLGMNFFSEGFITVQYYVYA"
     gene            41077..41319
                     /locus_tag="OUB02_000573"
     CDS             41077..41319
                     /locus_tag="OUB02_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884371.1"
                     /translation="MVTIFYSKRTGEIYNCIKSEVEQDYTCFADREEDYRQILDKIVV
                     EDIPEILNPRDYKIENGKFVLKEKEIIKEFTPKMVE"
     gene            41334..>42172
                     /locus_tag="OUB02_000574"
     CDS             41334..>42172
                     /locus_tag="OUB02_000574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884372.1"
                     /translation="MRDRIYTVDINTKSYQVAKYKQYDNGIEFKINLLENNIEKDLTG
                     YTAIANFQRPDRKIVYQSCTIENSIVTTLIENNITAVAGDVTVEFTFYKDDLVVTTFS
                     LKINIEKSIDKNSITEEPKWDYISVTINQVKEVVEGIEEIKETEEARKEAEIERVKEF
                     NSIKETFDSKVTEVTDAKNTMISDVNTTKDTLTSEVTDAKTDLISVVTTAKENMINEV
                     SAVKTELETAEAERVEEFNNIKETFDSKVTEVTDAKNTMISDVNTTKGTLTSEVTDAK
                     TDL"
BASE COUNT        16743 a         4915 c         7929 g        12585 t
ORIGIN      
        1 tactttaata gacgaagtta acacagttaa aacagaagtt ataacagcta aaaataatat
       61 gattagtgaa gtaacaacag caaaagaaac tatgcagaca gaagttacag acgctattaa
      121 tgcaatcccc gcaaaagaag aattaaaagg ggttggaata gaaataaaag gaagtttaga
      181 taatatatca gcacttccaa ctaatccgac tttaagtgat gcttactttg taaaaagtgc
      241 tacaatagaa aatcaaattg atttgtatgt gtgggataat accagttgga ttaaagttcc
      301 tgatataaaa atcaaaggtg aaaatgggga taatttagag tttaattgga atagtacaca
      361 gctaggtgtt agggtagaag gtcaaacaga ttatagctat gttgacctaa aagggcaaaa
      421 aggcgataaa ggggacagca tagaatttaa ttggaatggg acacagctag gtgttaaaat
      481 agagggtcaa gaaaattata gttatacaga attaaaaggg gagcagggtt atactccaac
      541 aatcggagaa aacggaaact ggtggattaa taatatagat actaaaaaac ccgctcgtgg
      601 tgcaagttta aggattttag gaaaattaga ttctatagat aatttaccat tagaccctac
      661 tatcggagat tgctggatta tagggagaaa tatttatata tatcaaacta aatgggaaga
      721 cttaggttct ctagcgggcg tggacggcaa gaacttagaa tttaattggg atggcacgca
      781 gttaggcgtt aggcaacaat acgaattaga ttataagtat atagacctca aaggtgacaa
      841 tatcgaattt aattgggatg gcacacagct cggtgtcaga atagaaggac aggaaaatta
      901 tacttataca gatttaaaag gtcaaaaagg cgatagtatc gaatttaatt gggatggcac
      961 acagttaggt gttagaatag aaggacaaga ggactatagc tatacaaatt taaaaggagc
     1021 aacaggaaat aaattagaat ttaattggag cggaagtcag ctaggcatta gagaagaagg
     1081 acaaacggaa tatatatata cagagcttag aggggaacaa ggttatactc cagcaatcgg
     1141 cgaaaacggt aattggttta taaatggaga agacacgggg aaagcgtcaa aaggaaaagt
     1201 tacatggaat gagttattag aaaaaccgaa agagttagat tacattaaaa aaagcacgac
     1261 ttttaattca gacggctcta taacagatat tttagactcg gttagtaaaa ctataactaa
     1321 atttaatgca gatgggacta tagtagacga aaaatatata gataatgtat tagtaagtaa
     1381 agtaaaaacg acttttaaag gtaatcaaat agaagaaata aaagaagagg tgagttgatg
     1441 agttgggcgg agatatacaa agtaaatagt gacttgcaag gcgagccact taattttcta
     1501 agttatttgc aagatataaa actaaatgga ttagatagtt atgtgctgtt tattggaaat
     1561 gctaggatat gggaagaatt atatttaaat agtttatatt tattttctga tagaggaata
     1621 agagaaacag tttacacagc tttttcagaa actgatattg ataatttatt taataaaagc
     1681 actaagctag gggaacaatt aaatgccttt tatagaacag atatatttag cttaggaaat
     1741 gctgataatg tagtaaaagg aatgactatg gagcattata attcattaga ggaaaaattt
     1801 aaagcagggt atgatagata tgttacaaga gaacaagaaa agtcaactat aggagcatgg
     1861 tttaattcta cttttagctt agataatact gatttagaaa atcttacaac tatagaagaa
     1921 atattagcaa atgtggaagc tactaatgca atacttaata atagtaatgc aatagtagct
     1981 ttaactatgt gtaaatcaag catggacgca gttgtagcaa gttcaaatgc tatggattta
     2041 ttaggtcaat atattttaag aattacaaca gagtcaccag tcattagagc aattttaaaa
     2101 aataatgtga ttagggatgc gattataaat agtgatgaag ctatgacaca aatttcaagt
     2161 aacgaaaact ccgtaatgga aatatttaat gacttagaag ctacaaaagt attagtgcaa
     2221 aaccaaaata gcataaataa aatattaact aataatgtta ctgttgaaaa aataatccct
     2281 aatttattag aaatgaagta taatttacaa acttctttaa attatataaa tacaataaaa
     2341 tcaaatattg cttctggtaa aggtcaaatt atggcaataa cttataatga agaaatattc
     2401 ccgattttaa aaaatgcagc aaaaaattat gatggtatgg aaaacacgcg taatatatta
     2461 caacgagata ttgaggaaaa aataaaaatt tcggatgcaa ttttagaaag ttcaatcgca
     2521 atgacaactt ttgtaaataa ttctattata gtaaataaag ttggtgacag agcgggaatt
     2581 atagaaagta ttctcagtca aacagtgtca ttaaatgctt ttatgaaaag tacaacagct
     2641 ataaaagttt tagtaaataa agctactgca tttactaaga tagtaaataa ttcaacagct
     2701 ttcaatgcta tgcttactat ttcagggaat ataagttctg ttgcaaatag ttcaacagct
     2761 atgaatataa ttgcaaacaa cgcacaagct atggcaacag ttgcaagaaa cgatacagtt
     2821 ttgactgttt ttataaatgc ttcaacagct atgactgcaa ttgcgggaaa tgtgacatct
     2881 atgtcggcaa ttgtaggcta ttcgggagct atgaataaaa taacaacatc agcatttgcg
     2941 cttaatagaa tattcaagag tactataggc aaggatatat taattagtaa taatgcaatt
     3001 ttacaaactt ataaaaatgg tatacataac acattgaaaa gctcctctgg tcaatttagg
     3061 ttaattacat ccccaacgga ttcggattct gcaccaactc aaattataaa tagttcttat
     3121 gtaggagtaa cttattgtta tggatataga ggtggaactg gtaaatttgc tatcgtatat
     3181 catggtcaca aaacaagtgc agaagcaggc agaggggacg gtgacaaaga tgaaaccaag
     3241 aaatttataa cactaggtgg gactaaattc gtggaaagtg gagatggata ttttaattat
     3301 tcaatatatc aagcaattta atgtttataa gtcatctaaa agattagatt aatagtctaa
     3361 tcttttttta tataaaaaaa taaataatca aaattaggag gaaagttatg gaacaattat
     3421 taacagaatt aagtagttta ggagcaatag gcatattatg cgctttgcta tttaaaaaca
     3481 ctatgcagga gaaaaaagaa gatagagata tgtataaaaa gacagtagaa aatttcatag
     3541 aactatctac acagcaacaa gagataaata agaatatact tgttgaaatg ggagcaatga
     3601 aaacagacgt agaagaaatc aaagaagatg taacagacat aaaagacatg ttacagaaag
     3661 aaggtcttta aatgaaagta gcactaacag cagggcatac gctaacagga aaaggaacag
     3721 gagcaacagg gtatataaac gaaggaacag aaaataggat acttatggat ttggttgtta
     3781 aatggcttaa aaaaggtggc gctactgtat atagtggtaa agtagataag tctaataact
     3841 atttagcaga gcaatgccaa atagccaata agcaaaatgt agatgtagct gtacaaatac
     3901 attttaatgc tgaccatacg acattaaaca aaatgggtac agagacaatt tataaaacta
     3961 ataatggtaa ggtatatgcc gaaagagtca acgagaaact agcaacaata tttaaaaata
     4021 gaggtgcaaa atcggacgca agaggtcttt actggcttag tcatacaaaa gctcctgcaa
     4081 tattaataga agtgtgcttc gtagatagta aagcagatac agattattat attagacata
     4141 aagacatagt tgctaaatta atagcagaag gtattttaaa taagacaata gataataaag
     4201 agaatggtga gggaagaatc atgtacaaac atacaatcgt ttatgatgga gcagttgaca
     4261 aaatacctgc aactgtagtt ggctggggat ataatgatgg taaaatacta atatgtgata
     4321 taaaagatta cataccaggt cagacagaaa atttatatgt tgttggtggt ggagcatgtg
     4381 agaagatagg ttccatgact aaagaaaatt ataccatgat aaaaggtaat gatagatttg
     4441 atacacttta taaagcatta gattttatta agaaataaat ttgaaagaag tagtaactaa
     4501 tttattatat ttctatattt gtttaaattg tgtgttgacc agcgcgcaat aatatcaaaa
     4561 gtaaatgata gatttaaaag aggtaacaac taatttgcta gttgctacct cttttttatt
     4621 ttatttttat ataaatttaa ttattatctt taattttacc aattaagtcg tccattattt
     4681 caaaagctaa ttcgtcagaa atggatatat cataattgtt agagcaatat tcttttacat
     4741 aaacttcgta tgtaccaaat ttaacattac ttaaataaaa atctaattcc gtttcgtaat
     4801 cctcaatata gtcctcgtga catccttcgt tagtttctat atcatataat tcattaacaa
     4861 tagtttcttt tagttgttca gtaatatttt tcatagaaac atcccctttt cattttttta
     4921 aaattagtaa ataaatatct tacttgctgt attctctttc aagagaatct atattaaaaa
     4981 cccatgactt tccgaaaagt ctacagtcaa caccctcaac aaatttacca tttttaatgt
     5041 tctgcttcaa ggtactttca gccttattgt actttttaga tgcgtcgctt aaagaaataa
     5101 aattactaaa attatttagt tgctctatat atccattata aactcccaca acaaaattaa
     5161 ctataacctc gtaattatct aagttttcaa tgatttcggc aggcataggt atgtctaact
     5221 tcataaaagc ctttaaaatt atttcgaatg ctttgtttct atcttggtat ctgtaaattg
     5281 catcatttaa ttctaaaatt ttatttctac ttaatacatt ctcacttccg attttaaaac
     5341 ctaaattata tagctcttta tttaattttg cattcataaa attcactcct tgttttgttt
     5401 atataattat tatagtcgat acaaacaaaa aaagcaataa aaatagtctt aaaaaatatt
     5461 tatagtcttt ttagactatt ttttattgac aaaatagtct tttaagacta taatataatt
     5521 aaagataaac aaaaggagga tttaaaaaat gaaaaagata actaaaaaag aaataataga
     5581 atttgtaaga gatgtagtag gagaatatca agattggaaa ttaaaatcat gtggcttcta
     5641 tataaaagat aatgaattaa attcttttgt aagctttgaa ggaaaaggaa ttgacataaa
     5701 tgtatataag gaaaactacg acgaaataat ttacatagaa gattatataa aagactacaa
     5761 gcgtaaggaa tataacttaa aagaaataga ttctatcata tacgaagatg taaacgaaat
     5821 gattagtaac tataatgaaa aataaaaaaa tgaaaataaa ataaaaatta ctggaggaaa
     5881 ttaaaatgaa taaacaaaaa gctagaagat ttttaagagt tatagatatg aatatagata
     5941 aaatagagga agaagctata aaagctttta aagaaagttg tttaatcaaa gagactaata
     6001 atataaaaat ttatatcgat atacaaggaa aagttgaagc gatagcagtt caaacttggg
     6061 ctaaactttt agatgataac aaagaaatta atattttcac attaaatcaa acaccaactc
     6121 atttaaacga tttgtttgga gaaatttgtt acgtaaacga ttatgaagaa tttgaaaatt
     6181 ggtgtgaaaa tgagtgggaa aatttggatt gggatagtta taaaaaattc aataaagaaa
     6241 atttcgaaga aattgctgaa agaaatatag acgatagcac atcagttttt ttagaagaat
     6301 tacaaaaaag cattgaaagt tgtaaacaag aattgcaaaa tgtaattgaa aattaagtag
     6361 aagagtatta ttattaatgt atatagtatc gcttttaaga ttaactacga gggatttaaa
     6421 atatttttta atttatttag aaataatatt gtaataagaa ttttaagatt aactatgagg
     6481 gatttaaatg tcaatttaat ttatatagaa ataatataat atccaatttt agattaacta
     6541 tgcggaaata aaaacagtgt attcaataaa tacactgttt tttaatgttt agttattatg
     6601 ataacttaat ccttcgatta taaatcctac attcgttgtc atctctaatt cgttacttaa
     6661 cattattcct tgtgtaaatt cattgtcagc tggcaatgtt actctttttc tagcaattgc
     6721 ttctataatt gtttgaaata gtttaatttt catatcatta tcatgtccag cgaatcgtag
     6781 ataattcgtt acttcttgcg aaaacttatt caactccgat ttagtcattt tttcagataa
     6841 ctcttttcca atttgcagat aaacgttcat cttttccaac ccctttttag attatagtta
     6901 aaacatatta tttaaacaat attaatatat tgtaataata atattaaaat attacttaaa
     6961 tattatatat acaatattta agtaatatct aaacaatata aatataatat tatttacttt
     7021 ttcttagtct tatttcttct atttctctca ttgtatatat ttaaattctc ttcctttata
     7081 gtgacatcag ataaaatttg agttagtata tcgcttaata atttgcttac gtcgctgttc
     7141 atatcaatag aaaaagattt taatttgttt acaagttctt cattaagcgt tgcactcata
     7201 gttacctttt tacttttctt attaactaga ttatatatgt ttaatgtctt attgtctgta
     7261 gttgtagaac cattttgtac aattgttcca gtttttttaa aaactggttt ttctttatta
     7321 gataaaaggt cttcgttgaa acttggcatc tataacactc ctttcgcttt tagttcttct
     7381 aacatattag taaactcttg tctgctgaaa tggtctttag aatcttttaa aaagtctgtc
     7441 aaagatagtt ttttcattag agccttttct atgaactcgt tttttcttat gtatgtcttt
     7501 aacatcatgc tccttaattc actaaaacct tccaaataag aatcgaatac ttcacttacc
     7561 tgtgtttttt tcttttcaaa accaaccaat acgaaatttt ttatattgtc ctctttatct
     7621 agtaaagccc tgtcagcatc ccaaagttgt ttaaataatt ccgctccacg aagggacgac
     7681 acatttttat cttgtataag aacaattatg ctatcagcta aaaaaagtac gttcttagcg
     7741 attaggtcat aatgaggaga taaatcacaa atgatataat catatttact aaaagtatca
     7801 aaattttcca tgtaccactt ggctaaaatt ttttctttat catctttaaa attaaataaa
     7861 ttctcttcta gtaaagtcat ctgaatatca gaagctatta aatcaacatt agaatatttt
     7921 ttattaaaag acttaatgat aatatcatca gcactgcaat ttttcaaaat atcaagagta
     7981 gttttatcgt tatgatttac tttataaaca tattgtgtta gatttgcttg tacatctaag
     8041 tcccataaga gtattttttt atctttatct tttgcaagtt catatgctgt cattatagac
     8101 gttgttgtct tatatattcc tccttttatg ttaaagtacg taagtagctt tgtattagcc
     8161 attttctcca cctcattatt atatttacaa tattataata atatcatagt attgttttta
     8221 taatattgca acaatatatt tatattatta attaaaatat ttaaacaata taaatacaat
     8281 atttaaatat tgcattcata atattaatat attatattta caatattaaa atattgtata
     8341 tgcaatacta aaataatatt tttataatat tacagtatta tttttatatt atagtaaaat
     8401 ttgaaaaaga aggctaagaa aaagcaacct tctttttata gaaaaagtca tttattctta
     8461 gccttttcaa ttattatcct gtcgccctcg aatgttgccg taacattagg gttttctctt
     8521 gttatgccca tttcatcagc ccatttttta ggaacactca ttctaggagt aaacgaccca
     8581 ctcccacctt tgtgaaagtt aaactttaaa tctctttttt ctttttccat tcattagctc
     8641 cttttttgct gtattacaaa gaaaaataat aatacaacac taaaaactaa accgagcatg
     8701 ttgcttgtac tagagtttaa tactatagat actatattta agactatagt tattaaaact
     8761 aaaacaatgc aaatcttata aaatttatca ctcatgtttt ttagaaatgt gttataatat
     8821 tagaaagaag tcacctagag agaagggcta ttccctctag gcttatcctt acagttcttt
     8881 taaaattctt agaactgcga ggattatgtt aagtaataat aggatgaagg agaaaacttt
     8941 tttaactatg ttaaatttgt tttctctttt ctttttactt ctttctaatc tccccattcc
     9001 cgttcctcct ttcttttatt tttttgcttt ctcacctcct tctctataaa tatattatac
     9061 catgacgggt cgtcaaaaac aatagttttc tcctattttt taaacaaatt ttggtaaaaa
     9121 gtaaagattt tagtatatta gaaatgatat aaattcgtat cttttaaaga aaatgtgata
     9181 taataaaagc aagagaactg tatttcagtc ataagagtag agttttagtc taaaataaaa
     9241 aattatttaa actttttgaa tttgattttt aaatcaaatt cccaaccact cttattggcg
     9301 tttgagtggt tttttatttt atcatatatg aaagctgata taacaccagc tagtatactc
     9361 aataaaaaat ttatcaccat attgttccac ctccttccgt tatggaattt ggcgtttaaa
     9421 tatggaaata gccactctta cgcttttcaa tcactttttc cttgctaaaa ttattataac
     9481 atatatttac ataaaaaaag acttagttta tatactaagt cttttaaaat gcagtatttt
     9541 agaaaatata tttttaaaat ataatttatt ttcaaaaaga gtatattttt ttataattta
     9601 aatgtgctat aattataatc acaacgaaca gaaaactttt ttttaattgg tttaaaaaaa
     9661 tctacaaaaa aataaacaag gaacatgtac ttgccgtcaa acaaaccatg ttccttacac
     9721 cggaggttag acctctctta aggataatat atccttgttt ttctatactt atatttatat
     9781 tataagacaa agtgtagaaa aacacaaggt ttttagggta attttaatag taaaattatt
     9841 acaaaaaatc aaaaaaagag cgtcagcctc caggtaaaaa atggagggat aaagatggaa
     9901 aataataata aaaataatat agataagatg tttttagaag aatgtagcgc tgaaagagca
     9961 gagcatgaaa aagaaaatta ttcaaatttc aatagacatg aaacatatga aataaaaaaa
    10021 tatataaacg aaaaaaaact aggaattaaa acggctatag ccaacgcgat aaatggcggg
    10081 aaaaccctgt ttattgcacc gacaggagca ggaaaaagtt atagctttat aaatacatta
    10141 aaaaaattaa aaacaaaagc gttattcata cttccaaatg cctcaaatgt agagcaggca
    10201 atgcacgagt atgatatacc aggagcatat gacaaaatac ctgttagcga agcatttaaa
    10261 agtggcaact tagttgtaat gacttgggat aaaaccgaaa aacttataaa tacagactta
    10321 agtgaataca taattgtagt tgatgagata catcaaactt atacagatgc atatcgagga
    10381 aaagcaataa aaaatttaaa taatataatg tctaagtgca aaggaagaat agacattaca
    10441 gcaacaccaa caaaacttga atttgaaata tatgactaca taatagagta cactcaaaag
    10501 caaaaaacag aatataaagt taaattgtac aacgactttg acaataaaaa ttttactgaa
    10561 atgattaaca taataaataa atctaaaaac agcgccatgt tgatgaatga tatatcaaca
    10621 ttagaattta taagagattc agtgaacaaa aaagttggag ttgtatatgc agatggaaaa
    10681 gaagaaaatg aattgtacaa tagaatagtt aaaaattcag acatgaaaga ctatgaaacg
    10741 cttttaaata caacaacaat cctagctggt gtaaatataa acaacaaaga tatcacagat
    10801 ataattattg ttaatataaa agatgttgga gctataaaac agtatgttgc aaggtttaga
    10861 aacttaaaaa aagtaaaggt acacatattc aataaatatg aagaagagtg caatgtatac
    10921 aaaattgaat ggctagttaa caaaaatata gaaaaagcaa ctatcttaaa agatgcttat
    10981 aacaaagtat ctaaacacat gttacagttt gaaacagttg gaataaatgc aacgccaatt
    11041 cgcatagact caaatgtgta ctactgtaca aaagataatt gctataaagt agataaatta
    11101 tatataaaaa gtcaagttta cagcaactat tacaatacaa gaacaattca aagttttaaa
    11161 gtgctattag aagaatattt tgaaaatgtt aatataactg atgctaaaga agttggcaca
    11221 aatgaaaaag agttaaaaga gtacaagaaa gttgtaaaag aaattaaaga tgcaacaaga
    11281 gaaattttaa aagaacataa agaaattcta gtaggttata gacagattaa aagcgattct
    11341 aagagctttt cactaatgca gtatcataat gatatgaaac taagttcaaa agggtgctta
    11401 gaggcttaca gagcgtatga catacataat ttggtaaaga aaagtaaaag taatagcatg
    11461 ttagaactat attctaatta tgtactagat aataagttcg acttagacct tgcgtggaaa
    11521 ttagcaaatt tatcaaatcg aaagcgtgga gcagtattta ataaaataaa tactttgata
    11581 tatagagaat taaaagaaga atacccagaa tttttgaatg atgaacttat tcaagttaca
    11641 gtatttaatt tcatagataa gctatttggg attggaacat cttatacaga acttcattta
    11701 caagagttaa gtaacgactt aagaacgttt ttaggagaga attgggggtt aaacactaaa
    11761 aaaataggtt gtatattgaa cgaaattttc gtaatagaaa agaaaaaata tagaagtgtg
    11821 aaccacttag aaaatatctt tttttataaa aatatgaatc ctaagtggtt cacagtagaa
    11881 gttaaacaaa tacaacttag caatataaaa agatatgtaa ctgttgatga cataaaaaaa
    11941 gatttagaat tagacgaaaa agataagagt ttagaacgtg ctataaaaca tacgaaagac
    12001 aggatgttaa actctttaga tgaagatgaa aaactattgc tattaaaagg attgatgtaa
    12061 aaatacaaag aatgtgtcca cacttagaaa atatcttttt ttataaaaat attaatccta
    12121 agtgtggaca ggaaagaata aatttgtatc aaattgaaag atacatcaac attgaagata
    12181 taaaaaatga tttggaatta gacgaagaag ataagagttt agaaaatgct ataaaacata
    12241 caaaagatag aatgttaaac tctttagatg aagatgaaaa actgttgcta ctaaaaggtt
    12301 ttatgtaaaa acatatggag ggatgaatat gaaattaaga attgggatta tagataaata
    12361 tgtggctgag gcaagttatg agttatcaga acttttaatt gatgctttta ataaatatga
    12421 tgaatttgtt gaggctataa gaggtggaga aattcgcata gtcccattta atttgggatt
    12481 tcaagctttg ctggaagagg attctactta tgatgagtat gaattttatt acgatgatga
    12541 ggatgataaa tgtttttttc atattaaata tagaaatgtt aaagatgttg aactttcaaa
    12601 agaacttttg ctagaactta taaaagttaa tttgcccgca ggagcaaaat taattgtgaa
    12661 caactagaaa tttgtgttta gatacaaaaa gccgggagta ggaatatata tataaattcc
    12721 tactcccggc tttttgatat gagagctgta gggtttaaat atttgaccct atagttttta
    12781 aatgtgagaa aattcaaagt aaattgaatt gggattattt tccactagca aaatttgcta
    12841 aaaggtacta ttattgtagt ataaatgttt aattacgaca aaaaatcgta catgcgttct
    12901 tgtaatatcg aacaaaaagg ggtataatta aaattgtaag ttatttttta caaattttaa
    12961 gaatgtattg ttctattggt gtataggggg atgatttgat tgaaagagga aataaaagag
    13021 atggtagacg aaattaggga tagagtcaaa tctttaggaa aacaatttca agtgctatta
    13081 atcaatatca gtaaatacca gggggtaatc atgaaaaaat cacaaattaa aacaaaaaac
    13141 agatacattg agaataagat agaaacagct atttgtgtaa atgaattgaa atatatttct
    13201 gttgatgatt atgttttctt aaaagaatgt atagaaaaaa tttatataaa aaataaaagg
    13261 acttgatttt ttatcaagtc cttttatttt ttaattgaaa ctaattattt ttcttgtgta
    13321 aaagctctat catttgtaaa atagcatttc tatcttcgtg ttctagctca taaagcttat
    13381 tagttatctc ttttacatct tcatcaattt ctagttcaga taaacaatcc ttataaatat
    13441 caccaatacc ttttcttagc cattcttcgt ttacatcaaa ttccctgcaa atatcattaa
    13501 tacttctttc tgttaatact cttttccctt tttccagtaa agaaatttgg tcttgcgata
    13561 agaaaatttt ttctccaaaa gccttttgtg agagtttttc gtttgttctt aataaattta
    13621 accttttgcc aatttctttg tttattgttt ccttagaaat ttcatccatt ttttaatcct
    13681 ccttttgtaa ttattgtatc ataaaaatac gactttgtca ttgattaaaa aatatttacg
    13741 acaaaatcat aaaaatataa aaaaaggtat tgcattttaa cgacaaagta gttataatta
    13801 aattaagctt aacgacaaag caaaaaggag gtataaaatg gtttgttcta gcaaagaaaa
    13861 aatagaaaca gctctaatga tagaattttt aaaaaaaaac gaccctagaa attgggaatc
    13921 tatagaaact ctaatatcaa gtacttacac attaagtaac tttaaaaaaa gaaataaaat
    13981 atctcaaaaa agaggtgata aaattgaact ttaataaata tcgaaaatgt gaaattgttc
    14041 aattatacga aacaaaattc aactgcaaca gaaattgcat tgttacatat gcagatgaaa
    14101 aagaattagt attgatgtac tacgacaaag aaatagaaga attaatatat aaaactttaa
    14161 ctaaagaaga tttgatattt aatgactatg aacttaagtt gctatcttaa taaataattt
    14221 ataagtggaa gggggtgagg gattggaact tttaaaagat tcaaggaaaa gagattggtt
    14281 ttgggtagaa aacgatttaa ttgatagaga agatttgact atttatgaaa aaatgattta
    14341 cataacatta gttagacatt taaacgaaaa tagtttttgt tttccgagtt acaaaactat
    14401 ttctaaaaaa agtggatgta gtgaaagtca agtcaagaaa gttataaaaa gcttagaaaa
    14461 taaagaactt ataaaaaaag aaaatagaaa caaattaaat tctaaagaaa aagatagcaa
    14521 tatttatttt gtattgactg caaaaataag tagggcatgt agtgacctac ccgtaggtca
    14581 cagtgaaacc caccctaggg catgtggtga cctacccgtg gggcatgtgg tgtctagtaa
    14641 caagactaat attaacaaga ctaatttaac taataatata tatagttctg cagagcaaaa
    14701 agaagaaaag gcaaaagaca aagagatata taaaagaatc gttgattact taaataaaac
    14761 agcagataaa tcttttaaga gtacaacaaa gaaaactata tctttgattg atgcaagact
    14821 caaagaaggc tttacagaag aagaatttta caaggtcata gataacaaag tgttaaattg
    14881 gctagataat gataaaatga acgcttattt acgccctgag acgcttttcg gaaacaaatt
    14941 cgagtcttac ttaaacgaga cacctaaaaa ggccctaaaa ggcaatgaga gcgtcaaagg
    15001 aaaaacaaag acaaataata ataaatttgc taatttcaat caaacatttg ataaatattc
    15061 agaagatgaa cttgatgcaa ttataaagaa aagtcaaagt gctaaattta aataaaatta
    15121 aaaaaggtgg ggtaaaaaaa tggaaattaa tagcgtaaga gagttaagag aattgtttga
    15181 actttttaca gttataaaag cggaaggcaa caatgttgtt tcttgcaatg aaactgtaaa
    15241 cgagttaagt aacaaagaac taataaaagc aaatgaaaac aaatttaaaa aaccttggaa
    15301 tgatgctcta ttatatgaaa taaatgcttt agatataata aaaaagcata agcacagtga
    15361 tagaaaagaa aaggcatata aaacttatct aggattagaa aatgcagata tagaagaaat
    15421 ttataaaatt cattatggag ataaagaaaa ttctgaaact gttgcaagtt gcgaagaaat
    15481 tagttcagca aaaacggata caatatcttg caatcatgac agtgtttcaa aaggtaaaga
    15541 tactagtgat tcagaatgta cttgttttat ttgttctaaa aaatagaaaa tcaagaagat
    15601 gtatttaaaa tgcctttttt aacacgagat tatttgagtt cagcaaaaga aataaaaaat
    15661 attgaaattt gtgagaaatg tttggatggt aattcgtgga gtattataag aagaaagcag
    15721 tttaagaaaa tgaagaaata aatatttaaa ttagtatctt tacatgtgaa aaaggagagt
    15781 gaaaaaatga taaaaaataa tgttttaagt tatgaagaaa aaataaaagt attaaaaaaa
    15841 catggttata gtgattttac caaagaatat gttttaaaag aatttgacaa ggaacatgca
    15901 ttttacaaat catttttaag agcaatagaa aaagaagatg atggaaaagg tgtgttaatt
    15961 aaaaaaattg gaaaaggtca gcatcaattt tcaaaaggtt ttataaaaac acacactatc
    16021 agtgagttat tggaagaagc aaaacgtatt agaaaaatat tagaataagg cttagaaatg
    16081 tctataatct aaaccttata cttaattaaa atttacctaa tatgtactct attgcgatag
    16141 gtagggacat atttaaaaaa cttgaagtgg aactttttat tttattccat ttagtgttat
    16201 ctcgtatttt atctaaatat tgataacctt ttaaagacaa ctgcatgacc tctaaaacag
    16261 tatcatcacc gtaattaaca tcgtcaacga gattattttg ataaattaaa tttatgtgat
    16321 gaacaatagt tgacttatcg tatgtgagat tatctagtat agtatcaaat tgtaatacag
    16381 gcgaaatggg agttgtatta gattcaataa gtaataaaat atctcttata caatcttggt
    16441 tagtaaccat agtatcacct ccttttgctt tagtatagca caggagaaac gagacaataa
    16501 taaattttac tgtaacagaa cgagaaggag caaagcaaat gcaaaagaaa attttagaga
    16561 tgagtatatt ggaaaaagct gatttaatta gtaagaaaca aaagaaacta caagaaaaat
    16621 ataatctaag taagggagaa ttgttttcta ttgcttgtaa tgaagttaaa aaaatagaag
    16681 agcaggaggg aaaatatttt gaatataggt agttatacat tagttaagaa acaactaatt
    16741 gaagaattga aagaagaaat taagcaaaaa gaagaaaact taaaagaacg tgagaaatac
    16801 ataaaagaat taaaagagag cttagaatat tttgcgaggg atgcgttaga atcagaagaa
    16861 tgcataatag atattgaaaa tattttgata gaacaagctg tagaattaga agcttataat
    16921 ttattcatag attgcatttt aaacaaagat ataagaaaag ctattgcgat ttataacaga
    16981 actaaaagca tcagaattaa gcataaaaat attaattcta tatctaagaa aattgaaaaa
    17041 ataaaacaaa taatttcctt gtagaaatag gagaggttta ataatggata agttcgtaac
    17101 atgcgctaga tgcgaaagaa caataaatgt agaagaaaat aattatgtga agtatgaaga
    17161 agaaacttta aatttaagtt ttacgttgta tttctgttta ggctgcgtag ataaattaat
    17221 tgaagaacaa ttaaaatcaa tggagggaga aaatgaataa gattagtgtt acacaagcat
    17281 tagaaaaatt tgatagttta ctagataatt ggaacgacct tccgaatcat gtttacaaaa
    17341 aagaatatag aggtaaattt tatgactgga taaagtcact tgaaagaaaa gatagtttac
    17401 aaaattataa gatagttgaa gttttaaata atgaacgaaa tggagaagaa gcgccgtttt
    17461 ggaattgaag aaatataact tgaaaaggac ttaaactttt gttagataga agggatgtat
    17521 ataaatatga aaatgataaa tgtttttaaa ttatcatcaa tgtattgtat ttgccccaaa
    17581 tgcgggagcg ataaacttgg aaaaaatgaa ggaaagttaa ttgttgatga acatacttat
    17641 tacagggaat gtaaatgcgg ttttaatgtt cttgtagatg aaagagaaga cgaaatataa
    17701 ggaaggtgct ggaaatgaat agtgttattt taattggaag attaacaaaa gacccagaac
    17761 ttaaatatat accgggttct ggaacagttg tatcgacttt tacaatagca gtagatagag
    17821 attatattaa aaaagatgga actaaagaaa ctgattttat acctattgag gtgatgggga
    17881 agttagcaga agtatgtgct aataatttag gtaaaggtag gttagtagcg gttgaagggt
    17941 ctataagggt taatctctat gaaaaagatg gcgagaaaag aacttataca aaagttcatg
    18001 ccaataaaat taaattttta gattataaaa aggatgatgc tggaaaggag tatatgtttg
    18061 agccgggagg cttagaccca caaggcttcc aggctataga cgacggtata cctttttaag
    18121 aaggaagtgg ttgaacattg aatgaaaagt ggaaacaata tatagaaaat gttttagaat
    18181 cgagaggtta ttgcataaat agaatgccat atgtatattc atatgatttt cttgtaaata
    18241 gcaatattaa gataaatgta gaaatatcta atctttataa aaatgaggaa aatgaatatc
    18301 actattttga attagaaaaa gaatatcata gttgcgatat ttatatcctt attgcacttg
    18361 atgaagtcgg aagtgctttg aaaatattag taataccagc tagcaattta atgggacaaa
    18421 agcaaataat tattaataac aaaagtaagt atgataaatt tgattcaagg tttgaactta
    18481 taaaggtata tgataatttt tacaatgacc ttgattatgt tatatagagg tgttttaggt
    18541 gtttaaaaaa gtagaaaaaa attttagtgg ttcagttaat ccaaatttta tggaagatga
    18601 cccaactttt aaaaattatt tagcgttata ttgttgcata aaaccagtta aaatccgtgg
    18661 aaataaaaaa gttttggcta gttccactaa ggcgtgtagc gcattgaaaa ttagagaacg
    18721 tcagaaaaaa gaacgtaaaa gggtaagact tgtaaatgca aagacaggtg aaataaaaga
    18781 aatggctata gatgaagcag aaaatttttt gaatgttaaa gacttgtatc cagttattcg
    18841 gcgagggaga ccaactcgaa ctggttggta tgtaaaggat gttgaagatg aaggagacta
    18901 aatataacgt tatatatttc aattcagaaa ctttaaaaat ggataagaga agttttgacg
    18961 ctttaaaaga tgctagagca tttaaaaagg aaaaagagaa aaaatacaaa aatgttgaga
    19021 ttattaaaaa gacaattata gaaaaattga taatgtagga agtgaattta tggcaaaaaa
    19081 gaaaaggatt tgttcatggt gtggtaagtc gttttttaca gagtcgaaaa atagattttg
    19141 ctgtaaatca tgcgaaagaa agtataaaaa agacaaagaa aaaaacgcaa gttcttgctt
    19201 agttgaagaa accaaagaat atttaattga actaggaaat aagaaaagaa gagtagatat
    19261 tctaagaaaa gagatagatt ttttaaaaga taaagagttt tatagagaga taaatttcaa
    19321 tgaactaggg tttaaaatac attctagccc gaagggcata gatgaaatga ttatgaataa
    19381 tgaggataaa atatcattta aggaaagtga aattgatttt atagaatata gattgagact
    19441 aatggatatt tacataagtg aacttacaga agagaagcaa caaattataa aatttatgta
    19501 tttttatgat ggcgcaagta aactttctaa tgaagaaatt gcctcaaaga ttaaatgtag
    19561 tcgcagcaat gtttattata aacacaagga agcattgaaa gaacttgccg aaatgctttt
    19621 tgaaagtgga agtcttttat agaacaaaaa aagacggttt ttaagacaaa gttagaacac
    19681 tcttttacgt ttatatagtg tataatatta atatcaagaa gtttaaaatt aaatagatat
    19741 agatttgaag cctggcagga atgttgggct ttttataatg caaattaata ttttaagggg
    19801 gttgagaagg tgaatagaag tgggtagtaa gaaaccagct aacccagtca aagacaaacg
    19861 tatggtctta aatatacaag aatacttaaa agaaaagagt gttagaaatt atgttttatt
    19921 tgtcttaggg gtggctacgg gctacagggc aggggattta gttaaactac aggtaaggga
    19981 cgttagaaat gcaatagacg aaggttattt cttaataatg gagagtaaaa aagaaaagac
    20041 taagaacata agaaagaaaa ataaaaagcc tagaaaagct ccaatagttc ctaatttaga
    20101 aagagtactc aaaagctaca tacgagataa aaaggactat gaatatatgt tcccatccag
    20161 gcaaaaatct gttactccgc atataggcgt tgagagagta acagtgatac tgaaagaagc
    20221 aggtagatac tttggactta agcatataac agcgcatagc atgcgtaaaa catacgctta
    20281 caccatatac gaagaaagtg gatttgatat tataagagtt aaggaaatgc ttggacattc
    20341 tagtatagaa gaaactaagg tatatctagg tcttaatgaa gaacaatatc aagaatatag
    20401 catgttttta aatgacctaa tagggtgatt tttttatttt gtctttttga atgtgggaaa
    20461 aataaaggcc tatatattca atgtattttt ttcgctatat ataaagtagg aaaagtaaaa
    20521 agtgaatgtc tgattctcta agaaaacccc atattcgtta aaataggcgt tagccattat
    20581 aaatactggg atagaagcta tttaacgtta aaatgggtat ggtgaagctt aaggacatac
    20641 ggacaaaact taaaggacat ggaaaaatag agaatgaggt tgatgtaata aaaactataa
    20701 aattcacagg caaacaattc aattacgccc ttttacaaga gtaatgacaa tatttataag
    20761 tacttttgat tcatttaaaa aaattcactc gtattcataa ctgtacggta ttgactacga
    20821 tttgattatg tagtaaaatg aacatataga aggtagtcat aactaatggt caaaagtaag
    20881 gggtgtattt ttatgatatt tgggtattgt cgggtaagta gtaaagtaca aattgataat
    20941 aatagtttag aacagcagga gcaggaaata aaaagacatt atagtgatgc taaaatattt
    21001 agggaacaac atacagggtc aaccacgcac agacctattt ttgaggaaat ggttaagcaa
    21061 atgcgtgatg gtgataaatt agttgtaact aaattggata gacttgcaag aaatgcaaca
    21121 gaaggtatta atcttataca aaatttattt gatagtggta tttctgttca tgtgcttaat
    21181 gttgggcttt tagaaaatac tactcttggc aagtttttta taacaacctt gcttgcagtt
    21241 gcagaaatgg aaaggaatct aattttagaa cgaacttttg caggtaagga gatagcaaag
    21301 caaaatccta atttccggga aggtagacct aaaaaatata ctaagatgca aatggaacat
    21361 gctttagagt tattaaaaac taattcttat acgcaagtag agagaatgac agggattagt
    21421 aagagtacat tacaaagagc aaagcgaaaa caagaagact aggtacttag tcttttttta
    21481 ttgaaaggag ttgatttgta tttcaataaa aaattccaat cgagaaaaag cgtttgagat
    21541 ttacaaggag aaaaatggga gaataaaatt aattgatatc tctaaaatgt tagaagaaaa
    21601 aagttgcaat attagtcgtt ggaaaaaaat tgatagatgg gattatagac ttggaataaa
    21661 taagaaagtt ggcgcaccag ccggcaacca aaatgcttta gggcatgaag gaggcgcacc
    21721 agcgaataat caaaatgcta gaactcatgg atttttctct aagcttttgc ctgctagaac
    21781 ttacgagatt gttaaatata tcgagaagga tggtggcaat tctttagaca ttctttggaa
    21841 cagtatagta gttcagtatg cgaaaatatt agattctttt aagattaccc atgtcaaaaa
    21901 taaaaaagac catacaactg acattaagaa aaatggtaat aaaattaaag agtatgaaat
    21961 tcaacactct tgggataaag ttacaaatgc tatgaaggca gaggcgacag catttaaagc
    22021 tttgtccaaa atgataaaag actatgagga actacttcat aaaaattggg atttagcaac
    22081 agaagaacaa aaaagcagaa tagaaaacat aaaagcgaga acaaataagt tgactggtaa
    22141 tgacttagaa atagaggata tagaagagat agaggcagaa atctatggca gtaactaaga
    22201 agaaaactat accttttcga tttggagata agcacaaaga atatattaaa aagtgcgcta
    22261 tcaatacata caatatagcc gaaggcgctg taagagcggg taagacggtt gacaatgtat
    22321 ttgcctttgc gcatgagata aagacatcta aagataagat acatctagca acagggtcta
    22381 caagtgcaaa tgcaaaattg aacattggag atgctaatgg ttttggactt gaatatattt
    22441 tcagagggca atgtcattgg ggtaaattta agggcaatga atgtttgtat gtaaaaggta
    22501 tttcaactaa gcataagcaa aagattgtta tttttgccgg aggggctaag gctgatagtt
    22561 ataagaaaat aaggggtaac tcatatggca tgtggatagc aacagagata aatctgcacc
    22621 atgacaatac tatcaaagag gcttttaata gaattatagc atcaactaat cgtaaaattt
    22681 tttgggattt aaaccccgat aatcccaact cgtcaattta caaagaatat attgatgctt
    22741 atatgaaaaa agatagggaa ggcactttac taggtgggta taactatcag cactttacca
    22801 tccacgacaa tataacagtc tcggacgaga gaaaacgggc aattatatct caatatgacg
    22861 agggcagtat ttggtacaag agagatatac taggtcagcg ctgtgtagcc gaggggctta
    22921 tatatgaata ctttgctaat aataaagagc aatttaagac tgaaacagtt gacccactta
    22981 tggacgttat tattggagtg gattttgggg gtaacaaatc atatcatgcc tttgtcgcaa
    23041 ctggaattac ttataactat aaaaaagtaa tagctcttgc aagtgaaaga cctagtgcag
    23101 ataccagtcc agacgagttg aatgctttac ttataaactt tataaaaagg gtaataaatc
    23161 tgtatggtaa agttgattat gtttattgcg atagtgctga acaggtatta atacgaggca
    23221 ttaaaaatgc agtagaaaaa gaaaatttaa atgttagtgt tagaaatgct ttgaagagtg
    23281 ctattaacga tagaataaga cttacagact tattaattag tcaaggtaag tttgagtata
    23341 caaggtatag tgagactctt gttagcgcat tatgtggtgc tgtttgggat agcaaagaaa
    23401 ttaatgagga tgtaaggctt gatgatgggt cgagcgatat agacagtttg gatgcttttg
    23461 aatatacaat agagagatat ttaaaaagat ttataagggg tgactaaatg tttgatggat
    23521 taaaaaatgc tataaggggg gtgattaaca agttgtttaa taagagttct atacaaaatg
    23581 aattaaaggt agatatagct gttagtgata agatgtcaag agctattgat ttatggatgt
    23641 ctatgtataa gaataaatca ccttggataa atgaaataac taaaagttta aatttatctg
    23701 ctacaatttc aagtgaagtt gcaagacttg ttacccttga acttgaaagt gaagtagtcg
    23761 ggaatgactt tttaaatgaa caatatcaag atgtattgaa agatataaga aagtattgtg
    23821 agtatgcttg tggggttggt gggctggtat ttaaaccata tgtaagcgga gacaaaatag
    23881 aagttgatta tgtgcaggca agtaattttt ttcctgttga atatagttct acaggtgata
    23941 ttatatcagc tattttttca gaaataaaag ttaaaggtga tattaaatac actagattag
    24001 aatatcataa ttggacttct acttcttata ctatctctaa ttacgcatat aagagcaata
    24061 ccgcaaaatt aattggctat tcagatgatt tagggaagag gattaaatta agtgatgttc
    24121 ctgaatgggc agagctttcg gaagagttag tgttagaagg aattgaaaga cctttatttg
    24181 catactttaa aatcccacaa gctaatagtt tagaaaactc atcacctctg ggtgtttctg
    24241 ttttttcaag agcagttgac ttaataaaag aggctgataa gcaatattcg aggattttgt
    24301 gggagtatga ggctacagag atagcaattg atgcagattc tagtatgttc agaagaaacg
    24361 caaatggaga atatgaagtt ccgcatggca agaaacgact gtacaggatg ttagaaatgg
    24421 aggatggaga caataaatgg aatgtgtttt caccagccat aagggattcg agtctttacg
    24481 ctgggcttaa tcaattacta agaaaaatag agttcaattg tggattgtca tacgggatta
    24541 tatcagatgc acaagagatt gaaaaaacag caacagaaat aaaaactagt aagcaacgtt
    24601 tatactctac tgttaaggat atacaaaagt ctttagagga tgccctagag ggattaattt
    24661 attctataga taagtggtct attttggcag gatttactcc tagtttgaag tatgaaacga
    24721 cttttaattg ggacgatagc gtaataatag acaaagattc tgaactctta gcaatgcaac
    24781 aggatgtcgc atctggatta attagacccg aattgtatat tatgaaaaaa tatggagtca
    24841 cagaggaaga ggctttgaaa atgatgcccg acacggggga attagtagag gataatcctt
    24901 ttgacaagga tggtatatag atgttaacac ctaatgagtt acaagaaatt cctaagtatt
    24961 ttattggctt atttcaatct ttagaggact ttataatcgc agatattgca agacgtatag
    25021 caaaagttgg gaacattaca gatatggcag agtggcagtt aataagagca gaagagattg
    25081 gaattgctga taaagttatt aaaaggaaaa ttgctgaaac acttggtatt tcttttgaga
    25141 cagttgacaa gttatttgaa gaaagtgcta taaaatctat tgaaagtgat agtgctttat
    25201 atgaacatgc taaattaaca ccgatacact tgaatagttc tgaagaactt aagagctatg
    25261 tttcttcagc taaagaacaa acgaagggta aactgaagaa tatgacaggt acattaggat
    25321 tttgcactat taaaaaaggt agagttatta gtaagaaact tacagacata tatatagagt
    25381 ctttagactt agcgcaattt caagtcagta caggagtctt ggattataag acagcagtta
    25441 aaaaagcagt taagaggctt gcagatagtg gtttaaggtt tatagactat gagactggtt
    25501 ggacaaatag aatagatgtt gcaactagaa gggctgtgct gactggtgtt aatcagatgt
    25561 cacagaatat taacaataaa gttatagacg atttagatac tgatatagta gaagttacag
    25621 cgcactcggg ggcaaggtgc gagggtgcag gcattaaaaa tcataagaaa tggcagggca
    25681 aatggtatag tttaagtggt aaaagtacga aatacccttc tttaagggct gttacaggct
    25741 ggggacaggg tgatggatta ggaggttgga actgttcgca tcaatttcat gcagttgtac
    25801 ctggtatctc tgttcctgct tacactaaag agcaattaaa aaatatagac cctcccgata
    25861 ttggatataa ggggcgaact tatacgcatt atcaagcgtt gcagtatcag cgcaaaattg
    25921 aaacagctat gaggcagact aagagacaac taattgctta tgagtctgct ggtcttaaag
    25981 atgagtttac aaatgcaagt ataaagttgc aaagacagaa gcaagaatat agagagttta
    26041 gtaaggttgc taaattgagg ttgcagaatg atagacacca ggtgttagga tataataaga
    26101 gcatcagcca aaaggctgtt tatgctggca aaaaggagaa gtaaaatgga tgttgatata
    26161 ttaggtgcta aatatactat agtaaaagat tgcacaaaag ataaagagcc tttattggct
    26221 gaatttgatg gttttatgga tgatactgta aacaagatta taattgctaa aatggagcat
    26281 tcagatgaaa gtttaaagga tttaaacttt tatgaaaaac aagttcttag acatgaagtt
    26341 atacatgctt ttttatctga aagcgggtta aagagtaata gcgattgggc tagaaacgag
    26401 gaaatggttg actattttgc aatacaattt cctaaaatgt taaaagtgtt tatgaaatta
    26461 gacgctttat aaaaaaggag tgatttaaat ggggaaatat agaaagaaac cagttgttat
    26521 tgaggctttt aaatggctag gaagtgcaga acaaaaggaa gaaccgacat ggatagttag
    26581 ggctattgaa agtggtaatg tttggattga acaaagccta ggggaattat ctccacgcat
    26641 gtatataagg acacttgaag gtatacacga ggctaatgta ggtgattaca ttatccaagg
    26701 aataaagggg gaattgtatc catgcaaggc tgatatattt agagaaacat atgaagaagt
    26761 cttatattaa taaggcttat ttttatgctt aaaaatactt aatttattta tatagatgtc
    26821 tactaagcga cgtaaaaagt tagacaatat gtgatgctac cacgtaaaaa gcgtaattgt
    26881 aaacgaaagc aggaggtttt tatgaaaaga gagtttttga aagatttaaa tttagaggac
    26941 gaggttatag aaaaaatcat gtccgagaat gggagggata tagaaaagta taaaaaagaa
    27001 gtagaaaaga aaaaagaaga attggaaagt aaaaatactg aactcgaaac agctaataat
    27061 aaaattaaag acttagaaaa aatagatgtt gagagtataa aaaaagaggc taatgactgg
    27121 aagagtaaag ccgagcaggc tcaaaaagat aaggaattga ttgaaaatca aatgtcagag
    27181 cagacttata acttaaattt agataattat ttaagtaatt ttaagttttc tagtaatctc
    27241 tcaaaagagg ctgtaaaaat aaaaatgaaa gaaaaaggac ttgaatataa ggacggggct
    27301 tttgagggag cagatgatta tataaaagaa ttgcaggcga atgacccagg ggcttttata
    27361 agtggcaata gtgttccaaa ggttgttagt tctagcagtg gagatgctgg agagtctaaa
    27421 gtaagtttaa tggaacaaat gatagcaaaa aatagagaaa tgttgaacat ataggaggta
    27481 gaaaatgagt ttatttgatt caaaaatttt taatggagaa gtgtttggaa agtacgttga
    27541 gactgtacct aatctaaata gggacgaatt aataaagtct ggagcaataa gaagaagggg
    27601 cgatttaaag gctatgtttt cagcccaatc gggagcaaat tatgcaacta ttccaatgct
    27661 tggcttgatt gatggcgacc cagtcaatta cgacggacaa acggatatag tagcaactac
    27721 aacaaagacc ttttcgcagt ctgtaattgt tgttggtaga gctaaatcgt gggtggaaaa
    27781 agactttagc caggacgtaa ccggcggagt tccttttatg gagaatgtag gaaatcaagt
    27841 tgctaaatat tgggataata tagaccaaaa aactttgtta gctgttttgg aaggtatttt
    27901 cgcaatgaca ggggcgaaaa atttagaatt tgttaacaat catacttttg atataacaaa
    27961 agaaacagaa gcagaaaatc aaaaggttaa agctgaaact cttaatagtg caactcaaaa
    28021 agcgtgtgga gataataaag aaaaatttgc acttgttata atgcacagtg ttatatcaac
    28081 taacttagaa aatttaaatc ttgttgctag gttaaaatat actgacccac aaggtattca
    28141 aagagaaatg aagttaggaa cttggggtgg caaattagtc ttaattgatg acaatatgcc
    28201 aactagagag gtcgctgaaa gttcagaggg ttcaaatgat ggatacacag agtatacgac
    28261 atatgtgctt ggggaaaatt ctattgactt cgaggatgtt ggtacaaaag tcacatatga
    28321 aatggataga gttccaggca aaaatggtgg agaagattac ttatatagta gacaaagaaa
    28381 agtatttgcg ccatatggta ttagctttac taaaaaagca gttgcaacag catctccaac
    28441 tgatgcagaa ctgaaaaacg gagcaaattg ggagcttgtg aatgatagtt cgaatgggtc
    28501 taaaacttat ataaaccata aggctatacc tatcgctaga attatttcga gagggtaggc
    28561 tatgaatatg tatatagact atgaatttta tagcactctt gggggagaaa tcccacagga
    28621 caaatttaat aaatatgcaa taagagcaac taagtatata gattataata cttttaatag
    28681 aatagaagaa gttacagaag aaataaaaat agccacttgt gagattgctg acctcatata
    28741 taaaagtaac ctggaaggcg acaaagaaat tcagtctgaa agtattggaa gtcattcagt
    28801 gacttattca gctaacaata aaacgataga gcaaaaggca tatgatattt taaaaatgta
    28861 tttagattca gatttgttgt atcgaggtgt ttagcgtgtt ttttaaagat aatataactt
    28921 tatataataa atattatgat aagtactctg acaaaacttt atacaagagg gcttatttaa
    28981 ttggagtaga ttatcaaggt tctaaaaata tagctgttac agataagggg ttgttaagtg
    29041 ctgatagtgt taaggttata gttccctttt ctgcaaatgc aagagggaag aaatatatag
    29101 accctttcaa atattatgaa ttagatgaaa tcgaaaagga aaaattttat actttcaagg
    29161 ttggcgatat tattgtaaaa gaggtcgtag actttgaaat aactagcgtt aagccttata
    29221 cattaagaga attacaaagt aaatttgatg atgtcagtat tataaaatca gtcattaaat
    29281 gtgattttgg gagcataaga atgcgacata tagagttgga ggcagaatga tatgggatga
    29341 atgtacacgt agatattgac gttgctagaa taatgagaga gcggggtctt gaaagaggcg
    29401 ggaaggcaca aaaatttttt acccatgaag ttagaaggct ttcagactct tatgtcccta
    29461 aacaaagtgg aacgttgaaa aatacagcta gagaagaagt tgacaagata aagtatatac
    29521 agccttacgc taaaatacat tactataata attctgggcg tggttcagaa ggtatggggg
    29581 caggaggcaa aagaggcaag tattgggata aaagaatgtt agcagacaat aaagatgagc
    29641 tgatagggtc tgttgctagg tttattggag gtagtagaga gtgactatag ttgaaagcgt
    29701 tagagagttt ataaaaaaat gcccctactt agatgaattt gcaaaaagta taaatgttga
    29761 atttttagcg gaggaattta cttcttatac aattgagact gttccagctg aaacgattgt
    29821 aaaaaaattt gtaaatggcg actctataaa acaatttgtt tttatatttg caagtagaga
    29881 gtcttatggg tctgatgtta tgcagaatat agaaaatagc cagttttacg aacaatttgc
    29941 ggactggata tatagagaaa atttaaatgg caatttacct gttttagatg gaaataaaga
    30001 gtctatgtca ctagaggtaa gtactacagg ttatccagtt caaacaagta tagacacagc
    30061 acaatatcaa atacaattaa aactaaaata ttttgaaaaa ggagaaatat aaaaatggca
    30121 ggaatagaaa cgatacaaag atataaaatt gcggattatt taggaatttt aggagacgat
    30181 ggacaaaatt cttgggagtt gatgggggca ggatttaata cattagacga gaacccatcc
    30241 gcgcaaagtg actctaaaac atatataaac gatagaaaca gtactagtgc tataaaatct
    30301 taccaaacac aattcccgtt tgagtcggat ttaattaaaa gcgaaaaggc tataatggaa
    30361 ctttataaga taggtagaga ccaggcaact ggcgcagatg ctgaaagaga ttatttaaga
    30421 gtagaattat ttttaccagt tgaaggaaaa gataatactt ttaaggctag aaaatttaga
    30481 gtgtcagttg aagtctctgg gcttgcagga gcaggaggag aaacaattat tgtcagcggg
    30541 aacttaaata gcgttggtaa ttttgtagat ggagaattta acactcagac aaagattttc
    30601 acggaggttg gggaagaagc accaccagct gaaagtttaa agattttaac tgtaaattca
    30661 attgcgggta ctaacacagg ggatacaaaa gtaaccgtta ctccaggtct tgaaagtgga
    30721 aatagttata aatataagac tggggcaagt gtttcagttc cagcattaga tgctgattgt
    30781 tcagttggat atactacttg ggatggtact gaggatataa cagcaaatga aggggataaa
    30841 atattgatag tagaggttaa ttcgggcaat aaagcgaaaa aggcaggaat agctactgtt
    30901 acagtaaaag agtagctata taattgaatt aaaatattta tataaataat taaaaataag
    30961 gagattcaaa agatgttaat taataatata gaattaaaag attttgatat atatgatgct
    31021 gatgaatatg aaaacattga aaaaacaata ggcttgacta ttactgaaac gggaaaagtt
    31081 gagaaagagg caaccaaagg ttatgaaatg attagagata tatgtaattt aatttttaaa
    31141 tgctttgacg atattttcgg ggaaggcaca tcaaataaaa tgtttggaga taagagaaat
    31201 ttaaaggtat gctcaaatgc tttttttgaa ttaatagaag aagttaatag acagaaacaa
    31261 gagggggaag aatttttcgc ctcagctgta tcaaagtata cgccaaaccg agcgcaacgt
    31321 agagctaaaa aataatgaat atgttaatag atgtacttcc cgaaaacgtt gaaattgatg
    31381 gggaagaata taaaattaat acagatttta gaatatcaat tctgtttgaa atgttgatac
    31441 aagatgattc tataagtgac gaggaaaaag ggaaaaatgc actttcttta tattaccctg
    31501 taatacctat aaatactacg gtggctatag ataaaattat atggttttat tcgtgcggga
    31561 aaaaagaaaa tgatattaat gatagttgtc aaagtggtgc gggtggttct agtaaggggc
    31621 aaatatattc gtatgaccat gacgacgagt atatatattc cgcttttcta gggcaatatg
    31681 ggattgactt gcaagacata gaaaaattac actggtggaa atttaaagcg ctatttaaat
    31741 ccttgaaaga agatactgag atagttaaaa ttatgggtta cagggcaatg gatataagtg
    31801 gagatatgcc aaaagcgcaa aaagattttt ataggaaaat gaaaaaaata catgctatac
    31861 cacttcctaa aaatgaggtt gagaaaataa atgaaattga aagagctttg ctaaatggtg
    31921 gagatattag taatttatta taaaataata tactctacac cttccgaatt aatatataat
    31981 tattatataa atattttaat ttggggggat tgttatggga tttaaattta gaaaaagcat
    32041 aaatctaggc ggtggattga agttaaatat taataaaaat agtgttagtg ttagcggtgg
    32101 cgttaaaggt gctagagttt cagttaattc taaagggaaa gctacgacga ctttgggagt
    32161 tcctggaagt ggcttatatt atcaagaaac aactcaacta ggtggaggta aaaagaaaaa
    32221 tggtaagagt gcaggaggct cggagagtag taggcaggct caacagctat taaaaataat
    32281 aaacgattgt gctaatatag ttaatactac aaaagaccct aaaacatttt tttatagata
    32341 taatatgttg ttagataaat catatgcctt agttgccata gaggataact taaacttttc
    32401 tggtcaatct cctagccaga tgctagacag cataatagca aaaaagactg atactataaa
    32461 tgactttttg actagatact acaatgaaat gtctagtaaa ttgaaagaac taaaaactga
    32521 aaaggctcgc atgaacaatg cttataaatt ctctagttta gcaatagatt ataaagagca
    32581 tttaaataat gataataaaa ctaagcttaa taggctatat aaacaactat taaatgatgt
    32641 taaaaaacaa gattaatatt tgtaaagaga aaaatatagt agaagattta aataaaaagc
    32701 acttactttt ttaaaaagta ggtgcttttt taatgcaaat ttataaaaag ggggtgaata
    32761 aatggcagat ggttctatta ttatagatac ccttttaaat accgaagatg cagagaaaca
    32821 acttaataac ttagctgata ctatgaagaa gaaagcaaaa agcgctcttg cgatagctgg
    32881 aattggcgca agtctgggag ctataggaaa acaggctatt gactttgggg atgaatacca
    32941 aaaggcaatg aatggtttcg agtcagcaac gggaaatgcg gaagctaagg caaaaggctt
    33001 tggagaagcg ctacagcagg tctatgcaaa taattttggc gaggacatgg gcgatataag
    33061 cgaagttatg agtttaatat ctcaaaagct tgatgggata gacgctagca atattcaaga
    33121 agttactgaa agtgcgatta tgatgcggga tactttcgac atggatgttg gggaaagttt
    33181 aaatggcgta aactccatga tgcgccaatt tggtattagc gcaaaagagt cgtacaactt
    33241 gattgcacag ggagcacaac aaggattaaa tcagaatgat gacctcggag accagttagc
    33301 agaatattcg acatattacg cacagatggg tttttcagca gaagaaatgt ttaatatgat
    33361 gaaaaacggg gcagagagtg gagtctatca aatagactat ctaaatgacg ctttgaaaga
    33421 atttaatatt agggctaaag acggttcgaa tggaactaag gaagcttttg aggcgctcgg
    33481 ttttaatgct gacgaactaa ctaagaaatt tgcaaatggt ggaagtagtg caaaagaggc
    33541 ttttgttgag gttacaacgg ctttaaataa tttagatgat aatgtattaa aaaatcagat
    33601 aggtgtacaa ctctttggta ctaagtttga ggatttggaa gcggatgcag taacagcgct
    33661 aacaaatatc gaaggaagta taagcagttc aaaagataaa ttagaagaaa ttaataagat
    33721 aaaatacaat agtttcggag aagcgataac aggaattgga aggcaaatgc aggttaattt
    33781 acttcttcca attgcagagg gattgcttcc tgttttaaat gatttggcaa ataaatttgc
    33841 taaggctttt caaaacgaaa atacaaaaaa tgctataaga agtacagcag aaagcattgg
    33901 tgcgttaatt ggaacagttg taaagttaat atctttagca ttaacccctt taatcaatgt
    33961 tataaccttc ttgattcagc atggtagcct tgtagctcct ttgataacag ggattggagt
    34021 tgctttcgct ggattaaaaa ttgctggaat tgtaaggggt atacaaaaaa gctttacaga
    34081 tgcgcaactt gctgtaaggc tgttcgtcac agggatggca gaatcaggaa ctacgttaac
    34141 gctgtatgaa actatagttg gattagttac aaaaaaaata agtttagcgc aagtagcaac
    34201 taatttgtgg aagggtgcat taacggcgct tggtggtccg attggtttaa ctatagtagc
    34261 agtaggggct ttgattgcgg gattcgtatc gctctggaaa aacaatgagg gtttcagaga
    34321 tgccgttata aatatttgga ataatattaa agaagttggg gagaaaatat tcggtcaaat
    34381 tgctaaattc tttactgaaa caataccgca agctatgagt aaatttatag actttgcaaa
    34441 agaaaattgg caaggattgg ctctattact tgttaatccc ttcattggtg gttttaaatt
    34501 aatctatgat aattgcgaag gttttagaaa tactataaat aaattattta acagcattaa
    34561 agatgcttta agcaaaacaa tgtcttttat taagagtaac tggcaaggct tattattaat
    34621 tttagtaaat ccgttcattg gagcatttaa attaatttac gataattgtg aaggttttag
    34681 aaacactgta aattctatat ttaacaaaat tgtaggcatc tttaaaaatg ttttaagttt
    34741 tattaaaaat aactggaaaa gtataggaag tatgttatta agcccttttg taaatggatt
    34801 taaagctatt tataatagtt gtgtaagttt taaaaataag gtttttagtt tcttttctag
    34861 tgttgtaaaa ggctttgtta cctttggaaa aaacataata gctgggataa taaatgggct
    34921 ttcgagcggt atcggtttgg ttgtagatac tgtaaaaaat atcggtttaa ctgtagttaa
    34981 tacatttaaa aaagtactaa gaatacattc accgtctaaa gttacaacag aacttggaga
    35041 atttgcaggc attggatttg ctaatggtat aaaaaataca agcaaagatg tagtagaggc
    35101 aacaaaagaa ttaataagtg ctatggaaaa aaatcttgag accaacaaga gtaaatttga
    35161 aaaaatttgc gaggctataa ctacagcatt aaaaaatcaa tatgaaagtc aaaaagatat
    35221 acaaataaaa gcgcttgatg aaagattaaa agttgaggaa aaggcatcga atgatagatt
    35281 aaaggtctat gaaaaagaat acaatgaaaa attaaaatat ttagatacag aaacaaacga
    35341 aaaaactaat gctatacaag aacaaattga cgctatagac aagcaaattg aagaagaaca
    35401 aaaagcagaa gaagagaagg cgtacaatga aaaaataagc gacctcaata ggaaactagc
    35461 aacagctaaa aatcaaaaag aacgagaaaa aatacaaaaa gaaatagcag aagcacaagc
    35521 agatagacaa aagaaattat tagatgaaca aaggcaacaa gaaaaaaaca gattaaaaga
    35581 acagattgaa aatataaaaa cagaagctag taacaaaaaa gagcagtaca aagaagagta
    35641 cgacaatcaa aaggaagcag aaagtaaaaa acttgaatta ataaaagagt ctaatgctag
    35701 tcaaaaagaa gaaattgaaa aatattttag tgaactttta gaagagacaa atattcaaaa
    35761 tgaagcgaga aggctacttt tacaaaaaaa ttcagaggaa attataaaac ttttaagcga
    35821 atataatccg cattggcagg atgcaggaca aagtttagca gatagtttgc taaatggagt
    35881 taactctaaa aagcaatcta tccaggaggc tgtaaaggaa gctatcaact taaaagagat
    35941 tataccaact caagaaaaag agcttgatag attaaagaaa aagttagaag aatatgagaa
    36001 actaaaagaa aatgctagta cttctagttc aagtgggggg gatttagaca cttccgctca
    36061 agattcttta gttaattcgg gttcaagtgg attgaatact ggagaactag aaaaatacgc
    36121 ggatggtgta gacgatatta aagattcagt tgaagggtta gaccttgtaa gtgatgaact
    36181 cgtaacagga acagtcccta aaattgagga ttcagctggt aaattatctg aaagcataaa
    36241 aaatggattc agcggtatag gtgatttttt cggcggaatt gaaaaatggt taagagatac
    36301 agacaaaaat ataaatgaat ggttagctag tgcaaagaac tctattgggg attttttctc
    36361 taaagtaaaa gataaattca aagaaggaat tgaaagtata ggcggatttt tttgtggtct
    36421 tggaaataaa ataagtgaag gatttgcgag agctggagac tttttcgcag ggcttggaga
    36481 caaggcgcaa caaggatttg caagtataaa agacaaagct gtagagattt tcagcggtct
    36541 tggcaattgg ttcaaagaaa cttggtcgaa aattggagat gcgattggaa acggattaaa
    36601 aagcggttta gactttttta ctagcacagt accagagtgg tttgcgggta ttggcgataa
    36661 aataaaagaa gaatgtggga aaataggaga aaaaatatca agtttcttta atgagacaat
    36721 gccagctata ataaatagca ttgccgaatg gtttaaacaa attccataca acattggctt
    36781 tatagttggg tctattgcta gattctttgt tgatttaggg actaatctat atgcatgggc
    36841 aacagaaaca ttgccaggga taattaacag tattatagaa tggtttgtaa gccttccgga
    36901 aaaaattggg gagtttctta gtgctacact acaaaatatt caaacttggg gcgaaaattt
    36961 caaggtttct gtttcagaat tttttagtac gatttgggaa aatataacac agttctttac
    37021 agaactaccc gagaaaattt cggaatggtt taatacagta gtagaaacag tcagtagctg
    37081 gggcgagaat ttaaagcagt tagcaacaga tatttttaat caatttgttg acaatgtatt
    37141 aattccagtt tcacaacttc caggcaagat atgggaaaag ttcgcagata ttattaacaa
    37201 agttaatgaa tggggttcta atctattaca aaaaggacta gatatagcag gtaaattttt
    37261 agaaaatgta atgaaattct tttcagaact tccaaaaaaa ataaaagaaa aattagacga
    37321 tataataaga aaagttacag attggggaac taatctaata aataaagctc ttgaaattgc
    37381 aaaaaacttt tttaataata tttacaatac agttagccaa ttaccaggaa aatttaagga
    37441 atggctagac aatatcattt caaatgtcat aagttggggg actaacttag tcaaacaagc
    37501 tggagaagct ggaaaaaata tggcaaatgc tgttaaggat gctttgaagg atttgcctag
    37561 cagaattatg agcatcggga aagatgtcgt aagaggcttg tgggaaggta taacgggcat
    37621 gggtggctgg ttaaaagaca aagtctttga ctttgctggt gacgttataa acggatttaa
    37681 ggatggtttt ggagtacatt cgccttctat aattatgcgt gatttgattg gtagaaacct
    37741 tgtaagagat gttggcgttg gaatagatgt agaaacacca ggattgaaag aaaaaataga
    37801 gaaaaatata tctgaactta caggtaaatt aaaagctaca gttaattttg aaacgtctaa
    37861 aatgcaagct aatatagttg caagtacaga ttttaaagct gaaaaagaaa catctatgat
    37921 gagtagcaat aagactgatg aagctaatga aaatcaagca ggtattaaac ttaatataga
    37981 aaactttgta aataatagac aacaagacat agaaaatctt tttgatgaaa tattgttcct
    38041 agcaaaaaga aaaggagctt tataaaatgc tccttttctt ttttatattt ccttttatct
    38101 ttttttgaaa agactatagt aaaaaatata actacaaaaa acggagttat gtctaaaaaa
    38161 taataaatga gaggagggca taaagctatg tttttcgttt atgacggtcg agacagtaga
    38221 gaatttggct tgaaaatata caatataaat gacttatcag ctccgcaaat ggaagttgaa
    38281 agagtgggtg tgccaggtag ggatggagat ttactactaa aaaaaggctt tggaaatttt
    38341 acgcttacaa tagagtgcga tatagatgca agacaaagca acatagaaga agttgcaaca
    38401 gaaataaaaa aatggctaca gggcgacata tcatataaaa aacttttttt aagtaatagt
    38461 gacttttatt atctagcaag ctgtaacaat aaactagata taactagaaa ttttaaaaac
    38521 tttgcatcat gtctcttaac attcgattgc taccctttta gatacgcaga agaaaaaatt
    38581 ataagtttaa atgtattaaa tctaaagagt gctactataa caaatttcta tagagagtca
    38641 aaacctgttc tctatataga aggagcaggg gacataagta taaaaataaa tactcaaagc
    38701 atagtgctaa gaggagtatc agaaaatgga attttaagtg acttgataat agatagcgag
    38761 cgaatgaatg tatatagaat aaacaaagaa aataacatta ttgtaaatga aaataataag
    38821 ctttttagtg actttcctat atttgaagaa ggagaaaatc ggattagctg ggaaggagat
    38881 ataaaaagta taaaaataaa tcctagatgg aatattttat aaaattttta aattaaaatt
    38941 cggagggagg gattaattat tcaaaaaggg aaattaattt tatatgaaga aaaagaaata
    39001 gattttagac atttagggct gggtgtcctt acaaatgtaa taaatgacca tgtgagagag
    39061 gaagaaaatg gagttttcga gttagaattt actatctacg aagactcttt tttatttaaa
    39121 gaaataaaaa ctgatagatt agtaaaagct gacgcgtctc cagatttcaa aaatcagctt
    39181 ttcagaattt attatatatc aaaaaatcta ggtggatata ttaacataaa agcgcaacat
    39241 attaaatacg atttacttaa taacttcata gaaagtttaa atttgaacga tgttggatgc
    39301 gaagaagcgt tagaaaaggt ctttcgtgct tgtgaagaag agaaccgatt tagagggcat
    39361 tcagatatca aggtaaggaa tactattaat atcgaaatgc aaagccctta cagcgcaatc
    39421 tgcgagggcg aaaattctct aattcaaaaa tttgaaccaa ctgcgaaact tttctttgat
    39481 aatttcgacg cttatttaaa ttatcaaaga ggagaaagta aaaatgtatt acttgcatat
    39541 agaaaaaata taacagggct tgaagcggaa tatgatacac aagatattgt aactaaaata
    39601 tatccttttg caacttacga ggatgaaatg attacattaa cagaaaaata cattgttagt
    39661 cctaatctga ataaatatgc tactcaaaag attgttgcta tagattttag ttctgatgag
    39721 gtcagtgttg aagaagaact aagagaaaaa tgcaaagatt attttaaata caatcaagtt
    39781 gatttaccaa aaattttata taaggtcaac tttgtagatt tgtctacaac tgtaaattat
    39841 aaagattata agatgctaga aacagtcaat ctaggcgatg aagtaataat acgagatttt
    39901 aatttaaaca tcaatgcaac tgctagagta gttaaaacag actatagccc aataaataaa
    39961 aaatactata gtattgaagt tggcgattta ataaatcatt tagacttttt aaacaacaag
    40021 tttaataata tagaaaataa aatagataca gtaaaaaaag ctattgataa tgtcaaagta
    40081 gatgattctg agtttccaga tactttacca aatatacccg ttttgacagc agaagggtta
    40141 ttcgctttca tcaatctgac atggacattt acaaataagt cttattacaa atatgaagtt
    40201 tatgcgtcgc aaattaaaga ctttgtacca gatacaatta attttacaaa tcgaattttt
    40261 acagggcaag caagtgctta cgttcatcaa gctgaacctt ttcaaacttg gtattttaga
    40321 gtaagagcag taaactcaca tggaaatgtt acagagtttt ctaatcaagt tgaagcacag
    40381 actacaaaaa tgtcggatgg agctacatgg atacaagagg gagcaatcgc ggatgctctt
    40441 attgggcaac taaaacttga cagaggttgg tttggacagt taaaaggttc atatataaat
    40501 gcgagagaat tggtggttga aaatgataac ggagttaaaa cgatggaagt agatagtttt
    40561 ggagatgttc atttcaaccc aaacactctt aaagttgctt ttaataatat aagtccctac
    40621 gtccttattg atgatgaagg gcttaagctt ataagcggta agggatttac aaaaatgacc
    40681 gactcgggac tatatacaaa attctatact gattcagatg taacggagtc gtggtatttg
    40741 caagaaagtg gatacacata ctttgactat aaagattcaa aaatcataat agacttacca
    40801 aaaaaatttg caggtaaaaa ttttaaagca acttgtgcga taaggtctac atcgacgaat
    40861 gccagttgct taagttattt cagtacatcg gcgacagtaa aaaaaagtga tacaaatcca
    40921 caattagagc tttctgcatc tgtgcgaggt gtgtcttacg aaatgtcagg aaatcaaaca
    40981 ataggctact ggctcggaat gaattttttt tcagaaggat ttattacagt acaatactat
    41041 gtttacgctt aaaattttca aaaaaggaga agaagtatgg ttactatttt ttacagtaag
    41101 agaactggag aaatatataa ttgcataaaa tcagaggtag agcaggatta tacttgtttt
    41161 gcagataggg aagaggatta tagacaaata ttagataaaa tagttgttga ggatattcca
    41221 gaaattttaa atcctcgtga ttataaaatt gaaaacggaa aatttgtatt aaaggaaaaa
    41281 gaaataataa aagaatttac tccgaaaatg gtagagtaaa aggagtgata aatttgagag
    41341 atagaattta tacagtagat attaatacta aaagttatca agttgcaaaa tataagcaat
    41401 atgataatgg gatagagttt aagattaatt tattagaaaa taatatagaa aaagatttaa
    41461 caggatatac agctatagca aattttcaaa gaccagatag gaaaatagtt tatcagagct
    41521 gtactataga aaattctatt gtaacaactc tgatagaaaa taacataaca gcagttgcgg
    41581 gggatgtaac agtagagttt actttctaca aagatgattt agttgttact actttttctt
    41641 taaagattaa tatagagaaa agtatagata aaaacagtat tacagaagag ccgaaatggg
    41701 attatatcag cgttacaata aatcaagtaa aagaagtcgt cgaaggcata gaagaaataa
    41761 aagaaacaga agaagcaaga aaagaggctg agattgaaag ggtcaaggag tttaatagta
    41821 ttaaagaaac tttcgactct aaagttacag aagttacgga cgctaaaaat actatgatta
    41881 gtgacgttaa tactactaaa gatactttaa caagcgaagt tacagatgcc aaaacagact
    41941 taattagtgt agttactaca gctaaagaaa atatgattaa tgaagtaagt gcagttaaaa
    42001 cagaacttga aacagcagaa gctgaaaggg tcgaagaatt taataatata aaagaaactt
    42061 tcgactctaa agttacagaa gttacggacg ctaaaaatac tatgattagt gacgttaata
    42121 ctactaaagg tactttaaca agcgaagtta cagatgccaa aacagactta at
//
LOCUS       DAKBNN010000010        40465 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000010 DAKBNN010000000
VERSION     DAKBNN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.010"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            49..1245
                     /locus_tag="OUB02_000575"
     CDS             49..1245
                     /locus_tag="OUB02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCT8884373.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1338..1862
                     /locus_tag="OUB02_000576"
     CDS             1338..1862
                     /locus_tag="OUB02_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCT8884374.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1887..2063
                     /gene="rpmF"
                     /locus_tag="OUB02_000577"
     CDS             1887..2063
                     /gene="rpmF"
                     /locus_tag="OUB02_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCT8884375.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2270..2827
                     /gene="fapR"
                     /locus_tag="OUB02_000578"
     CDS             2270..2827
                     /gene="fapR"
                     /locus_tag="OUB02_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCT8884376.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2838..3860
                     /gene="plsX"
                     /locus_tag="OUB02_000579"
     CDS             2838..3860
                     /gene="plsX"
                     /locus_tag="OUB02_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCT8884377.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3886..4872
                     /locus_tag="OUB02_000580"
     CDS             3886..4872
                     /locus_tag="OUB02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCT8884378.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4981..5910
                     /gene="fabK"
                     /locus_tag="OUB02_000581"
     CDS             4981..5910
                     /gene="fabK"
                     /locus_tag="OUB02_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HCT8884379.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5926..6876
                     /gene="fabD"
                     /locus_tag="OUB02_000582"
     CDS             5926..6876
                     /gene="fabD"
                     /locus_tag="OUB02_000582"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCT8884380.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6876..7625
                     /gene="fabG"
                     /locus_tag="OUB02_000583"
     CDS             6876..7625
                     /gene="fabG"
                     /locus_tag="OUB02_000583"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCT8884381.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7679..7903
                     /gene="acpP"
                     /locus_tag="OUB02_000584"
     CDS             7679..7903
                     /gene="acpP"
                     /locus_tag="OUB02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCT8884382.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7932..9170
                     /gene="fabF"
                     /locus_tag="OUB02_000585"
     CDS             7932..9170
                     /gene="fabF"
                     /locus_tag="OUB02_000585"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCT8884383.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9396..11114
                     /locus_tag="OUB02_000586"
     CDS             9396..11114
                     /locus_tag="OUB02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8884384.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11145..13145
                     /locus_tag="OUB02_000587"
     CDS             11145..13145
                     /locus_tag="OUB02_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8884385.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKNIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13496..13741
                     /locus_tag="OUB02_000588"
     CDS             13496..13741
                     /locus_tag="OUB02_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HCT8884386.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13776..14501
                     /locus_tag="OUB02_000589"
     CDS             13776..14501
                     /locus_tag="OUB02_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCT8884387.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGMIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14523..15836
                     /locus_tag="OUB02_000590"
     CDS             14523..15836
                     /locus_tag="OUB02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8884388.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            16042..16581
                     /locus_tag="OUB02_000591"
     CDS             16042..16581
                     /locus_tag="OUB02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8884389.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16670..18655
                     /locus_tag="OUB02_000592"
     CDS             16670..18655
                     /locus_tag="OUB02_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCT8884390.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18879..19847
                     /gene="spoIIIAA"
                     /locus_tag="OUB02_000593"
     CDS             18879..19847
                     /gene="spoIIIAA"
                     /locus_tag="OUB02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HCT8884391.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGSYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19837..20358
                     /locus_tag="OUB02_000594"
     CDS             19837..20358
                     /locus_tag="OUB02_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HCT8884392.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20375..20572
                     /gene="spoIIIAC"
                     /locus_tag="OUB02_000595"
     CDS             20375..20572
                     /gene="spoIIIAC"
                     /locus_tag="OUB02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HCT8884393.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20588..20974
                     /gene="spoIIIAD"
                     /locus_tag="OUB02_000596"
     CDS             20588..20974
                     /gene="spoIIIAD"
                     /locus_tag="OUB02_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HCT8884394.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21059..22264
                     /gene="spoIIIAE"
                     /locus_tag="OUB02_000597"
     CDS             21059..22264
                     /gene="spoIIIAE"
                     /locus_tag="OUB02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HCT8884395.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22324..22941
                     /locus_tag="OUB02_000598"
     CDS             22324..22941
                     /locus_tag="OUB02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HCT8884396.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22953..23507
                     /locus_tag="OUB02_000599"
     CDS             22953..23507
                     /locus_tag="OUB02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HCT8884397.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23534..24223
                     /locus_tag="OUB02_000600"
     CDS             23534..24223
                     /locus_tag="OUB02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HCT8884398.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24333..24695
                     /locus_tag="OUB02_000601"
     CDS             24333..24695
                     /locus_tag="OUB02_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCT8884399.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24780..25286
                     /gene="nusB"
                     /locus_tag="OUB02_000602"
     CDS             24780..25286
                     /gene="nusB"
                     /locus_tag="OUB02_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCT8884400.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25270..26340
                     /locus_tag="OUB02_000603"
     CDS             25270..26340
                     /locus_tag="OUB02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HCT8884401.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVILNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26337..27542
                     /locus_tag="OUB02_000604"
     CDS             26337..27542
                     /locus_tag="OUB02_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCT8884402.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKVKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27544..27759
                     /gene="xseB"
                     /locus_tag="OUB02_000605"
     CDS             27544..27759
                     /gene="xseB"
                     /locus_tag="OUB02_000605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCT8884403.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27762..28649
                     /locus_tag="OUB02_000606"
     CDS             27762..28649
                     /locus_tag="OUB02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCT8884404.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28679..29116
                     /locus_tag="OUB02_000607"
     CDS             28679..29116
                     /locus_tag="OUB02_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HCT8884405.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29150..31015
                     /locus_tag="OUB02_000608"
     CDS             29150..31015
                     /locus_tag="OUB02_000608"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCT8884406.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31148..31960
                     /locus_tag="OUB02_000609"
     CDS             31148..31960
                     /locus_tag="OUB02_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HCT8884407.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31980..33659
                     /gene="recN"
                     /locus_tag="OUB02_000610"
     CDS             31980..33659
                     /gene="recN"
                     /locus_tag="OUB02_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCT8884408.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKKVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33874..34740
                     /locus_tag="OUB02_000611"
     CDS             33874..34740
                     /locus_tag="OUB02_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884409.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKYGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKFTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34833..35324)
                     /locus_tag="OUB02_000612"
     CDS             complement(34833..35324)
                     /locus_tag="OUB02_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8884410.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35385..35825)
                     /locus_tag="OUB02_000613"
     CDS             complement(35385..35825)
                     /locus_tag="OUB02_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8884411.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35986..37044
                     /locus_tag="OUB02_000614"
     CDS             35986..37044
                     /locus_tag="OUB02_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HCT8884412.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFAI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37186..38010
                     /gene="spo0A"
                     /locus_tag="OUB02_000615"
     CDS             37186..38010
                     /gene="spo0A"
                     /locus_tag="OUB02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HCT8884413.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38423..39544
                     /locus_tag="OUB02_000616"
     CDS             38423..39544
                     /locus_tag="OUB02_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884414.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39562..40437
                     /locus_tag="OUB02_000617"
     CDS             39562..40437
                     /locus_tag="OUB02_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HCT8884415.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
BASE COUNT        16413 a         4109 c         7604 g        12339 t
ORIGIN      
        1 aaaatttatt acatattatt tataaaaaaa tctaaggagg aaataataat gaaaatatta
       61 gtattaaact gtgggagttc ttcattaaaa tatcaattaa tagatatgaa taatgaagaa
      121 gttttatgta taggattggt tgaaagaata ggaatagaag gttctatatt aaaacatgaa
      181 aaagcaggta gagatgataa atacgttgtt gaacaaccaa tgaaggacca caaagatgca
      241 atagcattag ttttagaagc tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa
      301 gaaatagatg cagtaggaca tagagttgta catgctggag aaaaatttgc aacttcagta
      361 gtaataactc cagaagtaga agaagcttta aaagaatgta tagatttagc tccacttcat
      421 aatccagcaa acataatggg aatagatgct tgtaaagcta tacttccaga tgtaccaatg
      481 gtaggtgtat ttgatactgc tttccatcaa acaatgccta aatcttcata cttatatggt
      541 ttacctcatg agctatatac taaatatggg gtaagaagat atggattcca cggaacttct
      601 cataattatg tatctcaaag agcagcagag atactaggaa aagatataaa agatttaaaa
      661 atagtaactt gtcacttagg aaatggtgct tctatagctg ctgttgatgg tggaaaatgt
      721 gtagatacat ctatgggatt cactccatta gaaggattaa taatgggaac tagatgtgga
      781 gatatagacc cagctatatt accattctta atgagaaaag aaggtttaga tgctgatgga
      841 ttggataaat taatgaataa agaatctgga gtatatggaa tgactggaat ttctagtgac
      901 ttcagagata tagaagatgc agctaaaaat ggagacgaaa gagctcaagc tacattagaa
      961 gcgtatgtta aaaaagtaca aaaatatata ggcgcttatg ctgctgaaat gaatggattg
     1021 gatgttgtag tatttactgc aggcgttggt gaaaatggaa aggctataag agcagatatt
     1081 gcttctaaca tggagttttt aggaatgaaa ttagataaag aagctaatga tgttagagga
     1141 aaagaaacag taatatctac tgctgattct aaagtaaaaa tgcttttaat accaactaat
     1201 gaagaattaa tgatagctag agatacatta agattggtaa aataattaaa ttaaacctat
     1261 gcctatcaag taaaaatact tgacaaacaa atgctagatt tgtataataa atttggtgat
     1321 atgttgaggt gaacttaatg aaagttagca tagaaaaaat aaatagaaaa gaaactgaca
     1381 aaatagactt gaatttttgt gaaaaaattg atactattag ttattgtgat gagatttaca
     1441 aattagtatc acctgtaaat ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc
     1501 tagatataga tgtcaatttt acaattgttg acaattgttc aagatgtctt aaagaagtag
     1561 aaataccact agagtattct atacaaggtt ttttagtaaa agaagaagat tatgatgaag
     1621 atgagtttga ggaatttgat ccttttatat tcgatggtga agaaatcgac cttattgata
     1681 taatagaaca gacattagat tttaatgtac ctcataaagt tctttgtagt gaaaactgta
     1741 aaggtctttg tcaagtatgc ggggcaaact taaatgaaga agagtgttct tgcagtgaaa
     1801 ttacaaacga tgaggaatac atagatcctc gttttgctaa attaaaagat ttatttaatt
     1861 aaattttgac taaggaggtg gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa
     1921 acacaaaaat gagaagagca gctaactcaa agatggaagc aacaggattt gtaagttgcc
     1981 cacaatgtca tgagccaaaa ttaccacata gagtgtgtcc agactgtgga tattataaag
     2041 gtaaagaagt tgtgtctaag taatagcttc taaaaagcat gtagagtata ctctacatgt
     2101 tttttttgta catataaaaa aataagatga tttactagag aaaataagtg gattttatgc
     2161 atattaaaaa atagaaaaat aataatataa gtattgaaaa tttttgtgtg ttactatata
     2221 ctactattag tagctggtac taattacagc tataaaattt ggagggaaca tgaaaaagaa
     2281 aagtaaagcc caaagacaaa aagagcttat agatatgcta aagacagatc ctttttatac
     2341 tgatgaagaa ttatcaagtc tgtttgatgt aagtatacag accataagat tagatagaat
     2401 gagtctaaac ataccagagc taagagaaag agtcaaatca atagcagaaa ctcagagttc
     2461 aaaagtaaag actttaggag taaaggaaat tacaggagaa atcattgatt tatctgtagg
     2521 gcgattaggt atatctatgt tagaagttac acaggacatg atttactcta agactaatac
     2581 tttaaaagat acgtatattt tttctctagc agattcattg gcaatggcta taatagatgc
     2641 acctaaggtt attatgagag ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga
     2701 tagacttata gcaaaagcag aagtgtatag aaatatagat aaaaagcatt atgtaaaagt
     2761 tgttatcaac aataaagccc aagagcaaat atttagagga aagtttatct ttgaagaatt
     2821 agattaggag ggaaattatg aaaatagtaa ttgatggaat gggtggagat aatgcaccta
     2881 aatctaatgt agaaggcgca gtaaatgcta taaaagaata tcaggttgat ttaataatca
     2941 caggagataa agatttactt gaaaaggaat tttcaaatta tgaatttgat agaaataaat
     3001 tagagatagt tcatactact gagattatag aaaacgaaga taaaccagta aaggctataa
     3061 gaagtaaaaa agattcatct atggtagtag cattaaatct tgtaaaagaa ggaaaggcag
     3121 atgctataat atcagctgga aatacaggag ccttacttgc tggtggatta tttgttgttg
     3181 gtagaataaa aggaatcgat agaccttgct tatgttcagc aataccaaat gtaaagcgag
     3241 gaatgacact tatagcagac tgtggcgcaa atgcagattg taaacctaag aatctagttg
     3301 aatttgcagc tatgagtaat atttattcaa gaaaagtttt aggtcttgaa aatcctaagg
     3361 tagcattagc aaatgttgga ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat
     3421 atgaagaaat taaaaaatta gatttaaatt ttatagggaa tgtagaagct agagaagtta
     3481 taaatgcata cacagatata atcatatgtg atggatttac aggtaatata ttacttaaat
     3541 ctgctgaagg tgtagcactt tcagttatga gtcttataaa ggaaactttt atggccagta
     3601 caaaaagtaa aataggagca ttacttataa aggatgattt aagaaaatta aaaagtttta
     3661 tagattattc tgagtatggg ggagctccac ttttaggtct aaatggagga gttataaaag
     3721 ctcatggtag ttctgatgct aaagctataa aaaatgctat aaatcaagga ataaaatttt
     3781 caaagggaaa agtcgttgaa gatattaacc aatttataag caaatacaat gaagaaaata
     3841 aaaataacga agatgaataa actcgttttg gaggtttaat atataatgaa tacaaaggct
     3901 ggaatattag gagtaggaag ttatctacct gagcaatcat atgataattt tcacttcgaa
     3961 aaaataatgg atacatcaga tgagtggata agtactagaa ctggtattaa ggaaagaaga
     4021 tttgccaaag agtctgaagc aacttctgac ttagcatcta aagctgcctt aaaagctata
     4081 gaatgtgcaa agttaaacgt agaggatata gagctgatta tattagctac aattactcct
     4141 gatatgtctt tgccttcaac tgcatgtata gtacaagatg caataggagc agtgaatgct
     4201 actgcatttg atatatcagc agcttgttct ggatttgtat atggagtaac tattgcaaag
     4261 caatttgtgg aaacaggttg ctataaaaat gtgcttgtaa taggagcaga aacttgttct
     4321 aagtttttaa attatgatga tagaacaaca gcagtattgt ttggagatgg agctggagca
     4381 gctgttattg gccctgtaaa tgagggtgga atactgtcta cacatatggg ctctgatggt
     4441 aaaggaaaag attgtttaaa agtacctgct ggaggttcaa gacttaaggc aagtaaagag
     4501 actgtagaag ctaatctaca tacaatagaa atggcaggta gtgatgtgtt taaatttgca
     4561 gttagaaaga tggcagaaac ttctttaaga gctcttgaaa aagcaaatct aaatacaact
     4621 gatatagact acttagtgcc acatcaagct aatataagaa tcatacaagc ttcttctaag
     4681 agattagagc ttgatatgaa aaaagtttat gtaaatatag acaaatatgg aaatatgtct
     4741 gctgcatcta taccagtagc attagatgaa gcttatagag aaggtaaaat taaaaagggt
     4801 gataatgtag ttttagttgg ctttggtgga ggtctaactt ggggggcttc agttgtcaag
     4861 tggactttat agtataaaat atctaaaatg atttaattaa caaaattaaa cttatacaca
     4921 aaataaagtt gattaataat aaaaagttta cttttatgat ttcaaatgaa ggagaattgt
     4981 atgaataaaa tttgcaaaat attaaatata aaatacccag ttatccaagg gggaatggca
     5041 tgggtagcta ctgcatcatt agcaagtgct gtatctaatg caggaggact tggcataata
     5101 gcagcaggaa acgcaccaaa agaagctata aagaaagaaa ttgttgagtg taaaaaatta
     5161 acagataaac cttttggagt aaatgtaatg cttatgtcgc catttgttga tgatataatt
     5221 gatttgatta tagaagaaaa agttcaagtt attactactg gtgctggaaa tcctgcaaag
     5281 tatatggata gattaaagga agctggaaca aaggttattc ctgtagtacc tacaatagct
     5341 ttggcacaaa gaatggaaaa gctaggagct acagcagtaa tagcagaagg tactgaaggt
     5401 ggaggacata taggagaact tactactatg gtcttagttc cacaagttgc tgatgctgta
     5461 aacatacctg taatagctgc tggaggaatt gtagatggta gaggaattgc agcatcattt
     5521 gcattaggtg ccagtgcagt tcaagtagga actagattta tttgcagtga agagtgttct
     5581 gtccattcaa actataaaaa cttagtacta aaagcaaaag atagagatgc aattgtaaca
     5641 ggaagaagta ctggtcatcc agtaagaaca ttaaaaaata aactatcaaa agaattttta
     5701 aagatggaac aaaatggagc tactcctgaa gaactggata aaaaaggtac aggagcttta
     5761 agatttgcaa cagtagatgg agacatagaa aaaggttcat ttatggcagg tcaaagtgct
     5821 gctatggtaa aagaaataac accttgtaag gaaattatag aggctatggt aaatcaagca
     5881 agagagatta tgccagcaat agaactgtaa atcaggagga gcaagatggg aaaagttgca
     5941 ctagtattcc ctggtcaagg agctcaatat gtaggtatgg ccaaggattt atatgaaaat
     6001 aatgctgttg ctaaaagtgt aatagatgag gctagtgatg ccttaaatat ggatttaaaa
     6061 aaccttatgt ttaatggaaa tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgcc
     6121 atggttacac atagtgtagc agtattaaaa gcggtacaag ctcaaataga tttaaaatat
     6181 aatgcatgct taggattatc cttaggagag tatagtgcgt tggttgcagc agatgcaatt
     6241 gactttaaag atgctgtatg tttagttaaa aaaagaggta agtttatgca ggaaacagtt
     6301 ccagcaggtg ttggagctat ggcagcagtt ttaggtttag atagaaatgt acttgaaaat
     6361 gtggttaaag atatacaagg aggtattgta gaagtagcaa attacaattc tcctggacaa
     6421 atagttatat ctggtgaaaa tgaaaagata gaagaagcaa tggtaaaatg caaagaagca
     6481 ggagcaaaaa gggcagttaa attaaatgta agtggacctt ttcactcatc aatgttaaaa
     6541 gaagctggtg taaaattggc cagtgaatta gaaaaagtta acataactaa acctaaagtt
     6601 gatgtggtgg caaatgtaaa tgcagattac tataaaaacg aaggtaaaga cctgcttata
     6661 aaacaagtaa gttcatctgt tctttgggaa gattctatag aaagactttt aaatgatgga
     6721 tatgatactt ttatagaaat gggacctgga aaaactttaa aagcatttat taagaagata
     6781 gcttccaata aaaaagcaaa tgtcaatatt tataatatag atggtataga ttctcttaat
     6841 gaatttgtac aaaattatag aaatggagag atataatgat aaatcttaca ggacaagtag
     6901 cagttgttac tggtggctct aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt
     6961 ttggagctga tgtagtaatc aattatactt ctaaagaaga tgaagcacta aaaactaaaa
     7021 atgaaataga aagtatgggg gtaaagtgta cctctataaa atgtgatgta tctaaatttg
     7081 atgaagtgaa tcaaatgata gattctgttg taagcgaatt tggaaaaatt gatatattgg
     7141 ttaataatgc aggcataact aaagatggtc tgcttatgag aatgaaagaa gaagattttg
     7201 atagagttat agatataaac ttaaaaggtg tctttaattg tacaaaagca gttactaaac
     7261 ctatgatgaa aaagaagtat ggaagaataa taaatatgac ttcagtagtt ggaattatgg
     7321 gtaatgcagg gcaaactaat tattgtgcat caaaagcagg tgtaattgga tttacaaaag
     7381 cttctgcaag agagttagca tcaagaaaca taaatataaa tgcagtagca cctggattta
     7441 tagaaacaga tatgactaaa gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca
     7501 taccaaagaa atcttatggt aaaccagaag atgtagccaa tgccgtagca tttttagtta
     7561 gtgacatgtc aagttatata acaggacaag taataaatgt agatggtgga atggtaatgc
     7621 aataataatt taaaaagtag agaaatctat taaaataaaa aattttagga ggtacacaat
     7681 gtttaataaa gttgtagaaa taataataga gcaattagga gtagaagata aagaaataac
     7741 tatggaaaca tcattaatga aggatttaga agcagattca ttagatgcag tagaaataat
     7801 aatggcactt gaagatgaat ttggtataga aataccagat acagaagcag aaaacttcaa
     7861 atctatagga gatatagtta agtatataga agctaataaa tagtcgagct ttaaaaaagt
     7921 ggaggtatcc gatgaataaa agagtagtaa taacaggact tggatgtgtt acgccgttgg
     7981 gaacaggaaa agaagagttc tggagtaata taaaatctgg agtatctggt atagataaaa
     8041 taactaattt tgatgctagc acatatcaaa ctcagatagc aggagaagtt aagaattttc
     8101 atccagaaga atatataagt aaaaaagaat tgaagagatt agataaattt gctcaatttg
     8161 caatagtatc tgctaaatta gcagttgaag atgcaaatct tgatttagat aaagttgata
     8221 gagaacgttt tggtgttata ataggttctg gaataggtgg agttgaagcc atagaaactc
     8281 aacataaaat tcttttggaa aaaggaaata aaagagtaag ttcacttttt gtacctatga
     8341 tgataggaaa tatggcagct ggtcaagttt caatattttt aggagcaaaa gggcctaaca
     8401 ctaatgtatg tacagcttgt gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa
     8461 ttcaaagagg tgatgcagac attatggtag cagggggttc tgaagctgct gtaacaggtc
     8521 ttgcttttgc tggattctgt aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa
     8581 ctgcatcaag accatttgat aaagacagag atggttttgt aatgggagaa ggagcaggta
     8641 ttgttatctt agaagactta gaacatgctt tagcaagagg cgctaaaata tatgcagaag
     8701 tagttggata tggtttaact gctgatgcat accatatgac aactccagct gaaaatggag
     8761 agggagcagc taggtctatg aatatggctt tgaaagatgg aaatgttcca ttagaagaag
     8821 tagattatat aaatgctcat ggaacttcaa cttattacaa tgatttatat gaaactatgg
     8881 ctataaaaac tgtatttgga gaaaaggcat atgacttatg tgtgtcatca acaaaatcta
     8941 tgactggtca cttattagga gcatcaggtg ctatagaggc tgttgtttgt gctatgagta
     9001 tagaagatag ttttgtgcct ccaactataa acatacaaga agttggtgaa gatttggatt
     9061 tagattatgt tccaaatcaa ggtaaagaaa aaaatattag atatgcacta tcaaattctt
     9121 taggatttgg tgggcataat gctacaatag tactaaaaaa atatgtataa ataaatatta
     9181 aatataaaac taaaaaacaa gcacttgttt taactgtata aatttatatt agcttaaatt
     9241 taaagtctcc taaattattt taggagactt ttttattaag atttaagtct aggatatgtt
     9301 gtaaatcatt ttgatatatt gtattattaa ttattaagag gttatttata tatgcaagtt
     9361 ttcattctac aatatacaaa aggaggacta aaattttgaa ttttagacta ccaaagaaaa
     9421 ggttacccaa aaagataatt tttatatgta taatacttat tagtattatt ttgtttataa
     9481 agtatgacca aaatggattg aaagaaagag aaattataaa taagggaaaa tatatgtctg
     9541 aaagccttgt ggcagataag gagtatgatg aagcaaaaaa agtggttaag acttcttttg
     9601 aatctattcc agaaaaaaaa tacaataaag tatataatga agtttgggat atgttaaaga
     9661 ctatttcata tgttccagat ggaacgaaga tagtcattga ttctttagaa aagatgagag
     9721 aatcagatgt gaaattatct gatgaatgta gatttaatat agaaaaaaga ttagcatcag
     9781 tgtatataat ggataataat tattctaagg cagtagattt aactgtaaag tctataaagt
     9841 tggctgagaa attggggaat aattatgaaa aagcaagact agatgttgat ttggcgagca
     9901 tatttttaaa gataggtggc tatgaaacag gagagaaatt aataaaggat tcttttaaaa
     9961 tagatattga tgaaggtgaa aaaaatggaa tggttagatt atatgctatt ataaatctag
    10021 cagaaatata tgtagaagag ggagaatatg ataaagctat tgaaataagt agcaggataa
    10081 aagattataa aaagtttgta aatacggata attacaatga ttttgatata atggcctcta
    10141 taatgcagtc aaatgcgtat gtgggcaaaa ataatataca aaaggcaaag acttttttag
    10201 aaaaagctga ttatcttata aaatcagata gaacagttta tatattagat aaagatatgt
    10261 actattatat ggcaatggga aacttagaat ataaaagcga aaattataat tctgcaatag
    10321 ataattataa aaaaagttta gagatatcta ccaaaagaaa gttaacccag gagaaaataa
    10381 aaaatttaaa taagatatta aatatatatg aaaaacaagg aaatatagaa tctttaaatg
    10441 agtatcaaaa gatgttaata aatgaatata aagagaacaa aagtataaga gattcagaga
    10501 ctagtttcta tataattgat aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa
    10561 aagaaataaa atattataaa atattattta ccgtaatttt aattgctata ttagggtcta
    10621 cttttttata caaccgattg aagtatttta aagcacaaaa tttacatgat ggtcttacaa
    10681 atgtttataa tagaaaaagt tttgatatta tgtatgaaaa gtacagagag aaagatgata
    10741 attttgcact tgtgatgata gatattgata atttcaagct cataaatgat aattatggac
    10801 ataaatttgg tgacactgtt ataaagggaa taacaaatac catttgtgtt atgttagaac
    10861 aagatgataa agtctttagg tacggaggag aagaattttc tgtattaata agaaataaat
    10921 ctggtgagga agtagcagat ataattgaca gtataagggt tgcaattgca aataaaaagt
    10981 ggaatgatga tgtgactgtg actataagtg caggtgtagc tcatatttca gatagcaaag
    11041 atgtactaga agaagctgat aaaaatttat ataaagcaaa acaacttggt agaaataaag
    11101 tagtatataa gtaattatat actatataaa taagaggtat aaatatgaaa caatcaatag
    11161 ctatagtaac tgataaaata actaaactat catctttctt agaagaaaat atacgtttgg
    11221 ttttaggtaa ttatattgat ataaaccact attatctgga agttttaaaa gagaatgata
    11281 aaatacaagg tgattttgta ctagtaatga atgacgatag attaaacagt atgagaaagc
    11341 atttagtaca agatagtaag attatagtga taaggagaac acttaaagaa aatgaaatat
    11401 atggattatt ttcaatacca aatggaactg aagtattggt tgtaaatgat acaaaagaaa
    11461 ctaccttaga aacgataagc ttattttaca aaataggagt aaaaaattta aagtttactc
    11521 catatattga aggaaaaatt tataaaaata tagacatagc tataacgcct ggagtaaagg
    11581 gaaaagtacc aagttatata aataaagtaa tcgaccttgg aaatagatat attgatatat
    11641 ctacctttat agaaataata aatggtctta gaatagatgt taaagaaata cgtaaaaatt
    11701 taatggcata ttctgagagt ttaataagtt tagataatgg agttaaagat aattataggc
    11761 aactgttttt aaaaatagaa gaacaagatg ttatattgaa tctatcaaaa gatggaataa
    11821 tatttacttc agcagatggt ataataaaca catttaataa agaagcttta aaaattttag
    11881 gtataaatga aaatccacaa ggcaaaaaca tagaagatat catttctgaa gatttaaata
    11941 ttttacttga agacaaagaa ataattgatg aagtggtatt tacaaataaa aaatatataa
    12001 atgtaaataa aaaaaatatt ttttctatgg ggaataaagc tgggatatat tataacttac
    12061 aagaaataac gtatataaaa aaattagaac aaaatcttac taataaatta agagaacaag
    12121 gtcagatagc aagatataca tttaaagata taaaaactaa aaataaaaat atggtaaagt
    12181 gtgtagaatt ggctaaaaaa atatctaaat cagacctttc tgtattaata ataggagaaa
    12241 gtggtactgg taaagaatta ttatcacaat caattcataa tgcttccaat agaagtaatc
    12301 aaccgtttat agcagttaat tgtgcagcag ttccagatag tctattagaa agtcaactgt
    12361 ttggatatga aaaaggctct tttacaggag ctttaagaga aggaaaaaag ggtctatttg
    12421 aacttgctaa taatggaact atatttctgg atgaaattgg agatatgcct ccattgctac
    12481 agacaaagtt actaagggta ttacaagaaa agcaggttat gcctataggt tcccataatg
    12541 ttataaacat agatgttagg gtcatagcag caacaaacaa aaacttacta aagatgatta
    12601 gagaaggaaa atttagagag gacctgtatt atagactaaa tgtattgcca atagatgtac
    12661 catcattgag gaatagaaaa gaagatataa taacacttat gaatttcttt ttgaaaaaca
    12721 atataagact ttcgccagaa gttataacta ttttagaaaa atataattgg ccaggaaata
    12781 taagagagtt acaaaatgtt gcttcttatg taagtctaat gtgtgatagt atggttttta
    12841 gagatgactt accaccatat ataagggtag aatgtgctaa caattatgaa gatggatttg
    12901 gttatacaag aaaagaattt gaggaaatac tgaggattct atatgaaaga agagagtcat
    12961 cctctggaat tggaagaggg tttataatcg aaaagcttat acaggaaggt attgagattt
    13021 ctgaaagcaa agttagaaag gtgctatctt atttaagtga atgtggatac atatcatcta
    13081 aatctggtag aggaggaagt caaataacct cagaggggat aaaactttat aataatataa
    13141 attaagctaa gtatttgata ttgaaatcac atattttgaa tttagatatt taaataataa
    13201 taaactttta tatttataga gattaattta tattatgtca gaaagaatat tgtaatttac
    13261 acttaaatat taataggttt ttaaaatagg attagatagg taataggtta agttagaata
    13321 acccattacc tatttttatt tgtaagaaaa gtgtacatag aattagaagt aaaaaataca
    13381 actaataaaa ctattgaaaa tactagataa ataataaata aaaataatta tagattttgg
    13441 catacatatt gctaattata gagataagac gacaaaataa ttgggaggtt taatgatgaa
    13501 atacaaaaca acagggactt gtgctacaga aattgaattt gaagtaaaag aaaataaagt
    13561 tacaaatgta aactttatag gtggatgtga tggaaattta aaagggctaa aagtattagt
    13621 tgaaggcatg aatatagaag atgtaataca aaagttaaaa ggaatagagt gtaaaacaaa
    13681 gccaacatct tgcccagacc aactgtctct agcattggaa aattatatga atataaagta
    13741 atacataaaa caatcgatta gttaggagtt taattatgaa accaataata gggattttag
    13801 gaaatttaat aataatggaa aatggtatgt ttccaggtct tgaaaggtca tatgtaaata
    13861 atgattatat aaatgcagtg ctaaaaggtg gcggtagtcc agtaataatt cctgtaaata
    13921 cagataaaga agttataaaa aaacagattg aaatggttga tggagtctta atatctgggg
    13981 gatgggatat caatcctcaa ctgtatggag aagaaacaag agaagaaacg acatttatat
    14041 atccagaagt ggatgaattt gatttaattg caataagtat agcattagaa cttaaaaagc
    14101 ctatattagg tgtatgtagg ggactacaaa ttttaaatgt aagtttagga ggaactttat
    14161 atcaagataa taatcttata gaaggaagtt atataaaaca tacacaatct tctaaaagac
    14221 atgtagctac tcataaagtt gatgttaaag aaggaagtat tttagaggga attttaggaa
    14281 aacaattact tacaaatagt tatcatcatc aaagtgtcaa tcagcttgga aaaggattaa
    14341 aagccatagc atactcaaaa gatggtatga tagaagctat tgagaaagag gatgaaaatt
    14401 ttgtagtagg agtacagtgg catccagaaa tgatggtaga ttactgtgat aaaatggaaa
    14461 aactatttaa atattttata aatatttgtt cgaatttata atataccttg gggggattga
    14521 acatgaacga aaagaacaaa atgggattaa taagtattat acttttaggt attaatgcag
    14581 tagtaggagc tggagttttt ctattaccag gagatgcaat gaaatcattt ggtgtagcaa
    14641 gtatatttgt atatatattt gatatgttgt tagttttatc tatggcattc tgttttgctg
    14701 aggttgcagg taaattcaat aaaaatggtg ctgcctatgt atatacaaaa gaagcttttg
    14761 gagatttttg tggttttgaa gtaggtctta tgaaatgggt aataggatgt atttcttggg
    14821 gagctttaat agtaggtttt cctacatcat tatcagcagt atgggctcca gcaggtgaac
    14881 cacatataca aaaaattata attgtagcca tgatagttgg gcttacaatt ataaacttat
    14941 taggtgtatc tctatcaaaa attgtacaaa atgttataac tgtaggtaaa ctaattccat
    15001 taatactatt tataggaata ggtatatttt ttataaaagg agttaatttt acttcttcaa
    15061 caatggtacc tccaggagct ggagcaacag agtttggagc agcagcactt ttaatgtttt
    15121 attcatttac aggatttgaa tctatagcag ttgcagcaga agatatggag aatccacaaa
    15181 aaaatatacc aatagcaatt ataagtgtaa tagtaattgc atcaataata tatatattaa
    15241 atcaagttgt atgtgttggt atactaggag attcattatc tagtacatct acaccagtgg
    15301 cagatgctgc aagaatatgc tttggaaata tgggagctgg attggttacc tttggaacat
    15361 tggtttctgt tggtggaatt tgtatgtgtg gtgcttttgt aaatccaaga agttgtgtag
    15421 ctttagctga tgataaaatg ttaccgagaa tatttgcaag aaaagataaa aaaggtacac
    15481 catatgtagc gataatagct actatgttaa tcactatacc aatagctcta tctggaagtt
    15541 ttgctgaatt agcagcaata agtgcagttg ctagattcat acaatacata ccaacatctt
    15601 tatccgtatt agtgtttaga aagaaaagac cagaattagt aggtactttt aaaactcctt
    15661 ttggagcagt gattcctcta attgctgtat gtgttggaac atggttatta ttccaagcat
    15721 ctatgcatca attaattatg ggattaggag ctttggcaat tggagtacca ttatacttta
    15781 taatgaagtc ttataataga aaagtctatg gtgaaaattt aaagaaaata gtttaattat
    15841 gttataacag tttattgagt taaagttaga cattggcaga aaattaaata caaataagta
    15901 tctataaggc tatattaaaa tattattaat tttttatttt aatatagcct taaattgtta
    15961 tcaataagtg aaaaatattg taacaataga agaacaatag tattatgttt atagacaaaa
    16021 aacttattag gaggtaaatt tatgccaaca ataacattaa aaaatggtgt agacgtactt
    16081 attagagaag gagtaaggga agatgctcaa agcattatag atttttataa tgaagtgggt
    16141 ggagaaactc attttctgtc atttggaaag gatgaatata aaatatcttt ggaggaacaa
    16201 gaaaatgcta tagaatcagc aaaagcatct gataattctg ttaaactgat tgcatttata
    16261 gatggtgaaa tagttgggat tgcaacgatt gattctaatc aaaaagcaaa aggaaagcat
    16321 gttggagtat taggaattgt tgtaaaagaa aaatattggg gaattggatt gggtaaaagg
    16381 cttatgttag accttataga atggtgtaaa tcaaatggaa taaccaagaa gataactctt
    16441 gtcactaatg aagaaaatta taatgctata ggattataca aaaaagttgg atttgaagta
    16501 gaaagcatac taaagaaaga atgttattat aatggtgttt atactgattt aataggaatg
    16561 tcacttttat tgggaatata attagtgtac attttatctc gaaaaaagta tattaagtat
    16621 ggtataatta aaaaagaata taacttaata taattttgga ggacgaatta tgaaaaatct
    16681 gtgtaaaata tcagatgatt atttaaacag caagcttaag tatttaaagt tattatcaaa
    16741 acaatatcca agtatatcaa aagcaagtac agaaataata aatttagagg ctatattaaa
    16801 tcttccaaag ggaacagagc attttataac agatgtacat ggtgaatatg aaccatttgt
    16861 acatgtatta aaaaatggtt ctggtgtaat aaagagaaaa atagaagaat tgttttcaaa
    16921 tacaataaga gacagcgaaa aaaaaatgtt agcaactctt gtatattatc ctgaacaaaa
    16981 gttagaccta ataattaagc aagaggaaaa tatagatgat ttttatagaa taaatattta
    17041 tagacttata gaactttgta aatatgcttc tagcaaatat actaggtcta aagttagaaa
    17101 actgttacca gaaaatttta aatacataat agaagaactt ttgcatgagc atgttaaaag
    17161 tgaacataaa gaagagtact ataaaagtat agttgaaaca atagttgaca taggaatagc
    17221 taaggagttt ataattgcta tttctacagt tatacaaaaa ttggttgtag atagacttca
    17281 tgtaatagga gatatttatg atagaggtcc aagaccagat ataattgtcg ataagcttat
    17341 tgaacatcat tgtgtagata ttcaatgggg aaatcatgat atattgtgga tgggtgcagc
    17401 ctcaggtgaa aaaacttgta tagcaaatgc actgaggata tcagctagat atgcgaattt
    17461 agacattgta gaagacatat atggaataaa tctgttgcct ttggccactt ttgctataga
    17521 aatgtataaa gatgaccctt gtaaagaatt tattccaaag gttaatgacc aaagtgtaac
    17581 aacaacagaa aagtctttaa tggcaaaaat gcacaaagca ataagtataa ttcaatttaa
    17641 gcttgaaggt gaggttataa gacgaagacc tgaatttgaa atggaacata gactcttgct
    17701 taatatgata aactatgatg aaggtacgat taatttaaaa ggtaaaacat ataaattaaa
    17761 agatacttat ttacctacaa tagacaaaaa agacccatat aaattaacta tggaagaaag
    17821 aaatgttatt gataaattag tatcttcatt tagaggtagt gaaaaattac aaaaacatgt
    17881 ttcatttttg ttttctaaag gaagtatata tttaaaggct aactcaaatt tactgattca
    17941 tggatgtgta ccgttaaatg aagatggaag ctttatgtct atgaatataa tgggaaaaga
    18001 gtataaaggt aaggctctta tggataagat ggagtctcta gcaagagaag gatttttctt
    18061 taaagataag gcagaagaaa aactgtatgg catggatatt atgtggtatt tatggactgg
    18121 gaaatgttca tcattatttg gtaaagatga tatgactaca tttgaaagat actttatagc
    18181 tgaaaaagag actcataaag aaaataagaa cccatatttt aaacttagag aaaatgagat
    18241 ggcttgtaaa agattatttg aagaatttga tttagaactt gatgaatctc atataataaa
    18301 tggtcatgtt cctgtggaga gtaaaaatgg agagagccca ataaaagcta atggtaaaat
    18361 tctcgttata gatggtggtt tttctagggc ttaccaaaaa actactggta tagctggata
    18421 cactttgata tacaattcta gaactttgca attagtatcg catgaaccat ttaattctgc
    18481 tgaagaagcc attgcaaatg aaagtgatat cttatcgaca actgttgtag tagagcataa
    18541 ggcaaagaga aaaatggtga gagatactga tgaaggagta aagatacaag aggaaataga
    18601 agatttaaaa ctacttttaa tggcgtataa gaaagggcta ataaaagaaa tgtagatgtt
    18661 atagaggcac ttcatgattg aagtgtcttt ttgttaatac ttattaaata tattataaag
    18721 attaataata gtagtttata ttatacttac taagtgaatc caagataaat gggttcactt
    18781 tttttattta taaacattat taaaaaaata tagatgtttt ggttatattc cttgtccttt
    18841 ataaatagaa ttatataaac aaaggaggag gacaagagat gaataaactt tctgatgaaa
    18901 taataaactc tctttctaca actataagag aaaaggtaga aaaagtctca aacaataatc
    18961 tgaacataga agaaattagg ttacgctcac aaaaaccatt gatactaaat gcaaactcta
    19021 aagattattt ttacaatcaa aaaactatga cacttgattt aaatcaacaa aactcatatg
    19081 tagtaacaag agaagatgta gagcaaactt ttcaaataat atgtaaatat tcaatacatt
    19141 catttatgga tgacataaaa aaaggattca taactttgag aggaggtcat agagtagggt
    19201 tagttggaaa agcaatagta gaagatggac aagtcaaaaa tataaagcat atatcatctt
    19261 taaatataag ggtatcaagg gaaattattg gatgctcgga taagatttta agtcatatca
    19321 taaagggaaa aaatcagatt aataatactt tgataatatc accacctcaa tgtggaaaaa
    19381 caactttaat aagagatata gtaagaaatt taagtaatgg taatgaagat tatggattta
    19441 aaggtttaaa agtggcttta gtagatgagc gtaatgagat agcaggatct tatctaggtg
    19501 ttccacaaat ggatgtggga ataaggactg atataataga aacttgccca aaagacttgg
    19561 gtattacaat gcttttaagg tctatgtcac caaatgttat tgtgacagat gaaattggaa
    19621 gtgaaaaaga aataaaggca ttatacacag cattaaatgg aggtatagga ttaataacta
    19681 ctgtgcatgg agattcaata gaggatatac aaaatagaaa agaattaaat agacttctgg
    19741 ataaagagtt atttaaaaag gttataattc tttctgcaaa aagaggagca ggaacaattg
    19801 aaaaaattta tgatttagaa gaaaagagat ggtattttgc aaattaaaat aattattata
    19861 gctttcttga taggaagtag ttatttgata ggtgaacaaa tatacaaaac ttatactcgt
    19921 agacataaac aattaaacga tttaataaga gttttagaaa ttttgaggat ggatttatct
    19981 tttggacttt atacattaga agaaatcttt aataggatag gtggaaataa agagttttgt
    20041 ttttggaaat ttttctatca aatatcagaa ggacttcaca atgaacaaag taaaactttg
    20101 gaaataataa tatcagaaaa tattgatgta ttaagtaaag aaacttatct aggcaataaa
    20161 gaaattgagg aactgaaaaa tctaatactt actttaggga aaagcgatat agagtcacaa
    20221 caaagaatga tagatttatc tatagaaaat ttgaaaaaac aaacctatga gacaaaggaa
    20281 gatataaaca aaaaaggcgt attgtataaa aaattagtga cattcatagg tataggtata
    20341 tgtattatat tgatttaata tggggaggtt tattatggaa atatcattga tattaaaggt
    20401 tgcaggtgtc ggtattctaa tatcagtatt gaatatgata ttggaaaaaa cagatagaaa
    20461 agattgggca ggtcttacta cattagcagg tgtaataatc gtattgggta tggtaataac
    20521 agaaataagt gacttgttta atactgtaag aacaatgttt caactttact aaagaggagg
    20581 gagtaatttg gaaataatgc aattaatagg aattgcaatc atttcaacta cactctgttt
    20641 agtaattaaa aaagatagac cagagatagc aaatttcata gctataataa ctggagtaat
    20701 aatactattg tcagttatgt ttaagctaaa ttttatagta gatagtatac aagatttggc
    20761 taacaaggct aacatcccga ctatgtatat atcattaata ataaaattaa ttgggatagc
    20821 atacttaatg gaatttgcga tacaactttg taaagattgt ggggaaggaa acattgcttc
    20881 aaaactagaa tttggaggta aaataatagt tatgtcaatg tctttcccta tacttttgtc
    20941 cattgtagaa atggtggtga atataattcc atagatgtgt atcttggaat gaatataagt
    21001 atattagaga gctttcgttg tattgaggtt tatagagtga agagaggcat ggttgcttat
    21061 gaaaaaaaga tttttaccaa tgattatagg ttttttattt actttctttt ttgtaaatat
    21121 ctttgctatt atgatatttg ctaatgaagt accaagtagt gaagataaag aatatggtga
    21181 aacaaaaaat agcatagata agtatataga tggtcaatta gacaagctag atataaatga
    21241 gattcaagac tatataaata aagaaatagt tattaatgat gttaatttaa aatcatttgt
    21301 aaaagattta attagtggag aaaaaaatat cttagattta ttcaataaag atggattgaa
    21361 gatattgatg tttgatgaat ttaaagcaag tttaaaggta gtagcagtaa ttttggtact
    21421 ggctctgcta tcatctattt taaagagtct ggaaaactct ttttcatcag gtgcagtaag
    21481 tcaaatagca acatatataa tatttatcac aatggtatca cttacactta ttggttttaa
    21541 agatgtatta cagatttgct atgatgcaat agaccatact gtaggtctta tgcaagttat
    21601 aatgccaata ttaataacat ttttactttt aattggattc ccaataacgt cgactacttt
    21661 gaatcctatc ttcataggag gagtaacatt tataaatgta ttttttaaaa attttctatt
    21721 tgtatctatt actgttgcat ttggaatttt aataataaac aatttatcaa aaaatataag
    21781 gctaaagaga tttttctcct ttgtaaaaca aataaattat gtttctattg gagctatgtt
    21841 tactgtttac ttaggtcttg tatccataca aggactgtat gtaaccagtt ttgataaatt
    21901 cagtgtgaag actgctaaat ttgctatagg taattttatt cctgttgtag gtggatttgt
    21961 atctgactct gtggatattt tattatcttc atctcagctt ataaaaaata tatttggagg
    22021 aatagggctt atcttacttg ttggaatttg cttacttcca gtaataaaaa ttttatcagt
    22081 aatagtagtg tacaaattag cagcaataat tgtagaacct gttggagagg atggaatatc
    22141 taatttttta aatgaagtgg caaatttaat gataataatg ctagcatcag taattgctat
    22201 aacagtaatg ttctttgtaa ctgtagctat cttgacctcg ataagtgtcg taagtcaagg
    22261 gtaatttata tatcagtgaa aaataattac atttaatcta atatttttag agaggaggat
    22321 gctatgttag agggcataaa ggcatggata gttagtgttt tgataggagc atttatagta
    22381 aatatagtag atatgatttt accaagttct aagataaaac cttatgtcaa cctagtttta
    22441 aactttatgt ttgtatttat tgtaataact ccagtagtag gatttttttc aaaagatatg
    22501 agtttagaag atagaatatt aaaatctatg ggaaactaca acaagcagta tgtagatagt
    22561 acaaatgctt tggcaaaaga gactggaaat aatagtctat ccaagggata tgaggatggg
    22621 ttaaaagaag tacttaaatt aaaacttgat gaatatggtt atgatttaga agatatagag
    22681 cttaatgggg caaatataaa taatataaaa ataaaagaaa agaataatag tgcaaaaagt
    22741 aataacagtt ccaatgaaga aaataaaaat aatagtacta aaagagagga agaaaacata
    22801 aactctaatg acaaagaaaa ttctaaacaa gtcttcaaaa aaggtacaga gtatgggttg
    22861 aacctaaatg aagaaaaatt aaaaaacgac ttaataaagg tgctggatgt ttctatagaa
    22921 gatatacaaa ttgataaata ggagttggga gaatgttgaa aaacttaaat gacaaagata
    22981 agagaaaggt atattcacta ataactatag caggaatatg tgtggtttca ttagtattat
    23041 tatcttgttt tcctagtagc aaaagcgata aacaagttgg aaaaacagag actaataaaa
    23101 atgctgaaaa gcaagtcact aaagaacaag aaaaagatga tttagagtca aaattaacag
    23161 caatactatc taaaattgat ggtgcaggag acgttgatgt aatggttaca tttgaatcaa
    23221 gtgaagaaat acaacctgct tttaattcaa ataatacaac agaaacaaca gaggaaaaag
    23281 atgcacaagg tggagaaaga acagttacga cttctagtga aaataaaaca atgataactt
    23341 caaattcaag tgaccctgta gttataaaaa ctacagagcc aaagataaag ggagtgatag
    23401 tggtagcaag tggagcaagt gacccaagtg ttaaagagac actttatagt gcagtacaaa
    23461 catcattaca agtagctggt catcaagtag aaatatattc taaataaata catataaccg
    23521 gggggataaa tgaatgaagt ttaattataa gggaagagga tttgtaataa taactttaac
    23581 tgcaatgtta gtggtagtag gaacagtgaa ttatcaatta agtaagaaat ctttattaga
    23641 aacatcaaaa gagtttaagg catatgaaga agcacagtta caaaaaaata ctgatgagag
    23701 tgatagtagt aagacagagg attctagtaa tatagataaa caagatggta aggaaagtgc
    23761 tgatattgat atagttgata gcaaagctag taaagtaaaa gaaaaggcaa ctgagacaag
    23821 taaggaaata aaagctcaat tatcatcaga gaaaaatatg aaaaaggcat cttatatttt
    23881 agatatgaaa atgaatagag aaaaacaaag aaatgagtta gttcaagatt taaatgaaat
    23941 gataaataat ccatctacaa cagaagaatc tagaaaagaa gcttctaata tgaaattaaa
    24001 tatagttaaa attcaagaaa aagaacttca aatagaaaac cttttgagta caaaagggta
    24061 tgaagaagcg ttagtttata taagcgataa caaagttaat gtagttgtaa atgaagctaa
    24121 attagaaaag aaagatgcag caaaaatatt tgatttagtt gcagaacaag ccaatgtgaa
    24181 gtatgaaaat attaaactta caaataataa tagtaataag taaagtataa acctcagaat
    24241 taactgaggt tattttcttg caaagtagac ttgtatttgg tattattata cttataaaaa
    24301 tagttcctta aaatcaggag gtaagtttta atatggaaga taataaattt ggacaagtaa
    24361 aaatatctaa tgatgtaata gctacaatag ctggtttagc agcattagaa gttgaaggca
    24421 tagaaactac agcaacatta acagataagt tgttaaaaaa taatggagta aaaatacaaa
    24481 tcgaagaaga ggatgttaat ttagatgtaa tggttacgat aaagtatgga atgtcaatac
    24541 cagatacagc ttttaaagtt caagaaaatg taaaaaatac tgttgagaca atgacaggac
    24601 tgaaggtttc tcaagtaaat atacacattc aaggaatcag ctttaagaag gataaagttg
    24661 ataaagaaga agcaaaagca actaagaaaa attaatatta gccctctgaa aagtcagagg
    24721 gttttttgct ggtaattgat tttatatgga taattaaact taaaggagaa ttttgattga
    24781 tgaaaaaaga tagggcacaa aagagtacaa caagagaata tataatgaaa cttatatacc
    24841 aaataaatat taataaagaa gattttgaaa ctttagaaga taaagtagat aattttttaa
    24901 aagataattc agaacatatt ataaatagat ataaagaact tgcacttcaa tactcaaaaa
    24961 atacaaattt aaagttagaa gatactgaaa ttgaagatgt tatagacaaa aaatatataa
    25021 atacagtatg caaagcattg aaagaaaatc atgacaaaat agatgagtta ataaataagc
    25081 atgctaaaaa ctggacagta gatagaatgc caaaggtaga tgtttctata cttagattgt
    25141 ctgtgtgtga aatactttat ctagacactc ctaataaagt atcaataaat gaagcagtag
    25201 aacttgcaaa aatatattgt gatgataagt ctcctaaatt tataaatggt atattgggaa
    25261 gtgttgttga tgaaattggg aaataatata ataattggaa ttgatactag ctgctatact
    25321 acttctatag cagctatctc tttagataag aaagttatat taaatgaaaa aataatgtta
    25381 gaagtaaggg ataattcaaa aggtttaagg caaagtgaag ctgtatttca acatatcaat
    25441 aatttaggga ttcttagtga taggataaaa tcttttaaag ataagtttaa tgtagaagga
    25501 gtttgctcat caaaaaaacc gagacctgtt gaaaactcat atatgcctgt attcaatgta
    25561 ggtcataatt ttggaaaatt attgtctagt atatatggtt gtaggtttta tgagactact
    25621 catcaagaga atcatattga agcaagttta ttaaatagta aattaaaaaa taataataaa
    25681 tttatttctg ttcatatgtc tggtggtact acagagatac ttttaacaag caaacaagat
    25741 agtcatcaca atgtatgtga tactaattta ggtaaaattg ccaaaataag tattaaaaaa
    25801 gatgataaaa gtaagttata caataatttt ggatataata tagatataat tggaggaagt
    25861 aaagatatta gctttggtca acttatagat agagtaggaa ttaagcttgg atataaattt
    25921 ccttcaggaa agtatttgga tgaaaatgct ttaaattgta atcttaaaat agaaagtggt
    25981 ctgaaaactt ctgtaagaga tgggtatatg aatttatcag gattagaaaa tcaggtaaat
    26041 aagattataa atgataatgg agataatact aatcaaaaag aatatatatc taaattggta
    26101 ttagattcag tagttagaaa tatgtttaaa tctttagtgt atttatgtga aacttataat
    26161 gtaaatgaag tagtatttgc tggtggagtt tctgcaagta agtatattct tagagaatta
    26221 agcatgaagt taagaaaaaa gcatatagaa gcttatttta cagaaccaca atattcaact
    26281 gataatgctg tcgggtgtgc tataatagga ttgaataact ttttaggaga aagagtatga
    26341 aactaagagc tttagatata agtgaagcaa actcatatat taaaagaatt ttaataaacg
    26401 acccaatact ttctaatctt aaagttaaag gagaaatttc taattttaag gttcatagta
    26461 gtgggaatgt atatttatca cttaaagatg aaacatcaaa gttaaactgt gtaattttta
    26521 aaagtaattt taatcgcaat ttaaaattag ataatggtgt aaaaattata gctaatggat
    26581 atatatcagt ctatgaaaga gatggtgcgt atcaattata cattaatgaa attgaaatag
    26641 agggaatagg aaatttacac atagagttta atagattaaa ggaaaaactt aataaagaag
    26701 gattatttga ccctaaatac aagataccta tacctaaaat gccaaactca attggcgtta
    26761 taacatctcc aacaggtgct gttataagag atataataaa tgtaataaaa agaagatatc
    26821 cgaaagttaa tataaagctt tatccagtaa cggttcaagg agataaatca gctgaagaaa
    26881 tctgtgaagc aattagattt ttcaatcata tgaagaatgt tgatacactt atagttggta
    26941 gaggtggggg ttcaatagaa gaactttggt cttttaatga tgaaatggta gcaagagagg
    27001 tttttaattc tcaaattccg ataatatcag cagttgggca tgaaacggat tttactattt
    27061 gtgactttgt gtctgatatg agagccccaa cgccatcagc agcagctgag attgctactc
    27121 catcattgga tgatataaac tataaacttg gaaacataaa aagtaggatg agtaagtctc
    27181 ttacaaatca aatagagcta gaccaatata ggttagaaac agtatttaat aagataaata
    27241 actatttaga ttcgtacaca ataaaagata aagttataca attagacaaa atatatgata
    27301 aaataatttt tggaatagaa aataatttaa aattagaaga tgaaaaatta gtaaaaattg
    27361 gagcattact ccataattta agtccattag ctactatgga tagaggttat agtataactc
    27421 aaaaaaatgg aaaagtaata aatagtataa aaggattaaa agtaaaggat agtatagata
    27481 tagtattaaa agatggaaat ttagaatgta tgatagataa aattgaaaat aaagagggat
    27541 agtatgaatt taacctacga agaagcttat aaaagattag aaagtatttt aagtgaatta
    27601 gaatctaaaa atgctagctt ggatgaatct ttaagcctat atgaagaagg tataagcctt
    27661 tataaacatt gtaataagct tttagatgat gctaaattga aaataagtaa atttaatcaa
    27721 ttgggaatag aagaagactt taaaatagag gaggaataaa attggaattt aaacaatgtc
    27781 taaaagaaaa agcaagtttt gtagaaaaag tacttaaaga atatatgcct aaagaagaag
    27841 ggtatcaaaa aactgttatt gaagctatga actatagctt aagtgctgga ggtaaaagat
    27901 taagaccaat acttacattg gaggcttgta aaatagttgg aggtaatgaa gatgaagcta
    27961 ttccttttgc tattgcaatt gaaatgatac atacatattc actaatacat gatgatttgc
    28021 cagctcttga caatgatgat ttaagaagag gaagaccaac aaatcataaa gtatatggag
    28081 aagctatggg gattttagca ggagatgctt tgttaaacta tgcttttgaa gtaatgcttg
    28141 caggctcaat caataaagaa aatccagaaa agtatctaaa agctataaat gaaattgcca
    28201 aaggtgctgg tatatatgga atgataggtg gtcaagtagt agatgtagaa agcgaaaata
    28261 aacaaattga aaaagaaaaa ttagattata tacatatgaa taaaactgct gcaatgatgg
    28321 taggctgtat gagagcagga gctactatag gtggagcaaa ttctgaacaa atggaagaaa
    28381 taactaaata tgcaaaaaat atcggattat cattccagat agtagatgat atactagaca
    28441 tagtaggaga tgaagctaag ttaggtaaaa aggttggaag tgatatagaa aatcataaat
    28501 caacataccc atctctttta ggtttagata aatctaaaga aattgcacat aatttgattg
    28561 atgaagcaaa gaaaagtata gaaaaattat ctgatgatgt agatttttta aagggattgg
    28621 ctgaatatat aatagataga gaatactaaa atatacagct aaaatcgagg agggaaaaat
    28681 ggattttttt tcggagattt ttaacaatgg ggctttggga ataagtctga ttgcttgttt
    28741 cttagcacaa tttataaaaa tatttactgg taaggaaaaa agaattgagt tatcaagaat
    28801 acttatttca ggaggtatgc ctagttccca tagttctttt gttacaagtt tagctacagt
    28861 ggtaggtatt gaaaaagggt ttaattctac tgattttgct ataattaccg tgcttgcctt
    28921 aattataatg tatgatgcag caggtgttag acgggctgtt ggtaaacaag ctactatatt
    28981 aaatcaaatg gtggcagata tacagcatgg aaagcatata gagcagaaga aattaaaaga
    29041 attaataggt catacaccac tagaagtttg gtttggagct ttacttggaa tagtaacagc
    29101 tttaatattg atgtaaattg aaaatttaca gaataaaggt gaggataata tgtataaata
    29161 tttagataaa gtaaattctc caaaggatat aaaaaatatg agtatagaag aaatggattt
    29221 acttgcaaag gatataagaa agttcttagt aaaatctgtt tcaaagacag gaggacactt
    29281 agcttctaat ctaggagtgg ttgagttaac attagcactt cataaagtat ttgatagccc
    29341 taaagataag attgtatggg atgtagggca tcaatcttat gttcataaaa tagttactgg
    29401 aagaaaagat tgttttgtat ctttgagaca gtttaatgga ctaagtggtt ttccaaagga
    29461 gaatgaaagt cctcatgata tttttgatac aggacatagc agtacatcta tttctatagc
    29521 tacaggtatt gcctgtgcta gggatataaa aaaagaaaac tatagtgtta tatctgtgat
    29581 aggagatggt tctataacag gaggtatggc acttgaagct ttaaatcaat taggatatat
    29641 agataccaat atgatagtaa tccttaatga taatgagatg tccatagata aaaatgttgg
    29701 aggaatgtct aaatatctgt caagtattat aagaaactca acagttgaga aaatgacaga
    29761 tgaagttgat aaaattttaa atgttacaca aacaggtgaa attttatcta agactgcaca
    29821 tagatttaaa gataaactaa tgtatagctt ttctcctcag gactgttcat tttttgattc
    29881 attaggtata agatattatg gacctataga tggccataat actaaagaac taatagatat
    29941 attgagaaaa gctaaacaca aaaaagggcc tgttctttta catgtaatta cgaaaaaagg
    30001 aaaagggtac agatttgcag aagaacaacc tgataaatat catggagtat caaagtttga
    30061 tataaagaca ggtgttacat ctgcaaaagt taagtctatg tcaattagtg ttggagaaaa
    30121 acttgttgat atggctaata gtaatgaaaa tatagtagca ataacagctg caatgccatc
    30181 tggtacaggg ctaaacctgt ttgaaagtgc atatccaaaa agatattatg atgttggtat
    30241 agctgaacaa catgcaacag gatttgcagc aggtcttgca aaaaatggta tgaaacctta
    30301 ctttgctgtg tactcatcat ttttgcaaag ggcatatgac caagttattc atgatgtatg
    30361 cataacaaaa aagccagtta cttttcttat agatagagct ggattagttg gaaatgatgg
    30421 agaaactcat catggtatgt ttgatttaag ttacttaaat tctattccaa atattgtagt
    30481 aatggctcca aaagacacaa gagagatgga gcttatgatg gatttgtcat taaaattaga
    30541 ttgtccacta gcaattagat acccaagagg aagtagttat tacctagata agggagagta
    30601 tggagagatt gtactgggta aatatgaggt attagatgac ggtcaagata cagtcatact
    30661 ttgtattgga agtatggtaa aacatgcttt agaggctaaa gaaatattat caagagaagg
    30721 aataaatccg acaatagtta atgctagatt cttaaagcca attgatgaag gtatgttaaa
    30781 agcattatta aaaaatcata aaaatgtagt tactatagag gataatattg taactggagg
    30841 atttggaagt agaataaata agtttattat agataatgaa tataacgtaa atatacttaa
    30901 tatagcaata ccagaagaat ttgtaaaaca tggcaatata gatgaattat atgattttgt
    30961 aggattatcg ccaaaaagta ttgctgataa gataaggaaa ttagttattg agtaaaagtc
    31021 taatgtagat attgtaaatt aaaatttata taaaatatag aatttatcaa ttagaataaa
    31081 tatcaagtgt gttacttcaa gctactttag ttatcattaa agtagctttt aacagaaagg
    31141 taattgtatg aaaaaaagaa tagatttatt attagtagaa caagggtact ttgaaagtag
    31201 agaaagagcc aaaaaagcaa taatggctgg attggttttt gttgataatc aaaggtgtga
    31261 taaggctggt acagaagtaa aagaagattg tagtatagaa gtaaaaggaa atccaatacc
    31321 atatgtcagt agaggtggtt taaaacttga aaaagcaatg aaaaactttg atttgactat
    31381 tgatggaaag gtatgtatgg atataggggc atccacaggt ggttttacag attgtatgct
    31441 caaaaatgga gcaataaaag tattttctat agatgttggt tatggtcaac ttgcatggaa
    31501 attgagacaa gatgataggg ttgtgtgtat ggaaagaacc aatataagaa atgtgacaat
    31561 agaagataca aagcagtttg ctgattttgc gtcaattgat gtgtctttta tatcgttaaa
    31621 gttagtactt ccaaaggcaa aggagttagt acgtcatgat ggggaagttg tcgctttaat
    31681 taaacctcaa tttgaagcag gaagagaaaa agtaggtaaa aaaggagtag ttagagaaaa
    31741 atctacacat atagaagtta taaaaatgat ttctgatttc tcagtcgaaa atggatttga
    31801 gattttaggg ttagattttt ctcctattaa aggtcctgaa ggtaatatag aatatctaat
    31861 tcatttgaga aatggaaatg aaggatatga atttgatggt gagacataca ataacaaaat
    31921 agtagaagtt gttgaagctt ctcataattt agacaaatag gttaaaaggg ggcttagatt
    31981 tgatcctcga attatatatg aaaaattgtg ccttagttga agaactaagg ttgaatattg
    32041 ataaaaactt aaatatactt acaggtgaaa ctggttcagg aaaatctata ataatagatg
    32101 cacttggact ttgtttaggt gataaatatg ataggtcttt tttaagaaaa ggaacagata
    32161 agggacttgt tgaggctgta tttttttcag acaatagata tcttaaaaaa atattagaag
    32221 aaaatgatat aagtatggaa gatgataatt tattggtaat aactaggttg atatactcag
    32281 atggtaagag tactgctaga gtaaatggaa ggactgttaa agtgtctctt cttaaagaaa
    32341 ttgcatctac acttatagat atacatggtc aacatcaaaa tcaagctttg tttaataaag
    32401 atactcattt aaaatttcta gacctttttg gagaaaatga attggaagag tttaaaattg
    32461 cttataaaaa ggtgtaccat aagtatagtg aagtaaaaaa agctttaaat tgcttgacag
    32521 aaaataaaga tgagatgcaa attcaacgag aaatagattt attaagattt caaataaatg
    32581 aaattgaagc tgcaaatcta aataagaatg aatatgaaga tttattaaaa caaagagaag
    32641 tttatagaaa tagtgaaaag atatataata atctaaattc gagttatagc aagttacata
    32701 atggtgagta taatgttatt gacctaatag gtcttgcatc taaggaactt aatgacatat
    32761 ctaaatatga cagtgtttta agtgaatata gtgatactgt agaaagaatc atgtatgagt
    32821 tacaagacat atctggtgag ataagaaatt acaaagataa tatagacttt gaaccttatg
    32881 agctagagca gattgagctt agaatcgacg agattaataa cttaagaaga aaatatggag
    32941 acagtataga tgatatattt gagtattatg gaaaaattaa agatagatta gatgaaatat
    33001 taaacagaga tgaaagagtt gaacagttaa gaagcaaact gatgaatata gaagaagatt
    33061 taaaaataaa agccagtaaa ttgactaaag caagaataga agtagcaact actttagaaa
    33121 aaatacttct tgatgaatta aaaagcttaa atatgaaaaa tgtaatgttc aaggttaatt
    33181 ttgaaaaagg tccatttact ttagatggag tagatgatat tgaatttatg atatctttta
    33241 acttaggaga agatattaag cctatatata aagttgcatc aggtggtgag atgtccagat
    33301 ttatgttagc gtttaaaacc atattggctg atatagatga tattgataca ttagtgtttg
    33361 acgagataga tactggaata agtgggatag cagcacagat agttggtgag aaattgagtg
    33421 atatagcaaa gaaaaaacag ataatatgta taactcattt acctcaaatt gctgcaaatg
    33481 cagacactca ttattgtata gagaaagata cttcaaataa taggacgttt accaatgtta
    33541 gtaagttaaa tgaatctcaa cgaaaaaatg aaattgccag acttatagca ggtaataata
    33601 taacagaaaa aactattgaa catgcaagtg aaatcattga attggctaaa aaatgttagt
    33661 gaaaaacaag cattttaata ggtgtaaaga atttttatag aaatgcttta cacctatttt
    33721 ttataatgaa attattttaa aatatctagt gcctatattg aagaaaagta ggtttgaaaa
    33781 tggaataaaa tttgtataac tttgtaactt tgaaatttct aaatgcttaa gtataatatt
    33841 atttattaag gtagtagtga ggggaggcat aaaatgaata aaaaagatac tatgcactta
    33901 atttttataa ttgtaatttt tattttatta tggtatattg tggtagcagt aagtgagatt
    33961 aataaaaata ttgaaagttg tagtaacaaa ttagattatt tgtcacaaga gcttatgagt
    34021 actaagtcgg atattagtaa tactataaat gaagaaatgt caaaaagtta tataacaaaa
    34081 agtattgact taaaagtaaa aaaaatcgag aaaaaagaat gtattattga tgtagatgtt
    34141 caattaagta aacttggaaa aaatggaaaa gtatttttta tgtataaaga agatagtgat
    34201 aaatggaatg aaactgagat gacaaaatat ggagaattat catatgcatg tgaaataaag
    34261 ataaatacag gaagtgaata tgactataaa gtagttacaa gtggagctat atctgaaagt
    34321 tctgatgtgc aaaaatttac taaatctgat tatatgcctg aacaacccat atttaattct
    34381 gggataagag ataatagaga atactttatt gaaatagtag aaaatagtaa tcttttcaat
    34441 catgagagtg aaaagtcaaa aaataaagct tacgataata ttagattaga aaaagtagat
    34501 attattgtaa atgaaggaaa aaaagacact atatacaaag ccaaattagc tgaaggctta
    34561 gatgatggca aaactaataa ttcaaataga aatcaagtga attatgaagt aagttttcct
    34621 aaaagggata ttgatgacat aatttatatt aaagctaaac ttacatacaa taatggagta
    34681 gaatatacaa aggatataac tgaagatata actatgggat tacaagattt agaaaaataa
    34741 atacatattt aaataaatat tcaaatagat ttgttcaata aaatagagta taaataaaac
    34801 tgtagtagtt ttacttatac tctattaaaa aattataatt ttaattccat atatctagcc
    34861 ccttcaattg gattataagt ataaggttct atatcataaa aaccaaactt ttcatataat
    34921 ccttgtgcac ttttcatgct tggaagagta tctaatctca tgtgtgtata tccaaatttt
    34981 ttagcttctt caatgatttc ttccaatagt atttttccta tttttaatcc tctaaattga
    35041 tttcttacat ataatctttt taattcacat attttacctt caagtttttt taaagcaaca
    35101 caacctgcca aattttcatc aacaaatgcc aatatcaatg agcctgaagg ttttttatat
    35161 tttcctggaa gtgtttttaa ttcattatta aagtcttgaa aacataagtc tatatttaga
    35221 gaatttgaat attcagtaaa tagaagtttt acattttcaa ggttttctaa atctttttct
    35281 tcaacaaatt gtaaagttat attttgagcc attttatccg ccattatata cacatgttta
    35341 tataatgact tcacctcctt gatatatgta tatttttctt aatatcaatt agattttaat
    35401 accattactt tttcaccacc atagtcaagc tctccattaa actcaaatcc aaaatcttta
    35461 taaagcttaa tagccatttt attgtcttcg aatatactaa gatatagttc atcacagtta
    35521 tattcatttt taagatgatt tacaagaaaa tctaatgatg cttttccata tcctttacct
    35581 tgatattttg aatcaatcat aaatctatca agccaaactc tatcatcttc taaacgacca
    35641 tacatagcaa atcctattaa agaattttca tcatatattc caacaggtac ccaagctgtt
    35701 ataaatttag attcagcaat agaaagtgca ttgctttcta tataatctaa ctgactttta
    35761 tctacagaca aactaactaa atcatgccaa ttatttatgt ctgcaattct tacatttaat
    35821 ttcaagatat taccgccctt caaacttttt gttacaatct aatactatca tattatgtat
    35881 taaaaatcac ttattaagat tttaaataaa ctttttaaat gaataaattc tcaggaaaaa
    35941 ggaattataa atacataata atacagcata ggagtagatg gatatatgaa caaaataaat
    36001 aaaaatttca aaaatatttg gataatatta ggagctgtat cgatttttac catggttata
    36061 atttttaata aaaattgtaa tgttaataaa gagactcaaa tagaagcttt tgcaattaag
    36121 aatcatgata aaaaatttgt atatcctatg ggaaatataa ttggagtaaa agcaaataca
    36181 gatggtgtat tggtcttagg atatgaggaa gaggatgtag actatattgg aggcatacaa
    36241 atcggtgata atatagtaaa aatagataat aaaaggataa aaaatagtca agatgtatca
    36301 gagattttga ataagattaa aaaaagtaaa gttgaagtga cttttgagag aaaaaataag
    36361 tataaaacag aagtaataga gactaaaaaa gaaaatggca agtataaact tggattatgg
    36421 gtaagagata aaatatctgg aattggaact atgacttttt acgaccctag tatggaaaag
    36481 ttcaaagcaa tcggtcatgc cataaaagat tcggatacta atgaactatt aaagataaag
    36541 caaggatata tatataagcc agaacaatta aaaatagtaa aagctagtaa tgaaaaagta
    36601 ggtaaaataa aaggagattt taatgacagc aatttaatgg gtaatttctc aaataattca
    36661 gagctaggta taagtggtaa tattacagaa aaccataata aagagtttaa tgtagcaaat
    36721 aaagaaaagc aattaataga agttggaagg ccgcaagatg taaaaatagg agatgcagta
    36781 attctttttg aggataaaaa caaaaatata acaagctatg atataaaaat agaaagtata
    36841 gtatatgata aaggaaatta tagagatatg gtaataaaag tagtagatga caagttattg
    36901 gaatacacag gaggtatcgt acaggggatg agtggagctc caataataca aaataataaa
    36961 attattggtg caataactca tgtttttaga gataatccga aaaaaggata tggtattttt
    37021 atagatgaaa tgataaaatt gtaggtgagg cattaaaaaa ttttattatt ttatcaatta
    37081 tctaggagga atataatttt ggagtgtcga atatgcttta gagtagataa ttaggaagca
    37141 attgtgtaaa aagtttagtt ttctgtaata agaagatgtt ttttaatggg gggattttta
    37201 gtggaaaaaa tcaaaatagt tttagcagat gacaataagg atttttgtca ggtattaaaa
    37261 gagtatttgt ctaatgaaga tgatatcgat atattaggca tagctaagga tggaattgaa
    37321 gcattagact tagtaaaaaa gacacaaccg gacttattaa tactagatgt aataatgcca
    37381 catctagatg gattaggtgt aattgaaaaa ttgaatacta tggatatacc taaaatgcca
    37441 aaaataatag tactatcagc agtaggtcaa gataagataa ctcaaagcgc aataaatcta
    37501 ggagcagatt actacatagt aaagccattt gattttgttg tgtttataaa cagaattaga
    37561 gagctagttt ctaatagagt tacacaagtt gagccaaaac ctagaccagt tcaagagact
    37621 caaatgacta gaagtgattt tgttaagaat gtaggaaata tagagacaga aatcacaaat
    37681 ataatacatg aaataggagt accagctcat ataaaaggat atttatattt aagagaagca
    37741 ataaaaatgg ttattgataa tgttgagctt ttaggtgcag taacaaaaga gttatatcca
    37801 agtatagcta aaaaattcaa tacaacacca agtagagttg aaagagcaat aagacatgca
    37861 atagaggttg catggagtag aggaaaagtt gacacaataa atcaattatt tggatatacg
    37921 gtacacaata ctaaaggaaa accaactaat tcagaattta tagcaatgat tgctgataaa
    37981 ttaagactag aacatagtat ggttaaataa acaagacata aaaagtaagg ctttttaatt
    38041 aaggcattgg ctataaatgc gtattacaag cagcgaaacg gtataaccac tagggttata
    38101 ccgtttcgct attttaataa atataaaaat tttctttatt atttgcttac tatatcaata
    38161 taataatttt attatactat ggatatagta tgtgtcttta caagttgtaa actgacagtg
    38221 gtttattttt taatataaat attgactttg atgcaggtaa actttgtatt tttaagcgta
    38281 ttatggaata tgttaaataa aaaaatgatg aaatatagta ttgtaaatgc caaagatgca
    38341 aaacaaactt aaaacattta ttttattgtt aagtaatgct ataatataat gtgattttaa
    38401 taatgatagt ggaggtttaa atatgagagt cgaggcccct ataaaagtag atcgaaaaac
    38461 caaaaaactt gctaaaagag ttgaaagtgg ggaaatagca gttataaatc atatagacat
    38521 agatgaagtt gctgcaaact ctttagtaga agctaaaata aaacttgtca taaatgcggc
    38581 tccttctata agtggtaggt atcccaataa aggtccaggg atattaactg aaaataatat
    38641 actaataatt gataatgttg gagaagagtt atttgaagaa ttaaaagaag gagaaactat
    38701 agaagttgta gatggaaaga tatatagaaa aggaaaattt ttaggtgcag gagaagtttt
    38761 agacaagtat gaggtttcac accaaataaa ggctgcctat gagaatttag ctgtagaatt
    38821 ggatagattt atagataata ctatagatta tgctaaaaaa gaaaaaggat ttatacttgg
    38881 agaagtagaa attccaaaag tgaaaactaa ttatgctaat aaacatgtat tgatagttgt
    38941 aagaggtcag gactacaaag aagatttaag tactatgctt tcttatatag aagaggtaaa
    39001 gccagtattg gttggtgttg atggaggagc agacgcttta attgagtttg gatatactcc
    39061 agatgtgatt gtaggtgata tggatagtgt aagtgatgaa gctttaaaaa aggctagtga
    39121 aatagtagta catgcttata cagatggaag agcaccaggt cttaagaggg ttgaggaatt
    39181 aggcttagat gcagttgtat ttcctgcgcc aggaactagt gaagatatag ctatgcttat
    39241 cgcctatgag tataaggctg agttaatagt agcagttggt acacattcaa atatgattga
    39301 ttttcttgaa aaaggaagaa aaggtatggc aagtactttt ttagttagac taaaaatagg
    39361 ttcaaagctt atagatgcta aaggcgttaa tttgttatac agaagtaaat taaaaataaa
    39421 gtacatatgg gcattaatag caacagcatt atttcctgtg ttggtagttg catcattgtc
    39481 accaggagtt caacagttca tacaactaat gcaattaaag tttagagtat tattacagat
    39541 gtaaatttta ggaggataaa tatgcatatt aatatgaaat attatatagt cactataggg
    39601 gctatattta tagctttggg tataggcatg ttagtaggat ttaatttaaa taataatcaa
    39661 caattaagtg aacaacaagc aaatataata aatgatttgg atgataagtt tgatatctta
    39721 aaagaaaaaa atgataaact tgacagtgac ttagcaagtg taaatagaga ttatgaagag
    39781 gcagttaatt ttataaataa aaatgtagat aagattttag ctggttcgct gaatggtaaa
    39841 agtataggaa taatatctgc taatgaaaat gacgattata caaaaaatat agaagacata
    39901 ataaataaat ctaatggaag cattgcattt aatataatat taaaagaaaa cataacaaat
    39961 ccagaaaagt taaaggaaat gtcaactaaa ctaggtacag atgtaaaaaa tgcaaatgat
    40021 gcagttaatt atataattga cactcttaaa aaggaagatg caagtgacat tttaacatat
    40081 cttcaagaac tagatgtaat aaagtttaat tttattggag atacatatct aaaatatgat
    40141 tccgttgtaa tagctggtgg aaatgatgca aaagacagta caaaacagtt tgaaaaaata
    40201 gaaaaatttg tagtttcaaa acttaaatca gagaataaat atttagtaga agttcaaaat
    40261 acaggagtta aaacttctta tgtagaacta tattctaaaa acaaagttgc aactatagac
    40321 aatattgatg aaggtatagg aagtatatct ctagcgattc ttctacaaca aggtaatata
    40381 gttggtaatt ttggtagatt ggatactgca acttcattgt tgccatctat taaatagaaa
    40441 taattttaag gagagaaaaa atgga
//
LOCUS       DAKBNN010000011        39401 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000011 DAKBNN010000000
VERSION     DAKBNN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.011"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            186..1691
                     /locus_tag="OUB02_000618"
     CDS             186..1691
                     /locus_tag="OUB02_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCT8884416.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1805..2503
                     /locus_tag="OUB02_000619"
     CDS             1805..2503
                     /locus_tag="OUB02_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCT8884417.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2788..3876
                     /locus_tag="OUB02_000620"
     CDS             2788..3876
                     /locus_tag="OUB02_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884418.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            4115..5305
                     /locus_tag="OUB02_000621"
     CDS             4115..5305
                     /locus_tag="OUB02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8884419.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            5396..6898
                     /locus_tag="OUB02_000622"
     CDS             5396..6898
                     /locus_tag="OUB02_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCT8884420.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            6970..8226
                     /locus_tag="OUB02_000623"
     CDS             6970..8226
                     /locus_tag="OUB02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCT8884421.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            8398..11172
                     /locus_tag="OUB02_000624"
     CDS             8398..11172
                     /locus_tag="OUB02_000624"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HCT8884422.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11237..11422)
                     /locus_tag="OUB02_000625"
     CDS             complement(11237..11422)
                     /locus_tag="OUB02_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCT8884423.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKKAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            11797..12975
                     /locus_tag="OUB02_000626"
     CDS             11797..12975
                     /locus_tag="OUB02_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCT8884424.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      13102..13187
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            13305..16223
                     /locus_tag="OUB02_000627"
     CDS             13305..16223
                     /locus_tag="OUB02_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HCT8884425.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPMVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      16409..16494
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16594..17256
                     /gene="zmp1"
                     /locus_tag="OUB02_000628"
     CDS             16594..17256
                     /gene="zmp1"
                     /locus_tag="OUB02_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HCT8884426.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(17378..18340)
                     /locus_tag="OUB02_000629"
     CDS             complement(17378..18340)
                     /locus_tag="OUB02_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCT8884427.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVVGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(18510..19700)
                     /locus_tag="OUB02_000630"
     CDS             complement(18510..19700)
                     /locus_tag="OUB02_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8884428.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            20006..20452
                     /locus_tag="OUB02_000631"
     CDS             20006..20452
                     /locus_tag="OUB02_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT8884429.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            20454..21548
                     /locus_tag="OUB02_000632"
     CDS             20454..21548
                     /locus_tag="OUB02_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HCT8884430.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMTKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            21636..22166
                     /locus_tag="OUB02_000633"
     CDS             21636..22166
                     /locus_tag="OUB02_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8884431.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            22493..22852
                     /locus_tag="OUB02_000634"
                     /pseudo
     CDS             22493..22852
                     /locus_tag="OUB02_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(23103..24479)
                     /locus_tag="OUB02_000635"
     CDS             complement(23103..24479)
                     /locus_tag="OUB02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884432.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            24747..25919
                     /locus_tag="OUB02_000636"
     CDS             24747..25919
                     /locus_tag="OUB02_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8884433.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKMVFSGDEEIGHKDSAGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            25933..26580
                     /locus_tag="OUB02_000637"
     CDS             25933..26580
                     /locus_tag="OUB02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884434.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            26582..27091
                     /locus_tag="OUB02_000638"
     CDS             26582..27091
                     /locus_tag="OUB02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCT8884435.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(27464..28576)
                     /locus_tag="OUB02_000639"
     CDS             complement(27464..28576)
                     /locus_tag="OUB02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCT8884436.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLAGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            28874..29140
                     /locus_tag="OUB02_000640"
     CDS             28874..29140
                     /locus_tag="OUB02_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HCT8884437.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            29385..31112
                     /locus_tag="OUB02_000641"
     CDS             29385..31112
                     /locus_tag="OUB02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884438.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            31112..32902
                     /locus_tag="OUB02_000642"
     CDS             31112..32902
                     /locus_tag="OUB02_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884439.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(33064..33372)
                     /locus_tag="OUB02_000643"
     CDS             complement(33064..33372)
                     /locus_tag="OUB02_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884440.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(33634..34182)
                     /locus_tag="OUB02_000644"
     CDS             complement(33634..34182)
                     /locus_tag="OUB02_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884441.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(34285..34956)
                     /locus_tag="OUB02_000645"
     CDS             complement(34285..34956)
                     /locus_tag="OUB02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8884442.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEP
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            35352..36257
                     /gene="garR"
                     /locus_tag="OUB02_000646"
     CDS             35352..36257
                     /gene="garR"
                     /locus_tag="OUB02_000646"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCT8884443.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGFNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            36393..37775
                     /locus_tag="OUB02_000647"
     CDS             36393..37775
                     /locus_tag="OUB02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCT8884444.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKAPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMILLLSV
                     INGIIPLPGL"
     gene            37846..39282
                     /locus_tag="OUB02_000648"
     CDS             37846..39282
                     /locus_tag="OUB02_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCT8884445.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
BASE COUNT        15085 a         4586 c         6663 g        13067 t
ORIGIN      
        1 aaaaaaagtt aatataaaag tgatacaata atatagaagt aataaaaaaa aataattaat
       61 ataaaaatgt gtacaaacta atgaatattg atatagtaag ctatcatatg aaagagtatg
      121 tacacatttt ttttactaat ttttcacaat atgaacataa ataatttata ggaaaggaat
      181 cttttatgaa aattcaaaat actgagtggg gatatattga atggaaacat acttatgatg
      241 aaaataatcc aaaacaagct atgaatatat acattgcagt aactatgcct ggtaagaaac
      301 attttaatca tgttcattat ggtcaagagc agatgatata tattttagag ggagagggtt
      361 tgtatattat aaatggagtt tggaagccat tttatcaagg tatgattttt tatatagaat
      421 ctggtagcac acatgaaact ataaatacag gtgatagaga aataaaagaa cttattgttt
      481 caaataatgt agatgatgta ggagagagtg aagttataga tattaatcct aataattatt
      541 taaagaagac cttgataaat tatagtgaaa gtacattaaa tttgtatgca gcagtagaat
      601 caattcgtgg tcaatttata gacccgttta aaattccatt aattatatat gatgattcat
      661 ggaatatagt tttaaaaaat ccttattttc cacttttttg ttttgaaaaa tgtaatccta
      721 tgaaatttcc tcaaaattgt gattgtatga atcaaaaaag tagcaatcaa tttgtatgtg
      781 aatatgggat aactatatat aatataccaa tactgtacaa aagcaatagc ataggtgtta
      841 taagaggagg gtatgtacta ttatctgatt tgaatttaga tacagagcat aataatttat
      901 atgatattcc agagggagct gctagaagta taaaacgcct cctgaaacaa atatctaaaa
      961 atataataaa tttttgctct tttaatgata taagaaagga tttacaagaa aaagagaaga
     1021 ctatagcaag aacatatcat tatggagagc aacttgaaat gaatttaaaa gtagctcaag
     1081 atatggttac taatttaaga ataaatcatc actttttatt taatacatta aattcaatgg
     1141 caagcatagc actagatgat ggtagttatg atttgtatag tgcaataata gatttatcaa
     1201 gaatgtttag atatacaatg aggtctgatt taagatttgt agaattagaa tctgaaatat
     1261 tatacattaa aaattatttg aatttacaga aattacgtta tggagatgca cttaaagtaa
     1321 aatatcttat tccagagaag ttatataacc tttctgtgcc ttttaacttt attcaaccta
     1381 ttgtagagaa tgcatttaca catggattta gagatattga cacagaaaag cgaattgaaa
     1441 taattgcaag attagattct cagtatgcta ttatagagat tcataataat ggaactatct
     1501 tagatggaaa taatatagac aaaataaagg caggtattcg tagtaataat ggtcatggtt
     1561 tgtctttaat atatacaaaa ttcacatctg cttatggaaa taattttgat atggatataa
     1621 agtcgagtga taatgaagga acttacataa tgattaaaat tcctattgaa aattataagg
     1681 agtatgtata gaacttgtga aaaattaata agttttataa atgttaagaa atatattaga
     1741 tgtttttatg ttgttttata tatcttactt ttttatatta ttaagctaaa agggaggtat
     1801 tctcatgaga agagcactaa taatagatga tgaagaagct gttctaaaaa taataacaca
     1861 ttttatagaa atgaaagata tgccaataaa aattgttggt aaagcaacat ctggtgatga
     1921 agcagtagat aaaatcattg gtttagagcc agacatagtt tttatagata ttcaaatgcc
     1981 aatatacaac gggttagaag taatagaaaa aacatctcat ttatctaaaa aaataaactt
     2041 tgtagtaata actgcattta actattttga atatgctcaa aaagctttga gattaaatgt
     2101 aaaagatata ctccttaaac cattggatat gaaagaattt actaagtctg tagaaaaaat
     2161 tattggatat caatatacca ataatgattt attaaatgag atactggaat atataaatct
     2221 aaactatagc aatgacttaa agttaaatga ttgtgctaga ttatttttaa ccaatccatc
     2281 taatatatct aggattttta aaagtaattt aaatacaact tttatatcat atctaaatca
     2341 tataagaata gaaaaatcta ttgagttact tgaaaatact acaaaaagta taaatgagat
     2401 tgcagaaata gttggatata atagcctaaa taacttttat aaaaacttta agatagaaaa
     2461 aggaatgact ccaaaagttt ataaactgaa cactaaaaat tgaaattgat aaataaaaag
     2521 tcaatataga taattgtttg agtgtgtctg aataagtata ctttaattat gataaaaatt
     2581 aaagcgcttt aaacatattt aataatatta aaccttataa aagctgtacc tatttatata
     2641 aaaaaatata tataaataaa aagaaaaaga tatacaaaat aaaaaaagtg aattaaatac
     2701 tgtttcaaaa agacagaatt ttcatttaat ttactgagag tttatatttc ttaaaggaag
     2761 gtaactttat aatggaggta atttaatatg tcagacaaag tgaatatgaa aaatattatc
     2821 tgtattgcag gcgcttatct agcaacagca attggttctg ggtttgctac tggtcaagaa
     2881 attctacaat tttttgcctc acaagggtat atgggtttaa ttggagcatt aatatcaatg
     2941 gtattgtttt catttcttgg tgctgaagtt ataactagag gtagagaatt aaaacttaaa
     3001 gaaccaacaa gaatttacac tttttattgt gggaagattt taggaaaatt ttacgaatgg
     3061 tttagtccaa tatttttatt tgggattctt atagtaatga tttcaggtgc aggctcaact
     3121 ttaactgaat actatggtat aaatccttac ttaggaagag tattgatggc tgttgctact
     3181 ttaattacag tgtctatggg tttagaaaaa ttagtaagca tacttggacg tattggacca
     3241 gtgatagcag tatttacaat tgctgttgga ttaataagct tgtttaagca ttttgacggt
     3301 ttatctatgg ttggagaagc tatgaaaaat attgaagtta caaaagcatc agaaagtcca
     3361 atcgtttctg gtgtaatata taatacatta aatattattg taatgattgt ttttttaaca
     3421 ggaatgggag cttctattga aagtaaaaag gatgcttttt ggggaggttt attaggtgga
     3481 atagtgttta tgactgctgg tatagttatg tatttggcta tgatttcaga tataggaaat
     3541 ttgtatatga aagaaatacc atcactttat ttagcagata atatatctcc gattgtaggt
     3601 gtatgttttt cagttgcatt aatattaggt atctatacaa cagctgttcc acttttatgg
     3661 tctgtaacca atagaattgt tgaagatgaa catcctaaat ttaaattggt aacaacagta
     3721 atagctattt tggcttgtat aggaggtttt ttaccgtttg gtaaattagt aaacatttta
     3781 tatccatata caggttatat ggggctttta attttagtct gtatggtgta taaagcaatt
     3841 acatgtagaa aaaaatcaac acaaagtcag gaatagagcc acttattaat agtttttatt
     3901 taaaaaaatt ttttatataa tccgaattta aaaagtcaaa atagttaaat aaattgcaaa
     3961 acatgataat atatttaatt aaaataatat agtaaaatat aaatataaac taaaattaaa
     4021 tttataaaaa atatctttat aaagattatt aacttatata atttgtagtt aaattataaa
     4081 aaataaaaaa ataaaatttt ggagaggtgt tagtatgcat gaacttattt taatctcaga
     4141 aaagtataaa gaagatattt tagatttttt tacagatatt catatgcacc ccgaattaag
     4201 ttttaaagaa tttaggacta caaaagctat aaaagattta ttagtgagtt tagacataga
     4261 aatattagat ttaggaatgg aaacaggagt tgttggatta ttaaaaggga agtatgatgg
     4321 tcccacagta gcactaagag gagatattga tgcgttgcca atatatgaag aggttgatat
     4381 taagtataaa tccagatatg atggtataat gcatgcatgt gggcatgata tacacacgtc
     4441 ttgccttgta ggatgtgcat atgtattgtc tcatattaga gatagtcttc atggaaatgt
     4501 aaagtttata tttcaaccag ccgaagaagt caataaaggt gcgaagatgt tagtggaacg
     4561 aggagtaatg gaaaatccaa aagttgatgc tatatttgga cttcataatc atccagatat
     4621 accttgtgga aaaataggtg ttaagttagg tgggttaatg gcagcagtag acactattaa
     4681 gatagaagta aatggatttg gaggtcacgg aggtatacca aacagaacaa tagaccctat
     4741 agtagcatca agtgcaatta taatgggtat tcaaaccata gtaagcagaa acataagtcc
     4801 attagaatca gctgttatat caataggtac tataaatgga gggactgcaa ataatgtaat
     4861 ttcagaaaag gtagatatga caggcacatg tagaagcttt agtaatgaag tacgaaagaa
     4921 aatatcagaa aatttagaaa atatagtttg tgagatagct cgtggatatc aagcaactgc
     4981 aaagctagat tatttatttg atttaccagc tgttataaat agtaaagaga tgtacgcaat
     5041 agcttgtaag tctgtgtgtg atttgtattc agaagatgct atagttgatc caataccttc
     5101 tacaggagga gaagattttt ctatattcat ggaaaaagcc ccaggatttt tttactggtt
     5161 aggggtaggc aacaaagaac aagattgtat atatcagtgg cataatccta aatttaaagc
     5221 tgataaaaat tctatattgg taggtacaaa tgtactatgt cagtctgtta taaattatat
     5281 ggataaatta aaaaataaaa tttaagatag agcattctgg agataaagtt tatgaggggg
     5341 tattagtttg aggaaaatta attcacagga aaaaaacaaa gatagtggat tattgatgag
     5401 ggcactaaat gttgtagaga gagcaggaaa taaacttcca gacccagtaa ccatattttt
     5461 attactatgt ataattgttg taatattgtc agcagttata tctaatttag gagtagaaga
     5521 aatacatcct tctacaaaag aagttgtaaa ggtagtaaat ctactagaga aagagcagat
     5581 tcaatcatat ttaggtagca tagttacaaa ctttcagtca tttgctcctt taggattagt
     5641 actggttaca atgcttggag caggtgttgc tgagaaatct ggatttatgg aagtgttaat
     5701 gaaaaagggt ataagtaaag ttccacaaaa acttgtaact gtagcaatcg tatttgcagg
     5761 tatgttgtca catacagcag cagatgtagg atttataata ttaccaccac ttgcagcact
     5821 tgtgttttta ggaattggaa gacatcctct tgtaggtatg tttgcagctt ttgcaggtgt
     5881 tgcaggggga tttgcagcaa atgttatgct tagtactaca gatgtattgt tagctggatt
     5941 tacaatacca gcagctcaaa tgatggaccc atcttaccaa ggcaatccag caatgaatct
     6001 ttattttgct tttatatcag cgatagtttt aacttttgca ggagcatttg taactgaaaa
     6061 atttatagct cctcgtttta caaaatataa tgattcaaca aatgataagg atatacagga
     6121 attgagtgaa cttgaaaata agggtataaa atacgcttta ttatcattgt tagttgtagt
     6181 agtagtgata gttgccttat gtataggtga caatgcattt atgaaagatc cagagactgg
     6241 ttctatacta tctagtaatg cacctttaat gaaaggtata gttcctataa tcactattat
     6301 atttttaact ccaggattag tgtatggaaa agtaagtaag aagataaaaa gtgacaaaga
     6361 tttagtatct atgatgggta gtagtatgag tgatatgggt ggatatatag tcctagcatt
     6421 tatagcatcc cagtttatta atttatttaa tcttagtaac ttaggaacaa ttttatcaat
     6481 aacaggagca aaattactag cagagtcggg gatacctagc tatggattga taataggatt
     6541 tatattgcta tctggattta tcaatttatt tgtaggaagt gcttctgcaa aatgggcgat
     6601 tttagcacct atatttgttc ccatgtttat gctactagac tttaatccag cattaactca
     6661 aatagcttat cgtattggag atgcttcaac aaatccaata agtccacttt ttccgtattt
     6721 tccagttata ttggcctttg ccagaagata tgataaagat ataggtatag gtactgtcat
     6781 atcgaatatg attccatact cagtggtatt tacattaata gaaataataa tattgttact
     6841 attcatgggt attggcatac cattaggacc tggtggagga atatcttacg ttttatagac
     6901 cgttataata aatttatcaa tgaagattaa actaataaaa tatattcttt aattaagagg
     6961 aggattatta tgttaaaaac aacaaaaatt tcagacccag agttatacaa gattgtagct
     7021 gatgagttgg ttagacaaga acacaatatt gagatgatag cttctgaaag tacagcacca
     7081 acagaagtac ttgaattaag tggatgtgta tttacaaata aaacagaaga aggctatcca
     7141 ggggctcgtt ttcaagcagg ttctgaagaa gcagataaac ttgaaaccct agcaataaaa
     7201 cgtgcaaaag aagtatttgg agcagaacat gtaaacgtac aaccgtattc aggttctaca
     7261 gctaactatt gtgtatattc atcaatatta aaaccaaatg acactgtttt aagtatgcgt
     7321 cttgaccaag gtggacactt gacacatggt agcgcagtta attttttaca tgacatttat
     7381 aaatatgaat tttatggggt agacccaaat actggacgta tagattatga tgctcttgaa
     7441 gcaaaggcaa aagaatgtag accaaaactt ataattgcag gggcaagttc atatccaaga
     7501 ttaatagatt atgaaagaat atcaaaggtt gcaaaagaag taggagctta ctttatggta
     7561 gatatggcac atgttgcagg attagtcgca gcgaaagtaa tacctagtcc tgtaccttat
     7621 gcagattttg tttcttcatc tacgacaaag actttctgtg gaccaagaag tggtattgta
     7681 ctttgtaaag cagaacatgc aaagaaactc gataaaggtg tttttccagg aacattaggt
     7741 tctatacatc ttaatacagt tgcagcaaaa gccttttcac tattatattt aagtacagat
     7801 aaatttaaaa agataatgga gcaagttgta gtaaatgctc agactctagc ttctgaactg
     7861 atttcacatg gttttagtat tgtaagtgga ggtactgata atcatatagt aatggttgac
     7921 ttaagaagta agaatcttac aggaaaacaa tttgaaaaag ctcttgaata tgtaggaata
     7981 acagttaata aaaatgttat acctgacgac ccacaatcac catttgtaac tagtggtgtt
     8041 cgtataggtc ttacatcaat atctcaaaga ggtctgaaag aaaaagaagt tatacagata
     8101 gcagggataa tgaataaggt agcagaaaat attgataata aagaagtatt agatgaatgt
     8161 aaagcagagg cacaagaact tatatctaag tttccattat atccagaagg gtattttgaa
     8221 gattaaatat tttgatttgt atagctcaca atttataaag cctgtaacat aaaatttaat
     8281 aatatataac cactataagg tgttgattaa tcaacacctt attttttttg aaaagtaata
     8341 tacaagctat cataagcata taattcctta tagataatta acaaggggga taaagttatg
     8401 aggtattata ataaacctac caaagaagta ttaaaatact taaaaacaaa tccagaaatt
     8461 ggacttgatg ataatgaggt agaagaaagg aagcttagat atggattaaa tgaatttact
     8521 ataaaagaag gtagaacatt ttgggacgag ttgggagaaa gtttgacaga acctatgata
     8581 ttaattctca taggagcagc agtaataagt tcttttgtgg gagaattaca tgacgcatta
     8641 ggtattttag gggctatttt tattggaatt tctataggca taataactga aggaaaatct
     8701 aaaaaagcag ctcatgccct gtcaaaatta acagaaaata tagaagtaaa ggtgcttaga
     8761 aatgggaaga taattaaaat atcaaaaaat gatttagtac caggagatat tgtatatata
     8821 gaaactgggg atatgatacc agcagatgga agacttatac agtctataaa tttgaaactt
     8881 agagaagata tgctaacagg agaatctgat gatgtagcta agaatgctga tgctgtatta
     8941 gatatggaag tagtttattc taaaacagaa ataatcgaac aggatgcaat acctgcaaag
     9001 caagtcaata tggtatttgg agggactctt gtagcatatg gtaggggcat aatggttgtc
     9061 actcatactg gtgataagac agagatgggt aagatagcac agaatttatc aaatgaagac
     9121 cagcaaactc cacttcaaat taaattaggg aagttgggtg ctaaaatagc aggaatatca
     9181 ggcataatag ctactttatt gtgtatgttt atgattatac agatgcagag aaaaggtatg
     9241 ttaattttag atacaagcag tgtattatct ttcttacaat cattagaacc tgctaaaaat
     9301 gcatatatgg tttgtattgc attaatagtt gcaactgttc cagaaggttt gccaactatg
     9361 ataaatatta ccttagctat aacaatgcaa aagatggcaa agataaatgc attagtaaca
     9421 aagaaagaag cctgtgaaac tataggttct gtttctgtaa tatgttccga caaaacaggg
     9481 actttaacac aaaacaaaat gatggtagag gtagcatatg ttgatggtaa atatataagt
     9541 ggaggagagt atcaaagtaa tagctatttt gaacaaaact gtatagtaaa ttcaactgca
     9601 gatatagaaa aagaagataa ttcatttaaa tatataggaa gcgcaacaga gtgtgcatta
     9661 cttttatatc ataatgataa gaactataat gaaatgagaa aacaaacgta tttgatatca
     9721 caaataccat ttagttcaga agaaaagaag atgtcaacat taattcgtca agaggattca
     9781 gatatattac tttcaaaagg tgctccagaa gtattattaa aaaaatgttc ttatgtacaa
     9841 caaggaaaaa atattgttcc aataactcca aaagtagaaa aaagtatttt agatgagatt
     9901 aagaagttac aaattaaatc tatgagaaca ttaggatttg cttataaaaa aatgagtaat
     9961 tctaaaacag aagttgcaat gacttcagaa ggtgaattga atttaatagg aaattcaaga
    10021 agttatatga aggaagataa tttagttttt agtggatttg taggaatagt ggacccattg
    10081 agagaagggg tcaaagattc cattgataaa gcatttaatg caggagtaga tgtaaaaatg
    10141 cttacaggag ataatataaa tacagcaaca gctattggaa atgaattagg actattaaat
    10201 gatgggaaaa aggcagttga agcaacatat atagatgtat tgacagataa agaattgaga
    10261 gaagaaatca aaggaatatc tattgttgca agaagtaaac cagatacaaa aatgagaata
    10321 gtttcagcac ttcaaaaaag tggcgaagtt gtagctgtaa caggtgatgg tataaatgat
    10381 gccccagcac ttagtcaagc agatgttgga attgcaatgg gtatttcagg aacagaagta
    10441 tctaaaaatg cagcagatat aatattgact gatgatagct ttagtactat agtagaggga
    10501 ataaaatggg gaagaggtat atatgagaac tttcaaagat ttatacagtt tcagcttact
    10561 gtaaatatag tagcatttat aattgctata atatctcaac ttactggtaa ggatatgcca
    10621 tttactacaa tacaattatt atgggtaaat ataataatgg atggtcctcc agccctagca
    10681 ttaggattag aacctgttag agattatgta ttgaaaagaa agccaataaa tagacattct
    10741 ggaataatag ctaggtctat gtttgtaaat attattataa atgctattct tataattaca
    10801 attgtattta ctcaatcagc ttttaatata ttgggagcta catcagaaga acaaggtact
    10861 gttatatttt ctttatttgc attcagtgcc ctttttaatg cattaaattg tagagagttt
    10921 ggtttaaata gtacaatacc aaatttcttt aaaaataaac tagcattgca aataatagta
    10981 gttacaggga taattcaaat aatatttaca caagtgttcc agagtttctt taattctgtt
    11041 tctttggact ttgatatgtg gataaagata atactatttg catccactat cctattgtca
    11101 aatgaatttg taaagctaat tcttagaaca atgaaaaata gtagaactat gaactttaat
    11161 agtaaaaatt aattcttatg aaaccgtaaa gtactatata ttgtaacttt gcggtttttg
    11221 tttgtttaat gttgttttat attaccattt tcatatttaa ttttttatta atctgaacac
    11281 aatcttccat tgtaattgct tcatctgtgt caacacattc aaagtcaata tctaattttt
    11341 gtaatatttt gtatgccttt ttttgcaaag gagtatcaaa tttaataggt gattttgtgt
    11401 aaatatcact gataaaaaac atgatttatt tcattccttt ccatttaaaa tcttatagtt
    11461 atagtgtatc ataaggttat aaaatataga taaatatagt ataaatttaa aatatgtaca
    11521 gacaaaacaa aaaaatcata ttctaagtaa tacttgggaa aaatgatatt atcataataa
    11581 ataatctttg taaaaactta tatagaatct attaaacaga cactaagcca taaaaacttt
    11641 actaaatata aaattcgtat ctaaatagat atatagaata atattttact gaagtagagt
    11701 gatttttaag aattttatat ataaattatg ttatcataaa taaagagata gattataaaa
    11761 tttgttaata aaataaaggg cagggaggta agtttcatgt tttccaaaat cctagtcaaa
    11821 acatttatta gagatagcga aaacgttcaa aatacagatg ttagaaataa gtatggttat
    11881 gtagctggtg tagtaggtat attatccaat ttacttttat ttgtgataaa ggtattcata
    11941 ggaatgctca catctagtat agctataatg gctgatgcat ttaataatct ttctgatatg
    12001 gcatcttcag caataactat gataggtttt aaattagcaa gtaagccagc tgataaagaa
    12061 cacccttttg gtcatggaag aatagagtat ttatcagcat taatagtagc atttatggtg
    12121 atgttggttg gacttcaatt tgtaaagtca tctatagaaa ggatagtaaa tccaattcct
    12181 gtaaaatttg aagtaatacc acttatactt ttaattgcat ctataatgat taaaatctgg
    12241 ttgagtcgtt ttaataaatt tatgggaaat aaaattgatt cttcagcctt aaaagcagtt
    12301 tcacttgatg ctttaggaga tgtatttact tcaagttgtg tagttatatc atttattgta
    12361 gctagattta ctaattttcc catagatgga tatgttggta tcgtagtatc attagtgata
    12421 ctttatgctg gtttctctct tgtaaaagat actataaatc ctcttttagg ggaagcaccg
    12481 gatgaagaaa tggtaaattc tattatagag ctattgttat cttataaata tataataggt
    12541 acacatgatt taattattca taactatgga gttggtagat gtattgcatc tatacatgca
    12601 gaaattccat ccaacataga tataatggaa atacatgaaa ttatagatac tgcagagaga
    12661 gaaatatctg aaaaattaga tatatatcta gtaattcaca tggacccaat atgtcttgaa
    12721 gataaagaag ttatgtcagc caaaaaagaa cttgaagaaa tacttaagaa aaatagtctt
    12781 gtaaaatcta tgcatgattt tagaatagtt ggaaaaggaa gcaagaagaa tttaatattt
    12841 gatatagtag ttaatccttc cgaattttcc aaaaacatgt cagaagatga cttaaaagaa
    12901 gatataacaa aattagttaa ggagataaat cctgaatata attgtgtaat tgttgtagac
    12961 aaggatttta tttaattatt aattaatctc atcataaggt aaaaaatgta aaaaggatag
    13021 acaatagtat agaggcataa tataatagaa gtatataatt gtatttttaa tataaataaa
    13081 gcaattttaa ttgtttttat gaaatattat agagatgttg aagtatattc tattattggg
    13141 caccttatgg atatactgag tcagtggtgc aaccggctat gaatataaat ttatttattt
    13201 tcatagcttt ttttgtataa ttttttagta aaaattactt tatatacgat attaatggaa
    13261 gattagatta taaattatag gtaacctaaa ggggtgagat ttacatgaag aaaggaaata
    13321 gaaaggcatt attaattagt ttaattatga ttttaagtat ggtggtgtct acaatatatc
    13381 caactgtatc ttatgcttcg gaattaggag agaatagtca gattcaaagt ggttcaacta
    13441 attcatcaac tggagaggag aaggaaagcg acaataaaaa gccagaacag actccagaga
    13501 aagataaggc aacagataat aaaaaaccag aacagacccc agaggaagaa aaaccaacag
    13561 ataataaaaa accagaacag accccagaag aagataagtc aacagataat aaaaagccag
    13621 agcaaactcc agaaggagaa aagccaatag ataataaaaa gccagagcag ataccagaag
    13681 aagataagtc aacagataat aaaaagtcag aacaagcttt agaagatgaa aaaccactag
    13741 ataataaaaa tacagaaaaa actccagagg aagataatct attagaagat gaaaatttat
    13801 tgaaagtttt agaagaagaa cttaatgaag aaaatgaaga ctatggattt gttgtaaaaa
    13861 ttaataacaa tacaatagaa acagaatcaa tgaaaaaaat atcatttaat ttaacttata
    13921 ctccaacatc aaaaggaata caagcaggag attcaataac ttttaaggtg ccagatgtat
    13981 tcaataaagt taatttagat tatacaagtg aatgctttga taaaacagaa agtaatggag
    14041 agtatacact tacatttaga gaattaccta atggccaaag tgtaatgcaa ggtaaaatag
    14101 gtttggaagc atatgttaaa aaggttgatg aagatactaa tgcaaaaata catattgaaa
    14161 ctactggaaa aatagaaagt ggaagtggag atatagatgt agaaataaaa cctggagata
    14221 aaacggatgt tccagatgct aaaggaacac ttaaaaagct tgttgaagga aaaaaatcaa
    14281 ctactgtatt tatgccagtt aaaaataaag atataaatta cagtatacaa gtaaatgaaa
    14341 agcaggaaga gttaaaagat attattttgt atgatgagct tcctgaagga ttgacattga
    14401 taaatggttc tgtaagtgta gtaacaagtg atggaaaaga agttagtgat tttaatattg
    14461 aacagagtaa aaattctatt tcagttaatt ttggaaatat agataaatcg tatactgtta
    14521 agtataaggc tagaatcagt gacaaaaatg caaaacatgg taataaatat aagaatgtag
    14581 ctaggattga atctgatggt aagaagatac aagaagatga tgctactgta agtatatttg
    14641 atagaggaga tgattatctt cttactaaag gacattcagg agcaacaaat attactcaag
    14701 ttggtcaagt aataaactat cagatttcaa taaatgatga taaatcacca ataagtaatg
    14761 ttgttataac agataatata ccagaaggca tgagattaac tacttcagga gaagctgggc
    14821 atgattttag agttgtagaa attccaatga atgggagctg gactccttgg agtaaagaaa
    14881 aaattgccaa taatatttca tataaagtag aagaaaaaag aaatgaaagt gggcaagtag
    14941 ataaagttat aactggattt acaattaatc tcagtaaaga agaagtggaa tcaaagtttt
    15001 ttatagctta tacattaaag gtaattagta ttgaagatag ctatataaat agagctgttc
    15061 ttgatgcaaa taatagtgaa attgataaaa atgatgagat aaactttaag aaaaattctg
    15121 gattaataag cgcaaaaaaa gaagttgata aaaaagtttt aaacagtagt gataaccaga
    15181 ttgttaaata caaaatcaat atgtcaactt atggagttta tgatgcagga caagtaaatc
    15241 ttttagatga agtaaactca gttctagaaa tatctaatat aaaatattct gataatcttg
    15301 aacttaaaaa agaagctgga gatggaaaaa atacaataag attagtaaat aaatacgaat
    15361 ttaaacagat aaaagaaggg gaacctgtgc aaagttgggt tacatttgat gcaaacttca
    15421 caaatgtaaa agttggggag actatacgaa atgtggctca gattaatggt tcaagtcctc
    15481 caggagtaga gactactaag caaggttatg cttttgaagc taaaaaagtg gatgctttag
    15541 ataaaaatgt actttcaggt gctaagttca atttagaaga tgcatttgga aatatagtag
    15601 ttaaggattt agttagtgat gaagatggta taattcaatc tagtgtaaaa aacccaggaa
    15661 catattattt agtggagata atggctccac gtggatatga aaaactgaag gataaagtta
    15721 aggttgaaat tggtaatgaa gatattggta agatagtaga tattggaaat atagaaaatc
    15781 taaaacaaga aaatccacca gtaaatccac caatacctcc agatacagat gaaccaatgg
    15841 taaatccacc agtaccacca agcacagata aaccaaggaa accatcaagt tcatctgata
    15901 cagaggatac tatagttata aatccaccag taccaccatc agaagatata ataaatccgc
    15961 caataccaga ggtattgaat ccgccagtac caccatcaga agagatgata gaaactcctg
    16021 ttaaacagat tattccaata ccagaagtag taaagccatc agtgtcagaa gaaaaaaata
    16081 ataaagttaa agatgatact ttggtaaatc caccagtacc accaaaaaca ggggatagta
    16141 ctacaattat tggagaaata cttttggtta taggagctat tgtaggactt atcgtattaa
    16201 gaagaaataa aaatacaaat tagaccctaa cattataaaa aatataagtt atattaaaaa
    16261 atatcttcta aaacaatatt taataatgaa aatattgtta gaagatattt ttttttgaag
    16321 atttatgtaa aaaaataata actttttaga aaaaagttcc aaagggatat agacaattaa
    16381 aaagatataa gctataatat agatataaaa ttaaatgagg caaaactagg gaaacctagt
    16441 cacgcaaagc tataggggct aaagtttagt aaaccatgcc agccagttgc cgaaaagagt
    16501 aaaaatttct ttttgtatat acaaagatac tcttgggtat cttttttatt ttgaaattta
    16561 ttactcccgg cctaactcaa aggagggaat aatatgagac caagtaaaaa attattaata
    16621 gctataatat caatattctt aataagttca gttccagtaa gtgcacatgc agatagtact
    16681 actatacaac aaaataaaga cacacttagt caaatagtag tttttccaac tggaaattac
    16741 gataaaaacg aagctaatgc tatggtaaat agattagcta atatagatgg aaagtattta
    16801 aatgcattaa agcaaaataa cttaaaaata aaattattaa gtggaaagtt aacagatgaa
    16861 aaagaatatg cttatttaaa aggtgtagtt cctaagggat gggaaggtac tggaaaaact
    16921 tgggatgatg ttccaggttt aggtggaagt acagtagctc ttagaatagg atttagtaac
    16981 aaaggtaaag ggcatgattc aataaattta gaattacatg aaacagcaca tgcaatagac
    17041 catatagtat taaatgatat atctaaatca gcacaattca aacaaatatt tgctaaagaa
    17101 ggtcgttctt taggtaatgt aaactactta ggtgtatatc cagaagaatt ttttgcagaa
    17161 tcatttgcat attactattt aaatcaagat acaaatagta agttaaaatc agcttgtcca
    17221 caaacttata gctttttgca aaatttagct aaatagagtt ttaataaatt taaaataaat
    17281 ataaataaag agatgctatt ataatggcat ctcttttata ttgcgtttaa atctaataaa
    17341 ttttgtctta tgtataacta tgatagatta tcaattacta ctccttatac aaggcttcaa
    17401 cgtgtttatt atgcatttga gtaattatcg taggtataat agaaattatt gaaccaatta
    17461 ctatcaataa aattcctaca accatgttta tagggataat ttctttaagt attaataatg
    17521 ctagtattgg agatattact ggtttaaaga agaatactaa agatgtaaga ctagtagatg
    17581 tagcttccat tgccataaag taaagcgcat aaccaacacc tgtaacaaat acgaatacat
    17641 aaatcataat gggcatattg tgtaaggtat atccactaaa aatagggata tttgcaaata
    17701 catgaagctt atttttattt aatatatctg cgataaaatt aattttagtg agtaaaacca
    17761 atatcaacat ttccaaacta ccaaatataa agctaaagca ggttacaact attccaccaa
    17821 attttaaggt ttttctttta ccaaatacag tatacattga aaaaataata gcagaagata
    17881 atgttaagaa tattccacta agagttagtt ttgtatgcaa tggatttatt ataactataa
    17941 ttccaagaac ttctaatata agtgaaatta cattgtgcct atatattttt tccttcaaaa
    18001 ttaagtaagc taaaagcatt acaaagactg gattacaact gaacaaaaca gcaactacag
    18061 atgcctttgt gtttagaaca gcaagttgat acaatgacat acttactact acacacataa
    18121 aacctaaaaa tgcaaagtat tttaagtctg attttgtaat agaaagacta agggctctta
    18181 atctttttac agcaaaaggt agtaagacta aaccgccaac aaaaaatctt gataaagtta
    18241 tttgtaaggg attaaagtca ttagtgacag tttttagact aatttccatt gaactaaaaa
    18301 ataaggtagt cagtattata taaacataac cttttttcaa gtcaaacacc tccttgtaat
    18361 ttatattagt actatagata agaatattca agcatattgg aataatatgg tatgtataaa
    18421 atatagtaca taaaattata aactacctta atatagaatg taagtattct atactaaggt
    18481 agttaattat ttgaattact ttgatttagt tattttaaat tatataattt ggctaatttt
    18541 tcaaatgctg gtaaagaact tttaacttta tcataagata cacaatatga tactctaaaa
    18601 tgacctggac aaccaaaaga agaacctggt actaataata aattaaattg ttttgcatca
    18661 ttgcagaatt ttttatcatc ttcaattgga gcttttggga acaaatagaa tgtaccatta
    18721 ggtttaacac aagaatatcc catatcgatt aaactattat atagaaggtc aaaattcttt
    18781 ttataaatat ttacatcaac ttctgcatct aaacttcttg caataaccct ttgaaaaaga
    18841 gaaggagcat ttacaaatcc aagtattcta tttgcaacat ttaatgaaga cataacatca
    18901 tcaaaatcat ccatttgccc attagcaaca atatatccta tacgttcacc aggtagagat
    18961 aatgatttac tataagagta tcctataaat gaatttctat agtattttag tacacaagga
    19021 actttggcac catcataaat tatttctcta tatggctcat cagaaattag gtatatgctt
    19081 gtattaaatt ctttttgttt tgaatccaat aattctccaa gattttttaa taattcttca
    19141 gaatatatga caccagtagg attatttgga gtgtttatta ttaaagctct tgtttttgga
    19201 gtaatagcat tttttagagc atctaaatct ggctcaaaag tctctatatt tgtaggaact
    19261 tctattaatt ttccatcata gttttctgta taatttttat actcaccaaa atacggtgca
    19321 aaagcaataa cttcatcacc aggatttaaa agagttttta aaataatatt tagaccacca
    19381 gcagcaccac aagtcataat taaattttct cttgtaagat ttaatccatc ttttttattt
    19441 atgtgttcag caatagcatc tcttacatcc tcatatcctg agttgttcat atatccatga
    19501 actaaatttg gagattcttc atttaaaata tcatttataa ctgctttaat agtctctgga
    19561 ggttcaacac ttggatttcc tatactaaaa tcaaaaacat tttcctcacc ataaatatca
    19621 gaaagtttct ttccttcttc aaacatagct cttataacag aactatttgc aactaaagtt
    19681 tgcattttat tagatatcat atgtaacact cctttttata gatttatatt gcttatgtat
    19741 atattaccat atttttactg aatttagaca attttcgaaa aattacttgt ttgaaaatat
    19801 aaacttttat ataaaatata tttatgtaca gtgcatatta aaaattccaa tttctaattt
    19861 tatcatgatt attttatata atagtttttt aaagaattaa taaaacatag atttagagaa
    19921 taaaatattc ttgactatat taattgttta atataatata gttgatatag tcaaacatat
    19981 tataattata gaagggaaat ttaagatgga ttttaaaaat ttacagtatg aaagtttgtc
    20041 atatattatt tgtaacttgc aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc
    20101 atataaactt acgaatggtc tatatttcta tttaatttat attaataaaa ataggaattg
    20161 ttctttaaat gatgtttcta cagaatttga agttgataaa gctcatacaa caagaactat
    20221 atctaggtta gaacaagatg gatacataga aaaaatccaa aaccctaatg atagtagagc
    20281 ttttcagttg agagtaacag ataagggtga ggaagtatta ggggatataa aaaacatatt
    20341 ttcaaagtgg gataatcaca taaaaaaaga gttctcggat acagaatata aagaactggt
    20401 aaagaattta catgtggtaa aggatataaa aacagctgtg gaggaagaat aggatgtatt
    20461 gtaatttatt aaaaccaatt aaaattggaa atttggaatt aaaaaataga gtatcttttg
    20521 cacctacaag catgggtctt aaattggaag aaaaaattaa aaagttttct gatattgcta
    20581 agtcaggagt agctctgatt actctaggag atgtatctat tagacctagt tttcataagg
    20641 tagctataag cctatcggat gaagatggag tcatgaaata caagaaaatt gtagatgaaa
    20701 tacacaattc tggtgcaaag gtatcagcac aactattttg ttcagattat gatgttaatt
    20761 taattaaaga tactatgaag atgggaatta cttcacatga tgagataaaa aaaataatga
    20821 atgacggagt aaaagattac ataacaaata tgaccaaaga agaaattaaa aatattataa
    20881 atttatttaa agtaacagca ttgaatgcaa aaaaagcagg ttttgacatg attcaaattc
    20941 atggtgatag gcttgtagga agtttttcat caagtatatt taataataga aatgatgaat
    21001 atggtggaac gtgtgataat agaagtagat ttgcaagcga aataattagt agtatccgag
    21061 atgaagttaa ggacattcca atagactata aatttgcaat aagacaagaa aatcctcatt
    21121 atggaaatgc aggagtttta ctttcggaag ttgagtattt tgttaaaaaa tttgaatccc
    21181 taggtgtaaa cagttttcat gtaacacttg caaatcattc aaaattagaa gatactatac
    21241 caaccaataa tcatccttac tttaaagatg aaggctgttt tttatattta gctgatgagg
    21301 taaagaaaca cacaaatctt ccagtatgtg gggttggaaa attgtctagc ccagatttta
    21361 tagaaagtat tatttcaaat aatagagtag atatggtttc tatgtcaaga caattactag
    21421 cagatagtaa ttggttacag aaagtaaaag atggaagagt agatgagatt aaaaagtgct
    21481 gttattgtaa taagaagtgt gcagatgctt tacaaacacg ctctcagttt ggatgtatac
    21541 tagattagaa tatccttata aaaataaata atttgttaag gttaatatat ttttggtgat
    21601 ttagataagt atttagataa agaggaggag acatcatgaa aagtttagtt ttatattcaa
    21661 gtttaactgg aaatactaaa aagattgctt atactatata tgatgaaatt caagaagaaa
    21721 aagacattaa agatgtaaat gaattagttg attatagtat tgactatgaa aattatgata
    21781 tagtattctt aggctattgg gtggacaagg gaatttgtga taaaaattca aaacaagtgc
    21841 ttgaaaatat tcacaataaa aagatagcac tatttggaac aatgggagca agtaaaaaag
    21901 gaagttatgg agctagtata atagaaaaga tagaatcaat tatcccaaaa gataatgaaa
    21961 tattaggttc attcatatgt caaggtaaga tagcagaagg attgaaagca aaatataaag
    22021 agatgctaaa actttctcct gacaacgaac atattcgcca acaattaaat aaccatgagg
    22081 aatctcaaag tcatccagat gaacaagaaa tatatgaagc cagtatgttt gcaaaaaata
    22141 tgatgattaa agctagtatt gtgtagtagt taaacaataa gttcatataa gatatagaat
    22201 aaataattta gtaaaaaagt atagcaaata atttgctatg ctttttttat gaaatcaaaa
    22261 aaataataaa aatacttgac tgtaaacctt gtttatacat tattttatag aaaataagat
    22321 agttttaaat ataaaaaaag atatattatt gaaaggagat attattgtat gaaaacttat
    22381 aaaagataat tagtttgatt ttaagatata ctaaatatac aattttatag agtgaaaata
    22441 tttaagacta tattatagtt ataaattaat agaatagaga ggtagtgttt aaattgcaat
    22501 aacagtattt ccaaagttgc cacctcaaat tcctatccaa tggaataatg gtgtagctaa
    22561 ttatgtagat aaaaaattta tttatgccta ccctgtcatt tgtgttgtat tcagaatact
    22621 tttacgtcca tttatatgga gatggttaca aatacatgct ttttatagtg atactattgc
    22681 agattattta acgaatttta tgtgttttgt agcgttgtct atagaaatct tctctatact
    22741 ttttatttac aatattgcca gacatgttac aataattatt tttatagata tacttgtatt
    22801 tattgcttta ctgataatag gtatgtataa attatttaaa aaatacaatt aatttttaaa
    22861 aattaagtaa taagtgatag tgtaaaaagt agccactgca aattatggat tgtagtggct
    22921 attttattat ttgtatatga aattattttg tttttctaat aagttacgat atattatggt
    22981 ttatttcact gataaaaggt ttaggtattt taaagtaaaa agttgtatat tctaataaaa
    23041 tttaaacatt ttgattcttt aaaattagag aaaatacatt gcttttatta aaaaattaaa
    23101 tattaagaat ttaatttctg catcttattt agaaaagata agaaatcaac tttatatatt
    23161 ttttttggcc ttcctctttg attatcgagt tttgtattta caatagttgc aattcctgct
    23221 ttatataaaa tactcaatat tctattagca cttctaagag ttatgttcat atagtctgct
    23281 aatatttctg ctgacacatt atttgaattt ctatcattaa tcatacacat gattttagat
    23341 acatttgtat tagatagagg tataaaagaa gcaactttat tggctattga gctatcttca
    23401 atattattag aatttttatt tgaatataaa ggacctattg tatcattagt atcatttact
    23461 ataaaagtac agtttccatt caaagaagca gatttaccat tagcttgacg agcattttgc
    23521 tctgcatgaa tatttgtatt tccaatgccc caacctatat ttaagctata tttaatttct
    23581 tttaaatctt ctgaaataaa aaaatctgta tagttattgg tcatattgtt taacatttct
    23641 ttagtagtgt gtatctctat tttcatatca ttttttttga cctgaactcc atagaatcca
    23701 tgtcttgata gagaatttaa tatagtgtta tatatttgat tttgtatctc atcattttta
    23761 aatatcaaat cttcatttat gtactctgaa ctatttactg taactataca agttgcagat
    23821 ttatttttgt ctaagttttg taatgttatt tcattcacca gagatttaaa tgtattcagg
    23881 attgattctg tagatggaac catgtaaata caatcatatc caagcttatt aagttcacaa
    23941 ctaatgatgt aatttctagt aaaagaaagg ttaatcttat tatctcgcaa taagtctaaa
    24001 tgtatttcca ataatttatc atttaatttt ttaaagtcat atacaggttg agggatatta
    24061 aaatctttaa ttgtatatgg acatttatta gagtctatta atgaatatat atcataataa
    24121 ttgttgtcct ctcttaaaaa atccataaat gattttgtaa agtcaatatc tttttggcta
    24181 tttaataatt tgaataagta tttataaaaa tctctttcat caagctttaa aaaatttaat
    24241 ggaactttta tgtcttttat atttattata agttcactat atggaactat accactgcat
    24301 acaaatccat caaaattatc tacttgctca tagtatatat taggtaaatt attaaaagga
    24361 ttctcccctt tgagctcaat aaatgtaatt tcacaagtat catttaattc tttaagagct
    24421 tgtttaaact tatctgacat aaaataagat gttgtcaaaa aaccaatttt atatttcata
    24481 aaacctcctt taaaatttcg agagtattgt aactaatcaa tcaatttata acctcatttt
    24541 ataatagtag ataaaacgcg tcaatttatt ttacaaaagg ataattgaaa gaaaaatcag
    24601 aaaatataaa aaagactgaa aaatatgttg actgtcaaga ataattgtgc tattttgtaa
    24661 ttaaggacta gtcttttaag atttcctaaa ataaacttag gttttaggaa agaaatacat
    24721 tttatttgaa aataaaggag aagattatga atacatattt aaatgaaatt tctaagtttg
    24781 ttgatgagaa tagagaagaa atagtttcat tatggaaaga gattgttaat atggaaagct
    24841 atacacattg taaagaaagc gtaaataaat tggctaagag attaaagttt gagtttgaaa
    24901 aagaaggttt agattgcgaa ttagtagatg ttggagataa tggtagtaca ttgataggta
    24961 cacttggttc taatataaat aaaaagccta taattttttc aggccacatg gatactgtct
    25021 ttgaaacagg tacatttgga gagaatccat ttaaaataat agaaggaaaa gcatatggcc
    25081 caggtgtttt agatatgaag ggtggtataa taatttcctt atatgttatt aaagctttaa
    25141 ataaaatagg atacaaagaa agaccaataa aaatggtatt ttctggagat gaagagatag
    25201 gacataagga ttcagcaggt gcagatgtta tattgaggga agcaaaaggt ggtctttgtg
    25261 cttttaatat ggaaactgga ttagtagata atagtttgtg tgtaggtaga aaaggaagaa
    25321 tcggatgtaa tatacatgta aaaggtgtag aaacacacgc aggtaatgat tttgaaggtg
    25381 gaagaaatgc tatagaagaa atggctaata agatactacg catacaaaaa ttgactaatt
    25441 tagatgttgg aactacagta agtgtaagtg tgattaaggg tggaagagtg gaaaattcta
    25501 ttccagaaga ttgtagtata aaagtagacc ttagatttga aaaagtagaa gaaatggaga
    25561 atgttaaaga acaagttaga gagatatgta aagaaactta catagaagga acttctacag
    25621 aagttaattt tataagtgaa atgatgccat ttgaaactac agaagatgtt atgagatttc
    25681 atacatttgt aaatgaagtg tcaaaagaaa atggatttgg agaattaaat gctaagagac
    25741 ttggaggtag ttcagatgca agttatttaa ctattgcaaa cgttcctaca atctgttcat
    25801 ttggtgtaag aggggaatgg aaccacacat caagagaata tgcagtagta gattctatgt
    25861 ttgagagggt gaaattaatt tctacagtta ttttaaactt agataaattt gaggtataaa
    25921 gggggatttt atatggatgt aacagctgga gataataagg taacatggaa agggtattta
    25981 tctttatttg tattgatagt attattttca ggagtgttta aagatagtca aggcttcctt
    26041 aaagcattag attttgctaa tctgacaggt tcatttggta ctattgcaga tggtgtagac
    26101 tttatgggta aaggaggaac gggagcaaaa gatggtttct tatttgctct gactttgatt
    26161 ccaactgtct gttttgctgt aggtcttata gatgtagttg aatctatggg aggaatgaaa
    26221 gcggcatcaa agatatttaa tccattgctt agacctctgc ttggtatacc tggaatagca
    26281 ggaatagcat ttgtatcttc atttactagt tcagatgtag cctcaataat gacgaaagaa
    26341 ttatttgaaa gtggggaaat aacagatgat gaaagaacta tatttgtagc atatcaatat
    26401 gctggttcag ctgttatact gaacacaata aatactcaag ctccactttt accaatagca
    26461 ttattggcat taggtccaat aatccttata gagatattct gtaaaatact tggtgctaat
    26521 ctagttagat taatcatagg tgtaagaaat aaaaagaata aggctaagga ggctgtgtag
    26581 tatgagtcaa caaaatactg gaaatgcaat tgtaaatagt aaacctaaga aaactgctgt
    26641 tgagatattt atggggggag caaaaaaagg tttttacata ggtgttgaac agatattacc
    26701 agccatgata atgggttatg taatagtgca gtttttacaa ttaactggtt tagttgatat
    26761 tttaggaaat atttttggac cagtaatggg tatttttgga ctacctgggg aatctgttgt
    26821 agttttgata gcagcattct ttgctaaggc agcaggagca gctacagctg caaacttatt
    26881 tgcacaaggt ctaatcactg caccacaagc aaccatatta attatgccat gtatgttaat
    26941 gggtactcta gttggtcatt atgccagaat agtgcttgta gcaaatgcca gtactaagta
    27001 tagaatgtta ctgttggcag ttcctatatt tgatgcaata gtggggatgt taatagtaag
    27061 agttttactc actgttatgg gattaatgta aataacataa aattatagcc taattataaa
    27121 tgatatataa gaattaatat atgcaagaat tgatgaatag taattgatat atagtaataa
    27181 aataatatag taattaatat gtatattgta tatcaattaa aaaagaagag agtatctcaa
    27241 agtgaacttt ttagttcatg agatactctc tttgtatgaa atcaagtata ttttcataat
    27301 ttcaacaatg aaagagaggc ttatctctat tttgagacag cctcttcttt taaatttata
    27361 tttctagaat ttatgttttt tatttatcca acattaacat tagttattag aaataatttt
    27421 aagaaaaata tactttcatg ttaatatcag aataaatgtg tttttaaagg atgatttgtt
    27481 ttgaattttc acgaatatct acttctatga ctttttctat tgtatctcct ctaaaaccta
    27541 ataaattact tgtcaagttt gcagtggaac aagaatgatt aggtataatt cttattctat
    27601 caccaatttt tatatcagtt tcaccattta ctttaatttt tccaacttct tctgaaagtg
    27661 aatcaatagt aagttctgga tgtccacata ccctaccata accaacaata ctactatttc
    27721 catgtgcacc tttatcaagt ccaaggcact tacttccaca atcaattaaa tagtatccac
    27781 tgctattttt tgagattaca gtgcacacaa cagtaagagc acacatatct tcagtagcac
    27841 aacctaaacc tatctgtata gcatcatagt atacatagtt tccaggtctt acagcagtta
    27901 tagttttaga agcagcttca gattcaactg ttggagtact accagaagcg acaatttcta
    27961 tattaaatcc attatcagtt agagctttgt atgctttctc catagaagaa tcttcttgaa
    28021 cacaaacatc tggaacacct tctggagaac tacaaccata cacttgacct ggatgcgaac
    28081 ttataccaac aatcttgatg ttttctagct tagaaatttc agtagcaagt tcaaccactt
    28141 tttctggttc aaccccaaaa cggtgtaagc cacaatctac aataataagt agcttgcact
    28201 ctatttttga tttagaaaaa gcttcactat aagccttagc acattctata ttatctgtag
    28261 cacagattaa atttattttt gatgccatag tcacaattct atttatattt acttctccca
    28321 tgtaaggata agccaacata atatcttcaa aacctgcttt tatcagagct tcaccttcat
    28381 caagtgttcc agctagaaaa ccactagcac ctgcattttt ttgaagtctc gctatgtaag
    28441 tacttttatg tgtcttaagc ataggccaaa gttgtttatt attatttgta caaatttgtt
    28501 gaattttatc tatattcttt tctaactggt ctaaatctag caaaaaattt ggagtagcga
    28561 cttctgaaat tttcatgtta tttaccacct ttcaacaaaa gtaaattaac tataagtatt
    28621 ataattatta tattagtaat atcggcttta taatattaat agcttataat tatattatag
    28681 aactagcgtt aacagagagc aatatttatt tgtaataatt agaattttca ataagttagt
    28741 gtagttttaa attatttata taatatattt ttaattttat gcaaagttta tttaaattag
    28801 tttgaggatt tattaatagg gtattattga atgagtaaaa aagtgatgtt tacatagaat
    28861 aatcacacta taaattaatt tagaacctaa aaaattaatt gagcaaattg gaaaagagaa
    28921 ggataaaaaa gatatttgga tagttggtgg aggcgatata gtaaaattat ttatgaaaga
    28981 taacttaatt gatgattatt atatatatat attgccaata atattgggga gagggatttc
    29041 actatttaag tctggtatag acaagactaa tttgaatttt aagaaagcta gtaatattgg
    29101 agaacttgta aaattggaat acagtaaaaa acaaaaataa attacatgcc aatttgttac
    29161 aatgttatga aacgaagcat ttaaaagact tttggctata tgaattgaca ttacttgtca
    29221 aatatgttaa catataagcg acttgtcgga gggtgtatta aaaagaatat gctggagaag
    29281 agagtgcgtt aactcttttc tccttttttt ataaaattaa gcatttataa atttagtatt
    29341 attacatgat tacactatct caaaaattaa gaaaggagaa aaatatgaaa ttaattttaa
    29401 gttatttaaa gaattataag ttattaatag tattaaacat actggctata tttagttttg
    29461 cattagtcga gttggggatt ccaactataa tagctaaaat aattgacaat ggtattgcaa
    29521 atcagaatat agcttacata aaacaaatgg gaatagtgat tgttattatt tctattatag
    29581 gggttgtagg tagtatattg ttgggttatt gttctgctaa ggtatcaaca agtgtaacca
    29641 gagatataag aaatgacata tttgagaaat cacaagaatt ttcacatact gaatacaata
    29701 aatttggaat ttcttcaatg attacaagaa caacgaatga tgcatttcaa attcaacaat
    29761 ttgttaatat actactaagg actgccttat taactccagt tatgttcatt ataagtataa
    29821 taatgacaat tagaaccagt gtggaacttt ctatagtatt ggctatatca gtacctttta
    29881 taattattgg agtagcaata atagcaaaag tatctcaggc aatatcttca aaacaacaaa
    29941 aaggactaga taaattaaac cttatatcga gagaaaattt aactggaata agagtaatta
    30001 gagcttttgg aaatgatgat tatgaaacag agcgatttga aaaaacaaat acatattatg
    30061 ctaatgtttc taagaaacta tttaagctta tgtcaattac tcaaccagca ttctttttac
    30121 tgttaaatat agctgttttg gctgtatttt ggatttcaag tgagaagata aatattggag
    30181 agcttcaagt tggacagttg gttgcatttt tggaatatct tttccatgcc atgttttcaa
    30241 ttatgctttt ttcaatggta tttattatgt atccaagagc agaggtatct gcaaatagaa
    30301 taaaagaact attaaatgaa gaaccattga taaaaaatcc tgaaaatgga attaaggata
    30361 ctgaaaataa gggtatcata gaatttgata atgtaacttt tacctatcca gatggagaag
    30421 cttctgtact taaggatatt tcatttactg caaaaacagg tgaaacagtc gcttttatag
    30481 gaagtactgg tagtgggaaa agtactctaa ttaacttgat acctagattt tatgatgtaa
    30541 cagaaggtag cattaagata aatggagtag atataagaga atatgattta aaagctttaa
    30601 gaaaaaagat aggattcata cctcaaaaat cactgctttt tactggtagc atagcaaata
    30661 atattagatt tgggaaacat aaggcaggag agtcagaatt agaatattcc gcaaaagttg
    30721 cacaagcata tgagtttata tcaaaaaaac ctagaaagtt tgatgaattg attagtgaag
    30781 gtggagcaaa tgtatcagga ggtcaaaaac aaagactttc tattgcaagg gctattataa
    30841 gaagacctga aatatacatt tttgatgata gtttttctgc acttgacttt aaaactgatg
    30901 caatacttcg tgctaaattg aagaaggaaa caaaagatgc aattgtattg attgttgccc
    30961 agagaattag ttctattatt gatgctgata agattatagt actcaatgaa ggacaagttg
    31021 taggaatggg aactcataaa gaattattaa aaaactgtga aatatactat gaaattgcaa
    31081 catctcagtt aaaaaaggag gaattagaat aatgaaaaag tctaaaggtt ttaagaaaac
    31141 aattggtaga cttcttccat ttgtaaagaa atataaattt tcctttatta tagctattat
    31201 atgtataatt tcagcagcta ctatgaatgc acttgcacct aaaactgaag gattgataat
    31261 aacacaattg actaaagatg taattagtat cgcaaaagga gtaccagggg cttccgtaaa
    31321 ttttgattat gtaacaaaaa tattggtgat tttagcatgt atatattttg ccaatgcaat
    31381 ttttacgtat acttcaagtt ttctacttac gaatgctatt caaaacacaa tgagagattt
    31441 aagaaatgaa gtcgaaaata aaataagaag attgcctatt agttattttg atagtaatag
    31501 ttttggagat gtattaagtc gtatatcaaa tgatgtagat acaatatcaa atgcattgca
    31561 acagagtttt atgcaaattg taaactcaat cttagtcata attttggcac tttcaatgat
    31621 gtttacaatt aatatatata tggctcttat agctcttttt ataattccaa ttagttattt
    31681 tgtatctaaa tttgtagtaa aaaaatctca aagtagattt tcattgcaac aaaatgctct
    31741 tggaaaatta aatgggaaag tacaagaaat gtatactgga tttaatgaaa taaaactata
    31801 tggtaaagaa gaagattcta taaaagaatt taaaaaagta aatcaagaac tttgtgaaaa
    31861 tggatttaag gcacaattta tatcaagtat gatgaatcct atggtttcat tagtaactta
    31921 ctttggaatt gcagctgtgg cagttgtcgg ttctatatat gcagtaagtg gaggaataac
    31981 agtaggaaat ttacaagcat ttgtgcgtta tatatggcag ataaatcagc cactttcaca
    32041 aatgactcag ttatctacag taatacaatc ttcttttgca gctatagaac gtgtatttga
    32101 aattttagac gaagaagagg aaatacctga tgttgaaaat ccagttaaga tagaaaatgt
    32161 aaaaggaaat gttacatttg agcatgttaa ttttggatat ggagaaaatt caactttaat
    32221 agaagattta aatgcagaag taaaaagtgg tcagatggta gctattgttg gacctactgg
    32281 agcaggtaaa acaacattaa taaacttatt gatgaggttt tatgatgtaa agaaaggtgc
    32341 tattaaaatt gatggagtag atataagaga tatgaaacgt aaagatttgc gttcaatgtt
    32401 tggtatggtg cttcaagata catggttatt taatggaacc atttttgaaa atatagaata
    32461 tggtagattt ggggctacta aagaagaaat tatacaagca gcaaaagtag caaatgttca
    32521 tcactttata accactcttc cagatggata caatatgttt ttaaatgaag aagcatctaa
    32581 tatatctcta ggggaaaaac agcttttaac catagctaga gcttttatat cagacccttc
    32641 aatattgata cttgatgaag ctacgagttc tgttgatact aggctggaac ttatgcttca
    32701 aaaagcaatg agaaatctta tgaatggtag aactagtttt gttatagcac atagactttc
    32761 tacaattcgc aatgctgact tgattttagt tatgaataat ggaagtatta tagagcaggg
    32821 gaatcatgat gaattaatgg aaaagggtgg attttatgag aagctttata atagccaatt
    32881 tgcagacaaa gaatcagaat agataacata aacttttgta taaaattttg tatcaaattc
    32941 aaatattaat catataatta aaaagagtat cagtaagttg tttttttata gatttactga
    33001 tactctttga ttttaagact aaaggtattt atgattggta cttataattc acaactagtc
    33061 ttgttacatc tgataaccag aattacagta cttttctttt ttaccttttt gttctctaac
    33121 atataacaat tcgcttaact tatcaatgta ataaccatca tcacttatgc gaattaaact
    33181 agtcagataa tatccatctt tgcttctagg aatcttttta tttattgcta aatcaatagc
    33241 tgtctttgct gcttcaaaat gctttaaatg tgtatgacct tcttcaaaac tctttctagt
    33301 attataaact acataacctt ttttaacttg aaaaactata aattctttct tttcatatat
    33361 tttattaaac attcaatcag cccttctcaa gatagtttta tagcataaca ttagaagctt
    33421 ctaaaaaatt ttacaaagaa cataaatatt ttataaatta taaatctaaa actataattt
    33481 ataaagataa ctttctgatt atttatatga taattcatat agattaaaag taaattatct
    33541 ttaggttaaa caaaagtaaa ataaaagact agttataata tttatatgaa aaagtacaaa
    33601 atatagtata tgaatataga aaatttaaat tatttattta cagtttttat ttccataagt
    33661 atatttacct tcagatacct tgtcatcatg gaaatatatt ctttcacaac ttttatctcc
    33721 ccatagaatg ttttttgtta ttttatggga agtatctcct gtaaatactt cagctacagt
    33781 agtatctgta acatcacagt aaagagaagc aaatttttta aaccaaggat tttcctcaaa
    33841 tcttaaaagc caggaagctc cataggggca atgtaactct tcaaattcag gaacccatcc
    33901 tagaaatgga acatcagttg tgctaataat attgtctgca attaattcat ctccattttc
    33961 atgagctctc tctctcatag caatagctct ttcttcagac ctttcaaata aaacttttct
    34021 taatatttcg ctagttttat cttcaccaaa ctcttcaaat aacactttgg aaaaataaaa
    34081 atataaatca ccaaaatgtt tgcatgccat acgaacttct tcaatagcat aatctttgtc
    34141 aatagtttca ttagttacaa catcttcgtt caaaaaaatc actccttaga ataagtatta
    34201 gttacaatat aattatagaa tattttttat aaattaggaa attaatttga gatagatata
    34261 tttttaataa atgaaagtat aatactaaaa taacttattg ttacaaaaat cttctaaatt
    34321 aatgtattta ggagtttcca tatgaatgag agtagttctg taaatatctt caatacatcc
    34381 atttgcaata ttattaaaaa tatctgtgtg ctcttttaat gttgtttcaa gccttccttc
    34441 atgtgacaat gaatcgagag ctaatttttt tattctgtac atataattgt taaacatttt
    34501 gtcaaattca gtattttgag catatgaaac tatagttgtg tgaaataatg tatcgtattc
    34561 tgcaaatgag tttaaatttt tatcacctaa taaaatatct ttttgtttat ctaaaagatg
    34621 ttttaattta cttatagttt ctactgctct aggttctttg tattgacttg taattaaaaa
    34681 agtacaaaat ccttctattg cagagcgtat ttggaatatc tctacaattt catttgctgt
    34741 aatttgatgg attctaaatc ctttgcttgg aattatatct ataaatccat cttgactaag
    34801 tctttgaaga gcatctctta taggtgttct agagatacct atttcactag caagttttgt
    34861 ttcagaatat atttcatttt ctatcatttc tccagatagt atcaattctt ttaagtagtc
    34921 atatgcttgt tcttgtaatc ttttaaatgt agccatactt aataataccc ccctaaacaa
    34981 tataaaaata caaaaactta tataatttta agcatacaac atagtgaaaa taaggtcaat
    35041 cttttgcaat ttaaattgca gataagatta aaaaatataa aaaattattg aagtgtgaag
    35101 catgattaac attaaaggca gattaattaa aactaattga aattagttaa aatacaaaac
    35161 tgtaaaagta gtatattata tttgaaaaca atagtatgta ttaaaactta tataggatga
    35221 ctaaatataa taaatgactt taaaaattac tatttattgt taaaataaat acttttatat
    35281 tttatattga ccggtatact ggtatgtagt atactctaaa acataaaaaa cgttttcaaa
    35341 aatgtgaaga tatggagggt tataaaatga aattgggatt tattggatta ggaataatgg
    35401 gaaagccaat ggcaaaaaat ttattaaaag atggatttaa tcttttggtg tatgacatta
    35461 ataaatctgc tgtggatgaa cttatatcat gtggagcaaa atacgcaagt gttttagaaa
    35521 tgggtcaaga atgtgatatt gtatttacaa tccttccaaa tggaactatt gtgcaggata
    35581 tattgtttgg gatggatgga ttggcaaaaa ctttaaaaga aggaagtata gtagtcgata
    35641 tgagttcagt tactcctaca gaatcaatat tgtgtgcaaa taaattaaaa gatatgggct
    35701 tagaatttat agactcacca gtaagtggag gggagccaaa agcgattgat ggtacacttg
    35761 cttttatggc tggtggtaag gaagaaattt ataagaaagt agagccattt tttaatataa
    35821 tgggaagctc atctatttta attggagata atggtagtgg aagtgttaca aaacttacaa
    35881 accaagtaat agttaatttg acgatagcag cagtttctga ggcatttgta cttgcagcaa
    35941 aagcaggtgc agacccagaa aaagtatata aggcaataag aggaggccta gctggtagta
    36001 caatattgga tgtgaagata ccaatgatta tgaatcgtga ttttaaacca ggtggaaaaa
    36061 tatctattaa tttgaaagat attaagaatg taatgcaaac agcacacaat ttagatgtac
    36121 cactacctat gacaagtcaa ttattagaaa ttatgcagac attaaaagta catggacatt
    36181 tggaagatga ccatagtgga atagcacagt attttgaaaa gttggcaggt gttgaaataa
    36241 aaaaacacaa taattaatat ttaaatatat caatataatt aaatacatat tattaggatt
    36301 agattatata aatacagcga ggttaagtta tcatagtaaa agaaaaataa aattgataaa
    36361 taataaagta gaatgataaa ggggtaaaga ttatgattac aggatatgga cttttgatag
    36421 catttgttat atctatagga attttacttg tatcgattat taaatttaaa gtaaatccat
    36481 ttttggcatt actgataaca tctataatta caggctttat ggttagaatg cctataaatg
    36541 aaatatcaac cacaatatcg actggatttg gaaatacatt aggtagtata ggtattgtaa
    36601 ttggacttgg aataatattt ggaaatattc tttcagaatc aagagcaaca gaatctattg
    36661 caaaggggtt gttagctaag actggagaaa aaaacagtgc tttagcagta actacagcag
    36721 gttttttaat ttcaattcca gtttttatgg atgcagcttt tgttataatg atgcctataa
    36781 ttaagtatgt ttcgagagta actaagaaat ctctaatggt ttttgtatgt gcattaggag
    36841 ttgggactat agtaggtcat gcacttgtaa tacctacacc aggaccttta gcagttgcag
    36901 caaatgtaaa tgcaaatgta ggtagtttta tactttattc tataattgtt gcttttccag
    36961 cagctcttat aggtggatgg atttatggaa aacgttttga aaaatatcca gcttatgcta
    37021 ttgatgagaa tgatagagaa aaaaatttag agcagggaaa agatagcata aaaattgaag
    37081 atgatgattc ttctaaagca ccaggttttg gaatttctat gttttcttta ttgttcccaa
    37141 tacttcttat acttatatca aatgtattta gtatgttctt agaaaaaggt tctacaatga
    37201 gtggagtact tgcatttata ggagataaaa acatagcaat acttcttgga atattggtgg
    37261 caataggatt tttaaagaaa tacataaata aacctatggg agatgtagtt attgaagctg
    37321 ctgattcagc agggctaata ttattgataa caggttcagg tggtgcgttt ggcagtgtta
    37381 taaatgcaag tggtataggt aacttcttag ttgatactat gtctggtctt agtatatcag
    37441 ttgtagtgct tggattctta ttaagtgcct tgcttagaat ttctcaaggt tctgctactg
    37501 tagccttagt aacaacatca tcaattttag gacctacaat tttggcaact ggaatgtcac
    37561 cagttttagt agggcttgct atatgtgctg gaggagttgg tttctccctt ccaaatgatt
    37621 ctggattctg ggtactttca agatttagtg gattatcagt taaagatact ttaaactctt
    37681 ggacaattgg aggaaccatt gctggagttg tagcatttat aatgatttta ttgcttagtg
    37741 ttataaatgg aattatacca cttccaggtc tataaataac aaactattgt ttatatcata
    37801 taaattacat ttagactata caaattacat aaaggggcgt tttaaatgag agaagtaaaa
    37861 ccaattagca tagatatact taatactttt aaacaagtag atgaggacag attaaataaa
    37921 cttcttgcag atgagcttaa gcatttagat agaaaaattg ttgtattaga tgatgaccca
    37981 acaggtgtac aaacagttca tgatatttct gtttatactg attgggataa ggatagtatg
    38041 gaacaaggtt ttaatgagaa aaatagtatg ttttttatac ttaccaattc aaggggattt
    38101 actgttgctc aaactacaaa agcacataaa gaaatttcaa agaatattgt ggatgtatca
    38161 aagaaagtta ataaggattt tataatcata agtcgtagtg attctactat gagaggacat
    38221 tatcctcttg aaactaattt attaaaaagt gaagtagaga gattatcggg gaagttattt
    38281 cagggtgaaa taataatgcc attttttaaa gaaggtggta ggtttactat agataatgta
    38341 cattatgtta aagaaggaga aatacttgta ccagcaggaa tgactgaatt tgcaaaagat
    38401 aaatcttttg gatataaatc atctgatata ggtgagtggt gtgaagaaaa gacaaatgga
    38461 aagtacaaat ctagtgatat gatatatatt tcgtcaaaag aacttagaga gctaaatatt
    38521 gaagctataa cagaaaaact taaaaaagct gaaggattca ataaaattat tgtaaatgca
    38581 accgattatg tagatgtaaa ggtatttaca attgcgttta tacgagcagt aaatagtggt
    38641 aaggaattta tgtttagaag tgctgctgcc ataacaaaag tacttggcgg agtgagtgac
    38701 aaggaactac ttactaagga tgaattagta tcaaaaggta ataccaatgg aggaattata
    38761 cttgttggtt cacatgtgaa taagactacc caacagttag aagaacttaa aaattgtaaa
    38821 tatccgatag aatttataga atttaatcaa catttagttc ttcaagaaaa tggtctaaaa
    38881 aatgaggtaa aaagagttat aaaagttgta gaggaaaaaa taagcagggg taaaactgtt
    38941 gctgtttata ctagacgaga gcgttttgac ttggatacga atgataaaga taaacagctt
    39001 atgatttctg tagaaatatc agatgcagtt accagtataa ttggtatgct taatgtaaga
    39061 ccaaatttta ttatagctaa gggtggaatt acttctagtg atgttggtac taaagcacta
    39121 agagtaaaaa aagctacagt aatgggacag ataaaacctg gtataccagt atggatgaca
    39181 ggcaaagaaa gcaaatttcc taatatgcca tatataattt tccctggaaa tgtgggagag
    39241 attagcacac ttagagaatc agttgagata ttgatggggt agtatattag tgtattgaag
    39301 tttaaacgct taaatttact gagttaaaat taaaataaac taaaattata aattaagatt
    39361 aaaaataata gtatattaat tagggtttag agtattttac a
//
LOCUS       DAKBNN010000012        38021 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000012 DAKBNN010000000
VERSION     DAKBNN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.012"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            120..2033
                     /locus_tag="OUB02_000649"
     CDS             120..2033
                     /locus_tag="OUB02_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8884446.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGIFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSNKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            2304..3278
                     /locus_tag="OUB02_000650"
     CDS             2304..3278
                     /locus_tag="OUB02_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCT8884447.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            3299..3574
                     /locus_tag="OUB02_000651"
     CDS             3299..3574
                     /locus_tag="OUB02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCT8884448.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            3587..4750
                     /locus_tag="OUB02_000652"
     CDS             3587..4750
                     /locus_tag="OUB02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCT8884449.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            5088..7367
                     /locus_tag="OUB02_000653"
     CDS             5088..7367
                     /locus_tag="OUB02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8884450.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(7462..8802)
                     /locus_tag="OUB02_000654"
     CDS             complement(7462..8802)
                     /locus_tag="OUB02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8884451.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFYLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(8814..9596)
                     /locus_tag="OUB02_000655"
     CDS             complement(8814..9596)
                     /locus_tag="OUB02_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884452.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(9611..10633)
                     /locus_tag="OUB02_000656"
     CDS             complement(9611..10633)
                     /locus_tag="OUB02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCT8884453.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGKGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(10639..11652)
                     /locus_tag="OUB02_000657"
     CDS             complement(10639..11652)
                     /locus_tag="OUB02_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCT8884454.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(11780..12718)
                     /locus_tag="OUB02_000658"
     CDS             complement(11780..12718)
                     /locus_tag="OUB02_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8884455.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAVPFFSQSYNPIGKELLLESVKNELTK"
     gene            12988..13452
                     /locus_tag="OUB02_000659"
     CDS             12988..13452
                     /locus_tag="OUB02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884456.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(13564..13881)
                     /locus_tag="OUB02_000660"
     CDS             complement(13564..13881)
                     /locus_tag="OUB02_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT8884457.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(13894..14625)
                     /gene="chbG"
                     /locus_tag="OUB02_000661"
     CDS             complement(13894..14625)
                     /gene="chbG"
                     /locus_tag="OUB02_000661"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCT8884458.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(14651..15961)
                     /locus_tag="OUB02_000662"
     CDS             complement(14651..15961)
                     /locus_tag="OUB02_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCT8884459.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCTNPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(16038..17351)
                     /locus_tag="OUB02_000663"
     CDS             complement(16038..17351)
                     /locus_tag="OUB02_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8884460.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(17373..17684)
                     /locus_tag="OUB02_000664"
     CDS             complement(17373..17684)
                     /locus_tag="OUB02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884461.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(17884..18588)
                     /locus_tag="OUB02_000665"
     CDS             complement(17884..18588)
                     /locus_tag="OUB02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8884462.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCEDSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(18679..20040)
                     /locus_tag="OUB02_000666"
     CDS             complement(18679..20040)
                     /locus_tag="OUB02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8884463.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            20539..21693
                     /locus_tag="OUB02_000667"
     CDS             20539..21693
                     /locus_tag="OUB02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8884464.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(21775..23493)
                     /locus_tag="OUB02_000668"
     CDS             complement(21775..23493)
                     /locus_tag="OUB02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HCT8884465.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(23498..24487)
                     /locus_tag="OUB02_000669"
     CDS             complement(23498..24487)
                     /locus_tag="OUB02_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8884466.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(24583..25632)
                     /locus_tag="OUB02_000670"
     CDS             complement(24583..25632)
                     /locus_tag="OUB02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8884467.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(25909..26577)
                     /locus_tag="OUB02_000671"
     CDS             complement(25909..26577)
                     /locus_tag="OUB02_000671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCT8884468.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(26583..27956)
                     /locus_tag="OUB02_000672"
     CDS             complement(26583..27956)
                     /locus_tag="OUB02_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCT8884469.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(27953..29731)
                     /locus_tag="OUB02_000673"
     CDS             complement(27953..29731)
                     /locus_tag="OUB02_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCT8884470.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(29848..30171)
                     /locus_tag="OUB02_000674"
     CDS             complement(29848..30171)
                     /locus_tag="OUB02_000674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCT8884471.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(30158..31135)
                     /locus_tag="OUB02_000675"
     CDS             complement(30158..31135)
                     /locus_tag="OUB02_000675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HCT8884472.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(31150..31713)
                     /locus_tag="OUB02_000676"
     CDS             complement(31150..31713)
                     /locus_tag="OUB02_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HCT8884473.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(31765..32256)
                     /locus_tag="OUB02_000677"
     CDS             complement(31765..32256)
                     /locus_tag="OUB02_000677"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCT8884474.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(32259..34184)
                     /locus_tag="OUB02_000678"
     CDS             complement(32259..34184)
                     /locus_tag="OUB02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCT8884475.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(34186..34497)
                     /locus_tag="OUB02_000679"
     CDS             complement(34186..34497)
                     /locus_tag="OUB02_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884476.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(35127..35402)
                     /locus_tag="OUB02_000680"
     CDS             complement(35127..35402)
                     /locus_tag="OUB02_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HCT8884477.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(35517..36926)
                     /locus_tag="OUB02_000681"
     CDS             complement(35517..36926)
                     /locus_tag="OUB02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCT8884478.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(37297..37959)
                     /locus_tag="OUB02_000682"
     CDS             complement(37297..37959)
                     /locus_tag="OUB02_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCT8884479.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
BASE COUNT        12723 a         6561 c         4830 g        13907 t
ORIGIN      
        1 ttgatacagt cttttataat gttcctttta aaaatacttt atttataatt aaatataaag
       61 taaaatgtta aaatataact aataaaattt agtcataaga taatatttaa gtttatttga
      121 tgaaatatgg aggaattatt atgaaggaaa tactcgttat tgctcccact aaaggaacct
      181 atgaaaaatc aattcacata gttaaaaaaa acaaatatac aaatattgat gttgtatttg
      241 gcaatctaaa agaaggtata cctcttgctg aaaaaagtat taatcatggt actagaatta
      301 tcataagccg aggtggaact tacaatatgt taaaagctac ttacaatata cctatagttg
      361 aaattaaagt agatgcttat gatattataa aaagttacaa agaggtaaaa aattccaacg
      421 aaccatttgg aataattgga tttaataatg tcatctacgg tttcgatatt atagaagaaa
      481 tattgaataa gaaaataact atgattgaaa ttgaaaaaga agaagaaatt tatgatgcta
      541 tagaaaagta cagaaagaaa ggtataaaca catatatagg agataccact gttgcacata
      601 ttgttaagag acttaattgt aagggaatat taatcgaatc tagagaagaa aacatactta
      661 gagccattca acaagcagaa caaatactcg aagcaaccaa ggatgaacaa aaaagaagat
      721 tacagataga agctatgaca gattttgtac atgatggaat aataacagtt gataaaaatt
      781 ttaaaataac attatttaat aagagtgctg aaaaaatatt tggtataaaa aaagaaaatg
      841 cactttatca taatgttgta gatgttatcc caaacacagt tcttccacaa gtagttgaga
      901 ctatggaacc tcaaattggt gaaatacaaa atatagggag tacaaaaatt attacaaata
      961 gagttccaat cattgttgat ggtcagattc aaggagctgt ttcaacattt caagatagca
     1021 aggagataag ttcttttgaa catacaataa gaaggagctt attaagcaaa ggtctatctg
     1081 ctaaatacac ttttgatgat ataattcatg ctagtgaaaa gacaaaagat tgtatagaac
     1141 ttgcaaaaaa atatgcttgt tatgatactc ctatgattat tactggagaa tctggagttg
     1201 gtaaagaact attttgtcaa agtatacata actatagtaa gcgtaaaaat gcaccttttg
     1261 ttgctgtaaa ttgtgctgcc atacctccat ctctaataga gagtgagttt tttggttatg
     1321 aggaaggttc atttacaggt gctagaaaaa aaggtaaagc aggtatattc gaactagcac
     1381 atgaaggtac aatcttccta gatgaaataa gtgaaatccc tcttgaactt caaggtcgat
     1441 tattgagagt attgcaggaa aaacaagtta tgagaattgg tggtgataaa gttattccta
     1501 tagatgtaaa aattatctgt gcttcaaata aagacttaaa aaatatgatg cgcaatggtt
     1561 tatttagaca agatctttat tttagaataa atatactaac attatatctt ccttcattaa
     1621 aagaaagaaa agaagatatt ataaaattat cagaattttt tatacataaa tattcaaaca
     1681 aatacaataa aacgccctta attatcactc caaatattaa aaaggcactt cttgaacgag
     1741 aatatgaagg taatataagg gaattggaag gtatgatgga aagggctgtt attgtggaat
     1801 catttgactc gattttatta gaaaaggtta gcaatgaata tacaaataat atagttgata
     1861 aagataatag tgattttgaa tgcagtaatt tagatttaaa aactttagaa aaaaaatata
     1921 tctataaaat ttataacgaa aataatagaa atactacaaa aacctgtgaa attctaaaga
     1981 taactcgctc cactctatgg cgtaagttaa aagaaataga agatgatgtt taaaaataat
     2041 acatatctat atatatcaaa acgttttatg tttaatttta atacataaga cgtttatttg
     2101 ttttcaataa aaaatacaat tatatgataa aaagaatctt tgtaagtgaa attctcgtca
     2161 atcgtacttt taattataag taatctacag catttcaatc gattattaaa aataatcatg
     2221 ataactaaag atacaaactc tatatattgg catattaatt gcttttataa tacctatata
     2281 aaaatgaaag ggagtgatac tagatgcaca tcttaaaatc agtgaaaaag ataccaggag
     2341 gacttatggt ctgtcccttg ttattgggag cactatttaa tacagtatgt ccacaagctc
     2401 ttgaaattgg aggattcaca acatccttat ttaaaactgg tgcaatgagt atacttgctc
     2461 tattttgtct atgcaatgga gctcaaatca atatcagaca agctggtaaa ccattgacta
     2521 aaggtatagt acttacagca acaaaattta taattggtgc agtattaggt attattgtta
     2581 gtaaatttat tggacctaaa ggtataattg gtattacacc tcttgccata gttgctacca
     2641 tgactaattc aaatggaggt ctatttgctg ctcttgcagg tgaatatgga gactccacag
     2701 atgttggagc aatatctatc ttatctctaa atgatggtcc tttttttaca atgcttgctt
     2761 ttggagtaac tggacttgca aatgtaccta tagtagcact atttgcagca cttataccaa
     2821 tactacttgg atttatactt ggtaaccttg atgaagatat acgagaattt cttgcacctg
     2881 gtacaacatt attaattcct ttcttcgctt ttcctttagg agcagcatta aactttaatc
     2941 aaattataac tgctggacta cctggagttg tacttggttt aggagttact ataataacag
     3001 gtatgggtgg gtactttgta atgaaactta tacgtgcaga acatccagca gtaggtgcag
     3061 ctacaggtac aacagccgga aatgcagctg gtactcctga agctttagca gctatagacc
     3121 ctactctagc tacaatagca gctgtttcaa cagtacaggt tgctgctgct attattgtta
     3181 cagcaatatg ttgtccaatg ttagtttcct atctagataa aagagaaaaa aagaaaaaat
     3241 ctatttttaa aacaaataaa aatattgaag taaattaata cttaaggagg taaaattaat
     3301 gaatgcaatt attcatgcaa atgaaaaaga taatctagtt acatgtgtca gacctttagt
     3361 taaaggtgaa aaagtttcaa ttgatggaaa gtcttacata gtaaatgata atatacctgt
     3421 ctttcataag atggctacag aagatgtaaa aaaaggtgat gttgtataca aatacggtga
     3481 aatcatagga attgcaacag ttgacataaa aactggtgac tatgttcatg tacataacat
     3541 tgaaagtaca cgtggtcgtg gagataaaaa ataggaggaa gttaaaatga aattaatggg
     3601 ttatttaaga gaaaatggtc agtttggtat tcgtaatcat gttttaatta ttccaacatc
     3661 tgtatgttca agtgagacag ctacaagaat agcatcactt gtgcctggag caatagcaat
     3721 ccctcatcaa catggatgtt gtcaaattgg ttctgatata gagcttacag caaagacatt
     3781 aataggattt ggaaaaaatc ctaatgttgc cgcagtttta gtagttggtc ttggatgtga
     3841 tggtattcaa gcaaaagaat tagcatctga aatagctaca acaggaaaaa aggtagatta
     3901 cgttgttata caggaatgtg gcggaacatt aaaaacagtt tctaagggag ctgaaatagt
     3961 aagtaaaatg gctcgtgaag tgtcaaaaga agtaagagtt gaatttggta tgagtgaaat
     4021 tacattagca cttgaatgtg gtggttctga cccaacaagt ggaatagctt caaacccttc
     4081 aataggcgtt gcatcaaacc ttcttgtaga tgaaggtgga agtagcatac taagtgaaac
     4141 tacagaagta attggtgcag aacatttgtt ggctacccgt tttgaagatg aagaaatgaa
     4201 agataagttt cttaaatttg taagtgatgt agaaaaaaga gcaattgcta tgggagaaga
     4261 tttacgtagt ggtcaaccta ctccaggcaa taaaactggt ggactttcaa caattgaaga
     4321 aaagtcactt gggtgtatgt ataaggcagg caataagcca ttcaaaggtg ctcttgaata
     4381 tgctgatatt gtacctcctg ataaaaaagg attatatttt atggatactc caggtcaaga
     4441 tatagattct atcactggta tggtagcagg tggtgcacaa attgtaatct tttcaactgg
     4501 tagaggtact ccaacaggaa gccctatttc accagttatt aaaatcactg ggaatagtga
     4561 tacatacaac aaaatgccag acaatatcga tataaatgca ggtagaatta taacagatgg
     4621 tgctaaaata gcagatatag gtcaagaaat atttaatgaa ataatagaag tctgtaatgg
     4681 taaacatacc aaagctgaaa gtcttggtca tagagaattt ggcatttaca gaatttcatc
     4741 tacattttag attgtaacaa aatatctttt ataaatataa tacttatgta gatacataag
     4801 ctaaaagttc tttctaatac tataaaaatt ttctatactg aaaaggaact gtatcaaaat
     4861 tattttgaaa acagttcctt tttttaacct cttttactca aaatattata aaataagatt
     4921 taaacaataa acgtatcttg attaatctct aagaagacat aaataaagct aaatgtaaag
     4981 tgggataaat aaaatttaat atctttgtat gaaaagatat ttggtataat atgtctgaga
     5041 gaattcttat ttgtaaacaa ataaaggatg gaggatgaaa caaaagaatg aactttgaaa
     5101 acacaaaaaa taaaaaaata atagctactt tactattgac actcctttta atcgtgtcat
     5161 taacatgtgt cacttttatt atgaatctaa ataaagtaac acataatgaa acagaacgat
     5221 atcttgtaga agtttcacaa ggtatttcca ctacaattaa tgtgaaatgt aaaagtatat
     5281 ttaggacact atattctatg ggagatactt attttgagat taatgatagt gaaaaagaag
     5341 tcaatagata cttatcaaaa aaattaaaat tatttgaatt tgattggtta ggtattatag
     5401 attctaatgg aaattttgta agtgccctta aagatacagg taatgttaaa ggacaagact
     5461 tttatagaaa agcccttcaa ggcaaagaaa caattactcc tgttaaatca caatctggta
     5521 catatggaat tttatattct gtaccactat acaatggcga taaaattgtt ggagctgttg
     5581 ctgcttggaa tactattgat actatgagaa atttattaag tgtagaaagc tttcatggtg
     5641 aaggattttc ccaaattatc gaccaagatg gaaattttat tgtaagttca gcaaacaaaa
     5701 atgctccaaa aaatgtggta aatttttttg acatgattaa acaccaagga aaacaggttg
     5761 ataaaaataa acttgaagaa atgaaaataa acttaaaaaa gaacaaaact ggttctatgt
     5821 attatacatt aaatgatggt atttctaagg caatgcacta tcttccatta caagaagaaa
     5881 acttatacct cctttcaatt gttccaacaa aaacagcaag tgcacagttt gatttacttc
     5941 taaaaaaagc aatcttcatc aatattttaa ttgttatact atttttattg cttattatat
     6001 tgattatgtt tataaatcaa aagaacaggg cacaacttat aaaaatcgcc tatgtagact
     6061 ctgtaactaa aggttacagt aaagcacgtt ttgatattga agctaaaaaa attatacagt
     6121 catcttcatc agaaagctat tcattggtat ctataaatat tcagaaattc aaacttatta
     6181 atgattcttt tggtagtgaa gcaggagata aaacattaaa gtacatacat gatgtcatta
     6241 gtaattatct agaaccagga gaattgcttt gtcgagtttc taatgaccag tttaatgtac
     6301 ttattaaaac tattcctcaa aaagaaattc ttaagtacat agaaaatatc accaatgata
     6361 ttaatagttt caataaaaat gaagaatgta aatattttat agctctatct ataggtgtat
     6421 ataaaattga tgacccaaat ctacctttag tttcaattca agaccgtgca aatgttgcac
     6481 gaaaaaataa taaaggaaac attggaaatc atccacttta tacatgtgtt ttttatagtg
     6541 atttagagcg tttaaaaatg attaaagaaa aagaaatgga aaatagaatg gaggaagcat
     6601 taaataataa cgaatttata gtatatcttc agcctaaaat cagcctaaaa gataatacta
     6661 ttgtaggtgc agaagcatta gtaagatggc aagacccaaa taaaggcctt ataccaccag
     6721 acgagtttat tcctttcttt gaaaaaaatg gttttattac aaaactcgac cactatgttt
     6781 ttgaaaaagt atgtagtatg ataagaaaat ggatagataa tggagaaact ccaatacctg
     6841 tttcagttaa tttttcacgt gctaatttaa ataatataga ttttttagaa aaatacagaa
     6901 tgcttcgtaa taaatacaac attcctgcaa agctcttaga aattgaatta actgaaacac
     6961 ttgtatttga aaatctccaa aaattaatta atataataga ccaaatccat gaagaaggtt
     7021 ttcaatgttc actagatgac tttggaagtg gatactcttc actcaatatg ttaaaagaaa
     7081 taaaagttga cacattaaaa cttgaccgtg ctttttttag ctcacctaat gcagataata
     7141 ttgctgaaaa tcatgtaata gaatctatag ttaatttggc aaaaaaatta aatatgaata
     7201 gtatatcaga aggtgttgaa acaatcttgc aaatggatta tcttaaaaaa attaattgtg
     7261 atatgataca aggatatgtt ttttcaaaac ctctacctca agaaatattt gagaaaatga
     7321 cttttggaaa aaaactagaa gaaaccagta tggatagttc cgtttaaata aaataaaaaa
     7381 cttgtctcta taaaataatt ttaatcattt tataaagaca agtcttttta ttttccaatt
     7441 taattttatt ttctaaattt cttacactcg gcttgtttta aattcccttt ttaagaataa
     7501 taatacgatt atcatagcaa taaaatctgc aactggctgt gccaaccaaa caccatctaa
     7561 attaaattga attggaagta caatgattaa tggtataaat aaaataaatt gtcttaaaag
     7621 agttaaaaac attgaatgtt taactttact tatacactga aaatacacag gtcctacaat
     7681 agaaatcccc attataggca atgtaattag atttaggctt atacctctta ctgctatatc
     7741 catcaaatct gaatctttat taaatatacc tacaaatact tctgggaaaa cttgtactac
     7801 taaaaatcca aatgacaata tgacaactga cactatgata gataatatta aagttttctt
     7861 tgcacgctca tattgttttg caccataatt gtatgatatt atagtttgca taccttgatt
     7921 caatccaaac acaggcatca taaacatcaa taaaatagat gtaagtgctg tcatagctcc
     7981 aatagctaaa tctccaccat aaacctttag agaattattt gtaaacatgt gagttatact
     8041 tatagctact tccatagcaa aaggtgtcat tcctattgca aataccttct ttactatatc
     8101 tttattcaat tttaagttac atttttttag ttttaaattg gacttccctc ttataaaata
     8161 atgtattacc caaaagaata ctactatttg acagagtact gttgctatag ctgctccttt
     8221 tatacctaaa ttaaatacaa atataaatat agggtccaat atcaggttta atacacatcc
     8281 tacaatcatt gtccttgctg ctaattttgg attaccttct gccctaattg cattattaag
     8341 ataaaatgca actaaattaa atatacttcc aaccaatatg acactcatat agtcttttgc
     8401 ataaaatatt gaatctttac ttgcacccaa aataaaaagt atatcttcta agaaaactaa
     8461 tcctaatata gttataatta aagagataat tatagataat gtcaaagcat tacctaatat
     8521 cttctctgct tcttctctat ttctttcacc taactttata gacaatcttg tgctagcacc
     8581 tactgatacc aacattccaa aagctattat tagagtaaat actggcatag ttacaccaag
     8641 acctgcaata gctatagagc caataccttc catagaacca ataaaagctc tatctactac
     8701 attataaagt gaagttacca tcattgctat tattgcaggt atcgaatacc taaataataa
     8761 aatccatatt ttttcatgtc ttaatgcttc ttgattctcc atgttgtcac catttacacc
     8821 atgtcataag ttatacaaat tggtttattt gttcttgggt ctgttgctat ttctgcatca
     8881 atattaaata attcttttag attatccttc gtcataacat catatgcatc accttcacaa
     8941 actattttac ctttcttaat acctatcatg tggtcagcaa atcttgctgc attattaagt
     9001 tcatgtatta ccatgacaat agttcttcct tgttttttat ttagttcatc taataatttt
     9061 agtatctcca attgatgtgc caaatctaaa tatgttgtag gctcatccag taataatata
     9121 tcagtttctt gtgccaaagc catagctatc catgcccttt gtctttgtcc tcctgataaa
     9181 tcttccatat ttctatcctt aaattctgca attccagtaa cttctaaact ccaatttaca
     9241 atctctctgt cttctttact agaattacca aatccacttt tatgaggaaa tcttccataa
     9301 gaaattaatt ctgaaacggt aagaccacta ggagcttgag ggctttgtgg aagtacagcc
     9361 atatcctttg ctatttcttt agacttcttt ttgtagatgt cctctccatt tatatatatt
     9421 ttcccactat tagggctaat aattctacct atagttttaa gtattgtcga ttttccacaa
     9481 ccattagccc ctatgatggt agttattttt ccttttggta tatttaaatt taaatttttt
     9541 ataatatctg tgtttccata tgatacattt agattcttag tatttataca gttcataaca
     9601 acaccctttc ctaatcattt accaacatta aataaataaa gtacggcact cctattatag
     9661 aaactactat acctactggt atttctatag gtgctagaag atttcttgat atagtatctg
     9721 ctattaaaag taataatgtt cctatcaatg ctgatactgg tattaatctt ttatgctttg
     9781 gtcctattat ttttctacct atatgtggag ctattaaacc taaaaatgaa atactacctg
     9841 ctactgctgt cgatacacca gataaaatta ctgcatatat tataagtctt cttctttgtt
     9901 tttctacatc tacaccaaga cttatagata cttcatcacc caaatttaaa acatctatat
     9961 atttagattt ataaatagtt atacccataa aaatcactat aaatggaagc acagctatta
    10021 catatttcca actggttccc caaatgcttc ctgatgtcca tgccataacc ctattaaatt
    10081 cttgtgttgt aaatttcaat tggaatatcg tcaaaattga agtaaaagct atgtttacac
    10141 ctataccaat aagtagtaat cttgacgaat ttataccacc tttccatgca agagaatata
    10201 taagaaatgc tccaaataaa gctcctataa gtgcaacaat aggcattgta aatacagtca
    10261 agttactcat accttcatat acattaccat tcataagata tatataaact actacaaaca
    10321 atgcagcacc tgaatttatt cccaaaattc cagaatcagc taaatcattc tttgtaactc
    10381 cttgaagtat tacacctgct gttgataatg cagaaccaac caaaagtgct attactattc
    10441 ttggtaatct taatttaaat attgcaattt catgattacg ctgacccttc cctaaaaaag
    10501 tctttataac atccattggt tctatagaaa atgagcccat atttaaactc aataaaaaag
    10561 tgataaaaat aagcaccatt aaaattgata taacaagtaa tgttttagaa tttattttta
    10621 taatattttt catagtacct atttctcccc ctttctaaca aggtatataa atattggcac
    10681 tcctataagt gctgttatag aacctactgg tgtctcatat ggaggattta tcatcctact
    10741 taatatatct gtatatacaa gcaatacagc tcctaatact aaagagctcg gtattatata
    10801 cttataatct gaacctacta ttcctttagc tatttgagga actataagac ctataaaaat
    10861 aatattacca gacactgaca cagatgctcc agtcattaat attacaagta ctatacatat
    10921 aaatctaaca aagttagttt tttgtcctaa tcctattgcc acttcttctc caagacttaa
    10981 tatagttatt cttggagcca taataatagc taaaataaat ccaataccac cagctacaaa
    11041 tagcaatttt ataccttccc acttcactcc tacaagccct cctgatatcc agaaacttaa
    11101 ttgttgagat aggttaaaat acatagatat tgctgatgcc aaagaaatta gcaatgtacc
    11161 aagagcgact cctgcaagag ctaattttac attattaact cttcctcttg atttagaact
    11221 tataaaatat ataaataatc cacttatact tgctccaata aatgcaaaca tcattacact
    11281 aaaacttcca tgtataagtt gaggaagttt tttttgaagt accagtgata tcgaaattgc
    11341 gaatgtagct ccttgtgtaa ttcctataac agatggttca gctataggat ttctcgttat
    11401 tccttgcatt attgccccag aaactgctaa aaaccctcca acaagtgcag ctgctattgc
    11461 ccttggaagt ctaacatcct ttactattct catatttatt ccatcttcca tttttatcaa
    11521 actatctata acagttgata gattcatatc ttttgcccca atagttatgg ataatacaat
    11581 tccaaataca agtacaacta tacttactaa tatcaacaaa taagttatat tttttttact
    11641 cagcttagtc aattatccta cctccttttc ccttttaatc gtcagtataa tatcatctat
    11701 taagtatttt aattttaaga tttattaaaa ataggtgtga aaaatgaata tcattatctc
    11761 acacctacta agttaagaat tactttgtta attcattttt aactgattct aataataact
    11821 cttttccaat tggattgtat gattgactga aaaatggaac agcatcaagt attgttacat
    11881 taccatcttt tactgctctt atttgagccc agatagcact attttctaaa tcttttttat
    11941 ctgcttctgt agcaattaca actatatggt ctgcatctat atctgttaaa ccttccatac
    12001 ttaccattgg tagtgttatt ccatcctgtt ttggcatatt cttaggtcta gctaatttca
    12061 tatcatcatt gattagagta cctattccac catcactaaa taccataaat tgacctgaac
    12121 ttgccaacac tggtaaataa gttttttctc cattcttttc tataacttcc ttacctaact
    12181 ttgtagcttt ttcatcatac ttctgtagcc aacttttagc ttcttcttct ttgtcaaata
    12241 actttgaaac atcagttaac ttgcttctcc agtcatttgc ataatctttc atcataacta
    12301 caggagctat ttcttttaat tggtcatata ttttttcttg tctttgactc attattatta
    12361 aatcaggatt tacttctaat atagcttcca tatccatagt atccatcatt gagtgcccaa
    12421 ctatctttac acctttaagc tcttctctta tgtaacttgg taatttatca gtttcatatg
    12481 agtctacatt tgctgttgca acaggcttat aacctgctaa taatagttct tcactacttc
    12541 ctgatatatc aactattttt ttaggatttg atggtatctt aacctctcct ttaacagatt
    12601 gtacaactct tgtctctgat ttatctttat cctctgtatt cttatctgaa catgctgtaa
    12661 caagtactaa tgtagttata atagatataa atattgcaag tgattttatt tttttcatga
    12721 ttacttctcc tacttgttta ttatacttac atgatattga ttatcataag cacatattta
    12781 ttatagcatt tttctatttt tagtcaatat tattttgtaa aaggataaaa attcctaatt
    12841 aatcttaatt atatatagct tagataaggt tttcttatat taaacagtat ttttttaaag
    12901 aattggacac aaatacaatg acttttcagt taaacaatga taaaatgcaa tttatagata
    12961 atgaatttag taaggaggat tcaaaacatg gtacatttag tatattgtga taatgcagga
    13021 aaaaaaggag aaaaggtctt agataaaatc ttggctggaa caaaaacaat ggttattaga
    13081 ggtgctgctg gtagaaaaat accacatagt agagtatttg aagatgagtt actttatttt
    13141 atggaaaaag gaactgcaaa aatatctgca aaagccactg ttaaaaaggt agaaaactat
    13201 gttaaattaa cagaagatga aatttctaaa attttaaatg aaaatcaacc aaaattaaat
    13261 ttatctgaaa agcaaaaagt tcgttggcat aaaaaatgtt tatgcttagt tgaatttgaa
    13321 aatgttgaag aaataacacc tcttgatttt gaccatcaag gaaatatgga tgattggctt
    13381 attgttgaaa aaattgaaga tgtagttgtt ggtactagta taccttacaa ttatgagaag
    13441 tctaaattct aaagtatcaa taacataata ctatattgct aaactctact taaataaaaa
    13501 aagagtgcat ttaataatta taaaaattat ttttatacac tcttttttaa taataaattt
    13561 aaattactta ctttctaatc tttcatatac atctataaat tcttctgcta attgttgaaa
    13621 attcatagct gtcattaaat ggtcttgtgc atgaataagt ataacactta tttcagtttt
    13681 attaccatta gcttcatttt gtatcaattc tgtttggaat ttatgagctt tatgaattga
    13741 ttctttcgac ttttcaatac attctctagc tttatctata ttcccgctct ttgcttcttt
    13801 tattgcttca aatgctagcc ctttcgcatc acctgcaaat ccaattattc caaaagatat
    13861 ctctatcatc ttctcatcca tttattcgac ctcctaaata tctctatagc ttgatatgac
    13921 taatccattc ttttctaaaa attctttgac ttttttagat gtaagtatct tgtgctcttt
    13981 cgttctatca atagcataag atgtagaatt taaaatataa tcatctaaaa aagctgggtg
    14041 agacataata tccactacat catacttcat tatttcgtct atatttttta taaaatattc
    14101 ttcacttaca ttttccttat aaaaactatc tattaaagga actactttag aatactctaa
    14161 attatattca aatccacctc ttatcggaag attatacttg gttacaatct tctctattac
    14221 tggctttaaa cttactaaag tatggatatg atggtgacta tctaaatgag atatatctat
    14281 tccaagccct tttaccctat ctatttgagc acaaagttct ctataaatct catcacaatc
    14341 cattttttca ataatttcgt ctgtaatcct cctaataaaa aaaccgtctt tatcaacaat
    14401 agtttttaag ttagataata atggtctacc acaacttaaa gtcatatgaa ctccacaatt
    14461 taaagtttta ttttctttaa gtaagcctac tccatgttct accccaggca tatttgccat
    14521 catagaagta gacctaacta taccattatt atgagctgaa attattccat agttcacagc
    14581 ttcacaatat ccaaaatcat ctgcatttat tataatttta gtcataccat aacctacttt
    14641 ctatagacaa ctatctatta aatctaggaa gataatcttt atgagcttcc aacatttcgt
    14701 ctaatatagt ttttgctaag tcatcagatg gaatcaatgg gtttgtacaa agtgccaaaa
    14761 gagcagattc ataatcgcca tatacagctg ctttagctgc caatacttca aatgatttga
    14821 tttgacttac taatccatgc acagaatctg gtaaatatcc tatacttatt ggttttggac
    14881 cattttttgt tattacacta cttacttcta ctgcttgttc atctttaaaa tctcttatag
    14941 aacccttgtt taatgtatta actacttgga tatctttttt gtcattgtat atagaactta
    15001 ttaaattaca agcagcatca ctatagtaag cccctcctct tttttcaagt tgaggtggtt
    15061 ttacatctaa tttttcatcc ttatataatt caaataattg ttcttccaat tctttaacta
    15121 cttgacctct agcttttccc tctttaaact ctctcaactc atcctcaagc atttctttag
    15181 ttttatagta gtatctatga tatggacaag gtattgcacc caaagatttt acaaattcag
    15241 cattaaaatc aattgccttt atattttgca tacttatatc tgctttgaca aatttatcaa
    15301 ttgcttcttt tgtaacatct tctccatcta aagatacatt tagaccataa accatatgat
    15361 taagacctgc aaaatccata cttattctgt cactttctac attaaatagt ttggcaacat
    15421 cattttttaa atgaattggc acgttgcata gacctatata tcttctaaaa ttagtatatt
    15481 tgaatacagc ctctgttatt attcctgttg gatttgtaaa attcaccatc catgcatttg
    15541 gacataattc ttccacatct tttattatat caaatataac tggtattgtg cgaagggctt
    15601 taaataaacc tccagcacca ttagtttcct gccccataac tccatgagat aaaggaatag
    15661 tttcatcttt tattctcgca tctaaaagac ctactctaag ttgtgttgtc acaaagtctg
    15721 catctcttag agcttctctt ctatctaaag taagatttat tttcatatct actccagctt
    15781 tttttaccat cctcttagct agatttccaa ctatctctaa tttttctttt ccagcttcta
    15841 tatctacaag ccataattcc tttacaggta actcttcata tctatttata aaaccctcta
    15901 caagctcagg agtataactt gaacctcctc caattgtaac tatttttagt ccctttttca
    15961 tctcattata ccatccttac ctataattta atatactcta caaaacccta tacatttatt
    16021 taccgaaata aaactaccta gttgttaatt ttcaatttat tagcttccat tttttcagac
    16081 gctatgacaa atggaatata tattaatatt gataatatta aattaaatag tgctaaagct
    16141 ccaccagtcc agtcacctgt agccattgct ccacctaata taggaggtgt tacccaaggt
    16201 attttaggta aaattactgg tgctactaat ccaaaatcta ttgctaagta tgatacagca
    16261 ctacatatta ctggtgcaag tacaaatgga atcataaata taggatttaa aactattgga
    16321 agtccaaata tcaatggttc tcctatgtta aatattgcag gtggaccacc taatactatc
    16381 atctctttac gtcttcttcc agctattaat aaagctacta ttaatcccaa taccattcca
    16441 gaaccaccta aatacacaaa tgcatcaaag aatgcacctg caagtacatg gtatccttcc
    16501 gttgctcctg cttgagccaa ggcagcattt tctccaccta atttcataag tatagtttct
    16561 gtaaaaggaa gtgctatatt tgcaccatga agtccaacag tccaaagtat atgaattata
    16621 aatgcaataa gcattgtaga acctagacta tcagctacat ttgatagtgg tgctcctaaa
    16681 taagtattta aaatatctgt taagaaactt ccattagaaa gcatttttat aaatattcct
    16741 attacagtaa atatcattat agttaacatt cctggtatta actttgcaaa tgatcttgaa
    16801 actgctggtg gaactccatc tggcattttt atagttaatt tctttacctt agataatctt
    16861 acaaatatct cagttgataa taatcctatt aatatagaag agaataaagc tgctgtacct
    16921 aagtaggttt gttgtatcat tccccatcct tcaactactg tagttcctcc ctcaggaact
    16981 attttaccaa tttgtggagc cattataaaa aagatagata aagctataag cccagattgt
    17041 aacccatcac tctcataaga ttttgctaga aaataagcaa cacctattac taagaatact
    17101 gccattgtac ctattgctcc ccaccaaagg tcgcctccaa atgtggtcca gttttctcca
    17161 aatatacttg ccatcaaatt tttgtatgct tccaatggta agttatttat caatgttccc
    17221 aatgcaccta ccatagttat aggtatcatt actataaatg catctcttac tgctactaag
    17281 tgcctttgag ctcctatttt agcagctaca ggcactatat atttatccat aaatgacatg
    17341 aatttttcca ttttacatcc tccagtacaa ctttattttg aactatttaa ctctacagct
    17401 ctatctaaca cagcttttcc attacatctt ccataatcca tcatatttat aacttctact
    17461 ggaatattat atggttttgc catttcagtt gcttttccta atacatatct aacttgaggc
    17521 cctaaaagta agacatcaca attttctatt tcatttttta agttcacttc tgatatagcc
    17581 catatttttg cttctatacc gttttccttt gctgcatcct ccatttttgt aactaataaa
    17641 ctagttgaca ttcctgctga acaagctaac attattttta tcattttttc ccctccaaat
    17701 tagattcatt aaatattttt taagtcataa gttttttaaa ctatacattt aaaatatgct
    17761 tttctattaa ttttttctta atgtatatat cttattttta aatgtatata catttaattc
    17821 ttgatattat tatataccaa atgtatatac atttcaagaa aactttttca ttttatttaa
    17881 attttattcg acaacagcat tcaaagttaa tgtatatcca tagtgatatg tgtgtgagta
    17941 ctcaaaaatc tcactatttt gtaacttaca aatttggctt atatataaaa gtggctctgg
    18001 ttcttctaat tttagattat ccatacactc ttcattcgat attacagctt gtacttctct
    18061 ctctgacatg gctatcttgt atccacacac ttcttcaaca aaattataaa gagaatcttc
    18121 acaatgagtc ttatttaaat ttggaaataa cttaactggc atataagtaa tcatatatga
    18181 aactcttgca tcattggcta atcttactct ttttatttcc caaacatttt cttctaaact
    18241 tatattcaac ttacttgata aattttcatc tgcctgaact atttcaagag ataccaatat
    18301 attagatact ttatagcctt tactttccat ctctttagta aatccactta cagacgatat
    18361 attatcatat atattatttg caataactat actacctaca cctctttgtc tagttatata
    18421 gccttcttta actagattat taagtgcaga ccttacagtc attctagtta cattaaattc
    18481 ttcactcagc tgtttttcag aagggattaa atccccttct ttcaaactgt ctgaatttat
    18541 taactctcta acatgatttt ctataacttt ataaattggc tctttcattt aattcactcc
    18601 ttattaaatc ttttatataa taagataatt ttatatacaa acaaagaaaa tgtctataca
    18661 tttaagataa tttaaatttt attcagaaat cttgtacaac tttccgagtt ttaataaaga
    18721 tataaataga cttatcatta atataccttt taatatactt gataaggtta tactccacca
    18781 tacaccattt agacccaaca tttctggtct agatataaag taagccaatg gtactcttgc
    18841 accagtcagt atagtaatta taatagaagg tatataggtt ctccctaatc ctttgaaagc
    18901 tcctgttgtt attatttcta aacacataaa aaattgagaa tagcctaata ttttaagata
    18961 aactgctcct ttttctattg cctcactttc atctataaaa atagaaaata tactctctcc
    19021 tgcaaacact aatactaaag tagttattat accaagtaca gaagatacag ttatagctat
    19081 tttgcaacct ttatttattc tagaatactt cttggcacca taattttgtc caacaaaact
    19141 gcctaatgca actgatagcc cttcagccgt catccaggaa attgcttcta tttgtgagcc
    19201 tactttttgt actgcaacag ccacaggccc ccatgatgct actattactc ctaatagcat
    19261 tgagaatact gtaaacatac cactttgtat tgctataggt aatcccaatt tatacagaac
    19321 tttataatat ttagtctcta tttctttaaa tagtcttaat ttaaaatatt cttctttact
    19381 tttaactatt atatatataa aacatgtagt aactactatc tgagcaaata cagttgcaat
    19441 agcagctcct gcaacaccaa gttttggagc aaatccccat ccaaaaatta atatagggtc
    19501 aagtataata ttagttatta atcctactgt attaatacga aaaggagttt tactatttcc
    19561 aagaccattg aaaattgcag taaatactgg attaataaaa taaaatacca tacctagagc
    19621 tactattatc aaatactgtc tagacatggt tataacttct aagtctccta atctaaaaaa
    19681 acctatcaat tgtttattaa gtactattaa agaaattgta tatattattg ctagtataat
    19741 atttatttct attgctgact tcatatatga tttagtagtg cttatattat ttgcaccaat
    19801 actttgtgct acttttatct ctccacctac ttttgaaatc ataataaatg ccattgcaag
    19861 ccatggaaaa aaaccagctg ttccaactgc agctactgct ttacttccgg ctttaccaac
    19921 ccatatcata tctatcatgt tataccccat ctgtataaat gaagtcccca ttattggaag
    19981 tgatagctta agtaattttt ctgtaattct tccttctgtt aaattaagtc tgtctttcat
    20041 tttccacctc ttttatatta aatttatttg cttatatagc tagattaatc attttatttg
    20101 attgagttca ctatccttca atttatatta tcatatctca ggattgcatc tcaatcatac
    20161 ttatgtttaa gatacagtat tttacctatc tatgataaaa ttaatactta tgaatataaa
    20221 tctagccatt tcatcaatac atattctaac ccttaactta acttcaatta agtatacata
    20281 tcgtaaccaa ttagaatatt ttatacggta tcttccatta ataaacaacc aatatcaatg
    20341 atttatatat aattttattt atacataaaa tagtatacta taataataaa aaaactatat
    20401 ttgaatgctt taagcatgtt tactaattgt aatttccaca caaatttgat aatatatgaa
    20461 tatattatgg tgagatttat agatatatat taattatggg gagattgtgg gaggttcttt
    20521 tcacaagaca tttaagctat gaaaataaat aaaaaattca atagagttga tatgcaggtt
    20581 tcaatactta ctgcaattat tgtaataata tcttcctcat gtgtatattt ttttaattat
    20641 acactaactt ataagagcat gatatactct ttaagtgaga ggtcaaaaca tatctatgaa
    20701 tatgtagaaa gaaatattga taaaaatacc tttactgaaa ttaattctaa agaagaccaa
    20761 tcaaaaaagt cttataaaag ttctaaaatc cttttagaat ctgtaaaaaa tactactgga
    20821 gtcatgtatc tatatactgc taaaaaaaca aaagatggat cactagtata tgttgttgat
    20881 ggtttaaatt catctaggag tgattttaga aatgctggtg atttgataga aaaagagatt
    20941 tggggagacc ttaacaaagc attagataat catatattgt tacctaaaaa gattaaatca
    21001 acatcttggg gatacatatt tatatcgtat tttcctattc acaatgatgt tggtaaggtt
    21061 gttggagtaa taggaattga atttgaagca aaacatcaat acaatacatt taaatttata
    21121 agtatagtta caccaataat agcattatta acttgtctaa tatcagcatt gattgcagtg
    21181 attctattta aaagaatatc taatcctaca tatactgatt ttgctaatac agattattta
    21241 acaggcctaa aaaatagaaa tgcttttgaa atagatatga ataacttaaa caattcaaat
    21301 ctaaagaatc taatatctat catatcaata gatttagacg gtttaaaaaa gattaatgat
    21361 aaatttggcc atcagacagg agacttatat attaagcaag caagtaaaat tatacaatat
    21421 gtaataaata aaaaatatat agtttatcgt attggaggtg atgaatacat aattattcta
    21481 aaaaatttat ctcacaaaga aataaataat attataaata atattaattt aaaaatagtt
    21541 gaagaaaact caactaatga attaaaatta tcaatgtcta ttggatatgc tatttttgat
    21601 gaaaaagtag atgtttcttt atttgataca tatcgtcgtg cagattctca aatgtatgat
    21661 aacaaaagga aaattaataa acaatatcaa taataaattt aaaaagtcta ttctttaaat
    21721 aataaatcac aagaatagac ttttttaaat ttgtgttttt ataagataaa ttatttaatt
    21781 aaagtttgtt cttttttcta aacgattctt tataaattca tatattactt tcactaatat
    21841 atttgtgact aatattaaca cagctagtgc tgctgcctgt gcagtactcc ccttatcatc
    21901 tagttgtaca attgatattg cagctacttt tgaacttgca gtatatataa aaactaatgc
    21961 tgatattgtc accatggagt ttaggaaaaa gtacataaag ttctctaaaa ctgctggcaa
    22021 acaaagtggt atagttacgt tcttaagaat tgatgcgctg tttattccca ttgatttagc
    22081 taccatatct aattctttat ctactttctt aatagcagta gtagctgtta taaatgctac
    22141 tgaaaagaaa tgaactatat tacatataac cattataagt aatgttccat ataatccatt
    22201 aaacaaattc ttaatgtata ttgtatccat aaaattaaac tctaatttat taaaaaataa
    22261 tacataagct atacctaaaa ctaatcctgg caatgccata ggcaatgtac ttaaaaaata
    22321 cccaaatgat tttaaatatg gagctttttc tgttttttca ttcaaatatg caaataagaa
    22381 aacaaagctc gtccctagaa tagctgtaaa tagtgacatt tttattgaat ttataaatgg
    22441 tttaatccca tcaatactgt ttttcataga aaaatgttcc agtgtcagtc ttaaatcata
    22501 tggccaaact gatattaatg aagataagat tacagttaac attacagcta ttataccaaa
    22561 tgcaataatt cctacaaccc ctcctaaaat actatctctt aacttattag ttttaacctt
    22621 atatggaatt gactttgaag ttatatatga attttgattt ttttcaattt ttttagttac
    22681 tataaacgat attattgcag gtattaaaag taatattgat acagttgctc ccattggcat
    22741 attctgttgc cctataattt gcttatatac atctattgat aatacattaa actctcctcc
    22801 tatcaccttt ggagctccaa aatctgtaaa acataatgta aacgatacga atgcagaact
    22861 tattaaagag tattttatac ttgggattgt tatgcttata aattctctta tcttgcttat
    22921 ccccatagta ttactagctt catatagtct ataatcggtt gaacttagag atatataaag
    22981 tatttgaaag gattgaggaa atacatatat aatttgtgcc aatattattc ccactgctcc
    23041 atatattgga acatgcactc caagtaaatt agttaccaaa ccttgatttc caaataaata
    23101 tactagacct attgcatgca tcattgttgg agcaaacata ggaaacatag ctaatgattt
    23161 aaaaaaacct ttaaatttaa tctttgttct agttaatgca tatgcatata ctaatgcaag
    23221 tgttagtgat attacagttg atataactga tacaaatact gtatttttta gagaatttaa
    23281 tagagattca gaagccaagt attcttgaaa gttttgtaat cctacaaaga ctccatcttt
    23341 atctaaaaaa gcctgtacaa atagatatat tataggtgct aataaaaata ttactaaaga
    23401 cataactaat actgtaagca tacatttttt tatatctaaa ttcatttttg atttaagact
    23461 tatactttta gaacttatag cattattatt catacactta tgctccttta acttttggat
    23521 actttaaata gtatttttcg tctatactta tttttacaag gtcaccttct actaaattta
    23581 aattaaccca ctcttttata gaaacatcac ttattatgaa attttcattt aatttatctt
    23641 ttatttcaac tctcaataaa ttccctctaa actctatatt tgatattatc ccttggtagt
    23701 tttcttttgt cgatttctct atttgaacat attctggtct tacagttaat atagaatctc
    23761 cattatcaaa acagtttgta tctcctataa attgagctgt aaataggttt tgaggatttt
    23821 ggtatatttc ttctggagtc cctatttgca ttatatctcc tccatttaga atagctattt
    23881 tatctgccat agttatagct tcttcttggt catgtgttac cattattgtc gttatattaa
    23941 gttctttttg taatctttta atgtccattc taagcttgtg tctaaccttt gcatctaatg
    24001 cagacattgg ttcatctaaa agtaaaaact ttggttcaag agctaatgct cttgctattg
    24061 caatcctctg ttgttgtcca cctgataatg ctttagggta tttatgagct tcattagtca
    24121 atccaactgt ttcaagtact tccttaactt tattatctat tttttctttt gaaacttttc
    24181 tctcttttag tggaaaagct atattgttat aagcagtcat attcggaaat aatgcatatg
    24241 attgaaatac tataccaaaa cctctctttg atggctctaa atttgtaata tctttatcct
    24301 ggagaattat agttccacta tttacatctt caagacctgc tattattcta agtaatgtag
    24361 ttttaccaca tccactaggt ccgagtaaac atagaaattc cccttcttca atatccaaac
    24421 taatattatt taaaaccttt ttttggtcat aacttttaaa cacattattt atttttaagt
    24481 aactcatatc ttatctctcc ttatatagtt atttttttat tttaaatttt atatcctact
    24541 ttttcttcta aaattctttc atctaaatta ttaatttaaa ctttacttac tttcagattt
    24601 tgaaccgtat tttgattccc aagttttaag tactgtactt ctgtttttgg cagcagagtt
    24661 taaatcaact tcttttataa gctgttcaaa tggttgttta gaataacctt ttggaactgg
    24721 attatctgtc tttatggaag taactgctac ttcatgtgca tatctatcca tagctgaatc
    24781 acttattgcc caatctaaaa atagctttgc ttcttttttt atattatctt ttttaactaa
    24841 ggcatttgct tctaagtccc aacctgaacc ttcctttgga aatacaacct ctataggttc
    24901 tccactagat aattgttgtg tagctctata tccaagtgta atacctattg gatactctcc
    24961 agagccagct aacttcgcag gttttgaacc tgagtgtgta tatgtggcta tatttttatg
    25021 taatttatcc atgtaattgt aagcatcttt ttcacccatc atttgaataa gccctgatac
    25081 tgtaagatac cccgttccag atgatgctgg atttggcatt gttataagtc ctttatattc
    25141 tggttttatt aaatcttcat aactttttgg aatatctata cctagtttct taagttcctt
    25201 ataattaact actattgctg tctctgggac actcattcct acccaagaca catcatcttc
    25261 agtatcttta aattttttat caactttatc ataattttta ggcttatatg cttcaagtgc
    25321 accatctttt tttagctcta tcatgttcat tgctgataat ccccatataa tatctgcttg
    25381 tggattatct ttttctgcta atagttttga agctattaca ccatgagaat ccataactat
    25441 ctctacatct atattgggat actcttctct aaaaccttcc atataataag ctatctgctc
    25501 ttcctctagt gctgcatata ttgtaagttt attatttcct tttccttgag tagtttgtgg
    25561 tgttgttgat atgcaacttg tcataaatgt aatcactaca atgcacagac aagtaaatat
    25621 aaattctttc atatcttccc cctttgtgtt ttttcctttt tttatttgtt ttttattgtt
    25681 tttgcttatg tttatataat acacctaatt tgttaatata aagttaagtg taaattaaca
    25741 gttcgttaat ttttagaaat ttttgttaaa tatattattt ttagttaaat acagtctgtt
    25801 tttaaacaat atattaattt ttatagtatc tacattttaa tttaaacata aaaaataagt
    25861 ctaacctcta taaagtagaa tgttagactt atttttataa gtatttaatt atacaaccaa
    25921 atcatttttt ttcttttctt caagtgcttt tttaagcacc atatccttaa ccttcataag
    25981 tctagtagta cttgctctct cattttcttc aagcttcata gctatatact taatagtctc
    26041 aatgtaattt ggtatcatta cattttcaag agcattaaca cgtctacgtg tcttttctat
    26101 ctcttgtgca agaagctgtg cacttttttc actttctgca agtcttaaaa gtggttgcat
    26161 agcatctgag aaagcttcca tagcagaatc taattcacct gatgtaaaag caagaccata
    26221 aggataaata tcactttgat tgttttcagt tttaaagtca aacacaggaa catcaacact
    26281 cattatattt ctcgtagaaa catctactga aactgattgc tttggcatca taagtgctga
    26341 acccagatac tcctgactca taactgctct tgcgattata aagtttttat aagcagtatc
    26401 aagagcattt tctgcttctt cacgcaatcg cttattttct cttacaatct caagaaattg
    26461 tttcataagt tcatcaagtt tatctttaag aagcttatga cctcttgtag cagttttaag
    26521 gagtttctta agtctagtca tttccatacg tgttggattt atatttaatc tagccacaga
    26581 tatcactcct taggcatata tttttcaaaa tattcatcac ggatacgttt aagttcactt
    26641 tttggtaaca tactaagaag cttccatccg atttctaatg tctgctcaat agttctatca
    26701 gttctaaatc cttgtgaaac atattctttc tcaaattcat ctgcaaactt agcatacatt
    26761 aaatcaatct cagaaagtgc agattcacca agtatagcca taagttcttt tgcatcctta
    26821 cctcttgaat aagctgcaaa aagctggttc attgtatctg catggtcttc acgagtctta
    26881 cctttaccaa tacctttatc ttttagacga gaaagtgatg gtaaaacatc tataggaggc
    26941 tgtatatctt ttttataaag ttcacggcta agtataattt gcccttctgt aatgtatcct
    27001 gtaaggtcag gaattggatg tgtcttatca tcctcaggca ttgtaagtat tggaatcata
    27061 gttatagacc cttcatgccc tttcattttt cctgctcttt catacatagt agcaaggtca
    27121 gtataaagat atcctggata tccacgacgt cctggaactt cttttttagc tgctgaaact
    27181 tcacgcaatg cctctgcata gtttgtaata tccgttatta taacaagtac atgcatatct
    27241 tgctcgaaag caaggtattc tgctgctgta agtgccattc taggtgtaga tacacgctct
    27301 acagctgggt catttgcaag gtttacaaac actacagaac ggtcgattgc accagttgct
    27361 ttaaaatcgc ttataaaaaa ttcagcatct tcaaaagtta taccaactgc agcaaataca
    27421 acggcgaact tagaatcagt tccacgtact tttgcctgtc ttgcaatctg aactgcaaga
    27481 ttggcatgtg gcaaaccaga ccctgagaat ataggaagtt tttgccctct aaccaatgta
    27541 ttaagtccat ctatagcaga gactccagtt tgtataaact cggccggata atcacgtgct
    27601 gctggattta ttggagctcc atttatatca agtcttttat ctggtataat ttctggacct
    27661 ccatcaatag gtctacccat tccactaaaa acacgaccta atatatctgg tgaaactcca
    27721 aagtcaagac tctttcctaa aaaacgaact ttactttcag caaggtttat acctgttgag
    27781 ctttcgaaaa gctgaacaag tgcagtatct ccatttacct ctagaacttt gcaacggcga
    27841 atttctccat ttgaaagttc aatttctcca agttcatcaa attttacacc ttctacgccg
    27901 tttattaaca taagaggacc ggctacctct tgtattgact tatattcctt tatcatagtt
    27961 cttcagcccc cctcttttta ataaggtctg caagctcgtt gtctatttca tcttcaattg
    28021 aatcgtaagc tgcatctata tcgccttctt ctacatactt agcacgacca atcttttcaa
    28081 gaactgaaag cttgataata tcgtttatag taacattttg cttgatagca tcttgtgctg
    28141 acttatagaa ttttaatata agtcccatca ttctatattg tttatgaagt gatgtataag
    28201 tatcaacttc atggaaagag ttttgatgaa ggaagtcttc tcttatagaa cgagtaacct
    28261 ctagtatcaa tctatctcca tctgaaagtg aatcaacacc aaccaattga actatttctt
    28321 gaagttcaga ttcaacttgt aaaagtttca tagcttgagc tctttgagct gagaaattat
    28381 catttacatt tttatctgcc catacatcaa ctttttcctg atagagtgaa taactagtca
    28441 accagttaat tgctgggaaa tgacgtttat atgctaaaga tgcatcaaga ccccagaata
    28501 cttttactat acgaagagtt gcttggctga caggttctga tgtatcacca ccaggaggag
    28561 aaactgcacc aattgctgta agtgttcctt ctctttcatc catacctaga caatttacct
    28621 ttcctgctct ctcatagaat tgagcaagac gtgaacctaa gtatgcagga taaccttctt
    28681 caccaggcat ctcctctaaa cgaccactca tctctctaag agcctcagcc catcttgatg
    28741 tagagtctgc cataagtgca acactatatc ccatatctct aaaatattca gctattgtaa
    28801 taccagtgta tatagaagct tcacgtgcag caaccggcat atctgatgta tttgctataa
    28861 gcacagttct ttgcataagt gactcgcctg ttcttgggtc ttttaattca ggaaattcaa
    28921 gaagaacatc tgtcatttca tttccacgct cgccacagcc tatatataca actatatctg
    28981 catctgccca tttagcaagc tgatgctgtg taacagtttt tccacttcca aaaggtcccg
    29041 gtatagctgc taccccacct tttgtgattg ggaaaaatgt atctataact ctttgacctg
    29101 taacaagaag tgaatctggt gttttctttt ctctatatgg tctttctttt ctaactggcc
    29161 atttttgcat catagttaca ggtatatcat tacctttttc atctgtaata acacaaactg
    29221 tatcttcaac agtaaattca ccagaatata tttcttttat agtaccctta actccataag
    29281 gaaccataat tttacattct actactgctg tttcttgaac gccaccaatt atatctcctg
    29341 ctacaacctt atcaccaaca gattttttag gtttaaattc ccacttagtt gttctatcaa
    29401 gtgaagatac ttcaacacct ttagttatat tagtacctga aatatcatac attttttcaa
    29461 gtggacgttg aataccatca taaatagttg aaataagacc tggtccaagt tcaacactca
    29521 taggcattcc aagagaaact acttcttccc ctggtcctag accggaagtt tcctcataaa
    29581 cctgtataga agctttatct ccatgcattt ctataatctc gcctataaga tgtttatctg
    29641 aaactcttac gacgtcaaac atattcgcat ctctcatgcc ctccgctaca acaagcggtc
    29701 ctgacacctt tacaattgtg cctgttttca tattctctca cctgcaatca tatactttat
    29761 caaaatttaa atattccctc ctatatttta ttagcacata tatttagcac caataaaatt
    29821 tgagattgtc ttttattcaa ctttcatcta ttctttaaat aatatatctg cacctatagc
    29881 acgtttacta gattctctta cttcattcat acctaatccc atacttcctc caatacctgg
    29941 aataactata atggcaggaa gttggtaatc tttgtatttt gctattgatt cttttgcaag
    30001 caatgatgcc tgttctgtaa tataaattat cgcatattca tcttcaacca acttatgtat
    30061 acttttttta atttcgtcac tatcataggc tgggaagata tctattccaa gagctaaaaa
    30121 tcccatgatg ctatctttat ctccaactac tcctacctta tacataagca tcccttagcc
    30181 tttcttttat agtatctgcg tctatattat taagcttact agtcaaaata attctaattg
    30241 tttttatctc tgattcttta gcatataaat atgcaattag tggttctaat gtaagaggct
    30301 taaattttgc tcctctaaca tattccataa gatagtcatc acaaagtttt tcaaagacag
    30361 taaaaccact atccatgcca tttttacaga catcagagta agaagtagat ttaaaacatg
    30421 atgctggagt atctgaggaa aaagcttcta agaatttttc cttatcaagt gagccacctg
    30481 aaacaaacac attcataaac atatcaaaat ctttcttcat atttttgact cttataaaac
    30541 tctttagatt tgttaaatca caaactttca taacataatc tattacaaat ttgttttggc
    30601 tttctttagc agtttctttc atactggtaa aggttgcttt atctagaaca atatcaacca
    30661 tctggccatt ttgtgtctta ttataaacat caaatgcctc taatatagct gatgacatta
    30721 tcttaggtaa atcactaaaa ctacgatttg ctaaagattc tctaagctta ataagaggaa
    30781 ttgtacctcc atcaattaaa tatttctcac catctacacc agaaatttct tctttaatca
    30841 gcacttttaa attgtgatag tcatttttat aaagaaatac atcaacaaat ttttcttcag
    30901 gagctagact tttaagaatt tcatatgtct ttgataattc ttgagtcaaa atattctcaa
    30961 aattatgtat attattattt gatgtatcag cataacctgc ctcagctata attcttaaag
    31021 agtcttcagg gcttttggct tctgccattt gaataaacat ttgtctgtta agaagctgtt
    31081 tttcaagcac tcttattcgt gcaacggcat agggataagt tttttcttct gccatttttt
    31141 aacccctcct taattaaaca aaatttctgc tgcaatttgt tcaatatatt catgctctac
    31201 tgcaattatg gcttcaaatg aataattgta ctcaatgtca ccttttttaa ctatgaaacc
    31261 tccagtaata tctctagttt catcagacac tttaatgcct ttttttgata gcacatcttt
    31321 taatgttttt ttatcttttt tagataatac tatctctgaa tcatctgtac aatttgattt
    31381 ttcaatcatg tttagtataa tctcttcata ttcattatct ttaagattta aaagtttgtt
    31441 tttagcttct aaaattacct cttctaaaat ctcttgtttt tttgataata tttgcttttt
    31501 agcctccata taagctcctg atatttcctt agaagccgct ttttctgctt ctgcctctgc
    31561 aagctttgta taggaagcca tttccttttc agctttttca ttagctgaat ttacttttaa
    31621 atctgcttca cttttagctt tatccaatat ttcttgtgct tcttgtttgg catcagctat
    31681 aattctgtca atcatttttt gttcattacc cataggagtg cttcctcctt ttcatttttt
    31741 ttacttatga attataattt ataattaacc aattgcaatt ccattgtaag ctaagaaaga
    31801 aacaagtagt cctaaaagag catatgtttc aactattgct gcaaatgtta tagctttacc
    31861 aagttcttca ggtctttttg caactattcc aactcctgct gctgctgctt taccttgtgc
    31921 tatacctgat actaaaccaa ctaaaccaat tggaagtcct gccataagaa gttgtaaacc
    31981 ttgagctgat gttggtattg catctccacc aataatccct gcttttgata aaataaggaa
    32041 agctatgata aatccataca gaccttgtgt accaggaaga agttgtaaaa ctagaagttg
    32101 accgaattta ctagggtctt ctgttacaac acctgctgcg gcttgacctg ctattgaagt
    32161 acctttagca gaacccatac ctgcaaataa tgctgctata gcagctcctg atattgccaa
    32221 aaattgacca tttcctaacg cgtttaaaaa ttccatattt acatctcctt tttattaatg
    32281 cttgtatatt ttgttttata cttaaacgga acaaaaggaa ctcctccgcc ttcataaaac
    32341 ttattgaaaa attctacata ctgtaaccta cttgtatgaa catatgcacc tagtgcattt
    32401 ataagaaggt ttattgtgtg tcctacaaca ccaattacaa ctgcaagtat cggaccagct
    32461 atagctccta aaaggttgat aacttgtgct ataactcctg ttgttaaaca aagcgccata
    32521 attcttgtat aagataatat atcagcaaaa taacttgtta taccatagag acttgaaaat
    32581 cctttaaaaa gcttaacagg aataccttta aagcttcttc cttgagtaag tacaagacct
    32641 atagcaccta taattgctaa atattttcct acttctgaaa atacacctat gtcccctgca
    32701 acaaaaggta ttataagtag acatacacct gttatacaca aataccaaaa acctatgtca
    32761 aagattgcat ctattacctt tccacttctt attaatgtat atgcttttat tcccaaacct
    32821 acatatatat ggataatacc gaacagtaac gacattccca taagtgtcat aacatcctct
    32881 aaaggattaa ttaatgcttt tataggaatt aaatccccca aaataccccc gaatataaga
    32941 ccccatacag tagttgaaac tccacagata ccaataagtt taattaaatt tccttcacct
    33001 ttttgtatct ttaatttata tacaacaaat ccacaaacta ctgcaattat taacccatat
    33061 gcggcgtcac ttagcatcat tccaaagaat acaacaaaga agaaagttaa aattgtattt
    33121 gggtcaatat cttttgtact tggatagctg tacatgttag ttacactttc aaaaggtgtt
    33181 actaatttat tgttttcaag cagaattggg aagccttctt ctttacttcc ttcttcagtc
    33241 tgaacacaac aatcaaactt ttctgtaatt tctttaatta attcatctgc ccttttgctt
    33301 ggtaaccaac cattaagaca gaatgttgtt tttgttttga caagtctctc tataatcttt
    33361 ttttggtctc tttctatagt gtaaaaatca tataggtttt caagcatgtg tatactatct
    33421 gcatgtcttt taatttcctc tgttttgtcc ttagaaacgt tttcctgtct aattatcatt
    33481 tcattgtatt tcttgatagc ttgtaacggt gtaccttctt catttggtaa tgaaaccact
    33541 gaaaaattaa attcttttaa aacttctaaa acatcatcaa aagtttcttt taaagtaatt
    33601 aaataaacat agttcattaa cttgtcagaa ttgacaatcc caataacact ttctggtatt
    33661 tcatcttcta attttactct taaagcatca atgtttgcgc ttatgggcac agttccaagt
    33721 atagtttttg tatacttagt ttccatattt tcaataggaa tatcaaagtt cacccatggt
    33781 tctaacatac ttttattgtt gttaagtaag tttatgttag accttattgt acctatctcc
    33841 ttgtgcaaat tgtttaactc cagagcttca gcataaactt gcccttcttt ttcactatca
    33901 attttgttaa actctctttt tggtgcaagt agggattttt tttgtttcac tagtgaagaa
    33961 acaatattaa ttgcttggtt tacatgagac attttctgct ctagcattat aacttcagat
    34021 tcttcactat cttttacaag ataatttttt aattcctcct cctcagtaag aaaggcacta
    34081 tcatcaatct gaacaaaccc acggttcata agtagcttta aaatctggct tttttgagct
    34141 tctaagccaa caatagatat tttgttcata ttaactattg ccatattatc tcactacctt
    34201 tccaataacc gcttcaatag ccttactttt tctttcttca gcagtttttt caattttcag
    34261 acattcactt ttagcattag ctaagatttt atcaacctct gctctactgt cggtgactgc
    34321 cttctcaata agagtttctt ccataagctt tgcttgtttt tttacatcag caaatatgct
    34381 ttcacatttg tttacagcat cagctctgat agactctgca tcagtaacag cttttacttt
    34441 gatactgtct gctgtctgct ctgcctcaac aatgcttttt attatttcaa aagccatcca
    34501 aatcacctct cttacctttt tactatgtat ataaccaatt agattttttt attctaatta
    34561 gattactacc tcattgttaa agtataaaca gcatttttat ataataatta atatttttca
    34621 acgtttattt cttaataaaa catattttag aatagtactt gcctagattt acatcctttt
    34681 ttgacttact tttagactat gacatgagta tggtttttaa ataccattat tttcaataat
    34741 tataatttaa agcgatatag tttgtcaata gcgttatttt gttttaaaat taacgttatg
    34801 tcgacatttt aaatttttat tttatttttt ttataatttt aatttaattt attacatata
    34861 aaagtttttt atattctttt tcaacctcta accattgaag tttattgttt tttttgaatt
    34921 tttagaaata tctcgcacta ttttatctaa cttttataaa tcatcaagct atatcgattt
    34981 ttgtcacctt atttttaatc taattttttt atatctagtt agaaataaat caatattttt
    35041 aaagattgat aaaacttaca cctagttcta atagttttat tttaaggcaa tgtattaatc
    35101 ttaagtagat aatatcatta gttactctat atattcgaca acattataaa aaaatcctcg
    35161 taccaatcac tccaatcttc aataggatat gctgctcctc catttacact tttcagtaga
    35221 ttggatgtat attcttctcc catagaaaaa agttccccta ctgtcatatc ataacctcta
    35281 gatgtaacaa ccttacaaaa tgaatccata acatcacgtt gttctcttgt ttttataagt
    35341 tcctttctaa attcatcatc attcttagct tcttctaaaa ttttagttaa tttttcattc
    35401 attatgtatt tctcctttta agtagtcttt ctgtaaactt caataagctt tatataaaaa
    35461 ggaagccatc ctaaataaac taagatgact tcgcttaact gttagcaaca catttactag
    35521 tataaaataa ttggtgtacc tggttctata ttttcatata ttttctttgc cttagaaaaa
    35581 ggtgtattta cacatccatg tgaacctgaa gacttatata tacttccacc aaaagaattt
    35641 ctccaagtag catcatgaat gcctatgttc ccattaaatg gaagccaata acttacaggt
    35701 gaactgtaac cttgaccagt taatgaaaca tttctagcct tataattaag aggatatata
    35761 cctgatggtg tagcaaatcc tctagctaaa tcacctgtta caacgtctgt tgatacaagc
    35821 atttctccat ttttaaagaa ccacatatgt tggccagaaa gactaatctc tacatatgta
    35881 tttccaatat catctttatt acgagataca gctttttgta tgtaaatagg ttctcttgta
    35941 acatttttac cagcttttac aacatcaact aatgcttcag cttctttctt tttatctatc
    36001 aaccaaccat aaattccacc tgaaattgta atatctttac ctgtggcagt tttaaatggt
    36061 cttgagtcac caaaagtact gtatttttta cttaaacctc ttgtatattc taccatagca
    36121 tcattatcaa aagttatgtc ataattcttt tctgagtcta cttttaacca tttattaatt
    36181 tcagaaccat ctaaagtttc cttacggtca ctaaaatcat atgtaacgac tacaccaaca
    36241 tacttatcta atgtcttaat agcttgtttc aacttttctg aatcctttgt gttgtttggt
    36301 gcttcataaa ctttactttc atccagagaa atagaagtat tcccagaatt aattgagttt
    36361 tgtactaact tataaaaatc atcttttttc actttgtttc caagtacctc tggtactata
    36421 acataagcgt ttttagtgct atcatactta ggataagcac tcttaggttc tacaacattt
    36481 ttactatcaa aacaagataa agcattaaac cttttttcta gtaaatcttt attatatgaa
    36541 agagttatca tattattata ctctttagat ttaaatattc caccaatcca tccaatagcg
    36601 ctctgactat cttttatctt ttgaacttta tcatctggtg taaattttaa atcaatatct
    36661 tttgctttta tttgttcttt cttattgttt ctttcctcta agtttaaagt ataggaatta
    36721 gtactagatg ctaacaaatc atttacttgc tcaactgttt ttcctgaagc ctttacgcca
    36781 ttaatagtcg ttccaaagta aaaatgattt ctaaaaaaga ttgccattcc tatataagct
    36841 acaaacaaaa cacctactac tatagctatc ttaaatttta gatttttttg tccactttct
    36901 tgttttacct cttttccatc aatcataatt aaatccttcc ttacattgta attacctatg
    36961 taccttattg gtttttaaac aaaaccatgt atgccttatt ttatataccc aaattactgt
    37021 atcattattt atatttttaa atatatagtt atttctataa aaccatttta tcttttagat
    37081 aaaatgatta ttataaatcc cccctttcaa aatcccttaa acacaatctc tgctatatta
    37141 atatattacc atacttttaa taatcgtggg actatctatc tatttttgta acactaaact
    37201 actattttaa aaaagaaact aattttgtgt tagtttcttt atcttaactt atttttatat
    37261 tgaattttag cttaacataa cgttgttata tagattctat ttaattattt tagtttctat
    37321 atcttttcca tctgcatatt ctttaagtaa ttccttagca tatccaaaat attcttcaat
    37381 atcagattct tgactaaaag aaaatataca aattaatatt tcatttgcat cttctgttat
    37441 cattgctctt tcagaatcac tagttggact cccaaattta cccactgaat cagacaatat
    37501 aggaagattt tctatattta ttaattcttt cccaataccc ttatactgtt ctccttcatc
    37561 tcctacagta aaacatactg gtgaatgtaa tttgcttaca ttatatgtac ctactggata
    37621 taatgatttt attgatatta aattgtttat ttctactata ttatttattg tataaagacc
    37681 cttcccttga ataattcttc taaccaatgc ttctgaagat gttctatatc tacttggatt
    37741 ttttccaagc tctttataag catttcttgc atctttaatt ttatctagtt tcgtaatgtc
    37801 ttcaactttt agactttgat ttatagtatc acaaaactca tttatttcat taattaattt
    37861 ttctgagctt ttatttactg ctacttttgc ttgaatgcaa cccagcttag cattaggcca
    37921 tctttcctta agtttgtcat caatacttat atctatcata cttaaacaac tcctatacac
    37981 taaattttta ttaaagtttt tatcatctat acattatagt a
//
LOCUS       DAKBNN010000013        35671 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000013 DAKBNN010000000
VERSION     DAKBNN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.013"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..707
                     /locus_tag="OUB02_000683"
     CDS             <1..707
                     /locus_tag="OUB02_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HCT8884480.1"
                     /translation="TGATGATGATGPTGADGATGPTGATGNTGADGVAGPTGATGATG
                     NTGATGPTGATGATGATGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSI
                     VGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTA
                     PNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAQTRFLLVFTATASGLS
                     LVNTVAGYASAGIAIN"
     gene            876..1637
                     /locus_tag="OUB02_000684"
     CDS             876..1637
                     /locus_tag="OUB02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HCT8884481.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIIAYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1946..2224
                     /locus_tag="OUB02_000685"
     CDS             1946..2224
                     /locus_tag="OUB02_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884482.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2517..3092
                     /locus_tag="OUB02_000686"
     CDS             2517..3092
                     /locus_tag="OUB02_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HCT8884483.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            3531..4241
                     /locus_tag="OUB02_000687"
     CDS             3531..4241
                     /locus_tag="OUB02_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884484.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            4235..5551
                     /locus_tag="OUB02_000688"
     CDS             4235..5551
                     /locus_tag="OUB02_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT8884485.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKCIQ
                     TSKDDNKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            5563..5703
                     /locus_tag="OUB02_000689"
     CDS             5563..5703
                     /locus_tag="OUB02_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCT8884486.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            5762..6339
                     /locus_tag="OUB02_000690"
                     /pseudo
     CDS             5762..6339
                     /locus_tag="OUB02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
     gene            6596..6970
                     /locus_tag="OUB02_000691"
     CDS             6596..6970
                     /locus_tag="OUB02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884487.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            7053..7262
                     /locus_tag="OUB02_000692"
                     /pseudo
     CDS             7053..7262
                     /locus_tag="OUB02_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7557..10601
                     /locus_tag="OUB02_000693"
     CDS             7557..10601
                     /locus_tag="OUB02_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HCT8884488.1"
                     /translation="MKKILKRLATGFLAFATIVTALPTTAVHASEKQYWTESAERVGI
                     VEKVMNDGSIGSTFNEGHMTVEGEDAFCVDINTNFRNGYKTRADASTRMSYDQISDVA
                     LSIEYVNQYVQSHSGLSSQHIYLLKQCVVWQRLSVHLGWQCDNVRASYDEIPKAIQDE
                     VYAGAKAFASENKERYECGGYIYSGEGQDIGQFWAKLAVGNATLKKTSSNASITDGNG
                     LYSIAGATYGVYSDKDCTKQLATLTTDNSGNTDTVEVKAGTVYIKELSAPAGYKMDST
                     VYSLNVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDAPQGDASLEGAEFTWKFYA
                     GHYTADNLPSEPTKTWVTKTIAEKDSDGTIHYVSRLSDEYKVSGDSFYTQDGKNVLPL
                     GTLTVEETKAPNGYLLDGAYMQTDGSEEQIKGMYLTQITEDGDIAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETSDTKGQGSATLKDAEFEIISLNDNAVLVEGKLYSKNEVVKTIL
                     TDIEGVASTSADLLPYGKYRIEESKAPEGYLTDGAEPIEFEITEDGKIVDLTGTDTSI
                     YNQVKRGDLEGIKIGAGTHQRLAEVPFRITSKTTGESHIIVTDDNGQFSTSSDWASHK
                     NNTNAGKTSEDGIWFGTSEPDDSKGALIYDTYIIEELRCDSNKGFELIPAFEIVVSRN
                     NVTVDLGTLTDEYEKEISIHTTATGKDGEKSIVAGKEVTIVDTVTLDGLEKGTKYQLK
                     GWQMLKEENAELLIDGQRVESDYTFTADSEEMKIEIEYTFNASSLGGQNLVTFEELYD
                     LSNEDEPVKVAEHKDIDDEGQTVLITERIISIHTTATSEDGKKEIEAGKDVTIIDTVT
                     LDGLEIGTKYQLKGWQMIKDENAELIINGERVENDYTFTADSESMEVQIAFTFDASEL
                     GGKELVTFEELYDLSNPDEPTKVTEHKDIEDDGQTVTITEVPEMPEEPTEPEQPTTPD
                     TPSKTTDAPKTGDNTNIALFLGLLILSGAGVAGTYFIKRRRS"
     gene            10837..11163
                     /locus_tag="OUB02_000694"
     CDS             10837..11163
                     /locus_tag="OUB02_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT8884489.1"
                     /translation="MKLKYIVPDMAQTFGNLEFAGENEVEQQRVNGRFVPVTRSYNLY
                     SDIQRADDIIVVLPAKAGEKRFKYEQKVKLVNPKITAEGKNVRGRGYTNYILNADDML
                     PVEESK"
     gene            11180..11566
                     /locus_tag="OUB02_000695"
     CDS             11180..11566
                     /locus_tag="OUB02_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT8884490.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRVENEDGTTTEEIKRRT
                     YDLKCSQQERTIQVSIPAEIPVKDYPYNAEVELINPVADTVANANYRGADVEWYIKAD
                     DIVLKNKGGQPAGNPQNHAPQGQQKK"
     gene            11698..12540
                     /locus_tag="OUB02_000696"
     CDS             11698..12540
                     /locus_tag="OUB02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCT8884491.1"
                     /translation="MYWLGLIGFLGTFLDIPVLQLFYLFFLLAIVDFVLSFIIVIQTE
                     STDETAIALKFLSQNIGMLIGIPVIYVRNLFFLPNIRNYKPQTLYSLPFTGCWTVANG
                     GINRETSHSWSICNQRYAYDFFVEENGKTFHGNGKAVSDYFCYGKPVLSPADGIVVEI
                     KNLFEDTPIPEEQEVLCSASDVRGNYIVIQHTKHEYSTIAHIKKDSFCVNVGDKVCRG
                     QQIACCGNSGNTSEPHIHFQVQQGKSFLFSASLPIWFTKIINHTISKADSHISRGQIV
                     ENQK"
     gene            12601..13983
                     /locus_tag="OUB02_000697"
     CDS             12601..13983
                     /locus_tag="OUB02_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCT8884492.1"
                     /translation="MNILKYKGKRIRASDKKLVYRFCAGTLLLLFVAVLLLLNMGQLM
                     RTDWEHFSLLDNGITLSAYNFITIGIATGVCALVAFLYYRFFHDSFKKLLHRQKLSRM
                     ILENKWYEAQTVQDSGFFTDLQSRSREKIVWFPKIYYQMDTGLLHIRCEISLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVKAENGGLRLMKNLT
                     WEYDALPHALICGGTGGGKTYFLLTIIEALLQTNAQLFILDPKNADLADLGTVMDNVY
                     HTKEDMIECVNAFYEGMVARSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFLEML
                     GTKESMSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYAPLVPKGHDFLQTIGSLALARQ
                     AVPEKQKEIE"
     gene            13980..14393
                     /locus_tag="OUB02_000698"
     CDS             13980..14393
                     /locus_tag="OUB02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884493.1"
                     /translation="MIMGYLEIHYEPECTDSVLTCIGLGYGKFLSDLAFTADSEYKQD
                     DDYPETLFHKRMSELLEDLAEDYLEMQLLFSVELPAHMANLLGCLFRYTFLVMDREHF
                     RQICREYEIDKDIARKCLSRDTDCIVVYTGMTRIG"
     gene            14644..15834
                     /locus_tag="OUB02_000699"
     CDS             14644..15834
                     /locus_tag="OUB02_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCT8884494.1"
                     /translation="MNESTFIIALKEKRLSYGVSQTRLAIMAGISREHLNRIEAGKVT
                     LTDDMQDKLMEAVEKFNPDAPMFLLFDYVRIRFPTLDIQHIIKDILKLNIDYMLHEDY
                     GHYKYTEHYYLGDVFVYTSQDEEKGTLLELKGKGCRQFESYLLAQERSWYDFLMDALV
                     EGGVMKRLDLAINDRAGILDIPDLTAKCNREECVSLFRSFKSYASGELVKHNEQDKAG
                     MGHTLYIGSLKSEVYFCCYEKNYEQYAKLGVPIEEAPIKNRFEIRLKDERAYYAVREL
                     LTHYDAEQTAFSIINHYIRFVDREPEKRKTDWKLNDRWAWFIGKDRPPIKLTTDPEPY
                     TLERTLGWISRQVAPTLKMLKKIDAGNSTSYLKEIEDNAKLTEKHLQIIRQQTADTEE
                     LITE"
     gene            15850..15990
                     /locus_tag="OUB02_000700"
     CDS             15850..15990
                     /locus_tag="OUB02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCT8884495.1"
                     /translation="MAELVKDLVLVSLGLGIGVVLMCIAQVSKMADEQMEEIKKERID
                     KE"
     gene            15987..16208
                     /locus_tag="OUB02_000701"
     CDS             15987..16208
                     /locus_tag="OUB02_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884496.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     IALIAVGLVFNAGGVKDVLLELFNRIIGA"
     gene            16413..17150
                     /locus_tag="OUB02_000702"
     CDS             16413..17150
                     /locus_tag="OUB02_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT8884497.1"
                     /translation="MDTKRLAKFLIITFVITGIAWSGLAVLTSLNIIPFSHPLGTILH
                     IIGGFGPTIATFFVLEEKNTVKSILHFIFGYRKKSLIFLFLFSIFEILTIGLSSREFN
                     SALPWYLMPLIFLQATFIYGGEEELGWRGVMQPLLEEKLNFPIATIITGVVWGIWHIP
                     LWFVNGSSQQNMPFLFFLALAVILSFWLATIYKKTKCVFACSVFHGLTNTLLSVFIIK
                     VNVLLVIGLIAMLVYSIYLWYCGEAKQ"
     gene            17205..17666
                     /locus_tag="OUB02_000703"
     CDS             17205..17666
                     /locus_tag="OUB02_000703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884498.1"
                     /translation="MKNVIYILIQCTWGLPQTLLGFIVFLTNIKNKHYFYHGAIITER
                     NVPSSVSLGMFVFTTTNPMKDKRTKNKIPDEELSKRLLVHEYGHTIQSLIFGPLYLIV
                     MGIPSTLWGFLPYFQNKRNNGVSYFSFFTEKLANYLGEKVTKEKSMENAII"
     gene            17693..17869
                     /locus_tag="OUB02_000704"
     CDS             17693..17869
                     /locus_tag="OUB02_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884499.1"
                     /translation="MFQELGISAGTAIIILITLYFIIKWAVKNGIKEAYQEIKKKSPE
                     DLRFERREKDINKS"
     gene            18172..18669
                     /locus_tag="OUB02_000705"
     CDS             18172..18669
                     /locus_tag="OUB02_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCT8884500.1"
                     /translation="MEEMRVQIEAREPTDGEIRAFWFTLPIDTEQVEELLGIDIDTDY
                     YYITGKELPFADEVQEDTTVEKLNDLYHTYENLPSDLKEDYTELMCYFSDLDELHRYR
                     NDIIHYSWCKNMTDVARHILNNDPDFTSLSESVTRYFDFEAYGQYLDDNGRFVETDHG
                     IYELP"
     gene            18687..19190
                     /locus_tag="OUB02_000706"
     CDS             18687..19190
                     /locus_tag="OUB02_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCT8884501.1"
                     /translation="MVDDMRVYIANLGKYNEGELVGDWFSFPIDEEDVAERIGLNSYY
                     EEYAVHDTDNFPIEIGEYISIEELNEMYDMICELPDYITDALDEFVSHYGSLEEVYEH
                     KDDIYFYPDCDDMTDIAYYFIDELQVLGEIPLPLQNYIDYEAYGRDLSIEGTFIETSR
                     GICEIPY"
     gene            19299..19688
                     /locus_tag="OUB02_000707"
     CDS             19299..19688
                     /locus_tag="OUB02_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT8884502.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFTLPFPVTFTQITWFVITEFAV
                     ILLGDLPPLSLIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKVTY
                     AGKAVKLEKEVFSEPVTAVRSVMYVPD"
     gene            19675..22122
                     /locus_tag="OUB02_000708"
     CDS             19675..22122
                     /locus_tag="OUB02_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8884503.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSVRSIQEQSKRLVTGRLRDVAIQKINEQTEALVSMI
                     GDNQVDYRFFIGFKLIVTEEKVSLDSVKKSAFMTFKEFLNEVNHTLMNDFISMPDDEI
                     NRYMKMEKLLENKISRRFKFRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKT
                     TLIKQYDLIRPTRCLIEESQRYLRLEHEDSESFVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVGNRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLIVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKENAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIDELRREDTAIARNIADHIDSFTDYDFAHLLFSDGTV
                     KNAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVKSEVE"
     gene            22122..24254
                     /locus_tag="OUB02_000709"
     CDS             22122..24254
                     /locus_tag="OUB02_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT8884504.1"
                     /translation="MKDKIKGLLTRKKLFRFFKIAVIVVLTTIALLSLLGTVAHASGL
                     VDDTVNADNLYSQYPLENYQLDLYVDNSWGWLPWNWLDGIGKSVQYGLYCITNFIWTI
                     SLYLSNATGYVVQQAYKLDFINDMADSIGKSIQTLAGVTENGFGSTGFYVGFLLLIIL
                     IVGIYVAYTGLIKRETSKALHAVINFVVVFIVSASFIAYAPDYIKKINDFSSDISTAS
                     LDLGTKIMLPDSDSQGKDSVDLIRDSLFSIQVQQPWLLLQFGNSDIEEIGADRVEALV
                     SASPSADDGATREDVVKTEIEDNDNDNLTIPQVINRLGMVFFLLIFNLGITIFVFLLT
                     GMMIFSQILFIIFAMFLPISFLLSMIPSYENMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMIAFLQIVCFAGTYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YMFMRHRARRMEHRIARAVGAGSVAGAVAGTVAGTAMNGGSGKSTRAGTGRKERRENA
                     SFGSRAGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKTKANVSDFKR
                     GIVQGQETRQSVRADKLQQHRQNIADKRMELQKAQEAKQAGRTTDGSATTGATRHHDR
                     PVTASKTDTGKMQEIKRPVTTDKVHTEPTKAQPVKERPLASKSDEPIPQKHGTRLNGV
                     ELNRQTTRKERNIKKATINTTEKKGRKK"
     gene            24251..25258
                     /locus_tag="OUB02_000710"
     CDS             24251..25258
                     /locus_tag="OUB02_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCT8884505.1"
                     /translation="MKLRHIALIGSLFPFLLAIVLFFGVLISAEDDDGGGGSSSWVTG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVPYLLAIIQVESGGTAEDVMQSSESMGLPPNS
                     LDTESSIKQGCKYFASLLSSAENQGIEDINVVVQSYNYGGGYIDYVAKNGKKHSFTLA
                     ENFARDKSGGTKVTYTNPIAVARNGGWRYGYGNMFYVELVSQYLTVSQVSGELAQKIM
                     NEALKYQGWDYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHIPLLQA
                     QAGDLVFFHSTYNAGTYVTHVGIYVGNNQMYHAGDPIGYADLNNSYWQQHLIGAGRIK
                     Q"
     gene            25280..26173
                     /locus_tag="OUB02_000711"
     CDS             25280..26173
                     /locus_tag="OUB02_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT8884506.1"
                     /translation="MFKKKEKPIKEKKICTMKVGTHMKSVIALWVVLIASVSFGVYKN
                     FTAIDQHTTHEIETIQLRLNDTNGIENFVKNFAKSYYTWSNSKEAIEARTQTISAYLT
                     QELQDLNVDTVRTDIPTSSTVTDVLIWDVEQAGENAFTVVYEVDQQIKEGDQTRNVSE
                     TYTVTVHVDADGDMVIIQNPTLAPAMEKSDYEPKVQEADNSVDADTVNDATAFLETFF
                     KLYPTATDKELAYYVEGNALEPINGDYLYSELVNPLFKADGDNVKVSVAVKFIDNQTK
                     ATQVSQYELTLHKDGNWKIIG"
     gene            26316..26630
                     /locus_tag="OUB02_000712"
     CDS             26316..26630
                     /locus_tag="OUB02_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884507.1"
                     /translation="MNKDEILAKAKKENFLGDEREKEIRVKRDAFSLWGLIILGVIIM
                     IIKLVRTQSPADIISLLFCTSGLGFTYEGIKLKNKWHIISGIILLICAGYSFYKFCMG
                     LF"
     gene            26630..26842
                     /locus_tag="OUB02_000713"
     CDS             26630..26842
                     /locus_tag="OUB02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884508.1"
                     /translation="MNDKLILKNNLKKARAEKNLSQSALAEMVGVSRNTISSIETGQF
                     NPTAKLALILCIALDKKFEDLFYFEE"
     gene            26848..27069
                     /locus_tag="OUB02_000714"
     CDS             26848..27069
                     /locus_tag="OUB02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884509.1"
                     /translation="MIGLKKRDMKKILKAGAKASGVPVSELRAEIQATIDEAMNSTDP
                     EVQANFKRYFGNKTPTPEEYIYTITKKTI"
     gene            27255..28247
                     /locus_tag="OUB02_000715"
     CDS             27255..28247
                     /locus_tag="OUB02_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884510.1"
                     /translation="MYEWQKQIQIIVDEIDNCIKNYNDEAPTLRFLARKLGYSEFHTT
                     RKFKEITGIQFRDYLRSRKLAFALKEVRDSKKSLLDIAFDYGFSSNEAFTRAFKGTFG
                     ITPSEYRKNPNPVILRTKITPFDRYYLGLGEIGMIKSNDSVRIYFVTIPAHKFLHIKN
                     YESNGYWDFWQKQNLIPGQDYETICGLLDSIKGKLDDDGGSEVNCGSGQIMAYINDPN
                     GRLCDWGIPRVECWGVRLPLDYNGEIPPQMYMVDIPEAEYIVFEHGAFNYEQENCSVE
                     EKIEQAMKNFDYTSVNYRLDTSPGRIMYFYYNPEKYFKYVRPVKKLEKPQNNQR"
     gene            28322..28681
                     /locus_tag="OUB02_000716"
     CDS             28322..28681
                     /locus_tag="OUB02_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT8884511.1"
                     /translation="MADWLTKRLCTSPIMVEKQSCSLIINPYTRIVTDKNGKTIHLTA
                     KEFDLLHFLSSHKGQVFTKEQIYENVWGYDNAPDASNLSSFIRKLRLKIEPYPDNPRY
                     IITVWGVGYKFSGEEKP"
     gene            complement(28721..29083)
                     /locus_tag="OUB02_000717"
     CDS             complement(28721..29083)
                     /locus_tag="OUB02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884512.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQEAVADLIDITPAHMSNVETGK
                     TKVSLPTLIEIANALSVSVDILLCDNVLASQNIFKGEAKEIFEDCDEYETRFLVDLLK
                     CAKSAMRRDKEVRNQFNR"
     gene            29411..29776
                     /locus_tag="OUB02_000718"
     CDS             29411..29776
                     /locus_tag="OUB02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884513.1"
                     /translation="MGQAEKQKEMFLSICGQHDYNLLTGKEAMTQTDFERITYITMAL
                     GYTSYTQELIGEHLDLACNEAERADREFDILQDYPAYYDDENVYEQRDKWLADFISQV
                     PPAKQDDIRQLIKENTEII"
     gene            30217..30645
                     /locus_tag="OUB02_000719"
     CDS             30217..30645
                     /locus_tag="OUB02_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HCT8884514.1"
                     /translation="MKRPTSPEDVQKVFDCYCKKILKNEAINIQKHYQRMNDLQISFS
                     ELKPEQLAELSTYDDYSTDYDLYKVMGYDVKIKNELLSEALQELSEEERFILLLYGLG
                     FNDGEIADLLKIVRRTVNYKRQRGFEKVRKRMEEKQHEEE"
     gene            30632..30880
                     /locus_tag="OUB02_000720"
     CDS             30632..30880
                     /locus_tag="OUB02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8884515.1"
                     /translation="MKKNKAVHKGNGLLPYPVIVSASQGDILAMNVVLCHYEPYIARL
                     SMRTSIDEYGNPHTSVDEDMRSRLEAKLIEQVLNFKVA"
     gene            31280..31486
                     /locus_tag="OUB02_000721"
     CDS             31280..31486
                     /locus_tag="OUB02_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCT8884516.1"
                     /translation="MTNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWV
                     IVNGNRVQIKRKQFEKIIDTLDEI"
     gene            31566..32762
                     /locus_tag="OUB02_000722"
     CDS             31566..32762
                     /locus_tag="OUB02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8884517.1"
                     /translation="MSNVKRKDNKGRNLRLGESQRKDGRYVYKYTDIYGKPQFKYAWK
                     LVPTDKTPAGKREDLSLREKEKQIKKDLADGINTIGGKMTVCQLYAKKNSQRKNVKRN
                     TEKGRQYLMKILENDPLGARSIDTVKLSDGKEWAMRMYENGFSYKTISNYKRSLKASF
                     YMAIEDDCIRKNPFNFKLSDVLEDNSEPKVILTPEQEEKMLAFMEQDKTYSKYYDEVV
                     ILLETGLRISEFCGLTVHIDMANRIINVDHQLLKDSEIGYYIETPKTKKGERQLPMTE
                     RAYQAFKRVLQNRGKAEPIIIGGYSNFLFLNQKGFPKVAGNYESMLRGLVKKYNKTHK
                     DQLPNITPHSFRHTYCTNMANKGMNPNTLQYVMGHANITMTLGYYAHGTFNSAKAELE
                     RLALAS"
     gene            32939..34429
                     /locus_tag="OUB02_000723"
                     /pseudo
     CDS             32939..34429
                     /locus_tag="OUB02_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34444..35316
                     /locus_tag="OUB02_000724"
     CDS             34444..35316
                     /locus_tag="OUB02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8884518.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            35317..>35671
                     /locus_tag="OUB02_000725"
     CDS             35317..>35671
                     /locus_tag="OUB02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884519.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTV"
BASE COUNT        11963 a         6138 c         7643 g         9927 t
ORIGIN      
        1 taacaggagc aacaggggca acaggagcga caggtccaac aggagcagat ggagcaacag
       61 gtccaacagg agcaacagga aatacaggag cagatggagt agcaggtcca acaggagcaa
      121 caggagcaac aggaaataca ggagcaacag gcccaacagg agcaacagga gcaacaggag
      181 caacaggtgc tagtgcaata ataccttttg catcaggtat accactatca cttacaacta
      241 tagctggagg attagtaggt acacctggat ttgttggatt tggtagttca gctccaggat
      301 taagtatagt tggtggagta atagacctta caaacgcagc aggaacattg actaactttg
      361 cattttcaat gccaagagat ggaacaataa catctatttc agcatacttc agtacaacag
      421 cagcactttc acttgttggt tcaacaatta caattacagc aacactttac caatctactg
      481 caccaaataa ctcatttaca gctgtaccag gagcgacagt tacactagct ccaccactta
      541 caggtatatt atcagttggt tcaatttcta gtggaattgt aacaggatta aatatagcag
      601 caacagcgca aactcgattc ttactagtat ttactgcaac agcttcaggt ctttcattag
      661 ttaatactgt agcaggatat gcaagtgcag gaattgcaat aaattagatt agataagaaa
      721 aggcttctaa tatcgtattg atattaggag cttttttaag aatgattgat gaacatagtt
      781 ttaatattaa ttagtgcgca tattataaaa tactattata taatataaaa taataacatt
      841 aaattggaaa tatagaatta tctaggggtg atactatgaa aaataagatt gaaatatcaa
      901 tgaacaactt tgtaaaaaat tatcataaat caaataatat aactacaaag tggcaagaac
      961 ttttgtttgc ttatgcatct gctgaagatg atatgtttca gaagctaaaa agtgttgtag
     1021 gaacatctca tgctatgcca aaagattttc tggaaaatgg aaaaactata attgcatatt
     1081 ttataccttt tgatgaaagt gtaaataata gtaatattgg agggtttgaa tgttctgaaa
     1141 tatgggcaag agcgtacata gaaactaata tgttgatttc aaatttaaat gatttcttat
     1201 gtgaagaact agagaagata ggggctaatt catatgctat tcctgggtcg tataattttg
     1261 acagtgaaaa attggtaagt gactggtcac aaagacatgt agcattcata gcaggtcttg
     1321 gaacttttgg tttaaataat atgcttatta cagaaaaagg gtgttgtgga agaataggaa
     1381 gcataatcac aagtttagaa ttagagccaa ctaaaagacc agaaaatgaa tactgcttgt
     1441 ataaacataa taatagttgc aaaaaatgtg tcaataaatg cgtgaaagaa gctttaaaag
     1501 aagatggatt taataggaat aagtgttatg aaatgtgtat gataaatgaa aaaaaataca
     1561 cttatatagg tggatgtgat atttgtggaa agtgtatagt tggagttcct tgttcagtaa
     1621 taaacccatc aagataatgg acttaagttg actaataatt ttaaagatag tttataatga
     1681 aaaaatttta gttatcaaaa taaatataat ataaaaacta aataaatgtg tttaaaaaat
     1741 ggataaataa tttgtgttga ttgacaacca gttatactgt tgatagactt atatgtatcg
     1801 ctgtgatata taaggagggt tgagatttta tgtttgattt aatctttatt tttgcatagc
     1861 aagggctatt gcaactatga aatttaatat ataaatagcc ctttgctatt cctaatgaaa
     1921 aattactatt taataggaga gcaaaatgag ctataaaaaa gcaatacata tattaccaga
     1981 agaattactt gaattaatac aggaatatgt agatggtgaa tgtatctata ttcctagaaa
     2041 atctaataat aaaaaagaat ggggaagtaa tacacctact cgtgaagagt tgtcaattag
     2101 aaatatgcag atttacgaag actatcaagt tggatatgat ttgcaatatt tatcggaaaa
     2161 gtattattta tctttgaaaa gtatccaaag aattgtatta caagaaaaaa gaaaagatat
     2221 ctaaggatga gccagtatag ttttataatt atactggctc attttctttt gataaaagtg
     2281 tttgaggtat agtttctttt aagtggatga tataatttaa gtaatttcaa taacttatac
     2341 agtagctaac aaagctggtt gtaaagtatc taataagaag tagtagcttt ttatatgttt
     2401 gaaattaaaa tttattaaaa ttaggaggtc ttgttttgaa aaaaatactt gattatattc
     2461 caccttttca taactttcta ttaatttagt aatcttatag aaaggaaatt gcatttatga
     2521 aaaaaattac ttgggaggtg gaatatatct cccagttacc tgtaattagg tattgtaaaa
     2581 aatgtggaaa aaagactgaa tatatttgtt caggtttatt tagggttaat gctcaacata
     2641 aatatttgga tatatggtta atatacaagt gttcaaattg tgattcaaca tggaactcaa
     2701 ctatatattc tcgtgttaat ccaaaaagct taaatccaga aatactagaa caattccata
     2761 caaatgatta taatcttgtt aagcaatatg ctatgaatat ggaactttta catagaaatg
     2821 gagcagaagt tggtattcca gagtataaaa tagtaggaca agaaattaat attaatgacc
     2881 ttacacaagt tcatattatt agtaaatatc catgtcagtt aaaggtatca acattattac
     2941 gtgagaaact aggtttatct aggaaggtat ttgacgaaat gttggattgt gggattatta
     3001 gaagtaactc aggagttgat ttaaagaaag gtaaattaaa ttatgaaatt atgttagata
     3061 taggcaagga gaagcaaaag tcttcaaatt gattagaata actaacttaa tatattttca
     3121 ggaacatcat ttaaaaaaat agtgacttta aaaaatttaa agccactatt ttttaatatg
     3181 ttttatatga gtatttaaaa taaagtgtgt attcttcaaa tttatagcga taattaaaac
     3241 attactataa aaaggaaggt gcatactttt tatttaacaa aaactagata ttaaaaactt
     3301 ttcgaggaat attttattaa aatccctaaa tatatttaat atatattaaa aaattgtatt
     3361 ataatgaaaa tcaaaataga ttgcaaatcc aaaaagtaat acttaatgtc caaaaagcta
     3421 aaattaaaat ctaaatagct atttactaca gaagtatgtt aaaatatgta aaatgaatag
     3481 aaatttatat atttttatta aaaatatata agaaaggatg tttaagaatt gtgattaata
     3541 taggaatatg tgatgatgaa ctacattatc gaataaaaat aaaagatata ttaagtgaaa
     3601 tattaagttc atatcctata aattataaca tatatgaatt ttcagcagga gaagagttac
     3661 taaataacta tcctaaagat ttagacatat taattatgga catacaaatg aaaacaataa
     3721 atggtatgga tactgctagg aagataagag agtttgatca taagttagaa ataatatttg
     3781 ttacttcatt tgtagagttt atgcaagaag gttatgaggt aaaagcatac agatatatac
     3841 taaagccaat aaataaagaa aaaatttcta aaagtgtatt accttgtata aatgagatga
     3901 tgaaaaagag aaataattac ttaactataa atgtaaaaaa ttatgtagac cgaattaaaa
     3961 tagattctat tacttatata gaaacagata gaccgaatat attgatttat acacatgatg
     4021 atatgtatat tactaaaatg agtatatcta aaatagaaaa gatattaaat gaatatggat
     4081 tttttagatg tcataatagc tatatagtta acttaaaatt agtagaatca atgagtggaa
     4141 gtactgttat agtagatgga aaaagtattc caataagtaa atatagggtt aaaggcttga
     4201 agcttgcgat aactaatata ttaggggaca tagtatgcta acaaatttaa gtgtaggatt
     4261 ttggaatatt attaatttag tttcaggtac tatagattgg attatatttt ttttagtaat
     4321 ccacttagtt ggaaagagga aaatatctaa tataagatat atatttacaa caataattat
     4381 aattatattt atggcataca taaatgtagt taaagtgttt ccaaatatta agattcttct
     4441 atgtatggta ataggaatat cattttatat aacatcatat gaagataaaa tctataaaag
     4501 tataatagtt agtttattgt tttggctagg attaatggtt gctgaaggtt ctgcagtagg
     4561 tctagtagta tttataaata aattaaataa tattaacata acacaacatg aaaacttatt
     4621 tagaatacaa gctattatta tgtcaaaggt atttttattt ataggtttta tattgtttaa
     4681 gtattttaaa ttatctttag attttaaacc taaagatatg atactaatag gaatacctat
     4741 attatctaat atagttagct tattacttat atttgaatat aatttaagag tcaatacaat
     4801 tactacagaa aacttattta tatttatatt tgcaatttta ttgattttat catcaagtat
     4861 aatactttta atagtaatag ggaaaattgt acaagacgat aaattaaatc tagagtatga
     4921 acttataaat gaaagaataa aaacaaacta caggaattat gaaaatataa acgaagttca
     4981 tgatagatta aaatatgttt atcatgactt gaaaaaccat atgatttgta taaaaaatta
     5041 tgacactaaa gaagaaatta tatcatatat aaataactta gagtttcaga taagtgaatt
     5101 tgaacatttt aaaaatactg gaaataaaac attagattta attttagcag aaaaaataag
     5161 tatttgtaaa aagtatgata ttcaaattga agataacata aatatatcta aattaaactt
     5221 tattaaaaat aatgatatat gttcaatatt tgcaaattca ctagataatg caattgaagc
     5281 atgtatggat ataaataatg agatagaaaa aagaatagaa gttaaagcta catatataaa
     5341 taaatttgca ataataaaat ttataaatac taaggtaaat gatattaaac ttattgataa
     5401 atgtattcaa actagtaagg atgataataa gatacatgga ataggacttg ctagtattaa
     5461 atatatagta aataaatatg gtggtgaaac aatagttaat tactcagata atgagtttat
     5521 attaaaaata atgataccaa taaaaagtta ggagagtaca aaatgaaaaa aatagcgtta
     5581 aacttactta aaaacatctc agcattaagt tttgggatag ctgtattatc agcaaacagt
     5641 gcaagttctt gggtagcaca tcaggcaaaa gagccacaag ctttacaaaa actaaaaaaa
     5701 taacattaat aaaaggacct cttcttatta aaggagaggt tttacatgtt gaggaggaaa
     5761 tatgtttaaa agattatcat ataaatttgc caatatatta gttaataatg agattgtaga
     5821 aagtgaagat tttgaaatat atagatatgg atttgaaact ttgatttatt tcatagttaa
     5881 tattttggta gcattattta taggaattat ttttgataga tttatacata cagttatatt
     5941 tttaagttgt tattgcactt taagacagtt cacaggaggt tatcatgcta ggaattataa
     6001 agagtgtaca ttaacatttg cagttattta tttgataact attttttcag ctaataatat
     6061 agatataaat ataagtattt attagtatta ttaatgatta ttagtatact aacaatttac
     6121 aagttagctc ctttagaaca tagaaacaaa cctcttagtg aaagtgagaa aaagcattat
     6181 agaaagactg tgcaaaaaat actattcgta ataatatgtt taattatttt atgtaaaata
     6241 cttaatattt ttcagcaata tatgatttat gcaataattt ctatatattg gattgctata
     6301 ttaatatata ttggaatgaa ggttaatagt gatcagtaag attataaaac attttctaaa
     6361 ttaattaact tcactaatta atattaactt taaaaaaata atgtcaaggt aagtatttga
     6421 acttattaaa tatagttgaa tctattgtgg gttgtatcaa aggacaaata gattcactta
     6481 ataaccagtt tgtaacagag tattgaattg taaaagttaa tacgatagta tgaaaatgta
     6541 atttgaaatt acatttttat aaaaattata ttaactaagg ggagggatac atacaatgaa
     6601 tttgaagaaa aaaattaaaa agttaggagt tacatttgca ataggaacta taattgtatc
     6661 aagtttaggg acatctgtat ttgcagatga ggttagcaca tatgctttaa tatctaaagc
     6721 tttttcaggt aaaaacaata ttatatctag ttcagtatac acttctggaa gtagaacctt
     6781 taaaacgaca gattctcaag ggggattttc ttgtaggcta acagaaagat tctcaggatc
     6841 tagcccagat agaattgtaa aaagacatga agcaaatgct tcttcaaaag ttagtttatc
     6901 aggaggaaca tactatataa ggtatgaagg ttcaacgaat aatgctaaaa catatgcaac
     6961 tttaaattag tattaaaagc atcctataat tatttttata ggatgttttt gtaattgtta
     7021 taatcaaaag actggctagg aggggcataa atatgagtag tattaaggtt atatcagact
     7081 ttataaccaa aataattaga aaaaatattt tatttgcaat aacagttatt attataaatt
     7141 accatctcat acaacgatgg acacatatat ccaatagagg aatttcattt aatgattact
     7201 tatccttgag tgtatatact tgtatgacaa ttagtatttt aaccatgtta aatgtaattt
     7261 tttcaagaaa aaaacaacta aaaatctgat tgtccgctaa aacactgtac ttatcagcac
     7321 attcaaggat tgaaatactt caaattacta cgattttact accattgaat gagccttgat
     7381 acgttagaaa tcccagattt gcaaagatat ggtacagcac atcagtattg aaaaacaaaa
     7441 attgaatatc gcaagtagat tatggaacgc cgatattgac gttccttttc tatttccagc
     7501 cgttcagaaa tggacggctt tttctatatc caaacgaaag gagaatttta aaagctatga
     7561 aaaagatatt gaagcgatta gccacaggct tccttgcctt tgcgactatc gttaccgctt
     7621 tgccgacaac ggcagtccac gcttccgaga agcaatactg gacagaaagt gccgagcgtg
     7681 tcggtatcgt tgaaaaagta atgaatgacg gttctatcgg ttcgacattc aatgagggac
     7741 acatgaccgt tgagggcgag gacgctttct gcgtggatat taacaccaac ttccgcaacg
     7801 gttataagac cagagccgac gcaagcacac gcatgagcta cgaccagatt tcagatgttg
     7861 ccctctcaat cgagtatgtt aatcagtatg tgcagagcca cagcggattg agcagtcagc
     7921 atatctatct tttgaaacag tgcgtcgtat ggcagagatt gagtgttcat cttggctggc
     7981 aatgcgataa cgtcagagct tcctatgatg aaattccaaa agctatacag gacgaggttt
     8041 acgctggggc gaaagccttt gccagcgaga acaaggaacg ctatgaatgt ggcggttaca
     8101 tctactctgg cgagggacag gacattggac agttctgggc gaaacttgcc gttggaaatg
     8161 ccacattgaa aaagacttcc agtaatgcca gcattaccga tgggaacggg ctttactcta
     8221 tcgctggtgc gacttatggt gtctattctg ataaggactg tacgaaacag cttgccaccc
     8281 ttacgactga taacagcgga aatactgata ccgtggaagt aaaggctggc acagtctaca
     8341 tcaaagagct ttctgctcct gctggctata agatggacag cactgtctat tccttaaatg
     8401 tggaagctgg caagacagcg acactcaatg tatccgatac gccaaaggtc acagataccc
     8461 ttatcgagct tttcaagatt gacatggaaa cacagaaaga cgctccacag ggcgacgctt
     8521 ccctagaggg tgcggagttc acatggaaat tctatgcggg acactatacc gctgacaatc
     8581 ttcctagcga gcctacaaag acatgggtga caaagacaat cgctgaaaag gacagcgacg
     8641 gcactatcca ttatgtttcc cgcctttctg atgaatacaa ggtatctggg gacagcttct
     8701 atacacagga cggaaagaac gtgcttcctt taggaacgct gacggttgag gaaacgaaag
     8761 ctcctaacgg ctatcttctt gacggtgcat atatgcagac tgacggaagc gaggaacaga
     8821 ttaaaggaat gtaccttaca cagattaccg aggacggcga cattgccgta ctgtctggaa
     8881 gcaatcagta ccatgtatca gacaaggtta tccgtggcgg tgtaaaaatc cagaaaagag
     8941 atttagaaac cagcgatacc aaaggtcaag gaagtgcgac tttgaaagac gctgaatttg
     9001 aaatcatttc tctgaatgac aacgctgtac ttgtcgaggg gaaactttac agcaagaatg
     9061 aagtggtaaa gacaatcctt accgacattg agggcgtggc ttctacttct gccgacctgt
     9121 taccttacgg aaaataccgt atcgaggaaa gcaaagctcc agagggctat ctgacggacg
     9181 gtgcagaacc gattgaattt gaaatcacag aggacggaaa aatcgttgac cttacaggga
     9241 cagacacatc aatctacaat caagtgaagc gtggcgacct agagggtatc aagattggtg
     9301 ctggcacaca ccagcgtctt gcagaagttc cgtttaggat caccagcaag accacaggtg
     9361 agagccacat tatcgtaact gatgataacg gacagttctc cacttcttct gactgggctt
     9421 cccataagaa caacaccaac gcaggaaaga ccagcgagga cggtatctgg tttggaacaa
     9481 gcgagccaga cgacagcaaa ggtgctttga tttacgatac ctatatcatt gaagaattac
     9541 gctgtgacag caacaagggc tttgagctta tccctgcctt tgaaatcgtg gtgtccagaa
     9601 acaatgtgac cgttgactta gggacactta ctgatgaata cgagaaagaa atctctatcc
     9661 ataccacagc cacaggcaag gacggtgaaa agtctatcgt ggctggcaaa gaggtaacaa
     9721 tcgttgacac ggttacgctt gacgggctgg aaaaaggaac aaaatatcag ttgaagggct
     9781 ggcagatgtt gaaagaggaa aatgcagagc ttcttatcga cggacagcgt gtggaaagtg
     9841 attacacatt cacagccgac agcgaggaaa tgaagattga aatagaatac acgttcaatg
     9901 cttcttctct tggcggtcag aaccttgtaa cttttgagga actttacgac ctttccaatg
     9961 aggacgagcc tgtaaaggtg gctgaacaca aggatattga cgacgaggga cagacggtgc
    10021 ttatcacaga gcgtatcatc tctatccata ccacagcgac cagcgaggac ggaaagaaag
    10081 agattgaagc tggaaaagac gtgacaatca ttgataccgt tactcttgac ggcttggaaa
    10141 tcggcacaaa gtatcagctc aaaggctggc agatgattaa ggacgaaaat gcagaactta
    10201 tcatcaatgg tgagcgtgtc gagaatgact atacctttac tgccgacagt gaaagtatgg
    10261 aagtacagat tgcctttaca ttcgacgctt ccgagcttgg cgggaaagag cttgtgacct
    10321 ttgaggaatt atacgatttg agcaatccag acgaaccgac taaggttaca gagcataagg
    10381 acattgagga cgacgggcag acggtgacaa tcacagaagt accagaaatg ccggaagaac
    10441 caacagagcc ggaacagccg accacaccag atacaccgag caagacaaca gacgctccaa
    10501 agacaggcga taacacaaac attgccttat tcttagggct tcttattctc tctggtgcag
    10561 gcgtggcagg aacatacttc atcaagcgta gacgttcata atcggacggt ctgcttatga
    10621 tgatgaagaa atcacgttcc ccgccgatac atggaaacgg cggagcttcc ctttattctg
    10681 gcttgtccgc cacccacctg taagcacaca gacagtcaag ggtgctttta agcatttacc
    10741 tgcccttgac ggtctggggg cttacgggta tcacaccacc agccagaata aaagcattga
    10801 taatcaacag attttataga aatgaggtat gaacacatga aattgaaata tatcgtgcct
    10861 gacatggcac agacttttgg taacttggag tttgcaggag aaaatgaggt agagcaacag
    10921 cgtgtcaatg gtcgctttgt tcccgtgaca agaagctata acctgtattc cgacattcag
    10981 agagccgacg acattatcgt ggtgcttccc gcaaaagctg gggagaagcg tttcaagtat
    11041 gagcaaaaag taaagctcgt caatcctaag attaccgccg agggaaagaa cgtgcgaggt
    11101 cgtggctaca ccaactatat tttaaacgct gacgatatgc ttccagtcga ggaaagcaag
    11161 taaaggggga taacagatta tgagattagc gaatggaatt gtattagata aggaaaagac
    11221 tttcggtgtt ttgaaattct ctgcccttag acatgaggtg cgtgtagaga atgaggacgg
    11281 gacgaccact gaggaaatca agcgtagaac ctatgatttg aagtgcagtc agcaggaacg
    11341 cacgatacag gtatctatcc cagcggaaat ccctgtcaag gactatccgt acaatgccga
    11401 ggtggagctt atcaatcctg ttgccgacac cgtggcaaac gccaactacc gaggggcaga
    11461 tgtggaatgg tacatcaagg cagacgacat tgtactgaaa aataaaggcg gtcagcccgc
    11521 aggcaatccg cagaaccacg ctccgcaggg acagcagaaa aaataaacgg ataaaagcta
    11581 atatgtattg cctgcccttt caagctccta tataatgaga acgaagcaat atatgagaac
    11641 gaagcaatat aggagtgatg ataattagtg aaaactttcg caaaaatcat aaaaaagatg
    11701 tattggctgg gattgatagg ttttcttgga acatttcttg atattccagt gttgcagtta
    11761 ttctatctgt tttttctact ggcgattgta gattttgttc tatcttttat cattgtgata
    11821 cagacagaaa gcacagatga aacagcaatc gccttgaaat tcttgtctca aaatataggt
    11881 atgctgattg gaatacctgt gatatatgta cgaaacttat tttttctgcc taatatccgt
    11941 aattataaac cacagacttt atatagtctc ccatttacgg ggtgttggac tgtggcaaac
    12001 ggcggaataa atagagaaac ttcccattct tggtctatct gcaatcaacg atatgcgtat
    12061 gattttttcg ttgaggaaaa tggaaaaaca tttcatggga acgggaaagc cgtatctgat
    12121 tatttctgct acggaaaacc tgttttatct cctgctgacg gcatagtggt tgaaatcaaa
    12181 aatctttttg aagatactcc tatcccagag gagcaagagg tattatgtag tgcttctgac
    12241 gtaagaggaa attatatcgt tattcagcat acaaagcatg aatatagtac catagcacat
    12301 atcaaaaaag acagcttttg tgtaaatgta ggcgataaag tatgtcgggg acaacagata
    12361 gcctgctgtg gcaatagcgg taacacaagc gaaccgcata tacattttca agtacagcag
    12421 ggaaagagtt ttcttttttc cgcaagcctg ccaatatggt tcacaaagat aatcaatcac
    12481 acaatctcaa aagcagactc acatatcagt aggggacaaa tagtagaaaa tcagaaataa
    12541 gtaatcaagc ggttagtctt tacgattgac cgctttttcc atatccagaa aggggtgttt
    12601 atgaacattt tgaaatataa gggaaagcgt atcagagcca gcgacaagaa acttgtgtac
    12661 cgcttctgtg cgggtacgct cctgctcctg ttcgtggcgg ttctcctgct attgaacatg
    12721 ggacagctca tgcgtaccga ttgggagcat ttcagtttac tggataatgg cataacgctt
    12781 tccgcataca acttcataac catagggata gcgactggtg tttgtgcatt ggtcgctttt
    12841 ctttattacc gcttcttcca tgacagcttc aagaagctat tgcaccgtca aaagctctcc
    12901 cgcatgatac tggagaataa atggtatgaa gcccagaccg tacaggatag cggttttttc
    12961 actgacctgc agagcagatc aagggaaaaa atcgtctggt ttcctaaaat ctattatcaa
    13021 atggacacgg gactgctcca tatccgctgt gaaatctctt tgggaaagta tcaagaccag
    13081 cttttgcgac tggaagataa gctggaaagc gggctttact gcgagctgac cgacaagacg
    13141 ctccatgacg gctatatcga gtacaccctg ctctatgaca tgatagcgaa ccgtatcacg
    13201 attgacgaag taaaggctga aaacgggggc ttgcgtctga tgaagaacct tacatgggaa
    13261 tacgacgcac tccctcacgc cctaatctgt ggcgggactg gcggtggaaa gacctatttt
    13321 ctgctgacaa tcattgaagc cctcttgcag accaacgccc agcttttcat cttagaccct
    13381 aagaacgctg accttgcgga cttgggtacg gtcatggata acgtgtacca caccaaagaa
    13441 gatatgattg aatgtgtcaa tgcgttctat gagggcatgg tagcaaggag cgaggaaatg
    13501 aaacgccacc cagactacaa gacgggcgag aactatgcct atctgggctt accaccctgc
    13561 ttccttatct ttgatgagta tgtggcgttc ttggaaatgc tggggacaaa ggaaagcatg
    13621 agccttttaa gccagctaaa gaaaatcgtc atgcttggca gacaggcggg ctatttcctc
    13681 attgtcgcct gccagcgtcc cgacgcaaag tatttcagcg acggtatcag agataacttc
    13741 aacttccgtg tgggcttggg acgtatcagc gagcttggct acggtatgct tttcggaagt
    13801 gacgtaaaga aacagttctt ccagaagcgt atcaaagggc gtggctattg tgacgtaggt
    13861 acgagcgtca tatcagagtt ttacgctccc cttgtaccaa aggggcatga ttttttacag
    13921 actatcggct ctcttgcact ggcaaggcaa gccgtaccag agaaacaaaa ggagattgaa
    13981 tgattatggg atatttggaa atccattatg aaccagaatg taccgacagt gtacttacct
    14041 gtatcggatt ggggtatgga aaattcctgt ccgatttggc ttttacggca gacagtgaat
    14101 ataaacagga cgacgactat ccagaaacac ttttccacaa aagaatgtcg gaactgctgg
    14161 aagatttggc agaggattat ctggaaatgc agttgctgtt ttccgtggag cttcccgcac
    14221 acatggcgaa cctgctgggc tgtcttttcc gctatacgtt tcttgtcatg gacagggagc
    14281 atttcagaca gatatgccgt gaatacgaga ttgacaagga tatagccaga aaatgccttt
    14341 caagagatac tgactgtatc gtagtttata cgggcatgac aaggataggc tgaaccactg
    14401 acggctggaa cggtcagcga agcgccccag accgtgtaag ccgtcctgtg gagcagggct
    14461 tgccctgctc cacgaaagaa gcccctcggt atctaacaga ggggcacaat tccgctggaa
    14521 actacggtca aaaagtctgg aaatcctgct tagtataagg gttttctctc actcatgact
    14581 gactgtgaca atcggcggaa aatatcacag atttctataa atacacgatt gggggtatgc
    14641 aggctgaatg aaagcacctt tataatcgcc ttgaaagaga aacgcttatc ttacggcgtg
    14701 tcccagacaa ggcttgcaat catggctggt atcagccgtg aacacctaaa ccgtatcgaa
    14761 gctggaaagg taacgctcac ggacgatatg caggacaagc tcatggaagc tgttgagaaa
    14821 ttcaatcccg acgctcccat gttcctgctg ttcgactatg tgcgtatccg tttccccacg
    14881 ctggatatac agcacattat caaagatata ttgaagctca atatcgacta tatgctccat
    14941 gaggactatg gacactacaa atacaccgag cattactatc tgggtgatgt gttcgtctac
    15001 acctcgcagg acgaggaaaa aggcacgctt ctggagctga aaggaaaagg ctgtcgccag
    15061 tttgaaagct atctgctggc acaggagaga agctggtatg acttcctcat ggacgctctg
    15121 gttgagggcg gtgtgatgaa acgtcttgac cttgccatta acgacagggc gggcatactg
    15181 gatattccag atctgaccgc caaatgcaac cgtgaggaat gtgtttccct gttccgttct
    15241 ttcaagtcct atgcttccgg cgagctggtg aagcacaacg agcaggataa ggcgggcatg
    15301 ggacacacgc tgtatatcgg ctcactgaaa agcgaggtgt acttctgctg ttacgagaag
    15361 aactacgaac agtacgcaaa gctgggcgtg ccgattgagg aagcacccat aaagaaccgc
    15421 ttcgagatac gactgaaaga tgaaagagct tattatgccg tccgtgaact gctgacccat
    15481 tatgacgcag aacagacggc attttctatc atcaatcact atatccgctt cgtggacaga
    15541 gaaccggaga agcgaaagac ggactggaag ctaaatgacc gctgggcgtg gtttatcggg
    15601 aaagaccgcc cgcctatcaa gctgacaact gacccagagc cttacacatt ggaaaggacg
    15661 ctgggctgga taagcaggca ggtcgctcct acgctgaaaa tgctgaaaaa gatagacgct
    15721 ggcaacagca caagctatct gaaagagatt gaagataacg caaagctgac agaaaagcac
    15781 ttgcagataa tcagacagca gacagcggac acagaggaac ttatcacaga gtaaaggaga
    15841 attgagatta tggcagaact tgtcaaggac ttggtgctgg tatcgctggg attgggtatc
    15901 ggcgttgtcc ttatgtgtat cgcacaggtc agcaaaatgg ctgacgaaca aatggaagaa
    15961 atcaagaaag aaaggattga taaagaatga atttcggaca gaacttatac aactggtttt
    16021 taagcaacgc ccagagcttg gtgcttatgg caatcgtggt aatcggtatc tacttaggat
    16081 ttaaaagaga gttttctaag ttaatcggct tccttgtgat tgccttaatc gctgtggggc
    16141 tggtattcaa tgccggcggt gtcaaggacg tgctgttaga gctgttcaac cgtatcattg
    16201 gagcgtaaca atcaatcgtt tgcggtcacg caaacttacc tgtcccccta tgggagttcg
    16261 ggcatagggg ggggggaatg aaagggggta acggaatggt acacaaataa aagatagtca
    16321 cgctatgaat ttcattgaat gactaatttt taactgatat aatgtgagta tagaaatcga
    16381 caaattggaa tttgaaaagg agatatactg aattggatac taaacggtta gcaaaatttc
    16441 ttataattac atttgttatt acagggatag cctggtctgg acttgctgtg ttaacaagtt
    16501 taaatattat cccattttcg catcctttag gcactatttt acatattata ggaggattcg
    16561 gaccgacaat agcaactttc tttgtcttag aagaaaaaaa cacggttaaa tctatattac
    16621 attttatttt tggctacaga aagaaatcat tgattttttt gtttttattt tctatttttg
    16681 aaatactgac aataggttta tcgtcaaggg aatttaattc cgcattacca tggtatttga
    16741 tgcctttaat ctttcttcaa gcaacattta tatatggcgg cgaagaagaa ttaggttgga
    16801 gaggtgttat gcagccttta ttagaggaaa aattgaattt ccctatagcc acaatcatta
    16861 cgggtgttgt gtggggaatt tggcatattc ccctttggtt tgtaaatggc tcatcgcaac
    16921 aaaatatgcc atttctcttt ttcttagcgt tagctgttat actgagcttt tggctggcga
    16981 caatttacaa aaaaacgaaa tgtgtttttg cttgtagtgt ttttcatgga ttgacaaata
    17041 ctcttttgtc tgtatttata attaaagtga atgttctatt ggtaatagga ctgattgcta
    17101 tgcttgtata ttcaatatat ttatggtatt gtggagaagc aaaacaataa attcttattt
    17161 agtgatttgt ttaataaata gtaagttgta gattggagtt agttatgaaa aatgtaatat
    17221 atatattaat tcaatgcact tggggattgc cacaaacatt gttaggattt atagtatttt
    17281 taactaatat aaaaaataaa cattattttt atcacggtgc aattattact gaaagaaatg
    17341 ttccttcaag tgtatctttg ggaatgttcg tctttacaac aactaatccg atgaaagata
    17401 aaagaacaaa aaataaaata ccagatgaag aattaagtaa aaggctttta gttcacgaat
    17461 atggtcatac aattcaatcc cttatttttg gaccattata tttaatagta atggggattc
    17521 catcaacact atggggattt ttgccatatt ttcaaaataa aagaaacaac ggagtatctt
    17581 actttagctt ttttacagaa aagcttgcta attatttagg tgaaaaagtt acaaaagaaa
    17641 aatcaatgga aaatgctatt atataaataa gaattgatgg aggtaaaata cggtgtttca
    17701 agaattagga attagtgcag gaacagcaat tattatattg attacattat attttattat
    17761 taaatgggct gtaaagaatg gaataaagga agcctatcaa gagataaaga aaaagtcacc
    17821 agaagattta cgatttgaaa gaagagaaaa ggatattaat aaatcctaat ttaacaattt
    17881 catatcccac atataaagcg gttactcctt tggagatcaa ccgctttttc tatactcaaa
    17941 tttaagattg gagtgattga ttgaaagaca tttttaccga tatgcaggca aaaatcgggt
    18001 gtccgtatct ctccgacctg ccctattaca agcgtgcggt ctggtttgag atgaaacgac
    18061 tttgcctgtc cgattaccct aagaaacagt tagaagattt ttcacgctat gtattcggtg
    18121 tgccttacgc cgtcatacag gaagcgttga cgagaaagga cgtgatgaaa catggaagaa
    18181 atgcgtgtgc agattgaagc aagagaaccg acagacggag aaatcagagc cttttggttc
    18241 accctgccta tcgacacgga acaggtggaa gaactgctgg ggattgatat agatacagac
    18301 tattactata tcacgggcaa ggagcttccc tttgctgatg aagtgcagga agatactacc
    18361 gtcgaaaagc tcaatgacct gtaccatacc tacgaaaatc taccctctga tttgaaagag
    18421 gactatacgg agctgatgtg ctatttctct gaccttgacg aactgcaccg atacagaaac
    18481 gacatcattc attactcatg gtgtaagaac atgacggacg ttgcccgcca tatactgaac
    18541 aacgacccag actttacttc ccttagtgag agcgtcaccc gctatttcga ttttgaagcc
    18601 tacgggcagt accttgacga caacggacgt tttgtggaaa ccgaccacgg tatctatgag
    18661 cttccgtaga aaagaggtgt gtgcctatgg ttgacgacat gagggtgtat atcgcgaatc
    18721 ttggcaagta caacgagggc gaacttgtcg gggattggtt ctctttccct atcgacgagg
    18781 aagatgttgc ggagcgtatc gggctgaaca gctattatga ggaatacgct gtccatgata
    18841 ccgacaactt tcctattgaa atcggggagt atatctccat tgaagaactc aacgagatgt
    18901 atgacatgat ttgtgagctt cccgactata tcaccgacgc tctggacgag tttgtttccc
    18961 actatggaag tctggaagag gtctacgaac acaaggacga catttatttc tatccagact
    19021 gtgacgacat gaccgacatt gcttattact tcattgacga gttacaggta ttaggtgaaa
    19081 ttccgctacc gttacaaaac tatatcgact atgaagcata cggcagggac ttatccatag
    19141 aggggacatt tattgaaaca agccgaggga tatgcgagat accatattag agctggcaga
    19201 tcagcgacag gaaatgttgc tactgacagc tttttctatc aaggacagtc tgaaaaacgg
    19261 ctgtcctttt ccattacaac gaaaggagct gaaacgaatt gaagaagatt aagagctaca
    19321 cgggtatctg gaacgtggag aaagtcttat atgcaatcaa tgactttact ctcccgtttc
    19381 ccgttacctt tacccagata acgtggtttg tcattacgga gtttgccgtc attctcttag
    19441 gggacttacc gccgttatcg cttatcgagg gggcattttt aaaatacttc ggtattcccg
    19501 ttgccctcac atggttcatg agccaaaaga cctttgacgg caagaagcct tacagcttct
    19561 tgaaatcgca gattacgttt gccctgcgcc caaaagtgac ctatgcagga aaagccgtga
    19621 agctggaaaa ggaagttttc agcgaacccg tcacggcagt taggagtgtg atgtatgttc
    19681 ccgattaagt atatcgacaa caaccttgtc tggaataagg acaatgaggt attcgcctat
    19741 tatgagctga taccatacaa ttacagcttt ctatcggctg aacagaaatt tatcgtccat
    19801 gacagcttcc gtcagcttat cgcacagtcc cgtgagggaa agatacacgc cttgcagata
    19861 gcgacggaaa gctccgtaag aagcatacag gagcagtcaa aaagattagt gacggggcga
    19921 cttcgtgatg tggcaatcca gaagataaac gaacagacgg aagcccttgt gtccatgatt
    19981 ggcgacaatc aagtggacta ccgctttttt atcggcttca agctcattgt gacagaggaa
    20041 aaagtcagtc tggatagcgt gaagaaatcg gcatttatga cgtttaaaga gttcttgaat
    20101 gaggtcaacc acacgctgat gaatgacttt atctccatgc cagatgatga aatcaaccgt
    20161 tacatgaaaa tggagaaact gctggaaaat aaaatctccc gccgtttcaa gttccgacgc
    20221 ttggacaaga acgacttcgg gtatcttatc gagcatatct acggcaggga cggcgtggcg
    20281 tatgaggact acgagtattc gcttccaaag aagaagctga aaaagacaac gcttatcaag
    20341 cagtatgacc ttatccgccc gacccgctgt ctgatagagg aaagccagcg atatttgcga
    20401 ctggaacatg aggacagcga gagcttcgtg tcctacttta ccgtcaatgc cattgtgggc
    20461 gagctggatt ttccgtcgtc ggaaatcttc tatttccagc aacaacagtt cacctttccc
    20521 gtggacacct ctatgaatgt tgaaatcgtc ggcaaccgca aggcactttc tactgtgaga
    20581 aataaaaaga aagagctgaa agacttggac aatcacgcct atcagtctgg aagtgaaaca
    20641 agttcaaatg tggtggacgc actggacagc gtggacgagc tggaaaccga cttagaccag
    20701 agcaaggaaa gtatgtataa gctctcctat gtgataaggg tatcggctcc cgacctcgac
    20761 gagctgaaaa gacgctgtga tgaagtcaag gatttttatg acgacctcaa tgtaaaatta
    20821 gtccgtcccg ccggggatat gctggggctt cactctgaat ttctcccagc cagcaagcgt
    20881 tatatcaatg attatgtgca gtatgtgaaa tccgactttc tggctgggct gggcttcggg
    20941 gcgacacaac agcttggcga gaatacgggt atctatatcg gttattccgt ggacacggga
    21001 agaaacgtct atttacagcc gtcccttgct tcgcagggta tcaaggggac agtcaccaac
    21061 gctttagcgt ctgcctttgt gggaagtctt ggcggtggga aatcattctg taacaatctg
    21121 attgtctatt attccgtcct atttggggga caggcggtca ttctcgaccc taagagcgag
    21181 cgtggaaact ggaaagaaac actcccagaa atcgcccatg aaatcaatat cgtcaatctt
    21241 accagcgata aggaaaatgc agggcttctt gacccattcg tgattatgaa gaatgtaaaa
    21301 gacgctgaaa gtctggcgat agacattctc acattcctta cggggatttc ctctagggac
    21361 ggtgagaagt tccctgtcct tagaaaagcg gtgcgagccg tgacccagag cgatcagcga
    21421 ggcttgctcc atgtgataga cgagctacgc agagaggaca cggcgattgc ccgcaacatt
    21481 gccgaccata tcgacagctt tacggactat gactttgcac atctgctgtt ctccgacgga
    21541 acagttaaga acgctatcag tttggacaac cagctcaata tcatacaggt ggcagatttg
    21601 gtactgcccg acaaggacac gacctttgag gaatacacga ccattgaact gctgtctgtg
    21661 gctatgctga ttgtcatttc cacgtttgcc ttagacttca ttcattcaga ccgcagtatc
    21721 tttaaaatcg ttgaccttga tgaagcgtgg gctttcctca atgtggcaca gggcgaaacc
    21781 ctctctaaca agctggtgcg tgcggggcga gctatgcagg cgggcgtgta ctttgtcacg
    21841 caatcctctg gggacgtgtc caaagagagc ctaaagaaca atatcggctt gaagtttgcc
    21901 tttagaagta ccgacatcaa tgagataaaa cagacccttg aatttttcgg tatcgacaag
    21961 gacgacgaga acaaccagaa aaggcttcgt gacttggaga acggacaatg cctgcttcaa
    22021 gatttgtacg ggcgtgtggg cgtggtgcag atacaccctg tctttgagga attattgcac
    22081 gcctttgata ccagaccgcc cgtgaaaagc gaggtggagt gatgaaagat aagataaagg
    22141 ggcttctgac aagaaagaag ctctttcgct ttttcaagat agctgtgatt gtggtactga
    22201 cgaccattgc cctgctctcc ttattgggga cggtggcaca cgcttctggg ctggtagatg
    22261 ataccgttaa tgcggacaac ctgtattcgc aatacccttt ggagaattac cagcttgacc
    22321 tctatgtgga caatagctgg ggctggcttc cgtggaactg gctggacggt atcggtaaat
    22381 ccgtacaata cgggctttac tgtatcacca actttatctg gactatcagt ctgtatctaa
    22441 gtaacgccac ggggtacgtc gtccagcagg cgtataagct ggacttcatc aacgacatgg
    22501 cagacagtat cgggaaaagc atacagacct tagctggcgt aacggaaaac gggttcggaa
    22561 gtacgggctt ctatgtaggc ttcctgcttc tcattatcct tatcgtgggt atctatgttg
    22621 cctacacggg actaataaag agagaaacca gcaaggctct ccacgccgtt atcaacttcg
    22681 ttgtggtatt tatcgtgtcc gcttccttta tcgcttacgc tcccgactat atcaagaaaa
    22741 tcaatgactt ttcttcggac atttccacgg cttcgctgga tttggggaca aaaatcatgc
    22801 ttcccgacag cgacagtcaa ggaaaagaca gtgtagattt gataagggac agcctgttct
    22861 ccatacaggt acaacagccg tggttacttt tgcagtttgg aaacagcgat atagaggaaa
    22921 tcggggctga ccgtgtggaa gctctggtat ctgctagtcc ctctgccgac gacggggcga
    22981 cccgtgagga cgtggtgaaa acagaaatcg aggataacga caatgacaat ctgacgatac
    23041 cgcaggtcat taaccgtctg ggaatggtgt tcttcctgct catcttcaat ctggggataa
    23101 cgatattcgt attcctgctt acgggcatga tgatattttc ccagatacta tttatcatct
    23161 tcgctatgtt cctgcctatc agctttctac tttctatgat acccagctat gagaacatgg
    23221 cgaaacaggc aatcgtgcgg gtgttcaata cgattatgac aagggcggga ataacgctta
    23281 ttgtcacggt agctttcagt atttccagta tgttctacaa catatccacc gattacccgt
    23341 tctttatgat tgcgttcttg cagatagtat gttttgcggg tacctacatg aagctgggcg
    23401 accttatgag tatgttctct ctcaacgctg gggacagcca gagcatggga cgcaggatat
    23461 tccgtcgtcc gtatatgttc atgcgacaca gggctaggcg tatggaacac cgtatcgcaa
    23521 gggctgtcgg tgctggaagt gtcgctggtg cggtggctgg tacagtagct ggaacagcca
    23581 tgaatggcgg ttctgggaaa tctaccagag ctggtacggg cagaaaagag cgtcgagaaa
    23641 atgcgtcttt tggaagccga gctggttcag ccgtgggtgc ggtgcttgat acgaaaaata
    23701 aggttaagga caaggcaacg gctgtcaagg agaatatcaa ggatatgccg acccagactg
    23761 cctatgccgt acactccgca aaggaaaaga caaaagcgaa tgtgtccgac ttcaagcgtg
    23821 gcattgtgca gggacaggaa accagacaga gcgtgcgtgc cgataagctc caacagcata
    23881 gacagaatat cgctgataag cgtatggagc ttcaaaaggc acaggaagca aagcaggctg
    23941 gaagaacaac ggacggatca gcgacaacag gagctacccg tcaccatgat agacctgtca
    24001 ccgcctctaa gacagacaca ggaaaaatgc aggaaatcaa gcgtcctgtc actaccgaca
    24061 aggtacatac cgagccgacg aaagcacagc ctgtcaagga gcgtccgctt gcttcaaaat
    24121 ctgatgaacc tataccacag aaacacggga caaggcttaa tggtgtggag ctgaaccgac
    24181 agaccaccag aaaagagcga aacattaaga aagcgaccat aaacaccacg gaaaagaaag
    24241 gacgtaagaa atgaagctaa gacatatcgc tcttatcggt agcctgtttc cctttcttct
    24301 tgcgatagtg cttttctttg gtgtgcttat cagtgccgag gacgacgacg ggggcggtgg
    24361 ttcttcctca tgggtaacag gcatgaacct gtccgcagag gtgttgaaac atcagcctat
    24421 ggtagagaaa tacgctaaag agtacggtat atccgagtac gtcccctatc tgctggcaat
    24481 catacaggtg gaaagtggcg ggacagccga ggacgttatg cagagttccg agagcatggg
    24541 cttaccgcct aattcccttg atacagaaag ctcaatcaag caggggtgca agtattttgc
    24601 gtccctgctt tcttcagcag aaaatcaagg catagaggat ataaacgtcg tggtgcagtc
    24661 ctataactat ggcggtggct atatcgacta tgtggctaaa aacgggaaaa agcacagctt
    24721 cacgctggca gagaatttcg cccgtgacaa gtcgggtgga acaaaagtca cttacacgaa
    24781 ccctatcgct gtcgcccgca atggtggctg gcgttacggg tacgggaata tgttctacgt
    24841 ggagcttgtc agccagtatc tcacggtcag tcaagtatcg ggcgagctgg cacagaagat
    24901 aatgaatgaa gctctgaaat atcaaggctg ggactatgtg tacggcggga gcaaccctaa
    24961 cacttctttt gactgttcgg ggctggttca atggtgctac ggcaaggctg gcatttcctt
    25021 accgagaacg gcacaggcac agtatgacgc tacccagcat atcccgcttt tacaggcaca
    25081 ggctggggat ttggtgtttt tccacagtac ctacaatgca gggacgtatg taacccacgt
    25141 cggtatctat gtgggaaata accagatgta ccacgctggc gacccgatag gctacgctga
    25201 tttgaacaat agctactggc aacagcacct tatcggggct ggcagaatta agcaatagaa
    25261 agaaaggaat ggaaatatca tgttcaagaa gaaagaaaaa ccgataaagg aaaagaaaat
    25321 ctgcactatg aaagtgggga cgcatatgaa gtccgtcatt gccctctggg tggtgcttat
    25381 cgcaagcgtg agctttggtg tgtataagaa ttttacggct atcgaccaac acaccacgca
    25441 tgagatagaa accatacagc tccgcttgaa tgacaccaac ggcatagaaa attttgtgaa
    25501 gaactttgcg aaatcctact atacatggag caacagcaag gaagcaatcg aagcaaggac
    25561 acagactatc agtgcctatc tgacacagga attgcaggat ttgaatgttg atacggtgag
    25621 aacggatata ccgaccagct ccacagtcac ggatgtactt atctgggacg tggaacaggc
    25681 tggggagaac gcttttactg ttgtctacga agtagatcag cagataaaag agggcgacca
    25741 gacaaggaat gtatcagaaa cctataccgt gaccgtccat gtagacgctg acggggatat
    25801 ggtgattatc cagaacccga cccttgctcc tgctatggaa aaatctgatt atgaaccaaa
    25861 ggtgcaggaa gcggacaaca gcgtagacgc tgacacagta aatgacgcta cggcatttct
    25921 ggaaacattc ttcaagctgt acccgaccgc aacggacaag gagcttgcct actatgtaga
    25981 gggaaacgcc cttgaaccga taaacgggga ctatctttac tccgagcttg tcaatccttt
    26041 atttaaggct gacggtgaca atgtgaaagt gagcgttgcg gtgaagttca ttgacaacca
    26101 gacaaaggct acacaggtat cgcaatatga gctgacgctt cataaagatg gcaactggaa
    26161 gataatagga taacgattga aggcgtgtat ttctcatatg agatttgcac gctttaaaaa
    26221 tgacaacaaa actttgcaaa ctctattgac aagtaaactt ggtaatgata tatttatgtt
    26281 gacaataaaa cttgtcagac aaggaggata tatcaatgaa taaagatgaa attttagcta
    26341 aagcgaaaaa ggaaaatttc ttaggtgatg aacgtgaaaa agaaatacgt gtaaaaagag
    26401 atgctttctc actgtgggga ttgattattt tgggagtgat tataatgata attaaactag
    26461 ttcgtacaca atcaccagca gatataatat cattgctgtt ttgcacatct ggtttaggtt
    26521 tcacttatga aggtattaaa ttaaagaata aatggcatat catttctgga attatacttt
    26581 taatatgtgc tggctattca ttttataaat tttgtatggg gttattttaa tgaatgataa
    26641 attgatttta aaaaataatc taaaaaaagc aagagcagag aaaaatttat ctcaatcagc
    26701 attagcggag atggtgggag tttctcgtaa tacaattagt tccattgaaa caggacaatt
    26761 caatccgact gctaaacttg cacttatcct gtgtattgca ctcgataaaa aatttgaaga
    26821 tttattttat tttgaggagt gaataaaatg attggactaa aaaaacgaga tatgaaaaaa
    26881 atcttaaagg ctggtgcaaa ggcaagcggt gtccctgtat cggaactaag ggcagaaata
    26941 caggctacca ttgacgaagc tatgaacagt acagacccag aggtacaggc gaacttcaag
    27001 cgatattttg gaaacaaaac gcctacacca gaggaatata tctatacgat tacaaaaaag
    27061 acgatttaac aaaggtactt ttatatttat ctatggtatg gtgagctgtg cgaataaaac
    27121 caaaaatgct tgggggtgta tcttcgcatt tttgtttctt tggaatgcgt tctaaaaatc
    27181 gcaagtcaaa tcaagaaatt tgtcccttat atccttataa ttaaattgga gtggttgaat
    27241 aggagtggaa ttgaatgtat gaatggcaaa agcaaataca aataattgta gatgaaatcg
    27301 acaactgtat taaaaattat aatgatgaag cccccacgct tcgttttctt gcccgcaagt
    27361 tagggtactc tgaatttcat acaaccagaa aatttaaaga gattacagga atacagttta
    27421 gagattattt acgaagcagg aaacttgcct ttgcattaaa agaagttcgt gatagcaaaa
    27481 aaagtttact ggatattgca tttgattatg gattttcttc aaatgaggct tttaccagag
    27541 ccttcaaagg aacatttggt ataactccga gtgaatatcg aaaaaatcct aaccctgtca
    27601 ttctccgcac taagataact ccttttgacc gctactactt aggattagga gagattggaa
    27661 tgataaagtc aaatgacagc gtaagaattt attttgtgac tataccagct cacaaatttt
    27721 tacacatcaa aaattatgaa agcaacggtt attgggattt ttggcagaag caaaatctta
    27781 ttccgggaca agattatgaa acaatatgtg gtttattaga cagtatcaaa ggcaaattag
    27841 atgatgatgg cggaagtgaa gtcaactgtg gtagcggtca aattatggca tatataaacg
    27901 accctaatgg cagactttgt gactggggaa ttccacgtgt ggaatgttgg ggagtaaggc
    27961 tacctttaga ttataatggt gaaataccgc ctcaaatgta tatggttgat attccagaag
    28021 cggagtatat tgtctttgag catggagctt tcaattatga gcaggaaaat tgtagtgtag
    28081 aagaaaaaat tgaacaggca atgaagaatt tcgattatac tagcgtcaat tatcgtcttg
    28141 ataccagccc tggaagaata atgtattttt attacaaccc tgaaaaatat tttaaatatg
    28201 tcagaccagt taaaaaatta gagaaacctc aaaataatca acgatagttg aaagttggtg
    28261 atattatggg aaaatattga ttgcagaatt ttcggataaa gaagaagctc tgttttacca
    28321 aatggcagat tggcttacta agcgattatg cacttctcct attatggttg agaagcaatc
    28381 atgtagttta atcataaatc catatacacg tattgtcact gacaaaaatg gtaaaaccat
    28441 acatttaact gctaaagagt ttgacctatt gcattttctt tccagccaca aagggcaggt
    28501 attcacaaaa gaacagattt acgaaaatgt atggggatat gataatgcac cagacgcaag
    28561 caacctttcc tcattcatca gaaagttaag actgaaaata gaaccatacc cagacaatcc
    28621 acgatatatc atcactgtct ggggcgtggg atataaattt agcggagaag aaaaaccgta
    28681 gctgtatttc acaattcatt gtgatctgca cgctacggtt ttatctattg aactgatttc
    28741 ttacttcttt gtccctacgc atggctgatt ttgcacattt cagcaggtca actaaaaatc
    28801 gggtttcgta ttcgtcgcaa tcctcaaaaa tctcttttgc ttctcccttg aaaatattct
    28861 gtgaagcaag tacattatca cataggagaa tatcaactga tacggaaaga gcattggcaa
    28921 tctcaatcag tgtcggcagg ctgactttcg ttttccctgt ttccacattg ctcatgtggg
    28981 ctggggtaat atctataagg tctgcgacgg cttcctgtgt tatgcctttc ttaattcgtg
    29041 ctatcttgat acgctgtccg attgctttat agtctatttc cacagtttcc ctctcctttc
    29101 ttgcttttca ttattgtaac tattcacaga gtgtaatata atagtctgta tagatgaaac
    29161 atttactcgc tatatttttg gcggattttt ttgaaatata tcatcaattt tgagaaaaga
    29221 ggtgttctga ccatatgatg aaattcagta aaagggacag taggaaaatg ataaaagaaa
    29281 tggctcgtct gcacggacta tctgtttcag aagtccgtga gcagatacag gggaaaatca
    29341 tagacgctat gaacagtgac gacctagaac agcaggcagt taaaatttta aggatttagg
    29401 agtggctatt atgggacaag cggagaagca aaaggaaatg tttctgtcta tatgcggaca
    29461 gcatgactac aacttactga cgggcaagga agccatgaca cagacagatt ttgaacgtat
    29521 cacttatatt actatggctc tgggctatac ttcctatacg caggaattga ttggtgaaca
    29581 tttggacttg gcgtgcaatg aagcggaacg ggctgacagg gaatttgata ttcttcaaga
    29641 ctatccagcc tattatgatg atgaaaacgt ctatgaacag agagataaat ggctggcaga
    29701 cttcatcagt caagtaccgc cagcaaagca ggacgatatt cgccagctca ttaaagaaaa
    29761 tacagagatt atctgataca cagggttatc gcaatcacgg catttatgct gggtgtgata
    29821 gcctttttta gttactgcca aaaaacagga acttggacgg aattaggacg gatttatctt
    29881 ttatgcttat cctgttgaaa tttgccttat tttggcaagg cggtttcttc acgggaggat
    29941 tacgaatgac aggagcgcat aaaagaaaat catcaaaaaa aatttaatcc atgttaaggg
    30001 agtttacagc tatttccgtg tagaatttcc tcaatatttg tcgattatat tcgctaaagc
    30061 tcaacgaggt tatcaaaact tcttgggctt ttttgctttt tctggatttc tttttcaaac
    30121 tttgccagtt catatcctct catttgtatt aatgacggaa agggggaagg aacggttaca
    30181 atcaccgcct tttgacaagc gaaaggaggt gaaaatgtga aaagacccac ttctccggaa
    30241 gatgtccaga aagtctttga ctgttactgc aagaagattt tgaaaaatga agcaatcaat
    30301 attcagaagc actaccagcg tatgaacgat ttgcagattt ccttttcaga actcaagcca
    30361 gaacagcttg cggaactctc gacttatgat gattattcca cggactatga cctttacaaa
    30421 gtcatgggct acgacgtgaa aatcaagaat gagctgttga gtgaagcatt gcaggaactt
    30481 tctgaggaag aacgctttat cttgttgctc tacggtttag gcttcaacga cggcgagatt
    30541 gccgacctgc tgaaaattgt gcgccgtaca gtgaactata agcgtcaaag aggatttgaa
    30601 aaagtcagaa aaagaatgga ggaaaaacag catgaagaag aataaagcgg tacataaagg
    30661 caatggctta ttgccttacc ctgttatcgt ttccgcttca cagggcgata ttctggctat
    30721 gaatgttgtg ttatgccatt acgagccata cattgcccgc ttgtccatgc gaacaagcat
    30781 tgacgaatac ggcaatcctc acaccagcgt tgacgaggat atgcgtagcc gtttggaagc
    30841 aaaactgatt gagcaggtac ttaatttcaa agtagcataa accaacgtcc tattgcagag
    30901 tgacgcatta tcccctttcc tgcgtcctct ctggggcttg tatatctatc aaaacccata
    30961 tgacttcata taggttttga caggcgtata agccgttgtg gtctttgaca actgaataaa
    31021 gcagacagat acgtttgtga tacagcgagc cacggggact gtacgccatg acctttccaa
    31081 aagagagcga gcgacccacg cagtgatccg agagcgacct ttggaaaagt cgccccgcca
    31141 tgacctgtat tcacaggata atgatacgtc ccagaggtgg ttttgctcat aggaatgaga
    31201 ttggttgaga taccagcgga gcttttccag agccgtctgt ctgcttccaa aagacacaca
    31261 gtaaaggggg tgcttataca tgacaaacaa tactgatgtg cctatctggg aaaagtacac
    31321 gctgacgatt gaggaagcgt caaaatactt ccgtatcggg gaaaagaaat tgcgtaagct
    31381 ggcagaggaa aatctcgacg ctggctgggt gattgtcaat ggaaaccgtg tgcagattaa
    31441 gcgaaaacag tttgaaaaaa tcattgatac attagacgaa atctaatgtc agcgagccgt
    31501 agatatggta taataaagta taacgtatca acggctcatt ccttgacgga aaggagcttc
    31561 gcactatgtc taatgtaaag agaaaagaca ataagggacg caatttacga cttggagaga
    31621 gccagagaaa agacggaaga tacgtttaca aatataccga tatatacgga aaaccacagt
    31681 ttaagtatgc ttggaaactt gttccgacag acaagacacc cgctgggaaa cgtgaagatt
    31741 tgtcgttgag agaaaaagaa aagcagatta agaaagacct tgccgacggt atcaatacca
    31801 taggcgggaa aatgacagtc tgccagctct acgcaaagaa gaacagccag aggaaaaatg
    31861 tgaaacgcaa cacagaaaaa ggtcgccagt accttatgaa aattctggaa aacgaccctc
    31921 tgggtgcaag gagcattgat acggtgaagc tatcggacgg taaggaatgg gctatgcgaa
    31981 tgtatgagaa tggcttttcc tacaagacga ttagcaatta caagcgttca ctgaaagcgt
    32041 cattctatat ggcgattgag gacgactgta tcagaaaaaa tcctttcaat ttcaaattga
    32101 gtgacgttct ggaagataat tcagagccta aagtcattct cacgccagag caggaagaaa
    32161 aaatgcttgc ctttatggaa caggacaaga cgtacagcaa gtattatgat gaagtggtta
    32221 ttcttctgga aacagggctt cgtatttctg aattttgcgg tctgacagtc catattgata
    32281 tggcgaaccg tatcatcaat gtagaccacc agctattgaa agacagcgaa atcggctact
    32341 acattgaaac gcctaagacc aaaaaaggag aacgccagct tccaatgaca gaaagagctt
    32401 atcaagcctt taagcgagtg ttacagaaca gaggaaaggc ggagcctatc atcataggcg
    32461 gttacagcaa tttcctgttc ctaaaccaaa agggcttccc taaagtggct ggtaactacg
    32521 aaagtatgtt gcgtgggctt gtgaagaaat acaacaagac acataaagac cagctaccca
    32581 acatcacacc acactcattc agacatacct actgcacgaa catggcaaac aaaggaatga
    32641 acccgaacac cctgcaatac gtcatgggac acgcaaacat caccatgaca ttagggtact
    32701 acgcacacgg tactttcaat tcagcaaaag cagaacttga aagactggct cttgcttctt
    32761 aaatcggtga ttgtactact catttactac ttttggttgc attttcatgc tggtaaatgc
    32821 cagcttatgc agagtatctt ccaaacggaa aatgccgata aagccctaaa atacaggcta
    32881 taccgacatt taccagctta tgaaaagata atcagaaatt tagtaagttt ttcaagaaaa
    32941 aagttatgat actgtaaaaa tattatgtag aaataatttt gattattttt taggtatttc
    33001 tgttgctgta actagaaaca actttatatt ttgttgcata ccaattatat atgctatagc
    33061 atactcaagt ttaataagaa cattgtcaat tgtaacgatt aaatcaacta tatttatatt
    33121 gatatttgaa tggtttttac cacttactat tttatctata gtgatatcat ttatttcagt
    33181 tattataaat aatttaatac taaagattac cttatcatca gtatttttat atttaacatc
    33241 aactaatatg ttggatagag ttattgatat taaagaacca ttttttaaag cattatttaa
    33301 atcacttaac atatttgaag atcaatcata tgcttactat tctgattttg tagggaaagt
    33361 taataattca gcatatgtta tagataagat tatacctaca ctatttgcaa ttactttaat
    33421 aatagtagca tatctgatat taaatgatat tagaaagaaa cagattaaat cattaatagt
    33481 tatagtagtg tacagtattt tatttattgg attaaattat atatcaatag atatgtcaac
    33541 atatagagat gaggagcgta gttatggaga gtatttaaag aaatctaata ttagtgatat
    33601 taaaataaaa tcgcatgata tgaagattga ctttaaaagc aagtcaaata taaaagatag
    33661 aattgtttta ttaaacacat caaatgaaga gttaacaaat attaatatga ttttagatga
    33721 aatatttgat ataaaatata taaaagtaaa ttcggaaaat ataaaattta acataaaaaa
    33781 ggatttaatt gaagttaatt taaaggaaag tttagagcct aatgacgagg tgacattaga
    33841 tatagagtat gaaggctata ttaatgtgtt gacatcttgg aatcaaaata aatatatagc
    33901 tactaaagat gatattatgc ttccaccagg ttctttagcg tggtacccaa aagtaaatca
    33961 atcaagttat atggcttata aaataaattt aagttctaaa agtaatgtct attcaaattt
    34021 acctttactg aataagacta gtaatttttt aaaaaaagat tatatatttg aaggaaagtc
    34081 aaatgatatt gcaatatatg caggtgagtt taaagaagag gtaaaagatg atataaaaat
    34141 aatctatcca tcagatagta atgtaaatat ggatatagag gaaaaaatta aatttttaat
    34201 tgaaacaaga aatgaaatat taggtgatag caaagcatct gtagatgcta aaaaatatgt
    34261 agatgaagta gataatgtta tggaatcaat aaaaagaaaa aaaattaata tgatattagt
    34321 tgctgactta cctagcaatg aatttttata tgaagactca aagggagaga aaattagcat
    34381 aactatttat cattggcttt caggaaatat ccttataata gataaataat aaaggaggtt
    34441 aatatgataa acaagttaga aataagaaat ctaaatttta attacaaaaa taaaaaagct
    34501 ttggataata taaatttaac cctaacagat ggagtagttg cattattagg tcctaatggt
    34561 gctggaaaat caacattaat gaggttatta gtgactcttt atgaaacttc aattggagag
    34621 atagaattaa ataatataaa gtatagcaaa aacaatgaga aaatcaaggc aaatgtagga
    34681 tatgtacctc aagattttga tatgtataat aatataaatg ggcaagaata cttagaattt
    34741 gtagctaaaa tgagaggtgt atctaagaat gatttaaaga aacatataca aaaagtagta
    34801 tcaaaagtta atctggacaa gtttataaat aaaaaaatag gtacatactc aggtggtgta
    34861 aagagacgac taggtatagc acaggcttta ataggtgatt ctaagttaat agttatggac
    34921 gaacctacag ttggtcttga tccagagcaa aggaatgaat ttagaagatt gttaccagaa
    34981 ataagtaaaa attctatagt attaatttca actcacatag tagaagacat acaatttaac
    35041 tgtgacaaat taattatatt aaatcaaggc aaaatattat atgatggtac aattaataag
    35101 tttattgata tggttgaggt gtattcggta gtagtttcta atgatgaatt tgaatattta
    35161 gaacgtaata ttgatattat tgattatagg agagttaaag atggtgtgca agtaaagtat
    35221 ataggtaaaa atacagagaa acttaaagat agcttaagga tagatacaac tttacaagat
    35281 gcttatataa actttttaca taaggagtta aaataaatgt gtatattgga gttaataaaa
    35341 tttgagctta aaaaaataga gcttaaacat gtgttaatat ttgtaattat ttctatatta
    35401 tgcatattta ttacaggcat attagattat aatgagttaa tattttataa tgatgtacct
    35461 ataatggata catattctat agcaggatgg ttaggaatta taaagatatc agatgttttt
    35521 ataccaataa tgtcagcaat cacaataatt tatgtgtttt caaaagatta ttcgggtaat
    35581 ataaatgaga tacttacttt atataataaa aaaaaatata atcaatttat agtatttaga
    35641 tggtgtgtta tattagctat atatactgta t
//
LOCUS       DAKBNN010000014        35441 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000014 DAKBNN010000000
VERSION     DAKBNN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.014"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(11..2566)
                     /locus_tag="OUB02_000726"
     CDS             complement(11..2566)
                     /locus_tag="OUB02_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8884520.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(2563..3249)
                     /locus_tag="OUB02_000727"
     CDS             complement(2563..3249)
                     /locus_tag="OUB02_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884521.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     IVVNTGGEVL"
     gene            complement(3326..4234)
                     /locus_tag="OUB02_000728"
     CDS             complement(3326..4234)
                     /locus_tag="OUB02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8884522.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(4236..4907)
                     /locus_tag="OUB02_000729"
     CDS             complement(4236..4907)
                     /locus_tag="OUB02_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884523.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            5169..6233
                     /locus_tag="OUB02_000730"
     CDS             5169..6233
                     /locus_tag="OUB02_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HCT8884524.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            6391..7077
                     /locus_tag="OUB02_000731"
     CDS             6391..7077
                     /locus_tag="OUB02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884525.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTVKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            7067..8098
                     /locus_tag="OUB02_000732"
     CDS             7067..8098
                     /locus_tag="OUB02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8884526.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALTIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIHVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            8180..8845
                     /locus_tag="OUB02_000733"
     CDS             8180..8845
                     /locus_tag="OUB02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884527.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            8858..11275
                     /locus_tag="OUB02_000734"
     CDS             8858..11275
                     /locus_tag="OUB02_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8884528.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(11456..12247)
                     /locus_tag="OUB02_000735"
     CDS             complement(11456..12247)
                     /locus_tag="OUB02_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8884529.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(12280..12867)
                     /locus_tag="OUB02_000736"
     CDS             complement(12280..12867)
                     /locus_tag="OUB02_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8884530.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIYNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(13044..14435)
                     /locus_tag="OUB02_000737"
     CDS             complement(13044..14435)
                     /locus_tag="OUB02_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HCT8884531.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(14625..15923)
                     /locus_tag="OUB02_000738"
     CDS             complement(14625..15923)
                     /locus_tag="OUB02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HCT8884532.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIQNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(16282..17157)
                     /locus_tag="OUB02_000739"
     CDS             complement(16282..17157)
                     /locus_tag="OUB02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCT8884533.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(17350..18036)
                     /locus_tag="OUB02_000740"
     CDS             complement(17350..18036)
                     /locus_tag="OUB02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCT8884534.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(18085..18618)
                     /locus_tag="OUB02_000741"
     CDS             complement(18085..18618)
                     /locus_tag="OUB02_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCT8884535.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(18662..19294)
                     /locus_tag="OUB02_000742"
     CDS             complement(18662..19294)
                     /locus_tag="OUB02_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCT8884536.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLETVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(19384..20463)
                     /locus_tag="OUB02_000743"
     CDS             complement(19384..20463)
                     /locus_tag="OUB02_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HCT8884537.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(20596..21942)
                     /gene="pepV"
                     /locus_tag="OUB02_000744"
     CDS             complement(20596..21942)
                     /gene="pepV"
                     /locus_tag="OUB02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCT8884538.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTIAAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(22011..23291)
                     /locus_tag="OUB02_000745"
     CDS             complement(22011..23291)
                     /locus_tag="OUB02_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HCT8884539.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(23418..23678)
                     /gene="hfq"
                     /locus_tag="OUB02_000746"
     CDS             complement(23418..23678)
                     /gene="hfq"
                     /locus_tag="OUB02_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCT8884540.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(23792..24727)
                     /gene="miaA"
                     /locus_tag="OUB02_000747"
     CDS             complement(23792..24727)
                     /gene="miaA"
                     /locus_tag="OUB02_000747"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCT8884541.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(24765..26732)
                     /gene="mutL"
                     /locus_tag="OUB02_000748"
     CDS             complement(24765..26732)
                     /gene="mutL"
                     /locus_tag="OUB02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCT8884542.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKKSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYSCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(26743..29586)
                     /gene="mutS"
                     /locus_tag="OUB02_000749"
     CDS             complement(26743..29586)
                     /gene="mutS"
                     /locus_tag="OUB02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCT8884543.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(29747..30481)
                     /locus_tag="OUB02_000750"
     CDS             complement(29747..30481)
                     /locus_tag="OUB02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884544.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIKVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(30450..31463)
                     /locus_tag="OUB02_000751"
     CDS             complement(30450..31463)
                     /locus_tag="OUB02_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8884545.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(31485..32255)
                     /locus_tag="OUB02_000752"
     CDS             complement(31485..32255)
                     /locus_tag="OUB02_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8884546.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(32230..32520)
                     /locus_tag="OUB02_000753"
     CDS             complement(32230..32520)
                     /locus_tag="OUB02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCT8884547.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(32651..32750)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      33370..33459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            33512..33688
                     /locus_tag="OUB02_000754"
     CDS             33512..33688
                     /locus_tag="OUB02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884548.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(33803..34159)
                     /locus_tag="OUB02_000755"
                     /pseudo
     CDS             complement(33803..34159)
                     /locus_tag="OUB02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            34415..35329
                     /locus_tag="OUB02_000756"
     CDS             34415..35329
                     /locus_tag="OUB02_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCT8884549.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNNLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVPPPLQQTYQKRLLNAFGILKKDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGHYIFTLHNKETLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKMLV
                     KVIILDNKPVEVKIIGKAVIAFSTNIEI"
BASE COUNT        11961 a         5647 c         4008 g        13825 t
ORIGIN      
        1 ttatataact ctaattctta tttatttctg aaataatatc actcttcaaa atttgtctta
       61 ctggaataat tacagcaact atactaatag tgatatttaa aataatcata cctatgtata
      121 gaggtgtatt tattttaaga attggattta tatataagta tacacgtttt accataaaat
      181 aaatcataaa ttgtccaata acacttccaa ttaccattat aatacttgca tagaatccat
      241 ataaaaaacc ttctcttatc atcataagta aaaacttatt atccgtaata cccatagctc
      301 ttaaaattcc aaactcatgt cttcttgaaa aaattaagta acttactgta ttgattatat
      361 ggaacaaact tattgcaaga agtacaaata caataccata tataaataag agtttttgat
      421 ttaagtaggt attatttttt tcaatagcaa tggtataatc tgttacaata catcttttta
      481 catcacttac aacacttttt atgttctcag acaattcctc actgttacta tcttctgtct
      541 tttttatact tactgaatta taattttcga tatcaaagtt atttttcatt tgcccattcg
      601 tcataactat atccatacca taatcaccaa tgaaatatat gttatttgcc atcacacgtt
      661 ttacagtagc agcaactaca aattcttttg ttgtataatc attcttattt tcttctttaa
      721 atttaacaat ttcatctgtt ggtttcattg tctttggcac tttaactttt attgtatctc
      781 ctggatttat atcaactgca tcatacatcc cagttccatc cataggtagt ctaactataa
      841 ccttattatt tttaaccata tcatgggtat taatctcccc atctataata taagaattta
      901 atccttctaa cataccattg ttataaccat aaatatttgt ttttaaaaga tagccacctt
      961 cattttgctt tgtacaaata ccatcaaaaa aagtctttat cctgtaatct ttttcaagag
     1021 gtttaaagaa atttttccaa aatagttgtt cttctttaat ttgcatagct ccaaataaat
     1081 atcttactgg atagactttt gatattccct caacattgtt taatcttgaa attttatctt
     1141 ttggtatacc tacatcaaaa tctgttgttt gcatagttat ttggtagtca gaatttaaac
     1201 catcatcagc tttcattgta agttcattgt tatttttagt aagctgtata gagtaactag
     1261 aacatagaaa tataattcca ccaagtgata atgaaaacaa aattccaata aacattccct
     1321 ttttttgagt catgtattta tgagataata cttttgtcat gttagagtgt tttacacttt
     1381 ctaatattct ctttttctta ttagtaatct taaagttttt catagattct atactagagt
     1441 atttattcaa ctgatgtaca acacgtacag caattaaaat taacaacaat aagataaata
     1501 aaaagcctaa ataaatagat ttttcactta taaaaaatgc tcctggaaca atatcactac
     1561 tcaaaaatat atgtggaaat tgttcatata ttgtagacgc tactccaata cctaagaagc
     1621 aacctatagg aaatccaaca ataaaaagta aaaataactc taaaaatagt agaaataaaa
     1681 gttgtttatt attagcgcct aatacttcta aaaccccata ttgtgatatt ctctgaagta
     1741 ttgaaacatg gaaaatacta tatactgctg ctgcaccaaa cactccaaca agcaatataa
     1801 ttgcatttgt atttagttct aaatttttta agaactcaaa tatattatat ttttctatat
     1861 tttttggtgg ttttactcca aattcaactg aaagaggcca gtttatataa gttttttctt
     1921 ttgatatgtt gaattttttc ataaatttat aattttgctt tttaatattt ttattttcat
     1981 catattttac ataaactata taagaatcct tttctttagg ctgaaaataa tcgtttacaa
     2041 atccctccag agcagtttct gggtttgaaa gacttgataa aataccacat aacttataga
     2101 ttttaccatc taaattgatt tctgttccaa tttctttgtt tacatttaaa ttgtttagag
     2161 aatatgtgtc aagtactatc tctccatctt tactaggaag agttcctttt aataattttc
     2221 tacctgtcat atctaaatat gaagaatcag catttaaaaa ttctataata aaaggttcat
     2281 ctatctgatt tttaggtttt gtaacgccaa gtctatctac agtgtattcc tttgattttt
     2341 tattttcttt aattttatta agttgttcct tatctacttt atagaaataa tgataatctc
     2401 cactatcttc ccttatcttt tcaattctac ttttgtctgc actatgaact aaagaaccaa
     2461 ttcccgtcaa taatgctact gatagtacta tgcttaaaac tatagatata gattgttttt
     2521 tataatgctt tatatattga attgaaaatt ttactagaga catcatagta cttcaccacc
     2581 agtattcacc acaattccat cttcaatatg gataattgag tcgcatactt gtgctatatt
     2641 ttcattgtgt gttaccataa gtatcgtttg atggaatttt gcactacttt cttttaataa
     2701 acataccact tccattgtag ttttactatc gagatttcct gttggttcat ctgctaatat
     2761 aagcgcaggt ttatttgcca aagctctagc aattgctacc ctctgttgtt gaccaccaga
     2821 tagttcgctt ggaaatttta aacgcttatc ccaaattccc aatgtcttca tcagcatttc
     2881 aatatattca tggtctatat ggtttccttt atctaatgta acaggaagag ctatattatc
     2941 ataagtgttt aatacgggca ttaaactgta gttttgaaat ataaatccta tatttcttct
     3001 tctaaacact gtcaaatctt tttttcctat tttagatatg ttctttcctt taattattac
     3061 atcacctgaa gttggattat ctaatccacc tataatattt aacaatgtgc tttttccaga
     3121 accagaagtt ccaactatag cagtaaatgt atctcgttca atttctatac ttactccatt
     3181 taaggctttt actttagtct caccttttcc atagtgtttt actaaattat tcgtcactaa
     3241 tatactcatt taatttctcc tttcaataca gatatatgtt ctataaaata ttgtaagtga
     3301 cgataattac actcatccta caaaattagt ttaaactctt acaattctgt aagaagatac
     3361 taaaacaaca tccacttcct agtttagatt ctactataat gtttccattt tctttttcta
     3421 aaattaatct tgtaagatat agaccaatcc ctgtgccatt ttggttttcc acatttttac
     3481 ttctgtaaaa tcttttaaaa atatcattta gttctctact atcaatacct attccctcat
     3541 ctttaatagt aatcttcgta tatagttcca tttttattac agaaatagta atcgtgctat
     3601 ctgatggtga gtattttatt gcattttcta aaacatttac aattgcttct atggtccact
     3661 ttttattatg aactaatttt atgtctgaaa atggttcgag atttatcctt atatttttag
     3721 cttctgcttt attaaagata gtactgatac tttgaataag agtatcttta attagtagtt
     3781 cttctacttc aaattctata actccatcct ctaatttagt cattttaaat aaggattgta
     3841 acagccagtc aatcttaaaa gtttcatttt tcatcttttg taaaaactcc ttcttctgta
     3901 atgtggaaat atttatattc tccagaagtt cacaataaat ggttatattt gaaagtggag
     3961 tttttaattg atgtgacata tttgatatta aacctttaat cgcttctttt tctgactttg
     4021 actgttctac ctctatttct atcatatcct taattttttt tacttggtga gaaatttttg
     4081 acgctcttgt atcctttgta tctggataag taacttcttt accttccaat atttgattta
     4141 acatcaaatt taattcatca tatcttcttt gatttatttt agtagatact ataaatataa
     4201 caactgtaca tattataaag gctaatataa acatattatt ctacctcatt ccataaataa
     4261 cccattccat aagctgtttt tatatatttt ggattagatg ggtcatcctc tacttttact
     4321 ctaagtcttc ttatatttac agatacaata ttatcatcaa cataattttc ttcgctgtcc
     4381 caaatatggt gtaaaatttg ttcctttaat aatatttgac ctttattttc aattagataa
     4441 cttagaagct tatattctac agagctaagt tccaaacatt ctttattttt atatactttt
     4501 agtaactttt ggtccaattt tattccattg gaatatatta ctttattatt cgattttttt
     4561 cttattgcta ctttgactct tgcctttaat acagataaag aaaatggttt tgtaatataa
     4621 tcatcaagcc ctatttctaa tccttttact tcatcaattt ctgagtctct tgcagtaagc
     4681 attataattg gtatatctga cttttccctt attatttgac atacatcaaa tccatctcca
     4741 tctggaagat tgcagtctaa gattataaaa tctatatttt tactattaaa tatatttaat
     4801 ccttcttgtt ttgtataagc cttaaataca ttaaatccat cattctcaaa tgcataaaaa
     4861 agaccttcat ttaagtttat atcatcttca ataattaata agttcaattt tactctcctt
     4921 tagatttttt agataaataa ttattttctt gcattatcat actaaaaata ttttaatttt
     4981 atttatttca ctaaaaagat tgtatttaaa tttgttttca aaataaaaac atcataatca
     5041 agcatatagt tatatatttc ccaatttaat tacgcttaaa ttgaaaatat aactttattc
     5101 ttatataatt aaattataca acaataaagt tatatttttt aaatatattt ttactagggg
     5161 gaatagaatt gaaaagggtt gatgagagaa atactatgtt tgcaagagca aattacaaaa
     5221 aagatagtac tgcttataat gactattata agaaaaatcc tgacaaaaaa gaaattgatg
     5281 atagtataag aaatagacca aatttatgta gtgaaggaac aatgacttac aatgagttaa
     5341 actctccaat ggcaagtagt gcattcgatt ttttatctga tatcaaatct ttatgcgaag
     5401 gaaaagtttc tgatactaaa gtagatgtag atgcaaagtc aatgacaaaa aaaattaagg
     5461 gtcttgcaaa gcagtatgga gcttctgtag ttggtataac taaattaaaa gactatcata
     5521 tttatacaaa taggggtaga catgaagaaa attacggtga agaaattaat ctaactcata
     5581 agtatgcaat tgtatttgga tgtgagatgg ataaagaaat gataaataga gctccaatga
     5641 tatgtgaagt tatagaaaca tcaaagtgct atgtggatgc atctatcgtc ggcatgattt
     5701 taagttacta tataagaaat ttaggatatg atgctagaaa tcatatggat gctaattatt
     5761 tagtaatgcc tgtttttata gctcgtgatg ctggtttagg agatatagga agaaatgcta
     5821 tacttacgaa taaagattat ggctctagac ttagattagg tgttgttact acagatattc
     5881 ctcttttaga agatgaatat gttgattttg gactagaaga tttttgtaag gtctgtaaaa
     5941 aatgctcttt taactgccct tctcattcgc tttctaatga tattaaaatt ggtgatgatg
     6001 gaaaatataa ttgggtaatt gaacatgaga catgctatat taaatggaga tatttaggta
     6061 cagattgtgg tatgtgtata tctgcttgcc catttagcca aaatctcgaa actataaaga
     6121 atactacttc atttaagggt aataatgagc ttattcaaaa agctttagat gagtatactt
     6181 caaaatttgg taaaagaata tttatacctg gaaatccatc ttggttgaaa taattttatt
     6241 taataaagaa tctgtactag aatttatcat aggacaggtt ctttattaaa tatataaatt
     6301 tttaaatact ttttctcgtt tataacaatt cagcaatgtt tttagtctat aattgacatt
     6361 ataaaatatt aaactaaaag gagtaactgt atggaaaaaa tattagtagt agaagatgat
     6421 tctatattaa ataaaactct atcatataac ctaattgaag atggatatat aattacttca
     6481 aaatttacag ctaaatctgc actgaagtct atttttgaat gtgaatttga tttaataatt
     6541 ttggatatta atttaccaga taaaagtggg tttgaattat gtaatgaaat aaaggaaaat
     6601 tacaatatac caataatttt tttaactgct aatgatatgg aatgtgatat gattaaaggc
     6661 tatgaacttg gagctttgga ttatataaca aaaccattta atatcaatat atttaaacag
     6721 aaagttaaag cttttttaaa tcatcttaca gtaaaaacaa aacaagatta ctatagggat
     6781 ggatatcttg aaattaactt ttctgagtta tctgccaata taaatggaaa tcaaattata
     6841 tttacaccat tagaatatcg tactttaaaa ctgcttactg aaaatcctaa aagtatttta
     6901 acaaggaaag tacttttaga aaaactctgg gatatagatg ctaactttgt agatgaacat
     6961 actttaacat ctgttataag taggattaga agcaaaattg aaaaagacaa tcttaagtat
     7021 atcaaaacgg tatatggtat gggatatatg tggttaggtg aaagaaatga attttaaaaa
     7081 attttctttt agttctattt atttaataat tgctgttggt ataattttta cagctatttt
     7141 atttagtatc tcaatttatc taataactga tgatttaaaa ctaattgctc tattattaat
     7201 atatacaata gtactttgtg gatttatagc attaacaata tttttacttc ataaaaaaat
     7261 agttatattt tgttctaaaa tatgtcacac tttagatagt atgatgaata gtgaagacaa
     7321 tattgaaatt gaattagaag aagaaactat tttatcacgt atcaatcaca gattaattag
     7381 aatgtatgaa gttattcaag aaaatcgcaa tagtattgct atagagaaag ctgatttaca
     7441 agaactagtt tctgatatat ctcatcaaat taaaactcct attgcaaatt taaaaatgat
     7501 taatactact ctactaaatc aaacatttaa aacagaaatt caaaaggatt ttttaatgga
     7561 tatggaaaac caattagata aattagattt tttaatgcaa tctatggtaa aaacctccag
     7621 actagagaca ggtataatca cactatctaa aaagaaaaat tcaatttatg aaacaattgc
     7681 tacagcactt agtggaattt tgtttgatgc tgaaaaaaag aatattgaag ttacagttga
     7741 ttgtgaccca aatttatata taattcatga caagaaatgg acttctgaag ctatttttaa
     7801 tatattagat aatgctgtaa aatacacaaa ttctaatggt aagatacatg tagctacaga
     7861 atgttgggtt atgtatacaa aaatagatat tattgataat ggaaaaggaa tatcagaaag
     7921 tcatcaagca gaaatcttta aaagatttta tcgtgaagaa gatgtccatg acatagaagg
     7981 tattggaata ggtctctatc tttcaagaaa aattatatct cttcaaggtg gctatattaa
     8041 agttacatca gatattggaa aagggtcgac tttttctatt ttcttaccta ataaataaat
     8101 ttttaaatat ttcatttgta atgattctgc aacattttgg ttttatactg tatttacaag
     8161 atgttaagga ggtattatta tgagtatttt acaagtaatt aatttaaaga aatattatgg
     8221 acaaagttca aacattacaa aagctcttga tggtgtaact ctatctattg aaaaaggcga
     8281 atttatttct attgtaggta catctggtag tggcaaatcc acattattaa atatgatggg
     8341 aggattagat aatcctacat ctggaaaggt tattataaag ggtaaggaat tatctactat
     8401 gactgatgaa cagcttacaa tctttagacg cagaaatata ggttttatat ttcaaaatta
     8461 taatcttgtt cctttactta atgtatatga aaatattatc ttaccaatag gattggatgg
     8521 agagaccata gataaggatt ttataaatga aattataaat atgctttctc ttgaagaaaa
     8581 attatatgat atgccaagta atctttctgg tggtcaacag caacgtgttg ctatagctag
     8641 agcacttgta acaaaaccag ctattatact agctgatgaa ccaacaggta accttgatag
     8701 tagaactagt tcagatgtac tgagtttact taaagtgaca agtaaaaaat tcaaccaaac
     8761 aattgtaatg attacacaca ataatgaaat tgcacaacta actgatagaa ttattcgtat
     8821 tgaagatgga aaaattgtta actaggaggt gtagtatatg tttagaaata ataatcgcac
     8881 atttataaga aaaatagctt taaatgattt aaaaattaat aaacttaaaa catatctttc
     8941 aggtatcatt ataatgattt ctacatgttt attattaaca gtgaccttag tttcatataa
     9001 tgcctctgta gatttagtta atgcatctcc atatcatgct atatataaat ctgtagatga
     9061 aaaagcaaaa aatatactat ataaagataa aaactttagt ggcgtaggaa cttacaaatt
     9121 aattggaagt aataaaaaga cagattgtat tatgtcgatt gtttatgcag atgatacagc
     9181 aattaaactt atgaattttc aaccattgaa aggcaaacta cctacaaata aaaatgaaat
     9241 tgctatttca gaaaaatatt tacaggaatt tggtttagat aaagatattg gagatagcat
     9301 taaattaaat tattatgatg atattacgaa taaagaagtt caatgtgatt ttattattgt
     9361 tggatttcta gaaaattatt atcaagataa tgccaagcaa tactatacag tcgtttcaaa
     9421 tgattatttt aagaatatag cttctacacc tttacaaaat aaaagtaata cttttgacga
     9481 gtcaagacct gatactgtag atgtattagt taaactatca gaagaaaata cagagaaaga
     9541 ttcagcttca attaagacac agttaaagaa aattggttta tctttaggta ttaagaaata
     9601 taatatatac ttaaatgata actatattga atcaaattta attgatggtg agcagattat
     9661 tacagtttta gtagttggaa tagttgttct tttttctagc gtttttgtta tatatagtat
     9721 tttttatatt tcagttgtta attcagttca gatgtatgct aagttgaaat cgttaggtat
     9781 gacttctttt cagttaaaaa agattatttc tctacaagga aatattttgt caattatttt
     9841 tattccatta ggggttatag cttcttgtat aattgcttac ataattcaac cattagcatg
     9901 gcaaatgata gctgatttat ttataatttt aattttatca ctggtaatgt ttttaactgt
     9961 tagaatttct ttatttaaac cagctagaat tatttctaaa atatctgcta ttgaagccat
    10021 gcaatataca gaaactaaat ttagaaaaaa agcaaaaaat tttagttata taaatataaa
    10081 aaatctagca ttaaagaata tcgagtcaaa tagaaaaaaa aatttgattg cattaatatc
    10141 gctaagcatt agtggagttt tatttatttc aatcgctaat cttgctaatt caatagattt
    10201 caaaaaacaa ctatctcaac aatttgtcta taacgaagat tatataatta gcattaagag
    10261 tgataactta tatgagcata ttacagaaat ccaacaaaat aatccactaa cagattctct
    10321 taagaatgaa attaaatcta ttcatggagt aaaaaaatta attgaatcca aatcaattaa
    10381 aagtaatatt atagaacctt acatcaaaga tagagacgaa aaacaattta ttactctaat
    10441 taaaggtatt acgcctgaac tttcaaaatc acttgaagaa tatattgtca gtggaaaaat
    10501 agactataca catttagatt cagatagttt aattataaat aaatatagaa caaaatatta
    10561 tggattaaat ttaaaagttg gagatacagt tacatttaat atctttagtg gaaatgatat
    10621 tattaaaaag aaaatgaaaa ttataggaat cattgacaat tccaatacag gaggtatgtt
    10681 ttttgcttca gacaaaactt tagaatctct aacaccatat aatacaaatt tagatttttc
    10741 tattttaata gataaaaagc attctaaaga agttgaagaa aagttgttaa gtataattca
    10801 gaaaaattca aatttaatgc tttattcata tgaagatgat tatagaatga ttactagagc
    10861 atttcaatat ataattatag cttcatacat ttttgttggt ttaatttctt gctttggtat
    10921 actaaatatg accaataccc taataaacag cgttttaatt agaaaaaaag agtttgcatt
    10981 acttcaagcc gttggaatga caagaaagca actgcgaaat atgctttata gagaaggtct
    11041 aaacataagt atcaaagcta tatgtacttc aagtatacta ggttattttg gttcaaatct
    11101 actttgcact tttataaaag atgtaattag attagacttt attaatttta aattttctat
    11161 atttactatt ttaatttttt cattcgtatt aattggtata caagttttag tgactgaatt
    11221 acttgtaaga aatatagaaa aaaaatcact tacagaacgc ttacgttctc agtaagaatg
    11281 tatataaaaa atagccattt tttatacaag ttttaatatg attttttaaa agctataggg
    11341 atagaaaatt ttgactttac ataattttct atccctatac ttgtttttat tttatacaga
    11401 tacacttact tatatctccg agcaacatca ccagagacat aaacaagcat attccttatt
    11461 ttctaaatat gtcaattgca tgacttgtaa cccctactaa atcttcacgt tcacaagtag
    11521 caaggtgata ttttaaatat aatgcaaatg catcatcatc catagagtct actgcttcac
    11581 gcatatacaa agccaaacca tctgatgcga cataatgcag tctagttaca ttaaatgcag
    11641 acatcaaatc atcaatgttt tctttacgta caagctcaaa caaatccttt ggttcagact
    11701 tagcagcaaa tgtctgtgga tcaatcaaac ctttttcaat atattcagat acattaatat
    11761 ttcctctatg gaacccctcg tcaatgagac agccatcaga tataacatat gcagcaaaaa
    11821 ccacaccacc aggttttgtt acacgaattg cctcatgtaa agcttgttgt ttatcttctt
    11881 tgttataaag atgatatagt ggtcctaaca gtaacgtaat atcatattta ttatctggga
    11941 aaacagataa atccatagca ttaccctgag taatgcttat attttcattt gattgagtat
    12001 ttttatggaa aacctctata ttatgttcga ctaattccac tgcgtcaaca tcatatccct
    12061 gacgtgccag tgtgtgagaa taacgaccag ttgcagcgcc aatttcaagc acacaatcac
    12121 ctgattttat gtacttatca atgtaatgca tagtagtgag aaattccacc attccatgtt
    12181 ttaatgcaag tcgactatct tcatcataag tattataaaa gtcaattaag tattgttttg
    12241 tttccataat ttcacctctt ctatattagt taataaacat caaatagaca aattccaatc
    12301 tggtttttct tgtcttataa aagctgttaa tatatttttc aacatttttt cagcactata
    12361 tctatctaat gttaattttt ttgttgctgt taagacagct atgccatgag tataaagaaa
    12421 taaatctaaa aatattactt ttgcacattc caaatctatt ccaatgcttt ccgaaaaaag
    12481 tcttgccttt ttttcattgt aaatttcttt ataaaaatca ttcgcctctt tcatcatcaa
    12541 atctaaatca tttataaata acaacttaaa taaatacgtt tcttcttgag caaattcaat
    12601 gtatgaaagc ggaagcagta aataagggct tacactttta gaattacgat aattaacaac
    12661 atactgatta taatattcat atgcaaaagc aagaaattca ttttttaatt cattcatgtt
    12721 ttcgtaacaa gtaaaaattg gtcgagtgga acactgtaat ttacttgcaa tactcctcgc
    12781 atttacagct tcaaatccca cttctcttgt aatatccaag actgtattca aaatcatttc
    12841 ttttgttatc tttactttag gtggcatata tgttcacctc tccattaaat ttttataaaa
    12901 cacatatgtt atataactat agtaacatat caagaaatta tgtcaagaaa aagctttgca
    12961 tttttttgca attcctaaaa cttataaata aaacatgagt tgcaattaat ctattaatag
    13021 ataacagcaa ctcagatttt cctttaaata ctcttctttt tgtctaatat ttcatataca
    13081 caatcttcta ttgaattaat atctttttct tttctttttt tagaatcaat taaaatgaaa
    13141 tacagtataa aacctacagc aagtccccat gttccgcctt tagcagcaat tacacctccc
    13201 ataagtccaa tcaatccctt ctcaacacct gtattacata gctccattga tagctttgta
    13261 cataaatatc cttgaactaa aagtgttact gacaatgcta atggtataag aggtgtagca
    13321 atctgcgcaa ttggataaaa acagataaaa attgctgaaa cccatactaa tgtacccatg
    13381 ccactaaaaa cagactgcat ggcttccttt ccaaccttgt acctttgagc aacacttcct
    13441 gtaagtgtcg cagctaatgg accgcacatt ggtatatatg gagaaaagat tgccataaaa
    13501 atatttctta tcccacttat aacattagac ctatttgaat tgaaatctat atattcatct
    13561 tttctatttt cactttcaga aagtactaag ttttcacctg ttataaaatc accaaaggca
    13621 attatgtata tcataaaagc cattggtatc ccttgtataa ataacattac acttggaaaa
    13681 cctattccaa aaggagatac tgtatttatc agatttttaa aatctactat gtaaactaaa
    13741 ttgtcaaaac taaatctagg aatttctatt tctttactaa atatacctac tattaaactc
    13801 actaaaatag ctgatactac accatattct cctaaatgaa ataaaaactt attcttaact
    13861 tttagagtat caaatctttc agaaaacatt acaaaacaag caaccaaaat tcctactgtt
    13921 atagatatag gataagaatc aaatctccct ttaggattaa actctgatat tatagtcgat
    13981 ataccgacac ccattagtat tccaccttta atagaatttg gtatcttact aactaacttt
    14041 ccagctacac cagttatacc cattaaaata aatattattc ctaaatctat ttgtaaagct
    14101 gttaatgctt gtattctttc aatgcccatg ctgaacttta acaaatatgc agttgtaaga
    14161 gttatagaag ctgtaatcca gccaggtaca actgggtctc ccataaaaac atgcaaacag
    14221 tataatattg agttaatgat agccatactc catgccactt catagggtaa tcccaaaatt
    14281 tgtgtactaa ggccaattgt gcctaaactt gtcatacttg atattatacc ttgaatagct
    14341 tctggtttct caaatctgta atgtataaat ggaattctta tcttaaacat tccaaatttt
    14401 atatgaggtt gttcccttct agcgagtctt tccataactt cactccacct ttcaataact
    14461 cttataagtt tacaacttta taattgttta taaattatca atgtcattaa aattagtata
    14521 taacatgcaa attatataaa caataaatta aatctataaa attgatagat tttgtgcata
    14581 tatagaaaga ccgcttcatt tttagttact taaatcactt aatattagtt atttaaatca
    14641 cttgataaag gttttttata aaattcaata gttctattcc atgattcaaa ctgtcctttt
    14701 gaatttcctt ctacagttcc acctgaagaa aaaatccctc cactcatact catacataaa
    14761 gtctccttta gaggcataat atatggaatt ggtaacggat gacccatatt attgtatgtt
    14821 aataattcta cgttatatgg ataattattt tcttcaaaga tttctaatgc ctttaaacaa
    14881 gcttcataac tattccatat gttatcttct tttccagcaa tcataagtat atctgctttg
    14941 gaattttcta tttttattct agcctctttt ctattctctg ctcttttaag acttttctta
    15001 taagtactat taaatccaaa tggaatacct tttgatatat tttttttctg ttcttcaaag
    15061 aatatatcat tgtctacttt gatatatgga aatggctctc ctttatatga ccatgatgaa
    15121 gtatcatttc cactcatcaa tccattaaga gcttggaaac aataaatgtg aggttgtgat
    15181 actacaacct ttttaatttg ttcatatctt gaagcaagta aaaaggccaa ttctccaccc
    15241 tttgatgtcc catgtacaaa tatttcttta gtactggtaa tttcattttc actaatccac
    15301 tcaaatactt tttcaaaata ttctaatgga acctcttcta atttatctgg taacccttct
    15361 aaagcaaagt atgcaactgt tagtacattg aatcctctag atgcaagtgg agctgctaaa
    15421 aggtctagag cgtctaaatt tccatcagaa ccacctagca ttaaaattgt tttattatta
    15481 gaattttgtt tataaaataa ttttcctata aactcatcag atatgtattt tgtggtaata
    15541 tcttctgatt taaatattct ctttaattca acttcttttc tttcaataga agtttcaaat
    15601 atcatattca taattattgg tttatcaata gatatatttt ctgcaaaatt tttaccttca
    15661 gttttagatt tttccagcga ataaattaac cccatacaat tatcagcctt ataagtacct
    15721 tctataggtt caactaaatc taaatctact tctccttttt catttgaaca aaacactcca
    15781 taagatgaaa attcttctcc cttgcaccat ggaaattcca ttttcacact tattttaagt
    15841 ttagtatttt gaattaagtc cgaaaccata atcttaagtc tttcatccac aaatgattct
    15901 acattgtata catttacttt cataatctat ttctccttat aataattatt tatgttttat
    15961 ataaaacagc tttatctaat gcaacataaa taaattttta aattaagaca ccatattgac
    16021 tatttcattt tatcaacata ttccatattt gtcaacatat tgtctttttt agtaatttaa
    16081 aattatgtag tctatatgaa atccagttga tttttggcta attaaagata ggaatgctat
    16141 taggtttaga atatatatat tcaaaataaa aaaccactca tttaaagaat ggtttttaat
    16201 ataaactatt tgttattaat aaggatattt ttttatttaa atttatataa tcgaacatct
    16261 aatatttaat gtttaataaa attatgcatt tgcaaaagta tatatggcat taactcttgc
    16321 tacatataaa ttctcatcac tacatccaat atatctagca tatttttcaa tcttttcttt
    16381 tgcattttca acatcatttt catcgctctc cattaactca tttatataaa tatccattaa
    16441 tgacgcttta tattctctta tatcatagcc taaaaattca gtcaatctct caatttcctt
    16501 actaaaatct ccatcttctt ttaccaaaaa ttcgtataca tcttccctat atggatttaa
    16561 agttattatt tgttttacta ctttgcattc gtcagccttc ccaataaaac catctttata
    16621 attattaaat aaggcattag atttttttat agattcttca tcaatataag aatgtaccgc
    16681 agttatacga ttttctataa gggcatctac aacagcataa tgaattttaa aacaatcatt
    16741 gtataaataa ttgctcattt tctttattgt cgattctatt tctaagaagc tcgcgtggcc
    16801 atttttttta tttttatttg gtataataaa agttttattc aaattattaa aaagtctctt
    16861 aaaatctaaa tatttttcac aataaacact tttaaaggta tcgatatcta tagttataat
    16921 tccaaaactt actatagtct caattccttt cttaaaagac tcttctatat atttttctcc
    16981 taaacttaaa cctctatcag atatttgctt tagattttga aaactattta aacaatctct
    17041 ctcaaagttt ttagatattt ccaaagcata taatttaaag attttcctta tcttattata
    17101 attatttttt gtatcatcaa atctgataat agctccattt acatcaaaag taatcattga
    17161 gttttaacct ccccattagt ttcatcaata aaataaattt aatataaact ttataatcat
    17221 aatattaaac ttatttatac tataattatt aatttgatga taaaagctat tttccttcta
    17281 aataaaaaat ttgtctaaag tttctaaaaa atttagacaa atcttaggtt attactcttt
    17341 aaattatttt taaattattc caaatacgcc aaataatatt ggtataaata tagctggtaa
    17401 caaattggct acttttatat tcgttaattt aagtacattt agaccaagtc ctataattaa
    17461 taaacttcca gctgctgtca tttctgtgac tacaggatca cttaaaaatc catttaaaaa
    17521 tgatgctccc acacatataa taccttcata caaaaataca gatattgcag aaaacattac
    17581 acctatacct aaagaagcag taaatataat tgaagctatt ccatctataa cagattttac
    17641 aaataaagta tcatgacttc ctgtaagacc actttctaat gaaccaacta ctgccatggc
    17701 tcctatacag aataataaac tagatgatat aaaaccctct gctatagaat cttttttatt
    17761 tcctttagaa aatttacttt gtaaaaaagc acccaactta tttacccatt tgtcaatatc
    17821 tattaattct cctatcagag cacctacagc tacagatatt attgtcacta atgtgttttt
    17881 tccttctagt gctcctgata taccaatgta caatacacat aaagcaattc cattcattat
    17941 agtatcgctt actctttgag gtaatcctcc ttttattata agacctacta tacatccacc
    18001 tgcaattgct agactatttg ctattactcc aaacattttt tctcccctcg attagctaat
    18061 tgaaatttat cctattatta aaacctaaac atctatactt tgtattggat attttaaata
    18121 ttccaaaaat tcttcaaaac cttttctaga tatatttaca gttttagtat ttatgttagg
    18181 atgaaaattt aagtaatctt ctttaattac atctttatct atgtaaagct caacttttct
    18241 ttctgtatca tttattaatg aaaaaggatt tacacttcca ggaagaagtt taagtacatt
    18301 gtatagtctt tcaggtgaag caaacgataa tctagaactt cctatcttat ctttaagtga
    18361 gtttaaatca acttgttttt ctcctttaac tacaataaga taatacttat ttcctttggc
    18421 atttcttaaa aataaatttt tacattgaca cccctttgtt attccatcta aaatgactaa
    18481 ttgctctgct gtatatacag cttcatgctc tactacatca tattcaacat ttaatttatc
    18541 caaaatatca tatattttag tttcattcat gctagattca ttttcatctt gttctttttt
    18601 attatttaaa tcactcatac ttttctcctt aaacacttca tcataatatc attgtgaggt
    18661 attattctac tatagctatt gctcttactg gagctccatc agcatcatta tacttaaatg
    18721 gtgctgctat aaatagaaat ttttctggaa cagtttctaa atttgtaaga ttttctatga
    18781 ttatttttcc tttttcaaat aaaatatgat gcacttcaaa tactgatgtg tcatatctat
    18841 ccactgatag catatctatt cctatgcctt ttatattagt attagctata tagtttgcag
    18901 cttcttttgt taatgtagga taacctacat agtactgctt tttatcccaa aattttgacc
    18961 aacctgattt aaatattata aaatcactat tttttatttc atcttcatat ttttttagat
    19021 attctaattc tatattctca tttccctttt caagttccaa tacataagct ttcccaacaa
    19081 aattttctat atccaactta tctagggtct cacctttagt atacatatgt cttggtgaat
    19141 ccatatgagt tccattgtga gaaaatacac taattaatgt ttcttgataa ccattttctt
    19201 caataatagc tactttcttg atatctggtc tattaggctc tgcatataca ggcatgtcat
    19261 tatgtgtcac atgagttaaa tcaaaaacct tcatctttat atacccccta tgtttttatt
    19321 ttctattata tacctaaatt cattttattt ctatcagatt ttattccatt atataccata
    19381 ctactatata ttttttataa acctcaaggg atttaccgaa aaatcttgga aagaattatc
    19441 agtaaatata acatcttcac ccaaaaaacg tttatacctt ccttcttctt tctttaaagt
    19501 aattctctgt ttttgtgaat gattattaaa aaccattatc ttaagaggag gtttttttct
    19561 attagtctgc actcttttta acatgtattt tatgtgcata tcagcttcag gaaatgaata
    19621 tccacaaaat attaaaaggt cagtatctct taaagttttt tctgtttcat tccaaacatt
    19681 acttaaaaat atgtttgaca tattcttaaa atatgttggt ggcactataa taggttctgt
    19741 taactcacca cactctaaac attttgtctc tgaactattt tcaataagct tcatgacacc
    19801 accttcatgt ggtgtaagag ttacactatt acaaatgggg caataaagcc aattcaatga
    19861 cccatgtatc ttatataatt tgattaatgg ttcttttggt tttctccaat ttaactcctc
    19921 tttaaaattt gtaaactcca caccataatc aagcataatt ggcaaatcat tcttatctct
    19981 taatccagta actgtattgt ctatatgtat atcataattt gcactaataa aagtagtatc
    20041 aagaagcaaa tcattttcca aaagattttc aactaaaagt ttatgatatt tattatctgt
    20101 tctaggtgta ttatgaattg cttcagccat tagtagtatt aaatattgtc ttaaaaaacg
    20161 tatactatca cttctgttat ttagattttc tagtccaaaa tttctaaatg cttcttttct
    20221 ttgttctgct aagtctaaaa gacctaaaac ttcttcaaag gtaggaaaat ttgcattatc
    20281 tatattatct tttactatat caatattgaa catctgttta aagaaaatat acaattctct
    20341 attcattttg gtattataat ttgtgggatt tagtttttta aaatattcgc taaaaagttc
    20401 attttgaata ggtagattct ctgctgctga agctcctgct cctaaaaata ttgctatttt
    20461 catatagttc ctcctaatat aaatattact cctattttta tatccattaa atatccatat
    20521 catttatttt atgatacaac aataaagctg tcttttacta gaaaaaatcc tattacaaga
    20581 cagccttata ttatattaaa tctttaataa ttcgtaaata gaaagagcat atatttgagt
    20641 agcttttata atatcctcta ctattaagta ttcattagct tggtgttctg tttctggttt
    20701 tcctggaaaa atagctccaa atgcaacaca attatctaaa gctcttgcgt atgttgctcc
    20761 tccagatgaa agtggtgttc catcaagacc agtttcttct tcatatactt ttctaagtgt
    20821 cttaacaaga agagtatctt ctggaacata gattgaatcc aagaaatcat attcttcata
    20881 atttaaattg tatttatctg tcattttctt taactctttt acaaaatcat cctttttgta
    20941 tgttactgga atccttacat ctattcctgc aaattctttt tcgttatcaa tagttactct
    21001 acctatattt actgttagtt tacctgatac atcatccttg caattaggaa ttatattgtt
    21061 tccattagca tcttcaccaa taacttctgc taaaaactta attatattag aatctatacc
    21121 tatattatta agagctatac aaagtctagc cactgcattt ataccagtat cacttgctgc
    21181 tgaatggaca ctctttccaa ttatacagat tttattacct tcaacagtat attcaaaatt
    21241 taatttatca agttcttttt ttaatttatc agagtatttt ccaatataga ttgctttacc
    21301 tggaactgca tttaaagcct ttccaactga caactctata tcacttttgc tatcacaagt
    21361 caaatgaact tgtaaaagac ctttctctgc atttgttatt gggaatctag aatctggagt
    21421 aaatccatag tttggtattt cttcattatt ttctttatat ttatttatac atctccaaag
    21481 attttcttca tcagccccaa aaatgaatct tatcctctta ttaaagtcta catttaaatc
    21541 cataagagct ttcactgcat aaattgctgc tattgtaggt cctttatcat cttgtgttcc
    21601 tcttccatac aatttaccat cttctaaaac agcttcaaat ggagggtaat tccagctttc
    21661 taaatcacct tctggaacca catcaacgtg accaagtata cctatcattt tttcaccttg
    21721 tcctatttct gcatatccat aatatccatc tttgtatact gtcttaaatc ctaaagattt
    21781 acataagtct aagatacctc ttaaagcttt atctacattt tcaccaaatg gacaattttc
    21841 agttgcttca gatataacac taggtatttt tacactttct tgtattgaag aaatcatttc
    21901 atcttgtaga gaatttactt tttctttaat ttgctctttc atattatcac ccttcaattt
    21961 tcatcataat atactaataa atattttgac ctttaaaaaa aaaaatatgc ttattttttc
    22021 agttttttta taaattcaat cgcttttaaa gtccccattt ttgaatggtc aaatgtaagt
    22081 ccaccttgaa aataaacatt ataaggaggt cttattggag catctgcact taattctata
    22141 gaagaacctt gtataaatgc tcctgctgcc attatgactt cactttcgta tccaggcatg
    22201 tcccaaggaa caggttttac ataagaatcg actggtgcag cttcttgtat accttcacaa
    22261 aaacttatta cttcatcagc attattaagt ctaatacatt gaataatatc acttcttaag
    22321 tcatcatatt ttggtaatac atcatatcct aatttttcaa aagctcttga gcaaaagata
    22381 gctcccatta cagcctgaga tactatataa ggcgccaaga aaaaaccctg aagtacattt
    22441 ctagttgttc caaaagtaag accacattct tttcctattc caggagaagt cattctataa
    22501 gatataagct caatcaaatc ttttctacca gctatatatc cacctgtcaa tgcaagccca
    22561 ccaccaggat tttttattaa agagccagcc ataacatctg ctccaacatc agtaggctct
    22621 ttggtatcta aaaactcacc ataacaatta tcaaccatta ctatagcttc tggtttaaca
    22681 gactttatta cttctatagc ttcttttata tcactaattg aaagtgattt tctccaagaa
    22741 tatccttttg acctttgtat cataacgagt ttagttctat catttatttt gtttttaatt
    22801 ccttctaaat ctaagtttcc atcctctaaa aaatctacat catcatatgt aacaccatac
    22861 tctttcaatg aacctttttc ttctcttatg cctataactc cctctaaagt atcatatggt
    22921 ctaccagtaa cagataaaat ttcatctcct ggtctaacta taccttgtat acataaagta
    22981 agtgcatgtg taccatttac aattattggt cttacaagag catcttctgt attaaatact
    23041 ttagaataaa tttcctctat tttttcacga ccaatatcat tatatccata tcctgtagtc
    23101 cagttaaagt gcatatcact tagtttagat tcttgcatag cttttaaaac tttatattga
    23161 ttatattctc ttatttcttt tatttcttca aacttatctt ttatttcttc cattatttct
    23221 tgcgatagtt taaaagtatc atcatctatt ccatagtagt cttttaataa ttcttttgtc
    23281 tcattaagca ttttatcacc tgtctcttta ttattttatt taaacatgta aaggagacta
    23341 ttttactaaa acaaataaat tatagtttat aagttagtat aagtctcctc tcaatcatct
    23401 taaattaaat taattatcta tctgttgtta ttattattgt tgttttggtt ttgatttgtt
    23461 agattaatat actttccagg ttgtatagtt gatacagcat gtttataaat catgttttgt
    23521 tgtctattat ccccttctat taatattata taactatcaa acccctttac aagcccttta
    23581 acttgcactc cattaactaa atatatagta acaggtatcc tttctttcct tgcattattt
    23641 aaaaacaaat cttgtaaatt taaaactgta tttttcatta gatttgtacc ctcccaagat
    23701 taaattgaac ttctaaaata ttcctacctt atattctata taagttatga caaattttca
    23761 atattaagtt gtatacttaa aaatttatat attattttat agaatcttta atatataaga
    23821 ttatttcatt ttttaattcg tctatatttt catattggtc taaatcaaac cattttgctg
    23881 ttttatatct tttaaaccat gttatctgac gttttgcata ccttctagaa tctcttttaa
    23941 ttatttcaat agcttcttcg tatgtatatt ctccatccag atactttaat atctctttat
    24001 aacctatacc ttgcattgat atcatatctt ttttaaatcc catacttaaa agttttttta
    24061 cctcttcaac caatccattt ttaagcataa tatcaactct catatttatt ctttgataca
    24121 aatgttctct atctctattt agaactatta ttattggttg atattcttca ttaaatttta
    24181 aatcacttga aaaatcattc attttcttac cacttagaca aacttcaaga gctcttatga
    24241 cccttttagt attgttttta tggattctat tagcagattc ttcatcaagt tcttttaact
    24301 tattatgcat gtaatctatt cccttttcag caagctgttt ttgaagttct tctcttaatt
    24361 cattattggc atcagattgt gcaaaatcca tattatatat taaagagttt aaataaagtc
    24421 ctgtgcctcc agttactaaa acacattttt ccttttcatt tatttcatgt atatattttt
    24481 ttgctctttg ttgaaactct gaaactgaaa atggatagtc aggcttaact tcatctataa
    24541 ggtaatgctt cactccttgc atctcttcct ctgtaacttt tgcacttcca atatccatat
    24601 attcatatat ttgcatagaa tcagctgaaa ttatctctgc attcatatct tttgctaatt
    24661 ttattgacaa atctgttttt ccaacagcag ttggacctgt aagtattata agtggtattt
    24721 ttttcataac attcctttcc atatatttta aatatccaaa tattttacat aattctttta
    24781 aacatttttt ctatttctgt cttagatatt tcgaccatta taggtcttcc atgaggacat
    24841 gtaaatggat tctcacactt ttctagctgt tccaataaac tttttatttc aatatcgtat
    24901 attttatcat ttgcttttat agcagaccta catgccatag atgcaaatct ctctcctttt
    24961 aaatcataat tacttgtaat ttcttctata ttatcaatta tttgaagtat aaatttctca
    25021 gtttcaggta ctccaaatat agtaggaaca catcttacca ttatatgatt attgccaaaa
    25081 atttctagct caaaaccaaa tttcataaat aactccaagt tattttcaat ttgaagcata
    25141 tctacatttg atacttcaat aaccactggg tctaaaagta tttgcatatt tatatcttgt
    25201 ctatagaact tctccatata tctttcatat agtattcttt catgtgcagc atgttggtcc
    25261 aacaaataca tagaatcgtc tttgctcaaa attatataag tattaaatac aactccaatt
    25321 acagaatatc cgtataaaga aaacttgcgt tttgaactac acgagtattc ttcttcactg
    25381 tccttaatac tatctcctaa gtaataattt ccttcatttt taattccatc aacttgaaac
    25441 tcttcttgta tattatcatt tgcactatct tcacttagat aattaatgtt ttttacatca
    25501 tcttctacac ttgcatttag gacttcactt actgattgaa attcatttat tggtttttca
    25561 cttgataaag taacaacttc aatgacttca tccttacctt tagtaatatt actcttttta
    25621 ggagtagatt ctaataaatc attatttctt aatttataat ctgtactttt ttctctactg
    25681 ttaatagcga ttctaggttg tgttttttta tcactataag tagcatactt tccaataaga
    25741 tttgagtgta ttaatttaac ctttaaaaaa tctctaagtt ctatatacac ttcttgttct
    25801 ttttcaaatt taatttcaag tttattgggg tgtatattta catctataca tgatgggtct
    25861 acttctatat ttaagaagca cactgcatgt ttacctattg gaattataga cttataactt
    25921 tctgttattg catctatgat tattttactt tttacaaatc ttttatttat atagatatgt
    25981 tgtaagtttt tatttgaacg gtatatattg ttattaccta tatatccatt cattttaaag
    26041 tgattgcact taaactctac atctattata ttttccgtta tttcttttcc atatatactt
    26101 ctaatagtgt ttacaagttt tccatcccct ggagtattta gcatctgttt attgttattt
    26161 gtatatttaa attgaatgtt tggattacct attgcaagtt tatttatcaa atcacttatg
    26221 tttatagttt ctgcatgagt agattttaaa aatttctgtc ttgctggtgt attaaagaaa
    26281 atatctttta taatgatggt agttccatta gtagaaccta ttggttcttt cgatattatt
    26341 tttccacctt caacatatat tttagttcct attatttcat ctttagtttt agtcgtcatt
    26401 tctaattttg atacagctga aatagatgcc aatgcttctc ctctaaaacc aagagagtat
    26461 aaatcataca aatcatcaat ttttttaatt ttactagtag catgtctcaa aaatgatttt
    26521 tcaacttcac ttgaaggtat tcctattcca ttatctgtaa tcttaattag actttttcct
    26581 ccatcaatta tatctatgga aattttattt gcacctgcat caatggagtt ttcaataagt
    26641 tcttttacta cagaagaagg tctttctaca acttcacctg ctgctatctt atttatagtc
    26701 aaatcatcta ggatgttaat tatatttttc atttttacac ctctaagatt ttatttcttt
    26761 tgcttttctt tgaagattat aaagagagtt tatagcatct aatggagtca tatttaaaat
    26821 atccagattt aaaatttctt ctgatagtat atctctattt acactatcaa aagagatttg
    26881 agttaaggct acttctttta cagaatcctc ttgattaatt ttctctttat catcattttg
    26941 cttcattaaa gcaacttcat catttaatgc tttaaattta gaatttaatt tatcataatc
    27001 cttttttata tgtttgtaat cttttactat aagttcatgt tctattttta gagattcata
    27061 tttattctta aaattacttt gatttacctt aactaattct tcgtccaaat tactattaat
    27121 tatattattg ttttctttat ctccatttat tgcaacactg ttgtaaacat gatttttttc
    27181 caagtcttta agtatatatt ttgctctttc tatgacttcg tctggtaatt tagctaattt
    27241 agcaacatat attccataac tcttgtctgc tccctgagga attatttttc ttaaaaatat
    27301 tattccttcc ccatcttctt tgactgctat tgaataattt ttaacctctt taaactcttc
    27361 ttctaaatca gtaagttcat gataatgtgt tgcaaataat gtcttacatc taatattttt
    27421 ttgaatatat tccactattg accatgctaa acttatacca tcataggtac tagttccacg
    27481 acctatttca tctaatataa ctaaacttct ctctgttgca ttttttaata taagggatac
    27541 ttcgttcatc tctaccataa aagtagattg accttgagat aaatcgtcac tagctccaac
    27601 tcttgtaaat attctatcta atattggaat atcagcagac tctgctggta caaatgagcc
    27661 tatatgtgcc ataagagcta ttatagcagt ttgcctcata tatgttgatt tacctgacat
    27721 attaggacca gttattatgt taataatgtt ttctccacga tttaaataag tatcatttgg
    27781 aacgaaattt tcctctccta ctatattttc gacaacagga tgtctaccat ttcttatatc
    27841 aagtttatta ttttcattta ttgctggttt tacgtagtta tttatatgtg caactgttgc
    27901 aagggacaca aaaacatcta tattagctat agtcttagcc actttttgta ttctatctat
    27961 gtttttatat attgtatctc ttatctctac aaatatctcg tattctaaag atttaatctt
    28021 ttcctctgca tgtagtatct tttcttctat ttcctttaat tctggagtta tatatctttc
    28081 tgcattacta agtgtttgct ttcttatata tgtctcatct agctttgcct gtttaaagtt
    28141 agcttttgtt atttctatat aatatccaaa aactttatta aagcctattt ttaatgattt
    28201 aactcctgtt ttttctcttt ccctgttttc tatttccttt acaagaaagg ctccattctt
    28261 agaaatctct cttagttcct taagttcatc actaaaatct gatttaatta tgtttccatc
    28321 ttttatggtt atcgtaggtt cttctagaat agcttcatct attaaattgt atatatcatc
    28381 taatttatcc atttctgaaa catactcttt taggattttt gcatttgata aatttatagt
    28441 atcttttaaa ttaggtaact tttctatcga attttttaag tgaattaact cttttggtgt
    28501 tactctctca aatgctatct ttccacatat tctttctata tcgtatatat ttttaagtat
    28561 atcatttagg tcttctctta atataaagtc atcttttatt tcttctatta catctaatct
    28621 attttcaatt ttagatttat ttatcaaagg ttcttctaca tattttctta aaagtctacc
    28681 tcccattgct gtagaagttt tatcaagtac gtgtaataat gaaccttttt tcttattccc
    28741 tcttatagtt tgagtaagtt ctaaattagt tctagtaaac atatctaaaa ccatatattc
    28801 tgatgaattg tatatattta tattattaat gttagatgtt atttgttttt gagtattgta
    28861 tatataattc agaagaattg ataagcttga ctttattaga cctttatcat caaactttag
    28921 tttttgtaga taaacatctg gaaaatattc ttttaatata tttatatcca aatagttatc
    28981 actaaaactt tcatttatgt atatattact tacagtagct atatctctaa gcttttctat
    29041 aaagtctaaa tcatttataa ttatttcagt tggatggatt tttgcaattt cttcaattac
    29101 cttatcttca tttaaacatg ttgcattagt ttctcctgta cttatatcaa cataagttag
    29161 acctatatta gtaccatcct tatacaaaga taataaataa ttattctttt tattttcaag
    29221 taaatttcca tctaaaactg taccaggagt gatgaccctt ataacctctc ttctaacaat
    29281 tccttttgct gtagatgggt cttccatttg ttcacctatt gctactttat atccattttc
    29341 aactaatttt gatatatatg aattggctga gtggaaagga acaccacaca taggagcacg
    29401 ttcttctaaa ccacaagctt ttcctgtcaa tgctatttca agagcttttg aagctactaa
    29461 tgcatcttca aaaaacattt cataaaaatc tccaagtcta aaaaacagta tacagtcttt
    29521 atatctattt tttacttcaa gatactgttt catcatagga gttaatttat tcatatctat
    29581 ttgcattatc aaacctcctt cttttctaat taaatattat atcatagtag atgattatta
    29641 ttaacacaat atataaaatt ttgacatata actaaaaaca atccaaaatg tatactcaca
    29701 ttttggattg ctaagtttaa tgcttattaa ataaaaatat ttttttttac aataaagata
    29761 ttatttcttt tttaagttta atgatatttt caaggttatc ttcatttaac tttctactat
    29821 ctataacaaa ttctttcttt actattgatg gtttttttga aataacataa attctgtttg
    29881 atagcattat agcttcgtca atatcatgag ttataagaag tattgttgaa tttacttttt
    29941 tcttaacatc taaaagccat ttttgcatgg atgttttagt taatgaatca agtgccccaa
    30001 aaggctcatc taaaagcatt atgtcattag aatttataaa agttctcaag aagttagccc
    30061 tctgtctcat tcctccagaa agttctgaag gatatttatc ttcatatccg cttaatccaa
    30121 acacctcaaa ataaggtttt accattgacc tagctttact ctttttttct ttttttaaaa
    30181 ttagtggtag tgacacatta tctattatag ttttatatgg caagagtaaa tctttttgat
    30241 acatataact atacttacca tttatattca ctttgccact atcataatct gtaagtttag
    30301 ttataatatt aaaaatagta ctttttccac atccacttgg ccctaatata gaaacaaact
    30361 caccttcata aacatcaata cttatatctt ttaaaacttt aatatcttta taagtttttg
    30421 atacattttc tatagatact tttacttttt tattgtggta aaaattcatt tgtgtaagca
    30481 tcacttgcct tcatatcctt atctattaat ttatattctt ttaagaatga tgtatagttg
    30541 ttccatacac tatctttcat ttctccccat cgtggtgcat catctttgta cttgcttgct
    30601 aaatattctt gactctttaa agctagtttt tcatctactt caggtgcatg ttttactaaa
    30661 atttttgctg attcttctgg attttttatt gcatattcat aaccttttgt tgtagctttt
    30721 aagaattttt tagcaagctc tggattatct ttaagtacag tttcattaga aattataagt
    30781 ggtgtataat aatccaatct ctcatctaaa tcttttactg gtataaagtt taagtcatat
    30841 ccaattaaat cagctttaac tgcatcccaa ccttcaaata tccatgcaaa atctacagtt
    30901 ttcatagctg caaagaaatc atcttgacca gtattaacta tctttaactt attaaaatca
    30961 gctccatttt tcttcataac tgctttaaaa acagcttctt ctgatggaga gccccaacca
    31021 ccataagttt ttccttcaaa atctttagct gttgttatat tcttttcttt tggtgatgca
    31081 aacccagaag tattatgttg tataacagtt gctacagctt ttattggaag tggatcttca
    31141 ctagtctttg cataagtaac ttgttcttga tagcttatac caaagtcagc ttttcctgtt
    31201 gctactaaag tttctgctcc actttcaggt ggttgtacta tttcaacatc caaaccttct
    31261 tctttatagt aacccttatc aagtgctaca aataatccag tatgatttgt atttggtgtc
    31321 caatcaagca ccatagttac ttttttagaa gaagtatctt tatcattgtt tttgtcttta
    31381 ttgttatttg aagaacatcc agttattgtt gccaatgtaa aaacacttac taaacctaat
    31441 attcctaact ttttaaaatt cataattgcc tccctcaaat acctttaatc tattaatcta
    31501 tgtcttataa caactttttt tacagcctgt acaattccta caaacattaa actaaatata
    31561 actactaata tagtagatgc aaatacttta tcaagtgcat atgcactttt tgacctaagc
    31621 atataaacac caagtccttt cgttccacct agccattctg caacagttgc tgccattaca
    31681 gcatacgttg cagatatttt aagtccagaa aaaaatttat ccattgccat aggaaattta
    31741 aggtgaatga atgtattaac tttatttgag tccatagtct taaataaatt taaataatct
    31801 ttatcaatat tctcaatgcc atctacaaaa cttattaata taggaaaaaa acaagtaagc
    31861 attaccatca atacttttgg cattgttcca aatccaaacc atatcataaa gagtggtgct
    31921 ataattattg taggtatcat ctgtgtaacc aacataatag gataaagaca tttttttatt
    31981 attgatacaa aatccatcaa tattccaatt accaatgata ttataattgc gactataaaa
    32041 ccaagtattg cttctcctat agtaaccaca gcatgcatgg ataaattttg ataatcttta
    32101 aaaaacacat taattatatc tactggtgtg ggtagtatat actgaggaac ttctaaaact
    32161 cctaccatag attgccaaag aataagtata aacaaaaatg ttgctattgg atatatacta
    32221 tctttgactt tatctatgat ttctaagttt ttcatctata gtcagacctc cagctttttc
    32281 atctactcta gctactgtta tgactctttc acatccaaga ttaaaaggga ttttatgtac
    32341 cttttttaat aattcaaaaa cttcatcaaa atcaccttca acagtagtgc tcattgctcc
    32401 tatttcacat ttaagaccac ttttttcaat ttcagcaata caagcatcta ctactttata
    32461 catctcttct tcacctttgt aaggtcttag tggcattact gctatgtctg cgattaccat
    32521 ttaaatcact cctttaaaaa taattaatac ctactaggta tattttgttt tttattaaaa
    32581 aattttatta ctaaaaagat attatattta aataaaagaa aagtcttatc caaaatagga
    32641 taagacttat ttttatctta actggtctcc ctacactggc attatccaaa caggttcaaa
    32701 gggtcgagat aaaatctcct ctcagcacat tagctccccg aaatatatta tatcttttct
    32761 acttgattat actacactta tcatatttta acaaattttt tatttatatt agatatttta
    32821 tccgttcaaa aatatctaat ataaattgta cattattaca ccaaataaat tatcttaaaa
    32881 tgctaattta tcaacatctt ccaaaactag attaactgaa ttttctagtt tttattttgc
    32941 tatttcaagc atatttggat gtatatcaat tccatacaaa tattgtgcta aatttctgtt
    33001 ttcactcatc taatttgcaa tcgttacaga ttacagatta gaaatagctt ttagactcta
    33061 cttatcttta tctacaaaaa atccttcaat aaattcagta ataatattca gtaatctaaa
    33121 aataaacctc tatctagatt tttaagatac aagcctttta ataagtacct tttgtgataa
    33181 tataaaaatt actcgtcatt atcatcaaaa tcctcagcaa ccaacatttt agataagaat
    33241 caaactctat aatataatta ttttatccaa tttatttttg agaaataaaa tattttttta
    33301 atttttaaat tttttaatat gcaccttgaa aaaagaaacg ttttcctata ttatggtaat
    33361 agtagaataa aactaaatag gcaaaactag agaaatttag tgacgcaaag ctatagggac
    33421 taagacttat gaaatgagtt atgtcagcca gttgccaaag agatattttc tttttgtttt
    33481 tatgaatgtt ttttaggggg atatatgata tatgtttatg gataaaatgg atagatgtac
    33541 acatatacta actgcatata tatgcagttc acgcgattac tgcaatttta tagatacaca
    33601 attaaatgat tttatactag aatatggaga gaatgtggta gaatcttgtt tgcatcaagt
    33661 gatggtattg gtaagcagat ataattaaag aaattttatt ttctagaaaa ccctttttag
    33721 ttttatctac gttttataat tcattaaaga actaattaaa ataagcttca ttatatattt
    33781 ttcatgaagt aaacagttga gtttatctca tatactgttt accccaatta cataattcta
    33841 ataatataga ttccaatgat ttaccacgtt tagtagacaa atattcaact tttggaggaa
    33901 tagttggata agaaatcctc ttaataagtc aatctctttc aaaatacttt aattcttgtg
    33961 acagtatctt atgacttata tgaacaactt ctttttttaa ttgactgtaa cgtattggct
    34021 cttgttttaa aatatgccat aaaataattg ccctccattt acttcctatc acagtcatgg
    34081 taaaaccaat tgaacaatta aaatcttttt gtctttcttc aatatttgga aattcttcaa
    34141 aattattatc tatatccatc tcaatcctat ctttcaaaat agtaagtatc taactaaaaa
    34201 gtgcattctt gtttagcgta ctaattcgta atataatctt gattgtagta tagagcctta
    34261 cataatacta cttttttaga tgtatatgtt taaattttaa tatagacata ggttatttta
    34321 ttaattttat ttaaacagat aataataaat aataatttat ctcaagtttt ctatgtagat
    34381 aaactaatct aatgtttaga aaggattata taatatgaat aaaaaatttc gtctttatca
    34441 agttgactca tttacaaagg aaaaatttac aggtaatcca gctggtgtaa ttacaaatgc
    34501 tgatggctta tctgaatcac aaatgcaaaa aatagcaaga gaattaaata attcagaaac
    34561 tgcttttatt ttcaataact tggctaaaaa tcatcaagaa aattatgatg tacatatcag
    34621 attttttaca ccaactaatg aggttcctat atgtggtcat gctactattg ctgcccatta
    34681 tgttagagca gtagaaaacc aattcaataa caaaacagta tttcaaaaaa ctggtgctgg
    34741 aatattacca gttgatatta taaaaaaaga taatgattat gaaataatta tgacacaagg
    34801 aaaaattgaa gtacctccac cattacaaca aacctatcaa aaaagattac ttaatgcatt
    34861 tggtatttta aagaaagata ttcgtacaga ttgtcctatt gccatatcat ctacaggaca
    34921 ttcaaaagtt atgattggaa taaaggaatt atcaacatta aatagtttat ctccaaattt
    34981 atatgaatta tcgaaaataa gtcaagaaat aaaatgtaat ggacattata tttttacatt
    35041 acataataaa gaaactctac aagttcatgg aagaatgttt gctcctgcaa ttggtatttc
    35101 agaagaccct gttacaggaa atgctaatgg acccttaggt gcgtaccttg tacattataa
    35161 agtaatacct acacccaaca cagagtttat atttgaagca gttcaaggag aagcaataaa
    35221 acgcaaaggc aagatgttag taaaagtaat tattttagat aataagccag ttgaagtaaa
    35281 aataattgga aaagctgtaa ttgcattctc aacaaacata gaaatctaat tatagattcc
    35341 ttctaagtaa atttttgaac tttttaatac taatttaata acaaactaat ttatatacaa
    35401 tcaaattaat ttttttatta tttgttaata tatatttatc a
//
LOCUS       DAKBNN010000015        34682 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000015 DAKBNN010000000
VERSION     DAKBNN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.015"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..416
                     /locus_tag="OUB02_000757"
     CDS             <1..416
                     /locus_tag="OUB02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8884550.1"
                     /translation="LKSKEEVLNFIADSAYILGITTNKEALLKDLLKRENEFPTGIKD
                     GFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAPHKNEGNIHLKTLS
                     KLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            419..727
                     /locus_tag="OUB02_000758"
     CDS             419..727
                     /locus_tag="OUB02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCT8884551.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            841..1947
                     /locus_tag="OUB02_000759"
     CDS             841..1947
                     /locus_tag="OUB02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCT8884552.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENIEFDEIELDIM"
     gene            2022..4700
                     /gene="mngB"
                     /locus_tag="OUB02_000760"
     CDS             2022..4700
                     /gene="mngB"
                     /locus_tag="OUB02_000760"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCT8884553.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5017..7350)
                     /locus_tag="OUB02_000761"
     CDS             complement(5017..7350)
                     /locus_tag="OUB02_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8884554.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKAISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTMTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7325..8041)
                     /locus_tag="OUB02_000762"
     CDS             complement(7325..8041)
                     /locus_tag="OUB02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884555.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(8456..9112)
                     /locus_tag="OUB02_000763"
     CDS             complement(8456..9112)
                     /locus_tag="OUB02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8884556.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILAEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(9167..9796)
                     /locus_tag="OUB02_000764"
     CDS             complement(9167..9796)
                     /locus_tag="OUB02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCT8884557.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(9927..10568)
                     /locus_tag="OUB02_000765"
     CDS             complement(9927..10568)
                     /locus_tag="OUB02_000765"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCT8884558.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(10577..11248)
                     /locus_tag="OUB02_000766"
     CDS             complement(10577..11248)
                     /locus_tag="OUB02_000766"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCT8884559.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANIIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(11253..12137)
                     /gene="rsgA"
                     /locus_tag="OUB02_000767"
     CDS             complement(11253..12137)
                     /gene="rsgA"
                     /locus_tag="OUB02_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCT8884560.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLVLEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(12235..12972)
                     /locus_tag="OUB02_000768"
     CDS             complement(12235..12972)
                     /locus_tag="OUB02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT8884561.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(13080..15095)
                     /gene="pknB"
                     /locus_tag="OUB02_000769"
     CDS             complement(13080..15095)
                     /gene="pknB"
                     /locus_tag="OUB02_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCT8884562.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(15112..15864)
                     /locus_tag="OUB02_000770"
     CDS             complement(15112..15864)
                     /locus_tag="OUB02_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCT8884563.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(15884..16915)
                     /gene="rlmN"
                     /locus_tag="OUB02_000771"
     CDS             complement(15884..16915)
                     /gene="rlmN"
                     /locus_tag="OUB02_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCT8884564.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(16923..18248)
                     /gene="rsmB"
                     /locus_tag="OUB02_000772"
     CDS             complement(16923..18248)
                     /gene="rsmB"
                     /locus_tag="OUB02_000772"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCT8884565.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFILDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQGVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(18310..19011)
                     /locus_tag="OUB02_000773"
     CDS             complement(18310..19011)
                     /locus_tag="OUB02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCT8884566.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(19080..19832)
                     /locus_tag="OUB02_000774"
     CDS             complement(19080..19832)
                     /locus_tag="OUB02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HCT8884567.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(19842..20771)
                     /locus_tag="OUB02_000775"
     CDS             complement(19842..20771)
                     /locus_tag="OUB02_000775"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCT8884568.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(20786..21226)
                     /gene="def"
                     /locus_tag="OUB02_000776"
     CDS             complement(20786..21226)
                     /gene="def"
                     /locus_tag="OUB02_000776"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCT8884569.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEDLIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(21244..23769)
                     /gene="priA"
                     /locus_tag="OUB02_000777"
     CDS             complement(21244..23769)
                     /gene="priA"
                     /locus_tag="OUB02_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCT8884570.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(24008..25207)
                     /gene="coaBC"
                     /locus_tag="OUB02_000778"
     CDS             complement(24008..25207)
                     /gene="coaBC"
                     /locus_tag="OUB02_000778"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCT8884571.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(25212..25478)
                     /locus_tag="OUB02_000779"
     CDS             complement(25212..25478)
                     /locus_tag="OUB02_000779"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCT8884572.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(25481..26098)
                     /gene="gmk"
                     /locus_tag="OUB02_000780"
     CDS             complement(25481..26098)
                     /gene="gmk"
                     /locus_tag="OUB02_000780"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCT8884573.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(26128..27009)
                     /locus_tag="OUB02_000781"
     CDS             complement(26128..27009)
                     /locus_tag="OUB02_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCT8884574.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(27013..27843)
                     /locus_tag="OUB02_000782"
     CDS             complement(27013..27843)
                     /locus_tag="OUB02_000782"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCT8884575.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(28130..29164)
                     /gene="ltaE"
                     /locus_tag="OUB02_000783"
     CDS             complement(28130..29164)
                     /gene="ltaE"
                     /locus_tag="OUB02_000783"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCT8884576.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            29460..31235
                     /locus_tag="OUB02_000784"
     CDS             29460..31235
                     /locus_tag="OUB02_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCT8884577.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSRFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(31450..33102)
                     /locus_tag="OUB02_000785"
     CDS             complement(31450..33102)
                     /locus_tag="OUB02_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT8884578.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCGHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     EGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEIKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEKKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(33401..34612)
                     /locus_tag="OUB02_000786"
     CDS             complement(33401..34612)
                     /locus_tag="OUB02_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCT8884579.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
BASE COUNT        11369 a         6077 c         3844 g        13392 t
ORIGIN      
        1 acttaaaaag caaagaagaa gtattaaatt ttattgcaga tagtgcttat atactaggta
       61 ttacaactaa taaagaggct cttttaaaag accttctaaa aagagaaaat gaatttccaa
      121 caggaataaa agatggattt gcgatacctc atacaaagag tgtaaatgta aaggaagctt
      181 ccatactcta tattaaaaca actaacgagt tagagtggaa aactttagat gatagtaagg
      241 ttagatatgt atttagtttg ttagccccac ataaaaatga aggaaatata catttgaaaa
      301 ctctatctaa attagccaca tgtttaatgg aggatgattt tacaagtgaa gttaaaaatg
      361 ctagtgatgc aagtcattta gtaaaatata taactaataa aatgaaggag gaataattat
      421 gaaaatagta gcaataacat catgtatagc aggattagca cacacaccta tggcagcaaa
      481 agctttagaa caagctgcat caaaaatggg tcatgaaatc aaagtagaac aacaaggtgc
      541 tatgggacga gtaaatacta taacaaaaga agaagcacaa gatgcggact ttatacttat
      601 agcatctgac caaactatat ctggaatgga aagatttgat ggtaaaaaag tgataaaagt
      661 caaaataggt atagcgttaa aaaaaccaga agctgtctta acaaagtgta tagaagctat
      721 ttcttaataa aaaaatctca ctctaatata gaatgagaaa aatcaaaaat taaaaaattg
      781 tctaatataa ttataacata ttatcaaaaa atataacaaa cttatatagg aggattaaaa
      841 atgagtaaaa aaaataaatc tcttttaagt gaacttaagg gacacttaat gacaggtatc
      901 tcatatatgt tgccacttgt aataggctca tctttagttg tagctatccc aaagcttaca
      961 gctttagcag tgggtataac aagtttagat gcttataaaa atgtatctgg tttttatcat
     1021 attctttact taatggaaca agttggttgg actggcatag gtctattaaa tacggtatta
     1081 gcaggattta tagcattttc aataggtgag aaaccagcta tgggtgcagg ttttattggt
     1141 gggctcatag caagtaatac taatgcagga ttcttaggag cagtaatagc aggtttcttt
     1201 gctgggtatg tttgtaagtt tttaaagaaa aaaattaaaa tatctgggtc agcatcagga
     1261 atgatgccac ttataataat gccacttata acagttggat taactggttt tttaatgagt
     1321 gttgtattag caggaccttt aggaaacata aatacttcat taaatgcttg ggtagctgaa
     1381 atgtgtcaaa atggaacaaa ttcggtggta ttggctttaa ttttaggcgc aatgatagga
     1441 ttcgatttag gtggaccagt aaataaagca gcttggatgg caggaaatgc attattgtta
     1501 gagggaatct atctaccagc gatattggta aactgcgcaa tagttatacc tccactagga
     1561 tatggtttag ctacattcat aagaaaatca aaattttcca atgcattagc tgaaacagga
     1621 aaaggcaata tagttatggg tataattgga ataacagaag gtgcaattcc tttcacttta
     1681 acaaatccat taaaattagt tcctgttaat atgataggat gcgctttggg tgtaggatta
     1741 tcagctttgt taggtgtaca tgctataatg cctccagttg gtggattata tggatttata
     1801 tcaataggtt ctggatgggc ttacttagta ggtgcaatat ttggagcttt agtaatagca
     1861 atactatcaa caactttagt agacttcaat gaagatgaaa ctacggaaaa tatagaattt
     1921 gatgaaatag agttagatat aatgtaaatt aaaaacttaa ttttagataa ttgagaattg
     1981 agtgtactct caattctcaa ttaaattttg ggaggatttt tatgaaaaag acaaaagtac
     2041 atgtagttcc acacagtcat tgggatagag aatggtattt tacaacatca aggtcaaaaa
     2101 tatatttaat gaaagatttt caagatatat tagacatatt ggaagaaaaa gaagacttta
     2161 aatactttac tatggatgca caagcttctc tcttagatga ttatttaaaa tggagacctc
     2221 aggatgaata tagaataaga gatttagttc aaagaagaag attagttatt ggaccttggt
     2281 atactcaaac agaccaattg gttatatcag gagaatctat aattagaaac ctttactatg
     2341 gaatagatag atgcaatgaa tttggtgaac ctatgagggt tggatatgta ccagattctt
     2401 ttgggcaatc agctcaaatg ccacaaatat acagaggttt tggaatagat agttcattat
     2461 tttggcgtgg agtttctgat gatatggtag atacaacaga gtttatatgg aaaggttctg
     2521 atggttcaaa agtattagca gttcaaatac catttggtta ttactatgga ggtaatatac
     2581 cagaagatga ttcagattta aaagaatatt taaaagatat aatcggaaaa cttaaggtta
     2641 aagcttctac taataacgta tattttccaa atggatttga ccaagcacca ataagaaaaa
     2701 accttcctga tatattaaaa aaagctaatg aaatagataa tgaaaatgaa tacgaaataa
     2761 gtgatataga aaactatata gattcagtaa gaaatgaaag aaatgaattt acagagctta
     2821 aaggtgaatt tttaaatgct aaacatatga gaatacacaa atccatattc tcctcaaggt
     2881 cagaccttaa aataatgaat aacaaattag aaaattatat tacaaatgta cttgaaccat
     2941 tattaacatt aagttattca cttggaaatg aataccctca tctagttata aaagatatat
     3001 ggaaacttat gtttgaaaat gcagctcatg acagcatagg ttcttgtaat agtgacacaa
     3061 caaatgagga tgtatacaca agatacaaac aagccagaga tttgagtata aatttattag
     3121 atttgcatat gagacttata agtactcaaa tcaaaaataa taatgaagaa ataacattaa
     3181 cagtattcaa tggattccca gaagtcagaa atgaagttgt agaatttgat acttatattc
     3241 caggtcaaga ttttgtgata aagaataaaa atggagaaat tcttaattat ataataaaag
     3301 aaaaagaaga tattacaaat tatatattaa ctcaaacaat aagattaaac ccaagtaaaa
     3361 aaatatatat acccaaaact gtatatagag caaaaattgc actttcagta aaagatatac
     3421 caccaatggg atatactcaa ctagtttttg aattggatac taaaaataaa tgtgaaatag
     3481 aaacaagtaa ttgcaaagaa atagaaaaca aatactacaa aataacagcc aataaaaatg
     3541 gttcattaga aatattagat aaagaatcta ataagactta taaaaaccaa gctgtaattg
     3601 aagataatgg agatgatgga gattcataca actattctcc tccaagaaaa gatatttatg
     3661 tttcatcttt agattctgta tcttcagtag aagtgctaaa atcaaacatt caagaagaaa
     3721 tgatacttaa atattcattg accataccag cttcactgga agaaagggct ataggtaaag
     3781 tttctgtgaa aatgccagta acaatgaaaa ttaccttaga acctaaagaa caaataatta
     3841 agtttaatgt taatataaaa aataaccaag cactatctca tagagtaaga gttttattta
     3901 atacagaaat agcatctaag ttttctatag cagatcaaca atttggaata atacaaagac
     3961 caacagtatt agaaaaagac cttgcattgt ggaaaagaga caattactca tggcaagaaa
     4021 aacctataac tatcgaacca atgcaaagtt ttgttacttt agaggatgga caaagaggaa
     4081 tagcattaat aactggttgt gtaagagaat atcaaatagt tggtgataat ttagatacaa
     4141 tagcattaac actattcagg tcatttggat atatgggaag agaaaattta ctttatagac
     4201 caggaagagc ttctggagaa aaaataatag caacaccaga tgcacaatta cttaaagagt
     4261 taaactttga ctttggtctt tacatatata acaataaatt tgatgaagca aatgtagcta
     4321 atacagctaa gagattctta acacctatac aaatatatga atatgctgat tttttaaatg
     4381 gtagacttat atttgcattc aatgacgaaa aacaaatata tgaaaatgaa tatagtttga
     4441 tgaatgtaaa taattcaaat ttcacagtaa gtgcaataaa gaaagaagaa aaaggagatg
     4501 gaattgtagt tagaatcttc aatggtatga aaaatgagga tgctaaggga agtcttaaca
     4561 tcaataaaaa tgtcaatgat gcttatagtg caatgctcga cgaaatctat gataatacta
     4621 gcaagcttaa agtaaatact aaaactgtag aggtaaattc tttatcacat tgtaaagtac
     4681 aaacaatagt aattaaataa tataaaatag cttatacaag atttcaaaat aaattgctag
     4741 tactatgtta atagtaaacc cagatattat gctatttata agctctgtaa cactaaataa
     4801 cattttgtaa aataaaaagg tgaaccttgt atattaggat tcaccttttt taaataaatc
     4861 aacattctgt tttaaaataa catttataag ctgttttgaa ataatgctta caaattattt
     4921 taaaacaata cctataaatt tttatttaaa gaatacacat aaatatttcc agactctttt
     4981 attctataga ctttataatt attaaataag tctaatctaa tttttgttaa aagtaattgt
     5041 aactttaaat aaatcaccat ccacacttat attcatctta ccaccctgta attctataaa
     5101 actttttgct atcgctaaac caagaccaga accttctgtg ttacgtgatt tatctcctct
     5161 tacaaatctc tccattatat catctacact aaaatcaatc tcagatgcag acatattttt
     5221 aagcataata tttactttat tatctgtttc taaaatatca atatacactc ttgaatttgg
     5281 cattgcatat ttgcttatat ttattataag attttcaaat actctaaatg tacgctgact
     5341 atctaatggt aatattattt tttcattaga aaagttattc tttactatta gagatgaatc
     5401 tgcaagtttg tcatctaatt ctaaaagtgt ctgcttcatt agagatacaa tatccacatt
     5461 tactatattc aaataaacat ctccactatt tgccttgctt acttcaaaca aatcttctat
     5521 tagatgttgc aatctttgtg actttctgtc taatgtatca atatacaact tacgattttc
     5581 atctgttata ctttcatctt ttaataaatc tatataagtg attatagatg ttagaggtgt
     5641 ttttaagtca tgtgatacat ttgatataag ctctgttttc atcttttgac tcttaacctc
     5701 ttcgtcaaca gcttttttaa atccagtttg aatatttcct aattcgtctt ttataggatt
     5761 aaaaaatcct aaatcttttt ccatatttac atctaagttt ccatctgaaa ttttctttgt
     5821 aacatctaaa agttgattat attcatatat tgtcttttta gagtaatctg acagtatctt
     5881 aaataatact actgaatata ttaatgcgag tattattcca aagaaccaca tactacacat
     5941 tataactata atcaataaat ttattcccaa aattgtaact atagttttat ttggcttttc
     6001 ttttagattt attgttctaa tttgattaat aactgttttt cctgcttttt ttagccatac
     6061 aaaaaacatt cctgtcatag tatgttttgt aaaatactct cttattccaa catttaaaat
     6121 gtgttttaaa agtattataa atacaaatat tattgagaat attacaaacc aataagttaa
     6181 attcattaaa cttaccaata tgctagccat tttagcactt atgtcattgt ttataaggta
     6241 ctctacataa ttcccactca aggtattact tattataaca gtaggatata aatctacaag
     6301 cccctgtata atagcaagag caataaagag tatctctaac ggtattcttg ataatgtatc
     6361 aaatcctagc aactctttag attttctata tgggaatgct aaagctacaa taacaactat
     6421 agctaatata atttttaaaa tcattccaga aatcttataa taagaattat tttctacatt
     6481 gcttatatat gaaattatac catcatcaaa ataagtattt ccataaccat actctacact
     6541 atcaccttta cttattgctt taggtacagc atatactaat gttatatttt ttattggaga
     6601 taacttatat gaaattgaat ttcctcctaa acttgtatcc attaaatctt ctcttgcttt
     6661 agataacaac atttgattaa taactttttg atttactcca taagaatcta caatatttac
     6721 ttttccatct ttatcaaaat tcattacaac gtagaatcta tagttttctt ttatatctgt
     6781 aatctgcttt tgtctttcct catcattcat ttctgtattt tttgactgat tttcatcaga
     6841 attttctttt gaagcatcat tctttgttgt atcaagtact cctaattctt tattatcatt
     6901 aaaaaattca aaattgcttt ctttatcaac taaatagtac tttaaattct ttcctgacgc
     6961 tttaatgaaa tgctcccaat tttcaaagtt ttcattaaaa tctgcattaa tactatcatc
     7021 tgtatagtaa gtttctgaac catcataagt atctttttct ttacttacat tttttatata
     7081 gttcatagtt tcttcattga taaatagctt tgatggttgt atcatcttac cttcttgttc
     7141 ttccttcata ttatgatata aaacataact tcctttatat atactatcta caaagctttc
     7201 ctgttcaaat ggattctctg ctccaccttt agcacttgct tgtatagtag aatattgact
     7261 aatcatacct aatgatgctg ataacataag tataaaaact ataatagtaa ctatatattt
     7321 atctttaaat tttttcaatt ttgtatccaa caccccacac cacctttaaa tatttaggat
     7381 tttttgggtc aatttctatc ttttccctta tattcctcac atgtaccata actgtatctg
     7441 tatttacagc attctcattc caaactcttt catatatttc atcagcagaa aaaactcttc
     7501 ctgcatttct cataagtaaa cttagtattt taaattctat aggagttgtt tttatatact
     7561 ttccatcaat actaacttct tttgtatttt catttaattc taatccgcct attgcaaaca
     7621 cactatcgtc actttctacc ttttgctctt tatttccaac catgtttaga tacttagtgt
     7681 accttctaag ttgtgaatta actctagcca atagttctaa tggcttaaaa ggttttgtta
     7741 cataatcgtc tgctcctata ttaagcccca ttattttgtc aacttcctct gattttgcag
     7801 atagcattat tactggaaaa ttatagtttt ctctaagctt cattgtaagt gttataccat
     7861 ccattttagg catcattata tcaactatag ctaaatgtat ttcttggttt tctataacct
     7921 taagaccttc taatccattg tatcccttaa aaacattata tccttgattt aatagatata
     7981 tttctatagc atctgctatc tccttttcat cttctaccac caaaatatta tatgaattca
     8041 ttctaatctc ctttatatta atttataaat tttctttaca tatatgatta tacaatatga
     8101 tagaagtttt tccatttata tatatattta tatcttttgt tctggttatt tgtatttaat
     8161 gtccatctta gaaacctcct tctagtttaa atttaatatt ttttcatttt gttttatata
     8221 cttaatatta aaattaaaaa cttaagatat tacatatact tttctaaaga ttttctaaag
     8281 atttaaatat atttaaaatt aatatacaaa aaaaagtagt tcttattgta ttaattaaat
     8341 caatgatttg aaatataaaa ttaatatcaa taaaacctac tttttaattt tttaaatatt
     8401 tatttttaat ttattttgtt tttaatttac ttcgatttta aaatgtcctt gacttttatt
     8461 ttataaatgt acatatttct tctactggtt ttttagatgg actcttcaaa ttgtcctctg
     8521 gcactccaag agctaacatt gcacctaaaa aatactctcc cttttcaaaa ccagtttctg
     8581 cttctaaaac agcttctatc tcatctgctg catagttttg acttgtcaac caacaagaac
     8641 catatccaag ttcaattgct gatagtgtaa agttttcaat tgcagcacct agactctgca
     8701 tacctggatt tcttataaat aatttatcta tagtctcctt aggtgcatct ataagctcta
     8761 attcataata tcctgatggg ttatatacct ttgtaaaaac taatactaaa actggagctt
     8821 ttaaataaaa tagtgtaaag tttttaacaa attttctaaa tctattagcc ttatccactg
     8881 atactttatc catttctgca agtatttctt gttgcttttt agttatcaca tctgctatct
     8941 tttccattaa atcacggcgt tttataacaa caaaatgcca gttttgaata ttcttgcctg
     9001 atggtgctgc acctgctgct tttatcattt ttaaaatatc ttcatctgat acatcttgat
     9061 ttttaaactt tcttacactt tgtcttttaa aaatagtatc ttgtaattcc atttttaaat
     9121 ccccccataa cttttttatt attttaatat aatttttaaa ctcatcttat tttaatatat
     9181 catgttctaa caaatcttta aatgagtcta taaaataatc atatacatct acattcccaa
     9241 acccatattt ggcatatata aaaggtatac ctacaaactt agcactattt gcatcacctt
     9301 gcgtatcacc aacatagatt ggatttatta atttatttct ttcaattata agtttattat
     9361 tttctccttt atgcaaacca gtattaccag gacattcaaa atctataaaa tactgttcta
     9421 gtttatgtgc ttttaaaaaa gattctatat atccagcttg acaattactt actataaata
     9481 atttatattt tttagatagt aatttaagag tatcttctat atcatcaaat aatttagctc
     9541 cattttcaga taaatagtca cattctttca aagaacattc attcaataaa ttcattcttt
     9601 cttcttcact taaatttgga aataaacgat ttgctatttc ctcaaaagaa agccccataa
     9661 gacttctaaa taaatctttt gtgacactta attctatttc cttatgttta cttaaaacat
     9721 cctgccagac tttgcatact tcttcagttg aatcccacaa tgttccatct aaatcaaaaa
     9781 taatactatc taccacaaaa aatccccctt atgtcaaagt ttattatata tttataatta
     9841 tatactaaaa agcagataca atcataaata tattttactt gattgtatct gctctacaat
     9901 ataaaaccaa ttgtcaaaat caaattttat aaatttctaa ctacaagtac acagccttgc
     9961 tctactttaa tcttacaaga attactcaac ataacatttg atattccaat aggtacagag
    10021 tatttcatat aataattatc gagagggtat tctaagccag taagtgttac acccttagca
    10081 tcaccattta tagctattac agaaatagtg tcccctgggt ttccatacaa tgaaatttcc
    10141 tcattttcaa ctatatacat ctcttcttta tcagaaagta tcctagaata tataccatct
    10201 tcttttaaat aatataatag ctttatattt gcaagagtgt ggtcaatcct tccccctaaa
    10261 gcaccaaaaa aatcaatatg atttgcttta agtgtcttag ctaaaaaaag acaaagctct
    10321 gtatcagtct catctttttt agatggaaat ttttcaaatt taacaccttt atttttatag
    10381 aaatttatct tttcttcttc tactgaatct aaatctccta atatataatc tggcattatt
    10441 tccattttat aagtgtgatt tgcccctcca tctgcacata ttatgcagtc ataacattcc
    10501 ttaattataa tatctcttgt aacttcgtaa tcttcaattt cgccatttaa aacaatacag
    10561 attttcatta ttaaatttaa actaattctt caagagaagc attctttcta aataaattaa
    10621 ctgtttcttg aatgtcatca cttccaaata ttgcagaacc tgctactatt atattagctc
    10681 ctgcttgtac aacttcggct acgtttttag ggcttatacc accatcaact tctatatcaa
    10741 tgtttaatcc ttgactatct ataatagctt ttaactcttt aatcttatct aaaacacaag
    10801 gaataaatga ttgtcctcca aatccagggt taacagacat taataatacc atatctaaat
    10861 caggtagtac atgtttaatc gtatctactg gtgtagctgg attaagtact acaccagctt
    10921 ttattccatg tgattttata ttttgtatag ttctatgtag atgaacacaa gcctcctggt
    10981 gaacagttat tatatcacat cctgctttta caaattcttc aatatactgg tctggatttt
    11041 ctatcataag atgagcatca aataccatat ttatattttc tttctttaaa cttttgacta
    11101 caagtggtcc taatgtaatg tttggtacaa agtgtccatc cattacatcg atatgaagat
    11161 attcacatcc tgcactttct acttttctta catcttctaa taattttgca aaatctgctg
    11221 ataatattga tggtgctaat ttaatcatgt ttctaatacc tcctactatt attttgtctt
    11281 atttcatgta gcagctgtat ataactatca tatctttctt tagatatttc tccatttgta
    11341 acagcttctt ttatagcaca attaggttca ttttcatgaa tacactttga accaaactta
    11401 caatcattaa atttatcaaa ttctataaaa taatctttaa gttcaacttc ctctatatct
    11461 tcaaggacaa gggaactaaa acctggtgta tctgcaacca ttccaccaaa ttctaatttt
    11521 aaaagttcag cgtgacgagt agtatgtttt ccccttttta ttttatcact tacaactcct
    11581 gtttgaagtt taaaattttc atctatttca tttaataagc ttgattttcc aactccagat
    11641 ggtcctgcaa acactactac attttctttt aattcttctt ttactttatc tatatttaac
    11701 ttagtaatat tacttacagg aatgacttta tagccactaa gttcatatat atttttaaca
    11761 gtttctaatg tatcattatc atctaaatct gcttttgtaa ggattattac agtttctaaa
    11821 ttttcttttt ctgccaaaac tataaatcta tctaaaagag ataagtttgg tttaggattt
    11881 tttattgcga atactattaa tgctttatct acatttgcaa taggaggtct tataagttct
    11941 gtatctctac tgtcgatttc ttctaagata cccttttttt catcttcatc aacaatgctt
    12001 attttgactc tatcaccaac caaaggtgtt attttctgct ttctaaatat ccctctagcc
    12061 ttacattcat aaataccatt ataagtatct acataataaa atccactaat cccctttata
    12121 atttttcctt ctagcatcaa atgcctccct ttataaaatt tatataaaca catactgttt
    12181 atgagtagta tatcgtgtta ttctttccta tttttatata ataccacatt ttttttaaat
    12241 aacaaatcct ccgtttggac taattacctg accagtgata aaatttgatt tgtctgatgc
    12301 caagaaaata gcacaatttg ctatatcttc tggagttcct agcctcatta gaggggtttc
    12361 ttccacaaga gcgtctatat cttcctcatt atatccagat aacatgtcag tattaatgac
    12421 acctggagct atgctattta ctctaatatt tgatggtcct acttctttag ctaatgcctt
    12481 tgtcatacct attatgcctg cttttgttat agaataatgc acttcacaag aggcacctga
    12541 tataccccag attgaagata tattaattat atttcctttt ttttctgata tcatgtattt
    12601 taaagcaact tgtgaacaat aaaagcttcc ttttaaatta atattcatca tattatccca
    12661 atcttcatca gttatatctg taaacaactt atcttgactt acaccagcat tgtttacaag
    12721 tacatccaat cctccaaatt cttttataca ataatctacc atatcctcta catcttctct
    12781 atttgaaata ttagccttaa aaagttttac agaaaaattt ttttcattta atatagtgta
    12841 caactcttta gcttcatttt ctgacttatt aaaatttact aatacattgt aaccttcttt
    12901 tgcaaaagct ttagacatag ctttaccaat ccctctagct cctcctgtaa ttaaaactgt
    12961 tttcttcttc ataaatactt ttctccttat attataacca attcagtatt ttatacaaaa
    13021 caaactgccc tttaaagaaa atattttcta taaaaggaca gctcttattt tattaatatt
    13081 tattctgttt taccaatgtt tgcgccagtt cctgatgaag ttcctccatt atctcctgag
    13141 tttgagcctc ctgaactatc tccattgtct cctgagtttg aacctcctga acttcctcca
    13201 ttgtctcctg agtttgagcc tcctgaactt cctccattat ctcctgagtt tgaacctcct
    13261 gagtttccac cactatttcc tgagtttgaa cctcctgagt tacttccatt attgcctgag
    13321 tttccaccac tatttcctga atttgagcct cctgaatttc caccactgtt tcctgagttt
    13381 gaaccaggtt ctgttggtgt attattctct ggtgttgttg gttttttatc tggtactgtt
    13441 ggagtctgtg tatttgattt ttctgaacct ttgcttacat ataggtttac ctcatcacct
    13501 tcctgagctc ttgatgagcc agcacctgga ctctgactta aaactgtacc ttctttatat
    13561 attgagctgt attcatactt aacagttccc acttttaagt tattttcttc aataagttgt
    13621 tttgcattac tcaaagtaag tcccactaca ttaggaacag atgcctgact tgaaccttta
    13681 cttacgacaa catttatagt atcacccttt ttgacatttg tacttccacc ttcaggtgtc
    13741 tgtgatatta tacaattttc atcaacttca ctattatact cttcactttt tacactcagg
    13801 tataatcctt ctttttcaac agcactttga gcttcttcta atgtcatatt tacaagatta
    13861 ggaacagtca aagatttatt gccaaaccct ccagcaaata aaaacttata tgctaagaat
    13921 acttgagcac acaatatcag tatcaaaaca gccgcaatta cttttaatct tcttctagat
    13981 tttgggcttt cctgtttttt cttctttttc ttagttctct tacttggagt actcttaggt
    14041 ctctgattct ttttagctct cattatctct tcctcttctt catcttcatc atcatcttct
    14101 tcataaaaat catcatagta atcttcatca tcatccaaat cagctacttc aacaggctta
    14161 accacttttt caggagctgg ttttgcaagt gttggattta caactttatt aatttccttt
    14221 tcatcaattt tcttagttgc aaaatcatca tattctttta taaaatctaa atcaatattt
    14281 ttttctatat actctatatc ttctatcaat tcttcagcag tttgatatct atctgcacta
    14341 gatttttctg taagcttttt tattgtagtt cttacacttt gaggaatcct aactttttct
    14401 tctgatgtaa agtctatatc atcattaatg tgttgaagtg ctatagaaat tggactatct
    14461 cccctaaatg gaacttttcc tatcaacatt tcatataaaa ctatacctag agaatacagg
    14521 tcagcattat tagttacaaa tttaccttta gcttgctctg gtgaaaaata atgcactgaa
    14581 cctattatac taccaatatt agtcattgtt gaatttgaaa cagctttggc tataccaaaa
    14641 tcagccactt ttactactct tccttcattt gatatgagaa tattatgagg ttttatatct
    14701 ctatgtataa tccccttttt gtgcgctgca ctaagtgcca tagctatttg ttttgttata
    14761 tctaatgcag tatattcatc taatatacct tcatcttgta taatctcttt tagattcttc
    14821 ccatctacaa actccattac aatgtagtga acttttccat cttcaccaac atcataaaca
    14881 tttactatat ttggatgtga aagacttgct actgcttctg cctctctttt aaactttgtt
    14941 agaaattcat catcatctac aaattcaggt ctaagtactt taagtgcaac agtcctgttt
    15001 aataatctgt ctttagcctc atatacaaag gccattcctc catcaccaat cttcctgatg
    15061 atttcatatc gatttcctaa aattgtatct cccacaatat caccgtccta attatacttt
    15121 tatcactata accgaaacat tatctttacc agaaacatca ttggccatac ttatcaattc
    15181 ttcacttata ttactagttc tctcatattc ataatctaat attaaatctc ttatatcttc
    15241 atcatcaata aacccagtta atccatctgt cgcaagaagt attatatcac tttttttgat
    15301 ttctttctta aaaatatcta caacaaccat atcatctgtt ccaattgctc tagttattct
    15361 attcctctgt ggatgtcttc ttgcctcttc ttctgtaatg acatgggcca tcataagttc
    15421 ttcaaccacg gaatggtcta tagttatttt atcaaatttt tcatctgtta acaaataaca
    15481 tctgctatct ccaacatttg ccacatataa gacattatta tacactatag ccgtaactaa
    15541 tgtagttccc attccttcaa attctatatc ttccatagat tttttgtgca caattgagtt
    15601 tacattatta taagcctgtt ttaatatatc atctatatag tctattttta cattatcatg
    15661 ttgtaataag ttttccttta gaaaatcaat tatattttct actgctaatt tactggcgac
    15721 ttcacctttt ttatgtccac ccattccatc agctatggca aatattccta ttggaccatg
    15781 ttctgtatct ataacttctc cctcacaata gtcttcattg ttttttctta tttttcctat
    15841 atgggaggca caactataaa ccatacgact tcctccttac aaattacttg tattttcttc
    15901 tcaattgccc acaagctcca ctaatatcag aacccataga catcctaact gtggcaggta
    15961 tattgttctt ctccaaacta tccctaaatt tataaataaa tgctttatct ggcttttcat
    16021 attctctttc ttcaacttta tttattggaa ttaaatttac atgacatagc atacctttta
    16081 ggagcttagc taatgccttt gcttcttttt ctgagtcatt tacccctttt ataagagaat
    16141 attcaaatgt aacccttcta tttgtctttt ttatataata tctacatgca ttcaatattt
    16201 cttcaataga ataagcattt gctacaggca taatttttct tcgttcttca tcatatggtg
    16261 aatgaagtga taaagctaaa tttatagcta tttctaagtc tgccaactca tacattttag
    16321 gtactattcc acaagttgaa agagttatat gtctataccc tatattaaga ccattttttt
    16381 cgttaattat ttttaaaaat tgttttgtat tttcaaagtt atcaagtggc tcaccactcc
    16441 ccatcaatac aagatttgat accctcttac cagtatcttc ttgaatttta ataacttggt
    16501 ctaaaatttc ccatggctct aagtttctaa ttaatccatc cattgtagat gcacaaaaat
    16561 tacatcccat cctacaacct acttggttag aaacacagac agtaactcta ctatcataat
    16621 ccatcatcac agtttctatt atatttccat catctaataa aaacaaatat ttttttgtat
    16681 tatctacctt agattctaac tttaattcta tatctatatg ccctatacat gaaacctctt
    16741 ctaatttatt tctcaaactt tttggtatat tgttcatatc atcaaaagtt tttgcaccct
    16801 tatatatcca agagaatatt tgacttcctc taaagggctt ttcatctata gttttcataa
    16861 attctttaag ttcatcttca gtaaaatttt ttaatactat ctttttcttt tccattctct
    16921 cactacctta ttctctttaa ttttgctatg aaaaaaccat ctataccatg aatattagga
    16981 tatatcttta aatatccttt atcttgattg tctaagtcta catttactgt atcaattgga
    17041 acaaattcaa agttgtgatt ttcttcaaca aaagaagtaa ctatatttat attctccaca
    17101 ccttgaattg tacatgtact atatactaaa gttccaccaa ctttgacata tttagatgca
    17161 ttttctaaaa ttttcttttg tatagaagtg atgtccttta actcttcttc tttcttatac
    17221 ttaatttctg gtttacgtct tattatacca aaaccagagc aaggaacatc tgctaaaaca
    17281 taatcaaatt tacttatact attttcatca atctcagaag catcaaatct ttctacaaac
    17341 acatttttaa gccccaatct atttacagta gagtttataa gctttagttt atgctcaaaa
    17401 atatctctag ctactacttg accagtattg ttcattaaag tagctaagtg agtagatttc
    17461 cctccaggag cactacatac atccaaaata aatgaacctt cttttggatt aattactttc
    17521 cctactatca tagaacttat atcttgaatt gtaaataaac cagccttaaa taattcattt
    17581 ttttctatat ttttcatttt ttcgacttta atagcttctt ctaccatagg aacttttaaa
    17641 catacaatac ccatttcact taatttttca ataagctctt ctctacttat tttaagtgta
    17701 ttcgttctta tatagatact aggcttttca ttatttgctt ccaacaaatc ttcagcaaat
    17761 tcttgtccaa atttatctat ccagtttttt ataatccaag agttatagga atactttata
    17821 gataagtatt ttacactatc ttcctcagtt atgtccatta tagtctcttt agctcttatt
    17881 tcatttctaa gaatagcatt tatgaaacca gaagattttt tatcatattt cttagataat
    17941 tttacagttt cattaactgc tgcataatct gatatactat ctaaaaataa aatctgatat
    18001 gttcccattc ttaaaaagat tttaacataa gttgacattt tttttacctt tatttttgaa
    18061 agtttattga ttatataatc taaataatat ttattttcta ttactccata aattaactct
    18121 gttgcaagtc ctctatccat atcacttatt tctaaatttt taaaatgctt atttattgca
    18181 atatttgaat aattgtcatt tttttctata tcacataata ctttaaatcc aacttctctt
    18241 gcatccatat aaatttctcc cttttattaa acataaaaaa gggttgtttc taaacaaccc
    18301 actttacttt tagtcatttc ttctttgagc tattagtaac aatctaagta attgaagtat
    18361 tgaaaccaat gctgctgcaa catatgttaa agctgctgct gaaagtactc ttctactttg
    18421 tcttgactca ctttcatcta taatacctaa attcccaagt tgaacgattg ctctacttga
    18481 agcattaaat tctacaggca atgttattat ttggaataac actgaagccg aaaacaatag
    18541 tataccaatt tttaaaaatg gaccagccat aataaatcct aaaaatatta aaaaccaaga
    18601 tatacttgaa gcaaaattca ctactggtac taaactactt cttatttgaa gaggtgcata
    18661 acctttagca tgttgtatgg catgaccaca ctcatgtgca gctactgcaa cagaagttat
    18721 agatgttcca taatatacat cttctgacaa ccttacagcc tttcttcttg ggtcataatg
    18781 gtcacttaga tgccctctaa ccatttctat ctttacatca tacaaaccat ttgaatcaag
    18841 tactcttctt gctgtttgct ctccagtata tcctcgacgt gtatttactc ttaaatactt
    18901 atttgttgtt gaactcactt taaattgtgc atatatagta agtaatatag ctggaattaa
    18961 tacaaccatt gttgggtcaa atccccaaaa accgccatat acagggtaca taaataacac
    19021 tctcctaaaa tatattttta ttatacatcc tcttaatatt cacttaactt aaaaacagtt
    19081 taattacaaa ctaatatatt ttttcctgtt aaaaagccta tagctttttc aatatccatc
    19141 atgtctacat tagtatttac acaaggtcca ttaggtcttt tgttaaatac acctaaaaca
    19201 ggaatttttt taatatcttg tattccagat gtcaaatctc tttcacatgc aacagctaca
    19261 acagctttag gtttagtgtc cattataatt tttctagcta gggttcctcc tgtagctaca
    19321 aatattttta cattagtttt ttcctttagc ttaattaatt ctcctatgtt gcatcttcca
    19381 cattctttac aattatctat atcattagta acttttaatt tacaactatt ctcttgaata
    19441 caatgaggta ttaaaattat tatatcctct ggattaaaat tatatttatt actgtatata
    19501 tattcattat ttaactttat atatattctt ctaatatcac tttttgacat accaaatgat
    19561 gatgcaatga aagatataat aggaaataat ccactgacaa tattaaaatt tattttcatt
    19621 atatttgagt taacatactt tcctttaatt actctataag taactgctgt tgaacatagt
    19681 attataagta ttagtgatat tattataata tttatcatca aggagaatat ttgagttaaa
    19741 cttattataa aaaaattaac aactcctatc aaaattccta atattaaaat caaaatccca
    19801 acacttgtta aatacttctt tatatctgtc atacttttca cctaacccaa tactaaacca
    19861 gtttctatag tattcccttt tatatattca ccaaccaaca ttctcttttt attcggcatc
    19921 tgaatttctt caattaaaag tacattatct atagtactta cttttatccc atctttgctt
    19981 acatcaatta ttgtacctac atcttttgtg cttttttcat ctaacacttt agttttccaa
    20041 actttcatta tgacatcatt ataggtagta taagcacttg gccatggatt tactcctcta
    20101 actaaattgt gtatttctct ggcacttttt gaaaaatcta tgttacctaa tgatttattc
    20161 attattggcg cataggaaaa ttcttcatga ttttgtactt ctcttggagc atttccttct
    20221 tcaattaatc ttaatgtttc ttttaaagtt tctgctccta tattcatcat tttatcatga
    20281 agttctcctg ctgttatatt ttcatcaaga tttacttctg tctttagtat catatctcca
    20341 gtatctaatc cttcatccat atacatagtt gtaacaccag tcttttcttc accatttatt
    20401 attacccaat ttataggtgc cgcacctcta tattttggaa gtaaagaaac atgaacattt
    20461 atacatccaa gtttaggaat ctctaatatt tcttttggaa gtatctgtcc aaaagctaca
    20521 actactatta catctggatt taaagattta attttatcta taaattcttt atctctagcc
    20581 tttactggtt gatatacagg tatattattt tcaattgcca gttcttttac tgggctcata
    20641 cctagttttt tacctcttcc ttttggttta tctggttgag ttacaacacc caatatttca
    20701 tacttctcat ctattatttt ttgtaaacat ggaactgcta tatcaggagt tcccataaat
    20761 actattttca tcaaatcaac tcctactact tttctatttt gtcaacaaat aaaattccat
    20821 ctaagtggtc tatctcatga caaaatgctc tagctaaaag ctcttctccc tctaactcta
    20881 ttgtttcacc atttctattt aaagctctga cctttacata atttggtctt ctaacatttc
    20941 ccgcttctcc aacaacactt aaacatcctt cctcatctat ttgctcccca gatgtttcta
    21001 ttatttcagg atttataagt tctataagat cttctccaat atctattact actactcttt
    21061 ttaatattcc aacttgaggt gcagccagtc caacaccatc tgcatcatac atagtttctg
    21121 ccatatcatc taataattgt attatctttt catcaatttt ttcaaccttt tttgattttt
    21181 ttcttaatac tggttctcct atttgaacta tttgtcttaa agccattata attcctccta
    21241 aatttataat acactgtttg gattaatatc tatagataca ttgatattct tatcaaatat
    21301 caaatctcgt tttgttatac atatatattt tattataccc tttaagagat taatttcaat
    21361 attttcatct ttaaagagta tttgccatct ataattttga tttatttttg atattgaaca
    21421 tggatttgga cctaatataa atcctaaatc atttataccc cttttttcta ataaataaat
    21481 caatgatgca tgcatatttt ttatattttt tatcactaat ctttcatctt caccactaac
    21541 aactacactt aacatattat taaaaggaga atatccaaat gcttttctta tttttatctc
    21601 atcttcataa aaaccctcat aatcatattc aatagcatgc ttaatagcat agtgttcagt
    21661 gtcataagtc tgaagtacta cttttccctc tttatcagct ctccctgctc ttccagagac
    21721 ttgagttatc aattgaaatg tagtttcaaa acttttaaag tctggaaaat ttaatatcat
    21781 gtctgcagac aaaataccta ctaaagttac attctcaaaa tccagccctt tacttaacat
    21841 ctgtgttcca ataagtacat ctgcttcttt atctttaaat ttatttaata tctcatctaa
    21901 agcacctttt tttgaagttg tatctttatc cattctcaaa gtttttatat ctggaaaaat
    21961 atattttaat tcctcttcaa ttttttgagt tccaactcca aatggcttaa cataagtact
    22021 tttacattca ggacactctt ttggaatttc tttctcatat ccacaatagt ggcatcttcc
    22081 agtattactt ttcttatgat aagttaaaga tatatcacaa ttttcacatt gaaacacata
    22141 tccacacttt ctacaagata caaaatttgc ataacctctt ctgtttaaaa atagtattac
    22201 ttgattattt tcttcaatcg cataacgtat ttctttttgt aatttcaaac taaatatact
    22261 tctatttcct ttatctaact catctttcat gtctaccact tcaatgtttg gaagaggctt
    22321 atcatttgct ctacttttta tatttataag tttgtattct ccagattttg ccctataata
    22381 ctcttcaatt gagggcgttg ctgaacctaa aataagagtt atattatttt ttaatgccat
    22441 aaatctagca acttctatag cattaaattt tggattcttc tcagacttat atgatgactc
    22501 atgacattca tcaattatta ttaagcctaa ggaattaaaa ggtgcaaata aagctgacct
    22561 agctccaatt aacactctta ctttacctgc tttgacagct ttgtaaacat catgtttttc
    22621 tccttcagat agtttactgt ggaacactcc aactaaatct ccaaacctac tctttagcct
    22681 atctattgtt tgaggagtta atgctatctc aggtactaag aatatactgt ctaagccttg
    22741 atttagagca aattctatta tttccatgta aacttcagtt tttccacttc cagttactcc
    22801 atgaattaag tatggttttt tatcatctac aaacatattc gactttatct catcaactgc
    22861 ttcttgttgc tcattgttta attttatact ttttagtacg gatttgtaaa tactttttgg
    22921 ctctctataa taatccttaa attctagggt tattagcttt ttcttactta gagaatttat
    22981 actttgtttt gaagcattta ataaatccaa taaatcattt atctctacat tttcattatt
    23041 tttaagaaaa cttaatatct ctttttgttt acttcccaca tttattttat tttgctctat
    23101 ataatcgtca atcttatcac tttctaaact caaagatata taacatactt tcttttcatt
    23161 tttgtgattt ttatattccc aacataattt tataattcca tttttattca ttgtgtacaa
    23221 agaactattt aagtttggta tatattttaa cttatcaacc ttaattttac ctttactatc
    23281 aatcactttc tctactattt ctctattttt atcacttaaa gttgatattt tttccttcaa
    23341 ttcaatatca tttaatccac ttaaactttc acctaatagg actactttat aattatttaa
    23401 tttgtatccc tttggatata tcaaatttat acattctata taagtacaca aatatctatt
    23461 tttcatccaa taaaccaact ctaaatcttc ttttctaaat attggatttt catctaatat
    23521 atcaataatt tcttttattt ttattttttc atctaagcta tctgtaaatt gaaatacaaa
    23581 ggcttctgtt ggtttattac cttttccaaa tggtactaat attctgtgcc cgatttttat
    23641 tacatcagat aaaaatacag gaatttgata tgtaaataaa ttatctgtat atatagtatt
    23701 acttctaact attacctttg catatttttt cattataaaa cctctactta aatttaatat
    23761 attgttcata ttttaatata ctttacaaga tattaaaatt tcagaattta taaacaattt
    23821 caagttatta atattaactt tattttaata attaactatt aattatattt tatactttat
    23881 gacaaaataa atgctgaaaa ctacaattta actgacattt gcgagttttt actcgctaaa
    23941 attaaattgc agctttcagc cactttagct gtgataaaat acacaaatat catatttaaa
    24001 aaaggtctta tttctcaagc aacgctttaa ctttatctaa aataacattg gctacttctt
    24061 cttttttcat cttaggatac tcagtaatat ttccctctac atctattatt tttacaatat
    24121 tagtatcagc tctaaaacca gcaccttctt tagttaaatc atttgccaca ataaaatcta
    24181 aatttttctt ttttattttt cctttggcat gttctataag gtcattagtt tcagctgcaa
    24241 atccaattag tatcttatct tcttttattt ttcctatttc ataagcaatg tcataatttc
    24301 tacttagttc tattgataaa tcatcattct tctttttaat ttttttgtca gaataagttt
    24361 ttggtttata atctgcaact gctgcacttt gaattactat tttattttca cttaaatttg
    24421 atactactgc ttcatacaaa tcttgtgctg actctatttg aacaaatttt ttaagatttt
    24481 gtggaataga aagatttgta ggacctgata ttaatgttac atcagcacct ctagctatag
    24541 cttctcttgc tatagaatat cccattttac cagaagacct attagttaaa tatcttactg
    24601 ggtctatact ctctacagtt ggaccagctg taactatcat actttgacct tttaaatctt
    24661 gctctttagt aagattagtc aaaacttcct ctactattat atctggatta gctagtttac
    24721 ctgttccaat atctccacat gcaagccttc cactttccgg ttcaataaat ttatatccaa
    24781 agctctttaa agtttgtata tttttttgaa caattggatt ttcatacata ttagtattca
    24841 tagctggtgc tataagaacc tgtgcttttg ttgccataac agtagttgtc aacatatcgt
    24901 cagctatacc atttgcaatc ttgcctataa catttgcact tgctggcact attaagaata
    24961 aatctgcttt ttttgctaat gatatatgtt caacttccca agtttttggt tcttcaaaca
    25021 tatcttctac tacataattt tgactcagcg attgaaatgt aagtggttta acaaattctg
    25081 ttgctgattt tgtcattata acatggatat ttgcatttag tttttttaac ttacttacaa
    25141 catcacaagc cttatatgca gctataccgc cacttatgcc aattacaaca gttttatctt
    25201 ttagcatgac cttactcctc ttctgaaatt tgttggtgtt gctcagcatg atgtctagct
    25261 tcttctatct ctatttcctc ttctgtcaat aatctatatg ttatttttcc acttgcaact
    25321 tctttagttg ctacacatac aggcttttcc ttagttttat ttgaaacata tggttcttct
    25381 ccatcaatta attttcttgc tctctttgaa acagtcccta ctaaatagta tctgttgtct
    25441 attttttcta gtacctcatt gatagatggt tttaacataa ttataattcc tccttaaatt
    25501 tctctattat aatattttta tatctgctaa ctttattctt ttcagatgat attatacctt
    25561 ctatttcttt agcagatgtt tttacatctt cattaaatat aaaataatca taatccttaa
    25621 tcattttaat ttcttcatat gcacagctaa atctcttctc tatttcagct tctgtttctg
    25681 tacctcttcc aacaattcta ttttttaatt cttcaagtga tggtggtagt acaaatataa
    25741 atactccctc tgggcaaact tcttttattt gttttgctcc ttgcatttca atctccagta
    25801 atacatcttg tcctttttct aaacattcca ttattgccgc ttttggagtt ccataaaaat
    25861 tatcataaat ttgagcatac tccaaaaact cacctttttc tatcatctct tcaaactttt
    25921 ccttacttat gaagaaataa tttactccat caacttctcc agttctgggt tttcttgtag
    25981 tagctgatac agaaagtttt attgtatcat tttctttaag tagttctttg caaatagtac
    26041 cttttcctgc acctgatggt cctgatacaa ctagcaataa tccttttttc acaaacattt
    26101 aagcattccc cttctaatat tgtattctta ttctatattt tgtatttgtt ctcttatttt
    26161 ttccaattcg ctttttactt ctactactaa attagttata tttatatcag atgacttcga
    26221 acctatagtg ttagtttctc tattcatctc ttgtattaag aaatctattt ttctacctat
    26281 agagtcgtct ttaaatatag tatttttaag ttgccctata tgacttttaa atctaactat
    26341 ttcttctgtt atactgcttt tatctgcata tattgcaact tcttgtgcta atctattctc
    26401 atctattata cttgggtcat ccagtaattc acttattctc aaattcaatt tttctctata
    26461 atcttctaca acactacttg agtatttttc aatctcttct atatgatttt ttaataaatc
    26521 acatctcatg gcaatatctt cagctaattt tttaccttct tcacttctca tttctcttaa
    26581 tttgattaat gcatcttcaa ctgcttgatt caacattgac cacaataaat cttcatcttc
    26641 ttccttttct tctattttca caatatcagg aaactttgca acattcataa cgctaatatc
    26701 atctataata tcaaatttat tttttatttc ttttagtatg tctatatact gagatgctaa
    26761 tccctcatca aaatttaagt ttacatcttc tttgcctaat aagtcaaact ttatgtataa
    26821 atctactctt cctcttttta cataattttt tactaaattt ctaactttat cctctaaaaa
    26881 tgaaattttt cttggaagac gtatgtttat atctgaatac ttatgattta tcgttttaca
    26941 ctctactaaa aagtaataat tatcatcctt gtattctcct cttccaaaac cagtcatact
    27001 tatagccatt aattaaacct ccaaaatacc ttcacatatt tttactgctg gacctgtcat
    27061 ataagagaaa tctcctacat ctattttaac tgttccacct tgagacgtta catttacact
    27121 actttctacc atattaagta aattgcatac tattgctgat gcagtcatac cagtaccaca
    27181 acctaaagta tatccacaac ctctttccca agtataaaca tgtatattat acttatcttc
    27241 tacctttaca aaatttacat tcgttttttc aggaaatatt ttattatttt caatctcttt
    27301 tccatattta caaacatcat ctacatctaa ttcatctact ataactactg tatgaggaac
    27361 ccccatcaac atagaagtaa ctttaagctc cctatctaaa acctttatac tttcttgcac
    27421 aaaacgttct ttatttgtaa gtacaggaac gtccttagct ataaaacttc cttttcccat
    27481 attgactctt atagagttta tttctctatt ggtaagattt atttttatat cttttatacc
    27541 atctaatgta tctactgtaa aactttcttt tttaactata caattatcat aaacaaattt
    27601 acaaaaacat ctaaggccat ttccacacat agctgctctt gaaccatcag aattataata
    27661 taccatttct acatctgcaa cttttgaatc tttcacaact aacaatccat ccgctcctac
    27721 agaaaatctt ctatggcata ctttttttgc taaagcagta taatcagatg gatttatctg
    27781 gtcgaatctt ccatctattg ctataaaatc atttcctatt ccatgtagtt tccaaaattt
    27841 cataagtatc gctccttcgt aaattaacgc ttaaactatt ataacataaa aaaattccca
    27901 taaacttata atttatggga aaagttttgt aatgacaact ataatttgag gatgtgaggg
    27961 agagttgcca ttacaattag ggtaattata ttatgttttt aagctagttc tttctatgtt
    28021 tatatattaa tatttctata tgaattttat gtatgtttta tttaaactgt ctatgaattt
    28081 tttaattaaa catctggtaa ctagctataa tccttctagt agatatctat taacatacac
    28141 ctattaattc tttaagtgtt tttaaaacat agtctaaatc ttccttagta atatctacat
    28201 gagttacaaa tctatattca ccattttcca ttttattaat ttttatattt ctttcaaaaa
    28261 attcttttat taaaacatcc tcttttataa tatcttcact catcttaaaa aataccatac
    28321 ttatatctgg attaacattg tttacttcaa tgccatcaat tttatttaat tcacaagcta
    28381 aatattttgc attttcatgg tcttcattca atcttaaagt cattttttca atggcaatta
    28441 taccagctgc tgcaactata cctacttgtc tcattccccc acccataact ttttgctttc
    28501 ttctagcttt tgctataaat tctttacttc cagcaagtat agaacccatt ggtgcagcta
    28561 aaccttttga cacacagaac ataacactat cacaacactt tgctatttct ttagcttcta
    28621 cacccaaagt taatgctgcg ttaaataatc ttgctccatc taaatgtact ggtaagttat
    28681 gtttctcagc tattttttta atttctctta aattatcaag actcacaact gttccacctg
    28741 aatatgcatt ttctacacat ataagccctg tctgtggttc atgtatgtca tctcttctaa
    28801 ttgccttttc aacaagttca ggatttagag ctccactttc gtcttcatct atagttctaa
    28861 gctgaactcc agctattaca gcaggtgccc caacctcatg agtcacaatg tgataacctt
    28921 taccaataat tatttcttgc cctcttaggc aatgagtaaa tagagctaat tgattcccaa
    28981 aagttccact tggaaggaaa atagcatctt cttttccaac tttagctgct gctaattctt
    29041 caagtttatt tacagtaggg tcatctctaa agattgaatc tccaacagga gcctttgcca
    29101 tagcttctct catttcgtca gtaggcatag taacagtatc acttcttaaa tcgataaatc
    29161 tcattttttc aaccttcttt catatttata tactatagaa ccttaaaatt gattttttct
    29221 gtaaagttca tatactttca tagcttatac tctacacaat aattatacta tatttacttt
    29281 atatttaatc tactttaaat tattaatttt aaaaataaag tttcctctct aataaatata
    29341 aataattata caatttaaat attaatctct attgtttaaa ttccccctag tcacaaagcg
    29401 tatattatgt gtataattaa tttactgact cattttggtc aaagaaaggg gtgctataaa
    29461 tggcattaga tggattagtt atacattcaa tagtcgatga attatcatca aaacttacag
    29521 gtggaaaaat agataagata catcaacctg aagatgatga ggtaattttt aatataagaa
    29581 ataataaaga aaattttaga ttggttttaa gtgcaagtgc ctcaaatcct agagtttatc
    29641 ttacaagtaa ttatcaaaaa gaaaaccctc taaaagcgcc tatgttttgt atgttactta
    29701 gaaaatacat acaaggtgga aatatagtag aaatttctca aataggtttt gaaagaataa
    29761 taaaaataag cgttgaatct ttagatgaat taaaagaaaa gacagttaaa aatattatga
    29821 ttgaaattat gggaagacat agcaacataa taataactca tggtgaagaa aataaaatta
    29881 tagattctat aaaaagagtc ccttttagca taagtagagt tcgtcaagtt ttacctggtc
    29941 atgattattc attaccacca gaacaaaaca aactcaaccc tcttgatgat atatctaagg
    30001 atttgtttat taaaaaccta gaggaattgg aaggacctat ctttaagtct atatattcta
    30061 gatttctagg tataagccct attatagcta aagaaatttg ctacagagca ggtgtaaatc
    30121 aaaatgctat cataaaggat atatctgatg aacaatttga ttctttacac aaagtatttt
    30181 gtaatttatt caatgatata aattctaaca aatactctcc ttgcattatt atagataaaa
    30241 aggtggataa ggtagttgat ttcagttgta taaaccttac attatttagc gatttatcat
    30301 atataaataa ggatagtatg tctcgtattc tggaagattt ctatagaact aaggatatta
    30361 aagatagaat taatcaacgt tcatctgatt taaagaagag tatatctgtt aagcttgata
    30421 gactttataa taaacttaaa aaacaagaag aagaattatc agaatccgaa aatgcagaca
    30481 tttataagat aaaaggagaa ttgattactt catatattta tatggtagaa aaaggtatgg
    30541 aaagtattga agttgctaac ttctatgatg aaaattgtaa tgatgtaaca atagaattaa
    30601 ataaaaactt gacaccatct gaaaatgctc aaaaatactt taaaaagtat aacaaaatga
    30661 aacatgctaa agtcgagata tctcatcaaa tttccttaaa taaggaagaa atagattact
    30721 tagaaaacat aattttaagt attgaaaact gtgaaaattt agctgaatta caagatataa
    30781 aagaagaact tgctaaagtt ggatacatca agactcaaaa gaaaaacagt aaaaaggata
    30841 ctatcccatc tacaaaacct catgaatttg tgtcttctga tggatttaaa atattggttg
    30901 gtaaaaacaa taagcaaaat gactatctta cactaagact tgctgataat gatgacttat
    30961 ggatgcatac aaagaatatt cctggttctc atgtaataat aaagtgtgct ggtaaggaag
    31021 tccctgataa tactgtattt gaaggagcta tgctagcagc attctttagt aagtcaaaac
    31081 tttcttctca ggtgcctgta gactatacta agagaaaaaa tgtgaaaaaa cctagtggtt
    31141 ctaaaccagg tatggttatt tatgaaacta acagtactat ttatgttact cctgaagaag
    31201 aaacagtagc caagcttaag gttaaatctg aataaattct gcactaaata caaaataaat
    31261 gttagacaac tacacattat cattctaaat aaaaatagtg gccttcttat aaagtacaat
    31321 aatagggcca ctttctaatt agcttacttt taattttcta acaaaatact tttctgtttt
    31381 aataactata cttatattcc caacaaatat tttcagtttt cttatataga tgtaattaaa
    31441 aagttaacac taaaagctat tttttaattt agtgatttgt tttagtaatt cattatattc
    31501 tgattcatac aattcttttt ttagattatc tttttctata gataacatag atataacagt
    31561 tgtaagtcta ttttctaata ctagaagatt ttcttttttg atttggtttt ctctactagg
    31621 agttttttta tttctgctaa ttatatattc gtcataatta caatcaaact cccttatttt
    31681 agcatctttt atctctataa tatagttaca aatatgtgat acaaatactc tatcatgaga
    31741 cactataagc atagttttat cagtatttat cagtgccttt tccagtgctt ccatagattt
    31801 tatatctaag taatttgttg gttcatcaaa tactaaaaaa ttattatctt ccaatattat
    31861 tttacatagt gctattttta ccttttctcc accactaagt acttttactt ttttgtagac
    31921 atcatcacct ttaaacccaa ataggcttaa gtttatcctt ataaaagatt catcaaatga
    31981 actattaact ttagtattgt atagaacact ctttttctca tctaataaac tttgattttg
    32041 gtcaaaataa cctactttta ccttgttatt tatttttata ttgtcatttt tatcagccaa
    32101 tatttctttt atcagagtac tttttccaca cccattatca cctaataatg ctatcttttt
    32161 acctcttttt atcttaaagg aaacattatc taacaggagc ttattttcta actttagagt
    32221 catatctttt atctctacta gatttttgct aattatctcc ataccatctt gaatcttaat
    32281 gttcatttct ttagaaactt taggtttatt ctttacctca agtttatcaa ttctgctttt
    32341 tatagccttc acatttgagt ccaacttttt cttattagtc tgaccaccca ttttgtgcaa
    32401 cctagcttct gaattaccca ttctttttgg tgtttttctt atgccctcac ttaaagcttt
    32461 tttatataaa agagcttttt caagcctttt tttctcttta acatattctt gatactcaaa
    32521 atcagctctt tttacttctt catctttttg cattaaataa tcagtgtaat tgcctttgta
    32581 agcttttagt tttccatctt ctagttctaa tatagttgta cataactcat ccagaaaacg
    32641 tctatcatga gatattaata ataatgctcc ctcatgcctt ttaaacatat cttccaaaac
    32701 cccaatgctt tttttatcta agtttgaagt tggctcatca gctattatga tatttttctt
    32761 attactcagc gcatctgcta tttttaactt aaccttttcc cctcctgata agtgttcttc
    32821 aaatttatct ggagcattta aaagactctt ctccttacta tgaccacatg tttcaatatt
    32881 attttcactc tgacttatat aagaatagct ttctgtaagg taagtatatc cttcatcaat
    32941 ttctatttct cccaatattg cttttaataa tgttgttttt ccttgaccat ttgaaccaac
    33001 tagacctatt ttgtcatttt ctaatatttc aagtttatct atatccaaaa ttaatttatc
    33061 tgcataatat ttctttaaat tctctacttt tactaacaac ataaaaaatc cctccttaac
    33121 acacttaaat ataaggagag attctataaa taggcataac aaataaacgt atagactata
    33181 atctactctc cttaatttaa gcattgcaaa taaagccacc aaaataggct tttcaaaaat
    33241 tacaatactt taaattataa aaaatagatt atattctcat tgtcctagtt accttgccct
    33301 ttctttcatt tatttttaat cattatatca acattaatta caaatgtcta tagaaaatta
    33361 actaatttta gttattcaat atagaactta ttctttaaat ttattcttct tcaactttaa
    33421 agtcctgatt atttaaaatt ctgtttaata caattccaac aattgcagct aaactaagac
    33481 cagttatctt tactgtactt gttataggga ttccaacaga aataccttta ttagataaat
    33541 atgttgttcc aagacctatt aataatataa ctgctatgat taatatattt attttacttt
    33601 ctacacaaga actgtccctt atagttttta atcctacata agttatcatc gaaaatagca
    33661 ttatactaat tccacccata actgaacctg gtatactttg aagaaatcca ccaaacttgc
    33721 ctacacatgc aagcaatata gcaaatattg ctgtacgtct aagtattgaa gggtcatagt
    33781 ttttagttat agcaagtact gctgtatttt ctccataagt tgtattggct ggtcctccta
    33841 aacaccctgc aactatagtc gcaaatccat ccccaagtaa tgttctattt aatccgggat
    33901 tttctataaa gtttttacct actacagcac cattagtagt catatctcct atatgttcca
    33961 taaatacagc taatacaact ggagatataa tcacaattgc tcctaaactg aacttaggaa
    34021 ctgtaaagtt aggtattgca aataaacttg cttctgtaat agtagttata tctactaatc
    34081 ttaatatcaa acagattata tacccagaaa aaactgctat taatattcct aattgtttta
    34141 taaatcctct accaaactta ttggttaaaa ttgctattgc taaagttata aatgctatta
    34201 caaaattggc tgaagccata ctaaaagctg ttggcaataa atttaatcct ataactacta
    34261 tcatagctcc tgtaacttgt gcaggaaaat ataatttaat tttatttaca ccaactactt
    34321 ttactataat cgacattata atataaatta atcctgctac tattatacca ccttgagcat
    34381 atgctaaatc tccaccatac gcctctttag ctgctagtat aactggtata aacgcaaaac
    34441 ttgaacctaa aaaggcaggt acttttcctt ctgtacaaaa atgaaatatc aatgtaccaa
    34501 caccagcaca gaaaattgca actgatggat taaaacctgt caaaatggga actaatactg
    34561 tcgctccaaa catggctaat agatgttgta aagacattat cgtttttttc ataaaaacct
    34621 ccaaaattat tatatataaa taaaccctct gtttaattaa tatcttttaa ttaattatca
    34681 tt
//
LOCUS       DAKBNN010000016        32644 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000016 DAKBNN010000000
VERSION     DAKBNN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.016"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            255..833
                     /locus_tag="OUB02_000787"
     CDS             255..833
                     /locus_tag="OUB02_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HCT8884580.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            872..1450
                     /locus_tag="OUB02_000788"
     CDS             872..1450
                     /locus_tag="OUB02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HCT8884581.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1536..2165
                     /locus_tag="OUB02_000789"
     CDS             1536..2165
                     /locus_tag="OUB02_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HCT8884582.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2155..4914
                     /locus_tag="OUB02_000790"
     CDS             2155..4914
                     /locus_tag="OUB02_000790"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HCT8884583.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGRDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            5001..6563
                     /locus_tag="OUB02_000791"
     CDS             5001..6563
                     /locus_tag="OUB02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HCT8884584.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6564..7700
                     /locus_tag="OUB02_000792"
     CDS             6564..7700
                     /locus_tag="OUB02_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCT8884585.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7764..8840
                     /locus_tag="OUB02_000793"
     CDS             7764..8840
                     /locus_tag="OUB02_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HCT8884586.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFADALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8818..10056
                     /locus_tag="OUB02_000794"
     CDS             8818..10056
                     /locus_tag="OUB02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HCT8884587.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENKYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10110..11198
                     /locus_tag="OUB02_000795"
     CDS             10110..11198
                     /locus_tag="OUB02_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HCT8884588.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIKTIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11226..12017
                     /locus_tag="OUB02_000796"
     CDS             11226..12017
                     /locus_tag="OUB02_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCT8884589.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12132..12686)
                     /locus_tag="OUB02_000797"
     CDS             complement(12132..12686)
                     /locus_tag="OUB02_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCT8884590.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12882..14084
                     /locus_tag="OUB02_000798"
     CDS             12882..14084
                     /locus_tag="OUB02_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HCT8884591.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14160..15140
                     /locus_tag="OUB02_000799"
     CDS             14160..15140
                     /locus_tag="OUB02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HCT8884592.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKYILK"
     gene            15388..16341
                     /locus_tag="OUB02_000800"
     CDS             15388..16341
                     /locus_tag="OUB02_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCT8884593.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRANIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16331..17287
                     /locus_tag="OUB02_000801"
     CDS             16331..17287
                     /locus_tag="OUB02_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCT8884594.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17284..18039
                     /locus_tag="OUB02_000802"
     CDS             17284..18039
                     /locus_tag="OUB02_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8884595.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18131..19096
                     /locus_tag="OUB02_000803"
     CDS             18131..19096
                     /locus_tag="OUB02_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8884596.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     gene            19574..21433
                     /locus_tag="OUB02_000804"
     CDS             19574..21433
                     /locus_tag="OUB02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8884597.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(21725..22324)
                     /locus_tag="OUB02_000805"
     CDS             complement(21725..22324)
                     /locus_tag="OUB02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HCT8884598.1"
                     /translation="MAIELKKGQKINLTKKENSNLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(22427..23224)
                     /locus_tag="OUB02_000806"
     CDS             complement(22427..23224)
                     /locus_tag="OUB02_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8884599.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(23337..23442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(23534..24520)
                     /locus_tag="OUB02_000807"
     CDS             complement(23534..24520)
                     /locus_tag="OUB02_000807"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCT8884600.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            25004..26734
                     /locus_tag="OUB02_000808"
     CDS             25004..26734
                     /locus_tag="OUB02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCT8884601.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            26844..26924
                     /locus_tag="OUB02_000809"
     tRNA            26844..26924
                     /locus_tag="OUB02_000809"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26878..26880,aa:Leu,seq:tag)
     gene            27259..27774
                     /locus_tag="OUB02_000810"
     CDS             27259..27774
                     /locus_tag="OUB02_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HCT8884602.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YKNYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      28266..28370
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28580..31054
                     /gene="gcvPA"
                     /locus_tag="OUB02_000811"
     CDS             28580..31054
                     /gene="gcvPA"
                     /locus_tag="OUB02_000811"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCT8884603.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            31054..32511
                     /gene="gcvPB"
                     /locus_tag="OUB02_000812"
     CDS             31054..32511
                     /gene="gcvPB"
                     /locus_tag="OUB02_000812"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCT8884604.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT        12681 a         3531 c         5616 g        10816 t
ORIGIN      
        1 gtttcaaaat aaaatatgta cttgtttata taaatgatat ataattacat attaaacata
       61 gcttttatac atttaataaa gtatgtaaaa tctatgtaaa aagtaagaaa tataaacatt
      121 tttttaatta tattttaaaa atatcaagca agttttcttc acaaatgaat aaaaaatgtt
      181 ataatatgat taaagaaaaa gataaataat tagcaagatt tgtttagcta aaattaacaa
      241 gggagtgata aattatgggt ataacattat caaaaggcca aaaggttgat ttaactaaag
      301 gaaatccagg tcttaaaaat attttagtag gtttaggatg ggatactaac aagtatgatg
      361 gtggatttga ctttgattta gatacagcag catttttaac aggagcaagt ggaagtgtta
      421 ctaatgatgg agattttgta ttttacaaca atttaaaaca tacatctgga gcagttgagc
      481 atctaggaga taacagaact ggcgaaggtg atggagatga tgaacaaata gttgttgatt
      541 tatctaagat tcctggagaa ataagcaaga tttcttttac tgttacaatt catgatgcaa
      601 cagaaagaag acaaaatttt ggacaagtaa gtaattctta tataagaata gtagataaag
      661 acacaaatga agagttaata aagtatgaat taggagaaga ttttagtata gaaacagcta
      721 tagtagtagc tgaaatatat aagcacaatg gagaatggaa gtttaatgca ctaggttctg
      781 gctttgaagg tggtcttgct gcattatgtg gaaactttgg aataaactta taatttcaac
      841 tataacaata aactaaaggg ggatattttc catggctata gtattaaaaa aaggacaaaa
      901 aatcgattta actaagggaa atccaggtct taaaaatata aaacttggac taggatggga
      961 tacaaattca tttgatagtg gctatgatta tgatttagat gtaagtgttt tcatggttgg
     1021 agaatctcaa agagttgaaa aagatgaaga ttttatattc tacaataact taaaacatcc
     1081 atctggagca gttgaacact taggtgataa tagaactggt gaaggtgatg gagatgatga
     1141 ggaaatatta gtgaatttag agacaatgcc aaagcatata caaaagatag ctgtaacagt
     1201 tacaatatat gaggctgctg aaagaagaca aaattttgga caagtaagca actcatatat
     1261 aagagtatta aattctgaaa atgatgaaga aatattaaga tatgacttag gtgaagaatt
     1321 tagtatagaa actgcaatta cagtatgtga gatatataag tataatggag aatggaagtt
     1381 tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca ctttgtagaa actatggctt
     1441 aaatgtttaa tttaagcaat ataatatatt aaatataaat aacctataca aataatgtat
     1501 aggttatatt atataaaatt ttgtggaggt ttagtatgtc gattaattta tcaaaaggtg
     1561 ataaaataga tttaaaaaaa tctaacccag gactgagtaa catacttgta ggtctaggat
     1621 gggaccctgt gcaacaatct ggaggaggtt tttttaaatc gttatttggt ggaggacaag
     1681 ctgatataga ttgtgatgca tctgtattta tgcttaatca agaaggtaaa ttaagtggaa
     1741 taaaagattt aatttatttt ggaaatctaa aaagtgcctg taaatcagtt ttgcatacag
     1801 gagacaatct aactggtgag ggtacaggtg atgatgaaca aatcctagtt aatttggata
     1861 aagttccatc aaatattcat aaattattgt ttgtagtaaa tatatacaat tgtgtagata
     1921 gaaaacaaca ctttggaatg atagaaaatg cctatataag agttgaagac caaggtaata
     1981 agaaagagat agcaaaatac aatctatctg ataattattc tgaaaaaact actcttatag
     2041 taggagctat atatagaaaa gatggaagtt ggcaatttaa agcaattggt gaaggaacaa
     2101 aagatgcggg tcttaaagaa gtaatgcaaa atttagacag gattgagtgt gcatatggaa
     2161 tttaataatg atatcaaata ttataatgta gataaagaag aagttacaaa agacttatct
     2221 gttaatcctg aaaaaggttt atcagaatca gaagtcaaga ctagaagaga aaaatatggt
     2281 ttgaatgaat ttactccaaa agaagagggt agtttttggg atgatttaaa ggaaagttta
     2341 agtgaaccaa tgatagttat cttgattatt gcagcagtag taagtgcagt aattggggaa
     2401 actcatgatg caatagggat tgtaggtgct atagcaatag gtatagcaat aggtatgatt
     2461 acagaaggtc gttctaagaa agcagcagag gctctttcta agttgactga aaacatagaa
     2521 gtaaaagtat tgagagatgg ggaagtacat caaatttcta agagtgaatt ggtaccaggt
     2581 gatatagtat atattgaaac tggtgacatg gtacctgctg atggtagact tatagaaagt
     2641 ataaacctta aaattagaga agatatgtta acaggagaat ctgatgacgt atctaaaaag
     2701 tgtgatgtag tagtttctat ggaagagata gaatctaaag gagcagtagt agtacaggag
     2761 ccaatacctg caaaacagat aaatatggtg tttggtggta cacttgtagc ttatggtaga
     2821 ggagctctag ttgtaacttc aattggagat aatagtgaaa tgggtaaaat tgctcaaaac
     2881 ttatcagaaa cagatgagca aactccactt cagataaaac ttggaaattt aggtggtttg
     2941 atagccaaag tttctagtgc agtagcagga ttactattta tatttatggt tttccaaatg
     3001 ataagtaaga atgtattaaa tgtagatatg tctggaatat taccgttttt agaatcaata
     3061 gacccagtta aaacagcatt tacagtatgt gttgcgttga tagttgcagc agtgccagaa
     3121 ggtcttccaa ctatgataaa tatgacactt gcaataacaa tgcaaaaaat ggctaaaata
     3181 aatgcattag ttaccaaaaa agaagcttgt gaaacgatag gttctgtttc tgtaatatgt
     3241 tcagataaaa ctggtacatt aactgaaaat agaatgactg tagaagttgc atatgttgat
     3301 ggcagatata ttgaaagttc agaagaggaa ataaatagtt attttgaaga aaactgtatg
     3361 ataaactcta cagctgatgt agagcacaat gatggtgata ttaagtattt aggaagtgct
     3421 actgaatgtg cattgctttt atattacaaa aacgtagatt atagacaagt tagaaaaaat
     3481 tctaatatag tagctcaaaa tccttttact tcagattcaa agagaatgac ttctgttata
     3541 ggtcgagata atcatcatgt gctattatca aaaggagcac cagaagtatt acttgaatta
     3601 tgttcacata ttcaaagagg aaaagacata gttccattaa ctgaaaatat aaaacatgag
     3661 attttagaag aaataaagaa attgcaaata aaatcaatga gaactttagg gtttgcatat
     3721 aaagaaatct cacaagctga agaagaagca gcagtaactg ctgaaagtga tgctatgaat
     3781 gtaagtgcaa tggaaaacaa tttagtattt agtggttttg ttggtataag agacccactt
     3841 agaaaagatg ttatagagtc tgtaaataca gcaaataaag ctggtgtttc tgtaaaaatg
     3901 cttacaggag ataatataaa tacagcaaga gctataggtg aagagttagg cctattaaaa
     3961 aataatatga gagtggttga agcttcatat atagatactt tagatgatga agaattaaaa
     4021 caagagatac aaagtatttc aatcgtggca agaagtaaac cagatagtaa aatgagaata
     4081 gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa caggtgatgg tataaatgat
     4141 gctccagcac tttcaaaagc agatgttggt atagcaatgg gtatatctgg tacagaggta
     4201 tctaaaaacg cagcagacat aattttaact gatgatagtt ttagtaccat tgtaaaaggt
     4261 attaaatggg gtagaggaat ttatgataac ttccaaagat tcgttcaatt ccaattaact
     4321 gtaaatgtaa ttgcattttt agtggctata atatctcaag ttatgggaca agagatgcca
     4381 tttactacaa tacaattatt atgggtaaac atcattatgg atggaccacc agcactagca
     4441 ttaggtttag agccagttag ggaccatgtg ttaaacagaa aacctgttga tagaaaagcc
     4501 aatataattt ctaagtcaat ggtgtataca atagtattaa atgcatttta tataacagca
     4561 atactaatgt tacaatctac atttaatttc ttaggagttg atgaagcgca taagggaaca
     4621 gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg catttaactg tagagagttt
     4681 aatgcagata gtatcttccc taattttaca cataacaaat tagcactaca aataatagtg
     4741 ttgacaggaa ttgcacaagt aatatttact cagctattcc aagatttctt taactctgta
     4801 gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag cagcaagtgt aattgtggtt
     4861 aatgaagttg taaaatgtat aataagactg gtaaaaccaa aaaagactgc ttaaaacaaa
     4921 ttttttagaa gaaaacagac tgactcaaaa tatgaatttt gggtcagttc tgaataaaca
     4981 tttaaattgg gaggatagtt atgggtgact caaaggtatt tgaaaaaata ttctccagcc
     5041 aatcagatag aggaaattac actccttcaa agggatatct aagttatttt atttcgtata
     5101 ttggtttaga agatgaagtt ttgtacaatc tcgagatatt taaaacaaaa caaaatattg
     5161 atagcaaaaa agatattgct ttatttactg atgttatagc aaatcctagt gattttgata
     5221 ttataaatta ttttaaaagt ggacttcaaa aatatagaac tagcatggaa gatgtagata
     5281 taaatatttt gggatttgaa gagattgatt ataaaataaa gcaagcaatg gatagagtgt
     5341 taaaagaaga ggaaaaagaa tttactaatg atagagttaa acaaaatttt atagtgaaaa
     5401 taatggcatg gataaaaatt tatatcggtg ctttagatat aaataaaaat gaagctccaa
     5461 aagtaatatt ttatggagat ataaaaaaac atgaagttta tttactctta atattatact
     5521 tggcaggttt tgatgtttta tatcttaatc caaattcaaa gagtaatata gatattttaa
     5581 aatcagaaag atacaatata gagtttgaag aagccaacat aatagaagaa aaaatttctt
     5641 ttgaagaaag ggttatttta ggggaaaaaa tagataaaag ttctgtaaaa aaagctttta
     5701 ctgttggggc agaagcatca aagcgtataa gtgaggaatt gttaaatgat gcaggattta
     5761 taaaaccttg gcaactccaa gatagaaaaa taaaaaatct acttctaagt tcaactgttg
     5821 atgaaatatc aatatactgg aatcaaccac ttaaattaag accaggattt aagtttaatg
     5881 atgctattgt agaagctcca aattttcttt ctaagattaa tggaatatat aacgataaga
     5941 atgaatatat taagttttta gatttattaa gagattcgga aagttcaaca tttattgaat
     6001 ttaatggaga tgtagataga ttttcaaaag cttttacaag agaagctttt agtttatcct
     6061 ttttattgga ttcaaaaggt gcaatagata aaaactctgt tttaaataat aaagattatt
     6121 ccatatcaac actagcattg aatcaacaaa ttatgatttt agagaaagtg gaagagttgt
     6181 tagaaggaag tatgtttttg aatggtttaa gtggagaaga caagataaaa ggtctattta
     6241 cagtacttca catggataag aaatttgttc atatgatgaa caatttcgat tactcactta
     6301 taaacccaaa attgattata tacatgtaca agagtattgt atttgataaa gaaatagttt
     6361 ttcttatgct tttacttagt aagattggat ttgatattat catattatgt ccaggaggag
     6421 aaaacaatat agagaatgtt ataaataacc aattgattga tgtacacaga ttggataaaa
     6481 tggtatatga ccttaaatta aattcgcttg aaaatgatat acctttactt aaaaaaattt
     6541 ttggaaagag gagacgattt tagatgggga taaatataaa tagtttttca aaagataaga
     6601 actctgtaga ggttactgag aatttgccta cagcagttca agaagaaaac ttcgatataa
     6661 tggaatatac aaataataaa aaaaatgaac tgagaaagtc taaggaagtt gaggcattga
     6721 catctcttat agaagtagaa aatccagata cgattttaca gtttggtagg aaagcatctg
     6781 aaggagtagc aagagtttct gatagccttt taaatacaat aaaattaaat agaaatgaag
     6841 aaaattctaa aatgctggtt catttaacta aaataatgga taagtttgat ttagacgatt
     6901 ttcaagaaac taaggaacca aatttcgttc aaaaactttt caaaaaagct aataatgcca
     6961 ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga agttgagaaa attcaaattg
     7021 agcttgagca atacgaaaga gatatagcac tttctaataa gcaaataggt gctatgttaa
     7081 atgaaaactt tgagttttat aatgaacttc aaaaatatat tgttgctgga gaaatggcaa
     7141 tagaagaaat ggataatgaa atactacctt tctttaaaca aaaatcagag actagtggag
     7201 atcaaatgga tgttgtcaat tatcaagaac tattaaaagt ttatgatatg ttaaatcaaa
     7261 gagtctatga tttaaggata gcagaaaata tagctatcca aacaatacct atgcttagag
     7321 gtatgcaaca taataattat ggtttaatta gaaaaataaa tagtgcattt gtcgtaactt
     7381 taccagtatt taaacaatgt ttatctcaag ctatattact taaaaagcaa gaattacaag
     7441 caaaatctct aaaagcactt gatgacaaaa ctaatgaatt attgcttaga aatgcacaaa
     7501 atgtaagtac tcaaagtgca caaatagcta gaatggctgg aacaagttct gttcaaatag
     7561 aaacattgga aaaaatgtac aatactatca aatctggtat agatgaaact atgagaatag
     7621 aagaaaacaa tagagcttta attaaggata atacaaagag attggaagaa ttaaatacga
     7681 ctataattta taataagtag tattatatta agggctaatt tgtataaagt tggccctttc
     7741 tatgtatatg aggaggctag acattgaaat atttcgatta tatctgcaaa gatgatttag
     7801 agagaatttt tttgaaggag ccagaagatt ttagtgctaa gactgaaaaa gatgtgttga
     7861 aatatgcact gggcgctttt ttatacgttc cagctactca atataatatg atttataaga
     7921 gtattatagg ggatgttaag ggagttagac cattagctat ttgtttagaa gatgctgttg
     7981 gagtaaatgg agagttagag gctatagaga atcttagatt aattttaaaa aatattagca
     8041 atgaaagtat aactaataaa gatggaatac cactaatttt tgtaagaata aaagatgtgg
     8101 agcaactgtt gagaattaaa gagattataa ttaaaaatag tcattttata acaggaatat
     8161 taattcctaa agcaaattcg gagttaattg aaaattgtat agaagcttta gattctatga
     8221 atcttcaaga tatgtatgtt attccgatag tagagacaaa agaatttata tataatgaaa
     8281 aaaaagaact tagttttact aatttgtaca atgctatttt aagacataag tcaagaatat
     8341 taagcattag agtaggcata actgatatat tagggatgta tggtataagg agagataaaa
     8401 acttctctat atataataat ttgatttgtt cttcatttat attagatata atcacatatc
     8461 ttcaaagacc agagttagat atacctataa gtggtggagt atcagaattt tttgatatga
     8521 cgaataagga tataagaaat aagtatatag aagaaatatt attggataaa tatcatggat
     8581 taattggaaa gaccgtaatt catccaatgc aaattcaaat tgtacaagcg ctatctacag
     8641 tatcatatga agattttgct gatgctttgg atatactgga tagtacagac agtaagtatg
     8701 gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac aaatccacat tttttatggg
     8761 ctaaaaaaac attgattcta tctaaaatat atggtgtatt aaataaagga gtagactatg
     8821 aagaattgct taaattttaa ttgtgagatt gacattattg ataatccatt gaatttagaa
     8881 ctaaatgatt ttttagacgt aggaataaga aataatagca aaagacgttt tttgtttatc
     8941 tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg agatggataa tttgggaaaa
     9001 ttaatggcta caatttataa tgaaaaaaat aaggaagaat caaatggtac tgttatagct
     9061 tttgctgaaa ctggtacagc tgttgggcat agtttttttg attacttatg tggagattat
     9121 gaatttattc atactactag agagcagttt gacgagttag aaaatctgga atttttagag
     9181 gaacattccc atgcaacaga ccagaattta tactttggta tgcttaacaa ctttaaattt
     9241 ggagatgaga taatattggt tgatgatgag attaccacag ccaatacatg catgaatata
     9301 attaggaaaa ttcaaagcat ttatcctaaa aagaaataca ctatatgctc aatactcaat
     9361 tgggtaagtg atgaaaattt aagtaaagtt actcgtttag aaagagagct agattgtaag
     9421 atagattttg tatacttatt taaaggaaac tttaaatttt atatagatga agatattatt
     9481 tcaaattaca acgataatta tagtattact aaattagaga ctatttcaag tgataaagta
     9541 aatttagtag taaatcatat ttatgttgat ttagaagatt atattggaaa taagaaatat
     9601 gtaaaatata caggaagatt tggaattaat agaagagaac aatttagatt aaaagaaatc
     9661 gttaagagag aaagtaaaaa aataactatg gccagtaaag aatctttaaa aaatgaaaaa
     9721 gaaaagattc tatttttagg tactgaagaa tttatgtata tacctatgct gtttgcaaaa
     9781 gaaaaagaaa ataaatatga tatatactat cattcaacaa caagaagtcc aattattaat
     9841 attgataaga ataattatcc tattaaaagt aaatttatgc taaagagttt ttataatata
     9901 gatattcaaa attatattta caatatagat aaacataatt tttctaagtg ttatttattt
     9961 gtagagctga ataaaagtga aaattcttat ctagaattta ttgatataat taaaaaaaca
    10021 agtataaaag aattaactat agtatgttgt agctaaagtg ttttaacagg ataaaatata
    10081 tcacgatttt aagataggag ttgagtcata tgagagattt tagcacgtat tctaaagatg
    10141 atgttatttt tttattaaaa gatatatcaa atttaattga agaagaagga aataaagaaa
    10201 gagaattaaa aatacaaaat ggtagacatt attcagaaat gataccaata gagtatgaag
    10261 ttagtgaaga ttatctgaat ttatatcatg agaaactaag agaaaataaa gataaacttg
    10321 cattttctat aggagttatg tgtgaaaaaa taattaaaaa aaatggaaat gatgtagtac
    10381 tagtttcact tgctagagca ggaacaccta ttggtatttt ggcaaaaaga tatataagaa
    10441 gcaagtataa tataagttta cctcactata caatctctat aataagagat aagggaatag
    10501 atatgaatgc aattgaatat atagtaaaga atcatccgag ctcaaaaatt caatttttag
    10561 atggatggac tggtaaggga actatatcaa aagaacttga aaaagcatgt gatgaactta
    10621 atgttaaatt taaaaaatat tttgactcaa cacttgctgt attagctgac cctgctggat
    10681 attcaggact ttatggaatt agagaagatt tcttaatacc aagtgcatgt ttaaattcaa
    10741 ccgtttctgg tttagtaagt agaactgtac taagagagga tttaattggt aaaaatgact
    10801 ttcatggagc taagttttat agacatttaa aagataaaga tgaatctatg aactatatca
    10861 aaactataga agaatgtttt aaaaatcaat ttaagaatat atctgatgaa gttgaaaact
    10921 gggaaagtga cattataaca agagatggat actttgatgt tttaaatata aaagaaaagt
    10981 ataacataac agatattaac tttatcaaac caggtgtagg ggaaacgaca agggttttac
    11041 ttagaagagt tccgtataag attcttgtta aagacttaaa tgataaatct ttagaccata
    11101 tattcatatt agctaaggag aaaaatgttg aagttgaaca aatggattta aaagcctata
    11161 aatgttgtgg aataataaaa aatatgaaag atatttagga aatatattag acaggagaat
    11221 attgaatgat tgttttttca gatttagaca ggagtatcat ttactccaat aaatttttaa
    11281 atgctgatag taaatatact aatatagaaa tttataggga aaaagaaata tcttatatat
    11341 ctttagatac tataaattta ataaagcaaa tacaatatta tggtatgttt atacctacaa
    11401 ctactagaac agtggagcaa tttaagcgta tagaatttaa taaatatggg atttattttc
    11461 cttggagtat aacttcaaat ggaggagtta tacttaaaga taatgaaata ttgaaatcat
    11521 ggagtgaaaa aatagataaa ctaaaatctg attatgagcc cattgaaagt atgattcata
    11581 aatttaaaga ctatttgaat gtagatggaa taactaattt taaagttgca gaagatacat
    11641 ttttttatat agttgttgat ttatcaagat ttaatttgga ttcaatcaaa gaatatacaa
    11701 atatattgga atcaaaaaat tggaagtttt atgtgagtgg cagaaaaata tattttatac
    11761 caaaagaaat ttcaaaagaa aatgctataa aatacttgac taaagaatta ggaatagaat
    11821 atttttatgc agttggagat tctattatgg actatggtat gcttaatatc tcaaataaat
    11881 cctatgtatt gaagcatggt gatataaata aaaatgaaat agaaaattca tttatatcaa
    11941 gttctttcaa tggaatgagt ggaactgaag agatactatc taacatttta gacgagaact
    12001 gcctacttaa catataatga ctgatatata aataataaac tagaaaaatc tgaaataaat
    12061 tatagtaaag tttatcatac taatttattt cagattttta tcaatttata tttaagtaag
    12121 aactcaatat gttaaaatga aggtggagtc attgcccaga ctgagataca tacctcatca
    12181 tcataatttt catacacatg tggtatagta ctgttaaaat aaaaactatc tccttcctcc
    12241 aatagatatt ccttatttcc aagtgttatc ttcattttac cttttattat atagccacat
    12301 tcttctcctt cgtgtgtcac aaattcactg tttttttgtg tatgaccatc tagcgtaaca
    12361 agtataaatt ctattttttt cccagataaa ttaggaacaa gaagttcata aaattttgta
    12421 gaatcattag ttactatttt ttttcgatta tttttcttta ctactatatc agagtcttct
    12481 cttccaatct cttcaaaaaa ataacctata tttatatcaa gagcttttga tattttccac
    12541 attgctacaa tagatggaac aaccatatta cgctcaattt gacttattaa gcctgtactt
    12601 aaatctgaat tttttgctaa agtagagata cttatatttt tagattttct aagagatcta
    12661 attttgcttc caacatctat attcattaga tacaaacccc ctcagacatg attataacat
    12721 ataatttata aagattaata aaataaaaaa aaagttttac aatacaagta ttttttatgc
    12781 tatattgaat tttctgaaaa gatgcattat aatagtatta atattcaata tattgaatat
    12841 tgataacgat attttaattt aatgaaatgg aggtaattaa gatgaagagt tttgtaagtc
    12901 ctgataatat ttgggtatta tgggcagttg taactggatg ggcagcattt agtatttatt
    12961 tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc aataatagct cttgtaggag
    13021 caatgttatt atcaaatcta aatattatac cagtagagtc tgttgtatat gaccaagtat
    13081 ggggatatgt tgttccttta gccatagctt tattgttata tcaatgtaac attaaaaaga
    13141 tatggaaaga aagtggaaga ttgctcatta tatttcttgt aggttctgta ggaactgtac
    13201 ttggagctat gattggattt ttggcattaa aaaatgtagt accagattta aatattgttg
    13261 cagctatgat gacaggttct tatataggtg gaaatgtaaa ctttgctgct atgtcaggcg
    13321 cgtttgatgc gccaggagaa ttagtatcag caacagttgt agcagataat cttttaatgg
    13381 cattatattt ctttgtctta atagctatac catcaattgg attttttaga aaacatttta
    13441 aacatccaca tgtagatgaa atggaaagta taggtataac tgaaggagaa acagtagcag
    13501 caaattattg gggaagaaaa gaaatatctt taaaagatat agcaatagcc attggtagtg
    13561 cttttataat tgtcgcagta tctgtagaac tatcaacttg gtttaaagct ataataccat
    13621 tatcaaatcc ttttttagca atgttaaata ctttatttgg aaatcaatat ctaataataa
    13681 ctactttgac tatgttgttt gccacattta tgcctaattt ctttggtgat ataaaaggag
    13741 cacaggaatt gggtacattt ttaatatata tattctttgt tgtagtaggt gttcctgctt
    13801 caataacatt aataattcaa aaatcaccat tactactttt atactgcgga ataatggtac
    13861 taataaacat gctagttaca tttatagttg caaaaatatt taagtttagt ttagaagaag
    13921 ctattcttgc atctaatgca aatattggtg gtcctacaac agcagcagct atggcaatat
    13981 caaaaggatg gtctaagcta gtagggccaa tacttatagt tggaacattt ggatacatag
    14041 tcggtaatta ttttggtctt ttagtaggaa atattttgat ataattttac gtgaaaaaaa
    14101 gttgtttttt agaaagattt ttatttatta gaaagatttt tatttaattg gaggttgtta
    14161 tgattttcga tggacattca gatacttggg cagatgtaac aataagaagg cttaacgggg
    14221 aaaaagatgt atttagaaga atccatcttg ataattttaa aagagcaaat atagaaggag
    14281 ctatttttgt aatatggata gacccaccat atgataaaga gcctgaaaaa agagttaaac
    14341 aaattataag ctctataaaa gatgaaattg aagatatgaa agatgttgta aacatagtaa
    14401 aaaaatattc agatatagat aaaggtataa gtgaaaacaa aataaatatt ttaattggtc
    14461 ttgaaggatt aagccatatt ggaagaaata tagatttact agattatttt tataaagaag
    14521 ttggtgcaag acatgcatca ttaacttgga atgaacagaa tgaacttgct acaggatgtt
    14581 taggggatga aaatcgtggt ttaactgaaa taggtaaaaa agcagtcaat aaaatggaaa
    14641 aattgggcat gatagtagat gtgtcacatt taaatgaaaa atcattttgg gatttaatgt
    14701 ctgttgcaag taagccagtt atagcttctc attctaatgc taggagttta tgtgatgcta
    14761 aaagaaatct tacagataat caattaaaag aaatttcaaa tacaggtgga ttagttggaa
    14821 taaatagttt tagaggattt actaatagta atcctaagaa gcaagattta atacatttgg
    14881 ttgaccatat agactatatg gtaaatctta taggtataga acatgtatct tttggttttg
    14941 attttagcga ttacttaggg aaagagacta taggttcttt tactggaaat gctagtgtgc
    15001 caagtgtaga tggtcttgaa agcacacata cagcacagaa catagtagta gagcttgaaa
    15061 atagaggtta taaaaagaaa gatatagagt taatctctag tggtaatttt tataggctct
    15121 ttaaatatat actaaaataa tataataaag aacttaaatt tataaaatag tatatttata
    15181 caaataatat ctacatattt aaattggcgt tcataattta ttaaattaaa ttttaatgtt
    15241 actagaagtt tcaattctag taacattttt tatgttaaaa acagatatag atatgtattg
    15301 acaatgataa ttattattga taaactataa aagtgaaaat gataataatt ttcattttaa
    15361 ttaataaaag atttttaagg agggagaatg aagaaaaggt atttagtcat aggaataata
    15421 attttgtctt atttttcttt atttatagga gctgaagata taaatataat gcatatattt
    15481 gccaaaaatc agcataaact tatgatattt atcatgagta gaataccaag attaataagt
    15541 atattgattg ctggtgtagg aatgagcata gcaggtctta taatgcaaca gataagtaag
    15601 aataaatttg tatcacctac aacaggtgct acaatagatg cagcacaatt tgggatagta
    15661 atatgtatgc ttttagtacc aactgcctct atttttacaa agacaataat agcatttgta
    15721 ttttctcttg tagggacttt tacgtttatg aaaataatag ggaagttaca atttaaaaat
    15781 ataatctttg taccattagt aggtataatg tttggaaata taataggttc tatgacggat
    15841 tttatcgcgt ataaatacga tttgagtcaa aatgttagtt cttggatgca aggtgacttt
    15901 tctatgatac ttaaaggaaa ttatgagata ttatatatca caatacccct tataatatta
    15961 gcatatattt atgcaaataa atttactgtt gttggtatgg gtatggattt tgcgacaaac
    16021 ttaggtctta gttacaaaag aattgtcaat ataggtctga taatagtagc acttgttaca
    16081 gtatgtgtag tcgttacagc tggtaatatc ccttttatag gtctaatagt tccaaatatc
    16141 gtatccttgt atatgggtga taatataaga gcaaacatat ggtatactgg tttacttgga
    16201 gcgatatttg tacttatatg tgatattttt ggaagaatta ttatttatcc atatgaaata
    16261 tctatagggc ttacagttgg agttatagga agtatactat tcctttattt aatattaagg
    16321 aggaatgtaa atgaagctta gtctcaatca aaaaatttac tttgtaagta tattactatt
    16381 catatcaata gcacttttta taatttatgg aatagacatg aatcatttag aatatgcatt
    16441 atcacaaaga ataccaaaaa tatttgctat ggtattaggt ggaggatgta tagcatttac
    16501 tactgtagtg tttcaaacaa ttacaaacaa tcaaatatta acaccaagtg tactaggttt
    16561 agattcattg tatgtaatga ttcaaactat aatagtattc atatttggtt caagcagtag
    16621 tctgattata aatgaaaatt ataattttat tattaatgta gctatgatga ttggagcttc
    16681 attgttatta tataagatgt tatttgaaaa aaataaaaat aatattttct ttttaatcct
    16741 agtaggaatg atatttggaa ccttatttaa aagtgcaaca acatttattc aggtaatgat
    16801 agacccgaat gaatttttag ctttacaaac tagtataatg gcaagtttaa acaatataaa
    16861 cactaatgta ttgttaatag catttataat aattattgcc ataatacctt ttatatatga
    16921 tgaaataaag tatttagatg tattgtcgtt aggtaaagaa caagcaatta atttaggtgt
    16981 agattttgat aaagcagtta aaaaaatgat aatattaata gcaattttag tctcaatatc
    17041 aacagcactt attggaccta tgacattttt aggactttta cttgctaata taacaagaga
    17101 aatttttaaa acatataagc atacatactt gatttcgggt tctatgttga tagggatgat
    17161 tacattgata ataggtcaat tttttataca acatgtattt aagtttgata caactttaag
    17221 tgtagtaata aattttatag gaggaattta ctttattcaa ttactattaa agggggcaaa
    17281 tagatgatag agataaaaaa tatatttaaa agatataaga ataaaaatgt tgtagatgat
    17341 gtctctttta gtatagaaaa gggaaagata acatctttta taggaccaaa tggagctgga
    17401 aaaagtacag tactatcaat agttacaaga cttataggag gtgatggtgg agaagtaata
    17461 atagaaggaa aaagcttgac taattatagc aataaagaat tagcaaaaaa aatagctatt
    17521 cttaaacaat ctaacaatat aactttaaag cttacaataa gagaattagt tggatttgga
    17581 agatttccat atagtgaagg taatctaact aaagaggatg aaaattatat tgatgaagca
    17641 atagaatata tgaagttgac tgatatacaa cataagtatt tagatgaatt gagtggaggt
    17701 caaagacaaa gagcttatat agcaatggta atagctcaag atacagagta catattactt
    17761 gatgaaccac tgaacaattt ggatatgaac cattccgtac agatgatgaa ggtacttaga
    17821 agtctttgtg atgagttaga taagactata gtattagtaa tgcatgatat aaattttgca
    17881 tcatgttatt cagataatat agttgcatta aaaaatggaa aagttgaaaa agtaggtcgt
    17941 acagatgaga tagtaaatga aaaagtttta gaagatattt atgagatgaa ttttaacatt
    18001 aagaatataa atggaaatcg aatctgtatt tatttttaaa atattgtata tgaaaataaa
    18061 cttgctatat aattttttat aaagttttta aattaaaata aagattaata ggagagagga
    18121 aaatattgat atgaataaaa aagcagcaat agtagcagca gtagctatta taggattagt
    18181 gacagtgttt gcattaggtg gaagtaaaaa gaatgaatca aaaacatcag aaaattcaaa
    18241 taataccata aagataactc ataacttggg agaaacagat gtaaaattaa atcctaaaaa
    18301 agtagtagta tttgattatt cggctttaga tacaatggat gcattaggtg tagctgagaa
    18361 tttagtagga cttccaaaag cgagtttacc agcatcttta gaaaaatata aggatgacaa
    18421 gtatgcagat ttaggaggat taaaagaacc agatttagaa ggtataaagt cggcaaatcc
    18481 agatttaatt ataataaatg gaagacaaga agatttttat gagcaattgt caaaaatagc
    18541 accaacaata agtacaagta aagatgataa gaagtactta gaatctgtta aaaataatat
    18601 agacaaaata gcaaaaatat ttggagtaga agaaaaagca aatcaagaat ttagtaaaat
    18661 tgaaaagaaa atagaaactt tgaataaaaa agtaacagat aaaaatttaa atgctttaac
    18721 tataatggta aatgaaggta atttaagtgt atttggtgaa gaatcaagat ttagtatatt
    18781 atacaatagt tttggatttg aaaataaaga taaaaatata aaagaatcaa gtcatggaca
    18841 aaatattact tttgaatata tagctaaaca aaatccagaa gttatgttcg ttatagatag
    18901 aggaatagct actggcagtg atgtaaaaga aagctcaact gcaaaatctg ttttgaacaa
    18961 tgatataata aaatctatgg atgcatataa gaatgataat ataatatatt tagattcacc
    19021 aacatggtat gtaaatgatg gtggtctaac atctttaaat aaaatgatag atgatgcttc
    19081 aaaagcggta aattaataat ccgatataac caaaatgtat tttttataaa atgattatca
    19141 aataaactag gagtttttaa gaactcctag tttatttttt gcataaaaaa gatatagttt
    19201 aaacaataat attatctata ataagataaa tctattcaaa atctaataaa atatgaagtt
    19261 atactagaca aaaagaataa ctcttaaata taattaagtt atataataat atataattat
    19321 aatgatataa ttttaatagg tggtttgtaa ttaaaaagtt tataaattac attaagagta
    19381 ataatctata agaacttata tattagaaaa aattttgtat atatagagga ctctaaagat
    19441 gaactatgta aaacaaggtt tatatattct aattgtatag aaaatttatt gtaaacgcat
    19501 gtatattatt agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg
    19561 ataaaggtgt tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt
    19621 gatttttata agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt
    19681 gattaattat gttgggattg ttcgtggagc tagccaacgt ctaactaaac tagagatgaa
    19741 tcatcaacca aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac
    19801 agggcatgga gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact
    19861 tttattagaa aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga
    19921 acagaataac cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt
    19981 gtttaaaata gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat
    20041 tatatctatt ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat
    20101 ggttagactg gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac
    20161 aggagtaaat actatagaaa aatttaaatt ggatgcaaat caaaatattt gtatacataa
    20221 ggataagaaa tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat
    20281 atttggatat gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga
    20341 catagggaaa tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg
    20401 ctatgtagat aaggaagatt tagtggtaag acaacgagat gtagacaatg aactaattaa
    20461 tataacaaat gaaattaaaa ataaacatag tattactgtt gttagtggaa tttgttgtat
    20521 tgaagatgta aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca
    20581 aaaaactgtt aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa
    20641 aaaaatgatt gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga
    20701 atttatagtt tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga
    20761 agctttagtt cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc
    20821 agtacttgag aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg
    20881 taaatggatt agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt
    20941 ttcaagaata caattttata acactaaatt tgtagaaact tattccaata tacaaaataa
    21001 atacaggata cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa
    21061 tcaaaaacat cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt
    21121 agatgatttt ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt
    21181 tttaaagctt gattctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt
    21241 aataaaaaat attgtacata tgttaaaaga acttaatatc acaactgttg ctgagggcat
    21301 tgaatgcaag gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt
    21361 tgtattttat aaaccaatgc caatattaga atttgaaaaa atattggata aagagtttgt
    21421 ttataatagc taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac
    21481 tgaataaatt tttactttat ttacagaata atgattattt acagaaaagc gcgaaaagtg
    21541 tattccttta agtgtgggat ggatagcaca aatagaggag aaaaaatatt ggccatataa
    21601 gaatgcataa taatataaat atttgaaagg tcagcattat ataaaaatta aaaagaaaat
    21661 aaataatttt tctataaaaa agagactatg agaaaattta ctcatagtct cctaaataat
    21721 gtatttattt tcttcctgca acccatttta atccccaatg aaattcagta tccatttctc
    21781 tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt
    21841 ctatcaatgc tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta
    21901 agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta
    21961 caccttcgta tataaaagca taaataagta ttctttttat atcttttatt ttattaccat
    22021 ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag
    22081 caaaaggtgg attgtctaaa ctaccaaagg catttcctaa tgcttgaaca gcacctttta
    22141 caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag
    22201 agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact aagatttcac
    22261 ctaaattgct attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag
    22321 ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa
    22381 tattcaacaa gattagaata ataatattaa aattatacta aatatactaa aaagtaggaa
    22441 tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat
    22501 cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg
    22561 catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag
    22621 gtacagcata agtagtatct ataaatgcag cagtcacatc tggaagagaa gatggaattt
    22681 gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg tccttaggac
    22741 taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt
    22801 tcttagaaca agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat
    22861 caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag
    22921 ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata
    22981 gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt
    23041 ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct tctaataact
    23101 ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac
    23161 atccaaccac agctaaagtt agtactagac ataataacaa acataataat tttttgaatt
    23221 tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc
    23281 acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact
    23341 catctttcag tataatactg caggatttag caccaatgta tataaaaata tactggttgc
    23401 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
    23461 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
    23521 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
    23581 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
    23641 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
    23701 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
    23761 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
    23821 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcactca
    23881 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
    23941 attctaatat gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
    24001 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
    24061 catttatttc agatgaaaat aaaagagtcc catgatgcat tactttgttt ttgtagttgt
    24121 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
    24181 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctaaaat
    24241 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
    24301 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
    24361 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
    24421 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
    24481 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
    24541 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
    24601 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
    24661 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
    24721 aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat
    24781 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
    24841 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
    24901 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
    24961 taccccgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
    25021 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
    25081 agaagatata gacactattg cactagccaa tgcagaaaaa tttgggattt taactttaat
    25141 accaccatta gttgctataa tactcgcatt tataactaaa aacgtaataa tttctttact
    25201 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
    25261 atttatacag gcatttttag atttagtaga cagagcttta gtatctttag cagacccttg
    25321 gaatgcgggg ataatattac aggttttggc aattggagga gtaataaatc ttgttgctaa
    25381 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
    25441 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
    25501 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat cgagagaaaa
    25561 gctagcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
    25621 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
    25681 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
    25741 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
    25801 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
    25861 tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg
    25921 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
    25981 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
    26041 aaaagaagca tttagtgcat ctgatgcatc tagagcatta ttccagtctg cacttgttgc
    26101 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
    26161 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
    26221 atggtcatta agttcagtaa taaaagagtt aggtacagct aaatttttaa tacacctatt
    26281 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
    26341 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
    26401 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
    26461 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
    26521 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
    26581 tacagcagta atcactattg tatttggata tataccagct ggacttggat tgccaatata
    26641 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
    26701 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
    26761 tatcattatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
    26821 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
    26881 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
    26941 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
    27001 tgtatatata atagattggc actttagtac aaagtgcttt ttttataggc aaaaacagta
    27061 catttatttc tgttttatta tcattcatct attagaatat actttagtta agaatgtgtt
    27121 aataagcagt gttcacattc ttgataaaat gtggaataat atgtattttt actaagacgg
    27181 aaatgaaata attaaaccat taaataatat tataagatga tgaataagta gataaatcaa
    27241 attttgtagg aggatattat ggaaaaatat atatggttgt ttccattact ttttattttt
    27301 catgatatgg aagagataat aggttttgga atatggctta aaaaaaataa atcaatgctt
    27361 gataagaagt atccatttat cagtaaagtt tataaaaatt atagtactga aggcatggca
    27421 tttgctgtgt ttgaagaatt tattttatgc attattttct gtatacttac agttataaca
    27481 gagaatcagt atgtatatct actatggtta ggtagtttta tagcatatac cttacatttg
    27541 gtaattcata tagggcagtc aatcataata agaaaatata taccttcact tattaccagt
    27601 atcatatgct taccaataag catttggtgt ataagtaaga gtatttatat agttgattgt
    27661 gaaatgagta caacaattct gtacagtata attggcatta taattgtagc attgaattta
    27721 aagtttgcac agtcgctgat tggaaaattt acaaagtgga tgtcaaacat ataaatttta
    27781 gtttattgga cagttcaagg aatcagaaat tggaatttta tagtattaat attttatagt
    27841 attaatataa tgtataaaat tttatagcaa tgtatttcat aagtataaag tgactagaat
    27901 tttaagaaat atacaaattc tataaaaact aaataatttc aagtatattt taaaaatctt
    27961 atttttcaca agtttaaaat tgtaaacgat ttcatcaagt ctatgaatat acttttaaca
    28021 tgttatagta aatctcaata taaatttaca tttggttctt tgtgtgttat tttaataata
    28081 aattaagtga aaatgttatt ttacattaat atataatatc tttgcataag agtgaataat
    28141 ataaaagaga aataattgat aataaaaact taacaaaaaa attaatacat aaaaaaaaat
    28201 aaatataatg tgttgacatt taattgaaat attcatacaa taaaattgaa aataaatatt
    28261 tccaaatgag attagcgaga gagtccttta tacgaaaagg cgccgaagaa gaaatttaag
    28321 aaatgccaat ttttagagaa gctttcaggc aaaaatatcg ctaattgatg ggactctgga
    28381 gagacttaag ttaagatact ataagtcacc gaagaagcaa aataatatta aattattaaa
    28441 tctttcaggt atacaaacag ggatatatag gctaggatta ttttccatgc tgatatatct
    28501 tttttttatg aaaaagtaca ggaaataata tcaaagttac ttaaatacag taggtctaat
    28561 tttctaggga gggttatata tgaatgaatt gaaaagagta tctttgtaca atatacacaa
    28621 agagcttgga gcaaaacttg ttgaatttgc tggttgggaa atgcctttag aatatgaggg
    28681 tataaataaa gagcatgaga aggtcagaaa aagtgcaggt atatttgatg tatctcatat
    28741 gggagaggta cagataaagg gagcagagtc agaaaaattt atacaaaacc tagttacaaa
    28801 tgatataagc acattaaaga taaatgacat tatatataca cctatgtgtt atgaaaatgg
    28861 tggagtagtt gatgatttat taatatataa atttggtgaa gaagattatt tattagtaat
    28921 aaatgcaggt aatatagata aagatgtagc atggataata aaacaaagtg aaggatacaa
    28981 tgtagatata aaaaatatat ctagtgaagt gagtcaatta gcaattcaag gacctaaagc
    29041 agaagaaatt cttcaaaaaa tcacagatat agacttaaac tcaataaaat tttataagtc
    29101 aattccatca acaaaagtat gtggttgtcc ttgtttagtt tcaagaacag gatatactgg
    29161 ggaagatggg tttgagatat actgtaaaaa taaatatgta gaaataattt ggaatgaagt
    29221 tctaaaggtt ggaggagaag atatatgtcc agctggtcta ggttgtaggg atactttgag
    29281 atttgaagca gctctacctc tatatggaca tgaaataaat gaacacatat ctccaattga
    29341 aggtggttta tctatctttg tgaaaacaaa taaagaatca tttattggca aatcaatttt
    29401 atcaaaagaa aaagaaagtg gagctaaaag aaagttagta ggatttgaaa tgcaaggtaa
    29461 aggaatgcct agaaatggat atgatataag gattggtgat aaaacagttg gatttgtaac
    29521 aacaggatgt gcatctccaa cgacaggaaa aattttaggt atgggaatta tagattctga
    29581 atatgcaaaa gtaggaaatg aaataggtat agcaatcaga aaaaaagttg ttcctgctgt
    29641 gatagttaag aaaccttttt ataaaaaaca atataaaaaa gataatatta tattaaacaa
    29701 agaaaataaa ttttcatata tacctgctac aagtgaagat aaatcaaaaa tgttaaaagt
    29761 agttggatta aattcagttg atgaattatt ttcagatata ccagaagaag taaaattaaa
    29821 aagagattta aatttggaaa ttggaaaatc tgaattagag gtaagcaaaa tagtaaaaag
    29881 attgtcagaa gaaaatttaa gtttagaaga cttgacttgt tttcttggtg ctggtgcata
    29941 tgaccattat ataccatcaa ttataaaaca tataacttca agatctgaat tttatacagc
    30001 atatactcca tatcaagctg aaataagtca gggaacttta caagtagttt ttgaatttca
    30061 atctatgatt gcagaaatta caggtatgga aattgcaaat gcatcaatgt atgatggagc
    30121 aactgctgca atagaagcat gtataatggc aatgaatcag actagaaaaa gtaaaatagt
    30181 agtgtcaaaa actattcatc ctgaaactct gtcagtatta agaacttact tacaatataa
    30241 agattgtgag atagtagaaa ttgacttttg taatgaatat gggactacag atatagaaaa
    30301 attaaaagct tctgtagaca aagatacagc atgtgttctt atacaaactc ctaatttctt
    30361 tggaattatt gaagaaatgg aagaaataga aaagataact catgaaaata aggctatgtt
    30421 aattatgagt gtagacccaa tatcattagg agttttaaaa acaccaggtg agataggtgc
    30481 agatattgtt gttggagaag ctcaatcact tggaaaccct cttaattttg gaggtcctta
    30541 tgtaggattt ttagctagca aatctaaata tactagaaaa atgccaggaa gaatagttgg
    30601 gcaaagttta gatgtagaag gtaaaattgc atatgtatta actttacaaa ctagagaaca
    30661 gcatgtaaga agagaaaaag caacttctaa tatatgttca aatcaagctt taaatgctct
    30721 tgtagcttca atatatatgg ctactatggg taaagaagga tttaaagagg ttggtatgca
    30781 gtctatgaaa aaagcacatt atacctacaa taaacttgta caaactggaa aatataagcc
    30841 aatatttaaa ggtaagtttt tcaaagaatt tgctgtacaa ggaaatctta atatagaaac
    30901 tataaatgat aaactcttag aagaaaatat attaggaggt tataatttag aatacaatta
    30961 tccagaatta aaaaattcaa ctttgctttg tgtgactgag aaaagaagca aagaggaaat
    31021 agataagtta gttggaataa tggagggatt ataatgaaag aatacaatag tttgttgata
    31081 gatatatcta aaaaaggtag gaaagcatac tcacttccaa agttagatat agatgatata
    31141 aaaatagaag atatgattga cccagatatg gcaagacaaa gtgagttgaa tttgccagaa
    31201 gttggtgaat tagaattagt tagacattat acattattat caaataaaaa ctttggagtt
    31261 gatacaggtt tttatccttt gggttcttgt actatgaaat ataatcctaa aataaatgaa
    31321 gatatggcag cacttccaaa ctttactgga atgcatccat accaatcttc ggatacagct
    31381 caaggttctc tttctttgat gtatgattta tcaagaagac ttgcagaaat tacaggtatg
    31441 gatgaagtta cactacaacc ttcagcaggt tctcatggtg agtttacagg gcttatgatt
    31501 ataaaagcat atcatgaaaa tagaggtgac cataagagaa ccaaagtgat aattccagat
    31561 tctgcacatg gaacaaatcc agcaagtgca gctatggcaa actttgatgt gattcaaata
    31621 gcatctgata aaaatggagc tgtagacata aatgcattaa aagaggtttt gaatgatgaa
    31681 gtagcagctc ttatgcttac taatccaagt actcttggtt tatttgaaaa aaatataaaa
    31741 gaaatagcta ctcttgtaca tgaagcagga ggacttttgt actatgatgg tgcaaacatg
    31801 aacgctatta tggggataac aagacctgga gatatggggt ttgatgtggt tcatcttaat
    31861 cttcataaga ctttttcaac tcctcatgga ggtggaggtc caggagctgg tcctgttgga
    31921 gttaaaaaag aattagtacc ttttttacca ataccagtaa ttgaaagaaa agaagataag
    31981 tatgtactaa attatgatag agaaaaatct attgggaaaa ttaaaaattt ctatggtaat
    32041 tttggagttc ttgtaagagc ttacacatat atactaacta tgggaaggga tggacttaaa
    32101 gaggctagtg aaatggcagt tttaaatgct aattatataa aggaaagtat aaaagatgat
    32161 tatattttgc caatagatac cttgtgtaag catgagtttg tattaggtgg attgccaaga
    32221 ggagaggcca atataaaaac tatcgatatt gcaaaaagac tactagatta tggataccat
    32281 cctccaacaa tgtactttcc tcttattata aatgaggcac ttatgataga acctacagaa
    32341 agtgagagta tagagacatt agatagtttt atagaagcta tgaaaagtgt tgctaaagag
    32401 gcaaaggaag aaccagaact attgaaaact gctccacata atactttggt caaaagagta
    32461 gatgatgcaa gggcagtaaa gaaacctata ctaacatggt cacaaagata aatagttaaa
    32521 aagtagagat ttaaagtata aagagaatta aatttgtaag aaaaagctat ctaacttaat
    32581 ctagtttaga tagctttttt gtatattagt atgtatggta ttttctatat aaagttttaa
    32641 attg
//
LOCUS       DAKBNN010000017        31045 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000017 DAKBNN010000000
VERSION     DAKBNN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.017"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            27..101
                     /locus_tag="OUB02_000813"
     tRNA            27..101
                     /locus_tag="OUB02_000813"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="OUB02_000814"
     tRNA            116..200
                     /locus_tag="OUB02_000814"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="OUB02_000815"
     tRNA            210..293
                     /locus_tag="OUB02_000815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="OUB02_000816"
     tRNA            317..391
                     /locus_tag="OUB02_000816"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="OUB02_000817"
     tRNA            416..492
                     /locus_tag="OUB02_000817"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="OUB02_000818"
     tRNA            502..577
                     /locus_tag="OUB02_000818"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="OUB02_000819"
     tRNA            586..661
                     /locus_tag="OUB02_000819"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="OUB02_000820"
     tRNA            664..752
                     /locus_tag="OUB02_000820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="OUB02_000821"
     tRNA            756..831
                     /locus_tag="OUB02_000821"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="OUB02_000822"
     tRNA            838..911
                     /locus_tag="OUB02_000822"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="OUB02_000823"
     tRNA            926..1002
                     /locus_tag="OUB02_000823"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="OUB02_000824"
     tRNA            1020..1096
                     /locus_tag="OUB02_000824"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="OUB02_000825"
     tRNA            1105..1180
                     /locus_tag="OUB02_000825"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="OUB02_000826"
     tRNA            1188..1261
                     /locus_tag="OUB02_000826"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="OUB02_000827"
     tRNA            1269..1345
                     /locus_tag="OUB02_000827"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="OUB02_000828"
     tRNA            1358..1433
                     /locus_tag="OUB02_000828"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="OUB02_000829"
     CDS             1509..1922
                     /locus_tag="OUB02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884605.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVYGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="OUB02_000830"
     CDS             2231..5662
                     /locus_tag="OUB02_000830"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCT8884606.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLEDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="OUB02_000831"
     CDS             5904..7832
                     /locus_tag="OUB02_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCT8884607.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGALSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="OUB02_000832"
     CDS             8285..8749
                     /locus_tag="OUB02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCT8884608.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="OUB02_000833"
     CDS             8756..9250
                     /locus_tag="OUB02_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCT8884609.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="OUB02_000834"
     CDS             9266..10291
                     /locus_tag="OUB02_000834"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCT8884610.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="OUB02_000835"
     CDS             10278..12725
                     /locus_tag="OUB02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCT8884611.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="OUB02_000836"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="OUB02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCT8884612.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="OUB02_000837"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="OUB02_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HCT8884613.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="OUB02_000838"
     CDS             15440..16534
                     /locus_tag="OUB02_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCT8884614.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="OUB02_000839"
     CDS             16663..18840
                     /locus_tag="OUB02_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884615.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19026..19871)
                     /locus_tag="OUB02_000840"
     CDS             complement(19026..19871)
                     /locus_tag="OUB02_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8884616.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            20088..21254
                     /locus_tag="OUB02_000841"
     CDS             20088..21254
                     /locus_tag="OUB02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HCT8884617.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            21276..22922
                     /locus_tag="OUB02_000842"
     CDS             21276..22922
                     /locus_tag="OUB02_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCT8884618.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            23019..24392
                     /locus_tag="OUB02_000843"
     CDS             23019..24392
                     /locus_tag="OUB02_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8884619.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LDELNTK"
     gene            24837..25790
                     /locus_tag="OUB02_000844"
     CDS             24837..25790
                     /locus_tag="OUB02_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCT8884620.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            25828..26790
                     /locus_tag="OUB02_000845"
     CDS             25828..26790
                     /locus_tag="OUB02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCT8884621.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            26815..27801
                     /locus_tag="OUB02_000846"
     CDS             26815..27801
                     /locus_tag="OUB02_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCT8884622.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            27814..28860
                     /locus_tag="OUB02_000847"
     CDS             27814..28860
                     /locus_tag="OUB02_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCT8884623.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            29027..30757
                     /gene="lpdA"
                     /locus_tag="OUB02_000848"
     CDS             29027..30757
                     /gene="lpdA"
                     /locus_tag="OUB02_000848"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCT8884624.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEKLVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT        12492 a         3407 c         5937 g         9209 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtatatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggtggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagaagata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcactatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcgga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag acataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgcactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaagatc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agcataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagaaaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gagctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gagagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtgtttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattgga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagactaaag aagcttacaa aaataatgat
    17341 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tcgccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa gtggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18421 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggtatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc ttacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18961 attctaatca aattttaaga taaacttttt ttattataaa gataaataac atataacctc
    19021 atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtgt taattaaatc tttataaata tgagaaatac gcacatcatt taaatactct
    19201 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ttgtaagtgt
    19261 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19321 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19381 gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19441 agattagctg caggaggaga actaagttta atttctctca tgttttctaa tataagttta
    19501 actttacttg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19561 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19621 gaggtatttt gataaataca tctagttgag tgaataattc cagaattaat gaacatacac
    19681 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19741 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca
    19801 aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta
    19861 tttctgttca taataatatc ctccttataa ttttaaatta taaatagtaa atttataatg
    19921 ttcaatatta aaaagataat atagactaat aaattagata ataaacatac ttaagaaaac
    19981 ggataaaatc aaatataata actgtatatt aaaataactt aaaaaaatta ttttattata
    20041 agtgcaaaat atacctattt aaataagata agaggggagg attttatgtg tatataaaag
    20101 gtgtaaattt aggaggttgg ttagttttag aaaaatggat gacctcaagt ttatttgaag
    20161 gcacagaagc tgaagatgaa tactatttac caagacagct ttcaagagag gcatatgaat
    20221 ctagaattaa gactcatcgt agtgaataca tcacagagag agattttgca acgattaaat
    20281 ctatgggatt taattcagtt cgtataccag taccatactt tatttttgga gattgtgaac
    20341 cattcatagg atgtgttaaa gaacttgata aagcatttgc ttgggcagat aagtatgggc
    20401 ttagtatatt gatagactta catacagttc caggaagcca aaatggattt gataatggag
    20461 gaatatcagg tatatgtagc tggtcacaaa atccagaata tgtagcgttt acattaaatg
    20521 tattagaaag acttgcaaaa cgttatggga tgaggcatga attgtatgga atccaaattt
    20581 taaatgagcc tataacagaa agaatgtgga acataatgaa tgttccaaat cggtttaagg
    20641 ctgtagataa ggagatggca agggggagta aacctaattc attagagttt ttacgtgatt
    20701 tttatataaa agcatataag gtaatgcgtc cttatatgag agaggaaaac gttatagtat
    20761 ttcatgatgc atttgaatta aaagcatgga aagactttat gagagaggaa gaatttaaaa
    20821 atgttgttct agatacacac caatatttga tgttagctga agcagatgga tgtgaacaaa
    20881 gtattgatag ctatctcaaa tatattagag aaaattatgc taaagatatt ttacaaatgc
    20941 aaaaatattt tcctgttata tgtggggagt ggagcttatt taattcatat gcttgtggta
    21001 tagatacaaa tggaggtcaa tcacctctaa atgggattga atcaaatata gataaattat
    21061 caaaagacga taaaagagaa ttatatagga agatagcaaa agcacagctt gatgcatgga
    21121 gaaatggaag tggtcactac tattggaatt ataagttatt actagataca gttaatgaag
    21181 aaggttggat aggatgggat agttgggacc taggcaaatg tgtagctcaa gaatggtatc
    21241 ctatagaata ttaaactaaa attggaggaa aagatatgat taaaaaccct atacttccag
    21301 gttttaatcc agatccttgt atttgtagaa agggagatga ttattatctt gtagtttcat
    21361 cttttgaatg gtttccaggt atacctgttt atcactctaa agacttaaaa aattgggagt
    21421 tatatactca tattttgaca gatgaaacaa aaatagattt gaagaagctt ccttcatcta
    21481 aaggaatttg ggcaccttgt ttgacttatt gtgaagaaga agatttattt tacatagttt
    21541 atggaattat gaactctatg aatgcaagat attttgatgt tgataattat ttaattacat
    21601 caaaagatat aaagggagag tggagtgaac cagtatatct acactcatca ggttttgatg
    21661 cgtctatttt tcatgatgat gatggtaaaa agtggattgc atcattagac tgggagacta
    21721 gagaaggata tgaaaaacct ggtgttatat gtttagtgga atactgtaca aaaaagaaag
    21781 aaatagtagg atatccaaag agaatatggt cagggggaac tgatagaggt tgtattgaag
    21841 caccacatat aacaaagcgt ggagattact attatataat gtgtgcagaa ggtggaactg
    21901 gatatggtca tagtgtaact atgggaaggg ctaaaaatat atggggacct tatgaaaaag
    21961 attcaatgaa tccaattgta acttcaattc caggcgattt ttatgaaaga catgaccctg
    22021 accatctaaa gcctaaatac tataatccag aatcaaaact tcaaaaatct ggtcatggaa
    22081 gctatataga aacaacatca ggtgaagtat atttagttca tctaacatct agaccatttg
    22141 taccagagtt aaggtgtact ttaggtagag aaacagctat tcaaaaaatg aaatggacaa
    22201 aggataattg gcttcgaatg gaagatgaaa gtaatttggc aaaagaatac gtatcagaaa
    22261 gtaaactaga agaacattta gtttcatcta taccaagttt tgatgacttt gattcaaatg
    22321 aattaggttt acagtactat gctcctagga tttcaccatt atcatttgca gatgttaagt
    22381 ctagaccagg atatgtaagg attagaggac aagaatcaag aacatcatta aataaagtga
    22441 gcatattggc aagaaaatta acttcagtat atgcaagaat aaccactaag atggagtttt
    22501 acccagaggt acatcagcat agtgcgggtt tgataatgta ttatgataat atgaattata
    22561 taaatttaag aaagtattat agtgaaacat tgggtcaaag tgcactatca ataatacatc
    22621 ttgaaaatgg agagaaaacg gaatttttaa atactaggat accaataaaa gatataccaa
    22681 tatatcttcg tctgtatgta caaggtcgta agtcatattt tgaatggagt tatgatgaaa
    22741 aaaactacca gagaatagga aaggtatttg atacaactaa gttttcagat gagtactgta
    22801 aatatggaga atttacaggt acgtttatag gattaacttg tgcagatcgt gtaaagcata
    22861 aacactatgc tgattttgat ttttttgaat atatagtaga tgaatcaaaa gatgtcgatt
    22921 aataaaatca ttactaaaat tttcagagca aaggtaaatc ttatgctctg aaataagaaa
    22981 cattagaagg caaatcttac aaaaaggagt atggtattat gaatacagaa gttaatgtta
    23041 aagcaactac gattagatat gaaaagattt cattttttga aagaatttct tatggttgtg
    23101 gggatttggg gtgcaatatc atatactcag ctatgtcagc atttttgctg ttttattata
    23161 ctaattatgc agatgttagt gcagcagctg taggtagtat tatgcttgta tctaggatac
    23221 tggatgggtt tagtgactta acaatgggaa ttatagtgga tagaactaaa tccaaatacg
    23281 gtaaggctag gccttggatt ttgagaatgg cgattccttt tgctatagca gcagtattat
    23341 tattttctgt tccatcaaat cttggtatca cttctaaatt aatttatatt tttattacat
    23401 ataacttagt ttctactgtt atttatacag caatcaatgt accctatgca actctaaatt
    23461 cacttataac tcaaaaccag tatgaaagag gagtattaag tatatttaga atgatattag
    23521 ccacttgtgg taccttacta ataaatggac ttacactacc tctagttgaa tactttggaa
    23581 ataatttatc agcatggact aaaacattct ttgtatttgg aatagcatct ataatggtat
    23641 tttttataac atttactgga accaaggaaa gagtaaaagc agttaagcaa gataggaatg
    23701 aagtaatacc atttaaaata ggtattaaat ctttatttag aaataagtac tggatacaaa
    23761 taacattatg tttagtttgc atatttattg tatttgcgat aaatggtggt tcatccgtat
    23821 attatgcaaa gtttattttg ggagatgaaa aattatttgg tcctataaat atggtttcaa
    23881 atatatccca aattatagct atgtttatgg ttgctccttt tataaaaaaa tttggaaaaa
    23941 gaaatgtatt aatagtaggt tcaataatat taatatcgtc aaatgttatg tttataatag
    24001 ttggacaaaa tcatatagga gtaatatttg caagtgctat aaagggaata ggaagtgctg
    24061 gaatagcatc aacaatgttt gctattgttt cagatactat tgaatatgga gaatggaaga
    24121 ctggatatag aactgagggg cttataaata gtgcatctag ttttgggttt aaagtgggaa
    24181 atggattagg ttcagcaata ttaggtgcag tattatcaat tggtggttat gtaggaacat
    24241 cagctactca aagtgattta gctatagtat caataaaagt atgttttata tacctaccta
    24301 tatttataac aatattacaa attatcataa tgtcttttta taagcttgat aaagaatata
    24361 gtactatact tgatgaatta aatactaaat aatgaaaaag tttcttataa agtcttaagt
    24421 ttaattaagg tctattgtaa atgttcccta taaaaactat tatatattta tgattattga
    24481 agaattttta gagaagcgaa taataaaaat ataagaagac tttaaatgat gaaatggttg
    24541 gttttttaga aatgatttaa taagttattg ttatttaaag cattataagt ggacaattaa
    24601 tttttaattt atggagatga tatttaacaa ttagaagtaa tatataatat gcactaagaa
    24661 gcaatttttc aactatttat gaaggtggtg gtacaagaaa agtatcacct tttttttgtt
    24721 atacaaaaaa acagttgact ttttatatga accccttcta taatgttact gaaattaagt
    24781 aaaatgattg aacaaatgta aagataattg aaaagcttga ttttggagga aagcaaatga
    24841 aaaaaattgg agacttaaga ttaatgatga agtgttgttc actttattac gaagataatt
    24901 tgaatcaaca ggaaatagca aatcaattgg gaatatcaag accaacaata tcaagaatat
    24961 taaaagaagc ttttgaacaa ggaatagtaa aaatacagat agttgatgtt ttgaaaaatg
    25021 attaccaaaa aatagagaga agtttggaga gaaaatataa attaaaagaa gtaatagtag
    25081 tagatgataa acaggatgca ctaacacaaa aacaagaact agcaagagct gtatctgaat
    25141 atttaactag agtagtaaag gaaaatgata taataggtgt ttcaatagga acaacactaa
    25201 aagaaatacc aagatatgtg gaaaaaagta actgtaaaaa tgttacattt atacctctat
    25261 taggagggat tggagacaat gaaatagata ttcatgcaaa tcaaatagca gtaagtttag
    25321 cgagggcttt tggaggtgat tttaaattat tacatgctcc agctgttatg tctgacttgt
    25381 ctacaaaaga gaaactttgc aaagacgaaa aaattaaaga agtactagat ttaatagata
    25441 aaacaactat agcaattgtg ggtataggaa atcctatgag tttaaactca acaataatgg
    25501 cttctggata catgaatgaa gcagatatag aagatttaaa aaaatacaac tcaataggtg
    25561 caatttgttt acaagccttt gataaagaag gaaaaacttc tatacttgaa tttaatcaaa
    25621 gagtattagg agtgaaactg gaagatttaa aaaagataaa aagaacaata ggtgtcgctt
    25681 caggagatga aaaaatagag gcaataaagg catcattaaa agcgaaattt ataaatagtt
    25741 tagcaattaa tcacagttta gcacttaaat tattagaaga ttgtgactaa tagaaagata
    25801 gataaaagtt aacaggaggg aatgtatatg tcaatatcaa aagaaacatt gcttgaaatg
    25861 tataagagaa tgaatcaggc tagaaaattt gaagagaaag ttagttggtt ttttgcaaga
    25921 ggtatggttc atggaacaac tcacttatct gtaggtcaag aagcgtcaag tgtggcggct
    25981 gtaatggctt tagaaaaagg agatttagta tcattaactc atagaggaca tagtcaattt
    26041 ataggaatgg gaatagacct aaataaaatg atggctgaac ttatgggtaa agaaactgga
    26101 ttttgtaagg gaaaaggagg ctcaatgcat atagcagata ttgagtcagg taacttagga
    26161 gcaaatggag ttgtaggtgg aggtcttaca atagctccag gagctgcact tacacaacag
    26221 tataaaaaaa caggcaaaat agttttatgt agttttggtg atggagcatc aaatgaaggt
    26281 acttttcatg aaggaataaa tttatcttca atatggaaat tgccaataat attttactgt
    26341 gagaataatt tatacggtat gtcaacaagc ataaaacgtc atatgaatat agaaagtatt
    26401 gccactagag cagcttctta tggaatagaa ggaatatcaa tagatggata taacccaata
    26461 gaagtatacg aaacagtaca aaaagctgct gagaagtgta gaagaggaga aggacctgta
    26521 ttaattgaaa gtagaactta tagatggtta ggtcattcaa aatcagatgc aaatgtatat
    26581 agaacaaaag aagaaataga atcatggaaa gcaaaagacc ctattgaatt tttaaaaaat
    26641 tatcttatag aaaataatct atctaatgaa gatgaattag ataagattca agaatttgca
    26701 aagcaatcaa tagaagatgc agtagagttt gctcaaaata gcccaaatcc aaaaatagag
    26761 tctttattag aggatgtata tgcagactaa gaatggtaaa ggaggaaaat aaaaatgagt
    26821 acaagggaat taacatatgc acaagcaata aaggaagcaa tgtcagaaga aatgagaaga
    26881 gatgaaaatg taatatttat gggagaagat ataggaatat atggaggagc atttggtgta
    26941 tcagttggta tgatagatga atttggacca gaaagagtaa gggatactcc tatatcagaa
    27001 gcagctatag caggagcggc agcaggtgca gcagcaactg gtttaagacc tattatggaa
    27061 gtaatgttta tggactttgt aacaatttct atggatgcaa ttgtaaatca agcggcaaaa
    27121 atgagatata tgtttggagg aaaagcacaa gttccaatgg ttgttagatg tccaggaggt
    27181 tctggaactg gttcagcaga gcaacattca caaagtttag aagcttggtt ttgtcatgta
    27241 ccaggagtaa aagtagttgc accttctaca ccagcagatg ctaagggatt gttgaaagca
    27301 gctataagag ataataatcc agtcatattt gttgagaata aattgttata cagaaaaaaa
    27361 ggttttgtgc cagaagatga ttatgtaatt gaaattggaa aggcagatat taaaagagag
    27421 ggtactgatg ttacagtaat tacttatgga agaatgttac aaagtgttga agaagcagca
    27481 gagactctat caaaagaaaa tataaatgtt gaaattatag atttaagaac tttatatcca
    27541 ttagataaag agactatagt aaagagtgta tgtaaaactg ggagagtttt aatttgccat
    27601 gaagcagcta agactggtgg gctaggtgga gaaatatcag ccctgataac agaaagtgaa
    27661 tcatttgatt atttagatgc tccagtaaaa agaatttgtg gaaaagatgt gccaatacca
    27721 tataatccag agttagaaaa agcagttgtc ccaagagttg atgaaataga agaagcaata
    27781 aaatctttaa ttgttagata aggagggata gatatggtgg caaataaaat aaaagccaca
    27841 ccagcagcta gaagtcaggc aagaaaagac aatataaaat tagataggct gattggaagt
    27901 ggagaaaatg gaaggattca tttagtagat gtgttaaatt atttaaaaga taataaagca
    27961 aatactactc ctttagctag aagaatagca gaagatttaa atatagattt agaaactatt
    28021 gtaggtactg gatataatgg aaagataaga aagtgtgatg tggaaaaatt aactggaaaa
    28081 gaaactattg tatcaactaa tacatctaaa tctagtgaga aaaaagagct gaaaattgaa
    28141 aatgaaaatt caagaatgtt taacacagta gaaggaatat ttgaaaagcc aaatccaatg
    28201 agagctactg tagcaaaacg aatgtcagaa agttatttta gtgctcctgt atttacattc
    28261 aatatagaag tagatgctac agaattgaag gtacttagag caaagttaat cgacacagta
    28321 aaagaatcaa ctggagtaaa attaactatg acagatttaa tagtaatggc agtatctaaa
    28381 atacttccaa atcatcaagc cttaaattca gcatggacag atgagggtat atttagatat
    28441 aaagatgtaa atatagctat tgcagttggt ttagatgaag gcttatatgt tccggtagta
    28501 aaaaatgcaa acaaaaagtc tttaaaagaa atagcaaaag aaagcaaaga acttgcggaa
    28561 aaagtaaaga cagggaaact tatgccagca gaccaagaag gtaatacatt tacaataagt
    28621 aatgttggaa tgtatggtat aactacattt acacctataa taaatatgcc ttcaagtgca
    28681 atcttagggg taggtgctac acaagataag tttgttccag taaatggaga ggctaaaata
    28741 aaacctataa tgaacttatc attaacatca gaccatagag taatagatgg aacagtagct
    28801 gcaaaattct taaaagattt aaaagaattg ttagaaaatc ctttatcaat gcttgtatag
    28861 gttttgatat tttgttactg atataaatag tttgaataga ttatattatt ataattgttt
    28921 cagtaaattt aaagctttta tatattaaga attattttta acagtaacta tatgtgaata
    28981 tgatttgctt aattaaaata taagaaaata aataggagga gagcctatgt cagtagaagt
    29041 aataatgcca aaagcaggag tagccatgga agaaggtact atagtatctt ggctaaagca
    29101 agaaggcgaa gaagtaaaga ttggagaacc tatactagaa ataacaacag ataaagttaa
    29161 tatggagata gagtcagaag gagaaggtac acttgctgta atcatacata aagaggaagg
    29221 tgaagtatta ccagtattta cagtaatagg tgtaatagca gaaaaagggg aaaatcaaga
    29281 ggaagtaaaa gctaaatatt tatctggaaa tgtttcaaaa gaagatacag ttaaagagaa
    29341 tcaaaatata gaagttaaag aagaaaaaat aaacaaaaaa gagtgtaatc atgattatga
    29401 tgtagtggta ataggaggag gacctggggg atatttatca gcattaaaag cagctctttt
    29461 aggtggaaga gttgctttag ttgaagaaaa tatactagga ggtacttgct taaatagagg
    29521 ttgtatccct acaaagactt atataaaaac agcagaaata ttagaagaaa ttgaccaatt
    29581 atcaaagaga ggtgtaaaag taactgtaga taaagaacag gacattaaga aggccataaa
    29641 atataaaaat agagtagtta aaaaacttac agctggtgtt ggcggattat taaaaagtag
    29701 agatgtagat gtgtttaatc ttaaagctag tgttaaagaa gagcataaag taattttatc
    29761 agatggaaaa gttttagata cagaaaatat aataattgct acaggttcca aagtaaggat
    29821 attacctata aaaggaatag aatcaaattt aataataact agtacagaag cattagattt
    29881 ggaaacagtg ccagaaaaat tagtaataat tggtggtgga gtgattggtt gtgaatttgc
    29941 agagatattc aattcaagag gttctaaagt aactatagta gaaatggaag atagagtaat
    30001 cccaagaatg gataaagagt taagcgaatc tttaaaatat tctcttagta aaaaaggtat
    30061 aaatgtatta actaaaaaga aagtttctga atttaaagaa gaaggaaata acatattagt
    30121 gtgcattgaa ggtgaagagc caataaaagc agatttatgt ctatatgcta taggaagaga
    30181 agctaatctt tctggcatag aggacttaga tattaaaata gataaaggtt caatagttgt
    30241 aaatagtaaa atggaaacaa gtataccaag catatatgca gtaggtgatg ttactggagg
    30301 tgtaatgtta gctcatgctg cttttaaaat gggagaggta gcagcttcaa atgctctagg
    30361 tgtgaataaa gaagttgact taggtgcatt acctagttgt gtttatacaa ttccagaagt
    30421 agcatcagtt ggtattacag aagaggatgc tagaaaaaaa tataatgtaa aagttggtaa
    30481 gtttaatttt gctggaaatg gaagagcttt agcttctggt caagaacaag gatatgtaaa
    30541 agtagtagca gatgctaaat atggagaaat tttaggtata catatgtttg gatgtggtgt
    30601 agcagaactt ataaatcatg cagcatcatt taaagcgtta gagataccta ctgatgaagc
    30661 aagtgagttg atatttggtc atccatgtac ttcagaagct ttaatggaag ctttggcaga
    30721 tgtaaatgga gaatgtttac acttacctaa aaaataaatc taaacataaa ttattaattt
    30781 aagtctatta ttaatattaa tttatatcat aaaattttaa aacacttact tagccatacc
    30841 ccttattatg caaataattt atttgcataa taaggggtat tttttatgga aaaatataga
    30901 atatagaatc cttgtagttt ttgttgtaca aaaaagatag taatataaaa taacattatc
    30961 attttagaat gatttcaatt gtaattagga aaagtgattt ttttgttttt aaaaaaagta
    31021 ttatttgtaa aatataaaaa aaatt
//
LOCUS       DAKBNN010000018        30666 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000018 DAKBNN010000000
VERSION     DAKBNN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.018"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            131..2032
                     /locus_tag="OUB02_000849"
     CDS             131..2032
                     /locus_tag="OUB02_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8884625.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2035..3078
                     /gene="ypdE"
                     /locus_tag="OUB02_000850"
     CDS             2035..3078
                     /gene="ypdE"
                     /locus_tag="OUB02_000850"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCT8884626.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3093..3398
                     /locus_tag="OUB02_000851"
     CDS             3093..3398
                     /locus_tag="OUB02_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT8884627.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3495..3830
                     /locus_tag="OUB02_000852"
     CDS             3495..3830
                     /locus_tag="OUB02_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884628.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3862..5184
                     /locus_tag="OUB02_000853"
     CDS             3862..5184
                     /locus_tag="OUB02_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8884629.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5194..6264
                     /locus_tag="OUB02_000854"
     CDS             5194..6264
                     /locus_tag="OUB02_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT8884630.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6269..7456
                     /locus_tag="OUB02_000855"
     CDS             6269..7456
                     /locus_tag="OUB02_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8884631.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7653..8525)
                     /locus_tag="OUB02_000856"
     CDS             complement(7653..8525)
                     /locus_tag="OUB02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCT8884632.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNVLWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8919..9110)
                     /locus_tag="OUB02_000857"
     CDS             complement(8919..9110)
                     /locus_tag="OUB02_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884633.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9330..10376
                     /gene="cobT"
                     /locus_tag="OUB02_000858"
     CDS             9330..10376
                     /gene="cobT"
                     /locus_tag="OUB02_000858"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCT8884634.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10408..10968
                     /gene="cobU"
                     /locus_tag="OUB02_000859"
     CDS             10408..10968
                     /gene="cobU"
                     /locus_tag="OUB02_000859"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCT8884635.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10978..11751
                     /gene="cobS"
                     /locus_tag="OUB02_000860"
     CDS             10978..11751
                     /gene="cobS"
                     /locus_tag="OUB02_000860"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCT8884636.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11771..12385
                     /locus_tag="OUB02_000861"
     CDS             11771..12385
                     /locus_tag="OUB02_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCT8884637.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDINLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12452..12631
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12761..14260
                     /locus_tag="OUB02_000862"
     CDS             12761..14260
                     /locus_tag="OUB02_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HCT8884638.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14299..15675
                     /locus_tag="OUB02_000863"
     CDS             14299..15675
                     /locus_tag="OUB02_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HCT8884639.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15676..16647
                     /locus_tag="OUB02_000864"
     CDS             15676..16647
                     /locus_tag="OUB02_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCT8884640.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYVIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16679..17749
                     /locus_tag="OUB02_000865"
     CDS             16679..17749
                     /locus_tag="OUB02_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCT8884641.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17751..18638
                     /locus_tag="OUB02_000866"
     CDS             17751..18638
                     /locus_tag="OUB02_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCT8884642.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18626..19258
                     /locus_tag="OUB02_000867"
     CDS             18626..19258
                     /locus_tag="OUB02_000867"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HCT8884643.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19267..20493
                     /locus_tag="OUB02_000868"
     CDS             19267..20493
                     /locus_tag="OUB02_000868"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HCT8884644.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20490..21098
                     /locus_tag="OUB02_000869"
     CDS             20490..21098
                     /locus_tag="OUB02_000869"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HCT8884645.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            21088..21663
                     /locus_tag="OUB02_000870"
     CDS             21088..21663
                     /locus_tag="OUB02_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HCT8884646.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21656..22408
                     /locus_tag="OUB02_000871"
     CDS             21656..22408
                     /locus_tag="OUB02_000871"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HCT8884647.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22392..23513
                     /gene="cbiG"
                     /locus_tag="OUB02_000872"
     CDS             22392..23513
                     /gene="cbiG"
                     /locus_tag="OUB02_000872"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HCT8884648.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23510..24235
                     /gene="cobJ"
                     /locus_tag="OUB02_000873"
     CDS             23510..24235
                     /gene="cobJ"
                     /locus_tag="OUB02_000873"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HCT8884649.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKSGDTPVGVVKDVGREKEEKLVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24232..24984
                     /gene="cobK"
                     /locus_tag="OUB02_000874"
     CDS             24232..24984
                     /gene="cobK"
                     /locus_tag="OUB02_000874"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HCT8884650.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25003..26481
                     /locus_tag="OUB02_000875"
     CDS             25003..26481
                     /locus_tag="OUB02_000875"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HCT8884651.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26511..27416
                     /gene="hemC"
                     /locus_tag="OUB02_000876"
     CDS             26511..27416
                     /gene="hemC"
                     /locus_tag="OUB02_000876"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCT8884652.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27406..28905
                     /gene="cobA"
                     /locus_tag="OUB02_000877"
     CDS             27406..28905
                     /gene="cobA"
                     /locus_tag="OUB02_000877"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCT8884653.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28922..29887
                     /gene="hemB"
                     /locus_tag="OUB02_000878"
     CDS             28922..29887
                     /gene="hemB"
                     /locus_tag="OUB02_000878"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCT8884654.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30040..30618
                     /locus_tag="OUB02_000879"
     CDS             30040..30618
                     /locus_tag="OUB02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HCT8884655.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12426 a         3055 c         5662 g         9523 t
ORIGIN      
        1 atattaaatt gtggaattaa aatatactag gatataaaat tcttaaataa aaagaaaaga
       61 ttgtttttat aaaaaatatt ttttatacca ctttaaaaaa cacaataata acactgaggg
      121 aggtggggat atgagagaac gcattattct tgaaacatta aaaaatagta gtggttatct
      181 aaacatagat tattttgcag ataagttggg ggtatctact cgaactatca gaaatgaaat
      241 aaaaaagata gaaactataa aagaatgtaa tggttttaaa ttagaatata agtctaaatt
      301 aggatatatt ctaaatatta aagaccaaga taaattcgag cattatctaa gaaacttacc
      361 ctcagattta gttgaaaatc cagaacaacg gttggagtct ataatagtag agcttttagt
      421 aaatgaaggt tataaaacta tagaacagtt aagtaaaaaa tttctagtaa gttcatccca
      481 aataaaaaat gatttaaaaa agatagatga aaagataaag gatacagaat taaaactaga
      541 gcgaaaggct cattatggaa ttaaaataga aggttctata aaagaaattc aaagtatatt
      601 ggtggatagt tattttagag ggaatagaaa tataacagag tacaggaata agtttataga
      661 taatttaaaa ttagctaata ttagaagtac aataaaaaat gtacttaatg aacatgattt
      721 agaagcaaat ttaacagaac ttgaggaaat attagcacaa attattatat tgtatataag
      781 agttagtatg agagtagtgg gaaatgctag cgatttaaaa ttatatacag aagatttaat
      841 tatagataaa ttattagata aaatttttag agataaaaaa tataacctta attatgatga
      901 aaagtattat ttaaaggagc ttataaaact aaagactaaa gataagaaag ccactataaa
      961 gaatattgat aagaataaac ttcaagatat tatgttcgaa ttttttagag agatagataa
     1021 aaaatataac tctaattttt tagaagataa agagtttttt aacttatttt acttacatat
     1081 tgcatgttta atagaacgta taaagaagaa ccacaagata ataaatcctt tttcagttaa
     1141 aataagtcaa cagtatccaa ctgtttttaa cttatctatt caattttcaa aaatcataga
     1201 aaatgagtat catataaaaa taagtcaaga tgaaataggg tttatagcta ctcacattgc
     1261 tgttcctttt gaaaagagag aagaagctaa ttttaataaa aaatataaaa tagcaataat
     1321 atgctcttct ggtggaggta gtgcattttt gataaattta agacttaaag aaatttttcc
     1381 aaatgctgag ataagaaatt tttcattact agaagaaaaa gctgtaatag aatttgcacc
     1441 agatttgatt ttttcaataa caaatctttt atttgaaata aatgctccag ttatacttat
     1501 aaatgaaatt ttagacgaat tggattattt agatattaaa gaaagtgtta gatttgcaga
     1561 ccatatagga aatatatcaa gcccaaaaca atatatatta ggtctatttg ataagaatca
     1621 ctttaggtgt ataaaagata aagctaagta taaggatatc cttgatagta tgtcaaaatg
     1681 tatagttgac gagggagctt gttctccaac ctatcccaaa gatgtatggg aaagagaaag
     1741 ctatttgagt actatttata caaatggggt ggctatacct catccaatcg agatgacagg
     1801 aaacaaaaat ataatatcag tagctttgat acaaactgat attatttatg aaaatagggt
     1861 gcctaaaatt attttcatga tttcattaat aaagggaaat ttagaacttc ataagcaaat
     1921 atctaaatat ttaagtaaga ttatggcaaa taaggatatg gtagacatgc taaataagtc
     1981 acaatcttat gaagagttta tgtataagtt aaaaatctat ctgggaggtt agacatgaat
     2041 atagagtttt ttaagaggtt gtcagaagca gatggtatag catctaatga agaagaggtt
     2101 agacatgttt tattggaaga actaaaagct tatagtgata aaattatctg tgatgggcta
     2161 gggagtatca tcttttctaa aataaaagat gagagtgcac caaacgtaat gatttgtgct
     2221 catatggatg aagttggatt tatggtgaga agtatagaca aacttggtat gattcactta
     2281 ataacaatag gaggagtaaa acctcttgca cagtttgtac aaaaagttag aattacaaca
     2341 aaagaaggga agaaaatccc cgctgtcata aatgctacat ataataatgg taaggctgaa
     2401 aatatatatg cagatattgg agcatataca gaagaagatg tatataacct agggattaat
     2461 gttggggata tggtaacata tacaacaagc tttgaggaat ttactttacc agaccgatta
     2521 gtgggaaagg cttttgatga taggatagga tgttttgtga tgggagaagt tttaaaagaa
     2581 cttaggaaag aaaatctaaa ttgtaatatt cactttgcgg ctacaagtag tgaagaggtt
     2641 ggaataaggg gagcaaagac atcaacacag ctaataaatc cagatatagt atttgtaata
     2701 gatgtagcat gtgctaaaaa tgagtttgtg agagactata caaatcagaa acaaattgat
     2761 aaaggtgtaa tgctaatgca tagagatagg actctagtcc ctagtagaaa aatgatagat
     2821 tatgtaatgg aaattgctga taaaaataat ataccacttc aacatgatat gtttgaatca
     2881 ggtgggactg atggaggaga agcacatctc gtaaatgaag ggaaaccttg tgtagtaact
     2941 tgtgtaccag ttagatatgg tcattgtgca ttttcaatag taagtaataa agatttggag
     3001 aacattataa gattgtatac acagttaatt ttaaattttg atgaagaaca atataaacat
     3061 ataagaaatt ttatctaaag gagaataaga aaatgtcaac agaagtacaa ataatggaac
     3121 ttatatcaac tgcaggagag agtaaatcga aagcttttga ggctttaaaa aaagtgaagt
     3181 caaaagattt taaaggagca aaattgttaa tagaggaagc tcgtaaaata gatattgaag
     3241 ctcacaggat acaaacagaa ttaataactc aagatttaaa tgaagatgtt gaaagtccag
     3301 caatgagtct tttaatggta catgctcaag accactatat gacttctcag ctagcaaaag
     3361 acttgataga ggctttgata tattgtctcg ataagtaatt gtaatgtaga aatagattat
     3421 ttatgtaaaa attcttttaa aagtttagat gcttttaaga gataataaaa atacaaactt
     3481 taggaggaat tattatgaaa atattattat gttgtgcagg tggattttct actaatatgt
     3541 taatgcaaaa tatgaaaaag gtggtaaaag agagtgctaa actcaatgaa aaagattttg
     3601 agtttacagc tataccagca gattcaatag aaagtgaagt tgatgattgg gatatagtac
     3661 ttataggtcc tcaaatagct cacaaaatag acttcttaaa aggaatatta gagccaaaaa
     3721 ataagccttt tgcagtaata gataaagatg tatatggaaa aatggatgga gctacagtta
     3781 tgaagttggc attaataact tataagaaac atcaattagc tcaaggataa taaaaataat
     3841 gagggaaaag ggagattaga tatgttttca aaatttgaag catttatgaa tagagtattt
     3901 atgcctttag ctcataaggt tgacaatcaa cgtcaccttg gttctataaa ggctggtatg
     3961 gttgctatga caccattcac aatattagga agtatatttg caatacttcc agcacttcca
     4021 aatatgcttg gagaaaataa tccaataagc aaattcatac ttaacaattc agcaatattg
     4081 gatttaccag taagtctttc cataggttta ataggattat atgcttgtat gtgtattgct
     4141 tttaatctgg ggaactacta taaattatat ataccaggtt gtgttgcact atcaactttt
     4201 tcctttttat tcctagtagc ctcatttaca ccagatggag ctttagaaat aggaaattta
     4261 ggagctaaag ggctatttac aggtatgata gcagggcttg gttctgtgga gttatataat
     4321 ttttgtaata agaagaatat cacaataaaa atgccagaaa atgtacctga ttttgtatct
     4381 aggtcttttg aattaatacc tactactata atagtttgtg gaacttttat agttgctcgt
     4441 tttatttcat taaaagtatt tggagaatta cctccacaga tacttactca atttttatct
     4501 ccattagtag gaagtatgga taacccatgg tcagttcttg cacttaactt tgctatatgt
     4561 actatattct tttttggaat acattcatca gtatttagtc ctataactag acctataatg
     4621 attacattta tagctgaaaa catagcagct atgcaagctg gagaaacaat acctcatttc
     4681 tttacagcag gagcttcaag tgcattcttt ggatttacag gatgtggaat tagtataggt
     4741 tgtgtaattg catgtatgtt atcaaaaaat gcttcctata agaagattgg acgtgtatca
     4801 ttattccctg cattatttgg gataaatgaa ccaatattat ttggagcacc gataatatta
     4861 aatccaataa tgtttatacc acatgtattt ggtggagcta taataggtac actaccaatg
     4921 ttttttatgc actggggatg gttagcaaag ccagtatttg accctccata tgtaggggta
     4981 ttcttagaag gatttttaac taatggagat tggcattcta tattagcaaa tgcattgcaa
     5041 ttagtattag cagttttatt atattggcca ttctttaaaa taatggaacg tcaacaaaat
     5101 aataaaaaac aaaaagaaga aactaagaat atattctcta aagaagaaga agacttattg
     5161 gatgatttag gattggattt ctaatatttt gttatgaata atacaatgga gagattagta
     5221 tccttaatct ataaaagagg tgttgacgct cttttgatta aagcaaaaaa tactaaaaga
     5281 tatataggag ctttatcagg tagtggtgta tatgtgcttg ttactaagga taaaaattat
     5341 caaattctag atggcagata tattgatgaa gcagataaaa aaacgactgg tttcataaaa
     5401 aggattgtat ctcaaggttc ttatatacca actataattg agttattaaa agaattaaat
     5461 ataaaaaagt tagcaataga aaaccaagct atgagtatac aagagtatct ttcactaaaa
     5521 aatgaaggtt ttgaaatcaa tttaataaca aatgaattat ggaagctaag ggctataaaa
     5581 tctaaagaag aaattgaact tattaaaaag gcttgtgaaa ttacagatga agtatttgat
     5641 gaggttattt ctgaaattaa aaaagatatg actgagctag aagtatcagc tctaattcag
     5701 tatcatgcat taaaaaaagg tgcaagtgga atgtcttttg aaacaatagt ggtatcagga
     5761 gaaagaggtg caatgccaca tggaagacct acaaacaaaa aattgaaagt gaatgaagct
     5821 ataacaatag attttggtgt tgtttatcaa gggtatcaat cagacatgac tcgtacaata
     5881 tctataggta aacctcctaa aattattaaa gagatatatg atgtagtatt ggaagcacaa
     5941 ttatctgcaa tagagtcaat aaaggaagga actagggcaa gtgatgttga caaagtggct
     6001 agaaaaatta tagataaaca tggatttggc gaatatttta atcatggact gggtcatggt
     6061 ataggtcttg gagatggtga agttcccact ttaaatccaa atagtgaaga tatattagtg
     6121 gaaggaatgg taatgtcttg tgagccaggt atttatattc caaatgtagg gggagttaga
     6181 atagaagatg atattgttat aatagatgga aaaggtgttc cactcaataa gacatccaaa
     6241 gagtttataa tattaggaga gtaaaattat gaaatataat tttaataaag tattaaatcg
     6301 taaagaagga aattgtagaa aatggagcaa ccatgtgatt aaagaaaaat ttggcttaaa
     6361 tgaagatgca attcctatgg atttagctga tatcgatttt gagtgtgctc ctgctataaa
     6421 agaatctatc ttaaaaagag catcagtagg agattatagt tatactttta taggagatga
     6481 cttctatgat gctattataa attggaataa aagaagattt gatgtatata tagaaaagga
     6541 ttggataaaa ttaacttttg gtactgtttc aacccttcat tatatagtcc aagcatatac
     6601 taaagaagga gagggtgtat tgataaatac acctgcttat gacccttttg cagaggctgt
     6661 agtaaataat aatagaaagt tgtgttgtag ctcattaaaa ttagataaca atagatatta
     6721 tttagattta gaggatatgg aaaatcaaat gaaatacgag aatataagat tgcttatatt
     6781 ctgtagtcca caaaatccat caggtcgtat atggacaaaa gaagaactat atcaagtgtc
     6841 agagttatgt ttaaagtata atgtaatttt ggtaagtgat gaaattcata gagatataat
     6901 atttaaagat tatagatttg tatctttgtg gaatgctcat ccagaaattt ataaaaatag
     6961 tataatctgt gtatctccta ataaaggctt caacttaggt ggattaaaaa catcatatgt
     7021 attaataaga gatgtaaaaa ttagggagac tttattagaa aggctaaaaa gtaattcaat
     7081 aacttctcca aatgtatttg caataccagc aatagttgca gcatataatg aaagcgaaga
     7141 atggctagat gctatgacta gttatgtaga ggagaatttt gaaatagttt atgatttctt
     7201 tgaaactaat ataccaaaag ctaaagtgat gaagtcagac tcttcttttt tagcctggat
     7261 agatgtaaga gaagtattta gaaatgaaga agaatctaaa gaatttttta gacatgcaaa
     7321 cttaactatg gtagttggaa gttattttgt gaaagatgga gatggattta ttcgtattaa
     7381 tgtggggtgt cctagagaaa cattaaatga ggctctaaat agaatcaaaa aaacgtatat
     7441 aagtatgtat tgttaaatca taaaaaaagt atctatggtt tatataaaaa cacattattt
     7501 agttatcaaa tattactgga attttgtatt ccagtaatat tttttgatta aggaatatta
     7561 ttgaggaata ttaaataata ttatttcata cccttatatt aacttatata aaaatatttt
     7621 attattttgg gaacgggtta gctaaccttt atctagtgta tatttatatt gtaagaagca
     7681 gtaaaaactt ttcctttact aagtttatta ataaacttct tatctttata aattttgtta
     7741 gaatttatac tatcagcaag accataccaa ggttctatac ataaaaaagg agcagtagaa
     7801 ttagtttcac tataataagg agtccaaata ccaactagag ggaaatcttc catagatata
     7861 gttacatatt tactaccatc attattacat atagacactt catctatgtt ggtgtatatc
     7921 aaagcatcat tcttgaatac ttctggattt aactgtaagt tttttaattt tccaataggt
     7981 attatatcat ctgtaaaaga accatttaaa ttgatttttt ctacatcatt tttactttta
     8041 aactcaaggt agtgtttaga aaaatcattt tgttcgtaaa aaggaaggtt gaatgcagga
     8101 tgtccaccta tagaaaagaa tatatcttta gaatcagtat tttttacaat ccattctacc
     8161 tcaatactat tatcatttaa tgtatagttt atgattagct caaaagaata tggatacttc
     8221 tttaaagtag attcattatc tgttaattta taagttacaa atgtatctcc tttatctaca
     8281 atctcaaagt ccatatctct agcaaaccca tgttgagtca tgctatataa attttcttct
     8341 attattgttt cattatcaaa aagtttacct actataggga acaatatagg tgattgtcta
     8401 ccccaatatt ttttattacc atcccaaaga acgtttttgt cgtatttttt agaaaatatt
     8461 ttagttagtt cagcaccaaa actgcttgat tcgataatta aattttcatt ttgtaatata
     8521 ttcatttaaa atctccttta ttatatttta tgagtatatt atactataaa gtataaagat
     8581 tattaaattt tatttgatat tatatttgaa aggtagattt tagtatattt tgaaaatttt
     8641 gttttgaaaa tttgggatta tttaagaaat tttgtattat aaatagaagg aaatagataa
     8701 attgtataga ataaaatata ttaagataag ttaataatgt taaatgggaa gtgagtttat
     8761 ggaaaaacca aatacaaaag ttgttaactt tcaagagtat aaagacagaa aaaaccaaag
     8821 agaagtaaat agagacgaac ttttaaagct tataaaaaaa attgtgactt caaaataggg
     8881 agttgttaaa ctccctattt taatgggttt cataataatt aagacattct aagtccatct
     8941 atataatctt tgaattgtga gaaatctgat tcactactta attctatggt ttctgaacct
     9001 tccgtataaa aattcttaag atagatagta taattattat caacacaatc atgataaata
     9061 catctatatc catcttcata caaacttttt aatttagaaa aatcatccac aatatcatct
     9121 ccttaaagtt cttttaaata tatttttaca aaaagcatct aattgtatgc aaattgacaa
     9181 tgtaaaataa aggaaatgaa cataaaatgt agaataattt ttatatgtat gtaaaaataa
     9241 aaagaaaaag ttgaaatttt tagaaaataa gagtattttt tatctaaaaa gagagtttaa
     9301 aaaataaaaa aatgttggga ggataaaaaa tgagtttatt agaaagtata tcaaaaaaca
     9361 tatattccct agataactct tctattgaaa aaacaaagca gagattggat aggcttatac
     9421 atccaactgg aagcttagga aagatagaag atatttgcat gcaattagca ggaatatttg
     9481 gcaatgaaaa ttttgacaca agtaaaaagg ttataattgc ttttgcggga gaccatggtg
     9541 tatatgaaga aggcgtagca cctgaccctc aaaatataac aaaattacag tttccaaatt
     9601 tttcgaaagg tctatgtgga gttggagtaa taagtaaatt tgtaggggca gatgttgtag
     9661 ctgtagatgt aggaataaac tgtgatgaaa aactggatgg ggtattagac tacaagataa
     9721 gaaaagggac atcaaatatg gcaaaaggtc cagctatgag taaacaagaa gcaattagat
     9781 gtttagagat aggtattgaa atagcagagc agtgtataga aaaagattat aaggttattg
     9841 gtattgggga gatgggtata gcaaatacta ctccaagtac agcaataata tctgtaattt
     9901 cagggtgtga cccgttagaa gttactggta taggtgcagg tcttaagaaa gaaagactaa
     9961 agcataaggc tgaagtaatt agaaaggcta ttgaaattaa taatccaaat ccaactgatg
    10021 gagtagatat tttatctaaa gtaggaggat ttgaaatagg ctcaatggca ggtgttatat
    10081 taggttgtag tgctaatagg attccagtag taattgatgg gtttatttct tatgcagcag
    10141 cattaattgc atataagata aatccaaaaa ctagagaata tatgatagca tctcatttat
    10201 cagcagaatc aggaactaaa agagcattag acatattaaa attagaccct ctattaaata
    10261 tggatatgag attaggtgag ggaagtggag ctgctttagc atttgatata atagaagctt
    10321 caaactatac ttataaaaat atggcaactt ttgatgaaat tgacatggga agataaatat
    10381 aaaaatttaa tatgtaggag tgttttgatg agtaaaatta ttctagtaac aggaggagct
    10441 aggtcaggga agagtaactt tgcagaaaaa ctatgttttg acagaaataa taatacagct
    10501 tatatagcaa cctctattcc atttgatgat gaaatgaaag atagagtaaa aaaacatcaa
    10561 gagaatagac ctagaaactg gaatacttat gaaatatatg aagatatata ttccataatt
    10621 aaggagatat ataaagaaca tgaaactgtt atactagatt gtgtaacatt attagtaaat
    10681 aatctaatgt ttgaacacaa tttaaatata gaagaatcta gtcaagaaga aataaacaaa
    10741 ttagaacaat acataaaaga acaagttgtc aaacttattg aagaaataga aaaaacaaat
    10801 ctatactttg tatttgtaac gaatgagtta ggtatgggaa ttgttcctgg gaataaactt
    10861 agtagaattt atgctgatat tgtaggtcgt ataaatcaat atatagcttc gaaaagtaat
    10921 gaagtttatt ttgtagttag tggcatagct atgaagataa aggagtagta tgcaattatg
    10981 aaaagattta ttttaatact acagtttttg acaaggatac ctataaaatt aaatgtagga
    11041 tttgatgatg aattttataa gtcaatagta tattttccac ttgtaggttt tgtcataggt
    11101 atattatcct atttaatagg atggatttct atgcttttat ttgaaccatt tatagcttct
    11161 ataataataa ctctagctgg tgtcttaatt actggaggac ttcatataga tggattagga
    11221 gatacatttg atgcaatata tagttataga gataaagaaa aaatgcttga aataatgaaa
    11281 gattcaagat taggtacaaa ttcacttcta gcaattatgt ttgtactttt attaaaggtt
    11341 ggattcgttt acgatataat aagtaataat tcattgtggg ttataatttt tatgccaatg
    11401 attgcaaggt taggagtaat gttattgaca tataaaactg tgacaccaag agaaaaagga
    11461 atgggaaatt tatttatagg aaaattaact acaagtatgt taataactgc tataatatac
    11521 acattattga tagttgctct tattactaaa tttatatttt tattacctaa tatagtgttg
    11581 attaaagtat taggttcgat tatagtggtt tttgtattta taatattatt caaaaaacat
    11641 atttataaaa aaattgatgg agtaacagga gatatattag gctgtggaat agaactttca
    11701 gagcttgtat atttaattta catatatttg ttaattttta tgttttttta aagtaaaaag
    11761 tggtgataat atgataagat taatattaat aagacatgca ctgacaaatg ataataaaaa
    11821 aggaagatta tcaggccata taaatagttg tattagtgaa gagggcaaac tacaaataaa
    11881 taaaattacc agatatctga gtaatgaaaa catagataag gtttatacaa caccatctac
    11941 acgtactaaa gatacagtag aaaaaatatc aaaattgaaa ttattagaaa tagaagaaaa
    12001 agaagcttta agagaaatta gttttggaga ctttgaagga agaacttttg aagaaattaa
    12061 aatcaaatat ccaaatgagt ttgaaaaaat gataaaagaa ggtaacaact atagatatcc
    12121 aaatggagaa agtttgatag actcttataa aagagtggca aaagaaattg ataatataat
    12181 tttagaaaat gatattaatt tagatacaaa gacaatactt atatgttctc atgcaggaac
    12241 aataagaaat ataataacac atttgatttc tggtagttat aagtatcact ggaactttaa
    12301 aattgataat gcatctatta cagttttaga aattgatggt ggatttgctg taatagataa
    12361 aatgaatttt acagacttca tataatttaa tcaaaaccaa acattacaat ctgtgtttgt
    12421 aatatatctg taaaattagc aatgaaaata gaatattaga ttaaaattag gttcttaatt
    12481 agatgttaaa agggaaaaag gttagatgcc tttgcagccc ccgctactgt gaaaccaacg
    12541 aaactataaa taccactgtc agtttgatgg gaaggttata gaagtaggat gaagttaagc
    12601 caggatacct gcctaattta atttaaaaca agaggtcttc ggggtgaaag atttttaatt
    12661 aatatatata aaagtcctat ctttagaagt attaggactt tttttaattt gttttttagt
    12721 aaattttaac aaggagggat ggaaagtggg gaaaaaaata atgctacaag gaacagcttc
    12781 aaatgtaggt aaaagtgtct tagtagcagg tttgtgtaga atatttaagc aagatggtta
    12841 tactgttgct ccttttaagt cacaaaatat ggctttaaat tcttttataa ctaaagaagg
    12901 attagaaatg ggaagagcac aagtatttca agcagaggct gcgaaaatag agccaatagc
    12961 tgatatgaat ccagttttat taaaaccttc tggaaataat aaaagtcaag ttattgtaag
    13021 aggtaaagtt gtaggagaaa tgccttcttc taagtatcat gattataaat tggagttagt
    13081 agatgtgttg aaggaaacat ttaatagctt aaattcaagg tatgatattg tagttatgga
    13141 aggggcagga agtaatgcag agataaactt aaaagataga gatatctcta atatgggtat
    13201 ggcagaaata gctgatgcac ctgtaataat agtcggagat attgatagag gaggcgtatt
    13261 tgcctcatta gcaggaacaa tgcttttatt aaatgaagat gagaaaagaa gagtaaaagg
    13321 tgtaataatc aataaattca gaggaaaaaa agagctttta caagatggtg taaagatgct
    13381 agaagacatc ataaaagtac ctgttatagg agtagtacct tatacagata taaaaataga
    13441 agatgaagac agtgtaacta cgagatttaa gaaaaaaatg aataaaagtg atatacacat
    13501 agaaataatt agaaccccac atatgtcaaa ttttacagat tttaatatat ttgagacaca
    13561 agaagatgta agtattagat atgtagacta tggagaatct ttaggaaatc cagatatatt
    13621 aataatacct ggaactaaaa gtactataga cgatttaaca tatataagaa aaagtggtct
    13681 tgaatcgcaa atcaaaaatt tacatagtca aggtaaattg atatttggga tatgtggtgg
    13741 ataccaaatg ttaggtaaaa aattaaaaga cccttatcat gttgaaagtg aaattgaaga
    13801 agttgatgga ataggtcttt tagatacaga aacaacgttt gagaaagaaa agactacaac
    13861 acaagtagaa gctactattg ttgaatgtat cagtgaatac atgaataacc ttaagggaaa
    13921 aaaagtaaaa ggttatgaaa ttcatatggg aattacaaag cgaggtaatg atgtaagaag
    13981 tttagattta ataactaaaa aacttgataa agaagtgaat tatactgagg gaagtgtaaa
    14041 taaagagggg aatgtaattg ggacttatct tcatggaata tttgatgaaa taagctttac
    14101 aagagcaatt ttaaacaata taagaaagaa aaaaggttta ggagaattag aaagcaaagt
    14161 aaactctttt aatgaattta aggataaaga gtatgataag ttggctgatt ttttaagaga
    14221 acatttagat atagagaaaa tatatgagat tatgaaataa tgatgacctt tatttataat
    14281 ttaagtagga ggtgtattat gaaaaaaatt ttaattgcgg gtactaatag tggagtaggt
    14341 aaaacaacaa tatcattagg aataatgcaa gctttggtta agagaaatat gaaagtacag
    14401 ccatataaag tagggccaga ctatatagac ccttcatatc acacttttat aactggaaga
    14461 cattctagaa atttagattc ttatatgtta gatgatgaaa aaataaagta tatagtaaat
    14521 aagtcctcaa aagatgcaga tatttcagta atagaaggtg ttatgggctt atatgatggc
    14581 tttggaatag atttagataa ttgtacaagt tcacatactt caaaggtatt aaagtcacct
    14641 gttatattag ttataaatgg taaatctatg gcagcatcta gtgctgctat ggtacttgga
    14701 tataaggaac tagataaaga tgtaaatata aagggtgtta tagtaaataa tgtaaagact
    14761 aatagccatt atcaaataat taagagttct atagaaaaat attgtaatgt tgaagtattg
    14821 gggtattttc ctccaaataa caaattctct ttggaatctc gacatctagg attagttcct
    14881 agtgtagaaa tgaaatcact aagggaaaaa ttttatacct tagcagatga aattgaacac
    14941 tatatagata ttgacagaat aattgaaata tcagaaagcg atgaatttga gagtgatttt
    15001 gattttcaaa ctttaataga aaataataaa ataaaagaac atgttaataa taaatcaata
    15061 gctatagcat atgataaagc attcaatttt tattatagag aaaatataga gcttttagag
    15121 gaacttggtt tagaaataaa ctattttagc cctcttgaag atgtatcagt gccaaatgca
    15181 gattatattt atattggagg aggttttcca gaaatatttg gaaaagaatt agaagcaaat
    15241 gagtcaatga gaacttccat attagaagca cataacaata atatacctat ttatgcagaa
    15301 tgtggaggac tgatgtacct aggtgagaaa ctattgaatc aagaagaaca agaatttaat
    15361 atggttggaa tatttagtgg aattagtaag atgacatctt ctttaaagag atttgggtac
    15421 tgtattggtg tagccaagga aaatactctt ttatctaaaa aaggggaatc tattaaaggg
    15481 catgagtttc atcattcaat atttgaaagt gatgaaaaat gtgtatattc aatgagaaaa
    15541 gaaagagatg gaaatatagt agaagagtgg gatggtggtt atagtaaagg aaacactttg
    15601 gctacatatc ttcatactca tttttataat aacttaaatt gtatagaaaa ttttttgaaa
    15661 aaaggaaatg aataaatgag tattttatct atttatatag gatatgtgac agacttaatt
    15721 gtaggagacc catattcttt tcctcatcca gtgagattta ttggtaaact tataaatttt
    15781 actcaaggta taataagaaa ggtctctaaa aatgataggc agttaaaatt tgggggattt
    15841 atattatggt ttataaccgt tggaataaca tatttaacaa cttatgttat agtgaagtta
    15901 tttagtttta atatattatt gagtattgta gtgaatagtt ttataattta tacaacatta
    15961 gccacaaaat gtttaaaaga cgaagcatta aaaatttata atgtattaaa aactgaagat
    16021 atagaaaaat ctaggattca gctatcttat atagtaggta gggatactac taatttaagt
    16081 gaacctgaaa taataagagc aactgtagaa actgttgcag aaaatactgt agatggtata
    16141 attgcgccta tgttttatgc atttattgga ggagcaccac ttgctatggc ctataaagct
    16201 atcaatacat tggactcaac agttggatat aaaaataaaa agtataaaga tattggtttt
    16261 gcatctgcta agattgatga tatagccaat tatataccag ctagaatatc tgtcattctt
    16321 atgacaattg gaagtttctt tttaaattat aactatagga actgttttaa aatctctatt
    16381 agagatagaa agaatcataa aagtccaaat tgtgcttttt cagaaggtgc agtatctggt
    16441 gctctaggaa tacaactagg aggtaccaac gtttattttg gggaaaaagt atataagcca
    16501 acaatagggg ataagcttag ggagatagat aaagaagata taattaaaac taataaaatt
    16561 atgtatgcat cttcatttgt atctatgttg gtatttacac taatatatgt gttatgtagg
    16621 atgattttaa ttaacttaat tatataaatt attgtttgtt aacttaaggg ggacatagat
    16681 gaaggattta gggcatggtg ccaatgtaga tgaaatggca agattatatg ggaaaaaccc
    16741 aaaagaaata attgatttta gttcaaacat aaatccaaat gtattaccaa atttagagag
    16801 atacatttta aaaggattag aagagtgtag gaattatcca gatataaatt atacaaactt
    16861 gagagaaaat atatcaaaat atatagatat aaatccagac tttataatac ctggcaatgg
    16921 tgcaacagaa gtgatttatt tgcttatgaa gagtattaaa aaaaaattgg ctataataaa
    16981 tccgactttt tcagagtata gaagaagtgc tgagttaaat aatttagata ttatagattt
    17041 agagttagat ttagaaaata actttaaact taatatagat ataataaaag aaaatataaa
    17101 aagatttgat agtttattta tatgtaatcc taataatccg agtggtaatg tccaagattt
    17161 aaaggaactt gttcatttac tagataaaca taataaggta ctaataattg atgaaacttt
    17221 catggaattt gttgaagatg aaagtaaata tagtttggtt aaatatatag aatcaaataa
    17281 aaatatattt ataattaaag ctgtgactaa gttttttggt atgcctggtt taaggctagg
    17341 atatggtctt acaagcaaca ctgaaattat gaacaaaatt tatgaacaca aagagccatg
    17401 gactatcaat tcttttgcag atatactatc caattttatt tttgaagata aagagtatat
    17461 taaaaatagt aaagaatatt acatagaaga aagaaaatat atgttacaag agttgagaaa
    17521 cataagaaat ataaaggtct atgatacaga tgctaatttt atcttgataa gaatatataa
    17581 aaaaacaact aaagaattga aaaaagattt gtttaagcaa ggaaacatat tagttagaga
    17641 tgcatctaat tttataggat tagatgatag ttttataaga gtggctataa aatctcatga
    17701 agataacaaa attcttatag aaaatatgaa aaatttattg ggtgattaaa atgaaatctt
    17761 atggaatatg cccagcatct tgtggagaat ttgtacaagg tataattgat gatgaagaat
    17821 atttatgttc atatgcaata gatatgtatt ctaaagtgta tattgaagaa aaattagtag
    17881 atataaattt agggagatat aagtcaaggt tagcaattga aaaggtattt gaaaaattta
    17941 atttacctaa aaaatatact aaaaatattt ctttaaatat aaatagtaaa atacctgttg
    18001 gaaaaggaat ggcaagttca actgctgata taggagctac tataaaggca acattatcat
    18061 tgatagataa agatttaagt agcgaggaga tttctaaatt agcagcagaa atagaaccaa
    18121 cagattctat atttatagat aaaaatagta tatttaatcc tctaaatgga actgttataa
    18181 aatacctagg aaatctgacc aatgcgaaag ttgtaatact tgaaccaaac aaagtactag
    18241 atactatgaa gattagattg aggcaagatt ataataaatt aaaagtagag aataaagaag
    18301 taataaaaaa atcatttgct cttttagaag aaggattgaa aaaaaataat ttatctctag
    18361 ttggagaggc ttgtactttg agtagcttag caaatgagaa tatagaaaaa aaagagtgtt
    18421 taaatgaaat aataaaaata tcaaaaaaat atggagcata tggagtaaat attgctcaca
    18481 gtggaactgt agtaggtatt ttaattgata agtctatgaa tgataaaaag atgatagatg
    18541 ccttatgtga atctaacata aattctgtct ataataaaat ttatacacaa aacataatta
    18601 atggaggaat taagggggaa atagaatgga atacataaag aatcctatga aaatagaaga
    18661 aaaaagtttt gaattgatac aagaaataat agacgaaatt agaccagatt ataaatttaa
    18721 gaataatatc gaagaaaaaa taataaaaag agctatacat actactgctg attttgatta
    18781 cttagatata ttaaaaatat cagaagaagc agttgatagt ataatagatg cattaaaaaa
    18841 taatgcaagt atttatactg atacaaatat ggctcttagt ggtataaata aaagaaagtt
    18901 agaatcatta ggatgtaagt ataaatgttt agtggcagat gatgaaactg taaaattagc
    18961 taaagaaaaa ggaattactc gttctatggc agcagtagaa attgcagcta aagaagaagg
    19021 aagaaaaata ttcgttttag gtaatgctcc aacagcttta tataaagtta tggagatgaa
    19081 atcagaaggt agattagatt tagatgcagt tataggagtt cctgtaggtt ttgtaggagc
    19141 acaagaatca aaagatgaag ttgaaaaaac agatatacca tatataattt ctaaaggtag
    19201 aaaaggtggc agtaatttag cagcagcaat agtaaatgct atattatata ctatgtaggt
    19261 acctatatgg aagaatatgt atatatagat ggaaaaaaat atagaagagg atatactaca
    19321 ggttcatgtg caacaggtgc atctaaggct gctgtatata tgttgattac aaaaaataga
    19381 atcaatacta taaatataga tactcctaag gggataccat tactactaaa agttgataat
    19441 ataaatattt ctgatacatt tgtggaatgt tccataaaaa aagatggtgg agatgatata
    19501 gatgcaactc ataccatgga tatttatgca agagctgaaa ttgtagctaa aaatgataag
    19561 aacaaagggt atcttactct aaaggatata gatagtttaa gcaccaatag tgagtgtaaa
    19621 agtgagctct ataagtttat tagggtttat ggcggaactg gtattggtgt ggttacgaaa
    19681 aaagggctta gtgtggatgt tggtaagcct gctataaatc cgactccact taaaatgata
    19741 aatcatgaaa taagaaagct tataggagat aattttgaat ctatacttgg aaatgataaa
    19801 gttttaaaaa taacaatctt tgctccacaa ggagaaactg tagcaaaaaa aacatttaat
    19861 ccaagattag gtatagttgg aggaatatct ataataggta caactgggat tgttgaacct
    19921 atgagtgatg agggttggaa gaagtctcta tctatagaac ttcagatgaa aaaagaacaa
    19981 ggcttagata aaataatatt ggttccagga aatcatggag aacagtttat aagagaaaaa
    20041 ttaaacttag atattaagta tgtagttagg gtaagtaatt ttataggcta tatgattaaa
    20101 gaagctcaaa gaataggata taagaaaatc ttaatggcag gtcatatagg taaatttata
    20161 aaagtatctg ctggtatttt taatactcat agtaaggtag cagatgctag aagtgagata
    20221 ttagtggcaa acttagcttt aatgggagct agatacgaat ttttaaataa aataaatcaa
    20281 tgcgtaacta ctgaagaagc tgtagagcta ataaataata gtgaatatag agaagtatat
    20341 aatatattaa gtaataaatg tagggagaga gtaaagcaat acttgaatga agattcagat
    20401 gatattgatg tggaagtaat catattttca atggataagt ctttattagg taaatcggat
    20461 aatacagatg atttagtgga ggtctttata tgataaacat aataggtcta ggacctggca
    20521 acttagacta tataacaaaa aaaggtgaaa atttaatttc cacttctgat gtattaattg
    20581 gtggtaaaag aaatttagag tctataaaaa attttgaagg agaaaaaata gttctggatt
    20641 ctaatttaag agaaattata gagtacataa ataacaataa agaaaagcaa atatcaataa
    20701 tagcatctgg ggacccatta atatatggga taggtagata tctatctaaa aatattgata
    20761 ataagatgtt aaacatggtt tctgggatta gttcattaca gtacatattt tcaaaaatat
    20821 atgtagatat gaatgatgta tatattacaa gtagtcatgg caaagtacca gattttgatt
    20881 acatattatc acataaaaaa gtatgtatgg ttacagattc aaagatagga ccaaaacaaa
    20941 taagtagaga aataatagat agaaatctaa ataaaattat tgttgtagga gaaaatctgt
    21001 catatgacaa tgaaaaaata acaatagcaa aaccagaaga gataataaga atagacaatt
    21061 ttgatatgaa tgttgtggtg attttagatg aggaatagtg agtttataac aggtaaagtt
    21121 ccaattacta aggaagaggt tagagcaata tcaataagta agttaaattt agtaaatgct
    21181 aaaaacctca tagacatagg ggctggtaca ggaagtgtaa gtgttgaagc tgcatataat
    21241 tatcctaatt taaaggttat atctatagag aaaaatgatg atgctataga gcttatagag
    21301 aaaaataaaa aaaaattcaa cttaaaaaat atagaagtga taaaaggata tgctccaatt
    21361 aatctagggt taaatgttaa agttgagtct atttttcttg ggggaacagg aaataatctg
    21421 gaagaaataa tagaatggtc taaaaaaaat ctcgttacag gaggaagact tgtagctaat
    21481 tttataataa tagatacatt taatcaaact ttaaaactgc ttaaaaagca tggatttaaa
    21541 gaaatagatg tatgtgtttt gaatgtttct aaactagaga aactaggaaa aggtgaatat
    21601 tttaaaccac taaatcctat atatataata tcttgtgaaa aaggggaaaa tgatgatgaa
    21661 taaagtacat tttgtaggag caggacctgg agataaagag ctgataacat taaaaggata
    21721 caaattatta agtaatgcag atgtagtgat atatgcaggt tcacttgtta atcctgaact
    21781 tttggaatac tgtaaagaag attgccaaat acataatagt gcacatatgg atttacaaga
    21841 gataatagat gttatgagag aaggtataga aaataataaa tctgttgtaa gactgcaaac
    21901 aggtgatttt tctatatatg gttcaattag agaacaagtg gaagatttaa ataaattgaa
    21961 tatagattat gactgtactc caggagtaag ttctttttta ggagcagcat catctttagg
    22021 ggttgaatat acagttccag aaatttctca aagtgtaatt ataacaagaa tggaaggcag
    22081 aactccggtt ccagagaaag aatcaataca atcatatgct aagcatcaga catctatggt
    22141 aatattttta tctgttcaag aaattgagaa agttgtgtct aaattactgg aaggtggata
    22201 tccaaaagat actcctatag cagttatata caaagcaact tgggcagatg aaaaaatagt
    22261 taaaggaact ttaagtgata tagctgtaaa agttaaagaa aacaacataa ataagactgc
    22321 tttaataatg gttgggagat ttttaggaga agagtataat aattcaaagt tatatgataa
    22381 ggatttcaaa catgaatata gagggtaata ttgctataat ctgtattaca gaaaatggaa
    22441 aaaaattagc tctaaagata aattctctag taaataactg tgttatatat caagtaaaaa
    22501 ataaaaaaag taatttagtt gttgaagaaa catgtgtaca tacagttcaa aagaagttaa
    22561 gtgattttgt aggagaaata tttagcaaat ttgattacat agtatttata atggctacag
    22621 gtatagttgt acgaagtata gcaccataca taataagtaa gtttagtgac cctgcaattt
    22681 tggtaatgga tgaaaaaggc aaaaacgtaa taagtttatt gagtggtcat attggagggg
    22741 caaacagctt gacacagtac ataagtagtc ttattgatgc taatccagta ataacaacag
    22801 ctactgatat aaatcaaaaa gcttccttag atatgatagc aaaaaaacta aatgcacata
    22861 ttgataattt tagagaaagt gtaaaagaag taaattctat gctagtgaat aataaatgtg
    22921 ttggattata tattgatgaa ggatatcaag ttgatactag gggatttaaa cttatagaaa
    22981 gtaaggatga gttaagagat agtttttcaa acacatatat agaaaaatta gttgtagtaa
    23041 gcaataaaat aaattgtatt gaagatattg agaatttaaa cctagataat aaatttgaac
    23101 attgctatag taccaaaaaa ataataaagg taattccaag agatatagtg ataggaattg
    23161 gctgtagaag aaacacagat agtgagctga tgtataattc acttgaagag tttcttaata
    23221 aacaaaatat agatattaga gcaataaaca cagttggaag tatagaactg aaaaaagacg
    23281 aaaaagcaat aattgaatta tcaaaaatat tgaaggtgcc atttaaaaca ttttcagtag
    23341 atgaaatatc taaggttgat aatctttttg ataaatctga ttttgttaaa aaaaatgtgg
    23401 gcgtgtattc agtagcagaa cctgtagcat atcttttgag caaaggtaat ttgttagttg
    23461 aaaaacataa atataaagga ataacttttt cagtggggag aataaaaata tgatatacgt
    23521 tgtaggaatt ggaccaggaa gtaaagatac aatgacttta gaagcaataa aagcaataga
    23581 agattctgag gctattgttg gatataagac ttatataaaa ctaatagaag aatttataca
    23641 agataaagaa ataatccaaa atggtatgag gcaagaagtt gatagatgta aacaagcagt
    23701 agaggaagca aaaaaaggaa aaaaagtagc agttgtaagt agtggtgatg ctggaatata
    23761 tgggatggca ggtcttattt tagaattaat atctaaagaa gatgaagata taaaagtaaa
    23821 ggtaattcca ggtgttactg caagtattgg tgcagcagct atacttggag ctcctattat
    23881 gcatgatttc tgtcatataa gtttgagtga tttaatgact ccgtgggaag taattgaaaa
    23941 aagattgaga ttggctgctg aagccgattt tgtaatttgt ttgtataatc caagaagtaa
    24001 gggaagaagt gaacatctaa gcaaagcatt taaaataatg ggtgagttta aaagtggaga
    24061 tacacctgtt ggagtggtaa aagatgtagg tagagaaaaa gaagaaaagc ttgtatgtac
    24121 ttttgaaaat atggactttg aaaaagtcga tatgactaca atggttataa taggaaataa
    24181 gtctacctat attaatggtg aattgatgat aactccaaga ggttatacag tatgatttta
    24241 attttaggag gaacttcaga tagtttatct atatgtgata agataaatga actgaaaaat
    24301 caaccatata tcctttcagt tactacagat tatggaaggg aacttgcaca acaacatgca
    24361 gagaatgtaa ttttaggtaa gcttggaaaa gaagatatgc taaaatttat aaaagataat
    24421 aatatagtaa aaataataga tgccactcat ccttatgcag ttgaagtatc caaaactgct
    24481 attacttgtg caaaactatt gaatatagat tatataaggt ttgagagaaa atctttaatt
    24541 gaagaaatag attatgataa taaatttata gtagatacaa ttgaagaagc ctgtgaaata
    24601 gcaaataaaa tagggacaaa tatattcata ggaacaggaa gtaaaaattt gaacagattt
    24661 atagaggaaa taagtgataa aaatttagtt gttagagttc ttccaacgag tgaagtcata
    24721 ttaagttgtg aaaaattagg tttaagtgca gataatatag tagctatgaa aggtccattt
    24781 agccagtata taaatgaaga aacatataaa cattacaata tagatttagt cataacaaaa
    24841 gaaagtggaa tagctggtgg gttcttagag aaggtaaatg catgtaaaac tttcaatata
    24901 ccagtaataa ttataaaaag agaatcgatt aactacgaaa atgtatttaa tgacataaat
    24961 aaaattaaag acatacttgt gtaattttta ggaggagaat atatgaagaa agccttatta
    25021 gtagtgagtt ttggtacaag ctatcatgaa acgagagaaa agacaataga tatatgtgaa
    25081 gataaaataa aaaattcgtt aaaaagccat gattttttta gagcttatac atcaaatatg
    25141 ataataaata aaataaaaaa aagggatgga atagaaatag ataatccaat acaggcttta
    25201 gataaaatat atgaacaagg atatgatgaa gtaataatcc aaacacttca tattatatgt
    25261 ggcgaagaat ttaacaaatt aaaagaacaa gtagatggat attcttctaa attcaaaaag
    25321 attgtattag gaagaccttt gcttacacat attgaagatt atcaggaagc tgtagaggca
    25381 attaagcatc aaattcctca tatggaaagt aatgaagcag tagtatttat gggacatgga
    25441 acacttcatg aatcacattc agcgtatcca gcattggaat acatgcttag ggataatgga
    25501 ataaatgctt atgttggtac agttgaaggg tatccagaaa tagaacatgt aataagaaga
    25561 ttaaaagaag gaaatataaa aactgtaaat ttgatgccat ttatgttagt tgcaggagac
    25621 catgctataa atgatatggc aggagatgaa gaagattctt ggaaaacaat attagaagaa
    25681 aatggattta acgtaagagt tcatttaaaa ggactaggag aaaattctta tatacaagat
    25741 aaatttgttc gtcatgctgt aaaatgtgca gagaatgcga agttctatgg tataggagca
    25801 ggaccaggag atggttcttt acttacgata aaagcggtta atactttaaa gaaactggat
    25861 attctataca ctccagaatc aaaaaagggt ggagatagtc tagctttatc aatagttagt
    25921 gaatatttac ctgaatcagt ggaaataaag tctaggcatt tcccaatgag ttttgatggg
    25981 aatgaaaaaa gtttagcttg ggataatgtg gcacaagaaa ttatatcaga tgttaatgat
    26041 ggtaaaaatg taggatttgt tacattagga gaccctatga tttatagtac atatgtatat
    26101 ataatgaaaa gaatattagg agaggtagaa gtcgaaacta taccaggaat ttcatcattt
    26161 tccaatatag catctaatca gaattttcca ttagtcatgg ataaagaagc tttagtggta
    26221 attccttgta ctatggaaga tgataagata gagtatgctt tgcaaaatta taattcaata
    26281 gtattgatga aagtatataa aaattttaaa gaaataataa aaaaattaga aaagtatgat
    26341 ttaatagaac atgctatttt agtaagtaat tcttcccaag aaagcgaaaa tgtgtttaca
    26401 gatttaaaag aagcgcacct tgaagataag atatcttatt tttctactat actagtaaat
    26461 aaagataata aaataaaata ggtgaaagca atatttaaag gagacttaat atgaatatag
    26521 tggttggaac taggggtagt aatctagcgc ttatacaaac agaatgggta ataaatgaat
    26581 taaaaaagaa gtatccagaa attagttttg agattaaaat cataaagaca aaaggagatt
    26641 taattcaaaa tgtttcactg gataaaatag gtgataaagg attgtttgtc aaagaaatag
    26701 agcaacaatt attagatgga aaaatagaca tagcagtaca tagtatgaaa gatatgcctt
    26761 cttatttagc agatggacta aaatttgctc atacgcctaa gagagaagac ccaagagatg
    26821 ttctaatact tagagaagga tataaaaatt tagatgattt acctcatgga gcagtaatag
    26881 gaacaggaag taaaagaagg aagtttcaat tattgaaaca aagacctgat ttaaatatag
    26941 tacaagttag aggtaatgtg gaaactagga tacgaaaaat aaaagatgaa aatatgcatg
    27001 gtattgtgtt ggcagcatca ggaataatta gagctaattt gcaagataaa ataagttctt
    27061 atttacctgt tgatgttgtt attccagcac ctgcccaagg agctttagct atagaaatta
    27121 gaagcaatga tagtacgata gagggaatag taaattcact gaaggatgag aatacagaaa
    27181 tccaaatatt ggctgaaaga ggttttttag atggtgtaaa tggaagttgt catattccaa
    27241 tggcagctta ttgtgaaata atccaagata aaatacatct tactggctta tatggagatt
    27301 cagaaggaaa aaaagtagta atcaagtcta tagatggaga tatttcttct cctagagagt
    27361 taggtttaaa gttagctaaa cttgtattga aggagtatga gaattatgaa gggtaaggtt
    27421 tacttagtag gggcaggacc aggtgattat aaactcatga ctttaaaagg tttagagtgt
    27481 ataagaaaat cggatgtaat agtatatgat agattagcaa atagtagtta tttaagagaa
    27541 gctaagcctg attgtgaact tatatatgta ggcaaggcat caagtaatca tattttgcca
    27601 caagaagata taaatagaat tatagcagaa aaagctaaag aaggaaaaat tgtaactcgt
    27661 cttaaaggag gagaccctta tgtatttggc agagggggag aagaagcaga gactttagta
    27721 gatgaaggta tagaatttga agtggttcca ggtataactt ctgctatagg aggtttatgt
    27781 tatgcgggta ttcctataac acatagagac catgcttcgt catttcatgt aataacaggt
    27841 catttgaagg gtgatgatag tggagaatta aactggaatg ctcttgcaaa taacaaaggt
    27901 actcttgtgt ttttgatggg aatttcaaac ctagagaaaa taagtgaaaa cttaatgaaa
    27961 gaaggaaaag ataaggatac acctgttgct cttataagtt gggcaactag atataatcaa
    28021 agagttgtta catctacact tgaaaatgtt tatgaaactg ctattaaaga agaagtaaaa
    28081 ccgccaactc ttatagtagt tgggagtgtt gtaggactta gagaaaagtt gaacttcttt
    28141 gaaagtaagc ctttatttgg aaaaagtatt gctgtaacta ggtctagaaa tcaaaatagt
    28201 gtattggtag aaaaaataat ggatttaggt ggaaatccta tagaaatacc tactattaag
    28261 gtagaaaaaa tacaaaataa tattaactta gaaaatgaaa taaaaaatat aaataagtat
    28321 aattatctaa tactaactag taaaaatgcc gtagaaatct tctttgaaaa gatatatgaa
    28381 atgaattttg atttaaggat attatctaat ttaaaggtat gtgctatagg ttctgctaca
    28441 tcaaatgagt tgaaaaaaag aggaattata gcggatatag taccaaaaaa atttgtagca
    28501 gagtcactgt atgaagagtt agcgcctatt ttaaatgatg acgataatat acttatacct
    28561 agagcagaaa atgctagaga ttatctggtt gacaaactta aggctatttg tgaagtaaca
    28621 gaagtacata tatatagaac agtaatagat gaggagaaaa aaggtgagat attagatatt
    28681 ttaaattcta atgacataga ttatataaca tttactagtt cctcaacagt aaataatctt
    28741 gttgaaatta taggaaaaga aaatattaaa atattagaaa aaataaaaac actatctata
    28801 ggtcctataa ctacagagac aataaatagt ttgggaataa aattgtacaa agaatctgct
    28861 acatctacta tagattcgtt agtagattta attataaaaa attaattttg gaggcttgaa
    28921 tatgttaaga agaccaagaa gactaagagc aagtaaagca atcagaaatt tggtaagaga
    28981 aactaaggtg aatgtagaag acttaatata tccactgttt gtagtagagg gggaaaatat
    29041 aaaagaagaa attaaatctt taccagatgt ctatcattta tcaatagata tgctagaaga
    29101 cgaaataaaa gaaataagtg agttgggaat tgaacatata atgttatttg gtgttccaga
    29161 tgaaaaagat gagtgtgctt cacaagcttt taatgataat ggaatagttc aaaaggcaat
    29221 tagaaaggta aaagaaataa attcagatat aaatgtaata acagatgtct gtatgtgcca
    29281 atatacaagt catggacatt gtgggatttt aactgatact ggttatgttg ataatgataa
    29341 atcattggag tatttggcta aaatatctgt aagccatgct aaagcaggag ctgatatggt
    29401 tgctccatct gatatgatgg atggaagaat acaagcaata agagaagctt tagatgaaaa
    29461 tggattagaa aatgttggaa tcatggctta tagtgttaag tatgcttcaa attattatgg
    29521 accttttaga gaggcagctc attcagcacc atcttttgga gatagaaaaa catatcaaat
    29581 ggacccagca aatagcaatg aagctataat agagactgaa cttgatatat tggaaggtgc
    29641 agatatactt atggttaaac ctgcattatc atatttagat attataagaa gagttaaaga
    29701 taattttgac ttacctctag cagcttataa tgtaagtgga gaatactcta tgcttaagtt
    29761 tgctgtgaag gaaggtttat taaatgaaga tgtaattctt gaatcaatta catctataaa
    29821 aagagcagga gcagatataa taataactta ttttgctaaa gacattgcta aactgttgaa
    29881 aaaataaatt ctagtagtat aaagaatatc aaaaagtata ggaattgtaa ttaactatta
    29941 tacagtagtt ttgtttagac atttattgaa gaataataga aactaatttg caatttctat
    30001 actttattta tgtaactaaa taaaatttgg aggtagaaga tgctatatcc tattaattta
    30061 aaattagatg agttagatgt agttataatt ggtggtggag aagttgcata taggaaatgt
    30121 aaaaattttt tagagtttaa caaaaatgtt actatagttt ctaaacaaat tttaaataaa
    30181 ttttatgatt taaaaggaaa tataaaaata ataaaagatg attataaaga aatatatata
    30241 aaagatgctt ctgtaataat tgctgccaca aataatagag agttaaatat ggaaatagga
    30301 ttatattgta aagcaaaaaa taagctagtg aatgtagttg ataatataga aatatcgaac
    30361 tttactgttc catcatatat aaaaagagga gatttactta taagtgtgtc tactgggggc
    30421 aagagtcctt cattatcatc aaagataaaa aaagaaattg aagagagata tactgaagat
    30481 tatgaggagt atttaaatgt attaggagat ataagaaaaa aggttataaa aaaatacgaa
    30541 gataaaagta aaagaaaaga tgtgcttaat atgttggtaa cacttgacct agaagaactt
    30601 aagaagtttg atatataaaa aataaaataa aaaatgttaa aaaggtgttg acggattatt
    30661 tttaag
//
LOCUS       DAKBNN010000019        30491 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000019 DAKBNN010000000
VERSION     DAKBNN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.019"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            133..483
                     /locus_tag="OUB02_000880"
     CDS             133..483
                     /locus_tag="OUB02_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCT8884656.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            502..2145
                     /locus_tag="OUB02_000881"
     CDS             502..2145
                     /locus_tag="OUB02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCT8884657.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2226..4283
                     /gene="recG"
                     /locus_tag="OUB02_000882"
     CDS             2226..4283
                     /gene="recG"
                     /locus_tag="OUB02_000882"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCT8884658.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4342..4896
                     /gene="rsmD"
                     /locus_tag="OUB02_000883"
     CDS             4342..4896
                     /gene="rsmD"
                     /locus_tag="OUB02_000883"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCT8884659.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4898..5395
                     /gene="coaD"
                     /locus_tag="OUB02_000884"
     CDS             4898..5395
                     /gene="coaD"
                     /locus_tag="OUB02_000884"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCT8884660.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5399..5923
                     /locus_tag="OUB02_000885"
     CDS             5399..5923
                     /locus_tag="OUB02_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884661.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6267..8186
                     /locus_tag="OUB02_000886"
     CDS             6267..8186
                     /locus_tag="OUB02_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8884662.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8199..8486
                     /locus_tag="OUB02_000887"
     CDS             8199..8486
                     /locus_tag="OUB02_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884663.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8619..9878
                     /locus_tag="OUB02_000888"
     CDS             8619..9878
                     /locus_tag="OUB02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCT8884664.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9881..10621
                     /locus_tag="OUB02_000889"
     CDS             9881..10621
                     /locus_tag="OUB02_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8884665.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10944..12254
                     /locus_tag="OUB02_000890"
     CDS             10944..12254
                     /locus_tag="OUB02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCT8884666.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12352..13269
                     /locus_tag="OUB02_000891"
     CDS             12352..13269
                     /locus_tag="OUB02_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT8884667.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13285..14094
                     /locus_tag="OUB02_000892"
     CDS             13285..14094
                     /locus_tag="OUB02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT8884668.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14152..15900
                     /locus_tag="OUB02_000893"
     CDS             14152..15900
                     /locus_tag="OUB02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HCT8884669.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16162..17184
                     /locus_tag="OUB02_000894"
     CDS             16162..17184
                     /locus_tag="OUB02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HCT8884670.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            17189..19495
                     /locus_tag="OUB02_000895"
     CDS             17189..19495
                     /locus_tag="OUB02_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT8884671.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19467..20582
                     /locus_tag="OUB02_000896"
     CDS             19467..20582
                     /locus_tag="OUB02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884672.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20600..22018
                     /locus_tag="OUB02_000897"
     CDS             20600..22018
                     /locus_tag="OUB02_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HCT8884673.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22033..23241
                     /locus_tag="OUB02_000898"
     CDS             22033..23241
                     /locus_tag="OUB02_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884674.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23315..24703)
                     /locus_tag="OUB02_000899"
     CDS             complement(23315..24703)
                     /locus_tag="OUB02_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCT8884675.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25044..26378
                     /locus_tag="OUB02_000900"
     CDS             25044..26378
                     /locus_tag="OUB02_000900"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCT8884676.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPSIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNISK"
     gene            complement(26520..27437)
                     /locus_tag="OUB02_000901"
     CDS             complement(26520..27437)
                     /locus_tag="OUB02_000901"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCT8884677.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27645..28529
                     /locus_tag="OUB02_000902"
     CDS             27645..28529
                     /locus_tag="OUB02_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HCT8884678.1"
                     /translation="MKVLKKITILLLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28742..>30491
                     /locus_tag="OUB02_000903"
     CDS             28742..>30491
                     /locus_tag="OUB02_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HCT8884679.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGF"
BASE COUNT        11974 a         3246 c         5451 g         9820 t
ORIGIN      
        1 aacttttcta ggtttaaatt ataccatatt tatagtatga tatgattata attttatata
       61 ataaataagt aaaaatagta aaaaataaat taagaaatgt ttttaataaa aataagctta
      121 agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg gaaatataga aatagataat
      181 caagtaatag ctcaagtcgt atatagagct gcaatggaaa gttatggctt agtaggttta
      241 gcatataagt ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac taaaggtgta
      301 agagttgagg aagtagaaga agatgccata gcaatcgaac tttatgttat aatacaatat
      361 ggtacaaata tatctactgt tgccaataat ataatagata gagtaaaata tgtagttgaa
      421 aaaatgactg gcgtaagagt tactagaatt gatattaatg tacaaggaat aagagttaag
      481 taatttcata ggaggaaaca aatgattcaa tatatagatg gtaaaaggtt aagggagatg
      541 tttatttcag gtgcaaacaa tctccaaaac aataaagagt tagttgacaa gttaaatgta
      601 ttccctgtac cagatggaga tacaggaact aatatgtcat taacaatatc ttatgcctta
      661 aaagaattag caaaagttga aaatgataat atatcagata tcggaaaagc attatctaaa
      721 ggttctttaa tgggagctag aggaaactca ggagttatac tttcacaaat aatcagaggt
      781 atagctaaat ctatagaagg aaaaagtaaa ttatcaacag aagatttagc aaaagctttt
      841 aaaaatggtt cagacacagc atataaagct gtaataaaac caattgaagg aactatactt
      901 actgtagtta gagaaagtgg agagtttgca ataaaaactg cgaaaaaaga gaaagatgta
      961 gtaaagtttt taagtatgct tgtgaaggaa tcaaattctt cattagaaag aactccagac
     1021 cttctaaaga atctaaaaga agctggtgtg gttgactctg gtggaaaggg tcttgtactt
     1081 atatatgaag gaatgttagc ttctataaaa ggcgataaca tagaaataaa aaatgttgac
     1141 ctagatacta atatttcaac aagtatggat tttacaaaga gtactactag cacagatgat
     1201 ataaaatatt gctattgtac agaatttatt ttagaaagtt ctaaggtaga agatactaaa
     1261 ataagagaca taatgatggc ttatggtgat agcctagcag tagttggaga tgatggagtc
     1321 ataaaggtac atgtacacac taatgaccca ggaaatgtac ttcaagaagc tctaaagtac
     1381 ggtcaattat taactataaa aatagaaaat atgaagttac aacatgaaaa tacattactg
     1441 gatgtagaag aaaagaaaga aaatgatagt gaacctttag aagaagaaaa agagtttggg
     1501 tttatagcta cttctatggg tgaaggtttg gcaaatatat ttaaagattt cggagttgac
     1561 catataatag aaggtggtca aactatgaat ccaagtactg aagattttat gaatgctata
     1621 aagcatataa atgctaaaaa tatatttatt ttcccaaata acagcaatat aataatggct
     1681 gctaatcagg caaaagaatt gagtgataaa aatataatag ttatacctac aaaaaatacg
     1741 cctcaaggat ttgctgcatt agtaactttt aatggagaat taagtgaaga tgaaaataaa
     1801 gaagctatga tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt tgctgttagg
     1861 gatacagtga tgaacgaaat tgatgttaaa gaaggtaata taattggaat tgcagaaggg
     1921 aatttattat cagcaggaga ttatgtggat gaagtaacat caaaccttat tgaaaagttg
     1981 gttgatgaag atactgctat aataacatta ttctttggtg aagatgttac agaatctcaa
     2041 gcaaatgaac tacgtacttc tttagaagaa aagtttgaag atgtagatgt agagttatat
     2101 tatggaggac aacctcttta ttattactta atttcagttg aataatgatt ataagaacct
     2161 gtatgaccaa gtagctttta taaaattggt tgtacaggat ttttgtgtag taggtgatat
     2221 atttgttgga tttatataaa gatgttcaat atgttaaggg aataggacct aaaaaagcag
     2281 ataaattaaa taaactgggt atatttacac taaaagacct attgtattat tttccaagac
     2341 aatttgaaga tagaaataat ctaaagaaaa tagcacaatt agaagatggt gagaaagtta
     2401 cgataaaagc agtaatatca agtataaata catttagccc aaaagaaggt atgactttaa
     2461 caaagattga tgtaaaagat gagacaggct cggctaaatt agtgtttttt aataagagtt
     2521 atataaaaaa tacttttcgt ccaggagatt caatattagt ttttgggaag gttaagaaaa
     2581 aatttaataa cttagaactt acatcatgtg aattagaata tcttactaat tcacctaaga
     2641 atacatgtag atttatgcct gtgtaccagc ttacatacgg tgttacaaat aaagaaatta
     2701 tgagtataat tagaacagta cttgaagata aagaattaat tatacaagaa tatatgccac
     2761 aaagaattat agaaaaatat aggctatgta gtatagattt tgcagttaga aacatacatt
     2821 caccaagtag taaggaatca ttaaaaatag ctttatatag aattgtattt gaagaattac
     2881 ttatactaca actagggttg tttgtattta aaagtggcag aaataaagaa gatggaataa
     2941 agtttgaaac aagtaaagac ttaaaaaaaa taataagtgc tttaccattt aaactgacta
     3001 aagctcaaaa cagggcatta gatgaaatta tacaagatat gaatttagag aaaataatga
     3061 atagattggt acaaggtgat gtagggagtg gtaaaacagt agtagctctc ttagcacttg
     3121 caaattgtgt tttaaatggt tatcagggcg ctttgatggc acctacagag atattagcag
     3181 ggcaacatta tatttcactt actgagtctc taaaagattt tggaataaat gtaggtctat
     3241 taataggtag tcttacaaaa aaacaaaagg atacagtatt ggaacaaata aagaataatg
     3301 agattgatat attaataggt acacatgcac taattgaaga taaggttgaa tttaataata
     3361 ttggtttagt tataacagat gaacaacata gatttggtgt tatgcaaagg agcaaacttt
     3421 cattgaaagg agcaaatcct gatatattag taatgacagc aacaccaata ccaagaactt
     3481 tagcacttat actctatgga gatttagata tttctattat agatgaactt cctccaggaa
     3541 gacagccaat agaaacaatt gctattgaaa aaagtaagcg agatagagca tataataacc
     3601 ttgtaagaag agaggttgaa tcaggaagac aagtgtacat tgtatgtccc ttggtagaag
     3661 aaagtgaagc catagaagcc aaatcagcag ttgaacttgt tgaggagtta agagcagaat
     3721 attttcacga cttaaggctt gggttgcttc atggtaaaat gaagtcaagt gaaaaagatg
     3781 aagtaatgag actttttaaa aataaagaaa tagatatttt agtttcaaca acagtaatag
     3841 aagttggtgt caatgtgcca aatgcaacat taatgataat agaaaatgca gaaagatttg
     3901 gacttgcaca attacatcag cttagagggc gagttggaag aggtagtcat aaatcgtatt
     3961 gtgtactaat ttatgattct aaaacagatg tatgtagaca aagaatggct ataatggaag
     4021 agaccaatga tggttttaag atttcagaga aggatttaga aataagagga cctggggaat
     4081 tttttgggac aaggcagcat ggtcttccag aacttaaggt agcaaacttg tttaagcata
     4141 taaaaatact gaagttagca cagcaagaag ctcgatatat attgggtgaa gataataatc
     4201 tacaattaaa agaaaacatg gcactcaaaa aagaaattat agataaattt aaagatacat
     4261 tgaaagaaat ttcattaaat taattttaaa ttgtgttaaa atattactaa tgtaataatg
     4321 tgctgaaggg agattgtcat attgagagta atttcaggaa aagcgagagg attaaaacta
     4381 aatactccaa aaaatgaaga tgtaaggcca actacagata gagtaaaaga gtctctattt
     4441 aatatgataa attcatatat aatggaaagt gaagttctgg atttatttgc tgggactggt
     4501 tctttaggaa tagagtgctt atctaggggt gccaagtcat gtacttttgt agatatcagt
     4561 aaggaaagca tagatattgt taagtccaat ataaaaaagg caagagttga aagtgaaagt
     4621 attattttaa accttgattt taagactgca atagataaac ttaaattaca aaatagtaaa
     4681 tttgacataa tttttatgga ccctccgtat tataaaaata tgtttataga agctattgaa
     4741 aaaattgata attctaatct attgaacgaa gatggaataa tagttgtaga acatgatacg
     4801 aatgatttat ttccagataa gatatgtaaa ttggagaaaa ctagagataa gaaatatgga
     4861 aatactacat taacttttta taagatggag gcataatatg gaaaataaac ctagaaaagc
     4921 tatatttgca ggaagttttg acccaattac taatggacat ttagatatta tttgcagggc
     4981 ttcgaagtta tttgacgaat tacaaatagg agtattaaat aatcctaata aaaagggtct
     5041 atttagcttt gatgaaagag taaaacttat agaaaaaagt acaagccatt taaataatat
     5101 aaaagttgtg acttttgatg gtttattaat aaattactgt caggaaaatg ggattggagc
     5161 attagttaga ggtgtgagaa gtggtgctga tgttgattat gaacttcaga tggcacatat
     5221 gaatagagag cttaatccag atatagagac tataatttta ccaagttgta caaaatattc
     5281 atttataagt tcatctttaa taaaagaggt tttgctcttt gatgctgata taaaaaattt
     5341 ggttccaaag attgttttgg aagaattaaa gaaaaaaact agtgggggta attgatatat
     5401 gaagatagat ttagaaatga ttgaactatt tgagcagtta gaggagatat ttagaagtgc
     5461 ttctacaatc cctttctcac ataaatgcac agtggataaa gatgaggttt tagcaataat
     5521 taatgatata agaactttaa taccagaaga agtaacacag gcagtatgga ttaataaaga
     5581 aagaaataaa ataataagtc aagctaagca agatgctaca aatatagttg aacaagcgaa
     5641 aaaagaagct gagagaatac aacaagaata tcaagataat atagaggaat taaagaaaaa
     5701 ttctgaagat gtagtaaagg catatgtaga atccagcgaa cctgttgttc aagcagacca
     5761 aagagcaaaa gatatagttg atagagctga gagaatagcc aatgagataa gaattgggtc
     5821 aattgaatat gcagaggatg tgctttctag tgttgagtat aatttaagag aaatattaga
     5881 agaagtaaaa agagataaag ctgaacttag acctaaaaaa taaattttaa ataaataaaa
     5941 atggagctat cacacaaaaa attccaattt atgtgtgata gctctctttt aatatagcaa
     6001 ttttttgtta tataattgtg ctttttagat tacttatatc ttattttgat attataatgt
     6061 agtttaatct tttatttgtt gaaatcaact tatttatttg tataaataat ataggttatt
     6121 ttcaaatttt tttaataatt ttaccactgt cttcttttag attagagtta gactgtgcaa
     6181 aattataaaa taatactaaa tcatgtgcaa ttatttggta tggcactaca tgtagaaaat
     6241 atagataata aaattaagga ggtagtatga aggaaagggc taaaaaaata ttagatttta
     6301 ttattaaaaa taacaatgta acatcacaag aactacaaga aaagtttaat gtttcaaaga
     6361 gaactattta ttacgatatt ctagctataa ataaacaact aggaaaaagt ggtaatataa
     6421 aaaatgtaaa acacaaattt atatttgaag gtaatttatg tgatgcaagg aaaataattt
     6481 caactgaaga agataaattt ttagatagtg attacagaaa aacttttata ttaaacaaga
     6541 tattacttgg cgaaaaaata tcaatagaaa aactaacaaa tgaaatgtta ctttcaaaaa
     6601 acacagtagt acaaacaata actgatgtaa aaaaatacct tcaaacaatg ggattaagat
     6661 tagagtataa gggaaagtac aaaattatag gtgatgaata cgttattaga gagctgtttc
     6721 tgataattgt tcaagaaaac gtactggaaa ttaattctat aagtgaggaa gtaagtagtt
     6781 tcgatacaaa aggacatata aaacttactg attattcttt attaaatcta actaagtttg
     6841 tggaatttct aaataaaagg ataagagatg ggaaaacatt atactcatat aaatatctaa
     6901 atgaagctaa aaaaattagt tactttagta attgtaaaga acttttatgt gaagaagcta
     6961 atgaaaatga acaagcctat atatgcactt acataagctc acttcccagc ttaaatagtg
     7021 aagttaagga agatgttgtt gaagaatatg tagataaact tatagataag tttgaagtaa
     7081 atacagcaat aaaattagaa agtaagcatg agtttaagaa aaatatttta aggcacctcc
     7141 actcttctta caacagaata cgatttaagt ttcctattag aaatccaatg ttagatgaga
     7201 ctaagtataa acatgagtca ctttataaaa ttataaaatc aattatagag aatgaagaag
     7261 agtttcctgt atttgagggg ataagagaag aagaaatagg atttatagca gcgtattttg
     7321 gaggatattt aagagggtct agggataatg gtctaagaag aaataaagtc ttactagttt
     7381 gtcctaatgg tcttatggta tcaaagagtt tggaaataca actatataaa tacataccaa
     7441 ccatagaaat agtaggtata gtatccatta agcaactaaa agaagtaaat gtatactacg
     7501 attacataat tacaactata gatatacaaa atgtaaacaa cgtaattgtt gtaaatcctc
     7561 ttttaacatc atcagatgtt caattactta tgaacaaact aataagtgta aaagaaaacg
     7621 agaagtattt caatcttgaa ttaataatac aagctataag aaaaaatgga gttatcaata
     7681 atgaagaagc actaaaagct gatttattaa acataataca caagatagat gaaggagaga
     7741 tgtatcaacc aatgttaaag gaattaatta atgcagaaag ggtcaatata ataaaaaatg
     7801 ttagagattg gaaagaagca ataaaaatag cctcaaagcc attgttagaa gataactcta
     7861 tagaagagtt atatatagaa aatatgataa agtcagttga gaaatatgga ccatatattg
     7921 ttttagctga tagatttgct ctaccacatg caagttctaa agaaggagta aacaagcttg
     7981 ctatgtcttt actaatagta gaagatgaag ttgatttatt aggaaaacca gtaaatatat
     8041 ttatggtatt agctgctgta gataacacta ctcacattag agcattagca tcactatcag
     8101 agatgatgta cgaggaagaa aatgtaaaac taataattaa tggtgataaa agttcaatac
     8161 tagaacttat aaataagcaa aattaggagg aatgaaaaat gaagatatta gcagtatgtg
     8221 gttttggtgt aggttcatca atggtattaa aaatgacaat acaaaaggct ctaaagcagt
     8281 taggtataga atgtgaagta gaaaatacag atataaattc agctagaggt gctgattgtg
     8341 actgtatatt cacaagtcaa gaattgttaa atgaattaaa aggaacttgt aatgtagatg
     8401 tatatccagt taaaaagtac atggatttag cagaagtcac agaagttgtt caaacttttt
     8461 taagtgataa aaatgataag aaatagtata agttttaaaa agtaaattta agttaatcag
     8521 aataaaataa aaacttacta agttcaaaaa tagtaagaaa gagaggaggg atttagtggt
     8581 agaaaactta aacagttgac ttatcactaa aaaatataat gcttgatttt atttcaaata
     8641 atattttacg taatccatct atgttaattg gattaatagc agcatttggt cttgtactac
     8701 agaagaagaa agcatctgat gttataaagg ggtcactact tgcagctttc ggaattataa
     8761 ttatggagac tggaacaggt atgttagtta gttcaatagc accaattaat ggagctttcc
     8821 aaagtataag tggtggtgca gtaacaaaag gtttaagtga tgttactttt actgcaagtt
     8881 atggtggaat tataggttta gcaatgtttg cgggtttagc tattcaccta atgatagcaa
     8941 gatttactcc tattaagaca atcttcttaa caggtcacat gctttattgg ttcccattca
     9001 tatttgtagc ttcaggagtt gaagctggat taaatggtgg agtattaata gcttttgcag
     9061 cagtattatc agcactctat tggtcaataa tgccttggtt attaagaaaa tatgtgtttg
     9121 atgttatagg agatgattct tttacattag gtcatccatc tggttgttta gcacttatat
     9181 caggatttgt tgctaaatta gtaggaaata aagaaaaatc tacagaagat ataaacatac
     9241 ctcaaagttt atcatttttt agagaaatat ctataagtgg tggaatggca gtgtttttaa
     9301 tgtttatagt tgttgggata gtaataccag gtgcgtttaa agatagtaat gagtttattg
     9361 gaacgtatgc aataaagcaa ggtctttctt ttggagcagg tcttataata atgcttcatg
     9421 gggtaagaat gctaataaat caaataatgc cagcatttca aggtatatct gaaaaagttg
     9481 tgccaaatgc actacctgct tttgactgtc caatattatt taattataaa ccaaatgctg
     9541 tattaatagg attcttagtt gctatgctaa catctacagc tatgatttta atatgtaact
     9601 actttaatgt atttaatttg ctacttatac cacttgttat aacttcattc tttgagtgtg
     9661 gagcagcagc agtaatagga gaatcacaag gtggtatacg tggagctata ataggtacag
     9721 ctatagctgc aatatttatg gtagttttag taggaatatc tgctgtaatt tactcaggaa
     9781 ctattcaaaa ttggatacta atatttggag gtaatgacct ttcattattt ggtataatca
     9841 gtaggtttat aggttcacta tttggaggaa tattatagat atgaaattta aatacataga
     9901 tgagataaga agattaattg atataataga aaacgaagaa aaagtcaata tggaaaaagc
     9961 agtacaaatc cttgttgaag caatagaaag taaaaatgct atattttcat ttggagcttc
    10021 acatgcagga atattaacag aagaattatt ctatagagca ggtggctgtg ttcttataaa
    10081 tccagtattt gcaaaatctc taatgttaga tacagcacct ataactcaca caagtaagat
    10141 ggaaagatta gaaggatatg gtacagctct agctgataaa actccaatta aaaaaggaga
    10201 tgtagttata gttcattcag tatctggtag aaatccagta agtatagagt ttgctatgaa
    10261 agctaaggaa aataaggcaa tagtaatatg tataactaat ttaagttact caaaaagtgt
    10321 atcttcaaga cattcttcag gaaaaaaact ttatgaagta tgcgatttag tgatagataa
    10381 tcatggtcaa aagggagatg cctgtgtaga gatagatgga ttaaaccaaa aagttagtcc
    10441 aacttctact gtaatagcaa ctactgtgat gaactctatt atagctcaag ctactcagga
    10501 attagttaat aaagggctaa agaatcctcc gattttctat agtgccaata ttgatggtgg
    10561 agatgagtta aataagaaga tttttgatga atataaaagt gtaattcatt atgaatatta
    10621 aggttaaaaa aatatgttaa tgaactttga agtttatcaa caaagaatat taataatata
    10681 taataaatgc tatcatttaa aagctgtagg taaaaatcta cagcttttta ttattgtaga
    10741 ttaaaaagat gcgtaattaa attttaaatt ggagaaaata aattggaagg tatcaattat
    10801 aaatattaga aacgttgtca tagaaaaaaa ctatattaaa aaaaagtaca tagaaaaaac
    10861 tgattagaca ataaatacat gctttgataa tattttaaat ggagtgaaaa tgtgtagaat
    10921 tataatgaaa aaagaaaata aaaatgaaaa aagaaaataa aatggaggga ataaaagtgg
    10981 gtatgttaaa aaaattatgt tcactagcta tggtagcaat tatgactaca acacttataa
    11041 cagggtgttc aagtggaaat ggaaaagatt caaaaaagga tggcgaaaaa gaaaaactag
    11101 ttctttggat gccaccagta gaaaataata tgaaggaagt ttgggaccct attttagata
    11161 aatttgagga aaaaaataac tgtgaagtag atttacagat aattccttgg gataattatt
    11221 ctgaaaaatt tgcaacagct ataagtgctg gcgaaggtcc agatgtagga tatatgtatg
    11281 cagaaatgta tccacagttt atagaaagtg gagcagtaga agatttgact aactatgcaa
    11341 ctaaagagga taaagagcaa tctatttata taaaaacatc tgaaatgatg ggtggaatgt
    11401 atggtatgcc attccaagca gcaaatccag gggtattata ctataacaag gatattttag
    11461 agaaattagg agaaaaacct cctaaaacat gggaagattt taaaagaatt tgtaaaaaag
    11521 caacgcaaga tactgatggt gatggaaaga tagaccaatg gggattagct cagggatggg
    11581 gagctaaaac ttttggtaac ttaaactgga actggtatcc ttatttatgg caagctggtg
    11641 gagatatatt taatgatgat ttaaaatcgg ttaaatttaa tgataagagt ggtttagaag
    11701 cagctaagtt cttaaaagaa ttacaagcat atgtgcctga agattcttta tctaaagata
    11761 gtaatgaaat gatagaaagt gtatttggac cagggaaagc agcatttaca ataatgttat
    11821 cttctgctgc aacaagtgta tttgatgaat cattcccgga tttaaattgg ggatttgtaa
    11881 ctggactaga agataagaaa gcagcaactt ttggagcagt agaccattta tctttaatgt
    11941 catctgcgaa aaataaagaa cttgcataca agttaataca acatatgtta agtgttgaat
    12001 caatgacaga atttcataaa gcaataccaa gagcaccaat aacaaaaggt gaaccatatc
    12061 aaggtgatga aagattcaaa gaaatggtgg aaaatgacaa aaatgtatat agaccattag
    12121 tagtaggacc tcatggagtt gaaatatatg aatatttatg gaaagaactt caaactatga
    12181 tttcaggaga taaaactcca aaacaagcgt tagatgatgc tgcaaaatat tcaaatgatt
    12241 tattagcaca ataaaagttt aaagctgtaa tatgttgtaa aggactttac agattaaaat
    12301 tagcattgta tttataaata caatgctaat atttatattg gagaggataa aatggaaaac
    12361 ttagttagag agtttgaaga aaattcaaaa agaaaaaaaa caaaattaaa tattaaacca
    12421 tatatgtata tattgccatt gggaataatt ttagtggcgt tttatgtgat accaataata
    12481 atgtcaatat actttagttt tacaaagtat aatattatta gtccagctac ttttataggt
    12541 ttagaaaatt ataaaaaatt atttacagat gaaacattaa aagtatctat tataaatact
    12601 attaaattta ctgttgttgt agtaccttgc caaactatat tatcattgat tttggctgtt
    12661 tggattacag gaaaaggaaa tagtaaaata gcttcatttg ctaaaggtgc tatatttata
    12721 ccagtattat catctatggt tttgataggt atggtatgga gggctttatt gaatggagaa
    12781 ggttctatta tatatcaggt attaggtgcc tttggaatag aatcatctaa acttttagga
    12841 gatagtaaga cagcattacc tacacttatg tttatatcta tgtggaaaaa tatagggtat
    12901 ttcatggtaa tttatatttc agctataatg aatttaccaa aacactgcta tgaagtagcg
    12961 aaggttgatg gggctacaaa acttcaagaa tttataaaaa taactgtacc tttattaaaa
    13021 ccaactacaa taatggttgt atttttaggg tcgatatggt cattacaagt atttgactta
    13081 atttatactg tgacaggtgg aggtccaggt atatctacta tgagtatagt tatgcatgca
    13141 tttaacttaa actttaaaaa ctttaattct ggatatgcaa tgactgtagc aaacgtatta
    13201 tttttgttaa tagcagtagt ttcgatttta caaaataagc ttgtaagacg agacaattca
    13261 gatttttaga aaggtaggta taatatgaaa acagcaagaa gattattaag tgatggttta
    13321 cttttattta tagcgtgtgc aagtttggta ccttttatat acatgttgat aatatcattg
    13381 aagataacat ataactcata tagtttggat atatcttttt caacagtaac ccttcaaaac
    13441 tatatagata tttttactaa aaaaggattt gcacaatatt tttttaatac agcaatagta
    13501 tctttttcag gtgtattatt aaatttagta tttagtacat tagcaggata ttcttttgcg
    13561 aagatggatt ttaaaggtag tgataagtta tttttattta tgataatgac tttaattata
    13621 ccttcccaag ttacaatgat accactatat ataataatga aacacttagg atggataaat
    13681 agttatcttg cacttattat gccaatacca actgcatttg gcgtatttat aatgagacaa
    13741 gctattttag gagtaccaaa agagttatta gaatcagcaa agatagatgg ttgctcggat
    13801 tttagaattt taatacaaat agtgttgcct ttaattaaac cagcattaat tacactagca
    13861 atatttactt ttatgggagc atggaacgag tttatgtggc ctcttatagc tacaactaaa
    13921 gatgcaatga gaacattaac agttggatta tctactctta agacattcca gataactaat
    13981 tatggacaga tgatggcagg ggctacaata acatttttac cgccatttat tttctatttg
    14041 attttgcaga gtaaatttgt tgaaggtgtg tctttgtctg gtataaaagg ttaaatagtc
    14101 taatttaata atatataaag aaacttatat aaatatggag gaatttaaac tatggaaatt
    14161 tcatatgtac tgaaagattg cacattcaaa cacaataaat attcagttat agaagctgaa
    14221 acttggaaag ataaggattt ggatattgta gtagctaaag gtgaaaaatt tgcatttcaa
    14281 attatgttaa aagcaacaga agaatttaat tgtacaattg ataaaagcag tgctatatca
    14341 tggaaaggcc taggaaatag agtaaggtta gcattaaatg tgcctaatag tttagaaaat
    14401 aattttagta taaatatatt aggatatgtt caagatgata caaaggcatt tgtgaatgat
    14461 gctattttaa gggataaaga tgtgttagta gagcaatatt tgcctcaaac attttggatt
    14521 gaaggtcaag taccagaaga ttttgaagaa aataaccttg atataggtat tgatatattt
    14581 aaaagctttg gatatgaaga cgaagaaaaa gtttgtacaa ttgatgttcc tgtaaaagta
    14641 agaaatgttg tgctaaagcc tttagatgaa agcaagttct ttttagattt atggcaacat
    14701 ccaagttgtt tagctagaat gtataaagtt gagttatggt ctgatataca ttttgaaata
    14761 gtcgataact atttaaaaga attagcatct ttaggagaaa aagttgcaac agttatagtt
    14821 tcagattatc catgggcagg gcaaagttgt tataaagttt ataaaaatcc atcaaactta
    14881 tatgaatata acatggtaag tgtatcaaaa ggattggatg gaaagattaa atgtaacttt
    14941 gagagtatgg ataggtatat aggcatagct gataagtata aaatggctaa ggagatagat
    15001 ttatttggtc tattaggtaa ttggtgtgct ggtgagtttg gaaatcctgt agaaggttat
    15061 aaggacccta taagagtaag atattttgat gaaaaggata aagtatttaa atttataaac
    15121 aatacaaatg atttaaaaga atatatagga ttagtattaa atcatttaat agagtgtgga
    15181 ttatgggaca gagtaaggat tatagctgat gaaccaaata atccagaagt agtcaaagag
    15241 tgtatagaat ttataaattc tactgtaggt actcatcaag ttaaatataa atcagcaact
    15301 cacgaccaaa actttttaga cagagctaaa gatgaaattg atgatatgtc aataaattta
    15361 aaacttacta tacaaaatta taaagatata gaaagtctta agaaaaaaat caatgacaag
    15421 ggtgggatat tgacttggtt tgtatgctgt tttccagaga aaccaaatag ttttttaagt
    15481 tctccttttg tagaaaatag aataatagga tggtatactt attactttgg tcttgatgga
    15541 tttttaagat gggactataa tttatggaca gaagaccctt ggaaagattc aagttacaaa
    15601 ttccctattt ggaaagctgg agatatgttc tttgtttatc ctggaaaaga tttaaagcca
    15661 gttagaagtg tcagaatgga gaatttaaga tttggaatac aggactttga gttatttaca
    15721 atgttagaaa aagaaaaggg aagagagtat atagaagttg agcttatgca agaattatta
    15781 aataaaaaag aaaatgctga aattaaaggt tttggtgaca tagaactagg atattcactt
    15841 gataattggg gatatgataa agttaaaaaa tatgtattag agttgttaga cagagtataa
    15901 aaaatataag agtgtctcaa aatgaacttt ttagttcatg aggcactctt tttgtatgaa
    15961 atcaatatag tttcattgtt tcaacaatga aaaataggat tatttctatt ctaagacagc
    16021 cctttagttt tttagttgta ttcaatgttt taaaactaac tataaacgct ataaagttta
    16081 taaatgacaa taattatata taacatgatg tactttaatt tgctataata gggattgaat
    16141 aatattatat ggaagataac attgtttggg gggattaata taaaaagaat aggtattatt
    16201 ataatgtttt ttttaagtat atcttttatt ggattttatc ttgtaataag agataacagt
    16261 gatacagtta agatgcaacg agggataaat ataggtaatg cactagaatc acctaaggat
    16321 tttccgtggg atgtaaaaat gtcaaataag ttttttgatg atataaaaga tgcaggtttt
    16381 gacacagtac gtataccagt aaggttttct gattatacgt cagattctga taattttaaa
    16441 attgatgaag aattttttaa aaagattgat aaatatgtag attatgcatt ggataaagac
    16501 ttaatagttg tgttggattt acaccatttt gaagaaatta tgaaagaacc aagagttcat
    16561 aaagaaaagt ttttaaaaat atggcaacag atagctaata ggtatcaaaa atatgataaa
    16621 aaattggtat ttgaattatt aaatgaacct aaagaaaatt tatattcaca gttactaaac
    16681 gaatacattg aagaagcaat aaagataatt agaaaaacta accctaaaag aactattata
    16741 gtaggaccat ataatttcta tcaaattgat tatttgaatg aattaaatat tcctaaagat
    16801 tctaatatag tagtttcctt tcactattac gagccaaatg attttgcatt tcaaggaaat
    16861 atctatcata agggatttga gcacttaagc aatattactt gggaaggaac aaatgaacaa
    16921 atggattatt taaaaaaaag gtttgatact gtggaaaatt gggcaaataa aaataaggta
    16981 aaaatcttct tgggtgaatt tggagtaaca aaagaagcac cagaaacttc aaggagagct
    17041 tgggtaaaag ctgtaagaga agaagctgaa aaacgaaact tttcatgggc ttattgggag
    17101 cttgcttctg gatttggtat atacaatcaa attgaaggga cttgggatag agaaatatta
    17161 agtgcattaa tagaaaagag gtgattttat gaagaaaaat tcaaaaaaga tacttataac
    17221 tttaactatt attactaata tagtatatat attgtggaga attttttata cagtacctaa
    17281 agaagaaggg atgtttgccc taatctgtgc cataatactt ctttttgtag agataatggg
    17341 tatgatggaa atgtttgtgc attattatgg tatgtcaaat attgagtacc ctgaaaaacc
    17401 aatcataagt gaagaacttt atccacatgt agatgtattc attgcaacat ataatgaatc
    17461 tgttgatttg gtgagaaaaa ctgtaaatgg gtgtattcat atgcagtatc cagataagaa
    17521 aaaggtacat atatacatat gtgatgatgg aaatcgtgaa gaaatgtgta ttctagcaga
    17581 aaaaatgggt gtaaattaca taactagaac tgaaagagaa ggagcaaaag ctggaaattt
    17641 aaataatgca atgcaacata caaattcccc tttgattgct acatttgatg cagatatgat
    17701 accaatgcat gactttttaa ttgctacagt accatacttt ttgaaaaatg agcaagctaa
    17761 aaaagatgga gaaaaagagg agtatgaaaa ggttggtttt gtgcaaactc cacagagttt
    17821 ttataatcca gacttgtttc aatttaatct gcactctgaa ggaaggattc caaatgagca
    17881 agattatttt tacagagaca ttcaattggc tagaaatagg acaaattctg taatttatgg
    17941 tggtagtaat acagttattt caagggaagc acttgaagaa gttgatggat tttatactta
    18001 ttctattaca gaagattttg caacaggtat cttaattcaa agtaaggggt atagatgtta
    18061 tgctattcca gaagtacatg catcaggttt atctccgaca gatttaaaaa gtcttattaa
    18121 gcaacgtgaa agatgggcac gtggatgtat acaaactggt agacgtctaa atattttgtt
    18181 taggagaggt ttaggttttt ggcagaaaat aagttatatt tcttcaatta cttattggta
    18241 tgcaagtata aaacgctttg tatatataat ggcacctatt ttattttctg tgtttaatgt
    18301 gattgtagta aaatgtacgt tacttcaagt attggtattt tggttgccaa tgtatatttt
    18361 aagtagttta tctcttaaaa tattttctca aaatataaga aatactagat ggaccaatat
    18421 atatgaaact ataatgtttc agtctttaat gccagcagta atacttgaaa cgtttgcaat
    18481 atctaaaaat aaattttcag taacaaataa gagtaaatta gaagaaaata gaatgtataa
    18541 gtttttacag ggaatacctt attttattta catggtctta tctatcatag gaattttaaa
    18601 aatgtttgta gcaatattta aaatgagttc aatgacatat tcagttgtat tattttggtt
    18661 gattggaaac ctttttaacc ttgttatggc tacattattt atatcaggga gacagcagtt
    18721 aagaaagtct gagcgttata ttgctgaaat tgattttaag ttaaagcaaa attcttatgt
    18781 tctttcttca aaaacaattg atatatcaga aaatgggttt gcatttttgc tagaaaatcc
    18841 agaatatatt tccccagaag aagagtttga ggtagagttt agagaaaaat ctggaaatga
    18901 aatgtatata gctaatatga aagcaaagat tgttaatgtt gtggaagtaa attcaaagtg
    18961 gaaatatgca gcttatatta cacatattga ggatagtgaa atagataatt ggatgtgtat
    19021 tgttcacgat agaataccta ctttaccaat gacaatatct aatcaactag gtttctttga
    19081 tgatttgcaa attaatgtaa aaaaacgtat tgaaaaaact agaacattat cacgaagaag
    19141 tccaagaatc aatatgaatt ttcagatgga tataaaaaat acaggtaaat taagaatagt
    19201 gaattttaat tatcaatatg tattattaaa ttttgagaat aaaagtattt atcctaaaga
    19261 aatagcattg gaaattaatg aaaatattgt gttagaatgt gatttgtgtg agggtaaaat
    19321 tgatgaaaga ggtatcttat ataaagtaaa taacattgac agcataatgc aaaatttctt
    19381 tttaagagat gaaatgatgg attggatatt acaaaataag acaattcttg tttcaaagcc
    19441 tagtgaaaag aaggaaaaaa gtatagatga gtttgaaccc atggaatata tttaggagag
    19501 atgaaatgaa gactttaaaa gtgttttttt gttctacaaa atatcttatt ttggtgttta
    19561 ttttttttat aaatactact tttataagtt cagcaacaag tgataaaatt ttagatttat
    19621 cctttaaaaa gatagagact gacttaagtt ctaagattac atatgaggat acaggtgtaa
    19681 gaatagaaac ggatagttct aaaagcgata aggaacgtta tttatatatt taccaaaata
    19741 taaaagaaaa ttggagcatg tataataatt tttatattga aattcaaaat aaaaataaaa
    19801 gttctcagag aataaattta agcatacaaa gtaaaaatat gcttgaattt agattaaaag
    19861 aaggtagtga agtattttta gaaggtaaaa atataatata ttcagataaa ataaaagaag
    19921 gatgcataga agtaccaggt gaatttgaag gcaaaattta tgtgaacttt aatagcctta
    19981 taaatgagga aagtaatgtt gtgctggact caaatatgct ttcaaacatt gttagttggg
    20041 gaattacgtt tattccatct gatgaagaac ataatattgt tattataaaa aagatatctt
    20101 tattgtctga agagaaatta agatttttaa ataatattaa aataattggc gatgaagaag
    20161 tacaaatacc agtattgggt caaagtataa gtcagtatga agtattaggt cttaaaagtg
    20221 attctaaaat taagtactct cttatgggaa aacaagataa tgtgagcata tctcaaaaag
    20281 gtaaattaac attaagtaat aaagccaaac ctggacaaat tattttacaa gttaatgttg
    20341 atgataagtt taaaattgga aagaaaataa cattaactga gtcttggagt gttaataaaa
    20401 aagataagga tggtgttcca tatacattag tatatcctga gcaatcacca acagtacaag
    20461 atatgaaaaa aataaacttt atgaataaca tcataacgtt tgttcgaatt ttatttgtat
    20521 ctctagtaat aatatgcttt ggaatatatt tatattggaa aaaatgttca aaaactaagt
    20581 aaagagagga ggttagattg tgttattttc aagtttaatt tttttatttt attttttacc
    20641 tataactttg gttttatatt atgtatttag gtttaaccga acgatacaga acatgatatt
    20701 gcttgctgtt agtttatttt tttatgcttg gggagagcca aaatttgttg ttatcatgat
    20761 agtttccata ataatgaact atatatttgg tttactggta gatagatata gggaaagtaa
    20821 aataaaagta aaagtttttt tagttttgat gtgtgcatac aatataggtg ttttatttat
    20881 atttaaatat ctagcttttg cgctaagaaa tattagtgaa cttattagta ctgaattgac
    20941 tatacctagt attgttttac ccataggaat ttcatttttt acatttcagg gaatgtctta
    21001 tgttatagat gtatatcgta gacatggaga agtccaaaag aacccttttt atgtgggatt
    21061 gtatatagca tttttcccac aattaatagc aggtcctata gttagatatg agagtgtggc
    21121 agaacaaatt ctcaatagaa aagaaacttg gaataaattt tctattggta cttgtaggtt
    21181 tataacaggt ctaggtaaaa aagtattaat ttcaaataac atggctatag tagcagacta
    21241 tatttacact atgaatagtc aaggagaaat agctgcatca cttgcatggc ttggttcaat
    21301 agcctatact ctgcaaatat tctttgattt ttctgcttat tcagatatgg ctataggctt
    21361 aggtcttatg tttggtttta aatttgaaga gaactttaat tatccatata tatcaaaatc
    21421 aattagtgag ttttggagaa gatggcacat ttctttggga atgtggttta agagctatat
    21481 atactttccg cttggaggtt ctagagtagc aaataaagat attatgatta gaaatatgtt
    21541 tatagtgtgg ttattcactg gaatatggca tggagcagaa tggacatttg taatttgggg
    21601 gattttaaac tttgttttcc taattataga gcgatttata atgtttgaaa agattgaaaa
    21661 tcacaatttt ataaaacata tttatgcttt attagtagtt aattttggat gggtattgtt
    21721 taggtcacct aatttaaaag aagcatataa ttattttaaa gctatgtttg gaggcaatgg
    21781 agctatatgg agtgattata catatatgtt tttaaaagaa tactgggttt tctttatatt
    21841 tgcatttatt tttagtgtac ctattgcaaa gaaaatcaat aagtttgttg ttgaacaagc
    21901 taaatatagt atgatattta acgtatttta tccaatctca gctattttat tgttttttat
    21961 ttctgtgaca tatttagtaa ctggaagcta taatccattt atttatttta atttttagtt
    22021 gggaggaaaa gtatgaatag attttttaaa gtaagattaa taactgcaat tatgttctta
    22081 attattttat tcgctttctc tatctttaat gtttgttatt ctggaaaaga tacgatacag
    22141 gaaataaaaa attcaatagc tgaaaaaagt agtttaaaag atacaatatc atctattgat
    22201 aatacagtga atgacaaagt aatatacaaa aatacgttca ttgaaactta tggctattta
    22261 caaaaattaa tgggtaaaaa tgagttttca aaatttagtg ttgtaaaaga tacttctgga
    22321 aagatgcatt atacatattt tgctaaagct ccaaatccag ttggtgtgat agcagatagg
    22381 gtaagaaaat tttctgatga aatggaaaag caaaacacta aattggttta cctaatgact
    22441 ccagataagt atataaaagg agtgacaaaa tttcccaaag gaatacctta ttcttatagt
    22501 aatgagacag cagataattt tttaaatcaa ctaaaaaagt ataatgtgga ctatattgat
    22561 tttagagaaa atattttaaa gagtggtatt ccaaaagatg atttattttt taaaacagac
    22621 catcactgga aagtagaaac ttctttttgg gcatttggag aattagttga gcaacttaat
    22681 aaaaaataca atatgaattt agatgaaaat cattattata gagataagga caattataat
    22741 tcaattgtat atcctaagtc atttcttggt tctatgggaa gaaagactgg tatcttatat
    22801 ggtggtatag atgattttac attaatttat cctaaattta agactaatta tacttattat
    22861 acagattcta aatctcaaaa atttgaatta aaaggaaggt ttgaagagag tttaatatta
    22921 tcatatcctt ttaatgcaga ccttaattta atggatggtc aatcagataa gtattttaca
    22981 tatttattgg ggaacagacc attagttaaa ataaaaaata tagaaaatcc agatggatta
    23041 aaagtgctgt ttgttaaaga ttctctaatt gtaccaacag cagcattttt ttcctctgtt
    23101 tgttcatcta tagatatgat agaccctaga tattatgatg gagatatact agaatatgca
    23161 aaatcccatg actatgactt tgtttttata tctgtatatc cccaaaatct tacaaaagaa
    23221 ttttttccgt tttgtgagta aatttctaaa aaagttttct gataaaataa atggctaact
    23281 ggtaaaaagt tagccattta ttttgagtgt tattctaaga aatatttttt ttactaaata
    23341 cttctttatc tatttttcca agtgattttc ctacagtaat tgatgatacc atagcaccat
    23401 taacatttat catagtacgt cccatatcta gtatagggtc tattgcaagt ataccaccaa
    23461 ctaatggaaa ataagaacca agaccaactc cagagataac aactgaaacg gccattgtag
    23521 cagttcctgg aagaccagct attccaaacg aactaattgt tataacaatt aacaacataa
    23581 cataaaagct catatccatg tttactccag ccatattggc aagtgttaca gccattaaag
    23641 caggatatat acctgcacaa ccattcatac ccatatttgc acctaaactt gctgtaaagc
    23701 ttgatacacc ctcatcaact ccgtgatttt gtactagact ttctatcgtt acaggcaatg
    23761 ttcctaaact agaacgtgaa gtaaatgcca gaagtaaagc ttcagttgaa ttttttatat
    23821 aagtaatggg atttagacca tttaaagtta taaaaattaa atgtatgata aataatattg
    23881 caactgatat ataaagagct aatataaact gtaaaacaga tactattgaa ctaatacctt
    23941 gtgaagttat tgtatttgca agcagagcaa ccacagcata aggcataaat ttaataacag
    24001 tcatagacac acttacaatt attttgtaag atgcttctat aaaatctata aatggtttta
    24061 ttgtctcaaa atgtttttta cttaatctct ttattgaggt agctataaat gtagcaaata
    24121 ttattgtagc tacaatattt ccatttgcca tagccattac aggattagat ggtagtaatc
    24181 ctcttaaagt atccacaaga gagtttattt ctttaagttc ttgattttca actaaagtag
    24241 gcatattagc tcctacacct aacttcatta tatttgcaac gataattcca acaattgcag
    24301 ctatagcagt agtagcaaga agcataccta aagatttaaa tgtcagagtt ccaagattgt
    24361 tatctttcat atttataatt acttttataa tggataagaa aactaaaggt actactaaca
    24421 tttttaataa gtccataaat ccagaaccaa ataggctata ccacttactt acttcttgta
    24481 gccaaatgac ttgagttgta tcattaggga aaccagcaaa agcttgtatt atgattccca
    24541 atatgagtcc tagtactgta gcatatattg ttctcaatga aaattttatt tttttctttt
    24601 caaattgtct aacaataaag aacgtaccta gaagtaatat tatgaaaaaa attgttttaa
    24661 aatctgtaat cataagaaat tgtgataaaa atgaactttc caatattttt ctccttttat
    24721 ttaaaaataa atttttctaa gtttaaatga tatcaatata ttaaaaaaat gtcaatatta
    24781 tatcacaatt tacttaaata gagtaaaaca agttgatttt gaaaaatgtg attaagtaca
    24841 gaaaaatagg gtataataaa tgtttaaaga atttgaattg tggtaagaaa ataaaaaata
    24901 gcttttataa gatttgattt caaattaaaa tgtgttaaat aaataactaa aaattattat
    24961 aaaaaatgtt ttcatagaat atatatcaaa tgaaaagctt aaaataagtg aagaaattaa
    25021 ctagacatca aaggagagat aaaatgagag taataataat tggtggtgtt gcagctggta
    25081 tgagtgctgc agcaaaatta aaaagaataa aacctgagta tgaagttgtt gtttatgaaa
    25141 aaactgaaat agtatctttt ggtgcttgtg gattaccata ctttgttggt ggatttttta
    25201 atgatgctga tgaattatta gcaagaacac cagagaaact tagagaagct ggaattgatt
    25261 taaatatatt tagagaagtc gttgaggttg atagtgaatc taagaaaata aaagtaaaaa
    25321 acataaaaac agatgaaata tatgaagatt actatgataa attgatgata gcaactggag
    25381 caagaagtat tatgccttct ataaaaaaca taaaattgaa gaatgtttct acattaaaaa
    25441 gtttatatga tggagaatat ctaaaaaaac ttttaagtaa tgaagataat aaaagagtga
    25501 caataatagg agctggtttt atagggttag aagcagttga ggcatgtaag aaattaggta
    25561 aagatgtaga tgtaattcag ttagaagata gaatattgcc acaagtattt gataaggaga
    25621 tgacggatgt attagaagaa gaaataaata gacataatgt taatttgcac ttagatgaaa
    25681 tggttgtaga actttgtgga gaagataaag tagaaaaagt tataacaaac aaaggggaaa
    25741 tagatacaga tgtagtaata attgctactg gcgtgagacc taataccgag tttttgtcaa
    25801 atactaatat aaaaatgcta aaaaatggag ctatagtagt tgatgagtat ggaagaactt
    25861 ctgtagaaga tatatactct gctggagatt gtgcaactat aaagaatata gtaagtaatg
    25921 aaaatgtata tgttccttta gctactggag ctaataaatt gggtaggata gtaggagaaa
    25981 acctagcagg aagagaagta tcttatcaag gttcactatc ttcaagctgt ataaaaatta
    26041 tggatatgga ggcagcatca actggaatta cagaaaggca agctaaagat ttaggcataa
    26101 atgtcaaatc taaatttatt tctgattata atcaaactca ttattatcca ggaagaaata
    26161 aaatatatgt aaagcttatt tatgatgcag atacaaaagt tattttaggc ggacaagtgg
    26221 ctgggtttaa ggatgctgtc caaagagcaa atgtattggc agctgctatc tttggaaaaa
    26281 tgaccacaag tcaacttgga atgttagatt tatgttatgc tccaccattt gcaagaacat
    26341 gggatgtttt aaatgtagct ggaaatatat ctaaataata tttagattat agctaatata
    26401 gttgacaagc aattaaggga atgaatttac ctaaactatt tttatcatgg taaaattcat
    26461 tcctaaattt ttattccaaa tttttattcc aaatttttag aaataaggat tccattaaat
    26521 tattcatcaa aatttaaacg caagcttaat ctatcttctc caagaatagt attttcaaaa
    26581 acattagtag cattttctaa atacatacta ggtatgtcta aagaaggact aaaatgagtc
    26641 agtaaaagtt gtttgacatt tccaagtttt gcaagattag cagcttctct aaaagtcata
    26701 tgtttgtttc taacagcttt tgatatatct aaatcatcac catacatggc ttcacatata
    26761 aataaatcac tatcttttat gaaatctggt attgattcta taggtcttgt atcggttgta
    26821 agacttatct taactccctt tcttaattct cctaagacca tatctggagt gtattgtttt
    26881 gaatctaaaa ctatattttg accttcttgg agtttgttcc ataatatttt aggaacttcg
    26941 tttttagttg ccttatctat atcaaattta gggtttcttc taaaattaaa actgtatcct
    27001 aaacactcta tagagtgttc taaactgatt gttgaaattt ctaaatttct taaaacttta
    27061 ttatctaaag aaaaattgcc ttgagggttt tctataattt ttaaagtgta tggtacatat
    27121 tctactaaat ttctcataga acgtatacaa tcgactattc caactggacc aactatagtt
    27181 aaatcacttg tacgaccact attcccaatt gttgataaaa gtcctaaaag tccaaatata
    27241 tggtcaccat gtaaatgggt tatacaaatc aagtctatat ctttaaagcc acattttttt
    27301 agcttcatag atacctgagt accttcacca cagtcaatta gtatttttct acctttatag
    27361 tttataaata cagaggatag aaatctgttt ggcataggta catttccacc acatccaagt
    27421 aaagttaaat ctatcatttt ttcccctttc taaaattaac acaaatctgt tgtttgtttt
    27481 tattatgata tcctattata attattttaa cattaaatat aacaataaat gtgtatttga
    27541 gtcaagaaat attgtcaaat aaagttttta tataacaatg tataaaaatt tggaggacaa
    27601 aaagtgaaaa ttatatttag aagtatttaa aggaggaaaa tttaatgaaa gtattaaaga
    27661 aaattactat tttgttattg attacattaa ctttagttgg ttgtagtaac ggagaagaga
    27721 aaaaagtggt tgaaggttct aagaaaactc aaaattctaa aaaagatgaa cagcctataa
    27781 tagtgcataa agatgcaaat atattgaaag ataggtatga gccaccaaaa ggatttaaaa
    27841 gagttgatgt agaaaaaggt agttttgaat catttcttag gaatactaag ctaaaaaatt
    27901 atggagagaa agtaaagtat tatgatggaa gaactaagga atcaaaaggt atttatgata
    27961 gtgtatttga tgttgatatt ggaaatagag atttacatca atgtgcagat gcaattatgc
    28021 ttatgagagc agaatacttg tatcagaata aactatatga tgaaataagt tttgattttg
    28081 ttgatggttt taaggcgaag tattcaaaat gggcacaagg atatagaatt tcagtaaaag
    28141 atagtggttc aagctggtat aaagcaactg aatattcaac atcttatgag agtttcagaa
    28201 agtttatgga tatagtgttt gcatattcag gaacgttatc tcttgaaaaa gaattggagc
    28261 ctgtaaaagt tgaggatatg aaaataggag atgtgtttat agttggtgga agtccaggac
    28321 atgcaagtat aattatggat atggcagaaa atgaaaagac tggtgaaaag gtatttatga
    28381 tagcccaaag ctatatgcct gcacaacaaa cgcaacttct tattaatcgt aaaaatccaa
    28441 atttgagtcc ttggtatagt ttagattttg aaggtgattt gacaatacct caatggactt
    28501 ttaaaagaga ccagttgaaa agattttaaa ataataattt tatataataa aagaaaaata
    28561 tagatgtgaa aaaatgtaat tagattaaaa ctaattggat atggacaaaa ttaaattgat
    28621 agataaatat taaaaattac actaattaat cttactatag tttaatattt atctaaaaag
    28681 ttttatgtaa aatataaatt ttatattaaa gctaaaacta atttgaggag gctaatttat
    28741 attgaagatt agaaggtatt taattttggt aattataatt ctaattacaa caagtcagag
    28801 ttttatttct tatgggctag agcgatttga agaaataacc atttttcaca ctaatgatat
    28861 acatggaaga tatgctgaag gtgatgacca tatacaaata ggaaatctag ccacattgaa
    28921 aaaagaaact ccaaactcaa ttttagtaga tgcaggagat tgtttgcatg gacttccaat
    28981 agtaaatatg gatagaggga aaagtgcaat tgaattaatt aaatgtgctg gatatgacta
    29041 tattactcct ggaaatcatg actttaatta tggaaaagat agactgtttg agttatctaa
    29101 aagtgctaat ttaggagaga atactttaaa atttttatca tcaaatattt ttgaaaatgg
    29161 aaaaaaagtt tttgatagta atgatataaa agaaatagat ggaataaaaa taggattttt
    29221 tggactctca acccaggaaa caaagagcaa aactggattt aaaaatgtgg aaggattaga
    29281 gtttaaaaat cctatagaaa gtgcaacaga acaagtaaat gaattaaaag gaaagggtgc
    29341 caatgtaata gttgcaatat gtcatattgg tacaaatagt ttaagtaagc caaatagtat
    29401 agatatagca aatgaggttc aaggaattga tttgattatt gatggacaca gccacactaa
    29461 gtttgaaaat ggtaaacaag tgggtgatac attgattgta agtgcaggac aatatttaga
    29521 aaatattggc gagataaaac taactttgga tactaatgat gtagaaaata taaaaataga
    29581 aaataaatca gttagattaa ttggaaaaga agaagcatta aagtataagc ttgactccgc
    29641 tgtaagtaaa aaggttgatg aaataaaaga acaacaagaa aaaatattag ataaagttat
    29701 aggggtaact acaaatactc tagatggttc atatgaaaat gtaaggacaa aagaaactaa
    29761 tttgggtaat ttaataagtg acattttact agataaaact aaggctgata tttctttatt
    29821 taatggaggg aatattcgag atacgataga aaaaggcaat ataacaagaa gagatattgt
    29881 agatgtattt ccttttagca atactatagt taccaaagaa cttacaggag cacagattaa
    29941 agatgtattg gagcatggag taaaacttta tccagaaaaa agttcagcct ttttgcaagt
    30001 gggaggaatt tcatattact ttaatcctaa acaaaaagaa ggcgaaaaaa taacaaatat
    30061 tcaaaaagaa ggaaagccat tagatttaaa taaaaagtat gtagttgcaa cgaatgatta
    30121 tatagcatca ggtggagatg aatttccttg tttttcaaaa gagccagtac taaaggaatt
    30181 tggaaattta gagagtgcag tcattgaata tatagagtac aaaaaagaaa tatcaaaaaa
    30241 tgtagatgga agaattggta ttaaatctaa agatgagtta ataagcaaga atcatgagag
    30301 tgatttgata ttaagtgaaa atgaaaaaaa tatcaatgat aatgaagaga atctatctaa
    30361 taatataaaa agctctgata gtgataaaaa taaaagctta gataaaacaa gtgctagaga
    30421 aagttctaat ctaggaatat ctaaaagaga agctaaagaa aaatctccta aaacaggaga
    30481 tttaggtttt t
//
LOCUS       DAKBNN010000020        29968 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000020 DAKBNN010000000
VERSION     DAKBNN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.020"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..533)
                     /gene="pilM"
                     /locus_tag="OUB02_000904"
     CDS             complement(<1..533)
                     /gene="pilM"
                     /locus_tag="OUB02_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HCT8884680.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINL"
     gene            complement(586..945)
                     /locus_tag="OUB02_000905"
     CDS             complement(586..945)
                     /locus_tag="OUB02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCT8884681.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(999..2084)
                     /locus_tag="OUB02_000906"
     CDS             complement(999..2084)
                     /locus_tag="OUB02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCT8884682.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISIVVTNNLAYIIFSFILFSIGNIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVVDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(2255..3634)
                     /locus_tag="OUB02_000907"
     CDS             complement(2255..3634)
                     /locus_tag="OUB02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCT8884683.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3627..4307)
                     /locus_tag="OUB02_000908"
     CDS             complement(3627..4307)
                     /locus_tag="OUB02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HCT8884684.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYNNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4698..5777)
                     /locus_tag="OUB02_000909"
     CDS             complement(4698..5777)
                     /locus_tag="OUB02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8884685.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6065..6180
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6355..7725
                     /locus_tag="OUB02_000910"
     CDS             6355..7725
                     /locus_tag="OUB02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8884686.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(7990..8601)
                     /locus_tag="OUB02_000911"
     CDS             complement(7990..8601)
                     /locus_tag="OUB02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCT8884687.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8591..10954)
                     /gene="lon"
                     /locus_tag="OUB02_000912"
     CDS             complement(8591..10954)
                     /gene="lon"
                     /locus_tag="OUB02_000912"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCT8884688.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11134..11646
                     /locus_tag="OUB02_000913"
     CDS             11134..11646
                     /locus_tag="OUB02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HCT8884689.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11634..12161
                     /locus_tag="OUB02_000914"
     CDS             11634..12161
                     /locus_tag="OUB02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HCT8884690.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12232..13482)
                     /gene="clpX"
                     /locus_tag="OUB02_000915"
     CDS             complement(12232..13482)
                     /gene="clpX"
                     /locus_tag="OUB02_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCT8884691.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13501..14085)
                     /gene="clpP"
                     /locus_tag="OUB02_000916"
     CDS             complement(13501..14085)
                     /gene="clpP"
                     /locus_tag="OUB02_000916"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCT8884692.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEIFSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14235..15521)
                     /locus_tag="OUB02_000917"
     CDS             complement(14235..15521)
                     /locus_tag="OUB02_000917"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCT8884693.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRANLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15715..16185)
                     /locus_tag="OUB02_000918"
     CDS             complement(15715..16185)
                     /locus_tag="OUB02_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8884694.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMNIEEDDIKIEEIRI"
     gene            complement(16185..17534)
                     /gene="rph"
                     /locus_tag="OUB02_000919"
     CDS             complement(16185..17534)
                     /gene="rph"
                     /locus_tag="OUB02_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCT8884695.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17694..19718)
                     /gene="ligA"
                     /locus_tag="OUB02_000920"
     CDS             complement(17694..19718)
                     /gene="ligA"
                     /locus_tag="OUB02_000920"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCT8884696.1"
                     /translation="MSIKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKASDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(19904..20842)
                     /locus_tag="OUB02_000921"
     CDS             complement(19904..20842)
                     /locus_tag="OUB02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCT8884697.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(20944..21366)
                     /locus_tag="OUB02_000922"
     CDS             complement(20944..21366)
                     /locus_tag="OUB02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HCT8884698.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21554..22837
                     /locus_tag="OUB02_000923"
     CDS             21554..22837
                     /locus_tag="OUB02_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HCT8884699.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     KISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(22915..23430)
                     /locus_tag="OUB02_000924"
     CDS             complement(22915..23430)
                     /locus_tag="OUB02_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCT8884700.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23430..24806)
                     /locus_tag="OUB02_000925"
     CDS             complement(23430..24806)
                     /locus_tag="OUB02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HCT8884701.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(24831..25382)
                     /locus_tag="OUB02_000926"
     CDS             complement(24831..25382)
                     /locus_tag="OUB02_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCT8884702.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25419..25592)
                     /locus_tag="OUB02_000927"
     CDS             complement(25419..25592)
                     /locus_tag="OUB02_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884703.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDENN"
     gene            complement(25603..26436)
                     /gene="fdhD"
                     /locus_tag="OUB02_000928"
     CDS             complement(25603..26436)
                     /gene="fdhD"
                     /locus_tag="OUB02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCT8884704.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26485..28629)
                     /gene="fdhF"
                     /locus_tag="OUB02_000929"
     CDS             complement(26485..28629)
                     /gene="fdhF"
                     /locus_tag="OUB02_000929"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28213..28215),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCT8884705.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(28880..29938)
                     /locus_tag="OUB02_000930"
     CDS             complement(28880..29938)
                     /locus_tag="OUB02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCT8884706.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         9009 a         5387 c         3280 g        12292 t
ORIGIN      
        1 tttaagttta taagcttttg taatatatca aaatttatat ttatagtctt tggattcata
       61 tttagatttt ctgaaatgtt cttaattaat tgaataatac tctttgggaa aagaattact
      121 tgtatattca atttatcatt tttagaattt ataactttat attttattga atagttattt
      181 atgtttattg gaatgtattg tgttatttca aattcaatca tgcttaatat gtctcttttt
      241 ttcttagtat ttagaacttc aacatttcta atgactatat cctgaatttg aatgttaaag
      301 tataaatttt tatataatat ttttgattta ccaatctcct cttttacaaa tctaactaca
      361 tcttctatat atttaatctt ctcaatcgaa ttactatcat tttttatatc aaagttaatc
      421 tcttttataa ttgatttatc tataaaaact tttttatgac tttttcttaa aaatacaagc
      481 cttatataat catcatttat ttctaagttt agaatttttt taattatagc catctccttc
      541 ttaaataaaa ataattttaa aacttacagc aatcaatata aaatattatt ttggataaaa
      601 ttgcttagaa tttatattta cgcttatatc tgttccgcta tcatttatga caagttcgaa
      661 tttacctgat gtactctgag gaactggaac agtatttagg tatccctttt ccttcaatgt
      721 ctctacactt agagagtcta ttgtcttttc atcatcatta attgccatgc tagctgcggt
      781 tactatatta gcagcattcg tatagtctgc atttaatttg gcactttctt gaaccttact
      841 atacttaact aatgctatac tagatataac gcctaaaata gttactacta caatcatttc
      901 aacaagtgta aatccttttc gttttttatt tatcaaattt atcaattaaa tattctctcc
      961 ttttcaaaag tttcatctaa taaaatttat ttttctctct aaaaattagt aattgcatca
     1021 aacataggaa caaccattgc taaaataaaa gtaccaataa ttaatcccat aaatacaatt
     1081 agcataggtt ctataaattt cattatttgc tctattttgt tatttaattc attttcataa
     1141 aatttattta ttacatttag acttttatct agtttcccac tttcttctcc tgtatttgcc
     1201 attgaaataa ataattttgg aaaaatattt gctaaattca aagatttacc tatgctattt
     1261 ccttttttta tataattatt ggaaatcttt aacctttcat aaataaattc gttgtctaca
     1321 acactagctg acatgtctat agcttccaca atttgtattc cactgctaat taaaacggac
     1381 aatgcccttg aaaatcttgt tgtagctact aactgattaa ttttttttat aaaaggtatt
     1441 ttgaatttta acgaattaat caattttttt atattatcat ttgttataaa gaaataaatg
     1501 tttcctattg aaaacaatat aaaactaaat attatataag ctaagttatt tgtaaccact
     1561 atagatattc ccattaaaac tctagttatt aaaggtggtt caattccatt gttggtaaaa
     1621 accacctgaa aattaggtat tacaaatacg aatataaaca acattgataa gatagataat
     1681 attataagta ttattggata tattgaaata gttattattt tcatttttaa tttgtattct
     1741 ttatcataat aattagataa atcactcatt attatatcta agtttccact agtttctcct
     1801 gcttttatca tacttataaa gaatttagaa aatatacctg ttatttgaaa tgattctgct
     1861 attgagttac ctttttgaat atgatttgat actatactaa gcaaattctt tactttttta
     1921 cttgactgac tctcaatgat ttcaaatatt tttgtaattt cacatccaga ttccagcaat
     1981 atgcctatct ctttgcaaat tactctaatt tctttatcac tgattctatt taatactaaa
     2041 taaaactttt ctaaattata aaatatattt ttagataatc tcatgtacta taataccttc
     2101 caaattatat taattacaat taaataaact taatattttc taatttaatg catttaattt
     2161 tatttcactt aaatataatt tatactcaaa catcttaatt aaaatagatt aaataaagac
     2221 aactgaccaa acgaagttta actatacaat tcagttaaat catttcattt actactaaac
     2281 actcttctaa tgtcgttatt ttttcattta ttagtctttt acaactatct tcaaatgtta
     2341 tcattccatt attttgtgcc acatctttaa tagtactaga atcctccata tttcttatac
     2401 atgtctttat atcatcatta atttctaaga tttcataaat tgctgttctt ccaaagtatc
     2461 ctttatcatt acatttttca caccctacag ctaccttagt atttacatct ccactaaaat
     2521 tatcttttat cataatctca tggctacaat gattgcagac ttttctaacc aacttttgag
     2581 atataacacc tataagagag gcatttaaaa gatatggtgg tatttgcatt tctagtaatc
     2641 tagctatgga agatactgca tcattagtat gtaaagtact tataactaaa tgacctgtta
     2701 tagctgctct tactgctatc ttagctgttt ctgcatctct tatttctccg accattatta
     2761 tatctgggtc ttgtcttaat atcgctctaa gacctacatc aaagctcaac cctacttttg
     2821 aatttacttg aatctgactt atgccatcca tcttatactc tacaggattt tctatagtca
     2881 taatattttt atttattcct tttaaatcat ttaataagga atatactgtt gtagtctttc
     2941 cactacctgt aggacctgtc actaataata ttcctgctct tttatttatt atacgattta
     3001 tagattttat agcttcctct gaaaaaccaa gttctaattt gtcttttaaa aaagtatctc
     3061 tattaagtat cctcaaaact attttttcac catagacagt tggtattgat gaaactctaa
     3121 tgtcaattaa atttccacct aactttatat ccaccctacc atcctgagga agcctttttt
     3181 ctgttatatc cattgatgcc tttaatttta taaccgtaga caatgatgga tagacatcaa
     3241 ctaaaaattt tgatacttcc tttagttctc catctaccct cattctcact attaaatgtt
     3301 ctttaaatgg ttctatatgt atatcacttg cgttttcttt tatagctttt tctaaaagat
     3361 tttcaaataa tttttttgca taatcctcat caagttcaaa gctattataa tatttttcaa
     3421 tcttttcact tataagttct tttttctcct tcttcaatat aatttcttta cctgtagcta
     3481 atctcaagtc ctgtagagca taaatatcct catcttctat ttctacaact aaattatcat
     3541 cttgaaactc aactggaaat acattatatt tttttgcaag tttttctggt attaacattg
     3601 gcaaactgct ttcctctaat ataatcttaa tcacccttta tatactttaa tttattaggt
     3661 atattcattt ttttcataga gatattttct tcagttttta tgcttatttt cattggataa
     3721 ttggtttgta tgctatctaa atatttacta cctttatcta cataattaga tagtttctcc
     3781 ttatctccaa tgcactttat ttcatatggt tgtgctacag gaactccatt tatatttata
     3841 tggcttcctg caagtacaac ttctgaatat tgattaattc tttgaccatt tatttctatg
     3901 tattttcctc cgtttacttt taattcattt ataactttta caagaatctt attatagtct
     3961 atactattag ctatattccc tgttttttca tctatagcat ctatacttaa tattatccca
     4021 ctacccttta catctgaata tgatagacta tctctaagtg tttctatttc tgatatttct
     4081 tcagaatttt tttgattttg tttcattgaa ttaatttctt tatctaactt gtctttctct
     4141 ttggttaatt cttttataga ttttcttgtc atttttattt cttttcttat tgacttgtct
     4201 ttgcttgtat aaactgtttc ttcattgtta taatctctaa atatggttcc aacaagtata
     4261 cctaaaatag tacttgctag tattataaac acatattgtt ttttcatact gtattgctcc
     4321 ttacaaattt atttttatat gataattttc tttaattaaa tattaaacat ttacatatgc
     4381 ttattatatc aaagttatat attaaaattc atctttagta tataatatca tacattgaac
     4441 tcctatcact aatttaccaa tatcaagtat gcatttttag atgttcttaa aattatataa
     4501 actttcaata aacattgttg tgatattata aaaaaacata aattaggaac ggaaataata
     4561 attatgaaaa acaaataatg actttggaga ttattaatat cattttttag caaaaaaagt
     4621 aaaatggcat agcctcaaag ctacaccaat ttactaaaaa ttatattatt attaacatta
     4681 attttaaaca tatacgttta taagttttcc tcatatttta aaattctttt aattaactta
     4741 tctcttaatt tccctacttc atcaaatctc atattaggac tatagtaact ttctataata
     4801 tcatgtttag acttggctct cttaagattt agtactgccc aacaaattaa atcatctaaa
     4861 actttttcat caaatttaat atcttcttca tatttaagta gtttttctct atttaaaaac
     4921 tgatttaaat caattgtatc ttcttcttta aaagtcgtac ttatagttat gcctatatct
     4981 aaatcagtta atagtacagt ttcaattttc tctggaataa gtggataatg ataaacttct
     5041 acatttaaac ctttttcaat agctttctta tatactttgt ttaataatgt agttttccca
     5101 gttcctattt ctccatctaa ataatagact ttctttacat cttttaaaat gttttctgta
     5161 taatctaaac accctacagg agttatagct gaaccaaata aatgcttttc tctttttaga
     5221 tttcctgtgc ttttaactcc atcaaataac ctttttataa attcttctgt gaccaaattt
     5281 acttttccat aatccataca atcagaatat ttttcttcta tgtctctaaa gattggttct
     5341 gctgatttta aaaacttaaa tgctctttta aatgaattac taatatcttt atcataaact
     5401 gatatttctt gtttattctt ttctaattct tctgtattcc agtaatcacc taaattaatt
     5461 atctcatcca aagctccagg tagctttggg tccatagtat gtggggcagt tgcatccata
     5521 agtaccacgc ctaatttctt tataactaaa ccatccaacg aacttgggtc agaaggacaa
     5581 tgatataaat caatatcata tcctctatgc aaaaactctt tataaatttt tttcataagt
     5641 gaggattttc ctactccagg tccacccttt aaacaaaaaa ctctttttac attttcagga
     5701 attatatagt caaaaaaaga atatgagcct tgtgatgtat ttccacccgg gaatgccttc
     5761 tttacctttc cttccatatt agcgcctcct cataatttac aatatctctt aattaagata
     5821 ttcatattat aagaatttta ttacagcttt ctgattttga taaatttaaa acttgccatc
     5881 taatactaat aacaggttaa ttttatctaa ttattataga ttctcatact gttccttatt
     5941 ctatctataa atacaattta agtgtccata ttgaaatatt tgtattgtaa tacagctgga
     6001 tattacttaa atccaattgt ttccattata attttatgtt aaaataatat tacaaaatac
     6061 atctgttttt cttcataaac gggtgaaatt ccctatcggc agtaaaagcc cgcgagcctt
     6121 atggcataat ttggtcatat tccaaagccg acagtaaaat ctggatggta gaagaaaata
     6181 gtatatgagt acctttatgt aattttacat gagtaatcta tacaaatcct tcaactaccg
     6241 tatttattca tgaaattaga catattcaag gtacctaata tacaggtgct ttttttgttg
     6301 tttattttac aattatatcg tacttataaa atctattaag attggagtgt tatcatgaaa
     6361 caaaaatgga tagtattgat tatcatctgt attggtgtat ttatgtctac tcttgatgga
     6421 agtatactaa atatcgcaaa tcctacaatt gctgctgact ttaaaattaa catgtcacaa
     6481 attcagtggg ttgtaacagc ttatatgctt gttgtaacag ctactatgtt atttttcgga
     6541 aaacttggcg ataaagtagg ttcaaatcgt ctatacacat taggattttt tatatttaca
     6601 ataggttctt ttctgtgtag tatgtcaaac aatttatcaa cattaatttc ttcaagaatc
     6661 tttcaggcag ttggagcaag tattttaatg gctactgggc ttggtatagt ttccaatgca
     6721 tttccagcaa atgaaaaagg aaaagctata ggaatcactg gagctgtagt tggtatagga
     6781 aatatgagtg gtcctgtaat tggaggtata attttagaac attttggatg gccttctatt
     6841 tttattatca atatacctat tggtattata gcagtctttc ttggcattaa atttctgcct
     6901 aaacctgttt tggatgaaca aaacaagagc tttgatattc caggtcttct acttttcgca
     6961 tcttgcacta cgcttatatt acttgcaatg aatgagaagg gtaatacaag attgtattta
     7021 ggtataacag cacttataat cttcttattg ttggcattaa gagaagtaaa atttgaacaa
     7081 agctttattg acctacctct atttaaaaat agaaacttta ctgtaggcaa tatcattgga
     7141 gtagcttgtt attttcctca aatggcagta tcttttctac ttccatttta tttagaacaa
     7201 ttaaaaaatt tatctcctat gatggctggt tatgtaatga ctgtacatcc acttattatg
     7261 gtacttatag cacctattgc aggttcactt tctgataaac acggtgcaaa aaacattcta
     7321 acagcatctt tttcatttat gaccatctca ctagttggaa tggcactttt aaaagcagat
     7381 tctccattgt atcttttaat tgtatgttta gttatttttg gacttgggct aggagcattt
     7441 agttcaccaa acaatagtag tattttagca gatgttcctc ctcaaaaaca gggatacgga
     7501 ggcagttttc ttgcaactat tcgtaattta tcatttgctc ttggaacagc attttttagt
     7561 agtttttttg cacaaagtct tacatacaat caaaagttta aaagtcacac atcagcctat
     7621 gttatagcta gcaatcagtc ttattggatt gcagcatcag tatgttttat agggcttata
     7681 ctaactgttt tctttatgag aaaaacagat aaatctattt cttaaatgct atttattgct
     7741 gactacataa gtcaataatt ttttagttga actaatttat actaattttc ccatacctta
     7801 ctaaaattta ttagtattta gcaaaattac tgatatttat tgtttataaa caataaggat
     7861 gccaaaaaaa gttcattaaa actattttaa ggcatcctat attttgcttt aatattaata
     7921 taattaaaac taactattat tacatttatt attcaaacaa atcttatgtc aatatttaac
     7981 attttaaatt taatctactg ttatttcata tccatgttga ttgtattgat ttacaatctc
     8041 ttcccataat tcttcaacac cagtcttttt tagtgaagaa acaggaatta ctttttcact
     8101 actttccatt tgcaattttt ttcttattat acttatgtgt ttttgatatt gtcctctaga
     8161 tattttatct gccttagttg caataactac acagttatat ccaaagtgct ttatccattc
     8221 atacataagc ttatcatctg ctgtaggctc atgtctaata tctacaagta ggaatataga
     8281 acataactct tgtctacttt ctagatatcg ttccattata cctccccact tttctttttc
     8341 agacttagct atctttgcat atccatagcc tggaaggtct acaaaataaa attcattatt
     8401 tattagataa aaatttatag ttctagtttt tcctggtgtt tgactggttc tagcaaagtt
     8461 tcttctgtta agcaatgtat ttattattga tgattttcca acatttgacc taccagccaa
     8521 tgctatctct gggattcctt ctgctggata ctgtgattta ttcacagcac tcatagttat
     8581 ctctgaactt ctaattttca ttattttcac cactctttaa taaagcttgt tctaatactt
     8641 catccatatg ttctacaagc acaaactcca ttttttcttt tacattttct ggtatttcat
     8701 ctaaatcagc ttcacattct ttaggaatta atacttttgt tataccagct ctatgagctg
     8761 ctaataactt ttctttaact cctcctactg ctaagactct acctcttaaa gttatctctc
     8821 ctgtcattgc tatatttcca ggcacaggtc ttttagtaag tgctgagatt actgcaagag
     8881 ccatagttat accagctgat ggtccatctt ttggaacagc accttctgga atgtgtatat
     8941 gaatatcatt cgtcttataa aattcagggt ctatatcaaa cttgtccact attgacctta
     9001 tataagagat acctgtcttc gcagattcct tcataacatc tcctaatttt ccagttaata
     9061 caatttctcc tttaccttta agtacgttta cttctacttc aagtgtaact ccaccaactt
     9121 ctgtccatgc aagaccattt actaatccta tttgagggtt aacttctgca agttgatact
     9181 taaacatgtc ttttccaaga tatgtctcta ggtcagactt atttataaca acctcttcaa
     9241 gagttgggtc ttcaacgtat ttttttgcaa cctttctaca aatcttacca atagttcttt
     9301 ctagtgttct tactccagct tctcttgtat aatggttgat tatacttctt aaagtttcat
     9361 catctatttt tataaagctt tcttttaaag catgttcttt aatctgtttt ggaagtaggt
     9421 atttttttgc tatatttaat ttttcttctt ctatataccc agatacttct attacttcca
     9481 ttctatctaa taatggtctt ggtatattac ctagactatt tgctgttgta acaaatagta
     9541 tttttgataa atcaaaaggt atttctaagt aatggtccac aaaatcctta ttttgctctg
     9601 ggtctaatac ttctagcatt gctgaagctg ggtcaccttt ataatctgct gccatcttat
     9661 ctatctcatc aaataagaat acaggatttt ttgtttgggc ttcttttacc ccattaatta
     9721 ttcttcctgg aattgaacct acataagttc ttctatgacc tcttatttct gcttcatctc
     9781 taactccacc caaagatatt cttacaaatt ttctatttaa tgctctggcg attgatttta
     9841 ctatagatgt ctttcctgtt cctggaggac caactagaca tattatagga cctttaagtg
     9901 acttagcaag agttcttata gctagatact ctagtattct ctccttaact ttttctaatc
     9961 cataatggtc ttcatctaat atatcttttg ccttagttat atctaattta tcttttgtct
    10021 ctttattcca tggcaatgag aatatagtat ctaaatagtt tctacttaca gatacatctg
    10081 gagacataga agatattttt gaaaatttat ctatctcttt ttctattttc tctttagttg
    10141 tcttaggtgc ttttattttt ttaagctttt ctctatactc atctgcttca gagtttatat
    10201 cttcttcttc acctaattct ttctgtatag cttttaattg ttctctaaga taatattctt
    10261 tttgaacctt attcatctgt ttttttactc ttaaagttat tttcttttct atttttaata
    10321 tatctatttc ctctaataat atagaataaa taagttcaag tctttttcta atatcaaatt
    10381 cttctaatat ttcttgtttt tggcttgatt ttagatatat gtttgcagct atcgtatcta
    10441 taaatctatc tacatcttct atatcagcta aagatattaa tatttctggt gataccctat
    10501 ttccaatatt tatatactct tcaaaggcat caaatacatt tcttacaaat gcttcaattt
    10561 caacttctgt ttcactatcc aaattatcac tatcaaatac tatttcttct attactgctc
    10621 tgaaataccc atcttcttgt tctattttct ttactctacc ccttgataca ccttcaacca
    10681 aaacacgaac tgtatctcct ggtaatttta tcatttgctt aactttacag attgttccca
    10741 catgataaaa atcctcttct cctggctcat ctacctctgc ttctttctga gaagttaaaa
    10801 atataagttc ttcatccatc atagcttggt ctaaagcttt aagagatatc tctctaccta
    10861 tatcaaaatt taatatcata tatggaaata tagctaatcc tcttagagga attaatggta
    10921 attcatgatc tattttagta taattttgtt ccatcaacat tactcctttt ctagtctaat
    10981 tgctttgctt ttaatattat atatttgaac aagttaattt tcaacattaa tgataaattc
    11041 ttagaatact atcatactat attatctttt tattatgata taatattaat attagtatac
    11101 ttagatgtta tttatctttt ggaggtttct attatgttga aattgttttt aaccttttta
    11161 aaaattggtg cgtttacatt tggtggtgga tacgctatgg ttcctcttat acaagatgaa
    11221 cttgtaaaca aacaaaaact tttagagcag gaagaattta ttgattattt atccatagct
    11281 caaagttatc ctggagtact ggcagttaat atatctgtat tattaggata taaactttat
    11341 ggtataccag gagttcttat atgtacctta ggctctgctt taccatcatt ttgtatagtc
    11401 ttagtcttat cgtatctata ctttaaaaat tcatcatcta aaattttaga tggatttttt
    11461 aaaggagttg cgcctgttgt aatagctctt atattgtatt cttttacgag catgttcaag
    11521 aaactaccaa agtctaaaac taacatacta ctattaatta tagcagtttt atctgttggt
    11581 gtattcaata taagccctat ttatttaata attggaggag gtgtatatag tctatgtcag
    11641 aaataatact actcttctta atattcctta aaataggggc atttagtttt ggtggcggtt
    11701 atgccatgct accatttatc cagcaagaat ttatatctaa atatcacttt ataactaatc
    11761 aagagttttt ggatttactt gctatatcac aatctacacc aggtcctgta gcaataaata
    11821 gtgctacatt tgtaggattt aagactgctg gcttctttgg ttctctagct gcaactattg
    11881 gtgttacaat ttttgcatta ataggtctaa ctattatatc aaagttattt tctaaattta
    11941 aaaataataa aaaggtagaa aatttattga cagttcttag accaattact ctaggattca
    12001 tattatcagc agctttttca tctgccaaag aagttaattg ggatttatat ggaataattg
    12061 catttatagc atcattttat cttttatata aaggaaaaat tggttccatt gcaattgtat
    12121 tcgtctttgg tattctaggt atattactca catttttata ataatgatac ctattttata
    12181 tttagcatat taacataaaa aaaggagctt ttaaagctcc ttttcatcat cttatgcact
    12241 ttcctcttga tccttaagta ctatgatagg atttactgag tcttcgttca cagatttctc
    12301 tgttactata accttcttta tattatctct agatggtact tcaaacatag tttccatcat
    12361 tacgctttca actatacttc taagtcctct agctcctgta tttctttcta tggccttttt
    12421 agcgattgca cgaagagcac cttcttcaaa ttctagctca acatcatcta actccaataa
    12481 ctttttatac tgttttacta gagcattttt aggttcctgt aatatctgca ttaatgcatc
    12541 ttcatccaac aactctaaag tagctaaaac tggtattctt cctataaact ctggtattat
    12601 accatatttt aataaatcct ctggaagtac tttttcatat agcttcccta aatctaattc
    12661 ttttttactt tctattttag ctccaaaacc tagtgtttta tctccaccac gtttttgaat
    12721 tatcttctct aatccatcaa aagctccacc taatataaat aatacattag tagtatctat
    12781 ctttaaaaat tcttgatgag gatgttttct tcctccttgt ggtggaacat ttgcaacagt
    12841 accttctaat atttttagaa gtgcctgttg aacaccttca ccactaacat ctctagttat
    12901 agatgggttt tcagattttc ttgcaatctt atctatctca tctatatata ttatacctct
    12961 ttcagctttt tctatatcaa aatcagcagc ttgaataagt tttaaaagta tattttcaac
    13021 gtcttctcca acatatccag cttcagtcaa tgatgtagca tctgccattg caaaaggtac
    13081 atttagagtt cttgctagag tttgagcaag taatgtcttt ccagaacctg taggtcctaa
    13141 taaaagtata ttactctttt gaatttctat atccttacta ctagattttt tactgtatat
    13201 tctcttataa tgattataaa ccgcaactga taaagctttt ttagcttttt cttgacctat
    13261 tacataatca tttagaatct ccatcatttc ctttggtttt ggtaaagatg tagtatcttc
    13321 atctatagta tcttctattt cttcctgaat aatttcgtca caaagttcaa cacactcgtc
    13381 acaaatatat acatttggac ctgcaattaa tcttcttact tgatcttgat ttttaccgca
    13441 gaatgaacac tttaattgtc gtttttcttc atatttagac atattaacac ctctctctat
    13501 ctacggtctc tttgtaaata cttcatctat taaaccatat tctttggctt ctagtgcact
    13561 catgaagtta tcacgttcag tatccatttt aattttttct aatggctgac cagttctttc
    13621 agagaatatc tcatttagag tctcttttat ttttaaaatt ctctttgcat gaatttctat
    13681 atctgttgct tgaccttgag ctccacctaa tggttgatgt atcattattt cactatttgg
    13741 tagtgctagt ctttttcctt ttgctcctgc tgccaacaag aatgctccca tagaagcagc
    13801 catacctata catatagtgc ttacatctgg tttgatgtat tgcatagtat catatatagc
    13861 cattccagaa gttatacttc ctcctggaga atttatatat aaatgtatat ctttgtctgg
    13921 gtcttcagct tctaaaaata atagctgtgc tactataagc cctgctgtgg catcgttaac
    13981 ttgatctcct aagaagatta ttctgtcttt taatagtcta gaaaaaatat cataagatct
    14041 ttctcctctt cctgtttgtt caacaactac aggtactaat gccatggttt tataccccct
    14101 ttattctata taatttacag tagctttttt taagctactg taaattatta tacgcattta
    14161 actaaattat atttgcacat cattatatac taaacatttc ataacgtcaa ctatattagt
    14221 tagcgtttat tttattaagc tattgttgca ttatcaacta ataaatctat tgtttttctt
    14281 atttttattt gaccttttat atcttctttg tcagcatctc ttaaagaagc ttctattttt
    14341 tctacttcca tattgtaagc agaagccatt ttttctaact cagccttaaa ttcttcttct
    14401 gttgcttcaa ttccttcagc tttagttatt gcttctaata ctaaagaaga cttaactaat
    14461 tttttagcat cttcttttct ctcttctctt aattcttcca tagttctacc agtcatctct
    14521 agtaattgtt gtaatccaaa tccttgatat tgtaattggt agttaagttc atttaacata
    14581 ttatctattt gatgttgtat cataacttct ggtatttcta tttctgtatt ttcagcaact
    14641 ttttctacta ctgaatttct agtttctgca tctgctctat ttttagcttc ttcttctaaa
    14701 ttagctctta catctgcttt taattcatct aaagaatcaa attcactagt atctttagca
    14761 aattcatcat caagtgcaga taattctttt acttttacat catttatttt tacattaaat
    14821 acaactggtt ttccagctaa atcttgtgag tgatattctt ctgggaaagt aacattaact
    14881 tctacttctt caccagcttt tttacctact aattgttctt caaatcctgg tataaatgtg
    14941 tttgaaccta taactaagtt ataattttca cctttaccac catcaaaagc tacaccattt
    15001 tcaaaacctt cgaagtctat tatagctgta tctccatctt ctaaagcttt gtcttctaca
    15061 cttactaatc tggcattttt ttcaaccatt tcttgtagtt tagcatcaac attttcgtca
    15121 cttacagtat tctcaacctt agctatttct atacccttgt agtttcctaa ttcaaactct
    15181 ggtttaactt ctacattaag aaccataacc aacccattgt ctttacttat ttcttctata
    15241 tctaaatctg gattgtctat tgggtctatg tctaattcct tgatagcttc tggataaact
    15301 tctgggaata atatttctat agcatcattg tagaatatac cttttccgta ttgagtctct
    15361 ataacaattc ttggagcttt accttttcta aatccaggta tattgtattt atttctgctt
    15421 ttattataag ccttatttac tgctgcttca aatttattgt tatctactgt tatttttaaa
    15481 gtaactttat taccctcttt tttaattagt tctgctttca tccttctaat cctcctagtg
    15541 atatttatgt aatacccgat aacggtatta taattcattc tatttataat tcaaccactt
    15601 aattatatca cataagtttt tttatttaca agtgttccat taatatcgtt gtacttattt
    15661 gataaaattt aattttttga gcgttttttg ccattttttt agataaatac acctttatat
    15721 gcgtatttct tcaattttta tgtcatcttc ttctatattc ataatgacaa aacttttata
    15781 cgatactcct cttggaagtg aagtgcttcc aggatttatg taaagtacac catctttaat
    15841 ttctctaaat ggggtatggg tatggccaaa tataactata tcagcatcaa cttcagttgc
    15901 ctttttttct aacatattag ttccatattt tacaccatac ttatctccat ggcataagaa
    15961 tatggtttta tttgccactt caaagacaac ttcttcctca acagcatcaa agtcacaatt
    16021 acctttaact gcaataatac ctacattagt catgctatga atatatcttg aatcagtaaa
    16081 gttatctcct gcatgaatta ttaggtcaca ttctttcaaa tatggtattg ttttttccat
    16141 atttttaatc atcatatgtg tgtcgcttac tatgcctatt ttcattactt cactaccctt
    16201 tttataaact cttgtttcat aagttttagt gcgtttgctc tatgactgat tgaatttttt
    16261 ataacactag gtatttcacc aaaagtctta tcatatcctt ttactataaa taagctatca
    16321 tatccaaaac catttttacc tttttcttca aatcctatca ttccttcaca aattcctctt
    16381 actacaaatt cttttccatc aggaaaaact actgctattg cagagacgaa tcttgcattt
    16441 cttttgctca ttggtacatt ttgcatagct tttaacagtt tttttctatt ttcttcatct
    16501 gttgcatgtt ctcctgcata tctagctgaa taaacaccag gttttttgcc taaagcatct
    16561 acttctaagc cagaatcatc tgatattgct ataagtttag tcttttttgc tattgttcta
    16621 gcttttatca acgcattatg ctcaaatgtt tttccatctt ctactatctc tatacctgct
    16681 aaatcaacat cttttaaaga gtacacttta tattcaaaat cttttaagat ttcacctatt
    16741 tcttctagct tatgagcatt attagtagca atcactactt cattaccata atccataccc
    16801 aatatctcat ctgctatttc acctaaagct tctctttgaa gccttattaa ttctttattc
    16861 cctttttcac ctaactctaa caataaattt aaatctgctc tcgaaaaagg tttctcttct
    16921 cctgtcccct gtacttctac aaactcacac ttatcagtca ttattatatt catatctacc
    16981 tgtgctttag aatcttcttc ataacataaa tctagcatgt gtattccatt tacaatacca
    17041 acactaattg cagatacaaa atttgttatt ggcatctctt ttattgtctt tgctttatat
    17101 aatttatata ttgcttctgc aactgctaca aatgcccctg ttatagatgc agttctagtt
    17161 ccaccatctg cctgaatcac atcacaatct acccaaatag ttctttctcc taatttattc
    17221 aagtctacaa cagaccttat tgctcttcct attaacctct gtatttcttg tgttcttcca
    17281 tcaatttttc ccctagatgc atctcttatc tttctttgtt gtgttgctct tggtagcata
    17341 gaatattcag catttatcca tccagtacca ctatttctta aaaaaggagg aactttttct
    17401 tccactgatg cagtgcatat aacctttgtc tctcccattt ctataagaac agaaccttct
    17461 gcatactttg tataatctct agttatcttt acaggtctta tctgttcatt acttctatca
    17521 taacttctgg tcatttttta tatcctttct catatttttt attttttata aatttatttt
    17581 tataattttt aattttgtca aatattaatt attgtcattt ttcgtaatct tattagctta
    17641 ctgaactatt ctatcattaa ttatagtagt tttctactaa attaagcgac tttttaactc
    17701 tctaatacag cttttacttc atctttcgat gataacttta ataattctaa aaatctctct
    17761 tcatctataa ctttaattcc taaatcactt gctttagtaa gtttagaacc agcttcttca
    17821 cctgccaaaa caaatgttgt cgatttacta acactagatg tagcttttcc acctcttttt
    17881 tctatcatat cttttgcatc atttcttttt aatgtaggaa gtgtaccagt caatacgatt
    17941 ttcatccctt caaatatttt ttctactttt tcacctgtat tatccaatga ttttgtattt
    18001 actcctacat tttttaactt gtttataacg ttcatatttt tgtcttcctt aaaaaactcg
    18061 acaacgctgt ttgccattat acttccaaat tcctctaatt cttctagttg ctcactagtt
    18121 gcagaaatta cttcatctaa atccttaaag ttatatgcaa gtatcttagc tgctttaact
    18181 cctataaatt taatacctaa tccatttata agtctccata aatcattttc ttttgatttt
    18241 tcaattgaat ttattaaatt gtcagcagat ttttcgccca ttctctctag gtcgactaca
    18301 tcttcctttt ttatataata taaatcagat acatcttgaa taattttttc atttaatagc
    18361 agtgtaatta tagattctcc caatccatct atattcattg catcccttga cacaaagtgt
    18421 ataattcccc ttcttatttg ggctgggcaa gacatattga tacattttac agcagcctct
    18481 ccttctaatc ttattgttgg ctctgaacaa actggacact tttctggcat tttaaaatct
    18541 atttcatctc cagtacgttc ttcttttata acttctaaaa cttgaggtat tatgtcccct
    18601 gctttttgaa ctaatactgt atcatttatt tttatatctt tttctcttat ataatcttca
    18661 ttgtgaagtg tagctctact tacagtagta cctgcaagtc tgactggttc aagaattgct
    18721 gaaggtgtta ttgtaccagt tctcccaact tctactatta tatctactat tttagttttc
    18781 ttcttttctg ctgggaattt atatgcaatt gcccatctag gacttttggc tgtatatccc
    18841 atttcgtctc tttgttttat attgtttact tttattacca taccatctat ttcgtagtct
    18901 aaattacccc tgttatctgt ccagtattca atatgctcaa taacttcatc tatactttta
    18961 caaactttat aactctgact tactgaaaat cctagttttt ctaagtactc tagtgattca
    19021 ctgtgacttt ctaaatcgta ttcttctata ttttccatat taaatacaaa tatatctaat
    19081 ggtctctttg ctgttagttt tgggtctaat tgtctcaatg aaccagctgc aagattcctt
    19141 ggattagcaa ataatggctg ttcttgttct tcttgttgtt catttacttt tctaaaatta
    19201 tcttttgata tatatacttc tcctctaact attagttctt ttttatcatt tatcttaaga
    19261 ggtatgcttt ttactgtcat taagttttga gaaatatctt ctcctacaac tccatctcct
    19321 ctagttgctc ctttttcaaa ctctccattg ttataagtta gacctacaga aagaccatct
    19381 attttaaatt caacaacata ttcaacattt tctcccacat attcttgaac tctcttatca
    19441 aaatctctca aatcttgctc tgaaaaagca tttgataaac tcaacatagg agttttatgt
    19501 acaatttgat tgaacttatc taatggctta cctcctaccc tatttgttgg agaatcaatt
    19561 ctctttagtt ctggatgttc ttcttctaaa gaaattaact ccttcattaa cttatcgtat
    19621 tcataatctg atatttctgg gctatcttga ttataatacc tttcattatg gtagtttatt
    19681 gtctctataa gtttattaat ttcatctttt atgctcactt tttgcacctc ttcatatttt
    19741 taaaattaaa ttattttgaa atttccctat aattaattat ctcctattta tatatttttt
    19801 agtatatcac ttttttggct ttaaatttat tacaaaaaaa actcttatta gtatctttaa
    19861 tacgactaat aaaagttttt atttttatca aaatttttta tttttaatag cctcttgata
    19921 tgtctacaac attatctaca ggtttattct ccattatata gtgtttcaaa ttattataaa
    19981 ccatattaaa tgttctctct ttgtttttat ctgacaccca agaattatga ggagttacta
    20041 tgacattttc gcactcccac aacttattgt ccttgtttaa aggttcactt tcaaatacgt
    20101 ctaatgctac ccctctaaat ttctctacac attcttctaa atctttctga ttaactgtat
    20161 ttcctctacc tacatttata aatacagaac catctttcat aagtttaaac atatctttgt
    20221 ttactattcc atctgtactt tttgtagaag gcattgctac aactactata tcacattctt
    20281 caaatacaat attcatatcc tcagtagcaa aacacttatc aaagtatttt ttactacttc
    20341 catcagtatt gacaccccat atttctacac caaaagcttt taatcttttt gctgcttctg
    20401 aagatatagt acctgttcct aaaaatccaa ctctttgtcc atacaattct gatacagaaa
    20461 aatccatctt ccattcttta ttttgttgtt gtttaaaaaa ttttgcacta tttttatata
    20521 tttctaaaat atacaataca atactttcac caataggtac actatatccg cctctattat
    20581 tagctatctt aatatttctc ttcaatacct cttcttttgg aagttggtct atacctacac
    20641 tagttgtctg tatatattgt aaatttttca tcttagaaat atctaatctc tcaaatggat
    20701 tgtatgtaac tagtacatct gctgtattta catcctcatt attttcaata ctatcttcag
    20761 agtaatgtat gacatcatag cctaattctc ttattttatc aaatttttct tttccataat
    20821 ttattgtaaa taaaactttc atctcaaatc tcctccatct acccacattt aaaattttat
    20881 attctaaaat ataatatgat actcaatgta cattagttaa aattactata agatatctta
    20941 aatttactta tgttcaattg tttttcctaa agacatagca ttacgttctt gaactaaatt
    21001 ttctattaaa ttattcaatg tccaatcaat agatgtctct gttaccatag ctgtaaaagg
    21061 tctatttatt ctcacttttt ttagcccttc taaaaatgca tttacttgtg tgcttggagt
    21121 gttattaaat attacagact tactcttaat cccattttca cattcatttg atatatttcc
    21181 atctaaaatg tctcttatta tattctctcc atttttatca cttaagatga tatggtctct
    21241 tattccagca aagttactta cgtttttaat taatgataat tcttttttat taaagtttac
    21301 aagcataata cacttcctgt tgtgtatttt atctaggtta ttattattta ttttttcaaa
    21361 tgacatttta tccatccttt catttattta tatactttaa tttaccccat acatttaaaa
    21421 taatcaattt ttatagttaa tttttatatt aatacccaaa ttatatttaa atagttaggc
    21481 tatccaaaac taatatataa aattagaata attagattaa tatctacaaa tcataaccta
    21541 aaggagagat accatgaata cattactaaa caataaattt agcattaaaa acttttcaaa
    21601 tgcttgggtt atagtacttg gttgtatgtt aatacaagct gttccattta gtattgtagc
    21661 aagtatacag cctcaattta taagttatgt tgtagaagga gaaggcttta gtttagcttc
    21721 tttttctcta atattcacta ttggtactat tgcctcagca attgcatcac cttttatagg
    21781 tgtattattt tctaaagtca atattaagat actatattta ttaggttgta taatttctag
    21841 tattgctttt gctacttttt ctatatgtac tgaactatgg caattttaca ctgtatcatg
    21901 tattgttcaa attggcgcta caattatatc tgctatagga atacctgttt taataagtgc
    21961 ttggtttgat gaagaaagca aaggaaaagc tttgggcttt gcttttgctg gtggttctat
    22021 tggtagtata tttctacaac cacttgcagt tcgcttatta gcaactcaag gatacagtcg
    22081 ttcttatcta atatttggaa ttatagcatt aatagttggt atacctgtaa tattatttat
    22141 actcaggatg cctaaagata ttaatgaaaa ggtaaaatct aaaggtaaaa aagagaaagc
    22201 atctgataaa atttctcctt cagtatgggg ttatacatta aaagaagcta aaaaaattaa
    22261 atatttttgg attatgacta ttggatttat atttgttggt atatatgttt catcattatc
    22321 tgtacaatat ccaacttatc ttagaaatta tctaaatcct attctaatag gaacagtagg
    22381 ctccacattt gctttatttg ctctcattgg taatttattt ggaggattat tgtttgataa
    22441 attcggtcaa acaaagccac ttattatatc ttttgtattg gttcttatag caactttatc
    22501 attactattg gtagataaat cttctacttt tgcatttgta tttgccattt tgaaaggtct
    22561 ttctgtattt gcttatatga ttggtcctgc ttatttaact ggctctttct ttggtcaaaa
    22621 ggagtttggt agcatatatg gaatcgtaca attaattttt gcagtgggat ttgcattagg
    22681 ttcaacatta tttggattgt tagttgataa ttttggatac agattttctt ggtctattat
    22741 aattttatca gttataattg cctatacttt acttattata acttctatag gaatgaaaaa
    22801 attgaataaa gaaagattag ataaactaat gcaataattg atttttagat atataaatat
    22861 tataataaaa aagatggagc ttttaatcat aaataaaact ccatcttttt tatattacaa
    22921 tatagctaat tttctcttat ttttaacttt actatctata tcctcatcct taactatagt
    22981 aaatgcctta gttggacaaa catttacaca tgcaggccct tctggcgatt ctatacaaaa
    23041 atcacactta tttgctgtca ttctttctct aaagcaaagc tttccttcat ctacatcttt
    23101 taatgtaact atgtctattg cacctatagg acatgctaac atacaagtct tacatcctat
    23161 acaattttct tcattgattt ttattgtgtt accaacccta actataccac catttggaca
    23221 tgcctttgca caaggagcat cttcacaatg tctacactgt attggagcac ttacttcttt
    23281 tgtctttatg acagaaagtt ttggattaaa atttatttca tctggatttt ggtaaaaaat
    23341 atcttcatct gaatgtgcaa ctacacatcc tatcatacaa gttctacatc ctatgcattt
    23401 atcagggtct gctataacaa aatagttcat cacttagcca cttcctttcc tatacaatat
    23461 gtagtgtgta atagatgatg agatttttct cctaatggtt ctaccagata ttcatcatac
    23521 agttttttaa tatcaggatt ctcatgagat tttctaattg ctaaatgttc atcatgcacg
    23581 tatgttgctt ttgttctatt ttcataagcc aattccttat attcttctag taaaattttt
    23641 ggttgacctc caccacttac acacccaaca ggacaagtca taacttcaat aaaatctaca
    23701 tctaattttc ctttttccaa gtcttcaagc actggtatta cattttttaa tccacttact
    23761 actccaactc ttaagtctaa gtctcctatt ttaatagttg actttctaaa accattttct
    23821 cctcttactt gttttacttc tacatcagga atactttctc cagttataag ctcatatcct
    23881 gttcttatag cagcttccat aaccccacca gtaacaccaa agatagttcc agctccacta
    23941 taactaccta aaggactatc aaatttttct tctcttaaat ttttaaaatc aattcccatg
    24001 tcttttatta aataagctag ttctctagta gttaatacta catctacatc tctatatcca
    24061 ctagaatcca tttcttctct atcacattca tattttttac atgtacatgg cattatagaa
    24121 acactaaata tctttgatgc atctacatta tttatctctg caccataagt tttaaatatt
    24181 gcaccaccca tctgctgagg tgatttacaa gaagataaat gattagttaa tttaggataa
    24241 tttttttcca tgaattttac ccaagctgga caacaagatg taaacattgg aagagttcca
    24301 ccttctgtca ctcttttaat taattcacta ccttcttcca ttatagttaa atctgctgca
    24361 aaattagtat cgtagactct atcaaaattt aaagctctaa gtgctgctgc cattttccca
    24421 gcacctaagt atcctaaata atatccgaaa tcttctccta aagcaactct aactgctggt
    24481 gcacattgga ccatagtgaa tacatttggg tcacttaaag ctttcttcac cttatctata
    24541 tcacatacat tgtatgctgc aaatataggt tcatttatag actctggtat ctctctttct
    24601 tgttttttct cttgtaaaaa ctcaaatcct tcatctataa ctgaggcata agatttacaa
    24661 gtttgaacac attgaccgca cataacacat ctatctaaat ttatctcttg tggctttccc
    24721 tcttcacctt caatagcatt tactggacag acctttgaac attctctaca tccagtacat
    24781 aaatccttat ctatatttat tatcataaac gtcacctacc cctgtatcct ttatttattt
    24841 gttaacaata aactaagtgc agcttgtcta ttcttagctt ttttgtcatc tattggagta
    24901 acaagtttta atgcttcctg aggacatgca cttatacaag ctattttttc attgtcttta
    24961 cataagtcac atttataagc tatcttctta ttttcatcta aaattacttg ttctacttct
    25021 ttaccatctt catattgtgg aagtaagtct atagctccaa aaggacatgc tagcagacaa
    25081 gttttacatc ctatacactt ttcttcattt acagacataa tattgttttc tttaactata
    25141 gctttttgtg gacatgagtt taaacaagga gcatcttcac aatgtctaca ctgtattggc
    25201 atacagaaat tgtcaccttt tactaagtaa agtctaggta taacaggtat atcaactgtt
    25261 ccaactgtat aacccacatt attatgctga ttatgcattg taaaacaagc tacttcacaa
    25321 gctctacaac ctgtacattt gtttgggtct gctattacaa aagagcctat tttatttttc
    25381 atattatccc acctttcaat ttgtatattt ccaaccctct agttattttc atcatatata
    25441 tttatatatt tcactatttt ctcagtactt atatttcgtt tacatttttt gcacaaattg
    25501 tatgtgttat tatatacctt atcagtatca tcaattaata ctccacagat ttcacacttt
    25561 gaatatcttt gctcttcttt tttagtacac atatattcat tctcactttt tgataactct
    25621 ctgtggattt gcataaatat tcatccgtct tcctcttaca aatccaataa gcgttatatt
    25681 taatttttta gaaagctcta ttgataaatt agtcggtgct gattttgata ctacaatagg
    25741 aatttgagtc atagctgctt ttaatatcat ttccaatgat attcttccac ttacagccac
    25801 aattttatct tttaatgtta tgtcatttaa aacacaaaat ccaatagcct tatccattgc
    25861 attatgcctt gctacatctt cacaagtgac tattttttta tcaaaattaa atacagatac
    25921 actatgtacc ccaccagtat ttttaaataa ttctgatgaa tttaaatttt tatccattat
    25981 actgtaaatc gtatcataat ttatttttat attgctcttt acaggagcac atgttatata
    26041 gtctagtgaa tttaaaaata taatttgctc atcttgattt ttttgtttct cttgaatagt
    26101 tacttttgca aaacgttcac tctcatttat ctcaaatttt aataagcttt ttttgttttc
    26161 tatgtatcct tttgttttca agaatcctac tataagttcc tctaaattat atggggtaca
    26221 taaaaatgta tttatatatt tatcatttaa aataaaacta agtggatatt ctgcaattat
    26281 ttcttcatta tctattgata cttcatgttc atttattttg cttacttcta cattaaaaga
    26341 atttaaatat ttgtagtcca ctagactata actattttca aaaactttat ccttactatt
    26401 ttcttgtaat ttagtttttg agtatatatt gttcataaaa tattagcctt tctgcctttt
    26461 ataaatctta aatctaataa atcattagat atttgtagct gtcattttgt ctttcaaact
    26521 ttgatactct tctcttacac atttttctgc taattcttgg tcttcaagtt tttcaagttt
    26581 aacagcacaa tatttatact ctggtgtttt agatataggg tctaaatttg ctattgtaag
    26641 ttcattgcaa gctccaaccc accactgata agtcatatat gttgcacctt ccttaactct
    26701 atcagtaact gttgctcttg ttattacact tcctcttctt gaactaattc taactaattc
    26761 atcatcttca attcctaatt tttcagcatc attagaattt atttgaacac gtcctggttc
    26821 atcttctaat gaactaagtg ttctacaatt accagtcatt gttcttactg aatagtgtcc
    26881 aacttctctt acagtacata aactaaatgg gtattcatca tcttcaactt ccataggggg
    26941 tctccattca gcagcgaaca aattaccttt accattagga gtagaaaact tttgaccttc
    27001 atacagatac atagttcctt tatcttccat actttcactc ttacaaggcc attgaacgca
    27061 tccttgagtt tctatctttt cataagtagc acctaagaaa cttggacata attgtctaag
    27121 ttcgtcccat atttccttag tatttttata gttcatagga tatcccattg ctgtagaaat
    27181 ttcgcttata atttgccagt ctggttttac atctccttgt ggctctatag cttttctcat
    27241 caattggaat cctctatctg cacatgtata tactccctca tgttctcccc aagaagtagc
    27301 tggaagtata acatcagcat gaagtgctgt cttattcata aatatatctt gaacaatgac
    27361 aaattccaat tcatctaatg cttctctaac ttctgatgca tctgggtcac tttgcactgg
    27421 gtcttctcca aatatataat aagctttaag ctttttctct tttagtacta agtctggaac
    27481 ttgagttaaa ctataaccat tattaggaga taattttacc ccccaagctt tttcaaactt
    27541 ttctcttatc ttatcatcag ttacattttg atagccagga tatacattag gaagagctcc
    27601 catatcacaa gctccttgaa cattattttg tccacgtaca ggtcctattc caacgctttc
    27661 tctaccaaaa ttacctgtaa gtagagcaat tgatgcaagt cccttaacta catcaacagc
    27721 ttgaccaaat tgacagacac ccattccata aagtatcata gcttttttac cttttgcata
    27781 ttctctcata gcttttctta taagctcttc tgggattcct gtaatctttt cagcatattt
    27841 agctgtatat ggtttaacaa tttctttata ctcatcaaat ccttgtgtat gattttgaac
    27901 aaattcttta ttgtaaagac cttcttctat taatacattt ccaaaagcat ttactaaaac
    27961 catgtttgtt ccacctttta taggtagatg tatatcagca attcttgcag attcagttac
    28021 tctagggtca gtaacaatta atttagcacc atttttttta gcttttacta ttctatttgc
    28081 cactattgga tgtgaatctg ctccattata tccaaatata aataaaacat ctgcattttc
    28141 tatctctggt atagaattag acattgcacc actacctaat gagtaagcca gaccggctac
    28201 agatggtgca tgtcagacac gagcacaatg gtcaacatta ttagttccta ttgtagctct
    28261 cataaatttt tgcattatat agtttgcttc attaccaggt cctcttgcag aacctgttcc
    28321 cataatagaa tcaggtccat atttttcttt aatttcattt agtcttgaag ctgtatagtt
    28381 tatagcctca tcccattcaa cctcttctag tacaccttgt tttcttatca ttggcttttt
    28441 tagcctaggt gttagaattt tagggtcatt tagaaaatcc catccataac gaccttttaa
    28501 acacaaactt tcttgatttg ttctaccatt agccggttca gctcttaata tcttattatc
    28561 ttttactact agatataatt ggcatccagc tccacagtat ggacaaactg ttaaaatttt
    28621 tttctccata caattccccc cttagattta tcccctgata agtaaaattt atcttaaaga
    28681 taatgagtgt ttttataata tgcaaaatca taaatatttt gcaatactat ctattaaatt
    28741 caacaatttt ctcacaattc ctcttaaatt tcaaaaaaaa tatgtcataa taatctttat
    28801 atttcaacca tttaattaaa atattctagt tcttatgaca tattttttaa tatactcaat
    28861 atatattctt taaacttatt tagttttcac ttaaaacaat atttctgcca attctatttt
    28921 tcattctata gtgagtaatt acagtaaata ttccacttcc aatcagaatc aaatagtaca
    28981 taaatccacc atataaaaat actgcatatc ccatcaaagg ttgagggaat actgatttga
    29041 atatagtgaa aaaggctaat tcattagctc ctacattccc aggtgttggt attggagata
    29101 ttgccatata taaaaatgct tgtaatgtca gtatataaat atagctataa ccttgtaaat
    29161 taaatgcttt atatatccaa aatgaaacac taaagtaagc acttaattga ataaaagtta
    29221 ctattatagt tgaaattaat acctttttat tctttgcaaa aaaatttatt gctttactat
    29281 aatcatctat aaaactttct atggattcga tcttattatc taaaaatttt aaaaacttaa
    29341 tttttgataa atgctttata gtaattctca taaaatattt tattttttta ggatttaaaa
    29401 taactaatat tatcataatt aagacaaatg aattaatggc tatccctaga aacactagag
    29461 gcataatcat tttcatttgt ttttctaaca tagcaaaatt cattaaaaca agtattgtac
    29521 aataaaaagt aactactatt tggaagataa tagacttatt ggtaacaact gcacttgcct
    29581 tactaagtgg cattttacat tttttaagta catatatctg tactggttgg cttcctgaag
    29641 caaaaggagt tattaaatta taataaaatc ccataatggc tagcttaaaa ccaataaatc
    29701 taatattaac tttgtgagtg ctttctataa ttatcttcat tactactcct tcaagcatta
    29761 tgtgacacat tattgccaaa gcacctatga ataaaaactt cttatctacc ataaccataa
    29821 catttgatag cattttaata tccaatgttt taaacactag gtaaattgtt atacctatta
    29881 acaaaaataa aaatgtatac tgtagtaaac tctttctaat attctttagt ctatccatta
    29941 gaaataacca tcctttgctc aagattta
//
LOCUS       DAKBNN010000021        29522 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000021 DAKBNN010000000
VERSION     DAKBNN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.021"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            567..3209
                     /gene="adhE"
                     /locus_tag="OUB02_000931"
                     /gene_synonym="adhC"
     CDS             567..3209
                     /gene="adhE"
                     /locus_tag="OUB02_000931"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCT8884707.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3569..3910)
                     /locus_tag="OUB02_000932"
     CDS             complement(3569..3910)
                     /locus_tag="OUB02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884708.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIYTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIVNIKNQN
                     EKVILNKSIKK"
     gene            complement(3937..4608)
                     /locus_tag="OUB02_000933"
     CDS             complement(3937..4608)
                     /locus_tag="OUB02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884709.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLKERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4620..7196)
                     /locus_tag="OUB02_000934"
     CDS             complement(4620..7196)
                     /locus_tag="OUB02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8884710.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKRGQCEIFSNLNESELNNFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYIAIAMSLFAGIVIYSIYVISIYQRVQEYGILR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIMLIISFFTYLKIRRISPIDAIRKTFGSDKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPNGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDSVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGLI
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7404..8339
                     /locus_tag="OUB02_000935"
     CDS             7404..8339
                     /locus_tag="OUB02_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT8884711.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8358..9095
                     /locus_tag="OUB02_000936"
     CDS             8358..9095
                     /locus_tag="OUB02_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884712.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(9280..14337)
                     /locus_tag="OUB02_000937"
     CDS             complement(9280..14337)
                     /locus_tag="OUB02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCT8884713.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNHENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14362..14781)
                     /locus_tag="OUB02_000938"
     CDS             complement(14362..14781)
                     /locus_tag="OUB02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884714.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(15014..15265)
                     /locus_tag="OUB02_000939"
     CDS             complement(15014..15265)
                     /locus_tag="OUB02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884715.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15354..16085
                     /locus_tag="OUB02_000940"
     CDS             15354..16085
                     /locus_tag="OUB02_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCT8884716.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            16144..17055
                     /locus_tag="OUB02_000941"
     CDS             16144..17055
                     /locus_tag="OUB02_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8884717.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEIFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            17106..17918
                     /locus_tag="OUB02_000942"
     CDS             17106..17918
                     /locus_tag="OUB02_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCT8884718.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            18027..18719
                     /locus_tag="OUB02_000943"
     CDS             18027..18719
                     /locus_tag="OUB02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8884719.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVEEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18806..19747
                     /locus_tag="OUB02_000944"
     CDS             18806..19747
                     /locus_tag="OUB02_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884720.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19784..20941
                     /locus_tag="OUB02_000945"
     CDS             19784..20941
                     /locus_tag="OUB02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCT8884721.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            21093..21653
                     /locus_tag="OUB02_000946"
     CDS             21093..21653
                     /locus_tag="OUB02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCT8884722.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21681..22172
                     /locus_tag="OUB02_000947"
     CDS             21681..22172
                     /locus_tag="OUB02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884723.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22401..22952
                     /locus_tag="OUB02_000948"
     CDS             22401..22952
                     /locus_tag="OUB02_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8884724.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            23038..23970
                     /locus_tag="OUB02_000949"
     CDS             23038..23970
                     /locus_tag="OUB02_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HCT8884725.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIFIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            24024..24887
                     /locus_tag="OUB02_000950"
     CDS             24024..24887
                     /locus_tag="OUB02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCT8884726.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            25134..26996
                     /locus_tag="OUB02_000951"
     CDS             25134..26996
                     /locus_tag="OUB02_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8884727.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26999..28465
                     /locus_tag="OUB02_000952"
     CDS             26999..28465
                     /locus_tag="OUB02_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCT8884728.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICEIYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28489..29325
                     /locus_tag="OUB02_000953"
     CDS             28489..29325
                     /locus_tag="OUB02_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8884729.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10959 a         3527 c         4696 g        10340 t
ORIGIN      
        1 ttatcattaa aaaattaacg gtcttaatat aaaaattatt tatctatatg aaaagcataa
       61 ttattacata ttgacttaaa aataacttat agagattaga ataattttag tttatatatt
      121 ttttaaaact atacagttgt tataaaaaga aaaattgcat acaagtcaaa aataagtgtt
      181 attttataaa aatacgacaa aaattttatt tactacttat acaaataaca tgcaaataac
      241 atataaaatt taataagtaa aaaaaatgaa agatataaat tatcatttta gtattttatt
      301 tagtcgaagt aaattattca aatgaaatat atctcattta gataaaaaat tttagtaaaa
      361 gtaaaaaaaa taatatattt gttaaaaaat aaattgacaa aatattcaga aaaaaacttc
      421 ttgacctaag agagtaaaat aaataaaatc taaggtatag agatgaaatc tataaaatta
      481 ttttaagtat aagttaaaaa tatagatgat gcatttaaga tttataaaaa ttagagatgc
      541 ataattattt tagggaggtt tttagtatgg agaaaaaaga aaaggtagta gaaaaatcaa
      601 atgttgaagt atgtagtcct gaaattgtca acagtgttga aacattaaga atgagattag
      661 aagaaattag attggcacaa aaagaatttg caacatttac gcaagaacag gtagataaga
      721 ttttcttagc tgcttcaact gcagctaatc aacaaagaat acctctagca aaaatggcag
      781 tggaagagac tggtatgggt attgttgaag ataaggttat aaaaaaccat tttgcctcag
      841 aatatatcta taatgcttat aaagatacta aaacttgtgg tgtcatagaa aaagatgaag
      901 catttggttt tacaagaatt gctgagccag taggtgtttt atcagcagtt ataccaacaa
      961 ctaatcctac atcaactgca atattcaaat ccctaatagc actaaaaact agaaatggta
     1021 taattttctc tcctcatcca agagctaaaa actgtactat agaagcagct agagttgtac
     1081 atgatgcagc agttaaggca ggagcaccaa aaggacttat aggttgggta gatgttcctt
     1141 cgattgaatt aactaatgtt gtcatggcag aggcagacct tatattagct actggtggac
     1201 ctggaatggt taaatctgca tactcttcag gtaaaccagc tgtcggagtt gggcctggaa
     1261 atgtacctgc aataatagat gagagtgcag atataaaaat ggctgtaagt tcaatcttag
     1321 tatcaaaatc attcgataat ggtatgatat gtgcttccga gcaagctgta atagtaccag
     1381 aaaaaatata tgaagaagtt aaaaaagaat ttaaatatcg tggagcacat ttcctaaata
     1441 aagaagaaac tgaaaaagta ggaaaagtag taataattga tggttcatta aatgccagaa
     1501 tagttggaca accagcacat gtaatagcca aaatggcgga tgtagaagtt ccaaaaacag
     1561 ctagaataat aattggtgaa gtagaatctg ttgaattaaa tgaacctttt gcacatgaaa
     1621 aactttctcc tgtacttgca atgtacaagt ctaaaagttt tgaagatgca gttgctaaag
     1681 cagaaaaatt agttgcagat ggaggctatg gtcatacatc ttcattatat gcagactcta
     1741 taaatcatcc agagagagta gaaaagtttg taaatgctat gaagacttgt agagtattag
     1801 ttaatactcc atcatcacaa ggtggtatag gtgatttata taactttaaa ttggcaccgt
     1861 ctcttactct tggttgtggt tcttggggtg gaaactcagt atctgaaaac gttggagtaa
     1921 aacatttgct taatatcaag acagtagctg aaaggagaga gaatatgctt tggttcagag
     1981 caccagaaaa ggtttatttc aaaaaaggat gtttaggtgt agcagcaaga gaatttaagg
     2041 atgttatgga taaaaagaaa gcatttatag taacagattc tttcctatat aataatggat
     2101 atactaagaa attaactgat ttattagatg agatgggaat taagcatact acattctttg
     2161 atgtagctcc agacccaaca ttagcttgtg caagagaagg agctaaagct atggctgatt
     2221 tccaaccaga tttaataata gctgtaggtg gaggaagtgc aatggatgct ggtaagataa
     2281 tgtgggtaat gtatgagcat ccagaagtag atttccaaga cttagctatg agatttatgg
     2341 atataagaaa aagagtatat gtattcccta agatgggtga aaaagcgtat tttgctgcta
     2401 tcccaacttc agcaggaact ggttcagaag taactccatt tgcagttata actgaccaag
     2461 attcaggtgt aaaataccca ttagcagatt atgaattaat gccaaacatg gctataattg
     2521 atgctgatat gatgatggag atgccaccaa gattaacagc agcatcaggt gttgatgctt
     2581 taacacatgc attagaagca tatgtatcta tgttaagaac tgaaccagct gacggtctag
     2641 cactacaagc aggtaaaata atatttgaat atttacctcg tgcatataaa aatggaaaga
     2701 atgataaaga agcaagagaa aaaatggcaa tggcatctac tatggcagga atgtcttttg
     2761 caaatgcctt cttaggaata tgtcactctt tagcacataa attaggtgca ttccaccatg
     2821 ttcaacacgg tgttgcaaat gcattattaa taaatgaagt aatcaagttc aattgtgcag
     2881 aagctccaaa taaaatgggt gcattctcac aatacagata tcctgattgc atacaaagat
     2941 atgctgaatt tgcttctttt gcaggtatta aaggaagtac agaccaagaa aaagtagata
     3001 atcttataaa agcaatagat gaactaaagg caaaagtagg tcttcctaag actataaaag
     3061 aggctggagt agaagaatct aaattcttag aaagattgga tgctatggtt gaacaagctt
     3121 ttgatgacca atgtacaggt gctaatccta gatacccatt gatgagtgaa ttaaaagaga
     3181 tatatctaaa agtttattat ggtaaataat atctattgtg caacataggt tctattgggt
     3241 ttgtatataa attattagat ttgaatataa atagagtttt attagagata tgtataaata
     3301 gttataaata atagattata acttaaattc tgaatggaaa tgataaaagg tcagtaattt
     3361 aaagataatt ttgaatatgt gtatttaatc aattaacttt aaaattagtc ggaaaatttt
     3421 tttattacat ataattttaa agcataatat ataaaaaact ttatgaaaaa attataaaaa
     3481 tattcagaaa attctattga ctaatttatt tatcatgata taataagttt agaaaagaaa
     3541 aaaattgggg cctattaagg cccccttact attttttgat agatttattt aaaattactt
     3601 tctcattttg attttttata tttactatag agtcagtata atccacttca aagttggcaa
     3661 aagcagagaa taaatctata tctcttaaca aatgaattgt catacattga ttgttcttat
     3721 ttagccttag aatcaatgct cttacaaatg gaaattgtat tctagaatta aataagttag
     3781 aaccatcagt atatatatct aagctacgat aattaatatc tataaatata tctacatcat
     3841 cattttcttc tcttattatt ctgtagtcaa aatccaagtt taaatttaaa ttacctaaaa
     3901 tcaataacat ttaaatcttc ttttctataa atagttttag agtgttaatt taagctctcc
     3961 atctattata gatataattc tatctgcttt tctagcaata ttttcattat gtgttattat
     4021 tataagggtt tggttatatt tttttataga actttttagt aaatccatga cttcttctgt
     4081 agttttactg tctaaatttc ctgttggttc atctgctagg attatacttg gttttgcaga
     4141 taaagcacgt gcaatagcaa ctctttgctg ttgaccacca gataattggt tgggaagatg
     4201 atttttcctt tccttaagcc ccaataaatt tataaggtct tcaatatatt ctttatctat
     4261 ttttttattg tctaacttta gtggtaattc tatattttcc tttacattta atacaggaat
     4321 taagttatac tgttgaaaga caaatcctac tttttgtctt ctatatcttg ctagttcttt
     4381 atctgaaagg caacatatat ctttaccagc aacttttata gtaccattac ttggtctctc
     4441 aagaccacct actaaatgta gaaatgtact tttcccactt ccacttggcc caattattgc
     4501 aacaaattca cctttattta tttttaaatt tgcattcttt agtgcatgta cagatgtttc
     4561 cttttttcca taaatcttac ctaagttaat agtttccaat ataggcataa ttattcctcc
     4621 tattctgtaa tgtttatagc ttcaactata ctaattttat ttatctttct tgatggaata
     4681 tatgtagcta gaatacctac aattatattt gctacaacaa ctagtatata tattttgtaa
     4741 tctattgaaa atcccaatcc atatgataca aagccctgca tataatacat tttaaattgt
     4801 attataagtc ctgacacgat agtaattata cttgagagta caccatataa tattccttca
     4861 taaagtatca tatttttaaa tcctctctca gagataccaa tagctcttaa cattccaaat
     4921 tcactagttc ttgaagttaa gttataactt atgttgttta ttatgttaag tacactgatt
     4981 atgaacatta ttgctactat gccataacta taaatcatag ttctagatgt catcttatca
     5041 gaattttctt tttccaccat catatctact gttgtagttc caggaacttc acttccaatt
     5101 ttacctaaaa ggttatttat ttttttcttg tttgcatttt cattaaggtc tgcataaact
     5161 aagttatact tatctacacc agttaatttt tttaggtatt cgctactcgt tattacatct
     5221 acactatcat ctccagagta cataccatta tctgcaaaag gataacttat aatagctcca
     5281 actttaaatt catattctaa gtattcataa ttatctttta gactttcata aagccctgta
     5341 tccatcccac catttggata tttgactttt atagtatcac ctacttttat atctacagct
     5401 ggtgtccctt caccaggagt aaaagtcttt gtgtatttct gtgttttaga aatttctggt
     5461 acatatacaa tagctaaatt ttcttttttc atttttttca agtttatatt tccacttact
     5521 aagtattttt ctaatgactt tatcatctca tcattataac cttttagctt ttgctttagt
     5581 gtatatttgc cagtagtctt attttttatg aacagaggat ttagcttata ataaggagtt
     5641 gaatttatag cttcgtagta atctaatcta tttattttat ttacctctat tcttgtgttt
     5701 aagatactag cagttttgac tttgtcaatt ccatctatat tttttatttt atctatctga
     5761 ctatcactaa acccttttat atctttaaaa gaagtattgt taccagtttc attgagataa
     5821 acatttgata gaataaaatc tgcattcata tatgtttgtt cttgtacgga tttgttttgc
     5881 acatctaaaa aagaatattt ataattgtct tttatgacga ttatacctcc tatactcata
     5941 gataatacaa ttatcatgaa ccccttttta tttctaaaga tatttttcgc agatatagat
     6001 tttgttactg acatatttaa agttaatttt gatattaaat aattaacttt ggttacattt
     6061 ttatcagatc caaaagtttt tctgatagca tcaataggag aaattcttct tatttttaag
     6121 tatgtaaaga aacttataat tagcattatt agtattgtac atgcaattga taagagaatt
     6181 attttatctg gaataacaaa aggtgtaaca tttatatttc cttcaaattg aatatttcct
     6241 acagacctat taaatatttg tgcacctccc atacctatac atatacctat aggcattgct
     6301 attagagcaa gtatgaaaag ttcataaagc ataagtttga aaatcctaaa gttggtggaa
     6361 ccaatagctc ttaagatgcc atattcctgt actctttggt atattgatat aacatagata
     6421 ctatatatta caataccagc aaatagactc atagcaattg caatgtattt acttgaatta
     6481 tctaaagtac cgtttttcat agtagattct ataagcattg agtttcttcc tatacttttc
     6541 tcatctaaca tagcattttt tattatattg tctatgtttg tatttatatc actgccttca
     6601 ttaaattcaa tataagttgt aaaattattc agttctgatt cattgagatt agaaaaaatt
     6661 tcacattgtc cccttttttt ctctataaat ctatcttcaa ggattccaac aactttaaaa
     6721 gtttctggat tttctttttg atataattta aaagtaaggt tttgatttat ctctggtttt
     6781 aagcccatac tatttaaaat ccatttctcg actaccacct cattgctagc ttttggaaat
     6841 cgacctttta taagtttaga atgactagtc aaataatttt tatttgcata tagtatgttt
     6901 ataggtaatt tttcattaac atctgtagat gcatagtaag atgttatacc cagattttta
     6961 atatctttgc tagattttat ggactttaat tgatttttct ttatattttt aaagtataca
     7021 tggtcagttc caagctctct tttcaatcta tctacatctg cttgctgagc acttcttgag
     7081 agggtaccaa taccaacaac gagtgatgta gcaagaatta tactcacaac aattgctaaa
     7141 gacctagctt tataactttt tatatacctt agtactattt tcaaattatc tttcatattt
     7201 accacttcct ttcttaacta aatcaattat atatttatct aaaatatata gcaaagtaag
     7261 tattttcaag ttttaagtag ttaattgtac aaattcatct gtatttttac aagtttaatt
     7321 aatatatatg taaattttaa ctttttacat aattttttta aaatgatata atccctatat
     7381 aaattgtaga ggggagggga atcatggata acaattttgt atttcctata gtaatactaa
     7441 ctttgtttat atttagcaat cttataatga ataagtataa attttacaaa gaattagaca
     7501 tattaaaaag aggcatatta acatttttta tatcgtgttt tgcattgatt ttatttatgg
     7561 agggcactaa atatatacaa ttaaggttag gatatagttt tttacttgaa ggagtattaa
     7621 atattatact ttttttggtt ttaattattt taagttttta tacaggcttt aaagttataa
     7681 gattgataaa taataataat gaacaggaat ataaattaca aaaactagat agtaaaataa
     7741 atcataagga taatataatt atcaaattaa gaagtcaaaa acatgactat ttgaaacatt
     7801 tgcaagtagt gtattctcta ttaaatactg ggctagatga agatgccaaa gaatatataa
     7861 tgaatatatc aaatagcttt aaatcaaaaa attcaaggta tggaaaagta tcttatttag
     7921 atgctatagc ttcattaaaa tacgaggaat gtcttgaaaa ggatatatct tttgatgtct
     7981 acattcaaga ttttatggat aatttagaga ttgaacctaa tgatatgagt tcagtactat
     8041 taaacataat agataatgca atagagtctt tgtctactat aaaaaaagaa aagaagtata
     8101 taagattaca tgcatatgaa gatgaatttc agtatattat agctataaaa aataatggtc
     8161 ctaaaataca taatgttgaa agtatatttt ggaatggatt tagtactaaa gaaggacaag
     8221 ataggggata tgggctacat ttagttaaaa atatattaga taagtatgat tatagtatac
     8281 ttgtaaatag tgatgatttt ttaactgaat ttgtaatctt aatcccaaaa tattattagt
     8341 taggaggaga aaagtctatg ataaaagttt tgatttgtga tgatgataag attgtaagag
     8401 atgatatagc aaatatagct aaagagtctg gatatgtaga ttatgttaaa aaagttaagg
     8461 atggtataga ggcaattgaa tttataaaaa atgagaaaat agatttattg attattgaca
     8521 ttgatatgcc atataaaaat gggattgatt ctgctgaaga aattctttct atagataagg
     8581 atatacatat agtatttgtg acgggtttcg ctgattatag tcttgaaagc tttaaagtgc
     8641 atcctataga ttttgtagtt aaaccattta aaaaagaaaa aatacttgat tcaattaaca
     8701 tagcaataga ccatattaac tcccataaga ttgctaataa taattttata gaagacaccc
     8761 tttttgtata taaaataagg aagcagatac atatgattaa ttttgatgat atagttatgt
     8821 ttgagaaaaa ttcaagagca attaatctac acactaaaga tgaagatata ataaagtttt
     8881 atgagaactt tgatgattta aaaaaacgcc tacctcctaa tttctttatg acccatagac
     8941 aatacatagt aaatctaaga tttatacata aggttatacc tataaataaa tcttcattag
     9001 agataaggtt tataaatttt caagaaaatg ccatattaaa caaatctcta gaaaaagatt
     9061 ttttatatag attttatagg gttaaaagat tctagtgaag atagaagaaa aatacttttt
     9121 gtgtgatata ataagtaccg taaagataat gtataagaga agtttatata aaaattattt
     9181 gtaattatga tttaatctat ataaattaca gtatagtata tattggactt tctaattttt
     9241 ttaagaaagt ccaatatatt ttagagatag tttttatact tataaattac ttaagatact
     9301 agggtcttta atcttactat cttcagcaag taatagtatc ttagacataa tctcagcagt
     9361 tttagggtct tcatcagcga atggaaggaa tatcttacct ctactttgag aatgtatagg
     9421 aagtatattc aaagcaccca tccctttttt atgtacaata ccagaaccca tatgcacaga
     9481 atactcacct aaattacctg ctataagagc atgagagcct tcaaaattga cattgttaat
     9541 ttttaataat aataaaagtt ctttagcaat tgctatacgc atctctacag tagaatggct
     9601 agtttcaggg tcaacaccac cagcatgagc gacacttaca acaaggtcta tatcacgcat
     9661 aacttctgag aatagaattg gaggtataga agctaattct aaacttttat aagtttttct
     9721 atcaaaaaat tctactgttt caatagtagg aggctctata tctgctggtg aaaaccaatc
     9781 tgcaagtgca aacatctttg ccactatatt ttctttataa aatactttct gtaatccctc
     9841 ttcataatcg acagtccaaa gctgatttct aagaagtgct acagttttct ttggctgaat
     9901 ttggtgacca gcatatctgc gagatatagt tttttcagat aattcatcac tgtttattct
     9961 ataatactca cggaatactt gcttaaatgg ttgaacaatt tgcttactaa ataaatcatg
    10021 ttggaactca ctccacttac cagattcaaa caaatgaact ggatgtgcaa gttttaattt
    10081 attttttgcc gtcaacttat aagtttcacc ttctatattg caaataaaga acttagactt
    10141 atcattataa acatatccaa tattatcatc acatacccat actagatttt taatcaaagg
    10201 gcagataact ggatttttat agatattggc aatttcttta actgtaaata ctatttcatt
    10261 tttcatagct tgttctaaac tctgttttgc acgagaatac tgactcttta aatctttttg
    10321 gacagattta agttcaagta catatggatg tttagcaata gcttttggta tactctttaa
    10381 agacttgttg cctttttgaa ctttagtaga tgcttttccg ttttcatcaa catctatcca
    10441 aattgaagta ccatcaatat cttttggttt aaatagatga gcaacttcat ttagtttttc
    10501 agtctcaaga gcccacatca tacgatttac atctccaaat ccagcacttc tagctagatt
    10561 ttctagcgct atagtacaag cttttgattc actttcacga cgttgtgcac caaattgctt
    10621 gctttcttta gcaaactttt gaataaattc ataacggaaa gttgcttctt ttaggtctcc
    10681 ttttttcata ggaagtaaag agaaacttct caatttatct tgatttctat ttgtttcaat
    10741 ttctttcatc aacatgtctt tatcaagctt accaagtgta gcatcagcat agagttgtgc
    10801 acgtctatga gagttaccac cactagttat atatttagct gacttgtaaa gtatattgaa
    10861 tctttcttcc ccaatagttt tatatgaatc taaaaaccaa tctacatcaa atgcaccatc
    10921 aatgaaacta cgtggtgaaa taggggagaa tagagcaacc tctgattctt tttgtgcaga
    10981 gaagccttca tttatatgtg catggaagta ccaaattgta cttttcagac ccttccagtt
    11041 taaatatttt tcaattatgt ttacccattg aggggcatac atagcagatt ctatcaatct
    11101 taactcagta atgtcggtat ttttaagtaa actttttaat ttttttgaat catcttcttt
    11161 agtaggatag caatttttaa gtagagaact taaaacatct ttttttgaat aattgctacc
    11221 atatgaatat cctctctgga aagtttcttt accaagagcg accaaaatat ttacaaaata
    11281 atttatacct tcaaagtatt gtatattatt agctagatga gacacttctg ttggcatatc
    11341 acctctacgt gattcaattt ctataattcg gtttgtaaca gtttttaaat tatctttaag
    11401 ccaagggtac ttttctaatg tgtcgccagc ccatctagga tttgtcaatg ttcttattat
    11461 atttgatgcg ttgtatccac ttatcatttc tctattaact tgctcttcag tcattaatcc
    11521 taattcatgt gcacgagcaa acacatttat gctagtatag aaaggaaact tatattctag
    11581 caactgataa tttgaaaacg tagtgtagaa aaacttagta aaagaaatat catcataaat
    11641 aaacgaatgt gctttatcaa gccagtagtt taaaataggg gcatttaaag cagggcagta
    11701 ttcattttgt ctgtaatagt ttttagtaaa ttcaactctt tgttttttac attgttctat
    11761 agtcaataag tttctaagtt tatagtatat ttctaaacaa tagttaaata tttctttatg
    11821 gtcaaactca tttagtaaaa ctcttaaaag ttttcttgta agtccattaa atttaagttt
    11881 ttcaagtttt gtatatattt tattttgttt tttagagtca aggacatttg aatatataga
    11941 tgaaaaccaa cttttttctg tttcattata tccacatgta cttatactat aaaataagat
    12001 atcaagtatt aatttgtaat ctaagtcaag ttttttacta gtttctctcc aaagttctgc
    12061 taatggataa ttatcaagag tatcattaga aactttatat ttaataggag tcaagttata
    12121 agtagaataa cctaaaagta ctttgtctac attacctgac catctttcaa cttcatattc
    12181 ataatctgca ttttcagtaa taagctcatc aaatttttca taaactttta ttattttatt
    12241 tatatccgta tcgagaattt tttttgcttc tttttttgtt gctacagaaa cagaatcctc
    12301 acgagtagat aaacttatat ttgaaataat tttattaagc tttgatgagc tattagaatc
    12361 atctttaaaa tcaaattgtt ctggaatttg aactttgttt tcatggttat atattccaaa
    12421 accattttct ttgcagtaat cattttttgc tttacctaac aaattatcaa ttagtatttg
    12481 aacttgagat gtattctttt tagatttatc aatatcttct ataagtaaaa caagcttatt
    12541 atatagttct tgtctagcag tattgtcttt taataagttt acaatttcaa gacctccaag
    12601 gcatttttga gaatttgaag attttatcag tgaagaagca atattttcca agtttgaatc
    12661 atcttgttca agtaaaattt ttattactgt ttgtctaata gagcttgtct ttaaagacaa
    12721 tagattggtt atcttatcta attctacatc tgttagctca agctttctaa gtagactcaa
    12781 taccgtttct ctattggaaa cactcttatc acttaaacat tccaataaaa attcacgttg
    12841 tctatctgtc tttggttcat ttacaaaatt gactataagt gaagctctta aatctgaact
    12901 cataagatga caattttcca taagcacatc tataaattga gcattcatat cgtatgctgt
    12961 taaagtcatc atacatttaa gtatttcatg agaagaaatt tcaatccaag tccatggaaa
    13021 tacacttcct gaaaatgatt ttgtctttga atttaaaatt tctaaagtat gttttaaact
    13081 ttcaaattgt tcttttctag catcaaagtc tttgtcaata ggagtgtttg acaagtcaaa
    13141 attagtttct ctgttttgcc aactatagta gtagccaaca gaaccttcta catagaaatt
    13201 tgtaattacc catgctagta tctctaaatc atcaccctta atgtttttag atgctataga
    13261 atgtttaaaa tatgtacttt ctgtatgcga taaaaagtaa agagctacaa gttttttata
    13321 tttttcctga caagaaagta agttttcaat aagagagtaa gagtcatata tgttgtaagt
    13381 tgcagtagcc cacaaggcaa aataaatttc aagattatca ttgctattaa ttgattctat
    13441 acggaacttt tcatcagtca atgatttata tgaaagttct aaacatttac gtattattcc
    13501 aggtttttgg tctgaaaaac caagtcctgt ccatgtatcc aaggctctta taacagaact
    13561 aaatcgctct aaattgttgt ccaaaatagt ttttaacatg tatattgatg cagaaattgt
    13621 accctcatct aaactctcta caatttgctg tctaagtcct tcttgttgtt tggctgccaa
    13681 aaggagttta gaaagcattt catagcattt atcactatga ctcttcatga taccaattat
    13741 catttgtctt gtaagtagag cagtttgatt ttctccaaat attacttctt ctaaagcact
    13801 tattactttt tgatttcctt tgtctatttc tagtgctatt ttatcactca ttatagagtc
    13861 atagtacaag accttatttg gaagagtaag ataatctata actgaaaaat cataataaat
    13921 taaatcaaca tagtttgaaa taaaccttat aatacctcta aaataatcca caagtttatt
    13981 tgagcgataa cctttacgat aatatcctcc tgaatatgta taatccatct ttcttttaat
    14041 taattggcaa agttcttcta cttgttcaga agtaaatatt aattctaatg cattttttaa
    14101 aggtccagaa aggagctcgt ccatattttt aatttcgtag tagtggcaat tgtagtattt
    14161 ctttatcata tttaagaatt tgtcttcagg ttctctataa tagtatgaac tattttctat
    14221 gcaatcccaa agagactttg ctaagtcgta gtactgtttt gaagaagatt tagtaaaatt
    14281 atttttaaaa ttttcaatta ttttaggatt tttttctact ctagtacgtt ctaacattta
    14341 tgtacctcca aattatttat tttaaaacat tcttccagca agcaaggtaa gtcttataaa
    14401 agtaaatata tctccacttt tcagatttat agaggaatcg agtttttcaa taacatcttc
    14461 atttataagc actttgtaga ttccatcttc aaatgcctgt aacgctactt ctattgcctt
    14521 ttctacagat tgtttattct cattgtaaat agagccaaat ccaacttttc caatatcagc
    14581 ttgagattca atttcttcat tagttaagaa gtttattatt gatttttctg ggattttctc
    14641 attatattca agaacatttt tagatacaat ttcagagata gcttctttta ggcaattgat
    14701 gttgtcagat acatcaaaag gggtaagttc tagcataggt ttgcgtttgc caacagcctt
    14761 aactctaaaa taaatttgca taaaatcacc tctcaaaata ttaattatgt atttagaaat
    14821 ttaaaattta tctatttgtt atattatatc attttgtgaa ttattaatgt gaattatgga
    14881 attattttac aaaaaataga aaaaaagcta tcttaaagtc taaaaaagca ataaaagata
    14941 acttttaact ataattatat agaattataa ctgtacattt attaaatttt aattttagaa
    15001 ttatttttga taactatctt tttttattta taaattttgc tcctaaatat tttttgatat
    15061 cttcttcagg agaaaagtca tcaataagta tttctttccc agtgtcttca aatactacaa
    15121 gaacttcaca gtcaccttgt aagaaaaatt ttctaactgt atcttcttta acttctctaa
    15181 cttttattac attctttttt tctttttcaa agtcttttgt gtaataactt cccatcatat
    15241 taattatttt tgtatatcta ttcataaaat acctccatat gcgattaaat ttaaaaaata
    15301 tgttataata attgtttgta ttaacttaat aattataaca aggagtaata ataatgacta
    15361 gtataaaaaa taaagatatt ttaaattgta tagattattc cattaaaaat aagctctttg
    15421 acaaactaaa tgatgtatat aagtccttgc cagtaggaaa ttgttctgga tgtggaaatt
    15481 gttgtatgga gtcagtaggt ataaatttaa ttgagttttt gaatatattt tgttatcttg
    15541 aagatagagt tgatttaaaa aaaagctgta taagtaaaat attagattat tattttgagg
    15601 agtatacaag aaagaaccca tgtccattta aagatactaa taatagatgt ttaatatatg
    15661 aagttagacc attaaattgt aggatgtttg gacattggaa aaaagaggat tataataaaa
    15721 atttagataa tgttattgag aaaaataata actatagaga gttaatgaaa aagcaatatg
    15781 gatttgaaat aaatgatgaa gtagtaaatt acaggataga ttattgtgat agatttattc
    15841 caaataaaga ttatttaagt aaatctaaga gattaagctt ttttgatgag cttatgatat
    15901 tagactcaaa tatttattca agtggaaata tagatataga ctttagggat agaggtatag
    15961 ttgaatattt tatagagtca ttactttata gaaatttagc atataatgtg aaaataagga
    16021 tatctaaaga atacaaaata agaaaaagaa ctatagatag aataaaaagg attgttcttg
    16081 tatagtttta tattagtaag taataaattg tatcataaat attgtcaaaa tgaggtgaag
    16141 aaggtgaatc ttacaaaagt aaaaggaaat acctttttta taaaaggtgg cacgaataca
    16201 ggaatatatg tatttgaaga taacacagca cttatgatag acccagggtt atgtggttca
    16261 agacctagga gagtaaataa agtattagat gaaaataata tacaggttag atatattata
    16321 aatactcatg gtcatgatga ccactatgga gcttgtaatc aatttaaaga atattataaa
    16381 gatgtgtgta taatgtcatc tgaatacgat aaattatata tagaaaatcc agaattgttt
    16441 tcaaagtata ttgttggggg aaagagcaat gtatttatgg ataatatctt aaaaaataaa
    16501 atgttggatg atataaaaat agataaatct atagaagctg gaaaaattga attaaacaat
    16561 gaaatatttg atataattga atttcagggt catacacctg gtagtatagc tgttttgacg
    16621 aaggataatg tgatttttgt tggcgattta ttgataggag atgaaatttt atcaaaatac
    16681 gatttcttgt ttctatatga tatacaagaa caaataaatt cattagaaaa attaaaaaat
    16741 atagattttg agtacttagt tttagggcat ggcaagcaag ttatatcgaa agaagattct
    16801 tataaattaa tagaaaaaca tttgaatgct ttaaaaaaat atttatatca aataagagag
    16861 ttgcttgtag aaccgaaatc tttagaaatt ttattaaaaa acattataaa taacaataat
    16921 ttaagcaata attataaaga gtatcatttt tttaaatctt cgttagtatc tgtcataagt
    16981 tacttagttg atttagaaga aatagggtat atattggaaa atggagaatt gctttactat
    17041 acaaaaacaa aataaattat gataaaataa agaaggtcat aaaaaacaaa taaagggtga
    17101 gcaatatgga atacgaaaaa tggaatgaga tactagcacc ttatgaacat gcagtagagg
    17161 aattgaaaat aaaatttaaa aatataagaa aagaatattt agcaaaaggt gaatattctc
    17221 ccatagagtt tgtcacaggt agaacaaaaa aaatatcttc cataatatct aagttaaaaa
    17281 ggataaatgc aaaagatata gaaactgaaa ttgatgatat tgctggtata agaataatgt
    17341 gccagtttgt tgaggatata tatgctatag ttgatttgat aaaagtaaga aatgatatga
    17401 ctataatagg agaaaaagat tatattacaa attacaaaga tagtggttat agaagctatc
    17461 atgttataat aaaataccct ataaactcca ttgcgggttc aaaggaaatt atatgtgaaa
    17521 tacaaataag aactcttgca atgaatttct gggctacaat agaacattca ttaaaatata
    17581 aatatgacca ttacatacct gagactcttg ctgaaaggct tagaagagca tctgatgctg
    17641 cctttctttt agaccaagaa atgagtgaaa taagagagga cataatgaaa gctcaggcta
    17701 tgtaccaaat gaagtctata gctattagag atactctaaa tagaatacaa gagttatatg
    17761 acttgggtga tacacataaa gctatgcagt accaaagaaa acttgataga actgaaacag
    17821 ataaaaatat aactgaaatt ttggaattaa aacaagaaat agatatatta ttagagcaat
    17881 ataaggatac taaggtagat gataaagtag ttcagtaaaa actacttgta gaaaatttgc
    17941 taaatattaa tggggctgcc tcttaatgga aaataatttc taaaaagaga cagcctattt
    18001 agtgtaatta agaaaggaat gatattatga gtttgtatgc tataggtgat ttacattttt
    18061 caacatcagt caataagcca atgaatatct ttggaagtaa ttgggatggt catgaaaaga
    18121 aaattataga taattggaaa gaagttgttg aggaagaaga tatggtctta gtactaggag
    18181 atacatcttg gggaattaat ttatccgaag ctaaaaaaga tttagatata ataagcaaac
    18241 ttccaggtca gaaaatactt ataaagggaa atcatgatta ctggtggaca accgttacaa
    18301 gtttaaataa gctatatgaa gacatgcgtt ttattcagac aaatttttat gaatataaag
    18361 attatgcaat ttgtggaggt aggggctgga tatgtcctaa tgatgttaaa tttgatgaga
    18421 ctgatgaaaa agtttataaa agagaagaac atagacttag actatcatta gaatctgctc
    18481 gtaaaagtgg tcactcaaaa ataattgtga ttactcatta tcctcctacg aatgataaat
    18541 tggaagaatc tttatttaca aagttatttg aagaatataa tgttgaaaag gtgatttatg
    18601 gtcacttgca tggtaaagaa tcttttaaaa tgggattgaa ggggattcga aatggagtag
    18661 aatatacatt agcatcttgt gactatacag aatttaattt aataaaagtt cacgattaaa
    18721 aaacataaat tggtaaatac tataagatac atgattaaaa ataaatattt gagggatttc
    18781 ggttttgaga aaggagacac gacttatgag gtggatatta gtttttatat ttttatatct
    18841 tattccttta gtagtgatat tcagaaatta caagaatttt aagaggtctt gtatctacag
    18901 tagtatttat gtggtattag ctacaacaat aatgataagt aatatttata tgagtggact
    18961 taataaaata aaagagtcta tgtattatca caattatatt tctgatgaga catatgatga
    19021 aaagtatgta tcaaatttta aggaaaaaga aagtactgat ttaaaacagg ataataaaaa
    19081 gaatgaagaa atatcaaata ataaaaattt aaacaacaaa caagatagaa atatacaaaa
    19141 taaagaaagt aatgaagaag gaagtaatga tataaaagaa aagaataatt ctgatagtaa
    19201 tgtttcagtg aataataaat cagaaaaaca agaagataat acaaaagaaa ataataaata
    19261 tacaagtaaa gaaaaaaata gtgaagatat aaaaaaaata gatatggaaa gtataggaat
    19321 ttttaagaaa gaaatttata atatagaaag agcagcttta gttccaatga gagaatgtat
    19381 accttacact aaggatatag caaaaaatat aactaaatta tctagtataa aaaaagatgt
    19441 tacaaatgcc agaaacaagt gtaaagaagt tgtaaaagaa tatgaaaata tgcagattcc
    19501 agaactttct aaggagaagt atacagaaac attgagtaat gctagagatg acgtaaagaa
    19561 agcatatgag cttagagaaa aatctatgga aagtgctttg aaattaataa atactaaaag
    19621 ccctaaatat atagggaaga taacagaata tcttgattta tcagataagc agatagaaag
    19681 ttttaaaaat agattgaacg atttaaaaga agaaataaat aattcagaaa caaataatat
    19741 taattaataa gatttaagga tataagttaa aagggagatt atgatgaata aacagagtat
    19801 aggaaaacta aatatgtttt ttatggggat tactcaaaga aaccttgata atagggattt
    19861 ttttattgag ttagaggtga tttttaaatc tggaagaaaa gagtttaaag gaattgcaat
    19921 agaggaagat aataaatata aatttaattt taaaggaaaa tcagatttat atacgtttaa
    19981 tgagttgctt aagaatattt ctaaagaggc agaaaattat gatggattag tatttaaata
    20041 tgttgagcga ggaactattg ttgtaattga aggagacaat aagaaggtaa atgtaaaata
    20101 tttagacaat aaggaagaag tacctaaaat agatgaattt acggcatctc aaataaaaaa
    20161 cagagattat tatgtaaaag taggacaagc aaatgcactt ttaaaagaaa ttggagtact
    20221 tacaaaagat ggtaagataa aaaatgataa aataagaaaa tacaatcaga tagaccattt
    20281 tgttgaattg atagatagta ttttaaaaga aataaaggat aaagactgta taactatctt
    20341 ggattgtgct tgtggaaaat cttacttaag ctttgtctta aatttttata taaaagaagt
    20401 attgaagaaa aactgttatt ttataggaat agattattca gatgtagtta tagaagcatc
    20461 aaaaaatatg gctaaaaact tgggttataa aaatatgtca tttataaaag aggatttgac
    20521 taattataca cctaataggg atgttgacct tgtaataagt ctacatgctt gtgatacagc
    20581 tacagatatg gcaataggtc taggaattag agcaaaatct gaagctatag tagtagtacc
    20641 atgttgtcat aaggagttat tagggcaata tagatatgaa gctatggaac caatattaaa
    20701 acatggtgta tttaaagcta gatttgcaga tttaataact gatggtttaa gaacattgtt
    20761 attggagggc aatggatatg atacttctgt agtagaatac atatctccac ttgatacacc
    20821 aaaaaattta atgataagag cgataaaaac taaaacgaat aatgacaaag cattaaaaga
    20881 atataaagaa ctgaaatcac aatttggagt ggaaccaacc ttagaaaaac ttatatatta
    20941 gttaaaaatg taaaatttac gaaaagcttg cttaaatatg caagcttttt ggtattatat
    21001 aggtatagtc atatccagag ttttgattta taattaaagc tgtgctataa tatttgatta
    21061 atcaaatcaa taaatgttac atggaggtaa aaatgaacaa attgaatata tgtattgata
    21121 tagatggaac aataacaagc ccttatcatt ttcttccata cctaaatgat atctttaata
    21181 aaaatataac tgaagcagag tgtataacac ataattggga agaattgtat ggttcgggaa
    21241 taagagatgt gtatgcggag tttaataata agtatataca ctcatatgat gaagcaaaga
    21301 ttgtagaggg tgctacagag gtaattagca cattatctaa aggacataat ttatcttttg
    21361 taacggctag acatgagtgt ttaactgatg taactaaaaa ttggctatca agacaaggat
    21421 tttcagatat agatgtttac ttattaggaa gtgattataa agttgaaaag gcaagagaac
    21481 tgagttgtga catatttata gaagataatc ctttaaactc agtgcaacta gctgatgatg
    21541 gtgttaaagt aatacttctg gatactaact ataataaaga tgttaaacat caaaatatag
    21601 ttagagttag taattggata gatattgaaa agattataaa acaaggtatt taaagatttg
    21661 tagattgaaa gggggagcat atgatagatt taaaaattga aaatagaaaa ggaaaaatcc
    21721 atgtaaagag cagtgaaata aaagacattt tagaattacg tccagacttc gaatatgtac
    21781 aagatatatc aaatactata aatcaagaaa atatattggt atttgattgc cagttgacag
    21841 gaaattcttt tgatatggat gatttagata tagaagaaat attggaagag ttaggtgaag
    21901 aaatagatga gtcttatttc agtgttttat ttgaagatgt aagagcgtat ttaaaagatg
    21961 ctactgatga gatagaagca gacttacagg ataattactt atttgataat ttgagatgtt
    22021 attttgatat atacaatata aatcaagaat ttacagattt taagtttgta tttttagttt
    22081 cttttaaaga tataaaaatt tcttcattag caaacctagc taagattgtt tctaagagac
    22141 agcttatagg tgcatctaag ttttatagtt aaaatgaaag attattataa taaaagctag
    22201 taaccttgta aaaatatagg ttactagttt tttatttatt cattttcaca aaatttcaga
    22261 aaactattta cattctaaaa aaagtaatgt atattaaaca taatgtacat tataatggat
    22321 aaaaagtttc tattaaatta ctaagtttat ttaataatca aaataattta tcttatatta
    22381 gtatgtatta gggggtattt atgcaaagtt taaatgaaat aatcgctgaa aatttaaaaa
    22441 agataaggaa agaaaaacat cttagtttag acaagattgc acaacttagt ggtgtaagta
    22501 agagtatgct atctcaaatt gaaaagtgtg aggtaaatcc tactatatct actttgaaga
    22561 aaataactaa tgggctaaaa atctcattta catcattgat ggagaggcag gaaagtgaca
    22621 tagaattgat tcaaaaaagt gatatagacc attttattga agataatgga aagtatataa
    22681 gttatccaat atttccattt gactccaaaa gaagatttga gatatttatg attgaaatag
    22741 aagaaggtgg aaatttagat tcaaatgctg aacttccagg aacacaagag tttataactg
    22801 tttttagtgg cgaagtaacc attaaaataa atggagaaga ttacattgta tctagtggaa
    22861 attctataag atttaaagct gatgtatcac atatatatag aaactcaggc aaaggtatag
    22921 ctaaaatgag tatggtagta tattatatat aaataattta atggaattag agtgatattt
    22981 agatgatttt aggtatgaat atatattata tttaagttat aggagtgtga tttatggatg
    23041 aataataaaa aagcagtttt tttcttttgc agtgatttaa agaaagaccc agtagccagt
    23101 aatgtgataa agtgttgtga agaaataatg gatttaaatc aaactgatat ttttattgat
    23161 gataattttg tattagagtt taaggataaa aacaataact tatttcattt tgtaaaaaca
    23221 aaagatgtaa taagtcataa ttataaatat tatcttccaa ttttaaatag acatttcaga
    23281 gattatgatt ttgcaggagt tgtaaattgg catgagggag aaaatgctcc agaacatatc
    23341 ctaacagctc atacaattgg agatgttcca acaggagaat ttggaaattc aaatccaaga
    23401 tactttaaaa atctaatcaa tgctattgag gatataaaaa atgaaaatct acttgatgaa
    23461 tttactacat taacagaagc aactcactgg tctggaacaa catatggtga agaatctaaa
    23521 ttaattacag aatattctgt acctatgttg gatattgaga taggaagtag tagtgatagt
    23581 tttaataata gtatagcaat acaggtattg gcaaagtcat taattagagt atttgattgt
    23641 gatgaacctt tgaagacttt gttatgtgtt ggaggggtac attttgagaa atctttctca
    23701 gatattataa aaaataaaga atataacata tcaattggac atgtattgcc aaatcaatgg
    23761 attgtaagtg gaatgtatga tgatgaatct ggatttgaga aactagaaaa gtgtataaat
    23821 agtattgaag gaggaattga ttgtatagtt tttcatgata aattaaaagg tacctataaa
    23881 gagcaatgcc gtaaactagg tgaaaaactt aatgtacctg tatttaaaca taaaatatta
    23941 aaaaatccaa aagacttacc tatatggtag ttttatacag ttaatataac aataagtcgg
    24001 attaattttt aagcgaggtc aaaatgatat tatcttattt tgcattatta attagagtag
    24061 tgctattagc ttttgaaaga gtagttgtaa gattattagg tgatgaagaa ggagatattt
    24121 ataaaaatat agcttcatca tttctatttt tctttatagg gggagtatgt ttacttccat
    24181 ttagcataat ggatagagtt agtgattggt cgtttttaat accatgttat ataagtagtt
    24241 tagtctattc aattggttca gtagcgtatg taacgtcttt agcaactggg gaagtttcac
    24301 ttgttactcc aataaatagt ttaaattcat tgtttttact gcttttatca gttatatttt
    24361 taggtgaagg tttaagttta gcaaaagttg caggaatcat aattatgatt ggtggagtgt
    24421 ttatattaaa aaaagtaagc tcttttttaa agaatgtctc tgtaattttt aacaatttac
    24481 catgtagatt aatgttttta tacatatttt tgcagtcagt aggaagagta ttggataagg
    24541 ctttttttgt acaagtatct cctatattat attcaactat actgtatttt ttcgtaggtt
    24601 taaatttatt tatcttttta gcatttaaaa agaaacaaaa aataatttgt gagattttta
    24661 ctaataaaaa gggactttca attttaagtg gtgctattaa tggatattct tatttagcat
    24721 tattaattgc tttaaataat ctagaactta gtatagcaga gccattttct caagtatcta
    24781 tgataataac tttaatactt gctcatttta tattcaaaga aaatataaaa gaaaaaatac
    24841 caggttcaat tttgatacta attggtgggt ggctactgtt actttaattg acataaaaaa
    24901 tgaattataa aggtgctatt agttagtatc agaaaattta aaaaagtatt gacaattctt
    24961 ttaggcaaat gttaatataa taatacaaaa tagtataaat agaagacatg ttactggtaa
    25021 tgcaggcagg atcgaatata ttgctttgat ttttcaaggt gtttatatag gttctgcctt
    25081 ttttgcatct tgaatggcca ttcataaaaa attatggaga aggagattac aagatgaatt
    25141 ataaaaaaac tgccattgac attattgaac aagttggtgg ttcaaaaaat attttagctt
    25201 tagaacactg ttcaactaga ctacgcttta agttgattga tagaagtaaa gtcaatcagg
    25261 atgctttaaa aaaagtacaa ggtgttatga gtatagttaa tggggcacaa tttcaagttg
    25321 tcattggaaa tagtgttgtt gatgtgtatg atgaaatttt aaaagtttgc cctttagata
    25381 caaatgatag tactgtaaaa actgaagaaa aaataaactt aggaacaaga tttatagaat
    25441 ttattattaa tatttttcaa ccactgattt ttgctattgc aggagctggt attttaaaat
    25501 cactgttgat gttattagca atgtttaata ttttaaaatc agattctact atatatacat
    25561 tattagttgc tatttcagat gcaacattct atttcctacc tcttatggta gctgtaacta
    25621 ctgccaatgt attgaaatgt aatcgtttag tagctattgc cgctgtagga tacttattgc
    25681 ttccagcaac aacaactgct ttaagtgaag gtgtcgaact atttggattt acaattccaa
    25741 acattgccta taatgcacag gttttcccag ctattttatg tgtgtctttc ttggcaacaa
    25801 tggagaaaat ttttaacaag tattcaccaa aaccaattcg aactttcttt gttcctatga
    25861 tgtctttggc aattactgtt ccaattactc taactatttt aggtccttta ggatataacg
    25921 ttggtacaat ttttacaacc attattttat tcttgtataa caaaatggga ttcttagttg
    25981 taggattact tgctgctttt cttccattta tggttgcaac aggaatgcac aaagcactaa
    26041 taccttattc aattaataca attggaaagt tgggttatga agctttatat atgacatctt
    26101 cattagctca caatatttct gaaagtggtg cttgttttgc agtagccttg cgaacaaaag
    26161 atgaaacatt aaaacaaaca gcattatctg caggaatttc tgccttgatg ggaattacag
    26221 agccagcttt atatggtatt actttacaac ataaaagagc aatgttaggt gttgtattct
    26281 caagtgcaat ttctggtaca tttttaggat tatttgcagt aaaaggattt gtaattgttg
    26341 gaccagggct tgccagtatg agtatgttta ttgacccaaa caatggaaac aatttgattt
    26401 ttgcaagtat tggttttgtt attgcaatat taggctcgtt tattattaca ctgattattt
    26461 ggaaggaaga tacaaataca gtagagtcat caattggaga tgttgttgaa gaagagctag
    26521 aagaagaaaa agaagtaaat atcttagtaa ctccagtaga aggaaaagta atagatttaa
    26581 gtaaggttaa tgatgaacta tttgcaagta agactttggg agatggagtt gcaattatac
    26641 cgaccaatgg aaatttatac gcaccttgtg attcagaagt tgtgatgtta tttgaaacaa
    26701 aacatgcaat tggtttaaga acaaaaaatg gggctgagat tttgattcat attggaataa
    26761 atactgtaag tatgaatgga gatggattta aaacatttgt taagacaggc gataatgtaa
    26821 aagaaggaga tttattaatt caatttgatt tagacaaaat ctcacatgct aatttagatt
    26881 caactgttat gattgtaaac aataatggag cagattatgc ttataaagtg ttaaaccagt
    26941 cttatggcaa tgtaaaaaaa ggaagtatct tgtttgatgt aaagggagga atataaatat
    27001 gagaaagttt ccagaaggat ttctttgggg aggagctaca gctgctaatc aatttgaagg
    27061 tggttggaat ttaggtggga aagggtggtc tgtatcagat gtagcaaagg cacactttga
    27121 tgcagatgtg aaagattata aatctaataa tgaaatcaca acaaaagata tagaagaagg
    27181 attagcacat cctgaagatg aagtaaatta tccaaaacgt catggtagtg atttttatca
    27241 tcactataaa gaagatattg ctctaatggc agaaatggga tttaagacat atcgaatgag
    27301 tattgcttgg tctcgtattt ttccaaatgg agatgataaa gagccaaatg aagaaggttt
    27361 acagttttat gatgatgtat ttgatgagtt gataagttat ggaattgaac cacttgttac
    27421 tatgtcacat tatgaaccac cattaaatat cgttttaaac tatgatggat ggtattcacg
    27481 acaagttatc aatatgtttg ttaggtatgt agaaactatc tgtgaaatat ataaaaacaa
    27541 agtgaaatat tggcttacat ttaatgaagt tgattctatg attcgtcatc cgtacactac
    27601 tggtggttta gttcgagacc gttttaagga taaaaacttt gaggaagtta tttttcaagc
    27661 tatgcatcat caatttgtag ctagtgccct tgcaacaaaa atttgccatg agattattcc
    27721 aaattcaaaa gtagggtgta tgttaacaaa attaacgtat tatccatata catgtagacc
    27781 agaagatgtt ttggcaactc aacaaaaaat gagaagtatt tatgcatata gtgatacgca
    27841 ggtttttggt gagtatccag tatatttact gtcgtatttt aaaaataata acattcaaat
    27901 agtaaaagaa gaacatgatg atgaaattat gaaaaagtat ccagtagatt ttatttcatt
    27961 ctcttactat atgtcttctt gtgaggcagc tgatacaact ggattagata ttacacctgg
    28021 gaatacgtta ttagctgtaa aaaatcctta tttagaaatg agtgaatggg gttggcaaat
    28081 tgattcaata ggtctacgta tatctttgat tgaattatat gatagataca gaaagccttt
    28141 atttatagtt gaaaatggat taggagctaa agatatttta actgaagata aaaaagttca
    28201 tgaccaatat agaattaatt atttaaaaga acactttaaa tgtatgttag atgcgattat
    28261 cgaagatggc gttgaacttt ggggatacac ttcatggggt tgtattgatt tggtcagtga
    28321 atcaacgaaa caaatgtcaa aacgttatgg atatatttat gtggatgctg atgattatgg
    28381 aaaggggaca tataatcgat ataagaaaga tagtttttat tggtataaaa aagtaattga
    28441 aaataatagt atagatttta aatgaccaat agaaggtgag caaataaaat gaactacata
    28501 ataaagaaag ttttgaactc gagcgttgtt ttagttcatg acacaaagga taatgaattt
    28561 attttgctag ggaaaggaat tggatatggt aaaaaaacag gagaatatat ttgtggttcc
    28621 aatgataatc aaatgtttgt ccctgttgaa aatacaaaat caaaacaatt tttagaatta
    28681 atggatgata ttcctgtcga cattttaaag ataacacagg aaataatagt agaggcagaa
    28741 aagttattga actcatcttt taataaaaac ttatacttaa tattagcaga ccattttaac
    28801 tttgcaattg aaaggatgcg taaaggaata aaaattacaa atcgagtatt ttgggaaata
    28861 aaaaattatt atccaaatga gtttaaggtt ggaatggttg caatttatat ggtagaggag
    28921 agattgggca ttcaattacc agaggaagaa gcagctaaca ttgctttcca ttttgcaaat
    28981 gctatggctt cagatggaaa ttcatatgat acgattaagt atgccaaagt aattggagaa
    29041 ataattaata tttttgtttt ttctttgaat agaaccttag ataaaaagag tatgcattat
    29101 atgagattta taacacatat aaaatttttt gtggaaagat ttttttcaga tagtatgtta
    29161 agcagtggtg atgatttatt atttaatcaa atgaaaaggt caaatccaaa agaaatgtta
    29221 attgcattaa aagttagaga ttttttagaa aagaaatatg gaaaaaagtt aacaaatgag
    29281 gaaatagctt tcttggttgt ccatatcgca cgagttaagc aatagagtga attacagcaa
    29341 tagagtaaat tacagcaata gagtgaatta ctaatattat cttgtacgtc tacatggtag
    29401 aaaattgaaa gaaggtaaag tgtataaaga aactgagaca tattattttg ttgttcatta
    29461 tgtttatttt atgagtgtat cctgttaaaa atttaagtta atatttataa ttaaaatata
    29521 ca
//
LOCUS       DAKBNN010000022        29474 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000022 DAKBNN010000000
VERSION     DAKBNN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.022"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(60..707)
                     /locus_tag="OUB02_000954"
     CDS             complement(60..707)
                     /locus_tag="OUB02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884730.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(700..1863)
                     /locus_tag="OUB02_000955"
     CDS             complement(700..1863)
                     /locus_tag="OUB02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8884731.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            2047..2391
                     /locus_tag="OUB02_000956"
     CDS             2047..2391
                     /locus_tag="OUB02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCT8884732.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            2422..4023
                     /locus_tag="OUB02_000957"
     CDS             2422..4023
                     /locus_tag="OUB02_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCT8884733.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     RKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            4042..5142
                     /gene="dprA"
                     /locus_tag="OUB02_000958"
     CDS             4042..5142
                     /gene="dprA"
                     /locus_tag="OUB02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCT8884734.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            5175..7262
                     /gene="topA"
                     /locus_tag="OUB02_000959"
     CDS             5175..7262
                     /gene="topA"
                     /locus_tag="OUB02_000959"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCT8884735.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            7427..8212
                     /gene="codY"
                     /locus_tag="OUB02_000960"
     CDS             7427..8212
                     /gene="codY"
                     /locus_tag="OUB02_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCT8884736.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            8319..9584
                     /locus_tag="OUB02_000961"
     CDS             8319..9584
                     /locus_tag="OUB02_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCT8884737.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNGDF"
     gene            9615..10385
                     /locus_tag="OUB02_000962"
     CDS             9615..10385
                     /locus_tag="OUB02_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8884738.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNVKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            10494..10940
                     /locus_tag="OUB02_000963"
     CDS             10494..10940
                     /locus_tag="OUB02_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCT8884739.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            10958..12151
                     /gene="nifS"
                     /locus_tag="OUB02_000964"
     CDS             10958..12151
                     /gene="nifS"
                     /locus_tag="OUB02_000964"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HCT8884740.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEESIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            12205..12645
                     /gene="nifU"
                     /locus_tag="OUB02_000965"
     CDS             12205..12645
                     /gene="nifU"
                     /locus_tag="OUB02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HCT8884741.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            12934..14013
                     /gene="mnmA"
                     /locus_tag="OUB02_000966"
     CDS             12934..14013
                     /gene="mnmA"
                     /locus_tag="OUB02_000966"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCT8884742.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    14156..14394
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14462..17101
                     /gene="alaS"
                     /locus_tag="OUB02_000967"
     CDS             14462..17101
                     /gene="alaS"
                     /locus_tag="OUB02_000967"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCT8884743.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            17126..17383
                     /locus_tag="OUB02_000968"
     CDS             17126..17383
                     /locus_tag="OUB02_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCT8884744.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            17393..19564
                     /locus_tag="OUB02_000969"
     CDS             17393..19564
                     /locus_tag="OUB02_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884745.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            19770..20195
                     /gene="ruvX"
                     /locus_tag="OUB02_000970"
     CDS             19770..20195
                     /gene="ruvX"
                     /locus_tag="OUB02_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCT8884746.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            20273..20560
                     /locus_tag="OUB02_000971"
     CDS             20273..20560
                     /locus_tag="OUB02_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCT8884747.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            20842..21300
                     /locus_tag="OUB02_000972"
     CDS             20842..21300
                     /locus_tag="OUB02_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCT8884748.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(21417..22154)
                     /locus_tag="OUB02_000973"
     CDS             complement(21417..22154)
                     /locus_tag="OUB02_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCT8884749.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            22371..24047
                     /locus_tag="OUB02_000974"
     CDS             22371..24047
                     /locus_tag="OUB02_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCT8884750.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            24253..24453
                     /locus_tag="OUB02_000975"
     CDS             24253..24453
                     /locus_tag="OUB02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HCT8884751.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            24507..25670
                     /locus_tag="OUB02_000976"
     CDS             24507..25670
                     /locus_tag="OUB02_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCT8884752.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNTLIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(25881..26303)
                     /locus_tag="OUB02_000977"
     CDS             complement(25881..26303)
                     /locus_tag="OUB02_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HCT8884753.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLKFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(26324..27160)
                     /locus_tag="OUB02_000978"
     CDS             complement(26324..27160)
                     /locus_tag="OUB02_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8884754.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            27458..28054
                     /locus_tag="OUB02_000979"
     CDS             27458..28054
                     /locus_tag="OUB02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HCT8884755.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            28084..28803
                     /locus_tag="OUB02_000980"
     CDS             28084..28803
                     /locus_tag="OUB02_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCT8884756.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            28805..29344
                     /gene="scpB"
                     /locus_tag="OUB02_000981"
     CDS             28805..29344
                     /gene="scpB"
                     /locus_tag="OUB02_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCT8884757.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENKLIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11868 a         3139 c         5176 g         9291 t
ORIGIN      
        1 taattctatt actataagtc actaaaaaaa gtgacttaag tcactttata tttttagatt
       61 tattctatta caattttatt tttgaaagca aagattgcta gttgagttct gtcacgaagt
      121 tctagtttag ctaaaatatt agttatgtta ttctttattg taccttcaga taagaatagt
      181 tcttgactta tttctttatt attaagtcca tttgcaatta gtctaattat agatatttct
      241 ttttctgtta aattgtacat gtcataaaga tgctcttgtt taaccgtttt ttcagaggtc
      301 atgatttttt cagctacact tttatcaagc acaatattgc caaaaaatga agaacgaatc
      361 ccttcatgca atacttcatc agagctatct tttaaaaggt atcctgaagc accaaacttc
      421 atagcatctt ttatatattc atcatcatta aaagttgtta aaattaataa tgttatagaa
      481 ttattatatt cctttataag ccttgtacct agaaccccat cacatacttt cattcgtata
      541 tccataagaa ctatgtctac atcattcatt ttacaaaatt ctaaagcttc atatccatta
      601 gaagcatctc ctacaatttc taaatcatta taagatgata atataatttt aagacctttt
      661 ctaattaatt tttcgtcatc tactataaga actttaattt tattcaaaaa aatcaccccc
      721 tgaaaattta ggaaaagaag ctctaattgt gaaaccatta ttagaattat caaattcaac
      781 tttaccattt aattcatata ttctctcatt gatactatta agtccatttc ctttttttat
      841 attgccacat cctataccat tatcactaat agtaattata agatttgaag tattgaatgt
      901 aataaaaatt ctgatttcag ttgcctttcc atgcctagat gagttggaaa gggattcctg
      961 tatgagtcta tataaagcta tagattgatt tctggataag ttccaagtat tttttgaaat
     1021 agttaattta acattaatat ttgtaagctt aataaagctt ttaactaatt ctttgatttt
     1081 aaataaactt tggtaatttt catattcaac aggttttaac tcagatatag cccttcttac
     1141 atcttgaaaa ctttcagtta caaattctct aagttcatgt actaagtcat aaattagagg
     1201 tttgtctttt aagagttttt ctatagcatt taattgtatt attgttgtag aaagagaatg
     1261 tcctacacta tcatgtattt ctcttgatat tcgatttctt tcttttaaaa ttgctaattc
     1321 ttctatagaa cttgcataag tttccaaatc agcattaact ttttttaatt catctttaga
     1381 aattctaagc ttatcataaa gattttgact gtataatttt gataaattct cattttctat
     1441 atatatagaa agtacagtta taattagcaa tatagagctt gactccagtg ccaaatatat
     1501 atttttatct aaactaataa taagagaaga tattataaca aaagagaaca atatgtactt
     1561 atatttttcc tttattaaaa atgatatatc taatattaaa ggaacaaagt aaatcgaatt
     1621 aaaagttttt gtataattac aaagtataaa aattaatata agttctaaaa agtaagaaat
     1681 aagtataaat ttattctcat ttgataaaga aaaaaatcta acttgattat ttattataat
     1741 tagaaataat attatcatag ttatgaccgg atacaatgta ttttcaacat ttttcaaata
     1801 gcttacaact aatataaata aagataaata tcgtataaaa gcaactaatt tttctctgtt
     1861 cattaaaggc actcctccaa aaaaataaac ttttcctatg aaaactagaa taatatcata
     1921 aaagaataaa gtcaatagaa gtatttatta atattgatgg aaataaaatc aatccaaaaa
     1981 cttgaatagt aaaattgtat actgaccata atttaaaagt gaaaatttaa tgtattggag
     2041 tgaataatga ataataaaga aaaaggtgat tttggagaaa aagtagcagt taattatttg
     2101 ctatcaaaag gagcaaagat actagaaaaa aattacagat taaagatagg ggaaatagat
     2161 ataatagcaa aaatggaaga tgaaattata tttgtggagg tgaaatcaag gtctaacata
     2221 aagtttggct acccttgtga atctgtaagc ttcaaaaaaa gaaaaaagat aattggagtt
     2281 gcaagttatt atattataaa aaataactta aataacactc ccataagatt tgatgttata
     2341 gaggtatatt tattagaaaa aagaataaat cacataatga atgcttttta aaaaagataa
     2401 tgtttttgga aaggattaaa tatgttaagt attattaatt caagcaactt agtaggaatc
     2461 gacagctttt tagttaaggt tgaagtagat gtaagcaatg gaatacctag ttttaatatt
     2521 gttggtcttc caggcaaaga aattaaagaa gcaagagaaa gagtaaagtc agcaattctt
     2581 aatagtggat ataaatttcc aagcacgaga atagttgtaa atttatcacc agcagatata
     2641 aaaaaagaag gagctttttt agatttatct ataagtatag gccttttaag agagttaata
     2701 aaaaaagatg aaaattacat aagagaaagt atgtttatag gagaattatc attagatggg
     2761 aaaataagaa aagtaagagg tatattgccc ataattatgg gtgcaaaaac tcagaacata
     2821 aaacgtattt ttataccaat tgagaacata aaagaaagtc tatttgtaga tgaaattgat
     2881 ataattccta ttaaaagttt aaaagaatgt gtcgattttt taaacgaaga gataaaagtc
     2941 gataaagtaa ggataatgtc ttttttggat gataagtcaa gaaaagagaa cgaagaatta
     3001 gaaaaagata atagttatat agattgcaaa tatactaaga ttaataatga agaaagtaaa
     3061 tatgatgaag actttaaaga tgtaaaagga aattattttg taaagaggag tgcagaaatt
     3121 gctgctgctg gaaatcataa tatgtttatg attggacctc caggctcagg aaaaactatg
     3181 attgcaaaaa gagtaaggac aattttacca gatataagtg tagaagaaat gatagaagta
     3241 agtaaggtct atagtatatt gggtatgata aatgaaacta aaggaataat tgataaaaga
     3301 ccttttagag cacctcatca tacaactaca aagcaatctt taattggagg aggaatggat
     3361 gcaagacctg gagaaattgc tttagcacat agaggtatct tatttttaga tgagatagcc
     3421 gaatttgata gaaaaatact tgagacttta agacaaccta tagaagatgg atatgtaaat
     3481 atatccagag taaagtacag tgcaaaatat ccttgtagag tactgttagt tgcagctatg
     3541 aatccatgtc catgtggtta ttatatgtca gagacagagt gtagatgtag aagtaacgaa
     3601 attgatagat acataaataa aatatcaggt ccattgttag ataggtttga tatttttgta
     3661 gaagttaatt ctataaaata ttcagatttt aattctttaa aacaagaaga aagctctcaa
     3721 aaaataaaga gaagagtaga aaatgctaga aaaatacaga taaatagatt taggaaggat
     3781 aatataaaaa ataatagtga aattaaagct tacaatttat ttaaatattg taaactggaa
     3841 aaagaagctt caaaaacggc tgagatgata tttaataaat acaacctaag cagtagaagt
     3901 tatacaaaac ttttaaaaat ggcaagaaca atagcagatt tagaagaaag agatttgata
     3961 aattcacagt gtattataga agctttttca tttagaaagg cttattatag ttattttaaa
     4021 tagttttttg aggtggattt tatggaaaaa agagatactt atttatggtt aaaatcaatt
     4081 ggtggaataa caacaaaaac aattgaaata atagaaaatg aaatagtaaa tattgaagat
     4141 atatttgatt tttctgaaaa agaaatatat aatttaaaaa atataagctt gaatattaga
     4201 aaaaatatag taaaatatag aggtcacgct tatttagaaa atataaaaga actactttac
     4261 aaaaaagcga ttaaatatat ttgtaaatat gataaagaat atcctgaaaa tttaaagaat
     4321 atatacaacg ctccaaagtt attattctac aaaggtgaca taggtttagt taataataat
     4381 tttaatatag ctatagtagg ttcaagaaaa cctacagcct atggaattaa ttgtgcaaaa
     4441 acaataagtt gtcaactatc ccaatatgga gttaatatag taagtggatt agctatagga
     4501 atagatgcat actcgcacat aggttgtatg agtggaaaat ctaaaactat agcagtactt
     4561 ggttcaggag tagacaatcc tcttccaaaa caaaatttac atttatcaaa taaaatatta
     4621 gaaaatgggg gattattatt atcagagtat aatattaact cgacagtagc cccgtaccat
     4681 ttttctaata gaaatagaat tatcagtggt ttaagtgatg gagtagttgt tgtagaagca
     4741 gcaataaaaa gtggtgcact aatcacagta gattttgctc ttgaacatgg aaaaaatgtt
     4801 tttgcaatac ctggtaatat aaattctcaa atgagtagag gttgtcataa aattataaaa
     4861 gaaggtgcaa aattaattga aaatatagat gatattttaa atgaatataa catatttaat
     4921 attattgata aaaaaataaa tcaaaaatat gataatataa gtctaaatgc caagtctaag
     4981 caaataattg aagctataaa aagagaagga aacttgcata tagatagtat ttgtgattat
     5041 actggtatag aaataaagta tgtaaattca ataatcaatg aattagtact aaatgagtta
     5101 gtagtagaaa tgaataataa aacgtacagt ttaaatgtat aaattaagat taggtagtgg
     5161 gggtgtatgg ttttatggcc aaaacattag tcatcgtaga atcgcctgca aaagctaaaa
     5221 ctatagaaaa atttttaggg aaaagtcatt acacagttaa agcatcagtt ggtcatgtca
     5281 gagatttacc taaaagtaaa ttaggtgtag atatagaaaa taattttgaa cctcaatata
     5341 taaatataag aggaaaaggc gatgtaataa aagaattaaa aaaagaagct aaaaaatcta
     5401 aacaagtata tctagcaact gaccctgata gagaaggtga agctatatca tggcatttag
     5461 ctcatatact aaatttagat gaatctgagg attgtagaat agagtttaat gagataacta
     5521 aggatgcaat taaaaaagca attaaacatc ctagaaatat tgatataaat ttggttgatg
     5581 cacaacaggc aagaagagtg ctagacagac ttttaggtta tcaaataagt cctatattat
     5641 ggcaaaaggt tagaaaaggt cttagtgcag gtagagtgca atctgttaca acaaagttaa
     5701 tttgtgacag agaaaaagaa ataaaggcat ttattccaaa agaatactgg acaatagatg
     5761 ttgaggctaa gacagtaaaa gacaaagata taactttgaa attttatggt aaaaatgatg
     5821 aaaagattga attagaaaat gaagaagctg taaatgaaat actaaaagaa atggaaggta
     5881 aagatttaaa ggttgagaag atagaatcta aatcaagacg taaatcagct ccaaaaccat
     5941 ttacaacaag tatgcttcaa caagaaggag caaataagtt attttttact actaaaaaaa
     6001 caatgatgat agcacaagaa ttatatgaag gtatcgacat agaaggtgaa ggtactgttg
     6061 gtcttatatc atatataaga actgactcta aaagaatatc tgatgaagca aaagaaaaag
     6121 ctaagggcta tatcgtaaac aatttaggag aaaactacta taaaaacact ggtgataaaa
     6181 aagaaaaaaa ggatactaaa aaggttcaag atgcccatga ggcaataaga ccaacctcag
     6241 ttgatagaac tcctgagagt ataaaagcct cattgagcaa agaccagtac aagttataca
     6301 atttaatttg gagaagattt gtagctagtc aaatggaaga ttcagtgttt gatatactaa
     6361 atgtagaatg taaaataggc gatattgtat ttaaggcaac tggttcaaaa atgaaatttg
     6421 atggttatac caaggtatat aatttttcag aaagagaaga caaaatttta ccaagtatag
     6481 aaaaaggaga tatattaaat atagaaaata tattgccaga ccaacatttt acacaaccac
     6541 cagcaagata tacagaagct agtttagtaa aaacactaga agaactaggt ataggtagac
     6601 caagtacgta tgctccaaca atagctacta tactgaatag agagtatgtt gaaaagcaag
     6661 gaacaagtct atgtccaaca gaacttggaa taatagttac tgatattttg gaaaataatt
     6721 tccataaatt tatagatgta gattttactg ctgatatgga aagtaaactt gatgttatag
     6781 aagaaggcaa tacagattgg aaaaagattg tatcagaatc atatgcacca cttaaagagg
     6841 ctattgaaga agctcttgaa aatatagaaa aagtaaatat ggatgaagaa actgatgaga
     6901 tttgcgagaa ctgtgggtct aatatggtta tcaaatatgg tagatttgga aagtttatgg
     6961 cttgtaaaaa ctatcccgat tgtaaaacaa ctagaccaat tatgaataaa attggtgtta
     7021 aatgtccaaa atgtgaagaa ggagatatta tacttagaaa atctaaaaga ggtaaagctt
     7081 tttatgggtg ttctaattat ccaaactgtg actttgtagc ttggaataag ccaacaggag
     7141 agttttgtga gaagtgtggt tcatatatgg ttgaaaaagt gactaaatct gaaacaaaaa
     7201 cagtgtgttc taataaagaa tgtaataaag agacaaaaga gcataatgac ggaaaagagt
     7261 gaaaattgaa aaaaatcaca taattctctt gaaaaaggtt ccaatctatg ataatctttc
     7321 aagtgtaaac aactaataga aaaataaatt ttcattaaat gtgaaaataa tattagaaaa
     7381 tataaaaggt tattaaaagg cctaaatttc atgaggagat gattaaatgg caagtgaagt
     7441 gttacaaaaa acaaggaaaa taaataaaac attacaaaca agtggtggaa gcagtgtctc
     7501 ttttgattta ctggccggag cattgggcga cgttttaagt tctaatgttt atgtagtaag
     7561 tgcaaaaggt aaagtactag gtcttcattt aaatgatgtt caagacagtt cagttataga
     7621 agatgagtat actaagcaaa agaaattttc agatgaatat actcaaaatg tgttaaaaat
     7681 tgatgaaaca ttagaaaatt taaatggtga gaagatatta gaaatctttc ctgaagaaca
     7741 tggaagatta caaaaatata ctacagtagt tccaatatta ggaagcggtc aaagattagg
     7801 aacattggta ctttcaagat attcaaattc attcaatgat gatgatttag taatagctga
     7861 atacagtgca actgttgttg gtcttgaaat attaagagca ataggtgaag aattagaaga
     7921 agaaatgaga aagaaagctg tagttcaaat ggcaataggc actctgtcct actccgagct
     7981 tgaagcagtt gaacatattt ttgctgaatt ggatggaaaa gaaggtctac ttgtagcaag
     8041 taagatagct gatagagttg gtataactag gtctgtaata gtaaatgcac ttagaaaatt
     8101 tgagagtgca ggtgtgatag aatcaagatc attaggtatg aaaggtactc atataagaat
     8161 acttaatgac aaacttacag atgaattaaa aaaattaaaa aacaatcaat aatctatatt
     8221 ttataggttt agattagaaa aggtatctat ttgaaataga tacctttttt agacgaatta
     8281 ttaaggtaat gataagtata ttaaaaagga gtgagaaaat gcctttagca gataaaataa
     8341 gaccaaaaac aatgaatgat gtaataggtc aaagtcatat aataggtaat ggaaaaatat
     8401 tgtctaagat attacaatct aattttttac caaatatgat attttttggt cctcctggtg
     8461 ttggtaaaac tacagtagcg gaaataatag cagaaagaag caataaaagc ttctataaaa
     8521 taaatgctac taattcatct ttagaagata taaaaaaggt gatatcagag cttggaagta
     8581 taaataatgt aaatggagtc ctattatata tagatgaaat acaaagtttt aataaaaaac
     8641 agcaacaatc aatattagaa tttatggaaa atgggagtat aactttgatt gctagcacta
     8701 cagaaaatcc atatcattat gtatataaag ctcttttaag taggtctact gtatttgaat
     8761 ttaaaccttt agaaaaactt gatatagaaa aaggactgaa aagagctgtt gaagtattaa
     8821 atgaagatag ctatatggat attgagtgta ataataatgc tattgaagat atagcaattc
     8881 tttctgatgg agatatgaga agagccttaa atattttaga agtggtagtc tattcaacta
     8941 aaccaaataa ggataatata acttatatag attcagatgt aataagggca tctacattta
     9001 ataaaattat aaactatgac aaaaatggtg atagtcatta tgatatttta agtgcatttc
     9061 aaaaatctat aagaggaagt gacccacagg catctataca ctatctagca agacttataa
     9121 aaggtgggga tttgataagt atatgtagga gacttttagt aatagctagt gaagatatag
     9181 gcttagctta tccaaatgct attgtaattg taaaggcatg tgtagactca gctatgcaac
     9241 ttggatttcc agaagcgaaa ataccattag cagaagctac tattcttttg gctacatctc
     9301 caaagtctaa ttctgcatgt atagcaataa tgaaagctat ggatgaactg gataaagagt
     9361 ttgttaagga tataccaaat agtataaagg atgctcacta ttctggttct aaagaaatag
     9421 gtaggggttg tggatacaaa tatcctcata attttaaaaa tcattatgta aagcaacagt
     9481 atttaccaga tagcataaaa gatagtattt attacatacc acaagaaaat aaaactgaga
     9541 aaaatataaa aaaatattta gaatatctaa atggtgattt ttagactgaa ctttatgtat
     9601 cgggggtaga tattatgatt ataaaactta actcaactta tcacagtaga gttatgaaat
     9661 acttaaaaaa agaacctgag tacaatttgt ttataatagg ggatattgaa agatatggat
     9721 atggaaataa ttttttaaat atatgggcag atgtgggtga acatggtgaa ataaaggcaa
     9781 tattacttaa atattttgaa tttatgatgt tttattctga tggagagtat gatgttgaag
     9841 gtttttataa tctacttcgt aatactaatt acgaagaaat atctgggaaa atatgtgctg
     9901 tagatgcttt agcaaagaga ttaggactta ataatttaaa ggtagttgat ttttgcaaac
     9961 ttcaaactaa aaagtttttg atagataata attgtaatgt aaaggttaag agaattagac
    10021 ttggaaatct aaaaaagaca gtaaaacttt atgacttaat agatgaattt cacagtacaa
    10081 ctttagagaa tttaaaaaat ggactaagga caggtagagg atattgtata gagattaaca
    10141 aacaggtggt gtcaatggca aagagtactt cagaaaatag gactcatgca atgataattg
    10201 gagtaggaac acatcctaaa tatagagcta aaggacttgc tacaaaatgc ctaattaaac
    10261 tatgttcaga gttactgaga gaaaacaaaa ttccttgttt gttttatgat aatgaagagg
    10321 ctggaaagat atataaaaaa ctaggatttg aaaacattgg aaaatgggga atttattcaa
    10381 aataaaaaat taaaattaat cttgacaaaa ttagtagggt ataataaaat tatcttgaaa
    10441 gtaattccta ctaaaacagt atgaattaga tttctatata gaggtgataa tatatgaagt
    10501 tgtctactaa aggtagatat ggactaaaag caatgtttga attagcatta aatcaagaca
    10561 atggaccagt atcattaaaa tttattgcta agaagcaaaa aatatcagat cagtacttag
    10621 aacaaatatt ttcatcttta aaaaaatcgg gtctagtaaa aagtgtgaga ggtgctcaag
    10681 gaggttattt gctttcaaag aatgctgaag acataacagt aggagatata cttgtagtat
    10741 tagaaggacc agttgcatta tctgactgtg tactagatga agatgtatgt gaaaactcaa
    10801 acatgtgtgt aacaaaaata gtttgggaaa agatgaaaaa gggtatagaa gatgttatag
    10861 attcaattac tcttaaagat atgataaatg actataacaa aaataaatta gaaaatgata
    10921 taacaaatat taaaaaatag atttggagat gaatgtaatg gaaaaaagaa gattgtatat
    10981 ggattattct gcaacaacac ctattaaaaa agaagtattg gatgcaatga tgccatacct
    11041 aacagattat tttggtaatg catctagttt tcacacattc ggaagagaag caaaagatgc
    11101 tttggataaa gcaagagaac aagtagcagc ccttataaat gctgaaccta gtgaaatata
    11161 ttttacagca ggtggttcag aaagtgataa ctggacacta gagggtgtag cttatgctaa
    11221 taaaaacaaa ggtaatcata taataacttc aaaaatagaa caccatgcaa tacttcatac
    11281 atgtgaatac ttagcaaaac atcatggatt tgaaataact tatttagatg ttgatagcga
    11341 aggaaaagta gacttaaaac aactagaaga gtcaataaaa gatactacta tacttataag
    11401 tataatgttt gcaaataatg aaataggaac tatacagcct attaaagaaa tatccgaaat
    11461 agctaaaaaa cataagatat tattccacac tgatgcagta caagctacag gtaatattcc
    11521 agtagatgtt aaagaattag gaatagattt gatgagtatg tcttcacata aaatatatgg
    11581 accaaaaggt gtaggagctt tatacataag aaaaggtgta agacttcata actttgtcca
    11641 tggtggagca caagaaaaaa gcaaaagagc tggtacagaa aatatacctg caatagttgg
    11701 atatggaaaa gcagcagaat tagctaagga aaatatgcaa aatcatgtag aaactttaac
    11761 tcgtcttaga aataagttaa tagatggtgt gttagaaaga ataccttaca caagagtaaa
    11821 tggtagctta gaaaatagat tgccaggaaa tgcaaacttt gcattccaat ttatagaagg
    11881 tgaaggaata cttttattat tagatatgtt aggaattgct ggttcaagtg gttctgcatg
    11941 tacttctggt tcattagacc cttcacatgt acttcttgca ataggtttac cacatgaaat
    12001 agcacatggt tcattaagac ttacagttgg agattttaca acagatgatg atatagatta
    12061 tatactagag aacttaccaa aagtaataga aagacttaga agcatgtcac cactttatga
    12121 tgatgcgaag aaacaaggtt tagtaaaata gacataaaga taaagcataa aaaatagaca
    12181 taaattgagt aggaggaaaa gataatgcaa tatagtgata aagttatgga acattttatg
    12241 aatccaagaa atatgggaga aattgataat gcaagtggtg taggtgaagt tggaaaccct
    12301 acatgtggag atataatgaa gatattttta gacatagatg gagatgtaat aaaggatgtt
    12361 aagtttaaaa catttggctg tggttcagct atagcaagtt cttctatggc aactgaaatg
    12421 ataaaaggaa aaactataaa agatgcatta gaacttacaa ataaagctgt tgcagaagct
    12481 ttggatggtt taccaccagt aaagatgcat tgttcagttt tagctgaaca agctgtaaaa
    12541 gcagctctta tagattatgc tcaaaagaac aatatccata taccagaatt ggatggatat
    12601 gttatagatg atgctcatga ccatgatgtt gaagaagaag aataaaaata agtgattgtg
    12661 ttatgagaag tttacaatga ttaaaaagaa gtactaatga tataattagg acataaaaaa
    12721 tagctgtttt taaaatggct attttttatt gcctaaatta agtattttaa cttgatttag
    12781 aggttaatta gttaacaaaa taatccataa gattcaaata ataaagatga ttattttttt
    12841 agaaaataga acaagctata ttttaaataa gtacaatact tgctatatat ggtataataa
    12901 taagtaatgc aaaagatgag gtgaaatttg tttatgaata aaaaagtaat gataggtatg
    12961 agtggaggag ttgatagctc tgtagctgca tatcttttaa aacaacaagg atatgatgtc
    13021 attggtgtta ctatgaagtt gtggcaagat gatgatgttg ttgaaattga aggtggttgt
    13081 tgttctttat ctgctgttga agatgctaga agggtagcaa ataaaatagg tataccattt
    13141 tatgttttaa attttagaga agtgtttaaa gaaaaagtaa tagattattt tatagatgaa
    13201 tatttggaag gaaaaactcc taatccctgt atagcttgta ataaacatat aaaatttgat
    13261 gatttttata agaaggctag acaaataggt tgtgattatg tagctacagg tcactatgca
    13321 aagatagaaa aggatgaaag tacaggaaga tatttgctaa aaaaatctgt tacagataaa
    13381 aaagaccaaa cttatgcact ttataatctt acacaagagc aactagagca tactttattg
    13441 ccaataggtg actatgaaaa agatagagta agagaaattg ctaaggaaat gggtatggca
    13501 gtacacaata agcctgatag tcaagaaatt tgttttgtta aagataatga ctatgcaaat
    13561 tatgtaaaaa aacattctaa aaagcgtatt gaagaaggtt tttttgtaga tactaaaggc
    13621 aatatacttg gaaaacataa gggtatatta tattatacta taggccaaag aaaagggctt
    13681 ggaattactt ttggaaaacc aatgtttgta atagacataa atccaataaa taataccata
    13741 gttcttggag acaatgaaga tttatttaaa aaggaactta ttgctaaaga tgttaacttt
    13801 atatcaatag atacgttaga agaaccatta agagttcaag ccaaaataag atattcagca
    13861 aaaccatcac cagctactat tcatagagta ggagaagata ctattaaaat agtttttgat
    13921 gaagctcaaa gagctattac taaaggtcaa tctgtagtaa tgtatgatgg agatattgta
    13981 gtaggtggag gtataatcga gaaaagttta taaaaacatc taaaaaatta aaaaaataaa
    14041 caataaagta tattaagagt aaataaagtt tataaaataa atttattgta tatgaaaata
    14101 tagtattgat taattttgta aaatttagta atataattag tatattaatt aaaaaacaaa
    14161 tacagtgata aggaatagta aaatatttga tgtcttacag agagagggaa atgctggaag
    14221 cccttagaat atgatatttg aagcagcctt ttagtagcaa tcttgaacaa tttatatttt
    14281 ataaagaaga tttaagtaga gattgacggt agttacgtta tagctaaggt tttccgaaga
    14341 tatcatactt tgtatgataa ttagggtggt accgcgattg tcctcgcccc tataaattta
    14401 tttataggga cgaggttttt tatttaaacg aaaaaataaa atttattagg aggaacatga
    14461 tatggaaaag atgggattaa atgaaataag aagtaagttt ttagaatttt ttgagtcaaa
    14521 aggtcattat gtagcaaata gttattcttt agtaccaaac aatgataaga gtttgttact
    14581 tataaactca ggtatggcac ctttgaaaaa ctacttttca ggtgtagaag tacctccaag
    14641 tgtgagaatg tgtacatctc aaaaatgtat aagaacagga gatatagaaa atgtaggtat
    14701 aactgcaaga catgctacat tctttgaaat gatgggtaac ttctcatttg gagattactt
    14761 taaaagagag tcaatcaaat ggggatggga atttgttaca gaatggctaa atataccaga
    14821 agataagata tgggtgactg tatatgaaga agatgatgat tcttatgata tatgggcaaa
    14881 agaaatgaat ttcccagaag aaagaatggt tagacttgga aaagatgata atttctggga
    14941 aataggtaca ggtccttgtg gtccttgttc agaaatatat tttgatagag gagaagaata
    15001 tggatgtgac aatccagact gtaaaccagg ctgtgattgt gataggtatt tagaattttg
    15061 gaaccatgta tttactcaat ttgatagaga tgaagaagga aattatagtc tattagaaaa
    15121 caagaatata gatacaggta tgggtcttga aagaatgggt tgtattatgc aaggcgtaga
    15181 cactatcttt gaagttgata caattaaatc tatactagaa gcagttgaga agttaacagg
    15241 agtaaaatat ggagagaatc caaaaaatga tatatcaata agaataataa ctgaccatat
    15301 aagagctgta acattcttag ttagtgatgg agtgcttcca tcaaatgaag gaagaggata
    15361 tgttcttaga agacttctta gacgtgctgc tcgtcatggt aagttattag gagtaaaaga
    15421 attattttta cagaaactaa tagatgaagt tataaaagta aatgataaag catatccagt
    15481 tttagttgaa aaagaaagtt atattaaaaa agtagttgga atagaagaag aaaaattcaa
    15541 tgaaactata gaccaaggaa cagaaatatt gaactcttat atagaagtat taaaaaatga
    15601 aggaaagact gtcctaagtg gtcaagaagc ttttaaacta tatgatactt atggcttccc
    15661 tatagactta actaaagaaa tattagaaga agaacatcta tctgtagatg aagaagcttt
    15721 taatgaagaa atggaaaagc aaaaagaaag agctagaaat gctagaggaa atatggatgg
    15781 agaaagttgg aaggaagacc ctctatcaaa attagaatct acagttgaca gtacatttaa
    15841 tgggtatagt gaaatttatg gtgaaggaac tattgaagct atagttaaag atgatgagct
    15901 agttcaaagt gcagaagaag gcgataaggt atctatagta ctagataaca ctactttcta
    15961 tcctgaaggt ggaggacaag ttggagattg tggattgata actaatgaaa acttagtttt
    16021 agaagtatta aatactaaaa aaggtgctaa taatagtata aaacatatag gtataataaa
    16081 atcaggaaga ataagtaatg gggacaaagt aaagacactc gtagatagag aaactagaat
    16141 gtcagctgca agaaatcaca gtgctacaca tttacttcat aaggcattaa gagaagtatt
    16201 aggagaacat gttaatcaag ctggttcttt agtaactcca gaaagactta gatttgatat
    16261 tactcacttt gaagcaatat caaatgaaga attaaaagta atagaagaaa aagtaaataa
    16321 tgttatttta tcatctttag acataaaatg tgatattatg aatataaagg aagctaaaga
    16381 aaaaggagct acagcactat ttggtgaaaa atatggtgat gaggtaagag tagtttctat
    16441 gggagattat tctacagaac tttgtggagg aactcacttg acaaatactt ctcaagtagg
    16501 aatgttcaaa atattatctg aaggtggagt agcagcaggt gtaagaagaa tagaagcaat
    16561 aacaggaaaa gctgtttatg aatacctaaa agaaagagat ggaattatct ctgaagtttg
    16621 tgtaaactta aaatctaaag aagataattt aattcaaaga ataagttctt tattagaaga
    16681 aaataaaaac ttatctaaag aattgcatga tatgaaagct aagatgagtt tacaatcagt
    16741 tgattctatt tttgattcta aagttgaagt aaatggagtt aatttgataa ctaataaatt
    16801 tgaaggtatg gatatggata cactaagaga aacagcagat aatctaagag ataagcttgg
    16861 ttctggtgta gtagtacttg caaatgtagt agatgataaa gttaactttg tagttactgc
    16921 cactaaagat gtattagata agggaataca ttcaggtaat attgttagag aagttgcaaa
    16981 aatagctgga ggaaaaggtg gaggaagacc taatatggca caagcaggag ctagtgatgt
    17041 atctaaggtt gaccaagctt taagctatgc aagtgaggtt attaagacac aagttaaata
    17101 acattcactc aaggaggttt ttagtttgga aaaagattta gactacacta tgaaatttga
    17161 aggaatacca gaagatagga tgagtgtagg agatactatt gattttgtat ataaagcatt
    17221 agttgaaaaa ggatataacc ctataaatca aattataggg tatctccttt ctggtgattc
    17281 tagctatata actagtcata aaaatgcaag agctataatt aaaaaatttg aaagagatga
    17341 gatacttgaa gaagtaatca cgcactatct gaatagaaag taggtcaaat aaatgagaaa
    17401 aaagattatg tcgttgtttg caatagtcat tatgattttt gtatcaactg ctagctatgg
    17461 ttttgctgaa gaaaaaggca aggtcatatt tattgatatg aataggacca atcttggaag
    17521 tatgatgaat attccaatct tgaaaaatga gatagaaaag agaggctatg tagctctcat
    17581 gaatataaga ggggatcaag gtactgatga tagaaggtct tatgcaagta tgggtgcagg
    17641 aacaagagct agtgtaacta ctgaagacta tatcaatttt caaaaagtag accaagatac
    17701 tgctaaagct tttgaagtcg caacaggtca aaaagctaaa gaaataaatg atttaggcat
    17761 taatagatct ataaatgaaa atcttgagta tggagaatat ggagcaaatt tagggtcatt
    17821 aggaaaaact ttatcagaaa atggtttaaa gacatctgta attggaaatg ctgatataat
    17881 tgaaaataat caattagtta aaaacagaaa cttatgttta gtagctatgg accaatatgg
    17941 aaggattgat tcaggtaata ttgataatat taataagaaa gattctaaaa tgccatttgg
    18001 aatatctaca gattatgata aattactaga tgaaactaaa aaatcctata aaaatagtga
    18061 tgctatattt gtagaacttg gagatacata tagattagat atgtatagat taaatctaaa
    18121 tgataatact tatgatacaa tgaagaaaaa tattgtaaaa aatataaata aatatcttga
    18181 acaagtattt tctatggttg gagaaaatga cactgtttat atagcaagtg catttccaag
    18241 tgacctagat tataagaata agagaagact ttctccaata ataaaattta gtggagaagg
    18301 gaaaggtcta ctggaatctg ccacaacaag aaaagctgga attgttgcca atttagatgt
    18361 tggagtagat atactaggta catttggtct tgaaaataaa gagatggtag gtagaagtta
    18421 tactttagta aataaagagg acaatattag ctatctaaaa tcactttatg aaaggatagt
    18481 gtctatatct tcaataaggt caactattat aaatggcttt gtaggcgtaa tatctgcttc
    18541 ttgggtaatt gccatgtttg cgattttctt tagaaattat atgccacata aagagcgtgt
    18601 attcaaagta cttaaggagt ttataaaatt aggcataata atgccactat catttatgtt
    18661 agctcctttg atgaatttta aaactccaat ttcaatatct ttgggaatat ttgcaactac
    18721 gctaatatta tacttgttag gtagaaaatt atttaaggac aatgatataa agcacatggg
    18781 attttttgca acacttacta ttttagtcat agttatagat tctgtatttg gaacatactt
    18841 aatgcaaaat aacataatga gttatgatgc tgtcatagga gctaggtact atggtgtagg
    18901 aaatgaatat gaaggtgtaa ctatagcaag tgcagtattt ggcttagcag tattattaca
    18961 ttataagaag gtatctaaat tacttgttgt aattttttca ttaataatac ttataactag
    19021 tgcatatcct tcaatgggtg caaatgttgg tggagctata tcagaatgtg tagcatattt
    19081 gttgtttata atgcttatat atgacattaa aatagatttt aagaaagctg tattattagc
    19141 tgtttcagct gtagttgtcg tttttgcatt tgcagcttta gatatacttt caggtagtga
    19201 atctcattta ggtatgtttg tacagagaat tttcctaaat ggaccaggag agatattaca
    19261 aacatttgga agaaaaattc aaatgaatat gcaactagca cagacaagtg tatgggtaaa
    19321 tattttactt gtaggaattg gagtaattgc agtgcttata tttaaaccat ctagacactt
    19381 tagaaagata atgaataatt ttccaatctt gtttaaaggg tttattgcat ctatggttgg
    19441 ttgtataatt actcttttag ttaatgattc tggaatagtt gctgcatcta cagcatctat
    19501 atatatactt ataccactaa taataataag tataaatatg ataatatttg aaaaaaataa
    19561 ctaaaaaaat ttttactgtt catataggat ttaaataaaa aatgcaatat gattattaat
    19621 gtaaactgga tgcttacata aaaaatcata ttgcaatttt tatgattgat tatgtaaaat
    19681 tatatgaagc agtaagcctg tgaccttagt tatgagaggt tcagaatatg tatttaatct
    19741 atagaatata aatagataaa ggaggaacta tgttagatgg aagaataatg ggtcttgatg
    19801 ttggtgataa aactattgga gttgctgtga gtgatttgat gggattaact gcacaaggtg
    19861 taaaaacaat aaaaagagtt ggcaagaaaa aagatattga agagctaaaa gcaataataa
    19921 aagaaaaaca agtaaataaa attgtatcag gattaccaaa aaatatgaat ggaactttag
    19981 gtccacaagg agaaaaagta atcaagtttt gtgaattggt aaaagcagaa acaggtattg
    20041 atgtagagtt ttgggatgag aggctttcta cagtagcggc agagaggtct ttattagaag
    20101 cagatgttag tagacaaaaa agaaaaaagg ttatagatat gttggcagca gtaataattt
    20161 tgcaaggata tttagatttt aaaataaatt cataaaagaa ctataaaatt gaatacttat
    20221 gatatataat ataaataata aaaataaaaa taaatttgag gtgaatgaca ttatggaaga
    20281 aaatataata aatttaatag acgaaaatgg cgttgaaagc caatttgaaa taatattaac
    20341 attagaagca gaaggaaaag agtatgcaat attaatgcct ttagatgatg aagaagcaga
    20401 agaagcttta atctttagaa ttgatgagga tgaagaaggt gaaatcttaa tacctttgga
    20461 aagtgatgaa gagtatgaaa ctgttgtagc tgtgtatact gctataatgg aagaagaagg
    20521 tttaaattac gacgaagatg aatcaaatgg acttaactag ataagtaatt aagtttgtat
    20581 ataaaaatca gatatttatt atatttgctt taattaaacc ttattaattg taaaagcatt
    20641 tatagtatat atctgttttt ttgtttttta ttaaaaatac caaaaaattt atgaattttg
    20701 gtgaataata tatcttttat gggtatatat ttatattatg agaataaatt aaatttattt
    20761 aaatgtcttg acaaaaaatg aattatcaat tacaatatta tttaataaag attatcaatt
    20821 gaaaatagta agggtgatgt tatggcaaat acaatggatt tattaaaaga caagttaaaa
    20881 gaaactggtt ttaagattac gccacaaaga agagcaatag ttgaaatatt gcttaaacat
    20941 gaccattcac atctaagtag tgaggaaatc tatgatttag ttagagttga ctgtcctgaa
    21001 ataggcttag ctactgtata tagaacaatg cagctattgg atgaaatagg attaatatca
    21061 aaactaaact tagacgatgg atgtataaga tatgagatta gtctacataa agaagactgt
    21121 cataatcatc atcatcttat ttgtaaaaat tgtggaaaga taatggaagc taaagaagac
    21181 cttttagaca atatagaaaa agagatacaa tcattgtaca aatttaaaat acttgatcat
    21241 gatgttaaat tttatggact atgtgacgag tgtaatggag taagcgattc agaagaataa
    21301 acttctattg cttaatgtcg atggaagaat agtatagaac ttttatattc tatgctattc
    21361 ttccattttt tattataaac catctataaa atgcaaatat acagttttca agatgcttat
    21421 agttttaata ttgaaaatat aattaatcct ataagtacaa aaatgaatga tttttctatg
    21481 aaattactaa atggtcctcc aacagttaaa aaacctaaag aaattttctt ttcgcttagc
    21541 ggataaaaaa ttggtattcc ttgctttgta aataaatctc ctaaaaacat atgacttgca
    21601 taaccaatcg taccataaaa agataaatta ttaatattag tgattaattc tatctgtttt
    21661 agtaaaaaat atactattat agttgcaaat atgctatgag aaaaacttct gtgctttaac
    21721 cagggaaaaa ttgacaacat tagacaaaaa attgtaaaat atatttcaac ttctatgatg
    21781 tataagcata tagctaatag tataaatatt aatgatagaa aaacttttct tagaaatgtg
    21841 tgggttagtt ttgcttcaat aattataatt gcaacaaatg taattattgc gcttaaataa
    21901 aaattatcat ttatatttat tgaaataaag aaaataataa tattaacagc atatataaaa
    21961 attctatata tgtatttgga tacattgttt tttagaatga aatttgagac catagaatta
    22021 gatttatcca aatcgggtag tacagcaaaa gtagctgaaa ctaaaatatc aactaaagat
    22081 ataggaattt taaatattaa agatgtttgg atagctgtta agattcctat tgcacaatga
    22141 gttttaccac gcataaatcc tcctaaatta tccgatatta aatcatataa tatttagagg
    22201 atatcaaaat aaaataggtt tgtcaaaata atacgttaaa tgtattttaa atatagctta
    22261 aaatatgata cgaattatgg tacaataaga ataactggta gcgagtgcgt gatttgatat
    22321 tatttatgat tgtagaacgt gttaaaaaat taaatagaca gaaggagatg atgcaattgt
    22381 tcaaaaagaa caccaacaag ataaaggtaa tggccttggg tggacttaac gaagttggaa
    22441 aaaatatgac tgttgttgag tataaagatg aaataattgt tatagatgcg ggacttagtt
    22501 ttccagagga tgaaatgtta ggagtagata tagttatacc agatataact tatttagtaa
    22561 aaaataggga taaaataaag ggtatattta ttacacatgg acatgaagac catataggag
    22621 ctcttccata tatattaaag aaaataaatg tacctgtata tggggcaaga cttagtatag
    22681 gtcttataca agtaaaactt aaagaacata aaatgaataa tgttaagtta aatgttatag
    22741 gtcctagaca agttataaag cttgataata tggaagtaga gtttttgaaa aataaccata
    22801 gtataccaga tgcttactct attgcaatac acacagacca aggtataata taccacacag
    22861 gagattttaa gattgacttg actcctattg atggagatgt tatggatatg catagaatct
    22921 gtgagttgag caaaaaagga gttcttttaa tgctggcaga tagtactaat gcagaaaaac
    22981 caggatttac tatgtctgaa aagactgttg gggtaggtct tgacgaatta tttgcaaaag
    23041 gaaatggcag aagaattata gttgctacct ttgcatccaa catacacaga ttacaacaaa
    23101 taataaatac tgctgaaaag tttaatagaa aagttgctat atcaggtcgc tcaatggtca
    23161 atgtagttgg agttgcaaaa gaattaggat acctagatat ttctgatgac atgcttatag
    23221 atttaaatga tatatgtaag tatgaagata gtgaattagt gataataaca actggttcac
    23281 aaggtgaacc tatgtcagct cttgctagaa tggctttttc agagcacaaa aaagtagaaa
    23341 taaagagtgg cgatttagtt ataatatctg ctcatccaat accaggaaat gaaaaattaa
    23401 tatcaagagt aatcaatttc ttatttgaaa aaggagcaga agtagtatac agtgatatag
    23461 ctgatataca cgtttctgga catgcctgtc aagaagaatt aaaacttata catgcattag
    23521 ttagaccaaa gttttttatg ccagctcatg gtgaatatag aatgttaaaa agacatgctg
    23581 aaatagctga acagcttggt atggacaaag aaaatatttt tgtcatgcaa acaggagatg
    23641 ttttagaatt agataaaaat tctgctaagg tagcaaaccg tatacaaaca ggaaacatat
    23701 tagttgatgg tctaggagta ggcgatgtag gtaatatagt tttaagggat agaaagcatt
    23761 tatcagaaga tggtcttatg atagttgtag ttactatttc caaggatgag ggtaaggtct
    23821 tagcaggtcc agatataatt tccagaggat tcgtatatgt aagagagtca gaagatttaa
    23881 tggatggagc aaaggatata attaagaatg ttctaaatga atgtgaagag aaaaatatta
    23941 aagaatgggc ttatttaaag aataatatta aagaaaacct aaaagaatat ttatatcaaa
    24001 aaactaaacg aaatccaatg atacttccta tcataatgga agtataatat aaaaaaacta
    24061 taaatttgaa aattagacct cttgtaattt ttaaagtaat atcaaaaatc caattgatat
    24121 tatcttaaga attacaagag gtctttttaa tactcatttt aaaaaaaaat atattacata
    24181 aaataccaat tataacaatc ttttattagt aaaaactaat tatatataaa ttttactgat
    24241 aggaggattc ttatggatga tgtttcaaga caaaatgcta taaaagcttt aaaacaaaca
    24301 aaaatggaaa tagcaggaga gtatggaatg aattatgaag atgcttttga aataatagaa
    24361 aatgcgtcta ataaaggtgt tttagaaggt tactttaaaa agcttgaaaa gaaaaaaaat
    24421 ttaggtcaag gtatatctag gcatttagaa taaaaaaagt taaatgtgag aaaataagtt
    24481 taaataaact ttaagaggtg acatcgatga aaaaattagc tagtactgct ttggctatat
    24541 taattgcgct tactccttta agcttttctt ttgcaaataa taaggaaaat gctgatgcca
    24601 accaactaaa tatatcttca aaatcagcaa ttttgatgga tgttggtagt ggacaaattt
    24661 tatatgaaaa ggatgctcat aaaaagcttc ctcctgctag tgtaactaag gtaatgacta
    24721 tgctacttat agtagaagct ttggattcag gcaagattaa gcttgatgat gaagttcaag
    24781 taagtgaaac tgcatctagt atgggtggta gtcaaatatt cctagagcca ggagaaactc
    24841 aaaaggttga tacattatta aaaggtatag ctgttgcttc tgcaaatgat gcttgtgttg
    24901 ctatggctga acacttagct ggaagtgttg aaggatttgt tgatagaatg aatgctaaag
    24961 caaaagagtt aaatatgaat gatactcatt ttgcaaatac aaatggactt ccagttgcaa
    25021 accactatac atctgctcat gatatagctt taatgtctag agaactatta aaacacgaaa
    25081 tgataagtaa atacttaact acatggatgg ataaggttgt agtagggaag aaacaagtta
    25141 cagtaggtct tgcaaatact aataaactta taaagcatta tcaaggagca actggtgtta
    25201 aaactggttt tactcaagaa gcaaaatact gtttatcagc atctgcaaag agaggtaata
    25261 ctcatctagt tgctgcaact ttaggagcag agacttctcc agaaagattt aatgatgcct
    25321 ctagcctttt aaactatgga tttgctaatt atgaaagtgt taagttatgt agtaaaggtg
    25381 ataatatagc aacacttaca ttggataagg ctgatgaaaa caaagttaaa ttggttgcta
    25441 aagaagattt aaatactctt attaaaaaag gaagttctaa agaatttgag aagaaaattg
    25501 aaatagtaaa aaatcctaag atgccaatta aaaagggaac tgttttagga aaaataaaga
    25561 tatgtaaaga taaaaaagta ataggcgaag tggaattgat aaatactaag gatataaata
    25621 aggcaagcta tctacaaatg cttcaaagaa taattgataa tatgatataa ataattatca
    25681 gatgaaatat aattataata cacaaagata tgattataat acacataaaa aataccctta
    25741 tgtaattatt tgccataggg gtatttttat ttaccatata gaagtatttt ataatcttat
    25801 gatttttata gaaatataga aaagtttatg tatcttaaaa tatcatttga tttaatatca
    25861 tatatagggt tgtttaacaa ttatctaagt aaaggcatac taccagtaag tttattcact
    25921 ttctttttat tcccataaat tgctatccca aaatactgta actcatcttc tggaacacta
    25981 gcaatctttt gtgtaaattc ttcataaaga ttgcaacttt gtgctacatc tgaaaaatct
    26041 acaacagtta aatcgttaaa tttcaaagta tataattttt tacgtaaatc ttttattatt
    26101 tctttgtttc cttttaaaat aggtacagga atgtcaataa ttcctaagtg gctattacct
    26161 gttttgtcaa ttacatctgg gccaacagtt tctggggcat gttttcctag tgtaattccc
    26221 attatagctg ctgtattgga tataattccc attggtaaat tttcgtctat taccatgaca
    26281 cattttgaat ttttattttc catttttatt tctccttttt accttattta aagttgtttg
    26341 aatcatcatt tataaatata ttttgatact gctttggtgt aagaccaata aaattcttaa
    26401 aaaagtttgt aaagtgactt tggtctacaa atcctgtttg tatcgcagca tctataggtt
    26461 ctacgccttt ttctaaaagc tttttagctt tgtttacccg tatattttct aggtatctat
    26521 atggtgtaat accttttaat ttcgtaaaat tacgaagtag agagtactta ttcatacctg
    26581 ctatagtgct taattcgtca agtactatat gttcagcata attattttct agataatcac
    26641 atactgcttg aatttctata tttgtatttt ccaagttaga ttgcaaattt ggctctgtat
    26701 gttcttcaat taattgttca attaggaaga aaaaaagctc ttccttttta aaatccaatt
    26761 cttcttccat gattatatag tgtaattctt gtagtagagg tactagctca ctgcgaaata
    26821 taacaggttg attaaatttt ggtaaataat ttttacctgt aatttcaaag acggtttttt
    26881 tcattatttc tggcttaata ttaatacatc tgtaatcaag tactttatca tctatttgtt
    26941 cacaagtatg actatcaaaa gggttaaaaa gtagcaaatc tccagtactt gtggtatact
    27001 ctttattttt acaagataaa tatctttgac ctttttctat aaaaccaatt acataatgtt
    27061 catgaaaatg gtttgggaat ttttgcatta tgcctttaaa tctatatgct tcaattttta
    27121 attccctatc aaagcatact gtgcgttctt cttgtctcac ttaggtaacc tcctttcatg
    27181 ttatacaaag tgtaacacat atttttttat acttcttgta taatattgac taagagacaa
    27241 taatgtcaat tcatgtcaat ataatattat acttacagta ggtgtaatta ttatattttc
    27301 acaatgaata tgatataata ataaaagtta taaaataaaa agcaaatttt aattttatgc
    27361 ctagaagtta aaattattat aatgtaaaat aattttgatt ataagctatt taattgttat
    27421 aataatatta aaaatttaat ttaggagatg tacattaatg ttaagtatga atataggaga
    27481 aatagtagca agtttagttg gtatagctat ggcaatatca atacatgagt ttggccatgc
    27541 ttattcagct cacttgttgg gagatgatac agctaaagct tatggtagaa tgacattaaa
    27601 tcctgtaaga catgttgacc caataggtct tttggctatg tttatcctta agatagggtg
    27661 ggcaaagcca gtaccagtaa atccaaataa ctttaaaaac tataagatag gaaacctgat
    27721 agtttcactt gcaggcgttt tttgtaatgt tttgactgct atactgtgtg taattatcaa
    27781 taagtatgtg catatgtacg ctataaatac aatagctgga tatgtgcttt tatatagtat
    27841 gggatttgca gcttttaact tacttccgat acctccactt gatggatggg gagttatatc
    27901 tacatttgta ccatataaat ggaatgaata tttgtataaa tttgaaagta tgagttatat
    27961 aattttatta attgctttat ttactggtat atatcaaata atactatcac caatatatac
    28021 agtgttgcta aaaatagtat atttatttgt ttaatataat aaacagatta aagagggatt
    28081 gatatgaagt ataatataca attacaagtc tatgagggac cattagattt actttatgat
    28141 ttaataacaa agcataaaat agatataaag gatatatcta taatagatat aactaagcag
    28201 tatctaaatt atttgaaaat gctagataaa atggatttag agataactag tgaatttata
    28261 actatggcat caaaactttt agagataaag tctaaatatc tgctttataa gcaaaaagat
    28321 gaagaagaag acccaagaat agaacttatg gaaaagttag aagaatatag aaaatttaag
    28381 gtagcatcac aagatataaa agaaaatata acttatgtta atgagagatt ttatagaaat
    28441 aaagaagaga taattattga tgacaatgtg gatttagaag atatatcaat tgaagcaata
    28501 aaaaatatac ttccatacat atttaaagtt aaaacatcac agatagaaaa tgctaatgat
    28561 gagaaactag ataaaatagt aaggaagaaa ataatatctg tagaagaaaa aatattatat
    28621 ataagagata ttataaaaga taagattgaa gttactttta caaatattat aaaatcatat
    28681 gaaaatgatg aaataatagc tactttttta tctatattag agcttataaa agaaaaagaa
    28741 atagtcgtag tacaagatat attttttgat gatattctaa taagaaaaag ttcggagtgt
    28801 taaaatgaaa agagaagata taaagtatat tatagaatct gttatgtttg catatggtga
    28861 accaataagt ataaaagaat taaactatat aataaataaa gaactttcat caaaagaaat
    28921 agagattatg ttaaatttac ttattgagga gtacagagaa caaaatagag gtattcaaat
    28981 aatcaagtta gagaataagt accaaatgtg tacaaataaa gactatgcag aatatataaa
    29041 gaagattatt gaaccaaaga aaaagaaaag tttgagccaa gctacattag aaactcttac
    29101 tataattgcc tacaaacaac caataacaaa ggtagaaata gaggatataa gaggagtaaa
    29161 atgtgataag gtattacaaa ctttgtttga aaataaactt ataagagaag ctggtagatt
    29221 gaataagata gggaaaccaa taatttataa aacaacagat gaatttttaa agttgttgaa
    29281 tatagaaagt cttgaagaat tgccacctat agaaaattat caagaagtgg caacaaatga
    29341 ataaaaatat aaaatcacaa taatttagta tgtaatttta aattttaaaa gatttaaaat
    29401 gctctaagtt gttgtgtttt tttgtataaa tataaaatta taattctctt aaaataaata
    29461 tggataatta agta
//
LOCUS       DAKBNN010000023        28637 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000023 DAKBNN010000000
VERSION     DAKBNN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.023"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(365..2644)
                     /locus_tag="OUB02_000982"
     CDS             complement(365..2644)
                     /locus_tag="OUB02_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8884758.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEVEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2915..4369)
                     /locus_tag="OUB02_000983"
     CDS             complement(2915..4369)
                     /locus_tag="OUB02_000983"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCT8884759.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4533..5402
                     /locus_tag="OUB02_000984"
     CDS             4533..5402
                     /locus_tag="OUB02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8884760.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDANSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5615..7687)
                     /locus_tag="OUB02_000985"
     CDS             complement(5615..7687)
                     /locus_tag="OUB02_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8884761.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(7990..8832)
                     /locus_tag="OUB02_000986"
     CDS             complement(7990..8832)
                     /locus_tag="OUB02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8884762.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(9093..11135)
                     /locus_tag="OUB02_000987"
     CDS             complement(9093..11135)
                     /locus_tag="OUB02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCT8884763.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSIAQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11287..12258)
                     /locus_tag="OUB02_000988"
     CDS             complement(11287..12258)
                     /locus_tag="OUB02_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCT8884764.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(12259..13236)
                     /locus_tag="OUB02_000989"
     CDS             complement(12259..13236)
                     /locus_tag="OUB02_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884765.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            13455..15014
                     /locus_tag="OUB02_000990"
     CDS             13455..15014
                     /locus_tag="OUB02_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8884766.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            15120..16079
                     /locus_tag="OUB02_000991"
     CDS             15120..16079
                     /locus_tag="OUB02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8884767.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGILLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            16082..16894
                     /locus_tag="OUB02_000992"
     CDS             16082..16894
                     /locus_tag="OUB02_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8884768.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(17106..18008)
                     /locus_tag="OUB02_000993"
     CDS             complement(17106..18008)
                     /locus_tag="OUB02_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8884769.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            18363..19541
                     /locus_tag="OUB02_000994"
     CDS             18363..19541
                     /locus_tag="OUB02_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCT8884770.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            19550..20206
                     /locus_tag="OUB02_000995"
     CDS             19550..20206
                     /locus_tag="OUB02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HCT8884771.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            20206..20871
                     /locus_tag="OUB02_000996"
     CDS             20206..20871
                     /locus_tag="OUB02_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HCT8884772.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            20976..21752
                     /locus_tag="OUB02_000997"
     CDS             20976..21752
                     /locus_tag="OUB02_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HCT8884773.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            21814..23148
                     /locus_tag="OUB02_000998"
     CDS             21814..23148
                     /locus_tag="OUB02_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCT8884774.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            23645..24571
                     /locus_tag="OUB02_000999"
     CDS             23645..24571
                     /locus_tag="OUB02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HCT8884775.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(24964..28503)
                     /gene="nifJ"
                     /locus_tag="OUB02_001000"
     CDS             complement(24964..28503)
                     /gene="nifJ"
                     /locus_tag="OUB02_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCT8884776.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         9863 a         4569 c         3741 g        10464 t
ORIGIN      
        1 atttcaaatg atttgtataa attattatga aaaactcttt ttttgatata ataaaattac
       61 ttacatatat aacatttaaa tttataaatt atctttttat tttttaaaat atgtataaca
      121 cacctataga aattactatt attactatct aaagtatttt gctataatcc catatactct
      181 tacctcaaaa acattcaaat agaaaaaata ctacataatt aataaacttt gcccaataat
      241 tcctaaaata atatcataac aatgatttat gttttataat agtatatttt tacataaaaa
      301 agtgaacatt gattaacaaa gttcactttt ttataaattt ttatgttata attcacaatt
      361 ttatttaatt tgtttctttt ctatttaaac tatatctatc taatattaat ttttcatatt
      421 cttctattgg cattggtcta gcaaataaaa atccttgagc catatcacaa cctataaacc
      481 ttaaaaactc tgcttgctct acagtttcaa caccttctga aacaactttt atatcaagtt
      541 gctttgccat ttgaacaata cttgaaacta caatttgact cttttttgca tttcccactg
      601 ttataaagaa ttgcctatct aatttcaata catcaattgg catatcttta agcatattta
      661 aagaagaata accagaacca aaatcatcca tagaaatttt aaatccaaca gatttcaaag
      721 tttgcattat tttaaataat atttttgtat tgtcaaatat tgcactttct gtcaattcaa
      781 tctcaattaa atttggttca acttcatatt cttttgttat tctttcaagc tcagaaataa
      841 agcctggatt ctcaagatgt actcttgata agtttattga tataggaaat gtggggaaat
      901 tttcattttc ccaacgttta aaatttttac aaacttgggt aaatacaaac atatccaatt
      961 ctgttataaa accatttttc tcaaaaatag gaataaatac acctggactt attagacctt
     1021 ttttaggatg ttgccaacgt acaagtgctt cagcacctac aattttagta gttgataaat
     1081 caaccttagg ctgtaaataa actttaaatt gttcattttt taaagcatca tgcatttctt
     1141 gttcaatatc acgttcttct atgagagttt cttttaaatt ttcagcataa atctcataaa
     1201 cttttaaata ttttcttttt actgtagttt ttgcaatcat agctttgtca agacaggtag
     1261 aaatatttgt ttctccctct tcaacaaaat atacacctat agatggaatt aaattgagtt
     1321 ctactcccaa acttgttttt atcattgaca attttttacg tataagttca catatttcta
     1381 taattgactc tctattattt ttcttttcaa atattatagc aaaattgtca tttgaaattc
     1441 ttgaaaatac agattgctct ttaaatatat tcttaattat ctctgctatt tcctttaata
     1501 ctctatctcc ttcttcatac ccaaacctat catttactag cttaaactta tctatatcaa
     1561 aataaattaa aactagattc tttttctcat gagaggataa aaattttccc ccttcaagat
     1621 taaatttatt tgaattgcca atacctgtaa cgttatcttt aaaagcaaat tcttccaatt
     1681 tttccttact ctttttatgt ataaatagta tataaattgt taatgccgta aagattataa
     1741 tcaagactac acatgctgat aatgtaagtt ttataatagc atatgatttc tcagatatag
     1801 cagttttagg aacttcagaa aaaagatacc aatcattgat tcctattggg gcataactta
     1861 aataatatcc ttttccaaac atactataac ttaatgaacc tgatttaaag ttttttatat
     1921 ttgacttcat attattgata ctagattttg atattgaatc acttctgcca atgtaagaaa
     1981 ataaattttc tttagtagag ttggcgtttt tactgcttgg atttaaaaca atatcaccat
     2041 tacttctagt tatataagaa tatccttttc cattaaatgt aggtacagaa agtatttttt
     2101 catacaactt cgtactatgt gtggcaaata taactccttt aacctcatta tctttataaa
     2161 ttggtacaga atatacatta atattactat tggtattttt atcatcttct attacatctt
     2221 ttaattttcc tgttataacc acttctcctt gcattgattt taagaaataa tctcttttag
     2281 agaagtcttc tatataacca tctgtagtgt atgctgtacc atttggtaaa ataactccca
     2341 ttcttttaaa tgaactatta atagcttgtc tttttagtat agaaaggata ttatctactt
     2401 caaaagactc ttctccttca ataaatattg aaattgactc tagtaacttt atatctccat
     2461 taatttgttt ttttaacaca ttgacgcttt gatttgctat ttctgaaaga tattgattcg
     2521 tttcttcacc taatgtctta ttcaaccttg aaccataaat caaacaggaa attgtaatta
     2581 ctaagatact tatgacagca attatggaca aaccatacct attatttttt gcatcactta
     2641 acattatgct tcctgccttt ctcgaatcta tgtatgtata atcattatct tatgacaatt
     2701 attgtaaaca ttttataata atacttattt ttccatattt actgtaatta tacaaaatta
     2761 taaactttat tacaacaata aattaattaa tatagatatt agtgtttata ttattgcatt
     2821 atctatttta caattattta catataaaaa aatgtaatat caaagtgaag cttgatatta
     2881 catttttgtt cttgtatttt gtttaatttt tatgctacat cttacttaaa cactcatttg
     2941 caacttcttt atcatcaaaa tgatgcttaa ctttacctat aatttgataa gtttcatgtc
     3001 ccttacctgt tataattaca acatcatcct tttttgccat acttatagct tctgctatag
     3061 cttgttctct atcaacaaca acatggtaat tttctttttt cttgtcaatt ccttcaagta
     3121 tatcttttat tattgcctct ggttcttcag ttcttggatt atcagaagtt ataatacata
     3181 aatcagaata tttttgtcca attgctccca tcaaaggtct cttttcagta tctctatctc
     3241 caccacaccc aaacacagaa attatttttc cttctgcaaa ttgttgagtt gtctttagta
     3301 cattttcaag tgcgtcagga gtatgagcat agtctacaat tacgcttata cctttatcat
     3361 ttggaactgt ttcaaacctt cctgatactc cattagaaag tctaaggcct tctttatata
     3421 ttggtttagg tatgccaagt acatagcaag cagctataac tgctaaagta ttatatactg
     3481 taaacattcc tggaactggt ataaatatta cttcttcata tgaaggtgtt attaatctat
     3541 aagatactcc atccgaatct gattttatat ctctagccat aaaatcagcc ttagtatcaa
     3601 caccataagt gtaacaaggt acatttatac cttttatatt ttcatatatc ttttttccac
     3661 cttcatcatc tatgtttatt atatttgcca tagtagtctt aaaaaataat ttttcttttg
     3721 cttttctata atcctctaaa tctttatgaa aatctaaatg gtctggtgtt aaatttgtaa
     3781 atatgcctag tttaaagtct gtttcatcaa ctctatttaa tgccaatgaa tgtgatgaaa
     3841 cttccatagc acagtagtca catccattat ccaacatatt attaaaatga tattgtaaat
     3901 ctatactttc tggagttgtt gaattggaaa caatgtcttt ttctccatca aaaattttta
     3961 tagttcctat aagtccaact ttattcttat ttaaagttaa tatctcattt agtatagtag
     4021 ttatactagt ttttccatta gtacctgtaa ctcctataac gttaaatttt tgagatggat
     4081 gattgtaaaa attatcagct acttttgcca tatcttttct tgtatctttt acctttataa
     4141 atgtacatcc ttttatttct acatcttctt caacaagaaa tgcacttgct cccttttcta
     4201 tggcatcttt tatatactta tgaccatctg acacaaatcc ttttatacat ataaataatg
     4261 taccttttgt aacctttctt gaatcatatt gtacattatt aatatctata tttaattcac
     4321 ccttgacatt gattatatcc aacccctgta taatatcatt taatttcata aatgctttcc
     4381 tccctttaaa ctctttctgc taaaattaca ctacatataa tattatatta agaatttttt
     4441 actatgtgaa tggttaaaaa tgcttgaaac attgcaaaaa atgctctata attttctata
     4501 atataactga ggaaaggtct tctataaaaa ctatggataa taaaatcggg tacttaaacg
     4561 ataactttaa aatatttaat attagagata aaaaagatat acaatttgaa taccataatc
     4621 atgattttaa taagataatt atatttataa atggaaatgt aacttatttt gttgatggta
     4681 ttccctataa actaaagcct tgggatatac tctttatcag caataatgaa atacataaac
     4741 cagttataga ttcaaatgtc ttttatgaga gaattgcaat atggattagc ccagaattta
     4801 tgaaaaaaaa tagtgatgct aatagtgact tagaaaaatg ttttaaaata gctacagaga
     4861 ataaatgcaa tagattgcgt ttaaatatac atgatattga atctattaag agttttattg
     4921 agcaaataaa tatttccgaa tccaatactg aatttggaaa tgaaatattg agaaatacat
     4981 tatttataca acttatgata ttcttaaaca gaattttcct aaaaaatgaa aatatggaat
     5041 caacagaaga cgttttttat gatgaaacta ttaagaaagt attaaactat ataaattcaa
     5101 atatagatga taagctttca atagatagca tatcgtctga attttttata agtaagtatt
     5161 atcttatgag aaaatttaaa tctcatactg gaacgtctat acacaattat atagttcaaa
     5221 aaagattaat tttggcaaaa tcactaataa ccaatggtca ccttatgagt gaagtttgta
     5281 ataaatgtgg attcaacgat tattctagtt ttgtaagggc atttaaaaaa aactacggtg
     5341 tatctcctaa aaattatata aacaataaaa atcctttgga tattactctt ttagatgaat
     5401 agataattta tgtaaatttt attttaataa aacttgaagt attagtgtat ttatcttact
     5461 aatacctcaa gttttttaat gttttaaaat atatcaaaat ataatgatat atatttctat
     5521 tacttaaaat atatattaaa taaattgcat gttaactgaa cctatttata tatttattaa
     5581 atctcacata acttttagtt acactatcat atagctatcc tattttttca atatcatctc
     5641 ttaatatact tactctattc tcctcttttg ctgtggttac tcctttttta atgcttaatt
     5701 tctgattttg ttctaaattc gtaaatacta caggacttat tgttgataga ccattttttt
     5761 caatatattc taagtccatt ttaactaatt tttctcccgc ttttacttca tctcctacat
     5821 tcacaaatac ttcaaatcca tttccttcta aatttacagt ttctatccct aaatgtataa
     5881 gtatttctac tccacttttt tgggatttta ttattattgc atgcttagtt ggaaatataa
     5941 ctccaatagt tccatcaact ggtgaataaa tcaatccatc actagattta attgcaaatc
     6001 catcccccat cagtttactt gaaaatacag aatctggcac ttcttctata tctaatatct
     6061 ctccatttgt taatgaatca atcttttcta ctaaagcatt ttccatattt gcaacttcca
     6121 tatttttttt cactttattt ttatcaacat ttttagtaac agtactttca acctttgttt
     6181 ttccctctat tatatctttt atatcagact ttattgaatc cgattttggt ccaaatatag
     6241 cttgaacatt gttgccaata accataacac ctgctgctcc aagtgatttc aatttttctt
     6301 tatctacttt atttatatca tttacaacca atctaagtct tgttatacac gcatctaggc
     6361 tcttcaagtt gtctttattt cctaaagcaa tcaaaatctc tgatgctaat tcattcttag
     6421 acatttcaat gacttcagcg ttagtttctg actcatcttc tcttcctggt gtctttaaat
     6481 taaattttct tatcataaat ctaaatacac tatagtataa tactgcaaaa ccaataccta
     6541 ccaagatagc caagtaccat ggagttctat taggaagaac accaaataat aagaaatcta
     6601 ttaatcctcc tgaaaatgtg aatccaattt taactcctaa taattgcata attgcgtatg
     6661 ataaacctga aaataaacaa tgtattgcaa ataatactgg tgcaacaaat aaaaacgcaa
     6721 actctattgg ctcggttatt cctgttaaaa aagaggttaa tgctgctgat gccattatac
     6781 ctgctacaaa ttttttcttt tctggctttg cttcatgcac catagcaaga gctgctgctg
     6841 gaagaccaaa catcataaat ggaaatgcac ctgtcataaa agtacctgct gtgaaattta
     6901 ctccatcttt aagttgagca aagaatatct tttggtcacc cattacaagc tcaccagctt
     6961 tatttacata ttctccaaat tgataccaaa atggattatg gaaaacatga tgcaaaccaa
     7021 atggtattaa tagtctttcc aaagaacctt ccataaatgc tgctagtgtt tgatttgctt
     7081 ctataacatt ttttgagaaa ctaagtaatc cctcttgtat tggaggccaa ataaaagaca
     7141 atataattcc tattgctata gcaactaatg aagtaacgat tggtacaaat cttttacctg
     7201 caaaaaaacc tagatactga ggtaattcaa ttttataaaa ctttttataa agataagcag
     7261 ccactatccc tataataata ccaccaaata cccctgtttg aattgttggt attccaacaa
     7321 tatttgcata catagaattg tctaccatat cttctgtaac acctgccata acacccatag
     7381 atgtattgag aattaaaaaa cctactatgg ctgccaatgc tgcaacacct tctccttcag
     7441 ttaaaccaac agctacccca actgcaaata taagtggtaa gttattaaat ataatttgac
     7501 ctgaactttc cattatactt attataagtt gaaatgtagg attcgccaaa gctggaaaat
     7561 gagccaacaa tgcgtctcct gctaaagcat tactcacgcc aagtaatata cctgctgcag
     7621 gaagtaatgc aactggtagc attagagatt ttcctacttt ttgtaaaaca ccaaatactt
     7681 ttttcattta cttttctctc cttattattt tatatttttt taaataaaaa agacttaagt
     7741 ttataaaaag ttacattagt atacttaatt ctaaaattaa acttatgtta atataaacaa
     7801 acctatatta acctaatttc agaattaaaa ataaactatt ataatttttt tataaacata
     7861 agtcttgcct gcattatcag taacaatctt aatgttttaa aattattatt ttatattaaa
     7921 ttttaaataa tatattaaaa ttgaacataa tatattaaat ctatacattc tgtattaaac
     7981 tactctttat tataaattca gtacagatat aactcttttg atatgtaatg ttaaataaag
     8041 tagttcatct tgacttacat agtaatcata attcttttct acatgagttt ttattttata
     8101 tgcacaatca taatcctttt catactttac tttaattata tctataattt cattatttgt
     8161 atcattgatt ttttcttttt ttactaatcg ttttgcaaag aattttaaat gagtcagaag
     8221 cctatcataa tttatatcat cttctttaaa ttcaactcta taataatatc ttataatatt
     8281 tagtatgtct tttacaattt tagtaattaa cagtgattcc tttgaacttc ccttatagtt
     8341 tgaatttact atatgcattg ctataaaacc agcttcatct actggaaatt tcacatcaaa
     8401 ttctttattt atataatcta cagcccattt cccaatctca tattcttttt tatgtattct
     8461 ttttatctca tgtagtaaat cattctttat agttatgttt tcatgatatc ttttaattgc
     8521 aaatgcaata tggtcagtaa gtgctacata aatatattca taaagtttag tattcaaatt
     8581 ctcctctgca taagcaatta tttcagtact aacctcaaaa aatttctcat caacctggct
     8641 tgctagttta ctaattttat ctaaaaagct ttcatcttgt attacaaaag ttctttcaat
     8701 tttactttta tctatttctt gacctttctt tttttgaaac ccaattccac atcctgttaa
     8761 tattaattct ttttttatat tttcatctaa aactaataca acattattat tataaatttg
     8821 ttgaataatc ataatcaata tctctcccaa aatacaattt cccttattca taaaaaaata
     8881 cctaaattaa gatacaattc acctgtatac cttaacctag gtattgcctg cttaccagta
     8941 acaatccagc ttgttaacgc ttatatattg tattcaattt atggttttat tctattataa
     9001 ataaatatat atgtcaagta aattattgaa aactatttcc tattttttag tattaatttt
     9061 aacatcaaaa atcaaatttt attccattat atttatgatt ctatcatcat catttcatca
     9121 tagagaatat tttccaataa ctctttagcc acttctcttg atatttgctt agtttcaact
     9181 aattgttgaa cactgtctct ttgactttgt atcgccatta ctctgtagta ttctactctt
     9241 gcctcaaact tatcatcatt ttgaatcttt ctagtaatcc aattcaaata acgattatag
     9301 aatctgattg ctattaaggc ctcatgtctg ttatcttcat cagtattttt atttataaat
     9361 gatatagcag ttttagtaga aagtgcatat gcatggtgaa actctttgat tatttcttta
     9421 ttctcagata ctatttttgt aattttttct tttacttctt tctttacttt tctatttata
     9481 atccacatgc ttatttttaa ttttacaatt ttaaatatag atttttgaac agattttctt
     9541 gattttgtaa tataaaatct atacaattta aaggctctat cagaaatttt accatcttct
     9601 aatagtttca aaacagctaa taatccctgt tggtctgctt ctgcaatcat catacttata
     9661 agtttattat ctggagtatt tagtattcct ttttctgagt ctaccaactg ttcatttaaa
     9721 tcatgtataa ctggttgtac ttcttgtgct ttttcattac taagttttaa aatggcttct
     9781 ttcaaaatta gtttatatgc ctctttatca gataataaag gttctgactc ttgtacgtta
     9841 ttttttaata atcttggcag taaaactgtt gccaatacta aacttataag aataacttct
     9901 gatgatataa ataaaacagt atctctcatt ggaaatacat ctttatcagc caaataaaaa
     9961 ggtatagata aggcagttgc tagtgtaaat gtaccatgaa caccacaagt agcaactatt
    10021 agtgagtatt tacatttaga aatactctct tttacaactt cttcatcctt aaaacctaaa
    10081 aaacctacaa aaaaattatt taaaggattc tttttaggtt ttataaaaga attatgccat
    10141 aaatataccc atataaatct tataacaaat aaaataagta caattaaagt agaaataaaa
    10201 gtcaattcca atgccatatt ttcattttta ctactaagtc ctgtatatat acttggtaac
    10261 aaaaaaccaa gcagtgtaaa gacaaatcca ttcaatacat actccaaaac ataccaagta
    10321 ttgtctgata ttatccttaa tttagtagtt gtattttgta aatgctcttt ttcaattcca
    10381 tgagctatcc ctgccattac aacagctagg attccagata ctcctatttc ttctgctaca
    10441 aaatatacaa aaagtggagt cattatttgt ataactatta ccattggaat ttcttccaaa
    10501 ttccacttgt gtaaactaag tcttattttt ataattatgt atcctaaaat acttcctact
    10561 attattcctc caaaagctgt aatcataaat tgaatccctg cctcttctat tgaaaaaact
    10621 cctgtaactg tggcaagtac tgcaacctta aatgctacta ctccagcagc atcatttaat
    10681 aaagattcac cttcaagtat actcatcata tttttaggaa aatttaatcc ttgtgcaatt
    10741 gattttactg ctactaaatc agttggagat attactgctg ccaaagcaaa tgaaacagat
    10801 aatggcatcc caggaataag aaaatgtata aagattccac ctgcaaacac ggttattaaa
    10861 actaaaccaa atgcaagaag caaaattggt tttctgagct ctaatagctc ttttctcgat
    10921 acattttgtc cttcactgaa tagtaatgga gctatgatac atattacaaa tatttctgga
    10981 ttaaatgaaa atgagaattt aaatggcaaa atagacaata taatccctag tacaatttga
    11041 tatattgcaa gaggaatatt actccacttt ttgctaaata cagttgaaat cgttgtacaa
    11101 accaataatc ccaacaatgt tactaaaatt gacataacta cctctctttt caataataat
    11161 attatagatt aaacttatat atcaactaac ttaatattaa cagcatgaaa tcccatctta
    11221 atcaaaaaaa ttttcagttt attctattat tcttattata tacatcaaat ttaatttttg
    11281 aaatatctat actagatgac atgcaacaaa atgatttctt gatatttctt ttaaaactgg
    11341 aatctctttt ttgcatattt ccttacaata agggcatctt gtatgaaact tacatccact
    11401 tggtggattt gttggagatg gtatgtctcc ttttaatata attctatttt tcttaattgt
    11461 agggtcttgt attggtactg cgctcataag cgcttttgta tatggatgtt gagaatgtaa
    11521 gtaaagttct cctttagttg caatttctat tatagaacct agatacataa cacaaactct
    11581 atgtgcaata tgatttacta cactcaaatc atgagatata aataaatatg aaagtttata
    11641 tttatcctgt aaatccataa gtaaatttat aatttgagat tgaatagaaa catctagtgc
    11701 agaaacaggt tcatcacaaa ctataaattc tggatttaat gctaatgctc ttgctatacc
    11761 tattctctgt ctttgacctc cagaaaactc acctggtttt ctatttaaat ggtacttaga
    11821 aagaccacaa ctttctacaa catttaatat attatctttt atttcattct ttggcactaa
    11881 attatgttct ataactgcct cggatattat ttgttccaca tttaatcttg gatttaatga
    11941 actatatggg tcttgaaata tcatttgcat tttgggtctt aactttctaa gttcttcctt
    12001 ctctaactta tatatatcta caccattaaa ttcaacttta ccttcagttt tttcaattaa
    12061 tctaagaatg gttcttcctg cagttgattt tccacatccc gattctccaa ctatagccaa
    12121 agtttctcct ttttttaaat caaaagaaat tccatctact gcctttacac atttaatttc
    12181 cttatcaaat aatccagatt taacttcaaa gtacttttta agaccctcta cttttaataa
    12241 actctcttct tttttcaatt atatctcctc ctttttatat aaccaacatg caatactatg
    12301 accactattc aattctatta aaggaggtat ctgagttcta catttatcac atactttttc
    12361 acatctatca ctaaaataac atgaatctgg catcccaatt ggatttggta cttgtcctgg
    12421 aatcgaatac aatcttttat ctttgttttt tccaagagca ggctttgatt ttagaagtcc
    12481 aatagtataa ggatgttttg gatttttaaa taattcaaca acctctgcca cttctataat
    12541 tttgccagca tacatcacta atactttgtc acacatttct gcaactactc ccaaatcatg
    12601 tgtaatcatc attatagatg tgtttaattt ttcttttata tttttcatta tatctaaaat
    12661 ctgagcttga atagtaacat ctaaagctgt tgttggttca tcagctataa gtaattttgg
    12721 attacatgat aaagccatag ctatcattat tcgttgtctc ataccacctg ataattcatg
    12781 tggatatgaa ttatatattt cttcagctct tggtatttct accagtttta tcatatcgat
    12841 tacctttttc ttagcttctt cctttgataa atcttgatgt atcataatac cttcaatgat
    12901 ttgacttcca atagtaaacg ctgggtttaa agatgtcatt ggttcttgaa aaatcattga
    12961 tatctcattc cctcttattc ccataagttc atcttcattc atctttaaaa tatctttgcc
    13021 tcgaaagatt atttctccac cttctatttt tgatgttgga agtaacttca tcaaagacat
    13081 ggctgttaca gacttaccac atcctgattc tccaactatt ccaatggttt caccttcatt
    13141 tatattaaaa cttacttcat ttacactctt tacaatccct ttatctgtat gaaaataaac
    13201 ttttaaatct ttaacctcaa ttagtgcttt actcattatt ttctcctctc agcaaaatta
    13261 cttaagataa catacacctt ttgattgtat tttataaaaa tttaaattac aacattttct
    13321 tacattttat tatttttttc tattaattat aataatataa tagttaattt ctatatatat
    13381 tgatttaata atgattacta ttatacaata aaaaatatgt gttaaatatt attttatgga
    13441 ggttttataa gaatatgaaa tttaaaaaat tggcatcatt aatcttagtt tcatcattaa
    13501 tgcttacatt tacagcctgt gcttccaatg ataaagacaa gtctagtgga agcaaatctg
    13561 gtggaactgt tgtaatacct atggcaagtg acccagatat aatcaatgga gcttttgcaa
    13621 acgttaaaga agactctctt gcatcaaata tagtttatgc tcctctttat acttatgaaa
    13681 aaggaaattt agtaaattat ttagcagaaa aagtagattt taaagatagt aaggaattaa
    13741 ctataaaatt aaaatccaat ttaaaatggc atgatggaaa gccaataact gctgaagatg
    13801 tcttatttac atttaataca gttttagacg aaaaacaaaa ttcaccatca agacaatact
    13861 tattggtggg tgagaaacca gttaaagtag aaaaaattga tgatttaaca gttaagatta
    13921 ctttaccaac tgcaagtgaa tctttcttat atggtatatc taaaatttct ccaataccaa
    13981 aacatgtgtt tgaaggagaa tcaaatatag ctaagtcaga aaaaaataat aatccagttg
    14041 gttctggtgc attcaagttt aaggaatgga aaaaaggtga aagcatagta tttgaaaaga
    14101 atgcagatta ttttggtgga gaacctaagg cagattctat agcgctaaaa ataattccta
    14161 atgaagcatc tcaagaagca gctttaaata atggcgaaat ttcacttatg aagacatctg
    14221 ctgaaggata tgaaaaggca aaatcaaact caaacttaca aacttatact tatagtgaag
    14281 aaagattaaa ctacattgta tttaaccaaa atatatcaaa catggctaac aaagaagtaa
    14341 gacaagcctt atcatatgcc ttaaatagaa atgaaatgat agaatcagca tatggaaaag
    14401 aaggttctgt acctgctaaa tctatattag ttccagaagc agacttctat acagaagaag
    14461 gtgttgaagg atatgaccaa gatacaaata aggctaaaga cttattggat aaaagtggtg
    14521 ttaaaattga taaattaaaa ataggttata acacaggtag atttgggcat aaaaactatg
    14581 ccttagtagc tcaacaagag ttgaaaaaaa ttggtataga agctgaaata gttccttatg
    14641 aaagtaaggc attcttcaat atactattta gtaatagcac ggaatgtgat atgtatgtaa
    14701 atggttatgc ttggggatta gaaccaaacc catatagagg tatgtttgaa actggtcaat
    14761 attgcaacca aactaaatat tctaatgctg aaatagatgc tttatgggaa aaaggattta
    14821 ctgagttaaa caaggaaaaa cgtgaagaaa tatataagca aatacaacag gatatatcta
    14881 aggatgcgcc tatttacact atagattatg aacaaaattt aatggcagct caaaaaaatc
    14941 taaaaggaat aaaagatgct aaaccatctc ctgcaatatt atttgaagat tggtctaaac
    15001 tgtatgtaga ataattgatg taaaatttta tattattaat tagaaaagga gatgtctaaa
    15061 aataatattg ttttattatt ttaaaacgag acatctcttt taattaggag aatttactta
    15121 tgttaaaatt aatattgaat agagtaaaag ttttaatacc tatgttaatc ttaatatcta
    15181 tattatcatt tggactttta gaattagctc ctggtgaccc agcagatgca tatataaatc
    15241 ctttaatgtc ttcacaggat attgaaaata taagggttaa tatggggctt gataaaccag
    15301 tatatataag atatttaaac tggttgaaaa acactttaag tggaaactta ggtatttctt
    15361 atataaacca tatgcccgtt acagaacaaa ttatggaaaa aatgggaaat acatttattc
    15421 ttatgggtac ttctttgata ttttcaatat tggtagctat acctcttgga atacttttag
    15481 ctgtaaataa aaattcaata actagtaaag tatcatctgt ttttaattat ataggtgttt
    15541 ctataccttc tttttggata ggtatgatac tcatttcaat tttttcagtt aaacttaaca
    15601 ttttcccatc aggaggtatg catactattg gaaatgatag tattggagat ttagtaaaac
    15661 atttagtttt gcctgtaata actttaggtc tatataatac tgctattttt acaaattatg
    15721 tagaagctag tgtaaatgaa caattaaaaa aacaatatgt tattacagca agagcaaaag
    15781 gattatcaga aaaagtcata ctatttaaac atgtcttgaa aaattcactg acatcattgg
    15841 ttacaatcct aggcatgagt atacaaaaat tagtcactgg tgcatttgtt acagaagtag
    15901 ttttctcttg gccaggtatg ggaagattaa tgattgattc aattttttca agagattata
    15961 ctgtaattat ggcaataact atgttatcag cactattttt aattttaggt aatttagttg
    16021 ctgatatact atacttatta attgacccta aaataaaatc tagcaaagga ggattctagt
    16081 tgtgtttaca agtatcttaa aaaaattaaa aaaagataaa tcctttgctt ttgcatgtat
    16141 tactcttagc ataataatca tattaattat agcttctttt atattaaata tagactctaa
    16201 tgcaattgat actacttcta aacttcaaga acctagctta aagcatatct ttggtacaga
    16261 cgaacttggt agagattact tctctagagc tctttatggt ggaaggattt cacttagtgt
    16321 tggtatttta tcaatgctta tttctgtagt atttggtact gctgtaggtg ttattagtgg
    16381 atatattgga ggtaagctag accaattctt gatgagatta gtggatatgc ttatgagtat
    16441 accaagtttc ttagttttga ttgtattaaa tacttatcta acacctaaag tttccactat
    16501 tatactaatt attggattct taagttggat ggaagtttca agaattgtac gtggcgaaac
    16561 attaaaaata aaggaaaatg aatactgtct agctgctaaa gctactggta tcaaaactag
    16621 aaatatacta ttaagacata tagttttaaa cttaaaagag actattgtag ttgctggttc
    16681 gttaaatatt gctaatgcta tcttaactga atctgctctt agttttttag gtcttggaat
    16741 ccaattgcct cttgcatcat ggggtaatat gcttcaagat gctcaaagac atatttttga
    16801 caagttatat ttagctgtat ttccaggatt acttatattt ttaacagtca tgagtttgac
    16861 tataataagc aaaaaatttt ctagtaatac ttaacaaatt ttatatagaa aataaaaaca
    16921 gtgattatct aaagatatac actgttttta tttttcgttt aaaaaaatac atcttttgta
    16981 tattataact ttaaatacta ctctaccatg ttatgtaaag cctattattg ttctcaaaca
    17041 tatagatgaa aaactattag tttattataa taaaaattga tttatttgtt attttaataa
    17101 catttttaag agttcttttt actaaagaaa tctataataa atttaaatac atgttggtct
    17161 acatggctat aacgttcttt tttaggtcta cataatataa ctttccatgt aataggatga
    17221 ttaaaaggct tttctataat tttgctattt tcaaaaaaat catgaactgg tgatggtaaa
    17281 attgttataa aatcagaatt tgtggttgat agcaacaaaa aatcccatga accagacatt
    17341 atatatcttt ttaacttaac attttcatca gtaaattttt gcattagctt atgatgtatc
    17401 ataaaagtag gattaaatat tgcaagtaac tgctcattca aatcgttcca atctatctta
    17461 tccttcttag caagtggatg atttatattt aaaaatgctg taagttcatc ttcttgaagc
    17521 acatgttcag ttactacact tttatcaatt ttatgtggat gtaacaatac tgcataatct
    17581 aattcctgta agataagcat tctatttaag tcatatgcac ccttctctac tatatcaaat
    17641 tctatatctg gattatttgc aactaactgt gcaactacat ctgaaaatgc tattccaagt
    17701 ataagtggag gtattcctat tctaactttt cctttaaatt gaactgaatc ttctcttaaa
    17761 tcttccatca tatttttgta ttcatttatg atatgttctg catttacata aaatctttct
    17821 cctgctggtg ttaacccatt taacctgcct ttataccttt caaataaata tacatcttca
    17881 ctttcttcaa aattttttat aatttgactt aatgctggtt gagatatgtg aagtgtctta
    17941 gatgctattg ataaattaaa accagatttc actatttcaa caaaatattt caactgtttt
    18001 atatccattt taaaccccct tccccaaaag tataatttag attatatttt ttcttctaat
    18061 gatattttat aggaaaatca acataatttc attaatattt taacaaaata tttttctaaa
    18121 tctactataa atttttatcc tataaatttt tcttatacac tatataaaaa attagactta
    18181 gacaaaacgc ttttttcgcc gtaatctata attagaaaca ggaaaaccta aagcattagg
    18241 aataaaagta attgaaatac ttaaaaacaa gcttgtatta aaaaagtact ttattatagt
    18301 gttccctaat ttctttattc agaaaatttt taattatcat tcaagaaagg taactattag
    18361 atatgaaaga tgtagtaatc gtatcagcag tcagaacacc tataggaagt tttggaggtg
    18421 tatttaaaaa tacttcagct gttcaacttg gaactatcgc tgttaaagaa gcgatatcac
    18481 gtgtaagact taatttatct gaaatagatg aagtaataat tggaaatgtt cttcaaacag
    18541 ggcttggaca gaatgttgct agacaaattg ctataaatgc aggtatacca aattctgttc
    18601 caagctacac agtaaataaa ttatgtggtt cagggcttaa aagtgtccag ctagctgccc
    18661 aatcaatcac atctggtgaa aatgatgtgg taattgcagg tggtactgaa aatatgagcc
    18721 aagctcctta tatcgttcca actgctcgct ttggtagtaa aatgggtgac ataaccatgg
    18781 tagatagtat gctaacagat ggccttattg atgctttcaa tcagtatcat atgggaataa
    18841 ctgcagaaaa tattgcaact aaatttgaat ttactagaga aatgcaagat aaattggcat
    18901 tagaaagtca aaataaggct gagaatgcta taaaaaacaa ccgctttaaa gaagaaatcg
    18961 ttccagtaga tgttttaata cgtcgaggaa aaatagaaac tattgataaa gatgaatatc
    19021 ctaaattagg tatgacattt gaaggtttat ctaaacttaa acctgcattc aaaaaagatg
    19081 gtactgtaac agctggaaat gcctcaggta ttaatgatgg tgcggctatg ttaatactaa
    19141 tgagccaaca aaaagcagac gaactaggta ttagaccact tgctaaaata aaatcctatg
    19201 cttcagctgg tgttgaacct gaagtcatgg gtactggtcc aatccctgca actagaaaag
    19261 cactgaaaaa agctgggctt agcatcaatg atatagactt aatagaagca aacgaagcgt
    19321 ttgctgccca agcattagct gttaaaaatg aacttcaaat agattcatca aagttaaatg
    19381 ttaatggtgg tgcaatagca ttaggacatc caataggagc aagtggagct agaatacttg
    19441 ttaccttaat ttatgaaatg caaaaacgta aagttgaaac tggacttgca acactttgta
    19501 ttggtggtgg tcaaggaata tcaatggtgg tttcacgata ggaggaaata tgaataaaat
    19561 agttagcatt gatgaagctc tatctcatgt aaaagatgga gatacaataa tggttggtgg
    19621 attcatggcc aatggttcac cagaaaattt aattgattat ttatgtagta aaaatataaa
    19681 agatttgaca ctaatttgca atgatactgg ctttattgac aaaggagttg gcaaaatggt
    19741 tgtaaataag caatttaaaa agataattgc ttctcatgta ggattaaata aagaaactgg
    19801 aagacaaatg aacaacaatg aaacagatgt tgagttagtt cctcagggaa ctcttgctga
    19861 acaaatacgt gcagctggtt atggtctagg aggtatactc acaccgactg gtattggtac
    19921 tcttgttgag gagggaaagc aaaaaattgt tgtcgatagc aaagaatacc ttcttgaaaa
    19981 accaatttat gcagatgttg ctcttctatt tgcatcaaag gtcgataaag caggtaatct
    20041 agtttataaa ggctctatga acaattttaa taatttaatg gcatctgctg ccaaaattac
    20101 aatagttgaa gcagatgaaa tcgttgaaat tggtgatatt gacccaaatg aagttaatac
    20161 accaggtatt tttgtgaatt acatcgttga aggaggtaat ttataatgga taaattagaa
    20221 atgcaagaat atattgcaaa tagagtatct aaagaattaa aagacggagc cgttgtaaac
    20281 cttggaatag gtctaccaac caaagtagca aattatattc cggataatgt aaacgttatc
    20341 ttacaatctg aaaatggctt tttaggttta ggttcagccg aagatggaaa ttcaagtgat
    20401 gaaactattg taaatgcagg tggtcaacca gttactatac ttcctggagg atgtttcttt
    20461 gatagtgcta cttcttttgg aataatcaga ggaggtcatg ttgatataac tgttcttggc
    20521 gcacttcagg ttgacaaata tggcaatata gcaaactata tgattccagg taagatggtt
    20581 ccaggtatgg gtggagctat ggacctagta actggagcta aaaaagttat tgtagcaatg
    20641 gaacatactt caaaaggttc tgcaaaaata ttaaataatt gtactcttcc acttactgca
    20701 acaaatgctg tagatttgat agttactgaa atgggagtta tggaagtaac atcagatgga
    20761 attttactta aagaaataaa tccagctttc acattagatg atgttatttc tgctactgag
    20821 gctcctttaa tattgtcaga ttcattaaat ggaaaaatat ctgttgttta aaataattaa
    20881 aactagaaaa taaaaatctc ttagacgtat tacatataaa tttgtattgc ttaaaatatt
    20941 aatataaaaa taaaataaaa ttggaggaaa tgaaaatggt taaagataaa gttgtattcg
    21001 taacaggagc tgcaagtgga ataggtaaac aaatagctga atcgttctta aaaaatggtg
    21061 ctaaagtaat gttctctgat ataaatcaag aagctttaaa taataccata acagagttac
    21121 aaaaaaaagg ttatgattgc atgagtgtaa agtgtgatgt aactaaggaa gaagaaatta
    21181 accacgctat agacaaaact gttgaaaagt atggcagatt agatatatta ataaacaatg
    21241 ctggacttca acatgtttct atgatagaag atttcccaac tgataaattt gaatttatga
    21301 taaaaataat gctgacagca ccttttatag ctactaaaaa agcatttcct ataatgaaaa
    21361 aacaaggctt tggtcgtata ataaatatgg cctcaatcaa tggtgtaata ggatttgctg
    21421 gaaaggctgc ttataactca gcaaaacatg gacttattgg actaacaaaa gttgctgctc
    21481 ttgaagctgc cgattctggt ataacagtta atgctttatg ccctggatat gtagatactc
    21541 cacttgtcag aggtcagttc aatgaccttt caaaaactcg taatatacca ttggaaaatg
    21601 tcttagagga agttttatac ccacttgtac cacaaaaacg cctattagat gttcaagaaa
    21661 ttgccgacta tgctatgttc ttagcaagtg ataatgctaa gggtattaca ggacaagctt
    21721 gcttactaga tggaggatat actgcacaat agttattata tttagggctt gaagaaagta
    21781 ttcttcaggt ccttctacta aggagggata tttatgagta ttatcgcgcc aattggtata
    21841 attattggta tcatagctat tatatatttt tcagttaaag aaattcatat aactatagct
    21901 gctccaatag caactttaat agttgttatt ttaaataaaa tggatgttgt aacgtcaatg
    21961 ctaggtgctg acaaaaacaa ttatatgggt gcacttggaa actatataat gagttatttt
    22021 gcaatattct tattaggctc aatacttgct aaatttatgg aagaaagtgg agctactgta
    22081 tcaatagctg attttatttt aaataaattt ggctctaatc atccttatag agttttagtt
    22141 gctatattta tagtaagttt tatattaacg tatggtggta taagcttatt tgtagttatg
    22201 tttgcggtca ttccacttgc aagaactcta tttaaaaaac ttgatatttc ttggaacctt
    22261 attcagatac cactttggct tggtattgca acaattacta tgactataat tcctggaaca
    22321 ccagccattc aaaatgttat acctattcaa tatctaaata cgtctcttac tgcagctact
    22381 ataccaagta ttgtaggtag tataggttgt gctacgtttg gacttgtcta tatgaaatat
    22441 gctctaaaca aaagtattag aaaaggtgaa acttatgcaa cttatacttc agaattagaa
    22501 gaagaatctt tagatagaga attaccagga ttttttgcaa gtatttttcc tctattagca
    22561 ctcgtaatta tagctataac tggaagtact tttggttcag aatttatgaa aaaaaatgta
    22621 atttacatag ctttaatagt tgcaattctt ttatctgctt ttttattcca taaatttcta
    22681 tcatctaaga tacagacatt gagcataggt gctagtggtt ctataggtcc tatatttgct
    22741 acatcttctg cagttgcatt tggagcagta ataatggctg cacctggttt tgatgtattt
    22801 agcaaactta taatgtcaat accaggtagt ccacttataa gtcttactgt tttgacttca
    22861 acaatgtcag caataacagg gtcctcttct ggtgcattag gaatagtaat gccaaacttt
    22921 gcagattatt atttaagtac tggtctaaat ccagaattaa ttcaccgtgt tgctacaata
    22981 gcttcaaata tactaactat agttccacaa agtggtgttt ttcttacatt cttaagctta
    23041 tctaagttaa cacataaaac tggatttaaa gaaactttca ttgtagttgc agttggttca
    23101 ctaatagcag aaattattgt aattacatta ggattactaa tgtattaata taaaacaaat
    23161 taaaattcat aatatgaaag gtggtgtctc aaagtgaact ttttacttaa tgagacactc
    23221 tttttatatg aaataacata tgcctatcat ttaaacaata aaaaagaacc ttatctctat
    23281 tttgagacag acccttttta tttcttaaag atttaaataa ttataatttg ttgctaatac
    23341 gaaagcatct actgaataaa tttaaccaaa taaaaaaaaa tttatcataa ctataaattt
    23401 acgttatgga attattataa aaatattatt ttctctaact aagtaattcc atactataca
    23461 ggcttttaat acaaattgtc aaacacactg aaatatacct atttattcca tatatatctt
    23521 ttttacatat ctttacagta aaaaaagtct taatcttatg ttagtataga ttcacgggta
    23581 accccctaca ctaaaggtta caaatcagta acatttttag aatttttaga ttaagaggtg
    23641 tttaatgatg agaagaaata taccaacaaa attaaaaact atattagctt taggagtgtt
    23701 aactttagca acagcttcaa gtgctgttac tgcaagtgct tcatcacttg aaaataatgt
    23761 aaatataagt aacaaaagtt tagtatatag taatactaaa tacaagtttt ctgcaaaacc
    23821 aaattgttct gtaaaaccag aacttaagcc tgataaagat aactcttgta aggataagaa
    23881 tcataatgac aagaattgca acaacactaa taaacctgag gacaacaata attcagactc
    23941 tactaataag cctgataaca ataaacctga gtctaataaa cctgaagaca acaataattc
    24001 aggctctact aataaacctg ataacaataa acctgagtct aataaacctg aagacaacaa
    24061 taattcaggc tctactgata aacctgataa caataaacct gagtctaata aacctgaaga
    24121 caacaataat tcagactcta ctaacaaacc tgatgataat aacaactcag gctctacaag
    24181 tgaaagtttt tcagcttacc aaaaagaagt tgttgactta gtaaatatag aaagagctaa
    24241 agctggatta aatcctctta ctcttgattc ttctatatca aatgttgcta caaaaaaatc
    24301 tcaagatatg attgataaca actatttttc acacaattct ccaacttatg gttctccatt
    24361 tgacatgttg aaaaaatttg gaataagcta taagacagca ggtgaaaata tagctatggg
    24421 tcaaaaaact cctaaagaag ttgtaaacgc atggatgaat tctgaaggtc acagaaaaaa
    24481 tataatgaat ccaaatttca gtaaaatagg tgttggagta gcacaaaaaa gtggtggttc
    24541 aatttattgg acacaaatct ttgtaggata aaatactctt tttccataca tatcaagaga
    24601 gtatctctaa gtgataaaaa acacaaatga gatactcttt tgtatctatt ttaaaattat
    24661 caaatccaaa tatacatact ataaatatta tttgttctct taattttagt gtgtaaaaca
    24721 atatataaaa agtattggca agatgaaaat ggaaaactta aaaaagatat ttaataggta
    24781 aaaacaaaat cttaatatct tcataaaaaa aggctgtctc aaaatagaga taaggccttt
    24841 ttcattgttg aaatgattaa aatatatttg atttcataca aaaagagtgc ctcatgaact
    24901 aaaaagctca ctttgagaca ctctctttta tcaattctat ataagcatta taatacttta
    24961 atattattca gctaattttt tgtatccttc atatctttct ttagcatcag cttcagcttt
    25021 agcaaataat ccatcagctt cctctgggaa ttgtttagct atagcagaat atcttacttg
    25081 tgcaagtatg aaatctttga agcttgcagt tggttctttt gaatctaaag tgaatgggtt
    25141 tttaccttca gcttttactt ctggattgaa tctgtataaa tgccagtatc cgcaagcaac
    25201 agcttgtgct tcgttagcta cagttcttcc cataccttct tttaatccat ggtttataca
    25261 tggagcataa gctattatta aagatggccc atcatgtttt tcagcttcta taacggcttt
    25321 gataaattgg tttttatcag cacccatagc aacttgagct acatatacgt ttccatatgt
    25381 catagccatc attcctaaat ctttctttct agatttttta cctgaagcag caaacttagc
    25441 catagcagca gctggagtag ctttagaagc ttgacctcct gtatttgaat atacttctgt
    25501 atcaaataca agtacattta catcttcacc agatgcaaga acatggtcta atccaccgta
    25561 accgatatca tatgcccaac cgtctccacc tagtatccat tgagatctct taactagata
    25621 atcttttcta tcttttatag cttttactaa ttctttagct ttttcatctg tacaatcaca
    25681 agctggtttt tctaataatt caacaacctt agcacttgct actttagaag cttctccatc
    25741 atttctagaa tctaaccact ctgtaaatac tgctttagca tcttcacaga tatccataga
    25801 aactaactct gtcatagcat ctactatttt atttcttatt tgcttaacag ctgtgtacat
    25861 accaaatcca tattcagcat tatcctcgaa taaagaattt gcccaagatg gacctttacc
    25921 ttcatggtta caagtataag gaattgatgg tgcagaagct ccccatatag aagaacatcc
    25981 agtagcatta gctatcatca ttctatctcc atataattgt gtaactactt taatgtatgc
    26041 agtttctcca catccagcac atgctcctga aaactccatt aatggttgtc taaattggct
    26101 tcctttaaca gtgtttgcag gcataacatc accttttgga gctacctcat caggatttac
    26161 tgcaaatgcc caattatcta actctgtatc aagttgagta tctattggct tcataactaa
    26221 agctttttct ttagctggac atatgtcagc acagtttcca catcctgtac agtcaagtgg
    26281 gctaagttgc attctatatt ttaatccttc taatccttta cctaatgctt ttttagcttc
    26341 aaatccttct ggagcattag ctaattcttc ttcagtaact aatactggtc ttatacatga
    26401 atgaggacat ataaaagaac attggttaca ttgaatacag ttatctatta tccattcagg
    26461 aacatcaaca gctataccac gtttttcata agctgcagta ccacaaggga atgttccatc
    26521 ttctatacca ttgaatgtac ttactggtaa gttgtttcct tcttgtgcag tcattggtct
    26581 tagtatattt tttataaatt ctggttcttt aacttctcct ttatctgcat caacagcatt
    26641 ttcccaagaa gctggaactt caactttaac taaagcattt attcctgcat caacagcagc
    26701 atagttcatg ttaactattt tttcaccttt tttaccataa gctttaacta tagaatcttt
    26761 taagtattta acagcatctt cttctggtat tatttcagct aatttaaaga atgcagattg
    26821 cattatcata tttattctgt ttccaagacc tatttcttgt cctagtttaa cagcgttaac
    26881 tgtatagaat tttatatcat ttttagctat atattgcttc atatgagctg gtaaatgttc
    26941 ttcaagtcct gcttggtccc atatagtgtt aagcacaaat gtaccacctt ttttaagacc
    27001 ttttaataaa tcatattgat aaacatatga ttgtttatga caggcaatat aatcagcttc
    27061 atctattaaa taagtagctc ttattggagt atcaccaaat cttaagtgag acatagttat
    27121 accaccagat tttttagagt cataatcaaa gtaagcttga gcatattttt cagtatggtc
    27181 accgattatt ttgatagctt gtttattagc tccaacagta ccgtcagaac ctaatcccca
    27241 gaacttacat cttatagttc ctttagaagc tatttttatt ggctcagatg gagttaaaga
    27301 agtattagtt acatcatcaa ctatacctac tgtaaatcca tttttaggtt gttctgaaac
    27361 taagttatca aaaacagttt ttatatctga aggagtagta tcttttgaac ctaatccgta
    27421 tcttccacct attatcatag gagcattttc ttttccatag aatacatcac gtacatctaa
    27481 gtataatggt tcaccagttg aacctggctc tttagttcta tcaagtacac atattctttc
    27541 aacagtagat ggcatagcat ctaagaaatg tttcattgag aatggtctat ataaatgaac
    27601 tttaactaaa ccatattttt ctcccttagc atttaagtaa tctatagttt cttctatagt
    27661 ttctgttact gaacccatag ctattaatac atatttagca tcttctgctc catagtaatc
    27721 aaataaacta tgttttctac ctgttaaatt actcattttt tccatgtatt tttcaactat
    27781 acctactata ttttgatagt atttattaga agcttctcta gtttggaaat atatatctgg
    27841 gttttgagca gtaccacgag caactggatg atttggagat aaagcattat ctctaaatgc
    27901 ttgaactgct tcaaagttta ataaacttgc ataatcttca ttttctagta attcaacttt
    27961 ttggatttca tgtgaagttc tgaatccatc aaagaaatgt ataaatggta atctaccttc
    28021 tattgcagct aaatgtgcaa caggtgctat atcagctact tcttgaactg aaccagaagc
    28081 taataataca catccagttt gtctagcagc cataacgtct tggtgatccc caaatattga
    28141 taacgcttga gatgctaaag cacgagcact tacatggaat actcctggta acaactctcc
    28201 tgcaacttta tacatgttag gtatcattaa taataaacct tgagatgctg taaaagtaga
    28261 agttaaagct cctccttgta aagaaccgtg gaatgttcct gatgcacctg cttctgactg
    28321 catttcaaca acattaactg tttgtccaaa tatatttttt cttccttgtg atgcccactc
    28381 atcaactact tcagccatag ttgaagatgg tgtgattgga tatatagctg ctacatctgt
    28441 aaacgcatag gcaacatgag ctgcagctgt atttccatca agtgttttca taaacttagc
    28501 cataatatgt atcctccttg atagctacaa ttgtacttgt ttataacaag tctatttttt
    28561 tatatttaca tataatttac acaacaaaaa tataaacaca catatagtcg cgcaaatact
    28621 ctatcttcta atataat
//
LOCUS       DAKBNN010000024        27873 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000024 DAKBNN010000000
VERSION     DAKBNN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.024"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            201..584
                     /locus_tag="OUB02_001001"
     CDS             201..584
                     /locus_tag="OUB02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCT8884777.1"
                     /translation="MHSLFLEENYLEAIYIVSLRKKDVRNVDITKEMKIAKSSVAAAI
                     QRLVERGYITYEEDKIVRFTQSGLKIAKKIYRKHVFLAKILKHIGIDEETAEKEACKI
                     EHIISDDSFEKINNYFKKEINTLNL"
     gene            829..2190
                     /locus_tag="OUB02_001002"
     CDS             829..2190
                     /locus_tag="OUB02_001002"
                     /inference="COORDINATES: protein motif:HMM:TIGR03494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salicylate synthase"
                     /protein_id="HCT8884778.1"
                     /translation="MVKNNFENGYDKNIKKITHVVSEKMELIKLATFLATKNKIHNDF
                     IIYENNFDIYLGLGVLTKIDVKKDKFIINSSESKEVKCNELTKELKNILKGISDEDWN
                     AYGLIDFDYAKYIYMNKNNLDNKNLIEIIFPQIDINIKNTGDILIKAKNEKDLSVIEK
                     LIYEFKAVKNEYEMTDGSDDIINFGKDKYKEIVSQAVNDINNGEYEKVILSRKIPLNK
                     RVDMYQSYLYGRQKNTPARSYYIKVDGREIIGFSPETVVEVDREKNVYTFPLAGTRAL
                     CKNLEERKRLKKELLTDTKEIAEHAISVKLAFEELENCCEKDSVEIIKFMDVIERGTV
                     QHLASRLKGRLKENLNEWDALTSLFPAVTATGIPKKESIEAISRMEENDRELYSGGVF
                     KLNNFGELDVALVLRSAFQNNKESWLRVGAGIVSMSNPERELEETKEKISSVLNQIIY
                     EED"
     gene            2230..3204
                     /gene="aroF"
                     /locus_tag="OUB02_001003"
     CDS             2230..3204
                     /gene="aroF"
                     /locus_tag="OUB02_001003"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCT8884779.1"
                     /translation="MHYVFKNKSDYLKCRRFLDEYKVDYLNLKISNYYLINVFDETLK
                     EWIENKELTFKSTESISLENGYLATKNYKEKSIIKVNDIEIGNGRPVFIAGPCSVEKE
                     DTLREIAIKVKESGADVLRGGAFKPRTSPYEFQGLGKEGVDILYKIGKELDMPVITEI
                     LDVRDLDYMIDKVDILQVGTRNMYNYPLIKELGKVDKPILLKRGLSASVNEFLLSAEY
                     IMLEGNEKVILCERGIRNYDTSTRNIMDIATIALLKELSHLPIISDPSHATGKRTLIS
                     PMVDSSISAGADGVIIETCNNPDAAWSDGKQSICPKELQSIINRHKKI"
     gene            3223..3963
                     /locus_tag="OUB02_001004"
     CDS             3223..3963
                     /locus_tag="OUB02_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCT8884780.1"
                     /translation="MEKWFEVINKDIKCENIKLILFPYAGGGVSIFRNWKEFFKDIKL
                     YAAQYPGRENRMSETPINEFNTLLEKIFEEFKVIISDNKPYYLFGHSFGTKIVYELTL
                     KIISKGLPQPKGIIVSAGRAPCYKEKKPIYNLEDREFIKEINRFSGTPIEIIENIEIM
                     SLFIPMLRADFIIDETYVNENITKLDIPIHGFMGTEDKELTIKEFKKWKDYTNKEFTY
                     TYIEGGHMFINTNTEKVTNEINKLIRGK"
     gene            3964..4629
                     /locus_tag="OUB02_001005"
     CDS             3964..4629
                     /locus_tag="OUB02_001005"
                     /inference="COORDINATES: protein motif:HMM:NF013786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCT8884781.1"
                     /translation="MIKTYRFTNFKRFDFNDVIKQTCDNKVVLIIANITILRDENIIE
                     TYLSSEQIREKNNYYKQMDRNNYLLSHGIINRIFSSILRVEGKDVVWNYTNYKKPYIK
                     NKMGIKFNISHTKGAIAIALSINDVGVDIEYMEKNFNYKDIVNYSFNKNEKINTVIDF
                     YKYWVAKEAYLKYKGIGLIQDLRSIEIIEITNNRGILKDKNKTKTFEIKEINDDYSVA
                     ICF"
     gene            4642..6234
                     /locus_tag="OUB02_001006"
     CDS             4642..6234
                     /locus_tag="OUB02_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCT8884782.1"
                     /translation="MNFKIREQLEKYYDENIWEHITLGEALERWSEKYKNKIAVIDED
                     IELTYKKLNEEVDHYAEGLLNNGINKGDKVLLQLPNCAEFIIISFALFKVGAIPIMGL
                     PAHRKREIKGILEKSEAKAYIAKDKYLGFSYIDMIREIQNEIDKKINVFILGEKEEYK
                     SFYNLRWGNRISDSKKNKASYKDIGLLLLSGGTTGIPKLIPRRHTDYLYVAKECGKIC
                     KMNKDTVYLVSLPIAHNFPLGCPGVIGTLIYGGRVVICPTTSPDEIIPLIEDEEVTIT
                     GLVPAMANMCMEFLKIEEYDLSSLKVLQVGGSVLDSITAERIKNVFNCKLQQIFGIAE
                     GLICCTDLEDDIDIIYKTQGKPISKYDEIKIIDDNGEEVPDGEYGELIVRGPYTIYGY
                     YNLKEVNVNCVTEDCYFKTGDKAKKLKNGNYQIVGRLKEMINRAGEKIIPSEIEEVLL
                     KHKDIIEVQVVGVPDEILGEKIGVFIIKRNEEINLDEIRDYLKKNGLAHFKMPDIVKY
                     VESWPLTSVGKIDKSRLIELVL"
     gene            6254..12481
                     /locus_tag="OUB02_001007"
     CDS             6254..12481
                     /locus_tag="OUB02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCT8884783.1"
                     /translation="MDEKILREKAIDNIKKAIKEELNISIDIRDNLIQKGLSSIQVMK
                     ISGGLRKLGFIIPFAKLMESPTIEKWEDLIENTPIKVNKKRLSKEENLYDEFPLTDIQ
                     YAYLIGREDNQVLGGIGCHAYLEIDGANIKVDKLKSSWNKLQYFNPMLRVKFNDNGTQ
                     QIMKVPFSEDISVYDFRLFKENELENRLLEIRKKLSHRKLKVKEGEVAGLNIAILPEN
                     KYRIFLDVDLLVADVMSLNILIDELSKIYNDELILESSDYTFKDYLKNRKFDEQTMKE
                     DEEFWKNKVLTFPKESPNLPLKKKAELIDKTIFSRRRKVLDKFSWKIMKEKAAKYKVT
                     PSMVLLTCYSMIIERWINQDKFVINIPLFNRETENANIKNMIADFTSLLLVECERKEN
                     ETLLEKIRTISRTFLENVSHSSYSGVKVQRDIYKEVGKNINIAPIVFACNIDYPLETE
                     KSKKVLGEITYMISQTPQVWLDFQTYVVNGELILCWDVVDELYHEGMIDDMFDAFYEL
                     LYRLIENDNWDKVYDVLTESQKEVREKDFKNILPLEYPKKTLYNDFIEMVNKYPDNIA
                     IIDTEIERKITYKEVYDMALLIASKLISNGVNKGDYVGITLHRGYKQIIGILAILFSG
                     AAYVPIGINQPKGRRNKIYKQIGIKHVLSDENVIKSLSLYEDKIMLVDIDDLENIKSI
                     KYPVEISCFDTAYVIMTSGTTGIPKGVEIAHNSVINTIIDINKKYNICSNDTIIMVSA
                     IDFDLSVYDIFGLLSVGGTVITLNDHNFKDPDVWIKLIEKYDVTLWNSVPIIFDMLIT
                     MAEGKDIYLPIKTVLLSGDWIATTLPGRFYSRSKNSIVVGMGGATEASIWSNYINIPK
                     EIPNDWISVPYGSALKNQVYRVIDDLGRVCPNYVKGELIIGGVGVAKGYIGDKKLTKN
                     KFIQIDGIKWYKTGDMGRTWNDGTIEFLGRVDNQVKIKGHRIELGEIEDSILKNPNVK
                     NVIVEVLKIGANEQLTAFIEPNLNDNGKLSESSNILKEEIINTKVKYENYRTYLEKTN
                     IFVISTILSIFSEIGLFKENNCYTFSEIIEIGCIKVEYEKLILRWLLILEKYTVINFN
                     EGKYSLNKESEILNNILQEDTREIYNLGLLKSEILNILQGKKDAIDVFFNKDFKLSPI
                     DFLRTLKNYDKNIQRIMEIVGKLFNKQKETLRILELGGRDAKFTKSILEEIELKVSQY
                     IYCDNSLFFKDEFENIESKYDIFEFKKVNLEDNLSQVFKEKQFDLVILYNSLHRFNDI
                     AKKVKEIKNIIVDEGFIIGTEINDNSLLCEINAAILERGFENFDLSRQEGNIIPNREF
                     IKNIFCDNRIEEIYISDYGKIEETGNFLFIGRKAKCLFSSEDLKILLKDRVPEYMIPH
                     EFIEIEQIPLNKNGKVDRKKLKELIVNNEYKYKTLEYEDQMEVSDETTKILIEIWKEI
                     LLKDEISLNSNYFYVGGDSLMATKLIAKVKEKLNIEISIGDVFSHPVLKELASFIDEN
                     KEINCLNTEEGLQLDFKNKYEPFNLTDVQFAYWIGRSGAYSLGSVSTHCYFELDCFEV
                     KVERLQKIINNMIREHDMLRAIILKNGKQQILNNVPPFILIINDISSFEKDEQKVVLM
                     GIREEMSHEILKTDKWPLFKFKVSILSDNKLRLHISFDNIILDGWSMFHILGELKERY
                     DNENFILKNPGVSFRDYVLTLEKMKKSSKYEEDKNYWLSRIDDFLEAPKFEFIKSEYE
                     IKNQIFKRREKIIECAKWEKLKKVARENNITPTIFLITIFSEVLRKYSLNKEFVLNIT
                     QFSREQIHPKINDIVGDFTTLTFLEVKNTKDNTLISKAKSLQKQLTEDINHRLYSAVE
                     FGRELRLKRNNEKGSLMPIVFTSGLGLDQKRKDSWIGELVYNISQTPQVWLDHQVVEE
                     AGNLKIIWDSVDEIFPEGLLDEMFSDYIELFEIVFNANTSLNINTSIFFENSKYRIKS
                     EVGTNNTHYSKIDSIDKTINENESEEDYYENTSIEVIEILQKVLNIESIKFEDDFFKL
                     GGNSLNAVQFINLINNKYGINISMLLIAENSLVSKIVKLVSDEITSKMKSGKDKEEGF
                     I"
     gene            12494..17965
                     /locus_tag="OUB02_001008"
     CDS             12494..17965
                     /locus_tag="OUB02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCT8884784.1"
                     /translation="MTVEDIILKYQQKGVHLYLKDKKLYFDAPKGIIDEKARKEISDW
                     KNEIIHYLKYNDYEVVINKEGIYTPFKLTDIQAAYMVGSNESYSYGGIGCKIYCEIKY
                     PKLDLVKLQNAWNQVIDNNDMLHAKININGTQQILDNYILPKIKFWEMEDSKERIIDE
                     HLKNIKERLSTKKYEIGEWPLYDLEVTNLKDSSILHLSLDMMIADFMSIKIIFEELES
                     YYFEGKAKVNKELSYRDIIVYYENKKMNPNIINKYNKDEEYWKNRINSLPEAPVLPIK
                     ENSNKFSIKQYNEFINKKRKFELEKMCKEYGITVSSFILGVYTEVLRYWSQQKNFCIN
                     VTISDREDIHPEVKTTIGDFTVVNILEISDEYKLSFIERVKKIQLQLWNDMEHSSYSG
                     VEVLREMSRARNKKVVIPYVYTSALGIQDNNEFEKISKYGELIYKISQTPQVLIDCQA
                     IQYKDGILVNWDVRENDFPDGLIDSAFEVFSNILKNLEIRKLINDKNIINLPNSLRKN
                     RNKINDTYKNFERRTLVKGFCDNVKSRPEAEAISFNGVSYSYSDLADYVVTVQNELIK
                     RGCKPGDIVAIHLNKGIWQIAATLGALMVGAIYLPIDINQPISRIDRICSDSKARFVL
                     TSLNNRLGQKIYSNEICKVYIENLKVSKGLEIKKVGCNPDDIAYVIYTSGSTGNPKGV
                     VITHKAAMNTINDIIQRFNITEKDRVICISNLAFDLSVFDIFGILTAGGTLIIPSDNK
                     NVSEWNYLLKKEKVTIWNTAPAQMQMITSYLESEKINGSDSLRVVMLSGDWIPLSLPN
                     EIKSLFKNSRIISLGGATEASIWSIFHEIDIDNNYKRSITYGTPLANQQFYVLDSNLN
                     ECPDWVRGTLYIGGKGLANGYLNDRDLTAQKFIFHNNLGKRLYNTGDCGRYYPNGDIE
                     FLGREDGQVKIRGNRVELNEIEAILNTFPGIANSTVVISGENDDIIGAFIQPDISSYC
                     KESNVNQRIRTKNELLFKNKEKYGIVKQFNEWIEASNNTAILDILKTFQSIGIFTDFK
                     IWHTENEIINKIGIKSSYISLLRRWLKVLENERIIEKNEEDGYKAIMYVDGNEADNEW
                     AKWTKLEEKVHYSEVMMKYFSESRKNLIPLLRGEIDPIELFFPGGKFDIALEAYKYNK
                     VNSFMNKTIIDNIKLILDDFMKIDSKNKFKILEVGAGVGGVSQELIESIKEYPVEYMF
                     TDISQSFLNFARSNFKQYPWVEFQLYDINRNYWDQNINSSSYDLILCNNVLHNASDLL
                     RVMKQIKEMVKPNGYIVILEETENNYTLLTSVEFLNGFNNVKDFRNENNQVFLDKNQW
                     GTIIEKIGGNIVSILPKINESLNGFGQTLFVIQIESKIKSLNENKIKSYLKQNLPEYM
                     IPNHIEILKELPITVNGKIDRKALMNRVKSEKEEVQNKTEKIQLKTDLEKRVAEIWKL
                     ILKRRDIYANENFYEMGGDSLVAAQLVAQLKENIVEAKEWPWDKLMVALIESPTVGGL
                     CKRLIEKQSTMSSKKENKMSGLIYLKEDPNSSITRVLIHDGTGTINPYDKLIPLMKEL
                     DGDLVAIICDNTEKYLSIKSDELIQTLGIKYAKKLIESSEKDFELIGFCIGGLIALET
                     AKVLSENGKKVTRLISIDTTPSKKMIYNELLMERAFGMIIGSNVYRAGHMVEDFELKK
                     AILSLGEKYGYGSVISNEELLVMKEEFNNVIKCYEKLALIDKKERFKKIYSNIYKYGD
                     KNIIYNEEQLPPIYEVFCHSFKAMISYNPGVYFGKTVILDCSNQKTTFLPIDDCDNEL
                     FWKNVIIGESSRVRIEGNHLTCLQESYVKNVFHLLYKEM"
     gene            17968..19086
                     /locus_tag="OUB02_001009"
     CDS             17968..19086
                     /locus_tag="OUB02_001009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884785.1"
                     /translation="MKRKVGILGYGGKVGQIVVDILKEKYLLKCGQRSISEDIYINSD
                     DTIEYCKIDICNHEMLKEFCLDCDLLINCTGPSYFTSCKIAEEIQKYDIKFVDIFGVT
                     LLNNKKALSNLTGVIGAGSFPGFSAILVSWIAERYGKSIENLSIYAGGKELITTNACV
                     DVLMSMVENFGKTDRYIFNGSVSINERKPNESKLFNIFGRKAYINEYLTDEIYNAGIK
                     YGIKELHWYNVQINPLYKTVMDRAVMDLAKIKSKDDIYQVAEKIKIDLEKSNYNSNNW
                     YKYWIEITINNGFGKYLENIEVVCSDTYKINGIIAAMCVERLLNNKYHKNIYWAFDLL
                     DSEIVIKNLVSNGAITDIKVGNESIMNKLFTEIEEGEI"
     gene            19083..20162
                     /locus_tag="OUB02_001010"
     CDS             19083..20162
                     /locus_tag="OUB02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT8884786.1"
                     /translation="MNDKVKLKILVCGVGFGQFYLKALERLKDEYELVGIFSRGSEIS
                     KRYAKKYKVPLLTDISGISKNDVDVACVVINSTLFGGKGSHIVEDFLKKGINVIQEQP
                     VHIDDYINFLKLAQLHKCKYKLNTFYPDLRIVDNLIKIVKKLNDYSELRYITAECSVH
                     VLFPLIDILGRALNGIKPWKFKKISDVKTDSPFCIVSGYINDIPICINIQNQIVSKNP
                     DNYMLLLHRIGFTTNCGNLLMTGTNGVIIWEPCIEKQTNEEGKFDLEVQNEFSKLNTF
                     EIVNKQENITFYDMMLGDWVNGLVSSVLEFKDEIIKEKEVGNNEQYLIAVIQAWHDLA
                     IEIGRPTIINEYYKSPLSLKELMKF"
     gene            20194..20790
                     /locus_tag="OUB02_001011"
     CDS             20194..20790
                     /locus_tag="OUB02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCT8884787.1"
                     /translation="MYKNKGLTIKNMVTIGVFSAIYCVFYIIGGIPFGINPVLTFYQA
                     MGSALLCGPVYMLLVAKVGKKWSVFILGIVVGILLFITGMHWAMVLGCIIMGFIADII
                     ARIGNFKSKKMNIISYMIFSLGGVGSFLAYFINPNGWIRAMVEKGTDQSYLDTMNAVA
                     KPWMIYVIIIGTLLVAAFSAWVGSKLLKKQFEKAGIVD"
     gene            20790..21500
                     /locus_tag="OUB02_001012"
     CDS             20790..21500
                     /locus_tag="OUB02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCT8884788.1"
                     /translation="MIYFSKEGLRFDPRIKILILFICVITATITPSLDYELEFVLLIG
                     VFAIVNKKWKYALKTIVIYVFIYFLSVWTIYNAPGSLRGILMAFLGLVHKVYPCAMMS
                     GIVISTTKVSEFLVAMNKSRIPQKLIIPIAVMLRYLPSIQEDWHYIKDAMKMRGISPS
                     LKGFITSPSITVNCIYVPLMMAASKVADELTIASVTRGIENPKPRTSLIQIKINKVDI
                     CALLIFVLFLIWGIYLGR"
     gene            21509..22969
                     /locus_tag="OUB02_001013"
     CDS             21509..22969
                     /locus_tag="OUB02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT8884789.1"
                     /translation="MIEFKNISFSYCGQKQGSLKEINLHIKKGECVLLCGRSGCGKTT
                     IIRLINGVIPHFYTGDLVGDILIEGEHISEFPMYKIAEYVGSVFQNPRTQFFNIDTDS
                     EIAFGLENMAYDSERIANIVEKVAIDLDIEKLRGRSIFKLSGGEKQKIAFASVYAMNP
                     DIFLLDEPSSNLDMISIKNLRKKLKKLKDKGKTILIAEHRIFYLMDLVDKIIYLEEGK
                     IAKVYCPKEFRKIPLEQRKAMGLRSINLSEENFKYRNLLNGKKDLKINNLTVFYKKRD
                     IIKNCSFQVDKGEIIALTGNNGEGKTTFSRVLCGLHKEYIGDIFWGNKQIKEKERLKI
                     SYMVMQDVNYQLFAESVQEECLLGIKNPDKNKVNEILKRLDLYNFKNLHPNRLSGGQK
                     QRVAVAVSMICNKEILIFDEPTSGLDYESMIKVSKLLKELASMGKIIFVITHDYELIC
                     QVCTRILHLSGGKFKYDLNLDEENFKIIEKIFTNKE"
     gene            complement(23150..24139)
                     /locus_tag="OUB02_001014"
     CDS             complement(23150..24139)
                     /locus_tag="OUB02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884790.1"
                     /translation="MKKFENMLKDIGYTECSKCQKYSSAGNAFYIDNEEITGIYWFYE
                     TEYFIIDIHDFFIKKEMIVDSFPTIKPYMSFSSTYIITANGEWFNPYQNMTSNTVFIM
                     NVDNSDIRFLLHGNHPYFSVGVNFKKNMIEKYLDTTNDDISNIFLDTRDLITKHINKL
                     ANSILNCKMTSTSASLFFEAKAKEWLSITLNAYEQKKYVKPISEKDEKAIENVANYID
                     DHLTFDIPQELLEKIAMMSGTKLKNTFKHKYQMSITEYTQRKRINIAENLILTTNLDI
                     KDIAKSVGYNSHSRFTTLFKRYKNIYPKDLKGSNDQYIQSICCKNKNNNKIKP"
     gene            24345..26102
                     /locus_tag="OUB02_001015"
     CDS             24345..26102
                     /locus_tag="OUB02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884791.1"
                     /translation="MFKVYRKLLKYVPEKKYLAYISILLTTVSTVVTVGSYYYLSEFL
                     KGLIVNNNIYKSKEYALIITGMLAIGAILYFIAVFICHIIGFRLETNLRKRGIDGLTK
                     ASFRFFDLNSSGKIRKIIDDNASQTHQIVAHLIPDNAGAVLTPIFILILGFFIGIKVG
                     VVLVSLTIVSGILLKLMMGEQEFMKYYQKALENLSSETVEYIRGIQVIKIFGTDVSSF
                     KTLYKAIKDYSKYAHDYSLSCKKPYVRYQWFFCGVVLITIPIVALTMDISKNPQILAV
                     ELIMILFLSGIIFNSFMKVMYVSTYAFQGISAVDKLEELFNEMQKDKLRFGNKDNFNN
                     FDIEFENVSFGYTKDMIIDNLSFKLKQNKSYALVGASGSGKSTIAKLISGFYKVNKGV
                     IKIGGEPIEKYREECLIKNIAFVFQNVKLFKTTIYENVKLANEKATREEVINALYLAG
                     CGSILDKFKDRENTVIGSKGVYLSGGEKQRIAIARAILKDANIVILDEASASVDPENE
                     HELQIAFSNLMKGKTVIMVAHRLTSIRNVDEILVIEDGKIIERGNDLELMSIDSKYRE
                     FQETYGKANNWRIGYGEFC"
     gene            26086..27807
                     /locus_tag="OUB02_001016"
     CDS             26086..27807
                     /locus_tag="OUB02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884792.1"
                     /translation="MENFVKNKFALTEQGAKDLVKASFVSFLIYAINMVPSILLMIFI
                     DELVLGNIKSNEFYIGFSIIVLVIMYLLLSIEYDFLYNATYKESANLRIDIGQRLSRL
                     PLSYFSKHDLSDLSQTIMSDVEAIEHAMSHAIPKVLGFYIFFPLLSLMLILGNLKLAF
                     AVILPVILSFLLMYISKKTQLNEIKKHYLKLRENSESFQETIEMGQDIKSFGLAEKFK
                     RNLYKKMEDGEKLQLKSNCVQVIPVASAGIVMYISIAVVILIGTDMYISGEINILYLI
                     GYLLAAVKIKELVDGITENVSELFYLDSRIKRIKEIRGNEIQEGKEVNIKNFNIKLEN
                     VTFGYNNNNKILKGISFIAKQGEVTALVGMSGCGKTSILRLISRLYDYDNGKISIGNI
                     DIKDISTKALFQKISIVFQDVTLFNTSIMENIRIGKKGATDEEVREAACLANCEEFIK
                     KLPHKYNTLIGENGASLSGGERQRLSIARAFLKDAPIVILDEIAASLDVENENKIQES
                     LNRLMKDRTVIIISHRLKSIENVDKIVVINDGKVECFGKHCELLEKSNLYKKLVEEER
                     LVEEFKY"
BASE COUNT        11345 a         2418 c         4905 g         9205 t
ORIGIN      
        1 ttataaaaat tattttataa aattagaaat aaaaaaacat agaattatat agtaaattat
       61 aataaaatgg agagaatggt gtttttatat ttaaatatat atttggaaaa ttaggttaaa
      121 ttactataaa ttgctgatga tatacattga aataaagaca tatagaaatt agatatatta
      181 tatttgagag agggaataaa atgcacagtt tatttttaga agaaaattat ttagaagcta
      241 tatatatagt atcattaaga aaaaaagatg ttagaaatgt ggatattaca aaagaaatga
      301 aaattgctaa gtcgtcagtt gcagcagcta ttcaaaggtt agttgaaaga ggatatataa
      361 cttatgaaga agataaaata gtaagattta cacagtcagg tttgaaaata gcaaaaaaaa
      421 tttacagaaa acatgtattt cttgcaaaaa tattgaaaca tattggaata gatgaagaaa
      481 ctgctgaaaa agaagcatgt aaaatagagc atattatatc tgatgatagt tttgaaaaaa
      541 taaataatta ttttaaaaaa gaaattaata ccttaaacct atagaaagac aaaatttagt
      601 ccttctatag gttttttgat tataaagaaa gtatttaaaa ttttaaggat aacttattta
      661 ttagcaatat taatgtgttt atgtattata aagaaaagta taaattaata gattattatt
      721 tttaaaataa atgatattga aaatctttat caaaaatgat acactaaaaa atataaaagt
      781 aaggactata gaacttactt ttatataaat tggagaggga gatgttatat ggtaaaaaat
      841 aattttgaga atggatatga taaaaatata aaaaaaataa ctcacgtagt ttcagaaaaa
      901 atggaattga taaaattagc tacttttcta gcaactaaaa ataaaataca taatgatttt
      961 attatatatg aaaataattt tgatatttat cttggtttag gtgttttaac taaaatagat
     1021 gttaagaaag ataaatttat tattaattct tcagaatcaa aagaagtaaa gtgcaatgag
     1081 ttaactaaag aattaaaaaa tattttaaag ggaatatccg atgaagattg gaatgcatat
     1141 ggattaattg attttgatta tgcaaaatac atatatatga ataaaaataa cttagataat
     1201 aaaaatttaa ttgaaattat ttttccgcaa attgatataa atataaaaaa tacaggtgat
     1261 attttaataa aagctaagaa tgaaaaagat ttatcagtta ttgagaaatt aatatatgaa
     1321 tttaaggctg ttaaaaatga atatgagatg acagatggtt ctgatgatat tattaatttt
     1381 ggtaaagata aatataaaga aatcgttagt caagctgtaa atgacattaa caatggtgaa
     1441 tatgaaaaag taattctgtc cagaaaaata ccattaaata aaagagtaga catgtaccaa
     1501 agttatttgt acggacgtca aaaaaatact cctgcaagat cttattatat aaaagttgat
     1561 ggaagagaaa ttataggttt tagtcctgaa actgtagtag aagtagatag agagaaaaat
     1621 gtatatactt ttccacttgc aggaacaagg gcattatgta aaaacttaga agagagaaaa
     1681 aggttaaaga aggaattatt aacagataca aaagaaattg cagaacatgc tatttctgtt
     1741 aaattagctt ttgaagaact tgaaaattgt tgtgaaaaag atagtgttga gattataaag
     1801 tttatggatg ttatagaaag aggaactgta caacatctag catcaagatt aaaaggaaga
     1861 cttaaagaaa atttaaatga atgggatgct ttaacttctt tatttccagc tgttacagca
     1921 actggaatac ctaaaaaaga aagtatagaa gcaatttcca gaatggaaga aaatgataga
     1981 gaattatata gtgggggagt atttaaactt aacaactttg gagaacttga tgtcgcatta
     2041 gtattaagaa gtgcttttca gaataataag gaaagttggc ttagagttgg agctggtatt
     2101 gtaagtatgt caaatccaga aagagaatta gaagaaacga aagaaaagat aagtagtgtt
     2161 ttaaatcaga ttatttatga agaagattga tgttaattta tttaataaaa aagtaaaggt
     2221 ggtgaatgga tgcactatgt ttttaaaaat aaaagtgatt atttaaaatg taggcgtttt
     2281 ttagatgaat ataaagtaga ttatttaaat ttaaaaattt caaattacta tttaattaac
     2341 gtatttgatg aaaccttaaa agagtggata gagaataagg aattaacatt taaaagtact
     2401 gaaagtatat ctttggaaaa cggttattta gctacgaaaa attataaaga aaaaagtata
     2461 ataaaagtta atgatattga gataggtaat ggtagacctg tctttatagc agggccatgt
     2521 tctgttgaga aagaagatac tttacgagaa atagcaatta aggtaaagga aagtggagca
     2581 gatgtattaa gaggaggtgc atttaaacct agaacgtcac cttatgaatt tcaaggactc
     2641 ggcaaagaag gtgtagacat tctttataaa ataggaaagg aactagatat gcctgtaata
     2701 acagagattt tagatgttag agatttggat tatatgatag ataaggttga tattttacag
     2761 gtaggaacaa ggaatatgta taattatcca ttaattaaag agcttgggaa agtagacaaa
     2821 cctatattat taaaaagagg tcttagtgcg agtgttaatg agttcttatt aagcgcagaa
     2881 tatattatgc ttgaaggaaa tgaaaaggtt attctttgtg aaagagggat aagaaattat
     2941 gatacatcta ctcgaaatat aatggatatt gcaacaatag ctcttttgaa agaattaagt
     3001 catttaccaa taatttcaga tccaagtcat gcaacaggaa aaagaacatt aatatctcct
     3061 atggttgatt cttcaatttc agctggtgca gatggtgtta ttatagaaac ttgtaataat
     3121 cctgatgctg catggagtga tgggaaacaa agtatttgtc caaaagaact tcaatctata
     3181 ataaatagac ataaaaaaat ttaaataagt gaggaaataa ttatggagaa atggtttgaa
     3241 gtaataaata aagatattaa atgtgaaaat ataaaattga ttttatttcc atatgcagga
     3301 ggtggggtat caatttttag aaattggaaa gagttcttta aagacataaa actttatgca
     3361 gcacagtatc caggaagaga aaatagaatg tcagaaactc ctattaatga atttaatact
     3421 cttttagaaa aaatatttga agaatttaaa gtaataatat cggataataa accttattat
     3481 ctatttggtc atagttttgg aactaaaatt gtttatgaac ttacattaaa aattatttca
     3541 aagggtttac ctcagcctaa aggaataata gtttcagcgg gaagagcacc ttgctataaa
     3601 gaaaaaaaac caatttataa tttagaggat agagaattta taaaagaaat taatagattt
     3661 tcaggaacac caattgaaat aatagaaaat attgaaatta tgagtttgtt tattccaatg
     3721 cttagagcgg atttcataat cgatgaaaca tatgtaaatg agaatataac aaaattggat
     3781 attccaatac atggatttat gggtactgaa gataaagagc ttactataaa ggaatttaag
     3841 aaatggaaag attacaccaa taaggagttt acttatacat atattgaagg tggtcatatg
     3901 tttattaata caaatacaga aaaggtaact aatgaaatta ataaattaat tagaggaaaa
     3961 taaatgataa aaacatatag atttactaat tttaaaagat ttgattttaa tgatgttatt
     4021 aagcagacat gtgataataa agtagttctt ataattgcta acataacaat attgagagat
     4081 gaaaatatta tagaaactta tttaagtagt gagcaaattc gggaaaagaa taattattat
     4141 aaacagatgg atcgcaataa ctatctttta tcacatggaa ttataaatag aatattttca
     4201 agtattttaa gagttgaagg gaaggatgta gtatggaatt atacgaatta taaaaaacca
     4261 tatattaaaa ataagatggg aataaagttt aatatatctc atactaaagg agcgatagca
     4321 attgcattat cgataaatga tgtaggggta gatatagaat atatggaaaa aaactttaac
     4381 tataaagata ttgtaaatta ttcatttaac aaaaatgaaa aaataaatac ggttatagat
     4441 ttttataagt attgggtagc aaaagaggct tatctaaaat acaaaggaat aggtttgatt
     4501 caggatttaa gatctataga aattattgag attactaata atagggggat attaaaagac
     4561 aagaataaga ctaaaacttt tgaaattaaa gaaattaatg atgattattc ggtagctata
     4621 tgcttttaat ggaggaaaat tatgaatttt aaaataagag aacagttaga aaagtattat
     4681 gatgaaaata tatgggaaca tattacattg ggggaagctt tagagaggtg gagtgaaaaa
     4741 tataagaata aaattgcagt tatagatgaa gatattgagt taacttacaa aaaattaaat
     4801 gaagaagtag atcattatgc agaagggttg ctgaataatg gaattaataa aggggacaaa
     4861 gtcttgttac aattacctaa ttgtgcagaa tttattatta tttcatttgc tctttttaaa
     4921 gttggagcta ttccaattat gggacttcca gctcatagga aaagagaaat aaaaggaata
     4981 ttagaaaagt cagaagctaa ggcttatatt gctaaagata aatatttggg gttttcttat
     5041 atagatatga ttagagaaat acaaaacgaa attgataaaa aaataaacgt attcatacta
     5101 ggagaaaaag aagagtataa gagtttttat aatttaaggt ggggaaatag aatttctgac
     5161 agtaaaaaga ataaagcgag ttacaaagat attgggttat tattattatc aggtggaaca
     5221 actggaattc ctaaattaat accaagaaga catacagatt atttatatgt agcaaaagag
     5281 tgtgggaaaa tatgtaaaat gaataaagat acagtttatc tagtatcttt acctatagct
     5341 cataactttc ctttaggatg tcctggagtt ataggcacat taatatatgg tggaagagtt
     5401 gttatttgtc caactacaag tcctgatgaa attatacctc ttattgaaga tgaagaagtt
     5461 acaattacag ggctagttcc tgctatggca aatatgtgta tggaattttt aaaaatagag
     5521 gaatatgacc tatcctcact aaaagtttta caggtaggtg gatctgtttt agattcaatt
     5581 acagctgaaa gaataaaaaa tgtttttaat tgtaaattac aacaaatttt tgggatagca
     5641 gaaggactaa tatgttgtac tgacctagag gatgatatag atataatata taaaacacaa
     5701 ggtaaaccaa ttagtaaata tgatgaaatt aaaattattg atgataatgg agaagaagtt
     5761 ccagatggag aatatggaga gttaattgtt agaggtccat atactattta tggttattat
     5821 aatttgaaag aagtgaatgt aaattgtgtt actgaagact gttactttaa aacaggggat
     5881 aaagcaaaga agttgaaaaa tggtaattat cagattgttg gaagattaaa agagatgatt
     5941 aatagagctg gagaaaaaat aataccttct gaaatagaag aggttttact aaaacataaa
     6001 gatattatag aagtacaagt agtaggagtt cctgatgaaa tattaggaga gaaaatagga
     6061 gtatttataa taaaaagaaa tgaagaaatt aatttagatg aaataagaga ttaccttaag
     6121 aaaaatggct tagcacattt taaaatgcct gatatagtaa aatatgttga aagttggccg
     6181 cttacaagtg ttgggaaaat agataagagt aggttaatag agttagtttt gtaaaataag
     6241 gaggtcagtt agaatggatg aaaaaatatt aagagaaaaa gctatagata atataaaaaa
     6301 ggctataaaa gaagaattaa atatttctat agacataaga gataatctta tccaaaaagg
     6361 tttaagttca attcaggtaa tgaaaatatc aggagggtta agaaaacttg gatttatcat
     6421 accttttgcg aaattaatgg aaagtcctac aatagaaaaa tgggaggatt taattgaaaa
     6481 tacaccaata aaggtaaata aaaaaaggtt atcaaaagaa gaaaatttat atgatgaatt
     6541 tccgctaact gatattcaat atgcttattt aattggtaga gaagataatc aagtacttgg
     6601 tggtattggt tgtcatgctt atttagagat agatggtgca aatataaaag tagataaact
     6661 taaaagttca tggaataaat tacaatattt taatcctatg ttaagagtta agtttaatga
     6721 taatggtacg caacaaataa tgaaagttcc attttcagaa gatataagtg tatatgattt
     6781 taggttattt aaagagaatg aattagaaaa tagactttta gaaattagaa aaaagttatc
     6841 tcatagaaag ttaaaggtta aagaaggaga agttgcagga cttaatattg caatattacc
     6901 tgaaaataag tatagaatat ttttagatgt tgacttactt gttgccgatg taatgagttt
     6961 gaatatttta attgatgaat tatctaagat atataatgat gaactaatct tagaatcatc
     7021 tgattatacc tttaaagatt atttaaagaa tagaaaattt gatgaacaaa ctatgaaaga
     7081 ggatgaggaa ttctggaaaa ataaggtttt aacatttcca aaagagtcac ctaatttgcc
     7141 attaaagaaa aaagcagaac taatagataa aacaatattt tcgagaagaa gaaaggtatt
     7201 agataaattt tcatggaaaa ttatgaaaga aaaagctgca aaatataagg ttactccttc
     7261 aatggtgctt ctaacttgct attccatgat tattgaaaga tggataaatc aagataaatt
     7321 tgtaattaat attcctttat ttaatcgaga aactgaaaat gcaaatataa aaaatatgat
     7381 agctgatttt acaagtttat tattggttga gtgtgaacga aaagaaaatg aaacattact
     7441 tgaaaaaata agaactatat ctagaacatt tttagaaaat gtttcgcata gttcttactc
     7501 aggagtaaaa gtacaaagag atatttataa ggaagtaggt aaaaatataa atattgcacc
     7561 tatagtattt gcatgtaata ttgattatcc attagaaact gaaaaatcta aaaaagtact
     7621 tggagaaatc acatatatga tttcacagac accgcaggtt tggcttgatt ttcaaactta
     7681 tgtggtaaat ggagaactta tattgtgttg ggatgtagta gatgaattat atcatgaagg
     7741 aatgattgat gatatgtttg atgctttcta tgaactatta tatcgtttaa ttgagaatga
     7801 taattgggat aaagtatatg atgtattaac tgaaagtcaa aaggaagtta gggaaaaaga
     7861 ttttaaaaat attttacctt tagaatatcc taaaaaaaca ttatacaatg actttattga
     7921 aatggtaaat aaatatcccg acaatattgc aattatagat acggaaatag aacgaaagat
     7981 aacatataaa gaagtatatg atatggcgct attaattgcc tctaaattga tatcaaatgg
     8041 agtgaataaa ggagattatg ttggtattac tttacataga ggatataaac aaattattgg
     8101 aatacttgca atcttatttt caggagctgc atatgtacct ataggaatta atcaaccgaa
     8161 aggaagaaga aataaaattt ataaacaaat aggtattaaa catgtcttaa gtgatgaaaa
     8221 tgtaataaaa agtttatcgc tttatgaaga taaaataatg ttagttgaca ttgatgattt
     8281 agaaaatata aagagtataa aataccctgt agaaataagt tgttttgata cagcatatgt
     8341 aattatgaca tcaggaacta caggaattcc caaaggagta gaaattgctc ataatagtgt
     8401 tataaatacg ataatagata taaataaaaa atataatata tgcagtaatg atactattat
     8461 tatggtttca gctattgatt ttgatttatc tgtgtatgat atatttggat tattgtcagt
     8521 aggagggaca gttataacat taaatgatca taactttaaa gatcctgatg tttggataaa
     8581 actaatagaa aaatatgatg ttaccttatg gaattctgtt cctattattt ttgatatgct
     8641 tattactatg gcagaaggaa aagatatata tcttccaatt aagacggtat tactttctgg
     8701 ggattggata gcaacaacat tgccaggaag attttatagt agaagtaaaa actctatagt
     8761 ggtagggatg ggtggagcaa cagaagcatc tatttggtca aactatatta atataccgaa
     8821 agagatacct aatgattgga tttctgttcc atatggaagt gcgttgaaaa atcaagttta
     8881 tagagtaata gatgatttag gaagggtttg tccaaattat gtaaagggag aacttataat
     8941 aggtggagta ggtgttgcta aaggatatat aggtgataaa aaattaacta aaaataaatt
     9001 catacaaatt gatggtatta agtggtataa aacaggggat atggggagaa cttggaatga
     9061 tggaacaatt gaatttttag gaagagttga caatcaagtt aagataaaag gtcacagaat
     9121 agagcttgga gagatagaag actctatttt aaaaaatcct aatgttaaga atgtaattgt
     9181 agaagtttta aaaattggtg caaatgaaca attaactgct tttattgaac ctaatttaaa
     9241 tgataatgga aaattatcag agtctagtaa tatattaaaa gaagagatta tcaatacgaa
     9301 agttaaatat gaaaattata gaacttattt ggagaaaaca aatatatttg taataagtac
     9361 tatattaagt atatttagtg agataggttt gtttaaagaa aataattgct acactttttc
     9421 agagattatt gaaataggtt gtatcaaagt agaatatgaa aaattgattt tacgttggct
     9481 attaatttta gaaaaatata cagttataaa ttttaatgaa ggtaaatact cgttaaataa
     9541 agaaagtgag atattaaata atattttaca agaagatact agagagattt ataatttagg
     9601 gttattaaaa tctgaaatat taaatatatt acaagggaaa aaagatgcta ttgatgtatt
     9661 tttcaataaa gattttaaat taagtcctat agatttttta agaactttaa aaaattatga
     9721 taaaaatatt caaagaatta tggaaatagt aggaaaatta tttaataaac aaaaagaaac
     9781 attgagaata ttagagctag gtggtagaga tgctaagttt acaaagtcaa tactagaaga
     9841 gattgaacta aaagtttctc aatatatata ttgcgataat tcactatttt ttaaagatga
     9901 gtttgaaaat attgaatcta aatatgatat ttttgaattt aaaaaggtaa atttagaaga
     9961 taatctttca caggttttta aagaaaaaca atttgactta gtgatacttt ataattcctt
    10021 acaccgtttt aatgatatag ctaaaaaggt taaggaaatt aagaatatta tagtagatga
    10081 aggatttata ataggaactg aaattaatga taactctctt ttgtgtgaaa taaatgctgc
    10141 aatacttgaa agaggatttg aaaattttga tttaagtaga caagaaggaa atatcattcc
    10201 taatcgagag tttataaaaa atattttttg tgataataga atagaagaga tttatatttc
    10261 agattatgga aaaattgaag agacaggtaa ttttctattt attggaagga aagcaaaatg
    10321 tttattttca tcagaagatt taaagatatt actaaaagat agagttcccg aatatatgat
    10381 tcctcatgaa ttcatagaaa ttgaacaaat tcctttaaat aaaaatggaa aagtagatag
    10441 aaaaaaactt aaagaattaa ttgttaacaa tgaatacaag tataaaacat tagaatatga
    10501 agatcaaatg gaagttagtg atgaaactac aaaaattttg attgaaatat ggaaagaaat
    10561 attattaaaa gacgaaatta gtttaaactc aaactatttt tatgtaggtg gagattcatt
    10621 gatggcaaca aagttgatag ctaaagtaaa agaaaaatta aatattgaaa tttctatagg
    10681 agatgtattt agtcatcctg tattaaaaga gttagctagt tttatagacg agaataagga
    10741 gattaactgt ttaaacactg aagagggttt acaattagat tttaaaaata aatatgaacc
    10801 attcaactta actgatgtac aatttgcata ttggattgga agaagtggag catattcttt
    10861 aggttcggtt tcgactcact gttattttga actagattgt tttgaagtaa aggtagaaag
    10921 actacaaaag attatcaata atatgataag ggaacatgat atgcttagag ctattatttt
    10981 aaaaaatgga aaacagcaaa tattaaataa tgttccacca tttatattaa ttattaatga
    11041 catttcatct tttgaaaaag atgagcaaaa agtggtatta atgggaatta gagaagagat
    11101 gtctcatgaa attttaaaga ctgataagtg gccattattt aaattcaagg taagtatttt
    11161 gagtgacaat aaactaagat tgcatataag ttttgataat attattttgg atggatggag
    11221 tatgtttcat attttaggtg aattaaaaga aagatatgat aatgaaaact ttattttgaa
    11281 aaatccagga gtttcattta gagattatgt tttaactctt gagaagatga aaaaatcttc
    11341 aaaatatgaa gaagataaaa actattggct aagcagaata gatgattttt tagaagctcc
    11401 taaatttgaa tttataaaat cagaatatga aataaaaaat cagattttta agagaagaga
    11461 aaaaattatt gaatgtgcta aatgggagaa attgaaaaaa gtagcaaggg agaataatat
    11521 tactcctaca atatttttga ttactatatt ttcagaagtt ttacgaaaat atagtttaaa
    11581 caaagaattt gtgttaaata tcacacaatt tagtagagaa caaattcatc ctaaaattaa
    11641 tgatattgtc ggagacttta caactttaac ttttttagag gttaagaata ctaaggataa
    11701 taccctaatt agtaaggcta aaagtttgca aaaacaatta acagaagata ttaaccatag
    11761 attatatagt gctgtagagt ttggaagaga attaagactg aaaagaaata atgaaaaagg
    11821 atctttaatg ccgatagtat ttacaagtgg attaggtttg gatcagaaga gaaaggacag
    11881 ttggatagga gagcttgttt ataatatttc tcaaactccg caagtttggc ttgatcatca
    11941 agtggttgag gaagctggga atcttaagat aatatgggat tcagttgatg aaatatttcc
    12001 agaaggttta ttagatgaga tgtttagcga ttatattgag ttatttgaaa tagtttttaa
    12061 tgctaatact agtcttaata ttaacacaag tattttcttt gaaaatagta aatatagaat
    12121 taaatctgaa gttggtacta ataatacaca ttattctaag attgattcta tagataaaac
    12181 tattaatgaa aatgagtcag aagaagatta ctatgagaat acatctatag aggttattga
    12241 aatattgcag aaagttttaa atattgaaag tataaaattt gaagatgatt tttttaagtt
    12301 gggaggaaac tctttaaatg ctgttcaatt tataaattta attaataata agtatggtat
    12361 taatatatct atgcttttaa tagcagagaa ctcattagta tcaaaaatag ttaaattagt
    12421 tagtgatgag ataacttcta aaatgaagag tgggaaagat aaagaagaag gatttatata
    12481 gggaggtaaa aatatgactg tagaggatat aattttgaag tatcagcaaa agggtgttca
    12541 tttatattta aaagataaaa aattatattt tgatgctcca aaaggaataa ttgatgaaaa
    12601 ggcaagaaaa gaaataagtg actggaaaaa cgaaataatt cattatttaa aatataatga
    12661 ttatgaagtt gtaataaata aagaaggaat atatacccct tttaaattaa cagacatcca
    12721 agcggcttat atggttggta gcaatgaatc atatagttat ggtggaattg ggtgtaaaat
    12781 ttattgtgaa ataaaatacc ccaaattaga tttagtcaag cttcaaaatg cttggaatca
    12841 agtaattgat aataatgata tgcttcatgc aaaaataaat attaatggaa cacaacaaat
    12901 acttgataat tatatattac ctaaaattaa gttttgggaa atggaggatt ctaaagaacg
    12961 aattatagat gaacacttga aaaatattaa ggaaagatta tcaactaaga aatatgaaat
    13021 tggagaatgg ccgttatatg atttagaagt aaccaacttg aaagattcaa gtattttaca
    13081 tttatcatta gatatgatga ttgctgattt tatgagtatt aagattatat ttgaagaatt
    13141 agaatcatat tattttgagg ggaaggctaa agttaataaa gaactatcat atagagatat
    13201 tattgtatat tatgaaaata aaaaaatgaa tcctaatatt ataaacaaat ataataaaga
    13261 tgaagaatat tggaaaaatc gtattaatag tttaccagaa gctcctgttc ttcctataaa
    13321 agaaaatagt aataaatttt ctataaaaca atacaatgaa tttattaata aaaaaagaaa
    13381 atttgagttg gaaaaaatgt gcaaggaata tggtataact gtttcaagtt ttattttggg
    13441 agtttatact gaggttttaa gatattggtc tcaacaaaag aatttttgta ttaatgttac
    13501 catatctgat agagaagata ttcatccaga ggttaaaact acaataggag attttactgt
    13561 tgtcaacata ctagaaatat cggatgaata taaattaagt tttattgaga gagtaaaaaa
    13621 gatacaatta caattatgga atgatatgga acattcgtca tattcaggtg ttgaagtttt
    13681 aagagaaatg tcaagggcta ggaacaagaa agttgttata ccgtatgtat acactagtgc
    13741 tcttggaatt caggataata atgaatttga aaaaattagt aaatatggag aacttatata
    13801 taaaattagt cagacaccac aagtattgat tgattgtcaa gctatacaat ataaagatgg
    13861 tatattagta aattgggatg ttagagaaaa tgattttcca gatggattaa tagattctgc
    13921 atttgaagtt ttttcaaata tattaaaaaa tttagaaatt agaaagttga ttaacgataa
    13981 aaatattatt aatttaccta atagcttgag aaaaaataga aataaaataa atgatactta
    14041 taaaaatttt gaaagaagaa ctcttgttaa gggattttgt gataatgtta agagtcgacc
    14101 agaagcagag gcaatttctt ttaatggagt ttcatactct tatagtgatc ttgctgatta
    14161 tgttgtaaca gttcaaaatg aattaataaa aagaggttgt aagcctggtg atattgtagc
    14221 tatacactta aacaaaggaa tatggcaaat agcagctaca ttaggagctt tgatggttgg
    14281 tgctatttat ttgccaatag atataaatca accaatttct agaatagata gaatatgtag
    14341 tgattcaaag gcgagatttg ttttaacttc actaaataat agattaggcc aaaaaatata
    14401 ttcaaatgaa atatgtaaag tttatattga aaatttaaag gtttcgaaag gacttgaaat
    14461 caagaaagtg ggctgcaatc cagatgatat tgcctatgta atatatacat caggaagtac
    14521 aggcaatcct aaaggggttg taattactca taaagctgca atgaatacga taaatgatat
    14581 catacaaagg tttaatataa cagaaaaaga tagagttata tgtatttcta atcttgcatt
    14641 tgatttatca gtttttgata tttttggtat tttaactgct ggaggaacat taataatacc
    14701 aagtgataat aaaaatgtat cagagtggaa ttatttgtta aaaaaagaaa aagtaacgat
    14761 atggaatact gctcctgcac aaatgcaaat gataacttct tatttagaat cagagaagat
    14821 taatggttca gatagtcttc gtgttgtcat gttatctggt gattggatac cattatcttt
    14881 acccaatgaa ataaaaagtt tatttaagaa ttcaagaata attagtttgg gaggagcaac
    14941 agaagcaagt atatggtcta tatttcatga aatagatata gataataatt ataaaagaag
    15001 tataacctat ggaactccat tagccaatca acaattctat gtgcttgact ctaatttgaa
    15061 tgagtgtcct gattgggtaa gaggaacttt atatatagga ggaaaaggat tagcaaatgg
    15121 atatcttaat gatagagatt taacagcaca aaaatttatt ttccataata atttaggaaa
    15181 aagactttac aatactggtg attgtggaag atattatcca aatggggata ttgagttttt
    15241 aggaagagaa gatggacaag taaaaataag aggtaatcga gtagaattaa atgaaataga
    15301 agctatatta aatacatttc ccggtattgc taactctaca gttgttatat caggtgaaaa
    15361 tgatgatatt attggagcat ttatacaacc tgatataagt tcatattgta aggaatcaaa
    15421 tgttaatcag aggataagaa caaaaaatga actcttattt aaaaacaaag aaaaatatgg
    15481 aatagttaag caatttaatg aatggattga agcatctaat aatactgcta tattagatat
    15541 tttaaaaaca tttcaaagta ttggtatatt tacagacttt aaaatatggc atacagagaa
    15601 tgagattata aataaaatag gaattaaaag ttcatacata tctttattaa gacgttggtt
    15661 aaaagtactt gaaaatgagc gtattattga aaaaaatgaa gaagatggat ataaagctat
    15721 aatgtatgta gatgggaatg aagctgataa tgaatgggca aaatggacaa aattagaaga
    15781 gaaggttcat tatagtgaag ttatgatgaa atatttttcg gaatctagaa agaatctgat
    15841 tcctttattg agaggagaga ttgatcctat tgaattattt tttccgggag gaaaatttga
    15901 tattgcttta gaggcatata aatataataa agttaattct tttatgaata aaactataat
    15961 tgataatatt aaattaattt tggatgattt tatgaaaata gattctaaaa ataagtttaa
    16021 aatattagaa gttggagctg gagtaggggg agttagccaa gagttaatag aatctataaa
    16081 agaatatcca gttgaatata tgtttacaga tatttctcag tcatttttaa attttgctcg
    16141 tagtaacttt aaacaatatc catgggttga atttcaattg tatgatataa acagaaatta
    16201 ttgggatcag aatataaatt catcctcata tgatttaata ttatgtaata atgtgcttca
    16261 taatgcaagt gatttattac gtgtaatgaa acagattaaa gaaatggtga aacctaatgg
    16321 atatattgta attttagaag aaacagagaa taattataca ttattaacat cggttgaatt
    16381 tttaaatgga tttaacaatg taaaagattt tagaaatgaa aataatcaag tttttttgga
    16441 taaaaatcag tggggaacaa ttatagagaa aataggagga aatattgtat cgattttacc
    16501 aaagattaat gaatctttaa atggatttgg acaaacatta tttgttatac aaattgaatc
    16561 taaaataaaa tcattaaatg aaaataaaat aaaatcatat ttgaaacaaa atctacctga
    16621 atatatgatt ccaaatcata tcgaaatatt aaaagagttg cctataactg taaatgggaa
    16681 aatagataga aaagctctta tgaatagagt taaaagtgaa aaagaagaag tacaaaataa
    16741 aacagaaaaa atacaattaa aaactgattt agagaaaaga gtagcagaga tttggaaatt
    16801 aatcttaaaa agaagggata tttatgcaaa tgaaaatttc tatgaaatgg gaggagattc
    16861 tttagttgca gctcaattag ttgcccagtt aaaagaaaat atagttgaag caaaagagtg
    16921 gccttgggat aagttaatgg ttgcattaat tgaatcacct acagttggag ggttgtgtaa
    16981 aagacttata gaaaaacaaa gtacaatgtc ttcaaagaaa gaaaataaga tgagtggatt
    17041 aatttactta aaagaggatc caaatagttc aataactaga gtattaatac acgatggaac
    17101 aggaactata aacccttatg ataaacttat accactaatg aaagaacttg atggtgattt
    17161 agtagctata atatgtgata atactgaaaa atatttatct ataaaatcag atgagttaat
    17221 tcaaacatta ggaataaaat atgcaaaaaa gttaatagaa tctagcgaaa aagattttga
    17281 actaataggt ttttgtattg gaggattaat tgctttagaa acggcaaaag ttttatctga
    17341 aaatggaaag aaagtaacta ggttaattag tatagataca actccgagta aaaaaatgat
    17401 atataatgaa ttacttatgg aaagagcatt tgggatgatt ataggttcaa atgtatatag
    17461 agcagggcat atggtagaag attttgaatt aaaaaaagct attttatcac taggtgaaaa
    17521 atacggatat ggatctgtaa tttcaaacga agaattatta gtgatgaaag aggaattcaa
    17581 taatgttata aagtgctatg agaagttagc cttgatagat aaaaaagagc gttttaagaa
    17641 aatttacagc aatatctata aatatggaga taaaaatatt atttataatg aagaacagtt
    17701 accaccaata tatgaagttt tttgtcatag ttttaaagct atgatatctt ataatccagg
    17761 agtgtacttt ggaaaaactg taattttaga ttgtagtaat caaaaaacaa cttttttacc
    17821 aattgatgat tgcgataacg aacttttttg gaaaaatgtt attataggag aatcttcaag
    17881 agttaggata gaaggaaatc atctaacatg tttgcaagaa tcatatgtaa agaatgtgtt
    17941 tcatttatta tataaggaga tgtaattatg aaaagaaagg ttggcatttt aggttacggt
    18001 ggtaaagtag ggcaaatagt tgttgatatt ttaaaagaaa agtatttatt aaaatgtgga
    18061 caacgaagta tatcagaaga tatttatatt aactcagatg acacaataga atattgcaaa
    18121 attgatatat gtaatcatga aatgttaaaa gagttttgtt tagattgtga tcttttaatt
    18181 aattgtacag gaccatcata ttttacatca tgtaaaatag ctgaagaaat tcaaaaatat
    18241 gatataaaat ttgtagatat ttttggagtt actttattaa ataataaaaa ggctttatct
    18301 aacttaacag gggttatagg agcagggagt tttccaggat tttcagcaat tttagtctca
    18361 tggatagctg agcgttatgg aaaaagtatt gaaaatttat ctatttatgc gggagggaaa
    18421 gagcttataa caacaaatgc ttgtgttgat gttttaatga gtatggttga aaattttggt
    18481 aaaacggata gatatatttt taatggaagc gtatctataa atgaaagaaa accaaatgaa
    18541 tcaaagttat ttaatatatt tggtagaaaa gcatatatta atgaatattt aacggatgag
    18601 atatacaatg ctggaattaa atatggaatt aaggaattac attggtataa tgttcaaata
    18661 aatccgttat ataaaacagt aatggataga gctgtgatgg atttagctaa aattaaatca
    18721 aaagatgata tttatcaagt tgcggaaaaa ataaagatag atttagaaaa aagtaattat
    18781 aattcaaata attggtataa atattggatt gaaataacta taaataatgg atttggaaaa
    18841 tatttagaga atatagaggt ggtgtgtagt gatacatata aaattaatgg aataatagca
    18901 gctatgtgtg tagagagatt gttaaataat aagtatcata aaaatattta ttgggcattt
    18961 gatttgttgg atagcgaaat agtaataaaa aacttggttt caaatggagc tataactgat
    19021 ataaaagttg gtaatgagag tataatgaat aaattattta ctgaaataga ggagggagag
    19081 atttgaatga taaagtaaaa ctaaaaattt tagtttgcgg agttggtttt ggtcaatttt
    19141 atctgaaggc actggaaaga ttaaaagatg aatatgaatt agtaggtatt ttttctagag
    19201 gtagtgaaat ttctaaaaga tatgcaaaaa aatataaagt accattatta acagacataa
    19261 gcggtataag caaaaatgat gttgatgtag cttgtgttgt aattaattca actctttttg
    19321 gaggcaaggg aagtcatata gttgaagatt ttttaaaaaa aggaattaat gttatccaag
    19381 aacaacctgt tcatatagat gattatataa attttttaaa acttgcacaa ctgcataagt
    19441 gtaaatataa attaaacact ttttatcctg atttaagaat agtagataat ttaataaaaa
    19501 tagttaaaaa actaaatgat tattctgaat taagatatat tacagcagaa tgtagtgttc
    19561 atgttttatt tccacttatt gatatattag gaagagcatt aaatggtata aaaccttgga
    19621 aattcaaaaa aatatctgat gtaaaaacag actcaccatt ttgtattgtt tcaggatata
    19681 ttaatgatat tccaatatgc ataaatattc aaaatcaaat tgtatctaaa aatcctgata
    19741 attatatgtt attacttcat agaattggat tcactacaaa ttgtgggaat ttgttaatga
    19801 ctggaaccaa tggggttatt atatgggagc cttgcattga aaaacagacg aatgaagaag
    19861 gtaaatttga tttagaagta caaaatgaat tttcaaaatt aaatacattt gaaattgtaa
    19921 ataaacaaga aaatattaca ttttatgata tgatgctagg agattgggtt aatggattag
    19981 ttagttcagt tttagaattt aaagatgaaa taataaagga aaaggaagtt ggtaataatg
    20041 agcaatattt aattgcagta atacaagcat ggcatgatct ggctatagaa ataggtagac
    20101 caactataat taatgaatat tataaaagtc ctttaagttt gaaagaatta atgaaatttt
    20161 aactatgtat aagaaaatat taggaggtaa tttatgtata aaaataaagg attaacaata
    20221 aagaatatgg taacaattgg tgtttttagt gcgatttatt gtgtatttta cataattgga
    20281 ggaattccat ttggaataaa tccggtatta acattttatc aagcaatggg cagtgcttta
    20341 ttgtgtggac ctgtatatat gcttttggtg gctaaagtag ggaaaaaatg gagtgttttt
    20401 attttgggaa ttgttgttgg aattctccta tttattacag gtatgcattg ggctatggta
    20461 ttaggttgta ttattatggg atttattgca gacataatag ctagaattgg gaattttaaa
    20521 agtaaaaaaa tgaatattat ttcatatatg atattttctc ttggaggagt aggaagtttt
    20581 ttagcatatt ttataaatcc aaatggatgg attagagcaa tggtagagaa aggtacggat
    20641 caatcatatc ttgatacaat gaatgcggta gctaaacctt ggatgattta tgtaatcatt
    20701 ataggaacac ttcttgttgc agcttttagc gcatgggttg gaagtaaatt attaaaaaaa
    20761 caatttgaaa aagctggtat tgtagactaa tgatatattt ttcaaaagag ggattaagat
    20821 ttgatcctag aataaaaatt ttaatattat ttatatgtgt aattaccgca actattactc
    20881 catctttaga ttatgaatta gaatttgtat tgttaatagg tgtatttgca atagtaaata
    20941 aaaaatggaa gtatgctcta aaaactattg ttatatatgt atttatatat tttctttcag
    21001 tatggacaat atataatgct ccaggttcgc taagaggtat acttatggca ttcttaggtc
    21061 tagttcacaa agtttatcct tgtgctatga tgtctggaat agttatatca acaacaaaag
    21121 ttagtgaatt tttagtagca atgaataaaa gtcgtatacc acagaaatta attattccaa
    21181 tagcagtaat gcttcgatat ttaccatcga tacaagaaga ttggcattac attaaagatg
    21241 caatgaaaat gagggggatt tcaccatcat taaagggatt tataactagt ccttccatta
    21301 ctgttaattg tatttatgtc ccattaatga tggctgcttc aaaggtggca gatgaactaa
    21361 caattgcgtc tgtaactaga ggaatagaaa atccgaaacc taggacttct ttgattcaaa
    21421 ttaagattaa taaagtagat atttgtgcat tattaatatt cgtattattt ctaatttggg
    21481 ggatttactt ggggaggtga tttgttaagt gattgaattt aaaaatattt cattttcgta
    21541 ttgtggtcag aaacaaggaa gtcttaagga gataaatttg catataaaaa aaggagagtg
    21601 tgtattacta tgcggaagaa gtgggtgtgg taaaactaca attattcgtt taatcaatgg
    21661 agtgattcca catttttata caggggattt ggtaggagat attttaatag aaggagaaca
    21721 tatatcagaa tttccaatgt ataaaattgc agagtatgtt ggctctgtat ttcaaaatcc
    21781 acgtactcaa ttttttaaca ttgataccga tagtgagatt gcttttggac ttgagaatat
    21841 ggcatatgat tctgagagaa tagctaatat agtagaaaaa gtagctatag atcttgatat
    21901 agaaaaactt agagggagaa gtatatttaa attatcaggt ggtgaaaaac aaaaaattgc
    21961 tttcgcatct gtttatgcaa tgaatcctga tattttttta ttagatgaac cttcatcaaa
    22021 tttggatatg atatctataa aaaatttacg taagaaatta aaaaaactaa aagataaagg
    22081 gaaaacaatt ttaattgcag aacatcggat tttttattta atggatttgg tagataagat
    22141 tatatattta gaggaaggga aaatagctaa agtgtattgt cctaaagaat ttagaaaaat
    22201 acctttagaa caaagaaaag ctatgggatt acgttctatt aatctttcag aagaaaattt
    22261 taaatataga aacctattaa atggaaaaaa agatttaaaa attaataact taacagtatt
    22321 ttataaaaaa agagatataa taaagaattg ctcttttcaa gtagataaag gagaaattat
    22381 tgcacttact gggaacaatg gcgaaggtaa aaccactttt tcacgtgttt tatgtggatt
    22441 acataaagaa tatattggtg atattttttg gggaaataaa caaattaaag aaaaagaacg
    22501 tttgaaaatt tcttatatgg ttatgcaaga tgtgaactat caattatttg cagagagtgt
    22561 acaagaagaa tgtttactag ggataaaaaa tcctgataaa aataaggtaa atgaaattct
    22621 aaaaagatta gatttatata attttaaaaa tttacatcca aataggttat caggtggaca
    22681 aaaacaaagg gttgctgtag ctgttagtat gatatgtaat aaagaaatat tgatttttga
    22741 tgaaccaact agtggtttag attatgaaag tatgattaaa gtttcaaaat tgcttaaaga
    22801 acttgctagt atgggtaaga ttatatttgt tattacccat gattatgaac ttatttgtca
    22861 agtttgtact aggatattac atttaagtgg aggaaagttt aaatatgatt taaatttaga
    22921 tgaagagaat tttaaaatta ttgaaaagat atttactaat aaagagtgag gccatagaat
    22981 tgaatataaa aagaacgagt ctgtatttaa agtctatata agaaattgaa ggaacatgtt
    23041 atttagcaat atgtatacaa atataaactt tatagtaaat gtttagttaa acaaaaaata
    23101 aaaaaagata tttttcaaca aaaaaatgtt gaaaaatatc ttttttaaat tatggcttga
    23161 ttttattatt atttttattt ttacaacata ttgattgtat atattgatcg ttggatccct
    23221 taagatcttt tggatagata tttttgtatc ttttaaacag agtagtaaat ctactatgag
    23281 agttatatcc tacagattta gcaatatctt taatatcaag atttgtagtt aaaattaaat
    23341 tttcagctat attaattctt tttctttgag tatattctgt tatactcatt tgatacttgt
    23401 gtttaaaagt atttttgagt ttggttccgc tcatcatagc tattttttct aaaagttctt
    23461 gaggaatatc aaaagttaag tgatcatcta tgtaatttgc tacgttttca attgcttttt
    23521 catctttttc agaaataggt ttaacatatt tcttttgttc ataggcattt aaggtaattg
    23581 ataaccactc ttttgcttta gcttcaaaaa ataaactagc tgaagtagaa gtcattttac
    23641 agtttaaaat agaatttgcc aatttattta tatgttttgt aattagatct cgtgtatcaa
    23701 ggaatatatt tgaaatatca tcattggtag tatctaaata tttttcaatc atattttttt
    23761 tgaaatttac accaacactg aaataaggat ggttaccatg taaaagaaaa cgtatatctg
    23821 aattatcaac attcatgata aaaactgtat ttgaagtcat attctgatag ggattaaacc
    23881 attcaccatt tgcagtaatt atataagttg atgaaaaact catataaggt tttatagtgg
    23941 gaaaggaatc tacaatcatt tcttttttta taaaaaaatc atgaatgtca ataataaaat
    24001 attctgtttc gtaaaaccag taaataccag ttatttcttc attatcaata tagaaagcat
    24061 tacctgctga agaatatttt tgacatttgg agcattctgt atatcctata tctttcagca
    24121 tattttcaaa ttttttcaaa caaaacacct ctttataaat atcatgataa gacatttatt
    24181 tttgatttta ttgtatcaca atatttgaaa tgatacaata aatgtagatt atatgatctt
    24241 taaaagtagg ctttatctaa ttttatataa aaagttgaat tttaagagaa gtttacaaaa
    24301 aaaaatatta tgtctaaatg aaataattta aagggaggaa taatatgttt aaagtttaca
    24361 gaaaactact aaaatatgtg cctgagaaaa aatatctagc atatatatca atattactta
    24421 caactgttag tactgtagtt acagttggtt catattatta tttaagtgaa tttttgaaag
    24481 gacttattgt gaataataat atatataagt ctaaagaata tgcattaatt attactggaa
    24541 tgttagcaat tggtgcaata ctttatttta tagcagtttt tatttgtcat attattgggt
    24601 ttagattgga aacgaatcta agaaaaagag gaattgatgg attgacaaag gcaagcttta
    24661 ggttctttga tttgaattca tcagggaaga taagaaaaat tatagatgat aatgcatcac
    24721 aaacacatca aattgtagct caccttattc cagataatgc aggagcagtc cttaccccta
    24781 tttttattct tatattagga ttttttatag gtattaaggt aggcgttgtt ttagtttcac
    24841 ttacaatagt atcaggaatt cttttaaaac ttatgatggg tgaacaggaa tttatgaaat
    24901 attatcaaaa agctcttgaa aatttaagta gtgaaacagt tgaatatatt agaggaatac
    24961 aggtaataaa aatatttggg acagatgtat cttcattcaa aactctttat aaagcaataa
    25021 aagattattc gaaatatgcc catgattatt cactaagttg taaaaagcca tatgtaagat
    25081 atcaatggtt tttttgtggg gtggttttaa ttacaattcc tattgtagca cttacaatgg
    25141 atatatctaa aaatcctcaa atacttgctg ttgagttgat tatgatttta tttttaagtg
    25201 gtattatttt taactctttt atgaaagtta tgtatgtatc tacgtatgct tttcaaggaa
    25261 tttcagcagt agataaatta gaagaacttt ttaatgaaat gcaaaaagat aaattaagat
    25321 ttgggaataa agataatttt aataattttg atattgaatt tgagaatgtt agttttggat
    25381 atacaaaaga tatgattata gataatttga gttttaaatt aaaacaaaat aaaagttatg
    25441 cacttgttgg agcttcaggc agtggtaaat ctacaattgc aaagttgatt tcaggttttt
    25501 ataaagttaa taaaggggtt ataaaaatag gaggtgaacc aattgaaaaa tacagagaag
    25561 aatgtttaat taaaaatatt gcttttgtat ttcaaaatgt taaattattt aagacaacaa
    25621 tatacgaaaa tgtaaaatta gctaatgaaa aagcaacaag agaagaagta ataaatgcgc
    25681 tttatttagc gggttgtggt tctattttag ataaatttaa agatagagaa aatactgtta
    25741 taggaagtaa aggggtatat ttatctggag gagaaaaaca aagaatagca atagcaagag
    25801 cgattctcaa agatgcaaat atagttatac ttgatgaagc aagtgcttcc gttgatcctg
    25861 aaaatgagca tgaacttcaa attgcatttt cgaatttgat gaaaggtaaa actgttatta
    25921 tggttgcaca tagacttaca agtatacgaa atgtagatga aattctagtt atagaagatg
    25981 gtaaaattat cgaaagaggt aacgatttag aattgatgag tatagatagt aaatacagag
    26041 aatttcaaga aacatacgga aaagctaata attggaggat aggatatgga gaattttgtt
    26101 aaaaataagt ttgcactaac agaacaggga gcaaaggatt tagtaaaagc aagttttgta
    26161 agttttttaa tttatgctat taatatggtt ccttcgattt tattaatgat atttatagat
    26221 gagcttgttt taggaaacat aaaaagtaat gaattttaca ttggattttc tataattgtt
    26281 ctggtaataa tgtatcttct attaagtatt gaatatgatt ttctttataa tgctacatat
    26341 aaagaaagtg ctaatttaag gatagatatt ggacaaagat taagtagatt acctctctca
    26401 tacttttcta aacacgattt gtctgattta tcgcaaacaa ttatgagtga tgtagaagca
    26461 atagagcatg cgatgagtca tgctatacca aaagtattag gattttatat attttttcct
    26521 ttattatcat taatgttaat tttgggaaat ttaaaacttg cttttgcagt aatattacct
    26581 gtaattttaa gttttttact tatgtatatt tctaagaaaa cacaattaaa tgaaataaaa
    26641 aaacattatt taaaattaag ggaaaattca gaatcttttc aagaaaccat tgaaatggga
    26701 caggatatta aaagctttgg tttagctgaa aaatttaaaa gaaatcttta taaaaagatg
    26761 gaagatggag agaagttaca attaaaatca aattgtgtac aagttattcc tgttgcaagt
    26821 gctggtattg ttatgtatat ttcaattgca gtagttattt taattggaac agatatgtat
    26881 atatctgggg aaattaatat tctataccta attgggtatc tattggcggc tgtgaaaata
    26941 aaagaacttg tagatggtat cactgaaaat gtgtcggaac ttttttatct tgattctaga
    27001 ataaagagaa taaaagaaat taggggaaat gaaatacaag aaggcaaaga agtaaatatt
    27061 aaaaatttta atataaaact tgaaaatgtt acttttggat ataacaacaa taataaaata
    27121 ctaaaaggaa tatcctttat tgctaaacaa ggagaagtta cagcattagt agggatgagt
    27181 ggttgtggta aaacaagtat acttagacta atatcaaggc tatatgatta tgataatggg
    27241 aaaataagta ttgggaatat tgatattaaa gatatttcta caaaggcttt atttcaaaag
    27301 atatctattg tatttcaaga tgtaacttta ttcaatactt ctattatgga aaatataaga
    27361 ataggtaaaa aaggggctac tgatgaagaa gtaagagaag ctgcttgtct tgcaaactgt
    27421 gaagaattta taaaaaaatt accacataag tataatactt taatcggcga aaatggggct
    27481 tcactttcag gtggagaaag gcaaagactt tctatagcaa gagcgttttt aaaagatgct
    27541 cctatagtta ttttagatga aattgcagca agtcttgatg tggaaaatga gaataaaatt
    27601 caggaaagtc ttaatagact tatgaaagat aggacagtaa ttataatttc tcacagatta
    27661 aaatctatag aaaatgtaga taagattgtt gttataaatg atggtaaagt tgagtgcttt
    27721 ggaaaacatt gtgagttatt ggaaaagtca aatttatata aaaagcttgt tgaggaggaa
    27781 cgattagtag aagaatttaa atattaaaat taataaagat tataaataca ctgtgattta
    27841 taaaatagat tatgatttgt aatttgaaag cat
//
LOCUS       DAKBNN010000025        27665 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000025 DAKBNN010000000
VERSION     DAKBNN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.025"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..1792)
                     /locus_tag="OUB02_001017"
     CDS             complement(<1..1792)
                     /locus_tag="OUB02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884793.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENNNNENDNSD
                     EKQPNTPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLS
                     SKSVFGTNEHSFPYRESISNKNQDTNKNKSN"
     gene            complement(1975..3450)
                     /gene="spoIVA"
                     /locus_tag="OUB02_001018"
     CDS             complement(1975..3450)
                     /gene="spoIVA"
                     /locus_tag="OUB02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HCT8884794.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3622..4917)
                     /locus_tag="OUB02_001019"
     CDS             complement(3622..4917)
                     /locus_tag="OUB02_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8884795.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5101..6510)
                     /locus_tag="OUB02_001020"
     CDS             complement(5101..6510)
                     /locus_tag="OUB02_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8884796.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(6562..7701)
                     /locus_tag="OUB02_001021"
     CDS             complement(6562..7701)
                     /locus_tag="OUB02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT8884797.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8020..8670
                     /locus_tag="OUB02_001022"
     CDS             8020..8670
                     /locus_tag="OUB02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8884798.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(8827..9846)
                     /locus_tag="OUB02_001023"
     CDS             complement(8827..9846)
                     /locus_tag="OUB02_001023"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCT8884799.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVSEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9877..10491)
                     /gene="plsY"
                     /locus_tag="OUB02_001024"
     CDS             complement(9877..10491)
                     /gene="plsY"
                     /locus_tag="OUB02_001024"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCT8884800.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10526..11845)
                     /gene="der"
                     /locus_tag="OUB02_001025"
     CDS             complement(10526..11845)
                     /gene="der"
                     /locus_tag="OUB02_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCT8884801.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(11879..13207)
                     /locus_tag="OUB02_001026"
     CDS             complement(11879..13207)
                     /locus_tag="OUB02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HCT8884802.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13445..13645)
                     /locus_tag="OUB02_001027"
     CDS             complement(13445..13645)
                     /locus_tag="OUB02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884803.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13647..14579)
                     /locus_tag="OUB02_001028"
     CDS             complement(13647..14579)
                     /locus_tag="OUB02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HCT8884804.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14624..15556)
                     /locus_tag="OUB02_001029"
     CDS             complement(14624..15556)
                     /locus_tag="OUB02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HCT8884805.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15611..16900)
                     /locus_tag="OUB02_001030"
     CDS             complement(15611..16900)
                     /locus_tag="OUB02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8884806.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYISKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(16929..17621)
                     /locus_tag="OUB02_001031"
     CDS             complement(16929..17621)
                     /locus_tag="OUB02_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884807.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17636..18397)
                     /gene="pgeF"
                     /locus_tag="OUB02_001032"
     CDS             complement(17636..18397)
                     /gene="pgeF"
                     /locus_tag="OUB02_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCT8884808.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18394..18855)
                     /gene="nrdR"
                     /locus_tag="OUB02_001033"
     CDS             complement(18394..18855)
                     /gene="nrdR"
                     /locus_tag="OUB02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCT8884809.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(18877..19140)
                     /locus_tag="OUB02_001034"
     CDS             complement(18877..19140)
                     /locus_tag="OUB02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HCT8884810.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19244..20017)
                     /gene="sigG"
                     /locus_tag="OUB02_001035"
     CDS             complement(19244..20017)
                     /gene="sigG"
                     /locus_tag="OUB02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HCT8884811.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20109..20831)
                     /gene="sigE"
                     /locus_tag="OUB02_001036"
     CDS             complement(20109..20831)
                     /gene="sigE"
                     /locus_tag="OUB02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HCT8884812.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(20856..21728)
                     /locus_tag="OUB02_001037"
     CDS             complement(20856..21728)
                     /locus_tag="OUB02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HCT8884813.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22018..23292)
                     /locus_tag="OUB02_001038"
     CDS             complement(22018..23292)
                     /locus_tag="OUB02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCT8884814.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23576..24733)
                     /gene="ftsZ"
                     /locus_tag="OUB02_001039"
     CDS             complement(23576..24733)
                     /gene="ftsZ"
                     /locus_tag="OUB02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCT8884815.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(24923..25276)
                     /locus_tag="OUB02_001040"
     CDS             complement(24923..25276)
                     /locus_tag="OUB02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HCT8884816.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25375..26049)
                     /locus_tag="OUB02_001041"
     CDS             complement(25375..26049)
                     /locus_tag="OUB02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HCT8884817.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTITGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(26054..26752)
                     /locus_tag="OUB02_001042"
     CDS             complement(26054..26752)
                     /locus_tag="OUB02_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HCT8884818.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26775..27515)
                     /locus_tag="OUB02_001043"
     CDS             complement(26775..27515)
                     /locus_tag="OUB02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HCT8884819.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         8569 a         5098 c         2854 g        11144 t
ORIGIN      
        1 tatttgactt atttttattt gtatcttgat ttttgttaga aatactttcc ctataaggaa
       61 aagaatgttc attggtacca aatactgatt ttgagcttaa atcataatta tcatcatttt
      121 ctgtaattga atttttattg taaaatcctt tttctttatc agaattattt tctttattac
      181 catctttagg tatatcatga ggatttttat tttcattgct atcagtactt gattctacat
      241 catctttagg agtatttggt tgtttctcat cagaattatc attttcatta ttattatttt
      301 cattattatt attttcatta ttattgtttt gattgttatt actatcatta ttttcatttc
      361 cattattccc atcttgattg ttatcatcct catctctatc cgggtcgtct ctacctcctc
      421 ctccacggtc accatcaata tcatcatttt cccgttcatc atctggtgtc tcttggttgt
      481 ctccgcctcc cattataaat cctggtttaa aaactatatt tgaataccct tcttttaatc
      541 ctcctacaac ttttactcta aaacaacgca acttaaaatc ttcaaaaaaa agtatagctt
      601 gatctctaat atcctcctgt tcttcagata tccaattgat tccatcgctt gaatattcaa
      661 caaaaacctt acttgcacta tacggcatat cataaaataa aacagctaca tatccatttt
      721 ttgtattttg aaattccagt tccaaattgt ctattggttt tttctctata acagatacat
      781 tcaaaatagg aactacatca tcatttatta taattttttc tttatttaac atttctgttt
      841 taaactttcc tgttatatta aaattttctc ccttttccaa cttttcataa taatcagtaa
      901 aatcccattc tacatctata tcaagtcgat taccactaga tgtttctaat accatatatt
      961 tgggaaattt tatattttct attttatcat ctttacttac ttttatttca tcactaaata
     1021 caccaattcc cctatgacaa catttcacaa tatcatagcc agaatcttca gctatagagg
     1081 gtataagctc attataatca tcaattatat aaactttctt actttctgat ataattgatt
     1141 gtgccaactt attattatct cttagtaacc cactttcatt gttgctacta tatgctttta
     1201 ttgaagtttc ttctatctca acatcacctt tagaataaac acctatagca tgttctccat
     1261 tcacttcaat atccttattt agtattctaa tatcattttc agtataaatt cctattgcat
     1321 cttttccatt tgcctttatt ttttcaacag attcgtagga tgatttaata tgtaagtctc
     1381 ctccttcttc aacgtataag gctgtcccat cagtagcaat tatgttgttt ataccaatac
     1441 ttatacttcc accattttct atatgtatta ctccttcttt tcctccttca cctattatat
     1501 tcaattcatt atcatcaata ataaatctcc catgattttt tatcaatatt ttatatttgt
     1561 tggtatctat tgtaaaatta ctgtcataag gaatataaaa tctaaactct tcatctatta
     1621 ccatatcaca agaaagagta taaactattt cttctttatt atttttattt tcttgaaccc
     1681 acctttcaaa atcttcaaga caattttcct cttgtccaaa aactttatgt ggaaaactta
     1741 taaaacacaa aactattata ctcataaaaa taaatatctc atactttttc atcttacact
     1801 cctagaaatt tttagctttg aaacaatcct gtcgaaacat atacttcacc cactataatt
     1861 tttccacaat tatcaattat tgtcaattta tataagtatc tttattaatt gttataaata
     1921 aaaagctact tactaaagca aacagacgtt tcactttaat aagtagctat atttttataa
     1981 caaaatagtt ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat
     2041 tttaactctt atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa
     2101 gtcatgtagt gatttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa
     2161 tacttccata agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga
     2221 aacctctgta gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt
     2281 gataccatat tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa
     2341 ggaaggagct actactccat atccttttat cttggcatct attaaagcac tttctatttt
     2401 atcatactca tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc
     2461 ttcaatttta aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg
     2521 taaatcaata tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa
     2581 aaactctaac tcagaaaatc cttgtataat accttctata tcccttattt tacctatatc
     2641 tataatactt tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag
     2701 accttcaacc cattttggta agttgattct tatttctgta agtggaaaat catataatac
     2761 agcttccata acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac
     2821 tggaacttca tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg
     2881 acttaacgta tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac
     2941 tctctcttct ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa
     3001 gacaactatc cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc
     3061 cttttcaaat gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc
     3121 ttcctcatgc cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt
     3181 taaagatact gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc
     3241 tactgtcatt atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt
     3301 tttaaattca ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt
     3361 tgattttcct gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt
     3421 ggatatatct tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta
     3481 aattatattt agagttaagg gttaatattc ttaaaataga aaaataaaga ccagcaatat
     3541 taatttaatt tacatataga aatttgtaaa tttcaatgta aattaaatta atattgctgg
     3601 tccaaatagt agtgtattat attaactgac tttagtagta ttttcatttg aattattatt
     3661 catctcaata agttctttac gtctttcttt tgtcatgttc atccatataa atgtaaacac
     3721 caatccaatt atggctacta tagtaagaac tatgaagtag tacctatatc cagttacacc
     3781 cggaaatgca tccaatatct tccctgctgc taaaggacaa agtacttctg gaagatatcc
     3841 taatgtagca actattccta tagcagttcc tgatattgat aatgaataat taccttcttc
     3901 aagtaacgaa tagtgtaaac cttgaattgc atacatagat acatatattg caacacatcc
     3961 aatcaatagt atataaatca tagatgattt tgttggtgta aatattacac ctaaaagacc
     4021 tgcaatcata agtataaatc cataagatat aatctttgat gaaccaactt tatctccaag
     4081 taatccagaa gcagctgatg ctactggtct acaatactga gctaatattg agattgcaga
     4141 tgcaaacacc acagtagctc caaatgcttc agttgaataa ggcgtaaaat aataaaatga
     4201 catattagct gaatatgaac aaaacattat tattgttatt atccatgtat gaggcatttt
     4261 tattactgca aaaaaggctt ctttatcaaa ttttgctgat acctcttttt cattatcctt
     4321 taatttgaaa aatactaata ctccaactat tacatttatt gccgagtata atactataat
     4381 agctgtaaga ccagatttat cacttacttt tgatgaaata tacccaaata gtatcaatac
     4441 tagagataga tgaattgcat tggtaattcc acgaccactt tccataaatc caaaagcttt
     4501 tccttgttca ttttcagatg ctaacattct aacagctttt acaagagctg gccaaaaagt
     4561 aagtattgtt gttattcccc ataaggcatg gattaaaaac acaacaatat atggaggata
     4621 aagtgttaat acaagaccag atattcctgt aataattaaa gaacctgaaa gcaattttct
     4681 ggctgaaata cggtctgcta aaaaaccacc aaaaaggtat gataccattg caagtagacc
     4741 ataaatactt cctaagcttc ccatttgtgt atttgtaaga ttaaatgctt gtgcaaaagt
     4801 atcataataa taattcttta aatatggtaa agcatatata aatgctcctg ctacaaagag
     4861 tattatcaag actttgaaat ttttccaaat gcttggttta tttacttcaa ctgacatact
     4921 tcctccttat gtaaatacct gtaaattttc atgataatta attattgctt attgtattgt
     4981 ttattcaata tttcggaata tttattaata tattaggtta atctatcaat aatttatggt
     5041 gagttatgag ctaaaagtaa actcttttag ctcaccataa taattatatt tttatttata
     5101 ctaacgtcca gagttattag atatcttttt ttcatagttc ttccatagca ttaaacctgt
     5161 tattataaca aaaagacctc ctcctatcat ttcaatctca tagattatat tatcttttaa
     5221 agtctccata actggtgccg ttgagaaaag tatgccaact acagttacta caagtaccat
     5281 tctggcaata ttgatacaaa ctgaatcact ttttgcaatt gaatatggtc tttcttcatt
     5341 tggtcttgtc tttctaagtc taatataaga tgtaaaaagc aagacatatg gaaataatgc
     5401 tgttaatgct gtcatagtaa caagaacatt gtatatatta tctactgatg gtaataagtt
     5461 tgttcctaca agaagtagac tcactattat agcctgaact attactgcat ttgatggtat
     5521 attgtattta tttgtaacag tcaattgctt aggaaataca ccttctctta catttccaaa
     5581 aagcatcttt actggacctg ctatataaag tattaatgct cctagtactg ataaagttat
     5641 tccaaatgct actattctta taaaccaact tcctatacct aaatcttgtg caactttagc
     5701 taatgcatct agtatccctg tagatgctgc aattttatct ggaggaagta tcattgttat
     5761 tgctactgaa cctaagatat agattcctcc tattaaaaca gccacagtta atatagcttt
     5821 aggaaagttt ttcttagcat tatccatttc ggttatgaaa ttagcagtcg tttcagctcc
     5881 tgataatgca aatattatag cagatataga aactaatgaa ttggcatcta tttttggtat
     5941 tatattttgg attgtatata tcgatgctga tggagctttt ttgagaataa ctacagacat
     6001 aaatgccatc actataagta atatagcagg aattgtagaa cctaatgctc ctgtattcgt
     6061 aaaaaatttt ccaaatgcca tcccacgagt acttactaag gacaacagcc aaaatattat
     6121 gagtgaaagt gctaaaataa acatcttatt gtttgataaa tctggatttc caagcatata
     6181 tgcaatattt atcgctaaaa aagtcaagaa agaagaatac caaaatatct ttgatgtcca
     6241 atttagccat gacaccataa atccccactt ctctccataa gcttgtttaa cccattctcc
     6301 aagtccacca tcttttggat atgttgcagc caactctgca catataagtg atgcaggaac
     6361 aaagaatata aaagcaaaca ataaccaaac tggtatagaa ccaagaccca atccaaaact
     6421 agaggcagta gatttagcta tccatctaat cccaaatagt gctgatatat tcataaatac
     6481 taaatcccaa aatccaattt tctttttcac tgtaattcct ccaatttcaa atttattcag
     6541 actcatacac aatgtattga cttaaatatc aagtataagt tcttcaacac ttcttggata
     6601 attagttata attgtagcac cattcttacc tactattgct gtatctgagt gtctaaatcc
     6661 tccaactcca ggtatatata tccctggctc catagaaaat accattcctt cttctaatat
     6721 taattcattg tcaaatctta aatatggctc ttcatgttct gataaaccta aaccatgacc
     6781 aattctatga tttgaatata actctagacc atactttgca acaacagctc ttgctgcttc
     6841 atccaccatt cttgctggta taccagcttt tattgtatct aatccagctt gttgagcctc
     6901 tactgcaatc ttaaacactt ctttttgtct ttctgttggt ttgcctataa taaaagttct
     6961 ttcattttct gctctatagt tttcatacca aacctgtcta ctatgtatta ctatatctcc
     7021 tctttgaagt attctagtat tggaatctaa atgaggttgt gctgttctat caatccctga
     7081 acaagtccag tttgcaaatc ctatatatgt atcaggataa ttttcagaaa catattttag
     7141 taaagcatta tcccctgcaa cgtcaaactc caattcactc atcccaactc taacattttc
     7201 taaagaccct tttattccta tatcagatag gtaacctgct atcttcaaaa attctatttc
     7261 ttttgcatct tttacatatc tcatttcaaa tattttactt ccaacatctt tgatagcaaa
     7321 tgacttatca gttatatact ctgaaaagct tgcagatact atatcttttt ctattccaac
     7381 agttgttcct ttagggtatt tatttaaaat tatatctaag taatgtttat gtgatattcc
     7441 atgttctttc atttctggta tttcataata cacatataca ttatcaactt ttgcaacttc
     7501 tttagcatgt aattcttcta atccaggtac tattaattct attttatctt gagttactac
     7561 tgttacaatt ggtctagaat aagttattgc tttaaatcca ctaatataaa attgattgtc
     7621 tggttcaaat attaaagcaa tttcaatgct tttttcattc atgtactttc tcaatctttc
     7681 tagtctcata ttatttgaca taatgcacct ctttcttaat attcagataa tttattcaat
     7741 acaacaagga tgaatatatt attttaaaat tcttaatatt tttagtactt gtcgtatttg
     7801 tatgtaaatt aattttattt gtgattattt tatcataatc atgattattt tgtcaatcat
     7861 gattttattt taaaaaattt tttatattct agtgatttta aattatctaa aatataacga
     7921 gtatacattg tattttgtaa tattattctt gatttatggt aagtttaatg ataaaatcac
     7981 gattatataa actatattga ttatttgtag gtgaataaaa tgacaaataa agaattacaa
     8041 aagaaaagga tattgatgta tttcatagaa gctgctcaaa atattatgga aaacgaaggt
     8101 atagaaaata ttacacttcg taaggttgca gatatggctg gctataatag ttcaactcta
     8161 tataaatact ttaaaaattt agaccattta atatcatttg catctataaa atattttaaa
     8221 gattataact tgaatatttc aagatgtata gaaaatgtca atgatgaata taagaaatat
     8281 ataattatgt ggaaattatt ttgtaagcat tcttttgata atgctcaagc tttttaccag
     8341 gtatttttta acttgtctag tgatgaactt agctatataa caaaacaata ttatgatatg
     8401 tttccagaag atttaggtat tcatgatagt gacatatctt taatgattac aggagaaagt
     8461 ataaaggaaa gaaataagat actattaaac aaccttgtag atactggata tataccagct
     8521 aaagatttag acataatcaa tgatataatt atttcttttt atcaaaatct attattctta
     8581 aaaaagaaca caggaaaaaa ttctgatgat gatgaattaa ctatgaaaat gatttcttct
     8641 attgaataca taataaaaca tgctaaataa aattaaaata tggctgtctc aaaatagaga
     8701 taagcctttt ttattgttga aataataaaa atatatttaa tttcatataa aaaagagtgc
     8761 ctcgtaaact aaaaagttca ttttgagaca cccctaaaat aaatttatta tttattatac
     8821 aatagtttat atatcatcac ctaaaactac ttcttccatt tcatgctttt tacttctcat
     8881 cattagttct attccaactt ctttaggatt tgaacctttc tttattacag aatatatagc
     8941 attagttata ggcatatcta tatctaattt ttcagaaaca tcatgtgcta cttcagtagc
     9001 agttatgcct tctacaacca tctttacttc ttttaaagta tcttctaaac tcataccttt
     9061 acctatgagt ataccagctc ttctatttct actatgcata cttgtacaag taactattaa
     9121 atctccaata cctgaaagac ctgcaaaggt agacatatta gctcccatag caattccaag
     9181 tctgctcatt tcactaatac ctcttgtcat aagggcagct tttgcattat caccataccc
     9241 taatccgtca catatcccag ctccaaatgc tataatattt tttaatgctc ctccaagttc
     9301 tactccaact atgtctggat tcgtataaac tctaagttta gggctcataa ataaatcttg
     9361 tatcatctgt gctattttta aatctttaga agcaactact acggtagtag gaatatccct
     9421 tgctacttct tcagcatgag atggcccaga tagtattaca tatggatttt gtggtaattc
     9481 ttcttcacag acttgagata atctaagccc agtccctttt tcaagacctt tagctacatt
     9541 aacaataact tgtccttctt ttataaatgg ttttattttt tggcaagtac ttcttacagc
     9601 ctgtgatgga actgctaaaa ctatcacctt acaatctttt atagtttttt ctatatcagt
     9661 acttatagtt atattatttg gaaataatac tcctggtaaa tagtctgtat tttctttagt
     9721 tctgtttatt tttttagctt gttcctcttt tcgagtccac atgctcacat cattaccttt
     9781 ttttgcaagc cctaaagcta atgcacttcc ccagcttcca gtacctaata cacatacttt
     9841 ctccatgttt aacaccacct gagtataata tatttactat tctttctttt gaccaatttt
     9901 tctctctgtc ccatttaata atcgctttat atttgctctg tgattatata ttactgatgc
     9961 agttaagaac agagctacta ttaaaccagc tttatttttt accataatca taaatattgg
    10021 tgaacatcct attcctacaa cagaacccaa tgaaacatac tttgttatag ctaccaccaa
    10081 tataaatact gctagacaca ttagtgttat tactggattt acagctagca ttgcccctag
    10141 tgaagttgca acacctttgc ctcctctaaa tcctaaaacc gctggccaat tatgacctgc
    10201 tacaacacat ataactgcca gatatccagc taaaagagta tctattccaa ccaatcttgc
    10261 tgcaaattct gatattaaaa cagccattac acctttaaga acatcaccta aaaatgtcat
    10321 tgctcctgct ctttttccaa gtgtcctaag tacatttgtc gaacctgcat taccagaacc
    10381 ttgagtcctt atatcaaccc cagcaattcg ctttgcaact atgtatgagg tagatatatt
    10441 tcctaaaaga tatgcaacta cagcaataat tatataacta aatatctcca ctgaatttcc
    10501 cccctttagt tatattttaa gatatttatt ttttattttt ttgtctatac tcaaacttta
    10561 tggaagttcc ttcaaatcca aaattttctc ttatcttatt ctcaaggtat ctttgatatg
    10621 agaagtgagt taaatcctta tcatttataa acaatgtaat tttaggaggt ctaatgtcag
    10681 tttgagtccc atagtaaatt tttaatcttc tacctttgtc agatggtggt tgatttaaca
    10741 ttacagcttc tccaattact tcattcagtg ctgaagttga tattctctta gaatgttcat
    10801 ttgacacata ttcaactgta tctagtatct tgttcattct ttgattagtt tttgcagaaa
    10861 caaatacgat tggagcatac atcataaatg gaaatttttc ttttatatct tttgtgtaat
    10921 tactcatagt cttattatct ttctctatta aatcccattt attgattaca aatatgcatc
    10981 cttttccttc atcatgtgct atacctgcaa cctttgtatc ttgttctgtt acaccttcta
    11041 atgcatctat tactatcaat actacatctg ctctatctac agcactcata gctcttatta
    11101 cactgtattt ttctattgtc tcatatattt tactttttct tctaagccct gctgtatcta
    11161 ttagaagaaa tttctgtcca tttttttcaa aataagtatc aattgcgtct ctagtagttc
    11221 ctgcaattgg acttacaatt actctttctt ctccaagtat cttattgagt atagaacttt
    11281 ttcctgcatt tggtttacct gttatagcta ctcttattat atcttcttca tactctgtat
    11341 ctaatcctgc tggaaaattt tcaactatct catctaataa atctccaaat cccatactat
    11401 tggctcctga aactgcaaaa ggcgttccaa atcccaactc ataaaaatcg tatatattat
    11461 caaattgact ttgactatca atcttattaa ccactaagat tactggtttt tttgtctttc
    11521 taagcaattg tgcaatttct ttatcagcag ctgttattcc agctttacca tcaactataa
    11581 aaagtattac atgtgacatg tccattgcaa gcatggcttg atttctcatt tgagataata
    11641 ttatatcttc gctatcaggt tcaattcctc ctgtatccac taatgtaaaa tatttatcta
    11701 accattctac ttcagcaaat attctatctc ttgtaactcc aggtgtattt tcaactattg
    11761 atatcctttt tcctgcaaat ttattaaata ttgtagactt accaacgttt ggtctcccaa
    11821 ctacagctac aactggccta cttatactca ttttatttct ccttccatct tttgttttct
    11881 attttaaaat tttatccaca aaatcttttc cttcattcaa acaagggata atttttattc
    11941 tcataagttc ttctaattga tttaattcaa tattatctaa gaaaatttct tcatctgctt
    12001 ttagcatact tcttgttata taaagagctt ctcctaattc tttatcttca agttgttctt
    12061 ttaaatcctt agctgttaaa agacctgata cagttattgt ctctccaaaa aacttgtttt
    12121 tgatttcata tacatttact tgaagatttt taaacttgtc catcatagca tctgccatac
    12181 tctgtataaa ttcatatgca gaatgaccag tagctattga tactttcttc tctcttttta
    12241 gaatattctc aggcaatata tttagatact cttcaatctc tgtttttaac ttacgtatca
    12301 tacctacacc atcttcaaac tgtaaaaaac cttcatattc ttcatatcca ggcaattcac
    12361 tattagatag tatataaaat tcatctgata aaaatgcaaa tcgagtacct aatttttcaa
    12421 gatatttctg ttgtatttga tgtatttgct ctatagtttt acttgcgctc ttatcattaa
    12481 atatatttaa ttctactaga ttttctctat gctttgtaat cccaactgga actatcgcaa
    12541 cactgtttac atatggataa agttgagcta gttcttttat tgttctatcc aattcctttc
    12601 catcatttat acctggacat aaaacaattt gacagttcat ttctatatga gcatctgcca
    12661 atctcttcat tataccatat aattttcctg caaatttatt gcttatcatt ttttgtctta
    12721 actctggatt agttgtatgc acagatatat ttataggact tattctatat tttataatat
    12781 tatttatatc ttcttcactc atatttgtaa gtgtgacaaa atttccttgt aaaaaagata
    12841 atctcgaatc atcatcttta aaataaagag tttctctcat accttctggc aattggtcta
    12901 taaaacaaaa catacactta tttctacagc ttttagcttg gtcaattatc ggatttgtaa
    12961 attcaacacc caaatcttca tcatagtctt tttctatctc gtatatgtag acttcgccat
    13021 cttttttttg tatttctaca tctaaatatt catcacttag taagaatcta tactctatta
    13081 tatcatgtat tggctgttca ttcacagata taagtaaatc tcctacctct atacccattt
    13141 cttctgcaat actatcttta taaaccttac ttataatatt atttactttt tttacattta
    13201 cttccaatat tttcaccact cttttgtatt catcaatgct tattataatg tattttaata
    13261 tgcaatttat ttatttgtta tattttatga tagcattttt aagcatttca tgtcataata
    13321 acacatttta attctaatag tcaatataag taaatagcta taaaatagat tagaacaaat
    13381 aatattcaaa tttaagtttc ttaaaaattt gaatattatt tgttcttaaa aaaattttat
    13441 aaatttaatg ttttactatg attaacgtcc catttgtcat atcatgaacc attccaccta
    13501 cacactttga catcaacaat gccttatttt gtaagtcttt ctcatcattt gcataaaata
    13561 ttggacaacc tccaccattt ttcatattct tatccaacgt aactatagct agtgtatatt
    13621 catttaaacc tatatccatt cccatcttat gcacttcctt tctttcctat tttataattt
    13681 tttagtgata agttttttcc tttagaactt gaaagtattg ggcatgactt tacggcttcc
    13741 attacttttt ccacattctt atctactggt aaataagcaa tcattatact ttcatcctct
    13801 ttattccttc taggtaatgg tacgaatgaa ggttctccat aatctccata taaacctaac
    13861 cttgaatata tattgtatac tattgcctgt ctttgacctg agtcaaataa tatccctgca
    13921 ttagcataat ctttattttt aggaattatt tgtattccta cacctctatt aatatatact
    13981 tctctatcac tttttagacc tatatttgtt attcccttta aatctcctac cttcaatata
    14041 gactcatcta caaaactaat ctcagcaggc attacatcag ctatgtctcc aatacttttt
    14101 ctagtaagaa gtttttttaa tataaaggct aacactaatc cacaaattga acttattacc
    14161 aaactcattc cttcacttat tttcgtttct gcaagtatta cataatacaa tcctattgtt
    14221 aaaaaagatg taactataca catataattt cttacttcat aggttctagc tatctcttct
    14281 atgaatgcag ctcctctctt tacaagttga acttcctcta agttttgcaa tgtatttctt
    14341 ctatttgctc tcacttgtct aaattgctct gctgctaggg ataaaaaagt tatagatgta
    14401 taagaacgat ttataagagc tggaaccaat aatgctccta atgaagaagc gacgaatgct
    14461 aatataagtt gagatagtaa aacatttggt tgcgttggat actgtttttg gtctaaattc
    14521 aacattataa ttctagatgt tataccaata ataacagcaa caaaaaatac tctatccatt
    14581 aaatcactcc taaaatattt aatttgaaat tttacatgta aatctacaaa gtctttctag
    14641 aaaaatcact ctgctttcct tttgatgttt ctatgatagg tgtacttttc actacatcta
    14701 tcatgacatc tatatctttg agtattggca tatatggtat aaccacagta cttttttcta
    14761 aattggtctt agatatagca acaatatcaa cctcatctac atctctatct atccccatat
    14821 gaataaatag attatgtatt atagcttgtc tttgccctat atcatttact attccaaatg
    14881 cacctaaatc tcttggtata acttctatag ccagacattc atttctatac ttttctcttg
    14941 tactttctag acctatgtta gttattatta caccatttac cattaaaata ggtccttcaa
    15001 aacttatttt tgcaggaact atatctgcta tatctgctat actgttcctt cttaaaaatc
    15061 ttctaaaaat caaaccaaca atagctcctg acacaatagc taagattgtg caataaaaaa
    15121 agctaaggcc atactttctt gcaaaaacga tataaactat cgaagatacc agtgcagaaa
    15181 aaagacttat ataacttcta gattcatacg tagatgctat ttcttcaacg taagcgtctc
    15241 cttttggtac taattcttca ttatcgatat tctttagtgt tattctttct tgttctgcaa
    15301 gaccttgaaa ctgttgtatt gctactgcaa aaaatgtcaa ggctgcaaac tccttatcta
    15361 taagtgcagg taatgctata gcacctaacg aagctgaaag cccagatata attatctgtt
    15421 ctaaataatc ttgtggtctt gatgggtatt gtttgtctgt aactcttaaa actaatcctc
    15481 tacataatat tcctacaact aatgcaacta taaaagagtg tctaaataaa tttgcattca
    15541 ataaactttc ttccattttc tcagctcctt ctcttatata gtttgtgcaa aaaacaaaaa
    15601 aaattccctt ctatttaagg aaattttttt gtaatattgt aactataaat tcgctaccaa
    15661 tgccaagttc actttttact tttatacatc catttagtga tttgactata tgctttgtta
    15721 tagccaatcc taaacccgtt cctcctacat ccctacttct tgctttatct accctataaa
    15781 atcgttcaaa tatgcgttct acatcttctt ttggtatccc tacaccattg tctataactt
    15841 taattacgat tttttcttca tcttttaaca ctacaacttg tacaatccca ttatcttggg
    15901 tatactttat agcattatca actaggttta agaacacttg ctttatatag tctttattgg
    15961 atagtatact tattgattta tcatcacatt tatataaaac ttctatattt ttttcactag
    16021 ctgaatgttg agttatttca tatacctctt taaatacatc atatactaca acattttcca
    16081 taagaagagt atctttattt tctacaaatg ataacagcaa tatatcatca atcaaccttt
    16141 ttaatctatc tgattcgctt tctattattt ctaagaatct atttctagta ttcacatcta
    16201 tattttcatt tgactttagt gtttcaacaa acccacttat agatgtaagt ggtgttttaa
    16261 gttcatgact tacatttgca acaaaatcac ttctcatatt ttcaagttta acttttttag
    16321 taatatcttc tatatttact atcgaaccta ttattacgtt gttagtattc tgaagataaa
    16381 ctgggtctac ttttatctta tatacgatat catctttagt agtaatatta gcagtttcat
    16441 tatctttaga accaataaat tgaagtattt gttttaatat ttcttcttct tttattacaa
    16501 cattaatatt atgaccttca actaattgat ttggttcact ctttatcatt tcttttgctt
    16561 catcatttat cagaagtata tttccattta catctatagc taaaattcca tgagatatac
    16621 tctttaatat agaccttagc tgtaaatgtt tatactgtac ttcttctaag gttttatcca
    16681 tattctttat catatgatta aaatttctag ctaattcgcc aagttctcct ttggctgata
    16741 tgttaagcct agcatgatat tcattattac ttactttttt tgagatgtat ataaactctt
    16801 ttaaatattt ccttaatgtt attgtgtatc ttacagctaa tataacaatg gctatggaca
    16861 acataacaat aaaaaaatat atactattta aatcttccac aagataacac tcctaaaata
    16921 taaccttttt aatctatttt gtatcctaca cctcttatag tctctatata tttttccgat
    16981 ttatcttcat cttctatttt ttttcttaaa tatcttatat gaacatctac tgttctagtt
    17041 tctccatagt attcataccc ccatatttta tccaaaagat aatttcttga aagtactttt
    17101 cctttatttt gaattaataa ttttaacaat tcaaattcct taagagttaa ctctatcttt
    17161 tcagagcctt tggtaacttc atgttttgat aaatctagtt ttaaattacc tgtagtaagt
    17221 atattgtttt cttcacctaa gctagtcaaa ttatatctcc ttaaaacaga actaatccta
    17281 gctaacaact cttttatact aaatggctta gttatatagt catcagcacc tatttcaagc
    17341 ccttcaacct tgtcattttc catattttta gcagtaagca ttataacagg aatatttttt
    17401 aagtccttat cacttcttat ttttctcaaa acttctattc cacttatatt tggtaacatc
    17461 caatctaaaa gtattaaatc tggtttaatt tccttagcct ttataaatcc gtcaaatcca
    17521 tcatatgaat aactaacttc gtaatttgac acctccaaat tgaattttaa tagctcaact
    17581 atatgcattt catcgtctat aacaagcact ttagtattca ttaaaaagct cctctctatt
    17641 ttatctgtag taccgttcct attcgttctg atgccttatt atgttttcta taagagtgga
    17701 atttatctgc tctacaacta gtgcataaat ttaaatttat tatattttct tttttaactc
    17761 cactacatct aagcatatac tcattaattt tccataaatc taaatagtaa cttccctctt
    17821 ttattatata gaacttttca tcattatttg taagaattgt gttaaatttt tctactaaat
    17881 cttcagacac ttcataacaa catggtccaa ttgatggtcc aattatacat actaaatctt
    17941 caggaactgt tttatagcat tcagacatta attttattgt tttatgacct atattactaa
    18001 aagtccctct ccaacctgca tgagccagtc ctatggcttt atttttagca tctataattg
    18061 atataggtac acagtctgct gtaaacagta aaagtggtac tttttccaag tttgttataa
    18121 gtgcatctcc atctcttctt ttccctatag tatctttatt gactatatta actatatctg
    18181 aatgtatttg agaattacta gttagatttt caaatgtcaa atcacattct ttacatactt
    18241 ttaacatatc ttctctattt tttgcatcta tattcttaca tgttataact agatttacag
    18301 aatttaattt ttcatcaagt tcttcacaat taccacttaa attaacatac tctttaatat
    18361 cttgtacatt ttttatattt atataatctt tcatcatttg tctccttttt ctatcagtat
    18421 acttttaagt tcatttacaa aagtatttat atctttaaat tgtctataca cagaagcaaa
    18481 tcttacatag gcaacctcat ctaactcttt caatttatcc attaccatct caccgatttt
    18541 tttggtttca atttcttcta taaaacattt atttatttca ttttcaatat gtacaacaat
    18601 actttcaatc tcgtcaatag aaacaggtct tttttcacat gcttttaaca atccatattt
    18661 tatcttttct ctatcaaaat attctctact attatccttt ttaatgacca ttaaaggagt
    18721 tgtttctatt ttctcatacg tagtaaatct ctttttacat gattcacact cacgtcttct
    18781 tctgatagat tttaaatctg tatgtctaga atctattacc tttgattctt tataattaca
    18841 ataaggacat tgcatctata ttcctccttt gtttaactat atctcaaact catctaagct
    18901 taattctgaa cctatattta ctattattgt atcacaacct attttaagta tatcattcca
    18961 aaatataact tcttcatccc ctcctcttga aaaaaaactt ctacttccat ctccaaaaat
    19021 ggtaaatgag actaccttac cttcgtgaat atccatgtct atgtctatta taaaccccat
    19081 cctttttcca ttttttacat ttataatttc tttttccatt atatcagaaa ctctaatcat
    19141 attgtactca ccctttcttt aaaaataaat atgtatcgaa gtaaaaaaaa atgacctata
    19201 taggtcattt aaattaaaat aatgtttaca gtccatggat tacttataca tattttctca
    19261 tattctttaa agcatttttt tcaattcttg atacttgtgc ttgagatata ccgatttcat
    19321 cagcaacttc tatctgagtt cttcctttgt aaaaccttaa atctaatacc aacttttctc
    19381 tactatttaa tttcttaata gcttctttaa gtgatatttc ttgcaaccaa ttttcatcag
    19441 tatctttttt atcttgaacc tgatccataa cgaatattgc atctccattg tcttgatata
    19501 ctgggtcaaa cagagatatt gggtcttgta tggcatcaag tgccattaca actgattcta
    19561 cttctaattc taattccttt gctatttctg atactgtagg ttctttagaa tttgttctaa
    19621 ttaatctttc tctaacttgt agtgccttat atgctatatc ttttaatgac ctacttactc
    19681 taattgggtt attgtctctt agataccttc taatttctcc tataatcata ggaacagcat
    19741 aagttgaaaa ccttacattt tggcttaaat caaagttatc tatagctttt ataagaccaa
    19801 tacaacctat ttgaaataaa tcgtctatat tttctcctct gttgttaaac ttttttatta
    19861 cactcaagac taatcttaaa ttccctctta caaactgttg tctggcttct tcatcaccat
    19921 tttttatttg taataaaagt tccttcattt gcttattttt aagaactgga agttctgatg
    19981 tattaacacc acatatttca accttattaa cttgcatata ttttcagtcc tctctcaaaa
    20041 gtattcatct attgaaatta tttacatttc caattttttt atacttttta agaagaatta
    20101 atttatcatt atacaaattt tttcatttct ttttgcaacc ttgagattat tttcttttca
    20161 agtctagata tgtaggactg tgatattcca agcattccag caacttcttt ttgtgttttt
    20221 tcaatacctt tatcaataag accaaatctt aattccataa tttgcttttc tctgtcactt
    20281 aatctgtcaa gtgccataac caacaaatct ctatctattt cttcttcaat tattttgtat
    20341 atttcatcat tttctgtacc caatacatca gataataaaa gttcgtttcc atctaaatct
    20401 atatttaaag gttcatcaaa tgatacttct gttttctttt tattattttt tcttaagtac
    20461 attaatattt cattttctat acatctcgaa gcataagttg ccaattttat atttttattc
    20521 agtttaaaag tattaactgc ttttatcaaa cctatagttc ctattgaaat caaatcttct
    20581 acatctatac ctgtattttc aaattttctc gatatgtaga ctactaatct aagatttctt
    20641 tctattaaaa ttgactttac actttcatcc gtttctagct tttgtaaaag ctccatttct
    20701 tcttctggtt tcaatggagg aggaagtata tttgcccctc ccatatagta tattcccttt
    20761 ggctttatta tttttaattt aatacctaac atagtaataa agcttattat tctttctttc
    20821 aatcgtaaca ttccttgtcc cccttaaatt ttcacctaaa atccaggcaa tatatttgga
    20881 ttcattattg ctccatattc tctatcatta aaatttgaaa taccaagtat tatatttcct
    20941 atcttttgct catctacttc tatatagtca gcttttaagc ctattattat gcttgtctta
    21001 ttacttccag catgtttata aggtattatt ctaactctac ctgaagtttt atcatctaaa
    21061 cttcctataa tttcttgtgc tttcttcata tctatatctt cataattaat atttaatagt
    21121 ttttctggaa taaatccttc taacacacta gacttaacta ttattacatc actttgactt
    21181 attgggtctt ttaacaaatt accactatct actaaagcct tacagcaaaa acttttttct
    21241 agtaagttta tatttatagt ctttgtaagc tccttaatgt acttaagcat tttcaaatct
    21301 ttataaatgt attttaataa ttcacaactc acataagtac atgcaattaa gaaagatatt
    21361 tttaagtgac ttattcctgt aaaataaatt ataaaataag tgcttccaga aataaaaatg
    21421 tttactagat aaaaaactaa cgctacatga acataatctt ttttgctctt acttctaaat
    21481 gaaattaaag ttattatact catgattatt attttaaatg gtacagtaaa tagtatattc
    21541 attgatggat acaaataagc aacagaatag gcacctccta aactagcccc caataactta
    21601 ttttttttag gattatatct tttagtaaga atagaagtac aacttataat aatataattt
    21661 attactaggt tttctataac atagtactca atatacacca tgtatccccc cttaaaacct
    21721 ttaaacatat tataagatag tatgtacaaa attttatgtc acttttgatg gtcgaatcca
    21781 aaacaaattc tatttaaaaa gaacaatgat atatgtctta tactttatgt cgtcaaaaga
    21841 tattttttta ataccaaaca aatagacttt tttgtagaat ccaatatatc taggtagaag
    21901 ttattcgctc tacttttggt atatttacaa aaaaagacta cataatttaa taatatatca
    21961 agtaaatctt ttacttatat aaaatcaaat tatatagtct gtaaattcaa atataaatta
    22021 ttgtgctagt aatccatttg catattttgc agattcattt agtgcttgtt ctggagttat
    22081 gtctccagct atcatcattt gaagttgttt ccataaatat tcatatatct ctactccatg
    22141 aggtccaact gtcaatggtc tatacatatg tttatctctt gtcaccattt cttcaaatct
    22201 tgggtctcct tggtattttt cacctttagc aactggagct ctaggtattt ctttgtggaa
    22261 agcttccatt gattctttgc ttagcatata ctcaagaagt ttaaatccaa gttctttatt
    22321 tttacatgct gacattaatg ttaaatcatc tactgaagca aaacttccaa ctgtcttatc
    22381 ttctaatgat gttacatatc cccagtttaa atccgggaat gatttatcaa aagtacttgt
    22441 agcagcagag gatagcatta tagtaaatgc agcttttcct ggtccaaata cactttcaat
    22501 catttcatta ctatcttttg ataaagcatc ttctggaata tactttttca tatcatttat
    22561 aaagtttgca gctttcaagc cttctgcatt attaaattta actgatttta aatcatcatt
    22621 atatatatct ccaccagctt gccataaata cggataccag ttccagttta agcttccaaa
    22681 tactttagaa ccccaacctt gtgcaagacc ccactggtcg atttttccat ctccatcagt
    22741 atcttttgtt gctttttcac agattctttt aaaatcatcc catgtttttg gaggtttttc
    22801 tcctaatttt tctagtatat ctttattgta gtacaatacg cctggattag cagcttctat
    22861 aggtaaacca taaataccac ccatcatttt tgcatcatct aagtatagat aattatctga
    22921 agttcctgat ttttctaaat aaggtgttaa atcttctacc gcacccattt ctataaattg
    22981 aggaaacatc tctgcataca tgtatcctat atctggacct tcacctgcac ttattgctgt
    23041 tgcatatttt tctgcgtagt tatcccatgg tactatctga aattcaattt cacagttatt
    23101 ttctttttca aatttttcaa atatcggctt ccatgcatct gcatcatggt ctgttaatgg
    23161 tgcagaccat acaactaatt tttctttacc ctctttgcct ttagaatctt ctttttttga
    23221 acatcctgca agaattgata ctgatagaat ccctaccata actaaagaaa gaaccttttt
    23281 tagtttaatc atgcttaatc ccctttcaga ctttaatttg tctttttcct caaatgttta
    23341 catttaattc agatttaata ttaacaaata agattactct tgtctaatca taaaaaacta
    23401 tactaactcc tataactata ttttttttct atacctatta gatttaattc atactaaaaa
    23461 cttacttttt aaattttatc aatatttaaa aatattttta agtctagcca caaactaact
    23521 atctaaattg aacaaaataa aaagaagtct attgacttct ttataagctt acatattatc
    23581 ttcttcttct taggaatgta ggtatctcca tgctctcatc atcatcttct tcaattatac
    23641 ttgccttttt aggaggctct attttttcct cagcaattac ttcttcttca atttctttta
    23701 cagcttgttt tactgttgta tttaatgaag aacgtatgct tgatttaggt ttagtatcta
    23761 aatctaaggc accattttgt aatccttcaa atcctgtcgc gattactgta atcattattt
    23821 catcacctaa atcttctttt atagaagctc caaatataac atttgcttct gggtcacagg
    23881 attccataac taaagttgat gcctcattaa cttcaaataa tcctaagtta ggaccacctg
    23941 taacattaag aagtactcct ttagctcctt gtattgatgt ttctagaagt ggagattgaa
    24001 tagcttgtct tgcagcatca attgctctag tttcaccact agcactacct atacccatat
    24061 gagcaagtcc cttgtctttc attatagtag taacatctgc aaagtccaag tttattaacc
    24121 cttcaactgc tataaggtct gaaattgatt gtataccttg ttttaataca tcatctgcaa
    24181 ctgcaaaagc atctaacatt gatgtatttt tttgtactat ctgtaaaagt ctatcatttg
    24241 gtatcgttat aagagtatct acttttgatt taagttctgc tattccacct tcagcgttct
    24301 tcattcttat cttaccttca aatgcaaaag gtttggtaac tacacctact gtaagtatcc
    24361 ccatttcttt tgccaagcct gcaaccactg gagcagctcc tgtaccagta ccaccgccca
    24421 ttccggctgt tacaaagacc atatcagctc cttgaagtaa ctttactatt tcatctttac
    24481 tctcctcagc agctcttttt cctacttctg gatttgctcc tgctccaagt cctctagtta
    24541 gtttttctcc tatttgaact ttatattcag cttttgatgt atataaagct tgtttatctg
    24601 tatttactga tataaattca acgcctttta gttgagcttc taccattcta ttgacagcat
    24661 tgtttccacc gccacctaca cctattactt taatttgtgc acattcttct aattctacgt
    24721 caaagtttag catcataaac ccccttctct catgattcaa agttatcgta tgtattaatt
    24781 atatcatttt cttcttttat tttgttaacc tttaagtaac atattccaca aaaacgtaaa
    24841 ttttcctttt taaatttctt acttatattg tcaaatatta ctctttaagt ataaaataat
    24901 ttacgtatat attaaatgct ctttaatttt tacgtttttt attcataata ttactaataa
    24961 tatatcgtct aattatagat aagttattaa acaatctcat accaaaaact aatacagcaa
    25021 catagtaaag agaaactcct aacttttctc ctatataagt caataatata gccaatatag
    25081 cattacctat gaaaccagta ataaaaatta cattatcata gttatcttca aaattggatt
    25141 ttatcgctcc aaaaatagaa tccattattg caagaatagc tactgacata tatatggaat
    25201 aatccataga atatgtaaat ggaatatagt aacctactac tacaccaaat gctaatcctc
    25261 ctaaaaccca taacataaat cttcacctct ttattttact ttgaaacaca actacattac
    25321 aatccttttt attaattttt tttacttttt ctttattttt ttgtattttt cgttttattt
    25381 tgcaaataaa ttcactttat ttccattatt ttcctgaact tttatattaa ttccatatat
    25441 atccttaaga gatttagcat atgattctgg agagtttata gctgcactca ataaatctat
    25501 atctcctata gcttttatta taaatggttg tccatatgta gtatcattta ctgtaatagt
    25561 agctcctgaa cattttatct tacttgttgc tagtactctt tctccattta tagaaattgc
    25621 tcttgctcca gcctttttta aatcatttaa aactctcaaa atatctatat catgtataat
    25681 caaatcgttt ggattctgtc cgtcttttaa ttctctctca ctatccctca tagtaatagt
    25741 tatgcctggc cctgtaatag ttgaataacc acttaattct ttaacttctt caagctcttt
    25801 ttgcataagt gtttggatag attcatttcc attctttttt gcttctttat attcattcaa
    25861 agtttttttg ttctttttaa gagtttcttt taagtttttt atttcttttt ttgtattttc
    25921 tacctcactt tcaatacttt cagtttgacc taatgttatg tatactctat ttgggtctag
    25981 tgattttata taagttgata tgaaaacacc taatatgaat gctcctaata taactatttt
    26041 ctttttcata ctattatttc ccctctgctt tgtttatatc actatgacca tttatttcta
    26101 tattgtcttc tttggtcact tttactttgg cattataata tgtttctatt tcccagacaa
    26161 taccatactt tatattaagt gccgaagcca atgtatcaga atcaccaatt gcttttatta
    26221 taattggttc ttttatgaca gtgtcattca tataaagctt gtcttctttt aaatacatat
    26281 atgtattaga aactattctc tggtcgttta tagatatggc acttgcttgt gctgaattta
    26341 acttatttat cattgacaat aataaatcat aattatatag tatactatca tttttatcag
    26401 agtttcctaa gtcttttgta gaatttatct caatttttat gcctgttcct tcaacatctg
    26461 tatatccagc taatgtttca tatttttcta tttctgactt aagtatgtct tgttctcctt
    26521 cattattttt gcctctataa gtctctatat ctttcttcac actattaact tctgattgta
    26581 gattctcttt ttcttttttt aaagatttaa gttcttctgt aagttgctct cctcttttaa
    26641 aagttgtcat ccctctattt tctatattaa taattttaag ttgtaaacta acaagaacac
    26701 ccaatacaat acttcctatg attatacttt tatttataaa tctttttttc aatatatcac
    26761 ttccttacaa ttgtctatcc ttctggacta tacactgcca actttccata gttaagattt
    26821 atagtccctc cattagtatt tttattttgc aggtcaatca gaattttact taccctagat
    26881 atattataat ctaagttatc atcattagaa agtaagattt ttatattact tcttgtaagc
    26941 atttctatat tgctttcttt ttttaagttt acataattta tttttctata aattccttct
    27001 tcttttaaat aatttaaagt tttaaataca ttctccttag ttttataatt tttaaatttt
    27061 atagatttat tatcatcaat gctataatct acatctacta tcaagtcttt tttactttca
    27121 ttagaacctc ttagttcttc caataaattt ccttttttat ctatataaca ataattatct
    27181 tcatctttta atactgcaaa tatttctttt tcttttaagc ttataattat cttattggga
    27241 agttttcttt ttatttccac attttctata tatggatttt ttataagttt atttttcata
    27301 tctttaaaat tgtatgcaaa aatattttca tttagattta catttagttc tttcattata
    27361 tttgatttgg tcaccctctt atttccaatt acatctattt tctttacatc aaataaatca
    27421 ctttttacaa ttatacatat acaaaaagat aacataagta taaaaactaa aactatcatg
    27481 acattattcg tatttaactt tcttcttttt ttcaaatata atcaacccct ttggttaata
    27541 aatacacctt tagagcttgt ttttcctaca aaaaaattat aatatttaaa gtcaaatttt
    27601 tgcattttat tacataacat ccaaataagg tattaaattt tataaataaa tcaatatttc
    27661 atcaa
//
LOCUS       DAKBNN010000026        27662 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000026 DAKBNN010000000
VERSION     DAKBNN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.026"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            131..1063
                     /locus_tag="OUB02_001044"
     CDS             131..1063
                     /locus_tag="OUB02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCT8884820.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGNFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            1168..1635
                     /locus_tag="OUB02_001045"
     CDS             1168..1635
                     /locus_tag="OUB02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HCT8884821.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(1879..2733)
                     /locus_tag="OUB02_001046"
     CDS             complement(1879..2733)
                     /locus_tag="OUB02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCT8884822.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            2899..4863
                     /locus_tag="OUB02_001047"
     CDS             2899..4863
                     /locus_tag="OUB02_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8884823.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            4863..5324
                     /locus_tag="OUB02_001048"
     CDS             4863..5324
                     /locus_tag="OUB02_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8884824.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            5327..5647
                     /locus_tag="OUB02_001049"
     CDS             5327..5647
                     /locus_tag="OUB02_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCT8884825.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            5663..6763
                     /locus_tag="OUB02_001050"
     CDS             5663..6763
                     /locus_tag="OUB02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCT8884826.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            6883..9483
                     /locus_tag="OUB02_001051"
     CDS             6883..9483
                     /locus_tag="OUB02_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCT8884827.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            9952..10287
                     /locus_tag="OUB02_001052"
     CDS             9952..10287
                     /locus_tag="OUB02_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884828.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            10424..11701
                     /locus_tag="OUB02_001053"
     CDS             10424..11701
                     /locus_tag="OUB02_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HCT8884829.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIRSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            11822..13210
                     /locus_tag="OUB02_001054"
     CDS             11822..13210
                     /locus_tag="OUB02_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT8884830.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            13581..14294
                     /locus_tag="OUB02_001055"
     CDS             13581..14294
                     /locus_tag="OUB02_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HCT8884831.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            14412..15281
                     /locus_tag="OUB02_001056"
     CDS             14412..15281
                     /locus_tag="OUB02_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCT8884832.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(15436..16581)
                     /locus_tag="OUB02_001057"
     CDS             complement(15436..16581)
                     /locus_tag="OUB02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCT8884833.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(16915..17691)
                     /locus_tag="OUB02_001058"
     CDS             complement(16915..17691)
                     /locus_tag="OUB02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCT8884834.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            18273..19247
                     /locus_tag="OUB02_001059"
     CDS             18273..19247
                     /locus_tag="OUB02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCT8884835.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            19345..19650
                     /locus_tag="OUB02_001060"
     CDS             19345..19650
                     /locus_tag="OUB02_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCT8884836.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            19806..20966
                     /locus_tag="OUB02_001061"
     CDS             19806..20966
                     /locus_tag="OUB02_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCT8884837.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            21016..21918
                     /locus_tag="OUB02_001062"
     CDS             21016..21918
                     /locus_tag="OUB02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCT8884838.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            21998..22891
                     /locus_tag="OUB02_001063"
     CDS             21998..22891
                     /locus_tag="OUB02_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCT8884839.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKVEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      22944..23125
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            23353..24483
                     /locus_tag="OUB02_001064"
     CDS             23353..24483
                     /locus_tag="OUB02_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8884840.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            24555..25580
                     /locus_tag="OUB02_001065"
     CDS             24555..25580
                     /locus_tag="OUB02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCT8884841.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            25583..26779
                     /locus_tag="OUB02_001066"
     CDS             25583..26779
                     /locus_tag="OUB02_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884842.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(26907..27632)
                     /locus_tag="OUB02_001067"
     CDS             complement(26907..27632)
                     /locus_tag="OUB02_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCT8884843.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT        10730 a         3073 c         4748 g         9111 t
ORIGIN      
        1 gatacttttg ataataaaat taaacttaat agatattttc tatataagaa agatggtcat
       61 aatatgtcaa tttgaccatc tttttgatat aattaaaaca caaataattt agtaacaaag
      121 gaggcagaac ttgaatttat taaaaaaata tcctatacct attgcaagtt tgattttatc
      181 aatttttact ttaggtaact tattacaatc ttatagtgaa tcattacgta atgtaattgg
      241 atttatagga tttattttgt atgcaattta tatagtcaaa ataatattac ttagaaaggg
      301 tgttaaagag caattagaga accctttaat atcaagtaca tttccaacta taacaatggc
      361 aacaatgata tttgctacat atataaaacc attatcttta gaattaagta ttatgatttg
      421 gagcatagga attgtgggtc atcttgtgtt aatagtctta tttagtaaga agttcttagt
      481 taaattctca ataaaaacgg tatttccatc ttggtatata gtatatgttg ggatagttgc
      541 agctagtgtt acggcaccag catttaatca acttttgatt ggcaaaattg ctttttggat
      601 aggatttgta tcttatatag ttcttatacc agtaatttta aagagagtat ggataatcaa
      661 ggaaatgcct gaaccatctt taccaagtat agctatatta gctgcaccag ggtcactatt
      721 attgtctgga tatattaatt ctttttcaga taaaaataat gtagtattat acttgttatt
      781 catattatca attgtatttt atattatagt tttgggatat ttaccaaaat tattaagatt
      841 gcctttttat ccgagttttg ctgcatttac attccctatg gcaataagtg caatgggtat
      901 gaaactaatg aatggttatt taactaaatc aggtaatttt ataagttgga tttcatatgc
      961 ttctaagtta gaagagataa ttgctacagt gattatttta tatgttttag ttttgtttat
     1021 tatatatctt agtaaatctg aaactacatc taaagctaaa taagaattat taaatatgtg
     1081 tatttaatag gaatattgat ataaatatat gaagataaga taaaccatag aaacttaatt
     1141 tagcttaatt ttattggagg aaaacaaatg aaaacaaaag aagaatattt aaaagaagtt
     1201 agaaacatat cagaagaata ctttagaaaa ggagagtttt tttgctctga agcagttctc
     1261 cagacaataa atgatgcttt aggccaacct ttaagtccag agataactaa gttagcatca
     1321 ggatttccga taggacttgg aaaggcacaa tgtctttgtg gagcaatatc tgggggagag
     1381 atggctttag gtatagtata tggaagagtt catggtgaag ctatgaatcc aaagatgttt
     1441 gagcatgcta aaggtttgca tgattatatt aaaaaagagt atggtgcaac atgttgtaga
     1501 gctattacaa agaaatggga tggtgataac tttatgtctc ctgaaagaaa agagcactgt
     1561 attaaaataa caggtcaagt tacagagtgg gttgcagata agcttataga agatgaaaaa
     1621 cttaatatca aatagtgtaa tattagttgg tgaatttata aagttaaatt tacttatagt
     1681 ataagatact tgatattctc gtattgatag aaaaatttat agcatatgta tttaatgaaa
     1741 tatacttata tttagagtgt ggtttaattt cataaataca tatgctatta ttttaagtat
     1801 ataagcaaat ttatttaaat aaatagaaac agttaattta tatgtacaaa actatatatg
     1861 attagtgtaa ataaatacct attttataat actcaacata aagacaccag acatgattag
     1921 tattatacca ataaaaatag caagccattc ttttttgttc ttcttttctt taaaaaatat
     1981 aatccctcct aaagtggcta ctatcacaca actctgagag atagaaaaac ttattgtaac
     2041 tcccaatgta ttagtcgtta tgaacatacc taaatttgct attgaccaca atataccagt
     2101 aattaaattg aataaaacat tttccctata tacaatctct ttagaaaaat acttgacaat
     2161 aatagatgaa gttatcaaca taccaacaga ttgaggtaat ataattctgt atccttcaat
     2221 taaaaatatt tgattagtta caacatataa tgataaaaat agagatgata ataatacaac
     2281 tccaacacat tttaacatat ttttatctgt agatttttta gtttgataat ttgtgagaag
     2341 tataccaagt attatacata atattgataa agaaccaatc agtaacattt tagaactggt
     2401 ccattcgtta aacaagatta cagctaataa tgtcgtaaaa gttaattgtg aaccatttga
     2461 aattggcatt actttagata cactagcaat ttgaattgct ttaaactgca ttaattgtcc
     2521 caaactccag aatatgccag aaatcatact tatacaaaat gaaaaaaatg taatttcagg
     2581 atgtataacc agagttatta tagtggaaaa tataaacgca cttatggatg tacctaaaag
     2641 ctgttcatga ggagaacttt tctttagatt tgcaactatg ggcatcaatc cccatcccat
     2701 tacaggaagt aaagcaataa ttattgtcag cattaaacta tttcctttca tattaaattt
     2761 agacagcttt taatttatca tctaaaatta aagtgttcaa tacataaaaa ttaccgtatt
     2821 taatcaggaa atattagtag ttttttagaa aaacattttc cattaaaata aagacaaagg
     2881 gaaggagtaa aattgaaaat gttaactgat atgattcaaa atcgtgaaat taaaataata
     2941 aagtttttat taaaagaagg tcatacaact gtaaaagcaa tagcagatga tttagatgtt
     3001 tcagaaaaaa cagtttcaaa atctcttaaa gaagtagaag cagtgcttaa aaagataggt
     3061 ctttctttaa taagaaaacc taagataggt gtttatattg atggagattt aaaaaaaata
     3121 cttccttacc tagataataa aggaagtcaa attcctacaa ccagagagga gagagttatc
     3181 tatatatatt cagtactttt aaagagtaat gattatatta ctatacaaca actttctgat
     3241 gaactatata taagtcgtgg aactattgaa agggatttag cagaagttga aaaactgcta
     3301 aaaaacgaag ggatttccat atataagaaa cctagtaagg gaataaagtt aaatataagt
     3361 gaaagagata aaagacaact tactgctaaa tttatacata aattttggag tagaaactgg
     3421 tatattaagc aagagcaaga aagcttttat caagcatttg aaaagataca agcagatgta
     3481 aatggtattt tttcggaaga tgatttatca ataattattg atattcttag gaatttcagc
     3541 agagaaagaa aatttgagtt ctctgattat gaatttcagt cattagttat tcatttggca
     3601 atagctgtag agagaattag aaaaggcgaa tatatagaag aaagtttgta tactggtaat
     3661 atagaaaata atcaaatgtc aaataccaat tatttagcaa aacaattaga agaaaaattt
     3721 tcaatattat taccaaaatc tgaaattgga tatataaatt tacatttagt agcgtctgac
     3781 caacatagag atactaatga actaaaaaat acttcaacta atccttgtga gacaagtaca
     3841 aataatatat caaatcttcg ggaaattata aaaaatacat tgtatgagac agcttatgac
     3901 aaggatttaa ttgatggatt aacttcacat gtacattcat caataaatag actgaaatat
     3961 ggacttagta ttaaaaatcc atatgtaaat acaatcaaac aaaacttttc tttatcattt
     4021 gagcaagctt tagatttaaa gaaagtaatt gaatttatat atgatattag gataaatgat
     4081 gatgaatgtg cttatatagc attacatttt gaagctttta gagaaagaat gaaagaactt
     4141 ccacaaaata ttagagtaat tctagtatgc agtacaggat taggttcatc taggttattg
     4201 tctgcaagaa taaaaaaata ctttccaatg ataaagattg aaaaagtact ttccgtacaa
     4261 gaattagtat ccagtaaaat tgatgcagat ttagtaataa gtactattta tttagagtta
     4321 gaaacaattc caaccatagt agttagtcct attttaaatg aaagtgatat aagaaccttg
     4381 gaaagaaata tacaaaatgt ttcaagttgt acttctaaga agtataaatc tttcagaaat
     4441 ttaatctttg aagaaaatat attgttgaat attgaagctt caaattatga agatgcaatt
     4501 aatcatataa cagataacct aataaaaaaa ggttttgcaa aagaaggtat agctaaaagt
     4561 gcaattgaga gagaaaattt atcttacact tctttccaat caatagctac acctcatgct
     4621 aatcctagtt atattaaaaa atctaatata tctgttgcaa cgttaaaaaa tcctattaca
     4681 tggggaaatg aaaaggtgga agttattttc tttatttcat tagaaaatga caaaaaccta
     4741 gagcttgatg aaatatatga agttttttat gactttattg acaataaaaa aaatataaat
     4801 gacatattaa gtgcaacttc cacaaaggaa gtgtacgatt tgctagtgga ggagagtata
     4861 tagtgaaaat atcagatgta ttgaaaaaag agcaaattgt ttttaatctt gatacaaata
     4921 caaaggaaga aacaataaaa aaattaagta gtatatttgt tgaaaatggg attgtggaca
     4981 atgaagaaga atatgttaag tctgttttgg aaagagaaga acattcaact acaggtattg
     5041 gtaatggtat tgctattcct catggaaaaa gtaatgctgt caaaaaatct tctataatat
     5101 ttgcaaagac taagcataag attgaatggg atgctttaga tgaaaaacca gtagatagta
     5161 ttttcttatt ggctataagc aatattgata aggatagcaa tcatcttgta ctattatcta
     5221 aattagcaac taaacttatg gatgatgaca atgtagatgc actaaaatta gccaattcag
     5281 aacaagaaat aatcaatata tttagtgaag aaggagagat gtagaaatga aaagaaaaat
     5341 tatagcagtt acagcatgtg caacaggagt tgcacataca tatatggcag ctcaagcgtt
     5401 aaagaaagct tcaaaaaaga tgggtcacct tataaaagtt gaaacacaag gagctacagg
     5461 aatcgataat gagttaacta caaaggatgc acaaattgca gaagtagtaa tatttgcagt
     5521 agatactaaa gtcagaaatg ctgaacgttt tgagggaaaa gaaatactag aagtaccagt
     5581 taatgcacct ataaaggatg cagagaaagt aataaatgat gcactaaagt taattgacac
     5641 taaataaatg gagggaaata taatgttgaa aaagatagtc tctgagttaa aaaagcatgt
     5701 cttaactgga atctcatata tgattccatt agtaattgca ggtgctatga taatggcgat
     5761 atctcgtgta ggtggttctt tctataatat accagatata tgggatgcta aatatgcaga
     5821 aagtgctagt agtattgtta gattattaca tgatttagat gggtttggag gtactgcatt
     5881 aggattaatg tttcctgtaa ttgctgcatt tataggattt tctatagctg ataaattagc
     5941 tattgtacca ggtcttgttg gaggtatgat tgctaaagat ataggtgcag gatttttagg
     6001 agctttggca gttggtctta tagcaggata tacatgtttg tttattaaaa atcatgttaa
     6061 attaccaaaa tcagcagctt caatagtacc aatatttata gtaccagtat ttgggacatt
     6121 aatcactgtt gtattaatca attatgtgat aggtatacct tttgccacat taaatactgg
     6181 attagaaaat tggttaaatg gattatctgg tacaaatcag atattaatgg cagctattat
     6241 aggggctatg atggcagttg acttaggtgg accagttaat aaagcagctt taacaacatc
     6301 acttgcatta ttaacaagtg gaatttatgc gcctaacact gctgcaatgg taggaatagt
     6361 tataccacca ctaggattgg gacttgctac tatactagca aaacaaaaat ataataaaca
     6421 gttacgtgaa gctggaaaat catctcttat aatgggttta attggagtaa gtgaaggagc
     6481 aattcctttt gcagtagaat ctccattaaa agtaatacct tcatgtgtaa ttggaacagc
     6541 aattgcttca gctatggcag ttggtttagg ttcagtaaat gcaactccaa ttagtggatt
     6601 ttatggatgg tttacagtag agaattggcc aatgtatgtg ttatcaatag cagttggaac
     6661 agtaattgta gctggattag ttatagtatt gcgtggaaat ccagtgattg aggatgatga
     6721 atttgaagat gatgattttg atgaagcaga gtgggaatct taaaatttaa taactattta
     6781 agacgtgtta taagctatat tcaatatgaa tcttatacta aataaagtgt taaacattca
     6841 gtttagactt aaatataaaa gataaagaac agaggagaat aaatgataaa agcacatatt
     6901 gttaatcata cacactggga tagagaatgg tattttactt ctggagatgc attagtactt
     6961 agtgaacaac tatttacaga tgtaattgat gaattagaac gtaatccaga tgtaagtttt
     7021 gtacttgatg gacaactttc tattttggat gattatgtac aattacacaa agaaaaaata
     7081 gaaatcatta aaaagttaat aaaagaagat agactacata ttggaccatg gtttactcaa
     7141 actgatgcat tctttgctag aggagagtct attttaagaa acttaatgat tggaattttt
     7201 gaaagtaaaa agtatggtaa gtatatgaaa attgggtatt tgccagatac atttggattt
     7261 aatgcacaga tgcctatact attaaagcat gtaggtcttg ataatatatt cttttggaga
     7321 ggtatacatc ttggtaaaca ggttcaatca ccatatttta agtggaaaag cttaaatgga
     7381 gaaagttata tatatgcagt aaatatgcca catggatatg gaactggtat gcttttagag
     7441 ccttcattaa agtatgttaa tggacgctta gacccagcta ttgattttat taaatcatat
     7501 actgatgtag atgaggtgtt aataccttct gggaatgacc agttaaatat aataggtgat
     7561 ttcaaaaata aaattaaagc gattaatgat atcggaaagt attcatatat aacgagttca
     7621 tatcaagagt ttttaaaata tataaagtct atagaatcac tagaaagcta tagaggtgaa
     7681 tttagagaac ctgtattagc aagaatacat aaatcaattg gttctagtag aatggatata
     7741 aaattagcat gtgataaact agaaaacaag ctgattaaaa gaatagagcc attattagta
     7801 attgctaaaa agagtaaaat tgaaataagc aatcagttac ttataaatac atggaaaaaa
     7861 cttttggaag gacaagcaca cgatagttta gctggatgtg taactgatac tgtaacagat
     7921 gatattcttc accgtataag agaagcaaat gaaatatgtg acagtattga aaatactatt
     7981 gtaaaaaaga tttcagaagg gcttaaatta tctaaaaatg atattttagt attcaataca
     8041 gaagctaagc gttttaatgg atataaagag atacaagttg taagtgatag taaaaatatt
     8101 tattttaaag ataatttaga tgctactata attgaagaaa cttatgtaaa accgagagaa
     8161 aatgtattag aagaaacacc tgctggtaat atatttattg aagaaccagg atattatata
     8221 ttaaaagtaa gaataaatat acagcttcct gcacttggtt ataaagttgt tagttttgaa
     8281 ttaagtgata aagaaatggt gtcacttgat aaatctaatg acacttttat atctaatgat
     8341 aattataaga taatatatga agatggaagc ttaaatttac aacttaaaga tggaacttac
     8401 atatcaaact ttttaacatt aaaagatagt ggtaatgcag gtgataccta tgacttttct
     8461 ccactaaaaa atgatgagga tataaaactt tcgtttgata aagtgtatac agaaaaagct
     8521 ttaggatatg aaaaaatggt ggttaaaggt actactttac taccacttac attagaagat
     8581 agaaaaaaca aatatcttaa tgggaaatta aatgtaaaag taagtattgt cttatctaaa
     8641 gaaaacccat tgatggatgt aaaactttgt gttgataaca cgatatataa tcatagacta
     8701 cgtgttcata taaaaactga tataaaagat aataaaaaca tagcatcatt gccatttgga
     8761 tatataacaa gagaaaatgg agttttagac aactgggaag atatttacag tgaaatgcct
     8821 atagatttag aacctttgga aagtaatatt acgcttacaa accaaaatag aagttgtact
     8881 gtatttacta gaggaataaa agagtatcaa catattgaag atgaaattgc attgacctta
     8941 cttgctacta cagatgaact aggaaagcct gatttattat atagaccagg acgtgcttct
     9001 ggagatacaa ctaaaaaagg tcatataaga attaaaacag ataaagctca aatgcttaag
     9061 gaattagaat tttcatttgc aatatatatg gattctaaat catttgatga attggaaatt
     9121 gcgaatttaa cccacaatta tctaagagaa tgtgtaaatt atcaattaca ggattacaat
     9181 ttctttttat atcgtataga taataagatt caaaaatcaa ttgtcaataa aactgtaagt
     9241 agagaattag aaataatatc tttaccagaa aactatctta tatcagcatg ttatccttct
     9301 tattacagta aagataaatt tataataaga gtggaaaatc cgagtagtaa tagaatgata
     9361 ttggatgagc aaatatttaa aaatagaaat gggaaaattg taaatgcaat agaagatgta
     9421 gaagaagaac aggtatatga aatagcacct tttgacgtta tttcaatact attggattta
     9481 taagtgtaaa agtaagttta ttgtttaaag tctgacagat attaatctgc cagacttttt
     9541 aatttgtata aatttgggta aatattaatg aatggtttta gtatttaagt atgaatttag
     9601 tatttaatat agaatttatt gaagtagaat aattagttta acaggataag cagtttaaat
     9661 aaagaattat tatattagag tgattttata tataatttaa aataataaaa ttttaataaa
     9721 atgagtgaaa gattgtatat taatagagtt gatgtagtta caaaaaagtt actaattgtt
     9781 atgtatttac aaaactgata tattaattac ataaaatcta aaatagtttg taataggagt
     9841 atatataata aaaattttaa ttaaataaag ctaggtatat ttataatatt aataaatata
     9901 ttgctataaa agtttgttta ataaattaaa taaagaaaag aggagaataa aatgtacaac
     9961 atagatgata tgatatataa ttgtccagta gaagctttat caaatatttt aggtaaaaaa
    10021 tgggtggcta taattatatg gaaaatacaa gaagataaaa aacgatttgg agagctacaa
    10081 agagctatac cagactgtag taagaaaatg ttagttcaac aactagattt tttaattgaa
    10141 caggggatta tagtcaacca aaaaaagttg gttaataatg ttgtagagtc aacatatttt
    10201 ttaagtgaat caggattacg attgcttcct gtcatggaaa aaatgattat gtggagcaat
    10261 aaaaatttaa attgtgataa atactaaaat ttactctgta agtaatgaaa atttaattaa
    10321 gctatgagta gaaaaatgta ttaaaattac ataatttttt agttagattt taatttagaa
    10381 ataggattgt aatattggaa ataaaataag gaggaatttt aatttgcaaa atgtaaattt
    10441 atttttagta tttatagaag gtatagtatc attcttttca ccatgtatac tgccaatttt
    10501 acctatttat ttgagtatat tgtcaaatag tagtgtagaa aatttaaaag aaggaaagac
    10561 gagttttata ggaagttctt tatttaaaaa tactatattt tttgctcttg gaatctcaac
    10621 aacattcttt atattgggtt catcagtaaa agtattgagt atgtttttta atgaaaataa
    10681 agatttaata atgtttattg gtgggattat aattattatc atgggattat tttacatggg
    10741 aataattaaa tcttcaatat tgaatagaga aaaaagattt aatgttaagt ttaaagaaat
    10801 gaaagcaata acagctttta tattaggttt tacgtttagt tttggatgga caccttgtat
    10861 tggtccaata ttggcatctg tattagtcat ggtttcaagt tcaagtaatc atcttagtgc
    10921 caatctttta atagcagtat atactattgg ttttattttg ccattcataa ttacagctat
    10981 gttttacagt aaactattta aaactataga taagataaga tctaacatgg aaattattaa
    11041 aaaaataggt gggattatac ttatagtatc aggaatatta atgatggtaa atggatttgg
    11101 aagtataagc aaacacttta atacgtcaca aaatagtaaa attgaaagca agcaagaaga
    11161 aaataagcga gaaaattcta ctgataaaga agaaaattct gatggtaatg atagtcaaaa
    11221 agactctaat aatgacaata atgataaagg gtctaatgat gaagatagaa taaagtctat
    11281 tgattttact ttaacagacc agtatggtaa aacacataaa ttaagtgatt atgaaggcaa
    11341 agttgtattc ttaaatttct gggctacatg gtgtcctcca tgcaaagaag aaatgcctta
    11401 tatagaacaa ctgtataaag actataataa aaataatgat gatgtagtaa ttttaggagt
    11461 agcttcacct aatttaggta gagaaggttc aagggaacat gtagttaact tcttaaaaga
    11521 ccaaggttat acattccctg ttgtacttga tgaagatgga gcattagcat atcaatatgg
    11581 aattaatgct tttccaacta catttataat tgacaaggaa ggatatgtaa ctcagtatat
    11641 tccaggagct atggataagg caactatggc atcatttatt gaaaatcaga gaaataaata
    11701 aaagtattta agtataagtt tataaatcaa tttaaggtta caaaaaagtg cctaattgta
    11761 ccacctaaat aatcttgtta tagtaaaagt ataaacaata caagaaaggg ttgaatttta
    11821 tatgaaaaaa acatttgatg ctataataat aggatttgga aaaggtggaa aaacattagc
    11881 aggggatttg gcaaacaggg gattaaaagt agctttaata gaaaagtcaa acaagatgta
    11941 tggtggaaca tgtgtaaatg tggcatgtat accaacaaaa agcttagaaa attcagcaaa
    12001 cagcgtaaaa acaaagaata taaattcttg ggatgaagta caagctgaat atgagaaagc
    12061 tatagataaa aaagaaactt taattacaaa actcagagaa gctaactata ataaattaaa
    12121 ttcaaatgag aatgtaacta tattcacagg aatgggaact ttcattgatg aaaaaacagt
    12181 tcaagtcaaa actgaaaatg aaatttatga gttagtagca gataatatat ttattaatac
    12241 tggttcaaga ccatttatac ctaatattaa gggaattgaa aataagaata tagtatatga
    12301 tagtgagtct cttatgaatc taagaacttt acctaaaaaa atgacaatta taggagctgg
    12361 atttataggt ttagaatttg ctggtattta tagttcattt ggagcagaag ttacaatatt
    12421 aaactctaat aatggcattt taccaaatga agatgttgag gattcagaag aaataataaa
    12481 attacttgca aagagaaatg taaaaattgt taataatgca aatataaaag agattaaaga
    12541 agtttctgaa ttagcaatag tagaatatga agtagatggt aaatctaaag aattaacaag
    12601 taatatgata cttgtagcta caggaagaaa agcaaacact gaagggctag ggctagaaaa
    12661 tgcaggtata gaattaaatg aaagaggatt tattaaagtc tcagaaactc ttaagactaa
    12721 taaagagcat atatgggcta taggagatat aaatggaggt ccacaattta cttatatttc
    12781 attggatgat tatcgcatag ttattaatca gttatttgga gataagacta gaacaactaa
    12841 tgatagaaag aatatcccaa attctatatt tataagtcca gcattttcaa gagttggtct
    12901 taatgttaag caagctaaag aaaaaggata tgaagtatta gtggcaaaga tgccagtaga
    12961 agcaattcca agagctaaac agattggcaa ggcagatggg tttataaaaa tagtgataga
    13021 taaaaaatcc aataagatac ttggagctag tatgatatgt gaaaattcaa gtgagattat
    13081 ccatcttatt caattagctg tagatttaga agtagagtat acatatttaa gagaccgtgt
    13141 ttatgcacat ccaactatga cagaagctct taatgatatt ctatctccta atatgataaa
    13201 agaagtataa cataagttta atatataaac ctaaaattat cattataagt agattttaga
    13261 aagaggctat ctcaaaaaat agagataagt ctctttttta ctgtttaaat gataaaaata
    13321 tatttgattt tataaaagaa gattatctta tgaactaaaa agtttatttt aaaacactac
    13381 ctatttatgt tataaactta taaatataat atagaatcta ttattctgta ttattaagtg
    13441 gggttaagcg ttataattaa ttataaatat tgaataatct aaatggataa gataatggta
    13501 ttaaaaatta tcttaaaagg tgaaataatc tgtatttaat gtagtacagt tatatcttta
    13561 aagcaaggtg gtgaaatgat ataaataaga tatctaaaag agggtttgat gatagtgata
    13621 aaagatggtt ttcaagtaaa gaattaacaa gacttataaa ggctaaagaa gaaatagaat
    13681 ggttattaaa tagggattac aaaatagacc cagtcgttac ttttgttggt gatagatatc
    13741 agttttctat aagacaaaga gatgcattaa aaagggcagt ctgtactgat gaaaaaaata
    13801 ttataagaca atcaaagagg ctttctctag ataaaatcaa agaagatacc gtaaatatag
    13861 atggctttaa tttgataata tccatagagg tagcattatc tggtggtact ctagtaatag
    13921 gaagtgatgg caatataaga gatttagcag gtcttagggg aacctataag attatagata
    13981 aaacagaaga agctttgaat ctgataggta aattttttaa taaatacaat gcacagaatc
    14041 ttaaatttta tttagatgct ccagtttcta attcaggaaa tttaaagtat aggatattag
    14101 agcatgccaa aacttgggga attgaaactg aagtagaatt agtaaaaaat gcagatgttg
    14161 tattggaaaa attagataga gtagttagta gtgatgcagt catagttgac aagtgtataa
    14221 gttattttaa tgtggctaga ggtataattg aagagtatat taaagaatgt aatatcataa
    14281 atttaaataa atagataaat ataggtgaga atatataagt atatgtagca atattattag
    14341 taatacaagt aaagtttgaa aataggagaa cgaatacata tcgaatttca taaaatgttg
    14401 taaaaagaca tatgagagga tggatatgtg tgaaattaac taaactaaat attaaaaaat
    14461 atagagcacc tatatataaa tatgctttag caaatgtaaa tttgaggtct gctaaatcta
    14521 ccaattcaag tatcataaca gtaatacctc agggagctaa aatggaagta ttggatgaag
    14581 aagatgactg gattaaggtg atgtacaatt ctcaagaagg atatgtgtat aaggatttgg
    14641 tatcagtaag cgaatatgct tggagtaact taaacttaag agaagataaa tctacaactt
    14701 ccaatataat tacagtaatt cctgaaaaat ctagggttga agtccttcaa gtagatggag
    14761 attggagtaa ggttgtatat gatgataaaa taggatatgt ttttaattat tttttatcaa
    14821 ttgatgggaa taaaccaaat gaattagatt ataagtattt ttatactgac atgattaaat
    14881 ttgttaacga aaataatatt aaaagtacta gcgattatct aattgtaaca gaccttagaa
    14941 ataaatatac ttatattttt aagaaagata atggaggctg gggacaactt tacaagtggc
    15001 aatgtactat aggtaagcct gagactccaa ctataacagg tatattttat ataagtggaa
    15061 gaaaaccttc ttttggaacg gatgagtatt cagttaaata tgctactaga attaaaggtg
    15121 gatattacta tcattctgtg ctatatgatt caacaggaag ttatataata gatggcagac
    15181 ttggtgaagc tcttagtcat ggatgtataa gattaagtac ggaaaatgca aagtggatat
    15241 atgataatat accagataca acaacagtta taattcatta agaaaaattt tgagtttttt
    15301 attgggataa tgattcttaa attaatagga ttatatatta acttctcata aagagtacta
    15361 tttaaaaagt gtaataagag aatgctatta acaaaacaaa gatattttga ggcactctct
    15421 tataaatata cataattaag atattttcat aaacaattct ataatcttat ctttatcaaa
    15481 gaaaacagga ttagcaccag cacaagcatc tttcatagag ttttctgcaa gcaattcaaa
    15541 attaggatta tcaacaccta catcttttaa agattttggg attccaacct cttcagaaag
    15601 agcttttatc ttatctatta caaagtcaac acactcttta tctgatttat tatttacatc
    15661 cattccaata acttcagcta ttggtcggaa tttggcaggg gctgatttag cattttcctc
    15721 ctcaactatt ggaagtagca tggcattaca tactccatga ggtaaatcat ataaacctcc
    15781 taattgatga gccatagcat gtacatttcc aagacctgca ttactaaagg caattccatt
    15841 taaaaaacaa gcataacaca tttgttctct agcttctatg tcattaccat ttttaacagc
    15901 tcttggaagg tatttaaata tctgctttat agcatataag gcagttgaat ctgtaacatc
    15961 ataagcacca ttagctacta cagcttctat agcatgagtt agtgcatcca taccagttgc
    16021 tgctgttaaa ttagatggtt tgctaatcat taattctggg tcatttactg tcattgtagc
    16081 tagtgaattg gtatcaacca ttatcatctt tacatgtctg tcttcatcag ttataacata
    16141 gtttattgta acttcagcag atgtaccagc agtagtagtt atagctatga taggaagaca
    16201 ttttttagaa gttttgttga taccttcata gtctctaata tctcctccat tagtggctaa
    16261 tatagagata gctttaccac agtcttgtgg agaaccacct cctatagtta taacgaagtc
    16321 gcaattttct gatttcaata ttttaagtcc tttattcaca ttagttacag ttggattttg
    16381 tttaacttca tcataaacaa caaagtctac atctatctga gataaaatat ctgttacttt
    16441 ttttacagta ccatttgaaa ctagaaattt atcactaaca ataaatgctt ttttagttcc
    16501 taatgattta attgaatctt tagcttcatt aagacatcct ttcccaagta aatttattgg
    16561 tggtacataa taaatgctca taactaaaat ctccttttat tttcattgat ttttatgtaa
    16621 aaatatatat ctaacaattt tcactaattt actcataata tatcgtatct tagattgaga
    16681 tagggttgct ggttcttata taagaactta attcgtaatt acgattatga ttagaattta
    16741 acatagtatt tttatcatgt caataaaaat ataaaaaata ttataaaaaa aaacaaagtt
    16801 actgtagtta aatcagcaac tttgttttta tagtataaat caaataaata cattagttta
    16861 aaacaaaatt tttaattatt atgttttgat atcttccata tacagaatat taatctaatt
    16921 tattacaatg tcttcattac gttcttccat cattgattga atatttttct gagcatttaa
    16981 gagtaatttt tttatcagtt ctattttttc gtcattagct ctaaataaag gcagtgttac
    17041 actaacagca gctcttatac atccattttt tcttattggt acggcaagac attttaggtg
    17101 ttccattgat tcctcacact ctgaagctat ttgtgttctt tgaacttcaa gaagctggtc
    17161 gtataaaaca tcaaaatctg taattgattt ttctgtaaaa ccttttagac catctggata
    17221 taagcttatt agtttttcct tgtcaaaatc acataaaaga gcttttccaa gacctgtaca
    17281 attagcaggt attttttttc caacataaga aataagtcta attggttctg gagaatctac
    17341 ttttccaata taaagaactt cattattgtc aagtatacca agctgacaag tttcagaaca
    17401 ggaattaact atattagtca tttcgtcaat tatatagtta aaaatagttc ttttttcaaa
    17461 aaatgaagac cctaatgtgt aagaacgtat ccctatagaa tatttggatg tgtttttatc
    17521 aatagtaatg tattttctag cattaagtgt atgaactata ggaaagatgc tactttttgg
    17581 agcattgata acatctgcta tttctgtaag agtatatcca ttttcggatt ctgctaatgc
    17641 ttctaaaata tctaatatcc tagctgttgg gcgatgatta tctgaagtca taaatttccc
    17701 cctcttttta ttaatttaaa gtattagaat atgttgattt tagcacacta tacgatattt
    17761 tacaatgtag aaaataaata tgtaaattta tatataaata agctcttgat ttagatacag
    17821 atagatattt ttttaggctg tacattaagg aataactaat ttccaaaata tacctaaaga
    17881 aagtaagtga tatttagtta tataatttaa gtaaatatat gcaatagtga tttttatatg
    17941 taaatatcaa aatattatga acttatattt tatctatttt ttacactgta taaaaacttt
    18001 atagttttga atactatata attttaaata cgctgtattt ttgcatataa ggaagtcatt
    18061 tgataatata caaggtatta tcagaaagtt tgaaaaaatt ttgacaaaaa tttatttttt
    18121 ttattgactc ataaatagga aaatgctatc attaattcaa agattcgtaa ttaaaaatct
    18181 agttcttaaa tacgaaccga aacggccgtt agaattaaag ctaaaaactt aataagagta
    18241 attaaatatc atgttttagg aggattaact ttatgataat ggattttatt aaaaaaattc
    18301 ctgcaggtat gatgatagtg ccaatgttta tagcagcttt cattaatacc ttttgcccag
    18361 atattgtaca aataggttcg tttacgacag cagtattttc aagtgttggg tctgcaacta
    18421 taattggggt tcaactggta tgcttgggaa ctcagcttca atttagagaa atgccaaaag
    18481 ttttaaagag aggtggtata cttcttggtt ctaaatttgc tataggtgct ttgataggta
    18541 tacttatagg aaagctattt ggaatggatg gtgtccttgg tctgacaaca ttggctgtta
    18601 taagtgcagt tacaaatagt aatggtagta tatatctttc attgatgact tcatatggag
    18661 atgaaacaga ttgtgcaact atgagtttac ttactttaaa tgatggacca ttttttacat
    18721 taatagctct tgggacttca ggtcttgcaa atgtaccatt gttgtcatta ttggctgcaa
    18781 tagtgccaat acttgttggt atgataatag gaaattttga caaaaaaatg aaagagtttt
    18841 tagagccagg gtgtaattta ctcgtacctt ttgtagggtt tgcacttggt agtagcataa
    18901 atcttgcaag tatactaaaa ggaggtttat caggagtatt acttggatta atatctgtat
    18961 ttgtaggagg tatatttata gtattctgtg ataaatttat aggaaaacgt ccaggatatg
    19021 caggatgggc tgtagctaca actgctggaa atgcaattgc tgtacctgct gctgttgcat
    19081 tagttgaccc atcatggcaa ccatatgttg caacagctac aacacaagtg gcagccgcag
    19141 tagttgtcac tgcaatatta gtacctctta taactagttg gtgggctaag aagtatggat
    19201 gtcctaagtt cccaaaacaa aatgaggatg gtacaataac agcttaattt atattacagt
    19261 gagttgttgg taaaataata aattctttaa ttagatttta ggatattaat aaaattatat
    19321 tatttataaa ggagagcaag aataatgata aatgcagtga taattgatga aaaagataat
    19381 gttgcagtag ctattgaagc aatagctaaa ggtagtgaaa ttagttttaa attaaaagac
    19441 aaaacagtaa aaacgattac agcactagat gatataacta tttatcataa attggctata
    19501 aaagatatgg ccaaaggtga taaagttact aaatatggtg aacatatagg agaagcaaat
    19561 caggaaataa aaagtggtca acatgtacat attcataatg ttagaagtgt tagagaggat
    19621 ttggataaac aagttatgac taatatataa aataatattt atagagttat aaaagttgta
    19681 ttcattgctt ttagtgtcaa atagttttta aataggtttt tagctgtttg attagtgagt
    19741 gtaataaaaa ttataatata taaaaattat aaaaagtata tagactattt taggaggaaa
    19801 tcagtatgaa attttatgga tataaaagac cagatggaag agttggtata agaaaccaca
    19861 tattaatctt gcctacgagt gtgtgtgcat cagatacaac tagaataata gcatcacaag
    19921 ttcaaggggc agttaccttt aataatcaga atggatgttc acaagtacat tcagaccaac
    19981 aattaactat ggatgttatg gctggttatg cagcaaatcc aaatgtatat ggtgtaatag
    20041 ttgtatcttt gggatgcgaa aattgccaaa atgatttggt agttgatgca ataaaagaga
    20101 gaacaaataa gccaataaaa actcttgtaa tacaagaaga acatggaact ttaaaaacta
    20161 tagaaaaagc tgttcgttat gctagagaaa tggcacaaga agcttctttg cttagaaaag
    20221 aagaatttcc aatatctgaa cttatactag gaactgaatg tggtgggtct gacccaacaa
    20281 gtggattagc agcaaatcca ttaataggtg aattaagtga taaacttgtc gatttaggag
    20341 caacatcaat tttaagtgaa actactgaat ttataggtgc agagcatata ctagctagga
    20401 gagcagtaaa tgaagaagta aaagaaaaaa tattacatat agtacataga tatgaaaatt
    20461 cattaaaatt ggttggagaa gaagttagag aaggtaatcc atctcctgga aatatagctg
    20521 gtggtttaac aagtttagaa gaaaaatcac ttggttgtat acataaaggt ggtcatagac
    20581 aaataagtga agtatatgat tatgccaaac aaattgataa gaaaggcctt gttataatgg
    20641 atacccctgg aaatgatgct tcatctgttg ctggtatggt agcaggagga gcacaaatag
    20701 tggtatttag tacaggaaga ggaacaccat caggaaatcc aatagcacca gtaataaaaa
    20761 taactggaaa taaaataact tttgcaaata tggaagataa tatggatttt gatgctagtc
    20821 cagttatata tggtccacaa acaatggaag aattaacaga tgatttgtta aatatggttg
    20881 tagatgtagc aaatggaaaa caatcaaaag cagagtccct tggatatact gaaatggcaa
    20941 tagctagagt atgcaactat gtataatctt taagtaagaa aataaataca tagaatttta
    21001 ataaagggtg agattatgaa gtttgtaagt tttactgaag gagaaaatga atgtaaaaga
    21061 actggagttt tttctaaaga tgaattattt attatagatg ttaactcttt aaatctcagt
    21121 agaaatttta aggatttaaa tgaattaatt caaaatgtat ctagtgaaga tattattaag
    21181 ttgaaaacta ttataaatga aagtacattc tataattatg atgtttttaa aagagagaga
    21241 tgtactgtac atgttcctat agaaaaacca atacatgata tcttatgtgt gggagtgaac
    21301 tataaagacc atcttgaaga aactcaaact cactttgatg atagttttga agaaccacaa
    21361 aaaacggttt attttacaaa acgtgtgaca aaggcaatag gtccagaaga tgagatagat
    21421 ggtcattttg agataaataa tcaactagac tatgaagttg aacttggagt agtaataggt
    21481 aaaacaggtg ttaatattag aaaagaagag gtagaagatt atatctttgg ttacactgtt
    21541 ataaatgata tttctgctag aaagttgcaa aaagaacatg ttcaatggtt tagaggaaaa
    21601 ggattagata catttacctc tttaggtcca tgtattttac ataaaagtgc agttcctttt
    21661 cctataaaac ttaaagtatg tagtagggta aatggtgaag aaagacaaag ttctaataca
    21721 ggattattca tatcagacgt ttcagacata gtttcagaac tttcaaaagg tatgacctta
    21781 gaggctggag acattatagc tactggaaca ccttctggag tagggatggg attttcacct
    21841 cctcgatata tgagtagtgg agatgtagtt gaatgtgaga ttgagagtat tggaatttta
    21901 aaaaattaca ttaagtaact gttttaatat aattaatatt aaagctaaat ctgatgtatt
    21961 aatgaacata ttaaaataaa ttttaggagt ttaaattatg aaagcagaat ttttgacacc
    22021 agttgttaca atatttgatg aagaaggaaa tttagataaa gaaggtaata aaaaagtgta
    22081 tgaccatctt atagatggag gtgtagatgg actagtcata atgggaagta ctggagagtt
    22141 ctttaatatg tctatggata tgcaaaaaga acttatagat ttagtaacta gtcatgtaaa
    22201 taagagagta aaagtgttta taggtacaag tcgtatgtgt ataaatgaat ctatagaact
    22261 ttcaaactat gcacataata gtggagcaga tggtgttatg ataataagcc cttattattt
    22321 ctctcttgat gatgatagta ttgagttatt ttatagtgaa atagctaaga atacagaggc
    22381 tgaaatatat ttgtacaatt atccagatag aactggttat gatttaagtc cagaacttac
    22441 attaagactt gcaagaaaac ataaaaacat agttggatat aaggatacag taactttgat
    22501 gggacatacc agagatttaa taaatactat gaaagtagag ttcccagaat ttaaggttta
    22561 tagcggttat gatgaaaact ttatacataa tctaatgtct gggggagcag gttgtatagg
    22621 gggattatca aatttaattc cagaagtttg ctccaaatgg gctgaagctt acaataatag
    22681 agatatggag aaggttatag aaattcaaaa atatattaat aaagcaatgg atttttactt
    22741 aataaccaat ccatttatac cagcaatgaa aaaagctatg aatatcagag ggttacaaat
    22801 aagtgattat atgactgctc catttgtaaa accaaatgaa aaacaagtat gtgaaattaa
    22861 aaaattgatg aatgaattaa atatatgtta aatcaacctt gcaaagaggt caacaataaa
    22921 ttacaattat cttaataaaa attaaattaa aataatacta gggtactata ttagtttaat
    22981 agggaaagtg atgaaaattc actacagccc ccgctactgt atacggatac gaaatcaaaa
    23041 tccactgaaa tttataaaaa ttttgggaag ggtgatggag gaaaagccgt gagtcaggag
    23101 acctgcccag tattatataa gttcaattta gggtggagat tgtaatttat aataaaatga
    23161 agtatcaatt cgtttttgta aagttgatat atgaacttct ccttattaaa ttaggagaag
    23221 ttttttattt tattaaaagg ttatacttat taaagatgac tttaggataa ctttaataag
    23281 tatactttaa aaagcatata ttattaaagt tttttagcca ctcataaaaa ataagatatt
    23341 ggagtgaaag agatgaaatt taaatcaaat gttttaaagc agtttttttt aattgctatg
    23401 gtaattttta tggtaggagg tcttataggg tgtagtaaaa gtgacaaaga gaataaacaa
    23461 gctagtactg gtaagaaatt agaattaaaa tatgctagtg gatttagtgt tgaagattta
    23521 gaagatggaa ttaaaaaagt aactgatgga gataatcgag aactgatttt aatcccaaag
    23581 aagattaaaa ttcctgaaaa atataaaaat gctaatatag ttagaacgcc tgtggacaat
    23641 gtattaatag catctacaac acaggcttgt tctctgagaa caatagatga actagatagt
    23701 ataaaagcag taacaacaga agagcagtac tggactatta aagaaattaa agatttaatg
    23761 aaagatggaa aaatacatta cgttggttca aatttagcac cagattatga aaaaatagta
    23821 gacttaaatc ctgacttaac tatagttagt agtggtttat cagaggctga tacaaaattt
    23881 attgacaaat taaaagaatt agggcttaat tatgttgtag acaatgagta taaagagcaa
    23941 aatccatttg gaagaatgga gtggacaagt ttaatagctg cattttatga taaagaagat
    24001 atggctacta gtgaactaaa taaaactgta caaaaggtag aagaagtaag taaaaaaata
    24061 aaaaacaaag ctaaacctaa agttgtatgg gcatctgtat atgaaggaag tgcttatata
    24121 gctcctaaag attcatatgt ggataatatg ataaaaatgg ctggtggaat aaatgcatgt
    24181 gcttcaatag attcaaatga agggcgagta agtattgaac aattgcatga agttgcaaaa
    24241 gatgcagata tatttgtata ctcatcaagt tcagattatg caccaaattt agattctata
    24301 aaatctagtg cacctgtatt ggctgatttg gatgttgtaa aaaacggaaa cctatgggtg
    24361 tttgctccag attattatca atcagtagat aagacagacg aattaatagt agatttagtt
    24421 gagatgttcc acccaggtac tacaggtgaa aaaatcaaac attatataaa ttatgataaa
    24481 tagtacagag aggacacaaa aagataggtt ttatagattt tagttaatat agctttgcta
    24541 agaggagtat acaaatggat tttatcaata aaatgagaat aaggaatacg acaatattta
    24601 tagtattaat tataagtatg atacttactt ttttagtgtg tgtagcattt ggctctgtaa
    24661 aaattgaatt taaagaagta atagatgttg tattaagagg tatggataat acagaacatt
    24721 ataaaataat atacaatata agaatcccta gagtacttgc aaccatggta ggtggagcat
    24781 gcctagcagt atcaggtatt ttattacaga tattttttaa aaacccaata gttgaaccgt
    24841 atatattagg agtatcttcg ggttctactt tatcagtagc tctcatgatt ttgggaggat
    24901 acacattggg atttgagagt gtaaattcaa tgagtatatt tgtagcagct tttataggag
    24961 ctttaattgt aatgtctata gtcattttat ttgcaaataa ggtaaagaat ataacaactc
    25021 ttttaataat aggaattatg accggatatg tatgtagtgg tataacaaat gtgttacaat
    25081 catttgcaaa tagtgataaa ttaaaagaat ttactatgtg gtctatggga agtttttctg
    25141 gatttacatg ggaaaaggta gaaatactta taatagttgg tatattaggt cttttgatga
    25201 ctgtattcat aataaaacct ttaaatgctt ttttactagg agaagagtat gcaaaaagta
    25261 ttggcataaa tataagattt tttagagttt tgatagtatt tatatctagt atattagcag
    25321 caatagtatc agcatttgca ggacctatag ggtttattgg acttgcagtg ccacaaattt
    25381 caaaaatgat atttaaaaca tcagataata gagttttgat accagtagta atatttttag
    25441 gcagtatagt tacagctctt tgtgatttaa tatcaagagt aattttatca ccagtagaat
    25501 taccaataag tacgataaca tctttattag gagctccaat tgtagtaatg ttattggtga
    25561 aaaggaagga tagggcttag acatgttgaa aactaataat ttaagtgtag gatacaataa
    25621 caaagttgtt ataagtaata taaatgtaga agtaaaaaat ggagaaatac tatgtctttt
    25681 gggaagtaat ggagctggaa aaactaccct attaaggtcc ttatctaaac ttatatcacc
    25741 aataaaaggt gaaatatatt tgaatggtgt aaacataaat tgtatatcaa gaaaagcatt
    25801 atcaaaaaaa atggctttag tacttacaaa tagattatta ggtgatttaa tgactgtaca
    25861 agatatagtc aatataggaa ggtatccata tacaggattt tttgggagtc tatctaagaa
    25921 agatttaata atggtagatg aagcattgga gtctgtagat gccttacatt taaagaaaag
    25981 atattttgat gaactaagtg atggagaaaa acaaaaggtg ttggttgcaa gggctcttgt
    26041 tcaagaacca gaaataatta tattagatga gcctacaact cacttggata taaaacatag
    26101 attggaactc ataaacatac tgaaaaaatt aagtaaagaa aagtcaatat cagtaatact
    26161 ttcattgcat gaaatagaca tagcattaaa aagttgtgat aaagttgctc ttataaaaaa
    26221 taataaagtc atagcttatg gtcagcctga agatgtagta gatgaggata ttataaattc
    26281 gctttatgaa ttggatgata aaaactttaa tagtctttta ggttcagtag aaattagtaa
    26341 taaatctaaa aatgaagttt ttataattgg tggtgggggt aaagcaacac caatttatag
    26401 agcttttact aaaaaaggta taggtctgta ttctggaata atacatgaaa atgatattga
    26461 ttatgaaatt ggtagaacta tggggataaa gatgtttaca gaaaatccat ttgagcctat
    26521 aagtgatgag agtttcgatt tggctattag aaatttaaat gattccaaga ttataataga
    26581 tacaggattt agtgtaggag agacaaataa gaggaatata gatattataa aagaggcttt
    26641 aaaattggat aaaaaagttt attcttttag aaatagagat gagagtaaga agtattatga
    26701 cagtttggat agtaagatag aacatataga taaagtttct caaataatca atagtgcaga
    26761 tataaataat ttattataat ttatgaaata aaatgtactt atgtgctata gaaaaaagag
    26821 tatagattat aatttttttg aaattacaat ctatactctt ttgttatttt gtttatgtat
    26881 ttatatattt ataaaacttt agcattttat agataaccaa tagcttcctc tctagttaaa
    26941 aaaaagtgaa tccctgtagt cgattctttc catctatttt cattaaaatc ttcagctaca
    27001 atcgtttctc ctactctata tataaaattt tcatcagcat atgcacgagc acttttaaaa
    27061 gattctttac catctatact agtaattgac agtacttttg ccttatcaca tctacaagcg
    27121 tttgatgtag cagacactct ttttgcgtcg ctgggtatta ataattgtac cattctaaaa
    27181 ttaaaacatt ttttatagcc tataaatgct cctgtttcag gacaatacaa tttaaaaaaa
    27241 cgggtacttt catctgatat tactccattt aaagtagcat gttctaaatt tgaattttct
    27301 aaattagctc ctctaatatc tgagttagat aagtcacaaa atctaaaact tgcaccactt
    27361 aagtctgcat tttttaatat agaatttttt aatgaacaat ccgaaagata agaattatta
    27421 aaaatagagt tttctaaatc tgctttttct aaatttaaat tgataaggtc agaccacgaa
    27481 aagtcaatat tacttaagca gtatcctctt aagtccatat tttctaatct taattctttt
    27541 aaatctgcct tttctccagc attattgttt ttaagccata aataatgttt ctcaagtgtt
    27601 ttttctaatt cttgtttaga aatagttttc attaaaatca ccttcataaa tttattttta
    27661 ta
//
LOCUS       DAKBNN010000027        27594 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000027 DAKBNN010000000
VERSION     DAKBNN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.027"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            198..1763
                     /locus_tag="OUB02_001068"
     CDS             198..1763
                     /locus_tag="OUB02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8884844.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            1777..3108
                     /locus_tag="OUB02_001069"
     CDS             1777..3108
                     /locus_tag="OUB02_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HCT8884845.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKVQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            3122..3610
                     /locus_tag="OUB02_001070"
     CDS             3122..3610
                     /locus_tag="OUB02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884846.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            3624..4091
                     /locus_tag="OUB02_001071"
     CDS             3624..4091
                     /locus_tag="OUB02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8884847.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            4094..5248
                     /locus_tag="OUB02_001072"
     CDS             4094..5248
                     /locus_tag="OUB02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8884848.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSVLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            5564..6316
                     /locus_tag="OUB02_001073"
     CDS             5564..6316
                     /locus_tag="OUB02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884849.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            6317..8224
                     /locus_tag="OUB02_001074"
     CDS             6317..8224
                     /locus_tag="OUB02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8884850.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AVLKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            8376..9056
                     /locus_tag="OUB02_001075"
     CDS             8376..9056
                     /locus_tag="OUB02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884851.1"
                     /translation="MYRILLVEDDIDLSKEIALAFEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            9050..10069
                     /locus_tag="OUB02_001076"
     CDS             9050..10069
                     /locus_tag="OUB02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8884852.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNIEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            10500..11660
                     /locus_tag="OUB02_001077"
     CDS             10500..11660
                     /locus_tag="OUB02_001077"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCT8884853.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREIFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            11662..12060
                     /locus_tag="OUB02_001078"
     CDS             11662..12060
                     /locus_tag="OUB02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884854.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKEMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            12322..12630
                     /locus_tag="OUB02_001079"
     CDS             12322..12630
                     /locus_tag="OUB02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8884855.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            12719..14065
                     /locus_tag="OUB02_001080"
     CDS             12719..14065
                     /locus_tag="OUB02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8884856.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            14298..15395
                     /locus_tag="OUB02_001081"
     CDS             14298..15395
                     /locus_tag="OUB02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCT8884857.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLKH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            15494..16408
                     /gene="murQ"
                     /locus_tag="OUB02_001082"
     CDS             15494..16408
                     /gene="murQ"
                     /locus_tag="OUB02_001082"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCT8884858.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            16518..17438
                     /locus_tag="OUB02_001083"
     CDS             16518..17438
                     /locus_tag="OUB02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HCT8884859.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLDHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            17455..18306
                     /locus_tag="OUB02_001084"
     CDS             17455..18306
                     /locus_tag="OUB02_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8884860.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            18918..19979
                     /locus_tag="OUB02_001085"
     CDS             18918..19979
                     /locus_tag="OUB02_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HCT8884861.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKAPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(20095..21069)
                     /locus_tag="OUB02_001086"
     CDS             complement(20095..21069)
                     /locus_tag="OUB02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884862.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            21525..22406
                     /locus_tag="OUB02_001087"
     CDS             21525..22406
                     /locus_tag="OUB02_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HCT8884863.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            22788..23591
                     /locus_tag="OUB02_001088"
     CDS             22788..23591
                     /locus_tag="OUB02_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884864.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            23603..24733
                     /locus_tag="OUB02_001089"
     CDS             23603..24733
                     /locus_tag="OUB02_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8884865.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            24737..26146
                     /locus_tag="OUB02_001090"
     CDS             24737..26146
                     /locus_tag="OUB02_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884866.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            26357..26866
                     /locus_tag="OUB02_001091"
     CDS             26357..26866
                     /locus_tag="OUB02_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HCT8884867.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT        10838 a         2829 c         4804 g         9123 t
ORIGIN      
        1 ttacagaagg gattgattgc aaattgtagt cattcttttt tttatgagaa tttgaacata
       61 aaaatgtgca aaaacttatt tgactgtgaa taagctccga atatacccta aagattgtat
      121 atatttatta acctgttaaa ataatagata tctaaggcca aagataaact aaaaatatga
      181 ggaggggagt aatttatatg atgaagaaaa tacaacgttt tggaggggca atgtttacac
      241 caacactatt atttgctttt gctgggataa tggttggatt ctcaattgta tttcaaaatc
      301 agtcaattat gggttcactt gctacaccag aaaatatttg gtaccaattt tggggtgtaa
      361 tatctagtgg agcttggatg gtatttaatc aattaccatt attatttgct atttctttac
      421 caatagcact tgcaaaaaag caacaagcaa gagcttgtat ggaggcttta gcaacgtatt
      481 tgacttttaa ttattttgta ggttctatgt taagtttttg gggaaaaagc tttggagttg
      541 atttcgcagc tgaaataagt gctggaagtg gacttgtaag cattgctggt attaaaacat
      601 tagatactgg tatggttggt gctttgttaa tatcaggtat tgtcatttat attcataata
      661 gattttatga taaagaactg ccagatttta ttggattatt tagaggctca tcacttgtag
      721 ttgcaatttg tttctttgtt atgataccag ttgcattatt aacttgtttt atttggcctc
      781 atattcaaaa tgttattaga catttgcaaa cgttttttat aaatagtgga aatataggag
      841 tttggtgtta tgcattctta caaaagattt taataccaac tggattacat cattttgtat
      901 atgcaccaat ttgttatgat agtgttgttg ttccaggtgg tacatcagta tattgggcta
      961 ctcatattca ggactttcaa acttcagcta agactctaaa ggaaatgtat ccaatcggat
     1021 tttcattatc cggattatcg aaggtgtttg gttcattagg tgtatttggt gctttctatg
     1081 taactgcaaa gccagaaaaa aagaaaaaag tattaggttt aatgattcct gctactctta
     1141 ctgcagtatt aactggaatt actgagccgt tagaatttac atttttattt gtagcaccac
     1201 ttttattcct agtacatgca tttttagatg catgcttgca aacgatttcg tttgccctag
     1261 gagttgttgg tgattttggt ggaggaatta ttaactgggt tgtattaaat tggcttcctc
     1321 ttggaatgta tcactggaag gtatatgttg ttcaagttgt tgtagggatt atattttcat
     1381 ttatctggtt ctttgtattt actttcttaa ttaagaagtt tgatatgaaa actcctggta
     1441 gagaggaaga tagtgaagaa acaaaactat atactaaaaa tgaatattta gaaactaaag
     1501 atgaaaaagg aaataaattg agtaaagcct ctcaacaagc atctgaatac ataaaattag
     1561 ttggtggagc agaaaatgtt gtagatgtta caaactgtgc tacgagattg agattaacct
     1621 tgaaagatga ctcaattatt tctaaagaag aagattttaa ggcagtaggt gcacatggtt
     1681 tagtgcataa tggaaaagct attcagatta ttattgggct ttcagtacca agtgtaagag
     1741 aagaatttga aaatttatta taaaggagaa ggaaagatgg aaagaaaaag acagaggata
     1801 ctaattgctg gtgggggtag tacttatact gctggaattg taacaatgtt aattgaaagt
     1861 gttgctaaat ttcctattga atcaattaaa ttatatgaca ataatgatga gagacaaaga
     1921 aaagttgctg aagcatgtgc aataattgtt agagagaaaa atccagaaat taaatttagt
     1981 tatacaacaa atccagaaga agcatttact gatattgatt ttgtaatggc acaaattcgt
     2041 gttggtttat atgctttacg tgaacaagat gaaaaaattc cactaaaata tggggttgtt
     2101 ggtcaagaaa cttgtggggc aggtggaatt gcctatggat taagaacaat aggtccaata
     2161 atcgaaatga ttgattatat ggaaaaatat tctccaaatg cttggatgct taattattca
     2221 aatccagcag ctatagttgc agaagcatgt cgtgtattaa gaccaaattc aagaattatt
     2281 aatatttgtg atatgccagt atgtttagag gaaatatttg ctagagtttt aggtttaaat
     2341 tctcgtaaag actttgatgt tagatattat ggattgaatc atttcgggtg gtggacaagt
     2401 attaaggaca aagaaggaaa tgatttaatg cctaaagtac aagaatattg tgctaaaaaa
     2461 ggatatgagg aatttacacc tcaaggtcaa cacaaagaat ctagttggtt agaaactatg
     2521 agagcagcaa aggatttatt ggaaatagaa ccgactacat tacctaatac ttatttaaaa
     2581 tattatttaa tggctgatga aactgttgaa catgctaatc caaattatac aagagcaaat
     2641 gaaattatgg atagaagaga aaaggataca ttcgaagaat gtgaaagaat tataaaaaat
     2701 ggcacagcaa gagatacatg gtttgatgca agtgaacatt ctcaatttat tgttgaatta
     2761 gcttgtgcat tagcatttaa tactcaggaa agatttttac tgattgttcc taataatgga
     2821 gctattgaaa attttgcaga tgatgcaatg gttgaaattc cttgccttgt tggaaaagat
     2881 atggttgaac ctatgtctat aggtaaaatt ccaactttcc aaaaaggttt aatggaacaa
     2941 caagttgctt ctgaaaaatt agctgtagaa gcatggatag aaaaaagtta tcaaaaatta
     3001 tggcagtcgt ttactatgag taaaactgtt ccaagtgcaa aagtagcaaa agaaatctta
     3061 gatgaaatga ttgtggctaa taaggatttc tggccaactt ttaaataagg agaacaaaaa
     3121 catgataaca aataaaataa aaaatatcac agcagtatta aaagatgaaa atatatttct
     3181 ttcagtggtt atggtttgtt ttctaatatt acctgtttta tatatagtgt atttagaatt
     3241 gacatcatta tttacaaata tttcattttc acaactttta aacaatgatg ttaatgcaag
     3301 tttaaattta atctctaact ttagtgcagt atacagtgca tatgttattt acaatataaa
     3361 aaagaataaa gagataaaag aaaacatgat tgctttctat ttagtcttat tagggcagat
     3421 attccaaatt tacacaataa ctattgttat catgtttacc tatatttata aatttgttgg
     3481 aattaaacaa atcataagca gtattaaaga actacaatgg ggaaagagct ataaggctct
     3541 tttttcctca attgcagtat gcttaatatc gttatttgta atgataatta gaataaagtt
     3601 aattatgtag aggagtgtct ataatgttct ttaaaagaaa aaaagaagca attacaatta
     3661 attcctacat tgatggaaaa ataattaatc ttggagaaat tcctgataaa gtattttcaa
     3721 ctaaggtaat gggagatgga tttgctatga tagcaaagga caatattctt aaatcaccga
     3781 ctaaaggagt aataagtttt attgctcaaa ctaaccatgc tataggaatt aaattgcaaa
     3841 atgatgttca aattcttatt catattggat tggatagtgc aacaacaaaa aaagaaaact
     3901 ttaaattaga agttaaacaa ggtcaagaag ttaatatggg ggaaaagtta gtagaaattg
     3961 ataataagtt acttgaaaat actttatata ttcctttagt tattgtagag aatcctaata
     4021 cggtaaaata tcatctttca aacttaaatc aggaaggaaa tagtggtatt aaagttatca
     4081 attttgattg attatgatta ataaagcgat taaatttaaa tttatcatat tctatttttt
     4141 ctcatatatt atttttgcct ttgcttcaac aaaattcaca cctttcttat caaaattagg
     4201 ttactcagcg tttgaaagag gaattatatt atcgagctat gcaataacca atatattgtt
     4261 tcaattatta tttggagtat tagctgataa gtatcaaaca atgaaaaaaa ttgttttaat
     4321 tagtttatca gtttatggaa ttgcatcagt tattatgttt tctactgaat ctgcaacttt
     4381 tataatatac ttattacttg ttgctttatc aggaggatta ttaaatactt gctgtagttt
     4441 atatgataca tggataatag gttgtggaga taatattaat aattatttgt catttattaa
     4501 gacttttggg tcgattggat gggctattgg aagtatggta gcatcttact tgattcttag
     4561 attctcatat tttggattat ctgtagcgat tggtttaatt gtagtattac ttattataaa
     4621 tatactatta ttacctgata ttgaaaagat taataagaaa ataaatgtaa ctaataaaga
     4681 tattattgaa ttattaaaag ataaaaaata tatcttacta gtatgtattt tattcttatt
     4741 atactcaatg gtggtagcaa ataactgtac tgtaatagat aaaatgatag ctttaaatgc
     4801 tacaaattct cagatatctt taaagtggtc tttacagtca gtgttagaga taccaacata
     4861 tctggctggt tcttatttat taaaaaaata tagcagttta aacttattaa aattaagtgc
     4921 tattatgata acagttcagt ttctactatt tgctttaaca aatgattcaa atatcattat
     4981 tatactgtgt gtttttcaag tgttttctac tcctttaatt ttaattactt caaaaagatt
     5041 aatatttgaa gtttctccta aaaaacttag aagtagtagc caattaatag ctttaagtat
     5101 atttatggga ggttcatctc tgattattcc aactgttgct ggttttttaa gcattaatat
     5161 tgggtataat tatacattaa tggtattaag tctttttgga tgcttagctt attgcttgat
     5221 agtcttattg aattatcttc aggaataatt gttttaatat atagtagtaa tatcacttgt
     5281 ttatagtatc tgtatttgcc acaatcaatg tgtattttgg ttttttatag agaagaataa
     5341 ttgtatagat tagaggcttc ttaggagctt cttttctttg tttatacaaa gaagtaaatc
     5401 atgcttttat tttaagatat ttccattata attataatag tcttccatat gttacaaaag
     5461 tgtaatgcta acagttaaaa tgtaatattt acttaaggat ttatttatat aaaaccttta
     5521 aaataaatat atattaaaaa attagcaact ggaggctatt attatgaaag aaatattaaa
     5581 aataaaaaat atatcaaaag attatggaat aaaaggattt aaaacaaatg tattaaaaaa
     5641 tatatctctt acagtcaatg aaggtgattt tatatcaata atgggtccat ctggtgcagg
     5701 aaagacaaca ttgctaaatt taatgtctac acttgacaaa caaacttcag gagaaataat
     5761 actagatgga attaatatat caaaagtaaa aaataatgaa ctatcaaaat taagaagaga
     5821 aaaaataggg tttatatttc aagactataa tctattagac aatatgaaac taatggacaa
     5881 tatagcactt cctctagcat taggaaaaaa gaaaagtaag gagatagaag ctaaggtatt
     5941 ttctatagct aaaaaatttg gattagaaaa tcatttagat aaatatccat atcagttatc
     6001 aggaggacaa aaacaaagag gcgcagcagc taggtcatta attacaaatc caacagtcat
     6061 atttgctgat gagccaactg gtgctttaga ctctaaatca gcatatgaac ttttagaatc
     6121 attagaaaaa ataaacagag aaaataacgc tactattata atgattacac atgaccctct
     6181 aacagcaagt tattcaaatg aagtatatat gataaatgat ggaaatataa agtgtaaatt
     6241 aaacaaagga aatagtagaa aagaatttta tggaaaaatt atggatatgc tagcctctat
     6301 gggaggtgag atgtaagtga gtatacttgg aattgcttat aataatttta agagtaacat
     6361 aagaacttat ctagcatttt ttatatctat ggttttttca gtagttgtgc taactaattt
     6421 tgaattatta agatatggtg atgctataaa tgtgttacaa gatgaaaata aaaaatttac
     6481 actatcagtg ttagtagctg taataatgat actatcagta tttttatttt tctttatatg
     6541 gtatgcaaca aatatatttt ttaagaatag gtctaaagaa attggaatat tgtcttttat
     6601 gggattagat ttatatacta taggaaaaat atattttgta gaaaatatgc ttataggaat
     6661 aagttcttgt ataacaggaa taattatagg gataataaca tctagatttt ttcaagtagt
     6721 gataataaag ttatctggat ttaatataaa ggtatctaat ggacttagta tagaagctat
     6781 actatatgct actttaatct ttatttctat atttataata atggctgtaa aaggttttat
     6841 aacaatatgt agaagtagtg taataaatct aatcaatata tctaaaaaac aagaaaaaat
     6901 accaaaggta ggaataggta tatatatatt agctatagta tcaattatag taataggata
     6961 tggctactat ctttctacca atataagaac tggagatata actagtgtac tacctgttat
     7021 aggtataata atagtaggta cttatggatt attcaaatca gttatgcctg ttatatttga
     7081 tattataatc aaaaataaaa aaatattatt taatggaaat aatataattg caataaacaa
     7141 tataaactac cgattaaata aaaactataa aacttatgct ataatagcta taattataac
     7201 tacaacaata tcaactcttg gtgcttcagt tgcgatgaag cgtatacata agaatgccca
     7261 agaacaaaga aatgtgtaca ctattagcat agtttcaatg gataaagatg aagtgaataa
     7321 aaaacaaata gagaatataa taaaagatac taataagata aagtactcag taaatccaga
     7381 actaacaatt ttaaagaaaa cagatataaa agaagcttat gatacaacag atactataat
     7441 gaaatatagt gatttttgta atactcttaa agtgaatggc aatgataaag aattaaaaga
     7501 gtatggtgaa agattagtta gtggaaataa tgtaatagag ttaaaaagcc ctcaaacgat
     7561 agcatcaata gctacaaaag aagccaagat aaaacttgat ggtcagttat ataatatttc
     7621 aaggggagaa gtaaaagttc cagtactagg gacaggactt aatcaaagta ttatagtggt
     7681 aaatgatgat acatatttta aactaagaga taagggtcaa caagtgtatt tttatggtgc
     7741 aaaggtattt caagaagaaa atagtcaagt aatgttcaac aatattgaaa agaatttaag
     7801 tttaaaaaat gtgtatattc aaaatggata taaagctcaa ggtgagagtc aatggatgaa
     7861 gtttgcatat gtagtgcttg tgtttttatt cttagtcttt gcaattgtag ctggaagtat
     7921 aatatatatg aaaatatata gtgatgcata tgaagataaa gagaaatata agattttgtt
     7981 aaaaataggt actacagaaa aagatataaa tagagctgta cttaaagaag tagctatttt
     8041 ttatactttg ccaatgttaa gtgcaactat atctagttat tttgcattga gattagcagg
     8101 agatttattg atgaccgatt tatttggtat atatatacta agtttagtga tatgtttgat
     8161 aatattcata atatatggag cagtatctgt taataagttt aagagtgtag tgtataaaaa
     8221 ttagttgata tattaataaa gagaaaggag attcttgtaa taagagtctc cttgttgatt
     8281 aactaaataa tttgggtaat aaattcatat aaaatatgtt atgatattaa taattcatag
     8341 catagtgaaa tattaggata atggagaata tacaaatgta tagaatttta ttagtagagg
     8401 acgatattga tttatcaaaa gagattgcat tagcatttga aaaatgggga tttaaagtag
     8461 aattaattga tgattttgag gtagtattag atgaatttat agataaaaaa ccagatgtag
     8521 tacttttaga tgtaaattta ccattatata atggatttta ctggtgcgaa aaaataagag
     8581 ctatttcaaa tgtaccatta atatttttat catcaagaga ttctgatatg gatttaataa
     8641 tgggtataaa taatggagca gatgattaca ttaccaaacc attttctata gaaatattgg
     8701 ttaccaaaat aaatggtata ataagaagag tctataatta tagtgattct aatagtattt
     8761 tgtattgtga agatttgatg tttgatgtgg ggaaagggat tataaaacat aagtataagg
     8821 ataaaagtat agagttgact aaaaacgaaa taaaaatttt aacattatta ttaaaaaata
     8881 agaatagggt tgtatcaagg gaaagtctta tgatgactct atgggataat gatgagtttg
     8941 tgacagacaa tgcacttact gtaaatatga atagattgag aagtaaagtt aaagaactag
     9001 gttttgatga ttttataaaa actaagaagg ggataggata tataatacaa tgctagtaaa
     9061 atatttgaaa ggtaggtcta agtatattgc tctaccttta gttgtagtta gtataataaa
     9121 tatctactta tttgctatag atatttttaa aaataaatat tcagagttaa tatatttaga
     9181 ttttttagtg ctgtttataa taatggtatt ttttgttata gattatataa attttagaaa
     9241 tagttacaca aatttatata attgtataga aaatagtggt gaaatagaca gttatttagt
     9301 tgatggacaa tcttttgaag aaaatctaat aaaagatatt atagaaaact taaaaataaa
     9361 aaataatagt gacatagaga cttataaaca atcattaaaa gaattggatg agtacatagc
     9421 taaatgggtt catgaaataa aaattccaat atcttcttta agtataatta cagataggtt
     9481 aagtagcata gaagatagtt tggatattaa gaatcaggtt gcaaagataa attttttagt
     9541 aaactctatt ttgtatagta gtagaagcaa cactatgttt gaagatgtat ttataaataa
     9601 atttaattta gaaaaattgg taaaaatgtc tataaaaaat aattcatttt tgcttataaa
     9661 aaataatata gaagttagtt tgaatgaatt agaaaatgat gtttacacag attctaaatg
     9721 catgtcatat gttttagacc aaattattaa taatgctata aagtattcaa aggaagttgg
     9781 taagattgaa tttaattcaa aaaagttaga aaatggagtg gtattatcta taaaagattt
     9841 tggtataggt ataaatgagg aagatatatc tagggttttt gacaagggat ttacggggaa
     9901 aaatggaaga aatcaattgt ataagtctac tggaatgggg atgtattttg taaagaaaat
     9961 gatagatagt ttagggcatg agattgaggt atgttcggaa aatggttcat atacaatttt
    10021 taatatctat ttttatgata tatcagatta tttaagccta gatagttagg ttattagact
    10081 ataaaatcta tcatcatata caaatcgaca tatacaataa gaataaattt agttcttgaa
    10141 aatagtgaag atttaattag tgccttaaat gatgctctat ctatattata aaataataat
    10201 tattatatat gaaaaacata tcagatatgt acctggtatg ttttttattt gtagtaacta
    10261 ttttaatatg gtttttggag agagaatatg ttttgtttgt gaaaaaaaat tacatggtat
    10321 tacattgaca ctgaaaataa attaatgtaa gatttagcta aggtaaaaaa cgtgtataag
    10381 attaactcaa ttaatttcat aagagtaaat tatcgtaatt aaagatgaaa taagattaat
    10441 tctaaaatta ttaaacttaa taccttgatt tggggaggaa taactataaa cttaaaagga
    10501 tgataactat gtatagtgta ggtttaatgt caggaacttc cttagatgga gttgatgcag
    10561 tactagctga aattagtggg aatggtagaa atactaaagt taagcaaata gaatttataa
    10621 ctctagaaat tcctaaagat ataaaagacg aaataagaaa gtgctgtata gaagaggaat
    10681 cgagtgttga tttaatctgt agcttaaatt ttaagcttgg atatttattt agtaaagctg
    10741 taaaatcagt gtgtcataaa gcaaactttc atatagcaaa tttggatttt atcgcatctc
    10801 atggtcaaac aatctttcat ataccaagaa gttataataa ttttgtatct tcaacgcttc
    10861 aaataggtga accagcagtt atagcatatg aaacaaatac aaaggtaata tctaatttta
    10921 gagtaatgga tatagcagca ggtggagaag gtgctccatt agtaccatat agtgaatttt
    10981 tattatatag tgataacaat aaaaatttag cacttcaaaa tattggtgga ataggaaata
    11041 ttactataat acctaaatca tgtaacatag atgatgtatt tgcatttgat acaggaccag
    11101 gaaatatgat aatagatgga gtatgtcaaa gattatttaa tagaaagtat gataaaaatg
    11161 gatactttgc atcaaaaggt aaaattaatg aagaaatgtt aaaagatttg atgagtcata
    11221 agtatcttag tcaagcgcca cctaaaagca ctggaagaga gatatttgga caagtctatt
    11281 tggataacat gcttaacaaa tataaacatg tagataaata tgatttgata gcaacagtaa
    11341 ctatgtttac ggcaaaagct atatattata attataaaaa cttcatactt ccaaaggtta
    11401 atgtagatac tttattaatt ggaggtggag gagctcataa tctaacttta ataggatata
    11461 taaaagagtt attattagag gttgaagtat taactcaaga tgaatatggg tattcttctg
    11521 atgcaaagga ggctttggca tttgtaatct tgggcaatga aacattaaat aattcttttt
    11581 ctaatgtaat atctgcaaca ggggctaaaa ataaagttat tttagggaat ataacaccta
    11641 aaccttttgg aggtaaatga catgtctaat aataaaaaaa agaaaagaaa gaaaaataat
    11701 aattataaaa atgcaaaaag tacaaaaagt aataaaaata ctaagaaaga aatggagaaa
    11761 aatcaaagaa cagattataa tattccaact ataagtttta aaagtggatt aatactaata
    11821 gtttcatctc ttgtaagtac tgtagttttt cctttgttat tatctatatt tggaatagat
    11881 aatagatttg gagttgttat aggaaattca ttaattacat cttttgcagt tgcatataca
    11941 agatactttg ttgaaactaa gagaggtttt tctttgggat tttttagact gtatatttta
    12001 tttgctttat catttgcaat aataggttac ttctggatat ataaaggaat acatatttaa
    12061 aaaagtattg agactatttt atgttaataa taagatagtc ttttttcttt tttaagaaaa
    12121 gatatgagta ttttaagtag tgtgtaattt tagacaaaag aatgattaga taaataaagc
    12181 aattaaagca agatataaat tcatgaaata aaattacaaa taaaaatgta aaatatgaaa
    12241 aaatatatac aaacgtattc ctgtaatcaa aaatacatgc tataatgatt gaaaagaaat
    12301 aaaaaaacag ggggaatcaa tatgagagta ttatttgttt gttcatcagg gatgtcaagt
    12361 gctatagcag ttaatgcatt acaaaaagaa ggagcaaaaa atggaataga tatagatgtt
    12421 ttggctgttg gtactcaaga gtttgaggat gaagttaaaa atggatggga tattgctatg
    12481 gtagctccac aagtcagaca tagatttgac tatttaaaag cttttgctga tgaggttagt
    12541 gtaccatgtg cattaataca agcacaagct tatagtccat taggaggacc aaagttatta
    12601 aaacaagttc aagaactgtt gagtaaatag tagttgcagt gttgttagat tagaagaaag
    12661 ttgattttat aaaaatttta atgataataa aaataaatat taattaagga gagaaaagat
    12721 gctagataaa tttacagaat ttttagacac taaattatca acttctatgg ccagattggc
    12781 agaacaacgt cacttaagag ctgtaagaga tggaatcgta gcttcattac cacttataat
    12841 agttgggtca tttttcttga tattagcatt tcctccatta ccagattcat ggggggtaac
    12901 tcagtatcta aaggaaaatg cagttacaat attactacca tatagaatga cattttttat
    12961 tatatcagta tattctacat ttggcatagg ttatagtttg gcaaagtcat ataagttaga
    13021 tggattgaca ggaggtttat tagctacagc tacattcctt ttgacaatag ttcctactgc
    13081 aatagagggt cttacccctg atttattaaa gatagttgaa aagagtccag aacttttagc
    13141 ttggtataat tcaattcctc aaggatggca aatgccaatg gctaatcttg gtggaggttc
    13201 aatgtttgtt gctattgtaa catctataat aggtgttgag atttatagat ttactgacaa
    13261 atctaaattc aagataacca tgccagaagc agttccagca tcagtcacaa ggtcttttga
    13321 agctctgaca ccagccgcat taataataat tttaatggct attgttactt atttcctagg
    13381 gtttgattgg catggcttca tagctaaatt agtaactcca ttagtttcag ccactgacac
    13441 attaccatca gtaattatac taatattttt aataacgttt ttttggtcat ttggaataca
    13501 tggtgtatca ataataggtt cattggcaag acctttatgg atacaattat tagatgctaa
    13561 tacagcttca tatgctgcgg gtcaagcagt tccaggtatt gctgctgaac ccttttttca
    13621 atggtttata tggattggag gttctggagc tacaataggc ttagctttac ttcttgcatt
    13681 tagagcaaaa tcagcttatg gaaagtcttt aggaaaagca actgtaatac cagcaatatt
    13741 caatataaat gaaccaataa tatttggagc accaatagta ttaaacccaa tattaataat
    13801 accatttgtt gtagcaccaa tagtaatggc aacagtttca tggtttgcta tgagtgctaa
    13861 tttagtatcc agagtagtag ttactgctcc ttggacatta ccaggaccag taggggcata
    13921 tttagccact ggtggagatt ggagagcttc agttcttaac ttattatgta tagctatatc
    13981 aataataatt tactatccat ttgtcaagat gtacgataag agattgcttc atgaagaaaa
    14041 agttgaagag caagaagctt attaattaga aactatattg gaaattaaaa actatattag
    14101 attagtataa atttcaaagt ataacagtta tgaaataagt aacgaagata ttttgttgat
    14161 tgtttataaa aaaagaaaag gtaaactttt ctttttttat aaatatatat gaaacttttt
    14221 ttcaccaaat ttttaaaaaa gtttcgtgta attcgaaata aatttaaaca catataggat
    14281 ataaggagat gacacagatg ggaagattag gaatatcaat ttatccagaa aaaacaacat
    14341 ctaaggaaat atatgattat atagataaag ctgctgaaaa tggatttaca cgtatatttt
    14401 catgtttatt atcagttaaa gatactaaag aaaatataat taaaaaattt aaacgtataa
    14461 atgaatacgc taagtttaaa ggttttgagg taatacttga tgtaaatccc agagtatttg
    14521 gtgaattaga aataagctac aaagatttga ctttttttaa aaagacgaag gcagacggaa
    14581 taaggcttga tatgggattt acaggttctg aagaggcatt aatgactttt aatcctcaaa
    14641 atcttaaaat agaaatcaat atgagtaata atactaatta tatagataca ataatggact
    14701 accaaccaaa taaagaaaat ttgataggat gtcataactt ttatccacat agatatagtg
    14761 gattaaattt aaaacacttt ataaaatgta cagaaagatt taataagtat ggattaagaa
    14821 cagcagtttt tgtaacaagt caaaatgaaa ctacatttgg accatggcct gtaactgatg
    14881 ggcttccaac aattgagatg cacagaaatc tacctataga tattcaagta aagcatataa
    14941 tagctatgaa taatataaat gatataataa tatcaaattg ttatccaaca tatgaggagc
    15001 taaaggtatt aggttctatg agaaaagata tggttacatt tgatgtagca atagaggaaa
    15061 atatacgaga agttgagaag aagatactct ttgaagaaat acattttaat agaggggaca
    15121 tatctgatag tctgataagg tctactcaat ctcgtgttaa atataaagga tacaaatttg
    15181 aactatttaa tactcctaaa ataataaaaa gaggagatat agtaattgaa agtagtgaat
    15241 atggtcatta tgcaggggag ttacaaatag ccttgactga tatgaaaaac agtggtaaat
    15301 cgaatgtagt aggatatata agaaaagaag aattatttat attagattat attaaaccat
    15361 ggcaaaagtt taattttaaa ccctcaacaa aataatttat tatgaaaata ttaatttatt
    15421 ataaaaatat aattatgaaa gttggaatta aaatgtgttt aaaaatttaa tattaagaaa
    15481 atgaggaaat gatatgttaa taaaaacttt agaaaattta gttacagaag gtagaaataa
    15541 gaatacattg caaatagata aagaagatac attaggcata atagaattaa taaataacga
    15601 agataaaact gtagcctatg ctgttgaaga acaaaaggag agtatagcaa aagctgttaa
    15661 tataatagta gatagaatga agcagggagg tagattattc tatataggag caggtaccag
    15721 tgggaggata ggaatactag atgccacaga atgtcctcca acttacggag tggattttga
    15781 gcttgttcaa gctataatag ctggtggaaa tcaagcaata tttaaagcaa tagaaggtgc
    15841 tgaagatgat aaagaacttg gaaagcaaga tataatagat agaggagtta catctaagga
    15901 tgttatatgt ggtatagctg catcaggaag gactccttat gttataggtg caatggagta
    15961 tgctaaggaa ttggggtgtg cagtactatc aataactatg aatccgaata gtgaaatgag
    16021 taaaaaagcg gatttaccaa taaacattat agtaggggca gaagtaataa tgggctcaac
    16081 tagaatgaaa agtggtacag ctcaaaaaat ggtatgtaat atgttaacaa cagcttctat
    16141 ggttaagatg ggaaaagtat actcaaactt gatggtagat gttaagacat caaatgaaaa
    16201 gttggttgaa agagcaaaaa gaataataat gatagctaca aatgtaaaat atgacgttgc
    16261 agaaaaattc ttagaagaag cagataatag cgttaaattg gctatattta tgataaaaag
    16321 tggtttagat aaggatagtg caaagagtat cctagataga caagaaggtt atataagtga
    16381 agctttaaaa tcaatagaaa aattgtaaaa taaataatag tactgataac ctttcaatat
    16441 taaaaaaaca gcattggaag gttattttta gatattaagt attgaaagaa ttattaaaga
    16501 tattgtagga ggatagtatg tattttttag gtgtagatgg tggaggaacg aaaacaacat
    16561 ttacattagt agatgaagaa ttaaatatag taggaaccat aaccaaagga acttgtcatt
    16621 ataatcaaat aggatttgat aatctaacga aattattaat aactgggttg gaagaagttt
    16681 gtaaagatgc taagataaat gtagaagaaa taacgtatgc atttgttgga cttgctggat
    16741 atggaaaaat aaaagaagta ttgtatgcat tagaagtagc aacaaaaaat gcatatagtc
    16801 atataaatta tactctaggt aatgatgtag aaatagcatt agcaggttct ttaaatgggg
    16861 aaaaaggtat aaatataatt gcaggtactg gttcaatagc acaggcttta gataaagatg
    16921 ggaatcttca cagatgtgga ggatggggct atgtattagg agatgaaggt tcagcgtatt
    16981 atataggaat ggctacctta aaaatgttca caatgcagtc tgatggaagg tgttcaaaaa
    17041 caaaattata tgatttaata aaaagacatc ttaatataga gaatgattat gacataataa
    17101 aatatgtaaa tgatgaaata caaggagata gaatagaaat agcaaagttt gctacgatat
    17161 gtttaaaagc agttattgaa ggtgataata ctgctagtaa aatatttgat gatgcagctt
    17221 atgaattaag taggcttata ataggattag atcatcattt tgaacaaggt acaaagataa
    17281 aggtatctta ttcaggtgga gtgtttaagt caggagattt aatattagag ccacttaaaa
    17341 aatatttgaa taaaacgaga tttggtatag tgagaccagt gttgactcca gatttaggag
    17401 catgtttact tgctaagaag aaatattata gtaaataggt agaggtggta gagtatgagt
    17461 tgcattttaa aattaaaaca gatatatgaa gatttaacgg aagtagataa aaaaatagct
    17521 gattacatac ttaataatac agaggctata agtaagcttt ctgtatcaga attggcgtca
    17581 aactcaaaaa caagtacagc aagtatagtt aggttttcta gaaaaatggg atattcgggt
    17641 tttggggatt taaaaattga gatagctaaa gatttgatgg gcaaagaaaa tgaatacaca
    17701 tatgtagatg aaaactatga ttgtaatata gatagtgtta taaacaaaat tacaaacaaa
    17761 aacatagaga ctatcaacca aacaaggttg ttgaatgaaa aagatataat aaaagaagca
    17821 gttgaacaga taataaaggc taaaaatgta tatatatttg gagtaggtgg ttcggcttta
    17881 gttgcactag atttacagat gaaactttta aggataaata aacaggcatt tacctcttta
    17941 gatagtcata cacaacttat ggtatcttca aatgtagata aagaagatat agctatagca
    18001 atatcttact ctggagaatc aaaagaagta ataaagtcta tagaaaatgc aaaattaaaa
    18061 ggatgtaagg tcatttgtat aactaaatat agtgacaatc atcttagtaa gattagtgat
    18121 ttaaagctag tagtaccaaa tatagaaaaa agacttagag aaggagctat ttcttcaaga
    18181 atagcaatgc ttacgttgat agatataata tatatttcta taattcaaga aaacttaaat
    18241 gaggcagaac agaaactaca agaaacaaaa aggatacttg attatttaaa tattaataat
    18301 gcttaaaact aggttatata tcaactaaac aaataagtta ggctataagc gtaagtcaat
    18361 aagaaataga taaatatcta aaatattgac ttacttaata ataatatata gcatttttat
    18421 aatacaaatg agttgtctta aaattgattt tttttaatca aattttaagt tcaactcctt
    18481 ttttatattt ttatagatat atcctatatt ttcccagaaa acgtattgaa tatacaaatt
    18541 ataagaagta taatgttgtt gggagagaat gtatgaaatg tatacaaagt tgattttaac
    18601 tgtattattg ataattctct ttactcttac cattattaat gattgaagac aatggtattt
    18661 attataggtc aaagattccg aatgtattaa tcatcatcat tttctcaaac taacagcaaa
    18721 caccctgatt tgcgagttgt attgtataca aattatctag aaaatatcta ttagattttc
    18781 catgaaattt aaatatttca cgaatgtatg aatggagaaa tatacatgtt gacaatccac
    18841 ctttttaaat cctaaattaa ttttattaat ggtagagaat aggaagtaac aatgttttat
    18901 aaaggagaat attaagtatg aagaaaatta ggaagttagc taccaagtta ttgattacaa
    18961 caatagtttt aatctcaggt atgagtatga cagtttatgg tatgacagcc aaagaggtaa
    19021 cagcaaaagc accaaaaagc tatgtcactg gaaccaatag cgtatatggt ccaaagctgt
    19081 cacaagccca actaaactca gtagcacaag caacagcaga ttttatgaac aagaaaatca
    19141 caaagaatat gaccacagat gctaagattt tagttgccta caatcacatt aaaaataaca
    19201 caacatatgt tgactggaat gcagtagagg gagcaaatac tgcctataca ttggttacta
    19261 aaaaaggtgc atgctcagga atggctagga gcatgaaagc tttgtgtgat gctatgggaa
    19321 ttgaatctta ttatgtacac agcacatcaa atgaccacca gtggaatctt atccgttttg
    19381 gtgatgggcg tttgtatcat gtagatattg atgctaataa atcagcagga aaggatatca
    19441 tatacaagag tcttagccac ccattaccat ttgataagac agcatatcca aaagttggtg
    19501 caatagtaca gacggtaaaa ccagaacaga cacagaaacc agcaccacct gggaagcaca
    19561 ctatgttacc acatcaagaa ggggcaaagg ctgttggaaa ttttggaaaa aaaggagaag
    19621 atataaatga aaaacatgta atgtatgaag gtgttagatg tagtgtagtt aattcatcag
    19681 taaatacaat tacatttaca ggtagtgaat caaatttaga cccatcaaat cttacgattg
    19741 ctgtaatagg atttatgaac acaccatatg ggatgtttgc attagcacca gagggtaaac
    19801 ctgttgaata tccttttaaa tatgataagc cattttcttt agattttagt ggtaaaaatc
    19861 gttctggtgc agaattagcg aagtactgta aaaaaaatga tgtgactatt aaaatagaaa
    19921 tcactgatga aaaagctaat attggacttg gtcaaataat gtatgttcga tatgaataat
    19981 gatttaatca tgacagtaga ataagttata aacattgcgc cctcttacta ggggcgcatt
    20041 taatattata tctgatattt tgtagtcagg tcaactgcta gatttctccc ctttttattt
    20101 ataaaaatta ttatctctaa gcatctgagc atgtttacga ttaactaaaa actgttttaa
    20161 ctcagtcaag cataggaata aaatagctct attttcaaac tctgttctgt caacaggtag
    20221 aggctggttt ttcatactaa gcattagtct ctttaattct tcaagaagaa gattgcctgt
    20281 ttcagcttca aattcagtat aacctatctt atgtataaaa gcagatagaa tatgagcttg
    20341 tggtggtgta gaatttagtt gagatacata actatacaaa ttttctaaga tatcccgttg
    20401 actttttata atatccatat gctctaagaa gaatctagta tcacttaata aagtattatt
    20461 aatattatcg taagtttcag aaatactgct gtcaataagt gaatttaatg tatataaatc
    20521 tctactatat ccattttctt ttttaggatt tacaataata tctgcaatgt ctataagtac
    20581 aatactcact tcttcctgta actttttttg aacttcatgg attttatgta tatttgttgg
    20641 aatatacata tttattaaga cacctattcc agttcctact gcaaatattc cagcttcatt
    20701 taaaatccat ttgatagaag tttctccttg aaggtagtaa tgggatgcta tgactacaca
    20761 catagcaata acatcttgaa tatttaacaa aaaacaaagt ggtataatta taaatataaa
    20821 tattgaaaat gaagtcaaat tatatcctaa aatattaaaa cataaatatg aaaaaatagt
    20881 acataaaaga aaaccataaa tctttccaat ggagccttta agagtttctc ttttagtgtc
    20941 ctttactgta agtagtacta taactccagc aaccattgaa tattctaatt ttaatagcca
    21001 agcaataaat atagcaagag aacatccaag agataatttt attactttaa gtgaattaat
    21061 attattcaaa tgcaaacctc ctgtaaatca atataataaa attctaacat aaaactaata
    21121 ttgcttgaaa ggtaagtaac tatacttaat tattttattg ccctatttgt ttagatacaa
    21181 tcaatataat tttattttat gaaaattttt tgtaaaattt taaatttaga gactaacatg
    21241 agaccacaaa gaggaagttt attataggaa gaaaacttaa aattgatttt aagcagcaaa
    21301 tgaaaataat caaatatgta tatactatta ttaaaaaata aaattatata gttaatataa
    21361 ttttataatt ggtttagtat agagattttt aacttttatg ttatactatt tataaaagta
    21421 ggtataaaga ttaaaataca taatcaaata tgtataagag gttttaaata gtattgttaa
    21481 tgaacttgat tattgtttat aattacaaat tggaggagat agacatgaaa attactgaag
    21541 gatatatgcc ttttaaaggt tttaaaacgt attaccgtat agtaggagaa aatacggaag
    21601 ggaagaagcc attagtatta cttcatggag gtccaggttc tacacacaac tattttgagg
    21661 tattggataa gatagctgaa agtggtagac aagtaataat gtatgaccaa attggttgtg
    21721 gaaattcatt tgtagaaggg catcctgagc tatttaatgc agatacttgg atagaggagc
    21781 ttattgaact aagaaaacat ctaggacttg atgaaattca tctgttaggt caatcttggg
    21841 gaggtatgca ggctatttgg tatgctattg aatataaacc aaaaggtatt aagtcttaca
    21901 tcctttcatc tactctttct tctgcaaaac tttgggagaa agaacaaaaa agaagaatat
    21961 catatatgag tgaggttgac caaaaagcat tacttgatgc agtaaatact ggagattact
    22021 ctagcaaaga atataatgat gcattagaaa gatttatgga aatgtattgt gcaggtgaag
    22081 tgacagaaga ttctccagag tgcctgagaa gacctaaaaa atcaggttct gaagcatata
    22141 ttgtaggatg gggacaaaat gaattttctc ctacaggaac tctttctgga tatgaattta
    22201 cagatagatt acatgaaata aaagagcctt gtctagtaac tagtggagca atagatttat
    22261 gttcaccata tattgcaaag actatgtatg atagaatacc taatagtaag tgggaattat
    22321 ttgagtattc aagacatatg ccattcgttg aagaaaatga gaaatatata aaagtcttaa
    22381 ctgagtggtt gaatgcgaac gattaaaaac tatataaatt tatataaaaa aatgagtgtc
    22441 tcaaaatgaa ctttttagtt cattaggcac tctttttgca tgaaatcaaa tatattttca
    22501 ttatttgaac gataaaatga agcttatatc tattttggga aagtaatttt ttattactag
    22561 cgttagattc taatctaatt gtatacttat tgaaataata gaaatatatt ggtgtttata
    22621 acttaatttt attgtttttt agaaattttt ttatatattg gtataattat tctgtttaaa
    22681 tttgattaaa aagtatatta attataataa gaaacaatta tataaatagg aatagattat
    22741 tataattaat atagatataa aaataattat agaaggaaag gcacattatg gaaaaattga
    22801 gagttattga aatgataaaa aataatccca atattatagc tacaatagat aatccaactg
    22861 atgaaatgaa attgttagct attaaagaaa atgggcttgt actagaatat ataaataatc
    22921 caaccagaga gatgcaagaa ctagcaatag ataataatat tagagcaatt aaatttatag
    22981 ataatccaac agaagatatg atgataaaag cagtaaatga gggatggagt atactggatt
    23041 atatcaaaaa cccaactgat aaagttattg aaatggctat aaatcaagca ggatgggcaa
    23101 ttaaatatgc taaaaatcca agtgaagaat tacaactatt agcagtaaga aaaaattatg
    23161 attcgataaa gtttataaaa caaccttatg aaagtgtaca agaggaggca gtgagaataa
    23221 gttatgatgc cttgagatat atagattcac ctagttacaa tgcagaactt atagctataa
    23281 aaaataatga agcagctatt agctttataa ctgatttaga taaaaataaa atgttggaat
    23341 ttttaaaagt gaatatttta gtgattaagt acattctgaa aaaagtatct aaagatgaat
    23401 tagagagtgt attaaaagaa gtattatcca aagaagatgt tgaggaaaag tatattagag
    23461 attttttgaa ttgtagtgtt atagatagaa atagcgaaag tataccaata gataaaatta
    23521 tgtttattta taaatatgga agtaagacag caaaaaaaat agctgtagat gagaaattaa
    23581 agattatgta ggagagagaa aaatgaattt tatagacaca ttaaaaagcg ttgacttaat
    23641 attatccact ggagaagtac ctttaatagt aggtgaaagt ggaataggaa aaacagcttt
    23701 agcaaataag cttgccaaag aaaatgattg gagtttaatt gttattgatg gaaacttact
    23761 taaagagggt gaaataggtg gtcttccaac tatagaatct tatgtaggag ttaattctaa
    23821 tggatataag actgaaaaga aaacaacagt gtatgctgtt cataacaaat taagagaaat
    23881 tgatgaagag atatctaaaa gtaagacagt tcttttattt atagatgaga taaatcgttg
    23941 tgagcataca gtacagcaag aacttatgaa tttaatttta aatagagaaa ttaatggata
    24001 taaactgcat gatgatgtaa agattttagc agcaatgaat ccatcaagta aatatggttc
    24061 agattttgat tatcaagtgg tagatatgga tgcagcccaa gaaaataggt ttgtttggtt
    24121 aaatatggag agtgaccata cacaatggat aaaatgggca atagatgagg gtattgaaag
    24181 aaaagttatc gagtttattt ccacttttcc agaatatctg cataaaataa atgaagatga
    24241 tgtaagagcc actcctagaa gttatgagag agtatctaaa atttataaag tttataaaga
    24301 aaaaaataat tctattccta gagcagtatt tttaaatgtt gtaaagggta atgtaggtaa
    24361 agttattgca gaagaattta taagttttat tgaatcaaac tctgaaccat taatatccta
    24421 tgaagatgtt tttttaggag aatctattga tgagtctatt gtagaaagag taaaaaatga
    24481 gagtcataca agactttatc tatcagctat gaacattcta aaagacttag aattaaatat
    24541 taagaatgat aaatatgaat caaaccacta tattaataga tttattgaat ttttaaaaat
    24601 gtatcctgta gatttaatga taggtattat gaaggatatt agaaacagtt atattgaagt
    24661 atataaaaaa gctatagaaa atgaagagtt tgtaaaatca tattttgaat cttatagttt
    24721 aataagggga taatgtatgg aaaattattt tgataaacaa gcaaaagagc tttataataa
    24781 agcaagtgaa attataaata ctcattctat gctaaaggca aatcgtgcta atgagaaatt
    24841 tgatatagat ataccacaag attttaagta tgagtttttt agtcttgtag ataaagtgaa
    24901 tttaagtctt atggaagaag aagataattt ttatggatat tttttgtttc agatgtcaag
    24961 agaaataaga tttgatataa gtagtcctac aggtgtgaat tttaagggag ctaaatatgt
    25021 tatatatttt aatccaataa tctttttaac gcttgacata aagcaaatgg agactacaat
    25081 taaacatgaa attcatcata tattatctat gcatttgatg agagcaaagg agttaaaagg
    25141 aaagtatagt acattagcaa ttaatatggc aatggatata gtagtaaata aatttctaaa
    25201 caatttacca ccatatgcaa ctaccttaga gtgggtaaat ttaaagtatt ccttaaaact
    25261 tgaaccatat gaaccttttg aatattatgt agaaaagatt cagactgaat tagatttgat
    25321 ggaagtggat gaagatggtg aggaagatga tagtgataaa tatggagata tagaaattga
    25381 ttatagcccc gaaagaactc atgatatatg ggatgaatcc aatgagatag atgaaaatac
    25441 ccttagagaa tttactgaaa aatttattaa cagttctcaa aaaggtaaag ttcctgctta
    25501 tttagaaagt atgatatcat cacttaaaaa tagtaagggt gaattgccat ggaatttata
    25561 tcttaataga ataatgggaa cagttgaaaa taataaaaag aagactataa caagaagaaa
    25621 tagaagacaa ccaaatcgtt tagatttgag gggagaactt agaagtcata agtcagaaat
    25681 agcagttgca cttgatgtaa gtggaagtat tagtgatgaa gagtttaaac aagctattaa
    25741 agaagttctt agtatagtga aaaactataa tcatgaaatt acaattatag aatgtgataa
    25801 tgaaattaga cgtgtatata aagtgagaac tgaaaaagat ataaaagata gaattaggat
    25861 aggtggaggc actagatttt ctccagtctt tgagtatgcg aataataaaa agattaattt
    25921 gttagtctac tttactgatg gtaaaggtga aaataaacta caagtaatac ctagagggta
    25981 taaaatttta tgggttattt ctggaagagg agataaaatt tcattaaaag aaccttatgg
    26041 agcagttaaa aaacttacta aggttaaaac aaatgatgaa atattagaaa taaatgatgt
    26101 aagaaatgat gggtactcaa tgaatagcca agcaccaatt ttataggtat tcacaggaga
    26161 taagctttat taatttattt tgatatgctt gttataaaaa tctaaaaaat acattaatca
    26221 atggttaatg tattttttaa ttttaataat ataagtttta cttacattta agtcttaaaa
    26281 tgtgttaaaa tataatatat agcagatagt tttggcaagg tggatttagt tgtcaaaact
    26341 atttttttta ttaaggatgg tgatgttttt gtttaagatt gatgattata taatgtatgg
    26401 tatgacagga gtctgcaaag tcatggatat aacaaatgaa cgatttacaa atggaataaa
    26461 aaaagagtat tatgtattga gtcctattta ttctaataat acagtaataa aaattcctgt
    26521 agataatgaa aaagtaccaa tgagaaagtt actttctaaa attaatgtgc tttcattaat
    26581 aaatgatatt cctaatatgg atacatcatg gatagataat gaaaaattga gaagtgagca
    26641 atttaaaaaa atactcagag gtggtaaatg tgaagagtta ataaaattag taagaagtat
    26701 agataataac agagagtatg taaaatctat tggtaaaaaa acacatcaag cagatgataa
    26761 tataatgaaa gaagctgaaa ggttattaaa tgaagaattt gcaactattt tagatatttc
    26821 tcctaatgaa gttagttcat atatttcaag tcatatacct caataaagtg ttttagtatc
    26881 ctttatttaa tctattttaa gtattattta tgtatgctgg ttttggtaaa ataaaatata
    26941 taattaaatt atataaaaaa taataatgat tgtattgaag aattacagaa tagtgcggag
    27001 agcctactcc tttaggtgtg agatggatat cacttaaaga tttaaattgt tattgtagat
    27061 gtttttaata taaaacaaga ttaataagtg tattgtaagc tttaagtgaa attgatacta
    27121 taatgagtta cttagatttt aaaatatata atattataaa aaatcctttt aggttttgaa
    27181 tgaataagat ggatactatg ttgaatagaa tccatcttat ttaagtttta tttatgttgt
    27241 ttatttgtaa gcattatttt tgtacatgaa aacacagtgt aaaaatatgc ttagaattat
    27301 gaagttttat taaaaagata ttcgacaaaa taagactaaa aatattctta aacttataac
    27361 taagataaat attgtatatt tatagtgtag cctgaaaaat tgtgaatgta cgaaatttat
    27421 ttagtatttt attgaaacaa agtagaagaa gtactatagt ttatctttga atagattgtt
    27481 attttttagt gaaaaagtta ttaggttaat acataaatat aatagcttat aaatagttta
    27541 agcatgttaa aaaagtagtt attagagata tatagttaca ttgcttataa gata
//
LOCUS       DAKBNN010000028        27200 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000028 DAKBNN010000000
VERSION     DAKBNN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.028"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            332..787
                     /gene="smpB"
                     /locus_tag="OUB02_001092"
     CDS             332..787
                     /gene="smpB"
                     /locus_tag="OUB02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCT8884868.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            945..1294
                     /gene="ssrA"
                     /locus_tag="OUB02_001093"
     tmRNA           945..1294
                     /gene="ssrA"
                     /locus_tag="OUB02_001093"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1960..3219)
                     /locus_tag="OUB02_001094"
     CDS             complement(1960..3219)
                     /locus_tag="OUB02_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCT8884869.1"
                     /translation="MIECIPYPGYRDRDGYGQINSNKEGTLRAARIVLEERLQRPIRN
                     GYQAHHTCYNRCCVNPSHIEERKVSENILDKYFNDRPEINEIVLTKESVYLTKYLLNN
                     MNNSEFNYSIEEIAKFLSIPLELLLYIKKGSVWNYIYLTNAEKVEALHKLEIFFPKEV
                     CIEANWGSEKYCPVVHKNNKQIKASRYSYDTYTNTSSSGKYILHNCDNPLCIRASHLR
                     TGDQKDNMNDKVQRRRTNRDYYLYNQEIIKKIVQLRDVENYSFSKIGREIAILVKRDK
                     PYDHKTVISIYKNYKYTRREELKEKPPTQRELLNKHIDTLIDLKHNYNYSLRQISHKL
                     GSNLIGKPFDLNVIGQVYKEHISKQNLIPPKSSIKLLEENITEILSMLDCNISLREIA
                     NRLTKKLNPNKPFHHNQITKVVNKYKK"
     gene            complement(3318..4484)
                     /locus_tag="OUB02_001095"
     CDS             complement(3318..4484)
                     /locus_tag="OUB02_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT8884870.1"
                     /translation="MATKIANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFKYRIKDLKPKSFERYESIYRNYI
                     KDTDLVNVKLNDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKIEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDINFKNSNLTVKRTLQRTYFIDKNGDRELKVLEQEPKTSNSYRTVPIPKNILNKLKE
                     HKVSQNTDILKAGELYRNDNYVFCNKLGVPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENNVPPKAVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(4474..5226)
                     /locus_tag="OUB02_001096"
     CDS             complement(4474..5226)
                     /locus_tag="OUB02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCT8884871.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKEKLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEPTLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     GDEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            complement(5318..5808)
                     /locus_tag="OUB02_001097"
                     /pseudo
     CDS             complement(5318..5808)
                     /locus_tag="OUB02_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(6059..6823)
                     /locus_tag="OUB02_001098"
     CDS             complement(6059..6823)
                     /locus_tag="OUB02_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8884872.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISDISRNALINYENDKRIPNI
                     DTLSKLAKALKIEKTIFYFDIENEVITNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            6959..7607
                     /locus_tag="OUB02_001099"
                     /pseudo
     CDS             6959..7607
                     /locus_tag="OUB02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891536.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            8231..11710
                     /locus_tag="OUB02_001100"
     CDS             8231..11710
                     /locus_tag="OUB02_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCT8884873.1"
                     /translation="MGKIEPNQEDRIYEDLYQGNSAEGKVCEWIEEAINDDCYVGQHR
                     FKIETRECDIAMVIPNRGILIIEVKGYTVEMIDHMSSNFIIHVKKEYENERFKTGERH
                     PAEQANDYVFTMMRDFSGFLKRNNICLPICKIGRMAAMPNMTDKEYEELYLDYCYSTK
                     LVITKDWFKNKNIFYHRFINALNIIFNSISSEVDFDDSYYNIARRYILLGERKKYVDL
                     CKEQLKREEELGIDFIIDNTNEEVDQLCKSNEVNDEEMELIKFSSVLQEELTESHKLS
                     KIIPYSKLRIVKTGCEIEPLLEICCNERKEGIKQFLILVNFTRDEKEKILLEYEKRFL
                     NKYKENELLFLFNMYFIDLSNDDTSIDNLFSNHDNIEIINGERIEEIVKIKPDNFIVQ
                     EFTPKDLLDQALWNILCIADNFTSFNFDQYIVEHSPLRQNIVVTASAGTGKTYTMVDR
                     VSFVAHMEIIYRNSKESISDLISMITFTNNSTDEMKERLMKHFMQYFELTHNKVYMDL
                     LEQLGRMKIKTIDSFTKYILSKFAHLLGLSNSFSLKSGKFELENILRKYINDEITKEI
                     IDDFHNINIYEIANHILALRSFLINRKIQFDKIKHASIVGPDVGKGTIRMKNFIESVL
                     ISTNSEYDSFNCSQNFISMNELVIQLEKVKNKLIKTSCVNNTKENYEKYLFIDEFQDT
                     DNKQIQLIRFFADYFNIALFVVGDEKQCIYGFRGAERTSFLELTCVDTNFQQRNWISC
                     FLRKNYRTEEPLVKKINEVFSKMPNDFFPYQNKDKLVAQKGIGRIYSGCYEQFKYSEN
                     NLIELVDNCVKRMKKNNEDGQIAILTRNNYEVDQIRALFGENKEYEIKFDNGGLFYNT
                     DAVIDFYKLVEFLMDTEDGAKRYSILETPYSDFRIKESKIKLYQAYEKMDVETFEIIR
                     SGNNHVSLRKYYDVLYNYGKKHPNLKNSPSLKILRMIVNEIKPWEHYVPERYKTVDFN
                     DICNQNKLIEARNEYRQNLFQLFQKLIDDNKTDYLTLNKIHQILTIGIFAMQDIDIDL
                     PKNNFFGEKISVLCTTVHKSKGLQYHTVIMPFTMNDITESRGSTQFIYREDTNEIYMQ
                     LKEADGKCLIYESTNFDCENNKKNETIQSEELRILYVALTRSKKFFYFMVDEERFEKN
                     NTSKKNCWAHYLM"
     gene            11727..14963
                     /locus_tag="OUB02_001101"
     CDS             11727..14963
                     /locus_tag="OUB02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884874.1"
                     /translation="MKIYTYKNSVQLKDAIFWNEIEGAPHFCSSEVLAIGMRKFYGRN
                     TFPCISTIDLLIKELYPNWLKSHSDIERYKDISKLINELNDSSTEGLRQAFVKNKKSI
                     LDSCKYLIETEDLLLKDYYNKNIFNKFSAINDIQEVFKEKILKRLLEQIYIDGNKNYW
                     KPEWIDTLWVKSKLSEVIHKCVIKEISDIVKNTEEKSLNHNYDEGHIFEIFDELFDQH
                     GLFNISDKTLEYWKFNSKDDLLDKIGYSSKELEYVRNLATTECKSYKTIVIHGVYHLN
                     PIHLRLLRDLERDHNVIVLNCYNKEFNHIYEFWNDIYDHLIKIFQIPKNNIKIDSHDS
                     ANHREVGSIFAEVVNGVINKQSNFFKISRNEFGPDLNLNSKDIDELFNHNSSQDIRYI
                     LNKSEAIIQNYEDEDEREYQKILIDTSKELQKELCHRSKILIKYDSTMQFVNKVCNIF
                     DETKNKKGIRSVGRMKEQFYGSKGTEMNKIFQVFYPDEFKVKPFLSYPIGQFIYAIHN
                     MWDLKNHKLILNSRDLAECLNLDVWHDIHPLEVFESLRFYIGIDKEPNGMNVKDFIDR
                     IKKLIYFKERNENKMKNLDNLSYLNLTIDQYNRFIQVIENINEIANKIFCNKSQTSKK
                     HFMDLLEILNDEKAWKPLLEEEKRLMDEIKNQIIHIDDETNKTDLNTIKDTMAYYLDS
                     KEGTDINWLVRDFEQLEGDILSEAANYKNNTLRQKCYHLTMLSNENMINCDIDNLPWP
                     LSDDFIISKNISSFIHTICINYAKYKRCLLFQGLYYLINNPIISLKLSYIENQGEYKH
                     TPYYILNMFMNIGKDFDQSNCRNIESCTSPINKEPKKRFKIDKKWHKDAIESFSCCPY
                     KFMYGYVINDAAKHYKDELQVKMYLGEIIKYYISIAWKKILQYHKKLLLDQKFNLLKK
                     WTAGERLDCLEKSLPETLEPIYGNYFIKSEINKIIVDNLEYLKNKRIDNKRVYFTEEC
                     KIYEVDRELYHCWIGKISNHNLYVTNKNKIINIRNRLNLLNANEYINDYILGINDGWY
                     FKKDNIVVEGGYKVSEHACRYCSYNKICCYQYRIKPIHLLHSKEDLID"
     gene            complement(15075..15458)
                     /locus_tag="OUB02_001102"
     CDS             complement(15075..15458)
                     /locus_tag="OUB02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HCT8884875.1"
                     /translation="MRFKLKTSTQTKEIFEELGSRMNLKPFALCKLAISMSLSYSESI
                     ESYENIDNNGLELNRQTITNQYDTLFKTLFEQHIGRHLSDNEYFPNQVKLHIDRGAIM
                     LKNRYNYSSNLEHFITQIIQEDNNL"
     gene            complement(15461..17620)
                     /locus_tag="OUB02_001103"
     CDS             complement(15461..17620)
                     /locus_tag="OUB02_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8884876.1"
                     /translation="MKINKLVLKNFRSYEEETTFNLNTTSDKNIILIGGKNGAGKSTI
                     FEAIKLCIYGPMAYKYQGFNASYISQVKSNINNNSLINSKIDTLVSVDIEISEGTEEN
                     IYTLIRQWTFKDEKLNETFLVYKNFSTTPLNADELNYFENYITSIISPKIFDFFFFDG
                     EHLYDFFVGKNSNIHLKESLLSLCNFDTFDILKKVLISTNRSNKNVSDEINIVKDSYL
                     NLEMEINTLNSQEEIFSYELQKISNEIEKLQQEQLEVKKEFRKKGGILAEERETLNSR
                     IAELEGKRFIINQNIKEFCNDILPFLIVKKQISNLEKQLKTENDILLYSRLKDKLNIE
                     YIKNLLSDKIMPSALDEIALTVSEALTEDIKPDSYEENFKSIHNLSDDESRKLLSLIN
                     TILNFDNNSIINSFEEHRSISNELADIRHKLNTSLKDESLNTYINDMSNLSSKIDSLL
                     GSKNLLVTSLDKLKLEIAETKSRRDKSKAKYIYLLQHNNIVDISTNLILMLDEIVTTL
                     TETKIKLIQDNFMYIFKKIIQKDNFISHIDINVNFDISLYINKIYNSLELENLAKNIG
                     IEGMEKKLGKLFFDDLFNLYSVEDKYELFNVIRNNTQSTFLNLRTKVDIDEFSSGEKQ
                     IYVLCLYWALLKSSGIEIPFIIDTPYARIDDTHRNSITSEYFSTISHQIIILSTNTEI
                     DEKLYKIIKPKLSREYLLEYDSKNKKTIQNKGYFFEV"
     gene            complement(17607..19073)
                     /gene="dndC"
                     /locus_tag="OUB02_001104"
     CDS             complement(17607..19073)
                     /gene="dndC"
                     /locus_tag="OUB02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HCT8884877.1"
                     /translation="MAVNYLDNNNLENLYDEMQAVYLNDDRPWIIGYSSGKDSSCVVE
                     LTFRMLKRLPKEKRHKEIYVISSDTLIENPIILDYLKNNIDKINESATEHDLPISAQI
                     VYPELNDSFWANIIGRGYPTPKSIKYRWCTERLKIKPSNKFIKEKLEQKDVIVLLGVR
                     KEESSARKSRIEKREIEGYLLTPHETLRGKNKLAYVYTPIVNFSTADVWDVLYHQNDN
                     FIDFGGEFGPSPSTSWGTDAMELFQMYMKGSGDSDECPFIADEASAKSSCGNSRFGCW
                     ICTVVKEDKSLNGFIESGHDELKPLLEFRSWLISERDKPKNRKKHKRNGSVIKKDGKI
                     MFGPFTFEARQTILRKLLETQLEMQKFYPDLQLIQLGELKAIDEIWDNEEDLTGNILS
                     KIYKEVVGKNLPWSEYKKPVFDDLTLNIISEKCSEYDINIDLFNKLIIDTNKYKYFSN
                     NTKLKTSIERILNQQWLHQDIIEKIEEEGNKELKYENK"
     gene            19682..19834
                     /locus_tag="OUB02_001105"
     CDS             19682..19834
                     /locus_tag="OUB02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884878.1"
                     /translation="MKNLNKNKILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            20151..20396
                     /locus_tag="OUB02_001106"
     CDS             20151..20396
                     /locus_tag="OUB02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HCT8884879.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYAEN
                     IKSNTAILDSQEEVPISRYRYKDIKEGFLKFIGDRIC"
     gene            20390..21783
                     /locus_tag="OUB02_001107"
                     /pseudo
     CDS             20390..21783
                     /locus_tag="OUB02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            21930..22070
                     /locus_tag="OUB02_001108"
     CDS             21930..22070
                     /locus_tag="OUB02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884880.1"
                     /translation="MVIAISLTLGLITGCTNKISDTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            22043..22300
                     /locus_tag="OUB02_001109"
     CDS             22043..22300
                     /locus_tag="OUB02_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884881.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKDKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            22305..22484
                     /locus_tag="OUB02_001110"
     CDS             22305..22484
                     /locus_tag="OUB02_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884882.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEENVEVDNLGDIKIQVKKFDDK
                     TKPLELKLIKEFKLK"
     gene            22496..22690
                     /locus_tag="OUB02_001111"
     CDS             22496..22690
                     /locus_tag="OUB02_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCT8884883.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSSDDIERITKEKYTKEN"
     gene            22924..25476
                     /locus_tag="OUB02_001112"
     CDS             22924..25476
                     /locus_tag="OUB02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8884884.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPNDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKSDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDYIDEKLNDFVEEGSLDD
                     IKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMKV
                     RVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNNE
                     IQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNLL
                     IRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAYI
                     NYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            25493..26155
                     /locus_tag="OUB02_001113"
     CDS             25493..26155
                     /locus_tag="OUB02_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884885.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
BASE COUNT        10468 a         3033 c         3848 g         9851 t
ORIGIN      
        1 atagaagata gaagatagaa gatagaagat agaagataga agatagaaga tttataatat
       61 gtaatatttt tgttttgaaa tttataaatt agtattactg gttagagtta gtttagtatt
      121 aagtatattc gaagtagttt tttagctatt ttcatacatt tatattactt aattatatat
      181 agcctaacta acttaatata tcagtgaaat attgactaaa atgcctaaat atgttatcat
      241 acaagttagt caaataattt ggcaacatta aatatttagt aaatttctaa tatttatgga
      301 agaaatttct agcaaagaag gtgagattta tatggctggt aaaaaaatat tagcaacaaa
      361 caaaagagca agacatgaat attttataga agaaacttat gaatgtggaa ttgaattaaa
      421 aggaacagaa gtaaaatcaa taagacaagg caaagttaac ctaagtgatg gatttgcatc
      481 tgtagataat agcgaagtat ttttaaaaca agttcatata agtccatatg agcaaggaaa
      541 tatattcaat gttgattctt taagagttag aaaattatta cttcataagc atgaaataag
      601 aaagcttatt ggagcaacta ctataaaggg gtattcactt ataccactta gtttgtatct
      661 aaaaaacggt aaagttaaag tagagattgc cttagcaaaa ggtaaaaaac tatatgataa
      721 acgtcaagat atggctaaga aagatgcaca aagacgtata gaaagagaaa tgacaggtaa
      781 gtattaaact agataaaact gtatttttta gaataaaatt ctttattatc tagaacataa
      841 cattgatttt tattcttaaa agtattatat tatatataca ttaaaaattt aataaaaatg
      901 gtgataaagt ttgcgaaaag tctactggac tataaactac tcaagggggc gtaaaggttt
      961 cgacgtgggt ttggaacttg aggctgcatg tcgtgttact ctgggtcacg taaaaactgg
     1021 ggaactttaa aataaacgca gacgataatt tcgcgatagc agcctagttg ctgctcgtcc
     1081 ctcttagtcc tcctgccgac taagactgga cgtcattatg cagggaacta ctttaggggt
     1141 gtctcgactt gaagtagatt aattgggact ggctaagcat agagcctgcc acttggcgct
     1201 atgtgaggtg agatattagt gaagttggct aagcatgtag atgcagcaag gaaaagattt
     1261 gcggacaggg gttcgactcc cctcgtctcc accaattttg aaaaaagaat gatgaaatac
     1321 ttggaaatac tagtctttat tgactagtat tttttatttt ggagataaaa tccaccaacc
     1381 gtaccccaac cacagaaaat gaggtatttt tgagagtata tgggggtgca tttgagtgta
     1441 tgaaaacatt tacttatcat tacattagag aggataggaa tttaatacat actttaggga
     1501 gctttatcag aagccttaaa tatgaactca gaagaagttt ataaaatact taaacataac
     1561 actgatattt caaatacctt gttatgaatc attacatttt taaagtaaaa gttacatagt
     1621 agaagattta atagaaccac ttaaataaaa ggaagtgtta tgatgattgc atatcatagt
     1681 tcttattgta tagcaaaaac ttctgattct atattctaaa gaggttcttt aaattttgat
     1741 aaaagatttt atgtgattag tataaaaacg cagatgatta acaggatgga atgatttaag
     1801 agaagaggta aaaaggtgta tgtagatagt tgttttttag aattgatata aaatcaattt
     1861 aattatttaa tatttatttt tgtaaaatta aaagaactgt agatacgttt aaataaacca
     1921 ctatttcaca cgcgttttct aaaaatagtg gttaaaatgt tattttttat atttatttac
     1981 aactttagta atttgattat gatgaaaagg tttgttagga ttcaattttt tagttaatct
     2041 attagctatt tctctaagac taatgttaca atctaacata gataatattt cagtaatatt
     2101 ttcttctagt aattttatag aactttttgg aggtattaaa ttttgttttg atatatgttc
     2161 tttatatact tgcccaatca catttaaatc aaagggtttt cctattaaat tagaacctaa
     2221 tttatgtgat atttgtctta atgaataatt ataattatgt ttaaggtcaa taagagtatc
     2281 tatatgttta tttagtaatt ctctttgtgt aggaggtttt tctttaagtt cttctcgacg
     2341 agtatatttg taatttttat aaatacttat tactgtttta tggtcataag gtttatctct
     2401 tttcaccaaa atagcaattt ccctaccaat tttactaaat gagtaatttt ccacatctct
     2461 taattgtaca atctttttta tgatttcttg attatacaaa taataatctc ggttagttct
     2521 tctacgctga actttatcat tcatattatc tttttggtcg ccagttctta agtgacttgc
     2581 tctaatacat aatggattat cacaattgtg tagaatatat ttgccactac ttgaagtatt
     2641 agtataagtg tcataagagt acctacttgc ttttatttgt ttgttatttt tatggactac
     2701 aggacagtat ttttcacttc cccaatttgc ttcaatacag acttctttag ggaaaaaaat
     2761 ttcaagttta tgtaatgcct ctactttttc tgcattagtt aaataaatat aattccatac
     2821 actacctttt ttaatataga gtaatagctc taatggtata gataaaaatt tagctatttc
     2881 ttctattgaa taattaaatt cagagttatt catattattt aataaatatt ttgttaaata
     2941 tactgattct ttagttaaaa ctatctcatt aatttctggt ctatcattga aatatttatc
     3001 aagaatattt tcagatactt tacgttcttc tatatgactt ggattaacac aacatcgatt
     3061 gtaacaagta tgatgtgctt ggtatccatt tctaattggc ctttgtagtc tctcttctag
     3121 tactattctt gcagctctta aagtaccttc tttatttgaa ttaatttgac cataaccatc
     3181 acggtctcta tatcctggat aaggaatgca ttcaatcaat tttatcacct catttattaa
     3241 tattaaatta atttgtatca ttattttgat tttgtaatta actatatgaa atcatagtat
     3301 ttttatgtgt ttgattttta caatgaaaat attttatcta acttttctac agcttcaact
     3361 ttcttatctt ccataacttg agtatatata tttaaagtta ttgatatatc ataatgaccc
     3421 attaaaactt gtacagcttt aggagggaca ttattctcaa aaagtctagt cgcataagtt
     3481 ttcctaagac catgaaactt tataggctct atatctagac tgtttaatat agattttaga
     3541 ttccttaaag gtcttttatc atctataggg acacctaatt tattgcaaaa tacataatta
     3601 tcatttctat ataattctcc agcctttaat atatctgtat tttgacttac tttatgttct
     3661 ttcaatttat ttaagatatt ctttggaata ggtacagttc tgtaagaatt tgatgtcttt
     3721 ggctcttgct ctaatacttt aagctctcta tctccatttt tatctataaa atatgttctt
     3781 tgaagcgttc ttttaactgt taaattagaa ttcttaaaat taatgtcaaa ccatttaagt
     3841 cctaataatt ctcctaatct aagtccagta ccaagagcaa gtaaaaataa tacctctaat
     3901 ttatgtccat ctatagcttt tataaaactc ttttgttgtt caagagtcaa tatttcaatc
     3961 ttattttcct ttttctcaac tggtatattt accatagtac aataattacg ttgtataaga
     4021 ccttgttttt cagcttctga taagcaggtt ttcagtttag tgtttatttg ccttatagta
     4081 ggtatacttt tattggaatc aagcaatcta ttgtaatatt tttgtatgtg agatactctt
     4141 aagtcattta atttaacatt gactaaatca gtatctttaa tataattgcg atagatgctt
     4201 tcatatctct caaatgactt aggttttaaa tcttttattc tatatttaaa taaccaattg
     4261 taaaaccaat gctctaaagt tattttatca tctgaaatat cagagcttaa ataatattgt
     4321 tttttataag catctaattt atctttaact tctttttgag tcttaccata aaattctttt
     4381 cttattggtt tcccatcttc attgtaacca atcattatac gggaacgcca accatcttta
     4441 tattttccga tagagccttc tccattagct attttagttg ccatttttaa acagctcctt
     4501 atatgattta tagttatatt catcaaagaa tgtaatacat ttctctaata agaattttat
     4561 aacttcatca ccttctagtg tttcatagaa gtttagcatt aatgtgttgt attcttgttt
     4621 atgtttagct ggtattgata gaactcctat atcattactt ataagtatta ggtttgaggt
     4681 aagtctagca gttcttttgt tgccatcata gaagaattga caataggaca accataaatt
     4741 tattattata gccttttcta aggggttagt tattttattt atgatattta tgtcatttac
     4801 aaataaatca tttaatagag tgggttctat gcactcatat tgggtagtac cagctatacc
     4861 tatattacca tttctgaaat ctccaacgat tagagcttca tcttttgcaa ctattttatg
     4921 tatagagcaa attgtatctt tatttaaaga taatttttct ttgttactta attttattac
     4981 ataatcccat gatgatttta tatttagtat ttgattttgg tcacttaatt tatgtccacc
     5041 tactgttata ccatcaagta atgtttgtac ttctgggaat gtgaaggggt tattttctag
     5101 gttacccata tcatatacaa attcacttat cattcttcta atgtatgtta aagtatattt
     5161 tttatctcca tgaggtagat tagtagggaa tttatgtgtg tattttggta tatatgattt
     5221 attcatgatt ttagctcctt tcatatctaa tttttattta ttaactatga atgtataaaa
     5281 gttaataggt aaacctatat tttgaaaata tgcaatacta atattcttca tcctcatctt
     5341 caataagacc attagagttt tcttgtataa aattttcaac aagaccattt atcacccaat
     5401 ttagattttt ggagaagtct ttgaagtcag agaagtttag gttaattggt tttattgtat
     5461 ctgcattatc tgtaatttca aacttttttt atagtctgtt tttatatcat aaccataatt
     5521 ttctaatatg tgaattacta aatccaactc tttatcaaat acttttaaat taatattatt
     5581 atttgttaaa gtatctaaat cattttcttc aacaatgagt ttatttatag caattgataa
     5641 agcagtagct atttttctta tcatatctaa actaggtatt ctttgatttt gctcatattt
     5701 agctatagca tgaactgaac aaccaatctt gttggttagt tcattccttg atatatttat
     5761 cgatttccta tattgtttaa tcttttctcc aattgtatta tttattataa ttccacctta
     5821 aatatatcat caaacaatga ttatattttt acacatataa aggagttatt ttctagatta
     5881 tctatgttat atactaactt actcataatt cttttaatgt atatcagagt atatttttta
     5941 tctccatgaa ataaattagt agggcattta tatgtgtatt ttagcatata tgatttattc
     6001 ataattgtaa ctccattcat agttttttat cattgcttta gatagtattg atattatttt
     6061 atattttagt tagtagtttt tttatttctt taagtgtatt ttcaagcttt tcattttttt
     6121 cctttaattc gttattttct tgtaataatt taaagtaatc attattttta atagtaatat
     6181 tttctttttg agatattaat ttttttagtc gaatattact tccattatct gttgagtcac
     6241 taaagtaatc atagattgtt tctatatttt cttctgatag accaatatct aataattcat
     6301 caattgattg ttttgtaaga acatcatttt taataatttc ttttatgtca tcaggatgta
     6361 atgaaaaatc taattcagta ttagatatac aatctgaaag tatccaatta taaacttgat
     6421 tttcatttag ttctaatttt tcaccaagtt tataatattg gtctttatca gaccaagcac
     6481 ctagcaataa acttgaggta attctaatat catcatcttc cataagattt gaaatagcat
     6541 ttaaaaattc atcaaaagaa tagtattctt ctgggaataa agactgtaaa aagtataaaa
     6601 catcttcatt agtaattact tcattttcaa tatcaaaata aaagatagtc ttttcaattt
     6661 tcaaagcttt agcgagtttt gataaggtat ctatattagg tattctttta tcattttcat
     6721 aatttattag agcatttctt gagatgtcac taatttcagc aagttgaatc tgtgttagtt
     6781 ctttttgttt tcttatcttt tttaagtttt ttccaatttg caatattatc acctcgtata
     6841 aagtatatcc taaaagtaca caaatgagaa cagaagaata atatattttt attgggtata
     6901 tacatatatg gacattataa ttaaaatata aagtttacaa atgtaaacta gaaaaggtga
     6961 taatactctt atatatcagt tagaaaatga tataaataaa actaatcaga aattaataaa
     7021 aaaggattca agtataacgg ctatatcaga gagagcattt gaataatata aatgatgaaa
     7081 taaatgaagg tgctaagact agaataaagt tcctagagaa tattatagga ggtttatatg
     7141 gtggtgaact aactccaata gcagttaaaa gtagaagagg aaaaatagca ttagctttac
     7201 aaatacttag aaatgtaata cttcaaggta agaaagtatt ttttataagt agagaaatga
     7261 gtaatataca gattttgttt agaaatatag catcactaac tggaatatca attgtaaaaa
     7321 tgaagaataa aaacttagat gatgatgaaa tagtcaaata tatacaagtt ttaagtataa
     7381 taaatcaaga aaataatctt tatataaatg actctgtaaa tacaatatca gggataaagc
     7441 gagaaataaa gagtattaaa tctagtttag taataattga ttatttgcaa attttggatt
     7501 atgaagctaa agaaatagga gaaactaaat ttgctgactt atcagagcaa gtaaaaaata
     7561 ttactttaaa atttaatata tcagttgttc aatttgctca attaagtaca tatgcataga
     7621 gatattttag aaaaagataa gttatgtagg atattgcaaa gtgattagag agattaataa
     7681 attgttagaa aaggttttac tagatgggaa taaaaaatgt gtattagtag agtgggtatt
     7741 agatagtatc attttagaag ttatatatag catgtttata ttaaagtagt aaatatatta
     7801 atctatgtac ttgtttttta agtaaaataa tagcataaaa taaaggtttt taaaagtata
     7861 tgtctaattt tataaataaa catattatat aaggacctga ataaatattt gaagagataa
     7921 aattatatta aacttaatat caaaatgctt aaagatgtct tattttaata ttccttatca
     7981 tagttagaac ttatagaatt ctatatatca taatagtggt aaatttaaat atgtatagaa
     8041 gattaaataa taatatatgt ttattataaa aaataataca aattaatgtg tataaaaaga
     8101 tttactaaat aagtataatc aagtaaaaat taaatatgaa gatattaatt atactagaaa
     8161 gataatagta taagcaatta aggatgcaat attaaattct taataaactt atatctaggg
     8221 aggatatcaa atgggaaaga ttgaacctaa tcaagaggac cgtatatatg aagatttata
     8281 ccagggaaat agcgctgaag gtaaggtatg tgaatggata gaagaagcta ttaatgatga
     8341 ttgctatgta ggacaacata gatttaagat tgagacaaga gaatgtgata ttgctatggt
     8401 tataccaaat agaggcatat taataataga agtaaagggc tatactgtag aaatgattga
     8461 tcatatgtca agtaatttta ttatacatgt aaagaaagag tatgaaaatg aaagatttaa
     8521 aactggtgaa aggcatccag cagaacaagc aaatgattat gtatttacaa tgatgagaga
     8581 tttttcaggt tttttaaaaa ggaataacat atgtttacca atatgtaaaa taggacgaat
     8641 ggcagcaatg cctaatatga cagataaaga gtatgaggaa ttatacttgg attattgtta
     8701 ttcaacaaaa cttgtaatca caaaagattg gtttaagaat aaaaatatat tttaccatcg
     8761 ttttataaat gcacttaata taatttttaa ttctataagc agtgaagtag atttcgatga
     8821 tagttattat aatattgcga gaaggtatat tcttttaggt gaacgtaaaa aatatgtaga
     8881 tctatgtaaa gagcaactta agcgagaaga agaattgggt attgatttta taatagacaa
     8941 tactaatgaa gaagtagatc aactttgcaa atcgaatgaa gttaatgatg aagaaatgga
     9001 attgataaaa ttttcttctg ttttacaaga agaattaacg gaatcacata aactatctaa
     9061 aattattcct tattctaaat tacgtatagt taagacaggt tgtgaaatag aaccactttt
     9121 ggagatttgt tgtaatgaac gtaaagaagg aataaagcag tttctaatat tagttaattt
     9181 tacaagagat gagaaagaaa aaatattgtt agaatatgaa aaaagatttt taaataagta
     9241 taaggaaaat gaactgttat ttttattcaa tatgtatttt atagatttat ctaatgatga
     9301 tacaagcatt gacaatctat ttagtaatca tgataatatt gaaattatta atggagagag
     9361 aatagaagag attgtcaaaa taaaacctga caattttatt gtacaagaat ttacaccaaa
     9421 agatctgtta gatcaagctt tgtggaatat attatgtatt gcagataatt ttacttcatt
     9481 taattttgat cagtatatag ttgaacattc acctcttcgt caaaacattg ttgtaactgc
     9541 aagtgctggt acaggaaaga catatactat ggttgatcga gtttcatttg ttgcacatat
     9601 ggagattatt tatagaaact ctaaggaatc tattagtgat ctaatctcta tgataacatt
     9661 tactaataat tcaacagatg agatgaagga acgattaatg aaacatttta tgcagtactt
     9721 tgagttaaca cataataaag tatatatgga tttattagaa caattaggta gaatgaaaat
     9781 taaaacaata gattcattta caaaatatat tttatctaaa tttgctcatt tattaggtct
     9841 ttctaatagt ttctcattaa aaagcggaaa gtttgaatta gagaatattt taagaaaata
     9901 tattaatgat gagataacta aggaaattat agatgatttc cataatataa atatttatga
     9961 aattgctaat catattcttg ctttgcgttc gtttcttatt aatcgaaaga ttcaatttga
    10021 taaaataaaa catgcttcta ttgtgggacc tgatgtaggg aagggaacta ttcgtatgaa
    10081 aaattttata gagtctgtac ttatttctac aaatagtgaa tatgatagtt ttaattgtag
    10141 tcaaaacttt atttcgatga atgaattagt tattcaatta gagaaggtca aaaataaatt
    10201 gataaaaact agttgtgtta ataatactaa agaaaattat gaaaaatact tgtttataga
    10261 tgaatttcaa gatacagata ataaacagat acaattaata agattttttg cggactattt
    10321 caatattgca ctttttgttg ttggagatga aaaacagtgt atttatggat tcagaggagc
    10381 agaaagaact tcatttttag aacttacatg tgtagatact aactttcaac aaagaaattg
    10441 gatatcatgt tttctaagaa aaaattatag aacagaggaa cccttagtaa aaaaaataaa
    10501 tgaagtgttt tctaaaatgc caaatgattt tttcccttat caaaataaag ataaattagt
    10561 agctcaaaaa ggaattggaa gaatttattc agggtgttat gaacaattta aatatagtga
    10621 aaataactta atagaactcg tagataattg tgttaaaaga atgaaaaaaa ataatgaaga
    10681 tggacaaatt gctattttaa caagaaataa ttatgaggtt gatcaaatac gtgctttgtt
    10741 tggagagaat aaagaatatg aaattaaatt tgataatggt ggattatttt ataatactga
    10801 tgctgttatt gatttttata aattagttga gtttttgatg gatacagaag atggtgctaa
    10861 acgatatagt attttagaaa ctccttatag tgatttcaga attaaagaat cgaaaataaa
    10921 gctatatcaa gcttatgaaa aaatggatgt tgaaaccttt gaaataatta ggtctggaaa
    10981 taatcatgta tctttacgaa aatattatga tgtattatat aattatggga aaaaacatcc
    11041 aaatttaaag aatagcccaa gtttaaagat ccttcgaatg attgttaatg aaattaaacc
    11101 atgggaacac tatgtaccag aaagatataa aactgttgat tttaatgata tatgtaacca
    11161 gaataagctc attgaagcta gaaatgagta tcgtcaaaat ttatttcagt tatttcaaaa
    11221 attaatagat gataataaaa cagactattt aacacttaat aagatacatc aaattttaac
    11281 tattggtatt tttgcaatgc aagatattga tattgatttg cctaaaaata atttttttgg
    11341 agaaaaaata agtgtattat gtacaacagt acataaatct aaaggtcttc agtatcatac
    11401 agtaatcatg ccatttacaa tgaatgatat aacagagtca agaggatcaa cacaatttat
    11461 ttatagagaa gatacaaatg agatctatat gcaattaaaa gaagctgatg gaaagtgtct
    11521 aatttatgaa tcaaccaatt ttgattgtga aaataataag aaaaatgaaa ctatacaatc
    11581 agaagaatta agaattttat atgttgcatt aacacgttca aaaaaatttt tttactttat
    11641 ggtggatgaa gaaagatttg aaaaaaataa tacttctaaa aaaaattgtt gggcacatta
    11701 tctaatgtaa ggtggaggaa aataatatga aaatctatac atataaaaat tctgtacaat
    11761 tgaaagatgc aatcttctgg aatgaaatag aaggagcacc tcatttttgt tcctctgaag
    11821 tactagcaat aggaatgaga aaattttatg gtagaaatac ttttccatgc atttctacta
    11881 tagatttatt gataaaagaa ctttatccaa attggcttaa atctcatagt gatatagagc
    11941 gatataaaga tatatctaaa ttaattaatg aacttaatga ttcaagtaca gagggtttaa
    12001 gacaagcttt tgtaaaaaat aaaaaatcta tactagattc ttgtaaatat ttgattgaaa
    12061 ctgaagattt gctcttaaaa gattattaca ataagaacat atttaataaa ttttcagcaa
    12121 ttaatgatat tcaagaagtt tttaaagaaa agatactgaa aagactttta gaacaaatat
    12181 acattgatgg aaacaaaaat tattggaaac ctgagtggat agatacatta tgggttaaat
    12241 caaagttaag tgaagtaatt cataaatgtg tgataaaaga aattagtgat attgttaaga
    12301 atacagaaga aaagagttta aatcataatt atgatgaagg tcatattttt gaaatatttg
    12361 atgaactgtt tgaccagcat ggattattta atatatcaga taagacttta gaatattgga
    12421 aatttaattc taaagatgat ttgcttgata aaattggata ctcatcaaaa gaactagagt
    12481 atgttcgtaa tctagctact actgaatgta agtcatataa gactatagta attcatggtg
    12541 tctatcattt aaatccaatt cacttaagat tgttaagaga tttagaaaga gatcataatg
    12601 taattgtatt aaattgttat aataaagaat tcaatcatat ttatgaattt tggaatgata
    12661 tatatgatca tctaattaaa atatttcaaa ttccaaagaa taatataaaa attgatagtc
    12721 atgattctgc taatcacaga gaagttggta gtatatttgc tgaagttgta aatggtgtaa
    12781 tcaataaaca aagtaatttc tttaagataa gcagaaatga atttggccca gacttaaatt
    12841 taaattctaa ggatattgat gaacttttta atcataatag tagtcaggat attagatata
    12901 tattaaataa aagtgaagct attattcaaa actatgagga tgaagatgaa agagagtatc
    12961 agaagatact tatagataca tcaaaagaac ttcaaaaaga actatgtcat agaagtaaga
    13021 ttttaataaa atatgattct acaatgcaat ttgtcaataa agtgtgtaat atatttgatg
    13081 aaacaaagaa taaaaaaggg atacgttcag tcggaaggat gaaggagcaa ttttatggtt
    13141 ctaaaggtac agaaatgaat aaaatatttc aagtatttta tccagatgaa tttaaagtta
    13201 agccttttct tagttatcca attggacaat ttatttatgc aattcataat atgtgggatt
    13261 tgaagaatca taaacttata ttaaatagta gagatttagc tgaatgttta aatttagatg
    13321 tatggcatga tattcatcca ttagaggtat ttgaaagttt acgtttttat attggtattg
    13381 ataaagagcc taatggtatg aatgtaaaag attttattga tcgaataaaa aaactaatat
    13441 attttaaaga aagaaacgaa aataaaatga agaatttaga taatcttagt tatcttaatt
    13501 taactataga ccaatataat agatttattc aagtaattga aaacattaac gaaattgcaa
    13561 ataagatatt ttgtaataaa tcacaaacaa gtaaaaagca ttttatggat ttactagaaa
    13621 tattaaatga tgaaaaagct tggaaacctc tattagaaga agaaaaaaga ttaatggatg
    13681 aaattaaaaa tcaaattatt catattgatg atgaaactaa taagactgac ttaaacacaa
    13741 taaaggatac tatggcatat tatttagata gcaaagaagg tactgatatt aattggttgg
    13801 tgcgtgattt tgagcaatta gaaggcgata ttctttcaga agcagcaaat tataaaaata
    13861 atactttgag acaaaaatgc taccatttaa ctatgttgtc aaatgaaaat atgattaatt
    13921 gtgatataga taatttacca tggccattaa gtgatgattt tattatatca aaaaatattt
    13981 cttcctttat acatacaatt tgtataaact atgcaaaata taaacgttgt ctattgtttc
    14041 aaggattata ttatttgata aataatccaa ttatttcact taagttaagt tatattgaaa
    14101 atcaaggaga gtataaacat acaccatact atattttaaa catgtttatg aatataggaa
    14161 aggattttga tcaatcaaat tgcagaaata ttgaaagttg cacttctcca attaataaag
    14221 agccaaaaaa acgttttaaa atagataaaa aatggcataa agatgctata gagtcattta
    14281 gttgttgccc atataagttt atgtatggat atgttattaa tgatgctgca aaacattata
    14341 aagatgagtt acaagtgaag atgtatttag gagaaattat aaaatattat attagtattg
    14401 cgtggaaaaa aattttacaa taccataaaa aactattatt ggatcaaaag tttaatttac
    14461 tcaaaaaatg gactgctggt gaaagactag attgtcttga aaaatcttta ccagagacac
    14521 tagaacctat ttatggtaat tatttcatca aatctgagat taataaaatt atagtagata
    14581 acttagaata tttaaaaaat aaaagaattg ataataaaag agtatatttt acagaggagt
    14641 gtaaaatata tgaagttgat agagaactat atcattgttg gataggtaaa atatcaaatc
    14701 ataatttata tgtaacaaat aaaaataaaa taattaatat tagaaatcgt ctaaacttac
    14761 taaatgcaaa tgaatatata aatgactata ttcttggaat taatgatgga tggtatttta
    14821 aaaaagataa tattgtagtt gagggaggat ataaagtatc tgaacatgct tgtagatatt
    14881 gctcatataa taaaatatgt tgttatcagt ataggataaa acctatacac ttgctacatt
    14941 caaaagaaga tttaattgat taataattag ataaacttta agaactatat caataggata
    15001 gacggtttta tttattttct atcctattga ttaaatctaa aagttttagt tttttagtta
    15061 catattgaat catattatag attgttatct tcttgtatta tttgagtgat aaaatgttct
    15121 aaattactgg aataattgta tctgttttta agcataattg cacccctatc tatatgaagc
    15181 tttacttggt taggaaaata ttcattatca cttaaatgtc tacctatatg ctgttcaaat
    15241 aaagttttaa ataaagtatc atattgattg gttatggttt gtctattaag ctcaagacca
    15301 ttattatcaa tgttctcata actttcaatt gattcactat aacttaacga cattgaaata
    15361 gccagtttgc aaagagcaaa tggtttcaaa ttcattctag atcctaactc ttcaaatatt
    15421 tcttttgttt gtgttgatgt tttaagttta aatctcataa ttacacctca aaaaagtatc
    15481 ctttattttg tatagttttt ttgtttttgc tatcatattc aagcaagtat tctctactta
    15541 acttaggttt tattatttta tataatttct catctatttc agtattagta gatagtatta
    15601 taatttgatg acttattgta gaaaaatatt cactagtaat gctatttcta tgagtatcat
    15661 ctattctagc ataaggagtg tctataataa aggggatttc tatacctgag gatttaagta
    15721 aagcccaata taaacaaaga acatatattt gtttctcgcc actagagaat tcatctatgt
    15781 caactttagt tcttagattt aaaaatgtag attgagtatt atttcttata acattaaata
    15841 gctcatattt atcttctacg ctatacaaat taaataaatc atcaaaaaat aattttccca
    15901 actttttctc cattccttca attcctatgt tttttgctaa attttcaagc tctaaagagt
    15961 tatatatttt atttatatat aaagaaatat caaagttgac attaatatct atgtggctaa
    16021 taaaattgtc tttttgaatt atctttttaa atatatacat aaagttatcc tgaattagtt
    16081 taattttagt ttctgttaat gttgttacta tttcgtccaa cataagtata agattagtag
    16141 atatatcaac aatattatta tgttgaagta aatatatgta tttagcctta gatttatccc
    16201 tcctactttt agtttcagct atttcaagtt ttaatttatc taaagaagta actagtaaat
    16261 tctttgatcc taataaagaa tcaattttgc ttgataaatt ggacatatca ttaatatatg
    16321 tatttaaaga ttcatcttta aggctagtat ttaacttgtg tcttatatca gctaattcat
    16381 ttgatatact tctatgttct tcaaaagaat taattataga attattatca aaatttaaaa
    16441 tagtgtttat cagagataat aattttctac tctcatcatc agaaaggtta tggatagatt
    16501 taaagttttc ttcataacta tctggcttta tatcttctgt taaagcttca gaaacagtta
    16561 aagctatttc atctaaggca gaaggcatga tcttatctga aagtagattt tttatatact
    16621 caatatttaa tttatcctta aggcgagaat atagtaggat atcattctct gttttaagtt
    16681 gtttttctaa atttgatatc tgttttttta caattaaaaa gggaagtata tcattacaaa
    16741 actcttttat attttgatta attataaatc tctttccttc taattcagca attctagaat
    16801 taagggtttc tctttcttct gctaggattc cacccttttt cctgaattct ttttttactt
    16861 ctaattgttc ttgttgtaac ttctcaattt catttgaaat tttttgtaat tcataagaaa
    16921 aaatttcttc ttgagagttt aaagtattga tttccatctc taaatttaaa tagctatctt
    16981 ttacaatatt aatttcatct gaaacattct tattagatct attagttgaa atcaacactt
    17041 tttttaaaat atcaaatgta tcaaaattac aaagagataa taaagattct tttaaatgta
    17101 tatttgaatt tttacctaca aaaaaatcat acaaatgttc tccatcaaaa aagaaaaaat
    17161 caaagatttt aggagaaata atagatgtaa tataattctc aaaatagtta agttcgtcag
    17221 catttaatgg tgtagttgaa aaatttttat aaactaaaaa tgtctcattt aatttttcat
    17281 ccttgaaagt ccattgtcta attaaagtat agatattttc ttcagtgcct tcactgattt
    17341 ctatatctac tgatactaaa gtatcaatct tactatttat tagagaatta ttgtttatgt
    17401 tagatttaac ttgagatata tatgaagcgt tgaaaccttg atatttataa gccataggtc
    17461 catatataca tagctttata gcctcaaata ttgtagattt accagcacca tttttaccac
    17521 ctatcaatat aatatttttg tcactagtag tatttagatt gaatgttgtt tcttcttcat
    17581 aagatctgaa attttttaat acaagtttat ttattttcat attttaactc cttgttgcct
    17641 tcttcttcaa tcttttcaat aatatcttga tgaagccact gttgatttaa tattctttct
    17701 atggaagttt ttaatttagt attattacta aaatatttat atttatttgt atctatgatt
    17761 aatttattaa ataaatcaat atttatatca tactcactac atttttcaga tataatattt
    17821 aaagtaaggt catcaaatac tggtttttta tattcagacc atggtaggtt tttacctaca
    17881 acttccttat atatttttga taatatatta ccagttaagt cttcttcgtt atcccaaatc
    17941 tcatctatag ctttaagttc tcctaattgt ataagttgta aatcaggata aaatttttgc
    18001 atttcaagtt gagtttctaa aagttttctt aaaatagttt gccttgcttc aaatgtaaat
    18061 ggtccaaaca tgattttacc atctttttta ataacagagc catttctttt atgtttttta
    18121 cgatttttag gtttatctct ctcagaaatt agccaacttc tgaattccaa aagtggcttt
    18181 aattcatcat gaccagattc aataaatccg tttaatgatt tgtcttcttt tacaacagta
    18241 caaatccaac atccaaacct actatttcca cagctacttt tagcactagc ttcatctgct
    18301 ataaatgggc attcatctga gtcaccagaa cctttcatat acatctgaaa taattccata
    18361 gcatcagttc cccatgaggt agatggagat ggtccaaatt caccaccaaa atctatgaaa
    18421 ttatcatttt gatgatagag tacatcccaa acatctgctg tactaaaatt gactataggg
    18481 gtatacacat aagctaactt atttttacct cttaaggttt catgtggggt aagaaggtaa
    18541 ccctctatct ctcttttttc gattctcgat tttctagcag aactttcttc tttacgaacg
    18601 ccaagcagta caataacatc tttttgttca agtttttctt ttataaattt atttgatggt
    18661 tttatcttaa gcctttcagt acaccaacga tatttaatag actttggagt agggtaacct
    18721 ctaccaatta tattggccca aaaactatca ttaagttctg ggtatacaat ctgtgcactt
    18781 ataggtaaat catgttcagt agcactttca tttatcttgt ctatattatt ttttagataa
    18841 tcaagtatta ttggattttc tataagtgta tcagatgaga ttacataaat ctctttatgt
    18901 cttttttctt tgggtagtct tttaagcatt ctaaatgtaa gttcaactac acaagaacta
    18961 tcttttccag aactatatcc gattatccaa ggtctatcat cattaagata tacggcctgc
    19021 atttcatcat ataaattttc taaattgttg ttatctaggt aattaacagc catatattaa
    19081 gtatcctttc tataagagta tagcgaagat ctttacataa tactcctatt ttaatttata
    19141 tataattata tcacaatata tatttagaga atagattagt ttatatttac ataagtacaa
    19201 taaaatataa atttaattaa atgtagaact gttatcattt taagataata tatttaaaaa
    19261 ttagagaata agaatgtaca tacttgaatc tcataaatgt atataaagat atgtatgaat
    19321 aaaaatagca gctacttaga atagttacac tatttaagat ttattatatt atagattgac
    19381 taaacaatag atatttatta atgactcaat ttataaactt taaaagttat atcccaataa
    19441 taccccaacc acaaaaataa acaataaaaa ataaccaaaa aaatcagtct aatatcataa
    19501 ctaaatagct atatacctac attcacaaaa accaacaaaa caatcaaata accacttata
    19561 gactcccctc gtctccacca ttgaaatcca cgaataccta tatccaattg gatataggta
    19621 tttttataca tttttagttt aaatattatt gataaataca tttaaaaagt tttaacctaa
    19681 agtgaagaat ctaaataaaa ataaaatatt aggagaggtt atcattacaa taacagcttg
    19741 tgatgatgaa aaagaaatac aaatcgtaat aaaaaatcaa gttgataata tattaaaaaa
    19801 taaaaacaaa gaatatcgaa ttaaaaaaat atagctcaaa agaagaacca ttacaaagtt
    19861 aattgagagg atacactact cttagatata aaaatgccag aactaaatag tatagatatc
    19921 gcaagaaaca taagaaaagt agataataat atttataaca tctctaataa attatgtaca
    19981 ggagagatat gaagtaagag attattgata cttattaaag ccaataaatt ttcaagaact
    20041 agaaaaacac atattaacat atatcaaaaa aattgaaaaa aataaaaata agtctaacac
    20101 atacaaaatc tactctagca aaaaaataga tagaagttca aaaaaagaac atgctaatac
    20161 atacaaaaaa taaaactttt gatgctatat gtatcctaga aaaaatagaa aaagatttaa
    20221 atatagagga acttgtaaga tgtcataaaa gttatataat taacttaaat tatgcagaaa
    20281 atataaagtc aaatactgca atattagata gtcaagaaga agtacctatt agtagatata
    20341 gatataaaga tataaaagag gggtttctaa aatttattgg tgatagaata tgctaaacca
    20401 tataaataaa atattattaa tattatcatt gtttacacca ataatgatac taaaaagtat
    20461 attaacttac gataaagata cagataagac taagaaaata tcttttttta ctgtattatt
    20521 agcttatctt ataactaaaa tcattatatt atttaacgtt gataagaccc ataattataa
    20581 tatgaagaaa aatctatatg atataatcta ttatttgata tttattattt gggaacttac
    20641 tataattatt tttataagat aggaattgtt aaatatatag tttcattttt cttatctgac
    20701 gtatatcacc ctttaataaa agatgttata tctataaatt tattttttgt agttacaagt
    20761 aatgtaatat catcagagga agctctatta gtgtttagtt atgataatgc taaagtagat
    20821 atatactatt atcttataat aatagttata tagtagcttg tgctatattt aaatttatta
    20881 ataaaagatt gggactagat aaagataata aaataaacta tatatactta tctgttgctt
    20941 taataacaaa tttatttact atattaataa tttatataaa taaaaaaatg tatcgatttg
    21001 gtgggtatta ggaaatatat atagttgggt atattgtgat acctaggtta atactagcta
    21061 gtagcttagt aattattata gtgtttataa aaataattaa ggaaaataag ttaaaattgc
    21121 aaaatgaagt aataaaaaat aagttagata tgcagtattc tcattatcta agtattcaag
    21181 aatcacacgc taaagttaag aagctttatc atgatataaa taatcatata tattgtatag
    21241 aaaatctcaa atataaaaaa ggaaaattga tgggtatgta aatagtttaa aagattaaat
    21301 taaagaattt aaatcaattt acaatacagg taatgttata ttggatataa taataaatga
    21361 aaaaagtgat gtaagtgaaa aaagaaaaat taaattcatt tgtgatatca atttttccaa
    21421 agtgaatttt ataaaacaaa tagatgtatc taatatattt gcaaatactt agataatgca
    21481 atagaagctt gcgataagat acatgataaa agtattgata aatatataca aataaaagga
    21541 actatctcaa agtcctattt cgtaattaaa tgtgagaata gtaaaataaa ttcaattaga
    21601 aagaataaaa gtttattatt aacagataaa aaagataaac ttgtacatgg aatagggatt
    21661 taaagcatca aatcttctat aaaaaaatat agaggagatt tatcatttga agataaaaaa
    21721 gacaaattta tattgaatat ttatatacca ttaaatcaaa attttgatag ttgggtcgat
    21781 taacctacca gttggggaac atatattgat actaaacata tagggatgtt atcttttaat
    21841 agaaaaagat agcgtctttt ttttatgaga aataacgagt tttcaatata taaggaggat
    21901 tatatgaaga taaaaaaatt acaataacta tggttatagc tataagctta acactaggtt
    21961 taataacagg atgcacaaat aagatttcag atacaaatga aaaaatatat actattgaac
    22021 agatttctag tatcaagaag acttgtcaaa gaaaatatca gaatcaatag aaaatagtgg
    22081 tcttagggta gctgaaaata aatcagatgg aagttttgtt ttgtctttgg gaaacttgga
    22141 gtatacaaaa gaacaaccta gccaagtttt acattattca ataactaagg acaagaagga
    22201 tacgaaggaa atattatcaa ttcaatgtgt taaagatttt tcttataatg aaaagctgtc
    22261 agaagatgat aaattcataa tggccattta taacatatag atcattgatt catatagaaa
    22321 taagtgagaa agaatttttt agaaaaattg aagaagtatt tgataaaggt gaagaaaatg
    22381 tagaggttga taatttggga gatataaaaa ttcaagttaa gaaatttgat gataaaacta
    22441 agcctttaga attaaaactt attaaagaat ttaaattgaa gtaggaagat tcactatgag
    22501 aaaatacaaa gctattttat taactttaat tataagcatt atagccataa taggatttat
    22561 tatatacaac aactatttta gaaaatggaa tgaagctgaa aaatatatag ataaatatat
    22621 gacatatcaa ggagtttcaa gtgatgatat agagcgtatt acaaaagaaa agtatacaaa
    22681 agagaattag gatgggatac tttataaatc attttacaaa aactatccag gttataaata
    22741 ctaatatttt tataatggtg attattatgc actacatgta aataaggtga tactgcaagt
    22801 ttatcatgag gaagatggta gtaagcttca aaaaataatg aagaattaaa agatttaaaa
    22861 tatccaccaa tctacttaac agatgagttg gataaataat ttatagtaaa ataggaggat
    22921 tatatgggga tttacttaaa aatgtcacta gcatatttaa agaaaaataa acttagaaca
    22981 ttacttttaa taataggagt tatgttaggt gttatgttga tatttggacc tagtgttata
    23041 aaagattcac aaaatgaaaa caatgtagta gcaatacatg aactttacag tggataccat
    23101 gtagagttta gtgatttgac acttgatgat gctaaaaaat tagaaactga tgaaaaagta
    23161 tcaaagggta attcggtaca aaatctagga cgtatttcag ataaaaaagg aaactcattt
    23221 atattaactt catcaaacga agagtatcta aaaagtagat acaacaaaat aatagaaggt
    23281 agactaccta aaaacaacaa tgaaatagta ttagagaaaa aagcactaga agcaatgaat
    23341 ttaagtgaag gagtaggatc tacattaaat ttaacaataa aaaagaacta taaagatact
    23401 aacggaagta atcaattatt ttctataaat aaagaattta aaatagtagg agttatggaa
    23461 aaaccaaaag ggtactatga tgtggtttgg caatacgaag cagtaactta tggcaatgat
    23521 gaaacaaaca acattatacc taatgatgcg attagttata atagtatatt aagttttaaa
    23581 agtggatgga gaaatattga aggtgaatgt gattatctta tgaaaaaata taagttagga
    23641 caaaaaagct ttctaccaaa tgtacctctt gtaagggaac ttcgttatgt agaagatgat
    23701 aagagtgatc cgaatgtttt taaaagaaat gttttaataa ctacagtatc tataattttc
    23761 atatttaata ttttcaatat tacactaaat caatcagtaa aagaaatggg actgtttaga
    23821 ctgatgggtg caaagaaaag aaacgtaagg tgtatgataa tctatcaagc attaattata
    23881 acaataatag gtatactact tgggttagta gctggggtta catattcata tataggaatt
    23941 agtaattata acgaagtttt atacagtgaa gctggtctta accctaaatt atatataaat
    24001 aatgaaatag ttataaaagc tatattggtt ggaataattt ctgtttttat atcttgtatt
    24061 ataccaactt ttaaaatcgg caagatatca cctattgaat caataataaa tacagacaaa
    24121 attaaaaatc acagaagtag acttggaata gacagactag taagcaaaat atttggattt
    24181 tatggattta tgggacttaa aaatattgga agaaataagg ttagagctgc tatatctatg
    24241 ttgtctattg ctttaggagg atatatattc attactacat tctcaagtat gcaagaagag
    24301 gtcaacagta aaattgaaga tatgcaaaat agatataata taacaatgca atttggagta
    24361 aatgcagacc cgcaaatatc aaaatacacg gatagtgatg tagagaatat agaaaaaatt
    24421 aagggtgtta aaagtataca taaaattcaa ataacaaatg gaatgtttac ttttaataat
    24481 aaggatataa acaaagaatt tacaacctat aatggagtag aaaaaaaaga gataatggaa
    24541 gataaattag ttttaaagct ttatgatgat tatatagatg aaaagttaaa tgactttgtt
    24601 gaagaaggta gcttagatga tattaaaaag gacactgatg gatatccaaa tgtagcagta
    24661 tataattatt tttatgatat agttgatgat catactataa agcctatatt caaagaatta
    24721 aatataggag acataataac tgtaaaaatt cctgttgaag aaaataatac aattgtttat
    24781 aaagagatga aggtaagagt ggctgtaatt ttaaaggatg aatggattgc tatgggagat
    24841 ggcgatttta tgccagatct agaaattata acaagtaata ttcactctaa aaacataaca
    24901 ggagaacaaa aatacactca attaggcata aatttagaaa atccatatga tgagattgtt
    24961 aataatgaga tacagaaaat ttcaagaagt ataccagggt ctctatttaa tagtagatta
    25021 agttatcatg aaatgtcaga aagtatatat aaaagttaca ttaaatcaca aatagcaata
    25081 attgcactag ttttaataat agcatcaatt aatattttct gtactataag aacaaatcta
    25141 ctaattagaa aaaaagaaat ttcaacgctt agagcattag gtgttagtct taaaaatatg
    25201 aaaaaaatgc ttatatatga agcattagca tatgcagttt ttagctttat aatagctatg
    25261 attccatcat ttataaatct agttaaattt acaaattgga ataatgatgc gtatataaac
    25321 tatggaatag agaattttat gtcttttaca ttcccaatta aggaatcttt actattcttt
    25381 gctatatctg tatttgtatg tttaatagca gtagttgtaa gtaatagaga ttttgaaagt
    25441 atgaatatta ttgaaggtat aaaagagaat gaataatttg ggaggtaaat gtatgaagat
    25501 gttaaaagtt atagatatta aaaaaatata tggaaaagga gaaaatgaaa taaaggctgt
    25561 tgatggaatt actttagaga tagaacccca taaatttagt gcaataattg gacaaagtgg
    25621 ttctggaaaa agcacattac ttcactgtat ggcagggctt gataaaccaa ctttaggtaa
    25681 tgtatttatg gataatttag atttatatac tttaagtgat gaaaagctat ctaatcttag
    25741 aagtaaagaa tttgggttta tattccaaaa ctataatcta atacctgtta tcaatgttta
    25801 tgataatata gttcttccta ttttaattgc taaaggaaag atagataata attatattaa
    25861 tgatttaata actaagttag gattagaaag tcagattaat aaatttccta atgaattatc
    25921 aggtggtcaa caacaaagag ttgcaatagc aagatcactt gccaataagc catcaataat
    25981 ttttgcagat gaacctactg gaaatttaga cagtaaaaca actaatgagg tgatgaattt
    26041 actaaagtat tgtgtaaatg aatacaagca gacgttggtt atgataactc ataatgatga
    26101 aatagctaag atggctgatt gtataataac tattagtgat ggtaaagtca tataaaatgt
    26161 attgtataaa ggtttaatcg tgggtattat tcttgtgatt cttctccact atttaaatcc
    26221 acgaaataag gactgtcgaa tgagtaattt taaattgctg tgcgacagtt ctttttttac
    26281 ttgcttttca ttaatttata tttatgcaaa atttgagctt gaaaacggtc ataagaaata
    26341 aaggtataaa aataccattt taaaatagaa taatttacaa agtgtaagat taaaaaatag
    26401 ttttgagagg atgaatatac atactacatt tattaatgat tatcttattt gtgaaactta
    26461 gaaatactat aggctaacac tacaggtata aactctgaaa gaatatcttg attgcctctg
    26521 attagaattc ttttaaagtt catactatgt tttatttggg tgaaagctcc ttatgcctga
    26581 atacttctat tcattctaag ttcacatcct tgtgtaccag tgattctttc taaattatct
    26641 taatgccaaa atcttgttcg catttcaaaa taaagtcagg tagctacttc aacattttag
    26701 ttatatttct tcaatagttt gtctccaaac aaaggtacat ctattagcaa cagatttaat
    26761 tttatttata tcaataaata atatatactt tgaaacttca ttaatatcac caagataatt
    26821 tataagttaa actattatat attatcttca ctaactacat cctccatagt tttacacttt
    26881 cttaattcag cttggtaatc taaatctgta ataattattt ctctttgatt tttcatttct
    26941 ttgcctacaa aagtttaata tgaatattat tctatactta ccaattttac gttcaaaaaa
    27001 gactgtagat aagtctgcat ttacacaaac ctacttatag gtctcttttt tgaaattaat
    27061 ttagcatgaa ctatagtaag agcatcttta aattcatatt tacaagtata caaagttata
    27121 attttatctg aaatatctac ctttatatca gatttgtgta ctgattttta ggttatataa
    27181 ttcaaagaat atttaaactc
//
LOCUS       DAKBNN010000029        26476 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000029 DAKBNN010000000
VERSION     DAKBNN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.029"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            342..1163
                     /gene="yidA"
                     /locus_tag="OUB02_001114"
     CDS             342..1163
                     /gene="yidA"
                     /locus_tag="OUB02_001114"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCT8884886.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCMNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1300..1449)
                     /locus_tag="OUB02_001115"
     CDS             complement(1300..1449)
                     /locus_tag="OUB02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884887.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1456..1941)
                     /locus_tag="OUB02_001116"
     CDS             complement(1456..1941)
                     /locus_tag="OUB02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884888.1"
                     /translation="MSQIEKYNSLNNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1954..2706)
                     /locus_tag="OUB02_001117"
     CDS             complement(1954..2706)
                     /locus_tag="OUB02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884889.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2706..2843)
                     /locus_tag="OUB02_001118"
     CDS             complement(2706..2843)
                     /locus_tag="OUB02_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8884890.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            complement(2883..3512)
                     /locus_tag="OUB02_001119"
     CDS             complement(2883..3512)
                     /locus_tag="OUB02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8884891.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3473..4036)
                     /locus_tag="OUB02_001120"
     CDS             complement(3473..4036)
                     /locus_tag="OUB02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884892.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4046..4270)
                     /locus_tag="OUB02_001121"
     CDS             complement(4046..4270)
                     /locus_tag="OUB02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884893.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4275..5366)
                     /locus_tag="OUB02_001122"
     CDS             complement(4275..5366)
                     /locus_tag="OUB02_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8884894.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5395..5853)
                     /locus_tag="OUB02_001123"
     CDS             complement(5395..5853)
                     /locus_tag="OUB02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884895.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5846..6931)
                     /locus_tag="OUB02_001124"
     CDS             complement(5846..6931)
                     /locus_tag="OUB02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCT8884896.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6958..7143)
                     /locus_tag="OUB02_001125"
     CDS             complement(6958..7143)
                     /locus_tag="OUB02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884897.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7305..7637)
                     /locus_tag="OUB02_001126"
     CDS             complement(7305..7637)
                     /locus_tag="OUB02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCT8884898.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7893..8729)
                     /gene="nadC"
                     /locus_tag="OUB02_001127"
     CDS             complement(7893..8729)
                     /gene="nadC"
                     /locus_tag="OUB02_001127"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCT8884899.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8710..10011)
                     /locus_tag="OUB02_001128"
     CDS             complement(8710..10011)
                     /locus_tag="OUB02_001128"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCT8884900.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(10024..10938)
                     /gene="nadA"
                     /locus_tag="OUB02_001129"
     CDS             complement(10024..10938)
                     /gene="nadA"
                     /locus_tag="OUB02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCT8884901.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11356..12813
                     /locus_tag="OUB02_001130"
     CDS             11356..12813
                     /locus_tag="OUB02_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HCT8884902.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13036..13464
                     /locus_tag="OUB02_001131"
     CDS             13036..13464
                     /locus_tag="OUB02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884903.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13524..13832
                     /locus_tag="OUB02_001132"
     CDS             13524..13832
                     /locus_tag="OUB02_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HCT8884904.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13979..15001)
                     /locus_tag="OUB02_001133"
     CDS             complement(13979..15001)
                     /locus_tag="OUB02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT8884905.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15176..15682)
                     /locus_tag="OUB02_001134"
     CDS             complement(15176..15682)
                     /locus_tag="OUB02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCT8884906.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15742..16443)
                     /locus_tag="OUB02_001135"
     CDS             complement(15742..16443)
                     /locus_tag="OUB02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884907.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16661..17731)
                     /gene="buk"
                     /locus_tag="OUB02_001136"
     CDS             complement(16661..17731)
                     /gene="buk"
                     /locus_tag="OUB02_001136"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HCT8884908.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17784..18359)
                     /locus_tag="OUB02_001137"
     CDS             complement(17784..18359)
                     /locus_tag="OUB02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HCT8884909.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18361..20148)
                     /gene="iorA"
                     /locus_tag="OUB02_001138"
     CDS             complement(18361..20148)
                     /gene="iorA"
                     /locus_tag="OUB02_001138"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HCT8884910.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20209..21468)
                     /locus_tag="OUB02_001139"
     CDS             complement(20209..21468)
                     /locus_tag="OUB02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCT8884911.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKVLDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21730..23730)
                     /locus_tag="OUB02_001140"
     CDS             complement(21730..23730)
                     /locus_tag="OUB02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8884912.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23738..25624)
                     /locus_tag="OUB02_001141"
     CDS             complement(23738..25624)
                     /locus_tag="OUB02_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8884913.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCHSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            complement(25651..>26476)
                     /locus_tag="OUB02_001142"
     CDS             complement(25651..>26476)
                     /locus_tag="OUB02_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8884914.1"
                     /translation="EEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNY
                     NYVKLAYKSIYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEK
                     EINNMRKVKLDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYND
                     TYGHYNGDIALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRI
                     VKKFLNNNLTVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
BASE COUNT         8613 a         4719 c         2788 g        10356 t
ORIGIN      
        1 atattttata acacaaaact aactaatata acaaattacc atcagtctat ttattcaaac
       61 ttctaactat ataatataca tttatttata ttaacaatgc aaacgatacc attatttgta
      121 attaagaatt ttggaattat tgattacaaa gtttttacat tctcatctca cataagtaat
      181 atttttatca aaaaattaat attattaaac tttataaatt acttatctct tgatataata
      241 gtatacaaaa caaaccatat ttcccactga aactttgttc aacactagct gtgtcaacgt
      301 tttcacagga aatttgagta tataatttat tggaggttga tatgtacaaa ttaatcgcac
      361 tagacattga tggaacaata ttaaatacac aaaaaaggat tactcctgaa gtctttgagt
      421 ccattcagga ggcaaaaagg gctggggcaa aagtagttat aacaacaggc agacctcttc
      481 ctggtgtaaa agaacttcta aatcaactaa atttaacaga tgaaggtgat tatgttatat
      541 gctttaatgg agcaattatt caagaagtta aaagtgagaa aatcatacat gatgtagaaa
      601 tgagtttaga tgattttgat tttatatata ataacgtttg taaaaaatat aagactaaaa
      661 tacacataaa tacaatgaca aatttaatta caccaaatga aacgcctggc aaatatacac
      721 ttcatgaagc gaaattaaat aatatagaag tcaaatatat acaaaaagat aaaattgatg
      781 aatctattaa aatctgtaaa attatgatag ttgatgaacc agaaagattg gaagaaatca
      841 tacagcaact acctaaaaat ttattcaata aatatactat agtaaggtct gctccttttt
      901 atctagaatt tttaggtaaa actactaata aaggaactgc tcttaaaaca ttatgtatga
      961 atctaaatat acctatagaa aatgctattg ctgttggcga tgaagaaaat gaccaacata
     1021 tgataaagta tgcaggtctt ggtgtggcta tggggaatgc acgtaatagt ataaaggaaa
     1081 ttgcagatta tgttactgat actaataatg aaaatggagt tgcaaaggtt ataaataaat
     1141 atatacttaa taaggctatc taatcatcta aaaaaaacta aaaatatacc tttagattta
     1201 aatacttttc acatccctac tctatactaa tttatatagt atggatatta cctatcattt
     1261 ttatctaaag gtcattttta gtttttcata tatgtatatc tatgatagta ccttttgttt
     1321 gagtttaaat attttatatg attctactat tgcctcatta atatctaaac aatatatatc
     1381 ttcatcatca gataatcccg cttcgtttgt ccaagcatca aaatcactct tcgtccagaa
     1441 gaaattcatt atatttcagc aactagcagc ccaatttaga tgcttatcaa gatttatatg
     1501 aagcactctt aagtttggat tattcacata ctcaatcttt tcatttttta taattacttc
     1561 tatttcctta cctgtagcac tacaaataga gtttattttt atatcttggt tgaatgtgca
     1621 agaggttcct atagcatcta tagcacacat tgcactgaaa cttctattat ctctcaaagt
     1681 tactttatga tttgttggca atgctgatac aggatatata aaatttatac tattcccatc
     1741 aacaaccatt atatttttat aaataaaata ttgtagtgtc ctttctatat attctttttc
     1801 tatattcaac tttttactta catattcagt cacttcttga agtgtaacac tagaaccttt
     1861 atctattatc atatccataa tagagattct tattttctgt tgtgtattat tcaaactatt
     1921 gtatttttct atttgactca tgtctactcc tctttaaact tcataacact ttgataaagt
     1981 atcagttaat ttgtttttgt actcttcata tttttggctt cctctaattc taggtcttgg
     2041 aatatctata tttatatcat caataattct tcctttatct ctcgacatta ctataactct
     2101 atcactcata tatacagctt catcaacatc atgagttacc attaatgcag ttattttctt
     2161 ctttatccat attcgttcta gctcttcctg taaattttgt ctcatttgaa aatctacagc
     2221 tcctaatggt tcatccatta gtaatacctc tggattacat gctaaagctc ttatcatggc
     2281 cactctttgt ttcattcctc cagataattg atgcggaaac agtttttctt ttcctttcaa
     2341 gtctgacata accaaaagtt cttctagcat ttcttctctt ttttcttttg gtattttctg
     2401 ctgtttcata ggaaatagaa cattacctct tatagttttc caaggaaata aagcataatt
     2461 ttgaaatata aaagctctat ctatgcccgg ttttttaact cttttatcat ttataaaaac
     2521 atctccacgt tctgattttt gaaacccacc tattatagta agtaaagttg tttttccaca
     2581 acctgatggt cctaaaagac agacaaattg tccatcatct atatccatac ttatatcttc
     2641 taaaaccttt gtatgtactc tattatttac aaatgtctta tttatatccc ttacacttaa
     2701 cttcattaat ctgcaaacct ccatctacaa aggattttat ttataaactt taatacccta
     2761 tctattataa atcctattaa agctgcaaat atcataagcc caattaatac atctgtatgc
     2821 attaatgttt gagcttgtac cattgagtaa ccgagaccgg cactcgtagc tataaactcg
     2881 gctcagataa cagacatcca tcctgtactt atagcaatcc ttgaaccagt tagtatatct
     2941 ggaagagatg cgggaacaat tacatttgta aatatcacaa ttggcgatgc tcccatactt
     3001 ctagctgcat tataataatc ctttgatatt gctcttactc cttgaatagt atttaaaatt
     3061 atagggaaaa cccctgaaaa agctattaaa aatattgttg gaccatctcc tataccaaac
     3121 catactatag taagaggaac ccatgccatt attggaactt gccttacaga atctactact
     3181 ggagataaaa ccctttcaaa agttttagaa aatcccataa taaatcctat tggaagccca
     3241 aacaataatg catacataaa acctttaagt actctctgca atgttataaa aagattagtg
     3301 actgtctctg catctgtcac acagaaaaag aaacctttta ttacatctac tggcattgga
     3361 agtaacaaag aactccctat atcttttgct gcaaaatgcc agactaaaaa tataataact
     3421 aatagtatta ttttatataa aatatccata ctaccatcag tttttttctt agttaatcta
     3481 tcgcttttat ctttagaaat tttaatttcc aattgcttat ttcctccata tctatatttc
     3541 ttatattcct gtactctttt aatactttct taaatgtagc tttattcttt aaagcatcaa
     3601 cgcttttatt gtaaagtgtt ataaaatcac tatatgcttt acttttttta aactctttat
     3661 ttacaactaa tacataggta tcataatctc ctaatttagt tgtagatttt cttttaccct
     3721 ctaacccaat tgactttatg caatctatta caacaccatc tactaaatta ctttctaatc
     3781 catatgctag tgcagtccct aataagggag ctacttccac attcttgtag tattttttaa
     3841 ccaattctga ctggtaatct ctattttgaa tgactcctat cttttcagga ttattatcac
     3901 ttaatagaaa tatatcagaa ttttgaacta cagtaccaac aatttcaaat ccactattta
     3961 catttacata ttgttttgca gcatctttac atatgattgc tatatctatt tcctctgaac
     4021 tcagagccca ctgcattgta cttgctcagc aatcattaat tgtaaaaata tccattatat
     4081 cacttacttt tatattttca aagtagtctc tatttaccat ataatttatt acaaaaccag
     4141 aggtatcatc agatacccct attcttaatt ttccttttat ttcttcttta tcttcaaaat
     4201 taaaaaatat tataaatata cttcccaaaa taatgataaa aagtattatt atatttttca
     4261 tattctccat ttatttataa tacctttgct ttccagtctt cataactcat accttcagga
     4321 aactctgggt taactttttc ttttataaat ttgtcaaaat catcagctcc acaagagtcc
     4381 aataagcttg tatcaatcgt atcttcatat ttaggtatat catctaaagc ctttaaatct
     4441 ttatacattt gtaaattatt tttatattct tcacctgtaa ctttccaagt aagagttcta
     4501 ccttctccaa cagtcttctt atagatagtt ctaagtgcaa cttcttcttc tacattataa
     4561 tattttgcaa atattttagc tgcttcagct ggatttgtat atatatattc tattgctttt
     4621 gtatgtgcta gaaccaattt ttttgctaaa tctggatgtt cttttataaa gtttttgttt
     4681 aatgagaatg aacaacagtt atattctttg ccattagctt tttctttata ttgagtagat
     4741 gctattattt ttcctgcacc gtcattttca gctacagaac cccaagggtc acaagatgta
     4801 aaagcttgaa ttttcccagt tttaagagct aaatatttat ctttgtctga atccatattt
     4861 acaacttcat attttgatgg gtcagttggt agacctgttt ctggaccata atcacttgtc
     4921 cataagaaat cactcatatc tgttgcaatt tttttaccta ttaaatcttt aggttctttt
     4981 atatcttttg aaactattaa atattcagaa ccaccagtat ggttgttagc tgctattgtt
     5041 attggcgaac ccttaggtat tgctccaact aaccccttag ttcctatgta acctgcatcc
     5101 atttttcctg ctgccatagc ttcaggaact ttaccatttc caacagtttt tacattaaga
     5161 cctaaatctt tatatattcc tgccgattct gctactggtc ccgcagtcat atggtcacaa
     5221 ttataataac ctactgttat tgtataatca tcttttccag tttttgcttc ttctttttct
     5281 tttggtttac tactacatcc tgttaatata gatgtaccca ttacaccaaa taagattaat
     5341 gcttgaatct tcttagataa tttcatataa atttcccacc tttatgtatt atttttagca
     5401 acatttgtcc atatcttcgc caaataaatc aacaaatttt ttaccctcta ctctgtaaat
     5461 agcatatcct acattaccat ttttagaccc tgatacaatt acttctattg gtgctttatc
     5521 atcaatattt ttaaaggtta tcgtttgatt ctctataggt gctggtattg gctttgttat
     5581 atagtggcta tttttatcag aaactacgac taccatttca tttgaattat tgcttttttt
     5641 atatataact actaaatctt tttttccatc attatttaag tcttcttcag cacaagttat
     5701 tatgtcttct ttataatttt ttctaaaata ttctaatgtt tctttattca catttggaat
     5761 agaagcatct tctttgatgt tatcatcaac tatttttgat aaaatgatta acacaactac
     5821 tatacaggat attataacta tctttttaag catgatttcc tatgacctct ttcatctttg
     5881 gtattattga tttaaatgca aataaaataa ttaaaatagt acatacatac tcaaaactat
     5941 caggtatatt taacattagt ttatttgcca actgtattga agaatttgtt gcatctaaat
     6001 ttactgagtt attattcact aaaagtaaat ttgttatata tccaattatt attgaagaca
     6061 gtgtcaaagt tacagaatat ataataacag ttctttttcc aataagttta aacatactta
     6121 taagttctgg tagatttgta gctgctccag ctattagaaa tgttatcgct ataccaggag
     6181 atgctccact tgcaacaagg gctgctataa acggtatatg tcctacagca catacataca
     6241 taacacatgc aagtattgct atacttccaa gagatagcaa acctggattt cctaaatatt
     6301 tttgaattat actatttgga aaaatagttg ttataaatcc tgcaaaaacc atacccatta
     6361 ctacatattt actaatagta agggctaatt caccaaaaga ccatttaaga ccatcaatca
     6421 tatttcccat aaaacttctt ttttcttctt ctaattctat tacttcttcc tctgaatcat
     6481 tatgaagtga taattcttct tttccaaaac aatttcctaa aaatcctact aaaaatggta
     6541 ctaaaaaacc agctattaga taaatgattg taactttagg acctaaaaga cctagacaaa
     6601 gaactacagc tattggattt attattggag ttgatgtcat aaaagcaagt actggtccta
     6661 aatatgctcc tgaataatac atacttattc caagtggtat tactccacaa ctacatattg
     6721 gtaaaaacat tcctgagata gtacttttaa ttaaagaaga aaatttctta tttccaagtt
     6781 gtttttgaaa ccttcttgga gttaaaacat tgtgaagtac acctgccaat ataaaactta
     6841 tcataagcca tcctgatgct ccattcagtg taactataga agctactata gtatctttaa
     6901 ctccatttaa aacaatttct aatatattca tttttgatac tcctaatttt taaactttta
     6961 attattgttt atagcttcta ctattgccct ttcaattgta tctttattta atctatcata
     7021 tttctttttc tgatttatta ttaaagttcc ttttgttata atcccatatt tttttatata
     7081 cgaaaaatct ttccctgctt gatataattt tatttccaat ttatcagcat atttttctga
     7141 catttttttt ataaacactc catgttcatc acaagatggt caagtgttta taaattcaac
     7201 taaaatttta gacatttgtt aatcccctac tttaaacaat ttatctttta cttaaaataa
     7261 tccacagaaa ataaattttt ctatggattg tatcctatac taatttataa cgttataact
     7321 tggtccacac ctgcactgcc taaacaagca tataggtttg ggaaacatcc aattgtagct
     7381 ccatcaacca aattattctc tatacctctt tctatagcac attggtcaca agccattatt
     7441 atcatatcag ttttttcatg taatttactt agtctctcgc ctacaggatt acctttagat
     7501 aaaaataagg tattatccat aaagaaaaac atccctagaa cgtctactcc atgagttcct
     7561 tgttcaagtt gtggtattat cattttgtct agaattttat gtgtattagt actactaaaa
     7621 atataagcta ctttcatttt tattccccct atacttttta tattttacaa aattttacag
     7681 atacaattat attataagtt tattcttatt tatctgtaaa atagatatta actatattat
     7741 taaaagtgta tatagtattt atctattttt attaaattat tgattgattt ttttctattt
     7801 ttattaattt taatgaataa aaaatagact ccttaaattt ataaaacaaa tttaaggagt
     7861 ctattttaat taagtttatt tcaatataaa atttatatat ttctcaaatt cttcatactt
     7921 atatcgagag ctttaacaga atgtgtaagc gctcctacag aaacaacatc cacaccaatt
     7981 tttcctatat cttctatagt atctaaattt acatttccgg aaaattctac aatggctctt
     8041 ccatttataa tatctactgc ttgttttgcc atttccaaac tcatattatc caacattatt
     8101 atatctgcat tagcttctat tgcttctttt accatgtcta aagtttccac ttccacttct
     8161 atctttctaa caaaagaagt attttttcta gctaattcaa ctgccttctt aacactacca
     8221 gcagccccaa tatggttgtc tttaagcaat attccatcag ataaattaaa tctatgattg
     8281 catcctccac ctactttaac agaatactta tctaatattc taagattagg tatcgttttt
     8341 ctactatcta ataattttgt atttgtacct tcaagctttt caacatacct acttgtaata
     8401 gtagctatac cactcattct ctgtaaataa ttcaatgcta cacgctctcc tacaagtaag
     8461 tttctagtac tacctgttaa tacagctatc ttttcacgat tctttactct atcaccatca
     8521 tttttatata attttacatc tacatcacca agtatctcaa aaactctctt aaatacacct
     8581 aagccagcta ttataccatc ttgtttacaa attaaatcta cagtagattt agaattttca
     8641 tccacaatag aattagttgt tatatcttca cttggaacat cttctatcaa agcatcctgt
     8701 atcattctat caataatcaa ataattcatc gccaaaatcc tcacttgttc tttcaatttc
     8761 ttttatgact aatattttat tttctttttt tacctgttct atatctatat ccatatctgg
     8821 tacatctata aacttaactt ttacattatc tataactgag tttatatttt tagctgctct
     8881 ttttgaaaat acaagccctt caagtaatga attacttgcc aatctattgg ctccatgaac
     8941 acctgtacaa ctaatttcac caactgcata taaattttcc atagatgttt ttgaatccaa
     9001 atctacatgt atcccgccca taaaataatg ttgtgcagga gataccttta tacattcttt
     9061 cgttatgtct gttcctctag ctaaacattc attataaata aaagaaaatc tattaattaa
     9121 atattcacta tctaaatgtg ttgcatctaa atacacataa ggtaatttat ctttttccat
     9181 ttgctcaaaa atagcttttg aaactacatc tctaggaaga agttcatcca caaatctttc
     9241 tttattttta tttaaaagtt tagctccctc tcctcttaat gattcagata taagcattct
     9301 cctaccttca ttattttctt cataaaaggc tgtaggatgt atttgaatat attctaaatt
     9361 ttctattttt atattatttc ttaaagctat tgcaattcca tcacctgtaa gatgtctttg
     9421 attagtagaa tttttaaaaa gcccaccaat tccacctgta gctaaaacta ctatttttga
     9481 aaaaacatga atctcttttt tagatttaaa aattctagct cctatacatt tattgccatc
     9541 ttttaatata tctaaaagat aggcatcttc tataagagtt atattttctc tagttttagc
     9601 ttcttttaat aaagttttaa aaacaacttc tccagtatta tccttactat gaactattcg
     9661 atttacacta tgtgctcctt ctcttgtgta atctatctct ccattttctt tgtctaaagg
     9721 cataccatat tctactatct gccctacatt ttctatagat tcatctgcaa gtacttgaac
     9781 tgcttcaact ctatttttat actgcccagc cttcattgta tcttctacaa aggattctat
     9841 atcatcaata tttctagcag ttgaaattcc accttgtgca agatatgtat tgttgttttc
     9901 aattgttgat ttcgatacta ctagcacatt taaactttta tctaaattta atgcacagta
     9961 taatcctgaa actccacttc caactattaa aacatcttgt tctaaattca taagtctatc
    10021 cctctatcct tctgctaatt tgtgcatgtt aagcagtgaa tttaaagcct tttccatgat
    10081 ttcatcttct acaataactt cattttccat atttttaagt gtactatata aattttctaa
    10141 tgttgtcttt ttcatatctg tacatagtat ttttccacca ggaatataaa agtttttatt
    10201 aggattattt ttctttaatt catgtaatat accttcttca gttgctatta taaagtcttt
    10261 gtttttacta ttagtagcat attttattat tcctgatgta ctacctacat aatctcctaa
    10321 atctcttatt tcttttttac attctggatg tactaatact tctgcatttt tatacttatc
    10381 ttttaactca attatttctt cagctcttat attgttatgg tatttacaat atccgtccca
    10441 aaatataaag tttttatctg gaaattgttc tgcaatatat cctcctaaat ttttatctgg
    10501 aaggaatata atttctttat tatctatgtt ttctagtatt ttaattgcac tagaagatgt
    10561 aacagaaaca tcacaatgtg ccttaacttt agctgtagag tttatataac atacaaccaa
    10621 agcatttgga tactgttgtt ttaattttat cacgccttct tcatctacca tatctgccat
    10681 agcacaccca gcgttactta ctggcatcaa tacagttttt tcaggtgata atatcttagc
    10741 actttctccc ataaacctta ctccacaaaa tacaaccact tcttctttac aatctctagc
    10801 aatctcactt aaataatatg agtcaccaac tgcatctgct aattcttgta tttctggtgg
    10861 ttgataaaaa tgggcaagta ttattgcatt tttttctttc tttagctctt tgatttgata
    10921 tgttaaatct ttatccataa ttgcaccgct ttctatatgt actagtgtat atacacctgt
    10981 aaactcatta tatcacttga ctattctttc ttcaagaatt ttaaacctct taatattgtt
    11041 aagatataga catttatatt ctcaattgat tttccttttt tctccatcta tttaaactaa
    11101 tttttatatt ttttaatttt tttgtaaaat attatcagaa ttttgtttat attctttatt
    11161 gctataacat tgaaaatatt accaaatacc tgtatactaa gtacatggtt atgtatatgt
    11221 atttatttct gaaattatat tagttgtaat actttataga atttgtaggt tgtccagaat
    11281 taaattcaat ataaatctac acatttagat aatatgtata caaagaccaa aaaattttat
    11341 aaatggggtg atattatgac aacattctta atcggcttgg ctatattact aataggaggt
    11401 gctttatacg gtgcttattg tgaaaaagta tttggcccag atgacagaaa aacacctgca
    11461 cttgctcaaa gtgatggagt agactatgtc ccaatgaaaa aatggaaaaa tagtctaatt
    11521 gagttgctta atatagctgg tacaggacca attctaggac ctatccaagg tatattgttt
    11581 ggtccaatag cattcatact aatacctatt ggctgtgtat ttggaggagc tttgcatgac
    11641 tatatgagtg gaatgattag tataagagaa aaaggagcac aaatgccttc tcttatcagt
    11701 agatttttag gtaataaagt atttcaagtc tataatatat tcctttgtct tttaatgctt
    11761 ttagttggtg cagtttttat ttatacacct ggggatttag ttgttactca aattttaaat
    11821 atgaaatcga ctataaataa tccagttgtc tggatagttt atggacttat ctttctatat
    11881 tacttgtgtg caacactatt tccaattgat aaaatcatag gaaaggtata tccaatattt
    11941 ggagctattc tacttttatc tgctgctggt gttggaattg gaatctttac acaaggttat
    12001 gatttagcta atttaagtct tgcaaattgg aaaggtattc atcctgatgg tattcctctt
    12061 attcctacat tctttgttac agttgcttgt ggtattgttt ctggattcca ctcaactcaa
    12121 gctactctta tagcaagatc agtttcaaat gaaaaagaag gaaaaactac tttttataat
    12181 atgatgattt tagaaggttt aattgcaatg atttgggctg cagctgctat gggtatttac
    12241 aataaaggaa tacctaagga attagtaggt tctcctgatg taataggttt ggttgcaaga
    12301 gatttacttg gttctattgg tggtatcata gctataatag gtgtaattgt tttaccaata
    12361 acttctggag atacagcttt aagatcttta agattaatgc ttgcagatta ctttcattat
    12421 gaccaaaagg aaaaaaaaca tcgtgttata ttgtcaatct gtatatttat tcctgtcata
    12481 gccatcctta tatttgcaaa gcttagtgct tctggattta atatattgtg gagatacttc
    12541 tcttggagta atcaaactat tgccatattt gcatttgcaa tgattacagt ttaccttata
    12601 ataaaagaaa agaattatat tataagctta attcctggta tgttttattc ctttgttata
    12661 ttcagttata tatttaatgc tcaaattgga tttaacttaa acataaatat atcatatgtt
    12721 ttagctgcca tatttactgt attatatgct attttaactg tacgctctgg tagaaagtta
    12781 aaaagcaaag cagatactaa gttggctgac taaatctaaa taaaataata cttagaataa
    12841 attttattaa aaaatgataa aaaagtaggc aatctcaagt tagatttgcc tcttttttat
    12901 ttaatacagt aataaatact taagtattta ctccttattg gaatatttat cctaaatctc
    12961 taatataaat aaataaaagt tttaaaataa tctattcgtt tatatactta aaataaattt
    13021 tttggaggaa tacttatgaa taataaatta catgaacttg aaaaaaatct accagaaaca
    13081 tcttgttctt tttgcacaca tctttcattt aaggggccta gcctagacta tagatatgat
    13141 attagatgtg ttatttcaga tacaaagccc gattttagag gatgttgtga atattttgaa
    13201 ccagaatata cagaattaaa tacaggagat ttagacaatc tatacataaa ctttcttgag
    13261 acatgcctca aggtaaatta taatgattac atcaattcta tgtattggaa gttatttaaa
    13321 gaaagaaccc tactagaaaa caattttaaa tgttctatat gtggatcaac tgaaaatgtt
    13381 gatgtgtatc atgttaataa aaatttagga agggaaactt ccaaagatgt agttgttaaa
    13441 tgtgataaat gtaattgtaa ataagtgtat aaaatttaga taacttcaca taataagttt
    13501 aaataaaact taggaggtta tttatgaaaa ataataattt aaattgtgct gctactaatt
    13561 gtgcttataa tactagtgga tattgttatg ctggtagtat aaaagtagat ggaatgcaag
    13621 caactactac agaaaatact tattgtgctt cttttgaaga caaatataca tctggtataa
    13681 ctagtcgttc taatgaaact aaccaagttg atacagataa cattcattgt gaagctgtaa
    13741 aatgtaaata caataaaaat gaactttgca aagctgaaaa agttcatata aatgaaagaa
    13801 atgcaagttg tgaaactttt gaaatgaaat aaatttcttt ttatttagtt ttatagtatt
    13861 tatacaaagc aaaattgaac ctatcaaata gatgtcttta ttaatgatgt ctatttatag
    13921 gttctttttt aaaacaattc ataatttata tataaaaata aattattatt ttaatatatt
    13981 acctagaagt aaatttaaca cccagaatat ccataaggaa cataaatatt ggaactccaa
    14041 ttagtaatcc ccatacacct aaataatgct ctcctacaag taaaataata aaaacaaaac
    14101 aaactggaag tttagttcta ttcgacatca acttagggtt taatatgtat gtttctactg
    14161 catgcactat acaaatcata agtaaaactc caaaaacctt tgttattcct cctgtattaa
    14221 atgctattac agttaatgga atcaaagaca ctataactcc tgctactgga ataagaccca
    14281 acacaaaaat cataaatcct aaagcccata tttgagggaa tcccatgaaa cctaagaaaa
    14341 tcatagaaac aacagaattt ataaaggcaa tcataacttg tactttcatt acttcaccaa
    14401 aatttttaac aaagctcttt ccaaaaaata caagcgaatt ataaataaaa gaaatcttac
    14461 tatcagaaag tttatctcca aaatttttaa tttcattttt ttcaagtaaa aggagtaaac
    14521 tcagtaaaaa tgccaataat atatttactc caaaacttcc aacttttgtt gctgttgatg
    14581 ctataagtag ccctgcttgt gaaatatatt tattaaaatc cagctttgag acaataggat
    14641 acaattttgg tcctaaagat tgagcaaatt gatttacatc aaaatcacta aaaaccctac
    14701 tcaactctgt aagttgaact ataattattg gtacaactgc atagctaatc aatactaaca
    14761 atatagcaaa tagtgcatat aacactataa ttatcaatga atctggaata tttaaagaag
    14821 aaaacttctt aattcgtttt cttgtggcta caagcaagtt atagaaaata aaagtcataa
    14881 taaatgttaa aagcaccata tttaacatat accaaaatgt aattagaata attactacga
    14941 cagatataga aacaatttta tattttgaac tgtcacgtgc caatcctttg ttatcttcca
    15001 tagtttaaat cctccccaaa tttttatgac tttataatga ctgccctttt tattttaatc
    15061 tcaaaaaaca agtagaaaaa tactaaaaat aacttattaa ttttcataaa acaatattaa
    15121 aatttagaga agatacattt taaataatct tacttatact gaaatctgct ctacatcata
    15181 tatttaattt attattatct atctcatata gcaattcgta tatctctgga tattcaaaaa
    15241 acaaaccctc ttttagtaat tgtcttattt catctgtgct tacccacttt atatcactta
    15301 cttcttcctc ctgtaaaata gctttggata tatcatattc ttttttaact aagtatacat
    15361 cccaaagagt atcttcatga atcatacttc taaaaacttt catctcatct ttagttatat
    15421 ctatccctat ttcttccttt gcttcgcgaa ttgctccttc taaactgtct tccccagaaa
    15481 caatacatcc agttgtcatt ccccacatat ttggcaatgt ctttttactt ttagaccttt
    15541 tctgaattaa aatttgagag ttactattta aaatccacac ttcaactgcc aaatggtaat
    15601 aaccttcttc aatagaatta cctcttttta taacatttcc tgttttaatt ccatcagcat
    15661 tatataaatc ccataattcc atttcaaaat ctccttgact ttactataaa ttagataaac
    15721 tacaaatata ttttagcata attaattctg ctcatttttt attacaatac ttgatttaca
    15781 tttatgttca ttgcttttct tttccttcca ttctcttata tcatctataa taaatctatg
    15841 tttcaacaat tttgtgtcaa atttaaatat tctaaacact atttgtaata catatcctaa
    15901 acctaaagca cttacaagcg taccaatacc tacagaacct cctaataacc acccaacaac
    15961 taatgctagt atttcaagaa ttgttctaac aagacgtaca gatttattag tttttttctg
    16021 aagagcaatc ataagcccat ctctcggtcc acttcctaaa caaacaccta agtatagaaa
    16081 acttgctata gctgccaaaa taattcctat aacaaccata aacacaccta tataagtgtt
    16141 gcttgcatga ggtatcactc cagaaaatat tactaaatca ataaaaattc ctataaatgt
    16201 catgttgagt aaagtacccc atcctatatt ttcaccaaaa actgcatcca atataacaat
    16261 tacaactcct accattatag aagcctgacc tatagttatg cctagcttta tagataaacc
    16321 ttggtgaagt acatcccaag gcataagacc tacatgtgca ttaatcataa gcacagaaga
    16381 aattgcaaat ataaaaaaac ctgaaaataa tcttatcata ctttttaaac ttatttttcc
    16441 catcaaaaat cactcctcat aataataata acaataaata ccaataaaat atatatataa
    16501 atacgaaatt ttacagaatt atttattagg ttaagataaa attttaatta taaaaagtgg
    16561 caatccaaat tttatacatt ttcacctata ctctaagctt tcgtgtataa aaaacggata
    16621 taccactttt ttagtatata aacattttta gtttttctta ttaattttca ctataaattc
    16681 tagcttcttc ttctccattt agtactctta aagttccaaa agcaagtgat tccaactcat
    16741 tttctcctgc cataatttct actggtgcta taaactcaac tttcttttta accattgaag
    16801 taaacatttc agaatatgca attccacctg ttataattat agcatctacc tttccatcta
    16861 ctacagttgc taactctcca ataccttttg ctaactgata agccattgct tcataaataa
    16921 tttttgcttc ttcatttcct tctgctatca ttttttcaac ttctctagca tctacagtat
    16981 ttaggtatga aactatacca cctttacctc ttatcttttt agtcatttct ctttctgaat
    17041 attgacctga ataacaggca gccactaatt ttgtagcagg tactcttcct gacctttctg
    17101 gagaaaatgg tccttcatca tcagatagca tatcaaccat tcttcctttt tcatgaagat
    17161 aaatacttac tcctccacca atgtgagcta ctattaagtt taagctctta taatcttttc
    17221 ctttatcctt agcatatttt aaagccattg ctctagtatt taatgcatgc cctgcacttg
    17281 atctatccat tccacaaagt ccagatattc tcgctacatc tataagctca tctactgcaa
    17341 cagaatcata aatatatgag ttgattccaa gaggttctga tattgcatgt gccacaacag
    17401 cacctaaatt ggaagcgtgt tcaagtactg gtctatgtct tagtacatca atcatttctt
    17461 catttactaa atatgctcct gattttactg gtggtaaaac tccacctctt cctactattg
    17521 cactaagaga ttttaagtct atactatttt ctttaagaat attcaaaact gcttccttac
    17581 gcatttcaaa ctggtcttgt atagtattat attttgcaat ctcttcagct ggatggtcta
    17641 tcgtcttcac aagaatttgt tcttctccat catatactgc tatttttgta gaagtagaac
    17701 ctggatttat ggctaatatt ctgtaagtca taattttctt cctttcacct taatttctat
    17761 agtctattta tatctattat aaactatttg ctaaccattt ccataccttc atggaaagct
    17821 tttatgttta attcaacaaa ttttggttta acattttcac ttataatttt ttcccaatca
    17881 atagactcta attgcataga tttaactaga catcctaata aaataacatt cattattttt
    17941 gaatttccta gttcttcagc tttatcagct gcatttataa cttttgcatt taatctactt
    18001 aactctgcat ctatatcact ttcttgatat tcagctcctc cgattaaaac agtcatagaa
    18061 tctatcctat gattatttac taaagctttt ccttctggtt ttaaataatt gaaccatctt
    18121 aaagcttcca ttttttcaaa agatactaat atatctgctc caccaatttc tataactgga
    18181 gaatgaacac aatctccata tctaacttgt gaggaaactg aacctcctct ttgactcatt
    18241 ccatgtattt cactcatctt tacatcatat ccagcttcca tcaatcccat agttaataat
    18301 ttactagcaa gtatagttcc ttgacctcct acacctacca aaagtatact cttagtcata
    18361 ttatttttcc tccttaacta ttgcttgttt tggacaaact tgtgcacata ctccacatcc
    18421 tacacactga tttctatcta tatttgataa cttattctct ttatcaaatg atagagctgg
    18481 acatccagtt ttcaaacata atttacatcc tatacattta tcatggtcaa ctttacactt
    18541 agtcttaaat ggattattaa attcttctat atcttcttta gagaattttt taagtacaca
    18601 tggccatctt gttattatta ctgatggttc atcttctata gctaaagccc aatctagtgc
    18661 ttcatttact tcttttaaat tattagggtt tataactctt acatgttcta ttccacaagc
    18721 tttaactaat ccttctatat caacttcttt agttttagca ccttgtaaag tatatcctga
    18781 acctgggttt tcttggtgac ctgtcattcc agttattcta ttatctaata taaccgaaat
    18841 agaattacct ctattgtaaa caacatctaa aagtccattt attcctgtat ggaagaatgt
    18901 tgagtctcca agtacaccta ccaatctttt ttcattatca tccttcatat taagaacttt
    18961 ttgagctcca tgtgcagttc caaaagcaga acccatacaa acaacatagt ctattccatt
    19021 atatggagga gcaaatccaa gagtataaca acctatatct ccaccaacta ttaaattttt
    19081 tctctttcca agttcataga agaatcctct atgtggacat cctgcacaga aacttggtgg
    19141 tcttggtgtt actaattcag atttgtattc tatagtatca tttgtttttc caaatatagc
    19201 ttttcttaaa acatctggtg tcatttctcc atatggtggt attacatctt ttccaataca
    19261 atctattcca tatgctttta tttgttcttc tataaatggg tcattttctt ctataacata
    19321 tattttatca actttttctg caaattcttt tattttttca taaggtaatg gatttgtaaa
    19381 tcctagtttc atatatgatg cattttttcc aaaaacttct ttggcaaaat tacaacacat
    19441 accagatgct ataacaccta tttttgtatc atttatttca tagtagttta aatcagtttc
    19501 atttgagaat tttttaagaa tttccattct ttcttcaaca acgccccttc ttatttgagc
    19561 atttgctgga acagttacca ttttcttagc atttttaaca tactctttaa tttcaacttc
    19621 ttctctatca tagcactcca ctaatccttt tgaatgacaa agtcttgttg taactctata
    19681 taaaactggt gtatcgtatt tttcactaac ttcaaaagct tcttttatca tatctttagc
    19741 ttcttgacta gttgatggtt caaataaagg tatctttgca aattttgcat acatcctatt
    19801 gtcttgctca ttttgagatg aatgcattcc cggctcatct gctgtaataa gtaccatacc
    19861 accatttacc cctgtatatg cataagtaaa tattggatca gctgcaacat tcaaaccaac
    19921 atgtttcata gaagccattg ttcttgctcc tgcaattgaa ccccctatag cagcctcaag
    19981 tgctaccttt tcgtttggag cccactctgc aactattgca tctttatatg ttgctatatt
    20041 ttctaatatt tctgtacttg gagtccctgg atatgcagat gcatatttaa caccagcctc
    20101 ataggctcca cgtgcaattg cttcattacc tgtcatcaat tgtttcatat aaaattccct
    20161 ccatctacaa ttattaaatt catagtccac ccaatttgtt caatgaaatt aattatttat
    20221 ttttacttct aaactttcaa gtgcttcttt tataatactt ggtgctttct tacatttatc
    20281 ttcagaaact gcacaaactg caaatcttaa gcccattttt aatggaactg caaataaatt
    20341 atgtttagtc aattcttcac atacttcttt tggattatca catggtatac ttacaaagaa
    20401 acctgctata tatggaagaa tttctaatcc tactctttca gcttctttta caaatacatc
    20461 tgctcttcta gtaagcatgt ttttgtatat ttctttttca tcctcatatt ctttgaattt
    20521 tttagggtca tttacaatgt tagataatac tgccatagca cttctattac aatttgacca
    20581 atttgctcta catgaatgaa cacatgagta atggaactct tcagctacat cttcacttga
    20641 agatatacaa attatagctc ccattctcat accataagct gtaaatcctt ttgacatact
    20701 gaatccaacc aatacgagaa cgttttcagg caaattagaa aatttcttaa aaaactttct
    20761 acatacatca tcatcacctg caaagtcaat atatgctgaa tctacgaaaa atattatctt
    20821 tttgtcttta tcttttgcga cttcttttga taagtctaat attttatccc actcatcatc
    20881 tgcaacacta tatccagttg gattatgcgc tggtgtgttt ataattgtaa agactctacc
    20941 ttgtttttct gctatattta caaatttttc tttaaacgac tcaaaattaa accttctatt
    21001 ttcatcaaaa agttggtaat taactacttt tctattagcc tcttcagcta tacttacata
    21061 tggactccag aaccaatcag atgttaatac ctcatctcct tcattagtat agttccatac
    21121 agctaatttt attgcaccac ttcctcctgg tgaagctaat actcttatat gtccttctgg
    21181 taagtaatct ctgaaaaata cttttttaac tgcttctaag taatctggtt gaccttcaag
    21241 agctgcatat gcacctatct cacagttatc taatacttta tattcttcat atacagtttt
    21301 cattgttatc aattttcctg aatcatccat tagagcgcct attgttgcat ttattacatt
    21361 atccatacca aaagccttct ctgctgcttg agctctgcca gatatgctaa atataacatc
    21421 attttctttt ggccacattg catgcttacc aaccatactt tgactcatac catcacccct
    21481 aaaattgtat ttactcttac attatatatt aagcaattac tatgccaata aatcccactt
    21541 tatgtaaaat tgattttgtt tagaattatt atttttttca ttattaaatt aagtgtttta
    21601 actatttttc ttataaatat atatgcctgt ctaaaaaaat aaaaatttgc aaaaaagtaa
    21661 tgttaatttt tcttaacact tgttttaaaa aattaacatt acttcaaaat aatttatatg
    21721 taattatatt taaatatttt tattgtattt cttccataaa gatgtcctgc ttattccaag
    21781 aacttgtgca acttcatttt tagaaagtcc ctgagcaaaa agcatactta ttattttttc
    21841 ttcaacatac ttatttactt cttttatatc tatacttatc ttaccatcat ttatcttact
    21901 tatatttata gattcatctt ggacttctac acttgctgtc gtttcagaat taaatttgtc
    21961 tacttcagag ttaggaagta attttttagt tttaaaatct ctatcttgtc tcaatttaat
    22021 tttacctgtc acaacatatt tttgagctat tgtctttaac tctcttacat ttccaggcca
    22081 gctataatta tatagcttat ccatttcatc tttagtcaaa acaaaatttt catctatact
    22141 gtttattcca ttgagaccat catcctttaa tacttcattt acaaaattgt taaacaaagg
    22201 tattatatct tctcttctgt ctcttaaagg aggtatattt atctctaaac tactcaatct
    22261 ataaaataag tctgctctaa atgtacccat aactattttt tcggttaaag attcattcgt
    22321 tgctgctata atccttatat ccagaggtat tatataatca gaaccaattc tcattatttg
    22381 acgttcctct attactctta atagttttgt ctgaatatta aaggatagac tatttatttc
    22441 atctaaaaaa agagtccctc catgagcaag ttcaaatagc cctctttttc caccctttct
    22501 agcaccagta aatgcacctt cttcatagcc aaaaagttca ctttcaagta aattttctgc
    22561 tatagtagca caatttatgg caacaaaagg cctatctttt cttttactta tattgtgtat
    22621 actatgagct ataatttctt taccagaccc actttctcca taaagtaaag ttgtataatc
    22681 actttttcca atttttttag cttcttcaat aaattcttta gttaatttat ctttaaataa
    22741 aaaatcatca aaagtatatc tagcatagag acctttttga ttcaagtcaa atcttatttt
    22801 tctttctaag ttttgtaact tggttatatc ttgtattatt cctaaaactc cataagtact
    22861 attatcaact tttatcaatg ttgtcctagt attaacaatt agattattta tatttcttat
    22921 ctttctatct tctacgtcct ctttttcatg taaacaatct aacatccact ccattttagg
    22981 aaatacatct aagatgtatt tattaagtgc acattttctc tctactttaa gcatgttttt
    23041 tgcactttca ttgtacaaaa ttatactgcc attactatct atagcaatta ctccatcttt
    23101 tataccatca agtatatttc gtaacacttc attattaaac ttttcttcac ctaatgtatc
    23161 taataatttt ttacaatatt ctacagcttc tcttatagat tcatcactag cagttgcaaa
    23221 tacactatca attccatatt gccttgcaaa agaacatgcc aaacctcctc ctacgattac
    23281 aacttcatct ttttggtcta tatattttac aactttactt cttatctcat acttagattc
    23341 aaaccattct tcagttattt cagtacttat gacatttctc cagccaacat agtcaaaaga
    23401 aacttcattg tctccaagaa tcaatactac ttttttagaa tatttctttg ctatttctat
    23461 tgctcttaaa aggtcagttg acttcatatt cattggtata acaggtacat ttactgtatc
    23521 tattactaaa ctataacctc cacctctacc aattatagct tgggcacctt tttcaacaag
    23581 tatacgacct tgattttcca ttaagtctag gttaagtatg tcgattatta tagttccatt
    23641 ttctacatct tctctatata attcttctat tctttctttt aattcaatat cagaggctat
    23701 aattcctatt ttttttcgca acaaaatcac ctcaatttta aattataatg tattgatttc
    23761 tacctgcttt ttttgattta tataggttct tatctgcatc ttcaattaat atttcaacat
    23821 cttttctact atgacacctt tttatactta ctccagcact tatagttata tagtttttta
    23881 ttttagaata tatatgttca ataccctcaa attcaagaag tttgaaaact cttttaatat
    23941 ataattctat attatcaatc tcttcaacac cgattgattc tatagaatct actaatatta
    24001 aaatttcttc tcccccatat ctgattatgt gttcggtaat atctataaat acacttctta
    24061 taatatcagt taccttaact ataactttat cccctgctag atgaccataa ttatcattat
    24121 aacttttaaa acaatctata tcgaccagta ttgcaaccat atttttgtct ctggttccat
    24181 taaataaatc caactgctgt tccaaatatc ttctattata tagtttagta agtccatctt
    24241 ttaaaatatc attttctaat ttcttattat catccactag taatcttaaa ctctcttgtt
    24301 tttgtctgag ttcatattgt ttgtgaattg cttgagatgt tttataggaa ttattttttt
    24361 cccacctatc agaataccaa tccatacttt tataatattc tattgccagt ttaaaatctt
    24421 tttttagctc atatccattt gctattagcc cataaaatat agatattttt ctaagattca
    24481 atgaactctt tttaactaaa tttaaacctt ttatataaca acttatacct tcatctatat
    24541 taccaaattt tatatataaa tctccataca acttatatat gtaatctagt ttagaattat
    24601 atataaaaac atctccaaaa cttttatata tattttttga taaaattaaa taggtgttta
    24661 ccttttcaaa actacaatta tctttcttta actcaatttc agctgaatat aaaaatataa
    24721 atgaatataa ataatcctta tatacacctt caacaacttt cattaatttt atagtcttta
    24781 ataaaataac ttctgcttct ttattcatat cttcttttaa atatgcccat ataaggtcta
    24841 tttttataga cactctttca gttactgata ttgagttatt actgtgtgca acatctaata
    24901 cattatttat gtattttata gcttctacat tatttccact ctctaaataa cacaaactta
    24961 ttatacattt acaaaaagcc tctatagagt ataaattatt ttcttgagcc aattttagac
    25021 attcaattaa gtattccata ctttttataa agttattaaa taaatataga taaactctcc
    25081 caaatatata atttgcccta cttacaagag ctttattttt taaagtttta aaaaaagaca
    25141 cttcttctac taaagctatt acatccgttt tggtctgata taaagcactt atttctacac
    25201 ataaactcat atttaacaat accaacaact catttaaatg attttgtctt ataatttttt
    25261 ttgctgagaa ataaaaatta tttaccttat catattttct tatagaaata gctgataaag
    25321 taagataaat ataaatttta gatattatat ctgaatcatc ttttaatgac aattttttaa
    25381 tatactttaa tgactctatc aaatagttgt atgatgcttc tctatctttt tttatatagt
    25441 ttataagact aattaaatat aaagtataga ctctactatt atcttctaat tttgtattta
    25501 aaaggtcttt gtgataatcc aagatgtagt ttaaatctgt caaatttaga tagaatttag
    25561 tcaacatatc gtattctatc ttgtttatat ttctctttaa atcttctatt aaactccttt
    25621 tcaagtaaat caccaatcct atttcaacag ttaaaaattc ttaaaatcat gataatgcaa
    25681 acatctattt ctaccacttt cctttgcttt atataaagct ttatcagcat tttcaattat
    25741 ttcttttata tctatactac tatttttaca tgtatcaatg ccaatactaa ctgtcaaatt
    25801 attgtttaaa aatttcttta caatcctaca taattttctt ccaaatattt tacttttttt
    25861 ataatcttta catacagata atatcagaaa ttcttctcca ccatacctta tcataagcat
    25921 gtcttttttt agatattttt ttattattat agttatattt ttaagagcaa tatctccatt
    25981 gtagtgtcca taagtatcat tgtattcttt aaagtaatct acatctatca taaatgctgt
    26041 gcaatattca ctaacttctt gtttttctaa aaaatcgttc aaaaaagttc tattatatgc
    26101 acttgtcaaa tggtcaacat actttttttt atctaaatca agttttactt ttctcatgtt
    26161 atttatttct ttttctatat actcatgtct ataatttaag gaatctatat aatttctatt
    26221 tcgagctttt atatacatca tttttaactc ataatacatt ttaaagtatt tcatactcat
    26281 ttcataattt tgaatctttt caaaacacaa atatatactc ttatatgcta acttcacata
    26341 attataattt ttagcatttt tagatttttc aaggagtttt ttatttgtgt ttaaggcatt
    26401 gtcataatat ccaaagatat aatatatctt tgcctggtga taaattattt ctaaatcata
    26461 tattatatat tcttct
//
LOCUS       DAKBNN010000030        26444 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000030 DAKBNN010000000
VERSION     DAKBNN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.030"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(141..335)
                     /locus_tag="OUB02_001143"
     CDS             complement(141..335)
                     /locus_tag="OUB02_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884915.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWVDRVRANSYSYENKIAK"
     gene            complement(644..2173)
                     /gene="lysS"
                     /locus_tag="OUB02_001144"
     CDS             complement(644..2173)
                     /gene="lysS"
                     /locus_tag="OUB02_001144"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCT8884916.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2188..2667)
                     /gene="greA"
                     /locus_tag="OUB02_001145"
     CDS             complement(2188..2667)
                     /gene="greA"
                     /locus_tag="OUB02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCT8884917.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2776..3744)
                     /gene="dusB"
                     /locus_tag="OUB02_001146"
     CDS             complement(2776..3744)
                     /gene="dusB"
                     /locus_tag="OUB02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCT8884918.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3881..4651)
                     /locus_tag="OUB02_001147"
     CDS             complement(3881..4651)
                     /locus_tag="OUB02_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HCT8884919.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4670..5260)
                     /locus_tag="OUB02_001148"
     CDS             complement(4670..5260)
                     /locus_tag="OUB02_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT8884920.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5443..6411)
                     /locus_tag="OUB02_001149"
     CDS             complement(5443..6411)
                     /locus_tag="OUB02_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HCT8884921.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKRTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6404..7381)
                     /locus_tag="OUB02_001150"
     CDS             complement(6404..7381)
                     /locus_tag="OUB02_001150"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCT8884922.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7696..9666)
                     /gene="ftsH"
                     /locus_tag="OUB02_001151"
     CDS             complement(7696..9666)
                     /gene="ftsH"
                     /locus_tag="OUB02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCT8884923.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9700..11088)
                     /gene="tilS"
                     /locus_tag="OUB02_001152"
     CDS             complement(9700..11088)
                     /gene="tilS"
                     /locus_tag="OUB02_001152"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCT8884924.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDNFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDCIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11271..11699)
                     /locus_tag="OUB02_001153"
     CDS             complement(11271..11699)
                     /locus_tag="OUB02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCT8884925.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11716..12522)
                     /locus_tag="OUB02_001154"
     CDS             complement(11716..12522)
                     /locus_tag="OUB02_001154"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCT8884926.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12724..13209)
                     /locus_tag="OUB02_001155"
     CDS             complement(12724..13209)
                     /locus_tag="OUB02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HCT8884927.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13260..13919)
                     /gene="spoIIR"
                     /locus_tag="OUB02_001156"
     CDS             complement(13260..13919)
                     /gene="spoIIR"
                     /locus_tag="OUB02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HCT8884928.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14064..14744)
                     /locus_tag="OUB02_001157"
     CDS             complement(14064..14744)
                     /locus_tag="OUB02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8884929.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14728..15618)
                     /locus_tag="OUB02_001158"
     CDS             complement(14728..15618)
                     /locus_tag="OUB02_001158"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCT8884930.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15736..17286)
                     /locus_tag="OUB02_001159"
     CDS             complement(15736..17286)
                     /locus_tag="OUB02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HCT8884931.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17380..17646)
                     /locus_tag="OUB02_001160"
     CDS             complement(17380..17646)
                     /locus_tag="OUB02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCT8884932.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17794..18654)
                     /gene="yabG"
                     /locus_tag="OUB02_001161"
     CDS             complement(17794..18654)
                     /gene="yabG"
                     /locus_tag="OUB02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HCT8884933.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18738..19676)
                     /locus_tag="OUB02_001162"
     CDS             complement(18738..19676)
                     /locus_tag="OUB02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HCT8884934.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19786..19947)
                     /locus_tag="OUB02_001163"
     CDS             complement(19786..19947)
                     /locus_tag="OUB02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884935.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19957..20454)
                     /locus_tag="OUB02_001164"
     CDS             complement(19957..20454)
                     /locus_tag="OUB02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HCT8884936.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20569..21279)
                     /locus_tag="OUB02_001165"
     CDS             complement(20569..21279)
                     /locus_tag="OUB02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HCT8884937.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21455..21985)
                     /locus_tag="OUB02_001166"
     CDS             complement(21455..21985)
                     /locus_tag="OUB02_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884938.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKPIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22002..22232)
                     /locus_tag="OUB02_001167"
     CDS             complement(22002..22232)
                     /locus_tag="OUB02_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884939.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22247..23641)
                     /locus_tag="OUB02_001168"
     CDS             complement(22247..23641)
                     /locus_tag="OUB02_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HCT8884940.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23767..25020)
                     /locus_tag="OUB02_001169"
     CDS             complement(23767..25020)
                     /locus_tag="OUB02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8884941.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25071..26225)
                     /locus_tag="OUB02_001170"
     CDS             complement(25071..26225)
                     /locus_tag="OUB02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCT8884942.1"
                     /translation="MSNNINQNLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
BASE COUNT         8097 a         5116 c         2613 g        10618 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt
       61 ggtttttttt aataaatata attaaaacta atagatataa cccttgatat tacaaacaat
      121 gcatgcatat ataattttac ctattttgca attttatttt cataagaata tgaattagct
      181 ctcactctat ctacccaaac atttatactc ttaactttat taacattagt tgtaccctca
      241 aaaatatcca ttgcatcctt acaaaaatat atcataacat caagaaccaa aaatccaaca
      301 caaaataaac ataaaaaatt aattacttga aacatcagac attcccccaa taatttttta
      361 tatatagtat tctcataaat tattcttttt accttctcat aaattattct aaatattttc
      421 ataataaatt actttcaatc atattttcta aatataaagt ctcaatcaac aaaaactaat
      481 atcaatttct aacgtttgtt ggtaatttat aaattaaata tctaaaagaa tctttgaagc
      541 atataaaaag cattctacac tgacatttta aataaaattt agctatttat tgataattta
      601 atagctaaac tttatttaaa acttagtaga atgctaatat ttcttagttt tcttcttctt
      661 tatttggatt gttatcaata ggtttcatag tcgggaataa taatacatct cttatagatg
      721 gagagttagt taataacata attactctat ctatacctat tcctagtcct cctgtaggag
      781 gtagacctac ctctaatgct tttaagaaat cttcatccat ttcaaaagct tcatcatcac
      841 ctaattctct ttttcttaat tggtctataa atcttccttt ttggtcaata gggtcattaa
      901 gttcagaaaa tgcattagct aactcccact tattagcaaa tgcctcaaat ctatcagttc
      961 ttcttgggtc ttcaacattt ctcttagata atggagatac ttcaactgga tgatgagtta
     1021 taaatgttgg ttggattaat ttatcttctc caaattcttc aaagaaagca tttattactt
     1081 cccctcttct cattccaggt tttatctcta tacctttttc cctagcaact tctagtgcct
     1141 cttcatctgt atttatagta gaaaaatcta caccagaata ttctttaaca caatcttcca
     1201 tactcattct tttccaaggt ggtgtaaagt ttatttctgt tccttgatag ttaactatca
     1261 tacttcctgt agcaacttca gccatatgtg caataacatt ttctgttatt tccatcatat
     1321 cagtatagtc tgcatatgct tgatataatt ctattgcagt atattcagga ttatgtttta
     1381 aatccatacc ttcatttcta aacattcttc ccatttcata aactttatca aatccaccaa
     1441 caataagtct ttttaaatac aattcattcg ctattcttaa atacattggt atatgtaaag
     1501 tattatgatt agttataaat ggtctagcat ttgccccacc tgctatagta tttaatattg
     1561 gagtctcaac ttctaaaaat cctctatcat ctaagtatgc tcttaatgct tttaatgctt
     1621 ttgttctaat taaaaaagca ttttttactt ctggatttac tattaagtca acatatcttt
     1681 gtctatatct tagttcttgg tcttttaatc catgatattt ttctggtaaa acttgtaaag
     1741 atttacatag taatactaca gatttagcct ttacagatat ttcacctttt ttagttttaa
     1801 agatttctcc ttcaattcca actatatctc caatatcata tgttgaaaat acactatatt
     1861 cttcctctcc tatagcatct agtcttacat aagattggat tcttccctct tggtcttgaa
     1921 tatctataaa gccagctttt ccttgtattc tcttgctcat tatacgtcct gctatttttg
     1981 tagttttacc ttcaagagaa tcaaagttat cttttatatc catagaataa tgagttctat
     2041 catatctact ttctttaaaa ggatctctac ctgactcttg taattttttt agtttatctc
     2101 ttcttacttg aagtacttca ctaaggtctt cttgaatttg cgcttcttca ttgctttgtt
     2161 gattattttt catttttttc ctccaaatta tcttcttatt tctaatattt caaacttagc
     2221 aactccatca ggaacttgca cttccacaac ttctttcgca gctctaccta aaagtgcttt
     2281 ccctacagga gattcgtttg atattttacc atcatatggg tctgcttctg tcgaaccaac
     2341 tatagtatat tcaacctctt catcaaattc taaatcctta actttaacta ttgatcctat
     2401 tgtaacaaca ttaaggtcta ttttgctttc atctatgata acagcttttc taaccatatt
     2461 ttctaatttt aaaattcttt cttcaacttg tgcttgttcc tttttagctt catcatactc
     2521 agcattttca gataaatcac caaaagatat agcaactttt attctctctg caacttcttt
     2581 tctctttaca acctttaagt tttctaattc ttcttctaat ttatcatatc cctcttgtgt
     2641 caataaaaac tctttatttt cttccattat aaatacaact ccttcacaaa tttcttagga
     2701 attttcctaa ttatatttta aaaattttat aatctttttt taatttatgc gctatattat
     2761 aagtcactat tatttttatg tcaaggagtc ttccatatag tactccaatt tcgacacaac
     2821 ttcttgatag tcttctattt tgttaatttc atcccttaat tttgcagagt ttctaagtcc
     2881 ttttaaatac caagcgatgt gttttctcat ttcccttaca gctacatact ctccatgttc
     2941 ttctactgca agttttaaat gcttaatggc agtacttatt ttttcgttaa gagttggctc
     3001 tggcaaaatc tcgcctgtat tcatataatg gtttattctt ttaaatatcc aagggttacc
     3061 ttgagcaccc ctacctatca ttatagcatc acaaccagtt ttgtctaaca tatttattga
     3121 atcttctatt gtaaatacat caccatttcc tataacaggt atactcaaat ttttcttaat
     3181 ctctgcaatt atatcccagt ctgctttgcc tgtataaaac tgttctctag ttcttccatg
     3241 tattgctaaa gcacttattc cacaatcctc tgctatcttt gctatttcaa cagcatttac
     3301 actattatca tcccagcctt ttcgaatctt taaagtcact ggcttttttg agttcttcac
     3361 tactgccctc aatacctctt ctgcaagctt aggatttttc ataagtgctg agccatctcc
     3421 atttttaact acttttggag ctggacaccc catatttata tcgagaatct catttgaata
     3481 atcattcatt atctctgctg ctcttcccat aaactctggt tctgaaccga atatctgaac
     3541 tgctacagga tgttcttcct cttctatctt aagcattttt ttagtatttt catcatcata
     3601 gcaaagtgcc ttaccattta tcatttctgt atataaaagc ccacatccct gttctttaca
     3661 aattaatcta aatggaaggt ctgttacacc tgccataggt gatagaaaaa ctttattttt
     3721 caattctaaa tttcctattt tcattgtttt tctcctattt ttgcctttat tttgtgattt
     3781 aactttaaat tcttatttcc tcattataat ataccacata tagctagaaa tctatcataa
     3841 aaagggaatt ggatatataa cccaattccc tgtatattaa ttattcttgg tttttttcat
     3901 atattattct caatccttct agtgttaaaa aaccatctac ataatctata cttttcgtct
     3961 ctgaagcaat cagtttagct aatccacctg tagctataac cttaacatca tcacaattca
     4021 attctttctt catcatactt attattttgt caactaaacc aacatatcca taaattattc
     4081 cagattgcat ggctgataca gtactcttac aaatagtcat acctggctta gctaattcta
     4141 ctctaggtaa tttagacgca ctttgaaata acgcctcact agatattttt attcctggtg
     4201 ctattgttcc acccaaatat tcaccttttt cagagatagc acaaaatgta gttgctgttc
     4261 caaaatcaac aagtatactt ggtgctccat acttttctat ccctgctaca gcattaacta
     4321 ttctatctgc cccaacttgt tttggattat catattttat atttagacct gtttttatgc
     4381 ctggacctac tattaatggc tgttttttac agtactttat acaaaagttt tcaagagaat
     4441 gcataacatt cggaactaca gatgatatta taacatcatc aatatcactt atattcacat
     4501 tatcataatc aaataggtta cttatcagga ttccatattc atcagacgtt ttttccctat
     4561 ctgttttaat tctccagtaa ttaactaatt tgtcaccttt atatatacct aaaaccatat
     4621 tagtatttcc aacatcaaat actagaagca tatgtcatta accttctttc tattttttgc
     4681 ttttcttaag tacagtggca acagcagcta ctactaaagc ccctactatt gcttctggca
     4741 ccccatttgc tgcggcaaga gccattatta cttttcctgc tgaaccactt ttacccattg
     4801 catgaagata cttgtcagca tataagacat atatcattcc taaaactcca gctgtattta
     4861 acagagaacc tattgcccct gtaattaaac ctgatatgta taccttattt gtagccttaa
     4921 ttattaactg atatacataa taagctaata cacctatcat aactcttggt aatattgaaa
     4981 ctaatggatt tataaaaaca aaagatgtta ctgttgggtt tgttatagca tttaagagac
     5041 ttgatatccc aaatataaat cctattgctg ccccaactat tggtccttcc ataatagctc
     5101 caataataac tggtatgtgc attatagttg cttttgtagg tccaactgga ataaatccca
     5161 atggagtcat agatagcata atagaaatag ctgatagtac tcctattata gtcatctttc
     5221 ttacatttac ttttcttact gatgcacttg tgttcatcat aaaaaataca cctccgttcc
     5281 agctcattaa gatgccggac tacttaattt tttaattttt tcgttttttt cttataatta
     5341 tgagtcaagc taccattgta ggatgcccgc tctaacaaga ataattttat caagatgagt
     5401 tgccattgtc aacaatttga caagaaaaat atgctaattt catcatattt ctcgtttttt
     5461 tactatatct ttatatgcca ttatattttc tacactatcc gcttttttta ctgcatttat
     5521 tatacttttt tccacaacct ctacagccaa tgaacctact aatgttatat ctgcttctat
     5581 ttctcctgtt gatattgtga atattgtatc tccatcatga ggtgtatgga taggaaaaat
     5641 agacttagca tatccatcat gtcccatttg agatatcttt ttacatccag ctttggttaa
     5701 cttagcattt gttgcaatta tcccaattgt agtattatct acactaaatc cacctttact
     5761 ttcaccttgt ttcattaatt tatatgtatc aattgtttct gtccttttat catttaaagc
     5821 accagctata accttaccat tttcataaac atctccaaga gcatttacag caattaatgc
     5881 agatacaact agaccattat ctaattttat agaatagcta cctattccgc ctttcatagc
     5941 atattcctga cctcttatct ttccaacagt ggctccacaa cctgctccat aatttccttg
     6001 atttaaaata tcagtatttg caacttcaca agctctataa cccatcttta aatctggtct
     6061 acatttaaag ctccctactc ctaaatcaaa taaaactgct cctacaacta taggcacttt
     6121 acctacacct acatcaaagc caattccttt ttcttctaag tatctactta caccacaagt
     6181 ggattcaaga ccatatgcag aacctccagc caaaactact gcatgaactt tttgaaccat
     6241 atttacaggg tctaataaat cggtttctct agtccctgga gcagagcccc ttacatcaac
     6301 accgcaagta gctccttctt cacatattac tactgtgcac ccagtaagac catttctatc
     6361 ctcaacctgc ccaaccttta agccttctat atccaaaata ttattataca tatccttttt
     6421 ctcctcttat agaaacttca cctgaaataa tttcttttat acttccatca cttctttgaa
     6481 ctaataaatt tccctcttca tttatgtcta aacatttcac tagttctcta ttatctcctt
     6541 ttaatacata tatattttta tctataattg cagaataatc tctacatatt tttaaggttt
     6601 tttctttgga attatttaca gtataatcta aatataattt ttcaaattca gtcaatacat
     6661 tcctaacaat atctactctg gataacatat acccttcttt atacaatgat gtggctttgt
     6721 cagctatttc ttgttcaaaa tccattattt taacatttat tccaattcca agaacaacat
     6781 aatttgttct ttcaatttca gcagataact ctgttaatat accacatatt ttttttccat
     6841 ttaaaataac atcatttggc cattttatta atgtatttgc tcctagattg tttaaagctt
     6901 tcgttacact tgctcctgct attaaagtta taaatggagc tttgaatggt tccatatttg
     6961 gttttaatat aatactcatc cagattcctt catgaggttt tgaatcccat cttcttccca
     7021 atcgtccttt ccccatgtct tgatgttcac ttaaaatcac agtcccatct acactattta
     7081 tagctatcct tttagcatgt tcatttgttg aatctatagt gtcaagatgt tcaatatttt
     7141 ttccaataaa ctctgtatct aatttatagt aaatattctg tgggcttaat aaatcagttg
     7201 gattttccgc taatcgatac ccttttttat ttacagattc tatattatat ccctcttctt
     7261 ttaaggcttt catatgtttc catatagctg cacgtgacac ccctaattgt tttgataatt
     7321 cttctcctga aataaattca gatttattat ttaatataat ctctattatt ttatctcgca
     7381 tatttcacct ctaatatgac ataatttcat gacttatttt cttctaataa ataattatac
     7441 attattaata tacttttaat actattaatt ttaaatttcc tttttaatta ttataatggt
     7501 tgttttatat tttatattga tgatttatat aattttacat tttatttaaa taattttaaa
     7561 ataataaaaa aactgaaagt atcaattaaa ttaatacatg caaatataat ttgcttatta
     7621 atttaatatt atactttcag ttttagtatt taatccacat cagataataa tcttaaaatg
     7681 accaaggtct taaaactatt tatctttatc agatttatct tctaaatttt tatttatatc
     7741 aataacattg tttctttctt cttcatcttt ttctaatttt atagttaatt gttcttcaac
     7801 ttctttagaa gaattttcct ctgtaactgc attttcaatt tcattcagag gtaactcttt
     7861 attaaatgct tttacaaatt gttctgcatc taaagtttca tatataagta aagcttgtgc
     7921 tacatactct aatctatcca tattttcttg taatatactt cttgtcttat catatgcacc
     7981 atcaactata cgctttgttt cttggtctat ctcaaaagca acttcttcag aataatttct
     8041 cttacttgaa aaactattac ctaggaatac ttcatcatca ctgtcaaatg acattggacc
     8101 aagcttagaa ctcattccat actttgttac catacttctt gcagtagctg taactctttc
     8161 taagtcattt gaagcacctg tagatacatc cttaagtact agctcttctg ctactctacc
     8221 accaagtaaa actacaatat tttctttcat ttcatttttt gtagcataga acttatcttc
     8281 aacaggaagt tgcattgtaa accctccagc tcttcctctt ggaactattg taacttgatg
     8341 aacaggactt acttcttcaa gtacatgggc acaaactgca tgccctcctt catggtatgc
     8401 tgtaagtctt ctttctttct cacttattac tttcgatttt ttagccacac ctgctataac
     8461 ctttgttata gcttcttcta tagtttccat cttaattttc ttttctcttt ttctagctgt
     8521 taatattgca gcctcattca ttaagttctc tatatcagca ggagtaaatc ctggtgttct
     8581 tctagccaag acatccattt ttacatcatc acttaatggt ttatttcttg agtgaacttt
     8641 aaatatagcc tctctacctt ttacatctgg tgtaccaaca acaacttgtc tgtcaaatct
     8701 tcctggtcta agtaaagctg ggtcaagtat atctggtcta tttgtagctg ccatgattat
     8761 tataccttga tttaccccaa atccatccat ttcaactaga agttgattaa gagtttgctc
     8821 tctttcatca tgacctccac caagacctgc acctcttttt ctaccaactg catcaatttc
     8881 atctatgaat atgatagctg gagcactttt cttagcctgt tcaaataaat ctcttactct
     8941 tgaagcacca accccaacaa acatctcaac gaaatcagaa ccacttatac tgaaaaaggg
     9001 aactcctgct tctcctgcaa ctgctcttga tagatatgtt ttaccagttc caggaggtcc
     9061 taccataagc attccttttg gtattcttgc acccaattca atatatttct taggattctt
     9121 taagaaatct actacctcct gcaaatcttc tttttcctca tctagtccag ctacatcttt
     9181 aaatgtaact ctagtttttt catcatcttt atgaacctta gcttttgatt tgccaaaact
     9241 cataaccttt ccgcctccac cttgagactg attcataaac acgaaccaaa taatcaccat
     9301 gaagaatatt aacagtaatg taggcaacat ttcgacaaac caaggtgtag atggcttagc
     9361 ttcaccacca aaagttagtt tcccagcctt agcttggtct aacacctcat ctgcaagttt
     9421 atttcccatc acttctgttg gaacatatga cttaaacttt gtatttgtat cttttatagt
     9481 accttctaca gaagtttgat ttacaaagta taatcttgat atattctcat ctgttaattc
     9541 tctatatact tcagagaatt ttaaatcttt tattttttct gtaggcttac ctgagaattg
     9601 aactatacct actataatta taaaaacaag tagataaaaa cctgcccctt ttagcaactt
     9661 attcaatata gaagccctcc tttcatacat tgctttgccc tataatttgt taaaattaat
     9721 ttttaatact tcttttgtgt tttcatcaat tttataattt tcactgattt tataatctcc
     9781 aacacatatt attccttcac tatctgtgat tataggaatt ttacatcttt cttctcttgg
     9841 gatttttaaa tctatgaata aatctttcac ttttttactt cccatagcaa gctttatctt
     9901 atcaccttgc cttctgcttc ttattactat accccctttt accttattaa aatcaaaccc
     9961 tttagatgaa ttgtctaatt tcatactctt atacctatct atgctcatta cttgggtctc
    10021 aataatttgt tttaattctt ttattttaat aaacccatta ctaggtacat tgtaataaaa
    10081 ttcaatttcc tcattaacaa tttcctcatt cgttaatatt atacagtctt tttttctata
    10141 aacaaatata tttcttggaa gagttaacat tttatttact tttgaattat cttctaaaga
    10201 cattatatcc tctatatgtc tttggtcaac aaaattagta tctcctagta tatgttttat
    10261 ggaatttcta agaactctaa ctttgatagc atcatgtaag caaacaaaat tgtctaaatt
    10321 tatctctaca aagccttttt cttttatatt cgaaacttct ctaaatttag cttctgcctc
    10381 tttctcaata taatcgttat cactttttaa actattgctc atcctcacta tagattctat
    10441 tacatttgaa ttaaaattat ccttcatata tggtaaaagg tctaacctta tcttatttct
    10501 agtgtatata ttttctaaat ttgttttatc tatcctcgga tttaaattat aagcttcaca
    10561 atactcctct atctcatttc tttccacgtc taatatgggt ctaattatgc aattatctct
    10621 aatataatca attcccttta aaccttttaa acctgttcct ctcatgatac gcatcataac
    10681 cgtttcggct tggtcattga gattatgacc tattgctatt ttattggctt taatcttatc
    10741 cttaatttca taaaacattt catatcttag cttccttgct ccttcttcta atgataatcc
    10801 ctcattttca caatattttg gtacattaat agactttaca aagaatataa ttcccatatc
    10861 ctcacaaagt ttagatacat ataatgcatc tttttgagct tctatccctc ttatttgatg
    10921 gtttaaatgt gctgcatata tttctatatt aaaatctttt tttaatctat ttagtacatg
    10981 caataaacaa actgaatcag gtcctccaga aagacctaaa actattttat cgcctttttg
    11041 tattaaatta tgtttattta tagtacttag taccttatca aatatcatat ttatccctct
    11101 tacacttagt tttattcact tttaagtata ctttctataa aaaaacgcta aacccttgta
    11161 tcaattttaa gaaattaaag tttaatataa atataataat accaaataaa ttaaataaaa
    11221 ggtagttttt tcactacctt tataaaattt ctggaaaata gccatagtta ttatttagta
    11281 cctatgtata tatctatttt attcctacct aaaaataaat cttgtacctt tatattatag
    11341 tctattttca attttatgtg ttccccttct tgtatattta tatctaattc tcttgtatca
    11401 atatcaatta ataaagtacc ttgtggggtc acatactttg tagtactact ttctccacat
    11461 ttaaacagca ttgttgagtt aatagcacca aactttttga tactaacttg atttttaagt
    11521 atctttatag tattagttat ttctatattt tcttcttttt ccttgtaaat aacatatatt
    11581 ccatcatttt tataaaaaat tttaccaaag gcagtcatct ctatggtgtc catatttcct
    11641 ttttcatcat attgtctagt atttatactt atttttacac tcaacgattt ttcctccata
    11701 gattcttcat ataaactagt atttttgtat ttctactttt tgaatcttat caattttttt
    11761 ctttaagaac tctcttgcta tatctgaaaa tttttcaggt atatctgata cataatattg
    11821 ataagttcca tgaatttctg tattgtttat aatattttgc acctctaaaa tcttttttaa
    11881 atcctttgca gtctctttag ctggattaac aagttttata tgctctccaa cttcttcacc
    11941 tattgttctc tttaaaatag gataatgagt acatcctaac actaaagaat ctatattttt
    12001 ttctttcaat tcttgtaaat atatctttgc tgtcaattta gccacttctg tatttgccca
    12061 tccttcttca acaataggta caaataaagg acatgcttta ttaacaattt ctatactctc
    12121 atctatttta gatatttcca agttgtatgc ttttgaactt atagttcctt cagttcctat
    12181 tattcctaca attttgtttt ttgtagaact tacagctgtt ctagctcctg cttctataac
    12241 tccaattatc ggtatatcat atttttcttg agcttctttt aagcttcttg cagtagcagt
    12301 gttacatgct ataactatag cttttacacc ttttgttttt aaaaagttta tagcttgaaa
    12361 agtatattta attatagttt ctttggatct tgaaccatat ggtattctgg cagtatctcc
    12421 aaagtatatt atatcctcat ttggtaatat cttcattatc tcttttagaa ctgttaatcc
    12481 acctaatccc gaatcaaaaa ctcctattgg tttattgttc atctttatac aacacctact
    12541 ttacattttt gtgtttaaca acaacttatt tatcgctaaa atttatattt atattcataa
    12601 ttaatatatt attacctaaa tgattattat agtttatttt acacaaaaaa taaaggagct
    12661 atcttaaatt agaaaataat ttctacaaaa agacaactcc tttcactact taaaaattta
    12721 aatttatttt gcaaacacat gatttcctat tctcatataa ggattcaatc tatttatcca
    12781 tttgcaagtg gctatttctg gattccaaaa atatactgct ttattagttg ggtcagttcc
    12841 ataaagagct tcctgagctg ctttacgtgc tgagtctgtt ggtgccatct ttattgaacc
    12901 atcaagcaca actgagaaag catttttttg atatatgact tcttctattg tgtctggaaa
    12961 tctagggtcc aatactctat ttattactac agctgctaca gcaacctgtc cctcatagct
    13021 ttctcctcta gcttctcctg caaccaactt agataataac atcaaatcat tatcagataa
    13081 atttattact tctttctttg ctatattttt ttcttgtttg tttactttct taatattttc
    13141 tgttggttca tatgcaaata cctctttaat aggcataaat ataaaagtca aagctaatag
    13201 tgtagccatc agtttactcc acctgtccat gattaaaacc actccttaca ttcttatttt
    13261 taaaattttt tctctaaatc cttgactact tcaagtactt taaacttaat ttttactcta
    13321 cttgtttttt ttggtgtatc ttgttttatt aacttatatt ctttatcagt taaaacttcc
    13381 ttcaacttat catctgtaga tttatctatc actccattag attcatcaac aaaacatagt
    13441 ggagggaaca taacacacca ccagtttttt ccttcacctt tcccaatgat aatctttaat
    13501 gctttatatt caccagctgg aagaactata ttagaatatt gtttagtagg gaaattgcta
    13561 tattgtatac ctacctttac actgtaatta tatccatttt taagaataat atttttactt
    13621 atttcttcca gactactata ttcctttttt ataatagctt cactttcttt tatacttttt
    13681 gaattttgaa gctttggctg taaatatttt attacttcat ctctaacttt tagtttaagt
    13741 tcttggtctt catctgtatt gctgtttgct ataacatgaa atcttattaa tttatcttta
    13801 taatcttttg atatattgtc tacttttttt acctcaccat ttattactat tgtcattatt
    13861 gatatgactg atataagact taatattaat agtcccaatc taatcttaat ttttctcatt
    13921 gaaaattccc cctcaagata aatttatatc atcaattatg tccaattaaa tttaatttat
    13981 tcataatatt ttttaatact aataaaaaaa gctatcttaa aatgattaca atcattttaa
    14041 aatagctttt taaaccttat attttataag tctttactaa tcatagcctt atctatcata
    14101 aactgctctg ctttttctat atgctctgta gcatacttct tggatagctc actatcacct
    14161 ttaattatag catcaagtaa atatttatgc tcttcaacaa tattatgtga tgtatcataa
    14221 gcagacatat aggtaactct aaatctatat acttgttccc acaatgagtt tattaattgg
    14281 attaattttt tattattagt tgctttaaat atacattcat gaaattctac atcaagtttt
    14341 aataacgcat ctacatcgga ttccaaagtg ctttttttaa attcttctga tatatcttta
    14401 agctttttaa tatcttcatc actaattctt tcagcggcaa gatatgctgc taagccttct
    14461 aggcttgctc ttacttctaa tacatccata atatccttta atgacatatt tgccacatac
    14521 gcaccttttc taggcaacat aacgactaaa ccttctaatt caagctttct tattgcctct
    14581 ctcactggag tcctacttac tcctaattgt tctgctagtt gcacttccat tagcctttga
    14641 ccaggcttta attttccttc taagattgct tctcttaaat tctcaaagac tacatctctt
    14701 aatggcttgt aattatccaa gttcagttta gttaaattct ccacaaattt caaccccttt
    14761 ttgactgcta ttaacaacat acacctgttt atattttttt aataattctt tttttccaat
    14821 tagagcatct tctttatttt taaatagtcc aaaaactgta ggaccacttc cactcatcat
    14881 agaacctaaa gcattatttt tcatcattac ttgttttata tcacttattt ctttgtgttt
    14941 acctatagta acattttcaa gaatatttac catgctttct gatacagctt ttatatcttc
    15001 agattttaaa cactctatca aatatttatt atttggtctt tttttaatat tattcaaatc
    15061 taatccttga taaacttctt tagtagaaac gaataagtct ggtttgcata tcaatatatt
    15121 tgtatcacat ggtaaacctt tgatgtttgt tagcttttct ccaatacctt gagctaaagc
    15181 aggtcttcct gatatacaaa aaggaacatc tgcgcccaaa ttaagtccta tttcttttaa
    15241 ttcatcttca gataatctaa gttcccatag gtgatttaaa cctactaata ctgcggctgc
    15301 attagaactt cctccagcca tccctgctgc tacaggaata tttttttcta tgaatatttc
    15361 tacacctttt tttatataaa atttattttt aagtattttg gcagctttat aaacaatatt
    15421 atcttcatct aaaggaatat ctaaactagt gctttttacc tttatttcat cttcgtctaa
    15481 ttcttttatc tttactatat cgtataaatc tatcgtttgc attatcattt caacaaaatg
    15541 ataaccgtct tgcctttttc ccaagacatc aatggataaa tttatttttg ctctactctt
    15601 tagtcttata aaatccattc tatcctcctt gtttaaagaa taatgcatca ctaacaataa
    15661 tattatacta caaaaaataa aataataggg tattataata ccctattata cttaattact
    15721 ttaaattttt taatattaat ctactaatac gacttttttt tctaaaataa gacacttacc
    15781 aggcttaatt ggttcatcaa gttttatatc attaagttct gcaatttcat tttctgttgt
    15841 attgtatttt tttgctatat tccagaaagt atctccttct ttgcatatat atacgattat
    15901 gctaggagct ttagataaat catatacacc ttggtcttct cccttgataa taaaattttc
    15961 tgctttctta tctaatgctt cagtaaatct cttaattttg ataatcaggt ctatctggtc
    16021 cctattcaaa tcaacttcga ctttatcaat acatgctgta ttaaacacag atgctgtatc
    16081 agttagatta tccatttcta tatcatgttc aaatggaatt tcctcactaa ttttataaac
    16141 caattttaaa ccttcaactg ggacaaataa aatttcaact tttatgattc cttgtataat
    16201 gcttttattt ccttcaatat aactattttc tatagacatg gttggacaaa cgcttacaat
    16261 atcttttatt tgtatatcat cattatcatt tcttatgcct tctctaacca taaatgtttc
    16321 ttcactacgt cttaaagtct tatttaattg aataggtttg tggtcaaatt ttattatctt
    16381 ttgaggcgaa tatgcatctt gtaatacttc tcttgtaact tcatctgtaa cttttacctt
    16441 acaacaagcc atacaatcta tttctaaaag acctgtgttg ctttcactat tttgcttgaa
    16501 aatatgatta aaatctgaca tagacaacaa tacttcttca gtcataccat cactaactcc
    16561 agggacttct acaaattgag taaactcaat acctactcta tccaactcta caagttcacc
    16621 ctcatatgta caagctaacg gatttatttc taatactcca ccaataataa ctttattgtc
    16681 agtcactcta ctttctttta ctttaacaca tggatttaaa cttattatag attgaatttc
    16741 ctctgtgttt attgttatag tatctcttat agaactttct gctttttcta ttccaactat
    16801 atcttgaaaa caaatttcct ttctgtgctt ttgtatgcct tccacttgcg ccacatcttt
    16861 tacaatgtct aacctttgct tttcaaacaa acttcccctt atattcataa gagctcctac
    16921 tttaatcttt ctctcattca ttatagtaca atccatatgc tctacttctg aaaataacat
    16981 gtattccata tcttgaataa cattgtcttt ttctattact tcgtttatat ctacttttcc
    17041 gtctacatta gaaatagtat ttttatcatc tgcaatatag attacattat aattaaaact
    17101 acctctgcaa agtatcttcc catctgccat ttcaattttt ttaagtgata tgtatccttc
    17161 tgtttttaca atttcatata catctaattt tttgtctggt acaactgcct ccgcttcaat
    17221 gaatgtttga aattttccaa agtctattct attgtcaact ttaattacat ctttaattaa
    17281 ttccatagtc tacctcctta agtattataa aaatatcaca ctatatttat atttaaggag
    17341 caaaaaaaaa attacaccta tttttaaata ggtataattt taacttattt gtaatttatc
    17401 ttgatctcta aaaacttgca actttacatt agatgttaat aaatctgagt aactataaga
    17461 tactctagga tatccattac tttcatcatc taactttata acaaacacgc ttggataaac
    17521 tttttctagt ataccctctt ttgtaataat ctgctttctt cctttattag ctttcaacaa
    17581 tattttcttt cctatatgtc tctctaagct tactcttatc ttatctagag tttgaacagt
    17641 agccacaata tcaccttctt taacgtttat atgtttacat aatatcacat ataaacattt
    17701 ttgtcaaatg tttaaactaa ttattttata tattttttgc aaaaatgtca atactatttt
    17761 tgaagatata cagtataatt atgttaattt ttgctaatgt aatattgttt ttggcattgc
    17821 ccatctaaat tttcctcttg tttcagaacc accaattccc ttaacacctg taatagtctg
    17881 ctctataacc tcatcaatag gaactactac atcaattctt gaacaagcaa ttttctccac
    17941 tataaaaaca gggtctaatg catgtatcat tattcttcca ggcgaacttg catagtttgc
    18001 tcctgcactt attatttttt cataatttga ttggcatgct cctgcgaata ttacaagatt
    18061 atccaaattt ggttgccatt tacgtgcttc ttttactgtt ttaacaaaat ttgaagagtt
    18121 tctatagtta ctcatatctt ctatatctcc tctttttatt gtcattgcat catgacctgt
    18181 tattactagt atatccggat tatatttttc tagtagtgct cttacttctt tatactgatt
    18241 ttgctctgaa acagctacac ctactgcagg tattccaagt tgagcatata catccaagca
    18301 aatttttaaa tattctttat ctccatctat ctgtagtact tttccaggca ttccatatgt
    18361 gttaggattt aattgaagtt ttggaactgc tctagtcatt cttttttgtc tttctttagc
    18421 ttttcttact gacttatata ataaattttc tacattttta tctattaata tatcttttat
    18481 atcaggtgct tttactaatt ctaggtcatc aatataagca tctgctatta ctctaaatgc
    18541 aattcccttt agtatagcta ttttctcatt attttcatct acaccaaaag agactatttt
    18601 aaatactata tctttgttat gtgactttct agctacaatg tctcctacct tcatagttaa
    18661 acctccaata aatttttatc ttagtttata ctatgttcga tataaaactt tgtttcctaa
    18721 tgtcaaaaaa acatttctta taatacttct tttatcactc ttaaagcatt tttatagtat
    18781 atcttttcta tatcatcttc actaaaccct tcttttttta gaggttcata cagtttaccc
    18841 atctgagata tatcttcaat ctcaactttt gaatctattc catcaaaatc tgaccctaga
    18901 gatacaacat ctattcctcc aacatttaca atatgtttta tgtgtctaac catatcttca
    18961 agtttacttt cacttttatc acttaaaaat aagtggaaga agtttatacc tgtaactcca
    19021 cctttatttg ctaatacttt aatcatatca tcagttaaat ttctagaatg attcatcatt
    19081 gctcttgaat tagaatgtgt agcaattatt ggtttagaag aaatctttgc aatttcataa
    19141 aatccaccat ctgaaatatg agaaacatca accaacattc ccaattcatt catcttatgt
    19201 accacttctc ttccaaaatc agtaagccct ttttctctat attctacttt attatgagga
    19261 aagctcaatt cattcacatg attccaactt attgtcatca ttctaacccc taagtcgtaa
    19321 aatttcttta aattttctaa tttaccttca agtactgcac cttcctcaat agataaaaga
    19381 gctgtaagtt taccttctga ttggtttttc ataatttctt catagttagt agctaaagct
    19441 atttcatcac tatttttttt catttcctca tgaaatttat ttgccatact catgcaataa
    19501 tcaaaaggat gttttacttc ttcagtatca acaaaaagag caaatgtttg agctaatgaa
    19561 tccccctttt tcaatctgtc tatatcaaca gaatatttat tagacttaag ttcacttgtt
    19621 tctacatttt ccattaattt tgctatagta tcacaatgta aatcaagaaa tttcatattt
    19681 ataccccctg tatattttat atttatatct aaaaatagaa atagttgtcc ttatagacaa
    19741 ctattgacaa ctatttttta atattctttt taatattact taatcttatt cttcttttaa
    19801 gttaggtatt acttttttaa cttcttcaat ttgatgccat gttttatcta tattattttc
    19861 actaatatat ttttttaaat cagttaaaga tgtaaaatct ttaactgata aattgtcatc
    19921 atcatctgca aatcttaaaa taaacatgag actcccttac tttccacaac atttcttata
    19981 tttttttccg cttccacatg ggcatgaatc atttcttcct atttttgctt ctttaactat
    20041 tgttttagac ttcttataag cctttattat ttcttttctt tggtctgctg gtagtatatc
    20101 ttcccaacct tgtaaattat atagccaatg agcctcaaca gttaccatat tgttatataa
    20161 tttttcccaa tctattttta tagaaacttc tttatcagct tctacttctt ctaaaactat
    20221 atcttctttt aagctttcac ttattccatc tatgaatccc atgaaaaatt cattagtagt
    20281 tccaaatttt tcagccaatt cacttacttt accttcaaca acatccattt tattatttaa
    20341 tatttcattg tatattccag cttcaacttt caaatattct tcccagaatt ttacttctgc
    20401 ttcttgactt tcatgattat cacttaaatt tctccattca gtatataaac tcatactttt
    20461 tcctcctaag tatacctata tttttatagt ttttatagta taaaatcttt tagttagatt
    20521 ggaagtttct tctttttgat tataccatat tctatttaaa gttaactctt atattatatc
    20581 ataattaaac aattctttta atacttttat agcatactct tggtttataa aataagggct
    20641 tgtcgcaatt gttattactt tggctttttt aattaagttt tttatcttgt aaaatttgag
    20701 ttcaaatggt atatagtcca tatcacttga aaatatatcc aaatctatat caagaacaaa
    20761 ctctgattct acttctaaat caaatccata tagactatct attattatta attctgaaaa
    20821 tatgttatat tttaaagcag gttttataaa acttcctaca tttaaaactt catttgtata
    20881 tctaaataca tcttttagat tatttacatc tacatcatag ttctcaggtt ctctagtatc
    20941 cttatgttgg tctacatgca ctaacttgca tccttttgta aactcaccct ttaacaaaga
    21001 ttttgcccaa aaataaaatg catggttatg attgtcaaac acatatatct ttttatttcc
    21061 caggacatat tcgaccatat tctccaatcc ttttgctttt atttccttat tttcatctac
    21121 ttcattaaat actacctcat tgcctaacct tacatcattt aatgtaccat ttattagttt
    21181 tggaacgtat atgctcttat tttctctttt atcatatgaa aatacattat ttcctactgg
    21241 tttttctata taaaatccac tatattcata attttccata aaactttatt tcctccattg
    21301 ttatataaat atataagtta taattttaaa aattaaaatg cacttattgt aaatcatatc
    21361 atctattata gaaaacatct tatataacta aatataatat cttctctcaa tatgtattat
    21421 acaataagtg caaaatatat ttatataaaa tatactacat tcctataaca aaaccatcag
    21481 cttcaattgg aaggtaacta actcttctat tcaactccac ctttccaaaa aatactaccc
    21541 aatatggtct aaaaatactt tcaattttaa ctatttcaat ctctgggatt cctcccataa
    21601 acctatgagt taatctcata gccattttat ttgcctttgc ttttactctt cgttcgtcct
    21661 tatcactata ctgcacaata ttacaatcca cattttttac aatttcatat gtaggaaaat
    21721 cttgtactat actaccagaa cctgtagaac cattcactaa aatttttaat tcatttatat
    21781 cattagaata tctcctattc ttgaaaatgg gtttcgtttt gtgaatttta agtgtgataa
    21841 ttttaaactc tatataatga agtctaactt catgagcata tttgttcttt aatattagct
    21901 tactaaataa agaatttttt ccacttttag ctatctctat agcttcttct tttgtaattc
    21961 ttataggtaa cgctcttata tccataaaat cattctcttt cttatttttt tcttgtcatt
    22021 tttaaaactc tatatatata atcaaaaaat attacaatta caactgacat aactccatat
    22081 ggaattattt ttccaactat aatatcattt ctagtcaata taccataaaa tataaatcct
    22141 gttattaaaa agtacaataa tactagaaaa ccgtctattc caagccattt tatttctaaa
    22201 tcttttttta ccttagacac taatttagac atagcagttt ctccttttat agattatttt
    22261 gtgtatttaa attttcacct attctattac caatcatcca tgcaataaat gaaaaaggta
    22321 tagctatcac aagaggctct aactcaaagc tctgaactgg aaatatttta aaataaccaa
    22381 aagtcactcc accaacaatc attcctaaaa taccacccat agatgttctt cttccatttc
    22441 caagaagccc acctactata ggtacaaatg ccgaacccat ccaaatactt cctacaaata
    22501 tacatgcatc atatacattt tcaaatctaa atgctacgct aagccccata actgctgcta
    22561 aacatacacc tattctagta aactttacta attgtttctg agataactct ttttttgtaa
    22621 ttcttccata tatatcattt gctaatgtag ccccagctgc tagataatag ctatccaatg
    22681 ttgatattat agaacctaaa attcctatta taaatattac tcttattcct tgtggtaatg
    22741 tatctaaaac taaagttata tatccagcag ctggagccat ttctggataa gtagctctta
    22801 ctataagtcc tgtcatagtt aacaccatgt caaaacatat ccatagtgaa aaacaggtta
    22861 ataaggctct tcttccaacc ttaggagaat ctgctgctga aaatctttga taaaaagttg
    22921 ggtctgaata agctccaaaa gaatatacaa ttaacatcaa tgctttcata gctgacatac
    22981 cttttgtagg gtgcataagc tgtggtgcat ctttgagtgc ttctgtaaga ccttgaaacc
    23041 ctcctatttg ttcaaattga cctggcataa ccatagttaa accaaatacc atacaaaaaa
    23101 acaataccat atctgtaatg gcaactccca taagcccacc aagtactgta agtacaacga
    23161 ctactcctac cattattaat cctattaatt ctttatctat tttccatgct acgttaccta
    23221 tagttcccat agcagttatt tctccaagtt gtgcacaata tataaacatc aaaattgccc
    23281 caattatagc aacatgcttg ccataaaaac tttccagtaa atcaggaact gttatattgg
    23341 ttcttatatg aatccttgga ccaacccaca atgctaaagg cattctccca atatgtgcag
    23401 ctattgacca aactgcccat gcagatattc catatatagc cgcatactct acactaccta
    23461 ctgtaccaac tcctccatac catgatgcaa ccaaagttcc tactattaag gaccatggta
    23521 aacttcgatt tcctaggtaa taactttcca tatcttttac atttctagaa aaataataac
    23581 ctattcctaa taatataaat gcaaatataa ctataatccc attttcccaa aatgttaaca
    23641 tatctaatct ccacattctt atattttaat ttttaacttt aatatctaaa tatgaacaac
    23701 tctacccatt tccataaaaa taggtagagt tatataataa ttataagtat ttaagtaatc
    23761 ttgttattaa gcaattttat tgtttaaacc tttattttct cttttattta ttttatttaa
    23821 agcataagta gcaaatatcc ctataactgc acatactaca gataaaataa atatatatgt
    23881 atatcctgtt tgtccatatt tatctaacca attacctgca atagtgtaat aaaacgcatc
    23941 tggagaaaat cctataaatg aagcaaatcc aactactgct cctgtagttt ctaaagatat
    24001 attagactct gaaacagatg caaagtatat acctctaaat ccaaataaaa ttacacttag
    24061 tattatcatg tttattacag ctatccatat aaatcctgta ctcttaggaa taaccaagta
    24121 agcagctgta cttactgtca ttaatataaa tcctacaaac attactctta tagatgaacc
    24181 aactttatct gttataacac cagcaactgg agatgctccc atttttataa cataagttct
    24241 tattattgat aaagcagaaa ctagagtcat tggcattaca taaacttctg ataagtatgg
    24301 acttaaataa gttaaacttg agaatactaa ataagttgaa aatattataa gaccaattaa
    24361 ccaagccttt ggcattgtaa ctacttttaa caatgactta atacttaatc cttcatctga
    24421 tgctccacta tcttttttct cttctactaa gaaaaataga agtataccac atattataga
    24481 tgctgcagaa tataaccata ctacatattt gaatcctact gttatttcag caaatttacc
    24541 aaagaaataa agacctataa aagaaattaa tgtacctgat acgcctgata acccttcata
    24601 aaaaccaaat attcttcctt gctctgactc acttccttgc attctaacaa ccttaacaga
    24661 ggctgagaag aaggttaata ttgtcgtaaa tccccataat acaaatatta aaagtatcat
    24721 actatatgat gggaatgtag agaaataaaa tccaagtatt cctgaagcaa ttaaagaaaa
    24781 agttattaat tttctaactg agaatctatc tgcaaaccat cctcctataa aataagatac
    24841 cattcctact aatccatata aactcaataa gttccctatt tgtgtatgac ttaatgctaa
    24901 agcttgttgc attgggtcat aaaatgatga tttcatatat ggtaaactat acatagctgt
    24961 tgttccaaat gccaatatga atattatcaa aaatctttta acattatttt tgctttccat
    25021 tgattctctc tccttaaaat tttattgttt ataatctcat tcatataatt ttattttaat
    25081 ttttctattg cttgtttaaa atcatttatt atatcatttg aagattctaa tcctacagct
    25141 attcttatta gattttcccc attcatttga actaatgtat gctcttcccc taagcttgtt
    25201 gctattgttg caagctctaa agaattcatt aatcttctac tagcttggtc tcttgtcaat
    25261 ccattgatac catccttaag tttaaatgaa actacacctc cacctaaacc attcatttga
    25321 gttttacaaa gctcatattg aggatgagat tttaatgctg gatactttac caattccaca
    25381 ttctcttctt cctctaaaaa ttctgctact gctaatccat tttcacaatg ctttttcatc
    25441 ctcatatcta acgttttcat accccttaaa atcatccatg cactcattgg tggtaaagat
    25501 gctcctgtaa aacaacataa acttggccat ctaactaaat ctattaattc ttttgaacct
    25561 gctactgctc cccctaaagt atccccatgt ccatttataa acttagtaag actatgtatt
    25621 actaaatctg cacctaaatc tattggtctt tgtacaattg gtgtcccaaa agtactatca
    25681 actatagcta agcttttatt ttcatgagca atttctgcta ttgtttttat atctactaag
    25741 tctaatgatg gatttaaagg actttctata taaactattt tagtatttgg tctcattgct
    25801 tcttttacat tttgtggatt tactgcatct acaaatgtaa cttcaactcc aaatttagat
    25861 aacaactcag acatgaataa tttagtgtgt gaaaatattc catctgaact aactatatgg
    25921 tcaccacttt ttaccaatgc taatatagta gaggttattg cccccatccc tgatgtacta
    25981 gatatggcac tttctgcatt ttcaagcata gccaacttag cttctaactc tgtaagagta
    26041 ggattacact ctcttgtata cacatatccc catgtcatac acaactcttc aaaatgctct
    26101 actgtctttg cagcaaatgt tgctgtttga catattggtg tagccaatgc acctgttgtt
    26161 gggtctgcac tatgtcccca atgtattatt tttgtctcta aattttgatt aatattgtta
    26221 ctcattttga tttcctccca ttttgactta tttatacaac gttttccctg ccaaatttca
    26281 tcaatttagc ttagtcttaa tacatttttt caagtatact tttttatttg ttataatttc
    26341 taaaaatatg acacttattt tgttttttta cagttctaat tataaactct atagtaacta
    26401 tagagtcaat atgtttttga tatttttttt acaatgttta aaat
//
LOCUS       DAKBNN010000031        25818 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000031 DAKBNN010000000
VERSION     DAKBNN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.031"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(137..649)
                     /locus_tag="OUB02_001171"
     CDS             complement(137..649)
                     /locus_tag="OUB02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884943.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            975..1232
                     /locus_tag="OUB02_001172"
     CDS             975..1232
                     /locus_tag="OUB02_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884944.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            1375..2001
                     /locus_tag="OUB02_001173"
     CDS             1375..2001
                     /locus_tag="OUB02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HCT8884945.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(2247..3026)
                     /locus_tag="OUB02_001174"
     CDS             complement(2247..3026)
                     /locus_tag="OUB02_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HCT8884946.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(3094..3555)
                     /locus_tag="OUB02_001175"
     CDS             complement(3094..3555)
                     /locus_tag="OUB02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8884947.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(3531..4208)
                     /locus_tag="OUB02_001176"
     CDS             complement(3531..4208)
                     /locus_tag="OUB02_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8884948.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSKV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            4441..4827
                     /locus_tag="OUB02_001177"
     CDS             4441..4827
                     /locus_tag="OUB02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8884949.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVAKESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            4830..6628
                     /locus_tag="OUB02_001178"
                     /pseudo
     CDS             4830..6628
                     /locus_tag="OUB02_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
     gene            complement(6981..9068)
                     /locus_tag="OUB02_001179"
     CDS             complement(6981..9068)
                     /locus_tag="OUB02_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCT8884950.1"
                     /translation="MIKVVYMRKNKKTRKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENTDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNADILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYIVIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            9429..10007
                     /locus_tag="OUB02_001180"
     CDS             9429..10007
                     /locus_tag="OUB02_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCT8884951.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            10000..10659
                     /locus_tag="OUB02_001181"
     CDS             10000..10659
                     /locus_tag="OUB02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HCT8884952.1"
                     /translation="MNKQKFHKINLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(10870..12744)
                     /locus_tag="OUB02_001182"
     CDS             complement(10870..12744)
                     /locus_tag="OUB02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8884953.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMIKDRETINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(12749..14413)
                     /locus_tag="OUB02_001183"
     CDS             complement(12749..14413)
                     /locus_tag="OUB02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCT8884954.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(14427..15248)
                     /locus_tag="OUB02_001184"
     CDS             complement(14427..15248)
                     /locus_tag="OUB02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT8884955.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(15347..16111)
                     /locus_tag="OUB02_001185"
     CDS             complement(15347..16111)
                     /locus_tag="OUB02_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8884956.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(16108..16857)
                     /locus_tag="OUB02_001186"
     CDS             complement(16108..16857)
                     /locus_tag="OUB02_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8884957.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(17361..17966)
                     /locus_tag="OUB02_001187"
     CDS             complement(17361..17966)
                     /locus_tag="OUB02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8884958.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            18145..18783
                     /locus_tag="OUB02_001188"
     CDS             18145..18783
                     /locus_tag="OUB02_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8884959.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVIGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            18921..19583
                     /locus_tag="OUB02_001189"
     CDS             18921..19583
                     /locus_tag="OUB02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8884960.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            19583..20764
                     /locus_tag="OUB02_001190"
     CDS             19583..20764
                     /locus_tag="OUB02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8884961.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTLWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGAKFIIKFY
                     T"
     gene            complement(20945..23605)
                     /locus_tag="OUB02_001191"
     CDS             complement(20945..23605)
                     /locus_tag="OUB02_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8884962.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWDSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKISASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(23602..24285)
                     /locus_tag="OUB02_001192"
     CDS             complement(23602..24285)
                     /locus_tag="OUB02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8884963.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTYGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDFLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(24629..25582)
                     /gene="blaCDD"
                     /locus_tag="OUB02_001193"
     CDS             complement(24629..25582)
                     /gene="blaCDD"
                     /locus_tag="OUB02_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HCT8884964.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKCVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         9050 a         4162 c         2934 g         9672 t
ORIGIN      
        1 taccaatttt attttggcaa ctctatttaa gtttatgttt tacagttata ttagatttaa
       61 gcttagtttc attatcacac taacttcaaa tttactctat aatccttaaa tcaaacacta
      121 atttattaat caaacactaa tctattaatt tatatttctt tagacatcta atatagtttg
      181 ctactaaata ataattattt atatcatatt tatctaatcc tttaaaagaa gttatttcat
      241 cattaagctc atcactacct tctacaaaaa cataacctgt ttcaaattcc atacctacaa
      301 taatcttaca gccactataa atttcctctt gagtatcgta caattttttt gcatttcctt
      361 catttggaat tgtaaataca ataggtttta attctttatc actggaaatc ttttgtgaaa
      421 aaggcttgtt taacttaata ttattgataa ctacatgttt aaattcctct gaagtttctt
      481 ctactattac atattttcgc tttaaaaact ctattatttc taaaacagac ttagaattta
      541 cttttaaaat acttttatat ttttcatata aagatttcca ctgttcaatt ttttcctgtg
      601 gtatttcttg gatatgtgta cttatttttt cttgagatat ttttttcaat taaactctcc
      661 tttcaatact taaatattct tatgaatctc acatgactaa agttacaagg tttctactta
      721 atagaatttt gcatacttaa atatgtctta ttaattctct acaggatatc tccgtagttc
      781 ctacaattct tacataagtt acttatttta aattgttatt agttagattc taaaaattat
      841 ttattttatc actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc
      901 ttttatttat aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa
      961 aggaggtttt ccttatgatt ttaaatagag ctgagctttt ccgaggatat tctttaaata
     1021 gatttaacga gatatgcaat atattatcag taaataaaat taaatataag tataaagtga
     1081 aaaataatgc ttactctaaa aaaccttttt ttaatgaaat aacacttggc aatttaggtc
     1141 aaaaggaaga tttctcttat gagtacttaa tttatgttca taaagacgat tatgaacagg
     1201 cagagtctct aataaattct aagttgcgat agattattta taaaacatga cattctgttg
     1261 tcatgtttta tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct
     1321 ttaaaactag ctttttatag taatttaaag cttaaaacta aggagactcg ttatatgaaa
     1381 ataaatataa aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacagc
     1441 agaataatat cctaccttcc taatataaca atagaaacag acaaaataaa aactataatg
     1501 attaatgagg ttgtcccaac taacaccaat gacgattttt atagtgtaga taaagatgct
     1561 gattatttaa aaactactat tcctttattt gacagtgcag gaataaaagt atctaacata
     1621 aatgatatcc tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa
     1681 tatacaataa ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa
     1741 ttatttaaaa acctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat
     1801 atgattacta aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt
     1861 cgtaataatg aactttatta tgataattta agagtatttc catcatatat tatgactggt
     1921 ggaaacatac tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg
     1981 ttcgagatta taaacggttg atattacaaa ataataaaaa ctaatataaa ataaaagtgc
     2041 atcaaaaact tctatttatg aggcactttt attttatatt agtgtaacta tatactatta
     2101 acataattta ttatttatat tgttcttttt aatctctatt ccatttagtc taggtaacat
     2161 tactctttac aagattactt tatatttctt ttatatctct aacgttttta tttaataaaa
     2221 caattattaa tacaagtcaa atatatctat ttatctaaaa atatatcttt tacagtaata
     2281 tctggatggt gatacttctt cctattcgtt aaaaaagatt tttttacatt aatagcctct
     2341 tttggacacc agtgaaaaca agctaggcat ctttcacact cattacccca ctttggcttt
     2401 ttatcaacta tacttatatt ctctacagga caaacccttt tacatgtccc acatccatta
     2461 catttatttt ctgcaaaaaa tttagtagtt gcaatttgca tattcttacc tactaaacga
     2521 agtacaacac tatctatatg tgactttaaa ttgttttttc cttctatatt tccaatctcc
     2581 aagttattga tacattttgc aatctcagct accctattag tctgattttt caatctttct
     2641 tcttcaacat ctggaggtgt tatcaaagca tttccaggca tattgattaa aaaaccagca
     2701 gataattttt tccccctttt tttaagtaac ttatctatag taaataaact atgtccagct
     2761 accccattac aagtggcaat ggcatatata tgagtattaa tattaaaatt taatttctct
     2821 acaaaagact ttactatatc aggaacatca gcaaaataaa caggaaaaac aattaccaat
     2881 atatcttcat ttatgtctat attattttct tttctatgta tagctaaggg tataacttct
     2941 ttacttccat ttaacgtttc ttttaaaata tttgaaacta ctagcgaatt tcctgttcca
     3001 gaaaaataat aaatcttact attcattctt atccctcttt ctatatttct acatcatcaa
     3061 tagttaaaaa gtctcttaaa ataggattta tatttacttt ccaattctat ccaaaatatc
     3121 agtaaacaaa ggcaattctt catcagagat agatttaaat gtctctgttg catatatact
     3181 tgcaatttca tttaatggtt cttctaattc tctacctaaa tctgttagga atatttgaaa
     3241 cttacgtcta tctttaacat ctactttacg gacaattaat cccttatttt tcataacatc
     3301 taagattctt gtcgtattag atttatcctt acatgatttt tcacataaat ctatctgaga
     3361 aattccatct tgctcccata gaaaccttaa taacgcataa tggtcagttg ttatatcata
     3421 tccattttgt ttaaacatct tttttaaatc atcttttact tttaacgaag tacttactaa
     3481 cttagttgta gcttcatata gtttctcaaa atctttcatt ttacctccat ttaaataatc
     3541 ccttgctcct cccatctatc aattattccc ttatattgct caatatcttt aaaagccatt
     3601 ggctttaaaa cagcatttat agcaataaaa ttatagattt gagaaagcac aggtggattt
     3661 ttcttttcct ctatgcaata aataaatttt ttagatacct tttgtatctt tgattctact
     3721 ttttcagtta taggattttg attaaatcca gtttttatag ataaactacc acaaacataa
     3781 cctcccgaat ctaagaccat ggattttata aaagaaattg cttttgaacc acgtgcattt
     3841 tcatatgtag taactgctat acatggctta cgatacaata atctttcaag catcatatgt
     3901 gctctatctg taaacacctt gaaaagtcca gttacactac ttccataagt tgggctacca
     3961 aatataacac catcactctt agtaattata tcatgaatat actctacttt atcattttga
     4021 ttgatacact tgcccatttt ataacaattt aaacaaccaa tacaatatga tggatttacc
     4081 ttacttaaat caaaataatt aatttttaca tctggtatta atctttctat atctttatac
     4141 aagtaagtta aaacttttga tgtagctcca ttttttctag ggcttccatt tataactgta
     4201 attatcattt ctatatctcc tttttagttg ttatatcaac aataagtata tcaactatat
     4261 ttttagctgt caatataaac catttattta aagctatact tacacagctt aaataaaagt
     4321 tttatttaac tacatgtgct tatttaatca actataaatg aattattttt aataattgac
     4381 aaactaaatt aatgataata tactttatgt attacaaatg taagtagaag gaggtatagt
     4441 atgaaaaagt taccacatat ttcagaagca gaatatcaag ttatgaaaat tatttggaaa
     4501 tatgctccta tcagcactac tgaagtaatt gaaaagttgg tagaaacaag tacatggagt
     4561 cctaaaacaa ttcaaacaat gttattgaga ttagttaaaa aaggagcttt aacatatgaa
     4621 aaaaatagca gagtattcgt ttatactcca cttgttaaag aagaagaata cgtagctaaa
     4681 gaaagtagtt cttttttaaa tcgtttttat aatggtactc ttaattctat ggtactaaac
     4741 tttttagaaa atgataaact atctgaagat gatattgagg aactgagaga aatcttaaat
     4801 aaaaggacga ctaaggggga taaataaata tgtatgcccc attctttata cgatttttac
     4861 tcagtacatt aaccttatca atattaattg ctgtaatttt acttgccaaa aaagtattta
     4921 aaaaacatat ttctgcaaag taccaataca atattggatt tatattgctt ataatgttaa
     4981 taattccatt tgtccctctt aaatatatta atcttggaaa tgtatttaac tatttatata
     5041 cttttaatca acttggaagc agtactaata ggacttctat gataaaaaat agtccaaatt
     5101 ctataattaa taactcaaat gcattaaacg atttttcagt atccctaaac caagttaatt
     5161 ttgatagttt aaatatttta ttactaatat catggataat tggaatttta atcatgatag
     5221 ctattactat tcattgcaac attaaaataa aaaatatgaa aaaatcaata caaattttaa
     5281 gagatgagga gatagtattt atatttgaaa agtgtaaaaa agatattggt ataaataagg
     5341 attttatact atgcaaatca cctattataa atacacctat tacatttgga tactttaaac
     5401 cacacattat attaccagat aagtctatat ctaaattatc tttaaaagat ataaaatata
     5461 tcttacttca tgaattacaa cactttaaaa ataaagatat actgataaat tatataatgt
     5521 gcttccttca aatattatat tggtttaatc ctcttgtatg gtatgcattt aaagaaatgc
     5581 gtatagaccg tgaaattgca tgtgatattt cagttttaaa aaagcttgat aaagatagtc
     5641 atatagaata tggacaaact ctaataaact ttgtggataa tatttcaaga ccatcaaatc
     5701 ttacacttac atcagatatt ggtgggtcaa aaaaacaaat aaaaaagcga ctattgaaga
     5761 taatggattt tcataatgaa tcaaatacgt taaaattcaa aagcatttta atttttacat
     5821 tgatagcttt aattgtattt ggaaatattc ccaaactatc tattatggca gatatcaacg
     5881 aaaaatatag acctacaaat gaacaaactg tgtacgagga cttaagtcag tattttaaag
     5941 gttttgatgg aagttttgtt atgtatgatt taaaatccaa gcaatatcat atttacaata
     6001 aagaaaaaag taaaacaaga atttctccaa attctactta taagatttat agtgccttat
     6061 tgggattgga aaatgggata ataacaagaa ataattcaca tatcccttgg gatggaaagc
     6121 atcattctaa tagttcttgg aatgaagacc aagacttatt ttctgctatg gaaaactccg
     6181 taaactggta ctttcagact ctagataaaa aaacagggct aaaaaacata caaacttatc
     6241 ttaggcaaat tggatatggc aattatgatg tttcaggtgg tatctcaaac ttttggctag
     6301 aatcttcatt aaaaatatca cctattgaac aggtagaatt acttgaaagt ttttatacaa
     6361 ataagtttaa atttgaagat aaaaatatag aaacagtaaa agattcattg ttaatatcaa
     6421 aaaaggatgg ggtatcactt tttggtaaaa caggcactgc taatataaat ggaaaagatg
     6481 taaatggttg gtttataggt tatgtggaaa aaatgaaaat acttatttct ttgctacaaa
     6541 tattcaaagc aaagattatt ctaatggtag ttcagcatct aagataacat tatccatatt
     6601 aaatgacaaa aaaatctata atccttaata tatttactgt acattctaaa aaattcacat
     6661 aattttcata ttaattatca attaaattca tatacaaggc ttattatata aacatagaag
     6721 atagtaaaac tagcttaaaa acataatata ataccctata caaatagtgg caactctccc
     6781 tcacatcctc aattgttgcc actatttaat tttttgtatg aagttaaata tattttcatc
     6841 atttcaacaa taaaaaagag gtctatctct attttgaaac aacctcttta attattttag
     6901 agtttactaa gaagtctaaa aacaatttat ctaaaatatt attctcaact gagtaagatt
     6961 aatttccaaa ttgagtaaat ttaatcttca aacactgatt taacaatagg aataacataa
     7021 tgacttcccc cacgactctt tacatcttca atcatagcaa ttactatata ttgtttatca
     7081 ctattttcat ctacagtaaa agcattaaac catccaagtt ctgtccctgt aacatcatct
     7141 tttgatgctt taatttctgc tgtaccagtt ttcccagcaa tgctaattcc tttaatcata
     7201 gcactatgac ctgtaccatc agggctttca acaacttgaa gtaagtcatc acgaataatt
     7261 tttacagcct ctttagaaaa cacacctttc ttccaaaatt cagccttttt aaattcaaga
     7321 tggggtttta tcatgtctcc atcatttaca aaagctgaat atattgatgc cgtatgtact
     7381 ggatttacaa gaagcttacc ttgtccatag ccagtatctg ctaattgtat ttcagtctca
     7441 aatttattgt cagtgccaaa ttttgaagaa gacaatccaa attcaaatgg catactttca
     7501 tcaaatccaa gcttcagcaa ctcttttgct aatacatctt cacctatttt taaggctgcc
     7561 ttagcaaaat aaatattgtc agaataaatc aaagcatttt tcatatttgc agtatttcca
     7621 taatctttta acgtggttac cttataactt ccccaactag aatctttttg ccaacttaga
     7681 ctgctgtgtc caaagttttc atttggatta atctttcctg ttgttatacc aataccagca
     7741 gtaactggtt taaaagaaga cccaggacat aatcgtgctt taaatctatt gtacattggt
     7801 ttattttcat ttttattgag ttcattccat ctatcttctg acattcccaa tataaactca
     7861 tttggattgt acgacggact acttacaagt gataaaacct ctcctgtttt aggattcata
     7921 actactgcac aacctttatc atttttcatt tggtcatata gtttactctg tagttttgaa
     7981 tctatagtta gctttacatc ttctccatct acctttggct cagttactaa agtttctttc
     8041 ttgtcaccat atctatcagc tattataatc tcatatccat caataccacg tattctatct
     8101 tccagtaaat tttctaaacc aatcttacca attatactat tatcgttgta cctcttaccc
     8161 tctttttcct ttagtatatc tgcatttaca ttttgaacat aaccagttaa atgaccggtt
     8221 ttttcaccaa atggatatac tctaacctct gtattagata ttttaactcc aggaatacta
     8281 agaagtgact cttgtagttc cttatcttct tcttttacag taccatctgt attttctttt
     8341 attttttcaa tagtttttat aggtaccatt gaattttctt ttacccaaga tgcatttaat
     8401 ttcttttcaa tttgttcaac tgatacattc aataaagtag ataatttttt taagtcttct
     8461 atattatcac tcatttttcc aggaactaat cctatcatag atgcaagacc ttgaccagca
     8521 atcattacac cgttcctatc caggatacgt cctctcttac ctttcagctt tgataccctt
     8581 acagtattat cttcttcaag ttctggaaat atgacgtttg actgccattt tattttataa
     8641 tttttctgtc tatctctagt taatagtact tcatttgaaa atgaaatttc acctgctaaa
     8701 gtatccatct tagaatcata gtttattact ttatcattat ttttattttt tttaatatca
     8761 ttaatcttaa tacttatatc gtgtgcatca attccttcat agattttttt atttcttaaa
     8821 acaaaatctt cttttttatt ttcagacttg ctcttttcat ctaataaatc atacattttc
     8881 tcatattgtt ttttatttgc aaaagacata tactgcttaa acacttcatc tggtttgttt
     8941 ttattgagtc catctagtat gaattttgct aaaaaaacaa taataactat aagcagacac
     9001 attagaaaac caaaaattat catttttctt gtttttttat tttttctcat ataaaccacc
     9061 tttatcattt aatatattac atacgtaagc tttaaggtta aaatactctt atagacataa
     9121 ctaatctatg tttttattat gtttttaagt atctcataaa aatatgtaag cagtataatt
     9181 gatagtaact tatatattac acatgtaagt tttaaaagtc aataatatta tcattattta
     9241 attatttttt acatcaactt aaaaataaat cagttccatt atattctttt gtattcttca
     9301 actattttat aaattaacct tattctattg taattatttt atagtatata actttaactt
     9361 gacaaaacga cacaagtgtc tcataattaa aagtgacact tatgtcgtta aaaaggaggt
     9421 taaaacatat gccaacaaaa ggagaacaaa ctaaacaatt tatcattgat acatcaataa
     9481 tgctattttc tgaaaaagga tatagtaatg tgacaatgaa agatatatgt gaagcatgta
     9541 atttaagccg tggtggccta tatagacact ttaattcacc aaaagaaatt tttatcagta
     9601 tattaaacac tcataaatac tactctcaaa atttgttgga taaaggtatc aaggaaaata
     9661 tatcagctaa gcatatgcta gaatattttt tccaacaaca aaaggctgac ataaatggca
     9721 aatattgtgg atttgatttt gcaatacacg aatttgcttt tgtagaaaaa gaccaacgtg
     9781 attatatgaa taaaagatac agttcagcta tcacaatgct ttcaaattta atcgattatg
     9841 gacaatctaa aagtgaattt aaaaaatgtg attctaaatc agttgcaata cacataatgt
     9901 tcttatttga tagcatgaaa acatctgctt ccattttgaa acttacaaat aaagacattg
     9961 ataatcaaat aaatattata aaggacatgg tgattttata tgaataaaca aaaattccat
    10021 aaaattaatt tacaaaactg ggaacgatat acacattttt actacttttc aaaaatgaca
    10081 aatgtagggt ttaatttgac tatagaaata gatattacta atttgcttaa ggaaacgaaa
    10141 aaaagaaata ttaaatttta tccagcgtat ttatatacaa taactacttg cttaaatcga
    10201 tatagagaac taaaaatcgc atatgatgaa gatgaactcg gatattggaa cacgttaacg
    10261 ccactttatg cgatttttca tgatgataat aagactttct catcaatatg gacagaatat
    10321 gatgaaaatt tctcaatatt ctatgaaaat tatttagctg atgttaaaga atatggaaaa
    10381 aatcatggtt ttctagcaaa accaaatccg ccaagaaact gctatactgt ttcctgcctt
    10441 ccatggatta gctttaagag ttttactgta cataatcatg gtcttgaaaa ttattttttc
    10501 cctacaattg aagcaggtaa atactatcaa aaagatgaaa aaatacttct tcctctttct
    10561 ataacagtgc atcacgcaac tactgatgga taccatgtaa gttgttttat tgattacata
    10621 caaaaaatgt gctttaatac taagatatgg ctacactaaa aatttataca atataaaaag
    10681 aactgtcgca tgagcaaatt aaatttgcta tgagaaagct cttttttact tacaatagtt
    10741 aatatgagat gaattatttc ttatactttg aacttaccaa tagttaatat gaaatgaatt
    10801 atttctcata ctttaaaaag attgaattac tcttgataat cttcttttgt agtcattaca
    10861 tatcacctac tactcaacac atattctttg acctattttt acatccttgt ttatatccaa
    10921 aatatttacc ttcttaccat ctggaaacaa taccattgtt aaagtagact ttcctaattc
    10981 gtgtattctc tttaaatcaa cattaacaag aacgtctccc tgtttaacca tatctccttg
    11041 tttaacagca acatcaaagc catctccatt taatgcaact gtatcaattc caacatgaat
    11101 taatacttct ccattagttc ctttaatccc aaatgcatgt ccacttgaaa atactgtttc
    11161 taatttacct gaaataggag aaacaatttt tccatctata atatcaatag caaaaccatc
    11221 acctaacatt tttttagaaa atgtttcatc tggtacattt tctaatgcaa caattttccc
    11281 cttagctgga gacacaaagt tttcattatt ttcattaaac actaattctt cgtcttcatc
    11341 ttcatttata gcttctttag gcactccaaa taaacatgtt acaataaagc agaatgcaac
    11401 cacaaatatt gatactaata agtatgtgat taaattatat ccatcataga tatacattaa
    11461 ataagaaggt aaaactgcta atccatatga attagattga atatgtaaaa ttgataatat
    11521 tgctgctcct aaacctgaag atgcacacat aataatcaaa ggctttaaat tatatcgaag
    11581 ctgaatccca aataaaacag gctcacttat accaaaaagt tgagatagca ttgctccaac
    11641 tgcagtttgt ttaacttgat tatttttaga ttttttcata aatgctagac atgttccaat
    11701 attcgcaaat ccatacattg cacaaagagt aataagggca ttaaatcctg tatttgctaa
    11761 tagtgatgtt tcaatcatta cataagtatg atgtaagcca gtaataacag ctaatggata
    11821 tgtaaatcca ataataaatc ctccaattcc taaaggcaaa tcaatcaata agctgatgat
    11881 atcaaccatt tttaattcta ttgtatgcat tatagggcct acacctaata aaataactaa
    11941 cattgttatt agcataacaa agaatggtgt gaaaataagg tctaatacat taggcatatg
    12001 tttacggaac catttttcaa gattagctcc aataaatgct gtaacaatag ctgttaaaac
    12061 acttccttga caacctacaa taggaataaa tccaaatgcc ataattgctt caatttcatt
    12121 tgatgggtca gcaacagaat atgcattagg aagtattggg gaaactaaca taagtccaat
    12181 aacaagtcca ataacaggtg ttcctccaaa tactttaaat gctgaccata caataattgc
    12241 tggtaaaaaa gcaaatgctg tttctgtaag aaccgaaaca agtgtctgaa tatacaatgg
    12301 aatatttgca gaagacattc caaataatcc aagtacatta tcattaaaca aacaaccctt
    12361 taatcccaag aataatcctg ttgctgcaat aactggaata ataggaacaa atatatctgc
    12421 tagagttctc ataactttct taaatccagt ttcattattt ttaactaatt catcttgttc
    12481 tttttttgat aaagttttta atcccatttt ttcaacttct gcataaactt tattaactat
    12541 tcctgttcct aaaataattt gatactgtcc agaagtaaag aaaacacctt taacctcatt
    12601 gaccttttcc actttcttat cattaatagt ttccctatct ttaatcatca cccttagtct
    12661 tgttgcacaa tgtgtaatag aaataatatt atcttcacca ataattttaa taagctcatt
    12721 tgctatttta ttatatttat tcatttttct acctcgcttt caataatact gcatgaaatg
    12781 gtttcaaaca cactgtttta tttaatagat ttatatcatc taaattttgg aatacaatat
    12841 catatttttc ttctactctt atttctacag gtttatcaga aaaattataa taacaatcaa
    12901 taactaccaa atctaattgc cttctatacg caataacatc ttcactttct atatcaatag
    12961 gttctattga accataaatc aaacaatcag aatactcact atcttgtctc aactttatca
    13021 tctttttata aaattctaaa atactctctg aattgttaat ctgactctgt gcattaatat
    13081 ctttataatt ttcaataacc tttaaccatg gtgttgtatc tgaaaaacct gcattttctt
    13141 tatctgccca tggaaatggc gttcttgcat tatctcggct tcttaaattc acaaattcta
    13201 acgcttcttc ttctgagtaa ccttcatcta ttgaacgata gtattggtca atgctagata
    13261 aatcattaaa atcatttatg gtatctcttt taaaatttgt cattcctaat tcctgcccct
    13321 gataaataaa tggggttcct cttaaaaaga aatacattgc tcccaacatt tttactgcat
    13381 catcattcct ttcgtattgc aaaagatact ttgttgtagc tcttggttgg tcatgatttt
    13441 caataaaatt agctgcccat ccatgttcct gaacatacat ttgactatct aaaatgcatt
    13501 ttcttaaatc tggaattgtc catgaaattc ttttaaacca ctcactacca gaagcaacat
    13561 ctaagtcagc atgtttaaag tcaaagacca ttgaaaagaa accatcttct ccaataaaat
    13621 cacctaactg gtcatagcta atacctgctg cttcagcaac agtcatacaa tcatattttt
    13681 taaacgtatt ttcttttaat tcataaagca ttttttctat tccaggctga tttctaactg
    13741 tttttgtaca tttaacaaga ccatcaacac catcactttc taaactttga aaacttaaat
    13801 tctttttgat gaatgtaata gcatcaactc taaatcctgc cactcccatt tgagaccagg
    13861 tattaatcat ttggtaaagt tcacttctta atattggatt ttcccaatta aggtcaggtt
    13921 gttttttacc aaaagaatga aaataatatt cgtctctatt ctctaccttt tcccaaacac
    13981 ttccaccaaa aacagacctc cagttatttg gtaaatcgtt tcctttttta aaaatataat
    14041 aatcatgata aggactttct ggatttttta atgcttcttg aaaccaagga tgttcatcac
    14101 ttgtatggtt aagaactaaa tctaatataa ttttgattcc tctatcttta ctttctttaa
    14161 ttaactcttt taaatcttcc atacttccaa attcacttgc aatatccaaa taatcactaa
    14221 tatcataccc attatcgtcc ataggtgatt taaaaattgg acataaccac aatgatgtaa
    14281 tacctaaatc ttttaaataa tctaaacgag aaataattcc ctgtaaatct ccaattccat
    14341 cattattact atcttgaaag cttcttggat aaatttgata aacgatttct ttttgccacc
    14401 atttcttttc cataaattac tcctttttat atgatatttc ttctttttaa ttctttataa
    14461 attccattat cgaatatgtc ctcacatata gatgtcgcta tatctttaag ttgttgatga
    14521 ctatttttca ttgcaattgt aacgtctgat gcttgaaaca tgacaatgtc attttgacca
    14581 tcaccaaaac atatggtttc tttttgagtt actcctaatc tttcttgtaa tctcttaatt
    14641 gctttgccct tatgaaaatc tttttgaata atttcataat actgactaga aatatctctc
    14701 tgtgctaatt ccattttatc ttgtagtata tctttcactt cgtcaaatac cttttcattt
    14761 gaccataaac aaattttatg tatatcttgt gacttatact cttcaatgtt attttcatat
    14821 gttatttttt cttggatatg ttgtttattt atacaagaat tagttccttt caactgattc
    14881 attgtttcaa atatttcttt ggctttttga ttcatgaaaa ctttttcctg actctcaatc
    14941 gaaaaagcaa cttccctctt ttttaataaa caaacaactt cctttatcaa tctttgatta
    15001 aaagattgat tataaagtaa ttcaccatga tattgaatgt aattacctcc tcctgcaata
    15061 tatccatcaa ctcctaatga taaaacatca tcttgtattg tacccatact tcttccagta
    15121 caaataacaa cactacaatg attcttttga cataaacgta tagcatgctt tgcagattct
    15181 gggattccat atacctcatc tctaagcgta ccatctatat caagtattaa taatttgtac
    15241 aatttcatct tcctttctta ctatcaaaat cataatagca ttcaaaaaaa aagatatcaa
    15301 tattttgata tcttttaaaa aatatgttca tttttagcac aaattttcat tttcggggaa
    15361 tatgttcact ttctaaatac atcttgtatt taatataaag cagttcaatt aaaataaaat
    15421 aaggtgttgt tacatcgtag tccagttgtt tatctacaga aatagatata gattgaatat
    15481 acaggttttc atcacataat cttgctaaag gattacttat catttttgta atagaaacca
    15541 tataaattcc tttcattctc atttctttag caaaatcaat aattgtagat gcatctccat
    15601 tcaatgaaat aaagaaaatc atatcatctg tttttataaa atgattaagt gcctgaatca
    15661 tatcataacc ataaacatca tatataattt tccctgttgg taagaaaatc cttttcatct
    15721 ctttagcaac tctttcctga gaataacctt ctccaaagac gattatacga tttgcctttt
    15781 tcattctttc aaacataatc gaacaatcct tcttctctat atcatctatc accttattat
    15841 aacaatccat taattggtgt ttactttgat ttttatattc tacttctgtt tcatttaagc
    15901 gtataataac tcttaattca ctatatccag gtaattgtag tttttgagca aaacgagaaa
    15961 gtgcacttgt agatatatgg tatttgtctg caaattcatc tatagaaagt ttaatacaat
    16021 cttctttatg attaagaata cactcacaga tatacttatc atttgctgta aatgaattat
    16081 aatatttatt gaaaagtcct tcaattttca ttcttttctc cttctatact ctatgtaatt
    16141 catatgaagc aaatctaata gagcataaaa tgctcctacg acttcataag atatagcttg
    16201 taatgtatca agtttttgag tttcaacata cagattatac tcacaaagtg ttgcaattgt
    16261 attattatca agcctagtta aagaaatcgt atgtatatga ttgttgataa attttgcaat
    16321 ttcaattcct gcttttgttt ctccagataa agaaataata ataaataaat cacttttttg
    16381 aaatgattct ctcataaatt caatttcacc tatatcaaat aagtcaataa cacatttacc
    16441 tactgaaaga aatattctct ttatttcttg agctaaactt ttctgttcat tacctgtccc
    16501 ataaatataa attgtttctg catcatatat tattttacaa atatcttgaa atgatatttt
    16561 ttgtaattca ttcattaata gtcgataatt ctcgcaaaca tcatgaaaat tattatcatt
    16621 attaacaaat tctagttgat tatttttaag taaatacttt aaatcagaga atgaatctaa
    16681 tccaattttt ctacaaagcc ttaataaagt agctctagaa acattacatt catttgcaat
    16741 tttttcacac gaaaaattac ggtattctct ttgacaaatc aatatttgtt taataataat
    16801 tttatcattt tcatttaaat tatcataatt ttggttaact aacgattcta atctcatttt
    16861 ttccactccc taaatattat tttaatatac attagttgct ttttctaggg aagagtttta
    16921 aataataagg ataaaataat catttgattt tatttttatt aaaataaaaa taacctgcta
    16981 aaagcaggca ccaaaaagta cttatccttt cgaacatgta ctttagatat tgctttcttc
    17041 ggaagcttca tctcttcata ctctaaaata gagttttttc taactcaaaa gtattttcca
    17101 tggtagaaat tactttaatt catctgccat ttatttttta tcttatttaa taattactac
    17161 tacttgaata tttacttctt taagtaaatt tcatagttaa caaagctaaa actattatta
    17221 tagatgttaa aatacaaaat tgtaagtatt gttataaata tgcttacagc tacaaatata
    17281 catgatatat taaaaagaag ctgtaaatta ttatcataca tacaagcaac tacgcctcta
    17341 ttaatccatg ttaattttta ttatttaata caaggagttc catgagacat acatatccat
    17401 tcatatttta aattatccaa ttctcttata gatttcatca tctttttata atcccaattc
    17461 catggattag gataaggaac tatatttcca ttcttatttt tgactaaatc acctgcaaat
    17521 aatacatctt caaaaatcat gcagacatgc cctggagtat gaccaggagt atggataatt
    17581 tttatattcc caatctgcat attctctcca taaggttcaa catccttaat gagccttctg
    17641 gaaattgcct ttccaataaa cttcttaaac ccaggtctat ccttctctcc tataataaaa
    17701 ggtatatcct ctacatgagc ccatactttt gctcctgttt tttctttcaa taatttaata
    17761 ttagatatat ggtcaacatc atgatgagtt aataaaatat gcttaatatc tgtaagttta
    17821 ataccaagtg tttttaattc tttaattata tttttcccca taaaaggaag acctgtgtct
    17881 ataagaataa tttctttatc aaacacaata aaacaatttg agccacgagt actatctaac
    17941 attaatacat tttcttttat cttcataaat aaattcccct tatttggtat tttacctata
    18001 taattaatta ataaagttat aaatagttat aatacttgat attaatttta ttttttatta
    18061 aagtatacta taggtataca agtattatat ttacataagt ataccatcag tatacttttt
    18121 tatcaataga aaggaataat ttatatgtca gaattatcaa aaagagataa agaaaaaata
    18181 caaagagaaa atgagattat tgataaggca gaaaaattat tttgtcttaa tggatttgat
    18241 aatacaacta tgaatgaatt agcaaaagaa gttgaatata ctaaacgcac catatacaag
    18301 tacttttcat gtaaagaaga tttattcttt gctgttgtct taagaggata taaaagactt
    18361 tgggataatg ttaaaataga aagtgcaaaa ggcaaaacag ggtttgaaaa aataaaactt
    18421 tcatactttg cttttcataa attttactct gtagaacctt ctctattatg ccttatggga
    18481 atgattggta ttgtaaactc tgaaaaatcg gatactgata tgctgtttaa agaaaagttt
    18541 ttttctttta ataaatttat gtttgatgaa atacaaggaa tgtttgaaat tggaaaaaat
    18601 gatagaagta ttaggcatga tattgaaata cctactctta tgtattcatc aatatttaca
    18661 cttacaggat tttttaattt actatctgta ataggaaaat catacctgaa caactttaat
    18721 atagatgagg aaaaatttat tgaaacaact cttgctcttt taatagattc aattaaagca
    18781 taaacctaag tttataaatt aaagtttata tacttaaatt taaatgataa ttttttctat
    18841 ctctaatata aaaaatatta aacataaaac taataactta gttaatggaa taactaaatt
    18901 actgaaagga ttaaaagtaa atggctaaaa tacttctttt agaagatgat gatacaatta
    18961 catttggaat aaaaacttcc cttgaaaaga aaaatcacac tgtatgttgt tataatacga
    19021 ttgctgaagc taaaaaaaat tttgataaaa ctattgattt aattctgctt gatttaaatc
    19081 tacctgatgg tattggatac gagttttgcc aatatgttaa aagtatagaa gatactccag
    19141 tgatattttt gacagtccgt gatgatgaaa aagacattat acgtggatta gatatgggtg
    19201 cagatgacta tgtggttaaa ccgtttttgc tctcagtact tcaatccaga attaatgcag
    19261 ttcttagaag aactcattca aatactaaca atactattac atgtgaaaat ataagcttga
    19321 ataaggttga agtcaaagca tatttagata atgttgaagt acctttgaca gcaggagaat
    19381 ataaacttct tctaagtctt atggaaaata aaaatagagc tatgactcgt acaaagttac
    19441 tagaaatgct ctgggatgta gatggtaatt tcgtaaatga taacactctt actgttacaa
    19501 taaaacgtct tagagaaaaa ttaaaaaatc caccttgtat aaaaacatta agaggcatag
    19561 gatatagaat ggaggacaac tagtgaaaac tccaagaaat atcaacagta cagttgtttt
    19621 tataatattc tgcttaattg taactttttt attgagtgta attctaacaa ctataacttc
    19681 ttttaatgaa tacaatgtac tgtctaaggc tcttggtgca tcatatgagt acaatcctaa
    19741 gtctacagaa agtatactaa aatcactgaa aaattctagt gataaggatt ttaaaacagg
    19801 aaatgaaatt ttatcaaaac atggatattc taaaagtaca ctttggagta aaaacttttt
    19861 ttattttcta gtgatatcgt ttggtataat ttcaacaatg gctacagttg tatatataat
    19921 caaacataga acaaataagc aaaaactgag tagaataaac aatcttactc aatacttaga
    19981 agaagtgaat ttaggaaaag agtctgtact tttacgatgt gaagatgaat tttctcacct
    20041 tgaagatgag atatataaaa cagttgggga acttcgttgt tcaaaagaat ttgctttaaa
    20101 agaaagacaa actctttcag ataatcttgc agatattgct catcaactta agactccaat
    20161 tacttctatg tcacttatgg cacaattact ttcagaaaat tgtccagatg aagaaataga
    20221 ttatattaac aggcttacaa atcaaatctc aagacttgag agactagttt catctttact
    20281 tacattatca aaattggatg caagtacttt aatatttgaa agcaaactta tagatgtaca
    20341 ttctcttctt acttatgcaa ttgagcctat agaaggttct ttaagagaaa aacatcagac
    20401 atttactatc gaggcttctc cttcggtaga atttgaaatc gatattaatt ggactttaga
    20461 agctctttta aatattctta aaaattgtag tgagcatata gaagatggtg gatatatagt
    20521 tgcttattat agtcaaaatc ctctatatgt tgagattatc atagaagata atggtaaggg
    20581 gtttgctcag gaagaacttc catatatttt taatagattt tatcgtggta aaaatgctag
    20641 taaggacagt attggtatag ggcttgctct atcaaagtct attatagaac gtcaaaatgg
    20701 tactctacat gcagaaaatt gccataatgg tggtgcaaaa ttcataatta aattttacac
    20761 ataaaatgct ggattatagg taaaatacct ataatccagc tatatctata ttctatgtta
    20821 tctattgttt tagtaatata cttctattta catttaaggt tttattaaac aaacaaatta
    20881 actatcccta tacttgtaag tagtcttaaa tcgaaacctt taaataagtt caaatagatt
    20941 ttgtttatat tgtttcattt tttatagatt ctactatatt ttcattcttc aactgcttgt
    21001 atcctgtcat atatgatgct attacaataa aaagtatcaa tatagtaaaa cctattattg
    21061 tagatatagg tgcaaatggt aaatactcta taaaatatat ttcatttata cgtagaaaag
    21121 catacactat tcctatttgc acaggaatac taagtagtaa tggtgtaatt cctaagaata
    21181 aaccttccaa aataagcatc ttttttatgc cttctggaga taatccaaca gaacgcaaca
    21241 tagcaaactc ttgtcgtcta cttctaatat ttcctgacac tgtagcaagt acatttgata
    21301 atccaatcat agccaataaa cctgtaataa agcctactat taaattcatg gtctttattc
    21361 cactagcctt ttgtttttca ctttctaaaa catcctcaat actataatct cctgaaccat
    21421 agtctttatt ataaatggac tctaattttt ctcttactct aggaatgtcc tcacgattat
    21481 ttactttaaa tactcctatc agcctatttg aacgtaacct acttacttca tcataatttt
    21541 tagatatttc ctgcaccata gacattggca ctatttgcat taatgtatat tttgaactag
    21601 tatctactat tttaggaagt gtgcttgtaa tagctcccac tttcatgttg taagtataat
    21661 cccctttatc ttcgtcatat actttttcat ttaatttaat gttatctcct attgaaagat
    21721 ttaaataatc tatatatact ttatttctac gtgtactacg attaatatct tcctccttat
    21781 tatatacaat tgaggttgga ttttttgtat cataaaataa ctttgggtct acagaaattt
    21841 gcctacaata ttctgtaaaa ctcttatcat caagcccaat tatatttgaa attatacgaa
    21901 acttgccatc acgttctata ggagagtatt ttttagatga aactatactt ttaaatcctc
    21961 catttttcgc aagttctttt gatgcatctt tttcagtcag ccaagtggaa gcagatattt
    22021 tttgataata tagtgaactt tttacacctt ctgtattatc taatttttca atgaaagcag
    22081 actctacagg ttcactattt tccaaataaa ctgctatatc tctttggtcc ttataattat
    22141 cacttccata aacagctttt gctccttctt gatttgcaat tatatgcaaa aaacatgtaa
    22201 gtaataaaaa agataatgtt aaagatattg ttgcagtacg ataagatttt ttacgagcac
    22261 gtagtgcgtt tgatgcaagt tctccttcta ttccaaatat ctttcctaat gaatatttat
    22321 gcgacttttt gattttaaca ctatctcctt gtcgtattgc ttctatagga gatatctttg
    22381 atacttttcg tgctggtata agtgcagaaa tccagactgt tactattgta agtaaaactg
    22441 ctggcagaaa agcaggaata ccataagtaa ataccacttc tggaacatca gtatgataat
    22501 taactgaatt aacataaact ataagcctat tacacaataa ccatcctaaa aataaaccta
    22561 ttggtaatgg tataattgtc agtaaaaatc cttcaaacac tacagacctt ttaatttgtt
    22621 ttggtgatgc accaacacta gataatatcc ctaactgagt aagtctagca tttgcagaca
    22681 gtgaaaacgc gttgtaaatc accattacaa acacggctac tgtaaaaatt gctattgtaa
    22741 gatacataat aggagttgaa aattttgata tactactcat agatgctttc tgttctggtg
    22801 aaaagactaa catctttaaa agataattac tattgtattt aagattgtat tttccatatt
    22861 cattagtctt ccaccccaat gatttagcaa gttttggaag ttccttatac gtgtctctta
    22921 tatttttaaa tcgcaagtac acagttatac tatcattagg ttttatactt gttttatcaa
    22981 gatatccaag agctgtatat gcaggtatta ctgatgaagt agaaacatct acctttccta
    23041 ctattgtcaa tgtagtctct gaaatttttt taaaactttc ccctttcgat tttggggcta
    23101 tgggttcaag aggctttcca tttaggattc tatctcctat tgggagagta attttatcac
    23161 caatttttac acttggatta ttctcaaaat attgttttga taatgcaatt tcattagcat
    23221 tatttggtcg acgaccctct attatagtat ccttttctgg catagaatcc caatactctg
    23281 atgtagcatt tctataaatt atataatctc ttttctcatc attaattttg cctacatacc
    23341 attgtccttt taccattaca tcatcaactg aagaaaaatt ttcaattaat ggtaagtcac
    23401 ttccataagt atagtcaaac aattcaccat gccagttacc ttctttctca attgaaagtc
    23461 ttataaaatc tgtccacatg gtatacaaca gtccacacaa agaagacatc atggttgtag
    23521 taagtaatat agctaacatt atagctatac tactacgttt attgcgtcgt acaaagtcta
    23581 aggtgtattc ttgaagaata ttcatttctt caccaccttt tcagagacaa tcactccatc
    23641 ttccatagtt acaatacgat ttgcctctag tgctattttc tcatcatgag taataagaag
    23701 tattgtctga ttaaatcgtt tatttgataa gtttaagaaa tctacaattt cttctgaatt
    23761 ttttctatcg agattaccag ttggctcatc tgctaataaa atagctggtc tataaattaa
    23821 acttctagct atagataccc tttgttgttg tccacctgaa agttgacttg gaagatggtt
    23881 taatctatca gataatccca atatagatac aatctcagag aatctatcct catccacctt
    23941 tcttttatca agtaacatgg gtaataatat attttttcta acatcaagag taggtataag
    24001 attataaaat tgataaataa gacctacttt tctacgtcta aaaactgcaa gagcttcttc
    24061 ctttaaactt gatatttctg tatcttcaac ataaatcttt ccatatgtag gtctatctac
    24121 tcccccaagc agatgtagca atgtagattt gccagaacca gatgctccta caatggatac
    24181 aaaatcacct ttttgaactg ataaatttac attattaagg gctgtcactc tggtttgatt
    24241 tgaaccatat atttttgtga gattttcaca ttttaatatt tccatataca atcatctcct
    24301 ttatggttaa attttagcaa cctaaaatga cattatagtg acatgaatac aaatataaat
    24361 tactattaaa aatattgttt agtttttgca cattatttaa aattataata taaaacaaaa
    24421 actatgcaat atattttatt tatactttta atatattaca tagtctttaa aaggtattaa
    24481 ctttaaatct ataggtattc aatttaataa tcaatagcaa gtattttatg tactaaagga
    24541 gttttgctct atatagactc aatttagtaa aattatttaa taaatattac agactatagg
    24601 taaataatat ttacaaataa aattaagttt attcccttac actataatat ttcttaatta
    24661 tattaattgc aatttccttt acctttggcc ctgtaatttc tttattggaa tcatctgatt
    24721 taatagcaaa ataataggtg tcaccttcac gctctagcat accaacaaac catgcatcca
    24781 cacatttatt tttttcatca aatcctgttc cagtttttcc atatacattt atgtttttat
    24841 catttacatc tattttcata atatctctta aaatattaat atgctctttt ttaaagtttg
    24901 tatctccctc aaatattttt gccattgttt gcacttgttc ttttggagat atttgtaatg
    24961 aagattctaa ccaaaatcca tttaaatgtc cctttccatt ttttaaatct ccttcccatt
    25021 cgctaatatc gcaattccca tactttaaat catctaaaat gttttggaca gatttagcat
    25081 caactttatc tatcaatttt ttgtagtacc aaacacaaga ctccttaaat gcctcttcta
    25141 aactaagatt tttattccaa tttttatttg gatactctgt accatcatat cccataactg
    25201 attcttttga gtttattacc cctttttcta aaccaattaa agttgaaact attttaaaag
    25261 tagaacatgg tgaccttctt gtttcaatta attctttgtt atatatatta tattctttat
    25321 ttttagtatt ataaaaaata gctcctccac taataccttc aaaacaatca ctatagtcaa
    25381 ccatattaat tttttctttt ttgtttttat tattgttttc aattttggat ttatttaatt
    25441 cagacttatt taattcagat tttatataat tcttatcagt tttttgattt atatcgttat
    25501 gttttttccc tacataatac attaccataa ctacaccaac caataagata gctatccaaa
    25561 taaaattctt ttttcttttc ataatttata tcatctccca tatttaaatt tataacctac
    25621 tattaaaata gtagacatta tttgtaatat actccaatct aggttctact tataagaaac
    25681 aagactaaat gaatagctgt atctcttata ttttcttcac ctagtacaat tatgttgatt
    25741 aaacctattg cgaagataat taagtaacta ttaataacat cctgtcctcc ttttaatttg
    25801 tgatataata attgattc
//
LOCUS       DAKBNN010000032        25815 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000032 DAKBNN010000000
VERSION     DAKBNN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.032"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            14..2662
                     /gene="polA"
                     /locus_tag="OUB02_001194"
     CDS             14..2662
                     /gene="polA"
                     /locus_tag="OUB02_001194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCT8884965.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2671..3273
                     /locus_tag="OUB02_001195"
     CDS             2671..3273
                     /locus_tag="OUB02_001195"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCT8884966.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKIFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3266..3820
                     /locus_tag="OUB02_001196"
     CDS             3266..3820
                     /locus_tag="OUB02_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCT8884967.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            3820..5535
                     /locus_tag="OUB02_001197"
     CDS             3820..5535
                     /locus_tag="OUB02_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8884968.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5608..5820
                     /locus_tag="OUB02_001198"
     CDS             5608..5820
                     /locus_tag="OUB02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCT8884969.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6021..6620
                     /locus_tag="OUB02_001199"
     CDS             6021..6620
                     /locus_tag="OUB02_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884970.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            6848..7219
                     /locus_tag="OUB02_001200"
     CDS             6848..7219
                     /locus_tag="OUB02_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT8884971.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7351..8625
                     /locus_tag="OUB02_001201"
     CDS             7351..8625
                     /locus_tag="OUB02_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCT8884972.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            8736..9680
                     /locus_tag="OUB02_001202"
     CDS             8736..9680
                     /locus_tag="OUB02_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8884973.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            9896..11233
                     /gene="rsxC"
                     /locus_tag="OUB02_001203"
     CDS             9896..11233
                     /gene="rsxC"
                     /locus_tag="OUB02_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCT8884974.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11256..12233
                     /locus_tag="OUB02_001204"
     CDS             11256..12233
                     /locus_tag="OUB02_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HCT8884975.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12233..12802
                     /locus_tag="OUB02_001205"
     CDS             12233..12802
                     /locus_tag="OUB02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HCT8884976.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            12815..13408
                     /locus_tag="OUB02_001206"
     CDS             12815..13408
                     /locus_tag="OUB02_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HCT8884977.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13423..13998
                     /gene="rsxA"
                     /locus_tag="OUB02_001207"
     CDS             13423..13998
                     /gene="rsxA"
                     /locus_tag="OUB02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCT8884978.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14014..14985
                     /locus_tag="OUB02_001208"
     CDS             14014..14985
                     /locus_tag="OUB02_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HCT8884979.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15205..15789
                     /gene="maf"
                     /locus_tag="OUB02_001209"
     CDS             15205..15789
                     /gene="maf"
                     /locus_tag="OUB02_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCT8884980.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            15834..16493
                     /gene="radC"
                     /locus_tag="OUB02_001210"
     CDS             15834..16493
                     /gene="radC"
                     /locus_tag="OUB02_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCT8884981.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16614..17624
                     /locus_tag="OUB02_001211"
     CDS             16614..17624
                     /locus_tag="OUB02_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HCT8884982.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            17634..18527
                     /gene="mreC"
                     /locus_tag="OUB02_001212"
     CDS             17634..18527
                     /gene="mreC"
                     /locus_tag="OUB02_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCT8884983.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18542..19030
                     /gene="mreD"
                     /locus_tag="OUB02_001213"
     CDS             18542..19030
                     /gene="mreD"
                     /locus_tag="OUB02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCT8884984.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19042..22020
                     /locus_tag="OUB02_001214"
     CDS             19042..22020
                     /locus_tag="OUB02_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCT8884985.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22118..22801
                     /locus_tag="OUB02_001215"
     CDS             22118..22801
                     /locus_tag="OUB02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCT8884986.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            22825..23622
                     /gene="minD"
                     /locus_tag="OUB02_001216"
     CDS             22825..23622
                     /gene="minD"
                     /locus_tag="OUB02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCT8884987.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23640..23924
                     /gene="minE"
                     /locus_tag="OUB02_001217"
     CDS             23640..23924
                     /gene="minE"
                     /locus_tag="OUB02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCT8884988.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24009..25139
                     /gene="rodA"
                     /locus_tag="OUB02_001218"
     CDS             24009..25139
                     /gene="rodA"
                     /locus_tag="OUB02_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCT8884989.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25180..25593
                     /locus_tag="OUB02_001219"
     CDS             25180..25593
                     /locus_tag="OUB02_001219"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCT8884990.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT        10230 a         2821 c         5055 g         7709 t
ORIGIN      
        1 ggagtgatat aatttggaaa aaaaattaat tataatagat ggaaattcaa taataaatag
       61 agctttttat gctcttccag aaatgaataa taaagaaggg ctaaagacca atgcaatata
      121 tggatttaca acaatgctat ttaaaatgat agatatatat aaaccaacac atataagtgt
      181 agcatttgac agaaaagcac ctacatttag acatttagaa tataaagagt ataaggctgg
      241 aagaaaggga atgccagatg aactggcaga acaattacag ccattaaaag accttttaga
      301 taaatttaga ataaatagat tggaaataga tggctatgag gcagatgata ttataggtac
      361 tgtttctaaa aaagcagaag aaaatggata taaggtatac atagtaactg gagacaaaga
      421 tgctattcaa cttgcttcag acaatacaac tactcttata acaaaaaaag gagttggaga
      481 agttgaagaa tacaacttta attctgtaat tgaaaaatac gagatgactc caacacaatt
      541 tatcgattta aagggtctta tgggagacaa gtctgataat atacctggag tacctggaat
      601 aggtgaaaaa acaggtataa agcttataaa agagttttct agtatagaga atctaataga
      661 gaatacagat aagcttaaag gtagtgtaaa aaagaaaata gaggaaaaca aagaaatagc
      721 tattcaaagt aaaagacttg caacaattat aagagatgtg ccaatagaag tggatttaga
      781 tagtatgatt tttggcgatt atgataaaga tgaactttta gataaattta gatactttgg
      841 atttacaagt ctcttatcaa gattagttga tttggtcgat tcagatgaca cagaacacgt
      901 agaagaaaaa atagaaatat taaaattaaa agatatagaa aaatttatag atgaagtaaa
      961 caaaaaagga caagttattt taaaaactgt tagaggacaa ggtaatatac ttgaaaaaaa
     1021 tataatatat atttttataa gtgtagatgg agaaaaaata tattatgtag attcaaatga
     1081 agtgtcaaag ttagatagta tattttcaaa taatgaaatt aaaaagtatg gatataattt
     1141 aaaagatgat tatatatctt taaaaccata taatataaac ttagaaaata tgtattttga
     1201 tataacaata gctgagtact taatagattc aacttcatct acctcatatg attgtagtgc
     1261 aatagctatg aagtatttat caaaaaaggt taaatcaaaa gaagaattat taggaaaggg
     1321 tgttaaatct aaaaactatg aggatttaga atttgaggat ttagcagagt atatgggtca
     1381 aataatatgc acagttaaag aaacagttcc tatgatggaa agaagtctaa tggatatgag
     1441 tatggatggt cttttttatc atgtagagat gcctttagta gaagtcttag gtcatatgga
     1501 gtatgaaggt ataaaagtag acaaaagtat attggatgaa ttgagtgttg aatttaaaga
     1561 aataatagct acattagaaa aggaaattta tgaattatca ggagagccat tcaatataaa
     1621 ttcaccaaaa caattgggtg ttatattatt tgaaaaatta gagcttccaa taattaaaaa
     1681 aactaaaact ggatattcaa ctaatgcaga tgttttagaa aaattaagag ataagcatcc
     1741 aataatagat aagataactg agtatagaca aatagtaaag ttaaattcta cttacgttga
     1801 aggactttta aatataataa atccagaaag taatagaata cattcatcat ttaatcaaac
     1861 tataacgact acaggaagaa tttcatctac agaaccaaat cttcaaaata tacctataaa
     1921 aatggaaatg ggtagaaaaa taagaaaagt gtttattgca gatgataact gtaagttggt
     1981 tgatgccgat tactcacaag tagagctaag agttcttgca catatgagtc aggatgaaaa
     2041 tatgatagaa gcatttaaac atcatgaaga tattcatacc aaaacagctt cacaagtttt
     2101 taatgttcct atggaagatg ttactagtga attgagaggt gcagctaaag ctgtaaactt
     2161 tggtattata tatggtaaaa gtgactttgg tctagctgat gatttaaata tacctgttcc
     2221 aaaggcgaaa gaatatatcg aaagttattt tgctaaatac cataaaataa aagagtttat
     2281 ggacacaaca gtagaaaagg ctagtgagga tggatatgct gtaacaattt taaacagaag
     2341 aagatatata ccagaaataa agtcaagcaa ttttatggaa agaaatagag gaaagagatt
     2401 tgcaatgaat gcaccaatac aaggaagtgc agcagatata ataaaaattg ctatgataaa
     2461 tgttcataaa aaattggaag agaacaattt gaaatcaaaa cttatactac aagttcacga
     2521 cgagttgata gttgaagcaa ttgatgatga aatagatata gttaagaaaa tagtaaaaga
     2581 agaaatggaa aatgctgtaa atatggatgt tcacttagat gtagatttaa atgtaggtag
     2641 ttcttggtat gagacaaagt aggtgatttt atgttgatat taggacttac aggaggaata
     2701 ggctgtggaa aaagcagcct atcaaatata tttagaaatc ttaacattcc tatagtggat
     2761 gctgatatta tatccaggaa gatatttgag gataagttgt tattggaaaa aatatttgtc
     2821 cattttgggc aaagtataaa aaatgatgat ggaactctaa atagaaaagc tcttgggaaa
     2881 atagttttta gtgatgaaga aaaacttaag gaattaaata acttgactca tccacgaata
     2941 agagaaaaaa taataagtga aatagaaaaa ttgagaaaaa aaggcgaaaa tatagtagtg
     3001 ttagatgcgg caatattggt agaaagtggt tttttagata tggttgacaa gctattagta
     3061 gttacttgta aacaagaagt acaaattagt agaatacaaa agagagataa ttgcagtgag
     3121 caggaagcac ttagtaggat aaattcacag atgagtcagg aagaaaaatc caaatatggt
     3181 gattatataa tagataattc gggaacaata actgaattag aaagtaaagc acataaattt
     3241 attgagtaca tgaaggagaa ttggcgtgaa tagtaagaaa gtattgattc tatctatttt
     3301 tataatctta tttggggcac tattaatgga aagcaaagta atacataaat ttttatatcc
     3361 taaaaaatat tcagagtatg tagaaaagta ttcgaaagaa tttaatttag atgaaaatat
     3421 agtttacagt gttattaaag ccgaaagtaa gtttaatagt tctgctgttt caaaaaagga
     3481 agcaaaagga ttaatgcaaa tattagacat aactagagat tggggagcag aggaactaaa
     3541 tttaaaaaat gtggatattt tcgacccaga gactaatata agacttggct gttggtattt
     3601 aagtaagtta tacaaagaat ttggtaaatt agatttagtg atagctgcat ataatggtgg
     3661 ttcaggtaat gtgaaaaaat ggttagaaaa taatgaatat agtaaagatg gcgaaaatct
     3721 acatgatata ccttttaagc aaacttcaaa atatgtagaa aaagtaaaaa ataattacga
     3781 acattataat aagatatatg gcaagaaagg aaaaaactaa tgaagagaat aaaagtctta
     3841 acagtcgttt tagctataac tcttatggta gctggttgta gtaatactaa aacaaaacaa
     3901 agtagtagtg attcaagttt acaaagtcaa agttctatag atgctaagta tataaactta
     3961 acaatggtaa ctccaaaaac tataaatcct ataactaata ctgataagtc tgttggttat
     4021 ataatgaatt tagtttatga tagtttattt actatagatg agaactacaa tgtaattcct
     4081 caattagtta aggagtataa tatagctcag gatggaatga gtatagatat aaagctaaaa
     4141 gatgcaaagt ggcatgatgg aaaaaatgtt acttcaaatg atgtaaacta tactattggt
     4201 ttaattcaaa aaagtgcaga tagtccatat aatgaattta ctaaaaatat agcttctgtg
     4261 aatattaaat cagataaaga ctttacaata aaatttaaag ctagatatgc tttttcaatt
     4321 gatagtttaa tttttccaat tgtatctcaa aatcatttag attcaaaaga tgtaaatgac
     4381 aagaataata atatgatagg aaatgggaaa tataaaatag aatcatatac agaaagagaa
     4441 ggaatggttt tatctgtaaa caaagattac tatgaagaag ttccaaaaac catgaagaac
     4501 ataaaagtag gtatggtgcc taatgaagat gcaaggacat ctatggttat ggctcttgat
     4561 agtgatataa ccaatgttac attaaatgat ttaagtaagt ttcaagaaaa agagtttaat
     4621 ataacaaaat atcaaggaag agattatgag tgtgtattat ttaattataa taatcctttt
     4681 ttcaaagatg ttaattttag aaaagcaatt gcacactcta tagataaaga tagaattatt
     4741 agtgaaggtt atatggatga tgctactcca gttaattttc cacttaattc aaaatcaaaa
     4801 tactataata gtgaaatgaa ggatttagag tttaataaag ataaggcgat agagtgtctt
     4861 gctaaggtag aatatgcaaa tgtaaactca gtaaatcaaa atgacaataa agtaaaaaat
     4921 cagaaaaaat cagctaaaaa gaactcaaag aagcttacgc cagaagaaga agcaaaagct
     4981 aaaaaagcag aagaagatag aatcaaaaaa gaagctgaag agaaaaaaaa tagagaaaaa
     5041 gaggaagtaa agaaaagtct ttctgaaatg aacttaaaga taatagtaaa cagagataat
     5101 agtgagagaa taaaaactgc aaacataata aatgaaaact taaaagctat aggaataaat
     5161 agtacagtaa atcagctgtc agataaggat atggaaaatg ctcttaattc taaaaattat
     5221 gatttagcat tagttggatg gaaactatca attataccag atgcaagtag tattatagcg
     5281 agtagtggat atactgatga taagttaaat ggatatatgt ctgcacttac aagttctacg
     5341 tctgaaattg aaacaaaaaa agcatacaaa gatgtacaga catatataaa agataatgca
     5401 acatttataa gtttagctgt gagaaacaac tatattgtaa gtaatagtag attaaaaggt
     5461 aaaattactc caaatgattt tgatgtatat gagggaatat ctaatttaga tataaaatcg
     5521 aatgaatcaa attaaatgat taatttctat ttaaaaagta taaactatat gatagaatac
     5581 ccttataggg tataaggagg taattaaatg aagaagaaac tattagtaga aggtatgagt
     5641 tgtggtcatt gtgtaaacca tcttaaaact gcattaacag aagatataga tggtattgaa
     5701 gttttagatg tagatttaga aaataagtgt gcatctgtag atatgaaaga tgatgtatct
     5761 attgaaaaat taaaagaagt gatagcagaa ttaggatttg aactaaaggg aattgaatag
     5821 aaattgataa actaaaaaaa gacctctatt gggggagtaa tagaggtcta aaaatggggg
     5881 agattgagta tgtttgattt tacattacta ttatctcccc ttttaaatta atatatacat
     5941 aaaaagtaaa aaaattttgt aatgactcac atttcttgag ctttttgaat atactataaa
     6001 tagctaaggg agggagtatc atggaatcta ggggatataa agaggattta aaaaaaatga
     6061 tacttgatat ggcccacaaa gaacttgaga gttatgtatc acaaaaagga gcaaaaaaat
     6121 actttgaagg taacttggat aagattatta ataataatat aaaaaaattc aatcaaagta
     6181 ttggtcttaa tagagtatat ttaaaaatgt taaaaaagtg tgcatatcaa gaaagtgtaa
     6241 cttcaatatc aaggactata aagaaagaag cattatttaa atcaaaaaaa gagctatttg
     6301 ctttttcaga gtacttaaaa cttgatatta acaagcgact ttcatataat caaattttaa
     6361 gaaaaatatc aagatatata tactacaata gaagttcata tgctcaaaaa tacgttgttt
     6421 tcaaaagagg agatgaagaa tatcttttag aaccagagaa aataaaagat gaattaatat
     6481 caagctatag gtcaaagact agagaagata tgaggtcaat agcaaaactt ttggatatag
     6541 aagtagaaga tgattataat gctgaagata ttagaaaaaa agtaataaat tatattatta
     6601 aagaaaaact taaaagttag agttttaaag aataagttct gccaatttgc atgattaagt
     6661 ataacttggg tataaatttt ttatgcattt gaaagagctt attctttttt tgacatattc
     6721 ttttaaataa tttaccatga tttggtaatt ggtcatcatc attcaaattt ttaacttaat
     6781 atgatttaaa taaatttata ataatgatat aatataaaaa aaataaaaat tggagagtga
     6841 tttcaaaatg aagtttaatt tttgtcataa caatttcaat gttactaatt tagaaaaaag
     6901 tttagatttt tataaaaaag ctttagggct taaagaggta aagcgcaagg aagcagaaga
     6961 tggaagtttt atattagttt atttaggaga tggcattact agtcatacat tagaattaac
     7021 ttggcttaga gattgggata gaccatataa tttaggagac aatgaatttc atctggcttt
     7081 agaagttgag gagtttgatg aggcaaaaaa acttcataaa gatttagatt gcatatgttt
     7141 tgaaaatgaa agtatgggaa tttattttat agcggaccca gataattatt ggattgaaat
     7201 acttcctaaa aatcactaat tttaaggata tgtttataat ttctttacaa ttaataagaa
     7261 aataagttat cattaaattg tagggactaa aaatggataa taatgttatg aatttatgac
     7321 tataaaaatt tgttgggggg agtgatagta gtgaaaaaag ctatagcagc tttaggaatt
     7381 ggggcagtag cagtatctgt tagctccata aatgcaagtg ctcttgaaaa gggaacagta
     7441 acagcaagtg ccttgaatat aagaagtgga ccaagttctg attgcgataa ggtagcaaaa
     7501 ttatacaagg gaaaaactgt agagatttta gaaaagtcta atggatggta taaagtaagg
     7561 gtatcgagtt ctgttgttgg atggggaagt gcaaaataca tatcaacaag tggttcgtct
     7621 gaaggtacat caagtcaaaa taattcaact tcaagtggga caactattag tggaaatggt
     7681 aaagtaaatg tgagttctag actgaatgta agaagtggtg caggtactaa ctattcatta
     7741 gttggtaaag caaataatgg agatgtagta aagttactag aacaaagcaa tggatggtat
     7801 aaaataaaac tatcaaatgg agttactgga tgggctagta gccaatatat ctctaaaaca
     7861 tcagaggatg ttggaacgaa caattctagt aattcaaata gtactaataa ttctgataaa
     7921 aaaccatcta gcgaagaatc aattgaagga aagaatggta aggtaacatc tgctgtgagt
     7981 ttaaatgtaa gaagtggtcc tgggacaagc tattctataa taggaaaatt aaatggtgga
     8041 gatgttgttg aattaaaagc taaaagtaat ggttggtata aggtaaagtt atcgagtgga
     8101 acaattggat gggtgagtgc cagttatatt tctgagacta atgaggatac aaaagaaaaa
     8161 ccaaattcat catctaacca aaattctcaa tctaatagta actcaaagcc atcatttact
     8221 ggaaattcag ataaatctac agcaaaaggt tcaacaatcg tagattttgc atatactctt
     8281 attggaatac catatcaatg gggagcttca gggcctgata aatttgactg ttctggattt
     8341 acacagtatg tttttaaaca ttctgtaggc gtttctattc ctagagtttc tagagaacag
     8401 gctaattttg gaagtgcaat ttctatgggg aattatgcac caggagattt agtttatttt
     8461 gacactgatg gtgatggaac aactaatcat gtaggtatat atgtaggaaa tagtaaattt
     8521 atccattgta gtggtactca gactaatcct aataaggtta aagtagataa tttaactagt
     8581 tcatattggt ctaaagtatt acttggtgca agaagatttg tttagtaagt acaatttctt
     8641 attaaaaaat tagaagaaat aattatttat attcataaat tgtgactctt aaaaatatac
     8701 tttattaata atataatttt gggagggaca aactaatgaa tagaagaaaa acgatataca
     8761 gaggatttaa taataataga cataagattg atttacgtaa atatgttata acgattgctt
     8821 gcttgtgtct aattggctat tattcttata caaaaataaa agatagcaag atattggagt
     8881 atgtatcggc taaaatacca tttctaaata attcatcaga tataacttat aaagatatat
     8941 ctgatgaatt aaattcaata aaaaaaggga aaaaatctaa atcacaaacc aattcagatg
     9001 ataaacaaga aactaatcca gaaaaagcgg taaataacac taaagagcca gaagaagtca
     9061 aattagctac tatagaaggc tgggatatgt atactattca agttgctgca atagataata
     9121 atgatgattt gaaaaaaata cagacatcat tagttaataa tgacatacct ttttcagtta
     9181 tggagaagga tggagtaaaa aaaatacaga cctattcctc ttttgatgaa aatgatgtta
     9241 gaaagcagat aagcagtgta agaaaggtgt ttccagatgc atttttatca cacttagatg
     9301 cacctatgtt gtctttagag tatacaagta actacgctta tatagagagt atatctaaag
     9361 aattaaataa attaataaca aactttaaag aagaatcaag tttttggtcg aatgctgaaa
     9421 ataatgtaga tatggaaaaa tataatacta ttttgacaaa tagaaaggca atatcacaaa
     9481 atatatcaaa agaagctgaa aagattgact attctgaaat gcgtttattt aaagataatt
     9541 taatagagta tgtaaagaat gtgaatgaaa aaattgatac agcttcaaaa gcagcaaatg
     9601 aagaaaaata tagtgtaagt aaaagtctgc ttttatcatc catgcaggag tactctatgt
     9661 ttataaattc tataaaataa aatacagaaa atttggactt tagaaagcta tatgaaaagg
     9721 attgtttaat taagcaatcc ttttcctcgg tttgacttaa tatagaggta ttgatttata
     9781 aaataaatta taaaaatacg gatatttttt taacaattgt taagattttt taatatatat
     9841 agtatactta tagaaggcag aaaaaatgtt tttttataga taagggggta aaacaatgaa
     9901 actcttaact tttaagggag ggatacatcc tccatataga aaagagtact ctaacacaaa
     9961 ggcacttgaa aaagctcaag ctccaaagat agtttacatt cctcttcagc aacatatagg
    10021 ggcaccagct aaacctattg ttgaagttgg tgatgaagta aagtttgggc aaaaaatagg
    10081 tgaacaacaa ggttttgttt catgtaacgt tcattcttca gtatcaggta aagttattgc
    10141 tattgaacaa catgaagtac caggaggttc agctcaatgt gtagttatcg aaaatgactt
    10201 taaggaagaa ttacatgaga gtgttcaacc aaaaggtcag ttagaggatt tgagcaaaga
    10261 agatatagta ggtatcataa aagaggcagg aatagtaggt atgggtggag ctacattccc
    10321 taaccatgta aaagtatctc ctccaccaga tagcaaggca gaagtagtta tattaaatgg
    10381 tgcagaatgt gaaccatact taactgcaga ccacagatta atggttgaaa atccagaaga
    10441 tgttgtgttt ggtttaagag cattaatgaa ggtattagat gtcaaaaaag gatttatagg
    10501 aattgaaacg aataaaccag atgctataga agcaattcaa aatgtagcta aagactatag
    10561 cgaaatcgaa gttgttggcc ttcaagtgaa atatccacaa ggagcagaga aacaacttat
    10621 ttatgcgtgt acaggcaaag aagttccatc tggtggatta ccaatagcag caggtgcagt
    10681 tgttgacaac gttgccacag cagcacagat agctaagtct attaaaacag gtatgccttt
    10741 ggttgaaaga atcactacaa taacaggtag ttgcatcaaa gagccaaaga acctaataac
    10801 aaaagttgga acattagttt cagaaataat agaacaatgt ggtggattta aagaagataa
    10861 gaagattggt aaggttataa tgggtggacc tatgatggga atagctcaat atactactga
    10921 aatagcgact aacaaaggtt cttcaggaat tttatgtttg gatgaggaag aatcacgtac
    10981 tccagatatt caaaactgtt taagatgtgg aagatgtaca gatgtatgtc catcattctt
    11041 acaaccactt ttcataagtg catattcttt aaaagatgac tatgacacag ctgaatatca
    11101 tagagcaatg gattgtatag aatgtgggtc ttgctcattt atttgtccag caagaagacc
    11161 attacttcaa tctataagat cagcaaagag agaaatagga gcaaagagaa gaaagcaagc
    11221 tgctcagaaa taaagaaagt taagggggaa aaacgatgga aaataagttg atagtatcat
    11281 cttctcctca tgtgagaagt aatgaagata cttcatatat aatgaaacaa gttattatag
    11341 cactccttcc agcagcagta gcaggagtat acttctttag acttaatgct ttgagtgcta
    11401 tgtttttttg tatacttggt acagtaggta ctgaattttt atatcaaaaa tttacgaagc
    11461 aaaaaagtac tataggagat ttttcagctg ttgtaacagg attattatta gcatttaacg
    11521 taccagcatc acttccttgg tggatgtgtc tagtaggagg aatatttgca atattagtag
    11581 ttaaaatggt atttggtgga attggatgta actttgtcaa tccagcactt gctgcaagag
    11641 catttttatt agcatcattc ccagtagcaa tgactgcttg gactcaacca ggtgttaact
    11701 ggataggtaa aaatttagat gcagttacta cagcaactcc attaagcttt ttgaaaaatg
    11761 gagcagcagg actggcagac ctttctagca atggaattag tcttgctgac atgatgatag
    11821 ggaatattgg tggatgtata ggtgagacat cagcaatatt attattatta ggtggagtat
    11881 acctaatgta taaaggcata ataaattatg ttataccagt attttacata gcaactgtat
    11941 ttatattaac attcctttta ggtggattta atataacttt cgcaatatat caactgtttg
    12001 ctggaggact tatgttaggt ggattcttca tgcttacgga ttatacaact tcgcctatga
    12061 ctaaaaaagg tcaaataata tacgctgtat tagctggtct tataacaact gttataagaa
    12121 tgtatggtgg atatccagaa ggtgtatctt actcaatact attagtgaac tgtcttgctc
    12181 cacttataga taagtttgtt agaaatagag tatttgggga ggtggctaaa taatgaacag
    12241 tatggtgaga ttaggtggta ctttacttgc tataagtgca atagctgcat tagctcttgg
    12301 agctactaat caggttacag ctccagtaat agaacaaaga aatatacaag caaataatga
    12361 acttagaaaa gcagtccttc cagaagcaaa agaatttaaa gaaatgaagg aagatactta
    12421 caaaggcctt ggagatggtc ttatagctga agtgtatgaa ggtttagatg gttctgaagt
    12481 ggttggatat actttaaaag ctaaaccaag tggatatggt ggagaaattg aagttatggt
    12541 aggtatatca tctgaaggtc aagttacagg tgttgacatt ggtaatatgt cagaaacagc
    12601 aggtcttgga gcaaaagcta aggatgatgc atttaagggt caatacaaag gaaaaactgc
    12661 tgagcctctt gaagtagcaa aaggaagtac aacagcagat aatcaaatat tagcaatatc
    12721 aggtgcaact attacatcta cagcagttac tacaggagta aatgctgcta tagatgtttt
    12781 caatagtgct cttaataaat aaggagggtt aattatgaat ttagctaaag tatttaaaaa
    12841 tgggctaata gatgagaacc caacatttgt tcaagttata ggtatgtgtc caacactagc
    12901 cgttacaact tcagcaataa atggaattgg tatgggtctt tcaacagcag cagttttaat
    12961 atgtgcaaat ttagttatat ctttaataag aaaaattaca ccagataaga taagaatacc
    13021 tatatttata gtagttatag ctacatttgt tacaatagta ggtatgcttt taaaagcata
    13081 tgttcctgct cttgataagg ctcttggtat atatatacca ttaatagttg ttaactgttt
    13141 aatattagct cgtgcagaaa gttttgcttt caaaacagga gctatgcctt caatagttga
    13201 tggtgtaggt caaggtcttg gatttactgt tgcacttaca ataataggtg cagtaagaga
    13261 attacttgga aatggtagtt tatttggaat gacattattt ggagcatctt tccaaccagt
    13321 attaatattt atattaccac caggtgcatt cttaacatta ggattcttat ttgcaggatt
    13381 taataaatta agaagtaaaa aagcatagtg gaggtgtaat aaatgaattt aatactttta
    13441 tttttaagta tcgttcttgt taataacgtt ataacatctc aattcttggg tatatgtcct
    13501 ttcttgggag tttctaagaa ggttgataca gcagtaggga tgggagttgc agttacattc
    13561 gttttaactc tagcttcaat tataacttat tttatacaga ttttattagt aaaaactgga
    13621 actggattct tacaaacaat agcttttata ttggttatag cttctatagt tcagttcgtt
    13681 gaaatggtaa ttcaaaagat gagtccatct ttatatcaag cattaggagt ttatttacct
    13741 cttataacta caaactgtgc agttcttggt atagctctag ttaatgttca aaaaagttat
    13801 aatttagtag aaacaataat aaacggattt ggagccggag ctggatttac tttagcaata
    13861 gtaatattcg caggtataag agaaagacta gaattagctg atataccaga agcatttaaa
    13921 ggtttcccta taacgcttat atcagcaggt ttaatgtcaa tagctttctt aggatttaca
    13981 ggacttataa agctataaaa aagaggggtg aaaatggtga tacttacagc tgtattagtt
    14041 ttaggaatta tgggcttaat cttcgggata gtactagatt ttgcatctaa aaagtttgct
    14101 gtagaagtag acgagagagt ggaagctata ctaggagtac ttccaggagc caactgtgga
    14161 ggttgtggat ttccaggttg tggaggtctt gcaaatgcga tagttgaagg caatgcacca
    14221 gttaatggtt gccctgtagg tggagcagat gttggtgcaa aagttggaga aataatggga
    14281 ataagtgctg aagcaggtga gaaacaagta gcaaaagtta tttgtaaagg aacttgttca
    14341 agtgcaaaag ataaatatga gtatgaagga atatcagatt gtagagcagc aaatgtttta
    14401 aactcaggtg ctaagatgtg taaatttgga tgtttaggtc ttggaacatg taaagatgct
    14461 tgtaaatttg atgctatttc tatagttgat ggaatagcag taatagatga agaaaagtgt
    14521 gtaaattgtg gaaaatgtaa agaagtttgt cctaagggta taataatcac aaaaccagaa
    14581 agtcaagaag tagttgtaga gtgtaatagc aaagagtttg gtaaagctgt taaagaaaaa
    14641 tgtactgctg gatgtattgg ctgtggaatg tgtgtaaaag cttgtaaatt tgatgcaata
    14701 atatttgaag acaaaatagc taagatagac cctaataagt gtgtaggttg tatgcaatgt
    14761 gtagcaaaat gccctacaaa agtaatatca ggtgacataa ctaagaaaaa gaaagttact
    14821 attgaccaag aactttgtgt tggatgtaca gtgtgcaaaa aacaatgtaa gtttgatgca
    14881 atagagggag aattaaaaga aaaacataag gtagatgcag ataaatgtgt aggatgccat
    14941 ttatgtatgg aaaaatgtcc aaagaaagct attaaaattt tgtaatatgg acaaaataag
    15001 gatatggaat aatttccata tctctaatga gtacataaaa cagaggtatg gaataatttc
    15061 catacctcta ttttatgtat atttattcta atggtaataa atataacaat agtgtaacaa
    15121 aaaaggttcg acatttctgt tgaatttttt acaatatagt gatagactaa ataggtacaa
    15181 attacgtaca aatgaggtga ataaatgaat ataatcttgg catcagcctc tccaagaagg
    15241 aaagaaattc tagagaatac gaatgtaaga tttgatataa ttaaaaatga aattgatgag
    15301 attatattgg aaggtgaaac accaaaacat ttagttatga gattagcttt tgaaaaaagt
    15361 atgtctgtag catctgaaca taatgaagat gttgttattg gagctgatac agttgttgtt
    15421 ttagacaatg ctattttagg aaaaccaaaa gatgagtctt gtgcaagaga tatgttaaaa
    15481 agactatctg gaagagagca tcaagtcata acaggtatta gcttaataaa cttgtgcgaa
    15541 gataaaaagg taattgacta tgtaatcagc aatgtgaaat ttaaaacgtt atcagagcag
    15601 gatattgagg attatctgaa aactaatgag tcatttgaca aagcaggagc ctatggtata
    15661 cagggttatg gagctttgtt agttgaagaa attagaggag attattttaa tatagttggt
    15721 cttccaattt caaggttagg cgacctatta aaaaaatatt ttagtataaa tcttttttat
    15781 ggagtgtgat ttatctgaaa aagtcattta attcaaccat taaggtaaaa gaaatggcac
    15841 cagaggaaag acccagagaa aagatgcttg ccaaaggcgt aaaaagctta tcaaatgctg
    15901 agttgttggc tatactttta agaactggca acaagaataa aaatgctatt gaattagcca
    15961 actatataat aaatagagat attcagggta ttagacattt agaagatatg acaatagagg
    16021 agctgtgtaa tatagatgga attgggttat caaaatcaac ccaaattaaa gcagctttag
    16081 aactaggctc tagagtagct agttttaaac ctataaagta taaaataagg aatccttggg
    16141 atatacagag gtattatatg gatagcctaa ggtatttaaa aaaagaggtt tttaaagcgg
    16201 ttcttttaaa cacaaaaaat gagataatat ctgatgtaga tgtatctata gggactttaa
    16261 gctcgtcatt agtccatcca agagaggttt ttaaagaagc tataagaaga agtgcaagta
    16321 agataatagt gatgcataat cacccttcag gaagtgtaga accatcaaga gaagataaaa
    16381 atatcacatc aagacttatt aaatgtggag aaataattgg aatagaaata atagaccata
    16441 taattattgg agatggatta tactttagct ttaaagaaaa tatgataatt tgactttata
    16501 tgtgataaaa taataggaga atgttttaaa catgattctt aatagtagga aggagtaaaa
    16561 tcatggctaa ggaaaaaaag aaagaaaaaa aaggtttttt tagttttaat aagatgacaa
    16621 aggatatggg aatagattta gggactgcaa atacattagt ttacataaaa ggacaaggta
    16681 ttgttgttag agaaccatca gttgtcgcaa taagagatga tagtaaagag gtattagcag
    16741 taggtgaaga agccaaaaaa atgataggta gaacaccagg aaatatagta gctataagac
    16801 ctatgaaaga tggagtaata gctgattttg acataactca atcaatgatt agctatttta
    16861 tacaaaaagc ggctgataaa aaaggtgttg taagtccaag aatagctata tgtgttcctt
    16921 ttggagttac agaagtagaa aagagagcta tagaagaggc tgcaagacaa gcaggagcta
    16981 aagatgcatt ccttatagaa gagccaatgg cagcggctat aggggctgga ttaaaagttg
    17041 aagaaccaga aggtaatatg gtagttgata ttggtggagg tacttcagaa atagctgtaa
    17101 tatctttagg aggaatcgtt actgctaaat ctataagaat aggtggagac gaatttgacg
    17161 agtctatagt ggcttatgta aaaaaagaat ataatcttat gataggtgaa agaactgctg
    17221 aaaatgtaaa aattaacata ggttctacat ttaaagatga tgaagaaata aatatgcaaa
    17281 taagaggtag agatttaatc tcaggattac ctaaaacaat agaaatatgc tctacagaag
    17341 ttagagaagc attaaaagaa cctgtaagtt caatagtaga tgcaataaaa tctacactag
    17401 aaagaactcc accagaatta gcctcagata taatggaaaa cggaataatg ctaacaggag
    17461 gaggcgcttt gcttagaggt cttgataagc ttattacaca ggaaactggt atgtctgtac
    17521 aaatagcaga aacacctctt gattgtgttg cacttggaac aggtaaatct gttgaagacc
    17581 aagaaatctt tgaaaaagta ttaatgatga atactaagaa ttagaagttg gtgatggcct
    17641 tgagatttga caagaacaaa aatgagaaaa aaaggattaa tgttaaagtg atagcaacgg
    17701 gcgtagttgc tatcacttta attggaattg tgggtatatc aattggtaaa ttttctagtg
    17761 gtagtccagt aaatttagga gtagcttcag atgctattac aagtgtaggt aagggaataa
    17821 atgatggatt ttcttttatc aaaaatggtt ttaaagatgt agcaaatttt aaagataatt
    17881 ctaaaaaggt aaaaaaatta gaagaagaaa atgaaaaact taaaaaaaat atgatagctt
    17941 taaatgccaa actagataaa acagaatcgc tagaagagtt aaaaaagaca ctgaactttg
    18001 tacaagaaga gtataaggcg acaagtattt cagcaagtgt tgttggtaaa aatgatggaa
    18061 actggtatga gagttttgtc ataggtgcag gaaaaaattc aggtgtaaaa aaagaaagta
    18121 tagttatgaa tggtagtgga ttagttggca tagtttatga ggtttccaac aattatagta
    18181 aggcaatttc actattggat tctaaggcat ctgtaagctt taaactagca aaggatgcca
    18241 atgctaaagg tacaattact caaaatacta ctttagataa taaagatagt tataatagta
    18301 aaggatattt gcaaggatat atgtttgact catcatataa tgtaattcaa ggagatataa
    18361 taacaacatc aggtcttgga ttcttcccag atggtatacc aattggcgaa gttgaaaaag
    18421 tggttgatga taaagataag tcattaaaat atgtagttgt aaaaccttat gttgatttta
    18481 agaatataaa tgacgttgta gtcatagaac caagaaatat aggataaagg ggataaaaac
    18541 aatgaaaaaa gttttacttt gtctgttggg gattgtgctt atgactatag aaaatagtat
    18601 aacaaattat atagatattt ttggcataag ctttaattta gttcttatat atgtaaccat
    18661 aatctctctt tatttagatg aattagaagg tgggattata gcagcaataa tagggcttat
    18721 taaagacata accgttggtg gaatttttgg tgtaaatgca ttaattttgt ttattattgc
    18781 atatgcaatt ggatacatga gagatcagct atataaaggt agttatatta ttactttcgt
    18841 tttaattttt ataggcagtt taattgattc aattgtcaat ataggtacat ctagtataat
    18901 ttatcaaagc tacaacatat caacactttt tgtaaaaggt ctactagtag caccaatagt
    18961 gaatagttta attggtttag taatatatcg cttatctaaa agagcagttt taaaacttaa
    19021 agaagattag ttggtgattt catggaagaa aataaacaag ttgatagact aaaaataatt
    19081 aagatagtca tgggcgtgat atttttagct attttagtaa aaataatcta tatgactaca
    19141 tttaaatatg aatattacaa tgagttagct gagaataaaa catataaaaa gttggcaata
    19201 gaagctccaa gaggagaaat aaaagataga tatggaagac tgttggcagg aaataaaaac
    19261 ttatttactg ttcaagtttc aggtaacgat attaataaga aagatgcaaa taagcattca
    19321 agagcaaatg aaatatcatt aaaacttata aatttgctgg agagaaatgg tgaagaatat
    19381 gtagatgagt ttcctatata tgtagaaaat ggaaagtact attatacata tgacagagat
    19441 attagggaat ataaatcaga aaatggtatt ccaaatgatt ataatgctaa ggaaagtttt
    19501 tattacttgg tggataaact tatatcagct ggaatattat ctcaagaaga taaaagattg
    19561 gatgcgacaa gattacaggc aaaactaaat gaaaatggat attatccacc tattttagtt
    19621 agtaaatgga tgttcacagc agaaagagat aagagagatt ggttggctag ttataagata
    19681 aaagaaacta aactgagtgc caaagaagcg tttgaaaaag taagaaatag tgatgcatta
    19741 gaaattgata aaagtttgag tgatgaagat gcaagaaaaa taatggtcgt aagggatttg
    19801 ataaaatcaa aaggatattc tcaatataat ccagtaacta ttgctaagga tgtaggagaa
    19861 actacgattg ctcaaattga agaaagtgct atggaccttg ttggtgtttc aatagcagta
    19921 gaaccagtaa gatattatcc taatgggtca ttagcttctc atatgttagg atatgtagga
    19981 aaaatgccat ccactcaaat agaatcatac cttcaaaaag gttatgaaac tggagacatg
    20041 gtaggtcttg caggagtaga aaagagtaat gaaagtagat taagaggaac tgatggttat
    20101 aaaatggtta aagttgatgc attgggtaga atcagtaaag aaattgaatc taaaaaacct
    20161 aaatcaggag atactgtgta tcttacacta gataaagatt tacaagaagt ttcagacaat
    20221 gcattaaaac agataatcga agttgcttct aaaggaggta cttttaaaag taaatttggg
    20281 gacaaaccaa ttagtgcata tgcaggaaaa gcacagtcag cagctcttat agctattgat
    20341 gttaaaaatg gagaagtatt agcttcatca agttatccta attatgaccc aaataaattt
    20401 gcaaaaggta tttctacaga ggattataaa gcacttcaac caaaaaatcc aaatgactta
    20461 ctagcaggaa gtccactttt aaacttggtt acacaagggg aatttcagcc aggttcttca
    20521 tttaagatgg taacttcaat ggcagcgtta gaaaatggtc tggatccaaa ctttactata
    20581 aatgaccctg gggttataat gttaggtaaa aaatcttttg gtgactatgt atggaaccat
    20641 ggtagaggaa accatggtat gacaaactta tataaagcaa tacaagagtc ttgtaatata
    20701 tatatggcaa caattggtac aggtaaaaca tggccagatg gaaaaagcat tggtatagat
    20761 atgaatgcaa ataaaatact tgagtatgca aaattatttg ggcttgacca aaatacaggg
    20821 cttcaagatg aagtagaaga aagagcaggt aaagttccta gcacagaaga taaattgaaa
    20881 tcaacacaag ctcttctaaa gtcaaattta gaaagagaaa tggcaaatga ttttgttgat
    20941 ataactagag aaaaaaatcc taaagaatat gagaaaagaa tcaatgagat tgtaagctgg
    21001 gcagcagaaa agaaaactcc tggtagagtt gaaacaatga ataggctaaa aaaattggat
    21061 gttaaagaag atagaattga agaagtagca gatttagcag tatttagtta ttttaacttt
    21121 gcaaaatgga gtactgcaga tacatttaac ttagctatag gtcaaggtga aaatgcttat
    21181 acaccagctc aaatatcaag atatgtagct gctatagcaa atggaggtaa tttagtagaa
    21241 ttatctgtag ttgatagagc tgtttcaagt gattattcat cagttaaaat taatgaccag
    21301 aagaaagtcg aaaaaatacc ttttaaaaat ccagataact taaaagaatt aacaaaaggt
    21361 atgaaattgg ttgcaagaca aggtacagcg aagagtgctt ttgctgattt tccaatagat
    21421 gtagcagcaa aaactggtac agctgagaaa agtgggaaaa tacctacaga taacgagtat
    21481 gaatatttaa aaagtcatat gtcatcttat aatgtcaacc taaatgatgc gattaaacta
    21541 gctgataaga tgaaagcaga aaaagaaaaa gagctttcat tagctaagga aaaggaaata
    21601 aaaaagaaat tagaaaataa agatttgaaa gacgaagaaa gaaagaaatt agaggaagaa
    21661 ttagaagatg gagtaaaagt tagattagag gacactgata aggtgaattc ttcatatcta
    21721 agaaaagcta taaaagaatt aaatccaaaa ataacagatg accaaataga tagatttaaa
    21781 caagattatg gttcttttac gtggactgtt gcttttgctc cagcagatga cccagaaata
    21841 gctgttgtat gtgtaattcc tcaaggtgat tcaagtgtat tctcactttt accaactaga
    21901 gaagttattg gaacttatat gggattagag ccaactaata gtaaaaatga caacaagacg
    21961 aatgatgtta acaattcttc tgacgaaaat ataaatttcg agtcacaaat taatagatag
    22021 ctcaatctat gtataaactt tagctttata tagaaaaaat aaaagggttt aaagcgtatt
    22081 tgaagaatat aaatgatata tcactctgga ggttgttatg tctttaagag aaatttgtag
    22141 tcaagaacta gtagagttca agggtaataa aagagggata atagtaaata taaaaagaga
    22201 agctccattt gaggaaatac aggaaaaaat aataaacaaa ttggaagcat atgttggctt
    22261 ttttaatggg gctaaaatta gtaaaattaa tagtgattgt ttaactgaca tggagattct
    22321 agaacttaaa gaaggaatta cttctagatt tgatgtggaa tttgttgaag atcaaaaaat
    22381 tgaagaaaat agtaattttc caacaaaata tgtcaatacc ctaagatctg gtgaaaatat
    22441 tgaatttgaa ggtgatgtag ttatattgaa tgatatgaaa cctggttcaa aagtcctttc
    22501 aaaatcaaat acagttgtta tgggagatat aaatgctggt gctaaggttg tagcaggagg
    22561 taatgttttt gtaatgggta aaatagaagg ttttgtacat gcaggtgcag aaggcaatga
    22621 atttgcatat gttgttgctg gaaatcttaa tcctaaaata ttacaaatag cagataatat
    22681 tgcagaagct ccagatgatg aagaaaatta tgaaagtgaa tcagaaatta gcccagaaat
    22741 agcctttgta agtaatggaa gaattgtaat cgaaagctat ttgtcaaagt tagacaaata
    22801 gaaagaaaaa tagggggtag tattatgagc gaagttatag ttataacatc tggtaaaggt
    22861 ggggttggaa aaactactac cgcagcaaac ttaggaactg cgctaagcct agaaaataaa
    22921 aaaacagtag ttgtggatgc agatattgga cttagaaact tagatgtagt aatgggtctt
    22981 gaaaatcgaa tagtttatga catagtagat gtagttgaag gaacttgtag gcttaagcaa
    23041 gctctaataa aagacaaaag atttgataat ctatatttat taccagcagc acaaactaga
    23101 gataaaaatg ctgtctcagt tgagcagatg attgacctgt gtgagaaact aaaagaatct
    23161 ttcgagtata taataataga ctgtccagca ggtattgagc aaggctttaa aaatgcagta
    23221 gctggagcag atagagctat tgtagttact aatccagaaa tatcagcagt aagagatgct
    23281 gatagaataa taggtctatt agaagcaaat gaaataaaag agataagatt agttataaat
    23341 agaattagaa atgatatggt taagcgtgga gacatgatgg ataaacaaga tataatagaa
    23401 atattagcaa tagatttgtt aggtcttgtt cctgatgatg aaagcataat tatatcaaca
    23461 aataaaggag agccagctat acttgattct aagtcacttg ctggtcaagc atacaaaaat
    23521 atcgcaaaaa ggatactaaa tgaagaagtt cctctacttg atcttgaagt tgaagatgga
    23581 ttctttggta gacttaaaaa aatgtttagc atggctaagt agcaaaggag gatacatctg
    23641 tgttagattt atttagagtt ttttctaacg aggctaaaac tagtaaatct gttgctaaag
    23701 agaggttaaa gctagtttta gttcatgata gagtagattg ttcaccacaa cttttagaga
    23761 tgataaaaac agacattttg aaagtaatag ctaattatgc agaaatagaa gatgatggtc
    23821 ttgaaataaa aatgtctaaa tgcagaggtg agcatgatga caaacctgta tctgcattag
    23881 ttgctaatat accactaaag aatataaaag atagatgtat gtaattagtg cttaaaaggg
    23941 ggatatcctt attggtatat gcccctttta tttaattata tgtttaaaga tagaaagacg
    24001 gtgattcttt gaaaaaaatc aatattaatt ataagtcgac aattaagttg ataaaacagt
    24061 tagactggaa acttatagtt acagtattag ctatctttat ttttgggctt gttatactta
    24121 gtagtgcaac acatgctaat tcaacaggtt catataatca attgattaaa caaggattag
    24181 cttttgtact tgggatagga atgattatag tcatattatt ttttgactat aaccttctag
    24241 gcaggtatta taaagcgctt tatataataa gtcttatttt gttagccata gttttattac
    24301 ctggaattgg gacagtaaaa ggtggagcta ggtcgtggat taacttagga ccacttgact
    24361 tgcagacatc tgaaatagtc aaattaacat ttgtactaag ttatgcaaag atacttgaat
    24421 ccaaaaagga taaattaaat acgttaaagg aagttatgcc tgttgtagtt tattctcttc
    24481 catttatcgg attattgatt gcacaaccag acttgggaac aggaattgta ttttgttgta
    24541 tgatatttgc aatgctgttc acagcaggtc ttagctcaaa gttgataaaa agaggaataa
    24601 tcatactatt agtgtcaatg ccactgatgt atttaatgat ggcagaccat caaaaggtaa
    24661 gaatagaagc ttttctaaat ccagaagatg taactctaaa aggaaactat caagttatgc
    24721 aatctctgat tgcaattggt tctggaggcg taactggtaa aggactttac aatggaagtc
    24781 aaaatcaaga agatttttta cctgtacaag atagtgattt tatatttgct gttgtaggtg
    24841 aagaacttgg tgtaatagga atggcagtgt tgataatctt atttatgata ttcctattaa
    24901 ggcttttagc aattgcaaga gatgcaaaag atttttatgg aaccctcata gtggttggtg
    24961 ttatgggaat gtttggttac cagataatcc aaaatatagg tatgacggtt gcgttaatac
    25021 cagtaactgg agtaacatta ccttttgtaa gttatggagg aagttcgtta ttgacatctt
    25081 tagcaaactt aggattagta ctaaatgtgt gtatgagaag aaagaaaata aatttctaaa
    25141 tttaaataca tctaaacata aattgaggag attagatata tgaatatagc attagtagca
    25201 catgaccaaa tgaaaaacac tatggtcgga ttttgtatag gttatgaatc aattttaaaa
    25261 aagtatggac tatatgcaac tggaactaca ggaaaaagaa taatggatga aactgaatta
    25321 aatataaata gattagcatc tggtccttta ggtggagacc aacaaatagg ttctttaata
    25381 gtaacacaag aaatagattt agttattttt ttaagagacc cattgacatc tcaggctcat
    25441 gaaacagata tacaagcgtt aataagactt tgtgatgttt atcatgttcc aatagctaca
    25501 aatctagctt cagcagaaat atttataaaa gctcttgata gaggagaact atcatggaga
    25561 gaagttagaa aaagcaaaag tcaacgtatt taaaaaatat taatattaca gtctaaagaa
    25621 acagatatat aagtgtttaa tgccctagtc tttctgttgg aataaaccta gtacctgtag
    25681 aaatcttatg aaagatttaa atgatgaaga gctaaatttg agattaaata attaggaaat
    25741 agattataga tagagtagta gaatttaact attactctat tttaataata tgaaatagaa
    25801 tcaagtctat tatta
//
LOCUS       DAKBNN010000033        25461 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000033 DAKBNN010000000
VERSION     DAKBNN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.033"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            122..1087
                     /locus_tag="OUB02_001220"
     CDS             122..1087
                     /locus_tag="OUB02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCT8884991.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(1285..3396)
                     /locus_tag="OUB02_001221"
     CDS             complement(1285..3396)
                     /locus_tag="OUB02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HCT8884992.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(3698..4405)
                     /locus_tag="OUB02_001222"
     CDS             complement(3698..4405)
                     /locus_tag="OUB02_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT8884993.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            complement(4604..6517)
                     /locus_tag="OUB02_001223"
     CDS             complement(4604..6517)
                     /locus_tag="OUB02_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCT8884994.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(6601..7692)
                     /locus_tag="OUB02_001224"
     CDS             complement(6601..7692)
                     /locus_tag="OUB02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT8884995.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(7698..9065)
                     /locus_tag="OUB02_001225"
     CDS             complement(7698..9065)
                     /locus_tag="OUB02_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HCT8884996.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(9019..9771)
                     /locus_tag="OUB02_001226"
     CDS             complement(9019..9771)
                     /locus_tag="OUB02_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8884997.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(9819..10727)
                     /locus_tag="OUB02_001227"
     CDS             complement(9819..10727)
                     /locus_tag="OUB02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8884998.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(10749..11498)
                     /locus_tag="OUB02_001228"
     CDS             complement(10749..11498)
                     /locus_tag="OUB02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8884999.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(11527..12702)
                     /locus_tag="OUB02_001229"
     CDS             complement(11527..12702)
                     /locus_tag="OUB02_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCT8885000.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(12717..13556)
                     /locus_tag="OUB02_001230"
     CDS             complement(12717..13556)
                     /locus_tag="OUB02_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8885001.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(13559..14716)
                     /locus_tag="OUB02_001231"
     CDS             complement(13559..14716)
                     /locus_tag="OUB02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HCT8885002.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGKVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(14732..15454)
                     /locus_tag="OUB02_001232"
     CDS             complement(14732..15454)
                     /locus_tag="OUB02_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCT8885003.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDLNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(15466..16539)
                     /locus_tag="OUB02_001233"
     CDS             complement(15466..16539)
                     /locus_tag="OUB02_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HCT8885004.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(16561..18267)
                     /locus_tag="OUB02_001234"
     CDS             complement(16561..18267)
                     /locus_tag="OUB02_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCT8885005.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFKEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDSVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(18313..19869)
                     /gene="murJ"
                     /locus_tag="OUB02_001235"
     CDS             complement(18313..19869)
                     /gene="murJ"
                     /locus_tag="OUB02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCT8885006.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(19984..21039)
                     /locus_tag="OUB02_001236"
     CDS             complement(19984..21039)
                     /locus_tag="OUB02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HCT8885007.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(21134..21820)
                     /locus_tag="OUB02_001237"
     CDS             complement(21134..21820)
                     /locus_tag="OUB02_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCT8885008.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(22040..24064)
                     /locus_tag="OUB02_001238"
     CDS             complement(22040..24064)
                     /locus_tag="OUB02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8885009.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(24318..25430)
                     /locus_tag="OUB02_001239"
     CDS             complement(24318..25430)
                     /locus_tag="OUB02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HCT8885010.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         7947 a         4634 c         2757 g        10123 t
ORIGIN      
        1 aattatatca ttgtcttttt tttgtaactg aaacaagtat tgaatatatc tcaatacttt
       61 aatataatta agtgtatgct attaaaaaat atacaggaaa aagaagaaag aaggggtact
      121 tttgtcaaaa ttaaagaaat ttgttatact actggctttc ttggtagtaa tattccctat
      181 aagtgtctat ggatattttt attataaatt atctgctatt catgactcga gcataagtag
      241 tgatttattg gataacaatg accataaaaa tgaagatggg ataattaaca tactacttat
      301 gggaacagat gctagaccaa atgaagattc ttcaaggtca gatgctatga tgatacttac
      361 tattgataac aaacacaatg atatcaaact tacatcatta gctcgtgata gctatgtaga
      421 catccctggt catggtaaac aaaaacttac tcatgcttat gcttatggac aagctgattt
      481 attaatacaa acaattgaag agaattttaa tattgatatt caaaattatg cttgtgttaa
      541 tttcgaatca tttatgtata taattgatgc tattggtggc gttgaggtta ctgttgaaaa
      601 aggagaaata cgagaactaa ataaatttat tcctgaaact tataaatgga ataaaagtga
      661 tgataaaggt agcatacaat acattagaaa ctctggaaag cagactctaa atggatatca
      721 ggcactatct tttgcacgta tcagacacaa cgatacagcc tttgctagag atggcagaca
      781 gagacagatt atacaagcta taatcaagaa aacggaaact ttacctgtta ctaaatatcc
      841 aggtttatta gatgcagttc ttccatatgt taaaactaac atgaagccta atgcaatact
      901 ttctttagga gctcaagttt taaaaatggg tgacttaaat attaagcaat ttgaatttcc
      961 tattgatgat gaaattcaca gtacaggtgg tatatatgga aaagctggat gggttcttag
     1021 atttgaccca gatactttag acatattaca cgattttata tttaatgaca tagagtttaa
     1081 acaataaaaa attcaacata aagtttatta aaaagtataa gattaattac taaaaaagaa
     1141 ctatatcgca taattatttt caagttgctg tgtacacttg attacaaaat gcatatagtt
     1201 cttttttatt ttctaattta tcatgataat tttcatttta atctacattt ataaaatact
     1261 ctgtatttta aattaacttt tttattactt aactaagttt aagaaagtgt ttaatgtgtt
     1321 ttgatttata ccatctccag cttcgtatac tagagtagtt ttcttaggct ctaaagcagt
     1381 tttctgagca gatgttacac tatttccaag cataactact ggtgatttta atttagctgc
     1441 taaaggacct gatgttaatg catctaccaa ttgattactt ttacttacaa acattgatga
     1501 atattcagca tttggataga atttttgtat tacttttcca tttgtttctt ctctattttc
     1561 tccagctatt ctatttttag atacatcatt tgtaatgacc ttatccagct tatttattac
     1621 tgaatcagat atacttagat taccacctat aaaataagca tcttttactt ttaaatctga
     1681 tatccaattg taaacttcat cacttacatt atcttttgat actaatacta ttggttgctt
     1741 ttcttctcct gcttttgaag ctatagaaag agaatctgct tcacctgtac cacttgttat
     1801 atatagtttt tctactggat tagtttttac taattctttt gctatcatta aagatgtact
     1861 atatctatct gaaccaccaa ctctatttat tgatacattt gacaatgtat cttttatctc
     1921 actctcaact ttatctccaa tagagttatt tcctccaatt aaaataactt tacttggatt
     1981 aagtcttttt aactcattct tagtggatgt aggtatatta tccttattta ccaatagtat
     2041 aggagcatca tttgatgtag caagtggtgt tgatgttata ccatcaacta tagagtaccc
     2101 atttactaat actactgtat ctgaatttgt agcccaacct tttttactta ttgctactgc
     2161 tgtgtcatat ctttcgtcgc ctcttaattt ctccacttta acattagatg gtggagtaac
     2221 tggttcttca ctactagaat ataaactctt taaatccttt tgtatagaag tactatttgc
     2281 tatatcttta gctgagaaat acatactacc ttttatttca ctatactttc tatttaatgt
     2341 tacttgttgt actatttctt ttgctatatt ttgtccacca taactcgatt gaccttgctt
     2401 atatatacct tgtcctatgt ataagtctac atttgttcct tttacttcat tagcccacca
     2461 tgccactaat ttagaataat ctgctgcttt aagaccaatt ggccagtaaa gttgtggaac
     2521 tacatagtca atcaatcctt gttttatcca tgctcttgta tctgcatatg tacctacata
     2581 actttcatta cctgatgtat ctgaacctgt tgggtcacta ctcttatttc tccatatacc
     2641 tgctgggctt acaccaaata caacattagg ctttatactc tttatagacg cttttacatc
     2701 tctaagtaga gtatttacat tttctcttct ccaattatcc ttattttgtc cttttccata
     2761 tttttggtat gttgcagtat cattgaaact tactcctgga tagaagtaat catcaaaatg
     2821 aactccatct atatcgtaat tttgaactac ttctgctata ctatccacta tgtactttct
     2881 aacttctggt aaacctgggt cgtagtaata cttatttcca tgtttaacta cccagcttgg
     2941 atttttcttt gctggatgat ttgctggcaa tttatctatg ctctcatctg ccatagttat
     3001 tctatatgga ttaaaccatg catgaaattc cattcctctt ttatgtgctt cttctattaa
     3061 aaatggtaat gggtcatatc cagggtcttt accttgtgta ccggtaagat attcagacca
     3121 tgggtttata tttgatttat ataaagcatc tgattttggt cttacttgaa ccactgctgt
     3181 atttattcct acacttttta atttgtccaa taaatctgtg tattcctttt tttgcttggc
     3241 ttcattattc ttagtcttag gccagtctaa attataaact gtagatatcc aagcagctct
     3301 catttcctta tcatttgatg aatcagcaaa tgaacttaca ctacacatag taagtgtcat
     3361 tatcaatgat aatactaata tacttatttt tttcatttca tcccccttaa ttttttaaat
     3421 ttattttttc tacaatatac ttcttattaa gtatatctaa aattcatatt atctgtcaaa
     3481 tattgaataa tatagttaaa atttatattt gatatttgat tataatatga aataatttat
     3541 tataaaaaaa acagcaaaga aatttatctt ctttgctgtt tttataacgt tcatcactat
     3601 tttaatatta ctaaactctt aaatctctat gtaaaacatt aaatgtaatt atatatttaa
     3661 tgttaatatt caatcctatg taaatcaata tctattatta ttgtaaaaca cgtgtagcag
     3721 ttataagtct ttttgcataa ttagttcctg gagccatgct agtaactttt actacatctc
     3781 ctgtttgtgg tgaatgtatg aatttagaat ctcctacata caatccaacg tgatttattg
     3841 aaccacctga accaaagaaa actaaatctc ctggttttaa ttctgctctt gatactttct
     3901 ttcctactgt tgcttgtgat cttgatactc ttggtaaatt aactcctgct ccatttttat
     3961 agacataaga agtgaaccca gaacaatcaa aagaatttgg tccagttgct ccccatttat
     4021 atggcttacc ttgttgctta aatgctaagt ttaaaacatt ttgtaccttt gcatctacat
     4081 ttccaccttg aatagttaaa taatctccat ttacccatcc tgtaacattg tcaacacttt
     4141 ttattttaat ccatccattt gaatgtaatt ctaaaacttc aactttgtct cctgtgttta
     4201 tctttcctac tactgcatca tttgcactag ctccacttct tacatttaaa aaatcacact
     4261 ggtctactgt tgcactcata ggtgaattag ttggctctgc tgcactagcc gttattgctc
     4321 ctgcttctac tgtgacaact cctgctatta acaccatagc tataaatttt ttcaatatat
     4381 gttttttatt tttaatattc atcataatct caaataccta atcctttcga aaatttaaca
     4441 ttttgttcct tcgtttccaa ttcctaatta acattctatc ttacatcaaa ataaattgca
     4501 accttttttg taatcttttt gtaatctttt tgtaatcttt tgtttctttt tagtatatat
     4561 ttgtttcttt ttgctccccc tggttacaaa ttagaaatat attctacttt tctctagttg
     4621 ttgcaatttc tttgatatta gcatcttctg gtttaatata agttggcact aattccctca
     4681 taataccatc tattttttca acctcttcat tgttagtaat ttctcttaaa agttttaagt
     4741 attttgttat tttttctcta tcaacatcta ttggtctacc aataaaaatc tttttatgct
     4801 ctgtatccaa cagaccttct tctgacataa gtagttcttc atacaacttt tctcctggtc
     4861 taagaccaga aaattcaatt tttatatcaa catctggctc aaatccactg aatttaatta
     4921 agttttttgc taaatccaat attcttacag gttctcccat atcaagtaca aatatttctc
     4981 cacctttagc catagcccca gcttgtatga ctaagcctac tgcttctggt attgtcataa
     5041 aatatctaat aatatctgga tgagtaactg tcactggtcc gccatcttca atttgcttct
     5101 taaaaagtgg aattacacta ccattactac caagcacatt tccaaatcta acagcaacaa
     5161 attcagtctg gctttcagca ttcatagtct gtataatcat ttcagcagct cttttagtag
     5221 ctcccattat gttagtaggg tttacagcct tatctgtaga tataaggaca aatttcttag
     5281 ctctatattt tgaagacaag ccagctatgt ttaaagttcc aaatatatta ttctttatag
     5341 cttcgccagg gctcgattcc ataagaggaa catgtttatg tgctgctgca tgaaatacca
     5401 cttctggttt atatttatta aatatttcat ccattctttt ttcttctctt attgatgcta
     5461 ttactgtttt taaatctaat ttatttttat attttctaag taattcttgt tgtattgagt
     5521 aagcattatt ttcataatta tcaactataa taagctcttt aggattaaat cctgcaatct
     5581 gtctgcaaag ctctgaacca attgagcctc ctcctccagt tattaagatt acttttccct
     5641 caatgtaatt acttatctct cttaaatttg tttttatagg ctctctaccc aacaagtctt
     5701 ctatctctac ttctctaatc tgttttatat caacttttcc atctataatt tcgtaaattc
     5761 ctggtattgt ctttatttta cagttagtgt ttttacatat atcaataata tctttttttc
     5821 tacgtttctc tatgtttgct attgaaaaga ttatctcatc tacattgtac ttttctacca
     5881 catcttttat atctgaagtt gttcctacaa ttttcacatt atggattatt cttccaattt
     5941 tatttttatc atcatcaatt atagccactg ctactttttt aagttctgga tttcttttta
     6001 gttcctgtat aaccatttct cctgcatctc ctgcaccaat tataaggata tttgaaacct
     6061 ttcctctaga atatgtcttg ttcatgacta ttcttatagc tctataggct attcttgcgc
     6121 cacttgtaaa cattataata aatattgtat ttaatacata aaacattata ggataatcaa
     6181 accctaataa tttatttatt atataaatcg gaattaaata agctaaagtt gccacaaaaa
     6241 tagatataat ttcttcttct cctgcatatc tccataatgt gttgtagcac ttaaacaatc
     6301 taaatgatac tatatgaagt actatatata tacacactga aattattata tcattagtgg
     6361 taaatgcatt taaattacct gtcaagctta aagacattcg aaacgctact agtacgctac
     6421 aaatatctag aattaatagt gttaaatgtc taaatttata ttttttaaaa agcctattaa
     6481 aaaagctttc tttctctaaa tctatacttt ctcgcataac tagaagccct cctttaaaca
     6541 gcaaaagcta cgcacctaag catagccaac aacatctatc ttttgctgta ttttttcatt
     6601 ttatttttta tatttatcaa atatagaatc ataaattttt tctatatcat taaagtttaa
     6661 attaacctca taattttctc taacaaattg acgccctttc ttacccatat taagtctaag
     6721 ttcatcattt tccattagtt ttattatagc attttttatt tcttctggtt gttttggttc
     6781 tacaattaag ccagtttcat tttcccatac agattctgga tgtcctccta catttgtaac
     6841 tataacaggt acttcacatg cttctgattc tatagctgcc actccaaatc cctctcttaa
     6901 agaaggaaat acagttacat caaaagaatt aaaagtcttg cttacatttt ctggagatat
     6961 tcttcctaaa aactgaacat cattttctat acccaattct ttagctaaat ttattaggtt
     7021 gtccatctga cttccagagc cacctatttt aagaattatt ttcttatctt tatattcatc
     7081 tttaagcatc ttaaatgctt gtattaaata ttctattcca tatttttttt ctaaactctt
     7141 tataattcct attacaaaag cgtcttcttt ttctacttcc ataggcttaa acctatccat
     7201 atctactcca aaaaaggtta catctacatg tttagctgta tatttattag cctctttagc
     7261 catatctcta ctatttgaaa agatatagtc tgcttttcta aagttatgtt ttataatttg
     7321 tttttgtata aaaccattat tagggaaatc ataaatatca gtaccccaaa cagaaataat
     7381 atatggatga tacccaagta gactgcctat aaatccataa ctacttgcat actgtgcatg
     7441 caatatatct ggctttattt catttactag cttctttatc tgctttattg aacctaagta
     7501 tcccgttttt ttagaaatat tttcattttt tagttcttct acattagaac caaaattata
     7561 aatttttatt ccttccatgt gacctccatt taaagagatt acatgaatgt catatccttt
     7621 atttttaaaa aaactacacc attttaaagt atgaggattt gacccatcag ctaaataaca
     7681 aacagtcttc ataatattta ccttcctatc gaataatact caattcctaa ctcttccata
     7741 tctttaattt tataacaatt tcttccatca aggactaaag gatttttcat cttttcctta
     7801 tacttatgta tatcataatt taatacatct ttccattctg tcattataaa acatatctct
     7861 gcatcttcta tcgcatcatc tacagaatca gcataaagta tagaatcacc aaatattttc
     7921 ttagtattat ttacaccaat tgggtcatat acagtaactc ttgcccctct ttcgagcaaa
     7981 tattttatat ttggtataga tggtgcttct cttatatcat cagtatctgg cttaaaagtc
     8041 aaacctaaaa cagcaacctt catatcttga aagctctcta ctaacttaga tgcctttcta
     8101 actaatttaa gtttttgact ttcattaact tctattgcag cttttactgt ttttaattca
     8161 taaccattat gttcagataa ccagtgaaga gcttttgtgt cttttggaaa gcaagaacca
     8221 ccatatccta ttcccgcctt taaaaactta cttcctattc tatcatcata actcattcct
     8281 aaagctacat cttctatgtt agctcctgct atttcacaaa gatttgcaat atcattcata
     8341 aatgatattt taagagctaa gaagttattt gatgcgtatt ttatcatctc agcactattt
     8401 ctatttgtaa ctattatagg ttgattaaaa ggcttataga tttcctctaa tatttctcta
     8461 gcccattcac tttctacacc tataacgatt cttgaagcat gtagagtatc ttttactgct
     8521 gttccttgtg ataaaaactc tggattagat gcaagttcta cttttacatc attttttagg
     8581 tttctattta tataatcttc tatctcttca tttgtaccaa ttggaactgt agattttatt
     8641 actactagag tatcttttgt cacagtttca gctatttgct ttgcaactgc ctttatataa
     8701 tctaggttgg ctgagccatc acttctctca ggtgtaccta cagctattaa tattacatct
     8761 acatcttcat aagctttttt atagtctgta gtgtaatcta ttcttcctct gttgtaattt
     8821 ttaaccataa gttcttctag gccttcttca tatataggtg ctattccaag tttcattgat
     8881 ttaacctttt gttcatctac atcaacacag actacatttt cattgcctac ctctgctaga
     8941 caaacacctg taaccaaacc tacatatccc gtcccagcta tagctatttt cattttttta
     9001 cctccaagta tataaaactt atactctttt tttaatggca ttaaaaacat agaaacaaaa
     9061 tacatataga ctttttggta aacttaaatt ttcaacattt ctataagtat tccaagttcg
     9121 ttttaatgct tttatcttgt tacttgatat agaatttcca actaatctgt aattcactaa
     9181 atcttcttga atcccatatg catatttttc ctttttaagt aactgtaacc aggttgcagt
     9241 atcttgtcct cttcttacca aaggcatgct gaactctcct acaatctcct tatcaatgac
     9301 cactgtagaa catcctatta tagtatttct aagtaatcct tcatagtcaa ttttcttagg
     9361 agctcttgca accttattcg tttttgaccc atcttgtctc atatatctat aagatgtaaa
     9421 agaaaaacct acattttctt ttaacatata ttctatctga atctctaatt ttctactatc
     9481 ccataaatca tcactatcta caaatgcaat aaatcgacct tccgcattct taataccaac
     9541 atttctagat actgcagctc cagaattttt ttcaagcttt atatatttta ttctagcatc
     9601 ttcttttcga aaacttttta taatttcact tgaattgtcc ttagaacaat catccactaa
     9661 taaaagctgc caatttttat aagtttgatt ttggattgac tttatcgtct ctgataaaaa
     9721 ctcttctgaa ttataaacag gagtaattat cgatactaaa ggctcattca tatttttact
     9781 ctccttatat agcaactagc agtactatac caacaagttt acttagatac taattttctt
     9841 tgtccaatat atttgtaata tcctaatggg aataatgcta aatacaacaa tttatcagat
     9901 acttttctta attcaccaaa ataatgacta tcagacaaca atgagtatct ggctaaattt
     9961 atgtaatttt ttatccacat atatggatat ctacttaatg gatatgtatt ttgatttatt
    10021 aaatataaaa actctaatgc tctacctctt aatgcatttt tacttaatat attcttagtc
    10081 aaaccatcat ccaaataatc tcttattatc ataggtttat ttacacatat aactttatag
    10141 tatttagaca tttcattcca tactacactt tctggtaaaa atctcacttc ttttctttct
    10201 ggaaaaggaa attttataag ctcatcagtt acaaatacta tgcttttatc tcccttaacg
    10261 ccatacttaa aatataggtc tgcaaagtga catattttct tatcttcagg catttctgtc
    10321 cctatcaaat ctccacttgg atatacacaa agtccaacaa ctccactaaa tccttcttta
    10381 ttctctaaag aatcccactc atcattagca atttcaagag aatctttaat taaaccatca
    10441 tcactatcta caatgaaaaa tagctctcca tctgcatttt ttacacctag atttattgca
    10501 gtatgttttc ctccattttc tttgtatata tatcttatat ttaatttatt ttctgatata
    10561 aatttttcaa ctaactcttt agtactgtcg ctacttccat catcaacaat caaccattca
    10621 aaatcattga aatcataagt ttgagctttt aaatcattgt acaaattttc aagtaaatga
    10681 gctctgttat acgtcggagt aaatacagta aatttatatt tatacattaa ttaaatcttc
    10741 ctctctgtct aacttttttt gattccgttt attccataat taataaagta gtatatagct
    10801 ttgtataaag gtattttttc tatatttcta tatatattcc aagtccacat agcagctttt
    10861 attttattac cacttaggga attgctagat tttctatata atgctagaac ttcatttatt
    10921 ccatgagcaa tatgcccttt tttcaatatc gataaccata aaacataatc ttcatgtctt
    10981 acaccactca ttcttatttc aaaatccaat ttagacttat caatcacaac agtaagacat
    11041 cctaagatat ttccttttaa taatcttcta taatctacat taggtggaac ttttattact
    11101 ttgtttaatt ttttatcatt ttcatccatc agttcatatg aagtaaatga aattacatag
    11161 tcattttcta acataaaatt tacttgtttt tctaacttac tactattcca ttggtcatca
    11221 ctatctaaaa aagctataaa ttgtcctgtt gccttactta gtgctaaatt tctagcatta
    11281 gagacaccct tattagtctc agtctttata cattttattc tactatcctg ttgcatataa
    11341 gatttgacta tattaggact attatctgtt gagcaatcat caataattaa catttcccat
    11401 tcttgataag tttggtttaa tactgatttt atggttgctt caataaattt ttcagaatta
    11461 tacatgggag taattataga tactaaattc ttcttcatat taccttcctc atcatttata
    11521 aaatatttat ttaattttac ttaatacaca atcatatatc ctcttgcagt tcttattatc
    11581 attatatctg aaaaacttat ttctttcatt tttttggtta tccataacct caaaattatt
    11641 ttcaataata tcaataagtt tattcacggc ttcatcatta ttataagcca aagacccaaa
    11701 catttcactt tttaaatcta tatatgaatc aactttttcc atatattcat ccaaatcgaa
    11761 ttggtaaaac aagataggtc tattcatata caaaaagtct atggctacac ttgaataatc
    11821 agttatattt gcagctgatt ttctgatttc atttgctaga gttacccctt tatctaaaaa
    11881 cacaattcgt tttcctgtta tattttcttt tatatcatct ataaactcat gcataagatg
    11941 atgcatataa atttttacat ttatatcttt ctcatctaat attttattta atttgtcatc
    12001 atttaaaaaa ttcattatat ttttaaaata atctgtatcc gtaaattttc ctgaactaaa
    12061 ttttatccaa tctctccatg taggagtata taatatatct cgagtttgtg caacttcatt
    12121 tgttggtaaa ttgtcatatc ttgcatttcc aattatataa actgagtcat ctaacattcc
    12181 ccaatcattt accataacat ttctttcaaa ttcagaactt gctgtaacta tattatagct
    12241 tttatatagg ttatctataa tatttttgaa ctttttgttc ataggttttc tttttttaaa
    12301 acttatagtc ccatgattta aaaacacttt tatcgttttt ttatgaaaat aattaagaac
    12361 aggtacagca aaattgtatg gtgctatatc tgctgatggt gcatgtgaaa atactatagc
    12421 ttttgaattg taataataca gatagttttc tacacttcct cttattaatt ttttgcctgg
    12481 aattttatca aatactttac tgtctttatt tactacccaa taagtttcta catcattatg
    12541 ttcttttagc atatactcat aaaaaaactt tgaattatca ttgtatatat cccctgaatg
    12601 tccacctata agccagatat ctctaccatt aaacttatgc ttattaaatg gatatataaa
    12661 aaaagccaaa agcatattta ttaaaaattt aaccttgtcc ataaacacca tcctcattat
    12721 aacttgtact gtctaaatcc attaattctt ccttttatag tggcactatg ataatctcta
    12781 ttggaactca atataagacc tcctatattt cttacaagtt ttgataccat aacttttgca
    12841 aacttataat tttttctata gactatttcc tttttgcaaa gcgctccaaa gcctcttcca
    12901 tagttataat ctttttgata cttttcttta gatttagaat gtttttttgc aggatggtaa
    12961 attatatcat ttccaaagta ttttccttta aaacctaaac tcaataagtt taaaacataa
    13021 tctatctctt caccagcacc aaactctcca ccaacaccca atttctcatc aaatcttgta
    13081 tagtctttac cttcaaaatc aataaaaaaa gttatggatg ttatagtatc caaaacgttg
    13141 gaacttgtga tatcacaagt tccatcatac attttcttaa atgcatcaac tttatttgaa
    13201 tccatagttt tgcaactgta tattttataa tctttatttt tattaaagaa atttataact
    13261 ttttctaatg tatcattttc gtagacacaa tcatcatcag ggaaacctat aatttgccct
    13321 acagcattgt ctatgcctac atttctgtta taactcaatc cttttttaga gctttttata
    13381 tactttatat ctaacttatc aatgtattta tcaactattt ctttaacttt gctattatca
    13441 ttttggtcta ctactataag ttcaaagttt ttgtaagttt gattttctaa gctgtccata
    13501 agtaagtata tatcatcata tctatttaat gttggcatta ttaatgatat taacattact
    13561 acctcctact tttcatcttc cacaaatcta tttccctttt atcatttaat atcacttctt
    13621 ttattagtaa tactactgca aaactaaatg aagcttctaa taataaattt tctgcaaaag
    13681 agtatagtaa aaatataaat aaagctacta aatatgcttt tttatctttt tctataaagc
    13741 tttctattcc tttatacatc aaatacataa ataatataca agcaactact cctagtacac
    13801 taattatata tacataagaa ctatctaatg gattagttgc ttttatagca ggactatatg
    13861 aatttccaaa aggttttaga aagttcccct tttcagccaa gtatacatgc cagaacttag
    13921 gtctacttgc taataatcta tttaataaag tgcttttatc aaatacttta ccaataatta
    13981 cactaaataa agtaagtatt atcggtaatg tctttaggag cattggtagt ccttttattt
    14041 tttttatatc tatccatctt attatttcta caaatattaa tcctgctaat atagtgaaaa
    14101 atccagttct actatatgtg gtttgataaa caaaaaatgc agaaccaaat aaaattattc
    14161 tatcccattt attataatct ttatatctta agaatatata tgctgcatat atagtaacca
    14221 tatgataaaa tacactattt ggattaccaa gtccaaaatc cattcttgta acaagcatat
    14281 ctattttttc tatatatcta tagtggactt cctgattaaa tattaatgaa ggaaatagat
    14341 taaataataa tatagctacc attaaaaaac atgccgatat tattaaataa tttttaacta
    14401 taaatttatc atctatttgc ttagctgata cagctaataa aacgattaat aatatagctc
    14461 cttccctatt taataaataa agtaagacac aaactccaac tagtaccatt tctgttatac
    14521 tttttctctt atcaaataaa aaaagtagca ttagtattcc tattgaagaa taaaataacg
    14581 ctgatttaat catccctata tccatatttg ggtcaataaa tgatatcata tactttctaa
    14641 gtaatatgat aaacaaaaca aatatataaa gatattttct aatcataata tttctatcat
    14701 catttaaagt cttcatttta ctttctccct tttagcttcg taaagtatta cttgcaaata
    14761 ttttacaaca cttccatatc ttatttacac ctgttaagtg tttgctttct aagaaataat
    14821 ttctcttttt agcccaactt tttccccacc aatgtatccc aaaagtgttt tctgttatac
    14881 attctggtgt aaagtcttct ttaaatccaa aaggataaaa atattctttg tcgtaaacac
    14941 gtattccttc ttcataatta tttaggtcaa tcttcttata attcttttcc ataagatgag
    15001 tcattatctt tggtattgta aacaatgaag aagtaagtac ttctttttca tagtactcca
    15061 ataagtcttt taaaaaagga tgtccttttt ctacacctat tatagcacca tttatatact
    15121 ctctatcatc ttcataccca cagattagcc tattattttc taaaagagga gttatatcct
    15181 ttaatatttg catatctgta tccatataaa ctccaccttg ttcatagagt acttttattc
    15241 ttgtataatc agatatgaat gcccaaagtt ttcttttata acattcttcc aaaaacttat
    15301 ttccttttat ttctttctct atatcaaagt ttttttcatt ccactctact atctcatatt
    15361 ctggaagctt ttctttccaa gaatttatac atatattttc tatatttcct tttggaccac
    15421 caaaccatac ataatgtatt tttttaggaa tcaaatgaac acctcctaca tatattcttg
    15481 tctatcttct tcttttaact gctctactat ttttttgaca tcttgagctt tatcttttct
    15541 acaaaccata actgcatcat ttgtagatac tactatcaaa tcactaatac ctattgtcga
    15601 tattaatttg tcatctgaat atataattga atttttagta tctatagtaa tattttcacc
    15661 tcttttgata ttaccttcat catcagttgg atatattgca cctagagaat cccaagaacc
    15721 aacatcattc catccaaaat caccaggaac tactataaca tcattacttc tttccataat
    15781 accataatct atagagattg attgtatagt tgggtatatt tcttttattt tttccatttc
    15841 ttcttttgtt cctaagtatt tacttatatc ttctaatttt tcatatactt ttggcaagta
    15901 tctcttaaaa tcttctagta ttttagatac cttccatacg aacattccac tattccatac
    15961 atacttttta gagtttacat attctttagc aatttcatag tttggttttt ctacaaactc
    16021 cactacctca tatgcaacat cttctatagt attttctcta ttaaaattta tgtatccata
    16081 tcctgttgat ggaaatgtag gagttatccc aatagttact agtttatcat tattttctgc
    16141 tatatagata gctttttcca aaatagattt aaattctttt tcatctttta tataatggtc
    16201 agctggatat acacacataa caccatcacc atattttttc attatgttaa atgctgcaaa
    16261 gcctatagct gctgctgtat ttctggcaca tggctctggc aatatattac tatccaaaca
    16321 tttatctttt actatatcct ttaaagcttc caattgcttc tcattggtta ctataaataa
    16381 atcatctttt tttgctagag aatctattct gtttatagtt tcgtttatta aagcatcttc
    16441 tccacttaaa tttataagct gtttaggtac ttcttgtcta ctaagtggcc agaaacgagt
    16501 tcctcctcct ccagccatta tgacattgta tactttcatt gtttatctcc ccttagcttt
    16561 ttatatagaa tccactacat ttaatatata agatattaaa ttgttcaact taaattctac
    16621 atcttcttga ttgtctgaat ttgctccaaa gtaaaattta attttaggtt ctgtacctga
    16681 aggtcttata gctatccaag agccatcttc aagtaaaaat ttaagtacat ctgacttagg
    16741 taaatcatca acactatctt tatagtctaa aattttatct acttttacat cagccacatt
    16801 atctatttta acacttctaa agtaaagcat catctctttt atttttttta ttccatctat
    16861 gccttttaaa gttactgact ttaagtcctc tttgtagtat ccaaatttat tatatgtatc
    16921 tatcaaagct tcatacaagt tcataccttt tgaagaatag tatgctgcca tttcacatat
    16981 cataagtgaa gatacaacac catctttatc tctagcatga gttccaatta agtaaccata
    17041 actttcctca tatcccatta taaaacttct atcattgctt tcttcaaacg cttttatttt
    17101 ttctcctata aatttaaatc ctgttaaagt atttaaacaa tctacattat aagcctttgc
    17161 tatttttgca cctagttctg atgtaactat agtttttatt attgtaggat tgtttttagg
    17221 aagtttattt tcttcaacta aactctctaa tatatatcta actaaaagtg aaccgacttg
    17281 atttccattt aagactacat attcaccact tgaatctttt acaacgatac ctactctatc
    17341 acaatcaggg tctgttccta ttatcaaatc agtgccatta ttctttgcca tttcaatagc
    17401 tatattaaac actgactttt cttcaggatt aggatacttt actgttgaaa aattagaatc
    17461 tggcaattct tgctccttaa ctaccattac attttcaaaa ccacattcat ttaagacttt
    17521 tcttactggc ttatttcctg taccatgaat aggtgtatat attattttta attttttccc
    17581 gtattcatct attatatttt gtcttaaaac ttgcttctta acagctttta taaactcatc
    17641 atctacttca ttatctaata tagttattaa atttttagat aaagcttctt taaaatctat
    17701 acttttaata gtactgtaat catcaatctt attaacttct gctattatat catttgccat
    17761 atctatacag atttgaccac catctgagtc atatacttta tatccattgt attctttagg
    17821 attatgactt gctgttatta ctattcccat agcacacttt aaatatctaa cagcaaaaga
    17881 aagttctggt gtaggcttta attcttcaaa aatataagcc tttacaccat aagcacttaa
    17941 agtctttgct acttctatac aaaactccct agacatgtat ctactatcat gtgctataac
    18001 tattcccttt tgcttacctt cttctccttg agttttcatg atataattta aaacgcccag
    18061 tgtcgcacgt ctaacagtat aaatattgat tctattagtt cccgctccta ttatacctct
    18121 taaaccacct gttccaaact ctaaattttt ataaaatcta tcttgtattt ctttttcatc
    18181 atcttttata cttaatagct catttttcgt ttgttcatca aaataaggag aatttaacca
    18241 catctcataa ttattcttat aatccatatt ttcatccttt ctgactctaa tttaaaattt
    18301 caaggtaatt atctatcttc taagtagttt ttgttttcct tttcttaaaa attcaaaagc
    18361 taaatccatc tcttctacct taaataaaac tatcaataca gtatatacag aagctccacc
    18421 taatactcca gctccaactg atataatttc tttaattgtt cctgaaccca taaatgcata
    18481 tagattatta tatatcaata aagtagctac tgccatcaca ccagatgcta ccaaactttt
    18541 tagaccagtc tttataattt tatcccctcc aaaatatcca tttttctttt taagagatat
    18601 aaataataat ataactgtaa ttatgtttga tgtactagtt gatatagcaa tacctgcatg
    18661 acccaatggt cttatcaata ttaaattaag aactatattt attattaaag ctatacttcc
    18721 atttatcatt ggcgtttttg tatctgaaag agagtaaaat gccctatata agacatctct
    18781 tactccacaa gccaataaac ctagtgaata taatctaagc gctatagatg taaggtctgt
    18841 tgataacgca tcaaatttac ccctttggaa taatattcta acaagaggct ctgctaatat
    18901 aatagctcct atagatatag gaagcaccac caatataata caatttgaag attttacaat
    18961 agtgcttata aatccttctt tattatcttt accagacatt tttgctagtt taggatatat
    19021 aactgttatt atagatgtaa caaataatgc cataacaaac tcatttagcc tatttgcata
    19081 gttaagagca gacaactttc catcacccaa agtggttgcc aataatcggt caacaaatat
    19141 atttaactgt ccaacagcta ctcctaccaa cataggcaat actaggttga ctaactcttt
    19201 tatggactca tcctttaaat ttatatatgg cttatacttg taggacttct taaacgcaaa
    19261 tggcagttga aacaatagct gactcgccat agccaataat gctcctgctg gcagtatcca
    19321 tacattttta cctgcactta gtgctattgc tgctattatt ataatattat atgggattcc
    19381 tataagccca ggaattacaa aactgtcatt cacctgtaaa aacgaactaa atatcttact
    19441 tccactcagg aataaaactc ctgaaatcat tattttagta aataatattg ctgcttggaa
    19501 cttagcaggg tcgtttctaa atcctgctgc aaatactgta acaaattgct ctgtaaataa
    19561 aagcccaata atagctatta ctatagccat tatatagcat atatttaaaa cattattagt
    19621 aaattttaag gcaccttcat ctccgagtct tttctttgtc tcaaagtaca taggtatata
    19681 agttgtagca actgcagagc ctatgactgc tatgattaaa ccaggtatat ttaatgttaa
    19741 tacaaatacg tctgcataca tacctgtccc atagaaattt gcaagtacaa gttctctaag
    19801 gaaacctaat acttttgaaa acatagtggc tgccataatc cacaatgctg ctttagctgt
    19861 ttttgacatc ttctttctcc ttatctaatt taaactctaa aagtagaact tttaaaactg
    19921 cttataaaca ttattctaat taagtaaatt taacctatga aaagcttcct tataaatcaa
    19981 aaactatttc gactcatatc taagtgccct aaatgtaatc aatagtaata atagtattaa
    20041 tactatacaa gataagaatg ttagattaaa tatgctctta aatagactat aatcacctac
    20101 tgtataccca acttcaacta catcatttat attgctatct ttcaagaatt tatcttgtgc
    20161 acgacttaag ttatcactac aaatcagtat tggcatattt tcttttgctg atactactcc
    20221 caaagaaata tattcgttta catcagatct actaccaaag tctaatctag cagtatagat
    20281 tttctttata tctttttcat cataaaactt ctttattaaa tcaacatttg tacttgactt
    20341 atttatttgc tttattcctt ctatattttt taagttagaa tcaatctctg caataaactc
    20401 ttttgaatca cctaatgcat atattttttt aaggtttttg ccttttaaga aatcaatttg
    20461 ttcctcaaac ccttcatctt tagcccatat tattggcatt ccacttttag cggcataaga
    20521 atatacagat atggcgttct ctaatcctgt tgttgagcta agtaaaaatg cttcatcaaa
    20581 tcctactgtt ctatacattt ctctagctat ctttaaagat gtatcatatc tgtctttccc
    20641 tcttattctt tctactttca ttcccatttt ttctatatta cgttcagcat ctttagatac
    20701 tgcctttaaa ccacctacta tcgtaacctt ctctggacct agttctttta gataatttct
    20761 tgttactcta ccaagatttt tactcttagt tacaactatt ggagcatttt ttgcatatgc
    20821 taaaggtgtt gcacttatac tatctacaat tgactcttca tttacaagaa ttacttcttt
    20881 agaatcctta aatgctcttt tatttaattc tgtcgaaatc tctggtatac tcccttctat
    20941 caactctctt ttgggatttg catctgcaaa tactcctatc gtactagaaa tagagattaa
    21001 caaaactaac gataatatag tagaaatttt tttaatcata agttctcatt ccttttcttt
    21061 tttgtatttt ttgacaataa aaaagctctt tttaaagagc tttttcattg attagtaatc
    21121 taatatttta aaattatcta gctccgtctc ctgtcaatac tactttaaca gttttaaaga
    21181 gtatcttaaa gtcaagtaat atactcctat tttttatata gtaaatatct tctttaagtt
    21241 tttcttcagg agtgatttcg tatccaccat ttacttgtgc ccaacctgta agcccaggtt
    21301 ttatagcgag cctgttgata aatccaggta tttcctcatt aaattgaaca gtaaagttcg
    21361 gtctctctgg tctaggacct attaagccca tgtccccctt taatatatta aacagctgtg
    21421 gaatctcatc aattctagtt ttcctaataa actttccaac cttagttatt ctaggatcat
    21481 ctttttcagc ccattgtgcg ccaaattttt ctgcgtctgt tctcatagat ctcaatttat
    21541 atatatagaa tcttctcccg cattttccta gtctttcttg cttataagtt attggtcctt
    21601 tgtcctctat tttaataaga attccaaata ttgctattaa aggtaaacct atcacaagtc
    21661 ctattataga taaaactaaa tccataattc tttggtacaa gtcgaataaa actgttcctt
    21721 ttactatgct atagtcaatc tcacttgcaa tttctttatc tgcccctgga tagcttttaa
    21781 ttttaggtat ttcacactca tcattagtat atccagacat agttacctcc atattggaat
    21841 aaaaattttt ttaattctta atttcaactt atttctatct tatttttgac ttatttctat
    21901 attatactca ataatgagca gattatcaat acaagaaaca tcattgtaaa caatttgatt
    21961 ctttacaaaa atatatcatc tcttgttata ttttacatta taacaggaga tgatatgttt
    22021 tgctttaata ttttctattt tattttactg tatcttctat tcctattcca atttgagttc
    22081 ccattgtttt tactttttct gcagtattta acttatccat atcgctctta ttgtcaataa
    22141 atgcacattc aacaagtatt gctggataat tgttatttct taacacatat aggtaatctt
    22201 ttccattatc aagtgttctt gtcttgattc ctctattttt catgttaaat ttttctagta
    22261 ttctttttaa gatatttgat gctgcagtct tagttgttcc accattttta tcttttactt
    22321 tgtaataaat ttccacacca ttaccagctc cattagacgc attgtaatgt atacttgtaa
    22381 ataaatctgg ctttatagta tttgataatg ctgttctttc tcctaaagcc attgttttat
    22441 ctgtatctct agtcataaca acatttatac cttttgaacg taaatattca gtagttgcta
    22501 atgctgtatt aagagtatat ttcttttctt gtgctccacc atttaatcca cttgttgccc
    22561 ctgaatcaga acccccatgg cctgggtcta tcactacagt ttttcctgct gctgaacctg
    22621 aattatctgg ttctgttggt gcattgtgtt ttgataaact agatgctata gaatttataa
    22681 cactatcttt cattccatca ccaatttgat atactgtttc tgctgttttt tctgataagt
    22741 ttgtactatg gtatgcagaa acataagttt ttggagttat aagtattgga gcttttaatt
    22801 tagctgctaa aggtcctgct gttattgaat caactattat gtcagatttt gcaactaaca
    22861 ttgcactcaa ctctttatct ggatagaaag tcttaattac atttgcattt gtttcatgtc
    22921 tatctgctcc agaaactcta ttttttgaag taccattttt tgctatttta gatatttggt
    22981 ctataatttt tgatgataaa ctttgactac cacctatgta ataagcttct tctaaacctt
    23041 gtgaagataa ccaattatat gtaccttgtg gtacagaatc tttattagct aatattatag
    23101 gttgttggtc ttcaccagct tttgatgaaa tattaagagc atcatattca ccagcatatc
    23161 catttgctat gtatatttta ttaacatcat gatacttatc tatttctttt gctataagta
    23221 aagatgtttc atgtctatca actccaccaa tacgagtcac atttacattt acagcagatt
    23281 taatttgaga cactgccttt tgtgaaactg aaccatcatc acctataatt attacatcac
    23341 ttggatttag acgttttaac tctgcttttg tactttctgg tatatcatct ttatttgcaa
    23401 gtaatattgg tgcgtcatat gtagatgcaa gtggagtagc tgtaattcca tctgctgcta
    23461 attctccatt aactattaca accttactag acccatcttt ccaaccttgt ttacttatct
    23521 caacagcagt ttcaaatcta gtagctccag caagtttttt tactggtgca gaagtttttc
    23581 tcatatattc atttactgct tttgttgaat ttgcatcact tattataaac tcttgcgtaa
    23641 catcataatc cttaagattt aatcccatag actctaagtc ttctttgttt aaaactttct
    23701 gttcatcttt accatttatt tttatgcttt gaagttccca tacatttaag tattctgggt
    23761 ctttgttata gtttattaca ccttcatatg aatctgtctt ttcattgtaa tttaaagtag
    23821 cttcaagtgg catatcataa cacataaaat ttaatgttat tgaatcagca tttaattttt
    23881 ctttaaattt tacacttgca ttagatttac ttcctaacac tacacttttt ctttgtaaat
    23941 ttatattaat atctgaatta gattctgtat ttgcagtagt attttcagcc gctaaagcta
    24001 cgtttggtgc caccattgct acagacaaca ttcctgttgc caataatttt gttgtttttt
    24061 tcatcatatc ccccctaatt ttttggttct ttaaatcttt aaatcttttt taatacatct
    24121 taagcttgtt ttatattaca taaattcaat attatgtaaa tcttttttca ttatagcaca
    24181 aaaaattaca aattgtaaac tttttgtaat cttttatggc atggttttta aattttatcg
    24241 aggagaaatt ttgattatat ctgatagttt ccgtatattt gaacaaatca aagactttat
    24301 catctaaaaa tattgcacta aattttaata aatatttttt tcttgtacat tatagacatt
    24361 actataaccc agaataggac atacaataaa gaaaatataa gagaatccca agttgtacct
    24421 gcccaaggtg ttataagttc atatgtagtc cactctacca aggtagttgg ataatcgact
    24481 ttgttcgtaa gttttggaac attccaaaaa acatgactta atatttcaag gcacatatat
    24541 gtaaaaatag ggctacttcc aagtgctatt attggggtaa atattttact tttatttttg
    24601 atgtcgcata taaaataaaa tattgacagt aatataccat aactgccagc cattaaaagt
    24661 acaaatgagc tagaccataa tcttttatta aatggaaaat actgattaaa gataaaagca
    24721 cctattagca ggattatgct cataaccagt attttaaaga attttttata ttctcctaga
    24781 ttttctttta aaagtacaca tcccatagtg catcccaaca tacctgaaga aattgctact
    24841 atactagtta aaaaaccatc tggttcaaat tctggcttgt acaaatgtcc cttgaagaaa
    24901 tgtaaatcca ctaactgtgc aagagaacct tctagctcaa atccataagg ttttgctaat
    24961 atgtagtacc caactattat aaatgtagat atacatagaa aagtaattat tgtagcagta
    25021 cttccaacat ttaatttttt tagtagtaaa tatactaaac tagtcacaaa atagactaat
    25081 cccatacgtt gaagtactcc aagtattctt acagtatcta aatcaggatt tcctaggtaa
    25141 tttaagaaaa atccaaataa tatcaataag atacttctct tgaatatact aagtattata
    25201 ctcagtgtgg atttattatt ttttatctta ctatttattg atattggaat tgtaacacct
    25261 aaagatataa caaaaaaagg aaatgcaaaa tctgcaagtg ttacaccatg ccatacagca
    25321 tgtcttaatt gaggatacat tctcatccaa gtcccaggat tattgcagac tatcatgagg
    25381 gctattgata gccctcttat tatatctatt gatttaatcc tactattcat aagtttagaa
    25441 tctacagcat tatttgaaat t
//
LOCUS       DAKBNN010000034        25292 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000034 DAKBNN010000000
VERSION     DAKBNN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.034"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            121..405
                     /locus_tag="OUB02_001240"
     CDS             121..405
                     /locus_tag="OUB02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCT8885011.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            518..1438
                     /locus_tag="OUB02_001241"
     CDS             518..1438
                     /locus_tag="OUB02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HCT8885012.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            1528..3900
                     /gene="spoIIE"
                     /locus_tag="OUB02_001242"
     CDS             1528..3900
                     /gene="spoIIE"
                     /locus_tag="OUB02_001242"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HCT8885013.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            4251..6143
                     /gene="pepF"
                     /locus_tag="OUB02_001243"
     CDS             4251..6143
                     /gene="pepF"
                     /locus_tag="OUB02_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCT8885014.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            6257..7222
                     /gene="galU"
                     /locus_tag="OUB02_001244"
     CDS             6257..7222
                     /gene="galU"
                     /locus_tag="OUB02_001244"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCT8885015.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNESRELEIQK"
     gene            7722..9326
                     /gene="rho"
                     /locus_tag="OUB02_001245"
     CDS             7722..9326
                     /gene="rho"
                     /locus_tag="OUB02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCT8885016.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            9479..9679
                     /gene="rpmE"
                     /locus_tag="OUB02_001246"
     CDS             9479..9679
                     /gene="rpmE"
                     /locus_tag="OUB02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCT8885017.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            9824..10717
                     /locus_tag="OUB02_001247"
     CDS             9824..10717
                     /locus_tag="OUB02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HCT8885018.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRVVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            10735..11583
                     /gene="prmC"
                     /locus_tag="OUB02_001248"
     CDS             10735..11583
                     /gene="prmC"
                     /locus_tag="OUB02_001248"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCT8885019.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            11627..12691
                     /gene="prfA"
                     /locus_tag="OUB02_001249"
     CDS             11627..12691
                     /gene="prfA"
                     /locus_tag="OUB02_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCT8885020.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            12820..13500
                     /locus_tag="OUB02_001250"
     CDS             12820..13500
                     /locus_tag="OUB02_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCT8885021.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            13529..14569
                     /locus_tag="OUB02_001251"
     CDS             13529..14569
                     /locus_tag="OUB02_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCT8885022.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            14586..15035
                     /locus_tag="OUB02_001252"
     CDS             14586..15035
                     /locus_tag="OUB02_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCT8885023.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            15052..15504
                     /gene="rpiB"
                     /locus_tag="OUB02_001253"
     CDS             15052..15504
                     /gene="rpiB"
                     /locus_tag="OUB02_001253"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HCT8885024.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            15540..16169
                     /gene="upp"
                     /locus_tag="OUB02_001254"
     CDS             15540..16169
                     /gene="upp"
                     /locus_tag="OUB02_001254"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCT8885025.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            16470..17285
                     /locus_tag="OUB02_001255"
     CDS             16470..17285
                     /locus_tag="OUB02_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCT8885026.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPFTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            17511..17948
                     /locus_tag="OUB02_001256"
     CDS             17511..17948
                     /locus_tag="OUB02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HCT8885027.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            18133..18360
                     /locus_tag="OUB02_001257"
     CDS             18133..18360
                     /locus_tag="OUB02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HCT8885028.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            18365..18751
                     /locus_tag="OUB02_001258"
     CDS             18365..18751
                     /locus_tag="OUB02_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCT8885029.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            18754..19458
                     /gene="atpB"
                     /locus_tag="OUB02_001259"
     CDS             18754..19458
                     /gene="atpB"
                     /locus_tag="OUB02_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCT8885030.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            19527..19787
                     /gene="atpE"
                     /locus_tag="OUB02_001260"
     CDS             19527..19787
                     /gene="atpE"
                     /locus_tag="OUB02_001260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCT8885031.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            19910..20407
                     /gene="atpF"
                     /locus_tag="OUB02_001261"
     CDS             19910..20407
                     /gene="atpF"
                     /locus_tag="OUB02_001261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCT8885032.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            20404..20949
                     /locus_tag="OUB02_001262"
     CDS             20404..20949
                     /locus_tag="OUB02_001262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCT8885033.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            20966..22468
                     /locus_tag="OUB02_001263"
     CDS             20966..22468
                     /locus_tag="OUB02_001263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCT8885034.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            22496..23374
                     /gene="atpG"
                     /locus_tag="OUB02_001264"
     CDS             22496..23374
                     /gene="atpG"
                     /locus_tag="OUB02_001264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HCT8885035.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            23387..24781
                     /gene="atpD"
                     /locus_tag="OUB02_001265"
     CDS             23387..24781
                     /gene="atpD"
                     /locus_tag="OUB02_001265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCT8885036.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            24784..25044
                     /gene="atpC"
                     /locus_tag="OUB02_001266"
     CDS             24784..25044
                     /gene="atpC"
                     /locus_tag="OUB02_001266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HCT8885037.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        10194 a         2730 c         4818 g         7550 t
ORIGIN      
        1 aaacatattt atagcaacaa gaaaaagtat aaaaagaaaa tcaaataaaa aattaaagaa
       61 caagaaaaaa tcaaaaccta agaagaaaaa aaataaaact aaaaaaaggg tgtaggaaaa
      121 atgaacttaa gaaaaaaatt ttcaggacag attattgtta tcagtttatt tttaggaata
      181 tcaattttta gtatgatgac aggatttgta tttgagtata caaaagctaa ggaatataaa
      241 aaagaaatag caagtctaaa taaacaatta aaaaagactg aaatacaaat aaacagctta
      301 aagaaagatg aaaaatcata tgaaggagac ttagaggata tagcaagaaa aagattgaat
      361 atggtaaaac caaatgaaac agtttatgta gacataaatc gttgattaaa acttgctaat
      421 aagtttacag atgtattgat tttaaaattt aagatttaat aaaaaagtca ttcagttaag
      481 gatggctttt tgtttttatt gaggagggaa tttcataatg aaagtaggag ctatagatat
      541 tgggacaaac tcaatgagat tattgatagc agattataga gatgggaatt ttataaacag
      601 aaaaaaattt gttaatacaa ctagaattgg ttatggtgtg gatagtaaag gatatataaa
      661 agaagatgct atcaaaagaa atataaaagc attaaaagaa ttttctgata agtgtaatga
      721 tgaaaagtgt gaaaaaattt attgtatggg gacatctgct cttagagaca gtaaaaatgg
      781 agattacttt gttaaattag ctaaagaaga aactggaata gatgttgata tagttacggg
      841 aatagaagaa tcaaatttgg gctttaaggg tgttttagaa ggaatacaag gtgatgatac
      901 tattattgtt atagatattg gaggaggttc aacagaattt attgtaggtg attcagaagg
      961 aattatattt aataaaagtg agaatgtagg tgctcttagg atgacagaga ggtttttgaa
     1021 gcacgatatt attgaagatg aagagtttga agctatgagt tcatttatat atggtgagat
     1081 tgataaaacg ttaaatcata taaaagacag aggattaaaa actcttatag gtataggagg
     1141 tacgattaca tcattatcag caatgaatca agaattagag atttactcta tggaaaaaat
     1201 acataatagt aaaatacaca aaaaagaaat agaagaaatt ctacaaaaat taataaagat
     1261 gacattaagt gataaaaaga acttaaaagg gctacaagaa aaacgtgcgg atattataac
     1321 tgctggtgta aaaatattga atataataat ggaaaaatta gaaatagaag aaataaccgt
     1381 aagtgaatac gataatttgg aaggcttaat atgtcaaaag tcaaaaaaaa tgtattaata
     1441 ttttttgatt tttaaaaccg attatgacaa aaaaaattac tcctctttgt tacaattctt
     1501 aatataaaca aaagaggagg tttttttatg caaagaagtg tagcagttgt gaagaataag
     1561 actgtaaaac caaatgtcat aaaattaata agcggatata ttgattttac tacggcggca
     1621 ttcgctgtga tgggattttt acttagtagg tctattatag tagactctgt agctccttta
     1681 ggaattgcct tttttatatg tgcatcaaag attgataagt acaagatacc tgtattcata
     1741 tcaacattat taggaatatt gttatcatct aatgattttg gaaatgtagc aaaatatgct
     1801 gtgtgcctta caatatttat ggctatgaat aaaaaattaa aattatcaga ttcaattgct
     1861 aaggttgcat ttataggtgc aataatacta cttccaatct ccatgggaca agctttcttt
     1921 tcaaataggt atgtatatgg tcttcttata gcaggagtag aagcaataat aatgtttata
     1981 tctatatatg tgttttctta tgggataggt ttaatggcca atgtcaacaa taggatgtct
     2041 gtacggacag aagaagccat atcaataagt ttactgctaa cctttagcat aatgggaata
     2101 ggagatatat ctctgtttgg aatatcaata aggaccattt tatcgaccgt attgatattg
     2161 ctgacctcaa tagtaggtgg agctaccatg ggagcatcta gtggtgtaat tattggtgta
     2221 gcatccattt taaataatat gacatcagct gtttatatgg ggatatattc tttttcagga
     2281 ttaataagtg gagcatttaa taaaataaat aaatatttct gtatactagg atatattcta
     2341 agttggacaa taatatacct atatacttct ggtattactt ctaatatgat gcaattaaga
     2401 gatatactat taggttgttt gatagtacta gtattgccag agcgattatt taataagatt
     2461 gaaaaattaa taaaaagtaa tgtggcttca aatgaaatcg tatacgatta tattatgaga
     2521 agcaaaaacc taactaatag cagattgaac agtatttata aaacttatga tgaccttgca
     2581 gatacatttg ataagataag agaaaaagac aaagtgctag accaaagaga catagctaat
     2641 gtaatagata tgattcataa tgatgaatgt aaaagttgta gtatgagaag aatgtgctgg
     2701 gagtcaagat ttaatcatac atacactatg atatataaca ttcttgaaaa aattgaggag
     2761 aaaggggagt taagtctaaa cgatatacct aaaaatttta gaaaggaatg tatgaaacca
     2821 gagtcaatag tgaaaatatc aaatcactat tacaaaatgt ttgtattaga ttatgattgg
     2881 agtgtgaagt tttcagaaag tagaaagtta atagcaaatc agataagaag tatttcaaaa
     2941 tccattaaat ccttatctca agatttagaa ggcgacataa tgctagatat agaaaaggaa
     3001 aaaaatatat atgaacagtt agaaagatat gatataacag ttgataaagt aagttactta
     3061 acaaaaagta acagtgagtt tgaaatatca atagaaaaga aaacttgcca tgatggatgt
     3121 atgtgtgaag ataaaattgt aaatataatt tcagacctag taggtgaaaa tatgtctgtt
     3181 agaaaaatag gatgtcactg tttaggaggt aaatgcaagg caacattcgt taaatctcaa
     3241 aaatataagg ctgttacaga agtttcagca atgtcaaggg atgggcatat cctttgtgga
     3301 gacaattaca catacatgga gataaatgat ggtaaataca tgatggctat aagtgatggt
     3361 atgggaaagg gtaaaaaagc atatgaagaa tcttctgcca ctattgatat actggaaaaa
     3421 atgatagatg caaaaattaa agatgagata gtcattgata ctataaacaa tatgttgttg
     3481 ttaaaatctt cagaagagat gttttcaact ctggatttgg gaattttaga tttaaaacga
     3541 ggttgtttag aaactataaa gatgggagcg tgttctactt atataaaaag agaggatgga
     3601 gaagttgact taatatcatc atcatcatta cctgtgggta tattatcaga tgtaaaaatt
     3661 gatagaaaaa atgtaaaagt taaggaagga gactatgtta taatggtttc tgatggaatt
     3721 gtagatgcag gaagaaataa caatttagga gacaattggt taatatactt cttaaagaat
     3781 atagagacta caaatccaaa ggaaatttca aatttaatat tggatagagc attagagtta
     3841 caagcgttac aaatcgaaga tgacatgact gttttagtta cgaaaatatg tacaaaataa
     3901 ggtttacaag attatgtgta taagatgtgg ataacttgtg tataatatgt taatagactt
     3961 ttgaaaaaag tatagttatt aacaaaaata acatataaat caacaaaatt tgttgataag
     4021 tatggcagaa atgttaataa aatgtaaaaa aagaaaaaac aaaagctatt atcaaaagag
     4081 attcatgttg aaataactaa atttctaagt aaagaaaacc tgtgaatatg ttgattatta
     4141 tgtggataac tattaattcg atatttattt atatagtatg aataattgaa taatatatct
     4201 aaaaaaagcc atcctattat taagtaaact tattgaaagg atggcttttt atgaatagaa
     4261 aattgtgcaa agcaataata cctttattta tttctatttt ttgcatgtta ataataagta
     4321 taaaagctga ccaaggatat caaaagaata atgacagagc aattactgtc atgaataata
     4381 atgatactga agatgatgct gaaaaaaaac aagaaataga ctatgaaaaa acatgttgga
     4441 atttgcaatc tttgtttaaa agtgatgacc aatggaaaaa agaacttaag agttttgata
     4501 aagatactaa agagttgaaa aattacatag gtaaagtaac aaaatcgcca acacatttgt
     4561 cttttgcctt gaacattaaa gaaaaacttg atataagact aaataaactt tctgcctatg
     4621 taaaattaaa acaagatact aataaaaatt cgtataagta cttagatatg aatgatagta
     4681 tgagtaaatc ctatgggaat tatatgggga tttgttcaga tttagagtta gaaatactaa
     4741 aattatcaaa caaagattac aagaaaatta tttcaaataa aaatataaat aggaaatatg
     4801 gtgcatattt aagagatata agaaggaata aagtacatta tttggatgat aaatcagaag
     4861 atattctaag caaagtatct tcaataagta gtcttccaag ccaagtatat gaattattta
     4921 gaaatatgga tagaaaaact aatttgacac cagctcaata tgcgagtgaa ttagagtcat
     4981 cagatagaga aaatagaaaa aaagcttatc aagatgaact tataacttac aatgataata
     5041 taaatacaat tgcaggttta attactggtc aagttaaaaa gaatattttt tattctcagg
     5101 aaagaggtta tgagtcttct ttagaaatgt acctggaatc tgataatgta gatgaaaaag
     5161 tatacaataa tttgataagg actgtaaata gtaatatgga cagtctacac aaatatatag
     5221 atttaagaaa aaaagtatta aaattagata aggttcattc atatgatatg tttgtgccta
     5281 tggttaaggc tgttgacaat aatattaatt atgaaaaggc acaaagtata gtatattcag
     5341 cgttgtcacc tttaggaaaa gaatacaatg atgtagtata taaagctttc aatgaaaaat
     5401 ggatagatgt ttattcacat gaaaataaag taagtggtgg atactgctta tctgtttatg
     5461 aaaatcatcc atatatactt ttaaattata ataattcatt aggttcagtt tcgaccttat
     5521 cacatgagct tggacatgct gtatatgaat atttaagttc taagaatcag aattatttta
     5581 attcaaatcc gtctatattt actcacgaag ttgcatctac aacgaatgaa gcattacttt
     5641 acgagatgct tataaaagaa gctaaaggca atgatgaaaa agcttattat ataacacagt
     5701 atttagactt gataaaagat actctttata cacaaactat gtatgctgaa tttgaaaaaa
     5761 ctatacacga gttagtagaa aaaggaaaaa gtgtaaacgc attagtatta aatgatgttt
     5821 ggggacaact tcttaaaaaa tattatggac aaagctatga attagaccaa ttatctaagg
     5881 tcggatgggc tagaatccca catttttata atagtttcta tgtctataaa tatgctacag
     5941 gttgtagtgc aggagtaagt tttgcccaag acatcctaaa gaatggttca gataactata
     6001 tgagctttct taaaaaggga agttcagatt atccaataaa cttgttaaaa tcaagtggca
     6061 taaatttaac taatacaaaa cccatagaaa atacaatcaa aaaatttgat agtttagtag
     6121 cagaacttga aaagctatta taaacattat tgtaatccac atggtaaata tatcgaatta
     6181 atgataaaat atactaaaag tttaaagtcc tatcaagata ggacttttta tttattaatt
     6241 agtgggagat gattctatgc aagttaaagt taaaaaggct gtaataccag cagcaggttt
     6301 aggaactaga tttttgccag ctacaaaggc acaaccaaaa gagatgttac caatagttga
     6361 taagccaact ctacaataca taatagaaga agctgtggcc tcaggaatag aggaaatact
     6421 aataataaca ggtagaaata aaaaatctat tgaagaccac tttgataaat ctgtagaatt
     6481 ggaacttgat ttagagaaaa aaggtaagaa agaattgttg gaggtagtac aaaacatatc
     6541 taacatgatt aacatacatt atataagaca aaaagagcca aaagggttgg gagatgcaat
     6601 ttattgtgcg aggcatttta taggtgatga gccgtttgca gtaatgttag gggatgatat
     6661 tgtagataat gatgtacctt gtctaaaaca actaacagat gcatatgagg aatatagaac
     6721 tacaatattg ggtgttcaaa aggtaaatca agaagatact aataaatatg ggataataga
     6781 agcaaaaaat atagaaggta gagtttacaa ggtaaaagac atggtagaaa agccagagtc
     6841 aggaaaagct ccttcaaata ttgctatttt aggtagatat ataataactc ctgaaatctt
     6901 tgatattctt aaggatttac caccaggtaa aggtggagag gtacaactta cagatgcttt
     6961 aaaaatatta tctaagaaag aagctatgta tgcatacaat tttgaaggca agagatatga
     7021 tgttggggac aagctaggat ttttagaagc tactgttgac tttgctctaa agaaagaaga
     7081 ccttaaggaa gattttataa aatatttgaa acatgtatgt agtgaatttg ataaaaataa
     7141 caatgtgtta tacaataata cagatgaaaa aatagattta attgaagtca atgaatctag
     7201 agaacttgaa attcaaaaat aattaatgaa aggtgatgta tggtggcaaa atcaagtcag
     7261 aaaagaatta atagaataat agctgttggg attgtgggaa tcctcgtaat aacgagtata
     7321 acagctgttt tgagtatgat tgcaactatg taataagata aatgacagca gttgatatac
     7381 ataaatataa aaaccatata taacttgatg tagtcaaaaa cttacaatat tttagttata
     7441 tatggttttt agaatttata aatgtaaaaa aaaattttta taatagttgc atattttaaa
     7501 ggaaaatatt attataagat ataaatatag tgataaattt ataaaaacgt gctaagattg
     7561 gacttgaaat acagtaatat agtaggtaat aatgttttaa attttattta ttgaccataa
     7621 tatataaata atgatattat tagacttaac tgtttaaata aaacttttct ttttcatgcc
     7681 ttcaatttcc aaaataagta tgtatataaa tgaatatata gatgaggagg atttttttga
     7741 ttttagatga taaagtatta gaaggattag gtagtaagac cttagttgag ctacgagaga
     7801 tagctaaaga attaaaaata aaatcaatta ctacatataa gaaaaatgag ttaattgaga
     7861 ttataaattc caaaagtaaa aatgaggaaa aagtaaacga agagactaaa gaaaaaaatg
     7921 aagatattaa agaagacata aagaatagaa aagaaaatat agagcgtgaa agtatagaaa
     7981 aagaagttga atataagagc cctaccaaat catacaacaa gaaagaaata gatacccaaa
     8041 atatttccca aaaccaagaa catagaaatc aaagaaatga ctataataag acaacgagtg
     8101 taaatgatag caataagtca tcaaatatat ctaatagaat ggtcagaaat aataataaaa
     8161 actattatat gcctaaacaa gttgatgaat ctaagatagt agatgaattt aacacatcaa
     8221 aagaggatga agtagtaggt gtacttgaaa ttctgccaga tggatttggt tttttaagag
     8281 gttcaaatta tttgtctact gaaggagatg tttacgtttc accatctcaa ataagaagat
     8341 ttaatatgaa aacaggagac aagattaagg gaataactag acatccaaaa agtggagaga
     8401 aatttagagc acttttatat gttcaaaaaa taaatgatga aaaccctgac acagctatac
     8461 aaagaaatgc gtttgagaca ttaacaccaa tatttccaga agaacgactt acactggaaa
     8521 ctaatagaaa tgaaatagct acaagaatta tagatttaat ttcacctata ggaaaaggac
     8581 aaagaggatt gatagtagca cctccaaaag ctgggaaaac agttctttta aaaagcgttg
     8641 caaatagtat agctaaaaat catccaaatg ttgaacttat agtactcctt atagatgaaa
     8701 gaccagagga agttactgat atgaaagaat ctattgaagg agacgttata tattccactt
     8761 ttgaccaagt atcaagtcat catgtcaagg ttgcagagat ggtgttaaat agagctcaaa
     8821 gattagttga acatggtaaa gatgtagtaa ttttactaga tagtataact agattggcaa
     8881 gggcatataa ccttacaata tctcctacag gtagaacttt atctggaggt attgaccctg
     8941 gtgcacttca tggacctaaa aagttctttg gtgcagcaag gaatattaga cagggtggtt
     9001 ctttgacaat attaggaaca gcattggttg aaactggttc taggatggat gatgtaatat
     9061 ttgaggaatt taaaggtaca ggaaatatgg agttacattt agatagaaaa ttagcagaaa
     9121 aaagaatatt ccctgcaata gatatttaca agtctggtac aagaagagat gatttattgc
     9181 ttgatgatga agaaaagact gctctttgga gattaagaag agaaatgagt aataattcag
     9241 taatggaaat tacagataaa gtcattgaac ttataaagag aactaaggac aataaagagt
     9301 ttgtaaaatc aataaagaat ttgtaaaatc aataatacaa atgcaaatat ggattatata
     9361 aactaataga aaaatcactt gaatggcata ataagttgtg ctataataat gtagttgata
     9421 atgtaaaaag aaagttaata taattaattt ttttaaataa agagaagagg tgactataat
     9481 gcaaaaggaa atacaaccaa aatacaatcc agttgaagtg cgttgtgctt gtgggaacac
     9541 atttgttgct ggttcaacta aggacgaaat aaaagttgaa atatgttcag agtgccatcc
     9601 attctataca ggaaaacaaa agaacataga aaaaggtgga agaatcgaca aattcaagaa
     9661 aagattcaaa atggactaat ttttataaaa gtggagttgg aggaagccaa ctcctatttt
     9721 attacaaaaa aacattcata ttctacttta aattgtcttt ttgtattata ataataaata
     9781 tgtaaaattg agtattctaa atacaataaa atgaggtgag tggatggcaa aacaagctgt
     9841 aggtgggcaa gctgttattg aaggtgtaat gatgcagtca aaagacaaaa gagtggtagc
     9901 tgttagaaaa agtgacggag aaatagcact aaaagaagat agaataaaaa gttgggtaag
     9961 agataagaat atagataaga taccttttgt tagaggttct tttgttatga tagatacaat
    10021 gattcaaggc ataaaaagtt taaacttttc ttcagagttt tttatggaag aagctgaaga
    10081 ggataaattt gatttattta taaagaaaat ttttaaagat aaggcaaatg atataattat
    10141 aatattttca ttggtcatag caatgttact ttctgctgga ttattcattt ttatacctac
    10201 attggtagga ggagcttttt ctaaagtaat gcctaatgat tttatgctta atttaataga
    10261 aggaatcata aggatagcta tattatttgc atatatagta ttgatttcac gaagtaagga
    10321 tatagaaaga gtctttcaat atcatggagc agaacataaa tctatatatt gctatgaaaa
    10381 tgatttagaa cttacagtag agaatgctag aaaatttaaa agattacatc ctagatgtgg
    10441 tacaaatttc ctatttatag ttatggctgt atcaataata ttgtttgcat tttttggttg
    10501 gccaaatcca attcttagaa tatttatgag aattatatgt gtgcctatag tagcaggttt
    10561 atcttatgag gtaattagag ttcttgggaa atatgataat ggatttacta aaattattgc
    10621 atatccagga atgatgttgc aatattttac aacaaaagag cctgatgatg agcaattaga
    10681 ggttgctttg gaagcattga aagcagtagt agattagaga aaaggatatt tgttatgacg
    10741 ataaaagata taattattaa atattcagat aaactaaaag atataagtga tactccaaga
    10801 ttagatacag agttattgtt acaaaaaaca ttgggtgttg atagattata tattcactta
    10861 aatctgaata aagagctaac tgaagaacaa aaaacaaaat ttatgggttt tgcagaagaa
    10921 agattaaatg gaagaccaat agcatatata gttgaaaata gggagtttat gggattagat
    10981 ttttttgtta aggaaggcgt attgattcca agaccagata cagaaacttt agtagaagaa
    11041 ataattgaaa tatgtagaga aaaaaaagat gtaagtatat tagatatagg aactggttct
    11101 ggagctatta caataagttt agcaaagtac attgaaaatt ctaaaatcat gtcttttgat
    11161 atatcagaga cagcattaga aatagctaaa aaaaatgcta ttataaatga agttggtgaa
    11221 aaaataaagt atattaattc tgatttattt acagcaataa gtgatagtaa cataaaattc
    11281 gatatcatag tttcaaatcc accatatatt aaaaaacaag atatagaaac tctgcacaaa
    11341 caagttaaag attatgaacc atataatgct cttgaaggtg gagaagatgg attagacttt
    11401 tatagaagaa taacagaaca aggtaagaaa tatttaaata agtgtggaat attagcatat
    11461 gaagttggtc ataatcaagc tgaagatgta ataaatatta tgaaaagtaa tggatataaa
    11521 aaaatatata caaagaagga tattcaaggc attgatagag ttgttatagg ttacaatata
    11581 tgatataatc aacaattgat atgttacatt taatcgaggt gaaagtatgt taaaaaaatt
    11641 agaggtattg gaagatacat ataaagactt aagtgaaaaa ataggtgatc cggatgtaat
    11701 aaatgatcaa aaagtttggc agaaatatat aaaagaacat gctgacttgg aacctattgt
    11761 tatgaaatac agagagtata aaagcgtttt agatagtata aaagaatcaa aagaaatttt
    11821 acaagaagag tcagatgaag aattaagaga attagctaaa atggagttag ctgagatgga
    11881 agaaaaagta gcacctctgg aggaagaaat aaaaatacta ttattgccta aagaccctaa
    11941 tgatgataag aacgttatag ttgaaattag aggtggagct ggaggagatg aagcagctct
    12001 atttgcgggt gatttattta gaatgtactc aaggtatgct gaaagaagaa gatggaaaat
    12061 agaattattg agtgcaagtg atactggagt tggtggatat aaagaagtat cttttatgat
    12121 aaaaggtaaa ggtgcttatt caagattaaa gtatgaatct ggagttcata gagttcaaag
    12181 aataccatca actgaatctg gtggaagaat acacacatca acttctactg ttgcagtatt
    12241 accagaagtt gaagatgtag aggttgaaat aaatccaaat gatttaagaa tagatgtttt
    12301 ccgttcttca ggtaatggag gacaaagtgt caatactact gactctgctg ttagggtaac
    12361 acatatacct acaggtgaag tagtatcttg tcaagatgga aaatcacaat taaaaaataa
    12421 agagcaagct ttaaaaatat taaaagctag actttacgat aaagcattgg cagaacaaca
    12481 caaagatata gcagcagaaa gaaaaagcca agtcggaaca ggagacaggt cagaaagaat
    12541 aagaacatat aatttccctc aaggtagaat aagtgaccat agaataaatt taactttata
    12601 taagttagat gcattcttag atggagatat agatgaaatg atagatgctt taattactgt
    12661 tgaccaaaca gaaaaaatga ctgcaatata agtaatgttt taaagaagct gtttcttcct
    12721 agaaattatt ttctattttg aaacagctat tttgcatttt aggtcatgtt atcatattct
    12781 attgtatata attgttatat acaagtatag gagggttata tgatactaaa agtaacaata
    12841 attgggcttt tggcaggtgt tatagggaca ggattaggag gagtaatatc agccattttt
    12901 aaaagagagg tcgataaata tctaagtttt tttatgggat tatctggtgg aataatgttg
    12961 gctgtagtgg tgtttgattt aatgaaagaa tccatggaca aaatggggat aatcaataca
    13021 gttatattta cgtttgttgg agctctcata actatgtaca taaaaacaaa attagatgtg
    13081 tctggaaata tggcatctgg atatcttata ttcataagta tattactaca taatttacca
    13141 gaaggactag ccattggttc ttcttttatg tcaactgaaa gtttaggtat tacacttgct
    13201 atagttatag gtctacataa tataccagaa gggttagcta tggcattagg cttagtttgc
    13261 aataaaatga aactaagtaa agttattttg tttactgtaa tagctggatt acctatgggt
    13321 ctaggaagtt ttttaggtgt ttattttgga ggagtattta cttctcttat aggagtattt
    13381 cttgctacag caggaggtac tatgatgtat gttgtattgg aagaaatttt tccacattca
    13441 aaaagtgtgt attgtataat tggtttcttg ttaggtacaa tgattgtaaa ttatatataa
    13501 aattaaaatg aaagcaggca tacaaaagat gaaaactatt ataagtaata ttgatataaa
    13561 taatattgat tatgaggaaa taaaaataca agcaaaactt cttagagaag gtaaaacagt
    13621 tatttttcca actgagacag tatatgggtt gggtgcaaat gcattagatg aaaatgcagt
    13681 aaagaagatt tatgaagcta aaggaagacc tagtgacaat ccattgatag tccatatata
    13741 tgaaaaagaa gaagtatatg acttagccaa agatataagt gataaagcta aacttgttat
    13801 agaaaagctt tggccaggac ctataacaat tatattaaat aaaaaagata taattccata
    13861 caaaactagt ggagggcttg atacagttgc aataaggatg ccttctaatg ttattgccag
    13921 ggcaataata aaagaagctg gtatacctat tgcagctcct tctgcaaata tctcaggtcg
    13981 cccatctcca acaaaagcaa aacatgtata tgaagagatg aatggaagag tggatggaat
    14041 agttttagga ggagacagta attttggatt ggagtcaact gtattggatt taacagaaga
    14101 gactcctatg atactgagac caggaagtat aactaaagaa gttttagagg aacttcttgg
    14161 agaagtaaag cttgacccat cattaagtaa aaaagaagat aatcaaaaag ctaaagctcc
    14221 tggaatgaaa tataaacatt attctccaaa tgcagatgta tatataatat ctggaaaaag
    14281 agaaaatgta gcaacaaaga ttaatgatat gataaaatct aatagagaca aaggtctaaa
    14341 gacaggtgta atgtgtatta gtagaaaccg agagttttat gatggagaag taatcgattt
    14401 aggaaactct cttgaagaag ttgcaagtaa tctgtttgat gctcttatag aaatggacaa
    14461 aaagggtgtt gatgtcatat attcagaaga gttccctaag actggagttg gacaagcaat
    14521 tatgaataga ctactgaagt cagcaggata caaagtgata aaggcttaag agggagttgt
    14581 aaaatatgaa tatacttatt gtgtgtacag gtaatacttg tagaagtcct atggctgagg
    14641 ctatactaag gaaagcaatt aaagaatctg gaagaagtat tgaagaatat tctatatcat
    14701 cagcaggtat atctacagca aatggaatgg gtgcttctga aaattctata gaagtactaa
    14761 aagaaatcgg tatagactta tcaaatcata gaagtaaagt aattactaaa aaattaattg
    14821 atgaatcaga tataatacta acaatgacta aatctcataa agaaatttta gtacaagcag
    14881 ttccaaaatg caaagaaaaa gtgtatactt ttaaagggtt tgcaaataaa aatgaggaag
    14941 atatatctga cccatttggt ggaaatttgg atatttatag aagcactatg agagaaataa
    15001 tgtattctgt caacgaaata gtaaaaaaaa tatgaaacgg aggataaaca aatgaagata
    15061 ggattaggct gtgaccatgg aggatataat ttaaaaaaag aaataataag ctatttagaa
    15121 agtaaaggta tagaatgtgt agattacgga actaataatg ctacagattc tgttgattat
    15181 cctgtttatg gagaaatagt tgcaaattca gttataaata aagaagtaga ttatggaata
    15241 ttatgttgtg gtacagggat aggaatatct ttagctgcta ataaggttcc tgggataaga
    15301 tgtgcagttg tatcagatgt attttctgca aaaatgtcaa aagcacataa tgatgcaaat
    15361 atgttatcat taggcgaaag agttttagga aaaggtctag cacttgagat agttgaagca
    15421 tggattaata cggattttga aggtgataga catgctagaa gagtaaacat gattaaatca
    15481 atagaagaaa aacataataa ataataaaaa ttacagtttt atcaggagga attatcaaaa
    15541 tgagcaaagt ggtagagaca aaccatccat taatacaaca taaattaact ttaatgagag
    15601 ataaaaatac tggttctaaa gactttagag agcttttaac tgaaattgca atgttaatgg
    15661 ggtatgaaat aacaaaagat ataccattaa aagatgtaga aatagaaact cctattcaaa
    15721 aaacatcatc taaagtagtt gcaggtaaaa aattagcaat aattccaatt ttaagagcag
    15781 gtcttggaat ggtagatgga ttagtgagct taatgccagc agcaaaagtt ggacatgtag
    15841 gactatatag agacccagaa acattaaaac cagttgaata ttattgtaag cttcctcaag
    15901 acataggaga aagagatata atagtagttg accctatgct tgcaacaggg ggttctgctg
    15961 tagcagctat agatttactt aagtctaaag gagctaaaag tataaaatta gctaatttag
    16021 tagctgcacc agaaggtata gcagaagtgc aaaaatatca tgatgatgtt gatatatatg
    16081 ttgcatcagt tgacgaaaga ttaaatgaac atggatatat aattcctggt cttggtgatg
    16141 ctggagatag attgtttgga actaaataaa aataaataac tataaatatt tattagggac
    16201 tttcttatat agtgtatata attttatgta agaaggttct tttttatata ttttttctca
    16261 aaataggaat tattgaattg ataaatttct cgaattgaga ataaaaagtt tttaccaatt
    16321 aaaaaagatt gcttatttta caaggtttta tagaatagta gtctatttta atgtggcatg
    16381 ttagttgctt acgattaaga ttataggtat taagtgtatt agttaaattg agaattgaat
    16441 attaataata ttattgggag tggtaataca tgaagaattg tatagtagta agaggggctg
    16501 gagatttagc aacagcagtt attcataaat tacacaacag tggttttaaa gtagttgcgt
    16561 tagaaataga agagccatta gctataagaa ggcaaatttc tttttgtgag gctgtttatg
    16621 aaaaaaaagt aactgttgaa ggtgtagaat gtatattttg caatactgaa aaagaaatag
    16681 aagaagtttt atctcaacat aaagtagcct tgattataga cccaaaagga gaaaaattat
    16741 ctaaattaaa tccaatcgtt ttggttgatg gaatactcgc taagaaaaac ttaggtacaa
    16801 ctaaagatat ggcaccattt acaatagctt taggtcctgg attttgtgca ggagaagatg
    16861 tagatgttgt tatagagaca atgagaggtc ataatctagg aagaataata actaatggta
    16921 gagcagaaaa aaatacagga gtaccaggag caataaaagg tttttctaaa gagagagtta
    16981 tgcattcaca gtatgatggc ataatggaaa atgtatgtaa gataggtgat atagttgaaa
    17041 agggagacct actttgctat attaataatt atgaaaaaaa acatgaagtt agagcaacaa
    17101 tatctggagt ccttagaggt ttattaaagg atggagcaaa ggtaaacaaa aatttaaaaa
    17161 tactagacat agaccctaga aaagaagaag ttaagaatgc ttttacaata tcagataaag
    17221 ctagatgtat agctggaggg gttttagaag ccatactaaa caatgggatt tatccccaaa
    17281 attaatttat attagataaa gctattaata ttttattaag atgatataat aaatttaata
    17341 gattaaagag agcatatgac gggtatttaa aatttaaatg gtaaaaatgc tcttttttta
    17401 ttggacattc aattaacatg tgtatataat ataaaaataa gtatttattt aggttgaaat
    17461 ctaataaata tataatacca gtacataagc aaagaggaga aaagtaaatt atgagaccat
    17521 catgggatga atacttcatg gaaattgcag aagtagtgaa aaagcgttca acttgtatta
    17581 gaagacaagt aggagctgta attgtaagag acaaacaaat attaacaact ggatataatg
    17641 gttctccgag aaatcttgaa cactgtgaaa atattggatg taagaggcag gaattaaata
    17701 ttccctcagg agaaaggcat gaattatgca gagcattaca tgctgaacaa aatgcgataa
    17761 tacaagctgc tcataatggt attagtgtag atggggcaac tttgtatgtt actacaagac
    17821 cttgtgtttt gtgtgcaaaa atgtgcataa atgcaggtat attgaaaatt gtttatgaag
    17881 gtgattatcc agatgatatg tctacagaat tattaaaaga agcaggaata gaactcataa
    17941 aattttagtg gataaaattt ataagacaat aaatgtcaaa ttatgacact gaaatacaat
    18001 gatatcaaat gattaaaaga aagtatttga aaaatcatca gattagtagt aagatatatt
    18061 ttataattaa ataaatttat gatatagatt aattacatgt tatttatttt ataataatga
    18121 gggatactat ttatgagtaa aagaaataca catacagaaa ttgcaaagtt gtttagttta
    18181 ataagtcaaa taggattgat gattgtaata tcaatattag gatgtacttt cttaggaaaa
    18241 tttttagata gtaaattgaa tactactcca gttctgacaa taatattttt attgttgggt
    18301 gttggaggtg cttttagtgg tgtttataaa actctaatag tatatacacg aaggaagtga
    18361 tatattggat ataaaattac ttaaccaggt aaggtcagta acaaaaggta tagtaatatt
    18421 tgatgtagtt ttaattgtgc taatgttgat aacatcaaca ttaagtaaac cagtattatt
    18481 aggacttata tttggctcta taatagctat gttaaatttt agattattgg cgatatcact
    18541 ggaaaaatca gtgacatttc ctgctgggaa ggcacaagct tatgcatcag cacaatatgc
    18601 tgttagatta tttataatgg cagttgttct aattgcatca gctacagtac cacatttaaa
    18661 tataatcgga acagcattgg gattattgag cacaaagttt gtcatattat caaaaaactt
    18721 tataaataaa ttaaagagga aggaggcata agtatgaatc aagcgcatta ttatctcata
    18781 ggaaacttta agattagtga aacaatacta gtaagttggg gaataatggc ctttttagcg
    18841 atagcatctt attttttgac tagaaactta aaaaaagttc caactagtaa agttcaaata
    18901 ttcttagaat ttgcagtagg tggtcttgct aaaatggtta cagatgttat gggtaaaaat
    18961 acagtaaaaa gaatgccata tatgatacca tacataggaa gcttgttttt atttttcgca
    19021 tgttcaaatt taagcccatt acttggatta aggtcaccaa caaccgactt agacacaact
    19081 gctgcatggg caatgattac attttttatg atttattttg caggagttaa atttaagggg
    19141 ttatcatact ttaaagaatt ggctcaacca cttgccataa tgttaccaat caatattata
    19201 ggtgagttag ctagaccaat atcattaagt ttccgtccat ttgggaatat tttaggtgga
    19261 acaataataa tgggtctgtt ttatcaactt ttaggtttta tatctagttt aataccagga
    19321 ttatcaatac caattggaga aatattaata ccagtcccat tacacttgta ctttgactta
    19381 tttgcaggat tgttacaagc atatatattt ataatgttaa caatggtatt tatatcaagt
    19441 gcaggagagt tagattaaga aatcaactta tattaagata aaaataaaat aatttaaaaa
    19501 attaaagttt taaggaggaa ataattatgg aaacagcaat agtagcggca gcatcagcaa
    19561 taggagcagg gatagcagtt gcaacaggta taggtgcagg tataggtcaa ggtatagcag
    19621 cagctaaagc tgcagaagca gtaggaaacc agccagaagc taaaggtgat ataacatcaa
    19681 ctttattact aggagttgct atagcagagt cttcagcaat atatggtttg gttatatcaa
    19741 taatacttct attcgtaaat ccattcttta aatacttagg aatgtagatt tagtccttaa
    19801 atattttgag ataggtttat aggtaaatca tctcaaactt ataatcatga gatgaacttt
    19861 atcatctcgt gattataagt agttatgatt tatggaaagg aggataatta tggaaaaagc
    19921 actagtaggt ataacgtggg aatttgtgtt ccaaattgtt aatactttta taatattttt
    19981 acttctgaga aagcttttat tcaagccagt attaaatata attgaatcaa gagaaaatga
    20041 tattaaaagt gatttggcag aaggcgaaaa agcaaaaaat gaaggtttag ctctgaaaaa
    20101 agaatatgag tcaaaaatca attttgccaa agatgaaggt caagaaatca taaaacaagc
    20161 aactataaga gcagagcaga aatcagatga tatagtaaat actgcaaaga aagatgctct
    20221 tgatataaaa gaaaaagcaa acaaagatat agaacaagaa agacaaaagg ttataaatga
    20281 aatcaaaaat gatatatcta acatagcact acttgcagca tctaaagtta ttgaaaaaga
    20341 tttagataaa tctaaacatg aagaattgat agaaaacttt ataaaagagg taggggaagc
    20401 taaatgataa atgtaatagc aaatagatat gcagaagccc tatttcaatt aggagaagaa
    20461 gaaaattcaa ctgatgtctt attcaaagaa ctagaaaaag tagtagatat gatgactaaa
    20521 gttagtaaag atttctacaa agttttaaaa tcaccattag tttcaaaatc agaaaagaaa
    20581 aacttggtag aaattatttt tagtaaagaa gtaagttcta acataaaaaa ctttttaaaa
    20641 gttttagtag ataaagatag aatatcatat ttagaagata tcgaattagc ttataaagaa
    20701 ttattaaata agaaaaacaa tgtaattgat ggagtagcaa taagtgctat tccaatgagt
    20761 gaaacagata ttaaagaact tgaagtcaaa ctttcaaata aatacaacaa aaatgttact
    20821 atagaaaatg ttgttgacaa gactatacta gggggagttc tagttagaat tggaaatgaa
    20881 caaatagatg gcactgtgaa aactcgtctt gataagatga aagaaaagct atctgaagta
    20941 atatcttaag aatggcggtg aacccatgaa cttaaaacct gaagaaataa gttctataat
    21001 taaacagcaa ataaaaaatt atgagaataa agttgagttg acagatacag gtagtgtttt
    21061 aacagttggg gatggtatag cgagtgtata tggattagaa aaagctatgt ctggtgagtt
    21121 gctagagttc ccaggtgaaa tatatggtat ggcacttaac ttggaagaag aagtagttgg
    21181 agctgttatc cttggagatg acagtgaaat aaaagaaggc gatatagtta agagaactgg
    21241 aagaatagtt gaagttccag ttggagaagc tttaataggt agagttgtaa actcactagg
    21301 tcagccaata gatggaaaag gacctatagc gtacacaaaa actagaccag ttgaatctga
    21361 ggcacctggt ataatagata gaaggtctgt ttatgaacca ttacaaacag gaatcaaatc
    21421 aatagattca atgataccaa ttggtagagg tcaaagagag ttaataatag gtgacagaca
    21481 aactggtaaa acatctatag ttattgacac tatactaaat caaaaaggaa aagatgttat
    21541 ctgtatatat gtagcaattg gacaaaaacg ttctactata gctcaattag ttagctcttt
    21601 agaaaaaggt ggagctttgg attatacaat agtagtttca gcaacagcat ctgaatcagc
    21661 accacttcaa tatatagcac catatgctgg agcagctatg ggtgaagaat ttatgtacaa
    21721 tgggaaacat gtacttatag tgtatgatga tttaagtaaa caagctgttg catatagaga
    21781 aatgtcattg ttacttagaa gaccaccagg acgtgaggca tatcctggag atgtattcta
    21841 tttacattca agattacttg aaagagcagc taagttatct gatgaattgg gtggaggttc
    21901 aatgactgcc ttgcctataa tagaaactca agctggtgac gtttctgcat atataccaac
    21961 aaatgttata tctataacag atggtcaaat atacttacaa ccagagttat tctattcagg
    22021 tgtaagacca gcagttgacc ctggtatatc agtatcaagg gttggtggtt ctgcgcaaat
    22081 taaagcaatg aaaaaagttg caggaacatt aaaacttgca tattcacaat atagagaact
    22141 tgcagcattc tcacagtttg gttctgactt agatgaagat actaaaaaga gacttgctca
    22201 aggtgaaaga atcgttgaga tattaaaaca aggtgagcat caaccaataa aagttgaaaa
    22261 tcaagttatg ataatatatg ctgttataaa taatcattta gaggatattc cgatagataa
    22321 tatagctaga tttgaatcag aattgtatgc atttgtagat aataattatc cggaaatatc
    22381 aagaaaaata ttaggtggag aagacttcac tcatgattta acagatgcaa taaatgaatt
    22441 taaagaaaag tttgttgttg aagtataatc ctttttaaaa aaggaggtaa attaattggc
    22501 aggagctgga atcaaagcaa tcaaaacacg tatgggaact gttgaaagta caaagcaaat
    22561 aactaaagca atggaattag ttgcatcttc taagcttaga aaagcaaaag aaaaggctga
    22621 agattcaaga gaatatttta atactttata caatacagta aaagatattg cccaaacaac
    22681 acgtggaatt agaaatgaat ttttaaaaga aagagaagta aaaaataaat gctatgtagt
    22741 agtagctgga gacagaggac ttgcaggtgg atacaattct aatgtattaa aggagtctgt
    22801 ctctcatatg aaaggtaaaa aggaaacagt gattactgtt ggaaagaaag cacatgaatt
    22861 tttttctaaa agaaactaca atatagcaaa agatataaat agtgttgaag cttgtggata
    22921 tgaagatgcg acagaaattt cacaaactat tattgacctg tataaaaaag gtgaagtaga
    22981 tgaagtttat attgcttata caaaatttaa atctgcttta tcacaagaag ttgaaatttt
    23041 gaaattgctt ccattagtct ttgaaaaaga tacagacgaa caaggaatgg cagtaaaaga
    23101 agaaaaacaa aatagatcaa aagtacaata tataccatca gcagaagcag ttttaagtta
    23161 tattgtacct cagtttatat caggtacagt atatggtgga attttggaat cttttgcatc
    23221 agagcaaggt gctagaagaa ctgcaatgga atcagccaca gacaatgcga atgaaatgct
    23281 atctaattta gagttaagtt ataacagagc aagacagtca gctgtaactc aagaaataac
    23341 agagatagtt ggtggagttg aagctctaaa atagggaggt taggaaatgg caaatgtagg
    23401 taaagtagta cagattgtag gagctgtact agatgttaag tttgatagtg aacaaagcct
    23461 acctaactta ttaaatgcgt tagtaataaa gttaggagat aaagaaatag ttgcagaagt
    23521 tgctcaacat ataggtgatg atactgtaag atgtatagct atgagtgcaa cagacggact
    23581 tgtaagaggt atggaagttg tagatacagg agggccaata agtgttcctg ttggagatga
    23641 aactttaggt agaatattca atgttcttgg aaaaccagtt gatgggaaac cagctccaaa
    23701 atctgcacct aaattaccta tacatagacc agcaccggcg tatgatgaac tagaaactac
    23761 agcagagata ctagaaactg gtattaaggt agttgactta ctagctccat atctaaaagg
    23821 tggaaaaata ggtctatttg gaggagcagg agtaggaaag acagttctaa tacaagagct
    23881 tataaacaat attgctaagc aacatggtgg tatttctgta ttttcaggag taggagaaag
    23941 aacaagagaa ggtaacgacc tttatggcga aatgagtgag tctggagtta taaataaaac
    24001 agctctagta tttggtcaaa tgaatgaacc acctggagcg agaatgagag ttgctttaac
    24061 tggacttaca atggcagaac attttagaga tgagcaagga caagacgttt tacttttcgt
    24121 tgataatata ttccgtttca cacaagctgg ttcagaagtt tcagcacttc taggacgtat
    24181 gccatcagct gttggttatc agccaacatt agctactgaa atgggtgcac ttcaagagag
    24241 aataacatca actaagaaag gttcaataac atctgttcaa gcagtatatg taccagcaga
    24301 tgacttaact gacccagcac cagctacaac attctctcac ttagatgcaa aaacagtttt
    24361 atcaagacaa atatcatcac tgggtatata tccagcagtt gaccctcttg aatctacatc
    24421 aaggattctt gaccctagta tagtaggaaa agaacattat gaagtagcta gaggtgtgca
    24481 atcaatactt caaagatata aagaacttca agatataatt gctatacttg gtatggatga
    24541 attatctgat gaagataagt taatagttgc acgtgctaga aagatacaaa gattcttatc
    24601 tcaatcattc acagttgctg agcaatttac aggaaatcca ggtcaatatg ttcctgttaa
    24661 agagactgta agaggattta aagaaattct tgaagggaaa catgatgact taccagagtc
    24721 agcattctta tttgtaggaa caatagaaga cgctgttaga aaagcgaagg gaagtatgta
    24781 gtcatggcca gtgaattttc attgaaagtt gtaactccaa gtaatacttt ttatgatgga
    24841 gatgttgaaa tgatagtagt tagaactaca aaaggagaca aaggggtgtt gaaaggtcat
    24901 ataccatttg tttcaggtat acctgtagga actttaaaga taaaaaaaga gggaagtttt
    24961 aaagaggcga aaatagcaga aggattcatt aatgtaactc aagataaaac tgtcatattg
    25021 acagaatctg ctgagtggat ttaataaatt aatttagagt ataaaaagtc attttgacca
    25081 aaaaaaactt ttgggtaaaa tggctttttt gcgttttggt gttaataaaa taacgataaa
    25141 attgtaagtt tttagtgata ataaattcac aaaatgttta aatttactgc caaattatgt
    25201 tgtaattaat aagataaaga agatttagat agcttttatg taagtgaaaa agtcttcata
    25261 taagaaatac atataaaatt tttataaaat tt
//
LOCUS       DAKBNN010000035        25232 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000035 DAKBNN010000000
VERSION     DAKBNN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.035"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            56..1507
                     /locus_tag="OUB02_001267"
     CDS             56..1507
                     /locus_tag="OUB02_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCT8885038.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1727..2188)
                     /locus_tag="OUB02_001268"
     CDS             complement(1727..2188)
                     /locus_tag="OUB02_001268"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCT8885039.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2293..3486)
                     /locus_tag="OUB02_001269"
     CDS             complement(2293..3486)
                     /locus_tag="OUB02_001269"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HCT8885040.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3509..4174)
                     /locus_tag="OUB02_001270"
     CDS             complement(3509..4174)
                     /locus_tag="OUB02_001270"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCT8885041.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4208..5320)
                     /gene="ribD"
                     /locus_tag="OUB02_001271"
     CDS             complement(4208..5320)
                     /gene="ribD"
                     /locus_tag="OUB02_001271"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCT8885042.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5452..5573)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5762..8245
                     /gene="recQ"
                     /locus_tag="OUB02_001272"
     CDS             5762..8245
                     /gene="recQ"
                     /locus_tag="OUB02_001272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCT8885043.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAAIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8334..8445
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8587..9888
                     /gene="thiC"
                     /locus_tag="OUB02_001273"
     CDS             8587..9888
                     /gene="thiC"
                     /locus_tag="OUB02_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCT8885044.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9942..10136
                     /gene="thiS"
                     /locus_tag="OUB02_001274"
     CDS             9942..10136
                     /gene="thiS"
                     /locus_tag="OUB02_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCT8885045.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10129..10932
                     /gene="thiF"
                     /locus_tag="OUB02_001275"
     CDS             10129..10932
                     /gene="thiF"
                     /locus_tag="OUB02_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HCT8885046.1"
                     /translation="MVKIKIFVNELLTDVEENITAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10995..11765
                     /locus_tag="OUB02_001276"
     CDS             10995..11765
                     /locus_tag="OUB02_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCT8885047.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11782..12885
                     /gene="thiH"
                     /locus_tag="OUB02_001277"
     CDS             11782..12885
                     /gene="thiH"
                     /locus_tag="OUB02_001277"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCT8885048.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12886..13575
                     /locus_tag="OUB02_001278"
     CDS             12886..13575
                     /locus_tag="OUB02_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCT8885049.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13763..15157
                     /gene="yfcC"
                     /locus_tag="OUB02_001279"
     CDS             13763..15157
                     /gene="yfcC"
                     /locus_tag="OUB02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCT8885050.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15674..17041
                     /locus_tag="OUB02_001280"
     CDS             15674..17041
                     /locus_tag="OUB02_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCT8885051.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17066..17674
                     /locus_tag="OUB02_001281"
     CDS             17066..17674
                     /locus_tag="OUB02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HCT8885052.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            17722..18093
                     /locus_tag="OUB02_001282"
     CDS             17722..18093
                     /locus_tag="OUB02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCT8885053.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18272..18610
                     /locus_tag="OUB02_001283"
     CDS             18272..18610
                     /locus_tag="OUB02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCT8885054.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAKAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19077..19580
                     /locus_tag="OUB02_001284"
     CDS             19077..19580
                     /locus_tag="OUB02_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HCT8885055.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19627..20586
                     /gene="moaA"
                     /locus_tag="OUB02_001285"
     CDS             19627..20586
                     /gene="moaA"
                     /locus_tag="OUB02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCT8885056.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLLENKCSDTNRRMYEIGG"
     gene            20590..21060
                     /gene="moaC"
                     /locus_tag="OUB02_001286"
     CDS             20590..21060
                     /gene="moaC"
                     /locus_tag="OUB02_001286"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCT8885057.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21096..21527
                     /locus_tag="OUB02_001287"
     CDS             21096..21527
                     /locus_tag="OUB02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCT8885058.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
     misc_binding    21958..22169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            22332..23621
                     /locus_tag="OUB02_001288"
     CDS             22332..23621
                     /locus_tag="OUB02_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCT8885059.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            23675..24754
                     /locus_tag="OUB02_001289"
     CDS             23675..24754
                     /locus_tag="OUB02_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HCT8885060.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            24775..25140
                     /locus_tag="OUB02_001290"
                     /pseudo
     CDS             24775..25140
                     /locus_tag="OUB02_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT         9658 a         2714 c         4441 g         8419 t
ORIGIN      
        1 tttgatttta agatttattg agaaaacaaa actaaatagg ggggtaatta atataatgga
       61 ttttttcaat ggtattttaa cgagtattaa caatgcagta gtatatgtaa acaattactt
      121 atggtcgtat atcttaatta taatgttagt tgtaattggt atttacttta ctattaaaac
      181 aaattttgta caattcagat attttataga aatgtttaga cttctgggtg atggtacagc
      241 gaataaaaat gcaaaaaagg aaggaaaaat ctcttcattt caagcatttt gtataagtac
      301 atcttcaaga gttggaactg gaaatatagc tggaatagca attgcagtgg tagcaggagg
      361 acctggtgct gtattttgga tgtggcttat agcattaatt ggttctgcat caagctttgt
      421 tgagagtaca ttagctcaga tttacaaagt gaaagatggt caagctttta gaggtggacc
      481 agcgtattat atggaacaag gtctaaataa aaaatggatg ggtattttat tctcaatact
      541 tataactata tgttatggtt ttgtatttaa tgcagttcaa gcaaatactg tttcacttgc
      601 atttaataat gcatttggaa taagtaaaat ggctatggga ataggtcttg gaattttaac
      661 tgcaatagtt atttttggtg gagtgcatag agttgcaaaa gtttctgaaa taatagttcc
      721 tgtatttgca ggattatata tattagttgc tttggctata gttgtaatga atataacaga
      781 gataccaagt gttatagcat tgatagttga aagtgcattt gattttaaag gaatggcgat
      841 tggtacgttt atgggagttg taatgactgg agtgaagaga ggtctatttt ctaatgaagc
      901 aggtatgggt agtgctccta atgctgctgc aacagcacat gtaactcatc ctgtaaaaca
      961 aggtttaatt caatctttag gtgtttttac agatactata ataatatgta gttgtacagc
     1021 attcatggta ttattatatt caggttatag tagtagtgga gcaactggaa ttgaattagc
     1081 tcaagaagct ttaactcatc atataggacc agttggaaat atatttatag cagtttgtat
     1141 attcttgttt gcatttagtt ctatagttgg taattactat tatggtgaat caaatatgga
     1201 gtttatgagt ggaagtaaga ctaaacttaa tgtatttaga gtttttgttg taggtatggt
     1261 tttatttggt tcacttactc aagtagatgt agtttggaat cttgcagact tatttatggc
     1321 attgatggcg ataataaact taatagcaat agcactttta ggaaaatatg catttattgc
     1381 tttaaaagat tatacaagtc aaaaaaaatc aggaataaaa gaccctgtgt ttattgcaga
     1441 tgaaatagaa ggcctagaaa aggtatcaga gtggcatagg gaattatctg aggaacattt
     1501 aggataaaaa atagtataaa attataaaac cattttgaaa gactagagta tgtatatact
     1561 ttatatgaat ataatattga attaatttat attactggaa tttttaattc cagtaatatt
     1621 tttttattat ataatttagc tttattaaaa gtttttatta ttaaaaatag cttatttatc
     1681 gtaaagtgaa tgatattggg cttttaatta ttatgaatgt tagtatttaa tttaaagact
     1741 taaataaatt tgccatttct agcgcattca tagcacaatc ataaccttta tttccagctt
     1801 ttgtacctgc tctttcaata gcttgttcaa tattttctgt agttacgatt ccaaatataa
     1861 caggtaattc actgtctaaa gatactttgg ctatcccttt agagacttca ctacatacat
     1921 aatcataatg agaagtagcc cctcttataa cacagcctaa acagattata gcatcatact
     1981 taccactttt agccattttt tgagcaacaa gaggaatctc aaaagctcca ggtacccaaa
     2041 ctatttctat attttctgga gaaacgtcat gtcttaaaag acagtcttca gctcctgata
     2101 aaagttttga tgttataaat tcattaaaac gagaatttat tatacctatt tttaaatctt
     2161 ttccaataag ttttccttca tatatcattt ttatttcctc caattttatt tatgttatag
     2221 atttatattt ataaatatct ttaatttaat tataaaaaac tacttagtag aaaaatatca
     2281 tcatataatc atttatataa tatctaaaag atgtcccatt ttatcttttt tagtttttaa
     2341 atatagctta ttaaagtcat taatttcttc attaataggg attctatttt cgatttttat
     2401 tccatatttc tctaattgat ttattttatc tgggttatta cttaaaagat ttatactttt
     2461 tatgtttaaa ttttttaata tttgagcagc catatagaaa tctctcatat cttcttcaaa
     2521 tccaagcatt aaatttgctt caacagtatc gtaaccttct tcttgaagtt tataagcctt
     2581 aattttattt aaaagaccaa tacctctacc ttcttgcttc atataaatta aaactccact
     2641 tccattttcc acaattttct tcatagaaga ttctagctgt aatccacaat cacattttag
     2701 agagtggaac acatctcctg ttaaacattc agaatgaagt ctaactagag tattttctat
     2761 atttatattt ccataagtaa gggcaatgtg ttcttcattt gaataagtat ctctatatcc
     2821 tatgatttca aaatttccat attttgtcgg aaggaaagca ctagatactt tttctatagt
     2881 agtttcattt attttgcggt attctataag gtcttttata gtgatgattt ttaaattatg
     2941 cttttctttg aaaatcatta aatcatcaac tcttgccatt gtaccatcat ctttcattat
     3001 ttcacagata agaccaatct ctttaaaacc tgcaagtcta gcaaggtcta ctgaggcttc
     3061 tgtatgtcca tccctgacaa gaactccacc agtttttgct attaaaggaa agatgtgacc
     3121 aggcgttttg aaatctttac ttgtagaatt ttcatcacag aataatttta cagtttcagc
     3181 tctttcatat gcagatatac cagttagagt atctgctcca tctatagtaa cagtaaaggc
     3241 tgtttgaaat gtatctgtat tttctctaac cataagaggt atattaagac ttttaaattt
     3301 tttttcagtg gcaggtagac aaattagacc tctaccatat gtagccataa aatttattga
     3361 ttcaggagta gccatttcag ctgccatcaa taaatctcct tcattctctc tactttcatc
     3421 atcaactacg attaccattt tcccattttt tatatcttca atagcttctt caatagtatt
     3481 aaacatgtaa ttcactcctt tattattttt aaaaaccatt tttaaataaa aaatctttat
     3541 ctatatttga tttatggtta ctactattag aatttaattc cttgtagtta ttagccataa
     3601 aattattaac atactttcca attacatcgc tttctaaatt aacgaaatct ccaacattct
     3661 ttgtcaagag aatagtgtta attttagtat gaggtataat agaaacctta aatatttcat
     3721 catctaaata tgatatagtt aaacttacac catcaatagc aacagaacct ttgagtatca
     3781 ttgaggataa aagtttttta tcaggtcgta tacttactaa aactgcattt tcatcttttt
     3841 caaatttgca gatagttcct ttaccatcaa catgacctgt tactatatga ccaccaaatc
     3901 ttgaagaaag actcatagct ctttcaagat ttaccttgtc atttgcattt aataaaccta
     3961 aatttgtgga acgaattgtc tccatcataa catcggctgt aaattcattt tttccgaggt
     4021 ttgaaacagt tagacaaact ccatttacgg ctatactatc tccaagattt gtgccatcta
     4081 atattttatt tgatagtatg gttacttttc cactcttacc attagcagta atttttctaa
     4141 gtattccaac ttcttctact atgccagtaa acatcctaac ctcctatatg aatgtattaa
     4201 caaaaattta atttaaaaca tctgattcta taagaatatc gccatctatc aaagttacat
     4261 tattgattgt taactgtgtt gaatctgaca aatgatttat tccttctcca gaaacgaatg
     4321 taggtgcatc tttaccacct ataatcttgg gtgatagaaa taatttgact ttatcaacta
     4381 acttatttct aaagagactg tcattgagag tagcaccacc ttcaactaga acagaatcaa
     4441 tgtttaactc tcctaattta tacattacaa actccaaatc tactaagttg ttttttgaag
     4501 gagaaattat tgtttcaaca ttattttctt ttaacataga taattttata ctttctttgc
     4561 aacatgtgaa aactatagtt ttactggttt tatctttaac caaattacta ttaagtggca
     4621 actttaggtg tgtatctatt actattcttg taggatgaga cactttttct tgattgagcc
     4681 tacaagttag taaaggatta tcgttcaaaa ctgtatttat gccaaccatt atactttggt
     4741 atttatttct gtatttatga gttagatacc ttgatgattc attagatatc cattttgatt
     4801 ctagtgattt tgtggcaatt ttaccatcta gacttactgc tgattttaca atacaaagag
     4861 gtcttttgtt ttttatatga taaaaaaatg cctcatttaa atctcttgct tcatcttcta
     4921 atattcccac agtaacatca atattgttat cttttaattt ttttactcca ttaccacaaa
     4981 caagtggatt tgggtctaat gtagatatta caactctctt aatcttattt tgtataattt
     5041 tatctacaca aggaggcgtt ttcccataat gagaacatgg ttctaagttt acataaagag
     5101 tagagtcttt tagactttgt ttagcactgt ttatagcatt tacttcagca tgattagagc
     5161 caaatttctc atgatatcct ttgcctatta tattactatc tttaacaatt acacacccaa
     5221 ctaatggatt tggatttaca aagccttctc catttttagc caattcaata gcttttttca
     5281 tataataaat atctttttct ttttgattca caaaattcac aatatgtaca cctcctgttt
     5341 aattggataa tttttaaaca aaaaaccctg agaattaact cagggtttag aataatctac
     5401 aataaatgtt taataaatgt agaacaaaat tgctctacaa aaacactcat taaatatctt
     5461 cttccatcca gactttactg tcggctttgg aatttaacca aatcaaccaa gttttacatt
     5521 ggctcgcggg ctataccgcc gattgggaat ttcaccctac cccgaagatt aagtattaaa
     5581 tttatattta gaatgtatca taaattattt tactttgcaa actatttttt taaaaaatat
     5641 atgttaatat ataatataga atgtctataa actttgaaat accacgattg tgaatataaa
     5701 aataaaaatt tgaacataat caggaaagaa gtctaattaa tttaaattag gagagtaata
     5761 tatgaataaa aaacttttag aaatactatc aaaatactat ggatatacaa gttttagaaa
     5821 aggtcaggaa tctataataa attctatctt aagcgaaaaa gatgtgctag ctattatgcc
     5881 aacaggaggg ggaaaatcaa tatgttatca aattccagct ttgattttag atggaatgac
     5941 aatagtaatt tctccactta tatcacttat gaaagaccaa gtagatgcct taaagactat
     6001 gggaattagt gctgctttta taaatagttc attaagcagc aaagaattta atgaaatatt
     6061 aaataatatt agaaaaaata attataaaat actctatatt gctccagaga gattagatgc
     6121 acaagagttt attgagctaa taaataataa caatgtaagt caagttgcga tagatgaagc
     6181 acattgtgta tctcaatggg ggcatgattt taggttgagt tatagaagga tttctgattt
     6241 tataaaaaat ttgcctaaaa gacctatagt tacagcattt actgcaacag cttcagaaga
     6301 ggtaagaact gatataataa atttactgtg tttagaaaat ccagattatt atataactgg
     6361 atttgataga gagaatttat caataaatat agtaaaatca tctagtaaaa ataagtatat
     6421 tttagattat attcaaaatc ataaaaatga gtctggaatt atatatgttg caactagaaa
     6481 ggaagttgaa aatatatata atggtttatc taagagaaat atatctgtat caaaatatca
     6541 tgcaggttta tctaaaaatg aaagaagtaa aaatcaagaa gattttataa atgataatgt
     6601 ggatataatg gtagcaacaa atgcatttgg aatgggtatt gataaaccta atataagatg
     6661 ggttatacat tacaatatgc ctcaaagtat agaaaattac tatcaagaaa taggtagagc
     6721 tggtagagat ggagaaaaaa gtgagtgtgt acttttattt tctccaggag atgtacatat
     6781 acaaaaatat ttaattgata ttggagttga aaatcctgaa agaaaattat ttcagcataa
     6841 gaagcttcaa tacatgattg acttagttta cagtaattca tgctatagaa agacaatatt
     6901 gagttatttt ggagaaaatt atatagacaa ttgcaataat tgtagcaatt gtttagtaga
     6961 gggtgaaata gtagataaaa ctatagatgc acaaaaagtc atttcatgtg tagctagaat
     7021 gaaaagaagt tatggtgtta ctactattgt agatgtgctt agaggttcta agaataaaaa
     7081 gattttacag cttggattaa atactttgtc tacatataac attatgagag attattctag
     7141 tgaagactta aagaatttta taaatacatt ggtttctcat ggttttttag atttagttga
     7201 gactctaggc aatggaaata gaggtagttt tcctactata agattaaatg agatgtctat
     7261 gaaagtttta aaaggtgaag tgaaagtaga gtttaaagaa attgttatga caaaatcatt
     7321 ggaagtagag gatgagttat ataatgcttt aagagaactt agacattcaa ttgcaagtga
     7381 agaaaggata gctccatata tggtatttgg agatggtaca cttagagcca tgtctagtag
     7441 ttatccaatt aataaagaag aaatgctaga tatctctgga gtaggagaaa ttaaatatca
     7501 aaaatatggt agagattttg aagcagcaat taaagaatat gtcgaaaaaa ataatataaa
     7561 taaaaaaata gatgaaaaaa atgtcgattc aaagaacgtt aatagtgatt tcttagaggt
     7621 taatacagat aaagttttat atgaaaaact gttagtcatt agagaagaat atgcaacaaa
     7681 ggaaaaatta cctgcatata tgattttagc caataaggct ctgaaagaaa taagtggaag
     7741 atatccactt gatgaagaac aattaaaaga tatttctggc ataggtggag taaaatttga
     7801 aaaatatgga gctagtattt tggcagaggt taataaatat gtaaaagaaa ataatattaa
     7861 tgttatttgg gagaaaaagg gcaaaaggaa gttaatatta gatggagaaa gtagaaaaaa
     7921 taatgaaata gcattagatt tgctaaatca aggaaaagat atacaaagta tcagtcaaga
     7981 aatagaagtt tcactgtcaa ccattattgg atatgtgcat gactatgtaa aggatggaca
     8041 tagtatatca tttgaattag gactagaaaa tttttataat gaagataaaa agaagatgat
     8101 tttagaagtt tgtgaaaaag ttggctatga taatttaaat aacataaaaa gaaaacttcc
     8161 agattcaata aagtatgaaa atataagagc tgtgatactt gactcttatc ttgaaaactt
     8221 aaaaaataat attatagcag agtaattttt aacttaataa aaaataaaat ttgtattatt
     8281 gtgttgaact aataattttg ctatgttata ctgaatttgt aaaaataatg aaaaattaga
     8341 gctaggggcg ctagaattgg ctagctgaga gataaagccg taacttacaa accctaataa
     8401 cctgatcaag ataatgcttg cgaagggaag ctttttaata taattttatt atataaatag
     8461 gcaaattatt caatgttagg agaaatttgt ctaagtgtat atacgattat aagagtgctt
     8521 tccaaacgag gaaaagcact ttttttattt tcaaaaacta taactttaat cttggaggat
     8581 tgtaatatga actatacaac acaaatggat gctgctagaa aaggcataat aactaaagaa
     8641 atggaaatcg tttctcaaaa agaacaggta gatgtaaatg aattaagaga attaatagca
     8701 aatggacaag ttgttatacc agctaataaa aatcataaat ctttaagtgc agaaggtgta
     8761 ggtaaaaatt taagaacaaa aataaatgta aatctaggaa tttcaagaga ttgtaaggat
     8821 atagaaaaag aacttgaaaa agttagagta gcaatagaca tgaaagctga agctataatg
     8881 gatttaagta attatggcaa aactagagaa tttagagaaa aggttgttga aatgtcacct
     8941 gcaatgatag gttcagtacc tatgtatgat gcagtaggat atttggaaaa agaattaaaa
     9001 gatataacag aagaagagtt tttaaatgta ataagacaac atgctattga tggagtagac
     9061 tttattacta tacatgcagg tcttacaaga agtgtttgcc aaaaaataaa aaatcatgag
     9121 agattgactc atatagtatc tcgtggaggt tctttattgt ttgcatggat ggaattaaat
     9181 aataaggaaa atcctattta tactaatttt gataaaatat tggatatatg tgaagaatat
     9241 gatgttacat tgagtttagg agatgcttgt agaccaggtt gtataaaaga ttctactgat
     9301 ggtgttcaaa ttcaagaact tgtagtatta ggtgaactta ctaaaagagc atgggaaaga
     9361 aatgtacaag taatgataga aggtcctgga cacatggcta ttgatgaaat agaagcaaat
     9421 gtagtattag aaaagagatt atgccatgga gcaccttttt atgttcttgg accattggtt
     9481 acagatatag caccaggtta tgaccatata acaagtgcaa taggaggagc tttagcatgt
     9541 gctaaaggag tagatttttt atgttatgta actccagcag aacatttaag attgcctaat
     9601 ttagatgata tgaaagaggg tataatagct gcaaagatag ctgctcatgc aggagatata
     9661 gctaaaaatg ttaaaggtgc tcgtgaatgg gataataaga tgagtaaagc tagagctgat
     9721 ttagactggt gtgagatgtt tagacttgca atagaccctg aaaaagctaa gagatacaga
     9781 gatgaatcaa ctcctactca tgaggatagt tgtactatgt gtggaaaaat gtgctcaatg
     9841 agaacagtta agaaaatatt aaacaatgag gaacttaatc taatataatt tttggctagt
     9901 atgactggaa taaaaagaag ctgaaaagag gaggtgattt agtgaaggtt aatgggaagg
     9961 aaattgaatt tgaaaaagat ttgactgtta tagatttatt aaataaatat aatttaaaaa
    10021 gtgatagagt tgtagttgaa gtgaatttag agataattga agaatctaac tacaatacat
    10081 atgtattaaa agacgaggac attgtagagc ttattagctt tattggaggt ggttaaaata
    10141 aaaatatttg taaatgaatt attaacagat gttgaggaaa atataactgc ttataaagtt
    10201 agagatagct ataacaataa atgtgatgta ttggtgttaa atggatatcc aattaaaaca
    10261 gatatgcccc tttgtgaaaa tgataaatta acacttatac aaaaaggagt taagccatca
    10321 cttgatgaat tggaaagctt gttgattgct agacatactc caaatataca taataaatta
    10381 aaaaaaggga aagttgctat attgggattg gggggacttg gttccaatat agctatctct
    10441 ttagctagaa taggagtggg agaactttta cttatagatt atgatgttgt agaaccatct
    10501 aatttaaata gacagcagta ttttatagat gatataggaa aactaaaaac tgatgccatg
    10561 atagaaaata taaaaaaaat aaatccattt ataaaattaa ataaacgaga tattttttta
    10621 aacaagaaca atatggatac tattaaggat tcagatttaa taattgaggc ctttgatgat
    10681 gcttcatgta aagcacaggt atgtaattat gtattaataa atttaaaaga taaatattta
    10741 atagcatcat ctggtatggc aggttactat gattcaaata taataactac taagaagata
    10801 aaagacaggt tttatatatg tggggatttt gtgaatgaag ctaaatttgg tgaagggctt
    10861 atggctccaa gggttgccat ctgcgcaaat catatggcaa atttggcaac gcaaatactt
    10921 atagatatgt aaatattaaa attagatata aaaaagattg aatattaaaa aaatttaacg
    10981 tggagggaaa taatatggat aaattgatac ttggtggaca tgaatttaat agcagattat
    11041 tggttggaac aggtaaatat ggttcaaata atatattgcc tgaggtaata aaagaaagtg
    11101 gaagtgaaat aataacaatg gcacttagaa gagtggattt agacaataaa caagaaaaca
    11161 tattaacgta tattccaaaa gagatgacaa tacttccaaa tacatcagga gctactaatg
    11221 cagaagaagc tgtaagaata gctagaatat ctagaaaaat gggatgtgga gattttataa
    11281 aaatagaagt aatatcagat actagatatt tacttcctga taatgaagaa actataaaag
    11341 ctacaaagat acttgctgat gaggggttta tagttcttcc atatatgaca ccagaccttt
    11401 atgctggaag aagacttata gaagctaatg cagcggcagt tatgccactt ggagctccaa
    11461 ttggttcaaa tagaggtctt caaatgaaag aaatgataag aataatgata gatgagttgg
    11521 atataccaat aatagtagat gcaggtattg gaaaaccatc tcaagctatg gaggctatgg
    11581 aaatgggagc tgatgcagta cttgtcaaca ctgctatagc ttcagcagga gaccctgtac
    11641 aaatggctag agcatttaaa ttagctgtag aaggtggaag agaagcatat attgcaaaaa
    11701 caggaaatgt gtcagaattt gcaaatgcat cttctccttt aacaggtttt ttaggcaatt
    11761 tatagaaagt ggtgattggt gatgagtttt tatgatgtaa tagaaaaata tagggatttt
    11821 gattttgatg gatatttaaa taatgttact gataatgatg ttttaagaag tttatcaaaa
    11881 gacaagcttg aagattttga tatattaaat ttgctttcta aaactgcagt aaaacattta
    11941 gaagatatgg cacaaaaagc acataaatta agtgttcaat actttggtaa aacagtatgt
    12001 ttatatacac caatgtatat agcaaattat tgtgttaatc aatgtgttta ttgtagctat
    12061 aatataaaga gtggaataaa aagaaaaaaa ttaactatgg atgaaattag agaagaggga
    12121 gaagcaattt caaaagaagg tttcaaacat ctacttgttt tgactggaga aagtagtttt
    12181 cactcaagtg tagaatatat aggtgaggct atagaaatat taagagagaa atttccctct
    12241 ataggtatag aagtttatcc aatggaagta gaagaatata agtacattgt agataaaggt
    12301 gttgaaggat taacagtata tcaagaaact tatgacgagg aaatatataa gagagtacat
    12361 ataaaaggcc caaaatcaaa ttataaattt agactagatg ctcctgaaag aggggcaaaa
    12421 gcaggtatga ggactttatc aataggtgca cttttagggc taaatgattt tagaaaagaa
    12481 acttttttta caatattgca tgggaaatac ttaaaaacga aatatcctca tattgagctt
    12541 tcatattcaa caccaagaat gagacctttt aaagggtgtt ttgaggaatt ggtagatata
    12601 agtgatactg atttagttca agctatggta tgtatgagat tatttgaccc tcatgcagct
    12661 ataaacatat ccactagaga aaatttagaa atgagaagtc atataatacc tttaggagta
    12721 actaaattga gtgcaggtgt atctactgat gtaggaggtc attctcaaga tgaacatgat
    12781 actgctcaat ttaagataaa tgatgaaagt actgttaaag atgtggagaa aatgctaaac
    12841 tcaattggtt atcagcatgt gtttaaagat tgggaaagat tttaaatgta tctgataaca
    12901 aacagaaaat tatgtagtga ggaacgatat ttagaagtta ttaaagagtc tatattgagt
    12961 ggtgtagaaa atataataat aagagaaaag gatttagaat atcaagagct aagaaaatta
    13021 tatatgaaaa taaaaactaa aatcaattgt atagattttc aagagcaaat atcagatgaa
    13081 agtttgaaaa ctaatataaa tcaaaaagag tttagaaata aattcaaggt taattttata
    13141 ataaatagta acattgaatt ttttgaaaaa gtggattgtc aaggtatcca tcttcctttt
    13201 aaattattct tgaatttaat tgaaaataag tataatttta atgaaaataa aatactagga
    13261 ttatctttac ataaagtaga ggaagtagat tatttagaaa aattaataag aaatcaaaat
    13321 ataaaaatag attacataac attatcacat atatatgaaa ctaaatgtaa agaaggatta
    13381 aatccaaaag gaattgagct tttaaaagaa gctaaaaaga ttactgatat taaaataatt
    13441 gcacttgggg gaattttacc atctaatgtc aaggaaacat taaaatactg tgatgatttt
    13501 gctataatgt caactataat gaggtctaaa gatattaaaa aaaccatatc aaattataat
    13561 gaaaagttga attagtaaat atagtatatt gaatttttaa cttaaataaa aataaaaaag
    13621 ttatctttta aattttaaaa ggtagctttt ttattttata tctttgctaa taaaaaattg
    13681 taaaaaggga aaactttagt cagagagatt aaacaatatc ctagaaagat ttaaaattta
    13741 gtaaagtatg gaggacatta taatgactga cagtaaaaag aaacgattta ttataccaca
    13801 cacttttaca ataatatttt tactaatagt cttgatggca atattgactt ggatagtacc
    13861 aagtggtgaa tttagtagag aagatgtaga aggtagaatg gtagttgtac cagggactta
    13921 tcaaagtgta gaaagtaatc ctcaaggatt tgcagatgtt tttaaagcac caatagaagg
    13981 ctttatcgat tcagctgaag ttgtaggttt tgttttaata gttggaggtg cttttggtat
    14041 tgtaaatagg actggagcta tagaagcagg aatagcttcg gttatctcta agtttaaggg
    14101 aaaagaactt cttataattc caatatcaat gattttattt ggacttggag gtacaacatt
    14161 tgggatggca gaagagacat taccatttta tatgatattt gttccactca tgacatcctt
    14221 gggatatgat tctctaacag caatagcaat tgtatttata ggagcagcag caggagcagc
    14281 cgcttcaaca ataaatccat tttcagttgg tattgcacag gcactagctc aacttgctcc
    14341 tggttcagga gtaacttaca gagtagttat ttttatagca tatatactaa taagtatagc
    14401 atttgttatg tactatgcta agaaggtaaa ggaaaatcct gaaagttcaa tagtatatga
    14461 aatagataaa aataataaat caatacttaa tcaaacttca acagaaataa aagaatttac
    14521 ttttaaagaa gtatctgttc ttgctatatt tggaataggg atggcaataa tgatttgggg
    14581 tgttttaagt cagggatggt acattccaga aatatcaatg tgttttgctg caataggtgt
    14641 gctttctgga ttggttggta gattatctca aattgaaatc tcagaaagtt ttatagatgg
    14701 agcaaaagat ttagcatctg cagctcttgc tatagctttg gctcgtggaa tcgtaataat
    14761 tgctcaaaat ggatttataa tagatactat attaaattcc gcagcagcct ttctaggtgg
    14821 gcttccaaag gttatatttg tatatttatc atttttcttg gaagctgcat tggcattttt
    14881 aattccatca tcttcaggtc ttgcttcact tacagtacct gtacttgctc ctcttggtga
    14941 tttggttaat atatcggctc aagtgatagt aaccgcttat caatttggtg tgggtcttac
    15001 aaactttata actccaactt caggagtact gatgggagca cttgcagtag cccatattcc
    15061 attttcaaaa tgggtaagat ttataatgcc tatcttgttg attttgacag ctgtatctct
    15121 agtcttttta acaataggta tttatatagg gttttaatta agtgtagttt tatttaagat
    15181 atttagtaat ggtataaaat gataatatgt tttcaataaa ttgccactaa tttaattagt
    15241 ggtaatttgt tttttgtatt taaaaataaa tgtatttact tgtattttga attaatatct
    15301 acatagacat tgttttaatt gttatatttt catttaatta agcattgatt tatcattttt
    15361 tatatttcat ataacaatgc aaaaaataat ttttatatag taaatgacaa aagtttacaa
    15421 taaatgtata ttttcttact cattccttaa aattactcat ttagtttaat aactctcatt
    15481 tcatatgtaa agttggtaaa aaaattaaaa ttaaaagtaa aaaactaaat aaattaatgt
    15541 taatttgaaa tatatatgaa ggtttttgga gaatacttac ttatgatagg aaaaaatgtt
    15601 aaaataatag tgtaatcaaa tataaaggac tgaaataatt aacagttatt aaatattagg
    15661 agtgtaaaaa tttatgggat taaaagaatt tgattttatt gaagagtcta ttgatatgtt
    15721 gaggacaatg tcaccaacat tagaaactat atcagatgaa atagaagagt attttgaaaa
    15781 tatcttggat gaaaagaatc aagaatatat aaatgtaacc tctagaataa aatcagaatc
    15841 aagtttaaga gaaaagataa ttagaaatag atatttaaaa aaatatggag aagcaagtaa
    15901 tcttattcat aatgtttctg acttaatagg cctaagaatt gagtgtagat ttattgagga
    15961 tgaaaacaaa atatatagac ttttgagaag atattttaat aaaacagatg acaaaataaa
    16021 ctattataat aaagagaata agaatattaa attaaaacta tctgagaggc aacccaataa
    16081 acaaaaaaat ggatttgaaa tatataaaat agatggagtt tttgcgtatt tagatagaga
    16141 agtaaaattt gaacttcaaa ttaaatctct tgtaaatgtg ttttggagtg aaatagaaca
    16201 taaaataatt tataaaaaca atacatattt acttgctgat aaatttataa aggatatgat
    16261 ggactctata aagaataatc ttactatgat agataatcag cttttaagta tttataaaaa
    16321 ctttcattca gggaagtctt ttaatatgaa agtaagtaaa aaagaaattg aaaaattatt
    16381 tgcgaaatta gtctatgacg ctttttctga aaagatgaat aaaagtatag gttttgttgt
    16441 agattttaaa aaaccatgtg agactatatt gaactattct tttaataaac aagatataag
    16501 tgatgaagtt ttaggtaatt ttatgcttga tgagtttgca agattaaatg aaattgtaaa
    16561 caaggatata gattttaatg agcaaataga atttgaaaga gaaccaacat ttgatgataa
    16621 gttttgtaaa gacctaggaa atcattttag aagtagatta aacacagaat ttccatggaa
    16681 cctatttttt agaatattat ttgaaataga gccatataac aataccaaag actttgaaaa
    16741 ctttgtagag tttataaaag aaaatgtgct actagaagaa aatagggagc aattattatg
    16801 ccaatttgaa gaagatagca aacttataat tgaagatata tatgattgta tatttaatag
    16861 tataagtgag attgatagtg ttgaaatttt atataactat aatcttgaaa aaataaactt
    16921 tacatcaagt gaaatcataa attgtatatg tagagaatat gagtgttatg gtgaatactt
    16981 agaagaaaaa gaaaagctta tgaatacatt taaagaaaag attattcaaa tctttgaata
    17041 ataggttaat gctgaagtgg cgctaatgcg ccatttttca aatatatatg aattaagtaa
    17101 aattattttg gaggaggcga tttgtataga gaaaacaggt gtaattttgg cagggggaag
    17161 aaattctaga atgggtagag ataaagcctt tttagagtta catgataagc tttttattga
    17221 aattgcaata gaagcattta aaaactttga tgagttgata ataatatcta ataatgaaga
    17281 attatattct aaatacgata ttaaagttta taatgatata gtaaaagatg ttggtccaat
    17341 tggaggaata tatactgctc ttgaatatgc aaagtatgat atagttacaa ttgcatgtga
    17401 tatgccatat ctaaataatc aaatagtcga aagaatagct aataaaatga atgataaaag
    17461 tgtgatttca gtgacaaatg gaaagttaca accattgtgt tctggatata aaaagagtat
    17521 aataaataaa gtatctttat gtataaggga aaatgattta aagctcagaa gctttataga
    17581 taaaatagat aaaagttata tatactttga tgaagaagat ttatttttaa atgtaaacac
    17641 tgttgatgag tatgaaaaat taactaaagt ttaattagct atggttgtaa aatagagtag
    17701 gatttatatg gaggagagtt tatgaattta cttaaatgcg attcttgggc agtaatatta
    17761 aataatagcg ataaagaaag taaagcatat aaaatattag atgaacttaa aaggaatatg
    17821 tacaaagttg ttgctataga tgaagagaaa aaacctattg aaggaataga tgtgtatgaa
    17881 tgtttaaaag atgttccaca caatattgat gttgtagcta ttattgataa acaatctaag
    17941 atggatgtga ttttagaaga agtagagctt ttggatatac aaaatatgtg gtttgaaaaa
    18001 ggaagcttta gtgaaatgat gattaaaaag actaaagatt taaaattaaa tatagaatat
    18061 aatttaagtt tatatgatga gttgactaga taaaattata tttcttaata ataaaaatat
    18121 aattagttaa taaaacatat tattttatag tatagattgg ttaataaaag tacagattgg
    18181 ttaatgaaac attaaattac aatatttgtt aaaaaaattg tgagttacat gatataatgt
    18241 attaaatagg atactaagga gttgatatat tatgttaata gtaactacag aaaaagtaga
    18301 aggtaaaaag atatcaaagg ttttaggatt agtgagagga agtacaataa gagcaaaaca
    18361 tgttggaaaa gatataggag caagttttaa gaatcttgtt ggaggagaac ttactggata
    18421 taatgaaatg ctcactgaag caagacaaat tgccataggt agaatggttg aagatgcaaa
    18481 agctaaaggt gcaaacgcag taatagcatt tagactgtct tcagcttcag ttatgcaagg
    18541 agcagcagaa atgcttgctt atggaacagc agttgtttta gaagatgata atagtattct
    18601 tgaaaaataa aatataaata gttaaaaaga ggcatctttt aaaaatttta tggatgcctt
    18661 tttttatttg aaaaaactaa tttgagaaga aattttaact attatgacaa taaaattagg
    18721 gtgatattat ataaataagt aaaattatat tatataatgt atggtataga ctttaaaaat
    18781 tttatttgta gagaaaaata tggacaatgt tataataatt atagaaatta aaagtactta
    18841 cattcattat gtatgtgaca tgtataatta gttttatatt taatacagtt ttgaagtaat
    18901 atcttcattt tgagaacgtt ggaataataa aaaatgtatg tattataggt gatataaata
    18961 aaaaataaat ataatatgtt atattgctga tagaaaagag aataaaagaa aataggtttt
    19021 attaaaagag atttttattt ataggactta atcgatgtat agaggggtga gaacatatgt
    19081 ttaatgttgc aattataaca cttagtgata aaggatatga aggaaaaaga gaagatataa
    19141 caggaaaaaa gttaactgaa tttgttgaaa atacaggagc ttataaagtt actgaatatg
    19201 tacttataaa agatgataag gaaatgctaa aggaaaatat tataagactt tgtaatagta
    19261 ataaaataga tttaattctt acaaatggag gtactggttt ttcaaaaaga gatataaccc
    19321 cagaagcaac taaggaagtg ttggaaaaag aaataccagg tctgagcgaa tatatgagaa
    19381 tgaaatccac agaaattact aaaaaggcca tactaagtag gggtgtgagt ggaataagaa
    19441 ataattctat aataataaat ttacctggaa gtcctaaagg tgcagttgaa aatctaagtt
    19501 ttataattga tgttttagac catggcatag aagttttaag aggagaagct acagaatgtg
    19561 ccactaaaaa aagtgaatag tatataatct agatagataa aaaatagaca aagaggtaga
    19621 taaagtatga ttgataagta tggaagaaaa atagactacc ttcgtatttc acttacagat
    19681 aagtgtaatt taaggtgtgt ctattgtatg ccaccagatg taaaatttga taaaaattat
    19741 ataaatgaaa atctaagctt tgaagactac aagtttatta taaaggctat ggctgaacaa
    19801 ggtataacaa aaattagatt tactggagga gaacctcttt tatatcctaa attaaatgag
    19861 cttataaaat ttacaaaaga agaatgtgga ataaataaca taggaatgac cactaatgct
    19921 attgggcttt ctgatagaat ctttgagctt gaaaaatgtg gcttaaataa agttaatata
    19981 agtttagatt ctttgaagga atataagtat aaatccgtaa caagaggagg aaacttaaaa
    20041 gatgtcttaa aatcgattga atcttgttta aacttaggta taaaagtaaa gttaaattgc
    20101 gttgctataa gtgggtttaa tgatgatgag cttaaagatt ttatgttttt aacagttaaa
    20161 tcaccaattg atgtaagatt tatagagctt atgccattag gtgaggctaa tagagtttat
    20221 aagaagggtt attttaatgt aaaagaaatg attgaaaaaa tatatggttt atataaagtc
    20281 aaaaatgatg aaaaaagtac agcagaatat tatatgttta aaggagctaa aggaagaata
    20341 ggaattataa ctccattaag ctgttcattt tgtaacactt gtaatcgtat tagacttact
    20401 tctagtggtg caataaaatt atgtctacat tcaaaagaag aaatagatat aaaaccattt
    20461 ttacataaac cattgttatt tagagaatct atgaaagata taataaaaca aaaaccagaa
    20521 aaacatcatt tactagaaaa taaatgtagt gatacaaata ggcgtatgta tgaaattgga
    20581 ggataagtaa tgttaacaca tataaatgaa aaagggtatg ccaagatggt ggacataagt
    20641 gaaaaagatg atactaaaag aacggccgta gctacagcta caattactct tagtaaggaa
    20701 gcattagaaa aagtaaaaaa tggtcagata aaaaaaggag acgtattgtc tgtagctcaa
    20761 gttgcaggta taatgggagc taaaaataca tcttcaaaca taccaatgtg ccatcagatt
    20821 aaattagttg gatgtgattt agaatttgat gtagatgatt ctatgtctgc tattaagata
    20881 tacgcaacct gtaaatctct tgggaaaact ggagtagaaa tggaagcttt aaatgcatgt
    20941 tctttagcag cacttacaat ttatgatatg tgtaaagcag ttgataaaaa aatggttata
    21001 agtaatatcc atcttgtaga gaagactggt ggaaaatcag gagattttaa gttcgagtaa
    21061 attaataata tattgggtta aaatttggag gaaatatggc gaaggtagtg tcaataaata
    21121 taagtgagaa aaaaggtgta attaaagttc ctgtacaatg tgcagagcta aaaataaatc
    21181 atgggatagt tggagatgca catgcaggaa actggcatcg tcagataagc ttacttggaa
    21241 aagaaagcat agataaaatg aaaatgaaag gctttgacca attaaaatat ggagattttg
    21301 ctgaaaatgt aactacagaa gggatagaag ttttttccct gcctgttggt acaaagttaa
    21361 gaatagggca atgtgaagtt gaagtaactc aaatagggaa aaaatgtcac aatggatgtg
    21421 aaattaagaa attgacaggt gactgtgtga tgccacgaga aggaattttt gtaaaggttc
    21481 taaaagaagg aattataaat gtgaatgatt caatagatgt aatctaatga agtgactatt
    21541 aaaatataat tataagtaaa tactattaag atttaaatat atattttaag agagaattaa
    21601 ttttaataaa atattttagt tataaattgg aatattggtt atataaagct atgaatgtaa
    21661 aatcatagct ttttgttttt gaattttaaa atataatatt ttatttatat gtattaaatt
    21721 atattttata taaaatcaat tgtttaaata tatattattt atactgtaaa tttatagata
    21781 ataaagtata aaaaaaatat attttgatga aatttaaaaa aatacattat ataaatatag
    21841 taatagtatg aaaatttaca tataaaatta aaaaatgcaa gtaattgact ttttaagttg
    21901 acaaaatatt gcatatatat tatgatataa atcataaatt aactacaaat caatattata
    21961 tattcgtaga agaagaaagt agcataaagg aattttacag agagttagat ttggctgaga
    22021 actaatattg gaatttatgt gaaaaggact ttggagaata ctattgtaat tatgttagaa
    22081 tagtggttta attcgcctta agaaaatgag tgagtaaaat taatttttac taataagagt
    22141 ggtaacacgg atttatattc gtctcttgga tttttccaag aggcgttttt tattataaac
    22201 ttatttcaga aaatttaaaa ctatatatta aatgaaaatt ataaaaatat gatagttaat
    22261 aaatttataa attagaaaat tgtaaaatat gctaaggtca gtagttttac gatttaaatg
    22321 gagggatgaa aatggaagaa gaaaaaatta actatgctct ggaacagatt ccagagaatc
    22381 aaaaacgtgg ttgggtagct atgttttcag ttttagtagc aataggtgtg gatttatcat
    22441 ctgttatttt aggcgctgag ttggcacaga gtatgccaat gaaacaagca atattgtcag
    22501 taattgttgg ttcatttttt tctgcaattt tgtatactac atgctctttg gttggttcat
    22561 ctacaagttt atctacatct atgataacaa agtatgtatt tggagaagct ggagcaaaaa
    22621 tattttctct agttataggg gtttctctat taggttggtt tggagttcaa gtaggtttct
    22681 ttgctcaaaa tgcacagata ataataaaag atatatttaa tttagatgta agtatgcaga
    22741 ttttaagcct aataggtggt ctcttgatga tgagtactgc aatttatgga tataaggcaa
    22801 tggagaagtt gagtgtgtat tcagtaccat ttttattagt tcttatgatg ctaactatat
    22861 tcttagcttt tagagcaaat ggaatttcag ttgatgataa tatgaaatct actatgacat
    22921 ttgcaggtgg agtttctctg tcaatgagta taataatagt tggtgcaatt gtatctcctg
    22981 atataagtcg ttgggctaaa agtagaagag actgtgctct ttcaagcttt ttgggaatac
    23041 aatttggaaa tgcttttatg ataatagttt cgatagtatt agtaaaatgt atgggaactt
    23101 cagatattat gagaattttt attacattag gaattgctat accaggtata atagtactta
    23161 cattagcaca atggactaca aatacaagta atgtatattc agcatcatta agtatagctc
    23221 ttgtacttaa gaaggctcct gagaaagtat taactatagt ccttggtatt atagcaacat
    23281 tattggctgt atttggaata tatgaaggtt ttataggatt tttaaattta cttggaattg
    23341 taattgcacc tgttggtggt gtatatacag cagaatatta catagtaaag caagaactta
    23401 aaggctttga taaaggagta ttatataaac caatagttaa acgttcaata gtgtcttgga
    23461 taattggtat attaataact tacctttcaa catatggatt tataactctt actactatag
    23521 cacctttaga tggttttata gcagggtttg tagtacaatc tattattgga aaagtgcttt
    23581 gttcaacaaa aaataagcaa gaaatagaca aagcagtata aagaaatttg tttataaagt
    23641 ataaaataaa ttagaaatat gaggagaaag taacatgaga tttttaacag aagaaagtgt
    23701 agataaaata gcagttggtg cagcagtgct tggaactgga ggtggaggtg acccatatgt
    23761 aggtaaattg gttgcaaaac aagccattaa aaagtatggt cctgtaaaag taataagttt
    23821 agatgaactt gatgatgatg ctttggtagt tccagtatct ggtatgggtt ctcctgtaat
    23881 tacaatagaa aaattacttt cagaagttga gttaactacc cctttagaaa taatggagaa
    23941 attattaaat agaaaagtgg atgttataat tcctatagaa ataggaggga taaactcact
    24001 tatgcctata gcagttgctg ctaaaaaagg acttcctatc ttagatgcag attctatggg
    24061 tagagctttc cctgaagctc aaatggtaac tttttatctt gaaggatatg aggcatctcc
    24121 tgccgtaatg tctgatgaaa aaggtaattc tgccatcctt tatcctgtgg atggaatttg
    24181 gtcagaaaga ttagctagaa ctttaactgt agaaatggga ggaagttctt ctattagtga
    24241 ctataattta agtggagctc aagttaaaaa agctgctata ggtaatactc ttacaatagc
    24301 tgaaactata ggagggtttt tacttgaaaa taaatctgat tcaaaaggtg ctgttgagaa
    24361 tatactaaaa gaattgcgtg gatacaagtt atttgaagga aaagtaattg atattaagag
    24421 agagttgaaa ggtggattta ctagaggaca tgcctttttt gctgggatta atgagtatga
    24481 tggagagtat tctatattat tccaaaatga gaatttaatt gccaaaaaag gtgatacacc
    24541 actttgtatt acaccagatt taatagcagt attagattta gaaactggat ttccaataac
    24601 tacagagaga ataaaatatg gctctcgtgt tatggtagta gcttttcctt gtaatgaaaa
    24661 gtggagaaca gaaaaaggaa ttgagacagt tggacctgga tactttggat atgatgtgga
    24721 atataaaaca gtagaagaat tacaaggaaa ataaaagttt aggaggaaat tttgatgtat
    24781 cgtgtaggaa ttgacgttgg aggaacaaat acagatgctt gtatactgga tggggaatta
    24841 aaagtaattc attcagttaa ggtggcaact acaaaagatg ttgaaactgg tgtttacaat
    24901 gccttaaaaa aagttataga tgaaagtaag attgaccatt cattaataaa atatgctatg
    24961 cttggtacaa ctcattgtac aaatgctatt gtagaaagaa aaagacttaa taatgtggct
    25021 atgataagaa taggaaagcc agccactact gccattcttc catttataga ttggccagaa
    25081 gacttaagag aaaaaataga attagattca atattggtat ctggtggata tgagtttgat
    25141 taaaaaatag ctacgcacac tcgtgacttt agtcatgagt tagtagctaa aatagtcagc
    25201 atatagggaa acctatatgt agagatagga ta
//
LOCUS       DAKBNN010000036        25179 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000036 DAKBNN010000000
VERSION     DAKBNN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.036"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(190..705)
                     /locus_tag="OUB02_001291"
     CDS             complement(190..705)
                     /locus_tag="OUB02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCT8885061.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1068..2138)
                     /gene="leuB"
                     /locus_tag="OUB02_001292"
     CDS             complement(1068..2138)
                     /gene="leuB"
                     /locus_tag="OUB02_001292"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCT8885062.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEELAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(2145..2636)
                     /gene="leuD"
                     /locus_tag="OUB02_001293"
     CDS             complement(2145..2636)
                     /gene="leuD"
                     /locus_tag="OUB02_001293"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCT8885063.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2651..3928)
                     /gene="leuC"
                     /locus_tag="OUB02_001294"
     CDS             complement(2651..3928)
                     /gene="leuC"
                     /locus_tag="OUB02_001294"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCT8885064.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3885..5627)
                     /gene="leuA"
                     /locus_tag="OUB02_001295"
     CDS             complement(3885..5627)
                     /gene="leuA"
                     /locus_tag="OUB02_001295"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCT8885065.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5751..5997)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6145..6591)
                     /locus_tag="OUB02_001296"
     CDS             complement(6145..6591)
                     /locus_tag="OUB02_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885066.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6612..7331)
                     /locus_tag="OUB02_001297"
     CDS             complement(6612..7331)
                     /locus_tag="OUB02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HCT8885067.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7449..8723)
                     /locus_tag="OUB02_001298"
     CDS             complement(7449..8723)
                     /locus_tag="OUB02_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCT8885068.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8923..10290)
                     /locus_tag="OUB02_001299"
     CDS             complement(8923..10290)
                     /locus_tag="OUB02_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8885069.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10540..11004)
                     /locus_tag="OUB02_001300"
     CDS             complement(10540..11004)
                     /locus_tag="OUB02_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HCT8885070.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(11009..11638)
                     /locus_tag="OUB02_001301"
     CDS             complement(11009..11638)
                     /locus_tag="OUB02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HCT8885071.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11672..12559)
                     /locus_tag="OUB02_001302"
     CDS             complement(11672..12559)
                     /locus_tag="OUB02_001302"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HCT8885072.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12588..13826)
                     /locus_tag="OUB02_001303"
     CDS             complement(12588..13826)
                     /locus_tag="OUB02_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885073.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14210..14734)
                     /locus_tag="OUB02_001304"
                     /pseudo
     CDS             complement(14210..14734)
                     /locus_tag="OUB02_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(14920..15306)
                     /locus_tag="OUB02_001305"
     CDS             complement(14920..15306)
                     /locus_tag="OUB02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8885074.1"
                     /translation="MLNKKLPDSEFKIMKYIWNTGYKTVISKDVADEMEKIYKWKQTT
                     TLTLLLRLTKRGFLASQKIGKHTHYTILIKEKEYLKSETKKLFGGLHNNPLSQLISKL
                     HDKEEVSIDKIDALEEWLRSLQEDEE"
     gene            complement(15346..15627)
                     /locus_tag="OUB02_001306"
     CDS             complement(15346..15627)
                     /locus_tag="OUB02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8885075.1"
                     /translation="MKEKYKWRKSTTEILLGRLVKMRILKKKRAGFQLNYEVLVTKKE
                     YLDVIKEEKDVSKYDNFFTQVFTTIHKKEEKTEKQIKVFIESMQKLGKK"
     regulatory      15943..16040
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16095..16271
                     /locus_tag="OUB02_001307"
     CDS             16095..16271
                     /locus_tag="OUB02_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885076.1"
                     /translation="MFKNKMDKCTHMLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(16476..16619)
                     /locus_tag="OUB02_001308"
     CDS             complement(16476..16619)
                     /locus_tag="OUB02_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885077.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     gene            16885..17073
                     /locus_tag="OUB02_001309"
     CDS             16885..17073
                     /locus_tag="OUB02_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885078.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            complement(17483..18049)
                     /locus_tag="OUB02_001310"
     CDS             complement(17483..18049)
                     /locus_tag="OUB02_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885079.1"
                     /translation="MLGAKKEKEFNLLSFNEKKIEKHKCKNYHNDSSKDKYKYSQSEF
                     IAATLKEADDMNEEERRILEAEKRILETEKRVHQDNKEVKESIDKQFNDIKGIFKEYK
                     NDLDKDRIELKNDLARERAELKSDLKEAINEHKKVTEKDVSEIKNSMKGIEDRIDSTN
                     KWIIGLCITTIIGIATMAITIGISIWPK"
     gene            complement(18051..18542)
                     /locus_tag="OUB02_001311"
     CDS             complement(18051..18542)
                     /locus_tag="OUB02_001311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885080.1"
                     /translation="MAYENGTDFIDYFPDCIEKYKEDNKIFEKAILLLNLYMQKPTWP
                     HCVNEYKSQAEKILGEDPEFIGKYGIQLRKFYDEDENAKVSKEFKNDLYNFSKSTYDI
                     LDNVYFSKSHPFNLSSIYSTSRDDDFIIKIFRYDNNFLELEMSKREVESLINYLSDLL
                     KEE"
     gene            complement(18792..19589)
                     /locus_tag="OUB02_001312"
     CDS             complement(18792..19589)
                     /locus_tag="OUB02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8885081.1"
                     /translation="MKIAIVPGHTLGGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSSNYLAEQCQIANKQDVDLAVQIHFNANKITLNPMGTETIYKTNNGKVY
                     AERVNEKLATVFENRGAKSDVRGLYWLRHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIKDYI
                     PGQTENLYVVGGGACNKISSITKEHYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(19589..19846)
                     /locus_tag="OUB02_001313"
     CDS             complement(19589..19846)
                     /locus_tag="OUB02_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCT8885082.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIAFSVWMLGLNPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(19866..20096)
                     /locus_tag="OUB02_001314"
     CDS             complement(19866..20096)
                     /locus_tag="OUB02_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCT8885083.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            complement(20128..20265)
                     /locus_tag="OUB02_001315"
     CDS             complement(20128..20265)
                     /locus_tag="OUB02_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCT8885084.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITASEYKEIVGEDYI
                     A"
     gene            complement(20266..20565)
                     /locus_tag="OUB02_001316"
     CDS             complement(20266..20565)
                     /locus_tag="OUB02_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885085.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIESLPT
                     QKYAENKEAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(20562..21794)
                     /locus_tag="OUB02_001317"
     CDS             complement(20562..21794)
                     /locus_tag="OUB02_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885086.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEDALKEVFQS
                     GVDAKNNVVTALNSKGAEVTTRDTWEEIKNKIDIKEGRLDLRETTLSNNYSYLVTNGA
                     IKYIERCSGNFKTFEYEEPYFYVIKETHLIKINAIDETVVFDITLANANFSCICITQE
                     YLFISDNTKLYKINKSTGSEVQSIEGAYYKLCTYGEFIYGVYGDETSSTLHKIRISDM
                     YIMLTKNLVSNGIYNFKGGKFVCNKNAIYATTEHSNSSSITTCHLSKINFDFAIAKDF
                     RIGGYLHMKNIKFLNDFVIVSDAERGIEIDSNKKSALVKYDANLNLIVYSDDSKYDNF
                     DIYNGYIYAIYSLSSSPFVKISLNTLKHIDSYRKLTETYPNAGMFVINDIVFFISSGI
                     FRNILSKKVYSDEKGESL"
     gene            complement(21828..22010)
                     /locus_tag="OUB02_001318"
     CDS             complement(21828..22010)
                     /locus_tag="OUB02_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885087.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNTEYRKAVED
                     YIIKQSGVVEGIEPTA"
     gene            complement(22010..22303)
                     /locus_tag="OUB02_001319"
     CDS             complement(22010..22303)
                     /locus_tag="OUB02_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885088.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGN
                     IDYSKQYIESINPITKEPILKDIPIYLSEEEKRIQELENQLLIAENEKVGGLL"
     gene            complement(22317..23795)
                     /locus_tag="OUB02_001320"
     CDS             complement(22317..23795)
                     /locus_tag="OUB02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885089.1"
                     /translation="MIDFSYETSKRKRKGIYSEQDIFESSHVFYPAGRGRAEAVFELS
                     SVKNISVTRNLNLSKLSSVINGENGVLENLTMEQKLEVLFIDTPKIEIYMDANISKGC
                     EISYRIFDDFASSYPFTVTEKINNEVVSTKVSSKDGKYTTILTDEQILKLEIGSKNTF
                     LIEISTEGGVVTSKQATFTRINNKPTILVNSYNSNSAKFTASDLDNNLSKIEWYLDDV
                     LKETITTDLTAEKTINYELTNNAIHTLKIVATDTENATVEKVLSISKEIMPLQSDATL
                     QDISTKLVEIGEGFKNGKTSIINTLALKNIEANLNNTLVELSEKIKASFDSGDASLQD
                     LMNQLTQANNTITQLNTKYKVASGTTSVIQSSQHVNLYGGLYGATYIRQPYHWININN
                     LGFVPNIFIAENETVSTGSVIEKNIIIATCNMSWSTNKRDFIANLQLTRGKMDDQDFN
                     CHAGFYINNEQDAYINSNGINVPASRTSLPIYGFSWYAIKFK"
     gene            complement(23812..24837)
                     /locus_tag="OUB02_001321"
     CDS             complement(23812..24837)
                     /locus_tag="OUB02_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT8885090.1"
                     /translation="MATDKSYYTILTDIGKAKIANASIVGEKVDFARIQLGDGGGNEY
                     NPTEEQTALKNVVWEGKVGNVKTDESMTNCLILESLIPASAGGFVVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IGTVNTKIDTTKAELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNTIEIRSETRS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEISKVAEQLTGINSNIISSI
                     NNNLIPM"
     gene            complement(24853..>25179)
                     /locus_tag="OUB02_001322"
     CDS             complement(24853..>25179)
                     /locus_tag="OUB02_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885091.1"
                     /translation="WLDSIVGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSDI
                     KPCNMTLDYTRVLVSKDNSVYFGATTITGHEITIYPWSPSDIETQGEINILSGNGFGY
                     QEVTIY"
BASE COUNT         8062 a         4421 c         2586 g        10110 t
ORIGIN      
        1 ttattctctc aaaaaaaata tactttttat ctatataaat taattttttt agtatgtaaa
       61 tttgacgcta ttacgataaa tctttttagt ataactaatt tatttttatc ttgtattttt
      121 attgtatttt taaattttat tttttctaaa tataaaattt atgttttaaa atgtaaaatt
      181 tttatttaat caaagttcaa tatattccac tttttctttc gattcttcaa aaaaccattc
      241 ttcattagaa aaatcacctt ctgcaatttg tccaataggc attttcatat actttccagt
      301 tgctgtcact gctatatctc catttggtag aattatttct cctgtacctt caaataattt
      361 tctgttttct ttagttattc ttccaacaac ttttatagta gcatctgttg gtactggttt
      421 cttatatttc aactctaaag atactgttac tccccatatt tggtcatcat ttatacttac
      481 agctcttcca atagtctcat ccaatattgc agcagacata cctccatgaa ctctcccagg
      541 ataactttga tgccaatcct ttgtattaca aattgataca agctctccat tttcaagttc
      601 ataaaaatct gctttaagtc ctagctcatt ttgtgttcca caaactaaac aagatttact
      661 actattttgc ttcttcaaaa ctttatactt tgccaaaaaa tccatctcct aaccgacatt
      721 agtagtcgct taatattaaa taattctaaa atatattata taatattaat aaaatccctg
      781 caaacttcta ataataaaaa taggctactt caatatgata agtttaattt aaagtattat
      841 tttccaaaaa ataactttat caaaatagaa actcaaagta gatataagcc tctttttcgt
      901 tattaaaata gtaaaaatat attttactta atataaaaaa gagtgtctca ttaactaata
      961 aagtttattt tgagacaccc ctcataaaac aaattatact acaatagatt tttactaatt
     1021 ctacaagtta tatattcaaa ttatattatt caaattaaac tataattcta aatttcattt
     1081 actattaatt cgcccattgc tcttgttcct acaacatttc ccacaccatt atagatatca
     1141 atagttctat aacctttatt gagtacactt tgtacagcat cttcaataca cttagcttct
     1201 tcttctaagt taaaactatg tcttaacatc atgcttacag atagaatagt cgcaattgga
     1261 tttacaatat cttttccagc tatatcaggt gcacttccat gtattggttc atacatacca
     1321 aaactatctt ctctaagtga tgctgatggt aacataccta tagaacccgt aatcatagat
     1381 gcttcatcag atattatatc tccaaatata ttattagtca ctattacatc aaattggcta
     1441 gggtctttta caacttgcat agcagcatta tcaacataca tatgtgacag ttctacatct
     1501 tcaaactctt tagctaattc ttccattata cttctccaaa gtcttgaact ctcaagcaca
     1561 tttgccttat ctacacttat aagtttttta tttctatttc tggctatttc aaaagctctc
     1621 ttaccaattc ttcttatttc attttcgtta tatatttcta catcataagc attttcgata
     1681 ccatcaatga tttttcttcc acgttctcca aaataaatgc cccctgttaa ttcccttaca
     1741 acaacaaaat caatgccttt ctcaactata tccgttctaa gtggtgaagc ttcttttata
     1801 ctttcataca ttgtagctgg tcttaaattt gcaaataaac ccaatgactc tcttaatttt
     1861 aaaagaccag actctggtct tttatctccc ttacatttat cccactttgg tcctccaaca
     1921 gcaccaagca atacagcttc gttttttcga caagtttcaa cagttttatt aggtagaggt
     1981 tctccagttt catcaatcgc gcatcctcca gctaatacat attcacaatc gaatctatga
     2041 ttaaatttac aacaaatttt atttagaacc tttatacctt catctataat ttccggtcca
     2101 acaccatcac cttttatgac tgctatatta caattcatac tccactaccc cctatttgct
     2161 tttattgagt taattaagcc ttcatttaat ataatatttt gcataaactc aggaaatggt
     2221 tctgctttat attcttttcc ttttgttata ttttttatca cacctgtaga aaaatctact
     2281 tctatattat ccccttcatc tatgttattg gcagcttctt cacattcaag tataggaagc
     2341 cctatattta tagagtttct aaaaaatatt ctagcaaaag tagaagcaat cacacaggat
     2401 actccacttt cttttattac aattggtgca tgttctcttg atgaaccaca accaaagttt
     2461 ttagttgcaa ctataatatc tccttttttt accttagaga taaatttatc atcaatatcc
     2521 tccatacaat gcgttgctag ttctttataa tcagctatat ttaaatatct agctggtatt
     2581 attacatctg tatctatatt gtcaccgaat ttaaaaacac ttccattggc aatcataaat
     2641 tttacctccc ttaaattatt tctgatggtt ttgatatttt tccagttata gctgatgctg
     2701 ctgctacagc tgggcttgca agatacacct ctgaatcaac atgccccatt cttcctacga
     2761 aattcctatt tgttgttgaa atggattttt ctcctgctgc aagtataccc atatgtcccc
     2821 ctagacatgg accacaagtt ggtgtagaaa ctgctgctcc tgcctctata aaagtggtaa
     2881 tatacccttt ttctatagct tcaagatata ctttttgagt tccaggaaat atcataactc
     2941 taactccatc tgctactttt ttgcctttca ttatctcagc tactacttca aggtctgata
     3001 ttcttccatt tgtacaagag cctattacaa cttggtctat ctttatatcc ttatctatct
     3061 catctactgt tctagtattt tcaggaagat gaggaaaggc tactgtttct tttagtttgg
     3121 ctaaatctat ttcgtatact tcatcataga ctgcatcttc atctgcttca tatatattta
     3181 catcttttgt catagttgaa cacttatgtg aatttatata ttccatagtc ttatcgtcta
     3241 ctggaaaaat tccatttttt gctcctgctt ctattgccat attacaaata gtaaatctat
     3301 catccataga taagtattct actccatctc cacaaaattc cattgacttg taaagtgctc
     3361 catcaacacc tatctcccct attatatgaa gtattatatc tttaccactt atccatttat
     3421 ttggtttatt tttcaaaaca aattttattg ctccaggaac cttaaaccaa cattttcctg
     3481 tagccatacc tgctcccata tcagttgaac ctacaccagt agaaaatgct cctaatgctc
     3541 catatgtgca agtatgagag tcagcaccta taattacatc tccagcagtt actatacctt
     3601 tttcaggaag aagaacatgc tctattccca tatctcctac atcaaaaaaa tttacaactc
     3661 cttttgattt tgcgaaactt cttgtaaatt tacattgttc tgcactcttt atatctttat
     3721 taggtgtaaa gtggtctaaa accatcacta ctttagtctt atcataaaca tcttcaattc
     3781 ctaatttttc aaattctcta atggctactg gtgatgttat atcattacct aaaactacat
     3841 ctaaatctgc ttcgattaat tgccctttct taacttcatt taatttagca tgtttggcta
     3901 atattttctg cgtcatagtc atccccattt tttatttcct cctcaatata acttttattt
     3961 attgcattca taagtgcatt aatagctgct gtatttatgt tttccgatat acttacacca
     4021 aaatagactt cattattgtg ctctattcct acataagcta cccctcttga atgtgaccct
     4081 tcctctaatg catgttccca gtaatatcta aatttataat ctttcatgcc acattgcttt
     4141 agtgcattat taaatgaatc tacaggtcca tttcctatac caacaatcct ttgttcattt
     4201 cccatataac ttattcttgc agtcatctca atcatgttag tatcattaga atcatcattt
     4261 tcatagttta attcatccat tgattttatt ttatacttct taactttata agggctctca
     4321 acttcaatat attctttctt aaacaaatta aatattgctt cactactaag ttcttcccca
     4381 attctatcag attccatttt gactacattt ccaaaatcag aatgcatatt cttaggtatc
     4441 ataaatccat aatctgtttc cattatataa acagctcctc cttttccaga ttggctattt
     4501 atccttataa tttctttgta ttctcttcca atatcatgag ggtctattgg taaatatgga
     4561 acttcccagt aatcattatc tctgtttttc attgctatca tgccttttct aatagcatct
     4621 tgatgtgaac ctgaaaaagc acaatgtaca agattacctg catatggatg tctatcatgt
     4681 accataagtt ttgtacagtc ttcatatata cctattatct tatctatatt gctaaaatct
     4741 agctctgggt ctattccttg tgtataaaga ttaagtccca catttactat atccatattc
     4801 ccagttcttt caccatttcc aaatagtgtt ccttcaagtc tatcggcacc agccaataat
     4861 ccaagttcac ttgcagccgt acaagttcct ctatcattgt gagtatgcaa acttagtata
     4921 atagaatccc tacaacttat atttttacaa aaccattcta tttggtctgc atatacgttt
     4981 ggtgttgcca tttcaactgt tgatggaaga tttattatta cttttttttg aggagttggt
     5041 ttccaaacat ctatcactgc ctcacatatt tcaagtgcat aatccatctc tgttcctgta
     5101 aaactctctg gtgaatattc aaaagtaaat tctgtttctg gatatttttt tacttcttca
     5161 ttaaataatt ttgctccttc aactgctata tctattattt cttgtttact catattaaaa
     5221 accacatctc tttgaagaac agatgtagag ttatataaat gtataattgc ttttttacat
     5281 ccccttattg attcaaatgt tttttctata agatgagccc tagattgtgt aagtacttgt
     5341 atagttacat catctggaat catattctca tctattaatt ttcttaaaaa tgtatactct
     5401 gtatttgatg ctgatggaaa tcctacctct atttctttaa atcctgtatc aataagcatt
     5461 ttgaacattc tgactttttc atttacactc ataggtgttg gcagtgactg atttccgtct
     5521 cttaaatcga cactacacca aattggagcc ttaaatattt cattatctgg ccatttacga
     5581 tttactattt gcattttatc atatttttta tactttccac acttcatgtt atttccccct
     5641 ttttctgttt tttgtattca gtatacctca tatcgacgca atcctgcaca attcataagg
     5701 ataaatgtca attttatcat tagaataaaa aaaacctcat ctctaataaa attagagacg
     5761 aggttttgct cgcggtacca ctctatttag ctttctaaaa agctccctta gtaatagcca
     5821 atacaataat tataatgact atatttccta taacgtggaa ttactccgat tattctaaga
     5881 tttaaatctt agaaaacagc tcctagacaa gttcagaact atcaactact aaatcacacc
     5941 atcctttagc tctctgaaag tttcattgtt cttactactt ctattcatca cttttttaat
     6001 catttaaatt ttctaaatat tttgatttaa tagttattat atacacatta aaatgtattg
     6061 tcaacacaaa aaataattat ctcaattttt tatttttgat aagcaattct ataaaacatg
     6121 ctaactattg aaaacagtat gatattatgc cttaaacttt ttcatctttt catctatgat
     6181 attcaataat tttttttcat cttccaaact caatgccctt aatctaattt tataattaga
     6241 ttcgtcagtg tatatattta atatattttt tactacttct attttttcta ttatcttata
     6301 attaaacttt tgactttgtt tagattttat actgaatata cattgattat ttattaatat
     6361 tgaatgatta tcgagagtaa tatatacttt tccctcatat ttatttgaaa aatcacgacc
     6421 ttgtttaaat agaaaaactg ctgttattat acataaaata tataatattg tcgttgaata
     6481 tataaaaatt gtagctaaaa ttataagtgc taaaaccata tatatagtca ttcctttttt
     6541 aaaataacca ttgtcataat aattcatata gataaactta tttttactca aattttttca
     6601 ctcccaagat tctaaaaatt tatatcaaaa aattctataa tttcatcttc atgataattt
     6661 atgactaatt catctggaaa atctactgct tctaaaactt tttctgcatt ttcaaattct
     6721 cctatagaat aacatatatg agagtcagta ccaagaatta ctcttgcacc atatgtttta
     6781 cacaatgtca acatatgact tacattttcc caagtacccg ttctatgaga atttgaactt
     6841 aatgatgaat tatttatttc taaaaggata tttttatctt tagctttttt tactataggt
     6901 tcataatcta aaggatacct actgtcatct ggatgtccaa ttatttttac cttaggttta
     6961 tccataactt ttaaaatagc atttgtattt tcttcttttg taccagaagc tatacaaggt
     7021 ctatgcaaac ttgcgattat ataatccaaa tgttgcaaca tatcttcttg aacatctaaa
     7081 ttaccattat aatccattat atttccttct attcctctaa gaatctttac gccttgcact
     7141 tcacgtggaa gcaaatgcaa attataaaaa tagaatggat gtggtcctcc tggcatatta
     7201 ggtccatggt ctgatattcc taagtatttt atattcttct tactcgcctc ttcgacattt
     7261 tccttgaaag tactatgtgc atgtccactt attagagtgt gacaatgtac atccataata
     7321 gactttttca ttaaatttcc tcctaatatt ttacataaat tcgtgaaaat agctaaaggg
     7381 ctatttcaaa actaaagctg tcataataga cagtgtaacg ttttaaaaca gccccatttt
     7441 caattatttt acacttctaa atctttaaat ttatctttaa ataattctcc aatacttcct
     7501 aaagattcct ctacttctag gtattctgtt atatcctctt ctggctctct agttgcttct
     7561 tttattgaaa ccaacattct tttagtcttt ggtttaaaat caagaatctt aactttaacc
     7621 tcatctccaa tattaacaac attagtaact ttagccactc tagtatcact caattcagat
     7681 attggtaaat acgcatctac accttcttta atttgaacaa aagccccttt ttctattatt
     7741 ctaagaactt tagcattgac aacatcatca atttgtactt cttttgctat taaatcccaa
     7801 gggtcattat ttatgtcttt taatgccaag gcaatttttt tagtctcttt atttacatcc
     7861 aacacataaa cttctacttc ttgtccctct gaaacaacat cttcaacact ttcaactctt
     7921 gtccaagcta aattatttat atgtgctaat ccttcaatcc caccaatatc cacaaatgca
     7981 ccataaggca ttatctttgt caccttacca tctctttttt gtccaacttc aagagaatta
     8041 aataagtctt ctttagctgc tttgatacga gccttttctt cttctctttc ttgcttaatt
     8101 cttgctcttt cagcttcttt ttctgcctta atttgagctc ttctttcttc tctctcttta
     8161 tttatacgct cttgtaatac atctctatga gatgctacaa gtctattttt tctaacatcc
     8221 aattctttta tataaacttc taagttaaga ccaacaaatt tagatgtatc tgtaataaat
     8281 tttgtatcaa gttgagaaat tggtataaaa aatgtatatg tattgtaatt agcaaataca
     8341 cctttatcta tattcttttc aacatttaca gttattattc tatgttcatc ataagccttt
     8401 tgtaactcgg caaaatcatt tcttttgtca agttgtaatt tagataaagt tattgtacca
     8461 tctttttgag atacttttgt tattacagca gttatttcat cacctatgtg gtatatatct
     8521 tctatatatt gatttttttc tatgttcaat tcacttatag gtattattcc atcaaaccca
     8581 tagtctaatc ctaattgtac ttcatcattt ctcactgcag ttatttttcc agttgtcaat
     8641 tctccaactt tcactttgct gaatacttgt tcttgctggt caagtaattc ttgcattgtt
     8701 aaatcatttt ccattataaa catcctttcc tcttattgat tatcatatct gtaaatatta
     8761 taccattaat attgcaaata cttacatttt tcttactata atttttaaaa taaattcaac
     8821 ttcatatatt tatacattat agaaaatatc ttattaaatt aaaatacaaa ttctaatttt
     8881 tatctaataa aatattttca taaaaaattt atattgtata tcttattctt ttattgcttt
     8941 aggttttgat agaaaacatt tataaaatat aaagaaagaa attgctgtta tagtagcagc
     9001 tgttatatct gcaactggct ctgctaaaat tactgcaaaa agtttttcat taaagaatgt
     9061 tggaagtata aatattaaag gtattaaaag tacaatcttt ctaagtagag ctaaaaccaa
     9121 agatattgtt gcttttccaa gtgctaaaaa tgtctgttga caagctattt gaatcccaaa
     9181 cataaaagca ccagcaaaat atatttttat actccaacta gtcatactaa gtaactgcgg
     9241 attcttatta aatatactaa caaaaaatgc aggaaatatc attaataaaa gccataaaat
     9301 agtagaatat gtaaaacatg acaataaaca caacttaaat gtctttttta ctctatcaag
     9361 ttgacctgaa ccataattat aacttattat tggttgtgct ccttgtgtaa cccccatatt
     9421 aggaagcata agaatttgca ttatactgct cattatggcc attgaaccta ctgctaaatc
     9481 tcctccatac atttgtaatt tactatttaa ggctataagt actaaacttt ctgtagattg
     9541 cattataaat ggagagattc ccaagccaag cactggtaag atgatacttg cttttggtat
     9601 catatacttc ttttttattt taagtatact tttctttcca aagagaaaca taagcaccca
     9661 aatagcagaa acgccttgtg caattatagt agctaatgca gctcctttta cacccatatt
     9721 aaagccaaat ataaaaatcg ggtcaagtat tatgtttatt aaagcaccta tagcaactgt
     9781 catcattcca gttgtagcaa acccttgggt atttataaaa gtattcatac ccattgaaat
     9841 ctgaacaaac actgtaccta ttaaataaat atttaaatag tccattgaat atccaattgt
     9901 agattcactt gctccaaaca tccaaagtaa aggctctttt ccaatcttaa aacctatagt
     9961 caaaagtatt gctaaaatca ccagtacaga aaaactgtta ctcattattt tctctgcctc
    10021 atcattgttt tgctcaccca tttttatagc agctaatgga cctcctccca taccgattaa
    10081 ataactacat gcagttatta taattattat tggaaatgcc actccaacac cagccatagc
    10141 aacttctcca ttttccattc taccaataaa aatcctatcg actatgttgt ataaaacatt
    10201 tacaatttgt gcgataatag caggtgtagc aagtttaaat aataatttac ccacactttc
    10261 actacctaag ttagtgtttg aattttccat tttaaaacct cctcaatcca ataattaaaa
    10321 tgtatcacta aaattttatt ctatataaaa atttcatttt attagcagac aaatttagag
    10381 tactttccct acaagtaata ctatactata tttcttttgc catagtaaac attttagaac
    10441 taactgaagt taaatacttt atctcatttt ttatttatta ttaaaaattt ttcacatata
    10501 ctcataaaaa aactatccaa gtatattact tagatagttt tatattatat gtaaagaaag
    10561 agctgaaact ataacccctg ataacaaatt accaagaaat actcccataa aagcatcttt
    10621 tattctcatt tttaatatgg aagctatagc agatgcagtc catgttcctg tagttggcag
    10681 tggtatacct acaaatagta ttataccaat tatcgaaaca ctttttaatt tcttcattct
    10741 tgagtttatt tttctatcaa ttacatcagc aatcccttta aatagtttct tccctcttaa
    10801 aaattgcaat acatgtctga atgtcaatat caagggaatc gataccaatg tagaacctat
    10861 tacactaaat acataaaccc atataggatt taagcccatt gcaataccta taggaatggc
    10921 tcccctcaat tctgttacag gaaccataga taaaattaat atgtgtaaat atcccaaact
    10981 attccctcct attctaagtg acattaagtt atgaatttat ttgctctata acctcaccat
    11041 cctttttact aactaaaaat gcatttttag tcaatgaaag cattggtaaa tcactcttag
    11101 agtctgaata tgcatatgaa ttttcataat caatctctat cccaagttta cctaatacag
    11161 cctttattct ttttactttt tcaattccct tacagttttc tccaatgaat tgatttttat
    11221 atcttgaatt ttcataaaaa aactccgtgc ctataacctc gtctgcaaga ccataatact
    11281 taaaatattt catatatgca tatggagatg cagttatcat aattataaca ttatcttctt
    11341 ctttattttt gtaaataacc tttttcatag attcgttaat ttttttcatt atgcaatcat
    11401 caaaaaactt ttttaaatct tcttcactaa aatctactat tggataaaag attgcctctt
    11461 ttgccttctt caaaggtatc attctaatta aatacataaa tagaataaaa ataatgtatg
    11521 gcaactttat aatagtttta aaggttttat tttttaaaga gtaaataaaa aaatctatca
    11581 tgctatcagt atttataaca gtataatcta catcaagtaa taatacactt tttttcataa
    11641 gtatttcccc atcctatcac taaaaatatc actaaaaaat caatattatt aaacatgtaa
    11701 ttatccataa gattacgttt atttttaaag cattatcttt taggacaata tcagtaggat
    11761 tattttcatt tgtagtgtta attatataat tatatctaag tacaccataa actacaaata
    11821 ttgttgtaaa tatcatatgt ggcttttctg gattacttgt agaatacaat gcataacata
    11881 caattgtaga acttaatata actatcatca tttgatttag attttctatg tcataatctt
    11941 ccaatatttt tctatgttca actgcatcat ctcttaaagt ttctatttct tttttacgtt
    12001 ttccaagagc taagtacaga gataagaaaa atgtacataa aattatccaa tttgataaac
    12061 taacacttat ggctacacat cctgcataaa ccctaagtat aaaaccaaga gatattgaaa
    12121 acacatctaa tagtattata ttctttagtt taagagaata taatatattg tttatcaaat
    12181 agagactgag aattatagaa agattaaaac taacaactgt tgataaacag actgaaatta
    12241 aagctaatac tacaaatagt atattagcac tttttttgct tattcttcca cttgccactg
    12301 gtctattaca ttttttagga tgttttttat ctttatccat gtctatagca tcattcagtg
    12361 catatactga tgatgacatt agacaaaaac acacaaacgc aataaacgta tttaaaaata
    12421 aatttatatt tagtatgttg tttgaaaaaa ttatagctga aaatacaaag aaattcttta
    12481 tatattgctt tattctcatt aactttatat aatctgaaat tttagtaaga agccttatac
    12541 ttgtattggt ctgtttcata aacttactac cctttcaata ttatttttta aacaggatat
    12601 gattttatcc ctatgtttaa aatttctata aatccattga cattactgat tactatagta
    12661 accaataaca gcgatagaaa tatattatac atattctttt cattttttct aagcgctttc
    12721 gtaaagtaag ccaataaaat tataactgca aaactaaaat ttatagccat aatacttgaa
    12781 ttgactagat tatatcctac tataccatgt aaagttagat tgaatataat tattcccata
    12841 catagcttag gaactctttt ttctgaattt tcaataactg ataaaacaca aaaacataaa
    12901 gctaataatg taagaattac tggtattgta gcaacttctc cattttgcaa aaataaccct
    12961 tttttaatac ttgctccagg aaaataaaaa accgaataca ataatctctc tataatcatt
    13021 gcaatctttt ccaaaattgg ttctccactt ataaatttcg tatcatactg tattacattt
    13081 ccaaggttca taaaactctc ataagacttt gttgaaacta cacaaatata tgccattagg
    13141 ataaatgtaa ttaaatgttt taacattttt cctattttta atttaaactc aaatatcata
    13201 ttaaaaaaat acaaatatat attactaata gttattccaa atagaggaat agctgctaat
    13261 tctactaaat aagaattttt tcctttcatc ttatttgcaa taactatgaa aaatattaat
    13321 gaaaattggc tatatataaa tctctctaca tttaaagtca taaataactg tggaaaagaa
    13381 aatatcatta tcatagtaag taaaattaaa gttaatctct gcatttttat ctttttaata
    13441 tgattaaaga ctaaagttat tgacatagca ctagttatta tttgtagaca aattagtcca
    13501 aacccatagg cactattaaa atctgttgtc ccaggatttg ctattgaagt acaaaatagg
    13561 tatattggat atgcaaaaat tgatataatt gctgaaaaca atatgtgctt actattgtct
    13621 tgacttatag caaatacatt taaattgaat aaaactcctg tatctgtatc taacaataca
    13681 tcataagtat tgtttaaatc ataaaatata gtagtcttag aaaaaaatat cttagttatt
    13741 attaagtaag ttattacaac taatataaac aaaaacatct cgtttttata ttttaatatt
    13801 ttactcatca atatatcacc atccattcat tatataggat ttgctgacac ctacaattta
    13861 ttccttatat ttaatagcca tcttcataat gttcttactt gttttattgt aattgaataa
    13921 ctctttattt acaaccacaa tatttacttt attgatgtta taatcagtca agtattgttt
    13981 atataacata gattagtgtt gtataaaatg agaatttgta agcaatatta atgtaaaata
    14041 tttgaaaaac tttatataaa aatatacaat gttgcttatg gaattaaact ataacaaatt
    14101 aaagcataat ttaaggagac aaaattataa tctgtctcct taaatattaa aactatattt
    14161 taaaacatta ctaaatacta tacaaatttc aaatatagta taaaattaat tagactttaa
    14221 cactattagc tccatcttct tcccattttt taatttcttt taatctatct attataggta
    14281 aatgctgagt acattcaact tcacattttc cacaagatat acattcttta gcttttgcct
    14341 tactattcat tgtgtaattc caatgtccta agccataaac taactttgtc atttcttcat
    14401 cactcttttt aaacatctgc ttttcattat aaaaaagcat ataggctggg gtattaatac
    14461 cttttggaca tactttacaa taaccacaac ctgtacatat ttcgttcata ttcttattta
    14521 atctagtctc aatatcatgt atatcctcat ctgaataaat tctattttcg tctgcaattt
    14581 tacatgcatc atctatatct tgttttttat taaaccctat caaagatata cttatttgtg
    14641 aaattcctat gttgaatctt aaagctgatt ctatagtact ttcgccatct tttgatagaa
    14701 aagaaagttc tttattatac tttggaatag ttcccaaacg acttttagta tttaaaacca
    14761 ttgatttcac agcattttca ttttttactt taatcttgtt gtcagttaaa tgtcagcaaa
    14821 acattttatc cacaattttt atataacttt tccacagttt atccccagct ttatccacaa
    14881 aaaggtagct actattgagt gctacctttt ggcatattct tattcttcat cttcttgcaa
    14941 acttcttaac cattcttcaa gcgcatctat tttatcaata cttacctctt ctttatcatg
    15001 taattttgat attaactgtg ataaaggatt attatgtaaa cctccaaata gttttttagt
    15061 ttctgatttt aagtattctt tttcttttat taatattgta taatgtgtgt gtttacctat
    15121 tttttgagaa gctaaaaaac ctcttttagt taaccttaat aaaagtgtta gtgttgtagt
    15181 ttgtttccat ttatatattt tttccatttc atctgccaca tcttttgata ttacagtttt
    15241 ataaccagta ttccatatgt atttcataat cttgaactct gaatctggta attttttatt
    15301 tagcataata tccccttctt tctattaaca tataaaaata taaaattatt tttttcctag
    15361 tttctgcata ctttctataa atacttttat ttgtttctct gtcttttctt cttttttatg
    15421 tatagttgta aatacttgtg taaaaaaatt atcatattta cttacatctt tttcttcttt
    15481 tattacatct aaatattctt ttttagttac taatacttca tagtttaatt gaaaaccagc
    15541 tctcttcttt tttaatattc tcatttttac taatcttcca agtaaaatct ctgtagtgct
    15601 ttttctccac ttatattttt ctttcattgc tacttctatt tcttttttag atagagtaga
    15661 tttttccctt cacatgtact ccatcaacat taattcgccc ctgcgtaatt tactttctac
    15721 ctcttgtcgc tccatttatg tcacttcctt aattcatcta acttaaatta ataataggag
    15781 ctttttaaca caaaaactat aggtaagttt taccatatat tctactgttt tagtttgttt
    15841 ttatatataa aaaatattat tcacatctac gaatttattt aattttcaaa caatcttgtt
    15901 gaacaataac taattctatt gtattatttt agttgtagaa taaaactaaa tcggcaaaac
    15961 tagagaaatt tagtgacgca aagctatagg ggctaagact tatataaata tcttatgagt
    16021 tatgtcagcc agttgccaaa aagatagtgt tctttttgtt tttatgaaag ttttttaggg
    16081 ggataataat ttatatgttt aaaaataaaa tggataaatg tacacacatg ttgactgctt
    16141 atattagcag ttcgtatgat tattgtaatt ttttagatac acagttagat gattttatat
    16201 tagagtacgg agaaaatgta gtagagtctt gtttacacca agtgatggta ttggtaagta
    16261 agtataatta gatagacatt gatttctaga gaaccctttt tagttttatc tacttttaaa
    16321 taatgatatg ataaaaaatt aattaaaata gatttataat attcatagtt ttattttttc
    16381 ttaaaatgcg atataataaa gatgtaaatt cgtatcaaaa acaaaaaaag aaactacaat
    16441 ctattagcct agagtgaagt ttcattaact aaatattaat gtcgtttttt attaaacttg
    16501 atttttacat caagctcaaa gtcactcctg ccagagtggc tttttacttt tctggaaata
    16561 atatatgtaa taaagctagc tgttaaacta gctaacactc ctagtaaaaa ttcgctcatt
    16621 tctccacctc cttcctttta agggatggaa tgtagaaaat gaagctccac tcttagattg
    16681 tagttccaca agattattct tgcattttaa ttataacata attttacaat tatcaaatat
    16741 ctattcttca tattttttat ttatacaata tattctatat tgcaataaaa aacattgttt
    16801 taaatatata tatatatata cataggtata tactaaatat gttaaactta attatagaaa
    16861 atataaaatg aaaggaataa aaatatgact aagactatat tatgtgatta ctgtaataaa
    16921 ggaataaata aagatgataa taagtatatt acttttcata agaaaagtca tatgaaaact
    16981 aacatttgta ttaattgtgc attaaatttg atagataaag ataaattaaa tgaaaatatt
    17041 ataaataatc aacatgatta ttcaaagaaa tgaaaaagca ctctccataa tgaagaatgc
    17101 tctatataat aatgtttgac ttagtaaaga tgtgttgggg ttacatattt acttttttat
    17161 tatatcatat aactttgtgt atgaaaaaga atttaaatca attttaaggt gtgttgagta
    17221 atgttcttga tagtttatat gttgatgaat ttcaaaaaaa taagcactct tataaaaaga
    17281 gtacttttgg tatatattca agcatttatc taatacaatt atagcatgtc ttatgtttta
    17341 gtatgataat tttcgttcgt tttattatta caggttatac tatagtttta tgtatcctgt
    17401 taatattttt tactattttt tcaatgtttt gtttaagtat aaaaagagat aactatttta
    17461 attctagtta cctctttttg tgttactttg gccatataga tatacctatt gtaatagcca
    17521 ttgtagcaat tcctattata gtagttatac atagtccaat aatccattta ttagttgaat
    17581 caattctatc ttctatacct ttcatactat tctttatttc acttacatcc ttttctgtga
    17641 cctttttatg ttcgtttata gcttctttta aatcactttt taactcagct ctttctctag
    17701 ctaaatcatt ttttagttct attctatctt tgtctaaatc attcttgtat tctttaaaaa
    17761 taccctttat gtcattaaat tgcttatcta tactttcttt tacttccttg ttatcttgat
    17821 gcactctttt ctcagtctct aatattcttt tttctgcttc tagtattctt ctttcttctt
    17881 cattcatatc atctgcctcc tttaaagtag cagctataaa ttcactttga ctatatttgt
    17941 atttatcttt cgaagaatca ttatgataat ttttacattt atgtttttct atcttttttt
    18001 cattaaaact caataaatta aactcttttt ctttttttgc tcctaacata ctattcttcc
    18061 tttaataaat cagataaata gtttattaat gactctactt ctctctttga catctctaat
    18121 tcaagaaaat tattatcata tctaaatatt tttattataa aatcatcatc tctagaagtt
    18181 gaataaatag atgaaagatt aaaaggatga ctttttgaaa aataaacatt atctagtata
    18241 tcatatgtac tcttagaaaa attatacaaa tcatttttaa attccttact tacttttgca
    18301 ttttcatctt catcataaaa ttttcttaat tgtataccat actttcctat aaactctggg
    18361 tcttcaccta aaattttttc cgcctggctc ttgtactcat taacacaatg tggccatgtt
    18421 ggtttttgca tatacagatt gagtaataat attgccttct caaagatttt attatcttcc
    18481 ttatattttt ctatacaatc aggaaaataa tctataaaat cagttccatt ttcataagcc
    18541 attttaaatc cccctaaaat aatattattt aatattattc tacattagaa ttattgttat
    18601 tgcactacta ttttttattt cacctccatt attttatcaa tatatatatt tgttaactgt
    18661 ttatacacct agttttttgg attttaaaat ggcttatgat tatctttaat atatcatttt
    18721 ttattttata aattgtcgta atattggcat aaagtataag caaaatgtaa aatacattaa
    18781 ctatactcct tttatctatc aataaaatct aatgctttgt aaagtgtgtc aaatctatca
    18841 ttacccttta tcatagtata gtgttcttta gttatagaac ttatcttatt acatgctcct
    18901 ccaccgacaa catataaatt ttctgtctga cctggtatat aatcttttat atcacatatc
    18961 agtattttcc catcattata accccaacca actacagttg cagggatttt gtcaacttct
    19021 ccatcataaa cgattgtatg tttgtacatc tgtttaactc cctcattatt tatattttta
    19081 tttaatatac cttctgctat taatttagca actatgtctt tatgtctgat ataatagtct
    19141 gtatctgctt tactatctac aaaacatact tctattaata ttgctggtgc ttttgtatgt
    19201 ctaagccagt aaagccctct tacgtccgat tttgcacctc tattttcgaa tactgttgct
    19261 agtttctcgt tgaccctttc agcatatact ttaccattat tagttttata aattgtctct
    19321 gtacccatgg gatttagagt tatcttattt gcgttgaaat ggatttgtac agctaggtct
    19381 acatcttgtt tatttgctat ttgacattgc tctgctaagt aattactaga tttatctact
    19441 tttccagtat atacagtagc tccaccttgt ttcaaccatt taacaattaa atcagttagt
    19501 attctatttt cttttccttc gtctatatac ccagttgctc ctgttccttt tccacctaaa
    19561 gtgtgtcctg gtactattgc tattttcatt atttgttttc ctcctttaac tgtttgtaag
    19621 tttgatttat acctattgat ataccccaac aaattacacc ttgtaagact gcaacaggat
    19681 ttaatcctag catccacact gaaaaagcta ttccaagtat caataacact actggaatgt
    19741 atttattatc taattgttta tattttttac aacctttacc tataatagag agagcagcta
    19801 ctaaaattag caactgctct ggtataaaac ttattaaatt atccatctct tatcctccta
    19861 attaattaaa atattcctct ttgaactgca aatataaaga accctacaag tgttgtaatc
    19921 attgtaccaa ttagccattt gagcatactt gtaagtgagt ttagattctc acacaatgct
    19981 tttaactctg ctttagactc tatatttgct atttttaatt cgtctatttc ttctccatgt
    20041 ttatttattc ttgtttcatg tctttttaaa tctgcttcga aaagttcttc attcatgaaa
    20101 acctcctaat ttttgaatta aaaaagacta tgctatatag tcctctccta caatttcttt
    20161 atattcactt gctgttatct tattcttttc tactgccgtt ttaacttgct ctttagtcca
    20221 attaccatta ttatagaaat ctgttattat cttgtaccaa ttcatttata tcaccccatt
    20281 tgacattaat tgaaatgtta aatctgctat tgtttgttct gtagaattta ctttatcttc
    20341 tatgctactt ttaatatctg tatatctata gaaaacctct ttagtatcta tatttataaa
    20401 tagctttgct tctttattct ctgcatactt ttgagttggt aaactctcta tcaaaatacc
    20461 ttgttttaag ttttcctccg ataacaaatt tggttcatag tgtatcatac caacatattt
    20521 tatattttgt tcttctgtat ccattaaatt tcctaaataa atcataatga ttctcctttc
    20581 tcatccgaat aaaccttttt tgacaatata tttctaaaaa ttccactact aataaagaaa
    20641 actatatcat ttattacaaa cataccagca tttgggtatg tttcagtaag ttttcggtag
    20701 ctgtcgatat gtttaagagt atttaaactt atttttacaa aaggactact agaaagcgaa
    20761 tatatagcat atatatatcc attatatata tcaaaattgt catatttgct atcgtctgaa
    20821 tacacaatta aatttaaatt tgcgtcatat tttactaaag cacttttttt gttgctatca
    20881 atttctatac ctctttctgc atcagataca ataacaaaat catttaaaaa cttaatgttt
    20941 ttcatatgca aatatcctcc aattctaaaa tctttagcaa tggcaaaatc aaaatttatt
    21001 ttagataaat gacatgttgt aatactactt gaatttgagt gttctgttgt agcataaata
    21061 gcattcttat tgcaaacaaa cttacctcct ttaaaattgt aaataccatt agaaactaaa
    21121 tttttagtta acattatata catatcagat attctaattt tgtgaagtgt agaagaagtc
    21181 tcatctccat atactccata aataaactcc ccataagtac ataacttata ataagcacct
    21241 tctattgact gtacttcact tcctgttgac ttatttattt tatataattt agtattatca
    21301 gatataaata aatattcttg agtaatacat atgcatgaga agttagcatt agctaaagta
    21361 atgtcaaaaa ctactgtttc atcaatagca ttaattttaa ttagatgagt ttctttaatt
    21421 acataaaaat atggttcttc atattcaaaa gttttgaagt tcccactaca tctttcaata
    21481 tattttatag ctccatttgt aactaaatac gaatagttat ttgaaagtgt tgtttctctt
    21541 aaatctaatc tcccctcttt tatatcaatt ttattcttta tttcttccca tgtatccctt
    21601 gtagtaacct ctgcaccttt ggagtttaat gctgttacta cattattttt agcatcaact
    21661 ccagactgaa aaacctcttt taaagcatct tctacattat cacttgtaaa attattctct
    21721 gtatcctcta tagttacatt ctttgcttct aatacaagat ttctaacttt attaactaac
    21781 tctttaaaag tcatttagtc acctctttca ataaaaaaaa gaacctacta cgctgttggt
    21841 tctattcctt ctactactcc actctgttta attatataat cctctactgc ttttctatac
    21901 tctgtgttag ttacgtcatc taattcaaat tctcgatttt ttaaaggatt taagcctcta
    21961 cttaaaatcc tctctgctaa tattcttact acaacattat ttatattcat tataataatc
    22021 ctcccacttt ttcattttct gcaattagta attgattttc taactcttgt attctctttt
    22081 cttcttcgct taaatagatt ggaatatctt ttaaaatagg ttcttttgtt attggattta
    22141 tagattctat atactgttta ctatagtcta tatttccata ttcaacatca atataatgta
    22201 attctgttat tgtatcatgc tctaatatat ctcctgttgc ttctccagtt tgtaaaagta
    22261 ttttaccagt ttggtcgtaa attattctat ttgctctatt cattttatca cctcatttat
    22321 ttaaatttta tagcatacca agaaaatcca taaataggaa gagaagtcct acttgcaggt
    22381 acattaattc cattactatt aatataagca tcttgttcat tgtttatata aaatcctgca
    22441 tgacaattaa aatcttggtc atccatttta cctctagtaa gttgaagatt agctataaaa
    22501 tctcttttat ttgtagacca agacatgtta caagtagcaa taataatatt tttctcaatt
    22561 acactaccag tgctaacagt ttcattttca gcaataaaaa tattaggaac aaatcctaaa
    22621 ttattaatat taatccaatg atatggttgt ctaatatatg ttgctccata taatccgcca
    22681 tataaattaa catgttgaga actttgtata acagaagtag tcccacttgc aactttatat
    22741 tttgtattta actgtgttat ggtattgtta gcttgtgtta actggttcat caaatcctgc
    22801 aaactagcgt ctccactatc aaatgatgcc tttattttct ctgacaactc aactagtgtg
    22861 ttatttagat ttgcttctat attctttaat gctaaagtat ttataatact tgttttacca
    22921 tttttaaacc cttctccaat ctctaccaat ttagttgata tatcttgtaa agttgcatca
    22981 gattgaagtg gcataatctc tttgcttata cttaaaacct tctcaactgt tgcattttct
    23041 gtgtctgtag caactatttt taatgtgtgt attgcattgt ttgtaagttc atagtttatt
    23101 gttttctctg ctgttaaatc tgttgttatt gtttctttta atacatcatc taaataccac
    23161 tcaattttac ttaaattatt atctaaatca gatgctgtaa attttgcaga attagaatta
    23221 tatgaattaa ctaaaatagt tggtttatta tttattcttg taaatgttgc ttgcttactg
    23281 gttactactc caccttctgt gctaatttct attaagaaag tatttttaga accaatttct
    23341 aattttaata tctgctcatc tgttaaaatg gtagtgtact ttccatcttt tgaactaacc
    23401 ttagttgaaa caacctcatt attaattttt tcagttactg taaatggata acttgatgca
    23461 aagtcatcaa atattctata tgatatttca caacctttac ttatatttgc atccatatat
    23521 atttcaattt taggtgtatc tataaaaaga acttctaatt tttgctccat tgtcaagttt
    23581 tctaaaactc cattttcacc attaataaca cttgataatt tagataaatt caaatttctt
    23641 gtaacagata tattttttac actacttaat tcaaatactg cttctgccct acctcttcct
    23701 gctggataaa atacatgtga tgattcaaat atatcttgct ctgaataaat tccttttctt
    23761 ttccttttac ttgtttcata agaaaaatct atcactttat cacctcactt tctacattgg
    23821 tattaaatta ttatttatac tagatataat attactatta attcctgtta gctgttctgc
    23881 taccttacta atttcttcat gtatcttttt agacgaataa gtagtcattt cagacactct
    23941 gttatcatct acagttgcat taataaaatg agtttctgca tttccattta tcacataaac
    24001 gtttaattct gaccttgttt cacttctaat ttctatagta ttatcatcta taattttaaa
    24061 gtttgtaact acattttctt ttgtagtagc atctataata tttacaacta ttctctgtgt
    24121 taataaacta tgtgttacag ttgctttgaa tccattttct gcatcctcaa cccaatcgtc
    24181 aattgttatt gtttgagtag atgccacatt tgaaccacct gcgattaatt ggtcaatttt
    24241 aatattttgt ttctcatttt ctgtgtcaat tctagtgttt agctctgttt tagtagtttc
    24301 tatgttgctt gttaattctg ccttagttgt atctatttta gtattaacag tacctatttt
    24361 agtgtctagg tcctgtaaat cttttaatgt ggcaaaaatt atagttgggt caattttaag
    24421 ttctatgtta tttacattag atacaacaaa gatagttttt accttcatgt caattactgc
    24481 tccatcttcc actttaggtt tatatgctga cctatacttt gatatagcta gtaaattacc
    24541 ttcggtatct aaataaccta tctctctaac tacaaaccca ccagcactag caggtattaa
    24601 actttctaaa attaagcaat ttgtcatgct ttcatcagtc ttgacattcc ctactttacc
    24661 ttcccaaact acatttttta atgctgtttg ttcctctgta ggattatact catttcctcc
    24721 accatcacct aattgtatcc tagcaaagtc aactttttcc cctactatac ttgcatttgc
    24781 tatcttagcc ttacctatat ctgttaatat cgtgtagtaa cttttatctg tagccaaact
    24841 accacctcct atctaataaa ttgtaacctc ttgatatcca aacccattgc cactaagtat
    24901 attaatctct ccttgtgttt caatatcact aggtgaccat gggtaaattg taatctcatg
    24961 ccctgtaata gtggttgctc caaagtacac actattatct ttgctaacta aaactctagt
    25021 gtaatctagt gtcatgttgc atggcttaat atcacttaca aaagaatgaa cttcctcaaa
    25081 ccaatcttga tttctagcat cactttctaa atgtatatta taagtagcat tatttatagt
    25141 taactcataa ttaccttctc caactatact atctagcca
//
LOCUS       DAKBNN010000037        24942 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000037 DAKBNN010000000
VERSION     DAKBNN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.037"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            171..1061
                     /locus_tag="OUB02_001323"
     CDS             171..1061
                     /locus_tag="OUB02_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCT8885092.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1279..2565
                     /locus_tag="OUB02_001324"
     CDS             1279..2565
                     /locus_tag="OUB02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT8885093.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTKKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2654..3097)
                     /locus_tag="OUB02_001325"
     CDS             complement(2654..3097)
                     /locus_tag="OUB02_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HCT8885094.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3121..3915)
                     /locus_tag="OUB02_001326"
     CDS             complement(3121..3915)
                     /locus_tag="OUB02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HCT8885095.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(4481..4957)
                     /locus_tag="OUB02_001327"
     CDS             complement(4481..4957)
                     /locus_tag="OUB02_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCT8885096.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(5058..6227)
                     /locus_tag="OUB02_001328"
     CDS             complement(5058..6227)
                     /locus_tag="OUB02_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8885097.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            6390..7253
                     /locus_tag="OUB02_001329"
     CDS             6390..7253
                     /locus_tag="OUB02_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCT8885098.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            7324..7917
                     /locus_tag="OUB02_001330"
     CDS             7324..7917
                     /locus_tag="OUB02_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HCT8885099.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(8168..8803)
                     /locus_tag="OUB02_001331"
     CDS             complement(8168..8803)
                     /locus_tag="OUB02_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT8885100.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(8883..9614)
                     /locus_tag="OUB02_001332"
     CDS             complement(8883..9614)
                     /locus_tag="OUB02_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCT8885101.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            9945..12134
                     /locus_tag="OUB02_001333"
     CDS             9945..12134
                     /locus_tag="OUB02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCT8885102.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            12545..14473
                     /locus_tag="OUB02_001334"
     CDS             12545..14473
                     /locus_tag="OUB02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HCT8885103.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            14460..14807
                     /locus_tag="OUB02_001335"
     CDS             14460..14807
                     /locus_tag="OUB02_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HCT8885104.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(15049..16968)
                     /locus_tag="OUB02_001336"
     CDS             complement(15049..16968)
                     /locus_tag="OUB02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT8885105.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(16994..17800)
                     /locus_tag="OUB02_001337"
     CDS             complement(16994..17800)
                     /locus_tag="OUB02_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCT8885106.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(17859..19307)
                     /gene="ddlR"
                     /locus_tag="OUB02_001338"
     CDS             complement(17859..19307)
                     /gene="ddlR"
                     /locus_tag="OUB02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HCT8885107.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIATIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(19331..20230)
                     /locus_tag="OUB02_001339"
     CDS             complement(19331..20230)
                     /locus_tag="OUB02_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCT8885108.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            20488..21744
                     /locus_tag="OUB02_001340"
     CDS             20488..21744
                     /locus_tag="OUB02_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCT8885109.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            21802..22581
                     /locus_tag="OUB02_001341"
     CDS             21802..22581
                     /locus_tag="OUB02_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT8885110.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(22685..23269)
                     /locus_tag="OUB02_001342"
     CDS             complement(22685..23269)
                     /locus_tag="OUB02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HCT8885111.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(23469..24899)
                     /locus_tag="OUB02_001343"
     CDS             complement(23469..24899)
                     /locus_tag="OUB02_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885112.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         8998 a         3134 c         3748 g         9062 t
ORIGIN      
        1 tttagctata attattattg ttattaattg aaaagattta ttagttactt gacatagttt
       61 acacttttaa ttatagtata aattggaata tttagataat tattatttaa tgtataagct
      121 aagtttttaa ttaatatggc tttatacatt tatatagaaa agaggtacgt atgagtagca
      181 caatcaaatc aaatcgaata aaaggtataa tgtttataat agcatcagcg cttggctttg
      241 caatgatgtc tgcattcgta aagctagctg gagatttacc aagttttcaa aaagtgttct
      301 ttagaaatct ggtatcagct ataatagctt tttggcttat cataaaacat aaaggaagtc
      361 taactggtaa aaaagaaaat ttaaaagtac ttttatatag gtcaatattt ggaactctag
      421 gagttatatt taactactat gcaattgaca agttgatgct ttcagatgct aatatgctaa
      481 ataaaatgag tcccttcctt gtagtaatat tttgtgcaat tgctctaaaa gaaaaaataa
      541 atgtaaagca gataggagct attataattg catttatagg agctttattc ataataaaac
      601 caacatttag tgtagaagta attccatatc ttgggggaat tgcaggagct gtatttgcag
      661 caatggcata tacttgttta agagttcttg gagatagaga agattactat acaattgtat
      721 ttttcttttc agttttttca ttagttactg tagggccaat tgcttttgca gtatatgaac
      781 caatgaccct tatgcaactt atatatctat tactaggtgg agtatttgca agtctaggtc
      841 aatttggaat aactctagca tataaatatg cacctgctaa ggaaatctct atatttgact
      901 atagtaatat aatcttttca gcaattttaa gtatattctt attcaatgtc tatccagata
      961 tgttaagtgt tgttggatat ttaatagtat tttcggcagc attttatatg tttttataca
     1021 ataagaagtt agataaatta gacaaagaaa agaagaagta aactagataa aagtgttttt
     1081 ataatttgta ttgaatattt aagtctttgt attaaaatta aagaaaattt aattaaaaat
     1141 gtttggtata ttaacttata acagcagtta aaattgaaaa attataactg ctgtttcttt
     1201 tattaaaaat atccaaatgt ctgtaaaaat agactataat agagtagtat aaataatata
     1261 aaggggataa attaaagtat ggaaaaagtg aaagttgctg tgattggagc aggtaataga
     1321 ggaacgtatg catatgctcc atatatttat gaaaattctg atgtatgtga aatagttgct
     1381 gtagcagaac ctaaaaaagg aagacgagaa ctatttacaa aaaaatataa cctagatagt
     1441 aaaaatgtat ttgaaacttt agaagacttt tttaaacatg acaagatggc agatgcagta
     1501 ataatagcta caaatgatga tagacattat gatgtagcaa aactagctct tgaaaaaggt
     1561 tatcatgtac tattagaaaa gccaatgtct aatagtttag atggtttggt acatatagat
     1621 gacttgtgtg ataagtataa agataagatt tttatgattt gccatgtact gagatattct
     1681 ccattttata ataaattaaa agaaattgta gaaagcaaaa aattaggaga gctagttagt
     1741 atacaatata atgaaaatat aggatattgg cattttgcac atagctttac tagagggaat
     1801 tggagaaata gcaatgaaac cagtccttta attttagcaa agagttgtca tgacatggat
     1861 attttgttat atttagtagg aagtagatgc aaaaaaatat ctgcatttgg aagcttaaag
     1921 catcttaata accaaaatgc tagtggagag atggcacaaa attgtcttca gtgtttagtt
     1981 gaaaaaaaat gtccatattc agcaaagaga atttatttag aaaaagatag aagtataaat
     2041 agagctgttc acataaatcc aactgaagag aatctattaa atatacttaa gacaagtcca
     2101 tatggaagat gtgtttacag atgtgataat aatgttgtag acaatatggt aaatatacta
     2161 cagtttgaaa atggagtgac ggctactttt aatttatgtg catttactaa agaaaatgga
     2221 agaactataa agttaatgtt tagccatggt gaagttggtg gggatttaaa taaaaatgag
     2281 ataagaatta aagaatttgg aaaaaacgaa gaaatagtta tgaatccatc aaatcaacag
     2341 aatatggtag aggaatatga tagaaatctt atagctgaat ttattaaatt agtttcaaat
     2401 aaagaactag aaaagggtag agtagcagca aaagaagcta tacaaagtca tgtaatggca
     2461 tttgcagcag aatattctag ggtcagtgat gaagtagtgt atatagaaga attttttgat
     2521 agtgctaagc aaatgactaa ggagatagaa gaaactatat tttaattaat aaaaatttat
     2581 taaataaaat aaagatagat taaataatag aaatacgaaa atatgtgttc tattaaggaa
     2641 tctatctttt ttattaaggt tttttcttat ccctatcaaa aaaatccata cattctgata
     2701 aaagataaga aagtaaagcg aataagattt ctgtacttaa aggaatcgtt actgaatcca
     2761 taaaggtatc aattatgttc atagctagaa aagttagtat aaaatcaatt ataaattcgc
     2821 aaagtttata tattgagtat ggtaatttgt ttacatatct gaatatatct aaaatagtag
     2881 tgcatagaaa atcaattgga gtggttatac aaaaatatat taaaaagaaa attaatacag
     2941 ctccaagaga tttatattca aatcctaaga atgataagaa gccaattcct ataaccgtga
     3001 aaatacaaaa agttatacca aaaaaaacag caaaaaataa aaatacagaa ataaattcgc
     3061 ctaaaggaat ccttctttta ttacttatct taatcaactc cttaatttat gatatgaaat
     3121 ttatataaat aataacattc ttaaaaattc ataatagtcc tctgtttcaa atagtactgt
     3181 ttgagaatct atttttttca tatttggata aaaagaggcc ttaaaagctt tataatgttc
     3241 tctaaatttt atttctatct taaatttatc tggcaatttt attaagtgcc tagataaatc
     3301 tccagataaa gcttttttga cacctttttc aatttttttt agagcaaggt ttggatggat
     3361 tgaaattgaa ccatttccaa ttccttttga cacaggaaca gtgactatat ttggatttag
     3421 tttttttgca ctctcacaaa gcatttcatc tccacttaaa aatgctacag gaacattata
     3481 atatgcagca atataagcat ttatagtaaa ttcagatgct atttcatcat ttattttaaa
     3541 atagtcataa tcaatatcca tagtatgaga aagtggatta ccattacaag tagctccaga
     3601 gtgatagccg gtgaatatac atgcatcaaa gctttcatca attcctgcca tcataactag
     3661 tgggtctttg gtccatcctc taagtatttt gacgttttca ggtaaaatat ttggattaat
     3721 atttctacca ctgtcatgtg catctttaac aagtatttca tgtacaccag attgagtagc
     3781 tccatcacat gctgctgtta tctctttact catttgtgtt ctatagtgtt gcgaaaattc
     3841 acattcaagt tttgtttcat cccaatttac aataccacaa gtaccttcta tatcagcact
     3901 taaatacaat ttcataatat ataccccctt atgtttatta ttgatgataa taacagataa
     3961 cagaaaatac ataatcaata aagggcataa aaatattatg tcttactatt aactattatt
     4021 aaatctgtaa ctgaaaaata actaaattgg tataaaaaaa tataacacta tgtgtcattt
     4081 atttatatag ttttttatat ttgatgttta tataaaattg taacatataa gtggttacat
     4141 ttactagttt aacatagtta aaatagttag gtattatttt taagttaaat tgattattat
     4201 aaaatcgcag aataaaagaa aaatttaatt tattaaaatg catgaaatgt tttttcctca
     4261 acataatgct aaattaaact caaaaagaaa taatagaaaa atcttatgta gcttagaaag
     4321 aatgtgaatg atttaaggga aaaatagaaa tttattatat aatattaggt tgtattatat
     4381 aatacagcct aatattatat gtatatgtga cataaaatct tggtaagtgt aaataaaata
     4441 aatgtggaaa acaagttaat gaaatcaaga aaaaagtaat ttaaaattca atttcatctt
     4501 cccaattaaa ttctcctgat ttttctacac tcttaggcca gtgactacac catttaggaa
     4561 cggacggatt ttcaacccta tcaacgcttg gagtatatgg ctttaagtca ataacaggac
     4621 tattatcatt agcatcaata tatggtattt ttattatgcc attttcatgg tcgatattga
     4681 taatttgtac agcagtcaat gctattggat tgggtcttat aggtgacctt gttgcaaata
     4741 ttcctataac gtctggtcca cttttataag gttttggggt ttctaaaata cttcttagtt
     4801 caggattatc aaacccatca gcccaccaaa atacatttaa atgactgaaa tcgtcgagtt
     4861 catttaatgc aggtacatac tttttgtcta atttaataaa gacctcttcg ccttgaatac
     4921 atattttacc tataggattt attaatattt ctttcataat attacctcca actatttaat
     4981 ttatcatttg taattttagg gtataacatg acatcatgtg agggtcaata taaaataata
     5041 aataaaaata ttaatagtta gaatgataat acatttttta catggagcat gacaccagta
     5101 ataaggcaat cttcgtcaat atcaaaagta gaggtatgca aaggggaagt aatgtttttt
     5161 tcacgatttg cacaaccaag gtggtaaaaa gctccttcac agtgtcttag aaaatacgaa
     5221 aagtcttcag ccccaagagt gggcgagtct ttaaggataa tattttcttc accaaacaat
     5281 tctacagcat tttgtttgac gtaatctact agatggtttg aattgataag agctggataa
     5341 ccttcctcaa tttcaacaga aatacttcct ccaaatgttt tacaagtaaa atcgcaaatt
     5401 tctctaattc gtttttttga aaactctcta gtttcaggag tcaaggttct caaagtaccc
     5461 cttataacca ctttgtcaca aactatattt tctttgattc caccttcaat ctttcctaga
     5521 gaaataacca cagaatcagt tggatttgtg tttcgactaa cgattgtctg tagagctgta
     5581 ataatatgag cagatataac aatagcatca ataccttgat gtggataagc tccatgacat
     5641 tttgaaccat gtacagttaa ttgaagtgta tcggtggaag catttagagt gttgtatttt
     5701 aattcaatta gatttttgtt gatattggga tttacatgaa gtccaaatat atattttaca
     5761 tttgggtttt caagacatcc atcttctatc ataagttgag ccccaccaat agtttcctcg
     5821 gcaggttgaa aaaagaattt tacatttaca tttaagtaat cctttaattt aaatagtata
     5881 ttacaagtac ccagtaaaat agcagtgtgt gcatcatgac cacatgcatg cattttgcca
     5941 atattaatag atttataaga ataattaagt tcttctgtta taggtaaggc atctatatca
     6001 gctcttatag caactgtttc aaagtttgga ctcacgttaa tgtatgcagt tatccctgta
     6061 tgattcttat attcaatata atttatacct atttcattta agtatttaat aatttttttc
     6121 ttagtttgaa attcttctaa tccaagttct ggagtcttat gtaaatctct tcttatattt
     6181 ataacccaat catttatctc atgacaaaga gtttttatat taagcataaa acacatccta
     6241 actatattaa aaatttattt ttcttcatta attatattat tttatgtaaa tcattattat
     6301 gtatcaagta caaataaaat atcttataat tatattagag atatttagta tggagaagaa
     6361 tatatcattt tataagaaga ggtgtttaaa tgttaggaat aattgcgcca tctgggccat
     6421 taagaaatac aagtttggag gaaataaagt ttaatttaga aagttatggt tacgaagtta
     6481 agttttctga aagttgtagt ttaaattata aaggttattt agctggtaat gatgatatta
     6541 gagcaaggga tatagaagat atgtttttag ataaagatgt agatataatt atgtgcttaa
     6601 gaggaggtta tggaacaaca agaatattgg ataaaataaa ttatgatata ataaagcaaa
     6661 atccaaaacc atttataggt ttttctgata taacaggttt aaatttagct ttctataaaa
     6721 attgtggtct tttaccatat catggaataa tggcagcaaa tgtaggtaaa tgggataaat
     6781 ttacctataa atcattggta aatgctttag aatttaaaga tgaattatat ttagaaaatc
     6841 caaaagaaga aaagatttat actgtatgtg aaggtaaagc agagggaata ataatgggag
     6901 gaaatttatc cttaataatt gccacaatgg gaacaaaata tgaaatagat gcaaaagata
     6961 aaattttgtt tattgaggaa attggagaac ctcaatataa attggataga atgttaacac
     7021 aactatattc ttcaggaaaa cttgaggaat gtaatggtat aatttttgga gattttaagg
     7081 attgtattga agaaaatgat ttgatggaat tactaataga atttgcaaat aaggtcaata
     7141 aaccttctat atataatctt cagtcaggac attgtatccc aatgataact attcctttgg
     7201 gcagaatgtg cgaattagat gctacagaaa aaatcgttaa attaaaaaaa tagtgtgtat
     7261 ataaatataa tagaaatatg gtataatctt atacaagtaa aaatttagct aggaggatga
     7321 aaaatgtctg atgatagaat aatagacttt aacgaactaa agaacaaagt taaagattct
     7381 gatgtagata aatttgagca atacatatat aacttatatt tttctgttat ggatggaaag
     7441 atgtctatgg ctgagttttc aagaaaaata ttcgattata tgagagataa caacatatct
     7501 caagaaaaat ttatgaaaat acaaaagcaa tttatggaga gatatggtat ggacactgaa
     7561 gaagtagaaa aacaacttag aaactttgga atcgaccctt ctacagcagg ttttatgagt
     7621 aataatactt ccagcaaagt atctacagaa gatttagagt catttaagaa gagtgcagga
     7681 ttttatgaga aatatggaga aaaaatccaa ccaaagagtt gtataacaac atttataaaa
     7741 aatgacttaa atgatattaa tgtaataatc gaccaagaaa agataatgct ttgtagtgat
     7801 agaaaaataa atcttatgga ttcagaacta aatgagtttt tattagaata taagaatatg
     7861 tttaataaaa aaataaaggt agtaatgtgc gaaactacga ataagtatga ttattaaaaa
     7921 atgaatttaa atactgaagt agtatcaatt aattgtacta cttaaaaata aaaatcgcaa
     7981 tttattattc cttataaatt tgtataagga atatctaaac tgcgattttt tatattgtga
     8041 agattagtaa taatagatag tgttttaaat tgtaatcttc tcactaaaac tgtgtttttc
     8101 tattagaaat tttattaaaa ataaatatct gataattgta ttatatggga tgtaaagtat
     8161 tagaagctta acgaatctct tttaatacat taactccatt ttgaatcata ctgttgaaaa
     8221 atactaagtt taaaatctca gcagggtgaa cgttaggaat atcataagta tctactaaat
     8281 tcataggtac aactatattt ttttcaatat tatgttgatt aaaatatgat tttagagtta
     8341 ttgcaaattg atatatacat atatctgtgc aatcgccaac tataattata ttatctatat
     8401 tatcaaaatc caaattttct aatgcaaaaa aaccattagt agaattttta ggaagaattt
     8461 gtaggttttc tatcgactta agttcatcaa ccaattcact ttctacatca ttttccaaac
     8521 agtgaacagg ataactcaaa agttctattg atttaggagt atgagaatca gtaaatgcta
     8581 ttactctatt taatttgttt gatatttttt ttgtaaacat ttcaattggc tttattaaag
     8641 actctactct aggagagtat aatgcacctt gtcttgcaaa gccattatta acatcaatta
     8701 taaataaagc agttttactc aaatcataat tttctatttt ttctattggt agattatcta
     8761 aattactctt taaagcttca agttcattta ataagttatc catagttata cctccaagat
     8821 aaatttaata aaattgtaca tattaattat ataaaagtca aggagtacaa actattgtat
     8881 aattagctgt attgttttga gtgtaaaaga tttgaagcaa tatctggata gttagtaaaa
     8941 ataccatcta cattgagttt tagacagtgt agtatatcat cctcttcatt tacagtatat
     9001 acattgacac caagtagatt tttgtgacaa agttcaatta gagattcatt tactaaaact
     9061 agaggtggat gaaggtaact attacccaga aatttagcaa aattgactac attgtttata
     9121 ggtaaataac acaataatcc tgtttttata ctagggtcta atttatgaaa gttttttaag
     9181 ctattgctgt tgaaactaga tataagaact ctatttttca tattatactt taaaataagc
     9241 tctaaaacgt ctttttctat attgggatag tttattttgt cagtttttag ttctatattt
     9301 aaaataaggt ctgtaggtga aattagaatt aatacttctt ctaaagttgg aattttacat
     9361 tctttattgc tttgatatcc ttcaaaagat gagtttagag tttttaattt tcttaaagta
     9421 aagtctttta caaaaccatg tccattaaaa gttctatcaa ccttttcatc atgaataact
     9481 acaatttttc catccttagt tctgtggaca tcaagttcta ttccatatat atccatatcc
     9541 aaacattttt taaaagctag aatagtattt tctggatact ttccactaaa ccccctatga
     9601 gcataaatat tcatttatat ggttccctcc tataagtata acttatctta taaaaattta
     9661 attaatataa tatatcatat tccaaaaggt attgttatat attagttaaa taactatttg
     9721 taatttatat attatataaa tgtagaaaaa taaaatcata tatgtgttat aatatatttt
     9781 aaacattgta aatattacga atttagtagt cataataagt gtaattttat gcaagctcat
     9841 gtttttagag cttgcatatg tattattaat atatttatat attgtgaaaa taaaattcca
     9901 aaagaatttt ttctatagac ttagtagtaa gggggaattt tgatatgtat gaagttaatt
     9961 tagaacgagc tatgaattat tggatggatt tgttagatga ttatacgaat atagcaaaaa
    10021 ttccaaatga ttttttttat aaagattgtt ctgatgaagt agtagatgag gtttttaaga
    10081 tgaatattga gaaacatgaa aggttaattc aatattctaa agtgaacaat attagcatag
    10141 actctatcat agaaactata tatggcctta tacttcaaaa gtatatatat gaagatgatg
    10201 tagtttttgg taaaacaatg aatataattc caataagagt aagagttaga caaagtgaaa
    10261 aatttataga tgttgtaagg aagctaaaaa ggcagtggag caggtcttta gagtatggat
    10321 actatttgtt atcagatata gagaataaaa atcatctaaa tactaagctt ataaacacaa
    10381 tatttatagc taaaagtttt gatttatcat atgattatga catagaaaaa aaaggatatg
    10441 atttatctgt aatagtatat actaagaggg cacttaaaat aaaaataaag tacaaaccat
    10501 ctatatataa aagagaaact attagaagaa ttttaaatca atttgattat ataatagagc
    10561 aaataataga agataatgat attttaatag aagatttaga atttgtatgt aaggaagaag
    10621 aaaaagtatt attggaagac tttaataata atcaatatga agtaccctat gataaaagtt
    10681 atgttgattt atttagggag caagttaagg taacacctag taatatagct gctagggatg
    10741 aaaatagaaa gttaacatat aaagaattgg atgaagtaac tgataaactt gcaggttatt
    10801 taaatcatat aggagtggaa agtgaagaca tagtagctgt aatgttacca agagatatta
    10861 acataattat tacagcaata gggattatga aatcaggggg agcatttttt ccaatagaca
    10921 caagtaatcc agaagaaaga ttgaattacc ttcttgaaga tagtaatgca aaagtagtga
    10981 taactacaga tgaactcaaa agtaaggttg taaatgagaa taccatagta ctcgatataa
    11041 atgatgaaga aatgtttaag cttggatatg aattaactga aaaaataaca tcatcaaatt
    11101 gtgcatatac gatatctact tcagggtcaa ctggtaggcc aaagaccata gctatagaac
    11161 ataagagttt agtaaatatg tgttattata gcgtaaaatc tataagcgct acagaaaatg
    11221 atatatgtgg tatatactta agtttcagtt ttgatgctgt aatgaaacaa ctattccctt
    11281 atttgcttgt aggtgcatca atagatatta tgcctgaaga agctaaattt gatgaatata
    11341 cagtaaatga atactgtgag gaaaatgaca taactatact agcattgcca acagcatttg
    11401 caaggttatt tatacaaaat tgcaataata attcacttag agttgtacag actggaggag
    11461 aaagattaaa aggatataga aagcgtaatt atgaactata taatgagtat ggaccaaccg
    11521 aatttactgt tgtatcgact tcttttcatg tggacagaga atatggaaaa ataccaatag
    11581 gaaaacctat atttaatact tatgcatatg tattagataa aaagaacaaa ctatgtccaa
    11641 ttggagcacc aggagagcta tgtctatcag gcattcaaat atcaagagga tatttaaata
    11701 aaaaagggct aacagagcaa gtatttgtag agaatcctta caaaacttgt gaacataata
    11761 agttaatgta tagaactggt gatttagtaa gatggttaga tgatggaaat ttagattata
    11821 ttggaaggat ggacaatcaa gtaaaaattg atgagtttag gatagagcta tatgagattg
    11881 aaaatataat aaataatatt acagaaatta aaagtgttgt ttgtatatcg agaacaaatg
    11941 atgatggaga tatgtatata tgtgcatatt atgtaattga tgaggaagat agtggtaaga
    12001 taaatgaaag aactattaga gaatatttaa atgagcatct acctccatat atgattccaa
    12061 caataataat gaggatagat aagataccag ttacaccaat cggaaaagta aataaaagag
    12121 cattaccaga ataatttttt attatgatag gtgtcataaa gtgatttcag acatttcttt
    12181 atgacactta tttttataaa agaatcaatt tgcataaaaa caagaatttt ctgtatatat
    12241 ttactatggt gaaaatttat ttataagaat gattattgta ttttttatac ttttccgtgt
    12301 tttaaagcat tttttttaca aaattaattt tacattaaaa aattttttta ttttatatct
    12361 taatagtttt tagtagaatt gaaaaataaa aagaaaatta gcataaaaat atctaaaagt
    12421 agaaattaat aatatagggt taaattttag tagttttgac tcgctatttg taaaataata
    12481 aaaagttaag ttttatatgt atatatgtta taatatatac aaattgaaaa ggggtgaaaa
    12541 ttttatgaac aaaaaattac ctaagggtgc ttatggtgag gttagtggaa aagattacgt
    12601 tccatatatc acagataagt ccagaaccgg tggaaatgta gctgtactga ttatcggtat
    12661 tattttagct gccatttttg ccgcatctac aacttactct ggaatgaaag ccggtcttac
    12721 agttgccgca ggtattccag gagctataat aggttctgct tttgttggag catttgctcg
    12781 ttcaaagggt attcttggta aaaacttaat ccaaggtatg tcaagtggtg gagaatctgt
    12841 tgcaagtgga tttattttcg tattaccagc agttatatta attggaagcc aaattacatt
    12901 cttagaaggc ttagctgttg gagttggtgg agttttattt ggtataggaa ttgctgctat
    12961 agttcacaac tatctaatag ttgaagaaca tggtaaactt atgtacccag aatcaatggc
    13021 tatatctgag acacttgttg cttcagaggc tggtggagat tcaattaagt acatgggaat
    13081 tggatttgta ataagtggat ttattactgt attaactggt tcattcttaa atgtagcaaa
    13141 caatgtaatg agtcttgtag gaagcaaatt ctacaaatgg aaatttgata ttgaagtaaa
    13201 tcctctactt ttaggaattg gatttatagt agggcttgaa gtttctttaa caatgtttgc
    13261 aggaagtatt ttatcaaact ttggtatagc acctttaata ggatacttta cagatatggc
    13321 taaagatgga gctatggtat ggaataatcc tgcaatgcct cttaaccaaa tggatgttgg
    13381 agcaatatct agtagttatg taaaatatat aggtgctggt atgatgcttt gtggaggtat
    13441 tattggtgca ataaaactta taccaactat aatcgcttct ataaaagaaa cacttaaagc
    13501 taaatcaagt gctggagaag gagaagaagg ttcatctata caaatgatac ttttattagg
    13561 tggagtagtt attggatttt tagcagcatt cttaatttct ggtaacattg ttatggcaat
    13621 tattggagct atcatatctc ttttattatc tttattattt gtcatagtag ctggtcgttt
    13681 aacaggaaca atcggaacat caaatcttcc agtttctggt atgactatag cttctctagt
    13741 tattgtaaca ttagtgtttg ttataatggg atggactgat ttagaagcta ataaatcatt
    13801 acttttattt ggttcattta tagttgttgc tattgctata gctggaggtt atactcaatc
    13861 tcaaaaagta acttatatta ttggtggtag caaaaacgaa atgcaacgtt actttacaat
    13921 tgcaagtata gttggtgtta tagttgttgt aggagtaatt ttattacttt ctgaccaact
    13981 tagagcaact ggtgataatg tacaatttgc attaccacaa gcaaacttaa tgtcaacatt
    14041 aacttctggt attatgtcag gtagtttacc ttgggttatg attattgttg gagtatttat
    14101 ggcaatcgtt ctatatgcat taaatttacc aataatgact atagctatag gattctattt
    14161 accaattgca acaacttcta taatattagt tggagcatta attcgtttat ttgttgaatt
    14221 agtatctaaa actgaaaaag aaaaagaagt taaagtttct aatggtataa gtttatcttc
    14281 tggtcttgtt gctggtggtt ctatcattgg tttaataggt ataatattac aagtaactgg
    14341 agttgtaact ccaaaggtac caagtggatt tgctgcaaca aattcaatgg caattgcttt
    14401 attagtagtt ttagttgtgc ttacagcatt acctattgtt ttatctaaag taaaaaataa
    14461 tgagcaagag taatgatgtt ttagatattg tatttaaaat tactgatgga cttgaatatg
    14521 aagtagctga aaatagtgtt gttactatac ttgaaaaaca ggaccataaa attcaaaagt
    14581 tttttagaaa gttaaaagct agaataccta aatacaaaaa aatagaacta gatgaatatt
    14641 caagttttgt ttttttacaa attgatggtc aaaggactgt tagagaaata ggcaagaacc
    14701 tagaagctaa atatggagaa gaatctcatc cactttatga aaggttgtta gtatttttaa
    14761 atcatattga agttaattgt cactatattg aaagactgga tatatagata taataagaat
    14821 tattatagat atattagagc atataaatta gaaggtaatt atgaaagagg ctgtctcaaa
    14881 atagagataa gcctcttttg tattgttgaa atgatgaaaa tatatttgat ttctcataaa
    14941 aaagagtgtc tcataaaact aaaaagttca ttttgagaca cttcctttaa aatatttata
    15001 aattacagat ttaaagcaaa acttattaaa cttaagatta ttttaatatc acttattatt
    15061 agctatttca tcagctagat tatttaaata ttcccatctt tcatatttgt attcaagttc
    15121 tttttctagt ttagatttct catctaaaat ttcttggagt ttagtaaaat cagttgaaaa
    15181 ttttgcagta ctttcatcaa gtgttgcaat tttagattcg agtttttcta tatcagagtc
    15241 aatattttca aactcccttt gttcattata tgagaactta agctttttat catttttagg
    15301 tttttctttt tttagggtag tagagtcatc ttttttaact gattcccttt tagattcttc
    15361 aaattcaatt ccttgaatct ctctataaat aagataatca ctgtaattac ctgtatagat
    15421 atgaattttt ccattacctt catatgcaaa tattttatta caaattctat ctaagaaata
    15481 tctatcatgt gatactgtaa ctactacacc tttaaattcg tctaggtatt cttctaatat
    15541 tttaagcgtt tcaatatcta aatcatttgt aggttcatct aaaagaagca cattaggagc
    15601 tgtcataagg attttaagaa gatgtagtct tcttctttca ccaccagata atttacctat
    15661 caaagtatat tgcatagtac cattgaaaag gaatctttca cacatttgag aagctgtaat
    15721 cctagtacca tctgaagttt ctatatagtc actagcttct tttacatagt ctatagccct
    15781 catatcaata ttcatatgag agtcgtcttg agaaaaacaa cctattttaa ccgtttctcc
    15841 aattgaaata tatccactat caggctctat ttctccatta agtatattta taagggtaga
    15901 tttacccata ccatttttgc ctattatacc aattctatct gtacgagtta aagtgtattc
    15961 taaattatct ataacagtat tttcttcaaa tgatttagat atattgtgta tttctattat
    16021 tttatttcca agtctacttg atgcaacaga tatatcaaga ttttcatcag gtttaaaaga
    16081 atctctattt ataagttcat caaatctttg taaacgagct ttttgtttag tgctacgagc
    16141 ctgagctcct cgtcttaccc acgcaagctc tgttcttatt aaattttgtc ttttatcttc
    16201 aatacttgct tctagcgaaa gtctttccat tttcttttcc aaaaatattg aataattccc
    16261 atcataactg aataatctac ccttgtccag ttcaataatt ctatttgtaa cacggtctaa
    16321 aaagtatcta tcatgagtta tcataagcaa tgaacctttt ctggaattaa gatattcctc
    16381 aagccaatct atagtatcat tgtctagatg gtttgtaggc tcatctagta tcaaaaactc
    16441 acaaggtgta ataagggctg atgccagaga gactctcttt ctttgcccac cagaaagttc
    16501 tgctattttt tgattaaagt catttatacc aagctttgta agtactgact tagcttcact
    16561 ttctaaatcc caaagattta aggcatctat tttttcgtgt actgataata attttttatt
    16621 taattcctct gtaaagtttg aattaagttt ttctaggatt tcttgatatt caccaagtag
    16681 ctttaattct tcagaagttc cattaaatac ttgttcaagt acagttgaat tttcattgta
    16741 agttggattt tgaggtagat attctatcct aataccatta gtttttgtta tattaccagt
    16801 ctcactttct tcaactcctg caataatttt gagtaaggta gatttaccag tgccatttac
    16861 accaataagt cctatttttt ccccttcatt tattccaaga gatatatttt ctagtaattt
    16921 tttttcagaa tagcttttac ttatattttc caatgtcatt aaattcatat aattcacctt
    16981 tatttctaat ctattatttt tcaaaatgtt gataatctgg gtttttccaa tagccacccc
    17041 aactccaacc tctgcttaca aaagcattat aacaatcatc accttttttt atcattccaa
    17101 attttgtgct agacctatca gcataaactg tacttacttt aggtgtaaca tcatttccag
    17161 aaacttgagg attctgtaat gggtttatat ctatagcacg tcctttacca tgatttgaaa
    17221 ctacatttgt accagctata gttctataac aaaatgatga ggtattatta tcagacatag
    17281 atttttcatc tacagcatca tagtcatcta ttaacttaat tttttcaatt ggatattttt
    17341 tttcgtataa ttccttaaaa atatccacta cttctggagc tagttcactg tttactatca
    17401 tttctccttg atgagttttt tcatcaaatc cataatatgt aagttttaaa taagatagac
    17461 tatcaaagct tataggttca tcagtaggca tagatttacc aatcatttta ttttttatat
    17521 tatctggtat tgaagaatat gaaaaagtag gcttgatatt attatcttcc tgctttttgg
    17581 tagagctttg attattttta gaatcatctg ttgtattatt tttattttgc atagtttctt
    17641 gactctttat attacctttt ttgttgggat tagagaacag tatctcatat atagataata
    17701 aagcaagaaa taatacagtt attacaatcg ttaattttaa tttattaatt tttcttcgtt
    17761 ttgacttttt actcatatta tcccccagta ttagattcat ataaactata tatattatac
    17821 ctcataaaaa taaactaggc taacaatatt tagttaacct agaacatttc atttatagtt
    17881 tctccaatta tttttattcc aatttcaatg tcttcaaaag acaacctaga aaatccgagt
    17941 cttaaagtat caaaaccaga gttatctgta aaaaataagt ctccaggcat aaatataaca
    18001 tttttttcat aacatttttc taataaaact ctagtatcta tattttttag ttttataaat
    18061 atatgcaaac caccatcacc caagatatat tctgttttta tatatttttt tatacattta
    18121 tatgcaaagt taaatttgtc tccatagaat ttacgtattt ttttaatgta tttttcaaaa
    18181 cctccattac ttaaataatc aaataaaatt ccttggtcta aaaaggatac atgtatgttt
    18241 ctgcaccttt ttacactttc tagcttattg attacttttt tatctgcaaa tatccatcca
    18301 attctcattc caggaaataa aatctttgaa aaactaccaa tatatacgac cccattattc
    18361 atattatcta gggaacagat aggaaatata tgagaactat tgtagagtaa ttcttcatta
    18421 aatccatctt ctatagttgc tatattgtat tttttcataa gattatagaa tttgtaccta
    18481 ttttcaggag tcattacaat tcctgtagga ttgtgataag agggtgtaat gtatgcaaac
    18541 tttattttat ttttatactt aactaaattg tcttctagca tattgaagtc caaaccatct
    18601 tcatttatat ctacacctaa gatattgagg ttatgagatt tcataatttt tatagcagta
    18661 ttatgagtag gattttcaca tattatgtag tctttttctt gggtaaaaga tgacataata
    18721 atatcaaagc cttctgtgaa tccatttgtt attagtatat ctttattact tatatcagca
    18781 cccttgtttg tcatatattc tagtaaatag tcaataagtg gcttataacc ttgtgcatat
    18841 ccatagttga gtaatttatg tccttctagt gaaattctat ttaaaaagga tttttttaat
    18901 tcttccatgt cgaataaatc tccatctgga gaaatacttt taaaggaaat caggtcagag
    18961 ctccaaggaa tttcactttt tataatgtcc aattcattgg cttttttaga gtatgtattc
    19021 tctaaacaat cccagtttaa tgaccatgta gtatttgaag acttattctt tgatttaaca
    19081 aatgtacctt ttcctgaaat agagtagatt aatccttctg attttaattc ttcataagtt
    19141 aaaactacag aatttcggct aacttgtaaa agctgactaa gctcccttgt agaagggagc
    19201 ttactattgt ctggaattag tccttttgat atcatatcac ttatataatt tttaagttgt
    19261 atataaattg gttcattatc atttaatttt agatttgaaa atatcaaatt accacctcaa
    19321 attatttcat ctaatttaaa gagtattcta taagtttatc taatagttca ctgtacttaa
    19381 tacctctagc agcagcactt ctaggaatta aactagtttg agtcattcca ggaagagtat
    19441 ttagttcaag aacatatgga ataccttcac ttataatcat atcaactctt acatatgctt
    19501 tgcaattaaa aattttccaa caagtctcag aaatttcgtt tactcttttt tgaagttctt
    19561 catctaagta aacaacttct tcctttgcac ctttttcaac tgagtattta gcttcataat
    19621 caaagaatcc tgaatcagat tttatagata ttgtagggaa tacttctcca tttaatatga
    19681 atgaagtata ttctccacct ggtatatatt tttcaatcat tacaaattca tcaacttcca
    19741 gaccttttct aactgcttct tcaacttcat ccttagaatg aataaagaat gtggctacac
    19801 ttgaacctcc tgagtttggt tttataaata ctggataacc tatattatct atttcatcat
    19861 agtctatttc atcaactgat tttactaaaa cccatggagc agtaggtagg tttgaatctc
    19921 taagcatttt tttagttata tttttatcca tacacatccc actgcaaaga ggtccacatc
    19981 cagagtatgg tatatccata gtctcaagta ttgattgtat acatccatct tcaccaaatt
    20041 ttccatgtaa agcaagtatt gcaaaatcta tatcttctgg tatctttgta aatatatctt
    20101 ttttatcatc tattattact tttacaactt catatttatt tttatcaagg ttattatata
    20161 tctcttttcc tgaatttaaa gaaacttctc tctctgatga tattccaccc attataactg
    20221 ctattttcat attatacact cctattttaa tttttattat tttaaagtaa actttttatt
    20281 tacatactta attatatgaa ttaacagttg aggatgaaag taccactata tacgaagttt
    20341 atattatgtg tggttttaga ataataatct agtttaaaga tatgtttaaa tgatttttta
    20401 actttattta tgttattatt tttgctaaaa ttattgttat gaacttaaga ctagtcttaa
    20461 tggctataaa tcaaggagtg ataaattttg aaagctgaaa taataagtgt aggaacagaa
    20521 atattattgg gagatatagt aaatacaaat tctcaatttt tagctaaaga acttgcatca
    20581 ttgggaatag aagtatacca tcagagtact gttggagata acaaacaaag acttttagaa
    20641 tgttttgatg aaagcttaaa gagaagtgat tttgtaatta ctacaggtgg tcttgggcca
    20701 actggtgatg atatgacaaa agaaactgct gcagaatact ttggtcagaa attagaacta
    20761 cataaacctt cactagaggt tttggaatct ttctttgtta aaacagggaa aaaaatggcc
    20821 gaaaataata tgaaacaagt gtactttcca aaagatgcta tagtactaaa aaataataat
    20881 ggtacagctc caggggcaat tttaaaaaaa gatggtaaat ttataatagt attgcctgga
    20941 cctccaagag aaatgaaagc tatgtttaat gagagtgtca aaccttactt acaacaattc
    21001 acaaatgaaa tgctagtatc taagacttta agattatatg gaataggtga gtcaaatcta
    21061 gaattagaaa ttctcgatat aattaatgag cagactaatc caacagttgc tttatatgca
    21121 aaagaattag aagttacaat aagaataact gcaaaggctg aaaatgaaag agaagctttt
    21181 aaattaataa aaccagtaga agaaaaaata aaaagtagag ttggtaaata tgtatatact
    21241 gaaggagata tttccatatc tgaaggggag actgctttag aagatgctgt ttctaagcta
    21301 cttgtagaaa aaaatctaac aatagcagtg gctgaatctt gtactggagg tttggtttct
    21361 tcatctctta taaactatcc aggtatatcc tctgtatttc ttgaaggttg tgtaacatat
    21421 tctaatgatt ctaaaatgaa aagattagga gtaaaaagag aaactttgga agagtttgga
    21481 gcagtaagtg agcaaacagc tatagaaatg gctgagggcg ttgctaaagg acttaaagct
    21541 aatataggta tatctacaac tggagtagct ggacctggtg gaggaaccaa agagaagcct
    21601 gtgggactgg tttatactgc aatatatata aatggaaaaa caatagtaaa gaaaaatata
    21661 tttaatgggg atagaagaaa gataagatta agagctacta gagatttgct taatgagttg
    21721 agaatacaat tagaaaagct ttaagttatt ttgattaaag tattagtaaa atataaaatt
    21781 gtttttggag gcttttaatt tatgataaaa catatatttt gtgatttaga tggaactcta
    21841 tatgaaaatg gaaccataac aaaagaagat atagtagcaa ttgaagaaat agaaaaaaag
    21901 ggagtacagt ttaatgttgc tacaggtaga atttttaaac aagcacataa tattatcaaa
    21961 gatagtttag atatgaatgg atattatgta tgtgaaaatg gttcatttat atatgataaa
    22021 gattataatg taatttttaa aagaacgata gatgataatt tagtaaaaaa agtcattgat
    22081 agatttgagt caagtgatgc acaactttat tttaaatata agggtgatgt aattgtgggt
    22141 agtgatacca ctgcttttag acattattct agtgacttta tagttgaccc agattttgaa
    22201 aaaagaagta gttttgataa tcttatagga aatataggcg tatgttctga aaatttggaa
    22261 gagttatcca gaatagaact ttatctaaaa agtgagttta gtgaagtctt agaaatatat
    22321 ttttctggaa catatacttt aaatatagtt cctaaaagtg tgtcaaaaag aggaagtata
    22381 gagcatgtaa taaaaacgtt aaatgtaagt ccagatgaag ttgctactat tggggattct
    22441 ccaaatgata tttgcatgtt agaaggattt aaatatagtt ttgcaatgtc taaggcaagg
    22501 gaagatgtaa agcaaagtgc aaattatgta gtagatagtg taaaaagtgc catagatgtt
    22561 ataatggaga taaatagtta gtataaaaaa tgactgtcct agagaagtat aaaattatat
    22621 tctatagagc agtcatttta attatattca attataagtt tatataagtt taaaaattct
    22681 taagttaacc gcaatttgct atatgatatg attttattgt attagataaa atagagtcaa
    22741 gtatataatt tatgacacta ttacaagtat aagctgaaaa ttttaaatca ttcatataat
    22801 cagttttctt tttgtaatca ctataaagtt tagtaaaaaa cttctcaaaa ctactattta
    22861 tagtaccaca tttaatttta gataaatcaa catcttttgc aaatgctcca agttctcttt
    22921 cataagtaac atgtttattt aagctatgct taaactccca acgttcactg tgagctacac
    22981 agaaaaaatc acataaaaaa tgacatatga ctcctagttc ctgactaagt ctacttactg
    23041 aaaagtattt agaaagacta tctatattaa gactgcatag atagtttatt ttagaaacaa
    23101 tcatattaaa tgactcatct aaataatgtt tttttaattt atatttagaa aaagcatcag
    23161 gctttatatt gccatatata aaattttttt cgcttataaa gaatgtttta ttttcatcta
    23221 tattatcaag tattgatttt gccataataa aatgggtatt cattaacata tttacacctc
    23281 gtatagttta ttagacttat taaatatatt attatgatat gatatttatg ataaaagttt
    23341 aatggtaaat atatgaaact aagtcaacag tatttattta aataatttca accatattca
    23401 taataacagt aaatggccaa atttacaata tgtaaatagt gttaagtttt tatttatcaa
    23461 atttaaaatt aagtattagt atactttcta attaacctat tggctttaga ctgtgagatt
    23521 tcaaggtgtt tggctacttt ataagaacta ccaaattttt cataagcatc caaaattata
    23581 tttttttcat actctttaat taaagtgtca aaatttttat ttttataatt agtagatttt
    23641 gagtcagata taataacctt attaatttgg gatatatgtg ttttatttcc attattggta
    23701 atatgtatat tttttgtaag aatgtttggt aagtcatcta ttgttatatg attatctgta
    23761 caagtaagca agattttttg aattatattt tgtaattctc ttatattgcc tgggaatcta
    23821 taatttgcaa tagcatccat agcttcttta gaaatagttt tatttagatt gaagtcactt
    23881 gagtacctat taaaataata ttttataatt tccactagat tatcccttct ttctctaaga
    23941 ggaggcaatt ttatttctat aacatttaat ctatagtata aatcttctct aaactttttt
    24001 tcttttactt ttgaaactaa atcaacattt gtagcagata taattcttat atcgaccttt
    24061 ttatttttta agctcccaac tggagtaaaa gttctatcct gtattaattg caaaaatttt
    24121 gcctgtagat tttgtggtat atctcctatt tcatctaaaa aaagagttcc tccattagct
    24181 aattctacga gcccaacttt acctttagta cttgcacctg taaaagcacc tgaagtatat
    24241 ccaaaaagtt cagattcaag aagttgtgga gatatagttg cacaatttat tgttataaat
    24301 gggccattag atctattgct gttgtggtgt atatatctag caagtgagct ttttcctgtt
    24361 ccagattcac ccaataaaag tacacttaca tccgttttag aaacatgatt tactgtgttt
    24421 atgattggtc tcataactat tccgtccata attacagtat cctcaattct aagtgcagtt
    24481 ctgtatagtg tttctacttc agatttatat ttttttactt gcttttttgt gtcttcaagt
    24541 ttatttttta tattattaag ttcagttata tctctacaat tttcaacagt aaattctaag
    24601 tttccatttt catcaaatat aggggtagca gttatgataa gctttttccc tgtttgagta
    24661 tcttgttcca gtgaaaattt agtctttgtt tttaatacaa ttggtatggg gctttttgtt
    24721 gaatatccag agtcttctaa aaaagaaaca ttttttccta aaatatcttc tttttttaga
    24781 ccataatttt cttcaaatgc tttattacaa tatattatta ttcctttatc atcagaaaca
    24841 catatttcat catgggaagc ttctaatatt ttttgataaa attccaaatc taaattcatt
    24901 tttatatttt cctcctaaat ttttaatgat atattaaaat ag
//
LOCUS       DAKBNN010000038        24763 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000038 DAKBNN010000000
VERSION     DAKBNN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.038"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            98..3934
                     /locus_tag="OUB02_001344"
     CDS             98..3934
                     /locus_tag="OUB02_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HCT8885113.1"
                     /translation="MKKKLKASKRILSFIMVFVTIITTILTSSVSVYASELDMSEHTG
                     FNYLGISPITGRKINHNIYRMKMDNKVVFCVEPGIKTASGGGYISEEYINSKKDILSK
                     IAYYGYTDTNKSKYDYALTQIIIWEELGDKFISTTLPNYHKRKAEILEKVNKHNILPS
                     WNNEKVAVKSGETIELKDKNGIIREMKLESNNTGTNIVLEENTLKITANEKSTSGVIS
                     YRKIPENEVGTSIVYRKPNYQSLAEFHMESSMSANLNINVIKLGNIKVKKIDEDTGKA
                     LANAKLRFEYDGKSKEIITDSNGIANIEDIPEGTTVTISEVTAPNGYFNKGEIKKVVI
                     EANKTIEVTLGNKEQLGKVILSKSGKDFGVEMPNDSYKLEGAIYGIYNENDEKITNIT
                     TDKFGKATSEKIKLGKYYALEEKAPEGYLINKEKIPFELKYDGQTVEVTSTAISHKDT
                     EQKGKAILIKEDEKTGSIPQGSAKLDGAVYELRKSNNDELVETVTIKAGKAKVENLYL
                     DKYYWIEKKAPEGYLLDTTKHYFEISYAGENVETAIKETLVKEKVITGGFDLIKFGEY
                     DWKEKIKNIFKTKNKEIKPLKDVEFSVFSDTTGKLVKTGYTDEKGYLKFEGLPYDTYT
                     VKETKTPEGYIAAKDFKVIVKKQNETHHYAIQNKVIEEKLKVVKIDSETKKIIPVKGA
                     EFKIKSLQTGDFITMSKNNEDGKTDTFYTNDEGYLITPEVLSYGEYELTEVKAPDGYV
                     LAKGPIHFKVDGSSNGVIEIRFENTSQKGIVEFTKKGQVPKDIKIEETKYGKLHEIIY
                     EYKPLENVTYDIIAKEDIITNDGTIRFKKGDVIETLTTDEKGKWKSSELYLGKYQAIE
                     KSAPNGYIISSDPIDFELSYSGQLVELTKTSITANNDFQSLLVNIFKNEEKIEGWKDN
                     KPIIKDVKGNGKIFGIFIREEKEISNNIKIPENSMVGIKDVKDGVAELNIKLPEGKYY
                     LKEIDSGENHILNEKEYDFEFNSNNNDKEVKINIYADGVDFGDKNENIKPILNKLQLN
                     SFKIKKINEKAIQNKRNGFSFEFTELGQGAKFILEDKKGDIIQEVTIGKDSIGEFNNI
                     PVGTFYLKEKETSNDKYILSNKVIRIESTKKGIKAFDDSNNLISEKNNSEDKILLEIK
                     NELIKGGAKLIKTDLVTGEALPNTGIRVLNEDKNILIEGKTDNKGVFYFNNLPKGIYY
                     FQEYEAPKGYQIDETPMKFEIKENGEVVTCKMTNKKFEKVSIPKTGDDNSVILYGIGM
                     LASGTLLLKMRRKKDK"
     gene            4098..4412
                     /locus_tag="OUB02_001345"
     CDS             4098..4412
                     /locus_tag="OUB02_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT8885114.1"
                     /translation="MKLKHIIPNMEKTFGNLEYAGEGDVEQKRINGKMTVMSRSYNLY
                     SDIQRADDIVVVLPANAGEKSFEVEERVKLINPKIIAEGYKIGTRGFTNYILLADDIV
                     KA"
     gene            4426..4803
                     /locus_tag="OUB02_001346"
     CDS             4426..4803
                     /locus_tag="OUB02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCT8885115.1"
                     /translation="MRLANGIVIDKEKTFGMLKFSALRREVRVQNDDGTPSNEIKERT
                     YDLKSRGQGRMIQVSIPASIPLKEFDYNAEVDIINPVADTVATANFNGTDVDWYIKAD
                     DIVLKNRQEQLGGDLQKDIHKKK"
     gene            4830..6233
                     /locus_tag="OUB02_001347"
     CDS             4830..6233
                     /locus_tag="OUB02_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCT8885116.1"
                     /translation="MKFYKYKGNRIRASDKSLVFHFSISILSIIFITLLIVLNVKQIA
                     EINWKNINLFSENGLSFSLTTYNILTIILAISICLLVAFLYYHYQYDRFKKLIHRQKL
                     ARMILENKWYESESIKDSGFFKDLNSNKQKEKIVYFPKMYYELKDGLIYIKVEITLGK
                     YQEQLLNLEKKLESGLYCELISKELKDSYIEYVLLYDTIANRISIDEVKVSNGSLKLM
                     KSVYWEFDKLPHMLIAGGTGGGKTYFILTIIEALLRTDSILYVLDPKNADLADLKTVM
                     PNVYYRKEDMISCINSFYDEMMKRSEVMKTMDNYKTGENYAYLGLPANFLIFDEYVAF
                     MEMLGTKENATVLNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGR
                     MSELGYNMMFGEANKEFFLKQIKGRGYVDVGTNVISEFYTPLVPKGHDFLKEIDKIIK
                     SRQVEQSACEAKDDCLN"
     gene            6423..7607
                     /locus_tag="OUB02_001348"
     CDS             6423..7607
                     /locus_tag="OUB02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCT8885117.1"
                     /translation="MNIEKWVLDLKEKRIEYGVSQNKLATSVGITREYLNKIENGKII
                     VKNEIKEKLIESMKRLNPDSPLTMMIDYIRIRFPTTDVKHVVNDILKLKIEYMIHEDY
                     GFYSYSEHYVIGNIFVLVSQDIEKGVLIELKGQGCRQFENFLLAQHRSWYDFLLDALI
                     DGGVMKRLDLAINDMVGILDIKELTDKCNNEECISVFRSFKSYRSGELVRKNEKIGMG
                     NTLYIGSLKSEVYFCIYEKDYEQYIKLDIPIDEAKIKNRFEIRLKNERAYYAAVDLLA
                     NRDAEKTAFSIINRYIRFVDKNKDKNRSEWKINEHWNYFIGEGREKLKLTTSPEPYNI
                     YKTLNWLSRQVAPTLKMIKRLDGLNNTTLIEDLIRHAELTDRHKKIIHQNSVNVEDII
                     CE"
     gene            7674..7895
                     /locus_tag="OUB02_001349"
     CDS             7674..7895
                     /locus_tag="OUB02_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885118.1"
                     /translation="MNFGQNLYNWFLANAQSLVLMAIVIIGVYLGFKREFSKLIGFLV
                     VALIAVGLVFNAAGVKDILLKLFNKIIGA"
     gene            8011..8277
                     /locus_tag="OUB02_001350"
     CDS             8011..8277
                     /locus_tag="OUB02_001350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885119.1"
                     /translation="MEWKRYKNFISLIVTILIVIWLVLKFIFKNQFSDFISAIIMTII
                     GITYFSTYIKSYRKNKNRFEILFILLSFIMSISGVLLVIGFFVN"
     gene            8372..8875
                     /locus_tag="OUB02_001351"
     CDS             8372..8875
                     /locus_tag="OUB02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCT8885120.1"
                     /translation="MEEMRVYIANLGKYNEGELVGEWFTPPIDWDDLKERIGLNSKYE
                     EYAIHDWELPFEISEYTSIEEINRLCSMAEELASTDIGEAISDIQKAFFSSFEELYEH
                     KDDIIYYPGCSDMSDVAYYLIDDCNMLGEIPENVRNYINYEAYARDLEINYSFVVTNH
                     GVFEYAN"
     gene            8955..9371
                     /locus_tag="OUB02_001352"
     CDS             8955..9371
                     /locus_tag="OUB02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT8885121.1"
                     /translation="MKKIKSYTGIWNVEKVIYAINDFQLPFPITFTQITWFILTLFII
                     ILFGKLPPLSMIEGVFLKYLGIPVFITWFMSQKTFDGKKPYGFLKSIILYAFRPKITY
                     AGKRIKFKNETIDENITIVRSELYVPDKIYREQSCI"
     gene            9331..11778
                     /locus_tag="OUB02_001353"
     CDS             9331..11778
                     /locus_tag="OUB02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8885122.1"
                     /translation="MFPIKYIENNLVFNKDGECFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQNRNGKIHALQIATESSIRATQERSKKNITGKLKEIAYEKIDEQTEALVSMI
                     GDNQIDYRFFIGFKLLVTEEDMNIKNIKKSILANLSDFIYDVNNKLMGDFVSINNDEI
                     MRFSKMEKLLENKISRRFKVRRLDKNDFGYLIEHNYGKKGTAFEDYQYHLPIKKLKRE
                     TLVKKYDLIKPTRCLIEEKQRYLKITHEDSESFVAYFTIESISGEMDFPSSEIFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALTTVRNKKKELRDLDNHAWQAGNETGTNVVEALDAVN
                     ELENNLDQTKESMYKLSYVIRVSADSIDELKRRCDEVKDFYDDLNVKLVRPMGDMIGF
                     HNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQQLGENEGIYIGYSLDTGRNVYLKPY
                     LASQGVKGSVTNALAAAFIGSLGGGKSFANNLLVYYSVLFGGQAVLVDPKAERGKWKE
                     NLPEIANEINIVNLTSDIENKGMLDPYVIMKEVKDSESLAIDILTFLTGISSRDGEKF
                     PILRKAIRNVTLSKCRGLLKVIDELRKDNSQISNNIADHIESFTDYDFAQLLFSDGNI
                     KGSINLEKQMNIIQVTDLVLPDKDTSFEEYTTMESLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWTFLNVAQGKTLSNKLVRAGRAMNAGVYFVTQNAGDVSDENLKNNIGLK
                     FAFRSTDINEIKRTLEFFGVDKEDDNNQKRLRELENGQCLIQDLYGRVGVIQIHPVFQ
                     ELLTAFDTRPPIRKEVD"
     gene            11778..13997
                     /locus_tag="OUB02_001354"
     CDS             11778..13997
                     /locus_tag="OUB02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT8885123.1"
                     /translation="MKSKSFRNLSWITVLIVILVLFFIYISESVVYAEGLVDETINSN
                     NIYSMYPLRNYQLDFYVDNSWVWLPWNWTDGIGKSVQYGLYCITNFIWIISLYLSNAT
                     GYVVQEAYKLDFINDMADSISKSMQTIAGINQNGISNSGFYIGFLLLFVLILGIYVAY
                     TGLIKKETSKAINSVINFVVIFIISASFIAYAPDYIKKVNDFSSDISTAVLNLGTKIV
                     TSNSNNEGKDSIKLIRDNLFSIQVQQPWILLQYGNSNIEEIGKERVEKLLSTDPSEDD
                     GKKREEIVKTEIEEKHNNNLTITEVMSRLGKVFFLFIFNIGITIFVFLLVSMMIFSQI
                     LFIILAIFLPLSFLLSMIPSYESIGRQAIVKLFNTIMMRAGITLIITIAFSISTMFYT
                     ISTDYPFFMIAFLQVVTFAGIYMKLGDIMGMFSLHSGDSQNMSSRMFRRPYRTMRNKS
                     KNIERNIRKALISRGKDKERLSSNINNPQRNVNNTQKRTGYPHSRENYDKSVSKESFS
                     KRIGNTVGAFMDTKKNISDKSKQVKENIQDIPVQAKYAVHNTKEKAKDNISDFKRGIV
                     EEKEKRQQDRNKKQQNHRETIAQKRAKLGNIVEAKEKNSTDNKNIDGYSDSKYKPSIN
                     SERVKKKKGENIRPTVSKKIYDEKDREKSNSINKIKRSTEKPIHKERDIVNQSEELSN
                     KVENNQSVKVKNSKTMKIERHNINSNLKGPKYNHIDIKERKIKNKSNFTPENKVRKQ"
     gene            13994..14992
                     /locus_tag="OUB02_001355"
     CDS             13994..14992
                     /locus_tag="OUB02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCT8885124.1"
                     /translation="MKIKLITIFISIFLFIFGVILMFVPLISDEDHNGGMNQNIIGMN
                     LSPEVLKFKPMVERYAKKYDVQEYINYLLAIMQVESGGTLVDVMQSSESLGLPPNTLG
                     TEESIEQGCRYFSNLLKTSKEKGCDTNSTIQAYNYGFGYLNYIKSNGKIHTYSLAENF
                     AKNMAQGVRVTYTNPIAVAKNGGWRYKYGNQFYVELVLQYVGTSEFNDETVKIIINEA
                     LKYKGWKYVFGGDNPNTSFDCSGLTQWCFKKAGINLPRTAQQQYDIMQHIALDQAKPG
                     DLVFFHGTYNAGTYVTHVGIYVGNNKMYHAGDPIVYEDLTDSYWQKHLIGAGRIKK"
     gene            15006..15899
                     /locus_tag="OUB02_001356"
     CDS             15006..15899
                     /locus_tag="OUB02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT8885125.1"
                     /translation="MLRKKDKKHKEKKILVIRGGTNKKFVIALWIILIVSITFSVYKN
                     FTAIDKHTIHEKKIIEQKIIDTNGIENFVKNFAKEYYSWENNKESIERRTAKINEYLT
                     KELQELNIDTVRSDIPTNSSVKDVQIWSVNQIKENEFEVKYSVEQTIKEGEEIKDITS
                     SYEVVVKIDNTGNMIIIKNPTLSKMSEKLNYESKKEETDSTIEQKTIKEITEFLNTFF
                     KLYPTASKNELEYYVKGDALKPINGDYIFSELVNPIFKKEWKNIKVLTGVKYIDQQTK
                     MIQVSQYELVLEKDNNWIIVK"
     gene            16155..17618
                     /locus_tag="OUB02_001357"
     CDS             16155..17618
                     /locus_tag="OUB02_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCT8885126.1"
                     /translation="MITGELKSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDDV
                     ETKNEAEAVLLGFEPERIFSDNEQELRWSKFSNEGDAQKMYDIVQNKVFPFIKNLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGINGIEFKKENDTKGDLYEYLLSKLSSAGTN
                     GQFRTPRHIIDMIVKLMKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHNDLFYVQGLKE
                     HFNNTMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKEKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRILKTGGRCASIVPDGVLFGSTKGHK
                     DIRKEIVENHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGSGGTDKVWFYDMKADGFS
                     LDDKRNPVEENDINDIIERFSNLENEEDRKRTEQSFFVPVEEIRENGYDLSINKYKEI
                     EYEEVVYDAPSVILGRIKELEKEITKGLEELEGMLEV"
     gene            17620..18855
                     /locus_tag="OUB02_001358"
     CDS             17620..18855
                     /locus_tag="OUB02_001358"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCT8885127.1"
                     /translation="MKYIKLKDICDLQNGFAFKSSDYIDSSTVLNCRMSNIRPNAKFD
                     IMYNPKYLPENFVERYKEYILNDGDLIIAMTDLANDPKILGVPTIVETKGNTLLLNQR
                     VGKLIILDDKLVDKYYLKYSLSLPQHKPYYKKFAGGGLQINIGKEQILNLKIPISNIE
                     IQRKVVEILDKSQELIDKRKKQIEELDLLIKSKFIEMFGDPINNPMKWEVKKMKDISV
                     NIMSGNTPKGGEQVYVESGIMFLRSQNVWKNKLLLDDVAYIDQATHNKMKKSSLKNKD
                     ILMTKTGRINTENSSLGRAAMFLGEDDSANINGHVYLIRLQEQIINEFVLYILTSKEY
                     RGYIRSVCVGGIDKRQLNKEHIEDFPIIFPPIEKQKKFVSYVNQVDKLKFKMENSLKE
                     LEDNFNSLMQKAFKGELFN"
     gene            18881..19186
                     /locus_tag="OUB02_001359"
                     /pseudo
     CDS             18881..19186
                     /locus_tag="OUB02_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19207..19569
                     /locus_tag="OUB02_001360"
     CDS             19207..19569
                     /locus_tag="OUB02_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885128.1"
                     /translation="MDNNMRNEIESWGIPNIDELKGRNIKFVFNSSEINKEKGSFVEL
                     HCEKESVKFCLYDFTNDIEIFTMEFFARPKSLLMLTNELPSVKLELLNVNDFNLRRKG
                     IASYYLDRFKEYAIKHKY"
     gene            19838..23170
                     /locus_tag="OUB02_001361"
     CDS             19838..23170
                     /locus_tag="OUB02_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCT8885129.1"
                     /translation="MGRNFEFLKGKKQFDGFRAQCFEAEKSIIVSPATCAILSRRALE
                     LAVKWVYGNDSELVLPYQDSISSLIHNESFRELIDSSMFNILKYIIKLGNVAVHTNKP
                     ISREEAILSLHNLHQFVSWIDYCYADNYTATNFDESLLHEGDNKRTRPEELNNLYEKL
                     SSKDRKLEEVIDENKEKRKLGTEKRKENTANNTFKVDKISEAETRKRYIDVELKLSGW
                     TFGKDIQIEVEVNGMPNESGLGYVDYVLYGDNGKPLAVVEAKRTSVDEKKGQQQAKLY
                     ADCLEKQYNQRPVIFLTNGFRYYIWDDCGDREVFGFYKKSELQLMIDRRTSKKSLKDI
                     KISDEISNRYYQKEAITSVAEAFERNQRTALLVCATGTGKTRTAISIVDVLSRHNWVK
                     NVLFLADRKALVKQAKKNFSKLLPNLSLCDLLDSRDNPEESRMVFSTYPTMMNAIDDT
                     KSKDGDRLFTPGHFDLIIIDESHRSIYKKYKSVFDYFDAYLIGLTATPRDEIDKNTYD
                     IFNLEKGVPTYAYEYEKAVEDRYLVDYRTIEIKSKILEGGIKYDDLSQEEKEEYEMMF
                     DDDENIGDEISSSAINQWLFNTDTIDLVLNKLMTDGLKIEGGEKLGKTIIFAKNSRHA
                     KSIVERFNKLYPEYGGTFARVVEHKTPYVENIIDDFSDKYKMPQIAVSVDMLDTGIDI
                     PEILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGIGFDKEEFLIFDFCNNFEFFRVNP
                     KGFEGNKVESLTERLFNVKVDIVKELQDIKYQDDEKYVNYRNELLGELIKSVQALNDE
                     SYLVRMNIGVVHKYKDEKAWNAIGAIEQNEIKKHISPIIKSINDDELAKRFDLLIYVI
                     QLANLQNIVFKKGIKSVMETADKLSKLGTIPEIKEKKAIIERIVEPEFWECADIFDME
                     NVREALRDLIKYLEKSTQGIYYTEFSDSIISEESHSSMYNVNDLKSYRKKVEFYLKEH
                     KDELAIYKLRNNKKLTEQDIQTLEKVLFNELGSKEEYEKEYGKTPVTQLVRKILGLDR
                     NAANEAFSEFLNNKSFNSSQIHFVKLIVDYIVKNGFIEDNKILTEDPFRSVGSIIDLF
                     PNNVSERAKLIKTINEIRENTL"
     gene            23298..24263
                     /locus_tag="OUB02_001362"
                     /pseudo
     CDS             23298..24263
                     /locus_tag="OUB02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084758930.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT        10372 a         2346 c         4381 g         7664 t
ORIGIN      
        1 aataaactag ttattaacaa tagaaataga tattgaattt atatattcga tgtctatttt
       61 tttatataaa aatatattta agaaaggatg atagttaatg aagaaaaagt taaaagcatc
      121 taaaagaatt ttaagtttta ttatggtttt tgtaactata attactacta tacttacatc
      181 atcggtttca gtttatgcta gtgagctgga tatgagtgaa catacaggat ttaattattt
      241 aggtattagt ccaattacag gaagaaaaat taatcataat atttatcgta tgaaaatgga
      301 taataaagtt gtattttgtg tagaaccagg aattaaaaca gcaagtggag gcggatatat
      361 atcagaggag tatataaatt caaagaaaga tatattatct aagatagctt actatggata
      421 tacagataca aataaatcaa aatatgacta tgcattaact caaataatta tatgggagga
      481 gttaggagat aaatttatat ctacaacact tcccaattat cataaaagaa aagctgaaat
      541 acttgaaaag gtaaataagc ataatatatt accttcatgg aataatgaaa aagttgcagt
      601 taaaagtgga gaaacaattg agttaaaaga taagaatggc attattagag aaatgaaact
      661 agaatcaaat aatacgggaa caaatatagt acttgaagaa aatactttga aaattacagc
      721 aaatgaaaaa tccacttctg gagttattag ctatagaaaa attcctgaaa atgaggttgg
      781 gacatcaata gtctatcgta agcctaacta tcaaagctta gcagaatttc atatggaaag
      841 ttcaatgagt gcaaatttaa atataaatgt aataaagtta ggaaatatta aagtaaaaaa
      901 gattgatgaa gacacaggaa aggcattagc aaatgcaaaa cttagatttg agtatgatgg
      961 taagagtaaa gaaattataa cggattctaa tggaattgcg aatatagaag atataccaga
     1021 aggaacaact gtaacaatat cagaagtgac agcaccaaat ggatatttta ataaaggtga
     1081 aattaaaaaa gttgttattg aagcaaataa aactatagaa gttacattag gaaataaaga
     1141 acagttaggt aaagtaatac ttagtaagag tggaaaagat tttggagtag aaatgcctaa
     1201 tgatagttat aaattagaag gagcaattta tggaatctat aatgaaaatg acgaaaagat
     1261 aactaatata accacagata aattcgggaa ggcaacatca gaaaagataa agcttggaaa
     1321 gtattatgca cttgaagaaa aggctccaga gggatattta ataaataaag aaaaaatacc
     1381 atttgaatta aaatatgatg gacaaactgt agaagtaaca tcaacagcta ttagtcataa
     1441 agatacagaa caaaaaggaa aagcaatatt aattaaagaa gatgaaaaaa cgggatcaat
     1501 acctcaagga agtgcaaaac ttgatggtgc agtttatgaa ttaagaaaat caaataatga
     1561 tgagttagtt gaaacagtaa cgattaaagc tggaaaagca aaagtagaaa atttatatct
     1621 tgacaagtat tattggattg aaaagaaagc tccagaagga tatttattag atactactaa
     1681 acattatttt gaaataagtt acgcaggtga aaatgtagaa acagctataa aagaaacact
     1741 agtaaaagag aaagtaatta cagggggatt tgacttaata aagtttggag aatatgattg
     1801 gaaagaaaaa ataaaaaata tttttaaaac aaaaaataaa gaaataaaac ctttaaagga
     1861 tgtagagttt tctgtattta gtgacacaac agggaagtta gttaaaacag gatatacaga
     1921 tgagaaagga tatttaaaat ttgaaggatt accttacgat acttatacag taaaagaaac
     1981 taaaacacca gaaggatata ttgcagcaaa agattttaaa gtaatagtta aaaaacaaaa
     2041 tgaaacacat cattatgcaa tacagaataa agtaattgaa gaaaaattga aagttgtaaa
     2101 gatagattca gaaactaaga agataatacc agtaaaggga gctgaattta aaataaaaag
     2161 cttacaaact ggagatttta taacaatgtc taaaaataat gaagatggaa aaactgatac
     2221 gttctataca aatgatgaag gatatttaat aacaccagaa gtattgtcat acggagaata
     2281 tgaattaaca gaagttaaag ctccggatgg ttatgtatta gcaaaaggtc ctatacattt
     2341 taaagtagat ggttcatcaa atggggttat tgaaataaga tttgagaata catcacaaaa
     2401 aggtattgtt gaatttacaa agaaaggaca agtacctaaa gatataaaaa tagaagaaac
     2461 taagtatgga aaattacatg agataatata tgaatataaa cctttagaaa atgtaaccta
     2521 tgacattata gcaaaagaag atattattac aaatgatgga actatacgct ttaagaaagg
     2581 tgacgttata gaaaccctta caactgatga aaaaggaaaa tggaaatcat cagaattgta
     2641 tctaggaaaa tatcaagcta ttgaaaagag tgctccaaat ggatacataa tatcctcaga
     2701 tccaatagat tttgaattaa gttattcagg acagttagtt gaattgacta aaactagtat
     2761 tacagcaaat aatgattttc aatctttatt agttaatata ttcaaaaatg aagaaaaaat
     2821 tgaaggatgg aaagataata aacctataat aaaagacgtt aaaggtaatg gtaaaatatt
     2881 tggaatattc attagagaag aaaaagaaat atctaataat attaagattc cagaaaattc
     2941 aatggtagga attaaagatg ttaaagatgg agttgctgaa ttaaatataa aactaccaga
     3001 aggtaaatat tatttaaaag aaatagattc aggagaaaat catattttaa atgagaaaga
     3061 atatgatttt gaatttaact caaacaataa tgataaggaa gtaaaaatta atatttatgc
     3121 agatggtgta gactttggag ataagaatga aaatataaaa ccaatattaa ataaacttca
     3181 attaaatagc tttaaaataa aaaagattaa tgaaaaagct atacagaata aaagaaatgg
     3241 atttagcttt gaatttactg aattagggca aggggctaag tttatattag aagataaaaa
     3301 aggagatata atacaagaag taacaatagg aaaagactca ataggcgaat ttaataatat
     3361 accggtagga acattttatt taaaagaaaa agaaacatct aatgataagt atatattatc
     3421 taataaagtc atacgtattg aatcaacaaa aaaaggaata aaggcttttg atgatagcaa
     3481 taatttaatt tcagaaaaaa ataatagtga agataaaatt ttgctggaga ttaaaaatga
     3541 attaatcaaa ggaggagcta agttaatcaa gactgactta gtaacagggg aagctttacc
     3601 taatacagga attagagtat taaatgaaga taaaaatatt ttaatagaag gtaaaacaga
     3661 taataaaggt gtattttatt ttaataattt acctaaggga atatattact tccaagagta
     3721 tgaagcacct aaaggctatc aaattgatga aacaccaatg aaatttgaaa taaaggaaaa
     3781 tggtgaagtt gtaacttgca aaatgactaa taagaaattt gagaaagttt ctatacctaa
     3841 aacaggagat gacaatagtg ttattttata tggaatagga atgttagcat caggaacatt
     3901 attattaaaa atgagacgta aaaaagataa atagaagtat gtaaatgtca gatataaata
     3961 agtatctgtt tttttataca aatttttatg aatattggag gtatcatgaa ttataaagta
     4021 gtgtttatta gatccccgac acttaaaagt attaatttaa agaatcatat aattttagaa
     4081 aagagaggta aatgattatg aaattaaaac atattattcc taacatggag aagacatttg
     4141 gaaatttaga atatgcagga gaaggcgatg ttgaacaaaa aagaataaac ggtaaaatga
     4201 cagttatgtc tagatcatat aatctttact ctgatataca gagagcagat gatatagttg
     4261 ttgttctacc agctaatgca ggtgaaaaat cctttgaagt tgaagaaagg gtaaagttaa
     4321 taaaccctaa aattatagca gaaggatata aaataggaac tcgtggtttt acaaattata
     4381 ttttattagc agatgatata gtaaaggcct aaggaggata ggattatgag attagcaaat
     4441 ggaatagtta tagataaaga aaaaacattt ggaatgttaa agttttcagc attgagaaga
     4501 gaagtacgtg ttcaaaatga tgatgggaca ccatcaaatg agattaaaga acgtacttat
     4561 gatttaaaga gtagggggca aggaagaatg atacaagtta gtattcctgc atcaatacct
     4621 ttaaaagaat ttgattataa tgcagaagta gatatcatta atccagttgc agatacagta
     4681 gctacagcta attttaatgg tactgatgta gattggtata ttaaagctga tgatatagta
     4741 ttgaaaaata gacaggaaca attaggtgga gatttgcaaa aagatattca taagaaaaag
     4801 taggttaata taaaaagaaa ggataaaaaa tgaaatttta taaatataaa gggaatagga
     4861 taagagccag tgataaatca ctggtttttc atttttcaat aagtatattg tcaattatat
     4921 ttattacatt attgatagtt cttaatgtga aacagattgc agaaataaac tggaaaaata
     4981 taaacttgtt ttcagagaat ggattatcat tttcactcac aacttataac atattaacaa
     5041 taatattagc gattagtatt tgtctattag tcgctttttt atattatcac tatcaatatg
     5101 atagatttaa aaaattaatt catcgtcaaa aattagcaag aatgatatta gaaaataaat
     5161 ggtatgaatc tgagtctatt aaagatagtg gattttttaa agatttaaat tctaataagc
     5221 agaaagaaaa aatagtatac tttcctaaga tgtattacga attaaaggat ggattaattt
     5281 atattaaagt tgaaataact ttaggaaagt atcaggagca gttattaaat ttagaaaaga
     5341 agttagaatc tggcttatat tgtgagctaa tttctaaaga gctaaaagat tcttatattg
     5401 agtatgtttt attatatgat accattgcaa atagaatatc cattgatgaa gttaaagtta
     5461 gcaatgggag tttaaaactt atgaagtctg tatattggga gtttgataaa ttaccacata
     5521 tgttaattgc aggtggaacg ggaggaggga aaacatattt tatacttaca attatagaag
     5581 cattgttaag gacagattct atattatatg ttttagatcc taaaaatgca gatcttgctg
     5641 atttaaaaac agttatgcca aatgtttatt atagaaaaga agatatgatt tcgtgcataa
     5701 atagcttcta tgatgaaatg atgaaacgta gtgaagttat gaaaactatg gataattata
     5761 agacaggaga aaactatgct tatcttggat tgccggctaa ttttttaata tttgatgagt
     5821 atgttgcttt tatggaaatg ttaggaacta aagaaaatgc tactgtttta aataaactaa
     5881 agcaaatagt aatgcttggg agacaggcag gattttttct tatattagct tgtcaacgtc
     5941 cggatgcgaa atatcttgga gatggtatta gggatcagtt taattttagg gtagcacttg
     6001 ggaggatgtc tgaacttgga tataatatga tgtttggtga agcaaataag gaatttttct
     6061 taaaacaaat taaagggaga ggatatgtgg atgtaggtac aaatgttata agtgaatttt
     6121 ataccccatt agtacccaaa ggacatgact ttttgaaaga aatagataaa atcattaaga
     6181 gcaggcaggt agaacaatcg gcgtgcgaag cgaaagacga ttgtttgaac tagcctgcta
     6241 ttggtatggc tttgccatac caatatttat tagcccccgg aatctaacag gggggcacaa
     6301 atagactaaa aataattaaa taaatacatg gaactattga aaataaatag ttttataaaa
     6361 ttagaggtat gtgaaaaaaa ttgaaattat ttcacatacc tttttgattg gagagattta
     6421 aattgaacat agaaaaatgg gttctagatt taaaagaaaa aagaatagaa tatggagttt
     6481 ctcagaataa attagctaca tcagttggaa tcacaagaga gtatctaaat aaaattgaaa
     6541 atggaaaaat aatagttaaa aatgaaataa aagaaaaatt aatagagtct atgaaaagat
     6601 tgaatcctga ttcaccactt acaatgatga ttgattatat aagaataaga tttccaacaa
     6661 cagatgtaaa acatgttgtt aatgatatat tgaagttgaa gattgaatat atgatacatg
     6721 aagattatgg attttattct tattcagaac attatgttat aggcaatata tttgtactag
     6781 tatctcaaga tatagagaaa ggagttttaa tagagttaaa agggcaagga tgcagacaat
     6841 ttgaaaattt tttactagct cagcatagaa gttggtatga cttcctatta gatgcgttaa
     6901 tagatggagg ggttatgaag agattagacc tagcgattaa cgatatggta ggaatattag
     6961 atataaaaga actgactgat aagtgtaata atgaagaatg tatttcagta tttcgtagtt
     7021 tcaaatctta tagatcagga gaattagtaa gaaaaaatga aaagattggt atgggaaata
     7081 ccttatatat tggatcatta aaaagtgaag tttacttttg tatttatgaa aaagattatg
     7141 aacaatatat aaaactagat atacctattg atgaagcaaa aataaaaaat agatttgaaa
     7201 taagattgaa aaatgaaaga gcatattatg cggcagttga tttattagct aatagagatg
     7261 ctgaaaaaac agcattttca attataaata gatatatacg ttttgtagat aaaaataaag
     7321 ataaaaatag gagtgaatgg aagattaatg aacattggaa ctactttata ggtgaaggta
     7381 gagaaaaatt aaaattaaca acaagtccag aaccatacaa tatttataaa actttaaact
     7441 ggctatcaag acaggttgca ccaactttaa aaatgattaa aagattagat ggcttgaata
     7501 atacaacatt aattgaagat ttaataagac atgcagaatt aacagatcgt cataaaaaaa
     7561 taattcatca aaattcagta aatgttgagg atattatttg tgaataaagt aaatacaatc
     7621 tgattgtaga tagcagatga aataaaaaat tataggtgag gaggagaaga aatatgaatt
     7681 ttggacaaaa tttatataat tggtttttag cgaatgcaca gtcattagta ttaatggcta
     7741 tagttataat aggtgtatat cttggattta agagagaatt ttcaaaactt ataggatttt
     7801 tagtagttgc attaattgca gtaggacttg tatttaatgc agcaggagtt aaagacatat
     7861 tacttaagtt gtttaataaa attataggag cttagaaata ttaatcaata tgatataata
     7921 tttttataaa ttttaagatt agataatatt ttataaacta gtatgtagta agtaaaattt
     7981 cagtaaataa gtaaattggg gaggaataat atggaatgga agagatataa aaattttata
     8041 tcattaatag tgacaattct aatagttatt tggcttgtgc ttaaatttat ttttaaaaat
     8101 caatttagtg attttataag tgctataatt atgactataa taggtattac ttattttagt
     8161 acttatataa agtcctatag aaaaaataaa aatagatttg aaattttatt tatattactt
     8221 agttttatta tgagtatatc gggtgtctta ttggttattg gatttttcgt aaattaagct
     8281 taaataagaa tgccttttct attaaaaagc agtagatttt tatctactgc tttttatatt
     8341 gaaatttaat aaaaggatgt gagatagtag tatggaagaa atgagagtat atattgcaaa
     8401 cttaggaaaa tacaatgaag gtgaattagt tggagagtgg tttacaccac cgattgattg
     8461 ggatgatttg aaggaacgta taggattaaa tagtaaatat gaagaatatg caatacatga
     8521 ttgggagtta ccttttgaaa tcagtgagta tacttcaata gaagagatta atcgtttatg
     8581 ttctatggct gaagaattag catctacaga tattggtgaa gctattagtg atattcaaaa
     8641 agcttttttc tctagctttg aagaattata tgaacataag gatgatatta tttattatcc
     8701 aggatgtagt gacatgtctg atgttgcata ttatcttata gatgattgta atatgttggg
     8761 agaaatacct gaaaacgtga gaaactatat taactatgaa gcatatgctc gtgatttaga
     8821 gattaattat agttttgtag ttactaatca tggagtattt gagtatgcta attaaagttt
     8881 aatagttttt atagaagaca gttagaatta tactaactgt cttttaattt taaaattagt
     8941 aaggaggggt gtatttgaaa aaaattaagt catatacagg tatttggaat gtagagaaag
     9001 ttatatatgc aataaatgat tttcaacttc catttccaat tacttttaca caaattacat
     9061 ggtttatatt aacactattt ataatcatat tatttggtaa gttacctcca ttatctatga
     9121 tagaaggagt atttttgaag tatcttggaa taccagtttt tataacttgg tttatgtctc
     9181 aaaagacttt tgatggaaag aaaccatatg gatttttaaa gtcaataatc ttatacgcat
     9241 ttagacctaa aataacatat gcaggaaaaa gaattaaatt taaaaatgaa actattgatg
     9301 aaaacataac tatagtaagg agtgaattat atgttcccga taaaatatat agagaacaat
     9361 cttgtattta ataaggatgg agaatgtttt gcatattatg aattaatacc atataactat
     9421 tcattcttat cagcagaaca aaaatttatt gtacatgata gttttaggca attaatagca
     9481 caaaatagaa atggaaaaat tcatgcactt caaatagcaa ctgaaagttc tataagagct
     9541 acacaagaaa ggtcaaagaa aaatattaca gggaaattaa aagaaattgc ttacgaaaaa
     9601 atagatgagc aaacagaagc acttgtttct atgattggcg ataatcaaat tgattatcgc
     9661 ttttttattg gatttaagct tttagttaca gaagaagaca tgaatataaa gaatataaaa
     9721 aagtcaatat tagctaatct atcagatttt atatatgatg ttaataataa attgatggga
     9781 gactttgtca gtataaataa tgatgagatt atgagatttt caaaaatgga aaagttatta
     9841 gaaaataaaa tttcaagaag attcaaagtt agaaggttag ataaaaatga ttttggatat
     9901 ttaattgaac ataattatgg aaagaaaggt acagcttttg aagactatca atatcactta
     9961 cctataaaga aacttaaaag agaaacatta gtaaagaaat atgaccttat aaagccaact
    10021 aggtgtctta tagaagagaa gcaaagatac ctaaaaatta cacatgagga ttcagaaagt
    10081 tttgtagctt attttactat tgaaagtatt agtggcgaaa tggactttcc atctagtgag
    10141 atattttatt atcagcaaca gcagtttaca tttcctattg atactagtat gaatgttgaa
    10201 attgtaacaa ataaaaaggc tttaactaca gttagaaata agaaaaaaga attaagagat
    10261 ttagataatc atgcttggca ggcaggaaat gaaacaggaa caaatgtggt agaagcatta
    10321 gatgctgtaa atgagcttga aaataattta gatcaaacaa aagaatcaat gtataagtta
    10381 agttatgtaa ttagggtatc agcagatagc attgatgaat taaaacgccg ttgtgatgaa
    10441 gttaaagatt tttatgatga tttaaatgta aagttagtac gtccaatggg agatatgata
    10501 ggatttcata atgaatttat accttcaagt aaaagatata taaatgatta tattcaatat
    10561 gtaactagtg attttctagc aggattagga tttggggcaa ctcaacaact tggagaaaat
    10621 gaagggatat atattggata tagtttagat acaggaagaa atgtatatct gaaaccatat
    10681 cttgcaagcc aaggagtgaa aggttctgta actaatgcat tggcagcagc attcattgga
    10741 tctttaggag gggggaaatc atttgctaat aacttacttg tttactattc agtattgttt
    10801 ggaggacaag ctgttttggt agatccaaaa gctgaacgtg gtaaatggaa agaaaattta
    10861 ccagaaatag ctaatgaaat aaatattgtt aatttaacaa gtgatataga aaataaagga
    10921 atgttagatc catatgtaat tatgaaagaa gtaaaagata gtgaaagttt agctattgat
    10981 atactaactt ttcttacagg aataagtagt agagatggag agaagtttcc tatacttaga
    11041 aaagcaataa gaaatgttac tctgagtaaa tgtagaggac ttttaaaagt aatagatgaa
    11101 ttaaggaaag ataattctca aatatcaaat aacattgcag atcatattga aagttttaca
    11161 gactatgatt ttgcacaatt acttttctca gatggaaata ttaaaggttc aattaatcta
    11221 gaaaaacaaa tgaatataat acaagttaca gatcttgttt tgccagataa agatacttct
    11281 tttgaagaat atacaacaat ggaaagtctt tcagtagcta tgttgatagt aattagtact
    11341 tttgcattag attttattca ttcagataga agtattttta aaatagtaga tttagatgaa
    11401 gcttggacat ttcttaatgt agcacaagga aaaactttat ctaataaatt agttagagca
    11461 ggacgtgcta tgaatgcagg agtgtatttt gtaactcaaa atgcaggtga tgtatcagat
    11521 gaaaatttaa agaataatat tggattaaaa tttgcattta gaagtacaga tattaatgaa
    11581 attaagagaa cacttgaatt tttcggagtt gataaagaag atgataacaa tcaaaaaaga
    11641 ttaagagagt tagaaaatgg gcagtgttta atacaagatt tatatggacg agttggagta
    11701 atacaaatac atccagtttt tcaagagctt cttactgcat ttgatacaag accacctata
    11761 agaaaagagg tggattaatg aaatctaagt catttagaaa tttaagttgg ataactgttt
    11821 taatagtaat acttgtatta ttttttatct atataagcga atctgtagtt tatgcagaag
    11881 gactagtaga tgaaactata aattcaaata atatatattc aatgtatcct ttaagaaatt
    11941 atcagcttga tttttatgtt gataattctt gggtatggct tccttggaat tggactgatg
    12001 gaataggcaa atcagtgcag tatggattat attgcataac aaattttatt tggataatca
    12061 gcttatatct atcaaatgca actggatatg ttgttcaaga agcatataaa ttagatttta
    12121 ttaatgatat ggcagattcg ataagtaaga gtatgcaaac aattgctgga atcaatcaaa
    12181 atggaatatc taatagtggt ttttatatag gatttctact tctatttgtt ttaattctag
    12241 gaatttacgt agcatataca ggattgatta aaaaagaaac tagtaaggca attaattctg
    12301 ttattaattt tgttgtgata tttataatat ctgcatcgtt tattgcttat gctcctgatt
    12361 atatcaaaaa agtaaatgat ttttcttctg atattagcac agctgttttg aatttaggaa
    12421 cgaaaattgt aacttctaat tcaaataatg aaggcaaaga tagtataaag ttaattagag
    12481 ataatttatt ttctatacaa gtacagcaac catggatatt attacaatat ggaaattcta
    12541 atatagaaga aatcggaaaa gaaagagtag agaagttact gtctacagat ccaagtgaag
    12601 atgatggaaa aaaacgagag gaaatagtta aaactgaaat agaagaaaaa cataataata
    12661 atttgactat aaccgaagtt atgagtagat taggtaaggt attttttcta tttatattta
    12721 atataggaat aaccattttt gtatttttat tagtatctat gatgatattt tcacagatac
    12781 tttttattat tttggctata tttttaccat taagtttttt actatctatg ataccctcat
    12841 atgaaagtat tggaagacag gcaattgtaa agttattcaa tactataatg atgagagctg
    12901 gaataactct tattataact atagcgttta gtatatcaac aatgttttat acaatatcca
    12961 cagattatcc cttctttatg attgcatttt tacaggtagt cacatttgca ggaatttata
    13021 tgaagttagg agatattatg ggaatgttta gcttacactc aggagatagt cagaatatga
    13081 gtagtcgtat gtttagaaga ccatatagaa caatgcgaaa taagagtaaa aatatagaac
    13141 gcaatataag gaaggcttta atatctagag gaaaagataa agaaagatta tcatctaaca
    13201 taaataatcc acaaaggaat gtaaataata cacaaaaaag aactggttat ccacacagta
    13261 gagagaatta tgataaatca gttagtaaag aaagtttttc aaaacgtatt ggaaatactg
    13321 ttggagcatt tatggataca aagaaaaata tatccgataa atcaaaacaa gttaaagaaa
    13381 atatacaaga tatacctgtg caagctaaat atgcagttca taatacaaaa gaaaaagcta
    13441 aagataatat atctgatttc aaaagaggta tcgtagaaga aaaagaaaaa cgtcaacaag
    13501 atagaaataa aaagcagcaa aatcatagag agactatagc tcaaaaaaga gctaagttag
    13561 gaaatatagt agaagctaaa gaaaaaaatt ctacagataa caaaaatata gatggctact
    13621 cagattcaaa atataaacct tcaattaatt ctgaaagagt gaaaaagaaa aaaggagaaa
    13681 atattagacc aacagtttca aaaaaaatat atgatgagaa agacagagaa aaaagtaatt
    13741 ctataaataa aataaaaaga tctacagaga aacctatcca taaagaaaga gatattgtaa
    13801 atcaaagcga agaactatct aataaagttg aaaataatca aagtgtaaaa gtaaaaaaca
    13861 gtaaaactat gaagatagaa cgacataata ttaattctaa tttaaaagga cctaagtata
    13921 atcacataga tattaaagag agaaaaataa aaaataaatc taattttact cctgagaata
    13981 aggtgagaaa acaatgaaaa taaagctgat aactattttt attagtatat tcttatttat
    14041 atttggagta attttaatgt ttgtaccttt aatatcagat gaggatcata acggaggtat
    14101 gaatcaaaat ataattggaa tgaatttatc accagaagtt ctaaaattta agcctatggt
    14161 tgaaagatat gctaagaaat atgatgttca agagtatata aattacttac ttgctattat
    14221 gcaagttgaa tcaggtggaa ctttagttga tgttatgcaa tcttcagaat cactagggct
    14281 tccaccaaat actttaggaa cggaagaatc aatagaacaa ggatgtaggt atttttcaaa
    14341 tcttttaaag acatcaaaag aaaaaggatg tgatactaat tcaactatac aagcatataa
    14401 ctatggattt ggatatctaa actatattaa aagtaatgga aaaattcata cttatagttt
    14461 agcagaaaac tttgcaaaaa atatggcaca gggagttagg gtaacttata ctaatccaat
    14521 agctgttgct aaaaatggtg ggtggagata taaatatgga aatcaatttt acgtagagtt
    14581 agtacttcaa tatgttggaa caagcgaatt taatgatgaa acagttaaaa taatcataaa
    14641 tgaagcattg aagtataaag gttggaaata tgtatttgga ggagataatc caaatacttc
    14701 ttttgattgt agcggattaa ctcaatggtg ttttaaaaaa gcaggaataa atttaccaag
    14761 aacagcacaa caacaatatg atattatgca acatattgca ctagatcaag caaaaccagg
    14821 agatttagtg ttttttcatg gtacttataa tgctggaact tatgtaactc atgttggaat
    14881 atatgttgga aataacaaga tgtatcatgc aggagatcct attgtatatg aagatttaac
    14941 agatagctat tggcaaaaac atttaattgg agcaggaaga ataaaaaaat agatggggtg
    15001 aaaatatgtt aagaaaaaag gataaaaaac ataaggaaaa gaagatacta gttataaggg
    15061 gaggtacaaa taaaaagttt gtaattgctt tatggattat tttaattgta agtataacat
    15121 ttagtgttta taaaaacttt actgcaatag ataaacatac tatccatgaa aagaagatta
    15181 ttgaacagaa aattatagat acaaatggaa ttgaaaattt tgtgaaaaac tttgctaaag
    15241 aatattattc atgggagaat aacaaagaat ctattgaaag aagaacagct aaaattaatg
    15301 agtatcttac aaaagaattg caggaactaa atatagatac tgtaagaagt gatattccaa
    15361 caaactctag tgtaaaagac gttcagatat ggagtgtaaa tcagattaaa gaaaatgaat
    15421 ttgaagttaa atattctgtg gagcaaacta taaaagaagg agaggaaata aaagatatta
    15481 cttcttcgta tgaagttgta gttaaaatag acaacactgg aaatatgata attataaaaa
    15541 atcctacatt atcaaaaatg tcagaaaaat taaattatga atctaaaaaa gaagaaacag
    15601 atagcacaat agagcaaaaa acaattaaag aaattacaga atttttaaat acattcttta
    15661 agctatatcc tactgcatct aaaaatgagt tagaatacta tgtaaaagga gatgcactta
    15721 aacctataaa tggagattat atcttttcag aattagtaaa tcctattttc aaaaaagaat
    15781 ggaaaaatat aaaggtatta acaggtgtaa aatatataga tcaacaaaca aaaatgattc
    15841 aagtttctca atatgagctt gttttagaga aagataataa ttggattatt gttaaataaa
    15901 aataaatggt atgtacaaaa atttatatgt acataccatt tattttcttt gatttaaatg
    15961 ttagtaatga catttaaaag tagatttaag cttagagata gcagtttttt tgtataataa
    16021 aaatgattta catatatcta tatttttatc ataataaaaa aagaaaaaat tattaccatt
    16081 aaattatata aaatagtatt taaaaaaata tgataaaata aatattatac aaaatgttgg
    16141 aggaaataaa atttatgatt acaggtgagt taaaaagtaa ggtagataag atttgggaaa
    16201 ccttttggac tgggggactt acaaatccat tagaagttat agagcagttt acatatcttt
    16261 tatttattaa aggactagat gatgtagaaa caaaaaatga agcagaagct gttttattag
    16321 gatttgaacc agaaagaata ttttcagata atgagcaaga attaagatgg agcaaattct
    16381 ctaatgaggg agatgctcaa aagatgtatg atatagttca aaataaggtt ttccctttta
    16441 ttaagaattt acatggaaat aaggaatcag catatgcaaa gtatatgggg gatgctatat
    16501 tcaaaatacc aactccactt atgctttcaa aaatagttga tggaataaat ggaatagagt
    16561 ttaaaaagga gaatgatact aaaggagatt tatatgaata tttactatca aagttatctt
    16621 cagctggtac aaatggtcag tttagaacac caagacacat tattgatatg atagtaaagt
    16681 taatgaagcc aacaccagag gatataatag ttgacccagc agcaggttca gcaggtttct
    16741 tagtatcttc tcaacaatat ttaagagata atcataatga tttattttat gttcaaggat
    16801 taaaggaaca ttttaataat actatgttct atggatttga tatggacaga actatgctta
    16861 gaattggagc tatgaatatg atgcttcatg gagttgataa tcctaacata gaatataagg
    16921 attccttatc agaggttaat acagataaag aaaaatttac tttagtatta gcaaacccac
    16981 catttaaagg aagcttagac tatgaagcag taagtgcaga ccttttaaaa gtaacaaaaa
    17041 ctaagaaaac agaattatta ttcctagcat tattccttag aatattaaaa acagggggaa
    17101 gatgtgcatc aatagttcca gatggagttt tatttggaag cactaaagga cacaaggata
    17161 taagaaaaga aatagtagaa aatcataaac tagaagcaat aatatcaatg ccaagtggtg
    17221 tgttcaagcc atatgcaggg gtaagtacag ccattatgat attcacaaaa acaggctcag
    17281 gtggaacaga taaggtttgg ttctatgata tgaaagctga tggattctca ttagatgata
    17341 aaagaaatcc agtagaagaa aatgacataa atgatataat agaaagattc tcaaacttag
    17401 aaaatgaaga agatagaaaa agaacagagc aaagcttttt tgtaccagta gaagaaataa
    17461 gagaaaatgg atatgacctt tcaatcaata agtataaaga aatagaatat gaagaagtag
    17521 tttatgatgc tccaagtgtt attttaggaa gaatcaagga acttgaaaaa gaaataacta
    17581 aaggtttaga agaattagaa ggaatgcttg aggtgtaaaa tgaagtatat taagttaaaa
    17641 gatatatgtg atttacaaaa tggatttgct tttaaatcaa gtgattatat tgatagctca
    17701 acagtattaa attgtagaat gagtaatata agaccaaatg caaaatttga tataatgtat
    17761 aatcctaagt atttgccaga aaattttgtt gaaagatata aagaatatat tttaaatgat
    17821 ggtgacctca taattgctat gacagatttg gcaaatgatc ctaaaatttt aggggtgcca
    17881 actattgttg agactaaagg gaatacttta ttgttaaatc aacgtgtagg aaaattaata
    17941 attttagatg ataaattagt tgataaatac tatttaaaat attcattaag cttacctcag
    18001 cataagcctt attataagaa atttgctggt ggaggtttac aaataaatat cggtaaagaa
    18061 caaatattaa atttgaaaat tccaatttca aatatagaaa ttcaaagaaa agtagttgaa
    18121 atattagata aatcacaaga attaatagac aaaagaaaaa aacaaataga agaattagat
    18181 ttattaatta aatcgaaatt tatagagatg tttggtgacc caataaataa tcccatgaag
    18241 tgggaggtta aaaaaatgaa agatatttca gttaatataa tgagtggaaa cacaccaaaa
    18301 ggtggagaac aagtttatgt tgaaagtgga attatgtttt taagaagtca aaatgtatgg
    18361 aaaaacaaat tattattaga tgatgtagct tatatagacc aagcaacaca taataaaatg
    18421 aaaaaaagta gtttaaaaaa taaagatata ctaatgacta aaacaggtag aataaataca
    18481 gaaaatagta gtttaggtag agcagcaatg tttttaggag aagatgatag tgccaatatt
    18541 aatggacacg tatatttaat aaggcttcaa gaacaaataa ttaatgagtt tgttttgtat
    18601 attcttacaa gtaaagaata tagaggatat atcagaagtg tctgtgttgg aggaattgat
    18661 aaaagacagt taaataaaga acatatagaa gattttccta ttatttttcc accgatagaa
    18721 aagcaaaaga agtttgttag ctatgttaat caagtagaca aattgaaatt taaaatggag
    18781 aatagtttaa aagaattaga agataatttt aattctttga tgcaaaaagc atttaaggga
    18841 gagctattta attaatataa gattgaagga gaaggtatta gtgaaggaat ttgagttgaa
    18901 tttatataaa gatatggttg atatctataa aaaggcataa gaaagttatg gatataaggc
    18961 tcgtagattc ttgcaaatga taaatgataa aggagcttta ataacatcta aaactcttat
    19021 aaataaagat tctaatacag agggatttat agctttatgg gaaaataata gattggattt
    19081 atctttagag gcgcttatag taagtggaaa gtatgatgaa ctttttactg aagaagaaaa
    19141 aaacatttgc aaatatagat taagagatta tggatataag atttaagtta ggggaataaa
    19201 ggttatatgg ataataatat gagaaatgaa atagaatcat ggggaattcc taatatagat
    19261 gagctaaaag gcagaaatat aaagtttgtc tttaattcaa gtgaaattaa taaggaaaaa
    19321 ggctcttttg tagaattaca ttgtgaaaag gagtctgtaa aattttgttt atatgatttt
    19381 actaatgaca tagaaatatt tactatggag ttttttgcaa gacctaaatc tttattaatg
    19441 ctaactaatg agttaccaag cgttaagcta gaattattaa atgttaatga ttttaactta
    19501 agaagaaaag ggatagcatc ttattactta gataggttta aagaatatgc tataaaacat
    19561 aagtattaat gtataagagt tacgcctaat cccaatgcac ctgtatttca aggagaaagc
    19621 aaagacaatt cacttgagat agatgagtta gtgaattttt ataaaagtaa atcaacacct
    19681 aaaatgccga taaaagtttt agaattataa atgtttagat agttaagtta aaagcttagc
    19741 tatctttttt atttgattta ataatgtata aattgatata attttactat aattaggaat
    19801 taatacattt taatataaaa atctgaggag aagtttaatg gggagaaatt ttgaattttt
    19861 aaaaggtaag aagcagtttg atggatttag ggcacaatgc ttcgaagctg aaaagagtat
    19921 tattgtaagt ccagctactt gtgcaatttt atcaagaaga gctttagaac ttgctgttaa
    19981 atgggtttat ggtaatgatt cagagttagt tttaccatac caagatagta taagttcatt
    20041 aattcataat gaaagttttc gtgagcttat tgattcaagt atgtttaata ttttaaagta
    20101 cataataaag cttggaaatg tagctgttca tactaataaa cctattagta gagaggaagc
    20161 tatactatca ttacacaatt tacatcaatt tgttagttgg atagattatt gctatgcaga
    20221 taattatact gctactaatt ttgatgaaag tttacttcat gaaggtgaca ataaaagaac
    20281 tagaccagag gaacttaata atctttatga aaaattaagc tctaaggata gaaagcttga
    20341 agaagtaatt gatgaaaata aagagaaaag aaaacttgga actgagaaga gaaaagagaa
    20401 tacagcaaat aatactttta aagtggataa gataagtgaa gctgaaacta gaaaaaggta
    20461 tattgatgta gagttaaaat tatctgggtg gacttttggt aaggatattc aaatagaagt
    20521 agaagtaaat ggaatgccaa atgaatcagg attaggatat gttgactatg ttctttatgg
    20581 agataatgga aaacctttag ctgtggttga agctaagagg acaagcgttg atgaaaagaa
    20641 ggggcaacaa caagctaaac tttatgctga ttgtttagaa aaacagtata atcaaagacc
    20701 agtaatattc ttaactaatg gatttagata ttatatatgg gatgactgtg gagatagaga
    20761 agtatttgga ttctataaaa aatcagaact tcaattgatg atagatagaa gaacatctaa
    20821 gaaaagttta aaagatataa agattagtga tgaaatatca aatagatatt atcaaaagga
    20881 agcaattact tcagtagcag aagcttttga aagaaatcaa agaacagctc tattagtttg
    20941 tgcaactgga acaggtaaaa caaggactgc tatttctata gttgatgttt tatcaagaca
    21001 taactgggta aaaaatgttt tattcttagc agatagaaaa gcgctagtta aacaagctaa
    21061 gaaaaacttc tctaaactat tacctaatct atcactatgt gatttattag atagtagaga
    21121 taatccagaa gaatcaagaa tggttttttc aacttatcct acaatgatga atgccataga
    21181 cgatactaaa tctaaagatg gagatagact atttactcca gggcattttg atttaataat
    21241 aattgatgaa tcacatagaa gtatttataa gaaatataaa tcagtatttg attattttga
    21301 tgcttattta ataggattaa ctgctactcc aagagatgag atagataaaa atacttatga
    21361 tatattcaat cttgaaaaag gagtaccgac ttatgcatat gagtatgaaa aggctgtaga
    21421 agataggtat ttagttgatt atagaactat tgaaattaaa tctaaaattc ttgaaggtgg
    21481 aattaagtat gatgacttaa gccaagaaga aaaagaagag tatgaaatga tgtttgatga
    21541 tgatgaaaat attggagatg aaataagtag ttctgctata aatcaatggt tattcaatac
    21601 tgatacaata gacttagttt taaataaact tatgactgat ggcttgaaaa ttgaaggtgg
    21661 agaaaagctt ggtaaaacaa taatttttgc taagaattct agacatgcta aatctatagt
    21721 tgaaagattt aataagcttt atccagagta tgggggaaca tttgcaagag ttgtagaaca
    21781 taaaactcct tatgttgaaa atataataga tgatttttca gataaatata aaatgccaca
    21841 aatagctgtt tcagtagata tgcttgatac aggaatagat ataccagaga tattaaatct
    21901 tgtattcttc aaaaaagtaa gaagtaaaac taagttctgg cagatgatag gtaggggaac
    21961 tagattgtgt cctgacttac ttggaatagg ttttgataaa gaagagttct taatatttga
    22021 tttctgtaat aactttgaat tctttagagt taatcctaaa ggttttgaag gtaataaagt
    22081 agaaagtctt acagaaagat tatttaatgt aaaagttgat atagttaaag aacttcaaga
    22141 tataaaatat caagatgatg aaaagtatgt taattataga aatgaattgt taggagaatt
    22201 aataaaatct gtacaagcgt taaatgatga aagttattta gttagaatga acataggtgt
    22261 tgttcataag tataaagatg agaaggcttg gaatgctatt ggtgccatag agcaaaatga
    22321 gattaaaaaa catatatctc caataattaa atctataaat gatgatgaac tagctaaaag
    22381 atttgattta cttatatatg tgattcagtt agctaactta caaaatattg ttttcaagaa
    22441 gggtataaag tcagtaatgg aaacagctga taagttatct aagttaggaa caataccaga
    22501 gataaaagaa aagaaagcta ttatagaaag aatagtagag cctgaattct gggaatgtgc
    22561 cgatatattt gatatggaga atgtgagaga ggctttaaga gatttaataa agtatttaga
    22621 aaaaagcact caaggaatat attacacaga atttagtgat tcaataatat cagaagaaag
    22681 ccactcatca atgtacaatg ttaatgacct taaaagttat agaaagaaag ttgaatttta
    22741 tctaaaagaa cataaagatg agttagctat atacaaactt agaaacaata aaaaattaac
    22801 tgagcaagat atacaaacat tagaaaaagt tctatttaat gaacttggaa gcaaagaaga
    22861 atatgaaaaa gaatatggaa aaacaccagt aactcaatta gtaagaaaaa tactaggatt
    22921 agatagaaat gctgctaatg aagctttctc agaattctta aataataaaa gtttcaatag
    22981 ttcacaaata cactttgtta agcttattgt tgattatatt gttaagaatg gatttataga
    23041 agataataag attttaacag aagatccatt cagaagtgtt gggagtataa tagatttatt
    23101 cccgaataat gttagtgaaa gagcaaaact tataaaaact ataaacgaaa taagagaaaa
    23161 tacattatag ttttatgaat taaatagaaa aattgtattt ttaatttaaa aggtgctgtt
    23221 ttaattaagt gttgactagt gatatatttg tgaaaattga ttataatata agtataacga
    23281 ctttaggtgg agtatctatg agcaatgttt cagaattatt agcacaggca aaataattac
    23341 tgaagcagga gcaagaggaa ctattatcct agttagagga atttgtaatc ttaagctcta
    23401 aagctaatca gataactaaa gaagtaaaag aaatgaggtt ttctaaaggt agagtttgtt
    23461 gtcactgaaa tgggaaaaat aatggtgttc aaagatatct atgtagagat tacaacaaaa
    23521 gcttttcaga tcttacaagt ttagcaacgt atagaagaaa aaagggatta gataaatgac
    23581 ttaagtattc aatatgtaca cttaatggct accttataag aaaatgtgct gaaacagtgg
    23641 gaattaatat tgttacaagt ttcttttgga ggcacaagct taaattggat tagtgatttt
    23701 attggagtag gcttagttga tggtgttgta gaagctgatt aagtattctt cttctactca
    23761 tataaagcta ctaagtcatc aaatattaca agaccatctc gtaaaatagg taataggtta
    23821 agaagaggtg gactagtaat gaacaagtgt gtattgcaac tgattttgat aggcaagata
    23881 attttattat agaattactt tgtgtaggta gaatgacttc tgagtagttg aaaaaacttt
    23941 acgataaccg tatagaaaat gaaactattc tatgcacaga ttatcacaaa agttatattc
    24001 aattttcgac agatattaga ttggagtata agcaaattag aataggaaga tataaagagg
    24061 atatatacca tattcaacat ataaatagcc tacactccaa tttaaaaggt tggatgagaa
    24121 ggtttaattg ggttgcaaca aaatacttaa gcaactatat gaaatagcat aaatggatgg
    24181 gcacttttag atataagaaa gagataatta taactaaaaa attgttagtt aatagcatat
    24241 ctcccataaa tatacgagag taaatgaatt atgattaact attaatacaa taaggaattt
    24301 tttaaatagt ttcttttaaa atggatttat ttactctatt aatatatatt gtataattta
    24361 tttataagct ataacttaat ttataagggg gaactataag attaacttat gaagatttaa
    24421 aaaaatgaaa tagtataaag tttagaaaac aatagaaatt aggttctatc cacctcatca
    24481 aataatattg tatcatcaag gtctatgagt ataataaata taggattgta tatattttct
    24541 ctataagaca aaatctatac atatctgaaa tgatagctga acaaattgga tttatgtaat
    24601 acaagaaaaa agctttgcac tgattgaatt aaaatattta tttacatcac aaatttaata
    24661 aaaatgtgag aaaagagtgg agaaggtgca ttgtccactc tttttgagtt caatatcaac
    24721 atttatttaa aacagagcca tttaaaaaaa taataaataa tga
//
LOCUS       DAKBNN010000039        24472 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000039 DAKBNN010000000
VERSION     DAKBNN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.039"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            21..404
                     /locus_tag="OUB02_001363"
     CDS             21..404
                     /locus_tag="OUB02_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT8885130.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            406..756
                     /locus_tag="OUB02_001364"
     CDS             406..756
                     /locus_tag="OUB02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885131.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(857..1315)
                     /locus_tag="OUB02_001365"
     CDS             complement(857..1315)
                     /locus_tag="OUB02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885132.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEERFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1360..2148)
                     /locus_tag="OUB02_001366"
     CDS             complement(1360..2148)
                     /locus_tag="OUB02_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8885133.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTTVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2287..2724)
                     /locus_tag="OUB02_001367"
     CDS             complement(2287..2724)
                     /locus_tag="OUB02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HCT8885134.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3546..4055
                     /locus_tag="OUB02_001368"
     CDS             3546..4055
                     /locus_tag="OUB02_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885135.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4453..4659
                     /locus_tag="OUB02_001369"
     CDS             4453..4659
                     /locus_tag="OUB02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HCT8885136.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTKLSLCYLTNGAQK"
     gene            complement(4953..5753)
                     /locus_tag="OUB02_001370"
     CDS             complement(4953..5753)
                     /locus_tag="OUB02_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885137.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(5948..7546)
                     /locus_tag="OUB02_001371"
     CDS             complement(5948..7546)
                     /locus_tag="OUB02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT8885138.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            7874..8050
                     /locus_tag="OUB02_001372"
     CDS             7874..8050
                     /locus_tag="OUB02_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885139.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8224..9354
                     /locus_tag="OUB02_001373"
     CDS             8224..9354
                     /locus_tag="OUB02_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCT8885140.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            9595..9945
                     /locus_tag="OUB02_001374"
     CDS             9595..9945
                     /locus_tag="OUB02_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCT8885141.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            9953..10402
                     /locus_tag="OUB02_001375"
     CDS             9953..10402
                     /locus_tag="OUB02_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCT8885142.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            10504..11079
                     /locus_tag="OUB02_001376"
     CDS             10504..11079
                     /locus_tag="OUB02_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCT8885143.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11072..12481
                     /locus_tag="OUB02_001377"
     CDS             11072..12481
                     /locus_tag="OUB02_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HCT8885144.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            12665..14098
                     /gene="eutA"
                     /locus_tag="OUB02_001378"
     CDS             12665..14098
                     /gene="eutA"
                     /locus_tag="OUB02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCT8885145.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPVVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14116..15480
                     /locus_tag="OUB02_001379"
     CDS             14116..15480
                     /locus_tag="OUB02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCT8885146.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            15493..16374
                     /gene="eutC"
                     /locus_tag="OUB02_001380"
     CDS             15493..16374
                     /gene="eutC"
                     /locus_tag="OUB02_001380"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCT8885147.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            16394..17047
                     /gene="eutL"
                     /locus_tag="OUB02_001381"
     CDS             16394..17047
                     /gene="eutL"
                     /locus_tag="OUB02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCT8885148.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17058..17759
                     /locus_tag="OUB02_001382"
     CDS             17058..17759
                     /locus_tag="OUB02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCT8885149.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            17762..19231
                     /locus_tag="OUB02_001383"
     CDS             17762..19231
                     /locus_tag="OUB02_001383"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HCT8885150.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYKTLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19327..19614
                     /gene="eutM"
                     /locus_tag="OUB02_001384"
     CDS             19327..19614
                     /gene="eutM"
                     /locus_tag="OUB02_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCT8885151.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            19741..20502
                     /locus_tag="OUB02_001385"
     CDS             19741..20502
                     /locus_tag="OUB02_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HCT8885152.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            20515..21144
                     /locus_tag="OUB02_001386"
     CDS             20515..21144
                     /locus_tag="OUB02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCT8885153.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21186..21911
                     /locus_tag="OUB02_001387"
     CDS             21186..21911
                     /locus_tag="OUB02_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HCT8885154.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            21924..22196
                     /locus_tag="OUB02_001388"
     CDS             21924..22196
                     /locus_tag="OUB02_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HCT8885155.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22189..22740
                     /locus_tag="OUB02_001389"
     CDS             22189..22740
                     /locus_tag="OUB02_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCT8885156.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            22752..23843
                     /gene="eutH"
                     /locus_tag="OUB02_001390"
     CDS             22752..23843
                     /gene="eutH"
                     /locus_tag="OUB02_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCT8885157.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            23845..24318
                     /locus_tag="OUB02_001391"
     CDS             23845..24318
                     /locus_tag="OUB02_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCT8885158.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT         9629 a         3103 c         4074 g         7666 t
ORIGIN      
        1 ataataagga gcgattaaac atgaatctat cgaagaaaat agaagataaa agaaaagcta
       61 aacagagagc tgaaaaagtt aagaaagcta aaattgctac agctggtgtg gtattaggag
      121 ctgttactgg tgcagtgagt ggagtacttt tggctcctaa atctggaaaa gaaacaagag
      181 aggatataaa agatgcttca caacaaattg ctgaaaagat aaatatgaaa actgtagacg
      241 taaaaggcaa agtatctgag aagctagagg ataaaaaagg aaactttata gaatcaaaga
      301 aaaaaataaa aaaatatata gataacaaaa agagtgttga aaattctagt gaggaagata
      361 aaataataga tatagttgaa ccagttagtg ttactgagga ataatatgga caatacaata
      421 acgattaaca taagtataat atatgtatta ttagctatac ttgcttgtat tgctttagtt
      481 tatttaatta ttgtacttgt taaactacat aagattttaa ataatgttgg aactatgtta
      541 aatgaaaata ctcaaaatat aaatacaact ttaaataatc ttcctaatgt tgtagaaact
      601 tttggagaag taggagaaaa tatgaaagat gtaacagaag tagttactga agttacagca
      661 gattttcttg ttactaaaga cagtgtcaaa tcaaatatgg atgttatagt tgatatattg
      721 actataatta aaaacatttt tctagataaa aaataataag ttaatataaa ataagcctat
      781 aagatataat ttaaacgacc ttaaaatagc atttataagg tcgtttttta ttagcttgat
      841 ttgacactat gtgtttttaa taattggtaa tattcctctt tatcatttga acatatatat
      901 ctatgcttgt tgtttcaatt acatccataa gaacatcttt acattcaagc tcaaaatctt
      961 ccattccttc taaatattca tcatcttcat caaaaaagat ataagaatta tatagccatt
     1021 cttcaaatct ttcttcaagt atatcccaat acgaactcca aaataatcca caacctgata
     1081 tataagatgc tctactttga tttatataaa cctcatttag aagcttttgt aatctactgc
     1141 tataaagaaa atctgctctt ccactgcttg atattaattc atttaaatct tctattgaca
     1201 ttaattcaac tttaccactt ttatcataag gagtataatt ttcatcaacc catttatgtg
     1261 gtttgttttc tctgcatact gttgctatca actctacaac ttttgttctt atcatttttt
     1321 tatcctaact tgtttatttt ttataaaata atcatgattt tataatttat ctatttcttg
     1381 taatgctaat ttaacagttt catatctatc agcaccattt attactacat caccttttac
     1441 aagtttatta gcttctccac ctacaacata aaccttttta cctaaacctt gcttataact
     1501 agacgcctca attattaaag aatctttcaa ttgccaatgt aaaatctgag cagccacttt
     1561 atcagcatca cctgtataaa cgattacatt ttcatacact ttattttccc ctcccccttg
     1621 agaatttgat atagatttgt ttagtatacc ttctgctatc aatttagcaa ctttatcttt
     1681 attattaaca taataatctg tatctgcttt actatctaca aaacatactt ctattaatat
     1741 tgctggtgca atagtatgat taagccaata aagacctcta acatcagact ttgcacccct
     1801 atctttgaat actgtagcta acctagtatt cactctttct gcataggttt tgccattatt
     1861 agtcttatat attgtttctg tccctactgt agttgaagtt gtagcatttg aattaaaatg
     1921 aatctgaaca gccaaatcag tttcctgttt atttgctata gcacattggt ctgctaaatg
     1981 attacttgat tcatcaactc ttcctgtata aacagtagct ccacctattt taagccattt
     2041 aacaattaag tcattcagta ttcttgtttc tttgctttcg tttatatacc caacagctcc
     2101 tgtacctttt ccaattaaag tgtgccctgg tattattact actttcaaac taacatctcc
     2161 tttacaatta aatttaactt ctatttaata tatgcataac ctagaatttt gtttcttttt
     2221 atatcttata cttttattat aacccatttt gtaaaaatat agaatattaa gtaaataatg
     2281 ttaaaattat aattttgtat ataagacatt acttgattgc aataatttta tagcatattc
     2341 atctattcta tatccagttc tataatatat atgtttaatt ccagcttgaa ttatagcctt
     2401 agtacaattt aagcaaggaa agtgagttac ataaatagag caatttttaa ctgaaatacc
     2461 ttcttttgca caataataaa gggcattttg ttcagcgtga atggttctaa tacaatgtcc
     2521 atctctcatt tcatgaccaa cttcactaca atgcttatct ccagaaatac taccattata
     2581 tccagtagac actattctgt tttctgaatt gacaattata gctcctacat aagctctatc
     2641 acaagttcct ctttcagcta ctgtttcgca cagtctcata aaatattctt gccatgagca
     2701 tctattttca agttttttat tcaaatcata tacctccaaa atttatatct aatatataag
     2761 atttatacct taaaaataat attaaaaaca aatttatacg ataaataaaa tttattaata
     2821 ctgtttttag tctaaattat ttcattttaa attaaagtta caaggaatct aaaattatat
     2881 atatctttct tcgtaatatg aatgttacaa tataaaaaac tgtctcaata aaatagagac
     2941 agttttacag tcaaagttta taatacaagt tcatattttt ctttaaattc ttttacagct
     3001 tcgggccatg ccttttgttc tgtaatacct ttttctttag ctattttaat tgcaatctct
     3061 cttatcattg cagcgccttc taatattccc atttttaatc tcccttcaaa tatctttttg
     3121 tatagaatat aaatagtgct aagatttatt acatgctgca tatacaatat ctatacaaca
     3181 cataacttgt aaattccttt tattttcata cgtctttaac cgacatataa ttatattcaa
     3241 taagttttaa aattatgaac tcaattataa ttatttttat ttttttagaa tctaaacact
     3301 aaaaattgta ggtaaatttg tcttatgcct ttataaatat ttctacagtt ttaattactg
     3361 gacatatcta tttttattat aaaaagcgct taaaaatcga gaatatccgc ataaaaacta
     3421 tatataagca aaacgattaa aggaaaaaga agaaatttac ttgaatgtta aaatgcgttg
     3481 cttgaagcaa tgggcagttt tgcttataat atcggtaaca actgaaagaa aggtggttag
     3541 ccctaatgtt aagcgaaaac attaaaacaa tcaggaagtc aaaaggactt tcacaacaag
     3601 aacttgctat aaaactgaat gtagtgcgac aaacaatatc taaatgggaa caaggactgt
     3661 cggttcccga ttctgatatg ttgatttcca tatcagaggt gcttgaaacg cccgtaagca
     3721 ctttacttgg agaaactatt gttgagtcgg aagttgataa cttaaaagtg atttctgaaa
     3781 aattagagat tataaacttg caacttgcac aaagaaaaaa catgagaaga aaaatgtttc
     3841 attggttatt tattttatta tgtgcagtca tattaatagt ttttgtggtc ttagggagac
     3901 taaatagtcc ttacttggat tggaattata ccgaccctga aaccgctgtt tttggagtag
     3961 cttttcatgc ttttgagtgg ctatttgtca gattagtacc gattgtcctt ataggagcaa
     4021 ttattggaat ctttttgaca cgtaagaaaa tatgaagtaa ctctgctttt tataatacag
     4081 caagcgttcc ttaaaagcgt cattctacat ggcaatcgag gacgactatg taatgaagaa
     4141 cctttttgat ttccaactaa gcgaagttat tgaggacgac agtaggccca aaaccgcatt
     4201 aagtaaagta caggaagcaa gactgctgga cttatactct catgcgtcag atgcaggaaa
     4261 tgcgaggtct ggtagcatga tttacaacga tttgtacaac tattgcgagt agaaatatat
     4321 ggagataagt attatctaca aagtcagaaa agaaatgatt tgataattta cccatgttat
     4381 aattattgat gtgaaatcaa taaatcaaaa ttcctttttg ggatttaaca ataaggaaaa
     4441 agtgaatgac ttttgcaagg aggtgttaat atcagacaag taaagtcttt tgtttttcca
     4501 gttatatcct taatattctt agaccaaatt agcaaagttc ttataggatt attcttaatg
     4561 gactttgaaa ttgatataat tgggaaattt ttaagattca atcctgttca aaatacaaaa
     4621 ttaagtctct gctatctaac aaacggagca caaaaataaa aattaaatat aaagcacagt
     4681 taattttgat taaaaagttg actgtctttt cttttgtaga aatttaagca taggtaataa
     4741 agaaatgact gcatttaagc attatgtata taattggagg caataaaaag ccagcaaagc
     4801 ccataaaata gagctgtgct agcaacatga tgaattataa gaagataatc aaaaacttac
     4861 gctttatttt aatttaaaaa aatgattact gtaaagaaat cattttttat tataatcact
     4921 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
     4981 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
     5041 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
     5101 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
     5161 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
     5221 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
     5281 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
     5341 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
     5401 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
     5461 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
     5521 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
     5581 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
     5641 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
     5701 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
     5761 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
     5821 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
     5881 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
     5941 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
     6001 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
     6061 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
     6121 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
     6181 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
     6241 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
     6301 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
     6361 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
     6421 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
     6481 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
     6541 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
     6601 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
     6661 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
     6721 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
     6781 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
     6841 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
     6901 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
     6961 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
     7021 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
     7081 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
     7141 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
     7201 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgttta
     7261 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
     7321 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
     7381 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
     7441 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
     7501 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaacctcct
     7561 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
     7621 atgaatttat taattaggta aatttatata attctacttt ttataatata tgactattta
     7681 ttttatttct ttactattca agttctaaat caataataaa ttttgatatt taattgtatc
     7741 aggacaaatc tctaatttca tagattatta atataaaatc ataggttagt gtgaaaaagt
     7801 tgaaatttaa atattttaaa agataatttt tttgaactaa taggtataac aaaaatttat
     7861 agggggattg catatggaaa tcaatgttat agaaattttc cctaaagata aagctaaact
     7921 taataaaata gaaatggata aagctagttg gtttgtaaat ataataagta aaaaatatcc
     7981 taaagaagct ttaaatgaag catttagtac tttagaaaaa gaattaaata taagtaaagc
     8041 taatacataa gctttaatta ttgattttat atactattgg ttttatacac tatgacttga
     8101 cattatatta atcaaatagt atatttttaa atatagcaca atgaagtgtt aaaagagcaa
     8161 tggagctcca tagataaagt ctatggagct tttttgcgta tttaacttaa ggaggatagg
     8221 actttgaata actttgagat taaaactaga ataaaatttg gagaaggctc actaaaagct
     8281 ttgcaagaat taaaaaacaa aaatgtacta ataataacag acccttttat ggtgaaatca
     8341 aaaacaatag ataaaataac atctaattta ataaactcaa catatcaagt ttttgaagaa
     8401 atagtaccag acccaccaat agaagtagta gtagcaggaa tagaagcatt taaatctata
     8461 aatccagacg taataatagc attaggtggt ggttctgcaa tagatgcagc aaaatctata
     8521 atggactttt ctaaaaaaat attaaaaatt aacgaagtag agtttatagc aataccaaca
     8581 acaagtggaa caggttcaga agtaacatca tttgctgtaa taacagatag tcaaaaaaaa
     8641 tgtaagtatc cattagtgag tgatgattta cttccagata tggcaatact agacccagag
     8701 cttgtaaaaa ctgtaccaaa ttttataaca gcagatacag ggatggatgt attgacacat
     8761 gctatagaag cttacgtatc tacaaattct aatgactttt cagatgcact agctgaaaaa
     8821 gctataaagt tagtatttaa atacttaata aaagcatata aagatggaaa tgacatagaa
     8881 gctcgagaaa aaatgcacaa tgcatcttgt ttagcaggta tggcatttaa ccaagcatca
     8941 cttggcataa atcattctat tgcacatgta ttaggtggaa aattccatat accacatgga
     9001 agaacaaact ctatattact tcctcatgta atagaatata attcatcaat agtaggatat
     9061 actaacacaa cttactctgc tacagctaaa aaatatgcac aaatagcaga attagtagga
     9121 cttgggagtg ctaatataag aatatctgtt agaaatttag taaatgaaat aaagaaacta
     9181 caaaaagaaa tgaatatgcc aactaaattg acacaatgca aattaagttt agaagatata
     9241 actaatgaag aaagagaaat agcaaaactt gctataaaag atacttgtac attaactaat
     9301 cctaaggtac caactgaaga tgatataata aacatattaa agaacattaa ataactacat
     9361 tttattacat gtaaatataa tattatacaa atagggaaaa tgtttttaaa aatataaatt
     9421 atatattgac aaatataaaa cataaaacta taatgaaagt atcacaaagg cgtgatactt
     9481 tagtagaaag caatggagct tcttaggaat attaatatta ttcttatgga gctcctttta
     9541 ttttaatcaa atagcttttt aataaactat attcaaagaa agcagggaat aaatttgact
     9601 gaagaatcta agcaaagagt tattcaagaa tatgttcctg gaaaacaggt tacattagca
     9661 catatcatag ctaaccctaa tgaagacata tataagaagt taggattagt acttgataaa
     9721 aaagatgcaa taggaatact tacaataaca ccaagtgaag cgtcaataat agcagcagat
     9781 gttgctacaa aggcttcaaa tgtatcttta ggttttatag atagatttag tggttcagta
     9841 gtaatatcag gtgatgtaag ttctgtagaa tcagcgttaa atgatgtttt agaagtctta
     9901 ggaaatatgt taaacttctc atctacaaaa atcacaagga cataataata caatgaagaa
     9961 tataatattt atgggaaaga caggtagtgg taaaactacc ttatgtcaaa aactagacca
    10021 gttagaatta aagtataaaa aaactcaatc agtggaactt tataataatg caatagacac
    10081 tcctggtgag tatatggaaa atagaaacct atataatgcc ttaataacaa catcagcaga
    10141 tgctaaaatt atagcaatag tgtatgatgc cacacaagaa gaaaattata tagcaccagg
    10201 ttttgccagt atgttttgca aagacgtaat aggaattata actaaaataa ataaagctac
    10261 tgaaaacgaa gtcaatatag cttatgaaag actatcacta gcaggagcaa gtcaaatctt
    10321 taaaatagac acagttgata atattggact tgaaaaatta tttaaatatc tagaaaaata
    10381 taaaaatata gatgaagttt aaggggaaat atttttttat tcaaaaatat tattaaagta
    10441 tgataaaata aaaaaatgta taaactattt taagaagatt taatctttaa gcgaggtagg
    10501 aacatgggga aaaaaatact attggttgat gatgaaccac tgttaaggat ggatacaaaa
    10561 gatatattag aaagtcatgg ctatgaaata ataggcgaag caagtgatgg atttgaagct
    10621 gttgaaatgt gtaaaaaaca caaacctgat ttagttataa tggatataga gatgccaata
    10681 cttgatggta taaaggcagg caaaataata tgtaaagaaa atctagctgg tggagtactt
    10741 ttattaactt catttgaaga taaagaatac gtagaaaagg ctaaaagtat aggtgcttat
    10801 ggatatttag taaaaacagc aagtgaaaaa tcacttatcc caaccataga aatgtgttta
    10861 aataaggtaa atgaatttga aaaaatgaaa aaagatttag aaaaagtaaa ttctaaattg
    10921 aatgaaagaa aattagtaga aagagcaaaa ggtatactta taagagagtt taagatttct
    10981 gaggatgaag catacactag aataagaaaa ttaagtatgg ataaaagaac tacaatgatg
    11041 gaaattgcca aaatgattat aataggatac gatgactaat acgataaaaa aaatatcaac
    11101 tgcacaaaca tacttaaata ataacgaaat tgaaaaaatt atagaagtat caaaatcctt
    11161 agatttaatg gcttcttttt atgaagctga tgtttttata gatgtactaa gtaaatttga
    11221 tgatgaagca attgttgtgg cacatggtag acctaaagaa aagtcaatgt actgtgaaaa
    11281 tgttataggt aaaaaggctt taattgaaaa tgaaccagga gttataaagt gcttaaaaac
    11341 aggagaaaga gtaagagata ttaaagcttt aacacaggaa tataaactgg tcaaacaaaa
    11401 tattcagcca ataacactag atgacaaagt tttagcagta ctaatagttg aaaaagatat
    11461 aagtagtgaa gttaagaatg agtttaatgc tagtgaagac gaagaaaata aaaatttaca
    11521 tcagtttata aaacttatcg gaaataatga ctttttagtt gataatttaa attcagccat
    11581 attaatattt gataaaaatg gaaaattaaa aattaaaaat cgtattgcaa ctgagatata
    11641 caaaaatctt gggtttggat atgatataca agatatgcat tacaatgatt tagatataga
    11701 aaccaacacc tttgaaaata tattaatgga tgaaaattat gatgagacaa atgaagtttc
    11761 aataggtaat atgtttttta aaataaagac catatacatc aaagatgatg aatttaaggt
    11821 ttgcaaaata gtacaagata taagtgattt aaagagaaaa gaagaagaat tagttttgaa
    11881 aacagtggct ataagagaag ctcatcatag agtaaaaaat aatcttcata cagtaatatc
    11941 tataataaaa aaacaaagta gattatctca aaatgatgaa gtaaagcagt gtttagataa
    12001 cataataaat agggtatttg caatactttc gactcattat ttactatcta aagaagttga
    12061 taataatata tctatacgag aaggtatcaa tttattagtg tcaaatattc aaggtggcta
    12121 tttatttagt aaggatatag atatacgtat aactggtgaa gattttaaaa ttagtggaga
    12181 aaaagcaaca gcaatacttt tggttataaa tgaaatacta caaaattgtt atgaccatgc
    12241 attttcggat agagaacatg gtactataca aatattaata aataaagata acgactatag
    12301 aaatattgct attatagatg atggaattgg atttgatagt aataatataa ataaaaatag
    12361 cttagggaca tatataatag atagttatat aacacagatg ttaaaaggaa atatagagag
    12421 aaaaagtagt gagaatggta cagaagtatt aattactata cctacacaaa ttaatattta
    12481 aataataact catctacaaa gtagtagttt taattgagca aagaggctta aggttattaa
    12541 tagattaagt taattgaatg tatttataaa aattataagt atatattcaa tttaacaggt
    12601 atttattata actttaagcc tctttgcttg tttaagacat acttaaattt taggaggtga
    12661 gaaaatgaga gaagagttat taagtgttgg tattgacatt ggaacaagta ctactcagtt
    12721 aattttttct aaattaatag tagaaaatac tgaatcaaat ttttcggtac ctagaataag
    12781 tatagtagac aaagaaatta tttataaaag caaagtttat ttcacccctc ttatttcaaa
    12841 taatgaaata aatggtcaac aaataaaaga tatagttgaa aatgaatata aaaaagcaaa
    12901 tatatctaaa gaagatatac atacaggagc agtaataata actggtgaaa cagctagaaa
    12961 agaaaatgca aatgatgtac ttcatacttt gagtggattt gctggggact tcgttgttgc
    13021 aacagcagga cctgatttag aaagtataat atcaggaaaa ggagctggag ctcatattta
    13081 ttcaaaagaa cattcaacta caattgtaaa tatagacata ggtgggggaa ctagtaacat
    13141 agcctcattt aaaaggggag aagtaaccga tactgggtgt ttggatatag gtggaaggct
    13201 tgtaaagata gataaaataa gtaaaaaaat aacttatata tcacctaaaa tagataaaat
    13261 cataaaagat aataatttaa atcttcaagt aggcacaatg gctactgtag aaaatacaaa
    13321 gccagtagtg gatataatgg ttgacttatt aatggaaagc ataggactaa agaaacagac
    13381 acctttgttt gattttataa taaccaataa atcaataagt atagatgaaa ctattaaaaa
    13441 tgtttcattc tctggtggag tagctgatta tatttactat gatggagaaa taaaagatta
    13501 ttttgaatat ggtgacttgg gaattttact tgggcaagca ataaaaaatt gtgaatacct
    13561 taaaaaagta aatgtagtta aatctatgga aacaataaga gccacagtgg ttggagcagg
    13621 ttctcatact actgaagtaa gtggaagtac aataacttac actagcgaaa agtttccact
    13681 taagaacttg ccagttttaa aattatcaat tgatgaagaa acaggcactt caaaacaaat
    13741 cagtgaagct ataaaaaata aacttaattg gtttaaacta gaaaatgatt tacagaaagt
    13801 tgctatagcc atgataggaa agaaaaatcc aaccttttta gatatacaag agtatgctaa
    13861 agggtttgta gatggaatgc aacaaatcat agaaaatgat ttacctttta tagtaattgt
    13921 tgaaaatgat atggcaaaag tgttaggtca agcaatatat gcattattaa attatgaaaa
    13981 agaaattgtt tgtatagaca gtgtcaaggt tgaaaatgga gactatatcg acattggaaa
    14041 acctatagtt gaaggtaaag ttttacctgt ggtaataaaa acattagttt ttaattaata
    14101 gaaggaggta agtaaatgat tttaaataca aaattatttg gacgtactta tcagtttaaa
    14161 ggcttatatg atgtaatggc taaagctaat gaagaaaaat caggagataa attagcaggt
    14221 cttgcagcag aatcagctga agaaagaata gcagcaaaag ttgttttatc acacataaaa
    14281 ttggaggata taagaaataa tcctgcagtg gcatatgaag aagatgaagt aacaagaata
    14341 atacaagatg ctgtaaatga aaaagtttat gaagaaataa aaaattggac agtctctgaa
    14401 cttagagaat ggctattgga ttcaaatact acaagtcttg atataagaag aatatcaaga
    14461 ggattaactt ctgaaatgat agcagcagtt gcaaaattaa tgtcaaatat ggacttagtt
    14521 tatgcagcta aaaagataaa ggttactgct cactgtaata caactatagg agaacaagga
    14581 acattatcag caagacttca acctaatcat ccaacagacg acccagatgg aatacttgca
    14641 tcacttcttg aaggtttaac ttttggtata ggagatgcag tacttggatt aaatccagtt
    14701 gatgactctg ttgagagtgt tactaaagta ttaaaaagat ttgaagaagt aaaacaaaaa
    14761 tatcaaatac caactcaaac atgtgtattg gcacacgtta ctacacaaat ggaagcaata
    14821 agaaatggtg ctcctgcaga tttaatattc caatctatag caggttctga aaaaggaaat
    14881 gaagcatttg gatttaacgg cgacactata gaagaagcta gacagttagc cttaaaacac
    14941 ggtacagcta caggacctaa cgttatgtat tttgaaactg gacaaggttc agaattatct
    15001 tcagaagcac atcatggaat agaccaagtt actatggaag ctagatgcta tggatttgct
    15061 aaaaagtatg aaccattctt agtaaataca gttgttgggt ttataggacc tgagtactta
    15121 tatgatgcta gagaagttgt aagagcagga cttgaagacc actttatggg taaactacat
    15181 ggcttaccaa tgggtgttga tgtttgttac actaaccata tgaaggctga ccaaaatgat
    15241 atggaaaact tagccatact attaacaaca gcaggatgta cgtattttat gggaatacct
    15301 catggagatg atgttatgct taactatcaa acaacaggat accatgaaac agcatcactt
    15361 agagaaatgt ttggactaac agcaataaaa gaatttgatg cttggatgga aaagatggga
    15421 ttctctaaag atggtaagtt aacagagttg gcgggagacg catctgtatt attaggatag
    15481 gaggtattta atatgaatga gaaggattta aaagcattgg tagaacaatt agttggacaa
    15541 atggttggag aattagatac taatgttgtg tctgagactg ttaaaaaggc aactgaagtt
    15601 gtagtagaca acaatgcttg tatagatgat ataacagaag ttgatataag aaagcaatta
    15661 ctagtaaaaa atcctaagga tgcagaagct tatttagata tgaaagcaaa aacacctgca
    15721 agattaggta taggaagagc tggtactaga tataaaacag aaacagtttt aagatttaga
    15781 gcagaccatg ctgctgcaca agatgctgta ttctcttatg tagatgaaga atttataaaa
    15841 gaaaataata tgtttgcagt ggaaacttta tgtaaagata aagatgaata cttaacaaga
    15901 ccagatttgg gaagaaaatt ttctccagaa actataaata atataaaatc taaatttggt
    15961 acaaatcaaa aggttttaat attagttgga gatgggctta gctcagctgc aatagaggca
    16021 aatttaaaag actgtgtgcc agctataaaa cagggtctaa aaatgtatgg tatagattca
    16081 agtgaaattt tatttgttaa acattgtaga gttggtgcta tggaccactt aggtgaagaa
    16141 ttaggttgtg aagttatatg tatgttggtt ggagaaagac caggattagt tactgctgaa
    16201 tcaatgtcag catatatagc ttataagcca tatataggaa tggcagaagc aaaaagaaca
    16261 gttatatcaa acattcacaa aggtggaaca actgcagttg aagcaggtgc tcatatagct
    16321 gaacttataa aaactatgtt agataaaaaa gcttcaggaa tagaccttaa ataaagtcga
    16381 gggagtgtag aatatgaaaa atgatgtaat tcgtcctaat atactaggaa ttaaaataat
    16441 ttcaaatata agtccagaaa tggcacaaaa actagaatta aaatcacatc acaaaagctt
    16501 aggcttaata actgcagact gtgatgatgt tacgtataca gctcttgacg aagcaactaa
    16561 agcttcagaa gttgatgttg tatatgcaaa atcaatgtat gcaggtgctg caaatgcttc
    16621 aacaaaactt gctggtgaag tattaggaat aatagcaggt ccaagcccag cagaagtaag
    16681 aagtggtcta aatgcagtag ttgattttct agagtatgga gcaactttta taagtgcaaa
    16741 tgatgatgat tcaatagcat actatgctca ctgtgtatca agaacaggta catacctttc
    16801 agaggtagca ggaataagag aaggtgaagc attagcttat ttagtagcac caccacttga
    16861 agcaatgtat gcactagatg cagcaatgaa agctgcagat gttaagatgt gtgaattatt
    16921 tgcacctcca actgaaacaa actttggtgg agcattactt acaggttctc aatcggcttg
    16981 taaagcagca tgtgatgcat ttgcagaagc agtaaaatca gttgcagaca atccaacagg
    17041 attttaagga tagttagatg gatgctatag gcttaataga agtaatagga tatgttgctg
    17101 caatagaagc ttctgatgct tgtttaaaat ctgcaaatgt taatattgta agagttgata
    17161 aggttggagc aggtattgtt actttaacaa tatgtggaga tgtcggtgct gtaaaagctg
    17221 ctttagaagc aggtgaaata gcagcttcca gagttggtac attaagaaca tcacatatta
    17281 taccgagaat ccataatgaa gtaacaaatg ctttattcaa aactaaagaa cctaaagtct
    17341 gtgaagtttc agaagataaa gaaaaaaact ttgagattac attggaaata gctgataatg
    17401 tatcgaaaaa tttatcagaa aaagaaaatg taattttaga tgtcaatgaa aacagtggag
    17461 atactgaaaa tttatatagt gatatagata aaacaaacaa aaaaattgaa acagttgatg
    17521 atagtttaac agaagctaat gaggaaaaaa tagaaaacac taatgataaa gaagtattgg
    17581 aacaagttaa aatagaaaat tccgattttg atagagatga cctatcaaag aacagtgtaa
    17641 aaaaacttaa agctatggct aagaaattag attcttcaat aacttataaa gatttaaatg
    17701 ttcttaaaaa agaagaatta ataaacttag ttaaaaagct tagtagaagg gatagatagt
    17761 tatgacactt ttagataaag atttagtatc tatacaagaa gtaagaactc ttgtaagaag
    17821 atctaaagtg gctcaagaga aactttctaa aatgagtcaa gagcaaatag ataaaatagt
    17881 aaaagctgtt gtagatgcag catttaataa tgctactaaa ttggctaaaa tggcaaatga
    17941 agaaactgga tttggaaaat gggaagataa agtaattaaa aatacttttg catctatggg
    18001 agtttataaa actttaaaga acacaaaaac tattggaata attggtgaag ataaagaagc
    18061 caatacgata gatatagcta caccagttgg agttgttgca ggtcttatac cttcaacaaa
    18121 cccaacatca acagtaatat acaaaactat aatatctcta aaagcaggaa atactatagt
    18181 tttctctcca catccaaatg caaaaaactg catacttgaa acagttaata taataagtga
    18241 ggctgctata aaggctggag ctccagagga ttcaatatta tgtataacta atccaagtat
    18301 agttggaacc aatgaattga tgaaacataa agatacaaac ttaatactag caactggagg
    18361 agaagcaatg gttagagctg cttactcttc aggaacacca gctattggag ttggaccagg
    18421 aaatggacca gcatttatag ataaatcagc aaatgttaaa ttagcaatca aaagaataat
    18481 agattctaaa acttttgata atggtgttat atgtgcatca gaacaatcta tagtaataga
    18541 aaaatctatg gaagaaactg ttttaaaaga gcttaaaaat caaggtgcat atgttttaaa
    18601 tgaagaggaa agtgaaagat tagcaaaatt catattaaga gctaatggta ctatgaatcc
    18661 acaaatagtt ggtaaaagtg ttgaggtaat agcaaattta gcagacttaa caaatgtacc
    18721 taaaaataca aaagttctag ttgcaagaga aaataaagtt ggtcacaatg tagcctattc
    18781 aagagaaaaa cttacaccaa tactagcgtt ctatgttgaa gaaaatgtag aagctattat
    18841 gaacagatgt agagaaatat tgttaaatga aggtgcaggt catacattct caatgcatgt
    18901 aaatgatgaa gaattggtaa aaagatttgc ccttaatatg ccagtatcaa gaatacttgt
    18961 gaatactcct tcagcacttg gaggaatagg tgcatcaaca aaccttgtac cagcactaac
    19021 acttggttgt ggtgcaatag gtggaagttc aacttcaaac aatataggac cacttgattt
    19081 aataaatgtt aaaagagtag cttatggaat caaagaaata gaagaacttg gaagagataa
    19141 atctcaagtt gaaaaatcaa tgtttgactc aatggataaa gaagaattaa taaatcttct
    19201 tgttcaaaaa ctagtcaaag aactagcata actaaaataa acaatgaaac ttatggctat
    19261 cgctataaat ttctaaaaat ataaactata aaaaactata aaattaaatt tttggaggat
    19321 aagattatgg caagtgcaaa cgcattagga atgatagaaa ctaaaggatt agtaggagca
    19381 atagaagcag cagatgcaat ggttaaagct gcaaatgtac aattagtagg aaaagagcaa
    19441 gtaggtggag gattagttac tgtaatggta agaggagatg ttggagctgt taaagctgct
    19501 acagatgcag gagctgccgc tgccgagaga gttggagaac taatatcagt tcatgttata
    19561 ccaagaccac actttgaagt agatgctata ttaccaaagg tatcagctga gtaatataag
    19621 aataattata aaatttaaat tttgattgaa aaagaggcta tctcaaaaat gagtcttact
    19681 aataaactca atgtattttg aggtagtctt ttttatacat ctaataggag atgtgataaa
    19741 atgggtgttt taacagaaaa cgaagtaaaa agaaaaattg gaaaaaacaa aaataatata
    19801 actgaatttt atatagaaaa aggtcaaata ataactcctt ctgcaaaatc ttatttagct
    19861 gagagaagta ttgagcttaa atatgttgat aaactttcag atgtaaacaa tgaacctaaa
    19921 gaagttgtaa aagaagtagt taaagagaaa aaatataaat atactactgt atttggcgcc
    19981 aaattggatg aaaaaccaga acatatgact caattaaatg gaaatctttt agtttttaaa
    20041 gaccataaaa gaataatatt gagaggaaaa atagattctc ttgaatcaga aatactaaag
    20101 gtacagatac tttgtcaaaa actagatatg ccaaagcttg ttaaggaatt gcaagaagta
    20161 ttagactttg taagaaatat aatgagatgt gaagttttag atgaaaagct aggggacttt
    20221 aaacttttag gattaactcc tcaagaactt agagaacaat ctcactatcc taaaaaatac
    20281 tttggtatag gacatgagtt cccaagttat gaaatgggag aagtagtagt agctataaat
    20341 agtataagaa gtaatacacg agaggttgaa cttgcagcct atgaagcatt taagggtgag
    20401 tatggaaatg ttcacagaga agatttaata aaggcactta atagattatc gtcagtattt
    20461 tggataatga tatataaggt tagaactaat aagtataagt agttggagga cataatggag
    20521 aatctattaa atgaaatagt ggaagaagta ataactagag ttaaaaaaga agcatttata
    20581 gaagttgaag catcaggaag acacgttcac ttaagtagag aagatgtcga taaattattt
    20641 ggagaaggat atactcttac aaaactaaaa gacctatctc aaccaggtca atatgcatgt
    20701 aaggaaagag ttacaataac tggtcctaaa ggaagtataa aaaatgtagt tgtacttgga
    20761 ccatgtagaa atgaaactca ggtagaaata tctttaacag atggttcaac tcttggacta
    20821 aaagctccta taaaacaaag tggagactta gaaggtactt taagtattaa aatatcaaca
    20881 caatatggtg aagttgaact tgataagggg ctaatggttg ctaaaagaca tatacacatg
    20941 actcctaaag atgctgaaaa attcaatgta tgtgataaag agatagttga agctaaggtg
    21001 atgggtcaaa gacctttaac atttgatgat gtagtaataa gagtgagtga cagttttaaa
    21061 acttatatgc atatagacta tgatgaagcc aatgcatgtg gttattcaaa aggaactgtt
    21121 gctaaaataa taaagaaagc ataaatttaa cctactatat aaattacctc tagggaggat
    21181 ttaatatgaa ttatgataac cttgtagatt taataacgga agaaatctat aaaaaaataa
    21241 atagtaatga aataaaaata tctaataaac ctaaagctgt gattgtattt gaacaagata
    21301 acaataaatt taacttatta aaagatgaat ttgaaatagt agaatttgat aaaagtataa
    21361 gggaatgtga aatagtgata gtttcaagac tttgtatgag ggggctatcg aatatagcac
    21421 ttggaaattc aacaagtgat gaagaaagat ttatactaaa aatgattatg aagggcaaaa
    21481 aagtatatgt cctagatgaa ggaattgaat acaaaaaata taaggatact gcaccaaaag
    21541 cattatataa gaaatttatg tcatttgaag atgaaatatg taaatttggt gtagaaatta
    21601 taaaagactt aaatgttata actaaaaata agttaaatat aaagaaagaa ctagaaagta
    21661 ctagaggttc taaaaaagaa gatacaaaag ttttagataa agatgtttta aatttaacca
    21721 gtgtcgaaag tgtaaaaata gataatgaat ttagtttaga tttaaggaat aaaaaactta
    21781 tatcggaagc tgacttaaga aaaccaacta taaatggagt taaaaacata ttagttaata
    21841 aaaaaagtat aataacacca ttagctgtag attttatgag aatacaccat ctcaaactaa
    21901 agaaactgta aggaggtaat tttatggata taggaaaagt tgtaggtaat gtttgggcta
    21961 caagaaaaga tgaaaaatta tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta
    22021 aagacacata caaggaaggc ttcatggttg cagccgataa tgcaggagca ggaaatggag
    22081 acttagttct tatatccaaa ggttcctcag ctagagagtc gattgaaaaa tctcatgttc
    22141 cagtagatgc aactatagtt ggagtagtcg attcgtttga ggtgtttgat gaataaaagt
    22201 ataggagcta tagagtttaa aagcattgct aaaggtatag aagtatccaa tgaaatgata
    22261 aagaaatctt cagttgatgt attgtactta aaaagtattt gcccaggtaa atttctaata
    22321 atagtaggtg gagaaacttc ttatataaat gaatgtgtag attatggaat aaaacttgga
    22381 gaaggatata ttgtagataa cttcgtaata aatgcaattt cacaagaaat attagatgga
    22441 tttaaaaata agtatcaaaa gctagatagt atagtctcta taggcgttgt tgaaagtagt
    22501 aaagtatgcg ctggaataaa aatgcttgat aagactttaa aatctggcga cttagtatta
    22561 gtaaaacttc aattatcttt tgctataggt ggcaaattag tatatatagt agcaggagat
    22621 ttaagtagtt tagaatatgc attaaaagaa agtgaaaatg tagtaagaga aaaagaagta
    22681 atatataaga ctgtaatacc atcagtagac agtcaaataa taaaaagtct aataaaataa
    22741 tggggggaat tatgagtata aacgaaatta taatctatgt aatggtagtc ttcatggtac
    22801 ttggagctat agataaatgt ataggtaaca aatttggtct tggagaacaa tttgaagaag
    22861 gtataatggc aatggggtca cttgcagttg caatggttgg tgttatatgt ttagcaccag
    22921 ttcttgctga tgtattaaga ccaattgtag taccagtatt taatatactt ggagctgacc
    22981 cagctatgtt tgctggaagt ttattagcta atgatatggg tggtgcgcca cttgcacttg
    23041 aacttgcgaa agaccctaat gctgggttat ttggtggatt aatagtaggg gctatgatgg
    23101 gacctacaat agtatttata ataccagttg cgcttggaat aattgaaaaa gaagaccaaa
    23161 aattcttagc aacaggaata cttgcaggta taataacaat acctattgga gcattcgttg
    23221 gtggattagt tgcaggattc gaggtaatga tggttcttag aaacttagtg ccaataataa
    23281 tatttgcagt agttatagca cttggattat taaaattcga aaatgctatg ataaaaggat
    23341 tcacatactt tggaaaaggt gtagtaatag ttataacttt aggacttgct gctgccatag
    23401 ttgaagcttt aacaggaata gttgtaatac ctggaatgaa accaatcagt gaaggtatag
    23461 aaatagttgg agatatagca atagttttag caggagcatt cccattagtg tttgttataa
    23521 caaaggtatt taataaacca ttaatggctt taggaaaagt tcttggaatg aatgatgtat
    23581 cagcagcagg ccttgttgca agtttagcta actgtatacc aatgtttggt atgatgaaag
    23641 atatggacaa tagaggaaaa ataataaatg tagcattctc tgtatcagca gcattcgtat
    23701 ttggagacca cttaggattt acggcaggat ttaattctaa tatgataaca ccaatgatag
    23761 ttgctaaatt agttggtggt ataacagcag tattgttagc tatggttatc gcaaataaga
    23821 cactaaagaa ggagaatgta taagatggat atatcaaata tagataaaaa tttaattgaa
    23881 acattagtaa gacaaataat agaagaaaaa ataagtggaa ctaaagacac agtagatttt
    23941 gttagaaata aagacattag tggaataact tctataaagt taccaactgt aaaagttagt
    24001 gaatctgata gattagatac agggaatcca agtgatgtag tttacacaaa agatttattt
    24061 acattagagg aaagcccaag attaggttgt ggtatgatgg aaatgaaaga aacaactttt
    24121 gattggactc taaactatga tgaaatagat tatgtaatag atggaacttt agatataata
    24181 atagatggaa gaaaggtttc tgcttcttca ggtgaattaa tattcatacc aaaaggaagt
    24241 aagatacaat tttctgtacc tgattatgct agatttatat atgtaacata tccggcagat
    24301 tgggcatctc aaaattaata aaaatcaagt taagttaata ataatcaaat aaataattta
    24361 aaatacaaac tcttaaataa gcacacatat ttaaaaaagc tatgaattaa aattcatagc
    24421 tttttttata aagaatgtta tattttagac gtattttttc tatatttact gg
//
LOCUS       DAKBNN010000040        24425 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000040 DAKBNN010000000
VERSION     DAKBNN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.040"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            72..977
                     /locus_tag="OUB02_001392"
     CDS             72..977
                     /locus_tag="OUB02_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885159.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            1092..1272
                     /gene="ssrS"
                     /locus_tag="OUB02_001393"
     ncRNA           1092..1272
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OUB02_001393"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1455..2672
                     /gene="cdeC"
                     /locus_tag="OUB02_001394"
     CDS             1455..2672
                     /gene="cdeC"
                     /locus_tag="OUB02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HCT8885160.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(2772..4094)
                     /locus_tag="OUB02_001395"
     CDS             complement(2772..4094)
                     /locus_tag="OUB02_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCT8885161.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEIALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(4145..4939)
                     /locus_tag="OUB02_001396"
     CDS             complement(4145..4939)
                     /locus_tag="OUB02_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HCT8885162.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            5049..6494
                     /locus_tag="OUB02_001397"
     CDS             5049..6494
                     /locus_tag="OUB02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT8885163.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            6523..6732
                     /locus_tag="OUB02_001398"
     CDS             6523..6732
                     /locus_tag="OUB02_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCT8885164.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            6752..8146
                     /locus_tag="OUB02_001399"
     CDS             6752..8146
                     /locus_tag="OUB02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCT8885165.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKGI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            8386..9138
                     /locus_tag="OUB02_001400"
     CDS             8386..9138
                     /locus_tag="OUB02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8885166.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            9164..9577
                     /locus_tag="OUB02_001401"
     CDS             9164..9577
                     /locus_tag="OUB02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8885167.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            9581..10324
                     /locus_tag="OUB02_001402"
     CDS             9581..10324
                     /locus_tag="OUB02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HCT8885168.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKHRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            10324..10794
                     /locus_tag="OUB02_001403"
     CDS             10324..10794
                     /locus_tag="OUB02_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885169.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            10811..11569
                     /locus_tag="OUB02_001404"
     CDS             10811..11569
                     /locus_tag="OUB02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8885170.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            11553..12395
                     /locus_tag="OUB02_001405"
     CDS             11553..12395
                     /locus_tag="OUB02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT8885171.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(12555..13460)
                     /locus_tag="OUB02_001406"
     CDS             complement(12555..13460)
                     /locus_tag="OUB02_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8885172.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            13822..14556
                     /locus_tag="OUB02_001407"
     CDS             13822..14556
                     /locus_tag="OUB02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885173.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            14767..15948
                     /locus_tag="OUB02_001408"
     CDS             14767..15948
                     /locus_tag="OUB02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCT8885174.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASIG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEHLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            15932..16504
                     /gene="mobB"
                     /locus_tag="OUB02_001409"
     CDS             15932..16504
                     /gene="mobB"
                     /locus_tag="OUB02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCT8885175.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            16623..16898
                     /locus_tag="OUB02_001410"
     CDS             16623..16898
                     /locus_tag="OUB02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885176.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            17141..18841
                     /locus_tag="OUB02_001411"
     CDS             17141..18841
                     /locus_tag="OUB02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885177.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            19204..20391
                     /locus_tag="OUB02_001412"
     CDS             19204..20391
                     /locus_tag="OUB02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885178.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            20451..21614
                     /locus_tag="OUB02_001413"
     CDS             20451..21614
                     /locus_tag="OUB02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8885179.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDVLEIGTALYVQYAVDFLNK"
     gene            21765..22568
                     /gene="zupT"
                     /locus_tag="OUB02_001414"
     CDS             21765..22568
                     /gene="zupT"
                     /locus_tag="OUB02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCT8885180.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            22664..23224
                     /locus_tag="OUB02_001415"
     CDS             22664..23224
                     /locus_tag="OUB02_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HCT8885181.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            23474..24187
                     /gene="rgbR"
                     /locus_tag="OUB02_001416"
     CDS             23474..24187
                     /gene="rgbR"
                     /locus_tag="OUB02_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HCT8885182.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         9624 a         2582 c         4322 g         7897 t
ORIGIN      
        1 aaaatggaac attaaaatat gaaaaacata ctttaatata gggcaattat ataatataag
       61 ggggaattgt cttgaataac ttactatttt tgattggagt gattgcattg agtaatggta
      121 atatctctat atttgatgat aaaaataaat caaaaggaaa taatagagtt agaaaaaaca
      181 aaaaaaatgc aaatgataaa ctttcacagt ctacacatgg caatttaagg tttgatgcaa
      241 atgatataaa aagaggttta aggctcatgg atttaagtga agaagattta gaaatgggaa
      301 tggaaataat aacaagaact aaaaaataca tgtcaagaga tgaaagaaaa atactaataa
      361 aaatagaaag tgttttagat ttagttagag gaataaaaaa attaaataat atagatatgg
      421 tagatacaga agatgaaacg gattttttca gaaaaatgga tagtgatgat aaaaagaata
      481 tgatgataaa ggaaattata gatgtattcc cagaaaaaag aaaaaattca gttgaaaaag
      541 ctataggaat gaaaaagaag atagatttat ttgcagaatt atttttacca gatgactttg
      601 gtgaaggtgg atttagtttg agctctttgg caaatataaa caatcttggt agtatgaata
      661 atctcaaatt gttaggaagt cttttaagag gtgatgatag taataatgat gatgaagaca
      721 ataatgatga gttaaattat gaagacgaag aattagattg tgaagacaaa gaattggatg
      781 atgatgagtt aacagatgaa ggtgaaatgg aggataatga agatgaatta gtagaagata
      841 agaacacagg ctatgaaaat atagaaaatg atgaagatat agtatacgat gaggaactat
      901 acaacattaa tgagcaagaa tccgaataca attataatga aaaaaatact aaaactagag
      961 ctaagaaaaa acgataatta actaaataca taaagaaaaa aggaagaaaa agtaatgata
     1021 gaataagatt atctcaatta ctatataaaa ccactttaaa aaattcaaaa tttattatat
     1081 aataataata tgaaatcctg aaacatacga taaggctttt ttaaattcaa cctacaaatg
     1141 aaatacggga gaccgtgtct ggtaaattat aacatgccta gttagtttgg acgttaaagg
     1201 ttactggcaa gtcacccacc tgggagtgcc tagggtgtga atttacttag ccaccggtgt
     1261 ttcgggacaa aaaaagtgct actatttcaa atagtagtac tttttttatt aatatgcatt
     1321 tttctactaa atacgcctaa ttattgataa attcaaaaag caagtaacaa ttaagattat
     1381 aaaagcatag tattaattag gcttcaaaaa gccaaagact taataagaat cgcttacagg
     1441 gagggcaatg aaaaatgcaa gattataaaa aaaataaaag aagaatgatg aatcagccaa
     1501 tgtctacaat gaatgaagaa gaagtgtata cagatgaaat aaattcagaa gacatgagag
     1561 gttttaaaaa atcacaccat cataatggat gtaatactga taataagtgt gagtgccatg
     1621 atgattgcaa tccatgcaac ccatgtaatc catgtaaacc taacccatgc aatccatgca
     1681 aacctaatcc atgtgatgac aattgtggat gccatgacaa ttgtaaatgt gattgtgaac
     1741 catgtgaaat ggattcagat gaatgttttg aaaacaaatg tggaccagaa tgctgtaatc
     1801 ctatatctcc aagaaacttc tctgtatcaa atgcagtgcc atttgcaata gaggctaata
     1861 gaatatttga tactatgcaa ttccaaacat ttacagatgc aacaggacca aatggagagc
     1921 cattaacttt tgaaacagaa gtagtagaag tatttggttc agttccaagt gcaggtcaag
     1981 caagtgtaac tatagaaaaa atatgcttaa gtaatgatgg aatcgttata gacacaggaa
     2041 tgacaacttt agaagatttc gatttagacc cattaggaga tatagtagga agaaactgtg
     2101 aaacaacttt tgaatttgca gtttgtggag aaagaaactc tgagtgctgt agacaaggaa
     2161 aaggcaaatc agtagcttat aaacaaagag gattaactgt agcagttcgt aatttagtac
     2221 tagagctaag aggtagatgt ggatgtacag agttcgttgc attagctttc ccagcagtta
     2281 gagcaggagg tggatgtaag agaagagttg attatgtaga atttactttt aacacacttt
     2341 cagcaccaat atgcttgcca gctgacggaa gagctgttac tttaagacaa gaatatcaaa
     2401 ctaacttaac tgtagattgt ataggaaaat ctatattaaa attagaatgc aacgaatgtt
     2461 gtgaaccttt ctatgaatta attataccaa atgatataga tttagtactt tgcttacaag
     2521 aaacagttag cacattaata agtgaacaaa tagtagtttt agcatcacca aatccaatcc
     2581 aaccaagact tgttgatact ttctctaaag tatgtgattt ttcgcaatgt ggacctaatc
     2641 atggaagtgg aaagccaagt tgccacagat agataggtag atatatagac agtttaaatt
     2701 ataaataaaa atcatctaag gaaggctacc aaagtgtata atttacattg gtagccttca
     2761 cgtttttata actagcgtat agattttagt gatttaaaaa gcactatagt atgtaataga
     2821 caagtaaaga ttgatactat cgtcattgtg tatatgtatg cataaatatt aagtcctgga
     2881 attggaagaa atacatataa agctattagt tgaattaaca tgccagtaat agtatttata
     2941 cttgaagcta attcttttct tattgaatgt aaaatgccag atagagtttg atttagggac
     3001 ataaatagtg gaactaaaaa catagctttt agatattctc ctgcaagtgt atttttaaat
     3061 actaatatac ataaatcttt tccaaagaaa taaaaaaata ttgatgatag tatgcctaca
     3121 aataaagtaa gaagtatgga gtatttgatt tttttaatta cactcttacg ctttcttaga
     3181 gcaatttcct gtgaaatagt tggtattaaa tttacaataa gcgctgaacc aacagtaaaa
     3241 ggtaaaaata taaaaggcat taccatccca ctgacaatac catacatact taaagattgt
     3301 tgataagtca ttcctgataa tgctaatctt gatggtatca tcatagaact tatagattgc
     3361 aaaagtatgt ttgacattct attacaagtt atgggaatag acatttttaa tgtttccata
     3421 gaagaattat aaaaatcttt tatgcttata atatacctat tgtcaaatga tgaatctcgc
     3481 cataagcaaa taagcataaa tataatatta ctcaactcac caattgatat acctaataga
     3541 gctatataac aattcataga tttattattt atgtacataa caagtaaaaa aacaaataat
     3601 attttaccaa tttgttcaat gatttgtcca atagcaggga tttttacatt ctttatgcca
     3661 tagtaatatc ctcttagtac gtttgaaaga gttattataa ctatagcagg acaaacagct
     3721 agtataaata gatttaattt agcatctttt aaaaatttaa gtgataaaaa agaactattg
     3781 aaagatgcaa ctatagatat aattagtgct acgaaaaatg atatatataa agttgatata
     3841 aataaagcat tagtattgtg tctatcgttt aaagcttttc tctttgcaac tagacaacta
     3901 agtgcagtag gtatacctgt tgttgtaaca gctaagcaaa tcatcaaaaa gttaaaaatc
     3961 atatgataga tacctaaacc tgtttcacct agtgctcttg ataagaatat tttatataaa
     4021 aaaccaaata ttcttacaat aaaattggat ataaataaaa taactgttga tagtactaaa
     4081 taaggtactt tcaaaaaaat cacctcctta tcttttaaat atattcacaa ggaggtgtaa
     4141 aaatttataa ttttataaat tctggtttca atttattaaa tttagataca tatttaaatc
     4201 ccatattttt tagtaatgaa tatatgtagt caaactcaca tgcaacttga cttatatgat
     4261 gtgaatctga acctgtggtt atgatttccc ctcccaagtc tttatacatt tgaagtatat
     4321 acttagatgg ggttaggtct ggaagattat atctataaca agaggtattt atctcgatac
     4381 ctttgccatc ataaatagct tgtttaagag tttcttcaat tatatcagaa aatagtctat
     4441 catctaaaat agtgtcataa ggtgcatacc ttttaatcaa atctaaatga cctaagacag
     4501 agtaatcttt ataattctta acaatattat ataaataaag atagtaattt tcataaactt
     4561 catgttgagt tttatcttta aaataattac caaggtataa gtccattgta tctatagcat
     4621 ggatagaaca tattataaag tccaatggat attgatgtgc ttcctttttg tatgtatcca
     4681 ttaattgagt ttgaactcca aattcaattc ctttttttat tgatatttta tctttatatt
     4741 tgctttgaag agtctctaaa ctcttaaaat aatcttcata gacaattgaa aaagaatcat
     4801 cagcatatac atcatagtca acatggtctg tgaaacaaat ttcctccaaa ccgagttcaa
     4861 tagatttttt taccatttct tccattgtag attttccgtc tgtagagaaa cttgagtgca
     4921 tgtgatagtc gtaaaacata aatatttccc ctttttataa aaattttggt attatatata
     4981 catactaaat aatatcaaat atacataaaa gcatcaataa aatataaata caatgagcgg
     5041 tgataaattt ggaaaaatac agaatagatt taaatgagaa aaaatcaact aaatatatac
     5101 agatatttaa tcatataaaa gaattgataa taaataaaga gttagtagaa catgaaaaat
     5161 tgcctccaat aagaaaacta tcaaattttt tacaagtaaa taacactact atagttaagg
     5221 tatacgagct tttagaaaat gaaggatatg tatataaaat tatagggagt ggtacttttg
     5281 tatcaagttt aaattcttat aaaagcggta tagataacag gaaaaataag attattgtgc
     5341 aaagagaaga tttaattcat tttgacaatg ggaatccatc taatgatatg tttcctatcg
     5401 attcatttaa aaatgctgta aatatggcat tatctaaaga tggtagttca atatttgaat
     5461 atgatgaagg tcttggctct gaagaattaa gagaaaaaat ggtagagtat ctaagtgatg
     5521 gaaatataaa aactactaag gaaaatattc aaataatatc aggtgctcaa caaggtatag
     5581 atatagtttg taaaggactt ataagttatt cagatgttgt ttttatggaa gaaccttctt
     5641 acaatggagc aattgaagtg tttaaaagta gaggtgcaaa aataatatct atacctatgt
     5701 tagatgatgg aattgatata ggtattttaa aattaaagtt ggaaaaaata aaaccaagac
     5761 ttatatacat tatgcctaat tttcaaaatc caactggaat atcttactca acatataaaa
     5821 aaaagaaatt gatagaattg gcagaagaac atgattttta tataatagaa gatgatttta
     5881 taagtgattt tgtttttgaa tcaaaggaca atagaacaat tagaagttat gatgataaaa
     5941 atagagtagt atatataaaa agcttttcaa aaatacttat gccaggtctt aggataggaa
     6001 ttgtagaaat gccaaatgag ttgttaaaaa aagttttatg ggctaaatat tcatcagata
     6061 tatcaacacc aggtttaatt caaaaatcaa tgtattacta tatgaataat tttgattgga
     6121 agaattattt gagtcatata gagaagatat atactgataa gtataaattg gcaaaaaact
     6181 tgattaatga caaattgagt ggtaaattaa aagttagaaa atcttgtgga ggaataaact
     6241 tttttttaga attaccaaga ggatatacat ctcaagcatt tacaaatttt atgttaaaca
     6301 aaggagtgtc aatgcttcca gggacttatt tttttgacaa tctagtggat gatagattct
     6361 ttaggataaa tatagctcgt ccaagcatgg aagaactaga aaagggaata tctataataa
     6421 gtgataatat agatgagttt ttttgtgaat ataaaaacaa gttagatata aaaagcaata
     6481 aattatttta ttaaataaat agaggttttt taggaggaaa aaatgtttga caagaagaaa
     6541 ttagatagga ttaatgaatt agcaaagaaa aataaagagg gaatcctttc tgctgatgaa
     6601 atcaaagaaa gagaaatttt aaggaaagaa tatttagaaa actttagagc ccactttaga
     6661 tcaagactag atagtgtcaa agtagtttca ccagaagaat atgagcaata catgaaaaac
     6721 aataaaaatt agaacttagt aggaggaaaa tatgaactta aagtacgaat ttaagaatgc
     6781 ttgggatttt gaaagaaaag aaaatactat tgaacaaatt atgcaatatt ctcgcaatta
     6841 tatggagttt ttaagtaagt caaagactga aagactttca gtaaaagaaa ttataaaatt
     6901 ggctaaagaa aataactata tttcaataga tgaagctatg gaaaaaggaa gcataagttg
     6961 tggtgataag atttatgtca taaataaaga gaaggcagta gcactttttg taataggtaa
     7021 aaattatata gaaaaaggta tgaagattat aggaagccat atagattcac caagattaga
     7081 tttaaaacca aatccactgt atcaagagag taatctaggt ttttttaaga ctcattatta
     7141 tggtggaata aaaaaatacc aatggacggc tattccactt gctttacatg gtatagttat
     7201 attaaatgat ggaacaaaag tggacatatc tattggggaa gaagatagtg acccagtatt
     7261 ttgtgtaact gatttattga ttcatttggc tggagaccaa atgcaaaaga aactatcaga
     7321 aggaattagt ggagaagctt taaatatatt aattgggaat attcctttag aagatgaaga
     7381 aaaagaacct ataacagcaa atatattaaa gattttaaat gagaaataca atatagttga
     7441 agaagattta ttaagtgctg aaattgaagt agttccagct ggtaaggcta gagatttagg
     7501 gcttgatagg tctatggtgt tgggatatgg gcatgatgat agggtatgtt catatgctgc
     7561 tgtaaaagct atactagaaa ctgaacaacc agaatttact tcagttgcat tatgtgtaga
     7621 taaagaagaa attggttcta aagggaatac aggtatgcac tcaaagtttt ttgaaaatac
     7681 tgtggctgaa ctgattgctt tagaaggaga ttattgtgat attaaagtaa gaagagcttt
     7741 agcaaatagt aaggtattat cagctgatgt ttctgctgga tatgacccaa attttggaga
     7801 agcatatgaa aagagaaatt cggcatatat gggaaatgga gtagttttaa ctaagtatac
     7861 aggttcaaga ggaaagagtg gatgtaatga tgctaatgct gaatttatgt cagaagtaaa
     7921 gggaattttt aataaaggaa atgtaatatg gcaaactgct gaacttggaa aagtagacca
     7981 aggaggaggc ggaactatag cccatatatt ggcaaatcaa ggtgcagagg ttatagactg
     8041 tggtgttgga gttttaaata tgcatgcacc acatgagatt gtctctaaag tagacatata
     8101 tgaaatgtat aagggatata aggcattttt taatataaat ttataagtaa aaatataaat
     8161 gtaataagtg aataaaggct gttgtataaa caatttaatc tgttgtacaa cagtcttatt
     8221 tttatgtaaa aatagcaatt tgtcacacta gagaaaataa aagaaaatac tgtcaaatta
     8281 aaaattattt aaaaaaaaat tgacaacgat ttcatcttgt tataatatgt aataaagaac
     8341 ttgttatata atgttataaa aatgttacat agggaggtga gcaaaatgga tgaaaagaga
     8401 caaaaaaaag aaatggattt agaagtaaat tcctttgttc ctttatatca acaactatat
     8461 gacaacataa aaaaacaaat agcatctggc atatataaac caggagataa acttccatct
     8521 gaaggagact tatgtaaaga atttaatata agtcgtataa ctgtgagaaa tgcacttaat
     8581 gaacttgtaa aagaagatat attatgtaaa aaacgtggta aagggactta tgtaacaata
     8641 ccagagagaa tagaagcaac atgtgcagga aatagtttta ctaattcttg tcatcgtatt
     8701 aatgcaaaac caagtaccaa aataatctct gttttaataa aaaaagcaga taagcaagta
     8761 gctgaagctt taagtattga actagaagag aaggtaatat gtattaaaag acttagatta
     8821 atagatgaag tacctgttat ttttgaagtt gactatttta gaatagatta catgttttta
     8881 ttaaaagaag aattagaaga taaatcttta atggaggtta tatctaataa tataagcaca
     8941 ttacctaaac gagtagaaaa tatatttgaa gtaaaacatt caaacaaaga atattctgac
     9001 catcttaaat gtgcaagtaa tatgccacta ttgaaagtaa gacaatcggt gtatacagaa
     9061 aataatgatg tcttatatta caatgagcaa tttataagaa gtgataagta caaatatgca
     9121 gtatcagcag aaatataatc tataatttag taggaggatg agtatgaaaa agataatatt
     9181 agcatctcat ggagactttt caaaagggct tttaaatgca gtaaaaatga tagtaggaga
     9241 tttagcagat tgtgtcagtt cgtatggttt gtaccctgga caaagtgctt cagattttgc
     9301 tttagaatta gaaaaaacaa tatcagaaga taaagaatgt gaatatataa ttttatctga
     9361 cttatatgga gcaagtgtat gtacagcaat gttaagatta actaatctac ataatgttag
     9421 gttattttct ggaatgaatt tgaatatggt attagaactt cttacaaggt tttctgatga
     9481 attaacagtt gaagatatag accaattagt ggaagaatct agaagaggga tacaaagcgt
     9541 gacattacaa attaaagaag aagaagaggt attttagttt atgaaaaaaa agggaatatt
     9601 atcagtattt aaaagtaaaa aaccaattat agcaatgata cacttaaaag gagatactcc
     9661 agaagatatc tttgaaagag ctaaaaaaga gattacaatc tttgaagaaa atggtgtaga
     9721 tggaataatg ttagaaaatt actatggtaa ttattatgat ttagaaagaa tacttgaata
     9781 tgtatcaaaa gctaatttat caataccata tggagtaaac tgtttaaatg ttgatactat
     9841 gggatttgaa ctggcaacaa aatataatgc tagttatata caggttgatt ctgtagtagg
     9901 tcatgttaaa catagagatg aagctacttt agaagagttt tttaaattac aacgttcaaa
     9961 atgcccagcg tatttaattg gaggcgtacg ttttaaatat cagccagtat tatcagaaaa
    10021 tgatgttgaa gaagatttaa aaataggaat gacaagatgt gatgctatag cagttacaga
    10081 aaatgctaca ggtcaagaaa catcaatgga aaaaatagaa ctgtttcgta aaaatttagg
    10141 agacttccca ttggttatag ctgctggagt tacattggaa aatgcaaaaa aacagcttaa
    10201 attaggagat atggcaataa taggaagtta ctttaaagat aattacaaag attttgggga
    10261 tgttagtgta gaacacgtaa aaacattcat ggatgaaatc aaaaaaatta gggaggaatt
    10321 ataatgatta aattagtaag agtagaccac agacttatac atggacaagt agcatttaca
    10381 tggacaaagt ttttaagtac agactgtata ttgatagcaa gtgatgactt attaaaagat
    10441 gaattaagaa tggcaggact taaaatggct aaaccatcta atgttaagtt agtaatgaaa
    10501 agcatagcag attctataaa agcacttaac tcaggtgtta ctgataaata caacttattg
    10561 atactttgtg aatctgtaga agatgtttac agacttgcta aagaggtaaa agctattaaa
    10621 tctataaacc ttggtggaac aaaatcagat gataatcgtg aaaatatatc taaagcagtg
    10681 catgtatcaa aagatgatat aaagatgatt aaagagttag attcagaagg cgtaaatgta
    10741 tttgtacaat tagttcctga tgatgatgct acaaatgtaa tgaaattaat ataattttag
    10801 ggggatgaaa atggaattta cacaagttat tttgataact ctaattgcat tctttgcata
    10861 tatgcatagt tttgtaggtt ctacaatgca taatagacct atagttgttg caccacttgt
    10921 tggtttagca ctaggtaatc ttcatacagg tatagtaata ggttcaacat tagaattagt
    10981 atttatgggt gcatttcctg ttggggcaag taatcctcct gattttgtgt ctggttctat
    11041 tattgccaca gcgtttgtaa tattaacggg tcaagatgta tcagcagcag ttgtacttgc
    11101 agttcctata gcaactctag tattgcttat agataacttc ttaatgacag tagtacttac
    11161 atggggagct catattgctg accgttatgc agaagagggg aatattgaag gcgtagaacg
    11221 tgtgcaatta ttatttggta taggaaataa attaatatta gcaataatag taggtatagg
    11281 attttcatta ggtgttccag taatagaaaa aatactttca tttataccat catatgtaac
    11341 tcatggaatg gatgttgcag caggcgtgat tccagcaata ggttttgcca tgttagctag
    11401 aatgatgctt aataagaaaa cagtagcatt tttattatta ggatttatat tggttgctta
    11461 cctaaacata acagtaacag gagttgcatt gtttggatta gcaatagctt taatatatgt
    11521 taactttgta ggagagaagg aggtaatagt agatgacaac gagttctaag aagttagaga
    11581 caatttcacc agatagtaaa ataacgagga aagacttttg gaaatgtttt agaaggtcat
    11641 tgacattaga ttcatcttgg aactacgaac gtatgcaaaa tatagcatat gcatacatga
    11701 tggcaccaat aatccgtaga ttatataaag atgataaaga aaagaaatct aaggctttaa
    11761 aaagacattt agaatttatg tcagttactc ctcatataag tactcttttg gtaggtatat
    11821 ctggagctat ggaagaagaa aatgcaaaga ataaagaatt tgatgctaat agtataaatg
    11881 ctgttaaatc aagtttaatg ggtccagttt ctgggatagg agattcattt ttctggggaa
    11941 cattaaagct tatagcagct ggggttggta tagctttagc ttctcagggt aacataatgg
    12001 gaccgatttt attcctttta ataataaatg ttcctcattt tattatacgc tatatatgtc
    12061 tagataaagg atttaagtat ggaactcaat tctttaaaga tgtgagtggt tcaagtatag
    12121 tttcaaaagt tatggaagca gcttcaatgt tagggcttat ggtaattgga ggtatgactg
    12181 catctaatgt aatgttaaaa cttagtgtta atgtaggtag tggagaatgg gcagagccta
    12241 ttcaaactta cttagaccaa ataatgccat gtatgcttcc agctatgata ttcggtatta
    12301 tgtattggtt attaggaaaa aaggtaaaga caactacgat attaatttca gttatgatta
    12361 tttgtatagt attggctgct attggagtag tataaataaa aaaatcccaa gttcatttga
    12421 acgagggatt ttttgactaa attaaataaa aaagaaagat ggttcataat aatttataag
    12481 catttatgtg atagttataa ttagcttatt aattagttat aaactcaaaa ttaagcacta
    12541 ctttattaaa gttattatat tataatttcg aaatactcac ataatctgtt tagaaattta
    12601 tcttctaatg gggaaaaagt tttcttttta gagtaaatga agaaaaactt tctagtaaaa
    12661 tcaacatcct ttattttata acattttatt ttacctgcat tgagataatc aatagctgaa
    12721 atgtacgata taaaggatac tccaagacct aatctaacca tttccttaat agcctcattt
    12781 gattctacat gagcgattac atttaattta cttactggaa agttattttt actaagtgtg
    12841 tttagtatta agtttcttgt acctgaccct tcttttctca taataaaatt tagataagcc
    12901 aattctccta tatcgataca attattttta ttatcaattt taaaatctga aggagtgata
    12961 agaacaagtt catcatctaa taagtctagg tactctattt gaggattatt gattttagaa
    13021 ccaactaatc caaagcttat tcgttcattt aaaatttctg atatagcata ttgagaatca
    13081 taatggctta tggaaaattt tacatctgga taactcatag aaaaactctt cataaaatca
    13141 ggtaaaatat aagtttcagg tatggaacta catgctatat ctatgatacc ttctattttt
    13201 cctgaatatt cttttatatc ataaattgca cgtttgcaat tattcaatat atatatagca
    13261 tgttcataaa gaatttcacc agattttgtg agtgtaataa ctttgttgct tctatttata
    13321 agaacagttt ctagctctct ctctaaattc tgtatgtgtg cgcttacagt aggttgagtt
    13381 aaaaaaagtt ctcttgcagc ctttgaaaaa ctctgatgtt ttgcaacagc aacaaatact
    13441 tcaagttgtt taaaatccat tatttaatcc tccttaaatt atctaaaact attataacat
    13501 atttgataaa ttaatagtca aaggtgattt ttaaaataga aaacctttaa atattcatgg
    13561 agtttttgat tgtttaggaa taatttggta taatgttgta aggtacttac aataaaaata
    13621 tcgtaaattg aattttcaaa attcaacttt aaagtgtttt ttattacaga aaaattacaa
    13681 ttagcttctt aatcttatta aattcaaata aatattatat aattatttta taatataata
    13741 aatataatta ttgaaaaaag agataactgt aaattgagat atagataaat actataaaat
    13801 ttaagaaagg aggattgatt tatgaagtta aaaaggtcat tagtttgtgt aactatttta
    13861 ggtattatac ttgttggatg tcataaagaa aatactaaag agaaaaatca agtagcaagt
    13921 aaagcaacac agcaaaaaac tatgacaaaa gttcaaaatg atgttaatga aattatgaat
    13981 aaagattata agtacattat aaagaatatg ggtatacctt ataatacatt ttattatata
    14041 aaacctaaag ttttaaaaga atcaaataca atgcaagata taaatacatc aagttatatg
    14101 acattagttt atccaaaata tactggaaat gatgaactag atggaagtgc tttatatgtt
    14161 gatattaacg aaaataaagt tgtcaatgtt gaaaccaatt ctttttcatc acaaggtata
    14221 tcagtcattg atgctgaatc tagtatagtg attgaaaaaa gtgaccatga aaaatcggct
    14281 gtatcactag aaaattttag acatattgac ctgggagagt atgttggtgt tgaagattcc
    14341 agaataaatg aaattgtagg agatgcgaat tatgatttaa cagcatataa tcatgaaggc
    14401 agtaaagtag ttaagagtta tagattgaaa gaagataaca aaatattaaa aaaagaggta
    14461 cttacaataa gtattgtgga taacaaaata aaatctataa aaactattga aagtgataaa
    14521 attgtaaaaa taataaaagg aactttatta gaataaaata ttctaaagca tatgatgctg
    14581 tttcaagata gaaggaaatt tctagctggg aacagttttt ttattagatt tataaaattt
    14641 gaactgcact tatagataac tattagttat aattaaatat aagataaaag tatttccaac
    14701 ttttataatc tataatatat tacatagaaa tatcttaaat aaaaaatata ttggggagta
    14761 tttaaaatga tagaattaga agatgccata aaaattattg aaaaaaatgt tttaccaata
    14821 aacagggtca agatagtaaa tcttatagat gcagtaaata aggtagttgc agaagatata
    14881 tattcaacaa ttgatagtcc tccatttgat aggtcaccct atgatggtta tgcatataat
    14941 gcaaattctg aaaatagaaa tttaaaggtt atagggactt tgtatgcagg agatgtattt
    15001 aatagggtat taaataaaaa tgaagcagtg aagataatga ctggtggaaa gataccacag
    15061 ggtgcaaatt gtgtaataaa aaaagaagat gtaacagtta taggtgatat gataacttta
    15121 aatataaaat taaaggaata cgataattac atatttaagg gtgaggatat aaaaaagggg
    15181 caattgatta ttaataaaaa tcagaatata ggatatgatg gtattggagt attagcaagt
    15241 attggaatat ccaaagtaac tgtttatgat gatttaaaag ttggaatttt gaatacaggt
    15301 acagaactac aagatataaa tgagacttta gagaatggta aaatatacga tagtaataga
    15361 tataccttat attcaagatt attaaaactt ggaatacaag cagatacaat atgcaattgt
    15421 tctgatgata tattggagtt agaaaaaaag gtaaagaata tgttacagag tgtagatttt
    15481 ttaattacca caggtggtgt ttctgttgga gataaagatt taattccaga tgtattcaaa
    15541 aaaatagggg caacagaatt attttggaag ataaacatta aacctggtgg agcttgttat
    15601 gtaggtacat tgggagaaaa gctattattt ggtctttctg gtaatccaca tgcagcaata
    15661 gtggtatttg ataatgtgat agttcctgtt attgagcatt taacatataa gaagggaaca
    15721 catcatataa aagctatatt taaaggagaa tttaataaac tttcaaataa agatagattt
    15781 gtaagtggaa aactatatac aagtgaaggt aaattgtatg tagagctaaa tgataagaaa
    15841 gatgcaaaag ctagattatc tgcaactcta agaagtaatt gtatgattaa gattaaaaag
    15901 aatgagacaa taaaagaaaa tcaattagtg gatgttgtta ttagatagtt ttatgaaagg
    15961 acacaatttt atgtataata tattgtcagt tgtctcatat tcaggagtag gtaaaactac
    16021 gttgattgaa ggcataataa aagaattaaa taaaaaaggg tatcgtgttg gagttattaa
    16081 acatacctgt catgattttg atatggatga ggaaggaaaa gatacttata aacatagaaa
    16141 atctggggcg agaaaagtct gtataatatc agataaacga gtagcatata ttgaagaact
    16201 aaaagagaag aatccattat ataaaatgat tcaattatat gaagatatgg acttgattat
    16261 catagaagga tataaaaatt atagatttaa aagattggaa gtaactagaa aaggtaagta
    16321 tgaggatatt ttatctaata agtctgattt gataggtatt gtcagtgata tagaatataa
    16381 attgaatatt gaacagttta atttaaatga ttataaaaat atatcaaagt acatagagtc
    16441 ttgtataaaa aatgaaattt taagtataag caatgttgaa ataaaaaaca tattaaaaga
    16501 ataattttaa gatgttcttc ttatataaaa tgatataatt atttatataa ataaagatat
    16561 aagaaattta tcaattttat tttgagttat aaaatattaa agaaattgta ggaggacaat
    16621 atatgaaaaa aatattagta gcctgtggag caggaatcgc aacatcaaca atagtttgtg
    16681 acagagtaga aagattggtt aaagaaaata atatacaagc agaagttgag caatgtaaaa
    16741 ttgctgaatg ttcaacaaaa caagaaggtg ctgatttaat cgtatctact actatactac
    16801 caacaactta tgatatacct acaataaagg caacaggata tataacaggt ataaatacaa
    16861 cagcattaga taaaaagata ttaaatgcat tgaagtagtt ttgtatttat aaaaaaaatt
    16921 tagtattaaa tatagagttg tatctttata aaattttaaa tatagagata tagctctttt
    16981 tatgttgttt caagtataca actatatgat ataaatcttc aattttatga aaaagattgt
    17041 tattactttg taagtttata aaagatttaa agttattgtt atgttatact aatttatggg
    17101 tattttttag aagtactaat aaaggaggga tttaatactt atgataaaac aatttgtcaa
    17161 atattataag ccatataaga aaatttttac actagacctt atagcagctt ttctattttc
    17221 cttatgtgat ttagtttatc ctatgataac tagaaatata atggatgacg ttgttccaaa
    17281 taaaaactta agaatgttag tagtttttgc agtagcactt atacttatat ttatagcaaa
    17341 agcagggctt aactatttta tgcagtattg gggacatgtt attggtgtag atatgcaagc
    17401 tgatatgaga aatgaggtat ttacacactt acaaagactt ccaaatacgt attttgataa
    17461 taacaaatct ggagtaacta tgtctagaat agtaaatgat ttaatggata taacagaact
    17521 tgcacatcat gggcctgaag atttattcat ttctatagta atgttggttg gttcattctt
    17581 tatacttata gatataaata ttcctcttac gcttataata tttgctatat taccgtttat
    17641 aatttggttt gcaatagcta aaaaagataa gatgaatatt gcttttatga agagtagagt
    17701 tactataggt gatgtaaatg ctactttaga aaatagtata gcaggtatga aggtaactaa
    17761 gtctttttgt actgagaaag aagagttaaa taagtttgta agaagcaata aattatttag
    17821 aagagcaaga caagattctt ataaggtaat ggcagaatat tactctggta tgaatttata
    17881 catggatatc ttagagtggg tagttgtaat agctggtggt tattttactt atataggaaa
    17941 gattactttg ggtgattttg ctgcttatat actatatgta aaaatgttta tacaacctat
    18001 gaaaaaatta ataaacttta cagagcaata tcaaaatggt atgacaggtt ttaaaagatt
    18061 catagagata atggaacaag accatcaaaa agaagctaaa aatcctattg aacttgaaaa
    18121 tgtaaaaggt gacattgaga tagaaaatat atctttcaca tatgaagata agactcaagt
    18181 tttggataat ttaagtttat ctataaaagc tggtaagact atagcattag ttggaccttc
    18241 tggaggagga aagactacat tatgtaatct attgccaaga ttttatgaat ttgataaggg
    18301 tgatataaaa attgatggaa agagtataaa agatgttagt ttaaaatcat tgagaaaaaa
    18361 tataggtata gttcaacaag atgtattttt atttacagga actataagag ataatattct
    18421 ttgtgggaac cctaatgcta cagatgaaga aatgatagct gctgctaaaa aagctagaat
    18481 acatgacttt gtagagactt tgccagatgg atatgatact tatataggag aaagaggcgt
    18541 aaaattatca ggtgggcaaa aacaaagaat ttcaatttca agaatctttt taaagaatcc
    18601 tccaataata atattggatg aggctacatc tgctttagac aatgtaacag aaagagaaat
    18661 tcaagagtct ttagaggaat taagtaaaga tagaacaaat ttagttgttg cacatagatt
    18721 gactactata aaaaatgctg atgaaatcat agtattgacg gataaaggaa ttgaagaaag
    18781 aggtactcat gaagagctag taaacaaaaa tggagtatat agtaggttac acaataacta
    18841 gtatacaaat tttacaccat agctaacata cacattaaac ttacaaacat aggatatatt
    18901 aagattcata aagacaattc attttatatt tataagatgt tttatgaata aaatgtatcc
    18961 tatgttttat tttttgcaaa ttacatttaa ttctctttgt aagaatagag ttaaatagcc
    19021 aatggtcggg ataacgtttt tacaaaaaga ataatttaga aaactaactt taatttatgg
    19081 aatttatcga attttatgaa aaaaatcgaa ttttgacttg aaatgttttt caaaagtagt
    19141 aaaataatgg ataactatac atacatttta taaaaatata tttattatga ggggggattt
    19201 tgtatggagg caataacagc tttttgtcta gttttagtat cactgacgat aggagatata
    19261 gtttcagcaa aaactaaagc ttttgttccg tcggtttttg tatcagcaat tatatttata
    19321 gtaggatttt ggactttttt cccagaaaat attgtggact tagccgcttt aggaacacca
    19381 ttagctcaat taggtatgct tttgttaata actcatatgg gaactatgat gagtattaag
    19441 gaattagcag gtcaatggaa aacaatagta atagccttgg caggaatagt aggaatatgt
    19501 gtaggtgctt tggcattagg gacagtagta tttggatggg atacagcagt aatagctaca
    19561 cctcctctta ctggagggct agttgcatct ataatgatgg cagatgctgc tacagcaaaa
    19621 ggtcttgcaa gtttatcagt acttgcaatt ttgatgtatg ttgcacaagg atttgcgggt
    19681 tatcctatta cagcattaat gcttaaaaaa gaaggaaaaa gacttttatc agactttaga
    19741 agtggaaaag taactgtaaa tacagatgaa gaaaaagtta aggatttacc tgaacaaaaa
    19801 agcagattta agataatacc agacttacca gaaaaatatg atacgacata tatgataata
    19861 cttagattag gatttgtagc atggttagca gtacaattta caaatcttac aaatgaagtt
    19921 gtatcaagat atgttatatg tttaatattt ggtgtaatag catcagaact tggaatagtt
    19981 gatagaaaac cacttaataa atctggttca tttggatggt taatgacatc attgatggct
    20041 tttatatttg cagggttagc aaaagctaca ccagcaatgc ttgttgaaat ggcagttcct
    20101 cttgtaggaa tcataatatt tggtgtaata ggtatgggaa tattctctat tatagtaggt
    20161 aagcttttag gtgaaagtaa agaaatggct ttttcagttt cgcttacatc actttatggt
    20221 tttccaccaa actatatatt aactgaagag gcagtaaagg ctttggctga aacaccagaa
    20281 gaaaaagaat ttttaatgga taagatgctt ccaaagatgc ttgttggagg atttactaca
    20341 gttacaatag cttctgtagt aatagcaggt atctttgtaa atctaatata gtatttgagt
    20401 gataaaaagt attttaaata agaagaatgt gtactacttg gaggtatgga atggatatta
    20461 aagaaataac gaagagttat aaagactatg taattaagct tagaagggaa tttcatgaaa
    20521 atcctgagaa aagtatggaa gaagttagaa catcaaagag agttaaagaa gaacttgata
    20581 aaatagggat accatatgta tcagctggag gtacaggtgt aattgcaact ataaaaggag
    20641 ctaatccagg aaagactgta gcacttaggg gtgatatgga tgctctacaa gttgttgaat
    20701 gcactgatgt agaatacaaa tctaaaaatg aaggacttat gcatgcttgt ggtcatgatg
    20761 gtcacacatc aatgcttcta ggggcagcaa aagtgttaaa tgatattaag gatagtatca
    20821 atggaactgt aaaattattt ttccagccag gagaagaagt tggtaaaggt gcaagagcta
    20881 tgattcaaga tggtgctatg gaaggtgtag atagtgtgtt tggaatacat ctttggactg
    20941 atgtagaatc tgggaccata tcggtagaag aaggaccaag aatggcttct gctgatttct
    21001 ttaaaattac tgtaaaaggt agaggtggtc atggttcact tcctcatcaa ggtgttgatg
    21061 cagtgcttgc aagttcagca atagttatga atctacaatc tatggttagt agagaagtaa
    21121 gtcctctgga accattagtt gtgagtgttg gagttttaaa ttctggaact agatttaatg
    21181 taattgcgag tgaagctatc ttagaaggta caataaggct atttaatcca gagcttagaa
    21241 agcaaattcc tggaatttta gaacgtatag caaaatctac agcagaagca tatagagcag
    21301 atgctgaatt agagtatggg tacttaacac cagcagttat aaatgacaaa gaatgttcaa
    21361 agatagctac agatgcagca attaagctat ttggagaaga ctgtataaca ctatttgaaa
    21421 aagtaacagg agcagaagat ttagctgagt ttatgaatat agcacctggt gcactagcat
    21481 ttgtaggagc tagaaatgag tcaaaaggtg cttgttatcc tcatcatcat ggatgcttta
    21541 atatagatga agatgttcta gaaattggaa ctgctttata cgtgcaatat gcagtggatt
    21601 tcttaaataa ataattttaa tatactataa agaaaaagca gagactaagt ttatatctct
    21661 gcttttttat tgtgtatatt ttttataaat attgactata taaaatatat atgatagcat
    21721 aaagaaaatg ataatcaata tcaattaaaa agaaggtgat ttatttgaat aatgtaatat
    21781 ttgcattttt gataacatta ttggctggtt tatctactgg tataggaagt tgtatagcat
    21841 tttttgctaa aaaaacaaat actaagtttc tttctataag tttaggattt tctgctggtg
    21901 ttatgattta tgtttctatg atagaaattt ttcctaaagc acaagatgct ttgacaaaat
    21961 ctatgggaga gaaattagga agttggtcaa ctgtaatagc tttttttata ggtatggcta
    22021 tcattgctgt tattgataag ctaattccac aagaagaaaa tccacatgaa ataaagaaaa
    22081 tggagaatat agaagaaaag aatatcaaaa aaaataagtc tcttttgaga acaggtatat
    22141 ttacagcaat ggctatagca attcacaatt ttcctgaagg attagctaca tttatatcag
    22201 ctcttgatga tgttacaata gctattccga tagcaattgc aattgcaata cacaatatac
    22261 cagaaggtat atctgtatct gtaccagttt attatgccac aggagataaa aagaaagctt
    22321 tttattattc ctttttatct ggtatgtctg agcctttagg agcgattatt ggatatgtac
    22381 ttcttagaaa ttttttaaat gatataactc ttggaattgt atttgcaatt gtaggaggaa
    22441 ttatggtatt catatcacta gatgaattat tgccatcagc tagagagtac ggggaacatc
    22501 atttatctat ttatggattg attgcaggaa tgggtgttat ggctattagt ttattactat
    22561 ttaaatagct ttttaaatat atgattgtaa cctaaaatta gtatcaaaat gatataatta
    22621 tttaaataga tttttaagct atttaaaaat atcattttgg agaatgagtg ctatggaaaa
    22681 gaaaataaaa ttcaaatttg actggtatag ttcacctgat aagatagaga aatggttaga
    22741 agatattgaa gctaaaggat ataatttata taagataaat aaatttggag gaatatttta
    22801 ttttcataag ggaagtccaa gaaaaataaa atacactgtt gattactact atgatgtaga
    22861 gaataattat tttgatgaac ataaatcaaa tggctggaat cttgtattta ggagtattgg
    22921 tagttttatg ggtacatctg catggtatat atggtcgaaa gaatacaaag acatacctcc
    22981 taaattatat agtgattctg ataaaacaaa taaagtaaag tcaattttat ttatgaatat
    23041 gtttatattc tttatgttag tagtaatatt tatatattta ttgaatattg aaataagaaa
    23101 tttatatagt ggttatggag catctgtaac tgctattgta ctacagatta tattgttgat
    23161 tttatatgca gtacaattat ataggagatt aatgtattat ttaaggctta aaaagtttaa
    23221 ataaataaaa gaaggtgtcc taaaattatt tttttaatat tttaagacat cttcttttgt
    23281 cagtaagtac tgtttttaat tatagtaatt tctgtcttta aattgaaata tcaaataata
    23341 gttgtgtggg attgagtatt gataaaaata atttaaaaat gttatcctaa agtatcagat
    23401 atacaaaatt atctttaaat atattataaa tattttaatt gtattaacta gaaattatgt
    23461 aaggaggcat tttatgatta agatagcagt ttgtgaagat gaaaaagaaa cacaactttt
    23521 aatagaagat taccttgaga atatattaaa agatataagt atagaatatg agatacaaaa
    23581 gtatatatct ggagaagagt tactggaaag taatttaaaa gatatagata tattacttct
    23641 tgatataaaa atggaaaaac taaatggaat ggatactgca agaaagatta gagaagtaga
    23701 caatgaaatg gaaataatat ttgtaacttc attaatagat tatgtgcaag aaggttatga
    23761 ggtaagagct tataggtatt tactaaagcc aatagagttg gaagaactaa aaaaacatgt
    23821 gctaacttgt atcaaagata ttgaaataaa taaggaaagt catattacaa taaaaaataa
    23881 gtctaataca tataagattt atttaaatga aataaaatat atagaagttc aaaaaaaaga
    23941 tatgctaata cacacaataa acaagaattt tgatataaaa tatagtttag gcaaaataga
    24001 aaaagaatta aatccatata aatttataag atgccataaa agttttatag taaatttaag
    24061 atatgttgaa aatataaagc ctaatactgc aatactggaa agtggagaag aggtacctat
    24121 tagtagatat agatataaag aagttaagga gaaattttta aaatttcttg gtgatacaat
    24181 atgctaaact ttatgactga gctattagta gttttatcta tatttataca gttttctata
    24241 atgaacggag ttttaattta taaaaaggat agtaaaaaaa atgaaaaaat agtattctat
    24301 attgtcttgt ttcttattta tattataatt cagataagta gattatttaa tggaggaaat
    24361 tactttagta atataaacta tttattttat atattagcat ttattgcttg gggaggatac
    24421 tataa
//
LOCUS       DAKBNN010000041        23739 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000041 DAKBNN010000000
VERSION     DAKBNN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.041"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1575
                     /locus_tag="OUB02_001417"
     CDS             <1..1575
                     /locus_tag="OUB02_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885183.1"
                     /translation="IYIVLEMIDLMAVEIYNLYSTTFMKRFTRHIEILMLEKANRLTT
                     KDFENKEIYDLINRAQSQNGATVIDYFNSWILIFQQAITIFSSIIIIISYQYWIVILI
                     IIAPVIQFYYSQKLGHEQYEISMKRTSEERKCWYINFLMLLGNSIKETILYGLGNLFI
                     NQYGKLKEKFILQDYKIMKKTFEMNFIIIIIGQLFTGVIYFHILLNGFWQKIYMGDVT
                     TYITCITTIKNGIEVILSMMSTIYSKSLYMELLFRFFDIPEKTELNKTKLKIDNIEKI
                     ELKHLYYKFTEDSGYVLKDINLELKKNSPIALVGRNGSGKSTLIKIILGIYDDYEGDI
                     LINGINLHNIEKENYYNKISCVFQDYTKYEASVKENVGYGNLDQINNEDLIKESLIKS
                     GLDINQLGSNKLNTILGYWFGENQLSEGQWQKIAISRAMMKDADFYILDEPDAALDGI
                     AENDMLLTYKKMFENKFGIFVSHKTHHLNLLCENIIVLNNGQIAECGNHEQLIEKEGI
                     YHQLYSSQIISKLETN"
     gene            1604..3031
                     /gene="ccpM"
                     /locus_tag="OUB02_001418"
     CDS             1604..3031
                     /gene="ccpM"
                     /locus_tag="OUB02_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HCT8885184.1"
                     /translation="MEQKPFIHLFQTSTGFYFYDVNTDSILNIDENVYDYLKKEQLGE
                     SSSNTLEYVEFLRKQGYLKSNKVKITEHPETEVLKYHCENRVEGIILQVTQNCNLHCD
                     YCTYSGGYINRIHTNKRMSKETAKRGIDYLISHSRDCQYVSIGFYGGEPLLEFDLIKW
                     CIEYSKANIEGKNLKYNLTTNATLITEEIIELFEENNVSIMISLDGPAEIHDKNRKFA
                     NNNVGSFSTIMKKLDMISTKYPNYFKNNVHFNSVLATDNFSCVNKFFTKEELFQNSLF
                     LSSIVSEVNAKVKTEKSQSFYEESQYALFIGYLTLLGRIKKEDSSKLLQTQISTIGDT
                     RNGKQGKQRLVMPEKWHHGGPCVPGVMRLFINVDGYFYPCEKASEASDSMNIGNLDQG
                     YDLKKVEKLLNIERINGDKCHSCWAYENCRICAVSLSVDSTQEDIAKECEYIRSSIES
                     DFQDYCVLKELGFDFETKELIENTI"
     gene            3094..4170
                     /locus_tag="OUB02_001419"
     CDS             3094..4170
                     /locus_tag="OUB02_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HCT8885185.1"
                     /translation="MKKVLIYPFTEEMAPFVKQENLLKDIDITSIVSLKGNGILGDKY
                     KFNGKSLEVSENFDKELEKCTYILLNNFYSDESENIILENIKKAVNHGKKIIFFSNSN
                     SNIDNLKMIIPKEQQFEINSSKNMVKDFQGRIYNIDTPVIFVCSIFEGLEKFDIQLAL
                     RNKLLIDGYKVLQIGTRQVCELFDFYSFPEFMYSNNIEEQDKVLMLNNTLKKLELEKK
                     PDLIIIGIPGGILPFSSKAFGDFGITMYKVLQAVTPDCTILSIPYGKYSDEEISYFGK
                     ITKSKFNIEVDYYNIAAKSILSHPTETMGKPFYLTLGKSLVEKKIATLQNKNVFCLSN
                     KSEIDRLIELMIKEFIKYGTVASI"
     gene            4204..4494
                     /locus_tag="OUB02_001420"
     CDS             4204..4494
                     /locus_tag="OUB02_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HCT8885186.1"
                     /translation="MVNTEVMNKLDIYTVLVNIFEKRSKINFDENCYLKNENLFESKI
                     MMPARELVLTLYDIEDYFSISIPEDVILSGRFNSFNNIYDIVKKLENSINFN"
     gene            4536..4754
                     /locus_tag="OUB02_001421"
     CDS             4536..4754
                     /locus_tag="OUB02_001421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885187.1"
                     /translation="MSNFGKRNLYDSANGEIDPKGGCNCGALCANLCSCSCSGGTWSF
                     YHTNQVKGSKINNGQQFRSPGEIPGQKK"
     gene            complement(4958..6625)
                     /locus_tag="OUB02_001422"
     CDS             complement(4958..6625)
                     /locus_tag="OUB02_001422"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCT8885188.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMNDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNSY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(6822..7544)
                     /locus_tag="OUB02_001423"
     CDS             complement(6822..7544)
                     /locus_tag="OUB02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8885189.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            7935..9362
                     /locus_tag="OUB02_001424"
     CDS             7935..9362
                     /locus_tag="OUB02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8885190.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            9355..10134
                     /locus_tag="OUB02_001425"
     CDS             9355..10134
                     /locus_tag="OUB02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HCT8885191.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            10397..11122
                     /locus_tag="OUB02_001426"
     CDS             10397..11122
                     /locus_tag="OUB02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8885192.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            11252..13192
                     /gene="mngA"
                     /locus_tag="OUB02_001427"
     CDS             11252..13192
                     /gene="mngA"
                     /locus_tag="OUB02_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCT8885193.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIHKKIRY"
     gene            13321..15936
                     /gene="mngB"
                     /locus_tag="OUB02_001428"
     CDS             13321..15936
                     /gene="mngB"
                     /locus_tag="OUB02_001428"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCT8885194.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEIGHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNFV
                     NVECLMNQVKTILVK"
     gene            16196..17350
                     /locus_tag="OUB02_001429"
     CDS             16196..17350
                     /locus_tag="OUB02_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCT8885195.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKSYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTSTTTQIFNLITSIQQSKNM"
     gene            complement(17473..19386)
                     /locus_tag="OUB02_001430"
     CDS             complement(17473..19386)
                     /locus_tag="OUB02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8885196.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDDHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDKKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            19628..19945
                     /locus_tag="OUB02_001431"
     CDS             19628..19945
                     /locus_tag="OUB02_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885197.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            19953..21200
                     /locus_tag="OUB02_001432"
     CDS             19953..21200
                     /locus_tag="OUB02_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8885198.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTIVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            21229..21582
                     /locus_tag="OUB02_001433"
     CDS             21229..21582
                     /locus_tag="OUB02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT8885199.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEADGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            21604..23049
                     /locus_tag="OUB02_001434"
     CDS             21604..23049
                     /locus_tag="OUB02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT8885200.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVIEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            23441..>23739
                     /locus_tag="OUB02_001435"
     CDS             23441..>23739
                     /locus_tag="OUB02_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8885201.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQIS"
BASE COUNT         9213 a         2659 c         3977 g         7890 t
ORIGIN      
        1 atctatatag tcttagagat gattgattta atggctgtag aaatttataa tttatactct
       61 acgacattta tgaaaaggtt taccagacat atagagattt taatgttaga aaaagctaat
      121 agattaacta cgaaggattt tgaaaataag gaaatttatg atttaatcaa tagagctcaa
      181 tctcaaaatg gagctacagt tattgattac tttaactcct ggattttaat ttttcaacag
      241 gcaatcacaa tattttcctc gattattata attatatctt atcaatattg gattgtaata
      301 ttaatcataa tagccccagt tatacaattt tattactctc aaaaattagg gcatgagcag
      361 tatgaaataa gtatgaaaag aacttctgaa gaaagaaaat gttggtatat taatttttta
      421 atgctattag gaaattcaat caaagaaaca attttatatg gattaggaaa tttatttata
      481 aatcagtatg gaaaattaaa agaaaaattc attcttcaag attataaaat aatgaaaaaa
      541 acatttgaga tgaattttat aattataatt attggtcaat tatttacagg agttatatat
      601 tttcatatac ttcttaatgg gttttggcag aaaatatata tgggagatgt aactacttac
      661 ataacctgta taacaacaat aaaaaatggt atagaagtga tattatcaat gatgagtaca
      721 atatatagta aatctctata tatggaacta ttatttagat tttttgatat tcctgagaag
      781 acggaactta ataaaacaaa acttaaaata gataatatag aaaaaataga gctaaaacat
      841 ttatattata aatttactga agattcaggt tatgttctaa aggatattaa tctagaacta
      901 aaaaagaata gtcctattgc acttgttggt agaaacggtt ctggtaaatc aactttaatc
      961 aaaattatac taggaattta tgatgattat gaaggagata tcttaataaa tggaattaac
     1021 ctacataata tcgaaaaaga aaattactac aataaaataa gttgtgtttt tcaagactat
     1081 accaaatatg aagctagtgt aaaagaaaat gtaggatatg gaaacctaga tcagataaat
     1141 aatgaggatt tgataaaaga gtcattgata aaatctggac tagatattaa tcaattgggg
     1201 agtaataaat taaataccat tttaggatat tggtttggag aaaatcaact ttcagaaggc
     1261 caatggcaga aaatagcaat atcaagagct atgatgaaag atgctgattt ttacatttta
     1321 gatgaaccag atgccgcttt agatggaata gctgaaaatg atatgctttt aacttataaa
     1381 aaaatgtttg aaaataaatt tggtatattc gtatctcata aaacacatca tttaaatttg
     1441 ttatgtgaaa atataatagt tttaaataat ggacaaattg cagaatgtgg aaatcatgag
     1501 caattaatcg aaaaagaagg aatttatcat cagttatatt caagtcaaat tataagtaaa
     1561 ctagaaacaa attaataatt actttgaaaa ggagttaaga attatggaac aaaaaccttt
     1621 tattcattta tttcaaacat caactgggtt ctatttttat gatgtgaata ctgattctat
     1681 attaaatata gatgaaaatg tctatgatta tctaaagaaa gaacaacttg gagagtcaag
     1741 tagtaataca ttagaatatg tagaattttt aagaaagcaa ggatacttaa aatcaaataa
     1801 agtgaaaata acagaacatc cagaaactga agtattaaag tatcattgtg aaaatagggt
     1861 agaaggaata atattacaag taactcaaaa ttgtaactta cattgtgatt attgtactta
     1921 ttctggagga tatataaata gaatacatac aaacaaaaga atgtctaaag agactgctaa
     1981 aagaggaatt gattatttaa taagccattc aagagattgt caatatgtga gtataggttt
     2041 ttatggcgga gagccattac tagaatttga tttaattaaa tggtgtattg aatattcaaa
     2101 agcgaatata gaaggtaaaa atttgaaata caatttaaca actaatgcaa ctcttataac
     2161 agaagaaatt atagagttat ttgaagaaaa taatgttagt attatgatta gtctagatgg
     2221 tcctgctgaa atacatgata aaaatagaaa gtttgcaaat aataatgtag gatcttttag
     2281 tactataatg aaaaaactag atatgatatc tactaagtat ccaaattatt ttaaaaataa
     2341 tgtacatttt aattctgtat tagcaactga taacttctct tgcgttaata aattctttac
     2401 taaagaagaa ttatttcaaa attcattatt tttatcttca attgttagtg aagtaaatgc
     2461 taaagttaaa actgagaaat cacaaagttt ttatgaagaa agtcagtatg ctttattcat
     2521 aggatattta actttacttg gtagaataaa aaaagaggat agttctaaat tattacaaac
     2581 tcaaatctct actattggag atacgcgaaa tggtaaacag ggcaaacaac gtttagtaat
     2641 gccagaaaaa tggcatcatg ggggtccatg tgtaccagga gttatgagat tattcataaa
     2701 tgttgatggt tatttttatc catgtgaaaa agcatcagaa gcatcagata gtatgaatat
     2761 aggaaattta gatcaaggat atgaccttaa aaaagtagaa aaattattga atattgaaag
     2821 aataaatggt gataaatgtc atagttgttg ggcttatgaa aactgcagaa tttgtgctgt
     2881 ttctttaagt gttgattcaa cacaagaaga tatagcaaag gagtgtgaat atattagaag
     2941 tagtatagaa agtgatttcc aagattattg cgtattaaag gaattgggat ttgattttga
     3001 aacaaaagaa cttatagaaa ataccattta atttaagata gcatcttaaa ttattacatt
     3061 atgaaactaa ttagaatcta ttggaggatt aaaatgaaaa aagtattaat ttatccattc
     3121 acagaggaaa tggctccatt tgttaaacaa gagaatttac taaaagatat agatataaca
     3181 tcaattgtaa gtttaaaggg gaatggaatc ttaggagata aatataaatt taatggtaag
     3241 tctttagaag tttctgaaaa ttttgataag gaattagaaa aatgtacata tatcttactt
     3301 aataattttt atagtgatga aagcgaaaat attattttag aaaatataaa aaaagctgta
     3361 aatcatggta aaaaaataat cttttttagt aattcaaata gtaatattga taatttaaaa
     3421 atgattatac ctaaagaaca acagtttgaa attaatagtt caaaaaatat ggtaaaagat
     3481 tttcaaggaa gaatatacaa tattgataca cctgttattt ttgtatgtag catatttgaa
     3541 ggcttagaaa aatttgatat tcaattagca ttaagaaata agttactaat tgatggatat
     3601 aaggttcttc aaattggaac acgtcaagtt tgcgaattat ttgatttcta ctcattccct
     3661 gagtttatgt actcaaataa tatagaagaa caagacaagg tactaatgct taataatact
     3721 ctaaaaaaat tagaattaga aaaaaaacct gaccttataa ttatcggaat tccaggtggt
     3781 atacttccat tttcttcgaa agcatttggt gattttggaa ttacaatgta caaagtactt
     3841 caagcagtaa ctccagactg tacaatacta tctattccat atggtaaata tagtgatgaa
     3901 gaaatttctt attttggtaa aattacaaaa agtaagttta atatagaggt agattattac
     3961 aatattgctg cgaaatcaat attatctcac ccaacagaga ctatgggaaa accattttat
     4021 ttaacattag gaaaatcatt agtcgagaaa aaaatagcca cattacagaa taaaaatgta
     4081 ttttgtttat ctaacaaatc tgaaatagac agattaatag aattaatgat caaagaattt
     4141 ataaaatatg gtactgtagc atctatataa aatattttac ataaaggatg gtgaaatact
     4201 gatatggtaa acacagaagt tatgaataaa ctagatatct atacagtttt agtaaatata
     4261 tttgagaaaa gatcaaaaat aaattttgat gaaaattgtt atttaaaaaa tgaaaattta
     4321 tttgaatcta agataatgat gcctgctcgt gaactagtat taacattgta tgatatagaa
     4381 gactattttt ctatatctat tccagaggat gtaattttat caggaagatt taattctttt
     4441 aacaatattt atgatattgt taaaaaactt gagaattcta ttaattttaa ttaattaaaa
     4501 tataataata taacaaatga gaggggaaaa atgatatgtc taattttgga aaaagaaatt
     4561 tatatgattc tgcaaatgga gaaatagatc ctaaaggagg atgtaattgt ggtgctctgt
     4621 gtgcaaatct atgctcttgt agttgttctg ggggaacatg gtcattctat cacacaaatc
     4681 aagtgaaagg ttctaaaata aataatggtc aacaatttag atcaccagga gaaatacctg
     4741 gacaaaaaaa ataaaaagag ttaaatataa ttagttttag ctttatcaat taacataatt
     4801 aactttatta aaattaatag aatctaatta caattgttta ttatatctat aaaagcaact
     4861 ttaaaatacc ttgttttata tatgttaatt aaaaatggta ggagctgtcg tgctagaaaa
     4921 tagtgctaca gctcctacca tttttacata aataacttta tttataatat atcattatac
     4981 tttcgtaagg ttttatttcc attttatttt caattatatc tctagtgtaa ttggtttcaa
     5041 gtattgtgta gtttaattta ttcaaataag tttcaggtaa tttatatact acattatcat
     5101 gagtaaaatt acttataaca agaagatttt ggtttttata ctctctttca taaacaaata
     5161 gatttttatg attttcatct aacaatctat aagtaccttc tgtaaaaata tcatattgtt
     5221 tacgatattt gattaatctt tgataagtat aaaagactga acgagaatca ttaagctcat
     5281 tttctacact aatattgtca ttattaactt ttaaccacgg tttatgatta gaaaacccct
     5341 tataaatagt attatcccat tgcataggag tacgggcatt atctctggat ttttgttgaa
     5401 gtataagcaa tgcctccttt tcatcaatac cagatttaat catatgatag tatgaattct
     5461 tactttcagc atcatcatat tcatctattt tagtaaagta ggcatttgtc atacctattt
     5521 cttcaccttg atatatgtat gtagtacctt gtagaccaaa tagtgttatg gctaacatct
     5581 tagcactttc ttgacgaaaa aatctatcat ctccaaatcg agataaagct ctaggttggt
     5641 cgtgattaga gtaaaataga gcattccatc cctttccttc atacatttta tattgaaagt
     5701 cagattgtgc tttttttaat ttatccagag agaacatttc attagtccat tttgcgcctc
     5761 ctttataatc tgttttcata tgatggaagc taaaaaccat agatagttct tctcgatttt
     5821 ctctagtata gttgatacac tcttcaggtg tagtagatga catttcacct acagtaacta
     5881 tatttttagg tttgaaaact ttttgattca tattatgtaa atattcgtgt atacgaggac
     5941 catcagcgta gtgagttctt ccatctcctt ttggtcctgt tatagggtca tcaggtaatc
     6001 caggtgtttt ggagagtagg ttgactacat ctaaacgaaa accatcaata cctttatcag
     6061 cccaaaattc tagtattttg taacattctt cacgaacctt ttcattttcc caatttaaat
     6121 cagcttgagt ttcttcaaat aaatgtaaat aatattggtt agtggtttca tcatatttcc
     6181 aagcagttcc actaaatctg gatatccagt tattaggaac agaaccatca ggtttagcat
     6241 ctttccagaa ataataatca cgatatggat tgtctttgct ttttttagat tctttaaacc
     6301 atttatgttc agtagatgta tggtttagga ccatatccat cattattttt atgtctcttt
     6361 tatgagcttc ttttaagagt ttttcaaagt cattcattgt cccatatttt gggtctatat
     6421 tataataatc ttctatatca tatccattat ctctttgagg agaaacgtac ataggagtca
     6481 accataataa atcaacacct aaagaataca gataatctaa tttttctata ataccattta
     6541 tatcacctat accatcattg ttgctatctt tgaaactttt tggatatatt tgatagacag
     6601 ttgctttttt ccaccaattt ttcatacttg atacctcctg taatttataa gttaattata
     6661 atatgtttaa acaaattatc aagtattaaa ctgatattaa agctaaaata tacaataaat
     6721 aattatttgt ataaacatat aattgtatat agaatagtga ggttagtatg ggataaagtg
     6781 tctaaacgta aaaaaattat gtttaattaa acataatttt tttaatatct ttgagtaaca
     6841 tccatgaata caaatgtttc aggtgtatgc tttgattcag tatattcaaa aagctttcca
     6901 tcatcagtat atacactatt tttaataaca ccaacacaat tataatcatt tagaggtagc
     6961 gtttttaact caagctctgt agctttttca attttaaata tctttctgga tgaaacaatt
     7021 tttgtaccta gacactcttc tatatattta taaatagagc cttgtgctat agaaacatca
     7081 aggtctttaa ctatactaca tagaaaatag ttaatgtcga gaattttagg tatgttatca
     7141 atatatctta gtctgtgaag gtaatatact tcactaccaa ctttaaaacc tgtcttttta
     7201 gataacttat tatcaattaa gattttgctg aagtggagaa cactagtagc agtattaacc
     7261 tttttatctt ctacaatcga ttctacacct ttgaaattgt gcatatttag aaaaaaatca
     7321 ttagaacaac tattttctaa cactacaaca cctttgcctt ttatactttg aacatatcct
     7381 tctgtgttta attgagaaat tgctctacgt acagtgtttc tgctacatga aaacctttca
     7441 ataagttgaa gttcagtagg cagcatcata ttacttgtat atttaccatc aagtatttcc
     7501 tctttcaata cttcatatat ctctgaaaat ttatttttag ccatataccc ctatccctcc
     7561 ttaaaggtct ttagtaatct tattgaactg atatgaacaa attatatcaa atgtgtttag
     7621 acatgtatag tgaaaataga atcatatcaa ccataaaaag gacaaaatag aattattttg
     7681 tgggttgttt caaatacatg gttaaacata tttttaaaat aaaaaaaatg aattaaaaaa
     7741 ataggctaga tatagcaata cacttaaaag gatttatatg ttaaaccaaa tgataaaaat
     7801 aaaatgaata aaaaatattg acaatttgtt taaacaaata taatatttaa aatataaaat
     7861 gtgaataaat cgttttcata aaaaattaaa ccgaaataga aaggggattg tatatgaaga
     7921 ggttcttaaa caaaatgatg caacaactgt cacttttagg aaagactatg ctggtgccaa
     7981 tagctgtaat gccagcagca ggtatattag gattattatt tggtccaaat atgctcaatg
     8041 tgccagctat atctaatata tctaatatag tattttcaaa tgtggactac ttatttttat
     8101 taggagcagc tagtgcctac tcaaagtcta aagacaagac aagtgtaatg gttgcagccg
     8161 tagttgcata tatgatattt aagtcaaatt tagagttatt gaatgataca ttaaatgcag
     8221 gtgtatttgg tgggataatt gttggagctt gtatagcatg gacttacaat aaaacatata
     8281 aagttaaagt tccagcattt ttaagcttct ttgcaggaga aaaatgtgta attacactat
     8341 caccagtggt tgctttaatt ttatcatata tatttagttt attatggata tatccacaag
     8401 ctgcaatgaa tcaatttggt ttttggttgg gaggagctgg tgcaatagga atattcttgt
     8461 ttttattctg taaccgtgca ctaataccaa ctgggttaca tcaagtttta aatgcttaca
     8521 tattatttga aatgggtgaa tatacaacat caacaggaga agttataaga ggagaaattc
     8581 ctagatttat ggcaggagac ccaaatgcag gattcttctg gacaggattt tacataatta
     8641 tgatgttttc aatacctgct atttcttatg ctatatataa aacagcacaa gataaaaaga
     8701 agcaagaagt aaaaggtgca atgaccgctg gagcattgac ttcttttatg gcaacagtaa
     8761 ctgaaccaat agaattttcg tttttatttg catctccaaa gttatatata attcattctt
     8821 tttattctgg tctaggtgga gttgtactat atttactggg tgctcgcttg ggttcattta
     8881 atggagcaag tataattgac tatgtcttaa gttttagtta tggagataaa gcgtggttag
     8941 ttatacctgt aggagttgta ttctttgtat tatattatgt aacatttaaa tatataataa
     9001 taaaagataa tgtgcaaaca ccaggacgtg aagttgagat agagataggt gataaagtta
     9061 gtgaaaaaga aaagaattta aaactttctc atggtaattt ttcatatatg gctaaacaga
     9121 taattaaaaa ttgtggtggt tatgaaaata tagtgactct taataattgt atgacgagat
     9181 tacgtttaga agtgaaggat gctactattt tgaatgatga taatattaaa aaaacaggag
     9241 ccaaaggggt aattaaattg agtaatacat cagttcaaat catcatagga acggatgttg
     9301 ttaaagtaaa ggatgagatg gaaatgcaac ttgatgagtt gagaaagcag gcagatgcat
     9361 aaaatgaaga aattagtagt tagggcagat gatataggat attcagaagg tgttacacta
     9421 gggataatat cttcttataa gaacggtatt gttacaagtg ctggaatcat ggtgaatatg
     9481 cctttttcaa gacaagctat agaaatggta aaaaatgatg atgtatgttt tggtttacat
     9541 gttaatttag taataggaga accttgttgc aaagaacata ttaagataaa gaatttgtta
     9601 gatgaaggtg gaaaatttgt ttctagcaaa ataagaagaa cgcaactact gacaggtaaa
     9661 gaggttttta attatgaaga agtatatgaa gaagtttgtg ctcaaattga aacttacaag
     9721 ttgtatatga gtaaattgcc tgagtatatt gatatacatg gtatagaaga agatgtatca
     9781 atatcagcaa tgtgtgatgc tgcaaaagaa tacggtatac caagttgtcc ctattattta
     9841 gattcgtgta ttagcatgcc aagtaaatat cctcagaatg atttttacaa gttaaataaa
     9901 tcttttgtta aattatttga agaaaaatat ataaatttag ataaagatat aacattgatg
     9961 gttactcatc caggatttat agattatagt cttataaaat catcttctct tattcaagag
    10021 agagtatatg aacatgcaat gctatgtgat aaggaagtta aaatgtggtt aaaaagaaat
    10081 aatattgaat tggttgattt taaagatatt ttcaagtcta ataattatat ataaagaata
    10141 gattacctaa agttttaatt aaaaataagt atttgaaaaa aatgtgtatt ttctaaaaaa
    10201 atattgatag agatgtatca aaataaaaac ttgaatcatc tctatcaata taggaagtac
    10261 acatttttta tgtataataa aaagagtagt atgcattaat aaattaaaaa tattaaatta
    10321 taattatatt aacgaaaatt ttagctaata tttttgaatt ttacttatga ataataaaat
    10381 agatggagga gaaactatgg aattttatga aagagcaaaa aaatatgagt ataaatttac
    10441 agatacagaa gacatgataa ttgaatatat gagaaaaaac aaagaagaaa tagtaaaatt
    10501 atcaatacaa tctttagcag ataaattttt tactgtccca aataccatag ttaggctatg
    10561 taaaaaaatg gagtatgatg gtttctctca actaaaaaat aatttaaaag aagagttaaa
    10621 aaatgagaat aaagagccaa aaaatggttt tgaatcagat ataaaaaaga cattagagct
    10681 aatagattat gaaaaaataa ataaagtagc agatataatt caaaaatcta ggagtattat
    10741 agtttatgga ataggtcaaa acattccttt atgtgagata ttagtaaaag aaattagagg
    10801 tgggcatgat aatataactt actatagtca aagacatgaa gtacttcatg atataggaaa
    10861 attaggtgaa caagatatgt tttttgcact tagtttaagt ggagaaacga aagaagttat
    10921 agaagcagca aagacagcag ttaataaggg ttgtaaggta gtagctctaa cacatatatg
    10981 tgaaaatcaa ttgcaacaaa ttgcagatat aagtttatat tgctattcaa caattaaaca
    11041 agtaaatggg tacaatatat cagataaggt tccaattatg atgattatga gagcaataat
    11101 agaacaatac tggttaagat agagatttgt gtataaatac acaaatcttt attttttaag
    11161 aagaaattgt gtgtataatt tcaaaaaaac attttcataa tttattttat gctatattca
    11221 tagcattaaa tgatttaagg agggcgtaat tttggaattt aagaaaatca caaatgaaaa
    11281 ccttgttttt ttaaaacaaa aaataaaaac aaagaaagac gcaataaagt tcttagcaag
    11341 aaagctatat gatgaaggca agttaacatc ttatgaggaa tatttagaag ctgtaatgta
    11401 cagagaaact ttatctccaa caggttttga aagagggctt gcaattcctc atggaaaatc
    11461 taagtcagtt aaagaagctg catttgcagc tatgacctta gagacaccaa tagaaaattg
    11521 ggaaagcgta gacccaaata ataaagtaga gataatattt ttaatagcag taccagaaaa
    11581 tgaaggtgga aatacacaca tacaaatact ttctgagtta gttactagac tgtcaaatga
    11641 agattatatg aatatgttgt taaattcaaa aactaagaaa gagttattta ataacttaga
    11701 tataaaagaa aagaaagaac aaataaaaga agagattagg attcaaaatg aatcaaaaaa
    11761 aattatatta gcaataacag catgccctgc aggtatagca catacatata tgtcagcaga
    11821 agcattaatt aaagctggta aagaaatcgg tgtggatgtg tatgtagaaa agcaaggagc
    11881 taatggtatg gtggaccctc atacaccaga tataataaaa agggcagatg caataatata
    11941 tgcaacagat gtagcaccta aaaatacaga aagatttgaa catttgccaa acataaaaac
    12001 gtcagtagca gctccactaa aaagagcaaa agaaataata tacgaagctt tagaagtagc
    12061 acaaaaacaa ggtaaaggtg attatataga aaaatcatct gatatatcat attgtgaaaa
    12121 atcatcatgg aaaaaagatg taaaatcagc aatacttaca ggagtatctc atataatacc
    12181 acttattgtg gcaggaggta tgatacttgc atttgcagta ttagcatctc aagcatttgg
    12241 tctacaagaa ctatacaacc aagaaggttc ttggttatgg caattaagaa agctttctgg
    12301 aggtatgtta ggtacattga tggttccagt attatcagca tatatgtctt actcactagc
    12361 tgaaaaacca ggtcttggac caggatttgc agcaggtcta gcagctgatt taataggtag
    12421 tggatttctt ggaggtatgt taggaggttt acttgctggt tatatagtaa aatttttaaa
    12481 gaagaatata ccagccaaag gcacattagc tggattcgta agtttttggg tatatccagt
    12541 agttggttct ttggtagtag gtttattgat gttatttgta gtaggaaagc ctgtagcagc
    12601 acttaacaat ggtcttataa attggttaaa tgggatgact ggagtaaatg caatactact
    12661 aggtgcaata ataggagcta tggtttcatt tgacttaggt ggaccaataa ataaagcagc
    12721 ctatacattt gcaataggag caatagcaag tggaaatttt ataccttatg caatatttgc
    12781 atcagttaag atggtttcag cttttggagt tacaatagct actgttttag gcagagattt
    12841 attttcagaa tcagaaaagg aaataggaaa gcaaacttgg atattaggat tggctggaat
    12901 aactgaaggt gcaataccat ttatgataga ggacccaatc cgtgttatac catcattggt
    12961 agctggttca gccataactg ggtctatagt agcattcttc aatataggtc ttcaagtgcc
    13021 tggagcgggg atattctcat tggcgctttt agagggaagt atgtcaggat ttattatagc
    13081 atcaatatgg ttctttgcgg cagtacttgg agctataata tcagcaatat tattaataat
    13141 aactaggaaa caaaaaaatc aaagaaagat acataaaaag atacgttatt agatatttaa
    13201 atcaaaggtt taattatagt gataaaataa atattaaaaa attttgtaaa ctcagtagta
    13261 tttttattgg atttttaatt agacagaaaa tattatcaga taagttttgg aggataagca
    13321 atgaaaaaaa atgtacacat agttcctcat atgcattggg atagagaatg gtacttctca
    13381 actgaggaat ctagaatatt acttgtaaat aatatggaag aaataatgga tatgttagaa
    13441 aataatcata actatccata ttatgtgatg gatggtcaaa ctgctatatt agaagattat
    13501 ttagcagtta agcctgaatg taaggagaga ataaaaaaat tagttcaaga aggaaggcta
    13561 ataataggtc cttggtatac tcaaactgat gaaatggtag ttggtggaga atccatactt
    13621 agaaatttac tatatggaat aaaagattgt gatgaatttg gagaatatat gaaaatagga
    13681 tatttaccag attcatttgg tcaaagtgct caaatgccac aaatactaaa tgggtttgat
    13741 ataaaatact caatgttttg gagaggatgt tctgaaagaa aaggaactaa taaaacagag
    13801 tttaattgga agagtgatga tgggtcttct gttttggttc aaatacttcc attaggttat
    13861 gcaataggaa aatatctacc aatgaatgaa gatgagctaa gaacaagaat ggataaatat
    13921 ttaccggttc ttgataaggg tgcaactact gaccatataa tacttccaaa tggacatgac
    13981 cagatgccaa ttcaaaagaa tatatttgaa gttatatata aattaaaaga atgttatcca
    14041 gagagaaaat ttttcttaag tagatatgaa aacatattta aagagctaga aaaaaatgag
    14101 gatatagata caataaaagg ggaattttta gatgggaaat atatgagggt tcatagaagt
    14161 atattctcaa caagaatgga tataaaatca gcaaatgcta gaatagaatc aaagataacc
    14221 aacatattgg agccactagc ttctattgca tattctctag gatttgaata tcatcatgga
    14281 ttaatagagt taatttggaa agaaataatg aaaaatcatg ctcatgacag cattggatgt
    14341 tgttgttcag ataaagttca ccatgaaata atgaatagat ttttcttagc tgaggaaaaa
    14401 gtagaccaat taataacatt ttacaaaaga agaatagttg atgcaatgag ttgtgaaatg
    14461 gctttagata agttagtagc atttaactta atgccttacg ataggaatga aatagtgaac
    14521 gcccaagtta taactaaaat gaaagcattt gaaatatttg ataaagataa taataagttg
    14581 gattttgaag tggtacataa agaagtaata gatgcaggat taatagaccg tcaaattgtt
    14641 cattatggaa actatgattc atttatggta tatacaataa actttaaaga taaaatacct
    14701 gcaatggggt ataaggcgta tatgataaaa gaaattggac atatgttaga aaaagcttat
    14761 gaagtatgtg acatggtaga taatgacttt tatactattc aagttaatga aaacgggaca
    14821 ttaaagatat ttgataaaaa attaaataaa acttttgaaa atgtattatt gatggaaaat
    14881 ggaggagatg aaggagatga gtatgatttt tcaccacttc caaatgagaa attaatattt
    14941 aacgataata tagtagcaaa ttccactata aagaaaaata gatttaataa tgagataaag
    15001 ataaattata gattaaaagt accaaaagat atacaagcta gaaaaaataa tagagcagat
    15061 tcatttattg actttgatat agttataaat gttccaaatg ataaagctat aatagatgta
    15121 gtattcaaca taaataacca agcaagagat tgtagggtta gaacttatat accaacaaat
    15181 atagcttcaa agttttctgt gtctgataat cagtttggac atataaagag aagtgtgtat
    15241 gatgatgcta tggaagtttg gcaacaggaa gaatggagtg aaagaccaga ttctatatat
    15301 ccaatgctaa cattcgttgg attgtctgac aaagagcatg gagtagctgt tcttacaaac
    15361 agtacaagag agtttgaaat agtaggggaa aaatttgaca caatagctat aacattattt
    15421 agaagtgtag gattcctagg taaagaagaa atggcaagac gcccaggtcg tccatcagga
    15481 ataaagttgc ctactccaga ttctcaaatg ataggagata taaaaataga cttagcaata
    15541 gttactcatg aaaaatctac actagaagca aatgttgcaa atatggctaa agagtattta
    15601 acaaaaatac aaacttataa taaaatgcca tataatgcta tgaaattgaa tcaatcagaa
    15661 atagaagtag attattcata tagtttactt aaggaaacta ataaggacct tgtgttaagt
    15721 gttgtaaaaa aggctgaaaa acaagaggca ttagtagtta gattttacaa ttctaaagaa
    15781 gaaacaaaat cagctaacat agtatttaac aaaaatatta aaacagctaa atggattaac
    15841 ctaaatgaaa aagaattaga atctatatca atagataata actttgtaaa tgtagagtgc
    15901 ttaatgaacc aagttaaaac tatattggtt aaatagtgct aaaaaggaat tgtcttaaaa
    15961 tagagataat cctccttatc attgttgaaa taataaacat atatttgatt ttatacaaaa
    16021 agagtgtctc atgaactaag aaatttattt tgagacactc cctttttata agataaaaga
    16081 atattaatgg tataagttgt atacaaagag agatgtagag tataaaataa aagataaatt
    16141 aagcttataa aattatatgt ttaaaataag gagtaataaa aaggagtaga taagtatgaa
    16201 aatattaatt tctatagatt ctttgaaagg aagcttatct tcaatagagg caggaaatgc
    16261 aataaaaaag gggatattga aggttaaaga agatgcgcaa gtaaagatat tgccacttgc
    16321 tgatggtggt gaagggacag tagatgcttt agttcaaggg atgaatggta aaaaagagac
    16381 tatagaagtt actggaccaa tagctaaaaa agtggatgca acatatggat tattaaaaaa
    16441 tacaagtaca gcaataatag aaatagctca agcatcagga ctaacattgg ttccgactga
    16501 acttagaaat cctttataca ctacaactta tggtgttggg gaaataataa aagaagcaat
    16561 aaataaaggc tatagaaatt ttatagttgg gataggtgga agtgctacta atgatgctgg
    16621 gataggtatg cttcaagcct tagggtttga gttttatgat gaaaataaca agttagtagg
    16681 attaggtgga aaagttttaa atgaaataag acatataaaa atagaaaata gattaaagga
    16741 attagatgaa tgtaagttta aaatagcctg tgatgtaaat aatccgctgt ttgggaaaaa
    16801 tggagctgca tacatatacg ggtctcaaaa aggtgcaaca tcagaaataa ttgaggagtt
    16861 agataatggg cttagaaatt tttccaaagt agttaaaagt tacttaagta aagatgtagc
    16921 taatgtagaa ggagcaggag cagcaggagg attaggtttt gcttttttag catttttaaa
    16981 ttcaaaatta gaatctggaa taaaaataat attagaagaa attaaattag aggaagagtt
    17041 aaaagatgca gattttgtca taactggtga aggtagattg gacaatcaaa ctgctatggg
    17101 caaggctcca ataggagttg caaaactggc aaaaaaatat ggggtaaaag taataggttt
    17161 ggctggagcc acaacagaag atgcagttaa atgtaatgaa gagggtatag atgcttattt
    17221 ttctatagta aatagagcta tgacaataga agaagcaatg gataaagcaa ctgctagtga
    17281 gaatatgaca tctacaacaa cacaaatatt caatcttata actagtatac aacagtcaaa
    17341 aaatatgtag tattaaaaca aggtatatta ggtaaaaagc tgtcacatat taatttagac
    17401 tttttagtgt gacagcttat ctattttatg ttaataactt aagaaacata tattggaaat
    17461 aaattaacgg atttagatat tttgaataag ataagctatt ctattcataa aactactata
    17521 gttatctgcc ttagtaagtt ctttaacatt attccactca gataatattt caataaagct
    17581 ttcaaatatg taattgaatt tttctttttc tttttcattt ataggtatca tagctataat
    17641 ctgaactttt tcctcacccc atttaactgg tttatcgtta acaattaaac atactccagt
    17701 ttttaaagca tccattttca tgctatgagg aattgctaca ttgttattaa atgcagttga
    17761 agacattttt tctcttataa gaacatcatc tatataattg ggtttgacat atttattttc
    17821 gtacagaata tttcccataa attttatata atctttagca ctatctaaat atatattttt
    17881 cataaacaaa gattcactaa agtacatttc taaatgattt tgtacagtaa gtatattttt
    17941 tttatcttta atctgaataa atttactttg gatattatca taatcctttc tatttaggta
    18001 aggatttaca taaacaaaag gtatagggca tctatcgtta attggtaaag tagtaataac
    18061 catgtctacc ttattgtcaa aatcaagatt atgagtatct gaaaaaatct gaataatttc
    18121 tatggattca ttaaatctct tctcgaggtc tcttactatt ttgaattgta aatcatagta
    18181 atttggagta attaatgcac atagaacttt atcttctaat aatttttgtc tatcaaagaa
    18241 tgaacctaca tgaagagata tataagatat ttcatcttcg tctatatcta cattttctaa
    18301 agtttgaatc tgattgcaaa tataaacaga tatatcatat atcaaagggt aggaatcttt
    18361 taacttttga ggtatttgat ttcttaaaac ttgattattt ttggctctac tgattaaatt
    18421 ttttacatga agagtaaatt ttacaacaaa ttcctcatca gataaatcta taagatataa
    18481 gtcatataca tttttaatta tttttttagt taatttaaca aagtgatcat ctatataatt
    18541 tggcaatgac ttagtatcca tttcatgata atttaagact gtggttttgt ttgttaactg
    18601 aaatactaag tagtataatt ctgcactagt taaagttata ttgtattcag actctaaaaa
    18661 atttgatata tctcttgaaa tgcttatatc attattatta tctatgtagt ttactacttc
    18721 gacagattct atgtagttat tcttttttat tctgtctata gttatcatta aatgaagaag
    18781 tatgttattt atagcatact catttataaa caaattgtat ttttttaata tatatattat
    18841 attttctttc agtttggtca aattatactc ttgatatatt ttttgtacat caaataagct
    18901 taaaaaatca cttcccgttt ctttaaatat tatagagctc attaatttgc gctttgcaga
    18961 ctcatttcca attagtttca ataaaaattt cgattgcttt atctttaaat tataacttcc
    19021 tataaattta ttagctttga ctatatcccc ttcaatagta gctgggctaa caaacaattc
    19081 ttcacttaaa tcaaatatat taattccatt actattaact ataagttctt ttaaaattaa
    19141 atttaacctg ttatcaggtg atatattttc tgaactatca accttggttt gacaattatt
    19201 attgagattt actttatatc cttttttaga ggataatata aattcaccat tggaattaat
    19261 ttcgtttaca tattttctaa ttgaacgcgt tgagacatta agataattag ccaatgtaga
    19321 tgaatctatc caatcatgtt gtgtttttat gaaatttaaa aatagttctt ttttagatgt
    19381 tttcattaga ttccctcgca tagttttaaa ataaaatctg gatgatatga taattatata
    19441 attgaatgta cgtattataa gatacattat actatattaa tatgtttaat taagagtaac
    19501 tgatttccta ttattaggaa atatatttat gtgaaatgaa cttatttggt aagtacaata
    19561 taaacataat aaaataaaaa gttcattaag aaataaatta atggataaaa atattggagg
    19621 tattattatg aaaaatataa gagtgctttt ggcgtgtggt tcaggagcaa gtacaggttt
    19681 tatggcaact agtatgagaa agagtgcaaa gaaaaaaggt atagaagcag atataaaagc
    19741 gagaagtgag tcagagatag ataagtattt atcagaaata gattgtttat tactaggacc
    19801 acatttagat tatatgaagg aagaaattca acaaaaggct aatgaatata acgttccagt
    19861 agcatgtata tctaaaactg catatggaag actagatggt gcaaaggctt tggaagaagc
    19921 actagaacta atatcaaaca aataaaatat ttatggggag agtgtttatg aataaattta
    19981 tagatttttt aagtgaaaaa tttactcctg ttgtaaataa tatgactaag aatatatggg
    20041 tacaatcagt tcaatcaact ataatgaaag ttttgccaat ggtatttgta ggctcattag
    20101 taactatagt ttctgtcttg aagaattaca taagcttttt acctgattta agtccaataa
    20161 atcaatatac atttggactt ttaggattat ttatagcatt tttattgcct atggaaataa
    20221 tgaagaataa gaaatttgaa agtatgagtg tagtcgctgg tcttgcaggt gctggattaa
    20281 tgttaatgat gattcgtcca gagattacga atgaaggagc tatatttaac ttcaacaggt
    20341 ttggaggaga aggaatgctt gtatcgctag ttgcaggatt attctctgga ttaataatgt
    20401 ccttatttgg ttctttttca tttttcggag aagattcagc acttccggat tttgtaagca
    20461 agtggtttga taatatgtta cctataacag tgataatgtt tgtaggctgg ggattaattt
    20521 atggactcaa ctttgacatg tttgaaataa tagttagagt atttaaacca gtatcagata
    20581 tagcacagac attacctgga tttgtactct taaacttttt agtagtattt atatattctt
    20641 taggaataag tgggtgggta ctatccccta taacttattc aattcaactt ggtgcaattg
    20701 cagctaatgc agcagcagta gcatcaggag gagtagcaac taatatatat gtttacgaag
    20761 ttattcaaac aggttggata actttaggag ggttgggtgc tacattacca ttagttataa
    20821 tgatgtgttt atccagagtt ggaagaataa aagcaatagg aagagcatgt attgtccctt
    20881 ctataatgaa tataaatgaa ccagtaatat ttggagctcc agtggcatgg aacccatttt
    20941 taatgttacc tatgtggata ggtacaatag taatatcaat aactacttat ttatcaatga
    21001 gtatgggatt tgttacaatt ccaagtaagg tgtttaattt atggtatgtt ccttttggta
    21061 tatctacttt tatggtaaat caagatgtta gaggtcttat tgttttagca ataaatatag
    21121 tattgctatt tatgatatgg tatccatttt ttaaagctta tgagaaagaa gagataaaat
    21181 tagagagcca agaggtttaa atttagaaat tgaaaggaaa ttataaatat gacaattgaa
    21241 gaacatataa tagatgaaga attagtagag gtggcaatgc aaataatact tcgtgcaggt
    21301 gaggctagaa cagaaattaa acatgctctt aatgatttgg aaaagtttga ttatagaaat
    21361 gctgatttaa aactagctaa agcaaaaggg tttatgacag aagcacatag agctcaaacc
    21421 aagattatac aaggggaagc tgatggagag aaaagagcac attcgctgtt atttgcacat
    21481 gctcaagata ctcttatgac catattcagc gagttaaata taactatgtc tttagtagga
    21541 atagtaaagt ctatagaata taaggtaaat aatagtaaat gatttaaata aataaaataa
    21601 ggagtgatta aattgggtaa gtttcctaaa ggttttttat ggggaggagc tgtagcagct
    21661 aatcaattag aaggtgcatg gaatgaaggt ggcaaaggtt ggtgtttagc tgatgtacat
    21721 atgtatgata aagatagaga tatatctaaa ccttatgagt ctgatatgac tatagaaaga
    21781 ataaagtttg ctatgaaaga taataatgga tattatccta agcgtagtgg aatagacttt
    21841 tatcatacat acaaagaaga tttaaagctt ttgaaagagc tagggttaaa gtgttttaga
    21901 ttttcaataa attgggctag aatatttcca aatggagatg atttagagcc taatgaagag
    21961 ggattaaagt tttatgataa tcttataaat gaagtaatag ataatggtat ggaacctatt
    22021 gtaacactac ttcactatga gactcctttg aatttagtat taaaatataa aggatggtct
    22081 aatagaaaag tggtagattt ctttgttaga tatgcagaat taatactaaa tagatataaa
    22141 aataaagtta aatattggat agttataaat caaataaatt taatacatta tgaatcattt
    22201 aattcagttg cattttgtgt agaccaggta gatgatgtag aagaggccaa atatcaagct
    22261 attcatcatc aattcatggc tacagcttta actaagaagc ttggaaaaga aataaattca
    22321 gatatgatga tagggatgat gatggcagat tgtacatctt atccagaaga ttgcgaccct
    22381 gaaaatgtgg tgtttgcttt aaaaagaaat agaatacaat attttttcac tgatgtaagt
    22441 tttaggggag agtatccagt ctatattaag agatttttca aagaaaataa cataaatata
    22501 gttatagagg aaggagatga taaaatctta aaagaaaata ctatggattt tctagcttta
    22561 tcatactatt attctacaac agtatcagca agtaaaaaca ccatgaatcc aactgataat
    22621 ataaagaatc catatttaaa agcaaatcct tgggggtggg caattgaccc aaaaggatta
    22681 tacaatactt tatctcaata ttatgataga taccaaaagc caataatgat agctgaaaat
    22741 ggatttggaa tgcatgataa atttgaagat ggaaaagttc atgatagtta cagaatagat
    22801 tatttaggaa aacatataga acaaataggt gaagctatag ctgatggaac cgaaatatta
    22861 gcttattgta tgtggtcgcc aatagatata gtaagttgtt catctcaaga aatggaaaaa
    22921 agatatggat ttatatatgt ggatatagat aacaatggaa atggaagtaa agaacgttat
    22981 aaaaaagata gtttttattg gtatcaaaat gtaataagaa ctaatggaga agaattaaga
    23041 gaaaagtaaa tagtttttaa atataaacaa ccccccttat aaatactgta tatattatta
    23101 atatagtttt ataagggggg ttataattgt aaataaaatg atttattaat tatatgattc
    23161 atagcgaaat ttatatttat acttttaatc ataaacatat cagtatgagg aatatctatt
    23221 gtacttcatc caaattttgg tagaggtaca attctttttt attttaaaat gctataagat
    23281 aacataataa actttaagca aaaaattatt atattataaa tttacatata tagacattga
    23341 ctaaatctta attgtatata aaaataagta gttaagaggt aatatattat attactcgat
    23401 aaatttagac aaataaaact tatataaggg gagataacat atgataattg agctaaacaa
    23461 aagtaattct gaaaatctaa cgaagacgga acttgaaata attaaattta taaatgaaaa
    23521 tgaatcaata ttaccagaat tatctatagt agagattgct catgaaacat attcatctcc
    23581 agcaaccgtt tcaagagcaa ttagaaaatg cggtttaaat ggattcaatg aacttaggta
    23641 tagattaaca gtaaaagatg aaaatgatga tatacaaaat atgggggata taataaataa
    23701 atcctttata gaagcacaga gagttataga acaaatatc
//
LOCUS       DAKBNN010000042        23302 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000042 DAKBNN010000000
VERSION     DAKBNN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.042"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..669
                     /locus_tag="OUB02_001436"
     CDS             <1..669
                     /locus_tag="OUB02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8885202.1"
                     /translation="VYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGH
                     GGSAPGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSL
                     KPDLFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIK
                     TRVLPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIE
                     DSLK"
     gene            911..2824
                     /locus_tag="OUB02_001437"
     CDS             911..2824
                     /locus_tag="OUB02_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885203.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFGDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            2817..5036
                     /locus_tag="OUB02_001438"
     CDS             2817..5036
                     /locus_tag="OUB02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCT8885204.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            5145..5975
                     /locus_tag="OUB02_001439"
     CDS             5145..5975
                     /locus_tag="OUB02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCT8885205.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            5965..9432
                     /gene="addB"
                     /locus_tag="OUB02_001440"
     CDS             5965..9432
                     /gene="addB"
                     /locus_tag="OUB02_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCT8885206.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISTLEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            9432..13259
                     /gene="addA"
                     /locus_tag="OUB02_001441"
     CDS             9432..13259
                     /gene="addA"
                     /locus_tag="OUB02_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCT8885207.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            13305..14516
                     /locus_tag="OUB02_001442"
     CDS             13305..14516
                     /locus_tag="OUB02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCT8885208.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            14503..18030
                     /locus_tag="OUB02_001443"
     CDS             14503..18030
                     /locus_tag="OUB02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8885209.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            18161..19414
                     /locus_tag="OUB02_001444"
     CDS             18161..19414
                     /locus_tag="OUB02_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCT8885210.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            19563..20615
                     /gene="ytvI"
                     /locus_tag="OUB02_001445"
     CDS             19563..20615
                     /gene="ytvI"
                     /locus_tag="OUB02_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HCT8885211.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            20706..21932
                     /gene="pepT"
                     /locus_tag="OUB02_001446"
     CDS             20706..21932
                     /gene="pepT"
                     /locus_tag="OUB02_001446"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCT8885212.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            22139..23191
                     /locus_tag="OUB02_001447"
     CDS             22139..23191
                     /locus_tag="OUB02_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HCT8885213.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISHSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         9941 a         2179 c         4168 g         7014 t
ORIGIN      
        1 gtatacaaaa taggaggagg attgacttct aaggtaatga gctctatagc atcatcatta
       61 tctaaacaca atacgactcc aacagaacca ggaaatagtg ggggcaagac agttatgatt
      121 gacccagggc atggtggttc agcacctgga aattcatctg gaggaatgat tgaaaaagat
      181 tacaatttaa atacttcact tgcaacaact gaatatttac gttcaaaggg attcaatgta
      241 ataatgacaa gagacacaga taagacttta tctcttggaa atagaactgc tctatctaat
      301 tcattgaaac cagatttatt tacaagtata cattataatg gctcaactaa taaacaaggt
      361 catggtgtag aagtatttta taagcttaaa gataaaaatg gagggactac taaaactgta
      421 gctaccaata tattaaatag aattttagag aaatttaaac ttacaaatag aggtataaaa
      481 acaagagtac ttcctagtga ttctacaaaa gattatttat acgttttaag aagtaatgat
      541 atgccagctg tacttgtaga atgtgcattt ttggataatg aaaatgatat gagtttaata
      601 aactcatctg caaaagtaaa agaaatgggt acacaaatag gtaaaggaat agaagattca
      661 ttaaaataaa tggaaataaa aatatttaaa aacttaatgt aaatttatag ttgtaaattt
      721 atactaaagt caaaaaatga aatataagtg tatcaatagt aagactgtat gtacatttgc
      781 acatgtgaat gcaagtatat atacagtctt tttttgtagt taaatgcaat aatttgattg
      841 tgttttgtaa aatacacaat atgatataat tagataatat acacttaatt taattgagga
      901 aggtgattat atggaatatt tatttgaaaa aagtagttta aaagaatatg gaaatgaagc
      961 tttagataaa tatagagaat tattagataa aactggtaat aattcaaggt gtattctttt
     1021 gttagtacca aacaataaca caaagatacg atatgaaagg gctctaagat taaattatag
     1081 tgaagaactg aaaataacaa catatataag ttttgtgaag aaagaattag ttaaatactg
     1141 gccactaata atagaaaaat gtgatgaaat aagtacaaag gttgtatctc caacatttat
     1201 atcaaatagt ttaagtgaat atttaatagt gcaaaaagtt aaggaaaaaa ggatacaaga
     1261 aggatatttt ggagatatta caggaaccaa taaatccata gcaaatagta taatgactaa
     1321 cataaataag tctgcattta atttaattga ttttgatttt attggagaaa aaatatattc
     1381 atcaaagaaa aataaagata gtatatatag attttcatac actcaaatgg atgaaattat
     1441 atcatattat ataaattcct tactatcaaa ttcaatttta gataatgcat taagcatata
     1501 tttatacaat caatacctac ttaatgatga tttttattta aaaaacttat ttacagaagt
     1561 gagatatata attgtggata gccttgaaag ctcaagtaat gcagaagttg attttataac
     1621 agaagcattg gataatacat tacaaagtta tatatatttt gattatagta gggattattc
     1681 tgtatttaat aatgtggata tagactatat aaatgagaaa ataatatcaa aaattaagcc
     1741 taaagaagaa aatattaaaa agaaggagat tagaatagaa gatttatact tattacctgc
     1801 aaatatagaa ttaaatcaat caagtcaatt atataatgaa atgttagatt taataagtga
     1861 aaaagttata tctcttatag aagaagatgt atctccaaga gatatagcaa ttatatctcc
     1921 tataaacaat agtatattgg agcatcaaat tagagattct ttgatagaaa aaaatataga
     1981 tgtttttagt actaagaagg ataaaaaagc tgtggattat ccatatggga atgcattggt
     2041 agtggctaca tgtatattct atggatattt agattttata aaagatgatg aatacttaag
     2101 ctttttagaa actcttttag aaataaatag gataaaagca tttaagattt ttaatgaaac
     2161 aagacattta agtattgata atatagatga acatagtcag tatcgtgata ttttacagta
     2221 tattgaagaa aaaaagaaga gtgatataaa aatacatgaa tttttaactc aattttacat
     2281 agataaaatg ttaaatttaa aagaaggtaa aaagaatgtt gggttatgta aaaagataat
     2341 agctgaaagt gagtctttta gtgaaagttt aagtctactt gggatgaaaa aagaaaagat
     2401 atttatagaa gctttaaaaa gtacaataaa cgattattac tctgttgtcg atatagaaga
     2461 gttgaaatcc aaagacaaaa tagtaataac tactccttat tcatatatat catcaaatat
     2521 agatagaagt atacaaatat gggtagatat aggaagtaat gcttggaata tgaaaataga
     2581 aaaagatata agtaatttaa ttgtacttag aaaatctttt gaagaaaaaa agatttatac
     2641 aaatgaaatg gaagaatatt ataaaaaata ttatctatac aatacagtat ataatttgtt
     2701 attaaatgca aagaaaatct atgcatttaa aagtgaatat gcgattaatg gttatatcca
     2761 agagagtata ttgtatagta tacttttaaa aatctcacat aaaggagata aaaattatga
     2821 ttaatataaa ttatagagaa gaccagatac caataataaa ttacgataat ggtactatgg
     2881 cagtaccagc agtaccaggt gctggaaaaa catttataat aacaaattta gtagctaaac
     2941 tattacttga acagaaacat aagggtggta aaatattaat tcttacatat atgaatagtg
     3001 ctgtaaataa ttttaaaggt agaataagaa agattcttga agagaataaa atagatgata
     3061 ctaattcgta tgaagtaatg actatacaca gtttggctgt aaaaataata aaggaaaagc
     3121 cagaagttgt aatgttaagt gaggatttta atatagctga tgatttgcaa aaaagtatta
     3181 tattaaatga atgtataagt agatttagat ttgaaggtgg agaaagagct tttagatggt
     3241 ttttaaagga gcaaaaagat gaacgctgga aagaaataac tcttgaagcc tgggaaagag
     3301 gtttttttga atttgcagga aatgcaataa gtgaacttaa gtataaggga atttctccag
     3361 ataaacttga agaaatattg tcaagaggac acaaaggaat gcttaaaatt attttaccaa
     3421 tatataaatt gtatgataga aaattaaagc aaaatggatt gctagattat gatgatatat
     3481 taattttagc agaaaaaact ttaagcttag acgaagggct tagaaagaag tttcaaacta
     3541 gatacaaata tatatttgaa gatgagtgtc aggactcaaa tgaaattcaa ggtaatataa
     3601 taaaaattat atctagcgaa aataaaaacc tagttagagt tggagatata aatcagagta
     3661 taacaggaac attttcatca tcagacccta aattttttaa agaatttata aatagtgcag
     3721 atttctgtta taggatggat atgtcaaatc gaagttcaaa agatatactt gatttagcaa
     3781 acactcttgt caagtatgtt acaaaggaat ttagacagaa agaatgtaga catgctcttg
     3841 aagatatgca aataaaaact gtgccaaatg gaatgggata taaagaaaat cctaatccag
     3901 aacattataa tataaatgta aagtggtatg agagttggaa aattgaaata gagcaaactg
     3961 ctagatacgt taagggtata aaaaataaat atccagataa aagtataggt atattggttc
     4021 catttaatga acaagtgaca caagttgcta aagaacttag agagaataat ctaacctttg
     4081 aagaattagg accaaattct ttgaataaaa ggaaggttat aaacaatata gcatatatta
     4141 ttgattttat gttacattgt gatgatgtag aaaagttaat agttgtactt aataaagttt
     4201 ttatagatac tgataatgaa gaagaaataa agtatttttt agaagaatta aaaaactatt
     4261 ctaccgaaga aattatttat aattttgaat ttagtaagtt agataatgat ttaatagaat
     4321 ttaaagaaga aagtgatata tacttaagtt ttaaaagagg aatagatatt ataaaacaga
     4381 tattagaata tccaataata aggttagacc tcttagtttt gtttattggg aaaaaactta
     4441 atttagaaaa agaagataaa gctgtactgg attatatatc tttttatata aagtatctag
     4501 tatcagaaaa tataaatatg gatttagaag atgtatataa tttattgttt gatgtaaaga
     4561 acagggtgtt taatcatata atagatgtgg tatatgagat aaatggatat gaaccagagc
     4621 ccggtagtat aacactatgt aactatcata aatcaaaggg gatggaatgg gattgtgtgt
     4681 ttttactagg tcttgttgaa tataatttcc ctgacaatat taatcaaaag tttcagagtg
     4741 ataaatggta tctaaaagaa aaatataaaa accctatggc aattattaaa tcagaagttg
     4801 aagctatatt aaaaggaagt atttcaacag attatgctca taaaacgaag attgattcta
     4861 taaatgaaaa gattagactt ttatatgtag ggattactag agcaaaggaa atgttagtac
     4921 tttcaggaag tgcttataga gatgaaagtg atatagggaa taagagaaaa gagcaaaaac
     4981 catgtatata tttaagcaga cttaatcaac atattattga aaaaaggtca aattaatata
     5041 taataagtta ttaaaagatt gcttgaaata tatctaaaag gggataaaac aatatgtaca
     5101 gtaagtaact actaaaaaat aaagttaaga gagaagtgat atgagtgaac gataaattaa
     5161 aatatttttc ttatagtcaa aattcaatta acacatataa atcatgtcca ttgaaattta
     5221 aatataaata tattgataga ataaattgga aaaatgatga tgttggtagt agagagtatt
     5281 atgaaacttt aaaaacagga agagattttc atttgatttg tgaaagatat tttagcaata
     5341 tacctcttgg aatctatttt aatgaggatg ataaaaatag taaaaagttt ttaaagtgga
     5401 tggaaaatat aaaaaaagtt gttcctattg aaaaaggaaa aacgtatttg ccagaatatg
     5461 aagtaaggat gacattaaat ggggatataa tacaggcaaa gtatgattta gttgtaattg
     5521 gaaataacaa tatagaaata tgggattgga aaaccgagtc taaaaagatt gactataaac
     5581 acgtagaaaa tagaatacaa actattgttt atatgttttt agcaaaagaa gtaataccta
     5641 aagttttaaa aatggacata aatgttaaag atataaatat gagatattat caacctgaat
     5701 ttgatgactt accaattacc attttatatg atgaagaaaa acatgaagca aatagaaata
     5761 aaattcaaaa ttatataagt atgataaaaa acactaatta tgaagaacat acatacgaag
     5821 acaatctata caatgatata gaaaaggtat ataataaaga agaacgtatg tgctatagaa
     5881 ataaaaagca ttgcaagtat tgtgaattta ataagttatg taatggcaaa gaaatagatt
     5941 atagtatttt ggaggcggaa atttatggga cttaggtttg tattaggtag aagtggaagt
     6001 ggaaaatcca cctatatact agatgagatt aaaaaagaag ctcaaaaaaa tgaaacgaca
     6061 tcaattatac ttttagttcc agaacagtac acatttgagg ctgaaaatag agtaagtaag
     6121 ctattcttag gaaaagaaaa agataagtat ctaagagtta gggtacttag ctttaagaca
     6181 ctaagtaata tagttttttc tcaagtagga ggattgacag atgtaaatat aaattctagt
     6241 ggaaaagcaa tgatggtata tagagctatt gaagatgtaa gtgaagaatt aaatgtgttt
     6301 tcaaaatcaa aatcgcagtc tggttttgta agttcaatta cagatatgat atcagaaatg
     6361 aaacaataca atataagccc tgaaatgtta gaaaatatat ctggagaatt ggataatgaa
     6421 actttaagtt taaaattaaa agatataagt aaaatatata attcttttga agggaaattg
     6481 catgaaaatt atgtagatgc tcaagatatg ttaacttcat tggcaagtaa aattgaatta
     6541 agcagttatt tagatggagc ttgtgtttat atagatgaat ttacaggttt tacaccaaat
     6601 cagtacaatg tcataaagag tatattaaat aagtctaaaa gtgttaatat ctctttgact
     6661 gtagatgata tcaactatat tgggtacagt aaatctgata tgttttctag aacgaaattt
     6721 acttattcaa aattgactca attgtgcaac gaagaaggca taaaaatatt gccacaagta
     6781 aatttaaaca caggtgttat aaaaagattt gaaaaagtta aggaattaca gcacttagaa
     6841 agattttata atgcttatcc atataaaata tattcaaatc caactgaaaa tataaagata
     6901 aaagagttta acaatttata ttcagaggtg gaagagatag ccagagaaat tgtacattta
     6961 gttagagata agaatgtcag atatagagat ataaccattg ctacaagaga tttaaataga
     7021 tatgattttt tagtacactc tatatttaat gaatataata taccaaactt tatagataag
     7081 aagagagagg caaaaagtaa tccaatagtt attttaatta tttcaacttt agaaatgaag
     7141 aatagacgat atgggtatga aactatgttt aggtatttaa aaagtggact tataggaatt
     7201 gataatgatg atataaattt actagaaaat tatgtgttgg ctaatgggat taaaggtaaa
     7261 aaatggtttg atgaaaaatg ggattataga ataactcaaa gtctttcagg tcaagaaagt
     7321 gaatttgaac tggaattaaa agagaaaata aatgaaataa aaaatagagt attagagcct
     7381 atagtaattt tacaagaaaa attaagagga aaaaatagag taaaagaaat ctgtagatat
     7441 atatatgaat ttttattaga tataaatatg ccagaaacta tagaaagttt aatagtaaat
     7501 tttaaggata aaggggaact tgatgttgca aatcaatact cacaagtttg ggacatagta
     7561 gtggacatat tagaccaaat ggtggaactt atgggtgatg aaataatttc tttagaaaaa
     7621 tttataaaat taataacctt aggttttgat gaatatgaat taggactggt tcctcctagt
     7681 atagaccagg tacttgtaag tagtgtggat agaatgaaaa atccagacac gaaatattta
     7741 tacctagttg gaactactga tggtgtgttt ccgctgatta caaaggatag tggaatattg
     7801 agtgataatg atagggaaag tcttggaaac aaaggaatcg aagttgatat tgatagtaag
     7861 actaggagtt ttgaagaaca gtttcttgta tataaagctc ttacttcgac atctaagaat
     7921 ttaaccataa catatccaat atcagaccat gaaggtaaaa cgcttagacc atcaataatt
     7981 atatcaagac ttaagaagat atttccaaat atagaaaata aaagttattt agtggaagaa
     8041 aataaaagca ctgataaaga tatacttaaa aaaataacgg ttaaatcccc tacatttaat
     8101 gaacttataa acataataaa aaattatgac agtgatggtt acaatacaga agagattaat
     8161 agtatttggt tagatatata taggtactat ttaaaagatg aagtctatag ttctataaca
     8221 aaaaaagtta taaaaggtct tagttatact aatcaagttc ataagattga agagaaaaaa
     8281 ataaggtcat tgtataaaag taattctctt agcatatcta ggcttgaaaa atatgctgaa
     8341 tgtccatttg cttattttat acaatatggt ctaaaagcaa aaaagcgtaa agagtattca
     8401 tttacaccac cagatttagg aacttttatt cataatattt tagataggtt ttcaaaagaa
     8461 cttttacaag ataatttaac ttggagagat attgatgaaa aatatataga attaaagata
     8521 ggtataatag ttgatgaaat aattttaaag ataccaggat atatattaaa tagttctgag
     8581 aggtataaat accttgcata tagattaaag aatatgttga caactgctat aactataatt
     8641 agtcagcaaa taaaacaagg ttcatttgaa ccaatagatt atgaagttaa atttggcaac
     8701 aatggaaaat atccacctat aaaaatggtg ttagaaaatg gacaagaagt aagtttaatt
     8761 ggacaaatag atagagttga tgaatttgaa gaaggagaaa ataaatatat aagaataata
     8821 gattataagt caggcaataa gtccataagt ctaacagaaa tatattatgg tctacaatta
     8881 caattacttg tgtatttaga tgcaatcctt gaaagtgcaa aggatgaaga tataaatata
     8941 aatccagctg caatactata ttgtagaata aataatccaa tagcaaaatt taatgaagat
     9001 aaagatgatg aagaaataca agaagcaata ttaaaagaac ttagaatgaa ggggcttgta
     9061 gtaaaagact ctcacatagt taaagagatg gataaatcat taatagatgg agaaaggaaa
     9121 aactctttag taataccagt aggtcttact aaggatggaa atgtaggtaa aagtacttca
     9181 gcaataagct atgaggattt caaactgctt agaaaatatg taaggcatgc tataaaggat
     9241 ttatgtgaag aaatgttaag tggagaaata cgtatagctc catataaaca taaagatgga
     9301 acatcatgtg acttttgtga ttactctgct atatgtcagt ttgattcaac tataaaagat
     9361 aacaagtata aaaatttaaa taataagagt aatgaagaga taataaaaat gatgaaagga
     9421 gatgttaatt agtgagttct cctaaatgga caaaagagca attagaagtt atagaaagta
     9481 gagaatgcaa tttattggtt gctgctgctg ctggttcagg aaaaacagcc gtgctagttg
     9541 agcgtataat tcaaatgata acaagtagag aaaatccaat tgatatagat aagttattag
     9601 ttgttacatt tactaatgct gctgcgtcag aaatgagaga gaggataggg gatgctatag
     9661 gaaaagcctt agatgaaaac ccagaaaata aacatttaca aaaccaactt gttttattaa
     9721 ataaatctag tattacaaca atacactcat tctgtttgga tgttataaaa tcaaactttc
     9781 atagaataaa cttagaccct aatttcagaa taggagacca aactgaatgt gctattttga
     9841 aacaagaagc aatagaggaa gtatttgaag atttatatga agaaagagat gaggggtttt
     9901 taaatttagt tgaaagttat gcagaaagag gaggagataa agaagtacaa gatattatct
     9961 tgggaatata ttcatttgca atggcttctc ctgaacctaa aaaatggttg attgactcag
    10021 cagaacgatt taatattgat gaaaattttg atttttcaca gtctatttgg gctagagcta
    10081 tattagacac tgtaaaaata gagattaatg gtctttgtct aaatatggaa agggcattaa
    10141 aagaagtgga atcaatagaa gaattagaaa cttttgcaga aaagttatct gttgaatata
    10201 aaaaaattgc tgacatttca caagcatgta ataaatcttg ggatgaggca tataaaaaga
    10261 tggcaagtat gtcctttgaa aattatgtaa aaggagtaaa aagaatttca aaagatgctc
    10321 cttcgtatat aaaagaatca aaagaaaagg caaaaactat aagagataag actaagaagt
    10381 ctttagaaag tatagtaagt gctacattca ataaagataa tgattctatt agagaagaaa
    10441 taaaatatct ttataacata gttaaaccaa tatctagtgt tgtactgaga tttgaagagg
    10501 aatatagcaa taagaaaaga gaaaaaggaa taatagattt taacgatata gagcattttg
    10561 cattaaatat acttacagat gtagatgaaa aaggtaatat tgtaccttct gatatagcag
    10621 ttggatatag aaacaagttt tatgaaatat ttatagatga atatcaggat agtaatctag
    10681 tgcaggaagt actgcttaaa gctgttgcaa atacagaaac tccaaataga tttatggttg
    10741 gtgatgtaaa acagagtata tatagattta gacaagcaaa gccagaatta tttttgcaaa
    10801 aatataataa ctataatgat aaaaagggaa gttcccatag aaaaataatg ctttacaaaa
    10861 attttagaag tagagaagaa gttgtagatg ctgtaaatta tatatttgaa aatataatga
    10921 atgagaatat aggagaaatt gaatatacag aaaaagaaag actaaattta ggtgcaaatt
    10981 ttaatgtgga tacagacgag aaatctatta ttggtggagc aacggagata cacctaatac
    11041 aaaaagataa taaacttgat gatgatatta taaatgataa agacgataga attaataaca
    11101 aagaagatga aatagaggaa gaagaaaaat tagataatat acaacttgag gctagaatgg
    11161 ttggaaacat catcaaagat ttaatgaaag tcaatgaaga tggaaaaatt caaaaggtct
    11221 atgataaggg aatagatgga tatagacctg tggaatttag agatatagtt attcttttaa
    11281 gagctacatc tgcatgggct ccagtgtttg cagatgaatt gatgaatatg gatataccaa
    11341 catatgctga tgttggagtg gggtattttg atacgattga aataaaaaca atattgtcat
    11401 tacttcaaat aatagataat ccaatgcaag atatacctct tatatctgta ttaaaatcac
    11461 ctatatttgg atttacacca gaggatttga ttgatattag agttcagagt aaagataaaa
    11521 ttttttatga ggttttaaaa agtacagcag agtatgatgg atttacagat tctcaaaatg
    11581 aaaatgagtc tgagtttata ccaagtgaag agtgcataaa caaaagtaaa gattttttaa
    11641 ttaagctaaa ggaatttaaa gaaaagtcaa tgtacatgag tacagatgaa tttatatggt
    11701 acttgtacac aaggactgga tactatgcct atgttggagc attgccaggt ggaagtcaaa
    11761 gacaagctaa tttaaaagta ttgtttgaga gagcaaaaca atttgaagaa accagtctta
    11821 aaggaatatt caactttgta aattttatag agaaattaaa aaaatctagt tctgatatgg
    11881 gaagtgcaaa aacacttggt gagaatgcaa atgttgttag aataatgagt attcataaaa
    11941 gtaaaggtct agaatttcca gtagtcatat gctctgcaat gggtaaaaat ttcaatactc
    12001 aagattttaa aaagagtatt ttatatcatc ataatttagg gtatggacct caatttgtag
    12061 actatgagag aaggatttct tttccaagta tagccaaaga agcattaaaa agcaaaataa
    12121 atatagaaaa tttatcagaa gagatgagag ttttatatgt tgcatttaca agagctaaag
    12181 aaaaattgat tataactggt tctactagaa atatacaaga tagtataaag agatggtcaa
    12241 atggaattga aagtttagat acaatatcac aatacgagat attaaaaggt aaaaattttt
    12301 tagattggat aatgccttgt gtattaagac atagggattt atctaatcta ctagaagaag
    12361 taggattaga cgcagttttt aatgttgaac ataattcaaa atggtatgga aagttgtgga
    12421 ataagaatga tattttagtg gaaaagaaga gtgatgaaga aaaagaaagt attgaagaaa
    12481 ttcttgaaaa aatagatgta gataatcctg acagtgatta ttacagtgaa atagaagaaa
    12541 aattaaacta tatatatcca tatgaattta gtacaagaaa acctgctact atatctgtta
    12601 cagaaataaa aaagatacaa aataattatg aagaagagtt aataaatact atatttgaac
    12661 aaaaagtaat attaaaaaag cctctattta ttcaaaatga agaagaaaga gaaaaaataa
    12721 gtggaacaga aagaggtaca atagtccatt tagttatgga ggttttagac ttaaaaaatg
    12781 taagtagtgt aaatgatata aaatcacaaa taagaggttt tgtttcaaaa ggtataataa
    12841 cagaaaaaca agctagtata gtaaatccat ataaaatata taaatttttt gcctctaaca
    12901 taggaaaaag aatgttgaat gctgaaatta taaatagaga aaaatcaatt tatgcacaag
    12961 taaatatgaa agacatatat atttatgaaa agcttataaa caatgacgat aaaaaacttt
    13021 atgataatga aagtgtcatg ttaagaggta tagttgatgc ttattttgaa gaagataatc
    13081 aaatagtttt ggtggattat aaaacagatt ttgtaaatga ggaaaatata aatcaaataa
    13141 tagagaaata taaaaaacag ttggatttat atgcagatat tattgaaact ttaactggta
    13201 agtcagtaaa agagaaatgt atatatttat ttggagttga tgaagctgtt tgttattagt
    13261 ttttatatat aaattaaacc taaaagatag aggaagggga aaaaatgaga tttattcata
    13321 catcagattg gcatctaggg aagtccctag aaggccattc tagaattgag gaacaagcaa
    13381 agttttgtga ggaatttata aaaatagtag aatcaaatga aatagatatg gttataattg
    13441 ctggagatgt atatgatact tctaatccac cagcacaagc agaaaaacta ttttatcaga
    13501 ctgtatcaag acttgccaat aatggtcaga ggtgtgtatt aataatttca ggaaatcatg
    13561 ataatccaga gagactctct gcaataacac ctctagcaca tgaacagggg atacttatat
    13621 atggttatcc attaagtgct acgatagaag ctaagtataa agggtttgaa ataacatatg
    13681 caactcaggg atgtacaaaa ctgaatataa atggagaaaa tattgtggtt gctacattac
    13741 cttatccaag tgaaaaaaga ttaaatgaag ttttttcaag tgaagatgag tttgagaaac
    13801 aaaaaaatta ttctgaaaag gtaggagata tatttagaag tttagaagaa aattttagaa
    13861 gtgatactat aaatatagcc gtatcacata tatttgtaat aggtggagag agtactgaat
    13921 cagaaagacc tatacaatta ggtggaagtt ttttagttga aagaaaagat ttgccagaaa
    13981 aggctcaata tactgcacta ggtcatttac ataaacaaca aaaagcttcc gaacgtttaa
    14041 atgcgtacta ttctggttct ccacttcaat atagcaagga tgaaagagcg tataccaaag
    14101 gggcatacat agtggatata aaagctggtg aaaaaccaat aattgaagat gtatatttta
    14161 ataattacaa acctatagaa gtatttaaat gtaatggaat agaagaagca ctagatatat
    14221 gtgagcaaaa tcaagataga gagatttggt catattttga aataaatact gatgagataa
    14281 tatcacagaa tgaaattaaa aaaatgaagg aactattaaa agacataata gaaataaaac
    14341 caataataac atcttgttat gaacaagaaa gtgtagatat aaaagaaaag tctatggcag
    14401 agttgtttag agagttctat tcttttagta aaggtgtaga gccaaaagga gaacttatgg
    14461 atttatttct agatataata agtgaagaag gtgaatcagc agatgagacc aattagatta
    14521 gaacttacag ggcttaatag ctatattgat aaacaagtta tagactttga aaaattaatt
    14581 gaaagaggtt tgtttgggat atttggaact actggaagtg gaaaatctac aatattagat
    14641 gcaataacta ttgctatgta tggaaatata tccagaaata caaaagaata tataaacagt
    14701 gtgtgcgata aggcaattat atcatatgag tttgagatag gaagcaaaaa cactagaaga
    14761 agatacattg tagatagaac cattgctaga agcaaaacag gaacaaagac ctctaatgct
    14821 agattaatag aagtattgaa tgacaatacc caaaatgttt tagctgataa agttgtggaa
    14881 gtaaatgaaa aagtagcaca ggttgtagga ttaacggcaa atgattttac taggtcagta
    14941 gttttaccac aagacaaatt taatgagttt ctaagattat cacgtgcaga tagaagagac
    15001 atgttagaga gaatttttaa tcttgagaaa tatggaagaa gtcttggaga aaaagtgaaa
    15061 aagagaaaaa atatacagtt acaaaattta aaagatttga aatcaaaatt aagtcaatat
    15121 gatggaatta cagaggaagt atataacaat ataaaccaag aattaataga gttaaaaaat
    15181 ttggaaaaag ataagaataa tgctttagac ctagctcaaa aatcttatga agaaagtaaa
    15241 acagtatatg aagaacaatt aaagttagaa aaaaatgaac ttagaaaaaa tgagcttgat
    15301 ttaaaaaata gtgagataaa agaaaagatt gctcttgtag aaaatgatag taacgctaaa
    15361 aaggtagacc catacataag ttcagttcaa aatctagaaa aaaagataga tgaagatagc
    15421 ttcgcagtaa gtaatttaga aaagaaatta gcaatattaa atcaggagct tgaagttaca
    15481 aaaaatagat atgaaaaaat aaacaaaatt aagaatgaag aagtccctaa attgagtgaa
    15541 gaaaaaataa gattgcaaca agcaataaaa ttagaagaag agttagtttt attagataga
    15601 gagttaaaag agctaaaaga aagtggaatt aatttaaata agactaaagt tgaattagaa
    15661 aaagtaaagc aggtgtcaga gtccagaaaa gatgcagtta ctaaatctat aaaagaagtt
    15721 gaaggaaaga ttgataaagt aaatataagt gcagaattaa aacaaaaaat atttctagca
    15781 tatgaatacg agaaagatta caacaacgta ttggaagaaa aaaatcaaaa gctaaataaa
    15841 ctagaagaaa tactcaaaga gacagaaaat ataaatttaa aagttagata tattgataaa
    15901 gataagaatg atgtaaatcg taatttagaa aatttatcac ttcacttgga tgttcttctt
    15961 aaaaaatgtc ctggtaagag tgctgattta cttttaaaat ctgaatatgt tactgaatta
    16021 agaaataaag caaataatac taaagaaaat gaaattaaga aaagcagtat acaagatgaa
    16081 ttaaagataa tacttgaaag taagtttaat actgaaaggg aagtaaattt actaaatgaa
    16141 aaacttgaaa ataatagaaa gaacagagat gacctagaaa aagaattaga agaactaaag
    16201 tatttaaatt tagcctctga acttagacgt gaattaaaag aaaatatgcc ttgtccagtt
    16261 tgtgggtcaa aacatcatga aaatcataat ataactaaat atgatgaaaa tatatctttt
    16321 gtcaaagaaa aactggagaa attagaaaaa gaaaaaataa gtataagaaa caatatagag
    16381 gaattaaatg ctaaagtaag tgggtattta tctatagaaa aaatgaagac taaagaactt
    16441 gaagatgtaa aaggaaaact aggggggatt ccatctagtc aattattaaa aaaacttgat
    16501 gaagaacaaa gaaaacttgc attgttaaaa tctaatattc aggagtggga gaaagaaaaa
    16561 gagagtactg aaaataaagt aacattagcg aaagaagaaa aaaataaaat tgaaaaagaa
    16621 gaattaaaaa taagagagag tctgaataat tataagaaat taacaagaga tttaaacata
    16681 gaaatagaag gtttagaaaa taaatgtaaa aaacttaaac aggaatacct aggattaaag
    16741 acgattacta aagtctctaa tctattgtca aaagtaaaag aaataaggga gaatgaaaaa
    16801 gaattagaat tactaagtac tgggcattca aatttattaa aaaatagaga ctcactagat
    16861 attaaaataa gagaaaatga gagtaaacta catgaaatag agatagaatt aataaaggct
    16921 agagaattat atgtagaaaa gaaattgagt agggataata agtataaaga agttattagc
    16981 ataactaaag gtgatttagc taaaaatctt ttacataatg ttgaagaaaa tatatgtaag
    17041 atattagaac aagaagaatc aagcaagaaa aaattggaag agcaaagatt agaatatgaa
    17101 aaaaacattg ctgaaaagca caatatagat ggtagactta aaactgctaa ggaacaatat
    17161 aaagaccaaa aatcaacact aaataaacta ttagcagaaa ataaatttga aagtatatat
    17221 gcagtaaaaa gagctttatt agatactgat gcagtaaaaa aattgattga agaaatacta
    17281 gagcatgaag aagaacaaaa gttattatca tttaaaataa aatcatcaaa agaaaaatta
    17341 aatggaagaa gcataaagaa ggagtacttt gaacaattaa aagatgaaat ttataatttg
    17401 aaagtagaaa taggaaatat atccaaagat attggagcta atcaaaatca gttaataaca
    17461 ttaaaagact ctttagataa gataaatgat tttaataaac aacttaaggt agttgaacac
    17521 aatgtggact tattggaaga attagataaa tgtgtacaag gaaataaatt tgtagaatat
    17581 gtttctacaa accaattaaa atatatagcg ttggaagcat caaaacgatt ggatggaata
    17641 acaaaaggaa gatatgcact tgaaattgac tcaacactaa actttgtaat gagagataac
    17701 tttaatggag gagaaagaag aagcattgat actctatctg gtggagagac attcttgaca
    17761 tctctatcat tagcacttgc tctttcatct caaatacaat taaagggaag tgcaccatta
    17821 gaatttttct ttttagatga aggttttggt tcattagata gtgaactttt agaaattgtt
    17881 atagaatcct tggaaagatt gcatagtaat aatctaagtg tgggtataat aagtcatgtc
    17941 gaggaactta aaaatagagt tcctgttaag ctattagttt catctagtga ggctggaata
    18001 ggttcaaaag taaaaattga atatagttag aaaatatatg ttaattgaat atattatcta
    18061 atatttccaa ataatatgta attgtgatat aataaaaact aatattaaag ttatttataa
    18121 ttaatattaa tataaaaaca tgtaagtagg agtgtggtaa gtgttggaat cgcccaataa
    18181 tttgattcag attatttttt taatagtatt acttataggg tctgcatttt tctcagcatc
    18241 tgaaacggcc ttaatgtcgt tgagtaaaat cagaatcaga tatatgcaag atgaaggagt
    18301 taaaggagct aagttagtaa gttcattaat agaaaatcca aataagcttt taagttctat
    18361 attggttgga aataacgtcg taaatatagc agcaacatcc atatcaacat ctttgtttat
    18421 aggattaatg ggagaaaaag gagtggcact tgctacagct gttatgacag tattagtatt
    18481 aatatttggt gagattactc caaaaacaat agcagcaaat aattcagaaa aggtttctct
    18541 attagtatct aaacctataa aagcaattat atttatatta agaccaatag tatggatatt
    18601 taatataatt acaaatatta tatttaaatt atttggtata acaaataaag gagctaaatc
    18661 attcataact gaagaagagc taaaaactat ggtaaatgta agtcatgaag aaggcgtcct
    18721 tgaaatggaa gagagagaaa tcataaataa tgtttttgaa tttggagata tgcaagctaa
    18781 aaatgcaatg gtacaaagaa tagatatggt agctatagat atggaagata gttatgatga
    18841 gataatacag gtatttaaaa ctgaaaaatt aagcagaatg cctgtgtatg aagaaactat
    18901 agatgatatt gtaggaatac ttaatataaa agatattata tttttatctg atgaagaaat
    18961 agagtcattt gatattaaaa attatatgag agaacctttt ttcacgtatg agttcaaaaa
    19021 gataacacaa cttcttgaag agatgaaact tgaaaaaagt cagatggcta tagttgtaga
    19081 tgagtatggt ggaacatctg gacttttaac tatagaagat ttagtagaag tcattgttgg
    19141 agatatagag gatgaatatg atgaagaaga ggatgaaata caagttataa aagaagatga
    19201 gtatattgta gatggaagta ctaaaattgg tgatgttaat gaacttattg gtgtaaactt
    19261 ggaatctgag gagtttgatt ccataggagg ttttataatt ggacacctaa gcagattgcc
    19321 agaagaaaat gaagtaattg aagttgataa tataagattt tgtatagaaa gtatagaaaa
    19381 aaatagaatt aaaaaaataa gaatatatac ataatgtaat aatgttctta aatttattag
    19441 aagtgtataa atataaaaga tatcttttat atttatacac ttctttttat tttgtattca
    19501 aataaaacta gtatgaaatg gacaaaataa aaatataaat tagaagaaag gagattttta
    19561 agatgagcat actagaaaat aattttttat acaaattaaa aaacaacact atcttttttt
    19621 gtatatatac attgttattt atttttattt acaaagcgtt tccatacata gctccatttt
    19681 ttctaggtgc tataatagca cttatgatta atcctatatc acaaaagtta gaaaataagt
    19741 ttcatataaa taaaggtatc tctactcttg ttctaagttt tcttgcagtg gctatagtca
    19801 gtacagttac tacaataata gtaataaatt caatgaaaga attaatggga tttttaaata
    19861 atatatctgc taatcctgag gatataagta atactattat gtatttttta aataaaataa
    19921 atgactttat gaaatcattt caagaaatag ccaattttga tgtagaacaa ttagtaaaca
    19981 aattcagtgg tgaagtaatg caaattacaa aaaatcttct tactagtata cttggattgg
    20041 ctacttctat tccatatatt ataatattta taattacctt gtttattgct acgtacttta
    20101 tagcaaaaga ccttgataag attgaaaata gtttttataa tatgtttact gttgatgtta
    20161 gaaagaaagt aaaaaacgta aagaaagagg cagggctttc acttgttgga tatataaggg
    20221 cctatacaat acttatggct ataacatttt ttgcaatctg gggaagtttt gctttatttg
    20281 gattgaaata cggtctaata gtagggtttg taggagcttt aatggattta attccattcc
    20341 taggtataat aacaatatat cttccagtaa ttgtttatta ttttcttata aaaaattatt
    20401 ttatagctat aagtatgact gtgatatttt ttgttttgtc tttgattaga gaaatacttg
    20461 aacctaaact agtatcagta aatgttggac taaatccatt agctacccta gcagcgattt
    20521 ttattggaat ccaagtaaaa ggaataattg gagtaatttt ttgtttgggt ttggtatgta
    20581 tgcatgatat acttaagaag gtggatattt tttaattata ataattgata gattttttta
    20641 aaaataaaat tttattcaca ggtaaataaa aaaatattaa tcttattaag aaagagggta
    20701 tatacgtgaa acaaaaagta gtggaaagat ttttaaaata tgtatcattt gatacaacat
    20761 ctaattctca gtgtgagaac tgtccatctt cagaaggtca aagagtttta gctaagtata
    20821 tagttgaaga gctaaaaaca atgggtgttg atgatgtgag tttagatgaa aatagttata
    20881 taatggctac tttaaaagga aatactgatg gtgtggatac tataggattc atatcacatt
    20941 tagatacaat tgaagatgtt agtggaaaag atattaaacc aagaataata gaaaattatg
    21001 atggaaaaga catagtttta aatgaagcgt taaatgttat tacgtatgta aaggattctc
    21061 cagagttgga agaatttaaa ggtgacgatt taatagtcac tgatggaaca actctacttg
    21121 gctctgatga taaagctggt atagctgaga tagtgactgc aatagagtat ctaataaatc
    21181 atccagaaat taagcatgga gatataaaaa taggatttac accagatgaa gaaattggta
    21241 gaggtgctga tttatttgat gttgaaaaat ttggagcaaa atatgcatat actttagatg
    21301 gaggaatagt tggagaactt gaatgtgaaa attttaatgc tgcaaatgca actattacta
    21361 tacatggtag aaatgttcat ccaggttcag ctaaaaataa aatggtaaat gctatacata
    21421 tagctgctga aatatcagaa atgttcccag cagatgaaag accagaaaca acagaaggat
    21481 atgaaggatt ttggcattta aattctatag gtggaaatgt cgagaatgta agtatggctt
    21541 atattattag agaccattgt agagaaaaat ttgaaaatag aaaatctatt atgatagaga
    21601 atatagaaaa aataaataag aaatacgata atagagtgga attggattta aaagattctt
    21661 attacaatat gaaagaaaag attgagccag ttatgtttat agttgatata gctaaagaag
    21721 ctatggagga attaggtata aaaccacgtt tagttcctgt aagaggtggt actgatggag
    21781 caagattatc ttttaatgga ttaccttgtc caaatatatt tactggtgga ttaaacttcc
    21841 atgggaaaaa tgagtgtata ccagttagtt ctatggagaa agctactaaa ctaattgtaa
    21901 gaatagctga aaaatatgct gaaagagttt agttaaaagg tatttaaaaa ttaagcattt
    21961 aaagttataa tagctatatc attctttata agtcaagctg attataagct tgtgttgtat
    22021 ttaaaatggt atagctattt ttgtaattat ttttataaaa gataattatt tttagtttag
    22081 ggtatcaata aaaagtatga atatcaatag gtattaacat tcaaaagggg gaattgtatt
    22141 gaaaattaaa tctaaaaaaa taatagcatt tgcactttca cttgggatta tgttgccaag
    22201 ttgtgtatat gcagacacta ttagtaaaaa tcaaaatgca caggtagata tgaacaatta
    22261 tcttcaaggt agaaaatctc caaaggagga aaaacttatt ggtgaagata gatttgatac
    22321 agcgataaaa atatctcata gtggttggaa taatggttca gaaagggtgt ttctagttaa
    22381 ctcaaattca ttgccagatg cattggcttc aactcctttg gcaagtaaat tggatgcgcc
    22441 aatactgctt acaaataaaa atagtatacc atataatgta acagaggaaa taaaaaggct
    22501 taatccagca gaagttattt taattggaag tgaaggagct atttcatcaa gtgtaaaaga
    22561 atctcttgaa gatatggaag tattagtcag tagaatcggt ggagcagata gagaagaaac
    22621 atctttactt cttactagac agctagatga tactggtgat ttaggtgtct caaaagtggc
    22681 agtggttaat gggtataatg gactggcaga tgcaacttca atatcaagcc cagctgcaag
    22741 tgataataca gctataatat atacaggtaa ggatagcata agaagtgagg caaagaattt
    22801 tattacacaa aactcaacta agacatacat agtaggtggg gagtacagta taagtaaaaa
    22861 acttgaagga cagttagtaa actcagaaag actagcaggg acagatagaa aagatactaa
    22921 tgcgaaagtt ttagaaaagt tttatggaaa atcaagtaag gttaataata tgtattttgc
    22981 aaaagatgga agtggtagag aagctgattt agttgatggg cttgctgctg gagtattagc
    23041 atcaaaaaca aagtcacctg tagtacttgc aagtggaagt ttaagtagtg cacagaaaag
    23101 ttttataaaa aaagtaaagg ctgataaatt tgtacaagtt ggtggaggta aaaatagtaa
    23161 accatatgca gaagcattag ccttacagta atattgtgta aatcaaaatt aatatataat
    23221 aatagcaagt aaaccaagtt gcacttcaat atatataata aaataagtaa actaagttat
    23281 atttcagtat aatatataat aa
//
LOCUS       DAKBNN010000043        23013 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000043 DAKBNN010000000
VERSION     DAKBNN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.043"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(220..1590)
                     /locus_tag="OUB02_001448"
     CDS             complement(220..1590)
                     /locus_tag="OUB02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HCT8885214.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            1873..1971
                     /locus_tag="OUB02_001449"
                     /pseudo
     CDS             1873..1971
                     /locus_tag="OUB02_001449"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            1990..2411
                     /locus_tag="OUB02_001450"
                     /pseudo
     CDS             1990..2411
                     /locus_tag="OUB02_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2642..2914
                     /locus_tag="OUB02_001451"
     CDS             2642..2914
                     /locus_tag="OUB02_001451"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8885215.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(3277..3357)
                     /locus_tag="OUB02_001452"
                     /pseudo
     CDS             complement(3277..3357)
                     /locus_tag="OUB02_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(3397..4065)
                     /locus_tag="OUB02_001453"
     CDS             complement(3397..4065)
                     /locus_tag="OUB02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCT8885216.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(4901..7321)
                     /locus_tag="OUB02_001454"
     CDS             complement(4901..7321)
                     /locus_tag="OUB02_001454"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCT8885217.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVETFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7675..8025)
                     /gene="rsfS"
                     /locus_tag="OUB02_001455"
     CDS             complement(7675..8025)
                     /gene="rsfS"
                     /locus_tag="OUB02_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCT8885218.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(8142..8714)
                     /gene="yqeK"
                     /locus_tag="OUB02_001456"
     CDS             complement(8142..8714)
                     /gene="yqeK"
                     /locus_tag="OUB02_001456"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCT8885219.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8715..9404)
                     /locus_tag="OUB02_001457"
     CDS             complement(8715..9404)
                     /locus_tag="OUB02_001457"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCT8885220.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            9680..10318
                     /gene="recX"
                     /locus_tag="OUB02_001458"
     CDS             9680..10318
                     /gene="recX"
                     /locus_tag="OUB02_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCT8885221.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            10471..12474
                     /locus_tag="OUB02_001459"
     CDS             10471..12474
                     /locus_tag="OUB02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCT8885222.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFNSTPPSSKMPIASRTAIVLLDNGI"
     gene            12648..13289
                     /locus_tag="OUB02_001460"
     CDS             12648..13289
                     /locus_tag="OUB02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8885223.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            13382..14065
                     /locus_tag="OUB02_001461"
     CDS             13382..14065
                     /locus_tag="OUB02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885224.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIHNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(14242..14553)
                     /locus_tag="OUB02_001462"
     CDS             complement(14242..14553)
                     /locus_tag="OUB02_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCT8885225.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(14631..15449)
                     /locus_tag="OUB02_001463"
     CDS             complement(14631..15449)
                     /locus_tag="OUB02_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8885226.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEVVSQLFAKKLEESLSPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            complement(15549..16073)
                     /locus_tag="OUB02_001464"
     CDS             complement(15549..16073)
                     /locus_tag="OUB02_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT8885227.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIIFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKNCIK"
     gene            complement(16092..17234)
                     /locus_tag="OUB02_001465"
     CDS             complement(16092..17234)
                     /locus_tag="OUB02_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCT8885228.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            complement(17514..19901)
                     /locus_tag="OUB02_001466"
     CDS             complement(17514..19901)
                     /locus_tag="OUB02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8885229.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            complement(19894..20577)
                     /locus_tag="OUB02_001467"
     CDS             complement(19894..20577)
                     /locus_tag="OUB02_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885230.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            complement(20694..22004)
                     /locus_tag="OUB02_001468"
     CDS             complement(20694..22004)
                     /locus_tag="OUB02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885231.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            complement(21998..22705)
                     /locus_tag="OUB02_001469"
     CDS             complement(21998..22705)
                     /locus_tag="OUB02_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885232.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
BASE COUNT         7807 a         3778 c         2671 g         8757 t
ORIGIN      
        1 tacattataa taatattctt ttaatcaaaa aaacacctac tattaaagta aatgttttta
       61 agtattttta attttaaaat ccatatagtt catatgaaac taaatataat aactcaacat
      121 atgagcaata tcgagacatt tacataccac atagttaata taaaacgaaa ataaaaaaat
      181 taaacttata aaacccttat taatttacat acccactagt tatttatatt caaaaaaatc
      241 ttctccatct gggttataaa ataatattct acttgccaat ccttgttcat atttataatt
      301 ttctttatca cctacataag aaaatgttaa tgtcatgctt cttgatggat ctaatgaaat
      361 tatatctaaa taaatagtac atttttctcc tggctttaat acaccgaaac tattaaaatc
      421 catttttatt ttagcatatt gccccatata taatgttgaa ttagcttctc cataaacttt
      481 taattttgtt acatctaaat tttgtgtact tgtattttct atatttatag gaactgttgg
      541 gtctttatat ttttcagtat aatattcaag gttatcatca taacattcaa gataacttcc
      601 tttatcttgc caaattatgt gctttgaatc aaaatcaatc tttaatccac atattatatt
      661 tttatttgaa gacggaagac taatattatt gatggtattt tcacactcta gcataatttt
      721 atcacttaga tttcctatcc cagtcacttt tgcagcattt ttaagtatat ctttattact
      781 tccaatatca actagtacaa taggattatt tttagccatt ggtgaagcaa ctaaagcata
      841 aactaaatca gtaccactag ctatataaaa ttcttttgcg ccattataga acttatttac
      901 tattttttta ttagtatcat atctatctac tccacctaat ctagttgaat tagtatcatt
      961 aactagtttg tcactcattg atgaagtacc acctattaca taactttcta caccagttgt
     1021 attaaatgga acactttttc catcagttaa tactatagct gctttatctc taactgctac
     1081 aggtgcagca ctcattgcat caggctctcc tttaaaagca ttagtcaata taactttatt
     1141 tactttgtta attgagttta tttcttttgc aacattatag cttgtcttaa ttctatcact
     1201 accttgaagt cttttagcct ctattccttt atcttttaga agagtttctg ttgctttatc
     1261 aatagaactt tctccaccta ttatgtatac ctttttagct ttctctactc ttttaagagt
     1321 tgcattaggt atgttatttt tcttagttaa aaaaattggt gcatttgttg ctcctgcaag
     1381 tccactcgca cttaatccat cagccattgt gctgtctgca ttaattaata tagctgtagt
     1441 ataattttgt tcatcagcta taagagcagc cgtttcatat ttatcttttc cttgaatagt
     1501 ataaattgta tcaagtgcat tagctgatat aggaccagca gcaaacattg acacggctaa
     1561 ccctagtgat aataatttct ttaatttcac aatatctccc ccttttatat atgaaatact
     1621 atatatctta cataattcta cattaaattt tcttttcctc ctttaatgat atttatcttc
     1681 ctgttaattt aagtgtatac aaatacgtgt tataatgtaa ctgtaaagaa ttaaaaagat
     1741 aatgacataa ttagagcagt ccaactgttc ttttataaaa ctaacttata aagggagcgt
     1801 acatatatga atatcaaatc agcttttata agaaaaagat gggaaaaatt ctatgtatat
     1861 gtggaataag aaactggcaa aaagaaacaa aaaagttatg gaagctataa aaagaaaaag
     1921 gatgctgaaa aacatttaat tgaaataaag tctactataa ataataataa gtttgttgct
     1981 ccaaaaggta tgacatttgt agaaagatgt tataaatata tagacatgaa taaaaataat
     2041 tggtctccat atactgttac aaatacaaaa tcttgggtta aaaattacat agaacctttt
     2101 ttaagaatct aaaactcaca gaagtaaatc ctagttcagt acaagctttt gtaaattata
     2161 actttaataa ttctaccttt actagtgcta aagtaaggta tagctttcta agttctgtaa
     2221 taagggaagc gtatagatta agagaaatac aagaaaattc atgtgatttt ataaaacttc
     2281 cttctaaaga aaaatattct acaaatgaaa tatacaatag agaagaagta ttattgctaa
     2341 tagaaaagtt acttggtaat aatatagaaa tacctattct tttaatgtta cttctaggtt
     2401 taagaatgtg agaagctact gggcttaggt ggtcagatgt tgacttagat aacaatctga
     2461 taaatgttaa tcaaattctt gtctattcta atggcaaaat atcttttaaa gagccaaaaa
     2521 caccaaaatc aaaaagagct ttatcagctc caaaagaatt aattgaaaaa ttgaaaattg
     2581 aaaaactaag acaaagcaaa ttaaaattgt aaggtatcct ggaaaacaga aataacttag
     2641 tttgtctaaa caccaattta aatccgtttc taccttcttc actaagaaaa acgtttcaca
     2701 attttacaaa aaaaataatt taaaaaagat tagaatacat gatttaagat atacaagtgc
     2761 cactctgttg cttttagggg gaactaatat gaaagttgtt tctgaaagat tgggtcatac
     2821 agatataaaa ataactatga atagatattc tcatgtactt aaagaaatgg acaaagaagc
     2881 ttccgacaat ctaagcgaac ttctatttaa atagtaaaaa ctgactgtca gttaaatgtc
     2941 agttaaacct aaaaaaatag gttttgtcag ttgattatca gtcaattatt ttataatagt
     3001 ttcacttaaa aacgtctata tctacacatt ttactatttg tttatgctta ttaatatcta
     3061 tctgttatta ttaattctaa tagcttctat ttaattattt agttcagttt acaggaaact
     3121 acacacaaac cataaaatag gatgttgaca atataacaga tagtatgctc ctgtaaaagg
     3181 taacatacta tctgttatgt tgtctggatt tccaaatggt taccctttgg cacacgactt
     3241 tgctatctcc ttggtaaact aaaagtttgt aatctaaaat cctaatccat ctctataaaa
     3301 tttaattgac ttctccatac tttttacacc taaacatata aaagtaattt tattcactta
     3361 atgacctcca taattcccaa attagctatt tggaacttaa ctaaaaaatc ctaatataag
     3421 ggcaactatt ataatggcaa tcggaataca tactgtgtaa tcctgttcca tagagtcatc
     3481 atctgaatgt agttttacaa ttcgcaattt agcaatcagt ttgaagcaaa aatagcctat
     3541 tattgttcca attacgttcg tgagtaagtc gtctatatcg gttgctctgt gtaacgtaaa
     3601 aagctgaata gtttcgataa aaagagataa tccaaaacca atcaagaatg tatttctcaa
     3661 acgttcaaag gttttgctta taagcggaca taaaaaacct aatgggacaa aaagtaaaat
     3721 attcaaaata aaactcaaac taaatccatc actaaaagga ataagattta tgtttggatt
     3781 aaagaacgtt tcccctaact gtgacagtct tatatattca ccgaatgtag gtataccaac
     3841 aatatgtgat agtgttaaac ataagtagta atacattgcc agtgataaaa tagatgtttt
     3901 tacctttgga atatcactat ttttcttgaa tattacaact aagaaaatac caatcaagat
     3961 aattcccaac aagaaaatag gattaattaa caataaatct ttgatagctt caaacagact
     4021 gagttgaatt tctacacctg attgattatt tgttacagtc tccatttgta catacctcct
     4081 cataacattt caattatttc taaaaaataa aatagaagat aaatcttaaa atcatcctat
     4141 caaaattttt aaatttgcct ttatcagttt ataccaccag ttaattcctg tcttacttct
     4201 gtatggtatt cttgggattc tttcatcaaa ttagacaaaa aatggttacc atatcaataa
     4261 actggttacg ctccatatct cccgaagcaa ccatcccctg agaataaatc cgtatcagga
     4321 atctccacat ctactgcacc tatcacaagt tatttgataa catcctcaga caatcccaac
     4381 ttggatatat cgacaacacc cattagaata ccactgctga ccatacttgt tttcctgctg
     4441 ataattttaa tcaactggga agttgaaacg ctatcttttt tacaagttcc tttttagaaa
     4501 catggtagct attatatgaa tctgaatttc gttgtctggg tgtttcatat aaagcataga
     4561 tatgcacctt ttttgcctgt tttcattata tagaaaaaaa tttcaaaata caataattat
     4621 atttttttcg tttaagctta gagcagttcc ttgatgaaca acactttgga aactgaatga
     4681 tttacatacc acttaataca ttaactcgat ttaacattaa ttataataat atttaaaaca
     4741 acaactaggg tatataagaa tgaacttttt agttctttaa tcattttttt gtataaaatc
     4801 aaatctattt ttataatttc aacaatgaaa aaaggcttat ctctattttg agacagcctc
     4861 atttatttta taaatttata tttttgttca tatttttgag ttatttaaca actatattta
     4921 ctaatttttt aggtacagct acaactttaa ctatagtttt accttcaact aatcctttta
     4981 ttttttcatc ttctaaagct accttttcca tctcatcttt agatatattt gagttaacag
     5041 ttaattttcc tctaactttt ccattaactt gtacaacaac ttctatttca tcttttacta
     5101 aagctttttc gtcatattta ggccagtcta tatcaaatac acttccttct ttacctatca
     5161 tagtccatag ttcttcacct aaatgagggg caaatggtgc aattatagtt actatagttt
     5221 gtacaccttc ttttataact gcttcgttta tattatctaa ctctttatat ttatacatat
     5281 catttattaa ttccataaga gctgatatag cagtattaaa tccgaatttt tcacttaaat
     5341 cagcagtaac ttttttaagt gtagagtgta tagtatatct catatcctta tcttgggaat
     5401 ttaattctcc aaactctaca tcctttttaa ccacatctgc taactcgtca accaatctat
     5461 aaaccctatt taagaatcta aagcatccat caacaccttg ctctgaccag tccaaatctc
     5521 tttctggtgg tgctgcaaat aatacgaaaa gtcttgcagt atctgctcca tactcatcta
     5581 ttatatccat aggagatact gtattccctt tagacttact cattttagaa ccatccataa
     5641 gtaccattcc ttgagtaagt aagtttttaa atggctcatt gaagtctacc attcccatac
     5701 tcttaaatgc ctttacaaac catcttgcat aaagtaaatg cattatagca tgttctactc
     5761 cacctatata ttggtctact ggatgccatc tattaactaa ttccttacta aatggttcat
     5821 tttcattatt gctatccaca tatcttaaga aataccaaga agaatcaacg aatgtatcca
     5881 tagtatcaac ttcccttcta gctggttttc cacagtgagg acaagttgtt gacataaatt
     5941 gctttgaagt cgtaagtgga gattcccctt ttccagtaaa ttcaacatct gttggtaaaa
     6001 gaactggtaa atcttccttt ttaactggaa ctattccaca ttcatcacaa taaaccacag
     6061 gtataggaca accccagtat ctttgtcttg aaactaacca gtctctaagt ctataattaa
     6121 ttgttctctt tcctctacct tctttttcta acttatcgat tattccttca aatccttctg
     6181 aagcatctaa tccattaaat tcttctgaat ttatcatttt gttatcttct gttattacat
     6241 caattatgtc taaattatat ttttcagcaa aatctctatc tctttcatca tgtgctggaa
     6301 ctgccattat agcacccgtt ccatactcaa ctaatacata gttagctatc catagaggaa
     6361 ctttcttgcc atttaaagga tttataacat atctacctat aaacattcct tctttttcta
     6421 catcagctgc tgttctttct atttctgtca tagtatgcat tttttgaaca aaagtttcaa
     6481 catctgcttc atattcagtc cctgcaacca attctttaac taattcatgc tctggagcaa
     6541 gtaccatata agtaactcca taagttgtgt ctggtctagt tgtaaatact gtcatagatt
     6601 tatcagttcc atctatatca aaaactaaat ctgctcctgt acttttacct atccaatttt
     6661 tttgcattgt tttaactttt tctggccatc catctaatgt atctaagtca ttaagtaatt
     6721 cttctgcaaa ttcagtagtc ttaaaatacc attgttctaa atctttcttt aatactgttg
     6781 ctttacatct ctcacatgct ccttgaacaa cttgctcatt tgcaagaaca gtctcacaag
     6841 aaggacacca gttaacatat gattttttct tatatgcaag cccatgctct aaaaacttca
     6901 aaaatatttc ttgtgtaaat ctatagtatt ctggagttga agttgcaact tctttatccc
     6961 aatcataact aatccctaat aaatttaatt gctctttcat ttcttctata ttttccattg
     7021 tccatttatg cggatgtatt ccatgtttta tagctgcatt ttcagctggt aatccaaaag
     7081 aatcccatcc cattggatga agtacattat atccttccat ttttttgaat cttgcgacaa
     7141 catcacctat agaataattt ctaacatgtc ccatgtgtat ttttccagat ggataaggga
     7201 acatttctaa tgtgtaataa ttaggttttt cagtttgtgc agtatccatt ttatactgat
     7261 tattatcttt ccagattttt tgccatttcg attctacttc tttaaaatta tatacgctca
     7321 tattttgcct cctattttta attgtgtata actaccttat ttagtattat ttacttataa
     7381 tttccaaata attctttgaa aataaaaaat cctcgtcact aaacttacgt ttagggacga
     7441 ggataaatct tcgcggtacc accctaatta gcctactaca tatatagact ctcttagata
     7501 gcttttaatc cagccatgga gaatagattt taatctagta agttcaaagt tatataatat
     7561 tagttctcac cttccactaa ctctctgaaa aaatataatc ttttactgct ctagtctatt
     7621 caatttaata taatttttga aattttctaa ttataataac atatattatc atgtttatgc
     7681 taatgtgtca atatctatat aaggtgcatc tttccataat ttttctaaat tataaaaccc
     7741 tctattttcc tcattaaata catgaaccat tatgtctcca taatcaagga gtacccaagt
     7801 ttgagttcct tgtccttctt tacctcttgg ctctaatcct agttttgcta attcatcttc
     7861 cacattgtca gcaatagctt ttacctgtct ctgagaagat gctgtagtaa taataaaata
     7921 atcacacaaa ctagaaactt ttcctatatt tattataact gtatcttgac ccagtttatc
     7981 ttctatggca tcatatgcta tttttgtcat ttgttccact gtcatataaa aattcctcac
     8041 tttcctaatt aaaatacact tagttattgt aacatattaa tacgattttg taacatagtc
     8101 aaatttaaat ttattaatat gtttgccttt atatagcttt tttataataa cttttcttta
     8161 attagatagt ttcttgccat aactgttctt ggatgtatct cttctttttt attgatgaca
     8221 aacattaaag tattattaaa agaagttaaa agtgcttcat caagcttccc tccatatgct
     8281 aattctctta atgtatctac aactgggaat tttcttcctt cttcaattaa atcagctatg
     8341 taaattattt tttctaaaag agacatgtcc tctctccctg ttgtatgata tttgattgca
     8401 tttataatat cttcatcttc tacttcaaat tcatactcgc atatgtacgc cccaattaca
     8461 ctatgagaca aggaacgatt tccatcctct attgggtcta aatatatttc atacttattt
     8521 acataatcct ctatttcctt gtcacttaaa tactttgcac aatcatgaat cattcctgct
     8581 aagtaagctt tttctttgtc acatccataa atctcacata atttttcagc acattttgca
     8641 acattttttg aatggctaag cctccccact ggtagcattt gatttagtct ttggttaata
     8701 ttttctaagt tcatttaatc atctccatat tgatatagtt tattttcata taaataattt
     8761 tctacatttt cgggtactaa gtaacgtatt gacttgcctt catttagctg ttcccttata
     8821 tatgtggacg atatatccag tgatggaaca taaactgtta taatgtcagc attatacttc
     8881 ttagttagtt tttctatagt ctcttgtgac cttaacaaac taattcctgg tctagttgcg
     8941 gctataaaag tagctaactc aaagtttttc tttacatctt tccactcttc aacactacaa
     9001 attgcatctg cacctgttat aaaataaatg tctgcatttt tgtatttttt ctttaagtat
     9061 tttaatgtat ctattgtata agtccttttc tttctttcga tttctacttt tgaaactaaa
     9121 aaatcttcgt tgctttcagt tgctagaagt gtcatattat atctatcata cttatctgta
     9181 gtgatacata atttatgtgg tggatttcct gatggtataa agattatttt gtcaatgtca
     9241 tacttatctc taataaattc tgctgttgct aaatgagcat agtgtatagg gtcaaaagta
     9301 cctcccaata tacctatttt gattttgcct ttatggcgat taaatttctc tagtttttca
     9361 gtatttttca actctgccat cttaactaga ttttcacttc tcatattttg tctcctttta
     9421 atgttataat actattgtac cattattttt tatttcttaa agcttataat ttgttttttt
     9481 gggtatataa ttatatttat atgctaaaaa acacttaaaa aaataattat ttaacatgat
     9541 tctactttac tttttaataa aaataacttt ttactatttt gtcttaattt tgataaaata
     9601 taacatacaa atataagttt tttgttaaat tttattagtt gcttttgtta atataattac
     9661 atataaaaaa ggaggactta tgggtataat tacaaaaata gaacaacaaa aaagaaatga
     9721 tgatagagtt aatatttatg tagatgataa attttttatt gctatattta aagaacttgt
     9781 ttatacattt aatttaaaaa aggggaatga aataaatgaa gaagaattaa aacctatttt
     9841 agatgatgaa atgtatataa aagctaaaaa taaagcatta aatattttat ctcatgctga
     9901 ccaatctgaa aaaaaattaa aagaaaaact atcatcagaa tttgatgaaa atataattga
     9961 gagggtcata gattttctta aaagttataa tctcgtaaat gacagtgtat tagctcaaaa
    10021 aattgttaac acaaatgtta acctaaataa atgtggcaaa aacagaatta agcagaattt
    10081 atataataaa ggtatcaata gaagtacaat caatgaagca gtatctgaat tagacaagga
    10141 cactgaattt gaaaatgcta tgtatctagc aaaaaaaagg tatgaaagag taaagaaaga
    10201 agataaaaag aaaatatatc aaaaaatttc tcaacatctt tcctataaag gctttgatta
    10261 tgatattatt aaaagagttt taaacaaact tttaaatttt gatgaatacg atatataagt
    10321 ataaatacta gggaggtaaa ttatgaaatt aatcattggt ggcttaagct ttttattagg
    10381 attatataat atatatcaat tatacagaga agatagtatg agataaatat tattttttag
    10441 atgtaatatt tattatggag gagaaataca atgaaaagtc aaaaaagatt taaaatttta
    10501 gatattgatg tatacctaca accatttgaa tctgatataa aaaagcgaat gaaacattat
    10561 gaggatatga aatcaaaaac attgaaaaac tataaagatt ttagttcttt tgcaaatgct
    10621 catctttact atggatttca tcagacaaat gatggattct tctataggga atgggctcca
    10681 aatgcagata gtttgtcact aattggtgac tttaataatt ggaatagaaa atctcatcct
    10741 ctaagaaaaa ttgggtctgg taattgggaa atatttattc ctggtcaaaa tagcttacct
    10801 cacaaatcag aggtaaaggt acaagttact gcaaatggaa aaacatttga ccgtatacca
    10861 ttatatataa aaagagtaat tcaaaaagat tttggtggat ttaatggaca aatatggcaa
    10921 ccaaaaactc catttatctg gactgacaat aattttgact taaaaaacat aacttctcca
    10981 ctaatctatg aatgtcatat aggtatgtct actgaaagcg aatctattgg aacttataat
    11041 gaatttactg aaaaaatact tcctaagata aaaaaagctg gatacaatac cattcagctt
    11101 atggctatta tggaacatcc atattatgca tcttttggat accaagtttc aaacttttat
    11161 gcaatctcat ctaggtttgg tactcctgaa gacttaaaaa acctcataaa cactgctcat
    11221 tcaatgggga ttgctgtttt attagattta gttcattctc atgctgtaaa gaatacttta
    11281 gaaggaataa atgagtttga tggaagtgaa catcaatttt tccactcagg ttcaaaggga
    11341 aatcatcctg cttggggtac aaaacttttt aactatggta aacctgaagt aatacatttt
    11401 ttgctttcaa atataaaatt ttggttaaat gagtaccatt ttgatggctt taggtttgat
    11461 ggtgtaactt caatgttata tcataaccat ggtcttggag tttcatttga tagttatgaa
    11521 aaatatttta gtatgaatac agatattgaa gctataacat atctccaatt tgcaaatgaa
    11581 ttaataaaag aaattaaacc taattcgata agtattgcag aagatatgag tggaatgcct
    11641 ggtatgtgta tccctataaa agacggcgga attggatttg actatagact tgcaatggga
    11701 gtacctgatt tttggataaa aactatctct aacttatctg atgaagactg ggatttggga
    11761 aaaatgtggt atgaacttac tactagacga cctggagaaa aaaatatagg atattgtgaa
    11821 tcccatgacc aagcattagt tggcgataaa acaattattt tttggctagc tgacaaggaa
    11881 atgtactgga atatggaaat aaactcaaat aaccatgtta tcaatagagc aatatctctt
    11941 attaaattaa ttaaattaat aactttttct ctagcaggtg aaggatatct aaattttatg
    12001 ggaaatgaat ttggacatcc tgagtggatt gattttccac gagaaggcaa caattggagc
    12061 tataagtatg ccagaagaca atggagcctt tcagaagata acaacttaaa gtataaacaa
    12121 ttattaaatt ttgataaatc tatgctcgaa cttacaaaat acagtgatat ttttatccaa
    12181 aatagtcatg aacttataca tatggataat gataaaaaga ttttggtgta cagtaagggc
    12241 aaatatttat ttatatttaa tttccaccct aatctaatac aatcagttga tatttcaaag
    12301 tttcaaaata caaactacaa aacgatatta aatactgata tggtagagtt tggaggaaac
    12361 acaaaaaaag aaaacttaag agattatgat gtatacttta actcaactcc tccttcttct
    12421 aaaatgccta ttgcctctcg tacagcaata gtgcttttag ataatggtat ttaaatatga
    12481 gccaaatata ttaggaaact gcaaatatag tccagtataa ggcaaaaaaa taaaataatg
    12541 aaagcatggc aatatacctg ttcaacatgc taaaaagaca cattgacaaa tcattatgta
    12601 tttgctaaga taaacatacc aaatgattgt tttgaggagt gagctttttg ggaagaaaaa
    12661 aagaacctga taatactata caaaaaatac ttgatatttc acataagtta ttcttagaaa
    12721 aagggtatga gcagacaacg attcaagaca ttgtagataa tttagatggc ctttcaagag
    12781 gtgctattta tcatcacttt aaatcaaaag acgaaataat taaggcaatc acaagtcggt
    12841 tgtataatga tggattttat gggtatgagg taaaaaatga tgaaggaaag accgcattag
    12901 aaaaattaca atataggttg cttcatgctt atgaacaaca caataaacaa gacatttttt
    12961 taatgtcatc atcactattg cgtaatccta agtatttagc tacgcagttt gatgagatta
    13021 taaatgatat tgtgccttgg ataaaagcat tgattgaaga aggaaatcaa gataattcta
    13081 tggaagttaa aaatccagag attgctgctc aaatgtttgg ttttcttcta aacacatggt
    13141 gtattccaac agtttatcca ggtaatccac aacagatgat agaacgtttt actttgtttg
    13201 gtgagcaagc tgcttttatg ggtatttctt ttatgaatga taagataatg tgtgaatatc
    13261 aaaaatttgt taataaattt tctaaatagt aaatataaaa ctgccttaat atcggcagtt
    13321 ttatatttac acaaaacaaa catttagttg gtatttttaa aaatttaatt tatttcagta
    13381 catgcaaaag gaggttgcta tgaacaaact aattaaagat tgtgtaaact tttttaatga
    13441 ttgtggcttt tcatattcaa tatgtggtgg atatgcccta gaattatttg cgaatatgca
    13501 tattcgccca cacagtgata ttgacatatg gatttctgac acagataaag aaaagagcat
    13561 tcagtatatg cttaatcaag gatgggatat atatgagccc ttgggtaatt atttattacg
    13621 acctattatc gaccttaaca agcaacatat agagcaaatt tgtgtttttg cgattaagcc
    13681 tgattgctca tttattgaat taaaaaaagt cgaggatgat aactataaaa tgaatataca
    13741 gtgtgacgaa cagttacagt tcgattttat tgaattgata cataatccac agaaagatgg
    13801 aaaatttctt ttttcccaaa atcaaagaat tataagagat atggataagg ccagactgat
    13861 aacatcagat aatatcccat atatggctcc cgaagtggtt ttgttttgca aatcaatata
    13921 catagatagg cttggttatc aaaaagactt cgatgtaaca gtcccactat tatcacaaga
    13981 gcaaagggat tggcttgtaa atgctttaat aacagcatat ccagatggac atgcatggat
    14041 tgaccaattg gcaaatcagt attaagactt ttagctcccg aatatgacat tatgataatc
    14101 cacactctaa cagtttgttg gtttttctag tatataacac tcatattaaa ataaacacta
    14161 aaagccaaat aacaatttaa atacaaaatg ttatttggct aatttagttt gatactattt
    14221 aactatcttt tacattcagt tctataattt ccaactttta atttgtggta ttatattcat
    14281 cttaacagtt tgattcttta aagcttttct aaatttctca gttgaagaat ctttaaacat
    14341 tttactagaa gcactcttga ggtcttctat agattcccaa tgttgtatca taatccactt
    14401 ttgagacttt tcatcatata aaagctcagt atcaataaaa ccagattgaa tagaatgaaa
    14461 ttttaattct aagtcattta caatttctat aaactcttct tttgataaat tttcttccac
    14521 attaaactca actatttctg taataatttt catatgtaaa acctccaatt tattttccta
    14581 tatcatatca aaaaataaat tggaggtatt gtaaaaaatc gacagtataa ttataaatag
    14641 ctgtacttta tattttcttt aatcgtctta ttctcaccaa taaatttaat tggtgacgtc
    14701 cctgaaaatt ttttaaattc ttttataaag tgtgcttggt catagtactc attttcataa
    14761 ataaaatcaa aaagttcatc atctttataa ttataaataa gttgattact tgccatttta
    14821 aatctgtaaa ttctctttaa aatcttaggt gtataccctg tatattttaa acagattctt
    14881 tccaatgttt taatatttat atttgtatca tcacaaaatg cctttatatt gctatatcta
    14941 ttaacatata taaatgaatt tagaagcttt acatatttat ttgaaattaa atcttcttct
    15001 aatatggata ataatacttc ttctaaatat aaaacaattt tttctactga ttgtgttgga
    15061 cttaatgact cttccaattt ttttgcaaat agctgtgata ctacttctaa gtcaacaatt
    15121 tgtctgttaa actctgaaat tggtatcttt aaaaatggat ataaaccaaa tggataaaaa
    15181 gaaatcccta taacacgtat atttccatgt tgaataataa atccatgttt atcccttatt
    15241 ccattaaaat gcatattact agcagtatag tcttgatttt caattttata ttccataaca
    15301 tcagaaagat tgagaataat atctatactg tctgtcggaa gtaacttatt atttaacagt
    15361 atatttgatt ttgtctcaaa ataccaaata aactttaccc acggctttaa acgaacattt
    15421 ttgattgaat atcttgtaac tgataacatt gtgaactaac ctctatacta aaatttttta
    15481 attatgtact ccatcctaat attatacagt ataatttatg ctatcataaa ttttagttca
    15541 ctttatattt attttataca gtttttcaat accttagaaa taggaagcgc tattataaga
    15601 cctatcaata cttgcattaa attacctggt attgaagtaa gaggtactat aaaattacca
    15661 taaagtataa cttctgttac ataatatcca aatagcatcc aaattgatga gacacatatt
    15721 cctattacat tgattaaaaa attattccca ttattaccat ttgcccatgc aatctttcca
    15781 agtaaataac ccataactcc tctaactata aaggtaaacg gtgcccataa agtccaccca
    15841 gatatcaaat caaaaagtga cataccaata gcccctgata aagcaccttt tttacctcca
    15901 aatattattg ctgatatgaa caacattgca gttcctaaat gcacaagccc accatttata
    15961 gatattggta atcttatgtt tataaattta gttgcaataa atactaatgc tattaatagt
    16021 gaggtttcca ccatgtcttt cgttgtaaat ttttgcttag ctgtaacttc catactatat
    16081 ccccctatct attaaaaata atacttatta aataaattta acaaatttcc atttcctttg
    16141 aatcctagtg cacttaaact tttatccaaa acatttagta catgttctat tttatttaga
    16201 tttgcattct ctcccatatg acctattctt aaaatagtat ccatatattg atttagtgat
    16261 gttgcaacaa gtgtattata atttttaagc atataatcag taagttctaa agctcctata
    16321 ctttcaggaa cttttatagc tgtgactgta ttagaatatc cttcttctaa aaatagttct
    16381 agaccatatt cctcaataga ttttctaaca ctataagcta tcttttcatg tctatttaat
    16441 acattttcta ttccttcttc taatatatta tcaagtgctt tatcaagccc cattatgtca
    16501 cttattggca ttgtataagg gaagtatttt ttttcataat agccttccca tatatttaaa
    16561 ttacaataaa aacctattac atcagttttt ctatttttta tacaattttt tgcatcttga
    16621 cttattccga ctatagtaag tcctgctggt gcagaaatag ctttttgtga accacctaat
    16681 attatatcta ttttagattc atctacactt aatctttctc ctaccatacc tgctacagaa
    16741 tcaactacag ttaatattcc atatttttta agtaatggac atattttgct tacatcattt
    16801 aatacgccag ttggagtatc acaatgcact actgttgcat atttaaaatt actatcctca
    16861 tctaaaaatg ctttcaattc atcaatatct atacttttag tatattcact tgaaaaaagc
    16921 acatactccc caccatacat tgttacaaaa tctttaaaac cttctccata tataccatta
    16981 tcaattacaa gtactctatc tcctttttct gtaagtgatg cacatgctgc ttcaagacct
    17041 aatattcctt caccactcaa tatgtaaaca tcgttttttg ttccaattat attaccaaac
    17101 ttctcgcatg tctttttata aaattcaaca aaatcaatat ctatatctgg atttgttgtt
    17161 ttttctgctc ttgctaatct tacattttcc ctagtttcag ttggtcctgg tgcataatta
    17221 atcttcttat tcatatatat cagctcctat ttattttcta tacgattatt atacaaaatt
    17281 ttcattttat atggtacaat tttatacttt tttagaattg taccggtaca ttttctaaca
    17341 tgaataatat cagtatatta gatatattat tgattacata ataaaataca gagcaaacat
    17401 ctgtttaaat tgtttgctct gtactatgct ttaacctatt taattatgaa tttctttaac
    17461 tatatttttt tatatgtttt ttgctagtta aactaaccat aaatatttgc attttattca
    17521 ctgtatctta ctctatcaat aagagattct ttattaaaac ttcgtcctat caaataagtt
    17581 gttataaatt gagctataag aatacacaca atcatcaaca agattggtac tattggcaaa
    17641 gaatatgttg cataagataa tccagtcttt tttagaaata taacagcaat ataccctaat
    17701 atacctccaa atagaatact tcctatcatc attccagaag tgtaatacat tgcctctgaa
    17761 ttaagcattt tcactaattg tttattagta agaccaatag cttgaagcat accaagttct
    17821 ttttttctag taactatgct agttatcatt gtgttgacaa gattcataaa tccaatcaca
    17881 cctattatgc ctgttagcgt atatcccatt atctttataa atgaaatagt agccttattt
    17941 gactcaattc tactatctat aaagtcagtc tctaaaaatc cattattttt agcaatcttc
    18001 ttgatactat ctttgatttc ttgatacttt ccttcttctg tatatattcc caagctacta
    18061 gttgaatcgt atttcaaagt tctatctata aaatctttcc ctataccaaa acttggaacc
    18121 cccatagcta ttgcttgaac tttaaattct ttttttatct ttttatctcc atcatataaa
    18181 gttaatctta ttttatctcc tgcttttact cccatttctt ttgctgtatc aacatgagta
    18241 attactattt catctccagt ttgaagtctt tcaagattta tttttccatc tattatattc
    18301 ttgtttaatg attttacatc attttcattt atactttgta aaaggtcata cttaaattca
    18361 acatcatagt cctccatacc aatttttaca cttcttgcag attctattct tttaattcct
    18421 tccatgttct ttatttgatt aaaaaaatct ttacttagag gattatccat ttgtaaaata
    18481 ttcaaattat ctttaggatt tttatcactg ttcatttcat aaccagatag tctaacttct
    18541 atatcataag gaaagtgttc tcttgccatt ttctcagcat caaaactttc taaggcagtt
    18601 gatactgtta taaatatagt tccactaagt ataagtgatg ccaatgtcat gtaagtacgt
    18661 tttttatttc tttctaaatt ggcatgtgat aatctcttta gatttatatt tttatatcct
    18721 tttctcttac tcttattact tattttattt cctgtatatc ttactgcgtc aacaatggat
    18781 acttttgatg ctactttcat tggttttaaa agtgataaaa caacagatat aaagcttatt
    18841 accacaaccg ataatataac tggtaatcgt gatgatttta catctatatc cataaatgat
    18901 tttattataa tatttgcaag tacatagcct aaaattattc ctattggtat tgctattccg
    18961 gctaatatga acccttcttt aaacactata ttttttattt gtttcttagt tgcaccaatt
    19021 gctctaagtt ttccaaattc ttgaaccttt gtaactattg aaatataaaa tatattgtat
    19081 attactaaaa tactagataa tacaattact aaccctacaa aaactccacc cataatcgtt
    19141 tccatatctg gttttaaagc atttatataa ttttcattta cagcagtatt aatagtatcc
    19201 agtcctatat cattagcaat tttatctaat ttttcttttg cttgctctat tgataaatta
    19261 cttttatcat taaatttcac ataaatatta aaatcttctt gtgacatatc tctagtattt
    19321 ctcatatatg attctgatat aatagcataa taatatttac ctgcttcaga tatttcgcta
    19381 gtttttaaga ttccacttat aatgaactct ttttcaattt ttttatcatt tttatagtca
    19441 tcataagata tttttatctt ttcacctaaa tctttatttt tataaccaag tgcctttaaa
    19501 gctccactat ctaacacaat ttcattctct tttgatggca attttccttt gataaattca
    19561 aaactattta atttcaaagc atcttcattc atgtatgcta gagaaatatt tgaatcatct
    19621 tccagttttt caacacctac tccattcatt tcaccagtta attcaacatc tgcatgatta
    19681 ctcaatatat cagcctgttt ttcatctaca tttgctaata taccttgata attacctgaa
    19741 tactttatag tgttttcttt ttgatatgct cctgcatcaa caatataaat tccaatagaa
    19801 gaaagcatta aggttgaaag tattatagta attattatta gaatactttt tgatttattt
    19861 tgctttaaat tactttttgc tattttagta gtattaatca tttttatcac caacctttga
    19921 caccctgcca tcttctatga atataactct atcagccatt tgggctatac tgtcatcatg
    19981 agttatcata actagagttt gactgtactt cttagacata gattttaata tactcataac
    20041 ctcatcagac gttttagaat ctaggtttcc agttggttca tcagcaagta ttatagctgg
    20101 tcttgtagct aatgctcttg ctattgccac cctttgttgt tgaccacctg ataatgtatt
    20161 tggtaaaacg tcgtgtttat tttcaagtcc taatgatttt aaaagttctt ttataaaaga
    20221 ttcatctact tctctaccat ctaaaccgat tggcaacact acattttccc atacattaag
    20281 agatggaatt aaattatagc tttgaaatat aaaccctatt tttcttcttc taaatacagc
    20341 aagttcttct tcttttaatg taaatatatt tttattatct atatacacct tcccagatgt
    20401 aggtatatca agacctccaa tcatatgcaa aagtgtactt tttccagaac ctgattttcc
    20461 tattattgca acaaattcac cttcatttat ttctaagtca acattgtcca aagctcttac
    20521 taagctttct ccttttccat aatgctttga taaattttca gtatatagta ttttcataaa
    20581 attatcctct cattctaagt tatattactc catattttat taatttttta tttctggagc
    20641 tgtttatact tatataataa atgtagaggc ttacattttc ctttcattta atattacata
    20701 gttgtaagaa acaaagaaaa cttgctccct attcctactt ttgaagaaac tataatattt
    20761 ccaccttgtt cttctaaaat ctttctactt aaatacaatc ctaccccact accttctaca
    20821 gattcaattt catcagcaga acctctataa aaacgtttaa atatattatt aaactcattt
    20881 ttttcaatcc ctatgccatt atcctctatt ataattttaa agaaattagc agtttcttct
    20941 acagatatat ttatttctcc atttacctgt gtatacttta cagcattgtc taatacatta
    21001 aaaatactct cctcagtcca ctttttatca tgtggtataa ttctactgtt aaattcattt
    21061 aaatttaatg ttatactttt gctcttagcc ttaggggtaa cactttttac tgcatttaga
    21121 atagtatttt ttatatcatt atcttctttt tttatgttta tcatgctaat ttctaacctt
    21181 gatatgttca ctaaagaatt tattaaattt tctaatttat ttatagaagc cttattggtt
    21241 aataagaact cgttggcttc atcttcttct aattcttctt ctataagtaa tgtattgtat
    21301 aattttaagg atgctaaagg agtttttaat tgatgggaaa tatctgttac taatgatttt
    21361 acactttctt tttctttttc taatttaata aactttatct ttaaacttct attaagctca
    21421 tatagacagt tatttatctt actaaatatg ccttctttaa agtattcatt atattctact
    21481 agataaccag tttcttctga ctcagtagtg tccttcaagt agttttcttg tgacatctta
    21541 tctactataa aataaatttt actcaatctt ttatttatgt atcttataaa ataatatatt
    21601 atacctataa ctactataaa catcattaaa aaaataaaaa atgattcctt aaaaaaagat
    21661 tttaagtata agctaaaatt tttatcattt gacattttat tttccaatcc atagccaaat
    21721 ttcttaaatg ccttttctcc agtagacacc ttactataat cagaattata aatagaattt
    21781 actatatcta tttcatcatt aggatatttt tcaatcaata ttccagcaat attttgagta
    21841 ttttttattt gatgttcata caatttatgt aatctgctat attcaaaaag tataaataca
    21901 agggttacta ttagtacaga taaaacagaa tatacaataa ctcttctcat tatataatca
    21961 tctttcacac ttaaaccatc tttgaggttc atatttttta gcattttttt atactccttt
    22021 ctccccacat atatccaacc cctcttactg tttttatata tttaggttca gatggatttt
    22081 tttcaacctt ttgtctaagt cttcttatat ttactgcaat cgtattatca tctacaaaat
    22141 taccactact atcccataaa gattctaaca gttgctcttt agtcaatgtc tgcatagaat
    22201 tttccatcaa atattttaat agttttgtct ctgtcttact taaaattatc tcttcttcct
    22261 tatcatctgt tcttataatt aatttatttt ctatataata aaagaataaa tcatcacaaa
    22321 ctaatagagt ataatttttt actgtattta ctcttttcat aagcgcattc acttttgata
    22381 ttaataccat caagctaaaa ggctttgtga tataatcatc tgcacctaaa tcatatcctg
    22441 tgacaatatc cacctcttca tcaagagctg tcaacattat tatgtaaaca tcacttttct
    22501 ttctaatctc ctcacaaaag tcaaaaccac taccatcagg tagacctata tcggaaataa
    22561 ttaagcatat ctcttctttt gcgaaaatat tttttgcttc ttctatacca aatgcactaa
    22621 aaacattgta tccttctttt tttaatttaa aactaattcc tctatttaaa gatttgtcat
    22681 cttccaaaag taatatatta ttcatcccta tatcctccat catcatatat actagctaaa
    22741 ttatatcata ctaataaaat cattttttta tcaatttgta aatatatcag aagttgttaa
    22801 tatgatttca tttacttgaa taaaaactaa aaatccccat aatactatgc catttagtgt
    22861 aattattttt cataaaatac aattttattt aaaagtaatc atgttaaaac ataaaacaaa
    22921 ggaaattagt atttatataa ctaatttcct ttgctgttta ctattatcta aaattaaatt
    22981 actatctact aatttttgct cattaatttt tat
//
LOCUS       DAKBNN010000044        21942 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000044 DAKBNN010000000
VERSION     DAKBNN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.044"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="OUB02_001470"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="OUB02_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCT8885233.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="OUB02_001471"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="OUB02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCT8885234.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="OUB02_001472"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="OUB02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCT8885235.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="OUB02_001473"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="OUB02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCT8885236.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="OUB02_001474"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="OUB02_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCT8885237.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="OUB02_001475"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="OUB02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCT8885238.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="OUB02_001476"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="OUB02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCT8885239.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="OUB02_001477"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="OUB02_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCT8885240.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="OUB02_001478"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="OUB02_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCT8885241.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="OUB02_001479"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="OUB02_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCT8885242.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="OUB02_001480"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="OUB02_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCT8885243.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="OUB02_001481"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="OUB02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCT8885244.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="OUB02_001482"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="OUB02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCT8885245.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="OUB02_001483"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="OUB02_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCT8885246.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="OUB02_001484"
     CDS             6989..7174
                     /locus_tag="OUB02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCT8885247.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="OUB02_001485"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="OUB02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCT8885248.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="OUB02_001486"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="OUB02_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCT8885249.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="OUB02_001487"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="OUB02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCT8885250.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="OUB02_001488"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="OUB02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCT8885251.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="OUB02_001489"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="OUB02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCT8885252.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="OUB02_001490"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="OUB02_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCT8885253.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="OUB02_001491"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="OUB02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCT8885254.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="OUB02_001492"
     CDS             11117..11767
                     /locus_tag="OUB02_001492"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCT8885255.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="OUB02_001493"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="OUB02_001493"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCT8885256.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="OUB02_001494"
     CDS             12536..12805
                     /locus_tag="OUB02_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HCT8885257.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="OUB02_001495"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="OUB02_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCT8885258.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="OUB02_001496"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="OUB02_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCT8885259.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="OUB02_001497"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="OUB02_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCT8885260.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="OUB02_001498"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="OUB02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCT8885261.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="OUB02_001499"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="OUB02_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCT8885262.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="OUB02_001500"
     CDS             14901..15848
                     /locus_tag="OUB02_001500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCT8885263.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="OUB02_001501"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="OUB02_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCT8885264.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="OUB02_001502"
     CDS             16330..17163
                     /locus_tag="OUB02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCT8885265.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="OUB02_001503"
     CDS             17151..18017
                     /locus_tag="OUB02_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCT8885266.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="OUB02_001504"
     CDS             18011..18814
                     /locus_tag="OUB02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCT8885267.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="OUB02_001505"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="OUB02_001505"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCT8885268.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="OUB02_001506"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="OUB02_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCT8885269.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="OUB02_001507"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="OUB02_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCT8885270.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="OUB02_001508"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="OUB02_001508"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HCT8885271.1"
                     /translation="MEYYGVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKALSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8811 a         2563 c         4498 g         6070 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataga atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaagggg ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagttt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaactaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgaa
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtaga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcttt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aagtttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa taggggcaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattatg gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagcat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAKBNN010000045        21684 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000045 DAKBNN010000000
VERSION     DAKBNN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.045"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            165..1595
                     /locus_tag="OUB02_001509"
     CDS             165..1595
                     /locus_tag="OUB02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HCT8885272.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            1963..2541
                     /locus_tag="OUB02_001510"
     CDS             1963..2541
                     /locus_tag="OUB02_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCT8885273.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2571..2717
                     /locus_tag="OUB02_001511"
     CDS             2571..2717
                     /locus_tag="OUB02_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCT8885274.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3022..4401
                     /gene="scfB"
                     /locus_tag="OUB02_001512"
     CDS             3022..4401
                     /gene="scfB"
                     /locus_tag="OUB02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HCT8885275.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4430..4927
                     /locus_tag="OUB02_001513"
     CDS             4430..4927
                     /locus_tag="OUB02_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCT8885276.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5225..6889
                     /locus_tag="OUB02_001514"
     CDS             5225..6889
                     /locus_tag="OUB02_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCT8885277.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6902..9352
                     /gene="recJ"
                     /locus_tag="OUB02_001515"
     CDS             6902..9352
                     /gene="recJ"
                     /locus_tag="OUB02_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCT8885278.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9365..9877
                     /locus_tag="OUB02_001516"
     CDS             9365..9877
                     /locus_tag="OUB02_001516"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCT8885279.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            9951..12158
                     /locus_tag="OUB02_001517"
     CDS             9951..12158
                     /locus_tag="OUB02_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCT8885280.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12183..12632
                     /locus_tag="OUB02_001518"
     CDS             12183..12632
                     /locus_tag="OUB02_001518"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCT8885281.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12647..13261
                     /locus_tag="OUB02_001519"
     CDS             12647..13261
                     /locus_tag="OUB02_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8885282.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13352..14908
                     /locus_tag="OUB02_001520"
     CDS             13352..14908
                     /locus_tag="OUB02_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HCT8885283.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIKNMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14898..16160
                     /locus_tag="OUB02_001521"
     CDS             14898..16160
                     /locus_tag="OUB02_001521"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCT8885284.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16234..18021
                     /gene="aspS"
                     /locus_tag="OUB02_001522"
     CDS             16234..18021
                     /gene="aspS"
                     /locus_tag="OUB02_001522"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCT8885285.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            18480..19712
                     /locus_tag="OUB02_001523"
     CDS             18480..19712
                     /locus_tag="OUB02_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCT8885286.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            19726..20532
                     /locus_tag="OUB02_001524"
     CDS             19726..20532
                     /locus_tag="OUB02_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HCT8885287.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(20644..21513)
                     /locus_tag="OUB02_001525"
     CDS             complement(20644..21513)
                     /locus_tag="OUB02_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8885288.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         8679 a         2214 c         3968 g         6823 t
ORIGIN      
        1 tattaacttt attaccccac atatataaga taaaattatt aatataaaat ataatctatc
       61 gacaatgaat agacttttaa ataaacttaa tataaaatta attaaataag tgcttaaaaa
      121 cgatttactt taatgataga atcatattaa aggaggataa ttttatggat ataaagaata
      181 aaattaaatc tatattttta ccagaaaaaa atgactattt agatgatgaa gaatttgagt
      241 atgatataga aaataatgaa gaagatttaa aggaagataa caaagtacat ttaaatgatt
      301 taagcagagt aaaatatgat tacagagagt taaaagacat agaaaaccaa acagaagaga
      361 tgtgtctgct agcagtaaag caggatggta ctataattga gtttgtaaag gacaagactt
      421 ataaagtatg tatggaagca gtaaaacaga catataaatc tcttaagtat ataacaaatc
      481 aaaatgagga tatttgtata gaagcagtaa aacagaacta cagagctttg tactacataa
      541 ataataagac agaaaatgta ctaattgagg ctataaaaaa tgcatcaact tatgatgtca
      601 tggaagtatt taaatttgtg gaagagcaaa cagaagatgt atgtctagca tttatagaaa
      661 gggcatctaa aaatgatgtt gcagagatat taaaaggaat aaaagagcaa actccagcca
      721 tatgtttaga agctgtaaaa aaagatggaa aatctttagc ttatgtaaag gaacaaagta
      781 atagtatatg tttagaagct gtaaaagaaa actacagtgc actatcttgt gtaaaagagc
      841 aaactgaaga aatatgcata gaagcagtaa aacagaatga ctttgcattg tattatgtaa
      901 atgaacagac agaaaaaata tgtatggaag cagtaaaaag aagttatatg gctctacagt
      961 atgtaaataa gcaaacagaa gagatttgtt tggaagcagt aagaattgat ggaagagcat
     1021 tacagtacgt aaaagagcaa acagaagaaa tttgcttgga gtcagttaga caaaatggaa
     1081 aagtactgca atatgtaaaa aaacagactg aaaatatatg tatagaagca gtaagaggtt
     1141 catttgaaga acttgaaata aaagaaatat taagttatgt aaagatacca acagaaagaa
     1201 tatttgtaga agcagtaaag caaaatggaa aaattcttaa atatgtagaa aatcagacag
     1261 aattaatatg tttagaggca gtaagagaaa actataatgc tttagcttat gtaaaggaac
     1321 aaacagaaaa gatttgttta gaagcagtta atcaaagtta tgaagcgctt aaatatgtga
     1381 aagaacaaac agaagaagtt tgtctaaaag cagtaaaaca agattataga atgttgaaat
     1441 atgtaaataa tcaaacagaa aaaatatgtt tagaagcagt aaagcaaaat tatagagctt
     1501 tagaatttgt tgataatcaa acagaaaaag tttgtcttga agcagtaaaa cagaatagaa
     1561 aagcactaca gtatgtaaaa caaaaacaaa gttaaaaggc taaaatatat agaaaatata
     1621 tgcaaataaa taaaatttga tattttgtaa taaataaaat gggtatctca aaatgaactt
     1681 tttagtttgt gagatactct ttttatatga aataaaatat atttttagcc ttttaacaat
     1741 agggaaaagg tttatttcta ttttgacata ggctttttat gcatataaag cttgataaaa
     1801 ttgttttatt ataattgttt tgttatatga ataataagaa gaaaattata taggtctttt
     1861 tttgaccagt tttacatttt taacaactag ttttgtaaag tatattgaat tttctcaaaa
     1921 aagaatgtac tctatatatg taaaattttt ttggaggtaa aaatgtttaa aagatatgct
     1981 gaaaaaatga catctgtttt aatttgtaat aatatgatag ataataatga atctaaagtc
     2041 tattcttatg gatttgaaat attaatagct ttcatagtta atataaccac catgctgttt
     2101 ataggttttt tatttggtaa atttacatat gtattatttt ttttaatgtg ttattgtcca
     2161 ataagacaat tttcaggagg gtatcatgca gacaattatt ttagatgctt attaaccttt
     2221 atttttataa ttttatctac aatcttaatt atagaaaata taaatataga cttatttaaa
     2281 aatattatta tgattattgc aagtgttagt tgggttggaa tttgtgttct gtgcccaata
     2341 gaacatagga gtaaccctat aagtgatagg gaaaagttgg tttataagaa aactgctata
     2401 tttatatcta cagtagtgtt attgataaca attttaagtc tttctttgag tatatttgta
     2461 gattatttca catatagcgc atttgccatg ttttggattt ttgtgatgtt agtattaggt
     2521 aagttaaaag ctaaagtata agtaaaatat gtatgaggga gagttaagtt atgaaaaagt
     2581 ttattgtaag atttatgaag tttgctagct cattggcact tagcactgct attttgtcag
     2641 ctaatagtac ctgtccttgg attattcatc agcctaaagt acccaaagaa atcagcaatc
     2701 taaagaagac aaattaaata caagcactta tttatacaaa attatcagga aaaaagaaat
     2761 tattatattt taataagatt aaaacaatat aaaatcagat acaaaattta ttttatttat
     2821 tttataggag cctaatacct atttggtaat atattagtgc gccaatttag ggggatatag
     2881 tgtatttaat gaaatgactt acggagagta agtcattttt gtattgtatg tattttatgt
     2941 aaacttatga tataatgaag ggtaaagttt aattaaaata ataaaaaact ttagtataga
     3001 tagtaattta ggaggtaggg tatgagtatg atacataaat tttctatgaa tggatataat
     3061 atcgttttag atgtgaatgg tggagctgtt catgtgttag atgatgttgc atacgatttg
     3121 ttagattttt ataaagaaaa aagtaaagaa gaaatattag agatattaaa atcaaaatat
     3181 caagaagaaa aaataaatga agcatatgaa gaaatattaa atttggaaaa agagggtctt
     3241 ttatatacag aagatacata tcaatatcat ccaagctttg tacatagaga gcctgtagta
     3301 aaagctttat gtttaaatgt agctcatgat tgtaacttaa aatgtaaata ctgttttgca
     3361 gctcaaggtg attttggagg agaaaaagaa cttatgagtt ttgaagttgg taaggcagca
     3421 attgactatt tgattgctaa ttcaggaagt agaaagaacc tagagataga tttctttggt
     3481 ggagagcctt tgatgaactt tgaagttgta aaacaactgg ttgactatgg aagaagtgta
     3541 gaaaaagatt ataataaaaa tataagattt acaataacta caaatggtgt actgctaaat
     3601 gatgagatta tagattatat aaatgaaaat atgcataatg tagttttaag tttagatgga
     3661 agaaaagaag ttaatgataa tatgagacca actttaaatg ataaaggaag ttatgatatt
     3721 acattaccac gatttaagaa acttgtggaa aaaagagcaa aagataagta ttattatata
     3781 agaggtactt ttacaagaga taatttagat ttttcaaaag atgttatgca ttttgcagat
     3841 ttaggattta aattaacctc tgtagagcca gtcgttggag atgagagtaa tccatatgca
     3901 cttagagaag aagatttacc taagatattt gaagagtacg aaaaatttgc tgtagagtat
     3961 gcagatagac aattacaagg agatggattt aaatttttcc attttatgat agacctaaat
     4021 caaggtccat gtgtaataaa gagaataact ggatgtggag ctggaaatga gtatctttct
     4081 gtaactccaa atggagatat ctatccatgt catcaatttg ttggaaatga agaatttaaa
     4141 atggcaaata tatttgatga agaaatagta cttccagaaa atttaaaaaa tatgtttaga
     4201 gaagctcatg tatatactaa agaagagtgt aaacaatgtt ggaataagtt ttactgttca
     4261 ggtggatgtc atgcaaatgc gataaacttt aataatgaca tatcaaaacc atatgaatta
     4321 ggatgtgaaa tgcaaagaaa acgtacagag tgctctataa tgatacaagc taagttaatg
     4381 ttagaaggag caaccaacta aatttatatt ttaaaggagt tgtttatata tgattagaga
     4441 ttatttagaa gataaacctt taattgacga aagtgtattt gtggcaaaat cagcagatgt
     4501 aattggaaat gttaaaatag gcaaagattc aagtatatgg tacaatgcag tagttagggg
     4561 tgatgaagga ccaataacta ttggagaaaa cactaatata caagattgtt ctatagtgca
     4621 tggagataca gaaactataa ttggtaacaa tgtaacagtt gggcataggt ctatagttca
     4681 tggttgtaaa attagtgata atgtgttaat tggtatgggt tcaataatat tggataatgc
     4741 tgaaatagga gaatatactc tgataggagc aggtacacta attacatcta ataagaagtt
     4801 tccaccaggt gttttgataa tgggttctcc tggtaaggtt gttagagagc ttacagaaga
     4861 agataaaaaa tatattgatg aatcttatga atggtattta gaggcagcac aaaatcaaaa
     4921 atattagttt aaagagtggt tgtttcaaaa catatttaaa tctgttttga ggcaaccact
     4981 aatttatttt attagatttt gttgatattt tagttttcaa aaagtatttg attaaaccag
     5041 atataaaatg tcaaaaataa aaattaactg agttaaataa ttttaataaa attggtagta
     5101 tatgtgataa aattaaatat taaagaggat ataggatatt agaatatcaa ttggtgaaat
     5161 aatgtcatat acgataaatt aaggtgtaag tttttgggta tacctatctt aggagtgaaa
     5221 agatttgaga ataagttata gaaatttaaa gcgatataga gaaattggat atgttttaat
     5281 aaagtatggt tttagtttta ttgtagaaag actaaatata gaaggaattg cctataaaat
     5341 accactattt gacccaccag aagaaataaa aaatatgaca actggtgaga gaatgaaaag
     5401 agttttagaa gaactaggac ctacatatat aaaaatagga cagatattga gtacaagaaa
     5461 agacttgtta gaccaggata taattgatga aatatcaaaa ctcagagatg atgtagagaa
     5521 atttgattca aatatagcta ttgatatatt taaagaagaa gtgggtttaa gtatagaaga
     5581 aatattctta gaatttaaag aagaaccaat agcagcagct tcaattggtc aagtttatga
     5641 aggtgtactt aaaacgggag aagaagtaat cgtaaaaatt caaagaccaa atatagagaa
     5701 aataataaaa tctgatttag agattcttag aactattgca aatactttaa aagatttaaa
     5761 aaaagatttt aatttagatt tagtacagat gattgaagaa tttcaaactc aattaatgag
     5821 ggaacttgat tatacatttg aagctataaa tgcaactaaa ttctctagga tatttaaaaa
     5881 tagtgatgaa gtatatatac ctaaagtata ttcagaatat aatactaaga aaattttagt
     5941 aatggagaaa gttaatggta caaagttaag tgatgtagaa aaaataagga gactaggtta
     6001 taacacaaaa actattgttg aaataggcgt taggtcattt tttacacagg tattatctca
     6061 tggatttttt catgcagacc cacatccagg taatatattc gtagttgcta aaaataagat
     6121 agcatatata gattttggaa tgattggaat tattgataat aaaactttaa accaattaaa
     6181 tgaaattgca ttagcaggtg tagaaaagaa tgttgacaag ataatatatc tattgataga
     6241 aatggatgca ttaaatggag aggctgatat taagggactt agacaggatt tactttatct
     6301 aatacattat tactatgata tttctataga aaaaataaat gtaacagata tattaaatga
     6361 actatttagg ttttttagac agtataaaat agtcatgcct gcacaatttg taactcttgc
     6421 aaagacagtt ataacattag aaggaacaag tagaacttta aacacagatt tttcttttgg
     6481 ttctatggga aaagagttta tgaaacatca ttataagagt aaatttaacc ctaaaaatgt
     6541 tgttttaagt tcaagacaaa atgtagaaga aatattactg gacataaaaa ctattccaaa
     6601 gcaattaaaa gcaattttaa aaaatataga aagaaataat ataaaaatgc aaattgaaga
     6661 tgttaaaatg acgaggttag aaaattgtat aatagagcta acatctcaaa tatctttgag
     6721 tttagtgttg gcatctatta tagtaggttc ttctcttata attgcatctc caaatataga
     6781 aaataatata tggataaaat ttactgcaat agcgggtttc tttatatcct ttataatagg
     6841 cctttgctta gttataagaa gtattaggtc aaaatacaaa aaagattagg gggaattaaa
     6901 tttgaaaaaa tggatattga agtataaagg taagatagag gaaagtgaat ttagcaaaaa
     6961 acttaatatt tccccggaaa tctgccaaat tttaaaaaat agagggatat gtactgaaaa
     7021 agattcagaa atatttatga atccatcttt agattattta agagaccctt ttttaatgaa
     7081 agatatgcaa aaagctgttg atagaataaa gttagcagtt gagaagaacg aaaaaatttg
     7141 gatttacgga gattatgatg ttgatggtgt atcatctaca tctatcttgt gcttatattt
     7201 taagagtata ggttacaatg ttgattacta tattcctaat agattagaag aagggtatgg
     7261 tataaatgaa gatgctatta agcttataag ctctagggga tgtgacttga ttataagtgt
     7321 tgactgtgga attacatcag tgtctgaggt caatacagcg aatgatttgg gcatagatgt
     7381 tataattaca gaccatcatg aatgtcaaag tgaaattcca tcagcctttg cagtaataaa
     7441 tccaaaacaa gaagattgta attatccatt tgattcacta tgtggatgtg gtgtagcatt
     7501 taaaatgatt caagccttga ctccaaaaga agaatttaaa actagtatgt ataattattt
     7561 ggaaatagta acactagcaa ctatttgtga tattgttcca cttatagatg aaaatagaat
     7621 aattgtaaaa aatggtttaa aatcaatgaa agaaggcaaa aatattggat taagggagct
     7681 tataaaagtt tgtggtgtag aaagtgataa gataggttct tctcatatag gttttgcaat
     7741 aggtcctagg ataaatgctt caggtcgatt gggatattct tacttaggag tagagctatt
     7801 tactactcaa tcacaagaag aggcagtaga aatagctagt atattggaag aaaaaaataa
     7861 tgaaagacag atgatagaag ctaagatgta tcatgaagct gaagaaatgc taaagtctaa
     7921 tagcagatac aatgatgaca aggtgttggt tttagctaaa gagggttggc aacatggaat
     7981 aataggaatt gtagcatcaa agttaactga aaaatactac aaaccaacaa ttcttttagg
     8041 tatagaaaat ggggaggcta ctggttcagc gaggtcaata aaaggattta atatatttga
     8101 agctctcata aagtgcaaag acttgatgac taagtttggt ggtcatgaac aagcagcagg
     8161 gttatccttg gattcagaca atgtagaaat tttagcaaat gaaataaata aatttgcaga
     8221 ttataattta actgaagatg atatgataga gaatgtaaat gtcgagtttg aattacaaga
     8281 aaatgtaata aatttaaatc ttgtagaaga attacataag ttagaaccct ttggattaaa
     8341 taatcctaat cctagattta tagttagaaa ttatatttta aaagatttga aagttatagg
     8401 taagaatcaa caacatctga aattaagcat agaaaaagag aaaagctatg aatgtatagg
     8461 atttaatatg agtcatttaa aatcaatgta taaagtagga gacaaggtag atgtactatt
     8521 ccaattagat gaaaataact atatgggaaa taggaaagta caatttttac ttaaagatat
     8581 acgattggct cgacctaaaa gtgcaagtaa tgacaaactt tcattgaaat taatgtcaaa
     8641 aataatacca aaagatactc aaagcctgta taatatatct gtgtctgatt ttgaattatt
     8701 tgatggaaat acagatataa atatatttga ttattttgaa aaagatacac taataatatc
     8761 taattctata aatggatttt atagagcaat gtcagatata tcacttatag atttggattt
     8821 taacataaat tacaatatta ttgaagatgt tagcaaaaat actgataaat tagagcttat
     8881 attttctcct aatattgata aaatagactt aaaaagatat aataatatta ttctttatga
     8941 ttatttatac aataaaggcg aatattcgta tatatatgag aataaaagag aagaaagtga
     9001 aattataaaa tattataata aaacagacct attatattta aagaatgtag ttagtaatat
     9061 agttccatca agagatgaat ttataacaat ttataaacaa gcactcataa aaaaagaaat
     9121 tgatttggat atggtaaata taagggaaac ttttaatgta attccattaa agttttttac
     9181 gatattaaat gtatttagag aattaaattt acttgacttt aatttaaatt atgaaaaaaa
     9241 ttctgtactc ataagaatat tgccaaaacc acagaaaaaa ttagacttaa atgagagttt
     9301 aatactcaat aatctaaaaa atttagaaaa acaatacaat agcagttatt aggaggcgtt
     9361 tcccatggat ttaaaaaact ttataaggaa tattgatgat ttcccaaagc caggcataga
     9421 ttttaaagat gtgacaactc tatttaaaga tggagatgca tttaaatatg cagtggattc
     9481 tatagttgaa gaattgaaag ataaagatgt agatttagta ataggaccag aagcaagagg
     9541 atttttgatg ggtacacctg tagcatatgc tttaggagta ggatttgttc caattagaaa
     9601 acctggaaag ttaccaggtg aagttgagag ttatgagtat ggattagagt atggtacaga
     9661 tactttagag atacataagg atgcaattaa aaagggtcaa aaagtagcaa tagtagatga
     9721 tttattggct actggtggaa ctatggaagc cgctgccaag cttgtagaaa aattaggtgg
     9781 agaagtagtt tctatgcaat ttttgattga gctaaaattc ttaaatggga gagaaaagtt
     9841 gtctaactat gatgttaatt ctcttataaa atactagtac ttttataata gttggtataa
     9901 caccaactat tttcatatat attaaaattt tatacaaaat aggtggttat atgcatgata
     9961 aagaattaca agaaataatt gaaaaaataa aagtttatgc acctaatgca aattttgaaa
    10021 tggttgaaaa agcctataat cttgcaaaaa aagcgcatga aggacaatat agaaaatctg
    10081 gggaaccata ctttattcat ccattagcag ttgcaaatat tttaatagag atggagttag
    10141 atattgaaac tataacagca ggtttacttc atgatgttgt agaagataca gaatatacct
    10201 atgaagacat aaaaaaagaa tttggcaaag aagtagcgga tttggttgat ggagtaacta
    10261 aacttggtca aataaaatat cgttcgaaag aagaaactca atctgaaaat ttaagaaaaa
    10321 tgtttttggc aatggcgaag gatataaggg ttatacttat aaaacttgct gatagattgc
    10381 acaacatgag aacattaaaa tacatgcctc cagaaaaagc aaagtataaa gcaaaagaga
    10441 cacttgaaat ttatggtggt atagctcata gattaggaat ttccaagata aagtgggaac
    10501 tagaagacag agcattaaga tttatggacc cagaagggta ttatgattta gttagtagag
    10561 tatcaatgaa gagaagtcaa agagaggatt atattcaagg aattgttgaa cttcttaaag
    10621 ataagtttaa agatttaagt ataccatgtg atgtatatgg tagaccaaag catttttaca
    10681 gtatatatag aaagatgcaa aaaaaacata agacttttga agagattttt gatttaacag
    10741 cagtaaggat attggttgat aatgtaaagg attgctatgc agtacttggt atagttcata
    10801 cattatggag acctattcca ggaagattta aagattatat agcaatgcca aaacccaata
    10861 tgtatcaatc attacacacc actgttgtag gtcctgatgg agaaccttta gagatacaaa
    10921 taagaactca tgagatgcat aatatagctg aaaatggtat agcagcccat tggaaatata
    10981 aagaaggtat ctctagcaaa gagtcaaagg ttgaagaaaa acttcaatgg ttgagacaga
    11041 tgatggagtg ggaaaaagat ttaaaagacc cacaagagtt tatggatgca cttaaagaag
    11101 atgtatttaa tagtcaagtt tatgtattta cgccaaaagg agacgtaatt gaattaccag
    11161 cagaatcaac acctatagat tttgcctata gggttcatac aaatgtaggt aataaatgtg
    11221 taggagctaa aattgatgga agaatagttc ctattgatta taagcttcaa aatggtaata
    11281 ttgtcgaggt acttacatca tctaattcta atggaccaag tagggattgg cttaatatag
    11341 tgaagacccc aaatgcaaaa agtagaatta gacaattttt caagaaggaa agaagagaag
    11401 aaaatgttga ccgtggaaat acaatattag aaagagaatt taagaagcat gatataccac
    11461 aaaaagaccc tcttgttgaa aaatatatgg cattagttgc taagaaattt aatcagccaa
    11521 gtgttgagga cttaattgcc actgtaggtt atggtggtat aatgtcatct caagtggtat
    11581 ctaaggtaaa agacctatat ttaaaagagg ttaaaaaagc taaaaaggaa caacagatac
    11641 aagaagaaga tttaaataaa cacaatataa gtgaaacaga atacagtaaa aagagaaaga
    11701 aaaacgcatc acaaggaatt atagtaaaag ggcttgacaa tatcttagta agatttgcta
    11761 agtgttgtaa tcctcttcca ggagatgaaa ttgtaggata tattactaag ggtaggggag
    11821 tagccataca cagagctgat tgcccaaatg caaacaataa tggtgatttc ttccaaaata
    11881 gattggtgga ggtttcatgg agttcagcag gaaaaggtaa gtttgaagcg gaaattcaaa
    11941 taaaagctat tgatagaaga ggaattgtta atgaaataac tcatattgtt gcaatggata
    12001 aacttagttt aaacggaata aatgcaagaa aaggtaagga tagtattgta agtataaact
    12061 tacttgtaga ggttaatgac acagaggaat taaaaattct aatgaaaaaa ttaaaagcaa
    12121 ttcctggtgt agaaagtatt tatagaatga caaattagag ttaaaataat agggaggaaa
    12181 ctatgagagc agtagtgcaa agagtatctt catctaaagt tacagtagat gaaaacacta
    12241 taggtcaaat aaataagggg ttactagtat tgttaggagt gactcatgat gataaatcaa
    12301 gtgatgtaga ttatatgatt gataaaatat tgaatctgag aatttttgaa gatgaaaatg
    12361 acaagatgaa tttatctcta atggatatag gtggagagct tttagtagta tctcaattta
    12421 ctttatatgg agattgcaga aaaggaagaa gacctggatt tagtaatgca gctagaccag
    12481 aacttgcaaa taacctttat gaagaatttg taaaaaaagc aaaagataaa ggtgtgacag
    12541 ttggtacagg tcaatttgca gctcatatga tggtagagtt aactaatgat ggtccagtaa
    12601 caatactatt agactcaagt aaatcttttt aagggggaaa actttaatga ttataaaaaa
    12661 atttgtagat agttattttg gagtaaatac ttatgtatta ggtgatgaaa agactaaaaa
    12721 atgtgcagtt atagacccag gtggaagttt agtagagaca ttaagttttg taaaagaaaa
    12781 tgaattaaat attgaatata ttatccttac tcatgggcat ggagaccata taggatatgt
    12841 taaggatatt aaagaaaaaa ctggtgctaa agtagttgca catgttgatg aaaaagaatt
    12901 attaaatgat aaaaataaaa atctaagtta tacaatgaga tgtggagcgc aagagtttga
    12961 tgcagatatc tatgtgaatg ataaagacaa gcttgaactt ggtgaattaa aaatgacttt
    13021 cttgcatact ccaggacata ctcaaggatg tatgtgtata agagttgaag atgaaatgtt
    13081 cacaggagat actttgtttg ctggaagtat tggtagaact gatttatata gtggggattt
    13141 taaccaaatg gaaaaatcac ttaagaaatt atgcaaatat gaagatgctg ttagagtata
    13201 tcctggacat ggaccaacca gtactctagg ggttgaaaag aagacaaatc cttacatgta
    13261 attttatatt agtatttaaa aaagtataaa atatttaata actatattag tgagggggtt
    13321 cttctactgt taaatagaag gagttaagtt gatgttgtat gtttttttaa aaggacatga
    13381 ttataagtat gaagtagcag aacttataaa attgtttacc agtgaattta agtttaaaga
    13441 ctctaatgct aacaataata tagagtcaaa gcatttaata aatagattga tttatgagaa
    13501 tggtgtatta ttctcaagta ctgaatacta tgaaaatgga aacttaaagt atgagtcaat
    13561 acagaatata aaaaatatga atcttgaatt tttagatgga tttgatagta gcaaaacgat
    13621 tgaagaattt cttaatagcc tagatagtga aagtaagagg aactttaaaa agctgtgtaa
    13681 agaaaatatc aaaaaaagca tgtttttagt gcttaaacaa gtatttaatt cttatgtacc
    13741 atggggaatt ttgactggta ttagaccagt caaaattgtc cacaatttaa tggataaaaa
    13801 gctagatgat aatagcataa gaaccattct aaaagataat tattttataa tagatgagaa
    13861 aatagattta gctcttgaaa tagctaaaag agagcgatta tttatgtatc ctatagataa
    13921 aaataaaatt tctctttatg taagtattcc attttgtcca actagatgtg tatattgttc
    13981 atttccttca aattcattaa aacaatttgg acatttaaaa agagaatatg tttataaatt
    14041 gattgaagaa attaaggggt ttgccaaagt tattaaggag actaagaaag aagttgaaac
    14101 tctatatatt ggtggtggaa ctccaactac acttgatgag gaagaattgg atttgcttat
    14161 aaattcattg ttcaatgaat tagatttaag taaaattaaa gaatttactg tagaagcagg
    14221 tagacctgac actattacag aacaaaaact aagagtgtta aaaaaacaca atgttagtag
    14281 aattagtata aatcctcaaa ctatgaatga tgatacttta gtaaagattg gaagagagca
    14341 tagagtatct gatttagtag attgctttaa tatggctcgt agaatgggat ttgataatat
    14401 aaatatggat ataattttag gtcttataga tgaagattta aatatggtaa agaatacatt
    14461 ggaggagata aaaaaacttt ctcctgaaag ccttacagtt catacattag ccataaagag
    14521 agcatccaac ttaaatataa atatggatag gtataaagaa catctaacac aatatgaaga
    14581 aatggtaaaa atgatagatt tatcaatgaa atatgcaaaa gatatgggat taaatccata
    14641 ttatatgtac agacaaaaac atatgttggg aaatttagaa aatataggtt atgctaaaga
    14701 aggatatgag tgcatttata atatacagat tatggaagaa aaacaaagta actatgcatt
    14761 aggtgcaggt gctatatcga aatttgttta tgtagatgaa gatagaattg aaagagtaga
    14821 aaatgtaaaa aatgttgaac aatacattga aagagtagat gaaatgataa aaaggaagaa
    14881 agaagaggta tataaaaatg ttgactaaag ctcctagagg aactaaagac ataactccaa
    14941 aagaggctta caaatggcgt tatgtagaaa ataaatttag agaaatatgt gctttatatg
    15001 gatatgaaga aatggtaaca cctatatttg aacatacaga acttttcaag agaagtgttg
    15061 gagatactac agatatagtt caaaaagaaa tgtattcttt taaagataaa ggtgatagag
    15121 aaattaccct aaagccagaa ggtacagcag gtgtagtaag agcatttata gaaaataaat
    15181 tatatgcaga tactcaacca acaaaattat tttatgtgac accttgtttt agatatgaaa
    15241 gaccacaagc tggtagacaa agacaatttc accaatttgg aatagaagca cttggaagtg
    15301 atactccttc aatggatgct gaaataatcg cactagctgt tcaatttttt aatgaagttg
    15361 gattaaatga tttggtagtg agtataaatt cagttggctg tccagtttgt agaaaagaat
    15421 acaatgcttt attaaaagag tatcttgact caaaagctga tatattgtgt gatacttgca
    15481 atgagagaag agaaaaaaat ccaatgaggg ttatagactg taaaaatcct acatgtaaag
    15541 aaaatatcaa agatatacca tttattgcag accatttgtg tgatgattgt aaatctcact
    15601 ttgataaact tcaagaatac ttaaaggaaa tgaatataaa ctttgtaatt gataaaacaa
    15661 tagttagagg cttagattac tatagaaaaa ctgcttttga aattatatct aatgatatag
    15721 gagctcaaag tactgtatgt ggtggaggaa gatatgatgg tcttgttgaa caattaggag
    15781 gaccaaaagg tataagtgga attgggtttg gactaggaat tgaaagactt ttacttacac
    15841 ttgaaggtaa tggaattgaa atagaaaacc ctcaatctac ggatatattt atagtaacta
    15901 taggtgaaga agcaaataca agaagcttta aattgttaaa agacttgaga caaaatcata
    15961 taagtgcaga taaagaccat atagagagaa gtgttaaagc acaatttaaa tattcagata
    16021 aaataaattc aaaatttaca atagtaattg gagatgatga acttaaaaat gacactgcga
    16081 cattaaaaaa tatgaaaaca tcagagcaaa ctactgtaaa attaagtaca ttagttgaag
    16141 aattaaagca aaagctgtaa aataagaaag tatgatgttt tgtgtattag ataaatatat
    16201 agcattaaaa ttaaataaaa tggaggtagt atcttggaaa ctttaaaggg tttaaagaga
    16261 actcactact gtggagagtt gagagaaaaa aacataaatg aagaagttgt acttatggga
    16321 tgggtacaga aaaaaagaaa tttaggtggt cttgtatttg ttgatttaag agatacaagt
    16381 ggattatgtc aaatagtttt tgatacagat gtagataaag aagcttttga aaaagctgaa
    16441 aaattagggg ctgaatttgt aatagctgta aaaggaaaag tatgcgaaag acaatctaaa
    16501 aaccctaata tgccaactgg tgatatagaa atatttgcaa ctgaacttag acttttaaac
    16561 aagtcagaaa ctccgcctat ttatataaaa gatgatgatg atgtttctga agcactaaga
    16621 ttaaaatata gatacttaga tttaagaaaa ccatctatgc aaagaaattt aaaattaaga
    16681 cataaagtaa tgaatattac aagaaattac ttatcaaaca atagattttg cgaaatagaa
    16741 acaccatttt taatagctcc aacaccagaa ggagcaagag attatctagt accaagtaga
    16801 gttaatccag gaaaatttta cgcattacca caatcaccac aattatataa acaattattg
    16861 atggtaagtg gtatggatag atatttccaa atagttaaat gctttagaga tgaagactta
    16921 agagcagata gacaaccaga gtttacacaa atagactgcg aaatgtcttt tgttgagcaa
    16981 gaagatgtta tgagtatgat tgaaggtttg cttgaagcga tatttaaaga agttttagat
    17041 gtagaattgg cacttccact tcctaagatg acttatgcag aagctatgtc taaatatggt
    17101 tcagataaac cagatactag atttggatat gagttaacag atatatctga tgttgtttgt
    17161 aactgtggat ttaaagtgtt tgctgatgct acacaaccag gaaaaagcgt tagaggtata
    17221 aacgtaaaag gcaaagctga cgactttact agaaaacaaa tatcatcact agaagaacat
    17281 gctaagacat atagagcaaa aggtcttgct tggatgaaag taggacaaga aggagtaact
    17341 tctccaatag caaaattctt taatgaagaa gaaatgaacg ctatacttac aagaatgaat
    17401 gctgaagtag gagatttact tttatttgta gctgataaaa actcaatagt atttgatgct
    17461 ttaggacaag ttagacttga agtagcaaat aggcttaatc tattggataa aaatgtatat
    17521 aacttactat gggtaactga gttcccagtt tttgaagaag atgaagaaac aggcacattc
    17581 tcagcaatgc atcacccatt tacatcacct atggatgaag atttagacaa gttagaagaa
    17641 ggtgataagt catctttaag agcgaaagct tatgatattg tattgaatgg ttatgaaata
    17701 ggtggaggaa gtgttagaat atcaaattct gatgttcaat caagaatgtt taaagcactt
    17761 ggttttacag aggaaagagc taatgaaaag tttggttatt tattagaagc atttaaatat
    17821 ggtactcctc ctcatgctgg tctagctttt ggtcttgata gacttgttat gttacttgca
    17881 ggaactgata atataagaga agttattgca ttccctaaaa atcaaaatgc tgtttgccct
    17941 atgacaaatg ctcctacatt ggcggaagat gagcaattag aagaattaag tataaaagtt
    18001 gatataaaag ataatgaata atatgtagtt gcaagaaact aaaatgtaaa aagtctggat
    18061 ataaagtata tttatattca gacttttttt attagaatta ttttatattt tttctctata
    18121 tattttcata aattatactt gtattctcaa ccaatataat taaaatacta tgtctaaaaa
    18181 ttttacataa acttggttgt tttagaaact acttctgtaa tgagtacacg attttaatta
    18241 aagaatatat aacaaatttg aaataagtct aatatatgac ttaatagatt aaattaaaat
    18301 aataaatttg gactatttat ctatttataa cttaaattat atcaaattga taacaaacta
    18361 gatattatac ttttttgtag aatagaatta caattatata aagaaaaatt ctattaatag
    18421 tgaaaaaaaa gtagaaaaaa gtagaaatta ttcttttata aagaaaggga gggataatat
    18481 tgcaagagag agaagaatta gttttaaatc cagttccaaa agaggaaaga gttggatggt
    18541 tagctccatt gtttaatatg cttggttcta acatagctat atctgaacta atggttgggg
    18601 gaactttaat tttaggaatg actctttcta atatgattat tacatcaatt atagggaact
    18661 taatattagt tgcaattatt atgatacaag gttatatagg ttacaaagaa gggttaaata
    18721 cgtatgtatt agctaagggg gcatttggag agataggagg aaagtatctt atatctctat
    18781 tacttggtat aactagtttt ggatggtttg gtgtacaagc aggggttgca ggactatcag
    18841 tacaaaagat attcccaagt gtaaacctaa cattagttac agttatactt ggattattta
    18901 tggttgtttt tgcactgtat ggttttaaag ctatggctaa atttaattac ttagttatac
    18961 caccacttat gatattaatg gtatggggag tttttaaagc tttttctact tatggagttg
    19021 aagctattta taattatact cctcaaacaa ctatgagtat ggttgaaggt cttaatattg
    19081 tagtaggtct tgttatagtt ggtgcaatta tatcacctga ccagttaaga tatacgagac
    19141 gagtaaaaga tatttggatt atcagttttt taggtctagg gattatttca ttattccaac
    19201 aagttgcagc tggtgtaatg tctatgggag cacctacttg ggatataaca gaagtattgg
    19261 ctaatttagg ttttggatgg gtagcgtttg ttattttaat acttgcatct tggtcaacaa
    19321 acgtatcaaa tgcttattct ggtggattag cattaaaaac tatttttcca aatgtaaaaa
    19381 gaaatgtttt gactttagtt gcaggtctaa taggtacaat aatagcagct actggtataa
    19441 tatttaaatt tcagtcattt ttatctttct tgggaatcgc agttcctgca atagcaggaa
    19501 ttatgtggtg tgagtattat tttatacaag gaagaactta taagcataga gaaggaataa
    19561 attggattgc agtaataagc tggttaatag gttttgcagc gtcgtattat agttcaaaaa
    19621 taaatttctt aatacctccg ataaatggaa ttgtagtgag tatggttatt tattatatat
    19681 taatgaaatg ttttggaatc aaagataaat aaagggggca attacatggg tcgtaaggtg
    19741 aaaataggta taattcaaca acacagtgta cttggagatg ttaaaaaaaa tatagaaaag
    19801 gcagtagaga tgatagatga cttaggaaaa caaggggcag atataatttg tttaccagaa
    19861 ctatttgcta caggatataa tttagagtct ttaggtggag ttaaaacatt agaattaata
    19921 agagaacata ataagtacat agaggaatca atgtcagaag ctgctaaaag aaataatgta
    19981 tacctgatat ctccatatgg aaccttagaa aaagggtcta cacatgttta taattcagca
    20041 gttatatttg atagaaaagg taagattatg ggagaatact gtaaaaatca tctgtggtca
    20101 ttagaagcag tatactttaa aggaggggaa aaagtagagg tatatgatgc tgattttggt
    20161 agatttggtg taatgatatg ttatgatgca ggttttccag aagtatccag agaattgaca
    20221 ttaaaaggtt ctgaaataat atttatacca tctgcatgga gaatacaaga tgaagatatg
    20281 tgggatttaa atgtatctca aagagcttta gagaacactg tatatacagt aggtgtaaat
    20341 ttagtaagta atgactctaa tttaatacta tttggaaaga gtaaaatatg caatcctaga
    20401 ggtactgtaa taactcaatt agatacatac agggaaaaat atgtattatc agagattgat
    20461 ttagatgaaa taaaaaagta tagggaagat atcccctatt tgaaagacag aaaaagtagc
    20521 tataacatat aattctatac tataatacaa gttcaatata aaatgataaa aagaggatgt
    20581 atcaatatta agattaaatt tattatactt tgatatatcc tctttaatat tatcaacttt
    20641 gatttaaata tttttaaaat agttgataag aaagtttttg aacaacaaca cagctttaga
    20701 ttcatagttt tcgctaaacc atgaaagatt taaatatctt ttaaattcaa tatctttgat
    20761 atgtaacaac ttaatctcag ggtcttgaga taatccccat gattttccac atataaatcc
    20821 aactccttga agtgatttta taagagcata aattgtatat ggtgaatcag actcaaatgc
    20881 tattttgggt ttgaaattag ctgattcaca caggatatca attacctctc tataattctc
    20941 acccttcgtt ataactataa agttttcatc tgaaacttcg cttagatata cttcatcctt
    21001 taatgataaa ggatgattta ttgaaactcc taatagtatt tcttcgcatg ttaaagttat
    21061 gctattttct gagtttagct ttgtaaatga agaagatatg tacaaatcaa aatcactttt
    21121 tttataagat gatggcaaag tatgtgacac attaaaagta atattgggat atagttttct
    21181 gaaattactc aaaagtgatg gtaacacatt agccgctgca ggagataata tctttacttc
    21241 tcctgaattt tctaaagaag tgtcttgtaa ctcctttttt gaactttcta ctaaatctaa
    21301 aatgctatct actcttttta gaaacaatct tccatactca tttaaaacaa tatatcttcc
    21361 ttttctgtca aataactgaa ctccaagttc tttttctaag gaagatatag ttttgcttaa
    21421 agctggctgt gctatattta acttgtgtga tgcttttgtt atgtgctcca ttctagcaac
    21481 agtttgaaaa tacttaagat gcaataaatc catagtataa aatacacctc ttttaatatg
    21541 acttgagtta tatcaatttt ataatcaact ccacattata cattataata taaagtttat
    21601 ataataaact acaatatatt aaaaagttat ttaaattgtt atttatattt tgtacataat
    21661 ctaattaaaa aaagtaactt taaa
//
LOCUS       DAKBNN010000046        21661 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000046 DAKBNN010000000
VERSION     DAKBNN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.046"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            48..638
                     /locus_tag="OUB02_001526"
     CDS             48..638
                     /locus_tag="OUB02_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8885289.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            752..1597
                     /locus_tag="OUB02_001527"
     CDS             752..1597
                     /locus_tag="OUB02_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885290.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKLKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTAECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            1594..2277
                     /locus_tag="OUB02_001528"
     CDS             1594..2277
                     /locus_tag="OUB02_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885291.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IVIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            2274..2990
                     /locus_tag="OUB02_001529"
     CDS             2274..2990
                     /locus_tag="OUB02_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885292.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMLGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            3087..3350
                     /locus_tag="OUB02_001530"
     CDS             3087..3350
                     /locus_tag="OUB02_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885293.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            3322..3777
                     /gene="def"
                     /locus_tag="OUB02_001531"
     CDS             3322..3777
                     /gene="def"
                     /locus_tag="OUB02_001531"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCT8885294.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            3829..4290
                     /locus_tag="OUB02_001532"
     CDS             3829..4290
                     /locus_tag="OUB02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HCT8885295.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            4340..5242
                     /locus_tag="OUB02_001533"
     CDS             4340..5242
                     /locus_tag="OUB02_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8885296.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            5612..6424
                     /locus_tag="OUB02_001534"
     CDS             5612..6424
                     /locus_tag="OUB02_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8885297.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWETNEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            7471..8232
                     /locus_tag="OUB02_001535"
     CDS             7471..8232
                     /locus_tag="OUB02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HCT8885298.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            8297..9079
                     /locus_tag="OUB02_001536"
     CDS             8297..9079
                     /locus_tag="OUB02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCT8885299.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            9346..9765
                     /locus_tag="OUB02_001537"
     CDS             9346..9765
                     /locus_tag="OUB02_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HCT8885300.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(9868..10134)
                     /locus_tag="OUB02_001538"
     CDS             complement(9868..10134)
                     /locus_tag="OUB02_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCT8885301.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            10361..11062
                     /locus_tag="OUB02_001539"
     CDS             10361..11062
                     /locus_tag="OUB02_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885302.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            11144..11752
                     /locus_tag="OUB02_001540"
     CDS             11144..11752
                     /locus_tag="OUB02_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT8885303.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            12076..12540
                     /locus_tag="OUB02_001541"
     CDS             12076..12540
                     /locus_tag="OUB02_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HCT8885304.1"
                     /translation="MKKLVISLISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNACLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNIWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            13177..14193
                     /gene="gap"
                     /locus_tag="OUB02_001542"
     CDS             13177..14193
                     /gene="gap"
                     /locus_tag="OUB02_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCT8885305.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            14588..15757
                     /locus_tag="OUB02_001543"
     CDS             14588..15757
                     /locus_tag="OUB02_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885306.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            16138..20391
                     /locus_tag="OUB02_001544"
     CDS             16138..20391
                     /locus_tag="OUB02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HCT8885307.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATEEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            20540..21034
                     /locus_tag="OUB02_001545"
     CDS             20540..21034
                     /locus_tag="OUB02_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCT8885308.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            21150..21527
                     /locus_tag="OUB02_001546"
     CDS             21150..21527
                     /locus_tag="OUB02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCT8885309.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         8738 a         2100 c         3719 g         7104 t
ORIGIN      
        1 tattatattg actatataaa ttattttagt caaactggag gaaaaagatg cctaaatcat
       61 attctgataa agaaagagag tatataataa aaagacttaa agaagaagca agattgtgta
      121 tggagcaata tggtattaga aaaactactg ttgatgagct tgtaaaacgg gtaaaaatac
      181 caaaaggaac tttttatcta ttttttcaat caaaagaatt gctctttttt gaggtattaa
      241 gagatataca tgactctata caaaaagaaa tcttatatga gataaataga gtagatgaaa
      301 gtattacatg tgaacaattg acagatatat ttatgaaatt ctataggatg gttgatagta
      361 cttcaatttt aaatctcatg ataaatggag aatttgagat attggtgaga aagttaccag
      421 attctataat tgaagaacac tttagacatg atgactttga aattggagag attatatctt
      481 gcataccaaa tgcaaaaaat aaagacatag aaagctttag tggagcattt agagcagttt
      541 ttcttaccat gctatataag cgcgaggttg gaagtaattg ttttgaagat gctttaagat
      601 taatgataaa cggattagtt atacaactta tggaatagaa tttaaatttt gtttgctaat
      661 aacttttatt aaaaattatt aacaaacaaa aaaattatat tttattgacc atatagtcaa
      721 aatagtcaaa aatttgaaag ggtgatattg aatgattaaa gttgatgatt tatcatttag
      781 ttatacagat agagattttt tacaaaatat taactttgga gtaggaaaag gggaaatact
      841 aggattttta ggaccatcag gtgcaggaaa atctacatta caaaagattc taattggaat
      901 gataactaat tatggaggaa gtgtcattgt aaatggagta gagagtaaaa gacactcaaa
      961 taaattttat gaaaatatag gagttgattt tgaatttcct agtttatatg aaaagttgac
     1021 tgcaatagaa aatttaaaat attttgggtc tctatattct aaaaaactac tatctattga
     1081 tgagttatta aaatcagtag gactagaaaa tgaagcaaat aaaagggtat ctgagtattc
     1141 aaaaggtatg aaatctcgat taaattttat aaaagcttta ttacataatc cagatatttt
     1201 atttttagat gaaccaacaa gtggtcttga cccatcaaat agcaaggtta tgaaagatat
     1261 aattttatca gaaaaattga aaggtaaaac tattatttta acaactcata acatgttaga
     1321 tgcaacagag ctttgtgaca gagtagcgtt cattgtaaat ggtaaaatat ctgctttaga
     1381 tacaccacat aatcttatta tgtcaaaagg agctattaaa gttagatata cttactttga
     1441 taatggagaa aaaactgctg aatgttttct gaacaatact gctaatgata aaaatttgaa
     1501 tatgcttatt gagaaaaata aacttttatc aatacatagt agtgaaccaa cattaaatga
     1561 tatttttatt gagataacag ggaggaattt acaatgaggc taaaaaatct tattttagga
     1621 gatataaagt ttcaatttaa gtatggtttt tattttttat atcttttttt aagtattata
     1681 tatatttgta ttatcaatgt atttccaact tttatgagag aaaaaatagt tataattatg
     1741 atttattctg acccagcagc aatgggattg ttttttatgg gagctattgt acttcttgag
     1801 aaaagtcaaa gggttttaaa ttccttagct gtatctccag taaaagtatc tgagtatatt
     1861 ttatcaaagg taatttcatt aggagtaata tctagtattg tagctatgtt tattgctatt
     1921 acattaaatt tagataatat tataatatca actataggta cctttttttc ttctattata
     1981 ttttctttgc ttggattgat acttgcaagt aaagcatcta gtttgaatca gtttattgtt
     2041 ttaagtattc ctattgagat tatatgcttt attccaccaa tactcaatgt acttcttgac
     2101 acaaaatcat atgcaaattt gtaccctttt aatatatgta tttctcttat tagtggagat
     2161 aaaaatttta taatgattaa tattttaatt ttaatatcaa ttataataat aatttatttt
     2221 ataacttatt attttatttg tagcagttgg aaaaaggtag gtggagtaaa actatgataa
     2281 agattataaa tgcgtttaaa caagagtttg aacaaattaa gagagatgct atgcttttta
     2341 tagtatgtgt ttctccaata ttatgtggag tatttataaa atttggaata ccattaatac
     2401 agaatatatc acttaacaag ttttattatc aattaaattt agagccgtat ttcttgatgt
     2461 ttgatttact tttagctttt attacacctt ttatgttctt ttttgcttct acaatggtca
     2521 tattgggaga aattgatgac agcatttcaa gatatttaat agtaactccg cttggtaaaa
     2581 ctggttatct tatttcacga tttggaatac caggtatctt agcatttatt attactatga
     2641 ttctacttat ctttttttct ctaactaaaa tttctttctt gttaaactta tcaatttctt
     2701 tattagtttt attacaagga attattatat caatattagt gatttcttta tcatcaaata
     2761 aactagaagg aatggtaatt acgaaattct caggtgtttt tatgctgggt attttagcac
     2821 cattttttat tttaaataaa gcacaatata ttctgttctt cttaccaact ttttggatat
     2881 cgaaggcatt taaggaagat aattatatct atatgtttat ttcaattgtt atatcattaa
     2941 tatggatttt attattattt aaaaagttta gtaaaaaaat agttaaataa gaaaatttaa
     3001 tatataaatg taaggttgac ttgacagtaa tgttgaaaaa tgttacattt aagatgtaaa
     3061 gttaacctta catgaatgga gcaaatatga aaaataaaat aaagatatta agagaaaaat
     3121 taggattgac acaagaacaa ctagggagat tagttggaac atctagacag gctataaatg
     3181 ctatagaaac tggtaaaaat gaaccttcaa tatggttggc atatgatatt tcaagaattt
     3241 ttaatgaacc aattgaaagt gtatttttat ttgaagaaag tgaaagaaaa tcaagagcac
     3301 agataagtag gggagaatat tatggctata agaaagataa gaacatttga tgatgaaatt
     3361 ttaagaaaaa agagtaagta tgtagaaaac gtagataata agataagaga aatattaaat
     3421 gacatggcag agaccatgta caatactcca aatggaggag gattagctgc atgccaagta
     3481 ggagtattaa agcgattagt tgttatagat ttaggagaag gacttattaa gcttgttaat
     3541 cctgaaatta ttaaacaaga aggggaacaa attgttgttg aaggatgttt aagttttcct
     3601 gaagtatggg gtaaattaaa aagaccaaaa aaagttacag tacaagctct aaacgaatac
     3661 ggtgagaaaa tagaaattaa aggttcaggt tttatggcaa aatgtctttg tcatgagata
     3721 gaccatttaa atggtatagt ttttacagat aaaattattg aacatgtaaa attgtaagat
     3781 agattggtta ttgaatttat aaaatatttt atagtaagga gttaaattat gagattatgg
     3841 cataaggatt taattgatgt attacctaaa aatcaattag taagtcaatg gagagagcta
     3901 cttgctataa aaggttctat agataaaaaa ggtactccaa accatctatt agtgaataaa
     3961 gtattgaatt atagtataga tgaatttaaa ttttatacaa aaattgtaca tgatgaaatg
     4021 ttaaaaagaa attataaacc taatgaactt aaatatacaa gtatattaaa atggaaaaat
     4081 agaaattttg caaatgatat atctaatgaa catagtttaa atttagaaaa tttatatgat
     4141 gattggcata ataaaatgta tttaaagcaa tgcttatata atttagaaga aaaagcaaca
     4201 tgtggaggaa taccaataaa tgaatggaat atattacttt gtaaatatag taaagattat
     4261 gaattgtggt ctggaaatat catgttttaa ttactataga ttaacattat agtattattg
     4321 tatataaaga ggtgattatt tgtataataa aataaaatat attccaataa attgtgaaat
     4381 agcttgtaat aaattaaaaa ggagtatgcc atataaatgg gatttaaata tttatcgtgg
     4441 atgtgagcat ggatgtaaat attgttatgc tatttactct cataagtaca tcaactccaa
     4501 taattatttt gaagagattt atgtaaaaac aaatatagta gaaaaacttg agagacaact
     4561 taaaagcagt aaatggaaga aagaagtaat caatatagga ggagttacag atagttatca
     4621 accaatagaa gctgactata aaattatgcc agaaatatta aacctgctga taaaatacaa
     4681 gacaccagct attatttcta caaagtcaga tttaatttta agagattacg atttaattga
     4741 taagctatct aggattacat atatcaatgt agcatctaca attaccacag taaatgaaga
     4801 tacacaaagg cttattgagc caaatggtgt ggattcaatg agacgattta aaatgttaaa
     4861 agaatttaga aaaactaacg catcagttgg tctacatatt atgccaatta taccatatat
     4921 tacagatgat tttgataata taaattcgct atttagacat gcaaaagaat gtaatgtaca
     4981 ttatgtttta cctgggactt tatacttaag gggtataact agaggtgttt tttttgactt
     5041 tgttaaaaaa gagtttcctg aattatttga taaattatca atactatata gtgcaggttc
     5101 acttaatagg gagtataaaa atcaattgta taaaatggtt aatgaactta gatataagta
     5161 ttctctatca agcagttact ctaaaataat gaaggaaaag ttaaaaaact caggagatgt
     5221 acagatatct ttttttgatt agttttatat aaaaaaaatt tttttactat gatgattaaa
     5281 atttattaaa attaaaattt attgacaaat ataaaattta aatatataat taattcataa
     5341 aataaaaata tgaaaggatg atgttgttcg gtgatatttg atatcataaa atcgttgata
     5401 taagtattgc tggaaaagag tgaataaaat caaatttaat aagcaaacaa agtaaagcta
     5461 tagttttatt gttgtgctta aaaatatttg gttctatttt actcttattt aaactcatct
     5521 ttttgaagtt aaaccacaga gtaaactctg tggttttttt attttaaaaa taaattttaa
     5581 ggagtggtat gttttgttaa acaaattatg tatgtattta gaaagaccag agctttataa
     5641 acaaagtgaa attaattttt gggatgatga gtatatttca aagcaattat taaaagcaca
     5701 cttagacact aattttgaag gagcaagtag aaattttaac tttatcgagg attctgtaaa
     5761 ttggatagtt acagttgcaa atccagctaa ttatcctaaa ttacttgatt taggttgtgg
     5821 tccaggttta tatgcagaga aatttgcaca aaaaggttat aaggtaactg gaattgattt
     5881 ctcaaaacgt tctattaact atgcacaaaa tagaaataag gagactaatt taaacataaa
     5941 ttacttgttt caaagttatt taaatatgaa ctataatgag gaatttgatt tggctacctt
     6001 aatttattgt gattatggag ctttgtcaac tgaaaataga aggttattaa tggaaaaaat
     6061 ttatgatagt ttaaaacctg gaggaaaatt aatattagat gtatttacaa taaataaata
     6121 caataatttt gaggaaatta aatattggga aacaaatgaa gatggaggat tttggagtaa
     6181 tgaaaaatat atgtgtctac aagataattg taaatacaat gattataata cattagaaca
     6241 aactcttgta ataaccgaaa aagatgaaaa tgtattttat gtttggaatc actatttttc
     6301 aaaagaaagt ttcttattag aagttgaaaa tattggattt aagtctgttg agttttttag
     6361 taatgttaag ggtgaagcat attctgacga tagtatgact atgggattag ttttacaaaa
     6421 ataataattt ttttaaaatg aataaataaa aacttgaaac ctttaacaat aaaataacat
     6481 aataaaagct tacatattga aaaatggaaa cttttatgtt atttttagta cattacaaat
     6541 ttgcttatat aaaaatcaat acttgaatta ttatttttat gtgaaattta tctatagtat
     6601 atttttataa acaagtaatg ttattaaaat taagtatata gttaaaagtg tatagcaaaa
     6661 aggctgttat attaatgtta gaggttcagg gaaaatatat aaagcaaagt atttacagat
     6721 aatgtagatt gcacagctat aagttagata atagaattat gtaatcaaga atttacaaca
     6781 aagtataaga taaggataat gctaaattgc catacaggtg ctagatgtgt aaatggattt
     6841 aattaatact attgataaag tgcaacaagc acataaacca attgatgaga ttattaaaac
     6901 tataagagat atagttgaca taattaaaat attggaacct atttataatt ttaaatcaaa
     6961 tcaataatga ttaagattaa aataatatta aggcatcaat gttataagag tacaatataa
     7021 ggagtactat atagtactcc ttacttatac atattaggca ataggaaatt tttcatagtt
     7081 aaattttcaa atatttttta gggaacttta taattgagaa ttaagggtat agtatgtgat
     7141 gaacttacta aatttataat taggttatat aatggaataa tcatcaatag ttttacttta
     7201 gggaaaatat atgcttaagg aagtatagct tactaatatt attaaaatgt atatttaaaa
     7261 tgataaaaaa agaattagac aagtatttag taaattgact atgcaaaatt ctatcaagca
     7321 ggaacactat gactttagtc atgggaggtt cagtaggtaa cttgttgaat taagtttata
     7381 ttatttaaat aaactactta atttagataa tataaaaagt cagaatttta agaatagagc
     7441 tatataatct tagctattag gagaagtact atgataggaa tatattttag tggaacagga
     7501 aattcaagat actgtgtaga aaaattttta caagaatatg atataacagc aaattcttat
     7561 tctattgaag ataatgaact tttacagcac atcaataatc atgagaatat tatttttagt
     7621 tatccggtgc aatatagtaa tgtgccaaaa atattgaaag attttataat aaatcattca
     7681 actttgtgga atggtaaacg tgtttttatt attgcaacta tggctctatt tagtggagat
     7741 ggctctggtg tgttagctag aatattaaaa aaatacggtg ctataactgt tggtggatta
     7801 catgtgaaga tgccagatag tataggagat gaaaaggtat taaaacattc tttagagtat
     7861 aatagaaagc ttgtaataaa agcagaaaag aaagtaacta aggctgctaa aaaactgaaa
     7921 catgggtcac caccaaaaga agggttgggt tttttgagtc atattgtagg gttatttgga
     7981 caacgattgt attttatcaa taaaacaaat atgtatacag ataaactaaa aattaatact
     8041 gaggaatgta ttgtatgtgg taaatgcgtt aatttatgtc caatgaagaa tctagtaatt
     8101 aaaaattgta tgattgttgc aaatgggcaa tgtacaatgt gttatcgttg tgtaaataat
     8161 tgtgccaaaa aagcaattac attgttaggt aaaaaagttg ttgaacagag taatattaag
     8221 aaatatatgt aagaaattta aagttttatt gaatgtaaat tgatgtatat tttatgttag
     8281 aaagagaggt taaataatgg aatactacat agttgattca tttgcaacta aactatttaa
     8341 aggaaatcct gctggagttt gtgttcttga cagaagaata cctttagagt taatgcagaa
     8401 aatagcagaa gaaaacaatt taccagaaac agcatttgta gttaaaggta aaggaaatta
     8461 tgaattacga tggtttactc ctaaagcaga aattgattta tgtggtcatg caactttagc
     8521 agcagcttat gtaatatcaa attttattga tgttaatgta aaaaggatag atttctttac
     8581 tcaaagtgga aagcttgaag ttacacgaaa tggaaatcta tatgaaatga tatttccaga
     8641 aataatgcct attgaaatag agttatcacc acaacaagct aatttgatag gctgtgtacc
     8701 aagtgctgtt tattcttcaa gagatttaat cttattgcta aacagtgaac aagaagttat
     8761 aaattataaa cctaattacg cacaattgcg taaattgaca gactggttag gtataataat
     8821 cacagcacaa ggaagtaata cagattttgt atcaagatat ttttgtccag aattagattc
     8881 agaagaccct gttactggtt catcacattg taatcttatt ccatattggt ctgaaaagtt
     8941 gggaaaacac aaaatggtag ctgctcaact ttctaatcgt ggtggtataa ttcagtgtga
     9001 agtgttgaag gataatactg taaaaatttc tggtgaagca gtcttgttta tgcagggaac
     9061 aataaagatt gatatttaaa taagtataat aactaattta ttatgaaaaa taattaattt
     9121 tcttagtgtt aacaataata aagtagagtt aaaaaacttg atttcactta aatcaatatg
     9181 atagatgttg atactaagat tgacaagcat gattaaaaaa gataatattt aatctataaa
     9241 gataaaaaca cagtctggtt ggtagtccag acgcagcaat agctgtcagt aacctgcctc
     9301 cttggttgtc cgttctttag aaagtaattt aaggaggaac tagcaatgga caaaattagt
     9361 ggaaaattaa cagtcttatt tgaagaacca ttttgggtag gtatatttga aagacaagat
     9421 ggtaaaaaat atgaagcatg tagggtagtt tttggagcag aacctaaaga agtagaagtg
     9481 tatgaattta tacttgaaag atttttctca ttagattttg gtagcattaa attagaaaaa
     9541 aatgtgacca aagacaacat tggatacaaa agaatgcaga gaaaagtaaa aaaggaacaa
     9601 gaaaaagaaa ctatagggac taaggctcaa aatgctctta agcttcaata tgaagaaaga
     9661 aaacaggata gaaaaaacct agctaaaaat agaaaagaag aagaaaaaga aagactattt
     9721 aatttgaaac aagaaaagag aaaagctaaa cataagggtc attaatttta attaaatatc
     9781 tttttaataa ttgctagaaa aaggtgtata aaatttttaa ttttatacac cttaatttat
     9841 tgaaaagaat aaggttttat atttcaatta ctttaaaata taaaaatata ctaataaaaa
     9901 ttgtaaaaat agtataaatg gtataccaaa tgtaaattta atgtgtttag ttttatgtct
     9961 aaaagaatac attccaatta tagaacctat actacctcca attactgcta tagacataag
    10021 agtagcttct tttattctcc attcgtttct aattgctctt ttcttatcta tgtacattga
    10081 aaagaaagca atagaattaa tcactataaa atatattaga ataaaatttt tcacagtctt
    10141 gcctccataa aaatatatta tacattataa aacaagatat ttgttttgga aagttaaatt
    10201 tgtaattagt acatataaaa tatttgataa gaataaatat aatattaatt ggaaaattta
    10261 aaaaaattaa acaattagca aatagggggt atatttataa gacacaaagt gataaaatct
    10321 atttagaaaa taatgtaaaa taacaggagg ataaagcaaa atgaaattta tagtagaaaa
    10381 agaagttttt gataaattag aaaatgtatg ctttggtgta gtagttgcaa aaggaataga
    10441 taatactaaa gaaatagaaa gaataagcaa ccttttagat ataagtatag atagagtaga
    10501 agattacttc aaagataaaa aagtaaagga atcagaagaa attataccat atagagaggc
    10561 atttagaagt ctaggcatga acccaaataa atttatgagt tcgatagaag caatgacaac
    10621 aagggttgct aagaataaga aacttcctca tataaatcct attgtagatt taggaaactc
    10681 tatttcttta aaatatttac ttccaatggg cgctcatgat atggatttta gaaatgatga
    10741 tgtatatgtt agattttcta aaaaagggga taaatttgta ccatttggag aaactgatgt
    10801 tgaattaatg gaagaaggag aattaattta ttcagttgga gacatggtaa aaactagaag
    10861 atggatatgg agacaaggag aggaaggaaa aattactaat agctcaaaaa atatattttt
    10921 tcctatagat ggttttactg atgctaattt agataaggta atgagtgcaa gagaagaatt
    10981 agcaaaattg ttaaaggaaa tatttaattg tgaaattaaa gtaggttttg tagataaaga
    11041 taatcctgaa atggaaatat aataagttgt tgaatacttg ttaagatatt atatttactt
    11101 aataatgtta aaaaggtatt aaaaaagaaa ggtcgtagta aatttgaaaa aaactataat
    11161 aaagaatgta aatccaatga ttgaaaataa gtttatagga ctttttgaaa tagagtataa
    11221 aaacaaatta gatgaagata aggtttggat ggtagcatca agaaaaagta gtgagcaatt
    11281 aaaaaatatt tatttgaaga atgaagaaga tagtgtagat gcagttgtaa tagttgggct
    11341 tcataaaagt agtaaaaaat taattttaat aagacaattt agagtaccta ttaatggata
    11401 tatatacgag ttaccagcag gtcttgttga tgaaggagag tctatagata tttccgttga
    11461 aagagaactt agagaagaaa caggacttac tcttttagaa attaaaaaaa ataaaagtat
    11521 tgataaagtt tatttatctc ctggaatgag tgatgaatct gttgcctttg tttattgtat
    11581 atgtgatggt gatataacag atgaattttt agaaccagat gaagagattg aagcgctttt
    11641 aatttcgcaa gaagatgcaa aagagatttt acaaagtaat cataagatag acacaaaagc
    11701 atttttgatt ttacaaatgt ttgtgagttt aggagctaaa ttatttgaat agatatatag
    11761 aaaagaggtt atatagaaga gtaaaataat aaagtttatt ttacttgtat aaattataat
    11821 ctcttttttt atacaataaa gtataatttt gtgtattttt aaataagtga cttgactctt
    11881 aaattttcat aatatgttat aaaccaattt aagatactac aataattaaa attccaaaat
    11941 tatttaacat atttgcaatt aaaatgagaa aaggtaaagg ctgaatgatt gaaaaatttg
    12001 atgtaaaata gtagattagt ttaatactat agtttgtagg gttaaaaatt agaaattgta
    12061 agggagataa attctatgaa aaaattagtt attagtttaa ttagtatcat tagtgtggtt
    12121 agcttgacag cttgtaaaaa aagcaatgaa agtaaatata ataatttaaa caacaaagaa
    12181 tcaggacaaa caaccattga aatggactta gataaaaatt atgatacttc agacccattt
    12241 gttaatgcat gtttgttttg tgtgtctaat gatatagatg ttttagacac agagatttct
    12301 tttaaaatgg atggagatag tggtattgtg gaaataaagg acaataaaac agatgaaact
    12361 ttatggagta atatatggca tggaagcgtt gataatgata cattcgcaat ttctctttct
    12421 aatatacaaa aagagaaaga gtatgctata tgctttactg gaattaaaat aaatcatgca
    12481 gttgtaaagg ttacttttga aagcaattta gttaaggaga aagaaaaacc ctcaaaataa
    12541 attccagatt gtctataact tgatattcag tacataaaag cagttgatat ttatgattga
    12601 ctgctttttc ttatcaaaaa ggataggttg tgcattttta cttgaaatag tttatgcaca
    12661 tcaaaagttg gtgagcatga tattggaaat ggtaagcatg aaatcttgat aacacaagga
    12721 aataaattta agttttcaat taagtatatt tccataattt agttaacaat aagctaatta
    12781 ttatttatat tttgatatat aaataataat tgtagcaaat tttaaaaaat catatatttt
    12841 ttgaactaaa taaaaattta tttttattat tttaaattag aggagattct tgaagatata
    12901 ttatttttaa aaataagtat agtttaaaaa ttcaaaaaaa agatggcaaa atatgtccca
    12961 agggacattg agtaaaataa atgatttcag cttctttaca ctaaaaagta catactaaat
    13021 gaggggtaaa ataaagaaaa atgatatact aattgtcttt tgataataaa aaaacatgga
    13081 atttgattat ttaatcaaga aaaagtattg aacaaatgat ttatttagtg tacaataatc
    13141 atgtaaaaac atagtaatgg agggagacat ttcataatga aaattaaggt aggaatgaac
    13201 ggatttggaa gaataggaag agctgtatta agaatagctc aagaagaatt aggagataat
    13261 atagaaattg tagccataaa tgcaagagca acaacagaat ctttggcaca tttatttaca
    13321 tatgattcat gctatggaac ttttagaggt gaagtagagg caaaggatga agacactcta
    13381 gtagtaaact ctaaagagat aaaaatatta agatataatg acccagaaga attaccttgg
    13441 aaagaattgg gggttgatat agtaatagaa tcaacaggat tatttacaca aagagaaaag
    13501 gcagaaaaac atattaaagc aggagcaaaa aaagtaatta taacagctcc aggaaaaaat
    13561 gaagatataa ctatagttat cggagttaat gagaaagaat atgataatga aaagcacaat
    13621 ataatttcaa atgcatcttg tactacaaat tgtctagctc cttttgctaa agtattggac
    13681 gaaaaatttg gtatagtaaa agggttaatg actacagtac attcatatac taacgatcaa
    13741 agaatattag ataaaagcca taaagatttg agaagagcaa gagcagcggc tgaatctata
    13801 atacctacca ctacaggagc agcaaaagct gtgtcaagag tactaccaca attagaagga
    13861 aaattaaatg gtttttcact tagagtacca acaccaacag tttcacttgt tgatttagtg
    13921 tgcgaactta aagaaaacgt tacatctgag caagttaact ctgtgttaaa agatgctgct
    13981 gaaggagaat taaaaggtgt tttaggatat tgtgacaagc cacttgtttc tatagattat
    14041 aaaggagatt caagatcatc tattatagac gctctatcca caatggttat agaagacaat
    14101 atggttaagg tagtttcttg gtatgataat gaatggggat attcttcaag aactgttgac
    14161 ttagtaaaat atgtagctga aaaattaaaa taatatatat ttcttaaagc aaattttaaa
    14221 aaaatttttt aaaaaaaata tataaaacta aaaaaaaaga ttatgaaaaa tattgaagga
    14281 aaaatttttt cataatcttt tttctttcat gcaaaaagat acaagttaca acttatattt
    14341 gcatattagg tcgttagaga agtatatata tatttagtaa ggaatcatac tttttataaa
    14401 atgaaagata agatgtcgaa aacttgcata aatctgaaaa atttttatta ttaaaatact
    14461 gatttccatt ttaaaaatga taaaaaaatg acaaataaag ttgcaaatag taatacttat
    14521 aagtataatt aaaaaagaga aaaggttttg ttaaacttta taacaaaaaa agaagggatg
    14581 attcaaaatg gagaagttaa cagacaggag acaggttaag gcagtaagca tggaaacctt
    14641 cattttccta atattactag tagttggttt tgggtatgtt ggtagtatta tgggagcagg
    14701 aatgatgttc aaagtaataa tgagtacagc acatgcttta ctattggaga cagttttctt
    14761 aattatggct atggcagttc ttgcaggcgc attaagcgca ctactttcag agtttggagt
    14821 aatagcgtta attaataaaa ttttcgctgt atttatgaag cctttatatg gactacctgg
    14881 tgctagtata gcaggagcta taacaactta tttatcagat aatccagcta ttataccttt
    14941 tgctaaagat aaaacattca cacagtattt taaacaatac caagttccag cattatgtaa
    15001 cttaggaaca gcctttggta tgggacttat cttaacaaca tttatgattt ctcaaggtac
    15061 agaatatgta cttccagcat taattggtaa tttaggtgca attataggta gtgtcataag
    15121 tgttagaata atgttgacat ttactaagaa gttttataaa tacaatccgg aagaagataa
    15181 agcaacagga acattagaga aaaaggatga atttagagaa atcagagaag gaaatgtatt
    15241 tcaaagagct ttagatgcaa ttcttgaagg tggtaaaatg ggggtagata tgggaatggc
    15301 tataattcct ggagtcctag tagtatgtac attggtgatg ttattaactt ttggaccatc
    15361 tacagaccct gttacaggtc aagaagttta tacaggagct gcatatgaag gaataaaatt
    15421 acttccagtt attggagata aacttggatt tatattagaa ccattatttg gatttacatc
    15481 accagaggca atagcattcc ctataacttc attgggagct gttggtgcag caatgtctct
    15541 agttccagag tttataaagt caggagctat aactcctaat gatatagcag tatttacagc
    15601 tatgggtatg tgttggagtg gatatttaag tacacatatt ggtatgatgg atgcattaaa
    15661 tgctagacag ttggcaggta aagctattct ttctcataca ataggtggtt tatgtgcagg
    15721 agcagctgct cactttatat ttacactcgt aggataaatg taatcagcta agataaaaaa
    15781 taatatacaa aaaaatggtt gttgatatgt aataagaatt tacatatcaa caaccatttt
    15841 tttctgacta aagataattt ataatacata aacattaagt ggatattaga gttaagttat
    15901 atttatgtaa aaagtaacaa aaatctgtag aaatttatga caaaatttag tacactaact
    15961 atatgttctt atttaaaatt taaggttaga attaggaatt acctagtaga gtaagctttg
    16021 attaataaaa gaatcataaa ataaaaatat tagtcataaa gattataaat aacttactta
    16081 aaataaagcc atagaactta caaaaattaa aaaagcatat aaatatggag gggtacaatg
    16141 gatgaattat ttcatatagg aattgatgta ggttctacaa cggtgaaggt tgtggtactt
    16201 aataatagta ataatatagt tcataaagaa tatagaagac attattctga tgtaaaaaaa
    16261 tcagttaaag aagtactaaa tggtatatat gaaaaattgg gagatataaa tactaccatt
    16321 ataataactg gttcaggtgg aattggaata tcaaaaaaat taggtgtaaa atttgttcag
    16381 gaagttatat ctagtacaaa atcaatagag tattttcatc ctgagactga tgttgtcata
    16441 gaacttgggg gtgaagatgc aaagataaca tatctaagtg gaggaataga ccaaagaatg
    16501 aatggaatat gtgcaggtgg cactggagca ttcatagacc agatggcatc acttttaaag
    16561 acagatgcca gtggattaaa tgaacttgct aaagggtata atgttatata tcccatagct
    16621 tcaagatgtg gtgtttttgc taaaacagat attcaacctc ttataaatga tggagccaat
    16681 cagactgata tagcaatgag tattttcaat gcagttgtcg tacaaactgt gagtgtcctt
    16741 tcttgtggaa gaaaaataga aggaaatgtt gcgtttttgg gtggtccatt gtacttttta
    16801 tctgaactta gagaagcatt taaaagagta ttggatttaa aagatgaaaa cataatcttc
    16861 ccagaaaatg cacaacttta tattgctatt ggcgcaggat tattatcagt agatgaagat
    16921 aatatacaac taaaatctct aatagaaaaa ttggatagca taaagaatat tgaagatgga
    16981 gaagtaaatt tattagaacc tttatttaaa gataaaaatg agtatgaaga attttcaaga
    17041 agacatgaaa aagaaaaaat aaattatgtg gacataaatt caataaaaag taattgttat
    17101 ttaggaattg atgcaggctc aactacaact aaagcagcac ttattgatga ggatggaaga
    17161 cttgtatatt cttattataa tagcaatgaa ggaaatccat taaaaacaac cattaaagtt
    17221 ataaatgaag tatatgatat attaccaaaa aatattaaaa tattgagttc aactgtaact
    17281 ggatatggag aaggtcttat taaaaaagct cttaaaatcg ataacggaga gatagagact
    17341 atagcacatt acaaagctgc taaatttttc aataaagatg tagattttat acttgatatt
    17401 ggtggtcaag atatgaagtg cctaaaaata aaagatggag taattgataa tataatatta
    17461 aatgaggctt gttcatcagg ttgtggctca tttttagaga catttgcaag ttctttatct
    17521 atgacaatag aagaatttgc acacgaaggt atttattctc aaaatcctgt agatttaggt
    17581 tcaagatgta ctgtatttat gaattctaga gtaaaacaag ctcaaaaaga aggtgcaagt
    17641 gttggagata tatcagcagg gctatcttat tcagtaataa aaaatgcttt atttaaagta
    17701 ataaagatta gagatttaaa tgagattggt aataatattg ttgtacaagg tggaaccttt
    17761 tataatgatt tggtgcttag aagctttgaa aaactaattg gaaaaaatgt aattagacct
    17821 aatatttcag gaattatggg agcttttggt tcagcactta tagctaaaga aaaatatgaa
    17881 agtggataca aaacatcctt gttatctaag gaagagttga ataatatcaa gctagaagca
    17941 agtgtaacta gatgtaaagg ttgttcaaat cattgtcttc ttacaataaa taaattttct
    18001 gataatgaaa tttttatttc aggtaataga tgtgagaagg gtgaagctat ttatggtgat
    18061 aaaaaaatag gattagaaaa gaaaaaacca ataaatttat ttaagtacaa atacaataga
    18121 atttttagat ataaaccatt ggaaaaagaa gaagcaaaaa atggggaaat agggatacca
    18181 agagtattaa atatgtatga agattatcca ttttggttta cttttttcaa tgctatgggc
    18241 tttagagttg tactttcaga taggtcatca aaacagttat atgaaactgg tataactagt
    18301 attgcatctg aaacagtttg ttatccagga aaattagttc atggacatat agaaaattta
    18361 attgcaaaag gtataaaaaa tatattttat ccaagtgtaa caaatgaaaa taaagaggat
    18421 tcaaatgcag ataattatta taattgtcct gttgtaatat catattctga agtaataaaa
    18481 aacaatgtag aaaatataag aaataagaac ataaattata taaatccatt tataagtcta
    18541 aatgataagg aaaaattgaa gaaaagacta tatgatgaat tatcaagaca tttttctgat
    18601 ataaaaataa ctaaagaaga agttaatcat gcagttgata aagctactga ggaacagaat
    18661 ttatttaaaa tggagataca agcagcaggt gaaagagcac ttaaagaaat aaaagaaaga
    18721 aatatgaagg ggattgtgtt atgtggaaga ccatatcata tagacccaga aataaatcat
    18781 ggaatgccag agcttataaa ttcattagat atggcagtgc ttactgaaga ctctatatgt
    18841 catttagcca atgtacaaag accacttaga gttgtagacc aatgggtata tcattcaaga
    18901 ttgtataaag cagctagttt tgtgagagat aaaaactatt tggaacttgt tcaacttaat
    18961 tcatttggct gtggattaga tgcagtaact acagaccagg ttcaagaaat acttaatgaa
    19021 aaatcgaaga tatatactat tataaagatt gacgaaggca acaatttagg agcagctaag
    19081 ataaggatga ggtccttaaa agcagctatg tttgagagag aaagcaaaaa tatagatgta
    19141 aaaaatataa aggtaaacag gatacagtat gctaaaaata atcagataac gaacaaacat
    19201 actatattag caccacaaat gtctccaata catttccaat tccttgaaaa agctgttaac
    19261 ttaagtggtt ataatataga aattctaaaa gatactgata gtagtgtaat tgaagaaggg
    19321 ttaagatatg taaataatga tgcatgttat ccagctataa tagttgtagg tcagctaata
    19381 acagcattaa aatctggcaa atatgattta aataacactt ctgttactat aactcagact
    19441 ggtggaggtt gtagagctac aaattatatt ggattcttaa gaaaggctat atatgatgct
    19501 ggatttaaag atgttcctgt cattgcactt agtgtaaatg gaatagaaga tagtggtata
    19561 atggataata tatcactaaa attaatcaat agactcttta tgtctgttgt ttatggtgat
    19621 ttgctgatga aagtactata tagagtaaga ccatatgaaa aagttactgg aagtgcaaat
    19681 gctctatatg aaaaatgggt tgatatatgc aaatcttctc ttgttaaagc aaaaatttct
    19741 gtatttacta gaaatataaa agatatagta aaagaatttg ataatttaga gatactagat
    19801 atcaaaaaac caaaagtagg tttggttgga gaaatattgg tgaaattcca tccaattgca
    19861 aataataatt tagtagatat attagagcga gaaggtgctg aagcagtcgt gccagacctt
    19921 acaaatttct tcttaagttg tgcctttaat actatataca aacacactca tttagaagga
    19981 agtagaaaga gtagaatgat aggagaggca tttatataca taactggaat atatcaaaga
    20041 gtatataaaa aagcacttga taagagtgag agattttatg ctccagccaa tataaaaaat
    20101 gttgctaaaa gtacagaacc agtagtgtca ttaggtaatc aaactggaga aggttggctt
    20161 ttaacaggtg aaatggttga attattaaat gaaggtgtag aaaacattat ttgtatgcaa
    20221 ccttttggat gtttacctaa tcacataatt ggtaagggtt ctataaaaga attaaagagg
    20281 ttgtataaaa atgcaaatat aatacctatt gattatgacc cttctgcaag tgaggtaaat
    20341 caacttaaca ggataaaact tatgctttct aaggctttta aaaatatata agatttatat
    20401 tttgatatgt taaaagttaa tgaatttaaa aatatactct atattaagta tctataaaaa
    20461 agagttatcc tattttttag ggtaactctt tattagttaa aaaattatat gtatactgat
    20521 attaaagggg ggaagtaata tgagtttagt atatgatgtt tataaatcaa aattaggaaa
    20581 tttgtacata ttaagtgaag atggttttat agttagtata tacataggag atgaaaaatt
    20641 taataattta aaaaatgaat gtattaaaaa aggaattgat ataaagagaa gtaaggaaga
    20701 tttaaaggaa gcaataagcc agatagatga ttactttaat ggaaaaagaa aagtctttaa
    20761 tttaaatatt cgtattgaag ggactgaatt tcaaaaatca gtttataggg aaatgttaaa
    20821 aataccttat ggagaaacat tgtgttattc agatatagca agaaatataa acaatccaaa
    20881 atcagtaaga gcaataggac aagcaagtaa aagtaataaa attccaataa taataccttg
    20941 tcatagagta gttggcaaaa aagatattgg tggatatatg gggaatcatt ctgatttgaa
    21001 agagatacta ttaaatttag aaagacagag ttaacttaga aagaaatgtt tttattgtta
    21061 atatttgata taataatagt aaaaagaagt attttttaca aaaataataa ggaatttatt
    21121 ttaatttaca aaaatgaggg gggattttaa tgtgtgataa agttatagca tcaaaatatc
    21181 atgagaaggg atttaattgt gcagaatcag ttataaaagc atataatgag gaatttaata
    21241 cagatatacc agtttgttta ggaagtggac tgggttcagg gtgtggggtt gctagtttat
    21301 gtggtgcagt aaatgcatca aatataataa ttggatatgt aaaaggcaga aaccacgaag
    21361 atgaaagtac taaagctaaa gtatatgcta aagatttaac tacaacagta agaaaagagt
    21421 atggttcaga gttatgtata gacctaaaaa aagatttagt tgcttgtaga gaaataatgg
    21481 atttcgcata tgatagtcta aaggaaactt taaaaaaaga attataagaa aataagctat
    21541 caaatataaa ttatttgata gcttattttt atagccgata ctaaataaat caaatatatt
    21601 ttagaattat tatttgtaaa taaacagcaa ataattttag gctttttatt taatatagtt
    21661 t
//
LOCUS       DAKBNN010000047        21204 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000047 DAKBNN010000000
VERSION     DAKBNN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.047"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            249..770
                     /locus_tag="OUB02_001547"
     CDS             249..770
                     /locus_tag="OUB02_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885310.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            804..1580
                     /locus_tag="OUB02_001548"
     CDS             804..1580
                     /locus_tag="OUB02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8885311.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1923..2876
                     /locus_tag="OUB02_001549"
     CDS             1923..2876
                     /locus_tag="OUB02_001549"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HCT8885312.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2894..3913
                     /locus_tag="OUB02_001550"
     CDS             2894..3913
                     /locus_tag="OUB02_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HCT8885313.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3944..4195
                     /locus_tag="OUB02_001551"
     CDS             3944..4195
                     /locus_tag="OUB02_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885314.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4477..6282
                     /gene="lepA"
                     /locus_tag="OUB02_001552"
     CDS             4477..6282
                     /gene="lepA"
                     /locus_tag="OUB02_001552"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCT8885315.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6431..7768
                     /locus_tag="OUB02_001553"
     CDS             6431..7768
                     /locus_tag="OUB02_001553"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCT8885316.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIHCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8001..9314
                     /locus_tag="OUB02_001554"
     CDS             8001..9314
                     /locus_tag="OUB02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8885317.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9486..10661
                     /locus_tag="OUB02_001555"
     CDS             9486..10661
                     /locus_tag="OUB02_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCT8885318.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10764..11783
                     /gene="hrcA"
                     /locus_tag="OUB02_001556"
     CDS             10764..11783
                     /gene="hrcA"
                     /locus_tag="OUB02_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCT8885319.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11813..12433
                     /gene="grpE"
                     /locus_tag="OUB02_001557"
     CDS             11813..12433
                     /gene="grpE"
                     /locus_tag="OUB02_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCT8885320.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12525..14372
                     /gene="dnaK"
                     /locus_tag="OUB02_001558"
     CDS             12525..14372
                     /gene="dnaK"
                     /locus_tag="OUB02_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCT8885321.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14583..15737
                     /gene="dnaJ"
                     /locus_tag="OUB02_001559"
     CDS             14583..15737
                     /gene="dnaJ"
                     /locus_tag="OUB02_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCT8885322.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16703..17638
                     /locus_tag="OUB02_001560"
     CDS             16703..17638
                     /locus_tag="OUB02_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT8885323.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17674..19047
                     /locus_tag="OUB02_001561"
     CDS             17674..19047
                     /locus_tag="OUB02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8885324.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLANAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19235..20002)
                     /locus_tag="OUB02_001562"
     CDS             complement(19235..20002)
                     /locus_tag="OUB02_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885325.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20032..21141)
                     /gene="ugpC"
                     /locus_tag="OUB02_001563"
     CDS             complement(20032..21141)
                     /gene="ugpC"
                     /locus_tag="OUB02_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCT8885326.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         8285 a         2397 c         3738 g         6784 t
ORIGIN      
        1 aaatttatat gcttaattaa gataattttt ccaataatca agataatttt tttcattagt
       61 atgtatgata gatgcatcaa acaatataac taatatgtta gaaggttata tgtaattaat
      121 ctcagtgttt atatagaata gcatttgtag catttattat agttgtaatt actacttata
      181 tcatagactt gaatagtcta aaaaaataga ctataatatt tttatagact aaagaaaggg
      241 ctgataagat ggacatagca aaactaattt taaatcctgc aaggcttcgt atcttacaat
      301 atattcgact tcatggcagt gttcgtacat ctgatattgt aaagtattta aatgatattc
      361 ctcgtgcaac agtttatcac catgtaaaaa ttttagaaga gaacaatatg attgaagtaa
      421 ttaaagaaaa tcgtgtgcgt ggtactattg aaaaggttta tgcactaaag gaatatacaa
      481 catctatgga aggagagacc ttcgttgcac tttctacagc attttatgta ggtctgatgc
      541 aggaaatgaa tgaatacttt agcagaaaag agcaagacca taaaaaggat aatgtatttt
      601 tctcttcagc attactatat atgagtgata atgaatatga aaatcttctt aaaagtatag
      661 ctgatttatt gaaaccatat atagaacaaa agcctaaatc aaatctaaaa cttagaaaac
      721 tttcaattat atcttctcca cctgtagctg ataatgataa aacaaaataa catgcaaccc
      781 atttttataa aaggaggtta tatatgaaat ttcatgagtt tggaaataaa aacagaccac
      841 atattatgtt gattcatggg ggagggaatg catggtggaa ctatttgcga caagcagaag
      901 ttttgtctaa caaatatcat gtgatacttc ctacattgga tggtcatgga gaagaatatg
      961 aaacaacata catttctaca gaggatacag cagataagtt gatatcatat atagatgaaa
     1021 attgcagtgg acatcttttc gctttgtgtg gggtatcact tggtggtcaa attgtcatgg
     1081 aacttttatc tcgaaaacct aatattacta aaaaagcaat tattgatggt agtatttgct
     1141 acccaagacc tcttatggca aggttttgta ttgctggtat tcgatttttt ggaggacttt
     1201 tgtttagtga gaaggcttgt cgctttcaaa ttgctatgat gccaaaattg ttacctgaaa
     1261 atatgcaata tccagatgaa ataaaagcat attatatgac agatatgcca catgtaagaa
     1321 aagaaacatt ctataatatg tatcgtactt atatgatgaa ctatacactt aaagagaatg
     1381 ttaaaaaaac cacagcacaa gtaatgtact ggtatggaga aaaagaaatg aagtgtgtca
     1441 aaaaatctgc aaaactgttt cagtcttatg tgccctcttg tgagatttat gaagctaaga
     1501 gctataatca tgggtattta tcactttatc ttccaaatga atggttggaa attgcagaac
     1561 catttttcca aaaggaataa aggtgttagg tgattattgt ataattattg ctgtttttta
     1621 atatagtaaa taaattaggt ataaatataa aaaagttatt ttagttaata tattagtgaa
     1681 agcttaatag gaaaattata aatagattaa aggtagcgcc tatataaata gttgctacct
     1741 ttttctgttt gtaaataaat gatagataag aaggtttact gacaaatcac tgactaaacc
     1801 caattttctt acattgaaag ccttaatttt acttgtttta aataaattta caatttcttg
     1861 ttataaaata ctaaattttg ttaacaatta taattagcat ttaatttata ctggaggttt
     1921 atatgattag tgtaagaaca gatttagctt tagaagctag tgaaatgtgt gaaaagtctc
     1981 aagaaggaag tagtattcct ggtgtaaaaa tagaaactaa agaattagaa aattgcattg
     2041 taactaaagt tgaagtaata gacaggcaag gcgcagagat aatgaataaa gatataggaa
     2101 agtatataac tctggaaagt aatcttatga agtttgatga tgatgagtca agagaagaaa
     2161 tgataagtta tttaaaagag gagttagtag atatatttgg ccaagacaaa aataaaaaaa
     2221 ccctggtaat tggtcttgga aatagaaata ttacttctga tgcgttagga ccaaagagtg
     2281 tatcaaaaac acttgtaact agacatcttt ttaaaaatta taataaagac tatgatgatg
     2341 atttcactga ggtatcagct ttaagcccag gagttatggg tgttactgga atagaaacta
     2401 gtgaaatagt aaaatcattg gttgagaagg ttaagccaga tagagtagta gcaatagatg
     2461 cattggcttc aagaaaaatg gagagagtaa actctactat acagatatca acagcaggaa
     2521 tatcacctgg tggaggtgtt ggcaatacta gaaaatctct aactaaagaa actttaggcg
     2581 tggatgttat tgcaataggc gttccaacgg tagttgatgc agcaactctt acaatagatg
     2641 tcttagatat ggcaatagac aatttaatag ctcaatcaga agaaacagaa agtttttatg
     2701 aaatgttgaa aaaacttaaa gaagaagaaa aatatcatct aataaaagat tcgttagacc
     2761 catatgacaa gaacttaata gtaacaccaa aggatataga tgatacaatc gaaaatctat
     2821 ctataataat aagtgaaggc ttaaataggt ctttacatcc aggtagattg gtataaaata
     2881 aggaggagca attatgttta aaaaatgcat taaagtagta accttgactt ttatattagt
     2941 atgcatttta ccaggaaaat cattagcttt gaatcaagat gattttttaa agtttttagt
     3001 aaattcatct tatccagaag ctaaagtcga aggaaatgat acagaaaata aaaagaataa
     3061 taaaaataaa gaaacaagta aggaaaataa ggaagaaagt aaagaagaaa acactaaatc
     3121 taaagatgca agtaaagtcg ataataaaaa agagagtgaa aaagaatata taaaacttta
     3181 tgttggtaaa gaaaatgttc cagatattga gtcaaaaaat tcagacacaa ctgaaactaa
     3241 cacaacatct agttcagatt ataaggatga tttaagagta accaaagaaa atccaagaat
     3301 tttgatatat catactcatg gatgtgagac ttattcaaat tcacctgatg gaaattatca
     3361 ttcaagagat aagaaaaatt cagtaatgga agtaggaagt gcattaacta gtgctttaga
     3421 tagcaaagga tggggtgtag tccatactac taaataccat gactatccat cttataataa
     3481 ctcttatgca agtagcttaa aaacaataca aagtatatta cctaaatata actcagtgga
     3541 tatagcgata gacttacata gagatgctag ggatttaact aatccagcca ctaaggaaaa
     3601 agaccattta aaatatacaa ctatgataaa tggagagaga gtatcaaagt tcttctttgt
     3661 tgtaggaggt aaaaatacaa atagaaaaca acttagagct ttagcagaag atataactgc
     3721 ttttgctgag aaaaagtatc ctggtctagt atctccaata gtagaaaaag actatgctag
     3781 attcaatcag tttgcagtta agaatcatat gttagtagaa atagggaata acgcaacaag
     3841 tgtagaagaa tctaaggcta ctacaaagta tttagccgag attttggatg aatattttaa
     3901 acaaaaaaat tagagcttta agttataatt aggagcatgc taaatgagcc gattagaaaa
     3961 aaatatagaa agtaaaagta aactaagaaa atacaagtat attatgagaa tcttattttt
     4021 gattactatg attatatgta catcagtttg tgtatttata gtggacagta atgcaaaaag
     4081 tatgctagga atgaaacaat atgattttcc aagtattaaa tttgatataa caaaatttga
     4141 ttttgacata ccaaattttg atatacatga aatcataaaa aaatttaata agtagtaata
     4201 agtgaataag gtttattgat taaaataagc cttattttag ttatatattt ttaatgtata
     4261 aaataaaaat tcattaactg tattttatag gatttacttt aagtttacga tattatataa
     4321 tatttagtgt tagacttgat aatccacata taatgtggat aattttgttt agtgccaatt
     4381 agtttatatt caaaaatttt aaatttggta tataaagagt ttttagataa tgtaaaataa
     4441 taactataag aataatcaag aggagggaat tttaaggtgg acaataaaca aagtagaact
     4501 agaaatttta gtataattgc acatatagac catggaaagt ctaccttagc tgatagatta
     4561 atacaacaaa ctgggcttgt tagtgaaaga gacatgaaaa gtcaattact tgacaatatg
     4621 gaccttgaaa gagaaagagg aatcactata aagcttcaaa acataagatt aatgtataaa
     4681 gcgaaggatg gaaatgaata ttatttaaat cttatagata cacctggaca tgtagacttt
     4741 aactatgagg tatcaagaag tttagctgca tgtgaaggag cactattagt agttgatgca
     4801 gctcaaggtg ttgaggcaca aactttagcc aatgtttatc ttgctataga ccaagattta
     4861 gaaatattac caataataaa taaaatagat ttaccaagtg caagaccaga ggaagtaaaa
     4921 aatgaaatag aagatttaat agggcttgat tcaagtgaag ctcctcttat ttcagctaaa
     4981 actggtttaa acatagaaga tgttttagaa gatattgtaa aaaatgttcc tccacctaaa
     5041 ggagataatg aagctccatt aaaggcatta atattcgatt cttactatga tgcttacaaa
     5101 ggtgttgtag cttatgttag agtatttgaa ggaactgtta aaaagggtat gacaataaaa
     5161 atgatgaata ccaataaaaa gtttgaggta actgaagttg gtgtcatggc tccaggtcaa
     5221 actgaactta gtgaattatc agcaggtgat gttggttata ttgctgctag tataaaagat
     5281 ataagaagtt gtcgtgttgg tgatacaata acagattcaa ataatcctac agaagaacct
     5341 ttaccaggat acaaaaaagc tactccaatg gtatattgtg gtatatatcc aggagaagga
     5401 gaaaagtatg agaatgttag agatgctctt gaaaagcttc aagtaaatga tgctgcactt
     5461 gaatatgaag ctgagacttc agcagcacta ggatttggtt ttagatgtgg tttcttaggt
     5521 cttttacata tggagataat gcaagaaaga cttgagagag aatttaacct tgatataatt
     5581 actacagctc catctgttat atatagagtt acaaaaatgg atggagaggt tgtaatgata
     5641 caaaatcctg caaacttacc agaaccatct gagataaaga tgatagaaga gcctatagta
     5701 aaaggagata taatagttcc taaagactat gtgggtgttg ttatggagct ttgccaggaa
     5761 agacgtggaa atatgttaaa tatggaatat atagatgaga gaagagtaat gcttcactat
     5821 gaccttcctc tcaatgaagt tgtttacgat ttctttgatg ctttaaaatc gagaactaga
     5881 ggttatggtt ctcttgatta tgaggttaaa ggttatgtag catctacact tgtaaaactt
     5941 gatatattaa tcaataaaga gcaagtggat gcacttagtt ttattgtcca tgagacacgt
     6001 gctttcccta gaggaaaggc aatgtgtgag aaattaaaag gagaaattcc aagacatcag
     6061 tttgctatac caatacaagc tgctgttggt aataaggtta ttgcaagaga aacaataagt
     6121 gctctaagaa aagatgttct tgctaaatgt tatggtggag atatatctcg taagaagaaa
     6181 cttcttgaaa aacaaaaaga aggtaagaag cgtatgagac aaattggttc tgttgaagtt
     6241 cctcaaaaag catttatgtc tgtgcttaaa ttagatgagt aaatccttat aaatttagat
     6301 aaagatattt aaaagctaga gtgattttag aattatttct aaattagtct agctttttta
     6361 tggtatattt taagtaaact catagagaat ataagtaaga atattaaaga agaataaaaa
     6421 ggtgatattt atgattaata tagaaattcc taaaaaagta gactatataa ttaaagaatt
     6481 agaaaagaat ggttatgaag cttatatagt tggaggatgt gttagagatt gtttattaga
     6541 aagagttcca aacgattggg atataacaac tagtgctaga ccagaagtgg tagtagaatt
     6601 atttgaaaaa acaattccaa ctggtataca acatgggact gtaacagtta tgattgaaca
     6661 tgaaccattt gaagtgacaa cttatagaat tgatggaaat tatagtgatg gtagacatcc
     6721 agattcaata gaatttacaa ataacatagt taaggacttg tcaagaagag attttactat
     6781 caattcaatt gcatacaatt caaagacagg attagtcgac ccatttaatg gctatgaaga
     6841 tatacaaaat aagtatatac attgtgttgg aaatccagta gatagatttg aagaagatgc
     6901 acttagaatg cttagagcag ttagattttc agcacaactc aattttaaaa tagctgaagg
     6961 tactaagcaa agtattcata aaaaagcaga tttgataaaa aatgtttcta ttgaaagaat
     7021 ccagacagag tttaataaaa tattagtatc tgattcatct aaattaaact tattaaaatc
     7081 aacaggtttg ttgaaattta taattcctga aatatgtgaa ttagaagatg ttactcaaca
     7141 caatccatat cacatctatg atgtacaaaa acatacttta atagctacag aagcaattga
     7201 agatgaactg tatttaaaac ttacaatgtt gtttcatgat ttaggtaaaa aagtaactaa
     7261 aacaactgat aaaaatggtg ttgaccattt ttatactcat agtagagaat ctgtaaagat
     7321 tgcaaagaag attttgaaaa gacttaagta tgataattat actattaata aagttttaat
     7381 attaatacaa tatcatgatt atagaataga acctaaaagg aagataatta aaaagttatt
     7441 aaataaactt gaagatgttg gattatttga agatttaata aaagttaatt gggctgatac
     7501 acttgcaaaa aatccaaagt atgcaaaaca aaagatatta aatcttattg agtgtgaaaa
     7561 agaatttaaa catataataa atcaaaagga gtgctttaat cttaaagatt tagctattaa
     7621 tggaaaagat ttgatatcta taggtataaa accaggtaaa gatataggat atattttaaa
     7681 taagatgctt gaaatagtca taaataatcc agagttaaat gaaaaagaaa tactaaaaga
     7741 aaaagcatta aatatatata cattttagat tcaaatataa aaaatttaat tactttaaaa
     7801 aacatagaat aaaatactta tttagataaa ataaataatt tttagagaat attttagaag
     7861 ttacgattgt atgattttta aatgcatagg catttactct aggactatag actaatataa
     7921 aaaaaaactt tgggaatact aaaaccataa atttataaaa ttaatatttt atatactgta
     7981 aaacagtgag gagggacagt atgcatactc aactacaaaa gactattggt ctttcagcag
     8041 ctctttctac tgttgtaggt atggttatag gttcgggagt tttttttaag ccacaagcaa
     8101 tatacactac aactaatgga gctccaggtc taggtattat agcgtggctt ttaggtggtt
     8161 ttataacaat aactgctggg cttacagcta cagaaatttc agctgctata ccaaaaactg
     8221 gtggtatgat gatttatatc gaagaaatat atggagaaaa gttaggtttt ttaactggat
     8281 ggatgcaaac tgtattattt ttcccaggta catcagcggc attaggagta atatttgctc
     8341 aacaagcatc tgaactttta ggaatgagcc caaataatat ggcaaatgta cttcctatag
     8401 caattggagt tatactattt ttagcgttat taaatataat aggttcatcc ttaggaggaa
     8461 aagttcagac agtagccact attggaaaga tgataccttt aattttaata atagtctttg
     8521 gatttattaa aggtcaaagt agtgaagtat taaatccatt tgtaggagat ggtgtaaatg
     8581 catcaaatgc acttggacaa gtattaatag ctactttgtt tgcatatgat ggttggataa
     8641 atgttggagc tatatcagga gaaatgaagt ctccagagaa agatttacca agagcaatag
     8701 ttggtggttt atcattagtt atggcagttt atattataat aaatatagct tatctttggg
     8761 tagtaccagc aagtgaatta gcaacagtta cttccccagc tacattagta gcaacaagat
     8821 tgtttggaaa tataggtgga aaagtaataa ctgtgggtat attaatatca gtatttggga
     8881 cattgaatgg atatttatta acaggttcaa ggatacctta tacattagct gaaatgggaa
     8941 ctttaccagc atctaaaact cttttaaaag taaattctgg aggttctcct gtaaattcaa
     9001 tactacttat aactgtgtta gcctgtgtat atgcattatc tggtcaattt aatcttttga
     9061 cagacttaac aatattttca atatgggtat tttatgtatt gacttttata ggtgttatga
     9121 ggcttagaag acaaaagcca gatttacaca gaccatataa agtcccttta tatccaataa
     9181 taccaattat agctatatta ggtggactgt ttgtaataat aaatcaaata ttgacttcaa
     9241 cagtcatatc tttaggcgga atatttataa cactattagg tttaccagtt tattattata
     9301 tgaaaaaaag ataactactt aatttaagtt agataaattt gggtaaaggt gaaaattaac
     9361 ttttatccaa attttttata ctgttataca atataagtat tatatattgc ttgatgtgct
     9421 attttgtttg atttataatt tagtaattga tgttttaaga taatttaaga aatgaggagt
     9481 gaaagatgct aggactttat gtacatattc cattttgtgt gaaaaaatgt aagtattgtg
     9541 attttaattc ttataaaatg gacatagatt caaaaaaaag atatatagaa gacttaaaaa
     9601 tagaaatgga attatatagt aataaattat ataaagataa taaatataaa aataaagaat
     9661 gctgtagtct aaataaaaat gacaaaataa cgagtatatt tgtaggggga ggtacaccta
     9721 gtatactaac ctctgatgaa ataagagaag tatttataag tataaaagaa atgtttgata
     9781 tagatgagaa tgcagaaata actatagagt gtaatcctgg gacattaaca ttagaaaaat
     9841 taaaaactat gaaagagatt ggcataaata gacttagtat agggctacaa gctattcaag
     9901 aaaagcattt gaactttata ggaagaatac atacttatga agaatttgag aaaaactata
     9961 aagatgcatt aagtgtaggt tttaaaaata ttaatgtaga tttaatgtac agtcttccaa
    10021 atcaaactct atgtgattgg aaggaaactc ttgaaaaagt tgtacactta aatccaacac
    10081 atatatcagc ttattccctt atactagaag aaggaactga actatacaat atgtatgaaa
    10141 gtaataaatt tgaacttata gatgaaaatg tagatataga aatgtatgag tatacaataa
    10201 actacttaaa gtcaaaagga tataatcaat atgaaatatc aaactactca aaagaaggct
    10261 ataattgtga gcataatatt ttatattggg aatgtgaaca ttatataggg attggagcag
    10321 gagcttctgg ctatattaat gagaacagat acaataatgt agaatcttta gaagactatc
    10381 atttaagttt ggtaaaaaga gaaaaaccaa tacaagaaaa tgaaattctt tctgagaaag
    10441 atatgataga agaaaagata tttatggggc ttagaatgaa taaaggcata aaatttgaag
    10501 attttaaaaa aaaatttgga atagatttta gagagaagta taataagcaa atagaaatgc
    10561 tattagctag aaagcttata aatcagagct ttgaaggtat acaattaact caaaaaggaa
    10621 gagaaatatc taatagtgta tttatagaat ttatggaata atttttaagg tgttgacaaa
    10681 ataaatttat aatggtatat aatatatata atctttttag cactcgaact tagtgagtgc
    10741 taacagctag gaggtataat aatatggaat taaatgaaag aaaattaaat atcctcaaag
    10801 ctatagttaa agattatata gaaacagctg aagctatagg ttctagaaca atatctaaaa
    10861 gacatgattt aggtgttagt gctgctacca taagaaatga aatggctgac cttgaagaat
    10921 tagggtattt aattcagcct catacttctg cgggtagagt tccatctgaa aaaggctata
    10981 agttatatgt aaactctctt atgagtaaaa gcgaattgga tgataatgat aagattctta
    11041 ttgaacaatg tatgaatcac aatataaatc atataaaaga attgattcat gaaacttcta
    11101 agctattatc gcaactaaca aactatacaa ctgtagcagt tactaagagt ttaataaatc
    11161 aaagtgttat aaagcacata cagttggttg ctatgaatga caataatatt ttacttatag
    11221 ttgtaacaga taaaggggac ttaaaaaaag ctaaccttac aactaatgta tatttagatc
    11281 aatctaagtt aaatttgatt tctgataatc ttacaaggaa gctcttaggt aaaagtataa
    11341 cagatttgga tgataatcta attgctttta ttaaatacga aataagtgag tattctgggc
    11401 ttatagatga gcttttaaat gctttgaact caaatatgaa agaagaagat ttttctctat
    11461 cattaaatgg agctactaac atatttagtt atccagaatt taatgatgta ttaaaagcaa
    11521 agtcattttt aaatatgttg gaaaaaaagg aaacaatagc tgacataatt aaatcaaagg
    11581 gaatacaaaa agacaatcta aatataataa ttggtagtga taatgattgt gaactggcac
    11641 aggattgtag tatagttaca gcaacttata atgtagatag agacttggtt gggcgaataa
    11701 gctttatagg ccctaccaga atggattatg ctagaattta ctctataata aattatatga
    11761 gtttattaat taatagaaag taaagaaatt ttaaattgcg aggtgtaaag tcttggataa
    11821 aaaaaatgag caacaggaag taagagaaga aaatgatact tctattaacc aagaatcgga
    11881 aactcaagta gagttagaag aggaagttgt taatgaagaa tgtgaaacat caagtgaaaa
    11941 aactgatgaa aaagaagttg atgatgaaaa tgtaacagat ataaattcta agttagcaga
    12001 aaaaaaattg caggatgagt tggatgaatt aaatgataaa tatcaaagac ttcaagccga
    12061 atatgcaaac tacagaagaa gaactcaaca agagaaagag acaataggcg tatttgcaaa
    12121 tgaaaaaata ataactgaat tgataccagt tatagatagt atggaaagag ccttagatgc
    12181 atgtgaagat aaggaagata ctatgtataa gggtataagc cttgtacata aacaattaat
    12241 agacacttta gtaaaatttg gagtagaaga aatagaagca gaatctaagg aatttgaccc
    12301 taatctacat ctagcagtta tgcaagaaag tgtagatgga gttgaagcaa atcaaatagt
    12361 aatggtactt caaaaaggat ataagctagg aactaaagtt gtaagacctt caatggttaa
    12421 agtttcttgc taagtataaa ttaaaggtta aattatttaa cctaatataa aatattaaaa
    12481 taagtaaaac ttaattatat aattactcga ggaggatttt agtcatggga aaaataatag
    12541 gaatagattt aggaacaaca aattcatgtg ttgcagtgtt agaaggtgga gaagcacaaa
    12601 taatagcaaa tagtgaaggt atgagaacta ctccatcagt agtagcattt acaaaagatg
    12661 gtgaaagaat agttggagaa cctgcaaaaa gacaagcagt tacaaatgca gataaaacaa
    12721 taacttctat aaaaactcat atgggaactg attataaagt aaatatagat ggaaaatcat
    12781 atactccaca agaaatatca gcaataatat tacaaaaatt aaaatcagat gcagaaagtt
    12841 acttaggaca aacagtaaca gaagcagtta taacagtacc agcttacttt acagatgctc
    12901 aaagacaagc aactaaagat gctggtagaa tagcagggct tgatgttaag agaataataa
    12961 atgagccaac agcagcagct cttgcttatg gtatggataa attagaccaa gaaaagaaaa
    13021 tattagtatt tgacttaggt ggaggaactt ttgacgtatc tatacttgaa ataggagatg
    13081 gaacttttga agttttagct acagctggta acaatagatt aggtggagat gactttgacc
    13141 aaatagtaat agattattta gcagaggaat ttaaaaaagc tgaaggtgta gatttaagaa
    13201 atgataaaat ggctcttcaa agattaaaag aagcagcaga aaaggctaag aaagaattat
    13261 catcaacaat gagttcaaac ataaacttac ctttcataac tgctacagca gaaggaccaa
    13321 aacacttaaa tatagattta tcaagagcta aatttgaaga attaactaga ggtttagttg
    13381 aaaaaactat ggaaccaact aagacagctt tacaagatgc aggactttct acaggtgata
    13441 tagatgatgt attattagta ggtggttcta caagaatacc agcagtacaa gaagctgtta
    13501 agaagtttat aggaaaagaa cctcataaag gtataaaccc agatgagtgt gttgcagcag
    13561 gtgcttctat acaagctgga gttttagcag gggatgttaa agacttatta ttactagatg
    13621 ttactccatt atctcttggt atagaaacta tgggtaatgt aatgactaag ataatagaaa
    13681 gaaatactac aataccaact aagaagtctc aaatattctc aactgctgct gataaccaaa
    13741 cagctgttga tatacatgtt cttcaaggtg aaagaagtat ggcttatgac aatacaactt
    13801 taggaagatt ccaattaaca gatataccac cagcacaaag aggaatacct caaatagaag
    13861 ttacttttga tatagatgca aatggtatag ttaatgtttc agctaaagat ttaggtacag
    13921 gaaaagagca aaaaataact ataacatcaa acactaattt atcagaagca gaaatagagc
    13981 aaaagataaa agaagcagaa atgaatgcag aagctgacaa acaaaagaaa gaaaaaatag
    14041 aagcattcaa tcaagctgaa tctactatat atcaaactga aaaaacttta aatgaacttg
    14101 gagataaaat aagttcaggt gaaaaagagg atatagaaaa agctatagca gatttaaaag
    14161 ctgtaaaaga taatcaagat gctacagcag aagaacttaa gaaagctaca gatgaagtaa
    14221 tgactaagtt ccaaaaagta tctcaagaaa tgtaccaaaa agcagctcaa gaacaacaag
    14281 cggcacaagg tgcagaacaa gcacaagaca atggacctaa agatgacaat gtagtagatg
    14341 ctgactttaa agaagtagat gaagataaat aagctatata atatgtaaca ctaaatgata
    14401 attggaacat atatgatttg tgaaaatcat atagatgtta aagatatatg gacaatatac
    14461 atcataaaga atataatata tcatagatta tataacttaa agtagaatat aagaattaat
    14521 gatagcttgt agtttattat tctacaagct atcgttttgt aaaatgaagg tggtgtcaat
    14581 ttttgagtac aaaaagggat tattatgaag tcttaggtat cagtaaaggt gcagaagctc
    14641 aagagataaa aaaagcttat agaaaattag caatgaagta ccatccagat agaaatcctg
    14701 gagataaaga ggcggaagaa aaatttaaag agataaatga agcttatgaa gttctatctg
    14761 atgatacaaa gagaaaaact tatgaccagt ttggacatga tgggctaaat ggtcaaggcg
    14821 gttttggtgg tcaaggagga tttggcggtc aaggctttgg tggttttgaa gatatgttcg
    14881 gagatatatt tggtgacatg tttggaggaa gcttcggagg tggaagagca agaagaagag
    14941 gacctcaacg tggtgcagat ataagacaga gtgttactat atcttttgaa gaagctgcat
    15001 ttggtaaaaa gatgtctata aaagtaaata gaagtgaaga gtgtgaagaa tgtaatggaa
    15061 ctggagcaaa accaggaaca tcaaagaaaa cttgttcaac ttgtaatgga actggtcaag
    15121 ttagaactgt tcaaagaact ccatttggta atatagcaag ttcaagacca tgtagtgctt
    15181 gtaatggaac tggagaagtg attgaatctc catgtagtaa atgtcatgga actggaaata
    15241 caagaaaagt taagactata gaagtagata tacctgctgg tatagatgat ggtcaaatga
    15301 taaaactttc tggtcaaggt gaagtaggag aaaaaggtgc tccaagagga gacctatata
    15361 ttgtagttaa tgttaagtca catccactat ttactagaga tggaaatgat atttattttg
    15421 aaatgccaat tacatttgtt caagcaactt taggtgatga gatagaagta cctactttgg
    15481 atggaaaagt taaatatagt gtgccagagg ggacacaaac tggaactgta tttagactta
    15541 aagagaaagg aatccctaga attagaggta attcaagagg agaccaatat gttaaggttg
    15601 tagttgaaat tcctaaaaaa ttaaacgata agcaaaaaga actattgaga gaatttgcga
    15661 aggagtgtgg gtcgaacgtc cacgaaaaga agaaaacctt cggacaaaag atagaagata
    15721 tgtttaagaa aaagtagtta aagtagccaa tatataatca tatttttaaa atcttagtta
    15781 tttataagat tgtcaaagga gatattatga tttttatatt ggctattttt gtataaaaaa
    15841 taaatgtgca taaaataaaa ctgaatataa ataattttct gaaaaatact tgtcctaaaa
    15901 tatataatgt gctataatat aatcaaataa aagaaagggg tgattccagt gggaacggat
    15961 gtggcactgc caaatgatga agaaaacttt attgtaatat acaaaataaa attagatact
    16021 tttagtgcta gattaagtac tggaaaaact catgagctgg gataattatt gtaattacct
    16081 agactcaaac ttttagtagt aaaatagtaa tattatatag acaaggtatc taatttttaa
    16141 atcttttgta tgaattaaaa tttttataaa gggtatatct gattagaatg taattaggga
    16201 aacctagttg tgaattactt ttagtatata catgtgtaaa gaaatatgta tatggtattg
    16261 gttattgtgc tagatgtgta tgtaaagaaa tatgtgtcta gttgggtata tgtaaagaaa
    16321 tatacttata atctatgaaa aatttttagt ataaattgct aaaacattga gattgttttt
    16381 tatagaaagt tagtttatac caactaaaaa tgtgcttaag atttgtttga tgtttaatta
    16441 taaataaggt tgtagataaa aaaataagta aagacaaagg tttaaaatac ttgctttaaa
    16501 tatagtttgt tttgaacact tgccaaatta aaataattaa taaaatagac atcttctcat
    16561 agtaaaatat actgtgagag gatgtttttg ctttgttcat aaacttttat gaaaaatttg
    16621 catagtgtca cattttaata tactatatat ataataaaat atagataaaa ttaaatgatt
    16681 caaaagagaa aggtgaaaag agatgtatta tataggtgta gacataggag gaacaggtat
    16741 acaagcagga gtagttgata actatgggaa aataattttt aggtcagagt gtaaaacagt
    16801 aattgaaaaa ggatttgaag gaatacttaa tgacataaaa ataatgattt ataagttgtt
    16861 ggaagataat aaacttacta tgagtgacat aaaatctatt ggatttggtg ttccaggttt
    16921 tataaataaa gaaggattag taacttgtgt aaatttaaaa tggaataaaa aagcttttaa
    16981 taaagagtta aaaagaagat ttccagatgt tgaaattcat ggagaaaatg atgcaacagt
    17041 agcagcactt ggagaagcaa agtttggaag tatgaaaggt gctaatgttg gagtattata
    17101 tactttagga actggtgtag gtggaggaat agtaattaat caaaaagtct tttctggagc
    17161 acatggattg ggttcagaaa taggtcatca aattatagga gaaaactatt ttaattgtaa
    17221 ttgtggaaat aatggttgtg tagaaacttt ttgttcagca actgcaatta taaagtattc
    17281 tcaaaaactg atagaagaag gagaaaagag taggatttta gatttggcag agggaaattt
    17341 agaaaatgta aatgcaaaga tggtgtttga tgcatataga gaaaatgatt tagtggcaat
    17401 taaagtaata aatagattta aagagtattt agcaaaaact tttgcaaaca caataaattc
    17461 tttagaccca gaaattatat ctataggagg aggcatttct aaatctagcg atataatact
    17521 tgatggaata gaagatttgg taagaaaatt tgtattatac aaaacagaag atatagctac
    17581 aattacatgt gcaactttag gttcagacgc aggaattata ggagcagctt ttttataatg
    17641 taaatgaact gcttattgga ggcgtgatga tatatgggta aaacaaatgc tagaagaacg
    17701 ctttttttga tagcaatagg ttcaggaact atgctgaatc ctcttaattc ttctatgatt
    17761 tcacttgctt tacatagtat acaaaatgat tttcatatta gttttacaac tgtatcttgg
    17821 attgtttcag cattttacct agctaatgct attgcacaac cagtttcagg taagatagga
    17881 gatttaatag gtagaaaggt cttatttttg agtggtttga tgcttgtaat tttatcagca
    17941 tccatagtac cacttgttca atcattcttt atattgattt ttataagagt aattcaagca
    18001 attggaacaa gtaccctata tccatctgga gttgctctta tacaaaataa tataaaagaa
    18061 agacaatctt ctgcattagc agttctaacc atttttgctt caactacagc aggattaggt
    18121 ccgacacttg gaggattatt gttggactta ggaggatggc atgctatatt tcttgtaaat
    18181 atacctgttg ttttggtttc tatatgccta gggtattttt tattcccaaa agaagtaaaa
    18241 gaaaagaaat ctataaaaga aactttaaaa aatgttttgt caagattaga tattattggc
    18301 atacttctat tttcaattgc aatgattttt atattactat ttttgctatc tatgaaggaa
    18361 tcttttaact tagaacaact tgtatttggt ataatactga tgtgtctttt tatttggcat
    18421 gaattgagaa caaaattgcc atttatagat attaagcttt ttgtaagtaa tcctaaattg
    18481 tcaaaagttt acttgcaatt tattatatta aatttcttta attatatttt gttttttggt
    18541 cttcctagtt attttcaaga tgccttacat tatagtgcaa agtcgactgg attatttatg
    18601 ctatttatgt ctggtatagg tatatttata tctccattaa ctggaaaatg gatagataaa
    18661 tctggaacta gatttcctct tgttacatca tcaatcttta tgtttattag tgcagtactt
    18721 ctttcagtag tttttatcca tatacctgta ataggaaagg gtattatatt atcattagca
    18781 ggtattagtt atggagttgg taatgtagcc ctgcaatcct ctatgtttga agaaagtcct
    18841 cgtgacagta tagggacagc ttctggtctg tttcaaacta gtcgttattt aggttctatt
    18901 ttagcaactg ttttacttgg aatggtattt gaagtaaata taacatcaga acaatttcag
    18961 atattaggtt atgttatggt ggtcttaggt acaataagtt ttttattaaa ttttatatta
    19021 aagaaagaat taagaaaagt agaatagtgg atttatggat ttaataagtc taataactga
    19081 taagtttaaa aatattgttt gtaaaacata actggtttga tatttattaa ttctaagttt
    19141 tgaagcatag agtactcgat aagtatacaa tattttgaat taaaattaag cagaaatttg
    19201 aactagtctt tcaaatctct gcttaattta gtggttaaat ttttttatag agaaaataag
    19261 atttgacact tagataaaaa atcattgcat tttcaaaatt ctttaaatca aacccaagta
    19321 tctcattaat cttttccaat ctatatacta gcgtgtttct atgtaaaaac atactagtag
    19381 atgtcttaga aatatttaaa tcattattta taaattttat tcctgtatat agaagttctt
    19441 catcaataga tttaatctta ttaaaatcta ttaatttatt gagtatatta tcctcattaa
    19501 ttaaatctaa agttttatat ctaataaggt caaacaaatt taaaaactta ttttcagcgt
    19561 tgtcttttat aatatgaggt agtaaatcag aacataactg taatctaaaa tcaaaatcag
    19621 ctttactatc taatttacca agataaaaaa tataagtact ttgataagtt tcagtttcaa
    19681 ttatattttg tgtagttaca ttaatatcta agttatattt agaatttact ataaaaaaac
    19741 cattgtgttg tattatattt atatcttcaa ataaattata aaaaatattt tttagatatt
    19801 caaagtctat atgagattgg taaattaaat aaaagttgcc ttttaaatta tcaagattat
    19861 ttttatctag aaaatcaata acataaatag cattattgaa agtaactaaa tggctactag
    19921 catctaactt ttggttttca tatggataga tatttatttt tttattaagc tgtctttcta
    19981 aaaaatcttt taactcttcc ataaagtctc cttaaaaata agattatatt cttatcttat
    20041 agctaaagta gtttctttgt caaatatgtg tactctacta ttgtcaactg aaagtgttaa
    20101 gtcatcacca acattaattc tgtttttact gtcaaatcta attgttatcg tattttctcc
    20161 tagttttaag tgagcataag tttctgctcc cataagttct tttatttcaa ttcttgattt
    20221 aaaacaagta tctggattag atttaataaa agtatcttct acatgaatat cttctggtct
    20281 gatacctata acaacttcat tattaatata attgtctttt attaaatcat agtgtttttc
    20341 tttattcaat ttaagcttaa tatgttcatt ttgtgcataa atttcatcat tttcttctat
    20401 taatattaca tcaataaaat tcatctgtgg tgctccaata aatcctgcaa caaatatatt
    20461 agctggataa tcgtatacat cttgtggagt tgcaatctgc tgaactactc catccttcat
    20521 tactacgatt ctatcagcca tagtcatagc ttcaacttgg tcgtgtgtta catagataaa
    20581 tgtagttcca agctgttggt gtagcttagt tatctctgtt ctcatagcag ttctaagttt
    20641 tgcatctaag ttggatagag gttcatccat taagaatacc ttaggatttc taactattgc
    20701 acgtcctaag gcaacccttt gtctttgtcc tccagataat gttttaggtt ttttatttaa
    20761 aagaggtagt aagtctaata ttttagcagc ttctttaact tttttgtcta tttcatcttt
    20821 aggcaatttt ctaagtttaa gtgcaaatgc catgttttcg taaacagata agtggggata
    20881 taaagcatag ttttgaaaaa ccatagctat atctctatct tttggttcta tatcattgac
    20941 aagtttatct ccaatgtata gttctccttc agagatttct tcaagaccag caatcattct
    21001 aagtgtagtc gatttaccac aaccagatgg tcctactaaa acgataaatt ctttatcttt
    21061 aatgtctata tttatatttt ttactgcatt aaatccattt gagtaaagtt ttgatatatt
    21121 ttttaaaatt acttctgaca taatatcctc cttgatattt aaattttaat tatatagtat
    21181 caaatttttt tattatatta aatg
//
LOCUS       DAKBNN010000048        21118 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000048 DAKBNN010000000
VERSION     DAKBNN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.048"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            168..1247
                     /gene="mltG"
                     /locus_tag="OUB02_001564"
     CDS             168..1247
                     /gene="mltG"
                     /locus_tag="OUB02_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCT8885327.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            1419..2093
                     /locus_tag="OUB02_001565"
     CDS             1419..2093
                     /locus_tag="OUB02_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCT8885328.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            2080..3327
                     /locus_tag="OUB02_001566"
     CDS             2080..3327
                     /locus_tag="OUB02_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCT8885329.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            3399..5063
                     /locus_tag="OUB02_001567"
     CDS             3399..5063
                     /locus_tag="OUB02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCT8885330.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQILIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(5160..5801)
                     /gene="sigK"
                     /locus_tag="OUB02_001568"
     CDS             complement(5160..5801)
                     /gene="sigK"
                     /locus_tag="OUB02_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HCT8885331.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            6368..6730
                     /locus_tag="OUB02_001569"
     CDS             6368..6730
                     /locus_tag="OUB02_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885332.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            6714..7430
                     /locus_tag="OUB02_001570"
     CDS             6714..7430
                     /locus_tag="OUB02_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885333.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            7450..7869
                     /locus_tag="OUB02_001571"
     CDS             7450..7869
                     /locus_tag="OUB02_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885334.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            7859..8383
                     /locus_tag="OUB02_001572"
     CDS             7859..8383
                     /locus_tag="OUB02_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885335.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIIGNSMGIMNISNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            8695..9252
                     /gene="efp"
                     /locus_tag="OUB02_001573"
     CDS             8695..9252
                     /gene="efp"
                     /locus_tag="OUB02_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCT8885336.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            9344..9799
                     /locus_tag="OUB02_001574"
     CDS             9344..9799
                     /locus_tag="OUB02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8885337.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            9962..10672
                     /locus_tag="OUB02_001575"
     CDS             9962..10672
                     /locus_tag="OUB02_001575"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCT8885338.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            10726..11796
                     /locus_tag="OUB02_001576"
     CDS             10726..11796
                     /locus_tag="OUB02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8885339.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     IFQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            11824..15378
                     /gene="smc"
                     /locus_tag="OUB02_001577"
     CDS             11824..15378
                     /gene="smc"
                     /locus_tag="OUB02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCT8885340.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRNVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            15405..16685
                     /gene="ftsY"
                     /locus_tag="OUB02_001578"
     CDS             15405..16685
                     /gene="ftsY"
                     /locus_tag="OUB02_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCT8885341.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     FEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEIV
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            16788..17156
                     /locus_tag="OUB02_001579"
     CDS             16788..17156
                     /locus_tag="OUB02_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCT8885342.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNKD
                     CANLIPKLENIEDVCREMIK"
     gene            17153..18505
                     /gene="ffh"
                     /locus_tag="OUB02_001580"
     CDS             17153..18505
                     /gene="ffh"
                     /locus_tag="OUB02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCT8885343.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            18543..18815
                     /gene="rpsP"
                     /locus_tag="OUB02_001581"
     CDS             18543..18815
                     /gene="rpsP"
                     /locus_tag="OUB02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCT8885344.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            18880..19107
                     /locus_tag="OUB02_001582"
     CDS             18880..19107
                     /locus_tag="OUB02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCT8885345.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            19215..19730
                     /gene="rimM"
                     /locus_tag="OUB02_001583"
     CDS             19215..19730
                     /gene="rimM"
                     /locus_tag="OUB02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HCT8885346.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            19739..20434
                     /gene="trmD"
                     /locus_tag="OUB02_001584"
     CDS             19739..20434
                     /gene="trmD"
                     /locus_tag="OUB02_001584"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCT8885347.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            20619..20969
                     /gene="rplS"
                     /locus_tag="OUB02_001585"
     CDS             20619..20969
                     /gene="rplS"
                     /locus_tag="OUB02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCT8885348.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
BASE COUNT         9015 a         1993 c         3866 g         6244 t
ORIGIN      
        1 aatgttaggt tttttatttt ttgataaact ataaattata ccaaaaatat atattttaaa
       61 acatttttta caatataaat aagtttattc tacaagatga ttgaaaatta aagtatctag
      121 ttttataata gaaaatggat aaatatacat aaggagtaga ttatattatg aactttaaag
      181 aaaatagatt gaaaatagca gtattaataa ttgttatttt gattatctta gcaggtatat
      241 ttgtgtttat acaaataggt ccttatgata aaaataacaa aaaagatgta attatcgacg
      301 ttccaagtgg agcatctgtt ggaaaaatat ctgacatatt atatgaaaat aaattaataa
      361 agaatgaact attatttaaa cttttggtaa aggttagcaa taaagctccg tctataaaat
      421 caggaacata cttacttaat caatcgtatt caaacaatga tataataagt cttttagttt
      481 caggtaaaat atatcaggat ggtataaaag tgactattcc agagggagct acatctaaag
      541 aaattatagc aatgttagta agtaaaaatt taggagataa agctactttt gaaaatttaa
      601 taaaaaaacc tcaagaattt tatgataaat ttccatattt gaaggaagat ggaataacct
      661 ctttagaagg gttcttgtat ccagaaactt attattttaa ttcaaagaaa caatcagaag
      721 aagatatttt aagcgaaatg ttaaaagtat ttgacagtaa atacacggac aagtttaaaa
      781 agaaacaaaa agaattaaat atgactcttc aagaagtaat ggaaatggca tcaataattg
      841 agaaagaagc tgttcttgac aaagacagac ctataatagc aagtgtgttt tataatagac
      901 ttaaggtagg tatgcctctt caatcagatg ctacaataca atatatattt gaagagagaa
      961 agaaaattgt aacatatgat gatttaaaaa ttgattcacc atataatagt tataaaaata
     1021 agggattacc tcctacacca atatcaaatc ctggtataaa atctattgaa gcagcattat
     1081 atccagagaa aacagactat ttatattttg tagctaagat tgatggtgga aataattata
     1141 gtacaaacta tcaagaccat ttaaaatacg ttaaagaata taaagaagca agggataaac
     1201 aatcaaaaga tacaaaagca acaaataaag aaaatacaaa aaaataaacc atgattataa
     1261 aaagtctaac agggtaagaa taatgttggg cttttttatt gaaaattgtt attcttagat
     1321 ttataataaa gaaagtgtaa atatatataa caatatgaat ttaaaaaata ataaattaat
     1381 ataaaacttg aatatttatt attttaggga gtgtttttat gagtaatata gttaatgatt
     1441 tggttgaaga ttatataaga gtgacattaa aagaaaaaga aggattttta aaagatttag
     1501 aaatttatgc agaggaaaat agtgttccta taatacataa agaagtttca gatttattaa
     1561 aagtcttgtt aaaggttcaa aaaccaaaga gaattttaga agtaggatgt gcaattggtt
     1621 attcttctat tttttttgca actattatag gaaaagatgc agatataata actgttgaga
     1681 gaaatgaaaa aatgatagaa aaagcaaaag aaaacataaa attagctgga tttgacaata
     1741 acataactat actagaagga gatgcagaag aaaaacttag tcaagtccaa ggtgaatttg
     1801 accttatatt tatagatgca gccaaaggtc aatataagct attttttgat ttagttatag
     1861 ataaattaaa agatggggga cttttagtat ctgataatat tttgtataaa ggtatggttg
     1921 ctcatgatga ttttgttgta agaagaaaaa agactatagt taaaagaatg agaaattatt
     1981 tagattatat atgtaattgt gactatttaa gcacatcatt ggtaccaata ggtgatggtg
     2041 tagcactaag ttataaagaa agtaaaagag gtgatgtaga tggaattaaa taaagtagaa
     2101 cttttagctc cagcaggtga tttggagaga ttgaaaatag ctataacata tggagcagat
     2161 gccgtttata taggtggaga aatttttgga atgaggtctg cagcgaagaa ttttagtaag
     2221 gaagatatgg cagagggagt agcttttgca catgaaagag gcaagaaagt attcgtaaca
     2281 gtaaatataa tacctcataa tgaagatttt ctacaattag aagactattt gttggaacta
     2341 gaagagatag gaatagatgc agttataata gctgacccag gagtgcttag tgtcattaaa
     2401 aaagtaattc cgaatatgga aatacattta agtacacaag caaatacaac caattatcta
     2461 agtgcaaact tctggtacga gcatggaata aaaagagttg tagtggcaag agaattatct
     2521 tttgatgaaa tatctgagat aagagcaaaa acacctttag atatggatat agaagcgttt
     2581 atgcatggtg ctatgtgtat ttcttattct ggcagatgtt taataagcaa ctatatgact
     2641 ggaagagatg caaacaaagg ttcttgtgct caatcttgta gatggcaata tcatttggtg
     2701 gaagaaaaaa gacctggtga gtattttcca atatacgaag atgaaagggg tacattcttc
     2761 tttaactcaa aagatttatg tatgatagag tatataccag agttaatcaa atctggtata
     2821 acaagtttaa aaatagaagg tagaatgaag actgcatact atgtagctac tgttgtaaga
     2881 gcatacagaa tggcaataga tgaattttac agagacccag aaaactggaa gttcaatcca
     2941 atgtggatgg aagaactaaa aaaaggtagt catagacatt ttacatcagg tttttacttg
     3001 aataaaccaa caacagaaga ccaaaattat caatcagcat catatgttag aaactatgat
     3061 tttataggta tagttagaga aacagaagat gaagatggtc ttattgttgt tgagcaaaga
     3121 aataaaatgt gtgttggtga tgaaatagaa gttatgggac catataaaga aactatgttt
     3181 actaaaattg aagaaatgta caatgaagaa ggagaagcta tagaatcagc tcctcatcct
     3241 agacaaatag tgaagttaaa actatcagta aaagttggta aagattatat gcttagaaaa
     3301 gttatagaag aaaaggttga agaataataa aaaattgata aaaatagtat tagcccctta
     3361 gaaattgtca ataatgaaat tatctaaggg gtgatttcat gtcaaaaaag aagactccat
     3421 ttttgaaaaa agtgggaaag agaagctggt gtatatttac tataatttta ataatttata
     3481 gtgttttaat ttataggcta gttgatatac aagtattaaa aggtgataaa tacaaacaaa
     3541 gtgttgaatc acaaagcgtt gagaaagtcg agttaaatag tggaagagga ataatctatg
     3601 atagaaacaa caagaagctt acagatacaa gtaaatctca gatattaatt gttgaaaaag
     3661 aaaaattaaa taataactat aaaattttgg aacttattaa gaaagctact aagatgaatg
     3721 atttagatat atataaagct gtacaagagc aattgacacg ccctataata cagattcaga
     3781 ctaaaaatat cgataagagt atgaaaaaag aacttgagaa aaatggaata atggtagaag
     3841 aaaaaactat gaggtacgca aaagatggtt tattatcaca tacaatagga tatataaaag
     3901 aagatgataa atcaggtcaa tcaggaattg aaaagagtat ggatagtgta cttagaaatt
     3961 caaatgaaaa gtatataagt gcttttaaag ctggagatgc aggaaatgaa aaatctctaa
     4021 acatactaaa gggaagtgta aaaactgttg ataataagga taaagataga catctaaaaa
     4081 ctactattga ttataacatt caaaaaaaat tagaacaaat actaaataaa gaagaaaatc
     4141 ctacagcagc aataatctcg gaagcatcta caggtgaaat tcttgcaatg tgctctagac
     4201 ctaattttga ccaaaatgac atatcaaaaa gtttaaaagg aaaaaatgga gaatttgaaa
     4261 atagagttat aaaagcgaca tatccaccag ggtctgtatt taagatggta gttttatttt
     4321 ctgcacttga aaatggtgtt atagatgaga actatactta caactgtaca ggtaagacta
     4381 aagttgggaa tactaatgaa atactgcgat gtaataaaag agatggtcat ggttttcaaa
     4441 acttaagaca agctttttct aattcatgca atcctgcatt tttagatata gctatgaaac
     4501 ttggaaaaga aaagatactc aaatctgctg aaaaattaca tttatttgaa aaggttgata
     4561 ttggtcttga tgaagaaaaa attagagaag ctccaaaaaa catatcaata agaaatttag
     4621 ctataggtca agaaaatata gaatttacac cacttcaaat aaatcaaatg actcaaataa
     4681 tagctaataa tggaacattc aaaccactat acttatataa aagtcttgtg gacaataata
     4741 tgaatacaat aaaaacttat aagtcttcaa aaaaagaaga acttatttct ccttatgtgt
     4801 gtacacaagt taaagaatat atgaagtctg tatctaggac tggtacagct aaagacctaa
     4861 aagatattga aggtggttgt ggagttaaaa ctggtacagc tcaaagtagt ttaaataaaa
     4921 aagctataga ccacggatgg ataacaggat tttatcctga agaaagacct aagtatgtaa
     4981 taactgtgtt agtagaagga actcaaaaag gaaataaatc tgcaacacct atatttaagg
     5041 aaatatgtga aagtataaaa taaattatgt actataaaat ttaaggatat atcgtaactt
     5101 tgattttaga tagaaaattt tgtctaatga tgtacgatat atccttttaa aatatagtat
     5161 taactttctt gaacaagctc tttttctagt tttttaagag cttttttttc gattctagat
     5221 acatatgacc ttgatatatc gagtttttgt gcaatttctc tttgtgtctt atagccataa
     5281 ggagttaacc catatcttaa ctggacaatt tctctttctc taggagtaag aattttgttt
     5341 agttgttcgt aaagttttcc aacttgaact tttagttcaa cttcatcaag aacatagtta
     5401 gcctctgtac caagtatatc taaaagactt atttcattac cttctttatc tgttccgata
     5461 gggtcttgta gagatacttg agttttattt tttttgttag atctaatatt cattaaaatc
     5521 tcattttcaa tacatctaga agcatatgtt gctaatcgag ttcctttatc tatgttataa
     5581 gtttctatag ctttaattaa tcctattgta cctatggata ttaaatcatc ctgatcttcc
     5641 gttgaattat tatatttctt tgctatataa gctactaatc tcatgtttct ttcaattaat
     5701 gtatcttttg cacttttatc gttttctatt ttaaatttag ttaaatactc aatctcttct
     5761 tcaggggtta agggtttttc aaaagattta agagctgcca ataggtcacc cccttttcaa
     5821 cggtcacttt aaattatatg aaggagactt atccaagttg actgttatct aataaaataa
     5881 tatgcatatt tttagaattt aaaacatata tttgcaaact tttgaatgga atatgacata
     5941 aagagtggga atttaaattt aagtttttgt tgtatatata tgaaacaagg cgaagaaatt
     6001 attgatttat aaagtataaa ttggaattga ggtgtcaata aatataaaag gaaatagtat
     6061 ttatatatgt tttggtgttg aagtaaaaga ttaatgcaaa gtattagagt ctataaaaat
     6121 atgaatatag gaggtgtaat tttacaatat ttaaaaaagt aaatgtaatt aattgtttgt
     6181 ataaagttga ttacaagcaa aagtatcagt catagttaat actgttaagg atgaaggtgg
     6241 tgtgttaatt tatatatgta tgggaagaaa ttttgattac aatgaaaaaa cttacaaaag
     6301 atatttacaa aaaaacttat agttaaatgt ttatatataa aatactattt aggggttgtt
     6361 tttattattg aaaataagaa agagtggttt tatatcaatc gaatgtataa taagtattgc
     6421 tatattatat gtggctgttt atttagtttc tacatcattg tataattgtt atagttttat
     6481 cagtagaaat atatctgaca gagaaatgtt aagtacagca aaaaaatata tagaagatga
     6541 gaagtataga atacaaaata gtaagtatga gttaattgaa gataagatag aaaaaaatta
     6601 cataaatgga tatgaaatta acagtagaat agagcaaatt ttagattatt atcaatgcta
     6661 tgaaataaat atagagataa aaaatgaatt taaaaaactg aggtttaata gctatgttac
     6721 tagaaaataa aggctctata ttaataataa cattgataat attttctatt ataagtactg
     6781 tgtgcattat gtgtataggt ttaatttata cacataataa tatgtttgag ttaggataca
     6841 aagacattca actaaaagaa atttctttaa gtggaataga aatatcagta agtaatgtac
     6901 tttcttgtgt tgaggaagct attgatgtat ctaataatga agacgaattt agagaatatt
     6961 tcttaggaaa taatatgatt gaatgtataa aaaaaataaa agatacatct tatagtagtt
     7021 tagaagaact ttctttaaaa gtagaaaata atactattta tgacagagga gatttttttg
     7081 aatttgatat agaaagtaat gttagagaaa aagaattttt aaaagcaata aaagtaagag
     7141 tgaaaatcaa aaatccatgg ataggaattg acattgaaga ttatatggat aaatctaata
     7201 acaataaaat tgatgacaaa acattcaata attataaagc atctgaaaat aatgaaattc
     7261 taaatcaatt taaaaatgaa agtgaaaata taaggaatca agaggttggt ggatttacag
     7321 aagaagatga agaatatgaa gaagaactta aacaaaacat aattgaaaat ataaagaaga
     7381 attttgatga aaaaaaccta gtacaggtat ataagtatag gggtgtataa ctttgaaaaa
     7441 agaaggaatg tgactgtaat agaattaata gtatctatca gtataatact cttgatagtt
     7501 acattatctt ttccaaaaga taatcttgaa aatcataaaa taaatttatt tgcacgtcaa
     7561 ctttgttcag atataaggta tgttaggcga attaatatgt ttagtaacta caatacatat
     7621 atatattata taaatcaagg taaacacgaa ggttatgtat tgagaaaaga tggtaaaaac
     7681 attaaatcaa taatgctacc taaaaatgca aaaatagcta ggagtattga aataataaag
     7741 tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag agatatataa taatagatta
     7801 agaaaaacta taacaataac acctgtaagt ggaagagtac ttctgaaaga gggaaaatat
     7861 gaaacataaa tatggttact tgcttttgga aagtgtagta tcattgtctt caatggtgat
     7921 aattatacta gttttatatt ctatattttt gtctacaata aacttaaagt taaaagtaga
     7981 agataagata gaactacaac aacaaagtct tgaaatcata aaatctatgg aaggaattat
     8041 tggcaattct atgggaataa tgaatatatc taattatgaa gaaacattta aaaagacaac
     8101 gtctataaaa tgtagatatg tagatgaaaa taataatgaa gaaggcataa gtaataaaga
     8161 aattatattg aatgaaagac gaaataagtt atttgtaaat tctttaaatg gcgaaagtag
     8221 tcaagcaggt ggttatgaaa ttggagatta tgtagatgaa atgtatgtat caattactaa
     8281 taatggtcaa tatgttaata ttaaattgaa attatcaaaa cgtagtcaaa aatatgagac
     8341 tgatttcaaa ataaaagtat ggaattttag tgaaagtata taaaaataca taaatttaag
     8401 agaaagaaat gacatcaata agtcatataa aatataatta ttgagaacta tataactaaa
     8461 taatatcaat atgtaaagtg tatagattaa gaatatagag gataataata aaaatataga
     8521 ataagtaatt tgccaaatac ttgtttatat tgtataactt atgatattat gatattagta
     8581 aaaatttgta atcctgactt gacagaattc aagtaagaga tataatttta aaggcatagt
     8641 aatgtaaaat ttactaaaaa ataagaaatt tattttaata tggaggaaca aaaaatggta
     8701 tcagcaggtg attttagaaa aggtgttaca tttgaaaaag atggacaacc atgtttagta
     8761 gttgattttc aacacgttaa gccaggtaaa ggagctgctt tcgttagaac taaatacaaa
     8821 aacttaaaaa caggagctat aagagaagaa agtttcaatc caagtgaaaa attccctaag
     8881 gctgttatag atacaagaca aatgcaatat ctatacaatg atggtgagtt atattatttt
     8941 atggatcaag aaaattttga acaaatacca ttaaactatg agcaagttga agatgcaatt
     9001 aagttcttaa aagaaaatga agttgctaca ataagatttt accaaggaca accattccaa
     9061 gtggaagcgc caaactttgc agagctagaa gttacagata cagagccagg tataaaaggt
     9121 gatacagcaa gtaatgtaac taaagcagct acagttgaaa ctggagcagt tgttcaagtt
     9181 ccattattta taaatactgg agataaagta aagatagata ctagaacagg tgaatattta
     9241 tcaagagtat aaaaatttat gtatataaaa gcttatatgt cttaataata taagatatat
     9301 gagcttttag tttatatata aaaaagaaag cgaggggtgt tacatgaaac atttatatga
     9361 agaagttgca tacttaaaag gcctagcaga aggactagaa ataagtgctg aaagtaaaga
     9421 agggaaaatg atacataaaa tagttgatgc attagaagta tttgcagatg ctattgtaac
     9481 attggatgaa gaacaagaag aacttcaaga ttttgttgaa tctatagatg aagatttagc
     9541 agatttagaa gaagatattg ctgacatgga agaagatctt tatgaagaag atgacgatga
     9601 ggatgatgag gacttcagct atatagaaat ggaatgccca aattgtggag agttagtaga
     9661 aatagacgaa gatttgttat atgatgatga agtagatgtt gtttgtcctg attgtaaggc
     9721 tgtgatatta tcatcagaag atgattgtga tgatgattgt acatgtggtg gttgttcaag
     9781 ttgtgatgat agagagtaaa aattcataag aattaaaact gcctatgatg cctatgagat
     9841 attttgcttc ataggtagtt ttatttttat gaggaagggt attatgtatt ttggttttgc
     9901 aatacttgga caaaaatagt acactagaat ttatagaata ggattttgga gggatgtaaa
     9961 tatgaaaata agtaaaaaat tactagataa tatacaaaga tttgaaaatg ttataaatta
    10021 taaatttaaa aataaagaat atatactaga agctcttact catagttcat attctaatga
    10081 aaataaaaaa tataatttta acgaaagatt ggaattttta ggggattcgg ttttaggcat
    10141 agtgataagt gattatttat ttaatgaaga agctaatctg cccgaagggg aactaactaa
    10201 acttagagca aatatagtct gtgaagattc attaagtgaa gtagctaatg atataaattt
    10261 aggtattcac atgttattag gtagaggaga agaagcaaca ggaggaagac ataggacttc
    10321 aatattagca gacgcatttg aagctgtaat agctgctata tatcttgatg gaggttttga
    10381 aagtgcaaga cagtttatac ttcatcatat ggaaaatata atttatgatt caagaaaagg
    10441 aaatatattt agagactaca aaacgcatct tcaagaggtg ttacaaggga atggagaaaa
    10501 taatatctgg tacaggctta tagaagaaaa aggaccagac cataataaaa gatttgtaat
    10561 ggaagttgga attaatgacg atgtacttgg tataggagaa ggcaagagta aaaaagaagc
    10621 tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta tgggaaaaat aaatataata
    10681 tgtaaaaata taaaaagaaa tatttatatt agaaggatgt tgtagatgaa aaagagaatt
    10741 atacctatat ttgttcctca caaaggatgt cctcatgatt gcatattttg taatcaaaaa
    10801 aaaataacag gtgtgagtac tgatgttact agtgaagatg caagaaatat aattgaggaa
    10861 tgtttagaga ctatagataa agatgcagat gtagaaatag cattttttgg tggtagcttt
    10921 actgctatag atattgatat acaaaaaagt ttattatcag ttgctaaaga atatgtagaa
    10981 aaaggtcttg taaaagatat tagaatgtct acaagaccag attgtattga tgaagatata
    11041 ttaagtatgt taaaagaata taagacaagt ataattgaac taggagtaca atccttagat
    11101 gaaaaagtat tattagatag tataagaggt catcaatctg aaattgtata caagagttca
    11161 aagatgataa aaaatagtgg aatcaaactt ggattacaga tgatgattgg tcttccagct
    11221 gacacagaag aaaagtgtat atttacagca aaaaaatttg tagagctaaa accagattgt
    11281 gttagagtct atccaacttt ggtagtaaaa gatacaggtc ttgaaaagtt aatggaacaa
    11341 aataaatata atccatttac tcttgaagag agtgtaaata tagtaaaaaa agttcttgta
    11401 ctgttttatg ttaataatat caatgtaata agagttgggc ttcaagctac tgatgatata
    11461 caaattggaa aagctgtttt agcaggacca tatcatcctg catttagaga gttggtagaa
    11521 gcagatatga taaaagatta tttagaattt gttatttttc aaaataaaaa tataaagcaa
    11581 atgttggtca aatctaataa aaagaatatt tctaaaatca taggaaataa aaaaacaaat
    11641 gttaaatata tggaagaaaa atttggtgtg cttttaaaaa cacaagaatc agatttagat
    11701 ataaatcaat tagaaattgt gttagatggg aaaagtttaa ttagtgctaa tatgagagac
    11761 attcatagaa aattgtatga catttatgac ctttagtttt aataatgaag ggagagaaaa
    11821 gatttgtatt taaaaagact agagttaaag ggatttaaat cctttccagt aaaaacagat
    11881 attattttta aagaaggaat aacagccata gtaggaccaa atgggagtgg aaaaagtaat
    11941 atatctgatg ctgtaagatg ggtattagga gaacagagta ttaagagtct tagaggggat
    12001 aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa aacctatgaa ttattgtgaa
    12061 gtggctctta ctattgataa ttctgaaaat caattggaat tagattttac agaagtaact
    12121 ataagaagaa gggcttatag aaatggtgaa agtgaatttt ttctaaataa taaaagttgt
    12181 agattaaaag atataaaaga agtcttttta gatactggta ttggaaaaga tggttattca
    12241 atcatagaac aaggaaaagt agatgaaata ttaagtaata atcctttaag cagaagaaaa
    12301 gtttttgatg aagcttgtgg catatcaaaa tatagatata aaaaacagga agcagaaagg
    12361 aatttaagta atacaaagga aaatttagaa agaatagatg atgtatatat agaaattgaa
    12421 aatcaattaa agcctctttt taatcaacag acaaaggcaa aaaagtactt ggaaataagt
    12481 gaaaaattaa agacacttga agtaaatagc tttataagag aaattgaagg aatagaaaaa
    12541 gaattaagtg aggttaatga acatcgtaat gttattgaaa aggagctcaa tgaaaaagaa
    12601 gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta ataaagaggt agaggtatta
    12661 caagatgtaa ttgaaaaatc tgtagattat ataaattcaa ttaaaggagt tatatcaaaa
    12721 aaagaatccc aaataaattt aattaaggaa agaataagaa attttacgaa tgaaattagt
    12781 agaaaaaatt tagaaataaa ggatataaaa gagaagctaa atgaaaataa gcaatacata
    12841 aaagaattag aaagcaataa attatcaggt agtgaagaac tatctacctt gcaggaaaat
    12901 ataaaagtat tggaaggaag taaagataaa caaaaaatta agttagaatc cctaaataat
    12961 gagatagaat tattgaaaga aagtataatt gatatattaa ataagaaaca agagtttagt
    13021 aataagctgt ctactttaaa cgcaaataaa gaaaatatga atatcagaga tgaaaatata
    13081 aattcagaga taacagagtt aaacaaaaat attgaaataa aatcatctga attagatact
    13141 ataaataaag aatttaatat gcaaaatgaa aatttaaaga atgtaaataa tagacataaa
    13201 gaattatcaa ttaatttgca ggattcaatt agtgaacaca ataaactaga agatgaaata
    13261 cagaaaagta aatataattt aaatggatat aattcaaagt taaatgtcta tatagatatg
    13321 gaaaatcatt atgaaggatt taatagagga gtaaaagaag tactaaaaaa taaaaattta
    13381 aaaggagttc atggagcatt aggacagatt ataaatgtac ctgaaaagta tgagaaatct
    13441 atagaagcag ctcttggtgc ttatatgcaa aatataataa ctgataatga gtttagtgct
    13501 aagtctgcaa taaactatct aaaacagaat aatttaggta gagtaacatt tcttccatta
    13561 aatataatta aatcaaataa aataagtcta ggaaatttaa aagcaaatac taagttcata
    13621 ggaatagcaa gtgatttaat tacatttgat gaaaagtata gaaatataat tgaaaatata
    13681 cttggaagaa caatacttat aaataatata gatgagggca taaaatttgc aaaagaaact
    13741 ggtcataggt ttaaaatagt aactttagat ggtgaaatct taaatccagg aggttctttg
    13801 actgggggaa gtctaaaaac taatgggaat atactatcga gaaaaagata cattaatgaa
    13861 tatacagaaa agataagtaa tatcaagaat gaaatttcac atctagaatt aaaaagagaa
    13921 tctttagaca aagatgtgaa aaatatcaaa aatgaaattg attcacatga aagtaaaata
    13981 aaagatttag aaaaaagtat aataataaaa agtacaagta taaagaatgt tgagtctgaa
    14041 attgaatcgt taaaaggtag tattactaaa ttagaaaatg agaagaatga tttaaattct
    14101 aacctaaatt atacacttga aaaaagtgat gatgttagaa aagatatgga agagttagat
    14161 gatttataca ataaaaataa agaaaaaatt gatgcattga atgaggaaat aaaaagatac
    14221 aatgatttat atgataaaga aaaatctgaa tttgatgaat tgaacttaag tttagtgaag
    14281 aaaacagaag tttacaatag tatagttaga gatataaaaa gaatatctgg tgaaaattgt
    14341 gagctagaag aaaaaaataa acaattagaa gagtcattaa attatgaaga acatgaaata
    14401 attaaattac aagattctat actcactgaa gaaaaagaaa aagaaaattt aacaaaacaa
    14461 ctaggggata gtaatagaaa tcttgaaact agaaaaatag caaaagatga tttaaaaaat
    14521 agttttgatg aaattaataa agaattaaaa actatagata gacagcatat agaacttaaa
    14581 gaaagtttat ttaaagttgg tggaagatta gaaagattaa aaactagtca agacacatat
    14641 ataaataagt tgtttgagca atatgatatg accttagtac aagcattgga aattaaagat
    14701 gaagatttgg atatagatag aaaattctta gaaagtctaa agagagaaat aagaagtctt
    14761 ggaaatataa atatagactc tataaaggaa tatgaggaaa taaaagaaag atacgatttt
    14821 tatagcgaac aaaagcaaga tttagaagag tctatggaag agatagaaaa acttatacat
    14881 actttagaag aaaatatgaa atctgaattt gaaattaagt ttgaagaaat aagtaaaaat
    14941 tttaaatatg tatataagag attatttggt ggaggttgtg gagaattaac tattttagat
    15001 aaagaaaatt tattagaaag tgatatacta ataacagctc aacccccagg taaaaaaatg
    15061 aaaaatttaa acctattatc tggtggagag aaagctttaa cggcaataag tatattattt
    15121 gctatattga ttacaaagcc aacaccattt tgtatattag atgagattga agcgccactt
    15181 gatgacgcaa atatatttag atttggagaa tttttgaagg atttatctaa agagactcaa
    15241 tttatatcag taactcatag gagaggaact atggaagctg ctgattatat ctacggagtt
    15301 acaatgcaag aaaaagccat ttcaaaagtt attagtttaa aattaaaaga agctcaggaa
    15361 ataacagata ttatataact tagattgaca taggaggaat aacagtgtta aaaaaattat
    15421 ttggttttgg aaaagacaag gaaaaggaaa tagaaaaaaa agatgctgaa gaagaaatag
    15481 aagttgaaga cagtgttgat aatttagaaa acttggaaga aactattttt tcaggatttg
    15541 aagaagaagt gatagataaa gtggaagatg ttgaagaaaa aaatgaggaa agtgtcaata
    15601 atgaaggcac tgaagaaatc gaaaactttg aaaaaataga agttgagatg gatagtaatg
    15661 gaaaagaggt agaaaatatt tcaaataatg attctcaaga agagatagtc gaagaattag
    15721 aaaattatga tgaaattgag tctaaagaga tagaagataa agaagataag aaagttaact
    15781 tgtttgaaag actaaaacaa ggattgacaa aagcaaagca aggaataact gatagaatag
    15841 atgaagtttt aaaatcatat acaaagatag atgaagaatt acttgaagat ttagaggaaa
    15901 ttttaataac agcagatgtt ggtgtaaata caactatgga tataattgaa agactaagag
    15961 ataaaataaa gcaaaaaggt attactgaac caataaaggt aagagaagaa cttaaatcta
    16021 tagttgaaga tattcttaca aatgaaaact ctacactaga tatagaacca gcaccatgta
    16081 taatattaat ggtgggtgta aatggggtcg gtaagactac tacaataggt aaactagcaa
    16141 atagatacaa aaaagatggt aaaaaagttt tattagcagc agcagataca tttagagcag
    16201 cagcaacaga gcaattagaa atttgggcaa atagaaccaa tgtagacata ataaaacatc
    16261 aagagggagc agaccctgga gctgtagtat ttgatgctat aaaagctgca aaagctagaa
    16321 aaacagatgt attaatatgt gatacagcag gaagattaca caataaggct aatcttatga
    16381 atgaacttgg aaaagtattt aaaatagtag atagagaatt tccagaagca aaacgtgaag
    16441 tacttcttgt ggtggatgct acaacaggtc aaaatgcagt tgttcaagca aagacattta
    16501 aagaagttgc agatataaca ggtatagtac ttacaaaact tgatggaact gcaaaaggtg
    16561 gagtagtcct tgcagtaaaa tcagaagtag atgtacctgt aaaacttatt ggagttggag
    16621 aaagtgtaga agacctacaa gatttcaatg caaaatcatt ttcagatgca ttatttggta
    16681 actaatgttc ttttaaaaat aatggttgac aagagtgttg ttatgatata aaatacaata
    16741 gtgtaaagta aataaccttt acagtatact gctaggaagt gtaagatatg aatatagaaa
    16801 aaatggtcga aattggattg ttatttgaac agtataaaga attattaact gacaagcaga
    16861 aagaaatagt tgctctatac tatgaagaag attattcttt aggtgaaatt agtgaaaatc
    16921 tgaatgtatc aagacaaggt gtttatgata ccttaaagcg ttcagaaaaa atactaagag
    16981 attatgaaga aaagttacac ttagtatcaa agattcaaga gcaggaaaaa aatattaaga
    17041 ttataaaaga taaaattatt gacattaaag aagatttatt gcacaataaa gattgtgcta
    17101 atttaatccc taagctagaa aatatagaag atgtatgtag ggagatgata aaatgatatt
    17161 tgaaggattg tctgataaac tacaaggggc atttagtaag ttaaaatcta aaggaaaact
    17221 tacagaagta gatgtaaaaa atgctatgag agaagttaaa cttgctcttc ttgaggcaga
    17281 tgttaacttt aaagtagtta aagatttcat aaaaaaagtt caagagagat gtgttgggca
    17341 agaagttatg caaagcttaa ctccagctca gcatgtaatc aaaattgtaa atgaagagct
    17401 tacaagttta atgggagatg tacaaagtaa agtaatgatt tcctcaaagc ctcctacaat
    17461 tttaatgatg gttggattgc aaggtgcagg taagactact acagcaggaa aacttggagg
    17521 atattttaaa aagcaaggta aaaaacctct attagtagct tgtgatattt atagaccggc
    17581 agctataaag caacttcaag ttgttggaga aaaattggat atacctgtat tcaatatggg
    17641 agataaagaa aatccagtaa atattgcgaa agcaggttta agtcatgcga ttaaaaatgc
    17701 taatgattta gttattatag atacagctgg tagacttcat atagatgaag tacttatgga
    17761 tgagttaaag tctattaaat cagaagttaa gccacatgag atacttttgg ttgtagattc
    17821 tatgacaggt caagatgctg taaatgttgc agaaagcttc aatgaggcac ttggtgttga
    17881 tggagtagta ttaactaaat tggatggaga tactagaggt ggtgctgcac tttcaataag
    17941 agcagttaca caaaagccta taaaatttat ggggatggga gaaaagctag atgatataga
    18001 acctttccac ccagatagaa tggcatcaag aatattaggt atgggagatg tcttaagttt
    18061 aatagaaaaa gcacaagaaa atatagactt agagaaagct aaagaattag agcaaaaaat
    18121 taaaaaacaa gagcttgatt ttgaagattt cttacaacaa atggaacaaa tacaaaatat
    18181 gggacctctt gacaagatac ttggtatgat acctggtatg ggaaatgtaa aagaccaact
    18241 tggagatgta gatttgaatg gcaaagaaat gaagagaaca aaagccataa ttcagtcaat
    18301 gactacatat gagagaagaa acccaggagt attaaatgct tcaagaaaga aaagaatagc
    18361 taaaggcagt ggaactagtg tacaagatgt aaatagactt ataaaacaac ttaatgaaat
    18421 gaaaaaaatg atgaagatgt ttgctggttc acaaaagagt atgaagaaaa ggggcggctt
    18481 aggcggttta ccttttttta aataaaatat aaaaaagtaa aaattattcg gaggtgactg
    18541 taatggcagt taaaataaga ttaaaaagaa tgggagctaa caaaaaacct ttctacagaa
    18601 tagtagtagc tgattcaaga gctccaagag atggaaaatt tatagaagaa ataggatact
    18661 ataatccaat ttctgaacct aaacaagtaa gaataaatga tgaaaaagct ataaaatggt
    18721 tagctactgg tgctcagcca acagaagtag ttaaaaaatt acttgtaaaa aacggagtaa
    18781 tagaaaaatt cgaagcttct aaacaagcaa aataattttt tcttggcaat tagtttaagt
    18841 aagggctcat tgctaaggtt actttaggag atgaaatata tgaaagagct agttgtagat
    18901 atagctaagg ctctagttga taatcctgac tcagttgttg ttgaggaatt tgaagataat
    18961 gatggtatcg tcttaaagct tacagttgct caagaggata tgggtaaggt tattgggaag
    19021 cagggaagaa tagctaaagc tataagaact gtcgtaagat ctgttgcaaa tagagaaaat
    19081 ataaaagttt ctcttgagat agtataaaat aggaggatta ggtataatgc ctagtccttt
    19141 ttatataaat gcgtagttta agattttatg atgatatgac cacataagat taaatttttt
    19201 aagaaggtga taacatgaaa gaaaaattaa ctcattttaa ggtaggtcaa atagtaaata
    19261 cacaaggttt aaaaggtgaa gtaagagttt atccgcttac tgatgatata gagaggtttg
    19321 atgaattaaa agatttctat ctgggaaaag atttaaataa taaattggct gttgaaaaag
    19381 taagatacaa aggttctatg gttataatga agataaaagg catagacagc atagagaaag
    19441 cagaaaaact aaaaaataaa tttatgtatg tagctagaga agatagcaga gagcttgaag
    19501 aaggcgaatt ttttatagca gatatgattg gaatggaagt tttaactgta gatggaaaat
    19561 atgtagggat actagaagat gttcttcaat actcagcaaa tgatgtctat gtagtaaaag
    19621 gtgaggaaga taaagaattt atgataccag ctataaaaaa atttgtacct acaatagata
    19681 ttgatgaaag aaaaatgatt atagacccta ttaagggaat gatagattag gtggtaatat
    19741 gagatttcat ataatgacac tttttccaga aatatttaat tcgtacatgg atgaaagtat
    19801 aatgaagaga gcagttgaaa aaggtataat tgaggttcat atatataata taagagattt
    19861 ttctaataat aaacataaaa aagtagatga ttatccattt ggaggaggag cggggatggt
    19921 aatgactcct caacctatat atgacacata taagcatata ataacaactc ataatataaa
    19981 taacccaagt gtaatttatc ttactccaaa aggtaaggta tacaatcaat ctatggcaaa
    20041 gcagatgtct ttaaaagaag atataatact actatgtggt cattatgaag gaatagatga
    20101 gagaatcatt gatttaatag ttactgatga aatttcaatt ggagattatg tattaactgg
    20161 gggagagctc ccagcactta taatgataga ttctatatcg agattaatac caggtgtatt
    20221 aaaccaagaa gaatcatttg aagaagaatc atttaaagat aacttattgg aatatcctca
    20281 ttacactaga cctagagatt ttgaaggatt aaaagtacca gaagtgcttt tatcagggaa
    20341 tcataagaag atagatgagt ggagaagaga agaatctata aggataacca aagaaagacg
    20401 atttgattta tataaaaaat ctaatgaaaa ataaaaatct aatgaaaaat taatatatat
    20461 gtatacaata aagtacctat aaatggaatt aaaaacataa aagtgcttgt aataactatt
    20521 gaagtatagt ataatataaa atgttgaaaa tacgggcgtt cctctgttta cactggtcgg
    20581 gtagttaaga acgtctatga tatctaggag gaagaaagat gaatgaaatg ctaagagcaa
    20641 ttgagcaaga acaattaaaa aatgaagttc ctaattttgg acctggggac acagttaaag
    20701 tacacgttag aattatagaa ggaaaaagag aaagaataca agtattcgaa ggtgtagtat
    20761 taaagagaca aggtggagga gcaagagaaa ctttcacagt tagaaaaatg tctttcaatg
    20821 ttggtgtaga aagaactttc ccagttcact ctccaaaaat agaaaaaata gaagttacta
    20881 gaaaaggtaa agtaagaaga gctaaactaa actacttaag aggtagagta ggtaaagctg
    20941 ctaagataaa agaagctaga aataaataat taaaaacttt acttataaat ggggggctga
    21001 tttatcagtc ctctttctat tttaatgaaa attacagcaa ataattatac tagaaataaa
    21061 tagaaatatt tgttgaaaat atacattatt tatctatgaa tttatagctt ttaaaatg
//
LOCUS       DAKBNN010000049        20963 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000049 DAKBNN010000000
VERSION     DAKBNN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.049"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            534..1565
                     /locus_tag="OUB02_001586"
     CDS             534..1565
                     /locus_tag="OUB02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCT8885349.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1698..2996
                     /locus_tag="OUB02_001587"
     CDS             1698..2996
                     /locus_tag="OUB02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCT8885350.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3110..3355)
                     /locus_tag="OUB02_001588"
     CDS             complement(3110..3355)
                     /locus_tag="OUB02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HCT8885351.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3503..4369
                     /locus_tag="OUB02_001589"
     CDS             3503..4369
                     /locus_tag="OUB02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCT8885352.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGREPTTIICL"
     gene            complement(4373..4909)
                     /locus_tag="OUB02_001590"
     CDS             complement(4373..4909)
                     /locus_tag="OUB02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HCT8885353.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5100..5303)
                     /locus_tag="OUB02_001591"
     CDS             complement(5100..5303)
                     /locus_tag="OUB02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HCT8885354.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5594..6358
                     /locus_tag="OUB02_001592"
     CDS             5594..6358
                     /locus_tag="OUB02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8885355.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6747..8060
                     /gene="prdC"
                     /locus_tag="OUB02_001593"
     CDS             6747..8060
                     /gene="prdC"
                     /locus_tag="OUB02_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HCT8885356.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8464..8549
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9131..11278
                     /locus_tag="OUB02_001594"
     CDS             9131..11278
                     /locus_tag="OUB02_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HCT8885357.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDSQTPDVEKPSDGQMPDVEKPLDEQTPEEEKPLEEK
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSKDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGGEENELMTYSATQSAIWIILGQMDEQERLKQYQGSINLCDKLIERAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPP
                     VPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPSVSDTVEKTPELSRDDTIIK
                     SPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            11685..13445
                     /gene="prdR"
                     /locus_tag="OUB02_001595"
     CDS             11685..13445
                     /gene="prdR"
                     /locus_tag="OUB02_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HCT8885358.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13874..15754
                     /gene="prdA"
                     /locus_tag="OUB02_001596"
     CDS             13874..15754
                     /gene="prdA"
                     /locus_tag="OUB02_001596"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HCT8885359.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15835..16119
                     /locus_tag="OUB02_001597"
     CDS             15835..16119
                     /locus_tag="OUB02_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885360.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16157..16882
                     /gene="prdB"
                     /locus_tag="OUB02_001598"
     CDS             16157..16882
                     /gene="prdB"
                     /locus_tag="OUB02_001598"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16607..16609,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HCT8885361.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            16962..17720
                     /gene="prdD"
                     /locus_tag="OUB02_001599"
     CDS             16962..17720
                     /gene="prdD"
                     /locus_tag="OUB02_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HCT8885362.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17735..18202
                     /locus_tag="OUB02_001600"
     CDS             17735..18202
                     /locus_tag="OUB02_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HCT8885363.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18220..18702
                     /locus_tag="OUB02_001601"
     CDS             18220..18702
                     /locus_tag="OUB02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HCT8885364.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18722..19729
                     /locus_tag="OUB02_001602"
     CDS             18722..19729
                     /locus_tag="OUB02_001602"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HCT8885365.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19849..20730
                     /locus_tag="OUB02_001603"
     CDS             19849..20730
                     /locus_tag="OUB02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCT8885366.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8355 a         2425 c         3851 g         6332 t
ORIGIN      
        1 aatatttata ttagttttta tacatacttg gtataagttt ttcatatatt gattaaaatt
       61 gatgttgatt tattattttt agtatttttt caccttttga ttcatacttt gtagaatggt
      121 gtattttcta aaactgatta taagacaagt tcattcaaat tagcattttt atatcatata
      181 aataattgtt ccccctaaat aagatttaga tgtatagtat taaaatcaaa aagtataaaa
      241 taaaattgat gcttaatcat agataaatat taataaaata tacaagggga ttgtctaata
      301 aaaagataat ctcctttttt acaaataatt ctaaattgtg atataaatct aaaaatttta
      361 tcttaacatt taaccattta atgataataa ttgatataat aaatataaat taaatatatg
      421 gtgtacttat tttatgtttt tagcaagaaa tatagcattt aaaagataaa ttgtgcaact
      481 taaatcaaag tttaagatga actattaaca tagatatatt aaaaactttt gtaatggaga
      541 atatatgtga tatgaataat aaagttaagg ttgcagtatt agatacagga atagacaaag
      601 aacatgatta cttaaaagat aatttagttg gaggaattgc ttttgaatgt atgcatgatt
      661 atatctttat atcagataaa tttgatgatg aagatggtca tggaactgct tgtgcatcaa
      721 taataaagaa agagtatgaa gatgtagagt tatttgtaat aaaaattttg agaaaagatg
      781 gtattacaaa tataaaagtt cttgaagaag cattaagata ccttttagac actaacataa
      841 gattaattaa tttaagttta tcagtcatag gagtagaaag tgttaagggc ttatttgaag
      901 tttgttatga attgtttaga aaaggaaaaa taatagtatg ttcactcgct aatgattttg
      961 acttaagtta tccagctatg tttaacaatg ttataggtgt aagaggattt actttagatg
     1021 gagatgattc attttggtac aacaaaaaat atgatgttca atgtgttatg gatagcaatt
     1081 catatataag ttgtgatata aataattcct atagattacc accaaaatgt aatagttatt
     1141 tatcagcaaa gcttactggt aaaatagcta agatattatc agaaaaacca ggcataacaa
     1201 tatctgattt aaatgataag cttgaatcat tagcaactag aaatcattgg gatagttgtg
     1261 attttgatga gtgtagtaga attgtaaact ttaaattgga tttgtatgac aaagaaaatg
     1321 ctcttcttat gaaagttgct gacgttgtta gggattgctt agatattgaa gataataatg
     1381 aaaaactctt taagggtagc ctttttaata aggagatagg cttggtttat gataattgtt
     1441 ttaatctgtt gaaaaagcta gagcatagat ttgacataaa gtttaattat atggacatct
     1501 caaaatatga cttagtttca atatatacac tgactgaatt agtggaaaga tataaaaact
     1561 tataaataaa gatttgtagc taaatttaat gtagaaggta ttaaattata tgtattcttt
     1621 gtcggtagat taaaagtaaa aaatatacta taattagata gcataagaga aaatgccaat
     1681 acagggggag ttagataatg aaatatgaag aagcattaga gtacatatca caaactaata
     1741 aattcggtat aagattagga ttagaaaata tcggaaagtt attagaatta ttaggaaatc
     1801 cacaagaaac tttgaatata atacatgtag ctggaactaa tggaaaaggt tctgtgtgtt
     1861 cttttgtttc taatatacta agagagtgtg gatataaagt tggtttatat acatctccat
     1921 atttagaaac ttttactgag cgtataagag taaatggaca aaacatacca caggatgatg
     1981 ttgctagaat aattgagtta ataaaagaaa aaatagagat tatggtaaaa gaaggctatg
     2041 catatccaac agaatttgaa gtggttactg caatggcatt ttattactat tcagaacaaa
     2101 aggtagactt tgtggcatta gaagttggtt taggtggcag atatgatgct acaaatataa
     2161 taacaaaatc acttgtaagt gtgatagtat ctataagttt ggaccataca ggtatacttg
     2221 gagatacaat agaaaaaata gcgtacgaaa aggctggaat aataaaggaa aatggagtag
     2281 tactagtata tgaccagact gatgaagcaa aggatgttat aaagtcagtt tgtaaagaga
     2341 aaaaagctaa atatatagaa gttgattttg atgatataaa cataaaaaaa tcagatataa
     2401 attctcaaat atacgattgt actgtaatga aagaaactta tagagacctt gaaataaaat
     2461 tgataggcga acatcaaata aacaattcta tcttggcaat tagtgtaata aaatatctaa
     2521 aagacataaa taaattagca aatataaatg aagaatctat aagaaaagga cttataaata
     2581 ctaaatggcc aggaagaata gaaaagataa aggaaaatcc aatttttata atagatggtg
     2641 cacataatga agatggagca aaatctttgg caaaagcact tgataagaat tttaaaggta
     2701 gaaaattaac cttattaata ggtatgttag aagataaaga tattgatagt gttttagaga
     2761 tattgttgtc acattttaat aaggtaataa caacaacacc aaataaccca agggccataa
     2821 attcagatat attaagagaa aaagtattaa aatatgtaga tgatgtaaca tcaaagcatg
     2881 agatagagga tgcagtaaat tatacattag aaacatcaag tgaggatgat attataataa
     2941 gtgcaggttc attatatatg attggtacag tgagaacttt agtaaagaaa ttataatttt
     3001 ataaaataag tatacaaata aaaatataaa aaagagttgt ctaaaaaatg gacaactctt
     3061 tttatataaa ataagttttt aataatttta ctgatgttta tataagtaat tatatagatt
     3121 gacgtatagc tttagataat tggtctgggc aagaagtacc ttttccacca cagtcaatac
     3181 catttaattt atcagcaact tcaagagcat tttggccaat tattaagctt ctaagtcctg
     3241 ataaattacc attacatcct cctataaact cagcatcaac aatcacatta ttttcatcaa
     3301 cttcaaaaat catttctcta caacatacac cacttggatt aaatgttact ttcattaaaa
     3361 agacctccat taaatattat ttcttttttt aacattaata ataataccaa atatatgcgt
     3421 aataatgcaa ctataaagca taatacaagc taatgaaata atatattatt gtagtatatg
     3481 tattgtagag aggaggggtt atatgaaaat ctatatttct aactacttat caaagagtat
     3541 tagcatagtt gattattcta cacttgaact agaaaaagaa atagtattag aagataatat
     3601 atatccacac catttttgca tagaaaaaga aaaaaattta atatacatac ctagttcaag
     3661 taatgggata ctctatgtct tagatttaag taatgataaa ataattgata ctgtctctat
     3721 aggaggaagt cttagtcaag ttgtacttag cgataatgaa ttgtttgtgg caaatgaaga
     3781 ttctaatagt atatatgtac tagataaaaa cacattaaat ccaataggaa ttattggagt
     3841 tgataatatg ccacatggat ttgactttga tagagaaagc aaaaagttat atgtaccgtg
     3901 tataaactcg atagtatgta tagatacaat taataaaaat atacaaaaga aaataaatat
     3961 ggactttaga gcttggcata tagaacttga taaacaaaag aaagaaatgt atatatcaac
     4021 acttgatgga aagcttataa taatagatga agtaagcatg gatataaaaa aggtattata
     4081 tgaattttta ttaccagtag aaataagatt taattatagt ggcaaaaaag tttatgttgc
     4141 tgatttgggt tataataatg taagaatttt agattataca acgagtagat acattggaaa
     4201 tattgaaata aatgggattc cccaaggatt agaaatatct aaagatgaaa agttactatt
     4261 tgtatcagat acacaagaaa attcagtaaa agtttatgaa actgcaaata acaaattaat
     4321 aaaagaaatc aaggtaggaa gagagcctac aactataatt tgtttgtagg ctttaatgta
     4381 tgtctcttat aagtaaatcg ataacttcgg catacatata agatgcatta tttttccagt
     4441 tattgcaaat aagttttgct tgtttctctg atgaaacatt tagatttaaa tctattaagt
     4501 tagactgatt ttctatgact ctaaggttga caataaattc attatcactt tgttttacaa
     4561 aactagactt tacttgagta tctgctagta gattttcttt gttagaatct atatacttgt
     4621 ctattttttc agtaacacta gtaggtattc gattaaagaa gtattctaga ctttctacgc
     4681 ctctttgagt aagagcataa tattctctgt cagagtcttt atatgtagtc aaaaacttag
     4741 attccataag ctgagataaa agctgttgta atgaaaaata attcatcatc tcagtctcta
     4801 atactacttg agttatttga gaattagtta aatccatttt aattttttct aatatatata
     4861 aaattaaaag tttgtgatag gctaattctt cagacgagtt ttcaaacata aatatcatcc
     4921 tccgtcaaac tatttctata tataatatat taaactaatt tgtacaaaat taaaagaaca
     4981 atttgtattg tattattaat ctttaataat tcatataaaa acaattttat aaataaatat
     5041 agagaaacag ggctgctaat tagcagccct gttgataccc aggtgtcaaa atttactatt
     5101 tattttccag ccatttgtct ttcagccatt tcaactaatt tcttagtcat atatccacca
     5161 acatatccat tttctctagc tgttaagtta cctttgtcta tgttttcata gttagataaa
     5221 cctatttcgt tagctatttc taatttcatt tggtttaaag cagcttttgc ttctggaact
     5281 actgttctgt ttgaatttga catagtaaat ttcctcctca aataattagt ttatgatgtc
     5341 taacaacact tttatttatg tgatgttaat actaatttct ccaaaataaa aataatatat
     5401 acagaaaaat aaaggtaaaa atgtaaaaaa tataaaaaaa ttaaataaat gagtatttta
     5461 gagagtttaa ttaggtcaat ataggtttta tttttttagt tattgtctta taaattaaat
     5521 attacagtac ttttttgagc tgaaaaaaaa taaattttaa atatagaaga aattatttca
     5581 aagggggaat agtatgaaaa gagaagttaa aaggactaaa gctttgatat atagtgtttt
     5641 atttttttca ttattattag tgttaatatt tgtttttgtt tatggaataa aatacatatg
     5701 ttctaaatct gacaattcaa aaatgcctat atataaagtt gatacaaatg agaaaaaaat
     5761 agctttgagt tttgatgtag catggggaac tgataatatg gatgatattt taaaaatttt
     5821 agataaacat aatgtaaaag ctaccttctt tttagttggt agttgggtgg atgataatga
     5881 agaaattgta aaagctatag atgaaaaagg acatgaaatt ggaaatcatt caaatactca
     5941 tgccaatttg aaggaaatat caaaagaaga cataacaaaa gagatagaaa ctacatcaga
     6001 aaaaatatat aatataacag gaaaaaggac taatttattt agacctcctt ttggggacgt
     6061 aaacaataaa gctatggaca tttgtagtga tttaggttat aaagtaatta agtgggatgt
     6121 agattctata gattggaagg agcttggacc aaatcatgta attgagaaag taataaaaga
     6181 atctcaacct ggttcaatag ttttattcca tgctaatata aatgatgtag ataactattt
     6241 ggatactata ataagtcgac taaaaaaaga tggatacagt ctagtcaaaa tatctgattt
     6301 gttgtataaa gataattatg tagttgattc caatggagtg caaaaaacta agaattaata
     6361 agtaataaat gtatatttta agtaaagttg tgataaatta tatagtatat gttagaaaag
     6421 tgtcgataaa aagacggtgt ctattttttg acacttttta tacatgtatg caatatcaat
     6481 aattatagat taaattgatt aaaaaagtgt ctaataattg actttttttt gatgggttta
     6541 aaaatttaga agaattaatg aatgctgtaa gacagtattt ccaaagggta taagtttgtt
     6601 taaaagttgg catagaaatt gctttgttaa taagcgatat gttctataga aataaacaat
     6661 atgttaagac cttataatag aaaaatagca ttaacattat ttgaacagct tataaaaaaa
     6721 ttaatatact taagggggcg tactagttga acaaactata ttctatttta ttaaaacaac
     6781 acgttggtgg tccagataag ccagtagtaa gtgttggaga tgtagtaaaa aaaggaactc
     6841 taatcgcaga accaactgga ttaggggcaa atatttatgc cagtgttagt ggaaaaataa
     6901 gtgaaataaa tgaccaagcg atagtaattg aagctgatga agtacaagaa gatacttttg
     6961 aaccattaaa aggagaaggt atacttgact taattaaaga agctggagta gtaggaatgg
     7021 gtggagcagg attccctact catgtaaaat taaacataga tttaaatggt ggaacaatat
     7081 tagcaaatgc agctgaatgt gaacctttat tagctcataa tataaaagaa atagaagaaa
     7141 gaccagaaat agtttatcaa ggtataaaat atgctatgga agttactaat gctggaaaag
     7201 gtatgctagc aataaaaagt aagcatccaa aagcaataga agctttcaaa aaagtaataa
     7261 agccaggaga caacatagaa gttgcagaat tagttgatat gtacccaatg ggagaagaga
     7321 gagcaatagt aagagacgtt ctaggaaaat tacttgagcc aactcaatta ccttcagagg
     7381 ctaatgcagt agtaataaac gtagaaacac taactagaat agtagaggct gttgagcaaa
     7441 aaagaccagt tatatctaaa aatataactg tagttggtca attaaacagt ggaaaagaat
     7501 ctatagtgtt tgaagatgtg ccaattggaa caactgtcgg agaattaatt gaaagagctg
     7561 gtggaataaa aggtgaatat ggtgaaataa tcctaggagg acctttcact ggtaaagcta
     7621 ctactttaga tgctccaata actaaaacta gtggtggtat aatagttact atgccatttg
     7681 ttaatgaaaa gagaaaaatg ggtcttttag tttgtgcatg tggacctaat gaagaaagaa
     7741 tgagagatat agcaactaaa atgggagtta ctgatatagt atcagttcaa aaatgtaaac
     7801 aagctcaaga aattaaaggt gcattaaaat gtgaaaatcc aggacactgt ccaggacaag
     7861 ctcaaaaatg tattgaattt aagaaagctg gagcagaagt tatattaata ggaaactgta
     7921 ctgactgtag taacacagta atgggttctg caccaaaatt aaaattaggt gtatatcata
     7981 ttactgacca tgtaatgaga acagttaatc acccattaat aagaagaata aaaggtgacg
     8041 taagattaaa aataaaataa tagataaata gaagttagac atctttagaa gtaaaactta
     8101 aaagttactt ctaaggatgt tttttaatgt attttgaggg caatatatat attgtaaaat
     8161 aaatggaaaa aatagacaaa atatgctaga tattgtataa tgataacaat agctattaca
     8221 attaatattg gacaaaaata atgatactca agtatgtggt ttgtattaat atgcatatta
     8281 tattaagtca actatccgat agagtaaaag gtttttgtga tatttgaaga tactctatag
     8341 tatctttttt attataaaaa ctaatttttg ttttttatta gataagtatc taggatacta
     8401 atttatattt tattgagttg ttaaaaaagt gcacttaaaa agttaaaaaa gaaaaattaa
     8461 aaacctattt atagaaactg tgaagtatat cttaaacctg ggcacttaaa agatatatgg
     8521 agttagtagt gcaacctgct ataaatataa ataggagtaa cttttaattg tcaaaaatat
     8581 ataaaataaa tacataaata agtaattgat agaagataga tataaatata tttttgaaat
     8641 gctccctgca ttcataacga gtgtagagtg gcagatatct gcccaaaact cctttaattg
     8701 ctggaagttc ctaaagctaa ctaaaccaca acataatcat gaaataagga taagtgtgat
     8761 agagtgtgca tttaataagc acttgaggtt acgaaagtag aaaaaattag ttagatagca
     8821 taaggttaaa tcctaagtgc cttgaaaatg gataatcagc aggtaagctt cgaaaagaag
     8881 aaacttcaac gactagagag aaatctcgta cctacaagtg tggggaaatg gggagtacct
     8941 aatttatggt taggtaatga gatagtctgt gcttaataga aatattaaga agttcatagg
     9001 agaactggtt aggagtaacg accctaattg aacacactaa agatgtatta ttgaataaga
     9061 ttcatataat atttaataat tttaattata ttaagtgaat acaatatatg tttttagtat
     9121 caggagaatt ttgaaaacaa aaattaaaaa atcaagtata atcagtttag taattgcttt
     9181 tagtatggta tttactgcat ttacaccaat agtatcttat gctgacgaag tgactagcaa
     9241 tgatacaata cttaatggag aagaacaatc aaatggtcag acaccagatg tagagaaacc
     9301 atcagacggt caggtaccag atggggagaa accatcagac agtcaagtac cagatgggga
     9361 gaaaccatca gacggtcagg taccagatgg ggagaaacca tcagatggtc agataccaga
     9421 tggagagaaa ccatcagatg gtcagatacc agatggagag aaaccatcag atggtcagat
     9481 accagatgga gagaagccat cagatagtca gacgccagat gtggagaaac catcagatgg
     9541 tcagatgcca gatgtggaga aaccattaga tgagcaaact ccagaagaag aaaaaccatt
     9601 ggaagaaaaa atagttatag aggaaatgag tttaaaacaa gatattgata aaattcttga
     9661 tatgacttta tctcaaataa ataaaatagt atataacttt tgggaagatg aagaagatgt
     9721 aaaggcagat gaacaatcag aaattaacca agtttttact tcaaaagact catttattag
     9781 cttatggtat gacaaaaaag caaaagtaaa aaattcttgt ttactaaaag aaatagatgg
     9841 ttcagataga ccttatcatg atttaagatt tgatgatatt acaaagactt taacttttga
     9901 ctatgtaata aagggtcttg taggagcaag ctcaaaagac gacaaatatg taatagaagg
     9961 tgaagatgga gagcaaactg cattttgtta caataatcat ttaagaccac caagtagtaa
    10021 tggaaagagt ccatatttac cagcagaaga ttttaatggt caagaaaacc aaaatgaaga
    10081 agcagttaaa tctatactat atgcaggttc tgaatttgat ggatttggat ataagcaaca
    10141 atttaattta ggtggagaag aaaatgaatt gatgacttac tcagctactc aaagtgcaat
    10201 ttggattatt cttggacaaa tggatgaaca agaaagactt aagcagtatc aaggttctat
    10261 aaacctttgt gataaactta tagaaagagc aaaaactgaa gaggaaagag cggagtatca
    10321 gaagaggaaa gaagtagcga taaatacaaa agcttattta gaagcattgt taaaagcagg
    10381 tcgtgaagag ttaaagccaa atgatacagg aaaacctagt ttgagtaatg gtttaactaa
    10441 gattaacttt gaaaaaaatg aagatggtac ttatgaaaca gaagctgtag ccttagtagg
    10501 ttatagtggt gtagtaaagt taaaactacc agatggagta actgcttatg atgtagatgg
    10561 taatataata ggtacaggcg aagtagaaat ttcaacgcaa cagaaattta aacttaagag
    10621 tgttggaaaa ccagatgcca aagctaatat ttctgctgta tcttatgatt atatttttcc
    10681 aaaagctatt caatattata aggcagtgct tgatttagga caaaaagacc attcatcttc
    10741 attaccatct agcaagcaaa atttattaag ttatacaata gagaaaaaga atggtcaaga
    10801 agttaacttt aatataggtt taccaacaga tgatgatgtt gtaaatccac cagtgccacc
    10861 aatagatgat gatgttgtaa atccaccggt gccaccaaca gatgatacaa atggacataa
    10921 gccaaaacca tcaccaccaa tagatgatac agtaataaat ccaccagtac caccaatgga
    10981 tgatacaata ataaatccac cagtaccacc aacggaggat gcagtgttaa atccaccagt
    11041 gccaccaatg gaggatgcag tgctaaatcc accagtgcca ccaacagatg atacagtgtt
    11101 aaatccacca gtaccatcag taagtgatac agtggaaaaa actccagaac tatcaagaga
    11161 tgatacaata ataaaatcac caaaaacagg tgatgaaact cagctaatgt catatgtatt
    11221 tattagtgtc attgcaattt gtggattagc gtatcaatgt aaaataaaaa gaaattaatc
    11281 tagattatta ttaagtttaa aataaaaagt ctcaaattga tttataagtc attttggggc
    11341 ttttttatgt aaatataata tgcaaatata gtttgtttaa aaatatctat cttaattata
    11401 ttcttaaatt tatgattgga tacaggcatt cattaattgt ttgatgaata gaaaaatgtt
    11461 ttgaaggtga aaatagtatt gaaaaaataa tatactaagg gtataattca tttgatttca
    11521 tttgtataaa tttaattata aattttaaag tgattatata aatttaaatg ctaattactt
    11581 aataaatttt aataataatt atttaataaa taattattat aatatgctag aataaatata
    11641 tataaataag ttttgatgag gtactactat aaagggggga ctttatgttt tcgataccag
    11701 aagtaaaaaa agtagaagag gtaatggata caaaatttac aactatagac gaagacacaa
    11761 gaattgaaga tgcaataaaa gaaatgataa aaagcaacac aaaaactcta atggtaatag
    11821 attcatctga ccaattaaaa ggcattatat ccatgacgga tatacataat ctttatgaga
    11881 tgcataaaaa gtatgaaggt caaccagtaa aacttataat gaagaaggat gtaatatatg
    11941 tcaacgaagg tttaacctta gatgaatgta gagatataat gatattaaaa aatataggga
    12001 ttcttcctgt tcttagagat aataaaatta ttggagttct aaaacaagag cacattaggg
    12061 actatctata tatgcattta gaagattatg gattaacttt gaagtatatt ataggtcaaa
    12121 ttaaagaagg aatttgtgct ataaataacg aaggtgttgt tatcttgtgg aataagttta
    12181 tggaggaaag gtatgatata aagtctgaag atatagttgg tcgaccgatg aatgaatttt
    12241 tggaaaatac tatttcagaa aaagtactaa atagtaaagt tggaatgagt gacttgtatt
    12301 ttacagataa aaaagaaaat atgtatgcat tagttcatgc aaatcctata ttttataaag
    12361 aagaatttat cggtgttgta tgtacagaag ttgatgttac agaagctaag atattagctc
    12421 ttgaacttga aaaagtaaat gatacattaa aatatctaaa gaacgaagtc aaaaatttat
    12481 ctaaaggtag ttttgataag attttaggta aaagttataa attggaaaaa tcgaaagcga
    12541 ttgcaaaaca agtggctaga acaaatagta gcatatttat ttggggcgaa agtggtacag
    12601 gtaaagaagt atttgcaaga gctattcatg attatagtga gagaaaaggt caatttatac
    12661 ctgttaactg tagtgccata ccaaatgagt tatttgaaag tgagtttttt gggtatgaat
    12721 caggtgcgtt tacaggagca agtaaaaagg gtcgaatagg tatatttgaa cttgcaaaag
    12781 atggaactgt atttttagat gaaattgccg atttacctct tagtatgcaa gcaaagttgc
    12841 ttagagtatt acaggaaaaa gaaataagaa gagttggtgg agataccaca ataaaaataa
    12901 atcctagaat aatatcagct accaataaag atttgagtaa aatggtaaag gcagaaaaat
    12961 ttagagaaga tttatactat agattaaatg ttgttgaaat aaagattcct ccacttagag
    13021 agagaaaaga agacatagga ttattggttc atagcttttt agaggagata tgtaaacaaa
    13081 ataataagcc tgttcttaca attagcaaag atgtaataga tatatttcag aattatagat
    13141 ggaaaggtaa tattagagaa ttaaaaaata cgattgaaaa tattgttgta ttgtcacaaa
    13201 attctaaaat agatgtggat gatgtaccaa gttatatgat ggattctaca aataatagta
    13261 ctgaagaaga agagtatcct ttagatttaa cgaaggcaac tcaaaaaatt gaaataaaga
    13321 atataaccaa ggcacttaaa atgtctaatg gaaataaagc taaagcagct aagatattaa
    13381 atatacccag aacaactttg tattataaga tagaccaata caaaatagat gtgtctaaaa
    13441 tatgacacat gactaaattt tgacaaaatg ataaacttta atttaaaacc tagtaaagcc
    13501 aacgcttgta taagtgttgt gtatatagat gtgttaaaaa aaatgaaaaa gtgttgatta
    13561 attgacactt tttttgtttg agaaaatgtt tacaaataca aaaaataaca tattttaaat
    13621 aaatatttat ataatatttg gctaaaaata gcgatagatg aagaatctat tatagaaaaa
    13681 atctttttgt taataagttg gcataggaat tgcttataat ataaagtgac tttaagtgag
    13741 aaaaaaataa tattaatcat aacaaattat gtaggtttta atttacttaa tttctcagtt
    13801 aaggggttac ctgttgagga attgagattt aaaataataa ataagaaaat aatgtaagga
    13861 ggtattttat actatgtcaa taactttaga aacagctcaa gcccatgcaa atgacccagc
    13921 agtttgttgt tgtagatttg aagcgggaac aattatagcg ccagaaaact tagaagatcc
    13981 agcaatattt gcagacttag aggattctgg attattaaca ataccagaaa atggattaac
    14041 tataggtcaa gtactaggag ctaagttaaa agaaacttta gatgcacttt ctccaatgac
    14101 tacagataac gtagaaggat acaaagcagg agaggctaaa gaagaagtag tagaagaaac
    14161 agtagaagaa gcagctccag tatcagaagc agcagtagtt ccagtaagca caggagttgc
    14221 aggtgaaaca gttaaaatac acataggtga aggtaagaac ataagcttag agataccttt
    14281 atcagtagct ggtcaagcag gagttgctgc tccagtagca aacgttgctg ctccagtggc
    14341 aagtgcagca gcagaagtag ctccaaaagt tgaagaaaag aaacttttaa gaagcttaac
    14401 taaaaaacac tttaaaatag ataaagttga atttgctgat gaaactaaaa tagaaggaac
    14461 tactttatac atcagaaacg cagaagaaat atgtaaagaa gctaatgaaa ctcaagagtt
    14521 agttgtagat atgaagttag aaataataac tcctgataaa tatgaaactt acagtgaagc
    14581 tgtattagat atacaaccaa tcgctactaa agaagaaggc gaattaggtt caggtataac
    14641 tagagttata gatggagctg taatggtatt aactggtaca gatgaagatg gagttcaaat
    14701 aggtgaattt ggttcttcag aaggtgagtt aaatactact ataatgtggg gtagaccagg
    14761 tgctgctgac aaaggtgaaa tattcatcaa aggtcaagta acaataaaag caggaactaa
    14821 catggaaaga ccaggacctt tagctgctca ccgtgcattt gactatgtaa ctcaagaaat
    14881 aagagaagca ttaaagaaag ttgacaactc tttagtagtt gatgaagaag taatagagca
    14941 atacagaaga gaaggtaaaa agaaagttgt tgttataaaa gaaataatgg gacaaggtgc
    15001 aatgcatgat aacctaatat taccagttga gccagttggt acattaggag ctcaaccaaa
    15061 cgttgactta ggaaacatgc cagttgtatt atctccactt gaagtattag atggtggtat
    15121 ccatgcatta acttgtatag gacctgcatc aaaagaaatg tcaagacatt actggagaga
    15181 gccattagta ataagagcta tggaagacga agaaatagat ttagtaggtg ttgtatttgt
    15241 tggttctcca caagtaaatg ctgagaaatt ctatgtatct aagagattag gtatgttagt
    15301 tgaagctatg gaagttgatg gagctgtagt aactactgaa ggtttcggaa acaaccatat
    15361 agatttcgca tctcacatag agcaaatagg tatgagaggt ataccagtag ttggtgtaag
    15421 tttctcagct gttcaaggtg ctctagttgt tggtaataaa tacatgactc acatggtaga
    15481 caacaataag tctaagcaag gtatagagaa tgaaatatta tctaacaaca ctttagctcc
    15541 agaagatgct gttagaataa tggctatgct taaaaatgct atagaaggtg tagaagttaa
    15601 agctcctgaa agaaaatgga atccaaatgt taaattaaat aacatagaag ctatagaaaa
    15661 agttacagga gaaaaaatag tattagaaga gaatgagcaa tctctaccaa tgagtaagaa
    15721 gagaagagaa atatacgaaa aagacgaaaa ctaaaattta gatactaact aagatttaga
    15781 caataacata aatttagatc caagtttaaa acagtataga gaggtagaat tataatggat
    15841 aggttaaaat atatctcatc agagactttt tatgaaggta ttatagttga catcaaaggt
    15901 ggtggagtga ctatagacct aaaaggtaga cttggacaat ttaaaatacc taatagaatg
    15961 cttataactg actacgaact taagataggt caagaagttg gattcatgct aagctatcct
    16021 gaggtattaa gtccagagcc taatcaagag tatgtagaaa atattaggag agaaaaagaa
    16081 aaacaagcag aaatgcaatc aaaacaagaa gaaaaataat aaataatcgt ttgaaaatag
    16141 aagggagagg aaatatatga gccttacaac agtacaagga cttcaatctg aaatattcgt
    16201 tccaataaca cctcctccag tttggactcc tgtaactaaa gaattaaaag atatgactat
    16261 agctttagct acagcagctg gtgttcattt aaaatctgac aagagattta atctagcagg
    16321 agatactaca tttagagcta tacctaatac agctacagtt gatgaaatga tggtatcaca
    16381 tggtggatat gacaatggag atgtaaacaa agatataaac tgtatgttcc ctatagatag
    16441 attacatgaa ttagctgcag aaggatttat caaaggagta gctcctatgc actatgcatt
    16501 catgggtggt ggaggaaacc aacatgtctt cactgaagaa actggtcctg ctatcgctgc
    16561 taaacttaag gaagagggag tagacggtgt agttatgaca gctggctgag gtacttgcca
    16621 tagaactgcc gtgatcgtgc agagagcaat agaggaagct ggaataccta caataataat
    16681 agcagctctt cctccagtag ttagacaaaa cggaactcct agagcagttg ctccattagt
    16741 tccaatgggt gctaatgctg gtgaaccaca taatatagaa atgcaaacac atatattaag
    16801 agatacatta gagcaattag ttgcaatacc atctgctggt aagatagttc cattaccata
    16861 cgaatataaa gctcacgttt aaaatgtaat ttatatttga aaatatagaa cccttctcct
    16921 acacggttag gagaggggtt ttaagaacaa aggtggatat tatggaagaa aaaataatga
    16981 gaagacttgt tataaaaccg tttcatatga atgaagttaa tttcggttca aaaacttcta
    17041 taaagaaaga tgtacttaca atagatttat caagtataga tgaaataaaa gaaagagaag
    17101 agttaataac agacataaaa gtagacatta taaaacctgg agattatgac agagaaatta
    17161 atactattat ggacattata ccaatatcaa caaaagtttt aggcagactg ggagagggta
    17221 ttacacatac tttaacgggt gtatatgtaa tgttaactgg tgcagatgaa gatggaaatc
    17281 aaatgcatga atttggttct tcagaaggaa ttttgaaaga ccaaatggta tttgggagat
    17341 atggaactcc atcagttgaa gattatataa tccatgtaga tgtaacttta aagggaggtt
    17401 taccttttga aagaacactt ccacttgcag cattcagagc atgtgatgat tttatacaag
    17461 aaataagagc gtctttgaaa atggaagacg gaagaaatgc tactcaagtt cgtgaatatt
    17521 ttgacaaaat tcgaccaaat gcaaaaaagg ttgtcatagt aaaacaaata gcaggtcaag
    17581 gtgctatgta tgacaatcag ttattctcaa aggaacctag tggatttgaa ggaggaacat
    17641 caattataga tatgggaaat gtacctatga ttatatctcc taacgaatac agagatggtg
    17701 cccttagagc tatgacttaa gagaggtgga tattatggga ataggacctt caacaaaaga
    17761 aacatctctt catcacttta gagacccact tttagacata gttgaaagtg ataaggatgt
    17821 agatttactt ggagtaatag tagtaggaac tccagatgga aatgaaaata aaacctttgt
    17881 gggacaaaga acggccgctt ggctggaagc catgagagta gatggagcta tagtttcatc
    17941 agatggttgg ggaaactcac atgttgacta cgctaacaca tttgaggaaa taggaaaaag
    18001 agatatacct gtagttggag ttacatttaa tgggactcaa gctaagtttg ttgtaagcaa
    18061 tcaatatatg gataccatag ttgatatgaa caaatcaaaa gaaggtattg agacagaagt
    18121 agttggagaa aacaacacaa atgaaataga tgctaaaaaa gctttggcat ttttaaaact
    18181 taaaatgaga aaacatggat aaaaaattta agtaagggaa tgagttttgt gggaatttct
    18241 acatcaatga aaaacacatc acttcatata tttagagacc cgttactaga tttgatagat
    18301 aatgattctg acatagactt gttaggagtg gttgtagttg ggacatcaga gcacaatgaa
    18361 tggaaaacat ttttagcaac tagacttgga agatggattg aatcattgcg acctgatgga
    18421 gtgattataa ctttagatat ggcaggaaac caacatattg attttacaaa tgctatagca
    18481 gagtttgtaa aatcagatat acctacagta ggtcttacta ttatgggagc agatggatta
    18541 gtaattacca atccatattt ggataaagct accataattg attataaaaa gacaacaggc
    18601 tacatagaaa cagaagtagt tggagacaat cacatggatg aagttgatgt taaaaaagca
    18661 ttggctttcc taaaattaaa aatgcgtaaa gacgcaaaat aaacctaagg gggaatttga
    18721 tatgaaattt agcagaagta tacaagctat agactctcat acagcaggtg aagcaactag
    18781 aatagttgta ggtggaatac ctaacataaa aggaaattct atgcctgaga aaaaagaata
    18841 tttagaggaa aatctagatt atttaagaac tgctataatg ttagagccaa gaggacataa
    18901 tgatatgttt ggttctgtaa tgactcaacc ttgttgccca gatgctgact ttggaataat
    18961 cttcatggat ggtggcggat accttaacat gtgtggccat ggtacaatag gagctatgac
    19021 agcagctata gaaacaggtg tagttccagc agtagagcct gtaactcatg tagttatgga
    19081 agctccagca ggaataataa gaggagacgt tacagttgta gatggaaaag ctaaagaagt
    19141 atcattctta aacgtaccag ctttcttata taaagaaggt gttgaagttg acttaccagg
    19201 tgttggaact gttaaatttg acatatcatt tggtggaagc ttctttgcta taatacatgc
    19261 aagtcaatta ggtttaaaaa tcgaacctca aaatgctggt aaattaactg aactagctat
    19321 gaaacttaga gatataataa atgagaaaat agaaatacaa catccaactt tagctcatat
    19381 aaaaactgta gacttagttg aaatatatga tgagccaact catccagaag ctacttacaa
    19441 aaatgtagtt atatttggtc aaggtcaagt tgacagatct ccatgtggaa ctggaacaag
    19501 tgctaaatta gctactttac atgctaaagg tgaattaaaa gtaggagaaa aattcgtata
    19561 tgaaagtata ttaggaactt tattcaaagg tgaaatagtt gaagaaacta aagttgcaga
    19621 tttcaatgct gtagtaccta aaataactgg ttctgcttat ataactggat ttaatcattt
    19681 tgtaatagat gaagaagacc cacttaaaca tggatttatt cttaaataag taataaataa
    19741 attaggaaat taaaatagaa tagtagccac ttgtcggttg ctattctata gcttttaggc
    19801 tataagattc agaatatatg aaaaaataaa aaaataggag gatggattat ggtagcaatc
    19861 ttaaaggcat tatatgtagt attcgcagca atctttgcag ttgtgtttgg taaagacgta
    19921 gctaaagcaa acaaagaggg gaaactagaa gaacaaagta ctccaaaagt tgctgtaaca
    19981 ggatttataa cagacttctt tgatacatta ggaatagggt catttgctcc aacaatagca
    20041 atgtcaaagg ctttaaagtt aaatatacca gataaaaaaa tgcctggtac attaaacgta
    20101 gcacatacta ttccagttgt tacagaagca ttcatattta catcaatcat accagttgat
    20161 ggtgttactt tagtatctat ggttgtagca gcagcagttg gttcatatat aggtgcagga
    20221 ataatagcta agatggacga gagaaaaata caacttgtta tgggtgtaac tttagcatta
    20281 acagcaatat taatgttatt aggtcatcct tggattaacg tattaccagg tggaggaaat
    20341 gaattaggtc ttactggtgt taaattagta ataggtgtaa taggtaactt tatactagga
    20401 gctttaatga cagctggtat aggtttatat gctccaggta tggcaatggt ttatttctta
    20461 ggaatgtctg caaaagttgc attcccaata gttatgggtt cttgtgcatt attaatgcca
    20521 gttgcaagta tgaaattcat aaaagaagat gcatatacta aaaaagcatc agttataata
    20581 gctatatgtg gtttaatagg agttttcgta gctgcatata tagtaaaaac tctaccaatg
    20641 gatatattaa aagcattagt aatagtagtt atagcttata cttctgctac aatgataatg
    20701 gctgcaaata aaaacaaaaa gactgcttag ttaaagacta cttagtgttt attaaatatt
    20761 acagctaata ataaagatta taaactgctt taaaatattg aattaatatt ttagggcagt
    20821 tttttattta ctaaattaat ataaagcaat taacatgcca attatgtaat atatatagtg
    20881 atttagtaga tttaatgtaa aaaacgttgt atttttaaag aataaagcat aatttaaagc
    20941 taaatattta aattttataa tca
//
LOCUS       DAKBNN010000050        20920 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000050 DAKBNN010000000
VERSION     DAKBNN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.050"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            101..1219
                     /locus_tag="OUB02_001604"
     CDS             101..1219
                     /locus_tag="OUB02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT8885367.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KSDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEVFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1337..1642
                     /locus_tag="OUB02_001605"
                     /pseudo
     CDS             1337..1642
                     /locus_tag="OUB02_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1655..2560
                     /gene="truB"
                     /locus_tag="OUB02_001606"
     CDS             1655..2560
                     /gene="truB"
                     /locus_tag="OUB02_001606"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCT8885368.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYCNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2691..3620
                     /locus_tag="OUB02_001607"
     CDS             2691..3620
                     /locus_tag="OUB02_001607"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCT8885369.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3724..3981
                     /gene="rpsO"
                     /locus_tag="OUB02_001608"
     CDS             3724..3981
                     /gene="rpsO"
                     /locus_tag="OUB02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCT8885370.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            4221..5066
                     /locus_tag="OUB02_001609"
     CDS             4221..5066
                     /locus_tag="OUB02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT8885371.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            5191..7302
                     /gene="pnp"
                     /locus_tag="OUB02_001610"
     CDS             5191..7302
                     /gene="pnp"
                     /locus_tag="OUB02_001610"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCT8885372.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            7568..8311
                     /locus_tag="OUB02_001611"
     CDS             7568..8311
                     /locus_tag="OUB02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8885373.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            8469..9716
                     /locus_tag="OUB02_001612"
     CDS             8469..9716
                     /locus_tag="OUB02_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCT8885374.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHINTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9751..9996
                     /locus_tag="OUB02_001613"
     CDS             9751..9996
                     /locus_tag="OUB02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HCT8885375.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            10006..11229
                     /gene="dapG"
                     /locus_tag="OUB02_001614"
     CDS             10006..11229
                     /gene="dapG"
                     /locus_tag="OUB02_001614"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCT8885376.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            11342..12076
                     /locus_tag="OUB02_001615"
     CDS             11342..12076
                     /locus_tag="OUB02_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HCT8885377.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            12199..14610
                     /locus_tag="OUB02_001616"
     CDS             12199..14610
                     /locus_tag="OUB02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCT8885378.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            14613..15671
                     /gene="mnmH"
                     /locus_tag="OUB02_001617"
     CDS             14613..15671
                     /gene="mnmH"
                     /locus_tag="OUB02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCT8885379.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15665..16999
                     /gene="rimO"
                     /locus_tag="OUB02_001618"
     CDS             15665..16999
                     /gene="rimO"
                     /locus_tag="OUB02_001618"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCT8885380.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16986..17528
                     /gene="pgsA"
                     /locus_tag="OUB02_001619"
     CDS             16986..17528
                     /gene="pgsA"
                     /locus_tag="OUB02_001619"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCT8885381.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17816..18862
                     /gene="recA"
                     /locus_tag="OUB02_001620"
     CDS             17816..18862
                     /gene="recA"
                     /locus_tag="OUB02_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCT8885382.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            19038..20579
                     /gene="rny"
                     /locus_tag="OUB02_001621"
     CDS             19038..20579
                     /gene="rny"
                     /locus_tag="OUB02_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCT8885383.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8592 a         2120 c         4003 g         6205 t
ORIGIN      
        1 attatgatag gtgattaata aattgttcac cacagcatta tataatatat taaatatgat
       61 aaaatcatta aaaagtgtat caaaaaacaa ttaacgtgag gtgatagcag tgaaaaaagc
      121 ctataagttt agaatgtatc caaataaaaa acaacaagag ttaattaaca aaacttttgg
      181 atgttgtaga tttgtatata ataaatatct tgctaaaaga atagatgttt ataaaagcga
      241 taaagaaact tttacttata aacagtgtag ttctgatttg accaatttaa aaaaagaatt
      301 aaaatggctt aaggaacctg acaaattttc actacaaaac gccttaaaag atttagataa
      361 tgcatataaa aaattcttta aagaaaaggc aggatttcct aaatttaaat caaagaaaat
      421 taatagattt tcatataaaa ctaactttac aaatggaaac attatgtatt gtggtcaata
      481 tataaaacta cctaaacttg gtatggttaa gataagggat aaacaagtac ctcaaggtag
      541 aatacttaat gctactatat ctaaagaacc aagtggtaga tattatgtat cattatgctg
      601 tactgatgta gatattgaag tatttgaaaa tactaataat catataggtt tagatttagg
      661 aattaaagaa ttttgtatct caagttgtgg agactttata gaaaatccta agtatcttaa
      721 gaagtcatta aacaagcttg ctaaattaca aagagaatta tcgagaaaaa caatcggtag
      781 tttaaataga aataaagcaa gattaaaagt tgcaggactt caagaacata tagctaatca
      841 aagaaatgat tttctacaaa aactatctac taaattaata aaagagaatg atattatttg
      901 catagaagat ttacaagtaa agaatatgat tagaaatcgt aaactatctc gcttaatttc
      961 agatgtatca tggtcagaat ttattcgtca attagaatac aaggctaatt ggtatggtag
     1021 aaaaattgta aaggtaggta aattctttgc aagttcacaa atatgcaata aatgtgggta
     1081 taaaaacgag gaagttaagg atttaaatat aagagaatgg atttgtccta gttgtaatga
     1141 aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat taagactaat
     1201 aacgattcaa aataaataac ttatgtaaga accgtaggaa ctacggggat agcctgtgga
     1261 gaattagtaa gacatatttt aatatgcaaa attctgtgaa gcaggaaccc tgtgacttta
     1321 gtcatgggag gttcagtcaa aggtatctag tatagtttta actcaagaca tgatgaatag
     1381 aaataatatt agctttaatg atgttgatgg aataacaaca tatacaagag acataaaagg
     1441 tatagaagtt ggaattttat ttaaagagaa aaaacaaaat gaaattaaag tgagttttag
     1501 gtcaaaaaat tatgttgatg taagtgaaat agccaagtta tttggcggtg gtggtcatgt
     1561 aagggctgct ggatgtacta ttagagatag tattgataat gcaaagaaaa tggtactaga
     1621 ggcagtgtta aaatcaattt aaggtgacag taaaatgaat aagattataa gtatattgaa
     1681 acccacaggt atgacatctc atgatgttgt aagtagagtg agaaagattt taaatataaa
     1741 aaaagttggt catacaggta ctcttgaccc agatgcttca ggtgttttac caatctgtat
     1801 agggaaagct acaaaagtat gtgaagttat tttaaacaaa gataaatcgt atatatgtga
     1861 gcttacttta ggtataagta cagatactta tgatgcttca ggtgaaattt taaaaaaagt
     1921 tgatgatttt aaatttagta atgaagacat agaaagagct tttgacacac aaagaggaga
     1981 gataaatcaa cttcctccaa tttattcagc tttaaaggta aatggaaaga gaatgtgtga
     2041 tttagtaaga agtggtagac aatctgaaat tactttaaaa acaagaagag taaatataaa
     2101 agacataaaa atactaagta taaaaggtaa taaagtaatg ttttatgttg agtgttctaa
     2161 aggtacttat gtgagaagta tttgccatga tataggagaa tatctaggtt gtggagctca
     2221 tatgtcgttt ttgaatagaa catcttctgg taaatttgac ttagataatt caataacatt
     2281 agaagaatta gaattatttt atgaaaataa aactttagac aaatacttat atgatataga
     2341 ttatgtcctt gattcgttta attatgttgt attaaaccct aatgctataa aatattattg
     2401 taatggtggc tcaatagatg ataaaaggtt tttaaaaaat aatttcgata aagatgatga
     2461 atttgtaaga gtatacagta cagataattt cttaggatta ggaaagttat caaaacacaa
     2521 taatacaata agtgttaaaa gtgataagat gttcatataa ccaatgtatt taaaaaaaca
     2581 acaaaagaga tagtactata ataacttcta tagtctatct cttttactta tgctataata
     2641 gtttgtgaat aagttaagag taaattatat tttagcgggg aacatttatt atggtgatta
     2701 taaaatctat aaaagaaata gcaaacataa aagaaagtgt cattaccata ggaaattttg
     2761 atggtgtaca caaaggacat caagttctta taggaaaaac agttgaacat gcaaaaaaag
     2821 aaaatataca gagtattgta tttacatttg caaatcatcc tgtaaattac tttagaccaa
     2881 actcaacaaa aaatattata agcaatgatg ataaaataaa attattagaa gatttgggaa
     2941 tagatatagt tgtaatcata ccatttgatg agtatatgac taaaatacct gcaaaggatt
     3001 tcgtagatga gatactagta aaaaaattaa aagctaaaaa gatagtaatt ggacatgact
     3061 ttacatttgc aagggcaaaa gaaggcaatg ttaatctatt aaaaagctta gaacatgaat
     3121 ttggttttga ggtagaagta ataaaaccca tcaaaatcaa tgatgtaaga gtgagtagta
     3181 cttatattag aagtttagta tcccagggtg atatggctaa tgtcaaggaa tatttgggta
     3241 gaaattacaa gttagaaggt tgtgttatac attctaaaca ccttggaaga actataggat
     3301 tcccaacggc aaatattgac cttaagaata atatgctcgt tccaaagaga ggtatttatg
     3361 cttctattgt acatattgga gatgagactt attttggtgc tacaaatgta ggctataacc
     3421 caacagttaa tggaaaaact ttgtctattg aaacaaatat tttagagttt gatagagata
     3481 tttatgggga aaatataatt gttgaatttt tggaaagaat aagagatgaa agaaagttta
     3541 attcaataga tgacttaaaa aatcaattgt ataatgatac aaattatgtg tatgaaaact
     3601 atgtttgcaa aaacaagtaa tgtatgataa aatgattaag gaaaataacc attactcggc
     3661 atttgaatac tccaatcaat gcttagtaat aggcgtttct aaaaaaatac ggaggtaatc
     3721 taaatgataa acaaatcaga aataataaaa gaatatggaa gagctgaagg ggatactggt
     3781 tctccagaag tacaaatagc tttattaaca gctagaataa acgagttaaa tggacattta
     3841 aaaactcata agaaagacca ccattcaaga agaggactat taaaaatggt tggtagaaga
     3901 agaaacttat tagcttactt aaaggaaaaa gatcttgaag gatatagagc attaatagct
     3961 aaattaggat taagaaagta atttcattct ttataaaaaa gcaggtatag tgtatacctg
     4021 cttttttata gttaattcat tataaagtaa aatagttaat aataaaagta gtttatatgt
     4081 tataaaaaat tataaaatga ataatattgg gtaagaatat taaaatagta tacaaataca
     4141 taatcaattt aaaaggaaag acaggataaa tatagaaatt ttataacagg ataattagtt
     4201 ggaataagga ggatattatt ttgaaaataa agttagtatg cgatagtttg tgcgatattc
     4261 cagatgaaat atcagaaaaa gattatatag agatagttcc acttacagtt atttttaatg
     4321 atagggaata tatagaagga gtagatataa agaaagaaga gttttataaa aaagtaaaag
     4381 aaatcaagca aatacctaaa acttcacaag ctacatatat ggagtttaaa gaagtgtttg
     4441 ataagttcat aacagaaggt tatcatataa tctgtatgac aggggctgca aacgcatcag
     4501 gaacttttca aagtgctatg atagctaaaa atgatgtaaa tgaaaatgaa aaaatacata
     4561 tatttgatac tagaaattta tctctaggta gtggacaata tgttataaag gcatgtgaac
     4621 ttcttgaaga aggactggga tttgaagaaa ttatagatga attagaaaat actcgcagta
     4681 gtgtaagatt gctttttgca ccatatacac ttgatttttt aaagcagagt ggaagagttc
     4741 cagttgcaac tgctttgata ggaaatatgt taaatataaa gcctatattt ttctttgata
     4801 atggggaagc taagttggta aataaggtta gaggaataaa gaatatagca agtaaattgg
     4861 tcgatataat tttagaaatg aatgaagggt ctttagaagg aaaaatcgtc acaataggtt
     4921 gtggagataa tttacacgat tgtgaaatat taaaagatga agtaaataaa agaataaaag
     4981 caagaagagt attatttact cgaggaggag tttctatttg ctctcataca gggccagata
     5041 tattggctat aagttgttct aattagtagt aatataaggt ttttgcatat atatgtgaat
     5101 ataattttat attgttaatt tatagtattt ttgataatat agtaaataga gtgatatacg
     5161 tgttttttaa taagaggagg acaagcatta atgtttgaac ataaaatatt taaaatggat
     5221 tttgcaggaa gagaactttc tgtagaaata ggaaagattt gtgaaatggc tagtggtagt
     5281 tgtatagttc gatacagtga tagtatggta atggtaaata caactaaatc agccaaacct
     5341 agagacggaa tagacttttt tcctttaagt gtggattatg aagaaaaatt atactctgta
     5401 ggtaagatac ctggaggttt tttaaagaga gaaggcaagc cttcagagaa agctatactt
     5461 acatcaagat tgatagatag accaataaga cctttattcc caaaaggatt tagaaatgat
     5521 gttcaggtag ttgcaacagt tctatcagta gaccaagatt gtacaccaga tatagtagca
     5581 atgataggtt cttcaatagc cctttctata tcagatatac catttaatgg accaacaggt
     5641 tcagtatgtg ttggccttgt agatggtgca tttgttgtaa atccaaacgc agagcaaaga
     5701 gaaaaaagtt caatgcatct agttgtttca ggtacaaagg aagcaataat gatggttgaa
     5761 gccggagctg atgaagttcc agacgaggta atgcttgatg ctattttatt tgctcaccaa
     5821 gaaataaaaa aaatagttga gtttatagaa ggtatagttg cagaagttgg aaaagaaaaa
     5881 atgcctgttg aattatacca tgcaggagaa gaaataactc aattggttcg cgaatttgca
     5941 actgataaga tgaaaaaagc agttcagact tttgaaaagt tagagagaat ggaaaatatg
     6001 gatagagtta aagaagaaac tttagctcac tttgaagaaa ctttagaaga ttttgaagat
     6061 tttgttggag atatagaaga agtattacaa gatataataa aggaagaagt aagaaaactt
     6121 atagtacatg aaaatgtaag accagataat agaaaacttg aagaaataag acctatatgg
     6181 tgtgaaactg gcatgatacc tagagctcat ggttcagcca tatttactag aggacaaact
     6241 caagtactta atgttgctac tttaggtgct ttaggagatg ttcaaaaact agatgggtta
     6301 gatgaagaag aaaacaagag atatatgcat cattataatt tccctgcata tagtgttgga
     6361 gaagctagac catcaagagg accaggaaga agagaaatag gacatggtgc tttagcagaa
     6421 agagctttat taccagtaat accatctcaa gaagaatttc cttatgctat cagattagtt
     6481 tctgaagtat taagttcaaa tggttctaca tctcaagcaa gtgtatgtgg ctcaacatta
     6541 tctttacttg atgcaggtgt acctataaaa gatatggttg caggtatagc aatgggactt
     6601 ataaagcatg atggtaaggt tgcagtgctt tcagatatac aaggtatgga agaccattta
     6661 ggcgatatgg actttaaagt agctggaaca gagtatggta taacagcaat acaaatggat
     6721 ataaaaattg atggtataga taaagaaatt ctacaacgtg ctttaaaaca agctaaagag
     6781 ggtagaatcc atatactagg agagatgaga aaaactatat ctcagccaaa accagaactt
     6841 tcaccatatg cacctaagat tgttaaaatg caaataaatc ctgacaagat aaaagatgta
     6901 ataggacctg gtggaaagat aataactaag ataatagacg aaactggagt taagatagat
     6961 attgaacaaa ctggggaagt atttatatca ggtattgaga ttgatatgat taagaaagct
     7021 caagaactta taaataatat agtagttgaa ccagaagtag gaaaaactta taaaggtaag
     7081 gtaagtagaa taatgaactt tggagcattt gttgagatac ttccaggaaa agaaggatta
     7141 cttcatatat ctcatatagc tcatgaaaga gttgccaaag ttgaagatgt attgaatata
     7201 ggggatgaag ttgaagttaa agttacagag attgatgaaa aaggaagagt taatttatca
     7261 agaaaagttc ttttaccaaa accagaacat aaaaataaat aacttattat ataattaaaa
     7321 gcaaagtctt aggattttgc ttttttaaat acaaaaaagt agataatttt aaaatgatag
     7381 ttatggatgg cttatagttc tttttctgtc aggctttact cttataaaga agctatttaa
     7441 aagctgatta atagagagct ttattgatat atatagaatc acttaagtta tatttagtgc
     7501 ataaacagag cttattttta atagtattaa ataagaaaca atttgtacat aattaggggg
     7561 agttttaatg ataatgatgg tacttaataa gagtaagctc aaaaaaatca taatactaat
     7621 tttagtagtg ttattagtat tatcaggagg ggtaatggta ttaaagttat ttaacaacaa
     7681 gccagtattt aatttattta atggtgtaga tttaccatat gaaagaggta caaaggataa
     7741 aagtggatat gttgcattaa cctgtaatat agaccttgga tgggaaacag agtacgtaga
     7801 atccatttta gaaacactta aaaaagaaaa tgtaaagatt acatttaatg taactggaaa
     7861 atgggcagaa aagaataagg atgaattatt aaagataaaa aaacaaggac atgaaatagg
     7921 taatcatgga tacaaacatt tagattattc tacattatca tatgaagaca attatgagca
     7981 aatagagacc tctaaaaaga taattgaaga aattataggt gaaaaaacta aattttttca
     8041 agcaccagca ggttcttttg gtccagaaac agtaaaggct gctaaagcat tagggtatac
     8101 ttctatcaaa tgggatgccg atactataga ttggaaatac aaagaccaac cagaggtaat
     8161 cattgataga atgaagaaaa aagacattaa agatagtagt ataattttaa tgcatccaac
     8221 caacgcaacg acaaaatgta tagatgatat tatagctatt gtaagagaaa aaggactaaa
     8281 accaggaaaa cttagtgatg tatttaagta gacaaagtat aaatatttgg ttaagattgt
     8341 ataagttgta gttgaattaa ttaaaagact gacttataat gtaatacata taaaaaataa
     8401 atataattaa aacatagtgt tttcaataat ccaatatgaa acaaagaata aattgttagg
     8461 agaatgtaat gtataagaca aaaatactgg aaaatgggct tacaataatt ggtgaagaaa
     8521 taccatatct aaagtcaata acattaggta tttggataaa tgcaggctct aggatagaag
     8581 aagcacaggt gagtggaact tctcatttta tagagcatat gatgtttaaa ggaaccaaaa
     8641 acagaacatc aaaagagatt gctagttcaa tagataattt gggtggtcaa ataaatgcat
     8701 ttacaagtaa agaatgtaca tgttattatg taaaactaat agatgagcat ataaatacag
     8761 gaattgatgt attgagtgat atgatactaa attctaaatt tgataaaaat gatatagata
     8821 aggaaagact tataatattg gaagaactta agatgtatga agactctcca gatgacttat
     8881 cttatgattt attagtggaa aatatatatg ctaatgatgg attaggaatg aatataattg
     8941 ggacaaaaga atctctatac aatataacta gagaatccat gttagaatac ttaaataagt
     9001 attatattcc aaataacgct gttatttcta tagctggaaa ttttaacttt gatgatatgg
     9061 tggagaaaat caaatcaaaa tttggacact gggaaaagaa aaatttaagt atagacataa
     9121 gtgaggctaa gtttaatcct tgctttatat caaaaaataa agacactgaa caggtgaact
     9181 tagctatgtg cctaaaaggt attccttttg aaaatgatga agaagtctat tcaatggcag
     9241 tggtaaataa tattttcggt ggaagtataa gctcaaggct ttttcagaaa ataagggaag
     9301 aaaaaggttt agtatattca atatactcat cacagacatt atataggaaa tgtggtgaat
     9361 taggtatatt tgcaagcatg agtacagaaa atttacaaga tgtatataat ttaataaaga
     9421 aagaaattga aaatataaga gaaaactatc ttacagaaaa agaaatatct gaaagcaagg
     9481 aacagttaaa aggaaattat atattggatt tagaaagtac tagcagtaga atgatgtcta
     9541 ctggtaaatc tatgttactt agtaaaaaag taaaaactac agatgaaata cttgaatgta
     9601 taaataatgt taatattaat tcaataaaga aggttgttga taaggtattc aatatcgaaa
     9661 atattggtac atgtatagtt ggaagagatg tagaaaagat tctacacttg gattaatatt
     9721 gaaaaaagta aatctctgga ggtataaaat atgaatttat ctgaaatagc aggtaaggaa
     9781 atagttaact tggtcacagg agaaagattg ggggtcattg gagaatgtga ccttatacta
     9841 gatgaaacta caggcggtat attagcgctt ctaattccaa aagaaaaagg attttttgcc
     9901 agaagaaaag ataagtcatt tttagaagta ccatggagaa atgtaaaaaa aataggtaat
     9961 gacatgataa ttatagaata tgagggatat atttaggagg aaaaaatgag tattttagtg
    10021 caaaagtttg gtgggacatc agtagaatca tatgaaaaaa tgaatgaagt atgtaagata
    10081 gtaaaagcat acaaaaaaaa tgatgaagaa ttacaattag tattagtagt ttcagcaatg
    10141 ggaagaaaag gagctccata tgctacagat acacttataa atttgtgtag tgcagtaaat
    10201 gatgagccaa gtaaaagaga acttgattta ataatgtctt gtggagaaat aatatcagga
    10261 acaattcttg ctaatttatt gaatgcacag gggatagatt ctgttttctt tactggtcag
    10321 caagcaggta taattacaag tgatgaatat tctaatgcaa agataaagta tataaatcct
    10381 aaaaaggtaa aaagagcttt agatgatggt aaggttgtag ttatagctgg gttccagggt
    10441 gtaacagatg atggagaaat aactactctt ggaagaggcg gaagcgatac atcagcagtt
    10501 gctattggta aagctttaga atgtgaaaca gttgaaattt acactgatgt ggatggtata
    10561 atgactgcag accctagagt agaaccaaac gctaaagtat tgagttttat agactatgag
    10621 gaagtattcc aaatggctga caaaggtgca aaagtaatac atccaagagc agtacagttg
    10681 gctaaaagtg gaaatataac tttagccata aagaatacaa tgaatccaac ttttgaaggt
    10741 acaaaaatag gttctttatg tagacactta gaagacaata tagaatatga acaagaaaga
    10801 gattttaagg ttgctgttgc aaataaagat agcgtagcac aagttaagat taagtcagca
    10861 gaagaagtgt ttacagaagt attaaatgag atagaaaaga aacttataac tatagatatg
    10921 ataaatttct ttatatcaga aaaagcattt gttgtagaag atgctgatat aaaatcatta
    10981 aaggaaatac tagataaatt tgaattagat tatgaagtaa atagagactg tgctaaggta
    11041 acattgatat gttcaagaat tgctgatgaa atgtctggta tcatgtcaaa ggtagttaga
    11101 ggattatcaa aagcaggggt atctctttta caaacatcag actcaaatat gactatatct
    11161 tgtttagtta gtgaagaaga tatgcataca gcagtacatg ctatacatca acaattttat
    11221 ttaaaataaa ctgtataata caattatttt ttaatcaaat ttaagaattt catatatatc
    11281 ttaatatatt tgatttattt ggtaaaacta aattaaatat aattttgata ggagtttttt
    11341 tatgaaagat tatagagaag ataatgaaag atttgataaa gataattgta gtgaagaatc
    11401 tagttgcgaa gagagagaat gtataaagca atttggtacc aatgaaatgc cacctcaacc
    11461 tcctaaagat attcaatgta taactataat tggagaaatt gagggacatt ttataggaaa
    11521 tcctcaaaaa aaagctacaa agtacgagca tataattcca atgctatact caattgagga
    11581 aagcaatgat gttaaaggag tgttagttgt tttaaacact gttggaggag atattgaagc
    11641 aggtttagct attgcagaac ttttaaacag tacatctaaa aaagtagtta ctcttgttct
    11701 aggaggaagt catagtatag gtgttcctct tgctacagca ggagattact catttatagc
    11761 accaacagca accatgatta tacatccagt gcgaactact ggtcttgtga ttggtataaa
    11821 tgagactttt gagtatttta aaaagatgca agatagaata attcaattta taataagaac
    11881 atcaaatatc aaaaaagatg tacttgaaaa acttatgcat gaaaaagatg aacttgtaag
    11941 tgatgttggt agtgtattaa taggaaaaga agctgtggat tatggtctta tagatgaagt
    12001 tggaggacta aaagaagcac ttaaaaaatt aagagagctt ataaaagaat ctgaggaaga
    12061 aaaaaataat gattaaaatt tgtttaaata ataaaattta tgatataatg atgtaataaa
    12121 gtaggagtgt tagtgttaag gcattagcac tttttgctgt gaaggggtaa aacttgctaa
    12181 aaataaggag tgatattaat ggccacgaaa aagaagaaaa aaaagaaggt aaatacctta
    12241 agttttaatg cagaatatca taatttaata acaatattta taggtgtatt tttattatat
    12301 agtttaaatt ctaattctat gggatggata cctgtgttaa tgcaaaatct ttttaaggga
    12361 ctatttggag ggctttcaat agcaatacca tttatagtaa ttataacagg acttttagga
    12421 ttttttgatg gaaatgaata tatatatagg ctaagaaaga ctaaactata ctacataatt
    12481 attttattta tatttgtatt ttatggatta ttaaatgcag gaactcttcc tgtagatagc
    12541 ccactaaaag gtaacatgtt tgatgatgtt atgaaattgg gtgtaagtgg acaaggtagt
    12601 ggtttgatag caacaactat tgcgtattat atgagtaaaa tatttggtat agcaggtgga
    12661 tggttaataa gtatcttcgc acttatactt tctgtaatgt ttatatttaa tatttctata
    12721 aaagatttgg tgtcaaatgc aaaatcaaaa tctaaagcat ctaaagacag caatttaacg
    12781 tttaaagata aaattgcaaa tatgaaaaaa tctgcaatag atatgatgac tgatgaagta
    12841 gatgatacta ctataaataa aaaaccaggt ttctttaaag ggttaatgtc aaaaggtctt
    12901 aataatgatg atgatgaaga tgaatatttt gaagaagaaa atagtgatgg tgtagatgat
    12961 aaaactataa aaatagttgg atttaataag gcagaagatg agtatttaga aatattagaa
    13021 ggaactcaaa gtatgccaga gttagatgtt ttaaaagaat tacaaaaagc taccaatgaa
    13081 aatccagttg tagatactaa accagaaaaa aaagttgata tagctaaacc taatttaaat
    13141 atagaaaaaa ctcaacctat gagtattgta gcagagccag ttaatgagga ttattcaaac
    13201 tataaaaagc ctagcattga gcttttaaat aaagtaaata agaagtctga tgaaaatggt
    13261 aaaaagaaag tgttgaaaaa tgcttctctt ttagaaaaga cactatcaga ttttggagtg
    13321 gaagcaaaaa ttaatcaagt tacagtcgga cctactataa caagatatga aatacaacca
    13381 agtccaggag tcaaggtaag taaaattgta aatttaactg atgatatagc tttaagttta
    13441 gcagctaaaa gtataagaat tgaagcccca attcctggta aaagtgcaat tggaatagaa
    13501 gttccaaatg aagaagctca aatggttggt gtgagagaag ttctagaaag tgaagaattt
    13561 aataatttta actctcctct tgcaatggga cttggaaaag acgtagcagg aaaaattata
    13621 attggagata taggtaagat gccacactta ctaatagctg gttctacagg ttcaggaaag
    13681 agtgtttgtg taaatacatt aataagtagt attttatata aggcaaatcc tgatgaagta
    13741 aaattactat taatagaccc aaaagtagtt gaacttgcaa attataatgg aataccacat
    13801 ctattgatac cagtagttac tgaccctaaa aaagctgcaa atgctttaaa ttgggcagtt
    13861 acagagatga atagaaggta caaattattt gcagatgctc aagtaaaaga tgtaacaagt
    13921 tataatgaga aagcggaaga aaaacttcct aaaatagtaa taataataga tgagttagca
    13981 gatttaatga tggcaagtgc aaatgatgta gaagattata tttgtagatt agctcaaatg
    14041 gcaagagctg ctggaatgca cttgattgta gcaactcaaa gaccatctgt tgacgttata
    14101 acaggggtaa taaaggctaa tataccatct agaatagcat ttgcagtatc atctcaaact
    14161 gattcaagaa caatccttga tatgggtggt gcagagaagc ttctaggtaa aggtgatatg
    14221 ttattctatc cacttggtgc agcaaaacca gttagacttc aaggggcatt catatcagag
    14281 agtgagtcgg aaaaagtaat cgattttgta aaaagtcagg ttaaagatgg aataaaatat
    14341 gaagaagata taattgaaac tatatctaag gttaatactt caaagggtag tgatgaggat
    14401 gagtttttga gtgaagcgat agaatttgtc gtggaaagtg gtcaagcatc tgcctctatg
    14461 ctacagagaa gatttaaaat aggattcaat agagcagcta gacttataga ttctatggaa
    14521 gaaagaggaa taattggagc aagtgaggga agtaaaccga gaaaggtatt aataagcaaa
    14581 caggatttgc agaatttaga aggtgaatag aattgtcagt tataaaaata gaaaaagcac
    14641 ttaagctaga taaagtaata tttgtagatg tgagaactga aggagaatat gaagaagacc
    14701 atatattgaa tgcaatcaat atgcctcttt ttaaaaataa tgaacataat gaagttggaa
    14761 caatttataa gatgcaggga aaacatgaag caatacaaaa agggtttgac tatgtatcgt
    14821 ataaattaaa ggatatatat ttacaagcag cagaacttgc attaaattat gataatattg
    14881 tcatatattg tgcaagaggc ggaatgagaa gtggcagtat agtaaattta ttatcatcat
    14941 taggtgtaaa tgtatatcaa cttgaaggtg gatataaggc ttatagaaat tttgttttag
    15001 agtatttaag gcatgtaatg gatacaaaaa actttgtatc tgttcatgga ttgactggta
    15061 caggaaagac tgatttgtta catttattag atgaaaaagg aatagacgta ctggatttag
    15121 aaggcatagc taaaaacagt ggttctacct ttggttttat aacttttgat aaaaagccac
    15181 catctcaaaa gaactttgaa acaaaactat ttgaaagtat atttttttcg aaagagaatt
    15241 atatatttgt cgagagtgaa agcaaacgag ttggaagtgt tacagttcca aatgaaattt
    15301 tcgaggcaat gacacgagaa ggacatcata tattattaga atgtgatatt gaaaatagag
    15361 ttgatagact ttgtagagat tatatttatg ataaagatga aggtaatata ttgattttaa
    15421 aagaatgtat aaacaagttt agaaaaagat tgggtaataa agtagtagat gattatattg
    15481 atttattaga atcaggtgaa tataaagaac ttgtaaaaag atatctttta gaatactacg
    15541 acccacttta tatgcattca gtagagaaat ataagtatag taagattata aattttaatg
    15601 atacacaaaa agctttagaa gatgttatag acttatatga gagtatattg gaaggagaaa
    15661 gtgaatgtta aaaatagcat tggaatcatt aggatgctca aaaaatttag tagatgcaga
    15721 aataatgatg ggaattttaa ataacaaagg ctataaatta attggagatt ttgaagaagc
    15781 tgatgtaatt atagtaaata catgtgggtt tatagagtca gctaaacaag aatctataga
    15841 tacgataata aactttgcag agttaaaaaa aactggcaat ttaaagttgt tgatagtaac
    15901 agggtgtcta gcccaaagat attctgaaga attaaaaact gaaattcctg aaatagatgc
    15961 tatagtagga acaggaagct atcaaaatat agataaaata cttaaagaat taagtgaaat
    16021 gcatcaaatt gtaagcctta atgatataga gtttgtattt aatgaagatt taccaagata
    16081 tatatctaca cctagctata tggcctattt aaaaattgga gaaggatgct ccaataattg
    16141 tacttattgt ataataccaa aactaagagg taaatataga agtagaaaat ttgaagatat
    16201 aattaaagaa gctaagaaat tagctgaatc tggagtaaaa gaacttgtgg ttatagcaca
    16261 ggatacgaca aagtatggtt ttgatttata tggaaaagaa agattatcag aacttttaga
    16321 agaacttgca aaaatagatg gatttaagtg gataagggtt atgtattcat atccagaatc
    16381 tataacagaa gaattgatac aagttataaa gaaatatgat aatatatgta gttactttga
    16441 tatgccaata cagcatgcaa gtaacaatat tttaaaatta atgaatagaa aaactactaa
    16501 agaagatata ttaaataaaa tcaatctgat aagaagtaat ataccagatg ctatacttag
    16561 aactacaata atagtagggt ttcctggtga aacagaagat gacttcaaac aacttgtgga
    16621 ctttgttgaa gaggttaaat ttgatagact tggtgcattt gcatattcaa gagaagaaga
    16681 tacaccagca gatagattac caaaccatat agatgaagaa gtaaaaatac aaagaagaga
    16741 tactttaatg atgattcaac aaaaaatatc tgaagaactt aatgacaaaa agataggaaa
    16801 aacttatgag gtgttaatag aagaacagat agaagataat gtatatacag gaagaacaca
    16861 aggtgatgca gaagaaattg acagtatagt gtatgtaaaa agtgtagaca atcttgaagt
    16921 aggagaattt gtaagtgttc aaataaatga tgcaatggaa tacgatttaa tgggagatgt
    16981 tttatatgaa cttgcctaac aagttaactt tgtttagaat atttttaata ccagtatttg
    17041 ttttaattat gttgctcaat gtaccaaata aatttttaat agcttgtatt atttttataa
    17101 tagcttcaat tactgatgct ttagatggaa aaatagctag aaaatacaat ctagtcactg
    17161 actttggaaa atttatggac ccattagcag ataagttatt ggttatttca gcacttacat
    17221 gtatgataga agaccatctt gtaagtagtt ggatggtaat tataatagtg gctagagagc
    17281 ttacagttag catattaaga gctatagctg ctgctgatgg aaaagtaatt gctgctggaa
    17341 acagtggaaa acttaaaacg ataactcaaa tggtatctat agtgttttta ttgttaggtg
    17401 ctcagtttga aaatgtattg atattaaata ttggtgaaat tctcattctt atagctacac
    17461 tacttacatt atattctgga tgggaatatc tatataaaaa taaagagctt tttatgtctt
    17521 ctaagtaaaa aattatgctt taataaataa aaactaatga ttaataaata aaaaataaca
    17581 acttaaaatt ttaaaaatgt aaaaaagggt atactattat agtatacctc ttttcatttt
    17641 aagaatattt tcttttgtgt atataattat taataatggt ttataaataa aatgtaatga
    17701 ataaaaatat aatcattagt agcgttttta aattgactag gtttgattta tgttatataa
    17761 ttaatatata acaagagaac aaatgtttgt agataaatga agggatggta tagagatgag
    17821 tgtagatcaa gaaaaattaa aagcgttgaa tgaagcttta ggtaaaattg aaaaagattt
    17881 tggtaaaggt tcagtaatga aattgggaga agcaacatct atgtctatag atgttatatc
    17941 aacaggagcg attggtttag acatagctat tggtatagga ggtctaccta gagggagaat
    18001 agttgaagta tatggtccag aatcttctgg taagactact gttgcgctta gttgtgtagc
    18061 atcagctcaa aaagatggag gaatagctgc atttatagat gcagaacatg cacttgaccc
    18121 agtatatgca aaagctttgg gtgtggatgt tgataaccta ataatatctc aaccagatac
    18181 aggtgaacag gctttagaga tagcagaggc attgataaga agtggagcga tagatataat
    18241 agtaatagac tcagtagcag cattagttcc aaaggctgaa atagatggag atatgggtga
    18301 ttctcatgta ggtttacaag ctagacttat gtcacaagca cttagaaagt taactggttc
    18361 aattaaaaaa tcaaattgtg ttgctatatt tataaaccag ttaagagaga aagtaggaat
    18421 aatgtttgga aacccagaaa ctactactgg aggacgtgca ctaaaattct attcatcagt
    18481 tagattggat gttagaaaaa tagatacaat aaaacaaggt gataaagtta taggtagtag
    18541 aactagagtt aaagttgtta aaaacaaagt agcaccacca tttaagcaag ctgaatttga
    18601 tataatgtat ggagaaggga tttcaaaaat tggagacctt ttagatatag ctgctgatgt
    18661 agatatagtt aaaaaatcag gttcatggta tagttacaat gatactaaac ttggacaagg
    18721 aagagaaaat gttaaaaaat tcttggaaga taatttagat ttaactactg aaatagatga
    18781 gaaagttaga gcattttaca atttaaatga agaacatgaa gaatcaggta cttcagtatc
    18841 aaaagaaatt gtagaagaat aagtaaaaat acaaaaaaat aactagtaat taaagacatg
    18901 ccacgctccc tattagcttg acacaatgtt gaaaaaatta taaaattaag tgaggataaa
    18961 atatctaaat ttaattttaa ttattaggaa tattttttaa tcatagtgat gacattttgg
    19021 gattattaac gaaggaggtg gatgtcatag atagtgtagt aatgattgta ataggtgcag
    19081 ctgttggtat tatagctgga tattttgtta gaaaaaatat atctgaagca aaaattggac
    19141 aagctgaaaa cttggccaaa gaaataatag ataaagcaca ccacgactca gaaacagtac
    19201 aaaaagaaaa attactagaa gctaaagaag agatccataa gtggagaaca gaagctgaaa
    19261 gagaaaacag agaaagaaga acagaagtac aaaagtatga aaaaagagta gtacaaaaag
    19321 aagaagtctt agatagaaaa ttacaaaatt tagaatctaa agaagtaaac ctaagtgaaa
    19381 aattaaaggt agtggaaaag aaagaagaag aagttgaagt aataaaaact caacagttag
    19441 aaaaattaga aagtatatca ggaattactt ctgataaggc caaagaaatc atcttaacca
    19501 atgcggagag agatgtaaga cgtgaaatgt ctataatgat aaaagagata gaaagtcaag
    19561 ctaaagaaga agctgataag aaatcaagag aaattatagg atatgcaatt caaaaatgtg
    19621 cagcagatca cgtagccgaa actactgtaa cagtagtaag cttgcctaat gatgaaatga
    19681 aaggtagaat aataggtagg gaaggtagaa atataaggac tttagaaacc ttaacaggta
    19741 tagaccttat aatagatgac actccagaag ccgtaatttt atcaggattt gatccaataa
    19801 gaagagaaat tgcaagaatt gctctagaaa aattaatagc agatggaaga atacatccag
    19861 ctagaattga agaaatggtt gaaaaggcga gaaaagaagt tgacaatatt ataaaagaat
    19921 atggtgaaca agctgcattt gaaactagtg tacatgggat tcatccggaa ttaataagat
    19981 tattaggtag acttaactat agaactagtt atggacaaaa tgtacttaaa cattctatag
    20041 aagttgctca tatagctggt ataatggctg ctgaaatagg agcagatatt agattggcta
    20101 agagggcagg tttattacat gatataggta aggctgtaga ccatgaaatg gaaggtacac
    20161 atgttgaaat aggtatggat ttacttagac gttataaaga gtctaaagaa gttatacatg
    20221 ccatgagtac tcatcatgga gattacgagc ctcaaacagt agaggctgta ttagttacag
    20281 cagcagatgc aatatcagct gctagacctg gtgcgagaag agaaacttta gaagcatata
    20341 taaagagact tgaaaaatta gaagaaatag ctaattcata tgaaggtgta gaaaaatcat
    20401 ttgctataca agctggtaga gaaataagaa taatggtaaa accagaagct ataaatgatg
    20461 aagaaataca tctattggct agagatatga ctaagaaaat agaggatgaa cttgaatatc
    20521 ctggacaaat aaaagtgagc ataataagag aaacaagagc tatagaatat gcaaagtaaa
    20581 agaaataata tctaaataat aagaaaatat ttttagaaat ttaaatttgc cacaagatat
    20641 gataatatat cttgtggctt ttaaattgtt taagtttaat tttatataat gcaaaaataa
    20701 tctaagtgct aaaagtgcta cttacaaaag attaataaaa taaagattta tatatggatt
    20761 atttatataa ttatccaagt ttgtttgata taatgaagtt gtaaaatcaa tactttacta
    20821 caaaataaat agtaaatact acagtaaaat ctaaaaatag gatataatag tattaatata
    20881 agaagattat aaataaaacc tgctaatgaa aatagattat
//
LOCUS       DAKBNN010000051        20800 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000051 DAKBNN010000000
VERSION     DAKBNN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.051"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..321
                     /locus_tag="OUB02_001622"
     CDS             <1..321
                     /locus_tag="OUB02_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCT8885384.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="OUB02_001623"
     CDS             337..1920
                     /locus_tag="OUB02_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HCT8885385.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="OUB02_001624"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="OUB02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCT8885386.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEIAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIISGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="OUB02_001625"
     CDS             3475..4827
                     /locus_tag="OUB02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCT8885387.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="OUB02_001626"
     CDS             5019..6128
                     /locus_tag="OUB02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8885388.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="OUB02_001627"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="OUB02_001627"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HCT8885389.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQDKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="OUB02_001628"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="OUB02_001628"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HCT8885390.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLVGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="OUB02_001629"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="OUB02_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCT8885391.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12653..13675
                     /locus_tag="OUB02_001630"
     CDS             12653..13675
                     /locus_tag="OUB02_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT8885392.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13735..14742
                     /gene="gap"
                     /locus_tag="OUB02_001631"
     CDS             13735..14742
                     /gene="gap"
                     /locus_tag="OUB02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCT8885393.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14964..16166
                     /locus_tag="OUB02_001632"
     CDS             14964..16166
                     /locus_tag="OUB02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCT8885394.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16192..16935
                     /locus_tag="OUB02_001633"
     CDS             16192..16935
                     /locus_tag="OUB02_001633"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCT8885395.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16944..18476
                     /locus_tag="OUB02_001634"
     CDS             16944..18476
                     /locus_tag="OUB02_001634"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCT8885396.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQVSMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            19064..20356
                     /gene="eno"
                     /locus_tag="OUB02_001635"
     CDS             19064..20356
                     /gene="eno"
                     /locus_tag="OUB02_001635"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCT8885397.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         8320 a         2366 c         4154 g         5960 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttatga gaactttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatag ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aagtggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggattaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagataa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat ccagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaatct
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagttggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcagca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaaaggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggctca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta ggacacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagag gatggagtaa taatctattc
     8881 aacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacaggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagtgca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagtcaac tctgttccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagct
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatataaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaacataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataataga atatggaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaag taactagaaa taaaattatt tatagtaata
    12421 aatattagaa tgaaaatata aaaaaatata tataaaaagc cttatataaa aagaaaaaat
    12481 actgtgattt gaaaatggtg gagaaatatt ttataaaata aaaaaataaa aaagtttttg
    12541 aaaaactatt gaaatctata cgcaaatcct ataaaataaa aatatcttgt gggacttaaa
    12601 taaacactag ggacaaaaaa agtcccgaaa ggaaagcaat aaggagtatg ttatgaagaa
    12661 cttattagaa attcaaaaga aattgattcc acaggcaatt gaacttatgg aaagaagata
    12721 ctctatttta agacaaatat ctttaagtga accaataggt aggagaacat tatcaaatgt
    12781 attagagatt agtgaaagaa taatcaggtc agaaactgag tttttaaaag aacaaggact
    12841 tatagacgta gctgtatctg ggatgactat cactaaagaa ggttctgatt tgttagataa
    12901 acttaaggac atcatgagtg acatcatggg attgtctaag ttacaagata gagtcagaga
    12961 aaagcttgga ataaaaaggg taatattagt tcctggaagc tgtgatgaaa atggaagttt
    13021 actaaaagat gtaactagat atggatgcga atatttctta ggcattttaa aagatggaga
    13081 tatcgtttca ataacaggag gaagcactat gttggagttt tcaaacgtta ttaaaactga
    13141 taagagatat ccaaactcta caatagttcc agctagagga agtatgggaa ctgatgtaga
    13201 gatgcaatct aacaatatag ttgcttcaat aagtaagaaa ttacattcta attgcaaact
    13261 tttacatata ccagatgagt tggatgaaag tgcaatgaaa actcttagcc aagtgccaga
    13321 gattaagaca actttggagt atattaaaag aacagacatt ttagttttta gtataggtag
    13381 agcagatgtc atggcaaaga gaagaaaact tccagaagac aaggttgatg agatattatc
    13441 taaaaatgca gttggagaag catttggata ttatttcaac aaagatggag aaatagttta
    13501 caaactgaac actgttggaa ttgatttaga aacagttaaa caagtaaaag aaactattgc
    13561 aatctttgca gggactgaaa aagtagaagc cttattagct atatcaaata tgcataaaaa
    13621 catggttctt gtaacagatg aagaaagtgc ttataagata ttgtcattat tataaaagtc
    13681 aagacctagc acagagctag aaaacataca tttactttat aggaggtagc gaaaatggtt
    13741 aaagtagcta taaatggatt tggaagaata ggaagattag cattaagaaa aatgatggag
    13801 caacaagata agttcgaagt tgttgcaata aacgacttaa cagacgctaa aatgttagct
    13861 catttattta aatatgatac agctcaagga agatttaacg gagaaataga agttaaagaa
    13921 ggtgcttttg tagtaaacgg aaaagaaata aaagtaacag cagaaagaaa tcctgctgac
    13981 ctaccatggg ctgaattagg agtagatata gtattagaat gtacaggatt ctttacatct
    14041 aaagataaag ctgaagctca tatacaagca ggggctaaga aagttgttat atctgcacca
    14101 gctactggag atttaaaaac aatagtattc aacacaaaca gtgacatatt agatggttct
    14161 gaaacagtta tatcaggagc ttcttgtact actaactgtt tagctccaat ggctaaagta
    14221 ttaaatgata aatacggtat agaaaaagga ttaatgacta ctatacatgc atacactaat
    14281 gaccaaaata ctttagatgg tcctcatcca aaaggtgatt taagaagagc tagagctgct
    14341 gctggaaaca tagttccaaa cactacagga gctgcaaaag caataggctt agtaatacct
    14401 tcattaaaag gaaaattaga tggggcagca caaagagtac cagttgtaac tggttcaata
    14461 actgaattag tttgtacatt aggaaaaaat gttacagttg aagaaataaa tgcagctatg
    14521 aaagaagctt caaacgaatc tttcggatat actgaagaaa tgttagtatc ttctgacata
    14581 atcggaataa gctacggttc attatttgac gctactcaaa caaaagttat ggaagtagat
    14641 ggaaaacaat tagttaaagt tgtttcttgg tatgataatg aaatgtctta tacttctcaa
    14701 ttaataagaa ctttaggata ctttgcacaa ttagcaaaat agtcagtttt gagaaaatag
    14761 aaaagtagat tgacaaagtc cgagtttgta gtttaatact acggactcag gacttttttt
    14821 aagaaaaaat atataaaata cacctaaata atgttacata ctatatataa aaatatgata
    14881 atatgatata tttaataggg taaaagtatg tactatatta tacttggcaa taaaaaatta
    14941 ctttattaaa aggagagata tttatgtcaa tgcttaacaa aaaaactata gaagatattg
    15001 atgtgtgcgg aaaaaaagta ttagtaagat gtgactttaa tgtgccatta caagatggtg
    15061 taattactga tgaaaataga ttgaacggag ctttaccaac tattcaatat ttaatttcta
    15121 aaggggcaaa agttatatta tgctctcact taggaaagcc aaaaggagaa gctaaaccag
    15181 aattatcttt agcaccagta gcaaagagat tatctgagat gttaggaaaa gaagttgtat
    15241 ttgcagcaga cgataatgtt gtaggagaaa atgcaaagaa agcaactgaa aaaatggaaa
    15301 acggagatgt agtattatta gaaaatacta gatatagaaa agaagaaact aaaaacgaag
    15361 agaatttctc taaagaacta gcatcacttg ctgaaatatt tgtaaatgat gcatttggaa
    15421 ctgctcatag agcacattgc tcaacagttg gtgcaggcga gtttttacaa gagagagttt
    15481 gtggatactt aattcaaaaa gaattaaaat tcttaggaga agctgttgca aaccctgtaa
    15541 gaccatttac agctatactt ggaggagcta aagtttctga taaattagct gttataaacg
    15601 aattattaga aaaagttgat aatctaataa taggtggagg aatggcttat acattcttaa
    15661 aagctcaggg ttatgaagta ggaacttctt tacttgaaat agataaagtt gaatatgcta
    15721 aagaaatgat ggaaaaagct aagaataagg gtgttaactt attattacca gtagacgttg
    15781 ttatggcaga ccactttgca ccagatgcta ctccaatagt aacagaagat gctaatgtaa
    15841 aagaagatta catgggacta gatatgggac ctaagactat agcaaacttt gtaaaaacta
    15901 taaaagaatc aaaaactgta gtatggaatg ggccaatggg agtatttgaa tttgaaaact
    15961 ttgcaaatgg tacattatca gtagctagag ctatggcaga gttgactgat gcaactactg
    16021 taattggcgg tggagatagt gcagcagcag ttaatcaatt aggatttggt gacaagatga
    16081 ctcacgtatc aacaggtgga ggagcatcat tagaattttt agaaggtaaa gaattaccag
    16141 gtattgcagc attagataat aaataaacta atatatagag gagattgtaa aatgagaaaa
    16201 cctataattg caggaaactg gaaaatgcat aaaacaataa aagaagcttt agaatttgta
    16261 aatgaagtta aagataaagt aaattctgat aaagtagaag ctgttatatg tgcaccattt
    16321 actttattaa aggatttaaa agaagctact aagggtacaa atataaagat aggtgctcaa
    16381 aatatgcact ttgaagaaaa aggagcattt acaggagaag tttcacctct tatgttaaaa
    16441 gaaatagata tggactatgt tgtaatagga cattcagaaa gaagacaata cttcaatgaa
    16501 actgatgaaa ctgtaaacaa gaaagtttta aaagcattag aagtaggaat agacccaata
    16561 ttatgtgttg gagaaacttt agaacaaaga gaagctggaa aaactaaaga tgtttgtaaa
    16621 gttcaagttg aaaaagcttt agaaaatgtt cttaaagatg atttagcaaa agtagttgtt
    16681 gcatatgagc caatctgggc tattggaact ggtaaaactg caactgctga agatgctaat
    16741 gacgttatat cttatataag agaagttata aaaggattat atggagaatt agctaatgaa
    16801 gttagaatac aatacggtgg aagtgttaaa ccttcaaatg tagccgaaat aatgggtcaa
    16861 agtgatatag atggagcttt agtaggtgga gctagtttag ctagtaatga ttacctagac
    16921 cttgttaatt tctaaggagt gaaatgatga aaaaaccagt tgctttaatt attatggacg
    16981 ggtttggata caataaggat gttaagggaa atgcaattgc tgagtccaaa actcctaatt
    17041 tagatagaat aaaaaaagag tatccaaaca cattgataaa tgctagtggt cttgatgttg
    17101 gtctacctga tggtcaaatg ggaaattcag aagttggaca cactaacata ggtgcaggta
    17161 gaatagtata tcaagattta actagaataa caaaatctat aaaagatggt gactttttta
    17221 ctaataaagt tttatgtgaa gcaatggata atgcaaaaga aaactctctt catgtaatgg
    17281 gacttttgtc tgatggagga gtacattcac atatagacca tcttaaggct ataatcaaaa
    17341 tggcaaaaga taaaggcgta caaaaggtgt atgttcacgc atttactgat ggtagagata
    17401 ctgaccctca aagtgcatta gaatatgcca aagaagtaca agttagtatg gatgaaattg
    17461 gagtaggtga atttgctaca gtttctggta gatactacgc tatggataga gataagagat
    17521 gggaaagagt agaacttgca tacaatgcca tggttagagg tattggtgaa aaagcaaatt
    17581 ctattgagga agctattcaa aactcttatg atgatggaaa aaatgatgag tttataatgc
    17641 caacagttat tatgaaagat gataaaccag ttggaagtat aaaggaaaat gattcaatta
    17701 tattcttcaa ctttagacca gatagagcta gacaaataac tagagcttta gtttgtgaag
    17761 agtttgatgg atttaaaaga gaagatatta aaaacttctt tgtatgctta acagagtacg
    17821 atataactat agagaatgtg catatagcat ttggacctca atctttagcc aacactctag
    17881 gtgagtatct agctaaaaat ggaaaaactc aacttagagc tgctgaaact gaaaagtatg
    17941 cacatgtaac tttcttcttc aatggtggag tagaagagcc aaataaaggt gaagaaagac
    18001 tattgatacc ttctccaaag gttgcaactt atgatttaaa accagaaatg tcagcatatg
    18061 aattgacaga taaagcttta gataaactag gagaagataa gtttgacttt atagtattaa
    18121 actttgcaaa tcctgatatg gtaggtcata caggaagtat agaagcggct ataaaggctg
    18181 ttgaaactgt agatacttgt gtaggaaaat tgatagataa aatagtagag ttaggtggaa
    18241 gtgcaataat aactgctgac cacggaaatg ctgaatatat gttagaccca gaaacaggaa
    18301 aaactgtaac ggctcactct attaatccag taccatttat tgtagttggt caagaatatg
    18361 aaagtgcaaa attattggat ggaggaagat tatctgatat agcaccgact gttttagata
    18421 tgatgaagtt agaaaagcca gaagaaatga ctggtcattc acttatttca aaatagtcga
    18481 ttttgataag tagagaataa tatataaact acaatataaa tttaaaaata atgcagttta
    18541 ggtttatttt gtaaagttta gactttatga cataagattt aaattttgta agttctggat
    18601 attgtaaggt gtagatgaag atatgtttta taaaatttac aagatagaca atttataaaa
    18661 taaaaaatta caatatagat aataatgtaa ataatgatat aaataataac aagatagaca
    18721 ataacaatat aaataataat aattaatata tattatataa gcaaaaattt tagaatcatt
    18781 agttgtacat tttatgttta atgttttaaa tctagttata atatttgtgt atttaagatt
    18841 ggttttggat tttagacaag tttttttata ttatataaat taaaatatta taaaaaataa
    18901 aataaaaata ggaactttta caaatttacc catcaaataa atttgtacct aattttcaaa
    18961 agcaacttta tgtactataa tatagaaagt agattaaaat agtagacgga tgtttacagt
    19021 aaatgaaaat ttaactaata gaaaatcgaa gggagatata caaatgtcag ttattgaatt
    19081 agtatatgct agagaagtat tagactcaag aggaaatcca actgtagaag ttgaagtagt
    19141 attagaagat ggagcaatgg gaagagcaat agttccatca ggagcatcta caggagcttt
    19201 tgaagctgtt gaattaagag atggagataa gggaagatac ttaggaaaag gtgtagaaac
    19261 agctgttgct aacgtaaatg aaataatagc acctgaaata gaaggaatgg atgcatttga
    19321 ccaaccagct atagatgcaa tcatgataga attagatgga actccaaaca aaggtaaatt
    19381 aggagctaat gctatattag gtgtttcaat ggcagtagct agagcagctg ctgatgaaat
    19441 aggtttacca ttattccaat acttaggtgg agtaaatgct aaacaattac ctgttccaat
    19501 gatgaacata ttaaacggtg gagagcatgc tgataacaac gttgatgttc aagaatttat
    19561 gatattacca gtaggagctt gttgtttcaa agaaggttta agaatgggag cagaagtatt
    19621 ccattcatta aagaaagttt taggagaaaa aggattagct tgtggtgtag gtgacgaagg
    19681 tggatttgct ccaaacttag gttcaaacag agaagcttta gaattaatag ttgaagctat
    19741 aacaaaagct ggatataagc caggtgaaga cgttatgtta ggtcttgacg ttgctgctac
    19801 agaaatgtac aacaaagaaa ctaaaaaata cgttttagca ggagaaggaa aagagttaac
    19861 ggctgctgaa atggttgctt tatatgaaga ttggtcaaac aacttcccaa taataactat
    19921 agaagatggt ttagacgaag aagattggga tggatggaaa ttattaactg aaaaattagg
    19981 aaataaatta caattagttg gtgacgactt atttgttact aacacagaga gattagaaaa
    20041 aggaattgaa aatggagtag ctaactctat attagttaaa gttaatcaaa taggtacaat
    20101 aactgaaact ttagatgcta tagaaatggc taagagagca ggatatactg ctgttatatc
    20161 tcacagatca ggagaaactg aagattctac tatagcagat ttagctgttg cagtaaatgc
    20221 tggtcaaata aaaactggtg ctccatcaag aactgataga gttgctaagt acaaccaatt
    20281 attaagaata gaagaaatgg ttggagagca agctagatat tgtggattaa aatctttcta
    20341 taacttaaaa aaatagttat taaaatggtt actaaaaaag attaatgaag agagaatata
    20401 agatatttaa gctcatctta taagatataa aagagataag tgttaattga tttttcaatt
    20461 cacttatcct tttttatatt ttattactta attttattca tagattcaga aaaaatatga
    20521 ataaatgttt ctatctcatt tttatctaat tgcataggag gtttttatta aaaaataaaa
    20581 gtgaagacat attttgattt aatcataatt gtcattaaag tattattgct aaatgtggat
    20641 tatttatttt aaattatgtt gaaattaaaa gttatatcaa tattttaaaa tgattgtttt
    20701 attttggtgg ctatttataa tatatgcaat gagagtagat tattttaagt gttaatttat
    20761 gcattaattt atgtattaat ttaaatagtt ttctcttcta
//
LOCUS       DAKBNN010000052        20332 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000052 DAKBNN010000000
VERSION     DAKBNN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.052"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            142..879
                     /gene="cas6"
                     /locus_tag="OUB02_001636"
     CDS             142..879
                     /gene="cas6"
                     /locus_tag="OUB02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HCT8885398.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESVRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            884..2359
                     /locus_tag="OUB02_001637"
     CDS             884..2359
                     /locus_tag="OUB02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885399.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIVKNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNGNLSNLKELLLDNISFLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2379..3392
                     /locus_tag="OUB02_001638"
     CDS             2379..3392
                     /locus_tag="OUB02_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HCT8885400.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3408..4205
                     /gene="cas5"
                     /locus_tag="OUB02_001639"
     CDS             3408..4205
                     /gene="cas5"
                     /locus_tag="OUB02_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HCT8885401.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVALKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4232..6640
                     /locus_tag="OUB02_001640"
     CDS             4232..6640
                     /locus_tag="OUB02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCT8885402.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKNILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQKEIYDYIE
                     KGILEKEDFNLEDIEDYIKEILYVDFKFNEDILDYDYIDKLSESMVIYSDYNNGQIED
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7082..8029
                     /gene="prmA"
                     /locus_tag="OUB02_001641"
     CDS             7082..8029
                     /gene="prmA"
                     /locus_tag="OUB02_001641"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCT8885403.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGIAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8048..8806
                     /locus_tag="OUB02_001642"
     CDS             8048..8806
                     /locus_tag="OUB02_001642"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCT8885404.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8808..10106
                     /gene="mtaB"
                     /locus_tag="OUB02_001643"
     CDS             8808..10106
                     /gene="mtaB"
                     /locus_tag="OUB02_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCT8885405.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10149..10499
                     /locus_tag="OUB02_001644"
     CDS             10149..10499
                     /locus_tag="OUB02_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HCT8885406.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10662..10841
                     /locus_tag="OUB02_001645"
     CDS             10662..10841
                     /locus_tag="OUB02_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCT8885407.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10871..11314
                     /locus_tag="OUB02_001646"
     CDS             10871..11314
                     /locus_tag="OUB02_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCT8885408.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11425..11922
                     /locus_tag="OUB02_001647"
     CDS             11425..11922
                     /locus_tag="OUB02_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCT8885409.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12115..12678
                     /gene="raiA"
                     /locus_tag="OUB02_001648"
     CDS             12115..12678
                     /gene="raiA"
                     /locus_tag="OUB02_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCT8885410.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12891..13127
                     /locus_tag="OUB02_001649"
     CDS             12891..13127
                     /locus_tag="OUB02_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HCT8885411.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13140..14483
                     /gene="yqfD"
                     /locus_tag="OUB02_001650"
     CDS             13140..14483
                     /gene="yqfD"
                     /locus_tag="OUB02_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HCT8885412.1"
                     /translation="MISFIRGYYVIVVEGVRLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14422..15537
                     /locus_tag="OUB02_001651"
     CDS             14422..15537
                     /locus_tag="OUB02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCT8885413.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15594..16055
                     /gene="ybeY"
                     /locus_tag="OUB02_001652"
     CDS             15594..16055
                     /gene="ybeY"
                     /locus_tag="OUB02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCT8885414.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16061..16780
                     /locus_tag="OUB02_001653"
     CDS             16061..16780
                     /locus_tag="OUB02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCT8885415.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16899..17300
                     /locus_tag="OUB02_001654"
     CDS             16899..17300
                     /locus_tag="OUB02_001654"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCT8885416.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17400..18293
                     /gene="era"
                     /locus_tag="OUB02_001655"
     CDS             17400..18293
                     /gene="era"
                     /locus_tag="OUB02_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCT8885417.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18305..19684
                     /gene="mgtE"
                     /locus_tag="OUB02_001656"
     CDS             18305..19684
                     /gene="mgtE"
                     /locus_tag="OUB02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCT8885418.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19757..19912
                     /locus_tag="OUB02_001657"
     CDS             19757..19912
                     /locus_tag="OUB02_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HCT8885419.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19878..>20332
                     /gene="recO"
                     /locus_tag="OUB02_001658"
     CDS             19878..>20332
                     /gene="recO"
                     /locus_tag="OUB02_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCT8885420.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIV"
BASE COUNT         8498 a         1892 c         3710 g         6232 t
ORIGIN      
        1 aataaaacta acagtaaaat atatttaaaa aattaaaaaa caaaattaaa tgtattatca
       61 aaaaatcatt ttatgatatg ttttattttt atattacaaa tagagaataa tgaattatac
      121 tagaaagtag gaggtgaaaa tatgagcaga atttcaattt ttatggaatc atgtaatggc
      181 aaatttccaa atgaaaattc aatgttgtcg gtttccttta taaaaaatat tttaaatgca
      241 gaaaataaag attttgctaa aaatatattt aactatgggg aagaaagaaa aaacaataag
      301 caaataaagg atataaatac agccatttac ataccaaatc ttataagaag caaaaatgag
      361 ttacttgttg atggagatat aatattcaat ataagttttt ataattattc tatgttcagt
      421 aaattataca atggaatttt aaaagtaaag gagctagaat ataaaggtta tagatttaaa
      481 ataaaaaata taaaaataca taaagaacac gagataaaag aaaatggtgt tatatttaaa
      541 actatgtctc ctataatagt aaagaataaa gaaggtaaat atttagatgt agaagattca
      601 aattatatag aatgtttaaa ctatatagct aatctaactc tggagagtgt aagaggtagt
      661 gggcttagaa agccattgga atttatacct cttaacttca aaaaaagagt gttaaaagaa
      721 aagattcgtg gatttaaagt gagagaattt tactatataa atgcttatgc aggaacattt
      781 tttttaaaag gagatatgga agatttaaat gcactgtata aaatgggaat tggatataga
      841 aggacggaaa atgcaggaat ggttgatata ttaaagtagc gaggtgagag aaataaaagc
      901 taaattttat agatcaggtg atattgtaag agatattggc attgtttact tttatgaatt
      961 actaatggat ttaaaaaacg aactagaaaa aaatgattat aagctaaaat ttacttgtga
     1021 gctaaataga aattatttat ctttagaatc tgcagaatat atagacccta agtatatatc
     1081 tgattatata ttagaaaatc aactttttaa ggtatttaaa aatgggaaaa aggaaacctt
     1141 agaaaaaata tttagtaata tagataattt aacgtatgta aattttattg aagaattgga
     1201 taaatcttct gcaactgcta aacaaaagga agatattaaa aaagatttta aaaacaggag
     1261 gtttccatat gttagaaata gtggtaagta tggactaaac acgagtttag aaaatatgga
     1321 aacaaatgta aaaagaatag tagatactgt aattaaatca aatatagaga tagatgaaat
     1381 agataaactt gatttgacac aatatgaaga aaaagatagt gtatgtaatg tttgtaatat
     1441 aaataaaaca acaaaactag atattgattt aagagagcga aaggactcta agtataactt
     1501 tttatttaga ggtgcagaaa aatcaggctt taaaagaagt ggacaagttg aaagtaatat
     1561 ttgttttgag tgtgaatttt tcaacttaat gtgtctttta tatataaatt taaaaagacc
     1621 aatggttttt gcatatacga atgatttaag agaattagca tttttaaatc ataagataat
     1681 gcttaaaaga aaaatgtatt cagataaatc attttataaa aaacttttgc atgaaaaaat
     1741 aagttcaatt agactatata gatttgatat tgttaagaat aaaggtataa ttctaaagtt
     1801 tgactctatt atagagtata aagaattatt aaaaaaaatt gaacttatag atattataga
     1861 taattataat ttctcaaggg agccagtaaa cactaaaaat ttaggtaaaa aaatgattga
     1921 taatgggaat ttatcaaatt taaaagaatt attacttgat aatataagtt ttttaaagca
     1981 atcaactgga acttctcgag aaatggattt tgtatctagc tcatataata tcggtttata
     2041 tataaaattg tgttggattg tagatggagg ggaggagtat aaaatgaaaa actatatgga
     2101 aagtaattta atatattcaa gagtaggtaa agacttattt aataaaatga cagatgaatc
     2161 cagaagaaaa aatttttcta tgaaagtgat tcaactactt aagtctaatg atagaactca
     2221 gctatttcaa actataatgc atgtgttagt ttctaatgga atcttaatag gagaagggtt
     2281 tgtagaaggt ctaatgcaga gttctgaatt agaattaaat acgaatgtag gtctatttat
     2341 tcaagaaatc atgaaataaa aaagggggaa aatttaaaat gaatgaaaaa agagtaaaag
     2401 gagtaacatt aactatactg acagaaagcc cagttccact ttcatatgac caagggtatg
     2461 gaaattatac accaataaaa aaagaacaat atagagaaaa aatacatgca aaaactagta
     2521 tagccacaat aacatatgat ttaagaagaa tgctccacca agaatatggt tggaatttaa
     2581 gtaatattgt ttttggaaaa aaaggaaata tatatccatc tattaagaaa aatgtaggat
     2641 gtgtagatga aaatggatta gagactgatg tatttggtta tttgatacca cttaaagata
     2701 aaggttctat aagtaaagct tctccactta gaataatacc ttttaggtca ttaaatccat
     2761 ataaaggttc aacacagcta ataacaaata gaggtttttt aagtagtgaa tttggtagaa
     2821 aatattatga tgagaaggaa gaaaatgaag tacctagaga tgaaaacttt ccaacatcac
     2881 aagcattagc aatagaagag actttatcag attactacgt ttatacaata acattggagc
     2941 ttgatagaat aggagtagta gaagtagaag atggaaagtt acttttacca gaagaaagaa
     3001 aatttatgag taaggagtta agagaaaaag cagtaaaaga tatattagat gcaattactg
     3061 tgtttacaag aaacataaaa cattcatcag tactattaaa accacttgca gtaatgggtg
     3121 gagcttttga taaagttgta ccgttttttt gggatgatgt agattataat gctgatagtg
     3181 gtgagataaa tttagaggga gttatagaaa ctatagaaag ttatagtctt aaagaaagta
     3241 atacaatact tgctatcaat gacagattaa aaatttctaa taaaaaggaa ttaaataagt
     3301 ttaatttaag taaataccct gtaaaagaga ttaaaaatct agcagataga ttggaaattg
     3361 gagaagataa tatgtggtat ttaaaagagt aggaggtttt ttgaattatg aaagtattac
     3421 aatgtaaact taagcaacca actgctcact atagagaccc aaaagtattt caaaatgaat
     3481 atataagtac tttaaattta ccatctaaga ccacaataat gggaatgata acgtatttat
     3541 gtgatagaag attaaactct gatatagata taggaataat tggaacacat catcatagag
     3601 aactagaatt ttcaagagga gagaacattg atttttggaa tgaatataca aatatgagaa
     3661 aaggcaaaga taaagaaaag tttttgcttc aaggaaacta ctatgattac tataaagaac
     3721 ataaagctca aaatagcata ttaaactatg aagttcttaa agaagtagaa ttgactatat
     3781 ttatttcttg caaggatgat gaagaacttg aatttattag aaaaaagctt gaaagtccat
     3841 gtaaatatgc aaatttagga agaaaagaag attttgtaat accaagtgaa aaagggtgtt
     3901 ttgtaaaaga agttgcgtta aaagaggtta tacctatgaa tactcgtgat gctataaaag
     3961 aaaatataaa attaaagaat acatatgtaa gagttgactt aagagataaa gagaatgtcg
     4021 agactataat aaatcaaggt gtattgattg cattgcctca taaatataaa gatttagaag
     4081 caaatagaga tgatagagtt tatgagtttt gccattatat atatgttgat aatgatggta
     4141 tatatcctaa aaatattaag gtaaatgtat gtattgatac aaaagaagtt tttacatggc
     4201 tttaaattat tttgaaagat aggtgtttat catgaaaaat atcatgcaaa aagaaatatt
     4261 agctaagtca tacaatttaa gaactaatca acaagaacaa acattagttg aacatattaa
     4321 ggatttattt gaagtattag aatctatatt agaactaaat ctatattcag ataaagatgt
     4381 agagatacta aaaatatgtt gtgctttgca cgatttagga aaaattaatt caattatgca
     4441 acaaaaaatg gagattaata ataaaatatc gtatagttgt tcagaagaag agaggaaaaa
     4501 gttagagtct gacaaaaaat ctttaaaaaa gatagcaaga cacaatatat tttctggagc
     4561 atttttaaaa aatatactag aaaaaatgaa cttgtcagaa gaagataagt tatatatata
     4621 taaaagtata atgttacatc atggaaacta tgaagattat atgaggttaa gtactagtaa
     4681 agttcaaaaa gaaatttatg attatattga aaaaggtatt ttagaaaaag aagattttaa
     4741 tttggaagat atagaagatt atataaagga gattttatat gtagatttta aatttaatga
     4801 agatatattg gattatgatt acatagataa attaagtgaa agtatggtta tatacagtga
     4861 ttataacaat ggtcaaatag aagattcagt tttaaattat agaaagttta agtatatttt
     4921 atataaaggg atgctgaact taatagacca tagtgcatca agtagacaga aaggtataaa
     4981 attttataac gattttactg atgaagaaat tgataatatg attttaaatg agatatataa
     5041 gagtcagggt aatttagaaa aaaataatat agagttcaat actatacaaa aaaggttgag
     5101 agaactttct ggaagaaatg ttctgactgt tgcatttaca ggttcaggaa aaacagctgc
     5161 agattataga aagactttta aaagaaaatt ctttttagtg ccaaataaaa tatcagcaga
     5221 aagtttttat agaaaaaata tttttcaaaa taaaaatgat ttagatacaa gaagtagtaa
     5281 tgattacatt ggattacttc atggagatat aaacttatat tcagagaatg aagataatgg
     5341 agaacatgat tttgtattga cattaaggga tatagatttg agtataaact tttgcaaacc
     5401 ttgtgtaatt gcaacagttg atcagttatt acttagtatg tttaaatttc cgggatatga
     5461 aaaaatattc gcagccgtaa aaaatgcatc tataactgtt gatgaagtac atttattgga
     5521 accaaagatg tttttgattt tgatatattt tatgcagttt gcatgcaagt acttagatgt
     5581 tgattttcac ttaatgacag ctacacttcc aaaatcatat aaagagcaaa tgataaacaa
     5641 gggaattatt tttcaagaag aaagtaatga aaatgttact gataaaggag aaatagtttt
     5701 tattgagagc aacaaggaag aagagatttg tgaaggtaaa gatgtaaagg tttcttttat
     5761 aaaggaaaaa gaaatcaagt caatagttga agatgcttta gaaaataaac aaaaaattct
     5821 tataataaag aatactattg attcagtcaa tagaacatat gagtttttaa aagaaaattt
     5881 atctgataag tatggtggtg tagatataga tgttttacat agtagattta agttcaaaga
     5941 taaaaaagag aaatatagta aaatattaaa tggagagggt gatatttgga tatcaactca
     6001 atcagtagaa atatccttgg atttggattt caatataata ataagtgatt tagcaactat
     6061 ggatagctta atacaaagaa tgggtagatg caatagaaat aataaatatg aatatggaaa
     6121 cttttatatt ctacctagtg aagataaaat ttatgacaac aaacttaaga gcacaactaa
     6181 aaacatctta aaggacatat taaaaactaa ctctattttt acaatgggaa ttagaaaaac
     6241 tattttggat gattactatg ataatagtgt agtcaaaaaa tattttgaag agaattttat
     6301 aagttgtgat aaagaaatta agaatattta tggtgtaaat aaagaaattt ttgatggatt
     6361 ggatttaatt tttaattttg aaccttacaa aaatattgtt gatagtaaga aggaagcagc
     6421 taaaattttt agagatgttg atgttagtta caaaattatt ctggaagaag atttttataa
     6481 ggaagataga gagttacaac aagattcgat tcaagtatca gggtttatat ttaatagatt
     6541 atattattat gggttaataa gcaaagtaga aggatacatg gtactaaaat ctagtagtaa
     6601 gtttgagtat aatagtactg taggcttaat tttgaagtga gcatatgttt aaatataata
     6661 tacctttaga cattgaaaat acaaaaagta ctactgtaag ctcactgtaa aaactatttt
     6721 tttaaaagga aaaataagtt gtgagataga gtgtttacaa gtgtatatag ataataaatt
     6781 tgaggtttaa gattaactaa gtgggtttca ggtacatcgc gaaaataatt ataagttgga
     6841 gattttgtat gtttaagatt aactaagtgg atttcaaata ttgatatagt agatataata
     6901 gaagttttat tattaggttt aaaattaact aatcggattt ttaaatattc tgactattat
     6961 ataaggatat tattagcttg aatttaaaac caataatata aaacaacaaa atattatatt
     7021 tatgatataa taaaagttat ggaaaaacag gaatattact atagagaaga gaggtaaact
     7081 tatgaataat tggatagaag taactataaa aacaacaaca gaagcagttg aacctataac
     7141 gaatatacta tatgaacaag gagctggagg agctgtaata gaagacccaa aagacttttt
     7201 attccaaaag aaaaatgaac tagactggga ttatgttgaa gaagaagtat ttaaaaagaa
     7261 tgaagaagat gatgttctta taaaaactta tgtttctgaa gaaaaaaatg tgatggaatt
     7321 tgtcgaaata ataaagcaaa aagttttagg attaaaagat tttggtatag atataggtga
     7381 aggaagtgta tcattggacc aagtaaatga agctgattgg gcaaatgcat ggaaagcata
     7441 ctacaaacca acaaaagtgg gacaaagggt agtagtaaaa cctacatggg aagattatgc
     7501 tatgcaagag ggggatttaa tcatagagct tgaccctgga atagcatttg gtacagggac
     7561 acatgagact actagtatgt gcattagaga attagaaaag tatgtgaata aagattctaa
     7621 agtatttgat attggatgtg gtagtggaat ccttgcaata gcagcagcca aattaggagc
     7681 aaaagaagtt gtagctgtag atttagatga agtggctgta aaagttgcaa aagaaaatgt
     7741 tcttgaaaat aaagttgaaa aaagtgtatc tgtaatgcat ggtaatttaa ccgatgtaat
     7801 aaaagataaa gcggatgtaa tagttgcaaa tataatagct gatataataa agatacttgc
     7861 aaaagatgtt caaaacttta tgaaagaaga tgctatattc atatcttcag gtataatttt
     7921 ggataaagta gaagaagtta aagaaagctt aattgaaaat ggatttgaaa tagttgaagt
     7981 tcaaaaatta ggtgaatggt cagcaatagt ttctaagctt aaaaaataag ggatatggtg
     8041 acgtataatg gatagatttt ttgttgaaaa aaataatata aatctacaag ataaaacttg
     8101 cactatagaa ggtgaagatg taaaacatat ttcaaaagtt ttaagatgca aattaggtga
     8161 gaaacttgaa atatgtgaca agaataataa tgagtatatt tgtgagataa tgaatataga
     8221 taagagtata gttaatttag agattttaga aaaagttgat ataaatagag aatcagaact
     8281 taaagtaagg ctataccagg gacttccaaa agctccaaaa atggaaatga tacttcaaaa
     8341 attaactgaa gtgggtgtag aagaaataat attagttcaa actaagagaa gtgttgttaa
     8401 agttgatgac aaaaaagaag ataaaaaatt tgaacgttgg gaaagaataa tttatgaagc
     8461 agcaaagcaa agcaaaagag gtaagatacc aaaattaaga ggtgttttaa gttttaaaga
     8521 agctttagaa gatatgaaaa aaaataatgt aaatatttgc ccttatgaaa atgaaagaac
     8581 agtttcaata aaacatgcac taaaaaaatg tgattctaat atagatagtg ttggaatttt
     8641 tataggtcca gaaggtggat tttctgaaga agaaatagaa caaatacaaa aaaataattg
     8701 taatgttgta tcattggggc ctagaatatt aagaactgag acagcttctg ttgtagcatc
     8761 aacaatagct ttgtatgaac taagtgattt aggaggagaa aaataaattg aaaaaagtag
     8821 ctttttatac acttgggtgt aaagttaatc aatatgaaac agaagctatg ctagaactat
     8881 ttgagaaaga tggttatgaa caagtcaata gtgaagagta tgctgatgtt tatgttataa
     8941 atacatgtac agttactcat atgagtgata gaaaatctcg tcaatatata agacgagtta
     9001 aaaagaagaa tccagatgca ataatagccg ttgtaggatg ttattctcag gtatctccgg
     9061 aagaaatttt ggatatagaa gaagtaaatt tagtcatggg tacaaatgat agaagaaaga
     9121 ttgttgaaga aattaaaaag ataaattcaa gtaagaaagt tagtacagtt gacgacataa
     9181 tgaaagttaa agcttttgaa gaaatagaaa tcagccaaac taatggaaag actagagcat
     9241 ttatgaaaat acaagatgga tgtgacagat tttgtactta ttgtatcatt ccttatgccc
     9301 gaggacgcgt tagaagtaga gatattgata gtatagtcga tgaagtgaaa aaattggcta
     9361 ataatggcta taaagaggtt gtattaacag gaatacatgt tgcctcttat ggaaaagatt
     9421 taaaggatag ggatattaaa cttcttgatg taataaaaca aataaaccag atagaaaaaa
     9481 tagaaagaat taggcttagt tctgttgaac caattttatt tacagatgaa tttgtaaatg
     9541 aagttttaaa gatggataaa gtttgtcctc attaccattt atcacttcaa agtggatgtg
     9601 atgaaactct aaaaagaatg aatagacgat acacaacttt agagtacaaa actattgtag
     9661 atagattgag aagtaaaatg ccagatgtgg caataactac agatgtaata gtaggttttc
     9721 ctggagagac aaatgaagag tttaaaaaaa cgtacgagtt tttaaaagaa atagagcttt
     9781 cacaaatgca tatttttaaa tattcaccaa gaaaaggaac tccagctgca actatggaaa
     9841 atcaagttga ccctcaaatg aaacacttta gaagtgaaca acttttaaat ttaagtaaag
     9901 taaattttaa taaatttgca actaaattta tcggccgtga gttggatgtt ttgtttgaac
     9961 aaaatataga aggcaataaa tatgagggat taacttcaaa ttatataaga gttgttgttg
    10021 aaagtgacaa aaatatacaa ggtcagatat taaaagtgaa aataaatgat gtaaaagatg
    10081 aatatgtaga aggaattttg ctataacatg tagaaattat aatatttata agataggagg
    10141 tggaaagaat ggattgtatt ttttgtaaaa tagcgaacgg agaaatacct tcaactaaag
    10201 tttatgaaga tgatagagtc ttagctttta atgacttgaa cccagttgct ccatatcata
    10261 tattagttgt acctaaaaaa cattatgata gtttaattga tattcctgat aaagagatgg
    10321 acattgtgtc acatattcat gttgttatta ataaaatagc taaagagaaa ggttttgacc
    10381 aaactggatt cagagtcata aataattgtg gaagtgatgg agggcaagaa gttaaacatt
    10441 tgcactatca tattttagct ggtaaaaagc ttcctaatta cgaagcggga caaaactaaa
    10501 ctaaattatt gaaaaattga gttccttctt gataaaaata cttgataaaa gtaaaaattt
    10561 agtttataat aaacaaagtg ttaggttgta atgcatatta tgcaaaaaga acatagttac
    10621 agtcccagca taattgcgct attggaggga gggaaaaaga aatgtcagaa gtaagagtta
    10681 gagagaatga aacattagac agcgctttaa gaagattcaa gcgtcaatgt gcaatgtctg
    10741 gcatcatgtc agaagtgaga aaaagagagc actatgataa gccaagcgtt aagcgtaaga
    10801 aaaaagctga agcagcaaga agaaaaaacg ctaagaaata gtagtaaata agaataaaga
    10861 ggtgaagggg atgtccctta agcaaaagtt acaagaggat ttgaaatctt cgatgaaaaa
    10921 caaagataca gtgagaaaat ctgtagtaac tttaataaga gcttctataa agcagtatga
    10981 ggttgataat agggtcgagc ttgatgaaga tggaattatc gatgtaatag caaaacaatt
    11041 aaagcaacgt agagatgcat tagttgaatt tgaaaaagct ggaagagaag acttaataaa
    11101 agaaacagaa ggtgaaattg aagttttaaa agagtaccta cctcaacaat taagcgaaga
    11161 agagttagaa gaaattgtaa aatctactat atctgaagta ggggcaactt ctatgaagga
    11221 tatgggaaaa ataatgtcag ttatccaacc aaaagttaaa ggaagagctg acggaaaact
    11281 tataaataag ttggtaaaac aaaacttaca atagattgat atagcctaga gagaaatctc
    11341 taggcttttt atagtttttg ggaataacga gtcatatttg agcatatcta taatcaaaga
    11401 aaattttgaa agaaggggaa aaatttgatt atatcagtaa aagagattag gaattttttg
    11461 ataaacatat ttataccact aataataggt tacttaagta gcatcttaac aatgattatc
    11521 agtggaacag atatttcaac ctattattta caactcacaa agccaagctt tgcaccacca
    11581 gcttttatct ttcctatagt ttggactata ttgtacatat tgatgggtat atcttcatac
    11641 ctaatattaa gaaagggtta caacttacct aaagtaagag atgcaatttt ttattatgga
    11701 ttacaacttg tattaaactt tatttggagt atactatttt ttggatttgg acttagattt
    11761 acagctttga tagatataat aataatgatt ttggtttcta taataatgat ttcgaagttt
    11821 tctaaaatag ataagagatc tggatacata aatttgattt atttaatgtg gcttgtttat
    11881 gcaggatttt tgaactattt tatatggttt attaataaat agaatgtaaa gattttgtaa
    11941 attaccctgt acttttaata atatcataac aaacgaaaaa taaatagact attaaaaata
    12001 atcattttca tatacaataa tattaattta aatacaataa atttataaaa attgtattta
    12061 aagattaaca tattaaagta ttagaattgg aaaggcttga agggagaggt ttttatgcaa
    12121 ataatagtat ctgggagaca aatgaaatta actgacggaa taaaaggata cgtagatgga
    12181 aaattaagta ggttagaaaa ataccttgat cctgaatcag aagtgaaagt aacagtaagt
    12241 gcgaaaaagg atagacaaaa agtggaggtt acaataattc ctattaacgg tcaaataata
    12301 agagcagaag atgtagaaga tgacttatat gcagcaattg atattgtttg tgacaaatta
    12361 agtagacaag ttgtcaaata taaaactaaa gttaaagaca aagttcaaaa taataagagc
    12421 ataagatttg aaaatcttga ctttatagat aatagcagtg aatttgatga ttatgattat
    12481 gatgatgaag aagatgaaaa tatagtaata gaaagaagaa agaaatttaa tgtaaaacct
    12541 atgagttctg aggaggcgat acttcaaatg gaactagtag gacacaattt ctatatgttt
    12601 agaaaccaag ataattttga aataaacata gtgtataagc gtaaagctgg tggatatggt
    12661 ttaatagaac aagattaata aaaattgtat acgtttagat tttatataag aaaaaataaa
    12721 aaggtaatca tgttgatttt ttatcaatat gattaccttt ttcttgttta aataatatat
    12781 tagatattag gtatttatta tataaaaatt ttaaaactaa tctgttacaa catcaataaa
    12841 gtgcataaat acctatttca agcataaaat taattaaggg ggtaatgctt atgttagaaa
    12901 tatccactga tttacaagta aatcaaccag ttattactgt tacaagtaat acgtttatta
    12961 gtatagaaaa ttatctatca atattagaat atgaagttga tttaataaga ataaagacta
    13021 aagttaaaac aattaaaata tctggggata aactttcact taagtatatt actgatagtg
    13081 agataggtat aaaagggatt atctacaatg ttgagtatgt tgattaggag gagatacact
    13141 tgataagttt tataagggga tactatgtta tagttgtgga aggagtaaga ctcgaacaat
    13201 ttttaaatca tcttataaga aatgggataa gtgtttacaa tgtaactaga ataaaaaata
    13261 ctaaaatgga gtttcatata gatagacaag atataaaaga atttaagaat gtttatagag
    13321 gaagtaagtt tgatataaag gtaaagcaaa aaactggggt cccttttatt atcaaaagag
    13381 tttataagca caaaggtatg tggatttgtg ctcttgtttc tttattttta ttgatgtcta
    13441 catcacagtt tgtgacagat gtatatatac aatctcctga aggtataaaa aaagaagctc
    13501 ttagaaatga gctttataag gttggagtaa gaccaggagt ctataagaag agtattgata
    13561 gaaaagaagt tagagaccat atgatgtcaa aatttaatga tgtggcatat ttatctatca
    13621 atgtaaaagg aacaaatata tttgttactg ttacaaaaaa ggcagaatcc ctaaaatcta
    13681 cagaccaatc aaattattgt aatgtaattg ctttaaaaaa tggtataata gaaaaggtaa
    13741 tccctagaag tggtaaatca gttattaaat ctggagacat agtccaaaag ggagatgtct
    13801 tattaaatgg agctaatacc aagtcaatac cagaagtatg ggcaagtact ttttatgagt
    13861 ccacaaaaaa ggcaagttat gtagatactg taaataaaaa aactggagaa aagaaaaata
    13921 tatatacact aagtttttat gataaagagt ttacaatgag aaaaaatata aaatataaag
    13981 attatgttgt tgaaaataaa gaaaaaaaac tatcaatagg aaactatact tttccaataa
    14041 agataaaaac gagtactttt tatgaaacaa aaaaggttga agtaaaaagg aataaagaag
    14101 agttaaaaaa agaattatct gaaaaagctt taaaagagtt agaatacata atcccagctt
    14161 cggctagaat aatagatgta aaacataact ttaaggttaa taaaaatatg ttagaatatt
    14221 taataacagt tcaaactagt gaaaatattg ctaaaattta tcctcttagt aagtcagaag
    14281 cagaaaggtt tataaaggaa gaaagtaaac cagatgaagg tgaagaggaa gttccttcca
    14341 atccagaaaa aagaccatta aatgatataa gaaatgagtt tgatgaggat aataaggata
    14401 ataaagacca aaacaattct gatgaaaata atagtaatca aaatagtaat aatagtcaaa
    14461 ataataatag caataacaac taataaaatt aaacttaaaa tcttaggagg actaattcaa
    14521 ttgataatac aaaagaaatt tacaatagca gatggtaact ttgaaagaga gttatttggc
    14581 aactttgatg aaaatgtaaa attaatagag aagactttaa atatagatgt aatacttaga
    14641 gaaggcaata taattttaat tggagaagaa aaaaatgtag attctgcatt aaaacttatg
    14701 aatgaattac accaaactgt atcaaatggt aagcatttag ataagcagag tatttcttat
    14761 tcattatctc ttttgcttga aggaagtgaa caaaaaataa aagaacttga ggggacaatc
    14821 gtcataactc aaaggggaaa agctgttcaa cctaaaactt taggtcaaaa agaatatata
    14881 aaacttatag aaaataatga tataactttt ggtgttggac ctgctggaac aggaaagact
    14941 tatcttgcgg ttgctatggc agtaaaagct tttaaaagag atgaagttag tagaattata
    15001 cttacaagac cagctgtgga agcaggagag agtctaggtt ttttaccagg agatttaaag
    15061 gacaaagttg acccatattt aagaccacta tatgatgcct tatttgaaat gttgggtgct
    15121 gataaattta ataagtattt agaaagaggt actattgagg ttgcaccact tgcatttatg
    15181 agaggtagaa cacttgataa ttcatttata atattggatg aagctcaaaa tactacttct
    15241 gaacaaatga aaatgttttt aactagactt ggatttggct ctaaagctgt agttacagga
    15301 gatgtaactc aaacagattt accacaaaat aagaagagtg gtctaataca agctacagag
    15361 attcttaaag gtgttccagg tattgggtct ataatgctaa cagatagaga tgttgtaaga
    15421 catgagttgg tacagagaat aataagagct tatgagaagc acgacaaaag agaagaattt
    15481 aaaaaagaag aacgtaaaaa agcgaaaatg caggaaaaga ctttcaaaag aaagtaaaag
    15541 tgtagtaata tagatatata gcattattaa tgaataaaaa gtaggtgtag tgaatggatt
    15601 tgatactaga tgatagacaa gataaattag aagtaagtga ggagttaatt gaaaagataa
    15661 aggatataat catagaatgt ttagattatg aaggatatga tgataattat gaagtgagtc
    15721 tttcttttgt agataataaa gagattcatg aattaaatag agaatataga ggagttgata
    15781 gagtgactga tgttttatca tttcctcttt taagtgatga ttttgaggat gttgagttag
    15841 aagaagaatc cctaggtgac atagtagtat ctcttgaaag agccttagaa cagagtatag
    15901 agtataacca tagttttgaa agagaagtat gttttttaat atgtcatagt atgtttcatc
    15961 ttttaggtta tgaccacgat acagatgaaa atacaaaaga aatgagagaa aaagaagaac
    16021 acatattaaa taagttaaac ataacaaggg agtagttttt atgaaaccag aaaaaactcg
    16081 tcaaggtata attaaagcat ttaatgcagc tatagaaggg atattgtata catttaaatt
    16141 tgaaagaaat atgaaaatac attatttagg ttcagttgct gttcttataa taagtttgtt
    16201 ttttaacttt tcaaaattag aaatgataat gttacttatg tccatatgct tagttgtagt
    16261 tgctgaaatg tttaatacag ctatagaaaa agctgttgat ttagtaactg atgaatatca
    16321 tgttcttgct aagatagcaa aagatgttgc agctggaggc gttctagttg cagcactaaa
    16381 ttcagtagta gttggatata ttttgtttta tgataaattg acagatatat ctggaatatt
    16441 aatttataaa ataagagaat cagaattaca tataaccttg atatgtatat tattggttct
    16501 tatagctgtt gtagttgtaa aagcattgac ttctacaggt actcctctaa aaggaggaat
    16561 gccaagtggt catgctgcct tagcatttgc tatagcaact gcaattacac ttatgactga
    16621 aagagttgtt gcctctactc ttgcatatat aatggctgta ttagttgccc aaagtagaat
    16681 tgaaggaaaa atacatacct tttgggagac tattgctgga gctttgttag gtgtgttgat
    16741 agctatatta gtatttcagc ttggaatgtt ctataattaa aataaatgta cataaaatat
    16801 ttaaaataaa acaaatctgt gctaattaaa tttttatgat ataatgtaga gtattaaata
    16861 aaaataactt ttaattattg tatacaggag gagttgttgt ggataacata gaattgttaa
    16921 ggttggcaga agatgccaga caacattctt atgctcctta ctcaggtttt agagtaggtg
    16981 cagctctttt aactaagagt ggtaaagttt atactggatg taatatagaa tgtgcatcat
    17041 taggtggaac aaattgtgca gaaagaacag ctatttttaa agcaatatca gagggtgata
    17101 aggatatcta taaaatagct atagcaagtg ataactcaga aaataatgag caaacatatc
    17161 catgtggaat atgcaggcag gtaattatag agtttggttc tgatataaaa ataataacgg
    17221 gatatactaa aggagaggtg tttgagcata gtataaagga tttgttgcca aactatttct
    17281 ctggaaatga tttaaaataa atttaaaagt ataaataaat ttaaaagtat aaataaattt
    17341 aaaagtataa ataaatttaa gtaattaaaa caagaagata aaaagttagg agacagttaa
    17401 tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa tgtaggaaaa tctactttaa
    17461 tgaataatgt agttggagaa aaaatagcta taatgagtga taagcctcaa actacaagaa
    17521 atacaataca agcagtatat acagatgaag aaatgcagat agtattctta gatacaccag
    17581 gtattcacaa accaaaaaat aaattaggtg aatttatggt aaaggcagca acagaagctt
    17641 ttaaaaatgt agatttaata ttatttgtag ttgatgattc taaaaaaata ggtcctggag
    17701 atagaaagat aatagaggat ttaagaagtg ttaagacacc aataatttta gttgtaaaca
    17761 aaatagacca actagaccaa aaagatgagc tatttgatat aatcaaaatg tatgatagag
    17821 aaggtatttt taaagaaata gtaccaatat cagcattaaa aggaaagaat acggatacct
    17881 taataaaagt tatacaaaat tatttagaag aagggccaaa gtattttcca gattatatga
    17941 ttacagatca gccagaaaga gtattaatag cagaacttat aagagaaaaa gttcttcact
    18001 atcttaatga tgaaatacct catggtgtag ctgttgaaat tgaaaaaatg aaagcgagaa
    18061 atgataagga aatagtagat gtatctgctg taatatactg tgaaagagat tcacataagg
    18121 gaataataat aggaaaaaat ggtaggaaac taaaaggaat aggtaagtca gcaagacaag
    18181 atatagaatt gcttttaggt tcacaaataa atcttcaatt atgggttaaa gtcaaagaaa
    18241 attggagaaa tttacaaaat tatataaata attttggata taatgataaa taaaggtggg
    18301 ttatatggat aggaaactgg atgcttccca agatttatta tatgaagtaa aatcattaat
    18361 tgataataat aaagtattgg agttaagaga attaatagag gaatatcata taattgatat
    18421 attcgatata atggaaaatt tagaggaaga catgaaaatt caattatttg aagtacttcc
    18481 tttagatatg gcatcttcta tattagaaga aggtagtgta gagtttttta ttagtatttt
    18541 atctaaactg gatgtagaac actcgaaaaa tatacttgaa cttatgtctc ttggagatat
    18601 ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa cacataataa atcttttgaa
    18661 tcaagagaat gccgattatg taaaagagtt actattctat gatgaagatt ctgctggtgg
    18721 aacaatgacc actggatata tttctataaa taaagatatg acagctcttg aggctataga
    18781 ccatatgaga gaagaagctg aagaagctga aactatttat tatatatatg tggttgatga
    18841 tgaagaaaaa ctagttggag tattgtcatt aagagaatta ataatagcta gagacgcaaa
    18901 tatagtagaa gatttgatga gtgaaaatat aatctctgtt tatgtagatg aagatagaga
    18961 agaagcagta agattagtat ctaaatacaa tttaatagca attccagtag tagacagaca
    19021 agaaaaatta aaaggaataa taacagtaga tgatataatt gatgtaatgg aagaagaagc
    19081 aactgaagat atgtataaat ttgcaggaag ttcagaacat gaaagagaag ttgctgaaaa
    19141 agaaaaccct acattgagag agcaaataat atcagcactt agaggacgat taccttggtt
    19201 aattattacc ttagttggag ggttgttggc ttcgttaata ctttctaact tagactatat
    19261 aatgaatcct gtatatgctc cattagtatt ttttatacct gtagtaattg gtatgggtgg
    19321 aaatattgga actcaatcat catctgttac agtaataaca ttatctaata aggatttaaa
    19381 ttttagcaat gtggttagag aaggtattgt aggtattatt acaggtcttt tgtgtagtat
    19441 aataacaggt attgtaattt actttgttat gagaaagtta gacattgtat taattgtttc
    19501 aatatcatta tttataaata tggttttagg tgcaacaata ggtgcgttta tgcctgtttt
    19561 attaaaaaaa atggatgcag acccatctac agtttcatca ccaattatat ctacagctct
    19621 tgatataact ggtatagcag tttactttat aataacaacg gcattattgt caaaaattgt
    19681 ttaataactt tatgtatgat ttatcctctt cttactaaaa atataagtat aaatgtaaga
    19741 agggggtttt ttaaaaatga acaatatcag ttgggaagtt tttaaaaaaa caggtagtat
    19801 agatgcatat ttatatttct gtgattgcaa aaacatacat gaagaaattg aagaagtaag
    19861 ggagaaaaaa gaagacgatg ataatcctga acacccaggg gatagtactt aaagctataa
    19921 ggtataagga aagtgacata atacttacac tatttacaag aaagcttgga aaagtatctg
    19981 caatagcaaa aggagctaaa aaaaataaga gctctttgct ttcatcatca cagttatttt
    20041 cttatagcaa ttttacctta aaaaaacaag gtaatatgta caaggttact caaagtgaaa
    20101 taataaaaag tttttataat atatcctatg atatagaggc attttcttat gctacatata
    20161 ttacaaaatt agttgaaaat tctatacttg aaaatcagac taataatagg ctatttatat
    20221 tacttgcgca gactctgtat ttgtatacac aggataatac agacaatagg tttataacag
    20281 cagcattcga gttaaaattt ttagactata taggatttaa acctatagta aa
//
LOCUS       DAKBNN010000053        20297 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000053 DAKBNN010000000
VERSION     DAKBNN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.053"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..1358)
                     /locus_tag="OUB02_001659"
     CDS             complement(<1..1358)
                     /locus_tag="OUB02_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCT8885421.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKCP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDP"
     gene            complement(1369..2589)
                     /locus_tag="OUB02_001660"
     CDS             complement(1369..2589)
                     /locus_tag="OUB02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCT8885422.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2603..3391)
                     /locus_tag="OUB02_001661"
     CDS             complement(2603..3391)
                     /locus_tag="OUB02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCT8885423.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3408..4682)
                     /gene="larA"
                     /locus_tag="OUB02_001662"
     CDS             complement(3408..4682)
                     /gene="larA"
                     /locus_tag="OUB02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HCT8885424.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4800..5513)
                     /locus_tag="OUB02_001663"
     CDS             complement(4800..5513)
                     /locus_tag="OUB02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCT8885425.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5638..6897
                     /locus_tag="OUB02_001664"
     CDS             5638..6897
                     /locus_tag="OUB02_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HCT8885426.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTIIQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7165..7731
                     /locus_tag="OUB02_001665"
     CDS             7165..7731
                     /locus_tag="OUB02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8885427.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTYSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7932..9137)
                     /locus_tag="OUB02_001666"
     CDS             complement(7932..9137)
                     /locus_tag="OUB02_001666"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCT8885428.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9240..9346)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9438..9806)
                     /locus_tag="OUB02_001667"
     CDS             complement(9438..9806)
                     /locus_tag="OUB02_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HCT8885429.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9858..11135)
                     /gene="obgE"
                     /locus_tag="OUB02_001668"
     CDS             complement(9858..11135)
                     /gene="obgE"
                     /locus_tag="OUB02_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCT8885430.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11246..11536)
                     /gene="rpmA"
                     /locus_tag="OUB02_001669"
     CDS             complement(11246..11536)
                     /gene="rpmA"
                     /locus_tag="OUB02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCT8885431.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11550..11867)
                     /locus_tag="OUB02_001670"
     CDS             complement(11550..11867)
                     /locus_tag="OUB02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCT8885432.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11882..12193)
                     /gene="rplU"
                     /locus_tag="OUB02_001671"
     CDS             complement(11882..12193)
                     /gene="rplU"
                     /locus_tag="OUB02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCT8885433.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12294..13715)
                     /locus_tag="OUB02_001672"
     CDS             complement(12294..13715)
                     /locus_tag="OUB02_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HCT8885434.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13733..14434)
                     /locus_tag="OUB02_001673"
     CDS             complement(13733..14434)
                     /locus_tag="OUB02_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HCT8885435.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14436..16280)
                     /locus_tag="OUB02_001674"
     CDS             complement(14436..16280)
                     /locus_tag="OUB02_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HCT8885436.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16599..17792
                     /locus_tag="OUB02_001675"
     CDS             16599..17792
                     /locus_tag="OUB02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HCT8885437.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18004..18825)
                     /locus_tag="OUB02_001676"
                     /pseudo
     CDS             complement(18004..18825)
                     /locus_tag="OUB02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(18955..19746)
                     /locus_tag="OUB02_001677"
     CDS             complement(18955..19746)
                     /locus_tag="OUB02_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885438.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(19750..20079)
                     /locus_tag="OUB02_001678"
     CDS             complement(19750..20079)
                     /locus_tag="OUB02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885439.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6382 a         3697 c         2251 g         7967 t
ORIGIN      
        1 gggtcaaaag ctaactttat atttttaatt aacatcatat aagcatctga atttgattca
       61 tgtaagtact cttttttagc atatcctatg ccatgttcac cagaaacttg accactaagt
      121 tctctagatt ttttatacat atagtcaaaa gtttctttta atcttatttt ccatgtatta
      181 tcatccatac catcttttaa aatatatatg tgtagatttc catctcctgc atgaccaaaa
      241 ctttttattc ttatttttaa tttgtcttga agctcgtgag tgtatcttat aaactctgct
      301 atcttatctc ttggaacaac gacatcacat tcatccattt gtgtcgtaga tgcctttata
      361 gcttctaaaa atgcacctct ggctgaccat atagaatcgt ttctttcttg tgtatctgat
      421 ataaatacat ctaatgcacc attttctaaa cataaattag ctactttttc atattctttt
      481 tctatatctt cagtgctatt tccatcaaat gtaagcaaaa gataagcatc tgatgtatta
      541 tctggaaact ttttacctaa aaattcttct gctgccaata ttacatctct ttccataaat
      601 tctatagccg ttggtattga ttttgatttt attattttag gaacagtttc tattgccatt
      661 gataaatctg gaaatggtat aagcaaactt atacttttct ttggtaaagg aagcaatttt
      721 aatatagctt tagtaactat tcctaaagta ccttctgagc ccactaataa atcctttata
      781 ctatatccag aactattttt aaccaccttt ccacctacat ttataatttc tccatttggt
      841 agtacaactt caagacctct tacataatcc cttgtaacgc catattttac tgctctcata
      901 ccacctgcat tagtgtttat attacctgca attgtagcac ttttttcacc tgggtcaggt
      961 gggtaaaata aatctctatc ttgaacatat tgacctattg tcatcaataa aacacctggt
     1021 tctactgtca atgttaaatt ttcataatca acttcaagta ttttattcat tttacataga
     1081 ttaatcatta ttccaccatt tatggcaaca gcagcaccta caagaccagt accttgacct
     1141 cttggtgtga ctggtatatt atttttatat gcgtatttca ttattttaga tacttgttca
     1201 gtttcaagta catttactaa aacttcaggg cacttctcaa tccctccaag ttcatcatgt
     1261 gaaaaatctt catttatatc acttccaact aatacatttt cttctccaca agtatctatt
     1321 aaaaaatcta tatctttttt atcgattaat ttatacattt ctatccccct atttagctgc
     1381 tgatgccata tatttttgat tattatcttt gttattttct attttttcaa tcaatttagg
     1441 aataatatca taaatatcac ctattaaagc taaatgtgct acatcaagta taggcgcttt
     1501 atcatcttta tttattgcta ctatatagtc agcaccctgc atccccgcaa caaattgaac
     1561 agctccagat acaccacaag ttattatcaa tttaggtttt acagttctac cactaagacc
     1621 tatttgtttt ttagcatcta tccaacctgc ttctattaca ggtcttgttc ctgccacttg
     1681 agcgtttaac ttatcagcta atttatatat catctccata tcttcttgtt tttttattgc
     1741 tctacttgcc acaactatca cttcagcttc ttccagacca acttctttat ttttttcttt
     1801 tacactcagt accttaattt tagattttag ttcttttttt tctactttac ataaagttat
     1861 ctttcctgta gtattctcta ttttttctgg tgctgaaaat attttatatc ttacagtggc
     1921 aaattgtggt ctattattag gtgtgtttat atgagccatt atatttccac caaatgctgg
     1981 tcttatttgg tctaaatcag tatttgattg aatatcaagt attgtacaat ctgctgtaag
     2041 acctgttcta aatctagcgg caagtcttgg agctaatgac ctacctaatg tagttccacc
     2101 aactaatact atcgttggtt ttattttatc tataaagttt tctattgctt ttgaatatgg
     2161 ttctatccta aaatctttaa attcatcttc atcatataca aatacttcat ccaccccata
     2221 agaaatcaaa ttactgcact tatcttttat gtcttttcct acaaatacac aatacacagg
     2281 ttgttttatt tttgatgcca tctcacgagc tttacctata agctcgtacg ttacaggatg
     2341 tatatttcca ttatgatgtt caacatatac tgcaattccc ctccattcat ctttattaat
     2401 ttgtacttta ctactctcta taaattcaaa tgcgcctttt ggtccacttt tgacacacaa
     2461 cttgcacatc ttacagccag cacttatttc tactatacct gacttttctt ctatagcccc
     2521 aaaaggacat atttctaaaa ttctctttaa gtcagttatt ttatcttgat ttacaactat
     2581 ctttgacatt tattactccc ccttacacca actttaattc ctctaattta tttacaagtg
     2641 caaaacttag ttcatctgca tttcctctta caatattttt atctgtattt ggctttggag
     2701 gaaatattct tacaacttga gtaggagaac cattaagtcc atagttattt tcatctttat
     2761 cttcaaagtc atctaagcta tatacaggta tttctctatc tttagtatta agttttaatt
     2821 taaatgaagg tagtcttggt tgaaaaatgt ctttttctac agttattaaa catggatatt
     2881 caatctcaca tacttgtaaa tcatttggca tatctatttc aacaactata ctttcatctt
     2941 taacttcaat taattttgta acgttagtaa catgaggtat atctaaaaat tcagctactt
     3001 caggtcccac ctgtgctgta tcaccatcag tagtttgttt tccacaaatt attaggtcta
     3061 cctttcccat cttcttaata ccttgtgata tggtatagga agtagccaat acatctgctc
     3121 ctccaaatct cctatcactt attaatgctc cttcatcagc tcccatcata aaactttctt
     3181 caattacctt ctttgcctga ggaggaccca tgctaagtac ttttagattc gctttcttct
     3241 cttcctttat tctaagagct gtttctaaag catataggtc atagggattc atttttgaat
     3301 ctactccatc tctttttagt actcctgtag tttcatcaac ttctactgaa gtcgttccag
     3361 gaacttgttt aatacatgtc aaaatttcca taaagtacac tcctttttta tttaactata
     3421 actgatactc catcaggtat aactactact ttgctctcct taccaactat actattcgcc
     3481 atttctaaag cttctttaaa gttaaatgca tgtttcatat gcattttttt tatcatttca
     3541 ggattacact tatctgttac cattatcaca gtatatttag ataatattct cgctagtatc
     3601 tgaaattccc attggtctgg aatcgtatct actctatcga cacttctaat tttatctaaa
     3661 agttgttttg gagaatctgc atttgctaca ttttcataaa aagattgacc tccatgacca
     3721 tcattacatg ctgaaatcat tattattact ccaccgtctt tacaagtagc ttcagctgct
     3781 gtcattcctt taacagattg ataaatattt tggtctagtg gatatccacc atttgtagtt
     3841 acaacaatat cagattttat agaatttact tttgataact ctgttacaaa tctacatcct
     3901 ttagtatgtg ctttttcgac atcaccagca aaggaagcaa ttattttttt atctttatct
     3961 attacaacat tcaatatgaa tgctaatttt gcaactttag ctgcatatac catatcctca
     4021 tgaataggat tattattcaa ttttccagtt cttgaattat cactatctat aaagtcacta
     4081 cagtgattgt acattatagt ctttgcagat gcgattcctg gaagtacgct ttttctgcca
     4141 cctgaaaaac ctgcaaagaa atgaggttct ataaaacctt ctgctatcaa aagttctgcc
     4201 tcatatgcta gtttgtttat atataaatct ccacctgaag gcaatatccc tactttacac
     4261 atagatttat catcagtaga tatatgcata actatatctt cattatctac tatttcttct
     4321 cccattttat aaattagctc ttcccttgta cttggcctat gaaatccagt tgccactata
     4381 atcttcacat caatttctgg attaacattc ctaatccttc ttaatataat tggcatagtt
     4441 attctacttg gtacaggtct tgtatggtca cttgtaatta tgactacatt ctttttcccc
     4501 ttaaccagtt cttccagaga attagagcct ataggattat tcatagattc ttcaactatc
     4561 tcttcttgag tcttatgttc tatataatta tccgattttg actccaaaac accgagaaaa
     4621 cgttcatcag gtatttctaa aatcattcct tgttttgaat acggtacttt taactttacc
     4681 atttttaatc tcccattata ataaactata aaattggtat gtggtcatac cagttttaca
     4741 tttatatgat agtaccatta tctaccattt gcaagaataa tttattttat aacaaacttt
     4801 taattttcat tttttctata atttttactt ataaaatcca tatgttcctt tgctttctca
     4861 catgcaagtt ctacatcatt ttctacaact gcattgtata tagcttcatg ctgtttaagc
     4921 aatatatttt cattaaaagg ttctgcgatt attttttctc ttgcatcttc tataaatcca
     4981 tcaaataatt ttgatgctgt cataaaaaga ctttgaatta aataattttt acttaatgtt
     5041 gctagtttac tgtgaaattt ttggtctaat aacaccagta ctcgattatt atctttttta
     5101 ttagtttcta ccctcatttc atctattata tgtttcaatt ctataacttc agaagaagtt
     5161 attctctttg aagcaagttt tgcaatctca atttctagga ttattctaag ctctaatata
     5221 tcttcaagtg taccattatt aagtttaaaa atcatagata aaggttctat taaagttttt
     5281 tctatatttg tacatataaa atttccttca ccctgtctgc tttctatcac tcccatagtc
     5341 tctaataccc taatagcttc ccttatggaa gttctactca cattcatttg ttcagacagt
     5401 tctctttctg atagtaattt atcccccttt tttaactctc catttagtat tttatattgt
     5461 atttgctcaa ttacttgttc ataaactttt ttactagcta tactattaaa cattttttcc
     5521 tccaatatga taatcgttgt tgtaatatat atctattata tatcttaagc cattacattc
     5581 atatattttt attactttta atataatatc ttataactca aatttacagg tgatattatg
     5641 aaaaaaaata aaattattgc tttttttgct ccatctatca tagttggagt tctagtttta
     5701 gggattatcc tatacccaaa tgaagctata aaatctgcaa gagaaggctt ttctatatgg
     5761 tctaatgttt tagttccatc actactacca tttataattg gagcaaactt aattgttgat
     5821 ttaaaaatag tggatattat agggtttatc atcaatccca taactagatt tgtatttaat
     5881 gtttcaggaa agagcgcttt agcttttgct atatctacag tatctggtta tccagtagga
     5941 gtaagtttag cttctgaatt taggagtaat ggacaaattt ctaagtatga agcacaaaga
     6001 cttgtgtctt tttgttctac atcaggacct ttattcataa taggttcggt tggtacaggt
     6061 atgttccaaa attcaaactt aggttattta atgattctct gccattatct aggaactata
     6121 ttggttggtc ttttctttag aaattatggt aatgaaaacc ttccaaagac aaaattggat
     6181 ttaaaaacaa atataaaaaa tgtaatcaac attagaaatt caagtgggaa tggttttttt
     6241 gttttatttg gaaatgctgt atttaatgga gtaaatacat tacttacagt tggtggattt
     6301 gtaattgttt tttcagtagt atttaaaata ctatctcttt ttaatgtaat ctcattaata
     6361 gcatcagtta tatatttgcc tctttcattt ttaggtgtta gtaaagaact ctgtcaagca
     6421 tttgtaagtg gtctatttga aattactata ggatgcaata aagtatctag tataacaagt
     6481 tctccagaaa ttttaagagc atctttagct agttttttga taggatttag tggattatcc
     6541 atcctagctc aatgttgtac atttctatca aaaacggaca tagatttaaa attatacata
     6601 ctaagtaaat ttgcgcatgg agttttagct gctatcttta tatttatact ttatccaatt
     6661 gcaaactctg ctgtgttggt ttctagcttt gctgatacat acaacgttat atataataat
     6721 ttaatctggt tttattacct tagctattat gatacaatta ttcaaattgt agtaataatt
     6781 tatctaatct cagccgtatt tattgcaaaa aaacatatga aaactttatc tacaaatggg
     6841 aaactaatgc gatataaaaa tacttttttc actaagaaat tttcacgaaa aaaataaaac
     6901 taataaaact taactaaata cttccatttt tttattatat ttgtcataat tttaataacc
     6961 tttttaatta gttcaacagc atagttattt catcacaatt taataatttt attagaaata
     7021 tttttcaaaa aattctcctt aatcacatat atcacacttg tataagttta ctgggaaatt
     7081 tttccattat ataatatttc atttatatta tataataaat accgacctaa acttaccaaa
     7141 tcaaactaag aggagtggtt gaaaatggca acaagaccta tagaaataga cgaatataaa
     7201 aagataatgg aactattaca tactggcttt acttatagtg aaaatggcgt tgaaaaaaga
     7261 tttagaaaaa atcctaaagt tgcattggca ctaatgttag aagctaattt aggtctaaga
     7321 atatcagata ttttaagatt aaagataggt aattttaaag gtaatatgtt ggaaataaat
     7381 gaaaaaaaga cgggcaaatt acagtataga ccagttaata aaaatataat tgagtccata
     7441 aatgaacatg caagaaagta taatcttaaa tcaaatgact acttagtaaa tataaagaca
     7501 aaggctattc aaaaacagtt gaggataatt tgtaaatact taaatctgta taatataagt
     7561 acatatagct ttagaaagct ctatgctact actcaatttg aaaagagtaa caataattta
     7621 gagttggtta aagaactttt aaatcacagc tctgtagcca ctacacagcg atatataagg
     7681 gtaactcagc aagctattaa tgaagctagt gaaaatttct ttattggtta attataacgt
     7741 atacattttt ctattatctt attaattcac cagatattaa aaaagagtgt ctcaaaatga
     7801 actttttagt tcattagaca ctcttttttt ataaaatcaa atatattttt attatttcaa
     7861 cactggaaaa aaggcttatc tctctatttt gagacaccct attaaatttt cataattctt
     7921 gcataaacat attaatctat cttcaataat tttttagctg attcactgta attatttctt
     7981 tttacaattt ccaaaactct atcaatctca tgttctgact ttgcacttac aaaaatcgcc
     8041 aattcttcat catctataat atcataacta tctacatatt tatctaaaca ttctaaaatt
     8101 ccatctttat tattataccc accaaatcta actactgcac tacactcatc ttctataagt
     8161 ccacttaaat ccaatttccc ttttgaaaaa gactttatat ttgaacttct attatctatt
     8221 atatctataa tatctgcata aacagcactt cctgttgcac ctcttccagc tcccttacct
     8281 acaaaagaaa gttcacctac tgaatcacct actaaaataa ctacgttgaa ctcattatct
     8341 atctttgaaa gcatattatt attgtccact aatgctggtc taacaaatcc acttacttct
     8401 cctgattcat acttacttct agcaaccaat tttatcttac aatttaattt ttttgcatat
     8461 tctatatctt tttcatctat tgaagttatt ccttctaata ataaatcttt ccaatatact
     8521 atttttccat aagataaagt agacaatata gataactttc tagcactatc atatcctaaa
     8581 acatctgcat ctggattaac ttctgcaaat cccaactctt gtgcctgttt taaagcgaca
     8641 tcatacggta aattttcatc atacatcttt gataatataa aattacaagt cccatttaaa
     8701 atagcgttta tgctctttat attgttgcct tccagtgatt ctatcaatgg ttttaaaact
     8761 ggaataccac ctgccacaga tgcttcaaac ttgatgctaa cattatttaa gttagctaat
     8821 tctataaggt cactaccttt ttcagccagt aaatctttat tagctgtcac tacatgtatt
     8881 tttttattta acgctcttgt tacataatca aaagctggat ttaatcctcc aattacttca
     8941 actagtatat caatatcctt gtcaaatata tcttctatat cagtagttat cttatcaaag
     9001 tacttcttat acttgtgttt ttctttattt ttaactaaaa ttccaacaac ttctatatct
     9061 ctttttgaag tattattttc tattaaatca attaatccac ttcctactgt tccataccct
     9121 aatattgcta tcttcactgt aacatacccc catcattttg attatattaa attatttata
     9181 tatataaata taccaataaa aaaagcttga gtataaactc aagccatatg acgggtttat
     9241 actcatcttt cagcataaat gctgcaggat ttagcaccaa tgtataatta aatatactgg
     9301 ttgccgggct tcacagggcc agtccctctg ccactcttga taagattgct aatttaaatt
     9361 tatgtttaat agtataatac aatttcttcc aattgtcact atttttttaa cttatttccc
     9421 taaaaggata taaactatta tctaacagct cttttagttg gtttaccctt ttttgtaaca
     9481 ttattatttt tagtctttgc tttcgcttgt tcatgtcctg gaaaaagtat tttaggctct
     9541 tcaatattcc tcttgtaaat agtaaattta aaaccaatag cctgcacaaa ttcagctctt
     9601 aaagcttcac atactgcatt agcagtttct ttagtatcaa gacctgcatt ttctaatata
     9661 gtaactttta ctaattctct agttcttaac ataccatcta attgctctaa gaaactctct
     9721 gtaacacctt ctttaccaat ttgtgtagta ggctttaaag tatttgccaa agatcttaaa
     9781 tacgcccttt gttttccttt taacatattc tcacctctat aattctatac ttaaattatc
     9841 tataatctat atttaaatta attgtaaaac tcaaattcta attcataaat tttaaccaca
     9901 tctccatctt ctattcccat ttctctaagt ctatcaaaaa caccttgact ctccatagct
     9961 ttttggaaat attgaaggga ttccatatca tcaaaattta cagaatacat tattcttcta
    10021 agtgctttac ctgtaactac atacacacca tcttcaatat ctatacttaa ctcttcttca
    10081 gtaccaatat caagttctgg tctatacatt tcttcttctg aaactagctc cacatcttct
    10141 acttctttta gcattttaga aacataggct attacttcat ctatgccttg acgagttgct
    10201 gctgacattt taaatactgt atatcctctg ccttctaatt cactcttaaa cttctcaaac
    10261 ttactctcat cttctaatat atcaaattta tttgcaacta caacttgtgg tctctttgaa
    10321 agcttttcat tgtacaattt aagctcatca tttatcttgt caaaatcttc aatagggtct
    10381 cttccctcta atccagatat atctacaata tgtatcaata cctttgttct ctcaacatgt
    10441 cttaagaact catgacctaa tcctatgcct tcagaagcac cctctataat tccaggtata
    10501 tctgcaagta caaaactatc tccaaactta gtttgaacaa cacctagatt tggtgtaagt
    10561 gttgtaaaat gatagtttgc aattttgggt ttagctttag ttacaactga tagaaatgtt
    10621 gatttaccta catttggaaa tcctaaaaga ccaacatctg ctatcatttt aagttcaaga
    10681 gttatccatc tttcttctcc atctgtacca gattttgcaa aagctggagc ttgtcttact
    10741 gcatttgcaa agtgttggtt acctttacca cctataccac cttttgcaac tatagctgta
    10801 tctccttctt tttttaaatc tgctaataca agacctgtag cctcatctct tattactgta
    10861 cctgctggta cttttaatac taaattttct ccatttttac cagctctttt tcctttgctt
    10921 ccgtcgccac cattttgagc ttgatatttt ttctggtact taaagtccat tagagttcta
    10981 agccccaaat caacttcaaa tatgatgctt gctccacgac caccatctcc accgtctgga
    11041 ccaccagctg gaacgtattt ttctcttcta aatgctaccg aaccatttcc accatttcca
    11101 gccttaacaa atattcttgc cttatctata aacaaattct tcacctactt aattaaaaac
    11161 ttttaattag cattaccaaa aataccatta taaattcatc agtattataa gaaaaaggag
    11221 tgcctcaaca ctccttttat gtatattact cagctattgc tactggatat atactaactt
    11281 tttttctctt cttgtctttt ctttcgaatt taacagtacc atcaactaat gcaaataaag
    11341 tatcatcact accttttcca acattagttc ctggatgtat tttagttcct ctttgtctaa
    11401 ctattatact tccagcagtt actaattgac catcgaatct cttaacacct aatcttttag
    11461 atatagaatc tctaccattt ttagaagaac ctaccccttt tttagatgct aataattgta
    11521 aattcatgtt taacatatgg agtcaccccc tactttttta aaatttttat attttttggg
    11581 tattcccgtt taatttcttt aaccgctaat tgaaagtgat ttagtaataa ttgtgcgtct
    11641 tttacatgtg tatctttgac tatacactct atatgacctt cttctcctac tactttttca
    11701 aattcaatct tctgtaattt gtctaaagaa tttataactg caatagcatt agatgtaact
    11761 gcagcacata ctatatcata gcctatttca gcaaaatcag catgtccctt taaacaaaaa
    11821 ccttgcaaca agaaatcatc attataataa tatttaactt ttatcataac ttaaccaata
    11881 actaagcgtt tattttttct acaactatct tagtgtatga ttgacgatgt ccttgttttc
    11941 ttctgtaatc tttttttgct ttatacttga aaactataac tttcttagct ttaccttgtt
    12001 caacaacttt agcttgaaca gatgctcctt ctactactgg agaacctaat tttaattctc
    12061 catctttaga gcaagctaat acttcgttta aagtaacaac atcacctgcg ttagcttcta
    12121 atttttcaac gaataataca tcaccttcag aaactttata ttgctttcca ccagtcttta
    12181 ctatagcgta cattaaatac acctcctcgg cctcaaactc gccagtacaa ggtacttcat
    12241 ttgaagattt aaacctttac tgtgcggcat tacaattatt aattataaaa tatttacatt
    12301 ttattgtcaa ctattttatc gaaaataaca ttggttttat tgtgctttaa tgtactattt
    12361 gcgtttaaag aaatctttat attatatttt tcaccaattt tatcaattat atccatataa
    12421 tattctttta tttgttcaaa aataaaatca ttaaattcta ccattatgtt tttgtaaaca
    12481 gtatgttcac ttattctcat aatttctttt tcaatactat ctagtatata atgtacagat
    12541 tttattgttt gttcaccatc acaacatgga caagacatta aataatactt atcaatggat
    12601 tctttttctc tacgcctagc aacttctact aaaccaagtt ttgtcatacc taatacttca
    12661 gcttttcttt tatctttatt aaattcttct tctaggatgt gtaaaatctc cttttgatgc
    12721 ttctttttat gcatatcaat aaaatcgatt attattatcc cagctatgtc tctaattcta
    12781 agctgtctta caatttcttt tgcagcttca agattagttt tataaacggt ttcatcaagt
    12841 ttaccagttc caataaactt acccgtattt acatctataa ccgtaagagc ttctgttttt
    12901 tcaattatta aataacctcc actctttaac catacttttt tatttaataa tttttctatt
    12961 ttagattcta ttctgtataa atcaaataca tctctatttt cctcaagaca cagtttatca
    13021 acataagtct tatctatacc tcttaaaata gacttaagtt cagagtactt atcataacta
    13081 ttggtaatta tagattcaat atcatcattc acattatctt ttacatattt agttgcaaaa
    13141 tctaaagact tatataataa tttcggacct ataccaagtt tatattcttt taaaatgttt
    13201 tcatacttta atttcaactc ttctatatct ttctttattt catcatgagt gcaaccttga
    13261 gcctcagtcc ttattattaa tcctagattt tccttattta ctgccttagt aatttttttt
    13321 aatctaaatc gttctttttc atcagtaatt ttatttgata ttgtagttct atcattggaa
    13381 ggtatataga caatatacct tccagaaata ctaatctcag tgtttaattt tgcgccttta
    13441 gttcctattt cttctttatt tatctgaacc aaaatatcct gaccagattt tatagtactt
    13501 ccctttttta attgtaaata agcaagtttc ttaaatccaa tatctacaaa acaagcttct
    13561 attcctggta taactttttt tacgattcca cgatatatat tggatacagt cttcttattt
    13621 aaattgtctt caacaaaaag ttctgttaat ctttcatctt caagtactgc tgttttctgg
    13681 gaccctatta gggactcaat aactatcttt ttcacaactt cacctctcga gcttacatag
    13741 gagtcactaa ttccccatct tctaatacat ataaatctct tctcaatata tcaacatcta
    13801 atgggtcaat atcaagttca aacatctcta atatctttgg tataagtata ttagtattta
    13861 aattagtttt tgagcctgta gctatagtag cagtaagagt aatatgttca tcttctaaat
    13921 ttaaaacatc aaaggttctt atcataggtc ttatatctac ttcaaccatc ttaccttttt
    13981 tattcttttt tgttatgatt atttctttat tattcataaa gtcaattact tttcttttta
    14041 caaactcttt cgtcataggt cttttcaaat ctatattaaa caaatattct ccatagtcta
    14101 tcacagaaga aagagaaggt gtttgtctat caatttcttt tgctttgata aaatctatcc
    14161 catctggaag ttgaattgat actttattta aaaattcctc tactgataaa tcatcttctt
    14221 ctatttcaat atctacatac tcaccttgac tttctgtccc taaagccaaa gcattgccat
    14281 aactcatttt agggtgagga ttaaatcctt gagaatgtga taagtttatt tcagctcttc
    14341 taaacgctct ttgtaacaat ctctgaaggt caagatgtga tatatatatc atgtcgcctt
    14401 cctttttaaa ttttactctt attatcttac tcatattagc aaagccccct tcctatatca
    14461 tgtatattta ttccacaacc attgcagtta tgtctacaat cagaagtgat tttttctttt
    14521 ttagcatttt cgtcttctct aattaagaat tttttactaa tacctacatc tatatggtcc
    14581 caaggaaata cctcttcata gtttctattt ctatgtgcat aaaaatctat agaaagatta
    14641 ttcttttcca tagcttcttt ccatatatct aaattaaaat gttctgccca tccatcaaat
    14701 ttagcaccta gtctaaatgc atcatatata acttttccta ttcttctatc gcctctagct
    14761 actactgctt ccatcaaact agtctttgaa tcatgataat tgtattttat attgttattt
    14821 cttaatttat ttactaaatg tctctgctta ttaataactt cttctgtagt atcttgacca
    14881 tgccattgga aaggtgtaaa tggctttggt acaaatgtag aagtactcac tgtaacacta
    14941 aaacttctct ttagctttcc tccatttact tttctataaa tatcaataac tttataagct
    15001 aatttagcta taccatctaa atcttcataa gtctcagttg gtaaaccaat cataaaatac
    15061 aacttaacac tattccaccc catctcaaaa gctctttctg ttgcattttc caaatcttct
    15121 tctgtaacac ctttatttat aacatctcta agtctttgag ttcctgcttc tggagcaaaa
    15181 gtaagtccag attttcttac ttgttgtatt ttatcagcaa tttccattga aaaattatct
    15241 aatctaaggg atggaagcga aatcccaata ttattagatg catattcatc taccaaataa
    15301 tcagtcaact ctgataactt actataatca ctcgtactaa gtgatgataa tgatatttca
    15361 tcatatccag tattcttaac taatttatct aatatttctt taagtctttt aacactcttt
    15421 tctcttattg gtctgtatat catacctgct tgacagaatc tacatcctct agtacatcct
    15481 ctaaataatt ctagtactat tctatcatga acagtatcta tataaggtac tattagtttt
    15541 tctggataat ctacagtctc aacatctttt attatccttt tagtaggatt tttagtaatc
    15601 ccttctctat taggagttac actttgaact gtaccatctt cattgtactt gacatcataa
    15661 aaacttggta tgtatactcc ttctatgcta gatattttat ataaaaaatc ttctctagtc
    15721 gttttatttt tcttccattc tttatactca tttactactt ctaaattaac ctcttcacct
    15781 tcacctaata ttactacatc tacaaaatct gctaatggtt cggaattata agcacaagga
    15841 ccaccaacca ttataaatgg gtcttccaaa gttctttctt cttttaatat tggtatattg
    15901 gctaaatcta atatatttaa gatattagtg tatgaaagtt catattgaag tgtaaaagtg
    15961 acaaaatcaa agtttgttat aggctctctt gactctaatg caaataatgg tatacttttt
    16021 tctctcatta tattttccat atcaactgca ggagaataca ctctctcaca aaatacatct
    16081 tcatccttat ttattacatc atataatata tgactcccta agtgactcat accaacttca
    16141 tataaatctg gaaagcaatg tgcatatctt atcaattcac tattactagt atctttatga
    16201 attgaattta tttcatttcc aagatatcta gcaggctttt ctactttttt taagattttt
    16261 ttcaagtcta ctttattcat ttattaaaat tcctccaaac aagctcttta tttttattct
    16321 tatttataag taatattata cacgataaaa tattttctat caaaaatata actaaaatat
    16381 tgaatttaaa atacatatta ttcacaaaat agaatagcta aaaatattta agaatatttt
    16441 ctattaaatt tttattgaaa tacaattttt ttacaaagta tgatatatta taaacataaa
    16501 actattgttt tacatgactt atcatgacat cttttgtttc attcacctca aaatggggta
    16561 tgttacttat ataatgataa attttaggag ggttaaaaat gagtaaagtt tttgaagtaa
    16621 aaaaagatat ctactttact ggcgtagtag atgaaggttt aaaagtattc gacataataa
    16681 tggaaactga gtttgggaca acttacaact cttatttaat caaggacgaa aaaacggttt
    16741 tatttgacac tgtaaaggca aattttaaag atgagttttt aagtaattta tcagaggtta
    16801 ctgatatagc taaaattgac tatgttgtaa ttcatcacac agaaccagac catgcaggta
    16861 gtttaaaata cttattagat ataaatccta atatagaagt ttattgtact aaagctgcca
    16921 aactatactt agatggtcaa ataaatagac cttttaactg tcatgtaata aaagatggtg
    16981 aaattttaaa tatcggtaaa agaaatctta gatttataac tgctccattc ttacattggg
    17041 ttgatacaat gtttacttac attgaggagg acaagactct tttaacttgt gatgcatttg
    17101 gttgtcactt tgcaagtgtt gatgcagagg tagtgaatag tgaagattat ctaaaatcag
    17161 ctaaacacta ctatgattgt atagttaagc catttgctaa acatgtttta agtgctgtag
    17221 ataaggtggt aggactaaac atagaatttg atactatatt gacttctcat ggtccaatgc
    17281 ttactaaaga cccaatggct gctgttaaaa gatatgttga gtggtctaca gaagctgtta
    17341 atacaactaa tcaaaatcaa gtttctatat tctacttatc agcttatagt aatactttag
    17401 agatggctaa aaagataaaa gaaggacttg ataaagaagg tgcaaaggct gaattatatg
    17461 atttagaaga tatgacatta acagaaatgc acgatacatt agtagtgtca aaagtaatat
    17521 tactaggctc tccaactata aacaaaacta tggttaaacc tatgtgggat ttattctctg
    17581 ttatagaccc aatggctaac caaggcaaga tagcaggagt atttggttca tttggttgga
    17641 gtggtgaagg tataactatg gctgagactt tacttaaatc tatgtcattc aaaatgcctg
    17701 tagaatctct taagaaaaaa ttcttcccta gtgaagagac attaaaagag tgtatggcat
    17761 ttggtgcaga atttgcaaaa ttagttaaat agccattaat cacacataat attatttaaa
    17821 ataaatgatt ttatatttaa aaataaatta aaaaggagtt gtctcaaaat taatttttct
    17881 aatctaattt taagttcaac tcctttttta ttttctaaaa tgactgatat aatgataaat
    17941 gcaccctttg tgcgagggtg cattatttat aatatgttct atttgtacac tagtatattt
    18001 gtattaattc acatatctag cgtatgctac catccctctt ccagaattag atgccgtttc
    18061 accaatttcc actttaataa ttacattatt ttctttacac ttttttgata attctgctgc
    18121 agaaaggttc ttaccagtaa aatctaaaga aaatggctta tcatattcaa aaggatactc
    18181 atcaggtaca ccatctggag ctaatgcaac cataccatat ggtgtattca taaaccctat
    18241 tacagtaact gtatgctttg atgggtctaa atttgaattt ttgcttgtaa acttaattgt
    18301 atttattgat gagttgatta aactacacgg aactccctca tacatttcaa tttttggacg
    18361 tagattattt ccataaactt catcaagtat tgccccttgt tgatctgcta tcatagtgtg
    18421 attcccaggt ccaactggcg cagaaggctt agctggcgta ggtttcttaa cagttggcgt
    18481 tggattctgt gtctttacta cttcgccaac agctggatat gctttcttgt caaatggtaa
    18541 tgggtgagta agactcttgt atatgatgtc tgctcctgct gatttgttgg catcaatatc
    18601 tacatgatac aaacgtccat cctcaaaacg aataagattc cactgatggt catttgttgt
    18661 actatgtaca taataacatt caattcccat agcatcacat aaagctttca agctcctaca
    18721 cattccagaa caagcacctt ttttagtaac caatgtataa gcagtattgg ctccctctgc
    18781 tgcgttccag ttaacatatg ttgtgttgtt tttaatgtga ttatgtatag atacaatcca
    18841 ctatggagaa atataaaaga aaagagtata acaaacaata aaaaataaga tgattcaaat
    18901 tttattttga aacatcttat ttactctaca aattaagatt atttggtgaa ttatttaatt
    18961 attatttttt attaaagaaa ttttaaatct taatataaaa tacgctatta tacataataa
    19021 tccaaatgca aatgctactg gaattaacat ctccgattca aaataaaaga aatattctaa
    19081 gaaaatatct ggatttacta tattcccatt cttgtctaaa aaatcttttc cattatatcc
    19141 taaatgctgt ataaaattac ttatgttatt ataatctgct tccctctcat ataccatatt
    19201 agcattcaat cctgaagaac ttaaattagt gtttccatct ttcatatcta ttacatatag
    19261 tctaagagct ttgactttac tatacttatc tttatcaagt attttttcaa gttctaactt
    19321 atattcacca atctttgtca tatcatgtct ttctgccaat tgctttattt ctccatcaag
    19381 ttcttttcct aaattataca tattatgttg taaactatta ttgtaatacc acataaatcc
    19441 agaaattaaa aatgcaatta tacttatgta acctaatact tttttaaacc ccattacaat
    19501 tgatttatgc ctatccaatg ataaagatag ttcttttatt atattacata attcatactg
    19561 catttcatca ccatcatcaa atctttcaat tgctttttta catgcttctt cttcatctaa
    19621 tccttctaat ttaaattcat ttgcagattc tataagatgg catctcattt cctgttttaa
    19681 ttctatagta cttttattgt cacatttttt atatagttta tctaaatatt catctattag
    19741 tttcataaat taatccacct ctcccaaaaa ttgattcata acctttttaa taaatctcca
    19801 ttcttgtttc tttatttcta aaaaatcttt accttcatta gttaaattat aatattttct
    19861 tcttccacct gaactttgtt cattgctata ataagattta attaaatttt tagattccat
    19921 tcttttaagt gcaagataca ttgttccttc ttttaattca aactcacttc tttctcttac
    19981 ttgctttgca atttcatatc cataacaatc tttcttttct aatatagaaa caattaatat
    20041 atcgatataa ccttttaaca cttctttatt taactgcatt atgtaccacc gccttttcac
    20101 ctactactct ataatgtata gtacttatat ttactataat attacatagt actctataat
    20161 gcaaggttta tattctaaaa tagactcaaa aataataaat agctgtatat taatatttaa
    20221 tctataaata ctaatataca gctatttatc aaattttaaa aataaatttt tgttttatta
    20281 atgttgtcat aacaaca
//
LOCUS       DAKBNN010000054        20277 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000054 DAKBNN010000000
VERSION     DAKBNN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.054"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            103..3051
                     /locus_tag="OUB02_001679"
     CDS             103..3051
                     /locus_tag="OUB02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885440.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3029..3493
                     /locus_tag="OUB02_001680"
     CDS             3029..3493
                     /locus_tag="OUB02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8885441.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3606..3908
                     /locus_tag="OUB02_001681"
     CDS             3606..3908
                     /locus_tag="OUB02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885442.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3970..5334
                     /locus_tag="OUB02_001682"
     CDS             3970..5334
                     /locus_tag="OUB02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCT8885443.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5369..5842
                     /locus_tag="OUB02_001683"
     CDS             5369..5842
                     /locus_tag="OUB02_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885444.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            6014..6664
                     /locus_tag="OUB02_001684"
     CDS             6014..6664
                     /locus_tag="OUB02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCT8885445.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6838..7770
                     /locus_tag="OUB02_001685"
     CDS             6838..7770
                     /locus_tag="OUB02_001685"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCT8885446.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8044..8757
                     /gene="ispD"
                     /locus_tag="OUB02_001686"
     CDS             8044..8757
                     /gene="ispD"
                     /locus_tag="OUB02_001686"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCT8885447.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8754..9239
                     /locus_tag="OUB02_001687"
     CDS             8754..9239
                     /locus_tag="OUB02_001687"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCT8885448.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9334..9542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9606..11321
                     /locus_tag="OUB02_001688"
     CDS             9606..11321
                     /locus_tag="OUB02_001688"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCT8885449.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11443..12888
                     /locus_tag="OUB02_001689"
     CDS             11443..12888
                     /locus_tag="OUB02_001689"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCT8885450.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13326..14807
                     /locus_tag="OUB02_001690"
     CDS             13326..14807
                     /locus_tag="OUB02_001690"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCT8885451.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15028..15183
                     /locus_tag="OUB02_001691"
     CDS             15028..15183
                     /locus_tag="OUB02_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885452.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15328..16725
                     /locus_tag="OUB02_001692"
     CDS             15328..16725
                     /locus_tag="OUB02_001692"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCT8885453.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16710..17123
                     /locus_tag="OUB02_001693"
     CDS             16710..17123
                     /locus_tag="OUB02_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCT8885454.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17229..17987
                     /locus_tag="OUB02_001694"
     CDS             17229..17987
                     /locus_tag="OUB02_001694"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HCT8885455.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            18000..18737
                     /gene="rlmB"
                     /locus_tag="OUB02_001695"
     CDS             18000..18737
                     /gene="rlmB"
                     /locus_tag="OUB02_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCT8885456.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18740..19267
                     /locus_tag="OUB02_001696"
     CDS             18740..19267
                     /locus_tag="OUB02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCT8885457.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19332..19979
                     /gene="sigH"
                     /locus_tag="OUB02_001697"
     CDS             19332..19979
                     /gene="sigH"
                     /locus_tag="OUB02_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HCT8885458.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20053..>20277
                     /gene="tuf"
                     /locus_tag="OUB02_001698"
     CDS             20053..>20277
                     /gene="tuf"
                     /locus_tag="OUB02_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCT8885459.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPN"
BASE COUNT         8399 a         2103 c         3786 g         5989 t
ORIGIN      
        1 ttaatttaaa tgttattact ttataaaaaa ataataagta caaaaaaaac aatatatggc
       61 atataagttg ctatataaat aataaggagg gaacaattat aaatgaggca aagaataaaa
      121 aacttaattg aaaatgaaga taagaaaaat cctttaacag atgaagcgat ttcaagtagg
      181 ttaaatattc gaagagaaga tgttaccttt ttgagaaatg agttaaaaat agaagattca
      241 agacaaagaa gaaaagttgt tttaattaaa gctataaaag atattttaaa agaagaaaaa
      301 actataaata aaaatgatat aacaagaaga ttaaacagca ttgggtttac agtatcaaga
      361 tttacagtca tacaatactt aaaagagatt caagattctg gtgacctaaa acttgataaa
      421 gagagtgatg ataaaaaaga agaaataatc aataatagag aaaataaaga tatagcattt
      481 gataaactca taggtagtag tggtagctta agtacaatta taaaactagc caaagcagca
      541 attctttatc cacctcatgg tcttcatact attattatgg ggccaactgg tgttggaaag
      601 agtgagttag cagagtgtat gtataaattt gcagtagaat catccagatt ccctaagaat
      661 gcaccattta tagtttttaa tgcagcagat tattcagaga atccacagct tttgctatca
      721 cagttatttg gacacgttaa aggtgctttt acaggagctg atgagaacaa agaaggatta
      781 gtatcaaagg cagatggagg aatattgttt atagatgaaa tacatagatt acctcatgaa
      841 ggtcaagaaa tactatttca actgattgat aaaggtatgg tcagaaagtt aggagaaaca
      901 agactaacac ataaaataga tgtaatgatt atttcagcaa cttctgagcc aatagattct
      961 catttactta atacatttaa aagacgtata cctgtaacaa ttgaaatacc agaattagtt
     1021 gcaagaccat taaatgagag gtttgacatt ataaataatt tcttcttaat agaagctcaa
     1081 agaatgggag caaacataca tataaagtca gatgtattaa aagctcttat gctttacgat
     1141 tgtataggta atgtaggtca acttagaagt gatattcaag tagcttgtgc taggggacta
     1201 ttaaaccaac ttacaaacaa actaaaagaa gtaaatataa gcatatcaga tgtacctgga
     1261 tatgtaaaac aaggtcttat aaaaatcaga aattgtagag gaaaaataga aaattatgta
     1321 gatggggatt taattataga ctcaaatctt caaggtgaga tagaaaagaa aaatgaagat
     1381 atatatactt ttccagatga aatatacaaa cttactgaaa gaagacatat tgatttattg
     1441 gaacaaggtt tagattttga tgttataaat agaatcattg gaggagaatt agaaagttgc
     1501 attcaaaaat acataaaaca agttaaaaaa gtttctatta gtaacaatct tccagatata
     1561 agtccaattg ttggaaaata tatcgtagag ctaacttatg aagtaatcaa aatagcatca
     1621 caatacctaa gttcactaga accgaatcta catttatgtt tagcagttca cctaaaagca
     1681 gcttttgaga gattaaaaga aggaaaagta atatcaaatg ctcatttgaa agaaataata
     1741 ggaaattatc cactagaata ttctgtagca gaaaaggtag ctcaatattt aaaggaaaga
     1801 tataacataa acttaccaaa agaagaaata ggtataatag caatatattt taaaatgagt
     1861 agtagaagag atatgtctga acaaaaaaag ataggtgtac ttcttatggc tcacggaaga
     1921 gtagcatctt ctgtatgtga ggttacaaat aaactattag gaattaatca cgcaaggtat
     1981 ttagatatgc catttgagaa gaaaccggaa gaaatgattg aagaagctac tacaataata
     2041 aaagatattg atgaaggtag aggtgtgatt atattagtag atatgggttc actttcatat
     2101 tttggagata ttatttctgg aaagactgga attaatataa ggacaatatc tagatttgat
     2161 acattgcttt ctatagaggt tatacgaaga gcagttttac cagatttaac aatagacgag
     2221 atagttgatg aagttaaagg tatagagcca caagtaaaag aaaaggttag taaatgtaat
     2281 aatacaaaag tagattttag aaaacctata attttaacat tatgtattac tggattaggg
     2341 ggagcttgta agataaaaaa aatcctagag gaaaatatta aaagtataac tgaaaaggtt
     2401 gatatagaac ctataggtat gattgaatat ccaagtcttg atttagagat aaaacaaata
     2461 agcaatgata aaaatgtaat cgccatagtt ggaacaattg accctcagat agcaaatatc
     2521 ccttttttta gtctagtaga cataaatgat aaagcaaagt taaatcaaat aaataaccta
     2581 attgatacaa atcaaattgg tgaaacaaat aaaaaatatg aattatcaga tttaatacat
     2641 aaagaagata tatttgtgga tttacaggtt aaatcaaaag aagaagttct aaaatatatg
     2701 agtaaaaaac ttagagagaa aggttatgtg acaaaaaatt tctataaaaa agtagtggaa
     2761 agagataact tatttccaac tgaattatca gatgaaatag caatacctca cacagatgga
     2821 atagatgtaa ttaggccagc tataagtata gccatcttaa agaagcctat tgtgtggagt
     2881 aaaagtaaag taaaaataat acttttatta gctgtagacc aaaagtgttt taatccatta
     2941 agcactctac tctcatttat agaaacagat gaatttaaga agataaaaaa cattaaagat
     3001 agagatttta ttagggagat gatattagat ggagtcaatg aaaataatta gtagcaattt
     3061 aatttttaaa aatattgaag tgtctaacaa tgaagatgca ttaaagtttt tgggacaacg
     3121 tttgtttgac gaacagtatg taaaagaaag ctacatacaa gctgttgtag caagagaaaa
     3181 aaagtatgct acagggttac caacagaaat atacggagtg gcaataccac atacagatat
     3241 agtacatgta aatgagccag gaatagcaat tggaatactg aataaacctg taaaatttat
     3301 aatgatgggt acagacgata ctgaaattga tgtaaaagtt gtatttatgt tagcagtgaa
     3361 agagccacaa gaacaattgc aactattaga aaaacttatg actatatttc aagataagaa
     3421 tatactaaat aatattgttg acttaagtga agaaagtgtg agtgatttat taaactctaa
     3481 attgaaaaat taaacatgta aagtatatta aaaatgtaaa attaaacatg caaagtatgt
     3541 taaaaaatat aaaaagatta ttaaagattt ctaactatag atatctttaa aataaaataa
     3601 tttcaatggg ggtgactaat gtggctaaaa agatattagt tgcatgtgga acaggagtat
     3661 gtacttcaac aattgctata aacaaattaa agaaagcact tcaagacatt ggtaaattgg
     3721 atatggttaa tattacacaa tgtaaagtag tagaagttgc atcaaaagca cctgattatg
     3781 atttaataat ttgtactaca caagtatcta gtagtataaa aacaccagtg ataaatggat
     3841 tacctttttt aactggtgtc ggaatggata aattaatcaa tgatgtatta gaagagttag
     3901 aattataatt aaaaaaatta aggaacatgg tctctaaaat cataagtttt agagacttaa
     3961 aattaaataa tggagggtct ttatatggag atattaaatt tcattgttgg tctaggtgca
     4021 caagtaatga tgccaataat aatatgtatt tttggtttga tattaggtac aaaactagga
     4081 aaatctttaa gagctggttt aactgtggga gttgggttta taggtcttaa cactattata
     4141 gctttgctaa cagataattt aggtccagct acacaacaaa tggttaagaa tttagggcta
     4201 agtttatcaa taatagatgt aggatggcca gcagcatcag ctatagcttt tgcatcaaca
     4261 gtaggggctt taattattcc aataggtctt gtggtaaata tagttatgct aattaccaat
     4321 actactcaaa ctgttgatgt agacatatgg gattactggc attttgcttt tacaggagct
     4381 ttagtggcag gggctactca aagtgtaatg tggggagtat ttgcagcagt agcaaatatg
     4441 gtaattgtat tggtaatggc agatttaact gctccaggta tagaggaata tttaggtatg
     4501 ccaggtatat ctcttcctca tggatttaca caggcatttg ttccaattgc tatagtagta
     4561 aataaattat tagacttaat accaggcata aataagattg agataaatgc tgatacattg
     4621 cagaaaaaat ttggattatt tggagaacct ttaataatgg ggtctgtaat aggtgtaata
     4681 attggtattg ctgcgaaata tgatataaaa ggaatattac aattaggtgt aactatggga
     4741 gctgtattaa tattaattcc aaagatggca gcattattaa tggaaggatt attaccagta
     4801 tcagaagctg cacaggaatt tattgagaag agatttaaaa acagaggcaa gatttatata
     4861 ggcttagatt cagcagtagg aataggtcat cctgttacat tatcagttgc actagtgttg
     4921 gttcctttga ctatacttat agcagctata ttgccaggaa ataaagtttt accatttgca
     4981 gatttagcag taattccatt tgcacttgta ttaatcgtac caattacaaa gggaaatgtg
     5041 tttagaacat taataatagg aataattata attacttctg gattattaat cgcaactaac
     5101 ttagctccat tatttaccca aatggcttta aatgctagtt ttaaaatgcc tgaaggagca
     5161 actatgattt caagtatttg tgatggtgcg aatccattaa gttgggtatt tgtaaaagtt
     5221 atgaactata aagttattgg aggagtagtt tttggagtta tagctttagg aatggctatt
     5281 tacaatagga atagaataat aaatgaaaat aaaaagctat ctatagaaga ataaggttaa
     5341 aacaatttta ttaaggagtg tataggttat gaaaaaatat aaatatgatt ctaaaagata
     5401 cttactcagt gttacaatac caggattttt tatgatttta atattgattt atgcactgtt
     5461 taataactat acaaatttta atttgaatat atatagtttg ttaataattg tctgtactta
     5521 tggtatattc aatactttta tatcatcaag taatcctaaa aaaattatta ttgatagcaa
     5581 atgtatacat tttacctcat ttatgactac acataagtat gaaattaaag atatagaaaa
     5641 gataagaata agagaatttt ataataaaaa aatttatcta aaaattaatg atggaaaact
     5701 tttcaaaggt aggtattgga taagaacaaa tatgtttaat aatagtatag cattatatag
     5761 ctatttcatt gagttggaag agtgcttata tccagattca ctaaaattta gaaataaaaa
     5821 atttgagaat aagaacatat aaaaataaaa aattgaattg aagcctagct taatcttttt
     5881 tgataagata taagtttgaa ctttatacta aatttttaga attttcaatc aagacaataa
     5941 cataatagct aagcaattag aacacaacta agatgtataa ggcatgtata tgatgaatgg
     6001 gaggatttaa ttaatgttat tagagaaatt aagaaaagaa gtattacaag catctttaga
     6061 ccttttaaac tataatttag tgacattaac aggaggaaat gtaagtggga gagatgaaca
     6121 aactggctat atagcaatca ccccaagtgg aatggattat agaaatctta caccaagtga
     6181 tattgttata gtagatgtag atggaaatat tatagatgga aaatggaaag catcagtgga
     6241 tttatcagac cacttgtata tatataagca tagagaagac ataaatagta taattcatac
     6301 acattctaca tactcaagtt gttttgctat tttaaatgaa cctatagaat gtgcaagtac
     6361 aactcttgca aatgaagtag ggggttcagt accagttgca aaattttcgc caccaacatc
     6421 taaaaagatg ggaaaatgtg ttattgaagc aattggtgac aaaagagcat gtcttttagc
     6481 aaatcatggc gtaattgcag ttggtccatc tgtaggacat gctcttacag ccgctgtaat
     6541 gttagaagat tcagcaaaag tatattactt agcaaaatca attggcacac cagtgctact
     6601 gcctgatgaa gaaattcaaa gagcgagaga tgtatttttt aatgtctatg gtcaggataa
     6661 ataaattaga taaaatttta aaagtaatat gaaaatatag atattcaatg atattaagga
     6721 gatttataat gatataataa gtttaatagt taagaagtaa aatatgaaat gtaatagtat
     6781 ataaattcat ttaagactaa aaagaataag ataaaataaa aaggagtctg tatttctatg
     6841 tattacttat taaacaaaag cacaaatcct tactttaatt tggcattaga agaatatcta
     6901 tttttaaatg ataaatataa tgatgatatt ataatcatat ggagaaatga agaatccatt
     6961 tttataggta aaaatcaaaa tccatatcag gaagtatatc atgatgttat tgaaaaggga
     7021 gaaattccta ttttaagaag aatatctgga ggaggaactg tttatcatga cttaggtaat
     7081 atcaatatgt cattcataca aaaagatagg caacttcatg agatagattt tttggagcat
     7141 actaagttta tgcaaaagat gctttcaacc ttaggactag atgtatctat aactgaaaga
     7201 aaagatttat ttttaaatgg gaaaaaaatt tcaggctctg cacagtctat aaaaagaaaa
     7261 aattctcttt atcatggaac tcttctttac gattcagatt taaataaatt aactaaatat
     7321 ttaaattcaa ataaagcaac tgaatcaaat gcaactaaat ctgtttcaag taaagttaca
     7381 aatattaagt ttttactcga aaaagatata aattactttt tagattattg tgttgaatat
     7441 ttaaaatcta atataaagaa tataaaagaa cttgaattgg atgaagaaga tattaaaagt
     7501 gtatacaata taggagaaga taaatataaa aagatagagt ggacatatgg taaaacgccg
     7561 aaatttcaag tagtgctacc atttaataat gaaagtaaaa taaatataca tgttaacaga
     7621 tggaaagtat caaaattttt tatttcttat gatgataatg taattaatat ggacaagttg
     7681 ttagatttga atttctttaa agaagaaata aaagagagta ttttagaaaa ttatccacat
     7741 tacatagatt taatcaattt aatattttaa tataaaacct ctaatttgat aaaattgtct
     7801 tatatcaaac tagaggtttt taatattctt gcatattaac aaccattaca aaattgtaaa
     7861 attaaaaaca acactattga aaaatcaata tatattgtaa taaatatatg tgtttttcat
     7921 tagcaaatag acattaacta tttatacaac tgaaaattta atatataatt aatcagatgg
     7981 ttaaaataaa aatattaaaa gttttaataa atgatggtta aaaataaaat acgaggtgat
     8041 accatgtata gtgttataat tgttgcagcg ggaagcggaa ggagaatgaa cttagatata
     8101 aacaaacagt tcataaaatt aagagaaaaa gaaattattg ctcatacaat tcaagttttt
     8161 tatgaaaata taaatataga tgaaatagtg gtatgtataa aaaaagaaga agaagatttt
     8221 tttaaagaaa atataataaa taagtataac tttaaaaata taaaaatagc atatggcgga
     8281 aaagaaagac aggactcaat atataatggt ttaaaaaaat tagataaaaa ttgtgatata
     8341 gtacttatcc atgatggagc aagaccattt gtcgaccata gaataatcaa tgaatctata
     8401 aaggtagcta aagaaaaaaa ggctgtggtt gtaggtgttc cagtaagtga tactataaag
     8461 atagtgtcag atggaacagt acaagaaact ccagaaagaa atttgctgtg ggcagcacaa
     8521 acccctcaaa cctttgaata taatctcata atagatgctt atgaacaagc atataagaac
     8581 aattattatg gtactgatga ttcaatgttg gtagaaaata taggtcaaag tgtcactatg
     8641 gtaatgggtt cttatgaaaa cataaaaata actagtccag aagatttaaa tatagcagaa
     8701 caaatattaa atatggaaaa aagagatgaa gttaacagta gaaggagaat tatatgagaa
     8761 taggattagg atatgatgta cacaaattaa cagaggatag aaaacttata atcggtggtg
     8821 tagagatacc acacgataaa ggtttgttgg gtcattctga tgctgatgta ttgatacatg
     8881 caataatgga ttctatttta ggtgctctag cattaggaga tatagggaag catttcccag
     8941 atacagatga agaatataag ggagcagata gtatgaaatt attagaacat gtgtataatc
     9001 taataacaag taaaggatat aaaattggta atatagacag tactataatt gctcaaagcc
     9061 ctaagatggc tccatatatt gagagtatga gaagtaatat atcaaaagtt ttaaacacag
     9121 acattgataa tataaacata aaggcaacaa cagaagaagg tcttggtttt acaggagcaa
     9181 aacagggaat agcttcacaa agtatttgtt tattattgtt gacaagtcag aataattaat
     9241 atattattag taataagttt aatggatatc aagtaatagg cctatataga atatctataa
     9301 tgtatgaatt taagtgaaat taatataaat ataaaattag ctatgataaa gaaatagtaa
     9361 aatgatgaaa tcttcacaga gagaaaacgg tggtgagagt tttcaagagg aagtctttga
     9421 acctgtcttt tagcttaaag gtataaaaat ctttacggta ggaaccgtta tattcccaaa
     9481 agagagctat tcatatagct aactagagtg gtaccgcgga aattaaacct ttcgtctcta
     9541 tacttgtata gagatgagag gttttttata ttttcaggaa gttaatattc aggaggaatc
     9601 tattaatgaa aatgtcaaaa atgtttatgc cgacattaaa agaaatccca gcagatgctg
     9661 agattacaag ccatcaatta atggttaggt caggcatgat aaaaaaaatg acttcaggtg
     9721 tttataacca attaccaatg gggttaagag tgttcaaaaa aattgaacaa ataattagag
     9781 aagaattaaa taaaaaagat tgtcaagaaa tactttgtgc agccttactt ccttctgaat
     9841 tatggaaaga gtctggaagg tggactgcta tgggtgaaga aatgtttaga ctaaaagaca
     9901 gaacagaaag agagtactgt cttgggccaa cacatgaaga agcattcact gatataataa
     9961 gacaagaaat tacttcatat aaacaattgc cactgaactt gtaccaaata caagtaaaat
    10021 atagagacga gagaagacca agatttggtg ttatgagaac taagactttt acaatgaagg
    10081 atgcctatag ttttgatgta gatgataaag gtttagataa gtcatatcaa gatatgtttg
    10141 atgcatatgt aagtatattt gatagatgtg gattggagaa tagccctgta caggcagatt
    10201 caggagctat aggaggttct acatcagccg aatttatggt taagtcagaa gttggtgaag
    10261 atgaagttgt attttgtagt ggatgtgatt atgcagccaa tgtagaaaga gcagaatctt
    10321 gtaatctagc atcacaaaaa gaagaaatga aagaattaga agaggttcat acaccaggag
    10381 cagctacaat aaaagagtta gaagaatttt taaaaacttc tccagataag tttgctaaaa
    10441 ctttagtata tgaggcagat ggaaaaacag ttgtagtcgt tgtaagagga gatagagaag
    10501 ttaatgaaat caaagtatct aatgccatag gttctgttat agaatttgct cttgctacag
    10561 atgatgtagt gagaaaagta actaatgcag aagtaggatt tgcaggtcca ataggtataa
    10621 atgctgacta tgtatttata gataaagaaa ttgttgaaca aagaaacata gttgttgggg
    10681 ctaataaaac tgagtaccat ataaaaaatg ctaactatgg aagagatttt gaaggaatag
    10741 ttggagactt tagaaatgtt caagaaggcg acaagtgtat agtatgtggt aagccacttg
    10801 aaatagctag aggtgttgaa gtaggacata tatttaaact aggaactaag tactcagaat
    10861 ctatgaatgc aaacttcata gacaaagatg gaaaatcaaa gcctatagta atgggatgtt
    10921 atggaatagg tgttgaaaga acagcagcag ctataattga acaacataat gatgagaaag
    10981 gtataatatg gcctttgtca gtagctccat accatgttgt gatagttcca gcaaatatga
    11041 aaaatgaaga acaaatatca atagctgaaa atatatataa tgatttgcaa gctatgggtg
    11101 tagaagtttt attagatgac agagatgaaa gaataggtgt caaattcaat gattcagaat
    11161 taataggaat acctatgagg attacagtag gtaaaaatat taatgaaggg aaagtagaat
    11221 ttaaacttag acataaagaa gataaagaga taattgacat agaagaaata aatgaaaaag
    11281 taaaggcaga gtttatcagg aataatgtta ggttaggata ataacttaaa taagatattg
    11341 tatagataat caaagttgta ctttatagac accattttat ctatatataa ggatataaaa
    11401 tggtgttttt ttataaaaca aataaatgag gaggtaattg aaatggcaaa aaatgaaaaa
    11461 caatttgttg aagaaataac aaagatggaa gatgattttc cacaatggta cacagatgta
    11521 ataactaaga ctgacttagt tgattatgca ccagtaaaag gttttatggt tgtaaagcca
    11581 tatggatatg cattatggga aaagatgcaa gaatttatgg ataaaaaatt taaagaaact
    11641 ggacataaaa actgttattt tccattatta ataccagaga gtttattaaa caaagaagca
    11701 gaacacgttg aaggttttgc tccagaagtt gcatgggtaa cacatggagg aaataaaaaa
    11761 ttagaagaaa gattatgtgt aagacctact tcagagacta taatatgtac aatgtatgcg
    11821 aaatggttaa aatcatatag agaattacca tatctttata atcaatggtg ctcagttgtt
    11881 agatgggaaa aatcaactag acctttctta agaacatctg agtttttatg gcaagaaggt
    11941 catacgttac atgaaacagc agaagaagca caagaagaaa caatacaaca attagaagta
    12001 tataaagcgc tatgtgaaga attacttgca atgccagttg tagctggtca aaaaagtgaa
    12061 agtgagaaat ttgcaggtgg tgaaagaaca tatactatag aagctatgat gcatgatggt
    12121 aaagcattac aatcaggtac aagtcacttc ctaggtcaac actttactaa ggcttttgat
    12181 attacttttg cagataggga aggaaattta gctaatccat accatacatc ttggggagca
    12241 tctacaagac ttataggtgg acttataatg actcatagtg ataacagagg tttagtatta
    12301 ccaccaagag tagctccaat tcaagttgtt atagttccta tagctgctaa aaaaggaaat
    12361 gtaatggaga ctgttgataa aatatatgct gatttaaaag ctaagggagt agctgtagaa
    12421 gttgatgata gagataacta tacaactgga tggaaattta atgagtggga aatgaaaggt
    12481 gttccagtaa gagttgaaat tggaccaaaa gatatagaaa ataatcaagc tatggtattt
    12541 agaagagata ctttagaaaa ggattctatg cctttagaag gattagctga tgctatatgt
    12601 gatttatttg atgttataca caatgatatg tttgaaaaag caagaaaaca tagagaagac
    12661 aatacatcta tagttgaaaa tatggatgag tttagaaaag ctttagaaga aaagccagga
    12721 ttcataaaga ctatgtggtg tggagatgca gaatgtgaag ctaagattaa ggaagaaaca
    12781 ggagcaacta taagatgctt accttttgaa caagaaaact taggtcacaa gtgtgtgtac
    12841 tgtggaaaag aagcagatag tatggtagta atggcaaaag cttactaaaa tacatttaaa
    12901 taaaaactgg ggtagaaagt atttaatact ttatcccagt tttttgtttt ttatgagtaa
    12961 taaaatgtat ttgtaactat gtcataagat aagtcataga tacttatatt aaaaagaata
    13021 taatagagaa ctatttttaa taattacata ttaatgaaag caaaatattt tgttatatag
    13081 attttcaata aagcaaacaa taaatataaa tgaatattag taaggagtgc ttttaatttt
    13141 tagaaaataa atgacttaaa agaaagattt atgaaattaa aactaggatt ttctgatata
    13201 ataaacatgt gtatttttaa ataaaaagga tattattatt agatttgatt aaaaataaac
    13261 ttgcaaaaca atgtactagt ttaatattat agttatattg aataaaatat caggaggaag
    13321 taaaaatgaa tgtaagagta aggtttgcac caagtccaac aggatttgta catataggta
    13381 gtttgagaac tgctttatat aactatttat ttgcaaaaaa aatgggcgga gaatatatat
    13441 taagagtgga agatacagac caaagtagat tagtagaagg tgctatagag aatatgctta
    13501 atgctatgaa atgggctggt gtcaaccata gtgaaggtgt tatattagat gatagtggaa
    13561 aagtagtaca aaaaggagaa tatggtccat atattcaatc tcaaagatta gatatatacc
    13621 aagaatatat aaagcaactt ttagatagtg gaaaagctta ctactgtttc tgtacaaaag
    13681 aaagactgga tgaagtaaga gatgctcaaa gagcagcagg agaaacagct aaatatgatg
    13741 gtcattgtaa gaatctttct aaagaagaag tagaagcgaa tataaaagca ggaataccat
    13801 atgttataag attaagattg ccagagaatc atacaattaa gtttacagac ttagttagag
    13861 gagatatgga atttaatact aatgacttag atgaccaagt attaatgaaa acagatggat
    13921 ttccaactta tcattttgca gtagttgttg atgactattt gatgaaaatt acacatgtta
    13981 taagaggaga agaatgggta tcatctactc caaaacatgt ttatttatat gaagcttttg
    14041 gatgggaagc acctgtattt gtgcatttac caaatatact taataaagaa aagaaaaaat
    14101 taagtaaaag acaaggtgat gtagctgttg aagactttaa gaaaaaagga tatttaccag
    14161 agggattagt aaactatgta gcattagttg gttggtctcc tgaagataat caagaattgt
    14221 ttacaatgga ggaattagaa aaagcgttct ctgtagaaag agtatctaaa agtggtggag
    14281 tatttgatac tgaaaaatta aattgggtta atcagcacta tataaaagat ggtgatgatg
    14341 cttatctaac agacttagca ataccatttt taatagaaga tggatttata acagaagaag
    14401 aagctactaa taaatatgat ttcttaaaga gtatgatatc agtacttaaa gagaaattac
    14461 aatatgtaaa agaagttact gaacatgcaa gtatattttt tggagataaa atagaggtag
    14521 aaactgaaga gggaagagag ttcttaagat tagaacatat acctacttta atagatgctt
    14581 tgagagaaaa aatagaaaaa acagaggtat taaatgctga gtttgttcaa gctatgctta
    14641 aagaaataca aaaagagtac aaaataaaag ggaaaaattt atttatgggc tcaagaataa
    14701 ttttgacagg gcaaatgcat ggtccagatt taccaaaagt aatggaagtt ttaggaaaag
    14761 aaacttgctt aaatagaata gcttatgtaa aaaataatat attatagtca taaaagtccg
    14821 ctaatttaga agcggacttt ttttgtgtta gtatttggtg aaaatatctg cttaagaaaa
    14881 tgaaggtatt taaggtaata aatttaatat ataaaaagca aagcaaaaat ataaataaaa
    14941 tatgaataat aaactaataa aatattaagc atagaagaag ggaatctatt attttagttt
    15001 gatttaatta ttaagggggt aattactatg tatagtaata ttgatgatgt taaaaaggaa
    15061 ttaaaggagc tatgcttaga atatgttaca attttagaaa aattaaaaga tgaaaaaatg
    15121 attacagaag aaacttttga aaagtgctct tctcaaaaaa aaatattttt agaagaacaa
    15181 tagaggatgg taatagtatt attattatgt agtttgaaag atagtacgaa aaatgatagt
    15241 aatactatta tttattgcaa ttttcaacta taatataata tataatgtta agaagataat
    15301 ttataaaaat acagtgaggt gatgagtgtg aaagtttata atacattgac tagaacaaaa
    15361 gaagaatttg ttccattaga agaaggaaag gtaaaaatgt atgtgtgtgg tccaacagtt
    15421 tataactata tacacatagg taatgcaaga ccatttataa tatttgatac tctaagaagg
    15481 tatttagagt atagaggata tgatgtaact tatgtacaaa actttacaga tgttgatgat
    15541 aagattataa atagaagtca tgaggaggga atatctcctg aagaagttgc ggctaagtat
    15601 ataaaagaat attttgtaga ctgtgatggg ctaggaataa aaagagctac tgttcatcct
    15661 caggttacag ataatataca acagattata gagtttataa aagagttgga agataaggga
    15721 tatgcatatg cagtaaatgg tgatgtatat tttgatacaa ataagtttga aggttatgga
    15781 aaactttcag gtcaaaaaca agaagattta gaggcagggg ctagaattga ggttaatgac
    15841 caaaaaagac atcctatgga ctttgttctt tggaaggcaa aaaaagaagg tgagccagga
    15901 tgggatagcc cttggggaga aggtagacca ggatggcata tagaatgttc tgttatgtct
    15961 aagagatatt taggtgaaac aattgatata catgcaggtg ggcaagattt aacattccca
    16021 catcatgaaa atgaaatagc tcaaagtgaa gctagaagtg gaaaaacatt ctcaaaatac
    16081 tggatgcata atggatatat aaatataaat gatgaaaaga tgagtaaatc aaaaggtaat
    16141 ttctttacag taagagatat atcaaaatta tatgatttag agatagtaag atttttcatg
    16201 ttatcagcac attatagaaa tccagtaaac tttagtgatg agatgttaaa tcaagctaaa
    16261 gcaggtcttg aaagactata taatactaaa gaaaaattag aatttacgct aagtaattta
    16321 gtagaatctc cgttaacaga aaaagaagta gagcttgtta aagaattaga tgactttaga
    16381 caaaaattca tagatgctat ggatgatgat gttaatacag cagacgcagt aagtgtaata
    16441 tttgaattag ctaagttaat aaattctaat gtagatgaaa attcttcttt ggagtttgct
    16501 aaaaaatgct tagatgaatt taatgaactt acaggagtat taaatattgt aaataagaaa
    16561 aaggatactg tattagataa agacattgaa gaattgatac aaaaaagaac tgatgcaaag
    16621 aaaaataaag agtttcaatt agctgatgat attagacaac aattactaga tatgggaatt
    16681 gtattagaag acacaagaca aggtgttaag tggaaaagga tataaaaatg gaaaaaactg
    16741 aattagttac tatgtcacca ttagttttag catacttagg tgatacagtg tatgagacat
    16801 atataagaga atatttaatt agacagaata cacaaagaaa agttaatgat ttgcataaac
    16861 tagctataaa gtatgtaaaa gctaaagctc aggcaactat aattcatgaa attgaaattg
    16921 agttaactga agaagagagt aaaatttata aaaggggaag aaatcaaaag tcaaatactt
    16981 ctcctaagaa tgcagatatt atagactaca aacatgcaac tgggtttgag gcattagttg
    17041 gatatttgta tttaaataat gaaatagaga gacttcaata tattatcaat aaaggaatca
    17101 aaattattga aagagatatg taaatattaa agagtgttgt cttaataata agtaataagg
    17161 caacactttt tttataacta atgtataata aatatataga aattagtaat gagaaaggat
    17221 aaaaagttat gaaagtaaaa ttaatatcac atacacctga accagaaaaa gtaatagcta
    17281 tggcagctaa gctatgttat tcaccagttg ggacagacga aatagaaaaa gacttaacag
    17341 atgaaagtat agagaaattt ttaaatatgt tattaagtat aggtcatggg tcaatattag
    17401 aacatgcttc atttacattt tcaatagaag gtatttcaag ggcatgttca catcaaattg
    17461 ttagacatcg tatagctagt ttttcacaac aaagtcaaag atatgttaaa ttagaacaat
    17521 ttgagtacat aattcctcca gaaatagaaa aaatagaaaa ggcaaaagag ttatttatag
    17581 actccatgaa aaaagaccaa gagaattatg ataagttagt tgaaatattg tttgaaaatc
    17641 attataatga cctaataaaa aacggtaaaa atgagaaaac agctaaaaga caagctgaga
    17701 agaaagcaat agaagatgct agatacgtat ttccaaatgc atgtgaaaca aaaatggtat
    17761 ttactataaa tgctagaagt ttgtttaatt tctttgaaca tagatgctgt gaaagagcac
    17821 agtgggaaat aagaaattta gctgtggaga tgttaagaga ggttaaaaag gttgctccta
    17881 tattatttaa aaagactggt ccaagttgtg taaatggaag ttgtccagaa ggaactatga
    17941 cttgtggaga tatagttcaa gttagagaaa aatttaaggc cttgtaggag gtgaataatt
    18001 tggcgagtat agaaggaaga aatccggtaa tagaagcaat aaaaagcgac agagaaatag
    18061 ataaaatatt aattgcaaat tcagctaaag aaggttcaat taagaaaata ataggaatgg
    18121 ctaaagataa gaatataatt attcagtatg ttgataaaca taagctagat gaagtaagta
    18181 caagtcattc acatcaaggt gtaatagctt atgcaagtga gtataagtat tatgaattag
    18241 atgagctaat agatttagca aagaataaag atgaagaccc atttttcatt atattagatg
    18301 agataacaga cccacataat ttgggttcaa taataaggac agcagatgca gtaggagcac
    18361 atggagttat cattccaaaa agaaggtctg tacacataac tcctgtagtt gccaaagcat
    18421 ctgctggagc agtagaatat atgccagttt gcaaggtaac caatatagtt aatacaataa
    18481 aaagactcaa agaagaaggg ctgtggattg ctgcagctga tatggatgga gaaacttttt
    18541 ataaacaaaa tcttactggt cctcttggtg tagtaattgg aagtgaaggt tttggaatat
    18601 ctagattggt taaacaaaac tgtgacttta tagtaaaaat gccaatgata ggtaatgtta
    18661 catctttaaa tgcatctgta gcaggtggaa tacttttata tgagatattc agacaaagac
    18721 tggataaaag taaataggca tgaaaaaaaa tataaatcat tacttaataa tagatggcta
    18781 caatattata aatgcgtggg acaatttgaa agaactggct aaagaggact tagaagattc
    18841 tcgagaaaag cttattgatg acattataga attttctgag tttatgggtt ataagactat
    18901 aatagttttt gatgcttata atgttaaaaa ttctcgagaa aaagttgaaa aaagaaaaca
    18961 cataacaata gtttatacaa gagaacacca aacagcagat agttatatag agaaatttat
    19021 aacttcttta tcaaagtatg atgatgtaaa agtagccact aatgactatg cagaacagca
    19081 aatgatttta ggaaaaggtg ctactagaat gtctgcaaga gaattaaagt tagagttaga
    19141 tcgttccaag aataaaatga aggaaaaaaa tatcggtttg aggaaaaaaa ttcagcgaaa
    19201 ttggttggag gaaaggttag ataaagaaac attgtcgaaa cttgagaaca ttcgtagaaa
    19261 gcgttgaaat tactaggttc tttagactat aatataccta taaatttgtt ttatatatgg
    19321 gggagattta catgttggta gcaaaagaaa aaagttatga gttagtggac aattgtcagc
    19381 aggatgagta taacattgta ttaagagcaa gtgaagggga taaaatagca ctagagtaca
    19441 ttattactaa atacagaaat tttgtaaagg caaaagcaaa gtcatatttt ttaattggag
    19501 cagataaaga agatataata caagaaggaa tgataggcct ttataaggca gttagagatt
    19561 ttgatggaag taagaccaat tcatttaaat gttttgcgga gatatgtata acaagacaaa
    19621 taataactgc tataaaaact gcaacaagac aaaaacatat acctttaaat tcatatgttt
    19681 ctttgaataa accaatatat gatgaagagt cagacagaac acttttagat attatagcta
    19741 caagtatagt aacagatcca gaagagctta taattagtaa agaagagtta aaaaatatag
    19801 aatcaaaaat gaacgaattg ttaagtgact tggaattaga agtactagaa ttatatttaa
    19861 atggaaagtc ataccagttt atagcggaca agcttaaaag agatgtaaag tcaatagata
    19921 acgctttaca aagggttaag agaaaattag aaaaacatct tgaaaacaga aatgattaat
    19981 agtaaaatac ttaccataag cttattgtga gcaaaattaa tttatatcga aagataaggg
    20041 aggaaattca aaatggctaa agctaaatac gaaagaacaa aacctcatgt taatataggg
    20101 acaataggac acgtagacca cggtaaaact acattaacag cagcaataac aaaaacatta
    20161 tatgacagat atcaattagg agaagcagta gatttcgcaa acatagataa agctccagaa
    20221 gaaagagaaa gaggaatcac aatatcaaca gcacacgttg agtatgaaac accaaat
//
LOCUS       DAKBNN010000055        19981 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000055 DAKBNN010000000
VERSION     DAKBNN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.055"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..625)
                     /locus_tag="OUB02_001699"
     CDS             complement(<1..625)
                     /locus_tag="OUB02_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885460.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLITI"
     gene            complement(612..1379)
                     /locus_tag="OUB02_001700"
     CDS             complement(612..1379)
                     /locus_tag="OUB02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885461.1"
                     /translation="MEEILYVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            1667..2341
                     /locus_tag="OUB02_001701"
     CDS             1667..2341
                     /locus_tag="OUB02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885462.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2365..3369
                     /locus_tag="OUB02_001702"
     CDS             2365..3369
                     /locus_tag="OUB02_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885463.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            3481..4710
                     /locus_tag="OUB02_001703"
     CDS             3481..4710
                     /locus_tag="OUB02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCT8885464.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            4718..5392
                     /locus_tag="OUB02_001704"
     CDS             4718..5392
                     /locus_tag="OUB02_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885465.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5395..6627
                     /locus_tag="OUB02_001705"
     CDS             5395..6627
                     /locus_tag="OUB02_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885466.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(6913..8178)
                     /locus_tag="OUB02_001706"
     CDS             complement(6913..8178)
                     /locus_tag="OUB02_001706"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCT8885467.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASKLGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8231..9379)
                     /locus_tag="OUB02_001707"
     CDS             complement(8231..9379)
                     /locus_tag="OUB02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HCT8885468.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVSLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(9479..10024)
                     /locus_tag="OUB02_001708"
     CDS             complement(9479..10024)
                     /locus_tag="OUB02_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HCT8885469.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10247..11191)
                     /locus_tag="OUB02_001709"
     CDS             complement(10247..11191)
                     /locus_tag="OUB02_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCT8885470.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            11577..12458
                     /locus_tag="OUB02_001710"
     CDS             11577..12458
                     /locus_tag="OUB02_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HCT8885471.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(12519..13727)
                     /locus_tag="OUB02_001711"
     CDS             complement(12519..13727)
                     /locus_tag="OUB02_001711"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCT8885472.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(13800..14052)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14204..15193)
                     /locus_tag="OUB02_001712"
     CDS             complement(14204..15193)
                     /locus_tag="OUB02_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HCT8885473.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            15345..15785
                     /locus_tag="OUB02_001713"
     CDS             15345..15785
                     /locus_tag="OUB02_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8885474.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            16112..16339
                     /locus_tag="OUB02_001714"
     CDS             16112..16339
                     /locus_tag="OUB02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCT8885475.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            16365..18485
                     /gene="feoB"
                     /locus_tag="OUB02_001715"
     CDS             16365..18485
                     /gene="feoB"
                     /locus_tag="OUB02_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCT8885476.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(18618..19436)
                     /locus_tag="OUB02_001716"
     CDS             complement(18618..19436)
                     /locus_tag="OUB02_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8885477.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            19643..19927
                     /locus_tag="OUB02_001717"
     CDS             19643..19927
                     /locus_tag="OUB02_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885478.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
BASE COUNT         7102 a         2990 c         2555 g         7334 t
ORIGIN      
        1 aaatagttat taataaaaca ccaagtacac attttaactt tataaatttt ttaaacttat
       61 acttttctgg atatttatta aataactcta taattctctt atttctgata actatagtat
      121 ttcttattcc aataactata aatattatta aaaatacagc taatgtctga actaaggcct
      181 ttatacttaa gttcatattt actactatca tttcattcat taattttaca agcatcatag
      241 tcataagctt tgaaaataac aatcctaaaa atatacctat taatattgca actataccca
      301 tcaaaaaagt ttcatataaa aatatttttc ctatctgatt tttcctcatt ccaagtaaac
      361 tataagttcc tacttctcgc ctacgtctat tgaaaaaaaa tgagtttgaa taatatataa
      421 ataaaaatac aaataccact attacaattg aacttgtatt aatactcatc ctagttttct
      481 ctccaagtga actcaacgct tcattatact ctatagattt aaaactaaaa aatacaaata
      541 cactaaatac aattgaaata aagtacatta aataattaaa aaaactattc tttacatttt
      601 gcattgcaat tctaaataaa gtcattttta ttaactcctc caattaagga aactgtattt
      661 actattttat tatagaactc tctgttactt tctcccttta caatttctgt gaatattatt
      721 ccatccttta taaagagtat tctattgcaa aaactcgcac ttatagcatc atgagttacc
      781 atcaatattg ttttattgct gtctttgttt aattttaata gacaattcaa caaatcccta
      841 gcagatttag aatctaatgc tcctgtaggc tcatctgcaa gtactaatga tggttttgtt
      901 gctatagctc tacatacagc tcctctttgc ttttgtccac cagaaacttc gccagggtat
      961 ttatctaata tagattctat atttaattct tttgaaatct cttttattct attttctatt
     1021 actgatactc tttcattttt taaagaaaga ggcaacatta tattttcttt tattgtaagt
     1081 gtatccaaaa gattagaatc ttgaaataca aagcctagat tatcacgtct aaaatctgcc
     1141 aattcatttt gcttcatttt aatagtattc tttccattta tataaatatc tcctgatgaa
     1201 ggtaaatcta ttgtagaaat aatatttaaa agtgttgatt taccagcacc ggaagacccc
     1261 ataataccaa caaattctcc tttataaacc ttgaaagtta ttcctcttag agcttcatgt
     1321 tttgaacctt ttcttccata ctcctttttt atattttcaa catataatat ttcttccata
     1381 gttaataatc acctttcatc acaattagtt atttatcaat tctcacaaat atattatata
     1441 tattaaaatg gcttttaaac atttaattat attaatttaa ggttacattt tcgtaagtat
     1501 taattatatt tcttcaaatt tattgcaatt atgtttaaaa tataatttat caagtaaaat
     1561 atagttatat taatataatt attttaggta ataaggtaga aatattttaa tttatcaagt
     1621 agaatatagt tatgttaata taactatttt gaattaggag gtaagaatgt ttaaaataat
     1681 ggtagttgaa gatgacgtct cattgaaaaa tatcatagca aagtgtctta ctaaatgggg
     1741 acatgatgtt catcaaatag aaaatcttga aaatataata gaagagttca aaaactataa
     1801 cccagaatta gttttattgg acataaacct tccattttat gatggttttc attggtgtaa
     1861 tgaaataaga aaaatttcaa aagttcctat aatttttatt tcttcaagaa attctaatat
     1921 ggatgtaata atgggtgtaa atttaggtgc agatgattat atacaaaaac ctttttctgt
     1981 agatgtctta gtagctaaag ttaatgccct tttaagaaga acttataatt ttgttgataa
     2041 taactcaaat cagattattc acaatggtgt tactttagat ttatcaactg caacaataaa
     2101 ctatgaagat aatactattg agctaacaaa aaatgaaatc aaaattctcc atgagcttat
     2161 gaaatataag gggcagatag taagtaggaa taaactcatg aaaaaattat gggataatga
     2221 ttggtttgta gatgataata cattaactgt caatgtaaat agaatacgct caaaattaaa
     2281 tgaaattggt ttagaagatt ttatagaaac aaaaagagga ttgggttata taatcagtta
     2341 aaacaattct aagtaggtga atttatgagc tttaaagatt ttctcaagga taaactaggt
     2401 tttttagtat acaattttac tttcttaatt tttacagtat cagttattat attttctcct
     2461 gtagatgttt ttctgactga tactatttta tatatattag ttgtaaatgt tgtgttttta
     2521 tttttatatc tatttattag ctacatcaag aagaacaaat ttttagataa gataaaaaat
     2581 gatacattca aaattaatat gaatgatata gaattagcta gatgtaaaaa tgaagaaaaa
     2641 atatacttga acattattaa aaatcatcaa tatcaatgca ataacataat aaataataat
     2701 atcgagaaat ttgaagaaaa caaagagatt ttaaatatgt gggtacatga tgtaaagata
     2761 cctatagcta taataaaact cattcttgaa caaaatgaaa atttaattga tgaaaaaatt
     2821 tcagatgaca tagatagtga aatatttaga attgaaaact ccgttgaaaa aattttatat
     2881 ttatcaagac tcgaagattt tcataaggat tttttaattc aagaggtaaa tattgaaaaa
     2941 atcattagaa atattataaa aaaatactca aaatatttta tttcaaaaaa aattatttta
     3001 gacttaaata atttgaattt taaggtttta tctgatgaaa aatggctata cttcatattc
     3061 gagcaattat tgagtaattc cctaaaatac tcctcattag gagataatat atctatctat
     3121 tgcaaaagaa ccaaaaatta tttacaatta agagtggaag atactggttg tggtataaaa
     3181 aaggaagatt taaatcgaat attcaataag ggatttacag gtaataatgg aagaaacaat
     3241 tctaaatcta caggtcttgg gctttattta gtaaaagaac tttgtgataa gcttggtcat
     3301 aaaattgatg tagattcaga gtacaaccaa tatacaaaat ttactattac attcaaatgt
     3361 gatttatagt atcttattat tgatagttta ttttcattaa cttatattca acttcttata
     3421 gtaattctat atgtacatcc cagtatataa ttaagtagtt tttataaaaa ggaggaagac
     3481 atgtcttttc taaaaaaatt taaaaataaa agatttagaa ataacagttt taaaagtaac
     3541 aactttagaa atagcagttt tggaagtagt ggcttcaagt ctaaaaataa aggttttaaa
     3601 aacaatccct ttaaaaataa gagattcaaa atatatgcag gaatagcagt aatcatactt
     3661 ttaattctag gagttcttgc ttatgttgat tcgaaaaaca ctgaattaca agctaatgaa
     3721 aattttatcg aaacatatac tattcctgaa aatgaaaaaa tatttataaa tgggatggtt
     3781 gttcctaagc aaactaagga cttcaatata tctggagatt atgagttaag tgatgttaat
     3841 gttacaaatg gtcaaaaagt aaatcaagga gaccttcttt ttactgctaa aaatccaact
     3901 ataatagcag aaatagatag tttaaaatca caacttagtc aatacaaaaa acaaaaaata
     3961 tcactttctg atattgctga aaataaagat gctattgctt ctataaatgc acagataact
     4021 gctttaaact ctcagatagc ttctctggat aaaaaagctt atgacagagt tactgctcct
     4081 tttgatggca cagtatactt gaatgaccaa actggaaatc cagaccaacc agtttcattt
     4141 atgactattc aaggtctaga gttttatatg aaaggacaag caagtgaaca agatttacct
     4201 aaaatgaaga ttgaccaaat ggtcaatata ctcgtttttt caactgacca aaagcttact
     4261 ggtagaataa gttttatatc tgataaacca tctactccaa atactgaaat gggagctcag
     4321 cagaatacac tatcttatta tgatataaac atagcatttg ataatcaaga aggattagta
     4381 aatggattcc atcttcaagc atcacttgaa gtagctaatt caagctttaa aataccagca
     4441 tcttgtgtac ttaaagacaa caaacactca tatgtattta aggatttaga tggtatattg
     4501 aaaaagcaaa ttgtagatgt agcaagtcaa aatgatgatt ttgctgtagt tagaggcggt
     4561 cttgaacaag gcgatataat aataaaacac cctactaaag aaatgaaaga aggagaccca
     4621 gttcaaggcg atggcgtaac tgctggttct aataatggag atactactcc aaacaaaaaa
     4681 gtagaagaag ttactatgga tgtgaattaa tttacatatg ttaattaaat tagaaaatat
     4741 tcaaaaatat tacaaagtag ggaaagatga attacatgtt ttaaaatcct taaatttaga
     4801 aattgaatct ggagaatttg tcatgataat gggtaagtct ggtagtggta aaactacttt
     4861 acttaatatc ttaggttttc tagatgtatt tgatgaagga agatatatat ttgatggaac
     4921 agatgtaact aatttgagtg aaaatgaacg ttctgttttt agaaatatta atattggatt
     4981 tgttttccaa caatttaatt taatagaaac tctaaatgta tatcaaaatg tagagttgcc
     5041 tctaatttac aataaagcac taaaaaaatc taatagagaa gaaattgtta aagataaatt
     5101 aagttctgtt gggcttttgg acaagcttaa gcagaaacca cttcaattat ctggtggtca
     5161 acaacagcgt gtagccattg cacgttgcct agcaaatgac cctcagataa tctttgctga
     5221 cgaaccgact ggggcattag atagtgaaac tagcagagaa attatggagc ttttaacaag
     5281 gctcaacaaa caaggtaaga ctataataat ggttacacat gatcaagatt taactaagta
     5341 tgcaactaag gttatacgac ttaaagatgg tgtgtttact tcggaggtgt aatcatgagt
     5401 ttattaaaaa attctttggc caatttaaaa ggtcacaagc ttcgtgtatt tgtagcttta
     5461 ctctggatta taataggaat aacttcagta atacttgtta gttctatagg taatggattt
     5521 caaaaagaaa ttaagaagtc tgtaaacaat gtaaacccta ataaaactac tatttcgttt
     5581 gaatcagccg ataacacagg tcttactgat gatatgagta tttttttaaa accctttaat
     5641 gcaaaagatt tagaagaatt atcatttgtt gaaggagtag aaagaatagc tccttcaaga
     5701 gatgggttta accttgattc agtttactct tcacaagcat cttttgataa aaaaactacg
     5761 tatgttgatg ttggaccagt aaaaaaagat tctaaaataa atttaatttg tggtagagat
     5821 ttttctttag atgatgaaaa aagaaaagta attttactta cattacaaag tacaagtgaa
     5881 atttttgaaa atccagaaga tgccttaggt catggtatca acataaatgg aactatattt
     5941 gaaataatag gggtattaga tgattctcag caaaaccaag ctggaggatt ctttggaggt
     6001 taccaagata tgcaatttac tacttcccta gtacctaaaa aggcttttga cactttaatg
     6061 agtcaaaatt cttattcaaa tgaaatttac cagttagact tagtttcttc taaaggctat
     6121 aatgtaaatg aagttgcaaa caatgttata gctaaattat atgagatgca ccctggtatc
     6181 aatggttctt atactacacc agacccaact gaacagacag cttatcttga aagcattaat
     6241 tccaatgtaa acaaatatgt atctataata actgtagttg caatgtttgt tggtggtatt
     6301 ggtgtaatga atataatgta tgtatctgtt atggaaagac aaagagaaat tggtataaga
     6361 cgtgctattg gagcaaaacc taggtctata ttatttcaat ttttagttga agctgttttt
     6421 ataacagtat gtggtggaat tttaggaact atagttggat ttactgctac taactatgtt
     6481 tctaaatata taggttttga agctatacct agcttaaata gcttatttta tgcaattgta
     6541 gctactatat tgactggagt tgtatttggt ctgattccag cctttaaagc ctctaaacta
     6601 gaccctataa aggcaattta taagtagaaa ttgtataaag attttcgatt tttagattag
     6661 tttatataaa acatacttaa atatataacg cactattata tatgatagat taggaactta
     6721 atctttccaa atataatagt gcgttctttc ataaaaagga ttctcttcct tactatatct
     6781 aaattaccaa aatgttatgt tctaattgtt caaactaata cttccaattt tgattaatca
     6841 atctcattct gctaatatac aatcttttac tttcaatcat tgtacactcc gccttaaaaa
     6901 aataatctta aattaggcgt tatatacaat ctccccatta catattgtat aattaacctt
     6961 tccatataaa gatttacctt taaacggtga attatcagct ttagaaacaa agctttcaac
     7021 tgtccattct tcttctggat tgaatataac aatgtctgct acagcccctt cttctataaa
     7081 tccaatattt aaattgtaca actttgcagg atttatagac attttttcta ttaactgcat
     7141 catactaaga tgatttttat gcaccaaatt tgttattcca agagctaaag aagtttcaag
     7201 cccaataatt ccacttggag cttttataaa ctctctatct ttttcttctt tactatgagg
     7261 tgcatggtct gttgctatta tttcaatagt accatcattt aatccctcta taatcttatc
     7321 tctgtcccat ttacttctta gaggtggatt catttttgca tttgttccaa actccaatac
     7381 atcttcctca gtcaatgtaa agtgatgtgg tgaagcttct gcaactacat ttgcacctaa
     7441 gtattttgca aatctaacca tatcaacaga tacacctgaa cttatatgtt gtatatcaac
     7501 tttagcacca gtctttagtg ccaacataca atctctggct accataacat cttctgctac
     7561 acttgatgca ccttttaatc ctagttttga ggctaccttt cctgcattta cacctggatt
     7621 tccaactaaa gaagggtctt cttcatgaaa actaagtggt acatccaact cttttgcctt
     7681 aatcattgcc tccataatta gattgctatc cattaaaggt aatccatcat cagtaaaccc
     7741 tggtacacca gcttttttaa gagcttccat atcaactaac tctttacctt caaaaccttt
     7801 agttatagct gctgcttgta aaacatttat acatgcattt tttgactttt ctttaacata
     7861 attaaaagta tcttcattat ctacaatagg attagtattt gccatacata taacagttgt
     7921 aaatccacct ctagcagcag atttagctcc actttctata tcttctttat atgtaaaccc
     7981 agggtctcta aagtgtacat gtacatccac taaacctgga gcaactacat taccactagc
     8041 atctattatt ttttcatatt catcactttc atcaaacttg cctattttat aaattttatt
     8101 ttcttttata attaagtcaa tattctcatc tctttgactt ttagggtcta ttactctacc
     8161 atttttaatt aaaatcatat tatttctcct ttatattttt aatttatata gactcatata
     8221 atattataca ttattttaca tacttaaata caaaattacg tttgacatca atagcctttt
     8281 taggacaaag ttcatggcaa cagtaacatc ttatacattt acttaagtct ataatagcac
     8341 ccttagtccc tatctctata gtggatgcag gacaagcttc cttgcatttt ccacatttta
     8401 tgcaatcttg aaatcttacc acaggctttg gtgttataag ctttgaaata ggttcattta
     8461 aaaattttgg tagctttacg gaaaacaatc ttaaatcttt acttacattt ggaatcttgt
     8521 aattttttat aattaaatct tttatatctt cacctaaaat ttcaatatct gaaaaatctt
     8581 cttgtatata tcctctctta atagcacctc taattgtagc aactctattt ggaagtaaac
     8641 ttataatctt acatgcaaca acatctatag catatggatt ttgtgatgct aatgaaactc
     8701 ctatttctcg agtagtacca gctgaaggtc catcaccttc catacctact ataccatcca
     8761 taattgtcaa tgttggtttt atataatcac aaatatctaa aagtacattt tcgcaaaaca
     8821 actcttctgt aggaaatcta taatgtatct ctgctttttt caatccagct atgcatccat
     8881 ataagttttt tgtaccccca gtaaaagttg ccattatatg agtctttaat ttacaaagat
     8941 ttattatata atctgaatct actattggtt taataatatc catatatttt agaacatgtg
     9001 ctttttcact atgtatctta acatctgaaa catcataatt taacttaatg cccagttcat
     9061 ttgctacttc ttcaattcca gatgccttat aaatgctctt aagagaactt tgattatatg
     9121 gaccaccagg gctatctgct attgtaatat cacaatttaa ctctaaaagt ttttcacata
     9181 caacttttac aaccatagga tgtgtagttg tagcttcttc aggctttttt ttcatcaata
     9241 aatttggttt tataagtact ttgcttcctt ttttaacaaa cttatctata ccaccaatat
     9301 cctctaaatt tttttcaata gataacttta cttcttcata attgtaagtt ttacaactcc
     9361 taacagatac cgacttcata ttatctccat ccttctatca caacagttta tttatataat
     9421 ttaaaaaagg agaaaccaaa gtttctcctt ttaatctatg ttttaattgt tttaattatt
     9481 attttccaaa atgtctgttt aataatccta agaatgcttt accatgtcta gcttcgtctt
     9541 tacacatttc atgtactgta tcatgtatag catctaatcc taattcttta gctctcttag
     9601 ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact cttaagtttt
     9661 cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca gcatgttcag
     9721 cctcttcaaa agctattctc ttatacgctt cagctacttc tggataacct tctctatcag
     9781 cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccagta aagttagctc
     9841 ttaatccttc gattatttct gcatctacac cttgagctac tcctattcta tgttcatcag
     9901 cccaaaccat ttcgcctttc atttcttcaa atttatcagc tccaacttta catactggac
     9961 attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa acaaattttt
    10021 tcataattaa tttcctccca attttatata attaaatttt tatttttaag tttaataact
    10081 tgctacaaga atatatatac cccagacttt atagcttaaa cactaaaaat aaaatttatt
    10141 tcctaattaa ttttatcact ttgaaacacc agtatcaacc aagttttgat gtcaaatttc
    10201 tacataatac ataatttatt tgattaatta ggaaatagtt gtcatattaa atactttcta
    10261 atgaagactc ctctgaatct ttaagtttca cttttttgct ctttaaaaaa gataatttag
    10321 tttctgcagt tatgattgca acaaatatca agatactacc aataaccaat tttgctgtaa
    10381 taagctcctt taaaattatt atagaaaata ttgtaccaaa tacagcctct gtggataata
    10441 ttatagctgt tctagttcca tcaactcttt tttgacatat agtctgaaat aaaaatccaa
    10501 ttgtggtact aaatacagct agatacatag ttcccatgta accagataat tcagcttcca
    10561 tctttaattg acctgcaaat gtttgaacta caacagacat taaaaacgct actgtaaatt
    10621 gaatagctgt caatgccatt gggttgttat cttttgcaaa ctcacttgta aagaatatat
    10681 gaaatgcaaa tccgaatgaa catataagtg ttagaaaatc tccaaaatta atagagaaat
    10741 cagcttctaa agaaagtatc ccaattccta taagagctac caaactactt attattccta
    10801 ttttatcaag ttttcttcta taaagtataa atcctatgaa cggaactatt acaacattag
    10861 ctgctgttat aaacgcattt ttagatggtg tagtgtacat aagtccaata gtttgtactg
    10921 tgaatgctag aaataaaaat attcctaaaa tccctcctgc ttttaataat tttttaccca
    10981 tattggcacg aatctgttta aaaaacagta aattcataat tatagctgcc aagaaaaatc
    11041 ttaaagttat gatttgtaga ggtgtaagcc caccgtctaa tgcaaattgg gttgctacaa
    11101 agccacttcc ccatataatt gctataaata ttagtcctat ttctccaaag tattttttca
    11161 ataaagcccc ctccttttta tttttttgca aaattataaa ccccctaaac agcaagagag
    11221 tttttctata agcattacta taaattaata tagtaaattt tttcagtatt ttaaatctaa
    11281 atttacatac attttgagct caaatttata tccactattt aacagtaata tattaatcat
    11341 tttttgtaaa caccttttta ataaattttt atatttttta tattttttta cctatactaa
    11401 atttttttat agatattttt aatatatatg atactttaat attatgcaaa taatgtcata
    11461 taagttacac tttagttatc ttagttacaa ctttgtcggt ttactttttc tactttcttc
    11521 gatataatta taatatattt ttgatacaat tcgacaaaag ggggaatgct gtaattatgg
    11581 taaaaaaaag aaaaaagaaa actgttacac ttactatact tgttgtaata gttattggta
    11641 ttttttctat ggcatgggcg gacatgtcta gaaaaaaaga aatgaaagaa actcaagaaa
    11701 gacttaaagc tgaaagatta gaaaaagaaa gaattgaaaa agaaaaaaat gaacctatta
    11761 ttggtgcaaa aaaagaagct gtaaaagaat atggatatga cgcaaaaata gtttgggaca
    11821 aacttaacaa atacgactat tcaaacaatg gagaaaagat agtctttctt acatttgatg
    11881 atggaccatc tactactaat actccacaag ttctagatat cttgaaaaga catggtgtta
    11941 gaggaacttt ctttattaag ggtgattctc ttgaaagaaa aggagctaat gaaattttaa
    12001 aaagaacatt tgacgaagga aatgcaatag cccatcactc ttatactcat gactataaaa
    12061 aactatatcc taacagaagc ctaaatctcg atacattcgt aaatgaactt aacaaaactg
    12121 atgaagctat gaaaaaagtc ttaggaaaga atttttcctc aaatgtagtt agatgcccag
    12181 gcgggtatat gtcatggaaa aatatggaac ctctaggaaa ctacttaaaa gagaaaaaca
    12241 tggcatcaat tgattggaac gctttaaatg ctgatgcaga aggtaaaaag aaaaatgcac
    12301 aagaattatt tgaacatgca aagaaatctt ctgaaggcaa agaaatggtt gttcttttaa
    12361 tgcatgatac ttatggaaaa caagaaacag taaatgcatt ggaccaaatt ataacatatt
    12421 ttaaagacaa tggttatcaa tttaaaattt taatataata aaaaagcaag tttattgaat
    12481 gtacatttta tactcaatag acttgctttt actttactct attttactag ctcgattttt
    12541 acaaattttt tctttcctat ttgaacaact aattcactct cttttacaat agtgcttaaa
    12601 tcctctactt tttcaccatt tacctttact ccaccttgag aagctaatct tctaacttca
    12661 ctcttactct taacaatctc attagaaact agtacttcga ttaagtcaaa tccatcttcc
    12721 ttaacttgaa ttgtttgtat atcttcaggt atttgacctt tttggaatac agatttaaat
    12781 ctttcttcag cttctttagc agattcttct ccatggtata atgttactat ttctcttgct
    12841 aagttcatct ttatatctct tggatttact gaaccctctt tcaactgaga ttctatttta
    12901 ttaacttcat caggatgaac atctgtcact aaattgtagt atttaattat aagttcatca
    12961 ggaatttcca tagatttttg gtacattatt cccgcttctt catctattcc aatatagtta
    13021 cctaaactct tactcatttt ttctttacca tctaatcctt ctattaaagg catcattatt
    13081 actatttgag actccatacc aaattctctt tgtaatgatc ttcccattag taaattgaat
    13141 ctttggtcag ttccaccaag ttctatatca gcttctaatg ctatagagtc atatgcttgc
    13201 attaatgggt agaagaactc atgtactgat attggcattt gtccttcata tctcttttta
    13261 aaatcttctc tttctaacat tctcgcaaca gtaatagttg ctgctaattt tattacatct
    13321 tcaaaattta acttagctaa ccactcgcta ttaaatctta caatagtttt ttctttatct
    13381 aaaactttga aaatttgttc ttcataagtt tttgcatttg caagaacttg ctctgttgtt
    13441 aaagcttttc tagctttaga ttttccagtt gggtctccaa tttttcctgt aaaatctcca
    13501 attattatta ctatttgatg acctaaatct tgtaattgtt tcatctttct aagaactact
    13561 gtatgcccta aatgtatatc tggagcactt gggtctagac ctaattttac tatcataggc
    13621 tttccttctt tttctgattt aataagcttt tcccttaact ctttctcatc tattaaatca
    13681 tctacacctt tcataattat tcgcatttgc tcgtctatcg atttcatttt tcattcctcc
    13741 taaaatattt atataatcta taatttttat ttttataata aaaaaagctt tcatcctctt
    13801 aaaaggacga aagcattgct tacgtgttac caccttagtt catcaatatc tcacgatatt
    13861 aatctcttag agtacaatta catatactcg tgtactataa cgtgtactaa tccgatatct
    13921 cttaatggtt gttatctttc agagatagtg ctctgagatg tattcaaact aaatcaattt
    13981 attccatttc accaaactgg aactctctgt taaataaaat ctagccctac ttcttctctt
    14041 catagcattt atatattcag attatttcat caattacaag aaatttttct taacttactc
    14101 ataatatatt attattttta tattttgtca acctttttta ttattatata catgtttttt
    14161 tacattatac tactttttta tattttttac caatacccaa aatttatttt tcaatcaaaa
    14221 gatttatttg tttattgact atattactct gactcaatct tatatttatt ttcttattta
    14281 aaaatgcgtt tataattatt aaactaataa ctgttacatc aaatataaaa aacagcaata
    14341 aaattattat caaaaataaa attctagcat ataataattt tctaaactta gctattataa
    14401 agttttgtag tgacatacat gagtctctaa tgataaaata gacacttata taggttattg
    14461 atataaagat tgtttttaaa taaacagaat caactttaac attcaacagt tttagaactt
    14521 caaaaatggc tagataagaa acacatatgt aaaaagcttt ttttgagcaa cataaaaatt
    14581 ggtttaaatt tctaaaattc ttaaatatta aaaatttttt taaagaatta gaatctaaaa
    14641 tatgcaattt attccctaaa attaaactta ataaatatat gcaaaacacc ataccaaaag
    14701 gaaacaatgc tatcattaaa taataaataa aatttgtata cccattaaaa taaatccatt
    14761 tgctatatcc ttgaaattct ttcataaaac tataacctat taatgttgaa gcatatatat
    14821 caatgaaaac cattacaata accccaaata ctgaaccata tactaaatca tccataacca
    14881 ttgtaggctt atttattaga tagccacaca ttataccaag taatacactc tgacttgccc
    14941 aaatagcagt tcctagattt ccaagtacca gagaaattat taaaagcgaa gtaattccag
    15001 ataaaatggt atactttaaa tcacatttca agcagattat acaaaaaaat actggcacta
    15061 taaaatctaa gtaaatagca tatccaaatc cagtactcac agctataatt gttactacta
    15121 tgaataaaga tgacaataaa gctgcttcag ttaatttttt agtagtattc aaaacactgc
    15181 ctccataaat cataaatttt ctaactaaag ttttgttttt aatatagtat atcacttttt
    15241 aaaaatattt ttaataaata taaattttaa taaaggtatt tatgttaaaa atggtataat
    15301 gtatcttaat attcagtttt ttaagatgct aggaggtaat tactatgttt aaaattaaac
    15361 actttaatga tttgagttta gatgaatttt atgaaatagc aaaatcaaga tatgaggtat
    15421 ttgcttgtga acagaagata ttttcattaa acgattatga tgatatagat aaaagctctt
    15481 atcacatatt tttaaaagaa aatggattga tttgtgctta cgctagaata attcctaaag
    15541 aatacagctc ttataatgat gtttccattg gtagagtttt agttctttct agtcatagaa
    15601 gaaaaggatt agcaaaacaa atgatggatt gtgctataga ttttataaaa gttaacttac
    15661 acgaaaataa tataacctta tcagcacaaa cttatataaa gaacctttac ttatcttgtg
    15721 gttttaaaga aatctcagaa gtatacgacg aagcaggtat tgaacatatt aaaatgagat
    15781 tatagtgttg acaagctaat attgttaata tataatttaa gtataaatta aatattgaat
    15841 tgatatttag gtatttgaaa ataccttaca tagagtcaga aggcttttgc tttctgactt
    15901 ttttattatc aaaattcaat aattatatgg attcaaagtg ttaattttta ttctatattc
    15961 tactttcccc aaaagtaaga attataccca actttgtatg ataggcatct tgcctatctt
    16021 ttttttgtaa aagtaacaag gtaataatat aatgcatagt ttaaaataag aagattagcc
    16081 caggctaaat ttcaagcatg gaggtgctat tatgaaaaac cttaacgata ttcaagtgaa
    16141 aaaaactgtc aaagttgaag atatattatc tagtggtaat ttaagagaaa gaatgttagc
    16201 gcttggtctt actagaggag ctgttataga tgtagttaga aaaggtccaa aaaataattt
    16261 aactgtctat aaaatcagag gtagcaagat agctctacga caagaagaaa gtagcttaat
    16321 attagtttct gacatttaaa aatttctagc aggaggatat taatatgggg ttgacacata
    16381 actcaactaa aatgagttct ttaaaagaca tgtttgatat agacaataag gaagaccagt
    16441 ttgtaattgc attggctggc aatccaaata caggtaaaag tactgtattt aatcatctta
    16501 cagggcttag acagcatact ggaaactggc caggaaaaac tgtagctaca gctcgtggta
    16561 attttaaata taaaaatacc gaatatgcat taatagattt accaggaaca tattctttat
    16621 ttgccttatc acaagaagaa attgtagctc gtgattttat atgttttggt aatccagatg
    16681 cagtaatagt agtatgtgat gctacatgtc ttgaaagaaa tttaaattta gtatttcaag
    16741 tgatggaact tacagataaa gttattttgt gtattaattt aatagatgaa gctagaaaaa
    16801 aaggcattac tattgataaa aaacttttag aggattctct tggtattcca gttattttaa
    16861 ctgctgcaag aaatggctct ggtatggatg aactattgga tactttaaat gatgtatctt
    16921 ttgacaaata caaattaaat aataaacctg ttagatacaa tgaaaatata gagaatgtgg
    16981 taaaatcaat tcaaccagaa ttggataata ttataccagg tataaattct agatggttgg
    17041 gacttagact tattgatggc gatgaaagta tatttgaatc tatgagtaat tatatagaca
    17101 aagattctat agatgctatt aatgaagtaa agaaaaagat tccagacaat ataaataaac
    17161 agaaaataag agatgagttt actaaaatca actatgatta tgctaaaaaa ctgagtgatg
    17221 agtgttgttc taatgttgct aaaaaatcaa ctgatagaga agaaaaagta gataaaatat
    17281 taacatctaa aatatttggg cttccaataa tgctcttatt attaggtact atattgtgga
    17341 ttactataga aggagctaac tatccatcca ctttattatc taatttatta ctcggatttg
    17401 agccaagtat ttcgggtata ttaaatagta ttaattgtcc atcatggtta aatgatatgt
    17461 tggttttagg attatacaga acattagcat gggtaatctc tgtaatgcta cctcctatgg
    17521 caatattttt tccattattt accttacttg aggattttgg atatttgcca agagttgcat
    17581 ttaacttaga ccacctattt aaaaaagctt gtgcacatgg aaaacagtgt ttgactatgt
    17641 gtatgggatt tggatgtaat gcagctggag ttattggttg taggataatt gactctccaa
    17701 gggaaagact tatagctata cttacaaaca actttgttcc ttgtaatggt cgttttccta
    17761 ctttgatagc tatatctaca atatttttta gctcagttat aaccaactct ttcgtatcaa
    17821 gtgtagcaac tgcactttgc ataactttac tgataattct aggtgttata ataacattgt
    17881 tggtttctta tactctttct aaaacgctat taaaaggagt tccgtctaca tttacattag
    17941 aacttcctcc ttatagagtt cctcaaatag gaagaacttt atatacctct ataatagata
    18001 gaacaatatt tgttttagga agggctgtaa tggtagccat acctgctggt gtcataactt
    18061 ggatatttgc aaatatatat ataggagatt taagtatatt atcacatgtt gcaaactttt
    18121 tagacccttt agctaaatta attggtcttg atggttttat attacttgct tttattcttg
    18181 gattcccagc aaatgagata gttgtaccta tacttttaat ggcttaccta gcaactggtt
    18241 ctatgataga attagatagc tttagtgctt taggtcaagt tcttagagaa catggatgga
    18301 cttatttgac agctttaaat gttatgctgt tcagtttact gcattggcct tgtgcaacaa
    18361 cacttttaac aataaaaaaa gaaactggta gtttaaaatg gactgcctta ggattcttaa
    18421 tgcctactat acttgctttt gttgtttgtt ttttaacaac tacagtttac aatttattta
    18481 tataattagt attaactgac attagacata tattagagaa actatttaaa aatttatata
    18541 ctttattata aagcatagag atatattcaa tgataagtaa aattacttaa catttgaata
    18601 tatctctatt ttaattctta tctggatact tggattgaaa tttcagtcat ataattatct
    18661 tcattacttt cacaaaaaaa gtcaagtata ctctcctcat acgaatttcc aataatttta
    18721 tattcatttt catatatata ttttttcaat ttttcgtaag atttatatga atcctcatat
    18781 tttccaatat gttttatgca agcatacatt ccttctggct ttacatatag ctttttatcc
    18841 tttatttttt tatctatctt caaataaaaa cttgatatac ttaaatagtc cccatttttt
    18901 atattttcat tacttactat tactcccaac tcatatcctt gatacaaatt tttcgattta
    18961 caataattgg tgaaatctat taattccatc ataaaatcac tattactcat attactcaaa
    19021 tcgcttgata gaactaaata ttcttcaaac tttttggata tataaatttc ttcactattc
    19081 attttagaaa ttccttcaac tgttatattt attctatttt gcatcttaga acttatcttt
    19141 cttagttttt ttattttttc tcttatcttt ttttcttctt ctttaagcat tttcacacaa
    19201 agctggggag ttctattttg tatatactct tttatttcct ctaaactcat tcctatttct
    19261 ctcaatgatt gtaatatatc aagtgtatca tattgataaa ctgagtaata tctataatta
    19321 tttttatctt tatattcagg agaaaaaata cctattttgt catagaaaat taatgtctgc
    19381 ttagatatac cacacaattt agcaaattca cctgttgtaa aatactgttt ttccaagggt
    19441 tatacactcc ttttcttttt gttgtctata tagttactat attctttatt atttactaaa
    19501 tcaagtagtt attattttag aaaatcaata tggttttgat tttaaagctt aaatgtcagt
    19561 tagatgtcag ttataaatat tttatccatt cccatcataa aaaagatatc ccctcttaac
    19621 ttttggatgg aatattatat ttatacttgt tagagtgaaa attgtaatag gtgctacaat
    19681 aagatattta attaaacata aagttaatta ttgtaataaa aaatctttag ataaattaga
    19741 gttaaagtat ggaaatatag atagagaaaa agctgttaaa ctagaaatat tttatcagta
    19801 tttaatagaa cttgaatata tagaaatgag tttattaata agaagattag atactgcaat
    19861 aacatcatta gcattagtat ctattataac aatagcatca tattatttaa tcagaaaaaa
    19921 tgtgtaatta tgtaagctat ttataataaa acactgataa aattaataat aaaaaggtaa
    19981 t
//
LOCUS       DAKBNN010000056        19950 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000056 DAKBNN010000000
VERSION     DAKBNN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.056"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(49..777)
                     /locus_tag="OUB02_001718"
     CDS             complement(49..777)
                     /locus_tag="OUB02_001718"
                     /inference="COORDINATES: protein motif:HMM:NF013213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCT8885479.1"
                     /translation="MYNLIDTHFHLDFYKNHNEIYNYFNNKKIYVLCVTNQPEVFETC
                     MNIYGSSKYVKFAIGYNPKIINDIPFSKKSFNRNIVKTEYIGEVGLDFKNVNSINRKK
                     QLEIFDYISSIAAKSNKLLNIHCNKAEEEIFTTIKKNGNKKVIIHWYTGDIKYAEKLI
                     DLGCYFSINMNMLKNRKTLEIIKKIPKTRLLIESDGPFSKINGKKYEPEGLIDIYSML
                     ASTIDEADINKLIFNNFKKLLLVK"
     gene            complement(770..2233)
                     /locus_tag="OUB02_001719"
     CDS             complement(770..2233)
                     /locus_tag="OUB02_001719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885480.1"
                     /translation="MKIWINKEEVENLAEEYSEAIVFNMLDRKGNLNTNFEDIWRRFN
                     IQSIDAIYEDLLVIALSVFAVDRKVPRKKISGKYEEEYKYSDNWTRNFEINIPVIELE
                     KWNASKKKLQEFLDFLTGDEWKIDFRFTNKRYKNGKFSTKKYENTNEMIKNRDFDCVS
                     LFSGGLDSFCGALRLLENNKKVFFVTCMEHKLLKKRVNDIYRRVDRGYPKLNKNIYVF
                     SANPKRPMNVSKEFLNKFSEDTSRSRSFLFMAVAIAVASIINPKEGVYIPENGFIGIN
                     LPLTKSRLGSCSTRTTHVWALKRLNEILTLLDIDIPITNFYKYNTKGEIVDEFKNNKI
                     FKECVKLTLSCSHPTHGRMAGATPPINCGYCYPCMIRRASLYKIGFDDTKYIEEYDEN
                     YKLSTSMIKKFGNPYNGRAKDLVALLKFLHVYENNKDKDYYKKELMKMGNLTFEEIKE
                     FDRVCKNSLEELKEMILDVSNKNDSKLIEYIGIKKDV"
     gene            complement(2217..2921)
                     /locus_tag="OUB02_001720"
     CDS             complement(2217..2921)
                     /locus_tag="OUB02_001720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885481.1"
                     /translation="MGTSKGYIPPTNQKWRNAKRAVGLIGKSGYSYSKVQKAVKKYAE
                     AYSETHLGSSSASIVAGNVLGFLSDINKIGFTNTIEKYGLVEFEKLKGLELYNALLNY
                     FASNANTPDNQIVRNSLGDTLDKLHIYELKDLEKINSQEFLMTFLVEFVVKGFEECFA
                     EKIIEHMDDLSKYDDIIRDVEQIVEEKIYVDQVIKNVLELDFTAKSGKEYVSRIINDT
                     FNILKQMEVNSIEDLD"
     gene            complement(2923..4806)
                     /locus_tag="OUB02_001721"
     CDS             complement(2923..4806)
                     /locus_tag="OUB02_001721"
                     /inference="COORDINATES: protein motif:HMM:NF019313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885482.1"
                     /translation="MWVDRESGVDLLSYEPFAELVKNILLDETMNPLTIGLFGSWGAG
                     KSTLLKLVDTKLSNKKEIENKRIATVFLNAWAFEGYDDAKSALMESLLLELQDNVSEF
                     KNVKNKISTLIRRLDFFRIGKFALKYGVPAIGSAFTPVGLAGSAAYLTSQKDNIISDA
                     KDIVREEIKDAQDEAEKKNDVISNIRKFKKEFEELIIESKIDNLVVMIDDLDRCSPER
                     IIETLEAIKLFLSVPKTTFIIAIDDSVIKYSVKKTYPKIDDDDFGIVDNYIEKIIQLP
                     IYIPELSEQDITNYLLFLVIELYFEESVVKKLSSKLTKINAFFSGITLNQQTIKECIP
                     EIKLEEKLLKGKTYQDYIKMFEIVRKVAKVISLELKGNPRQAKRFLNTFLVRKKLGEI
                     YSVGGKSTLDYEVLAQLTALEYLDKNVFRELYKAAMKTKNTDNVQELCIIKNIIENGE
                     EIPKEFFEWDKPSIINWVKNTDINNVSGKELLSYFYLSRESLHINFSAVDELTVEERK
                     AFNEYMKLENTIALKNSLDRMKKNSNLNLDNIIKAIIDSSRNNIKLLKKCVPIYTDYE
                     MYRNKIIEVIVTMKEKDLNMNIIVTLNNLYRIDKNIFKSVIDYFKEIKVDQKKLDLII
                     KED"
     gene            complement(5352..6710)
                     /gene="rlmD"
                     /locus_tag="OUB02_001722"
     CDS             complement(5352..6710)
                     /gene="rlmD"
                     /locus_tag="OUB02_001722"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCT8885483.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSENLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVVLLR
                     RK"
     gene            complement(6885..8645)
                     /gene="pyk"
                     /locus_tag="OUB02_001723"
     CDS             complement(6885..8645)
                     /gene="pyk"
                     /locus_tag="OUB02_001723"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCT8885484.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(8697..9656)
                     /gene="pfkA"
                     /locus_tag="OUB02_001724"
     CDS             complement(8697..9656)
                     /gene="pfkA"
                     /locus_tag="OUB02_001724"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCT8885485.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(9865..13434)
                     /locus_tag="OUB02_001725"
     CDS             complement(9865..13434)
                     /locus_tag="OUB02_001725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCT8885486.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(13591..14538)
                     /gene="whiA"
                     /locus_tag="OUB02_001726"
     CDS             complement(13591..14538)
                     /gene="whiA"
                     /locus_tag="OUB02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCT8885487.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(14555..14980)
                     /locus_tag="OUB02_001727"
     CDS             complement(14555..14980)
                     /locus_tag="OUB02_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT8885488.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(15049..16155)
                     /locus_tag="OUB02_001728"
     CDS             complement(15049..16155)
                     /locus_tag="OUB02_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCT8885489.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(16167..17024)
                     /gene="rapZ"
                     /locus_tag="OUB02_001729"
     CDS             complement(16167..17024)
                     /gene="rapZ"
                     /locus_tag="OUB02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCT8885490.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(17055..17768)
                     /locus_tag="OUB02_001730"
     CDS             complement(17055..17768)
                     /locus_tag="OUB02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCT8885491.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(17772..18686)
                     /gene="murB"
                     /locus_tag="OUB02_001731"
     CDS             complement(17772..18686)
                     /gene="murB"
                     /locus_tag="OUB02_001731"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCT8885492.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(18959..19729)
                     /locus_tag="OUB02_001732"
     CDS             complement(18959..19729)
                     /locus_tag="OUB02_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCT8885493.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKVDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         6086 a         3822 c         1987 g         8055 t
ORIGIN      
        1 caaaacttac tttaaacata ttcacatata atctaataat aaactatatt atttaactaa
       61 aagtagtttt ttaaaattat taaaaattaa cttattaata tcggcttcat ctattgtaga
      121 tgctaacata gaatatatat ctattaatcc ttctggctca tatttttttc cattaatttt
      181 actgaatggt ccatcacttt ctattaataa tcttgtcttt ggaattttct ttattatttc
      241 aagtgttttt ctattcttaa gcatgttcat attaattgaa aaataacaac ctaaatcaat
      301 taatttttca gcatatttaa tatctcctgt ataccaatga ataattactt ttttgttccc
      361 atttttcttt atagttgtaa aaatttcttc ttctgcttta ttacaatgta tattcaataa
      421 tttattactt tttgcagcta tacttgatat ataatcaaat atttccaatt gcttttttct
      481 attaatcgaa tttacatttt taaaatctag tccgacttct ccaatatact ctgtttttac
      541 aatatttcga ttaaatgatt tttttgaaaa tggtatatca ttaattattt taggattata
      601 tccaatagca aatttcacat atttacttga accatatata ttcatacacg tttcaaaaac
      661 ttccggttga ttagtcacac ataaaacata tattttttta ttattaaaat aattatatat
      721 ctcattatga tttttataaa aatctaaatg gaaatgagta tctattaaat tatacatctt
      781 tcttaattcc tatatattct atcaatttgg aatcattctt atttgataca tctaaaatca
      841 tttcttttaa ttcttcaagt gagttcttac atactctatc aaattcttta atctcctcaa
      901 aagtaagatt acccattttc attaattctt ttttataata atccttgtct ttattatttt
      961 cataaacatg taagaattta agtaaagcaa ccaagtcttt agctctacca ttatatggat
     1021 tcccaaattt ctttatcata gaagtagata gtttataatt ttcatcatat tcttcaatat
     1081 atttagtatc atcaaaccca attttatata gagatgctct tctaatcata catggatagc
     1141 aataaccaca attaatcgga ggtgtggcac cagccattct tccatgtgtt ggatgcgaac
     1201 atgatagagt taattttaca cattctttaa aaattttatt atttttaaac tcatctacaa
     1261 tttcaccttt tgtattatat ttataaaaat tagtaatagg gatatcaata tctaacaatg
     1321 tcaatatttc atttaatctt ttcaatgccc atacatgtgt tgtacgagta ctacatgagc
     1381 ctaaacgact ttttgttaac ggcaaattaa tccctataaa tccattttcg ggaatgtaaa
     1441 ctccttcttt aggattaata attgatgcaa ctgcaattgc tactgccata aataaaaaag
     1501 atctacttct acttgtatct tcagaaaatt tattcaaaaa ttcttttgat acattcattg
     1561 gtctctttgg atttgcagaa aatacataaa tgtttttatt taatttagga tatcctctat
     1621 ccacacgtct gtaaatatca ttaaccctct tttttaacaa tttatgctcc atgcatgtta
     1681 caaaaaagac ctttttatta ttttcaagca atcttaaagc cccgcaaaat gaatctaatc
     1741 caccagagaa aagactaaca caatcaaaat ctctattttt aatcatttca ttggtatttt
     1801 catatttttt tgtgctaaac tttccatttt tatatctttt attagtaaat ctaaaatcta
     1861 ttttccattc atctccagta agaaaatcaa gaaattcttg tagctttttt ttacttgcat
     1921 tccatttttc taattcaatt acaggaatat taatttcaaa atttcttgtc caattatcac
     1981 tatatttata ttcttcttca tactttcctg atattttttt tctaggaact tttctatcta
     2041 ctgcaaatac tgataatgca ataactaaca aatcttcata aatagcatct atgctttgta
     2101 tattaaatct tcgccagata tcctcaaaat tcgtattcaa gttccctttt ctatctagca
     2161 tattaaaaac aatagcttct gaatattcct cagctaaatt ttcaacttct tccttattaa
     2221 tccaaatctt caatgctatt cacctccatt tgttttagta tattaaatgt atcattaatt
     2281 atccttgata catattcttt tccagattta gctgtaaagt ctaattcaag aacattttta
     2341 ataacttgat ccacatatat tttttcttcc actatctgtt ctacatccct aattatatca
     2401 tcatatttac tcaaatcatc catatgctca attatctttt ctgcaaaaca ctcctcaaat
     2461 cctttaacaa caaattctac taaaaaagtc attaaaaatt cttgcgaatt aattttctct
     2521 aaatccttca actcatatat atgtaattta tctagcgtat ctcccaaact atttctaact
     2581 atttgattat ctggtgtatt agcattactt gcaaagtagt ttaaaagtgc gttatacaac
     2641 tcaagtcctt ttaatttttc aaattcaact agtccatatt tttctattgt attagtgaat
     2701 ccaattttgt ttatatcaga taaaaatcct aatacattac cagctacaat actagctgag
     2761 cttgaaccta aatgtgtttc gctatatgct tccgcatatt tttttaccgc tttctgtact
     2821 tttgaatatg aatatccact tttcccaatt aaaccaactg ctctttttgc atttctccac
     2881 ttttgattag ttggcggaat atatcctttt gatgtcccca tattaatcct ccttaataat
     2941 taaatcaagc tttttctgat ccacctttat ttctttaaag taatctatga cagatttaaa
     3001 aatgttttta tcaattctat atagattatt taaagtcact attatattca tatttaagtc
     3061 tttttccttc attgtaacaa ttacttctat aatcttattc ctatacattt cataatccgt
     3121 ataaataggc acacatttct ttaacaattt gatattattt ctactactat caataattgc
     3181 ctttatgata ttatctaaat ttagattact attcttcttc attctatcta aagagttttt
     3241 taatgcaatt gtattctcta atttcatata ttcattaaat gcctttcttt cttcaactgt
     3301 tagttcatct actgcactaa agtttatatg taaagattct ctagataaat aaaaataact
     3361 taacaattct tttcctgaaa cattattaat atctgtattc ttaacccaat taattataga
     3421 tggtttatcc cattcaaaga actcttttgg aatttcttct ccattctcta ttatattctt
     3481 aataatacac aattcttgaa cattatcagt atttttagtt ttcatagcag ctttatataa
     3541 ctctcgaaaa acatttttat caaggtattc taaagctgtt aattgtgcta atacttcata
     3601 atcaagagtg ctttttcccc caacactata tatttctcca agcttttttc taacaagaaa
     3661 tgtatttaaa aatcttttag cttgtcttgg attacctttt aattctaaag atattacttt
     3721 agcaactttt cttacaatct caaacatttt tatataatct tgataagtct ttccctttaa
     3781 taatttttct tctaatttaa tctccggaat acattctttt attgtttgct ggtttaatgt
     3841 tattccgcta aaaaacgcat ttatctttgt caatttactt gacaattttt ttactaccga
     3901 ttcctcaaaa tataattcaa taactaaaaa taataaataa tttgtaatat cttgctctga
     3961 taattctgga atataaattg gtaattgaat aattttttca atataattat ccactattcc
     4021 aaaatcatca tcatctatct taggatatgt ttttttcaca gagtatttta ttacagaatc
     4081 atctatagct ataataaatg ttgtttttgg tactgataaa aataatttaa tagcttcaag
     4141 agtttcaatt attctttccg gactacatct atccaaatca tcaatcataa ctactaaatt
     4201 gtcaattttt gattctatta ttaattcttc aaattctttt ttaaattttc taatattact
     4261 aataacatca tttttctttt cagcttcatc ttgtgcatct tttatctctt ctctaactat
     4321 atcttttgca tctgaaatta tattatcttt ttgcgatgtt aaatatgcag cacttccagc
     4381 taatccgact ggagtaaaag cacttcctat tgctggaacg ccatatttta atgcaaactt
     4441 tccaattctg aaaaaatcta gtcttctaat caatgttgat attttatttt tgacattttt
     4501 aaattcagat acattatctt gtaattctaa taataacgat tccattaaag cgcttttggc
     4561 atcatcatat ccttcaaaag cccatgcatt caaaaatact gtggcaattc tcttattttc
     4621 aatctctttt ttattactca attttgtatc aactaatttt aaaagcgtag acttaccagc
     4681 tccccaactt ccaaataaac caatagtcaa tggattcatt gtttcatcaa gtaatatatt
     4741 ttttactaat tctgcaaatg gctcatatga caataaatct acccctgatt ctctatcaac
     4801 ccacataagt ttacctctct ttcatattaa taatttttat aataaaatta taaaaactat
     4861 tcttcttttt ttaattgcat aattgtttta attactttta acaaattaaa tcttcccatt
     4921 ttttctataa ttactattta ttatatatct ttctacttaa tatttccata atacttcaag
     4981 tgaaaagtgt ctaactttgc ttaaattata aaaaatgtct ataatttatt ttaattttct
     5041 ttccaatttt atataaagaa attgaattaa actactcttt tattatttta taaataattc
     5101 attaaccaag cttattaaat tttattttct aatatattat tatataaaaa tagacattga
     5161 atatataaat tcaatgtcca ttctatgatt aattacttta tttcaaattt cttacttagt
     5221 atttccacac tccatatata taactacaga cacgtcaaaa tatcttttca attctttatt
     5281 cctgatttta ttcccctctg tcctaaatat tatatgtcta tgaaagtggc taaaactcag
     5341 tatttctagc attattttcg acgtagcaac actacagtct ccacatgtgt agaatgaggg
     5401 aacatatcta caggttgtgc ctcaacacat ttaaatccgt gaacatttag atagtctaaa
     5461 tcccttgcta aagtagaagg attacaagaa acatagacaa ttctttccgg attcattgaa
     5521 acaatagtct ctaatacctt ttcgtcacat ccctttctag gtgggtctac aactactaca
     5581 ttggcagtct taccctctct atacatctta ggtacaattt cctctgcctt tccaacataa
     5641 aattcagcat tatgtatatt gtttaattta gcatttattt tagcatcttt tatggcatca
     5701 tctataattt ctattccata aaccttttta gccttttgtg ccaaaaataa agatattgta
     5761 cctatcccac agtaaatatc aaaaacagta tcagtatcat ttaaatttgc atattcaaga
     5821 gccttatcat acaatacttt agtttggaca gggtttactt ggaaaaatga taatggtgaa
     5881 atctcaaaca ctaagtcacc tatataatcg tttatttttc catctccata tataactata
     5941 ttttctcttc ctagaatagc attcgtatct ttttgattta catttacaac caaagtctta
     6001 aatccaggta tattttcctt aagcactgaa gctaattctt taagatatgg aagtttgttt
     6061 ccatttgcta ctaatacaac cattacttct ttagttgtaa aacctatttt tgttactaaa
     6121 tgcctaatca accccgtgtg agtcttttca tcatatatac ttaccttata ggcccttata
     6181 tatgttttta ttattttaac aattttatca tttatctcat gttgtattac gcatttttct
     6241 gtcgatatta catcatgagt cttcttttta tagaagccaa taattggaat accattattc
     6301 ttttgtattg gaaattgtgc tttatttcta tatctatatg gattatccat tcctatagtg
     6361 tcatgaactt ctacatcctc taacttacct attctcgaaa gtacttgttt aacttcattt
     6421 gttttcattt caagttgttt attataatct aagtcttgta tctgacaccc tccacaatcc
     6481 ctaagattct cactacacac tcttttcacc ctgaatggag acttttctat tatctcaaca
     6541 atatctccta cagcataatt tttctttact ttattaatcc taactttaac tttatcctgt
     6601 attaatccgc cctctacaaa aactgtaata ccattgtact ttcctactcc tactccgcct
     6661 tgaccaatgt ctactatatc tacaatatac atctcgtttt tcgaaagcat aaatcaccct
     6721 ccaatcaatc tattatattt aaatctttac aaactttttt ctttaaattt atattaaaat
     6781 attgctatta attaaactat attttttact aagctacaat attttatttt accaaaaaaa
     6841 aaggagctta taaagcccct tagtaaataa atttattatt tttcttataa aactctagtt
     6901 gaacctctat ataccacacc tcttgatgca tccacagtta caacttctcc atcttttact
     6961 agatttgtta tatcagttgc agacacaata actggtatat ctaaatttat acctacaata
     7021 gctgtatgtg atgtcattcc accattctca gttactacag ctcctgcttt ttctatataa
     7081 ttgttcattt cagcatcagt agcagtagaa acaagtatgt cgccttcatt aaagtctaca
     7141 caagcatttc cattctttat tatacgaact ttaccttcta ccgtttgagt acctacacct
     7201 ataccttgca cgatttcttc gcttattaca tgaaccttta ttaaattagt agttccactt
     7261 actcctactg gtactccagc agttataact actaattccc cattatctat ataatcagct
     7321 tgtctagctg cctcaattgc cttttctata acttcatctg tatttcctgc aacagaagac
     7381 ttaactggat atactcccca tgtaagagct aatttattca tagtcttttc attatttgta
     7441 gtagctatta ttggagattt tggtctaaat ttagatacca ttctagcagt atatcctgaa
     7501 gaagtagatg ttattatagc tgatgcattt aaatctactg ctgttgtaca agttgcatgg
     7561 cttattgcat ctgttacagt aacattgtta gtaccatttt cctttaataa tctatcataa
     7621 tctaaagtct cttctgttct ttttgctatt gtagccatca tttttactgc ctctacagga
     7681 tattttccag cagcagtttc tccagataac attatcgcat ctgttccatc atatatagca
     7741 ttggcaacgt ctgtaacttc tgctcttgtt ggtcttggat ttcttatcat agaatcaagc
     7801 atttgagtgg cagtaacaac tggctttgct aattcattac atttttttat catcattttt
     7861 tgaactattg gcatctcttc agttggtatc tcaacaccta agtcacctct agccaccatt
     7921 ataccatcag aaacttttaa tatttcatct aggttctcta caccttcttg gttttctatt
     7981 ttagatatta tttgtatatc agttgcatta ttgttctcta atacttctct tattgctagt
     8041 acatcagatg cctttcttac aaaagaagca gctatataat ctattccttg acttattcca
     8101 aactctatat cacttatatc tttaggagtt attgctggta aatttatctt aacaccaggt
     8161 acatttactc ctttatgatt tttaactatt cctgaatttt caacaacaca aactatatct
     8221 tctccattta tttcttttac tcttaagcca actaaaccat catctattag tatagaatct
     8281 cctgatttaa cgtcctcagc aagaccttta tagcttactg tacacatttc tttagtcccc
     8341 ataatatctt tcatagttat agtgaacttt tgcccttctt ctaaaaatac ctctgggtct
     8401 tcaaagttac ctgttctaat ttctggacct ttagtatcta ataatatagc aactggctta
     8461 tttaattttt ctctaacttt ctttgccata tcaattcttt ctttatgttc ttcatgcgaa
     8521 ccgtgagaaa agttaaatct acacacattt agaccatttt gcataagttg agttaaaact
     8581 tcctcacttt gtgatgctgg acctagtgta caaacaattt ttgtcttctt tacattattt
     8641 aacatatttg ctcctcctga gatattattc cacagattta tttttaatac taaaacttat
     8701 atagataata tttttgccat ctcataagct tctttatcaa atactttctt ttgagctaaa
     8761 gcttcactta tttctaaatc aattattttg ttttctttta ttcctacaac tctagctgat
     8821 tttccatcta ataataattc tactgctctt actccaagtc tacttgctag tattctatca
     8881 gatacagttg ggcttcctcc tctttggaca tgccccaata cagtgactct taaatctgct
     8941 cctgactctt ttttaagttc cttttctaaa tcactagcac ttccgactcc ttctgctaaa
     9001 acgattatac tatgtctctt acctcttttt tgagtagtct ttaatcttag agcaacttca
     9061 tctactgtaa tttctacttc tggtactatt atagtttccg ctcctcctgc aagacctgca
     9121 tataaggcta aatctccaca gtgtcttccc ataacttcta ctatgttaac tctttcatga
     9181 gatgatgatg tatctcttat cttacctata gcatctataa tagtattcat tgcagtatca
     9241 aatcctatag tataatctgt atatgctaaa tcgttatcta tagttcctgg tatgcctata
     9301 gctgggaatc ctaagtcact aagtttttgg gctcctgcaa atgaaccatc tccaccaatt
     9361 acaactaagc aatcaatttt atacttttta agaattttta ctgcttttaa tctaccttct
     9421 tctgttttaa attcttcaca tctggaagat tttaatatag ttcctcccct gtgtataata
     9481 tctccaacag aagataagtt catttcagtt aaatcttctt ctagtaatcc cttatatcct
     9541 ctattgattc catatacttt gcatccataa tatattgctg atcttacaac cgctctaatc
     9601 gcagcattca ttcctggagc atctcctcca cttgttagta atcctatagt tttcattttt
     9661 tacctccttg gattttatgt caatttttta ttataatcat tgttattgta ttctaatgga
     9721 ctttttttgt ccctatatca ctttttaatc ccatcaagac taaaaaaatt aaatgtcctt
     9781 aaatgtccat tttgtgatat tatatcacaa aacggacaat atttttcata ttattataat
     9841 atatttagat ttttatataa aaaatcaagc ttttactttt atgttatctt taggaagtaa
     9901 ctcttctaag ttatttataa attcatcaca tatacaagtc aatgccccgg tcattttata
     9961 tgctttacgt ttaccattta tattttcatc catagggtac aagtatatac tgtcattacc
    10021 tggatattgt ttggctattt ctacaatatt attagttgta tcagtatcag acatactatc
    10081 tattttcaaa tacaattttt taccactttc tggcttaata ttttctacat ttctagttct
    10141 tcctgaattg tagtttctat ctgcaaagtc agtatcatca tttatatctt ttatctctct
    10201 agctataagt tttgcatttt cgccttcttt tatacttaat gttcctttta catatattat
    10261 attatcttca ttcaaaatga cattatactt ttgtaataat tgaggaaaaa ctattatttc
    10321 tatagcacca tacaaatctt ctaattcaat aaatgccata atttcatttc gttttgttgt
    10381 tttaatagtt ttagttacaa tcattccacc cattataaca tctgtttcat tcatttgtag
    10441 gtatgactct tcttcctctt ttaatgaatt taattttcca ttatccatag atgttttttc
    10501 ttgtagttct ttttcaaatt gtgataaagg atgtccactt acatacattc ccaagacttc
    10561 tttttcaagg ttaagtctct ctctttcttc gtactcttgc cttttaggca aactgtatat
    10621 ttgttgaaaa tcactatctt cctcactatc tccaaaagca tcaaataaag aaatttgacc
    10681 ttgaacattt ttctttctat ccatagatac actttctaat aaactttcat aaccagccat
    10741 cagactagct ctattttcac ttatattatc aaaagctcca cattttatta gactttctat
    10801 tactctctta tttgtatctt tttggtctaa tcttttgact aaatctacta aatctacaaa
    10861 taatccatta gattctcttt cagcgattat attgtttatt atatttacac caacattttt
    10921 tacagctgct agaccaaatc ttatattgtc gccttcaact gaaaactttg aaaaactttt
    10981 gtttatatct ggaggtaata catctatttc tagtgcttta cattctctta tatactcaac
    11041 taccttatca gtatttccca ttacactggt tatcaatgct gccataaatt caactggata
    11101 atatgttttt agataagctg tctcatatgc taaaacccca tatgcagctg catgtgattt
    11161 attaaatgca tatctagcga agtctatcat gtcatcaaat attttattag ctatttcctc
    11221 ttctacacca tttctgatac accctgcaat ttctatatta ccatcttcat caaatttacc
    11281 atgaataaaa tattgtcttt cttcttccat cacgtccatc tttttcttac tcatggctct
    11341 tctaactagg tcacttcgtc cataactata accacctaaa tctctaacga cctgcataac
    11401 ctgctcttga tataccaaac aaccatatgt aacttccatt ataggcttta atttttcatg
    11461 tagatatgtt acatcttttg ggttgctttt attttttata tatgcaggta tagaatccat
    11521 tggacctggt ctaaataggg atattcccgc tacaatatct tcaaaattgt ctggttttag
    11581 ctgtttcata aaacttctca tgccagcact ctccaattga aacaccccta gtgtatttcc
    11641 tgatgaaagc atttcatata ctttcgggtc gtcataatcc atctttgaaa agtctatttt
    11701 ttctgtgtag ttttttataa ctctattttt ttctattaaa tcaagagtat ctcttataac
    11761 tgtaagagtt cttagcccaa gaaaatccat ctttaaaagc ccaagttctt caagtgttgt
    11821 catagtaaac tgtgttgtga ttgcatcttg atgtttgtac aaaggtacat attcatctac
    11881 gggatttttt gatataacaa ctcctgctgc atgagtagaa gcatgtctta gcattccttc
    11941 tatctgtttt gatatattta ttatttcttt tgtttccggg tcttggtcat acaattcttt
    12001 aagattagga tttgtatcta gggccttatc tattgtcatt cctaaagcaa atggtatttc
    12061 cttggctacc ttgtcaactt tattgtagcc tatatccaat acccttccaa catctcttat
    12121 tgcagctttt gcacccattg taccaaaagt tattatctgg gctacatggt catcaccata
    12181 ttttctcttt acataatcta taacttcttc tcttctctca taacaaaaat ctatatctat
    12241 atctggcatg gatatacgtt ctggatttaa aaaacgctca aataatagag agtactttat
    12301 tgggtctatg tcagttattt taagtgtata tgctactata gaaccagccg cactgcctct
    12361 acctggtcct actataatat tattttcttt agaaaaattt ataaaatccc aaacaattaa
    12421 aaaatactct acataaccca ttttttcaat tacatctaat tcataattta atctctccag
    12481 tatctcattg ctaggattat catatctctc ttttaatcca ttaaaacaca actcccttaa
    12541 ataagtttct ggtgtatatc cttctggaac atcatactta ggaagatgta tagtattaaa
    12601 atcaaactcc acattacaca tatcagctat tttcacagta ttgtcaatag cctctaatgc
    12661 atatggaaat aattcttcca tttcttctct tgattttaaa taaaactcat cactaccaaa
    12721 tctcattcta tttgggtcat ttaatgtttt tcccatttga atacacataa gaacatcatg
    12781 tattttcgaa tcttctttat ttacatagtg aacatcatta gtagcaacta gtggtatacc
    12841 agtctctttt gataatttta ctaaaccaga gtttacttct ttttgctcag gtaaattgtg
    12901 atcttgaatc tccaaaaaga aattttcttc tccaaatatg tctctgtagt caagagcaaa
    12961 ttgttttgct ttttcataat ttctattcat taaagcacgt gctacatctc ctgcaaggca
    13021 tgcagaaagt gctataatcc ctttgttatg ctttttaagt tcttccatgt caactctagg
    13081 cttatagtaa aaaccttcta cgtatgaagt tgaaactatt tttattaaat ttttatatcc
    13141 ttccatattc ttagctaaca gaactaaatg tccttgatat ttgtcatagt ttgggtcttt
    13201 atctctcaat ccacgtgcag ctgtatagac ttcgcaccct ataattggct ttattccttc
    13261 ttttatagct gttttgtaga aatctataac accaaacata caaccatggt cagttatagc
    13321 tattgagctc atgtttagtt ccttagccct ttttataagt tttttcaccc tcgaaaaccc
    13381 atcaagtaaa ctatactcag tatgtacatg aagatgagca aaatctttgt tcattaaatc
    13441 acctcctgct ttataatttt aattttacca caaatatatg tcctaaaaca aaaacagaga
    13501 cttttatctc aaaataggaa ttaacttcaa cgtataccat tatatgttat aaattaatgc
    13561 ttattctgag attgtcccct caatttattg ctatgatttt tttttcaaat ctgttgcaat
    13621 ttcttctatt tttctaagtc tatgattgac tccagatttt cctattggtg gttctaacat
    13681 ctctccaagt tctttgagag tcatatcctc atactcgatt ctaatcttag ctatctcttg
    13741 taaattttct ggcaaactgc taattcctat agtctcttgt atgagcttaa tattttctac
    13801 ttgtcttact gctgcattta ctgttttaga aaggttagct gtctcacaat ttactatcct
    13861 gtttacatta tttctcattt cttttactat tttagtattt tgaagactta aaagagcgtg
    13921 gtgtcctcct ataatactca acaaatctga tatttgttca ctctctttga tataaacaac
    13981 gtgattattt tttctagcca caattttact attaaaacct aaagagttta taagttcttt
    14041 tagagaatct gcaaactcct cattattagt tacaaattcc atatgatagt tcttttctgg
    14101 gtcgcttata gaacctccac caagaaaagc tcctcttatg aaagctctct tacatccttc
    14161 atgcttaatt aagttctctg aaattttgtt tatagtaaaa aatccttctt tatcttcaag
    14221 tatacctaac ttttttaata acatctctga acccatttcg cttgttacca ttaatacata
    14281 actgttattt ctctttagca tttgattctt atttactgag attgtagtat taatattaaa
    14341 attctttttt aataatttaa atactttacg ggctattgaa tttaactctg ttgttatctt
    14401 tagatttagc ttcttatatc caacaatttg tatacttccc cctgatttaa ctagagctga
    14461 caattcagct atattacaac attcattccc agatataact cttgctagtt catttttggt
    14521 ttcagttgaa aaagacatat ctatcaccta ttaactaatt tttctttaac ctttttttta
    14581 tttcttgtaa ggcaaccttt attatctcct tttcatcatc atacctagct aagtctacta
    14641 ctctatctag gtggataaat ttagcatcaa taaagccttc ctctttttgt ggaatagtat
    14701 ccatattctt tgcttgcatc aaataccaaa aaactgtttt atgaactgct ctattttcat
    14761 cccagttttc tttgaatgta tagtgtattt cacctaaata tttcaatata tctgctttta
    14821 ctccagtttc ttcacttact tcacgtaatg ctgcctcttg gttattttcc ccttcttcta
    14881 cttttccctt gggtaacacc caatctccat tgaacttcct tagtaaaaga atcgtattgc
    14941 caaacaggac tacaccacca gcactaacct cttctctcat cgatgttcac tccttaaaac
    15001 taacataatg ataaggattt ctacacaatt ttttaaaatc cttttttact aacttttgtt
    15061 gtaattatga tttagggcta actcaataac tatgttagat atatactttg catcatgtct
    15121 tatataatta ttttttattt caattaaatt cttctctaca gtttttatac ccatctcttt
    15181 taatttatct ttttgctttt tatctaacaa gacttgctct gctccatctt gtttatataa
    15241 gtcaagcatt tcttctggca atatctcatt atttgcgatt acataatcta ttaaattctc
    15301 atcagtgtgt tttactatag cattaacatg ctctaataca ttgtaccctt ctgtctcacc
    15361 tggttgagtc attatatttg ctatatatac ttttggagct acagacgatt taatagcgtc
    15421 tacaatcccc tctacaagta aattgggtat tatacttgta tataaacttc caggacccat
    15481 aattataatg tcagcatcat aaatagatgc aataacatcc tttaatggtt ttacatcttt
    15541 tggatttaaa aaaattttat caatagagct tttttgaatt tttgactctt caggtatact
    15601 agattctcca tttattatat tgccattttc taatttggca actaaattta catcttccaa
    15661 agttactggt aaaactcttc ctgttatagc aaaaatttca cttaatttat atacagcttt
    15721 ttcaaagttt ccatacaacc cattcatagc agccaaaaac aaatttccaa aactctgacc
    15781 ttttaaaagc ccttctgtaa acctatattg cataacttca ttcattgttg gctctatatt
    15841 tgcaagtgct aataaacagt ttcttatgtc tcccggtggg agcattccta agtcttctct
    15901 caatacacct gaacctccac catcatcagc aacagttaca attgcagtta tattttgcgt
    15961 agtgtgttta aggcctctta aaaataccga ttgaccagta cctccaccta tcacaaccac
    16021 atttggttct ctattttcaa caatagacct ttgaaatagt tttacttttt cattttttgc
    16081 ttttttatat ataaataacg atacaattaa tgcaatccaa cctaaaattc ctacaataat
    16141 aagtaattta gccatgtttg cctccattat cgcaacatag agtctctatg tttctttaca
    16201 actctatatc ccttattaga taaatcctcc gctataagat ttgttatagt aactgaccta
    16261 tgtcttccac ctgtgcatcc aacaccaatt actagatgtt gttttccttc ctctatatat
    16321 tgaggtacta aaaaatgaat catatctaaa agtttcacat aaaattcttc actgatttta
    16381 gaattcataa cataatctct aacttcttta tcatcaccag ttttagctct taactcttca
    16441 acataatagg gatttggaag aaatcttaca tcaaatacta aatctgcatc agcaagtata
    16501 ccatgtttaa atccaaaaga tactacagaa atagttaggt ttggattatc ttcaccagaa
    16561 gaatatattt tctttatttc ttccttaagg tctttaggtt tcatgttagt tgtatcaatt
    16621 atacatgtag ataactcctt taaaggttcc attatctttc tttccatttt tataccttct
    16681 ggaatctgca tatcttttgc caatggatga tttcttcttg tcattttata tctctttaac
    16741 aatacatcat cattacaatc caaatatacc atttcatatt cataattttc tttttctaaa
    16801 taatttaagc tttcatgaag cgcttcaaaa aactttctcc ctcttatatc tatccctaga
    16861 gcaactttat caatacttga atttggttga taacataatt ctgcaaattt agttatcaaa
    16921 gcaggaggta gattatctac acaataaaat cccatatcct ctaaagctct cattgtctca
    16981 ctttttccag atcctgaaag acctgtaact ataacaaatt tcattgctaa ctcctccctt
    17041 ttatcttatg tttattatat tataatgttt tctactaacg atatatccag attagagtca
    17101 gatatagtct tcatagctag ttcaaaatca ccaactcttt ctggacaatg tgcatctgag
    17161 cctattataa atttattttc tagccctttt attttattta attgatttac attcaaaaaa
    17221 gaatggctac tatttatctc aagtctagta tttgtctttt ttgctgtctt tgctacttct
    17281 tctatataca catcgccttt atctcctgga tgagtaatta taaaaatatc attattactt
    17341 atcgcattta ttacagcttt tgtattatat tcttttgctt tttcagtttt aaaaatatag
    17401 ttattacaat gatttaataa gcttcttaat gatgttggag tagaaccaaa gtgatatcca
    17461 gccataatat aatcaaaatc tctaataaat ttatcatcca tatctatatt tcctttatca
    17521 tctaatatgt tacactccat acctaaaagt atatttatat cagtgtactt atcatttaaa
    17581 taatctactt cttctctcat tttgagaata tcttttttct ttacaccata tgcaatatgt
    17641 ttataactat ggtcagatat tcctattgtt tttattcctt tagaaatggc tacctttaca
    17701 ttatcctcaa tagtcccttt gccatgacta tataatgtat gtgtatgata atcagccagt
    17761 attttcattt gttaatcctc tccaagtatt ttgacttctt cttccaacat aaccccaaac
    17821 ttggcatata cagcactttt tataacatat attaaatcca aaatatcttt agcacttgct
    17881 tctccttgat tgactacaaa tccacaatgc ttctcagata cttgtgctcc tcttaaagta
    17941 agacctctca atccagtttc ttctattaat ttacctgcaa aatatcctgt tggtctctta
    18001 aatgtacttc ctgcactagc aaaatttaat ggctgtttag ttattcttct cgtcgctaaa
    18061 tcatccatta aactttttat ttcatcataa tttccttctt gtaactctaa ttctgctgat
    18121 aaagctatat atccgttttt cgatattata ctttttctat acccaaattc catctgttca
    18181 tttgataatt caaatatatt gccttccata tccatcagtc ttactgactt cactatgtcc
    18241 ttcatttcac caccatatgc tccagcattc atggctaatg ctcctcccaa tgtgccaggt
    18301 attcctgatg caaactcaaa cccttttaat tcttgctttt ggagagcttt ccctaaaact
    18361 gataataaag ctcctgactg agcagtcatc ctagtatcat ttatctcata agaattgaaa
    18421 ttatcagcta attctattac cactcctctt attccaccat ctttaattaa aatgtttgaa
    18481 ccatttccaa ttattaaata cggaatagat tcttttttta ccaacttaag tacatttttt
    18541 aattgttctt cagttctagg tctaactaat atatctgctg gtcctcctac cctaaatgaa
    18601 atatgttttt tcataggttc atcgactttt atatcctctt tacttaatat atttaataaa
    18661 tttatatata tatcttgatt attcattgta tttcctccca aattatactg aactttagct
    18721 gtcattatta tttaaattac attagtatat agtattaact atattcaata atatatgatt
    18781 ttgcttatca tatagatttt atattttaag cttcttaatt acaaactata ttcaataaat
    18841 atataataaa gtaaaaaaaa aaaaataaca acatataaaa gaatataaat aatttaaaaa
    18901 aagcatcaat atcactgata tcaatgcttt aaaaggcaat ttaattcgta ttatttatct
    18961 aactattttt gacatagcaa agataataaa tccctccaac tactatggaa cctccaacga
    19021 tatttccaag cgttacaaat accaaattag ttataaattg aaccatagtt atatttgctc
    19081 ctaatgccat tcccattggt ataaaaaaca tgtttgcaac actatgttgg aatccacata
    19141 atacaaatag cattattact gtccaacatg aagccaattt tgacattata tcttgagatg
    19201 ctgtagccat ccatacagct aaaacgacta gaatattaca aagtattgct tttaaaaaca
    19261 ttgatgctat agttgaatta accttagctt ctgccacagc tatagctttt gtagctgcat
    19321 ctcctgtcaa tgtagaagaa taaaacacaa gtgcaactag caaaattgaa cctactaaat
    19381 ttgcaaaata aaccagtccc cagtttctta acatttttcc taatgttatt tttttgtcca
    19441 taagtgcaat agccattaat gtgtttccag tgaataattc tgctcctgcc actactacaa
    19501 gcataagacc tactggaaag accatagcac ttaaaaagct agctacccct gggtctacct
    19561 tgccaaaagt ttgactaact atcatactcc ccaatgcccc aaaacctata aatgctcctc
    19621 caaataaacc taatagaatt gcattttgtg tcgaaatatt tgctttttta atgcctgcac
    19681 ttatagttgc ttgtgcagtt tccccaggtg tcaagaatcc tttactcata aatccttccc
    19741 ccttaaaaat tatactttcc cttgtctttt tatggcaata attatataat aacaatatga
    19801 tatataataa attttattaa cataaaaaat atactatata cttatatata cttgtctcat
    19861 ataattatac tatttattat ataacaactt tgttaatttc gaaataattt atattgaatt
    19921 tgaaaaattt accttttgat tatttatttt
//
LOCUS       DAKBNN010000057        19896 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000057 DAKBNN010000000
VERSION     DAKBNN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.057"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            173..514
                     /locus_tag="OUB02_001733"
     CDS             173..514
                     /locus_tag="OUB02_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HCT8885494.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            665..1078
                     /locus_tag="OUB02_001734"
     CDS             665..1078
                     /locus_tag="OUB02_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HCT8885495.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1280..1921
                     /locus_tag="OUB02_001735"
     CDS             1280..1921
                     /locus_tag="OUB02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCT8885496.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1945..3198
                     /locus_tag="OUB02_001736"
     CDS             1945..3198
                     /locus_tag="OUB02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT8885497.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3244..3894
                     /locus_tag="OUB02_001737"
     CDS             3244..3894
                     /locus_tag="OUB02_001737"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCT8885498.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNAKEVIQIAENSDIDFKDNNIYLDNEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3945..4541
                     /locus_tag="OUB02_001738"
     CDS             3945..4541
                     /locus_tag="OUB02_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCT8885499.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4554..5393
                     /locus_tag="OUB02_001739"
     CDS             4554..5393
                     /locus_tag="OUB02_001739"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCT8885500.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5638..6792
                     /locus_tag="OUB02_001740"
     CDS             5638..6792
                     /locus_tag="OUB02_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HCT8885501.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7098..8795
                     /gene="ade"
                     /locus_tag="OUB02_001741"
     CDS             7098..8795
                     /gene="ade"
                     /locus_tag="OUB02_001741"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCT8885502.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            9147..9611
                     /gene="bcp"
                     /locus_tag="OUB02_001742"
     CDS             9147..9611
                     /gene="bcp"
                     /locus_tag="OUB02_001742"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCT8885503.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            9621..10364
                     /gene="yaaA"
                     /locus_tag="OUB02_001743"
     CDS             9621..10364
                     /gene="yaaA"
                     /locus_tag="OUB02_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCT8885504.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            10492..13149
                     /locus_tag="OUB02_001744"
     CDS             10492..13149
                     /locus_tag="OUB02_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCT8885505.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      13203..13305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13415..14680
                     /locus_tag="OUB02_001745"
     CDS             13415..14680
                     /locus_tag="OUB02_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HCT8885506.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14710..15600
                     /gene="metA"
                     /locus_tag="OUB02_001746"
     CDS             14710..15600
                     /gene="metA"
                     /locus_tag="OUB02_001746"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCT8885507.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            16091..16534
                     /locus_tag="OUB02_001747"
     CDS             16091..16534
                     /locus_tag="OUB02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8885508.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            16565..18382
                     /gene="ftsH"
                     /locus_tag="OUB02_001748"
     CDS             16565..18382
                     /gene="ftsH"
                     /locus_tag="OUB02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCT8885509.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            18560..>19896
                     /locus_tag="OUB02_001749"
     CDS             18560..>19896
                     /locus_tag="OUB02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCT8885510.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVS"
BASE COUNT         7964 a         2152 c         3556 g         6224 t
ORIGIN      
        1 aaaaaaagtc tttttaactt taaaaattaa tgttaaaaaa taaacttata cattaatatt
       61 aataataatt atttaaatat cttaagtaaa tcagtcttac atatatttcc gatagattat
      121 attgataaaa aacactaaaa ttaaattaat aaaagtactt ggagggatgg aaatgaaaga
      181 ggaaaatttt attaatgttg aatacgagga taaacgtttt tcaattaata gtaaagaagt
      241 tttgtacata gaaaagcaag agtatgaagt aaagatttat actgaaagtg gtaattatga
      301 agttggagaa aatgctcctt gtataagaaa actattgaaa gaagaaaatt ttttcaaatg
      361 cagtaatagt tatattgtaa atctagacaa gtgtgaattt tatctcagca ataattgtat
      421 ttttgtgaat aataatttta taagaatttt aggttcagaa agaaaaaaga aattaaaaaa
      481 gaaattagaa aaattatatg attatgaaga ttaatattgg gaggctgtat tttatacagc
      541 ctctttattt attgattgta ttattaataa attttaaatt atgataaaat ataaaacaag
      601 taacttattt cagaataaaa tactttttaa aagaaaggga cgagatatgg gagattataa
      661 taaaatgatt gaattaaaga ataacttagg tatcagtgaa agaaagttaa aatatccttg
      721 tatctataaa cattttaaag gaaaatatta tgctacaatg ggtcttagca aggctattga
      781 tgatatagaa aacatatgtg aaatatatgg taaagaaaat ttaatccaaa acagaaataa
      841 atataaatta gttattagac atactgaaag agaagaagat atctatgttt atagggattt
      901 agatggaaac ttttatcata aaaaagaaga agatactaat gatttagttt tatacaagac
      961 cttatatgat gatacaggaa tatttgcaag acctttggat atgtttttag aaaaagtaga
     1021 tacagataag tatgtcaatt caattcaaga atacagattt gaagaagtat ataaataata
     1081 tgagagaaat atgcttataa atttagaaaa attctttaac agtacaataa tttgcagaaa
     1141 tacaaatgag atttattgta aataatatgt tagattagat tataaaatag aaacaaagta
     1201 aagtattata tgatataatt aagttttgag aagtgataat ttatatttaa taaaaaatag
     1261 gcatatatta ggaggacata tgggaaatac tttttatata gtaagacatg gacaaacgga
     1321 ttggaatata ttgggaaaaa cacaaggtca tggtaattct gaccttacgc ctcaaggtat
     1381 agaacaggca aaagaacttt ctgaagatat tggcaagtat tcaattgatt atatttttag
     1441 tagcgacctt gggagagcaa tgcaaacagc tcaaatttta ggagataagc tcaatattga
     1501 agttcaaaaa actgaggcac ttagagaaat gggattcgga gtttgggaag gccttttaat
     1561 aaaagaaatt caaaaagatt attcagatat atatgcaaca tggagaaatg aaccacactt
     1621 agtaaatata ccagaaggcg aaactcttaa gattataaag gaaagagtag atgcttttat
     1681 aaaggaacta aatgaaaaat atgataataa aaatataatt cttgttacac attcaattac
     1741 attgagggtt atgttgttgt catttttaga atcaggtatg gaaaatatat atagaattaa
     1801 gcaagataat actgctttaa atatagtaga atttaaagat tatggacctg ttatagtaaa
     1861 aatgaatgat acaagtcata taaaaaatca tgtaaagata aataattctg cactggagta
     1921 ggaatcaatt ataaggagaa atttatgagt aaagttatag tagttggtgg aggagcttct
     1981 ggaatgatgg cagctttgtc agcttcaaaa aacaataatg aagtaatatt agttgagaga
     2041 aatggagaat taggaagaaa acttagggca actggtggcg gtagatgcaa ttttaccaat
     2101 aatagagaga tagaagattt ttttgataaa gttgtcagta ataaaaaatt tctctatagt
     2161 agtttctata catttacaaa taaagacctt atttcgtatt ttgaaagtag aaatttggaa
     2221 tataagattg aagaagaaaa tgaccacaaa gtatacacta aaaatgataa atctatagaa
     2281 gtaatagaag tattaaataa agatttactc aatcacaatg taaagataat gtataataag
     2341 aaagttatag atattataac tgaagaaatt gctctaaaag atgattctaa taaggacaag
     2401 tcaaagtatc taataaaagg gattatttta gataatggag ataaaatact aggagacaag
     2461 gttattatat caactggagg agtgagttat tcaaagacgg gttcagatgg ttcaatgtat
     2521 aagattctta aaaagcatgg acatacatta aacaaactat atcctgcttt agttccactt
     2581 acaatagagg aaaaatggat taaagattta caaggaatat ccatgaagaa tgtggaaata
     2641 tcttgtaaga ttaagaaaag gaaaatatca aaatctggag acatgctatt tgcccatttt
     2701 gggataacag ggccttgtgt tcttataatg tcttcttata ttaacaagat tattgaaaag
     2761 gaaaaggtag aattaaatat agatttctta ccaaatttaa gtactgatga aatctctagt
     2821 ataatcagag catttccaaa taaaaatgtt ctaaataatc ttaagcaaat actacctcaa
     2881 aatttcttaa aagagatttt ttctctatta tctttagttg ataaaaaggc aagtgatttg
     2941 tctaaggcag atgaaattag aattattgag tatataaaaa atatgaagct aacttgtaat
     3001 gggactacag gtataaacac tggtatggtt acttctggtg gtatttcagt taaagaaatt
     3061 aattcttcta ctatggaatc taaattagtt aagaacttat tttttacagg tgaagtaatt
     3121 gatatagatg cagaaactgg tggatataat ttacaaatag cattttcaac tggatatctt
     3181 gcgggtataa gtgtataaat ataatttttt aataattaaa tatgaataaa tagaggtgta
     3241 tatatgggta atttagttat agctgtagat ggtccagcag gagctggaaa aagtacaata
     3301 gccaagatag tagcaaaaaa gctaaatata aattatatag atacaggtgc tatgtacaga
     3361 gcagttactt ataagtgttt aaaaagtggt attgatgtca ataatgcaaa agaagttatt
     3421 caaatagctg aaaattcgga tatagatttt aaagataaca atatatattt ggacaacgaa
     3481 gttataaatg aagaaataag aacaatagaa gtaagcaata atgtttctaa tgtagcaaaa
     3541 ataaaagaag taagacagtt gatggttgaa gttcaacgaa aaataggaat gaaaagttca
     3601 gtaatactag atggtagaga tataggttca tatgttttcc cagatgcaga ttacaaattc
     3661 tttttagtag ctactccaga agaaagagga aatagacgct ataaagaact ttgtaataaa
     3721 ggatataata ctactttaga agaagtaatt gaggatataa taagacgaga tgaaattgat
     3781 tcaaatagag aatttgcacc attggtaaaa gctaatgatg cactagaaat agatactaca
     3841 ggaaaaacta tagaagaagt tgtagaagaa gttgtatcta aaataaatct ttagtttttt
     3901 atgcatcata atgaaaataa atgtagtgtt gaagggagat aaacgtgaat ttttatagat
     3961 ttgtaataaa tatttttaag ggtttttcta aaatattttt taaatatgaa gttataggtg
     4021 cagaaaatat tccagataga ggaaatatag taatagcatc aaatcataaa tcgaatcttg
     4081 accctatatt cttggctgct gccatagaaa atagagaaat agctgcaatt gcaaagaaag
     4141 aattgtttaa agtaaaaccc ttgggattta tattaaaaaa attgcatgtt atgcctataa
     4201 atagagaaaa accagatgta tctacaataa aaactatttt aaggtcagtt agagatggtt
     4261 atgtattagg aatatttcca gagggtacta gaataaaagg agattctttt ggtaaggcta
     4321 aggcaggtct ttctgtattt acaataaaaa gtaaatctaa agttgttcca gtatcaataa
     4381 tatctaaata taaacttttt agtaaagtaa ttgtatatat tggagaacca atatcatttg
     4441 aagaacattt taaggaaaaa ttgagtaatg atgaccatga aaggatatca caagagattt
     4501 tagaagtgat aaaacaaaat tactttaaat attcaaaata agggggtaaa gttatgaaca
     4561 taaaaatagc aaaaaatgct ggcttttgtt ttggagtaaa aagagcaatg aaaatggcat
     4621 gggatgaagt tgaaaaaaat gattctggca tatatgcttt aggtcctctt attcataaca
     4681 agcaagcagt agcaaaatat gaagaaaaag gtcttaagac tgtgaatgaa attgatacaa
     4741 taccaaatca tgaaaatatg ataataaggt ctcatggagt tcctgaaaat atatataagg
     4801 aagccaaaga taaaaaatta aaaattgtgg atactacatg tccatttgta aagaaaatac
     4861 atacagtagt aagtgaatat cataataaag gctatgaaat aatagttatt ggagatatga
     4921 agcatccaga agttataggt ataaatggat ggtgtgaaaa ttcagctata ataataaaga
     4981 ccttagaaca aatggaaaat atggaatttg ataactccaa aagatattgt cttgttgcac
     5041 agactacaat aaatccagag ttgtatataa gtatagttaa taaactatca gataaattag
     5101 aagaaatagt atttaatgat acaatttgtt cagctacaaa aacaagacaa gaatcagcta
     5161 aagagttagc taaagaagtt gattgtatga tagttatagg aggtaaacac agttcaaata
     5221 ctcaaaagtt agtaaaggtt tgtgaagact tagtaccaac ttttgcaata gagactaaag
     5281 atgaattgga tgtaaatacg cttaaaaaat ataaaaattt gggtattaca gcaggggcat
     5341 ctacacctaa ttggataata gaagaagttg taacattcct tgaaaattta taatttgata
     5401 ggtaataaaa ataggtggac attgttataa tttatcataa tatccacctt tgatttcata
     5461 ttacatgtgg taaatagata acctaatatc aaaaagtgtg tatcttaaga tgaaaaacta
     5521 atagtataag atgcgcactt tttaatatta gaaattcaaa agaaatgttg atattgatta
     5581 tcaatagaaa ataaaatata atataattgt tgcaaaatta gtctaggagg taaattaatg
     5641 gaaatgttga tatccgcttc tgaagaggcg tttttacatg taggttctat gattggattt
     5701 tttattttgt tatttggata tattaattat aaaactagtg gaaattttac taatatcata
     5761 tcaaaaaaca gaaaatttca accattaata ggtgctttaa ttggagctat accaggttgt
     5821 ggcggaagct tggcaataat gccattgtat ataaatggaa aattaagctt tggagcaata
     5881 attgcaagtt taatagctag tatgggagat gcagcatttg tacttatttc atcaaatatt
     5941 aaaatgtatt tttttgtcac tatagttagt acaattacag gaataataac tggacaatta
     6001 gtggattact ttaaactaga agaaaaatta gggttaaaaa accgtagaaa atcagataaa
     6061 tacgatataa gtaaaaataa ctctaaagaa aataaagaac aacataatca tgatgaaata
     6121 attttagaca ctctagctaa atctcatgga aatacaaata gacttgcatt tataataaca
     6181 catggaaagg gatataaaat ttatatagga attatagtaa tcggttttat atttatgtca
     6241 cttgctcact ctggacttaa tcttcctata attgagaagt tacattcatt ggaagaggta
     6301 atagcagtga ttggaatatt attttcaata atttatatgt ggtgttttaa aaaggtattt
     6361 aaaaattcta atcaaaaaga ggaagaaaat aaaaagattt ctttgcgaga aatgcttatc
     6421 cattctgttg gagaaatatc atttgtaatt acatggattt ttattgcata tcttatttac
     6481 gatttgataa tattaatttt aggtggagat gaatacttag ttaaattagt attatcaaca
     6541 ggtgtaataa gtgtatttat aggtgcaggt cttggactta ttccaggttg tgggatacaa
     6601 attgtgctta tgtcatttta tttgaaggga actataccac ttggtgcagt gatagccaac
     6661 tctatttctc aagatggaga tgcacttttc ccacttttag caatggataa aaaatcttct
     6721 ttatgggcta tgattattac tactatacca gcaatacttg taggtatgat agtttatgca
     6781 tttttaggtt aaatataatt atgaataaag aagtattaaa aaaataataa agcacataaa
     6841 tttatttata agaatagtga agattaaata taaaaataaa aaattcaata tgaattaaat
     6901 tattgcttaa atattaatga gaacatggga gataatatta ttcctcatgt tctctctatt
     6961 taattaaagg aattatggaa caaatttcaa gtgatacttt ctatcaaaac tcatatataa
     7021 tatatattaa taaattaaac tataatacgt aatacagcca gtatattgat ataaaaaact
     7081 taggaggagt tgtaaaaatg ggtaatttaa aaagacagtg tgatgtttca agtggaagag
     7141 aaaaagctga cattgtacta aaaaatggga caatcataaa tgtatttaca gaagagttaa
     7201 taacaggaga tgtagcaata gtaggagaca ctatcgttgg cattggcgat tacaagggca
     7261 atgtagaaat tgattgtagt aacaaatata tatcacctgg ctttattgat gcacatatgc
     7321 atattgaatc tacaatggta atgccaattg aattatcgaa aaaattatta aaatcaggta
     7381 cgacaactat catagccgac ccacatgagt tggtcaatgt aaaaggagct agtgcaatag
     7441 attttttatt agaatcgaca aaagatatac cacttaatgt atatattatg gtgccttcat
     7501 ccgtacctgc aacaagtttt gaaactaatg gagttggtaa atttagtgca aaagacatgg
     7561 agagttatgt aaataatcct agaattttag gtttaggaga ggtaatgtgt tttaatgatg
     7621 ttataaattc tgaaaatgaa atcttggata aactagagct ttttaaaaat aaagttgtag
     7681 atggacatgc acctaatata aatggtaaat cacttcagac atatgtatgt gcaggaatag
     7741 aaaatgacca tgaatgtata acttttgatg aagtatatga aaaattgaga gcaggattaa
     7801 aaattttaat aagagaagga agcgcagcaa aaaatttaaa gtctatagta agtggtatgc
     7861 taaagcataa cttgccaatt gaagaattta tgttttgcac agatgataag cacttagatg
     7921 atatagaaaa acaaggtcac ataagatgga atataaaatg tgcgattgat ttaggtatgg
     7981 aacctgttag ggctataaaa gttgcaactt ataattctgc aaaagcatat gggttaagga
     8041 aaattggagc aataggtgcg ggttataagg cagatattgt tgtattgaat gatttagaca
     8101 aaatggaagt ggatagtgta tataaagatg gtaatttggt aaacgaagag atgttttcta
     8161 attacaatta tgagattaaa gataaagaat tgcttaatac agtcaagttt aaatatatta
     8221 ataaagaaaa aatacagtta aaagtaagtg aaaaaaatta tgtaatggag attgtacctt
     8281 atcaaatttt gacaaataag gtatatgaat ctttaccatg tgcagatgga tattttgttc
     8341 caaataaaga gtattcaaaa ctttgtgttg ttgagagaca tagaatgact ggaaatgtag
     8401 caattgctcc attaaaagga tttggcataa aaaatggagc aattgctact actgtggccc
     8461 atgattctca taatattata gttgcagggg ataatgatga tgatattcta gtagctataa
     8521 attacttaaa ggaaatacaa ggtggttatg taattgtatc aaatggaaaa gttcttgcac
     8581 acctaagtct tcaagtagct ggattaatta gtacttttac agctgaagaa gtacaagaaa
     8641 ttactgataa tatgttggag atagcaagaa aaatgggagt tccagagtat gtagaccctt
     8701 ttattacact ttcttttatg gcacttccag ttgttccaca aataagactt acagatttag
     8761 gtctatttga tgttgaggaa tttaaattta tatagataat ataaaagtaa taataaatga
     8821 ggctttctca aaatagggat aagtctcttt ttattgttga aatgatgaaa atatatttga
     8881 tttcatacaa aaaagagcat ctcatgaact aaaaatttta tttttagata tccttatttt
     8941 aattaatcta tcatttaacc ttgttttaag catagatatc ttaataaaac tattttcagt
     9001 ctattttaac atataaatac atgaaaatta tatatttatg aaactttata cataaaaaat
     9061 aaaatatttt atttatatat agatttatag ggtaataata taagcatagt ataaaattat
     9121 taataaaaac aaaaggagag atatttatgt tgagtatagg aacaaaagca ccagaattta
     9181 cactagaaga taaagatgga aataaggtaa gtatgtcaga ttttaaaggt aaaaaagtag
     9241 tagtatattt ttatcctaaa gataatacac caggatgtac acgtcaagct tgtgctttta
     9301 gaaatgcata tgatggattt aaaaaggaag atatacaagt tattgggata agtaaagata
     9361 gtataaaatc acatcaaaaa tttgcagaaa agcatgaact tccttttatt ttactttctg
     9421 accctgactt agttgctata aaagcttttg atgtatggaa agagaaaaaa atgtatggaa
     9481 aaactgcatt gggagttgta cgtgcgacat atattataga tgaaaatgga attattgaaa
     9541 aagtctttga aaaagcaaaa ccagacacaa atgcacaaga gattcttgaa tatttggaaa
     9601 aacaggagta ataagaattt atgattataa tgttatcacc agctaaaaat atgaagaata
     9661 tagaagtatt tgatagagat ttgagtttgc cttgctttat agataacaca aaagaaatag
     9721 tggaaaatat aaagacattt ggtatagaag attttaaaaa taaaatgaaa attaatgaaa
     9781 aattagctgt attaaataaa aatagatttg aaagcattaa atttgataga ttaggaaacc
     9841 ctgcaatctt aacttatgat ggaatacaat ataaaaatat agaagcagaa aattttacaa
     9901 ggaaagatga agagtttgca aatagctgta ttcgaatcat aagtggattg tatggtgttg
     9961 taaaaccata tgatagtata tatgaatata gattagaaat gcagacaaaa ttacaagtag
    10021 gtgagtttaa gaatttatac gaatattggg gaaatcgtat atataaagaa ctaatcaaag
    10081 aaaaaacagc tatcgttaat ctttcatcaa atgaatacag caaatccata gaaaaattta
    10141 ttaaagatag tgatacatat attacatgta cttttaaagt aaataaaaat ggcatattga
    10201 aagtggaatc tacacaagca aagaaagcta gaggtatgat gacaaaatac attgtaaaaa
    10261 ataggataag agatattgaa gaactgaaaa agtttgattt agaaggttat aagtataaag
    10321 aaaacttatc taacaatagt gaatacatat ttgtaaaaga gtagaagttt tgttaaatat
    10381 taaatggcat tgttaaattt aggtaaaata tacttagtgt gtattgaatt gaacttgatt
    10441 ttaaaatata attattctta aattaaaaaa agaaaggagt ggtcttccaa aatggaaaat
    10501 cacaaacaga tgccttggca ttcaagagca acgcatgaaa ttttagaaag tctagattca
    10561 tcagaggagg ggctcaccga taccgaggcg gaaaaacggc ttaaagagaa tggcttaaat
    10621 gaacttagaa aaaaaccacc tagaacaact ttacaaatgc tttggagtca aattacagat
    10681 gctatggtta tgatactaat aggagcagca atattatctt tgatatttgg cgaatttaca
    10741 gaagcttttg taatactaat aattgtaaca gtaaatgcag ttattggaat tgttcaagag
    10801 aaaaaagcag aatcatcatt agaagcatta aaaaatatga attcacctac agctcgtgtt
    10861 atgcgtgatg gtgaagaaaa tgttattcca gcaagtaatc ttgtagtagg agatattgtt
    10921 tttttagaag atggagctat ggttccagca gaccttcgtt taatagaaac atcaagttta
    10981 aaaattcaag aggcttcttt aacaggagaa tctgttccat cagaaaaaga tgcagataca
    11041 atcttaccta aagaatgtgt attaggagac cgtagcaata tggcgtatac atcttcaata
    11101 gtcacatatg gtagaggtgt aggtgttgta gtagcaactg gtatggatac agaagtgggt
    11161 aacatagcac atcttttaga ttcacaagat gattttgata ctcctataaa acgtaaactt
    11221 aatactgttg gaaagacatt aagtgtcata ggtattattg tatgcgttgt tatatttgca
    11281 ataggtgcat tttatcagag acctctcttg ccaatgttta tgattgcaat ttcccttgcc
    11341 atatcaatta ttccagaggg attacctgca acagctacta ttgtaatggc tttaggcgta
    11401 cagcgtatgg ctaaaagaaa tgctttaatt agaaaattac ctgctgttga aacattagga
    11461 ggagctacag taatatgttg tgataaaact ggtacattaa ccttaaataa aatgactgtt
    11521 acacatattg ctgtcaatgg agattttgaa tctggaaaag tgacatcaat agatactgca
    11581 tctgaaaaac atccattagt gtataaagag attgtttatg ctggtgcact ttgtaatgat
    11641 gcatgtatta atccagataa aacagatgaa atattaggtg acccaacaga gggcgcatta
    11701 atttttatgg ccaaaaaatt tggaatagat caagaagctc ttgaagaaga atatccacgt
    11761 gtatttgaac agccttttga ttcagataga aaatgtatga caactgttca taatatagat
    11821 gaaaagatta ttgcttatac taaaggagca gtagacgaaa ttcttgacct atgtactaaa
    11881 atattaactt caaaaggtga aagaaacatt accgaagtgg ataaaaagaa tatacacgaa
    11941 ttatgtctta acatgtctaa agatgcattg cgtgtacttg gttttgctaa gcgtgagatt
    12001 tcatctatac caaaagaaga tagtgaaaat atagaatatg atttaacatt tataggtatg
    12061 gttggtatga ttgacccacc aagaactgag gtaattgatg cagtaagtac ttgtaaagaa
    12121 gcaggtataa gaaccataat gataacaggt gaccataaag ttactgccac aacaatcgct
    12181 catgagttag gaatatggag tgaagaaaat actgtgatat ctggtgatga acttgataat
    12241 ttgagtgatg atgaactaga tgaagctgtt aaaaatacta cagtatttgc tcgtgtatcc
    12301 ccatttgata aacttagaat tatacaatct ctaaaaagaa ttggagaagt accagcaatg
    12361 actggggatg gtgtaaatga ctctcctgcc ttaaagtctg ctgatattgg tatagccatg
    12421 ggtataagtg gtacagatgt tgcaaaagat tcttctgaca tgattttaat ggacgatagt
    12481 tttacaacaa ttgcttatgc aataaaagaa ggtagacgtg tatatagaaa catacaaaaa
    12541 gttatacaat ttttgcttgt tggtaatata gcagagatac ttacgttgtt tgtagcaata
    12601 ttattaaatt gggatactcc gcttcttgct gttcatattc tatgggtaaa ccttgcaact
    12661 gctacgcttc cagcccttgc tcttggagtt gacccaccaa gcagaaatat aatgaagcac
    12721 cagccagtga aatcaaccac tttgtttgaa aaaaatctta tacaaagagt tgttacccaa
    12781 ggtatatttg tggcaataat gactatttta gcatataaaa ttggtcttaa tatagaaaat
    12841 cattctgtag gtcaaacaat ggcattttgt gtattagcat tttcacagat gttacgagca
    12901 tttagtcagc gttcaaatac agattcaatg tttaatagaa ataatggtaa aaatccattt
    12961 cttttaatct catttttaac ttctgcattt ttaatagcag taatactatt tgtacctgtt
    13021 tttagaaatg cttttaactt aacagtttta aataacttag agtggcttat aacgattgtt
    13081 ttagccataa tgtctgtaat tcaagtagag gttgggaaaa ttattaaaag aaaaaaatta
    13141 aaaaaatagt tgacaaaatt aggaaaaata tataagatat aaaacataga aaaattaaat
    13201 aaatcttatc cagagaggtg gagggactgg ccctatgaaa cccggcaacc agtatattta
    13261 tatacattgg tgctaaatcc tgcggcgaaa gctgagagat gagtataatt aagcttatac
    13321 agagcctgag ttatatactc aggctttatt tattttaact ctcatctggt aaatagttta
    13381 atttttttta ctaaaatcta agggggatac aagaatgtat aataaagaaa caatatgtgt
    13441 gcaaggaaat tataaaccag gtaatggaga accaagagta ctacctttat atcaaagtac
    13501 aacttttaaa tatagcagta tagaccaact tgctgaatta tttgatttaa aagttgatgg
    13561 acatatatat tcaagaataa gcaatcctac tattcaagct tttgaagaaa aaataagttt
    13621 actagagggt ggagtatctt ctgtagctgt atcatcaggg caatctgcaa atatgttggc
    13681 agttttaaat atatgtaaat caggagatag tatactttgt tcttcaaaag tatatggagg
    13741 aacattcaat ttactaggac ctagtcttaa aaaatttggt atagatttaa tatcgtttga
    13801 cttagattca agtgaagatg agatagtaga acttgcaaag gaaaatacta aggttgtgtt
    13861 tgcagaaaca cttgcaaatc caactcttga agtcatagat tttgaaaaaa tagcaaatgt
    13921 agctaagaga attaatgttc catttattgt tgataattca ttagcatctc cagtgctttg
    13981 taacccttta aagtatggag caaatatagt tactcattct accacaaaat atttagatgg
    14041 gcatgcttca agtgttggag gaattatagt ggatggtgga aactttaact gggataatgg
    14101 aaaatttcca gaattagttg agccagaccc aacatatcat ggtataagct atactcaaaa
    14161 atttggaaat gccgcatatg caactaaagc aagagttcag ttgcttagag actatggaaa
    14221 ttgtttaagc ccattcaatg catatcttac taatttaaat gttgaaacac tacatcttag
    14281 aatggagaga catagtgaaa atgcacttaa aatagctaga tttttagaaa aacatgaaaa
    14341 tgtagattgg attaattacc caggacttga agataacaag tattatgaga atgccaaaaa
    14401 gtatttatca agaggatgta gtggtgtttt atcatttgga gtaagaggtg ggttagaaaa
    14461 tgccaaaaaa tttgtggaaa aattacagat agcatctttg gttacacatg tttcagatgt
    14521 aagaacttgt gttatacatc cagcttcaac tactcataga caattaacag aagaacaatt
    14581 aattgcatct ggagtattgc cttcactaat aagattatct gttggaatag aaaatgtaga
    14641 ggatttaata gctgatttaa atcaagcttt aaatttctaa tattctaaga gaaagagaag
    14701 tgaaaagata tggctttaat actaccaaag ggattacctg ttattaataa attattagat
    14761 gaaggaatag atgtaattta taaagaagat tttaaaaagg aattaaaata tgaagaaaac
    14821 atagacacta agatagctat actaaatcta atgcctataa agatagatac agaattagat
    14881 ttactaagaa ggatagataa aacaggattc aacgtgtctg tggaatttat taaaatttca
    14941 acaagagaaa gtaaaagaag ttgtaatgaa tatgttaaag aattttataa aacatttgat
    15001 gaagccaaag gagagtattt tgatggcttt ataataacag gagctccagt tgaacaaatg
    15061 gagtttgaag aagttgatta ttggaatgaa ctagaagaaa ttatggatta ttctaagaga
    15121 aaaactaaat ctacattata tatatgttgg gcagctcaag caagtcttta caaatactac
    15181 aatgtaaaaa aacttccact aagtcaaaaa tgttttggag tatttaagca taaggtagat
    15241 aaagatagta aaatagtaga tggatttgaa aatgaatttt ttgctccaca ttcaagacat
    15301 actactgtaa atattgaagc tttgaaagag aataaagagt tgagcattgt gagtcattct
    15361 aaagaagctg gtccctatat aattacaaat tcaagagatg tgttcgtaat gggtcatagc
    15421 gagtatgaca aatacactct tgataaagaa tataagaggg atataaataa aggagataaa
    15481 atatctatac ctcaaaatta ctatattaat gatgaccctt cagaagagcc aactgtaaaa
    15541 tggaaaaaac attcagaatt gttgtttaga aattggataa aaaactattt gatacaataa
    15601 attttaagag tatataaatg ttaactaaaa aaatatagaa atttataggt ataaagttaa
    15661 tcttaatgat actttatacc ttattttttt aaaatttgac cttataatag tagttttata
    15721 ttttaagaaa atcactttag gtgtatgtag atagaggtgg ctttaatttt atatatggtg
    15781 cgataatggg agactaaacc tttatagtat caataaattt gtattttaac ttttaaaaga
    15841 gattaaaaag ggagaaatca gtgtaaaata tatatataga ttttttatta aattttatta
    15901 aaaatatcct gtataaaatt aaatatcaat aagataacaa aatttcaatt gaagttacaa
    15961 tattgtaatt gacaataaat acttaatact ataatattta atacattaaa cattatagtg
    16021 ttaagtattt atttacagta tgcacttcag cctaaatttg aaataaagta ttttcgtagg
    16081 gagcgtttgt atggatgaga tgagttttaa gaaagagcta ctagagttga ctagagatat
    16141 aaatatgaaa tttacaactt tattaagtga tttttatcag ccacttggta ttacagcagt
    16201 gcaagctttg attttatctg agttatgtga acatggagaa aaaaaaataa gcgatttagg
    16261 gaagaactta aacatgacaa atagtaatgt atcagtaatc tgtcaaaggc ttgaaaaaaa
    16321 tggcttttta aatcgcataa gagatataga agaccaacga attgtaaaag taaaagttac
    16381 aaataaatca ttggatatac aagaacatat ttcaagtagt atttttgata gttattttga
    16441 aaatatgacc agtgaaaaat tgcaagatat ggaagatata atagaaggtt tagagaaatt
    16501 aaataaactt ttaacttaca ttgattgcaa ataacataaa aataatttaa aggagaataa
    16561 aattatggat gaacataaac cccaaaaaag gtcagcactc tattattata cagttataat
    16621 aatagtagta atgtttttaa atgctttaat atttccaagt ttaatggata aaaaaatggt
    16681 tgagattgac tatggaaagt ttctcacaat gttggataca ggaaaggtta aatctgttga
    16741 gatagaaaat aatagaatag caataaatcc atctagtagc aaggataaga atatatacat
    16801 aactggtaga atggatgacc cagaattggt agatagacta aaaaatgcaa aagtagaatt
    16861 tactaaggtt attcctaagg aaaattcacc tcttttaagt attttactta catggataat
    16921 acctataggt attcttatgc tatttggtaa cttaatgatg aagtctatgc aaaagcgaat
    16981 gggtggtaat gctatgcaat ttggaaaaag caacgccaaa atgtatgtat ctgctcaaag
    17041 tggaaaaaga tttaatgatg tagctggtca agatgaggca aaagaagctt taactgaaat
    17101 agtagatttc ttacacaatc cagaaaagta taaaaagata ggagcacaaa tgcctaaagg
    17161 agcactttta gtaggacctc caggtacagg aaaaacactc cttgcaaaag ctgtagcagg
    17221 tgaagcaaat gtaccatttt tctcaatatc tggttctgag tttgttgaga tgtttgtagg
    17281 aatgggagct tctaggattc gtgatttatt taaacaagca aaagaaaaag ctccatgtat
    17341 agtgtttata gatgagattg atactattgg taaaaagcgt gataatggaa gtggattagg
    17401 tggaaatgat gaacgtgaac aaactttaaa tcagttgtta agtgagatgg atggttttga
    17461 tggtggaatt ggagtagtaa tacttgctgc tacaaataga cctgacagtc ttgacaaagc
    17521 attacttcgt ccaggaagat ttgatagacg tataccagtt gaacttccag accttggagg
    17581 acgtgaagct atattaaagg tacatgctca aaatgtaaat gtaagctcag atatagatta
    17641 taatgcaatt gcaaggtcta catcaggagc atcaggtgca gaacttgcaa atattgtaaa
    17701 cgaagcagca cttttagcag ttaagtctgg cagagatata gtagaacaac aggattttga
    17761 tgaatctgtt gaaactgtaa ttgctgggta tcaacgtaaa ggagctgtaa tttcagataa
    17821 ggaaaagaaa attatagcat atcatgaaat aggtcatgca cttgttgctg ctatgcaaaa
    17881 acattctgca cctgtccata agattactat aatacctaga acttcaggag cattgggata
    17941 tactatgcaa gttgctgaag atgagagtgt acttatgtct aaagaagaag caattgataa
    18001 aataactact tttacaggtg gtcgtgctgc tgaagaggtt atctttaaca cttgtacatc
    18061 aggagcatca aatgatatag aacaagctac taaaattgca agagctatgg ttactcgcct
    18121 tggtatgagt gaagagtttg atatggtcgc tcttgaaact gtaagcaatc aatatttagg
    18181 aggagatact tctcttgctt gttcttccga cactgctgct aaaatagatg aagaggtatt
    18241 taaaatagta aaggcttgtc acaaaaatgc aattgaaatc ttagaaaata atattgataa
    18301 attgcatgaa cttgctaatt atttattaga aaaagaaact attacaggtg atgagtttat
    18361 ggaaatactt aacttaaact aaaatgatta tacacaagat aaaaatgcct attgttaggc
    18421 atttttattt tacaactaaa ttttttaaat tcatatttaa atctttacat aatcttaatc
    18481 attaaaaata catcttaact acaattttat atcggaattg atattataat agtaatgaat
    18541 ggtgtaagga ggttttttaa tggatagacc taatcctgat attctattag aaaaaattaa
    18601 aaatgaagaa gaaaaactaa gtagaggtca attaaaaata ttctttggtt atgcagctgg
    18661 agtaggaaaa acctactcta tgttggaatc tgcacaaaat ttaaaaaaag tgggtgtaga
    18721 tgtagtagtt ggatatattg aaccacacac tagaccagaa acattggctt tacttgatgg
    18781 cttggaaatt cttcctgtaa aggagataga atataaatct ataaaactaa aagaatttga
    18841 tttagatgct gctttagcaa gaaaaccaga agttatatta gtagatgaat ttgcccattc
    18901 taatgttgct gggttaaggc atacaaaaag atggcaagat atagaagaat tgctgttagc
    18961 aggcataaat gtctacacta ctgtaaatgt acaacattta gaaagtctaa atgacattgt
    19021 tgagataatt acaaacgtat cagttaaaga gactatacca gataagtttt tggatacaaa
    19081 tacacagtta gaacttatag atgttgaacc tgatgtactt cttgaaaggt tcaatgaagg
    19141 taagatatat aagaaagaac aagctactag agcaaaaaat aattttttta taaaagataa
    19201 cctggttgca cttagagaaa tcgcacttag aaaaactgca gagagagtta ataaagaagt
    19261 tcaaatgacc agactatcaa agggtgatgt aactgtaata cctacatcag atacacttct
    19321 tgcatgtata agtccatctc catcttcagc aaaagttatt agaactgcat ccagaatatc
    19381 agacagtaca tttgcaaaat ggatagcttt atatgttgaa actccaaaca cagcaagact
    19441 tccatttgat gaacaaaaac aactacaaaa taacctaaaa ttagctaaga aattaggtgg
    19501 agaaataatt gttttacatg gtgaaaatat aatagagcaa atacttagaa ttgcaaagct
    19561 gagaaatgta acaaaaattg taataggtag aaatcattca agtaataaaa gatttagtaa
    19621 aagatttaaa aaagatatag ttgacaaatt aattgatgaa gttgattata tagatataca
    19681 tataattcca tataaatttg cctcagatgt taaatacaaa cctaaaaagg ataaatcatc
    19741 aataaaatct aaatttaaaa ttagcaaagt tgattttata aaacttatat ttattacttt
    19801 gatgataagt attttagcat atgcagtaca aagcatggga tttataagag aaaatatatt
    19861 gttaatatat atgttgggtg tagttttagt ttctat
//
LOCUS       DAKBNN010000058        19667 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000058 DAKBNN010000000
VERSION     DAKBNN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.058"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            366..1643
                     /gene="hflX"
                     /locus_tag="OUB02_001750"
     CDS             366..1643
                     /gene="hflX"
                     /locus_tag="OUB02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCT8885511.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1876..2373
                     /locus_tag="OUB02_001751"
     CDS             1876..2373
                     /locus_tag="OUB02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8885512.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2423..2947
                     /locus_tag="OUB02_001752"
     CDS             2423..2947
                     /locus_tag="OUB02_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCT8885513.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            3259..4623
                     /locus_tag="OUB02_001753"
     CDS             3259..4623
                     /locus_tag="OUB02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCT8885514.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKIVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEECEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQKHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            4762..5928
                     /locus_tag="OUB02_001754"
     CDS             4762..5928
                     /locus_tag="OUB02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT8885515.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            6045..6827
                     /locus_tag="OUB02_001755"
     CDS             6045..6827
                     /locus_tag="OUB02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCT8885516.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(6946..7599)
                     /locus_tag="OUB02_001756"
     CDS             complement(6946..7599)
                     /locus_tag="OUB02_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8885517.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(7623..8987)
                     /locus_tag="OUB02_001757"
     CDS             complement(7623..8987)
                     /locus_tag="OUB02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HCT8885518.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSIFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            9645..11075
                     /locus_tag="OUB02_001758"
     CDS             9645..11075
                     /locus_tag="OUB02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8885519.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            11178..12281
                     /locus_tag="OUB02_001759"
     CDS             11178..12281
                     /locus_tag="OUB02_001759"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCT8885520.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            12296..12532
                     /locus_tag="OUB02_001760"
     CDS             12296..12532
                     /locus_tag="OUB02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8885521.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            12549..13598
                     /gene="vorB"
                     /locus_tag="OUB02_001761"
     CDS             12549..13598
                     /gene="vorB"
                     /locus_tag="OUB02_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HCT8885522.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            13600..14340
                     /locus_tag="OUB02_001762"
     CDS             13600..14340
                     /locus_tag="OUB02_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HCT8885523.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            14343..14882
                     /locus_tag="OUB02_001763"
     CDS             14343..14882
                     /locus_tag="OUB02_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HCT8885524.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            15006..17105
                     /locus_tag="OUB02_001764"
     CDS             15006..17105
                     /locus_tag="OUB02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HCT8885525.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAVVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(17372..17887)
                     /locus_tag="OUB02_001765"
     CDS             complement(17372..17887)
                     /locus_tag="OUB02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HCT8885526.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLASVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(17979..19190)
                     /locus_tag="OUB02_001766"
     CDS             complement(17979..19190)
                     /locus_tag="OUB02_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HCT8885527.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         7563 a         2281 c         3354 g         6469 t
ORIGIN      
        1 ctacttaaat ttaagaatag ggttaaatat aaaatgataa ttacatataa gttaattaaa
       61 ctaatgtatt tttaatatat atttagaatt ttaatccttc ttatgatata agtagcttgt
      121 tttgtattac ttagatttgt aaggagtttt tttattgaaa aaactgcata atatatttgg
      181 gaggaggtgt aagaaagtga ttttttatat aagattgaga aaataatgca cgattatggt
      241 tattataata gtaaataaga agtgtcattt aaggataaga taaaaagcaa aattcttgat
      301 gaatttaatt atagagccat aatctcgtgc tatagattaa ataaaaaata gcagataagg
      361 agaatatgga tatggcgaaa aaaggaataa cagtaggatt aaacataaat aataaatctg
      421 aggactttaa tgaactaatg attgaacttg aaaacttatg ttcagcatgt gatattgatg
      481 ttgtagggag tataactcaa aatgcaaagc aggttaatag agcattttac attgggacag
      541 gtaaagttga ggaaatatta aacttaataa agaaagaaaa tatagagata gttatatttt
      601 acaatgaact ttctacatca caactaaaaa atcttgaaga aaagcttaat tgtgaaatta
      661 tagatagaac ggcattgata cttgatatat ttgcacaaag ggcaaaaact agagaagcta
      721 aacttcaagt agaagtagca agtttaaaat atatgttacc aagacttata ggttcaaatg
      781 aaaatttagg acgtcaaagt ggtggagttg gtactaaaaa tagaggttct ggtgagaaaa
      841 aattagaact tgaccgtaga agaatagaag aaaagataac atctttaaat agggagttgg
      901 atgatttaaa attccaaaga gaaactcaaa gaagtatgag aagaaaatca aatcttccaa
      961 atgtagctct agtcgggtat acaaatgctg gtaaatcttc aataatgaat aaactagtag
     1021 atatatttaa aaactctgaa gaaaagaaag tgtttgagaa aaacatgtta tttgctacac
     1081 ttgaaacttc tgtcagaaat atagttcttg caaataacaa agaattttta ttatctgata
     1141 ctgttggttt tgtaagtaat ctaccacatg acttagtaaa agcatttagg tcaacattag
     1201 aagaagcatg tgaggcagac gtactgttac atgttattga tatttcaaat ccaagctata
     1261 aaagtcatat taaagtgaca gaagatactt taaaacaaat aggtgcagat ggtataccta
     1321 tgatacatgt gtataataaa attgatttaa tagatgtaga agtattggat agaattttag
     1381 atagtatcga taaagaaggt atatttgttt cagttaaaaa agatataaat atagataaaa
     1441 tgataaaatg tatttgtgat agcatattta aagattatgt aagatgtaag ttcttaattc
     1501 catatgataa aggtcatgta gtttcttatt ttaacgaaaa tacgagcatt ataaatacag
     1561 aatacagaga agatggagct attttagatg tagaatgtag caatatagaa tataataagt
     1621 ataaaaagta tgctttagag taaattttga aaaacttatt atagcctaaa atatatttaa
     1681 tttataattt attataaatt gattgtaaat atgattttat gaaacggtat agccataatg
     1741 ctatgccgtt ttgccattta taataaaaat aaatttattg acacaatagt aactaattac
     1801 tataatataa agtaactaat tactatttaa aattatcaaa aaattaaaat ctaagataaa
     1861 gagaagtgta taataatgga tgcagaaagt ttaatgaaaa aatatatggt agatgatgat
     1921 agtagaaaac aagctatatt tgcaagtata tttgtaatgc aaaataaact tcaaactaca
     1981 tgtgataaat tagacccaga attaacaatg aaacaatggt tacttttagc tattgcggac
     2041 tctgtagatg aaactcttac attaaccaaa cttggagaat taatggggtg ttcaagacaa
     2101 aatgtaaaaa aactagctat tgctttagaa aagaaaggtt ttatagaaat tgagcaaaat
     2161 gaaaaagatt caagagctat ctgtcttgtg acaaaagata gagtggaaga gtattctaaa
     2221 aaagtaagag gtatgcagga aaaagtatta cagcttttat ttgaagattt tacagagaaa
     2281 gaagtggaac agttattttt tggaattatg aaattaagta taggaattga aaatgtgaaa
     2341 aattatgtag aaaataatga cgttggagaa taagcaagta tcaaatcaac tttgtatgac
     2401 atcagagagg ggggtaattt atatgaaaaa tgtagtagtg atttataaga gtaagtatgg
     2461 ttcaacaaaa aaatatgcta aatggctttc agaattattg tcttgtgata tttttgaaac
     2521 taagaatatt aatatagaaa aggttatgaa gtatgatacg attattttag gaggaggtct
     2581 ttatgcttgt aatatagcgg gaatttcttt cttaaaaaaa aattataaag aattgcagaa
     2641 aaaaaattta gtagtatttg gcgtaggagc tgctccatat gatgatgaag taataaagaa
     2701 attaagagaa gtaaatttta aggatgaatt aaacaaaatt ccttgctttt attgtcgtgg
     2761 ggcatgggat gagacaaaaa tgtcttttat agattatact ctttgtagtt tttttaaaaa
     2821 atctatagct aaaaaagaac gtcaaggtct taaaccagca gaagcacctt tattgtctgg
     2881 agaagcattt gattggtgta ataaagaaaa tttaactcca atacttgatt atataagaga
     2941 actttaagag gtatatattt ataattattt attatctaaa gttagatttc atagaaactt
     3001 actagacatt atttataaaa taatattatt aaagatggat aatttaattt tttcaataag
     3061 gttataattt taaaaaatgt ttattttaaa ataaattatt ttataagctt acttatactt
     3121 aataaggggc tttttttatt ggaagattta tgttatattg agatgaattt attatgatat
     3181 aaaaaataat gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta
     3241 aagaaaggat ttttaacaat ggatataaaa gagttggtta aaatgcaaag aaaatattat
     3301 aatacaggta aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta
     3361 gttgtatcac aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg
     3421 gattttgaag gttttatgac agaggtaggc atgttttata gtgaattaaa ctttgctata
     3481 aagaatataa gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca
     3541 tctatatcca aaattgttcc acaaccttat ggtgttactc tcataatgtc accttggaat
     3601 tatccatttc aattagcact tataccatta gtttggtcat tagcagcagg gaattgtgtt
     3661 atattaaaac cttctgaata ttctgtgtca acatctagtg tagttaaaga catagttgaa
     3721 gatacttttt caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga atgtgaaaag
     3781 ttactattgg aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata
     3841 atcatgaaat cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt
     3901 ccttgtatca ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga
     3961 aaactaataa atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat
     4021 agaaaaaatg agttaataga gaaaataaaa tattatacta ataagtattt tggggataat
     4081 gcatgtaata atgagcaatt tcctaaaatc ataaatcaga aacattttaa tcggatatta
     4141 tctcttattg ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag
     4201 atagagccaa ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata
     4261 tttgggccta tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt
     4321 atacaaatgc caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat
     4381 aaacttttag aaatgattcc agcaggagga tgttgtatca atgatacagt tactcatata
     4441 gctacgaatt acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga
     4501 aaagctggat tcgatacatt tactcattat aagagtgtgt taaaaaaatt aaatttggat
     4561 gtccctatta gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg
     4621 taggttatat tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat
     4681 tttattttta atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta
     4741 tatagaaata aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca
     4801 atttatacat ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt
     4861 gatgtatttt ctaataattt agatgctata tttacaacat tacaaccttt tataatagga
     4921 tttgctattg catatcttct taattttatt ttaaagtttt atgaagacag aatttttata
     4981 aaaagtgaga aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta
     5041 acatatgcaa cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta
     5101 attgagagta ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag
     5161 cttattgata aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt
     5221 gataagtgga atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata
     5281 ttaggaagta tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt
     5341 gtttcagtat acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact
     5401 tatgcagtat ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac
     5461 tatacatttg gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta
     5521 acatttgtaa tacttacatt agttaaaatg ccatatacat tattaatatc agttataatt
     5581 ggtattacaa atattatccc tttctttgga cctttatttg gagctatacc atctacgcta
     5641 atagtgatgt ttgtatctcc tataaaagct ttctggttat tgttaattat attaataata
     5701 caacagatag atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct
     5761 gcattttgga ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg
     5821 gttataggag ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt
     5881 aaattggaca aaaagggatt accgacagat acttccgatt atatgtagct aaaaaatatt
     5941 atgtataatt aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct
     6001 tggtgctttt tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat
     6061 gtaaatttaa atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag
     6121 caaatggaaa agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa
     6181 cattatactt atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg
     6241 tagagcatta tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt
     6301 tttatactga tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta
     6361 aatttgttag agctatattt cctttatcag ataaaaggga agataacttt gtagctggta
     6421 tgtctatggg gggatatgga gctttaaaat tagcgctttc aaaaccaaaa gagttttcgg
     6481 cagttgcaag tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag
     6541 aaaccaatgc agacattcta tttcacgatt ttggtagacc agaagacgct agaaatagtg
     6601 agaatgattt actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa
     6661 tttatcaatt ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat
     6721 atgcagattc tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc
     6781 gtctttggaa tttatggata aagaaattta tagatataat tggatagtac acttatggta
     6841 ttaacaggta tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa
     6901 ttatgatttt aaaatttcag tctctaagaa tttaataaaa ttttattaag ttcttacacg
     6961 agttattatt ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa
     7021 aatctcttcg cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg
     7081 ctttattaca gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc
     7141 aggtttcata gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc
     7201 tttatcatta acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat
     7261 agcttcttca taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa
     7321 acaaaggtca gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt
     7381 atagattaaa gactgcattt catttcttat atcttcatat attatagctc ctatttcgct
     7441 tttacttgag aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg
     7501 cctagttgtt gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat
     7561 actagctttt gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct
     7621 tttcaatctt tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata
     7681 ggaaccaaaa ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata
     7741 acacttacta taacagcata gatagctcct ttttttgtag cacctttcca gcaaagacca
     7801 attacaaatg gtgcaaatat tacacctgta cataatggag ctggccaaaa caataattct
     7861 aataaactat caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca
     7921 atttgagaat actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat
     7981 ttgttataaa tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta
     8041 gacattatgg cagccaatat agcagctaag aataatacag atattaaagg tggtaaagta
     8101 gtcgatatca tttgaggtaa aacctgaaca gatgatatat ttggaaataa actaccacca
     8161 atagctccaa ttattcctat agggaaagca agaaataata gtataaatcc tgcaattaca
     8221 ctacttttaa ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa
     8281 gaaaatattg agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta
     8341 tttcctggaa agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca
     8401 actaatttta agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga
     8461 ataaatgctg atactgcaac tccccacatt cctgatgtat aagtatatat aatgataact
     8521 ataactccta taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca
     8581 aaagaaatta attgactacc cataatagct agtatagata ttggtgttac aagactttga
     8641 taagctctta aaaatatact atgaccatat ctagtttcaa tataatcagc aggtgttata
     8701 tcacaagata agcttcttaa ttttcctgcg aaaaatgcat ttataaagca agctaaagct
     8761 gcacctatag tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca
     8821 ccaactaagc accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa
     8881 ggcagatttc tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa
     8941 aaaccaatcc atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct
     9001 ttataaaata ttttaataaa atatacaaaa tatatttaaa atatatttta tataaaagta
     9061 gtacgatatt tgttttattg gttttagatt aatatagcta taataattta tgatattaga
     9121 tggaaattac gtaaaaatag aaaattgaaa attctgaaat ttttgcatat tgatatttat
     9181 ctcatagatt cttttttgta attgtaacac gcttttattt ttaaatcaat agtacatgta
     9241 taaaaataaa aggaatttaa ataagaaaaa gatattttta atatctggat gtagtataga
     9301 aatatcttga ataaattatt cttgaataaa attataaaaa attaaaatca ctagaaatat
     9361 gataaaataa tttaaaatac gcatttcaat gacatttcaa aacaagaaaa taataaaaaa
     9421 caaacctaaa ttaagtctaa atagcttttt agttaatttt agtagtaaaa aataaaactt
     9481 aaaaagtgtt tgacaattat gaattttgag aatataatta aattaaaatt atacacatat
     9541 aaaaatatac tcctgaatca agaatgagat atttaagatt caaaatatat attattatac
     9601 gtgaataata aaaagtaaga aaattttata agggaggggt agatgtgagt agtcaagaaa
     9661 gcaaaccaaa aggaaatctt atatcaaatt ttagagatga tatgagagac cctaagaaaa
     9721 gagcaatata cacatattca agttctataa acttagcgta caattttata ggttactttg
     9781 tatcaactgt tacaaatatt tttttaactg atgtggcaaa acttggtgta attatggcag
     9841 gtggtatgca aattatacaa tctttcataa aaattgctgt agctcctata acaggtacag
     9901 tatttgacaa acaaccattc aaaaaaggaa agtattatcc atggcttaga tatgcaccag
     9961 cagcgttagc attaacatat ggaatatttt tcatagttcc attaatgaaa ataagtccag
    10021 cattactttt acctttgatg acagctttat tattaatagc atcttgtgct cagcaagtag
    10081 ttttcactat aatttatgct atttaccctg tagttgctaa gacacctaga gaccgagtta
    10141 taggctcaac tactacgaat atatttaaag aagttggtaa attcttagta ggtttttcat
    10201 atccgttatt attagtttta tttgcagatg tattaggtgg agaaggaatg ggttatttag
    10261 gtacctactt attctttgca gcaggatgcc ttttagtatt ttggttttct tcaacagaga
    10321 taataaaatc tggagcagaa gaagaagttc ttcaaaaaga agagaagcca aagattaaag
    10381 caagtgaaat ggcaaaagct ttattcacta atgtaccact tatgatggca ttttgtttag
    10441 aatttcttat atgtataaga tctataggat taggaccttt ggctccatat tattttaaat
    10501 atgtaataga agatgaacgt ggattagcag tatttttatc aatcatgcct ttggtaagtg
    10561 tagcattcat gtttttcgca ccagtgttta taaaaatctg tagagagcaa aaactagctt
    10621 caataatttc tttttctata tgtgcactat gccacttatt ggttgcatgg actccttggg
    10681 ggaaaactac tttaggagta actatgttgt tggcaattgg tggaggtttt tcaaatgtag
    10741 tatcaataat aaatcttaat ttctttgccg gttcttgtga ttatgggcat tggaaatcag
    10801 gaaaagactt acctggctta agtatgtcac tatatccagt tgcaatacaa gtaggagtat
    10861 tacttgcaac tactataaga acagttttaa tgaactcaat gggatatcaa gctgatatgg
    10921 tagtcacaga agcagttaaa tcaggtttta taaatatgat ttcttattct atggcaatac
    10981 ctttaataat agctgttgtt atagcaatac tatatccagt aagtgacaag aaattaaacc
    11041 aaataagaga agagttaaat caaagaaatg cttagttaaa agaaagttaa gtagatggtt
    11101 aatttaagct attaaagtaa gtagacgcaa tagcgtttat ataaatgaaa atacatatac
    11161 agagggggct tttaaaaatg atagaaaaat tattgagaaa taccgtaaaa gaattacatc
    11221 aatatgttcc aggtgaacca atagaaaaag ttaaggaaaa atatggtgta aaagaaataa
    11281 taaaattagc ttcaaatgaa aatcctctag gaccatcacc aaaagcaata gaagctatga
    11341 ttgaaatgtt aaaacaaggt caactttatc cagagccaga agctaacgaa cttagaagaa
    11401 aattagctga aaagttagat ttaaaaccag aaaattttat tgttgctaat ggtgcagata
    11461 acgttatcac tctaataggt gaggctttta taaatagagg agatgaagtt atatattgta
    11521 acccaacatt tccatcatat agaactgcaa ctataaaaaa tgaagggata ccagtagaag
    11581 tacctttaac agaagattat aagtatgatt tacaaggtat attagataag attacagata
    11641 aaacaaaatt aatatgtgta tgtaacccta ataatccaac aggaacaatt gtggatgata
    11701 aagagttaga agaattttta aagaaagtcc ctgaaaatat tataactata ttggatgaag
    11761 catatataga atttttaaca gttcctaatt atgtggatgg acttaagtat gtaagggaaa
    11821 attataacgt tatagtaaca agaactttct ctaaaatata tggtcttgca gggcttagag
    11881 taggatatgg tattgctaaa gatgagataa taagaacttt atttacagta aaagagcctt
    11941 tctctgcaaa tcgtgttgct ataagtggag caacagcagc acttgatgat gaagagttta
    12001 taaaagaaac atatgaatta aatagagttg gaatggctta ctttaaagaa gagtttacaa
    12061 aaatgggatt tgatgttgta gattcacaat caaatttcct ttatgtagat atgaaaacag
    12121 atatacctaa gttatttgaa gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta
    12181 cacatgcacg tgtaagtatt ggaactatgg aagaaaataa gaaatttgta gcagttttaa
    12241 aggaaatatt aaatattaaa ggagagccaa gtattggata atagatagaa atattatgaa
    12301 acggggtgta gatatgaact taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg
    12361 cgttatatct tgtaaaaaag gagcattaaa aatatcaagt aagataaata aagaaggtta
    12421 tgctcatgta gaagttgatg agagtaaatg catactttgt ggaatttgct atcaggtgtg
    12481 tccagataat gtttttgaaa taattaaaga agaagcatgt caagaggctt agaaggagtt
    12541 gggataatat gaaaaagatg tggaagggga accacgccat tgcagaagca gccctaagag
    12601 gaggttgtga attttatgca ggatatccaa ttacaccaca aacagaggta atggaatttt
    12661 tatcacatag aatgtctgaa cttggaagaa cttttataca gtccgaaaat gagatggctg
    12721 ctatatatat ggtatatgga gcatatgcaa gtggaatgag gtctatgacg agttcttcag
    12781 gaccaggaat atctttaaaa caagaaggga tttcttactt atgtgcaaat cattaccctt
    12841 gtgtaatcgt aaatgtacag agatgggggc ctggtcttgg ctcattggac tcagcacaga
    12901 cagactatct aagagatact agagggggag gaaatggaga ttatcatttg atagtttatg
    12961 caccaaacag tattcaagag actgtagatt tgatgtataa ctcatttgat gtagcggaga
    13021 agtacagagt gcctgttgaa attttgacag aagcagcttt aggtcaaatg atggaaccag
    13081 tagaatttcc agagtttaag aagagagaag gagatttagg ttggacttat gatggaagta
    13141 atagagacca tgcaaaggtt gcagataacc aaaagccaac tttttgtatg gagaaacgta
    13201 tgagaataac tgaaaatgaa caacaatggg aagattatca agtagaagat gcagaatatg
    13261 tgtttgtagc gttcggtata ccatcaagaa caactatgaa tgctgttaga agacttagag
    13321 aacaaggtga aaaagttgga ataataagac caataacagt atggcctttc ccatataaag
    13381 catttgaaaa ggtaagtaaa gatgtaaaag gatttatctc agtagaatca acagacacag
    13441 gtcaattagt agaagatgtt gctttagcct caaagaaagt atgtaaagaa aatgtacctg
    13501 tgtatggttt atttagtgga aatcatatac caaaaacatt gcaagtaatg gacacttata
    13561 gcaaaataaa atctggcgaa atcaaggagg tgttctaata tggctacaga aaaattaaca
    13621 cctagcgttg tttcaatacc aaacaagttt tgcccaggtt gtggtcatgg aatagtaaat
    13681 cgtataatag cagaggtaat agaagaacat ggatatgaaa aaaatcatgt attaacatta
    13741 ggagttggtt gtgtctgcaa tatgaatttt agctggaatg gtgataagat gcaaactgca
    13801 catggtagag cttcatctac agctatagga gttaaagtag cacttccaga tacattagta
    13861 atgacttacc aaggtgatgg agatgcttat gttataggtc tttctgaaac tttaaataca
    13921 gcatatagaa atcataatgt gactgtattt gtaataaaca ataataattt tgcaatgact
    13981 ggtggacaaa tgtcatggac cactatgccg ggtcaagtca caacaacaag tgtaaatgga
    14041 agagatataa agactactgg tagtccactg aaagttcctg agatggtggc aaacttcttt
    14101 gatgtagcgt atgtagcaag aggttcagtt catagcccaa aggaaataat taatctaaaa
    14161 aaatatataa aaaatgcaat agaagctcaa ttaaatggag agggatattc cttagttgaa
    14221 atattagcac cttgcccaac taactggggt gtatcactag agaaatctat caaatggatg
    14281 gaagaagaaa tagtgccata ttatgctctt ggcgaattta agcaaaggga tggtgaatag
    14341 ttatggaaaa agagctagta tttgcagggt ttggtggtca aggagtttta actcttggtc
    14401 tgattgtagc tgagtcagca cttgaattgg ggaaacaagt aacttggatg cctgcatatg
    14461 gtccaactat gagaggtgga aaagcctata gtgtagtaaa gttttctgat gagagtatag
    14521 gtgggcctga tatggaagaa atagatgttt tagtagcaat gaataaacca tctttagatt
    14581 atataaattt agttaaagaa ggtggaacag tagttattaa tacatctgca atagatgaga
    14641 atgtaacatt aagagaagat ataagtgtag ttaaaataaa ttgtcaggaa ttagctcaaa
    14701 aagtaaacaa tccaaaagca gcaaatatag tagtacttgg agcattaatt gctaaaactg
    14761 ggttatttga aaaagaatta gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa
    14821 aaggaaagtt taatgttcaa aatgaagcag cattcatgga aggctataat aacgccattt
    14881 gataaattac ccttatctta agtgatttat ctcaagattt aataatcttt agtgccaaga
    14941 gaaacttcat cttgagataa aattcactta aaatcacaac tataaacata taggagcgtg
    15001 aatttatgag tttggaaaaa cttattagac caaaaagtat tgctatagta ggagtgactg
    15061 ataaacttgg ttttggaaga agtgcagcac ttagtatagt aaaaagtaag gaaacagata
    15121 gagtatatta tgttaatccc aaaagagaag aattgtttgg gagaaaatgt tataagacga
    15181 tacaagaagt tccagaagta gtagactgtg tagtagtatg tacgcctaga aatgtagttc
    15241 catctgtatt aaaagattct ggagagttag gaacaaaggc agtagtggta tatgctagtg
    15301 gatttgcaga ggaaggtaca gaggaaggta cagatttaga aaatcaactt atagaaatat
    15361 caaatacata cgatatgaaa atattgggtc caaactgcat ggggttgcta aattgtatag
    15421 ataaggttaa tctatgggca ggaggttcta aatgggattt agatactaaa aaacctggta
    15481 taggaattgt aggtcaaagt ggatttataa ctgctgaaat tgtttcaagt gattatttta
    15541 atatatctta tggtttttca acaggaaatg gaaatatagt tacactagaa gatgcagtag
    15601 atttccttgt agatgatgat tatgcatcag taatagcaat ttaccttgaa ggtttaaaaa
    15661 atcctcaaaa atttatagat gctttaaaaa gggcagcaca aaaaagaaag ccagtaataa
    15721 tattgaaatc aggaaggtca gaaaaaggtg ctatttctgc tgcttctcat acaggaaatt
    15781 tagctggttc gagtaaagct tttgaaagta tttttgaaaa atatggagta atttctgtgg
    15841 aaaatttaga acagtttatg tgtttagcac aagcttttag tgttttagat ggtaatttac
    15901 ctgcaaattc taattttgca gcaataaact tctcaggagg agaaaatact atttgtgcag
    15961 atttagcaga agaaaatggt gtagaacttg cagaaatatc ttctgaaact aaagaagaaa
    16021 tgaaaaaata tcttccagga tttgcaacac ctaaaaatcc actagatgct acaacagcat
    16081 tattccatga aaaggatatg atagtaggtt tattacatac ctttgaaaag gatgatagtg
    16141 ttggatttac tatggtaggt gcaaatataa gagatgaaga aaatgaaatg catgaaaccc
    16201 tttgtaaagc tgtaagtgaa gctagagaac aaggattgaa aaaacctgtg tttgcagttc
    16261 ctacacttga aggaaataga tatttagatt ataggactag attagaagat aatcaagtgc
    16321 ctataatgtc atcagttgga actacattta cttgttttaa taaaatggct aagtttatag
    16381 attatgatta ttcaaaaaga actttagaat ttaaagctgt taagaaaaga gagtcaaata
    16441 atgtagtagc attatcagaa ttagatagta agattgagat gaaaaaatat ggagtaccag
    16501 ttccaggaca aggtaatgca aaaagtattg atgaattaga tgaaatctta aaatatatta
    16561 agtatcctgt tgttttaaaa ataaattctt ctgaaatatt gcacaaatct gatgttggag
    16621 gagttaaaat aggaattaag aatagagatg aggcagtaga tgcatataat gagattttaa
    16681 ctaatgtaaa aaaagcaaaa ccagatgcta atattgatgg tatattagtt caagaaatgg
    16741 tagaaagtgg tatagaaatt attattggta taactaatga tgaccaattt ggaccaatgt
    16801 tattagtcgg tttaggagga gtatttgtag aggtatttaa agatactaca ttatatccat
    16861 tacctataaa tcatgatgaa gctattatga tgcttaaaaa actaaaatca tttaagcttt
    16921 taaatggcta tagaggaagt gagccttgcg atattgatgc tcttgcagat atgatggtca
    16981 aattaggaaa atatgcttat gaaaataagg atgaagtcaa ggaaatagac ttaaatccag
    17041 tgtttgtata tcctaaagga aaaggcgtat gtgcagtaga tgccttaata gttaaatata
    17101 aatagtttat aagttttggt attcaaccat aattaaagca ccgactgaaa atcacatttt
    17161 gtaagttaga tagtgtgatt ttcagtcacc ccaaatttaa tttaaaaata ttttaaaatg
    17221 aaagtatagt tttaagtttc attgtgaaat agcactgtat aaacaataaa atactacgta
    17281 taaaacgaat aataaaaggt gttttaatga taagaatcaa tcttgatttt cattaaaaca
    17341 ccttctttat tatattaaaa atttttaaac gttatattgt cacaggtggg tttgttggag
    17401 ctgcatatgt acgattagca aattcttggt caacaaagaa tagaccaccc tctccggcaa
    17461 gtttaacacg ttttatattg tcttcacaat ttgtctcttc ttctgcttgt tcagatataa
    17521 accaaagtaa gaattgagaa gttctaaagt ctgattcaga gtttgctaca gatgctaagt
    17581 tgtttattaa agctgtaacc tttttttcat gagctaacgt acgctcaaga atatccattg
    17641 cagaagtaaa gtctgaatct ggcattggga tagcatcaag ttgaaccttt cctcctacat
    17701 tatgtaagta tttgtaaaaa aacatagcgt ggtctgtctc ttctttatat tgaacataaa
    17761 accaattaga aaatccttta agtccttctg cttcaaggta agaagacata gaaagataaa
    17821 gatacgcaga atataattca tggttaatct gctcattaag taatttttcc attttagctg
    17881 aaatcatttt tatacctcca aattttaaat gctcttataa aatataataa tattaaatgg
    17941 aaaaatattc aataaaagtg tttaaatatt ttacatattt aataaaaatt tatactacca
    18001 ttaaaaaaat tataacttac aatggctata gagccaatta atggtgcatt tgctccaaaa
    18061 tttgaatgcc ttacacttat ttttttatat ttagaaaaac ttgctgaagc agtcaatttt
    18121 tttaataatg tattttctat aaatgtacca ggtatattaa agtcatagcc tattattata
    18181 gaactacaat caatcaatgt aagtgtatta actaatgcat atgatacgta tgtacaaaat
    18241 tcatcaagca aaaacatggc aagactatct ttattatttg cctcatctat taacttgact
    18301 aaagaaaaat ctttacagtt aattaatggg gagttaggat aaatatggct caatgattca
    18361 attcttttaa ttagattact gacacttgta taataatcta aacatccatt gttaccacaa
    18421 ctgcacaatg gtccactaaa attaattgat gtatgtccta tttctccact ttgtcctagg
    18481 ttgccagtat gaagtttatt ttctagaact agaccagctc caataccatt cattatgtgt
    18541 aggtatatat aatttgatat atttttacct aaaccataca ttttttctgc taatgcaccc
    18601 gaattagcat catttatcaa aaatattggc aaatcagaca tttcttttat aaaatctact
    18661 atatttaggt ttgatacatt tccaaaatct gggggattta gtatggtacc atttatatca
    18721 tctactggac ctattgaaga aataccaatt ccaattatat ctctcttgtt gattttcatt
    18781 attttagtaa acattctttt tattatatct tttaaaacat catttgatag acatttaggg
    18841 taagtttcag aaagttgttt tacaatatta ccttttaaat ctgaaataat taccttacat
    18901 atatttcttc ttattaggat tccacaaata caaggcgata tattagaaat atctaataaa
    18961 atgggtttac gacctgatga atgtgcattt aaagttgatg actctatttc cctaatcata
    19021 ttttcattaa taagttcagt aactagattt gataaagtca ttttagtaag tcctgtagac
    19081 ttagctaaat caactctgga cataggtgta tttgtagaaa gtaatttcaa tattattaac
    19141 ttatttttaa tttttacatc tttgatgtta gaaccttgtt tgttactcat cataaatctc
    19201 cttaaataaa gttaagtgtt ttatttattt tcaagtatac tatcacctta taatagagta
    19261 acattttttt acttgaaaat atcattaatt agaatatatc acattatata tcataaaata
    19321 tataaaaaaa tgttagaatt aaattaaaag tgaaaagtag attgatatat aaatagtaaa
    19381 tacttaatta aaaaaagata atgatagcaa taattattgt taccaagaat ctactacttc
    19441 aagtgttacc ttaggtattt aagttgatag tggtttgcac gtaataaact tgatgtaata
    19501 tatattctta ataatattga taaaagattt acttaaaaat aatatagatt atgtaaacat
    19561 ataatctatt tattgtatac aataaaattt cattttgtaa aacagtgaat taaatatgta
    19621 atatagatat cgatttttat tacaaattgt tactaaatat aggtaca
//
LOCUS       DAKBNN010000059        19257 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000059 DAKBNN010000000
VERSION     DAKBNN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.059"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            167..796
                     /locus_tag="OUB02_001767"
     CDS             167..796
                     /locus_tag="OUB02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885528.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            797..1579
                     /locus_tag="OUB02_001768"
     CDS             797..1579
                     /locus_tag="OUB02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885529.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1572..2450
                     /locus_tag="OUB02_001769"
     CDS             1572..2450
                     /locus_tag="OUB02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885530.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2483..3442
                     /locus_tag="OUB02_001770"
     CDS             2483..3442
                     /locus_tag="OUB02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8885531.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLIANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3756..4448
                     /locus_tag="OUB02_001771"
     CDS             3756..4448
                     /locus_tag="OUB02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885532.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4458..5462
                     /locus_tag="OUB02_001772"
     CDS             4458..5462
                     /locus_tag="OUB02_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885533.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5710..6561
                     /locus_tag="OUB02_001773"
     CDS             5710..6561
                     /locus_tag="OUB02_001773"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCT8885534.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQV
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6665..7411
                     /locus_tag="OUB02_001774"
     CDS             6665..7411
                     /locus_tag="OUB02_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885535.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     YIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7411..9327
                     /locus_tag="OUB02_001775"
     CDS             7411..9327
                     /locus_tag="OUB02_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885536.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKEDVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9606..10523
                     /locus_tag="OUB02_001776"
     CDS             9606..10523
                     /locus_tag="OUB02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCT8885537.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            10601..11338
                     /gene="cas6"
                     /locus_tag="OUB02_001777"
     CDS             10601..11338
                     /gene="cas6"
                     /locus_tag="OUB02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HCT8885538.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11344..13221
                     /gene="cas8a1"
                     /locus_tag="OUB02_001778"
     CDS             11344..13221
                     /gene="cas8a1"
                     /locus_tag="OUB02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HCT8885539.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLESLVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQEYVSSICDVCKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRVLINSYKKG
                     VYKMDSKNLEKNDEKLKIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            13223..14137
                     /gene="cas7i"
                     /locus_tag="OUB02_001779"
     CDS             13223..14137
                     /gene="cas7i"
                     /locus_tag="OUB02_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HCT8885540.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRADGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14139..14840
                     /gene="cas5b"
                     /locus_tag="OUB02_001780"
     CDS             14139..14840
                     /gene="cas5b"
                     /locus_tag="OUB02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HCT8885541.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKVLN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14851..17172
                     /locus_tag="OUB02_001781"
     CDS             14851..17172
                     /locus_tag="OUB02_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCT8885542.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            17187..17738
                     /gene="cas4"
                     /locus_tag="OUB02_001782"
                     /pseudo
     CDS             17187..17738
                     /gene="cas4"
                     /locus_tag="OUB02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
     gene            17741..18721
                     /gene="cas1b"
                     /locus_tag="OUB02_001783"
     CDS             17741..18721
                     /gene="cas1b"
                     /locus_tag="OUB02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HCT8885543.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18724..18990
                     /gene="cas2"
                     /locus_tag="OUB02_001784"
     CDS             18724..18990
                     /gene="cas2"
                     /locus_tag="OUB02_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HCT8885544.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
BASE COUNT         7890 a         1690 c         3359 g         6318 t
ORIGIN      
        1 ttaattaaaa taaattacat atactattta atatttcatt taaaatatta aaaaatgtag
       61 ataaaaattg ttttattaag aaaaaattaa tatattgaca aaatttaagt gctcatttaa
      121 actattaatt ggtaataata ttcaatatta aataatagga ggcgttatga ataaatatct
      181 tctaataaat ccagtggcag aaagaatgta tggagaaaag tttcacctta ttaaagcaaa
      241 tttgataaaa aaggggtaca taatagcaga atgtgaacca cagttagaat atgtaaaaaa
      301 gcagtacaaa gagtatacaa aaaagactaa aaatacaatg ttggattgta gatgtccaga
      361 aagtataagt cttttaaaaa gaaataattt gataaatgat tttgaagtac ctatgataga
      421 accaatatta gtaaggactt gtagggtttt atacgataaa tatataaaaa gtaaagatga
      481 tatgttggta gtaacctgtc catgtacaca attaagagac tttgcaagtg aaaagtttaa
      541 ggataagggt aatgctatgt tttatacatg gaaagagttt gctgaacaag aaggtgtaaa
      601 atcacttgga aaaatagatg aatcaccaat tccattagga tatttcaaag atacttttga
      661 aaaagttctt gaagtatcaa gtgaagacga aatattaagt gaaatacaaa aaataagaaa
      721 tggctttgaa gataagtatg acatagtaga aatgttttat tgtaaagatg gttgtaataa
      781 tggagatggt ttgtaaatgg tcaataaatt taataataag tttttaaggc tagtaaagag
      841 tgttctgata ttttttataa ttcttttatt atggaaaatt acaaattatc ttggtatttg
      901 gagtgattat atattaccat cacccgaaaa ggtatatagt acatttttaa atatgataag
      961 tgatggttca atatttatta atgtatatgc aagtatgaaa agagttttga taggttttgc
     1021 aataagtact gctataggtg tgcctttagg gatattcttt ggaatttata gtggagtata
     1081 tgaatacttt aaatctctaa taaatttttt aaggaataca cctccccttg ctttaatacc
     1141 aatgcttata ttatggtttg gtataggaga ggaatcaaaa attataatta tagttttagc
     1201 atcctttttc ccaatcttta caagcacatt gaaagggatt aaaaattgtg actcaaaact
     1261 gattgaagtg ggtagagtat ttgagttttc aaagcttcag ataatattta aaataataat
     1321 accaaatgcc attcttgata ttgctgttgg gttaaagtta gctcttggat atagctttag
     1381 agcaattatt ggggcagaac ttgtagcagc ttcatctggt cttggatact tgatatctga
     1441 tggaaaagaa atgtctagaa cagatgttgt tattgtaggt ataatcgtaa ttggactttt
     1501 aggaataatt acagattaca tattttcgat tattgtgaag aaggtaagta agggaaagat
     1561 ggtggaagca tatgaataat ttagtatgtg actctggata tataattaga aatttatata
     1621 aaagttataa agttgacaat gaagaacatg tagttcttag tgatatctct ttaaacatag
     1681 atagaaacca tataacagta attttaggtg aaagtggttg tggaaagact accttgctta
     1741 gagcaattgc atcacttgaa ggtataaata gtggtgaaat taggtttatt aatgataata
     1801 aagagtatag acctaatgtt ggttttgttt ttcaggagag cagattaatg ccttggttaa
     1861 acgtaagtga aaatatagct tttcataatc aaagaaaaaa tactatttta aacaaggtgc
     1921 ttagaaaatt taaacacaat aaaagaatta gcagtaaaat tataaatgat aattttagaa
     1981 gcttagaaac aaatgataat ttgaacaaat caaataaata taataggaaa agtaatattg
     2041 atgtagaaaa atatcttaag atgatgaact tgcagaaatt taaaaactct tatccaaatg
     2101 aattgtcagg aggtatggca cagagagtaa gcatagctag agctctttct ttcaatccag
     2161 atatgttgct tatggacgag cctttttcgg cattagatta ttttacaagg attgatatgc
     2221 aaaatgaagt tataagaata cataagtcaa ctaataaagg cgtgatattt gtaactcatg
     2281 atattgatga agcgttaaag ataggcaaga aaatagttgt tttcacagac gaaagaaaaa
     2341 taaaagaatt taatatagaa gatggttata atagagattt aacttcagat tactatatta
     2401 aattaaaaaa agatatttta atgacaatga aatcaaataa aggcatttaa agaaattaat
     2461 aatattttaa ggagtaaagt aaatgaaatt aaaaaagcta ttgataggta taacgatatt
     2521 aacacttgca acagcatctt tagctggatg tgctaaaaaa aataatggag aaaaactttc
     2581 agaaataaat ttaacttatg taaaatcacc attaaatgtt ccttctataa ttcagaaaca
     2641 ggatgattta tttggaaagg aatttaaaaa ggataatata aaagttaact tccacgaaat
     2701 tactacaggt ccagagcaaa cacaagcttt agctgctgga gaaatagact tcttacatgc
     2761 actaggtggt acatcagctt taatagctgc atctaatgga gtagagttaa agatattaaa
     2821 cacttatagc aggtcaccaa aaggatttat gattttgact aataataatt caataaaatc
     2881 agcagcagat ttagtaggta agaaggtggc aggcccaaaa gggactatat tacatcaggt
     2941 attgatttca gcacttgata aagaaggatt aagtatggat gatgtggaat ttgttaatat
     3001 gggtattcca gaggcatcag cagctctttc tgatggcagc gtagatgctg cattgattgc
     3061 tggtccagca gctcttaaag ctatgaagtc aggtagtaaa cttatagcaa atggtgaagg
     3121 tttagtagat ggtataatag taactgctgt tagcacagac tttgcagaga aacatccaga
     3181 aatagttgaa agatttatga aggttgaaaa ggaaacttta gaatatgtaa ataataattt
     3241 tgatgaagct atggaaaaag tagctaaaga agttgattta agtcttgaag aaactaaaga
     3301 attatatgct tggtatgatt ttagtttaga tataacagat aaagacatat cttcgcttga
     3361 agatacacaa gacttcttaa taaaaaataa gttacaagag aaaaaagtga acataaaaga
     3421 attaatctat aatgctaagt agaaactatg ataaaataat actaagtaag aaatataata
     3481 aaagtttaag ttaccttatt aaggtaactt tttctatttt gaattagtaa tttcaattta
     3541 ttttagaatg attagttgat aataatgatt aattaatcat aaaaagatac ttttttatag
     3601 agttttatta agatgtctaa taatatggtt atacttatga agtcagtaat tgtgaagtta
     3661 gtaatatgat taaatttata tattgtaaaa agattataat atattaaaat tggactgtta
     3721 tataatgagt aaataggtat attaggagga attatatgag ttataaagta tatattgtgg
     3781 aagatgatat atcaataagt acattactta aagattatat tgagaaatat ggatttgaag
     3841 tgattgttga agaaaacttt gatgatataa tgattacatt tgaaagtttt aagccacact
     3901 tagttctatt agatgtaaat ttacctaaat ttgatggttt ctactggtgt agatgtatta
     3961 ggcaacagtc gaatttacct ataattttta tatcagctag agatggtaat gtagaccaaa
     4021 ttagagcttt agaaagtgga gcagatgatt atataacaaa acctttttac tatgatgtag
     4081 taatggcaaa aataaagagt catataagaa gggtatatgg tgaatattca cctaaaatag
     4141 acgaaagaat agtggagcta gatggattaa agttttatcc agaaaggtta gaactagaat
     4201 taaatggtga gaatctaatt ataactaaaa aagaaggtat actgattgaa tgtcttatca
     4261 agagataccc aaaagtagta agtagagatt atcttttaga aaagatatgg gatgatattg
     4321 agtttgtaga ggaaaatact ttaaatgtaa atgttagcag gataaggaaa agatttgagg
     4381 aattaggtat agataatgct gttcagactg tccgagggtt aggatataaa ttaaataaga
     4441 attggtaagg agaaagtatg aagttatttt taaaagatta taaaggttat atttttatat
     4501 attatatagg catagttatg acaattattt actgtaatct tatgaagttt attagttttg
     4561 gagagagttt atatattctt ttatttagtt cttttatatt gttatgtttt ttaatatata
     4621 agtattataa aaatagagaa gtatataatc tgtttgaaaa aggagtacat tctttggagg
     4681 agtcattttt gtatttagga agctcattct tggggagaaa tatatcagtt attttaaaac
     4741 aacaacataa attgtatcaa tcaatgatac aagagcataa aaagatacat agtgaacatt
     4801 taactttcat caaccaatgg attcatcaga tgaaaacacc aatatcagtt ataagccttc
     4861 agcttcaaga ttatgaggga gaagaagtaa ctgaaaatat aagaaaagaa atagatagat
     4921 taaataaagg gttagatatg gctatgcatt ttgcaagggc agataatttt cagaaagact
     4981 ttatagttga aaaagccaaa ttaaatgaaa ttgtatctag tattgtaaat gaagaaaaaa
     5041 ggttatttat aaaaagtgga atgattccta agattcaagt agacaaattt acttatgttt
     5101 atacagatgt aaaatggatg aaatttataa taggtcaatt aattacaaat ggagtgaaat
     5161 attctaaaga ttatagtaac tacctaacta taaaatctga agaaaataat aaatatgtaa
     5221 ttttaagtat aatagatgaa ggaattggaa ttgtttctaa agatttaaaa agggtatttg
     5281 acccattttt tacaggagaa aatggtcgta agtttggaga atctacaggt atgggtcttt
     5341 atctagtaaa aaaggtatgt gatgatttag gtcataaagt aagtataaaa tcagaagtag
     5401 gaaaaggtac agaagtttca atctacttta ataaagatgt aaatagttta ataaaggtgt
     5461 aaattatctg ttacaatagg gtttttagtt aattgtactt ttttattata caatttatta
     5521 aatgaaatat tcatagttaa ataatcaaaa tttaagagag tttatttgca aaccttacat
     5581 agatgtaagg tttgtgtaat gtttgcagat agttaaattt tctaatctaa attacaatta
     5641 atataagaaa taacataaga gaaataacat aaaaaaagaa attttgtaaa aaaggaagga
     5701 tgtttattga tgagtttaga tgttattttt atattgaaat cagtcataat agctatagta
     5761 gaggggctta cagaatttat tccagtttca tctacaggtc atatgatttt ggttggtaat
     5821 ttaatagatt ttaaggggca atttgctgaa atgtttgagg ttgtaataca acttggggct
     5881 atattagcag tagtagtact ttattggaaa aagataaaag atagcgttat agaatttttt
     5941 aaatttatat ttactggcgg aaaagaagga aagattggtt ttagatttgg aatgaatgta
     6001 attataggtt gtataccatt tgcgattata ggagttctat tttacgataa tataaaatca
     6061 ttgtttaatt tacaatctgt aattatagga tttatagtgg gtgggatttt attattagta
     6121 gtagaaactt tgtttagaaa aaagaatcat tccacagata atatagataa aattactcca
     6181 atacaagttt taaaggttgg gacattacaa gttttgtctg catggccagg tatgtcaagg
     6241 agtgcatcta ctattatggg tggatggata gcaggactta attctcctac agcagctgag
     6301 ttttctttct ttttagcagt accagctatg gtggcttctt caggaaaaga tttatttgaa
     6361 tttgattatt caataatgac accaacattg tggattgctt tggtagttgg gtttatagta
     6421 gcttttattg tatcaataat agttatggaa aaattcgtga attttttaaa gaaaaaacca
     6481 atgagagtgt ttgctgtata cagaataatc atgggtgtag tacttgcagt attagctttt
     6541 actaacataa ttagtgtata gaaaaaatag atttattatc tataaaaatt aaactaagat
     6601 aaattataat taacaattat atatatgtta tttcaaaaat taaattagga aggatagtta
     6661 aataatgaaa attttaactg taaataattt aagtaaagta tatggtaaaa aaataatttt
     6721 caacgcatta aatgacataa attttagtat agaagatgga gaatttgtag gaattatggg
     6781 tccatctggt agtggaaaaa ctactctttt aaatatgatt tctactattg acaagccaac
     6841 tacaggaact atggaattaa agggcaagaa tcctttattg ttaagaggtg aggaacttgc
     6901 attatttaga agaagagagt taggttttgt atttcaggat tttaatttat tggatacatt
     6961 aactatagga gaaaatatag tattgccatt gacattagat aaggtcagtg taaaagagca
     7021 agatgagaga ttgaatgaag tatctacaat attaggtata aaagatttat taggaaaaag
     7081 aacttttgaa gtatcaggtg gtcaagctca gaggactgct attgctagag cattgattaa
     7141 caatccatca atacttttag cagatgaacc aacaggtaat ttagattcaa agtcatcaaa
     7201 ggttgtaatg gagctatttc aaaagataaa taaagaaaat aaagttacta ctatgatggt
     7261 aactcatgac ccattagcag cgagctactg tagtagaatc ttattcataa aagatgggta
     7321 tatatataat gaaatatata agggtagtag tagagagcag ttttatcaag agataatgga
     7381 tgtacttaca ttattgggag gcgataacta atgaatataa aacagtttgc tattaacaat
     7441 atttcaagaa atattaaagc gtatttagga tacttagcaa gtattgttat atcatcttca
     7501 ctgctatttt cttttattat gtttacaagt catccagatt tagatgtatc tcaatttcct
     7561 gattatttaa aagaaggtct caagatgagt aagataattg catatctatt cttattcttc
     7621 tttgtgtttt attcagttag tgtgtttctt aaaagtagat ataaagaatt tggaatactt
     7681 tatattacag gtatatcaaa gcgccaagta atgaaattaa tctttataga aaatatgata
     7741 ataaatatag tatcttctgt aattggaata ataataggct taatctttgc aaagatattt
     7801 ttagtggtta tgtctacttt tttagaatta tcaccattaa aattctatat accatttaat
     7861 gcgatattgt ctactttaat ctattttatg attttagctg tattaacatc aatatttact
     7921 tcttttatag ttaaagaaaa tcaggtgttg aagttactta aaggtacaaa aactccaaaa
     7981 ccagagccta aaacatcact aatgcttgct atattaagta tttgtttgtt tataatagcg
     8041 tattataatg ctattacctc aaccgaccaa tatacaacat atctgcgctt agttccagtt
     8101 acatccctta ctatagtagc aacttacttg ttcttttctc aatttagtgt attttttatg
     8161 aagaagctaa aattaaacaa gagattttat agaaaaaata caaatatgct ttggatttcg
     8221 aacttgattt ataaggtaaa ggataatgct agaatatttt tcttgataac tataacttct
     8281 gcagtagcat ttactgctat aggtacagtt tattcctttt ggaaagatgt agagcgtcaa
     8341 attaatttaa tatatcctaa cacaatatat tattcaacaa tgacattgca taatgaccct
     8401 aaaaagcctg atagcaaaga aaaagacaag gaaagaatga tgtttataga aaataatcta
     8461 aaaaaagagg atgtagacta tacaaggact aatggtgaat ttaaaactgt atttcccaag
     8521 aaaagtgatt ttaatgtcag aattatgaaa gaatctaagt acttagaaat tacagaaact
     8581 ataggggtta agcctataag ttttgaagat aatgaatgta tatcattatt atcatcaagt
     8641 ttaccaggaa ataataacgt caaagaaaat attattgtag ggaatagagg tttaaaggtg
     8701 gcaaaacaag tggaagaatg tgttatgcca gcatattaca aaaatatgta tgtggtaaag
     8761 gataattttt atgatagtat agaatcaaaa tttatagtgg atagatttac cgcatttgaa
     8821 gtagaggatt caagtaaaac tatagagata tgcagagaat ttgaaaataa atttaataat
     8881 gagtcaggga tgcaaccata tttgtttttc tctaaagaat taatgatagg aactgggaaa
     8941 ttaatgtatt ctacatttat atttttagca atatttatag gtttaatttt ctttgttact
     9001 acaagtagtt ttttatataa taaattttat atggattgcc aagttgataa gaaaaagtat
     9061 gaacagttaa ataaattggg tatgacatat aatgagatta aaaaagcttc aacaattgag
     9121 attggaatag tatttttgtt tccttatgta gtggctgtta tacattcaat atttgcactt
     9181 actgcactga aaaattcttt tgatatagaa gtagcatcat cagctgtttt ggtaatggga
     9241 agcttgttct tggttcaaat agtttatttt ttaattgtaa gaaataatta tttaaaagag
     9301 ataaggttaa atttagtcaa tttttagtta ctattattaa atgataataa aaagggttta
     9361 aaattaatta atattactgg aattatagat gtttattttt tcagtaatat tttttattgt
     9421 attaactatt aaaattttgt acaaatataa aatataatat ggtaaatgtc aaaattataa
     9481 aaaaattcta cttatataaa atataaagag gttatatgta catatgtgta gaaataaata
     9541 ataaaaatat caattaatgt tattaggggg ggttactcat tagtaaatta gctaatacac
     9601 ttagaatgat gatattaatt caaagcagag gcaaaatgaa atgcaaagaa ttatcagaag
     9661 agttggaagt aagtgaaagg caaataaaaa gttacaagat gtatttagag caagcaggta
     9721 tatttataaa ttcaacacct gggatttatg gtggttatga aatagataaa tgtaatagca
     9781 tatcattaat taaattatta gatagtgaag tttcaattct agatatgata aattcacagt
     9841 tagagtataa taatgatatt tacaaaaatg aatttaataa tattgtagaa aaaataaaag
     9901 cagtattaaa tacaggagaa aaaagcgata catatatgga ttattttaca gttcaagcac
     9961 aacgtaattg tgattatgag tctgaaaaaa ataagtgtaa tgaaataatt agagcatata
    10021 ccacaaaaca caaattttgg atagaatatt attcattaaa ttcaggaaat agtgaaagaa
    10081 ttgttcatcc atatggttta tttaattata aatcagatac ttatatggta gctttttgtg
    10141 aaaaaagatt taagtttata gactttaaat tatgtaggat taaagattat aaggttttag
    10201 aagaaaaata taatgtagat aaaagtttta gttgggatga atatagtaaa aatagtatag
    10261 gaatttataa gggagaagaa attaatgttg ttataaaaat tagtcatcca ttttcaacaa
    10321 ttataaagga gaaagtttgg gttaataatc aacaaatcat agaatatgat gataaatcta
    10381 taatgtttaa agctaaaatg agaggttatg aggagataaa aagttggatt ttaagtatgg
    10441 gagcttatgt tgaggttgta gaaccagata gacttagaaa tgatatattg tcagagattg
    10501 aaaaaatgaa aaaaatttac tgattttttc aaataattta tacttggacc aatatacttc
    10561 acggtctagt gttaatatat taattgagag aggtgatatt atgaaaatga aaattgaatt
    10621 tagtacaggt tgtataccaa tatcttataa ttcgctcttt atgagtatta taaaagaagc
    10681 aataagaaaa tctaatgaag attactataa aaacatgtac tattataaag aaaagaataa
    10741 taaaaaaact aaaaatttta cattttcggt ttatataaaa aagtatagta ttgaaggtga
    10801 caattttatt atagaagata aagtaatatt aaatataagt actccagatt tagaattggg
    10861 ttttcatata tacaatgggt taatgacttc taaaaaatgt ttatataaag cctatgaact
    10921 tacaagaatt agaatcgatt tatcaagaga aaagaaggta acagaagaaa gggtactatt
    10981 taatgcattg tctccaatct gtgtaaagtc taaagaagga aaattcttgg agataactga
    11041 tgatagatac atagaagaac taaactatat aactaatgaa gtggttaaaa attatagggg
    11101 gaatggatta aaaagaaagt tagaatttga aaatataggt ctcaaaaagg tagtagttaa
    11161 agagagttta agagaattta aaaaaataac aggtaaagag tatcaatata ttaatggtta
    11221 caaaggtaaa tttatattga aaggtgatat tgatgattta aatcttatct acaatctagg
    11281 aattggtttt agacgtgctc agggatttgg agatgtagat attttagaat ggaggtgaga
    11341 taaatgaagt taagagttta tagaggagat tggtttttta atatgggtat agttggattt
    11401 ttaaatataa taaagaaagc agataaacaa gctgaaatat ttattatgga agattatatc
    11461 gaatttgata gcttatttct tgaaaacttt catgagtatt attttaatta ctttatggat
    11521 gaatatgatg tatcaaaaag aattaaaaaa aatattgatt atagtataaa ttttattaaa
    11581 tctaagcctg atagaatcaa agatggaatt aagaaaatta aagatagtgt gaaacaacaa
    11641 aatgataaga ttaaaaaatt tgatgaagaa aaatttaacc tgataaaaga gaagctagat
    11701 agtatgtcta agataaaaag ttatgatgaa tttgatagtt tagaatcttt agtagatgag
    11761 actatagata tatttaaaat aaaatcaata aatgacaggc ttacagcaaa tctttataaa
    11821 tatgtagttc aagataatta ttttggacaa gttagttttt ttaatgttgc taaggcgaaa
    11881 ttagatttag atggattaaa acaggtaatg tttaatgatt atttaagaca aattatatat
    11941 tttggagagt tagaagattt attagaagag aatgactatg ataagttgaa aaactattta
    12001 aatgatagat taaatagtat agctaaggat ataaatgaaa aaaaagttag taagtctagt
    12061 ataaatacta tagaaaaaat tatgaaggaa ataaataaaa actttataaa gaaaaataaa
    12121 agcatagaag atataaaaga gtatatggat agtttagaag tatgtgaaat gtgtggttta
    12181 tataagggaa tattagatga atactcagaa agtaattttg ctccacttgg agtaagcacc
    12241 aataatgcta ggaatatgtt ttggaatcag gagtatgtat catcaatatg tgatgtttgt
    12301 aagctaatat tgttctgtac tccagcaggg gctacttata caagaaagaa ttatttaatt
    12361 aatgaagaaa atgagtttta tttatttgtt aacatggata cttctataaa tgagcttttt
    12421 gaaagaaata atagtttaaa agctcaaaaa agtgagtctt cagattccaa agatgaaaat
    12481 ccttttaatc agcttataaa aagtatagta gaagaaaata cattaaaaag tgaatggcaa
    12541 cttcgtaata tactttttgt ggaatttaaa gcaagtatag attctaaaaa atgtaaaata
    12601 aattatttta atattccaac ttatttagca aaatttttta cagacaagta tgcaaataaa
    12661 aaaatacaaa gtatctatga ttacaagctt aaatcaaatg tactagattt actcttgaaa
    12721 aatagggact tgaagcatct aactaataac attttaagaa ataaagttaa aaatgatatg
    12781 gaaagcaaca acaaatcaaa tatatcagga atagattgtt ttagagtagt ccaattaaga
    12841 gtattaataa atagctacaa aaagggggta tataaaatgg atagtaaaaa tttggagaag
    12901 aatgatgaaa agctgaaaat aatttattat ttaggttgtg atatacatga ctattttgtt
    12961 aataaaaatt ctaaaaataa aatagatgga gtaagctata aacttttaaa ttcagttaaa
    13021 gtcggaaata aaagtgattt tatggataca attataagag tttttatgtc agctgaaaaa
    13081 cagattccag catttatttt agatatagaa attgaaaaag atttagactt tgaatctata
    13141 ggtcatgctt ttatatctgg attaatttct ggaaaatatg aaggaaaaga taaactacca
    13201 aacgaaaagg aggaaaaata agatgaaaag aggattaaca tttacagtaa tagcacaggc
    13261 tcaaagttta aactatggtg aagggatagg caatatatca gaattaaaaa aattaagtag
    13321 agctgatgga aatatgtata catttgcatc tcgccagtgt ttaagatatg acatagtacg
    13381 attagcagca gaattgttta attggaattt acaggttgtc gataaagaaa aaggtactat
    13441 tcaatttaaa gataatataa gtataagaga atcagaagaa atggatttat ttggatatat
    13501 gaaaacaaat aaaaaagatg agaaagataa aaaaggaggt tctttgacta gagaagctac
    13561 tgtaagatta agtaatgcga tttctcttga gccatataga agtgatatgg attttttaaa
    13621 taataaagga tttgcagata gaataggaga acaccctaat cttgccaaca tagagcaaca
    13681 tttaagttac tatacatata cggttactat agatttatct aaaataggta aggatggaga
    13741 tatagaactt gataataaag agaaatgtag aagagtagta gaatttttag aaataataaa
    13801 agtattgaat agaaatatta gaggtagaca agaaaactta tctcctctat ttgtagtagg
    13861 aggagtatat gaaatagcaa atccattttt cttgggtaga attaaattaa aaggagataa
    13921 aaatggattt aaaataaata agcaagctat agaagaagta attcaaggta cttttttagg
    13981 aaaagattta aaagaattta cctatgttgg aatggtagat ggggtattta taaataaaga
    14041 agaatttaag ggattgtttg aagataattt tttaagtgta gataaatttt ttggacaact
    14101 agttaaagaa gtaaaggaat attatggggt gaattaatat gaaggttttg aaattagatt
    14161 tatttcaaga aactgcatgc tataaaaaac catttgctat gaagattaca gagacatatc
    14221 cactgcctcc atattctaca ataaatggat taattcacaa agtattaaat gctacagaat
    14281 atataccgtt taatataagt gtacagggaa catatgaaag tatatttaat aattaccaga
    14341 caacgtattt ttataaaaaa gatagtatta catctatgcc aatgaatagt catatgctat
    14401 tgaatgtaaa tttgataatt catataggtg gggattttga tttattggaa gaaatatatg
    14461 atagtttatt aaattatgac gagcatttat cattaggtag aaaagaagat ttagtaagaa
    14521 ttaatgatat tagatttgta gaagtcaata aatttgaagt agatgaggat atacaattag
    14581 atgattatga aaattataag ttatcagaat gtaatataag aatgcctata tatataccaa
    14641 aaacaagttt acaagataca gatatagatg gtataagtta taggctcaat aattactatg
    14701 ataataatgc tgtggaagat aaaaaaaggg tttggaataa ggtagatagt tattatgttg
    14761 aggatggaaa tactatatca attggagata tattattaga taatgaagga gatttattat
    14821 attttaataa tataaattag gagggagagt atgttatatg ctaaatctaa tccagtggaa
    14881 acacttagag agcatactga tgaactttta aaacaaatga atgtattaag agaaagttat
    14941 ggaaaaaata taaatagctt agattttcta gaagaggaga tattttggca attacttgac
    15001 tttgttatag aatttcatga tattggaaag gcattttcac cgtttcaaga gttaataaaa
    15061 agcagaatgg atactaaaat aaatgaacct aaaatagtaa ctcatttaga aaataatgta
    15121 ggccataatt atctttctcc tgcatttatt gattatagtt atatagacag gaaaaaaaat
    15181 aaagaactta gagcagtatt aaatcaagtt attgtatatc atcatgaaag agatatcttt
    15241 atagataaag attttaaaaa tttaatacaa aaaatattag atgaagattt aataaataaa
    15301 gtttatgaac tacagcatga atttaaagtt agatatccaa taaaaacaga aagattgagt
    15361 aaagtatatc tacaaagtgt agaaaaaaga atagataaaa atcataagta ctataattta
    15421 tatattatgt taaaaggaat actacataga ctagaccata gtgcttctgc tcatgaagtt
    15481 gtggaatgca ataatgttat aaatatagga gaacagacag aaaattattt aatgaaagag
    15541 tttggtagtt tgagagaggc tcaatcattt gctaaatcta atagaaataa gaatataata
    15601 ctgattgcat ctacaggaat gggaaaaaca gaaactgcct taatttggat agataaagac
    15661 aaggcatttt ttacacttcc attaagagta agtataaatg cattatttga tagagcaaaa
    15721 aatatagttg gagttggaga atcaaatgat acattcttag gtttgttaca ttcaacagca
    15781 atagattatc tagaagaaag taatcaagaa aattcaagtg agattgtaga tttagcaaag
    15841 ttattatctt gtaaactgac attttccaca atagaccaaa tatttaaatt tccttttttg
    15901 tatagaggct atgaaaaagt gtactcaaca ttagcatatt caaaagtagt aatagatgag
    15961 atacaagcat attcaccaga aatagcagct gtattggtaa aaggtataga aatgatacat
    16021 aagataggtg gcagatttat gataatgaca gctactatgc caaccatata tatagatgaa
    16081 ttaaaaaaaa gaggtgtaat gaatagtaac ttagctgact tgacatgtaa cacagaaaaa
    16141 ataagacact gtgtgtctat tgtagaaaat tcaatagatg aaaacttagg taaaataata
    16201 caaagtggaa tgaataaaaa agttcttgta atagtaaata cagttaaaag tgctgtagaa
    16261 aaatatgagt taatagaaga aatagttaaa ccaaaaggaa tagatgtcaa cttaaatctt
    16321 ttacattcta tgtatataca agaagataga gcaaaattag aaaaatatat aaaagaattt
    16381 gcagatagtg atagtaatgg tatttggatt actacacaat tagtggaagc atcccttgat
    16441 attgattttg atgagcttca tacagaaaat tcaacacttg acagtctatt tcaacgattt
    16501 ggtagatgtt atagaagtcg tgagtatgaa gaaaatagcc caaatatatt tatatataca
    16561 gaaaaagcta caggaatagg tagtatttat gataaggata ttgtagaaaa aggacttgag
    16621 ctactgaaaa cttttataaa tggaaaagaa tgtgtgaaga tgaaagaaaa atataaggta
    16681 gaaatggtta agatactata ctctaaagaa agtctagaag gtacagcttt tgaaaagaga
    16741 tttaccagtg ctttaaatat tttagatact ataacgccat atagtttagg ttctaaagat
    16801 gcacaaaata tacttagaaa tattgaagga tatacagtaa ttccagagga tatatataat
    16861 aaaattgaag aaactttaat caaagattat gaagagttgg gacaagagct tataaatgca
    16921 tatagtaatg atgatagaca attgataaat aaaataaagt ctaagagaaa aaaagcaaga
    16981 agagagataa ttaaaaaaac tgtaaattta ccaatatata aagctaaaca aaatgtaatt
    17041 gacatacagg tgaaaggtct tgaagattta aaaatacttt tatataaata tgatatacac
    17101 gaaaataagc acacaaaaca gatatttggt aaaggtgtgc ttataagtaa tgaaatagac
    17161 caatttattt gatttttata ttaacaatgt ggctaaaatt tactaaagaa ggtgattttg
    17221 tgccaataga tttagaagta ttaaaagttc aaggtgtaaa aataaattat tattatgtat
    17281 gtaaaagaaa actatggcta ttttctaaag gaataaccat ggaacaaaat agtagtagag
    17341 tattgtctgg gaagatagta catgaagatt catataaaag aatgaaaaat aaagaaatat
    17401 tgattgatga tatcttgaag ctagatataa taaaggatga gtatgtaaaa gaagtaaaaa
    17461 taagttcaaa gatgaaagaa gcagatgaaa tgcagttata ctattactta tactatttaa
    17521 agaatagtat gggtatagag aaaaaaggaa ctataaatta tgttaaggaa aagaaacaag
    17581 aagaattgat attgacagaa gaaaaagaaa gaattattga aaacacactt ataaatataa
    17641 atgaaataaa tatgtagtta tctcctccca aaattgagaa aaaacattat tgtacaaaat
    17701 gtgcttatta tgaattttgt tttgtgaagg aggagtaaca atgtataaag actattatat
    17761 tataagtaat ggggatataa aaaggaaaga aaatacgatt tactattaca atttagaagg
    17821 tgagaaaaaa atattaccag tagaacaagt tgatgatatt catatatatg gagaagtaga
    17881 tttaaacaca aaacttataa attacataag taaatatggg gtgatgcttc atttctacaa
    17941 ttactatgga tattattcag gtacatatta tccaaggaag aaaaatgtat caggattcag
    18001 tttagtatgt caatcagcct gctatttaga taaaaatgaa agattatact tagcaaaatc
    18061 ctttgtggaa agtgcatcat accatatttt aagaaatatg agatattaca aagttgatga
    18121 aagtttaatt gatactataa aggatgaact aaatgatttg aatgatgtca aagaaatatc
    18181 agaacttatg ggtaaagaag gcagaataag aaaaacatac tataaatcat ttaatcaaat
    18241 attaaaagat gattttgaat tttataaaag agaaaaaaga cctcccaaag acccagtaaa
    18301 tgctcttatg tcttttggaa atagcataat gtatacgaat gttttaagtg agatatataa
    18361 gacacaactt gaccctacaa taagcttttt acatgaacca tcaacaaaga gattttcttt
    18421 aagtttagat attgcagaaa tttttaagcc aattatagtt gacacaataa tttttagcct
    18481 gattaataat aaaagaatta ataagaatga ttttgaatat caagacaaaa tttgttattt
    18541 aagtgaaaaa ggaaagaaga aatttttgca agaatttgaa aataagatgc agacaactat
    18601 aaaacataga aatctaaata gaaaagtttc atatagaatg tttataaggt tagagtgtta
    18661 taaattaatt aaacatttta taaatgatga aaggtacaaa gttttaaagg tgtggtggta
    18721 agtatgtttg ttattgttac ttatgatatt gttgaagcaa ggtcgttaaa tagaattaga
    18781 aggatactta gaaaatattt gacttggacg caaaattctg tttttgaagg caatattact
    18841 gaaggaaagt tacataaatg tatttctgaa atagaaaata ttattgataa tagcgaggat
    18901 tcaatctatg tttatgagat aaaaaatcct aattcaatta aaaagaaatg ttatgggatt
    18961 gataagtatt ctgatgaaat gtttatatag gtttgcagtg agcgatattt atgctaaaat
    19021 aggtgttaac agttggaata taagggattg aaggtgtatg ataactgtta tcaattgcac
    19081 tactgctcgc tcactgcaaa ttttgatgtt tttattgaat tataattgct tgattgaagt
    19141 attttcaatg tattcaatta tacctatttt ggggttttat attaactaag tggtatgtaa
    19201 attttccctg cattaacaaa atcttcaaca ttcaaaaagt tttatattaa ctaagtg
//
LOCUS       DAKBNN010000060        19197 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000060 DAKBNN010000000
VERSION     DAKBNN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.060"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            118..456
                     /locus_tag="OUB02_001785"
     CDS             118..456
                     /locus_tag="OUB02_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HCT8885545.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(583..1872)
                     /locus_tag="OUB02_001786"
     CDS             complement(583..1872)
                     /locus_tag="OUB02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCT8885546.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1952..2656
                     /locus_tag="OUB02_001787"
     CDS             1952..2656
                     /locus_tag="OUB02_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HCT8885547.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2748..3794
                     /gene="selD"
                     /locus_tag="OUB02_001788"
     CDS             2748..3794
                     /gene="selD"
                     /locus_tag="OUB02_001788"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2796..2798,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCT8885548.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3812..5224
                     /locus_tag="OUB02_001789"
     CDS             3812..5224
                     /locus_tag="OUB02_001789"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCT8885549.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5338..7221
                     /gene="selB"
                     /locus_tag="OUB02_001790"
     CDS             5338..7221
                     /gene="selB"
                     /locus_tag="OUB02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCT8885550.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7326..10064
                     /locus_tag="OUB02_001791"
     CDS             7326..10064
                     /locus_tag="OUB02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HCT8885551.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10183..11127)
                     /gene="manA"
                     /locus_tag="OUB02_001792"
     CDS             complement(10183..11127)
                     /gene="manA"
                     /locus_tag="OUB02_001792"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCT8885552.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11300..12661
                     /locus_tag="OUB02_001793"
     CDS             11300..12661
                     /locus_tag="OUB02_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8885553.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12749..14638
                     /locus_tag="OUB02_001794"
     CDS             12749..14638
                     /locus_tag="OUB02_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8885554.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14653..15108
                     /locus_tag="OUB02_001795"
     CDS             14653..15108
                     /locus_tag="OUB02_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8885555.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNSINEALGL"
     gene            15167..15484
                     /locus_tag="OUB02_001796"
     CDS             15167..15484
                     /locus_tag="OUB02_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCT8885556.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15509..16771
                     /locus_tag="OUB02_001797"
     CDS             15509..16771
                     /locus_tag="OUB02_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCT8885557.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16876..17940
                     /locus_tag="OUB02_001798"
     CDS             16876..17940
                     /locus_tag="OUB02_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT8885558.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKALGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17994..19172
                     /locus_tag="OUB02_001799"
     CDS             17994..19172
                     /locus_tag="OUB02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCT8885559.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7726 a         1995 c         3353 g         6123 t
ORIGIN      
        1 attattatta aaatgataat taatatcaat aagtattgac aaaattattt ctaaatacta
       61 ttatatataa tataaataat aatgaaaatc aatatcaaat aaatgggagg aaatctaatg
      121 aacttattag tgattggtgg tcatgaaaga atggaaaaag attatatggt tatggccaag
      181 aaaaaaggtt ataagacaaa ggtatatact accatgtcat ctaaattaaa taattctata
      241 ggaagaccag atgcagttgt catacttaca tcaactgttt cacataagat gtctagaacg
      301 gttgagtctc aagctaaaaa acaagatata ttaatcgtaa ggcataaaaa tagtagtaag
      361 gtagcattta atgaatgtct agatatggtt gatgaatgtc tgggaaattg ttcaaaatgt
      421 aagcttaaag acaaacaaaa acaaaaaaat atataggtat aaaaaataca taaacataaa
      481 attaaaagta ctgagataat agttataatt attatctcag tacttttaga tttataagga
      541 taagtatatt ataaattttt tattataatt tctaactact tgttactttt tcatattttt
      601 tttacttcta gctttttttc tttttttctt ctttatattt gaagctatta taaataaaac
      661 taaaagcact agaatacctg caataatctt aacaaaagtc attaaaacat tattttcagt
      721 caaaaatgga agactgctat caagattatt ttttgcaatt aagtctatac tgttttctag
      781 tttaccatta ttatatacat ttaaagtacc aaccttagct ccttttttta taggcaaatc
      841 aattttattt aactctgttt tggtatcata attgccttga gattcatctt tttctaagaa
      901 tatcctataa ctattttttg gttcataaat caattcatgt tgttttgtaa acaatacttt
      961 tttagtatct gtgtattttt ctttatcaac aatagtagca cacttaaagt tatcaaatcc
     1021 ataatcaatc agaatacgtg agtctaaata taaatcagca ttagttgaat taaatacagc
     1081 aacgataact cttctgccat cttttacagc actcgataaa agacatttac cagcagcatc
     1141 agtatatcca gtttttatac catcaactat atcatatttt atatttatag gttgaccttt
     1201 gtatgtaatc ttatcatgtg atgtcaaaaa cttattagtg tttacaaagt atctctcata
     1261 aggatatgct tttgtagctt caaatttaag tgacttagtt tttacaattt gtctaaaagt
     1321 atcattattc atagcttctc tagctatgag agccatatca tgagctgttg tgtagtgatt
     1381 tgggtctggt aatccatgag gattattaaa atgagtatta gtagctccaa ttttttttgc
     1441 ctcagagttc attaggtcaa taaatttttg tacattgcca ccaccaacat atctagctaa
     1501 tacaaaagca gcatcattag cagaatgaac caaaagagca tctagtaact gctttacagt
     1561 aaaagattcc ccatttttta gatacatact agagccatct atttgaggta aatctttaat
     1621 ttctattact tgatttaaat ctttaacctc ctttagaact aaacaagctg tccatacttt
     1681 agttgttgaa gctggataaa gcttagaatt tccatcctta ttatatagta ttttgccaga
     1741 ctcatagtcc attaaaactg catgtttagc aactatagct ggttcattat ctgcaaaaga
     1801 gatattagag cttcctaaaa atgaagtaaa tatcaataaa cagattaata aaagtgataa
     1861 atttcttttc attgtgagcc tccaaatttt aaattcatct tttagtatag catatatttg
     1921 tcaataatta aaagaaagga gtgattaata tatgaaaaaa aaggtaaaat ttatttcgtt
     1981 ttttgtttta tttattttaa taatatcatc aataatcgtt tataaatcgc ttattttcaa
     2041 agatgatatt tatgttgtaa gtaaaaaaaa tttacctaat gaaaaaacac atgttgtaga
     2101 gaatgaaatt aaaggtgaag ataataatca caataaagta actaaagaca tagataaaaa
     2161 ttttcaaaat aataaaataa ctatatacat aagtggagca gtaaatagac ctggaattgt
     2221 aactatagag tcagacaaga gactttatga tgctgtagaa ttactaggag gaacgacaaa
     2281 agaagcagat ttaaatggag tcaatctatc agtaagatta gaagatgaac aacattacat
     2341 aatacctaaa ataggtgaag caacatctgt tacaagtaat gatgattcca ataaaccaaa
     2401 tcagaagaat gagtcaagag gtgagcctaa aaataaatcc aataaaaata tatctaacga
     2461 atcaaaagta aatataaatg tagcgactat agaagagttg gattctctac ctggggttgg
     2521 agaagctaca gccaataaaa ttttacaaca cagagaagaa aatggacagt ttagttcaat
     2581 tgaggaaata aaaaatgtaa atggtattgg agataaaaag tatgaaaata taaaagattt
     2641 aatttgtgta gactaaaata taatacaaaa cttacatata gatatgttat tataaatatg
     2701 tatgtatatg tgtgtacatg tggaaattta gtaaaggagg cacttttatg aaaaaagaac
     2761 aaaaaaaact tacagagatg acaacagcag gtggttgagc cgctaaaata gggccagagg
     2821 ttctggcatc agttttatct caattgccta aaaatgataa tatagaaaat ttattagtag
     2881 gactagatac agcagatgat gctgcagttt ataaattaaa tgatgatatg gcacttatcc
     2941 aaactttaga cttctttaca ccaatggtag atgacccata cgtatttggt caaattgctg
     3001 catcaaattc actatcagat gtatatgcta tgggaggcaa acctttggtt gctatgaata
     3061 ttgtttgttt tccatcatgc catgatatgg atgtacttgc agaaatatta aaaggtgggt
     3121 ttgataaagt gaaagaaagt ggagcacttt tggttggagg gcatactgta gatgacaaag
     3181 agcctaaata tggtctttca gtttctggga ttgtaagtcc aaataaagtt ttatcaaatg
     3241 ctactgcaaa acctggtgat aagttaatta taacaaaacc aattggagtg ggtgtattaa
     3301 atacagccat gaaagaaggt atggtagaac aacatatagc agataaagta atagaaataa
     3361 tgatacattt aaataaatat gcagcaatga gttttgagaa atttgatgta aattcggtta
     3421 cagatataac tggttttggt ctacttggtc atactttaga aatggccaaa gcttctgaag
     3481 taagtattga aattgaatca aaacatgtac caattattga aggtgcaata gaaatggcac
     3541 aaatgggaat tatacctgct ggtatgtata aaaatatgca ttatgtatct aaagatgtag
     3601 aagttgtagg aaatatagaa gttgcagttc aagatatttt atacgaccca caaacatcag
     3661 gagggttgtt aatttcagta aaagaagaac ttgcagagga acttgtaaaa gatatgaaat
     3721 taaatggagc aatagaagct aaaattatag gttctgtagt tccaaaaggg gaaaaatata
     3781 ttacagtttt ataaaatatt atagagaaag agtgattgtt ttgagcaaaa gagaattgtt
     3841 tgcaatgttg ccttcagttg atgaagtatt aagtgataat agaattgtag aaataataaa
     3901 tgaatatcct agaagtttag ttttagaatc tgtgagagaa gtaatagatt taaaaagaca
     3961 atttatatta agattaaaag aagatgcatc aaactctgta acaatagagt ttggtgaaat
     4021 aatagaatct gcaatagaga gagttaagtt aaattactca ttatctttaa aaaaagttat
     4081 aaatgcaact ggtacagtta ttcatacaaa tttaggaagg tctttactta gtgaagatat
     4141 aaaagatgaa ttatggtgtg ctgcttcaag gtattcaaac ttagaatacg atttggataa
     4201 tggagaaaga ggttcaagat actctcattt aactagtaca ataaaaagac ttactggagc
     4261 agaagatgta ctagttgtaa ataataatgc agcggcagta cttcttgtac ttagcacaat
     4321 ggctaaagga ggagaagcaa ttgtatctcg tggagaatta gttgaagtag gtggttcatt
     4381 taggattcca agtataatgg ctcttagtgg tgcagaatta gttgaagttg gttcaactaa
     4441 taaaactcat ttgaaagatt acaaggaggc cattacagaa gatacaaatg tattgatgaa
     4501 ggttcataca agtaattata gaataatggg attcacagag agtgtttcta tagaagaatt
     4561 ggttaattta ggtaaaaaat ataaactacc tgtaatagaa gacttaggaa gtggagtttt
     4621 tatagattta tcaaaatatg gtttatctta tgaaccaact gtattggatt ctattcgaca
     4681 aggtgcagat gtagtaactt ttagtggaga taagatgctt ggaggtccac aagcaggtat
     4741 tatagttggg aaaaaagagt atatagaaaa aatgaagaaa aatcaactta ctagagcact
     4801 tagagttgat aagcttacaa tatgtgctct tgaagcaaca ttgagaatgt atttagatga
     4861 gacaaaagct atagaaaata tacctacttt aaaaatgttg acatataaaa ttgaagaatt
     4921 agaagtaaaa gcaaataaac tatttgaaaa aataactgct ttaaacttaa atgcaaacat
     4981 aaatatagaa gatggatttt cacaggtggg aggagggtct atgcctcttg aaactataag
     5041 tacaaaggta atatcaataa ctcctgaaca tatgaatgtt tcatctttag aaaaaaaact
     5101 aagacttagt gaagcacata taatagccag agtgtatgat aataaatatg tactagatgt
     5161 aagaactata tttgatgatg aatttgatgt aatagttgag gaattgagaa aagcatttaa
     5221 ttaaataaat ttgagttaat aaatattaaa tttagaagta taagtatgaa gttttaatta
     5281 aaaaatagct acacacactt gtgactttag tcatagcagg tttagagggg gcaaaaaatg
     5341 aaaaatgtag taattggaac agcaggtcat attgaccacg gaaaaactac tcttataaaa
     5401 gcattgactg gaagagagac tgatacatta gatgaagaaa aaaagagagg tatttcaata
     5461 aacttaggat ttacttattt tgatttacca aacaatagaa gagctggaat tgttgatgtc
     5521 ccaggacacg aaaaatttat aaaaaacatg ttagctggag catcaggaat tgatatagtt
     5581 ttacttgttg tagcagcaga tgaaggagtt atgccacaga cagttgagca tttagatatt
     5641 ctaagctttt taaatatcaa aaatggaata gtggttctaa ctaagtctga tactgtagat
     5701 gatgaattta gagagctagt aaaagaagat atttgtgaaa aaataaaagg aacttttctt
     5761 gaagatgctg aaataattga agttgattct atatctaaaa gaggtataga cactcttatt
     5821 gaaaaaatag ataaaatgag tgatgatata gaagataaaa atgaaaattc tccagcaaga
     5881 cttaatatag acagagtatt ttcaataaaa gggtttggta ctgtaataac aggcacttta
     5941 atagaaggaa agataagtat agaagatgat ttggttatat atccacaggt attaaaaaca
     6001 aaaataagaa gtatacaagt tcatggagaa aataaagata cagcttatgc aggtcaaaga
     6061 acagcaatca atatatcaaa tgtaaaagtg gatgatataa aaagaggaga tgttttagct
     6121 gcaccaaact cattggagga atcaatgatg ttagatgtaa aattatcatt ggtaaatcat
     6181 ttagataaag gattaaagca ctgggataga ttaagattgt atcatggaac tagagagata
     6241 ctttgtagag cagttccact agataaggaa ttaattgaaa gtggagaaag tggctatgtt
     6301 cagttgagac ttgaagaaag catagtatct aagaaaggtg atacgtttgt agtgagacgt
     6361 tattctccaa tggaaactat aggtggaggt gtaataattg acccatctcc taaaaaacat
     6421 aagaaatttg atgaaaaagt tatagaagca ttaaaaataa aagaaaaagg cgaactaaaa
     6481 gatataatag aagagtactt aaaaaggaat ttaaaaaatt atccaaatat aaaagaaata
     6541 atgagctata gtggtgctca tgaagaagat gtaaaaagag cgcttgaaac acttatatca
     6601 gaagataaag tatttattat aggaaatatg tatatgcata taaatcaata caataaatta
     6661 aaagaaaata caataaagct tttaagtgag taccataaga agtataggct tagaaaaggg
     6721 atactaaaag aagaagtaag gtctaagata gagagtaatt ttaagactag ggagatggat
     6781 attttattag aaaaattgtc tacagaaaat gctataaaaa tagaaaataa tatagtatct
     6841 ctattagatt ttgaggttat tttaaatgat aagcaaaaag aaatagcaaa aaaaatagaa
     6901 aaaagattaa aatcatgtgg agtttcttct atactgacaa tagatgaagt ttctgaagga
     6961 aatcataact atgctgaagt attggagtca atgataggta ataaagtaga gaaattagat
     7021 gatttgtata taatggacaa agatatttat gaaaatgcaa agaacatttt gataaattat
     7081 ataaaagaaa ataaagaaat aacattagga gaatacagag atttgattga ttctagcaga
     7141 aagaattgca tgattatatt ggaaaacttc gatagaaaca aaataactaa aagagtagaa
     7201 aacaaaagaa ttttgtttta gcagaaaatg tctgtttttg ctttaaggta taaagaagga
     7261 attataatgt cgagtatcgt ataattaatt agtataaaat attaattaag aggaagtgta
     7321 aagtattgaa gaatataata cctgatgtaa aaccagatat aaaagaaaga gaagacatga
     7381 gccttttatt tataataatg ggctcagtac ttttattatt tattataaaa gatatagaga
     7441 aatatgaaac tttattacat gccatatata cgatattttt agtagctaca ggagtaatta
     7501 tatttaatac tacaaaattt aaagtagatt cattttctac atttttagga ttaattttta
     7561 tagctactgg agtattggag tgtatatatt tatttaattc cttggggatt aaaactaagt
     7621 ctataatgga gattaacatc acaatatcag caataacaga tttatttcct atactgggtg
     7681 tatacttaag ttttaaattt gttaaggata acaaacaaat ttattcaagt gtaattttat
     7741 ttatagcaac tgctacatta acaataacat ctttattcat aatatcagga atttgtgact
     7801 atctgggaaa tggtaaatca caatatgctt taggagttat aatatctata ttcataataa
     7861 ttatttctat tgttagtgga attgaattaa attcaagttc aaataaaggc aaatgggaat
     7921 atgatgaaaa aaagattttt aacagaataa tagtaataat aatcttatca agagttccaa
     7981 atctattgca tatagttatt gataatagaa atgtagagag gatattatcc cagataataa
     8041 taaatgtagc cttatattat ttatataatt acatagtatc taagaatatt aagaaaacag
     8101 cactggaatt gcatgatacg aatgaagaat taacaaaaaa aacggaaagt ttaaaagaaa
     8161 agaataaaaa attaatctat gagacagaaa agatagagga acttaaggga atacttatga
     8221 aaagagaatc taggctacaa tctacattag atgtagcagt aaatagcata gtagtattta
     8281 gcaaagatgg agaaattaca tatgcaaata agagctttag aaatactttt ggagactata
     8341 aagaacaaga taaattgact gataaaataa aaaacttcaa tgatttgata gaaagcattc
     8401 attatgtgtt tgttcatgag aagaatgcag agaaacttat tagtacaagc gataataaag
     8461 tttatcaagc tatatttgca cctttaatta ttgcttcaca aacagaagga gtattgtgtg
     8521 tactagaaga taagactaag aaaaaggagt acgaacgcaa acttattgaa gcaaataaga
     8581 gaagtaaaga ttttttagaa agtattggag atggcatagt agtgcttgaa ggtaataaca
     8641 aaatatatgt taataatgca tgtagggaaa tttttaaaaa taatttagag agtattgatt
     8701 tttcattagt atgtaaaagt gaagaaagta tagaaaaaag atatgtaata gatggtgaag
     8761 taaagtatgt agagatgagt ttttctcagt atactaatgc tggtactaat aaaactatta
     8821 ttgtaatacg tgatacaacc tctcgaaaaa tatctcaaat aaaacttgag gaaagtcaat
     8881 catcatattc aagatttata gatatattac ctgatggcat atgcttatta aaagaagatt
     8941 taagtataaa ctatgcaaat aagtcgtttt tagatatgct agcttttaca aacatagatg
     9001 atataaaaga ttctaatatt aaactattaa tgaatgcgaa tactgaagaa aaaatgaaat
     9061 ttacggataa tatggctaaa gttttaaatg aaaatagaag tatactttta ctggaatatg
     9121 aattgataaa tagttatgat gatatagtag aagtagaatt aagtgcatta ccttttgcta
     9181 tatacaatac tagatacata atgcttatta taaaagattt agtacataag aaatattccg
     9241 agcaagcaga aaaagaactg cttgaaagat ttaaaacaga taaaatcaaa acggagtttt
     9301 ttgcaaatat gtctcatgaa ttaaaaactc cactaaatgt tatatctagt tcaaatcaac
     9361 tggtagattc tttctataga aatgaaaaga taaaagatta caataacaat attaaatctc
     9421 atgtggattt agttagacaa agttcttata ggcttcaaag attaataaat aacattatag
     9481 atttaacaaa gatggagtct gggttctata cactaaaact tgcgaaatac aatattgtat
     9541 ctgtaataga agacttattt atgaatatag aagagtatgc acttagaaaa gacataaaaa
     9601 ttctttttga tacagacctt gaagaaatta atgtttatat agacaaggtt gaaattgaga
     9661 gaattatgtt gaatctattg tcaaactgta ttaagtttac tgataatgga ggctggatat
     9721 atgtaagtat tcattataaa atagataaag taatcataag tgtaaaagac actggagttg
     9781 gaataccaca agataaatta gaattgattt ttgaagaatt ttcacaagtt gataagactt
     9841 tatccagaaa tactgaagga agtggaatag ggcttgctat agttaagaat ctagtaagtc
     9901 ttcatggtgg agatatagaa gtagtaagtg aagtgaataa aggcacagag ttcttaattt
     9961 cacttccaat gaagggattt agtagtgaac attacacaga agataagaga atatataata
    10021 ttcaagaaaa aataaaaata gaattttctg atatatacta ttaaaaatga ttatgagcaa
    10081 aaataaaata aaaatactat ttgtttaaat atttaagttg taagaaatag gctatcataa
    10141 ggtaaattat tatgatagcc tatttttagc taggttttat atctatatgt gagaacatat
    10201 aatctctaaa ttaccttcaa aactaaaatc tccaaagtcg tttggtagta taaaatgcat
    10261 accctttttt aaattatatt ctacagaatt gtgtataagt tttccatttc catccaacac
    10321 actgtataaa gaaaaaggtg tattgtgtgt aaaattacat tttccaaaaa catctaattt
    10381 ataaacacta aaaaattcgt ttgacacaaa tgttgtgcat ttaaagtctg atgttgatac
    10441 gattttatag tcagtatcga aatttatatg aggaacattc gtaacatcta ttgatttttg
    10501 aacatgaagt tcacgcttat tacctgaatt gtcagttcta tcataatcat agactctata
    10561 agtagtatct gagttttgtt gagtttctaa aatcaatgtt cctttgcata tagcatgtat
    10621 agtaccactt ggaacataga aaaagtctcc cttttttatg ttaacctttc taagtagttt
    10681 gtcccattca ttattattta ccatatttac aaattcttta tgagattttg cattatgacc
    10741 tattataatt tctgcatcat cagaacaatc tattacatac caacactctg tcttacctaa
    10801 ctctccattt tcattttttt tagcatattc atcatttgga tgaacttgaa ctgataaatt
    10861 atcatttgca tccaatattt ttgtaagtaa aggaaactta tcaccaggtg tgtttccaaa
    10921 taattctcta tttttattcc acaattcaga tagtttttta cctttatatt ttccattttc
    10981 tattaagcaa tctccatttt tatgagagct tatagcccaa cattcaccag ttgttggcga
    11041 atctatttca taattaaatt tatctttaag tgcagtacca ccccatattc tatccataaa
    11101 aataggtttt aaaaataatg gttccataat tctgttcctc ctatgatatt aaatttatcc
    11161 tacaaataat tatatataaa tagattataa aatcaatagt aaaaaagttt tgtcccttga
    11221 aatgacataa agtataagga gttattaaat aagataaact atgatatata atataaagtt
    11281 ctaaattgga gtgataatta tggagttgtt taaggcagaa agcaaaaaat atgaaaaaga
    11341 atttttaaaa ttattggagc aatgggttag tataccttca ttttatgata gaaaaactgt
    11401 atctaaagat atgccttttg gaaaaggagt atatgatgca ttaaactggt ttgaaaatct
    11461 aggtagagaa aacaatttca aagtaaaaaa tatagataat catgctgttc aaattgaata
    11521 tggaaatggt aaagagtatg tagatatatt tggacattgt gatgttgtaa atccaggtga
    11581 aggatgggat agtgaaccat ttaaactaaa tattataggt gataaattag ttgctagagg
    11641 tgtatctgat aataaagggc ctatgatagt gaatttttta gcactaaaaa tgataaagga
    11701 tttagatatt aatttaaaaa gaaaggttag attaatagct gggggaaatg aagagagtgg
    11761 atttaaatgt ataaagcatt attatagcaa agagccatat ggtgtatgtg gatttactcc
    11821 agatgctaaa tttcctgtat taaatggaga aaaaggtgga gcaataataa agttaatttc
    11881 aaatatagac gataaatctt tatatataag tggtggaata gagtttaata caataccaga
    11941 taaagtttat ataaagaatg tagaaaaatt gggtaaagat aatatatgtt ttgatattaa
    12001 caatatatcg ataaattatg ataatggcaa ttatattgtg tatggaaaag gtggtcattc
    12061 atctaaaccc gaaaaatcaa taaatcctat attagctaca attaaacttt tgtcagaaaa
    12121 tattgatgaa aaatggacaa aagatttata taagttgata aatcaagata atataaatgg
    12181 aaacttattt ggtttaaata ttgaggggaa atgtgggatt ttatcaatgg tgccaacaat
    12241 aataaatata gtagatggaa aattagaagt agttttaagc gttagatatc cagaaatatt
    12301 aaccattgaa gatataataa aaaaatttaa tttgtatatg gagcaaaata atattaataa
    12361 atttgagctt attggggaaa atttaaagca agcaaattat atagatagaa attcaaaact
    12421 tgtaagaagt ttgcatgata tatatataaa gtattctgga gatttaaaaa atgatgttag
    12481 agtaactagt gcaggaagtt atgcttctga aatgaataat tcagtaatat ttggttgtga
    12541 atttcctgat ggtagctttg gcaatgtcca tagtgcgaat gaatttgcta gcttagatag
    12601 atttataact gcaataggta tatatgcaga ggcaataatt acattatgta ataaaatata
    12661 aatacaaaat gtcccttata tagacatatt atatatacaa ttaaaatttt acttgatata
    12721 taattgaaat caaagagaag gtgatacaat gataactaag aaacatgcaa taatagtaaa
    12781 gagtttaagc aataaagatg gatatatgac atcaaacgaa ttagctatca aattggatgt
    12841 gtctactaaa actattaaaa gatatatagc agatttaaat agtgttttaa gcaagtatga
    12901 tttggtaata gcttcttcta gaggtatagg atacaagtta agcggttcta aaaacaatat
    12961 agccagagct attaaagaag caaataaata tattgatggt tttttagatg actctgaaga
    13021 gtcaagaatg tcaaatataa tatgtatgct tattaataga aattatatga gtatagaagc
    13081 aatggcagaa gaattaaatc taagtatagc cgctataaat aaattgtcta gtaagcttaa
    13141 aagaaaactt gaaaagtatg acttagttat aaaatctaaa ccttactatg gaagctatat
    13201 acatggtgaa gaaattaata taaggcaatt aattactgac tatgcaataa aattagatga
    13261 aaaaagtaaa gtgaaggtgt ttttagatga cattagtgaa aacgatatac actgtataga
    13321 aagtatttta gaaaagcact taagagaaaa agatacaata atttcagata aagattttaa
    13381 tcttttgctt tcaaaaataa tcgtttctgt atttaggagt aagagaggtc atagtaacaa
    13441 tataaactta atggatacaa gttatagatt tcataactac tactttatag aaaatctaat
    13501 gaaagaaata tctgataaaa ttggatttaa acttattgaa gatgaagtaa tttatatttc
    13561 aaattattct ggtgtaatag cctataaagg tacacaagga agaaaaaaca caagtgaaat
    13621 tgaggaacga ataagtagtg ttataagtag tgcacttcaa gatatattcc ttatttcagg
    13681 tagtgattat acaaaagatg atgagtttat ggttgcaatc tctgaccata taaagagatt
    13741 tttaaataga gctagagcta atgtaaaatc aaataatcct ttgcttcatc aaataaaaga
    13801 gaagtttcct atagctttta atttagccgt gtttttatca aataaactag aaactgagtt
    13861 taatttaaaa cttgatgaag atgagcttgg atatatagct atccactttg ctgcttcaaa
    13921 tgaaagaatg aaaaaaaata ctagtaagaa aatttgtata gtttgtcatt atgggatagg
    13981 aacaggacaa cttatttctg aaaagctaaa acagaatata agtgatttaa gtgttgtggg
    14041 tgtttatcca gttagatatc tcgatatggc tataagtcaa gatgttgatt tgatagtatc
    14101 taccgttgaa ttaaaaggat atgaaaaacc tgtcttatat atagaaaata tatttgatga
    14161 tagcttaatt gaaaacgtaa acaaagcctt ttatgaaaag gaagaaagaa gaaagataat
    14221 aagcaacatg tttgatgaaa aagctttttt cagtataaag gctagtacta aagaagaggt
    14281 cataatttca ctttcaaata aactaaaaga aagagatttt atagaggaaa gttctataaa
    14341 aagtataata gatagagaaa atatttcatc aactgaaata ggaaatctag tagcaatacc
    14401 ccacacaata gtgaagggag ataaaaaatc tataatagga gttggaatac ttgaaaatcc
    14461 aataatttgg gataagcaag aggttcagct agtatttatg gtatttttta atactaaaga
    14521 aaaacataat ttctcgatat ttaaatacct ttataatttt ataaaagatg aaggcggagt
    14581 aagaggaatt attaaaatat gtgactttaa taaattaatg gcactgatag gtaattaagg
    14641 aggaaattaa taatgggaac aaacatattt aacaaagaat atgtattttt aaatgttgat
    14701 gctaagtcaa aggttgaagt tttaaaattt atatcaaaaa aagctaaaga cttaaacttg
    14761 gcagaagatg agagcttagt atatgaaggt ttaatggcta gagaagaaca atttactaca
    14821 aatttaggag aatctatagc aatacctcac actaaaaatg atgcaattga aaatccagca
    14881 gtagtagtat tgaagtttaa tgaagatgtt gtttggaatg aaggtgagga taaggttaaa
    14941 ttagcaataa gcttacttat gccaggaaaa agtaaagaga atattcatct taaattatta
    15001 tcatctcttt caagaaaatt aataaataaa gaatttaaag attctctttt aaaaagtgat
    15061 aatgttgaag aaatatctaa ctcaataaat gaagctttag gtttataatt aaacactaag
    15121 gttataaaat aaaaaaataa ttaactaaaa ataggaggaa tttgaaatga ataaaaaatt
    15181 agtagcatta tgtgcttgtc caatgggact agctcacact ttcatggcag cagaagcaat
    15241 agagcaagca gcaaaggcat taggttatga agcaaaagtt gaaactcaag gtgctgatgg
    15301 tgtacaaaat gagttaacaa gagatgatat attaggagca actatgataa tacatgcagt
    15361 agcaataaca ccagaaggta tggaaagatt tgatggttgt gaagtttatg aagttgaact
    15421 acaagaagct ataaaaaatg cagaaggtgt tataaaagaa atagaagaag atttaggaat
    15481 ataataaatt tattgtggag gaaatagaat ggcaattaag aaaaaagtta taaacagttc
    15541 aggtgcttct acaacaggag gaaataatgt aggaaaatct actccatcta gtccaactca
    15601 atcaaaggga aatggaaaat ggaaagaaat aagtaaacat ataatgacag gtatatcata
    15661 tatgatacca gttttagtaa tgggtggact tataggggcc ttatctcaat taataccata
    15721 tgctatatta ggacttgacc catctgtagg tatagttgat gctatgaact caggagaatt
    15781 tacagggttc aagcttagtc ttttaaatat agcacaatta atgtcaaatt tcggatttac
    15841 tttgtttgga tttgcgatac cactgtttgc agcattttgt gcaaactcta taggtggaaa
    15901 aacagcttta attgcaggat ttataggtgg atatatagct aataaacctg taggtgtacc
    15961 acaattcgtt gatggacaat ggactgaagt tgttccagtt gcatcaggat ttttaggtgc
    16021 aatactaata gcatttataa taggatattt tgtaaaatgg ttaaataaat ctataaaagt
    16081 ttctcataac tggttagctt ttaaaacaac atttttaata ccattaatag cgtcattagc
    16141 ttgtatggta ttgatgatat ttataataac tccatttggt ggattaatta atgaaagtat
    16201 gaaaaacttc ttaacagcag caggagcagc tggagaatat gtatatgcaa cagctttggc
    16261 agcagctaca gcattcgact taggaggacc aataaataaa gcagcaggat tcgttgcact
    16321 tggtttaact acagagaatg tacttccaat aacagcaaga acaatagcta tagttgttcc
    16381 tccaattgga ttaggattaa caacattatt agataaaaga ttagtaggaa gaagagtata
    16441 tgatagacaa ttctatcaag cgggaaaaac ttctatattc ttatcattta tgggtatatc
    16501 tgaaggagca ataccatttg cacttgagag accaggtttt gttatacctt taaacatagt
    16561 tggaagtgta ataggagcta ttacaggtat aattctagga gcaattcaat ggttcccaga
    16621 gtcagctata tgggcatggc ctctagtaga taacttattt ggatatataa taggtatagc
    16681 agttggtgct atattcatag cagtaggaaa tatattctat agaaataaat taataaaaga
    16741 tggtaaactt gttgtagatt atatcgacta gtatttaatt tttactatct ataaataact
    16801 ttggctggta atatggtttt aatcaatatt accagcctta cctatatgat taattaaagg
    16861 cggtggagat aagatatgga taagatagaa aaagtaagag agtattttag agaatatgat
    16921 atagatggat ttcttataaa tagtcctaca aacaagtttt atgttggaaa tttatttagc
    16981 tcttcaggtt atgtttttat aacgaaagaa tcacaatata taatagtaga ttttagatat
    17041 tttgaagaaa ttaaaagaaa atctagtttg tttaatgtag tattaatgga caaaactaga
    17101 actcattttg atataattaa tgatatttgt agagagcaga atataaagga aattggattt
    17161 gagggaaatg aagtatcttt tgatttatat agaagtatga gtaataagtt aagtgcaacc
    17221 ttaaaatcag ttgatttatc tacattaagg gaaacaaaaa atgaagacga aataaaatat
    17281 ataaaaaaag cttgtgaaat agttgatgct acattttatc atatagtaga ctttataaag
    17341 gttggtatga ctgaaaagca agttgaaaat gaaattgtta gaattataaa agctttaggt
    17401 ggtcaaaaag aatctttcga tacaatagtt gcatctgggc ttagaggagc attacctcat
    17461 ggaaaagcaa gtgaaaaagt tatagagtat ggagattttg ttacttttga ctttggtgct
    17521 aagtataata attattgttc agatattacc agaaccattt gtatgggtac tataaataaa
    17581 gagttagaag aaatatataa tatagtaaga aaagctaatg aagagtgtat aagggtttta
    17641 agaccgggaa tgactactgg agagatagat aaagttgcaa gagacataat tggttcatat
    17701 ggatatgcca ataattttgg tcataatctg ggtcatggtg taggaattat ggttcatgaa
    17761 tatcctgcat tagctcctga atcaaatgaa gtattaaaag aaggaatgat tgtaactata
    17821 gagcctggta tatatgttcc aagtcttgga ggcgtaagaa tagaagatga tgtacttata
    17881 acacaagatg gatgtatgag attgactaca tctactaagg atttaatagt tgtaagctaa
    17941 aaataaatta gataaatatt ttataacaat aagaaaagaa gaggtaaatt taaatgtata
    18001 attttgatga taaaccagat agagtaagtg aaaagtgtag aaaatgggat ttaaatatta
    18061 taagagataa atttggagat attagagaag attttgtacc aatgtggatt gctgatatgg
    18121 attttaaaat accaacagaa atagaaaaca aatttattga agctgtaaga agaggtgtat
    18181 ttggatatac atactgttat gatgaatttt atgattcagt tattggttgg caaaaaaata
    18241 tgcatgaggt aaatgtagaa aaagaatgga ttacattaac ttatggaact gtatcaacac
    18301 ttcattatgt agtacaagct ttttgtaaga aaggtgatag tataatatta aacacaccag
    18361 tttatgaccc ctttgaatca tcagcaaaaa aacagggtgt aaatgtaatt tgtaatacac
    18421 tagatgttgt aaatgataga tattatataa attttgataa attagaagtt caaattaaag
    18481 aaaataagcc taaattaatg atgttttgta caccacataa tccatccgga aggatatgga
    18541 caattgaaga aatgacaaga gtagcaacta tatgtaaaga aaataatgtt attttggttg
    18601 cagatgaagt acatgcagag catattcatt atggtaaatt taattcaata cttaagattg
    18661 gaaaagagct tttagaaaat gttattctac tgacatcacc aaataaagga tttaatcttg
    18721 gtggattaaa gacttcttat tcaatagtta taaataagga tataagggac aaatttagaa
    18781 ataaattaaa gcaaaattct ataacttctc caaatgtgtt tggaataata ggattgataa
    18841 ccgcttataa tgaatgtcat gagtggttaa gtggtgtaaa tgaatatata aaatcaaatt
    18901 atgaactttt agaaacttgg gtaaataaat ataataagat aaaattgatg aaaatggaat
    18961 catcttactt agcttggatg gatataagtg gattaggtat tagtgcaagt gagtttacag
    19021 ataaattggc tatagataca ggagtattat tagaagatgg aagtcatttt gtaaaagatg
    19081 gtgagaagta tgttagaata aatttaggaa ctcaaaagga aaatgtaatt gaagccttaa
    19141 atagaatgga tttattttta aagtctttat aaaaatttgg ttgaattagt attttat
//
LOCUS       DAKBNN010000061        18904 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000061 DAKBNN010000000
VERSION     DAKBNN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.061"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            39..635
                     /locus_tag="OUB02_001800"
     CDS             39..635
                     /locus_tag="OUB02_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HCT8885560.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(733..1692)
                     /locus_tag="OUB02_001801"
     CDS             complement(733..1692)
                     /locus_tag="OUB02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8885561.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1726..2457)
                     /locus_tag="OUB02_001802"
     CDS             complement(1726..2457)
                     /locus_tag="OUB02_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885562.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2450..3220)
                     /locus_tag="OUB02_001803"
     CDS             complement(2450..3220)
                     /locus_tag="OUB02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885563.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3305..4015)
                     /locus_tag="OUB02_001804"
     CDS             complement(3305..4015)
                     /locus_tag="OUB02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HCT8885564.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4195..4347)
                     /locus_tag="OUB02_001805"
     CDS             complement(4195..4347)
                     /locus_tag="OUB02_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCT8885565.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4373..6529)
                     /gene="feoB"
                     /locus_tag="OUB02_001806"
     CDS             complement(4373..6529)
                     /gene="feoB"
                     /locus_tag="OUB02_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCT8885566.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6629..6850)
                     /locus_tag="OUB02_001807"
     CDS             complement(6629..6850)
                     /locus_tag="OUB02_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCT8885567.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6863..7075)
                     /locus_tag="OUB02_001808"
     CDS             complement(6863..7075)
                     /locus_tag="OUB02_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCT8885568.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7396..10293)
                     /locus_tag="OUB02_001809"
     CDS             complement(7396..10293)
                     /locus_tag="OUB02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8885569.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTEADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10620..11228)
                     /locus_tag="OUB02_001810"
     CDS             complement(10620..11228)
                     /locus_tag="OUB02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCT8885570.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11488..12033)
                     /locus_tag="OUB02_001811"
     CDS             complement(11488..12033)
                     /locus_tag="OUB02_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HCT8885571.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12151..13974)
                     /locus_tag="OUB02_001812"
     CDS             complement(12151..13974)
                     /locus_tag="OUB02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885572.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(13987..15762)
                     /locus_tag="OUB02_001813"
     CDS             complement(13987..15762)
                     /locus_tag="OUB02_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885573.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            16055..18283
                     /locus_tag="OUB02_001814"
     CDS             16055..18283
                     /locus_tag="OUB02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCT8885574.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNNTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(18521..18820)
                     /locus_tag="OUB02_001815"
     CDS             complement(18521..18820)
                     /locus_tag="OUB02_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCT8885575.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
BASE COUNT         6400 a         3242 c         1963 g         7299 t
ORIGIN      
        1 acttgatatt aattttcatt tgaaggaggt tatcttttat gtcttgttgt aattcacaat
       61 tttgttgtaa aaataaaatt gaatcatcat atataaaatg gcttttagaa gttttaggtc
      121 cagtcttttt aggctctaaa cctgctgaaa taataaatat ttcgtttact gatgtaaata
      181 gagaggaaaa aattaatgat atttataaat atctatataa atgtaagaaa atagatttta
      241 tagttattga taaagaaaaa aagggattaa aaatcttatt tgtaaataaa aaagcattat
      301 cagaaaaatt aaaatgtaaa aaaacagtaa actttttaaa gtttttagga tacaaacaaa
      361 atacgaatgt taatgcttat ttagagcatt tagtagataa acttaagagt gatgtttttc
      421 ctgatgaaat aggtattttt ttaggatatc ctcttaaaga tgtgattgga tttatgggat
      481 atagtaatta tgaagtttct atgattaaat attggaaagt ttatggtgac acaaaacaat
      541 ccgaggacac atattcgaag tttttacttc accgtaagaa aatgagaaaa ttactagact
      601 acataagtgt agataaaata gtaagctgtt tttaattgtg tttttgtata ataataaaaa
      661 tacaccctct tttaccatac agtttccata ataaataagg gtgtattttc cactttaata
      721 ttttaaagta ttctaatttt gattgtacaa catatcttta atatttattt tcttttgttg
      781 cattccatta gacatcaaaa attcctgtgt atcttctaat tcttttatat ccttgtccgt
      841 tattttagaa ctaaagtcat accaagaata catttctttt gtttcatcta aagataaacc
      901 aacttcttta gatacaacat ccatcacttc atccttgttt tcattcatat attttaaagt
      961 ttcttcatga accttcataa atctttttac taattcagga tatttttcag caaagtcatc
     1021 acttacagca gtaactatta ttccacttac taatccttct ccatttacaa tcattttggc
     1081 acctgaattt atcgttttca atgccactgg acctgcaagc attgctacat cagcactttt
     1141 actttctaat gcagaagcag cactaggaat atccatattt acatattcaa catcatctat
     1201 agtatagcct tctttagcaa gtgcagcaat caacagttga tgaagtatag tcccttttgg
     1261 acctacaatt tttttacctt ttaagtcttt tatagattta atattatcat tattagttat
     1321 aagcatatat ccttttggtg aacggctata aatattcgta attttaagac caacaccatt
     1381 tgatgcagct attattgcag acgttcctcc taatgcatgt aaaaagtcta gctcacctgc
     1441 tgctaatgct tgagtttgct ctggtcctgt tgtcaattca tggaaattga cttttatatt
     1501 gtccttttta aattcctttc caaataaatc atcctttttt tctaatatac taggaacatt
     1561 aagtggtgat tttacataag ttaaatttat ttccttaggt aaactagcct ctttttttgc
     1621 aggttcatct ttcttatcat ttgttgttga acatcctaca gtcccaacca acaccattat
     1681 taaagatgtc attactgcta aaatcttttt gtttctttta atcaattaaa actcccccaa
     1741 ttcccttaat atttcttttt tcaagtttat ataatagttt ttagttaaat ctctattata
     1801 ttcatcttcg acactaaatt gtttaatcct tttattttta ctaaaaacaa ttattttctt
     1861 tgctatttcc atagcttctt caatattgtg agttacaaat actacacctt ttttagtatt
     1921 tttatggata tttacaactt ctttttgcat ttttcttctt gtgaagtaat ctagtgctgc
     1981 aaatggttcg tccatcagca aaatatcggg attgaaggaa agtgctcttg ctatacttac
     2041 tctgtgagcc attcctcctg ataattcatt tggatatgag tttttaaact tttcaagctt
     2101 catcattttt aaatacttgt ctaaatcaac tttatttcta ttatcttttt cagtatgtaa
     2161 aagtatattt tcacttacat tcagccatgg cataagtcta ctttcttgaa ataccattcc
     2221 tacttttgga gtacactttt tatcattatt aaaaaaatat atgtttccac tagtagcatt
     2281 ttcaagccct gctaaaattc taagtagcgt tgttttccca catccacttt cacctaatat
     2341 aacagttatc tcttcactag atatatttaa agatatatta tcaagaacta aatgctcttt
     2401 attatcaact aaatatttct tatatatatt ctctagcaaa aatccacttc taaccattta
     2461 aatcaacctg cttccctttg ctaaattttg ttattatctt tgaaaataga taatctgaaa
     2521 gtatacctaa aaatccaata acaaaaattc ctaccaatac aacatcagtt ctagacatat
     2581 cttttccatc agaaatcaaa tatcctagtc ctgaagaagc tgcaataagc tctgctccaa
     2641 ttatagccct aaaactatac ccaagcccta aactaagtcc aacaacaata tccagcatag
     2701 aattagggat tattatttta taaaaaattc tctttttaga taaattaaaa gatttaccta
     2761 cttctattaa tttattgtca caatttttta tacctttcaa tgtatttaaa aatattggaa
     2821 aaaaggatgc caaaataata attataattt tagattcttc acctatacca aaccataaaa
     2881 taagcattgg caccaaagct agtggaggtg tacttcgcat aaattcaaat agtggtttga
     2941 aatattcata cattttttta tttactccaa ataaaactcc aagtggtata gccatcaatg
     3001 aacttattaa aaaacctatt aatatccttc ttacactggc ataaacattt aagaaaatag
     3061 aaccgtcata tatcattttt ataaaagtat ctaagactat ttcaggagga ggcagaatat
     3121 agctactcca tatacccaaa acacatacta tcttccataa aattaaaatt attaaaaaaa
     3181 tataatatcc tttaagcctt ttatgaattt tctcaaacat ataaaactcc ttttaacata
     3241 tctatccctg ctaccctttt tattttttaa tatcttaaat aataattaag ctatatcata
     3301 agatttaatc tatgccaagt cttaatccac ctgaactcat tctatcagaa ggtgtagaat
     3361 ctgtttttaa gccagacaat ctttcagatg gttgtataat gactatttgt ccaggttcaa
     3421 caaattccat atgataagat tctcctgaag tctgaccaat aaaagtcttc catgatagtt
     3481 ttgatacttt aggatatggt tctcttccag tccaagctac taatgcatct gggtcaacac
     3541 aacaaggtcc ttcaagaatt agaggattac catctgttat tatagcaaca tcttgatttt
     3601 gacctgtttt atttactaaa tgtgtagtaa aaagaccttt ttgagatatt acccctgaac
     3661 caatcaattt tacactgtaa tttaattgtt cagaaaatgc caataagttt tcactctcaa
     3721 cataaatgga atcacctggg tcaattgata ttacatctac atggtaagca ttttgagcta
     3781 ggtaaatttc accttcacct tcaaccacca ttattggcat ttgttctcct gtcaatgacc
     3841 tctgaacatg acctaataat gctcttccca atccaccgcc attacttgga cctaacagca
     3901 atttttcaaa attaaaattt cctttaaagg ctaccatagc accttttttt gcaaaaaatt
     3961 taccacttcc ccttgctata cacataagct cgtttgtaac atcaaattta aacataatat
     4021 tcacttatat tgcttaattt agcaataatc cctcctaatc atatatttat atataattat
     4081 acagtaaaat tacagcatag tctaacctac ttcaaatatg aaatttgtta tttatacaaa
     4141 aaataagaac ctgataataa agcaatttaa ctttttatca ggctcttatc ctatttatct
     4201 cttagcgcta tgacaactct tactacttga gcatccagaa caaccacatc cacaactgct
     4261 accattcttt tttgaatttt taaccatata cattacagct agtgccatta atactactac
     4321 aatagctgct ataataaatg tagccatatt catcactcct tatttatatg aattaatttt
     4381 tcatataact taatttaccc attttttctt ttttcatatt tttatttgaa taagttaaaa
     4441 ctacaaagac tactgctact gctataaata tagatattat tgccccagca atacttcctc
     4501 cagtacctaa aactaaactt ccaacttgat ttataagaag agcaattata tacgctgtta
     4561 aagtttggaa tcctaatgta atccatgtcc atttccatga acccatttct ctttttatgg
     4621 ctccaatagc tgcaaaacaa ggtgcacaaa gcatattaaa tgccataaat gcaaacgcac
     4681 tagctactgt aaacatgctt gcaactgaag caagaagtgt tgggtcttgc tctgttgcat
     4741 ctgatatacc aaataaaact ccaaatgttc caactacatt ttccttagct actagacctg
     4801 taactgttgc tactgatgat tgccaatttc caaatccaag aggagcaaat attggtgcaa
     4861 ctatatttcc taagctagct aatatactat ctccagcatc taccatttgt aatgaccaat
     4921 taaatgattg taagaaccag attactccac aagcaacaaa tataattgtt cctgctttga
     4981 ttataaatgc ttttcctcta tcccacatat gtattaaaac accttttgca cttggaatat
     5041 gatattgagg taattccata acaaatggag atggttctcc tgcaaataaa ctagttttct
     5101 ttaatataat tccacaaatg attatcattg caatacctaa gaaatacatt gatggagcta
     5161 cccatgaagc acctccaaat aatgcccctg caaataatgc tattattgga atcttagctc
     5221 cacatggaat gaatgtagtt aacataatag tcatttttct atctctatca ttctctatag
     5281 tacgagttga cataactcct ggaactccac aacctgaact tataagcatt ggtataaatg
     5341 attttcctga aagaccaaac ttacggaaaa ttctatccat tataaaagct acacgtgaca
     5401 tgtacccaca atcttctaat attgataata ataagaataa aagcatgatt tgtggtacaa
     5461 atcctagtac tgctccaact ccaccaatta gaccatcaac tacaagtcct tgtaaccact
     5521 ctgctacatt taaagaagct agaaaattac ttacattgcc ttgaataatt tctccaaaca
     5581 gtacatcatt tgtccaatca gttgctatag tacccaatga acttactgct atatagtaaa
     5641 ctccccacat tataagtgca aaaataggta atgctagtat acgatttgtt acaatcttat
     5701 ctatcttatc tgatacagtt tccttacctt ttcgattctt ctttattata cttgaaatta
     5761 ttgaactaat aaattcataa cgattggctg ttattatact ttcactatca tcatccaatt
     5821 catcttcaca atttctagtt atttcttcaa tactattcaa tattgtttta gaaatatcaa
     5881 gtttttgtat aacattttca tctctctcaa atagctttat agctaaccag cgtgtttcta
     5941 catctatata tggagtcttt tcttctatta ttttttcaat tttttctatt gcatccttac
     6001 tttcatcaac aaagggtaat ttgaaattta atttattgtt actacttgca agttcaactg
     6061 ccttttcagc agcctccata attccttgac ctttaactgc tgttacttct accactgggc
     6121 aacctataac ttcagatagt tctttaatat ttatcttatc accattctta tttacaatat
     6181 ccatcatatt aagagctata acggtcggta tacctagctc taaaacttga gttgtaagat
     6241 ataaatttct ctctatattt gatgcatcta caatatttat tactgcatcc ggcttatcat
     6301 ctagcataaa attacgtgta actacttctt ctaatgtata tggagataaa gaataaatac
     6361 ctggtaaatc tactatttcc acatctttat ttccttttaa ttttccacct tttttttcaa
     6421 ctgttactcc tggccagtta ccaacatatt gtgatgatcc tgtaagccca ttgaacattg
     6481 ttgtcttacc acagttagga tttcctatga gacctatttt tattgacatt tccattccct
     6541 ccattattat ttaaatacat cactgtttgt gatacagaaa atcattctca ttatataatt
     6601 acattaaccc tactctaatt aatctttatt attccacaat aattttttct gcatctgact
     6661 tacgaattga tagttcataa tctctgactg ttacctcaat tgggtcacca agaggagcta
     6721 cttttcttat aaatatatta acacctcttg taatccccat atccattata cgtcttcgtg
     6781 tagctccttc tccctctaac tttttgactt ttacagtctc tccacactta atatccttca
     6841 gtctgttcat gtcattacct ccctatatta ttattctact tgccattttt ttatctaatg
     6901 caactctagt atcttttacg tctattatta agttaccatc tatcttagaa actattttaa
     6961 catttgtacc cactacaaaa cctatactat ttaaaaaagt cctcgtctta tcacttcccg
     7021 aaattttctt tataactact tcttcacctg tatttgataa tatcaatggc atcatataat
     7081 atccctcctc ttaaagtaat ttaaatctac aagcaatcgt tatcttatat ataaattaca
     7141 ataccttaaa tcttaatcta ttaaaataac atcctatttt ttcctccttt ttacaattcc
     7201 taattttgat ttaagtctat cacaatagta tattttttgc aagtgattat tgtattcttt
     7261 tgattttttt tctcatttga tttattttat taataaaatc gtaaaaatta atacaaaaat
     7321 aaagatgctc taaaatagta tactcaacta atattaaagc acctttatta tatatgtcat
     7381 tatttacaca ttgctttatt cttttttaaa agcgattttt tcaaactctt ttacaggcat
     7441 tggcttagaa aacaaatatc cttgtaccat atcacaccca atttgcttta gaaattctac
     7501 ttgcgaaata gtctctacac cttctgatat tactttcatt ttcagttcct ttgccatttt
     7561 aatgacatta gatataacaa ttttttcatt attactctca tctttttctg taaaaaatgc
     7621 tctatctagt tttaatatgt caacaggcaa ttcttttaaa agattaagag aggaatatcc
     7681 tgtaccaaaa tcgtccatag agattacaaa tcccttttct ttcaaattgt tcattgtatc
     7741 aagtaatcct tctatattat ctaatactgc tgtttctgtt agctctaatt ctataagtct
     7801 tgtcggaatt ttgtatttat ctacaattaa tttaaactcc tctataaaat tactatttac
     7861 aaaatgcatt cgagacaagt ttacagatat aggtactact ttttgtcctt catccatcca
     7921 ttctctaatc tttttacaca cttcctcaaa tacatagaaa tcaatattta caataaaacc
     7981 attcctctca aatacaggaa taaattctat tggtggtatt agaccttttt gtggattatc
     8041 ccaacgaaca agagcctctg cccctataat ttgataatct gaaaggctat atttaggttg
     8101 aaaataaaca ataaattctc cattattaag agcatctacc attgaatttt caatttcttt
     8161 ctcttttaat atttttttgt gcatttcctt atcgtaaaaa gcaaatgaat ttttatgacc
     8221 accttttata gtttttcttg cagtattagc tctatccata atagtattta tgtcattatc
     8281 acttttactt attttatata taccacaatc taaaatcaat ttataatagt ttattttagg
     8341 attactaagt atttgaacct tattgtaaat attgtttaac cttgttttaa tatcatcatc
     8401 tgtcttatac ttaataagaa gaacaaaatg gtcagcagaa actcttgcaa aaatttcatc
     8461 ttcctttaat tcacttgcaa tagtatttga tatatgtatc aaagtatcat ttcccatatc
     8521 atatccaaac atatcattta tatatttaaa tttgtcaaca tctatataaa acaacgcata
     8581 ttcttctgga ttattcttag caaacagttt attagaattt attttaaatt tatctatact
     8641 atctgcttct gtcaatgggt ctgtatatgc tatcttcctt agataatcct ctttcttctt
     8701 tttattataa attatatatt taatagcaat taagaataaa atacatacaa aagaaataat
     8761 ttgtattgaa tattctttaa aaatcacacc aaatggtact ttataaggtt tgtcaatagt
     8821 attagaatat atacaatcac ttaattcttc ttcagtaatt ttgtctattg atttatttaa
     8881 aatagacact aatatagggt cagttttatt tgatacccct atacgcatcc catagcttgt
     8941 atctaaaaca gatataacag ataaattctc actctttcct tctcttaaaa gttcatcaaa
     9001 ctgataagaa ctaagtatca ttaagtttgc tttaccttca tttatagcgt ttagacaact
     9061 ttctacactc ttataagatg ttgtcttagc attttcaaaa ctcttttcta tataagaatc
     9121 aatagactta taactactag ttaaagcaat atttaaatca ttattgagat tggtatccaa
     9181 ttttcctacc ataatcattg gaatatttat gtatggattt gtcaatttca tattaaatcg
     9241 ttttgcctta tcattttcat tgattgagcc acatagcata gttgccttac cactttttat
     9301 gtaatcaaga ctctcattat aattttttgt tttaatatat tcaaatttta taccacattt
     9361 ttttgatata attgacatta aatcggaact tataccttta aatgaattgc tttctttatc
     9421 ataatactct acaggagacc actctgaatc atataccata gtaagttttg ggtttgcttt
     9481 aataaaattc atttcttgcc gagtaaatgc tatggaatta ttgacctctc ttttgaaata
     9541 ttttttatat aattccaact cataatacat atcatttatt tttaattctt ttaaagcata
     9601 atctaattct tttaccaaat taggtttatc ttttgcagta gcaaaataaa aaggttctac
     9661 tgaaaatttg ctaactattt tcattccttt agcatttata atacttccac aaacaaatgc
     9721 atctgccttt ttttcattga cggactcaat taattcatca atagaattac agtatatata
     9781 ttcaactgaa aaattatttt cttctctata cttctcaaat gcagtattat gaaagtttcc
     9841 tctaagtaca gctactctca ttcccttaaa ggaacttata tcttcataaa acatattatt
     9901 gttactatcc tttgtatata gtgcactata ttcatatcct gaacttagtt taggaaaatt
     9961 aaatagttta tttctttcat cattcttctg taaaggtccg agtaagtcta tttcaccctt
    10021 ttctagtctg tctaaacatt cttcccaaga cccttttaca aattcatatt cccatcttgt
    10081 atattttgag atttctctta gataatcata accatatcct gtataagaat gctcttttga
    10141 tgaggatatt attccgtagt catcacaata tccaactctt attttttttg aattttcaga
    10201 ctcatcagca aagcatagta tattgttaaa acatgatata ataagtaaaa atatcaaaga
    10261 aagtatcaag aagtgctttt taaatgcttt catatttaaa aacctccaat ttgccaactt
    10321 actttaactt atttcgtttt ctagttgtaa ctacttttat tgtaatggta aatatatcaa
    10381 attgcaatat tctcatataa tattcttatt caactataat gaatttatta ttatctattt
    10441 aaatacacta tctaatatta gtttcttata agtcataaaa gcacaactaa cactataatt
    10501 ctagtgatta attttatttc aaaaaatata ataacactac taatatttta aatatcaata
    10561 gtgtcataaa aacttttcag attaattaaa tctttctata tattttataa taatctaaat
    10621 catgctaaat atttagacag aaaacttttt tctttaatag taaatacttt tgtttgaata
    10681 tcagaataat tatttgaata tctatctata aaaccatgat ttgcatctaa aatctcaatt
    10741 tggatatttt gtttcctaga aagttggtaa gaaatagaat caacatcaaa tgaatcgtgc
    10801 tttgcaaata ctaataaggt aggacatttt ggaattacat ccatgtaatc tctaatccga
    10861 gagccataac aacaaattat gccatcacaa aaagaatctt cactgcatct ccatgcaata
    10921 gttgctccca cgctataccc agcaacaaat acatatttat actctgattt taaatcattt
    10981 ataagctttt caactttttt ataaatatca aaaccaacat tatttataaa aaaatcatat
    11041 gcctcttgga cttttgaata tggaaaaact cttttttctc ccagaagttc aggacaaaat
    11101 acatcatatc catatttata atacttttga caaacatctt ctataaagct attaatccca
    11161 tagatttcat gcaaaacaac aatagctaca tcacttttat gtatatactt tatcagatta
    11221 tccaccacct ttacaaaaca gcaaattttt tatacttagt ttaatatatt cttctttaca
    11281 tgtcaattta cttttctaaa atcaaattta tttttggatt aatcatttat ttttaaaagt
    11341 tgtgcattat tactattaaa ttattctcta ttattaaaaa tataatcgtt agaaataaaa
    11401 aataaagtac aaataattaa aaaatatctc actcataact ttaaaaatta taagtgagat
    11461 actcctaaaa tctactttat attaaattta ttttccaaaa tatctattta ataatcctaa
    11521 aaatgcttta ccatgtctag cttcatcttt acacatttca tgtactgtat catgtatagc
    11581 atctaatcct aattcttttg ctttcttagc taattttaat tttccatcag ttgcaccata
    11641 ctcagcatca actctaactc ttaagttttc ttttgtatct gcaactacaa cttctccaag
    11701 aagttctgca aatttagcag catgttcagc ttcttcaaga gctattctct tataagcttc
    11761 tcctacttct ggataacctt ctctatcagc ttgtctactc attgctaaat acattcctac
    11821 ttctgtacac tcaccaacaa agttagctct taatccttcg attatttctt catctacacc
    11881 ttgagctact cctattctgt gctcatcagc ccaatttttt tcgcctttca tttcttcaaa
    11941 tttatcagct ccaactttac atactggaca ttgtgctggt gcagcatctc cttcatgtat
    12001 atatccacat actgtacaaa caaatttctt cataattact ttcctcctaa attttatatg
    12061 tcaaattaaa ttttttattt attaagttag tctatatata cccgcaccat atacctttaa
    12121 acaatttttt taaaagtttt ttctaaatac ttattttccc tgtgtcaata atatgtttct
    12181 ataataatgt ccctttaatt ttatcaattc tttatgattt cctgattcca caattctacc
    12241 attttctaaa actattatat tatcacaatt tgatatagta cttaatctat gagctataat
    12301 tatacttgtc tttccataag ttaaattatt tattgcttct tgaattttag cttctgttgc
    12361 tatatctatg ttacttgtag cctcatccaa aataagtatt ggagcatttt ttaatactgc
    12421 tctagcaata cttattaatt gtttttgacc ttcacttaaa tcttcacccc tatccgataa
    12481 tacagtacta tatccttcac gcattttaac tataaattta tgtgcatttg ctattttact
    12541 agcatatata acttcttcat cagtagcctc cagatttcca taccttatat tttccataac
    12601 tgtatcattg aatatataac cttcttgtgg aactactcca aaacaagttc tcaaatagtt
    12661 tctgttaata gagtatatat ccttaccatc taaaaatata cttccttcat ctatatgata
    12721 aaaatttgta attagattta taatagttgt ttttccactt cccgtctctc ctatgatggc
    12781 atttgatgtg ccagatttga tttttaggtt tatattatct aaaattttct tttttgaatc
    12841 ataagaaaaa cttacatctt taagctcaat ttctcccttt acaaaatgag agtttaaatt
    12901 accatcttta ttatcgttaa tctcactttt aaacatgtta tgcacttctt gctcttcttg
    12961 ttcatctata atttcaaaaa ctctttcaca tccagctatg gctgaaagta atgtattaaa
    13021 tagatttgct aattcattta aaggtcttgt gaattgtctt acataactta aaaaactagc
    13081 tataacccct atggttataa tatctttaga tgcaagaatg cctcctataa tacatatagt
    13141 tgcaaatcct atattactaa tcacattcat aataggcatt aaaagagaag tgtaaatttg
    13201 cgatttaagg gaaatattta gaagtctatc attgacttct ttaaaatcat ttacagattt
    13261 tctttcataa ttaaaagatt ttactacatt tatgtttgat atcatttctt caatatgtgc
    13321 atttagtttt cccaactcaa cttgttgttg cttaaaaaaa actctagttc tctttgcaat
    13381 aaaattactc acaaacatta ctatcggtat taatattaca ctgcaaaagg ttaaaattgg
    13441 gcttaataca aacatcataa taaaagtcat caatatgttt aagattcctg taattaactg
    13501 tactaatgaa tttgatattc ctgtactgat attatcgaca tcatttgtca ctctactcat
    13561 aatatctcca gtagaacttt tatcaaaaaa ggttattggt agatttttaa attttaaaaa
    13621 taaattactt ctcatttctc tgactatact ttgagataat ctagctgtac atatcccctt
    13681 agacagttta actgaaaact caattatata aaatgaaagt aaaataatta ttccttttaa
    13741 gaaaatggat tttgaataag tatcgatatc tattatgtca actacctttc caatagtata
    13801 tggtattaca gttgatataa ttgaatcaat tattactagc aaaaaaataa atctagtaat
    13861 cttcttatct tttttaaagt acataaaaat tcttttaaac gtttttttaa aatctttagg
    13921 cttttctttt ttctttaatc tactgacctt ctgatttcca gtacttaaat tcaatatact
    13981 tcctccttaa tttacatctt cacttactaa attttgcaac gcaaaaattt ttttataaat
    14041 tggagaacta accagtaaat cttcatgtgt agcaaatcca accacctcac cctcatccaa
    14101 gactaaaatc ttatcacagt caattataga actaatcttt tgtgaaatag ttattacagt
    14161 acatttctta ctttcctttt ctcttaaatt acttatatac tcttttatat tctttcttat
    14221 aattgtctct gtattaatat ccattgcact aaaacaatca tcaagaatta atatttgtgg
    14281 ttttttggct atagctctag ctatagtaat tctttgtctc tgtcctcctg ataaattttt
    14341 acccctttgg aataaaactt catcataagt attttcgagt tttgttataa attgatttgc
    14401 atttgctatt tcacatgatt ttacaacatc ctctttgcat atatctctat ttcccataac
    14461 tacattctcc aatatacttt ttccaaacac actactattt tgaaatacta cacctattct
    14521 tgcccttaaa ctttcttcag aaatactgta tatactacgt cctccaatct taatatcacc
    14581 tttatcaatt atatataatt tttgaattaa atttactaaa gaagtctttc ctgaaccagt
    14641 ttgaccaatc acaccaatat tttgaccttc ttctatttta aaggatacgt tttttaatat
    14701 tttttcatct gtatttttgt tatacgaaaa atctacattt tcaaaaatta tatcacagtt
    14761 tacaaacttt aaatcttttt ctccaacatc taaactttct tctctctcaa ttacttcttc
    14821 attctcatca accaaaacct cacttattct ctcataagat gcttttgccc taacaaaaac
    14881 attaaaaaca tgtgaaatca ttataagaga tgttaatatt ctagtcatat agtttacata
    14941 agcaactata atcccaattt taatttctcc tgaataaatt aaatgccttc caatcagtaa
    15001 tattattatt atggaaacat taatgattat tgtaattgtc ggtctaaaaa atgacatcat
    15061 agttgtagct tttctactat ttttaaaaag ttcatcattt aactcttcaa agctttggtt
    15121 ctcataatcg tatctacaaa atagtttaac tacttttatt ccacttaaaa actgtcttaa
    15181 ttttgaattt actttatctg tactttcttg tacattttta aataatctat accctatctt
    15241 catattaagc aatattataa taaatataat taaaactgca attagaataa ttattgacat
    15301 ctttaaattt aattttatag ctataattaa acttcctata cacattattg gtgactttat
    15361 aaaaactctc atcattccat taacgaattg ttgcatttga ttgatatcat tagtaattct
    15421 tgttattaat gaagctctac caaatttatc tatttgtttt aatgagtatg tatttatctt
    15481 ttcaaataca tcctgtctta aatcataaga aaaatgctga gaaactcttg ccgagattac
    15541 acttcttatt attgcaaata ttgcacctaa aaaagttata cctatcatta tagctcctgt
    15601 tgatagtatg tagtctaagt tcttatttga aacccctata tctattattt ttgccattat
    15661 agttggttga gctaaatcac aaaaagcttc caatgttaaa aaaaatactg ctgttagaaa
    15721 taatactata tattttttta tgtatttttt aaaatacctc attcaatcat cccttcatag
    15781 aattttctca ttaccaaaag tatatcataa tggctaattt aattgattat atcaattata
    15841 aatatttgat ttattattaa tttttatacc atatatttta taaattattt gtttttattt
    15901 cctatattta tatatattta tagcatacta ttatatataa tatatatgta ttaaaattaa
    15961 gacaaaattt taactttact atttgtgtaa aaagtaatat aatttaaaat tgattataaa
    16021 tctaactttt ttctttatca aaatatttta ggaggtgaga catttgaaaa aaactaatag
    16081 tttaaaagaa attttaaact ggaataatat caagaattta agaccttcaa agggtttaaa
    16141 aaatatatta gaaattttta aaagtgacct aaaagatata cgtggcaatt atgccgtact
    16201 tgtaatagtt ttaggtcttg cttttttacc atctctgtat gcttggttca atataaaggc
    16261 atcttgggac ccttatggaa gcactgctaa tatgcttgta gcagtaacaa atgaagataa
    16321 aggaaatgaa atagcaggac aaaagattaa tgtaggagac caattaattg aacaactcaa
    16381 agataacaag agtttaggtt ggcagtttgt agatgaaaaa acggctcttg aaggtgttaa
    16441 aaaaggtact tattatgcat cacttaagat tccaaaaaat tttacaaaag atttgacatc
    16501 tttaattaca gatgatgtta aaaaagggga aattatctat actgttaatg aaaaaataaa
    16561 tgcagttgca ccaaagatta cagataaagg tgcttctaca atacaactac aggttaatca
    16621 gactatagta aaaacagtta gtgaaatcgt tcttggtata tcaaacggag ttggtgcagg
    16681 aattgaacac agtcttccaa aattgacaaa agttgaaaat tcccttattg accttcaaaa
    16741 gagatttgac catataaata agactgttga tttagcttct gatgctactt caaaattatc
    16801 tgatacagtt aaagatatta aatctgattt acctacaatt aaaaaaactc tgaatgatac
    16861 aaaactgtta tcaagtgact taaaaaaatt cttagaggat acaaatgata atttagacga
    16921 actttctcct cttattaagt ctgacttaaa tttaatggtt gacctttcat cttctgcttc
    16981 ttctcttaca ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg tacctaagtt
    17041 gatagataat ttatcagaaa aactttctaa tttgcaatct ttaaataata ctttggtgga
    17101 ttttttaact aaattaaacc aacttacatc aaataataga ttggatgatg tcatagataa
    17161 tcttgaagac tctagcaata aaatagattc atctatatca acattaaatg atattaaaaa
    17221 caaagttata tctggacaac aaccatctat aagtgctttg aataatgttt taagtctatc
    17281 aaatggtatt ggaagaatta atttaaatat tttaaataat tttgactcaa aaatatcaaa
    17341 acctataaat agtatatttg caaacagtat aaaagtagca aatgatataa ttactgtttt
    17401 agataaggca gaagcaaaac ttccaaaggt tgaagaaata cttactactt ctctaaaact
    17461 ttcaggaaat gcacaggaaa gtatatcatt gataagagaa agacttcctt tagcaaaagg
    17521 aatgttggat gatttgattg atactttaag caaaattagt aatggtgaag atatgaaaaa
    17581 attagtcagc ttgcttgaaa gtgatatatt gacaaagtct aatttcttaa aagaacctgt
    17641 tgaggtgaaa acagagaaat tatatccaat tgaaaattat ggttctgcaa tgacaccatt
    17701 ttatagtgtt ctttctacat gggttggtat attacttatt tcagcactat tgtctacatc
    17761 tgtacatgga aactataaac cttatgaaat ttactttgga agaggcctta cttttttaag
    17821 tatagctctt gtccaaggtt ttataatagg aactggagat atattattgt taggagtaac
    17881 agcaaaacat ccaattttat tgatagttct gcttatgttt actagtatgg tatttaactt
    17941 tatagtgtat tctttagttt ctgtatttgg aaatgtagga aaagcaattg caatagtact
    18001 attagtcgtt cagattggtt cttctggtgg tacattccct atacaagtta cacctacatt
    18061 cttccaaaga ataaacccta taatgccatt tacctatgca ataggtgcta caagggaagc
    18121 agttggtggt atatatgctc caaatctaac taaagatata ctagttctat tactatttat
    18181 gattctgtct atttgtttaa atgtacttct aaaaggacct ataaatagag ttataaaacc
    18241 atttaatgat aaatttggcg atagtaattt aactggtcat taaaaaatta attctgtctt
    18301 taaatatatt tttttaataa tattctaagt tcatattaaa aggtagtagc taaaaagcta
    18361 ctaccttttt ccaacttaaa aactaatatt tactacaaaa actgtcatag aaaaaaacga
    18421 aaaaattcgt tctttcctaa atttatctta taaatattta gatatattcc tacttaataa
    18481 gcagagctat acatagtatt taatgtataa cttacattat ttaactcata taatcaatta
    18541 ttcctgaatt taaattatat aaatgctcaa aaccttcttc agctaacaaa ttacatgcag
    18601 taacacttct atgaccactt ctgcaataaa ctactacttt cttatcttta taatcgtaaa
    18661 tttcatctat attattgatt aactcttgta aagatatatt tatagcattt tctattttat
    18721 attccttaaa ttcatcttta gttcttacat ctaataataa aacatccttt tcatttttca
    18781 tcatttcttt aagttgcttc ttatttatat tcttatacat atgtctaaaa ttcctccatt
    18841 ttatttatat attacctata ccctttattc tttatacaac aacaaatatt atttttatat
    18901 tttt
//
LOCUS       DAKBNN010000062        18653 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000062 DAKBNN010000000
VERSION     DAKBNN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.062"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            302..1462
                     /locus_tag="OUB02_001816"
     CDS             302..1462
                     /locus_tag="OUB02_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HCT8885576.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1804..1965
                     /locus_tag="OUB02_001817"
     CDS             1804..1965
                     /locus_tag="OUB02_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885577.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2158..2787
                     /locus_tag="OUB02_001818"
     CDS             2158..2787
                     /locus_tag="OUB02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCT8885578.1"
                     /translation="MKIVTYNIHKCMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            3026..4285
                     /locus_tag="OUB02_001819"
     CDS             3026..4285
                     /locus_tag="OUB02_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HCT8885579.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4544..5539
                     /locus_tag="OUB02_001820"
     CDS             4544..5539
                     /locus_tag="OUB02_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HCT8885580.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5785..6852
                     /locus_tag="OUB02_001821"
     CDS             5785..6852
                     /locus_tag="OUB02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCT8885581.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7386..9296
                     /locus_tag="OUB02_001822"
     CDS             7386..9296
                     /locus_tag="OUB02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCT8885582.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9359..9982)
                     /gene="ytaF"
                     /locus_tag="OUB02_001823"
     CDS             complement(9359..9982)
                     /gene="ytaF"
                     /locus_tag="OUB02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HCT8885583.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10170..10814
                     /locus_tag="OUB02_001824"
     CDS             10170..10814
                     /locus_tag="OUB02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885584.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            11028..12086
                     /locus_tag="OUB02_001825"
     CDS             11028..12086
                     /locus_tag="OUB02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HCT8885585.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12190..13374
                     /locus_tag="OUB02_001826"
     CDS             12190..13374
                     /locus_tag="OUB02_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCT8885586.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKGDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13581..14294
                     /gene="rpsB"
                     /locus_tag="OUB02_001827"
     CDS             13581..14294
                     /gene="rpsB"
                     /locus_tag="OUB02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCT8885587.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14373..15284
                     /locus_tag="OUB02_001828"
     CDS             14373..15284
                     /locus_tag="OUB02_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCT8885588.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15369..16073
                     /locus_tag="OUB02_001829"
     CDS             15369..16073
                     /locus_tag="OUB02_001829"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCT8885589.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16099..16656
                     /gene="frr"
                     /locus_tag="OUB02_001830"
     CDS             16099..16656
                     /gene="frr"
                     /locus_tag="OUB02_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCT8885590.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16659..16862
                     /locus_tag="OUB02_001831"
     CDS             16659..16862
                     /locus_tag="OUB02_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885591.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16891..17625
                     /locus_tag="OUB02_001832"
     CDS             16891..17625
                     /locus_tag="OUB02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCT8885592.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17638..18417
                     /locus_tag="OUB02_001833"
     CDS             17638..18417
                     /locus_tag="OUB02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCT8885593.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGTDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7680 a         1858 c         3230 g         5885 t
ORIGIN      
        1 ttaataaaaa taaatataaa acaaaataaa taaataataa aatatttagt ttaattatct
       61 ttacaaataa aaattttagt aataaaataa ttgttgaaaa agattttaaa ttcagattat
      121 tcatttctaa agtaattaat gtgcaaatct ttattaatat atgtcaatag ttaggtatat
      181 atgtatttaa ttagtatgat atagataaaa gtatttattt taattgtcta ttatctgaaa
      241 aagtctttac ttagaaatca taaaaatcaa attaattagg aaaagaaagg gtgtgtataa
      301 tgtgattgag actggatttg tatattgtag tttcttggtt gtattcgtag gtgtaatttt
      361 ttttatggtt gataaattga aatggaaggt atttgatgtt attaatccaa tgcttttaat
      421 atacttaggt tcagctatac ttggatcatt tggtttattt gcacagaatg aagatgttgg
      481 aacgtatcaa aacataataa cagtgaactt ccttccatta atgttagtgt ttttgttaat
      541 tcaatgtgat ttaagaaaaa tattaaagat ggggcctaaa atgttattat catttttctg
      601 tgctacattt acatttatgc ttggatttgt tatagcattc ataattttta aaaattcaat
      661 gggtgcagat tcatggaaaa ttcttggggc agcatctggt tcatggatag gtggctcatc
      721 aaatatgatt gcagcagcta atgctctagg agttgcagaa gaaggactta gttatgcaat
      781 acttatgggt tctataggat atacagtatg gatgtctgtt tgtctaatgt ctgtgaaatt
      841 tggacctaaa tttaacaaat ggacaaagtg cgatacaagt tttatagatg aggttacatc
      901 aaaacttgaa aaagaaaata aagataatac ggcacctacg tttgtagaac ttatgatttt
      961 actttctgtt gggtttggag ttgcgtcttt tgcacaaatt gtatccaaga tacttccaac
     1021 ttcaggtata cttaatgaaa ctatgtgggt agtacttgtt gcatctacag tatcaatatt
     1081 attgggtatg acaaaaatat caaagcttaa agggagtaca ctggtaggaa atgtgtttat
     1141 gtatttactc cttgcagcta caggtagtaa agcaaatttc tttggattgt cagaagcacc
     1201 tgtttacata atgtttggat tggtagtagt ccttgtacat gctatttcat ttatagggct
     1261 tgctaaacta tttaaacttg atttatttac ttgtgaagta ggaagtatag ctaatatagg
     1321 aggagtaact acagctccaa tgattgctgg ggttcacaat cctgtattaa taccagtagg
     1381 tgtacttatg gggcttctag gaaatattat agggacttat tgtgctctta tggtaactca
     1441 aatattacat atgttagctt aataaaaata tggtataaaa ttaataattt aaataagaaa
     1501 agtcatttca atttaagcag aagcttttac tgaaatggct tttttgtttg acaagataat
     1561 ctatttcaag aagatgtttt gacatattta gtgtataata ttgtaaaatt tactaaaaac
     1621 gagatatatt taatttttat attgaatcga acgtctgtat gatgtataat aaatatacag
     1681 aacgaatgtt tgcagaacgg gtgttttttg aatttatttt aataatttat gaatatatat
     1741 cagaatgcat aaatatattt aaatataaag attaaaaaga atttaaagga gtgttgataa
     1801 cttatgataa ttccatcaag aagaatacaa gatataacat taacaaattt aaaaaatggt
     1861 gaagtaactt taattgaatt ggatgaaata tacaagaaga tgggattttt atttgttgtt
     1921 agtgaaggca aattgaaaaa gataaaaaag gagaatcatc attagaaatg aatgaaataa
     1981 taaatattat aatagtttat aaaacctaac ttatgtagaa tataaatact taagttaggt
     2041 ttttttgtga taaagtttat aagtactcaa ttttataaga taaataatgg tcttaagtga
     2101 aaagtaaata agtttaaatt ttagtaagta ttatattgca taagaggtga tattattatg
     2161 aaaatagtaa cctacaatat tcataaatgt atggattcaa ataatagatt aactctaact
     2221 aaaatggggt tatatttaaa acaattggat tgtgatgtaa tttgtttgca agaagtatta
     2281 tatcctcaat ttttagcttt aaaagctgtt ttaaatatgg atggtgtctt tgcgacaaat
     2341 gtaaaaaaag taaatatgat atatggtatt tgtacattta caacatctaa aatgttaaac
     2401 agtaatcatt ttttcttaac tagcaaaaaa gaacaacggg gagctctttg tataactata
     2461 gatgcatatg gaagaattat aaatgtaata aatactcact tgggacttga taggcaggag
     2521 agagcaaaac aattagacga aataatagac tataggaaca gattagtggg gatagttatt
     2581 ttatgtggtg attttaatga gaaaaatgtc tttttaagta tgtttaatga tatggctatt
     2641 tcactaaaca agtcttactt accaacattt gaaaaatcaa actctagaat agattacatt
     2701 tttgttaata aaaatactga actaaaagga tacacagtag aaaaaattta cctgtcagac
     2761 cattatcctg taataggtta catttaaatt tcaatataaa tgaaattagt tgttataaaa
     2821 taaacaattt tttgttaaga aatagtaaaa gaaatataat aaaaaatatc aaaaataaaa
     2881 ctaattataa gacataaatt tacaataaag ttacatttga ttaaattcct tgtttaacaa
     2941 aagctattca aatataatct aaggtagttt tgaaatggga actctattat gcaaatttaa
     3001 gctagtgtta aaatgaggtg aagttatggg cagaagaaca acaagagttt caagattagg
     3061 taaagaaaaa aaacgaagtg ttttaattgt attggcagct ttagttctaa taatgggtgg
     3121 atttacttat ttttttaaca gtaaatttct gtacaatggg aaaatcgcta aaaatgtata
     3181 tatagaagga gtaaatgtat ctgatatgac taaagcagag gcattaaaag ctataacaga
     3241 taagtatact ccagaagatt taaatcttgc ttatgatggg aaaaaatata caatatcacc
     3301 aaaagatatt gatttaaaat atgatacaga agatgttgta aaagatgctt atgaaagtac
     3361 aaaaaaaggg tcctattttc aaaacttaaa aaaatatatt gatataagag tgaataaggc
     3421 aaatatgaag ataaaagctg aatatgatga ggcaaaactt agttccaaag tgtcttctat
     3481 agcagatagt ataaatgtta agatgaaaaa tgcttctatt agtgttggaa gtggtggact
     3541 aagttataca gactctgttg taggtagaga atttgatttg gcagcaaata aagagtctat
     3601 atataatatg ataaaaaata aagagcataa aactttagaa cttaaagtaa atcttcaaaa
     3661 accagatata acaactgaac aagtaaaaac tgtaaattct gttataggtc aatattcaac
     3721 tacttattca caagctgtag agggtaggtc atataatgta ggattatcag caagaaaaac
     3781 tagtgatgta ttattaatgc caggtgaaga attttcatac aataaactta caggacctag
     3841 taataaagca aatggttaca aagatgcacc tgttatagtt tatggtaagc tagaacaatc
     3901 agcaggagga ggtgtttgtc aaacatcttc aacagtttat aatgcggctc ttctttcggg
     3961 tatggagata actcaagtta caaatcattc atctgcatct acttatgtgc ctaaagggag
     4021 agatgctact gtgagcgatg gaggattaaa cttaaagttt aaaaatccat ataagcatcc
     4081 tgtatatatt aaaaattatg caggtggagg aagtgtatca tcagtaatct atggtaattc
     4141 tggtgacaaa ccaaatatat ctatagaggt aaaacaaact ggtcagaata aatattctac
     4201 atatcgaata tttaaagatt caagtggaaa ggtcataaaa aaagaacata tatcaaatag
     4261 ttcttataaa gaattaaaaa aatagataat tttttagaga aatatttgta atataaaagg
     4321 gagtgtctca aaatgaactt tttagtttat gaggcactct ttttctgtga aatcaaatat
     4381 attttcatca tttcaacaat acaaaagaag cgtatctcta ttttgagaca gccttttttg
     4441 taaaaattaa cactattagt tcttgtttta atattacaat cattaatata tagaaaatat
     4501 tcctatatat tggtataatt gtaaatagat ttagttatat aaaatgtatg aggaggtgac
     4561 atggttgaat tttataggca atagatatga ggtagtaaat tctgacgacg ttttagaaat
     4621 taacaaaatc tataaagcta gagatgtttt ttacaggaaa aatgtgttaa taaaagttat
     4681 aaagcacaat aattatatct gtgaagattt tgtttcaaat ttaattgatg aaagtactgc
     4741 tttagatgag ataaactctc cttatatact aaaaataata gatgttggga ttcactgtac
     4801 agaagaaaca acattatatt atgttgtaag tgaagacttt aatggtatcg gactagatga
     4861 attaattcta ggtaattatc ttcatttaga agcaattgta aacataatga tacaagtttt
     4921 aaaagcttta gaaatgatac ataggaatca ttcatatcat ggtagtttaa aatcgagtag
     4981 tataatagta gatacagaat acaatataaa aatttgtgat tttggaatta caaaagcaaa
     5041 taatggggtt aatacaagaa gctatggaaa caggagatat ttatgcccac atcaactatg
     5101 tataaattat actgataaag aaagtgattt ttttgcaaca ggtcttattc tatttgaatc
     5161 tatatttaaa aaattgcctt ttggagaatc aaattcagaa gaaaaaatgc tacaatctat
     5221 tgataaggga ttagattgga atagtataaa agcaattaat ggaaatactg aacttataaa
     5281 tgttataaaa aggcttcttg gaagaaataa taagtatacc agagccaatg atattattat
     5341 tgatttaagt aaagtaatgt atgaaaaagc atatatagaa gttgaagatg agaaagaaga
     5401 agagcagaaa caagacaaag ttatacaagt taaaaaagaa tataaaagta aaatgagaaa
     5461 aaaattacat aaaaaacttg ttgttgccgg tctagctata gtcatgattt caatgattat
     5521 tattagttcc ataatataaa aattgtatat taagtatttt aaaatcaatg tctataaagg
     5581 atatacatag aaattttagt gtagtcttaa attatgaaag gaagtggtta ttatgttaac
     5641 aaaaaataat gaggtattaa acgtctagga gtattttaac aaatagccac ctgcttttac
     5701 agtttagaat aaaaaagctg tggctaaagg tcacagcttt ttgctacttt ttaaaggcag
     5761 gagaatatta aaaaggagca aattatgagc aatataaaaa taatagcaag taataaatgt
     5821 tggattgaag atatagcaag gaaacaactt gaagatattt caaagttaaa tggaatttta
     5881 agagtagtag gattacctga cttacatgca ggtaaaatac ctgtaggttt agcagttgag
     5941 actaaaaata ttatttatcc acatataatt ggaaatgata taggatgcgg aatgacttta
     6001 tttaaaactg gagttctaaa aaagaaattt aaaaaagaca aatggattaa gtcacttagt
     6061 aagattaaag atttgtctga tatagaaata aaaaacacat ataaagaaga atgtccaatt
     6121 ttaaatttag gaactatagg gagtggaaat cattttgtag aaattcaatg tataagtgaa
     6181 atttataata aagaacaatt tgaacaatta aaattttcat ctgatgatat tatgatgtta
     6241 gttcactgtg ggtcaagaaa ttatggagaa gatattttaa aaaaatttta tgataaagat
     6301 ggacttgaag ttgaaagtga gaaagctata gattatatta aaaaacatga taatgcttta
     6361 atgtgggcag agcgtaatcg tgaagctatc agtaaaaaaa ttatgcagtc aataggtaca
     6421 tctggagatg ttgaaacaat attctctata aatcataact ttgtagagaa aagggaagat
     6481 aaatttatac atagaaaagg tgctgtttca agtgaaagag gagctgtgat tataccaggt
     6541 tcaagaggca gtctatcata tatagttatg cctactgaaa atacagagat atctttatat
     6601 tctctatcac atggagcagg taggaaatgg tctcgttcag tttgtaaatc tcgccttaaa
     6661 agtaagtata gtaaagatac tataaaacaa actaaattta aaagtcagat tatatgtaat
     6721 gatgtaaatt tattatttca agaagctcca gaggcatata agaatataga gcagattatt
     6781 gaaagtttga tagaatatga acttattcaa gtagtggcaa ctatgaagcc attgattact
     6841 tataaaggat aatttttaaa gttaaaaaag attaaaatta tattataaat agctaatttt
     6901 atggaacatt taaaagtgtt ttattattag ctattttttt attttgtaga gacgataatt
     6961 tatagagcta aattctttga tatataatga ttacaaagtt agactgtatt ggaaactgca
     7021 catacatgaa ttgatgacaa tataagtgat tccaaaatat aaatgaaagt ataagcttat
     7081 tttcactata aaaattttca ctgtacaaaa acattaatga cattattttt attgacataa
     7141 atatataatt tatggaataa tattagaagc actgatataa gtgtttaaat aaggatagaa
     7201 aaggagaagt gttttatgaa aaaaaatact aacattaatg agtccggagt taaacttctt
     7261 aataaataag gagtttgata tataataact ccttatttat taagttgttc gcaaaataac
     7321 tattatcaaa gtaaatgata gggtgattta aaagacgata acttataaat aaaggagaaa
     7381 aaataatgaa tagtaataca tttgaaagat tacaactaaa tgaagttaaa gaattaataa
     7441 aaatacattg tgtaagttca ctaggaaaaa atttaataga taaattaact cctagtggga
     7501 atattggagt agtaagaagg aaattagcag aaaataaaga agctagaaag ataattgaaa
     7561 atagtaatca cataccactt gaaggattgt ttaatgcagg ttctacaata gataaaattg
     7621 aaaaaggaat gataatagaa cctgttgaac ttgtaaatat tgaagatttt ttaagaggtt
     7681 gtaggaagat gaaagctttt atgttagaaa aagaatttta ttcacctaca ttaagttctt
     7741 atgccttaaa tataacagaa tgtaaaagta tagaagatga gataaattac tgtataaaat
     7801 ctaataaagt tgattctaat gctagtaaag aattaaaaaa gataagacga aatatagaaa
     7861 ttacagaagg aaagataaaa gatagattaa ataaatttat aacatcaact gtaaataaaa
     7921 agtatataca agagtttata ataagtaaaa gaaatgatag atatgtaata ccaattaaat
     7981 cttcttataa aaatgaagtt aatggtacta tattagatac atcatcaaaa ggaaatactg
     8041 tatttattga acctattagt gtttcaaatt taagtacaga gttaactatg ctaaaagctg
     8101 atgaaactat tgaagaatac aaaatattat cttatttaac agaacttata tttgagaaga
     8161 taagtcagat aaaattaaat atagagatac tttcagaata cgatatggta tttgcaaaag
     8221 ctaagtatag tcaaaaaata aaaggaatta ctccaaaaat aaataataat ggctatataa
     8281 aaataattaa aggtaaacat ccacttctaa caggagatgc agtaccttta gattttgaaa
     8341 taggcaaaaa ttatagaagt cttataataa caggtcctaa tgcaggagga aaaactgtaa
     8401 cccttaagac tgttggttta ctaactttaa tggttcaatg tggatttgat atttctgcaa
     8461 aagaaggtag tgaatttagt gtatttgaaa aagtttttgt agatataggg gataatcaaa
     8521 gtattgaaaa tgccttaagt actttttcat cacatataaa aaatattgca gagattatga
     8581 gtttatcaaa taattctact cttgttctgt ttgatgaaat aggaagtgga actgaaccta
     8641 atgaaggagc tggtttagca atatcattat tagaagagtt ttacaaaatg ggatgtataa
     8701 ctatagcatc aactcattat ggagagataa aaaagtttgc tacattgcat ccagaatttg
     8761 aaaatgctgg tatgatgttt gataaagaaa cgttagaacc attgtataag ttaactattg
     8821 gaaaatctga agatagtaat gctcttttta tttctaaaaa aatgggaata aaaaatagag
     8881 ttttagatag agctaaagaa tatgtaattg atagaaacta caatttagat ttagttaaaa
     8941 gaagtaagct acaatctaat attgaagata aaaatagttc tgaagtcatt ttaaactata
     9001 cagattataa tgtaggggat aaggtaaaat tactagatga agatgatttt ggaattgtat
     9061 ataagcctat ggacaagttt aataatgtag aagtattgtt taatgataat tttatttcag
     9121 taaatataaa aagattacaa cttagcataa aagcatcaga attgtatcca gaagggtatg
     9181 atttagatgt attatttact agttttaaag aaagaaaatt agaaaaagat attcaaagag
     9241 gttcaaaaaa agctcttaaa aagattcaga aagaaattag agagaataga aaataaatat
     9301 aaaaagcaca aaagaaagca atctaaagag aaaagtttat ctttagattg ctttcttatt
     9361 aacatatctt cataaatcca atgactaaca agattaaacc acttaaccaa gataagttta
     9421 aatttacgtt tttaacaaac atatttccta gtaaacaacc aagtaatact gcaattatat
     9481 ttgatattaa agaaaataga ataacttgta tataattcac acctacaagg ccactaccaa
     9541 aaccgatagc taatccatct aaacttaaag caagtgcaag tgtaaatgct tcagcagatt
     9601 taagcacttt agatttactt gtatctaaga catcaggttc gatacttaca ttaaaattta
     9661 atttaaggtc taacatatta aaattataat tctttgagtg tatagatttt ttcttaaaat
     9721 aagatttaaa aaaactttca attagtctag cacttcctaa taaaaataaa ataaagaaac
     9781 atattaacat tgttatattt tgaggtaaaa aatctctaac tattgtacct gcaaaaagcg
     9841 atattgctaa aatagctgaa caaactacgc tgataattat atttgaatat aagggaactt
     9901 taactttatt tacaccatat ccaaagcttg tcacaaaggc atctatacaa agagaagtaa
     9961 ctaataaaat tgattcaatc acagtagatt aacatccttt ctcttaaatt ttgtacttat
    10021 attagttata gtattgtaaa aatggataaa ttgttacaga taatccaata aattgttgat
    10081 aaaattccaa catcatacaa cacctaaata acaaaagaat catatcatat aataatataa
    10141 aaccaatcat aataaagcgg gtgaaaataa tggaacaaaa taataaggtt gggaatttcg
    10201 gtgatttgtt gaagatttta ttagatgaaa aagaattatc aatgggagaa ctaagtaaaa
    10261 aaagtggaat tgataaatct actatatctc gtatagctaa taataaacaa aagccaaata
    10321 taaatcattt agaaaagatg gcaatacatt taaatattaa cctagaagaa ctgttgaaag
    10381 cttcaggata tgaatttaga aacagcaata atcaaatatt taatgcagat agtgatttta
    10441 gtaactttga tgatattctt ggatttgcaa atttaatcaa tgataagaac tttaatggaa
    10501 atattgaaaa agagttaagt aagtgtaagt tatatgtcca aacagatgaa ggtaaaaaat
    10561 tactttttga taattttaat aaaaaaatag attctattga aaaacaaggt caatttacag
    10621 atagattaag acaaatgtat gctgattttt gtacagatgg acttacaata aagaaatatt
    10681 tattgattgg aagtatgcta ctttactttg ttatatcaac agatgtgatt ccagacttcg
    10741 tgttcccaat aggatttatg gatgatttag tagcattaaa tatagttaca aaattactta
    10801 aaaatgataa gtaagtttta gaatcatatt taatatataa ataaatcaaa gtatataaaa
    10861 tttattattt tttatatgaa acaaattaaa agcacaatga agtagattaa aaatttaaaa
    10921 ctaagaatct aatatatatt ttgtaactat ttaagtatat aaaataaaat aatatccaaa
    10981 ctaatacaaa atagtaataa ttgttttgta ctggaggata tatatttatg aattgtaaat
    11041 ggatatctaa aatagatgag agaaaaaaat gtcatagaga agcagattct tctggatatt
    11101 gtatctttca taaagaaaat aagtctgatg aagagataca gttgatgatg gatactcttc
    11161 ataaagaaga gataagtgaa tttaatggat ttgtttttga aaatgaattt aatgcggaag
    11221 aaatattaac ttacaattat aaaatactgg atttttctga atccatattt aaacaaaaag
    11281 caaattttaa aaaatatatt tttaaaaaga atattatttt taactataca gaatttagag
    11341 ataaagtttt atttaatggt tgtgtattct tagaaaactg tgattttaat agaaccatat
    11401 ttagtaaaca ctatataaat gaccgtatat ttgaaaaagt gaaatttaaa ggacctgatt
    11461 tggtagttaa taaagtagaa aattttccac gaatggatgg aataattttt agcatgtgta
    11521 caaaatttgt gttaaagaat gttgaatatg gaaaaagtga gtatgagcat ggcaagataa
    11581 attatagaat agctagaaat caagcaacaa aaattggaga atatgagatg ataggatttt
    11641 actattataa ggaaagaatt tatagtagta aaataatgaa acgttctaat tatcctactt
    11701 tcagtgatta tttagttgaa aaattttttg accaaatagc acgttacaca acaggttatg
    11761 gagaaaagcc atggaatata ttattagtta ttatagcaat aataagtgtt tttgctttat
    11821 tatacttatt tgtaggcata gaaagttcaa atagcacact tgtagcttta gatataaata
    11881 atataggtga ttactcacta agtgagattt tcaaaatgta tatggattta tggtatttta
    11941 gcatggctac atttagtaca gtaggatatg gagatatggt tgctacaagt ttgattggga
    12001 aagcattggc aggtatagaa gtattttttg gagttacaat aggagcaatt tgggcatctg
    12061 tgattattaa aagaatgata aggtaatatt tgtgtaaata tggataggta ttaattacta
    12121 gatataaatt ggactaaata gggaaaattt aaatataaat atacttaata atccaagaag
    12181 gtgaaaaatt tgaaaaatgc actaaaaaaa attatatcat tagccatgat tctagttttt
    12241 ataataccaa attattcata tgcagatgat aaatctgtag acaagtattc aaaaacatct
    12301 atacttatag accaagaaac aggtagagtt ttatatagta aagaacctga tatgaaggta
    12361 ccattagcta gtttaagtaa gatgatgact tttttacttg caatagaagc tattgaaaat
    12421 aaagaagtaa aagaaactga taaagtaaaa atagacaaat ctattgcatc agtaaaaggg
    12481 tcaagttata aacttaaaga tggtgaagaa gtgccactta tagagcttat gagaggtctt
    12541 atgattgtat caggaaatga tgcggcaatt gctatagcaa aacatatttg taaaacgaaa
    12601 gaagcatttg tagatagaat gaataaaaaa gcaaaagaaa taggaatgag taatacacat
    12661 tttctaaatc caaatggtct tccaatatac gatttaagca accccaaaaa accagcaaaa
    12721 gaaaatatct ctactgcaaa agatatagct attcttggaa aatacatgtt tgaccattat
    12781 gaaaaacaag taacagctat aactgatatg gagacatata caaattcaga tagaagtttt
    12841 gaaaaaagca atacaaatgc attattaaga attataccag aagtagatgg tataaaaact
    12901 ggatatactg gcaatgctgg atattgctta tcattttcta tggttgtaaa taaaaatgat
    12961 aaaaatgaga aaaaacgtag agtaataggg gttgtattag gtactaacca taaaaataaa
    13021 agaacctctg cttcattagc tctactgaaa tatggaaaag aaaatttcaa tatgaaaaaa
    13081 gtaattgata aagatagctt tattggtaag aaatatataa aaggaatgaa agagttagag
    13141 gttacattaa aagcaaaaga tgaactttat acaatattaa aaggtgatga aagtataaaa
    13201 tcagaagtta agattaaaaa tatagagtac cctgttaaaa aaggtgatac tatgggtatt
    13261 ataaaatatt atactaattc aggtgactta cttggaagtg tagatatagt aagtaataat
    13321 agtatagata acgtatcatt aaaaacaaaa ttaaaaatga aatttgcaaa ttaaattcaa
    13381 aaaatggtat aaaatacaca aaaaaatata aaaatagaaa atatggaaaa aatattgtta
    13441 tttcttcacc tctgtgatat actactagag gtgaaaaaat aaaataaatc acacacagca
    13501 ttgggatcct taaaaggtgc ctattacatg gttttttagg gagaagatca agctggaggt
    13561 ataaaaccag gaggtaagat atgtcagtaa tatcaatgaa acaattatta gaagcaggtg
    13621 ttcactttgg tcaccaaaca agaagatgga accctaaaat ggctaagtat atattcacag
    13681 agagaaatgg aatatatata atcgacttac aaaaaacagt taaaaaagtt gaagaagctt
    13741 acaagttcac taaagaagta gctgaaacag gaaagccaat cttattcgta ggaactaaaa
    13801 aacaagctca agatgcgata aaagatgaag ctgaaagatg tggaatgtac tttgttaatg
    13861 aaagatggtt aggtggaatg ttaacaaacc acaaaactat aaaaacaaga ataaataaat
    13921 taagagaatt agaaaaaatg gaagaagaag gcgtatttaa tgttcttcct aaaaaagaag
    13981 ttataaaatt aagagctgaa aaagaaaaac tagaaaaata cttaggtgga ataaaagaca
    14041 tgcctgaatt accaggtgca atgttcgtag ttgacccaag aaaagaaaac atagctattc
    14101 aagaagctca tagattaggt ataccagtag ttggtatagt tgatactaac tgtgacccag
    14161 aacaattaga ctttgctata ccaggaaatg atgacgctat aagagccgta aaattaataa
    14221 ctggtgctat ggcaacagct gtaattgaag gtagacaagg tgctgaggaa gaagtagctg
    14281 aagaccaaga ataataattt gacaattaaa tggtaagggt cgcccttacc atttataata
    14341 tggaatagaa taagtaggag gaaacttaaa aaatggctaa tataactgct caaatggtaa
    14401 aagagttaag agaaagtaca ggcgcaggaa tgatggactg caagaaagct ttacaagaag
    14461 ctgaaggaaa catggaaaaa gcagtagatt tattaagaga aaaaggatta tcaaaagctg
    14521 ctaaaaaagc tggtagagtt gctgctgaag gtctagttgc aatagaaatg aatgatgata
    14581 atacagttgc ttctatggta gaagttaact cagagacaga tttcgttgct aaaaacgaag
    14641 acttcaaagt gttcgttaaa gatgctgctt gtatggcatt agctactgat aaagaagata
    14701 tagcttcttt attaggtgaa actcataaag agggaataac tttacaagaa gttttaaata
    14761 atagagttgc aaaaataggt gagaaattag actttagaag atttgctaaa gttgtaacta
    14821 atggacaagt tgctggatac attcacggtg gtggaaaaat aggtgttctt gttgaaatgg
    14881 aaacagaagc tagagatgct aaagttttag aattaggtaa agatgtagct atgcaagttg
    14941 ctgctatgaa tcctaagtat gtttcaagag atgaagtaga tgctgaatac atagcacatg
    15001 aaacagaagt attaactcaa caagctttaa atgaaggaaa accagctaat atagttgaaa
    15061 aaatggttaa aggaagatta gaaaaagaat taaaagaggt ttgtttatta gagcaaactt
    15121 tcgttaaaaa tccagatata actgttaaac aattagttgc tgatgttgct aaggcagtag
    15181 gttctgatat aaaagttgtt aaagttgtga gattcgaagt tggtgaaggt atacaaaaga
    15241 gagaagaaaa ctttgcagaa gaagttgcta agcaacttaa gtaataacca aagagagaac
    15301 acgcaggtgt tctctttttt aaaaaataga aaaaattgga tatgaaggat tgaaatagga
    15361 ggtttctgat ggataaacca atgtacaaaa gggtattgtt aaaattaagt ggagaagcat
    15421 tagcaggaga aagaggtttt ggtataaata atgatgtagt taatgatatt gctattgcta
    15481 taaaaaaaat acaagaaata ggtgttgaag tagctgtagt agtaggtgga ggaaacttct
    15541 ggagaggcag aaccagtgaa ggaatggaca gaactactgc tgactatata ggaatgttag
    15601 ctacagttat gaatgcaatg gcgctacagg atgcactaga aaatatagat gttgctacaa
    15661 gagttcaaac tgctatagac atgagacaaa ttgcagaacc atatataaga agaagagcag
    15721 ttagacactt agaaaaggaa agagttgtta tatttggtgc aggaacagga aacccttact
    15781 ttacaacaga tacaactgct gctttacgtg ctgctgagat ggaagcagaa gttatattgt
    15841 tagctaaaaa tgttgatgca gtatatgaca aggatccaaa agtacatgca gatgctaaga
    15901 aatttacaga attaagttac atggaagtta tacagaaaga attaaaagta atggattcta
    15961 cagctacatc tttatgtatg gataataaaa ttccaataaa ggtatttgag cttacaacag
    16021 agaatataat aagagcagta aaaggcgaaa atataggaac tactgtaaaa taatgaagaa
    16081 tgaaggagga gtaaaattat gaaactggaa atacataagc aattagaaga gaaaatgaat
    16141 gggacaattg atgctttaaa gtttgaattt ggaactataa gagcaggaag agctaatgca
    16201 caaatgctag acaaaataag agttgattat tatggaactc caactccaat aaatcaaata
    16261 ggtgctatat ctgtaccaga accaagaata ttaatgatat ctccttggga taaatctgct
    16321 atgcatgaaa tagaaaaagc aatagctaac tcagacttag gattaaatcc atcaaatgat
    16381 ggtgaggtta taagactttc tgtacctgct ttaacagaag aaagaagaaa agaattagct
    16441 aaaaaagcta gtaaagctgc agaagaattt aaagttagaa taagaaatga aagaagagac
    16501 gccaatgaga agattaaaaa gatggaaaaa ggcggagaat taactgaaga tgaattaaaa
    16561 aaagcacaag atgaagttca aaaaatgact gataaattta taaaagaaat agatacatta
    16621 ttatctaaaa aagaaaagga tataatggag gtataatatt gaaaaaatat tataaccttt
    16681 taggtcttca tgtagatgag gtaaaacaat attttgatga gcaaaatata aattataccg
    16741 taaaatctat tgaaggtaaa aaggataaag aaaaacttgt agttcctaga gttataaaaa
    16801 tatctgagat aggtgatagt gtagaactga taatcacata cttttctgac tccttagttt
    16861 aaggagtcag aacaatattg gaggcaaaat atgaataaca acattatata tgatatagac
    16921 ttaaataaca tacctactca tattgctatt ataatggatg gaaatggaag atgggcaaaa
    16981 gctagatttc ttccaagaac tgcaggacat aaggctggag tagaaactat aagagatata
    17041 gtaaaagaat gctcaaaatt aggtgttaag cacttaacat tatatgcatt ttcaacagaa
    17101 aactggaaaa gaccgaaact tgaagttgac acacttatga acttattatc tacatactta
    17161 agaaatgaaa tagctgaact tcaccaaaac aatgtaaaag taactgctat aggagatata
    17221 agtgctttac caaaaacttg tataagagaa ttaaactcag ctaaggaact gacaaaaaac
    17281 aatacaggag ttaacttaaa tttagcatta aactacggaa gtagagcaga tataaaaaat
    17341 gctcttatag atatagtaaa aaattgtgaa agtggtaaaa tagatataaa caatattgat
    17401 gaagatatta taaaaaacta tttaagcaca aagtctatac cagatccaga cttggtaata
    17461 agaacaagtg gtgaacaaag attgagtaac tttttacttt gggaagttgc ttattctgag
    17521 ttttacttta ctgatataca ttggcctgat tttaaaaaag aagaactaca gaaggctata
    17581 tacgtatatc aaagaaggga tagaagattt ggaggactta agtaaggaga ttattttatg
    17641 cttacaagaa ttattgcttc actagctctg gttccattat ttctatttgt tgtatatgga
    17701 ggtattcctc tatatatagc tgagacagca atagtttata ttgcattgca tgagttttat
    17761 aaggcattta aaataaaaga tgtacatcca atattcatta taggatattt attttctatt
    17821 tatttagctg taaaaaatat ttttaattta ccattagaat atacttatgc agtaattttt
    17881 attttatttt tagcaagtat aatttatatg ttgatgggca aaaacaatgt tatagatgtt
    17941 tcgattacgt ttttaggagt tttttatata ggagtatttt tagattttat aattataact
    18001 ataaatggat ttgaaaaagg tagtatatat gtatggctta tatttgtgat atcttttatg
    18061 acagatatat ttgcatattt tagtggttat ttacttggaa aacataagct aattcctaaa
    18121 gttagtccta aaaagactat agaaggagct attggaggaa taataggaag tacactttgt
    18181 tgtattttat ttggatattt atttggcact gatttgcttc aattagcgat aataggaagt
    18241 ataggaagtg taatagctca attaggagat ttatttgcat cttctataaa gagatatgta
    18301 gggataaagg attatggaaa gataatacct ggtcatgggg gtatattaga tagatttgat
    18361 agtgttatat tagttgcacc ttttgtatat agtgcgatta aattttttat tagataaaga
    18421 ttggttggat ttaaaagagc aatctataat tgctagtaat gaaaatagct atgtaacttt
    18481 taaatgcaga gatattctgc agttattagt tacatggcta ttttcattgt tcaaaaattt
    18541 agaagacctt ggggaattgt ataagaatag gtattacttt ataatagtat actatatttg
    18601 tagtgagtat attttttata ctataactat cattaaaaaa tatttttttg ttt
//
LOCUS       DAKBNN010000063        18651 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000063 DAKBNN010000000
VERSION     DAKBNN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.063"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            92..796
                     /locus_tag="OUB02_001834"
     CDS             92..796
                     /locus_tag="OUB02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT8885594.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            793..1563
                     /locus_tag="OUB02_001835"
     CDS             793..1563
                     /locus_tag="OUB02_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCT8885595.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            1560..2339
                     /locus_tag="OUB02_001836"
     CDS             1560..2339
                     /locus_tag="OUB02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HCT8885596.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            2391..3083
                     /locus_tag="OUB02_001837"
     CDS             2391..3083
                     /locus_tag="OUB02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885597.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            3102..4526
                     /locus_tag="OUB02_001838"
     CDS             3102..4526
                     /locus_tag="OUB02_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885598.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNNINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(4766..7234)
                     /locus_tag="OUB02_001839"
     CDS             complement(4766..7234)
                     /locus_tag="OUB02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885599.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKITKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIGLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(7237..7905)
                     /locus_tag="OUB02_001840"
     CDS             complement(7237..7905)
                     /locus_tag="OUB02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885600.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(8191..8718)
                     /locus_tag="OUB02_001841"
                     /pseudo
     CDS             complement(8191..8718)
                     /locus_tag="OUB02_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            complement(8935..10095)
                     /locus_tag="OUB02_001842"
     CDS             complement(8935..10095)
                     /locus_tag="OUB02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8885601.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(10083..10757)
                     /locus_tag="OUB02_001843"
     CDS             complement(10083..10757)
                     /locus_tag="OUB02_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885602.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            complement(11579..12406)
                     /locus_tag="OUB02_001844"
     CDS             complement(11579..12406)
                     /locus_tag="OUB02_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCT8885603.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            12788..13108
                     /gene="sugE"
                     /locus_tag="OUB02_001845"
     CDS             12788..13108
                     /gene="sugE"
                     /locus_tag="OUB02_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCT8885604.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(13241..13921)
                     /locus_tag="OUB02_001846"
     CDS             complement(13241..13921)
                     /locus_tag="OUB02_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCT8885605.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            14317..15369
                     /locus_tag="OUB02_001847"
     CDS             14317..15369
                     /locus_tag="OUB02_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT8885606.1"
                     /translation="MKSVRFYGIRDLRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGIWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            15416..15832
                     /locus_tag="OUB02_001848"
     CDS             15416..15832
                     /locus_tag="OUB02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8885607.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            15861..16334
                     /locus_tag="OUB02_001849"
     CDS             15861..16334
                     /locus_tag="OUB02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885608.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIIIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            16381..17139
                     /locus_tag="OUB02_001850"
     CDS             16381..17139
                     /locus_tag="OUB02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8885609.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            17163..18002
                     /locus_tag="OUB02_001851"
     CDS             17163..18002
                     /locus_tag="OUB02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT8885610.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKDELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
BASE COUNT         6505 a         2441 c         3107 g         6598 t
ORIGIN      
        1 aaaattagta ggtgttatag aattgttatt ttttcatgtt agtattctct tagtaacaga
       61 tttaaggtat tacaagaagg gagaaattaa tatgaaagat ttaattcttg aaacaaaaaa
      121 cttatcaaaa agatatggag aacaaatggc agttaacaat gtttctctac aaattgaacg
      181 aaatagtata tatggacttt taggaccaaa tggagcagga aagtccacaa cacttaaaat
      241 gcttgttggt cttcttcgcc caacaaatgg tcgtattatt tttgatagaa agccttggaa
      301 acgagaatct cttgcaaaaa taggttcttt aatagaatcc cctgcattgt atggaaacct
      361 tacagcagag gaaaatttgt tagtacatac aaaaatattg ggaataccta aagataaaat
      421 ttatgaagtg ttggaaatag taaatttgaa aaatacaggt aaaaagagag cttcacaatt
      481 ttctatgggt atgaaacaaa ggttaggaat agctattgca cttttgagtg acccagagtt
      541 gttaatacta gatgaaccaa ccaatggtct tgaccccttt ggcattcaag aattgcgtga
      601 attgattgct tcttttccca aaaaaggtat aactgtaatt ctttctagcc atattctatc
      661 agaggttgct caagttgtag ataatatagg aattataaat ggaggacgac tattatttca
      721 aggaatgcct gaccctaatg aaaatctaga agaatttttt acagatgtta ttcttaaagg
      781 aggacaaaac agatgaatgc tttacaatca gaacttttaa agtataaaag aacatttatg
      841 ggtaaactta ttgttttttt cccagtgtct tttgcagcat atgcatttat aatgcaatca
      901 acactcatgc aaaatccatt atctcaaaca acttcttggg cgtggcaaaa tcttttagca
      961 ttaattttta actggtggtc atttttattt ctgcctattg gatttgcatt gttcgctact
     1021 ttggtagctt tccaggaaaa aaaagctgga aattatcgtg ctttacgtac acacaatgtg
     1081 tctcctatga cactttggat taataaagtt atagctatgg cagtctacag ttttatttca
     1141 acgctagttt taatagtcgt aacaattata actggtttaa tattgaaagc tggtccagtt
     1201 ccttttggac agattatagg ggcaagtatt gtatgctggg ttgtttctct tgctatactt
     1261 ccacttcagc tatggctagc tacatggaaa ggtatgttcc taagtatggg agttggagct
     1321 ttaggtatga tatttggtgt tcttgctgcg acaaaaccat tttggattgc agtaccttgg
     1381 agttgggcag ttagaatggt atgcccaatc atcaacatac atcctaacgg tactatatta
     1441 gaagctggag acccactttt gaatacttca gtgattccta ttgggattgt tgtttctttg
     1501 gttgtgttta tagtattaac agcacttact gcagcttggt tcaataggag ggatgacaaa
     1561 tgataaaact attatcatca gaatggcttc gagcaaagag gacagctgtc cagtggctta
     1621 ctctttgtat gcctattatt ttttcattat gtgtggtttt ttatctcaca actaaatctg
     1681 gaagtacaca agaatttgct tttgaaggat tttttacagt atggactgta ttcatcattc
     1741 ctattggagt aggtatttta gcaggattta tagtgcagga ggaagagctt gctggaaatt
     1801 ttaatggttt tttatgtgta ggtatttctc gtgtcagatt atatctggga aaatttcttt
     1861 ttcttctgtt ttgcctaact atatgcacat ttattgctac gttgatactt tgcataggaa
     1921 tgagtattgc agtgccatct ggagcaacaa tatggttatt cctgtcagca gcaggacttg
     1981 tggtgattgg gacacttcca ctattagcta ttcatctttg ggtgagtttt gcatggggta
     2041 tgggtgcatc tattggtatt agtattggtg gaattttgat ggcagcaata cttggattaa
     2101 ctagcgttgg tgaaaaagta tgggcatttg ttccatggac atggccagta aaactaggta
     2161 tgcttccagg aatttatttt attaaagaag ctggaactat ttcaactgag gcattttact
     2221 ctaaagtgat gcaaacagct agtatagagc ttatagtagt agcaataggt ttgattatat
     2281 tcttgatagg aggcgttatt tggtttaaaa tgtgggaggg aaggaaaagt tatgagtaga
     2341 cgaatattgt tttaatactg accttcccat aagagaaagg tggtgttata ttgagtaaaa
     2401 tattgattat tgatgatgaa atggacctag taatgctact tgaggatgaa ttgaaggcaa
     2461 aaggacatga agtgttgata gcttatgatg gtcaggcagg tatagagtta tcaaaacagg
     2521 agcctgacct tattatactg gatattatga tgcctaaaat gaatggattt gaggtatgtc
     2581 aggcaattag agatgatgtt ttatgcccaa ttatattttt aagtgcaaag caatcagaaa
     2641 ccgacaaaat caaagggttg acctttggag gggatgatta tgtaacaaag ccatttggac
     2701 tgcgtgaact tatggcaagg attgaagcta atctgcgtag ggaaaagcgt tcacagtata
     2761 tcaatgaaga gaacaagaga tctactttgt attttgggaa gttatgctta tatatgaagg
     2821 aacgtgcaat taaaattgat ggtaagaata ttgatttaac aaaaatagag tatgatattg
     2881 ttgaattact agcactccat gcaggacaag ttttctcaag agaacaaatc tatgaaaaag
     2941 tatgggggtg tgattctgaa ggtgattcct caactgttgt tgaacgagta aaaaaaatac
     3001 gagctaagtt ttctgctgtg acaccagaaa aagaatatat ttccacagtt tggggtattg
     3061 ggtataagtg gaataaaatg taaagagaga gaggttatta aatgaaaaat caatccctta
     3121 ttacccaatt tagacatact tttattttta ttataatagc cagtatagtt gccacagtga
     3181 ttacttatgt atttgctctc tatttgtata ttcattcctt aaataaggat atctatccac
     3241 caaactatta tgagcgacaa gttcctagaa ttgaaaaata catcaatgaa aaaaacatag
     3301 ctttgttatc acaatcgaat gaagaaggtt taaaaagaac gattcgtggg gatgatatgc
     3361 tatatcaagt tgtggataat aatggcaata ttttatatgg tactaacccc aaaaaactat
     3421 ttaaaacaaa agaagaatta tttaataata atatcaataa aactgttcgt aaaggtggtt
     3481 atatccatac tgttccaatc aaaggggata atggaaagat tgagggtgct gttattctat
     3541 tctaccaagt gaaaatcacc tttgcaaata ttagagggag atttgtattt gcagttatta
     3601 taatggctct gttttctcca tttctatata tcgtgggttt tactagatgg ctttctaagc
     3661 gctttgtaaa gaatatcaat caaccattac atctattgat tgatgcgtcc aagaagataa
     3721 aagaaaagga ccttgatttt gaaatcgact attattcaga taatgaactc ggtaaacttt
     3781 gtagtgcctt ttctgaaatg aaagacgaat taaaaggctc tttatctgca cagtggaaga
     3841 tggagcagga acgtgttgaa atggtggaag ctctagcaca tgatttaaaa tctcctcttt
     3901 ctattatttt gggatataca gatgcactta ttggtaataa tacagatgac aatgaaaaac
     3961 ttcataggta tcttacagtg ataagagaaa atacagaaaa aagtgctgct cttgtccaaa
     4021 agatgcaata tacgtctgac ttagaaaagt caaatataca attaaatctt gttcctataa
     4081 atttacctga atttttgagg caaaaggtgc aagactatga attacaggct catcaaaagg
     4141 aagttgaact tatattgaaa atgcagggga atatacaatc cccaattcaa atcgacgtgg
     4201 ataggttgac acgaattttt gataatatca tatccaacag tctgcaatac actccatcag
     4261 gtggtaatat tagtattact gtaaaggatg aaaaaaattg tatttcttat gagatttgtg
     4321 attctggtag agggtttagc tctaaagatt taaaaaaggc tcttgataaa ttttatcgag
     4381 gagatgaagc taggcagaca aaaggtggac attctggtct tgggctttac attgtcaaac
     4441 aattagtgga acagcttgga ggttctgtta aaatagaaaa ttcaaaatca ggtggagcat
     4501 gtgtgaaatt ttggcatagc atttaactat atattttaaa ttaaagggga gtgtctcaaa
     4561 gcgaaatttt taattcgtga ggcactcttt ttttgtgaag ttaaatatat tttcattatt
     4621 tcgacaatga aaaaaggctt atctctattt tgagacaggc cttttttaaa gcttatatta
     4681 tagttaaaaa gatttattag ttagatttga aagaaaatag agacagtcat tgataattta
     4741 atttatatta ttatctgtaa aatagttata aattgatatc tatacagtca attatattca
     4801 tctttttaac ttttcttaac ggaataagag tagctataat acatgcaagt acagaaataa
     4861 tagtagcttg taaaattgaa gtaattggta tattagtgaa atcaagcttt tctatagttg
     4921 ccatgtttat aataatagca ctggcatacc cagcaattga accaaatata gcagcaaaaa
     4981 ttccataata aacaccttcc cataaaaaca ttttatataa gctactatta ctcataccaa
     5041 tagctctttt aactccaatc tcattagtcc tagtatttat atttgtatga gttgtgttaa
     5101 tgatgtttaa agtaccaatc aatcccacaa ataatataac tgcccatgct agaaattcta
     5161 tttgtttaaa actctcttta agttgtttat ctgattcaat atatgatatc caatgagagc
     5221 caggattatt ttcacaaatc tgttcgatag acaatcctat ttgttctaat tttgatttat
     5281 ctttagcata aatatttatc tggttgaatt tgtttttatt tgtcaaggtg ttataaacag
     5341 aatcatatac aataacatca acaccattta tccagccatt accttgtaaa aagaacattt
     5401 tatctactgt actatatata tctagttgtt ttttgttgat tgtgatacta tctttaggtt
     5461 ttaaatttgt atgtctagtt tttacacctg gtgtagaaat agcaatcgga ttttttataa
     5521 aacagataga accattttta aagtcttcta gtatagaatc tgtaatagaa ggcattaagt
     5581 cttttaaagt ttgctcatca attccaatga tttttaacat ctcaccacta ttttcaaaat
     5641 ttatatctgt atctatttcc ccctgtttgt attcagagta ctttataaag gaagtgcgat
     5701 tgacgttttt cattttagtt attttatcta gtgttgattt atcaaaacca gatatttcat
     5761 ttgttaagga aaaatctcct tcttttaaat tacttactga cctagataca tctaatacat
     5821 tagagaactc actcagtgca acgaatactg ttatactcat aaataatgat agtatagtta
     5881 ttatagttct tcctttattt ctttttaagt ttagccatgc tagatgtctt tcaaagtttt
     5941 ttattgtttt ttctctttta atttttcttt tgattttggt ggtactacca accatagcaa
     6001 ttttgggaga gacatgtgaa gcatatattg ctgatggtaa tgctgaaata aatgaaaaaa
     6061 tcaaagatac attagtgctt aatactaatg ggaacatata tgctgttgta tttttatgta
     6121 taagctcagt aatttcttta ttatcatttg ctagaagtat atctggattt aagatattag
     6181 tgaccattcc agttatgcct ttagaagata taattccaat tacagctcca ataggtatgg
     6241 caacagtaca taaaattaag atttgtaaaa taactatctt atatatctgg tttggttcag
     6301 ctcctattgc tcttaaacat ccatactctt taattttctt tgtaacagag atttttaaaa
     6361 tattatagat tacaagtcca gaggccagta aaaccaataa ggcaacaaat aaagatacca
     6421 atataatcat agatatacca tcactattag aactattttc atctttttca aactgtatct
     6481 taagagcatt taagtaaatc caattatatt gaattgaatt gtgagaaaga tttatttttt
     6541 tagctaattg atttactatt tcttgaaact tttcttgctg ttttatttta aaatccaaag
     6601 aggataaaat atatctctta ggtaatagat tttctgatgt acctttacct acaataccat
     6661 ttactatacc acttacatat cctgtataat catcctctaa aatgccactc aatttaaagt
     6721 tagctgtgta attatatggt ggtatggtat catttaaaag tgaaatatct aaattcatag
     6781 gtatagtcac tcctagtctt ggttttatac ccataagctt tagtgtgttt tcatctaagg
     6841 ctatttcgtt tttatcttta ggcaaatgtc cagattttaa tttcattaat ttggggtagt
     6901 tagaaagtcc tgttttatca tactctttta caagtacaga gataccagaa tcctttattt
     6961 tactactgcc aatagttatt gaagtaccag cttgataaac tctatcatct ttctttatgt
     7021 catcaacttg atttttagtg agttggtgaa aggatacatg tctatcacca ttaaaagaac
     7081 gggcttgttc aattcttaaa ttttgtagga taccaattga ctgtccaacc acagttgtca
     7141 taatagttga tagtactatt gctataatga tgaatgtagt cataagtttc tggcttttta
     7201 attcttttat tgaaagtgaa aggtaattct tcatttctat ttcacctcca taagaacacc
     7261 atcaataatt gaaatttttc tatcagcaag ttttgcaatt ctatcatcat gagtaattac
     7321 cactaaagtc tttccaaact gttttgaagt ttttataaga agattcataa cttcactgcc
     7381 acttttactg tccaagtttc ctgtaggctc atcagcaaat attatatctg gttttgcaat
     7441 aagtgctcta gcaattgcaa ctctttgttg ctgaccgcct gaaagttcat ggggaagatg
     7501 attgagtcta tctgcaattc caagtaatga tgaaatttca tttaaatagc ctttatcagg
     7561 ttgtttttta tctagaagta caggcattga aatattttca gatgctgtaa gaacaggtaa
     7621 aagattaaat tgttgaaaga cgaatccaaa ggattttctt ctaagtgcag atagttcttt
     7681 ttcagtgtgt ttatatatat ctttatcatc aagtaacact tgtccagagg taggattatc
     7741 taatccactt aaaatatgta aaagtgtgct ttttccactt ccagaaggtc caattacaga
     7801 aacaaattca cctgagttta tttctaaatt tacattatta agtgcgataa ctttattgtt
     7861 attagaacca tatatttttg ataattgttt tgcttttata atcataaaaa acactctcct
     7921 tttctatata agaagagtgt aagttttaaa actcaatttt ttcccaactt ttagcttaaa
     7981 tctaaaaagt cttcactatt tgcggataat tcgcagtgct gataagcagt ttcagtatca
     8041 tttttaattt aaaatgtaat ttatgcacta aaggctttac aatacaactc tattgcttta
     8101 aaactatttt acacataaac ttgtggataa ttttttatgt tttacttcct cattattata
     8161 taaattattt ttgtcacaaa ttctaagttg tcacataaat atacttttta tatcacgatt
     8221 taattctttt gattttatca taattgctat ttcttcaaaa gaagaaagaa aagccacgat
     8281 acatatcata attcctgcaa catcaagacc aaagcaaata tagagtaatg gaaacgcaaa
     8341 gatagaaatt ccagtgattt tattcatcaa agtatgaagc gatgaaaatg tgcgatactt
     8401 aaaaaaacca atactgtaac taatcaaccg taacaatgct attgcaacaa tacatcacca
     8461 tagatacatg ggaagagcaa tattttttcc gacagtaacc aaaattgtaa ccgcaaaaat
     8521 gaaatcagca atactatcta actttgcacc tattccactt tgttgtctca actttcgggc
     8581 aataagacca tcaagtatat cactaattcc acaccaaaaa taacatatcc agaaaggcag
     8641 tgaacagaat gtagaactaa gcattagcaa tgcaaacaca atacgagaga atgtaatatt
     8701 attagcaaaa cttttaatat aatcacctcc aaaattctaa tttcatacta ttctacatat
     8761 ttaaatatat atttctatta ttgtttcaaa tgccttttgc caaaatatta taattgaagc
     8821 aactcttaca acttctacaa aatagtatat atattggttg taaattgaaa tacattttat
     8881 aaattctaaa ctaacaaatt ttatataata gcagtgaaag cttacaaaat cattttaatt
     8941 tcttgttgag aatttagcag taattttagc actgttagta gaattatttt caatagaaat
     9001 agagccacca tgtttttcac ataatatttt tgagatataa agacccatac caaagtgtgc
     9061 attgctgtca tttaaagtct tatctctata gaaaggtctt aaagctaatt ttagagattc
     9121 cttactaaat cctataccat catcttcaat tacaaaggta aggagttctt gttcaagata
     9181 tataagtatg gatacattat ttctagcata tctaaaagca ttcgatatta tattttcgac
     9241 gactctaaga atgatatttt catcaaaaaa tagatttata ttatttattt gatttttaag
     9301 tgtaaaagat tttccttctt gtttagcaat gatagatata ttttcatcta agttttctat
     9361 aaacttgttt atattcgaca tagattttga aacaactaaa tcttctaatc gttgagcatt
     9421 actcatattt tctacatagt attcaattct gttaatatgc tcagacataa gttgtgtggt
     9481 tgataaaatc ttctcatcac taagcttacc tgttggtatg tattttgtta aatagtttga
     9541 gtaacctttt agaactgtta ggggatttct taaatcatga gaaaaagcag cattaagttg
     9601 ttttctatca ttaatcatag tccacatctt tatgttgttt ttatttagtt cagacctcat
     9661 tttttcaaaa gcattacata gattacctaa ttcatcatta ttattatatt ctaagcaaaa
     9721 gtctaaattg ttgttggata tttccattgc acctttattg agtatttcta tgggtgtttt
     9781 taattttacc ttgtaaaaaa ttatatcagc taataataaa agtcctataa aaaatataag
     9841 tggaagtaca agctggagta tgttgattat attaagtaat gttttatcat aatcactaaa
     9901 ttggttcaca ttgttttgaa ctatggttat aatacctgta tttataggtg tttttaaata
     9961 cttatcttgt atagaattat atagattatt aagtaaaatg tatgcgaaag tagtaagtac
    10021 tgatgctata attagtgaag ataaagtaag aacaaagaaa gcttttttta atgacatatt
    10081 atttatttta tccatttata accaactccc cagactgttt ctatataatt ttcaagagta
    10141 tgttttgata gtttgattcg tatttttcta atgtgttcca ttataacatc actatttcca
    10201 ttgctattgt atccccagac tctatcatat attttttctt tatcaaatac ttgtccagga
    10261 ttcatagaga gaagctctac tatatcaaat tccttttttg aaagtgaaat ctgattatta
    10321 tatatacaaa ttaacctttt ttcgtaatct attgttaaat cattataaaa tttaatttta
    10381 gtttgatttg acttcctttg ttcacgtctt aaatgggcag atactctagc tccaagttcg
    10441 tctaaatcaa aaggttttac aatataatca tcagcaccag cacgaaaacc atttatcttg
    10501 tcagtgtttt caattctagc tgtaagaaat agaatagggc atgatacata atctctaatt
    10561 ctattacaaa cttcaattcc atctatttca ggcatgttta tatcaagtag aatgatatct
    10621 ggatttttgg atattttttt taatgcgtct tttccacttt gagcagtgta aacctcataa
    10681 ccattcattt caaagtaatc ttttaagagt gttattatac cttcctcatc atctacgacg
    10741 agtattttat tgttcataag aatgctccct tctaaattaa gtttgtatag tatgtatatt
    10801 ttagtataaa aatctcaata aattatcaaa tttttagtta tatttcaaaa cttaatcatt
    10861 aaagttagta tatttttata tattaaataa atcaaggatg aaattattag aaattttaat
    10921 agaatatata tttgatattt aatacttatt ggtatgcaaa aatatagctg atgaaatctt
    10981 ataatttaag ttgaaaatgg ataaatagaa aggaaggatt ttaattaaat aaggtagatt
    11041 aattaaaaag tatataaatt ataaaaatga attaatctac attaattagt tgatatatca
    11101 attattattg tttttttcag ttgcttaaaa tatacttgat atatcaacta atgttgtttt
    11161 ttaattaaaa gaaattttag gaaagataat atcaactagg agagataata tcaacattga
    11221 agaaggtaga gcaagatttc tacataattg tgaacattat ctggcatgtg tgcattggtg
    11281 ttttaagcaa gtaattcaat agaaagatgt gactaaaaat agaaagaaat accataatcc
    11341 aaatgtaaag ctagaagaca taataagtaa aaaagtaaag atatttaata aaagaaataa
    11401 ctaatagtga ttaaattagt aaatgctgat aattagcata atcattagta attattagta
    11461 ttatttaatt aaattaattt tagcattatt tttatgaaat taattttaaa tcaaattaat
    11521 gagaacccca taaatttttt taacacttaa tttacgattt atggggttat gttctgtttt
    11581 atttcaaagt attaagttca tataaatcag ttcttctgtg cttaagaagt ggtaactgtt
    11641 gtcttatgtc aggaattaaa tctctatcaa tgtcagcata aataatacat tctttttcat
    11701 cagcttgagc aattattctt ccccaagggt ctgaaattct tgaatttcca aaagctatat
    11761 aagaagcatt catatttcta gcaggtgcag caccaacata gaatatctga ttatctaaag
    11821 cacgcattct tatagataac tcccaatggg cgggaccagt agtcatatta aatgcagcag
    11881 gtaagataac gatttcagca cctttaagag ccattaaacg tgataactca gggaaacgaa
    11941 tatcatagca aatagctata ccaacttttc catattcggt atctattaca gtgacatcat
    12001 ttccagctgt aagagtatct gattctttaa aactaacttt tcctgtaact tcaatgtcaa
    12061 ataagtgcat tttacgatgt tttccaatta gagctccatt tttatcaaat acatagcaag
    12121 tattatagat ttttccatct tctaactcag gaatactacc agaaactaga taaattccat
    12181 gtttctcagc taatttactt aatatcgttg ttgtttcacc tggatattct tcagcaaatt
    12241 taggaaaata tttattttca tatgggcagt taaacatttc tggcaaaact acaattttag
    12301 ctccattgat ggcagcttct gttaccatat ctacagcatg attgatattt tcaattttat
    12361 tttctgtagt aatcatctga catactgcaa ttttatagaa attcatagaa attactcctt
    12421 tcaatatttt tatgttatta taacatatta aataaaaatt agatattctt agattgacct
    12481 aaatgggtat ggagagaagt gaataagaat attaaaaggt atataaaagt tatttttatt
    12541 atagatggaa cccatatatt gacttttttt atatatgttg atagtattat tatagataaa
    12601 atagaataat taatagagtt ttatagggtt ccgcaattat taatgattgg tctggtccga
    12661 gataaaaccc acaacatagt ttttgttgtg aacacggaag gacaaaaacc tgggagatat
    12721 tttataatat ttctcaggtt attttattgg aaaaataaga gacatatttg aaaagaggga
    12781 tgaatttatg aaatggttat ttttgtttgt tgcaggtttg tttgaagtgt tttgggcttt
    12841 tcaactaaaa aattcacatg gattttctaa attaactcca agtatactta ctgttgttgg
    12901 aatgattgta agtttttatt ttttgtcatt agcactaaaa catcttccac ttggaacttc
    12961 atatgcaata tggactggaa ttggtaccgt cggtacagct attgtaggca tggctatttt
    13021 aaatgagcct attagtatat caagaattgc atgtataggt tttattgtgg tgggaattgt
    13081 agggttaaag ctattatcta ctaattagag tatattaatt taaataagct atgaaattaa
    13141 aggcgagaaa atgtttaaaa ataatcaaca tttaaaaagt ctttacttca tagcttatct
    13201 tatttataag tttattatat ttgtaaatta tatgtatata ctatttatct aataaaagag
    13261 cagttaaatc catagggtca actattacac catctttata gacacgcata tttccagatg
    13321 ctatttcatc tattaagatt acttcatcat tgtagaatcc aaattcaaat ttaatatcat
    13381 aaagctcaag acctttttca gctatagtat cacgaactat gttagaaatt ttttgagtca
    13441 tgcttttcat tgaatcaaat tgagcttgag tcataatgtt taaagcttct aaaccttcag
    13501 atgttataag agggtcacaa cggtcatcat cttttaaagt tgcttctaca taacaatcta
    13561 gtttagcacc ttcttcaaca catgaaccat aacgacgtaa gaaactacct acagctctat
    13621 aacgacaaat aacttctaat ccattaccaa aaacttttgc tggtagaact tccatagttg
    13681 cttcttctaa attagcactt acataatgag ttttaattcc agcattgttt agaatttcaa
    13741 agaacttaac agatgtctct aaattagctc taccaattcc atctatactt agacctacac
    13801 tattttcgcc tgggtcaaat actccatctt taccagttac atcgtcttta aatttaagta
    13861 agtaatttcc attatctaat tcaaaaacat ctttagtctt tccttgatac acttttttca
    13921 taaacaacct ccaaaaagtt aaattattat attgaataag gcattagtgt caaaaaagtt
    13981 ctgatattcg catgaaaatt tatattgata ccttatattt aatattatat cacaaataga
    14041 agggaaattt ccgaacaatt ttataaaaat aacaaaaaaa gtacatttta taaaacttat
    14101 aataagagaa ctaatataaa atgttagtgt cgtttatatt gacacacata agttttaata
    14161 taagaaactt ttgacacact ttgacacaca tttaatgata attttgtaaa atgcatgaat
    14221 acaatgctta catatattgg catgaaagtt gctttagata tttaacatat aaacaatagg
    14281 aatatattct caaataaaaa gtaggggtgg ttaatagtga agtcagttag attctatggt
    14341 ataagagact taagagtgga ggatgtggat gtaccaaaaa tattagaaaa agatgatgta
    14401 ataattaaag taaaagtagc tggaatatgt ggttctgata tatcaaaata ttcaaagaca
    14461 ggtccacata tggttggaga aattttaggg catgaatttt ctggagaagt ggcacaggtt
    14521 ggtaaagaag taaggagttt taaaatagga gatagagttg ctgtttgtcc agctatgcca
    14581 tgttttgaat gtgatgaatg taaaaaaggc ttgtattcaa gatgtaataa tgtagcaata
    14641 ataggaaata aagaacttgg aggttgtttt gcagagtata caaaggtgaa agagagaaat
    14701 ttaataaaaa taccagatga gataagttat gagactgccg ctgcactaga accagtatgt
    14761 atagcaggac atggtctttt taggtcagaa gctaaagttg gagatactgt tgtagtactt
    14821 ggtacaggtc caataggatt attttcaata caatgggcaa aaatatttgg ttctacaaaa
    14881 gtaatagctg tggatgtatt tgatgagaag ttagacttgg caaaagagtt aggggcagat
    14941 atttgtataa atgcaaaaga aaagaatatt gtagaagaaa taaaaaggtt aactgatgga
    15001 gatggagcag atattgttat tgaatcggct ggtactccac tgacatgtgg tcaagtactg
    15061 ttgctagcta aaaagggagg tacagtattg tatgcaggtg taccatatgg ggatgtagct
    15121 ttgactagag agcaatttga aaagatagta agaagtgaat taactgtaaa gggaatttgg
    15181 tttggaaact cctttccatt cccaggaaaa gaatggagtg ctggattgta tcatatgcaa
    15241 aagggtgata tgaatgttga aaaattagta acacatagaa taaatttaga agaagtacca
    15301 gcttattttg aaaaagtata caaaagggat attttctttg gtaagataat gattaatata
    15361 gataattaga ttagagtact aaataataat ccatataaaa aaggttaaga aaggtatgat
    15421 tcatatggca ggaataatta tagccacaca tggaaatatg gctaaagcaa tgttagaaag
    15481 tgctgaatta atagtaggca aacaggaaaa tgtagaaact ttaggattaa accatggtga
    15541 cagcatagat gagtttagta gaaagttaga agatggaatt gagaaatata aggatgaagg
    15601 ggttttagta ttgcttgatt tttatggcgg aactccattt aatacatctg ctattataat
    15661 aaataaattt gctaaggtat gtgagttaga atgtttgaca ggtgtaaatt tacctatgct
    15721 tttagagtta tttttaaaca gagatactat gaagttaaaa gacttgaaga atttatgtga
    15781 ggaagttggg atatcaggaa tcaaagatgt taaaactgtt ttaaagttgt aaaataaatt
    15841 aaaaatctgg aggaataatt atgagtaata tagcattaac aagaattgat gatagattaa
    15901 tacatgggca agttattact gcttggtgta agattacatc agcaaaaagg ataataatcg
    15961 tggatgattt agtagtaaag gacccattta tagttcaagt tttacaaatg gcagctccat
    16021 caactgttaa ggttgaagta catgatgtag aatcaggtgc tgaagtctta aaatcacata
    16081 atggagatga aaatttaata gtattggtca aataccctaa aactgtctta ggtttagtaa
    16141 atagcggggt agatttaaag gaattgaatg ttggaggtat gggagcaggg cttggtagaa
    16201 aaagttttta taaaaatata tctgtgtcag atgaagaaaa agaaatattt agagagctaa
    16261 tatcaaaaga tgttaaatgc tttatacaaa tagtaccaga tgcaaagaaa attgatgtag
    16321 gaacattatt ataaagaaga ctatcttgta aatctttaaa agaaaatagg gaggaagaat
    16381 atgcacattt cactaattca agcagtatta atagcaattt tttattatct ttcttggagt
    16441 ccgtggttga cttatgttgg attttttaca tggaatagac ctctattggc tggatttgta
    16501 acgggaatta tattaggaga ccctgttcaa ggagcaatta taggagcagg tataaatatg
    16561 atatatctag gctttatatc agcaggggga gctcagatgg gggacccagc ttttgcagga
    16621 tatgtaggta ctgctttagc tatagcatct aagctagatg taagtactgc aatggcaata
    16681 gctgtaccat tgggaactgt tgcaactgta ctttggattg gaaagatgac agtaaattct
    16741 ttttttgcac attgggctga tagagaggtt caaaaaggga atgttgatag agtagcattt
    16801 ataaatatag taccaccaca gatactgtta tttgcaatga gttttatacc agcattatta
    16861 gttgtttatt ttggtccaaa tgcgatagat ggtatgttgg aagtaatgaa tgataatgtg
    16921 ttacatgtat ttaatgtaat tggggctatg ttgccagcac ttgggattgc tatgaattta
    16981 aaattgattg gaaacaagtt tactatgcct tttttcatat taggaatact aatggctgtt
    17041 tattttaaag tagatataat agtaatatct gttattggag taatacttgc tcttacaatt
    17101 acttcaatta agtacggaaa agatagtgct acatcttaaa tacatgtgag ggggaaaata
    17161 ttatgtcaga tataaaaaat gaagtatcta agaaaacgtt aagcaagaaa gatgtaataa
    17221 aatcatggtt aagatggttt ttctttgcac aatctaatta caattatgaa agattgcaat
    17281 caacagcttt ttcacattca atgttgccag tacttaaaaa attatatcca gataaggatg
    17341 agttaaaaca agaagtggag actcatctag cattttttaa cacagaacct atatgtggat
    17401 gtgttataca tggaataaca atagctatgg aagaagaaaa agctaatgga gcagatgaaa
    17461 taagtggaga tggtatgaat gctatcaaaa caggcttgat gggtccacta gcaggaattg
    17521 gcgatacttt aactcaagga gttataactc ctatagtgtt ggctgtttgt attggtttaa
    17581 ctgagggagg agcatcaatt gcaggaccta tattatttgt aatagctcaa tatataataa
    17641 tgacttcaat ttcttttgga atgtggacaa atggatataa gtatgggaaa aaagctgttg
    17701 aaagtatact tcaaggtgga atagtaaata aggttataga aggggcttct atactaggta
    17761 ccttagtcat ggggggactt gtaggtaggt ttattaattt atctacacca ataagttata
    17821 ctagtggtga ttttaaattt agtcttcaaa cggatttact tgataagata tttccaggat
    17881 taattccttt ggtacttact ttattagtac tctttgcttt aaaaaaaggt ctttctccaa
    17941 taaaaataat gtttatactg atagtagtgg gagctattac aggtatttta ggactatttt
    18001 aaataaatat atattggtgt aagttaggat tctatatcta ttgattatat ttgtaggatg
    18061 ttggaaaatt tatattaaaa agtttggcaa ttagggtaat tttataatag attaaatttt
    18121 aaacttatgt aagctatatg tagtatttaa tattttaaaa agtatctcag aaagattttt
    18181 aatcagaatg aggtgctttt tcatttttat ataaaatatt aagtaatttc aaaaaaatat
    18241 aaatttattt aatttaatta aaatattata acatattagt tacatattaa aatatataat
    18301 cgagatattg ttaagacatt gtcgagatat tataatatgt aactaatgta ttataaataa
    18361 tagttgtaaa cttcctgtgc catatatttt aaatgcttat tcttaaattt ttgcattatt
    18421 tattaaaatt tattatgctt taacgctaaa ttgagtttga taattataaa acaaataatc
    18481 aaaaatagtg taaaaattaa tactttttgt taatattaat agataaagat aatctaaggt
    18541 ggtgattaaa ctgacaatag aagaatacaa aaacaaatac tttactggaa ataaaatcgt
    18601 actttctata aatagttttg ataataaaga actatactat catgaaactg a
//
LOCUS       DAKBNN010000064        18638 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000064 DAKBNN010000000
VERSION     DAKBNN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.064"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            17..721
                     /locus_tag="OUB02_001852"
     CDS             17..721
                     /locus_tag="OUB02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885611.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            723..2210
                     /locus_tag="OUB02_001853"
     CDS             723..2210
                     /locus_tag="OUB02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885612.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2415..4028
                     /locus_tag="OUB02_001854"
     CDS             2415..4028
                     /locus_tag="OUB02_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8885613.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4391..5221)
                     /locus_tag="OUB02_001855"
     CDS             complement(4391..5221)
                     /locus_tag="OUB02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8885614.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5294..7021
                     /locus_tag="OUB02_001856"
     CDS             5294..7021
                     /locus_tag="OUB02_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885615.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7058..8920
                     /locus_tag="OUB02_001857"
     CDS             7058..8920
                     /locus_tag="OUB02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885616.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9253..9942
                     /locus_tag="OUB02_001858"
     CDS             9253..9942
                     /locus_tag="OUB02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HCT8885617.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDDNMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10047..10733
                     /locus_tag="OUB02_001859"
     CDS             10047..10733
                     /locus_tag="OUB02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HCT8885618.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            10750..12657
                     /locus_tag="OUB02_001860"
     CDS             10750..12657
                     /locus_tag="OUB02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HCT8885619.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13110..13799
                     /locus_tag="OUB02_001861"
     CDS             13110..13799
                     /locus_tag="OUB02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885620.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14235..14774
                     /locus_tag="OUB02_001862"
     CDS             14235..14774
                     /locus_tag="OUB02_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885621.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            14793..15836
                     /locus_tag="OUB02_001863"
     CDS             14793..15836
                     /locus_tag="OUB02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885622.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            15838..16674
                     /locus_tag="OUB02_001864"
     CDS             15838..16674
                     /locus_tag="OUB02_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885623.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            16779..17453
                     /locus_tag="OUB02_001865"
     CDS             16779..17453
                     /locus_tag="OUB02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885624.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17453..18505
                     /locus_tag="OUB02_001866"
     CDS             17453..18505
                     /locus_tag="OUB02_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8885625.1"
                     /translation="MIKFKKAISLIAVGMMIVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
BASE COUNT         7206 a         1911 c         3305 g         6216 t
ORIGIN      
        1 attaaggagt gagcttatga gtaagttaat ttatatagca gatgatgaag ataatataag
       61 aaatttggta aagacattct taaaaaatga aggtcatgat gttatggatt ttaaaacggg
      121 tgatgaactt ttagaacaat ttaatataaa ggagtgtgac ttagtaatac tagatataat
      181 gatgcctggc tcaagtggtt ttgaagtatg tacaaaactt cgtgagaaaa gtacagtccc
      241 aataatcatg cttactgcaa gagatacaga tatagattat attacaggga taaccttagg
      301 tagtgatgat tattttacaa aaccatttag cccaatgtct ttggttatga gagtaaaatc
      361 tatatttaga aggatagagt ttgaaaagaa acaaaattat gataaatata gtaactcgat
      421 agatatggaa ttaaaatttg gtgatgtaat aataaataaa aagaataaaa tcgttacaag
      481 taaaaatgta aatatagacc ttacaccaaa tgaatataac ctattgactt atttgtttga
      541 aaatatagat agagcagttt ccagagatga attattaaac aaaatatggg gatacgatat
      601 agaagttgag acaagggctg ctgatgatac agtgaagaga ttacgtaaaa aaattttaaa
      661 tacaaatatt ttaatagaaa ctgtatgggg gtttggattt aggttgaagg agaaaagtta
      721 agatgaagaa tggaaatgac aagaatagaa ttggaaagat taaatccaaa tttaaattaa
      781 agttctcaaa gaaaaatata agcataaaaa ctaggatttt gttgagtata ctttgtttga
      841 taattatagt atttatgata atattagtat cttttaatgt atttcttgat acatatataa
      901 aaacaactgc gaatgaagaa ttgacaaagt ctacagaaat agtagaacat atggatagta
      961 attttagacc tattaagcca ccacaagatg atggaaagct tcctcccaaa gtattatcta
     1021 attttatgag aaatgtacaa gataaagtta ggatggcaga aacacaatca gatgctgatg
     1081 ctatggtagt tgattctaag tataacctga tatttccaac aagagaggat gatttcttaa
     1141 aaaatgttga tgagatggag ctgattaggg agtgtattca aaatgaaaaa ttagatttaa
     1201 attcagatgc aaatacacgg atatctacca gaaataagga ttattatatt tcaactgtaa
     1261 aaattactag tatatatggt gaacaagaca agtatcttat actttttata gacatatcaa
     1321 aaactctaaa tctagcacaa aagataaata ttgtattaat atctgtaatg tgttttgctg
     1381 gaatattagc aatatttaca gctgtgattt tatctgagaa aattgctaaa ccgataaaag
     1441 agttgtgtga gtttgcaaaa aaaatgggtc aaggagattt caaaagaacc tactttgact
     1501 tttcagataa agaattagta gagctctcaa ctgttatgaa taagagtgct gagtatctgg
     1561 ataagtatga taatgaacaa aaaatatttt ttcaaaatgc atctcatgaa ttaaggacac
     1621 cacttatgtc tattaaaggt tatgcagaag ctataaaata taatgttata gatagtaaac
     1681 atgcaagtga tataattctt gaagaaagtg ataggttaag tgatatggtt gaagatttat
     1741 tatacatttc taaaatagat aatataacta aagattttga acttgtagaa tgtgatttaa
     1801 gagaagtact ttcaaattgt ggagctaggc aaaatgtaag agctataaat aaggggataa
     1861 aatttattta tgaatttgat gaagaaatgg ttttatttga atgtgatgaa aaaaacattt
     1921 ccaaagcatt tatgaatctt atagaaaatg ctttgagata cgctaagagt gaaataaaaa
     1981 tagtgtgtaa gtataatcaa aaaaatatag ttgtgataat agaagatgat ggaattggaa
     2041 tcaagaaaga agatttacct catgtttttg aaagattcta taagggagta gggggaaatc
     2101 atggaattgg attatcaata gttaaatcta ttgtaaataa gcatggtggg cgtatatacg
     2161 tagaaaatgg caaaaaagga gctaaattta ctatattatt taaattgtaa aaaaattaga
     2221 gaaaatatag agatgtttga agttgttttt tatatatatc tgttgtatac tgttgaaaaa
     2281 agaacaatat taagctgaca tatattacga tagttgacat ttaaagtaaa tgcttaaata
     2341 tagatacgaa aggaaaagta aaatctgaag tgtatttagg tttaagtaag cttcttattt
     2401 tataaaaaat atgtatgaaa gaacagctga tttttaatat tacaaattta gtaacggttg
     2461 tatttgaagc ttttgtcatc catatgtttt tatcagactt tttaggccag aaagaagggt
     2521 atataaaagt tgtaagatat gctaaactgg gttttataat atgtttagga ttctgtaatt
     2581 taataacttt aaaccctaaa atcacaatgc ctttaatttt catccttatt ttttcaacat
     2641 catttttata taaaggaaat ttaaagacac gattgtttac tactgtttta ttaagtatat
     2701 tctttatact gtcagaaatt gtagttacaa gtatttttgt tttatttgta aaagaaggct
     2761 ttgaaataat gcttgaaaat aatagtataa gagtattggc gacaatttta tctaaaattg
     2821 tctttttgtt gacttgtaaa attatttgtc tatttaaaaa agatgtacat ttagatatgc
     2881 ccataaagta ttggctccct ttatttttaa tacctatatt tagtttattt ctgtctgttt
     2941 ctatctttga tgtaagtaaa ttttttagtc ttgaaagttt aaaatttcta agcttaatat
     3001 ctagtgttgg tattttatat attaatttta ttgtctttta tttatttaaa tttattatag
     3061 ataaaactaa actatctatg aaatatgaac tacttgaaaa agaaataatt cataaggaag
     3121 aattaagact tagtaatgag tgttataaaa ttattgttga acagactgat tctgtcgttt
     3181 tcgaatggaa tatcaaagaa aataaatcat ttgtatcgca ggcttggact gaaaaatttg
     3241 gatataacaa tgcatgtaag aatattttta aagaaattaa agataaagac ttagttcatt
     3301 ctgaagataa agctatattt gaaggttttc ttgaaagtat taaaaaaaag aatatgcaca
     3361 atcaagcagt atatcgttta aaaaaatcta atggtgaata tatttggtgt agaacatcaa
     3421 ttacaagtat ttataatgat gaaaatgaac ttttaagagt tgtcggagta attgtagatg
     3481 tagatagtga tataaaaaaa tatgaagagt taagaactag agcagaaagt gactcattga
     3541 ccaatatata caataaaggt acatttgaaa aattagtgga agaaactata gttatgaata
     3601 caggtgataa aaaagatgca ttattcatta ttgatttgga tgattttaaa gaaataaatg
     3661 ataattttgg tcatccattt ggtgattttg tactaaaaac atttgctgat aagatacaaa
     3721 ctagttttgg tagtaaagac ttagtcggaa gaattggtgg agatgaattt gttgtatata
     3781 tgcaagatta tgttacagaa gtaaatttgc ataaaaaagc taaagaatta aatagagtac
     3841 tttcggataa ttacactgat ttgagtttta gttttgatgc ttctgtcagt ataggtattg
     3901 caaggtatcc tcaagatgga acttcatttt ttgaattgtt taaaaatgca gatagagcac
     3961 tttatagcat aaaagcatca ggtaaaaatt catattgttt atttgaagaa gaactatatg
     4021 tacagtgaat ttgaatttag attttatgtg agagtaagat ttatatatac ttgtggctgt
     4081 ctgatttata aaatatgtta ctttgtatat ttttatatgt ttcagtaagg ttaattaaca
     4141 tgagcaaagg tgtaagtaaa tttatattag tttaagttta cttacacttt tataaaaata
     4201 caaataatag attcatttac attcttaaat aattaaagct ctttaccttc taaacatgca
     4261 taacatatcg ttataattcc agtaaataag actaaaatgc ccattcctaa gattacaatt
     4321 tcatcatcta ttactccaaa tgtttttaaa gtaacttatt tcgctttgta tcataataat
     4381 ttgtctcctt ttacatttta acttctgctg agatttgagt tagatattca ttagaatctc
     4441 gcacacttat atcatataga agatattctt cataaacaaa atccccaata cgtaaattat
     4501 tctgattaaa aaaatttaat aacttttcat aagtcttgta aatagtttca taactccctt
     4561 tatgataagc agttacataa agtccctttg gtttgattga tacaggatat ttcttgtact
     4621 ctttattaac ttttgtaaat aaatatgaat atgattcaaa gtctttatta attatatctt
     4681 cacctttaat gattgagcca atcgtaccat aatcactcaa ttcatagttg tccataaagt
     4741 taatacattc atataaaaaa ttttcttcct catctcctaa aaactgttct ttatatgtaa
     4801 caggtgtttt gaccaagtac tcttcttcat gttcttttaa agtaatagta ttcgcatcta
     4861 tattacaagc atttttagtg aatgaaatag tagattccat tagagcttga atattattta
     4921 gtttttcaat ttcattttta atatcaatgg ttttcttttc taaaagctca attagtaaat
     4981 taggtgttct tttgtctata taagctttaa tttcttttaa aggcattttt acttccctaa
     5041 gtgaagaaat tataccaaaa atgctaagtt gatggtatga gtagtatcta tatccatttt
     5101 ctttctttaa ttctggtgag aaaagaccta tatcatcata atggaaaagt gtttttttat
     5161 tgattccaca aatctttgca aattctccag ttgtaaaata attttttgaa acttgactca
     5221 taattataat tcctccttga ctatcctgtt acagtatagt ttatcatagt attttgtaaa
     5281 ggagtgtgat tatatgaaaa aattaattca ttttttaaaa ccatatagag ttttaattgt
     5341 tgtagttttg atatttacgt ttttacaaac acttggaact ttgtatatac caacacttac
     5401 agcaaatata gtaaacaatg gtgtagtaaa gggagatata gattatattg taaaaacagg
     5461 tttaatgatg atgatagtag ctggtataac agctttatca gctgtgttag tttgtaaagt
     5521 atcagcaaat ctttcatctg gattttgtag agatattaga gaagcagtat ttataaaatc
     5581 acaagattta tcaataaatg attttaataa tataggtaca gcatcaatga ttactaggag
     5641 tactagtgat attactttaa taggtcagtc tgtgtttatg tttattcaat tagtattacc
     5701 agcaccaatt ataactgttt ctggtctatt cttagcatat tctatagata aagcaatgac
     5761 aattattatt gttgtagtaa tgtttttatt catgctttca gcatttttag ttggtaaaaa
     5821 gcttataaag ttgtttaaaa tgatgcaaat aaaaatggac aatatgaata gggtgttacg
     5881 tgaggtagta actggagtaa gagttataag agcttttaat aggagtcgtt ttgaaaaaaa
     5941 gagatttgat agaactgcaa tagattattc tgaaacagca atttcaataa ataaaatatt
     6001 tgcagtactt atgcctattg taatgttaat aatgaattta ggtatagtaa gtattatttg
     6061 gtttggtggg atgagagtca gtaatggaaa tatggaaata ggtcatataa tggcacttgt
     6121 tgaatattgt atcttaattt tattctattt aatcatggga gtaatggtat ttatgtacat
     6181 acctagagca ggtgcatgtg cagatagagt aaatcagata ttagatatag aacctgaaat
     6241 tgtagatgga aatggtcata aagatactgt ctcagagaga ggacatttag tatttaaaaa
     6301 tgtaaccttt tcatatgcac aatcagaaga acctgtgtta aataatataa catttgaagc
     6361 aaaatctgga gaagtaactg caattatagg aagcactggc tcaggcaaat ctactattgc
     6421 aaatataata ccacgttttt ttgaaattca atctggagaa atatctataa atggtcagga
     6481 tattaagaag attcctcaaa aagaacttag agataagatt ggatttgtac cacaaaaagc
     6541 ttttttattt agtggaacaa tagaagaaaa tataaggtat ggaaaagaag atgcaagtat
     6601 tgaagaagta aagcatgctg caagtatagc tcaagctgat gaatttattt ctgatatgga
     6661 agacaagtat gactcatttg tagcacaagg tggaaataat ttgtcaggtg gtcaaaaaca
     6721 gagaatatcc attgcaagag cgttagttag aaagcctgaa gtatatgtct ttgatgatag
     6781 tttttctgca ttagatttta aaacagataa aagacttaga aaagcgttga aaaatgaaat
     6841 caaagattca tcagctataa ttatagcaca acgtataagt acaatcatgg atgctaatca
     6901 gattatagtt ttaaatgatg gaaaaattgt aggtattgga aaacataaag atttacttga
     6961 aaattgtgaa gtttataagc aaattgcgga ttcacaactt agtaaagaag aacttgcata
     7021 agatgaagaa ttaatctgat atttggaggt gtatgatatg agtgaaaaaa gaatagatga
     7081 acaagatata gaagtactta gttttgaaga tggcatgtca gcagaaaaag ctaaggatgc
     7141 aaaaaaaact actaagagat tattaaaata tatggcaaaa caaaagttta aaatattaat
     7201 tatttttatt tcagtattga taagttcagc gcttacagtt ttagcaccta tggttatggg
     7261 aaaagctatt gaccaactat ttaatggaat aaaaactgca gtccaaacag gtacaaaatt
     7321 cagtgtgaat tttagtacta tggggggtat agtatcaata cttctagggt tgtacttaat
     7381 tagtgcagta tttatatata ttcaacagta tattatgtca ggagttgcac aaaatctagt
     7441 tcttagcatg agaaaagatt taagtgataa actcaataaa cttcctctta agtactatga
     7501 ttctcataaa aagggggaaa cacttagtat agtaacaaat gatttagaaa aagtagcaga
     7561 tagtttacaa gaaggattaa tgcaacttat tacagcagta gttacagtag ttggttctat
     7621 tgtaatgatg ataagtataa gtgtaccact tacaatagta tcagctataa cattacttgt
     7681 ttcattgggt attactgttg taattgcaag aaaatcacaa gaaagattct ctgaaaatca
     7741 aaaggcacta ggagagttaa atagcaatat agaagagata tttactggtc aaatagtggt
     7801 taaagcattt agtaaagaaa aggatactat aagaaacttt aaaatattaa atcaaaatct
     7861 ttataatgca agtagaaaag cacagttttc gtcatatgca atatctccaa ttattagatt
     7921 tataaaccta attggctatg ttattatagc agttgtaggt gggatatttg catcaactgg
     7981 agcaatgact cttggtagta tacaagcgtt tattcaatat gtaaatcaag catcagagcc
     8041 tactacagaa atatcttata ttgtaaatat gcttcaagca gcaatagcat ctgcagagag
     8101 agtttttaca gttatggacg aggtggaaga gataaaggat gaagaatctt caaaggttat
     8161 tagcatgcct aaaggtaaag tacaatttga gcatgttaaa tttggatata gtgatgattt
     8221 tattcttatg aaagatatta atatcaatct aaatgcagga gataagattg caattgttgg
     8281 acctacagga gctggaaaga caactcttgt aaatttactt atgagatttt atgagataca
     8341 aggtggaaga attactattg atggtgttaa tataaaagat ttaaagcgtg gagaacttag
     8401 aactatgttt ggtatggttc ttcaagatac atggctgttt aatggaagta taaaagaaaa
     8461 tattgcatat agtaagtcag atgccacaat ggatgaaatt gtaagagcag caaaatcagc
     8521 aagagtagac cattttataa ggactttacc acaagggtat gacactatat tgactgagga
     8581 tgcttctaat ctatctcaag gtcaaaagca acttctaaca attgccaggg caatactttc
     8641 agacccatca gtattgattc ttgatgaagc aacatctagt gtagatacta gaacagaggt
     8701 agaaattcaa aaggcaatga ataacttgat gaagggaaga actagttttg ttattgcaca
     8761 tagattatca actattcgag atgctgattt aatattagta atgaaagaag gaactattat
     8821 tgaacaagga agccataagg aacttattga aaagaaagga ttctatgaag aactttataa
     8881 cagtcaattt acaagtgaat atgatgaaga tgttgtatga ttatcaagtt ttaggcaact
     8941 aaatgattga taaatattaa gttaaatata gtaacatgta aggttatatg gaataagagt
     9001 atttctctat ttcatataac cttttttatt tatcaaaata agataaaatt tcctttctaa
     9061 attagattga agaatgtatt tatcaatata aaagtaaaat aatcattatt attagtgaag
     9121 taaatattgt ttgccacata tatgccataa agatgcactt attttacgga gatttaacaa
     9181 agtagtgtaa agtatactct aaatataaaa taaatcaaat agagttaaat aaattgagag
     9241 gtgatataaa tagtgaataa aaaattgaac tatagatttg aaatgaagca caaaataact
     9301 gaagcagatg tgttagcttt aaaatcaaga ctctatccaa taatgaagaa agatgaaaat
     9361 gcatctaaag atggaaaata tcttattagg agtttatatt ttgatactcc tgaagataaa
     9421 gcacttttgg ataaattaaa tggcgttgca ataagagaaa agtttaggat aagattttat
     9481 aataatgact gctcttatat aaggttagag aaaaaaatca aacattacaa catgacatca
     9541 aagctatcaa ctaatttaac caaaaaagaa gtaataaata tactaaataa tgatattgaa
     9601 tttctaaaag agtcgacaaa ttgtttatta agagaatttt atttgaaatt aaaatgtgaa
     9661 agattagagg caaaaagcat agttgattac aatagagaag cttttattta tccagttgga
     9721 aatgtaagag ttacaatcga tagtgatatt aaaacttctg taaattcggt agatttattt
     9781 aataaagatt tgccaactgt ttcggtaatt gatgataata tgactgtatt ggaggttaaa
     9841 tatgatgaat ttataccaga ttttataaag gatttaattc aaattaataa gactacctct
     9901 acagcagttt caaagtatgc ttcaagtaga ttatatattt aagaataaag gctatgattt
     9961 atataaagat atacataaat aaggttaaaa aacaaataag ttaaaaataa ataaaataaa
    10021 aaataaataa gttaaaaata ggaaggatga ttttaattat gacatttaat gatattttta
    10081 aatcaagttt tatagaaaac gtatcaggtt tttcatttgt ggacagtgct ttagcactag
    10141 gttcagcttt tttagtaggt ttatttatat atatggtcta taaaaagact tatatgggaa
    10201 ttatgtattc tcgaccattc aatgtgtcgc ttgtagcact tacaatgctt acaacttttg
    10261 taatattggc agttacttca aatgtagttt tatccttagg tatggttggt gctttatcta
    10321 tagtaagatt tagaacagca attaaagacc caatggattt agtatttcta ttttggtcat
    10381 tgggttcagg tatagtttta ggagcaggtt tgattccctt agctgtcatg ggttcaataa
    10441 ttatgggatt gattctaata ttcttttcaa ataaaacaat atctgaaact ccatatatat
    10501 tgatggtaaa ttgtaataat gaagattcag aagatatagc aactgataaa attaaaaaag
    10561 tatttactaa atatcaaata aaatcaaaaa gtgttacacc agaaaaagga atagaattag
    10621 tgtttgaagt aagaatgaaa gatggagaaa caagtcttat aaatgacttg agtcaagtag
    10681 atggagttac taatgcagtt cttgtaagtt ataatggtga ctatgtagca tagagaggag
    10741 taatctttta tgaaagataa aaaatttacc cttcttatct cgattatgat tatattttta
    10801 tgtgctgtag ttggagttta tagtacatct agcaacaaaa gtgttgattt atatagtgat
    10861 gtatatattg aaaaatattt taacagagac aaggttatgg aagttaatat agagatagat
    10921 gaaagtgact tgaaggatat gaatgaaaat gctataaaag aagaatttaa ggttgcaaaa
    10981 gtaactgtag atggagatac atatggaaac gtaggtataa gaactaaagg aaattcaagt
    11041 cttatatctg tagcaaatag tgatagtgat agatacagct ataagattaa ttttgataag
    11101 tataatacta gtcaaagtat ggaagggctt actcaattaa atcttaataa ctgttactct
    11161 gacccatctt atatgagaga gtttttaaca tatagtattt gcgaggaaat gggattagcg
    11221 actccagaat ttgcatatgc taaagtctct ataaatggcg aatatcatgg tttgtatttg
    11281 gcagtagaag gattaaaaga gtcttatctt gaaaataatt ttggtaatgt aactggagac
    11341 ttatataagt cagatgaagg aagctcgttg caatataaag gagatgaccc agaaagttac
    11401 tcaaacttaa tcgttgaaag tgataaaaag acagctgatt ggtctaaaat cacaaaacta
    11461 ttaaaatctt tggatacagg tgaagatatt gaaaaatatc ttgatgtaga ttctgtcctt
    11521 aaaaatatag caataaatac agctttatta aaccttgata gctatcaagg gagttttgcc
    11581 cataactatt atttatatga gcaagatgga gtattttcta tgttaccatg ggattttaat
    11641 atgtcatttg gtggatttag tggttttggt ggaggtagtc aatctatagc aattgatgaa
    11701 cctacgacag gtaatttaga agacagacct ctcatatcct cgttattaaa aaatgagaca
    11761 tacaaaacaa aataccataa atatctggaa gagatagtaa caaaatacct agattcagac
    11821 tatttagaga atatgacaac aaaattgcat gacatgatag catcatatgt aaaagaagac
    11881 ccaacagcat tttatactta tgaagaattt gaaaaaaata taacatcttc aattgaagat
    11941 tctagtgata ataagggatt tggtaataaa gggtttgaca acaataactc taataacagt
    12001 gattctaata ataattctaa tagtgaaaat aagcgctctg gaaatcaaag tgatgaaaaa
    12061 gaagttaatg ctgaattaac atcaagcgta gtcaaagcta atacagataa tgaaactaaa
    12121 aataaaacta caaatgatag tgaaagtaag aataatacag ataaagataa aagtggaaat
    12181 gataataatc aaaagctaga aggtcctatg ggtaaaggag gtaagtcaat accaggggtt
    12241 ttggaagttg cagaagatat gagtaaaact ataaaatctc aattaagtgg agaaacttct
    12301 tcgacaaagc aaaactctgg tgatgaaagt tcaagtggaa ttaaaggtag tgaaaagttt
    12361 gatgaggata tgagtggtat gccagaacca cctgagggaa tggatggtaa aatgccacca
    12421 ggaatgggta atatggataa gggagatatg aatggtaaaa atggcaatat gaatatggat
    12481 agaaatcaag ataatccaag agaagctgga ggttttggca atagaggagg aggctctgtg
    12541 agtaaaacaa caacatactt caaattaatt ttaggtggag cttcaatgat aataatgtcg
    12601 attatgttag ttggtgtatc aagggtaaag agaagaagat ttataaagtc aaaataagat
    12661 taaaattgaa tataaataat tttaatactg ttaagtctaa atatttactt agcagtattt
    12721 ttttagaaaa taatgtattt ggttaataat ataaggtaat aaattgataa aaattaaata
    12781 cagcatatta ataatacata ataaagtaat aaaaaaactg tttaagtata ataacttata
    12841 tttagacagt ttttttcatt tataaataat ataaatgaaa ttgaaaacat ttgaaggata
    12901 gaaattggtt ggataaatgt ataaattact tcaccaaaaa ataatgacaa aaattgtaac
    12961 taagttgtaa taaaatggta gttatttagc ataaaataat gtatcataga agtatattat
    13021 aaaattaata aaataagatt tttgtaagaa aactgctggc ttttgatatt tagagtgagt
    13081 agttaaatta tgtttaggag gggtagaatt tgttgcaaaa agtattatgg aaatttttaa
    13141 agctagtagt cggtttattt atatgttcag ttggtatagt attgacgata aattgtaacc
    13201 ttggattatc tccctgggat gtatttcatc aaggactttc aaatcatata ggaataacta
    13261 taggaactgc ttctataatt gtaggttcta tagtagttat tgctgatgtc gttcttggag
    13321 aaaatgtagg atggggaact gtttttaaca tgctactaat tggatttttt atggatttac
    13381 ttttatatag taatttaatt ccagaggctg atagtttatt tgtaggaata attatgttga
    13441 tattaggatt ggttcttttg tctgttggaa tggtatttta tatgggaagt ggtttaggaa
    13501 gtggacctag agatggtttg atggttgcaa ttcaaaagaa aacaggaaaa tcacttaaat
    13561 taataagagg tactatagaa gttggagcat taatagtagg atttttatta ggtggaaaag
    13621 ttggtatagg aactataata agtgcatttg gattggggta tttcacacaa atggtatttg
    13681 gcttatttaa attagattgt agtaagatta aacatagatt tatagtagat gatataaaat
    13741 ttatcaaagt gtatatgagt ggtgagaaaa aggctgctgt tcaaaatata gttaagtaat
    13801 ttaagaataa attcaacgaa atgttaagta ttttaaatta acattttgtt tagatagata
    13861 aattgaatat taaaagaata ttaaaatatg aggattgcta gaacactata aaatatagat
    13921 ttctagcaat tttttatttg ttcaatattt ttttagtact agaagtctta ttctctattt
    13981 aatgaatgaa ttaatttata attagagata ctaaacttta tagatattaa tataaacacc
    14041 tttaaactgt gtattttcca aagcctttaa aatgcttgta tttattctaa ataaaaaaat
    14101 aaaaaaatgt caaaaaaact cttgtattat atccaaattt tatatataat tacctatgtt
    14161 gtttagaaat tttaaactca atgtttagta aacaaaatgt ttagtaaaat aaaacagaaa
    14221 ctaggaggtg caacatggat ataggtgaaa agataaaaag attaagaaca gaaaaacaac
    14281 tgacccagga agagttagct aatagatgtg aattgtcaaa gggatttatt tctcaattag
    14341 aaaataactt aacatcacca tccatagcta cccttataga tatacttgaa atactaggta
    14401 caaatttaag ggaatttttt aatgaaatag atgatgaaag aatcagtttt acaaaagaag
    14461 atatgtttga aactgaagat gaagatttaa aatataaatt aaagtggtta attccaaatt
    14521 cacaaaaaaa tgaaatggag ccaataataa taacattgta tccaggagga cagtataaag
    14581 aagaaaagcc acatgaaggt gaagaatttg gatatgtatt agcaggttca atatatgtcc
    14641 atataggcga aaagaaaaat aaagtaaaaa aaggagagag tttttacttt agaccgaaag
    14701 caaatcacta tatatcaaac gagggcaaaa ctacagcaaa agtgatttgg gtaagtacgc
    14761 caccatcatt ttagaataga ggtgtttaat aattggtaga aaatataata gaattaaggg
    14821 atatatctaa acattatgaa gaacttactg ttttagataa tttcaattta gatataaaga
    14881 aaaatgagtt tctaacatta cttggaccaa gtggatgtgg aaaaactaca acattaaaaa
    14941 taatagcagg atttgagtat gctgatgatg gtaaagtttt atttgaaggt aaggagataa
    15001 ataatttgcc accatatgaa agacaagtaa acacagtatt tcaaaaatat gctttatttc
    15061 cacacatgga tgtttatgaa aatgtagctt ttggattaaa aattaaaaag actccaaaag
    15121 atgtaataga tgctaaagta aaagaggttt tgaaacttgt tgcactagaa ggttttgaaa
    15181 gacgacaagt tgaatcattg agtggaggac aacaacaaag aattgcaata gcaagagctc
    15241 tagttaatga accaaaggta ttattactag atgaaccact tggagcactt gatttaaagt
    15301 taagacaaga gatgcaaata gaattaaaga gaatccaaaa aaaattagga ataacatttg
    15361 tttttgtaac tcatgaccaa gaagaggctc ttactatgtc agatactatt gttgtaatgt
    15421 ataagggcaa aattcaacaa atgggaacac cacaagatat atacaatgaa cctaaaaatt
    15481 catttgtagc taagtttata ggtgaaagta acatatttga tggaattatg atagaagatt
    15541 ataaagttaa tttctgtaat agagattttg agtgtgttga taaaggattc gaaaaaaacg
    15601 aaaatataga agtagtaata agaccagaag atataaaaat ggtaaagcca gaagaaggaa
    15661 tgttaaaggg gaaagttaca tctacagtgt ttaaaggagt gcattacgag atagaattga
    15721 atgaaaatgg aagattgtgg atacttcata atactaaaaa tgctgaggtt gggactgaac
    15781 taggcatgga tatatatcca gaagatatcc atataatgag aaaagagaat aactaagatg
    15841 aaaagaaaat cttttttagc atatccatat gtagtttgga gtgcgatatt tgtaataatt
    15901 cctttaatat tagtagtatt ttttagtttt acaaaagaat caggtggagg ttatgccttt
    15961 acacttgaga attataaaga agtaattgac cctatatata tgaaagtttt tgggcgttct
    16021 attcttttag caggaggagc tacattaata tgccttatag ttggttatcc agtggcatat
    16081 ataatttcaa aggctcgtgt aagtagaaga ggttcattaa tattattgtt tattcttcct
    16141 atgtggatga actttctttt aagaacatat gcatgggttg ctatattagg aaaaaatgga
    16201 cttttaaata catttttagg ttggtttgga atacaaccat tagcaatttt gtatactaat
    16261 tttgccatac ttttaggtat ggtgtataac tttttaccat ttatggttct tccaatatat
    16321 acagcactat caaagatgga taatgatttg ataaacgctg ctcatgactt gggtgcaaat
    16381 aatatgacag tttttagaaa aattatattt cctttgagtt tgccaggtgt aatgtctgga
    16441 ataactatgg tatttatgcc agcagtcact acttttgcaa tatcaagact gttaggtgga
    16501 ggtaaaataa tgctggttgg agatttgatt gaacaacagt ttacagttgt tggagattgg
    16561 aactttggtt cagctatatc gatatttatg atgatagtaa tattaatatc tatgtctata
    16621 atgtcaaaat ttggagatga atctgacaaa gaaggcggtg ggttactatt ttaaaattaa
    16681 agaaaataac ttctaatata tatttagcat tagtattctt atttttgtat gcacccatat
    16741 ttgctttggt actattttct ttcaatgatt caaaatcaat ggcaagatgg aatggattta
    16801 catggaagtg gtatggtcaa ttacttcaaa atgagagtat aatgagtgct ttatattata
    16861 ctattgtaat agctatatta gcatctgtaa tatctacaat agtaggaact ataagtgcta
    16921 taggaataca taagatgaga ggtaagagta agaaattaat actaaatgta aactatcttc
    16981 ctattttaaa tacagagata gttacagcag tagctcttat gagtctgttt gtatttgtaa
    17041 aaatggaatt tggatttaca acaatgctat tggcacacat aatgttttgt ttgccatacg
    17101 ttatactttc agttttgcca aaaatgaagc aattacctga taatatagaa gatgcagcta
    17161 tggacttggg agcaacacct atatacgctc ttagaaaagt aattttacca caaataaaac
    17221 caggtatagt ttctggattt ttaattgcat ttactatgtc aatagatgac tttataataa
    17281 gtttctttaa tgctggaaat ggtgtaagca atctttctat agaaatctat ggaatggcta
    17341 gaagaggtat aaaacctgaa ataaatgcat tatcaacaat aatgtttgca gtggttctag
    17401 gtttactttt actagctaat aaaaaagaat ctatagtaag ggggattaaa taatgattaa
    17461 gtttaaaaaa gcaatatccc ttatagctgt aggtatgatg atagttactt tatttacagg
    17521 atgtaataaa gcaactgatt ctacaaaagt tttaaatgta tataatgttg gagattatat
    17581 agatgagtct cttatagata agtttgaaaa agaaacagga atagatgttc aatattcaac
    17641 ttatgatact aatgaaatga tgtatcaaaa agtaaagagt ggtagtacac actatgattt
    17701 agtatttcct tcagactata tggtagagaa aatgaaaaat gaaggtcttt tggaaaaatt
    17761 agattttaag aatataccta atatgaaata tttagataag agttttttaa atcctgtata
    17821 cgacaaaact aatgaatata gtgttgcata tatgtgggga actcttggaa tattgtacaa
    17881 taagaaagaa gttaaagacc ctatggatag ctggaatata ctttggaatc caaagtataa
    17941 gggaaatata atgatgtttg actctgttag agatactata gggataactc ttaaaaaatt
    18001 aggatatagc atgaatagtg taaatccaaa agaaataaat gaagctaaaa atcttttaat
    18061 gaaacaaaag gatttagtat tggcttatgt aaatgatgag ggtaaggata gattacttgg
    18121 tggagaagtg gcaatgggta tgctgtattc tggagatgca gttacactaa tggagcaaaa
    18181 tcctgacctt gattatgcta tacctaaaga gggaactaat aaatgggtag atgctatgtg
    18241 tgtacctaca acagcacaaa ataagaaaga agctgagctg tttataaact ttttattaga
    18301 cccagagaat gcaaaagtaa atgctgagta cattgggtat tcaacaccta atacaggagc
    18361 actaaaatta ttagacccag aaataactca gaatcctgtt gcatatccat ctaaagaagt
    18421 acttgataaa tgtgaaactt ttgaagatat tggtgaaaat ataaaattat atgacaaggc
    18481 ttggatagag ttaaaatcaa aatagggtct tgttggttta agtaaattta cttaataaat
    18541 acttaataaa actgttcttt tttagaaaat ttattttcta attattgaac agttttattt
    18601 tttgtttaag ttatattcat atgcaattta aatagttt
//
LOCUS       DAKBNN010000065        18551 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000065 DAKBNN010000000
VERSION     DAKBNN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.065"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            169..1362
                     /locus_tag="OUB02_001867"
     CDS             169..1362
                     /locus_tag="OUB02_001867"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCT8885626.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1568..2686
                     /locus_tag="OUB02_001868"
     CDS             1568..2686
                     /locus_tag="OUB02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HCT8885627.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2817..3062
                     /locus_tag="OUB02_001869"
     CDS             2817..3062
                     /locus_tag="OUB02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HCT8885628.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3049..3795
                     /locus_tag="OUB02_001870"
     CDS             3049..3795
                     /locus_tag="OUB02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT8885629.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLAKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4078..4953
                     /gene="hslO"
                     /locus_tag="OUB02_001871"
     CDS             4078..4953
                     /gene="hslO"
                     /locus_tag="OUB02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCT8885630.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5344..6144
                     /locus_tag="OUB02_001872"
     CDS             5344..6144
                     /locus_tag="OUB02_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCT8885631.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGIHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6671..8911
                     /locus_tag="OUB02_001873"
     CDS             6671..8911
                     /locus_tag="OUB02_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCT8885632.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEAEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9323..10858
                     /locus_tag="OUB02_001874"
     CDS             9323..10858
                     /locus_tag="OUB02_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCT8885633.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMKNGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKFQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10874..11635
                     /locus_tag="OUB02_001875"
     CDS             10874..11635
                     /locus_tag="OUB02_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885634.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11973..12842
                     /locus_tag="OUB02_001876"
     CDS             11973..12842
                     /locus_tag="OUB02_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8885635.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13171..13422
                     /locus_tag="OUB02_001877"
     CDS             13171..13422
                     /locus_tag="OUB02_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HCT8885636.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13534..14313
                     /locus_tag="OUB02_001878"
     CDS             13534..14313
                     /locus_tag="OUB02_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8885637.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14448..15098)
                     /locus_tag="OUB02_001879"
     CDS             complement(14448..15098)
                     /locus_tag="OUB02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885638.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15095..15598)
                     /locus_tag="OUB02_001880"
     CDS             complement(15095..15598)
                     /locus_tag="OUB02_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8885639.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILSNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15647..16162)
                     /locus_tag="OUB02_001881"
     CDS             complement(15647..16162)
                     /locus_tag="OUB02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT8885640.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16442..16969
                     /locus_tag="OUB02_001882"
     CDS             16442..16969
                     /locus_tag="OUB02_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8885641.1"
                     /translation="MHEQIDYCERKKIRTINIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            16953..18356
                     /locus_tag="OUB02_001883"
     CDS             16953..18356
                     /locus_tag="OUB02_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8885642.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFIFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         7120 a         1973 c         3342 g         6116 t
ORIGIN      
        1 ttttattctc aaaatatgca tattaattta ataaaatata ccaaaatgga aataaaaaat
       61 aaattttatg agaatttaat aactaataat taatatattt aggttataat aaatatgtgc
      121 gtaaaatcta acaataagca ataataccgt aaatttagga ggaataatat ggatacttta
      181 aaggaacttt ttaaaatagg aagtggacct tcaagttctc atactatggg accacaaaga
      241 gcagcagaga gatttaagaa tgaaaaccca gatgcagaaa gttttagagc aatattatat
      301 ggtagtttgg cagctacagg gaaaggtcac ttaactgact atataattga aaaaactata
      361 gcacctaaaa aagtcgaaat tgtttgggaa gaagatatta taaaagattt tcacccaaat
      421 gggatgaagt ttgaagcttt agataaagat aaaaatgtaa cagctgaatg gactgtatat
      481 tctgtaggtg gagggactat agcagaagaa ggtcaaagaa acagtaaaag caatagtata
      541 tatcacttag atactatgga tgagatagta aaatggtgca aagaaaataa taagactttg
      601 gtagattttg tgttggaatg tgaaccaaaa gacataaaag attatataaa aactataaaa
      661 gatgctatga gaaaatctat tgatgatgga ctaagtacag atgaaataat accaggcaag
      721 ttattattaa aaagaagagc aagtacattt tataatgcat ataaaaaaga taagagcttc
      781 tcaacacttg tatatgcata tgcactagca gcttcagagc aaaacgcttc tggtaatata
      841 atagttacag caccaacttg tggttctgct ggagtaatac caggaatatt ctttgcaatg
      901 caggatttct ataattatga tgacgaaaaa ataattgaag cactacttgt agctggaata
      961 ataggtaata taataaaaac taacgcttca atatctggag cagaggtagg gtgtcaaggt
     1021 gaagttggag ccgcttgttc tatggcagca gcagcagtag catacttaaa aggtggaact
     1081 atagaccata ttgaatatgc ggcagaaata gcacttgaac atcatttagg aatgacttgt
     1141 gaccctgtat atggatatgt tcaaattcca tgtatagaaa gaaatgcaat ggcagctcaa
     1201 agagcttatg atgcagctaa ttatgcatta ctaactgatg gctcacattc tgtctcctta
     1261 gaccaagttg ttgaaacaat gaaagaaact ggtatagata tgatggataa gtacaaagaa
     1321 acagctaagg gtggacttgc taaacatttc ttctcttgct aaaaataaaa ttttccaata
     1381 ataattcctc tgttttgatt tatgaaacag gggaattatt attaatggtg aattatttaa
     1441 taatgcaaaa tatttttata ttcaatttag ctttttattt ttaattagta taacatgcta
     1501 tataatattt taattataaa gaaatataga aatatttttt aataaattag aaattggagg
     1561 agattacatg agagaggcat catatttaca agaacaactt tataaacata ggaagtcatt
     1621 aaatcctatt gcagagattg ggagaaaaga gtataagact tcaaaatata taagagatta
     1681 tcttgataaa ttaggaattg agtatgaagt ttatttagaa acaggtatag taggtgtaat
     1741 agaaggtaaa agacctaaaa aagatattgc ttttagagct gacatagatg gtcttcatag
     1801 tgaagaaggg gtaaaacatt tatgtggtca tgatggacat gctactatat tacttggatt
     1861 aattgagtat ttaaatgata ataaagaaga cttaaatgat aatatagtat ttatattcca
     1921 accagcagaa gaaggtcctg ggggagcaaa agctttaata gatgaaggag tgcttagaaa
     1981 atacaatatt gatgaaatat atgggcttca tatatatcca gagttaccag aaggttatgt
     2041 aggtacaaga gagggctatt ttatggctca aataggagat attgacatag aaatagtgtc
     2101 taaaagtgga catggggcta tgccacaaaa tggtattgat ggtatagtga tagcttcaaa
     2161 ctttgtaagc tcacttcaaa ctatagtgtc tagaaatata agcccaatag acaatgcagt
     2221 gttgactatt ggtagaattg aagggggagc tagaagaaat ataatagctg aaaatataaa
     2281 gatggaaggt actatgagat gtttcagccc agaagtgtat gagaaaatga agcttagagt
     2341 tagagaactt gctagagggt ttgaattggc ttataactgt aaggttaata taaatattat
     2401 agatgattat attgctgtta aaaatgacaa ggatttgtat caagagtttg tagaggctat
     2461 tggaagtgat actgttgtag agttagagcc acttatgata tcagaagatt tttcttatta
     2521 tcaaaaagaa gttccaggat tattttttat gttgggttca agaaacgaag aaaaaggatt
     2581 tgtaaatggt cttcataata taaactttaa ttttgatgaa aagatatgtg taaatgcatt
     2641 gaatgtttat tcaaaattat taaaatacaa agaagcaata gattaggaaa ataaattata
     2701 aagattaaaa agcataaata cctaaattta aaaaaagtgt attaataaat accccttaca
     2761 tatttgtata aatttatttt aattgacaaa aatacaaata agaaggggtg atttttttga
     2821 aagaaaaagt acaaagagaa aagaagataa ctaatggcaa gaatactaaa atattgacaa
     2881 atgatgatat aatgaaatat gagatagcct cagaactagg tcttatggat aaagttggtg
     2941 aacgtggatg ggcaggactt acagctaagg aagcaggaag aattggaggt atgcttacat
     3001 cacgcaaaaa gaggcttaaa aagattgtgg aggagcagaa taaaaaggat gagtcaatat
     3061 agtgattttg cgtttgtata tgatgaactt atgaatgaag ttgattataa tggttgggtt
     3121 aaatatatag aagatataat agatagtgaa ggtgtaaaag taaaaaatat attggaattg
     3181 gcatgtggta cgggtaatct tacaattcca ttggctaaaa aaaattacga tatagctggt
     3241 atagatatat ctgatgagat gcttagtgta gcaagagaaa aggcagaaaa agaaggagta
     3301 gaattagttt tacttcaaca agatatatca gagcttgatt ttgaaatatc tgatttggac
     3361 tgtgtacttt gtgcttgtga tggatttaat tatataactt atgatgatga tttggaaaat
     3421 gtattttcaa agacatatga attattaaag aaagatggaa tattcatatt tgatataagt
     3481 tctttttata aattagccaa catattggga aataacatgt atggagaaaa tagagaagat
     3541 atagcgtata tgtggcaaaa ttactttgat gaagaagaga atctaattga aatggaactt
     3601 acattcttta ttaaagatga atgtggaaag tttgaaagat ttgaagagat acaccaacaa
     3661 agagcatata ctgaagatga gatagttgat atgcttgaaa aatctggatt taaggatata
     3721 aaatattatg gggattttac atttgacaaa cctagtgaag aaagtcaaag aatcttcttt
     3781 gtatgtaaaa aataagatat tcttgactat ataagataat tattaaaaat aaccatgata
     3841 taaaaatata gaaaatgcag ttttatagat taaaagtaaa gtgatgatac tttatgactt
     3901 ttatttttta ataattataa agttctatag ataaaaatta aaatatagta actattactt
     3961 agtaaaatta aaataaaatg ggttttgccc aaggttaaga tagataagta tttatagata
     4021 aattaaggta gtaatataat aaaaataaga caattaacgg gacatggagg aatattaatg
     4081 agagattatg tactaagagc gacatcagga aatggtcaag ttagagcatt tgtcgcaact
     4141 actagaaata cagtagaaga agctagaaga ttacatgaaa ctacaaaagt agcaacagca
     4201 gcacttggaa gaacattaac agccacatct ataatgggat taatgatgaa aaatgatagt
     4261 gataaattga ctgtaataat aaaaggtgga ggtccaattg gaactattat cgccacatca
     4321 gactctaagg gaatggtaaa aggatatgta ggaaatccac aagtagaggt tgaagactat
     4381 ccaaatggaa agttaaatgt agcagcagca gtaggtacag aaggtgttgt aaaagttata
     4441 aaagacttag gtcttagaga accatataat gggacttatc cattggtaag tggagaaatt
     4501 gcagaagact ttacttatta ttttgctgta tcagagcaga ctccatcagt agtagcttta
     4561 ggtgttctta caaaagaaga tgaagtagag tttgctggag gatttatagt tcaattaatg
     4621 ccagatgcag aagaagagac tattgctaag ttagaggaaa atgtagctaa attaccttct
     4681 ataactaaca tgttaaaaga aggaaaatca ccagaggata tattaaatat tgttttggat
     4741 ggtttggaac ctaaaatact tgatacctgt gaagttgggt ttatgtgtga atgttcaaaa
     4801 gaaagagtaa aaactgcttt ggttgcaatt ggtaaaaaat cattggctca aataatagaa
     4861 gaagataaaa aagcagaagt tggttgccag ttctgtaata agaagtatat gtatagtgaa
     4921 gaagagttgc ttgaaatttt aaaggaaatg tagactataa aaatagttgt attgatataa
     4981 gtaatgttat agtaatatag taaagtcgta ttgatatagt aattaaagta taatttatta
     5041 ataatagaca tatttatatt aaaaaataag tttaataaat ttttaagctt aataaatttt
     5101 aagaattaag aagctttctg gctgtaatgt ttgcaatgtt gcagttagaa ggctttttta
     5161 ttttattata gttcgtgtca aagattgtat gaaacaataa aatattgtaa aataatttgt
     5221 atatttagac ttgatttaca caaaggttta taagatattt gagttgatta tacgaaattc
     5281 tatgaagtag taaccctgtg actttaatca tgggaggttc agaagaagga gaaaatttta
     5341 aatatggaaa gtaaaacgaa gtacatagga ataagtatat taatattatt gatgatattt
     5401 ttaattgcaa agaattttaa cggaaaactt gaaataccta ttcctgagag taatagtaga
     5461 aaatttaaat cagcagcagt gttttatcct caacatcaag atgatgaggt tttatgggga
     5521 ggaagtgcca ttgtagatgc tataaaacag tgtggtgttg ataatgtata tgtggtatta
     5581 gtatcagatg gttctggagt taacgttttc aaagctaaca caaaatttag aaatttaact
     5641 aggaagcaaa aggaagaact tagaaataac gagtttaaat cagctttgag agagcttgga
     5701 gtgaaacctc aaaatgtgat tatacttgca gatatagata aaaaggaagg catacattat
     5761 gaattaatgg aaaagactat attggatttt gaacataagt ttaaaggcaa tgtgacccat
     5821 atagcccatc actataagta tgatgaccat ataatgcata gaaagaatgg ggaagtttta
     5881 aagaaactta aacgtgaagg taagataaaa gatgacttgt attttgttaa gccaaaatat
     5941 gtaaagtata taccaagaaa tgaaagagct atctataaag caatggattt tgatgactac
     6001 aataaggtta agagtgcttg ttatgaatat aaggttgtaa attcaaagcg taaaatgtat
     6061 ggtataggat atatttcggc acatagttat tttgaacatc ttctaaatga ccctgaactt
     6121 acgtctgtat taagtgaaga ttaatttctt ataagtagta agttatgttt gataagttga
     6181 ttggaatttt ctatagtttt gaaggtattg gaaatttagt tttatttaaa gatttaaaga
     6241 tagaggtttt tagtgtttaa aattttaaag ttaagttgag taattgtata gaattatttg
     6301 tgaaagaaga aacagtgtaa aacatagtat cttatttatg gttttaaaat cataggcaag
     6361 atactttttt tatgtaacta ataccatttt gagattatta acttaagaat tttatataat
     6421 ttaacactca tatataatga tgatatttag gtagaataga tgcaattacg cttaaataaa
     6481 ataactgtat tttttataaa aatcaaaccc tacactatac taccagataa aatctacatg
     6541 ctataatttg acctgtgttt ataaaaaaag gagtaatcta ataaaatagt atttcactta
     6601 tgtataacta acaactatta ttacgatttt ctctaaataa tttaaagaaa aataatgcag
     6661 gaggtaaaat atgaacaatg aatttataat tttaaaaggt tgcaaagaga acaatttgaa
     6721 caatatttcc ttaaaaatac caaaaagaaa aataaccata ttcacagggg tgtcgggttc
     6781 tggaaagtca tctattgtat ttgagacaat tgcaaaagaa tcacaacgcc aactaaatga
     6841 aaggtttagt acatttgtaa gaagtttttt acctaagtat ggagaggtta aagctgattg
     6901 tatagaaaat ttgtcaacac caattatcat agaccaaagt agacttggtg gaaattcaag
     6961 gtcaacccta ggtactatta cagatgtaaa ttccttttta agagctttat attcaaggtt
     7021 tggtagtgag tatataggta atgccaatat gttttctttt aataatattg atggtatgtg
     7081 cccacactgt catgggcttg gaaaaaagct agttcctaat atgaaccaaa ttcttgatat
     7141 gaataaatca ttaaatgaag gtgctatatt gctttcaggg tttggagtgg gttcttggca
     7201 ttggaatata ttcgctgaat caggattttt tgatattaat aagaggatat gtgattattc
     7261 agaagtagaa ttggaaaagt ttttgcatgg taaagcagaa aagattaaga tagaaaatgc
     7321 tggtcaaact aacatgacat atgagggact aatggttaaa tttaatcgcc tatacttaag
     7381 tagagaggga gaaatatctg aagctactaa gaaaaaattg agtaaacttc ttatagaaga
     7441 tagatgtcct atatgtgatg gaagaagact taatgaaaga gtatataggt ctttaattaa
     7501 tggttataat atagcagatt taactagtat gcaaattgat gaattggctg aagttattaa
     7561 aagtatagat gaggcagaag cacaaccttt aattaaagga ataatagaaa agttaaacag
     7621 tatcatagat ataggtcttg gatatttaac actggataga gagacatctt ctctatcagg
     7681 aggggaatct cagcgtataa agatggttaa gtatttaaat agtaatcttg tagatttaat
     7741 gtatattttt gatgaaccaa gtgtaggact tcatccaaaa gatgtgtata aattaaataa
     7801 tcttttaaaa aaattgcgtg ataaagggaa tacaattata gttgttgaac atgacccaga
     7861 tgttattaag attgcagacc atattgtaga tgtaggacca aaggcaggta agtatggtgg
     7921 agaagttgtg tatgaaggca gttatgaaaa tctacttaca agtggtactt tgacagggaa
     7981 tgctttaagt aaatttcttc ctattaaaga aagtgtaaga gaacatagtg gatatttaga
     8041 agttaaaaat tgtaataaaa acaatttaaa aaatgtatcg attaagatac ctaaaggtgt
     8101 attgacttta gttactggag tagcaggagc aggtaaaagt acgttgatta aggatgaatt
     8161 tttaaagcaa aatcctagtg ctgtacttat agaccaaagc cctgtttcag caaattcaag
     8221 gtcgagtttg gcaacatata gtggaattat gaacaacata agaagcatat tttcaaaaac
     8281 aaatggcgta aatgcttctt tattcagttc aaattcagaa ggagcttgtg ataactgtaa
     8341 gggttctgga atagtagaaa tgaatcttgc ttttatggag agtataaaga gtacctgcaa
     8401 tgtttgtgaa ggtaaaaaat ttaaaaaaga agttttagaa tataggtttc aagggaagaa
     8461 tataattgaa gttcttgaaa tgtctgtttt agacgcaatt gagtttttta atttaaaaca
     8521 gattaaaacg aaattacaat gtattgaaaa aatgggtatt ggatacctta ctttaggaca
     8581 aactcttgat actttgtctg gaggagagtg tcaaagatta aagcttgcta gtgaattaaa
     8641 taaggaaagt tcagtgtata tattagatga accaacaaca ggtcttcata tggctgatat
     8701 tgagaatttt ataagtataa ttgaagagat tgtagataat ggaaatacgg tcataataat
     8761 tgaacacaat atagatgtaa ttaaaagagc tgattggatt attgagctgg gacctgaagg
     8821 aggaacaaaa ggcgggagag taatatttga aggtattcca aaacaattat gtaacaataa
     8881 aatatcatta acagcaaaat atatagtata ataaataaaa tataataaaa ttataatgat
     8941 attttaataa atataatggg tatataaaat gataattaaa cttaacagta ataaataatg
     9001 tgagaaatat tcaaatttaa ttaaataaag tgtcagaaat ttgtttaaat tttagtattt
     9061 taacggttct gacctttttt cttattttta tggagaatat cgaggatgga gtctctaata
     9121 gttacttttt tgtgtctact tgcaagtttg attaaaaaat ggctatacac acttgtgact
     9181 tcagtcatgg gaggttccga ttataatctt aatataattt gtgaaataat ttgatgacga
     9241 tttgctgatg tataaacaat aaagattaac tacattaaaa tttaaatctt gttaagtaaa
     9301 acttataaaa ggagagagat ggatgataaa tgatattcta accttattat ctgaaagaag
     9361 tgattttttt ataaagctat ccatagaaca tttgatgatt tctattattt ctataataat
     9421 tgcgattgtt ttgggtggta taattggtat tattgtaagt gaatttcata attcttctaa
     9481 acctatattg ggaactatca attttctata caccattcca tctatttcta tgctaggatt
     9541 tctgatacca ttttccggaa ttggaaatac tactgctgtt attgcactta ccatttatgc
     9601 attattgcca atggttagaa atacatatac tggaattact aatgtggata aaaatattct
     9661 tgaagcagct aggggaatgg gaagtacaga ttttcagata ttgtataaaa taaaacttcc
     9721 gttagcccta cctgttatta tgtctggtat tagaaatatg gtgattatga caattgcatt
     9781 agctggtatc gcttcattta taggtgctgg aggcctaggt gtagccatat atagaggaat
     9841 cacgacaaat aacacaataa tgacaattac tggaagtata cttattgctc tattagcttt
     9901 aatatttgat tttattttag ggctggtaga aaaatttttt caacgaaaaa gaaaaaagaa
     9961 aaatagaaaa aaagtttttg ccattctagg aagccttatt cttatagcaa ctatctttac
    10021 gtcttattta tttaatcctt caaagactca aactattcat attgctacta aacctatgac
    10081 tgaacaatat attcttggtg agatgttgga tattttaatt gaaaaaaaca caggtctaaa
    10141 cgtggaaatt acacaaggtg taggaggggg aacatctaat atccagccag ctatgaagaa
    10201 tggagaattt gacatttatc cagagtatac aggtacaggc tggaatatgg tacttaagaa
    10261 aaatgaatta tatactgaaa gtatgtttag taaactacag tcggaataca aaactaaact
    10321 taaaatgcaa tggactggta tgtatggttt ttctaatagc tatgcattag cagttagaaa
    10381 agaagttgca gataaataca atttaaaaaa tataacggac ttaaaagatg tatcttctaa
    10441 attgacattt ggagctgaat atgactttta tgagagagag gatggatata ctccattgtg
    10501 taatacatat ggactgaact ttaaagatac agttgattta gatattggat taaaattcca
    10561 agctatcaat gaaggtaaga ttgacgtaat gcctatattt acaactgatg gtcagtatag
    10621 tgtttcagat gttgtagttc taaaagatga taaaaatttc tacccatctt atcaatgtgg
    10681 aaatgttgtt cgtttggaag tattagagga aaatccagaa ctatcaaagg tatttaagat
    10741 ggttggagat attttgactg ataaagaaat ggctaaaatg aattatgacg tagaaaacaa
    10801 caatttagaa cctagagaag ttgctcgtga gtttctgaat aagaaaggtc ttttataaat
    10861 tggaggaaat ataatgacac ctattataca atttaaaaat attaaaaaac aatataatga
    10921 taaaactata atagataatc ttaatttaga tatagaaaaa ggagagtttt tgactgtaat
    10981 tggttcatct ggttctggta agacaacact cttaaaaatg attaatggtc tgattttgcc
    11041 agatggagga aatattttga taaaccaaac agatatcaaa aacgaggact taattaaatt
    11101 aagaagaaga ataggatatt gtgtacaggg aagtgttctt tttcctcata tgactgttga
    11161 agaaaatatt tcatatgtac caaatctcct aagtaaaaag aataaattag aagtaaaaag
    11221 tgctgtaaac aaatggatgg aaatagtggg attgccaaat gatatgaaag tgcgttaccc
    11281 atcagaattg tcaggtggtc agcaacaacg tgtagggatt gcaagagctt tagcttcttc
    11341 tccagaaatt ttattaatgg atgaaccttt tggtgcagtt gatgagatta cacgaaagca
    11401 attacaaaaa gagataaaag aaattcataa aaaaacagga gttacgatta tctttattac
    11461 gcatgatatt tatgaagctt tgattttggg aacaaagact ttggtattaa atcatggagt
    11521 tgttcaacag tatgatactc ctgagaatat tttaaatact cctgctaacc aatttgtaga
    11581 ccagttattg aatataagaa aatctataat agatgatgat aatttaaaag aataaattta
    11641 aatttaaaat caaattgttg tgataaaaac tattgattta gagctaactt caagttgtat
    11701 acttgaagtg gaaaagttag attaaggtat agaagggaat ttagcagttg gctgtagaca
    11761 aggtaactgc tgaaggtgat ttcaataaat ggctagaacc tgtatctgtt gaagattata
    11821 ataaattgaa ataagaagct caatatacag agtagatagg cacttcaaat agaagtgtct
    11881 tttgtgtgcc ataaaataaa tcaaaatatt agcaaagaat gtgaaaaaca aaataaaata
    11941 atatgctatc tttaaaatag gaggggtgct atatggataa caatttgatt accattgaaa
    12001 aaactttaga ttatatagaa aaacatcttt tagatgagat gttagattta aatgtaattt
    12061 ctaaagagat aggctattca aaataccatt tacatagaat gtttacatca gttgtcggat
    12121 ttagtgtaca taattatata caaagacgta gacttactga agcagcgaga atgctaattt
    12181 ttacagaaaa accaattatt gagattgctt taatctcggg gtatgacaca caacgctctt
    12241 tttcaagagg gtttaaaaaa atgtttaaac gtagtcctaa tttttttaga agacatcaag
    12301 attttttacc actacaatta aaatttgata ttcataatag aaaaaaatta cgtggagata
    12361 tggttttaga tgttaaaaaa gttaattgtg cagatataaa tattgttggc tattctggta
    12421 aagtaagaaa aggatttcat attattggaa aatgttggag ggatttgcat aaaaataaag
    12481 agatgataga aaataaggta gatttaggtt ttttaattgg agttagtgat tatagcaatt
    12541 ttaaagagat tgagaatact cctacattta catacattgc aggtgctcaa gtagctactt
    12601 ttggcaaatt acctaatgga atggaaaaat tcgtattacc gaaaagtaat tatattgttt
    12661 ttacttttat tgggaaaaat gaggatagta tgcaaaatgt tgtagaatat atctatcaag
    12721 aatggttccc taattcaagt tgccagttta atgaaaataa tttatatgac tttgtaaaat
    12781 atggagaaaa aactgatgag aatggtttga gtgaaattca gttttgggtt ccaatcattt
    12841 aaaagaaggt attatgtata aagtagatgg atttatatag gcaaagtttt acagcagtat
    12901 gtatttataa ataatttata gtttgtagga aagaaataat tttactataa gattatgata
    12961 aaatacacaa tactatgaca aaatagtgtt aaataataga tttaattttg cataggaaaa
    13021 gttctataat ggtgtgtatt cataaataat ttataatctg taggaaagaa atagttttac
    13081 tataagatta tgataaaata cacaatacta tgataaaata gtactaaata atagatttaa
    13141 ttttgcaaag gataaggtgt tttaataaaa atgaatgtag aaaacgaatt acttaaaaac
    13201 ctagatagat tgcatacaac agagttaggc gttgtaagaa ttaagaaaaa tctttctttg
    13261 gagacaaatg atgtagtaaa ctggtgtaaa actaaaatag aatctccaaa tgccattatt
    13321 aatagaaagg gaaaaaactg gtatataagt gtatatgatt gtattataac agtaaacgct
    13381 cacagttata caattattac agctcataaa gaaaagaaat gaattgcaaa atacaaaggt
    13441 caaaagtatt ttccactgtt tgtagtacct atgaagaagt atataaaata atattacttt
    13501 tgaataataa tttaaatttt ggaggttgaa aaaatgaatg atactgtaaa agaaaaattg
    13561 gcagagtcac taacttctga tactactgat attttacctt atttgcctta tttattacaa
    13621 gatttttggg aattaggaag ttcaccagaa caaatgttac aattatttag aaagcatata
    13681 ccttttaatg aggatacaaa agttttggat ttagcttgtg gaaaaggtgc tgtatctgtg
    13741 aagcttgcaa aagagttgaa tatacatatc aaaggtattg atttgatacc tgaatttatt
    13801 gattatgcga gaagaaaggc tattgaatat aaggttgatg ctttgtgtga ttttgaaatg
    13861 aatgatgcta atagttctgt aaaaattgag cacgattatg attgtgtgat ttttggagct
    13921 gtaggggata ttttaggtaa ttataaagaa actataagta agctattacc tacaataaaa
    13981 agtaaggggt tcattttaat tgatgatgca tatgtagtag ataaatctac aaatgaacag
    14041 cttaattttg agagggtgta tcccacttac aatgagtggt gtatattgtt caaagaatta
    14101 ggtctgaaac taattgaatg tataccatgt ttagaacttg aaaatgatgt tgaagaggaa
    14161 atgaactgga ttataactcg tgcaaatgag ctaattaaaa aatatccttc tcaaaaagat
    14221 atgtttgaac aatatgttat tagccaaaaa aatgaatata ctgatttaca gcaggacctt
    14281 gttggagcaa cttggatttt acaaaaagaa taagataaat attagactag ataatcaatg
    14341 ttgtagtgaa ttatcccatg tctagtgaat ataaataaca taaaaaaccg tcaagatgaa
    14401 tagtttattc gtgcttgacg gtttttagta tttgttgaag taaaatttta tttctttagt
    14461 atatcacttt tagtcaatcc atttgattta gcctgagtat tccattgatg attccccata
    14521 agtttatata ggaatctagg gaagttaggt gaaataaaaa tagtactttt gttggaatca
    14581 tctatatgta tattatcact aagtatattt tttgaaagtt cttttaatgc aattcctaaa
    14641 tcttttttag gtcctttacc caaaggtacg gattctagtg aaccaaacat ctccccagct
    14701 ccaattccaa taccttgaac ccatgtaaag ttgatttttt tacaccaatg ctctatcatt
    14761 tgaattgcca aatgattatg ttcaccttca ttgaatccac agttaactat tgcatacact
    14821 ggaataggtt ttttattttg tgtttttaaa aatttttcac atttctctaa aaaatgaagt
    14881 aaatgagacg gaataccatc tacataaaga ggaaatgcta ttattaatct atcacaatta
    14941 agtatggcat taagagcgtc aggatactta gatgtgctat ctactttaaa tgtaaaaact
    15001 ttattatcat tttgaataag ttgttttaat tcagataaaa aatactgtga attattttta
    15061 tttacttttg gactaccatt tatcattact atattcatat ggattcacct cttatatcct
    15121 gtatattttc atagaaacaa acagagtgtt cttttatatt gaaatttata gcatttccat
    15181 ttactaattt cgtagcagtt tctttttcag cttctgtaat acaatctcca tagaatatag
    15241 cttttaaatt tatctgttta tcatatctgc ttttatgacg catcatatta tcttttatta
    15301 caaagtatgg aagaatataa ttaatgcttt tatcaagtac gtttttgaca aaagggctaa
    15361 accctccgta acagcatctg cttattataa taattccatc agattcattt ataagtttac
    15421 ccatgttgct gtaataatct tttacaacac attcagaagg agttttaatc cagcaaccaa
    15481 aacaaccaat acatttatgg atttcttttg tgtttgaaag tatttttata ttgtcatcaa
    15541 gtttgggtaa taacttttct aattctttgt cagttaaatc atgtattaaa agcctcataa
    15601 agtccatctc cttttattat gtatgtttac ttctaaaaat tatatcttat agatttttca
    15661 gtaaatcttt acaccaatta ataacaaaat tataatagtc aactccataa atcattgttg
    15721 catacatata tgtatattgg tcaacacaat gattttcttc tgcatcattt ttttgtttac
    15781 ttaatttatt tagaagtaat tctacttcag ttattaacat ttttaagttt gtttcaatta
    15841 tttctttggg tagatacttg taaaagaata tattaacaag gtggtcttgc tttgttttag
    15901 aaaaatttat aggtttcttt aaccattcaa gaaatacttc tttaccagta ttagttatgg
    15961 aatatagttt ttttaatttg ctaccatcta taacttcgtg atagtgtata tatccttttg
    16021 tttctaatct tttaagtgca ggatagatac ttccaaagct ggcgtcaaaa aagtacgagg
    16081 tactttctga cattatttgt tttaagtcgt atccacttaa ttctttttcc attaaaaatc
    16141 ctaaaataat gtattctaac atattacctc ctgatatcaa ttagatatat atcgattcga
    16201 tatatttatt attacagata ataaatctta cgtcaagatg ctttttaaaa taagctaaaa
    16261 agaattagga gttattgata aatatgcaaa aaaattaata taaaaggtaa ttaaatatca
    16321 atgaagattt aaaaatagtt aaatcaaagt aagagtctaa tatataaaat ttatagtaac
    16381 tttatggttt aaatataaac ttgtgtgcta ttgacaaagt ataaaattag cagtaaaata
    16441 attgcatgag caaatagatt attgcgaacg caaaaaaata aggacaataa atattaaagg
    16501 agagaatatg agagagctat taaaatgggt ttccattata aatagataca atacaaatta
    16561 tattgacagg aaattggcgg atattggaat caataatagc caatattttt atgtattaca
    16621 catatgtaat aatccaggta ttacacagga cactatattt gagaaaattc ttgtgaatcc
    16681 tagtaatata acaagagctt tggcaaattt agaaaaagaa ggctttataa caagagaacc
    16741 aggtgtaaag gataaaagaa catggcattt atatcctaca gaaaaatctt tagaatgtta
    16801 tgatgaaata ctaaaaataa caaatgggta tgttgaggag ttgttgagtt catttgagaa
    16861 agaagagcaa gagttgttta tgtcaatgtt aaagaaagtt gcatttttgg ctattgatat
    16921 gaatgaagaa gcaaaattgg aggcgaagga aaatggaaga aaaatataag gagccattgg
    16981 aatatgaaaa ggtttcaaag ttattaacta agtacgcaat tcctagtatt atagcaatgt
    17041 tagtaagtgc tttatataac attgttgacc aaatatttat tgggcaaggt gttggtgtat
    17101 tgggtaatgc tgctacaaat gtcacttttc cactaacaac aatatgtacc gcaattgcat
    17161 tattgttagg aattggagga gcgtctaagt gttcattgga attaggtgct aaaaattcag
    17221 aaaaagctac aaaagcagca ggaaattcta tatgcttgat ggctgttttt ggaataagtt
    17281 tatttatagt agtatcaata tttttaacac cactattaaa attctttggc tctacatcag
    17341 aaatactacc ttatgcacac acatatacaa aaattacatc aattggatta ccatttctta
    17401 taatgtccac tggaatgagt aagttgatac ttgcagatgg gagaccaaaa gcttctatgt
    17461 tttgtatgtt aataggagca gttattaata caattttgaa tccattattc atttttattt
    17521 ttaatatggg aattgctggg tctgctttag caactgttat tgggcaactt atatcttttg
    17581 gaataagtgt tcattatata aagaattttc aacatattaa attacataag aaaagtttta
    17641 aattagatag tataatttat aaacatatat ttgcgctagg ttcaagctca tgttttaatc
    17701 aattagcaat gactgtaaca cagatagtta tgaataatac attggcatat tatggggcta
    17761 ggtcagttta tggaagtgag attccacttg cttgtgtcgg tatcattatt aaagtaaata
    17821 tgatttttat atctataatt attggtatat cacaaggtat gcaaccaatt atagggtata
    17881 attatggggc tgaaaagtat aatcgtgtaa aagaagcata taagtttgca gtgggaagtg
    17941 caactattat atctgttatt gcattcttat gtttccaatt cttcccaaga caaattacgt
    18001 ctatatttgg taacggaaat gagacttatt ttatatttgc agaacattat tttagaactt
    18061 tcttgttctt aacatttata aatggtgttc aaatagtatc atcaactttc tttacatcaa
    18121 ttggtaaatc agtgttggga ctttttactt ctttgactcg tcaaatttta tttttaattc
    18181 cattgatagt aattttacca atgtttatgg gaattgatgg agtaatgttt gcaggaccaa
    18241 tagcagatgc agcagcggca atggtatgta tatactttgc agttcatgaa atgaaatcat
    18301 tgactataaa gcaaaataat ttgatgagta accataataa gattgcagag gtataagaca
    18361 atatttaaga agtatagaaa attaatttaa agaagtataa taaactaatt taggaagtat
    18421 agaaaattaa tctaaagaag tataataaac taatttagaa agtatagaaa attaatctaa
    18481 agaagtgtaa taaactaatt taggaagtat agaaaattaa tttaaagaag tataataaac
    18541 taatttaaga a
//
LOCUS       DAKBNN010000066        18101 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000066 DAKBNN010000000
VERSION     DAKBNN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.066"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..578)
                     /locus_tag="OUB02_001884"
     CDS             complement(<1..578)
                     /locus_tag="OUB02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885643.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPKETRELIQEISTKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMVKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQK"
     gene            complement(652..1191)
                     /locus_tag="OUB02_001885"
     CDS             complement(652..1191)
                     /locus_tag="OUB02_001885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885644.1"
                     /translation="MKNKQETVKKKRFLFMLVLFSCVYYFLIVKGKINLSILTNTTDL
                     SNHFNFITVNSVFVGFLFSSLSLILGLSGTSSIIRLEKADFMENIYRNISMGIFLSFI
                     SIICSLSMIFLKNYIIKIPLMFYIVVPSIELLSLLLTLIVFFKATRDVWFIIKIVRNK
                     IKKSVPSDDDLNETIRLIK"
     gene            complement(1222..2154)
                     /locus_tag="OUB02_001886"
     CDS             complement(1222..2154)
                     /locus_tag="OUB02_001886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885645.1"
                     /translation="MANRKVMYYCVELKNVSNGEKVSSEQMKVELNKIFDDKCISHEE
                     LKHLILKDKNDAFERITMDIISNEEDYLFGRVGKLKGNGDILLRNIDTNKANEIVEKN
                     KRPEIYTYFMLDYAHGILGYLNNQSLPKPDMLKRIFDLNNSCFEMNIKNVINPQTVRE
                     LMTPGSKLSKIEYSYKVPSIYVLQSLGLSRETVADLENTDYQTVTIQIASESRQKLTE
                     KSETIKKLIKHFSTNKNLENKVFKGKAPNDAMRNYKFDVDNYSLIIDVPRDKIEGGKT
                     KIYTLDEITEEVKIRMKEKYINNRKHILRLCGIE"
     gene            2297..2497
                     /locus_tag="OUB02_001887"
     CDS             2297..2497
                     /locus_tag="OUB02_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HCT8885646.1"
                     /translation="MNMMPVSSRRIRSVGWENNTLYVQFHNGVTYAYLNVSETEYQDF
                     LNSPSLGSELSRLDKIHPYHKL"
     gene            complement(2525..2686)
                     /locus_tag="OUB02_001888"
     CDS             complement(2525..2686)
                     /locus_tag="OUB02_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885647.1"
                     /translation="MEKKEIEELSKICEPILEYLNKKYTPHNSIVISTDGIKLLSIEI
                     GIPVKHQND"
     gene            complement(2753..3022)
                     /locus_tag="OUB02_001889"
     CDS             complement(2753..3022)
                     /locus_tag="OUB02_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885648.1"
                     /translation="MDMINLEEIFCKAKLLDKKYISLKINITEYPSEEIIIIQNTNFE
                     SKLRYYKEIYDYKLNHKYVDGIKIIDCTYGNDFKEIELYINKNLD"
     gene            complement(3161..3640)
                     /locus_tag="OUB02_001890"
     CDS             complement(3161..3640)
                     /locus_tag="OUB02_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885649.1"
                     /translation="MDKESIKEIVRELRKEEKENKKREVFHNTKLLLKHYNDLKSHIA
                     NSISDIEDVKSDYDDLLCLDIEDLYILSIKRSKAKTLIMVAHIDMALETLKVTQNKLQ
                     SLEKYKALELYFFKEKTYEDIAELLHCGVATVRRWVNEMVKELGIYLFGVDGLKIKL"
     gene            complement(3633..3770)
                     /locus_tag="OUB02_001891"
     CDS             complement(3633..3770)
                     /locus_tag="OUB02_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885650.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(3794..5164)
                     /locus_tag="OUB02_001892"
     CDS             complement(3794..5164)
                     /locus_tag="OUB02_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT8885651.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IYNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHKEKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(5113..5439)
                     /locus_tag="OUB02_001893"
     CDS             complement(5113..5439)
                     /locus_tag="OUB02_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCT8885652.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKPSKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(5731..8148)
                     /locus_tag="OUB02_001894"
     CDS             complement(5731..8148)
                     /locus_tag="OUB02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885653.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADI
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKINKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDGWERYDKRLRFGKIYGSQ
                     RAFIRKK"
     gene            complement(8160..10118)
                     /locus_tag="OUB02_001895"
     CDS             complement(8160..10118)
                     /locus_tag="OUB02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCT8885654.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(10118..10690)
                     /locus_tag="OUB02_001896"
     CDS             complement(10118..10690)
                     /locus_tag="OUB02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCT8885655.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(10713..11285)
                     /locus_tag="OUB02_001897"
     CDS             complement(10713..11285)
                     /locus_tag="OUB02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCT8885656.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(11338..11673)
                     /locus_tag="OUB02_001898"
     CDS             complement(11338..11673)
                     /locus_tag="OUB02_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885657.1"
                     /translation="MPLDAGIKDKLDESISIILDLKERKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVTVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDI"
     gene            complement(11688..12854)
                     /locus_tag="OUB02_001899"
     CDS             complement(11688..12854)
                     /locus_tag="OUB02_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCT8885658.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISEKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAGDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(12866..13303)
                     /locus_tag="OUB02_001900"
     CDS             complement(12866..13303)
                     /locus_tag="OUB02_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885659.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(13374..13517)
                     /locus_tag="OUB02_001901"
     CDS             complement(13374..13517)
                     /locus_tag="OUB02_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885660.1"
                     /translation="MLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNKKV
                     LVK"
     gene            complement(13504..13896)
                     /locus_tag="OUB02_001902"
     CDS             complement(13504..13896)
                     /locus_tag="OUB02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885661.1"
                     /translation="MDNTNEIVKRFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(14058..14240)
                     /locus_tag="OUB02_001903"
     CDS             complement(14058..14240)
                     /locus_tag="OUB02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885662.1"
                     /translation="MKHLMDALDANMIICVEGVQLPRGKSTLVDLLNENGYRAIKDCD
                     VFKIELNKPIQKTPQK"
     gene            complement(14376..15053)
                     /locus_tag="OUB02_001904"
     CDS             complement(14376..15053)
                     /locus_tag="OUB02_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885663.1"
                     /translation="MEFLWKKLINIINKIKQYRRNKKMKENNRIVSIGVDGEIDLTDK
                     ELEMVNNFKKIMNSINNKIGIDCGISLSDKDIEDNKNILDKIASKVAWHYKKMNFEHL
                     EMDEKFFDDFTFKFMLKFFGDIEEDELIKYLTYIKEESNELSARERIFYKIVDTLDTI
                     ADIKIADQNKIEKEVGMCMGEDNYITYCIDEDLIKFYIKDEEELVIDKNSPLLYMLNT
                     LFYEVHE"
     gene            complement(15025..15828)
                     /locus_tag="OUB02_001905"
     CDS             complement(15025..15828)
                     /locus_tag="OUB02_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCT8885664.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDVRNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EESYFNKKKDTLITNQLSPELQLFKQIFDTVAKQEIEQKQIKQDITETKQEIKSIKDV
                     VTLNTTDWREETRKLIVSMAQNLGGNEYINTLRKESYELLCKRFNVDLKRRLNNRRSK
                     MAEKGVCKSRREKFNYLDVIQEQNYLIEGYVIVIKDMAIKYGVFMEKTN"
     gene            complement(15834..16607)
                     /locus_tag="OUB02_001906"
     CDS             complement(15834..16607)
                     /locus_tag="OUB02_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HCT8885665.1"
                     /translation="MSNNLQVFKNSDFGEIRVIDLNGEPWFVGKDVAETLGYKDTSDA
                     LKRHVDDEDKGVGEIPTPGGNQNMKIINESGLYSLILSSKLLTAKKFKHWVTRDVLPS
                     IRKTGTYSSKKSECSKDDSEIKYMNAQTRLKNARAREAKIYLELADKVDIKEYKQLMY
                     SKTIELLSGETLIPLPKMERKTYSATDIGKMLGISANKVGGLAKALNLNVSTVSAKLR
                     NANRLKYIEAKNIQLMFFPNLSTEYLFEIEDEVKEKYRG"
     gene            complement(16619..16810)
                     /locus_tag="OUB02_001907"
     CDS             complement(16619..16810)
                     /locus_tag="OUB02_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885666.1"
                     /translation="MREKKILDTIPKLQTLTESELDLIITAVDSCVVVQTLMNTKSPK
                     DFTSKLSNLFEVGRENTKN"
     gene            16955..17335
                     /locus_tag="OUB02_001908"
     CDS             16955..17335
                     /locus_tag="OUB02_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885667.1"
                     /translation="MSNETISTRIRKIRKDANLSQPAFGEKLGVSKDVISNIEYNRVE
                     PKPLFINYMCDVFNVNKEWLLNGVGNMYISTEDDILLGEAFAYITTCENEKLKKIVIN
                     LCKLEDIYVDAICTTVDGIISDRK"
     gene            complement(17410..17604)
                     /locus_tag="OUB02_001909"
     CDS             complement(17410..17604)
                     /locus_tag="OUB02_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885668.1"
                     /translation="MDEVERVIILEEDCSREKFNIIKWILNDSNKSDLEIINDIQDIH
                     NIKYKNTIKSYIKNIKNKCS"
BASE COUNT         5348 a         3349 c         1781 g         7623 t
ORIGIN      
        1 tttttctgct cctctgtagc caaattccaa tccttatgaa tcatttcatc atactgttta
       61 attaaacttc taagttcact catagcccta ctctgtgcat taagaaaaga tgcttgccta
      121 tcccatgcaa attgaaattc atactctatc ttctcaccat tttctgtgct ttcatatttc
      181 tttaattctt taaccatttc ttccttgtct ttaacataca ttatcttttg tgctcttatt
      241 attgctgcat actgaattgt tatctgctcc caaagaatat caaatttatc ttttgtggat
      301 atttcttgta tcaattctct agtttctttg ggtagatact ttgagaagaa accaaacttt
      361 tcagcatttt tattacctgg tggaccagta gcatttttat tacctatggg tgcacccctt
      421 ttatttttag gtgcaccctt ctttttctcg ctagaccaat tgtatctttt tatccatgac
      481 tttaaagtgt ttaaactaat gtcatacttt gctgatattt ccttttgttt catacctttt
      541 atgtaatctt gttttacctt ttctttcaca tcttgcacac caccacctcg tttgtttgtc
      601 gttttgggaa taaaaaaaag aactcatttg gagttcttta actaattttt attattttat
      661 taatcttatt gtttcattta aatcatcatc tgatggaaca gattttttaa ttttatttct
      721 tacaatctta attataaacc aaacatctct tgtggcttta aaaaacacta ttagtgttaa
      781 taacaaactc aacaattcaa tacttggaac tactatataa aacataagtg gtatttttat
      841 aatataattt ttcaaaaaaa tcatacttag tgaacagatt atagaaataa aagaaagaaa
      901 aatacccata cttatatttc tatatatatt ctccataaag tctgcttttt caagcctaat
      961 tatagagcta gttccactca accctaaaat taaagataga gaactaaata aaaaacctac
     1021 aaatactgaa tttacagtaa taaaattaaa atgattactt aaatctgttg tatttgttaa
     1081 aattgataaa tttatttttc cctttacaat taaaaaatag tatacacatg aaaatagcac
     1141 aagcataaat aaaaatcttt ttttctttac tgtttcttgt ttatttttca tgtatttcac
     1201 actccttaag tggaatctat attattctat cccacatagt cttaatatat gctttctgtt
     1261 atttatatat ttttctttca tacgaatttt cacttcttct gttatttcat ctaatgtata
     1321 tatttttgtt ttaccaccct ctattttatc tctaggaaca tctataatca aactataatt
     1381 atctacatca aatttataat ttcgcatagc atcattagga gctttacctt taaacacttt
     1441 attttccaaa ttcttattcg tactaaaatg tttaattagc ttttttattg tttcactttt
     1501 ttctgtaagt ttctgtctcg attcactcgc tatttgtatt gtaacagttt ggtagtctgt
     1561 attttctaaa tctgcaacag tttctctact taatcctaat gattgtaata catatatact
     1621 tggtaccttg taactatact caattttact tagcttagac cccggcgtca ttaactctct
     1681 aactgtttga ggatttatca cattttttat gttcatttca aaacaactat tatttaaatc
     1741 aaaaattctt ttaagcatat ctggttttgg aagtgattga ttatttaaat accctaaaat
     1801 gccatgtgca taatctaaca taaaatatgt atatatttca ggtcttttat ttttttctac
     1861 aatttcattg gctttatttg tatcaatatt tctaagcaga atatcaccat ttccttttaa
     1921 ttttccaact cttccaaata aataatcctc ttcatttgat attatatcca tagttattct
     1981 ttcaaatgca tcatttttat ctttcaaaat taaatgtttt aactcctcat gtgagatgca
     2041 tttatcatca aatatcttat ttaactcaac tttcatttgt tcactactaa ctttctctcc
     2101 atttgagaca tttttcaatt ccacacaata atacataact tttctattag ccataaagat
     2161 tccactccat atatgatttt tattctataa ctaataaatt ctacatattt cattttattc
     2221 ctccaacaat cgttcgacaa tagcagaatt catcacaaat ttatgctaaa attttactga
     2281 aaggaggtgt tagcacatga acatgatgcc agtttcatca aggagaatta gaagtgttgg
     2341 ttgggaaaac aacactttat atgttcaatt tcataatgga gtaacttatg cttatttaaa
     2401 tgtaagtgaa acagaatatc aagacttctt aaattctcca tctttaggtt cagaattatc
     2461 aagacttgat aagatacatc cttatcataa gctttaatta acaagaaacg actaatctga
     2521 taaattagtc gttttgatgt tttactggta tacctatttc tatagacaat aattttattc
     2581 catctgttga aataacaata ctgttatggg gagtatattt tttattcaaa tattctagta
     2641 ttggttcaca tattttactc agttcttcaa tttctttttt ctccatgcta ctatcctcct
     2701 tctaaatact actagttcct tcaacctttc ttgctttatt ttctattaaa aattaatcta
     2761 aatttttatt tatatataat tctatctctt taaaatcatt tccatatgta caatctatta
     2821 tttttatacc atctacatat ttatgattta atttataatc atatatttct ttataatatc
     2881 taagtttact ttcgaagttt gtattttgaa taattattat ttcttcactt gggtattctg
     2941 ttatatttat ttttaaactt atatattttt tatctaacaa ctttgcttta caaaagattt
     3001 cctctagatt aatcatatcc attttttatt ctccttcgtt taaaaaagaa aaagactaag
     3061 tcggggtagc ttagtctttt taaaggggga atatattata cacttgtttc atactatcat
     3121 tataacttat ataaaataac aataaaatat catcatttta tcacagtttt atttttagac
     3181 catctactcc aaataggtat attccaagtt ctttaaccat ttcatttacc caacgtctta
     3241 ctgttgctac tccacaatgt aaaagctctg ctatatcctc atatgtcttt tctttaaaaa
     3301 aatataactc taaggctttg tatttttcta aggactgcaa cttattttgt gttactttta
     3361 atgtttctaa tgccatatct atatgtgcta ccataattaa ggtttttgcc ttacttcttt
     3421 tgatacttaa tatatataaa tcctctatat ctagacataa taaatcatca taatcacttt
     3481 taacatcttc aatgtcacta atagaatttg caatatgact ttttaaatcg ttatagtgct
     3541 ttaacaaaag cttagtatta tgaaagactt ctcttttttt attttctttt tcttcttttc
     3601 ttaactctct tacaatttct tttatacttt ctttatccac ttaaatcact ccttcttgct
     3661 ctcttcgaac aacttctact gcaatactta actttagatt tatccaactt ataaaatctt
     3721 ctaccacacc aagcacatct tactatttta cctgggctta taagttccat cttttctttt
     3781 tactttcata ccttcactcc ctatattctt ttaattttgc tttgactgct tcaagtaatg
     3841 cactttgtcc tttatcttta ttctctagag cttccattac ttgttcatct atggtgcctt
     3901 tacagattag atggtgaata ataacagttt ccctttgacc ctgtctataa agccttgcat
     3961 tagcttgttg atacagttct aagctccaag ttaatccaaa ccaaacaatt atactcccac
     4021 ctgattgtaa attaagtcca tgtcctgtac ttgctggatg acaaagtaat aattgtattc
     4081 ttccattatt ccaatcatat atatcttttg agttttctat tgttcttggt ttcaaactct
     4141 taaactcttt cattaaacga ttataatcat gtttatagtt ataaaaaact ataataggct
     4201 taccatttga aacatctata atttctttta aagcttctaa tttttcttta tggagttcct
     4261 ttacattttt atcattatca taaattgctc cattagctaa ttgtaaaagt ttatttgctg
     4321 ctacagctgc tgatgaagct gttattatat ctttttctaa ttctaatatc ttctcttttt
     4381 ctaattcttt gtaatattta agtattttac tatcaaggtg tatttcaatt ttattatcta
     4441 tttttctagg catattcaaa taatcttctg cttttaagct tatacaaata tcttttattt
     4501 tattatatat tgcattttct gctccatctt taggctgcca attatatata gctcctgttt
     4561 gataattttt ttgtcctggc tcaaagtatc gttctttata tcctgtaata gttttaccta
     4621 gtctttcacc tctatcaagt aagtacatct gtgcccataa gtctattaaa ctatttggtg
     4681 ttggtgtacc agtaagccca actactcttt tagttaaagg caatactttc tttaaacttt
     4741 taaatcgttg tgctttattt gatttaaaag aactaagttc atctatcaca accatatcaa
     4801 atggccattt tcttttataa aaatccacta tccagggtac catttctcta tttattatat
     4861 aaatatctga gtctgtactt aaagctctca ctctatctaa tttactacca agaactttag
     4921 atacttttag atgctttaga tgctcccatt tttttacttc actactccag gtatctcttg
     4981 caactcttaa aggtgctata actaaaactt ttgatatatc aaagtaatca tacattaatt
     5041 cacttatagc tgttagagca cagactgttt tacctaatcc catatctaat agtaaaccta
     5101 tattattatt atctatagtt tttctaattg tgtattcttg gtatggatgt ggtttaaatt
     5161 ccatccctta acctccttga taaaatcatc tatctctttt aatgtgctta cacacttaac
     5221 tttaaatcct aattctctta attctcgcat tttatattgt tgaagcttac ttggtttttt
     5281 acctggtgct ttaagttcta caaatataac atgtccttct ggtaataaga caatcctatc
     5341 tggcacacct gcctcccctg gtgaaataaa cttcatagcc tttccaccta acaactcaat
     5401 ctctttttta agtctttttt ctatttttga ttctaacaaa atatcacttc cttaaattta
     5461 agtgttacca atgttaccac gatttgtcta tatatacata tatacgtatt aggcatgtat
     5521 atatgtatac gtatatgtct aatattactt atactacttt atatataatt tttggtaaca
     5581 ttggtaacat tacacttgta tatattcaaa tcaatgcatt gtaaatgtta ccaagaggtg
     5641 ttaccaaagt catttttttg gtaacattgg taacatctct gaaaattaat atttcttata
     5701 taaatgatta tattttatac tttggtaaca ctactttttt cgtataaaag ctctttgaga
     5761 accatatatt tttccaaatc ttaatctttt atcatatctc tcccatccat ccaatccttt
     5821 taatatatca ttaatttctc ttgataagat aggcgtgagt tgtttaggtt ctccattaaa
     5881 tagctcaacc catatctcca taacacacgt tttttctctt aatattgtcc cttcttttaa
     5941 atcaccaaaa tctgaaccat gaatatattc tctcttttct gaaatactta aatcatacca
     6001 attcttagta ataggtttat ttagatactc ttcgataatt ccagatttag cattttcttc
     6061 tgagtgagtt ctttgttgtc tttcagcttc ttttttctcc tcatctgata gatatagctg
     6121 ttcattggct ttatataatt ctactgcttc tgcccaaatt tgatttcttt cattatcaag
     6181 ttggccatta aatatactct tgttaatctt ctttactcct gtatctattg gccaaaacct
     6241 tctattccca gtcttatctc ttaaaaattc tttatcatta gttgttccta taactacaca
     6301 ttgacgtaaa aatcttgagg ttctcttccc atatgcgacc ctatatatat cctctgattt
     6361 gcttagaaaa tgcttaactg cctcaatatc tgctttttta gtagccatca tttcacccat
     6421 ttccagcaac catactcctt gtaattgttc atacgcttct ttaccttgta cagtagttaa
     6481 actatcagaa taccagtctc caccaagttt tttaataaaa gtactttttc ccattccttg
     6541 aggaccagat aaaaccatca tattatcaaa ctttattcct ggattaaata ctcttgctac
     6601 tgctgctact aaaacttttc ttattatagt ccttgtatag tggttatctt ctgcacctaa
     6661 atagtcaatt aaaagtgtat ccactctctt aatgccatcc cactttaaag aatttaaata
     6721 atcttttata ggatgaaaag tattattttc aaaagcaatt attagagcat catttacttt
     6781 tgatggtgat gagatattgt aaattgtttc tatatgatgt ctaagcccag aatcatcact
     6841 atcattccaa tcatttaatt tatcatcttt tctccaaggt aacttaccta aaacgacagc
     6901 tctatttgaa aattcattat aagctatttt tcctttcaaa tatgggtcat tttctataaa
     6961 cattaagata ttgtttgttg ttttcttata acttccctta ttgtcataat ctaacctagt
     7021 taaccattca tcatcttcaa aatctatatc accaaaatca tccttagctt tatcaaggtt
     7081 ttctcttcct atagtctttc gtactttagt gtcactgctt gcaaattcgc tcattcgagt
     7141 aaatgaaggt aatctgttta caggcgtttc gggtttagca tcttcatcaa gttcaccaaa
     7201 tttatgtatc ctaactaaat caaaagcatt gcataaaatt ccgcttgctg ggtctgtacc
     7261 atgatgacta tatgaaaact tgtcatcata aataactact ccaccacttg tactaccttc
     7321 tgaatatgta tacctggttt catcaatacc aggaatatat acttcattta agaaagtttc
     7381 tatagtttct tttatactat aggacctgca aaatgcacct ataattccag ccttttcaat
     7441 agggtcttgt tgttttttta attgtgtatt aaacttttgc ctctccctcg aactttctgg
     7501 ccaatagctt acatctgtcc agtctaaata taaatctagt atttcatttg ggtttaaaaa
     7561 ctctccatct tgaaatttaa atatatagtc tccatcaatt gaagtacttg gaaaatacat
     7621 aagtctatga ggttgatatg tagtatcatc aaacatatct attcctataa tatctgctat
     7681 catcctggaa attgcttgat attcttctgg tagtactggt ctagttaaag gtattacaag
     7741 tctatatctg ggattattct cagtatgtga gtgcgtagaa tacataagac aagcataatc
     7801 atttaataat gttatatcct cccatatatc tttattcgca taatctatat ctaaagttat
     7861 aatacttcta tttgcaatat tctctgcttt tcgtcttcca ttttttaagc ttccacctac
     7921 aaatccacca acatctttaa ctctatcttt ttcagtcttt gacatcttcc tatattctgt
     7981 atatgtttct tgagttctta atgttttact taatctattt acaagttcgg accataaaat
     8041 acttttattt ttccaatgtg tttctaattt attttttcct atggccagca tgagttggcc
     8101 atcatgtctt acatttatgt gttcaatttc actggccttt atatccataa atcaatcacc
     8161 tagtctttct tataataatc acattcatat ccatctgctt taagaggaag acctttagcc
     8221 caagatattt cttttcccat aatactgtta acttcttcta aagaacctcc ttttttatct
     8281 acatcaatta caagctcatc atgtacatgc attacaatgc tataacctgc atctgtcaca
     8341 ttaaacatag cctctcttaa gcaatctcta gctgtagctt gaacaatatt ctcaactaac
     8401 ttaggtccat aagtatctat tcttttccat tgtttacttg tctgttccat accttcatat
     8461 gttatcttat ccccactaaa tgtagtatgt ggctctatct taggccttag atacgataat
     8521 cttctactac ttggtagttc tataaataaa acgcctggat tataaataaa tttaatccca
     8581 tgttgaattt ctactatagt cctatctttt atagcttttt tagctgcctt atctacatcc
     8641 caccaaaact tagttatatt tggattagca tttctccatg cagtaacaat aggttgaagc
     8701 tcttcttctt taagccccat tttaatagcc cccatagaaa ttaaggcccc tacacttccg
     8761 ccatatccat tcgataattc tgctagcttt cctttttgtc taagttcaga acctttttta
     8821 atattttcga ttggaatttt aaacatctga ctggcactag cttcatatat ttttccatga
     8881 gaattaaaca catccagtct ccacttctca cctgcaagcc aagctataac tctagcttct
     8941 attgcactaa aatctgacac tataaatcta tgaccttcac ttggtataaa agctgtcctt
     9001 atcaattgac ttaagacatc tggtacacta tcatataaaa gctctattaa atcaaaatct
     9061 ccttctttta ataggtttct agctaggtct aaatcctcta tatgattttg tggtaaattc
     9121 tgtacttgta ctaatcttcc tgcccatcta ccagtcctat tagccccata aaactgtagt
     9181 agacctctta ctctattgtc attaccttta gctaatttca tagcctcata tttctttata
     9241 gaagttttgg acattaattt tctaagttct aaaattctaa ctacattttc atcatcaact
     9301 tgttttaata tttctggaat actttctttt gttaggcttg taatctcaaa gccaacttta
     9361 tcacttaacc atttttttaa ttgagctgga ctatttggat tatttagacc agttatttta
     9421 attgcttctt ttgtaagttt ttcagtatat cttttatcac attctattgc attctctatt
     9481 aactctgtat ccactttaat accagtatca ttaattctct ggtctaaata ccataattta
     9541 atttctcttt cggtagtctt atacttacta agtttgtttc ttatttctct ttcaactaca
     9601 acatcttgtt tacaatattc tttaaattta ttccattttt ccatatcatg tattggtaaa
     9661 tttctagttc ttcccttgtt aacttttgta gccttacaag gtttacagaa atattgtatt
     9721 aatgctttac cttctttcat tttctgttta tcttcattaa actttaaagc cttagacaca
     9781 ctatctaaac ttcctggaag tcctagtgtt aacgccttta tcattgtaca tgaccactca
     9841 tttggtttta aattaatatt taaaaattta cttattgctg ttctttcaaa attagcatta
     9901 aatgctgatt ttataacttt attatcattt aaagcttcta ttacttcttt tggtaactct
     9961 tcatcattta ctaaatcaat aacttttacc tcttcattat caaaagcata ggcaaataac
    10021 agtatctcaa aattagcaga gtctacatat ctgtagactc caactttttt tatatctaaa
    10081 tcactatatg tttctatatc aattgataag gtcctcatta acttaagaag tcctcttctt
    10141 catcttcata ttcaaagtca tctgagaaat ctgcttctgc actagctcta gctcctccaa
    10201 gtacttctcc atctgctaac ttttgaacat tttgtaagcc acaacctatt cctttatttc
    10261 ctgcactatt atatgggaaa aagtttatac taactcttcc ataacaacca ctatatacct
    10321 ctgtattatc taatatttca tttaagtctt tatcaactat tcctggcttt tgagtactat
    10381 ttgcatttaa aaaatacatt cctacatatt cctctgcttc atcagctctt tctgcatctc
    10441 catcacgaag aggtgttttt aaattacctg gtaattttcc accccattta gaagttttac
    10501 cttgttcttt agctgcatca atagccttct ttattcttcc taaagtcacc ttatctgatt
    10561 ttggtattaa aatacaaact gaatacttgg gctctgcacc ttctaccatt gctctgcttt
    10621 taaagatatt gcaataactt aatcttacct ttcctgttac tacctttgtt gattgtactg
    10681 aattactcat aattttcttc ctcctaatat ttttatattt ggtatattga ttttatattt
    10741 gaagttattt ctccaaattt atctaaatct ctaacttgct tactaaacaa tttaattctt
    10801 ccacaaataa ctgttgaaga aacatttaat aactctccta tatcagatat ctttttatct
    10861 tgtaaaatcc aatctactat tttatcaata tcttttaaat atgactttct tgcaattttt
    10921 cttatataat caacatcata gttaaattga atttcataaa atccatgtat atcttttaat
    10981 ggtcttttac tatcttcaaa tacacttaca tctaaacctt ctatagagtt tctataatgt
    11041 ttttcgccat aacgcaaagc tttaaacatt tctattctta tgtaagaaac agccactgta
    11101 gaaaacttgc ctctgttact atcataattt aatgctgctt tatataaccc aatacatcct
    11161 tcttgataaa actcttcata taagtatgga tgttgctcta cataagactt aaaaaatcta
    11221 tttatactaa aatgtacaag attcaaatta ttttcaacta gtttagttat ttcttgatta
    11281 ctcataaaat tatatcccct tttaaatctt tttatagcta ctaggaatta aatattttta
    11341 aatatccttt gctcttactg atacaaagca tactggacta acttttatat gttctggtat
    11401 atacactcta tcaagatgat tgtcatttac ttcgaggaaa gttgacatca ctttatcctt
    11461 tattaaactt tctcttttaa aatccatcac tgtaactttt gcattttctt cagttgagtt
    11521 atattctgta atatttagtc tctttaacat atctagtaaa ttatcttttt cataatctaa
    11581 atctttttcg atactctttt ttctctcctt taaatctaaa attatactta tgctttcatc
    11641 taatttatct tttatacctg catctaatgg catgtaatat cccccattta tatttcaaaa
    11701 tcttttttcg ctgaatctat actattaatt tctgaccttt tatcactttc tactactaaa
    11761 gtagcttttc caactggttt tataattaaa tcacttagta gtttagcaaa actcttttta
    11821 cctatagctt tctccatatc actaattcct tttaaggtcc ttggcttata aattttttct
    11881 tcatcataat ctgaatttaa taaaacctta gcaacttctt gctcatctac atactttcta
    11941 ttacttctgc cttctacaag cttatatcct ggatatttaa caccatgctt ttcagcttgt
    12001 tctagtgcat aactttgaac atcctttaac caatcttgta tattcttagc aaaacctaaa
    12061 atatctgcta tctcatattt attaagagca aatgtgtcag caaaatcata ttttctagct
    12121 agtttaaggt tatccccagc tctttttctg caatcatttt tagctctaca aaatccacaa
    12181 tgactgccac ttacaaactc tccttcacca ttaaaagcca tttgagcctt cttcttaaca
    12241 ttatctgccc atgtaagtaa tttagtaact tctatttctt cacttgatat gttatcaagc
    12301 ctaggttgaa ttattgttgt tttaattaaa tcaatatcat atagcatttc aaactgatta
    12361 tatgccccta aaccatatag tctaagttga ggattctcta tagctgaaac ttctaaacct
    12421 tttccatact ttaggtctat aacttgaagt ataccatcag atattacaac aacgtctcct
    12481 gttccaaatc cttctggaac ccactcgcta aaatcgagtc tttcttctaa catcactatt
    12541 acatcatcac atatagcttt actatcattt actaattcaa ctacattctc aacataagat
    12601 tgtatatagt cctccatttc agagttataa tactcacttt tctgtatctt tttaattctt
    12661 gcattatatg ccttttcact tattttttca tactctagca ttaatttaac ttctgctaat
    12721 tcatgtgcaa ctgttccttc ttctgcatat atactagttg atggtggata gttttcttct
    12781 aattttatac taggagtgca gtgaagccat ctatgggctc cactcgcact aagtcttgca
    12841 tgttgtaatg gcatttatgt accccctata aactttctaa tttgttcata aaagctgaat
    12901 aatcttcttc ttttacttca cttaacttac tagctccaaa ttcaccaaat aactccttaa
    12961 gcttatcctt cttacctgcc ttacttactt gtgctgcctt agttcttacc tcttcttttg
    13021 tgtatttaac ttcactagtt gtattttcat tattcttttc tatttcttcc tttgcttctg
    13081 ctatttcttc tttaacgtct tcttttactt ctaccttttt aatcttttca gcttttttaa
    13141 cttcaacttt cttctcttct ttcatatctg ttatatttat aggttctatc tgcattgctt
    13201 ttcctagatt aagacctcct aaagcattcg ccactactaa tagtgcattt gtaaattctg
    13261 gtgcttctac tttaactttt acatttacat taacttcaac cattttaaat ctctcctttt
    13321 tgtgttatac tttacttgtg ttatatttta ttttttattt ttttgtgtgt tggttatttt
    13381 accaacactt ttttatttaa tattccaacc gatattttct taccagtttt aatatcttta
    13441 aatactatat ctgctataac ttttccatct tttttaagag ttactacatt cttattatta
    13501 gtatcaagac tcagcaattt catcccccct ctctactgct tttaaaagtt catccaaatt
    13561 tttaccttga tttctttcaa taaaatcatc aacttcatat cttgaaattt ttctaccatc
    13621 acctctaact agtgatttta tcaaacctgt gcttactaac ctacgcataa aagctgtatc
    13681 taactttaaa attccccttg cttcttctac tgttattaga taatttggat aacctctttt
    13741 tattaaaaca actatatctt taggctccag tacctttacc ttttgttcaa tagtttgact
    13801 cttaactcta tctgtctctt gtttacttat ctctatttct attaagcttt ttaagctatc
    13861 actaaatctt ttaactattt catttgtgtt atccattttt taatcccctc attattatca
    13921 aaatattctg ttttttattt cccaactaat tcatctaagg taacatctaa atagcccgct
    13981 atttttatta atgtgcatat agttggattt ttaatatatc taaaagttcc aaatttttct
    14041 ctataaagta gatactttta tttttgtggt gttttttgta tgggtttatt aagctctatc
    14101 ttaaaaacat cacaatcttt tatagctcta taaccgtttt cattcaataa atctactaat
    14161 gttgacttcc ctcttggtaa ttgtacaccc tcaacacata taatcatatt tgcatcaagc
    14221 gcatccatta aatgttttat ctctctttta cttaataaag gttttaagaa ttcttttact
    14281 tctttttttt cttttactca tgttttgtcc tccctatttt ctctaattta aaattaaatt
    14341 aattgtattt aatctttact agtaaaatac ttttattatt catgtacttc ataaaatagt
    14401 gtgtttaaca tatatagcaa aggactattt ttatctataa ctaattcttc ttcatcttta
    14461 atgtaaaatt ttattaagtc ttcatctata caataagtta tataattatc ttcgcccatg
    14521 cacataccta cttctttttc tatcttattt tggtctgcta tttttatatc tgctattgta
    14581 tcaagagtat ctactatttt ataaaatatc ctttctctag cactcaattc gttactttct
    14641 tcttttatat aagtaagata ttttattaat tcatcttctt ctatatcacc aaagaatttt
    14701 aacataaact taaaagtaaa atcatcaaag aatttttcat ccatctctaa gtgttcaaaa
    14761 ttcattttct tataatgcca tgctactttt gatgcaatct tatccaatat gtttttatta
    14821 tcttctatat ctttatcact aagtgaaatt ccacaatcaa tacctatttt attgttaatg
    14881 ctattcataa ttttcttaaa attatttacc atttctaatt ccttatctgt taagtctatc
    14941 tctccatcaa ctccaatact cactattcta ttattttctt tcattttctt attcctccta
    15001 tattgtttaa ttttattaat aatattaatt agttttttcc ataaaaactc catatttaat
    15061 agccatatct tttattacaa ttacataacc ttctattaaa taattttgtt cttgtataac
    15121 atccaggtaa ttaaattttt cccttctaga cttgcataca cctttttctg ccattttact
    15181 tcttctatta tttaatcttc gcttcaagtc aacattaaat cttttacaaa gaagttcata
    15241 actttctttt ctaagagtat ttatatattc atttccacct aaattttgtg ccattgatac
    15301 tatcaacttg cgtgtttcct ctctccaatc agttgtattc aatgtaacaa catcttttat
    15361 gctttttatt tcttgtttag tttctgttat atcttgtttt atttgttttt gctctatttc
    15421 ttgttttgct acagtatcaa atatttgctt aaacagttgt agttctggac ttaattgatt
    15481 tgtaattaaa gtatctttct ttttgttaaa gtaagactct tctaagttat caaactgctc
    15541 ccaagctttg tcagtatcca atattttgca gtgtctgttt gctcctcttt cagtccaaag
    15601 atacattttt gaagtaaatt ttaggttttc atattctgta tgaatacctt taaaattctt
    15661 taaatcatca ccttgtaata aaaaataatg tttaccttca ataaatctat ctttgttatt
    15721 gttaaaattg ttgcttatat ttcttacatc tgtttcatat acatctgcta gttgctgtgt
    15781 agttaaaact ctttcgttat ttctttctat tacttgtaag ttattcatgt ttgttacccc
    15841 ctatattttt ctttaacttc atcttcaatt tcaaaaagat attcagtact tagattcggg
    15901 aaaaacatta attgtatatt ttttgcttct atgtacttta acctgttagc atttcttaat
    15961 tttgcagaca ctgtacttac atttagattc aatgcttttg ctaatcctcc aactttgtta
    16021 gctgaaattc ctaacatctt acctatatct gtagctgaat aagtttttct ttccatttta
    16081 ggaagtggta ttaatgtttc tccagaaagt aattcaatag tctttgagta cataagctgt
    16141 ttatattctt ttatatctac tttatcagct aactctaaat aaatctttgc ttctctcgct
    16201 cttgcatttt ttaatcttgt ttgagcattc atgtatttta tttctgagtc atctttacta
    16261 cattcagatt ttttactact atatgtacct gttttacgaa tagatggtaa tacatctctt
    16321 gttacccaat gtttaaattt tttagcagtt aacaacttag aacttaatat taaagagtaa
    16381 agaccacttt cattaattat tttcatattt tgattacccc caggagtcgg tatttcacct
    16441 acccctttat cttcatcatc aacatgtctt ttcaaagcat cagatgtatc tttatatcct
    16501 aatgtctcgg ccacatcttt accaacaaac caaggctctc catttaaatc tataaccctt
    16561 atttctccaa aatcactgtt tttaaatact tgtaaattat tactcataat ttttccccct
    16621 agttttttgt attttctctc ccaacttcaa ataaatttga tagtttactt gtaaaatctt
    16681 ttggagattt tgtattcatt aaagtttgta ctacaacaca agaatctact gctgttataa
    16741 ttaaatctaa ttcactctca gtcaaagttt gaagttttgg aattgtatct aatatttttt
    16801 tctctctcat attatccacc gccttccttg ttctgtaaca ccatattatc acccttttac
    16861 ttgttagtca acactatttt aaaaattcgt ttattttttc ttgttgacta acaagattct
    16921 taaaattata atactcttag gggaggtgat ataattgtct aatgaaacta taagcacaag
    16981 aattagaaag ataagaaaag atgctaattt atctcaacca gcttttgggg aaaaactagg
    17041 tgttagtaaa gatgttataa gtaatataga atataataga gttgaaccaa aacctttgtt
    17101 tatcaattat atgtgtgatg tattcaatgt taataaagag tggcttttaa atggtgtagg
    17161 taatatgtat atatctacag aagatgatat tcttttaggt gaagcttttg cttatataac
    17221 tacatgtgaa aatgagaaac taaaaaaaat agtaattaat ctttgcaaac tagaggatat
    17281 atatgtggat gcaatatgta caactgtaga tggtataatt tcagatagaa aataacacat
    17341 taaatgataa aatgatatgc atttaaatta tattaattaa aatataaatc caaataaaac
    17401 aagtgctcat tatgaacact tgttttttat gttttttata taacttttta ttgtattttt
    17461 atattttata ttatgtatgt cctgtatgtc gttaattatc tctaagtcac ttttattact
    17521 atcatttaaa atccatttta taatattaaa tttttctcta ctacaatctt cctccagtat
    17581 tataactctt tctacttcat ccattagtac attcctcctc ataaatattt tatattaccc
    17641 caagtaatat tactttttat gctataatac atcatagaaa gcaacatatt ttatgattat
    17701 aattaattag tctcctttaa taggtcactt atcaaattat tttaatatat gttgaaataa
    17761 tttgataaat agtttaaaat aatgttatta tatactcaca taattttcat tcctataaga
    17821 ataataatgt tccaaattat ggaagtatat agtaatataa taacatgaac ttaataagat
    17881 gttcaataca taagcttaat gtttatgttt tatattaact atgtggtatg taaaaataag
    17941 tataattatt ttacaaatgt aacaagaaac attattatat taactatgtg gtatatagat
    18001 ttagaagaga taaaattctc ttctttttct atttacacaa tttttcaggc ttttgataat
    18061 atctttaaat ttacttaaat acctcctccc ttgtttattt a
//
LOCUS       DAKBNN010000067        18043 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000067 DAKBNN010000000
VERSION     DAKBNN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.067"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            109..1482
                     /locus_tag="OUB02_001910"
     CDS             109..1482
                     /locus_tag="OUB02_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885669.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1738..2805
                     /locus_tag="OUB02_001911"
     CDS             1738..2805
                     /locus_tag="OUB02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HCT8885670.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2949..3257
                     /locus_tag="OUB02_001912"
     CDS             2949..3257
                     /locus_tag="OUB02_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HCT8885671.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3250..4680
                     /locus_tag="OUB02_001913"
     CDS             3250..4680
                     /locus_tag="OUB02_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HCT8885672.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4758..5132
                     /locus_tag="OUB02_001914"
     CDS             4758..5132
                     /locus_tag="OUB02_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HCT8885673.1"
                     /translation="MKKREDDFEVRRKHLQELSEDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5126..7330
                     /locus_tag="OUB02_001915"
     CDS             5126..7330
                     /locus_tag="OUB02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HCT8885674.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7330..8739
                     /locus_tag="OUB02_001916"
     CDS             7330..8739
                     /locus_tag="OUB02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HCT8885675.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8724..9788
                     /locus_tag="OUB02_001917"
     CDS             8724..9788
                     /locus_tag="OUB02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HCT8885676.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9818..11239
                     /gene="nhaC"
                     /locus_tag="OUB02_001918"
     CDS             9818..11239
                     /gene="nhaC"
                     /locus_tag="OUB02_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCT8885677.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11503..11967
                     /locus_tag="OUB02_001919"
     CDS             11503..11967
                     /locus_tag="OUB02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCT8885678.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12265..12627
                     /locus_tag="OUB02_001920"
     CDS             12265..12627
                     /locus_tag="OUB02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT8885679.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            12695..13309
                     /locus_tag="OUB02_001921"
     CDS             12695..13309
                     /locus_tag="OUB02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT8885680.1"
                     /translation="MNEKWLEWAIELQSIAQAGITYGKDIYDIERYERIREISAEMIA
                     YKTDISTEKVRNLFCNESGYQTPKLDTRAVIFENGKILLVKENTGKWSLPGGWVEVNL
                     SVKENTIKEVKEEAGLDITADRIIAIQDRAKHNLPIYAYGVCKIFVLCTVIGGTFKEN
                     IETTEFSYFSENELPELATEKNTEEQIKMCFEACRTDTWTVIFD"
     gene            13329..13953
                     /locus_tag="OUB02_001922"
                     /pseudo
     CDS             13329..13953
                     /locus_tag="OUB02_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860815.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            14564..15424
                     /locus_tag="OUB02_001923"
     CDS             14564..15424
                     /locus_tag="OUB02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885681.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            15682..17796
                     /locus_tag="OUB02_001924"
     CDS             15682..17796
                     /locus_tag="OUB02_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8885682.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMVEKKTLEWSDVYPFIYIHATYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
BASE COUNT         7016 a         2132 c         3427 g         5468 t
ORIGIN      
        1 aatatatgat aagataaata caatagcaac attttattaa ttttttgggt ttttatatat
       61 ttaggtactg gggtgatata gttgaaccaa gagatatttt ttaaaataat gaatgaaata
      121 ttggaaaata ttgatgaagg ggtacatttt gttaataaag acgggaaaac tataatctac
      181 aataattctg tacaaaaaat ggaaaaaatg aacagacatg atattttaac caattcattt
      241 tttgatatag tcaacaaaat gaaaataaac aggagtacac tattagaagt tttggaaaaa
      301 agggaagtta tcaaagacaa tattcaaaaa tatctggata aaaatggaaa agagataaca
      361 gcaattaata ctacaatacc aattgatatt aaaggcgagt ttgtaggggc acttgaaata
      421 tcaaagaata tgacaacgat agaaaattta ctaaacagaa attataaaaa aaatttagtc
      481 aataaagtta gtagtagaca aaaaaagggt tatgaatttg atgatatttt aggggaaagt
      541 aatttgatta aagaagcaat agctaaatct aagaaagctt gcaagtctga tgcttcagta
      601 tttatatacg gtgaaactgg aacgggtaaa gagttattta gccagtccat tcactatgga
      661 agtaatagga aaaactatcc atttatagca caaaattgtg cagctttgcc ggaatcacta
      721 ttcgaaggta ttctttttgg aactagcaag ggaggattta caggagcaat agataggcca
      781 ggcttatttg aacaagctaa caaagggaca ttgttattgg atgagataaa ttccatgcct
      841 attatgttac aggctaaact attaagggtt ttacaagaag gttatgtaag aagggttgga
      901 ggaactaagg atatacctat agatgtaaga attattgcaa cgacaaatga aagtccaaag
      961 gtaattttaa acgagcataa aatgcgaaaa gatttatatt ataggcttaa cataattcat
     1021 ataaaaattc ctcctcttag agatagaaaa gaggacatac ctctattgtg caaaaaattt
     1081 atttcaaagt ataataaaaa gttaaataaa aaagtggtag gaatttcaga tgaagctatt
     1141 aaaattttaa aaaattacga ttggcaaggt aacataagag agctagaaaa cataatttat
     1201 tgtactatga gtatgatgga agatgaggta caaatagaaa caagtatgat taatctaaac
     1261 gactattaca atcttaaaga gaataaagag agtaacaatt attctttaaa cgatttggag
     1321 gacaaaacac ttagtaagat tatcagtgag atagaagaaa agtatatata tgaatcatta
     1381 gaaaaggcat cttacaatat aactaaagct gcatcaattt taggtatgaa taggcaaaat
     1441 ttacagtata aaataaaaaa atataatatc aaaatattat aaataagcaa gataaaaaaa
     1501 caaaatcaaa gtaaaataaa aaagcaaaat agaggagcaa aattattata aaagagcaaa
     1561 atattttgct ctttttttta tttctcataa gaaatattat tttttattta tgaaaataca
     1621 taataaaata attatagttt caacgttcca ataaatattt actttaattt taaagttggc
     1681 acgatttatg ctttaactaa ttattgagaa tatataaatt tttaggaggg tgtatatatg
     1741 agaaaagtaa gagtaggaat atggggattt ggagctatgg gaataggtat ggctaatatg
     1801 attctaaaga aagaaggtat agaaatagta tctgtttgta gtagaagtac ttcagggaaa
     1861 agtatgtatg atgttttagg aattgaaaga ggagaaagac cagaagttat tatcaataaa
     1921 aattatgaag aagtttttag agaaaaaagt gtagatgttg ttttgttagc aactgattca
     1981 tttactaaaa aggcttttga taaaataatt ttcttattaa atagaaaaat aaatgttata
     2041 tcaacagcag aacaaatggc atatccacaa gctgatgatg cagatttagc taaaaaaatg
     2101 gatgaagttg caaaggaaaa tggagtaagt atacttggaa caggaataaa tccaggattt
     2161 gtattagatt tactagtatt ggcactttca ggtacttgtg aagaagttac tagtataaaa
     2221 gctaagagag taaatgattt atctccattt ggaaagtctg ttatggtgga acaaggtgtt
     2281 ggagtaacaa gggaagaatt tataaaaggt gtagaagata agactatagc aggtcatgta
     2341 ggttttgtag aatcaataaa tatgatagct gatggattag gttggaaatt agataagata
     2401 gaacaaacaa aagagccaat aatgactaca gtagatagaa aatctaagta cggagaggca
     2461 ttggctggaa atgttgctgg ttgtagacaa tgtggatatg gatatgtaaa tggagaagtt
     2521 ttaattgaaa tggagcatcc acaacagata ataccagaag cagaaggtat taaaacagga
     2581 gactatgtat caataaaagg tataccaaac attgatttac aaataaatcc tgaaatacct
     2641 ggaggagtag gtacttatgc aatgatagta aactctattc cattaataat aaatgctaga
     2701 ccaggtctta aaactatgtt ggatatacca gtacctagag caataatggg agatataaga
     2761 aatcagatag aagttgaatt ggaagaagaa tcaaaggcta attagtaaga taacagctaa
     2821 ctaattagta agatagcaac tagctaatta gtaagataac agttagctaa ttggtaaaat
     2881 aatgtttaat ttaaaataac agtaaattaa ttagtaaggc aatagtcaat taataagggg
     2941 atgaaaaaat ggatgctaaa attaatgatt gggttattat acacaatata gtattgactc
     3001 cagaagaaag agctccacaa gttccagagg atactaagaa agtatcctta gaaatgtggg
     3061 ttaaaggttt tatacaaagt gatgcttcta taggcgactt agtagaagtt aaaacaatta
     3121 caggtagact tgtaaaaggt aatttactta aagtaaatcc atattatact catgattatg
     3181 gtaaatgtat accagagtta ctccaaatag gaatccaagc taaagaaata ttatttggag
     3241 gtgtatataa tgaataattc aacatcaatg aaagatatga gttatcaagc tgttatggga
     3301 agaaataacg aaataatgaa aaatgcaatc ggattggatt attcttcttt tgaacaagag
     3361 ggaatagggt ttgattatga gaaaatgatg agtgaaacag gatatacatt acaagatatt
     3421 gaagcaattc aatcacagta tgctgttgga aatactcctt taatagagct taaaaatttg
     3481 actaaattag ctagaaaatg tgctaaagaa ggtaaaggag ctagaatatt tgtaaaagat
     3541 gaagcaatga atgcatctgg tagttttaaa gcaagaagag cagctactgc agtatatcat
     3601 gccaaacaaa tgggatataa aggtgtaata gcagctacta gtggtaatta tggtgcagct
     3661 gtagcatcac aagcggcaat gcagggatta aaatgtataa tagttcaaga atgttatgat
     3721 tctaatggtg taggtcaacc tgaaatcata gaaaaagcgc gaaaatgtga ggcatatgga
     3781 gcagaagttg ttcagttaac tgttggacca gagttatttt atacgttctt agttttatta
     3841 gaagaaacag gctattttaa cgcatcatta tattcaccat ttggaattgc aggtgtagaa
     3901 actttaggtt atgagttagc tatccaattt agagaaaagt ataaaaaaga ccctgatata
     3961 gttgtttgta caaatgcagg tggtggtaat cttactggaa ctgcaagagg attgataaaa
     4021 gctggttcta ttaatactaa agtagtagga gcaagtgtag atttaaaagg attacacatg
     4081 gctagtgata atcaatttaa taaaaaatca tttacaacag gacatacagg ttttgggata
     4141 ccatactgta catggcctga taggtcagat gtacctaggt ctgcagctag accacttaga
     4201 tatatggata gatatgtatt agtaaaacaa ggagaagttt tttatacaac tgaattattg
     4261 gcacagctag aaggtataga aagaggtcca gcaggaaata catctttagc agcagcattc
     4321 tctttagctc aagagctaga tgaggataaa gctatagtgg tacaagaaac agaatatacg
     4381 ggtgcaggta aacatatatg tccacaactt acctttgcaa gagaaaatgg aatagatata
     4441 aaatttggaa atccaagaga agaaattgca ggagtaaact taatattacc agaaagacca
     4501 gaattattga aatgtgttga tattgatatg aataaaataa gaaagtcatt tataaaaaat
     4561 tgtatatcta ataatcacat tgataatatt gataagctaa gtaatcgaga tatagaattt
     4621 ttaatggaag aagtaaaaag ttcaagagat tttgttgttg atgtattaaa taatttatag
     4681 taattattgt tttttaattt aatataaaat aaaaattcag aaaattcgat taaatcaata
     4741 cgaatggagg aatgttcatg aaaaaaagag aggatgattt tgaagttaga agaaaacatc
     4801 ttcaagaact gagtgaggat gagttaaaag aaaggttttg gagtttagct acccagatag
     4861 tagaaccaat gatagaactt ggtaaaaaga atacaacacc atcaatagaa aggtctgtct
     4921 tgttgagaat gggattttct tctcttgaag taaaacctat tttagaaggg gttatggaaa
     4981 gaggtcttat aggtaaagga gcagggcatg tagtttataa attggctaaa tctaaaaata
     5041 taacggttag agaagcaggt ttgttattag ttaagggtga atattgggat gaagttacat
     5101 gtttatttag agaaggggta gaatcatgtt aaaggaaaat aaaaaattgg atatagacta
     5161 tatattaaaa gatttggata aatataaacc aaaacgaaga ggttgggttt ggagagaaca
     5221 cttggaaaat cttgaaatgg gaccttttaa atataaagac tgtacaaaac ctttaaagaa
     5281 gagtgtaggg ctaccatcat ctaagtattt caataatata gaccctcaac caagtccagt
     5341 tattactact gaaatagctt ctggtagatt tgaggatgat ataagaagaa tgagaatggc
     5401 agctcatcat ggagctgacc atctaatggt tatcagaact gctggacaat cacattttga
     5461 tgggcttata gaaggaactc cacaaggtat aggtggagtt ccaataacaa gaaaacaagt
     5521 aagagctcaa aggaaggctc ttgattttat agaagaagaa gttggtagac ctattaatta
     5581 ccattcatat gtaagtggtg ttgctggtcc tgaagtagca gtaatgtttg ctgaggaagg
     5641 agtcaatgga gcacatcaag acccacaata taatgttctg tataggaata taaacatggt
     5701 aaggtctttt gtagatgctt gtgaggcaaa gaaaataatg gcttttgcca atatagctca
     5761 aattgatgga gcacataatg ctaatgctac tgctagagaa gcatggaagg taatgcctga
     5821 acttatggtt caacacgctt taaactctat attctcagaa aaaataggta tagataaaag
     5881 taatatatgc ttatcaacag ttccaccaac agcacctcca gcaccatgtt taaaaataga
     5941 cttaccttac gctgttgcac ttagagagtt atttagtgat tataagatga gagctcaaat
     6001 gaatactaaa tatatggaat catcaactag agaagcaaca gttactcatg tattaaactt
     6061 attaacatca gtattaacta gagctgacat acaatcaact ataacacctg atgaaggtag
     6121 aaatgtacct tggcatatat acaatataga agcatgtgat acagcaaagc aagctttagt
     6181 tggtatggat gggcttatgg atatgataga acttaaagat gtcggtgaat taagagacaa
     6241 agcaagagaa cttaaagaaa gagctgtatt atatatggaa gaaatatctg aagttggtgg
     6301 atattttgaa tctgtggaac aaggattttt tgttgattct ggaaattatc cagaaagaaa
     6361 tggtgatgga atatctagaa aaataaaagg tggagttggt gaaggtactg tttatgaaag
     6421 agaagaagat tatttagcac ctgtaactgc tcactttgga tacaataatg tagctcaata
     6481 tgatgaaaat gctatagata atccatcaat gttaatagat ggatgtacat ttgaaaatcc
     6541 agacaaaata atatatattg atgagcttga cgattttgat aatgtagaaa atagactaaa
     6601 agaatcatat gagtacagaa atggaactaa aattaagcct gaaatggaat ggtgtgcaga
     6661 tggaattgta atgataacaa tgatgcttcc tactgataaa agaacagcag agtttgcagc
     6721 tttggaattt gtcaaaaaaa tgaacttaca agaaattgag gtcataagta gagaagttat
     6781 gcatgaatct gaagggacta gaatagaagt gaaaggaaga gttccgtttg atatagattt
     6841 aaacaattta gtaataccag aagaacctaa ggtactaact gatgaagaaa taagagcaga
     6901 tatagaagaa aaacctatga aaatagtttc agctacggtt ggagaagatg aacattcagt
     6961 tggtttaaga gaaataatag atataaaaca tggaggtatt gaaaaatatg gcatagaatg
     7021 ccactatcta gggacttctg tacctgtaga aaaattggta gatgcagcga ttgaactgaa
     7081 tgcagatgca atacttgcat ctacaataat aagtcatgat gatattcact ataaaaacat
     7141 gaaaaaatta cacgattatt gtgtagaaaa aggtataaga gataaagtaa tgattgcgtg
     7201 tggaggaaca caagtaacac ctgaaatagc tgttgaacaa ggcatagatg ctggatttgg
     7261 aagaaacagc aaaggcatac atgtagccac tttcttagtt gaaaaaagaa gagaaatgag
     7321 taataaataa tggaaaatag caaagtttct ttaaaaatag atgtgttggt agctgaaatt
     7381 ggaagtacta ctacagtggt aaacgctttc cacgatatta atactaacaa tcctatattt
     7441 ttgggtcaag gtcaagctcc aacaacagtg tttgaaggtg gcgatgtaag aaatggctta
     7501 tcaggtgcta ttaaagattt agctaataaa ttatctgtag atgatataga gtataatgat
     7561 atgtttgcaa cttctagtgc agctggtgga ttaaaaatga ctgttcatgg attagtctat
     7621 gatatgacag taaaagcagc taaagaagca gctttagggg ctggagctgt tattcgtcaa
     7681 ataacatcag gtagaattaa aagaactgat ttaaataaaa taaaagaaat aaacccaaac
     7741 ataattttaa tagctggtgg agtcgattat ggagaaagag atacagctat ttataatgca
     7801 gaaatgatag cttctatgaa tctaggcata ccagttatat atgctggtaa tgttgaaaat
     7861 caagaggaaa taaggttaat atttgaagat actaattata agctttatat aactgaaaat
     7921 gtatacccta aaatagattt attgaatata gaacctacaa gaagaatcat tcaaagtgta
     7981 tttgaagaac acataactac agcaccagga atgaaataca ttaaagaaat ggttaatgaa
     8041 aatattactc caaccccagg agctgtaatg gaagcttcta aacttttata taaaaatatt
     8101 ggagatttat taactctgga tgtaggtgga gcaactactg atgtacattc tgttacagat
     8161 ggaagtgact atattaataa aattttggtt aatcctgaac ctactgcaaa gagaagtgtt
     8221 gagggggatt taggtgtata tgtaaatatg aagaatatag tagaagttat aggtaaagaa
     8281 aacttacaaa gtgaactatc tatagatatt gatgcagtaa tagagaatta tcctccaata
     8341 ccaaaatcaa aagaagaaat attatttgtt gagaggttga caaaagaagc tgttgttaaa
     8401 gctatgctaa gacattcagg aaaaataaga aatatctatg gaacaactgg aaaagtcaaa
     8461 attgcagaag ggaaagattt aacagaagta agatatatag ttggtacagg gggagcactt
     8521 actaggctcc ctagtagaat tgaaatatta gataatatgc tcaaatacaa taaaaataat
     8581 gaattactat ttccaaagga aaaaacaaag atattaatag ataatgacta tattatggca
     8641 tctatgggtg ttttatctaa aaaatatgaa gaagcatctc ttaagctcct gttaaaaagt
     8701 ttgaattttg aggaggaaag attatgtatc ctaggttaga aatagatata gaaaagttaa
     8761 agcataatgc aaaattaatt tcgcagatgt gtcatgaaag aaatattaag atatcttttg
     8821 ttactaaatc attttgtgca caaaaagaaa tagtagaaga aatatgtagt gaaggaatag
     8881 accatatagc agattcaaga atacaaaact taaaaaattt acaagatata aatctaccta
     8941 agatattgat tagaatccca atgttaagtg aattagaaga agttatcaat tattgcgata
     9001 ttagttttaa ctcagaatta ggaacaataa agaaattaaa tgaattatgt gaaagtaaaa
     9061 atattattca taagatagtt cttatgtttg acttaggcga tttacgagaa ggatactttt
     9121 atgaagaaga cttttttaat aatgtaagag agatagtaag attaaaaaat attgaaataa
     9181 ttggtatagc tacaaactta acctgttatg gagctattat accatctagg gaaaatatag
     9241 gcagactggt aagtatagca aaaagaatgg aagaaaaatt tggaataaat ctagaaatag
     9301 tatctggagg taattcaagt tcgattcatc tgttgataaa taataatatg ccagaaggta
     9361 ttactaattt gagaataggc gagtccattc tacttggtag agaaacggca tacggtgaaa
     9421 atataaatgg aacatatcaa gatgcattta aattaatatg ccaagtagtt gaatgtaaag
     9481 aaaaaccatc agttcctatt ggagaaatag gagttgatgc ctttagaaat aaacctgttt
     9541 atgaagataa gggaatctta aaaagggcaa tcatagctat aggtaaacaa gatataaata
     9601 ttgactcttt aattccaata gacactgata taaaaatatt aggggcaagt tcagaccaca
     9661 tgatactaga tgtaagtaac acaaaatatg attataaatt aggtgacaat ttagaatttt
     9721 tattaactta tgggggtatc atgtcatcta gtacgagcaa atatgtttca aaaaaaatta
     9781 tagtgtagtg tttaaataat taggaggaaa ctattttatg aaagagcgag aatgtagaaa
     9841 agcaagtctg tttgatgcaa tgataccaat tacttgtttg atattatttt taagtctagg
     9901 agtattagtg tatggttcat cacctcatgt accactaata ggagcagcag cagtagcagg
     9961 acttgtggct gtatatagac ttggatttaa atggaaagag ttagaattaa gtatgtttaa
    10021 tagtataaag atggcaatgc aagcaatctt aatcataatt ataataggcg tacttatagg
    10081 aacttggata gtaagtggag tagttccatg tatgatttac tggggactaa aaattttatc
    10141 accaaatatt tttttagtag catcaacatt agtctgtgca atagtatctc ttgcgacagg
    10201 ttcatcttgg agcacaatgg gtacagtagg agtagctctt ttaggcattg gtcaaagttt
    10261 aggtatgcca attggattaa tcgtaggttc tataatatct ggagcttact ttggagataa
    10321 actatcacct ttatctgaaa cgactaactt agctccagca atggcaggaa cagatttgtt
    10381 tacacatata aaatatatgc tatattcgac tataccatca ttattaattt gtttagtaat
    10441 ttatggtgta attggaatga agtattcagg tcaagcacta gatataaaac aaatagaatt
    10501 aattaggtct acattagatt ctacatttaa tactttatca ccagtattat tattagctcc
    10561 agcaatagta attggattag ttgtatttaa agttcctgct ataccagggc ttatagtagg
    10621 tgctgtattg ggagtattgt ttgcagtagt ttttcaagga gaatctatga atacgatatt
    10681 agatgcagca caaaatggat atgtttcgtc aacacaaata caagaggtag atgctttatt
    10741 atctaaaggt gggattatga atatgatgtc cactgtatcc ttaacaatat gcgctttatc
    10801 tcttggaggt attttagaaa aaacaggtat gcttgaagta gtagcgtctt ctttactaag
    10861 attagcaaag ggtgtatttg gaacagtatt atgtactatg gtaacttgta caattaccaa
    10921 tataatagca ggtgaacaat atctatctat agttatacca ggaagaatgt ataataagga
    10981 atataaaaag agaggtatac atcctaagat gttgtcgaga gctttagaag atagtggaac
    11041 tttaacttct ccattagtac catggaatac atgcggggcg tatataacag caacattagg
    11101 tgtatctgca ctaacatatg gtccatacgc tttattaaat attataaatc caatagtatc
    11161 tttagtatta attgcatgta agtttaaaat agcaagaata gaagatgaac ctgaaacttg
    11221 tttaaatttt gatgtttgat attttaattg tttgtataaa ttttttatag aaaaagattt
    11281 cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt ataagagcac
    11341 tgtgtgacaa gaatgatgag attgtttttg ctatactttc gatataagaa atggaaggag
    11401 gttatgtttt atattcatat gaaataatta gttacacagt gaattttcgt ataatttggt
    11461 ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag aatatccaat
    11521 ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac aattgacttt
    11581 atggtagagt tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa atatctggag
    11641 gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat ggatttagca
    11701 aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa aaatgagatt
    11761 ctacaaagga ttgttcctgc acagaaaata ataactgcaa ttgatttagg accttatgaa
    11821 ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg atataaactt
    11881 catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag tgaatatctt
    11941 acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt agttgataat
    12001 tagaagaaca tttactgcta ctacataaat tttatcctaa ataatatcaa ggtgatttaa
    12061 tttatgtatt ctaatgtaac tgtagataat caatttataa ctaacttagt gtaatttaga
    12121 tttaatttat tattctaatg taaccccaat atattgttaa ggtctactta agatgctcaa
    12181 aaggctatgc ttaatttatt gttatataat ttgtagagtg gtataattta gaagtgatac
    12241 tatatagata tataggggga aaatatgaaa atatatgata tattaacacg tttttatatt
    12301 caagatatag aaaaagccat acctttttat gagaatcttt taaaagaaaa atgttctttg
    12361 cgtttttctt ataaagaagt tgggttagaa ttagcgcaaa taggaaatgt tttactatta
    12421 tctggttctg atgatgctct taaaccattt atagaaacaa aatctacttt tatggttgat
    12481 tctgttgatg aatggagaag ttatctattg gataatggag cagttgtagt aagagataag
    12541 aaaaaagttc ctacaggata taatatgaca ttgagacatc cagatggtac aattatagaa
    12601 tatgttcaac atacaaagca ggattagtta aaaatatatt ttactactaa gtaactaatt
    12661 ataatatatt cttcggaaaa ctggaggtaa ttttatgaac gaaaaatggt tggaatgggc
    12721 gatagaattg caaagcattg ctcaagccgg aataacttat gggaaagata tatacgacat
    12781 agagcgatat gagagaatac gagaaatttc cgccgaaatg atagcatata aaactgacat
    12841 atcaacagaa aaagtaagaa atcttttttg caatgaaagc ggataccaaa ctccaaaact
    12901 tgataccaga gccgttattt ttgaaaatgg aaaaatactt cttgttaaag aaaatacagg
    12961 gaagtggtcg cttccgggtg gttgggttga agtaaattta tcagtcaagg aaaatactat
    13021 taaagaggtc aaagaagaag ctggattaga tataaccgcc gacaggatta ttgcaattca
    13081 agaccgtgcg aaacacaatt tacctatata tgcttacgga gtatgcaaaa tatttgttct
    13141 ttgtaccgtt atcgggggta cattcaaaga aaatatagaa actacggagt tttcttattt
    13201 ctccgaaaat gaacttcctg aactcgccac agaaaaaaat acagaagaac agattaaaat
    13261 gtgctttgaa gcatgtcgta cagacacttg gacagtgatt ttcgattagg ttaattaagg
    13321 agaaattaat ggattacaaa gaaatcgtct ttgatgtaga cggcacattg attgatactg
    13381 aatacgccat tttacactct ctacaagaca cgataaaaca aatcaataga aagataattc
    13441 ctatcaaaga attgtctttt gctttaggaa taacaggcga ggacgcttta cgttatttga
    13501 aaatcgaaga tataacaatt gctctatcaa tgtgggatag gaatatggaa aagtataaga
    13561 atacagttct tgtgttcaaa gaaataaagg aaatgcttac tgaactcata aaattaaata
    13621 ttggcattgg aattgttaca tccaaaactc atgaagaatt tagaaacgat tttctgagct
    13681 ttgacatagc accatacttt aatactgtag tatgctccaa tgacactcta gagcataagc
    13741 caaaagcagt accattattg aaatacatgg aactgacaaa atgtcaaaac aatgaactgc
    13801 tttatattgg ggatagtata tatgcaatgt gcctatcgtg caaatgttga ttttggactg
    13861 gtaaaatggg gttctggcga tagagtaagc gctgtaatat cttttaccac accattggat
    13921 ttgataaggc aactatctgt atcaagcaac taacaatcat agaaaatagc aaagtcagcc
    13981 aagccagtca atggtcaaga tgaacggcgc atttcatgtg ccaccgttga cagcaccgtc
    14041 tgattttgct gacgggcaat caagatggga aagccctaaa atggcttccc gcctatttca
    14101 attttgagag gaaaatccgt ctgctttttc gtgagtgtgg ctacaagttg gagcgtagga
    14161 tgagggacgg gagctttcat atacacatgt atgctgctga aacccgccct gcgcttgtgg
    14221 cttcacgcta tgcaatcaca gtcctgtttt cctgccgctt caaatgccct tgccttccct
    14281 gacggcaacg gcatttttac ggcaacgcca acagagcgta taacacacta cactttgcaa
    14341 gtaaagtcgt ttgccaaggg gaaagcccct ttggaaattc cagacaacaa aacagtctga
    14401 acacctcgcg ctttccctgt gcttgatgtt tagcttttat ttgttgtaag cagccccgtt
    14461 tcgtggtctg cgtgtagttc cttgtaaact gacctaagat tcattaaata taactaagtt
    14521 ataattaata taatgttgca tggataaaag gagggaagct gcaatgtctt acaaagtaat
    14581 taatgtcata actgctattg actatataga ggagcattta tcagaaaagc tggatttaga
    14641 tatagttgct aatgctgttc attactcaaa atatcacctg catagaactt tcacaacttc
    14701 tgttggtcta acaatgcatg attacataaa gcggagaaag ttgacagaag cagcaaaatt
    14761 attggtattt tcaaagaaac caattattga aattgcattg atagctggat atgaaagtca
    14821 acaggcattc acttctatat ttaaagctat gtataaaaaa tctccaaata aatataggaa
    14881 agaacaagaa ttttatcctt tgcagctaag atttgttttg aaaaaagata acctattttc
    14941 aaagaatgta ctagagttgg aaaaagaaat taaacttgca agtatgtctg acataccatt
    15001 atggatgaat ttagtgagat tagtcataga tggatttcca aatttgcaag aggaagaata
    15061 tatatatcaa ttagaacagt atattttaga agaaagagct ttaatattaa aacttaataa
    15121 tgtcgctatt gcaaatatgg tgtttaatag agaaactaga agtattgatt tttttggaat
    15181 acatccacag tatcgtaatt cagacattgc acaagtgttt ttgaagaaag ttattgaaga
    15241 tttcttgata gatacagata tttcaattac tacctttcga gagggtgaca aagctgatac
    15301 aggacataga gatatgataa agaagttggg ctttgcagaa gcagaattgc ttgtggaatt
    15361 tggatatcct actcaaaaat ttattcttcc ttgtgtagaa gaaagagata atagacaaaa
    15421 ttaattgtag attaaatgaa aagtttacca catatttttt gtttgaatta ttttatatat
    15481 aagagcattt ctgcttttaa tgtattttgc ttgtaagtgt acttatggta ataaatatag
    15541 aaagatttag ttttttaaaa aaggaacttt aaagctaaaa agctaacatt taagttaaat
    15601 agcaagaatg attaaaaact ccaaaacaca attaagtata atgattcagt gtaagtactt
    15661 aattaacaaa ggaggattta aatgtctgaa aataaacaag tagtaaattg cgatattatt
    15721 gcatctaatt ctaaacataa tatgtgtgaa tatgatgcag agttattaac tttttttcca
    15781 gatgagattg cacatctaat agagataaat aacaagctag acaatgcttt taagaaagct
    15841 gaaaatcttg ttgacaagct tgataaggat tatatggacg ctaaaatgta tatggtaaaa
    15901 aatcgtggtg aaatagaccc acatgaaatg tttcaaaatg aacaaggact aaaacaaata
    15961 gataactatg gggcttttat ggtaaaagtt cgagataaga ttaataaaat caaagattct
    16021 ccatactttg ctagaataga ttttaggctg aaagatatgg atgatgaatc taagtattat
    16081 attggtagat ttgcatttga ttatgaagat gagcttataa tacttgactg gcgttctcca
    16141 attgccagta tgttttatga ttatgaaatc ggaaaagctg gatatgatgc tccaatagga
    16201 tgggtggatg gagaaataac aagaaaacga caatttaaaa ttaaaaatgg aaaattagaa
    16261 tatgcactag aaagttccat taatatacaa gatgatattc ttcaaaaaga acttagtcac
    16321 acttcagatg aaaaaatgaa gtcaatcatt tcaacaatac aaaaggaaca aaatcaaatt
    16381 attagaaatg ataaagctga tacactgatt attcaagggg tagctggttc tgggaaaaca
    16441 tctatagcat tacacagaat tgcttttctt ctttatcgat ttaaagataa aatatcagca
    16501 aataatgtaa ttatcctatc acctaataaa gtctttggag actatatttc caatgtcctt
    16561 cctgagcttg gagaagagcc attatgtgaa ttaagttttg aaaatatagc agaggtacaa
    16621 ttagatagag ttattaattt tgaaagtgag aaagacccat tagaaataaa tgatgctaaa
    16681 tggtctgaaa gagtgcgttt taaatctaca cttgattttg taaaacttat tgatgattat
    16741 ataaaacaaa tgccaaataa gatttttatt ccaaaagact acacttttgg aagttttaca
    16801 gctaaaagtg actggataca gagtagattt gaggcatata atagatatcc agttaagaag
    16861 agattagaga aggtagctga agacattcat tataaatttg aatcagataa tattatggaa
    16921 gaagatttac caaaggtaaa aagtatactt aaaagtttga atggaatgtt gacaataaaa
    16981 aatactctta cactatataa ggatttcttt aaacaaatga atatatcaaa catgtttgtg
    17041 atggtagaaa agaaaacact tgaatggtca gatgtatacc catttatata tattcatgct
    17101 acatatgaag gtatacaaga agataagatt ataaggcata ttgttattga tgaaatgcag
    17161 gattatacac ctattcaata tgctgtaata aacttacttt ttaagtgtaa aaagactatt
    17221 cttggagatt ttggacaatt ggttaatcct aatcatactc atacattaga tgatatgaaa
    17281 cagctatata atgatgggga attagttatg ttgaacaaga gttaccgttc tacttttgag
    17341 attattaatt tcgccaaaaa gattcaagac atatcttcat tagagcctat tgaacgacat
    17401 ggtgaagaac cagctttatt aaaatgtaat aacgagcaag atgaaataaa taagataaaa
    17461 atagaaattg aagaatttaa aaagagtgat aatgcgactc ttggtattat attaaagaca
    17521 gataatgacg ctaaagctgt atatgatgcg ttaagagaag aatatagtgt tcacttaata
    17581 tcttctgaaa gttctagctt tacaaaagga gtatccataa cttctattaa aatgtcaaaa
    17641 ggtttagagt ttgatgaagt tattgtacct tcagtaaata ataaaacata ttatagtgat
    17701 tatgaccgca gtttgttata tattgcctgt acacgagcta tgcataaact taagttgaca
    17761 tatacttgtg aattgactca acttatagat atgtagatta tatacttaat tatgtaagtg
    17821 tttacttata taaatataac ctgtcaataa aattcattaa taaatcaata aaaacaatat
    17881 ttgtaaaagt aaaagctata aattatatat taaattttat aatttatagc ttttataata
    17941 ctgcttatct taatcctccc aatagcaaaa taattttaaa catgccatat tttataaatg
    18001 taataacaat tatacaatta aaataacttt cccaaactaa ctt
//
LOCUS       DAKBNN010000068        17571 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000068 DAKBNN010000000
VERSION     DAKBNN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.068"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            141..3689
                     /locus_tag="OUB02_001925"
     CDS             141..3689
                     /locus_tag="OUB02_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8885683.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3874..5808
                     /locus_tag="OUB02_001926"
     CDS             3874..5808
                     /locus_tag="OUB02_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCT8885684.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5914..6909
                     /locus_tag="OUB02_001927"
     CDS             5914..6909
                     /locus_tag="OUB02_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8885685.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6881..7666
                     /locus_tag="OUB02_001928"
     CDS             6881..7666
                     /locus_tag="OUB02_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCT8885686.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7654..8352
                     /locus_tag="OUB02_001929"
     CDS             7654..8352
                     /locus_tag="OUB02_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885687.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8475..8948
                     /gene="ybaK"
                     /locus_tag="OUB02_001930"
     CDS             8475..8948
                     /gene="ybaK"
                     /locus_tag="OUB02_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCT8885688.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9145..9984
                     /locus_tag="OUB02_001931"
     CDS             9145..9984
                     /locus_tag="OUB02_001931"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HCT8885689.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            9997..10545
                     /locus_tag="OUB02_001932"
     CDS             9997..10545
                     /locus_tag="OUB02_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HCT8885690.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGDVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10611..11792
                     /locus_tag="OUB02_001933"
     CDS             10611..11792
                     /locus_tag="OUB02_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCT8885691.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12087..12473
                     /locus_tag="OUB02_001934"
     CDS             12087..12473
                     /locus_tag="OUB02_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8885692.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYNMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12623..12886
                     /locus_tag="OUB02_001935"
     CDS             12623..12886
                     /locus_tag="OUB02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8885693.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13015..13857)
                     /locus_tag="OUB02_001936"
     CDS             complement(13015..13857)
                     /locus_tag="OUB02_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCT8885694.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14152..14886
                     /locus_tag="OUB02_001937"
     CDS             14152..14886
                     /locus_tag="OUB02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8885695.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14896..16029
                     /gene="nagA"
                     /locus_tag="OUB02_001938"
     CDS             14896..16029
                     /gene="nagA"
                     /locus_tag="OUB02_001938"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCT8885696.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAKVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16128..16877
                     /gene="nagB"
                     /locus_tag="OUB02_001939"
     CDS             16128..16877
                     /gene="nagB"
                     /locus_tag="OUB02_001939"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCT8885697.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17052..17324
                     /locus_tag="OUB02_001940"
     CDS             17052..17324
                     /locus_tag="OUB02_001940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885698.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         7019 a         1860 c         3114 g         5578 t
ORIGIN      
        1 ttaagctatc ttatttaatt ctaatacagg aagaaggtaa atgtatattg aaaaaatcat
       61 agaaatagaa gtaaacaatt taaataaata caaaggtttg caaaagaagt aatataatta
      121 tatacaatga ggatgtgagt atgatgaaca gactaaagcc aagaatttta ggtattagta
      181 tcttgtttat tattataatt attttaacat gtggagctgt tttcaagttt aaatttaaat
      241 tagataataa attaaaagaa accacacata catatttgtc tgagtttaca gaacacaata
      301 taattaatct caagacaaga cttagtggtc aatttgacat gcttgaatct attgctaact
      361 tcataggaaa cttgaataat ataaatgatg aaatggtgat tgatttaatg gattctgatg
      421 taaagcgaag ttctttaatt agaatgacta tctctacatt agatggggaa tgttattcta
      481 atgatggaat tacccataat gttcaagata gagattattt taaaaaagct aaaaatggag
      541 aaaagaatat atcagagcct ttaaaatcta ttgttaataa taaagaaata atagttttat
      601 ctaccccagt ttataaaagt gggaaaatag ttggtgtttt aagtggaatt tatgattcaa
      661 aaaagttaaa tgagttatta ggattatctg catttgatga aaaagcagat gtttttgttg
      721 caaagaaaaa tggagatata gttgctcaaa aaacaaataa aaatagaaag acgctatcat
      781 gtatatttca gctttttgac aaagataact gtgatgaaga tgattgtcta aaaactataa
      841 aagaaaatat gaaaaatgat aagtctggat attttaatta taacttagat aatgaaaaat
      901 ttatgttaaa ctatcagcca cttggagtta atgattggta tgttttttct gttgtgccaa
      961 ataaaatagt atctattcag tcagagaata tatcttatga tgtatatatt tttgcattag
     1021 aaatactact agttatattg atttttataa tatatatagc atgtattata aataataaca
     1081 ccaaaattat tataaatgaa aaagaaaaat ttaaagtact cactgataat atattaggag
     1141 gggttaaaac ctgtttaaaa gatgaatata tgactatgac atatgtaagt caggggtttt
     1201 tagagcttac aggttactct tgtgaagatt taaataaaat atttaaaaat agatttaaag
     1261 acatgattta taaagatgat ttagacaagt ttaattgtat tattctaaat caattagaaa
     1321 aacaagataa aattgaagtt gaatatagaa ttgtaagaaa agatggaagt ataatttgga
     1381 ttttggagag aagtaagtct ataaatgata ataaaggaag tgagtgtatt cagggagttt
     1441 taactgatat aacagaactt aagaaaatac aagaagaatt agattcaaaa agatttgaaa
     1501 tacaggctat caatgatagt attgttggtg gaatagtgat aactgagata aatgatgatt
     1561 ttgatagtat atatttaaat gaaggttatt taaatatgat tggatataca cgcaaacaac
     1621 tggaagatga gctaaataat aaattaatca aacttatttt taaagatgat agaacagaat
     1681 taattaatag tataaacaaa caactcaaat taagtgatac atttgcatct gaacaaagag
     1741 ttgaaaaaag ggatggcact attatatgga ttttattaaa aggaagaaag atgatttatg
     1801 ataatggaaa atctgtactt tgttgtgtta ttgtagatat tacttctgct aaattattag
     1861 agcaagaact taaaaagagt gaagaacgtc atgaaatcat tttaaagcag acgcaaaata
     1921 ttataataga atgggatata gaaaaagata aaatgaatat atcacctaat tggtataata
     1981 agtttaaata tgaccctata actgaaaacg caacaaaaaa tataattaaa tcagtagata
     2041 tatatcatga tgatatggat aagtttattt cattaattaa acttacttca caaggtagtg
     2101 agtatgaaga gtgtgaaatt agacttagaa ctagagatac tatttacgct tggtttagta
     2161 taaaggcaac aactttattt gataataatg gtaaacctta taaagtagta ggaagtttga
     2221 ctgatattga tgaatcaaaa cgagaagctg aaaagttgaa agaaagggca gaaaaagacc
     2281 ctcttacaga tttgtataat aaaaaagcat cacaatcact tatagaagag tacataacat
     2341 tggataaaaa aagaaaaggt gctttaatga ttattgatat tgatgacttt aaaggaataa
     2401 atgataattt aggacattta tatggggatg ctgtattaag tgaaattgca aatgacctaa
     2461 taaatctatt tagagcaacc gatatagtag gaagaattgg tggagatgag tttattgtat
     2521 ttataaaaga tatttctgaa acaagagata ttataaataa agcagaagaa ttggtaaaaa
     2581 catttgaaag gtctttttta ggtaaaggac gaaattataa aatatcatta agtgtaggaa
     2641 ttgctaagtt tccacaagat ggtatggact atatgacact atttagaaat gcggatatgg
     2701 ctctttactc agccaaaggg aagggtaaga attgttatac attgtatgat aaaaaaattg
     2761 ataaagtcca atatataact aatacaagaa atgcaggaga tagtgataat gtagagatgt
     2821 cttttaaaga acatatcaca aattatatat ttgatatact atatgaatca agtgatatag
     2881 aagtggcagt aaatttaatt ctaagtatcg taggtaaaca ctttaatgtg agtcgcatgt
     2941 atatatttga aaattcagat gataatcgag tatgtaaaaa tacatttgaa tggtgtaacg
     3001 aaggtgtaac ctcggaaatt aataatctta aaaatatttg ttacaaggat ttaggcaact
     3061 atcttttaaa ttttaatgca gatgggttat tttattgtaa tgatattgaa aatttaggta
     3121 gtgatgtgaa aaaagtactt aagccacaag gcatacgttc aatattacaa tgtaaaattt
     3181 tagagggtaa tgaatttaga gggttcatag gatttgatga atgttcatat aatcgtatgt
     3241 ggactaatga tgaagtgaac actttgacat ttatatctaa aacgctcagt atgtttttat
     3301 taaaaatgcg tattcagagt aagctagaag agtctttaac aattcaaaaa tctgtgatgg
     3361 ataatatgga tatatgggca tatgttgttg acaggaatac ctatgaatta ctatttatca
     3421 ataaaaaaat gcttgagtca gcaccaaaca ctcatgtagg tgatttatgt tataaagcga
     3481 tatggaataa tcaagataaa ccttgtaaaa aatgccctat gatatattta aaagatggtg
     3541 atgatactta taaatcattc atgcataata aaaagtttga ggtacaggca aatataacta
     3601 caactacttt gaaatggttt gatggtaaag atgcatgttt aatatttgga agtgaaataa
     3661 ctagtattga agctgaagaa aatacttaaa atactgtgtt atatatatac ttaaaatcgc
     3721 aatttgaatt tattagactt aaaatgtcta tataaatcat ttaagttgtg gttttaagta
     3781 ttttttatga tataattaag cgttaagaac aaaattaaaa gatacttttg tataaaaaaa
     3841 tatttgttaa atatatattg agcaggtgat aatatggaaa ataagttttt accaataagc
     3901 aaacaagata tgatagatag aggatgggaa gaattagatt ttgttctagt tacaggagat
     3961 gcttatgtgg accatcatag ttttggaaca gcaataatat ctagagtttt agaaagtgct
     4021 gggtataaag ttggaataat agctcaacca gactggagaa ctacagatga ttttatgaag
     4081 ctaggaaaac ctagacttgc atttctagtt aatgcaggaa atatggattc aatggtcaat
     4141 cattattctg taagtaaaaa gcatagagaa aaggatatgt attctccagg tgggaaaatg
     4201 ggttatagac cagatagagc tactatagta tattgcaata aaataagaga agcatacagt
     4261 gatgttgcaa tagtaatagg tggaattgaa gcgagcttaa gaagatttgc acactatgat
     4321 tattggagtg ataaggttag aaagtctatg ttaatagata gtggagcaga tttattagtt
     4381 tatggtatga gtgaaaagca gattgtagaa gtagccaatg cacttaatga tggatatgac
     4441 ccaaaataca ttagacatat tgatgggaca tgctatattt cagatacatt ggaagaaata
     4501 tatgataaat atatattaat tccatcatat aaagaaattt gtgaagataa aatgaagtat
     4561 gtagaagctt ttaaaattca atatgatgag caagacccat ttagaggtaa tataattgtt
     4621 caacctcatg gaagtaagta tctggttcaa aataagccag aaaaaccact tagtagagaa
     4681 gaactagatg aagtatatgg attaccatat cagaagacat accatcctgt gtatgaaaaa
     4741 tttggaggaa taccagctat agaagaagta aaatttagta tagtaagttc aagaggctgt
     4801 tttggtagtt gttcattttg tgcaataaca tttcatcaag gtagagcagt gcaaagtaga
     4861 agtgaaaaat caattattga tgaagcaata ggaataacaa atttagatga ttttaaaggg
     4921 tatatacatg atgttggagg tccaacagct aattttagaa gaccagcatg taaaaaacaa
     4981 attactaaag gtgcttgtaa aaatagacaa tgcttatctc catcaccatg taaaaattta
     5041 gatgcagacc acagtgaata cttacattta cttagagctg taagaaaatt acctaaaata
     5101 aaaaaggtgt ttgtgcgttc tggaattaga tacgattatg taatggcaga taagaacaat
     5161 aaatttctta gagagttgat agagcatcat gtaagtggtc agttaaaggt agccccagaa
     5221 catatatcag aagaagtttt aaagtatatg caaaaaccag caggaaaaac ttatgataaa
     5281 tttagacaga agttttttgc tataaatgaa gaattaggta aaaagcaata tttgataccc
     5341 tatttaatgt ctagtcaccc tggttctacc ttaaatagcg ctatagagtt agcagagtat
     5401 ttaagagata ctcactacca accagagcag gttcaggatt tttatccaac tccagggact
     5461 ctatctacaa ccatgtttta tacaggtatt gacccgctta caatgaagcc agtttatgtt
     5521 cctaaatcaa aacgtgacaa ggcgatgcaa agggctttat tacaatatag agcacctaga
     5581 aattatgatt tggtatactc tgcgttagtt gaagcaggaa gagaagactt aattggtttt
     5641 ggacatagat gtcttataaa gccaaaaaat gaaaagcctt attttaatag aaataattct
     5701 aaaaaaaatg tatctaaagg aacaaataaa aacaaaaaaa ctaatacaaa taacagaagt
     5761 aataaaaatc aacaaaaaag ttcaactaaa aaaaagaaaa aacattaaag tataaataaa
     5821 aatacttgaa aaagaaggat tattcgataa attaacagaa attagtataa ataaatataa
     5881 ttattttaat gacgttacct ttgggaggaa gagatgaaaa agtatatttc tataatatta
     5941 ttagtggtgt tgactatggt tgttttggta ggatgttcac caggaaaaga taaccctaaa
     6001 aataaggagc tatcagttgt caaacaagta aaagtagctg ttccagatgg actaccagca
     6061 gtggcaatag ctaaacttgc aaatgaaaat ccagagatta aagaaggata tgaaacagtg
     6121 tatagcattg aaaagacacc tgaagcgatt tccactagag tgatgaaaaa agatgcagat
     6181 atagctatag ttccttcaaa catggctgca atagcgtata ataaaacttc atcatataat
     6241 attgttggaa ctgtaggaat gggttcactt tatttggtgt caacagaaaa tataaaagat
     6301 tattctgatt tagttggtaa agaagttgga tgtactggaa aaggacttac tccagacata
     6361 actataaaaa gtcttttgtt acaaaaggat ataaattatt cagatataaa atttaattat
     6421 gtaaattcag caagtgaact ggtaccatta ttggctactg gtaaagttaa aacaggtatt
     6481 gttccagaac ctgcacttag tgctttgatg tctaaaaatc cagacataaa aattataaaa
     6541 agtttaaatg actcatggaa ggaagtaagt ggttcaaaag atggttatcc tcaatctact
     6601 ttaattgtaa agtctgaatt tttaagagat aataaagatt ttgttgattc ttttgtaggt
     6661 caattatcaa atagtataga ttgggcaaac aaaaatcctg aagaattagg tgcatattct
     6721 gaaaaaataa aaatatctac tgaatcaaag ataataggaa aatctcttga aagagctaat
     6781 ttaaaatata ttccagttaa ggacatgatt aaagattata aaaattacta tgagaaatta
     6841 gctaattttg atgataagac attaggaggc aaagtaccag atgaagcaat atactttgtt
     6901 gagaaataaa aaaaataaag aacaatttat agataaaatg gaggtaacac tttcttgtgt
     6961 tatcctctta tttttatggc aaattattgc tttaaaaatt gataatgata tttttttacc
     7021 aacagtaggt caagtattta atagtataaa agagattatg ctaaattcta gtttctatat
     7081 agatatactt tactctatgg ggagatgttt ttttagtttt ttactggcta tgatttttgc
     7141 tattatactt tcaatagtgt cttatttaaa tagatttttt agaaacttat taaagccaat
     7201 caatgctttg acacgttcaa taccaactat gatattggta gtacttgcac ttatttggtt
     7261 tgaaaaggat agtacccctt ttatagttgg atttactata gtttttccta tattgtatga
     7321 taacgttttg ggcgctatat tgaatataga caagaactta ttagaaatgg ctaatgtgta
     7381 taaaataagg tttatagata aggtgttaaa aatttattta ccagccataa aatttcaaat
     7441 tatatctata ctagtatcaa cattctcttt aagtttgaaa gttgctatag caggagaggt
     7501 atatagtcaa cctacatatg gtattggaac tatgatacag actgaaaaaa ttaactttaa
     7561 cacatctgga atatttgcat ggataattat tgttctttta atatctgcta ttttacaaat
     7621 agctcaaaag tttttagcaa ggagagcttt tttatggaaa agataaagtt gagtattgaa
     7681 aatataaata aaagatatga tagtcgtatt atatttagag attttaatat tgatttttat
     7741 gtaaatgagg taaattgtat ccttggaaaa tctggttgtg gaaaaactac actcttgaac
     7801 ataataagtg ggattattaa aaatgacacc aacaatctaa atatcaaaga aaatttaaat
     7861 agagtaggaa ataagcttga agctagttac atatttcaag atgatagact tatagactgg
     7921 ttaacagtag aagaaaatat aaagatagtt gtaaataaat actataataa aactcaatta
     7981 aataaaattt gtgatgaata cttggaattg gttggaatta gtgattataa aaaattctat
     8041 cctcagatgc ttagtggagg tattagacaa agagtaaata ttgctagagc ttttatatat
     8101 ccatcaaaaa atattataat ggatgaacct tttaaatcta ttgatgcaaa gaatacacag
     8161 cttataatgg ataatttcag aaatatatta agaaaagaaa aaagaactgt gttatttgta
     8221 actcataata ttgaggaagc attgttttta gcagatagga tatttatttt aggagactct
     8281 ccaattagaa taaaaaaaat attaaaaaat tctaaggaat tggaaaaaaa tgaagttttg
     8341 aagttgatat agagttataa taatactgtc gttttaaaat atatttggtt aaaatctata
     8401 agactatatt aaaatagtac agaatattca aaactgtgta taatgttcgt attttataaa
     8461 aaggaagtga atgtatggtt aagactaatg ctatgagaat attagattca aataagattg
     8521 attataaagt aatgtcttat gaagtgaaaa gtgaacatgt ggatggtgtt gaggtggcac
     8581 atgatatagg aagagatgta aatgaagtgt ataagacatt agtaactcaa ggagttagta
     8641 agaatatata tgtatatgta ataccagtac atgaaaactt agacttaaaa aaggctgcaa
     8701 aagtagctaa agagaaaagt gtagagatga ttcatgtaaa agatataaat aagcttacag
     8761 gttatattag aggcggatgt tctcctatag gtatgaaaaa gctttataaa acttttgtaa
     8821 atgaaagtgc aaagaattta gatacaatta ttgtgagtgc aggaaaaata ggatatcaaa
     8881 tagaactatc tccatttgat ttacaaagac ttataaaagt agaatttgta gatgtaataa
     8941 aaaaataaga attacatata aaatatagag cttatattct taataatagg tgttgtagta
     9001 ttaataaaat gaataataat tttaaaataa atattcagtt acttatgtgt aattggtata
     9061 aaaatattga gaaattttaa aaacaatggt aaagtgtaag tataatcacg aatatttttt
     9121 ataaagtaat ggagggatgt tcgcttgaga aaaataaaat ctgaacaaat cgtcgagcag
     9181 gtaaaaaaac tttgtataga agcgagccta tatttaggag aagatgtttt aagctgtata
     9241 aaagaaaaag cgaaaagtga aaagagtgaa gttggaaaaa atatattgaa tatattagtt
     9301 gaaaatgctg aaatagctaa agaaaaaaat ataccaatat gccaagatac tggtatggct
     9361 gtattttttg ttgaaatagg acaagaagtt ttaattgaag gagacacact tactgatgct
     9421 ataaatgagg gagtaagaca agggtatgaa gaaggctact taagaaaatc tgttgtaagt
     9481 ccaattaaca gagtaaatac aaaagataat acacctgctg ttattcatta tgatatggta
     9541 aaaggtgata aaattaaaat agaatttgca gctaagggat ttggaagcga aaatatgagt
     9601 aagatgaaaa tgttaaagcc atcagatgga ttggaaggaa ttaaaaaatt cataatagat
     9661 acagtttcag aagctggacc taacccatgc cctcctatgg taattggagt tggtataggt
     9721 gggactgtgg acaaatgtgc tcaaatagct aaaaaggcac tttttagaga actaggtgaa
     9781 tttaataagg acgaaaatat agcaaaactt gaaagtgaac tattaacagc tattaataaa
     9841 cttggtatag gacctcaagg cttaggtggt actacaacag ctttgggatt aaacatagag
     9901 acttttccta cacacatagc aggtttacct gttgttgtaa atataaactg ccatgcatca
     9961 agacataaga aggtagtaat ttaggaggaa aataaaatga taaaaataac tacacctgta
    10021 aatgaaattg atatagcaaa attaaattgt ggagatacaa ttagtttgag tggtatactt
    10081 tatacagcaa gagatgctgc tcataaaaga ttgatagatt gtataaataa aggtgaagaa
    10141 ttaccatttg acgtatatgg tcaaggaata tattatgtag gacctactcc tacaaagcca
    10201 ggagacgtta taggtgctgc aggtccaacg acgagttata gaatggatga tttgactata
    10261 cctcttttag aaagaggtct tagattgatg ataggcaagg gaaaaagaag tgatgaggtt
    10321 atagaaggta tgcaaaaata cggagcagtt tatcttgctg caataggagg agctggagca
    10381 tatatttcaa attcaataaa atcatgtgaa attattgcat atgaagattt aggagctgaa
    10441 gctataagaa agattgtagt agaagacctt aagcttacag tagcaataga tagctatgga
    10501 aataatatat atgagcaagg tcgagcaatt tatgaatgta aataatgttt agaaataaat
    10561 atgaatatca aaattaaaaa tagttgtgta tattttaagg aggaaaattg atgtctaagg
    10621 attatgcaaa attggcatta gaaatgcatg aaatcaacaa agggaaagta agtgtagaaa
    10681 gcaaggtaga agttaaaact aaggatgatt tatcaacagc atatacacca ggggttgctg
    10741 aaccttgttt aaaaatacat gaaaatcaag atgatgttta tagatacact tctaaaggga
    10801 atctagtagc agtagttagt gatggaagtg ctgtacttgg attaggtaac attggtgctg
    10861 aagcatctat acctgttatg gaaggtaaag ctatattatt taaacaattt gcagatgtag
    10921 acgcttttcc aatatgttta aagactaatg atgtagatga gatagttaaa tctgtagagt
    10981 tgatggagcc tgtatttgga ggtataaatt tggaagacat atcttctcca agatgttttg
    11041 agatagaaga gagattaaaa aaatctttaa gtatacctgt ttttcatgat gaccaacatg
    11101 ggacagcaat aatagttgca gcagccataa taaactcaat aaagttaatt gaaaataaaa
    11161 aaatagaaga tttagaaata gttataaacg gagcaggagc tgctggtata gccatagcaa
    11221 agatactact taatatgaat gttaaaaata taattttatg tgatagaagt ggagcattgg
    11281 aagctagtat agaaaatcta aactatgttc aaaaagaaat gttaaaagta acaaatataa
    11341 gaaatgaaaa aggaccttta aaagatatta taaaaggaaa agatgtattt attggtgttt
    11401 caggtccagg agctgttaca aaggaaatgg ttgaaagtat gtctgagaag ccaataatat
    11461 tggcaatggc aaatcctact ccagagataa tgccagaaga agcaaaatta ggtggagcat
    11521 ttataatggg aacaggtcgt tcagacttcc ctaatcaagt caataatgta ctggcttttc
    11581 ctggaatatt tagaggtgca cttgatgtta aggctaaaga gataaatgaa gaaatgaaga
    11641 tagctgctgc atatgcaatt gctaatacaa taagtgatga agaaatatgc ccagagtata
    11701 tattaccaga tgtatttaat aagaatgtag taaaaaatgt tgctaaggca gttaaagaag
    11761 cagcgataaa aaccaaagta aatagaatat aaatttttat atgttcatat atccttatct
    11821 tatcgggtat atgggcattt ttattatagg tttatactca tatatcctta cttttatata
    11881 catatcttta tgattaaagg tgttagaaaa tgtagtaaat tttatactat cagactctat
    11941 ctttattact ttagtagata catggtggta aaggtttata gaaaaaaata taggaaggca
    12001 gatttttatt gttgcgtata tatgagaatg atataatatg aatattataa attaataaat
    12061 ttaaatttat ggaggtacaa gagatgatgg aatataaagt taatgaccaa gaacttaatg
    12121 cttcaatgtt tatcccattc gtcaacagga tatggccagg agattataat atggataaaa
    12181 cacagactgc attatcaaaa actttgaata taactgctta tgatgataaa gtgcttgtag
    12241 gatgtctgcg agttctttca gatggttatt attttggaac aattacagaa ttgcttgttc
    12301 ttccagagta tcagaaacaa ggcattggta gtaagctttt ccagcttgct aaagagaaca
    12361 ccccaactat gctatatttt ggtgcacagc caggagtaga agagttctat gaaaaaaatg
    12421 ggtgccaaag aagtctgcaa tcctacataa taaaaaagaa agatagttat taaagttcca
    12481 gctttcaata atcttaaaag tacaatattt tagtggaatc atttcatttt ttattactaa
    12541 tatgacataa acatgtcata ttagcattga tatactttta atatggttaa gagccaaaga
    12601 aaaatataag gagtgattta aaatggaaga aactaaattt tgccaatgct gtgcaatgcc
    12661 aatgggacaa actgatgaat tatatgggac aaataaagat ggaagtaaaa gtactgatta
    12721 ttgtagttat tgttttaaag atggtgagtt tacagctgat ataagtatgg aagagatgat
    12781 agaagtttgt atccctcata tgcttcaaag taataaggat atgactgaag atgaagcaag
    12841 aacaatgatg aacaatttct ttccaacatt aaaacgctgg aaataatttt atgcaaaatt
    12901 agttatatgc aaaacatgaa atatggtgtt gagaataagg ggtggtgtgc tttatagcat
    12961 accacctgtt ttgttacttg gagttcagtt attttcctaa atattttaaa tatactattt
    13021 tagataagtt ccttcttcat cttgataaat taatcctttt ttcataagca ctccaagagc
    13081 tcgtttaaaa tttttcttac ttgaattaaa taatatagaa atatctttag ggtcagattt
    13141 atcattaaat ctcatatatc catcagaacc ttctaaataa cttaaaatct tattttctaa
    13201 agtatcaagt tcatcctttc tatttcctct aggagaaaga ccaagttttc catcttcata
    13261 tatttttatg acatgtaaat tttcaagaac atcaccagtt tttagttcag taaagtactc
    13321 attatgaagt attacaccag catatttatt gtctacacat atcattgcag aattattagt
    13381 ttggaatcca tatacaactc cagttacaat atctcctaca ttgtaagtat ggtctgttaa
    13441 taagtatgaa tctatatctg tagtagcagc aattcttttg cttttgtcta aatatatata
    13501 aaataaatat ttttcgtctc taaatagtgg ataagtctta gctttaaaag gaactaatat
    13561 gtctttagga agtcccatat ctataaaagt accaatgtct gtattatcaa ctacttttaa
    13621 atgtgctaca tccccaacct tagctaaagg cggtattaaa gttgcagcag ttctgtcatc
    13681 agaatcttta aatatgaaag catttacttc atcacctatg tttatttcat tttttccgat
    13741 taatctatta tgaagaagta tatcgtcttt tgtgttatta gtttgtccat ccaaaaaata
    13801 tccaaattca gtttttcttt ttactgtaag tttattaaaa tcacctatgt ttatcaaatt
    13861 aaaatcatcc tctcatttta tatttataat ttagccataa tatacttatg tagtagtttg
    13921 tcacagtatc tatattagta ttagtgaaat ttacaataaa taatatagtt atttatgtat
    13981 ttttcaattc tctagcttct tatatagtat aaactccaat ttcttttttg tctttaggtt
    14041 ttattaaaat ttttacaaaa taagttaaaa agtattgaaa taaaatttct tttaaagtat
    14101 aattggtata taccaaaatt ggtatatacc atatcgaaaa gtggtgacta tatgaaaata
    14161 gtaaataaat ctagtaatat accattgcat actcaattaa gttcaataat aagagagatg
    14221 atagaaactg gtgagcttaa agagggcgat gcaataatgc cagaaagaga actttgcaac
    14281 atacaaaatg taagtcgtat gacagtaaat aaaaccattg ttggattagt tacagaagga
    14341 cttctttata gggtacaagg taaagggaca ttcgttgcaa agcaaaaaaa gaagtatcag
    14401 ttttcaaatg taaaaggttt tactgatgtt atgaaagaaa agggagtaaa cataaaaacg
    14461 gatatattat cctttgagat ggaactacca gatgatttag ttaaaagaaa gcttggaata
    14521 agtgataata ctacaaatat atataagatt gtcagactta gatatactga tggagagcca
    14581 tttggattag aaatagtata tctatcagaa gaaatgtgta agggtctaac aaaagggata
    14641 ttggataatt cttcattata tagggtttta aatgaaaaat atggctataa gatacaaaaa
    14701 gctgaacagg taatggagcc agtaatactt tcagatgaag aaagtaagct tcttgaaaca
    14761 gatgaaggtg ctttagctct taaacttcat agaaattcct ataatagaga aggtagccct
    14821 atagaatata ccatatcaat atttagaaca gataaatatc aatatgaaat agtattgagt
    14881 gagtaggtga ataatatgaa atgtttaatt aatggaaaga taatattaaa aaatcaaatc
    14941 ttagaaaata aagtattagt atttgatgaa aaaataattg atatagctga tagtgtacca
    15001 aaagattgtg aagttataga tgctgatgga aaatatatat ctccagggct tattgatatt
    15061 catatacatg gaaacatggg aaaagataca atggattcga ctgatgaatc tattgaaaca
    15121 atttcaaaat caattatgag acatggagta acatcttttc tgccaactac aatgactatg
    15181 gataaagaac atgtttatga tgcacttgaa gttattaaaa aagcccaaaa tagaaagctt
    15241 gaaggggcaa aggtattagg agcacattta gaaggacctt ttataaatga gaattataag
    15301 ggagcacaaa atgaaaaatt tataataaat tcaaaatacg aatttataaa agaatataaa
    15361 gatgttataa aagttataac ttatgctcca gaaaaagata ttgattttga ttttactaga
    15421 gaaataaaaa gatgtacaga tatagtttta tctattggtc atagtaatgc aaattatgac
    15481 caagctaaag aagctataaa tttaggagta actaatgtaa ctcacatgtt caatgcaatg
    15541 actggtttaa accatagaga cccaggcgtt gttggtgcag cacttacaac taatgtgtac
    15601 agtgagttaa tagcagatac aatccatata aataaagatt tatttcaatt tatactaaat
    15661 aataaaggga aagaaagact gattttaata acagattcta tagaagctgg tggtcttgaa
    15721 gatggcaatt attctcttgg aggtcagaaa gtcatagtta agggtaatga agctagactt
    15781 gaaaatggag cattagctgg aagtgtgttg tctttaaata aaatggtatt taacttctta
    15841 gataatacaa accttaaagt gaatgaagca attaatttag cttctttaaa tccagcaaca
    15901 tctttgggaa taaatgacaa aaaaggaagc ttagaaatag gtaaggatgc agatatagct
    15961 gtatttgatg agaatttaga ttgtaaaatg acactttgct tgggggaagt agtatataaa
    16021 aatatatagt atacaaaaca aaccttaaag acagtaaaaa caatataaaa aataataaat
    16081 taaacttaca ttagtaaaag tagtagttaa aatttaggag gaataagatg agagtattag
    16141 tgtgtaaaga ttatgatgga atgagtaaaa aagccgctga aatgatagca gctcaaatag
    16201 ttttaaaacc aaattcaata ctgggactag ctacaggaag tacaccagta ggtatgtata
    16261 gagatttagt aaaaaaatac aatgataata tagtagattt ttcagatgtg atgagcttta
    16321 atttagatga atattataag ttaccaatat ctaatgacca aagctatgat tattttatga
    16381 aagagaatct atttaatcat gtaaacataa agccagaaaa tactcatcta ccaaatggaa
    16441 tggctgatga tattgaaaaa gagtgtatga actatgaagc ttctatagat gcagctggtg
    16501 gtatagatgt acaagtactt ggaataggtc gtaatgctca tataggtttt aatgaacctg
    16561 atactaaatt tgcaaaaaga actcatgtag ttgaacttac agagtctaca atagaagcta
    16621 atgcaagatt ctttaaatca agagaagatg ttcctaaaaa agctgtaagt atgggaatag
    16681 gttcaatatt gaagtctaag aaaatattgc ttttagcttc aggagaagaa aaagctgatg
    16741 cagtatataa tactgtttac ggagatataa ctccagaagt tcctggttct atacttcaac
    16801 ttcataaaga cactatagtt atagtagatg aagcagcagc aagtaaactg aatccaaaag
    16861 attataaatt agtataagaa tagaatgtag tttaagtaaa aaatagctat cattgagtaa
    16921 tcaaatttat ttaatgatag ctatttttat ttaaattttt tacttctttt tatttaaatt
    16981 agttattatt gtttaaatta cttgcttatt actttttacg aaaactcgtt atcaagaagt
    17041 atccaaataa gatgttaaaa attattatgt ttagtgggat tctatcaagc ataataaaat
    17101 tacctataat tgggatatta ccaactggaa acataaagaa gaatacaggc atcagtgata
    17161 gtaaaaaaga tggaaaacaa agaagaagta atcttggaat atcaatatac caactaccat
    17221 cctttttgta ttctctaatc aataattcag ttcctagtaa taaaccaata ccaccagagg
    17281 aaattaaatt aataaaaatg tatcttttaa agtcttgcat ataaccagtg taaaattgag
    17341 tatagaaatc ataatttaat ttaacggcta aaaaatacat aagcaataga atgattaaaa
    17401 ataaaagata tctaagagct tttttcattt taaattctcc cttcagaaat actttattta
    17461 gttttatttt gttggaatct gattttatca tttagtttaa gaaatccata atattagtga
    17521 agaatgaaat aagtattata tatgaataaa tatttttaga attaaggtga a
//
LOCUS       DAKBNN010000069        17554 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000069 DAKBNN010000000
VERSION     DAKBNN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.069"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(86..775)
                     /locus_tag="OUB02_001941"
     CDS             complement(86..775)
                     /locus_tag="OUB02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCT8885699.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(768..1139)
                     /locus_tag="OUB02_001942"
     CDS             complement(768..1139)
                     /locus_tag="OUB02_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCT8885700.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(1321..2667)
                     /locus_tag="OUB02_001943"
     CDS             complement(1321..2667)
                     /locus_tag="OUB02_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCT8885701.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(2849..3442)
                     /locus_tag="OUB02_001944"
     CDS             complement(2849..3442)
                     /locus_tag="OUB02_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HCT8885702.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(3507..4976)
                     /locus_tag="OUB02_001945"
     CDS             complement(3507..4976)
                     /locus_tag="OUB02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HCT8885703.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(5087..6478)
                     /locus_tag="OUB02_001946"
     CDS             complement(5087..6478)
                     /locus_tag="OUB02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCT8885704.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(6644..6892)
                     /locus_tag="OUB02_001947"
     CDS             complement(6644..6892)
                     /locus_tag="OUB02_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885705.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            7158..9287
                     /locus_tag="OUB02_001948"
     CDS             7158..9287
                     /locus_tag="OUB02_001948"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCT8885706.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLDATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(9529..10128)
                     /locus_tag="OUB02_001949"
     CDS             complement(9529..10128)
                     /locus_tag="OUB02_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HCT8885707.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10146..13265)
                     /locus_tag="OUB02_001950"
     CDS             complement(10146..13265)
                     /locus_tag="OUB02_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCT8885708.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIHTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHLLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKGGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            13486..14310
                     /gene="pdaA"
                     /locus_tag="OUB02_001951"
     CDS             13486..14310
                     /gene="pdaA"
                     /locus_tag="OUB02_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HCT8885709.1"
                     /translation="MKKDSLKKYIMIGAFALVLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(14407..15192)
                     /locus_tag="OUB02_001952"
     CDS             complement(14407..15192)
                     /locus_tag="OUB02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8885710.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            15657..16022
                     /locus_tag="OUB02_001953"
     CDS             15657..16022
                     /locus_tag="OUB02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885711.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            16100..16654
                     /locus_tag="OUB02_001954"
     CDS             16100..16654
                     /locus_tag="OUB02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCT8885712.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            16874..17548
                     /locus_tag="OUB02_001955"
     CDS             16874..17548
                     /locus_tag="OUB02_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCT8885713.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT         5907 a         2762 c         1869 g         7016 t
ORIGIN      
        1 catttttttc gttcagtttt tattttaaat tttttttatt ttattttgtt ttaagtattc
       61 aaggtactcg tcttaatttt attttttata taaaattcat tataattgga attagtatta
      121 ctgtaattgt accagagact ccaatagaaa caccactcat agctccttct atttctccaa
      181 gttcaaaagc ctttgtagtt cctaaagcat gtgcagatgt tccaagagca attcctttag
      241 caactggatg atttatattc cccagtttga atactgtaga agcaaccata ccaccaaaaa
      301 tacctgtaaa aattatggct acaactgtta ttgattctat accatttaaa gaatttgcta
      361 aagatattcc cattggtgtt gttatagatt ttggaattag tgaatttatt atacttacat
      421 ctgaattagc tattttgcca attatcaata cagatacaaa agaaatcact ataccacaac
      481 ctattcctac aataatctca aacatatgct ttttaaataa ttcaaattgt ttataaagtg
      541 gaactgcaag tactactgtg acaggaccta aaaagaaatt tagactatca gctcctatct
      601 tgtaattctc ataagggatt ttagatatgc ttaaaaacaa tattattcct acgattgcaa
      661 ttaataaagg gttacatata ggattacttg tttttcgttg aatgtatctt cctaaattga
      721 aaaaaactat tgttactatt ataccaaata ctggtgtctg taatacatta tacatttttt
      781 tgtccttctt ccttcttttt tatcattttc tccacaataa ttccagtagt atacattact
      841 attagtgtac ttaaacatat gactaataaa agcactaata cattttccct tattaggtct
      901 aatgacttta taagtgatac acctgctgga ataaaaaata tagccatatt atcgaggaaa
      961 aatccactta cattttctat actactcaag tctaaaactt taaattgtag tagtagaaat
     1021 aataatagca taccaactat actcccagga ataagtataa ttccttgtat aaaggaactt
     1081 atatactctc ctattaccca gatagataaa attattacta attgattgaa aactttcatt
     1141 ttccacctcc aaaaattaaa gagattgctt caaatcatat atttgtagtt ttatcaacaa
     1201 gcaaatatct ttctttaaaa tagaaagtta taatttgaca caatctcttt ttaaatttat
     1261 ttttatttta atttaataaa atacatactt ggttgtttca aaaaacttaa ctatataaac
     1321 ctatttttca cttaaaattt cttttatttt tgaattgtta tacatactgt ctggaacttc
     1381 acttctcaaa ctataaaaag tctgttgtgc attttttaaa tcgccattat cataataaag
     1441 catacccaac tcgtaatatg aatcatcata atactggtca tttttagtat aagtatttat
     1501 atattttttg taatatttta tagcttcatc cttatttcct aataatttat ttgttattgc
     1561 taattgatat attgactcag aaatatattt tttagtttta ccagaagaaa ctacactttt
     1621 aaaattatct atggcttttt catattcttt gtttttcttt aaatttagag cttgataata
     1681 taaatcttcc tcatcagcta cagttatctc tctttctggt tctttatttt taacctcatt
     1741 taattcctta ttcgtttttt ctaatttttt actcgtttct cctaactttt tatcagtttc
     1801 acttaacttt tcctctttac tacttatttg gttattcaag ttttctattc tctttttgct
     1861 ttgaatcaaa tatatcccca tacctattaa aacacagcca actataatat atggcaaaat
     1921 acttttcttt ctttttactt tttttatttc ttccttttta cttgtatctt ctttttcttc
     1981 ttcatttttt tcaaatacag gattatcttt atctagctct ttaagtttgt ctaaatatcc
     2041 ttctctcttt tttacattac ctagtttatc atataacaaa gataaaatta catatggctc
     2101 tattagttca ggttcttctt caattatgtt ctctaaaata tgtattgact cataatttaa
     2161 ttcttcattt ataaatctta tagaatgatt gtatcttcct aaaaaagttt taaaatcttc
     2221 agatgaaatt agatttacat attttctaga tatatgatta ttttcataac ataaagcagt
     2281 ataaaaactc tcaaatgatt tagcaaaatc acattttaat aattttaaaa gacctctaag
     2341 atttagtata tcaatgtctt taggatttat ctccagagct ttattaacca tattaatggc
     2401 actagttata taatttttat atgctaaatc caatgcattg ttatatcttt tatatgattc
     2461 taattttaac tccttagacg ttgtatctga ctttgaattt aaagctcctt gcaagtttaa
     2521 tagagtttct tctttataat ttgtagtcac ataactctgt tttaatgctt ttaatgtttc
     2581 atcaccgtct tcttcttcta tattaaaaaa agtcaaatca cttttattat cttctatcaa
     2641 agatatatct aaaatttttc taggcatgct ttctatgtct tgttttatat ctttaactaa
     2701 aataatactc acctccactc taaaatatat atttaagata acatatttaa tatgtcaagt
     2761 caaaactata catatttttc caccattctc ataataaaaa aggtaatcct cttcttttca
     2821 taaaaaggtt acctcttttt caatactatt aacttttaat gttgaatttt tttacttttc
     2881 tatagagtgt agctatacct atatttaatg cctcagctac ttttttcata ccttctgtat
     2941 tacttccata tttctttatg gcattttcta tagcttccct ctcaaggtca tctatagtct
     3001 taacttcttg tttataaagt tcatctagtt taaaagatat taaatcagac atactcctta
     3061 caaaagcagt taactcctta tcttttgaaa gtatcttttc tttttgttct tcattaaacg
     3121 cagctatacc caagattcca agtatatgct ctcccatttt aacgggagta catatatcag
     3181 ctaactcttt acaattttct ctatttgcac atttattaca gttctcttct atttctttat
     3241 ttataacata ggatttccca tcttttaaaa caagagagta cactgacttc tctgatattg
     3301 tctctcctat tttacctctg aatattcctg tacctgctat cctagttaaa tcttttgtaa
     3361 ctaaagtaac atctacatcc aagacattag aaatattctc gcaaatactt tgaacgtaat
     3421 tcgttatatt atttaaattc aaagtaatcc ctcctattta gatttatatt ttttattact
     3481 ctacataaat cctattcttg agaactttaa gcttgcggtt gtggttgtgt ctcattaggc
     3541 ttattatcag ttggtggtgt ttctggtttt tgttgattat ttggattttg attgtttgta
     3601 ttttgattat tcttattttc gtctgtattt ggtttttcct ctggttttgg ttccatagtc
     3661 ccaactaata taataccttc cttcttagga taatatgaat tagctatttt ttcagtttta
     3721 atttttttac catttgcatc attaaatgtt ctatatgttg atacagaata tgcatttctt
     3781 ccacttttgt ctactttttc tttaccttta ggcattgtag ggtcattaac tcttttcatt
     3841 gtagtttgtg aaacgccatc taattttgtt gatattgtta tattttgttt atcttctgca
     3901 cttccatata tattgcaaac aatttgattt cctgaaacgt aattttttat gtaaactgga
     3961 tgctttagat tatttttaaa taagaaatct attccactat ctgcaacagt tgcatctctt
     4021 ccttttggaa cataacttga cggtatagta tgatttttta cattaacaat ttctaaacct
     4081 gcgtataaca cagaattgta taacgtagat gaaacttgac aaactcctcc accataatct
     4141 tcttccatct caccttccat tattacaggt gcacttttat atccattttc cttacttctt
     4201 ttccctgtat gtttattaaa tgagaaagtt tctcctggca taagtaatac attacttata
     4261 ttatctgttg ctaattttat gtttatactt ctatttattt gagaagtttt aaatgttgtt
     4321 gaatacgtac ctaaatttgt atctatatta gcaagttgct ctttttttac ttttgctgga
     4381 gtagctttta ctactaactc atttttatag ttaccttttt tagtttgatt atcaaaattc
     4441 tccatactct tagaaatatc cattttaaga ccatcagaat ctggtataac cttgactttt
     4501 tcaccactta tatcaagtgt agcatctttt acatctacat ctatttcttt agctatcttt
     4561 tccatttctg cttttaattt atttttatca taatttatca caactactaa gttgcttttt
     4621 ttgccaaaat ttgctgaaat agtttttgat agattgccaa agaaagcact ctttctattt
     4681 aaattatatg cgttttctac tgttttatcc aggtcagtag ataaatttaa atctttgctc
     4741 tttactttcc aacttttatc attgtaatta aaatcaacat tctctaattt gtatttttta
     4801 gctaattcct gttttgcttg tgatttagtt aattttccaa tatttactcc attaacatat
     4861 gtgttttttg ctattttctc ccctttaaat tgcatgcaca ttatgcctat aaaaattatt
     4921 actactaatg ctacagctcc aatagctata actagctttt tgttcacatt actcatccca
     4981 tacttctcct tacaataaaa ttatttatat atccctatta ttatattact acataatgcg
     5041 ccttaaaacc ccaaaaactt tttaattata tattaattta atattattaa tattcattta
     5101 gtccattttc tgatatggca tatatttttt cattgtaaat attaatagca taaacttctt
     5161 tgcctctaaa taagccttta tttactacag cataataata aatattttta tattttccaa
     5221 acaattcttc taaactcttt ttacttttta catatctatc tgctttctct atatctaata
     5281 tctcaatctt tttatactta ttttcttttt catcgagagt attagttaac acaaacttag
     5341 ctttatcttt tgtaatgcca aattgataac ttccatcttc taatattcta aagtatccat
     5401 caccataatc tactttgtaa tcttttttac catctttata tgaatataaa tctttagttt
     5461 ttgcacttac caaaacttca tttattaatt tatcatactc atatacttca gcataatagt
     5521 atttattatt ttttatcatt tgtggaagtg aatttataga tagatttgcc ttgtctttgc
     5581 cacttttttt agctattaca tttataatgt catcagatgt taaagtatac aatgataaat
     5641 tctttttata ttttttttct aaactctgta ccttttcatc atctgaataa tcgtctttta
     5701 aatactcaat atattgtaat gctctttcat aatctccaga agtatttgcc tcttcagctc
     5761 tacttatata gtaatcatac atttcttcaa tacattgttc tttcccattt tgagccatac
     5821 tgtaatactt ttcatcttct tttaatactt tattatactt ttcaatagct tcataatact
     5881 tatatgcttt ttggtctttt actgctgagt cataaacatc tctagactct ttgattgttt
     5941 ctatattctg tttgtataca tctaggttac tacccatacc atttaatatt gaaacagtac
     6001 ttatgtagtt aatggcaata tcataagtag tattttcttc tttatacatt tctctaactc
     6061 tatcagcttg agctaataag tcatccacat ctatacttat tttgtttaat tctttcacgg
     6121 tatttattaa tccaataaaa ctttcttgac ttatgtctcc atctaaatat ttatcaccac
     6181 tatttaatag caatttattt atttttttag aaacagactt attaaacctt tccatttttg
     6241 aatcagaaaa ctttttttct atacttgtgt agtaatcaac agctatagaa tactgtcttt
     6301 tctctataat ctttaacaac tcattagctt catttcctac actaaactcg ttactactta
     6361 agaaaaatac tattatcaaa gcaattacac ctaatgttga taatgaaatc gcaactatag
     6421 atttcctttt ttcatcaata aaatctacag ccttctcaaa taaatctcta aaattcatta
     6481 tagttccctc tccatttaat aatctttata ttttatggta aactaaattt cttattggtt
     6541 attatatcat aattcttgaa aaaaaataaa aacacaagtt aaatcgtgtt tttattttac
     6601 ctatcaatca ttattttata cataaatatt tcctttacat aaattatgat atctttcttt
     6661 ttatatccct agaatgactc ttttgttttt tattattact gacagttttc gttcttgtct
     6721 tgtgtatagc tttgctatca aatatttttc tagcattgca taaaacaatt ccaattgtta
     6781 aacaaactgt aagcacactt cccatcaaaa agtaataatc caatcctata tgtactccca
     6841 taactgaatt tattaacact aatgcaaaag tcattgcaaa acatatcatc atggagataa
     6901 ctaaaattct tagaattttt ttcaatccac tcacctcgta taatcactaa aattgacact
     6961 accccttatc cattactata ccaaattagt aaaaaaatag caattactat tgtcatttat
     7021 attaggaaaa tattaaatct ttttaataaa tatctttttt tgacataagg attacttaac
     7081 ttttaataaa atagtaacac taatatgtat taattaatag aataaaataa atgataattt
     7141 attgaggggt gatttacatg ccaaatttgc caagtttagg gtcaaaggct cctgatttta
     7201 aagccaatac aacaaatggt cctattagac tctctgacta taagggtaat tggattgttt
     7261 tattttcaca tcctggtgat tttacaccag tttgtactac agaattttta tgttttgcta
     7321 aatattatga cgaatttaaa aaaagaaata cagaactaat tggtctaagt gttgatagta
     7381 acagttcaca tttagcttgg atgtataata tttctttact tacaggtgta gaaattccat
     7441 ttcctattat agaagataga gatatgagaa ttgccaagtt atacggcatg atatcaaaac
     7501 caatgagtga tacatcaact gttcgctctg tatttattat agataataat caaattctaa
     7561 gaacgattct ttattatcca ctaactacag gaagaaatat tccagaaata cttagaatag
     7621 tagatgcact tcagactagt gatagagata atatagttac tcctgcaaac tggtttcctg
     7681 gaatgccagt gattttacct tatcctaaaa actataagga attaaaaaat agagttaaca
     7741 gttgtaataa gaaatattca tgtatggact ggtacttatg ttttgtacca gataattata
     7801 atgatgaaga agtgagcaag aaaattgata atacttgtag ctggaaaaaa gaacatacta
     7861 aaaacattga aaatgaatgt aactgtgaac atgaacatca tgactacctg aacaaagctc
     7921 ttgattgtaa acaagaacac aagactgata ttaaagatga ttgcaaccat gagaaaaaac
     7981 atactaaaaa tactaacaaa gttcacaact ccaaacaaga taagtttaaa gataagtctt
     8041 gtgatgaaat gaattttaac tatgataaag atgaatcttg cgacaaaata aattctagct
     8101 atgataaaga agatagtagt tatgaagatt tctataaaca taattataaa aactacgatt
     8161 atactagcga aaaaaatact aaaaaaatag ctatgaaaac tttaaaagat tcaaaaaaat
     8221 tagttagacc acaaataaca gacccataca atccaatagt tgaaaatgca aactgtccag
     8281 atataaatcc aattgtagca gaatatgttc ttggaaatcc aactaatgta gatgctcaac
     8341 tattagatgc agttatattt gcttttgctg agatagacca gtctggaaat ttgtttattc
     8401 cttatcctag atttttaaat caattacttg ctcttaaagg tgaaaaacct agcttaaaag
     8461 taattgtagc tattggaggt tggggagctg aaggattctc tgatgcagca ttaacaccta
     8521 catctagata taattttgca agacaggtca atcagatgat aaatgaatat gctttagatg
     8581 gaatagatat agactgggaa tatcctggaa gtagtgcatc tggaataaca tcaagacctc
     8641 aagatagaga aaactttaca ctcttactaa ctgccataag agatgttata ggagatgata
     8701 aatggcttag tgtagctgga acaggagata gaggatatat aaattcaagt gctgaaatag
     8761 ataaaatagc tcctataata gattatttta atcttatgag ttatgatttt acagcaggtg
     8821 aaacaggccc aaatggtaga aaacatcaag caaatctttt tgattcagac ttatctttgc
     8881 caggatatag tgttgatgca atggtaagaa atcttgagaa tgctggaatg ccttctgaaa
     8941 aaatccttct tggtatacca ttttatggaa gattagatgc tactataaca agaacttatg
     9001 atgagcttag aagggattat ataaataaaa atggatatga atatagattt gataatactg
     9061 ctcaagttcc atatttagtt aaggatggag attttgcaat gtcatatgat gatgctttat
     9121 caatattctt aaaaactcaa tatgttctta gaaattgtct aggtggtgta ttctcatgga
     9181 catcaactta tgaccaagca aatatactgg ctagaaccat gtctattggt ataaatgacc
     9241 ctgaagtatt aaaagaagaa cttgaaggta tttatgggca attctaatag aatgtaggaa
     9301 aattttatag ttatataaaa agcagatttt attatattaa ataagttgta tctaattaat
     9361 actattatta attgtttata aattagatac ttattttttc ataatacaat ctgctttttt
     9421 aatcaaaatt tatttaatta taagttgttt aattacaagt tgtttaatta taaagtagga
     9481 atttagatat actcttatac catgtaatta tgtataaata tttttttatt atacaccaat
     9541 agctttttta gctaatttat cagcttcctc attatactta tttccagaat gagctttaac
     9601 ctttgtaaat tttacattaa gcttattctt aatactgtca tagaaatttt tatatgctat
     9661 agttccttct ttatttgttt tccaaactcc agtacaccac ttttcaatcc cttcatagtc
     9721 aaagtataaa atcaagtttt gaacattatt gtcaatacag tactgcattg caattttaga
     9781 agcctctatc tctccagcta catttctcat acttactaaa gttttctctt ttcctttttc
     9841 actataagtt tttataactt cattattttt taaaatcaca acacccgaac catacatttt
     9901 tactgaatgt tcataacttc catctacata tgcttctata aactccttat cttcttgaaa
     9961 actcaatttt tctgacccat caatatattc tttagcttct tgaaaagtag aaaaactctt
    10021 atattcagct cctgagaatc catttacttg ttttttgcac tcatcccatg tattatatac
    10081 acctatattt ttaccttttt tcacagcata aaacttttgt tttgccaaat atatcactcc
    10141 cactcttaat ttttatcttc ttctctcata aatatatcta aatttataag atagtttcta
    10201 tcaaaatatt ttactttcat atcttttttt ataatatcaa tatggtacac ttctcctttt
    10261 acagctcttt gtcttatatt ttcatttata tcttttttta taatatcctt atcaacatca
    10321 ttgttatcca ttttagcatc aacattcaac gcttttatag tatcaaatat aacagatttt
    10381 agtattaaac ttctagattt atttaaatgt cgccttatat aagtataaaa ctcattgcta
    10441 ttaacctttg aaattttaca atcgtgtaaa tctttctcta atctctttaa aacacttata
    10501 ttagttccta catcaaaaat cacaaaacat ccatagtcat atttgcttct taaaattctc
    10561 cccatagctt gctttacctt tatggtcatt tgaggataat ttatagtata gtagtctata
    10621 ccatacttct tcattatagt aaaatacaaa gggtcttttg gatttagatt cggaagcttg
    10681 tctaaagtta cacaaaccaa accatcccct ggaatgtcta cgccttcaaa acatcctttt
    10741 gagccaagta ctacacattt tctatttaaa tcattaaggt gtctaatgcc ctttttatca
    10801 gcataaattt ctaagttaaa actatgtaag tattttttca atatttcata agttttttct
    10861 tgtctatctt tactattaaa aagagctaat gtatgacctt ctgtaatctt tgatatatta
    10921 gatataattt tactcatctc atttggaaat tcagaatttc tgtactcaca aatatcagaa
    10981 aatccaacta cagagactct atttttataa tcaaaaattg gttctataat cttttccaca
    11041 ttcttaactc tgtctattcc taatgtattt ttaaaataag acatactttc agataaactc
    11101 aaagttgctg ataaaaatat tcctttttct aattgagata aaatattttc ttcaaataaa
    11161 tctgctattt ttaatggcac tactcgaaat tcaaagtcat tataattctt atcaatttca
    11221 actatccttg cataatcatc cttttcatca tactctaaaa agatttcaaa tataatcttt
    11281 atatcctcta aatctcttgt ctttgccttt ccaaatttat atatatctgc ttctttatct
    11341 atactatcat catcaatatt cctataaatt attattatta ttgaaactaa attttttatt
    11401 attttctcac aactcaactt tatcttttca ctataggctc tataacttat ttcagtatct
    11461 ataccaccct tttttagttt tcccacaatt tcatctattt gcaaattcag ttcccatctt
    11521 aaattataat tacttacatt attatattca ttacaattac caaaatttaa tattgaatct
    11581 atctcttcaa taatgagatt tatgcttcta gcaatttttt gtttatcttc tcttccaatt
    11641 tttaatacat tataaaattt atcaaaagcc tttatttttc tcatatttct actatatttt
    11701 ttatatataa agctactatt ttgaataaac tcgtaaggat aaatttcttg taataaatac
    11761 cttaaagact ttgaatttat aatgcttgaa aagaaatcat atcctttttc agtcaaatta
    11821 tgtgcttcat ctactattat attttctaaa ggtttttcat ccttatacgg ccatttagct
    11881 aatagtaaat gatttactac tgttatatgt tcttctttta attcttctat tctgttttta
    11941 tataaacaat cttttttgca ttttttaggt ctgcacatat ttgggtcaca acttacattt
    12001 cttaagtgag tacttatttc cttgaagttg tcaaacacta aataatttat ttcttctata
    12061 tctccatact tgccaccttc aactaatctc ttcaaaaata ttaaagataa aatttctcct
    12121 ttagtagggt tttgagactc ataatcatct atatatgctt ccagtctatc tatacaaata
    12181 tagttatttt ttccctttat atatccgtaa cttacttttc catttagacc caaagaatct
    12241 aacacatttg gaatatcttt atttattaat tgtatctgca attcctttgt gtcagtagaa
    12301 attaataatc tttttttatt aattctagcc tctaatattg caggcaataa atatcctaca
    12361 cttttaccta taccagttgg tgcctctata catgcaattt tttcttcatc ttcgctattt
    12421 ctaaacaact ctcttattgt tttagtaagc tcatattgac caggtctata ttcatatgta
    12481 aatccttctt tattctccca aatagttttg tattttaata attcttcata atttttttct
    12541 tcttcattta aaatttttaa ggtttctctt tccttcttct tttcttcttt tgagtcaaat
    12601 atattatatt ctttttttat atttatatta tttgataaat cataatttgc ctcttctaaa
    12661 aatttgctcc attcccatgt tggaagatta aattttttaa gatatgaatt tattttaaaa
    12721 ctcaatggct ccaaagtagt tttttcatag ttattaaatt ttactaaaag gcaatttact
    12781 atatttatta tatcaatagc atctgataaa gctctatttt ccactttaga attacaattt
    12841 gttaaagtat tttttaagta ttctaagcta taatctttat gatatggttc taaaataact
    12901 gctaattcaa ttaaatcaat aaatttgttt tttaactttg gcatatgata ctcaaaaaac
    12961 ttttttttca gacttaaatc atgacatata atgaaactat cttctacaaa atttacaagt
    13021 ctatcttcca ccagttctat ttcattagct atttctaaat catttttatc taaattttta
    13081 catactgaga atacttctat aggtacctca aatttgtttt ttattaatga ttcataggta
    13141 tgtattttcc agtctttaac cttgacagct ccaacttcta aaatttctga atttaaacaa
    13201 tctaatccac taacttctat atctaaaaaa acaacatcat tcaatacact tattatatta
    13261 tccattttta tatcccttca agttaaacca gcctaactta tttacaatca tttctcttta
    13321 tattttatca tttatataca ctttatttaa gataattaat atcaacttta tatcttacag
    13381 taaacttctt tcatttatta tttgtatatg tgaatatttt taactataaa tattcaaact
    13441 atattataag aaatatattt taaattttaa ataagaggtg aaattgtgaa aaaagatagc
    13501 ctaaaaaaat acattatgat aggagcattt gctctagtac tttttggaat tgctagtata
    13561 aattttaaat cattagataa aacaaaaact caaatttcta gtccaacact cgacacacat
    13621 gaatatgatt ggtattttaa ccctagggag gatggtaaac agccatcacc tataaaagaa
    13681 gctgattttt ttaaaaaata cggagcttat tatgtaggaa atcccaatga aaaagttata
    13741 tatctatcat ttgatgctgg atatgaaagt ggaaacactc caaaattatt agatacgctt
    13801 aaaaaacaca atgctaaagc tcaatttttc gtagttgaaa gttatataaa aagcaatcca
    13861 gagttaataa agcgtatgga aaaagaagga catttagttt gtaatcattc aaaaagccat
    13921 ccatcaatgg caggtattac agattttgaa aaattcaaag aagaaattac aagtgtagaa
    13981 aaagcataca aagatgttac tggaaaagaa atgcctaagt attttagacc tccaatgggt
    14041 aaatttagcg aacaatcttt aaaatataca caagatttag gatataaatc aatattttgg
    14101 agttttgctt atgtagattg gtatgaaaag aaacaaccta ctcatgaatt tgccaaaaac
    14161 aaaatttatt caagaactca tcctggtgca atagttttac tgcatccaaa ttcatcaaca
    14221 aatactgaga tattagatga ggttcttaca cattgggaaa aagaaggtta taaacttaaa
    14281 acacttgatt atttaaataa taaaaaatag tatcataaat ttaaaaaaac tctatcatga
    14341 attatagtaa aaatatagtt atttataaat actttaattc tcgatagagt tttttatttt
    14401 ttatagctat ttaaaaagaa ttgtccattc atttatctct ttgtatccta tattatgata
    14461 tattttacct gctacatcat tattataaaa aagacatgga acttttcctt ccaacaataa
    14521 ttctttactc aaattgtaaa ccatataact tgcaagtcct tttcccctat atcttttatc
    14581 tgtacttaca gagaccacca ttgccaaaaa actagtttcc ataccagttg acacagtgct
    14641 aatcatagta tcatcatttc taataaaata tgctcttaca tttccctctt ttaaatgttc
    14701 tttcctttct tctatgtaat ttttacttac tttatagtct gacctattta aaagttttcc
    14761 tatttcatca atatcttttt cctgagcatt ttctatttta tactccttca tgttggaaaa
    14821 atctatttct tttatttctt ttaatacaca aaatttatta tctagttttt cataaaaatc
    14881 tttgtattta cttattagtc tatctattac acattttttt ccacttatgc tttctacatc
    14941 taaatctttt atatgattct tcatttctcc tatattaaaa ttatttttaa aactataaat
    15001 tataagtgtt gacttatttt ttaatattat agaggttata tttccatctt ctacctgaga
    15061 ccatatatct tgatattgag agctaaatcc ataattctca acgtatccaa ttatgaataa
    15121 atttagactt tcttcatgtg atacatattg ccaaaattct ttttctaaag tattatctat
    15181 ttttacaatc aagcagatat cctccttcta tgttaataat gatatacctt ataatttata
    15241 gtatcattta aaatcattat attctaccaa atattctaaa tttttaaata tctatgcaat
    15301 atgttcgtat ttatttttat attctgttac tgcttcactc caagcttctt ctactgaaat
    15361 ccccctctta tttgcaattt tttgtgctaa ttctattatt ttcatagctt ttcttgatat
    15421 attcatatat attcctcctc taaattatca taagttattt taaagttaaa tttctatagt
    15481 catgttatat atttatttac ttatatctta ctaattattt atgtactaaa tatgaactac
    15541 aatagaagaa atttagaact taataaaaca taaaatttat atacagttca tatatatttg
    15601 tcatatcaca agacaagtga tataatttat tatcatttta ctgggggtga tatgctatga
    15661 aaataagagc tgaatataca tgtcctcttg aaattgtaca tgacataatc aaaggaaaat
    15721 ggaaaactat ccttatattt gaattaagac aatctaataa aacattttct gaattgcaac
    15781 ataatattta tggaataaat caaaaaatgt tgattcaaca attaaaagag ttacgtgatt
    15841 ttgggattat tgataaaata agttctgctg gatatccatt acatgttgaa tattttctta
    15901 ctaatcgtgg taaaaaaatg ttaaaagcag tggaaataat gcaagaaata ggaatcgaat
    15961 atatgcttga acatggtcaa caagaaattc tagataataa aggaatttgc tacaataagt
    16021 aatcctatac ataccaaaaa gtaagtaatt tacatttttt acaatagtat tataataaaa
    16081 ataaatagga ggtataatca tgtctatagc tttaatattg attgatattc aaaatgatta
    16141 ttttaaaaat ggaaaatgtg aattatttca atcagaagaa actgctgaaa atgctaaaaa
    16201 aatactactg ttttttagaa aaaacaattt accagttatt catatacagc atatatcaac
    16261 agataaaaca gcttcttttt ttcttcctaa tactcatggt tcagaaataa ataaaattgt
    16321 ttttccacta gataatgaag aaattataat taaacacaca ccagatagtt tctttgaaac
    16381 agatttgcaa agtatccttg agaaaaaaaa tattactaat ctagtaatct gtggaatgat
    16441 gagccatatg tgtattgata caagtgtaag aacagcaaag aaacttcgat acgacataac
    16501 tctaataagc gatgcatgta caacgaagaa tttatcttgg aataatatag aaattaatgc
    16561 aaatactgta catcaagtat tcatggcatc actacagaac agttttgcag atgtaaaaaa
    16621 cacagataaa tttttatcta actatcacaa ataaaaacat acactatgtt aaaagagcaa
    16681 gtttgattta tttaagcttg ctctttaatc atttaaaatt agatgctttc tatcattttt
    16741 atatacataa tatacgttta tacattttat attcatatat aactattata taaattttaa
    16801 actttactat attcacataa aaaatgtagt attatagtat atataatatt atatagaagg
    16861 gggtgctaga taaatgaacc atcatatatt gattcaattt gaatatatag caagaatcat
    16921 tactgctggt ttctgtgggg ctttaattgg atatgagaga aagaatagac taaaagaagc
    16981 tggaatacgc actcatttta tagtggcatt agcttcagca cttataatca ttgtttccaa
    17041 gtatggattt aatgatatag tgggtactcc aggtattggt ctagacccag cccgcgttgc
    17101 agcacaagtt gtcagtggta taggtttttt aggtgctgga ttaatattta ttagaaatca
    17161 atcagtaagt ggtcttacta cagctgctgg tatatgggca acagccggta caggtcttgc
    17221 cattggctct ggattatact tagttggtat tgtttcaagt atcattattg ttattgtaca
    17281 gataacactt catagagata ggaaatggat gaaactccct acagcagagc aagtttctct
    17341 taaaatttct aacagcactg attccataga atatattgaa ggtaaattta aaaaatctaa
    17401 tattgaaatt ataaacatga atattaaacg ccttgatggg gattggttag aagtagttat
    17461 atatactaag ttacctaaag gatataaaaa aacaaattta ttaaaattat ttagcgataa
    17521 tcctcttata gaaactttag aaatataata taaa
//
LOCUS       DAKBNN010000070        17384 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000070 DAKBNN010000000
VERSION     DAKBNN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.070"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(21..899)
                     /locus_tag="OUB02_001956"
     CDS             complement(21..899)
                     /locus_tag="OUB02_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT8885714.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTMMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(916..1233)
                     /gene="trxA"
                     /locus_tag="OUB02_001957"
     CDS             complement(916..1233)
                     /gene="trxA"
                     /locus_tag="OUB02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCT8885715.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1383..2615)
                     /locus_tag="OUB02_001958"
     CDS             complement(1383..2615)
                     /locus_tag="OUB02_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885716.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2605..3276)
                     /locus_tag="OUB02_001959"
     CDS             complement(2605..3276)
                     /locus_tag="OUB02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885717.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3292..4128)
                     /locus_tag="OUB02_001960"
     CDS             complement(3292..4128)
                     /locus_tag="OUB02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885718.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4154..4828)
                     /locus_tag="OUB02_001961"
     CDS             complement(4154..4828)
                     /locus_tag="OUB02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCT8885719.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4842..5570)
                     /locus_tag="OUB02_001962"
     CDS             complement(4842..5570)
                     /locus_tag="OUB02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCT8885720.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKKFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5697..6386)
                     /locus_tag="OUB02_001963"
     CDS             complement(5697..6386)
                     /locus_tag="OUB02_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8885721.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6394..6957)
                     /locus_tag="OUB02_001964"
     CDS             complement(6394..6957)
                     /locus_tag="OUB02_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT8885722.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPI
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6959..7927)
                     /locus_tag="OUB02_001965"
     CDS             complement(6959..7927)
                     /locus_tag="OUB02_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCT8885723.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFISMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8028..8071)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8436..9065
                     /locus_tag="OUB02_001966"
     CDS             8436..9065
                     /locus_tag="OUB02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8885724.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9040..9444
                     /locus_tag="OUB02_001967"
     CDS             9040..9444
                     /locus_tag="OUB02_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCT8885725.1"
                     /translation="MKIRNKATNKKKKYVRISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGGADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9447..9923
                     /locus_tag="OUB02_001968"
     CDS             9447..9923
                     /locus_tag="OUB02_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HCT8885726.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10470..11135
                     /locus_tag="OUB02_001969"
     CDS             10470..11135
                     /locus_tag="OUB02_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCT8885727.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11157..12089
                     /locus_tag="OUB02_001970"
     CDS             11157..12089
                     /locus_tag="OUB02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCT8885728.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12110..12751
                     /gene="pcp"
                     /locus_tag="OUB02_001971"
     CDS             12110..12751
                     /gene="pcp"
                     /locus_tag="OUB02_001971"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCT8885729.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12871..13203)
                     /locus_tag="OUB02_001972"
     CDS             complement(12871..13203)
                     /locus_tag="OUB02_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885730.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13468..14088
                     /locus_tag="OUB02_001973"
     CDS             13468..14088
                     /locus_tag="OUB02_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8885731.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14413..15237)
                     /locus_tag="OUB02_001974"
     CDS             complement(14413..15237)
                     /locus_tag="OUB02_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HCT8885732.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYALMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15248..16651)
                     /locus_tag="OUB02_001975"
     CDS             complement(15248..16651)
                     /locus_tag="OUB02_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885733.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16655..17380)
                     /locus_tag="OUB02_001976"
     CDS             complement(16655..17380)
                     /locus_tag="OUB02_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885734.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
BASE COUNT         5987 a         2757 c         1923 g         6717 t
ORIGIN      
        1 aaagttaatc aatataaata ttaagccctc tttaatttat ctaaatagta aacagcattt
       61 aaagctgcta tttgcccttg accagctgct tttatataag aatatggttt tccaacacaa
      121 tctccagcag caaagcatcc atctatacta gtcttcatat tattgtcaac tttaatatga
      181 atgccatcaa tctctattcc aggaaccaat gcacttggag ccgtactatc ttttataacg
      241 aatactccat caacttcaat gtggttttct ttaaaagata ctttgtttac aagtttatct
      301 ccatctatct gtactggtct gtcatgtata acttcaatag aatcatctag gttatctgaa
      361 cttctcataa gattatcttt tttgtacata ggtataaaat aagtctttga agtgagttca
      421 cttaaaaaat ttgcttcttc tttagattct tcattatatc ctataactgc taccttttta
      481 tttctataaa gtggtgcatc acatgtagca cagtagccca cacctcttcc tagaaattct
      541 tcttcaccct tgattggtct agtatactct accccagttg caaggattac agtagtagct
      601 tcatacatat catttccaga catcattgta aagtattctc ccatagcata aatattattt
      661 attcttttat tttcaattga aatatccata ctatctatat gtgatttaaa cttgtctttt
      721 aattcatcac cacttatatc ataaaaacct aaataattat ctattgaagg agctttaaca
      781 agcttattac ttaaattatc attaccaaac atgataattg ttttatttct tatttttgca
      841 tttatagcag cagacaatcc agctggacca ctgccgatta tagctatatc atatctcata
      901 atactcaccc attttttata aatatgattt tacttgcatt tcaattttgt tctttggcat
      961 gaatccaatt aaagtttcta ctggttttcc atctttaaat ataatcatag ttggtactgt
     1021 agatacattg aattgttgag ctatttctaa gctctcatct atatcaactt ttacaaatct
     1081 agctttatcc ttcatctctt cacctaattc tgcaaataca ggtcccaaca tgttacaagg
     1141 tccacaccaa gttgcaaaga aatcaactac aactactccc ttactacctt caacactact
     1201 tctaaattca gatgtattta taacttttgc catttttaaa tcctcctatt aaactttatt
     1261 atccctatat aattatacta tgaaatttat aaatataaat ttacttttat aaggtctttt
     1321 tatattttat actaattagt atacccattc ttcatttcta aaaacaattt tttatatttt
     1381 tattatttta acttttttgg tattgacaat tcaaatgtag ttccaatatc tacatcagaa
     1441 gttacattta tatcaataga taagtttaaa ctaatcatgc gagctattga aagtcctatt
     1501 ccagaacctt ctaaatcttt atttcttaca ttttcacttc taaaaaatct atcaaatata
     1561 agaggtatct cttctttttt tatgcctata ccagtatcac ttataagtaa agttacctca
     1621 tcctctatat cttctttcaa ttctaatgct atctcatcac ccaattttgt atacttaaag
     1681 gcattatcaa tgaaaattaa tataagttgt cttaatttat ttttatctgt tattataact
     1741 ttattcttca gtttagaatt aaacacgaat cttttttcct gaaactcagc aatgtcaata
     1801 tagtcttctg atatttcttc caataacttt tctaaatcta tttcttctaa attaacttta
     1861 acaatagagt cctctttagt aagagacaat aaatcagtta tcattttttt aagtcttcta
     1921 gtttccttca ttgcagtagc tattgtttca acttcatcat ttactgtact ttctggggat
     1981 tttaacatac tttctaattt tgatgaaact attgttattg gtgttcttaa ttcatgagaa
     2041 gcatcttgta taaacttagc ttgatttttc catgctgttt ctattggaat taaagctttt
     2101 ctagtgagat atacagccac aaagtaagta attattacag aaattaatat tccaattata
     2161 agaacagatg taagctgtct taaagaattc atctcagaat ctatatttct aattatctgt
     2221 atttggtatc tacctacatc tacatttaat tctctaaaag tatatccatt ttctgtgtat
     2281 ttaaaaaacg aatttttttt atctagtctt ctatctggaa gtaactcatc aaaatattca
     2341 ttttgagtat agtaacttat ccttccatct tcatatacat acaccatatc tttaggatct
     2401 ttcagtctta ttgggttcaa aaatgaagtt cttttaaatt gagagactat atattcaagt
     2461 tcatcattta tctcactatc aattccacta tatgtaagtc ccctaaaata tgagtatata
     2521 aatatagaaa agattattaa aaaactacct actacaataa tatacatttt tattaaatga
     2581 tttttagtac tggtaaatac attcttattt atcatcgaat atatatccta ccttacgttt
     2641 agttttaata tatttatcat atccatattt acttagtttt tttcttagat tacttacata
     2701 tacttccact atttcaatag tcgcatctga atcaattccc catattctat cataaatctg
     2761 ctcttttaat aaaattgaac ctttatttaa tacaaaatac tcaagcaagt taaactgttt
     2821 gttttgaagt tttatttctt ctttttcaat atacactctt ttttctagtg tatctaaata
     2881 taaagattta aattctaatc cgtttctttc ttttattttt ccattagtcc ttaatatagc
     2941 gtatacccta gctactaact cttccatata aaaaggtttt gtcaaataat cattagctcc
     3001 tatagtaaat gcttctactt tgtcatctaa tgtttctttt gctgtcaata taagtacagg
     3061 tgtatctata ttattaactc tcattttctt taggatatca aatccattta tattaggaag
     3121 cattaagtct aaaatgacta aatcatatat attttgattt actaagtata aagcttcttc
     3181 tccatcttca cacttttcag tttcaaaatg cttgctaagt tcttctacta cactttctaa
     3241 aagtatctta ttatcttcaa ctattaaaac cttcataagt atcccctacc tttatttgtc
     3301 tatcttaact ttatgagatt ttaagtcaat atccacattg gctttttctt ttatatcatc
     3361 atacgtatat cccactattt caacactttt tgcctttgtt gaacacaata tatctatatc
     3421 tgtctcttct gaaggcttta aaatcttata tgatggcaca cgatttactg ttaaatattc
     3481 tccattttca ttaacttctg ctgtcttaag aataatattt cctaaatctt ttgatgatga
     3541 attttttact tttacttgat acgtaattcc ctcatttgat tcatttactt ttttaagttc
     3601 tgatgtagat agcacctcat actgactact attttctagc ttaatattat tatttcttat
     3661 attaatagta ttttctttta aattaacata cacaatctta ccctctgcct catattcata
     3721 tgcataaact tctacaccct ttacaaaagt tttatgccca cattctatgt aagcaacttt
     3781 cccaggcatt aaagtcatgt ccaacaaagc cttactatcc gaagatataa tttttttatt
     3841 ctcatcatat tcattatata tcaattcaac attggatata ttaaatgttg aattattttc
     3901 tacctttgtt gatgtatcta ctttatcacc ttcatctata tttataattt ctgattttcc
     3961 tatattcaat acttttgcat ttccctcatc tatttttatc tcatttttat cttctgtttg
     4021 aatatcaact gaatcaggct gttcatcttt ttcacatgct gtcaacataa aaataaatat
     4081 tgttaatata atcattagag aaattgattt ttttactatt ttattcatat taagcacctc
     4141 atatagtagt attttatagt agatttttat actttttcat aaattctttc aatgcctgtg
     4201 taagtatttg atgtttttca aaattcgaat tactacataa tctttcaaaa tcattccata
     4261 tttctttatc tatacgtatt cttgttgttt ttaattcatc tatgtgttca tcatctatat
     4321 gtacatcttt tttattagat acttttttag gctttatatt gtaatctttt acttctaaat
     4381 accaatcata tacttcacaa agcttatcta aatcttcttg agttatattt atttttctat
     4441 ctttcttagg cttaatattt tgttttggct ttgcattttt taagcctgta tcattaaact
     4501 caatttgttc aacactttta cttaaagcct tttcattaga aatctttttt ttggtgctta
     4561 ctttttttct attacttata gtattctttt tacttttttc atactcctta gctttctctt
     4621 cttccaagtt tctattatcc aaacttttat tataatttaa ttcgtctttt aatatgtatt
     4681 tacctttttc tgacttatat ccttttttat ttaaaaaagt tcttaaagta ctttgactaa
     4741 tctttagatt ttgagctatc tctgcaaatt taaatccttt gctttgaagt tctaaaaatt
     4801 tgtcaattct gtcttcattg ttattcatta atattcctcc gtcaacatct tcttgtcttt
     4861 cttttcatac ttcttattat attcttcttt tctaaatcta ctctatttga ttcaattatt
     4921 ttttgtagca ttttattata agtgaaatca tctaaattat tattttttat atagagaaaa
     4981 gtttcctctt cacatttaat aaaacaaatt gatagcaacc atgccatacc cattttaaca
     5041 taatattctt cactttgtat attattacag tattcaaata tttctttaat atgtttatca
     5101 tcaacataat atattaaaaa cataactaat gcaaatctaa tttcccatgg attctttgaa
     5161 taaacatatt ttttaagtat atcatacata ttttctttat attttttcat agactttaaa
     5221 tcagaacaaa atccatcgca aactgaccaa ttatcgattt taggtataaa tctttttaca
     5281 tagtataaag tttcttcaaa attactattt atattgccaa ttaccatacc ttgtagccta
     5341 acttcttcat agtatgtatc ctctgctact tctaaaaatt tcttccaatt tccttggctt
     5401 atttcttttg ataactttct taaattagga actctaatac ctattatatt ttctgttcca
     5461 ggtataagct ttttattaaa atctctatat tttatatctt gtatagacat catatattct
     5521 aaaagttcat tataatccaa tttattccat tttcccctat caaacatcat ttaattgctc
     5581 cttaattcct tttttcttat tgtaacataa aactctacaa aaaaagcttc taactattag
     5641 aagctttttc aaaaacataa gaaacttttc aattttaaat ttaaactaaa ttaatgctaa
     5701 tctcctacac caaagtctgt attattgtat aaaacttcta tatttggatt ttcatccaag
     5761 aatttatact tactaacaaa ccatttcact aactcttcat ctctttttat actagaagaa
     5821 ttatttataa aaacattcac tgtcgctttt tcaaaagtat ttaggataat ctctatatct
     5881 ttatctatca tctcttttgt ttgtcctttt attccaacca taatacaagg tgaatcaaaa
     5941 tattctttta catcttgtgg agtcttaaaa tttgcatttt tatttaaaat attatttcta
     6001 aaatcattgt caaaggtttc aactccaatt ttaaatacca caggaatttc aaaatactct
     6061 ctcatttcct taagcctatt tttgtaagac caatgacttt ctaaaaataa tttttttata
     6121 tttttctctt ttataataca ctttattttt tctaaagtat ccttaggtaa ttcaaaacag
     6181 cttccagaat ttatgacttc aagaactcca tatttcccag ttacgttttt taaaaccttt
     6241 agatttaatt tattcatctc ttcttctaaa ttagaattat catctatata atcacaaaaa
     6301 gaacactttc cccatataca aggaaatcct ttaagcagaa caatttctct ttgattttta
     6361 tttgttatct cactatatct atccattttc tatctatttt cctccagtca atgatacctt
     6421 taaagttttt ggaactgcat ttaatcctaa attcactaaa acaactgcta acagcttatc
     6481 tccataatca gtaaatactt gggttacaaa cacactaaaa acatctccca gtcctaacac
     6541 tttcaagatt tgcactatat atgatgaacc agaagaggtc atacctccaa ataaaaatgc
     6601 tgaaattatt gaacttatta tagatgttgg aatggcaaat aaaagtactc ctattggagt
     6661 ttttaaacct cttaaaaaat tctttttaaa tgctattcca gcaagtaatc cggttgatat
     6721 ttgaactggt gcaaaataga gagaatacac atcaaacgta attccactta ttaaacttcc
     6781 aaacacacct gtaacaacag cgtatttagg tcctaaaagt gaagctatca ttatagttcc
     6841 tattgaatct aagtatacag gtaatcttaa tcccatagct ataaaagcgc ctattatatt
     6901 tatagaaata ccaaaagcta taagtgtaat agtttttaca catatttttc ttttcacact
     6961 atatcactcc ttccacagag tctataatgt cttcataacc tttaaaaagc ctctttaaaa
     7021 acatgtacat aaatttcttc tcatcaactt catttagaac aattgcatta ggattttttt
     7081 tatagaaatt gaaagaatcc actatagact gacccatagc tattccatct tctacaactt
     7141 ctacatatga ttcaaatcct ttacatatac ttctatctat aaagtatgct actgctagag
     7201 ggtcatttat cacacagcca attattccct cctgttccca atggaaatca atataaaatc
     7261 tagttatttc agttatatac tttgccatct tcttatcaag tctatttata aactcaataa
     7321 tattaggagt aagtacaatt tttctagtta catctaaacc taccatgtgg atttttttag
     7381 ataaattctt gtaaacataa tctgctccat gtgggtctac ccaataatta aactctgcta
     7441 ctggagagca atttccatga atcctaaatg ctcctcccat agatataaac tcatcgagat
     7501 tttcaaatgc tttcttatct ttaataagtg ctttagctat gtttgtaagt ggtgcaagtg
     7561 ctattattga tactttttca tgattatgta aagtatttat tataaaatcc actgctccat
     7621 tttttgcttt agctcccaca accttttgat aaaagttttc tccaatacca tcttctccat
     7681 gtgtatcttg agctgttaca agttttcttt ttagtggtgc ttcttctcct atatatactg
     7741 gaatatttag tgaagaacac atttgaagtg tttttagtgc attttctgca cctatatttg
     7801 ctggaacatt tccacaacat gtggtaattc caatcacttc tagctctggt gagtttaaag
     7861 ccagaagaat tgccaaagaa tcatcaattc ctgggtcaca atcaattatt acttttctct
     7921 tttccatttt ttatcctcct tgatttgtgg tcgaggagaa ctacacatct tttttccact
     7981 ttcaaaaagg tgtgtctaag ggtattaatt atatactgcc actatataat tccttagttt
     8041 tttatacttg gagttctcga acaagtacag atgtaaaatc atatttttag tctacttact
     8101 atagactctt aaaaactata gaccgttaaa aaatttttaa acatctgatt taatagctat
     8161 aaaataaatt ttatagcata aaagaattta ttatatttta gtgtatctta ttaaagttta
     8221 tttagaatta tgaaccaact ttaataagat gtggcgcatt taaaccgatt attattatac
     8281 aataatatct taaatattac aattaataac attaatttat tatccataaa aatatattaa
     8341 cttatattat ttaataattt attgacagta ttaaaattaa attatacaat aacagtgtaa
     8401 attaacttaa caccgttaag caaagaggta attatatgag tatcaaagaa agacgtaaaa
     8461 atgaaaaaga agaaatgaag aaaaagatta tggatgctag catcgaaatt ataaaccaac
     8521 atggatatga aaatttatca ataagaaaaa ttgctacaaa aatagaatat tctccaacta
     8581 caatttatct ttactacaaa gacaaagcag aaattatcag cgatatgacc aataaacttt
     8641 ataacacagt agaaagtaat gccattgaca ttatgaataa ttgctcatca ctaccaatag
     8701 ataaacaaat catagaaatt atgactagct ttataaaaac tctttctagt gaaccagaga
     8761 tggcaaaatc tatcatgcat agtagaatga atattatatt tgcatctgaa aatacaaaca
     8821 atacgcctag caataatggt ataaaaatgc ttgataaatt cctttccatt ggaattgaac
     8881 aaggaatatt caaaaaaaat atagataaaa gctcatggat gcttataagt gcattgttag
     8941 gattcgtttt atgtgttgtt gaaaataaac tatattcttt acataatttt tctcaactta
     9001 tagaaaattt cttagatatt ttagtgggag gactttacaa tgaaaattcg caacaaggct
     9061 actaataaaa agaaaaaata tgtaagaata tctattgctc tattctttgt atttatatgc
     9121 attattattg taattgtaag caacttaaag ccggaaaact tggtagtaaa agatatagat
     9181 attaataatg ttaaaaatgg catttatact ggaggtgctg ataataactt ggtaaaagct
     9241 actgtgtctg ttgaagttaa taatggtaaa atccaaaata ttaatatctt aaaacacgac
     9301 catcttttag gtaaacctgc tgaaaaaatt acaacaagta ttattaaaca acagtcttta
     9361 gatgtggatg ctataacttc tgcaacttat agcagtaata ctatacgaaa agctgtagaa
     9421 aatgctttaa gaaagggaga ataattatga atactattat tatatattca agtaaatatg
     9481 gttgtacaaa agactgtgca aatatactaa aaaataaact atcagataat gtcactattg
     9541 ttgatattaa taataataat aataaaattg agttatcaaa atttgataaa attattattg
     9601 gaagttctat atatgttggt tcagtttcaa aaaaaataca agtgctatgt aatgataatg
     9661 tagagttatt aaacaaaaaa caagttggca ttttcctttg ctgtggcttt tctgaacaag
     9721 cagataaata tttaaaatca aattttccat catccttatt agaaagtgca aatgctattg
     9781 gaatttttgg tagtgaagct cgattagaaa aaatgaaatt tttagataaa cttattatga
     9841 aggctgtttc aaaaggtaat tatgacagtt ttagaatatc acaggataat atagataatt
     9901 ttttaataaa tcttaatagc taaaataaaa ctaattatct ctatgataaa ttcttttatt
     9961 tataagtaat attgctcaaa atagtcttta aactttatca aaattttatt tttcttagtg
    10021 aaatctaatg aaactagcac ttaatatatg ctttattaat cataacaaaa agagagtgtc
    10081 taatgaacta aaaaattcat tagacactct ctttttgtat gaaatcaaat atatttttat
    10141 catttcaaaa gtgaaaaata cacttgtctc tatgtttggc acattctctt tttataatat
    10201 aatatctaat ttaaaaccta tttcaaatat tgacatatac ttaaactata gacttacaaa
    10261 atcaattcat attagcttaa aatataatct ttgttattca aattcctata cacaaccatt
    10321 atttactttg atacttaaaa acatcttccc tattttttat taaattttat aataatagtc
    10381 ctattaacgc aaatactata aaaagagttt aactttatcc attaaataaa tttaaattaa
    10441 aacatataga aatggaagga gaacaattta tgataaaact aatcggtata ttgatagttg
    10501 taattggatt tatacttaaa attgacacac tatttactgt attattagca ggagtggcaa
    10561 caggaattgt tgcaggatta gattttaatc aaatttttac aattttagga gattcttttg
    10621 tctcaaacag aggtgtatca ttattcatat taacattgcc agtaatcggt gtattagaaa
    10681 gatatggttt aaaacaaaga gctgtttctt taatagaaaa attaaaaaga cttacaacag
    10741 gaaaagtact tactatatac atgatagcta gacaaatagc tggtgctcta tcaataagaa
    10801 tgagtggaca tcctcaattt gtaagaccac ttgttaatcc tatggcacaa gctgctggtt
    10861 taagtaatag tgatgaacta aaagaatcgg atgaagaagc cataaaggca ttatctgctg
    10921 catcagaaaa ctatggaaat ttttatggtc aaaacttatt tgcaggtagt agtggagtat
    10981 tacttatagc atctactctg actcaatttg gatacaatgt aactggactt aatattgtaa
    11041 aggctagtac tattatggca gtaatagcac ttgtagtggc tacaatccag tttaccttat
    11101 atgataaaaa attaaacaaa agtcataaaa aatagtttta tttaggagga ataaaaatgc
    11161 aaaattttat aaacatatca ttagaaatct tttacgcact aatgggattt ttaatgattg
    11221 ttatagcata taagtctttt actaccataa ataacaataa gaaatatggt acatcattat
    11281 tttggatact tatatcattg ccattcatat ttggtagatt gattccagcc aatataatag
    11341 gaataatatt aatattatct agtttattaa cactttctaa acaagttgta tttgcaaagt
    11401 atgaagaacc agatgaaaat tttggaaaag aacaagcaga caaacttaaa aataaaatat
    11461 ttataccatc attaatatta gcatttgctg cagtagtagt agctatgagc ctttctaatt
    11521 ttgataattc ttctcaattt gcaataggtg tgggttcaat aattgcatta atatcagccc
    11581 ttataataac taaggcaaaa cctgctacat cagttcaaga tggctcaaga ttacttcaac
    11641 aaatgggacc tgctagtatg ttaccacaac ttttagttgc attgggagct ttatttactc
    11701 aagccggagt cggtgaagtt atttcaacca tgatatctgg tgtagtccct gctgatagca
    11761 gattatttgg tgttatagct tatgtattag gtatggttat atttaccatg ataatgggaa
    11821 atgcatttgc cgcattttca gtcataacgg caggaattgg tattcctttt gtattgtctc
    11881 aaggtggaaa tcctgctata attggagcat tagcattaac agcaggatat tgtggaactc
    11941 tacttactcc aatggctgct aattttaata tagttccagc agccttgcta gaatgtaaaa
    12001 atgattatat tgttataaaa tatcaagctc cagttgcttt ggtattaatt atagcccaca
    12061 tattagtaat gtacttttta ggattttaat ataattagga ggtaaaataa tgaaaatttt
    12121 gttaacagga tttgacccat ttggcggaga accaataaac ccagctcaag aagcagttga
    12181 aagggtcaat aataatataa acggtgcaga aatcataaag ataactatac caacagttat
    12241 gacaaaatct gttgaagcta tagataaagc tattcaagag cacaatccag atatagtaat
    12301 atcagttgga caagctggag gtagatttga cattacacct gagagagttg ctataaatat
    12361 agatgatttt agaataaaag ataatgaggg taaccaggtt atagatacta ttataaaaga
    12421 agatggtgaa cctgcatatt tttcaaaatt gcctgtaaaa gctatggtaa aacatatgaa
    12481 tgaaaataaa attccagcta gtgtttcaaa cactgcagga acatttgttt gcaatcatgt
    12541 gatgtatgga attctttata tgatagacaa gaaatatcct aatataaggg gtggatttat
    12601 acacatccca tatacaactt cacaagtaat agataaaaaa aatacaccat tcatgtcttt
    12661 agaagaaata gtaaaaggct tagaattagc tatagaagct tgtattatat ataaagaaga
    12721 tgtaaaagaa attggtggtg aaatttcata ataaattttt tagtattttt ataaattaaa
    12781 aatactaact attaatttaa atatagactg tgagtagatt attcaaacaa ttttactcac
    12841 agtcttttta tggtatctac agatacaaaa ttaaggtcta ttcctctctc cccactcaca
    12901 cattgcatct aaaattggaa tcagagattt tcccctttct gatagactat attctacctt
    12961 aggaggtatt tgaggatatt cagtacgaat aatcaagtta tctgcttcta attcttttaa
    13021 tgataaactc aaagttttat aggagatagt atttatataa cgttttaatg cattatatct
    13081 tacaacttca aattctacaa gacagtaaag aattatcatc ttatatttac cactaatcag
    13141 tgaaagtgta taactaaatc ctgtttcact taaatctgta tttgatatac agtctatact
    13201 catatattac actctccttt tggttagtat tgaacaaaaa tgtgcgtact tgtttttaga
    13261 ataatttata ataaaattat agtgtaagaa taattgaaag tcaattttat aataggaggt
    13321 gtatttacta aaaaaaagtt attttaaata ggattctaag attagacatt tctatataaa
    13381 ttgataatat tcatgaaaat cctattttat aaaaaataat atctcttaga aaatctatta
    13441 aataaaattc attggaggaa atttataatg aaagttttat tgataaacgg tagcccaaac
    13501 caatatggat gtacttatac agcactaaat gagattacta aagtattgag taaacacgat
    13561 attaaaacag aaatactgta tttaggtaaa gaaacaatcc caggatgtat tagttgcgca
    13621 tcctgttttg aaactggaaa atgcattcga aatgataaag ttaatgaatt gattgaagat
    13681 ctggataata ttgatggtat tattattggt tctcctgttt acttttctag tgcaactggt
    13741 caattaactt cttttcttga ccgtctattt ttcatagctg gaagtcgcat ggctacaaaa
    13801 ttaggtgctt cagttgtgtc atgtagacgt ggtggtgcaa gtgcaacatt tgaccaacta
    13861 aataaatatt tttcaatcag caatatgcct attgtatcat cacagtattg gaaccaggta
    13921 catggcttta ctccagagga tgttatgaag gatgaagaag gattacagac tatgcgaact
    13981 ctaggagaaa acatggcatg gcttcttaag tgtatatcag caggtaaaaa agctggaatt
    14041 aaagaaccac aatatgaaga aacaattatg actaatttta taaaataaat aaaaaatgta
    14101 ctaatattaa ttaaaacaaa acactaattt ataaataaaa taagcataaa tgtaggataa
    14161 aaagcactaa aacagaggta ttgaatttta tatcaaacct ctgttttatt attacattaa
    14221 taaccaaacc atgtataaca tcatatattt ctaaataaat tataatatta tattctaatg
    14281 ttattgaaat tacaataact gaatttctgc tcataaattt aaatattcta caaatctcat
    14341 ctaaataaca attttaatag acctaaaaca atcctcatat tgttcatttt gtattaaaaa
    14401 tatcacttac aatcaaaaac catttattaa gtatattcct aagatataca atatatatac
    14461 atggattgga tagtataaat aaaataaata cttcatactc ttcccttttt ctccattgta
    14521 aagtaacatt aaaggtagag aagcaatcat catccattgg taattttgta aaatagaatt
    14581 ttttaaacta taatctccat ttgacattgt tgtaaatata aatatacaca ttaaagcata
    14641 acaaatactc aaactgaact ttttccctct acacaaataa aaaataatcc caagtataat
    14701 ccaaattggt ccaccttcta ctaaaaatgg tactggtacc aaaatcataa ataaagcaat
    14761 aactctgtta gtgactttaa aaagtaaagc tactgtaaat attcctaaaa ttataggaat
    14821 gattatcatc aataatccta taataaaata tttaatttct ttctcttttc taaatttctt
    14881 tatgaactct attgcttgta aataaatcgc tattaaaaat aatgttgaaa aaatgttatt
    14941 tataattata gctccttctg gatgtgaaaa tacattatta attatttgat ttcctatagc
    15001 cataataaca gaccaaatat acaatctact tatatatttt tttctgttac tagtgtgata
    15061 aaatccttca acagccataa atataaacag tggtgctgat attctgccta cccaatgaaa
    15121 ccaaattggt atttgcattg tttgtggcat aaatgctgct atatggtcaa atgtcataaa
    15181 tattaatgct aaaatcttaa tagtaaaccc acttatacct tttttaaaaa aatccatatc
    15241 atatctccta attttgtttt atctctataa taatttttgt tcctattcca atttcactac
    15301 ttacataaat tttaccttta tgaatctcta ttatttcttt agaaatagcc ataccaagac
    15361 ctgaaccttt atgcatctca cctgtattag tacctctata atatttttta aaaatatgtt
    15421 tcaaatcact tttgcttatt ccaattccat tatcttttat tataatttct atattatctt
    15481 tttttacaat ttctacactt atcaaagttc cttcgctatt atgaactatt gaattaaata
    15541 taagattggt tatagccctt ctgaataaga ttgaatctac atatacttca attaaatctt
    15601 cattactttc aaattcaata ttcctatttt tatatctatt atcatttaaa atatcaatta
    15661 ttatatctct gactaaactg actaaattta tttttttctt gtccagaact atagtattat
    15721 tttttaatcg agtagataaa tttaaatcat caactaaatc ttttatatac ttagacttat
    15781 tatatataat ttctgtatac tcttgtattt catcttcttc aaactcataa tctttgtcat
    15841 taattatctc tgcataccct tgtatagatg ctagaggtgt ctttatatca tgtgttatat
    15901 ttgccagcca ttcctctctc aactcttcat tttcttttct ctcaacttca tttactctta
    15961 aagtatcagc taacatattt atatttttaa aaacttcttc atatatacct ctgtccttaa
    16021 gatataaact ataatttcca ttagccatag tctcaatact ccatagtacg cttgaaattg
    16081 gttttgtaag ttttctacta aataaatatc ctacgcctaa agctaccacg ctatcaatta
    16141 ctagtgttat aatgataact ttatttaaag tttttttaat gttattttga gaataggtta
    16201 aaattattct tgatatatct ctactaggta tacctactat tatgtttaga tgttttttgt
    16261 ttaaatattt ttctagtaca aaattaacat actttgtttc tatatacttg taattattca
    16321 ccatttgaaa gggtgtatat ttttttggag tatcctttgg cacatttact ccataaactt
    16381 cctctcctaa atcatttaaa acctgtatcc aggaatttga tttttctaat ttttcagctc
    16441 caatcttaga tagtttaaat tctccatctt tttcatataa atctttttca aaagaacgtg
    16501 caaattcttc aggattattt ccagaatcta caactttaaa aaagctatta gttgaaatca
    16561 catacaatat agatatgata tttataatta ctactgatat agctacaaac acaattgtaa
    16621 atataaaatt tctagtaatc ttccacttca tattctaatc ttcctttact gctaatttat
    16681 atccaagacc ttttattgtc agaatatatt caggttttga aggattatcc tctaattttt
    16741 ccctaatttt tcttatatga accattatag tattgtcaaa tccaacataa tcttctcccc
    16801 acactttatc ccatagtttt tctttactta taatttgatt ttgattatgt gcaaataaag
    16861 ataggatttt tagttccttt gcagacagtc ctatagattt tccatctttt ataagttctg
    16921 ccctactttc atttaatata tatgggccaa atgatataat tttttcttca ttattattta
    16981 gatttttact ttcgttttga gcatcactaa aataacttaa tcttctcaaa tgcgctttaa
    17041 ctctaaaagc cacttcctta gggctaaaag gttttgtaac ataatcatct cctccaatcg
    17101 ctagtccaag taacttatct aattcttcgg ttttagctga taaaaatatt attggagaat
    17161 tggaggtttt tcttatttct ttacaaatat cataaccttc gccatctggt aacataatat
    17221 ccaatacaat caaatcagga ttaatacttt taaataattc taatccttct tttttagttt
    17281 ttgcagtata tatattatta aggccttctt tttttaggac agtttctaaa agcttcaata
    17341 tttctacttc atcatctatt attaaaacac gattttccat aaac
//
LOCUS       DAKBNN010000071        17318 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000071 DAKBNN010000000
VERSION     DAKBNN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.071"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(207..1226)
                     /locus_tag="OUB02_001977"
     CDS             complement(207..1226)
                     /locus_tag="OUB02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT8885735.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1402..1941)
                     /locus_tag="OUB02_001978"
     CDS             complement(1402..1941)
                     /locus_tag="OUB02_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HCT8885736.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(2042..2722)
                     /locus_tag="OUB02_001979"
     CDS             complement(2042..2722)
                     /locus_tag="OUB02_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCT8885737.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2751..4040)
                     /locus_tag="OUB02_001980"
     CDS             complement(2751..4040)
                     /locus_tag="OUB02_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCT8885738.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMIAEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(4488..6089)
                     /locus_tag="OUB02_001981"
     CDS             complement(4488..6089)
                     /locus_tag="OUB02_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCT8885739.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDDILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(6200..6877)
                     /locus_tag="OUB02_001982"
     CDS             complement(6200..6877)
                     /locus_tag="OUB02_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885740.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(6890..7585)
                     /locus_tag="OUB02_001983"
     CDS             complement(6890..7585)
                     /locus_tag="OUB02_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HCT8885741.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(7637..8836)
                     /locus_tag="OUB02_001984"
     CDS             complement(7637..8836)
                     /locus_tag="OUB02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HCT8885742.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVEGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(9097..11142)
                     /locus_tag="OUB02_001985"
     CDS             complement(9097..11142)
                     /locus_tag="OUB02_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885743.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQELEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(11552..12362)
                     /locus_tag="OUB02_001986"
                     /pseudo
     CDS             complement(11552..12362)
                     /locus_tag="OUB02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(12446..12979)
                     /locus_tag="OUB02_001987"
     CDS             complement(12446..12979)
                     /locus_tag="OUB02_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCT8885744.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(13129..14478)
                     /locus_tag="OUB02_001988"
     CDS             complement(13129..14478)
                     /locus_tag="OUB02_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HCT8885745.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGADLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(14490..15872)
                     /locus_tag="OUB02_001989"
     CDS             complement(14490..15872)
                     /locus_tag="OUB02_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HCT8885746.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFVVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16116..>17318
                     /locus_tag="OUB02_001990"
     CDS             16116..>17318
                     /locus_tag="OUB02_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCT8885747.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDR"
BASE COUNT         5702 a         3116 c         1775 g         6725 t
ORIGIN      
        1 gtaataaaat cgtcaaaact ttttttgata ctgttacaaa attgtaaaaa aaaagaaaaa
       61 aacgccaaaa ataaatttct ttttagcact ttttcatact tataatttga cattttaaca
      121 cttggttaag tatacgcctt accttaactt aaaattaaat ttatttaaga atatatatta
      181 taaattctta tattttctaa tataaactat atactatcag ctggataaac aatactactt
      241 tgtcttctta tctcatccat aatttccatt actattattg aattttcatg agaatttata
      301 tttgattcta ttttattgtt atttataaga cttataaact ctacaagttc ataatacatt
      361 ccttctattt ctctaggctt ttcctctgct ttttcttttg gaatagatat atcttcttct
      421 cttccatctc tgtatttaat aatagctttt tcaaatagat taagcttttc aattattata
      481 cttccctctt ctccttgtat ttcagatggt atataagaat cagctacttt agaatactga
      541 attatcgcat ccatatcatc atattgaaat acagctgaac cttctccatc cactccagtt
      601 tgaagtatat gagaaactgc ctttacagtt tttggagcac caaataaatt caccatagga
      661 tgtatacaat acactcctat atccattaaa gctccatttg acaactcttt tttaaaggca
      721 ttttctataa ttccattttt atatttatca tatcttgaag aatactgaca atagcttcca
      781 aaaaatcttc ttattttccc tattttatat aagttttctt taactgcttt aaagtttgga
      841 acacatgtaa ttctcatagc ttccataagc aatacattat tatctttggc tgtttttatc
      901 attgatttga cttcttttaa attagatgct aacgactttt cacaaagcac atgttttttg
      961 tgttttaaac aagtaatggt ttgctttgaa tgtaatgcat ttggagaagc tatgtataca
     1021 gcatctatat ccttcgattt agccatttgt tctaaatcat caaaaaaaat acttgctcca
     1081 tatagagttc caaattctct agctttttct aaatttctag aatatacagc aaccaattca
     1141 aagtctttaa ccctagatgc agctcttaga aatatatttg ctatattgct tgtcccaaca
     1201 ataccaaatc ttattttacc catcataaat cactccccat tttctacact ttattttaat
     1261 tttaataaaa aactttacaa cttatagata tattataaaa taattttatc aaataaaaaa
     1321 gaaagctaat tttaaatgat ttcaccattt taacatagct ttcttttaac tacaatctaa
     1381 attcaactta tatttttaaa gttatctttc tactatatct ttatttcccc tcaaaaattt
     1441 tatagtaaga atttcatcca caaacaatgt tactaaaagt atagttacaa caattggcca
     1501 ataaggtatt ataagactat ataaactatc aactatatac acttgaaaat atctaactcc
     1561 aataaatgtc aatatagtcc aatatacaga aaactttaga catactattc ctctactatt
     1621 gtacttataa tctgaataat cccaatagac cttattaaaa tatactttca taagtattcc
     1681 tgtaatatat tctactactg tcggcactac tagacataat aaaagcaatg caaatatatt
     1741 attggggtca ataaatcttg agacctctat tataatcgac attgcaattg catacattgg
     1801 tttaaatgga ccatttaaaa atccatcttc ttgaaaatgc ccttgtttat aataactata
     1861 taagttttca attatccaac ctataaaacc atacaagaaa aaattaaata aaaaatataa
     1921 tagagaacct acattatcca tgaatattcc tcctagctgt ttatacttat tttttctcat
     1981 attcatggat ttattctaaa tcaaagaaat tatatttaat ttatttaatt aatactttat
     2041 attatagatt gtgacattct ttagagttta aactttttag gtattctcta acttccagta
     2101 aattttcaag tatcttatgt gattgtgatt taatttgctc atctggctct tttaaatctg
     2161 gtacatttat acatcttata ccaccattgt atgcagcttc gactcccata ggcgaatcct
     2221 ctatgacaat acaatttttg ggattaacat ttatcttttt agctgctttt aggaatatct
     2281 cagggtttgg tttcgagttt acaacatcat ctccacaaac tatagcatca aagtaatctt
     2341 ttaaatttgc cttagctaat ctctttactg ctctttctct ctttgttgat gttgcaacag
     2401 ccattttgta tccattttct tttaaaaaag agattagttc attgactcct aatttgatag
     2461 gagctccctt cctctccata aattcaatca tatttttagt tttttcgtca tataaatcta
     2521 ttattggtac agaactatcg tatatatctg taagaccttc tattattcct ttacgatttc
     2581 ttcccataac tgatgtataa atttccttag tcatagtata tccatacttt tcaaacgtct
     2641 ccatccaaaa ttcaagagaa attctctctg agtcaaacaa aactccatcc atgtcaaaaa
     2701 taattccttc tactttttgc atacaattcc tccaatattt tttgatataa ttatattaca
     2761 ctttttatga ttaattttac aaccctcaat ttatccaatt ctaatattgt aaaattgtaa
     2821 ttttcaaaag ttacactttg gtttacttta atatttgaac caagcatata ggatatccac
     2881 ccacctatac tgtcaaatcc atcatgctct atatcaagtc caaaatactt attaatatag
     2941 ttgatagatt cagttccatc aactaagtaa gaacctgctc ctattttctt aatatgagtc
     3001 tcttctttgt caaattcatc ttgaatttct ccaactatct cttctagtat atcttctatt
     3061 gttaccacac cagatgtacc accatattca tccacaacta tagcaagttg aagtttttcc
     3121 tttcttattc tctcaagagc tttatctata gttaaatttt cagatatata tataatatct
     3181 ctcaatattt cttctagttc aattttattt tcatttatct tttgattgta taaatctctt
     3241 atatgtacaa aacctaatat atcatcttta ttttttctgc atactggata tcttgtaaca
     3301 ccttcctctt ttaaaatagc taatatcttt tcttcagaat cactttcata tatacatacc
     3361 atatcagttc taggaaccat tatctctcta attttctttt cttcaaattc aaaaatatta
     3421 tcaactaatc tttgctctga ttcatctata agtccacttt tataactttc ttctactaat
     3481 agtcttattt cgtcacctgt atggggttca tcaacctcat cagcttgtga atatccaaat
     3541 ggtttaagaa ataaatcagt acttaagttg aaaaaatata ttattggata tgttaactta
     3601 taaaatccta ctaataaaaa agatgtactc aacattattt tttctgtatt ataaagtgct
     3661 aaagctttag gaaccaactc tcctattaca acctccatca ttgttataat taaaaaagaa
     3721 atggaaattg atatagtata tataatactt tcactaagat taaatacact tactattggt
     3781 aaaattaact ctttaactgt agattctccc atccaaccta atgcaagtga acaaagtgtt
     3841 ataccaagtt gacatgcaga aagataggaa tttaagttgt cttttattat tttacatctt
     3901 tttgcattat tattaccttc agctatcatg gtatcaatcc tagaactacg aagttttacc
     3961 atagcaaatt cacaagctac aaaaaaagca tttatcaaaa caagcattac tataagtgtt
     4021 acttttaata actcccgcat aaagtattgg ttcttataat gtaagaaatc tataagaact
     4081 caaagttccc cccttatatt tataaattta atttataata tatattctat catatttatt
     4141 aatatatcct actttattac aaataatttt tataaactaa ataacccctt taatatttat
     4201 gactggctat ttgataaata ttaaaggggt tatttagtta tcatttttta tttctaaaaa
     4261 tcttttctca aatgatttag ttatctattt atccaatttt ttacataatt taatgattaa
     4321 aaatattaat tttttcacta taaagtacaa atattatctg attattaaaa taaatattaa
     4381 tactataaat aaaaacgaag catgattttt atttaaataa atctcaaact tcgtctttac
     4441 ttacttagat atttatttac tttctaaatc ctattatttt taaaacctta tcttttcaat
     4501 acttcaccac atttattctc agtaatctct ccattttcta acgcaatttt tccatttaca
     4561 attacatatt caacaccatt ggctagtaat tgtggctttg aaaaatcaat atcttctgac
     4621 ctaggataac tatagttatc catgtcaata actgctatat cagctatcaa cccttcttta
     4681 agaatacctc tattttttat tttaaattgc ctagctggta atgaagtaac tcttcttaca
     4741 atctcttcca atctaacacc agactttctg tacatattga agaaaattgg aaaagctcct
     4801 cttctttgta gttcaaacca tgacatatcc ccacccacac taaacaaaca atctgaacca
     4861 accataacaa atggattatc aattatttta tcatcatgta gtttatctgg gaaatcagct
     4921 ctatacattc ctcctaaaca aaagataagt tcttcgtctc catctaaaag taaatcgagt
     4981 aataacttgt cttcatctat tcctttttgt gtagctattt cacgtattga tttcttttcc
     5041 atattaatat ctttagtgtt tagaagtatt atactccccc tatctccaag tataaatgct
     5101 tcttttaata taagtgttct tccctcttca tttctaagag catccattac cccatctata
     5161 cctaattcaa atagactaga tgatattgcc ataataaatt gaattactct ttttttcttc
     5221 atacaatgcc cagaacttct tggtactgtg tcaaccatta tttcaacacc attataccta
     5281 gcctttaata tatcatcaaa tgcttctatt tcaactggac ttaagtgaga tacttgaacc
     5341 ctagcaccac ttttttgccc aacctctatg gcttctttta ctgcattata tcttcctatg
     5401 taatctctaa tatgagatgt atatatacca tcatactctt taatctgcct tgcaatatca
     5461 acaagttcat caatatcagc atacttactt ggtatatatg ccaatccagt agatattcca
     5521 aaagcaccac ttttcattcc atcatttatt atatctgtaa tttcattttt ttccttttct
     5581 gtaggaggtc tatccttaga acctcccata acactccatc ttatagtccc atgccccaac
     5641 aagaatccca tattaacaga aataccactt ttagatataa ggtcacaata ttctgtaaaa
     5701 ttattccaac atttattttt atctataaga tacttttttt cttcttcaag taatagacca
     5761 tttttataca tatattcata cacattttca gaagaatatg gtgttataga atgtccacaa
     5821 ttaccattta tagttgttgt aactccttgt tttagaaact tttcaaattt accatctagt
     5881 attgctacta tttcttcatg aacatgaggg tcaataaatc caggtgttat aacttttcca
     5941 caacaatcaa tctctttatt tgtattgcta tttatcttag aatctatttt tgctattaag
     6001 ttgtctttaa ttgctatatc tccataaaaa ccaggattac ctgtaccatc tattataaat
     6061 ccatttttta agattaaatc atatatcaaa atattccctc cttaaaaacc aatttttaaa
     6121 taatcttaat gtaattttta tatttgaaaa tcaaattatt atatttcata tttgaaaatt
     6181 cactattatt tcattgtaat tagtcactaa gcttatcttt atacatagct cttgcaatta
     6241 atatagtacc aaatacacat ataagaacta ctagagactg tggtattata ccttgctttg
     6301 ataatataat agtcaataca gatattacaa ttgctactag acctaatttt ttatcttgta
     6361 aaaatacttg tccaaaaact gacccaaata gagctggtaa aattaagttt aatttttcta
     6421 ctacttcagc tggaatttta gataatacgc ttcctcctaa aattacccct atagttagta
     6481 tacttatatt cactaaaata gatacagcta ttcctattgt agatattatc gagccttctt
     6541 ctgtacctga ttcaacttct gctgcctttt gagctgctat tgaacatggt agcctcatat
     6601 tagatatatt accagaaaga aaactcatat aagttcctgg gatacctaag attggtgaat
     6661 aagaaactgg ttctattatg taatttggag cactcatact gacaattgcc attgtacttg
     6721 ctaataatgc tgatattgga ggcataatac caaatccaaa gctcaaaatt attccaggaa
     6781 ctaatgctgc tataataccc aaagatagtg ttagtcttcc ataccttatc ataaaaggta
     6841 aatattcttc attgtatata gattttactt tactcataaa actcccccct tatattactg
     6901 aagctattat cattccgcta aacattgata ttgtaagccc ccactctctt aaccatttta
     6961 tatttttctt cttttcaagc atacacaata ttaccattat agccattcca cttatacatg
     7021 caattgttcc attatcaaaa cgcaacaccc tatcagcatt tagatatgca aatgaaccca
     7081 acattgctcc tagagatatt attggtataa atgatttctt gccactagat ataacattgg
     7141 ttatcttatc tagtttatgt ccaaatagta atgtgaatat tatccatcct aaagaaccta
     7201 atatcatagt ccaaactgca tttgtaaatg caagttttgt catttcagga gaacctaaag
     7261 ttatacctaa tgcatctgtt ccaaatccgg ctgacattga ttcaaaaatt acagaaccta
     7321 taaatgataa tctcatccat gctataggcc cacccatagt tactataagc gataccattc
     7381 cagcaaatat agtcattgaa ggcccaatag ctgaaatagc actactctta acagctgact
     7441 ttatttgttt atctgttatt cccattgttt taccaacttt gcaagatttt atagcaaata
     7501 taagggattg taccactact attgatactg ctactgtact cgctatccac aaaagtggat
     7561 gattagctaa ttttaaataa tccatttata aaacctccct ataaatcctc aaattttatt
     7621 aatatttcta atatatctat ttccaaacct gttttagtac tctaagtata tttccaccta
     7681 gtaccttctc tatttcctca tcactatatc catgctttac taaatatcta acaatgtttt
     7741 ttgaagtttc tgttgggttc tcaagacctt ctacatattc tactttggga tgttcttttg
     7801 ttcctgttat tttttttata tcaaatttag cagctagtac tgagtgtaat tttacatggt
     7861 cgccatatac tgtatctggt ccaaaagcta catggtctat tcctactaaa tttttaatat
     7921 actcaaaatg ttccataaat gactctattg aatgttcttt attcttctct gttattgtag
     7981 tatgaggtgc tgcttctaca cctataaccc ctcctttttt agcacatgct attaatactt
     8041 catctggagt tagtctcttt gtgttccata aacttcttgc cccaacatgg cttaatatta
     8101 ttgggtcctt gctatattct attacatcaa gtgtagtttg aactccaacg tgagagcaat
     8161 ctattgccat tccaatttta ttcattcttt caacagcttt tttcccaaaa tttgtaagac
     8221 ctccatcttt ctcttctttt agtcctgaac ctaatgcatt ggattcgctg tatgttatac
     8281 ccattaacct tataccaaat ccatacagta tatctattct gtcaagttca ttttctattg
     8341 gtgctgcacc ttctaatgtt ggtatcaatg ctactttacc ttcactttta gctctgtata
     8401 tatcttctac tttttcacac tttatgacaa aatcttgatg agctaaatca catagtctca
     8461 tgcctaaatc tattaatata tcatcccatt tccaccctga atttgatgtt atcgtacata
     8521 caccatccat taaattatca aatatagcat cataatatcc tcttgataat gcttcataag
     8581 cgcatctttg tttaccttcc ctattatatt caaatatatc tctttctagg tgttctggaa
     8641 ataatactgg atgttcatgt aatgatataa atattttttc atccactata cttttaacct
     8701 tcgcttcttg aatatcatct aattcaataa ggtactcttt aactctttta tctcctttac
     8761 acatctcaat agatgaaata tcttttcctt tttctaaata actataggct tcatatcctt
     8821 tgtaagctga cttcataaca acatcctcca agttctacaa ttttttatat ttttcctatg
     8881 tagcaattga tgtgccaaaa tataaatttt aattgtataa taaatcttgc aaaaaataaa
     8941 aacagtagta aggttgatat ttactactgt tttattaaaa ataatcttct tagattattt
     9001 attcaatatt tttgaattat cttaatctta aagataattt tatttatttc tatccattat
     9061 agggattttt ttagggtgtt ttgccttttt agtcccctat atcactccct taatttttac
     9121 atatgatata aagttctttc cttcatcagt tataaaacta ccttgttttt ttactccaga
     9181 taaaagtaaa ccatggctct ttaacttatc tattcttttt ctaatttggt ctacacttaa
     9241 aactatatct ttttctttta aaatctcttg cagtttattt cttcctaata atatattttt
     9301 attattccaa gtttctatac tggtcaatat acatatactt tcctcaaaga aattagattg
     9361 tttgaaatct aagataattg aatcgaaatt ttcattaact aaagtattgt ttttttcaaa
     9421 tctaaattgt tctggcaaat gctcgtaatc tactctatct tcctcaacta tagtatcaat
     9481 ataataaatt aaatttttaa gctctcttac atttcctggc cattcataca atgttaaagc
     9541 ttgcattgat tcctctgtaa aacatttgtt gctatttatt tcatctaaaa aatacttact
     9601 tataagaggt atatcttcct tacgttctct aagtctaggt atctctatat gtaatacatt
     9661 aatcctataa tacaaatctt ctctaaagga accttcttta atttttttct ttaaatcttt
     9721 atttgttgca gctataattc taacatctat tggtataatt ttacttccgc ccattctcat
     9781 aacttctttt tcctgtaata ctcttagcaa tctctgctgt acatctaaag atatatcacc
     9841 tatctcatct aaaaaaattg ttcctgtgtg tgctctttca aatataccca ttttcccacc
     9901 ttttattgct cctgtaaaac ttccttcatc atagccaaaa agttcacttt caataagtgt
     9961 atctgacaaa gaagatagat taactgcaac aaatggcttg ttttttctta gagattcatt
    10021 atgtattgcc tgggcaaata tttcttttcc tgttccattt tctccaagta tcaacacaga
    10081 aaagtctgta gttgctattt ttcttgcaat attaattttt tcttttatta ttttactctc
    10141 acctacaaca ctctcaaatg tatactttga tacaaaacct tttgactgaa gtttattttg
    10201 aatcttgtcc tcaagctctt gaattgcact tatattttta atgcttataa tactctttat
    10261 tcgttcatta ttttcataaa catttacttt attaattatc agttttttgt tatttaaatt
    10321 tactacttca tcattaactt catcttcttg aaaaaatatt tttttgacaa ccttatcact
    10381 aaataagtcc ataaagttat tagatataat ttcattttgg tctattccaa ctagattaga
    10441 aaatactttg ttgcaatata taaattttcc taatttatca acagaagcaa taccttcatc
    10501 tataatctcc aaaaaactct tcatggtttt attcatagtt gtataatacc tatatccact
    10561 tactgtatct tcattgtatt tttccttaat aatatgtaat ttatctatag atatatttag
    10621 ttttgtaaag atttctataa ctgtatttat atcaattacc ttatcaccta tgtcaattat
    10681 ttgctttatg ttttgtggta tgctgtttct gcttcctgta ataattccaa tctcacattt
    10741 agacttatca caacctggat aatatggtat tagattaata tgaattaatc ctagttttct
    10801 aattatcttg gcagtttcat ctgctgactc tttataagca tctataacca taacatcaga
    10861 atcattttct atacttatta tctgatttat attttcaata tttatagtac gatgtactat
    10921 tacaataggt atatttttat caatattatt atgtatatat tcctttatat cattgtctga
    10981 acatactaca agctcatagc ttgacagttc aatatttatc ctatttacat ataccaagtt
    11041 atctatatta caaaattctc caaatatatt ttccaattgt tttgatatat ctatatttat
    11101 tttgtcatca gttgatacaa ataaaatatt atgttttttc atagtcccac ctcctgttta
    11161 acgagtatag aaagtaataa tttggtctat tgtagcactt tttaaaaaag ttttaaatct
    11221 taaaaatcaa attttgtacc atttactatt attttccata ataaaataat gtaagctatt
    11281 tttttcacat ataatttaat ttctaaaacg gttagtttga cattattgga atataaacaa
    11341 aaatttaata taaattcgtt aatatattaa tacattttta agaatgataa ggaataaatt
    11401 ataacaaatt ttaggaatat ataataatag ggtctcagaa cttagttaca agttctaaaa
    11461 ccctatttgt ttagtgtaaa aaacatcata aaatttagct taaatctttt cacataatat
    11521 tactcttttt ctaagtgata ataaaatata tctattcctc taaaaactgt aaaaattcct
    11581 gattaaatag tttaagctca tcatagaata tagcatgacc actatactca aaagtatata
    11641 aaattgaatc ttctattttt tcattcataa aaactgcaaa ttcatacgga catatttcat
    11701 ctaatttccc atgaaatata ccagtaggca ctcttacaca ttttaaatca ttaaataatt
    11761 cttcatctct taaagataca gcagttccaa tagtgccaat acctgatgca ctaaaaccaa
    11821 tatcattaaa ccatcttcta aagctttctg gtggatttga tgcaaatact ttttctccaa
    11881 aatcggttac catttctggt ctatcagtac aggcttgtgc tatcaattta tttacatctt
    11941 cacgtgtcat accatatgga aattctggag gtctttgtac aaatgatggt gcagcagctg
    12001 cagctaatac caattttgac actccatatc cattaaataa actcatatac ctaagtacga
    12061 ttgctcctcc cattgaaaaa ccaacaaggg taaagtcttt taatccaata gcatgtacca
    12121 ccttataaat atcgtctgct aattggctat aagtatatcc tcctgatgtt gcatcagatt
    12181 taccaaaacc tcttaaatca attgaaactg ttctatatcc gagttttggc aatatatttg
    12241 tttgatattc aaatatctta tgtcctaagg gccaaccatg tataaaaagt acagtttttt
    12301 ttagcactcg gattcaaatc atatactgct atgtttatat cattcacata tacataatac
    12361 atatcaacag caactccctt caagaaaatg tatgatggtt attttatata tatgaataaa
    12421 atgatgaata actaaatttt caaatttatt tacaatactt ctctattgcc ttttcaaata
    12481 aacttacatg aagtttttca tctaggatta ttctatttaa tatagctttt atataagggt
    12541 cttcaataag acttatatct ttataataat tattaatagc cacatgctcc aaatgcaaat
    12601 ctagttttaa tctgttacaa atagaatttc cataacatac aaaaccacca ttccaatatg
    12661 caccacaaga acttgtagaa cctctatata caggatttcc acccaataat cttatagtct
    12721 ttgctaatat ctccatatgt aacatttctg ttatagatat atttatccac atttcactta
    12781 actctctatg cgtttcatct aaatcaaaat tatggtatag atattgattt acactcgtaa
    12841 attcacttga tactccggca tagtcatcca tcaataattc tgcatagtac ttatttggac
    12901 ctaaaacttt tatctctgga tatggctcat cacttgaata tccatttcgc ttgtgcatgt
    12961 cataatgtgc atcactcata atatctcctc ctttaaattt aatgtatgat attaattgta
    13021 tgcatcacga ctttttcata taacttagat aataaaatct acttaaataa taaaaagcca
    13081 tattaattaa aattaacatg accttttatc cacaaaatta ctttttaatt attcatcttt
    13141 tccagctact gatagctctt taataacaac tgaaggcgaa cctatactac tcattggaaa
    13201 ttctaaatct gctcctatag cttcaatatc ttttaacagg tcaaaatagt ttcctgctac
    13261 tgtaatttgc tctactggaa atactttttt tccttctttg atataaaagc ctttggcagc
    13321 taaagaaaag tctcctgtta ctgaatttgc acctgaatga agtcctgcaa agctagtaac
    13381 catcaatcct tcatctatat cctttataat ttcatctaaa gatttatctc ctttctctat
    13441 ataaaaatta gttggatata tgcttatagg tgatgaatat gaagatttta ttccatttcc
    13501 tgtagtcttt acacctgcct tattagccgt ttttaagtta tgaagtaaag ttataagctt
    13561 tccatttaaa acaacttctt ttttaaatgt agctactcct tcatcatcaa aaggagtaga
    13621 tgccatacca ttttctaaaa gtgggtcatc aactattgta actataggag atgctatcat
    13681 gtctccttct ctatttttca ataaagataa tcctttttga gctgaatctg cattaaatat
    13741 cccagaaaat gtgctaagca aagataccat agcttcatta agcaatatag ttttatattt
    13801 acctgatgga atactcttac cacctacttt agataaagca ttatcaacac caagttttgc
    13861 tatctcacaa ggttttattt cttcaataga atttgctata ttatatccta ttccatcata
    13921 tttttgtcca ttgtcttcta tgataggaac tacaaaccct ataagcatat tagttttatt
    13981 acttaaatta agacctttag tattagatat accattactt gatacagaat aagatatagt
    14041 acatttagat aaatttacca ccttatctga ataagcttta gtctctcttt ccatttctaa
    14101 agctaaatca atcaacttat ctgcctctaa attttctagc tctttagaat aagtctttac
    14161 atcattatag tgcttatctc cttcatatat aaattgtaca tcttcatttt caatagtgtt
    14221 tactccatct tttacatttt ttatcaacat atcaacagct ttatcatcta aaatttctgt
    14281 ataagaataa cctatctttc catttacctt acctctaaaa gataaaccaa aagatttatc
    14341 aagattgtat ttctcaactt ctccttcata tatatttata cttaaatttt ctcctgtata
    14401 ataatatatt tcacactctt caaaaccttc acttagagcc tttttaaata atatatcttt
    14461 aaaattttta agttccacaa ttatccctcc tatcttccac ctacagtaat ttcttttacc
    14521 cttatcatag gttggcctac attagttggt atacttcctg atgatgagcc acacatcccc
    14581 tgagcttgtt ttacgttatt cccaaccata tctatattca taagtatatc gctacctttt
    14641 ccaattaaac tagcacctct aactggctct tgtatttctc cattttttat taagtaacct
    14701 tctgaaacta caaaattaaa ctctcctgtt acaggattta cagaaccacc acccattttt
    14761 ttagcataaa gtccatctgg tattgacttt ataatatctt ctggtttatc ctctccagca
    14821 gcaatataag tgttagtcat ccttgaagtt ggtgcaaatt tgtagttctg tctacgagaa
    14881 cttcctgtag gattcattcc cattcgtcta ccattaagtt tatctatcat ataagacttc
    14941 aaaattccat tttcaatcag tatattcttt tgagttggat ttccttcatc atctatattc
    15001 atagaacccc agtagtttgg tattgtacca tcatctatag ctgtaatctt tgtggaagca
    15061 atctgttgac ctaatttatc tgcaaataca gagttcccct ttgcaactga tgtagcttcc
    15121 aaagaatgtc cacaagcttc atgaaatatt actccaccaa atccattatc tattgctacc
    15181 atcatattac ctgcaggaca atttttagca tgcaacatag tatgcgctac tctagctgat
    15241 tcttttgcat aatattctgg gtctatttct tcaaatattt caaacccttt acaacctcca
    15301 ggaccttcaa atccagtttg attttcatta cccttagatg caattgaact tatccctagt
    15361 ctagttctta ttcttctatc ctcaacatat ataccttcag tgtttgcaat taatattttt
    15421 tggtctttat ccaaataact aacacttact tgagatatat cattactaaa ttcttttgca
    15481 ctcttatatg caaccttcat aacacttact ttatctttat ttcctattga atttggataa
    15541 atttttatat tatgtatgtt atttatttta atactgtcat ttaatattac actttttcca
    15601 tcttccaatt tacctaaagc aacagcagct ttgtatgcta catccaacag actatttaat
    15661 gaattgtcac ttgtataagc atatatactt ttcaatcctt taaatattct tattcctata
    15721 ccataatttc ttccacctat tgcattttct actttattat ctattaaact aatagaatta
    15781 tttatagtgt cttcttcaaa aatctcagca aaatctcctc cagtaattag acatttttct
    15841 aataccttag aagctatatt ttttgaaagc ataaatttac ctcctacttt aaatttttaa
    15901 ttaactttta ttcaatcttt ttacttgtta acaaacatta tatactatat tacttaaaat
    15961 aaggtaatta tgaatatatt atacatatgt tttatactaa aatgttatca cattttatct
    16021 atacataccc atataactag ataattaaaa tgtataatat tatttgctat agaaaataat
    16081 acactcatac ataataaatt ttaggagtga agtatatgaa tcaagaatat gtaaaaggaa
    16141 atcctatttt tactaatatt aataaagttc caagacaatt tccatacctt actgatgata
    16201 ttgatactga tgttataatt gttggtggtg gcgtaactgg atgtatttgt gcttactatc
    16261 tagctaaaaa caatataaag tctgtcattc ttgaaaaagg aaggattgca catggaagca
    16321 ctagcgtgac aacttctcta ttacagtatg aattagatga caatttgata gatttaacag
    16381 aggttatgac tctaaaagat gctctaaaag catacaacct ttgtacttca gcacttgaag
    16441 aattagatac atttatagag ctatatggaa ataagtgtga ttatgccaaa agagatactc
    16501 tactttatac tgccaacaaa cttgaagtta aggctatcaa agaagaatat aacttaagaa
    16561 aagaaaatgg atttgatgtt gaatatattg atgaatccac aaaccctttt agctttgatt
    16621 taaaatcagg attgatagca aagaatggag gacgagaatt agacccatat aaatatagtc
    16681 accatttgat tgatgtaagt ttaaaaaatg gtcttcaagt atatgaaaat acagaagtaa
    16741 aaaaagtaga tttatcaaat gataaggtta ctgctgaagt atcgtatgga tacaaagttc
    16801 atggtaaaaa attaattgta gctacagggt acaatacaag tttatttaca aaaagaaatt
    16861 ttgcaactaa gtcaaatact tttaatatag caacgaaacc acttaaaaac atagcttctt
    16921 ggaaaaacaa cattttaatt agagataact gtgacccata taattatcta agaactacta
    16981 aagacaatcg attaattata ggtggtgaag atgtaagttt tgatgatata gaaaatgaaa
    17041 ctttagccaa tgaaaagtat gatattttag aacaaagatt gaaaagtatg tttaaggata
    17101 taaaagacat tgaagttgaa tataaatatt gtggttgttt tgcttcaact ttagataatt
    17161 taggttttat tggtccagat aataaaaata acaatctatg gtattgctta ggttatggag
    17221 caaatggtat actctttgca atattaggag gtataatgtt aagtgaatta tatcttggca
    17281 aacaaaataa aaatatgaaa ttatttaaag tagataga
//
LOCUS       DAKBNN010000072        17140 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000072 DAKBNN010000000
VERSION     DAKBNN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.072"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            520..1719
                     /gene="hadA"
                     /locus_tag="OUB02_001991"
     CDS             520..1719
                     /gene="hadA"
                     /locus_tag="OUB02_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HCT8885748.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1758..2558
                     /gene="hadI"
                     /locus_tag="OUB02_001992"
     CDS             1758..2558
                     /gene="hadI"
                     /locus_tag="OUB02_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HCT8885749.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2573..3799
                     /gene="hadB"
                     /locus_tag="OUB02_001993"
     CDS             2573..3799
                     /gene="hadB"
                     /locus_tag="OUB02_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HCT8885750.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3799..4926
                     /gene="hadC"
                     /locus_tag="OUB02_001994"
     CDS             3799..4926
                     /gene="hadC"
                     /locus_tag="OUB02_001994"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HCT8885751.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4974..6107
                     /locus_tag="OUB02_001995"
     CDS             4974..6107
                     /locus_tag="OUB02_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCT8885752.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6140..6925
                     /locus_tag="OUB02_001996"
     CDS             6140..6925
                     /locus_tag="OUB02_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCT8885753.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6944..7981
                     /locus_tag="OUB02_001997"
     CDS             6944..7981
                     /locus_tag="OUB02_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCT8885754.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8464..10095
                     /locus_tag="OUB02_001998"
     CDS             8464..10095
                     /locus_tag="OUB02_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885755.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10230..11156)
                     /gene="fba"
                     /locus_tag="OUB02_001999"
     CDS             complement(10230..11156)
                     /gene="fba"
                     /locus_tag="OUB02_001999"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HCT8885756.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11486..11815
                     /locus_tag="OUB02_002000"
     CDS             11486..11815
                     /locus_tag="OUB02_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HCT8885757.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            11815..12537
                     /locus_tag="OUB02_002001"
     CDS             11815..12537
                     /locus_tag="OUB02_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCT8885758.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPAGYISATTDKYDPTVLDLIDLSYLVFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12564..13925
                     /gene="rlmD"
                     /locus_tag="OUB02_002002"
     CDS             12564..13925
                     /gene="rlmD"
                     /locus_tag="OUB02_002002"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCT8885759.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            14436..15470
                     /locus_tag="OUB02_002003"
     CDS             14436..15470
                     /locus_tag="OUB02_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HCT8885760.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGIAAVVAGV
                     VYKKKKRKKI"
     gene            15557..15760
                     /locus_tag="OUB02_002004"
     CDS             15557..15760
                     /locus_tag="OUB02_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCT8885761.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15931..16818
                     /locus_tag="OUB02_002005"
     CDS             15931..16818
                     /locus_tag="OUB02_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HCT8885762.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIK"
BASE COUNT         6829 a         1949 c         3271 g         5091 t
ORIGIN      
        1 ttatttaatg taataattac tctaaaattt atcaaagaaa ttgttatttt ttagattaaa
       61 tattattaaa aaattatcta agtttgaaat tagttaacta acttgctgat taattacaat
      121 gataacatgt gtttttgagt ttagcaatga aaactttttt tgaaatgtca agctttttca
      181 taaaaaaata tataaaaaaa acttttataa gcagagaaag aatagcaaaa tgcaatggat
      241 agtggcaaga tgccacgata tgaaaaagat tattcaattg catattgtta aaaaacattc
      301 aaatcaagcg atattttgat taaatataaa gttggcacga taaatgctta acaatatagg
      361 tgtaagacaa taaaacaata aaaaataatt ttaataattt ttaagttatt ttagataaga
      421 ttaggaactt tgacatcaga cagttaatat cagaaaaaag tattttatga gtgatttaat
      481 cgaaatttta taattgatac tattaaggag gaaaacgtta tgcttttaga aggagttaaa
      541 gtagtagaac tttcaagttt catcgcagca ccatgttgtg caaaaatgtt aggtgactgg
      601 ggtgcagagg ttattaagat tgaacctata gaaggtgatg gaataagagt tatgggtgga
      661 acatttaaat ctccagcatc agatgatgaa aaccctatgt ttgaattaga aaatggaaat
      721 aaaaagggtg taagtattaa tgtaaaatca aaagaaggag tagaaatatt acataaatta
      781 ttatcagaag cagacatatt tgtaactaat gttagagttc aagcattaga aaaaatgggt
      841 atagcttatg accaaataaa agataagtat ccaggattaa tattctctca aatattagga
      901 tatggtgaaa aaggaccttt aaaagataaa ccaggatttg actatactgc atacttcgca
      961 agaggaggag ttagccaatc tgttatggaa aaaggaacat ctccagcaaa tacagcagca
     1021 ggatttggtg accactatgc aggtctagca ctagcagcag gaagtttagc agcattacat
     1081 aaaaaagctc aaactggtaa aggtgagaga gtaacagtaa gtcttttcca tacagctata
     1141 tatggaatgg gaacaatgat aacaacagca caatacggaa atgaaatgcc tttatcaaga
     1201 gaaaatccaa acagcccatt aatgactaca tataaatgta aagatggaag atggattcaa
     1261 ttagctttaa tacaatacaa caagtggtta ggcaaattct gtaaggttat aaatagagaa
     1321 tatatattag aagacgatag atataataac atagattcaa tggttaatca tgttgaagat
     1381 ttagttaaga tagttggaga agctatgtta gaaaaaacat tagacgagtg gtcagcttta
     1441 ttagaagaag cagacttacc atttgaaaaa attcaaagct gtgaagattt attagatgac
     1501 gaacaagctt gggcaaatga cttcttattt aagaaaacat acgatagcgg aaatacaggt
     1561 gtcttagtta atactccagt tatgtttaga aatgaaggaa ttaaagaata tacaccagca
     1621 ccaaaagtag gtcaacatac tgtagaagta ttaaaatctt taggctacga tgaagagaaa
     1681 ataaataact ttaaagatag taaagttgta agatattaat tagtaattct agatttttta
     1741 aaaggagaat gctaaagatg tacacaatgg gattagatat aggttcaact gcatcaaagg
     1801 gagtaatctt aaagaatggg gaagatattg tagcttctga aacaatatcc tctggtactg
     1861 ggactactgg accatcaaga gttttagaaa aattatatgg caagacaggt cttgcaagag
     1921 aagatattaa aaaagttgta gttacaggat atggaagaat gaactattca gatgctgata
     1981 agcaaataag tgaattaagc tgtcatgcta gaggggtaaa tttcataatt ccagagacaa
     2041 gaaccattat tgacataggt ggtcaagatg caaaggtatt aaaattagat aataatggaa
     2101 gactattaaa ctttcttatg aatgacaaat gtgctgcagg tacaggaaga tttttagatg
     2161 taatggcaaa aataatagag gttgatgtat ctgaactcgg aagtatatct atgaattctc
     2221 aaaatgaagt atcaataagc agtacatgta cagtatttgc agagtctgag gttatatcac
     2281 atttatctga aaatgcaaaa attgaagata tagtggcagg tattcatact tcagtagcaa
     2341 agagagtttc tagcctagta aaaagaatag gagtacaaag aaatgtagtt atggttggtg
     2401 gagttgctag aaatagtggt attgtaagag ctatggcaag agaaatcaac acagaaatta
     2461 ttgtacctga tatacctcaa ttaactggtg ctttaggagc agcgttatat gcttttgatg
     2521 aagcaaaaga atcacaaaaa gaagtgaaaa atatataaaa agagggatga aaatgtctga
     2581 aaaaaaagaa gctagagtag taattaatga tttattagct gaacaatatg caaatgcatt
     2641 taaagctaaa gaagaaggaa gacctgtagg ttggtcaaca tcagtatttc ctcaagagtt
     2701 agcagaagta tttgacttaa acgtattata tccagaaaac caagcagctg gagtagcagc
     2761 taaaaaaggt tctttagaat tatgtgaaat agctgaatct aaaggatatt ctattgacct
     2821 atgtgcatat gcaagaacaa attttggtct tttagaaaat ggtggatgtg aagctttgga
     2881 tatgccagct ccagatttcc tactttgctg taacaatata tgtaaccaag ttataaaatg
     2941 gtatgaaaat atttcaagag aattagatat acctttaata atgattgata caactttcaa
     3001 taatgaagac gaagttactc aatcaagaat agattatatt aaagctcaat ttgaagaagc
     3061 tataaaacaa ctagaaatta tatcaggaaa gaaatttgac cctaagaagt ttgaagaagt
     3121 aatgaaaata tcagctgaaa acggaagact atggaagtat tctatgagtt taccagcaga
     3181 ttcttctcct tctccaatga atggatttga cttatttact tacatggctg taatagtttg
     3241 tgctagaggt aaaaaagaaa ctacagaagc atttaagtta cttatagaag aattagagga
     3301 caacatgaaa actggtaaat cttctttcag aggggaagaa aaatacagaa taatgatgga
     3361 aggtatacct tgttggccat atataggata caagatgaaa acattagcta aatttggagt
     3421 taacatgaca ggtagtgttt acccacatgc ttgggcatta caatatgaag ttaatgattt
     3481 agatggaatg gcagtagcat atagtactat gtttaacaat gtaaacctag accgtatgac
     3541 aaaatataga gttgattctt tagtagaggg taaatgtgat ggagcattct atcatatgaa
     3601 cagaagctgt aaacttatga gtttaataca atatgaaatg caaagaagag cagctgaaga
     3661 aactggatta ccatatgctg gatttgatgg tgaccaagca gaccctagag ctttcactaa
     3721 tgctcaattt gaaacaagaa ttcaaggttt agttgaagta atggaagaaa gaaaaaaact
     3781 taatagaggt gagatataat ggaagctatt ttatctaaaa tgaaagaagt agttgaaaat
     3841 ccaaatgcgg ctgtaaaaaa atataaaagt gaaactggta aaaaagctat aggttgtttc
     3901 ccagtttatt gcccagaaga aattatacat gcagctggaa tgcttccagt tggtatatgg
     3961 ggaggacaaa cagaattaga tttagctaaa caatatttcc ctgcatttgc atgttcaata
     4021 atgcaatcat gtttagaata tggattaaaa ggtgcttatg atgaattatc tggagttatt
     4081 ataccaggta tgtgtgatac actaatttgt ttaggacaaa actggaaatc agcagtacct
     4141 catataaaat atatatcatt agtacaccca caaaatagaa aacttgaagc tggtgtaaaa
     4201 tacttaatca gtgagtacaa aggcgtaaaa agagaacttg aagaaatttg tggatatgaa
     4261 atagaagaag caaaaattca tgaaagtata gaagtttaca atgaacatag aaaaactatg
     4321 agagactttg ttgaagtagc ttataaacat tctaatacta taaaaccatc aataagaagc
     4381 ttagtaatta agagtgggtt ctttatgaga aaagaagaac atactgagct agtgaaagat
     4441 ttaatagcaa aattaaatgc tatgccagaa gaagtctgtt ctggaaagaa agttttatta
     4501 acaggtatat tagctgattc taaagatata ttagacattt tagaagacaa caatatatca
     4561 gttgtagctg acgacttagc acaagaaaca agacaattca gaacagatgt accagcaggt
     4621 gatgatgcgt tagagagatt agcaagacaa tggtcaaaca tagaaggatg ttcattagct
     4681 tatgacccta agaaaaaacg tgggtcactt atagtagatg aagttaaaaa gaaagatata
     4741 gatggtgtta tcttctgtat gatgaaattc tgtgacccag aagaatacga ttatccttta
     4801 gttagaaaag atatagaaga tagtggaata cctactttat atgttgaaat cgaccaacaa
     4861 actcagaata atgaacaagc cagaactcgt attcaaactt ttgctgagat gatgagttta
     4921 gcgtaagttc attaaaataa atttactata aaatatgaaa attggaggaa gctatgttat
     4981 ataacaaaga acaagaactt ttaagaaaag cagtaagaga ttttgttagt aaagaattag
     5041 atactttacc agcagaaatg gataaaacag gtgtaatgcc taaggaatta atcaaaaaat
     5101 tagctgatgc aaaatttata agcagtaata tcccagaaga atatggtggt gggggagcag
     5161 gatatgtttc ttacgctata gtaatggaag aaatagctag aagatgtgct tcaacagcta
     5221 cttttgttac agctggttct tctcttgctt cattaccaat actttacaat ggtactgaag
     5281 agcaaaaaca aaaatactta aaaggtatag caacaggtga attaatagga gcatttggtt
     5341 taactgagcc aggagctggt tctgatgcag gtggacaaca aacaacagca gaattagttg
     5401 gagaccacta tatattaaat ggtagaaaaa ctttcataac taatggacca ttctgtgatg
     5461 tagctatagt aatagcagtt acagatagaa gtaaaggact tagaggaaca tcagcgttta
     5521 tagtagaaag taaatgggat ggtttctcaa caggagctca tgaagataag atgggtataa
     5581 gaggaactga aacttctgac ttaatatttg aaaacgttaa agttccaaaa gaaaacttac
     5641 ttggaaaaga aggtcaagga tttaagatag caatgggtac tctagaagtt ggtagaatag
     5701 gtgttgctgc cttagctcta ggaatagctc aaggtgcttt agatgaagct gttaaatata
     5761 caaaacaaag agttcaattt ggtaagccta tagctaaatt ccaaaatact caatttacta
     5821 tagctgacat ggaaacaaaa gtttgtgcag ctagaggatt agtttatgat gcagcacaaa
     5881 agagagatgc aggaatgaga gttgctcaag aatctgctat ggctaaatac tatgcatcag
     5941 aaattgcaaa tgaagttgct tataaagcat tacaacttca cggtggatat ggatttataa
     6001 aagattatga aatcgaaaga atgtacagag atgctagaat cgtatcaata tacgaaggaa
     6061 catcagaagt tcaaaaaatg gtaatttcat caaacgtatt aaaataataa ataatctaat
     6121 gaaattggag gtatagactt tgaaaatatt agtttgcgta aaacaagttc cagatactaa
     6181 cgaagtaaga ataaataaag aaacaggtac tcttataaga gatggagttc caagtatatt
     6241 aaacccagat gatgcaaatg cattagagga agctttaaaa attaaagatg agcatgatga
     6301 tgtaactata acagttataa caatgggacc tcctcaagca gactttatgc taagagagtg
     6361 tttagctatg ggtgctgatg atgcaatatt attaagtgat agagcatttg gaggagctga
     6421 tacttgggct acatcaaata ctattgcagc gggaatatct aaagtaggtg actatgacat
     6481 aatatttgca ggaagacaag caatagatgg agatactgca caagttggac cacaaattgc
     6541 agagaaatta gacattcctc aagttacata tgtacaagat ttcaaaatag aaggaaaaga
     6601 tataatagtt caaagacaat tagaagatgg atacgaatta ataaaagtaa gtacacctgt
     6661 acttttaaca gcagtaaaag agttaaatac acctagatac atgtctgttg ataaaatagt
     6721 aaaagcatat aaacatgatg taaaagtttg gacaattgat gatttagatg taaataaaga
     6781 agaagttggt ttaaaagcat caccaactaa agtatttaga tcatttactc ctgaaccaag
     6841 aggaaaagga gaaatcttag aaggtaaagc tgatgaaata gctagtaaaa taataatcgg
     6901 tctaaaacaa aagcacatta tataagttgg aggagtacaa actatgaatg atataaaaga
     6961 tttaagttct tataaaaacg tatggatatt tgcagaacaa agagaaggaa aaatagctcc
     7021 agtagttata gaattattag gagaaggaag aaaattagct aaagaagtag atgcagaact
     7081 ttgtgcaata ttattaggaa aagatgttga tggattagct aaagaattaa tcacttttgg
     7141 agctgacaaa gtttatgttg cagatgatgc tcttttagaa aaatatacaa ctgatgcata
     7201 tacaaaagta ataaaagatg caatagatga aataaaacca gaaataatgc ttttcggagc
     7261 aactcatata ggtagagact tagcacctag aatagcttca agagttggaa ctggattaac
     7321 agctgactgt actaagttag aaatagaccc agaagataag aaaataaaac aaactcgtcc
     7381 agcatttggt ggaaacataa tggctacaat catttgtcca aaccatagac ctcaaatgtc
     7441 tacagttaga cctggtgtta tggataaagc tgaaaaagac gaaacaagaa ctggtgaagt
     7501 tatagcatta gactacaaaa taactcaaga tgatataaga actactgttt tagaaacagt
     7561 taaaactaag aaagatttag tatctcttac agatgcaaat gttatagtat caggtggttt
     7621 aggattaggt ggaccagaag gatttgaaat gcttaagaaa ttagctgaca aattaggtgg
     7681 agtagttggt tcttctcgtg cggctgttga tgctggatgg atagaccatt ctcaccaagt
     7741 aggtcaaaca ggaactactg ttaaaccaaa cctatatata gcttgtggta tatcaggagc
     7801 aatacaacat ttagcaggta tgcaatcatc agatttcata attgctataa acaaaaaccc
     7861 agcagctcca attttagaaa tcgctgacta tggagtagtt ggagacttac atgaaatagt
     7921 tccaatgctt atagaaaaat tagatagtgt tgatgattta ttagaagcta taaaagctta
     7981 atagatacaa actatataaa tattactgat tgaataagtc ttattagtta gtaacataat
     8041 tattaaccct ttgataagtt attgctagtg tgtatttgtc agttattaaa aagtctgttt
     8101 gaataaaatt ttataataca agataatctg gataatcctt atatgtatat tgcttacaaa
     8161 ttgtgagtta tattctgtaa ggattatctt tgttttttta aataaatctt aaatttaatt
     8221 aaattgtatt aaatagacaa ataattacta aatatgtgat aaaatacaat taaaagcaaa
     8281 atgaaatttt taacaaacta taatcattaa aattatatct tagttacgtt ttaatgaaat
     8341 aaactacttg aaaagacgaa tattataagg agttgattat atgtgctgat ggtatttatt
     8401 gtaagcaaac aataaatttt gtatgtagtt caataaatta cttaatgaaa ggtgcgaata
     8461 ttaatggata ataatttaat agggcttata gaacatgtag aaaatccagc cattttatgt
     8521 aaagaatcag gcgaaataat atactgtaat catctaatag atagtatttt tagttttcta
     8581 gatataaaaa agcctagaaa tataaatgaa ttagattcaa attttgacaa aacagaaata
     8641 ctgacagata gtaaaaagaa aatagctttc agagagttaa ggatgactgc acatatatac
     8701 aatatgaaag ataataataa tgaaaataat atagtttatt tatttgagaa gtctctaata
     8761 tctgataaag taattgaaga tataatagag catatagatg aagttgttgt tgtgtttaac
     8821 aaagatgggg taattgaaaa gatgaacact gtcagtgatg agatattacc ttttaaaaga
     8881 actgaggtac ttggtagaaa tataacagac cttgtgagac aaggattagt tgaagaacca
     8941 ataatattaa acatgcttaa agtgaagaaa aagatttata gaaatatcgt ttacccagat
     9001 gggaagctta ttgcatatac ggcagttcca agatgggatt ctaagggaaa gcttacagga
     9061 ggagtactaa cagggagaga tatttcaagg gttattaagc ttgaatctca gataaaatat
     9121 agtgatatat cagaagacac agagtatata agtcaaagta agattatgga caatataaaa
     9181 aaagttgtta agagagctgc agcctcagat tcttctatat ttataaatgg agaatcaggt
     9241 gtaggaaaag aaataatagc aagaacaata tataagtata gttcaaggag agacaaacct
     9301 tttatagcta taaactgtgg agctatacca aatgaattat tagagtcaga gttttttgga
     9361 tatgaagaag gttcttttac tggagctaag aaaaaaggta aaaagggact ttttgaagag
     9421 gcgaatggtg gaactatttt cttggatgag ataggtgagt taccaatgca gatgcaaaaa
     9481 aaattactta gggttataca agaaaatact attactagaa taggtggtag taagcctata
     9541 aaaatagatg ttagatatat aagtgctact aatatttctc atgaagacct tagaaataac
     9601 cttaaattta gacaagattt gtactataga ttaagtgtta ttccagtaaa aatacctcct
     9661 cttagagaaa gaaaagagga tatagtacca ttagtaaatt atttcttaaa actttacaat
     9721 gaaaagtaca atagagaagt agaagtttcg cctaaagtta ttgagttatt agaagaatat
     9781 tcttggccag gaaacataag agaattaaaa aacatcattg aaagatttgt agtattatca
     9841 gctaaaaatg taattggaga agatgaattt aacatgctga taaatttaga tatgattgat
     9901 aatgaaacag atgatttatc accaatagtg gtaaatggaa ttatgaatct taatgatgca
     9961 tataaaatag tggaccaaat aatgataagt aaggctataa ataagtatgg ttcaataaca
    10021 aaggctgctg aagttatagg tatatatcct tctaccatcc atagaaagat aaagagtgga
    10081 catattcacg tttagctgtc agtttaaaaa gacaatataa ttaaaatatt ataagataca
    10141 taatttatac aacttaaaag agatggctta aaaataaatt taattacttt aagtcatctc
    10201 ttttctttta taaatattta aaagttatat tagtttatag agttagcaga acctaaaaca
    10261 tcatctatct tgctttcaac tacagcttgt atagcatctc taccagcacc aatgtatttt
    10321 cttgggtcaa attcttttgg attttcagct aagaattttc tgatagcagc agtcatagct
    10381 agtcttaagt cagtatccat gtttatttta caaacagcca tagaagatgc ttttcttaac
    10441 atatctacag gtacaccttt agcaccagct atatttccac caaattcatt acatgtagca
    10501 acagcatttt ggtcaacagc agatgcacca tgaagaacta ttgggaaacc tggtaatttg
    10561 ctttggattt cttctagtat atcgaatctt aatttagctt ctcctttgaa tttgaaagca
    10621 ccatgagaag ttccaatagc tattgctaat gaatcaacac ctgttctttc aacgaactca
    10681 actgcctcag ctggttgagt atacttgtga acatcagaag taacatcatc ttctgttcca
    10741 gctagaacac caagttctgc ttcaacaaca acacctttag agtgtgcata ttcaacagct
    10801 tctttagtta ttctaacatt ttcttcaaag tcaaaatgag aaccatctat cataactgaa
    10861 gagaaaccag catcaataca agttttaata gcatccatat ttggaccatg gtctaagtga
    10921 agagctacat ctacacctat ttcatcagaa gcagccttaa ccatttcaac taatgtatga
    10981 ggtccagcat atttaacagc tgacatagaa gcttgtatca taacataaga atttttagct
    11041 ttagcagctt ttaaaacacc ttgtaattgt tctaagtcac ttatgttaaa tgcacctata
    11101 gcaaatccac cttcgtaagc ttttttaaac atttcttttg tagtaattaa tgccatttta
    11161 aactcctcct attaactttt aaaatttacc catcacttat attataacgt taaatcaaaa
    11221 aaagtacata gtaaattgta ataactataa attacaagta taatgttaaa tagaagacaa
    11281 taggattaaa taaaataata aagaatataa acaatttagg aaaattagct atataatgct
    11341 aactataagg gtaggaacta ccttattagt ttggagatac tgataacaat agttgtcttg
    11401 accaagaagt tgccacttca aacgttatcg aagggaatta agtggatgat aattcactaa
    11461 aaatttaaaa taaaggagat aaactatgcc acagataaaa ataagaggaa ttaatgaaaa
    11521 tgatatatgt aaaataagtg aaaaaatgat aaatgattta gttgaagctg taaaatgtcc
    11581 aagagactac tttgaaatag aatgtataaa gtcagttgca ataagagatg gtaaaatagc
    11641 agatgtatat ccttttgttg aagtagcttg gtttgataga ggacaagaag tacaagatat
    11701 agtagcaaga ataataactg atagtataag aaataattta gatgtagaaa gtatggattt
    11761 agcatttaca gtatttgaaa aagaaaaata ttatgagaat ggagagcatt tttaatggct
    11821 aaaaagcaac gtatagataa aattctttca aatttaggtt atggaagcag aagtgaaata
    11881 aagaagtatt gtaaacaagg gtctgtagta gtaaatggaa gtgaagtttc aaatccagga
    11941 actcaagtag acactgaaaa tgatgagata ttatttaatg gagaagaagt tatatataga
    12001 gaatatatat atttgatgat gaataaacct gctggatata tatcagcaac tacagataaa
    12061 tatgacccaa cagtattaga tttaattgat ttatcatact tagtttttga accatttcct
    12121 gttggaaggt tagataaaga cacagaggga ttattagttt tgactaatga tggaaagctt
    12181 tctcatagag ttttatctcc taagaagcat gtgcctaaga cttattatgc aaaaattgat
    12241 ggagtagtta ctgaagaaga tgtagaagct tttttagaag gtgtagtgct tgatgacgga
    12301 tataaaacta tgccatcaca gttaaatata ttaaaaagtg atgatgaatc agaaatagag
    12361 cttacaatac atgaaggtaa gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa
    12421 aaagtagtat acttaaaaag attgtctatg ggcaatttaa aattggatga aagtttagag
    12481 ttaggtgagt atagagagtt aacagatgaa gaagtaaaat tgattgaaga aagataataa
    12541 agatactata gtaaattgga ggaatgtctg tgaagaaaaa agatgttata gaatttgaag
    12601 tagataaaat ggaatttggt ggtacatctt taagccaagt tggagataga gttatccata
    12661 tgaagggtgg aataagtggt caaaaggtga gagctggagt taaaaaagtt agaagtaaga
    12721 aagctgaagt taagatgata gaattattag agaactcacc acttgaaact gaagaaactt
    12781 gcaagcactt tagagagtgt ggaggatgta cactattgtc tgtgccatat gaaaaacaat
    12841 tagaaataaa agaaaaacaa gttatggatt tatttttaaa gcaagattta tttggatttc
    12901 agtttttagg gatagaagga agtccagaaa acaaatatta tagaaataag atggaatata
    12961 cttttggaga tgaagtaaaa aatggacctt tgacattagg tcttcataaa aaaggaaagc
    13021 atatagatat acaaactgtt gaggagtgta tgttggttga tgaagacttc tcaaagatat
    13081 tagtttctag tgtagagttt ttcaatgaaa aaaagcttcc ttattataga acaatgaatc
    13141 acaaaggata tttgagacat cttgtagtta gaaaagggat tcacactaat gagattatgg
    13201 ttaatatagt aacttcttct caagaagatt ttgatatgta tgagtttaaa gatatgcttt
    13261 tagggattga tttaaaatct gagttggtgg gagtacttca tactattaat gatggtttag
    13321 cagatgcagt ccaatgtgat gagttaagag tattgtatgg aagagattat atacaagaag
    13381 aaattttagg tcttaagttt aagatttcac cattttcgtt tttccaaacc aatactaagg
    13441 gagctgaggt tctttatagt attgctaggg attttattgg agattacaat gataaagttg
    13501 tatttgactt gtatagtggt actgggtcta ttggacaggt tatggctggt gctgctaaga
    13561 aagtgtatgg tattgagata gttgaggaag ctgttgttgc tgctaatgag aacgctaagt
    13621 tgaatggact tactaattgt gagtttattg ctggggatgt ggctaaggtt gttaaggatt
    13681 taaaggacaa gcctgatttg attattgttg accctcctag accaggcatt cataaggatg
    13741 ctattaggga tatttgtgga tttggtgcta aggagattgt ttatatttct tgtaatccta
    13801 agtctttggt tgttgatttg gttgatttta aggggtatgg ttatgagatt aagatggtta
    13861 agtgtatgga tatgttccca aatacgccac attgtgagac tgtagttaaa cttattagag
    13921 aataacaggg tttcagactt ttatagtgtg tcttaaaagt agatttgcta ccataattat
    13981 ttttgggtgg caaatttatt ctacaatgat ttcaaaaata tttaccgtct ggtttctcat
    14041 gttattagtt acatgtaaat atgtatccat agttgttgaa gttttagcat gtccgagtat
    14101 atgctggata tcttttatat tagtaccatt ttctaataac atagtatcag ggtttttaaa
    14161 ataaagtgtg tattcttcta aagtagtgat aattaaaata tcaaatatca ctatacaaag
    14221 gaaagtgcac gctttttatg tttaacaaaa acgaaatatt aaaaaactta ttgaagaata
    14281 tgatgtcaaa acaactacag atattcaaga tatgctaaaa gatttattta caaatacaat
    14341 tcatcaaata tttacattgt cgaattttaa cggtttaaac ataatatatt gactgatata
    14401 ataaaatcat agaatatttt agggggaaat agattatgtt taagaaaaag ttaatagctg
    14461 ttgcaatgag tataactatg attagtgtag ggagcttttc gtatgctcat tcaggaagaa
    14521 ctgattcatc aggaggtcat cgtgataata aaaataaatc tggattagga agttatcact
    14581 atcattgtgg tggttatcca gctcatttac ataataatgg tggttgtcct tatactggag
    14641 gaggaagcag ttctggaaca acaacttcgg ttaataatga agaaaagcaa aagaggtctg
    14701 ttggagaaaa aggatataat caagggtatg aagatggata taaaggaaat tatagttcat
    14761 caaattatag tggagattat agtgatacat atgaatcaaa gtactcagaa ggatatgaaa
    14821 aaggtaaggc taaattagaa gaggaagaaa aggtggctaa agagactgga tataatttag
    14881 ggcttacagg tgctaaatct aataatacat atgaaaaaga agttttaaaa aatgcttatg
    14941 ataccggata ttcaaccgga tacaatgaat ataagactaa aaagatagaa gaatataaag
    15001 ctaaagggat agaggatagc aataaagata aagaaaaaat gacatttgaa gaaaacatag
    15061 attctgaatt tatagatgct tataataatg cttatgacga aatacaagaa caattaaaaa
    15121 atgattacac tactcaagga tttgagagtg ctatcaaagg agaaagattt gacacatcaa
    15181 ctataggtaa tgttaaatat gctaattggt ttaaagaagg atatgataat ggtaaggtta
    15241 agttacctaa agcaaaagaa agtgtatata atcaaggtta caatgaagaa gattttagta
    15301 taccagatga aatgaagtct attgaaacta agttaaaagg tgtatatgat gaaggtttag
    15361 aaaaaagaga ggaagagaaa agtagaaatg ctacttatgg ggttggagct ggaatagcag
    15421 ctgtagttgc tggagtagta tataagaaga aaaaacgtaa aaagatatag atgctagata
    15481 taccaatgat gacaagtgta taacaaatat aattttaagt ataaatattt aaaatataga
    15541 agggaaggtt atatctatgg gatttaactt taggaaaagc ataaatctag gaggaggact
    15601 caaattaaat ttaggtaaga agagtgtagg tataagtgct ggtgttaaag gtgctagagt
    15661 gtcagttaat tcaaaaggaa gaaaatcagc tacattgagt atacctggaa ctggaatatc
    15721 atatacaaaa actagtacaa agaaacgtaa acctaaataa atatttgata tactaagaaa
    15781 tagactgtta caaatagtct attttttagt atatagattt aaaaagtgac aaaattttga
    15841 aatataaact aatttcataa aggaaaatat tcttttatgt aaaattataa tatttaagta
    15901 atattataaa atttatagga ggatttcatt atgaaattaa gcaaaacact attggcaata
    15961 gggatgtctg tttcattatt gattgctagt tgtccaacat caaacgctct tagttctatt
    16021 aattcaataa aaggtacaaa taagtatgaa acagcaggtt taatagcaga taaacaaaat
    16081 tatgatattg caatacttat taattctgaa aatggattat ctgatggatt gtcagcaagt
    16141 ggattgtcag gagctaaaaa tgccccaata ttgttaacta agaaagatac aatcccttca
    16201 gaaacattaa aaagattaga aaaggtaaaa gaagtatatc taattggagg aaaaaattcg
    16261 ataggagcat cagttcagaa aatattaagt gataaaaaaa taagcataaa tagaataagt
    16321 ggaactgata gaatagatac aagtcataaa gttgcaaaag aaataggaag agtaagtacg
    16381 gttaaaaaag taatattaac taatgctttt aaaggcgaac cagatgctat gagtgttgct
    16441 tctatagcag ttagagataa atctccaata gtattaactg atggaaatac tacaagtttt
    16501 gctacagatg gattagaaac ttatgcaatt ggtggaaaag tcagtatgag tgataagtta
    16561 ataaatgaaa ctaaagcaac aagaataggt ggagtagata gatacgatac aaataaacaa
    16621 ataataaata aattttataa tggctcaaaa gaattttaca tagcaagtgg gactgattta
    16681 gtttattctt tagttggttc tacaattgca caaaaaacac ctatagtgtt agttggaaat
    16741 ggaagtaata agagtatatt aaaaaatgca agtaaaataa ctgctattgg aaatattgat
    16801 gatgttgtta taaaataatg tttaaatata actaataatg ttggggattc aaatacaggt
    16861 attgtaaaag atactaattc aaataatacg aacaaaccaa gtaatgagcc tagctctaat
    16921 aatcctggat taactgataa aagtacagta tatgtaactc ctaatggaaa atcatatcat
    16981 ctaacaaaaa attgttctac actaaaaaga agtaaaacaa taaataaagt aacattaaaa
    17041 caagctaagt cacaaggtaa aatagaccct tgtaatgttt gtgtaaaata atgtattagc
    17101 caaatatata ttaaaaaaga caattaatta agattgtctt
//
LOCUS       DAKBNN010000073        17116 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000073 DAKBNN010000000
VERSION     DAKBNN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.073"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            289..429
                     /locus_tag="OUB02_002006"
     CDS             289..429
                     /locus_tag="OUB02_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885763.1"
                     /translation="MEKSKKLLIDTGDTLNKLRENNSEKLEKYIELLKEYYKQENKKE
                     RE"
     gene            complement(444..809)
                     /locus_tag="OUB02_002007"
     CDS             complement(444..809)
                     /locus_tag="OUB02_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885764.1"
                     /translation="MIGYRIKELRKEKDITQKELATFLGLTPKMISFYEKEERFPPHD
                     IILKLSDFFNVSTDYLLGKVNVKNIDNLSELELIENLNFSDDIKEALKLISELSPSSQ
                     EKMFKIAKVFLEEELNEKK"
     gene            954..1190
                     /locus_tag="OUB02_002008"
     CDS             954..1190
                     /locus_tag="OUB02_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885765.1"
                     /translation="MNKLKDFRIQKGLSRNQLGAELGLTSRYIAFLENGERVPSLNTA
                     VKIAAYFNTSIEYIFLKNNCTKSTFKEKGAEKYE"
     gene            1183..1974
                     /locus_tag="OUB02_002009"
     CDS             1183..1974
                     /locus_tag="OUB02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCT8885766.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            2044..2373
                     /locus_tag="OUB02_002010"
     CDS             2044..2373
                     /locus_tag="OUB02_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885767.1"
                     /translation="MSVALQFIDTKDLVQELMRRDDTTDIIKMFLGREGIKRMELMTI
                     EEFCEYLKISDVTARNMAREAMITKDFIALKIGRKYMIDRISFEEFIMKNAMKDRDVM
                     KKRKGVI"
     gene            2679..2924
                     /locus_tag="OUB02_002011"
     CDS             2679..2924
                     /locus_tag="OUB02_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885768.1"
                     /translation="MKSRQELIKDIEKYRKAQYLIYLDIVQRAWADRSLTADEQDRIK
                     QEAYAEYKRIERDTEEAEELLMREEFETDRPLAVQIM"
     gene            3007..3516
                     /locus_tag="OUB02_002012"
     CDS             3007..3516
                     /locus_tag="OUB02_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCT8885769.1"
                     /translation="MSTLYELATDLLEIEEGLTEITGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            3526..4110
                     /locus_tag="OUB02_002013"
     CDS             3526..4110
                     /locus_tag="OUB02_002013"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HCT8885770.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIIFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDATSGKNEAKSNSNNRILTDKQLSRLYAIASNANVDK
                     ESLKEKVFKRFGKEIKDLTKQEYDTICNAYENKQ"
     gene            4125..5006
                     /locus_tag="OUB02_002014"
     CDS             4125..5006
                     /locus_tag="OUB02_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCT8885771.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKSKQEKKQSNNKAVNTHNVNKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            5065..5238
                     /locus_tag="OUB02_002015"
     CDS             5065..5238
                     /locus_tag="OUB02_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCT8885772.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            5254..5661
                     /locus_tag="OUB02_002016"
     CDS             5254..5661
                     /locus_tag="OUB02_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT8885773.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKAKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            5730..5951
                     /locus_tag="OUB02_002017"
     CDS             5730..5951
                     /locus_tag="OUB02_002017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885774.1"
                     /translation="MRFEIGKTYKFDREKFIACNGIEHYKRCEELWIEDVEDVEFTVK
                     KIFDDGYICYPKEFWFNFGVVSEWCVEVK"
     gene            5969..6349
                     /locus_tag="OUB02_002018"
     CDS             5969..6349
                     /locus_tag="OUB02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885775.1"
                     /translation="MAKIWVDAGTFLEKTIDIEDMFELNLRKVRKRNEKIKNEIEKKI
                     KDEDSEIKITNRGGPGKSIKVFNINTREVKILKSAKEASKYIKVSCSHASYLARENKS
                     TEDGWKVEYIQEVSDGISKCGTSN"
     gene            6318..6467
                     /locus_tag="OUB02_002019"
     CDS             6318..6467
                     /locus_tag="OUB02_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885776.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGML
                     RNESN"
     gene            6454..6903
                     /locus_tag="OUB02_002020"
     CDS             6454..6903
                     /locus_tag="OUB02_002020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885777.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWQKVC
                     NVGICSEEWAANNQEIFEGDILKENYNPRNRSSYGIVKRDFNSIKLYLEWHYLKKLEG
                     EWIELINKTEIYHSRDYKIVGNEFDNLEEVRAEFLERKESLENEYLS"
     gene            7005..7181
                     /locus_tag="OUB02_002021"
     CDS             7005..7181
                     /locus_tag="OUB02_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885778.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            7295..7984
                     /locus_tag="OUB02_002022"
     CDS             7295..7984
                     /locus_tag="OUB02_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885779.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
     gene            8002..8223
                     /locus_tag="OUB02_002023"
     CDS             8002..8223
                     /locus_tag="OUB02_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885780.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEV"
     gene            8220..9215
                     /locus_tag="OUB02_002024"
     CDS             8220..9215
                     /locus_tag="OUB02_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCT8885781.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLS
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKAIKIGALIG
                     LSGINDEYHLKVLDKDAEKEEANSKFVTEFLNATKVKDDKYKTKMFKAFVDSYIAHLY
                     SDMKQGEDVRGMLLYMLREKQKLDINEFADKAIKDDLKDSFKDHIEEKGIESFNIDKK
                     WVEKNLKNRCIKTDTGFEIKGKMEYFEDFMKYGIRHNGNGTVDIVIKNVNFYNEK"
     gene            9224..9949
                     /locus_tag="OUB02_002025"
     CDS             9224..9949
                     /locus_tag="OUB02_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HCT8885782.1"
                     /translation="MKYVASFSGGKDSAAMLLLILEKGLKLDEIVFIDTGLEFKEIYD
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNQQGKRKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNKLGEHKRYIGIAFDEPSRYKRLEKNCIAPLFEEKMTEKDCLKYLKEKGFYYE
                     IHHRFKRTGCYLCPKQSLESLRTLRRYYPDLWGDMLKLDKDSPVPFRANGVTVNDLEK
                     RFSNEDIENERQISFFQTEKVSI"
     gene            9946..10113
                     /locus_tag="OUB02_002026"
     CDS             9946..10113
                     /locus_tag="OUB02_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885783.1"
                     /translation="MTNKEMCKSKNLDEREVCKKFRKEICASCINDKGDCESKDCDTA
                     YKNWLEDIYYV"
     gene            10106..10480
                     /locus_tag="OUB02_002027"
     CDS             10106..10480
                     /locus_tag="OUB02_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885784.1"
                     /translation="MYSTYKVKLKTKKTLEQLRKQGDINFGSSEEGLKRALKYNNLID
                     GLEVTVIKFKDVYCLANCDEKDVEKIRDAYYLLEQDKYFGCYINEYERFKKDWKNGSC
                     DGEASMVFSDDEIEIIEKLREG"
     gene            10483..10665
                     /locus_tag="OUB02_002028"
     CDS             10483..10665
                     /locus_tag="OUB02_002028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885785.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            10763..10936
                     /locus_tag="OUB02_002029"
     CDS             10763..10936
                     /locus_tag="OUB02_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885786.1"
                     /translation="MNKCEGCKYRAYEEDGEVYFYSDGSYEGGICREEYCSRGYEKKF
                     ENNGENCKSFKKL"
     gene            11035..11250
                     /locus_tag="OUB02_002030"
     CDS             11035..11250
                     /locus_tag="OUB02_002030"
                     /inference="COORDINATES: protein motif:HMM:NF024363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HCT8885787.1"
                     /translation="MIHELQIASSTFKDVIKNKKQIEVEYTTDFKVGDTVILQSFKDK
                     KFTGIEINKEIEYFVKLGNGYVELGIK"
     gene            11268..11711
                     /locus_tag="OUB02_002031"
     CDS             11268..11711
                     /locus_tag="OUB02_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCT8885788.1"
                     /translation="MKVNFVIDGEPKAKARPRMSTSTGKAYTPDQTILYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALQKNKVNTKAYKDAIGKVEGLIRPNKKPDL
                     DNIIKSVADSLNGIAYKDDSQIVEVVSKKYYSDKPRVEVVLEDII"
     gene            12025..12879
                     /locus_tag="OUB02_002032"
     CDS             12025..12879
                     /locus_tag="OUB02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCT8885789.1"
                     /translation="MNENINKEITVLGTLEVEGMKFHDIEGGFGKGKKAMLVKDIAEI
                     HNRELKHVNELINSNIKRFKTNVDIIDLKVSRSERLGDLKANPSKGLGYEDVGYSKQS
                     FNQSRNIYLLSERGYSKLLKILEDDKAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSKA
                     YKNKSLRGKVYSDIYYQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEDEIRLL
                     NSQLSIVS"
     gene            12979..13485
                     /locus_tag="OUB02_002033"
     CDS             12979..13485
                     /locus_tag="OUB02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885790.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSTNIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNVIEDAVVNISDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIVHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            14239..14412
                     /locus_tag="OUB02_002034"
     CDS             14239..14412
                     /locus_tag="OUB02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885791.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            14412..15023
                     /locus_tag="OUB02_002035"
     CDS             14412..15023
                     /locus_tag="OUB02_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCT8885792.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGGYLVQMLKYLKK"
     gene            15099..15356
                     /locus_tag="OUB02_002036"
     CDS             15099..15356
                     /locus_tag="OUB02_002036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885793.1"
                     /translation="MNDYKEKTEPKLNILVQSKVDILCSNSSIMNGVDTSIDIKLSLD
                     NRVICNLTNEDIKKEISTSFNKCLKEIDKKICSKEVINKLI"
     gene            complement(15340..15858)
                     /locus_tag="OUB02_002037"
     CDS             complement(15340..15858)
                     /locus_tag="OUB02_002037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885794.1"
                     /translation="MNFEDKLKNITAEAHAEEQLEKEKSIQRAMNLEAQRNITLELKS
                     IIEAQFETIMKYYPNASYEKTHTIKDGEIVAIMGVFECCFLEFTKNDTYSIKNPSIIC
                     FRIFPEDDENFKLISLTLTNTENSSNPYYEFLRKSKRINPFYSGSLDKELIIEKVQEQ
                     LISSIEEIKSAY"
     gene            15984..16469
                     /locus_tag="OUB02_002038"
     CDS             15984..16469
                     /locus_tag="OUB02_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCT8885795.1"
                     /translation="MEIVKNESEKFEELKKKAKDKTNWRNRLEAVIELKDYDCKESID
                     IVTNLALHDRVFKVKEEAFRVAQSLNVTKRGNPIHLGRKDIGYKNKDFVKVFEKIKRE
                     CNMDEFDLDIFKEKFKVVNPEMYDVMKFEKCDQFEECDIDKSEQFDKWIENMFKSLPK
                     K"
     gene            16530..>17116
                     /locus_tag="OUB02_002039"
     CDS             16530..>17116
                     /locus_tag="OUB02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885796.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEIASKYDISLNTLKSWIKRYNWSKE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETQDLINEIKNKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMVKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKT"
BASE COUNT         7225 a         1639 c         3187 g         5065 t
ORIGIN      
        1 acaagaaaat acaaaaaatt ataattaaaa agacgattaa taatacacag gaatggaaaa
       61 aataaccaag actctacaaa attcgatttt tttgttacaa ttcccctttt tttattgcat
      121 atatttaaca aaagtattat tatgtaagta agataattat ccaagaaaat tttgaataat
      181 ctaaaaatat ttttagaaat gaaaatacat agttatattt tattaaaatt cattagggaa
      241 ttttgactaa aaataagaac gtaagttctg atagtagggg gaaggcgtgt ggaaaaatca
      301 aaaaaactac taattgacac aggggataca ttgaacaaat taagagaaaa taatagtgag
      361 aagttagaaa agtacataga attattaaaa gaatattata aacaagaaaa taaaaaagaa
      421 agagaatagt tttcttctct ttcttatttt ttctcattaa gttcttcttc aagaaatact
      481 tttgctattt tgaacatttt ttcttgacta gaaggactta attcgctaat tagttttaaa
      541 gcttctttta tatcatcaga gaaatttaga ttttcaatta gctctaactc actaagattg
      601 tctatatttt ttacattgac ctttccaagc aaataatctg tagatacatt aaaaaaatct
      661 gataatttta aaattatgtc atgtggagga aatctttctt ccttctcata aaaagaaatc
      721 attttaggag taagacctaa aaaagttgca agttcttttt gagtaatatc tttttctttt
      781 cttaattcct ttattctata tccaatcatg ttatttagcc acctttttaa tttatattga
      841 actattagtt cttaattatc aattatagct catttttttt aaaaaagcta ttgacaatgt
      901 actaatagta ctgtactatt agtacataag ataaagtaaa agaaggtgaa aaaatgaata
      961 aactaaaaga ttttagaata caaaaaggac taagtagaaa tcagttagga gctgaattag
     1021 gtcttacaag taggtatata gctttcttag agaatggcga aagagtaccg tctctaaata
     1081 cagctgtaaa aatagcagct tatttcaata cttcaataga gtatattttt ttgaaaaaca
     1141 actgtactaa aagtacattt aaagaaaaag gagctgaaaa atatgaataa cttacaagta
     1201 atagaaagaa ataacgaaag agttttaact acacagcaac tagcagatgt atatgaaaca
     1261 gatgcaagaa atataagcaa caattttaac aataacaaag atagatttat tgaaggtaaa
     1321 cattattttt tattgcaagg tgatgattta aaaaatttta aaggtattca tacagaatat
     1381 gaaaacctaa aatttacttc aaaaatgtat ctttggactg aaagaggagc aaatagacac
     1441 tgcaaaatat tggatactga caaagcttgg gagcagtttg ataacttaga agaaacatat
     1501 ttcaaagtta agcaacataa gccaacttgt atagaagatg tattaataga aagtttaaaa
     1561 gaaatgaaag atttaagact tcaagttaat caagcaaata gcattgcttt agaagcaaag
     1621 acagaggttg aaacaataaa agatgtagtt tcattagact caaatagttg gagaacaaat
     1681 acacatcaac taattgcaag aatagcaaaa aaacaaggtg gttttgaaca tataaatatg
     1741 cttagaacag aaagttatga attattaaat aagagatttg gagttgacct acatagaaga
     1801 ttaatcaata aaagaagaaa aatggcagaa gaaggtgtat ctgaatctaa aagagagaaa
     1861 gttaacaatt tagatgtaat acaagatgat aagaagctaa tagaggggta tgtggctatt
     1921 gtaaaagata tggctttaaa atatggaata tcaagtgatt taagcaaaaa ttaggttaaa
     1981 tcatgacagt accttgaaaa ctaaatacag gatattcaat taatatgaga aggaggtttg
     2041 aaaatgagtg tagcattaca attcatagac acaaaagact tagtacaaga gttaatgcga
     2101 agagatgata caacagacat catcaagatg tttttaggta gagaaggaat taaaagaatg
     2161 gagttaatga ctatagaaga attttgtgag tacttgaaaa tatctgatgt aacagctaga
     2221 aacatggcaa gagaagccat gataacaaaa gattttattg ctttaaaaat aggaagaaag
     2281 tacatgattg atagaatatc atttgaagaa tttattatga aaaatgcaat gaaagataga
     2341 gatgtaatga aaaaaagaaa gggggtgatt taattgactc taagatggtt tttaagattt
     2401 tgtaaaaaac ataagaaagt gcctacacca cgattttatg cagagtgtgt agcttatatg
     2461 gaagaatgta aacaaagagg acttgaactt taagggggat tgaatatgga agtaactaaa
     2521 agatttttag aatataaaat acaggctcta agcgagagga tagaatttaa aaaaaacaat
     2581 tggatataag tgtatagcag atgaaaaaga actaggagca tatgaggata ttttgcttat
     2641 gttaaattca gaaattgaat caataggagg agtggaaaat gaagagtaga caagaattaa
     2701 tcaaagatat agaaaaatac agaaaagcac aatacttaat atatttagat atagtacaaa
     2761 gagcatgggc agatagaagc cttacagcag atgaacaaga cagaattaaa caagaagcat
     2821 atgcagagta caaaaggata gaaagagata cagaagaagc agaagaactg ctaatgagag
     2881 aagaatttga aacagataga cccttagcag ttcaaataat gtagaaaaga gccactgcaa
     2941 tggctctaat caaaaacaaa aaataaattt aataattata tattatagca taaatggagg
     3001 gaaattatga gtactttata tgaattagct acagatttat tagaaataga agaaggttta
     3061 acagaaataa caggaaatga aactgaaaaa ctagaggaaa taaaagaaat aataaaacaa
     3121 gagatacaaa ataaaaacac taggatagtt tcagtaatat taaacattga cagtgatata
     3181 aactctatag attcagagat taaaagattg caagagttaa aaagggtcaa aaagaatact
     3241 cttgatagat taaaaagcaa tataaaagac tgtatggaat tacttggtac taaaaaagta
     3301 gaaacagttt taggaaatat aagtataaga aagtcagcag gtagcttagt catagaagat
     3361 gaagaaaaga tacctgctat atataaaaca gtagagcaag ttgtaaaagt agataagaac
     3421 actattaaag attttattaa aaaaggtcat gaggttgaag gttgcaggat tgaatatgga
     3481 actacactaa caattccaaa agctaaaaaa gagtaggtga ggaccatgga aattaataat
     3541 atttacatta aattgatgga tgtaagagtt aaatttagta agttgaatct aaagaaaagt
     3601 ggagaaaata agttcgctaa cttcaagtat tttgagttag cagactttct accacaagca
     3661 actggattac ttgaagaagc taagctatgc cctatagtga cctttacaaa tgaatatgca
     3721 actctaacat taattaatgg ggaaaaccca tcagagcaga ttatattcac ttctccaatg
     3781 cgagacttac aacttaaagg ctctaatgaa ttacaagcac taggaggtat agaaacctat
     3841 caaactagat atttgtacat tcagttactc aacataactg aaagtgacac ttttgacgca
     3901 actagtggca aaaatgaagc taaaagtaat tctaacaata gaattttaac agataaacaa
     3961 ttaagtaggt tatatgcaat agcaagtaat gcaaatgttg ataaagaaag tttgaaagaa
     4021 aaagtattta aaagatttgg aaaagagata aaagatttaa caaaacaaga gtatgacact
     4081 atttgtaatg cttatgaaaa taagcaataa gggcaggtga tattgtgggg attataagag
     4141 taagcaaaga caaagataat ccatatgtag ttttaaataa aacttgtttg gaagatgtga
     4201 aattaagctg gcaagcaaaa ggtttacatt catatctgat tagtaagccc gaccactgga
     4261 aaatctatgt taatgattta tataaaagaa gtaaaaatgg gagggatgct acagcaaata
     4321 ttttaaggga gctcatagaa aatggatata taacaagaac accttgtcga gattctaata
     4381 ctaataagat gcttggagga tatgattatc aagtatatga gataccactt gaaaatcctc
     4441 agaagctaaa atcccgaaaa actgatttcc cggaaaccgg atttcccgga aaccgggttt
     4501 ctcggaaacc ggaaaacacg gaagtagtaa gtaatgactt taaagtaaat aatgatatta
     4561 ctactattgt tattaatgaa caatccaata aagacaaaac cacctacata aaaaaatact
     4621 ttgaaaaata tataggtgtg attactccta ataactttat agagttaatg agttacttag
     4681 atgatggaat ggaagctgat gtaattataa gagctattga tgaagcaata gcaaatggag
     4741 ttaagaatta taagtatgta aagacaatat taaataattg gatagaagca ggtgtaaaaa
     4801 ctaatttaga acttacagag tatcaaaatg agtttgagag gaagaaaaag agtaaacagg
     4861 agaagaaaca gtctaataat aaagctgtga atactcataa tgtgaataaa aataagtttg
     4921 ctaactttaa tcagactttc actcaatatg aagaaaaaga gctagatgag attattaaaa
     4981 agagtcagaa ggaaaaattt aaataaaatt aaacttctag gaagtaaata tcaatatatt
     5041 gcttcctgga aaggggaggt ataaatggcg agaatatatg cacaaagaag tggttcttta
     5101 aatgaacaag atagattgga attattaaga ttacttggga aagctggata tacagtaaag
     5161 attgctagag agaagcaaaa tagcaagaca acttatactt actttgttga gtatacagaa
     5221 gagcaggaag aaaaatagaa gggggctagt taaatgaata caataacttt agttggaaga
     5281 ttagttgcag atgcagaatt gaagtacctt ccaaattcgg gtactccaaa aataaccttt
     5341 tcaatggcag tagatagaag gtttaaagat aaaaatggaa ataaaataac tgattttatt
     5401 caatgtgagc aattaggaaa acatgtagag aatctagtgc aatatcttgt taaagctaag
     5461 cctatatatg ctgttggaga gttaaatata tataattaca aagatgaaaa tggttgctgg
     5521 aaatctatta ctaaggttaa tgtaaatgct ttagaactac tttctagtaa aaatgataat
     5581 aatgctaaac aagaatatgt accaccagga ctggacccac aaggttttca agcaatagat
     5641 gatgacgata taccttttta attaagttaa atagtctagg gagtaactat acaatattac
     5701 ttcctaggag ttaaaaatgg agggataaaa tgagatttga gataggtaaa acttataagt
     5761 ttgatagaga gaaatttata gcatgcaatg gtatagaaca ctataaaaga tgtgaagaac
     5821 tttggattga agatgttgaa gatgttgaat ttactgttaa gaaaatcttc gatgatggat
     5881 atatttgtta tccaaaagaa ttttggttta attttggtgt agtttcggaa tggtgtgttg
     5941 aagttaaata aggtaggagg aattagatat ggctaaaatt tgggtagatg caggaacgtt
     6001 tttagaaaaa actattgata tagaagatat gtttgagctt aatttaagaa aagtaagaaa
     6061 gagaaatgaa aaaataaaaa atgaaattga gaaaaaaata aaagatgaag attcagaaat
     6121 aaaaattaca aatcgtggtg gaccaggaaa gtctataaaa gttttcaata taaataccag
     6181 agaggtcaag attcttaaga gtgctaaaga agcaagtaaa tatataaaag ttagttgcag
     6241 tcatgccagc tatttagcta gggaaaataa atcaactgag gatggttgga aagtagaata
     6301 tattcaagag gtgtcagatg gtattagcaa atgtggaaca agtaattaag ttagctgaaa
     6361 agatattaaa taagaaaaag tgttctgtta ataaagctat tgatatagct ataaaaatat
     6421 taagtaaata tgagtgcgag gggatgttaa gaaatgagtc taattgaata tagaggttat
     6481 gattttgaaa ataaaaagtg gatttattct aaaacaataa tgtggagtaa tgctgtagac
     6541 tgtttattaa tgttgaaaga agattttaaa tggcaaaaag tttgtaatgt tggaatatgt
     6601 tctgaagaat gggctgcaaa taatcaagaa atttttgaag gggatatatt aaaagaaaat
     6661 tataatcctc gcaatagaag ttcatatgga attgtaaaaa gagattttaa tagtattaag
     6721 ttatatttgg aatggcatta tttaaagaag cttgaaggag aatggataga gcttataaat
     6781 aaaacagaaa tatatcatag tagagattac aaaatagttg gtaatgaatt tgataattta
     6841 gaggaagtca gagcagagtt cttagaacgt aaggagagtc ttgaaaatga atatcttagc
     6901 tagtgcgata ttagtaatag gaagttttat agctggtagg gtttatgagt atagattgaa
     6961 tctgaaagag tgtgaaaatt gcgataatat gagagggctt aagaatgaat gatagattag
     7021 aaatgataaa tgcttctgta aattatatac agatgatatg tgaaagttca aatatagcta
     7081 ttatagcaga gcgaggaaga gttagaatat tagatttaga aactaaagaa aaatatgatt
     7141 tattaaaaaa taaactcgag gaaatgttag aagaaatata agtgaaaata tctaattaaa
     7201 acagtttaga gagttgcaaa atgtcttttg gtataaatta ttgttgaagt gttttgtgac
     7261 tctcaaaaat gaaaatagag aggggaataa tataatggat ttaaataaaa ttatgaatga
     7321 tgcactagta gagttagagg aaaatggttt tgtagaggaa gtagttaaaa aacaattggc
     7381 agaaacaata aaaagagttg tgaatgaggt ttttggaaat tggggtgatt ttagtaaaaa
     7441 aattgaaaaa catctaagcg aaaatataaa tataaattta gataaattgg atatacaaaa
     7501 atataatgtg ctcgtagcaa ctacagtaaa agaaaagatt gatatgacaa tgaaggtaga
     7561 agctattgaa cacttaaaaa gaaatttaga taacatgctt gtaggtgctg aaaaagaata
     7621 taaaatgagc gaattgttgg aaatattaaa aatggataaa tatgatattg atgaatatga
     7681 aggctatgaa gattgcataa catttattct tgatgaaaat aaatatggtt cgggatggat
     7741 tgaaattgat aaagaaccaa ataaaagtaa atacagatgc gaacactcta tattgttgag
     7801 aaaagatgga actattgcat catatagata cgagaataga gaaatatcat ctaaagatat
     7861 aatgagtgga tttgataatt ttggagattt gctatttaaa atatttgctc atgggtcaaa
     7921 aataattttg gatttaggaa atgatataga agattatgat ttaacaaatg gagaagatta
     7981 ttaaaatagg ggatggaaca aatgtcaaag tatgtattga gatggcaaaa aggattatta
     8041 ctagatgaac gcaagataaa ttattcatgt ggaagtaaag aaatgttaaa aaagaaagct
     8101 gaattattag ctaaagatga caagatactg cttgtaacaa tagataaagt tgaagaagtt
     8161 ataaaagata ctagaagcca aaaaatggct gaatattttg aggatgaagg ggtggaagta
     8221 tgataataca caaatttata atacatgttt tagataagaa cagcgataca ccaatactaa
     8281 atgattttga aggtagagtt agtcaagata ttgaagcttt ctttcaaaag aaaataagca
     8341 aagtatcaag agataatgat atcagaacag cagtatttaa tgactatagt aacaatctaa
     8401 ttaagaagtg ttgtgaacaa attatttatg atgaaagttc atttttaaat aactctaaag
     8461 agattgcagc ttatttattt gatgttatga agcttaatgc tacattagaa tcttgcgact
     8521 tatcaatttg cttatactct caaaaagatg aaaagaaagt tgctatatta aagcttgatt
     8581 acaataattc gtatactcat tcaattgagt ttaaagatga taaatttaat atacagatgt
     8641 ctaaaaatga aattaatata caagagacta aagcaattaa aattggtgca ttgattggat
     8701 taagtggaat taatgacgaa tatcatctaa aagtattgga taaagatgca gagaaggaag
     8761 aagctaattc taagtttgtt acagagtttc taaatgctac taaagtgaaa gatgataagt
     8821 ataagactaa gatgtttaaa gcttttgtgg actcttatat agcacattta tatagtgata
     8881 tgaagcaggg cgaagatgta agaggaatgt tactttatat gttaagagaa aaacaaaaac
     8941 ttgatataaa tgagtttgct gataaggcga taaaggatga tttaaaagat agttttaagg
     9001 accatataga agaaaaagga attgaaagtt ttaatattga taaaaagtgg gttgaaaaga
     9061 atctgaaaaa cagatgtatc aagacagata caggatttga gataaaaggt aagatggagt
     9121 actttgagga ttttatgaaa tatggtatta gacataatgg gaatggaact gtagatatag
     9181 ttattaagaa tgttaatttc tataatgaga agtaggtgct catatgaaat atgtagctag
     9241 ttttagtgga ggaaaagatt cagcagcaat gcttctttta atattagaaa aaggtcttaa
     9301 attagacgaa atagttttta tagatacagg attagaattt aaagaaattt atgatataat
     9361 agatgatttt gaaaaaagaa taaactttaa aataacaaga attaaagcag aaaaaacctt
     9421 cgaggaatac ttttatactg ttaatcaaca aggtaagcgt aaagggcaaa tatggggttt
     9481 tccgtatact ctaggggcat ggtgtaatag cagattaaaa cttgctcctg ctaataaata
     9541 ttttaataaa ctaggagagc ataaaaggta tattggaatt gcctttgatg aacctagcag
     9601 atataaaaga ctagaaaaaa actgcatagc accgttgttt gaagaaaaaa tgactgaaaa
     9661 agattgcttg aaatacttga aagaaaaagg tttctattat gaaatacatc acagattcaa
     9721 gcgaactggt tgttacttat gccctaaaca aagcctagag agtctcagaa cattgagaag
     9781 atactatcct gatttgtggg gggatatgct taaattggat aaagatagtc ctgtaccttt
     9841 tagagctaat ggggtcacag taaatgacct agaaaagcga tttagtaatg aagatataga
     9901 aaatgaaaga caaataagtt tctttcaaac agagaaggtg agcatatgac taataaagaa
     9961 atgtgcaagt caaagaatct tgatgaaaga gaagtgtgta agaaatttag aaaagagatt
    10021 tgtgctagtt gtataaatga taaaggagat tgcgagagta aagattgtga tacagcatat
    10081 aaaaattggt tggaggatat ttactatgta tagtacttat aaagtaaaat taaagactaa
    10141 aaaaacatta gaacaattaa gaaagcaagg agatataaat tttgggagtt cagaagaagg
    10201 attaaaaaga gcattaaaat ataataattt aattgatggt ttagaagtaa ctgtaattaa
    10261 gtttaaagat gtatattgtt tagctaactg tgatgaaaaa gacgtagaaa aaataagaga
    10321 tgcatattat cttttagagc aagataaata ttttggatgt tatataaatg aatatgaacg
    10381 atttaaaaaa gactggaaaa atggtagttg tgatggggaa gctagtatgg tattttcaga
    10441 tgatgaaatt gagataattg agaagctaag ggagggttaa atatgaataa aagaattaaa
    10501 atgaaaaaaa gattaattca taaaaagtgt gatgaaagat gtgtcaacta tgactttgta
    10561 attagcaata accttataac ttgtaatgtg tgtataggat gcaaatacaa agaaaatatg
    10621 gataaagtat gtgaagagaa ctataagaaa ttaagaagta aatagaataa aatagtcaag
    10681 gtaagtttgt gaatgaaact aaaagtctat agacttactt tgacttataa aagggagttt
    10741 ttaaaaaata ggaggtaata aaatgaataa atgtgaaggt tgcaaatata gagcttatga
    10801 ggaagatgga gaagtttatt tttactcaga tggaagttat gaaggaggta tatgtagaga
    10861 agagtattgt agcagaggat atgaaaaaaa gtttgaaaat aatggagaaa actgtaaaag
    10921 ttttaaaaaa ttgtaggacc ataaaaaata aaatagagtc attggtattg ctatatttta
    10981 ttaaaaaata taaaaaagtt tttgagaaaa ttaactttta aagggtgtga gcttatgata
    11041 catgaattgc agatagcttc tagtactttt aaagatgtaa tcaagaataa aaaacaaatt
    11101 gaagtagagt atacaacaga ttttaaagta ggagatacag tcatattgca gtcatttaag
    11161 gataaaaagt ttacagggat tgagataaac aaagagattg agtattttgt taagcttgga
    11221 aatgggtatg ttgagttggg aataaaataa aaatttaagg agagataatg aaagttaatt
    11281 ttgtaataga tggagaacca aaagcaaaag cacgtcctag aatgagtaca tcaactggca
    11341 aggcttatac acctgaccaa accatactct atgaaaattg gattagactt atgtacaatt
    11401 ctacagtaaa gcatttcttt gagggtaatg tgaaaatgac agttatttgt tactttgaca
    11461 ttactaaaaa agatagggaa gcactgcaga aaaataaggt aaatactaaa gcgtataagg
    11521 atgctatagg gaaggtagaa gggttaataa gaccaaacaa gaaacccgat ttagacaata
    11581 taatcaagtc tgtagctgac agtttgaatg gtatagctta taaagatgat tctcaaattg
    11641 tagaagtggt gagtaagaaa tattatagtg ataagccaag agttgaagtt gtactagagg
    11701 atattatata aaaagaatct aaataaaagt cacaagaaaa ttattaatat actctataaa
    11761 aaacgatatt aataaaatta aacaaatcca tataaagata tgttttttaa atggatatgt
    11821 cttattgagt ttttgtgcag cagatgccag taaaaaataa atacaaagtc ttattaagaa
    11881 aactattaaa tctcccataa aatcctccta agattagatg atataaggtt aggatactat
    11941 gagatattaa taataacaat agattttgca gaactgtaaa taaattgaaa tgaattattc
    12001 aaaagagaaa aggagagata gattatgaat gaaaatataa ataaagaaat aacagtactt
    12061 ggaactttag aagttgaggg aatgaaattt catgatattg agggtgggtt tggaaaaggc
    12121 aagaaagcaa tgttagtaaa ggatatagct gagatacata atagagaact taaacatgta
    12181 aatgagctaa ttaatagtaa tataaagaga tttaaaacaa atgtagatat tatagatttg
    12241 aaagtgagtc gttctgagcg actaggtgat ttaaaggcta atccttcaaa aggattaggt
    12301 tatgaagacg ttggatatag caaacaatct tttaatcaat caagaaatat ttacttgttg
    12361 tctgagagag gttattcaaa actacttaaa atactggaag acgataaggc ttgggagcaa
    12421 tacgagaaaa tagttgatgg atatttctct atgagaaaag aactaaataa tcctctttta
    12481 agtgcatcaa aggagttaca ggctatattt atgctagata agaaacaaga agtcttagaa
    12541 actaaaatag agaatgttaa tgagaagtta gagaacttta tggatgatgc accactattc
    12601 aatattgaat gtgaaagtat tgtaaaagaa gttaagaagg tagcaacaaa atcactagga
    12661 ggtcatggaa gtaaggctta taaaaataaa tctttaagag gtaaagtata cagtgatata
    12721 tactatcaga ttaaacgaga gtttggagta gatagttata aggctataaa gcgttgtcag
    12781 ctagataaag tattagagat tgtaaataat tataaattac ctatagtgtt tgaggatgaa
    12841 ataagacttt tgaatagtca actatcaata gtaagttaaa agaaaaaagg agtgctttca
    12901 cactccactt gtcaaaaata taaaactttt atatacaaat attattataa cataaataat
    12961 tgataggagt gtgaatgtat gtctaaaact aaaaaagagt tttttaatgc aactaagaaa
    13021 caactttcta attataagca attaagtaca aatataataa aattgaaaaa tgaaatacaa
    13081 atgttgaaag ataattcggt tggagattta atgaaaggta taagttatga tagtgtcaaa
    13141 acaggtaaaa caaacaaaac tagtaacgtg attgaggatg ctgttgttaa tatatctgac
    13201 ttaataacag aaaaagaaat agagctatat gaagcagaaa taattaaatc tacaatagat
    13261 ttagctataa ggaatttaaa acctatacac agacaaatta ttgaacttaa atatatagat
    13321 ggtctaatgt ggcaagaaat ggttgatata gtacatttag aagaaagaca attaagtgta
    13381 agagctagtc aagctattag ctcaatatca atagcactgt ttgggaagaa agcattaata
    13441 gagcaagaac cactttttga attgttagat tacaaactaa attaaaaagt aagaataatt
    13501 ttgagtgctg aaaatgtgca ggtttttttg ttttagacat gagataatag tattgtggaa
    13561 ataaagattt ccctctcaaa acttaatatt tgactagggt taagggattg ccctagtcac
    13621 tatgaacaga ctaggcaggg cgtgaggacg ctgttagttc aattctaact atgttcaatc
    13681 tttagttttt ttctatttca attaatctac agatacacta aaaaaatatt agatgaattg
    13741 agattaaaat ctcatacaat tttgtatctt aattcagagt ctaaaaccga gtggggcttg
    13801 gtaacctcac tcaccatgca ggtactggtg tttaatctag gttcgattcc tagaacttgc
    13861 gacataatat atgtatctcc ctactaaaaa tgctaagttt actccaaact tagcatttta
    13921 ctttttaaaa agaaaaaaga aatttttatt gtcataatac tatttgttta ggtatattat
    13981 aatgtgcata cttaaaatta aatacttagc aagcatttga attaatatac ataagatata
    14041 tgacataatt ttttatagtg taagttattt aaattaatta taaataacag taattttatt
    14101 atataaaatg tacatattgt gaataataat aataaaatca tgtacaaaat gccaactgat
    14161 aattcctcga aatgtattgc atatttaacg taacgtcagt ataattaaat tataatagtg
    14221 aagtggaagg tggtacttat ggctacaaaa agtattttaa aaaatgtaga tgtaagaaaa
    14281 aaggcatttg gaagaaatct agtatcagct ctagaaaatg ctaaaaataa acaagaaaaa
    14341 gaagttgtat taagtaaaaa atgttcagaa gtaccaaaag ataaaataaa ggatatattc
    14401 gggagatttt aatgagtggc tatttaattg taaacttaag taatatgcta ggagagctgg
    14461 aggaagaaga agttaaaaaa attctctcca gtttttcttg tccccttaat aaagatgtag
    14521 aagaattttt aaaaaacaaa gctattgaat tttctaaaca aggtttggct agtacacatt
    14581 tagtgctaac ttcttataaa ggcaagcctg ttatagttgg atatttcact ctagctaata
    14641 agtattttac aataaaaaga aaaacattat caaactcttt agctaggaaa atagtgaagt
    14701 ttggacaata caatgaagaa ctaagaagat atattattgg agcacctttg atagggcaaa
    14761 taggaaagaa ttattcaaat aattataata aattaatcaa gggtgatgaa cttctaaaaa
    14821 ttgcatgtga caagataaaa gcagtacagt tagatatggg tgggaaaata gtgtaccttg
    14881 aatgtgaaga taaacctaaa ttaattgaat tttataagga taatggattt gtagactttg
    14941 gaaaaagaag ccttgataaa gacgaaacag attcgttaga tgggggctat ttagttcaaa
    15001 tgttgaaata tctaaaaaaa taaaagtaca taaaatctaa aatgactatc ttgatagatg
    15061 gtcttttttt atacaataaa ttaaaaggag aataaattat gaatgattat aaagaaaaaa
    15121 ctgaaccaaa attaaatatt ttagtccagt ctaaagttga tattttatgt tctaatagct
    15181 ctattatgaa tggagtagat acttcaattg atataaaact ttctttagat aatagagtta
    15241 tttgcaattt aactaatgaa gatataaaaa aagaaatatc aacatctttt aataagtgtc
    15301 taaaagaaat tgataaaaaa atttgtagca aggaagtaat taataagctg atttgatttc
    15361 ttcaattgaa gaaattagtt gttcttgaac tttttctata attaattcct tatctaaact
    15421 tccagaataa aaaggattta tacgtttaga ttttcttaaa aattcataat atggatttga
    15481 tgaattttcg gtattagtca aagttaggct tattaattta aagttttcat catcttctgg
    15541 aaatattcta aaacatatta tagaaggatt ttttattgaa taggtatcat ttttggtgaa
    15601 ttcaagaaaa cagcactcaa atactcccat aattgctaca atttcaccat ctttaatagt
    15661 atgagttttt tcatatgaag cattaggata atatttcatt atagtttcaa attgagcttc
    15721 gataatactt tttaattcta atgtaatatt tctttgagct tctaggttca tagctctttg
    15781 tatgcttttt tctttttcta gttgttcttc tgcatgtgcc tcagcagtaa tgtttttcaa
    15841 tttatcttca aaattcatag attagcacct cctttcaata aaattttagc ataaatttgt
    15901 gatgaattct gctgttgtcg aacgattgtt gaaggatatt atattctggt gtagaaaagt
    15961 atatttatga gagaggtgat attatggaaa ttgtaaaaaa tgaatctgaa aaatttgaag
    16021 aactaaagaa aaaagcaaag gataaaacta attggagaaa cagactagaa gctgttatag
    16081 aactaaaaga ttatgattgt aaagagagta tagatatagt tactaactta gctctacatg
    16141 atagagtttt taaagtaaag gaagaagctt ttagagtagc acagtctttg aatgtaacaa
    16201 aaagaggaaa ccctatacat ctaggcagaa aagatattgg atataaaaat aaagattttg
    16261 tgaaagtatt tgaaaaaatt aaaagagaat gtaatatgga tgaatttgat ttagatatat
    16321 tcaaagaaaa attcaaagtt gttaatcccg aaatgtatga tgtaatgaag tttgaaaaat
    16381 gcgaccaatt tgaagaatgt gatattgata aatctgagca atttgataaa tggattgaga
    16441 atatgtttaa atctctgcct aaaaagtaaa agaactccaa acgagttctt ttttttattc
    16501 ccaaaacaaa caaataaaga ggtggtgatg tgcaagatgt caaagaaaag gtaaaacaag
    16561 attacttaaa aggaatgaaa caaaaggaaa tagcatcaaa gtatgacatt agcttaaaca
    16621 ctttaaagtc atggataaaa agatacaact ggtcaaagga aaagaaaaag ggtgcaccca
    16681 taaataaaag aggtgcaccc ttttctaata aaaattcagt tggccatggt gctccaaaag
    16741 agaataagaa tgctgaaaag tttggtttct tctcaaaata cttacccaaa gaaactcaag
    16801 acctaattaa tgagataaag aataaagata aatttgatat tctttgggaa cagataacaa
    16861 ttcaatatgc agcaataata agagcgcaaa agataatgta tgttaaagac aaggaagaaa
    16921 tggttaaaga attaaagaaa catgaaagca cagaaaatgg tgagaagata gagtatgaat
    16981 ttcaatttgc atgggatagg caagcatctt ttcttaatgc acagagtagg gctatgagtg
    17041 aacttagaag tttaattaaa cagtatgatg aaatgattca taaggattgg aatttggcta
    17101 cagaggagca gaaaac
//
LOCUS       DAKBNN010000074        17048 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000074 DAKBNN010000000
VERSION     DAKBNN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.074"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(132..656)
                     /locus_tag="OUB02_002040"
     CDS             complement(132..656)
                     /locus_tag="OUB02_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT8885797.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(721..1473)
                     /locus_tag="OUB02_002041"
     CDS             complement(721..1473)
                     /locus_tag="OUB02_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCT8885798.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1504..2802)
                     /locus_tag="OUB02_002042"
     CDS             complement(1504..2802)
                     /locus_tag="OUB02_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HCT8885799.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINVSQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2819..5083)
                     /locus_tag="OUB02_002043"
     CDS             complement(2819..5083)
                     /locus_tag="OUB02_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HCT8885800.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5338..6156)
                     /locus_tag="OUB02_002044"
     CDS             complement(5338..6156)
                     /locus_tag="OUB02_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCT8885801.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6149..6847)
                     /gene="cbiQ"
                     /locus_tag="OUB02_002045"
     CDS             complement(6149..6847)
                     /gene="cbiQ"
                     /locus_tag="OUB02_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HCT8885802.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6850..7134)
                     /locus_tag="OUB02_002046"
     CDS             complement(6850..7134)
                     /locus_tag="OUB02_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8885803.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7124..7876)
                     /locus_tag="OUB02_002047"
     CDS             complement(7124..7876)
                     /locus_tag="OUB02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HCT8885804.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8058..8250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8382..10154)
                     /locus_tag="OUB02_002048"
     CDS             complement(8382..10154)
                     /locus_tag="OUB02_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HCT8885805.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10168..11097)
                     /locus_tag="OUB02_002049"
     CDS             complement(10168..11097)
                     /locus_tag="OUB02_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8885806.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11325..11513
                     /locus_tag="OUB02_002050"
     CDS             11325..11513
                     /locus_tag="OUB02_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8885807.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11506..12207
                     /locus_tag="OUB02_002051"
     CDS             11506..12207
                     /locus_tag="OUB02_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885808.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12209..13132
                     /locus_tag="OUB02_002052"
     CDS             12209..13132
                     /locus_tag="OUB02_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885809.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13289..14212
                     /locus_tag="OUB02_002053"
     CDS             13289..14212
                     /locus_tag="OUB02_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885810.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14205..14942
                     /locus_tag="OUB02_002054"
     CDS             14205..14942
                     /locus_tag="OUB02_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885811.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14953..15726
                     /locus_tag="OUB02_002055"
     CDS             14953..15726
                     /locus_tag="OUB02_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885812.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15742..15894
                     /locus_tag="OUB02_002056"
     CDS             15742..15894
                     /locus_tag="OUB02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885813.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16267..16875)
                     /locus_tag="OUB02_002057"
     CDS             complement(16267..16875)
                     /locus_tag="OUB02_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCT8885814.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFAISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5492 a         2955 c         2152 g         6449 t
ORIGIN      
        1 cacaatacaa ataaattaaa aagtgaaatg tatttgtcta aaaacataat aacatttcac
       61 tttatcctca tttttaagta atatatattt ttaattttta tttataacta aatctagttt
      121 ttatataaat tttagttttt ttctacatct gagtaatcta ttccaaaagt ttctacagta
      181 acagatttca taacttgtgg agtttgtggc ttatcagctg aatctgttct aactgatgct
      241 atttcatcaa ctgtatccat accttctatc actctaccaa aacctgcata ctgaccatct
      301 aagtgtggtg agtttttatg cattatgaaa aattgagaac cagctgaatt aggtgccata
      361 gttctagcca ttgatatgac tcctctttca tgttttaaat tgttagtaaa tccattacca
      421 ctaaattcac cttttattga gtgacctgga ccacccattc ctgtaccatt tgggcatcct
      481 ccttgtatca taaatcctgg tattactcta tgaaatatta ttccattgta atatccttca
      541 tttactaatg atacaaagtt tttaactgta tttggagcta tatttggata taactcaatt
      601 ttgatttctt ttccattttc catttctata gttactatag gatttttatt ttccataatt
      661 tattaccttc cttttcatta gttaaattac ataattacat ttattatatt atactctaaa
      721 ttaaagtcta tcaagtatat tttttcctat ttcatatcct tcttgataaa tagttttaca
      781 aaactcatca tcaaagttaa gcgtgtcttt aggagcaaat tgagtattag gtctaactac
      841 tattatttta tcttcatcat atactttttt aatatcttct ggcaactcat aattatattt
      901 tgtggatatt aaaataattt tatccacatt atccccaatt aatggttcaa ttaatgcact
      961 cctaattaaa cctccatcta atttgaaacc atcataaatt ccttcttgca catctttcac
     1021 aaattgttct ttaaatccaa gagttgcgtt tggatttgat ggtaacaacc cactgtatga
     1081 aatgctgata atggcttcat tacgaggcat ttttgatacg tcaacaatct tttctgatac
     1141 tactttatta tcaacttcaa cataattaac ataaaagttc atttcttcta tattactatc
     1201 atctagttcc cttaggttgt ctatagcagg agaattatcc acagtattat ttactatttt
     1261 tacatttcca ttctggatat cttttatatt tgtccacatt ttctccaagt tatcaacatt
     1321 ccctgtgtac aaataatatc catttattgc tcctattgat gtccctgaaa tcgaataaaa
     1381 tttatttatt cctccatcat acaaggcttt aactactcct gcttgataag ccccttttgc
     1441 tcctccacct tctaaacata atcctaattt catagtctca tctccaaatt gtttatctta
     1501 tatttatatt tcaaaaaact tactaaatgt tttatatata ctatagtggt cctcaacatt
     1561 tcctaattct cttatagaat gcattgctaa aattggactt cccacatcca cagatggtat
     1621 atctatatga gtagaagata ttggacctat agtacttcct cctctttcat ctgacctatt
     1681 aacaaattct tgatactcaa cattagcttc tctacatata tttttataaa tagctataga
     1741 atatccatca ctagtatatg cctgactaac atttatcttt ataaccggac cttttcccat
     1801 tattggttta ttagttgggt catgcttttc tggaatgttt ggatgaatag catgagctaa
     1861 atctgatgat atcataaaag atgaataaat agactcaaag aatccctctc tatccttacc
     1921 cattgatatg caaattctct ccaatatatt taacaacatg tttgaatctg caccttgttt
     1981 agtagagcta ccaacttctt cattgtcaaa tacagccatt acatttacac catgttgacc
     2041 tttagtatta acaagagcat tcaaactagc atgtaccatt gcaagattat cttgtctccc
     2101 aatagatata aattcatcat tagcccctat taaacttcct ttttcaaatt catataaaaa
     2161 taaatcaaaa tctataatat cgtccatatt tacatctaag ttagatgcta tttgctttat
     2221 aagaaaatta tctttttcta atgtatcatt taacatccca actaaaggta acatatcttt
     2281 ttgcttatta tatgaatgac catcatttat acttctattt aaatgtattg ctagatttgg
     2341 tattatacaa attggctttt taaagtctat aagcctttca tttggtttta atatactatc
     2401 accttttaaa actactctac cagctatccc aagaggtcta tccaaccatg tacttaatat
     2461 tgctcctcca tagcattctg tatttaactt caaataagat ttttcagctt ctatttctgc
     2521 atttggttta atactaaatg taggagaatc tgaatgagag cctattattc taaatccatc
     2581 tctttctata tcttctgaat ttactacaaa agctactata gctgataaat tcttagtaac
     2641 ataatacttt ccaccaactc ttagattcca tctttgattc aaaactaatt cttcaaatcc
     2701 atttttatct aagatttcct tagtactctc gactgcatga aaagacgacg gactatcata
     2761 tatgaagtct attaagtttt tagcaaagtt tatactatcc attcctcatt tacctccatt
     2821 ataaaatatt tagtgttgta tattctttat ttattttttt aattccctgt tggtcaaaag
     2881 ctatagtaac atctgtacca attatagaaa ccactgtccc aaccccaaat ttaggatgat
     2941 gtaccttaga accaagtttt atatcatctg gattagtttc tttattagaa tcttttattg
     3001 tagcattaac tttatctgca actttagatt tgcttatagt actcatatac tttttagtat
     3061 atttatctaa catattataa cttgctttag aatagcttag ttcttttttt actttgtaca
     3121 atctttctat acattcttct ggcagttctt ccataaatct tgaagctatt gcaacatttg
     3181 ttttaccata taatgttctc ttctctgtca atgacatata tagctcttct ttagctctag
     3241 ttataccaac ataacaaagt cttctctctt cttctatttg tgaatcactc atagatttta
     3301 tagctcttga tataggaaat aacccttctt ccatacctgt taaaaacaca acagggaact
     3361 ctaaaccttt ggatgtatga attgtcataa gcgaaaccct atcattgtct tcttcatcat
     3421 ttgattcaga tgtaagtgct atacttgtta ggaatgtctc taagtcttta ttttcataag
     3481 tatcatcatt actactttca aactccaaag ctatagaaat aaattctctc aaattatcaa
     3541 ttctgtcttg agcttcttca cctttgcctg ttaaatcttt ttcattttta tttcttatct
     3601 ctacaagctc gtccatatat cctgtagtat ccaagacttt ttctataagt ttgctaacag
     3661 gataaacttc ttttatagtt ctaagagtcc ctataatatc aacaaatcca cttatacttg
     3721 cccttgcctt agtagatata tctgaatttg tttctatatc aattaatact gagtatatac
     3781 tttcctgttt taagttagct ctatcttcaa ttttttctat agttctaagc cctatacctc
     3841 ttctaggaac atttataatc ctttttatag atatatcatc ttgtggattt tgaataactc
     3901 ttaaatacgc caacaaatcc tttatttctt ttctctcata gaattttgta ccaccatata
     3961 tattgtatgg aatttgactt ctatttaaag cgtcctctac aggtcttgcc tgtgcatttg
     4021 ctctataaag tactgcaaaa tccttataag gtctattttc ttttctagct atttgtgcaa
     4081 ttgaatcagc aatgaaatca gcctcttcta tttcgctacc agttaattgt atctttataa
     4141 gttctccttc ttttttttca ctccaaagct tttttctttt tctttctata ttattagata
     4201 tgactttatt agctgcatct aatatcactt gtgtagacct ataattttgt tctaacttta
     4261 caacatgtac attgtcataa tctttctcaa attcaagtat gtttcttata tctgcacctc
     4321 tccatccata aatactttgg tcatcatctc caacaacaca aatattttga tgttctcttg
     4381 ctaataattt tatcaattca tattgagctt tacttgtatc ttggtactca tctaccatta
     4441 tgtatctaaa tctacttctg taatatgcta acacttcatc attagccttg aaaagttcta
     4501 cagttttaag tattaaatca tcaaagtcaa gtgcactatt tctttttaaa cggtcttgat
     4561 atagcgcata aatatctgca atcttaacca ttctattatc tgccatattg atatctttaa
     4621 actgctttgg agtataaagt ttgtcttttg ctcctgaaat tgcacttata actgcttttg
     4681 gttcaaacac tttatcactt aaatttaatt cttttagaca gtctttaacc aatgtaactt
     4741 ggtctgctga atcataaatt acaaaacttc tattgtaacc aatcttattt atatcttttc
     4801 ttagtatacg aacacagcaa gaatggaatg tacttatcca catatcctta gtttcaggac
     4861 ctatattttg ttcaactctc tctctcattt catttgcagc tttgtttgta aatgttatag
     4921 ccaatatatt tggtgcttgt acacctttat cttctataag atatgctatt cttgttgtta
     4981 ataccttagt tttacctgaa ccagcaccag ccagtataag tactggacct tcagtctttt
     5041 ctactgcttc tctttgagca ggatttaatg tatctaaatt catatttctc ctcctgttaa
     5101 cagccataaa tatattttaa tctaaaatat gaattacttc aaattataat tatgtattat
     5161 attaatagat tatttccaat atattacaat taaataacat ttttccatat aatttatttc
     5221 attgtttaat aaaaaaaata accttatgat ttggggaatc ataaggcacc tttaaggggt
     5281 ctaggttaag ggttattata tataaatcta tttttttaaa ataggtttag ggcttaatta
     5341 tttatttgta ttaagttctc tttccttcca atacttatac aaatcttctt ctttcttaaa
     5401 taagggtaaa ttcatatttc tatgtacttt tactaaccat ggggattcta gaccagcttg
     5461 tataatatta ttttcattta taaacacatt ttctgccttt ccttcatcaa tcaaaatccc
     5521 cttatccaat acatatatgt aatcacatat ttcatacatt aaattcatat catgactgga
     5581 tattactatt tttatattat ttttatttaa tccctttatt atatctacaa tagacctagt
     5641 acttactggg tctaaaccag ctgtaggttc atcaagtaat acaatttcat tttccattgc
     5701 tataactgat gctattgcca ctcttttttt ctgtccatag ctaagaaaat gtactggcct
     5761 gtctataaaa tctattccat taacagctac tagagctttg tttattctct ctttaattgt
     5821 tttttcatcc ataccaatgt ttctcattgc aaaggctata tcatcatata cttttgaata
     5881 aaaaatttgt ttttctgggt cttggaacac aatacctaca ttttttctaa ggttgtataa
     5941 acttctctta tcatatttta acttttcttc tttaaaaaga atttctccag atgtaggctt
     6001 taatataccc attaaattca taaataatgt tgattttcct gaaccatttg aacctattat
     6061 acctataaca tttccctttg aaaaatccat attaatgttt aatagtgcat tggtattttt
     6121 ttcatattga tacgttaaat tatttatttt aaacatcatt atcccctact atgtgaaatt
     6181 taccatcata caattttatg tctaatgata tacacatatc atcatacctt gtcataagtc
     6241 ttttgtacag catacttcca agaataccta aggatttata tgaagttttt aaatttatat
     6301 atccaaatct tagctcttgt gacttgtata tttctgaaaa ttcttctaaa aatatgaata
     6361 taaacctata tattaacatt gataactcga caaaagtgtc aggtaaatga agttttttaa
     6421 aaaagaatat taattgatta aaaggtgttg taagtataca aaaatacaca caagtcaaac
     6481 aagacatcgc tctaaataac acatgaatag acatgtcaat agacgccttt gatattccta
     6541 cattaaaact aaaaaattca aaactatata ataaatctgc tttattaaaa gatatattta
     6601 caagagttat tcctatacta agaaataaaa agtacattgg tattctaagt aattttaaat
     6661 aattttttaa atctattcca gccacacaaa caaccaaaat actcattaaa atcataattc
     6721 cacttaaaat aaatatattt tgtattacca tagaagcaat cagaaaaatt actcctatgg
     6781 ctactttttt gtttggattt actttactca atctatttgt atatgcatat ttatcaatta
     6841 taagcatgat taacttctct ttcctttttg aagacctaat atgtatccta ttacaccagc
     6901 ccctaaagct gcttgtgcag agaataataa actttctatt tctccacttg gtggctcata
     6961 tggactgcta aaccatggtt tgtaatttgg atttatctta gtaatttcac tttcagcttg
     7021 accatctgct cctccatatt cagcacctga gttgacaagt aatgggaata taataagagc
     7081 tactactaat aaaagtaata atatgttagt ttttgttttt gttttagcac tcattagctt
     7141 taactccttt atcattattt tctagtatgc ttttttctcc gttttctgta attaaattat
     7201 atacaattac agtaagtata ccttctgcta ttgctattgg tatttgagtc ataaagaaaa
     7261 ttcctaaaaa tttaattgct gaagccatta ccccacctga tgggtctggg aaagccagtg
     7321 ctaattgtat tgaagttatt gtatatgtgg ctaagtctcc tattgctgct gcaagaaata
     7381 ctggaatagc attatttcca tcttttttct ttaaagcctt aaatattagg aatgatacga
     7441 ttggccctat tatcgccatt gaaaaagcat tagctcctaa agtagttatt cctccatgtg
     7501 ctaataacaa tgcttggaat attaaaacta tagttccaag aacaaacatt acgcttggtc
     7561 caaacattat tgctccaaga cctacaccag taggatgtga acaacttcct gttactgaag
     7621 gtatttttag tgctgataat acaaacacaa atgctccaca taaagctaat aatactttct
     7681 ttttagggtc ttgctttact attttaccta ttgattttaa tccgaataag aaaaatggta
     7741 taaatataac tccccatgct atagaccatt ttactggtaa gtaaccttcc attatatgca
     7801 ttgcatttga tgtaactgga gttaaaatga ttaacataag tgcagctata acccctaatt
     7861 taatattctg tttcatctta gtatccctct ctccctttct aataattaaa acataataat
     7921 acatgtctga tttatttaat aaaataaaaa agcctaactc gatggttagg cttctaattt
     7981 tccacaatct aaatacaaac acataaaaca aacatatctg tataattatt ataagaaact
     8041 ttctccatcc ccgaagaaat tactttaata ttttaggcag gtctcctgac ttagcttcat
     8101 cctactcctc ttccttccca tgagatataa aaaatatcat catagtggct aacaaaagtt
     8161 tcgtctgcat cacagtagcg ggggctgtaa aggaatttaa cctttttccc tattaatctt
     8221 gcttaaagaa cctataatat tatatattta atttttaaat tattcttaaa tcattttcta
     8281 tataaatcat tgtatacctt ttctgtgaaa atttctacat tttttataaa ttttattcat
     8341 ttttatattt ttatgtatat aaattctttt ttgtaagttt attaatttct aattatcttc
     8401 tttaaataca ttgaaactac atcagaaaat ctagttatat ctcttgaata tataaaatca
     8461 tttccatata ttaatctttc agcttcaagt tctgattctt ttcctgtaaa tactcctaat
     8521 actaatatat tttgctttct agcttttctc acttcattgg ctgtatctct caaaccaact
     8581 atacccctat aagccttttc tcctcttaat gttctctcac tactttttcc tatctttaca
     8641 tcatttggtc ttccatcgct gagaactatc aggattttat tttcttcatc tctatttatt
     8701 agaccactac atatagattt tattgccaaa ccatctctat tattaccagc acaaaaatat
     8761 tcaaatatat tttcactatt ttttatacta tcatcataat ctttaaatct ttttaatatt
     8821 gtatagtcta aaaaactaga aaatcccatc actctatttg gtatgccaac ttctgttaga
     8881 gcatttgcaa ttatgtaagc ttgaattgcc acatttccct gatttctact ttgagagcca
     8941 cttgcatcca ataatatatc aataacgtat ttcccttttt cattttctat atctttataa
     9001 aatattttat tattattact tctaccaact ctccaagctc tatttgctaa tatagtacca
     9061 caatcagaat atactctact tttagcattt tcttctatta aaattcttga cagactttct
     9121 tttaatctct ttatttgctg tctatacatt ctagcatgtt ctctaaattt tcctaaatta
     9181 ttttcttttt gtcttgttac atattttagt tgaaatacat tattacactc acttctaaga
     9241 actccatctg taaagtgtat tctacaacct tcatgaacat ttctacaatt ttgtgtctca
     9301 atttttttaa tttcaccttt actcaaataa gtttttccat aataatgttc tatttttgaa
     9361 tatatcttat ttgcagtgtc ttcatcaaca tatataactc ggtctgctga attgctacat
     9421 ttagaatcat caaaatcccc tacaccttct acaagcatgg atgaagatat tgaatctata
     9481 tcactttcaa taatattttc ttcttcatca ctataaagtt cttcatacat gaaatcagca
     9541 aatgtatcaa aatttacttc tacactttct acattatttt catcaataag actattttct
     9601 tctccattta atatgtattc aaaataagtt aaatatataa catctaatgc ttttaaaatc
     9661 tccataatat cttgattggt atctatattt cttatatcat taactattct tctagtcata
     9721 ctatctaatg ctgggtgctt gtccatacta taaagtacaa atgtgtattt aattttatcc
     9781 ataatattat cattgtgtat tttggtaaaa cgtgaaacaa tatctttata agcatttctt
     9841 cttatgtctt ctatccctgg cctatcttta atgacctttt cttcaactat tgtatttaat
     9901 atgacttgaa ttagacccag tataatatct ttgttgtatc cttttcttat tctatttgta
     9961 agatattttt taactatatc ccaatctaca tactttcttc tagcacctgc aattattgcg
    10021 aaatatagac ctatttcttt tgaagagtaa tctttttctg atatatctat actttcattg
    10081 taatcaccag ctattgtcca tgataggtta ttagctctat tttcaaattc ataatcatca
    10141 tatatccatt tcataatctc acctatatta aatatgactt attggaaaca catctatact
    10201 ctcccattta ttaggtattc tagttttaat tacatctcca accatttctt tctcatatac
    10261 atcaaaagtc tttcctgtaa gtcccatatt aattgcaaga gttggtttta atcctcttct
    10321 tatagtctta agagatgcca taagaccacg taaatcaata gctttactag atatctctcc
    10381 attttgagac ttcaattgta aatctaaaaa tattcctgca aagtgtatta atttttcttc
    10441 atcagcatct gaaaattcat tttttaaaat catcattaac ttatcttctt ctataggagg
    10501 aatatctatt accataaatc tagataccag tgcttcatta agttcttttg ttcctgcata
    10561 ttcataattc atagtcccaa taaatcttgt tgctggatgt agattgactc tctcataccc
    10621 aggtacatca attattcttc tatagtctaa agctgaatgt accacaacta tagcatcatt
    10681 ttttgccata ttaatttcat caaatacccc aaaacctcca ttaatagcac actcataaac
    10741 tgagcctctt ctaagtttaa cttcattgtc tataaatgta tctgtaccta taagtgttga
    10801 actgtctgta tttatatgaa atgatgtatt ccactgaggt ctattaaata tttcccctag
    10861 attatcagca agtaagtttt ttcctgttgc ctttggtccc gataataata tattttcttc
    10921 ttctaaaata gctgctatac acatagacaa gatatcttta ccataaaaat aagttttaga
    10981 ctttgttact ctgtattcta aatctttttc taatccataa taatttctaa aatacagtac
    11041 atcttcaata agtttttcat ctaccccttg cacccttaga ttatctatta tatccatatt
    11101 tttacccctt ttatataaat agtatttgtt tcatttctta tcaccatttt agcataaaag
    11161 gatataagct aaaattgatt ttttaatctc cccattttaa gtaaaaccat gcaccatttt
    11221 atcactacaa aattaagagt actacttact gatatttctt gattttgttc gtctatacgt
    11281 atataatata tgctgataaa ttatgaggaa aggatgattg aaatatggaa tatatgtctt
    11341 gcccagaagt agcaaaaaaa tggggtattt ctgaaagaag agtacaaaaa ctatgtgaag
    11401 aaagtcgcat accaggagtt tcaaaactcg ggtatatgtg gctgatacca aagaacgcag
    11461 aaaagcctgt tgatggtaga acaaaaagaa gaaaggaagt acaatatgaa tagcgtttta
    11521 attatagatg atgacaaaga actttgtgct cttatgaaaa aatgtgttga gcaagaaaat
    11581 ttatctgcta ttactgcaca ttgtggtgca gaaggtctac atttggtcga taaaaataga
    11641 cacacactct ctcttgtaat tttggacata atgatgccag atatagacgg ttttcaagtt
    11701 ctgaaaaaaa ttagagaaac aagtaatatt ccagtaatta tgcttactgc taaaaatgac
    11761 gaggatgata aagtttccgg actacgttta ggtgctgacg attacctaac aaaaccgttc
    11821 agcataaacg aacttatggc tcgtgtcaat tctcttattc gtcggtatac tatgttaaac
    11881 cctgctttta taacagacac agattgcata ataataaatg gcatgattat tgacaagaca
    11941 aaccgaattg tttctgtcaa taatattcct gttcaactta caagcaaaga atttgattta
    12001 cttgcttttc tggcttctaa taagggacgg gtgtttacca aaaaacagat ttatacacaa
    12061 gtatgggaag aagaatatgc ttttgacgat agtaacatca tgtctttcat cagtaaattg
    12121 agaaaaaaga ttgaaccaga ccccaaccat ccattttata ttttgacagt tcgtggtgtc
    12181 ggttatcgtt ttaataagga ggcttaacat gaatacaagt gtctatctgc ttttgatatt
    12241 ggttcttagc ctgattatta tagcgtacct tgtttctgtc ctgctacgtg tccgaataca
    12301 acttacactt ataaaagatg ctttagagga tataaaaaat ggaaacctta atcgacgtat
    12361 tttaacagat gaaaacgaca ttacaaaaca aatttgctat gatattaacg aaatagcaat
    12421 caatagccaa tttcaactta ttcagcaaaa acaatcagaa caagcctaca aacagctaat
    12481 gacaagtctc tcccatgatg taaaaactcc acttgcttct ttggtcggtt atttggaagc
    12541 agtagaaagt aaaattgttg taggagagga aaaagacgag tatatccatg ttgcgtctaa
    12601 taaggctcat tacttaaaga attttgtaga aaatctattt gaatgggtaa aattggattc
    12661 taaggaacaa gtttttcatt ttgatatttt tgacttgaat gaattatccc gtaatattat
    12721 atctgattgg atacctgttt tagaaagcag tcattttgaa tatgaatttg atataccaga
    12781 aatagagtac ttcttacgta tagacgcaaa tgcatatacc cgtatcatta ataacctatt
    12841 acaaaatatt attacccata gtagtggcaa caaaatgaca ttgagaattt ttgaaaacaa
    12901 agagcaagct caaattataa taacagataa tgggaaaggt atatcttcag acaatctccc
    12961 acatattttt gaaaggttgt accaatgcga ccattcacga gcttcaaagg gaaatggttt
    13021 aggcttggcg attgcaaagg aacttattaa cgctcataag ggaaatatta ccgccaacag
    13081 cactcccggt atgggaacag aatttacaat attgcttcct aaagccctgt aaaagtacga
    13141 ggctttctta ctctaaaaca agataaaagc aagattatgg caaggttttg gcaaggttca
    13201 tgttttataa ttctaattgt agttgatttt gacgttgtat caactatctt ttgtagaaac
    13261 aaatttatta aaagtgagga aattatatat gaatgatttt gtgattgaaa caaagcaact
    13321 tacaaaaatt tatgggaatc aaacagtagt tgacaaagtc aatctccatg taaaaaaagg
    13381 ccgaatatat ggtctgttag gacgcaacgg agctgggaaa accacaatta tgaaaatgat
    13441 tttaggattg acttcaatca cgtatggcga ggtagatgta tttggagaga atattaaggg
    13501 acgtgaaaaa caggtatatc cccgtatcgg tgcaattatc gaaacgccag gattttatcc
    13561 taatctgaca ggaacggaga acttggagat ttttgcaaaa ctgcgtggta cagccgcccc
    13621 taattctgta aaaagagcat tggaaattgt aggcttacct tataaaaata aaaagctgtt
    13681 tagtaaatat tcacttggca tgaaacagcg acttggtatt gccaacgcta tcctgcatga
    13741 cccagaactg ttagttttag atgagccaac caatggtctt gaccctattg gtattgcgga
    13801 gatgagaaag tttattaggg acttgagcgt tgagcatgga aagacaattc ttatttccag
    13861 tcatattctt tcagaaatct cattgttggc tgatgatatt ggaattatcg accacggtgt
    13921 tcttttggaa gaaagtagta tgagtgaact agaaaagaaa aacagtaaat atatcctgct
    13981 acaagtttca gatattccga agacatcact tattttagag cgtcaattta acacgaagga
    14041 ttattccgtt caagatgact acacgttaca cctttataac actaatctgg atatgggtga
    14101 aatcaataaa gcacttgtaa tgcagaatgt aattgttatt agttctcaac tttgtaatga
    14161 tactttagag gactacttta aaaaaatcac aggaggagag ggcattgctt aaacttattc
    14221 aaacagaatt tcttaaactg cgtcgtagga aacttatttg gctgatgttg ttgtctgctc
    14281 tcattatgcc gtttttcgca ctactatact tcaattattt tggtaaaaca ggtgttgagc
    14341 caacaaagtt ttataaatgg tctgcatttg gttatacact ttggattatt ttgcccgttg
    14401 ttttgggtat attttgcacc atgcttatac acgaagaaaa ccagtatgat atgctcaaac
    14461 agctttggat tgtacctatt agtaaaatgg gatacttttt tagtaaattt ttcgttgttt
    14521 tgatttactc tatttgtttt atgctgatta cagccgttgc ttctgtgtta tttagtgtac
    14581 tttctggata tgtcgtattt agctgggcaa gcattttata tttgttgaaa aaatgtctag
    14641 aaattggtgt aatcactgct tttgtaatgt tgccgatttt ggcaatcgct acatctcaaa
    14701 aaggatacat tctcccaatt tgtatcacat tggtatatgc cttttccggt tttgttataa
    14761 tgacaataaa tatgtattta caccctttat ccagtatggg agttattatc atgagaaata
    14821 aagacattcc aggtcttata ttttcacagg aaattaatgt cccattagca tttctttgta
    14881 tttgtatttg gagtattgta tcagtattgt ttgcaaacat tgcactagga agaagaaagt
    14941 gaggtgtaga taatgtggaa attactttgg gctgaatgtc aaaaaattcg tcgttcaaaa
    15001 attgtttgga ttacagtttt tgcatccatt atgatagcag tcatagtttt tgcaggaggg
    15061 caggatgtat ataatgaccc agatttacat tatggcttaa agagcgttta tgacggttca
    15121 cggtatatag acaatgcagg ttggtatatg gacgcagtac aaccttggtc aacttttttt
    15181 gttcttcctg ctgtcattgc cttattagga agctatatta tttgtcgtga agaagaggag
    15241 gacactatta aatccctgcg tcttattccc ataaatgaaa caaatttaac catagcaaaa
    15301 atgattatta cttttatatt cagtatactt ctttatttgt tactctttac tataacattt
    15361 ttgactgaat ccgttttaca tttttccgat ttatcgacaa aactggtttt aagttgcatg
    15421 aaagagtatt ttcttgatgg cataggtgta ttctttgcaa tttctcccat tattgccttg
    15481 gtatcgcgca tgaaaaaagg atattggctt gcattagtat tcacagaagt ttattctatt
    15541 gctggactat ttgcgggtat gtcaaatgtc ttacaaacct tttttcctat tgcggcaata
    15601 tttaatattt ctggatacta tataactact ggagaaaaaa ttatggggag tatcataagt
    15661 ttattacttt gtgcctgcct ttctgcattt atactgaaag ggctaaaaca caatgaaaaa
    15721 aattgaaata tgtatatcaa aatgaaaaag ataaaaaaac tgattattct aattgtattc
    15781 ttgtttcttt ttggcatgtt acactgttat actgaatgga aattcaacaa tgacccacct
    15841 aaaggggtaa tagaaagaat aaatgagtgg actgataaga ttggactgga ttagtaactt
    15901 atgttaatta tagtgaatgt aatattagca agttattgtt tcgtatttta atactataac
    15961 tttaaatatt tggttttaat cttccaaacg aatataaaaa aactgtcaca gaggcaacat
    16021 atttttgaat atttataaag tggtagtttt gattttcaaa gctgccgcct tttcttctat
    16081 ataagcaaac ttatcctcaa caaagtgtaa aacatggtac attttaagaa aaaagtacca
    16141 cagacacaag caataaaaga aacgccgaga acctacaaaa acgtagtgct tatgcagatt
    16201 tacgacgcct tctattttct caaccgtctt aatgaccttg tacaaaagaa atttttctaa
    16261 tattttttat aatcttatct tgtgatgttt tttatgtata aatgacttaa taacaaatcc
    16321 aataaaaatt atggatattg gtataattag aacgaggaat ttataatcat tataatgtga
    16381 cattattctt acccaactct cagaaaaaat atatcctata ctgattaatg aagtattcca
    16441 tatggttatt cccacgatag atgatattgc aaaagttata atattttgac ctgctatacc
    16501 tgctataaaa gatatgtatg ttctacataa agggattaat ctacatacac aaacagaaat
    16561 agaagagtat ttattaaaat aatcttgaga tgcaaatatt cctttctttg cctttggaca
    16621 tatctctatt attttattta tgatagattt acctccccaa tttcccactg tataacaaaa
    16681 tatcgaacca agtatacctg caattatact taaaattaaa attgtcagaa atccaaaatg
    16741 atttcttgca gcaattgctc cacataaagg taaaacaact tcgctaggta atggaaaaca
    16801 agcatactca atcataatta atacaaatac agagattaat ccatattgta aaataaaact
    16861 attgattaca gacacttact tatctatctc ctttcttatt taaaggtttt aagggtacat
    16921 attctaagga ttctcaccct atatttaaag aatcaatctc tttaaactat tatatgtatt
    16981 tacacaaagc atttataaca tactttaaaa atagtacctt aaaataagaa acttaataaa
    17041 taaaaaaa
//
LOCUS       DAKBNN010000075        16580 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000075 DAKBNN010000000
VERSION     DAKBNN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.075"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            12..860
                     /locus_tag="OUB02_002058"
     CDS             12..860
                     /locus_tag="OUB02_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCT8885815.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            954..2876
                     /locus_tag="OUB02_002059"
     CDS             954..2876
                     /locus_tag="OUB02_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT8885816.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            2958..3821
                     /gene="aroE"
                     /locus_tag="OUB02_002060"
     CDS             2958..3821
                     /gene="aroE"
                     /locus_tag="OUB02_002060"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCT8885817.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4021..5238
                     /locus_tag="OUB02_002061"
     CDS             4021..5238
                     /locus_tag="OUB02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8885818.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVIDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5474..7339
                     /locus_tag="OUB02_002062"
     CDS             5474..7339
                     /locus_tag="OUB02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HCT8885819.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWYISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7517..8881)
                     /locus_tag="OUB02_002063"
     CDS             complement(7517..8881)
                     /locus_tag="OUB02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8885820.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPAAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9059..10402)
                     /locus_tag="OUB02_002064"
     CDS             complement(9059..10402)
                     /locus_tag="OUB02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCT8885821.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10475..10576)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10905..11558
                     /locus_tag="OUB02_002065"
     CDS             10905..11558
                     /locus_tag="OUB02_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCT8885822.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11698..12969
                     /gene="brnQ"
                     /locus_tag="OUB02_002066"
     CDS             11698..12969
                     /gene="brnQ"
                     /locus_tag="OUB02_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCT8885823.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13171..14358
                     /locus_tag="OUB02_002067"
     CDS             13171..14358
                     /locus_tag="OUB02_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HCT8885824.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(14588..16573)
                     /locus_tag="OUB02_002068"
     CDS             complement(14588..16573)
                     /locus_tag="OUB02_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885825.1"
                     /translation="MNSDHYKEELTNFFGDEIIIETQNILDGIKENLEGDVVLSLSPL
                     TSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTNKTSAFEMATYLYK
                     IGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGWRKISLDTYMSLLV
                     VLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTIDEIGDGLIFFNTFN
                     KVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDNMIIYIDEIDKKFI
                     LSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKYKFNNIIGNSSIIK
                     DCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFVAINCASLPSELLE
                     SELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQVKLLRVLQEKEIRK
                     IGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDLPPLRKRLEDIPLL
                     LESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYMGSNYLTINDLPQN
                     ISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKLMKFMEYNVTEYEV
                     RNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         6014 a         2051 c         2765 g         5750 t
ORIGIN      
        1 aaatttaagc tatgaataat caaaaaaatc ttaatattcc aattacaatt agttcttata
       61 cattgggaac agaagtttca ttccgtgata gagttcgtat tgcaaaagaa gctgggtttg
      121 atggtattgg attgcgtgct gaaaattata ttgatgcaaa aaatgcaggt gttacagatg
      181 aagaaatgat tgcaattctt aatgaatatg atatgaaagt tacagaagta gaatatataa
      241 ctcaatgggg aagagaagaa gatagaacgg aagctcaaag agaaaaagag caaaatgttt
      301 atcatatggc tcacttattt aatgttaaac atataaattg tggattatta gagatgatac
      361 cagaagaaca aattataact gcattgggag aattatgtga cagagcaggt gaacttatta
      421 taggtcttga gtttatgcca tatagtggtg taccagattt ggctactgca tggcgtgttg
      481 tgaaaggatg taatcgtgaa aatgcaatgc ttatattaga tacttggcac tgggctagag
      541 caaagcagac agcagaggac cttgcacctg ttccagcaga taagatagta tcaattcaag
      601 tgtgtgatgt tttagaaact ccttatgaaa aacttaggga tgaatcacta catgaccgtt
      661 tggctccagg tgaaggatat ggaaacacag aggaatttgt gaaaataata aaagaacaca
      721 atatatcacc aagagtcata ggggcagaag taatatctga ttctctaata tctaaagggt
      781 tatccactgc tgctgaaact gtatttaatg caactaaaaa agttttggat aaagcatggt
      841 ctgaagtatc tccaaaataa acttatataa tataaaaata tacagagtat tataatataa
      901 aacttttaaa tataaaactt ttaaatataa aacttgaaag gaaggaaaaa cttatgaaga
      961 ttagttctat gtttacacct ataagaattg gttcaatgac agttccaaat cgttttgtag
     1021 ttccccctat gggaaacaat tttgcaaaca cagatggtac tttaagtgaa acttctaagg
     1081 cttattatct tgagcgtgct ttaggtggtt ttggattaat aactatagaa tctagtgttg
     1141 tagacaaaaa agcaaaagga ggtcctcgta agccttgttt atatgatgat agcacaatag
     1201 atagttttaa aaatgtaata gatgcttgtc atgatgcagg ttcgaaggta tctatacaat
     1261 tacaacatgc tggttctgaa ggaaatgaaa aagtagcagg acatccatta aaagctgctt
     1321 ctgcaatacc tgcatcaaat ggtcgtaata caccactagc aataacaaca gaagaaatat
     1381 atgaacttat agaatcttat ggagatgctg cattacgtgc acaaaaagct ggtgcagatg
     1441 ctgttgaagt tcactgcgct catggatatc tggttagtag ttttatatca caaagaacaa
     1501 ataagcgtgt agatgagttt ggtggatgtt ttgaaaatag aatgagatta cctcgtctta
     1561 tcattgaaaa tatacgtaaa aaagtaggaa attctctagc aatattatgt cgtataaaca
     1621 gcactgatga tgttccagga ggaattgatg tacacgatag ttcagtaata gcagcatatc
     1681 tagaagattg tggaatagat ggacttcatg tttcacgtag tatacatata catgatgaat
     1741 atatgtgggc tcctacgact ttacatgctg gattcagtgc agaattagtt actgaaataa
     1801 aaaaagctgt tagtatacca gtaataacag ttggacgtta tacagagcca cagtttgctg
     1861 agttaatggt cagacaagga agatgtgatt tagtagcatt cggtagacaa agcttggctg
     1921 acccagaaat gcctaataaa gctaaaaatg gaaaactaga cgaaatgata ccatgtattg
     1981 catgcttaca aggatgtgta ccaaatatgt tccagggcaa acctatagcc tgtcttgcaa
     2041 atcctatatt aggacatgaa gcagagctta agccagctga aattagcaaa gaagttcttg
     2101 ttgttggtgg aggtgttgga ggtatgcttg cagcatgggt atgtgctaaa agaggacata
     2161 atgtaacttt agttgaaaaa tctgaagttc ttggtgggca aatgcgttta gctgcatatc
     2221 ctccaggaaa aggagatatt actaacttag ttagaagtta tatttcaaaa tgtaatcaat
     2281 atggtgtaaa aatatgtact aatacagaag ctacagtaga acttataaaa gagaaatcac
     2341 ctgatgtagt tattatagct actggagcaa ctccacttgt acttcctata ccaggaatca
     2401 atgattcagg attaattcat gctgtagacc ttcttgatgg taaaaaatct tgtgggaaaa
     2461 aagtacttgt tgttggaggt ggaatggttg gatgtgaagt ggcagccttc cttggagaac
     2521 aagaacatga ggttactgtt attgagctta gagaagaagt tggtgcagat gtaatttcag
     2581 aacaccgtaa attccttatg aatgattttg aaaattataa tatacatacc attactggtg
     2641 caaaagtttc taaattttat gatgatggtg tcgactattt acttacagat ggcacagagc
     2701 atgatttaaa aggttttgat tcagtagtac ttgctatggg ttctcgtaat tacgacccat
     2761 taagtgaagt tataaaagaa gttgtaaaag aaacttatat tgttggtgat gcagtaaaag
     2821 ctcgtagggc attagatgca acaaaagaag cttttgaagt agcattaaat atataaataa
     2881 taaataaatt tttctttgta aataactgaa taggctattt gcaaagaaaa tgataatatt
     2941 gggaggagat tatagttatg gaaatttcag gtagaacagg attatttgca ttaataggta
     3001 caccggttgg tcactctaaa tcaccagtga tgtataatta tagttttaag aaattagatt
     3061 tagattatag atatttagcc tttgatatca cagtagataa ggtaaaagag gcacttttag
     3121 caataaaaac ttttaatata aaaggagcca atgttactat gccatgcaaa tctgctgtta
     3181 cagaatatat ggatgaactt tctccagcag ctcgtataat tggagcatgt aatacaattg
     3241 taaatgacaa tggaaagtta gttggtcata taacagatgg agtaggctat gttcgtaatt
     3301 taaaagaaaa tggggtagaa gttaaaggta aaaagattac aattatgggt gctggtggtg
     3361 cagcaactgc tatacaagta caatgtgctt tagatggagc aagagaaata tctatcttta
     3421 atcctaagga tgatttttat aaaagagcag agcaaacagt tgagaatata aaaaaagatg
     3481 taccagaatg tgttgttaat ctatatgatt tagaagatac aaacaaactt tatgaggaga
     3541 tacaatcaag tgatatattg acaaatgcaa ctcttattgg tatgaagccc tatgacaatg
     3601 aaacaaatat aaaagatact agtgttttaa gaaaagactt agtagtaaca gatgtcgttt
     3661 ataatcctaa gaaaacaaaa atgattgaag atgctgaagc taatggatgt aaggctattg
     3721 gtggattagg aatgttatta taccaaggag cagaggcttt taatctatat actggattag
     3781 aaatgcctgt agaagaagtt aatgaattat gctttaaata gataagagta atatcttaaa
     3841 caatgaaaaa acatacgtat ttcaatattt ttaataataa attacaaatt gtcatagtgg
     3901 gttttgcctc atagtccagt taaatttgaa gctttaactg actatacttt aaatccatta
     3961 tggggatgag tttattgtac acaaaaatgt taataaatta acaagaatgg gagaaaaatt
     4021 atgaacaata aaaaatatta tcctactgcc atagcgttgt atttttcata ttttttactt
     4081 ggtattggaa tttcaatttt aggacaatac aaaccagaat tttcatcaat gtggggggct
     4141 aaaacattat cagatggaac attggatgta agtattgttt tagctgttat tgctgcacta
     4201 ggattaggga gacttatatc ttatccattt gcaggaccta tttcagataa gtatggaaga
     4261 aaagtaagtg gtcttatagg aaactttcta catgcaattt tctttgttgg aattgtattt
     4321 tcaccaaatt tttatatagc atatgtattt gcaataattg gaggggcagc aaattcattc
     4381 ttagatacct gtgtaactcc ttcttgtatg gagatatttg ctagtttagg aacaatagca
     4441 aatatgttta ctaaatttac aatagcttta gcacaatttt tgcttccttt tataattgga
     4501 tttgttgcag caaattcaat atcatttaaa gttattttta tagtaacagc gattcttatt
     4561 gtaatagatg caattttgat agctgtgtta ccatttccac cagctaataa tgttattgat
     4621 aacaaaggca aaacagttaa atcagagaag atgaaattta caccaacaag tatagctctt
     4681 gtttgtattg gatttacatg tacatctaca tttgtattat ggttaaattg taatcaggaa
     4741 ttgggtaaat tgtatgggat ggctgaccct acaaaaattc aatcctttta ctcaatggga
     4801 gtaatatgtg cagtattaat aacttcttta cttattaaaa aatatattaa accaataaga
     4861 atattagtta tatatcctat aatagcttta cttatgctat tagttgtata ttttgttcaa
     4921 actccaacta tttgtatgtt agggggattt gtaataggtt attcagcagc aggaggtgtt
     4981 cttcagttaa caacgtcaac tgcaaatgaa atgtttccaa ctaataaagg aaaaataaca
     5041 tctatagtta tgattgcatc tagtatagca aactatgtta ttttaaatat agcaggtatt
     5101 attacaaaat cagggggagt taatggacca aaatatgttg tattgtttaa tgtggcaata
     5161 acatttgttg gaattttact tgcattgttt gtaaacatga gatatgaaaa agaaaaagtg
     5221 tatgattatg atgtctaata ttagtataat atacgcatag agaagtctaa atctaggata
     5281 gatttttatt aaaagtaatt aaaattaata aggatattat ttacagttat aagatagggt
     5341 atttacataa ttaataataa ggatattact agaatttcaa tttctagtaa tatttttttg
     5401 cgtataaatg taaacttgac gaaataagta gtataatcaa tataaattac atcatgtaaa
     5461 ggaggataat ttggtggaga atgtttttaa gcgattgcag gaatttaatg gttatgatgg
     5521 atacaaagaa tcatttgaaa tgaattacct atgtatatat gaaagtatac cgttaagaga
     5581 acaagtagag cttgcaaata atctagtaga tgaaatttta aatatgtata aaagtgaatc
     5641 taatgaaatt tatttattgg aggatagtaa ttctaagtct ttaatttgtt atttcgaaat
     5701 ttttatgaaa aagataaata ctttggtaaa agaaatgatt attgatgaga aatggttata
     5761 taaattaact aaagaattaa tatataagag taaaaaagtt gagtatgtaa aactagggtt
     5821 agttttaagt gagaaatatc taaatgtaga aaacttaaga gaagttgtag atacattttc
     5881 taaatctgga gaatacgtat tttatctgag taatactatt aaaaaacttg aattttacaa
     5941 tacatattta tttaatttat ctaaaaaggc tacaggttca ataaaagtat ttgctattgt
     6001 taatatggaa aatttagatt ctaaaataaa ttcctatcta attgaagatg gttataagga
     6061 tacaaagtac gaacgattat tgatgaacta tattatttca attgtagatt tgaatgaata
     6121 tcttgaaaaa cgagatttag ataaagaaaa gataaataat ttagcacgtc ttatatgcaa
     6181 ttacttgcta agtgtagaat ttaaatatat aggtaataaa ttagaattag tgaatcgatt
     6241 tttacctact gtagtaaatt atgggactaa ctttgaaagt ttatattcaa ttttcctaat
     6301 agctataaac gtcttaaaag atgaaaatat agagtgcaat aagattgaat ttgaaaaaga
     6361 aataaatggt atcttgctca gtgaaaaatg gaaaaatata tattttgaag cattaagaga
     6421 tgctagtgga aaaacagaag atattataaa aatgtcagaa atttatgatg taaacttatc
     6481 ttttgatgac ttgttgccat atctgaatag agatattaga gattttgagg tatattggta
     6541 tatatctaaa aagggaacaa caagttctag attaaaatta ttaaattttt ttgaagagac
     6601 gtttaaaata gatgatttaa ttggaaaaat gaaagatata gaaaaagata aattgactca
     6661 agaatattat gatgatatgt tattttttat tgtgcttaaa ggaagtaaat cactatatcc
     6721 tgaaggaaag aatatatctt taaaaggaat atttggaaat ataaatgaag taagaaaaga
     6781 atccataaat atcttaaaaa gatacagaga gaaactatct cttgaagaat taaaaattgt
     6841 taaagaagcg tatgaaaaag aaaaaaatgt tatattgaag gatgaattaa gaagagtatt
     6901 atatgaaagt aataatttaa aaaaagaatt tgtaaatatt gaaaaaataa aagtagatga
     6961 acatggtaag gatatctatt tgacttcaat agctgttgct ggttcaaggt ttagaaatag
     7021 agaatatctt gaaaaagaat tggagaaaag taagatatac tatttaacta gagagaaaga
     7081 taatctatat gatgaaaaag ctattaaaat agttggtgaa actggatatg taataggcta
     7141 tgtcccaaga aaagaaaact atattttgag caatttatta gatggaggaa aactgctgta
     7201 ttgtagagta actgaatata atttatatga agattgtatc tacgcaaatg tgtacttgag
     7261 ttataaagat gtaattgaaa cagttgaaaa ctctttaaag atggttttag ataagagtag
     7321 aataaaactt ataaactaag acgtttatat attgcttaga tgatgtaatc ttaactgatt
     7381 ggttattttt tctggaatta tggtgtattt tatgagcaat gctaatgaat ataaaataaa
     7441 aaaacaattg ttttgtatat atgatgtata taaatatatt tatatattca tatttacaaa
     7501 acaattgaaa tttatgttat tttataattt taaatttaga tggaatttta acttctgctt
     7561 tatcttttat tttagaagct tgttttttaa ctttatttaa taattcagat tcatctacaa
     7621 ctaatacttt tctttccttc attaaccatt taccatcaca aatagtagag tctacatttt
     7681 cactagtcat agaataaact atatttccaa ttggattatg cattggtaaa caatgaattg
     7741 tatctggatt tagaataatc atatcagctt tttttcctac ttcaagacta ccaatttctt
     7801 tttctagtaa agcacatttt gctccattta tagtagccat ttctaagact tcttcagctg
     7861 gtacgacagt tgggtttaaa gttcttcctt tatgtattag tgatgtaaga tacatgtccc
     7921 tcataaggtc cattctatta tttgaaggag ctccatcagt accaattgaa acaggtattc
     7981 ctttttctaa catttcagga attgatgcaa atcctagaac tactttcatg gctgcagctg
     8041 gattatgaga aacttttaca tcatgtaatc taaataaatc aatttcttta tgagtaagcc
     8101 aaactgtgtg agcagctaaa agatttggtc ctaatacacc tagtttatca agatattcaa
     8161 ctgttccaac tccattgttt tgctttacaa atgaaatttc atcagctatt tcagcaatat
     8221 gcatatgtat acctgtattg tatttatcag ctaattcttt agttctaaca agtaattctt
     8281 cagttgtatt aaaaatagtt cttaatgcaa accatatttt aatcctctca ttagatgtgt
     8341 tattaaactt atggaataag tctaattgag tgttgatttc ctcatcagta gttttttgcc
     8401 atgcttcagg aagaccatct cctgtatcca taactgattt agctaaacag gctctaagtc
     8461 cagttttatc aacagcttca accatagcat ctacatattg accaccagat tctaaaaatg
     8521 tagtgacccc agattttatc aattcaacac aacatgcagt agatgaaatc aatgaatctt
     8581 catagttgaa gctactttcg tatggccaga ttctctctct aagccaagtt aaaagtacaa
     8641 catcatctgc aagaccacgt cctaattgtt gggataagtg aacgtgtgta ttgactaacc
     8701 ctggcattag tatttttcct ttagcatcat atatctcagc gtctttatct acaatattaa
     8761 tatcaaactt accaacattt tttataatat tattttcaat aagtatactt ccagtttcat
     8821 atacctctct atctgcattc atagtaatta gatatgcatt ttttataagt aattgtgaca
     8881 taagtacaac ctcttttcta tatattttta tatctatgta ttatgataat aaattttcta
     8941 tacaaagtta atagcaaaat caatttatac ataaattgat tttgctattt ataaatttaa
     9001 aataaataag attttattct atataattta aaatattgac ctagaactat ttaaaatatt
     9061 aacttagaac taagtttaca actgcaagta aacctataat ccaaagtgta atactgattt
     9121 tatcagtttc tttagctacc acatgcatta ttatatatga aataaatcca aagcataaac
     9181 caatacttat actataagtt aaaggcatca ataaaactgt aacaaaagca ggtacagcta
     9241 ttttgctatc agcaaaatca atctgtttta tgctttcaaa catgaataca ccaaccatta
     9301 caagtgcagg agctgcagcg taagtaggaa ctataccaag taatggtgta aatataagtg
     9361 tagctaagaa taatgctgct gttactattg aagcaagtcc tgttcttgca cctgctgcaa
     9421 taccagaagc agattctcca aatgttgtaa cagtactagt tcctaagaat gagcctatta
     9481 aagtagaaga aacatctgca tataacattc ttcctaaatc ttttatttca ccattctcat
     9541 cttcaagacc catttgttta caacaagtaa taagaaatgc taatgaatca aacatgtcta
     9601 taaacatgaa agagaatata ggtccaatta atgagaattt caatgcacct acaatatcta
     9661 atttaaaagc aactggtgct atactaggag gtgttgagat tattgctgtt ggcaactcca
     9721 caaatccaaa tataacagat attattgttg caactactat acttatcaat attccacctt
     9781 taactttttt atattctagt attgccatta agataagagc aaaaagggct attaaaactg
     9841 ttggtgtaaa atctgcaagt gaaactatag tatctggatt agctattata acacccatat
     9901 tttttaaacc aacaaaagca aggaaaagac ctatacctgc tgaagatgct actgccaatt
     9961 ctctaggaat tgcgtctgca attgtctttc gaagacctgt tactgcgagt gtaaagaaga
    10021 cacatccaga tagaaaaact accccaagac ctgtttgcca tgggattcct gcacctataa
    10081 ccaaagagta tgtgaaaaat gcatttaatc ccataccagg agctagagca aatggtgcat
    10141 ttcctaaaat cgcaaatata agtgtaccaa tggctgtagc tagtatggta actgttataa
    10201 gggcggcttt atccattcca gcttcagata atattgctgg atttacgaaa actatgtatg
    10261 ccattgttaa gaatgtagta actccaccta ttacttctgt tttaaggcta gagccacgtt
    10321 cgctaaattt aaacattttg tctaaaaagc ttaatgatga attatttcca cctggttgta
    10381 cattgttagt gtttggtacc atttttacct ccaaaaaact ttaagatata aacaaaaaaa
    10441 tatatattca tatagataga atagaacata tattcagagt tgacattcta ttcatagaaa
    10501 acccatttaa ggtgagtttg tagaaactat tagaccgtat tactaatatt atatgaacat
    10561 tttctatttg tttttctgtt gtattatatc gtaatattgt attatttaca ataaatataa
    10621 taaaattatt cgtttttttg tcgattttat aaaaaattaa ggttatttat tgaaaaaaat
    10681 tactttttcc aataattgaa tatgtatgag tataatgtca attattctaa cttgttttat
    10741 actattaatg gaagtttatt ataaatgagt ttgtgatata attataaaag tactgaatac
    10801 atggatattt cgtaaaaact ttttaatata tcaaatatgt atattaagtt aatatattaa
    10861 aaaaaataat atcaaaaata atataattta tagtggaggg gaaaatgact aaaattatta
    10921 aagtgcaaaa acctaaattt acaggagaat tagaaattat tgaaaatctt gaagatatac
    10981 ttgaagatat atttaatgat gaaaaaatat ttaataaaat tatagaaaat gaagttataa
    11041 gtgatttaga ttctgtaaga gttagctttg aatcttgtat atttagaaat gtgtcttttg
    11101 aaaattgtaa tttaaaaaag attgatttac ttgatgtggt atttgaaaac tgcaatttat
    11161 caaattcatt ttttgatgga ggaagtatat acagagttga atttaaaaat agtaaattga
    11221 taggagttag atttgatgat tgtatactta aaaacgtttt gttcaaggat gttttaggta
    11281 agtattctaa cttttcattc tctaaattta agatagtttc tatcttagat tcaaattttg
    11341 aaaattctgt atttcaagaa gtaaaaggtg acaatctagt atttgagtat acaaatttaa
    11401 aaaaagcaaa ctttaatgga actaaacttt ctggcataga ttttactact aataatattg
    11461 agggaattga gattggtata gatgatatta gaggagcaat ttttgatgtg tcacaagcaa
    11521 ttgatttaac gaaattgatg ggaattatca taaaatgaaa aaaaattcat aaaacaaatt
    11581 gggaaaacgt taacaatgtg tgaattttta ccagattgac atttttctga aaataaagta
    11641 agattatctt aatattcaaa atattttcaa agggggtaat ttagtgtgag taagcagatg
    11701 agagatattt ttatagttgg ttttgcttta ttttctatgt tctttggtgc aggaaatctt
    11761 atgtttcctc catttttagg gatggaatcc ggcaaagact ggttaattcc tcttattgga
    11821 tttgttgttg cagatgctgg tatagcaatg cttgttatta tagcaacagc aagatgtaag
    11881 ggaagcatgg atgatgtact taggagagct ggtaaagggt tagcaagagt aatgagtgta
    11941 gctgctctag catgtttagc acttcttgta ataccaagaa cttgcgcaac aacatatgaa
    12001 atgggtatta tgcctatatt tggagaccat ggtccagtag ctaaagctgt attttcaata
    12061 atcttctttg ggttaacttt agcatttact attaggtctt ctaaggttat agacataata
    12121 ggtaagtatt taactccagc gttattatta gtattatttg ttttaatagt aaagggtata
    12181 atttctccag caggacctat gagtcctgag catatgatag ataaaaatct atttggtgaa
    12241 ggtatatcac aaggttatca aacaatggac gctctaggtg cagcatctat ggcaactata
    12301 atgttgatgt ctataatagc taaaggatat acatcagata aagaccaaat aggaatgact
    12361 attaaagctg gttttgtagc ctgtgtattt ctagcagtag tatatggtgg tcttacttat
    12421 ttaggtgcaa cagtttcaac tttatatgat actagtatac ctcaagcatc tttattagtt
    12481 gaaataacta gtatattgtt atatggtcaa gtaggaaagg taattttagg tgtaattgtg
    12541 gcacttgcct gtctgactac ttctacaggt ttaacagcat caatatcaac ttattttgaa
    12601 ggaataagta aggcgagtta taaacaactg gttataggta tatgtgtagc tagtatgctt
    12661 atatcaaatt taggtgttga ttctataata gccatatcag ttccaatcct acaaactata
    12721 tatcctgttt tattagcaat agttgttatg gagttggctg gtaaacatat aaagaatgat
    12781 aatgctttta aaggtgcagc atatgttaca ttagtgatta gtttattatc agctataaat
    12841 ggaatgacag gagctgttcc atttatacaa agcttgccac ttgcaggttt gggctttaac
    12901 tgggtaatac cagctatatt gggtggaata gttggtaact ttataaaaag caacaaacaa
    12961 gtagcataat aatttgtaat taatttaatt ttataatata ttaattttta gtactatggg
    13021 taggtttaat ctaaacctaa tctacccata gtacttttta gattggatta taatagcatg
    13081 tatataattt tttataatat aatttttctt tgaagtatga taaaattagt aatagtgata
    13141 agaatgtaca aatttaggag gttttaatta atgcttagaa aaataacagc atttatgcta
    13201 tccatatgtt taattattgg acttacaggt tgtagtttac ttgtaggcaa gaaagataaa
    13261 gagaaaatta tagatgattt taaagttgca gtggtaactc aaccattatc tgaaaataaa
    13321 gtacaataca atatggtgga agagatggca aaagaatatg aggaagagaa taaaattgat
    13381 aaagacaaag atggtcaaac taaagttaaa caaactataa agcatgttgt attgccagag
    13441 aattttactt ctaatataga cagtgctatt aataagatag taaagttggc tgacgataaa
    13501 gaagttcagg ctattgtagt ttcaactgac caagcagggc ttttacctgc tttacaaaag
    13561 gtaaaggaaa agcgtccaga aataataact atttctgccc ctatgggaga tgataaaaac
    13621 caattaagcc aatttgttga tgtgaatcta ggggttagtg cagaggaaag aggaaaagta
    13681 ttagctgaac gttcaaaaga aatgggagca aaagctttta ttcattatgc ctctacagat
    13741 gatttgaaag atgttaatat agccaaaaga ttagaaatga ttaaagagac ttgcaaaaat
    13801 ataggattgc catttgtaca agttaatact ccaaatataa atactgaaga agacaaaaac
    13861 aaagttaaac agtttttaaa tgaagatatt gaaaaacaag ttaaaaaata tggaaaagat
    13921 ataaatgtat ttggtgtaaa tgagtatatg gatgaagtta tattgactaa ggctttagaa
    13981 ttaaagtata tagttgcaga gcaaagcaat ccatcaccaa tacaaacata tcctagtgtt
    14041 atgggtctaa aaatatctga aaaagatgct caaaattatg ataaaataaa cgacatgata
    14101 agtgaaaagg ctaaggcttt tggcatgtca aatagacttg gtggatatcc tatgccaatg
    14161 gatgcttttc taccatcagt agcaatttat cttgctactg aaatggtgaa acaagattta
    14221 acacaagaag atgtatgtga cccagattat ttagaggcgt ttacagaatt aagatttgga
    14281 atcggttcag aatttactcc tttaacagag gtactttata attatcaatc agtaatttta
    14341 agccaattaa tttattaata ttaaggaagt gtctcaaaat gaacttttta gtttatgagg
    14401 cactcttttt ctattaaatc aaatatattt ttatcttttt aacaattaaa aagaagctta
    14461 tccctatatt gagatagctt cgtttttaat ataaataagt tttattattt tataaaaaat
    14521 ttatagaaaa ataaaaaatt ataattatat ataaattttg ataaagtaaa gatgaattac
    14581 tataggttta aattatatta ttttctatta tttttatgcc tttctcagtt attgatgagc
    14641 cttttcttcc tttactagaa attaagtatc cattgcgtgt caaatattct agcatatttc
    14701 ttacttcgta ttctgtgaca ttatattcca taaatttcat taactttgtc ctccccattg
    14761 gtttcatgtg caatgatttt aggatagaaa tgcatatgtt tttgtcatat tggtttaaat
    14821 cattgaatat ggacatatta cttgacatat gattattatt taattttgaa gatatgtttt
    14881 gaggtaaatc atttattgta agataattgg aacccatgta agccatatat tcaatacaat
    14941 ttctcagttc tctaatattc cccatccaag tgtatgaatt taaagcttct aaaagactct
    15001 tatcgagttt tatgttttta tagggaagtt cttttaaaaa tgattctaaa agaagaggta
    15061 tatcttctaa tctctttcta agaggaggta aatctaaagt gaacatactt atcctataaa
    15121 ataaatccat cctaaattta ttttctataa ttaatttctc taaatcttta tttgtggcag
    15181 ctaatattct cacatcaatt ggaataatac ttgtaccacc aatttttcta atttcttttt
    15241 cttgtaagac tcttaaaagt ttaacctgta aatcatgtgg catatctcct atctcatcta
    15301 aaaatatagt gccagtgtga gctaattcga ataatccttt ttttccacct ttttttgctc
    15361 cagtaaaaga accatcttca tatccaaata gttcactttc aagtaattca cttggtaatg
    15421 aagcacaatt tattgctaca aaaggtttat tttttctatt tgaatgattg tgtattgact
    15481 gagtaaaggc ttctttacct gttcctgttt cacctgttat taatatagga ttatctgtaa
    15541 gagccatttt ttttgccttt ttaatacaat cttttataat agaactattt ccaataatat
    15601 tattaaattt atatttagct acataacctc ttttgacaga atcacttctt attttttgtt
    15661 ctaatatttc tatattgttt acatctttca atgtaattaa gcaaccctct atatttttat
    15721 aaagataaaa tggtttttta gaaagtatga attttttatc tatttcgtct atgtatataa
    15781 tcatattatc gatatttaag ttctttgtaa ttttatctaa gaaactattt ggcatatatt
    15841 ccattaagga aggtgatttt ataagttttt catctagtcc caacatatta agtaaagact
    15901 tatttacaaa agtaaccttg ttaaaggtgt tgaaaaatat tagaccatca cctatttcat
    15961 caatggtcat gtaaagtata gtttttaatt tagatatatt atctaatgat gtatttaaga
    16021 aatcatgact tagtgtctct ttggacaatt tgtatagttt ttctataaat ttctcatttt
    16081 ttagttttaa tactactaat aatgacatat atgtatctaa tgagattttt ctccaaccta
    16141 aatcaacaat attttttatg tattttggta taaatctaat ctgacctggt gttatagctg
    16201 tatctaaatc atatatttca tcacaatctg gatatgtcgg tacaaaatct atatgattta
    16261 taccaatttt atatagataa gtagccatct caaatgctga agttttattg gttgtcataa
    16321 gtaacgattt tgtacctgga ggtagtttca tcattttttc atatccaagt tttgaaagtg
    16381 ctttcgttcc atgaataatt tcaatatctt ccttaaaatg ttttattaaa aaattactag
    16441 ttaaaggtga tagagaaagc actacatctc cctctaaatt ttcttttatt ccatcaagta
    16501 tattttgtgt ttctatgatt atttcgtctc caaaaaagtt tgttaattct tctttgtagt
    16561 ggtcactatt caatttatca
//
LOCUS       DAKBNN010000076        16540 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000076 DAKBNN010000000
VERSION     DAKBNN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.076"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(509..919)
                     /locus_tag="OUB02_002069"
     CDS             complement(509..919)
                     /locus_tag="OUB02_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885826.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1135..2517)
                     /locus_tag="OUB02_002070"
     CDS             complement(1135..2517)
                     /locus_tag="OUB02_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HCT8885827.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSSIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2528..2887)
                     /locus_tag="OUB02_002071"
     CDS             complement(2528..2887)
                     /locus_tag="OUB02_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8885828.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3092..3871)
                     /locus_tag="OUB02_002072"
     CDS             complement(3092..3871)
                     /locus_tag="OUB02_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCT8885829.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4332..4943)
                     /locus_tag="OUB02_002073"
     CDS             complement(4332..4943)
                     /locus_tag="OUB02_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8885830.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(5035..6024)
                     /locus_tag="OUB02_002074"
     CDS             complement(5035..6024)
                     /locus_tag="OUB02_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCT8885831.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(6091..6954)
                     /locus_tag="OUB02_002075"
     CDS             complement(6091..6954)
                     /locus_tag="OUB02_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HCT8885832.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(7060..7656)
                     /locus_tag="OUB02_002076"
     CDS             complement(7060..7656)
                     /locus_tag="OUB02_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HCT8885833.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAM
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(7696..8499)
                     /locus_tag="OUB02_002077"
     CDS             complement(7696..8499)
                     /locus_tag="OUB02_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCT8885834.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8535..8975)
                     /locus_tag="OUB02_002078"
     CDS             complement(8535..8975)
                     /locus_tag="OUB02_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCT8885835.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(8988..11090)
                     /locus_tag="OUB02_002079"
     CDS             complement(8988..11090)
                     /locus_tag="OUB02_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCT8885836.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEDNEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11303..13006)
                     /locus_tag="OUB02_002080"
     CDS             complement(11303..13006)
                     /locus_tag="OUB02_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HCT8885837.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(13032..13991)
                     /locus_tag="OUB02_002081"
     CDS             complement(13032..13991)
                     /locus_tag="OUB02_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8885838.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(14127..14645)
                     /locus_tag="OUB02_002082"
     CDS             complement(14127..14645)
                     /locus_tag="OUB02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HCT8885839.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMSLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(14688..15173)
                     /locus_tag="OUB02_002083"
     CDS             complement(14688..15173)
                     /locus_tag="OUB02_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HCT8885840.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(15170..15787)
                     /locus_tag="OUB02_002084"
     CDS             complement(15170..15787)
                     /locus_tag="OUB02_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HCT8885841.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(15852..16214)
                     /locus_tag="OUB02_002085"
     CDS             complement(15852..16214)
                     /locus_tag="OUB02_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCT8885842.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
BASE COUNT         5325 a         2994 c         1605 g         6616 t
ORIGIN      
        1 tgccaataaa ctttgatttt tttaaacaaa tcttaaaaat cctaaaatgc taattttctt
       61 taacttttaa tgaatctaat agataacatt taattttcgt tatatttttc agaatatttc
      121 tattattatt tttaagaaaa aaaaataatg ttaacatttc ttaacataca ttaaaaaatg
      181 ttaacattat aaattgggtt taatactttt actttgactc gatttacttt tattctactt
      241 cttactttat ttttaactta gactttattt ttttattact tatttgagtc ttatttattt
      301 tattctcact ccatatgttc aattacattt gtctaaattc tttgatactt ttattacata
      361 attttaacaa gcttaagtgt tatttgggta gtgcagttga taattattta aataatcatc
      421 aactgcatgt ttatatcatt acagtagctt aatgtattta atcatactta cttaggattt
      481 tgaaatatct tagagattat atttaacatt agtcaattgc tgcataattg taagcaattg
      541 aaatttgaga cccatcttca ttttctatag tgataggctt cataattttt aatccttcag
      601 tttttatctt ttctaattct tgttccattt tcttaattgt ttcctcaagg tctttttgac
      661 ttacatcttt gactgcatta gggtctttag catagtcttt taattcttga atatgtttag
      721 catactcttc ataagtataa tgctcaacac catacttttc caattcagta gggtcagtta
      781 ttcctgattt tattagagat tcaaatttag gtaaagcaat attagatgaa tcatttccat
      841 ttgcaaatac tgctccagtt cctcctagta ccattgcaga taccatcatt gcacttataa
      901 gggatttttt tattttcata accaatcttc ctttcttaga gatttatttg ggtagtgtag
      961 tttaaaatga ctattgcaat agttatcaac tacactctta gactatcaca gtagtcatgt
     1021 ttatgtcaag cctttttaaa aaacttttca ataaaaaata ctatctcaaa gcaaaattaa
     1081 ttattttgag atagtatttt aattctaaat cttttatatt actaagattt atggttattc
     1141 tatccatgtt ctatattgtt ctttattaac tgctaacaaa ttcccttttc ttgatatgac
     1201 aaagcgatta tcattatagc cacttcgctc aattgaaact tctcctgaat cagtataaag
     1261 tacatcctta ctattactag atttatttac atacgtttct tcactattct cagaattagt
     1321 tactttgctc atagatgatt cttcatgctc tctaaattca attacagata cttttgccgt
     1381 actaaaatga gtctctccat cacttttagc tttactaaca agaatatcag attctacttt
     1441 gggagaatat aaatcagttg atttactact tattgaaaat ccaattttat cactttgttc
     1501 attttttgtt aaaccaaaag attcaacctc attaatctct ttattttctg ttgcaaaaac
     1561 aagacctgtc ccacaaacta atacagttga aactaaaaaa gtaacataag ttgatttttt
     1621 aaatttcata atattaacta ttctcttttc tatttttgtc tttccaaatc cactacatat
     1681 tgtcattgaa aatggattat tctcctctgc tagacttatt agtgtggttg cataatcagc
     1741 cctatattct tcaccaagta tactcataac cttttcatca caactaaatt ctatgtcttt
     1801 atttagtaaa aaaaacatca cccaaactaa gggattaaac caatggacaa caagagctac
     1861 aagcataaat aactttaaca gattatcaaa gctattaata tgaaccattt catgagttaa
     1921 aacaaagtct aaagtttgat tattagcata attcatttgc tttggcaaaa taattcgtgg
     1981 ttttaaaata ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa caataaatct
     2041 tctaaaacgg ctattcttac acatccattt tttaatgaac tcatgatttt ttatatgaat
     2101 tgaagtttta aaaagattaa ttattttaaa atatctaaag cataaaagta tgctaaacat
     2161 aatagtaact ataaaagaca aaatgcccaa catttccaac atcataatat tttcaatact
     2221 agacattatt tcaaattcgt cagaattgtt tttacctgta gagtcactag ctttcttaag
     2281 agtataattt tctgttgatg tattacccaa ttccacatca aaagtcgatt caattatact
     2341 ataaatacta aaaccagaat ttaaacttac tggtattaga agtttaaata aaacaatcaa
     2401 ccacaataat acaaatacct ttttaggtaa ttttttaaat aaacatgctc ttataaaaat
     2461 aactattaaa agtaatactc cagaagtaaa actcatttct aacaatttaa tgaacataaa
     2521 tcttacctca ctttaatttt tcaacaattt gtttaagttt tgtaatctca tcacttgaaa
     2581 gattttcttt attaataaaa gctgaaagaa atttttctga agaccctcca aacattttat
     2641 taattaaagt ttctgtttcc tgtttttgta cttcctcttt gcttattaaa gctttacaaa
     2701 caaaattcgg ctcaattctc tctattgcac cttttgacac taattttttt ataacggtat
     2761 acgtagtgtt tctattccat cctgtttctt cttttaaaat ttttactaac tctccagcct
     2821 tgctttctcc tctctcccat agaacttcca ttactttaag ttctgaatca aataatttta
     2881 tctccatata aaatcgctcc tcaaatatag ttatcacaat agtcattact ataataaaaa
     2941 actatcatag tagtctatac tatgtcaatc atttattttt taataataaa aactgtcttt
     3001 ttaaaaaatt cttataaaag acagttttat caatgttata ctttaatgtt taattaaagc
     3061 catattttat ttaaatatat aactatctat cttagtacat agtataagat ttttctttat
     3121 ctaatagtat atcctctaca tctgggtttt cttttactaa ttgtctataa aaattacaat
     3181 tagtaacacc cacatatgga attagccata gtagacctat tccaagagta gctattgaaa
     3241 gtatatacca tcctataaag ctaaagttta gtacaaataa tttaagtaca tttcctttca
     3301 tcatatctaa actagcaccc attgatttaa atacacccat attatccttg tcttcacaaa
     3361 ttaagaaagt agcttgagaa acatataata gcaatatata aactggtaac ataagaacaa
     3421 gtattataag aactagagtt attccagtag aaataatagt ccctattcca acattatcac
     3481 ttgataaata ttcaattgct ccactagtaa gtaaaactcc aaatgataaa aacgctccta
     3541 taattgtgat tataaaagaa taaatataca caataattga ttcaaataaa taaaaaccaa
     3601 aacttcttaa ataaattctc ccacttacaa agatatctcc tatcttgact ttttcatctt
     3661 tagtaatttt caagaacata catgtaaatc ctgctgaaac gcaaaaagct attacagcca
     3721 ctacaatagt agtcaatggt tcaaaaggta atcctacaat tgatattaat actatcaatg
     3781 caatatatac tgccatagaa attgcacata tcccccagtt tccagataac tgagctttag
     3841 atactcgttt taattcttgc cttgatacca taaaaaacac ccctttcatt ttataaaaaa
     3901 acttttaaaa atataaataa tcttttaacg tataatatta atatatcata ttttcgacta
     3961 tataagatac aattattcac catgttttat caagatatgc cataattgta ttactagttt
     4021 tatatagtca acattaccaa atacatcaaa taaatttgat taattattca acaagtaata
     4081 aaataatttc tattaattat actttattta gtaaacaaat tatttaataa aaaaattacc
     4141 taatacagta aattaattgg taagataaat tatttattat tagtaaagta aattatttac
     4201 tatttatcaa gataaattat ttataaaata aatcatttat aaaatataat ttatactttg
     4261 aaaataaaat aaactatctt ccaaacattt tattatatgc ttaaaagata gttttgtgtc
     4321 tttaaaataa attaaattgt atatattcct tcacataact tttgtatatt actaggtggt
     4381 tgccatttac catctttttt agtatatgta atcatagttg taacttcctt agttggtttc
     4441 ttattgtaaa ggtctataat catttttgtc atatcatctt gaacttcttt ttctttttta
     4501 tcgtaattct tttcatcagt tacataagat tttactaaag tgttcgcttc actcattact
     4561 ttactagtat ctaaagcttt acttacaact ttcaaagtta tcttatcctt actagaagat
     4621 tccactgtta ctgtcttttc aacaccttga attaatttaa caattgtttc gtatatcttt
     4681 tcagcatttt ctttaccata aagaggtgtc aattgagcaa tcattgaaac cttgtccttg
     4741 ctgttataag tttgaatctg tttatattca gcttctgtta atgcatcaga aattcctttt
     4801 tcatcatact tatacatttc taatgcagct aaattagctt caaaattcga ctttactttt
     4861 tcttctgcat tagaacaccc tatagttaga actaaaacta gagccataat cgtagataat
     4921 attaactttt ttaccatttt catacaaaca tcctccctca attaaaataa taaaaaattt
     4981 atttatcaac attataacat aaatttgtct aactatagaa ttattttgaa tattttacca
     5041 cttccagtct attccaatac cgttttcttt tgctttatcg tatattcttt ttgctgttac
     5101 gacatcttgt acacctatcc caacagtttt aaatacaatt atctcatcat catcttctct
     5161 tccaggaata gttccgttta taacattccc taaatcacca ctaaaatcat ctttacttat
     5221 cttaccttct tttaatggaa ttaatatatc tcctgactca gataaaactg catctgttga
     5281 ttcaaaaaat atcttagacg ctctagttaa acagtttggg cacaactctt gcatatctgg
     5341 catatatgaa cctactgcac ttattaaagc tccttttttc actaagtttc cattaagtac
     5401 tggttgtttt gatggagtag ctaaaactat cacatctgcg ttatctatgg cttcatcaga
     5461 tgattcaact gcaactattt tagtattata tttacttaat tttaagttca tttcttctgc
     5521 aaatttttct ctactttcct tgtttctact aaaaactctt acctcatcca aatttctagc
     5581 ttctatcatg gcttcaagtt gtggctcacc ttgtcctcct agacctatca aagctccaat
     5641 cttagaatct tttttagcca atacgtctat agctgctcca gaggctgctc cagtacgaat
     5701 ctgagttaca taagtaccat ccaatactgc actaacttct ccagtttctc catctaataa
     5761 tagtactgtt gctggaatta ctggtttgcc tttttgagca tttttaggaa atacagatac
     5821 aatttttatt cccccacagc ctaattcttc tacgtatcct ggcataaata acatactagc
     5881 atcttgactt ggaacagata tattggttct caaaggattt acactctttc cttcacaata
     5941 aattctaaaa gcttccttat cagcttcaat tgcatctctc attgtaaata ctttttttat
     6001 gtcgtctttt tttaaaagta gcatttttat acccccaatt ttaataagtc tctatgctaa
     6061 atattatagc ataacattta atttaatctt ctatttcaaa tcaaataaat ctacatactc
     6121 tggttttagc atagaactta tcaacgcttt atttattgaa aattctggta ttcctgctgc
     6181 atatggagtt atatcatata aatcaaaata tacaactaga ttctcatctt gcaagtaaaa
     6241 gttttgattc tcttttatac ctttaaaatt atatattccg atattttctt catccttttt
     6301 ttcaagttca gctatttgtc ttttaatttc ttcatctata acttctttat atttagtatt
     6361 atccttaaac aagtccatta attctaaaaa ttttccagtt ctcatatcca cattatatgc
     6421 tatatcttga taaaatccat gtgctccacc actataagtg taatatctca cttttatact
     6481 aagagcagaa tcagtattct ttttaagttc aaagtctaca tttgcaacaa acttattttt
     6541 ctcgtcagga ttatccttaa gatactgctt tgcttcttta tatgatttat tataaaattc
     6601 aaatatgtca ttttttatct tatcatttgt agacctttca acaattttat ttgaatttgt
     6661 tattacaggc atgtttatag tactttctaa atactcatca tttttcacta ttgttttact
     6721 tcctattgta cttactgata aagtttgttt actatttgta ttaccctttg gtaatgactg
     6781 gttactcata acagtcactg cttccttatt actatttaaa ttaattgaag catctttcct
     6841 taaattatta tatttatagt catgtttaat tgccaaaaca gccattatag tcaatgttaa
     6901 aacaaataca tatcttaaaa ctttagcttt tgtatcgact ttaaaaaatt tcatattatc
     6961 attttcctcc taaatataaa tctctcttat tatatttgga gtttctaggg ttttttatcc
     7021 atataattta ataaaagatt aatttttatt tataaaaaat taatcttttt gttttaaaat
     7081 attaatatgt cttatcaaac agtttgaaat ttcaccaaga ggtaattgta acataacaga
     7141 acctctatca aaagctacca ttggcattcc atatactata caagaatttt catcctgtcc
     7201 aattgtaaat gaaccatttt ctttcatttt taaaagtcca cttgctccat cataacccat
     7261 acctgtaagt attattccta ttgaattttt acctgctact tctgctactg aatcaaacaa
     7321 aacgtctact gatggacagt gaccacttat tctttctcct ttttcacaag ttacataata
     7381 tccttgttta tccgtacaca atttcatctg gtaaccccca ggtgcaatta acacatttcc
     7441 ttgttctact ctatcattat tcttagcttc tcttacattc ataatacact gtttgtcaag
     7501 ccgttgtgca tacatataag taaacatagc tggcatatgt tgaactacaa caatccctgg
     7561 tgttgatttt ggtaggtctt ttataatttc aagtattgcc tctgtaccac ctacagatgc
     7621 gcctatagca ataaccatat tattaaaacg ttccacatta ccgcctccaa aaaatcatat
     7681 atcaaaaata cctttttatc gtttatatat agcaggtttt acatatttgt atttagtact
     7741 acttctgttg attacttctg catgtcctac aaacaaaaaa ctatcttttt tggtagcatt
     7801 ataaaattta tttataagtt cttctcttgt ttttaaatca aaataaatca taacatttct
     7861 acaaaaaatc aaatcaaaat gttgatatga gaaatcattc attaaattaa acggcttaaa
     7921 aattacactt tttctaatct tatcacaaat tttaaatgta ccatccttgt tgtctataaa
     7981 atacttcttc ttccataact caggaacatt gttaacattt gattctatgt atatacctct
     8041 tttagctttt tcaagtgagt tagttgaaat atctgtggct atgattttaa tgttccacaa
     8101 ctctttatta tatccaaaat aatcatccaa agtcatagct attgaatacg cttcttctcc
     8161 actagaacat cctgcactcc atatatttat gtttcttatt ttattatttt cttcccaaaa
     8221 aggaagtata ctattctgta taaactcaaa atgttgaggt tctctcataa aaaatgtgtg
     8281 gttagtactc aatctattta ttaggtttat agcttcattt cctgtcttat ctttaaataa
     8341 tagattcata tattgactaa aagaactaag ttttttctct atcattgtat tgtataagcg
     8401 accttcaatt aacgctcttt ttttacttaa atcaatacca tattcttttt ttacatggtt
     8461 tacaagaact ataaattctt catctgtgag ttttatcatg caaaaacttc ctcctgctaa
     8521 tttatcagaa ctaactatat gctattaatc aaaaactttc gaatatctaa aattaatata
     8581 gaattatctt caactttcgc aatctctttt atatctttat taatattagt gttgttagaa
     8641 ttagtcgtct ctagtaaatt tagattttct atatgaataa catcaacaac tttatctaca
     8701 ataattccaa cttgatactc atcaaccttt gtaattatta tacaagttct ctcattataa
     8761 tcagctttag gaagattgta ctttatatgc atattaataa ctggtattat tcttcctctc
     8821 aaattaataa ctccacttat atatgttgga agacatggta catacgttat tgattgaaat
     8881 cttatggttt caatcacatt ttctaattct attccataaa taacatcatt gacataaaat
     8941 gttaatactt gtttaataat ttcatcttta gccatacttt aaatccccta atatatttct
     9001 ataatgctat ctgcatctat aattaaactt atacttccat ccccaagtat tgtacatccc
     9061 gataaaccct tattttttat attaaatcgg ttaaaataaa ctggaaatgg tttcacaaca
     9121 acttgctgat tacctattat ttcatcaaca aaaatgcata ctgctttatg ctcattttca
     9181 ataattatca tattaccatt catcagattg gttacttttg tttgaatatt atatatttta
     9241 tgtaacctta taatgggaat acatgctcca tatattgtaa ttaactctgt cttatttcct
     9301 tctgtaatta tttgatttaa gtctgtaagt ttaaatgttc ttcttatgga tgatattggt
     9361 aaagtaaata gtgaatctcc aactttaaac ttcataccct ctactataga aagagttaat
     9421 ggtattttta tagtaaaggt tgtcccctca tcctgtttac tttttacttc aattgaacct
     9481 ccaacttttt caatactttt acgaacaatg tccatgccta ctcctcttcc agaaaattca
     9541 gttatagatt cttttgtaga aaagcctgga gtcataatca aagaattgac ctcgtcatca
     9601 gtatactcat tactttgatg tttaagaaga ccttgttcct tagcttttgc caaaatctta
     9661 tctctcataa tgcctctacc atcatctgaa atacttatca cagcttctcc gcctagattt
     9721 tcagcagata aaactatctt tcccacttct tctttacctt tcgctatacg ctcctcaata
     9781 ggctctattg catggtccat agcatttctt ataaggtgca taaagggttc tgctaaatta
     9841 tcaattacac tcttatctac ctctgtctct gctcctatag ttattaactc tacattttta
     9901 tctaattttt tattcatatc tcgtacaatt ctatgcattt tttgaaatac accagcaatt
     9961 tgaatcatac gcatagacat tactatatct tgcaattcat ctgtaagctt gtgtaattgt
    10021 cttgttgcct tattgaaatt atctatatca acacctgtag aatttacgct atttacaacc
    10081 attgattcca taattacaat ttctccaact atattttgaa gtaagtccaa tttttctaaa
    10141 tttacactca taatactctg cttacttatt ggtaaatgtt cattgtcttc acttagacta
    10201 tctttttcca tatcagtttt ttcatttaaa acatcttctt gtatctcatc ttctgtatag
    10261 ttattttgat aacttttact tactttatta ttatctttta atttattttc tactgcatca
    10321 ttgtttgatg tatttttcat ttctatttct tttttactaa tatttatgta ttcttttaca
    10381 tagagattat cttctatagt tttataaact attgccttat cctctttagc tacaaattgt
    10441 atataaaacc cataactttt aatatactct gtagactctg catttgtttc tacatctttt
    10501 ggataaaaat ctataaattc acagtaatct ctaagttcaa atacaagcat gaaagctctc
    10561 acattttcca tcatgcaacc ttgttcaaag attattttta ctacatcaga gtcttctttg
    10621 tattcaaaat ttgaatctat tacttctttt tcgtctttac tttctaaatt tgcattatcc
    10681 tttgatttca tagaacttga taattccaaa gtactcacat actcttcaat tccttcaata
    10741 aaattagagt aatcagaaga tatacaacct cttgattgta actcatctat atctcttctt
    10801 ataaaatcta atgattgaaa gactaaatca aataataggt cattgtctaa aacttctatt
    10861 ttaccttcac gtatagcata aaacaagtct tctacactat gtgctaaagc agatatattg
    10921 tctaacccca tcatagatgc tgaacctttt atcgtatgca ttattctaaa agtttcatta
    10981 atgttgttat gagtaaatac tttctctttt tctatttgaa tcataatttc atctaattgt
    11041 tcgagtaata aacttgtctc aaatataaac atatctaaca tttgtctcat actcggttca
    11101 atataattca catttatcac ctacgaccta caaataattt tctattatat atgcatctca
    11161 taatttaaat ccttgatgat tctcttatct cactatatgt acctcataat ttaaatacac
    11221 aatcattctc ttatttcaat atatatacat ctcataattt aaatacacga taattctctt
    11281 acttcaatat tcacctcata atttaaaccc atgatgattc tcttacttca ctttccttta
    11341 gatgaaattt ttctactaat tcttttaaaa tttgagcttg agcagacatc tcttctgaag
    11401 ctgctgcact ttcctctgct gttgctgagt tcatttggac tactatggaa atttgctcta
    11461 ttccagataa aatctgtgta attgctactt cttgactatg tacagccgaa gaaataccat
    11521 taatataatt agtagttttt tctacaccat ttactaccaa gtccaatgca tttgcagtct
    11581 ctttagcaat tctgacacca ttttctactg ttttaatagc ttcttctatg agaatagctg
    11641 tattttgagc tgcttttgca cttttaccag ccaaagacct aacttcatct gctacaactg
    11701 caaatcctct accatattga cctgctcttg ctgcttcaac agctgcattt aatgcaagaa
    11761 tattagtttg gaatgctatc tcatctattg ttttgattat ttttgatatt tcactcgatt
    11821 tttcacttat ttcacccatt gatgtcatca tatcatccat acatctactt ccgaaaccaa
    11881 cttgtgatgc agattggtca gatacctcac atgcaagact agcactatct gcacacttct
    11941 taattccatc tgatatctca ttaatcgtgg aagctaattc ttctactgaa ctagcttgtt
    12001 cagtagcacc ctgtgaaaga gcttgtgcac ctgctgcaac ttgttctgaa gcacttgcaa
    12061 cttgctctgc tgataaattt atttcagaaa aatcaccatt aaaagagttt attatacttt
    12121 ctaaagatat tttaattgat ttaaaatcac ctataaattc ttggtctatc tctactctta
    12181 aatctccttt tgcaatacta gttaatgtac tgtctataag gtctatgtaa tttaaaagca
    12241 tactttgcat ctttttgaca gaactcacta atagccctat ttcagattta ctttcttcaa
    12301 cgtctatact tgttcttaag tccccagatg caattcttga aaagtgtttt tctactttat
    12361 taataggaat cactattttt aattttatta ctgtatattc tattattgtc aatattatta
    12421 aaagggaaag tataaatatt gttcttaata ttagactttg taattctttt tttacttcta
    12481 caattttgga atttgaacgg gtaactatat cctcttgaaa ttcatttatt aaagaatcca
    12541 ttttctcttg ggagtttata tagtcactac caaaaattat actttgagct ttactcttat
    12601 cattactttc gactgccttt attgcacttt gttctgttgg tactaaagaa tctgatattt
    12661 cttttgcttc taagatttta tttgactcgc tctttgtaag accaattttt tctaatttgt
    12721 ttaaagctga ttctcttgtt tttccgtata attctttttc ataaatatct ttattctttt
    12781 tttctcctgt aaatacatat cttcttaata catcacttat ctctctagtt gtatctttta
    12841 tatactgact ttgagtcctc aaattattca tatcattaat acttttttct actttttcaa
    12901 atgctttata agatgaaata gaagaaaaaa tccctgctag aacagccaat actataataa
    12961 tcaacaatac aattgctaat ttagaaagct ttacttctct tttcatatct gtactccttc
    13021 ataaagtaag tttatatctt ttttaatcct ttcattgtac tcactacgct acaatctttt
    13081 cctaagtttt taccctcata cattgcttta tctgctaagg atatgttttc ttctaatgat
    13141 atatttgagt ttacttctac tactcctaat gtaatagtgc actttatttt atgttcttta
    13201 tagttgaaat caaactcctt tatctcttta cggattcttt ctgaaagtat cttagcacct
    13261 tctattggag tatttgtgag cattatcaaa aactcttctc caccccatct tgatacagta
    13321 tcaatttgtc tacaattctt ttttattata cttgatatta ctgttaacac gaaatcccct
    13381 gcttcatgcc cataagtatc attgataacc ttaaagtcat caatatcacc aaggataagg
    13441 gatattgatg tttcactata tttattaatt cttctttgct ccataagctg attaattata
    13501 tgtcgcctat tgtaaagacc tgtaagataa tctcttacaa cgagtctatt caatttttca
    13561 agagaagatt ccagttcatt atttatcttt tttaatttat cttgagattt ctttacttca
    13621 agatgagcta taacacgagc ttttaattct gtcatactaa aaggtttcac catatagtca
    13681 attgctccta caccaaatcc ttttactatg tctgtatctg tattttttga agttataaaa
    13741 attataggta tatctgctgt acgagcatta gattttactt ccttacaaaa ctcaaaacca
    13801 gagttatctg gtaaaattat atcaagcaaa ataatgtctg gtataaattc ttcaataaat
    13861 tcttttgcct ctattgcatt aaatgctttt ttaaccttaa aattatcttt tgtaagtgtt
    13921 ttatctatta agtcacacat cagaacacta tcatcaacga ctaaaattgt tccctgttgc
    13981 ttatttttca tatacaaacc tctcttaaac ataatatatt caaattttaa tgatttaaaa
    14041 tttttatact taataattat accactatat tcttaaatgt tctcatcaca atctttttat
    14101 aataacactc caactacatc cctatattac atgatagtgc aatcaaaccc attgattaat
    14161 tcatctatta aaacttcatc tttatctgtc aaaagtttct ctaaatctag taaagtaatt
    14221 attttacctt ctaattctcc aactccactt acaaaataat ccgaatattc tatagtaact
    14281 cttggtggag gtgatatttt acttttttct aagcttacta tatcatcaac tctatcaacc
    14341 acaaatccaa ctaccaagtc ctttatttca gttactacta tatacatgca atttgtagtc
    14401 attaattcat cttttttaag tctcaaactc atatctatta tcggaatgat ttttcctctt
    14461 agatttatta tgcctttaga atatgtaggt agttctggaa taaatataat ttcttgattt
    14521 ccagtaattt ctattacctt ttcaagttca accccaaaat cttgctcatc tactttaaac
    14581 tttagatact taattccttc atcaattagt ccataatcta aatatgggtc atctggaaat
    14641 ctcatttaat acctccctcc taaaatacac tttctatgaa atatatatta taatttcttc
    14701 ttacctttat ttattgaatc tacaagtaat tcacccgtct cagtgtaaaa acgtattgtt
    14761 ctaccataat tcatacctgt gtcctcagct aatattggaa tatttaatcc taaaagagtt
    14821 ttttttactg cctcaacatt cctatgtcct atacttgcaa ttactgaatt gctacttcct
    14881 aaaaacatct gagcacctcc agcaattttt gcttttatat atattctatt tgcacctata
    14941 ttctccatag atttaatgag ctcatttatt cctgtgtcag caaatttaca cttattttca
    15001 atgttaatag catctttact atatggcaac ataacgtgta acattccacc aattccagct
    15061 actttatcta tcaaacatac tcctacacaa gaaccaagtg cataagtaac taaaacattt
    15121 ggtctatagg ctatcttcat atctgcaatg ttcactacta tctctctatt catacttcta
    15181 atcccaacct cacgagtatt tctctaagtg agctcatttc tggaatcatc aaaatgtgac
    15241 tagtaaaata attatcactc attttaaatt tattttctat aaataagact ttgtcaccaa
    15301 catctgaaaa tcttatcata ggcacattta gaacagctcc aaccatatca atacatatat
    15361 ctggaattga aacacttata gtcatgttta acatagatgc aatagcattt acatatgaac
    15421 ttgccataat atttcctatt tccttaattg ctgaaatttc aattgcctca atctcatcta
    15481 aaaaagcttc ttctttatta aaaagagaac ttagcataag ttgagttatt gattcatcca
    15541 acaaaaacat aaacattcca ttgatatcac ctttcatatc gagtaaaata catactactt
    15601 tgttttctgg accaccaagc aaatttgtcg cttcattata ttcaagtatc ttgaccatag
    15661 gtattaatac ttctacattg ctatttatca tggatgcaag tgcagtaatt gcattcccag
    15721 agcctatatt accaatttct ctaagagcat ctatatgaac atcatcaagt tctaaataat
    15781 ttataatata aacccctccc tttattattg catttattta cttttcattg tattagtgta
    15841 tatttacact tttattttaa aacattattt acaactttaa ctaatgtttc tgttttgaag
    15901 ggcttcacta taaaatctaa cgctccaagt tttatggctt ctacaatcat attctcttgt
    15961 cctattgcag agcacattac aaccttagca cttgaatcca acttttttat ttctcttagc
    16021 acctctaaac catctttttt tggcattgta atatcaagca acatcaaatc aggcttttca
    16081 tatttaaata tattacacgc ttcttctcca tcatttgctt ctattaaatt attatagcca
    16141 cttttactca gagcttcttt aattatcatt ctcataaaaa tagcatcatc tactatcaaa
    16201 attttatttt ccataaaact aactcctcca tatgtacaac aataaacgct tttatcttca
    16261 gttattgtct atactatctg actatttttc gctacacaat aaatacattt cattgataaa
    16321 taaaagtaat tatttatgcc atattatact taattctctt aacataattt gtcaacaaaa
    16381 aaacttatat atctattgta atatatgttt ggcgttttga cgagttatta aaaatataat
    16441 tgtataaaac ataaaaagtt atcaaaagta attaaattat aataaaacaa ctttatacct
    16501 tttttatgtt tgtataaaaa aaattttcag attcattaaa
//
LOCUS       DAKBNN010000077        16462 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000077 DAKBNN010000000
VERSION     DAKBNN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.077"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..872)
                     /locus_tag="OUB02_002086"
     CDS             complement(<1..872)
                     /locus_tag="OUB02_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885843.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFARVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENV"
     gene            997..1725
                     /locus_tag="OUB02_002087"
     CDS             997..1725
                     /locus_tag="OUB02_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HCT8885844.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(1926..2417)
                     /locus_tag="OUB02_002088"
     CDS             complement(1926..2417)
                     /locus_tag="OUB02_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885845.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(2428..2871)
                     /locus_tag="OUB02_002089"
     CDS             complement(2428..2871)
                     /locus_tag="OUB02_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885846.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(2861..3700)
                     /locus_tag="OUB02_002090"
     CDS             complement(2861..3700)
                     /locus_tag="OUB02_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885847.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            4470..4916
                     /locus_tag="OUB02_002091"
     CDS             4470..4916
                     /locus_tag="OUB02_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8885848.1"
                     /translation="MIKFEKLNIINNKDMKEILDTWESSVRATHDFLSEEKIISIKPQ
                     VKEGAKYVSNFLCVRDKKGLIKAFMGIHDFKIEMLFVGNYSIGNGIGKRLAEYAIEVL
                     NVNYVDVNEQNQQALGFYKHMGFKVFKKSEFDEQGNPFPILHMKLK"
     gene            complement(5146..5286)
                     /locus_tag="OUB02_002092"
     CDS             complement(5146..5286)
                     /locus_tag="OUB02_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885849.1"
                     /translation="MKNENISYLALGLCFGVILGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(5847..7232)
                     /locus_tag="OUB02_002093"
     CDS             complement(5847..7232)
                     /locus_tag="OUB02_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8885850.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKY
                     TVGSFEYTPII"
     gene            complement(7405..8148)
                     /locus_tag="OUB02_002094"
     CDS             complement(7405..8148)
                     /locus_tag="OUB02_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCT8885851.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(8168..9430)
                     /locus_tag="OUB02_002095"
     CDS             complement(8168..9430)
                     /locus_tag="OUB02_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8885852.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEISEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKGLEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(9614..10612)
                     /locus_tag="OUB02_002096"
     CDS             complement(9614..10612)
                     /locus_tag="OUB02_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCT8885853.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWGGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(10596..12107)
                     /locus_tag="OUB02_002097"
     CDS             complement(10596..12107)
                     /locus_tag="OUB02_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCT8885854.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVESLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKDILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(12161..13123)
                     /locus_tag="OUB02_002098"
     CDS             complement(12161..13123)
                     /locus_tag="OUB02_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCT8885855.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(13171..14061)
                     /gene="rbsK"
                     /locus_tag="OUB02_002099"
     CDS             complement(13171..14061)
                     /gene="rbsK"
                     /locus_tag="OUB02_002099"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCT8885856.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(14103..15116)
                     /locus_tag="OUB02_002100"
     CDS             complement(14103..15116)
                     /locus_tag="OUB02_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT8885857.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(15345..16322)
                     /gene="bioB"
                     /locus_tag="OUB02_002101"
     CDS             complement(15345..16322)
                     /gene="bioB"
                     /locus_tag="OUB02_002101"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCT8885858.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
BASE COUNT         5319 a         2941 c         1623 g         6579 t
ORIGIN      
        1 tatacatttt cttttacatg atcattatta aaattaaatc tagtaaaaaa tataccttca
       61 ttcatattac tttttaacaa aaatggattt tctctataaa atactcttgc aaattcttca
      121 ggtgaattta cactgtatac acttacaaat tcacttttag aaatctcatc aaatacctca
      181 aaatcataca taggttcttt atctgtcact aatagagagt taattctaaa accagtttca
      241 taacacttac ttatgcaaaa agcaatttta catatatagt atccatcatt atcttcatat
      301 aaagcttcac ttatatatct gccttgacct ttatcactta ccacattttt aagtaatgtt
      361 ccatttatat tagtaatatg catatttgca atctcgtcac ttccagaaaa gtactttaaa
      421 ctttctctat cccatgctat aaatctcata gtcatataat ttataaattc aacttcttca
      481 tcaactcttt tacatatttt attgtataat ttttctatag ttaaatctgt tttatagttt
      541 tttataatat caatatactc acatttatct cctggcaata gcttatcata ataaagattc
      601 ttattaccaa aataagatac taaaaataaa gattcatcct tcgttaattc aactctatct
      661 gaaccaaatc ctcccatcaa tctttcttct tccatataag cttcttcttg tgttgggtta
      721 tttaaactca cataatctgc taatcctaat ccctctgcat ctaataaaaa atattgacaa
      781 aagtgattgt tttcatcatc tatccaattt attataagac ctaaacttcc cataagtcta
      841 gaatttgtag cctttgcaaa attaaaattc atatagtcac atcctttaaa ctctaaaata
      901 cttgtttttt aatatataaa ttattataca tatattttaa caacttagat acaatttata
      961 atataatata aggaaatgat taattgaggt ggtcttatga aaaatatagc agcttttttt
     1021 gatatagatg gtactcttta tagagactct cttatggttg aacattttaa gaaattaatt
     1081 aaatacgata taattgacca aaaaaattgg tataaacatg ctagagacac ttttatggat
     1141 tgggataaaa gacaaggtaa ttatgatgat tatctattag aaatatgtga cctttatgtt
     1201 gattcattaa aaggattaga taaaacctgt atcaacttta caagtgacca agtaattaaa
     1261 ttaaaagcag atagagtcta taaatacaca cgttcaagga ttgagtggca tctaaatcaa
     1321 ggacatatag ttatatttat atctggtagc cctggatttc ttgttgaaaa aatggcaaaa
     1381 aaatataatg taaccgattt tctaggaagt gattatgtct ttgaaaataa tatatttaca
     1441 ggcacagtta ttccaatgtg ggattctata agtaagaata atgctataaa cgattttgtt
     1501 gtaaagtatg atttagattt atcaaactcg tatgcctatg gagatactaa tggtgatata
     1561 aatatgctaa aaagagttgg aaatccaata gcaataaatc caacaaagga acttttatct
     1621 caaatatcac ttgatgagga aatttctaaa aatgttgaga taatagtaga aagaaaagat
     1681 attatttatt ctctatctac agatgtaaat atagtggata tttaatttat actatctatt
     1741 ttaaaaactt atattatatc taatatttat atctaacata taaaagcttt gactaaaaat
     1801 caaagctttt atatgttgca ctaagaatat attaaattta gctttatgtt ttaatgtata
     1861 ttcatatata taatttacct tatccaaact acaatttcat taaatttaaa gataaaaata
     1921 agcaattatt ttttaaatag catctttctt aacctattat tgtaataacc tataaaagaa
     1981 ctgtaaacat agtcatatac tataaaggct atctcaaatg aacctataat aaatatgttt
     2041 attggaatat aaactattgt tcttaataaa aaatacaata taagtatcat tatatttgca
     2101 aatactaatt tcataaaata ctcaatatat atagacctat ctttttctat aagatatttt
     2161 acaataccat atattccaaa ggtaagtgaa tatacaatcc attgagactt acttgccatc
     2221 actataaatc ctaaaactat agaacctata taaaaagcga ttccactctt aaagccccat
     2281 tccattatta caattgatac taaaagtgat gctaacccca taataaacaa tgtatttaca
     2341 ggaataatat ttgtaagagt caacaatatc acatttaatg ctaataaaac tcctccataa
     2401 gcaactttta tactcatatt tatcatccta acaacaagtt attaaatctc caccaaaaca
     2461 ttcacaacat gaatctaaac accaaagctt agcacaattt tcacaacaat cgtcacctag
     2521 acaacaacag caatcatcac agcaacagca acaacatcca tctaaatcat tacgtcttct
     2581 tctattataa ttgtcagccc tattgctata atcatttcta tattgtgtat aacttctaag
     2641 tgcatcacta tattcactat tttcaggctc cataaatttt gctcttttta agaagtcttc
     2701 accttcttca taatatccta tgttcattgc agaaaaacca gttaaataat accactctgc
     2761 acatttgtca gtcaaagttt ttaaaaaatc atatgctttt ttaaattcac tattttctat
     2821 gaatgatctt gctctcataa aattttcatt attataagta ttactgtaca ttctttccac
     2881 ctctactttc taatatatta atatatctta aataaacacc agaataaatt atattttcta
     2941 ttattccaac atttttattt agatttagat tatcaattcc cctagctaaa aatcctaatg
     3001 atgttactat tatttttttt actttttctt ttaactcatc atttttatca atatactcta
     3061 taaatggatt atatcttcct ttcttaaaat cttcatctaa atcttcatag gcatctagta
     3121 gatatatata cttacctata ttaaaaccta tcattcttaa gtttctctca ttttcatctt
     3181 ttttatagac aaatacttca cccattatat ttccaaatat actagatact tcatctatat
     3241 ttatattttt atctttttct aacttattta attcatttag ttgttgtttt atatactctg
     3301 ctttttcagg atacttttca tatgctaatt ttaatttgtt tttatatata taatatgcca
     3361 atttatcttt tatgcgttta tcatccatta aattatcttc caatttataa tacgttaaaa
     3421 gaatattcat acttgctgca tattctgtaa tatcatttgt aattaccttt ttctttttaa
     3481 atggatttac tatacacact tcctctgtac ttatagactc tgggttatat actgctgtta
     3541 ataaaacaat taaaaatgtt atatcatagt tcaaagaaaa cctagagatt tctccatgat
     3601 ttctttttaa gtatttacat agtccacaat agtaagcttt gtaataatca tattctctaa
     3661 atgttaaatc cattttgttt attttaacat atccaaacat aaatactcct ttttgtacta
     3721 ctttattata taaattatat ttattttcta actcatttat tttatagtgc attatgtaag
     3781 tttttactct ttttacttac tgatgctata tagtcctttt gacttactac atattatctc
     3841 taagttactt atatcattta atagtatatt ctttgccttt gaacaagtca ttttttatcc
     3901 cttattattt aaggttcaat caagatttaa caagctatat atttacattt tttgtaagtc
     3961 taacataatt aatatattaa aaagatgtat ttatcctata tgtttatgtg ttaattagac
     4021 tttagtaacc ttaattgaat ctattgtttc attggttaga aaatgagtgt atgccatcaa
     4081 acattaactc gctcctcttt tattttttgt taattattat tatatcatta ataaaattat
     4141 aattcattta ttttatatgt aatcattaat aaattcttat ctacttctct tttctttttt
     4201 aagtcttcta tattcgtcta agaatgttct gttaggtata gttacttaat tacaatctca
     4261 tctattgtta tacttacatc tttattttgc tataagctat catttattac ctatttcttc
     4321 ttattttttc tattttagtg gacatttaat ttatgacaaa taaatatatt tatttgatta
     4381 tatttaatac aaaactattc ttataacctt tttaaatcat atttaacata taatattaag
     4441 taacatactt acttaagagg aattattaaa tgataaaatt tgaaaagtta aatattatta
     4501 acaataaaga tatgaaagaa atactagata catgggaatc atcagtaaga gcaacacatg
     4561 actttttaag tgaagaaaaa ataatatcaa taaagcctca agttaaagaa ggtgctaaat
     4621 atgttagtaa ttttttatgt gtaagagaca aaaaaggtct tataaaagct tttatgggaa
     4681 tacatgattt taaaattgaa atgttatttg taggtaatta tagtatagga aatggaattg
     4741 gaaaaagact tgcagaatat gcaatagaag tattaaatgt aaattatgta gatgtgaatg
     4801 agcaaaatca acaagcatta ggcttttata aacatatggg atttaaagtc tttaaaaaat
     4861 ctgagttcga tgaacaaggt aatccattcc ctatactgca tatgaaatta aaataacaat
     4921 ataaactata gtataactaa ataaatataa atagagtaat aattgaaaaa tattactcta
     4981 ttatcataac cttactctca cttatcttgt tataataatt atataatata tttttaggtc
     5041 gtttaaactg tatttttagt ttttaaataa caaatatttt ctattgttga tttccaatga
     5101 ttttatacaa aaatagtata attgttatat gttactatag aagttttatt tatttttctt
     5161 actgtctaat actactccaa taactaatcc aaaggataaa ccaatagcta agtttttaaa
     5221 taatgaaccc aaaataacac caaaacataa tcctaacgcc aaataactta tattttcatt
     5281 tttcatatat tactccttta aatttaattg taacatttta acttttctaa ttgattcttt
     5341 tgaagttttg actatatcaa ctaaacctct tatctcatag ttattacact cacttagcac
     5401 tccttttact aatattttaa gatatctccc ctaatttata aacagtagta gtaaaaaatg
     5461 gtttttaaac atatattttc aaagtctaat atatataaaa ctatttttat gaatctttac
     5521 taattccaac tgctcttaaa ggaccgagta acgacattct aacaagtatc agtaccattt
     5581 cttcagggta atctataacc taactaaacc taaaaaataa aattaattat aaacaaaaaa
     5641 taaatataat aaaagggctt acaaattata agtcctttta tcgaacataa aatcaaatta
     5701 ttttccttaa ataggaaact ttaaaaatat attctaatta ttatatctta gattgaaaat
     5761 aattagaaag ttaagttaaa aataatgtta atttatatat ttatcctaat tcatacaatt
     5821 caatcttact tgtattaaat tactcttcaa ataattggag tatattcaaa actacctaca
     5881 gtatacttca attctttttt tgctttattt ataaccaatt catcaagcat tacatcattt
     5941 attgaacatg atatagattt agatgccaat aacattgctt taaatcccat atcagaacct
     6001 gcacttgcac aagattgcca tgtatgggca gaaataccaa taggccaaac tgaacaagaa
     6061 cactgtgctg taggaattat gtaactaaca tctccaatat ccaatgataa tgatatacag
     6121 ttattatgag taaatgtatc atctgtaacc tcatcatgta aattcatctt agtaatatcc
     6181 ttatttccac ttaatacact agaaacacta cttcttttat ctatagacac tgtctcagct
     6241 aatttatctg caaaatagta gtcatcttca ttatagtttt gtacacctac taatttcata
     6301 ttttgtgata gaatatctgt taatattgta tttggaatat agtcatatac tccacttata
     6361 atgtcatatt ccataatggt accagtcatt aatgctgctc cttttgctat atcaacaatc
     6421 ctaccaaata catctcttac atgctccatt ttaactcctc ttatgtaata ccacacttct
     6481 gcaaaatcag gaactatatt tggcattttt ccaccattag tagttacata gtgtactcta
     6541 caattatcaa atatatgttc tcttaaataa ttacaaccta tattcataag ttctactgca
     6601 tcaagtgcac ttcttccgtt ataaggagct tgtgctgcat gagcactaat cccattatat
     6661 ctaaatttca cagagtaatt agcaagtgtt gggattctta taggagtatt tatatcaaat
     6721 ggatgccaac taaaagcaca atcaattcca tcaaaaaaac ccttttttat catcaatact
     6781 tttccagata aatcttcctc tgctggacat ccatagtatt ttatagtccc tttcagcttt
     6841 tttagtttga ttaattcctt tactgcaatt acagctccta ttgaacctgt accaagtaag
     6901 ttatgtccac atccatgacc tgcaccacca tctacaatag gttctttagt agtggatact
     6961 ttttgagaca aaccatctaa agcatcatat tctcccaaaa ctgctataac tggtctacct
     7021 tctccataac tagccacaaa tgctgtatcc ataccttcta atccctctat actaaaacca
     7081 tgttttttta aacattcttt ttgtaaattt gaagcatagt attctttata ctgaagctct
     7141 ggatttttcc atatcttatc tgatatatct cttaattctc cctgtatctc atctatatat
     7201 tccatcaaaa actttttatt taaattatcc atattaatta tataaacctc cctgtttcta
     7261 ataacttata gattatcagc caaaatgact aattttaact gatttaaaaa tattttctac
     7321 catacaacca ttttatacaa taaaacctct tgacttgtaa gtttcatttt atcaagtaag
     7381 aggttttcaa gttttataaa atttttagca tgttaaccaa tcaagcatat taacccataa
     7441 tttattatag tatttccaat cagtaaattc ttttggaccc cagtgaggtg cacagtcaga
     7501 actaaagata gcagattttc cttttccaaa ctcgccaaca gctacaaatg ggtctcctcc
     7561 aatagttgct aaaacaggac aattatctct agctactgtt ttgttatatc ctaaaaactt
     7621 tggccattct gttggtatac cttttaatac tggatgttca gagtcaataa cttctggaat
     7681 tattcctgct ggtttttcaa ctctatcatc tttatctatc attgttattg gtaatacatc
     7741 tttgatagca gtttctccga atctagcttt tgcatctatt cctgtaaatg acatatatcc
     7801 acctaccatg attaacgccc caccattatt tacatactct ttgattagtt caagtctatc
     7861 tggattacta ttgcaatcta taaaagtact attagataat aagaacgtat tagaacctat
     7921 atcagataat attatacagt catactcatt taactcttct aatttatacg gaaaactatc
     7981 tgctgctaca tgtgcaggca tatgaactgt ctcatatcct ccagattcta ttgcttcttt
     8041 caaccacttt acagcttctt cataatgagt tgtaacaaac gtatcaaatc cttttatatg
     8101 agttatattt tttacccaag attcccctac tattaatact tttttcattt atatattcct
     8161 cctactttta taagtttaat attagacttg catacataag tatactatct aaatactcct
     8221 taatttcaac atattcatca attgaatgac attgttctat tgaacctggc cctaatataa
     8281 gtgttggtat ttcagctaaa tttttcatta ctctcgcatc attgccagct gttgaacctt
     8341 ttatctctaa tttttttcct agagtttctt ctagtatttt atgtgcattc actacaaaat
     8401 cagaatcctt gtccatctca aaacctaacc ctgattgata tatgtttata tttggtttat
     8461 tatctttaag ccataaatct ccattagccc ttgtcattat gacatttgtt acatcattta
     8521 caacttgttc ataactcatt atatttggca agaagtgtac acataaatta aatacacact
     8581 tatctggcac agtagaacct gctgttccac catttattac acctaaattt attgtaggtg
     8641 aaggtaatag tggatgttta tatatcataa gccagttatg ttccaaccct tttatatctt
     8701 gaagcaacaa catagccttc tcaattgcat ttacaccctc ccacttacta ccacagtgaa
     8761 gagacacacc tttaacctca acttcaaaga atacaaatcc catatgtgca actattaagt
     8821 tttgttctga tggttcacaa attatgcaac aatctccacc aataccattc ataactgcat
     8881 ttatggtacc atttccgcca ccttcttcat ctactactga cattattttt acattaccag
     8941 gtacatttag accactatct tttataagct ttacagctaa tattgatgct ataagtccag
     9001 atttcatatc agcagttcca agtccataaa gttttccatt atcttctgta gcacgatatg
     9061 gattatattt ccacttactt atatcacccg gtggcatagt gtctacatga ccattaaata
     9121 caattgtctt accaggtaaa tcatcactat acttacaaat taaattatat ctatcttgat
     9181 aattgtgacc tagatttcct tctttatata tattttttgc ttttttaatt aattcttcgc
     9241 ttatttcttg tctatctaca ctaaaaccta tgtaatttgc aagtttttca atatattcct
     9301 gtccttcttt ttcaaatcca cctagtattc catgaccaac atcttcagtc tttatactta
     9361 caagttcttt taaataatct atgtattctt gttttttact atttaatgta tctaagagaa
     9421 tttttttcat atttcctcct aagatttatt tttacattta aaatttcaag tctaatctca
     9481 tctatttaag ccatagtaaa ctttttcact tagcatattc ccattcaatg tataaagatg
     9541 tatgcaaatt aaagtataaa aatatataca aaaactcaat aatttttata gtttataagc
     9601 taaaatttaa ttactattta gatttagctg atttttttaa ttttatatta tttatccata
     9661 tagcaaataa cattaccaaa ccttttataa tcatttgatt atatgaagat acacttaata
     9721 agtttaatcc atttcctaat attcctatta ttccgacacc tatacatgta cctactatag
     9781 aaccagaacc acccatcata ttagtaccac caagcacaac ggctgctata gcatccaatt
     9841 ctgctccatc tccaacagtt ggtgaacctg atgccaaacg tgatgatagt attatcccag
     9901 ctaatgctgt tagtagtcca gctagtgcgt atacttttac tataacttta tttactttta
     9961 ctccacataa tctagcggcc tcttcatttc cacctactgc atgtacatga cgtccaaaat
    10021 tagttctttt taatagaaaa tgtcctgcta ttacaactat aatcataatt ataactggta
    10081 ttggtattgg tccaatatat cctctaccga gcatcttaaa tgcatctgta tttactgata
    10141 taggtcttcc tcctgtaaat acaaagcttg ccccacttat tatcgttaac atggctaaag
    10201 ttacaatgaa tggaggtagt ttttgtgttg aaataattgc accttttatg actcctaata
    10261 ttgtccctat tgctaatgca agtatcatac ctccccatat aggtgcatta taattcacta
    10321 ctgttgtagc ccaaaatgta cctactaaag caattactgc tcctacactt aaatctattc
    10381 ctcctgtaat tattacaaat gtcataccta ctgcaagtat tgcatttatt gatatttgtc
    10441 ttccaacatt aattaaattg tctaaagtta aaaagttaga tgaagctaca ctcattatta
    10501 taaaaattat tacaagtcct acagctgcac tcccttggtc tctaaataca cttgttaatt
    10561 ttgtcaataa tggagaagat ttttcttctt tatgcttagt tattgaactc attaatttta
    10621 cctccgaaag ccaattttaa tacattattt tcaaccattt cttcaccttc taaaaatcca
    10681 cttatatgac cttctctcat aaccattatt ctgtcactca ttgatattaa ttctggtatt
    10741 tcagaagata tcattattat acttacacca ctctccacta aatctcccat tagattgtat
    10801 atctctgcct tagcatttac atctatacct ctggttggtt catccataat taatatatct
    10861 ggttgtaata aaagccatct tgctattata gctttttgtt ggtttccacc acttaaactt
    10921 tgtataactt gattcatatg tggtgttttt atctttaaag cttctatgta tttttctaaa
    10981 atatcttttt ccttttttct atctataatt gatttaaact tagatatctt ttttaaccca
    11041 gccatagttg tgttttcttt gacactcatt tgtaaaaaca atccagtacc ttttctatct
    11101 tctgttatta atcctagtcc attttctatt gcatctgatg gagttttaaa ctttacttct
    11161 tcattgttta aatatatttt cccagaatca tatgttctaa gtccaaatat agcttctact
    11221 atttcacttc tcttacttcc aactagtcca cctattccca atatttcacc ttttcttata
    11281 ttaaagctaa tatcattaaa aacattgttc ttagttagac tttccactcg cataatttct
    11341 tcttttattt ctacttttct ttttgggaaa aattggtcta gctttctatc tatcattaaa
    11401 ttaactaatt cttcctcaga cgatacatct ttggtattca aagtcgaaat cgttttccca
    11461 tctcgcatta tagttattct gtcacaaatc tcaaagagtt cttccatcct atgggatata
    11521 tatattatac taactccact ctcttttaaa tcatttataa gtctaaaaag tatttcagtc
    11581 tcttttttac ttattgaaga agtaggttca tccattatta atactttagt ttctgatgat
    11641 aaaactcttg ctatttctac catttgtttt tgagctatac taagctcttt gactaagcta
    11701 tttggattta tatctattcc catattatcg aaaatctttt tagcctcttc attcattttt
    11761 ttgtcattgg ttaagacttt gccatttatt tcttttccca taaaaatatt ttgtgctact
    11821 gttaattcgt caaaaactgt tagttcttga tatatggcat ttatacctat ctttttagag
    11881 atatctgggg atatattctc tatttctttg ccttcaaaaa taaattttcc agaagttttc
    11941 gaatgtgccc cagttaaaat ctttatcatt gttgatttac cagcaccatt ttcacccagt
    12001 agtgcatgaa cttcgccatg aaataactca aaattaacat tatctaatgc tctgacacca
    12061 ggaaattctt tagcaatatt acttaacttc agaattatat tactcataat gacactcctt
    12121 tttaaatagg atttctttca aataatctca ttttatttta ctaattctta aagtctttta
    12181 tattttcctt atcataaagt aaagctggta ctggtatatt cttttctacc ttttcccctt
    12241 ttatagcttt tactactgct tctacagcta aacgtcccat ctctttaggt tgttgttgaa
    12301 tcatagcaag aatagttcca tctgttattc cacttgatgc ttcttcagtt aagtcaaatc
    12361 ctacaatctt aacatcctta ttttttgcag attcaagtgc agctttagct cctagtacac
    12421 tattttcagc tgttgcatat attatatcta catctggatt agattgaatt aagttttcta
    12481 ctgtattaag agattcttct ctgtcatatc ctggttgtga atttaatatc ttaacatttg
    12541 ctgaaccttt taatgcatct ttaaatccat ctattctctg catttgtatt tgagatttta
    12601 aatctgtcac tattgctatt tctgactttc cacctaactt ttcatctatg tattttttag
    12661 tgtattcccc taatttttta ccagcatcat aactaactgt tcctacataa cttgtaatat
    12721 tttcagtatt taatacagca tctactgtaa ctactggcat tttttcaaat gctccttcta
    12781 ctgctggtac tatagccgaa ttatcagttc caagtactat cattccatct acattttgtt
    12841 gtataaaatc ttctattgct gatgtttgct tagctacatc ttggtctggg tcatttacta
    12901 taactttcac acctaattct ttagcttttt cttttattcc ctcatcaata tctataaaaa
    12961 attgatgttg aagagttatt gtagatactc caatagttaa atctttcttg tctgaaccac
    13021 ctttattatc tgatttactt ggtccttcca ttgaacaccc tgttaacatt cctattgcta
    13081 gtattccagc aattcctaaa gataaaattt ttctcactct cataattaac ctccattttt
    13141 aatagtttta aatttgaaca aattagattt ttatttatta tttaaatatt tttcaacatc
    13201 ttctttatag ggcataccag attgtgcacc tagtttagtt acagaaagac ctgatactac
    13261 tactgcatat tctactgctg tatataaatt tttcccttct gttatagcca ctgccaatgc
    13321 tccattaaat gtatctcctg ctcctgtggt atcttgaaca tcaacactta tacaagatac
    13381 atgcttgatt tcctcaccat cataaaatct agctccattt tttccttctg ttattacaag
    13441 tttatttgga tattttttta atacttcttc tatcccttca tttgtgtcaa aaactatctt
    13501 atattcatgt tcatttggtg ttaagtaagt tactttttct attatatctt catttagttt
    13561 tactgctggt gctggattta ataaaacctt aatcctattt tcaaaacaga aattcactac
    13621 ataatttatt gtttttaatg gaatctctaa ttgtagcaat actatatctg cattctttat
    13681 ctcttcttca ttctttttaa ttaattctat atctactagc tcattcgtcc ctggtacaac
    13741 aacaatacta ttatcatttt cacataatgt tataacagca acaccagaac ttttgtgatt
    13801 atttctggat actaaactat aatcaacttt attcgttcta aaatttctta ttaactcctc
    13861 tcctaagcca tcttccccta tgcatgatat catttttaca tttgctccta gtttagaagc
    13921 tgagatagct tgattagccc cttttcctcc aggtgatgta aaaaaactat tacctaatac
    13981 tgtttctcct ttttctggtc ttttatctac agaacatacc atgtccatgt ttacacttcc
    14041 tatgacaact atgtttccca ttacattact cctttacata tctatttatg tctctcgtgc
    14101 tgttattcac tttcttttgt tgaatctctt atgactaatt ctggtttgaa aatcaattca
    14161 tcaaacgatt cttttttatc tcctcttaaa caatttatca atatttcact ggctttatat
    14221 cctatatcat aagatggctg ttttactgtg gtaagagaag ggcttattag acttgatgtt
    14281 tgaatatcat caaatcccac gacacttaca tcatttggta tacttaatcc tctttcttta
    14341 agacctctta tgacacctat tgcaatcaaa tcatttgcac aaaatattgc atcaaaatct
    14401 atcttttcta aattttttat aaattcttgt ccccattcaa tcttatattt tccttcaaat
    14461 acataagcag gattgtagct taaattgcac tcgattagag cctttttata tccttctaat
    14521 ctatctactg ctatttcatt ttgaagtgga ccacttaggt atattatttt cttatttcca
    14581 atatctaata aatgttttgt agctatatag gccccttcaa agttatttat tttaacaatt
    14641 ccttttaagg tttctttgtt catacttatc ttcttatcaa tcaagacagt tggtattttt
    14701 atatctttat aatttgcttc tttccagttt gtatttgaag atgctgcaaa tataattcca
    14761 tctatcatcc tctctgatag tgttcttatt ccttcatatt cttttttagc atcttcatct
    14821 gtattacata aaattatatt gtagccttct tcattagctt tatcttctac acctcttgat
    14881 acatctgtaa agaatggatt tcgtatatct ggtataagaa gccctatagt attagttttt
    14941 ttagttacta aactttgtgc taatttattt ggtacataat ttttttcctt cattattttc
    15001 aaaatttttt ctcttgttgc ctgacttata ttttcatctt tattattaat gacttttgac
    15061 acagttgttt tagaaacacc agccaattcg ccaatttctt ttatagttat actcaagaag
    15121 ttcaccccca aaatttagga aaccgtttta cttaaccggt tacctagata ctatcacatc
    15181 tttttttcat ttgcaaccat tttatataaa ttttgatatt tttccctttt ttcaaaatat
    15241 tgcaacttat aattcaattt taatatttaa attaatctgc aaaaaaaaat taatatactt
    15301 ttataattat ctaaaagtat attaattaat ttaagcagta tttattaaag ttcaaatcct
    15361 tgctctataa tcattttttt atcattttca atcccacttc caacagttgt caaataatca
    15421 ccaacaattg cagaatttac tcccccatta aatccaactt tgtcatatcc ctttaatgat
    15481 agccttccac cagcatacct tatgtacaca ttaggaatta taaacctaaa tactgctgtt
    15541 gtttttagta tttccattgg ttctaataca tcataatttt ccattggtgt attcttaata
    15601 ggatttaaaa tgtttattgg aaatgatttt acatttaatt ctcgtatttc aaaagccatt
    15661 tttagtctct gttcttcact ctcattcatt ccaattatac cgcctacaca tacatctagc
    15721 ccagcttttt tagcatcttt tatagttttt atcctatctt tatatgtgtg tgttgtacat
    15781 atattatgaa aattatctct acacgtctca acattgtggt gatatgtagt cacacctgct
    15841 gacttcaaca ttttagcttg ctcataatct ataataccta aagatgcaca taattttaaa
    15901 ttagtatttt ccctcaatcc ttcatagata tttaaaatat tattaaactc ctttcctgac
    15961 atacctttac cacttgtaac caatgagaat ctatgcactc ctttactctc catctcttta
    16021 gctctattta agatttcatt gtaatctaat aatgaatatt cttctacacc agttttataa
    16081 taagaagact gagcacaaaa tttacagtct tcactacatt tgccagattt agcattcatt
    16141 atcgtacata aatctgcttt tcttcccata aaatattctc ttatttcatt tgcagatttt
    16201 aatagaatat caaaatcgtt tttattattt gtatcaaggc ttattaaatt aagagcttct
    16261 tcataactta tttctttttc tctcaatact ttatttttta attttattat gtatttttcc
    16321 atatatcctc ctatttgtca acttaatatt tttatgtagt ttacttatta tatatagtat
    16381 tgcatatata acatttattg gtcaacttta ttttatacac aagtttacta tacacaaaaa
    16441 aatactgctt caaaataata tt
//
LOCUS       DAKBNN010000078        16222 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000078 DAKBNN010000000
VERSION     DAKBNN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.078"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(346..867)
                     /locus_tag="OUB02_002102"
     CDS             complement(346..867)
                     /locus_tag="OUB02_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCT8885859.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIHHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            1033..1599
                     /locus_tag="OUB02_002103"
     CDS             1033..1599
                     /locus_tag="OUB02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCT8885860.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(1816..3879)
                     /locus_tag="OUB02_002104"
     CDS             complement(1816..3879)
                     /locus_tag="OUB02_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCT8885861.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(4260..7595)
                     /locus_tag="OUB02_002105"
     CDS             complement(4260..7595)
                     /locus_tag="OUB02_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCT8885862.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLYGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEYRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(7644..8072)
                     /locus_tag="OUB02_002106"
     CDS             complement(7644..8072)
                     /locus_tag="OUB02_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885863.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(8062..8442)
                     /locus_tag="OUB02_002107"
     CDS             complement(8062..8442)
                     /locus_tag="OUB02_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCT8885864.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(8475..8783)
                     /locus_tag="OUB02_002108"
     CDS             complement(8475..8783)
                     /locus_tag="OUB02_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885865.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(8816..10000)
                     /locus_tag="OUB02_002109"
     CDS             complement(8816..10000)
                     /locus_tag="OUB02_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCT8885866.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(10004..11467)
                     /locus_tag="OUB02_002110"
     CDS             complement(10004..11467)
                     /locus_tag="OUB02_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCT8885867.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFNDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIMGRVKALEKEIVDGLEELERMLEV"
     gene            complement(11977..12846)
                     /gene="gatY"
                     /locus_tag="OUB02_002111"
     CDS             complement(11977..12846)
                     /gene="gatY"
                     /locus_tag="OUB02_002111"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCT8885868.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(12866..14782)
                     /locus_tag="OUB02_002112"
     CDS             complement(12866..14782)
                     /locus_tag="OUB02_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCT8885869.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(14785..15705)
                     /locus_tag="OUB02_002113"
     CDS             complement(14785..15705)
                     /locus_tag="OUB02_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HCT8885870.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(15807..>16222)
                     /locus_tag="OUB02_002114"
     CDS             complement(15807..>16222)
                     /locus_tag="OUB02_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8885871.1"
                     /translation="SSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSK
                     SIKKDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKH
                     EHISISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
BASE COUNT         5071 a         2993 c         1602 g         6556 t
ORIGIN      
        1 aaaacgacaa caaataaata aaactaaaaa agatgttgtc tcaaaatata gataaattta
       61 tttttcattg ctaaaatgat taaaatatat ttgatttaat atacttgatt taatataaaa
      121 aaagagtgtc tcataaacta aaaagttcac ttttagacac tcctttatat atattatcta
      181 cttcttaatt catttattac ctctctacat tttctttcaa ctagtggata catctttata
      241 agtactaatt actccactta aaaatggttc ctgtactgtc catcagtcat atttttctgc
      301 caaatataat ggtgtttcat aatgagacaa taaatattta tatctctact tatcttctct
      361 aagctcgtca aatccaacta taggtatatt taaattatat tctttaagta gcttttctag
      421 ctcttctata aaaatacctt tcccatccac catttgtata tcatttattt tatccatcaa
      481 ttcattgcaa ccacttaact caccaaacca acgtttagaa ctacatgtct tattataacc
      541 acaacttgga ctgccatcta ttgcaatgat actttttata tcataatcat tttttaaata
      601 ttcatcaaac tgccttacat gatgtattaa catttgccta cattgttctc taaaaaacaa
      661 attgtcaaac tgttctttta catgtcccca cctatttatt ccatacataa tcatttctgg
      721 gcatggtagt tgaattattc catacccttt atttattaaa tctatcgcta tattttgtgc
      781 tcccttaaat tctgacaatc cctccacttt agaattacaa tttaatatac aatgacatac
      841 tactacaatc tctctatttc ttttcataat aatctcctta gttattctta attttaatga
      901 tactacatat tttgataatt atattattta tttgttctat atttccaata ttctatatag
      961 aaatatacaa ttagtaatct tttataatct atactatgat aaatgtatat atttttttag
     1021 gaggtattat aattggatac taaaaaagtc acattttctt caatgtgcat agttattaat
     1081 attgtcttag gtatggttat atctatgata aatataccat tattattttt agatacagtt
     1141 ggtactgtac ttggggctgt cgtattaggt cctttttggg gtgctcttat tggtggatgt
     1201 acaaacttag ttttaggtgt tatatcaggt cctacaaata taccttttgc ccttgtaaat
     1261 attgttcttg gtcttattgt tggatatatt tctaagaaaa aaggttttgg ttataaagaa
     1321 gctattatta caggagtaat tttatctatt gtttgtcctt taattggtac tcctatttca
     1381 gttttattgt ttggtggttt aagtggaagt ggtgctgatt tattggttgg ttttttagtt
     1441 cagtctggtc agaagatatt tactgctgcg ttctttccaa ggattcttag taatatagta
     1501 gacaaacctt tgtcttgtat tatggtggta tattttataa gtaaaatgcc taaaggtttt
     1561 ataaaacaat atagtaaaaa tacttctagt tcatattaat aaatcattta ttatgtaagg
     1621 tttattactc tttaaaatgc tatatactca tttttatata acatttttat taataattta
     1681 attagatagt atttgtatta ctttaattaa acgtaacctc ttgatataaa tttgagctat
     1741 aaatctatat caagaggtgc tattttattt ttaatataat taatattcta ttacgcaaaa
     1801 aatgcatata tttcattatt ttatggaaaa tattacttca tttaactcta tatcaaactc
     1861 attaagtaaa aatctaatta tatttaattt agcctctgta tttaaatttg tttctaaata
     1921 tatattttct gatattttaa taccttttct taatttatat gcatctctag acttaatatt
     1981 atctataaaa tctcttttat aagttttgtc tacaatactg ttaaacctta cagaatccaa
     2041 atcatataaa gtaagagcaa cttgttcata aaattctgtc caatttttag ccttatatga
     2101 ctctcctaat atactaaatg atactatact ttcatttgta aaattaacat catcatcctc
     2161 taacgtaaat atattttcac tgttacttgg aggttcatat gtaacacttg gatatttcca
     2221 aatttctaca gctctatcta gtaatatctt agttctattt tttatttctt cttcattcca
     2281 tttatctata gatgaaatat atttatttag ataaagccta ctatctttaa atccatcttc
     2341 catttctttc ttttcttcaa atgttttatt acttagtttt gaattatatc ctgtcaaagt
     2401 taaatttcca attgtatgta aatatttatc atgtatctcc ttatagttat ctcctagtga
     2461 ttccttccat ttatttgtta atgtttgtgg cattatatgc tctatattta gcatattatt
     2521 attgattaaa ttttctaaat caacaatttc tttattatta taattctcta acctctcaag
     2581 tatatataac ttattctttg acttagtatt atatatatct ttagtttcaa aagcatttga
     2641 aaattcactg tcagtaggaa atctttgaga tgatttctta tttataaaaa tatatcttaa
     2701 tatattagaa tagttttctt tataatcact aaatttttta atttcccttc ctaagttcat
     2761 aaatacttta ttcagtgcat tcggaggtac atcacatact attcttctta atataaaaca
     2821 ttctactaaa ttaagggatt ctacaagtac ttttttattt attatacctt cattataatc
     2881 atcaaataat tctaatataa acggatatga tacaaatatt tcaagtttgt ttatcctttt
     2941 caaaactttg tttacatcat tatcatcagt acatgaataa gatatctctc tataatagtt
     3001 agaaaattta agtaaatctt ttaataaaat ttctatatct ataccattat ctgatacata
     3061 ccttttaaaa ctaaaatata ctttattttt attcggtata cttctctcct ttatagtcaa
     3121 atagtctctt ataaaactac ttacgttgct acttgtattt ttttcaattt tataccaata
     3181 attattataa aattcctctt gtaacttaga gcttctaccc attaacacaa agtttcttac
     3241 tctatcagct tcacttaaat ctaagccagt tgaatttaga ctttcaaaga ttaattgagg
     3301 gtcatcttca ccatttttta actctatttc tactatagtt aatttctgta tagcatcaaa
     3361 caaatcatct attgatatat tatcttcttt tattctatta taaaaatata aataatttga
     3421 aactatatta gaatttctat ctgtattcat ttcttcttca aatagttcat agtacgcttt
     3481 tctatcttct gctattggtt ttaactttat ttttccatct ttatctcttg agtgcttgtt
     3541 tataagataa cactccttaa tctgttcttc tgttgtaata attccttttg attccccaaa
     3601 ctcttttatt atttgacaca tggcaatcaa taatattgac acagtagtca ctctttgttg
     3661 tccatctatt attaaatatt cttttcccat actatatata gatacaactg accctaagaa
     3721 atgagttcta aatccagaat ctattatatc ctctaaatca tcaaacaatc ttttacattg
     3781 ttctttcttc cagttataat ttctttgata tacaggtact acaaaacttt tgtttgaccc
     3841 ctctaaataa ctaagaaatt caatttctcc tgctttcata ttaactcccc ctataagtta
     3901 aataaattcc attcataact ccatgtatct atatattata tcaaaatttt gactcataaa
     3961 tgtatatgaa agccttatta tatacctttt tatacctgtt tttattttat taaagtaaat
     4021 acatgattat tctataaaat atacaaatac ttaattttaa aaattattat ttaatcttct
     4081 tcatgagtaa ttcatagtta tttatcaatt aaaaagagat attgcataat tattcaatac
     4141 ctctctttgt atatttacat tatccatatc tagttatcta tttcaaccca atattagact
     4201 acctactaat aaatttattc tctacatcat taacaataat tttgcactaa ataacatcat
     4261 tagcctatct ctgatgcatt ttctttaatt ttatttatag ttttaatcaa tttatttctc
     4321 tcttcaatat gattttcaaa taaatcaatt atacttccaa cagttctaaa tgggtcttcc
     4381 atcaacactt tgttatcctc tataaatcca ttcttgacta cataatcgac tataagttta
     4441 acaaaatgta tttgcttagt attaaagctc ttattattta aaaactctga aaaagcttca
     4501 tttgcagctt ctctatccaa tcccactagt cttctcacta attggcttac tggagtatct
     4561 ccaaactcct tcttataatc atcacaatta cctaactgtt ttaagagtat ttcctccaac
     4621 gtttcaacat cttgctttgt tatcttttta ttatttctta atttaaatat tgctaattca
     4681 tctttatggc tatttaaata atactctacc ttctttttat aattcttaag attgtttgca
     4741 ttatatacag aatcatttct atattctgct acaatcatat cttcaaaact tgtataatat
     4801 atcttttgac tagacttttc aagatactta ataagctctc ttaatgatat acgtacttct
     4861 tctacctcaa atatatctga atcttcccaa aaatcctctg tcattgcttt ttcaatagta
     4921 tatttttgtt gctgaatctg tggtatagtc ccaagttttg aaagtttttc tgctgtagcc
     4981 attacatgcc ttataccttt acttgcatta ttattttgta agtatgcaag ttgtatatta
     5041 tacataacta catcaaatct tttagccaat tcatcatcac tatatgaagt tatgagtggt
     5101 gatatatatt ctcttatctc attttgtgct actgctccaa tattgctcca aacattttca
     5161 tttttatact tatgtacata acttaaattc atcctaacta tataactatc ctcatttaaa
     5221 gcttttacag aagttataag ttcttctaac aattcttttc taagttctgc atactcacct
     5281 tctatatatt taatatcttg aagttcttta accatactta cctttatatt aaataatttt
     5341 tcagtaagtg tttctgcttt atttccctca aatcctttag gattaaattt aaaaaattca
     5401 aaattattac aaaaatcaaa tattaagaat ctctccttat ccatatcaac acctaataaa
     5461 tctggacata atctagttcc tctacctatc atttgccaaa acttagtctt acttctcact
     5521 ttcttgaaaa aaacaagatt tagtatttca ggtatgtcaa ttcctgtatc aagcatgtct
     5581 actgaaactg ctatttgagg taatttattc ttatcagaaa aatcatctat tatagaatct
     5641 acataattta tactatagtc aactgctttt acaaagttcc caccatactt tggatacagc
     5701 ttattaaatc tttcaactat tgccctagca tgctttgtat tctttgcaaa aattatagtc
     5761 ttaccaatct tttcttctcc ttctattttc aacccctctg tcattagctt atttaaaact
     5821 aaatctattg tgtcagaatt aaatagccac tcatttactg cattattccc tatttcgtct
     5881 ccaatactct catcatcatt aaatgtattt tcatattctt ccttctcttc atcagaaagc
     5941 tcatcatact taattccatc ttccataatc ttagtcttaa actcaatact agtataatct
     6001 actaaatatc catcctcgac tgccttatta tactcataag catatgtagg tactccattt
     6061 tccatgtcaa aaactgaata agtattttta tctatctcat cctttggtgt agcagtaaga
     6121 cccattaagt aagaatcaaa ataatcaaag attgacttat attttttata aatacttcta
     6181 tgcgattcat ctattataat taaatcaaaa tggccaggtg taaacaatct ctttccatcc
     6241 ttagacttcg tatcatcaat tgcattcatc attgttggat atgtagaaaa tatcatccta
     6301 gcatcttctg ggctgtcttt gctatccaat aaattacata acgccaagtt aggtaatagc
     6361 ttagtaaaac tttttttagc ttgctttacc aatgcttttc tatctgctag aaataatata
     6421 tttttaatcc aattatgtct tgaaagtaca tcaactatag atatagcaat tcttgttttt
     6481 cctgtaccag tagcacatac caaaagagtt ttgcgttgct tattttcaag agcctcacat
     6541 acagccctaa tagcttcttt ttgataatat ctattggata tttcatcatt tattgttaca
     6601 gaacttaaag acttcttact ctttcttcta tctatcataa gttgtaattc tgatttttta
     6661 taaaatccat aaacttttct atctgaatag tcatcccaaa tatacatatc aaaaccatta
     6721 gtcaaaaata ttactggtct ttgactatat ctcttttcca aacaatctgc atataattta
     6781 gcttgttgtt gaccaatttt tggatcttta ctcgttcttt tcgcctcaac tactgctaat
     6841 ggcttaccat tttcaccata aagaacataa tctacatatc ctacaccact ttcattaggc
     6901 ataccttgta cttctatttc ctcccctata tcttttccaa attcccaacc tgcaagcttt
     6961 aattctatat ttatatatct ttttcttgtc tcaaattcgc ttacttcgtc tatcttgaaa
     7021 tcatagttta cagtattaac ttttctcttt tcagttagtg actttcttaa ttcctcattt
     7081 tcttttatta cttcttcaag ctttttatct tttgaactta atctctcata aagattttta
     7141 agttcgtccg gtctcgtcct tttctcttcc ccataaagta ataaatcttc attaaagtct
     7201 tctgctgtat attcatctga ataacaatag tctatccaac taacaaattg atgcaaattg
     7261 ttaagtgaaa gtattgcttc tcctctctct atgttcgcac tagtatgtac agataaatta
     7321 cctaatttaa ctatatattt taataaaggt aacatatcat agtctataag ttctataaaa
     7381 ctattattat gaatcaacgt actgagatta tcttgataag gcaatactaa atcactatca
     7441 ttagcatata gccattttac tgcaagctct aaagctcttc ttgttatagt tgcacaagta
     7501 gatggactaa ctagtatact tttttctgct tctaagcaag cagtattaaa actattgaaa
     7561 tttttcttct tttgtaaaaa atcaaaatta cccaagcttt tatccccctt ttgtccctta
     7621 gttaaaataa atctctctaa aaactatttc ttacagctgt ttcttatact aacaaatact
     7681 tcttcatttt ctgtaaacaa ttcgtacata tcttcaaact gtggtatatc atatccatga
     7741 gaatcatcat ttctcaattt tctaagtgtt tctataacta tagaagaatt tttaggtaat
     7801 aaattgtgat actcacaagc taaaaggtag tcataaaagt ttttagattt tatgtttaaa
     7861 tcaattgatt tataatacaa cattgctttt gttaattcta gaaatgatac catatagctt
     7921 ttaaagcctt cttttatgta tctagcccat aactcaggca tttcttcctt atcatataag
     7981 attttgaagt tttgcaggtc atacaagcaa gaaccaaaat ccctatttac ttcttctatt
     8041 gagttccaag ataatttgtt tctaattacc atacatgtcc ctccaatcta agatatctat
     8101 tacatcactg ttttcaaaat agaatttatt tatataatcc ttaaaatctt tagattcata
     8161 aataacttca ccttctaaaa tagctttttt aagtgctatt gtataattat ctgtaacaat
     8221 ttttaggtct acatcaatgt ctaatatatt tcttagagct tctgtcttag ttgcaagttc
     8281 tgtaattggt acttttttga taggaatcca agctatgtct atatcacttt ctgatgtatg
     8341 tttatctgtt ccataactac caaatattat gattgctaga gtattttctt ttggaaatac
     8401 atttattatt gagtttttaa tctcatttgg tatatttctc atttaatcac ctcttgttta
     8461 ttaggtaata ttacttaatt aaatttatat ccataatctt ttaatctatt tatacatatt
     8521 tctctttctt catcagtaaa caactcttta tacttttctt ttaatactaa tacttctaat
     8581 gataaatcta atctcttaca ctcccatagt cttgtaaatc cttcagtagc tccttcttta
     8641 ttgattaatt ctttagcagc taataaggct cctttgttat ttatcagttg taaaaatctt
     8701 gttggcttat agttacattg taaatcagct tctttataga tatttaccat gttatggtat
     8761 aaatctagct ctatttgatt catagatttt attcccccat tttaataatt gtattttagt
     8821 tgaataactc tcctttaaaa gctttttgca ttaatgagtt aaagttgtct tctagttctt
     8881 ttaagctagt ttccatttca aacttcaaag agtttacttg tttaacgaag ttggcaaatt
     8941 gattttgaag tctaatagga ggtacaataa tatttatttt tctaacagca tctttagtta
     9001 tcgtttttgt tacagctcct cttactttct cttttatttt actttccata taggatgtta
     9061 gtaaaaggtt atataagaat atatgattca ctttatcttc attaaatcta agcataaatt
     9121 gattcatagc taaagatact ggtttattta aaaatggcat taaataaact ttacctgcac
     9181 tacctatctt atttattata acctcaccac caaacacctt tgatttttca agataattat
     9241 atgcatgttc atcgatatac ttaacacctt tttcaaagtt attattttct aaatctgtag
     9301 ttcttaccat taaggcataa ccttttgtat ctaaaagagt tacattatct cttaaggttt
     9361 cataactacc atttgaatga taatcagtta aaacatctaa atatttccct atttctgaaa
     9421 tttcccagtt tttttcattc tttgatggag taccaaacat ctcaataaat cttgatttaa
     9481 ctaattcatc caatacttct atttgctctt ttcttttatc tattaactct tgagccttgt
     9541 ctaaaacacg cactaccttt tcttgcactt ttaaattata catgcgtatt tttatattag
     9601 ctaatccttc attatttaaa gctgattgtg tagcaccttt acttaaccta tttttttgat
     9661 ttagaaaata actactattt aagtaatggt ataaatattg aggcaatatt tcttttgatg
     9721 gttttaaaac ataaaatcca gtcgatacta ttatattttt attagtttta ttaataacta
     9781 atgttttctt tgtgtccttc atttttgcaa atataacttc tcctatattt acagtttgat
     9841 ttgctctaga aggtttattt tcataagtaa ctatttctag attgcttatc ttgttaaaat
     9901 ctaagtttcc agtactaaga tatgagcgtt ctccatcaaa cttttttaca ttttccccgc
     9961 ttatactaac aacatctaaa atttttgctt catcaaccat actctacacc tctaacattc
    10021 tttctagttc ctctaatcca tccactatct ctttctctaa cgccttaacc ctacccataa
    10081 taacactagg agcatcataa actacttctt catacactac ctctttatac ttattaatag
    10141 ataaatcata cccattctct cttatctctt ccacatcaac aaaaaaactt tgctctgttc
    10201 tttttctatc tacttcacca tcaagattat caaatctctt aataatatcc tctatatcac
    10261 tatcctttat ctcatttctc ttatcatcca aagaataacc atcagctttc atatcataga
    10321 accatacctt atcagttcca cctgttcctg ttttagtaaa tatcattata gctgtagata
    10381 ctcctgcata aggcttaaac actccacttg gcattgatat tatagcttcc aatttatgat
    10441 tatcaactat ttcttttctt atcgatttat gtccacctgt tgaaccaaac aatactccat
    10501 ctggcactat acatgcgcat cttccaccaa tttttaaaac tcttaaaaaa agtgccaaga
    10561 ataataactc tgttttcttt gtcttagtca cttttaataa atctgctgac actgcttcat
    10621 aatctaaact tcccttaaaa ggaggatttg ctaacaccaa tgtaaactta tctttatctg
    10681 tattaacctc cgataaagaa tccttatact ctatattagg attgtcaacg ccatgcaaca
    10741 tcatattcat agcacctatt ctaagcatag ttctatccat atcaaatcca tagaacatac
    10801 cattattaaa atgctccttc agcccttgca ctaagaaaag acttgaatga ttatccctta
    10861 agtattgttg tgatgatact aaaaaacctg ctgaacctgc tgccgggtct actataatat
    10921 cttctggtgt tggtttaata agtcttacta tcatatctat tatgtgtctt ggagtcctaa
    10981 attgaccatt agtacctgct gtagccactt tggataataa atactcatac aaatctccct
    11041 ttgtatctct atccttactt aattctaaat tactaatacc atcaactatt ttagataaca
    11101 taagaggcgt aggaatttta aatattgcat ctcccatgta ctttgcataa gctgagtctt
    11161 tatttccatg taattttttt atgaaaggaa acacttcatt ttgtactatt tcatacattt
    11221 tttctgaatt tccttcatta gaaaacttac tccatcttaa atgttgatgt tcatcattaa
    11281 atatcctttc aaaagaaact cccaaaagag ttgcttctgc ttcatttttt gtttctactt
    11341 catccaatcc tttaataaat aaaagatatg taaattgttc tataacttct aatggattag
    11401 ttaatccacc agtccaaaat gtctcccaaa tcttgtctac tttgctcctt aattctcctg
    11461 taatcatata tgactttccc tccagtgttc ataaaataat ttaaatatat attttagtcg
    11521 tacgccttaa tagttagtgt cttatgttaa atccaagttt ttactacaca gttattaaat
    11581 tatttttcct atttacatgt tttagttaaa aaactaatgc aatactctta ttttatgatt
    11641 attatatata ccatacaaaa ttattttatc atatatccat catagaagtc atctacctgg
    11701 ataattctta ccatattata actttttaat attaatttaa acttagaaag aactttttta
    11761 tttaattata tcatcttcct tatttcaaat ttctcaatta caatattaat caaaaaataa
    11821 aataagatgc tgtctcaaaa tagatataat cttctttttt attgtcagaa tgatgaaaat
    11881 atatttaatt tcatacaaaa aagagtgcct catgaactaa aaagttcatt ttgagacacc
    11941 ccttaattta atcttactta ttctttccct ttattattaa cacttcactg tctctattgc
    12001 ttttcctaca catccacaaa cttccatcct actcttaaca aaatctttaa ctgactctaa
    12061 acctttttta ccatatttct tagggtcaat tacctctggg tcagaattaa gcacattctt
    12121 aactccattg ctataagcaa tccttaattc agttgcataa ttaaccttgc aaatccctct
    12181 ttttatagat tccttaataa cttcatctgg tattccagac ccaccatgta atactaatgg
    12241 aactgataca acatctctaa tttcagataa tctatctaaa tcaagctttg gctctccctt
    12301 atataatcca tgtgccgtac ctattgctac tgctaaagat gatacccctg ttctctctac
    12361 aaactctttt gcttctaatg ggtctgtata tgctccccca tcaccaccat ctaaatcgtc
    12421 ttccttacct ccaactttac ctaattcagc ttcaacaggt atatctgaag gcatacatgc
    12481 atctacaaca gatttagata ttgaaatatt atcttcataa ctttcatgag aaccatcaat
    12541 cataatagaa gtatatccaa ctctaagtgc ctgcatagct agtccaaagc ttgaaccatg
    12601 gtctaagtgc attgccacag gaacacttgc cttctctgat gctaccttta cattcgctaa
    12661 ataataatct agtcctgcat actttattgt tgaaggtgtt gtttgcaata ttactggtga
    12721 tttaagttct tcagcagcct ctattactgc cataaccatc tccatatttt caacattaaa
    12781 tgcacctact gcataatgcc cttcttgtgc atctaataat atttgtttag ttgtcactaa
    12841 tgccataatt tctcctccta tatgtttata ctgatttaat ttcttcgtta tttaattttg
    12901 atactatttc tctacttgtt ttgctattta ataactcatt taaaaattct tcattcatta
    12961 attttgaaga tagttgagat aatgttttta agtgtaaatt atcaccatta tctgtagctg
    13021 ctatcataaa tataatatgt gccaagcttc catcttctga ttcaaagtca aacccttctt
    13081 ttgatacacc tactgctaca cgtggtacct ttactgcatc tgtttttgca tgtggtatcg
    13141 ctatccccat accaaaacct gtagatgata tttcttctct tttaaggatt tcttctttaa
    13201 acttcttttt atctaaaaga acaccagatt tatcaagtat tccaatcatt tcatcaatta
    13261 catctgtttt attttttccc ttaacatcta aaattactgt ttcttcgcta tataaagaga
    13321 tattattaga tacgttttcc gattctgcac tttcattttt atgttcatct tttgttgcat
    13381 ttgtattttc tatagtattt ttctcattta ctactttagg tgtaaccatc atataaagta
    13441 cagcacctac taatgaacct acaaatatac accctaccca taaaagtggc ttattgacta
    13501 atcctagaac caaaaaccct ccatgtggag ctggtacttc tattttcatt atataagtta
    13561 aaattgatga tattgatgat gcaatcataa gtactggcaa cacttttaat ggattttttg
    13621 ctgcaaaagg tattgccccc tctgttatat gagtactacc tagtatataa ttgattaatc
    13681 ctgctgctct atcttcttct gtaaatctat ttttaaataa agtagatgct attgctatta
    13741 ccaatggtgg tgttatacaa gctgcagata cacctgccat aaataaataa tttccctgtc
    13801 ctagaagcat agttccagtt acatatgctg ctttatttac tggacctccc atatcaaatg
    13861 ctgacataca tcctattact attcccaaaa ttatagggct tgaattttgt aaccccgata
    13921 gaaagttcat catagcattg ttaagtgctg aacatggccc accaagtaac aacattgcta
    13981 cacccacaag acctacgctg acaataggaa ttataaaaat cgatttaaga ccttctaatt
    14041 gtcgtggtaa ttttcttaac aaatgtacaa atagtttaac taagtaacct gctaaaaatc
    14101 ctgatatgat tccacctaaa aaccccgaac ctgtatcaaa tgatatcatt cctccaacaa
    14161 gtcctgccaa cataccagct cttcccgata tagattgagc tataaatgct gccaatacag
    14221 gaaccatcaa gttcatagaa tatccaccaa cagattttaa tgtagctgct atttgattat
    14281 attgttctga atttgggtca aatgaataaa tcccccataa aaatgatata gcagtaagta
    14341 ctccacctgc tactactaaa ggtaacatgt gagatacacc attcattaag tctttataca
    14401 acttttgacc aaacgataag tttccttctt ctgcaatttt actgcttgat aagtttcctt
    14461 ttctaatagc tacttttcca tcaatacatc tttgtataag ttcattagct ttatgtatac
    14521 cttctttgac aggtacttct attactggct ttccattgaa tctgtccata tcaacatttt
    14581 tatcacaagc aactattatt gcatctgcca tttcgatatc ttttgctgtt aacttatttt
    14641 ctacacctat agctccattc gtttcaactt ttatgtcaca ccctaattcc ctggctgaat
    14701 tttttaatgc ttcttctgcc ataaaagtat gagctattcc agttggacaa cctgttaccc
    14761 ctagaatcat tttctttccc atttttatac cacctttctg actcttatat tttttatata
    14821 atcttccaca ttctccaaat tacctatacc attactttca gccacacttg ctcctgttgc
    14881 tgctgacctt tttaatgctt cttcaatatg ttctggattt tccaaccata tacttaaaaa
    14941 agcagctaat gcagaatcac cagcacatgc agagctgact actgtaactg gttgagcact
    15001 agcataataa attgattttc cattataaaa ataagaacct ttttctccta aagttagcaa
    15061 tatattttga gcacctttta aatgtagtaa tttaagtaca tccttaatat cttcttcatc
    15121 tcttacaata attccaaata tatctttgat ttcttcatca tttggtttta tcaaatatgg
    15181 attatactgt agtaactcct ttaattttgg tgaacttata tcgagtattg tctttatact
    15241 tttattttta caaattttaa atatatcctc atagtaatta tcatcaatac caggaggtag
    15301 acttccactt attgataagt aacttatatc tgctaatgac tgtatcttat ctagcatatc
    15361 tagtttttgt ttttcattta catatgaccc agagtttaca aatttaaatt catcacttcc
    15421 atcatttaaa aatatattta tacgagttgt atcttctacc caagtaggca atacgtcaaa
    15481 tcctctcttt tcgctttctt caactatata ttttcctgaa aatcctccaa aaaatcctaa
    15541 tatcttagat tctataccat aatgacctag tacaaaagtt acatttaatc cctttccatt
    15601 aggagaatat acagcatttg atgtacgatt tactaatttt ctctttatcc cattagtaga
    15661 tatattcata tctattgcag gatttgtagt taatgtatat atcattgtct tcccccttgc
    15721 ttgtgctgtg tatgttttca ttataataaa aaagtctatg aaaatctttt cacaaataga
    15781 aaatcgattt tcatagactt ttcaatttaa ttttcagaat tattatatct gaccatataa
    15841 tctataatta tcctagatat aatatacaag ggcaatctgg aagatacttc atattcagaa
    15901 attgatatat gttcatgttt gtatcctaca atatataaat ccgaatactt tataagtttt
    15961 gcactttcac tacaacagca agttataacc ttagctccaa ctaaattggc attctgtgct
    16021 gattctacaa gctctatggt atttccattc aatgaaaata taattataag ttcatctttt
    16081 tttattgatt tagattttat tttcattatg ttggggtcat ttataaaaac aacattaaaa
    16141 tcaagtaatt gcaatttaag actaaactct tttgctacat attcagtaag ccctcttgct
    16201 aaaatatata tcttactaga tt
//
LOCUS       DAKBNN010000079        16217 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000079 DAKBNN010000000
VERSION     DAKBNN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.079"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(115..849)
                     /locus_tag="OUB02_002115"
     CDS             complement(115..849)
                     /locus_tag="OUB02_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCT8885872.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            974..1690
                     /locus_tag="OUB02_002116"
     CDS             974..1690
                     /locus_tag="OUB02_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCT8885873.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDNDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1824..2516
                     /locus_tag="OUB02_002117"
     CDS             1824..2516
                     /locus_tag="OUB02_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885874.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2506..3687
                     /locus_tag="OUB02_002118"
     CDS             2506..3687
                     /locus_tag="OUB02_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCT8885875.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTMFEVQLPIIQS
                     A"
     gene            3969..4424
                     /locus_tag="OUB02_002119"
     CDS             3969..4424
                     /locus_tag="OUB02_002119"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCT8885876.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4414..5139
                     /locus_tag="OUB02_002120"
     CDS             4414..5139
                     /locus_tag="OUB02_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HCT8885877.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5111..7492
                     /locus_tag="OUB02_002121"
     CDS             5111..7492
                     /locus_tag="OUB02_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HCT8885878.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKNRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7697..8926)
                     /locus_tag="OUB02_002122"
     CDS             complement(7697..8926)
                     /locus_tag="OUB02_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCT8885879.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9527..10441
                     /locus_tag="OUB02_002123"
     CDS             9527..10441
                     /locus_tag="OUB02_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885880.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10465..11100
                     /locus_tag="OUB02_002124"
     CDS             10465..11100
                     /locus_tag="OUB02_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HCT8885881.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11476..12192
                     /gene="pyrF"
                     /locus_tag="OUB02_002125"
     CDS             11476..12192
                     /gene="pyrF"
                     /locus_tag="OUB02_002125"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCT8885882.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12218..13261
                     /gene="carA"
                     /locus_tag="OUB02_002126"
     CDS             12218..13261
                     /gene="carA"
                     /locus_tag="OUB02_002126"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCT8885883.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGIKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13314..>16217
                     /gene="carB"
                     /locus_tag="OUB02_002127"
     CDS             13314..>16217
                     /gene="carB"
                     /locus_tag="OUB02_002127"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCT8885884.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTF"
BASE COUNT         6535 a         1788 c         2931 g         4963 t
ORIGIN      
        1 aatatagatt aaagataata acatataata aataaaaaag taagattgtg tagattatat
       61 aaagattatt tacaattaat acaatcttgc ttttttattt aaaaatttta attattattt
      121 aaaatttttt atcttctctt tatcccatat aaacaatttc tcagaattat ttaaagcacc
      181 aaatacattt cttttaaaat caggcatctc tttatttaaa ttagcaataa gattttttat
      241 gtactctctc ctagtatgac catttgcagg gcagggattt gtgataatag gatagttaga
      301 atcttttgaa attttcttag tcatatactc ttctatgtaa accataggtc ttataactgt
      361 taaatcctgt ctgtctaggt atgtctttgg ggaaaaacaa tttactctac cctcataaaa
      421 catagacatt aaaaatgtct caattgcatc atctttatga tgaccaagag ctactttatt
      481 acatccaagt tttttagcat tatcgttcaa agcacctctt cttaaattag cacataatgc
      541 acaaggattc ttttcttgtc ttatatcaaa tacaatttct tttatgtcag tttgaaattc
      601 ataaaaagga acatttattt ctttacacaa tttatctagt ggagaattat caacacctcc
      661 agtatttaat gttatggcta tcaattcaaa gttttgtgga gaaaatcttt gataactttt
      721 aagtatatga agtaaagtta aactatcttt tccaccagaa agtccaaccg caattttatc
      781 attttcttgt atcatatcaa aatcttgtat agccttacga gctttactta gaagtttttg
      841 tatagacatt ttatcaccat cctttaaaaa tattaaattt aagatttatt taagatttac
      901 ttataaaata cttaagtctt atactctaaa attaacatta tagcaaacat aacaaaaagg
      961 agaagattta attatgaaaa aatttaaatt aataggagcc attattttag tagtatttat
     1021 aagtgtattt atatctaaag atagattact taagaaagat aacgatccaa tagtagctgc
     1081 tcaacatgat tcagaacaag atgttgaggc aaattctcaa aataaaaatc actcaaatag
     1141 tatatcaaat atattattgg taaacaagac aaatggaatt agcaaaaact atactcctga
     1201 aaatattact aaggtaaaca taccttttgt tgaagaagca acagaggaag aaaaacaaat
     1261 ggcaggagaa cctgcaaagg cagttgaaga tttagttaag caagctaatt cagaaggaat
     1321 acagttttta gggtcttcag catacagatc ttatgatact caactggata cttatacaag
     1381 aagggtaaaa tctcagggac gagaaaaggc agatgcatat gtagcaaagc ctggttacag
     1441 tgagcaccaa actggacttt gtattgattt aacaaatcca gaaagatggt ttgttggaag
     1501 tacaaaagaa gctaaatggt tggcagaaaa tgctcacaag tttggattta taataagata
     1561 tcctgaaggt aaagaagata taacaggtac agcatatgaa ccatggcata ttagatatgt
     1621 tggtaaggat gctgccgagg aaatttattc aaaaggatta actttagaag aatatttaca
     1681 aaataaataa tataaatgtg taaataattt gtattatatt ggaaatttag aaattaataa
     1741 atttatagat tacactaact aaaatatagt ataattttat atgaataaag ctatatacaa
     1801 agattaagtt aggatgtgta aaaatgaata atacagttct tgttgtggat gatgaaaagg
     1861 aaataagaga ccttatagag atatatcttt taaatagtgg atataatgta attaaagctg
     1921 aagatggaaa agaggctcta gatattttaa aaaaggaaaa tattcattta atgatattgg
     1981 atataatgat gcctaaaatg gatgggatag aagtttgtag aaaagttaga gaaagtttga
     2041 atataccaat acttatgtta agtgcaaaat cagaagacat ggataaaata caagggataa
     2101 tgactggtgc agatgactat ttgacaaaac catttaaccc acttgaactt actgtgagag
     2161 tgaagtcgct gattagaaga gcatattatt ttagtggtgc taataatgaa gataaagatg
     2221 tcataaaggt tggagtagta attatagata aggaaaaaca tagtgtaaca gtggctggaa
     2281 atgaaataat tttgacatca agagaatttg atatattata tttactagca aataataaag
     2341 gcagagtata tagtacagaa gaaatatttg aaaaggtatg gaaagagaaa tattaccaat
     2401 ctaataatac tgtaatggtt catatgagca gaataagaga taaaattgaa aaatatacag
     2461 atggagaaaa aataattcat actgtatggg gagtaggata taaaattgaa aaataaaagt
     2521 ttattaaatc atatgtttcg acccatatta gatcttataa taagctgtgc tttaacttca
     2581 atgcttattt taacaagtat gtatataata aaatcattat atgtcaatag tgatatgtct
     2641 gtagttaatc aacttgtata tcaagcaaga gatttcagaa gtcttatagg gtatgattta
     2701 ttttatccat ttttatttac aatcacatca ttagcaattt attccattat atcgtacaag
     2761 agaaataagc aaattgtaga ttttataaga aagactaata gaaaaaatca aaaattagaa
     2821 atcattaaaa gtgctgtaaa tttaatggat gaaggtaact tagaaaaaag tatagatatt
     2881 gagtatgatt tagatatatt aaaggataaa gaaaaggatg atattgatga actagcacat
     2941 aaaatcaata atatagtcca tcaacttaga aatataacta ttgaagagag acaagctcag
     3001 cagacaaaaa cagatttaat aacaaatgtg tcacatgact taagaacacc tttgacatcc
     3061 ataatgggtt atttaggagt gatagaagaa ggtaagtata aagatgaagt tcagatgatg
     3121 tattatgttg atattgcata tgaaaaatcg aaaaatttaa atgttcttat aaatgatttg
     3181 tttgaattga ctaaaatgca aaataataca ataaaattaa acaaaataga aataaattta
     3241 gtagagttat taagtcaagt agtatctcaa tttgaaattt attttaaaca agaatttatg
     3301 gttagtagaa ttaattttaa tgaagaaaaa ttaattgtaa aagctgatcc aaataaattg
     3361 gtaagagcat ttgaaaatct tataactaat gctgtgaaat atggaaaaga tgggcattat
     3421 gtagatatag tcacagagaa aaaggaagat atggcggttg taaaagtgat aaattatggg
     3481 gaaccaattc cagtgttaga tttaccaaat atatttgata gattttatag agttgaaaaa
     3541 tcgagaaata gaaatgttgg aggttctgga ttgggtcttg cgattacaaa aaatattgta
     3601 aatttacatg atggagaaat tactgtttca agcgataaat atcaaactat gtttgaagta
     3661 caactgccaa taatacaatc agcttagtta taaaataaaa gtacgattat gtgatataaa
     3721 tattataaca ataaagtgtt tacaaaaata aaaaagtaat atataattta agtcagttta
     3781 ataagatact tatcaagagt taggggagag agttagctca gtgatcctat accaaccttt
     3841 tatcaggtgg tcctgctaac aacgatgaga tgacttgaaa taatatactc ttcttatcaa
     3901 taatagatag gaagagtttt ttttagaatt aatgtaacaa attttaaagt ataatggagg
     3961 attaaataat ggaaaaagta gaaagtttta agctagacca tacaaaagta aaagctcctt
     4021 ttgtaagaaa gtgtagtgta cttgatggag taaagggaga taaagtaact aaatttgact
     4081 tgagattttt acagccaaat gtagaaagtt ttggaactgc tgctatgcac ggtttagagc
     4141 atcttttagc tacttattta agagatacat tagatggtgt aatagactta tctccaatgg
     4201 gatgtagaac aggattttat ttaatactat ggggagatgt agatgccaaa acagttaaga
     4261 ttggtttaga agaagcatta aaaaaagttc ttgaatcaga taaaatgcca gcagctacag
     4321 ctatagaatg tggaaactac agagacttat ctttatttgg ggcaaaggaa tatgcaaaag
     4381 atgttttaga taaaggattt tctttaaata tatatggaga ataatttaat tggaagagaa
     4441 gatataagca taaatcaaag tgaagtttta agatatttac aatacagagg tcaagagata
     4501 gataaagacc ttaaatatac tattgatgaa tgtgtagcta taactaaaaa taaaataaat
     4561 cctagatatt tatctaggat ttatcctatt aaaataaata aagataatgt tgtagaatta
     4621 gagggaacaa atttaacatt agagagtaaa gatgtatatg aacttttaaa agaatgtaat
     4681 gagtgtgtaa ttatggcagc tactataggg atagatatag aaaaagaaat aagacgacac
     4741 tcttattcaa atttaacaaa aggactaata attgattcat gtggaacaac agcaatagaa
     4801 gaagtgtgtg acattgttca aaataattta gaaaaggttt ttttaaagca agataaattt
     4861 ataactatgc gatatagccc aggatatgga gatttaccaa tagaaaaaaa tatagatata
     4921 ttaaacgttt tgaatgctca aaaacaaata ggtctaacaa ttacttctaa tggaattatg
     4981 ataccgagaa agtcagtagt ggctattata ggtatttcta atacaagaat aaataaatct
     5041 aagaaagaaa agtcgtgtga aaattgtaaa aactatagaa actgtacata tagaaaagga
     5101 gcgaactctt gtggaaataa gacaatatct gaaaaataat atattaatat ttgatggtgc
     5161 aatgggaaca atgcttcaac aaaaagggtt aaagttaggt gaaaatccag aagtatttgg
     5221 tcttcaaaat ccagataaac ttatagaaat acatacagca tacttagagg caggttcgaa
     5281 tgtaattctt acaaatactt ttggatgtaa tgaactaaaa ttagatagta agtatactgt
     5341 agaagaagtg attgacaatg ctgttttggt ggctagaaaa gctatagaaa atgtagataa
     5401 tactaaacca agatatgtag ctttagatat tggtcctata ggagaaatgc tagagcctat
     5461 gggtacttta agttttgata aagcatatga aatatttaaa agacaagtac tacaaggcgt
     5521 taaatctgga gttgatgtaa tagtaataga gactatgatg gatttatatg aagctaaagt
     5581 agctgtatta gcagcaaaag aaaactctga tttacctatt ttctgtacta tgacatttga
     5641 tgaaggtgga agaagtttta ctggatgtat gccagaatgt atggtggcta ctatagaagg
     5701 tctaggtgta gatgcaatag gagttaattg ttcattagga cctaaacaac ttttaccaat
     5761 agttgaaaaa atagcatcaa gagctacagt ccctgttatg gttcaagcaa atgcaggtct
     5821 tccaaatata gttgatggag aggctatata tgatgttgat gcaaaagaat tttttgaagg
     5881 ggttaaaaag ttcgttgaag ttggtgctac tataataggt ggatgttgtg gtacgaatcc
     5941 aagttttata aaagaaatat ctgagaatat aaattctgtt acaaagggat gtatcgagaa
     6001 aattgataaa tgtgtagtct gttcaccatc aaaatttgtg gaagtacaat cgcctacagt
     6061 tgttggagaa agattgaatc ccacaggtag aaaatctcta caagaagcat taaaaaatga
     6121 aaatgttgat tatgctataa atttaggttt agaacaagta aatgctggag cacaaatact
     6181 tggagtaaat gttggtcttc cagagattga tgaaaaaaaa ctaatgccaa aattaataag
     6241 agagattcaa gcagtagtag atacaccttt acaagttgat tcttccaatg tagaggcctt
     6301 agagcaagga cttagatatt ataatggtag aacaatagta aactctgtaa atggcaaaga
     6361 ggagtcttta gaaagtatat taccaatagt taaaaagtat ggttcatgtg tggttggact
     6421 tacacttgat gaaaagggga tacctaaaaa ggctgaagaa agatttgaaa tagcaaaaag
     6481 gatagttaat agagcagttt cttatgggat aaaaccaaaa gatatattta tagactgttt
     6541 atcattaact gtttcagcac aacaagaaga agcaatagaa actataaaag ctataacaat
     6601 ggtaaagaca ctaggagtaa aaactatatt aggtgtttca aatatttcat ttggtcttcc
     6661 aaataggaaa gcacttaatg cttcttttct aacattagct ttaggggcag ggcttgattt
     6721 agcaattata aatccaaatg aatatagtat gatggaagct atcaattctt ttaaaatatt
     6781 aaataacaca gataaaggat gtataaacta tataaatcaa tatagcaata taaataattc
     6841 aaaaaagtcg gattcatcta caaagattga taaagatttg cccttggata ttttagttga
     6901 aagaggatta aaagatgaag caaaaaatgt aacactgaat cttttaaaag agaaggatga
     6961 aaattatata ttagatgaga ttttaattcc tgctcttgat aaagtaggaa aaagatatga
     7021 tagtggagat atatttttac cacaattgat tcaatcagca gaaacggtaa aagtttcatt
     7081 aaatacaata aaggaaactt tattaagtaa aagtagcaat aatgtatcca aaggaaaaat
     7141 aatagttgct acagtaaaag gtgatatcca tgatattggg aaaaatatag taaaaataat
     7201 gctagaaaat tatggatatg aggttataga tttaggtaag gatgtcccta ttgaagaagt
     7261 agtggaagtt gctaaaaata gaaatataaa attagtagga ttaagtgcac ttatgactac
     7321 gactgttcaa agtatgaaag atacaattca ggcactgaga gataatgaga taaatgcaaa
     7381 ggtatttgta ggaggtgcag ttttgacaga agaatacgct gaagaaatgg gtgctgatta
     7441 ttattcaaag gatgcaaagt ctgctgttga aatagcaaag ttaaattttt agcatataaa
     7501 ttaaaataaa ggttttacta aataggatat tttatatata atataaatat aagttttaga
     7561 tacttatgtt taaaacaata ggaattaatc tataataatg agattaatta atataaaaaa
     7621 tatttatcat caacattaaa aagggctggg aagtctggta taacttccat cagccctttt
     7681 tagttaaaat catatgttaa aaagcccaat taccattttt aaaaatctgt atttgctttc
     7741 catcataagt ctcaccaata atatccatat cagcagaacc aatcataaaa tctacatgtg
     7801 ttaaactatc atttacacca tgttcatcca attcttcatc ttttaaattg tttccacctt
     7861 ccagacaagt cttataagca gaaccaattg caaagtggca agaagcattt tcatcatata
     7921 gagtattata aaaaattata tccgtattgg atattggcga gttgtatgga acaagagcaa
     7981 cttctcctaa ataatgagag ccttcatccg tttcaataag tttttctagt gtatctagtc
     8041 ccttttcagc agagaaatct attattttac catctttaaa tgttaagaag aaattgtcaa
     8101 ttatgtttcc accataaaca agaggttttg tactataaac agttccattt actttaaact
     8161 tatgtggcat tccaaaaatc tcttcagttg gaatattcgg attaaaactt attccattag
     8221 ggtctttgga tgctccactt agccaaacat gaccctttgg caagtctaga gtcaaatcag
     8281 tttttgagga tttatatttt aaagttttaa agttattttt atttagaaag tccattttag
     8341 atttaagatt ttcattatgt tcttcccatg cttttatagg gtcctgacca tctactcttg
     8401 aacatttaaa tatagcatcc cacagttttt ctattgcttt atcttctgat aaatttggaa
     8461 acactttttt tgcccaatct tttgttggca cagagactat actccattta catttatcag
     8521 ttagagtgta agaacgccat tcttttaaag cttttccaga tgccttctga aaatttgcaa
     8581 ctctttcagg gtcaacatct tttaataaat ctggattttg ggcatagact gataaaaatg
     8641 ccccaccttc ttttgctata tctacatatt gttctgctgt ccatttgggg aactcatgaa
     8701 atgcttcatc tggagcatat aagtatttaa taagagtact ttcttcatca gaccactcta
     8761 tataaacatt tttagcacca catttatatg cttctttaag tgcatttctt gcaaaatcag
     8821 cacattctat tggtgacttt ataagtagaa tttgacctgg ctgtatgtta actccaactt
     8881 ttattgctaa ttcagcgtat ttttctaaat ttctatttaa attcatattt atcctcctat
     8941 atttttaata ttgaataact tataaagtat tataacatta cttatgtttt taatattgaa
     9001 caacttataa aatattataa ctttacttag attttgggtc atattcaaaa tatttatact
     9061 ttatagattg agaaataaaa aaaatatttt aattaaaaca aattatgagg ataacaaact
     9121 tatttaagca gtatgttttt tgttgataat aatattaaag tagaaataaa aatacttttt
     9181 tagtgaaaag ctatacaaga ataccgaaaa aaacttataa tataataatg agatagatat
     9241 ttataaatga aaaaaaattt ttctggcaaa gcttaattaa aaaggtaata gctagtgttg
     9301 ttatttaaat tagcatagaa aatcaaataa aaaaattatg ctaatgtata gacattgata
     9361 aggtagaatt taagcctttt gaatgctata ttaaactaga gtagatattc ttatgaattt
     9421 caaaataggg caggaggaaa aaggaaataa aactataaaa aatataaagt atttatataa
     9481 atttatagag tttatacgtc agaagatttc agttaggagg gcataaatgg agctttatga
     9541 agccattttt tatagaaaat ctgtaagaaa tttttccagt aagaagataa aaaagccatt
     9601 gatggaagaa ataaaaagaa gttgtaaaaa tttagaatac ttaaatgaag atttaaatat
     9661 taaagcacat gttgttgata gaggtcatat aatacatttt ttgatgggta aggaatgtaa
     9721 agtaaaggca cctcattata tagtagttac atcagacaaa ggtgaggact atttacagaa
     9781 tataggattt gctacagaag gtgtagtttt gcgccttaca acattgggaa tagctacttg
     9841 ctggttggaa ggaaacttaa agagagaaga tatcttggaa tttgtagata ttgggaataa
     9901 gaattttgaa gaagatgagg aagatgattt agattatcta gctggaataa aaacagaaga
     9961 agatgatgaa aacaaaatag aaaatcctta tataataata gcatttggat atccagcaaa
    10021 aaatgaagag ttgtttagga cgagatatgc tagacctgat agaaagccag taaaagatat
    10081 gataaataag ataaatagga atagaaagtg gaaagatatc ttagaattga caagaagagc
    10141 tccatctgta aaaaattctc aaccgtggta tttccataag gatgaacgag gtcttcattt
    10201 atttgaaaag agacctaaga aacactgtga agatatgaat aaggttagct taggtgtagc
    10261 cttaagacat tttgatatag cttgtataaa aaataaaata gatgtttcat atgaaaaact
    10321 tcctataagg aataaaatag gaaaatcata ttttataact gtagtcgagc atgtgaaacc
    10381 agaagaagaa acccaagaag aaaatgtaac tctagaaaaa gaagaatcgc aagatgaata
    10441 aaattttgga aggggatatt ggatatgtat aagtgtggaa aatgtaatct tagaggctgt
    10501 tctaatggaa atatggataa attaatgaag atatgtccaa ctagagaaat tgaactacaa
    10561 caaaaagcaa agcaacttta tgaagaagaa gagaatagaa aaatagctta taatgctgga
    10621 ttagttgaag cagaggggta ttgtcaatat actagactaa aagagattat agagtttgct
    10681 aaaaagtgtg gttataaaaa gattggcata gcattttgta ttggactaaa aagagaaatg
    10741 caaacagtgc aaaaaatatt tgaacatcat gggtttgaag tattttctgt tatatgtaaa
    10801 aatggggcaa tgcctaagag tacaattgga ctagaagcat ctcaaacaat atcaaagtgt
    10861 tcagatgagg taatgtgcaa tccaatagga caagctttat ttttaaatga agctaaggtt
    10921 gaccttaatg taattcttgg attatgtgtt ggtcacgata ctttagccat gaaatatatg
    10981 gaagctccta taacaacttt agttgtcaaa gatagagtaa ctgggcataa tccagttgct
    11041 gcaatatata atgcagaatc atattttgag aaaaaattgt ttgataaaga tgaagaataa
    11101 ttcaaagact tattgacata aaaagcaatt agtggtttaa ttataaaaaa gataaaactt
    11161 taaattatcc tatagataca taaagttata taaataaaga aaaatattac agctaaattc
    11221 cttaaagtag tccagagaga ctaaagggtt atagataatg ttagtatata acctctagta
    11281 aatcttctag aggtttttga tgagtataaa ataaaaattt aaatataata aagtctttta
    11341 aactagtcca gagagactgg gaaagatatc tcatagctaa tgaatagagt tatgtataga
    11401 taaaatcctt atgcctcttt tggtgtgagg atttttttat gattatttag gctttattaa
    11461 aaaggagatg taaaaatgat aaatggtaaa gaaaaattga tagttgcaat tgatactgac
    11521 gaatttgata aagctaaaga actaatagac agattagaag atagtgtaga catatttaag
    11581 gtagggctag agcaatacgt tgccacaaaa ggtaaaacaa tagattatct taaagaaaaa
    11641 gggaaaaaaa tatttttaga ccttaaattc catgatatac caaatactat gaaaagtgca
    11701 gtgagagctg cagtgagaga taatgtttgg ttaatgacaa tacatgtatc agatatggaa
    11761 ggaatgagac aatgtgcact aattgctagg gaagaagcag aaagattaaa tatagaaaag
    11821 ccattagtag ttggagtaac agttttaact tcattaagca accaagattt acaggacata
    11881 ggatgtaata tgacaacaga agagttagct ataaagagag ctaaactggc aaaagaatca
    11941 ggaatagatg gggttgtttg ttcagcgcaa gaagtagata aaatagtaga agcatgtgga
    12001 actgatttta taacagtttg cccaggaata agacctaaat cagcagaggt tggagaccaa
    12061 aaaagagttg taactccatc agatgcaata aaaaaaggtg ctcattattt agttgttgga
    12121 agacctataa caaaagcaga aaatccaaga gaatcagcta ttaatattgt aagagaaata
    12181 gaaaatgctt agataaaata tttgggaggt agcttatatg aaggggaaat taattttaga
    12241 agatggaaca gtatttgaag gaaaagcatt tggatatcta aaggaagcta ttggagaagt
    12301 tgtatttaat acttcaatgg ctgggtatgg agaactattg acagatgcaa cttattatgg
    12361 acaaatagtt actatgactt atccacttat aggaaactat ggaataaatt tagaagatgt
    12421 tgaatcaaat aaggtgcaag taaaaggact tattgtaaga gaaaaaagtg ataacccaaa
    12481 taattttaga tgtgaaatgg atatagacac atatctaaag caaaataagg taataggact
    12541 tgaaggaata gatacaagag cattaactaa aattattaga aacaatggaa ctaagagagg
    12601 aattataact ttagaaaatg tatcattaca agatgttcag tctaaattag aagagcattg
    12661 taacaaagat tcagtaaaaa atgttacaag aaaagaaatt gaaaatataa aaggtactgg
    12721 agctaaagtt gtagttatag actttggtat taaaaatagt gtactagagt ctcttaaagc
    12781 ttgtaactgt gatataacta tagtaccagc aatgactaca gtagaagaaa ttttaaatat
    12841 aaatcctgac ttaatcttat tatctaatgg cccaggaaat ccagaggata taaatgatat
    12901 agttgaaaat ataaaaaaat taataggcaa aaagccattg acaggaatag gtttaggaca
    12961 tcaaatttta gcattggcat taggaggaaa gacttcaaag cttgtatttg gacatagagg
    13021 aggtaatcac ccagttaaat gcttagaaac tggaaaggta tttataactt ctcaaaatca
    13081 tggatataat gtttcagaaa tgccagaaaa tacagaagta actcacataa atttaaatga
    13141 caatactata gaaggaatga gacataagga attaccaata tatagtgttc aatacattcc
    13201 agaatacaca acaaaagaag atttagattg tatatttaat aaattcttag cattaatata
    13261 gatttagtat tatttacaga tagtaaaaac catttagata aggagaaaaa tttatgccta
    13321 aattagatag tataaagaaa actttagtat tagggtcagg accaataata ataggtcaag
    13381 ctgcagagtt tgactattca ggaacacaag cttgccagtc tttgaaagag gaaggaattg
    13441 aagttgtatt aataaatagt aatccagcca ctataatgac tgataaagaa gtggcagata
    13501 aaatatatat agaaccatta acaatagaat ttatagaaaa aataatagaa aaagaaagac
    13561 cagacagttt acttgcaggt atgggtggac aaacagggct aaatctagca gttgaacttt
    13621 atgaaaaagg catactagat aaatatggag taaagataat aggtacttca gtagaatcta
    13681 tcaaaaaagg tgaagataga gatatattta gagaagtaat gaaagaaata aatcagcctg
    13741 taatagttag tgatatagtt acagatttac aagcaggtct tgattatgca cttacaattg
    13801 gttatccagt agttgtaaga ccagcataca cactaggtgg aactggtggg ggtatagctg
    13861 ataatgagga agaattaaga gaaatacttt cacatggtct acaattaagt cctgttggac
    13921 aagtattaat tgaaaagagt ataaaaggtt ggaaagaaat agaatatgag gtaataagag
    13981 attcaaaggg aaattgtata gttgtatgta acatggaaaa tatagaccca gtcggagttc
    14041 atacaggaga cagtatagtt gttgctccaa ctcaaacttt aagtaacaaa gaatgtgcaa
    14101 tgcttaaaaa agcatcttta gatatattaa atgcagtaga agttcaaggt ggttgtaacg
    14161 ttcaatttgc acttaatcca catagttttg aatatgcagt tatagagata aatccaagag
    14221 ttagtagatc atcagcttta gcatcaaaag ctacaggata tccaatagct aaagtttcgt
    14281 caaaaatagc tttaggttat actttagatg agatagaaaa tgcagtaact aaaaaaactt
    14341 atgcttgttt tgaaccaact atagattatg tggtggcaaa aataccaaaa tggccttttg
    14401 ataaatttaa aaaagcaaat agaaaattag gaactaagat gatggcaact ggtgaaatta
    14461 tgagtatagg aagtaatttt gaggctgcta ttcttaaagg gataagatca cttgaaacag
    14521 gaaaatattc attagttcac acaccatctg aggagagaag catagaggaa ttaaagaaaa
    14581 gagttgtagt acctgatgat gagagactat ttgatttagc tgaaatgata agaagaggct
    14641 ataaagtaga aatgatagaa caaattacag gtatagataa atggtttata aataaattta
    14701 aatggatagt agagcaagaa gaaaaattaa aagttatgaa aattgaagat ttaactaaag
    14761 actatcttct tgaattaaag aaaaagggat tctctgataa aggtatatct gacctaatga
    14821 agataagtcc agaaaaatta tatgaattga gaagtctata caatataaaa ccagcatata
    14881 aaatggttga tacttgtgca ggtgagatag atgctatatc tccttactat tattcaactt
    14941 atgagcaata tgatgaggta gttgttagtg ataagaaaaa ggtgatagta ttaggttctg
    15001 gtccaataag aataggtcaa ggtatagaat ttgactattg ttcagttcac tgtgtaaaat
    15061 ctttaagaaa aatggacata gaaactataa tcataaacaa taaccctgaa actgtaagta
    15121 cagactttga tacatcagat aagctgtatt ttgaaccatt aacagaagaa gaagttttaa
    15181 gtataataga aaaagaaaag ccagaaggtg taatactaca gtttggtgga caaacagcta
    15241 taaaattggc taagttttta catgaaaaaa atatacctat actgggaaca gattttgatg
    15301 atatagatgc agcagaagat agagaaaaat ttgatgattt actagagaga ctagatataa
    15361 atagaccaaa aggaaagggt gtatggtcac ttaacgaagg tattgaagtt gctaatgagt
    15421 taggttatcc agtattagtt agaccatctt atgtacttgg tggtcaaggt atggaaataa
    15481 cttataacga agaaaaatta tctcaatacc tacaagatgc atttgataga gaccataaaa
    15541 atccagtact tatagataaa tatctaactg gtagagaaat agaagtagat gctatatgtg
    15601 ataaggaaga tatattgata cctggtataa tggaacattt agaaagagct ggagttcact
    15661 ctggagatag tacaacaatg tatccaagtc aaaatatatc tgatgaaata aaagaaaaga
    15721 tagtagaata cactaaaaaa atagctttag accttaatgt gcttggtatg gttaatatac
    15781 agtttataga atttcaaaat gaattatata taattgaggt taaccctaga gctagtagaa
    15841 cagttccata tataagtaaa gtaagtggag taccaatagt tgacttggct actaagtgta
    15901 tgttaggagc taaattaaag gatttaggat atggtacagg agtttacaaa gagccaaaat
    15961 tagtatcagt aaaagtacca gtattctcaa tgtctaaatt atctaaagtt gaagtaagtc
    16021 taggacctga aatgaagtct acaggagaag tcctaggtgt aggagaaaat ttagaagaag
    16081 ccttatataa agggttcttg gcagcaggta gacatatgtc tgatgaaaga ggagtagtac
    16141 ttgctactgt aaataatcat gataaagatg agtttataga gatagcaaaa gatatgaaag
    16201 aattaggata tactttt
//
LOCUS       DAKBNN010000080        16097 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000080 DAKBNN010000000
VERSION     DAKBNN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.080"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(223..603)
                     /locus_tag="OUB02_002128"
     CDS             complement(223..603)
                     /locus_tag="OUB02_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8885885.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(804..1997)
                     /locus_tag="OUB02_002129"
     CDS             complement(804..1997)
                     /locus_tag="OUB02_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCT8885886.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2060..3316)
                     /locus_tag="OUB02_002130"
     CDS             complement(2060..3316)
                     /locus_tag="OUB02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HCT8885887.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSEVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            complement(4655..5389)
                     /locus_tag="OUB02_002131"
     CDS             complement(4655..5389)
                     /locus_tag="OUB02_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8885888.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYN
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(6395..7033)
                     /locus_tag="OUB02_002132"
     CDS             complement(6395..7033)
                     /locus_tag="OUB02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCT8885889.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(7036..7893)
                     /locus_tag="OUB02_002133"
     CDS             complement(7036..7893)
                     /locus_tag="OUB02_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885890.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(7895..8269)
                     /locus_tag="OUB02_002134"
     CDS             complement(7895..8269)
                     /locus_tag="OUB02_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8885891.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(9448..10638)
                     /locus_tag="OUB02_002135"
     CDS             complement(9448..10638)
                     /locus_tag="OUB02_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCT8885892.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(10654..12609)
                     /locus_tag="OUB02_002136"
     CDS             complement(10654..12609)
                     /locus_tag="OUB02_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCT8885893.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKASTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSEPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(12626..14686)
                     /locus_tag="OUB02_002137"
     CDS             complement(12626..14686)
                     /locus_tag="OUB02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCT8885894.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKDAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVVDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNVCILEKDKYIIKGFEL
                     YQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQ
                     TTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGN
                     GLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVG
                     KKYPAVLAIHGGPRLTYGPYFMNEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFG
                     DIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIA
                     NWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDYRC
                     YMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKD
                     NIGQ"
     gene            complement(15002..15652)
                     /locus_tag="OUB02_002138"
     CDS             complement(15002..15652)
                     /locus_tag="OUB02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8885895.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT         5514 a         2826 c         1781 g         5976 t
ORIGIN      
        1 gaatagaaac aagttctaga ttactataga gatgacatat caaaatattt ttaaaaagta
       61 atttaagtat tagaagtaat tgaagttagt aaatataaat gcgtttactt ttaagtattg
      121 cttaagggaa taaaaaataa aaataaactc tgcaaccatc taaaaatagt tgcagagctt
      181 acaaaaccaa aattatctaa attgtaataa aaataataac atttatacat aagcttcttc
      241 ccaatttact acccagtttt ctaacgactt tatcttttcc tctgttagct cctcttcgtg
      301 aaatgatact aataaattcc ttataaaatt attcttcaaa agattttgca catttcttga
      361 tataaactct aaatatgcct tttcttttat tgcaactgta taatacatac acttgcctgt
      421 ttcttgaaca tataagaatc ttttctttga tagttttgat aaagttttta aagctacttt
      481 ttctggtaaa ttatgttctt ctttcatata atcaataatc tcatatgaat tggtttttat
      541 atctaaattc caaataaatt tcataataac taactctaat tttgataatt tttttaaaca
      601 catagttatc actccgcttc tttatctata taattaaata ttacaataat aatttcacaa
      661 aatcaattct aataatcata acatacctta aaatctgttc ttaacttcta ccttcttaat
      721 ctcccaaatg atttttttat attgtattca atagtataat ccttttccaa aatataactt
      781 accagttaaa ataatcttta tttttactta ctctcgcctt ttacagaagt taataatttt
      841 ttataagtta catcaattcc aaaagctcca agaggtgcag ttataagtat agctagaact
      901 gctaacgtca aaataagttg tccagatgcc atccccattg caagtggtac acctcctatt
      961 gctgcctgta ctgtagcctt tggaatatag ccaatcatac agaacaacct ttcctttatt
     1021 gtcaaatttg tcttaataag gcagaagaat actcctacca tacggaataa aagaactcca
     1081 aatattaaaa ttacagctcc aattcctgct ttaaatgcat aacttatatc tactgttgcc
     1141 cctaccaaaa caaacaagag aatttcagca ccaacccata gtttcgaata ctttatagac
     1201 aatcttcttg caactacacg atattttatc tgaaggaaaa tcccaatact cataactgct
     1261 aataatccag aaaatggcac aataccctct aaagcagtct ctaaacttat cattaaaaat
     1321 gagatactta aaagcaacac ccctttagca gaatctctca tatgaacctt cttaaaaaat
     1381 actgctaaac agattccaat cactgctcca gctattacac ctaatataat tgatactgga
     1441 atttgtaaaa aactaatagc tgatatacta tttccttgtg ccaaccctgt gaatactgta
     1501 aacatcacaa ttacgaaaat atcgtctata gaagttcctg ctaaaagcat ctgtggaata
     1561 cttttttctg taccataacc ttcttccatt aatttaagca ttttaggtac aataattgcg
     1621 ggagaaactg ctcctactac tgcacccatg actgctgctt ccaatataga aacgcctagt
     1681 aactttggtg ctaaaactat cattccgata atttcaaaag tagctggaat aaagcacata
     1741 agtacagcag gtcttcccac tttcttcaaa tcgtttatat ctaaagataa tccagccctt
     1801 gttaatataa taatcaatgc aatctttcta aggtcagaag aaatatctag tattgaatta
     1861 tcaatcaaat ttaaaacata tggacctaat ataattcctg taagtaacat tcccaataag
     1921 ttaggtaact ttatcctctt aaaaatacct cccgaggcca ttcctaataa aaaaatcaaa
     1981 gctaaacttg tcaacatatt cctaccctat cctttctcta atattctttc tcttctaatc
     2041 atacattatg ttgttttatt tattgttttg ttgaaatttt ttgtagtgaa taaatagatt
     2101 tatccatatt aaatatatat gaaatagcgc atactacaaa gaaataaggt aaatagctgt
     2161 acccaaaaac ttctgcccct ataaatacag gtgcaaaaaa agtatttgta gcactaccaa
     2221 agacactagc atagccaagc gctgcaacta attcaattgg caaattaaaa aatcctgcta
     2281 acaatacacc aagactagct ccaatggaaa acaaaggtgt aacctctcct ccttgaaaac
     2341 ctgctgacaa agtcagtatt gtaagtataa actttaacag ccagtcaaat gaataaattt
     2401 ctccaccata gaaactattt tgaattaaat ttgtccctaa acctgaatat cttcctttat
     2461 aaaataaaag gaataataca ctaagacata ctccaataat agcaatcctt atcataggat
     2521 tttttaatct attccctatt tttctttttg acaatttcag acaccatgca aatgctccac
     2581 caaccattcc aaaaataatt cctaaaacaa taagcttcca aaatattgac aagtcaaata
     2641 caacttcaga agaaagtgca aaagaaaatt tctctaaccc taaagctttt gatactgcac
     2701 ttgctgtaaa agaagcagta aacgcaggaa aaagcgattg atactccagt gaaccagcaa
     2761 ccaaaacctc catagcaaat agtattgctg ctattggtgt ctcaaataat cctgcaaaac
     2821 cagctgccat accagttact agaaaaatac tagaagcatt ttttataggc aaccttttac
     2881 ctacccaatg tgaaaaagtt gcaccaatct gaacagcaac tccttctcgt cctgcactac
     2941 caccaaataa atgggttaac catgtacctg atattataaa tggaaccaat cttaaaggaa
     3001 taatttcttc ttctccatgc cctacctcaa atattagatt cattccttta ctactttttc
     3061 ctccaaattt aaaatagcaa taagcaataa caactcctac tactgctaaa aatggtatca
     3121 gatacattgg atatgtttct cgaatatctg taacttttag aagaaccgtt ccaaatatgg
     3181 tgtctataag acctataatt gcaccaactg gtatacctat gagtcctaaa aaaaatagtc
     3241 caccatatgt tttataaacg cttttaattc ttattatcat tttaatccat attaaagttt
     3301 taaatttttc tatcatttta attcctttct atttgcatca taagattttt tgaaagtttt
     3361 attgcatcaa ctgtagtcaa tgtaatgact atcataattg ttttttaaat tgataggata
     3421 taggtacact cttaacccat tcttaataaa ataattaaca tataatctac tattttttct
     3481 attaattaaa tatttcttca aatttaaaaa ataaaaattt agtaagtact tcacctccat
     3541 tttattgatt taaacaacaa aaaaagctgg tggcatctat gatatcatag aagtcatcag
     3601 ctcattggca gtttaggata ttatcatcca agggagaacc tcattcccat aattattata
     3661 taatttttta tttttcatat gtctatttta tgtaaattgt atttttaatg ttataaataa
     3721 tataacctat caattaaaat attaaagtct ctaatattat gagtgttcaa tatttcatat
     3781 tatttttatg atatataatt taaatgattt tctttacttt gtattatgat acataactta
     3841 aatatttttc ctcactccac attatgatat attatttaaa tatttttctt cactctgctt
     3901 atattatgat acatgattta aatattttct tcactctgct tatattatga tacatgattt
     3961 aaatattttt actcactcta cattatgata tattatttaa atttttttct tcactctgct
     4021 tatattatga tacataattt aaacattttt cttcactttg cattatgata tatggtttaa
     4081 ataatctttt ttactttgta ttatgataca tgatttaaat atttttacgc actttatatt
     4141 ataatacatg ctttaaataa tcttattcgc tttgtattat aacacattat ttaaatattt
     4201 ttactcactc tgcattatgg tacattattt aaatattttt cttcgctttg cattatgata
     4261 catgatttaa atatttttac tcactttgta ttatggtaca ttatctaaat atttttcttc
     4321 actctgtatt atgatatata acttaaatat tttacctcac tctacaatat tatatataat
     4381 ttaaatattt ttcttcactt tgtatactaa agtttattta aagtattttc tttcccatta
     4441 tactatgctg ttaaatattt ttcaactgaa gtattgtaga accttaaaat tactataaaa
     4501 aactatattc taaaatactt gtaatactac atatcaccct taacacttaa gtataatttt
     4561 aaagcaaaat tcttattact tatatttaat tttgattcta catctttaga tataatttta
     4621 agtaatttaa gaaattattg taaatcatat agtactactc aataattagg tttttcaatg
     4681 ttccaattat gtttctttga tataatccac catgactaaa aactatcgtt tctatattat
     4741 aattacttat atttttaata gcatctttta actcttgttt gttgctgtac atcacatcaa
     4801 caggcttaag ttctccattt tctacctgta acaaatctcc agcaataagt actttagact
     4861 cttttaaata aagacatatg tgacctctag tgtgacctcc tgtatgtata acttctattt
     4921 cctcacctag attcaatttt tcatggttat ctaatgacac atctacttta gtatatgatg
     4981 atttaaatcc aagtcccata acatttagca tactaagcat cttatcatca attttatcta
     5041 ccatttgttc caacatataa agtttaaaag gagtttcttc tcctgttata tagctgactt
     5101 cacttttgta tgcatatact ttgatattat tcttagattt ttctcttaga taatttatgt
     5161 ttcctatatg gtctatatca tgatgagtaa ttattatatt ttttaaccta tcaaagctcg
     5221 tgttactttt attaagagca tcaattatta aatcaccttg acccggaaga cctgtgtcaa
     5281 taagtgttgc atcatcttct gtgtagatta caggaatata cataacttta tcttcaccga
     5341 atacatttga tgaaattttt aaaacatcta atccctttgc taaattcata atttttctcc
     5401 ttataattac ttaattagag gtcaatttaa ttaaagaaat tgagaaaatg tagattttcc
     5461 tctagttata ttttattaat taaaaattat taatacctag tatatcactt aagttgaagg
     5521 attactcgac ttgatttttt tgttatttta tggtatagta attaatatag aagtaagtaa
     5581 ttgcaagctt tgcaattact tttatttttt tctaataact attttatttt tgataattcg
     5641 tataaaatta aaagtaaagt aatatggaaa atatgaatat agaaaaactt taataagaaa
     5701 ataaattatg aataaataaa agaaacgcaa tactgtatat agatgattat ttagaaaaat
     5761 aaaatctctt ttaataaaaa taggcttcaa aataaacttt tagttcataa agcttaggag
     5821 taactaaatt gagtgcaagt gtatctactg ttcaaaataa atagttcata aagcgctctt
     5881 ttttatatta aaccaattat attttcgtca tttcagcaat gaaaaagaag cttatctcta
     5941 ttttgagaca gcttcttttt attaacttcc tactttttat tagttttcta aaattgaaaa
     6001 gctttattta taggattcct tagatttata ttttgacgat ataatttttt aaaaacatct
     6061 taaaaaatgg tatacaatac acacaatcat actcataatt gaaaaataat caatggaaaa
     6121 taaaattact ggatattatc tattaaaaaa tacaaattga ttcacaagaa acatataatc
     6181 tataatatta tgatgttact ctgccacacc aagtaacttt attttcaagt aaccttttca
     6241 taccatctaa ctttactcaa taaaaatggc aatataaata tgtactctat tttttacttt
     6301 taaatatctg acaagcttta tatactattg gaagtaagat acaaagtaaa acagcccaat
     6361 ctggcaaatg tggcatataa aagtccccct taccttatac aagctcctta ttactaaaaa
     6421 ttttaataga aataaacatt gaagctaaca gtataattaa acttaaaagg aacaatacaa
     6481 tattcactat catggatgga tactgatata taaatggaat ttcaaaattg ctgtgagcta
     6541 tagttaatgg taatacaaat gatgaagcca tgattatcac cacgaaaaag aattttgttg
     6601 tttcatatcc taatttatat tgaataggca tataaactcc ataaataata gaacttatta
     6661 ataaaacaat caaaatatat gtcaagtcaa aagttcctat ttgtggaata agtaaatttt
     6721 caatccagta aactaatgta caatagataa ataccattgc aaataaaaca tacttagatt
     6781 gaactaagcc acgtctagga taaggagttg aacaaagtaa tacagatgct tttgaatatt
     6841 gactctcctt taataataga tactgacaca gcataaattc tgaaaatata attgatagta
     6901 caaatcccaa tatttgtgca aatggaggta aacgccataa tataaataat gggattccta
     6961 ttgcaataaa taatatcaac aatacatatt tttttacaat cagaatatct tttttaacta
     7021 aatgaatcaa cataattact ctctcctttc aatatatgca agcataatat cttcaattgt
     7081 aggacgctct attaaaatat ctggcatatt tttttgcact tcattaattt ttgaggtaat
     7141 ccctataaaa ccataacctg taatttctaa atttaaaaat aattttttgg tttcatcatt
     7201 tagaaattga atatcccctt ttacttttct atgttcgtcc aatagagtat ctttttcacg
     7261 ctctaatata atacgtccat tgtcaatcat aacaatcata tctgctattt tgtctaaatc
     7321 agatgttata tgagtggaaa agaaaacacc acgaccacat ttcttcatat atcctgttag
     7381 tatgtcaagt atctgactac ggattagtgg gtctaaacca cttgtaggct catccataat
     7441 taaaaactca gctttatgcg ataatgctaa tgctaaagca aatttcattt tcattccttt
     7501 tgaaagtgta tcaatctttt gttttgggtt aagattaaaa cgcttcatat tttgttgata
     7561 atcttcttca caccaattat catatgaagg tgcaataata cttttcattt ctgccattga
     7621 caactcctca tagaaatatg aatcatcaaa tacaactcca attcgatttt ttaattcctt
     7681 ctcatttcca tcgaaatctt taccaaagat ttttattttt ccactatcac gctttacgac
     7741 atccaatatc gaacggattg ttgttgtttt tcctgctcca ttaacaccta taaatccagt
     7801 tatacaatct tctggtaatg caaagcttat attagataat gaaaaatcgg tataagtctt
     7861 atttaagtta taaacttcta aaatattttc catattaatc ctcctgatat agaatatcca
     7921 tcatttcttg taactctatc tgtgtaattc ctagtgcttt tgcatcctta atcatatctg
     7981 ccattttttg ttcaactata cgtctctttg agtctcttag tatttctaaa tttccagttg
     8041 aaataaatgt tcctattcca acttggcgtg taagaaagcc ttcattttca agttcttcat
     8101 acactcgtct aatagtcaaa atactaactt ttaaatcatt agcaaaattt cttatggatg
     8161 gcattgcatc cccttctttc agttcttcag taaatattgc ttctttaatt tgctctttaa
     8221 tttgttggta taatggctta tctgatgtgt tagaaatgag cactttcatt ttttatcacc
     8281 ccctttttat cattttcagt gtgatgttgt tatatacaca ctatacactt agagcacttt
     8341 tttgtcaata taaaagatgt aaaatattac tatgacaaaa taaatatgaa tgaaatatta
     8401 gagatttttg atagctataa acacttttta aaaccacata agataggatg tattaaatat
     8461 ttattaaaag taagaattga atatctaaaa aaaagaggaa tcctgtatga atgatttgtt
     8521 tgatgataaa ttaagttata aactatttat aaaaaacata aaggaataaa tccatttcta
     8581 attacaaaca tactaaaaaa tttagatagt aataaataga tagtaataaa tgggatgaat
     8641 ttataagaat ttggatagag gaaagagtta ttcaagagtg tagactagag gtaaactcta
     8701 tattaaaaaa atttaaattt ggatattatg atgcatggaa tatttgcaga agtaaccttg
     8761 ctataagtat agaagatttt tttgattaaa aaagataata ctctaaaatg tgagaatttc
     8821 aacattcaat actgtgcttt aaataatatc actgcaaaat accatccacc ttttaccata
     8881 attgaatacc ctaattattt taaaaaagaa aacattagaa acggaaaacc ttgtatatat
     8941 aaaataaaaa atactaactt ctaaaacacg aatactcttt gataaataaa acaaaaaata
     9001 attttaataa ttaaaattgg ctatctcaaa ctagagataa gtctctttct cacttttaaa
     9061 actttgaaaa tatattcgat ttcgtacaaa aaagagtgcc ttagaactaa aaagttcact
     9121 ttgtggcact cccttttttc tcctcttttt aattctttct aaagattttc caaaaatatt
     9181 attaaatacc ctatttatag ataaatcaag ttaaagtttt gttttagaaa ataaaaataa
     9241 actgtaacta ttagaatagc aaataaaaaa tctgtttctt gataaaatca agattataat
     9301 gctaaaaaga aaatattctt ataataaatt aactaatata aaattcgttt ataaattaat
     9361 actgactttc tagctttatt tataaattaa tactactttt tagcttctcc tataaattaa
     9421 caccactttt ttaactatga aacctatcta caacattggt accataagac cagctaccac
     9481 aatagaaatc atacaagcca taccaccaac taccagctta ggcataatct tagacatcaa
     9541 atatgctctt tcttcttccg tttctccaac agcattacac acttcatttg aaattaaata
     9601 tgtagttggg aatcccaaca tctgacaaaa ccctactcca aaagcaagag gtttagaacc
     9661 gataattttc caacctggta acaccttcat cataaggaat ataccaatga tagaagctgc
     9721 aaacatacct acaattggta caaaaagact aattaaatct gtcaaagaaa cttttgctaa
     9781 agaaggaata acagctgcaa acacaaccat gttgataaat cccttgattt ttcctttttc
     9841 caaaatgtct tttggtacaa taccaatacg tgttacaata acaccaacaa caagacatac
     9901 aatagaataa tttacatgaa taatctctga aagttcatac cctagataag agaataatgc
     9961 tataaaaaat atacaaacat ttgtagaata atatttatcg tatttttcac agaaagcttg
    10021 ttttactttt gtgcctgtat tctctgcttc tttcttatca gtatcaacat ttgctaaatc
    10081 aatgcctttt gattttgctt catgatattc tactaaaaga cggtttgcct cttgtaacgc
    10141 tgctcttgaa gctattggtg taccaataaa cttctgaatt gcaaatacaa ctgttgctgt
    10201 agctgctgca agtgtcagtc ctttttctag tgctgcttgt gtcataatag tagtagcagg
    10261 taaagctcca tttataactg gtatgcttgt caatactgtt gattttccaa acaaaggaat
    10321 acataatgag acacagacta taactgctac aattccaagc catgctgtca atactgttct
    10381 ccattcgtct ataaattgtt taacattcaa cattgttccc atactaaaca taatcattgg
    10441 aacactccaa ctagctgctg cggtcatacc tgctttttca ataatatcag ctggtattgc
    10501 ttttgtgaca aatagaatca aaaataaaag tagtgtaaca aatacagctg atacttttgc
    10561 ttttgttttg cttgcaatta aatccccaat agcaaataaa actaatacga cactaaacca
    10621 taataacata tcactcatag aataaccccc ttattataaa tatctttcaa accaatctac
    10681 aatagcattt gctcttgcaa ttctattaga tggtcttcca tttctagaca atccatgtgt
    10741 atctccgtgg aacaaataca tttctgtatc tacaccaagc tgttttaatg ctgtaaacat
    10801 ttggaaagta tcccccataa aacatctata atccctatca ctatgtaaga acaatgttgg
    10861 tgtcttaaca tttttcacat atttaatagg tgaatgattc caaagaacct cctcatgatt
    10921 ccaaggcgtc ttatctccac aagtctgctt cataattcta tccccaaagt caataattaa
    10981 agaagctgta atcaagttac taatacctct ttgagaaact gctgctttga aacggtctgt
    11041 atgaccaatt atccagtttg tcatataacc accgtaactt ccccctgcaa cccctaagcg
    11101 gtctgcatca atcccaggat atttttcaat acaagtatct acaaattcca taagatgctc
    11161 atagtcaaca tacccccatc tttctaccat ttctaagaat ttttcaccat aaccgtcacc
    11221 accatgaggg ttacaataaa tcacatacat tccctttgat gttacacact gcttcaagtg
    11281 tgtaaacata tagccatatg cccatttagg tccaccatga atgaacaata cagcaggata
    11341 tttttcgcct tctttagcat ctataggcgg tataacaaaa ccatcaactt cttctttatt
    11401 ttttgctaca aacgtcaatt tttcaggtaa tgaaacctta tgagtatcta aatatggttc
    11461 agaaaaagca gtcatttttt tcattgtacc tgtagtcata tcaatgctat atatttctgc
    11521 taactcataa tctttcaaca ttactgtgta catggtgttg tcgtaaatat caaaagatgt
    11581 aaaagcatct acttttgtga tgatacgtat attacctgtc tttgtatcaa actcattaaa
    11641 ttctgtcata gttctgcaaa ttcttgcacc aataaatttg tctcctataa ctttattact
    11701 acgtccacca ccatatactg catctacatc cagtaatgta gcatacatac catctggact
    11761 agcaatcata tttatttcac tagtactcaa atcaagtata aaaatatcat gattctgtgt
    11821 aatagaacgg tctaatgttg aagcttgcaa cattacttta tctttgccaa tatattttgc
    11881 caaacttatt tggtactttc cttgtggtac aagttctttg gtttcaccac tgtctatatg
    11941 atactcccaa agtccagaaa aaggagttgc acaatcttca taaatctgac cagtatataa
    12001 aattctgttt ttatcagaag aaacgtctac acctgctact tcacaaaatt ttggtgtaat
    12061 ggcttttgtt atattttcac cttcttgata taattctaat gttacacgtc ttctttgaga
    12121 aaaattttct ccatctctta taaaaggttt atctgtaaat gtccaataat ctacaccctc
    12181 tactgcccta tctggttctg attttttaga atccgttgga ttttttgttg tcagtaacca
    12241 caatttattt ccaagtgata caggtacgct acaagctttt ggaatagaaa atgctttttt
    12301 tgcttctcct ccttctatag aaatagtgta aaaatctgtt gtacttgctt ttggcttatt
    12361 tcttttagat gtaaaaagta gttctgtatt agaaatccaa ctaaatgatg ttacatcttt
    12421 tgtttgagtc atatgtattt gtttttctgt tttgatgtcc atcacatata agtcatgttc
    12481 atagcagtct ttttcctcgt tgatatttga tataatataa gcaatatgct ttccatcagg
    12541 tgaacaagaa atatcatgag ggaatatata ttcaaaaata tctgaacttt caatctttat
    12601 tttttccatt tttatattct cctttctact gtcctatgtt atcttttttt atatataccg
    12661 aaaaccaatc tagcatttca actaatcttt gtagcttttg ttttggtcta ccagaacgag
    12721 acagttcatg gttttctcct tcaaatagac aaagttttgt aggacaccca tgaattttca
    12781 atgctgaaaa catctggaat gcttcttgca tatagcatct atagtctttt tcaccatgta
    12841 aaaataaagt cggtgtaaca catttatcag cataagcaag aggtgactgt ttccattgaa
    12901 gctcgtggtt ttccatatga gaagcacctg tttgtccttt gcagaaataa taaccaatat
    12961 ctgttgtacc ctccatgcca ctccagtttg caatagaacg ctgagcacaa gcaacagcaa
    13021 aacggtttgt atgaccaatt atccaattcg tcataaatcc accataactt ccaccttcaa
    13081 cagccaattt tgttttatca atgtctgtat atttttcaag tacagtatct gtaaattcca
    13141 taaaatctat ataatcaata tctccaaact gtttcctaat atctgcaaaa gcattgccac
    13201 gaccttcaga cccccttggg ttacagaaga atacaaagta acctgcactt gtaaatacct
    13261 gtatctcatt cataaaatat ggtccatatg tcaatcttgg tcctccatga atcgccaata
    13321 cagctggata ttttttcccc acctcataat ctactggttt atatacgtat ccttgtattt
    13381 cccatttaga acttcctttt gactctatgt attctggcac agaaatttta tattcctcta
    13441 aaatatggtc atttaaatgt gttttcttac acaatgttcc attctctact gtataaactt
    13501 ctggtaatcc atttcctaaa caacccacac atacaatatt gccatctttt acatctataa
    13561 aatcaactga acctgtctgt gttacttttt cctgttttcc tgttggaata tgtattcttt
    13621 caataatgca gtccatatct actgtagaag tgtaataaat atattctcca tctacctttg
    13681 ttgtctgacc agcacccatt ttaacatcag atgttactgt atttccaata caatgatgtt
    13741 gatgccctga aagaagtttc atttctttcg tatcaatatc aatagtataa aaatctccat
    13801 tttctccatt tccataagat aaagcatcag ttaggtttag tatcacttga ttttgatata
    13861 attcaaaacc ttttataata tacttatctt tttctaatat acagacattt gtgtttgtat
    13921 caaatacata gaccccattt ttcaaacctt ttacatcttt aaactcttgc cctatatata
    13981 agatttttcc atcacttatt ttcataccta caacattaaa cataggtgct gtaattgcct
    14041 tcaactcatt tatattactg tcgtatacat ataggcgttt tctttttctg ctaatatatc
    14101 cctccccatt gacagcataa ggaatttctt tgaaatgttc ataatgtctt ttgttataag
    14161 catttatttt tttctgttgt tcttcttctg gcaatccctc tacaataggt cggtcattgt
    14221 cataagaatc aacaactgca tacaaacctt ttcctatagg attcaattta ccacctttga
    14281 tagggagtct aaaaagttct tctgcttctc caccatttaa agataaagca tagtaactca
    14341 tgcattgctt accgtttttg acagcatctt ttactttttc atttcgcaaa gcagggaaca
    14401 agatagtatt ttcatcttcc caggcatatg ctccaacaga gtttatagac gttatttgtt
    14461 ttaattcgcc agttttattg tttaatatat aaacttggct tgtgtaacca ttctctttga
    14521 tatcagcttt ttttacaata aatgctgtat atcctccatt tggtgatatt tggcaatcac
    14581 ttataaattg atattccaaa aatgtttcac tcgtaaatag ctttttcata gtattttcta
    14641 tcctttcttt agtaattttt ttcaaactcc ctaccacctc tttcattttt tattttaaat
    14701 ttaaaataaa aaattttaaa tttaaaataa aaaattttat aaaggttatt tttttaggaa
    14761 gccttctggc ttccttgtaa tatttgtaat ttaaagtata tttaatccat gcttaattca
    14821 tacttaatct atacttagtc catacttaat ctatatttaa ttcatactta gacttattta
    14881 cactatattt agattatatt tattagatta tatttagatt atgtttaaac tataattaga
    14941 ttatacttag actacattta gcaacttaat agataagtat atatagtaac ttcaaaactt
    15001 attattcatc ttcacagaat cttttaatag ttccttgaat atgatttctt agtttatcta
    15061 ctgctttttt tgtatctctg tcttcaatga attttaatat ttcaaaatgg tcttgtactg
    15121 ttccttcata attacgattt tttaatggtc gaattaaata gttcactact ataaatcttc
    15181 cttgtatacc aacatataca tcatttaaaa tctcattatt tgccccttct acaatggctt
    15241 tatgtaattc caaatctgct agtgcaagtt ctacagattt ttcttgacga tagtactcct
    15301 cgcatttatc agagtatctt tttattacat ctagcttttg ttgaaaaacc tcttcctctt
    15361 cttgcatcat tttttctaaa gcatatgttt caatcagtgt tctcgtttca caaacttgtt
    15421 ttaccatatc atattcatac tctggtacgt acactttacc atttgtcata atagtaagca
    15481 ctccatcttt atttagcatt tttactgctt gtacaactgg tgttctgctt acttcaaatt
    15541 tttcacaaag gtcatttagt aatatttttt gacctacaga gtattcacca cgaataagac
    15601 tttcaataat cttatgatat aaaatttcat ctaaatttga ctttactttc actactctca
    15661 ccttatttct attcttttgc catattatat tgaattactg gactttaatt acttttagta
    15721 atatttttaa agtagaaatt cttatttgat agtacttttg tactacaatt taaattattg
    15781 tactgtaaat catgctctta tactatagat taattcattg tattgcaaat caagttattg
    15841 tactatagat taaatcgttg tacttcaaat taaactatta tactatagat taaaccattg
    15901 tacttcaaat taaactatta tactatagat taaatcgttg tacttcaaat taaactatta
    15961 tactatagat taattcattg tacttcaaat taaactatta taccatagat taattcattg
    16021 tattgcaaat caagttattg tactatagat taaatcgttg tacttcaaat caagttattg
    16081 tactatagat taaatcg
//
LOCUS       DAKBNN010000081        16093 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000081 DAKBNN010000000
VERSION     DAKBNN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.081"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            43..1233
                     /gene="dltD"
                     /locus_tag="OUB02_002139"
     CDS             43..1233
                     /gene="dltD"
                     /locus_tag="OUB02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCT8885896.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            1354..2868
                     /locus_tag="OUB02_002140"
     CDS             1354..2868
                     /locus_tag="OUB02_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HCT8885897.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGRIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            2868..4016
                     /gene="dltB"
                     /locus_tag="OUB02_002141"
     CDS             2868..4016
                     /gene="dltB"
                     /locus_tag="OUB02_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCT8885898.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPSVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEIIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            4035..4265
                     /gene="dltC"
                     /locus_tag="OUB02_002142"
     CDS             4035..4265
                     /gene="dltC"
                     /locus_tag="OUB02_002142"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCT8885899.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            4482..5267
                     /locus_tag="OUB02_002143"
     CDS             4482..5267
                     /locus_tag="OUB02_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT8885900.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            5546..7456
                     /gene="phnW"
                     /locus_tag="OUB02_002144"
     CDS             5546..7456
                     /gene="phnW"
                     /locus_tag="OUB02_002144"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCT8885901.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDIEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            7815..8636
                     /locus_tag="OUB02_002145"
     CDS             7815..8636
                     /locus_tag="OUB02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885902.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            8677..9444
                     /locus_tag="OUB02_002146"
     CDS             8677..9444
                     /locus_tag="OUB02_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HCT8885903.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVVNIEYSDNLSVSDNSTVKNSIKVG"
     gene            9452..10072
                     /locus_tag="OUB02_002147"
     CDS             9452..10072
                     /locus_tag="OUB02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HCT8885904.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(10372..10959)
                     /locus_tag="OUB02_002148"
     CDS             complement(10372..10959)
                     /locus_tag="OUB02_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HCT8885905.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(11045..11482)
                     /locus_tag="OUB02_002149"
     CDS             complement(11045..11482)
                     /locus_tag="OUB02_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8885906.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            11586..12584
                     /locus_tag="OUB02_002150"
     CDS             11586..12584
                     /locus_tag="OUB02_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HCT8885907.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(12704..13630)
                     /locus_tag="OUB02_002151"
     CDS             complement(12704..13630)
                     /locus_tag="OUB02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCT8885908.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            14016..15620
                     /locus_tag="OUB02_002152"
     CDS             14016..15620
                     /locus_tag="OUB02_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8885909.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         6371 a         1697 c         2752 g         5273 t
ORIGIN      
        1 taataataac ataaatttga atatttttag aggagagaaa atatgagaaa attgatatac
       61 tttattacac cttttataat aggcgtagta tttttatttg ggttagacaa atttcttgat
      121 tctaaaactg atgaactttt gagggagaaa aatctacttc caattatgga tgatacttta
      181 agtgatataa aagataaagg ggttacagca aataatcatt ttttaagaga aaaggatatt
      241 atgattttgg ggtcatcaga acttagtaat tcaacaaaac aacatccaaa gtactatttt
      301 aatacaaata ggagtaaaaa taaggtgttt gcaattggaa gagcctatac tcagacatta
      361 caagatgctg caatattagg aagtatgaat cctaatattg ataataagaa agttgtcttg
      421 ttaatttcta tgcaatggtt tatggaaaaa gacggagtca caagtcacca ttatcagtct
      481 agattttcac caatacaatt ttatcgattt ttagataatc caaaaatatc taaacaaaat
      541 aagattgaat atgcaaaaaa atcaagtaag ttattatggg gaagtgatga gtataaagca
      601 gaagcattat atgctaagct atatgagcct aagacatttc ctgaaaaagc tgaaaaggtg
      661 cttttagagc cttattttca aggaagaaag tattgtatag ctcttaaaga aaagggtatt
      721 ctttataaac gattaattaa gctagataaa aagagagcaa caaaaagaaa aagaccaata
      781 aattggtcat atgagagaaa gaaagccatt gaagatgcta aaaaaagagt tggtaaaaat
      841 ccactaaata ttgataagta ctattataaa gagcatttta aagatggtat agagcaatac
      901 aaaggaagag acaaagatgt aaatttatta acatctaagg agtttgaaaa ttataaattg
      961 atgctaaatg tatgtactga cttaggtatt aagccagttg ttgtattaat cccaagtatg
     1021 gataaattct ataatttgac tggtatttct gaaaaagaaa gaaatcagta ctacgataaa
     1081 gcacaaaaaa tagcagaaag caaaggcttt gaagttctaa accttaaaga taaagggtca
     1141 gataagtatt atttaaggga tgtaatgcat cttggtacga aaggatgggt agatgtctgt
     1201 gagagattat ttaaaatatt taaagaacaa taaagatttg ttaactagta tttgtttaac
     1261 tatggttata atattggcga taggagttct aacatttact ccatttaaag aattgccatt
     1321 tatttataac ggattttaaa ggagcattct tatatgaaaa ttattgaagg aataaaaaaa
     1381 tattcaaata cagatagaac agcactaatg tgtaatggag ataaactttc ttataaagat
     1441 ttaaatgaat attcagatgc tatatcagta tttctaaagg atgtatacaa agaagaagat
     1501 actcctatag taatttatgg aaataaagaa aacatgataa tggcatgtat gataggagct
     1561 ttaaaatcag gcagagcgta tgtgcctctt gatattagtt ttccaattga tagagttttt
     1621 gaagtcacta aagagataaa accaaaggta ttatttaact ttagtgatga aagaaatttt
     1681 ggagatatca atgtaattga tatggataaa ttaaattata taataaatga atatcaggga
     1741 aaatcattag ataaagaaaa ctgggtaaaa gatgatgaaa atgcatatat actatttact
     1801 tcaggaagta caggaaagcc aaaaggggtt caaataagtt caaacaattt agatagcttt
     1861 tcagattgga taagcccata tctaaacata gatggaagtg aaaaagtaat aatgaaccaa
     1921 ccagcatatt cttttgacct ttcagttaca acaatatacc ctggtcttat ccatggagct
     1981 acattatttt caatatcaaa agatgtatta gcagactata gagaactatt tagacaattt
     2041 agtatctcag atatagctgt atgggtgtca actccttctt ttgcaggagt atgtataact
     2101 gaaaaagagt ttaatagtaa aatgcttcct aaccttgagt caatgatatt cataggagaa
     2161 gcactttcaa aaaatcttac taaagaactt atgagtagat ttccaaacac aagaattatc
     2221 aatggatatg gaccaaccga ggctactgtt ggtgtaagtg taaatgatat gactcaaaag
     2281 gctatagatg atgaaaaaag tcttccagtt ggatacccta tgtcaaattg taaaataaaa
     2341 atacttgatg aagatggaaa tgagcttaaa gaaaatgaaa agggtgaaat tataataatt
     2401 ggaccatcag tttctaaagg ttacttcaat aataaagaaa agactgatga agtatttttc
     2461 tatgatgaaa tagatggtgt aaagtggaga gcttataaaa ctggagatat gggatatctt
     2521 ttagatggaa atatatatta ttgtggaaga aaagattttc agattaaatt aaatggattt
     2581 agaattgaaa tagaagatat agaaaataat ttgagaaaag tacataatgt aaaaaatgca
     2641 gtagttttac cagtatataa ggacgaaaaa attgcttact taaaaggtat tgttgagtta
     2701 aatgaaaaga atgatttaag taatattaaa aatgggagga taataaaaaa ggaacttgga
     2761 aagtatatac cttcatatat gattccaaga aatatttcaa taatctctga gtttccaaca
     2821 aatatcaatg gtaaaataga cagaaagaaa cttatggagg aaatttaatg attttttcac
     2881 aatatggaga ttatttttat ctttatatat tattattaac atcaatacca tcagtaattt
     2941 taggtctaat gggcaagaat ataaagtatt atggtatgtt ggcaagtttg tttatgatat
     3001 tcttaatagt aggaattgat gtccaactaa agtacttggt aatatttatt attcttgaaa
     3061 taataatagt taaaggatat gaatatgtaa gaagaaaaac taaaaataaa tacatatatt
     3121 ggggattctt gtttgcatca atgctcccaa taataataaa taaaatatct ccagttactt
     3181 cttttggaat aattggattt attggaatat catacttaaa tttcagaact atacaaatgg
     3241 ttattgaaat ttatgatgga gctataaaag aagttaaaat ttcaaagatg ctttatttta
     3301 tgttattttt tccaacttta agttctggac ctattgatag gtcaagaaga tttgaacagg
     3361 acttggaaaa acaaatctct agaaaagagt atatagaaga atacttactg ccaggaataa
     3421 aaaatattgt catgggagta ggttataagt ttgttatagc atttttgata aatacatatt
     3481 gggtatcaag aatacctaaa gatatgagtt ttataaatat tttaagttat atgtacgcat
     3541 atagtttata cctgttcttt gattttgcgg gatatagttt atttgctata ggtacagggt
     3601 acatatttgg aatacaagta cctataaact ttgacaagcc atttatcagc aaagatatga
     3661 aagagttctg gacaagatgg catataagtc tttcaagatg gtttggagat tatatttttt
     3721 ctagatttgt tatgtcatca atgagaaaga aaagatttaa gaaaaggact actgctgctc
     3781 atgtggctca gatgataacc atgataacaa tgggattttg gcatggtcta acttggtatt
     3841 atgttgctta tggtgtatac caaggattgg cacttgtgtt aactgatata tatcagcgaa
     3901 aatctaaatt ttataaaaaa cataaaaaag ataaatggtt cgagagagtg cagattttca
     3961 ttacattcca tattgtgtgt tttggattgc tgatattctc aggttattta gcttaatttt
     4021 aaggaggaaa atttatgcaa gaaacagtta tagaaatatt tgaagatgta ttaggaactg
     4081 atgagataag agaagattta gaccttaatt tatttgagac ggaactttta gactcattag
     4141 ctataattga agttttactt gaaattgaaa atagattagg gatagagctt caaccaactg
     4201 atttagagag aaaagatatg tctactgtaa ataatttagt aaaattcttg gagacaagaa
     4261 aataaattag tagttagact ttaaatatta atttataaaa taaaatttta caaagtccat
     4321 ctgatactaa tatttgatgg acttttttaa tgataataca aagtcttttg aaaataactt
     4381 taattaagta aaaaatttaa tacataaata tgatttcata aatcttaggt atcattgtaa
     4441 tattatagga gaaaaatata ttatatttag gaggaaaatg aatgtcttta aattcgatag
     4501 atagaaaaaa agaaatactt aaattattag attcaaaagg gaaggtaaac actaaagaat
     4561 tagtaaaaag attagaagta tcatcagaaa caataagaag atatttggaa gaattagaaa
     4621 atgaaaataa attaaaaaag gtatatggtg gtgctataaa aataaattat ggagtgattg
     4681 aaccagaaca tataaagaga aacaatacta acctagataa aaagcaaagt attgctaaat
     4741 atgcagctaa atttgtaaat gacaatgagt caattatcat agatgaaggt actaccaatt
     4801 tgcagattgt agattatata atatctgtaa acaatttaaa aattattaca aactcttatc
     4861 cagttctatc aaaattgatt tcttatgaga atcaaggttt gtttgatgga gaagttatat
     4921 ttattggtgg aaaagtaaat ttaaaacacc aaaggacagc atgtccaatg tcaatagata
     4981 ctatgaaaaa cttatacgta gataaagctt ttatatctag tgaaggtata cttgatagtt
     5041 ttggaattag tagtatagac caaaataagg cattgttgtc aaaagaatat ataaaaaatt
     5101 caaaggaaag ttttgtatta tgtgatagtt ctaaaatagg aattgctagt atgtataaga
     5161 tagcagattt agaggaatta gattttgtta tttgtgatgt agaacctccg aatgaatgga
     5221 aggataagtt aaatgagata aatataacat ggatagaagc aaaataaacc cttagtttta
     5281 tataatacaa ctatgctcaa tacaactaaa ttcataaaat atagtcagtt catagattaa
     5341 gattagaaag taaattttag attgtaactt aggattttat gggaatatga tacaaaataa
     5401 aaacaactaa atacaactaa aaacagaaat ttaatatata tttaacctaa atatgatgta
     5461 taattaacat atgaaagtta tactttaaaa gtaaaaacaa ttaaaaacac aaaaattcta
     5521 aaaagaatct atggaggcta taaatatgaa aaagatatat ggagaaaaaa taaaagcagt
     5581 agtattcgat tgggctggaa caacagtaga ttatggatgt tttgcacctc ttaatgtatt
     5641 tatagagata tttaaaagaa gaggtataga tgtaacaatg gaagaagcaa gaaaacctat
     5701 gggaaaatta aaaatagacc atataagaga aatgtgtgaa atggatagaa taaagaatat
     5761 ttggtcagat aaatttggta aagtgccaac tgaagatgat gtaaatgaac tttatgctga
     5821 atttgagcca atgttatttg agacattaga agattatacg actccaattc ctcatgttgt
     5881 tgaaactatt gaaaagttga gaaaaaatgg attaaaaata ggttctacaa cgggttatac
     5941 aagagagatg atgaacatag ttgaaccaaa tgcagcaaag aaaggatact cacccgattt
     6001 tcttgtaaca ccatcagaag tatctcaagg aagaccatat ccttggatgt gctataaaaa
     6061 tgctgaagct ttaggggtgt ctcctatgag ttctatggta aaagtaggag atacaataag
     6121 tgatgtaaaa gaaggcgtaa atgcaggaat gtggagtgtt gctgtaataa aaggttcaag
     6181 tgagttaggg cttacacaag aagaagtaga gaacatggat aaggaagaat taaaagctaa
     6241 gatgagtata gtttcaaaga aatttaaaga ggcaggtgca cattttgtaa tagaaactat
     6301 ggctgagtta gaagatatac taataaaaat agagaatgaa actattaaat cagattttgt
     6361 accagaaaat gattacatat tacttacacc aggaccacta tctactacta aatcagtaag
     6421 agcatctatg ttaaaagatt ggtgtacttg ggatattgaa tataataacc tagtacaaga
     6481 tgttagaaga agattagtat ctctagcaac ccaaaataca gataaataca caagtgtatt
     6541 aatgcaagga agtgggactt tttcagtaga agctataata ggaagtacta tttcaaaaga
     6601 tggtaaactt cttgtaattg caaatggagc atatggaaag agaatgaaag atatatgtaa
     6661 ttatcttaac atagaatttg ttgactgcac ttttaaagat atagaagcag tggatttaaa
     6721 tgtagtagaa aatttattaa aagaaaacaa ggatataaca catatatcaa tggttcactg
     6781 tgaaactact acaggtagat taaatcctat acaagaggta gggaaacttg caaaagaata
     6841 taacaaaatt tatatagttg atgctatgag tagttttggg ggaattgaga ttgatgttga
     6901 agattttaat atagattttt tagttagtag ttctaacaaa tgtatacaag gagtacctgg
     6961 atttggtttt ataatagcta ataaagagaa attatctaaa tgtaaaggta tagctaaatc
     7021 attatctctt gatgtgtatg cgcaatggga gactatggag aaaaataatg gtaaatggag
     7081 atttacctca cctacacatg ttgtaagagc tttttatcaa gctttattag aattggaaga
     7141 agaaggttct gttgaaaaaa gatatgctag atataaagaa aatcagttta caatagcttc
     7201 aagattaaaa tctttaggat ttgatacttt ggtgaatgat aatgctcaat cacctgtaat
     7261 aacaactttc ttatatccta aaaatgcaaa gtttgaattt atggaatttt atacttattt
     7321 aaaagataat ggatttgtta tctatccagg taaattgact gatatagata catttagaat
     7381 aggtagcata ggtgaagttt atccaactga tatggagaga ctagctgatg taatagaaaa
     7441 atttatcaat agatagaaga agatagtgtc ttgtaggtta cagaatagtg tggaaagccc
     7501 actcctttag gtgtgtgaga tgatagcaca aacagtgtat aaaaagtatt gacagtagaa
     7561 tttaaagatt tatattttag aagtttatac aatgaatata taaagattga gtaataggga
     7621 tatattttat ttaaaccaca atctaagaaa ttagatttat agtttaaata aagaatttcc
     7681 ctattatttt tatgcctatt tatagaaaat aaatgatagt tgctttatta agtattatta
     7741 tcatacttga caacgtaaca atgttttgtt atattaggtt tagagataaa atacggtaaa
     7801 gaaaggatat cattatgaat gaagaattaa gttcactaag atttgatggt gttggaaaat
     7861 gtaaaggtgg aatttttgat aatgttaata tatcaggaac aggcaaaata gaaggaagtg
     7921 taaaatgtag taaatttgat tcttctggtt cagcaaaagt ttttgggaca acagaatgta
     7981 atgaattttt tacatctggt ttaagtaaag tagaaggaaa catactggca tcaaaagttg
     8041 agatatcggg acttcttaga tgtacaggaa atattaattc tgttgaaatt gatactagtg
     8101 gaattattaa tgtagaagga agtataaaat cggatattat tcatggagaa ggatatttaa
     8161 aagcaaatga gaatatagat tgtgaaaata tcaatttaag tggaatcttg gagtgtaatg
     8221 gatttttgaa ttgtgaagaa gttaatatta gtttaaatgg aacttctaat tttaatgaag
     8281 ttggagcaag tagtataaat ataaaaaagg ggcatggaga ttattgcaaa aaatttcgta
     8341 tgctagaaaa aaagcatatc aactttgaaa aagtgtcttc aaatttaata agtttaggtc
     8401 tattatcaat tagaaagtat acagaaagta aattgtttgc aaatattata gaagcagatg
     8461 aaataagctt agaaaatagt gaagtcaaag ttgtaagagg aaaaaatatt aaaataggca
     8521 agggatgtta tgtagaaagt ttggagtaca gtgaaagtat agagattgat gaagattcaa
     8581 tagttggtga aataaagaat ataagtgaaa aaataaaatt aaagaaggat aagtaataag
     8641 taggtttaaa aataaaattt aaaatgaggt aataatatgg aaaaagggtc tataaaaata
     8701 cttggagaag gctttataag tagtggtgag tatgaaaata taaaaatcgt gggaagctct
     8761 aaatcaaaag gaaatgttaa aagtaacgaa gtaaaagtcc ttggagatgc aaaatttgaa
     8821 ggtgacatca atataaaaaa ttttgttgtt aacggaaaaa ctattataag aggcaatctt
     8881 aaagcagagc gtgtaagagt gaatggtcaa cttaatatag agggagatgc agaaatagat
     8941 gacttagtta taactgctca acagaaagtg gatggcaaac ttaaatgtaa taatgttaca
     9001 ataagaggac aattaagtgt aaatgaaaat atgtatgcac aagatataaa aatctatggc
     9061 gaaatgagaa ataaatcaaa tatagaatgt gaagatataa aggtatatgg agcaataaaa
     9121 tgtgatggtc ttttaaatgg agaaaatata tatgtttatt caggaggagg ttcttattgt
     9181 aaagagatag gggcaactaa tataaaaata ggaagaagca aagatgcttt ttcattaatt
     9241 aaaatcatta atattttttc aggaaagttt aaatgtaatt tgatagaggg agatactatt
     9301 gaattagaag atgcatcaat aaaagatata cgaggcaaaa gcatatctct agtagataat
     9361 tgtgacgttg taaatattga atacagtgat aatttatcag tttcagataa ttctactgta
     9421 aaaaacagta taaaggtggg ctagaaaagt tatggaagaa ttaatatcta agaaagattt
     9481 attgagcaaa actaatatat cttatggtca attatataga tggaaaagaa aaaacataat
     9541 accagaggaa tggtttataa aaaaatcagc ttttacaggt caagagactt tctttccaag
     9601 agataaaata ctagaaagaa tagagttaat attgtctatg aaagaggaga cttctcttga
     9661 tgatattgca aatatgttta tgaaaaagga tattaataaa gaatttgaca ttgattttat
     9721 tctcagtaaa gaagttatat gtaattatac caaagaaata tttcaaaatt tatataaaaa
     9781 agagcaaagc ataggtaaaa aagaactgct tatattgagc ataatagaga agtttttagt
     9841 taaatctata ataacttttg aagagttaaa attgatagtg aatataatag aagaaaattt
     9901 tacatcaata tacaatgaaa gtggaaagat ttatttgttt agaaagtttg gagttccatt
     9961 cgtagtagga tgtctggatt ataaaaaagt aaagtttgaa aaagatgtag ttaagattat
    10021 tgagattgac ttgacaaaag aaataaatga tataagtata aagttatcat agtgtattat
    10081 cttatataat gtattataat aatatactta aaatacactt tatttaaata tttagtttaa
    10141 agataagatt ttatgtgagt gtgtaaaagt taaagataga tttaagtagg ttatagggat
    10201 tagtaaataa taaaatgaac catgatgaat tactataaat tgtatttaaa ctacattttc
    10261 tacttatagt aatatcaatg gttcatttta ttgttattaa gataatacag tatttataaa
    10321 aatacttgat ataaatatag taaattgttt aaatagaatt attgccttaa tttattcaaa
    10381 taatgagttt ttaaaatcta agaaatatcc ttgtggatga agacatacag gacaagcttt
    10441 tggagcttct ttacctatat gtatatggcc acaatgtgta catatccatt gtatatcttc
    10501 atttcttctg aataaagtac cttcttctaa atcagtagca tatctaccaa atctatcacc
    10561 atgaactttt tctatttcag ctatattttc aaataactta gctatagatt caaatccttc
    10621 ttcacgtgct gtttttccaa agtttttata tacatcatcc cactcttgaa gctcattatg
    10681 ttgtgcagct ttaaggtgtt gtgctgtctt gtcatataaa tctacagggt atgctcctga
    10741 tatacttata ttttcaccag caaattcttt taatttagaa aagaattgtt ttgcatgtga
    10801 tttttcttgg tcagcagtgt aattaaataa agactctatt atatataatc cttctttttt
    10861 tgctacagat gcagaaatat tgtatctatt tctagcttga ctctcacctg caaaagctct
    10921 taataaattt tctttagttt tactatcttt taaattcata ttctacctct ctatcataat
    10981 aaatttttac aatatttgat tctttttatt tattattcca ataggagata taattatgct
    11041 caatttagtc agcattttta aaattatcat taattttttt taaaattcta agaaattctt
    11101 gtttttcatt ttctgtaata ttatggtatg ctgtctcttt aacttgtgtt gagatgctat
    11161 taaaagtatc ttctataagc agtgcctttt ttgtaagctt tatatacgtg atacgtttgt
    11221 catttgtaca tttttgtctt tctaaatatc ctaggtttat aagtttatta accaacactg
    11281 taacagtgga tttatctttt cctattttag ctgcaatttc tttcattgta agtattccat
    11341 tattttcata aagtgcagtt aaaatatttc catgagtagg tattaactca cctaaaccat
    11401 ttttactgag tttattttca atgaatttaa tcatactagc ctttgtttta cttatgaaat
    11461 gtacaatata tttatcttcc ataaattatt ctccaatctt taatctattt aaattagttt
    11521 gacataaaac tatttataaa ctataattag tttaacataa aactaaatgt attgggagga
    11581 aatttatgaa taaaaaattt aaatcactat atattacaga agaagaagga aattttagaa
    11641 gaaatatagt tgaaagagaa gtagatacat tgccagaagg cgaagttcta ataaaagtaa
    11701 aatattcttc tttaaattac aaggatgcac tttctgcaaa tggtaataag ggtgttacga
    11761 gaaagtatcc tcatacacca ggaattgatg catctggtat agtagaatct agtagttgtg
    11821 atgattttaa aattggagaa gaagttttag taacaggtta tgacttggga atgaatactt
    11881 ctggtggttt ttcagaatac ataagagttc cttctagttg ggtagttaaa cttcctaaaa
    11941 atctttcact aaaagaagca atggattatg gtacagcagg gtttacagca gctttatctg
    12001 tgtataaatt tatagattct gtagacaaga atatgggaga tgtattagtc actggtggta
    12061 ctggaggagt tgctgtaatt gcaactaaaa tattgattaa attaggttat tcagttgtag
    12121 tttcaacagg aaaattagaa gaacaaaaag aagtattact aaacttaggt gttaaagatg
    12181 ttatacatag gtcagaagtg gatgataact caggaagacc tctgcttaga ccaagatggg
    12241 ctggagtaat agatacagta ggaggaaata ctctagcaac agcaataaag acaactaatt
    12301 attgtggagc agtaacaact tgtggtaatg caggaggagt tgattttaca agttcagtat
    12361 atccatttat attaaaggga attactttat atggtataga ttctgtggat tgcccaatga
    12421 acttaagatt aaaaatatgg gatttattat caaatgaatg gaaattagat gatttagatg
    12481 atatggtaaa tgtagtatca ttggaagagt taaatgatag tatagattca ataatggcag
    12541 gaaaacatgt tggaagaaca gtagttgatt tagaaaaaat ataggtgaat aaatttataa
    12601 ttaggattat aggttttaag caaaaatgag atacttgaaa tttacttgtg agtatctcat
    12661 ttttttatgt aaaaagattt atgtagatgt ttgtgtaaaa agattattca tgaacaatta
    12721 attcaaatac atctggtctt gaatagtgac cttctgaatc aaaatctagt ctactaagta
    12781 ctattttttc taggtctaaa tcagctatta gcatttcttc tttattgaaa ataggacctg
    12841 ctacatattt tccaaaaggg tcaactatac aacttcctcc tggacacata atttctggtt
    12901 ctgcatctag ctctttatag tagttaaggt cagttggata catatttttt tcaacatatt
    12961 gattgcatcc aattacaaaa catcttcctt ctaaagcaat atgcttcata gttgcttgcc
    13021 actcttctct tgaatctgca gtaggggcta tgtaaagttt tacaccctta gcataaagag
    13081 ctgttctagc taatggcata taattttccc aacaaatcaa cgaacccatt tttccatatg
    13141 gagtatcaac tactgtaaga gtacttccat ctccttctcc ccaaatacat ctttctgttc
    13201 ctgtaggttt aagttttctg tgtttaccaa ttagcttacc ttctggagag aaaaataagt
    13261 ttgtgcaata aagggtacaa tttatatcat taccatctct ttctgttata cccattgata
    13321 gatatactcc tgcttcttgt gctgcttttc ctaataaatc tgttgtagca ctaggaactg
    13381 gtacagaatt atcatagtat cttttccaat cttctctacc ttccattgtt ctacttccaa
    13441 caacaaatcc aaatgaaaga cctcttggat atgcaggtat gaatgcttca gggaaaacta
    13501 cgatatttgc cccttttcta ccagcttctt taattaatcc taatgctttt tcaacagttt
    13561 tttctaaatc cattatgact ggtgacgcct ggacaacagc aattcttaca tttttatttt
    13621 ccttgctcat aaaaaacctc ctaaaaaata tttaaatttt ttgcattgaa tagattacat
    13681 gagatgttat ttgagaacta agatacttat attgtgaata ataatgtaac tgtatattta
    13741 aaataataca tcatgattta aaaaattaca attgtacaga tataattgag tttagatatt
    13801 taaatttaac aacttataat attttttcaa ttagtataaa gcctattaat ttgtcttatt
    13861 tacaatgaat agtatttact tcttggcaaa caataattaa aaataaaact tttatatgat
    13921 taaactctaa agaaaaagtt tataaagaag ataatataat taagcagaaa ataatgaaaa
    13981 aaatttatta aagtagacaa cgtaaggaga attttatgaa agaaaaaatt tattacaatg
    14041 gaaatatcat cacaatggaa gattcaatat gtggagatgc tattcttata aaagacaaaa
    14101 taataaaaaa gataggaaca aaagaagaag tatttgcttt gaaaaataaa gatacagaaa
    14161 ttatagattt acaaggtaag acattgatgc catcttttat agattcacat agtcacttga
    14221 tagcttttgc tactactcta aagctagttc ctttagaaga tgcaaccagt tttaaagaca
    14281 tagtaaaaaa aatacaggat ttcaaagagt cgaataatat aaaaaaagga gactggataa
    14341 ttggattttc atatgataat aattttttag aagaaaataa gcatccagat aagagtgtac
    14401 tcgatagtgc atcttcagaa aatccaatac taatttcaca tgcttctggt catatgggag
    14461 ttgcaaatac attggggtta aaacagcttg gtgttaccaa tgaaacacgt gacccagaag
    14521 gtggacatat aggtagagta gaaggcagtg aagaaccaaa tggatattta gaagaaaatg
    14581 cattttttaa tgtagcatct aaaataaaac aaccatcatc aaatgagatt tttaattcaa
    14641 ttgaaaaagc acagaacata tatctaagct atggtattac aacagcacaa gaaggtttaa
    14701 tggaagaaaa tcaatttaac attttaaaag caatggcaaa tcaaaataaa ttaaaaatgg
    14761 atgttgtagg atatgtaaat ttgaaaaaaa gtaagtctgt agtagataat aatagggagt
    14821 ttataaaaaa atatataaat agatttaaaa taggaggtta taagattttc ttagatggtt
    14881 ctccacaagg taagacagca tggttgagta gaccatatga aaattcagat gatggttatt
    14941 gtggatatcc tatatacaaa gatgaagaag tagaaaagtt tatagatatt tcactaaaag
    15001 aaaagatgca actattaact cattgtaatg gagatgcagc agcagaccag ttgatagatg
    15061 cgtttgagaa agttttaaac ttaaaagagc aaagtagtga aaataatata agaccagtta
    15121 tgatacatgc tcaaacagta agagcagacc agattgatga tatgaaagta ataaatatga
    15181 taccttcata ctttgtagct cacacatatt attggggtga tattcatatc aaaaaccttg
    15241 gagaagatag agcgtttaag attagcccac ttaaaacaac tatagaaaag ggattaattt
    15301 acactcttca tcaggataca ccagtaatag cacctaatat gctggaaaca gtatggtgtg
    15361 ctgtcaatag aattacaaaa aaaggtatac aaataggaga aaatgaaaag ataagcccat
    15421 tagatgcttt aaagggtgtt actataaatg cagcatatca atattttgaa gaagataaaa
    15481 aaggttcaat taaagaaggg aaattagcta atttgataat attagatgaa aacccactta
    15541 caatcgaccc tatgaaaata aaagatatta aagtattgca gactataaga gaaggagaag
    15601 tattgtattc tttgaagtag atgaaagtta gatattttat aatctataaa aaatagtttt
    15661 atataataag ttaggtagaa ttaaataaga ttacagttaa atcaacaatt atatttttat
    15721 taaattttaa ttattgtatt gaagatttat aagtataatt ttattgattc taccttttta
    15781 aataactgta gatttattaa agttatctat gtactatatt aaacaattaa gtagttatta
    15841 attgcactta ttagtttatt aaaaaactat gtatattact ttgcagtaag actttggaac
    15901 taaatctata atagcaaatt taaatttatt aaatagatta ccttataaca tttcatataa
    15961 aatatattgt gaatctgaga aaattactat ttttctttga gtattttttg taagcagttc
    16021 taaaagattc aatttcatac ctattacttg aatcattcat caaaataatt ctaatttaaa
    16081 aaataattct atc
//
LOCUS       DAKBNN010000082        16035 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000082 DAKBNN010000000
VERSION     DAKBNN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.082"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            451..1668
                     /locus_tag="OUB02_002153"
     CDS             451..1668
                     /locus_tag="OUB02_002153"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HCT8885910.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1763..2143
                     /locus_tag="OUB02_002154"
     CDS             1763..2143
                     /locus_tag="OUB02_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCT8885911.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2427..2909
                     /locus_tag="OUB02_002155"
     CDS             2427..2909
                     /locus_tag="OUB02_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8885912.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2930..3745
                     /locus_tag="OUB02_002156"
     CDS             2930..3745
                     /locus_tag="OUB02_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8885913.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3798..5348
                     /locus_tag="OUB02_002157"
     CDS             3798..5348
                     /locus_tag="OUB02_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8885914.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5445..6797
                     /locus_tag="OUB02_002158"
     CDS             5445..6797
                     /locus_tag="OUB02_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HCT8885915.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGLHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6928..7686
                     /locus_tag="OUB02_002159"
     CDS             6928..7686
                     /locus_tag="OUB02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8885916.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDNN
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7733..8452
                     /locus_tag="OUB02_002160"
     CDS             7733..8452
                     /locus_tag="OUB02_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HCT8885917.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8576..9976)
                     /locus_tag="OUB02_002161"
     CDS             complement(8576..9976)
                     /locus_tag="OUB02_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCT8885918.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9980..10888)
                     /locus_tag="OUB02_002162"
     CDS             complement(9980..10888)
                     /locus_tag="OUB02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8885919.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            11149..11796
                     /locus_tag="OUB02_002163"
     CDS             11149..11796
                     /locus_tag="OUB02_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCT8885920.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            12121..14760
                     /locus_tag="OUB02_002164"
     CDS             12121..14760
                     /locus_tag="OUB02_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCT8885921.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14818..15921)
                     /locus_tag="OUB02_002165"
     CDS             complement(14818..15921)
                     /locus_tag="OUB02_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCT8885922.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         6055 a         1907 c         2770 g         5303 t
ORIGIN      
        1 ttaattatca ccttagtttt tttattgtat tttattatat atgtatagtt ttctaatttt
       61 gaataaaaat aaagtaaaac tttctcttat attgaagttg tttagtaaga catgtatttt
      121 attaatttat taatcttaat aatgttaaat aaaatcaaaa aaatagattt tgggtaaata
      181 aaataatata aaatataaat tttaataaaa ataataaagt atatattatt gctttaataa
      241 gaatgtaaaa aatattttta aattatcatt taatgtaatt taaatgaatt gaagatatat
      301 gtttattgta tgcaattgtt gtatttttag gttttttgtg attaattaaa aaattataaa
      361 aatcgtttac atgttgattt ttaaaaaaaa aagatataga attatataga aagtaataaa
      421 caaaaattgg atttacggga gtggaatgaa atgatgaatg ttaccttaac agatgtaaaa
      481 gaggctagag agaccattaa gaacatagta aaaagaactg acttacttga aagtgttaaa
      541 cttagcgaaa aaacaggagc aaatgtgttc tataaatgtg agaatcttca aaaaacaggt
      601 tcttttaaat taagaggagc ttgtaacaaa atagcgagtt taacagatga agaaaaggca
      661 aatggagtta tagcttcaag tgcagggaat catgctcaag gagttgcatt gggagcaaaa
      721 atgactggta taaaatctac aatagtaatg ccagcaactg cgcctttagc aaaagttagt
      781 gcaactaaag gttatggagc agaagtagtt ttaaatggtg cggtttatga tgatgcatat
      841 gcaaaagctg ttgaaataca aaaagaaaca ggagcaactt tcttacatcc attcaatgat
      901 aagtatgtta tagcaggaca aggaactata tctcttgaaa ttttcgaaca attagacaat
      961 aaagttgata ctatactatg cccagttggt ggaggaggaa tcatctctgg tgttgctgtt
     1021 gcagcaaaag cattaaatcc taatgtaaaa atagtcggtg ttcaaactgc aaatatacct
     1081 tcaatgaaag agtcaataaa aaatggaaaa gtaacaacag catttaatga tactacaata
     1141 gctgatggta tagctgttaa aactccagga gatttaactt ttgaaataat aaatgaattg
     1201 gttgatgaaa tagtagtggt tgaagagaca gaaattgcag aatcaatatt atttatgatg
     1261 gaaagtcaaa aaatagtttc agaaggtgct ggtgctgtat gtacagctgc tatattaagt
     1321 ggtaagtatg taccagcaaa agatgaaaat gtagtttgta taatatctgg tggtaatata
     1381 gacataaata cgttatatag aataataggt gttgctttag ctaaagaagg aagaagatat
     1441 tcatttagta caattatgga agataagcca ggaaactttg ctgagttaac tagaataata
     1501 agtgagaatg gtggaaacat tttaagtgct aatcaaggta aattatcagc aggggaagct
     1561 ctaggaaaac aaagtgcgga atttatatta gagacaatag attatgacca tatagctaga
     1621 ataaagaaag ctatagaaga aaaaggtttt aaaattatag aattataata aaaagtgtat
     1681 ataatataca agaaaaatag tataatagtg tatgaagata cattttatgt gtattagatt
     1741 aacgaattag gaggatttta aaatgaaaca tgaagtaata catactaatg atgcaccagc
     1801 agctttagga ccatattctc aagctataaa agctggaaac ttgttatttg tatctggaca
     1861 agttcctctt gtaccagaga ctatggaagt agttgaagga gatgtacaag ctcaaactgc
     1921 tcaatcttta aaaaatctta aagctatatt agctgaaagt ggagctgatt tttcaaatgt
     1981 agttaagact acagtattta taaaagatat gaatgaattt ggggctataa acgaagttta
     2041 tgctgaatat tttggagaaa ataaaccagc aagagcttgt gtagaagttg ctagattacc
     2101 aaaagacgtt aaagttgaaa tagaagtaat agctgtatta taaaaataca atatttttaa
     2161 tttgaaaagg ctgaaaaact atattctagt gattcagcct ttttataaaa aaataaatca
     2221 caataaagaa catgataaat attgtcgaat taaaaaataa aattaaaata aatattgcaa
     2281 tatttttgag aaggttttat aatgagtata taatttgaaa cgtgtaacta ttaaattagg
     2341 catgagttct tggatatttt tatcctgagg attcatgcct tttttgtatt gaaattcaaa
     2401 aatctattta ataaggagga agtactgtgt taaatttttt caagaaaaat aaaagctata
     2461 agttacatgc agtagtgagt ggtaacagca ttaatattga gaaagtaaac gattctgtgt
     2521 tttcaaaaaa gttaatggga gatggagttg caattattcc taatagtgat gttgttgtag
     2581 ccccttgtaa tggtaaagta actgtgctta cagaatcaaa gcatgccttt ggtatggtct
     2641 cagatgaggg tgttgaaata ttagtccaca taggaattga tacagttagt ttacagggtg
     2701 aggggtttaa aaatgaagtg tctcaaggag atactgttaa aaaaggtagt ccaataataa
     2761 gttttgaaag agaaaaaata aattctcagg gaattgattg tacaacaata ataattgtat
     2821 taaatcatag cgaattttca gaaataaatt gcatggtaga aaatgaagtt gttgcagggc
     2881 aagatactgt tattgaaatt atgaaataat aggaagggat taattcaata tgagagtagt
     2941 aaaaatcttt aataataatg cattatctac agtaactaat gataggaaag aagctatttt
     3001 actaggtctt ggtataggtt ttaataaacg tccaggagat aaagttaatg aagataaaat
     3061 agaaaagatt tattatgttc aagaccacat gcaaacaaaa tttttagaat tattaaaaaa
     3121 tgttagtcca gaagtgatgg atgcttcaca acaaattata agtcttatac caggaaatga
     3181 gggaaaattt aataataaag ggatattatc tttagtggaa catattagtt ttgcaataga
     3241 tagaatgaaa aataatgttt ttttacctaa tttaatgctt ttggatataa agatgatgta
     3301 ttcaaaagag tttgaattag gagtaaaagc tttagaaatt atatatagag tatgtcatat
     3361 ccatttgcct gaagatgaag caggttacat agctttacat tttgtcaatt tacaatcaaa
     3421 tgataatttg gcatatgata ctttaaaatt tgtaaaaggt agtattgatt tgattaaaga
     3481 atgttatgga ttagaattag acgaatcgag tttaagtaca ttacgtttta gaactcatct
     3541 taaatttctt gctcaaagaa tttttaagaa tgaaatttgt caggatgata aaatgattga
     3601 aatgtatgat tatttaatta acaatcatcc taaaaataaa gaatatttag aaaagttaaa
     3661 tgtatatatt gaaaaggagt ttaggtataa attagataaa cctgaaaaaa tttatctact
     3721 actacaccta acaaaaatct tataagaatg agatgaatta gtaatagaaa tatatataat
     3781 tttagggggg gataagattg aaagaaaaac tacttggagg actggaaaca tttgcaaagg
     3841 caatggttca gccattaatg tatttatcga gtgcaggaat attaatggtg gtaggtgtac
     3901 tacttacaaa tgatatgtta ttaggagtac ttccattttt taaatgggag ccaattcaaa
     3961 tatttggaag cctaatttat aactgtataa tggctattat taataatctt gctgttatat
     4021 ttgctatagg tattcctgct gcactagcaa aaaaagagaa gcacaaagca gcgttaatag
     4081 gatttatgag ttatataata taccttacag cttccaacac tatgttaaca tcactaggtc
     4141 aactagcaaa agaaagcaag atgcttggac tgatagggac tggacaagct actattttag
     4201 gtattcaagt tgtagataca agtgtatttg gtggaattat tttagggtgt atagctggat
     4261 atgtatttaa tcgtacaagc aacaaaacat ttaaaggagc tttacagata tactcgggag
     4321 caaattttag ttttatatgc atgttctttg tatccatttt aattggagtt gtgagtaact
     4381 ttatatggcc attagctcaa caaggaatag cttctcttgc aaatgtcatc aaagatacag
     4441 gtacatttgg tttattctta tatggttttt tagagagaat attagtacca actggacttc
     4501 atcatttaat ttatacacca tttcagtttt ctgatttagg aggagtgctt aaagttggtg
     4561 cagatacagt ttcaggagcc tatgctatag ttatgacaga aatgaattta ccagttgagc
     4621 gtttctcaga tagtatatac tatatggcaa ctggatttac aaagatgttt ggttatatag
     4681 gtataggagc tgcctttatt tatactgcat ataagcaaaa taagaagaag gttaaggcaa
     4741 cgattattcc actaataatt acagcagtat tagcctctgt aacagaacca ttagacttta
     4801 tgtttgtatt ctgtgcacca cttttattct ttgtacatgc agttatagct ggtcttttta
     4861 tagctttatt aaaaatattt gatgttactg cattatgtgg agggaactta ttagtatcaa
     4921 ttataatgaa tactacagta ggcgtagaaa aaacacactg gccagtaatg atgatattag
     4981 gacttgcaca aattattgta tattttgtag tatttagttt tttaattaag aaatttaatt
     5041 ataaaactcc aggtcgtgaa gatgagagta cagctacagg cgaagaacaa tcaaaacaac
     5101 ttaatttaga tgcagggata gaaaatataa ttgatgggtt aggcggaaaa gaaaatatta
     5161 atacagtaga aaattgcatc actagattac gtgttaatgt aaaagatgaa agtaagatta
     5221 atgaagatat aattaattta acgccaaata gcgggattgt aaggaaagga aaagatatac
     5281 aaataatttt tggacttcat gtgcatgagg taagacgtgc agtagaagat tttttagaag
     5341 cttattaaag atatatattt atatagcaag ggtaaataac agctttaagt gctgtttact
     5401 atttattaaa gatacatata atttatagag gaggaataat tattatgatt attactattg
     5461 ttggtggagg aagcacattt actccaggta ttgttaagtc aattgcatta agagaaaaag
     5521 agttagagct tgaggaaatt cgtttatttg atattgataa aaaacgtcaa gataaagtag
     5581 ctgtagttgt caactggata ctaaaagaag atgttaaaac agatgttaaa ctaactgtaa
     5641 ctaattgtga aaaagaagcg tatacagatg ctacatttat atttgcacaa atgcgtgttg
     5701 gtaaatatga aatgcgtgag caggatgaaa aaattccatt gaaatatggt tgtgtaggtc
     5761 aagaaacttg tggttgtgga gggatggcat acggactgcg tacaatacaa ccaatgataa
     5821 aggttattga tgatgtagag aaatatgcta aaaaagatta ttggatatta aattactcta
     5881 atcctgctgc aatcgtttct gaagcttgtc gtaagttaag accaaatgca aggattataa
     5941 atatctgtga tatgccaata gctattatag atatgatagc ggggtcatta aatatcaatg
     6001 acgttcataa tatacgctat gattattttg gtctaaatca ttttggctgg tttacttcaa
     6061 tagattataa acatagagac ttaatggaag agattaagga atacataaaa gaaaataaaa
     6121 tattattacc aaaatcttat atagaaaact taaataaaag tcgtagtaca ggaaatcgcc
     6181 atgctgtaag tagctggata catgtttggg aatctgttta tacaatgtta gaaagcttcc
     6241 caaaatatct gccaaacact tatttaacat actatctttt aagtaaagaa acagtggaac
     6301 attcaaacat aaatcgtact cgtgcaaatg aggttatgga aagccgtgaa aaagacttat
     6361 ttgaaggtgt gagacattac ttagaaactg gagaaatttc tgaaaaagca ttttatgcag
     6421 gtttacatgg agattggatt agtgatttgg cagtttcaat taaaaatgat acacgtagtc
     6481 gttttttagt tataactgaa aatcgtggag ctataccaaa catgccatat gatgcaatgg
     6541 tagaaattcc agcatatatt ggtaaagatg gtccagaagt tattgctcga ggtccaatac
     6601 ctctatttca acaaggatta atgatgcaac aacttaacag tgaaaaattg atagtagaag
     6661 cttatataga aaatagttat gaaaaagctt tacaagcatt cacattaaat aaaactattc
     6721 caagtatgaa tgttgctaaa gaaattttgg atgaaatgat agaagttaat aaggaatact
     6781 ggccagaatt aagatagtag gataaaagaa agggttttta tttaatcatt agatatgttt
     6841 tcagagtctt ttatcaaaat gtattcacaa acatatggca atatattaaa ataaagagtg
     6901 atatttttag aagaatgggg tgatgcaatg aacattataa ataaaatttt ttggcaagaa
     6961 gataggattg gcatgattac aaataattta gaggcagata cacatagtca ctgtatgtta
     7021 caactgttct tagggataga agacagcatt gaaataacag ttaatgaaaa attagttaaa
     7081 tgtaattgta tcatagtaga taaaaatatc tctcattcat tttcagcaag aaaaaaagtt
     7141 tattactctg ctattattga acctacatct atatatgcag aacagcttac ctcaaaaatg
     7201 aatgattttg gctactggat atgtgacaat aatggattag agaaattacg tcaacaagga
     7261 acttttttga tagataactc tagtaaagaa caatatttaa gatttatgga aatgcttaat
     7321 aactatttga atatacctat aactctaaaa cattatgatg acagaattac tgaattgctc
     7381 aatttattac gtacttgtaa ttgtgataat catactattt caagttttgc agataaagtg
     7441 tctctatcag caagtagatt atcacatctg tttaaagaac aaatagggat acctttaaaa
     7501 agctatatct tatttcatca aatggagtgt gcttttagag aattactttc tggaaaaaat
     7561 gttaccgaag cttctatgac tgctggattc gatactccat cacactttgc agggacagtt
     7621 aagcgaatga tgggaatgcc tgtttcttta tctttgaaag atagcgagtt tttgaaagta
     7681 tattaaatat tctcgttata taatgaagtt gtactaagaa aggggctttg ttatgaataa
     7741 agaatattta gaagaaacaa aaatgttaaa ttataatgaa ccacaactta agcttttggt
     7801 atcatcaaag aattggttgg aattagatga ttttcataaa ataaaatcta tttatgagtt
     7861 tgtacagaat gatattttat ttggatataa tgcttctgat atgttaagcg caacacaggt
     7921 attaaatgat ggtatgggac aatgcaatac aaaagctact cttttaatgg cattattacg
     7981 tgcagtgaat atcccctgta ggcttcatgc ttttgatgta acaaaagatt ttcaacgagg
     8041 agctacatcc aaattgattt cactattagc accaaaatat attcttcata cttgggtaga
     8101 ggtattttat caagataggt ggattgctct ggagggtgtc ataactgata aaaaatattt
     8161 agaagcaatt cagaagaaat tttttaatca tggtggaacg tttaaaaagt atgcaatagc
     8221 tacaaatgat ttgaaaaata catctattga ttgggatggt aaagatactt ttatccaaaa
     8281 agaagctatc gtgtatgatt atggtatttt tccatcacca gacgtatttt tttctactca
     8341 ttcacaacat atgtcaaaat taaaaaattt catatatgta catcttatca gaaaaataat
     8401 gacaaaaaat gtatgtaagg ctagaaacaa ttatattgat aaaaatgaat gagtgtttaa
     8461 gaggtaacta tataggaaat atatagaata attaaaactg tactggttaa aatgaataaa
     8521 aaggagttat cacagagcaa acttaaattg ttcataggat aactcctttt ctgtattaac
     8581 ttctaataat ttgatgtctt tttataaaac atatagatac aattaaacca gtaaatgcaa
     8641 gagtagctgc tattgaaaat gcaacatcta ttccatgaat gttagacagc atgtttgaac
     8701 tcatatgagc tttcttagta gcacttgtca ttatagtaat aagtatagca gaacctatag
     8761 ctccagatat ctgacggagt gtgttattaa ttgctgtggc atgagatata agttctctat
     8821 ctaatgtttt aattccccat gtagttaatg gcattaaaac cattgaaata ccaatcatac
     8881 gaatacaata catcaatgac acatgaatta aagatgtatc tctgcctaaa aaagcgaagg
     8941 ataaagttcc taaaaacaaa catcctgttc ctaatattga tagagcatga ggtccatatt
     9001 tatctaaatg acgtcctgca attggattga gtacaaccat caatatagca ccaggtaaca
     9061 ttaatgaacc tgaagataat gcagaaaatc ctcttacaga ttgaacgtac agaggaatca
     9121 ttaatgtagc agacatcatt gaggcatata caatcataat taatattgta gaaataagaa
     9181 atggtttatt tttaaaaacc ctcaattcta aaaaaggttc tttagatttt aattgacgca
     9241 atgtaaataa aattaatgac ataattccta ttaatattgg taaatatgtg gcaatattag
     9301 tccacccata attaccttga ttagtaaatc caattaataa accaccaaaa cctagagatg
     9361 atagtactat tgaaggtata tctaatttta gtttttgagt ttccccaaca tttcttaata
     9421 aaataaatga tagtataaca tctaatatag caatagggga aagaaacaga aataaactat
     9481 gccaaccaaa tgaatctact aaccatcctg ataaagttgg acctattgct ggagcaaatc
     9541 ctactgtaat accaactaaa ctcatagctg caccacgttt ttcctcaggg cataaataga
     9601 gtataataac ttgtaaaagt ggcattagag aaccagaacc cattgcctgc aacacacgag
     9661 caatcaacat agttgaaaaa ttattagaaa aaagtgcaat aatacatcct atagaaaaga
     9721 tagacattga tgtaataaat aactttctag tactaaatcg atttattaaa taagctgttg
     9781 tgggtacaat aatacctgca ataagtagat aaattgttgt taaccactga ccaacatcag
     9841 cagtaatttt aaaatctgac attatattag gtaatgctgt tgacaatagt gtttgactga
     9901 gagaacttat aaaagcacca gtcattacaa ttgctattat taaatttcct tttttattgt
     9961 tactttgggt ttccataatt tataacgtct ccttttttaa ttgattttta atgctgttca
    10021 ataaaagtac ttttggtctt aaagtgtctt ctttttctaa atatgcaatc attgcatcca
    10081 taattaaaga atcatattgt tgattttttc cctcgacgta taattttttt attgcttcta
    10141 gtaatttagg attttcaagg agagaagtat ccgttttgat tagttcttct atatcttgta
    10201 tcgttttaaa aaatagcgat ttattatgaa gggaaaagta aacttcatct tcttgtttat
    10261 gtaaaaacat tgatttttca attaaaggat ttagattatc catattttct acaactaaat
    10321 ctagaatttg tatagtaaat ttggcaataa agtatacatt tatttctctt ttttgtacat
    10381 gaataatgta tataaactga tgaattagag catccaataa gaaaactaaa tcaaatataa
    10441 ttttttctat tttatcacca tagttatcaa ataagcttag tttgtattca ttcaataagt
    10501 tagctcttga ttttgtttgc aatttacttg tagccttacg ttgctcttca gaaaagtttt
    10561 ctcttatata ggtatttatg gtagttctag aaaaaatatt tttccataca gcaattatct
    10621 ttctgtttaa cttttctgat gatggaatgt cattattttc atttatggaa tgtataattt
    10681 ctaaaaactt ttcatcataa taattgatta tttcgcagat aaaactttcc ttagtttcaa
    10741 aatgcttgta aaaagtagct ttagacattt tacactcttt tacaacatcc gatattgaag
    10801 tattttgaaa acctttttgg tcaaaaattt tttgtgcaat agttataatg agcctttgtt
    10861 tttctgtcac tttattatct cctttcacgc aaaaacgttt agtactatat agtacctaca
    10921 agttttgata aatatattat acatcatttg gaaaaaaaat caatatatag actgtaatat
    10981 gaaatttttg aaaaatttgt tgttattttt agtcgtataa aaaagaaact taagagtttc
    11041 ttaagaattg tttaaagaag ttgcaataaa ttagtggtat gattatagta tgaaagagaa
    11101 tgtcttgaaa tttttctgta tcaaattcca attatggagg tgtagtatat gaaaaagaaa
    11161 atttgtgtag caataatttt tgctctattt atagtttttg tagaaatgtt tattgatagc
    11221 tctagcgaag gaagtaaaat tatgacagct gaattaagta aatacaggaa aggcgtaaat
    11281 gtattatcaa taaacaatat tatagtagcg aataaaaaat atagtttgcc caaagattat
    11341 tctcctcagg aaagcagtga agcgagagat gctttttata aaatgaataa agatgctcaa
    11401 aagtcaggat taaatttaaa agcttttagt acatatagaa gttatgaata tcaagataga
    11461 ttatttaagt catatgtaaa agaacatgga gaaaaagaag caaatagatt ttcagctaaa
    11521 cctggagaga gtgagcatca aactggactt gcttttgata ttggaggaga tgaccagtct
    11581 tgttgggcaa ataaaaagtt caacaataca aaagaagcta agtggttgta tgaaaatgca
    11641 tataaatatg gttttatact tagatatcca gaaggtaaag agaatataac aggatatatg
    11701 tatgaatctt ggcactatag atatgttgga actgaacata gcaaaaactt tgcaatgaat
    11761 aatttgactt tagaagaata tttacatata aattaagaga tggtttgtta aaattaggtt
    11821 aaaataaaaa ataacctaga ctattagctg tgtaaagtga tataatgtaa atacaattta
    11881 agataactac tcgaagggga gtagcttaca cgaaagtggt aattaatcgt caagacagta
    11941 gaaatactcg gttaattagc aagggattcc ttgatagcaa gaccttcaaa ataagcatta
    12001 ggtatgtttg ttttggggtc tttttttatt caaggaattt ttatttaaaa aaatttaaat
    12061 ttcaagtata cataagagcc cccaaacata aaataaattt attgaagagg agggcaacaa
    12121 atgtcttaca aaagtactta tcaagaagta attaaaaatt tagactcaag tttatctggt
    12181 ttgagtaaag aaagagcaga agagttgtta gaaaaaaatg gagctaatga attaaaagaa
    12241 gcagataagg ttcctactta taagcttttc ctagaaagtt ttaaagaccc attagtaata
    12301 attctattga tagctgcttt agtacaaata tttttaggtg aaacagtgga gtctataata
    12361 atttttgcag ttattattat aaactctgtt ctgggtgttg tacaaacaaa aaaagcagaa
    12421 agttcattag aaagtttgaa aaatttatct gcaccaaacg caaaggtaat aagagacaat
    12481 aaaaaaatga caattccagc aaaaaattta gtagttggag atattgtatt cttagaagct
    12541 ggcgattaca taccagcaga tggaaggctt atagaagcac aatctctcaa ggttgtagaa
    12601 gggatgttaa ctggagaatc agaaccagtt ttaaagcata cagaaaaaat aagtgaagat
    12661 gtaagtttag gtgaccagaa aaatatggta tttagtggtg ctgtagttgt ttatggtaga
    12721 ggttcttttg tagtgactgg aacaggtatg gatactgaga tgggtaaaat agctgggctt
    12781 ttagaaagtg ctgaaaataa acttacacca ttgcaacaaa aaatagagga ttttagtaaa
    12841 aaacttggtg taggtattgt aatacttgct cttttaatat ttataattca agtagcaaga
    12901 aattacttta tggctggaaa tggagaaatg acacaaatta ttttagattc attcatgttc
    12961 tctgtagcag tagcagtagc agctatacca gaagctctat catcaatagt cactatagta
    13021 ttggctgttg gaacaaatag tatggcaaaa aaacaagcca taataagaaa attaccagca
    13081 gtagaaactt taggttcaac aagtgtaata tgtactgata aaacaggtac tcttacacaa
    13141 aataaaatga cagtagttga ttattatatg tatggaacta ataatgatga gttagataaa
    13201 gaaaaagtaa actattctta tcatgctaaa ttattgacta tggtttctac attgtgtaat
    13261 gactctcata taactagtga tggtaaagaa gttggtgacc caacagaagt ggctttaata
    13321 aattattcaa gtaaaaatga tttagattat gataaattaa gaagtaagta tatcagaata
    13381 aatgaaatac catttgactc agacagaaag cttatgtcta ctgtaaatag tatatatgga
    13441 gattatataa tgtttacaaa aggtgctcca gatattgtgt ttagtagatg taaatatgct
    13501 ttgaaaaatg gttcaaaagt agatataaca gatgagataa tagaagaata taaacataaa
    13561 aatgaagagt tctcaaatag agcacttaga gtattagctt ttgcaataaa agatgtccca
    13621 gaagaagatt ttataccttc aattgatgac gaacatgaca tgacattggt tggaataatg
    13681 gcaatgatag acccaccaag agaagaagta atggatgcag taaaagaggc aaagagtgct
    13741 ggtataaaaa ctgttatgat aactggtgac cataaaacaa cagcagcagc aattgcaaaa
    13801 gagattggta taatggaaga aggagacttg gctcttactg gaaaagagct tgatgctttg
    13861 agtgaagaac aactatatga aaaactagaa aatataagtg tatatgccag agtctcgcca
    13921 gaaaataaaa ttcgtatagt aaaagcatgg caacataaga ataatataac agcaatgact
    13981 ggtgatggtg ttaatgatgc acctgcatta aaacaagcag atataggaat cggtatggga
    14041 agtggtactg atgtagcaaa agatgcttct gctatggtat tggttgatga taactttgca
    14101 agtatagtaa atgctgttga agttggtaga acagtataca gtaatataat aaaatcaatt
    14161 acttatctat ttgcaggaaa cttaggagct atagtatcca tagtatttgc agtgtttgca
    14221 ggttggtcaa atccatttac agctttacaa cttttgttta taaacttagt aaatgactct
    14281 ttaccagcaa ttgcattagg gtttgaaaag tcagaaaaaa atataatgaa taaacctcct
    14341 agacacccgg atgaaagtat actagcagga ggaacattga aggcagttgc tataagaggt
    14401 tgtataatag gtgtagttac aataattgca caatatatag gattacaagt atctccagaa
    14461 cttggagtag caatggcatt ttcaacattg atattagcta gaataatgca aacattacct
    14521 gcacgttcta ataatcaaac agtaataaaa ttaggttttt tcagcaataa atacgttata
    14581 ggagcagttt tagtctgtct tgggctttac tctttaacac taataccagt gctaagagga
    14641 atattctcta taccaacaac gtttggattg taccagttgg ggatttgcct agggttagca
    14701 ttaatttcat caatattaat ggaacttaac aaattaataa atagaagtga agtaaaataa
    14761 tcaaaaaatt aaagggcata ttgattttaa caatatgcct tttataaata gtctaattta
    14821 ttctatatta tcgattattt ggaatgcttt tataacttca ggtaattctt tttcttcagg
    14881 cattctaaat cttatgaaat ttttatcaag tccagtaaaa tcacttccag atataactct
    14941 tatttttaat ttagcaaact cttgttgtaa atctatgtcc ttgtttttat gttcaactgt
    15001 cataattgat acagtatcac tagtttcagc tatattaagg tttttccatg gaattaataa
    15061 ttgatttttt atatcttttg ttttttcttt taattcttct ataaattgaa catcatctaa
    15121 taaatttgct gcaatactac gagatatttc agacacttcg taaggagtag aaatcttttt
    15181 aatagggcta acaagttgtt ctggaagaac tgcatatcct gctcttagtc ctgctaaacc
    15241 aaatcctttt gaaaatgttt ttaaagcaat tacattatca taattattta gaagcttgac
    15301 agctgaattt tcttttggca tgtattcacc ataagcttca tctaccataa ctgctatatc
    15361 atatttagct gcttccttta ctatattttc aattgatgat aaaggtataa tttgtcctgt
    15421 aggattgttt ggattgtcta tgtaaattac tttatattct ggatttattt tttcaataaa
    15481 ttctttttca ttaaacttga aattatcctc tttctttaaa agaacataat cataagttgc
    15541 accatgcatt tttatatctg tctcatattc tgagaactga ggtgaatatc ctaaaacttt
    15601 atctcccttt tcaataaata gtctatttaa aaggtaaata acatgtatag aaccatctgc
    15661 caaacaaatt ctgtcagtat ctaatgctat aaaatctttc caataatcga ctatattatc
    15721 ttttaaagca attgaatgag gatatcctct tatcatatca aattttaaag cattaaaagc
    15781 ttctacagct ttgtttgagt aagacacggt atttatacct tcaccacagt ctatctcgaa
    15841 tacatcttct gaatgtccag attctattgc ataacttttt ttcaactgca tttcaatagc
    15901 tttgttaatt tttagtttca tactttatca ctccttattt agtcaataat aaatgttata
    15961 aaagttgcta gatttaaaat attatgaaaa tatataatag taaaaatgaa tataaatatt
    16021 ataatagctc acttt
//
LOCUS       DAKBNN010000083        15978 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000083 DAKBNN010000000
VERSION     DAKBNN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.083"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(75..2141)
                     /gene="fusA"
                     /locus_tag="OUB02_002166"
     CDS             complement(75..2141)
                     /gene="fusA"
                     /locus_tag="OUB02_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCT8885923.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
     gene            complement(2190..2660)
                     /gene="rpsG"
                     /locus_tag="OUB02_002167"
     CDS             complement(2190..2660)
                     /gene="rpsG"
                     /locus_tag="OUB02_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCT8885924.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(2784..3206)
                     /gene="rpsL"
                     /locus_tag="OUB02_002168"
     CDS             complement(2784..3206)
                     /gene="rpsL"
                     /locus_tag="OUB02_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCT8885925.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            complement(3495..6980)
                     /gene="rpoC"
                     /locus_tag="OUB02_002169"
     CDS             complement(3495..6980)
                     /gene="rpoC"
                     /locus_tag="OUB02_002169"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCT8885926.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            complement(7022..10738)
                     /gene="rpoB"
                     /locus_tag="OUB02_002170"
     CDS             complement(7022..10738)
                     /gene="rpoB"
                     /locus_tag="OUB02_002170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCT8885927.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            complement(11209..11997)
                     /locus_tag="OUB02_002171"
     CDS             complement(11209..11997)
                     /locus_tag="OUB02_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT8885928.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            complement(12388..12753)
                     /gene="rplL"
                     /locus_tag="OUB02_002172"
     CDS             complement(12388..12753)
                     /gene="rplL"
                     /locus_tag="OUB02_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCT8885929.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            complement(12811..13317)
                     /locus_tag="OUB02_002173"
     CDS             complement(12811..13317)
                     /locus_tag="OUB02_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCT8885930.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     misc_feature    complement(13360..13488)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(13540..14238)
                     /gene="rplA"
                     /locus_tag="OUB02_002174"
     CDS             complement(13540..14238)
                     /gene="rplA"
                     /locus_tag="OUB02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCT8885931.1"
                     /translation="MAKKGKRYAGALEKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            complement(14309..14734)
                     /gene="rplK"
                     /locus_tag="OUB02_002175"
     CDS             complement(14309..14734)
                     /gene="rplK"
                     /locus_tag="OUB02_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCT8885932.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            complement(14773..15315)
                     /gene="nusG"
                     /locus_tag="OUB02_002176"
     CDS             complement(14773..15315)
                     /gene="nusG"
                     /locus_tag="OUB02_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HCT8885933.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            complement(15335..15556)
                     /gene="secE"
                     /locus_tag="OUB02_002177"
     CDS             complement(15335..15556)
                     /gene="secE"
                     /locus_tag="OUB02_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCT8885934.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            complement(15582..15731)
                     /gene="rpmG"
                     /locus_tag="OUB02_002178"
     CDS             complement(15582..15731)
                     /gene="rpmG"
                     /locus_tag="OUB02_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCT8885935.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
BASE COUNT         4485 a         3249 c         1986 g         6258 t
ORIGIN      
        1 atcttgtgct agatgagaga tatctctcat ctagctgatt attttttata tttatattat
       61 taattatttt taaattattt tccttcagct attttcttag caactgaagc tggaacttgc
      121 tcatagtgat cgaatatcat tgtgtaagtt gcacgacctt gagttgaaga tcttaggtct
      181 gttgagtatc caaacatttc tgaaagtgga acgaatgcat ttataacttg tgcaccactt
      241 ctagcttcca taccttggat taatcctctt cttgagttta agtcacccat aacatctccc
      301 atgtaatctt ctggagtaac aacttctact ttgaagtaag gctctaaaag aactgcatta
      361 cctttcttca tagcatcttt gaatgccatt gaaccagcca tcttaaacgc catctcagaa
      421 gagtcaacct catggtaaga accatcatat aactcaacag caacgtctac aactggatat
      481 ccagcaacta taccagattg catagctcct tgtataccca tgtcaactgg accaacatat
      541 tcttttggta ctgatccacc aacagttttg tttgtgaatt tgtatccttc acctggctct
      601 tgaggagcaa ctctaatctt aacgtgaccg tattgtcctc taccacctga ttgcttagag
      661 tacttgtatt caacatcaac tggttgagtt atagtctctc tatatgcaac ttgtggtgca
      721 ccaactttag cttctacttt aaattctctt aataatctat ctactattat ctctaagtgt
      781 aattcaccca taccagatat tatagtttgt cctgtttctt ggtcagtctt aactgtgaaa
      841 gttgggtctt cttcagctaa tttttgaaga gctataccca ttttttcttg agctgcttta
      901 gaacttggtt ctatagcaac agatataact ggctcaggga attccataga ttcaagtatt
      961 attggattag cagggtcaca taatgtgtct ccagtagtag tatcttttaa tcctactgct
     1021 gctgctatat ctccagcata cactttagtt atttcttctc ttgtattagc atgcatttgt
     1081 aatatacgac caattctttc tctcttattc ttagttgcat ttaaaacata agaacctgat
     1141 tctaatgttc cagaataaac tctgaagaac gctagttttc caacgaatgg gtcagtcatt
     1201 attttgaatg ctaatgcaga gaatggctct tcatcagaag aatgtctttc agcttcttct
     1261 ccatctgcta atataccttt tatagctggt atatcagttg gcgctggtaa gtaatctaaa
     1321 actgcatcta ttactaactg aacacctttg tttctataag ctgtaccaca gaaaactggg
     1381 ttcatttcac aagctatagt agcttttctt atagcaactt ttaattcatc tatagtaagt
     1441 tcttcacctt caaggtattt catcattaat tcttcatcag tttcagctac tgattcaact
     1501 aatttttctc tccactcagc agctaattct ttcatatctt ctggtatatc tgtttcttcc
     1561 atttcaaccc ctaggtcgtc tttgtatata acagcatcca tttttaataa gtctattata
     1621 cctataaatg aatcttcttt acctattggt aattgcatag gaactgcatt agaatttaat
     1681 ctagatttca tcatgcttac aacattgtag aaatctgcac ctaatatatc cattttattt
     1741 acaaaagcta ttctaggtac tccataagtt tcagcttgtc tccatacatt ttctgattga
     1801 ggctcaaccc caccttttgc acagaataca gcaactgaac cgtcaagaac tcttagagat
     1861 ctttcaacct caactgtgaa gtccacgtgt ccaggagtat ctattatgtt tattctatgg
     1921 tctttccatg aagcagtagt agcggcagaa gttattgtta tacctctttc cttttcttgc
     1981 tccatccagt ccatttgaga agctccttca tgagtttctc ctattttatg agtttgccca
     2041 gtataaaaca ggattctttc tgtagtagta gtttttcctg catctatatg agccatgatt
     2101 cctatattcc tagttctttc caaaggaaac tttctagcca tggttatcct ccttatgata
     2161 tcctcaattt tataagatat ctatatatcc taccatctgt aatgagcaaa tgctttatta
     2221 gcttctgcca ttttatgagt atcttctttt ttcttaactg aagctccagt gttgttagct
     2281 gcgtccatta tctctttagc tagcttttca accattcctt tttctccacg agctctagta
     2341 tattttacta gccatcttaa acctaatgtt tgtcttcttt caggtctaac ttcgattggc
     2401 acttggtagt ttgccccacc aactcttctt gcttttactt ctaaaacagg cattatatta
     2461 tccattgctt tattaaatac ttctaaagct tcttctcctg tcttttcagc aacaattgcg
     2521 aaagcatcat aaactattct ttgagacttt cctttttttc cgtctaccat tagattattt
     2581 attaatttag taactacctt actcccatac ataggatctg gtaaaacttc tctttttgga
     2641 atattacctt ttcttggcat ttcgcttccc tccttaatta tttaacttaa atcataggta
     2701 ctcgacataa atcatttaat gccgtgatgc cgtaaatata tatatattta aagcaccgaa
     2761 ctataatcaa tcattaattg tttttacttt ttagcttctt taggtctctt cgcaccgtat
     2821 ttagatcttg attgtcttct cttatcaaca cctgctgtat ctagagtacc tcttagtata
     2881 tggtatctaa cccctggaag gtcttttact cttcctcctc ttataagaac aacactatgc
     2941 tcttgtaagt tgtgtccttc tcctggtata taagcagaaa cttctatacc attagttaat
     3001 ctaactctgg caacttttct taaagctgag ttaggcttct taggagtaac tgtttttact
     3061 gaagtacaaa cccctctttt ttgtggggca ctagcgtcag ttactttctt atttaaagag
     3121 ttgtaacctt tttgtaatgc tggagcagta gatttcttct ctagcgcttt tctactttta
     3181 cgaactaatt ggttaattgt tggcatcatc tgcacctcct tccaaatttt cgatcttatc
     3241 agtgaccgcc aactacatca atatgaatat tcatactgac tgattgtaac attcgttact
     3301 tacaatttaa tgtctataac acataaatat gtgatattag cacttttcta gcttgatgct
     3361 ttttagttct catttattgc catctaaaag tcactaagaa cttgttcaag cccttagtgt
     3421 acttttaaac aacacacttt actattttaa caccacaaat aatggttgtc aagatttctt
     3481 tactctatat gctattaatc ttcaattttt tcaacagcta tatttctata tttcttcatt
     3541 cctgttcctg ctggtattaa cttacctaat ataacatttt ctttaagtcc tattaagtgg
     3601 tcttctttac cttttattgc tgcctctgtt aatactcttg ttgtttcttg gaaagaagct
     3661 gctgataaga atgaatcagt tgcaagagat gctttagtta taccaagtaa aactctttta
     3721 gcaactgctg gtcttagacc tttatctata gcatctttat tacattcatt gaatgttaat
     3781 acatcttcat atccacctgg caataaatct gtatctcctg ggtcttcaac ttttacttta
     3841 gatagcattt gtcttactat aacttctata tgcttatcat taacatcaac cccttgaagt
     3901 ctatacactc tttgaacttc tttaactata tattcttgaa ctcccttaac accatttact
     3961 cttactatat catgaggatt tataaatcct tgtgttagag ggtctccagc ctctagcatt
     4021 tgaccttgct taacttttaa tctagaacca tatggaatta catatgtttg agtctcgcct
     4081 tcttctggta ttacatttac ttcttttctc tttcctgttt catcaatttc aactctacca
     4141 gatacttcag ttattacagc taatcctttt ggttttctag cttcaaataa ttcttcaacc
     4201 cttggtagac cttgagttat atcagctcct gcaactcctc cagtatggaa tgtacgcatt
     4261 gtaagctgag tacccggctc accgatagat tgagctgcta ttataccaac tgcttcacct
     4321 atattaactt cttttcctgt tgccaagttt ctaccataac acttagaaca aactccatga
     4381 ttagttttac agttaagaac tgttcttatc ctaatttttt caattcctaa agctactata
     4441 gtttctgctt catcttcttg tatcattgaa tcagcctcaa ctataacttc tccagtttca
     4501 ggatttaata tagggtctat agtatatctt cctacaattc tatcatatat ctcttctata
     4561 acttcatttc cttcttttat agcataaatc tctgtagtat cttctgttcc acaatctatt
     4621 tcccttacaa taacatcttg acttacatca acaagtcttc ttgttaaata tccagattca
     4681 gctgtacgta tagctgtatc ggcaagacct tttctggcac catgtgaaga tgtaaagtat
     4741 tctagtactg ataaaccttc acggaaatta gatttaactg gtatctcaac tgttttacca
     4801 gatgcatttg ccataagacc acgcatacct gccagctgtc taatttggtt tttagaacct
     4861 ctggctcctg aatgtgccat tatatatata ttatttaatc tatctagtcc acccataaga
     4921 gcatcagtaa ctttatcagt tgtttctgtc catgtctcta taactttttc atatctttct
     4981 tcatcagaga ttagacctct tctgtatgct ttttcatact tatcaacttt agcttctgcc
     5041 tcagctataa atactttctt ttcttctggg acactcatat catcaacagc aactgttatg
     5101 ccacctaatg tagaatattt aaatcctaga gcttttatat aatctagtaa ttcagctgtt
     5161 tctgtatttc cgtgttttct aaagcattta tctattattt taccaagaga ttttttatct
     5221 gctaagaaat caacttcaag tgcaaatgga ttttcttttc tatctacaaa acctaagtct
     5281 tgaggtatat tttcattaaa tataaatcta ccaacagtac tttcaaccaa tgaacttctt
     5341 ccatcttcaa gtgttacctt aagtttcact aatgcatgta gatgaactga tttattgtaa
     5401 taagcaagta atagttcatt aaagtcttta aataccatac ctgttccttt agctccatct
     5461 tgggcttcta ttgttagata atagcaacct aaaaccatat cctgagatgg agtagttata
     5521 ggtgaaccat ctttaggagc aagtatatta tttacagaaa gcattaagaa tcttgcttct
     5581 gcttgtgctt ctactgataa aggtacatga actgccattt ggtcaccatc aaagtctgca
     5641 ttataagctg tacatacaag aggatgtagt tttatagcct taccttcaac taagattggt
     5701 tcaaatgctt gtatacctaa tctatgtaga gtcggtgcac ggttaagaag aactggatga
     5761 ctttttataa catcttctaa aacatcccaa acttctggct taactttttc tactatagat
     5821 ttcgcacttt ttatattatg tgcataacct tctttaacta atttatccat aacaaatggc
     5881 ttgaataatt ctaatgccat cttctttgga agtccacatt gataaaattt aagttctggt
     5941 ccaacaacta taacagaacg tcctgagtag tcaacacgct taccaagtaa gttttgacgg
     6001 aaacgacctt gcttaccttt taacatatct gataaagatt taagtggtct atttccaggt
     6061 cctgttacag gtctacctct tctaccatta tctatcaatg catcaacagc ttcttgaagc
     6121 attctttttt catttcttac aattatatct ggagctccaa gctctaataa tcttttaagt
     6181 ctattgtttc tatttataac tcttctatat aaatcattta ggtctgaagt cgcaaatctt
     6241 ccaccatcaa gttgtaccat tggtcttaaa tctggtggta ttactggtat tgcatctaaa
     6301 atcatccatt ctggtttatt tccagacttt ttaaatgcct ctacaacttc taatcttctt
     6361 attgttctaa ctttcttctg ccctgtacta tcttttaact ctgctcttag gtctttactt
     6421 tgttgctcta aatctatatt ttgtagtaat gtctttacag cttcagcacc cattcctaca
     6481 gtaaaagtat atccatactt ttcaagagca gttctgtatt ctttttctgt aagcaattgt
     6541 ttttcgttta atccagtctc tcctggatca actaccacat atgaggcaaa gtataatatt
     6601 ttttctaacg atcttggtga catatcaagt aaaagtccca ttctacttgg tatacctttg
     6661 aagtaccaga tgtgagacat aggagcagct agctctatat gtcccattct ctctcttctt
     6721 acttttgatt tagttacttc tactccacat ctatcacaaa ctacaccttt atatctaact
     6781 cttctatatt taccacagtg gcactcccag tctttttgtg gtccaaatat tctttcacag
     6841 aaaagaccat ctttttctgg ttttaaagta cggtaattta tagtttctgg ctttttaact
     6901 tctcccctag accattgtct tattttttct ggagaagcca atgctatttt tatcgactcg
     6961 aaattgttta attcaaacaa ggagttctct cccttctatt tgagatttac attaattaaa
     7021 tttacatatc aaagtcatca ctatcaaaat ctatttcttc ttcaaataaa tcatcttcag
     7081 tgaattcttc taaattctct atatcctcat cttctgcatt ttctgcaaaa tcatcttcaa
     7141 tttcttctat tatattactt tcttcaactt ctcccatatg atttacaaca tctagctcaa
     7201 attcacctat tgtatcatct tcatccacag attctcttac ttctatttct tggtcttcat
     7261 ctgtcaatac ttttacatct aagcataaac tttgaagttc ttttataaga accttaaatg
     7321 attctgggat tcctggttca ggtatatttt cacctttaac tatagcttca tatgttctaa
     7381 cacgacctac aacgtcatca gacttaacag taagtatttc ttgaagtatg tgagcagctc
     7441 catatgcttc cagagcccaa acctccatct ctccaaatct ttgtccacca aattgtgctt
     7501 taccacctag tggctgttga gtaactaatg agtatggtcc agtacttctt gcatgtagtt
     7561 tttcatctac taagtgatgt agtttcaagt agtacatata tccaacagtt attctgttgt
     7621 caaaagactc acctgttcta ccatcatata aagttaattt accatctcta ggatacccag
     7681 cttcttcaag agcatccatt atgtcatact cattagcacc atcgaatact gatgttgcta
     7741 cataccatcc taatgtctta gcagcaagtc ctaaatgaac ttctaaaacc tgaccaatgt
     7801 tcatacgtga tggtatacct tgtgggttaa gtattatgtc tagtggtgtt ccattttcca
     7861 tgaatggcat atcttcttca ggtaatactc ttgatataac ccctttatta ccatgacgac
     7921 cagccatttt atctccaact tttatttttc tctttttagc tatatagcat cttactaatt
     7981 catttactcc tggagataaa tcatctccat tttctctagt aaatactttt acatcaacta
     8041 ttataccaga ttcaccatga ggcactttaa gtgaagtatc tctaacttct ctagcctttt
     8101 ctccaaatat tgcacgaagt aatctttctt cagcagtaag ttcagtttct ccttttggag
     8161 ttactttacc aactaatata tcacctgagt ctacttctgc acctattctt attatacctc
     8221 tgtcatctaa gttcttaatt gcactatctc caacattagg tatatctcta gttatttcct
     8281 ctggccctag ttttgtatct ctagcttcac attcatattc ttctatatga attgttgata
     8341 atctatcttc tttaactaat ctttcattta ttaatatagc atcctcgtag ttataacctt
     8401 cccaagtcat gaatgctata agacagtttc ttccaagtgc aacttctcct aaatcagttg
     8461 ctggaccatc tgctataaca tcacctttta ttatctggtc acccttattt attataggtg
     8521 tttgatttat acaagtacct gagtttgaac gtttaaactt aagtagatta tatctatctc
     8581 tgtttccatc ttctcttttt ataattattt catctgctgt tactctttct gctataccag
     8641 aatttctagc aacaacaact gctccagagt cttttgctgc tctgtattct ataccagttc
     8701 ctataattgg tgcttctctt ctaactagag gcactgcctg acgctgcatg ttcgctccca
     8761 taagggcacg gttggcatca tcattttcta agaaaggtat catagcagtg gcaacagata
     8821 caacctgctt aggagatata tccatgtaat ctactctact ttcaggaacc atttcaacag
     8881 caccatttac agttctacaa acaactctat ggtttacgaa tttaccatct tctgttaatg
     8941 gctcatttgc ctgagctctt acgaataaat cttcttcgtc agcagttaaa taatgtattt
     9001 catcagtaac tgttgatgtt tctttatcaa attttctata tggtgattct ataaatccaa
     9061 attcatttat ttttgcataa gttcctagag agtttataag accgatattt ggtccctctg
     9121 gagtctctat tggacacatt ctaccatagt gtgaatggtg aacgtcacgc acttcgaatc
     9181 cagctctttc tcttgaaaga cctccaggcc caagggctga taatcttctc ttatgcgtta
     9241 actcagatag aggatttgtt tgatccatga actgagataa ctgagaacta ccaaaaaatt
     9301 cttttattgc agctgaaact ggtcttatgt taactaacgc ttgaggcgtt atagcttcca
     9361 tatcttgaac cgtcattctt tcttttataa ctctttccat tcttgaaaga ccaattctaa
     9421 cttgattttg taacaattca cctacagatc ttattcttct gtttcctagg tgatctatat
     9481 catcaatatt tcctatatta tagaatatat tgaactcata acttattgaa gctattatat
     9541 catctaataa tatatgtttt ggtataagtt ctttaattct tgattttata gcctctttta
     9601 tttcttcttc atcactatat ccatctaaaa tttcttttaa agttggataa tgtacttttt
     9661 cttttatgtt taaatcatct atgtcaaaat caatgtgaga cttaatatcc acaaaattat
     9721 ttcctataac tctaactttt ttatcatcat ccattagtag atttactaca tttatacctg
     9781 cattttgtat ggctttagcc aaatcatagc ttattttttc tccagctttt acaaatactt
     9841 ctccagtttc tggatttatt atgtcttctg ctgaaatttt attcattatt ctataacata
     9901 aagcaagttt cttattgaat ttatatctac caacttttgc taggtcatat ctttttgggt
     9961 caaagaataa agcatttaat aaagatgatg cactttctac agtaggaggt tcacctggtc
    10021 ttaatttttt gtatatctct acaagacctt cttctactgt cttagtatta tctttttcta
    10081 atgtagcaga taatctctca tcttctccta aaaggtcaat tatctctgca tctgttccta
    10141 ttcctaaagc tctaagtaaa actgtaactg gttgttttct tgttctatca actcttacag
    10201 atattacatc attagaatct gtttcatatt ctaaccatgc tcctctatta ggaattacag
    10261 tagatgaaat caatctctta cctgttttat ctctttcttc cgcatagtaa acaccaggag
    10321 atctaactaa ttgacttact ataactcttt ctgctccatt tataacgaaa gttcctcttt
    10381 ctgtcattaa agggaagtca cccataaaca cttcttgttc ttttatctca cctgtttctt
    10441 tatttataag tcttacctta acctttagag gtgcacaata tgtagcatct ctctctttac
    10501 actcttctat atcgtacttt ggtttatcat ctagagaata gtcgacaaat tccaatataa
    10561 gattaccagt ataatcttct attggtgaaa tatcatcaaa cacttctttt aaaccttctt
    10621 ttaaaaacca ctcataggaa tctacttgaa tttctataag atttggcaca tcagctattt
    10681 ccttaatttt agaaaagctc attctagttc ttttacctat cgtgacagga tgtggcattc
    10741 acttttcacc tctcaataat taaaaataaa acaatagttc attctaacgc atctaataat
    10801 tttagcataa tgtcaagtat ttttcaatat attttttatc ttatatgtaa actatgcatt
    10861 atttttgatt ttttgtaaat tattttaagc atatattatg tattatgtac aattttccaa
    10921 tataaaatcc atatttttca tttataggtc taattttcta taaaaaattt tatacaagta
    10981 ccatcccccc tatttaatta taattccata ttaaaatgat tgtaaataat tacatttata
    11041 caaatatata caatttatta aaatatacaa aataaagacc ataaataaat ttaacttgga
    11101 attttgaacc tcaaaccttt ccaatataaa ttatatctat ggcctttata aaaatatcat
    11161 ttctactata actaatgcat aagctttcat tgcatattag taatttaatt atcctaatat
    11221 attatctgca aattgtggag taggtaaacc aaatcctcct gcaacttcaa gtaaatcccc
    11281 tgttataaat gatgagtcat cactagcata gaatagaaca gcttttgcta tatcatctgg
    11341 ttcccctata cgatttaaag gaacatgctt taaaaattct tttatgaact ctggagacat
    11401 attgtctaat gctgctttag ttgcaataag ccctggaagt acagcattac atctaacatt
    11461 gtctttagca tattgtgtag ctatgttttg agttaatgag ttgattgctg cttttgatac
    11521 acagtaagct attctagata aatcaggtaa tactgaacct atacttgata tatttactat
    11581 acttcctttt ccattcttta tcatatgagg aattgctgct ttacatggta aatacacact
    11641 ttttaaatta gtatttactg tgtcaaagaa attatctgta tctccagtta caaggtcttt
    11701 gtcaagagaa gggtttgttg aaccaaaatt gtttacaagt atatctatct taccttcttt
    11761 tttaactacc tcttctatca ttgaggtaaa ggtctcttct tcacgagcat taaagtaaac
    11821 aaacttagca caaccacctt ctgcacttat tttatttata acttcattag ctaattcttc
    11881 tgaacgagct gctaagtaca cagttgctcc attttttgct aatatctctg ctgatgctaa
    11941 tccaatacct tttgttgctg cagtaactac tgcaattttt ccttgtaatt tttccattat
    12001 taactcctcc ttgaatatgt cctttaaatc ctacaaattt cgatttttct aaatatctaa
    12061 cttattttaa aatttatatt tgttaattaa ttatttatta cattttattt ataaaacact
    12121 cgtgtatttt tttaaactaa aatgtaaaat tttacatttt agtttaatta tatactttat
    12181 atacagacat tacaacaatt gtttatattt taatttgata ataatgttaa attaaaatac
    12241 cattacacca tatatgatac ccttaattta gtactattat ataaattttt ttattttata
    12301 aaaatacttt ctttataatt ccaaatactt tctttatagt tttttataaa taaaaaaaga
    12361 tacccaatgg gtatcttttt aaagaaacta cttaacttct actttagctc cagcagcttc
    12421 taatttctct tttatttgat cagcttcttc tttagaagct ccttctttaa ctgtcttagg
    12481 agcattgtca actacttctt tagcttcttt taatcctaat cctgttattt ctctaactgc
    12541 ttttataact ccaacttttg aagaaccaac gtcagttaat actacatcaa actcagtttt
    12601 ttcttcagca gctggtccac cagcagctgc accagctacc attactggag ctgaagctga
    12661 tacaccgaat ttttcttcag cagctttaac taattcattt aattctaaaa ctttcatatt
    12721 ttctatagct tctaatattt gttctattgt catttttagc acctccgaat tttatttcaa
    12781 attaatattt ataatttttt agttaagcaa ttaagcttct tcttgacctt ctgccttttt
    12841 cgctattgca tctattaagt atgcgaagtt tgatactgga gcttttaagc taccaagtaa
    12901 tttagcgata agaacttctc ttgatggtat gttagccatt tcaacaatct tagattcatc
    12961 atagaatgca ccttccacaa tacccatctt taatttcatt tttggatgag aatccatgaa
    13021 tccttttaat attctagctg gagcaactgg atcatcatat ccaaatgcta tggcattagt
    13081 tccaactaat aattcatcat taaattgctc tataccaact tctttagctg ctcttctaac
    13141 taaagtattt ttgtatactt tgtaatctac accagcttct ctcatttgtt ttcttaactc
    13201 agttacttct tcaactgtta atcctttgta atctacaaca acagcagcag aagatttttg
    13261 taacttttca actatctcag aaacaacttc tgatttaatt tctatagctt ttctcatata
    13321 gatgtgcacc tccttcacca aataggctta gtatttaccg caacattata aaaagctttt
    13381 gttgccaaaa gtagacatca aaagctttag taatctcaat ttatctaaaa cctcggcggg
    13441 atattaagct ttcgcaccca ctatctacgg caaatctatt cttttttaca ttgacaagtg
    13501 atataataac atttttgtca acctgtgtca acagttttat tattcagctg ttcttgctgg
    13561 gtttatttta actccaggtc ccattgaaga agctactgtt atacttctta aatattgtcc
    13621 tttagcagca gctggtttag cttttattat agcatccatt aatgcagtaa agttttcagt
    13681 taatttttct ccaccaaatg atacttttcc tactggaacg tgtattatgt tagttttgtc
    13741 taatctgtat tcaactttac cagcttttat ttcatctata gctttagcta catcaaatgt
    13801 aactgttcct gatttagggt ttggcattaa acctttaggt cctaatactc tacctaatct
    13861 acctactaca cccatcatat ctggagtagc aactactata tcaaagtcaa accagttttc
    13921 accttgtatt ttttgaacta attcttcagc tcctacaaag tcagcaccag cttgttcagc
    13981 ttctttagct ttttcacctt tagcaaaaac taaaactctt tttgttttac cagttccatg
    14041 aggtaataca actgcacctc ttacttgttg gtcagcatgt cttgagtcaa cacctaattt
    14101 aatgtgtgct tctactgtct catcaaattt agctccagct atatctgaaa ctaatgttaa
    14161 tgcttctgat gcatcataaa atttagttct atctactttt tctaatgcac ctgcatatct
    14221 tttacctttt ttagccattt tattttcctc ctcgtggtta atttcggctt ctagcctccc
    14281 actttataaa aaaaagtgta tcttttaatt agtcttcgat tacaacaccc atgcttctag
    14341 cagtaccagc tatcatactc atagcagctt ctactgatgc agcatttaag tcaggcatct
    14401 ttaattcagc tatttctctt acttgagctg aagtcatttt agcaactttt ttcttgtttg
    14461 gttctccaga acctgatttt aagtttaatg cttttttaat taaaactgca gctggtggag
    14521 tttttgttat aaaactgaat gatctatctt gatatacagt tataacaact ggtattatca
    14581 ttccagcttg atctgcagtt ttagcattaa attcttttgt gaatcccatt atattaacac
    14641 catgttgtcc taatgctggt ccaactggtg gcgctggagt agcctttcct gcaggtattt
    14701 gtaattttat ttgacctata acttttttag ccattttgag cgcacctcct cagatttttc
    14761 tataatatag tattagattt tttctatacc ttcaagctct attacaaata gagtcctttt
    14821 accaaatgca tttatgcata ctttgacttc tctattttct aagtcaattt cttctatgtt
    14881 acctatttgt ccactaaatg gtccttgaga tacttttact gtatctccta tttcaaaatc
    14941 aatatcgcta tttactactt taggatctgt tgtgtctatc cccatagctt tgacttcgtc
    15001 ttcacttaat ggcactggtt ttgaaccagg tccaacgaaa cctgtaactc ccttagtatt
    15061 tcttactaca taccaagatt catcagttat tatcatttta accaaaacat aacttggata
    15121 aactttacgt tgtctagttt tttcttttcc agttttagta gtttccacta cttcttctgt
    15181 tggaacaact acttgtctta tgcagtcttc catacctctt gttttaactg ctttctcaat
    15241 tgtagcttta actttatttt catgtcccga ataggtatga actacatacc aacttgcttc
    15301 ttgtaattct gacatttaac ttacctcttt tcgtctattt tagtaataac gctagtgcac
    15361 cagataatat agtgtctata ccccatacta atatagtgca tgaaataact acagttaaaa
    15421 ctatacttgt gtttttaaac agttcctttt ttgtcggcca tgtaactctt tttaattctt
    15481 gtttagtttc ttttaagtat ccaaacaaac taaacctttt tttagttttt gcaccgtcat
    15541 ttttttgggc agccattcca tactcacatc cttataaaaa actattttgt ttctttatga
    15601 gttgtatgct ttttacagaa tctacaatat ttttgtaatt ctattctgtc tggattgttt
    15661 ttcttattct tagtagtgtt gtagtttctt tgcttacact ctgtacatgc taaagttact
    15721 ttaactctca tctgatacac ctccaacttt gtaatacaat aatatatatt gttaatatat
    15781 gtgaatataa tatctttaaa atatgtattt attaaaaatc tacagtattc aagatattat
    15841 taacttccta taatcattac cttgtataag ttatcacaaa atcctatgct gtgtcaatgt
    15901 ttttgtattt ctatgttaaa aaaagaggag cataacccct ctttttgtta ttccttttaa
    15961 tccatcagtt tagtaaag
//
LOCUS       DAKBNN010000084        15679 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000084 DAKBNN010000000
VERSION     DAKBNN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.084"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(15..875)
                     /locus_tag="OUB02_002179"
     CDS             complement(15..875)
                     /locus_tag="OUB02_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCT8885936.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1192..2520)
                     /locus_tag="OUB02_002180"
     CDS             complement(1192..2520)
                     /locus_tag="OUB02_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HCT8885937.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2668..2997)
                     /locus_tag="OUB02_002181"
     CDS             complement(2668..2997)
                     /locus_tag="OUB02_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCT8885938.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKYHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3021..4310)
                     /locus_tag="OUB02_002182"
     CDS             complement(3021..4310)
                     /locus_tag="OUB02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8885939.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4326..4652)
                     /locus_tag="OUB02_002183"
     CDS             complement(4326..4652)
                     /locus_tag="OUB02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885940.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4787..5416)
                     /locus_tag="OUB02_002184"
     CDS             complement(4787..5416)
                     /locus_tag="OUB02_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885941.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5631..8288)
                     /locus_tag="OUB02_002185"
     CDS             complement(5631..8288)
                     /locus_tag="OUB02_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885942.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8647..9591)
                     /locus_tag="OUB02_002186"
     CDS             complement(8647..9591)
                     /locus_tag="OUB02_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885943.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9584..10609)
                     /locus_tag="OUB02_002187"
     CDS             complement(9584..10609)
                     /locus_tag="OUB02_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885944.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10684..12252)
                     /locus_tag="OUB02_002188"
     CDS             complement(10684..12252)
                     /locus_tag="OUB02_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8885945.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12365..13276)
                     /locus_tag="OUB02_002189"
     CDS             complement(12365..13276)
                     /locus_tag="OUB02_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885946.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13260..14246)
                     /locus_tag="OUB02_002190"
     CDS             complement(13260..14246)
                     /locus_tag="OUB02_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885947.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14606..15007
                     /locus_tag="OUB02_002191"
     CDS             14606..15007
                     /locus_tag="OUB02_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT8885948.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15010..15408
                     /locus_tag="OUB02_002192"
     CDS             15010..15408
                     /locus_tag="OUB02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HCT8885949.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4938 a         2738 c         1724 g         6279 t
ORIGIN      
        1 tctgttacat aatattattt aaatattctt ctatatttct cttcttcttc ttcagtaaac
       61 acattaaata caacttttaa atttttttca ggattttctt ttagccatag tctaactcta
      121 cttacagcaa gattagctgc ctcttttttt ggataaccaa atacacctgt agaaattcca
      181 caaaaaacaa tattttttat ttcatcaatt tcttttattg tatttaaaca tgatttataa
      241 caatgaagca gttgcttttc ttgctcttta cttggctgac caccagatac aataggtcca
      301 acagtatgta ctacaaattt tgcaggtaaa caataacctc tagttatctt agcatctcca
      361 gtatattcta aatgcccttg tttttttatt attttgtcac aatcttctct aagtctagga
      421 cctgcgcatg aatgtatctc attatccacg catagatgta atggttgtaa acaccctaat
      481 aacttgttat ttgcagcatt tactattgca tctgctctta aattcgttat attacctctc
      541 catatggcaa tcccttcctc tacttcttct atatcattta catctactaa ctctctttct
      601 tcacattcaa tcaacaataa attatgtaac atattaagta tctctggagc aatttctttt
      661 ggtggatata cattcaaaag tcctctaagt atatctctag gcttaatttc atcatttgat
      721 aaattgtata ttttagaagg aacttctttt gaaaaatatg caattaaagt gtttatattt
      781 tttcttcttt gttcgtcagt tagaggcttg atagttttat caaaatcctc aaataaattt
      841 acatcatcag cataatctct ccacttcata gccatctctc cattcttgta ttttagactt
      901 aaaaataatt atgtatttat gtacatattt atttatttct ataaacacct acataaatcc
      961 tgttaaaagt gtaaattttt tattatgcaa cttctctggt tatttctatt gattgatagt
     1021 aaattattgt acctaaattt aatccaacta acaagtaact aaacctactt tcctaactgc
     1081 aaaatacact atgtttgcca cttgtatctc atttgtaaat aaataaagtc cctacaaata
     1141 cattaactag gctatgtact aaactgtagg aactttaaaa atatattatt cttaattaag
     1201 ttttggccaa taatttttat ttgcttctat taaatcatct agtaattctt ttgcaactgt
     1261 tgcactagga actgtcttag atagcataag tgcttgccac aatttattat aagacttctc
     1321 aatccaagca tcaactacta atttttcaac tgctaattgt tgttctatta aacctttttg
     1381 gaatgttggt attttcccta ttgataatgg ttcatacccg tcttttccaa ctatacatgg
     1441 aatttcaacc atagcatcat cttgtatatt ttctatagct ccattatttt gaactatcaa
     1501 taacatacgt tcctgtgtat taaaagctag agctgtagct aaatccacta taaactctgc
     1561 atgtattcct atcttaagcc cactgccctt agaatgtcct tgcttttcta tcaatgcaca
     1621 tgaaccaaaa acttcttttt ctcttccatc aataacttca ttggcacgag tataatttac
     1681 atcagaatga tttacaacat aatctggaaa tagatagtac tttaagtaac tacttggtat
     1741 tgtagttaaa tctgttttta ctatttcttt agtaaataaa ttagtttcaa accaactatc
     1801 tttcttgtca tttttcattc cttcttcacc atttataaaa ccatattctt ttatatgttc
     1861 tactaatttt ggtaaaagtt catttccttt tttatcttta attgaagtat accaaccaaa
     1921 gtgatttaat ccaaagtatc ttatatccat atgtttacga gatttgagtc ctgcaatcct
     1981 tgctatatta tgttccatac ctattggcat atcacaaata tttattaccc tagaattagg
     2041 tctcaacctt ctaacagctt ccgctattat tgatgctgga ttagaataat ttaacatcca
     2101 acaatttgga gaatactttt ccatataatc tatgttttca agaacacctt ctatagacct
     2161 cattccataa gctattccac ctggtccaca agtttcttga ccaactaccc catgttttag
     2221 aggtatcttt tcatctagtt ctctcatttc aagttttcca actcttatat gagctaaaca
     2281 aaaatctaca tctgtataag cctctttagg gcatgttgtt gctaaatact ctatattagg
     2341 gtccttttcc tttattagta tttcacaagc tttagctacc ttattttgtc tttcttcatc
     2401 attatcatat aatttgattg ctcttaatgg taacctatct aagttatcta gcaacattaa
     2461 tatgatttct ggggtatatg tacttcctcc tccagctatt actactgaaa atttttccat
     2521 aataatatct ccttttctat ttaaaaattt actgtattta taaatatatt tataaatact
     2581 agtactttaa agtaaaaggg agttcattta ttaaatctat taatttaata aactatagcc
     2641 aaacttcaag cactatttac aagaaattta attcttatta attcttgggt ccatcatctc
     2701 aaatatatct acaagttcag atactaaaga atgagcaaat aatgcatttg aataatggtc
     2761 ttcagagtgc acaactaaaa aatcagtccc actatcttca ctattcatag catttgatag
     2821 taattctaaa tgatactttg atgctgtttg tattttctct gaggctgaat ttaaaaggtg
     2881 ttttgcttta ttgtagtcac ctttttttat ttccttaaga gacatcatta catcactttt
     2941 agcatctcct gcttctgata ttatactaaa tactttttct tccatttcag gtaccatatc
     3001 cttctccttc taatgacttg ttaaaaatca aagctatcat catctaatgt tgtagctaat
     3061 tctgcatttt tatttaattc ttccttctcc attatcttaa agaatggata atatatcaag
     3121 gtcataagta ttatacttgc aaatactaat acgattgcct tccaatctaa tgtcatcaaa
     3181 aatgctgcaa cccctggagg taaaaaacct ggtggttctg caataggtaa tcctactaat
     3241 cctactttta atgccataaa attaagtgct ccaaccaata caggtccaaa tacaaatggt
     3301 acaaacatat atgggttcat aactattggt gttccaaata ttactggctc atttattcca
     3361 aatagtgatg gtaaaatcac aacttttccc atactattaa aacttttaca cttacttctt
     3421 gacataaggt acactaatcc caaagttatc cctgaaccag ttacttgcaa gaaaccatag
     3481 taaaatccta ttgtaaacac atattttggg tctcctcctg ctgctatcag tgttgcattt
     3541 tctgccatat atgtagctgc tattggcatc catatacttg aagttattgc tgctccatga
     3601 agcccaaaga accataataa ttgagttata aatagtatta tagttactgc aaaaacatta
     3661 tctacactgc ttatagctgg tgctaagaaa ttcattataa tagctggtag tggttgacct
     3721 gagacatttt tagttattgc tgctattgct gtaaataaca ttatacatac tatcattgga
     3781 gttaagtttc taaaactttt atttactaca ctaggtactt cttttggaaa tctaattacg
     3841 agatttttat cattcataaa cttagtaata tcaactacta aaagtgctac taatatactt
     3901 acaaataaac cttgtgcatc taaataactt gtatctaata caccatctac tacttgtcca
     3961 catacaacaa gttgtgcaac aactgctgtc atacctattt ctacatgatt aagattatat
     4021 tttttagcta gtgcctgagc tataaaaaat gctgaatata tagataataa actcatagta
     4081 aatttattag ggataagtaa tgtatccata ttttcagata ataatgtttt tgcacttcct
     4141 tcaggtaaca tattgtttaa tgccataaat aacatactta aagaaccaac aattattatt
     4201 ggggtaatac ttatcatacc ttcttttata gcttgaagat gcctttgttt ttcaatcttt
     4261 tgtgctattg gaagaagaaa tcttcttaaa ttattttgaa gtgcttccat ttttattccc
     4321 cccaattatc tatttttata taattcgatt gccgacttca taacagaagg agcatccata
     4381 cgaccataat gcttagtatc tattaaacca actgctattt tgtaattctt acaagcttca
     4441 actacatcat cttccttaaa cctaatttgt ggtgctacaa gtactacatc tgtatcatta
     4501 gcttcaatgt atgattttaa attttcaaat gatgttgcct ctatatttat agttttagtt
     4561 tttaactttt catgctcact acaaaaactt ttcatctttt ccacaagtac acctgttgaa
     4621 gctccatttg cacatactaa taaaatattc ataattaccc tcctacaata tctaacctct
     4681 tttgttttct aattttttat cagccaattc ttactctaat tcctatcagt ttttacttta
     4741 attcttatta ttttatttta attcttagta atatttactc taattcttag tactgccaat
     4801 gattaaactt gaaagccgta tctataactt cttcttgctt atatttttct aatattttta
     4861 agtatctttc tttataatca ttatctactt ctttttctgg tattactata ctagcttcat
     4921 ttaaaaagtg gaatgaatcc cttcccattt ctaatcctct atatgtatgc ttgtcaagtg
     4981 ccacgtccat aacttctgga actttaccca ttgcaaatcc ttttataatt atatttttta
     5041 ataaatcact tgatctatct ttctcacatg cacaaagata tcttattgca tgtatgaaaa
     5101 ataaaggtct gtctccatct gcatatgaat attcctttct tacttgattt aaattattta
     5161 tgattattac agctgatggg tcccccattc ctatatcttc aactgatatt gtaagtatcc
     5221 ttctccatag cttatcttct aattctggtg atgatatata tagttcataa gcaaattcac
     5281 aagcttcttc caccatattt cttcttatag ctttttgaaa tgacgatatt atttcgtctg
     5341 cactaaatcc gtttcttgta gtcattcttg cccatgggtc atttgccttt ttatctctta
     5401 atattttatc ttgcatatta tttactcctt tttatttaaa ttttaattat ttatattact
     5461 catttaataa tataagcaag tattatgcca gtttttttag acactttaaa acactgttat
     5521 ttcaatcatt tttatcatca aaacttagat actttgtcta agtatattta tatgtgtcta
     5581 atattaatat tattttagac taaaaaaaat taccaaataa gtttttatac tcattggtaa
     5641 tctttttcga ttatttctgc atattctaat attaataata tttgatagca ctctttttca
     5701 ttaatttcta tattatattt tttatctaat tgagatacta tatcttttat tttgcttagg
     5761 ctcttgtgat aatcaagtgt tttttcttta tcaagctttt caatttctct agtatttcca
     5821 atcaacccat ctaccatgaa agctaaatgc atcataagtc caattacttt atcgtatttc
     5881 ttttctatat taaatactaa aaacaattca tcagacacat attttatatc tttaaatatc
     5941 ctattataat tagagctttt taaggatgtc tttaaacctt caactacatc atcataacct
     6001 atacttatct ttataatatt ttttaattta ttgatatttt catccttatc tatatctttt
     6061 ttgctaatat agtcaattcc tctaatatta attctttgtt cattgattac tgcaacaata
     6121 ttactacttt ctttaagttt cgtaatattt aaacttaaat ctttttctgt tttatatggg
     6181 acagatataa tatctattcc atctaagtac ctactcatct tttcttctaa tatttttctt
     6241 gttttattat ctacaccttc tgaaacatgt gatgctataa taattgtttt agacagattt
     6301 tctttatttt gaatttcatt agttccaaaa tagtactttc tcgtgttagt gatagagttg
     6361 tatacgtcat ttaaaggtaa tcccatattg gccttctgaa ctgcttctaa cacagttaat
     6421 gtcgtcacca taggaattac tcttactcca cacttttttt ctttctttat aatttcatca
     6481 aaagagttta atgaacccat atctgtaaat agtaatacat ctttattgtc tatattatta
     6541 attaaatttt taaaatctac aattacttca tcatagcttt tatctaactt catatcgaaa
     6601 gaaatagcac tgcactttcc aataatatca ccaactgctt ttaccatact acttgctgta
     6661 gtttctccat gcatagcaac tataatatta atgttattca ctattttatt ttgttcatct
     6721 tgtttagctt gtactatgaa taatgtaata tatgctacag tttctataga taaaaataaa
     6781 tcaaattcat tttcaattat tccaactatt tttaatgctg ttttaaattc acttctgtta
     6841 tttcttctaa tttgattcaa atctatgttg tttgtagagc tacttttatc caatacactt
     6901 tttatataaa ttgccattga cttaaatatt gattcactta tatctatttc ttcatcttct
     6961 ttaataattt gtcttattga ttcacacatg cttttaaact taacattatt aaatagagaa
     7021 gcatctgcta tattcatttc tacagattta ttaagattag tatttactgc attaataata
     7081 tttatatcat tgtagttaat aacttcttct tctcctaatg acgtaaatac aaaatattct
     7141 attccatgtg ttataacttc atctgcttta atagacttct ctctataatc atcactcact
     7201 tcatttatag atttatcaaa atattttttc tctatataaa ctggttcttt attatctctt
     7261 ttatatctta ggtatccatt tgcaacagct aattttatat cattttgaag ttgacctaca
     7321 ttatttttac aatcataaag taatagtgat ttaattattt ctttatgtac ttttattgaa
     7381 atagaaatac tttttgattc ttccttgaaa aaatttttga ctaattccat tctttcaatc
     7441 attgtcctat ctctcaatga tggtatttct attttcattg gtattcttct attgaatgta
     7501 tctaatagtg ttgatgtcgg attttctgta gtagcacata ttattaatac cttagactct
     7561 ctttctttcg ttgcatctcc tagtctttta aatgttccct tatctataaa agtaaacaac
     7621 atttcttgac cttctgctgg tagtctatga atttcatcta aaaatagtat ccctccattt
     7681 gcaatttcca aaagaccttt tctggaagat tctgcacctg tataagctcc ttgttctacc
     7741 ccaaaaatat gtcccataag taattgtgca ttattagcat aatcagcaca attaaatgtt
     7801 acaaaaggag ctttattttt tctaattcca ctatctatag aataatcgta catatatcta
     7861 gcaatcatac tctttcctac tcctgtttct ccatagataa tagtatgaag tccatttgta
     7921 ggatagagca ttgcgacctt gcatttttct attataggtc ttaaactatg ttctttacct
     7981 attagtgttt caaaactatc tttttttatt ttgttttcta cttttgttcc tttttttatg
     8041 ccaagcaact cactttgtgt atcaagcaat tcattcttgt taaggttaag atttgcaggg
     8101 atataataca caggtcttcc ttttatcttc gtaaccatac cctcattgaa taattggttt
     8161 aaatatgtac ttatattgtt tcttgcaatt cctgtctttt ctgataattg gttagctgta
     8221 atcccttttt cattttccct atatgatgat tctaaaactt tcattacttt atctttttta
     8281 tatatcatta caagctcccc tacattatag tattttatta actaaaaccc ctatacacac
     8341 tttatttttt aataaactct tattctttaa ctaaggttat aatttatcaa actttcctta
     8401 ctttaaatta tagaaaatta ttgtagaaaa aataagatta ttgctcattt tttctacatt
     8461 actattttta attttatagt actttcttaa caacatactt tacttgtttt taattttatt
     8521 atatttgaat ttattatatt tgaaaagtag aaataaaaaa ccctattttg gagtagtttt
     8581 aaaatttctc caaaaatagg gttttagttt gtatttcaaa taaatacctg ttcaagtatt
     8641 tttatactaa acaactttta aattttgttg gtatttattt atgtctgatt tagaagaata
     8701 tacaaaatgt ccatctgaaa cctcaatcca acttaataac ttttcatttg aacaatcata
     8761 tccattagaa ttatactcta ttctatttct tgacttagca tatctagggt caggaacagg
     8821 aattgcagaa agtagtgatt ttgtatatgg atgaatagga ttattataca attcatttga
     8881 atctgccaat tctactaatt ttccattata cataactcct atacggtcac taatatgtct
     8941 caccatagat aaatcatgag ctataaataa acaagttaaa ttcttttctt gttgcaactt
     9001 tttaaataaa tttactattt gtgcttgtat agacacatcc aaagaagcta taggctcatc
     9061 agctattata aattctgggt ctactgaaag agctcgtgct atgccaatac gctgtctttg
     9121 tccacctgaa aaatcagatg gatacttatt tgcataactt ctatcaagtc ctactagctt
     9181 tagcatctca tatactttat tcattctatc tgttttattt ttacagatac cttgaatttt
     9241 tagtggctct gaaattatat ctgcaattgt catacgtgga tttaatgatg aagtagaatc
     9301 ttgaaaaata atctgcatac ttttgtttac atcttttttt ataaatttgt acgttttttt
     9361 atctgatatg caatttcctt tgtaaattac ttctccacca gtagattcat gtagtcttat
     9421 tatagtttta ccagttgtag attttcctga acccgactct cctaccaatc caaatatctc
     9481 acccttatat atatcaaaac ttatatcatc aactgctttt acagtagtac ttttgtccaa
     9541 atgaaaatat tgctttaagt tttttatttc cagtattttt tctctattca ttagaaatca
     9601 cccttccaca atttactcta actggtacat ctacttcagg tgcatctggg tgcaataacc
     9661 aagttgctgc actatgtgta tcattaattt taaacattgg tggctctttt tcataatcta
     9721 tcttaagtgc atttttattc cttattgcaa aagcatctcc ttttggtggg ttcaataaat
     9781 ttggaggcat tccaggtata ttatataaat agtcatcctg cgaatctaaa gtaggtaatg
     9841 aacccaacaa cccccaagta tatggatgtc tagggtcata aaatatatcc tctacagttc
     9901 ctatttctac aatctttcct gcatacataa ctgctattct atcagctata gttgcaacta
     9961 ctcctaaatc atgagttata aatattattg acaaacctat tttatgttgt aaatccttta
    10021 ttaaatcaat tatttgagct tgtatagtta catctaaagc agttgtaggt tcatcagcaa
    10081 tcaatacatc tggattacag gcaagtgcta tagctattac tatacgttgt ctcattccac
    10141 ctgaaaaatg atgtggaaat tgtttaaatc ttttctcagg attatcaatt ccaaccaatt
    10201 caataagctc tatggctctc ttttttgctt cacttttact tataccttga tgaatgatta
    10261 cagcttctgc aatttgcttt cctattgata tagttggatt gagagatgtc atagggtctt
    10321 gaaatatcat tgatatattc tttcctctta tatcttccat ctctttttca gacttcttaa
    10381 ccaaatcact tgatttaagt aaaacctctc catttttaat atatccattg tatgggagta
    10441 tcctcatcaa actcttacac agtacagatt tcccacaacc agattctcca actatagcca
    10501 atgtctcacc tttttttaga ttaaaagata catttcttac agcctgaact tctccttctt
    10561 ctactttaaa acttacagat agattattaa cctccaataa atgttccaaa aaccatcaac
    10621 ccctatgcta ttcaaacctt agggatatat ttttatccct aaggtttcta attaatttca
    10681 gtattattca attgtccagt cagctatgtt ccagaatata cctacaccat gatgacctaa
    10741 aactgtatca ggtgttaatc cttttatgtt tggttttcca acatatattg catctatata
    10801 agcaataaat gtataaggca tatccttagt catttctact tggaattgtt tatataactt
    10861 taatttttca tctttatctt ctgtttctct tgctttttga agtattttat ctatagtagg
    10921 attagagtaa gcactgtaat ttgcaccttt atctgttcca aatactttat atgtatggtc
    10981 atctgggtca aatggacttc cccaacctat taaatgagca tcttgattag cccaatctgt
    11041 ttcagttaca acaacagctt tagcatcaac accaatttct tttaactgtt gtgcacatat
    11101 tttagccata tcaactctaa cttggtcact ctcacctgct gttatctcga aagctaactt
    11161 agtaccttct ttttcataga ttccatcaga acctaatttc caacctagct tttcaatttc
    11221 ttgtttagct ttttctggat tatattcaaa cttttctata tcaggattat tatatggtcc
    11281 catttgtaaa ggtgaatatg ctggtacacc atgtcctaat aatacactgt caactattgc
    11341 ttttctatct attgcatagc ttaaagcatt tggtaatcct ttagcttttt tatctttaaa
    11401 gaatttagaa ttaaaattgt ataatatacc tctatagtcg gctgttttca ttatatttac
    11461 tttaaaattc ttttcgtctt tttcaaagtt agacatatct ttaggagtta tttgagctag
    11521 gtctaattct cctgatttta attgcattgc cttagcctta tcatctggaa caatcttaaa
    11581 gactacttta tctaatcctg gttcttttac aaaataatca ctattcttaa caagtgttat
    11641 actttgacct ttgtcccatt tttcaagttt aaatggtcca gtacctattg gtttttgatt
    11701 aaattcatca gtagctatat ccttaccttc caaagcatgt tttggtaata ctcctactgt
    11761 taaataatca agcattgctg tatttggagc ttttaaagta atttttatag tattatcatt
    11821 aaccacatca atttttgtta tatcttcata atttgatgct atctcagaag cattatctgg
    11881 attcattata gtttctattg taaacttaac atcatttgct gtaaatttct ctccatcatg
    11941 ccacttaaca tcatctctta aattaaaagt atatgtattt gttgcctcat caaatttcca
    12001 gtcctttgct agacaaggaa ccaccttatt attttcatca tgagctgtta atccattaaa
    12061 tattaacgaa tttatttctc catgttcata tagtgcagga tttatacttg tatagtcatt
    12121 acttccataa acaagtacct ttccatcttt aggcgtatct gccttcttat ctttgtctcc
    12181 tccactagaa caacctgcca ttagactaag tatcattact aaacttaaaa cttttaactt
    12241 ctttaatttc atgatgctcc cccttttgct ttttataaaa tatttttcaa agctttgacc
    12301 ccatttattt aaatagatat ttcaaatacc tcagtatttg cttatttagt actcaaatgc
    12361 tgatttataa attactagat tttttattat tattctttct tatataatta cctatatttg
    12421 ttatacacac caatgttgta accaaaaata tacccggaat cagtataatc caccatctat
    12481 ttgaaagtaa tgcttcctct gacaaagata acatacttcc ccaagaaaca atttctattg
    12541 gtaaaccaat tcctaaaaag ctaagtgtcg attcagtacc aatagctgcg cctatgtttg
    12601 ttacaaccat gaacattatt gatgccacaa aatttggaag taaatgttgc tttaatacat
    12661 agaagaatcc tcctcccatg ctcttagctg ctagtatgta ttcgctattt cttatttgtc
    12721 taacttctgt tctcactatc ttagatatat tcatccaact tgttatccct attactatgg
    12781 acatagatac tggatttgaa tttccaagta tagcttgtac aaatattata attaatattg
    12841 aaggtatact aagtatgatt tctgtaaacc tcatcattgc atcatctacg agttcagatg
    12901 ctgaaccact tatactacca taaacaattc ctataacagt tgatattaca gtggaaaata
    12961 gtcctataaa taaagatatt tttcctccat accatatcat agaatatata tctctaccca
    13021 ttgagtctgt tccaaataaa aaagttttat ttggagctaa attagaactt gctaaatcca
    13081 tgtatgttgg ttcatgtgtc attataaaag atgagaatac acttccaatg actatgattg
    13141 ataaaataat aattgatata tatggtaatt ctttaatttt tttagataag gttttctttg
    13201 tttctatatg ttcatcttct gctactaaaa tataattttc tcctacaatt tcaaaatcac
    13261 tatattgtgg cttcattagt atcacctcta tcatatttca ttctagggtc tattttattg
    13321 tttataattt gagctaacat attcccaaat actactaaag cccctgttat taagcataaa
    13381 actaataaca tattataatc atgatacttt gcactttcaa aacaaagtgt tcctaaccca
    13441 ggataagaaa aaactttttc aactacataa gttcctccta gtatatgagg tatcgaaatg
    13501 gccattatac taatatatga tggcattata tttctcaggc aatgcttaaa aacaatagtt
    13561 ctattattta aaccttttgc tttacataaa agcacataat cttctcttat ttcctctagt
    13621 aatttatttc ttatcatata tgtgtaatac catagatggc ttaatataag tactgtaaga
    13681 ggtaagatta gatgtgatat cctacttaat atactggact cttctcccat tgaataagca
    13741 ccactacttg gaagtatact taagtttata ctaaatataa gtatgagtac taaggcaacc
    13801 caaaatgatg gaatacagtt ggttattgtt cctaatttgc atatgattct atcaatgagc
    13861 ttatcttcat gtaatgtaca aaatattcct aacaataagg ctagcacaaa ggtaagtata
    13921 tatcctgaac cacctaatat tatagtgttt atatagacat catttataac agaagtaaca
    13981 ttttgtttat atttaaatga aattccaaat tccccttttg atgcatttga aatccatttt
    14041 atatattgac tgtatattgg ttcatccaaa ccaagttttt tcatagcatt ttctttttct
    14101 tgtgtactca ttctttctac tccatcccca tagtaagaga ttaatgggtc tcctggagca
    14161 agtcttgcca tgtaaaaaac aataaaagat aataaaaaca ttactaaaat aaactgcaac
    14221 aactttttca atatatcctt taacaaaccc caacccccct ttgttgcaat aaaaaaaacc
    14281 acataaaagc aaacgtaaag taacgttaaa accttcgtgg ttccattatc attaaacaaa
    14341 attaaatttt ctatatttat ctatgagtta attttagcat catttattaa ctgtgtaaac
    14401 aaacttttta aaaattgtta aaatttcatc aaattatttt atttatacct aaaataaaat
    14461 ttttatattc tattcttcat tattttctat aaaattataa aattttaggt tgactatata
    14521 agaaaagtat attatattag tttgtatgca aaccatttaa gaggtgattt tattgaaaag
    14581 taattatgac tgttttagaa tagccatgct cttaaaagaa ctttattcta aaactatgta
    14641 cacagtagaa gaaaatttta aagaaaatgg attaacacac caacagatta tagttataaa
    14701 actgatagct cataatcaag aacttacaat atcacaacta tgtgatgaaa tgtctttagc
    14761 taaaggtacg gtttcaggca taatcagcag attagaacag attggatata tagaaaaatt
    14821 caagaaatct aatgataaaa gaaatacata cgttaagttt acaacaacag gatttgaatt
    14881 tgccacaaat tttaaaatta aaatgcaaga aagttttgat gatattttta aaaattgtga
    14941 tgaaaacgaa ttgagtgact tagtaaaaaa tcttagaaat atattggcaa aagtaaagga
    15001 gagataatta tgaattataa attaatactt gcaatagtat ttataacatc agctttaatt
    15061 ttctacacta taggtgtatt tggagaaaga aaggcaaaaa ttttaaaaaa gaaacatgta
    15121 attatatttt ggcttggttt tatatttgat actttaggaa catttacaat gagtaacata
    15181 gccaacagcc atacttttga agtaaaatct gctttatcac aaaaccttca tagtattaca
    15241 ggtcttcttg caattgtact tatgcttttt catgcaagtt gggctacttt tgtcctatac
    15301 aaagatgatg aggaaaagaa aaaattcttt cataagttta gtatagttgt atggactata
    15361 tggttagttc catattttat aggtatgttt attggtatgg ctggatagta aaataaaaag
    15421 tgtgtaagaa atactgtagg taaatttgta taagacaaat ttatctacat ccttctttca
    15481 cacttttaca tatttattta taatactcca ttatttcttt catgtttctt aatacctata
    15541 tagaatattt aattatttct atagtatttt attattttct cacacttttt catatatata
    15601 ttgttttact attttttcat atgtatatat gaaatattcc attatttcta taaatgtttt
    15661 attatctttc tcatacttt
//
LOCUS       DAKBNN010000085        15614 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000085 DAKBNN010000000
VERSION     DAKBNN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.085"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            168..1559
                     /locus_tag="OUB02_002193"
     CDS             168..1559
                     /locus_tag="OUB02_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCT8885950.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1812..3605
                     /locus_tag="OUB02_002194"
     CDS             1812..3605
                     /locus_tag="OUB02_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT8885951.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3686..5068)
                     /locus_tag="OUB02_002195"
     CDS             complement(3686..5068)
                     /locus_tag="OUB02_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCT8885952.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISLLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5326..5673
                     /locus_tag="OUB02_002196"
     CDS             5326..5673
                     /locus_tag="OUB02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCT8885953.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5691..6725
                     /locus_tag="OUB02_002197"
     CDS             5691..6725
                     /locus_tag="OUB02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885954.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6774..7823)
                     /locus_tag="OUB02_002198"
     CDS             complement(6774..7823)
                     /locus_tag="OUB02_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8885955.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8098..9633
                     /locus_tag="OUB02_002199"
     CDS             8098..9633
                     /locus_tag="OUB02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885956.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9818..10504)
                     /locus_tag="OUB02_002200"
     CDS             complement(9818..10504)
                     /locus_tag="OUB02_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HCT8885957.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10629..12011
                     /locus_tag="OUB02_002201"
     CDS             10629..12011
                     /locus_tag="OUB02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT8885958.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIQEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12413..13681
                     /gene="eam"
                     /locus_tag="OUB02_002202"
     CDS             12413..13681
                     /gene="eam"
                     /locus_tag="OUB02_002202"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HCT8885959.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13774..14130)
                     /locus_tag="OUB02_002203"
     CDS             complement(13774..14130)
                     /locus_tag="OUB02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885960.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14281..15375)
                     /locus_tag="OUB02_002204"
     CDS             complement(14281..15375)
                     /locus_tag="OUB02_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT8885961.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         6056 a         1924 c         2504 g         5130 t
ORIGIN      
        1 aaaaaataat atttttcata attttaaaaa taataataaa aaaatgaaaa tacaggcgat
       61 aatctattga gtaaacatag aaaagatgat attataagta tgtgctaaaa tggttaaagc
      121 aatattaata ttaaatttaa ctataaaaca aaagggggcg tttttttatg tcaagtacag
      181 aaagcgtatc taaaaaaagt ggtcatccga agagtttttg gcttatttgc atgacaattt
      241 tgtgggaaag atttagttat tatggtttaa cagctttatt ggtattattt tttacagcaa
      301 gtgtagctca aggtggttta ggtctttcta ctcaagaagc aacatccatg tttggcttat
      361 ttacaggtct tatatatctt acaccattag tcggtggctg gttagcagat aaagttttag
      421 gtcaacaaaa atgtatttct ataggtgcac ttttaatagc aataggagat ttcgtactat
      481 ttgcttcatc tacatctatt gccatggttt gggtaggtct atcaataatt attttaggaa
      541 atggtttttt taaatcaagt tgctcaaata tagttgggga tatatatcct aaaactgatg
      601 tgtcaagaaa agatgcagca tacagtttat tctatatggc agtaaatgta ggagcattta
      661 tagctccaat aatatgtgga ttgctttctg ataatgtatt tgcagtaaga ggagcaagtg
      721 gagatatagt atcttatgga tataagatgg catttttaat ttgtggtata ggcatgatgt
      781 taggtatgat tatatttaca cttcttgcac caaaattatt aaatgaagtc ggaaaatatc
      841 ctgtgactaa aggtaataaa ggtgaaaaaa ttgagtatgg accacttact aagttggaga
      901 aaaataggat aatagcaatg gttattttat ttttatttgt ggtcttattc tggacagcat
      961 actatcaaac acaaagttct tttatgattt atgtaaggga taatgtaaat agaactctat
     1021 ttggatttga aatgccagta gcatggctta catcactaaa tgctatatta tgtgtggctt
     1081 tatgcccagt attagctacc ctatgggtta aattatctca taccaaaaga ggtgatttct
     1141 ctattccagt taaaatggga ttaggaatga taataatggg tgttgggttt atagttatgg
     1201 tatttgctac attagctaat ggtccagatg gaatggtaaa ggctagtata ttgtttatag
     1261 tattgactta tatatttaca acagtaggtg aattattctt atctcctata ggacttgcaa
     1321 tgttcaataa attagctcca gctaagtatg ctacgttgtc tatgggagcc tggtatctaa
     1381 caagtttttt tgcaagttta ttatcaggaa agattgctgg ttttacagcc acacttggtt
     1441 ttttacagat atttggagga atagcaacag ttttaataat atttggttta atattattgg
     1501 gaataagaaa tgtattaaca agattaatgg ctatggattt actaacaaaa gaaaaataat
     1561 tcatgacgaa ttattgtaaa taaatgaatt actttaaata aaggaagtta catttaaata
     1621 taaaattaaa tgtaatttcc tctttgatta tgaaaaaaag ctttacacaa atagatatta
     1681 taattaaaat tataattgtg ataattagag tgtataagat ttatataaat tatacaaaaa
     1741 atatttatgt aataaacttt acatacttat atatttagtt attcaaacta ttatttcgag
     1801 gaggtcttgt tgtgaatata aaagatagat taagtggatt aagaaaattt atggaagaaa
     1861 aaaatataga tgcctatatg attccatctt ctgataatca ccaaagtgaa tatgtaggag
     1921 attacttcaa atcaagagaa tttatatcag ggtttaatgg ttcagcaggg acggttattg
     1981 tgactaaaga cgaagctggg ttatggacag atggaagata ttttatacag gcagaatctc
     2041 aattagaagg aagtacaata aaattattca aaatgggaca agaaggttgc ccaactacag
     2101 atgaatattt atataaaaat attcctgaag gaggaacttt aggatttgat ggtagagtta
     2161 tatctgctag agaaggagct acattagctg aaaaattatc taaaaaaggt ataaagatag
     2221 aatatcagta tgatttaata gatagtatat ggccagatag acctgcttta tcagatagta
     2281 aagcattttt attagatgtt aaatattgtg gagaaagttt tagctcaaaa ttagctagat
     2341 taagagaaaa aatgagtgaa aaaggtactt ctactcatgt aataactact cttgatgata
     2401 tagcatggtt attcaatata agaggtggag atgttaaata taatccagtt gtactttctt
     2461 atgcagtaat aactttaaaa gaagtttatc tatttgttga tgaaagtaag ttaaatgaag
     2521 aaatactaaa tgaactagct aaggaaaatg ttcaaataaa gccatataat gatgtgtatg
     2581 aatttgtaaa aaatatagac aaaactgaaa aagttttatt agatggaact aagcttagtt
     2641 atacaattta caataatata ccttgtgaag ttgagaaagt tgatgagttc aatccagtaa
     2701 tgttttttaa agctcaaaaa aatgaggtag aattagaaaa tataagaaat tcacatgtaa
     2761 aagatggtgt agctttcact aaatttatgt actggttaaa gaaaaatgta ggtaagatgg
     2821 aaatcactga aataagtgct actcaaaaat tagaggactt aagaagagag caagaaggtt
     2881 tctttgagcc aagtttcaat acaattgctg catataaaga gcatgctgca atgatgcact
     2941 atagtgcaac tccagagtca aattataaat tagaggcaga aggattattt ttagtagatt
     3001 caggtggaca atattatgat ggaactactg atataactag gactacagtt ttaggaccta
     3061 taagtgatga attaaaacta cactttactt ctgtagcaag aggaatgata aatctttcta
     3121 aggctaagtt cttgcatggt tgtagaggat ataacttaga catactatct agaagttgca
     3181 tgtggaatat gggaattgat tatcaatgtg gaactggaca tggtatagga tttgtgctaa
     3241 atgttcacga agctccaaat ggatttagat ggagagtagt acctgaaaga tttgatagtg
     3301 cagttcttga agaaggaatg gtaactacta atgaaccagg tatatatata gaaggttctc
     3361 atggtataag aacagagaat gaaattgtag ttagaaaagc tgaaaagaat ttctatggtc
     3421 aatttatgga atttgaagtt gttacgttag ctcctattga cttagatggt atagtaccag
     3481 aattaatgaa taaggatgaa aaagattatt taaactggta tcataagtta gtttatgata
     3541 aaatatcacc tttcttaact gatgaagaaa gagaatggtt aaaagtttac actagagcta
     3601 tatagtttgt actagataca ataaaaatcg aggtattata atgcctcgat tttttattgt
     3661 atattttatt aactttaaaa aatttttaat ttgataattc tactaatttt ttattcatta
     3721 taagtagtat aattccaaat atcaataaaa atgatggtat cactatgaag acttgtaaca
     3781 taccaagttg gtctataata gcttgcgtat agccagataa tttatttgct gcaaatttgc
     3841 ctaaaaacca aacacccatt agtaatgaaa tatacttttc aggcgaaagt ttatttacca
     3901 taccataccc gactggagaa aagcaaattt ctcctatact ttggaatgtt aaaaatccaa
     3961 ctacccaaaa tacacttgct ttgtgaattg aattagggtc tgttcctgtt tgtataactg
     4021 ctactatcat aaacaaaaat gctatggcta atagtataaa acctaagctc attttttttg
     4081 gcacacttaa atcgcctttt ttagtttgag ataattttac ccatactaaa gcagagatag
     4141 gtccaagaat aacacaaagg agaccattgt atgaatctat ccatgaagta ggaatttgaa
     4201 aattgccaac agtaagattt atgtgttcct ttgtatagag tgcaatagac atagaggctt
     4261 ggttatatgc tatccaaaaa aatactgtga aaaaagctaa caccaatatt actattattc
     4321 tttttttctc aatcaaagtt aaaggtcttg aagcgagcat tttcttatat tctagtgatt
     4381 ttcttataga ctctctcccg ttaatagggt cattattcaa aggtttataa aactttctgt
     4441 ttccaaatat aaagaagaat aaacctatta gaaccgtaat tgatgaaaat aaaaatacat
     4501 atctatatcc atacatactt atagataatc cgcttttcat agcaaaccat tcattagcaa
     4561 ctaaaccgca tattaaaggg ccaaataaag tacctagatt agtagccata tacgtaatag
     4621 agaaagctcc atcttttctt gggtcatcat tggtatataa gtttccaacc atagtatgta
     4681 agtttccttt ataaaaacct attcctaatc cagataaaaa tatcatgatg tttatatcca
     4741 tcttactatt tgctatgtat cctagaaaat aaccaattga aaccattagc attccaagta
     4801 taatacagtt tttgggacct aaaaatctat cactgaccca tccaccaata actggagaga
     4861 aataagtcca tgctaaaaaa gatgcaagca ttgatgcgga ttctgctggt gttaacccta
     4921 aaccaccttt ggagacttgt tcagctaaaa acaataataa taccaatttc ataccatagt
     4981 atgcaagtcc tacaaaggta aatattgaac agcagaaata taaatctcta ggttgtttaa
     5041 gtttttcttg cgtttttaaa actgacatat gtcgagaacc tccagataaa tagtttaaca
     5101 aaattttacc atactatatg agtatatgtt tttttcatat agacgtcaac ttgtaagata
     5161 atttaattaa gtcgaaatat atgtaaaaat agtatcattt ttgtgtattc agtaatattt
     5221 ttttatgata atatatgtta ttattatatt aaaatacaaa catatttatg gaaaggatat
     5281 gccaatttag tagttagatt gattgtttgg caggtgggat gagctatgat aagaaagaaa
     5341 tctgaattac aagtagaagt aaaagaaaat ttaaatggtg gtataggaaa ggttaagctt
     5401 gaaaacataa tacaaaatga agagcttaaa ggaaaaggaa gactatttaa aagagtagct
     5461 ttaccagtag gaagttcaat tggtgtgcat gaccatacaa cagattttga agtatattat
     5521 atactaaagg gaaaaggtaa agtatttgat aatggggaat ttgtagaagt aaatgagggt
     5581 gatgtagtgt atacagctga tggcgaaaaa cattctatag aaaatatagg agaagaagat
     5641 cttgagtttg tagcactagt tttatatatg taaaatatgg aggaggagtc atgacaggca
     5701 agatagaaga aattaaggac aagataaata gattttgcaa ctccaattta aatcaagaat
     5761 acaaagatat aagctttaaa attttgcaaa accttttaga taataataaa gagataattc
     5821 attcttctag agctgatata tggtcttcag ctatactaaa tatagtttta gaacaaaatc
     5881 ttttatacaa taagaagcat ccactacata taacaaaaaa agagttttca aaacaaatag
     5941 gtgtttcttt aaatactata aataataaat cgtctttaat aaaagatgtt tgtagtattg
     6001 actttttaaa tcaagatact atagctattt ctaatgtaga attttgggta aaagagacta
     6061 attccaaatc aaagtctgta gatttagatt tagaaaagaa aaaaatacta tataaaagat
     6121 atattaagct atcacaagat gcgaatagtg atattgaatc aattagatat atggaggaag
     6181 ctgtaaatat aggtaaaagt atgattacta aagaagataa agaacttgga ttttggaaag
     6241 gtttatctac cagagcctat atgatagcac ttgaatccct tgctagaaag ttagaaagta
     6301 ttaataatct aaaggagtcc aaaaaagtat tagaatattt aatagaaatt aatcctagtg
     6361 atgagcaggg aataagatac aaattattaa atgtattaat tagattaaat gatagaactt
     6421 caataaataa attatttgaa ctttataaag aagaaaaatc tgctacatgg atgtacagta
     6481 aagctcttta ttactttaaa aataaaagta tgtttttggc aaatgattct attaaggttg
     6541 ctagaagtaa aaataagtac gttggattat atttgttaga ttggcgaaat gcttttggta
     6601 gagaatttgt tacagaggaa gaaaaggcag aagctgtcta ttactatgat gaaaacatag
     6661 tgatttggaa tgaagttaaa ggttctatgg attggctatt aaaaaaaatg ttggaatttt
     6721 cataaaaaaa actactcctt acttttatat aagtaaaagg agtagttttt aatttataca
     6781 tctagtttta aatcttcagg ttttattaga cctttttttc tcataaattc tgaaatcaac
     6841 aaagttacaa tagcaggaag tataaaatta agtaaaagta taccaaccca catagatata
     6901 cctccttttc cagcactttg catagcagtt actgttccaa attgtccaac caatccacaa
     6961 gtgcccatac cagaaccaat aggaatattc tccattttga atattgttgt agataatggt
     7021 ccaagtaata ttgaagctac agtaggtggt atccaaattt tccaattttt tattatatta
     7081 ggtacttgca acatagatgt cccaagacct tgtgccacaa ggccaccaac tccattttcc
     7141 ctaaagctca taacagcaaa gcctaccatt tggcaacaac aacctacagt tgctgcccca
     7201 ccagcaagac cacctaaact taacatcata caaatagctg cactacttat aggtaatgta
     7261 agagctattc caacaagtgc agagactaaa gctcccatta ttataggttg catagttgta
     7321 gcttgcatta ttatatttcc aaatgaagtc ataaaagcag aaacagctgg accaataagt
     7381 tcagctacaa gtactccaat tagtattgtt acagcaggtg taaccaatat atctattttt
     7441 gtttcttttg aaactaattt tccaacctct acagcaaaaa gtgtagcgac aaatgctcca
     7501 acaggtcctc ctaattcatt accacaagct ccaacaagtg cacaagaaaa tagtacaagt
     7561 ggaggtgctt gaagtccata cgcaatagcc actgctatag ctggaccagt aacctgttta
     7621 gcaagagggc ttataacttc agtaaaaaga gaaatattga aattatctcc taatgtattg
     7681 aaaattgttc caattaaaag ggacgaaaaa agaccgagtg ccatataact tagtgcgtca
     7741 ataagatacc tttgaacaga aatttcaata ttttttcttt tagcaaatga tttgaaattt
     7801 ctttttttat aattgatttc caatttaaaa tcctcctcat ataatataag tataatttct
     7861 ttttaattaa ttatataact tttcagctga caagacaaat gttatttcac taaaattaga
     7921 ctattgtaat agtaagaaat taacattaat tttagtttta aattttaaat ttatggttta
     7981 taattagtat agggatattc tgaaatataa taattttcca gtttatccca atatttaact
     8041 taggtagatt gcttacaaaa caaatgatta tatattaggc taaaaggaag tggatagatg
     8101 atatttgtaa aaaataagaa atacagaaaa attgccataa ttatatctat tcctatattg
     8161 cttgttgcgc agtactacct ttttaaattt ggaatactta gaccaatggt aaagggagta
     8221 gaaattgaaa ttgtagatgg ggaatatgtt aaagatatag ataaatatgt agtaaaatta
     8281 aatgaaacag ttactttgtc tacaggtgaa tatattaagg ttccttcgta tgctaaaaat
     8341 ccaaatattt ggtttaatgt attggataac tcagggactt taaaaataga tggaaataaa
     8401 atgacagcat taaaggttgg gtattcttct gtgggtatca tgaaaaattc aagagtatta
     8461 aaaaaggcta ctataaaagt agtagaccca gaggtagaat cactagatat ggagattaat
     8521 ggggatttaa aacatgttgg tgatagtgct actatagaaa gtacaataga agttgattat
     8581 gataagttta aaaaatcata taagccagtt tataagagta gcaatgaaaa tattttaaaa
     8641 atcaaaggaa ataaagtaag tgcagttgga gttggtaaag ctacaatata tgctaagtgt
     8701 ggaaataaag aaattgagag aacatttagg attcaagcta gagttgctga aataaatatg
     8761 gatgatataa aaatagaagt tgaccagaat gaaaaattga aaccagatat aataacttct
     8821 ccacctggtt tagaaccacc aaaaatatat tatgagttag tagattcaaa attacctgta
     8881 gaaagagcaa taagtttaag tagtaatgga aatgttgtag ggcttagaga aggtaaagat
     8941 agggtgagaa taacttgtgg agataaatct aaagtaataa cagtaacagt agtcaaagaa
     9001 actataacga atgactatat acaaaacttg gttggttcac ataaaatagt aaacaataaa
     9061 gttattatta aacttgaatg ggattatatg aaagatgtct ttgattatga tatatatctt
     9121 aaagataagt tgtcaggaga atcatttaag aaaattaaat ctataactat aaatgaaagt
     9181 gatttaggta agtctaacaa ggtatatgcg actatagaag tagatatgaa gggtaatcaa
     9241 aatatagatt ttgatattta tgtcataggg aaaaaggatt cagatacaac taagccaagt
     9301 aatgtaataa atattaccaa tggaaatgga aattcaaata atgataataa tattaaagat
     9361 atgagggtag aaaatatatc agctaatgtt gatagagata ataatgtcat tagagttaac
     9421 tggagtagta tatctcataa ggactgtact tacagtatat atgtaaaaaa taatactaat
     9481 ggagatggag aatttactct atatcaaagt gatatacagg gaaatgagca tactataagt
     9541 attcctgaag gagatttaaa tctagatatt tatgttgttg caaattataa tggaaatagc
     9601 tctcaaaata gtgatgttat aaatgtaaga tagatttgta tacaaattga aagtgttgag
     9661 tttatttaaa tattaaatta agaaaaagga tagtataggg taattatact atcctttttt
     9721 gttacaatat ataggtttaa aatctctagt aatataaatt ataaaaagtc ttatttacac
     9781 aaatctatag atattttttt tattaatcaa tattgtctta aattgaactt agtgcagctt
     9841 ctattgcatg tattactgtc gtatcaaata gtggaataga agtgtcattt tgtttaatta
     9901 aaagacctat ttctgtacat cctaatataa cagcctctgc accttgtttg ccaagcttat
     9961 ctataataga taagaaagct ttttttgaag attcagatat aattcctaag caaagttcat
    10021 taaatattgt gtcatttact attatacggt cttcttcatt tggaattaag acttcaattc
    10081 cattattaat aattacattt ttataaaaat cttgttccat tgtatattta gtacctaata
    10141 aggccacttt ttttatttct ttttccctta ataccgtagc agttacatct gctatgtgaa
    10201 gtaatggaat atgtatactt tcttgtattt tatctgatac cttatgcata gtattagtac
    10261 agataacaat aaaatctgcc cctgcctctt gtaatttaat tgcagcatca gctaaaattt
    10321 tggcgctttt ttcccaattt ccactagatt ggcattcttc aatttcttga aaatctacac
    10381 tgtaaagtat acatttagaa gagtgtagtc cacctaatct ttcttttatt actgtattaa
    10441 taacttgata atatgtaatg gtactttccc aactcatacc accaattaag cctatagtct
    10501 tcatattata cctctcttat ttatttattt tgaaatagct atttacaaat acaagtatag
    10561 catctaaaat agaagcatac aataataaaa ttgtatggag tacaattata attaagagga
    10621 gtaattatat gccaataaat tcttttgaaa actatcctat gacatggaaa ccaaaattag
    10681 ataaccgaaa accaccaata tataaaactt tagcaatgtt attagaagaa gatattaaaa
    10741 gaggaaattt aaatcctgga gataaactgc ctccacaacg ggaattagca gattttctag
    10801 atttaaactt aagtacaata acacgagcat ttaaactttg cgaagaaaag ggactaatat
    10861 gtgcaaaagt gggaaaggga acattcattt cttctgatgt aaatgtaagt aatacattat
    10921 tatatcaaac ggaatcaaaa gacataattg agttggggac agttcatcct ccgtatgagc
    10981 aaaatacata tattattgac tttattaaaa atgtactaaa acaaccagaa atggataggt
    11041 ttttacaata catgtctcca tctggcacat atatgcaaaa aaagtcaatt gcaaaatgga
    11101 tagaaagaaa taatgtatac accagtgaag aaaatatatt attatctaca ggagggcaaa
    11161 atgctatatg tgcaacttta ctaggattat ttaaagctgg tgatagaatt gctacagatt
    11221 ctttaagttt ttctggaatt aagtcaatag ctaaaatgat aggtattcaa ttagtcccaa
    11281 ttcctcaaga aaataatgaa atatcgattg aatatcttga aagttattgt aaaaatgaaa
    11341 atataaaagg catatatctt ataccagatt atcataatcc aacgacacat actatgtctg
    11401 atttatctcg taaaaagata gccaagatag caaagcaata taatataata attattgaag
    11461 atgctataaa tagcattttt agagacggta tacaaactcc catattttct ttagctagtg
    11521 ataatactat atatattttt agtacatcta aatttttatg tgctggtctt agagtagcat
    11581 ttgtagttgc tccaaaacga tatatagaaa atttagaaaa tgcactatat aatatgaatc
    11641 taatggtttc tccatttaca gcagaaatag tacaccgttt attatattct cctattatag
    11701 ataaaattat acaagagaaa agagatgcaa ttatagaaag aaatgaagtt gctgataaag
    11761 ttttgtcaga ttataatttg attggtgata agaattgcag ttttcgttgg ttacttcttc
    11821 ctgatgaatt ggatggaaaa tcatttgaaa tttctgcaaa aaatctagga gttcaagtat
    11881 attgtgctga acgattttca gtaggaaact ctacagttcc aaaagcagtt cgtatttgtg
    11941 taacagctcc aaaagatgta gaagaattag aaaaagggct taatataata aagtcacttc
    12001 ttaagagtta aatttatttt actattattt aattaaataa ataaaaaggc ggagaaattt
    12061 attataataa acctccgctt ttattattta atgaaatact ttacttttta ttaaaaaatt
    12121 taattttaat aatcttgaaa atataatcgt tttatggtaa aataattctt aaaaatagaa
    12181 aaaattaatc atttgttaaa aaaaatgcat aatttattgt aatttattga cgaaggttga
    12241 aaaatattca taaatgtgcg aaataacaga aaaaatgcaa aaattaattc aatttcattt
    12301 gttataaaat ctatatggca aaattaaatc tgcctatcag aatgatataa acacatcaga
    12361 aaggaggacg ctatcaaata atacaagtag ttaactttga tagcaaaaaa ttatgaatga
    12421 acaaactaga atatccttag agagagctgc tgaattaaaa tcaaaaattg atgattatat
    12481 tcaggctaga aaaacgatta acagaggtct tgaaaaagaa gaagagataa ataaacgaaa
    12541 acagaaaata ttaagtatct taaatggaac tgaagaggat tggaataact acaaatggca
    12601 attatcaaat agaataacag atgtagatac tttatcaaaa attataactc taactaaaaa
    12661 agaaaaagaa tatataaaag aggttggtac tcaatttaga tgggcaatat ctccatatta
    12721 tttgagtctt atagacccag aagatatatg tgacccaata aaattactgt ctataccaac
    12781 acatatagag ttggaagatg aacaagaaga tttggaccca atgggagaag agtatacaaa
    12841 cccagcagga tgtataacta gaagataccc ggatagatta ataataaatg taacaaatga
    12901 gtgtgctatg tattgtagac actgtcagag aagaagaaat attggacaac aagattctca
    12961 taagtcaaaa gctattatcc aagaatctat agactatatc agagaaaatg aagaaataag
    13021 agatgtacta gtaactggtg gagatgctct tacattaaaa gatgattatt tagagtggat
    13081 tcttagccaa cttaaagaga taccacatgt tgattatgtt agattaggta ctaggactct
    13141 tgttacaatg ccacaaagaa ttacagatga attttgcaat atgctaaaaa aatatcaccc
    13201 tatatatata aatactcatt ttaatcatcc aatggaaata actaaggaat ctaaagaagc
    13261 ttgtgaaaag ttagcaaatg caggagttcc attaggaaat caggcagtat tattaaatgg
    13321 aataaataat gataaatttg taatgagatg tttaaatcaa gaattactga aaataagagt
    13381 aaaaccttat tatatattcc aaagtaaaca tgtaaaggga acaaaacatt tcaatacatc
    13441 agtagatgat ggtcttgaaa tcatggagta tttaagagga tatacatcag gaatggctat
    13501 accaacatat atagtaaatg ctccaaaagg aggaggaaag actcctttgc ttcctcaata
    13561 ccttgtatca aaaggaacag attacgttat gcttagaaca tgggaaggaa aagttataaa
    13621 aatggaagat gaacctgctg tagatataaa gaaacttata aaagaacaag cacaggatta
    13681 aaaaaatatt taaagtttat aaaaagaaac aactttaaaa taaaaagaaa atggatgtca
    13741 aatttatatc aatttgcatc cattttttat tagttattta ttattttgtt gttgattaac
    13801 taattcaaga tatccacttg ggtggaagta atatatctca gaagtatttt catttacaaa
    13861 gtaaccaata tcaccatctg catctggtaa aaatacataa ccattaagat ttttagcttt
    13921 taatgcttca aaatcactag catttccttg atatatgtaa tcaacttcat tgttgtattt
    13981 tactaaaagt tctttagctt gttcttgagt caatttagta ttttggctta caggaacatt
    14041 cttttgagaa tctgcataag aagattcatt tactggtgca gttaataaca aacaagataa
    14101 aactaataaa gatataagtg cttttttcat actaaaaccc tctttcatgt ttaattatat
    14161 aagattcatt acttaataat attgtatgtt tcctataatt aaatattacc attaaagaga
    14221 ataatattac agttacacag ctgtaacttt tgattacaaa atcgtaatta aattgtattt
    14281 ttattccatt tgtattttat catgtctacg atttacaaac tcacagaagt atatctttat
    14341 aacgcccatc acaggagcag atagaatcat accaggtata ccaaagaatc ctccaccaac
    14401 agtaactgct aggattgtaa agaatgggct aagacctagt tgtccaccta cgatttttgg
    14461 ctctataaat gtaacttcaa cttgttgaat tattattaag taaattaaag aagttaaagc
    14521 aattgttgga ttggagaata aattaattat tacaactggt gccataccta ttactggacc
    14581 aaaatatgga accatattca tgacacccat aagtgtacca aataatagtg catattcaga
    14641 ttttaaaaag taaagaccaa ttccagaaag tactccaact ataaaagaat ctaaaatctt
    14701 acctgtaaag tatttaccaa tatttaaatt taagctttga catagttcta ttataaagtc
    14761 tccatatttt ttacctaagg aaatataaac tacttttttt gtaaacagta agaatttttc
    14821 tttttctaat aatatataga aacaaattat aaaacctaaa agaaattgta ctataaattt
    14881 tccaactgaa aatgtagtgc taaatatccc accaagtgaa ccaataagca agttactaaa
    14941 tttaggcatt acactcataa ttttgtcacc atactctttt aatgtattag ggtcaactga
    15001 ttgtaaactc ttacctaagt cgaataagaa ttgctctgtt ttatctatgt atgtaggaat
    15061 ttgagcaact aaatcagcta aactatttac tatggatgga actgtaaata atataaagga
    15121 aattagtata actatgagta caccataagt aagaagtaac gaatatattc tcttaaaatt
    15181 taatttgctt tctaagaact ttactagtgg gttgaatata tatgctaata caaatgctat
    15241 tataaatggt gttagtagtg acattaataa acttatcact ccaaaaaaat atttgtaatt
    15301 atcaataact ttaatcaaga tatatgaaac aactacagtg agtacaatat ttagataaat
    15361 ttttttatct tgcaaaataa cacaaccttt cttatattta ttaaatttag atatacttaa
    15421 atctaagtta aagttaatat ttatagctta ttatacaata aaaagcagta ttaatatagt
    15481 atgattaata tgaaattata aatacgttaa aactttatgt ttatagctaa aataataaaa
    15541 attatttata ttgtgtttat atataatcta aaatgaaaaa aagtggtgcc tttgattaaa
    15601 ctaagttttt ataa
//
LOCUS       DAKBNN010000086        15543 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000086 DAKBNN010000000
VERSION     DAKBNN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.086"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            229..3132
                     /locus_tag="OUB02_002205"
     CDS             229..3132
                     /locus_tag="OUB02_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8885962.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGTFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEGAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3165..3611
                     /locus_tag="OUB02_002206"
     CDS             3165..3611
                     /locus_tag="OUB02_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8885963.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3734..4036
                     /locus_tag="OUB02_002207"
     CDS             3734..4036
                     /locus_tag="OUB02_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8885964.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4217..5623
                     /locus_tag="OUB02_002208"
     CDS             4217..5623
                     /locus_tag="OUB02_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCT8885965.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5967..7025
                     /locus_tag="OUB02_002209"
     CDS             5967..7025
                     /locus_tag="OUB02_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT8885966.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEM"
     gene            7124..7810
                     /gene="araD"
                     /locus_tag="OUB02_002210"
     CDS             7124..7810
                     /gene="araD"
                     /locus_tag="OUB02_002210"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCT8885967.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7828..8475
                     /locus_tag="OUB02_002211"
     CDS             7828..8475
                     /locus_tag="OUB02_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCT8885968.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8648..10015)
                     /locus_tag="OUB02_002212"
     CDS             complement(8648..10015)
                     /locus_tag="OUB02_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCT8885969.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTISLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10205..10293)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10721..12841
                     /locus_tag="OUB02_002213"
     CDS             10721..12841
                     /locus_tag="OUB02_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCT8885970.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12945..14198
                     /locus_tag="OUB02_002214"
     CDS             12945..14198
                     /locus_tag="OUB02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HCT8885971.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14352..15413)
                     /gene="ytvI"
                     /locus_tag="OUB02_002215"
     CDS             complement(14352..15413)
                     /gene="ytvI"
                     /locus_tag="OUB02_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HCT8885972.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         6190 a         1758 c         2665 g         4930 t
ORIGIN      
        1 atttataaag tattttgcat taaaatttaa acaaagaatt aaattattta agattatgaa
       61 taactaatac atttagtaaa ttactaaata tacaaaatgt aaatacttat caaaatgtat
      121 aaatacttat gacatctagt tacttacagg gtttaagtag aaaaaaatat caaagtaagt
      181 atggcatgat agttgcttat atatttatca agaaaggagt tattaagcat ggagttgaga
      241 gataaacttg taaacatcat aaatacagaa aataaaaaag atccattaac agatgttcaa
      301 atagcaaagt ttttaagtac aactagagag aacataacta atttgagaaa agaacttaat
      361 ataagtaatt caaggcaaag aagatatcct tatttaaaat cagcaatttc ggcaatatta
      421 caaaagaata agaatatttc tatatcagaa attacaaggg aattgatgac agaaggcttt
      481 aatatatcta gaagagttgt ggaggaactt ttacctaaag aaagtttaga aatggatgta
      541 gtagaggaat ctgaagaaga atcgaatgac ccatttgaaa ctctgatagg taataaaggg
      601 agtcttagaa atgctgtaga gcaggctaaa tctgctatat tgtatccacc aaaagggtta
      661 ccaacgttga ttattgggga aagtggagtt ggtaagagtt tattttctag acatatgtat
      721 gagtttgcaa agcaaaaaaa gatagtaaaa gaaagctcca attttgttgt atttaactgt
      781 gctgattata gtgacaatcc acaattatta ttatctttgt tatttggata taaaaaaggt
      841 acatttacag gtgctgaata tgatacacca ggtcttgtag aagaagctga tgaaggagtt
      901 ttatttttgg atgaaataca tagattgcct ccaaaaggcc aagaaatact attttctatt
      961 ttagataggg gaaagtttag gagattaggt gaaactaatg ctgaaagaaa agtaagtatt
     1021 atgttgattg gggcaactac tgaaaatgta gagactaatt tattattaac attcagaaga
     1081 aggataccaa tgctaataac tttgccacca ttacatgata gattactaaa agagaaaata
     1141 gaccttatat acaatatctt tcaacaagag tgtaatagaa taaatgcaaa aatatttgta
     1201 gataaaaatg tgattgaaat acttgcacta aaaaagttta gtggaaatat aggacaatta
     1261 caaaatatga ttcaggttct ttgtgcaaga gcttttatga agtttataaa ttccaaagat
     1321 gaagatagtg aatcgatagt agtagtagat ataaatgaag ttttaaagct taaagattct
     1381 tttaaagatg ctgcctttca agagattgaa tatacagaga taagaaaata tttgaaaaat
     1441 gctatattta taccatttaa tttagaagaa tcatcagtaa aaggaagtct tttaagtgat
     1501 gaatatatat taccagagga catatacaaa acaattgaaa agaaatatta tgacctaaaa
     1561 tccctagaca ttgaagatat agatatagag aatattttat ggacttttat tttaaataga
     1621 tttgctgact taaagtttaa tctcgatagt aatgaagaaa ttttttcaat gagtgattta
     1681 aatagttttg taagtgatag tttagttaaa ctggtaaaag aacttatgaa tgatatagtt
     1741 gaaaaaaata tgaatttaga agtaaataaa aatatgttca aatatctagc aatacactta
     1801 gaaggagcta taaaaagaat taagctaaat caaaagataa taaatgtaaa tttagaaaaa
     1861 ataaagattg acttttcaaa agaatatgaa attagtaaaa gttttgcaat aaaaattgag
     1921 gaatcaattg gtattaagat tccagatgat gaaattggat ttataacact ctacataaag
     1981 tctgctctaa aaaatgaagt gaaaaaagat aaagtagggt taataataat ttctcatgga
     2041 cgtatagcta cagagactgt aaatgtagtt aaagaacttt taggtgtaaa gtttcctgtg
     2101 gctatagata tgcctttaga tgaaaaacca ataaatatat acaataaggc agtagaactg
     2161 tcaaaaataa tagaccaagg gaaaggcata ttgtttttgg tggatattgg ttcacttaca
     2221 aacataggtc aaatagtaaa taagagaacc aatataagca ctaaaactat tgatagagta
     2281 gacctcctaa tggcactaga agcaactaga aaagtttcta tgggagagga agaacttgat
     2341 gaaatatttt tttctttgct aaaagatagg atgggatata actatgaatt agaaaaacac
     2401 atagataaat caaatgcaat tataacatta tgtcttactg gagaaggaac tgctaagtat
     2461 ataagtaaaa cgttggaaga aaagtatgat aacactaaat gttatcaaat gagtgcattg
     2521 gatgaaaatt tattttgtaa aattgaaaat ctaaaagaga caaacaatat attagctatt
     2581 gttgggacaa taaaccctaa gataccaggt ataaatttta taccctataa taaagaactt
     2641 ttcaaaaatt tggatatcta tctatcaaaa tcaaaacaac aagatgacag accaattaaa
     2701 tcgtatgaaa gaatgcttga tgaggattta gtgatttttg aaccagatat atattttaaa
     2761 aaggatttat tagaatatgt atgttcaatt cttataaata aagaatatgt agaaaaagat
     2821 tacttagatt ctgttttaca tagagaagaa atgcttccaa catattctaa aggagctata
     2881 ggtgtgcctc atggagattc ttctactgta aatagtacaa ggtttgtttt tgttaaacta
     2941 aagaatccta ttgattgggg tgtaggtaat gtaaatttca tatttatgcc agtttttcag
     3001 gctaatgata aagagattgt taaaaatgtt cttgagatac taaaagatgc agaatttatg
     3061 aacaatgcca ataagtgttt tgataaagat gcttttagaa ggattatatt tgataaattt
     3121 aaacatctgt agccatagca aaaaaattga gaggagttag agtaatgaat gtagatttaa
     3181 tatcattggg tttagaatct aatgattatg aaagtgttat tgaagaatta ggaagtataa
     3241 tgtgtaaaaa agaatatgta aaagaaacat acataaatgc tgtcttggaa agagaaagga
     3301 cattaccaac aggtctagat attggagaaa tgtgtgttgc tattccacat acagattcaa
     3361 agcatgtaaa tgagtctaat gtagctgtag ggatattgaa aaatcctgta aagtttaatt
     3421 caatgataga cccaaaagat agacttgatg tagaacttgt atttttatta gcagtaaaga
     3481 acccagattc acaagtaaaa ctgttaaagg atttaatgtc agtttttcaa aatataaagt
     3541 tattaaaaaa tataaaaaac gcatcaacaa aagaagaagt agctaaatta ttggacttta
     3601 tagaaattta atgtaggaga attttataaa taaaaaatta aaatttaaac aatcaaagta
     3661 agtattattt ttttaatagg tttaatctta tataaaataa aaatttagag tgaaaaatta
     3721 ggaggaaata attatgaaac aatttaatgt attatcagtt tgtggttcag ggacagttac
     3781 atcatctatg gtagcaggta aattaaaaga agtactaggt aataagggga tttctattac
     3841 tacaacagaa gctagaccaa cagaagcact aaatttagca caatcaggaa gatttgactt
     3901 tataacattt acgagtccac ttcaaacagg cgattatgga ataccaacta taaatgcatt
     3961 tgcttgccta actggtatag gggaagaggc attctttgaa gaagttttga ctgttattga
     4021 agggatagat aagtaagttt tggagacgtg aatttatagt atttaaatca atgacttaga
     4081 tactataata agctaatatt aactaaatat ttgtggaaaa aacattttat atgttgtcat
     4141 aaattaaaaa attataaaaa tagtcactta agtattaaat taagcttata aatatttata
     4201 agtaggaggg aaaaagatgt tagaatttat gaaatctata atcgatacat ttggttctgc
     4261 aattatagtt ccaattataa tattcataat cgcaaagata tttaaagtaa ctactaagaa
     4321 atcgtttctt tctgctgtgt atgcaggagt tgctttacaa ggttttacac tgattttaaa
     4381 ttcatttaca ccaataataa caccagttat caataggatg gttgagagta caggagtaaa
     4441 cctaccagtt tttgatgttg gttggcaagc aacatcattg gttgcatttt caacaagtgc
     4501 aggtatgata tatttaggtc ttggtatatt attacaaaca attttatttt tgataaaatg
     4561 gacagacgtg tttcaaccat cagatttatg gaataactat tcttatatgg tatggggagc
     4621 aatggttata ggagtaacag gaaattttcc tttaggtata gcatgtatgg ttttattaaa
     4681 cctatatagt ttattaatat cagagttagt tgcaaagaga tggtctagtt actatagata
     4741 tcctaattgt acaataatag ctatgcataa tgttgaagct tctgtgtttg cagtatttgc
     4801 agaccctata tacaataaat tgggattaaa taaaataaaa ttaaatccaa aagaattaga
     4861 gaagaaatta gggtttttag gtgaaccaat tactttaggt ctttttttag gtatgtttat
     4921 aggtatatta ggtaatatga cgagaataaa tactatggaa gcttggggag aaataatgaa
     4981 agtaggtata tctacaagtg cagtgatggc aattttccct aaagtagcaa gtatgtttgc
     5041 tcaagcattt gcacctataa cagaagcagc tagaaagatt atgcaaaaag ctggaaaccg
     5101 tgagtggtac atagcagtaa atgatgctgt tggatatggt gaacctgcaa ctttaatatc
     5161 aggtcttata ttaattccaa taatgcttgt aatagctatg gtacttcctg gtaataaggt
     5221 attaccagtt gtggatttat tggcaattcc atatatggtt caaggtttag ttgccataca
     5281 taatggtaat ataccaaaag tattagtttc aggtataatt tggtttggat taggattata
     5341 tgtttgtaca tctacagcac cattatttac agatatggct acaaatatag gtgtagctat
     5401 acctgcagga gcaatgttaa taacaagctt taatatacta ggaaaacctt tgatgggatt
     5461 agttttcttt gcatttctaa gtgcaaatcc aatttatata ggtctttcag ttgtaattta
     5521 ctttgttcta tgggcattgt ttagaaagaa caaaacaagt attcttgatt atttagaaaa
     5581 acaagcactc aaaaatgtag aagaagagcc tgtagcagtt taagtttata aaaatgaaat
     5641 aaaaataatt aaaggagttt ataattgatt ttatataagt atcagataat acctgagaaa
     5701 tatttaaatg aaatatagac ttctttttat ttgataatat ttgtaattat aatgcattgt
     5761 atttttatag ttgagttttc tgtataacct gctatttaaa acatactctt ttatatttat
     5821 acagtaatgt ggtttaattt tataaaatgt aatctctact ggtaaaagac tttgtatata
     5881 aggcatttgc ctgaataaac catttatctt ttgtatggca tagatattgc tatatagaag
     5941 tatatagtat gaaaggagat atttatatgg gagataaaat gagagcatca gtattgtata
     6001 atgtaggtga tgtcagatat gaaatggtag atataccaga aataacagat acgcaagtgc
     6061 ttgtaaatgt aaaatatgtt gggatatgtg gttcagattt acctaggtct atggtaagtg
     6121 gtttgagtgg aaatacaaaa tatccattga ttttagggca tgaattttct ggtgaagttg
     6181 ttaaaattgg agaaaaagta aagcatatta atgtaggaga tagagtagct gttgcaccat
     6241 tagtaccttg tggaaaatgt gattattgta atgaaggtaa ttttggcttg tgtgatgatt
     6301 acaatataat tggaacaaga gtaaatggag cgtttgcaga atatgtaaga gttccagaag
     6361 aacatatttt aaaactacca gatacattgg attatgaaac tgctgccgga attgaaccag
     6421 caactatagc ttatcatggg atttcaaagt ctaatattag agtaggagat agtgtagttg
     6481 tgttaggatg tggtccaata ggtcagtttg ttatacaatg ggcaaaggtg tttggtgcat
     6541 ctaagattat agcagttgat atttttgatg aaaaattaga actttctaaa cttctaggag
     6601 caaactatat acttaactca aaagaagtaa atgtaatcaa agaaataaaa aaaataacaa
     6661 atggtggtgc tgatgttgta atagaaactg ctggtagccg atttacacaa gaacaatctc
     6721 tatttgtagc aaaaaagaga ggaaatatag tgtttgtagg tatatcacac acagaattac
     6781 cacttagtgc agacgctact gagtgtatat tacgtggaga acttaccttg aaaggttctt
     6841 ggaattcata tacaagccct tatcctggaa gagcatggac tgcaacattg gattttatgg
     6901 aaaaaggtga cattattttt aaacccatga tttcagataa aataggtctt aatgaagtgg
     6961 gtgacttttt aagtaaaatg agcaagagag aaattaattt caataaaatc ctagtagaaa
     7021 tgtaaaaatt taataaaaaa ttctaaaagc aattaaattt tataactatg acattaaata
     7081 aaataaaaat aattttctta aataaaatat aggaggaaga aaagtgttag aaaatttaaa
     7141 agaagaagta ttaaaagcaa acttagaatt acctaagaaa aagctaatta cttatacttg
     7201 gggaaatgta agtgggatag atagagaaaa aggattagtg gtgataaagc caagtggtgt
     7261 agagtatagt aatatgactg tagaagatat ggttgtagtt gatttagagg gaaatatagt
     7321 tgatggcaaa ttaagacctt cttcagatac cccgactcat ctagttttat ataaagagtt
     7381 tgaagaattg gggggaatag tacatacaca ttcaagttgg gcaacaattt gggctcaaat
     7441 aggaaaatct ataccatctt gtggtactac tcatgcagat tatttttatg gaagtatacc
     7501 atgtaccaga aaaatgacta gagaagaaat ttgcaatgaa tacgaaaagg aaactggaaa
     7561 tgtaatcatt gaagaattta tgggaagaaa tccaattcat tgtccaggaa taattgtaaa
     7621 tgaccacgga ccttttacat ggggaaaaga tgcaaatgag gctgtacaca atgcagtagt
     7681 tttagaagaa gtcgcaaaga tggcttatta tacagagtta atgagtcctg ataatattat
     7741 ggataaggtt cttatgaata aacatttttc aagaaaacat ggaaaaaatg catactatgg
     7801 tcaaaaatga ttatctggag gtgaattatg ctagactctt taaaaggtaa agttgtggaa
     7861 atagcaatta aagcagagca ttatggttta tgtaaagaaa agactggtaa ttttagtatt
     7921 agagataagg atactggata tatacttata accccatcag gtgtaggaag agaagaatta
     7981 aaagttgaac atatttgtgt attggatttg gatggaaatg taatagaagt tgagaaaggt
     8041 attaagccaa gtagtgaggt attgatgcat attgaaatat acaaaacaag agaaaatata
     8101 aatgccatat cacatactca ttctttatat gcaacagcat tttcagtagc aaagaaacca
     8161 attataccaa ttgtatatga atcagttaat tatggcggtt atgtagatat tgcttcatac
     8221 gaaagaccag gaactttaaa attagctaag agtgtttcaa aattactgat agatacagat
     8281 gcatgcttat tagaaagaca tgggttgaca actgtaggta atgatttaga cgaagcatta
     8341 ttaaaatcaa gatatgtgga agaggtagca gaaatatact ataggtcttt agtattaaat
     8401 caatttaaag aacttgaagt agtagatgtt gatgagttta aatcttggaa atacccagaa
     8461 gaaataaagt tttaatctaa ataattttca ttgaagtggt taattaaaat atatgtatta
     8521 tttatatcaa ttactatgtt ttattatatt aattaccgta ttttgtaaat atatttatta
     8581 cattttttca aaaaaatata gaatccaaaa gccatataaa attgacttgg gattctatac
     8641 tttttattta tgcatttttc tttatctgat tttctggaga atttatatgt tcaaaatatt
     8701 ttttagtttc agatactatt ataggtgata atgcaagtaa agctataagg ttaggtattg
     8761 ccataagccc atttacaata tcagcaatta tccaaacaac ttctaacttt aagaaagcac
     8821 ctaataacac catagccaca aatacagttc tatacccatt catgcctttt acaccaaaca
     8881 gaaattcaaa acatctttct ccataataac tccatcccaa aattgtagta aatgcaaata
     8941 atgttaaact aattgtaagt aatattggtc caaatgtagg aattgctccg ttaaaagctg
     9001 cttgtgtcat aacagctcca tttaggtctg aacaccacac tcctgaaata acaagagaga
     9061 atccagtaag agtacagatt ataatagtat ctataaaagt acctgtcata gatataagac
     9121 cttgttccgc tggccattca gttttagcag cagctgcagc tattggagca ctacctagac
     9181 cagactcatt tgaaaataca ccacgagcga taccatttct aattgccagt gacatagttg
     9241 ctcctaaaaa accaccagta gcagcagtag gtgtaaatgc acttttaatt attaacataa
     9301 aagtttcagg gattttattc caaaaagata ttaaaatgat tccacagatt acaacatata
     9361 taactgccat aaaaggtact attttagtag ctacattaga tatactttta agtcctccaa
     9421 atacaactaa agctacaaat actgctaata caacccctgt aattctaggg tctatcccga
     9481 aactattatt tattgcatct gttattgaat ttacttgagt aaaagtaccg attcctaaaa
     9541 gtgcaactag tataccactt atagcgaaaa atattgcaag aggtctccat ttttctccca
     9601 taccatttac tatgtaatac attggcccac cagacacttg accattttta tctttagttc
     9661 tgtattttat tgcaagaaca ccttctgaat acttagtagc cattccaaag aatgctgcta
     9721 tccacatcca aaaaagtgca ccaggtccac cagctttgat agctgtagca acaccaacaa
     9781 tattaccagt accaactgta gcagctagag cagtacataa ggctgcaaaa cttgaaacat
     9841 caccactacc tttgttttca gcagagaata ttaacttaag agctcttggc agtttagtta
     9901 tttgaacaac tccaagctta acagtaagta gtataccagt accaactaat agtaccagta
     9961 ggggtggtcc ccaaataaaa gcatcaactt tgtttaaaat atcaattaga ttcataaaaa
    10021 cactccctca ttttttttgc tttacataat caatagaatt tttaatgaag taattgacag
    10081 gaaataagta taatttatta ataatttcta gtgtaataaa aaaagctaaa tctaaaaaag
    10141 attcagctct ggatgtacac taattagcaa ccaaaataaa agtattagta aatactgttt
    10201 ttggtagata atccctgtcc ttttgcctga gagattgagc ataaaagcct tgccccttcg
    10261 gcgcccataa tgggtctctc cagagttccg taaattttac agtcctactt ttaaaagtac
    10321 ctgagagtgt tactccttcg gtgtgctata tagcaactct tctataaaat cttaaccgga
    10381 tattgatttg tcatagctta ttgtattaat aatattaata taagttttta tgttttgtca
    10441 actaaaaaat taaaaaacaa agaaaaaata taaaaatgta agcctattta cgaattataa
    10501 acttagaaag atgagtaata tacgtgaaaa tgaattttgt aatacacata caaatgaagt
    10561 gtaaaataag ttttaaataa aaaaataata aaatcttaaa taattaatta gatatgatta
    10621 aattatggta atattatagt tacgatatta agaaaagttc tatttagtta gattttaagc
    10681 gtaatatgag attatgtata gagaaaggat gtatttttag atgggaaaaa tattagtgct
    10741 agcagaaaaa ccttctgttg gaagagatat agcaaaagtt ttagggtcaa aaaatgaaaa
    10801 aaatggttat atagaggggc caaagtatgt gataacttgg gcactaggtc atttggttac
    10861 acttgctgac cccgaaagtt atggagaaag atataaatca tggagcttag aagacctgcc
    10921 aatacttccc aagcacctta agactgttgt aataaaaaag agtggaaagc agtttaatac
    10981 agttaaatcg caaatgaata gaaatgatat agatgaaata gttatagcaa cagatgcagg
    11041 tcgtgaagga gaacttgtag caaggtggat aatagaaaaa tcacaggtaa aaaaacctat
    11101 aaaacgtctt tggatttctt caagtacaga caaagcaata aaagacggat ttgcaaaatt
    11161 aaaaagtgga aaagaatatg aaaatttgta ttactcagca atagcacgag cagaagctga
    11221 ttggattatt ggaataaatg caaccagagc tttgacaaca aagtacaatg cacagctttc
    11281 ttgtggaaga gtacaaactc ctactcttgc aatgttgctt aaaagagaag aagaaataag
    11341 aaatttcaaa cctaaagagt attatggatt agaattaatt gctacaaagg gaaattcaga
    11401 tataaaattt atttggaatg ataaaaacaa taattcttca acttttagta aagaaaagat
    11461 agaatccaca ttaaaaaaag taaaaggtgt agacataaaa atatctgaag taaacaagtc
    11521 atataagaaa aaatattcac ctgcactcta tgatttaaca gaattacaaa gagatgcaaa
    11581 taaaatgttt ggattttcag caaaagaaac tttatctata atgcaaaaac tgtatgaaca
    11641 tcataaagta cttacttatc ctagaactga ttctagatat ttaacatcag atattgttgg
    11701 aacattaaaa gatagaataa aagctgtaaa tattagtgat tattcaaaga tttgtaatag
    11761 attattaaaa agcaatatta tagctaataa atcgtttgtt gacaattcaa aggtaacaga
    11821 ccatcatgca ataataccaa cagaacagaa ggtattcatg agtgatttta gtgataaaga
    11881 gcgaaaaata tataatcttg tagtaaaaag atttttagca gtactatctc ctccttttga
    11941 atatgagcag acaactataa gtgcaactgt agaaggagaa atatttaagg ctaagggaaa
    12001 caaagtaata aatttaggtt ggaaagaaag ttataaagat attgaagatg aagatgagta
    12061 tgaaagtata ccaaatgttt tagaaaaaga tgtacttaag gtcaagggat taaagataac
    12121 ttcaggaaaa acaaatccac caccatactt aaatgaggga acattattga ctgctatgga
    12181 aaagaatggt ctagggacag ttgctacaag agcagatata atagaaaaat tattcaattc
    12241 atttttaatg gaaaagaaaa ataaagatat atatataaca tcaaaaggaa gacaattatt
    12301 agatttagtt ccagtagact taaagacacc agaacttaca gcttcatggg aaaagaaatt
    12361 attggatatt tcaaagggaa aacttagtaa aaatacattc ataaatgaca tgaaagatta
    12421 ttcaaaagat attgtactag aagtaaaaaa tagtgataat aaatttaaac atgataactt
    12481 aactaaaaat agatgtccag aatgtggaaa atttatgtta gaggtaaatg gtaaaaaagg
    12541 taagatgttg atttgtgaag atagagaatg taatactaga aaaactatat cacaagtaac
    12601 caattcaaga tgtcctaact gtcataaaaa attagaactt cgtggagaag gtgaggggag
    12661 aatcttcact tgtagttgtg gatacagaga aaagttgtct tctttcaaca agagaaagca
    12721 agaagaaaaa cagaaggcat caaaaaaaga tgttaatcaa tatttaaaaa atcaaaataa
    12781 aaatcaagag acatttaata atccatttgc agaagcatta gctaagctaa aaaacaaata
    12841 attttaaagt tattattata cacaattata actttaactg tatattagtt atatgtactg
    12901 atataatata gtgtatagtt taggcatgaa agtagggctg ttaaatggat ttaagtggca
    12961 tatttaaata ctattgtaaa gagtgtgaaa atacatggaa taattcgagt gttgaattat
    13021 ttgagaatat agaaacgtat agtaaagatt cacaaaaaaa gagggaaaaa gaattagata
    13081 aattgctaaa tacaatatca gttcatttag agaggtatcc aagtgatgct gtattgagaa
    13141 aaatgtgggt aaaaaagggc gaggttttct tacaaaagac attggaaaaa gaaaatattt
    13201 ttaagttaga aaaaatggat gtagaggata gaaaaaaatt tttagatata acaaaacagt
    13261 ttattagaga tgctagaaaa tttgatgatg atttacctat aggtgatatt atgcaagcta
    13321 tgaggaatgt atggatttca aatgcattac aattattatt tggtaaagaa gtatattatt
    13381 caaaagctaa ctttgcatat agtatgttat atccatatac tgataattat ttagacaata
    13441 caaatataga taaaaatgat aagattttat ttaataactg gttagaaaaa aggctcctgg
    13501 gagaacacat taaatctaag gattatcatg aaagtaaagt atctcaaatg atagattata
    13561 ttgaaagtgt ataccctaga gaaaagttta cagaagttta tgaatcgtta ttattaatat
    13621 ttaaaagtca agtaaatagt ttaaaacaac atggtaagga aaatcatttg tgtaaagaag
    13681 atttattatc catttctatt gaaaaaggag gttcatccgt tttagtagat ggatatttaa
    13741 taagtggatt gatgacaaag gaagaaatag agttttgtat aggatatgga tttttattac
    13801 aaatatctga tgatttacaa gatataaaag aagatttaaa atacaaccat aaaactatta
    13861 ttacagagat gtcaaaagag ggtactttag ataaagttgt aaataaacta ataaatttta
    13921 ctattgagtt aatagatagt tttaaaatta ataataaaaa taaatctgta ataactatga
    13981 taaagaatga ttgcttaatg ttaattttat tttctgtagt ttataatgct gaattttttt
    14041 ctgtaggata tataaaagaa gtagagaaat ttattccata tacaatagat tattcattag
    14101 agattgaaga aaaaataaaa gaaaaattta aaaatataga tgttttaaat aatgaaaatg
    14161 aatataaaga aatgattgat attatttgtg cagagtagaa aaaaagggag tgtcttagaa
    14221 tagacttttt agtttatgag gcactctttt tgtataaaat caaatgtatt ttcatcattt
    14281 taataattaa aaagagctta tccctatttg agaaagccct ttttaattta aataaaaatg
    14341 tcaaggttaa actatttaaa tgcagtaata atcccattat catgtaagtt ttttatgatt
    14401 gtagctgcga tagggaatcc aaataatcca gctattccaa atagttgagc tccaacaaac
    14461 atacacatta atgttattaa aggatatagg ccaatttggt gtcctactac ctttggttct
    14521 aatatttgtc taacaacggt tataattatg tatagtatta aaagtcctat tgctaaatta
    14581 atatttccat taatgagtga gataatcatc caaggtgtca aaatacttcc tgtacccaag
    14641 actggcaaaa tatcaacaag tgctatgatg acagatatag taaatgcatt ttcaaccttt
    14701 aggatagtaa gtccaataag tagttctatg aatgtcactg atagtagtat tgcataggct
    14761 tttagtagtt ttaaaagagc attaatactg ctatttttta tatttattat cataaattga
    14821 cttttttctg gaaattgttt taagataaag tttactattt tttgatagtc aaaagtaaag
    14881 aaaaaagaag caattaaagt aataataagt tttaacataa atgcaggtgt tttacttgca
    14941 atactagcaa tagcattgag tgcatttgtt gaaatagaag ctactaaaga catcatagat
    15001 tgacttatat tatcccaacc aagtattaat gctaggttaa cattaggtgt actagcatct
    15061 atctttgaaa ataaagtatt tagcgttggt tgtatacttg atttatatat ttcaggaagt
    15121 ctaaaaaata catcacttac acttgcaaat atcttcacac caaagctaac taataaaaat
    15181 atacacaacc cataaaaaat taaaagtatt actatagaaa taagtgtacg atttaaattt
    15241 gtattatttg taataagtct tataataggt cttaagatgg atgcaatgat aaaactgatt
    15301 acaaaaggca ttaataatgg caataagaat tttaatacaa tatatacaat acttgttatt
    15361 aaaactacat atgtaaattt aattataaaa ttcttttgct tttgaatatc cataatataa
    15421 aacctccaat gtatttgata ttattctaaa tatggtcttt tttacagaaa atatactact
    15481 gtatattgat tataaactat aaagcttatg taaagtaaat aaattatgta aaatttaggt
    15541 taa
//
LOCUS       DAKBNN010000087        15457 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000087 DAKBNN010000000
VERSION     DAKBNN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.087"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(293..862)
                     /locus_tag="OUB02_002216"
     CDS             complement(293..862)
                     /locus_tag="OUB02_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT8885973.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            1065..1292
                     /locus_tag="OUB02_002217"
     CDS             1065..1292
                     /locus_tag="OUB02_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT8885974.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1511..2254)
                     /locus_tag="OUB02_002218"
     CDS             complement(1511..2254)
                     /locus_tag="OUB02_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCT8885975.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAAPQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2422..3294
                     /locus_tag="OUB02_002219"
     CDS             2422..3294
                     /locus_tag="OUB02_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8885976.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3294..3959
                     /locus_tag="OUB02_002220"
     CDS             3294..3959
                     /locus_tag="OUB02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCT8885977.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3980..4837
                     /locus_tag="OUB02_002221"
     CDS             3980..4837
                     /locus_tag="OUB02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8885978.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEKGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4993..7539)
                     /locus_tag="OUB02_002222"
     CDS             complement(4993..7539)
                     /locus_tag="OUB02_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8885979.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7539..8225)
                     /locus_tag="OUB02_002223"
     CDS             complement(7539..8225)
                     /locus_tag="OUB02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885980.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8308..9297)
                     /locus_tag="OUB02_002224"
     CDS             complement(8308..9297)
                     /locus_tag="OUB02_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8885981.1"
                     /translation="MFRNREFRKLAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9284..9970)
                     /locus_tag="OUB02_002225"
     CDS             complement(9284..9970)
                     /locus_tag="OUB02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8885982.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10470..13676)
                     /gene="carB"
                     /locus_tag="OUB02_002226"
     CDS             complement(10470..13676)
                     /gene="carB"
                     /locus_tag="OUB02_002226"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCT8885983.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(13869..14918)
                     /gene="carA"
                     /locus_tag="OUB02_002227"
     CDS             complement(13869..14918)
                     /gene="carA"
                     /locus_tag="OUB02_002227"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCT8885984.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(15176..>15457)
                     /locus_tag="OUB02_002228"
     CDS             complement(15176..>15457)
                     /locus_tag="OUB02_002228"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885985.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
BASE COUNT         5146 a         2703 c         2032 g         5576 t
ORIGIN      
        1 gatataccta tttttatatc acaatataaa taattacata taagcattag tttttttatc
       61 ataaatagat ttttcaagat ttatcatttt ttcataaata tattatagtt aaatatttta
      121 atattaccta acatctacaa taaaatatac tcttaaaata aaattatata attaaaaata
      181 ctctataaaa gctaaaaagt ttttctattc gctacatcta aaaataatta gatagaggta
      241 taaatgtatt tagtttattt atacctctag tcttttataa attatatctc tactaaattg
      301 attttttagc acgaataagt gtgctcttgg agataccagt catcttcaca acctctctat
      361 atggcttaga atttaataac cccaatgcat attgtatctg ttgattgctg tatttctttg
      421 gcctaccatc tttaaagtcc tttttctttt tagcaaattc ttttcctaat tgagtacgtt
      481 ctattatcat atttctctca agttctgcaa ctgcaagtaa tgtagtcaaa aagaatttac
      541 ccattgaagt attttctaaa aggcctatat ttaaaatatg aacacttact tctttagcaa
      601 ataaaccttc tattagttgt attccttctg atgtacttct agctagtcta tccagcttag
      661 ttgcaacaag taagtctccc ttttttagag atgatataat tttttcaaat ttaggcctgt
      721 ttgtagtagt gcctgtataa gtttccttta ttattttagc atcattatat ttattcaaaa
      781 tttcttgctg ttgctgatac aaactgttgt tgtctgtttg attttttgta cttacacggc
      841 aatatccata tataataatc atcacttcct aacataaaga ttttgtacag aagttaccca
      901 gaagcttgtt tttacttatc tttatccatt ttactaaaac gatttttggg tgtcaatact
      961 gttaactttt gacaccataa taattccttt ataatttgtt tgactatgtc aaaaattacc
     1021 aatataatga aattaatgag tttatacaaa ttggaggaaa taatatgaat agtctgttaa
     1081 aagaagcaat agatgaatta aaaaacatcc attgcgggga agttttttta ttaaaagaat
     1141 tgttcaaagg ctatgaatgg aacaggttgg agatgaatat cagattgaaa ttaggaaaac
     1201 tttttttgta tgaagtggta actaatccca atttaaatgt agaggtcatc aataaaaatt
     1261 ctgcttatca gcaaatgtat aaaaaaaaat agtgacttaa atgtatataa tagtataatc
     1321 ttcttattga ttcaaaagtt aaaactttta atacaaaaca atcattctat tttaacattt
     1381 atcaatatgt ctaaatttat ataattaaca catacgaata gtattgttta aatcacatta
     1441 tttaagatag ataaatctta accctactcc agcataaata ctaaaatagg gcttagaatt
     1501 atctatattt ttatcccata aaatgcttag ctcttcctct tgattgaaca tatatatctg
     1561 gggaaataca atagaagcgt aaatttataa aatttccaaa gtcagaaaac ttcagttcaa
     1621 acttaggtat accaaaactt tcaggtgcta tttcgtcaac accaaaatag attataattc
     1681 cattatctgt aagataaaat gcttggtctt caggtataac taactcaaca ttagaataat
     1741 acatttctct attttcattt atttgagatt taatgtaatc agtaattata ttaatataat
     1801 caactccatc tctaaaaatg tcattaagta aaagttcttt tccagttgtc aaatctatat
     1861 tgtaattatt caatgtattg tatttaggcc catagttatt agcaaacccc ataaggttta
     1921 atatcatact cagtacataa tttttattaa aagtaactgc gtaactggta gatacccaat
     1981 agctttgttt tggaagtgaa ttttgaggag ctgcgttatt atattctcgt tcttcttcct
     2041 gtaagccatt tttaaaatta tttacagtca ttcttacagc attgtttatg ttatttaaaa
     2101 taactgggtt tacatacgtg gattgatttc taatattgta tataacagga tactttacat
     2161 catatgaaaa atacttactt ctttcaaaat cttcattaaa ttgtacatat aatatattat
     2221 atgtacaggt atttgtagat tgataatttt ccattaaaat cctccccctt ttataaaaaa
     2281 atcagataca atataatatg cttaaaacag taataatgtt aatggtaaat gtaaagaata
     2341 gaaagtatct aaaacagcaa aaaatgacta attttgtggt ataatacata ggattgagag
     2401 ttattttata ggaggaaaaa tatgttatta ttagcagata aatggaaaga ttatgaactt
     2461 atagatatgg gaaatggaga aaaattagaa cgttggggaa gttatgtact tagaagacca
     2521 gacccacagg ttgtatggcc aatggaatct gaatgggctt tatggaaaaa ccctcatgga
     2581 cattatcaca gaagcaataa aggtggaggt caatgggaac acaaaaaaag atatccagaa
     2641 caatggacta taaattataa aaacttaaag tttcacatta aaccaactgg ctttaaacat
     2701 actggattat tcccagagca agcagctaac tgggattgga tgatagagaa aataaagaaa
     2761 gctaacagac ctataaaagt gttgaatctt tttgcatata caggtggagc tactgtagca
     2821 tgtgcatctg ctggtgcaga agtatgtcat gttgatgctg caaaaggtat ggttaattgg
     2881 gcaaaagaaa atatagaatt atcaggtctt aaaaatcaaa cagtaagatt tatagtggat
     2941 gatgttgtaa agtttgttga aagagaaata agaagaggta aaaaatatga tgcaataata
     3001 atggaccctc cttcttatgg aagaggtcct aaaggagaag tatggcaaat agaagaaaag
     3061 ttatacagtt ttgtagattt atgtatggga gttttatcag ataacccttt atttttctta
     3121 attaattctt atacaacagg attttctcca atagttcttg aaaatgtatt gtctacaaca
     3181 ataggtaaaa agacaaatac aggagatata tacggtgggg aattaggaat accagcttcc
     3241 agagatggta agatacttcc ttgtggtata tttggaagat gggagaaaaa ataatgataa
     3301 aagtcatata tgaagataac catttattag ttgtagaaaa accagttaac atattgtctc
     3361 aaggagataa tactaatgat aaagatatgg taaatctttt aaaagactat attaaaaaaa
     3421 agtataacaa acctggaaat gtatatattg gtttaataca tagattggat agaccagtag
     3481 gtggagttat ggtgtttgca aaaacatcaa aagcagcatc tagattgtct gagcaggtaa
     3541 gaagtaagac tttcaaaaaa acttatctag ctgttgttca tggcaagata aaatctaact
     3601 cagatatact aaaagatttt ttatataaaa ataaaaaaac taatatggta actgtagtga
     3661 aaaaaagcca taaagatgcc aaagaagctg aacttaacta taatgtatta gattttaaag
     3721 aaaatctaag tttagttcaa gttaatttaa agactggtcg ttctcatcaa ataagagttc
     3781 aatttgcaag cagaaaacat ccattatacg gagaccaaag atatggcaaa gaaataaata
     3841 agattggaca acaaatatct ctttggtcca ataagataga aatttgccat cctactacaa
     3901 aagaaaaaat ggaatttaca tgtcatccac ctgacaaata tccatggaat atattttaaa
     3961 tctcaaatga ggtgattata tgagtaaaaa attttcaata ggagaaatgt ctaaacttca
     4021 taaaataagc atacagacac taagatacta tgaccaaata gatttattta aacctgttca
     4081 tgttgaccaa gaaagcaatt acagatatta ctcaattgac caattttcac aattagatat
     4141 tataaaatat ttaaaatatt taggtatgcc tttaaaagaa attaagtcaa agcttgatga
     4201 aaataatgaa gaaattctaa aattactaac aaataaaatt aatatagtgg atgaaaaaat
     4261 taaagaatta gagcttataa agaaggtact aagcacaaag aaagaaacta tggaaaaagg
     4321 tatgaaagta gatgaagttg gtaaaatagt gagaaaacat attccacgaa gaagtatctt
     4381 atctatagag tatgaaaaag gattaagtag tgaagaaact atggaacttt ccagaagaca
     4441 aatagcaaat atattagaag ataatatatc tgtgttctat ggtggagtaa gtggacttgt
     4501 atctatagat aaatttttaa aagaagataa agttatttat gacaatagtt ttgttactgt
     4561 tgaaagagac ttatttgata taaaatcaca aaaacatata acagaaattt catctggtga
     4621 ttttatttgt atgacatata aagggcctta caaagaaaat tataccgctt ttaagaaact
     4681 ggttgattat gttgaatata aaagaatacc tattgaaaat tatatatatg aaatacctat
     4741 aatagaccct ctgtctacaa acaacgaaga ggaacttcta acagaaattc aaatacaagt
     4801 taaaaagaga gaaattgact taagatgtct agtgtagtaa tctagtttac ttacacatct
     4861 taatatagtt gtctattatc aacatatgat ttttctagat agtgattcct aacagtgaga
     4921 ttttcaaaaa ataggaggaa atatggctaa tgctaattcc ctcctatttt taattaatcc
     4981 atatcatttt aattaagcca tgtcatcacg aagtgcatca ataatatttt ctttttttat
     5041 cttattaata gtatacaaca ttgtaataaa tactataaat aaaacactaa atatactaat
     5101 tccaatacta aaccatggaa tcacaaaatc aatatcatct gtaaccatta ctttatgaat
     5161 tagccatgaa gaaattactg ctataggaag tccaaagaac aatgctctca tgccataaaa
     5221 ggcacactca aaattcatca tcttttggaa atcatgctca gacattccta cagagcgaag
     5281 catggcaagt tctctacgac gtaacttaat attagttgaa attgtattaa acacattggc
     5341 aatcgaaatc agagaaatca taattatgaa agtataggca aaaacattgg caataaaaat
     5401 gtaattacgg ttctcatcca tcattccata catattgtac agattgtact cattcgtaac
     5461 tccctcactc tcaataattt gctccatttc tgcaactgac tgagtaggat ttttcgattg
     5521 aaagcttaaa ctttcaattc ctgtagaaac accaggaatc tcaaacttat ctttggctga
     5581 ataaggagcc aatactctga agcttgcttt cttcacatct gaacttctga ttcctggagg
     5641 agaatcaggt ggcactactt tgacaaatga aatattcaca ttttgtctct caggtatctc
     5701 caacttgcca ttagtttcag gagtgaggct aacattcata gaagaactgt tgaaaatatc
     5761 aataatatta ttatcctttg ttaccataga aacagcaatc agttttgcat tttgtccagt
     5821 atattcttct gctggtaatc caagactttt aataatattc agataagttc tatcatcaag
     5881 aaattgcata tctactggta attcaagcat ttcactcata gaatgtgaac cctcatagtt
     5941 caaataatcg tctgtaagct cacttacttt ggcaacacat gaataggtca taagtttctg
     6001 atacaaacct tcgtaaacac catttgcagt ttttagctta tcgtaaagct tcaacatttc
     6061 gttgtctttc ataccttgtg tagaaaaaat aatatcctgg tcagtaacct ctgacctctg
     6121 atttgacatc tgttttaggt ctgctactaa agcacttgta gatataaata gaactatgct
     6181 taaaacaagc gacaatacaa tacttcgata acgtttcttg tttcttttaa agtttttcat
     6241 agcaagaact ccctcaagac cataaactcg ctgtgttagt tttgatgtct tcacagattt
     6301 agattcaatt tttacctcat ttgtctgacg aatacactcc atcactggtg tgttggcagc
     6361 ctttcttgct ggtatgtaag cagaaattaa gattgtaacc atacttacca ctgctgcaac
     6421 gatgattgca gggatagaca atgttaatgt caacggaacg tttacataga gaatatttgc
     6481 aaagttttta gctatgacag aaatcacaag tccaatactg actataccga ttgcaacacc
     6541 aattggtata ccaatcgcac caatgcaaat tccttcaaat agcaccgaat ttcgtaactg
     6601 ttttgctgta gctccaacag atgaaagtat tccaaattga tgtgttcgct cgttcaatga
     6661 catgttaaat gaattatata ttagaaaaat tgagcctagc atgattatgg caagcacaat
     6721 gccaccaacc gagtacaaaa acatattgaa caaattgtta tcagaaacac ctatgaagcg
     6781 caacacattg tcattgaaaa tggaagcatg gtctttagtg ctacttatgt aagagcgaat
     6841 ctgatgtgga tttttcaatg tgacaaaaag gctaagactg tcagtcttga cttgtttatc
     6901 ttctttggtt atcaaggtat atcctggtgc agaagattct tcaaatacgg gctttctaca
     6961 gatacccaca actgtataag ttttcttatc ctgtggcaca aaaacttcac tcccagcagt
     7021 gtatgggtca ctctgaccaa gcttttcatt ttcactcatt cgtttcccta catccaaaga
     7081 aatagtatca cctactgcaa atttgacacc accatctgtc gcaactttac ttgaaacaag
     7141 aatctctcta ctattttcag gtaatcttcc agaaattaaa gttataggca aagtatcaaa
     7201 agcttctttg ctaaatccag caataaaaag atatggttta tctggatttc tgttaccttc
     7261 aagtgttgca tagccaatat tttcaaatgt tgcagtattt gcaacttcat cattgactct
     7321 ttgttcctgt acaaaagaag aatccacatc caaaaactgg acatgccaat caccatattt
     7381 tataatagct ccatttgtca tatagttcat caaagaaact ccaaaggtag taactgctgt
     7441 aatcatagca gctgacagaa caactcctat aattgttaca agagttcgag tgcgattctt
     7501 tttcaatcct tgcagagcaa ctttgttaaa aatgttcatt aacgattcac cttcctctca
     7561 tcacgcacta cttttccatc tgaaatacag ataattctat ctgcttgtaa agcaatgttt
     7621 tcatcatgtg tgacaataat gagagtttgg tgatatttac tatggctctc tttcagtaac
     7681 tggataatct cttgtccatt tttactgtct aagctacctg taggctcatc agcaagcata
     7741 actgctggtg cattcatcaa tgcacgacca atggcaacac gttgctgttg gccaccagaa
     7801 agttgatttg gtaaatgtgt ttttcgttct tttaaaccaa gtaattctaa caagtcattt
     7861 aaccgttctt gattgacctt acgtttatcc ataagtatag gcaatgtgat attttccact
     7921 acatttaatg tgggtatcag attgtgaaac tgataaatca atccaacttg tcgcctacga
     7981 aaaatagcaa gtttttcatt gctttggcca tatacatctt gcccctccag atatatcttt
     8041 ccacttgttg gtacgtctac tcctgcaatg gaatgtagta gtgtagattt accagaacca
     8101 gaagaaccaa tgattgcagt aaactcccct ttttcaatag taagtgagac atggtcaagt
     8161 gcagtaacct tattttcatt cttaccataa accttacata aatcttcaat ctttaaaaac
     8221 gccattatat aagtctcctt tcatatttta taaatatatc ataggacttt caacttacat
     8281 ttaagtgaca tttaaatgag agattcgtca ctttggaaaa cgaataacaa atattgcacc
     8341 accttgtggg tgattttgtg cagtaattgt tcctccttgt ctcataataa tcgtcttaca
     8401 aagtgccaat cctattccat aaccagctgt gcttgaattt ttcccacgat aaaatctgtt
     8461 aaatagacat ggtaagtctt cttttttaaa gcctgctccg ttgtcatgaa tagtaagttg
     8521 agtaaacaaa aagttgtcct cacaaatgat atcaattttt ccattatcac ccacactctc
     8581 catgcagttt ttaaatatat tttgaattgc ttctgaaagc caatttaaat cacccaaaat
     8641 ccttactcct tttggtatat caatatgtaa agtgatattg tgcagttcca taggaatcaa
     8701 aagttgatga agtgcagaac taatcaaatt attcacatca atttgttctt tttgaaagac
     8761 tacaatacct gcatctaaac gtgataattt taaaagggaa gtaatcagcc agtccattcg
     8821 tataagtaat tcctctgttt ctcgtatcaa ctcttttctc tcatcttcat cagaagtatt
     8881 ctctaacagt gacaaaataa gagttactga tgtaagagga gtgcgaagct ggtgggctat
     8941 gtcagctagt gagtcagcaa ggtgttcttt ttcttttttc agtgcgtaat tttgctctct
     9001 aatacgtaac gtcattttag ttatttcact ttgtagaata gaaagctcac cctcatctga
     9061 ttcagcaata tacagatggt tagcattatg aagcacaaga ttaatttgtt ctgaaatctg
     9121 tgcaatactt ttgtatcgat ttttagtaaa cacaaaaaat gctattccaa aggtagtggc
     9181 agaaacaata gaaagtattc cagtcataat actgattgca aatcccaggg ctacagtgag
     9241 aatagtaatt aataagaata aaatagcaag ttttcgaaac tctctatttc gaaacataac
     9301 ttccccccaa tctataccct gtcccacgaa cagtcaaaat tatttgtgga cttgaagggt
     9361 catcttctat cttctctcgc aagcgtttga tgtacacagt caatgtgttg tcattaacaa
     9421 actcacctgc cgcatcccac aattcatcaa gtaatcttac cctcgtaata atacttttag
     9481 gattgctaat aaacaccaat aacaaacggt attctaaagc tgaaagaaaa acctcattgc
     9541 cattcttttt cacaacaccg gttgctgtat ctacataaag cccacaaatt tcaaaagttg
     9601 acccaaaacg cccacttttt cgtagtgctg ttctaattcg cgcaatcagt tcacgtggac
     9661 gaaaaggttt agtaatataa tcatctgcac ccatattcag tccagtaaca acacttgatt
     9721 catcaccgga agctgtcaaa aagataacag gaatgtcttg cttttctttg atttctgtgc
     9781 aaatcgtaaa cccatttcca tcaggtaaag aaatatcaat aagtgccaaa tcaaatttat
     9841 tcttagcaat ctctacaagt gcttcacttc ttgtggatac atgggttact ataaatcctt
     9901 ctgaattaag caaaagaata agatttttgg caattgcttt atcatcctca actaaaaata
     9961 tccgtttcat ttactttcat cctcctttat tttattagtt ttatatattc cagcagagca
    10021 atagtacact actattttac ttatcattac acaattttca gtagtttctt ttgttcttat
    10081 ataatccatg cataatacct ccattgattt attatatttc ttataacata aatacacaag
    10141 ataagacaaa aaacataatg taagtgcaag ttttttaact cactaactta taaacataca
    10201 ctcctactaa cactactttc ttgatgacac atcttacaag cttatattca tacatcaatg
    10261 aaaaattaaa tttatccatt tattaaaata aaaggcaata gcttctatag ctaatgccct
    10321 ttatcttaaa atgatttaaa tactatacaa tttttataaa tggctataaa taaactcata
    10381 tagaatataa aatttatcta cagtcttttg ttatacaaaa tttataacct cttatagaac
    10441 aaatttacca ctttgcaaac ttatcacttt tactcagcta tattataaac ttttaattta
    10501 tctttgttta aatgtttctt cttaacttct actaaagctt ttagcgtatc cagagatgtc
    10561 attatttcag tagaaaattc aatagctgtt cttctcattt taaagccatc tcttgtagag
    10621 tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct tatagcatct
    10681 aatatgtttg gtctaggttc ttcaattcta tttactatat cagcctctat tccattttct
    10741 cttaaactct tcgctgttcc ttctgtagca acaaaagtat atcctaattc tttcatatct
    10801 tttgctatct ctataaactc atctttatca tgattattta cagtagcaag tactactcct
    10861 ctttcatcag acatatgtct acctgctgcc aagaaccctt tatataaggc ttcttctaaa
    10921 ttttctccta cacctagaac ttctcctgta gacttcattt caggtcctag acttacttca
    10981 actttagata atttagacat tgagaatact ggtactttta ctgatactaa ttttggctct
    11041 ttgtaaactc ctgtaccata tcctaaatcc tttaatttag ctcctaacat acacttagta
    11101 gccaagtcaa ctattggtac tccacttacc ttacttatat atggaactgt tctactagct
    11161 ctagggttaa cctcaattat atataattca ttttgaaatt ctataaattg tatattaacc
    11221 ataccaagca cattaaggtc taaagctatt tttttagtgt attctactat cttttctttt
    11281 atttcatcag atatattttg acttggatac attgttgtac tatctccaga gtgaactcca
    11341 gctctttcta aatgctccat tataccaggt atcaatatat cttctttatc acatatagca
    11401 tctacttcta tttctctacc agttagatat ttatctataa gtactggatt tttatggtct
    11461 ctatcaaatg catcatccaa atactgaatt agtttttctt cgttataagt tatttccata
    11521 ccttgaccac caagtacata agatggtcta actaatactg gataacctaa ctccttagca
    11581 atttcaacac cttcattagt tgtccataca ccttttcctt ttggtctatt tatatctagt
    11641 ctctctagta aatcatcaaa tttttctcta tcttctgctg catctatatc tctaaagtct
    11701 gttcccagta taggtatatt tttttcatgt aaaaacttag ccaattttat agctgtttgt
    11761 ccaccaaact gtagtattac accttctggt ttttcttttt ctattatatt taaaacttct
    11821 tcttctgtta atggttcaaa atacaactta tcagatgtat caaaatccgt acttacagtt
    11881 tcagggttat tgtttacaat tatagtttct attcccattt ttcttaaaga ttttacacaa
    11941 tgaactgaac aatagtcaaa ttctattcct tgaccgattc ttattggacc agaacccaat
    12001 actacaactt ttctcttatc actaactact acctcatcat attgttcata agttgaatag
    12061 taataaggtg atagagcttc aaattctcct ccacatgtat caaccatttt ataaactggt
    12121 tgtatattat ataaacttct caattcatat aatttttctg ggcttatctt cattaagtca
    12181 gatatacctt tatcagaaaa tccctttttc tttaattcat gtaaatattc tttatctaag
    12241 tcttctattt ttagaacttt taatttttct tcttgttcta ctatccattt aaatttattt
    12301 ataaaccact tatcaacacc agttatttct gatatcattt ctactttata gcctcttctt
    12361 atcatttcag ctaagtcaaa tagtctctca tcatcaggta ctactactct agcctttaat
    12421 tcttctaatg ttctaccttc tgatggagca tgaaccaatg aatattttcc tgtttcaagt
    12481 gatcttatac ctttaagaat agcagcctca aagttacttc ctatactcat gatttcacca
    12541 gttgccatca tcttagttcc taattttcta ttggcatgtc taaatttatc aaatggccac
    12601 tttggtattt ttactaccac ataatctaat gttggctcaa aacaagcata agttttttta
    12661 gttactgcat tttctatctc atccaaagta taacctaaag ctatcttagc tgctactttt
    12721 gctattgggt atcctgtagc ttttgatgct agggctgatg acctactaac tcttgggttt
    12781 atttctatta tcgcatattc taaactatgt gggtttaaag ctatttgaac gttacatcca
    12841 ccttgaacct ctattgcatt tattatgtct attgatgcct ttctaagtag ttgatactct
    12901 tcatcactta gagtttgact tggagcaaca actatactgt ctcccgtatg aactccaact
    12961 gggtctacat tttccatatt acatacagtt atacagtttc catttccatc tctcattact
    13021 tcatattcta tttctttcca accttttata ctcttttcaa gaagaacttg tccaacagga
    13081 cttagctgta gtccatgtga aagtatttct cttaattctt cctcagtatc agctatacct
    13141 ccaccagttc cacctagcgt atatgctggt cttacaacta ctggatatcc tatcttattg
    13201 gcaaactcaa gacctgcttc taaatttgta actatatcac taactattac tggttgattt
    13261 atttctttca ttacttctct aaataaatct ctatcttcgc ccttttttat agattctata
    13321 gatgttccta taacttttac attatattta tctaatatac cagcatcatg taattctaca
    13381 gctaagttta atcctgtctg accacccata cctgcaagta aactatctgg tttttctttt
    13441 tctattattt tttctataaa ttctattgtt aatggttcta tatatatttt atctgcaatt
    13501 tctttatcag tcattatagt agctgggtta ctatttacta atacaacttc aattccctct
    13561 tcttttaatg cttggcaggc ctgtgttcct gaatagtcaa actctgcagc ttgacctatt
    13621 attattggcc ctgaccctaa tactaaagtt ttctttatac tatctaattt aggcataact
    13681 ctttctcctt atctaaacaa ttttcacagt tcttatcttc atcatctata caatcaactt
    13741 taattacttt attttatctt aagttcaaat ttactcatca atacatctat ttaatacatc
    13801 tatgttaatt aaaccagact ttttgattta agttattctt gttttttagt tagctattct
    13861 aatgttgttt attttactaa ttctaaaaat ttatcaaaaa tatagtcatt atcttttggt
    13921 cctgggcaag cttcaggatg gtattgaaca ctgtatacat ctagcttctc atgtctcatt
    13981 ccttcaacag tattatcatt taaatttata tgtgtcactt tcatttgttc aggaacttct
    14041 gaaacataat aaccatgatt ttgagaagtt ataaatactt ttccttcttc taagtcttta
    14101 actggatgat ttccacctct atgcccaaat ttaagtttag cagtttttcc tccaagagct
    14161 aaagctaaaa gttgatgtcc taagcatatt ccaactatag gttttttacc aataagagct
    14221 tttatatttt ctataacatc ttctaagtct tctgggtctc caggtccatt tgacaagaat
    14281 attaagtcag ggtttatact caatacatct tctggagatg ttgtagctgg aaatattgtt
    14341 atatcacatc cacgagctat aaatgatctt aatatatttc tttttactcc aaaatccata
    14401 acagccactt ttggaccatt tccttttata tgctctactt cttttcttgt aacagttcta
    14461 actgcttctg tatttgaaaa cttatccaat ttatgtttaa catcttctaa tttactgtct
    14521 tctaatgtaa ttataccttt cattgttcca ttatttctaa gtatcttagt caaagctctt
    14581 gtatctattc cttcaagtcc gataacttta ttttgcttta aatattggtc tatatcaatt
    14641 tcacatctaa aattacttgg agaatcactt ttctccctta ctatgaatcc ctttacttga
    14701 actttttcag actctgctga tgataaattt ataccataat ttcctacaag aggataagtc
    14761 atagtaacta tttgaccata gtaagaaggg tctgttaaaa cttcaccata tcctatcatc
    14821 gaagtattaa atactacttc tcctacactt tcttctaaat atccaaatgc ctttcctata
    14881 aaaactgttc catcttctaa tattaacctt gctttcatac tatagtacct cctataattc
    14941 tctttagaca aactaaagaa cattaactaa atataagctt ttaaatatct atatctattt
    15001 ttcactttta cattctagtt ttatacttat ttacatttat tttttatctt tttctattat
    15061 actacaaatt tcttaaaaaa aagaagttat ctcaaaagta aaaactccta gaaaaaataa
    15121 cctctttttt tttatttaat aattaaattc ttaaactata caatcaactt aatttctatt
    15181 cagctatatt gtaaactttt aactcatctt tatttaaatg tttcttctta acttctacta
    15241 aagcttttag tgtatccaaa gatgtcatta tttcagtaga aaattcaata gctgttcttc
    15301 ttatcttaaa tccatctctt gtagagtcat ttcctttagt tggtgtattt attactatat
    15361 caacttgttt atttcttata gcatctaata tgtttggtct agattcttct actctattta
    15421 ctatatcagc ctctattcca ttttctctta agctatt
//
LOCUS       DAKBNN010000088        15445 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000088 DAKBNN010000000
VERSION     DAKBNN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.088"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            17..724
                     /locus_tag="OUB02_002229"
     CDS             17..724
                     /locus_tag="OUB02_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8885986.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            771..2366
                     /locus_tag="OUB02_002230"
     CDS             771..2366
                     /locus_tag="OUB02_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8885987.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKVLMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            2469..3551
                     /locus_tag="OUB02_002231"
     CDS             2469..3551
                     /locus_tag="OUB02_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCT8885988.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            3700..3861
                     /locus_tag="OUB02_002232"
     CDS             3700..3861
                     /locus_tag="OUB02_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HCT8885989.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            4202..5470
                     /locus_tag="OUB02_002233"
     CDS             4202..5470
                     /locus_tag="OUB02_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCT8885990.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            5665..6453
                     /locus_tag="OUB02_002234"
     CDS             5665..6453
                     /locus_tag="OUB02_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCT8885991.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            6453..6902
                     /locus_tag="OUB02_002235"
     CDS             6453..6902
                     /locus_tag="OUB02_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCT8885992.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            6906..9170
                     /locus_tag="OUB02_002236"
     CDS             6906..9170
                     /locus_tag="OUB02_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCT8885993.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIETSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            9310..9621
                     /locus_tag="OUB02_002237"
     CDS             9310..9621
                     /locus_tag="OUB02_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8885994.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            9907..10407
                     /locus_tag="OUB02_002238"
     CDS             9907..10407
                     /locus_tag="OUB02_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCT8885995.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            10464..11450
                     /locus_tag="OUB02_002239"
     CDS             10464..11450
                     /locus_tag="OUB02_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCT8885996.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            11500..12222
                     /locus_tag="OUB02_002240"
     CDS             11500..12222
                     /locus_tag="OUB02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HCT8885997.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            12251..14185
                     /locus_tag="OUB02_002241"
     CDS             12251..14185
                     /locus_tag="OUB02_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8885998.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYISEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMALNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            14178..>15445
                     /locus_tag="OUB02_002242"
     CDS             14178..>15445
                     /locus_tag="OUB02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HCT8885999.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKHLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         6127 a         1437 c         2680 g         5201 t
ORIGIN      
        1 aatatggagg gaaattatgt taaaagtaat caatgtatct aaatcatata aaaataaaaa
       61 agtagtaagt agtgtctcat ttgatgtaaa tgatggagag ctttttgggt ttataggaca
      121 taatggagca ggtaaaacta ctactattaa agcaattgtt ggaatacacg attttgaaga
      181 gggagaggta ctaataaatt ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga
      241 aatggcatat atacctgata atccagattt atacgagtca ttaactggta tacaatacct
      301 taattttata gctgacatat ttgaagttgc caaagataaa agagaagagt taataaaata
      361 ttattcaaat aagtttgaac tcaataaagc attaggagat ttaatatctt catattcaca
      421 tggtatgaaa caaaaattag ctataatttc ggctttgata cacagcccaa aaatcttaat
      481 tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc tttatattga aggaaattat
      541 gagggagttt tgtgacaaag gtggttgtat atttttctca acacacgttt tagaagttgc
      601 tgaaaaaatt tgtgataaga ttgccataat aaaagatgga aatataattg cttatggaac
      661 tacagaagaa gttaagggaa gtaattcact tgaaagtatt ttcatggaat taatagagaa
      721 ataaaatgtt ttttatacaa tatagtgtct aaagtgagga atgataaaat atgagcaatc
      781 taagtatatt aataaaaaat aatataataa atgaatttaa attaaatgcg ttaaaaaaag
      841 ctgacggtag agaaaaacaa aaaataatgg gaatgatgct ttcagttgtt ataatagcgg
      901 gtctattaat tttttatgta acgagcttaa gtctctcttt agttgatatc ttaaagcaaa
      961 taaatcaact ggaaatgcta ctgatatttg gttttggatt atcaacatta acaactattg
     1021 ttacatcatt atataaaacg tcatcatatc tatttcaagc aaaagattta gaccttttga
     1081 ctagtttacc tataaaagaa tctactgtac tagcaagtaa gatattaatg ttggttggag
     1141 caaactattt attttcttca atatgtatga ttatacctgc agtggtttac tttttaaatt
     1201 ctgatgtaag tataacttat ttcccttatt tgatattgct atttttagct ttaccattat
     1261 taccgatagt tgcttcatca ataatttttc tatttatagg gcagatatca tcaagaataa
     1321 aatacaaaaa tttagtatta ataataggta gcattatatt gatgttgctt tataccctac
     1381 ttatgtcaag acttaattct atgtcagttg aaatgctaaa gaatagtgtg tctatttcag
     1441 agacgattag aaaagtatat cctcctatat attattttgt tgatgctctt aaaacaggaa
     1501 gtatagtttc atttttagca tttatagcca tctcaataat accatttttt atgtttgtaa
     1561 ctctgtttgc aaaaaaattt aaagatataa atgctaagat gagtgaagga tataagtcaa
     1621 aaaattatga atttaagggt caaaaaactt caagaccttc aaaagcttta ttaaaaaaag
     1681 aatttaagag atatacttct tcgtttatat actttttaaa ttcatctgca ggtttgttat
     1741 ttctaatagt aggtacaatt ttaatattat tatttggagc agataaagtg ggtgagttac
     1801 tgaaattgaa tatagatttt aatcttataa aaatacaatt attgggagta ttcttcttta
     1861 taatagtaat gaattgtaca acatactgtt caatttctct tgaaggaaaa aatttatgga
     1921 tactgaaatc ttctccaata gatgaaagag aaatattctg gtcaaaagta ttaatgaatt
     1981 ttttattgaa tgccccatta tctattttat gtctaatatt agtatctata agattagact
     2041 ttgaaattca gtttattatt gtatcaatct gtttgattgt aagtttttca atgtttgtag
     2101 cattgatggg attattatca aatttattat acccaaacct tgattggaaa aatgaggtgg
     2161 ctgtagtaaa aagaagtgca agtatgatag ttacaatgtt agtatcatta gcttatgtaa
     2221 ttatacttgg aggtatatat gtattacttg gaatttcgtt tttaaatata tatattattt
     2281 tggcaagtat acttactttg atattagatt ttatgatatg gagaattatt tctactaaag
     2341 gagtaagaat atttaaaaat ttataggtgt atttatgtca aataaagact tatctttttt
     2401 agtcttgcaa ataataggtt aagtagtata ctatgtatag tgatatgaaa aaatttagga
     2461 gaaaataaat gagaaaatta tccaaaagag aaaaagattt tattcaatat ggatttttat
     2521 tatttttgat atgtattact acttatttag tattaactac tttagatata aaactaatac
     2581 ctaaaattat aaggctagta aataagactt atatatttct tggcatattg acaattatag
     2641 tatatatgat aattgaatct attgtaactc atttgataat aaatgcagtt caaaaaacaa
     2701 aagtaaaatt tattggtatt aaaatggcta caatgggatt ttactataac ttagttactc
     2761 catttgcatc tgggagtcaa ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc
     2821 ttagtaaatc agtggctata gttactaata aaactgtagt atttcaaagt acagttactg
     2881 tattttgtgg aattttaatt ttgctaaata agtcattact gatggagcaa gtacattctg
     2941 tgaaagtctt gataactttt ggaatgatta tgaatgtaag tatgttgtta ggtggactac
     3001 taatagtatt tagtccaaaa caagtaaaaa tgatagcaga agttttgatt aattttttat
     3061 ctaaatttaa aacatttaaa tttttagatt caaaacgtga tagaataaat cattatattg
     3121 atgattataa ttattctata aaaatattta ttaaaaatgt aagaatctta gttttaagta
     3181 tattacttac tgtgttacaa ttaatcgcat atttcagtat agtttattgt gtgtataaag
     3241 cctcaaattt aagaggagtt tcatatgtac atataatgac attacaggtc tttttatata
     3301 tggctatatc accaattcca acaccaggaa atgttggagc taatgaggtt acatttttta
     3361 ctatgttctc caatataatt ccaaaagaat tattggggta ctctgtactc ttatatagtg
     3421 ggtttgtata ctattttata ttaatagttt caggtttatt tacatttgga acgcattata
     3481 cattaaattc ttggaaaaag gaagataaag taaattctaa tttagataag gttattgaaa
     3541 aattaaatta ataaaataat tagggaaatt ctatatataa atatggtgtt gaataagcta
     3601 tattatattg agatatttcc cttttttgat taaaattata gaaatttgaa ataataagta
     3661 ataaacagaa aaagtgatat tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat
     3721 gaaaaacaaa gtgagatttc taagataaaa caaagcaatg ataataaact tgaaatagct
     3781 caagaattac atgtaagtaa aaatataggg aatatgcttg gatatagaat agataagaaa
     3841 aaaatgaata gaccaagtta atgaaaatgt tgaaaatggt acattttaaa aaatatatat
     3901 aatgaaatgt tttattgtta aatttaaact tattttaatg gaggaaaaag tcgaaaaata
     3961 tctaaaaaga tatggatata aaaaatatta atatatatat actataaact tgacaaaatc
     4021 aaccttttaa taactagcaa aaaatagcaa aattatataa gcgttattta attgtcaaaa
     4081 atgaaagcta aattgcatgt cctaaaaaaa atgattgata acatttattt gtgtgctata
     4141 attatatagt aaaacaataa gtatattgca taatgtatgc aaatcgggag gagagacgga
     4201 catggaagca tctatacaca caattgccag caataattta ttgataattt ttgctattgt
     4261 aagtataact ggaataattt gtagtaagct tagtgaaata ataaaggttc cagatgtagt
     4321 gttatattta cttgtaggta ttttaatagg accatcattt ttaaaattta tagatattcg
     4381 tggatttcaa atagaaaatc aactaatact tacatttggt tctgcattta ttttatattt
     4441 aggtggaaaa gaaataagtt taaaagtatt gagaaatgtg aagatatctg tattcttatt
     4501 agctacttta ggagttgtga tatctgcttt tataatgcaa caagtaatag gatttacttt
     4561 tgggataagt gcaatgactg ctttactagc aggaactata atagcatcaa cagacccagc
     4621 cactcttgta cctatattta atcaggtaaa gataaaggat agggtaaaac aaactgtaat
     4681 aagtgaatca gcttttaatg atgcaacagg agctatacta acatcagcag tgttggctgt
     4741 aattttgtca aataaatttt cattaggtga aaatatttat gaactgggga caatggttat
     4801 tgtcggagtt ttagttggtt taattacagg aatactgctt ttgaaattag ttaatgataa
     4861 accttatgga atatttaaag attttgcacc aataatatct gttatttcag ttgtagttgc
     4921 atatgaatta tctacaaaat taggtggaag tggatatatg tcttgcttta ttgtaggtat
     4981 aataactggt aacaagaaaa actttaaaat ttggttaagt caaaaatctt atgatgcaga
     5041 tttttatgtt gctgaaactt taggtactat ttgtcgtatg gcaatattta taatattggg
     5101 tagccaagtt aatttggctc agttaagtaa atattttatg ccatctgtaa ttacagtatt
     5161 agtattaatg tttatagcaa gaccactttg cgttttagtt tgtacacttg tagacaggga
     5221 agcaaaatgg agtaaaagtg aaatattatt tatgatgtgg gtaagggaaa caggcgtaat
     5281 accagcagca ctttgtggta ttatttcagc aatgaaagtt cctggatacg aagtgatttc
     5341 atctgtagta ttcatgacta tactaattac attagtaata caaggaagta ctacaaaatt
     5401 agtagctaaa aaacttggat tattagaaga agaagtgata aatataagtg aaaaagtatc
     5461 aactttttaa agttttgctt taaaatagta cctgttaatg gctattttaa tattgtctaa
     5521 tgatgattat tgaaatagtc atttttttat gtataagtaa tttacagaat ttacatatta
     5581 gacatattta ttatcaaaat ttgttgaagc tatgatataa ttagttgtat ttaaaataag
     5641 aatagcaaat aagggaggaa atttatgttt acggttatgg atattgtaca gccagataca
     5701 gtagaagaag cgtactcaat tttaaataaa agaaaaacta accaagtaat tggtggaagt
     5761 gcttttttaa gaatggggaa aaaaagaata ggaacaggaa tagaattatc taaattaaat
     5821 ttagattata taaaagagta tgaagattac gttgagattg gttctatgac tacttttaga
     5881 acattagaaa caagttccat aataaaaaat aattttggaa gaataataga agattcagtt
     5941 aaaaatataa taggagttca gtttagaaat gtagtaacag ttggtgcaac agtattttct
     6001 aaatatggtt tttctgactt gatagtagca ttactttcat tagatacaga agtagaatta
     6061 tataatattg ggagaattag tcttgaagag tttctaaata gagattatga gaaagattta
     6121 cttattaaga tatacataaa gaagacaaat aagaatgctt cttataaatc acttagaaat
     6181 gcaaagagtg attatccaat tctaaatgta tctgtatcaa aacatatgga acaatttaag
     6241 ttatgtgtag gagcaagacc gcagaaagct acgattgcaa aacaagctag tgaatttcta
     6301 tctaacaatg aaatcaatga aattaatata gataaagcag tagaaatagc atcagaagaa
     6361 ctaacttttg gttctaacat gagagcatct agggagtata gaaaagctat gagtaaagta
     6421 cttttaaaaa gagctataat ggaggttatt taatgttagt aaagattaat ataaatggga
     6481 agaaaaaaag tgttgatata gaaccagaag aatatctggt tgatacttta agaaaagttg
     6541 gtaatttaag tgtgaaaaga ggttgtgaca caggctgttg tggtttatgt acagttttaa
     6601 ttgacaagaa acctacatta tcctgtgcaa cactgacatt gagagcttta gataaagaaa
     6661 taactacaat tgaaggttta gaaaatgaag ttaaagaatt tagtgaagtt ttggtaaaag
     6721 aaggagctga gcaatgtggt ttctgttctc ctggctttat acttactgta cttgcaatga
     6781 aggatgagct tgaaaatcct acagatgatg aaataaagca ttatcttaca ggaaatctat
     6841 gtagatgtac agggtatatg ggacagttaa gagctatcaa aacttatctg gaggtaaaat
     6901 agaacatgaa tatagttgga aaaggaatca agaaaataga tggaatgagt atcacaacag
     6961 gaaagcctgt atatacagat gatttatcag caaaagatgc tttggtggta aagatactta
     7021 gaagtcctca cgcttatgca aaaataaaag atatagagac atcaaaggca gaaatggttg
     7081 atggtgttga atgtgtttta acatataaag atgtaccaaa atctcgattt acacttgctg
     7141 gtcaaacata tccagagcca tctccatatg atagattaat tcttgaagat gttgtaagat
     7201 atgtaggtga cgaagtagct atagtggcag ctatagatga aaaaactgca attaaagcaa
     7261 tgaatcttat caaagtaaag tatgaagtac ttacacctgt tttggatttt gaagaggcaa
     7321 taggaaataa aactatggta catgatgaag atagacattg taactttgat ataggtatgc
     7381 aaagagaaaa aaatatagta tcccatcatg attatacaaa aggtgatgta gaaaaagtat
     7441 taagtaattg tgatgtagtg attgaagaaa cttattatac tcaagctcaa gcacatgcaa
     7501 tgatggagac gtatagggca tacaattatt tagaccatat gggaagattg gttgtagtaa
     7561 gttcaactca aataccattt catgtaaaaa gacatttatc tcgtgcacta gaaataccag
     7621 ctagcaaaat tagggttata aaacctagaa ttggtggcgg atttggtggt aaacaaactg
     7681 catgtgttga gattttttct gcaattgtta ctttaaaaac agggaaacca gctaaaataa
     7741 tttatgatag aaaagaaact tttaattgtt caacaagtag acatgcaatg agattaaaag
     7801 ttagactagg tgctacaaat gatggcataa taaaggcaat agatataaaa gcactttctg
     7861 atacaggagc atatggagag catgcttcta caactattgg tttggttgga gaaaaaactt
     7921 taccaatgta taataaagtt gaagcaacaa gatttattgc agatgtggta tatactaata
     7981 agatgccagc aggagcactt agagggtatg gagcatcaca aggatgtttt gctgttgaat
     8041 caactataaa tatgcttgct aataaattaa aaatggaccc aacagattta aggttaaaaa
     8101 atgtggtaac agaaggtgaa actacatttg catatggtaa aactttaaat agtgtcgatt
     8161 taataaaatg tataaataaa ggcaaagaac ttattggatg gaatgaaaaa tatccttgta
     8221 aacatcttga taatggaaaa attagaagtg tgggtatggc aattactatg caaggttctg
     8281 gtatacaagg tcttgataag tcttcagctg aaataaaatt gaatgactgt ggagattata
     8341 ctcttatgat aggttctact gatatgggta caggaagtga tacgatttta gctcaaatgg
     8401 ctgctgaggt tcttgataca agcattgaaa atataacagt tatatcagct gatactgact
     8461 tagttccata tgacccaggt tcatatgctt caagtactac ttatgtaaca ggtatggcag
     8521 tagttaaggc agctcaagac ttaagagaaa aaataatttc tgctggagca actaaactag
     8581 gtgttgagaa ggatgaagta gaatttgatg gtataaaatt aactagtaaa aaaggtagca
     8641 ctttattatt cgatatagca gttgattcta caattggtcc agatactaat caattaatag
     8701 gtcttgcttc tcatggtagc ccagtatctc ctcctccttt tatagcaggt tttgcagaaa
     8761 ctgaaattga cacagaaact ggaaaagttg atttagtaaa ttatgtagct gtagtagatt
     8821 gtggaacggt tataaataaa aatctagcta agatacaagt tgaaggtgga attgttcaag
     8881 ggattggaat ggcattattt gaagaagtta gatacacaga aaaaggaagc atggacacaa
     8941 atacatttat gcaatacaag attccagcta gatgtgatat tggaaatgtt aaagtagatt
     9001 ttgttgaaac ttatgaacca actggaccat ttggtgctaa atctgttgga gaagttgtta
     9061 taaatacacc tttaccagca atacaagaag ctgtgtttaa tggaattggt gtaagaataa
     9121 atacactccc tattacacct gaaaaagttt ttatggaaat gaataaataa ttatttatga
     9181 aaatttagac tagttttaca tgttaaacaa ccagttatgt catacctgtt gaaagatatt
     9241 taataagtag ttattataat attatgaatt ttacttataa aatacaaaat taaataaggg
     9301 gatgatgttt tggctggtaa tctaaataat atgagagcag taaataattt tagaggagat
     9361 aagaacattt tagaatgttt agtcagcttt gagggtcgtt caataagtca gagaaaagta
     9421 agggtatttt ttaaagaaaa acaaaatcaa atagaaattg attttgcaga agaggaaatt
     9481 tctaaattgg ttgaaaatgt tgttttaaat acatcatatc aagaaatgtt atatgatgaa
     9541 atagagaaac aactggaaat tgattgtata ggtacttgga tgatattatc taaattaaaa
     9601 gatggtagta gagttcactg attttaaaat aaattattag agcctaattt taaggctctt
     9661 ttttgctgtt tttaattaac aatagaaaat ttatctttag tttgtatata attttaatat
     9721 catatattct atatatataa aattgaattt gtatgtatta tgttgtataa tataaaatga
     9781 attaggaaaa tagttcgcta aaataatttg tttttataaa attactgttt aagtttatag
     9841 attttgtata aaataaagtt atgttattaa tttttttaga aataaaactt ttactaggag
     9901 acagatatga gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat
     9961 gcagttttaa cattatcatt gggatttata agttatggac caatacaatt tagggtagca
    10021 gagattatga tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt
    10081 tgttttttag caaatgtaat aggaccatat ggtgtacctg atataatttt tggaacatta
    10141 gccacattta taagtgcaag tatgatttat attactagaa agttaggtaa acagaataaa
    10201 agtactttag ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta
    10261 atgttaaatg tattctttgg gcttccatta attctatcta tgatacaagt aggttttgga
    10321 gaatttgtag tagtaacaat tgtaggagta ccatttttct gctttttaat gaaaaaatat
    10381 aaaaatgtca ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat
    10441 tattagtgaa aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat
    10501 atattaatga taagcaaaag tttacaataa aagagttgtc agaagaattt aatctttcag
    10561 ctaaaacaat acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag
    10621 aacaaggtag aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat
    10681 ttacagaaaa agaagttatg attatactat ttgcacttaa gtcacttcag atttataatt
    10741 ataagttaat ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa
    10801 gcagtataaa agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata
    10861 ctaatgatgg agattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat
    10921 ctctaatttt aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc
    10981 ctataggtat atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg
    11041 ataaaataat attagttcat atagataata taattgatat aaaaagaaca ggagaaagta
    11101 aaaaaataca tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa
    11161 atagttcaac tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag
    11221 aaggtgtttc taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg
    11281 gtagtggaat aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt
    11341 tattttttat tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt
    11401 atgataaggc caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa
    11461 tgttgaaatt aaataattat taaggagata tacgttataa tggaaaattt taataagaat
    11521 gctaaaataa ttggtattgg agctgaaggt attaatataa taaatgaagt agaagaaaaa
    11581 ataaaagcaa atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat
    11641 gttagaagtc ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa
    11701 catattagag atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct
    11761 ataggaatgg attgttcaga gaaggaaaat aaagaagatt tagagcttgg gagagagttt
    11821 aaaatcaata atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct
    11881 atttctgatt cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga
    11941 gataaaggaa ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata
    12001 gctgacattt tatttgatag gatggaatat attggtgagg aatttataag taagaaagga
    12061 attgtatttg ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt
    12121 aatattcaga gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa
    12181 aatttaaatg gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag
    12241 gagagtgtat atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt
    12301 ttatcaggaa acagacacac cacagatttt tagagaagaa agcataattg cacttggatt
    12361 tgatataaaa ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat
    12421 taatgagaat tgtaatgata aagattttat aaaaaagttt atgtcgatta aaaaaggaga
    12481 ttatttttta tataaagcat atcaaaatat agaagaaaaa gttaaatgta atgtgtctat
    12541 tggcatagtt gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac
    12601 actaccagta aaatggatgc ataattttga tacagtttca aattgtaaag tatatataag
    12661 tgaattttat aaattaaata ataatgatat tagctcatat ttagaaatta ttaagtcaca
    12721 agataagaaa ttattgagaa aaagaataga agaaagtgaa aataataaat ggtatttaaa
    12781 aaactattat tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc
    12841 cattaatcta gaacaattag aaagcctaaa aaatataaag acagatgatt atattttatt
    12901 gtataatagc gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt
    12961 atgtcaagtt atggaagggt tcgataaaga gaaagtaagt gctttggagg gtggatattt
    13021 ttttcctgtt agatggatag agtatgaaaa ggagaatata gacggaaatg catgggttat
    13081 atttgaaaat aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatggcatt
    13141 aaatactata ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt
    13201 ttacaatcta gtaagtgaaa ccagtctaag aaacagatac tatacaggtt acttagatta
    13261 taaaaacaat gtagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga
    13321 gggtcttaag tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat
    13381 atctatagaa gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga
    13441 aaaagatagc ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa
    13501 tatagaatat agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga
    13561 tgaaattaat agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga
    13621 tgataaaaga cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa
    13681 attttctcta cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat
    13741 agcactttta gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta
    13801 tgatttattg tctattgtag atggtataga tattagaaaa atgctatata caatcaataa
    13861 aaggatagag tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt
    13921 agaaaatatt aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga
    13981 gtatttttat tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga
    14041 aaaagataaa tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa
    14101 taatgcctca ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga
    14161 ggagctaaaa aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa
    14221 gggtaggtaa tggtgaaaat gagcttacag aaattgaata tgaaaaattg cttaagcatc
    14281 tagagaacaa taatgatgta ttaaaaagta atataataga tattaaatac aaaaaactta
    14341 gatttataaa ctatgtaggc atcatatgtt ttgaaaatgt aatacttgaa atacttccta
    14401 aattgtcatt aagtgataat cttgtaaaag atagagagat actattacag atgctttcta
    14461 tatgtaataa aattccaatt acaatgaatg aaaaaataag attgagtttg aaaaattata
    14521 atctcttaaa tttttttgtt atgtatttca ttgagagtat gcaaacacag atgaaaaaag
    14581 gtatttattt tgaatatata aataaaatag agaacttaaa tgttatgagg ggtaaaatat
    14641 tattaagtac atatgcaaaa gaaaaaggca tttctccaat gaaaataaga tgtgaatatg
    14701 atgagtatag tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt atatctattt
    14761 tatgtagaat aaatgacaat tcaattcaag gcaagattaa aaaaatttta agctattttc
    14821 aaaatgtaga tttaatttat atagatagaa aaaaactgtt ggattataaa ttttataaaa
    14881 acaatgatag atttaaagat tgttatttac tagctagact tctactttta aatttgtcta
    14941 tggataatag tcaaaataat caagaggcat tttctatatt atttgaaata aatactttgt
    15001 atgaagaata tataggaatt ttaatcaaaa gtatatggga caattcattt agagaaacat
    15061 atatacaaga taagagtaaa tttcttttga aaaatgaaca aactggtaag aaaaacttta
    15121 atttaagacc ggatatagtt ttatatgact taaaaaatga atatgaaatt ataattgaca
    15181 ctaaatggaa agctattgaa gtagattcaa atgtttttta taggtcaagt gatatatacc
    15241 agatgtatgc atatataaca gcatatgaaa atgcaaaaag atgtatatta ttatatcctt
    15301 gtattcaaaa agataagaat tatagttcct ggaaattatc agaatcattt aagggtaagt
    15361 ttatagaagc aaaaactgtg aggttagatg atataaaaaa cacaaaaaat gatttaaaaa
    15421 agataatttt taattataaa tttta
//
LOCUS       DAKBNN010000089        15183 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000089 DAKBNN010000000
VERSION     DAKBNN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.089"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(482..1225)
                     /locus_tag="OUB02_002243"
     CDS             complement(482..1225)
                     /locus_tag="OUB02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HCT8886000.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1391..3958
                     /locus_tag="OUB02_002244"
     CDS             1391..3958
                     /locus_tag="OUB02_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886001.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4188..4592
                     /locus_tag="OUB02_002245"
     CDS             4188..4592
                     /locus_tag="OUB02_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8886002.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4616..5113
                     /locus_tag="OUB02_002246"
     CDS             4616..5113
                     /locus_tag="OUB02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8886003.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5116..5517
                     /locus_tag="OUB02_002247"
     CDS             5116..5517
                     /locus_tag="OUB02_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCT8886004.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5535..6023
                     /locus_tag="OUB02_002248"
     CDS             5535..6023
                     /locus_tag="OUB02_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8886005.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6050..6805
                     /locus_tag="OUB02_002249"
     CDS             6050..6805
                     /locus_tag="OUB02_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8886006.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6805..7668
                     /locus_tag="OUB02_002250"
     CDS             6805..7668
                     /locus_tag="OUB02_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT8886007.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7715..8194
                     /locus_tag="OUB02_002251"
     CDS             7715..8194
                     /locus_tag="OUB02_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HCT8886008.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8259..9566
                     /locus_tag="OUB02_002252"
     CDS             8259..9566
                     /locus_tag="OUB02_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8886009.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9876..10016
                     /locus_tag="OUB02_002253"
     CDS             9876..10016
                     /locus_tag="OUB02_002253"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HCT8886010.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            10526..11620
                     /locus_tag="OUB02_002254"
     CDS             10526..11620
                     /locus_tag="OUB02_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886011.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11625..11825
                     /locus_tag="OUB02_002255"
     CDS             11625..11825
                     /locus_tag="OUB02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HCT8886012.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11825..12742
                     /locus_tag="OUB02_002256"
     CDS             11825..12742
                     /locus_tag="OUB02_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886013.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12739..13497
                     /locus_tag="OUB02_002257"
     CDS             12739..13497
                     /locus_tag="OUB02_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886014.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            13745..14818
                     /locus_tag="OUB02_002258"
     CDS             13745..14818
                     /locus_tag="OUB02_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HCT8886015.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14888..15151
                     /locus_tag="OUB02_002259"
     CDS             14888..15151
                     /locus_tag="OUB02_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886016.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
BASE COUNT         5863 a         1589 c         2799 g         4932 t
ORIGIN      
        1 ggggctagag tctggaaatt tttatttaaa gaatacacat aaaaatttct agactctcat
       61 catttaaaag aagtattcta aatttattat ttataattaa atattatctg aaaaaatggt
      121 atttttatac ctttgtttgt tatgcataat tttacttaga acataagctt tatataaaat
      181 aattaaaaat atacctaatt aaaataagta aaaaaagagc tatcatagaa caaatttaat
      241 ttgctcatgt gacagctcct ttttagatga tgctttttta gatttatact ataaggtata
      301 taaaactaag tttattaaaa atgttataat ttctattgct atcatagacc atatcaatcc
      361 atcaagttca taaaagtaat taagctttat taaatagctt ctttattata atttctttat
      421 ttcatataat ataaatagta ttaaaatttt tatgggagtt aaagtaagca tgtattatta
      481 cttagatatt tgtataatca acatctagta atttaccttt ttggtgaaaa tgattaataa
      541 gttttttagg ataattcatt attaattcat ctggaaaatt aatttccttt aagatatttt
      601 cagaatactc atagttacct atgtcaaaac aaatgtgagc atctgaagtc agtatgattt
      661 tagcaccata ttttttgcaa agtgtagcaa tatacttaca gttatccgag ctaccatttc
      721 ttgacacgcc tttaatagaa caattattta tttctattaa gatattcttt tctatagcta
      781 atttaatgat taattcatag tcgattgggt agtttgggtt tccaaggtgt cctaatattt
      841 caatattatc atgcttattt attgcattta ttaaagcttt agtattattc tctaatgtat
      901 ttggagaaaa cacagcttcg tgaaatgaca agataagata atctaaccga ggaattacaa
      961 aaggctctag gtctatgttt cctttaatat ctagtatatt tgcttcacag cctcttaaaa
     1021 ttataatgtt atttatgatt cttgggatat tttgaatgtt gtgaaagtac catttgtggg
     1081 gagaaccagg catcttagga ccatgttcag atgtacctag tatcttaatt ccattttctt
     1141 tacaataatt aatattttct agtaaagtgc tatagccatg accactgact atagagtgtg
     1201 tgtgtaaatc tgataaaaaa ttcatataaa ttcctcctct ataaaagatg agggtaacgt
     1261 aatttatatt ataaaacgaa taggaaagaa aatcaatagt tacataatta gacaaaaata
     1321 ttaaaatatt gtagaattaa gcttaataat aaatgtttaa cagtattact gcttataggg
     1381 gaggttaact atgacacaaa agaagctatc aaggaaagaa aaagttttaa tttgcttgaa
     1441 aaggttaaca gaagaacact tgaactttaa taatggcatt agatttggat ttgatgcaga
     1501 atatatagct actgaagtag gtataaatag aagtaatgct agtaaggaat taaatcaatt
     1561 aataaaagaa agtattatta taaagataaa gggtaagcct gttcagtact tatataaaga
     1621 aagtattgag agtattttag gaaagaaact aaataaaggg atatttgata atattaactc
     1681 tataaacgaa tatagtgaaa atgattctat caaagatgag tatattttag agagtataaa
     1741 aaaagagaac aatattttta atttagtagg atgtgatgga agtttaaggt cccaaataga
     1801 acaggctaaa gcagcagtaa tatatccacc taaggggttg agtacattaa taataggacc
     1861 tacaggagtt ggtaaatcta tatttgcaga atatatgtat aagtatagtt taactataaa
     1921 agaagatagt gataatgcac catttatcat atttaactgt gctgattatt ctgataatca
     1981 gcaattatta ttagcgcagc tttttggata tgtaaaagga gcatttactg gagctgataa
     2041 agataagtat ggtattgtac atgaagctaa taatggagta ttgtttttag atgaagttca
     2101 tagattatct gcagagggac aagaaatgct atttctgctt atggataaag ggatatatag
     2161 aaggttagga gaggctaata aggtacacga gtgtagagtt atgataattg cagccactac
     2221 agaagaccca gaaacagcaa tgttggaaac gtttttaaga agaatacctg taactataaa
     2281 gattccgtct ttagaggaaa gaggctttca agagagaatg aagcttatat gttatttttt
     2341 taaagaagag agtgttagga ttggtgttaa gttaaaggtt tcaaaagaag ttattaaggc
     2401 atttatttta tataagtgta agggaaatat aggccaatta aaaagcgata tacaattaat
     2461 ttgtgcaaga gcttttttag attatatgac ctataaaagg gaatgtgtat atgtaaggct
     2521 ctctcttcta ccaaataata ttagagaatc attatatgga aataatagaa aagaggagtt
     2581 tgtacaaaat ttcagtttta ttggaaaaga tgatattatt tttttaccag aagaaaagga
     2641 ttttgaaagt gaaaatttat taatagaaaa taaaaaatat gatttagatt tttatggaat
     2701 gataaaagaa acttggaata aacttcaaca agaaggtatt aaagagtcac aaattagaag
     2761 tgttatagat ggagacatac agaagtattc ctataattta atgaatactt ttatatataa
     2821 tagtagtaat caaacagctt ataacaacat tgttaatgat tcaattgcaa ctactgtaaa
     2881 atatatttta caaaagcatg ataaatggag caaaagagaa ggattagata aattaataaa
     2941 agctatagct ttacatattc agaatttaat tgaaagaata aagattggaa atatagtaaa
     3001 gcatcctaat aaagatgaaa ttatgaagga aagagcatat gaatataaca tagcaaaaga
     3061 aatattatct aacatgtcat taatgtatgg agtagagttt ccagaagatg aagcaatttt
     3121 tttagcaaca tttctttatt tatcgtatgt gggattaaat gaggagagta tagcaatatt
     3181 agtcataatg catggtgaat ctacagcatc tagcatggtg aatgtagcca atactttact
     3241 ggattgtaac catgcagtgg caattaatat gggattagaa gatagagttc aggatatttt
     3301 agtacaagct gtagaaatag ccaataaaat tgataaagga aaaggaattt taattttaac
     3361 tgatatgggg tctattttaa catttgctaa agttattcgt gatgctacag gaaaagaagt
     3421 aaaagcaata gatatggtaa gtacacctat tgtaatagaa gcaactagaa aggctctaac
     3481 tccagaaatg acattagaga aattatattt taatattata gaatctataa agaaacatta
     3541 tggagatatg gaagttacat atgctgaaaa agataatggt ggtagatatt ttgatagatt
     3601 actaattgat aatattagta aaactttgac ttttttaaat ggggaaaagg cttattttat
     3661 attaaaagaa gtaattaata gaattagtga acactattct ttagtaattc aagatgaact
     3721 tttagtaaag tttatatttc attgttcatg tatgattgaa agagtaataa taaaagagtc
     3781 tttagtttat aaatcatatg aagaaagaat tagtagaaat aaagaattat atttagttat
     3841 aaaagaaagt tttaagctag tagaagagac attcgatata attatttcag atatggaata
     3901 tgcaaatata ttagatatat ttgaatcaca atatgataca gataaattca aaagttgata
     3961 caaaatttaa aaatagccaa aaggctatct tttttttact ttttttgata cagatttgat
     4021 acaggttgaa aaaatctttt gatacatatc gaaataataa gtttttaaaa aaaattgagt
     4081 aaaaagtttt gattaaatcc tgtaaaatag ccatttgtta ttgatacaca taagttggca
     4141 cggcaattgc ttatataaaa agtgacaaga aaagtgaggt gaaattggtg ataggaataa
     4201 tagttatgac tcatgggagt tttagtgaag aaattataaa aagctgtgaa cttattgcag
     4261 gaccagcaga aagaacagca gcaattaaac taaataggaa tgataatatt gaagacttaa
     4321 ataaaaattt tgtagaaaag ttaaatgaat tagatgaagg tgacggagtt ttagtattag
     4381 ctgacctatt aggaggttcg ccaagtaatg ttgcaagttt aaaccttaaa aaaggtggca
     4441 agtttcatgc acttactgga gtaaatcttc caatgctact tgaagcttta attaatagag
     4501 agggaaaaag tttagaagag ttagcagaag catgtattga agcaggacaa actggtataa
     4561 acaatattaa taaagttctt agctcaatgt aatagaaaaa ggagtttgat taaaaatgtc
     4621 tttagtaaaa tattgcagat gtgatgatag attaatacat ggtcaagtta tatataaatg
     4681 ggttaagcat ctaggtgtaa aaaagatagt agtagtagat gatgaaacaa ccaatgatgt
     4741 tattgctaaa ggtttgataa aaatggcagc accaaagaat attgatttat caatattaac
     4801 tgtaagtgaa tcaaggagat atttttataa taatcaagct gatgataacg tttttgtatt
     4861 aattaaaaat ttagatactg caaatagaat gatagaagaa gggattaata tcaaaaaact
     4921 tattgttgga agaataccta caggaatagg aaaaaagaaa atatctcaaa atgtatacat
     4981 taataaaaaa gagtttcttt taatagatga atttattaaa aagaatatta atgtatcaat
     5041 tcaaatggta cctgatgaag aagaagtaga attaaaccaa aatattaatc gctataaagg
     5101 agagtttatt tagcaatgaa taaatataag gtaatcatac tgtgccatgg aacttgggga
     5161 aaagtcttag tagaagaaac aaataaaaac tttggattgt caaatcaata tgaagtttta
     5221 tcactaagtt ctgaaaaaga tgtttctgta tttatgaaag aggttgaaga agtagtagat
     5281 aaagaagatg tgaaaattat aatatcagat ttatatggag gatctacatc aagtgttgca
     5341 atagctgttg ctataagaag aggaatagat gcaataaatg gacttagctt acagacaatt
     5401 ttaatcgtag atgaagaatt aagcaaagaa gataaattac atacattacc agaaagaatc
     5461 gtcaaaaaaa ataataacct ttgtgtggat ttaattaaag aatttaaaaa aatataaatt
     5521 atgaggtgaa tataatggca aaaataacat tagtaagagt agaccacaga ttaattcatg
     5581 ggcaagttat aacaaaatgg gtaaaaatcg ctcaagctca aaaaataata atagttgatg
     5641 acttcttagg tcaagatgaa tttatggctg acatatataa aatggcagca ccatcaggag
     5701 tagaagtagt tattttaaca gcagaagatg cgggacaagc attccaaaac aacactttag
     5761 gagataaaaa tatatttatt ctatttaaaa atgttgatat ggcaaataag gcttataaag
     5821 ctggacttaa atatgaaaag attcaattag gaggtattcc aaatgaagca ggaaagaaaa
     5881 tggtgtttac agctgtttca ttaggaaatg aggatgtaga acaacttaat gaacttaatg
     5941 aaaatggggt tgaaatagtc ctacaagtta taccagagga atcatctatg acatatgaaa
     6001 atgcgttaaa gaaatttaaa taaaaaatat aaattaggag gaattataaa tggaatctgt
     6061 tttgatgttg gcaattgtaa caggattatg gtattggttt gcagcaggtc ttgcagggta
     6121 tacccttttc tcaacactaa agtctccttt atttataggt tttagccttg gattactttg
     6181 gggtgatgta acaactggta tgattgttgg tgcaagtata gaaatggttt atctaggaat
     6241 ggtcgcagca gggggaaata ttccatcaga taaatgtcta gctgctctta ttgcaatacc
     6301 agtagcactt caaactggtg ttaatgcaga agttgcagta tctattgcgg tacctttagg
     6361 tgttatagga gtattagtta ataacttaag aagaactggt aatgctgtat tggttcataa
     6421 ggcagataag tatgctgaag aaggtaatac aaaaggcatc tggagatgtg caacacttta
     6481 ttctttaata tttgggtttg ttttaaggtt tccaatagtt tttgtatgta acttttttgg
     6541 agcagattta gttcaatcat tattagatgt tataccacaa tggttaatga atggattaac
     6601 agtaatgggc ggtatattac ctgcattagg ttttgcaaca acaatattta ctataggtaa
     6661 aaataaattt ttaccaatgt ttattatagg attttttatg gttcaatatt ttgaaatatc
     6721 aattactgca gctgcaatat ttggtgtttg tatagctctt ttaattacct ttatgaaaga
     6781 agacaaaagg gtaggtgaag tataatgcaa gaaataaaaa ataaagaagt taaaaatgga
     6841 caaggtccaa ctgaagttaa taagacaaga gtacttacaa agaaagatgt aacaaaaaca
     6901 tatctaagat ggtggtggac agcagaatta tccaactctt ttgaaagaat gcaagcatta
     6961 gcagtttgtg caagtttcac tccggcttta gaaaagttat ataagagaaa agaagattta
     7021 gtagatgcgt taaagagaca cttacaattt tttaatactc aagctatatg gggaggatta
     7081 attcacggta cagttttagc tatggaagaa gagaaagcta ctgaaggaaa aatcccagga
     7141 gaagttattt caggtgttaa gaatggtctt atgggacctt tggctggaat tggagatact
     7201 ttagattttg gtacatttca aacaatattc ttagctttag gtgcttcatt tggagcagaa
     7261 ggaagtgtta taggtgcatt tttcccaata atgtttagta tattactatt ttgcgaaggt
     7321 tattacttat ttcatttagg atattcttta ggtagagatt caattaagaa aattctatca
     7381 ggtggtattg tcaataagat aattgatggt gcttcaattc taggaatgtt tatgatggga
     7441 gcattatctg caactacagt taagctttca actccgctat catttgatat tggtggtaaa
     7501 gcaattgttg ttcaagatac attaaatatg attgctccag gtttattacc acttggtgtt
     7561 gtattcttcg tatattgggg aatgaaatac aagaagtgga caataactaa gttattagta
     7621 attttagttg tattagcatt ggtagggtca ttcattggaa tattctaatt cacagtgatt
     7681 aaaatataga atttaatttt aggaggagaa agaaatgtca gaaaaaatgt atacagaaaa
     7741 ggaatattta gaaggaatta gaggagcaac aggagaaaga gcagtatggt tctatcttct
     7801 tatgaaagaa gctgaaaaat taggagctaa tccagatgat atatgtaaag aagcaatata
     7861 tggatttggt aaaatgagag gtcaaaaata taacgtagca gatactccag gaaaaatggc
     7921 agaaatgtta tataacagta aaggacaaaa agtgtttgaa atggaattag tagaaaatac
     7981 agatgagaat ggcgttttaa aattccatca ctgtccatta gatgctgctt ggaaagaata
     8041 tggattaact aaagaagaaa gaaaagaaat ttgtagatta gcttgttatg gggattatgg
     8101 aagagttgac tgcgcacaag gtgttaaatt agattttgct caaaaatgtg ctcatgatga
     8161 tgaagtttgt gaattagtat ttacaaaaaa ataattttta atgctaaaag cctgttcttt
     8221 aaggacaggc tttaaaaaga tagaaagtag gttgatttat ggatataagc agaagtgtag
     8281 ataatagttt aaatagaacc atagaatttc taaaagaatt gataaaaatt gattcacaac
     8341 aaggtgaacc tatttcacaa tgtccttttg gtataggacc taaaaaatct ttagataaaa
     8401 ctttagatta ctgtgctagt ttaggtttta gtgttaaaaa tattgataat tatattggat
     8461 atgcagaaat tggagaggga gaggaattaa tagggattcc aatgcattta gatatagttc
     8521 caccaggaga ggggtggagt gtagacccat tttcgggagc agtaattgac aatattattt
     8581 atggaagagg tgtcatagat aataagggtg ctgtatctat gcttatacat gtattgaaaa
     8641 acatagagga tatgtatcct acaattaata aaagaataag attaattttt ggtacaaatg
     8701 aggagacagg tatgaaatgt atcaaatatt acttagataa aggtgaagaa attccaagta
     8761 tgggatttac tccagatgct atgtatccag tggttaatgg agaaaaagga agggttcata
     8821 taaggataga aaaagaaatt aaaattgaca aaagtaaacc ttacataata gtaaggggag
     8881 gaactaaaga aaatgttgtt ccatctcatt gtactgcaaa aattattaat ggaattattt
     8941 cagagttaac tactaaagga gtagctgtac atgcaagtaa tcctgaaaag ggagaaaatg
     9001 ctatatctaa aatggtaata aagatagttg aggataatat ggattttcag catagagaag
     9061 atatagaatt agtttcaaaa tacctttgta gtgattatta tggagatgca ttggggatta
     9121 atcaatatga tgaggtattc aaaaacacta ccttaaattt aggaatatta aaagtaaatg
     9181 aggaaaaaat cgtatgtgag ttagatataa ggtatggtaa aaatatagtt ttaaataaca
     9241 taatagatag attcaaaaag gttttttgta atggttggaa aatagaggtt attgctcata
     9301 aagacttaca ttatgtggat gagagtaatt tagtacttaa aaaattatta gaagcctatg
     9361 aagaagtaac agatgaaaat ggatatacaa ttgctatggg tggtggcaca tatgcaagtt
     9421 ggtttaaaga tatggtggcg tttggaccta agttcttagc gtataaaact ggtggtcatg
     9481 gagtagatga aagagtacca ataaatcata tacgaaagaa tatggagata tatactttgg
     9541 ctttaataaa actattagaa ttgtaaaaga agaagagact ttatgtgcaa atttacagat
     9601 atcttaaaaa gagagataaa acgagttcaa ccttatttaa atgttcaaca ccatcaaatg
     9661 gggctgtctc aaaatagaga taagcctctt acttattgtt gaaatgatga aaatatattt
     9721 tacttcatac aaaaaagagt gccttatgaa gtaaaaagtt aattttgaga caccctcatt
     9781 tgatgatgtt gaagagaaaa taagtatgaa attattaaaa aagatgattt taaaaaagtt
     9841 aaggttgaaa agaaaagtta attaaatgga attagataaa tggagaagga tgtatactat
     9901 ctaataaaat aacaaaagag tgttataaag ttggttatat gattagagaa aagtctattg
     9961 atacttatcc agatagtgga tggggattta tgacttggga tgaaaatgga gaataataaa
    10021 aattatagtt tatacagttt aataagatat atgtacaagc agttaatcta aatgattgac
    10081 tgcttttctt ttatagattt ttatataata gacaaatacc aatacatgat agttgcctgt
    10141 tataattaga ggaataaata cgaataataa ctttataaaa gaattaaatg gaaaatcgaa
    10201 gagttatatt cagtatttta aaaagaatat gaaatgagaa atgaaaataa ggtgtttata
    10261 ttaaaagagg ttttacacaa aatatttaaa aacgtgataa ataaaaactg tatttagata
    10321 gattaactat tagttttata taatatggat tttatttaca aagtttacat attttacaaa
    10381 agagattaaa gataaattta aatatatatt ttatatactt gtttatttgt ttctctgtgg
    10441 ttgatttttt cgtcaaaagt caacttcaaa tcgcttgtta gctttacatg tttaaggata
    10501 taattataca taaggaggga ttgacatgaa tgaattaaac atcgcaaaaa cgctgatttt
    10561 aaagcgtaaa gaaaaaggaa ttacacaaga tgaacttgca aattatatag gcgtttctaa
    10621 agcttctgta tctaaatggg aaactggaca aagctatcct gacataacct ttttaccgca
    10681 actcgcgaca tatttcaaca ttaccgttga tgagttaata tgttatgaac ctcaaatgat
    10741 gaaggaagat attaataaac tgtataataa actgtgcaaa gactttacgg ttaaaccatt
    10801 tgatgaagtc atggatgaaa taagagaaat cataaaaaaa tattattcat gttttccttt
    10861 gatttttcgg atgggtcttt taatagttaa tcactatgat attgtagatg aaaaaaagcg
    10921 tgaactatta attagtgaag cattagaaat atttattcgt atacaggaaa cttgtaatga
    10981 catagatatt tgccgtcaag caaaaagtat ggaggcaact tgttatatat tgttaaatca
    11041 acccatacaa gtgattgatt tattgcaaaa cagcaatttt cccatgataa atgaatctat
    11101 acttcttgca caggggcaaa tgatgaatgg ccaaatggat gaagcaagag aaacatttca
    11161 acttggtgca tatcagaacc ttatatcatt agtccaaaac ctagtgggta ttttgcaaaa
    11221 tgcagataaa ttacaaatga aggaaataga aagacgtatt ctagcaattt cagatatatt
    11281 tgagcttgat acattatctc ctgctattat gctttcttca tatttaacac aggcacaaat
    11341 taatttaata catggtgata atgaaggagc tattaaatca ttacgaaaat atgtagattt
    11401 agcaacacgt gatatatacc caattataat acatggggat gactttttta ataaacttga
    11461 cagatggatt tctgaaattg gaacaggtat tacacgtgat gatacaatcg taaaagcagg
    11521 tattgttgct gctattaaaa ataatccaat gttttctgta ttaagtgaaa acaaagaata
    11581 caaatttctt atagaaaaat tatctttatt ggaggaataa gattatggaa aatttgatgg
    11641 aatacttacc acttttggtt cctgttatta ttttagattt aatacttata attactgcac
    11701 ttgttcatgt gttgagacat ccaaattata aaattggaaa taaggcaata tggattattg
    11761 tagtactgtt tattagttta ataggaccaa ttttgtattt tacaatagga agaggtgaag
    11821 aatgatgaat attgtctcaa taaatggatt atcaaaaggt tttggtaatc gaaagataat
    11881 agataattta aattttactg taccagaagg gtcagtcttt gggtttgtag gtaagaatgg
    11941 tgcaggtaag actacaacaa tgaaaatggt acttgggtta cttaaaccag atagtggaac
    12001 tatagatgtt tgtggtgaga aagtgaccta tgggaaaaca agttctaatc gtcatgtagg
    12061 ttatctgcct gatgttccag agttttacaa ttatatgaga ccacttgaat atctttctct
    12121 ttgtggtgaa attacgggac tttcaaaaaa agaaatacag ataaggagtg aagagttgtt
    12181 atcacttgtg ggtcttagaa atgaaaagag acgaataggt gggttttctc gtggaatgaa
    12241 gcaacgactt ggtatagctc aagctttgct ttcacgcccc aaattactta tttgtgatga
    12301 accaacaagt gcacttgacc ctgttggtcg aaaagaaata ttagatatta tgcttaaaat
    12361 caaagactca acaactgtaa tattttcaac acatatacta tcagatgttg aaagaatctg
    12421 tgaccatgtt gctatattaa acaaaggaag cattgccctt agtggtacac tttcagaaat
    12481 aaaaagtatg cacggaaagg atagactttt actggagttt gcaagtaatg atgaaattca
    12541 aaagtttaag tctagtgatg gtattaaatc cttactaaaa gattcagaag aaactaatat
    12601 ggaaattgtt ttgcatggta aagatataaa agcaatacaa aaaacagtta tatctacact
    12661 cgcagaaatg aatctttgtc cagtaaagat ggaactaata gaattatcac ttgaaaattt
    12721 atttttggag gtagtaaagt gagagaatac atagcattta caaaaaaaga atttaaagag
    12781 aatttaagaa attataaatt atttagttta attattttat ttctaatatt tggtataagt
    12841 agtcctattt ctgcaaaatt tatgccagac ttgattgctc attttgcacc gactttaaaa
    12901 gttactgctg caccaactgc tttagattct tggacacaat tttttagcaa tatctctggt
    12961 cttggtatga gtttaactct aattattttt tgtaatattt tatcaaatga atattctaaa
    13021 gggacacttg ttattatgct gactaaaggt ttatcacgtt catctatagt attgtctaaa
    13081 ttctctgtta cggtgattat tatgacaatt ggtttttggt tgagtttttt atttgcttat
    13141 ggatatacaa tgtatttttg gccaactgca aatcttaatc atattatatt ttcagcattt
    13201 aatttatggc ttataggaat tatgtatatt agtgtattaa ttcttgggtg tgtactattt
    13261 agaccagcat ttgccagtgt tttacttgta ttggtggcaa ctcttttatt gagtttaatt
    13321 tctattccta aacagattgc accttatact cctaatttct tgatgtcaaa aaatatagac
    13381 ttgatttcag ggaaagtggc aatgccagaa tttattattc caattattat aacagttctt
    13441 atttctgtag tatgtttact agcagctgtt attttattta ataaaaaacc agtttaataa
    13501 tacctttatc acaaggtaaa gtaatcttat gttgttaaaa actacaccta aaaatcaaaa
    13561 agacttggac ttaagtttca agtctttttg atttttaata tatagaatta agtgtagaaa
    13621 ataatacaaa taatattatt tgtatcaaat aaatataaga atatttaatt atgttaaaat
    13681 atactaaaaa ttactttaaa agtgataaaa ttagtataag taaatatttt aaaggggtgt
    13741 agatatggaa aagtcaaaag tatatttttg tgatttatac tcaaattcgc aaaataaaaa
    13801 cgtaccaaac aatgtaagaa gattatttga tgaagcagga ttcaaagatt taatagaaaa
    13861 aaatgaccaa gtcgcaatta aacttcactt tggagaaaag ggaaatacta catatatgag
    13921 tcctgtggca gttagacaag tggttgataa ggttaaagac tgcgaaggaa agccattttt
    13981 aacagataca aatacactgt atacagggag tagaacaaat tctgtagacc atcttacaac
    14041 tgctatagaa aatggatttg cttatgcagt tgtaaatgca ccagtaataa ttgcagatgg
    14101 attatatagt agaaattatg agaatgtaaa aatagacaaa aaacattttg aaagtgtaaa
    14161 aataggtgga gaaatttata attcttctgc aatgatagta atgagtcatt ttaaaggtca
    14221 tgaagcagca ggttttggag gagcattaaa aaacttagct atgggatgtg caagtgcagc
    14281 aggaaaacaa atgcaacatt ctgatgttac tccagtagta aaggaaaaga agtgtgtagg
    14341 ttgtggaaag tgtgttaatt cttgtcctac aaaagctata agtatagtgg ataaaaaagc
    14401 agttatagat tctgatgtat gttatggatg tggagaatgt ccaactgtct gtccaactag
    14461 agcagtaact atacaatggg aaagtgactc agatgtgttt gtcgaaaaga tggcagaata
    14521 cgcttatgga gcagtatcaa ataaaaaaga caaggtaggg tatataactt ttgttatgaa
    14581 tgtaactcca ctttgtgatt gtgtaccttg gtcaggtaga ccaattgccc atgatatagg
    14641 aatattagca tcaacagacc ctgtagctat tgaacaagca tgctatgatt tgatttgcaa
    14701 ggaaatgggt catgatgttt tcaaacatga acatcctcat gtaaatggaa ctagaataat
    14761 agactatgca tgtgaaatgg gtatgggaag taaagaatat gaattaataa aattataaga
    14821 atagtagcac ttaaatgcat ataattattg taagagaaat ctaaaagtat caaaggagta
    14881 tgcatttatg aaaatttact atgagaagga aaacagcaag aaggctaatg taaacaatat
    14941 tccaagtgta tttgcgttta attttctaag aaactttttg actagcaaag agtatgagga
    15001 aactagaaaa gaatatttta taaataatct aacaaaaagt caggttaatc aagccatgaa
    15061 ggatttaaaa tggctattta gagaatatga aggcttagag gttattacaa tggaaagctt
    15121 ggatggagtt aagacaagga tagttttata aaccagtaag aattatattc taataaaaaa
    15181 tac
//
LOCUS       DAKBNN010000090        14957 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000090 DAKBNN010000000
VERSION     DAKBNN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.090"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            138..695
                     /locus_tag="OUB02_002260"
     CDS             138..695
                     /locus_tag="OUB02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8886017.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            complement(1045..1407)
                     /locus_tag="OUB02_002261"
     CDS             complement(1045..1407)
                     /locus_tag="OUB02_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886018.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(1841..2650)
                     /locus_tag="OUB02_002262"
     CDS             complement(1841..2650)
                     /locus_tag="OUB02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8886019.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            2742..3365
                     /locus_tag="OUB02_002263"
     CDS             2742..3365
                     /locus_tag="OUB02_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HCT8886020.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            3362..4687
                     /locus_tag="OUB02_002264"
     CDS             3362..4687
                     /locus_tag="OUB02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8886021.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            4708..5247
                     /locus_tag="OUB02_002265"
     CDS             4708..5247
                     /locus_tag="OUB02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8886022.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLVIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREISFHLWKK"
     gene            5266..6657
                     /locus_tag="OUB02_002266"
     CDS             5266..6657
                     /locus_tag="OUB02_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HCT8886023.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFADYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVETRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            6672..7763
                     /gene="ytvI"
                     /locus_tag="OUB02_002267"
     CDS             6672..7763
                     /gene="ytvI"
                     /locus_tag="OUB02_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HCT8886024.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFAIINISYAGIIALFIALLDILPVLGTGSILI
                     PWGIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGILLLIAFLKKLNDKEIIHILH"
     misc_binding    8067..8280
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8534..9568
                     /locus_tag="OUB02_002268"
     CDS             8534..9568
                     /locus_tag="OUB02_002268"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCT8886025.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVRAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            9609..10826
                     /gene="argJ"
                     /locus_tag="OUB02_002269"
     CDS             9609..10826
                     /gene="argJ"
                     /locus_tag="OUB02_002269"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCT8886026.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            10841..11701
                     /gene="argB"
                     /locus_tag="OUB02_002270"
     CDS             10841..11701
                     /gene="argB"
                     /locus_tag="OUB02_002270"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCT8886027.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            11731..12918
                     /locus_tag="OUB02_002271"
     CDS             11731..12918
                     /locus_tag="OUB02_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCT8886028.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLQIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            13027..14049
                     /gene="argF"
                     /locus_tag="OUB02_002272"
     CDS             13027..14049
                     /gene="argF"
                     /locus_tag="OUB02_002272"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCT8886029.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            14176..14793
                     /locus_tag="OUB02_002273"
     CDS             14176..14793
                     /locus_tag="OUB02_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HCT8886030.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         5595 a         1638 c         2695 g         5029 t
ORIGIN      
        1 gtattatatt aaattataat taaactgtca agattttata atagtataaa gaattttata
       61 aattacaata attgacgaaa ctaattagat tagctaaact aataaagtaa ttaattttaa
      121 atgaaagagg gaaaatcatg cgtgttagta gagaaatagt tatacaagct acatcagata
      181 tagttaataa agatgggctt aacaaagttt ctttaaaagt tattgcagaa aagctaggaa
      241 tacgtacacc atctttgtac aatcatatag aaagtctaga tgatttatta cttgaagtag
      301 ctcataaagg aatgagagag atgaatagtc aaatgataaa ggcggctatt ggaaattttg
      361 gagatacagc tataaagtgt ataagtatag catactttaa ttttataatt tctaatcctg
      421 gtgtttatga aacaattcag tgggctactt ggcatggaaa taatgaaaca ttagaaatct
      481 ttgatgaata taaatcctta ttagaaaaac ttattctttc ttgtaatcta aatacaaaga
      541 atacaggtga agtattaaac ttaataatga gtgtacttca tggttattcg actttgcaac
      601 ttgggaaatc acttttgagt aaagaagaag ctataaaagg tttagtcgat tctattgata
      661 ctgtgttatt aggtattcat aaaaaatatg attaaggagg taaataaatt attttaactt
      721 atttattgaa cacttcatta cgtgggatgt aaaataaaat tttatcattt ttatgaaagg
      781 aaaatagtat atttcaagaa tatttacttt ctgaattggt aaaccctatt tttactgttg
      841 ttgttgataa tgaaaaggta gctgtaaaat tccttgataa tcagacaaaa gcgacacagg
      901 tatcatagta taaattgata tttcataagg acagtaattg gaaaattatt ggataggtta
      961 tttgattata taaaaatgat tatataaaaa aagaaaagac cttagctaac aacacatatc
     1021 aatttatacc ttgaagctgt ggttttattt ttcaaattgt ttgcggattt ctctgtcttt
     1081 acggattaca gtttttgctg attttagcag ttcaactaaa gaacggattt catattcatc
     1141 acaatctgag aaaatatctt tggcttcctt ttcaaaaatt actttggaag aaagtacatt
     1201 atcacataag agagtatcga ctgatacaga aagtgcattg gcaatcgcaa tcaaggttgg
     1261 caggctgact ttcgtttttc ctgtttccac gttgctcata tgggatggtg tcatgttgat
     1321 aatgtttgca acggattctt gtgttatacc ttttttaatt cttgcaattt taattcgctg
     1381 tccgattgct ttataatcaa tttccatagt ttttgttctc ctttcattct tttaaatatt
     1441 gtaactattt acagagcata atataatact ctatataaat gaataattta tataatatat
     1501 atttaagaaa aaaatttgca ctacttttaa gacaactaca aaagaaatgg aggcataaag
     1561 cctacatgtt aagattttat aaaaagatag taaaaaactg attaaatggt taacgaaaat
     1621 aaaaggctat ttctgtatct gaaattcatg aggaaatgca gtttgcattg aggaaacaaa
     1681 taatagcaat gaacctgaat ttctacaaaa atttggaaat aagataccaa ctttagaaga
     1741 taaaaaagga cattttaagc aaattattat agacaatacg agatattaaa aaaggcgtgt
     1801 atttctcatg tgatttgaga tatacacgct ttttatattt tcaaataacc tgcatagaaa
     1861 ttttttgaat gtagttttcg gcgttatgtt ctgataaata atcaattaaa tcttcttcat
     1921 aaatatttcc aacaatatga tacccttgtt cttctaactg atttttcata ttttgataag
     1981 ttttatataa actgtcataa ctcccctggt gatacataac tgcataattt ccctttggtt
     2041 taataaaaag gcgctcatca tctactttat ttttaataag gctataataa tagctctctc
     2101 taaaaattcc ctgttttata tttttttcaa gaacaatttc tcctacatga aaattataat
     2161 catgtaataa acaatattgt aagtgatttc caataccttg taacattgtt ttttcatctg
     2221 ttagtacagc ttcaggaatt ttagttgcaa tcagatattc ttcctcacaa tttacaattt
     2281 cgattttttc tgtgtttaca ttgagatgta tttccattgt tgaaatgaca tttctcaaca
     2341 tagaacacat tttttcaatc ttcttcactt cttcttgaag atccattagc tttctgtgaa
     2401 gtacattaag aaaggtattc gtatctaaat cattgagata ttgttttatt tcttctaaag
     2461 gtgtacctgc tttttttagc acagaaataa tattaaattt ttcaaattgg tcaattgtat
     2521 aataccgata gccattttca tgggtaatac tcggttttaa taattcaatt tcatcataat
     2581 ggtaaagtgt atgttttttt accccacaca attctgcaaa ttcacttgtc ttatataatt
     2641 ttttaatcat ttttaaatct cctattgact ttagggttac cagatacttt aggataacat
     2701 agaaagcttt tatattcaat gaaatgtgag gaaaagatat tatgaaaatt attacaatta
     2761 gccgagagtt tggaagtggt ggacgagaac ttggaaaaca acttgctgat attttaaatt
     2821 ttgactacta cgataaagaa attatcactg ctatagctag aaagaaaaaa attgacgaaa
     2881 actatgtttc ctacatatta gaaaatcatg gttggagaaa cgttccttta acaatgtgta
     2941 cttcttttac taatattttg tctatgcaat ccatgaagac agatttgtta atagaacaat
     3001 caaatgttat caatcagatt gccagtacag gtaaggattg tgttattgtt ggtagaaatg
     3061 cagatatatt attaaagaag taccagccat ttaacgtttt tgtttgtgca aatatgaact
     3121 ttaaaataca acgctgtatt gaaaaaaagt taaacaatga acatatttca aaaaaagaat
     3181 tagaaagaaa gataagagag attgataaaa atcgagcaag atcaagagaa ttttttacga
     3241 gctccttatg gggaaatcaa acttcctatc atattatagt taatacatct gaatggaata
     3301 taaaacatct ttcttatgca attgctaact tttcagagta ttggtttagg aggtatgaac
     3361 aatgatacag ttgtcagata attttaatta taaacgctta ttaaaattta catttcctac
     3421 aattattatg ctggtaataa gttctattta tggggttgtt gatggatatt ttgtttcaaa
     3481 ttttgtaggg aaaactgctt ttactgctgt caattttatt atgccatttt tgctaatctt
     3541 agggtgtgtt ggctttcttt ttggaacagg tggtggagct ttaattgcta aaacaatggg
     3601 tgaaggaaaa aaagaagaag caaacgaaca gttttcgtta ctcattgtta cttctgctgg
     3661 ttgtggtatt attctagctg ttttaggtat agtaactatg ccatggattg cttctgctat
     3721 gggagcagat ggtcaattat tgacagatag tattctttat ggatgtgtgg taattatcgc
     3781 tattcctgcc tatattttgc agtgcgaatt tcagtgttta tttgcaacag cagaaaagcc
     3841 aaaacttgga ttatatgttg caattatggc tggaattaca aatattgttt tagacgcatt
     3901 atttatcact gtttttaaat ggggattggt aggtgcagca ctggcaactg caatcggtca
     3961 atatgttggt ggtatcattc cattacttta ttttagccga caaaacaaca gtttgcttaa
     4021 atttaagaaa cctaaatttg attgtgatgt tttgaaaaag actgttgcaa atggttcttc
     4081 agaactaatg actaatgttt ccacatctgt tgtgagtatg ctgtataatg ctcaattact
     4141 aaaatatgct ggcgaagatg gcgttgcatc ctatggtgtt cttctttatg tagcgttact
     4201 atttcaagct gtttttattg ggtattctgt tggtactgcc cctataatca gttatcatta
     4261 tggagcaaaa aattatgacg aattaaaaaa tttaagaaaa agaagtttga ttgtaattat
     4321 ttgttttgct gtatttatgt ttataatagg cgaattttta tcaaaaccga ttgcttttat
     4381 ttttgtgggt tacgataata atttaatgga aatgacatta agagcattta tgatttactc
     4441 tttttccttt ttattttctg gttttggtgt ttttggttct agtttcttta ctgcattaaa
     4501 tgacggttta acttctgcgc ttattgcttt tatgcgtatt ttagtatttc aagttgtatc
     4561 agtacttata ttccctatat tctggggagt aaatgggata tggttttcta ttgttggtgc
     4621 tgaaatattg gcaactatta tgacggcttt ctttataata agaaaacaga aaaaatatca
     4681 ttattaaatc aatttggagg tatctatatg agcttaacaa tacaaaaagt aacttcatat
     4741 tttaaagatt tagaaaaatt tgaagaactt aataacgagg catttccaga tgaagaacgt
     4801 atgtctatac aagaaatgct taatttagtt caaagtaaaa aaatagaagt atctgcttta
     4861 tatgataagc aacaatttat tggtttttat gttttggtga ttgaaaatca tataggatat
     4921 atattatttc ttgcaatttc cccagaacaa cgctctaaag gatatggtgg aaaagcactg
     4981 tcacttatga aaaaacaata tcccaattgt caaattgttc ttgatatgga aactatagag
     5041 gacagtgctc ctaatttaca acaaagaatt ttaaggaagc aattttatct tcagaatggg
     5101 ttttatgaaa cagaatatct catggagtat aagggactag aaatggaagt tttatgtaat
     5161 cagaaaacct ttcaaaaaaa tgaatttgaa acattgttga aaaatcttaa aattagagaa
     5221 atttcatttc atttgtggaa aaagtaagaa agtgaggtat tagaaatgaa aagacgttta
     5281 gctggggaat gggaaccgat taaaggtgtc atgcttgcat ggccaccttt tcttccccat
     5341 aagtatattg tagatttaac gcaaaattat tctgtataca tgtgtgtacc aaataaagag
     5401 gaaattgaaa ttgcaaaata ttatttaagt aagtggggtg gaaatttaaa aaatttgatt
     5461 ttctgcattg ctccacaggg atttgataca ccatgggttc gtgattgggg tatgcaccct
     5521 atttttgatg aaaacggaaa actctttttg gctggtgcag aatataagat gtcaacacca
     5581 tttgtatcca taaataaacc agaaatcatg tttgattgcg aacataacct attaactact
     5641 aactcttatg aacaaaaaga agatcaatca caagagttga ttgcgaaaca gattggtttg
     5701 ccatttatga aattacctta tgcactaaca ggcggaaatc ttatgtcaga tggtttcaat
     5761 aaattgatgt ctatgcacat tttacagaca gaaaatcgtg cgaaaggaat ttctgacgaa
     5821 gaattttttt ctatggtggc aagagatact ggaatgaatg agtattatat attttctaat
     5881 tttgctgatt atggtttaca acatattgac tgttatttaa aaataattga tgaagaaaca
     5941 ttacttgtga gcagaacgcc aaaagaacac tatttgtatg aaagatatga aaaaatttta
     6001 gaagaagtaa aaaaggtaga aaccagatat gggcgtccat tcaaaatctt tcgtatagat
     6061 attgttccgt ttcaaaagga cagtgatgaa cttactgctt atgttaattc tgtgatttta
     6121 aatagtcgtg tctatgttcc aatgtatgga attgaacagg ataaaattgc aataaaacaa
     6181 tggcagaacg ctttgcctgg atatgaaatt aaaggatatg agtttgattt tgaaaaagag
     6241 ccagatatta taaaaaatcg tacaggatat gtgagaactg gctggggaaa tgaagatgtt
     6301 attcattgca ggacaagagc aatttgggat gagaatatgc tctatatttc cgtgaaaaga
     6361 ttagaagaaa tcgtttcaga gaatgatgct ttagaagtta ctattcaaat agttgattat
     6421 agtaaagctg gcttagatta tgaaaattgt agactgtttt atcgatacta tggttttcaa
     6481 acatgggaaa gtattaggtt agaagaaact acagaagcag aaatattttt tgccaatatg
     6541 ataggaaagt caggtgatat gattgaatat tttgtgcaag caaaaagttg ttctggaatg
     6601 tgtgaaacaa tgcctcctgt tgctccaaaa ggcacatata aagttactat aaagtaggag
     6661 gtgcatttta tttgaatgaa caagatcata catcatacca aaataagcag agatttttaa
     6721 taaatttctg ttatttggta gtgatattag gcttactaat gttattaggg aaatatgcgt
     6781 tttctgtatt acttcctttt gtttttgcat ttttaatagc aagtttattg aataaaccag
     6841 tcttatacat aagtgaaaaa ttactaataa agcgaggtat tgtagcaacc atatctgttt
     6901 ttttattctt tttaattgca ggaataatag taagtgtaat aggtacatat ctaatatatg
     6961 gaataaagga aatttttcat ttcctaccaa ccatgtttga ggaattggta atacctttga
     7021 ctgaaacagt tataaaagaa gtcgaactat tttctggttc attcaatttt tcgtttattg
     7081 aattaataga agcaaatatg cctgtattga tgaatgaaat gagtgttttc atagtttctc
     7141 tttcaaataa tatcgtatct ggtattactg atattgtttc gtctgttcca ttactattta
     7201 tgaaaacaat tattacaatc gttgcttcta tttttatagc aattgattat ccaacgataa
     7261 agagatttat agtgctacag atacctaaaa ataaaagtta tcttttagca gaagcaaaag
     7321 cgtttactat tgataccatt attaaatgtg gtttttctta tcttttaatt tttgctatca
     7381 ctttttctga attatatgtc ggttttgcta ttattaatat ttcttatgct ggaataattg
     7441 ctttattcat tgctctttta gatatactac ctgttttagg aactggaagt attctaatac
     7501 cttggggtat tatcagttta tttttagggg attattcaat ggcattagga attgcactac
     7561 tatatattgt gattactatt atcaggaata ttattgaacc aaaattagtt ggtaaacata
     7621 tggagttaca tcctgtattg gccttagcca gtatgctaac aggattacat ttctttggat
     7681 ttattgggtt gttcggtatt ctattgttaa ttgcgttttt gaaaaagtta aatgacaaag
     7741 aaatcataca tatattacat taggaaagaa tagttgtatg tatgtctctt ttatggagaa
     7801 aattgtgttt tttctaaaaa acgctggatg aaaaataatt ttgacataag gtatagcttt
     7861 tgaatgactt gattcaagag ggagaacttt tttaaaatat attacagcag gaaattcatg
     7921 gtgtcctatt actgtagata tttataactc aatttagata caaaatgctg gaaattaatt
     7981 atatagaaaa tatttccaaa aatttaaaaa ttatattgac aaagttaaaa aaataaacta
     8041 tcatataaaa caataagaaa ataaattgaa atcctgtgaa aaagagagta gcataaagct
     8101 agttgataga gagctgggaa tggtggaagc ctagtaacga attttattgt gaatagagct
     8161 ttggaggaga ttaccagaag taatagtatg gtaattggtg aatctcgcct taagagatta
     8221 gagtgggtaa gattatctta tcaattagag tggtaacgcg ggtgtatatt cgtctcttgg
     8281 tctttgacca agaggctttt ttaattttaa atgtacattt tgtagatact ttttaatttg
     8341 agttttaatg attattgtaa ttttaccaat aagagtggta ccgcggaaaa tcgcctctta
     8401 gtatttataa tgctaagggg ttttttgttt taattagagt attttaatct acgtattatc
     8461 aaatgattat catatagtaa agtggaaaga taggatttta agactatttg aatataaaaa
     8521 tggagggaac aaaatgaata aaataaaagt tggtattatt ggtgcaactg gatatgtagg
     8581 ggcagagttg attagattat taatgaatca tgataaagtc gaagtgagag cgataggttc
     8641 aaattcgtat gttggaaaag atatagttga tatatatcca agtataggat ataaaaataa
     8701 catgatatgt atagaaaatg aaaaagttat agatatgtgt gatgttgtgt ttacagccct
     8761 tccacatgga gttagtgaaa agtttgtaat aaaggcaatt aaatcgaaaa agaaagttat
     8821 agatttaggt gctgatttta gaataaaaga tgaagaagta tatagtaaat ggtatggagt
     8881 atcttttata gataaaatct tacataaaaa agctgtatat ggattaagtg aaatatacaa
     8941 agaagatata aaagatgcag atataattgc aaatcctgga tgttatccaa ctagtatttc
     9001 tttaccattg atgccattac taagttctaa attgataaaa aataataata taataattga
     9061 ttctaaatca ggtcttacag gtgctggtag agaattatcg attagtagtc actttacgga
     9121 ggttaatgaa aatatcactg catataaaat agggaaacac agacatactc cagaaataga
     9181 acaaaattta agtgaatcct gcaaagaaaa agtaagtgta gtttttacac caaacttaat
     9241 cccagtaaat aggggaatct tatcaactat atattgtaca aaagaagata atatttctat
     9301 aaatgatata cataggaaat taactgatta ttatgaattt aaagaattta tagaaattct
     9361 tccattagat aagatagctt ctttaaagaa tgttagattc tcaaataaat gtgtaatatc
     9421 attacatgaa aatggagata cattgataat ctgttcagct atagataata tgattaaggg
     9481 agcggcaggt caagctatac aaaacatgaa tataatattt ggtattgaag aaaatacagg
     9541 tttaaaaaat attgcaccat cattttagaa ataaaaaata ctaaactcat ataattagga
     9601 ggataaatat ggagattata aagggtggag ttacagtttc agaaggattt tttgcttcag
     9661 ggattcactg tggcttgaga aaaaataaag aaaaaagaga tttagctttg gtttattcag
     9721 atgttttatg tgatgcagca gcagtagtaa ctcaaaataa agtaaagggc aatcctgttt
     9781 atgttacaca ggaacacatt aaaaatggaa aagcacaagc tatcatagta aatagtgcta
     9841 atgctaatac ctttaatgga aaagaaggac ttataaatgc ttataaaatg gctaaattca
     9901 ccagtgataa attaaatatt aaagaaagtg atgttttggt tgcatctaca ggtgttatag
     9961 gaaagccatt aaatatagat ttaatagaag aaaatatgga tgaattagtg aataatctaa
    10021 gtaaacaagg tcatattgga gcaagagaag ctattatgac aactgatatt ataaaaaaag
    10081 aaatagcagt agcaataatg tatggagata aaaaaataac tattggaggt atggcaaaag
    10141 gttctggtat gatacatcct aatatggcaa caacgttagg atttataact acagatgcaa
    10201 atattgatgg agttttgcta aaagaagctt taaaaattgc tgttgacaaa agttttaata
    10261 gagttagtgt agatggagat acaagtacta atgatatggt aatgattcta gcaaatggaa
    10321 aagcaaaaaa tgatagaata aacaaaaaag atgaacatta tcaagtattt ttgagtgcac
    10381 ttacatatgt ctgtatagaa cttgctaaac ttgttgcaaa agatggagaa ggatgtacaa
    10441 aactaattga atgctatata aatggagcat taagtgaagc acatgcagta aaattagcaa
    10501 aaacagttat atcttcttca ttagtaaaga cagctgtatt tggagctgac gcaaattggg
    10561 gtagaatact gtgtgcttta ggttatgcag gtgaagaaat agatatggaa aaagttgata
    10621 ttatttttga gagtatgaaa ggatatattg aagtatgtaa aaatggaaat ggattagatt
    10681 ttaatgagga aaaagcaaaa aaaatccttg aagatgacga aataagtata ttagtagatt
    10741 taaatatggg caatgccaga ggaaatgcat ggggatgtga tttaagctat gattatgtaa
    10801 gaataaatgg aagttataga aattaggagg atatacttta atgattaata tagaaaaagc
    10861 aaatacactt atagaagctt taccatatat agaaaaacat cagggaaaga ctattgtagt
    10921 taagtatggt ggaagtgcaa tgaaaaaaga tggtctaaaa gaatctgtta tggaagacct
    10981 tgtgttaatg agttatgttg gaatcaatat agttttagta catggtggag gggcagaaat
    11041 caataagatg ctagcaaaag tggatataga aagtaaattt gtaaatggtc ttagatatac
    11101 agatgaagag accatggaaa ttgtaaaaat ggtacttgct ggaaaggtta ataaagacct
    11161 agtaaataaa atacatacaa aaggtggaaa agcagtaggg ctttgtggaa tagataataa
    11221 tatgatttta tgtgacccat ataaaaatta tgaacttgga tttgtaggag aaatcaaaaa
    11281 agtaaatgtt gagcttatag aatcttgtct aaagtcaggt tatatttcag taattgcaac
    11341 tataggtgtt ggagatgatg gagagactta taatataaat ggtgacacag cagcttctgc
    11401 tatagctaaa gagttaaatg cagataaatt gatacttctt acagatgtac ctggtttgtt
    11461 aagagagcca gatgaagaaa aatcattaat aacagaagtt atattagagg atgttgataa
    11521 attatttgaa gaaggtataa ttactggagg tatgatacct aaaatagagg gatgcgtaga
    11581 tgccttaaat aacggagtaa atagggtaca tattttggat ggaagagttc cacattctat
    11641 aataacagag ctatttactg atagtggaat aggtacatta ataagaaaag aaaatgaata
    11701 aaattataag tttaagaggg gagaataaga atgaataacg acaatacaat ttcaaaatgg
    11761 aatgaatatt ttatagatac atataaccaa cccaattttg tcatagatta tggtgaagga
    11821 agttgttttt ttgatactaa tggcaataaa tacatagatt ttacaagtgg atatggagta
    11881 tcttctcttg ggtatagtaa tagtaattta aagaatgctc taaaagagca agtagataaa
    11941 ttattacata cttcaaattt atattttaat gagccagtgt tgtattcagg agagaaaata
    12001 attaattcaa gtggaatggc taaagtatat ttttgtaatt ctggaacaga agcaaatgaa
    12061 acagcattca aaatagcaag aaagtatagt agtgacaaat atggtaatgg tagaggaact
    12121 atcataagtt taaaagactc ttttcatggt agaacaatga tgtctttaat ggcaactggt
    12181 atggacaaat accataagta tttttatcct cttcccgaag gttttaaata tatagaaaga
    12241 aataatattg aagatttaaa aaataatctt gattccacag tttgtgcaat aattcttgaa
    12301 gcgattcaag gcgaaggtgg agttaatgtt cttgaaaaag attatgtttt gcaaatagta
    12361 aaaatatgtc aagaaaagga tattgtagtg atttttgatg aagtccaatg cggtatagga
    12421 agaactggta aactttttgg ctatgaatat tttgatgtaa aaccagatat agttaccgta
    12481 gcaaaagggt tgggagcagg aataccagta ggaggagtat tggtaaataa aaaactttca
    12541 aaagtactag gaaaaggaga ccaaggtaca acttttggag gtaatttatt agctatggtg
    12601 gcagcttcag ttgtattaga tgagatatct aaagatggtt tttataatga agtattagaa
    12661 aaaggaaatt atataagaaa atctattgaa agttttaata acaaagttgt attaaagaca
    12721 aaaggtattg gtctaatgat aggaattgaa actaatattg aatctagtat tatagaagag
    12781 aaagctagaa aaaaaggatt gcttattttg actgctggaa aaaatgtatt gagattttta
    12841 ccaccattaa caatcagtta taaggaaata gatgaggctc ttgaaatcct aaaagatata
    12901 ttactagaaa ttaactaaat attcaagaaa tagttgatta aagataaaaa ttagaagtaa
    12961 ataaaaaagt aaagtgctaa caagtttaat tgaatttaaa aagtttataa gaaggggcga
    13021 attaatatga ataatataag tttaaaaggt aagagttttt tgacattaaa ggattttaca
    13081 aaagaagaga ttagatattt attaacttta tcaagttatt taaaaactaa gaaaaaagtt
    13141 ggtataaaag gcgatttgct acaaggaaaa aatatagctt tattatttga gaaaacatca
    13201 acaagaacta gatgttcatt tgaagtagca gctcatgatg aaggagcaca tgtaacttat
    13261 ctaggtccaa atgattgtca tatgggtaaa aaagagtctg tatcagatac agcaaaagtt
    13321 ctaggaagat tctatgatgg aatagaattt agaggcttta aacaagaaac agttgaaagt
    13381 ctagcaaaat attctggtgt acctgtttgg aatggactta cagatgaata tcatccaaca
    13441 caaatactgg cagatttttt aacagtaatt gaaaatgtag ataaagattt gaataaagtt
    13501 aaatttgtat atgttggtga tgctagaaat aatatgggaa actcactgat gataggatgt
    13561 gctaaaatgg gaatacattt cgtagcacta gcacctaagg aactttggcc aaatgaagaa
    13621 ttggttaagg aaatgaagga aatagccaag aaatccaaag gagatataat gttgactgaa
    13681 aatattgatg atgttaaagg tgcagatgtt atatatacag atgtatgggt ttctatggga
    13741 gaagaggagc aatatgaagc aagaataaat caacttaaag attatcaagt aaatatggat
    13801 atgataaaga aaactgaaaa tgaaaatgtt atatttttac actgtcttcc agcatttcat
    13861 gatttagaaa ctaaggttgc tcaggagatt tatgaaaagt tcggattatc agaattagag
    13921 gttacaaacg aggtatttaa ttcaaaatat tcaaaagtat ttgaagaagc tgaaaataga
    13981 atgcatacaa taaaagctgt tatggtttct actatgactg atgtaaatat gaacaataat
    14041 ttaatataga tatgttttaa tatagacttt actttaatga aaatttataa aatattttaa
    14101 atatattatt caaaaactct cattatatgt taaaataact tatgtatcaa caaattaaga
    14161 gggaggattt gagatatgaa aaaaatcata ttagctttaa caactttttt attagcaata
    14221 tctttaacag catgttcaag tgcaaaacca gaagatacaa ttgatagttt ttttaacagt
    14281 gcgaaaaagt ttgactttga aggcatgaat aaggtaatgg aaaataatga cgaaaaatat
    14341 aaggatatat taaaggaatt agatacaaaa gacccaaatg cccaatatgt tttagattat
    14401 ctaaaacaga atgcatctaa aatcacatat acaataaaag acagcgaggt taaaggtgat
    14461 aaggctacaa tcaaagtaga atgtaaattt atagattcaa caccattact tcaagaaata
    14521 gtagctgagg catttacaaa gatgattggt atgagttttt caggacaaga cttaactgat
    14581 gagaaaacaa ctgaaatgtt agtatcaatt atgaaagaaa aacaaaaaag cgttaaagag
    14641 acttatgtta caaaaaatgt agagtttgaa tgttctaaaa aggataataa atggataata
    14701 agttcagtaa atgatgcagt agctgatgta ttgttatcta atcttgttac agcaggacaa
    14761 gaattttcta actctatgaa tacacttgaa taatattgtt taggtgtagt aaagtaataa
    14821 atatatgatt atttttataa ataaacatat aaatatttat gaaggatacc atttttggta
    14881 tcctttttat taatataatg aaatacatgg ttaatttcta tattgtcaag aaactatgat
    14941 atatgttata agtctat
//
LOCUS       DAKBNN010000091        14618 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000091 DAKBNN010000000
VERSION     DAKBNN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.091"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..569
                     /locus_tag="OUB02_002274"
     CDS             <1..569
                     /locus_tag="OUB02_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886031.1"
                     /translation="KIWEDFFETKNQNIYKALLEISNYFKSKGLKIGIDTFSPFISYF
                     TGQDLEKLQEVADFIKPMMYRITKAPAGLPFEFERFIDESIHKNKEIGKRNFLDILGI
                     KEYEGNVYPIEFVLEEIDFMTRNLNKDIYVGVEINKKEIIAPVTPKYISENISNINKT
                     TVKGYVLSWDLLSAPKENIDEIIKWFRN"
     gene            582..2078
                     /locus_tag="OUB02_002275"
     CDS             582..2078
                     /locus_tag="OUB02_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCT8886032.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSTSIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            2101..3051
                     /locus_tag="OUB02_002276"
     CDS             2101..3051
                     /locus_tag="OUB02_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886033.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            3072..4034
                     /locus_tag="OUB02_002277"
     CDS             3072..4034
                     /locus_tag="OUB02_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCT8886034.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIAKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            4406..5038
                     /locus_tag="OUB02_002278"
     CDS             4406..5038
                     /locus_tag="OUB02_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT8886035.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            5449..5541
                     /gene="kdpF"
                     /locus_tag="OUB02_002279"
     CDS             5449..5541
                     /gene="kdpF"
                     /locus_tag="OUB02_002279"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCT8886036.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            5582..7264
                     /gene="kdpA"
                     /locus_tag="OUB02_002280"
     CDS             5582..7264
                     /gene="kdpA"
                     /locus_tag="OUB02_002280"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCT8886037.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            7289..9346
                     /gene="kdpB"
                     /locus_tag="OUB02_002281"
     CDS             7289..9346
                     /gene="kdpB"
                     /locus_tag="OUB02_002281"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCT8886038.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            9365..9991
                     /locus_tag="OUB02_002282"
     CDS             9365..9991
                     /locus_tag="OUB02_002282"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCT8886039.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            10228..11136
                     /gene="cysK"
                     /locus_tag="OUB02_002283"
     CDS             10228..11136
                     /gene="cysK"
                     /locus_tag="OUB02_002283"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCT8886040.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            11163..11753
                     /gene="cysE"
                     /locus_tag="OUB02_002284"
     CDS             11163..11753
                     /gene="cysE"
                     /locus_tag="OUB02_002284"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCT8886041.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            11913..12101
                     /locus_tag="OUB02_002285"
     CDS             11913..12101
                     /locus_tag="OUB02_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCT8886042.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(12155..13024)
                     /locus_tag="OUB02_002286"
     CDS             complement(12155..13024)
                     /locus_tag="OUB02_002286"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HCT8886043.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(13221..14465)
                     /locus_tag="OUB02_002287"
     CDS             complement(13221..14465)
                     /locus_tag="OUB02_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886044.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLGEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         5414 a         1661 c         2644 g         4899 t
ORIGIN      
        1 ataaaatatg ggaagatttt tttgaaacaa aaaatcaaaa tatatacaag gcattattag
       61 agataagtaa ttattttaaa agtaagggat tgaaaatagg tatagatact ttttctcctt
      121 ttatttctta ctttacaggt caagatttag aaaaattaca agaagtagca gattttataa
      181 aaccaatgat gtatagaatt acaaaagctc cagctggttt accatttgaa tttgaaaggt
      241 ttattgatga atccatacat aagaataaag agattggaaa aagaaatttc ttagatattt
      301 taggaattaa ggagtatgaa ggaaatgtat accctataga atttgtacta gaggaaatcg
      361 attttatgac aagaaatctt aataaagata tatatgtagg agttgaaata aataaaaagg
      421 aaattatagc tcctgttaca cctaagtaca taagtgaaaa tatttcaaat ataaataaga
      481 caacagtaaa aggatatgtt ttatcttggg atttgttatc agcaccaaaa gaaaatattg
      541 atgaaattat aaaatggttt cggaattagt gaggtgatat agtgagtaag atattggaaa
      601 tgcaacaaat atataaaagt tttggaaagg tagatgtttt aaaagggata aattttgagt
      661 tagaaaaggg agaagtaaga gctcttttgg gagctaatgg agcaggaaaa tctacactta
      721 ttaaaatttt aggaggagtt cattcccaaa ctaaaggtaa agtaattttg actggtaatg
      781 aaataatgtt taaaaatcca catcattcta aagaaaatgg tattagtata atttatcaag
      841 aattaagctt gataccaacg cttacagtac ttgaaaatat ttttcttggt agagaaattt
      901 taaaaggaag tgtgttttta aacaagaaag acatgcttga ggaatatatg aaaatttgtg
      961 aggattttga ttttaatata gacccacata tagtagtttc taatttaagt gtagcaaacc
     1021 aacaaatggt tgagataatg aaagctgttt catgtaattc tgaaattatc ataatggatg
     1081 aacctacaac atcacttact aataatgaaa aagatgggct atttaaaata ataagtagac
     1141 taaagtctaa aggtaaaaca atagtctata tttctcacat attagaggaa gtttttaaag
     1201 tatgtgatag tacaagtata atgaaaaatg gaattatgat tggaacttat gatgtagagg
     1261 atttaactaa attaaagata actcaactta tgacaggaaa tgataatcac aaacttattg
     1321 agaagaagga ttatacattt gcaaaatatg aagagccacc agtactagaa gtaaagaata
     1381 taagctggtc taacaaaata aaaaatgttt catttaaagt ttatagaggc gaagttgtag
     1441 gtcttgcagg tcttgtaggt tcaagaagaa ctgaaattat aaatttgata tttggtatag
     1501 ataataaaga tagtggagac atttttatca atggtaaaaa agtagacata acttctccta
     1561 aggttgctat aaaaaacaaa ataggattga ttcctgaaga tagaaaaaat ctaggattaa
     1621 ttttagaaca agctatatac acaaactcat catcagtaca aattgataaa ttcaagaaat
     1681 tcttgtttat agatagaaat aaagaaattg agttttctaa aaaaggtgtt aaggaattag
     1741 gaataaaagt tagtgatgta aagcaagaag taaaagagct tagtggtgga aatcaacaaa
     1801 aagtagtagt ttctaagtgg ttagagaaag atttagattt aataatatat gatgagccaa
     1861 caaagggaat agatattgct gcaaaagaag atattttcaa tacaatcaaa gaattttcaa
     1921 gtaaaggaat aggagtaata tttatatctt ctgatttaga agaagtaata agagtaagtg
     1981 atagaatttt agtaactaga gaaggttcta ttgtagatga tttaaaaaat gagaatatac
     2041 aggttcaaga tataatgaac agtatattta atgtataaaa aatgaaaatg gaggactgag
     2101 atgaagaaag cttttgagaa gttaaattta agagattatg gagtattatt aggtttttta
     2161 gctttatgta tagctataag tattgcaact ccatcatttt taggtaaaca aaatatactt
     2221 aatcttttac gacaatcttc tataattgga ataatttcag caggtatgac ttttgttata
     2281 atatctggaa actttgatat atcagttgga gcagtagcag ctcttgcagg tgctataaca
     2341 atgaaatttg cgactggtgg aactaatttg tttgcatcca tgttcttagg tgtagcgatt
     2401 tgtgcagtaa taggattaat caatggtata gtagttgcaa agataaatgt accatcatta
     2461 atagccacta tggctatggt cactattgta agaggattgc tgttgatgct tacaggagga
     2521 tatccaatta ctgaaaatat accaatactt gattacattg gaaatggata tttgtttaat
     2581 ataccaatac cagtagtaat tttctttata gtggtgttag tttcgtttat tgtacttaat
     2641 aaaactaagt ttggtagata tgtttattct gtaggaggga atcaagaagc aagtaaactt
     2701 aatggtataa atgtagattc tcacaaagtg aaagtgttta ttattaatgc tgtacttgca
     2761 ggaatagctg gaatcgtact aacaggaaga cttgggactg caactgcgat tgcaggtgaa
     2821 ggatatgata tggatgcaat tgcatctgta gtaataggag gtacatcagt ggcaggaggt
     2881 tctggttctg tacttaaaac tgttatagga gtattactta tgagtgttat aaacaacagt
     2941 tttaatcttc ttggaataga tgtatatttc cagtacatat ttaaaggatt aataatttta
     3001 gcagcagttg gatttgattc ttatagcaag aaaaaacttg cttcaagata aaaaataagg
     3061 gggatttttt tatgaaaaaa ttaatgaaac atttagcatt gttgctatca cttgttatga
     3121 tatttggttt agttggttgt tcaaatggtg gtgacaaaga aaaaaatgga gaaaagaaaa
     3181 tagcagtatt actaccgggt tcaacaggtt actttgtagc aactaaacaa ggtatagatg
     3241 caaaatctaa agaattaggt gtaagcgtgg aatatgcaga tgctcaatgg gatgctagta
     3301 aacaattatc acaagctgaa gattttatgg ctaaaggggt agacatgatt gcattatgtg
     3361 gagttgattc tgctgtaagt gagagaatag ctaaagctgc taatgattca gacgttccta
     3421 tagtagcatt tacaaattct ataggaagta acccaactgg agaatttaaa ggtttagtta
     3481 catatatagg acaaaatgag gaagaaacag gagcattaac tggaaaaatt gctaaaaact
     3541 tattaggaga aactggtggg aaagctgtat taatagaagg agtaccagga actactccac
     3601 aagtattaag aaaaaaaggt cttgaaaaag aattaaaaga tagtaatata gaaatagttt
     3661 ataatcaaac ttcaaattgg gagaaagagc aagctttaaa agtaacagaa gatttaattc
     3721 aaaagaaaac tgattttaat gttataatat gtcaagatga taattctgct acaggtgcag
     3781 gtcaagcttt aaaagatgct ggtcttaagg ataaagttaa agtcataggt ttgggtggaa
     3841 gtaaagatgg attaaaagct ataactgatg ggttaataga tggtacaact tatatgtctg
     3901 ctgtagaaga aggtggatta gtcatagaaa aggcatctaa attccttaaa ggtgaaaaaa
     3961 tagaacctgt tacacaaatt aaacaagttg aagttaacaa agataatatt tcagagttca
     4021 agggtgaatg gtaaattgtc atagataata aataagattt ttagagttac tttagaaact
     4081 atataaagta actctttttt taatttaatt tagtgataaa ttattgaata gtatttctaa
     4141 acaaataaaa aattaatata aacaaagact taattatact aaattataag taaaactaat
     4201 ataaacttat ggaaaaaagg aaaacgtcta aagtattgga attactatag tgtaggtgtg
     4261 gtattaaaaa aacataaaaa aacataaaaa aattgttgaa aaaaatatag aaattttgta
     4321 taattaaaga tttgaaaagt gcggtataca aagccaattc ttttgtatat aatatatata
     4381 aattcagaaa aaggaaggga tatgtatggc caggcggtta tttttagaaa ggattggact
     4441 tatattttta ggttcagcaa tattagcatt tggagtttat aatttttatt atttaaacaa
     4501 cataacagaa ggtggagtgt taggtatttt actactgtta aaaaatttat tcaatataca
     4561 accagcaata gcaaatgttg taatagatgg tttactactt ttagtaggat ataaattttt
     4621 cggtaaaaaa ttccttatat attcaatagt agcttctata actttttcag tgttatatga
     4681 cttatttgaa gctataggac ctctagtacc acaatcacaa aatatgcttt tatcaactat
     4741 tttggcaggt gtaactgttg gaactggtgt aggtatagta gtaaaagcag gttgtgcatc
     4801 aggaggagac gatgctttag cattagtaat ttctaagact acatctttga acatagggca
     4861 agtttattta gcaacagatg ttattgtatt gttgttatca ttattttatc tatcagcgtt
     4921 tgatatcttt tattctttga ttgctgttac aattagtggc aaggtaattg attttattta
     4981 ttatcatggt aaaagtttgg atatggatat aagtaatgat atagttccag aatgttaata
     5041 taaattaaat aactttagat tttattaggg gtggcagatt tgccaccttt tttgtgtcta
     5101 aatctttata gaaagtataa tcccatctag gtttttgtaa atctcaaata tagaattatg
     5161 ttggaatgac tgggaatacg attgctagat gtgcatttta aaaaccaaat tttatattta
     5221 tgaaatacta atcattaatg gatgtttctt aatcctatct ttatacctaa aatgataaga
     5281 taaatgaagt agcaatacaa aactaaaatt tgtaaaagag attaaattaa atggaggagc
     5341 taaaatgttg gatatagtta tgatagcatt gttattcata ggatttgttt ccatgaagtt
     5401 acttgctgat tggtgtggga aacaaactga aaaaaaataa tgtaatgtat ggagggatta
     5461 cctatgtggt ttctggcagt tgtaattata atgttaataa tatatttggt atatgcgctt
     5521 attaatcctg aaaaatttta aacagtataa ggctaataag taagttgaat tggggggaac
     5581 aatttttatg gtgcagatag taatagtgtt agcgattttt atgattttag taatacctat
     5641 gggcaagtat ttatatcata tagctactaa tcaaaaaaca tttggagata gactatttga
     5701 taaagtggac aattttatat ataaggtatg tagtatagat aagaaaaaag aaatgaactg
     5761 gaaacagtat gcattagctt tattatttac aaatgcagtt atggtattta taggatatat
     5821 catacttaga actcaaagta tgcatatttt taatccaagt ggaattaaaa gtatggaaca
     5881 aggactatca tttaatacaa taataagttt tatgacaaat acaaatcttc aacattattc
     5941 aggtgaatca ggactttcat actttagtca gatgacagtg ataatatata tgatgtttac
     6001 atcagctgca acaggatatg cggctgctat ggcatttgta agaggtctag tcggtaaaaa
     6061 gaaaacattg ggaaattttt atgtagattt aattaggata acaacaagag tacttttacc
     6121 aggagcttta attattgggc ttatattagt tacacaagga gtaccacaaa cttttgctgg
     6181 gacagaaaca gtgactacta tagaaggaaa gcttcaagat atagctagag gtcctgtggc
     6241 agctcttgaa tcaataaaac atttaggaac aaatggtggt ggattctttg gttcaaattc
     6301 atcacaccca tttgaaaatc caacaataat atcaaatata gtagaaatat tatctatgat
     6361 gatattacca ggagcttgtg ttgttgcatt tggtcacatg ataaagaata aaaaacaagg
     6421 ttgggttgtg tttggcgcaa tgtcaatcat atttttaata ggtctagtag tgtgctttaa
     6481 agcagaaagt gcaggaaacc ctattttatc acaattaggg cttaatcaaa gtatgggaag
     6541 tatggaaggt aaggaagtta gatttggtat agcacaatca tcattattca caacagtgac
     6601 tacttccttt acgacaggta cagttaataa tatgcatgat acactgactc ctttaggagg
     6661 attagtacca cttttaaata tgatgctaaa tgtagtattt ggtggtaaag gcgtaggtct
     6721 gatgaatatg ttgatgtacg ctataatagc agtattctta tgtggattaa tggtaggaag
     6781 aacaccagag tttttaacta agaaaatcga aggaaaagaa atgaagctta ttgctttact
     6841 tattatatta catccacttt taatactaat gttctctggt ctttcagtag caataccagc
     6901 aggacttgaa ggaatttcaa atcctggatt ccatggatta tcacaggtat tgtatgagtt
     6961 tgcatcttca gcagctaata atggttcagg atttgaaggt ttaggagata acactatgtt
     7021 ttggaatata acaacaggta tagttatgtt ctttggaaga tatgtgtcta ttatagtact
     7081 actagcaata tcaagtttgt tagcatctaa aaaagctgta aatgaaagta taggaacact
     7141 tagaacggat aattttacat ttacaatagt tctagtactt gttgttttaa ttgttggtgc
     7201 tttaacattt ttcccagcct tagcgcttgg acctatttcg gagcatctag tgttgtggca
     7261 ctaaaaaatt gggagggagt aaagttttat ggaaactaaa aaaacaaagt ttattacaaa
     7321 agatattttc aaaacttcca ttatagaagc ttttaaaaaa ttaaatccta aatatatgat
     7381 gaaaaatcca gttatgtttg ttgtagaaat tggatttata gtaacattga tacttacaat
     7441 agtacctagt ttgtttggtg atgaaggcaa taacttaaga gcttataatg ggattgtaac
     7501 aataatctta tttataacag tcctttttgc aaattttgct gaagctgtag ctgagggaag
     7561 aggtaaagct caagcagata cacttaagaa aactagaaaa gatacagtag caagggttat
     7621 agatacgtta ggaaatgaaa aaatgataag tgcaagtgag ctaaaacttg gtgatattgt
     7681 tttagtaaat gctggagaag ttattccaaa tgatggagaa gttatagagg gcatagcttc
     7741 tgtagatgag tcagcaataa ctggagaatc tgcacctgtc atgagagaat caggtggaga
     7801 tttcgcatct gttacaggtg gtactacagt tgtaagtgac tggttaaaga taagaataac
     7861 aacaaaacca ggagaatcgt tcttagataa aatgatagct cttgtagaag gtgcttcaag
     7921 acaaaaaacg ccaaatgaga ttgctttaaa tactttgtta gtagggttaa cacttatatt
     7981 tatggttgtt ttaatatctc tttatccaat ggcaaactat gcaggtgtaa agattccaat
     8041 ttctactatg atagctcttt tggtatgttt aataccaact actataggtg gattgttgtc
     8101 agctattggt attgcaggta tggatagagt aactagattt aatgtaattg caatgtcagg
     8161 aaaagcagta gaagcttgtg gagacgttga tactatgatt ttggataaga caggtacgat
     8221 tacttatggg aatagacttg catcagaatt tatagcagtt ggaaatgcag attcaaaaga
     8281 tttaataaat tattctgtta tgtgttcatt aaaagatgat acaccagaag gaaaatctat
     8341 agttgattta ggtatgaaat taggttcaac aggtaagaca aaagaggcag aggaagctga
     8401 gtttgtagaa ttttctgcac aaactagaat gagtggtatt aacttagctg atggtagagc
     8461 tataagaaaa ggtgcttatg atagcataat aaaaagagta aaagaatctg gtggtacagt
     8521 gccagaagat ttagaagaaa atgtaaatcg agtagctaaa ctaggaggaa cacctttagt
     8581 agtgtgtgta gataatgaga tatatggaat tatctactta aaagatacag taaaatcagg
     8641 gcttgtagaa agatttgcta gacttcgaga gataggaata aagacaataa tgtgtacagg
     8701 ggataatccc ttgacagcag ctacaattgc aagagaagct ggagtagatg gatttattgc
     8761 agaatgtaaa ccagaagata aaatagatgc aataaaaaaa gaacaattag agggtaaaat
     8821 agttgcaatg acaggtgatg gaacaaatga tgctcctgca cttgctcagg cagatgtagg
     8881 tcttgcaatg aatagtggta caacatctgc aaaagaagca gcaaatatgg tagatttaga
     8941 ttcagaccca actaagatat tggaagttgt tgaaatagga aaacagcttt taataactcg
     9001 tggggctttg acaacattta gtatagcaaa tgatgttgca aaatattttg cgattatacc
     9061 agctatattt actcttgcaa tacctcaaat ggagatatta aatgtaatga aactttctac
     9121 gtcatttagt gcgatattat cagctttaat atttaatgct ataattatcc catgtttaat
     9181 acctcttgca atgaagggag taaaatatag acctatgaaa tcacaagcta tgttaatgag
     9241 aaatatgctt atatttggtc ttggtggagt tatagtacca tttttaggaa taaaattaat
     9301 tgatttattg attacacctt tagtaatggt tcttaattta ggttaataaa tggaggatat
     9361 aaaaatgaaa acattaaagc cagcattact tgtaagtatt gtattgttag ttgtttgtgg
     9421 acttgtatat ccacttgttt tgacaggtgt aagtcaagtt gcatttaaag ataaagccaa
     9481 tggaagtatg atagaagtaa atggtgtaaa agtaggttca gaacttattg gtcaaagctt
     9541 tacagatgcg agattcttta agggaagagt atcatcagta aactataata cttatacaaa
     9601 agaagattta gtaccagata aagatggaaa tacatcttat ggaggtgtat catcagggtc
     9661 ttttaattat ggagctacaa atccagaact tcatgataga gtacaaaaag atatagaaaa
     9721 gtttttaaag gataatccaa ctgtcaaaag agaggatata ccaacagatt tacttacagc
     9781 atcaggttca ggacttgacc ctaatataag tccagcctct gcaaaaattc agataccagc
     9841 tatagcaaaa gcgtctggaa tatcagaaag taaattacaa aaaattgtag atgataatac
     9901 ttccaaaaaa ctttttggag ttttaggtga agatagagtt aatgtattaa aagttaatgt
     9961 agaggtcgca aaaatattag gtcttatata gtattttgtg gaattttagc aacaaaatat
    10021 tactcaagat ttaatttaaa ggttaaattt taaagagtga aaatcgataa tgattttcac
    10081 ttttttattt gaatattact aatatttgga ataaagtgat tgtaagtata aaatccaaaa
    10141 aaaggttgca ttatcaaaat gataatacta taatatattt atcgttatca atttgataat
    10201 agatttggac atataggagg aaatagtatg ttatataata acgcattaga gttgatagga
    10261 aacacacctg tagtaaggct gaataattta ggctgtacta atgggtgttc taacatatat
    10321 gtaaaattag aaaaagtaaa tccagcagga agtataaaag atagagctgt ttatgaaatg
    10381 ttagagggct tagagcaaag aggagaatta aaaaagggag atgtactagt tgaagcaact
    10441 agtggaaata ctggtattgc attatctatg ataggaaaat taaaaggata tgaagtagta
    10501 atcgtaatgc ccgaaacaat gagcgttgag agaagagatt taatgaaggc ttatggagca
    10561 aatttggtgc ttacagatgg taaagctgga atggcaggtt ccatagaaaa agcaaatgaa
    10621 cttttaaaag aaaatccaaa ctataaaagc ttaaaacaat ttgaaaataa agataatcca
    10681 aatgcacatt ataaaacaac agcagttgaa attatgaatg atgtcaaaga tttagatata
    10741 ttcgtatgtg gagttggaac aggtggtact ttaataggaa ctgcaagata tttaaaagaa
    10801 caaaatccaa atataagagt aattgcactt gaaccaaaaa aatctccagc tatatcagaa
    10861 aacaagactg gacctcataa aatacaagga ataggtgcta actttgttcc tgaaaattat
    10921 gattctagtg tagttgatga agtaatatta gtagatgatg aagatgcatt tgatacagtt
    10981 aaattacttg cagcaaaaga gggaatttta gtagggatat cttctggagc aaatatattt
    11041 ggtgctatag agatggcaaa aaagtatcca gataaaaaga tagttacagt agctccagat
    11101 ggagttgata agtatatgtc tatgggaata ttttaataat tttatttgag ggagtgaata
    11161 ctttgtttaa gaaaataaat aaagatattg aatatataat gaagaatgat ccagcagcta
    11221 ggtctaagat agaagttttt ttactttatc catcagttca tgcaatgata atgcatagga
    11281 tggctcatgc tctttataag aagaagaagc tttttacagc aagattaata tcccagatat
    11341 ccagatttat gactggcata gaaatacatc ctggtgctaa aatgggggaa ggtatattga
    11401 tagaccatgg aatgggtgtt gtaattggag agacagcgga agtaggtaat agggtgacaa
    11461 tatatcaagg agctacttta ggagctacag gaaaagatac tggaaagaga catccaactg
    11521 ttggtgatga tgtattaatt ggtgctggaa caaaaatact aggaccttta aatataggtt
    11581 caaattcaaa aattggtgca aattctgttg ttgttaagga tgtacctaat ggtgcaacag
    11641 ttgttggtat accagcaaaa atagtaaaaa taagaaacct agaacctgta aaaaagaata
    11701 aaaaagaagt gtcctatgag tatgatgaat tagataatgt atattatata taggatttat
    11761 ataactataa aataaacttt ataaaaatta ttttaaaagg tatttttttc aaatttgagg
    11821 acataataaa aaagaactga atgttacagt tttggttcat caatctttga taaataagat
    11881 tttaaatttg tagcgtctag gaggatttta ttatgaaagc aaatgttaat caagatactt
    11941 gtataggttg tggattatgt ccatcaatat gtccagaagt atttgatatg aaggatgatg
    12001 ggaaatcaca tgttatagtt gatgaagtac caagtgatgc tgaagaatca gcagcagaag
    12061 ctagagaaag ctgtccagtt gatgcaatag atgtgcatta ataaaataaa tgcctataaa
    12121 aattgaattt tcaattttta taggcatttg tcttttattc agttagtatt tcaacacctg
    12181 tttctgtaac taaaaccata cattcccatt gagcagataa tttaccatct tttgtataaa
    12241 tagtccaatt attttcttca tcaacaaaaa tttcgtgagt tccttcattt accataggtt
    12301 ctattgtgaa tatcatccca ggaaccaata gcatctcagt ccctttttta gatacataac
    12361 ttacaaaagg gtcttcatga aagcttaatc caactccatg accacctatt tctctaacaa
    12421 cagagtatcc atttttcttt gcataagagt tgatagcatc acctatatct cctaaaaatc
    12481 cccatggttt ggcaacttca agaccttttt ggacacattc ctttgtaact tgaactaatt
    12541 tttttgcatt ttcattaaca ttacctatca taaacattct tgaagcatct gaatagtatc
    12601 cattaaaaat agtagaaaca tctacattta taatatctcc atcttttaag ataatgtcct
    12661 tacttggaat accatgacaa acttcattat ttactgatgt acatacactt ttaggaaatc
    12721 cattatagtc tagtggagca gggatagctc cattttgtag tgtaaagtca tgaaccaact
    12781 tatttatatc ttctgtagac attccaattt cgattttatc tgaaatataa tccaaaattt
    12841 ttgtgtttat tacagcactt ttttttattt cctcaatttg actttcattt tttataatat
    12901 catgagttgg tacaatatga cctaacttag caaatgaatc tattttatta tccatatcca
    12961 tatgacattt tttatatttt ttagtacttc cacaccaaca gctatcattt ctatttaact
    13021 tcataacttt tcatccttct tttattattt atttaaatta tgggttacat aaactatttt
    13081 aaaacataac cattataaat attataacat aaatactaag aaatccttta tagatagtga
    13141 tttattcaaa gtataaatta ttatatggtt attgttgatg aaaagaggat agttaaatgt
    13201 attattggat attagcgcac ttaatagttt agttcaagtt tttcaagaaa atcatatgtt
    13261 agaggacaat tttcctttaa agtatttttt gaatcttcat caagataata atgtacaaat
    13321 gattctgcaa aaaattcttc tataaacttg cttatatatg catgtgcaac ttgcttaggg
    13381 tcataaagct tatatctttc ctgagcaaat acttccataa attcttcaga atttgatttt
    13441 ttatttaaaa catttttatc tatagcatgt gcagtttcat gtaattctaa attttttgat
    13501 gaatgtacat ttgcatagct aggattacta taacctattt tagccactat agaattattt
    13561 ctatatatac caggtacatc tttccaagtc ttgcctgatc ttacccaaca tggaggtaat
    13621 tgatatttta aatcactaaa ttctggttca tcagttagat ttgaatttat taattttatt
    13681 tttatatcct tttctttaag tgtctgtaaa tatttaatat ttatttttgc aagtctgctt
    13741 ttcatatttt caacttcatc tttatcataa ttcaagtcat caggaagtat aagcatttgt
    13801 tcaattatat ctatactact gtcttttata ttttcagatt cttcatcaaa tttatattga
    13861 agagtattat tttcatagat agttatattt gtattatttt ctatagaata agagtgaata
    13921 tcctctaaat ctaaaatatt catttgttgt aaaatatgtt ttgtttcgcc actccatact
    13981 actaaagaaa gtgaattaaa attaattagt ggattttctt ttgcttttat atcattgctt
    14041 atatctacat caaatacata ttcaattgag tcttctatgt taagactatc tgtattcttt
    14101 ttaatatata aatacgcttg ttttaagtct atataaatct cacctaaaga ttcgtcataa
    14161 aattctgata aattattagt aaagagttct tcacttaaaa gttcattttt atcagttata
    14221 ctttcaatta aaatattaat ttcttttatc atatcgtaaa tacttatttt tctatttgct
    14281 ttttcactag atacattgca tggcgcaaga gcaatgcaat aaaaatcttt ttctttttta
    14341 taaaaggctt taagtgattt tgataaaagt gtaaagtcta cacctatctg taagttgacc
    14401 ttaagtgagt cattttgtaa taattcagta ttatttcttt tagctgtatt taaattgttt
    14461 tccataatca ttgccccctt tttattaggg tatcataatt tttttatatg tgtcaatttg
    14521 tttttttgaa aattataatt tttttgaaaa ttttattttt tataatttgt gaatttttta
    14581 ttatagaaat aaatatgtta ttattatgtc aaaattta
//
LOCUS       DAKBNN010000092        14601 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000092 DAKBNN010000000
VERSION     DAKBNN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.092"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(26..898)
                     /locus_tag="OUB02_002288"
     CDS             complement(26..898)
                     /locus_tag="OUB02_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8886045.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            1314..2717
                     /locus_tag="OUB02_002289"
     CDS             1314..2717
                     /locus_tag="OUB02_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886046.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            2744..4288
                     /locus_tag="OUB02_002290"
     CDS             2744..4288
                     /locus_tag="OUB02_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HCT8886047.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            4319..5710
                     /locus_tag="OUB02_002291"
     CDS             4319..5710
                     /locus_tag="OUB02_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCT8886048.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            5903..7399
                     /gene="abfD"
                     /locus_tag="OUB02_002292"
     CDS             5903..7399
                     /gene="abfD"
                     /locus_tag="OUB02_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HCT8886049.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            7534..7809
                     /locus_tag="OUB02_002293"
     CDS             7534..7809
                     /locus_tag="OUB02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886050.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            7833..9140
                     /locus_tag="OUB02_002294"
     CDS             7833..9140
                     /locus_tag="OUB02_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HCT8886051.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            9160..10278
                     /locus_tag="OUB02_002295"
     CDS             9160..10278
                     /locus_tag="OUB02_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HCT8886052.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            10565..11485
                     /locus_tag="OUB02_002296"
     CDS             10565..11485
                     /locus_tag="OUB02_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCT8886053.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGNKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            11600..12757
                     /locus_tag="OUB02_002297"
     CDS             11600..12757
                     /locus_tag="OUB02_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCT8886054.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(12842..14341)
                     /locus_tag="OUB02_002298"
     CDS             complement(12842..14341)
                     /locus_tag="OUB02_002298"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCT8886055.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5606 a         1751 c         2670 g         4574 t
ORIGIN      
        1 aatattatat ctgtataata taaatttagt ttatatgaat agatgccata tactcaataa
       61 aaagtttcat actatgagat atgtattgat ttcttctcca aagaacccca atatttgcag
      121 taattgcacg ttctaatggt attgaacaga gattttctgt attttgaaat atttctttat
      181 acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa agttgatttg
      241 agtgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc ttatagtgat
      301 aagaaccttc agataagatt acaaaaggta cattttttaa catataattc gatataatag
      361 gatgttttgc caattcattt ttgggatttg taactaagta taattctgtt ttatagattg
      421 tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa aaatcaagtg
      481 tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt ttaagctcta
      541 tatctggata aattacatta aatctaggaa gtatttttga aagcaaaaac gtacctaaaa
      601 ctgtaggaat acctattttg agagaagctt ttctcttttt tcctaaatca agtgcgtcac
      661 attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa atttttcctt
      721 caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca agttcttttt
      781 ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt tccgcagcct
      841 tagtcatgtt tttatattta cagacagttt gaaaatagta taaatagttt atattcattt
      901 ggtattagtt cctttcattt acatattagt gacaagtata taattaacaa tagcataagt
      961 tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata agtttttgat
     1021 attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat agctggtatg
     1081 gtttagagta aatcaatcta tattacagtt atatcaacga atatcaaaaa attatctatt
     1141 aaaaaataaa aattattttt acaattaaat tgttaattaa atcataaaga aaaaagtgat
     1201 attaacaaga taaaattttt ttaatcagaa atgttaattt aataacaaaa tagagtattg
     1261 gggagaaagg agaaatatat tttttaataa tatttgttaa tttaataaca aaaatgaaag
     1321 ggagtgattt agtgaagttt gaattgatgg attttttaat gaatcctttt gtccttatgt
     1381 ttgctgcagt aattacagga attttatttg gaaaaattaa atttgggaag tttaattttg
     1441 gggtatcagg agcattattt acaggattat ttattggatg gcttgcatat agcttgggga
     1501 atctaataat agcaaaaggg gaaacagcag caggatataa ggcagcaact gtaatgatgg
     1561 ggaatggaat tatttcttct gacttctttg atttcttttt aataatattt gttgcagcag
     1621 ttggtctatt ggcagcaaaa gatatgaaag ctgtattaaa gaagtatgga gcaagatttg
     1681 taattttagg agttcttata acttttatag gtggctctat gacttatgca atgactttac
     1741 tatcaagtga taaaggaagt agtgcttatg aagtttctgg cgtttacaca ggagcattaa
     1801 caagttcgcc tggattagct gctgctttag aaacagctgg taaacatgca gaagatgtat
     1861 caaaagaatt tgaaaaagca tcaataaaag ataaaaaaga aattttaaaa gtagtcgacc
     1921 cagaaggcaa attggatgtg aatacaacta catcactgac tcaagagcaa atagataagt
     1981 atattgcata tgctgaagct ggtgttggta taggtcatgc agtcgcatat ccttttggag
     2041 ttttaatagt aatcttaggt gtaaatttcc ttccaaaatt atttaggatg gacctaaaag
     2101 aagaaagaag aaaatatgag aaggaaatga aggaagcaag agatagtgtt tctggtaaaa
     2161 cgataccaga agttccattt aatataataa catttttctt gacatgttta gctggatact
     2221 tagttggagg aatacatgta tttatgggac cactagggta cttcacacta ggtgcaacag
     2281 gaggttcact aattgtatct ctagttcttg gatatatagg taagataggg gtagtaaatt
     2341 tcagaatgga agaaaaggtt ttaaatattt taaaacaaat agggctagta tttttcttag
     2401 caatagttgg attaagatat ggtggaaaag tagttgattc aattatgaca tctggtatgc
     2461 acctagcctt agtagcaata gcagtaggtg ttacagctat gatgatagga ttcttagttg
     2521 gcaaatacgt atttaaatta aattggattt tattatctgg agctgtctgt ggtggtatga
     2581 catcaacacc aggtcttgga gctgctgttg atgctcttga tagtgatgac ccagcagcag
     2641 gttatggcgc tacgtatcca tttgcattat taactaaagt tatattggtt atagttttac
     2701 acaaacttcc aatgtaaata agattttata tggggggata tttatgaata atgaaggagc
     2761 atttgaagaa ttaagtggaa gattaagaaa taaagaattg tgtgatttaa taatgaatgc
     2821 agatgaagct gctaaaatta tagaggatgg catggttatt ggagttagtg gatttacacc
     2881 ttcaggctat ccaaaagcag taccattagc agtttcagaa agagctaaaa gtggagaaaa
     2941 gataaaatta accgtatatt caggagcatc tttaggtcca gaagttgatg gagcatggtc
     3001 agaagcaggt ataattgaaa gaagacttcc ttatcaaact aattcaatac ttagaaataa
     3061 tattaataaa ggtgttgttg attatataga tatgcaccta agtcattcta ctcaattttt
     3121 aaattatgga actataccta aagtagatgt agctatagta gaagcacttg caataactga
     3181 agaagggaat attataccaa ctagtgggat aggtaattct ccatctttta taaaaagtgc
     3241 agataaagta atagtagaga taaatcttgc aaaaccaatg gaaatggaag gtatggcaga
     3301 tatatatatt acggaaaatc caccaaatag aaaaccaata gaaataaatc atcctaagga
     3361 tagaattgga acaacatata taccttgtgg tttggataaa attgcaggag tagtaattac
     3421 caatatgcaa gataagaccc gtccattggg ggttgtagat gaagcttcga aaaaaatatc
     3481 aaataatatt attgcttttt taagagaaga agtaaaggca ggcagattat caaagaattt
     3541 acttcctctt caatctggtg taggaagtgt agcaaatgca gtattatatg gattatgtga
     3601 atctgaattt gaaaatttaa cttgttatac agaggttgta caggactcta tgttggattt
     3661 aataagaatg ggtaaagtta caatggcatc tactacatct gtaagtccat ctccagaagg
     3721 attaataaaa tttgaaaaag atattgattt ttttaaggat aagattatat taagacctca
     3781 agaaattagt aacaatccag aaatagccag aagaattggt gtaattgcaa tgaacactgc
     3841 aattgaagtt gatatttatg gaaatgtaaa ttctactcac ataatggggt caaagatgat
     3901 gaatggaatt ggtggttctg gtgattttgc tagaaatgga gcaataacta tatttagtac
     3961 tgagtcaatt gcaaaaaatg gtgatatatc atcaatagta cctatggtat ctcacgtaga
     4021 ccacactgaa catgatgtaa tggtaattgt aacagaacaa ggatatgctg atttaagagg
     4081 tcttgcacct agagaaagag ctataaaaat aatagaaaat tgtgcccatc cagattataa
     4141 agaacaactt agggattatt taaatagagc ttgtaaaagt ggagcaaaac aaacacctca
     4201 catattggac gaagctttat catggcatag taaatttatg agtacaggta caatgaaaaa
     4261 ggctgaaact tttaaaagtg cattataaaa tatttaataa attggaggta gattttaaat
     4321 ggaaaaagca gtggaaaact ttgaagattt aagtaaggaa tacattaatg gttatattga
     4381 gagagcaaga aaagctcaaa gagagtttga atgttatact caagaacaag tagataaaat
     4441 agtaaaaata gttggaaaag tagtgtatta taatgctgaa tatttagcta aacttgctgt
     4501 agaagagact ggaatgggag tatatgaaga taaggtagct aaaaataaaa gcaaagcaaa
     4561 agttatatat aacaatttaa aagataaaaa atcagttggt ataattgata tagatagaga
     4621 aactggaata acaaaggtag ctaaaccagt tggagtagta gcggcaataa ctccttgtac
     4681 aaatccaatc gttacaccta tgagcaatgc aatgtttgca cttaagggaa gaaatgcaat
     4741 tattatcaca cctcatcata aagcaattgg atgtagtaca aaaactgtag agatgattaa
     4801 tgaagaatta gaaaaaatag gagctccaga aaatttaata caaatacttg accaacaatc
     4861 aagagaaaat acaagaaatt taatttcatc agcagatgta gtaattgcca ctggaggaat
     4921 gggaatggta aaagctgcgt actcaagtgg aaaaccagca cttggtgtag gggcaggaaa
     4981 tgttcaatgt atcatagata gagatgtaga cattaaagaa gcagtaccta aaatcatagc
     5041 aggaagaatt tttgataatg gtatcatttg ttcaggtgaa cagtcagtaa tagtagcaga
     5101 agaaatgttt gataaaataa tggatgaatt taaaaataac aaaggtttta tagttagaga
     5161 taaagtacaa aaagaagctt tcagaaatgc tatgtttgta aataaatcta tgaataaaga
     5221 tgcggtaggt caatcagttc atacaattgc aaaaattgca ggtgttgaga taccagaaga
     5281 tacaaaaata atagtaatag aagctgatgg accaggagaa gaggatatta ttgcaaaaga
     5341 aaaaatgtgt ccagttatat ctgcatacaa gtataaaagt tttgaagaag gtgtagctat
     5401 tgcaaaagca aatttaaatg tagaaggaaa aggacatagt gtatctatac attcaaatac
     5461 agttaaaaat atagaatatg caggagaaaa tatagaagta tcaagatttg ttattaatca
     5521 atgttgtgct actagtgctg gaggaagttt ctttaatggt cttgcaccta ctaatactct
     5581 aggttgtggt tcttggggaa ataacagcat ctctgaaaat cttgattata agcatctaat
     5641 taatatttct agaattgctt attatatgcc agaaaatgaa gtccctacag atgaagaact
     5701 ttggggataa aatttagaca aaaaatattc attctaaatt aaagtaggaa tatattttaa
     5761 tttagaatga atttaagaca taaattaaat ttaaattagt ttattggact aaatttaaaa
     5821 tattaagaga catatagaag aaggaagtat tataatttaa aataaataaa aaaatctata
     5881 aaagagaagg agtggtttta ttatggcatt aatgacagga gctcaatata ttgaaagttt
     5941 gagaaaatta aatactaagg tttatatgtt tggtgaagag gttaagaatt gggttgacca
     6001 tcctatgatt agaccatcta taaattgtgt agcagcaact tatgacttag ctcatgaccc
     6061 agaatatgca gatttaatga cagttacttc aaatatcaca ggtgaaaaaa taaatagatt
     6121 tggtcatctt catcaaagtg ttgatgattt gataaaaaaa gtaaaaatgc aaagactatg
     6181 tggtcaaaaa acagcttctt gtttccaaag atgtgttggt atggatgcgt ttaatgcagt
     6241 gtattcaaca acatttgagt gtgataaagc ccatggaact aattatcatg ataattttgt
     6301 aaaatactta acttatatac aagaaaacga tttagtagtt gatggagcta tgactgaccc
     6361 taagggagat agaagtctat cacctagtgc tcaacctgac ccagatatgt tccttcatat
     6421 agtagaaaga agagaagatg ggataattgt aagaggagca aaggcacatc aaactggttc
     6481 aataaattca catgagcacc ttataatgcc tactataagt atgactgaag cagataaaga
     6541 ctacgctgta tcatttgcag ttccttcaga tgctgaagga gtgtttatga tttatggaag
     6601 acaatcttgt gacactagaa aattagaaga aggtgcagat gttgatttag gaaataagga
     6661 gtttggaggt caagaagctt tagtagtatt tgataatgta tttgttccaa atgatagaat
     6721 tttcttatgt ggagaatggg atttctctgg tatgttagta gagagatttg caggatacca
     6781 tagacaaagt tatggtggat gtaaagtagg agtaggtgat gtaataatag gagcagcagc
     6841 tttagcagct gattataatg gagcaaacaa agcttcacat ataaaagata aactaataga
     6901 aatgactcac cttaatgaaa gcttatattg ctgtggtata gcatgttcat cagaaggtca
     6961 caaaacagaa gctggaaact atcaaataga tttacttctt gcaaatgtgt gtaaacaaaa
     7021 tgtaactaga tttccttatg aaatagtaag acttgcagaa gatatagctg gagggcttat
     7081 ggttactatg ccttcagaaa aagactttaa atcagacctt aaagttggaa caagtggaat
     7141 gactattggc gaagtatgta acaagtattt taaagcttct tcagtggctt caacagaaga
     7201 aagaatgaga atattaagat tcttagaaaa tatatgcctt ggttcatctg ctgttggtta
     7261 tagaactgag tcaatgcatg gggcaggttc accacaagct caaagaataa tgatttcaag
     7321 acaaggaaac ataaatcaaa agaaagaatt agctaagaaa attgcgggaa taaaaaaaga
     7381 ggaagcttta aatttataaa aagttgatat ataaagggaa attatatatt aatgtgtgtt
     7441 aattaatgaa aaaatatgta gtataagaga tagttataaa attatggtgg tttttagcta
     7501 agtaaagaaa tttttaataa ggagaattta cacatgacct gtggagttag attttgtggg
     7561 ggctgtaacc ctagatacga aagagggaag gctcttacaa taatcaaaga gcacttcaat
     7621 ggtagggtag attttagtat agcacaagag aacgtcgagt atgatttgtt acttataatt
     7681 ggaggatgcg caactctatg cgcatcctat caacaatata aagtgaaaaa tgaatgtgtt
     7741 aagatgtggg attttagcca tatagaaaga gttataaatg tgattgaatc agcaatttgt
     7801 gaaacttaaa aaagctaaat ggggggattt cattgagttg gcaagaattg tatcaaagta
     7861 aattatgtag tgctacagaa gctgtaaaac agattaaaaa cggtgatact gtagtatttg
     7921 cacattgtgt aggtgaacct cctgcactag tagaagctat gattgaaaat gctgaacaat
     7981 ataaagatgt tgagattaaa catatggtaa gcttgggtag tggtggatat actgctaagg
     8041 gaatggaagc tcattttaga gtaaatccaa tgtttgtaag tggaaatgta agaaaggcaa
     8101 ttgaaaatgg agatggtgat tttacacctg cattcttcca tgaagtacca aagttgctta
     8161 gagaaaaaag attaaaatgt gatgttgttt tagcacaggt aactccacca gatgaacatg
     8221 ggtattgtag cttaggaaca tcagttgatt atacttatga agccattaaa actgctagaa
     8281 cagtaattgt tcaagtgaat gaccagtttc ctagaaccta tggtgaggta gtccatgtat
     8341 cagagtttga ctatatagtg gaaaaatcac aacctttatt tgaactacaa cctgcaaaga
     8401 ttggagaagt agaagaagca attggtaaaa attgtgcttc attaattgaa gatggctcta
     8461 ctttacagct agggattggt gggataccag atgcagttat gttattttta actgataaaa
     8521 aggatttagg gattcattca gaaatgattt cagatgggac tttggccctt tatgaaaaag
     8581 gtgttataaa tggtaaatat aaaaattttg ataaagaaaa aatgacagtt accttcttaa
     8641 tgggtactaa aaaattatat gactttgcaa ataataaccc tgcagtagag gtaaaaccag
     8701 tagactatgt gaatcatcca gcaattataa tgaaacaaca taagatggtt tctataaatt
     8761 ctgctataca agttgattta atgggacagg tagttgcaga ggctatggga cttagacaat
     8821 ttagtggtgt tggaggtcaa gttgacttta tacgtggagt gtctatgggt gaagatggaa
     8881 aggcaattat agctatgcct tcaataacta caaaaaaaga tggtacagta atttcaaaaa
     8941 tcgtatctat tgtggatgaa ggtgcaccta taacgacatc aagaaatgat gttgactata
     9001 ttgtaacaga atacggtata gcagaattaa aaggaaaatc tcttagagaa agagcaagaa
     9061 atttaataaa tatagctcat ccaagtgtaa gagaaagtct agcagtagaa tttgaaaaga
     9121 gatttaagga gaaatattaa taatagaggg gtgttaataa tggaagcact tagagtagtt
     9181 ccaaagatat tttactttga tacatttaaa gaatttaatg aagaatttaa aataggaaaa
     9241 aatgatttag taattactaa tgagtttata tatgagccat atatgaaacc tcttggaata
     9301 gatacaaatt taatttttca agaaaagttt ggaacaggtg aaccatctga tgaaatgata
     9361 gactccatga caaaggaaat gaagaaatac aattttgata gaattattgc atttggagga
     9421 ggaacaatag ttgatatatg taagatatta gcacttgatg ttccagaaaa gtctattgat
     9481 ttatttgaag gagaagtatc acctaaaaaa gtaaaagagt tagtagtagt tcctacaaca
     9541 tgtggtacag gtagtgaagt aacaaatgta gctattgcag agttaaaatc taaacatact
     9601 aaaaaggggc ttgcagtaga agaaacatat gcagactatg cagtgttaat tccagagaca
     9661 gtaaaagggt taccttacaa attctttgtt acaagttcgg tagatgctct tatacatgct
     9721 attgagtctt atctttctcc aaaagcaagt ccatttacag agatgtattc tctacaagca
     9781 ataaaaatga ttatggatgg gtataagaag atagttgata aaggagaaga agagagattt
     9841 aatcatctta gagactttgt acttgcatca aattatgcag gaattgcttt tggaaatgct
     9901 ggttgtgcag cagttcatgc actatcttat tcaataggtg gagctttcca tgttgcacat
     9961 ggtgaagcaa attatcagtt ctttactgaa gtatttaaga tgtattctag aaaaaaacct
    10021 aatggaaaaa ttataaaatg tacaaaaatt ttagcagatg cccttgaatg tgaccctaac
    10081 tgtgatgttt atggagaatt agaaaagttt ttaaataaat taatagctaa aaaagctttg
    10141 agagaatatg gaatggttga aagtcagata gatgaattta cagatagtac aatagcaaat
    10201 caacaacgac tacttgctaa taattatgta gaacttagta gagaggaaat aagagagata
    10261 tttgcaaatc tttattaata caatttttta tttatatgta tagtcaacat aaacaatgct
    10321 aattatattt atgtacaaat aaaaataagg gaacttaaat tttaattaaa gttaagttct
    10381 cttattttta tatggagtta aatataagta tatttttaat ttaaaatatg cttatattta
    10441 agtctattac tttatttgaa acaatttgta gaatttcaaa ttccttgaca gtaataagta
    10501 agtaattgta taatcaaact gcaatatatt aataatatta gtgaaaatga aaggattata
    10561 aaatatgtat aaatcagaaa atttttcaaa aaaatctgta ttaataaatt catttttaag
    10621 taaattttta aaaataattg gatggggttt ggtaagctta tttacattat cagccttagt
    10681 actttcaact atttccatta taattggatg ggctgtaata cttcctattc aagtatgtgc
    10741 agtattttta cttataggag gagtttgtat ttttaaaggt aagagattag gagaccaaat
    10801 ttctcgttat aaaaaatatt gtacaattat aaacaataga aatattattc cagtagaact
    10861 tattgcagaa aacacatcta aatcattaaa ttttataata aaagatgtac aaaagatgat
    10921 agacaaaaac tactttataa atacttatat tgataaaaga aataatcaga ttgtgttaac
    10981 aaatgaagat tttattccac caagatatga agatgtagca tatgaagtca aagaagaaga
    11041 aaagactgat gaatccaatg aagttgatac aattataaat aaaggaatga attatctaac
    11101 acaaataaaa gaagctaata aaaatattaa atcagaatca atgggcaata aaattgtaca
    11161 agtagaagat gttacaagta aaatatttga tgttgtaaaa catgacccat caaaacttac
    11221 tcaaatacaa aaatttatgg attattatct tccaaccact ttaaaactat taaattcata
    11281 tcatacattg gaagaacaag gaattgatag agaaaatata acaacaacta tggaaagtat
    11341 tgaaaataca atgaatacga ttgtagtagc ttttgaaaat caattggact atctgtttga
    11401 agatgaagcc attgatattt ctacagatat tactgtttta gagaatatgt tagtccaaga
    11461 aggattaacg agtggtaaat tttaacttca atagaaaaaa tattctgtta tttttaaaca
    11521 attaattagt cataaagtat aaaatgtgta ttatataagt taatcagtac tttatgaagg
    11581 aagaggagag aaagatacaa tgaataattt agatgatata ccagttatgc caactttgac
    11641 acttgaccca tttggagaag aatcaaacac taatgatata gataattctg acttattaat
    11701 gaaaaaggat gaaaaagacc cagaagaaga aaaactgtct gaatctgaac gcaagatggt
    11761 aaaggaattt gcggaaaaaa tagatattac aaatacaaat atgattttgc aatatggagc
    11821 tggtgctcaa aaaaaggtag caagtttttc tgaaacagct ttaaagagtg taaaaacaag
    11881 agatttagga gaagttggag atatgcttac aaatctagtt actgatttaa aagcattttc
    11941 agcagatgaa aaagaacaaa gtggtttttt aggaattttt aaaaaggcaa ataataaaat
    12001 ttcaaattta aaaactaagt atgatagtgc agaggtaaat gtggataaag taagcaaaga
    12061 gcttcaaaag catcaggtta aaatattgaa agatatagca atgcttgata agatgtatga
    12121 attgaactta gcttactcaa aagagcttac tatgtatatt atagcaggaa aacaaaaatt
    12181 aaaggatatg aaagaatatg agatgccaaa gttgagagaa aaagctcgtt tatctggttc
    12241 aacagaagat gctcaatctg taaatgatat ggtttcactt tgtgacagat ttgaaaagaa
    12301 aattcatgac ttagaattaa ctagaatggt atcgcttcaa atggctcctc agataagatt
    12361 ggttcaaaat agtaataatc tgatggcaga aaagattcaa tcaactatag taaataccat
    12421 tccattatgg aaaaatcaaa tagtgcttgc tcttggaatt tctcacttaa atcaagctat
    12481 gaaagcacag cgtgaagtta gtaatatgac aaatgagctt ttattaaaga atgcagaaac
    12541 tttgaagatg ggaacaatag aaacagcaaa agaatcagaa agaggaattg tagatataga
    12601 cacaattaag aaaacgaatc aatctctaat atctactatt gatgaggtag ttaaaataca
    12661 acatgatggt agaataaaga gacaagaagc agaagttgaa ttatcaaaaa tagaaaatga
    12721 acttaaaagt aagcttcttg aatttacagt taaataaaaa ttatactaat ataaaaagag
    12781 actgtctcaa aatagagata agtctctttt tcattgttta aatgattaaa gtatatttat
    12841 attataaatt tctattatct tttaacataa catcatgagc tccaccttca actatactag
    12901 tagaagatac aagtgtaagt ttagcatttt tttgaagttc aggaatgcta agtgctccac
    12961 agttacacat agtagaacga actttactta aagaaagagt tacattgtct ttcaaagaac
    13021 cagcataagg cacataagag tcaacacctt cttcaaaaga aagtttctta tctccaccaa
    13081 ggtcatatct ttgccagttt ctagcacgag cagaaccttc tccccaatac tctttcatat
    13141 aactaccatt tatattaact ttgttagttg gactctcatc aaaacgagag aagtatctac
    13201 caagcattat aaaatctgca cccatagcta aagctaaagt tatatgatgg tcataaacga
    13261 taccaccatc agagcaaatt ggaatataaa tacctgtttt ttcaaagtat tcgtctctag
    13321 cttgagcaac ttcaattata gaagtagctt gtccacgtcc tatacctttt tgttcacgag
    13381 ttatacatat agaaccacca ccaataccaa ctttaacgaa gtcagcacca gcttcagcta
    13441 ggtaaaggaa tccttcgcgg tcaactacat ttccagctcc taccttaact gtatcaccat
    13501 atttttcacg aatgaaatca agtgttattt tttgccactc agaaaaacct tctgaagaat
    13561 ctatacataa aacatcagca ccagcttcaa ctaaagcagg aacacgttca gcaaagtctc
    13621 tagtattaat accagctcca actacatatc tcttagaaga atctaaaagt tcaagtggat
    13681 tttctttatg agaagagtaa tctttacgga ataccatata cataagatta ccattatcat
    13741 caagtacagg aagagaatta agtttgtggt cccatataat gttgtttgct tcctttaaag
    13801 taacatcttt gttagcataa actatagatg acataggagt cataaattca ctaactttag
    13861 tatctaaatc catacgactt atacgataat cacgacttgc aacaataccc aataatttac
    13921 cagttgaagt tccatcttca gtaacagcta ctgtagaatg tccagttttt tcttttaagt
    13981 caagtatgtc ttttaatgtg ttatcaggtt taatatttga atcacttaca acaaaaccag
    14041 ctttgtggga tttaacacgt gctaccatag cagcttcatt ttcaatagtt tgagaaccgt
    14101 atataaatga aataccacct tctttagcaa gtgcaatagc cattttgtca tcagaaacag
    14161 attgcataat agcagaagtc aaaggtatat tcatataaat atctgcttcc tcgccctttt
    14221 taaatttagt aacaggtgtt tttaaactta cattagcagg tatacaatca gtagaagaga
    14281 aacctggtac aagaaggtat tcactgaaag tatgtgatgg tgtatcaaaa taaaacgcca
    14341 ttttttaatc actccttata tttttatact ttttataatt gatagtatta attataactt
    14401 attctttaat tttattaaag tattttgtca aatattgaga tttttgaaaa atttaaacta
    14461 ttttaattaa atttgtttaa tatatattgc cttatagggt aaagttgtat attaaataat
    14521 gtttagtgtt taatagtagc taatttaaaa ttaatcttta gtgattcata taattacatt
    14581 tgtatagttt atattgataa t
//
LOCUS       DAKBNN010000093        14431 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000093 DAKBNN010000000
VERSION     DAKBNN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.093"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     regulatory      complement(425..508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(741..1574)
                     /locus_tag="OUB02_002299"
     CDS             complement(741..1574)
                     /locus_tag="OUB02_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8886056.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNSGDSGDSSKVSVSGSTSI
                     GPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSAGLK
                     ETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREEGSG
                     TRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSLKIN
                     DVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFIKVN
                     Q"
     gene            complement(1826..3610)
                     /gene="pepF"
                     /locus_tag="OUB02_002300"
     CDS             complement(1826..3610)
                     /gene="pepF"
                     /locus_tag="OUB02_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCT8886057.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KIIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3676..4341)
                     /locus_tag="OUB02_002301"
     CDS             complement(3676..4341)
                     /locus_tag="OUB02_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCT8886058.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4492..4653)
                     /locus_tag="OUB02_002302"
     CDS             complement(4492..4653)
                     /locus_tag="OUB02_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HCT8886059.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4731..5240)
                     /locus_tag="OUB02_002303"
     CDS             complement(4731..5240)
                     /locus_tag="OUB02_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCT8886060.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5446..5664
                     /locus_tag="OUB02_002304"
     CDS             5446..5664
                     /locus_tag="OUB02_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCT8886061.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5682..7628
                     /gene="feoB"
                     /locus_tag="OUB02_002305"
     CDS             5682..7628
                     /gene="feoB"
                     /locus_tag="OUB02_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCT8886062.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7807..8862)
                     /locus_tag="OUB02_002306"
     CDS             complement(7807..8862)
                     /locus_tag="OUB02_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8886063.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8910..9710)
                     /locus_tag="OUB02_002307"
     CDS             complement(8910..9710)
                     /locus_tag="OUB02_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT8886064.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9703..10494)
                     /locus_tag="OUB02_002308"
     CDS             complement(9703..10494)
                     /locus_tag="OUB02_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8886065.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10534..10956)
                     /locus_tag="OUB02_002309"
     CDS             complement(10534..10956)
                     /locus_tag="OUB02_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCT8886066.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10972..11451)
                     /locus_tag="OUB02_002310"
     CDS             complement(10972..11451)
                     /locus_tag="OUB02_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8886067.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11583..14339)
                     /locus_tag="OUB02_002311"
     CDS             complement(11583..14339)
                     /locus_tag="OUB02_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886068.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4712 a         2485 c         1636 g         5598 t
ORIGIN      
        1 aatattgtat taaacattaa gattcattat taaatactaa ttaaagtctc cgattgttgg
       61 aaaagggaaa tttttttaaa agaataatag tattataata agaaaataaa gtttttataa
      121 acattgagat gcagaatata aaccttttgt aacaatatat ttaccaaaat aatacaattt
      181 attttatatt ataaccatat aaatcatata aatcaatact caaactagcc aatagacaag
      241 tttctagaaa aataataaat ttatacgtga aaatgacttt aagctccatt ctccaacaaa
      301 tggctacttt aaaagccaaa attttaccta tcaataaaaa tcttaacgac taatactcct
      361 attttcaaat tttgcaacat atttgttaat tttaaaacaa aaaaagacta tgcaatataa
      421 aaattatatt catagccggt tgcaccacta actccatata tctctagatg cccaataata
      481 agatatactt ctcagtttct aaaatatttt tattaaaaat cttttatata ttttcataaa
      541 tttctttttt attctaacat tgtaatatta ttttgtcaat atttccatat tcttagagat
      601 aagatactaa aatacctatt ttatctaaag atatttatat ttctacattc aataatagac
      661 aataccctgc aagttaaagc atcattttta tgagcttatc tcacagggta ttatatgttt
      721 actttatatt ataactcttg ttattgatta actttaataa atcctttttc tgaaacaagt
      781 gcttgtcctt catcacttaa tatataatct attaaagctt tacctttttc tgacaaacca
      841 tcttctttat taactaatat aaatggtctt gatattggat atttattagc tattacattt
      901 gcatcagttg cttcaacatc atttattttt aaactattta ctttctcatc aacatatcca
      961 aatgatatat atcctatagc attttcattt ccttcaactg tagtttttat atttcctgaa
     1021 ccatcactta tttgtgcatc tttagttagt tcttctgaat caaaccctac aatttcttgg
     1081 aatccatctc tagttcctga accttcttct cttgatacta ttactattgg tgcatcattt
     1141 cctcctactt ctttccagtt agtaatcttt cctgtatata tacctttaac ttggtccatt
     1201 gtcagatttt ttactgagtt tgcagtatta gttatcaatg ctataccatc tattgctatt
     1261 ttagtttcct ttaatccagc acttttttct tcatctttta aatctcttga actcattcct
     1321 atttcagatg ttccttctat agcatttttt atacctccag aagaaccaat ctgttgtact
     1381 tctacactaa caccttgatt tttttcttga aacttttctc cgatagtttc cataagagga
     1441 cctattgaag ttgaacctga aacagaaact ttgcttgaat ctcctgaatc tcctgagtta
     1501 gagccacatc ctactgctaa agctccaact aacattgtac tagctaaaag aacgcctatt
     1561 ttctttttaa acattttact tacccccgct tatattttaa ttttttgttg tcatttttct
     1621 atattttaat aatacagatt ctttgttaaa tgaatattag agcagggtta agtaaatgta
     1681 aatattgtta aatacatgta aaaacttaat tttatatgat ttattcttaa ttagattaac
     1741 tttttaaagt tactttaggc agatataaac ataatacaac tcatattatc agaagtcttc
     1801 attgaagcta tcaacctatc acatcctata attctttttc tagtttatta actaattcag
     1861 caaatacatt taaggcttcc tctatagatt cttttttcgt catatctaca ccagcagatt
     1921 ttaattgttc tagtgggtat tctgaccctc cactttttaa gaagtttata tatctatcta
     1981 ctgcagtact tccttctgaa agtatctgtt gacttaaagc acttgcagca gaaaaccctg
     2041 tggcatactt atatacatag aagtttgaat aaaaatgagg tattctcgcc cattctattc
     2101 ctatttgttt atctactatt gcagattttc cataatactt ttcatttaat ttgtagtata
     2161 tattagtaaa ttcatcagca gtaagtggtt ctccactctc taccctttga tgtacaattt
     2221 tttcaaattc agcaaacata gtttgtctat gaactgtagt tctaaattgt tctaaataat
     2281 aatttaataa gtatatagtt tcatccttag acttagaatt ttctaataaa taatttataa
     2341 gaagtaattc atttaatgta gatgcaactt ctgcaacgaa tatcttatag tccgaatata
     2401 aaaatggttg acttgttctt gaataatgac tgtgaacaga gtgccctaat tcatgaatta
     2461 aagtgaatag agaattaagg tcatttttat agctcattaa tatataagga tgtgaatcat
     2521 aacttcccca agaataagct cctccttttt taccttcatt atcatataca tcaatccacc
     2581 cttcatcaaa agcacttttt atgatcttca agtattcttc tcctaaaggt ttaagtgcct
     2641 ctaatatgat atcctgtgct ttatcatatg gtatatccat gtcaaattta tctgtcaaag
     2701 gaacatacaa atcatacatg tgtatttcat tcaatcctaa aaatttcttt ttaagctcaa
     2761 catatttatt tagattagga atacttttat ctatagctga tataagatta ttgtaaacat
     2821 ctaaacttac atcatctgaa aataaagacg cttctattgc tgaattatac tttctagttt
     2881 ttgcgtaaaa tatctctgat tttattccac cataaagcgt tgaggcaaat gtatttttat
     2941 atttctcata tgttgaaaat tcaccttcaa aagcgtcttt tctaactcta ctattcttac
     3001 tttttataaa taaagagtag tttgaatgag aaagcttaac cttattcccc tcctcatctt
     3061 ctatctcagg aaattccata tctgcatatg ctaacatatc gtatacattt tctggaattg
     3121 tcgataattc tgatgtagct gcaagtattt cttcctctcg ttcactcaaa gtatgtggtt
     3181 tatctctcaa tatatccttt atatattttg cataaaagga tagcttctca tcttttaagt
     3241 attcacttaa cttattctct tctatcgcca gtatttctgg aactatataa gaagtagcca
     3301 cacctaaatc agtagataac atttctgtct tagtggctct tccttgatta tcattaattc
     3361 tagtatcttc atgttgtttc atatgagtat atacatataa attttgaaca atcctagatg
     3421 cattttcaga gatatttaat actttatata agttctcctt actctcagaa agcttccctt
     3481 tatattcttt tacttcttgg ataagatttt ttactttact tatgtctttt tcaatttcct
     3541 ctttactgga gtacatctta tctatcttcc atttgtactt ttcttctatt gtacttctat
     3601 cagttcccat atcatacacc tctattttaa tttgttttat attttattca ctttacaaaa
     3661 tatattgtat atattttagt ccaaataatc tacatttatt actccttcta tcatatttat
     3721 ttctttcata acgtcgtgta agtgtattct cttagcataa gatacaaaaa aattaacctt
     3781 ccagtattct tcattacgac attctatttc catatttctt acgataagtt gatttttttc
     3841 aaatttatca tataaaaaag gtatagagtc caaattgctt aattttaata tcaacacatt
     3901 cttttcttgg ctaaataaaa acgtattgat tatcctaagc acaaataatg ctatcattac
     3961 aaataatcca cctagaattg ccaattcata ataaccatat ccaactgcta taccaataca
     4021 tgcagttgcc cacatcccag cagctgtagt aagccctttt actccacctt tattttttaa
     4081 aattgctcct gcacctaaaa aaccaattcc agataatact tgagcaggca ttcttgctgg
     4141 gtcagcattt gtatacaaat tataatcaaa gaataattga atcgaagtta ttgaaacaat
     4201 acaagaacct attgcaatta gtatatgtgt cctaaatcct gcaaactgtc ttattttttc
     4261 tctctctaag cctattattc ctcctattat tagtgctaat agtaacctta ttgctatctc
     4321 cttacttcct atatcaatca taatataaaa ccctcctgac ttaattacaa cttatgtcct
     4381 acagataatt tacacatcag aaatactatt ttatatctta atattattat taatatatgt
     4441 aaaatatagc catctgttaa tttttgatac aaaaaaaccc acaactgtgg gctatttgct
     4501 taggtacatg tatttattta ttaatatatc tattttctta cttgattcta ctaactcttc
     4561 ttgaaatatt tctggatatt ctatgtatgt atttatctct tccctaactg cttcaatctc
     4621 attttttaac gcttctaatt cattgttcgc cataataatc accactatta caaatttgta
     4681 tatttttata tacattttat caaacaatat tatattttgt caatattatg tcaataaaac
     4741 ttactataaa aatgctttaa tttaggtatt gtatacctgg atactaagcc tacaaacata
     4801 ccagttacta atgatgccat taacataaat ggtaagtacc ctatagcaag tatattttct
     4861 atgattaaac ttgcaactat taattgacct atattatgcc ctattgcacc tataatgctt
     4921 atccctatta gtgataaatc ttttatttta ctcgctaaat acataaataa aagactcaga
     4981 taagctcctc ctatactaaa tagtagatat gataaagggc ctgcaaatat tgatgacaaa
     5041 attaccctga tagtaacaat tataacagta tctttgacac ctaacaacat taatgaaatt
     5101 aaagttataa tattgctcaa acccaatttt gcccctggaa atataactat aagaggattt
     5161 ggtatatatg tctctaatac atataaagct aaactatacc ctatcataag acctaaaaat
     5221 gtcatttttc tagttttcat taagttaact cctccaaatt ttatctatca tcttacatta
     5281 taatacaaaa tatataaaaa cacctaattt agactaaaat atttttaatc tatttagaat
     5341 aaatattagt tatttcaatg tgcaattacc tctataaatc ttgacttttt gtatcatact
     5401 tgttacaatt ttaatgataa tgaatttcat taggaggtgt taataatgac tgtttacgat
     5461 ttaaaaatag gagaaaaagg atttattaat agtatagatg gagatattaa attatctaaa
     5521 agattgcttg cacttggatg tataaatagt actgaaatag aagttaaaaa ggttgcccca
     5581 cttggagacc caattataat tagatttaga ggctttgacc ttgcgattag aaaatctgat
     5641 gctaaaaata tttctttaca ataatagagg aggagctacc cgtgattaat gtagcattat
     5701 taggaaaccc aaatgtcggt aaaactactg tttttaatct acttacaggt tcaaatcaat
     5761 atgtaggtaa ctggcctggt gttactatag aaaaaaaaga aggatttctt ggaaaagaga
     5821 taaaggttgt cgatttacct ggtatatatg caatggatac tttctcaaat gaagaaaagg
     5881 tttctaaatc atatttagaa aatgaagatg ttgatgtaat agtaaatgta gttgatgctt
     5941 ctaatttatc aagaaacctt tacttaacaa ctcaacttat gcaatttaac aagcctatag
     6001 ttatattgct taatatgtta gacattgcag aatctaaagg agttaatata gatgccaaaa
     6061 aattaagtga agaacttgga gtaattgttg ttccaattat agcaaaaaag aaaaatggtg
     6121 ttgataggat agaagaagag attaagggag ctacgcaaaa agcttttata tatcagaaaa
     6181 actttggaag tgaaacagaa acatataaaa aattagaatc cattttatca agatgtacaa
     6241 aaactacata tacacaaaag aaatctatca gtgataagat agacaatata gtcttgaatc
     6301 ctatattagc atatccgata tttattggtg tattgttcct cttatttaaa tttacctttg
     6361 actgggtcgg aggtccacta caagagggat ttgctagtct tatagaggct tatatttctg
     6421 cacctgttag tgatatgtta tctaactcta gcccatggtt taaatcactt atagttgatg
     6481 gaataattgg aggtgttggt ggtactttac cattcttccc tttaatattt acattattct
     6541 ttggtatatc gttgtttgaa gatagtggat atatgtcaag aacagcattt ttaatggata
     6601 aagttatgag aaaggttggt ctatctggta aagcttttat acctatagtt atgggtatgg
     6661 gatgttcttc tccagctata atggcaacta gaacattgga aagcgaaaaa gatagaagag
     6721 ttacagctct tattgcacca cttatgactt gtggagcaaa gctacctata tatgcactat
     6781 ttgtagctat attctttcca cataatgctg ctttagtaac tacatcactg tatttggtgg
     6841 gtatagttat ggcaattgta gttgcattat tcttaaataa aacagccttt aaaacagagg
     6901 ctgaaccatt tatattagaa ttaccagaat atagaatccc tactatagat gcacttatta
     6961 aaaatacttg gaataaatca aaaggtttct tgataagagt tgttactgtc atgtttgcaa
     7021 tgtcagtcgt aatttgggga ctttcttcat tcaatgtttt tggatataca gaagatataa
     7081 atgtaagctt cttggctact ctaggtcata ttatatctcc tatattcaaa cctcttggtt
     7141 ttgatgattg gagaacttca gttgctattt taagtggtct tggagcaaaa gaaatcgtag
     7201 taaactcttt aaatatacta tatggtaatc taactgttgt cttaccaaca atatttaatg
     7261 gagttactgc atatacattc ttgatattta ctgctttata tactccatgt atagctgcac
     7321 ttgcaactat gaaaaaagaa tatggcaata aaatgatgtt tacatcattt gcatatcaat
     7381 ttatactagc atggataatg gcttttatag ttaaaagtat aggtggagta ctatttatgg
     7441 gtaattcaat aatagaagct ttgataggtg gaataattat agtccttgca ctctttattc
     7501 tgtttaataa attcaagtct acaaaaaacg gaaatgtctg tagtggatgt tctatttgtt
     7561 ctggatgtcc aagtgctaat tcttgctcaa gtaaggaaga cagcaataaa aaaaataatg
     7621 cacactaata tataaatatt ttagtttaaa ataagataaa agtgggtgtc tcaaaattaa
     7681 ctttttagtt catgaggcac tcttttttat agaaaatcaa atatattttc atcatttcaa
     7741 cagtaaaaaa gagcttatct agtaaaaaag aggcttatct ctattttgag acaacctctt
     7801 tttttgttac ttattcagtt tcttgctacc taacttcatg tggaacctag tatcttttgg
     7861 aacacttggg tctacacctc ttaatgtagg tatttcccca catattagtt gtataggtac
     7921 tataaaatta aatgtttgat agtaaggatt atctgtgata tcaatcttta aatttttatc
     7981 attttctgta acatctctac ctatagcaaa tacattttcc gtccaaccag aaagtacatc
     8041 aatcatctta gttactctag gttcttttcc tgtatctagt ataaatatag tagaatcact
     8101 atttatagca ttatatatac catgtataaa ctcttcaaac tcatacccag ttacaggtat
     8161 tctcattgtt tccaataatt ttaatgctgc ttctaatgta tctccatata tatcagagtg
     8221 acctattatt cttatttctt tagaattaac taacttttct ttgtttcttt caatccattg
     8281 tttagagagc ttatacactg cttcaaatct attaattgcg tctaatatct tatttatttc
     8341 ctcattgtac ttttcactag atattatacc tttttctcta gctatttgaa gacctaataa
     8401 cattaagttt aactttgtac aataatatcc ttttgtttta gctccagatt tttcttcacc
     8461 acaatttact gttaatatat aatcagaaat ctcatctatt aaagcattct tacaacctgc
     8521 catagatgct attttacagc ccttatcttc agctaacttc atagcgttgt atgtagaata
     8581 actactccca ccttgagata cacctactac tagggtattt tcattatcaa acttgaatgt
     8641 atcttcagtt atcataaatg gatacatttt cacaacatct atatctaata aattttgtaa
     8701 atatggttgc acttgcactc ctgagtgata gctagtacct gaacctgtta ttataatctt
     8761 ttttagattt gttctcttaa cttcattaaa taagctatct gcattagata taatttctct
     8821 cattctaact ggtgtttcta acatataatc ttgtattgtc attttataca ccttccttat
     8881 ctatatattc aataaattat aattaatttc tataacaaac ctatagcatg taaagcgatt
     8941 cctgttacta tagtgcctat agttaatttt gttacactta cctttctctt taacatgtag
     9001 tatattacaa atgcaaaggt taaaggtaac atattaggta ttattttatc tagtatatct
     9061 tgtaaaatta cttctgcacc atttaagttg aatttcagag gagtagttat actcaacata
     9121 gttgcaacca ttcctcctac aacgaataaa ccaacagttg tagccataga cattattttc
     9181 tccataagtc cctctctttg tattctttca agagaattta cccctacttg ataaccatac
     9241 ttaagtccaa atattctaag ggcaaatgct ggtatattgt ataatagtaa gaataatagt
     9301 ggtccaaaga tattaccttc ttttgcaagt gatactccta ctcctgctgc tataatccta
     9361 aatgttcccc agaataatga atcaccaatt cctgcaagag gtcccataag agctgtttta
     9421 acagcactaa ttgaactttc atcaaattct ggattattag cattctcttc ttccatagct
     9481 gctgttatac ctaatatagc aggaactact actgtcgttg tattaaaaat ctccaaatgc
     9541 ctttctattg cttttgcttg attttctttc ttattgtata gcttctttat tgcaggtaac
     9601 attgaataac agtaacctaa gttttgcatt ctttcgtaat taaatgcacc ttgaagagca
     9661 aatgaacgcc aaaacatact tcttaattct ttttttgtta atttattcaa agtcaatctc
     9721 tccttccgtt aaagtattga ttgctaattg aggattttga ttattttctt tgttattgat
     9781 ataaaagttt agtacaaatg ctacacatac tccaaataaa gctatagcag ttatatctaa
     9841 tccaccataa gatgctaaca aaaaccctat aaagaaaaat acagacatct tttttgaaag
     9901 taacatatcc ataagcaatg caaaacctac tgctggcaac aatgttcctg caacacccag
     9961 tccgtctaat ataactttag gtattgcatt tatcaatgct gtaactttat cagcaccaat
    10021 cataattgct aaaaaggttg gaactgatgt acttaaaaag aataatatta ctggcaacca
    10081 catcattatg gtaactttct taaaatcagc ttgtttagca tagaaaactg ccttttgaga
    10141 aaagtacgaa tttattattc taactaatac tccaagagat tgtgctaaga ctgctactgg
    10201 tactgctact gctaaagcaa cttctgcacc attacctgat aatatagcaa aagctgtacc
    10261 taatacgcct cctgttacta catctggagg agtagctgca cctattcctg caattcccat
    10321 ccaaattaat tcaagctgtg caccaatcat aattcctgat tttatgtctc ctaaaactaa
    10381 tcctactaac atacctgtaa ctattggcct atcaagcata gattgaccaa atatacgccc
    10441 atctagtata ccaactcctg ctataatagc taacatagta gcttgtacta acatattgaa
    10501 cctccatttt aacttagttt aatttacatt aaattacatt acatttttga ttttgttttg
    10561 attttctaat aactcattta cataaacaat tccatttttt gcttcctcta atcctgattt
    10621 aactattagt tttgtatcct cctcaactaa taaaagtata gataaaagca ttggtaaatt
    10681 tactcctgtc aataattcaa atttgaaccc ttgtaaaagt aagtttgtac atatgtttgc
    10741 cacacttcca ccaaaaacat ctgttaagac aacaaattct tcactatctt tattatttga
    10801 taatatactt ctaatttctt tctcagcatc atctgctgat ttatccgttg tcatacaaaa
    10861 atactcaata ttagttaatg aaccaattat taatttactt gtgtcaacaa gctctctgga
    10921 caattctcca tgtgttgcaa ttagaaattt tttcattctc tttctcctaa attattttat
    10981 taaatctgat agttgtttct ttttgtctct aggtaacatt ctaaattcta tatcaatccc
    11041 taaatcacct atttgtttaa agttatttat atcatcatca tcaacagcaa ctaaatctgt
    11101 aagtttcttc ttcccttcct catatcttaa tcctcctaca tttatttctt ttaatactcc
    11161 tcctgaattt ttagctacaa ctaaagcatc tttggtattt tttactaaca ctaatacatt
    11221 gcttttcttt gaagaagcaa atttttctac tatttctcca gattcctcta ccccccttat
    11281 atataaagtt acattgctag gtttcgctaa gttcagtgac atagctttca tttggtcatt
    11341 ttttgcttca tcatttgcaa ctagtattac atttatacct agtgatgttg cccatccata
    11401 tgcaacctga ccatgtatca atctgtcatc aattctcatc aaagatacca ttttccccaa
    11461 atcctccatc atacattaaa atttttattg tttatactct catttatcta taaaagcaaa
    11521 aagtatgcca aatactggca tactttttta aatttcattc acttattttg taatcttgta
    11581 ttttactgtg gtagaccata gataatatta taaatatatc ctatctctga aattggtatt
    11641 tgcacactat aactcttttc tagcacacta aatgcatttt ttattagttt tacttcacta
    11701 tgatggtttt tgattaacaa atccatatca gtatactctt caatttcagt ttgtctaact
    11761 aatctttcaa tcatacaact taagtgaaca tataaagaaa tctttttatc atttgaaagt
    11821 tttttctttt gaatattttc tatagcatta attccttctt ctattctctc tattatcttt
    11881 tttgaatcta aaatagttag actatctatt actctaatta atgaaaaatt ctttacaata
    11941 gaatcattta tgttctttat tttttcattt tctactactc tgctaaatat tctaaaaaca
    12001 tcatcttctc cttttcctga tattatatcc tctaataata taaaatctac cccattcaca
    12061 tttggattgg cagttcctac tatagccatc acatcataca tttcaaaaac tggttcttta
    12121 tctcccatag aaacaagact atcataatca taaggtatta ttttgacatc aactattcca
    12181 attaaacttt cggaaagtag atgttggatt tttatagcag tacctatccc tgtaaaacaa
    12241 gatgatatta tagcatattc tttatttttt tgaggataat acaattgtat attaggttta
    12301 gtttcttcat ccataatttc tgcaatctct tcaatgtttt taccttgcat taataaattt
    12361 cctacttcta gtgcagaaat agtggatatt tggtctataa ataacattgg gctatttatc
    12421 ttattcttta aatcttcata tataagatta agagagccca tatcaaataa cacaattaaa
    12481 ccattcttta tcctatctcc ctctaggtat ctagacattt gttttgatac atcagcaata
    12541 gtagcatcta taggcatatc aatagctgta aatatatttt tacccaacat ccgattacat
    12601 acattcgcca tactactggc agtagcatat ccatgtgcta taatagtcgc attaacttga
    12661 ttaaatttca ttcttataat tgttgattta ataaatataa tcattatgat tgtatctaca
    12721 ttattaagat acatatccaa tctattcgtt attaatgttg atattttact tattatagag
    12781 tattctttgt aactctcttt ttttaaatat attagtaatt tattttctaa agctttatac
    12841 tcatcagaca attgataaga ttcgtccttc aaataaagat aagacgaaaa tgccaataca
    12901 ttattcccat catattttat attgttatta acttctaaat ttctaaatat atcctgcatt
    12961 atcgatatta taagctccat aagtacgtta ttattttcat cattatttga aaagactaaa
    13021 ttgtccatta gtctaatcat tagattagaa ctctttttac taaatttttc ctttgttatt
    13081 tcattttttt ctagtctatc aaagtaatct aaacatttta tgaaactttg atagattata
    13141 tttcttttat ttcttccatt tgacatttca atactaaatt gtttaggaga aaagataatg
    13201 cttttgcttt taccactttg gaattgaaat atatctacat tttctatata aagatttcta
    13261 ggaaggtctt gaagagtaac aaaaatattt ccatttcttc tttgattttt tgcataagct
    13321 cgtccacatg catattttat tacattttta cattcaccta tattcccctt gaattttatc
    13381 tttaaaatcg catttaatgt aatttgagtt atctctatag gcaaaccaag taccttactt
    13441 tcttcataaa taaaatgttc tacaatcttt cttttttcaa tagtccctct ttcttctata
    13501 ttgggaacat aaatatttat gggtattctt cttaaaaagg tttgtaaaaa ttcagataga
    13561 ctttctgttg tagcaaatat taatcttacc ctagcttttc taattactcc attctctcca
    13621 atcctagaaa acgtacctgt atccataaat gtaaataact tttcctgacc ttcactattt
    13681 aatctatgta cttcatctaa aaataaaaca ccaccatctg attgttctat tagacctgct
    13741 ctagattcat ttgctccagt atatgctcct tttacatgcc caaataatag actagacaat
    13801 aactccaaat tattataata ctgcgcacag tttaacacat tgtatggaga atttttttct
    13861 aatacattac tgcttacact atatttgtat attatcttgg ctaaaaaagt ttttcctact
    13921 ccactttcac ctgtcagcat tattgtaagt ccgccatttg gataaagtac agcggttttt
    13981 atttgttcga ttacttcttt taagctattc tcatatccta tgatactatt aaacacagtc
    14041 tcttgcttta ataatttaca ctcattttta agttcatcta aattttcata aatacttttt
    14101 tttgtaggtc ctactacatc atttatttta tttttaggta aaaaaataac aggtctactg
    14161 tttatcttta taagattatc agattttact aaatcattta ataatctact tgattgagtt
    14221 ctacttaaat taaacttttt tactatatct tctgttatta tgccactttc ttttttaagt
    14281 tcatcaaagt tgttaaataa tacacattca tttacaaaac ttaaaatttc atccctcatg
    14341 ttcaacctcc aatacaaata tcaataatac tataatatta gtatatcatt tatatattta
    14401 tgaaaaagat aattatattc ctatcttgat t
//
LOCUS       DAKBNN010000094        14248 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000094 DAKBNN010000000
VERSION     DAKBNN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.094"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            275..445
                     /locus_tag="OUB02_002312"
     CDS             275..445
                     /locus_tag="OUB02_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886069.1"
                     /translation="MSALLYGVCNKPISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            988..1866
                     /locus_tag="OUB02_002313"
     CDS             988..1866
                     /locus_tag="OUB02_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCT8886070.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILKAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1902..3797
                     /locus_tag="OUB02_002314"
     CDS             1902..3797
                     /locus_tag="OUB02_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8886071.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4066..5973
                     /locus_tag="OUB02_002315"
     CDS             4066..5973
                     /locus_tag="OUB02_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8886072.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQKEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            5963..6412
                     /locus_tag="OUB02_002316"
     CDS             5963..6412
                     /locus_tag="OUB02_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8886073.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            6512..7399
                     /locus_tag="OUB02_002317"
     CDS             6512..7399
                     /locus_tag="OUB02_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8886074.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            7804..9252
                     /locus_tag="OUB02_002318"
     CDS             7804..9252
                     /locus_tag="OUB02_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCT8886075.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            9278..11668
                     /locus_tag="OUB02_002319"
     CDS             9278..11668
                     /locus_tag="OUB02_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCT8886076.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            11670..12341
                     /gene="pgmB"
                     /locus_tag="OUB02_002320"
     CDS             11670..12341
                     /gene="pgmB"
                     /locus_tag="OUB02_002320"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCT8886077.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKYDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            12380..13783
                     /locus_tag="OUB02_002321"
     CDS             12380..13783
                     /locus_tag="OUB02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8886078.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTIIGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIGKLRKCVK"
BASE COUNT         5836 a         1435 c         2484 g         4493 t
ORIGIN      
        1 taagataaaa tgtaataaaa tttaatggag aaattttata ggtagataaa ttttaatatt
       61 tataagatta tgttaaggaa acacttaaaa attagattaa aattgtgggt atttaaatca
      121 actaaaagat attgactatt tagtcaatat ctttttttat taaaaaggaa tataaagaag
      181 ttaggctaaa aattgggata aatatgattt atatagcatc atttaattaa aaggagctat
      241 attagtattc attcatacaa ttggataata tacaatgtca gccttgcttt atggtgtttg
      301 taataaacct atttcagttt ctattgtaat taatatgcaa gaatttaaga taggagtatc
      361 tatggcttat ggtgtattag taattggtat atgctatata gcattaggaa taatttttaa
      421 attagataga aaaagattta cataagataa caaaggctaa tttataaatt ggtgcttaga
      481 aaaccaaatc actttattag ctagagatga atttggaggg tgtcactttg gtgttttatg
      541 caaagtttat ggaagtaaaa cattagataa tcgtgagtaa gatttctata aatcgtatat
      601 actattggaa tccaagaaaa ggtattggcc aaatagctaa tatcttttta atttttttga
      661 ctctaatata gcattaacta ttgcatgagt atctttaaat ttatagttaa tgtatcttaa
      721 ttgtttatta taaaatctag gaaggctaaa gcataaaaca cttaaaaaga gaagatgtac
      781 atgagaaata taattaacta tgatgcagac ttctaaaaat tatttagtga aaaggaactt
      841 agagtgtata ataaaagttc cttttcacta aggaaaacga ttgttatttt ctgagcttca
      901 ttaccctgat attatttaaa tataaacaac aacaaaatta acaagaagca aattcaacct
      961 aaaatttaaa atatcgggag gtacaatatg ttaataaata tgaaggaaat gctaaaagtg
     1021 gcacaagaaa atcaatttgc tgtaccagca tttaatatag gaagtgggca aatacttaaa
     1081 gcagtagtac agagtgctaa cgaaaaaaat gcaccagtaa tacttgcaat acacccaaat
     1141 gagctaagtt ttctaggaga tagctttgta gctagttgca tagaagaagc aaataagagt
     1201 aaagtaccta tggtaataca cttagatcat ggggaaaata aagaacaaat attaaaggca
     1261 ataagatgtg gatttacatc agtaatgatt gatggctcac atttaccata tgaagagaat
     1321 gtagctatat ctagggaaat agtagaaata gcaaaaggat taaatgtgtc cgtagaagga
     1381 gaacttggaa caataggaac aacaggaaca tcaagtgaag gtggtactga tgaaataata
     1441 tatacagatt caaaattagc taaggatttt gttgaaaaaa caggagtaga aacactagct
     1501 attgcaattg gtacagcaca tggtatatat ccaaaaggtt ttaaaccaga gcttaaacta
     1561 gatttattaa aagaaataag agaagtagta gatatacctt tagttcttca tggtggttca
     1621 tcaaatccag atgaagaaat agcacaagca gttaaactag gagtttgtaa aataaatatt
     1681 tcgagtgatg ttaagtcggc atattataag aagtgcagag aactattaga acaaaaccca
     1741 agcttatatg agccagatac tatatatcca ccatgtataa aagctgcaag agaagttata
     1801 gagtttaaaa tgaatctatt caatgctata gataagttaa agtgctttta taaaaataaa
     1861 aattaattat gaggtaacaa attaaaagga ggggagaatt tatgataggt aaaatactac
     1921 gaagcgattg tattgtacta gataaacaat tacaaacaaa aaaagatgta attaattata
     1981 tggcaaatca tttgtaccat ggaaattata taaataataa agaaaagttt atagctgata
     2041 ttaataaacg tgaaaaagaa tcatcaacta atatgggtaa tttaatagct atacctcatg
     2101 ctaagtcaaa tgtagtaaat caacctgtaa tgatgtattt aagaacagat agagagatta
     2161 cagataatga tggtcaaaaa cttaggtcat tttttatgat agcagttcca gaatcaggtt
     2221 cagatgaaca tcttaagatt atatctaaac tagctacata tctaatggaa gaaaattttc
     2281 ttaagcatct actaagtgca gaaactccat acgaaatatt aagtatattt aataataaag
     2341 ctgcagaatc aggagatttt agtaataatc aaaataaagg tcttatagtt gggataacag
     2401 gatgtccaac aggtatagct catactttta tggcaaaaaa agcattagag gaagcaggtc
     2461 aagaacttgg atatgaagtt aaaatagaaa ctcatggagc tataggtgta gaaaataagt
     2521 tgactaaaga agatataaaa agagctgaat acgtaataat agcttgtgat aaagaagttc
     2581 aactgagtag atttatagga aagaaagtgc tacaaaagaa agtagtagat gctaagaatg
     2641 tttcattagc taaaaattta attataaacg ctgaaaatgg aatggctaaa gaaattaaac
     2701 caggtggaaa gactttagaa gattcaaaag aaatgcttca agggtcagag aaaaagggtg
     2761 tttataagca cttaatgagt ggtgtatctg aaatgattcc atttgtagtg ataggtggaa
     2821 ttggtatagc catagctttt atgtttggta tatttgcatc agacccaact catgaaacat
     2881 acaaccctat agctggattc tttggacaac ttggaggcga ttcagcattt aagttattta
     2941 taccgatact tgctggtttt atagctaaat caatagcagg aagacaagga tttgctcctg
     3001 ctatgatagc tggttttatg gcagttattg gaggttcagg ttttataggt ggtatggtag
     3061 ctggttttgc aacaggatat atagctttag gagtaatgaa gataacagaa aatttaccag
     3121 atgtattaaa aggagtaaat gctgtattga tatgtccact tttgattgca tttacgagtg
     3181 gagctataat gttctttgta gttaatacgc cagcatcatg gttaaatgaa gctttacaat
     3241 cttggttaca aagtatgtct ggaactaata aagtattatt aggtgcaata ttagcaggga
     3301 tgatggcatc agatatggga ggacctatta ataaaacagc ttcagctttt ggattggcta
     3361 tgttttcaag taatatattt gaaccatcag cagcattaat ggtaggagga atggtacctc
     3421 cattagcaat atcattagct atgttaatat tcaaaaataa gtttactaaa gaagaaagaa
     3481 agtcatctct accaactgct ataatggggg catctttcat aacagaaggc gcaataccgt
     3541 ttgcagctgc agacccatta agaatgattc cagcaaatat aattggttct tgtataggtg
     3601 gggcaatatg tatgagttta gatataactt taatggcacc acatggggga atatttgtaa
     3661 taccatttgc atgtagtgca ccagttgcat atatagcttg tatattgatt ggtacagtaa
     3721 taacagcatt gattataggg gtaacaaagc ctactataag gagtgaaaat agtgaagttg
     3781 aattacttgg tgcttaaatt ataaataata gtaaaaataa ttaatggaat aacaacaagt
     3841 atatagaata ataataagga tatttagaat cagttaacta cttctaaata tccttaattt
     3901 ttattttgaa attatattat cttattatat caatttagta aggtgtatca taaattcttt
     3961 tatgatacag aagtatctta gtaattttac atagttagta aatatgttaa aatagtactt
     4021 aatataaaca tagaaatata taaacatagg tagggagggc taagtatgct tgtgtcgagg
     4081 caaattgaat ttcttaaatt gatactaaac gaaaataaat atagacctat aaaatacttt
     4141 aaagataaat taaatgtatc tgataaaact ttacaaaaag atttgaagat gattgagaaa
     4201 tatttagaaa cttttaatat aaaaattgat ataaaaagag gctatggaat attaatagaa
     4261 gatttagcaa agaagaacat agacttaatt aatagtttaa atatacaatc gaaggaaaat
     4321 aaatctttat cagtagagca gagaagaatg gagattttaa agtatttatt gctaaattca
     4381 aataatataa cttcaataaa tcagttagca gataaatatt atgtaagtaa aacttctata
     4441 gtaaatgatt ttaaatatat agaggcatgg ataaaagaat acaatttaaa acttaataaa
     4501 actttagaag gaactaagat tataggtaaa gaagtggata taaggaaatc gattgccaaa
     4561 atgatggatg atttattaga tgaaaattat gaacaacatg atatatctga gttaacaaga
     4621 ttagattctg ctacatttag tgcattgata aatttatttg atattgattc tattatattt
     4681 gtagaaacta taattacaga attagaatac aatttaggat atactataaa tcagccctac
     4741 tatataaatt taattaccca tatacttata tgtttaaaga gaatcgaaga aggtaatcaa
     4801 atagaatctc agaaagaaag agaaatttct gtagataatt tggacgagtt agtatataaa
     4861 aatgtcacta cacttataga taaaatagaa aatagataca gagtaaaaat aacgaaagat
     4921 gaacttatgt acatatatat ttttttggta tcatctggat ttagtagcga aaataatagt
     4981 agttacaatg atgaatcaga tagattaagt gaaagtgaaa aagtttcatc tataatgata
     5041 gagaatatgt cacaattttt aaatataaat ctcaaaaatg atgacttact acaaaaaagt
     5101 ctagcatctc atgtaaggcc tatgttaaac agattaagat atgatatcca aataaaaaac
     5161 ccgttgttag gagagataca agagcgattt tcagatgtgc tgggattatg tatgatgact
     5221 ataaatatta tgacagagta tgataatctg aagaatatta gtatagatga agtttcatat
     5281 cttgccacat attttcaagc ggcaatagaa agaaatatga gtagtaaaag agtaatagta
     5341 gtttgtcata caggatatgg aacatctcag ctccttgcag ccagattaaa aagagaattt
     5401 cctgaatgga ctatagttga tatagttcct atgcatcagt tgagtaagcg aaatttagat
     5461 gatgtagact ttgtaatatc tactgtaaac ttagatttga aagataaatt acatatagtt
     5521 gtatcagtac tattaatgga aaatgatatt aataatataa aaaatgcttt actttctaaa
     5581 cctaaaaaaa gtatgaactt agatacaaac ttattaggaa tgtatttaga agataatata
     5641 tattttaatt ttgatgtagc tcagatggaa agtttaatag gtgtgaattt agggagtaaa
     5701 tatgaatcta tttcattagg aaaagattta aatatatata tacataagca aagtaaatta
     5761 aataaaggga taatgaatat caataatgtt actagaacta ttgatattta tttagaaatt
     5821 agtaatcaaa gttttttaaa gaatatttta atggaaatat caaatttata taggcaaaag
     5881 aactatataa actatataat agattgcaag agcaaagagg atattaaaat attatttaag
     5941 aataatctat tggagaaaac aaatggaaat tagagaaata ttaacagaag aaagcattat
     6001 tttagatttg aaatcaacta caaaagaagg cataatacag gaattagtag ataaattata
     6061 cgatacagga agtataactt taaaaagtaa gtttataaag gcattaaaac atagagaaaa
     6121 aataggctct actagtatag gttatcaaat ggccatgcca catggaaaaa caaaatatgt
     6181 aaagaaggca tctgtagtaa taggtaaatc taaaaaaggt gtagattggc caagtataga
     6241 tggcttacct gtaaaagtaa taattatgtt tgctatgcca caatctggag gcaatgaaga
     6301 tatgatatta atatctaaaa tagcttacat tttaggtggt aaaaatatag gtgaaagact
     6361 tttaaatgca catacaaaaa aagaaatatt agatatattt tatgaagtgt aggtattgta
     6421 taaattataa atttgaggtt tttaaaataa aatgtgtatt cttttaaata gtataaatta
     6481 atttttagac actagagagg agaggatata tatggcaata tatttagaaa tgcctaaatt
     6541 aatacatgaa tttccttttc gtactctatc aaatgaagga gaagtattaa caacaccaca
     6601 ctggcataag gaaatagaaa tattacttat aacagaaggt gtagtaaatc tatttataaa
     6661 tgacaagcct atgcaattaa aaaaaggcga gatagtgata ataaaaggag gagatattca
     6721 ttatatactg ccatcaccgg gaagtgaaag attagtattt caatttgata tatctttttt
     6781 taatgattta attttattaa atgaggagaa agaaagctta attaatttat tcagtagtat
     6841 aaaaaaatgt agtagagatt ggtcagcaaa aataagaaag tcagttttcg atatattgat
     6901 agatatctac aatgaagacc tatacaaaat agaaggatat agttatgcaa taaaaggtaa
     6961 aatgtataat ttaattccta tattatatag acagattcct agagaaattg aagaaaatac
     7021 agtagaatat gaaataaact ctaaagagat attagagaga ttgaacaata tattcaaata
     7081 tgtagaaaaa aattataaac aacctataaa attagaagat gtgtcttatg aagtaggatt
     7141 tagtgtatat tattttacta aatttttcaa aaaaaataca ggaaaaacat ttataacttt
     7201 tttaaatgaa tatagattag aaaaagcaaa gtggatatta cttaataaca atatatccat
     7261 tgtagatttg gttgaagaag ttggttttgg aagtacaaaa actttttaca gagtatttaa
     7321 agaaaaaatt ggagtctctc cattagaatt taaaaaaaga tataatagct tggaaaaaaa
     7381 agggagtgtc tcaaaatgaa cttagttcat gaggcacttt cttttatatg aaatcaaata
     7441 tattttcagt atttcaacaa tgaaaattcc attcatttat aaagtctaaa ttttagttct
     7501 caatacaacc tctatttcga aataaatcct tttatatata taaatataca gcaatatatg
     7561 agaataagat aacaataatg aggatataaa aatgtatttg ctatatatat aattatatac
     7621 atagatatct aatattgata tttaatatga aagtattgaa aatagacaat attaattata
     7681 gtgagtaaaa aatataattg gatttttaaa ttgaattatt taaaaggtat agtatgaaaa
     7741 aaatcttata actaaatatg agatagaaat tataaaactt tatattgtga aagaggtaga
     7801 tgcatgaaat tagggaaaga ttttttatgg ggaggggcaa tttcagccaa tcaaagtgaa
     7861 gggttttatt tagatggagg aaaaggttta tctcagtttg ataaactccc attaaatgat
     7921 aagagattaa aagatgtatg tttagatgag gataatattt taaatgaaaa taatcaatac
     7981 tatccaagtc atatagggat taaattttgt gaaaattaca aagaagatat agcaatgtta
     8041 gcagaaatag gttttaaatg ttttagattt tctatatctt ggtcaagaat attcccaaca
     8101 ggagaagaaa gtgaaccaaa tgaaaaggga ttacaacttt atgataatat aataaaagaa
     8161 ttaaaaaaat ataatataga gccattaata acgataagcc actttgatat accttattct
     8221 ctagttgaaa aatattctgg gtgggctgat agaagagtgg ttaatttata tgtaaaatat
     8281 gctgaaattt tgatgaaaag atataaagat gatgtcaaat actggattcc ttttaacgaa
     8341 atgaatatga taatacatat tccatatata ggtgctgggt taacttttat agctgatgaa
     8401 aatcaacttc agaaaaaata tcaagcagca caccatcaat tattagctaa tgcaaaaact
     8461 atagaaatag gaaggtatat aaataaagaa tttaagtttg ggtgtatgtt agctgctgga
     8521 aaaacatatc catacacatg taaaccagaa gatgtatttg caactttaga gcatgaaagg
     8581 cataatttgt ttttttctga tgttcaagtt aggggtgtgt atccaagctt tatgaattat
     8641 tatttaaaat caaatcaaat aaaattaata ataaaagagg aagatttatc aacacttaag
     8701 gataatactg tagattttgt atcttttagc tattattcta gtgcttgtag ttcagctaga
     8761 gctgaaggtt tagaaaaatc aaaggcaaac ggaccagaaa ccataaaaaa tccatatctt
     8821 ccaaaatcaa atagtgtatg gcaaaacgac cctgtagggt taagaataac tatgaatcag
     8881 ttatatgaaa ggtatcagtt acctttattt atagtagaaa atggactggg tactcaagat
     8941 attgtaaatg aagatggaaa aataattgat gattatcgta tagattacat gaaagaacat
     9001 attaaaaata tgattcttgg agtaaatgaa gatggaatag agcttatagg atatcttatg
     9061 tggggatgta tagatgtaac cagtgtaagt gaaggaaaga tgacaaaacg atatggcata
     9121 attcatgttg attctgatga ctatggaaga ggctcattta aaagaactaa aaaacaaagt
     9181 gctttttggt ataaaaaagt aatagaaaca gatggagaaa ttttatttaa tgatatggaa
     9241 actcatgcat aattcatttt aagaaaggag tgttattatg agtttaatat ttactactaa
     9301 ggaccaactt atagagcaag ggtttagtaa ggatacaata aaatccaatg aaacgctgtt
     9361 tacactagcg aatggacatt taggagttag agggaatatt gaagagtctg attttaataa
     9421 tgagtatgta gataatatgg gtacatatgt aaatggattt tatgaagaat ctccaattac
     9481 atatggagaa agcgcttatg gctatgcaaa acaaaatcag actatctgta agttacctaa
     9541 tgctcatata gtaaatttct ctattgaagg agattatttt gatttatcaa aaggtattac
     9601 aagtgaacat tctaggatac tagatttaaa tgaagggatt cttaaacgtt catttatatg
     9661 ggaaaactct tgtggtagaa aagttaaagt atctatagaa agattagtat cacaagatat
     9721 agaagaatta atgatgattt catatacaat aacaccattg aattttaatg gaaaaataaa
     9781 atttaaaaat aaaatggaaa atagtatgga gaagtttaat tgtgaaaaag tagatgaccc
     9841 aagagtggca tctataaata agaagcaatt taaaattaat gtaaaaaaat ataacaataa
     9901 gtattttatg aatttagtta cagaaagaag tagattaggg ttatggtgtg ggtttgattc
     9961 atatattact tgtggaaata ttacaaatga gacctataat gtttcagatg aatgtattga
    10021 gcgagaaata gaagtctatg ccaaaaaaga tgaaaggtta aatttagaaa ttgttgtagg
    10081 ctatagtaga gtatattctg atttaaatct aaatgaagaa cgtttaaaat cattagaatg
    10141 tctggagtcg ttaaaatgtt tagaatcaga agcacattta aaatcattag aattggtttt
    10201 aagagaagca tgtaatattg gatttgataa agtcaaaaat attcagagaa agaaaatgaa
    10261 tgagttttgg acaacctctg atgtagagat tgaaggcgat aaaactcttc aactggggat
    10321 aaaaaccaat ttatttcata tttatcaatc agcaggaaga gatggaaaga ctaatatatc
    10381 agcaaaaggt cttacaggtg atggctatga ggggcattat ttctgggata cagaaatgta
    10441 tatattacca ttttttatat atactcaacc atccattgct agagctttat tagattatag
    10501 atactcaatc ttagatgcat caagagaaag agctagagaa cttggaataa ataaaggttg
    10561 cttatattca tggagaacta taaatggtaa tgaatgcagt gcatattatc cagcaggaac
    10621 agcacagttt catataaatg cagatatagc ttatggtatt agaacgtatt ttgaagcaac
    10681 taatgatgaa gaatttatga tgcaaaatgg cttagaaatt cttatagaaa cagcaagatt
    10741 ttgggttgaa tttggagatt atatagaatc aaaaaataat aaattctgta taaatggagt
    10801 aacaggacca gatgaatata cagcaatagt aaacaataat tactacacta atttaatggc
    10861 taagtataat ttaatatatg cagtacagat ggtagataaa tttaagcata atttattttc
    10921 aaatgaagta tttagaaaag ttaaattaca agaatatgaa gtgaaaaact ggataaatgc
    10981 cgctcaaaat atgtatttac catatgatga agagaagaaa ataagcaaac aagacgattc
    11041 atttttagaa aaatcagtat gggattttaa aaatacacca aaagaaaaat atccattact
    11101 tcttcattat catccattaa caatatatag atatcaagta aataaacaag cagatactgt
    11161 actttcagcg ttgcttttcc ctgatgagtt cagcttagaa caaaagaaga gagattttga
    11221 atactatgaa aaaataacta ctcatgattc atctttgtca aggtcaatat ttggaatgat
    11281 ggcaagtgaa attggaaatc atgaaaaagc ttataactat tttatggaca cagcattaat
    11341 ggatattaat aacctacaaa aaaatacaag tgatggaata catgctgcta atatgggtgg
    11401 aacatggatg agtttagtat atggatttgc aggaatgaaa ataaaaaatg ataaattaag
    11461 ttttgaacca aggataccta aacactggaa aaatattaaa tttaatatat tatttagaga
    11521 taatttagtt tctgtaaata tagaagaata taaatcttat tataagttaa tcaaaggtag
    11581 aaatataacc atattacatg ataataaaga aataaaatta tcaaagaaca taatagaagt
    11641 taataattgt tttaaaggga gtaattaata tgaaagcagt cttatttgat cttgatggag
    11701 ttataacaga tactgctaag tatcattttg aagcttggca tgagatagca aaatcgctag
    11761 atatagaaat agatgaaact tttaatgaaa aattaaaagg cataagcagg gaagaatcat
    11821 tagataaaat attgttatct gctaataaag tagatagtta tactaaagag gaaaagttaa
    11881 aggtgtgtga agaaaaaaat aacatttata taaagttaat aaactcatta acatataaag
    11941 atatattacc agggataaat gagcttataa aagaacttaa aaaaaataat gtgaaattgg
    12001 gaatagcatc tgctagtaag aatgcaccta ggatattaga atctttggga ataaaagatg
    12061 aatttgattg tatagtaaat ccatctttat taaaaagggg aaaaccatat cctgatattt
    12121 ttattgaagg gagcaagatt ctaaatttaa aacaatatga ttgtgtagga atagaagatt
    12181 cttattcagg tattaaagct ataaatgctg ctaatatgac atcaattggt gtggggaaaa
    12241 aagaagtact aggaaaatat gaccatattg tagaagatac tagcttatta aattacaata
    12301 cactattaca agcttggagt aaaaagatta atttggatta aaattatatt aaaaattatt
    12361 ttaaaagaga ggataaggta tgaagaataa atatgaagaa gtagctcaat tgataattga
    12421 tggacttgga ggaaaggata atataattga cttaactcat tgtatgacac gattaaggtt
    12481 catcttaaaa gatgaaaaga aagtcaatgc agataaatta aaatctatag ataaggtagt
    12541 tggagtaaac tcaacttcaa ctcaatatca ggtcataata ggtaatgagg taggtgttgt
    12601 atttaatgca ataataaata aggggataaa taccacagga gatattaaaa gcgatataaa
    12661 aaataaaaat aaaaaaaata gtattttaaa taatataata gatacaataa ttggttgtat
    12721 gacacctatg ataccagccc ttacagcatc tgggatgata aaagttatat tatctctttg
    12781 caccactttt aattggctat catcaaattc aagtacatat agagtattag atataatagg
    12841 agatgtagca ttttacttta tgcctataat acttgcaata aatgcagcta aaaaatttaa
    12901 tgttaataca tcaatagcag ttatagtagt aggtgtgttt ttacatccaa atttctctgc
    12961 atgggtatca agtggggacc ctatatcatt tataggagta ccaattcaag gtgttatata
    13021 tgcagcatct gttatccctg cacttttaac agtatggatg atgtcatata tagaaaaatt
    13081 cattgataaa ttaacaccta gcatgttaaa aacaatttta aatccaactt tagtattatt
    13141 aataagtgcg ccaatagcat taatagtaat tggaccaata ggaaatttct taggagaggg
    13201 attagctagt ataataaatt tactacaagg aaggttagga tttataatgg tttgtttatt
    13261 agcagctgca atgccattta tcgttatgac agggatgcat catgcattga ctcctatatt
    13321 catatcagca tttgcaacta caggacaaga atcgctaata ttaatatctc aagtttgtgc
    13381 taatttagcg caaggtggcg cgacattagc tgtagcatta agaagcaaag atagtaagat
    13441 gaaacagtta gcatcagctg catggatatc agctattatg ggtataactg aaccagctct
    13501 ttatggagtt acattgaaac ttaaaaaacc tgctatgtta gcatctatag gagcaggaat
    13561 cgcagggtta tttgcaggaa ttactcatgt gactttatat gttccacaaa atagtataat
    13621 ggcagtactt ggattatcag gggaaaaggg gatttcaaat atagtaaatg gacttatagt
    13681 tatatttata gcattggttg ctccatttat tatgacatat gtttttggag ttgaaaaaaa
    13741 tgttaaggaa ggtataggta aattaaggaa gtgtgttaaa tagtatgaga taaaaaaatt
    13801 catgataaat atttataata aaagtgatta acctataaaa cttagtaaat aatagttata
    13861 taataattaa gataaactca atttaaaact ttaaaaaata cgattcttaa atagtcaaac
    13921 tttagtaaaa tatataaaaa tatgttttgt taaggtttgg catatttttt attttggaga
    13981 agaaatttga tacctatatt cctactacag gaagatggtc tcttgggtgt actataggat
    14041 ttataatgta taaaattgta catgtaaaag ttattagtcc attaggatgg tgattaataa
    14101 ataatatgat taatagaata gtttagcctt gtattgtaat gtttattaaa gaatcatttt
    14161 atgccataag ttataagcat tttttatgtg gagagttcta taaaatgtaa taaaaaagta
    14221 gttttataat agtatctgta attcttaa
//
LOCUS       DAKBNN010000095        14196 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000095 DAKBNN010000000
VERSION     DAKBNN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.095"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(390..1028)
                     /locus_tag="OUB02_002322"
     CDS             complement(390..1028)
                     /locus_tag="OUB02_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HCT8886079.1"
                     /translation="MDHFALIFLGITLIGIILAFINVRIPPISNYPDTYVDGSTTSKI
                     VSSTQGTRLQRAIIQSSMRANQVTLKNFTSKIDNMSFYVLWLTPIIIFWGALALIVSL
                     YTPLLTWVSYPIELVLKLANISEPAITASAIMSGFADNYLPVILGKGLESIESKTIIT
                     MMSILQLIFMSEIATLLQSCKVVKSFKDIIIIFLQRTIIALPFVIIFTKLIL"
     gene            complement(1041..1721)
                     /locus_tag="OUB02_002323"
     CDS             complement(1041..1721)
                     /locus_tag="OUB02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HCT8886080.1"
                     /translation="MNISQTKGFFKFLILTLLGIVFLLIPFRFENGMDTILFHYLKLF
                     ISENHAVIQTIVVLLVCLSATLSLIDQLLPNTILRKQRLLKKLFSTTPFYVFNRIMGV
                     IIAMLCYLQIGPKFIISIDTGGAMLDLGTQLSVLVPLMLLFQTLILEFGAMEFLGQLI
                     GFIAKPLFKLSEICAVNIISAWVGPGNAAIIGTKELFEKGYFTVKESAVIGSQFSISS
                     IGWVVLVC"
     gene            complement(1740..2657)
                     /locus_tag="OUB02_002324"
     CDS             complement(1740..2657)
                     /locus_tag="OUB02_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCT8886081.1"
                     /translation="MKVSIIGAGAVGATTAFTLAKTSFVDEIAIVDIDQNRAKGNALD
                     ILHGLSLMHETRIVSGDYDCAINSDVIVITVGVPEKVGESRLVPLQKNVKILQDIIPK
                     ITSTSPNGLLLVVSNPVDIISYFSQKISGWEAKRVIGLGTTLDSARLNYLIARDLKIS
                     QTDIQGLVIGEHGDSQVVAWSQTSVKGTPFLDYVKSNNISLQEDYCNNLAQEVKDTAF
                     DVWDMKGPNCYCVALAIERVIKAIARNEHAILPVSQPADSEMYISQPHIIGRDGVQRR
                     VILSYDTQELNAFDTSYQSLQDIVKQIEI"
     gene            2907..3659
                     /locus_tag="OUB02_002325"
     CDS             2907..3659
                     /locus_tag="OUB02_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCT8886082.1"
                     /translation="MEKKSPIIKNIVDSFRIIDVINEKGSIGIAKISEILSIPKTSVF
                     RIVKTLEEIHVIKQLENSDYTLDYRLLSYAKGTSQENKLISVAEPYLQKISKETGESV
                     NLGIRYQNEVIIVKSIEGEFYQLQTTLIPVSPLYCSGMGKLFLSQESEEFLQDYFNNL
                     EKRTVNTITEYNEFLLAKKRILELNLSIDNEEYEYGLSCYAVPIFDCNNDLICAISIT
                     GPSNRLMHKGEEYLIKVLKEQANNLQTEFSKL"
     gene            complement(4041..4396)
                     /locus_tag="OUB02_002326"
                     /pseudo
     CDS             complement(4041..4396)
                     /locus_tag="OUB02_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4561..5718)
                     /locus_tag="OUB02_002327"
     CDS             complement(4561..5718)
                     /locus_tag="OUB02_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCT8886083.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKIVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            5872..6441
                     /locus_tag="OUB02_002328"
     CDS             5872..6441
                     /locus_tag="OUB02_002328"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCT8886084.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(6623..7039)
                     /locus_tag="OUB02_002329"
     CDS             complement(6623..7039)
                     /locus_tag="OUB02_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886085.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(7265..8359)
                     /locus_tag="OUB02_002330"
     CDS             complement(7265..8359)
                     /locus_tag="OUB02_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886086.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(8782..9723)
                     /locus_tag="OUB02_002331"
     CDS             complement(8782..9723)
                     /locus_tag="OUB02_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HCT8886087.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            complement(9716..10543)
                     /locus_tag="OUB02_002332"
     CDS             complement(9716..10543)
                     /locus_tag="OUB02_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCT8886088.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(10714..11097)
                     /locus_tag="OUB02_002333"
     CDS             complement(10714..11097)
                     /locus_tag="OUB02_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCT8886089.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(11066..11353)
                     /locus_tag="OUB02_002334"
     CDS             complement(11066..11353)
                     /locus_tag="OUB02_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCT8886090.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(11386..12543)
                     /gene="alr"
                     /locus_tag="OUB02_002335"
     CDS             complement(11386..12543)
                     /gene="alr"
                     /locus_tag="OUB02_002335"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCT8886091.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(12558..13148)
                     /locus_tag="OUB02_002336"
     CDS             complement(12558..13148)
                     /locus_tag="OUB02_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886092.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(13169..13630)
                     /locus_tag="OUB02_002337"
     CDS             complement(13169..13630)
                     /locus_tag="OUB02_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCT8886093.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(13645..14025)
                     /locus_tag="OUB02_002338"
     CDS             complement(13645..14025)
                     /locus_tag="OUB02_002338"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCT8886094.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         4752 a         2514 c         1623 g         5307 t
ORIGIN      
        1 ccataaataa tataaaatag ggcttgtaat attagaatta ccaaccctat atcttctact
       61 tatgtatatt tttaatataa ttcacatatt ttatctttat aaatttaatt tttaacaaat
      121 tcaattagat atccaggttg gctatataat gctttacata atgatagttt gaaaatcaca
      181 aaaataaata gaatattcaa tataactatt tgaagtgatg acttcaaaat ttctttaaat
      241 aagaaattaa ataaatatta aaaaattaac aatatatttt agcaaactcc cttatacaaa
      301 agatatttca aggaatgttc atcaatctaa attatgaaaa gaaagactat taaagaataa
      361 tcattcaagc actttaaaac ctttaaatct tatagaataa gctttgtaaa tataattaca
      421 aatggtaggg cgataatagt tctttgtaaa aatataataa taatatcttt aaaacttttt
      481 acaactttac atgactgaag taatgttgcg atttctgaca taaagataag ctgtaggatg
      541 ctcatcatag taatgattgt tttgctttca atggattcta atcctttgcc caagataact
      601 ggtaaatagt tatccgcaaa tcctgacata attgcgctag cagtaattgc tggttcagag
      661 atatttgcta acttcaatac taattctatt ggataagata cccaagttaa tagaggagta
      721 tacaatgaaa caataagtgc taatgccccc cagaaaataa taataggtgt tagccataaa
      781 acatagaagc tcatattatc aatcttactt gtaaaatttt ttaaagtaac ttggtttgca
      841 cgcatacttg attgtataat agcacgttgc agtcgtgtcc cttgtgtgct agagacaatt
      901 ttagaagttg ttgaaccatc aacataagta tcaggataat tagaaatagg tgggattctt
      961 acatttatga aagctaagat tatacctatc agagttattc ctaagaatat taaagcaaaa
     1021 tgatccattg tgtctaaaac ttaacaaacc agtacaaccc aaccaatact tgagatggaa
     1081 aactgacttc cgataacagc ggattccttt actgtaaagt atcccttttc aaaaagttct
     1141 ttagtaccaa taattgcagc attaccagga ccaacccagg cactgattat attcacagca
     1201 caaatttctg aaagtttaaa taatggtttt gcaataaacc ctatcaattg tcctaaaaac
     1261 tccatagcac caaactctaa aataagtgtt tgaaatagta gcatcaatgg tacaagtaca
     1321 gaaagttgtg ttcctaaatc aagcattgcg ccaccagtat cgattgaaat gatgaattta
     1381 ggacctattt gtaaataaca aagcatagca ataattacac ccatgattct attaaataca
     1441 tagaaaggtg tcgttgagaa cagtttctta agtaatcttt gtttacgtaa aatcgtattt
     1501 ggtaacaatt gatctataag agataaggtt gcactaagac aaaccaacaa gactacaatt
     1561 gtttgaataa cagcatgatt ttctgaaata aataatttta gatagtgaaa taatattgta
     1621 tccatgccat tttcgaaccg aaatggaatc aataaaaata caatacctag taaagttaag
     1681 ataaggaact taaagaaacc ctttgtttga ctaatattca tgaaatcctt ctcccttaat
     1741 taaatttcaa tttgtttaac aatatcttgc aatgattgat aagacgtatc aaatgcattt
     1801 aactcttgag tatcataaga taagataact cttctttgta caccatcacg tccaattata
     1861 tgaggctggc tgatatacat ttctgaatca gctggctgac taactggtaa aattgcatgc
     1921 tcattacgag cgattgcttt aattacacgc tcaattgcca aggcaacaca gtaacaattt
     1981 ggtcctttca tatcccatac atcaaacgca gtatctttaa cttcttgagc caaattatta
     2041 cagtaatctt cttgtaacga gatgttatta gatttaacat aatccaagaa aggtgttcct
     2101 ttaacacttg tttgactcca agcaacaact tgagagtcac catgttcacc aattactaaa
     2161 ccttgaatat cagtttgtga aatttttaaa tcacgagcga ttaaataatt taaacgagcg
     2221 gaatcaaggg ttgtacctaa accaataaca cgttttgctt cccaccctga aattttttga
     2281 gaaaagtaac ttatgatgtc gactgggtta cttacaacca ataataatcc gttaggactc
     2341 gtacttgtga tctttggtat aatatcctga agtatcttta catttttttg taatggaacc
     2401 aaacgtgatt cgccaacttt ttcaggtact cctacagtaa ttacaataac atcagagtta
     2461 attgcacaat cataatcacc tgaaacaatt cttgtttcat gcattaaaga taatccatga
     2521 agaatatcta atgcatttcc ttttgcacgg ttttgatcaa tatcaacaat tgcaatttca
     2581 tctacaaaac ttgttttagc aagtgtaaaa gcagttgtag cacctacagc acctgcacca
     2641 ataatactaa ctttcatttt attaccccct tttataattt cggaacacgt tccaaaatta
     2701 taatactatt ataaaatcat atagtcaata cctttaataa taaataacaa agtttttttg
     2761 tttttatctc ttttttataa agaaacaact aaaattagtg aaaaaatact aataattagt
     2821 gagctttaca tatatgaagt ttgaaaatac gtcttgccat tttaactaat tagacttaga
     2881 atttaaaaga aaggtggtgg gcaattatgg agaagaagag tcctattatc aagaacatag
     2941 ttgatagttt tagaataatt gatgttatta atgaaaaagg aagtataggt attgctaaaa
     3001 tttcagaaat attatctatt cctaagacta gtgtctttcg tatagttaaa acacttgaag
     3061 agattcatgt tataaagcaa ttagaaaaca gcgactatac tcttgattat cgtttattat
     3121 cttatgcgaa gggaacaagc caagaaaata aattaatttc tgtagcagaa ccctatttgc
     3181 aaaaaatcag caaagaaact ggagaatccg taaatctagg tatccgttat caaaatgaag
     3241 ttattattgt aaaatcaata gaaggagagt tttatcaatt acaaacaacc cttatacctg
     3301 ttagtccttt gtattgttca ggaatgggga aattattcct aagtcaagaa tccgaagaat
     3361 ttcttcaaga ttactttaat aacttagaaa agcgtactgt aaatacaatc acagagtata
     3421 acgagtttct gttagctaaa aaaagaatac tggaactaaa tttatcaata gacaatgaag
     3481 agtatgaata tggattgtct tgttatgcag ttcctatttt tgattgcaat aatgatttaa
     3541 tatgtgctat tagtattact ggtcctagca atcgattaat gcacaaagga gaagaatatt
     3601 taattaaagt ccttaaagaa caagccaata acttgcaaac tgaatttagt aaattataga
     3661 aagtacttta gactaatgtt aagaataata caacaatata atatacgata aagtaagaaa
     3721 aggattcggt gaaataactt cctatacaac tatggtggta cccatgatat agatattgcg
     3781 tgactctaaa taaattaaag caaaaacctg ctgtatacat gttaatagaa tctacggagt
     3841 tttcactaca aataaaaatt tattctttaa gtaataatta aacatcaaag taaggctatg
     3901 cctttaaaaa tcatagcctt actttgatgt ttttttattt ttgcaaaaat tattactcct
     3961 ttaagaaaat ttcttaattt ttttgaaata tttctttatt aacttagatt ttaaattttt
     4021 tcttcaaaat aaatacttaa atgagctaaa ggtataactc tatcagtaat tttatctatt
     4081 agagaatgag atatatcatt ttcataattt ctaattacct ttaattcaaa atctccattt
     4141 cggtctcttg gaatatttaa agttatttct ccaaaatctg atttaatatt tttatttcta
     4201 tatccctttc tagagttttt agtattttta ttcttattgt catatctatc ataacctaaa
     4261 tggtcatcaa gctatacttc taacatttga tgaattgtac ttgcaaataa atcttttcgc
     4321 atatcttgaa tatctgtagt tgttttaaca tcatattctt caataagttt tttaatattt
     4381 tgtttttatt aagcataaaa agtgtgcacc ttcctttata tagtgatatt taatatttca
     4441 attatcacta ctttttagaa gaatacacac tttattttaa agaccctaaa aagttaattt
     4501 tgagacactc cttttattaa ggttctttat tttacaatat aaatttataa aaattaaatt
     4561 ttatttatca atctgttctc taagccactt agttgctaca tctaactgtt tattttcatc
     4621 tttctcttca atagtaggct ttataccttt ttcattgata taatccccat taggtgtaaa
     4681 atattgagca gtagttagct tgtatcctgt tccatccttc aatcttacaa ctgattgtac
     4741 caacccttta ccaaaagttg tagtccctac agaaatacct tctttattat caacgattgc
     4801 agctgttaat atttcagcag cagaagcact ttctccatta gttaacacta ctattggaag
     4861 tcccaatttt tctttatcag atttcaagta ctcagtattt cccttattat cttttgtata
     4921 tactatagtt ccttcaccaa ttaactcatc agcaacttct ttacatatat ctagcattcc
     4981 accaggatta tttctaaggt ctattactaa tcccttgata ttttttgctt ttaattcttt
     5041 taaagcttta tcaaagtcag tatatgtact tgatataaat tgggtaattt ctatatatcc
     5101 taaatcatta tcaattactt tactttctat actcttctca attatctggt ctgtttttac
     5161 tttaaagttc aattgtttat cttctcttaa tatagtcagt tcaactattt tccctttttt
     5221 acccttcatc atacgaacag cttcatctga atttttatat gagacagatt ttccattgac
     5281 ttttactaat ttatcccctg attttacacc tgctttctct gctggagaat ctttcatggg
     5341 aactactact aagccaccat cttttgatgc ccctatatac atacctacac caacataaga
     5401 accttcacta tcattaatta gattctccat ttcatcttta gtataataac cagaatactt
     5461 atcatttgtt cctagaaata gaccttttga tgctccattt accaaatcag tatctgatac
     5521 ttttttataa aaatcttgtt ttatgataga ttctagtcct aacaacttat catatttttt
     5581 atagtcttca tagagttctt tcgatattac aactttattc cccaatgtca actgaaatgc
     5641 tgatgtaacc atagctgtta taattactaa aatcacccct aaaaaaatag ctttcttctt
     5701 tgaaatcaca agatacatct ccttttacat aaactattat tattatatga ctagctgtta
     5761 tttttattac atttacccaa atatattgct ttattacaat aaattatact aaaatttata
     5821 ttaaatacaa ttgctgtaca cattataaca tattaaggga ggaattattt tatgaaaaaa
     5881 gaatttagaa aaaaagttat tgaatcaaga aaaaataaga gtgcaaactt tgtacttagt
     5941 aattcaaatt taataactga aaaattactt caaatggaag atataagaaa tgcaactaat
     6001 ataatgcttt atctaaattt taacaatgaa gttcaaacag ataatttaat caaaaaactt
     6061 ttgtctctaa agaaaatagt atcaagtccc atcacaataa aagaaaatca tacattaatc
     6121 cctactcaaa taacagattt aaaagaggga ttgagaattg gagcatatgg aataagagaa
     6181 cctaacgaaa aatcacctac aattgatatt aaggccttag atgttgtaat tgtaccagct
     6241 gtagcatatg atatacattg ctatagatta ggatatggtg gaggatttta tgatagattt
     6301 ttagaaaact taagaaaaga tgctataact atcggcatag catttgactt ccagatattc
     6361 gattctattc caaaagaaac tcatgatgca caattagact acatagttac tgaaactaga
     6421 attatcactc caaatagtta gaatattaag tatttccaga ccccatttaa tgtagtaata
     6481 aatactactg ttaatatctt actttataag cattttaagt catggctttt acaacaataa
     6541 gaaccataca caatttactt acattaataa aaaccatgac tttattgtac aatatgcagt
     6601 tatttaatta taaaatacct tcttatttcg tcgcatttaa ttctattcta tctggaagtt
     6661 cactcatatt tgtttcaaca actatgtatg gaaaagaatc ttccttatct ttatacttta
     6721 tcttgactct caaaacatcc tttccttcct caaccacact ttctattttg tccatttcta
     6781 taccatattt attatttcct ctagtcacta taatgtatat tttgttatcg attttagccg
     6841 ctaatgctct ttcttcattt gtatacttat ccatcacatc taatatcttt tctggtattg
     6901 aatttctttg taatactgta taatctatgg gtttagattt ctctacaaac atacttggta
     6961 tgaatataaa tccccctata acgaatacaa gagctataac tccataaatt atacctttaa
     7021 tattaaactt tacattcaat cttatcactc ctcccatata ttctaaatat atgatttata
     7081 aacttatctt agactctaaa ttattgatta gtatatattt atacataatt ataaatatat
     7141 gtaatgtaac taaaaaacat gttttttcta tttcataata aacgagctaa aaaataaaaa
     7201 gagctatatg aaaacatttt cattgttttc atatagctct tgagtttaca atattaaaaa
     7261 atacttattt gtcactgatt ccttcttgat atccagatgt ttttgtaatc tgtctataaa
     7321 gaacacaaac cagtattatg acgcccatat atccagtata tggatataaa ataccaacca
     7381 gcttatcaaa tggtaggaag ccacctataa gacctaatat agctgctatt aaagttatta
     7441 atttaaactt aggatgttta tcatctacaa atctattagt tactgaccat aataaaggta
     7501 cagctgttgt atatatacct aatataagaa ttattgagaa taaaactcca attactggtg
     7561 aaatcttatc tgctaagaat aatgatggta ttgcttttga atataagttt cctatatcag
     7621 aaagaatagc taagtgcatc attatagctg cagccataag tgctactcca cctaatatac
     7681 caccacaaat agcttctttt ttgttagcag cacttgctcc taatccagtt aagaatgcca
     7741 taacaacaat tgtgttataa gtagtatata ttacaccaga tagataggca tttggaactg
     7801 gtttagctat ctttacagtg ctaagaactt gtgcagcttc tcctatatta cctatgtttt
     7861 tagctaaact tataccacca actaaaagtg taaagattat tattataggc cctattccac
     7921 ctagtatctt tgataattta tcaagaccta gtgaaacagt taataatgct actactgcca
     7981 tacctactct acctacaaat ggatttaatc catagtattc agttaaagtt gctcctgctc
     8041 ctgaaatcat tactacgaaa acaccaaata agaataaagg tccaaaccat tcaaagaata
     8101 ttcctaaata cttaccacaa taaacacgat aaattttgat aggttcttta agttgaagct
     8161 ctttaccttt tgacataact gaagcaccca tccatgagaa taataccatt gaaattatcc
     8221 caccaataat acctgcaaaa ccataaaaag agaagaattg cattatttcc tgtcctgttg
     8281 caaatccaga accgattact gttgctacat acgcacctgc gaaactgatg actgttttaa
     8341 catttacttt ttctgacata tctcagttac ctccccatgt gaaatttttt cttctgatta
     8401 aacaattcga taaactacct acaatttcct ttaacataaa atacatgata actgttttat
     8461 agctttttag tataataact taaaacttat aatctgattt tataccctac tcctcctctc
     8521 ataatttttt tagattcttt tatctaagta gtcattatat ataaaattag tttctctata
     8581 aattatatat tagtcatatt tttctgaata gtcaatatat atttgaatat tttctctatt
     8641 tttgtaaaaa tttgaatatt ttattacatt tttgatatat tgatatcata taaaaaaaag
     8701 agagatataa attaatataa cactttatac ctctctttta aatatattat attattaaca
     8761 aacatgaata atttatatct cttacattct ttttgcaata atagcttctt taacagtctt
     8821 tactatgtca tcacaagtta attcatattt ttttaacaat tcatttggag taccagattc
     8881 tccaaatgta tctttaattc caactttctt tacaatggtt ggacaagact ctcctactac
     8941 ctcacttaca gcagaaccta atcccccaat tatgctatgt tcttctacag ttacaatagc
     9001 attcgtttct tttgctgatt ctaatattaa atctttgtct ataggtttta tcgaggacat
     9061 gtttataact ctagcactta taccttcttc ttgcaacttt tcttgtgcta atagagcttc
     9121 atttaccata attccacaag ctataattga tacatccttt ccttctctta agactgtacc
     9181 ttttcctatt tcaaatttat aatcttcatc aaataaaact ggtactgaac ttctccctaa
     9241 tctaacataa acaggtccat tatacttagc tatttcaaaa ataatttttt ctgtttcaac
     9301 tccatcagct ggaacaagaa ctgtcatatt tggtatagct ctcattatag caatatcttc
     9361 cacactttca tgagatgctc catcctctcc aacagtaaga cctgcatgag tagcacatat
     9421 ttttacattt agctttggat aacaaactga atttcttata atctcaaaag cccttcccgt
     9481 agcaaacata gcaaaagtac ttgcaaaagg tattttacca gcagttgata atccacaagc
     9541 agcgcctatt aaattttgtt cagctatacc catattaaaa aatctatctg gaaatgactt
     9601 gtaaaaatca tgtgtttttg tagactttga taaatctgca tctaatacta caacattgtc
     9661 atttatttga cctaatttta ctaatgcttt accatatgct tctctcgtcg ccattttact
     9721 cattgtcagc acctcctaat tcaagcaagg ctttttctag ctcctcatca cttggagctg
     9781 tcccatgcca tcctgcttga ttttccataa aagatattcc tttgcctttt atagtttttg
     9841 caactatcat tgtaggtttt ttaactgttg atttagctat atcaagagcc gcaaatattt
     9901 ggtcaaaatc atgtccatct atctcaatta cattccaacc aaaagcttta aatttatcaa
     9961 ctattgaacc taaactcatt acgctttcta tatctccatc tatttgaagt ccattattat
    10021 ctaaaaaagc aattaaatta tcaagtttat aatgtgcagc actcatagca gcttcccaaa
    10081 caattccttc ttgaacctca ccatcaccaa gcaaagtata cacattccac ggagcattat
    10141 ccaactttga tgccatagcc ataccacatg caactgaaaa accttgacct aaagaacccg
    10201 tagatatttc cacacctggc gtgcctttca tgtctggatg accttgtaac atccttccta
    10261 tcttccttaa tccattcagt tcttccttat caaaatagcc tttttcagcc aatgtagcat
    10321 ataatgctgg tgcagcatgt ccttttgata atacaaatct atctctatcc tccattttag
    10381 gatttgaaga atccacattc atttcatcaa agtataacgc agtaagtatt tcaactactg
    10441 ataatgaccc tcctggatgt ccagatttgg ctctatgtat cattgaaact atatctcttc
    10501 ttatgatacg cgcaatctca tttaatcctt tatggtctct catgcaatta cctcccttga
    10561 gttcgtaatt ttatatcaat tccatattat tatatcactt ttatgctatt tttttgcgtt
    10621 atttttaatt ttattaacat agtacattaa aattaaacct taagagatat ttaaaagaaa
    10681 aaagggattg tctaataaaa gacaaatccc tctttaaaaa gtaaacaatc ctaaacttat
    10741 ttgaagtcca tcatctactt ttatcatcat attttcatct agacaaccaa ttttttccct
    10801 cagtctcttt ttatcgatag ttctaatttg ctctaaaaga attactgagt ctttattaag
    10861 cccatattca ttggaactta tctctacatg agtaggtaat ttggctttat taatctgaga
    10921 tgttatagct gatactataa ctgtaggact atatttatta cctacatcat tttgtattat
    10981 cagaacaggt ctaacgcctc cctgctctga accaacaact ggacttaaat cagcgtaata
    11041 taaatcacca cgctttattt cacaattagt actcaccttg ggcaatcttc ccttcatttt
    11101 tatcatcaga agatacttct tcaactgtca tacctaattc agctagtgac agattaattt
    11161 gacccatttc tctataaccc gttttcatag attctttaat atgaattttc tttttctgag
    11221 ttatatagaa ctgaaaagca gcttttgcaa aatcttctct gttactgttc tccatctgaa
    11281 ctatactatc aacttcagaa atcagagagt caggtaaagt aacttctatt ttttctttac
    11341 tcttatcgta cacaaaatcg cctcccagat tataatatca tggttttatt ttagcaaata
    11401 actgtttatt tgtacaagct cattattttc catataaaca cggtcaactc ttcttgatat
    11461 catacataac acttcatagt ttatagttcc taagtcttta gctatacgct cagctgtaac
    11521 ttcgccttct ccaaatagta taacctcatc tccaactttt atgtctatat ctttgtcaat
    11581 tctaaccatt atttgatcca tacatattct accaactaca tcaaacactt ctcccttaat
    11641 aagaaccttt ggattttttt ggattctagt aaatccatct gcgtatccta ttggaactgt
    11701 agctattgtt tctttacctg tagttgtgta ttttagtcca taacttattc ctacacctgg
    11761 ttcaacttgt tttatatgtc cgatttttga ttttaatttc atggctggtc ttaattctaa
    11821 tctatcttta aatacatcat cagatggata atgaccatat aatattattc ctgctcttac
    11881 catatttaat cttaaatcag ggcaatccat aatagctgca ctgtttgata catgttttat
    11941 agctattttt acaccagcct catctaattt atcagacata aatttataat tattagcttg
    12001 tttataagtg tactctttac ttacttcatc agctgtagca aaatgagtaa acataccttc
    12061 taaatctata tattctaatt tatttaattc tattatttcc tgtactgact cctcattagg
    12121 ttggaaacct attctagtca tccctgagtc tattttaaca tgaacacagg ctttttctcc
    12181 taaagatttt gcaatttcat ttatcttttg tgctgtttct aaagaataaa ctgtcatagt
    12241 tattttattt tttatagaat cttcaaaagc ttcgtctgga gtatatccca agttcaaaat
    12301 aggaagtgtt atgccatttt gtctaagttc aattccttca gcagttcttg ctactgctaa
    12361 gtaatctact ttttcttttt ctagcaattt tgcaacttct actgcaccat gtccatatgc
    12421 atcagctttt attactccac aaatcttaat atcttcttct aataaatttt taatattatt
    12481 taagttaaat cttaagttat ctagatttat ctctgcccat gtaggcactg ttattttttg
    12541 catgtcttta gtccccctta gtttctgtat tcaaaatctt tgtattttac tgtaaatctt
    12601 ggcactccct ctttatctaa cacttccatt ttttcaggat tttttgtgtc agcatttaca
    12661 tataatactt gcttgttaaa atatttatta tccccaggaa taaaagtctc taaaaccaaa
    12721 tgtcctttcg ataactttac tttcatctcc tcattttgaa gatagttttt tataaagtct
    12781 cctacaaaaa gatattgatt attttttcct gtattaggta actccactac atcactaatc
    12841 tttggatttt taactaatat tttatcacct tgatattcta tacttttccc ttttaaatgt
    12901 tttggggaaa ttacttctaa tttatagtta tcggtttttt tataagtatg aattaaaaca
    12961 taattgtgtg gacttttatt tccaactact tctacttcag ctttacaact gtaataattc
    13021 atatcagaaa tctgcttttg aaaatctttg tatacttctt cttttgtgga ctgtcgtttt
    13081 tggcatccta ttacaattat aaccagtgcc aagaacataa tacatactat ggtccacttt
    13141 tttctcacat cacacctcct acttcagttt attcattgta taaagctttt attatatcat
    13201 atctacaaat tataccttta agtttgttgt catcatctac tacaggaact ctgtttattg
    13261 attttttaat cattacatta gctacatcat caaacttatc atcttcatga acctttatta
    13321 tatctttaga cataagttct ccaaccttat aagcagctac ttgttttata tctttttcaa
    13381 ctttttttaa gtcatctaag aaaatcaatc cttgtaatag atttatatag agaggtgctt
    13441 ctatatattt ttctttttta agaatatctg tttcagaaat tattccaaca actctattct
    13501 ctgaatctac aactggcaga cctcctattt tttctgcaat tagcatatta gctacatctg
    13561 ctatactatc atcttgtttt gctactatta catctgttgt cattatttct tttgctgttt
    13621 tatccatcat aatgtaatca ctccttagtc cttaattatg gttatcgcat ttgctatagc
    13681 ataatctttt gaatgtgata ttgaaacttt tatttctaaa acattataat ctatgcacat
    13741 ttttgctaga ttattataag tttttactat tggttttccc atttcatttc tgagaacttc
    13801 tatatcttta aaattaaaca ctctaattcc tgtacctata gatttactta tagcttcctt
    13861 ggctgcgaaa ttacctgcta tactttctgc tttaaaattc ttagaattaa aatactctat
    13921 ttctctatct gtaaatatct tctccaaaaa cctattattt ttgtctacag cctttcttat
    13981 tctatcaatt tcaattatat ctacgccaat atcaaatata ttcattgttc ctccagaaaa
    14041 tttataatac ttctttcaat aattaataat gaatttataa atttactatc gataatacac
    14101 attattattt agtttatgtt acttatctta atttaataat cattataata aaactgtaac
    14161 ttttaaaatt atttgtaatg aatattatat acaaat
//
LOCUS       DAKBNN010000096        14131 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000096 DAKBNN010000000
VERSION     DAKBNN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.096"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(114..1069)
                     /locus_tag="OUB02_002339"
                     /pseudo
     CDS             complement(114..1069)
                     /locus_tag="OUB02_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1437..2225
                     /locus_tag="OUB02_002340"
     CDS             1437..2225
                     /locus_tag="OUB02_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT8886095.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2348..2818)
                     /locus_tag="OUB02_002341"
     CDS             complement(2348..2818)
                     /locus_tag="OUB02_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HCT8886096.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2957..3601
                     /gene="uppS"
                     /locus_tag="OUB02_002342"
     CDS             2957..3601
                     /gene="uppS"
                     /locus_tag="OUB02_002342"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HCT8886097.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3644..4549
                     /locus_tag="OUB02_002343"
     CDS             3644..4549
                     /locus_tag="OUB02_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8886098.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4864..6411
                     /locus_tag="OUB02_002344"
     CDS             4864..6411
                     /locus_tag="OUB02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8886099.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6414..6812
                     /locus_tag="OUB02_002345"
     CDS             6414..6812
                     /locus_tag="OUB02_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCT8886100.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6852..8585
                     /locus_tag="OUB02_002346"
     CDS             6852..8585
                     /locus_tag="OUB02_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCT8886101.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNITQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8817..10028
                     /locus_tag="OUB02_002347"
     CDS             8817..10028
                     /locus_tag="OUB02_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCT8886102.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10221..10481
                     /locus_tag="OUB02_002348"
     CDS             10221..10481
                     /locus_tag="OUB02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCT8886103.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10835..12543
                     /gene="ptsP"
                     /locus_tag="OUB02_002349"
                     /pseudo
     CDS             10835..12543
                     /gene="ptsP"
                     /locus_tag="OUB02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(13335..14000)
                     /locus_tag="OUB02_002350"
     CDS             complement(13335..14000)
                     /locus_tag="OUB02_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886104.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         5691 a         1391 c         2278 g         4771 t
ORIGIN      
        1 attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata caaaataatt
       61 tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa tttctaatct
      121 tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata ttcctcaaaa
      181 ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag aggaaactcc
      241 atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga tattatttca
      301 ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt aggatattta
      361 gtgactggta aatccttaac accatttatt aaactctgta taacacttct ttgtcttcta
      421 tctctttcat ctgtgctatc atttttctta tacgagaaaa tgcaagtgtc tgatatccat
      481 ttaatttttg ctcaccagaa tttcttatat atttaagaga acctttattg tcatttttat
      541 tccatttata agtttcagga ataaatttat ttaattctct tatttcactc ttatcaacag
      601 taacagtaac tccacctaaa gcatcaacta tatccataaa agagaaaaag tcaactacgg
      661 cgtagttttg tatatcaagt tggaagtttt cttctatagt ttctattaat aagttagctt
      721 gtccatatac gtaggcatgt gttaattttt gttctccacg tcctggtata ttaacataag
      781 tatctctatt taatgaagtt agcttaagac ttttattttt attatcaata gtaagtatca
      841 tcatggcatc agaacgagaa acttcttctc ctggtcttgc atctgtacct aacaataata
      901 tgtttgtgat attttcttcg tttttatagt cattactact aagcgcctct gtatttatac
      961 tatcatcttg tatagttttt aatttagtaa aaacatatcc atatatacta acaggaaatg
     1021 ctataactaa aagggctaga aatataacga actttttgag tcctgacaat aatatcccct
     1081 actttctaaa ttttttaatt acacatagtt aattatatta aaaatttgtt acataagcaa
     1141 taaaaaacac aataaattag cataagtttg tcataatata attattttta tagtatttta
     1201 tagaagaaag tgtgtagtta ttattgattt tattggaata taattgtatg aagacttaaa
     1261 tttttataaa ctaatgtgta tattgaaaaa aatattttcc tgaatgtagt tatagcatct
     1321 tatgaaaggt gcaaattgta atttaaaatt tttagtgtat aataagaatt gaaaattcaa
     1381 accgataaaa attaataaaa aataattata aattaataat ttgggggagg aaatgtatga
     1441 taaaattaat agcaacagat ttagatggaa ctttgttaga tgaaaagagc gaaataaatc
     1501 cagagtttta taaagtattt aaaaaattaa gagagagagg gataatgttt tctgctgcta
     1561 gtggcaggca gtatcaaaat cttataaaaa aatttgaaga tataaaagat gatatgatgt
     1621 ttatatcaga aaatggaaca ctagtagtat ataaaggaaa agaaatatta tcaaatcctt
     1681 taaataaaga attagtaaat gaaatcatag agacaacaag aagtataaaa gggaaaaaaa
     1741 ttgtaatgag tgggaaaaaa tacgcttata tagaaagcaa agatgaagcg tttatacaag
     1801 aggtcagtac ttattatgca aagtttaaaa ttgtagaaga tttgacgaaa gtagagggag
     1861 acatattgaa gatagctgta tttgacttca aaggtgcaga acataataat aatatctact
     1921 ttgaaaagtt ttctgataga gctcaagtat gtatttctgg agtggagtgg cttgatttaa
     1981 ctgcaaaagg tgcaaataaa gggagtgcca taaaaaaagt acaaaaaatg ttagatataa
     2041 aatatgaaga aactatggta tttggtgacc agttaaatga tgtggaaatg atgaaaagtg
     2101 cctatcatag ttatgccatg gaaaatgcaa atgaacattt gaaacaaatt gctagattta
     2161 gagcaaaaag aaatactgaa aatggcgttg tagataagat aaaagaggtt ataaaaattg
     2221 gatagtcata tgtatagatg aaaaagatta ttttgaagaa atcaaaaaaa tatcaatgta
     2281 attttgataa ataagtttat ttatgcgaaa attacattga tatttaataa acatcaataa
     2341 tagatattta tttttttatg atattcagta ttttgttttc tatttgagtt gcattttcag
     2401 cagaaattaa ataaacatat ctacctgcct tctcaattat agtattattt gctatttcag
     2461 aattgatttt ttctccatgt ccttctccaa aaggtgcttg tatcatatct tttttataat
     2521 tttctagagt tttatagatt ttatctacct cgtcagtttt tataacgata atatcttcta
     2581 gagataattt ttcagaagaa ttaattacaa aaccttgaat tatgtcttcc tctacatcat
     2641 taaaatagtt aaaatccttt gcatcaaaga tttttgattc ctttataagt aaatcagaag
     2701 attttaatgc tttttttatt tttgatgtag aaacgttttt gtaatcacca cttgaagaac
     2761 aactaaccat tcctaatgat aagattatcg aaaaaaacaa tattaaaatt tttttcatga
     2821 taaacctcca agttttttaa actctgtctt aaattagttt actacataat tatataaaaa
     2881 taaatgtatt taatgttaca gttttgatta ataatgtaat attttgataa taatacctat
     2941 tgagaggtga ttagaaatga gaataccaaa acatgtagga attataccgg atggaaatag
     3001 aaggtgggca agtcaaaatg gtatgacaaa agataaagga tatgaaagtg gaatagaccc
     3061 tggactagag tcttatagat tatgtagaga tttaggctgt gaagaggtga ctttttatgg
     3121 atttacaact gataatacaa gacgtccatc agtgcagaca aaagctttta caaaagcatg
     3181 tattgatgct gcaaatatat taatagatga aggtgctgct ttattagttg taggaaatac
     3241 aaaatcgcca atgtttccag aagaattatt accatatact aatagaatta aagccaaaga
     3301 aggtcaaata aaagcaaatc ttctggtaaa ttatggttgg tattgggact taaataatat
     3361 atcaggagta gaaaatgtaa ataaaaataa tatacaggat tacatacagt catttgatgt
     3421 atccagacta gacttgataa tcagatgggg tggaagaagg agattaagtg gtttccttcc
     3481 tgtacaatca atttatgcag atttttatgt tatagataat tattggccag attttaagag
     3541 tgacgatata aaggacgcat ttagttggta tgatacacaa gatgtaactt taggtggata
     3601 attaaaaata ttctgattta aggttgtaga gtgttttaaa ttattgaaaa aaaaacttct
     3661 tgatggtaaa ataacactaa taatatgtaa aagtgtaaaa atttatacca agaagggtga
     3721 agaaatgtca aaaggcaata ataacaataa ttcaagaaac aaatctaaaa agaccagtca
     3781 tctgaataga aaaaagcgaa aattaaataa gaaaaagtta gcagtgttga tttgctttac
     3841 agttttattt ttatttattg catttaaggc tactcaaggt gtagtagctt tggtaaaaag
     3901 tatggataaa agtaataaaa ctaatcaaca acaaaatgtt aactcagaac aatttgacct
     3961 tggtaatgaa gaggaaaata aaaagaaaaa atatactgta tttatagacc caggtcatgg
     4021 aggcaatgat aaaggaactg aaagcaagac cagtaataga tatgaaaaag acctaaatct
     4081 acaaattgca aaaaaattag caaataaatt atctaaacaa aaggatattc aagtagtagt
     4141 atccagaact gatgatacat atatatcatt aaaggataga gctatcttag caaataatag
     4201 ttctgctgat gtgcttgttt ctattcattt aaatgcagag aaaaatggta atacagcaac
     4261 aggtatagaa acttggtata ggaataaagc tacagatggt tcaaaagaac ttgcacagac
     4321 agtacaaagt actatagtat cctatgttaa agttagagat aggggaatag ttgaaaataa
     4381 ctttgaagta cttagagaat caaatatgcc tgctatatta atagagtgtg gttttttaac
     4441 aacaccttca gaggaacaaa aaataataaa tgaaaaatat caagaccaac ttgcggaagg
     4501 aattgttcaa ggtgtacttt catacctaga ttctaaaggg aataagtaag ttaactttac
     4561 tttaatgtga ggataaattt aaaagtttaa ctgtgtaaat tgtattaact ttatttcgta
     4621 atgttggaaa tattgtttga atttagaaac tatgaaatta gaaattattt tatctatatc
     4681 taatttcata gttttttttg caaaaaattt atagttataa aattttaaaa aataaattta
     4741 gtataatgta atgtagttat ttatatgtga ttttaacttg cattgatttg ctatcaattg
     4801 tgctttataa ttagtatata taaagtttaa ctgtaagata atttaaaaaa taggggaatt
     4861 tatatgaata aaaatagcat gacaagaatt tacagtttaa ataaaataat ttttattata
     4921 attggaattt taggaatagt tttactcaca agacatattg aggtagatat taaagtttta
     4981 attttaattt tattaacctt gtttagtata aatgtattta tttcatatct taaaattaag
     5041 ttgtatgagg ataaaattaa tttaggattg ataaataatt cagataagtt tataaatatt
     5101 actaaagatg agaacttcct aaaacatgtt caaaattata ttatcagtaa tgaaaaagaa
     5161 aactgtgtaa tggcttgttt tgatttatgt agactcaaac ttataaatga tatatatggt
     5221 tatgagctag gggatgaagt gttaaataat attttaagta acttaaaaaa ttattttgga
     5281 aaagaagcta tttatggaaa gttaaaaagt gatgtttttg tattaataat aaagcttgaa
     5341 aatagggaac agaagattcc ttatttagta aatcttatta agaataaaat tgtgatacta
     5401 agtcagattg acagttttaa aatggatata aatattgagg cttctattgg aatttataaa
     5461 tttaacaata atgaaattga tataaaaaaa gctatagata atgctgatat ggcaaggctt
     5521 aaatcaaaag gactaaagca catagagtat gtagaatttg ataatgctat ggaagaagaa
     5581 atacaatcaa ttatgaaaat agagagagat ttattcttgg ctattaaaaa taaagagttt
     5641 gtaattcact atcaacctaa agttgattca agtacaggta acattatagg ttcagaggca
     5701 ttgataagat ggttgcaccc atcattaggc atggttggac caaataagtt tatacctata
     5761 gctgaaaaaa atggtctgat aaacaatatt ggtagatggc taattcaaga agtttttatt
     5821 accattaaca agtggatttc agaaggtata aatgtcttac cagtctcaat aaatctttct
     5881 agagtagaac tatataaaaa tgatttagtt gattttttta aactgatgtt taatacatat
     5941 aatattccaa aagaacttat agaattagaa attacagaaa cgacagcact aagagatgta
     6001 aaatttataa gtgaaaggct ttatgagatt aaatctttag gtatgaatat atcattagat
     6061 gattttggag taggtaattc aaattttatc aatcttaagg ggataccact agatattata
     6121 aaaattgata ggtcacttat attggatata gttacaaata caaaaacaaa gtttatggtg
     6181 aaagcaattg ttaacctttc acatgattta aacgtaactg ttatatgtga aggggtagag
     6241 gatatgcatc aggtcaaagt cttaagtgaa ctgggatgta atttcataca aggttatgta
     6301 ttttttaaac ctttggatga gatgaattat aaaaaacttc ttactgacgg aagttatata
     6361 aatctgaaag atactctttt agataaatgc atgacagttg aggaggagta aggatgattt
     6421 cagaagatgt agattattca ataagaatca taagagctat atttaataat ggagaagata
     6481 aaccaataga tgctaaaatg atattaaaaa gtgaagatat accaatacta acactataca
     6541 gactgcttag aaagctttgt gcaaacaata ttcttacttt taacaaagaa gatggcaaga
     6601 catacatgtt aaatgtgtca ttggatacaa taagtctaag aactattgta gaagtaattg
     6661 atggaaaaat atatataaat aaatgtacga gatatgtatc taattgcagt aattgcttaa
     6721 aaggttgtaa agtaaatcgt gaattgctag aaatagaaaa agaaatttat aaacttttat
     6781 cgagaaaatc tataagacaa atactagaat aaaaaatttt cagctatgat aataattgga
     6841 ggttttcggt tatggatgat atatctcaag ataattttct actgtctaaa gagtatgaga
     6901 atagcttaga tgtagatact aaaaaagcaa gtggaatata ttatactcct aaaataattg
     6961 tagattatat tgtaaaaaaa actttaaaaa atcacgatat aattaaaaat ccatatccac
     7021 gaatacttga tatttcctgt gggtgtggaa attttctttt agaagtatat gatattttat
     7081 atgacttatt tgaggaaaat atatatgaat tgaaaaagaa gtatgatgaa aattattgga
     7141 ctgttgataa catacaccgt catatattaa actattgtat atatggtgct gatattgatg
     7201 aaaaagctat aagtatatta aaagatagct taacaaataa aaaggtagta aatgatttag
     7261 atgaatctga tataaagatt aatctatttt gttgtgatag tcttaaaaag aaatggcgat
     7321 ataagtttga ttacatagtg ggtaatccac cctatattgg tcataaaaaa cttgaaaaga
     7381 aatataaaaa atttctccta gaaaaatata gtgaagttta taaggataaa gctgacttat
     7441 atttttgttt ttataaaaag attatagata ttttaaagca aggagggatt ggcagtgtta
     7501 taactccaag atatttttta gaaagtctat caggtaaaga tttaagagaa tatattaaat
     7561 caaatgtcaa tgttcaagaa atagtggatt ttttaggtgc aaatatattt aaaaatatag
     7621 gggtatctag ttgtattcta acttttgata agaaaaaaac aaaagagaca tatatagatg
     7681 tatttaaaat caaaaatgag gatatatgta taaataaatt tgaaacattg gaagaacttc
     7741 taaagagtag caaatttgaa cattttaata taactcagag attgctatct gacgaatgga
     7801 tacttgtaaa taaagatgat gaaacttttt ataataagat acaagaaaaa tgtaaatata
     7861 gcttggaaga tatagcaata agttttcaag gaataattac agggtgtgat aaagcgttta
     7921 ttttaagtaa agatgatgta aagttgaacc ttgtagatga taaattttta aaatgttgga
     7981 taaaaagtaa gaatataaat aaatatatag tagataaaag tgaatatagg ctaatatact
     8041 caaatgatat agataatgag aatacaaata aaaggatact agatgaaatt atagggctat
     8101 ataaaacaaa attagaaaac agaagagaat gtaaaagtgg aataaggaaa tggtatgaac
     8161 ttcaatgggg tagagaaaag cttttctttg aaagaaaaaa gataatgtat ccttataaat
     8221 caaatgaaaa tagatttgct atagattatg ataacaattt ttcaagtgca gatgtatatt
     8281 ctttttttat aaaagaagag tatttagata aattttcata tgaatatcta gttggaattt
     8341 taaattccag tgtttatgat aaatacttta aaataactgc taaaaaaatg agtaaaaata
     8401 tatatgatta ctatcctaat aaggttatga agataaggat ttttagagat aataattatg
     8461 aagagataga gaatttatca aagcaaataa tatcaatttt attaaataag tctatagata
     8521 aaggaaaagt agaaaaattg cagataaaaa tggataactt gataatggat tctttaggta
     8581 tataagcagt tgtaagtatt aaaaaattat aatcttttaa ttgtttattt aaatttagaa
     8641 ataaaaagtt agccatataa aaaatattaa aaatatatac ttttcaatat ttttttgata
     8701 aaataagaat gaatttgtgc aattttattt ttctccaata actaaaaata gttttttgaa
     8761 ttaatatttg cttaagaata aaaatcagaa acgtttttga tatagtaggt gataatatga
     8821 aaggattagt tttagaaggt ggaggaacaa aaggagccta ccaaataggt gcatataaag
     8881 ctttaagaga gcttggtata gagtttcaag gagtaactgg cacatcaatt ggtgctttaa
     8941 atggagcata tataattcaa aatgatattg aagtaatgga agatatttgg ttaaattatg
     9001 actatactca ttttatgaat attgatagag aaacctatga gaggtttaaa aatgtagatt
     9061 tcactactaa aaatctcaat gcagtgtttg agcttataaa taaagctaga aaaaatgaag
     9121 gtatagatat aagcccatta agacaactaa tggagacaac attaaatgaa gacataataa
     9181 ggaaatcaaa aaaagacttt ggacttgtta cggtctggtg ggataaaaaa attaatccat
     9241 atccattgta tatcgaagat atacctaaag gaagattgat tgattattta attgctagtt
     9301 ccagtttgcc tatttttcaa acgaacatga tggatgataa gttgtatctt gatggaatgt
     9361 tttgtgataa catgccaata ggactcttgg aagacaaagg ttatgaagat gttgttgtaa
     9421 ttaggttgat tgatgatttt attgggaaaa tgaatctaaa tcgacatcaa aatataaata
     9481 taaaaactat aataccatca gaatatctag gaggttctct taataaagat agagataatg
     9541 tagaaaaaaa catccgacta ggttacttag atactatgaa agcatatgaa agatgcactg
     9601 gaattaagta ttattttgat gtagagtata aatataatga agaatattgt tttgataaat
     9661 ttagaagtat gagcaatgaa acaatacaat atatctgtaa tttattgaat ataaaaagag
     9721 atgcttcact tagaactgtt atggaaagtg taataccaaa gcttggtgag gtgcttggat
     9781 tgccaaagga tttttcatat aaagatttgt tttactgtat atatgaaaag aaacttgaag
     9841 aaaataatat aaacaggata aaattatatg attttaacaa ggttatagat gttgtaaata
     9901 caaatattga ttctaataga aatttagctt ctaattttga agttagacct aaaattagta
     9961 ttaaaaagaa caaaaacaca aaattattga caaattgtat tataaaagac ataaaaaatc
    10021 aaagttaaat aaaaaacaaa aaaaaatttg taattatact cgaaaaaagt gtatattagt
    10081 agtataatat ttttgtagca ctagtgggaa caaaaaatga ccataggaca aaaaagtacc
    10141 acttagtacc attaaagtaa aaaacaggaa aacaatttta aaaaatataa aatgtaaagt
    10201 ttataggagg atttaaaaaa atggaaaaag tagttagtat caaaaatgca agtggattac
    10261 atgctagacc agcaggaatg tttgttaaga aggcagctga atttaaatca acagtagagg
    10321 ttgtagcaaa agacaaaact gtaaatgcaa aatcaataat gggaataatg agtttaggtt
    10381 tagctcaagg agaagaatta aagttagttg taaatggaga agatgaagaa gtagctttag
    10441 ctgcaatggt tgaattagtt gaaagtggat ttggagaata gatataataa ttttttaaaa
    10501 atttgatatt tagaatctaa tattacttaa agtaaagtaa cagagaagta ttaaacatat
    10561 acagaccaac atttgtagat atttaatttc tttgttactt tcatgtttat ttaatcagaa
    10621 gattaagact attgttaatt gggtataaaa atataaatat tttagaaaat gaaataagat
    10681 atttttaatc ataaattcag agtaaaaata cagtaacaaa tagtttataa aattttataa
    10741 aaaattataa gtaaataaaa ctataaaaat gtaaattatt tataaaattt tatagagttt
    10801 atgtgctaga tgatattttt taggaggtag ggttatggct tacaaaggaa taggtgcttc
    10861 tcctggtgtt gctttaggga aagcgttagt agttgaacat agtgaattag taattgaaaa
    10921 aaaatctata gataatgttg aagctgaaat tgctaaatta gaaaatgcag ttgcagtttc
    10981 taaagaagaa ttagtaaaag ttaaggaaaa ggcttctgaa gaattaggag cagaggaagc
    11041 agaaatattt gaagcacatt tattagtctt agaagaccca gaattaattg gttctgcaat
    11101 agataaaatc aaaactgaaa gtgtaaatgc agagtatgct ttaaatgaaa taaaagagat
    11161 gtttgttagc atgtttgagt ctatggacaa tgagtacatg aaagagcggc agatataaaa
    11221 gatgttacaa atagaatact tagacatata ttaggaatta aagttgttga tttatctgct
    11281 ttaagcgaag aagttgtatt aatagcacat gatttaactc catcagacac agcaactatg
    11341 aataaaaaaa tggttttagg atttttaact gatataggtg gaagaacttc tcatactgct
    11401 atcatgtcta gaactttaga aatagctgca atcgttggat taaatgatat aacttcaaaa
    11461 gttaaggatg gagattttgt agttttcaat ggagacacag gtgaagtaat cgtgaatcct
    11521 gatgaagaga ctataaataa atatactgaa ttaaaagcta agtatgaaga tgagagaaaa
    11581 gcattacagc tattaaaagg aaaaccatct gtaactttag atggaaaaca tgtagaatta
    11641 gctggtaaca taggaacacc taacgatata gaaggtctaa taaagaatga tgctgaaggt
    11701 gttggtcttt atagaacaga atttttatac atggatagag attctttccc aacagaagaa
    11761 atacaatatg aagcatacaa agctgtatta gaaggaatgg atggaaagcc tatagttata
    11821 agaacattgg atataggtgg agataaagaa ttaagttatt taagtatgga acctgaaatg
    11881 aatccattct taggttacag agcaataaga ctttgcctag atagaaaaga tatatttaag
    11941 actcaattga gagctttata tagagctagt gttcatggaa gattaagaat catgttccca
    12001 atgatttcat ctttagaaga gttgttacaa gctaaagaag ttgtaaaaga agttttagca
    12061 gaacttgatt ctgaaggtgt ggcttatgct aaagatgtag aaattggtat gatgatagaa
    12121 gttccatctg ctgctgtgat atctgatgta ttggctaaac acgtagattt cttctctata
    12181 ggaactaatg atttaattca atacacttgt gcagttgata gaatgaatca aaagataagt
    12241 tatctttaca atcaatttaa tccagctgtt ttaagactta taaagactgt tatagacaat
    12301 gcacacaaag aaggaaaatg ggcaggtatg tgtggagaat ctgctggaga ccaaaaaatg
    12361 ataccaatac ttttaggtat gggattagac gagttctcta tgtctccaat atcaatactt
    12421 cctgctagaa agttaataac ttcagttaaa gagtcagata tgaaaaaatt agctgatgat
    12481 gtacttaata tgggtactgc tgaagaaata aaaagctata tagaaaagac ttttaatata
    12541 taattaatgt tatatagtta ctaaaattca tttttctaaa taacaaaatt atattttacg
    12601 aattaaaaag aagatgtttc aaaataaaaa tttgaagcat ctctttttta taaaagatta
    12661 ctaagaatta cttaatcttt gtaatagtca ttttgtgaaa aagcaaaact gcattgtgac
    12721 tatagtatat atggatgtta ttatcaataa aagtactatt aaaatgaaga ttcattctca
    12781 aatggtatca aaagtagttg tgatggagta agataatatt ttgtgattta atagtaaaaa
    12841 ttaaaaaaat atggtattga aaaataatta aaagtattta ataatagaat tagggaagaa
    12901 taagtttata agtaaaaata attgattagt agttaataaa ataaatgtga tatccaaata
    12961 aatcctaaat ctaatttgag ttttaatata gatagtctaa agtttgatta ttaaagattt
    13021 aattgtaagt tgttacagtt gatgatattg attagagatt aaagtttaga gtagaaagtt
    13081 accttaaatt taaaaatgca agataaccca atctaaaaat tataatcact cgataggttt
    13141 aaggatttat ttgtttatta taaataggag ggtgtctcaa aatgaacttt ttagtttatg
    13201 aggcactctc ttttatatag aattaaatat attttattat ttcaacaatg aaaaagaagc
    13261 ttatctctat tttgagacag cctctttgta tactattatt aatttaatca ataacataat
    13321 ttaaaattat attattagtt aagatttcct gctttattag taaataattc tttaatagca
    13381 ttgtttctgg aaactaaatc gttatcagag ttgtttgttt ttttcataat cactgaataa
    13441 cgttttttat ttataaaaaa tattaaaatc ttatcatcat caaaattgta ttctttatat
    13501 ctgtcaatag actcataacc tagatttaga atgtttttgt cttttatact atctacatct
    13561 aaaatatatg atgggtcagc ctttagtaat atattaatta aacatttttc ttcaccagca
    13621 cagaataact ccatcttact tgatatagat ttagattgct ctgaagttat ttttgcagga
    13681 ataaggttgt cattatcatc tcttagaaga taaccatgtg tgtcttgttg atatactaaa
    13741 ttaacctttt tcttaggaac actaatcatt ttatctaaat gtacataatg atgattttca
    13801 acaaaacctt catttaatat ttcaacaaag ttattaattt tacctaggca aaaagattct
    13861 atagattctt tacagaattt atttgtaaat ggtattatgt ttacgttttc ctcgtctgta
    13921 ttccatgcta tagcataatc caacgaatat acttcttcct ctatatctgc aaaattatat
    13981 tgtactatgt ttcctttcaa aattactcga cctccatctt ataatatatt taatatactt
    14041 tataaaactt atattatatt taaataatat cataaattgg ataaatatga tatagtaaat
    14101 aagaaaaaat ttgaaaaaaa tcactataaa t
//
LOCUS       DAKBNN010000097        14059 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000097 DAKBNN010000000
VERSION     DAKBNN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.097"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            445..1515
                     /locus_tag="OUB02_002351"
     CDS             445..1515
                     /locus_tag="OUB02_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886105.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1499..2368
                     /locus_tag="OUB02_002352"
     CDS             1499..2368
                     /locus_tag="OUB02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCT8886106.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2412..3272
                     /locus_tag="OUB02_002353"
     CDS             2412..3272
                     /locus_tag="OUB02_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCT8886107.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3303..4607
                     /locus_tag="OUB02_002354"
     CDS             3303..4607
                     /locus_tag="OUB02_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8886108.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4670..5932
                     /locus_tag="OUB02_002355"
     CDS             4670..5932
                     /locus_tag="OUB02_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCT8886109.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKDGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6119..8089
                     /gene="uvrB"
                     /locus_tag="OUB02_002356"
     CDS             6119..8089
                     /gene="uvrB"
                     /locus_tag="OUB02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCT8886110.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8108..10933
                     /gene="uvrA"
                     /locus_tag="OUB02_002357"
     CDS             8108..10933
                     /gene="uvrA"
                     /locus_tag="OUB02_002357"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCT8886111.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11192..13009
                     /gene="uvrC"
                     /locus_tag="OUB02_002358"
     CDS             11192..13009
                     /gene="uvrC"
                     /locus_tag="OUB02_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCT8886112.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13009..13938
                     /locus_tag="OUB02_002359"
     CDS             13009..13938
                     /locus_tag="OUB02_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCT8886113.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
BASE COUNT         5717 a         1615 c         2553 g         4174 t
ORIGIN      
        1 gggtaaattt ataaaaatta aaaaagtttt attttttatg tttaaacctt gtaaatttgg
       61 gacataatat aattgtaaaa cttaatatag caaaaataaa ttactcaatc tcccccactt
      121 aaagacctct atcccccata gaggtctttt ttactttcaa atcaaattat ataaaaaata
      181 aatagtttta tatgactctt cataaattaa aattacattg tgatagcaaa ccatgaatat
      241 gaaaaatata gaaaattata gctaacataa gctgtatatc atcttaacct cagtatattt
      301 aacaaaaaat gtttataatt taacctttat tttaataagt ttaccatata ttcactttgt
      361 cttaatatat cctttacaaa ttaagagtat tattaatcta taagtaaatc gtagaattaa
      421 attaaatatt ttgggaggat aaaaatggca gatataagac tagaaaatgt aacaaaatct
      481 tataataaaa aaaatacagt tatagaaaat ttaaatctta ctatagaaga tggttctttt
      541 acagtattgg taggcccaag tggatgtggt aaatctacta cgttaagaat gattgcaggt
      601 cttgaagata taacttcagg taggttgaag attgaagaaa atgatgttac aaaaacggat
      661 gctagtaaaa gggatatagc tatggtattt caaaattatg cattatatcc acatatgact
      721 gttagagaaa atatagaatt tggtcttaaa aacaaaaaag tagaaaaaga aaaaagagaa
      781 aaacttataa cagaagttat tgatgtagta ggattgagag aatatttaaa tgtgaaacca
      841 ggcaacttat ctggaggtca aagacaaaga gtggcacttg ctagagctat ggttaaaaat
      901 ccaaaggtat ttttaatgga tgaaccattg tcaaatttag atgctaaatt aagaaatcaa
      961 atgagaacag aattaataca attacatcaa aaactaaagt ctacatttgt ttatgtaact
     1021 catgaccagg ttgaggcaat gtcaatggca gataagatag ttataatgaa caaaggaaag
     1081 ataatgcaaa taggttcccc aaagaagata tatcaaaatc ctaaaaattt atttgtagct
     1141 caatttatag ggagtccttg tatgaacata ctagatataa aaaatgatat taaatttgga
     1201 ttcaggccag aaaaagcaac actagagagt ataaacatag atgggaaaaa tgatgatgta
     1261 tttagcctat ttggaaaagt tgtgactaaa gaagtgttgg gaaatgaaac tatatattgt
     1321 ttatctgttc ttaatagaga gataagagtc aaaacggaaa atgatatatt tgatattgga
     1381 agaactttaa gaataagtgt attagaaaaa gatttatatt attttgataa aaatggagaa
     1441 agaatagaag atgtaaaagt atgtaaaagt atgtattcta aaattaaagg agatatttat
     1501 gaaaaagcaa tttaataaag catatttata tataacacct gctattatag gaactttatt
     1561 gtttatagtc tatccaataa ttaagacgat atctctaagc ttcaaatctg gtagtcttct
     1621 tagttcaacc tttgaaaatg taggcttaaa caattataca gaacttttaa agaatccaga
     1681 gtttatacaa actataacga acactgcaat ttatactttt tttatggtga ctatatctat
     1741 atcactagcc atagtacttg ctgtttggct aaataaaaat tctataatac acaatttaac
     1801 gcaaagtata atctttacac cacatgtaat ttcactagta tctgtagctg tattgtggct
     1861 ttggattatg gagccacaat atggcttttt aaactggata ctatcgacat tacatttacc
     1921 aacatcaaag tggctttcaa gtgaaagttc agctttaatg tcactaattc taataggaat
     1981 atggaagact cttggataca atatcttaat agtgttatca ggtcttaaat ccataccaaa
     2041 ctatatatat gaagcatcta aattagataa tgcaaacaaa ataacgaatt tctttaaagt
     2101 gacattgccg ctattatcac caacaatatt ttttcttatg ataacaacaa caactgcatc
     2161 ttttcaagta tttgatgaga taagtataat gacacaaggt gggccagtgg gttcaactaa
     2221 catgttatct tattatatat atgagtctgg atttatatac tatgacatag gacaagcgag
     2281 tgcagcgagt atgtttttgt tagctatagt tatgattgct acatatgcta actttagact
     2341 cttatcaaag aaagttcatt atcaataaag aaaaagtaga tattataagg tttatccaaa
     2401 aggaggagac aatggatatt atacaaccta tcaaaaaaga caaagttgat ataaaaaaaa
     2461 tagcatcaaa atcaatagaa atattaattc tattactttt atctgcaata tttatattcc
     2521 cttttatatg gatgctatct acatccttta aaaatccaca agaaatgatg caattaccgc
     2581 ccactattat accaaatgat atagtgttag aaaattatag tacagcatgg cattcaggtc
     2641 cattttttag atatttgcta aacagtgtat ttgtaacagg aagtgtttta attgtacaat
     2701 ttttagttat ggttccagca gcatatgcat tttctaagat taaatttaaa ggtgaaaaaa
     2761 tgctatttgg gcttttgctt gtaggactta tgattccacc acaagtaact tttctaccag
     2821 tctatatgat gatgagtaag tttggattta taaatactta tgttccactt ataattccat
     2881 ttatgacatc ttcatttggg atatttctgc ttagacagaa ttttatgcag atttcagatg
     2941 aaattattga agcagctaaa ctagataatg cttctgactt aaaaataatg tttagaataa
     3001 tggttcctat ggcaaaacca gcagtaataa catttatatt atttaatttt atataccatt
     3061 ggaataatta tttctggccc ttagtaatga caaatacaga tgcaataaga actttaccaa
     3121 taggagttgc gctccttaaa tcttcagaag gaattactgc atggaatgta ataatggcag
     3181 gaaatataat tctaatttta ccaataattc ttgtttatat atttgcgaat aaaaaagtaa
     3241 aagaagcttt tatgtattca ggaataaaat aaaaaattaa agactaaatt tggaggatga
     3301 aaatgaagct taaaagaaaa ttagcaacag gattagcaat aacattatta ttaggttctt
     3361 tagttgggtg ttcaaattct aatgatgcaa atgcatctaa aggagaaaag actgatagca
     3421 atattacgaa gatagatttc tgggctcctt taggtggaac taatggagaa actgctcaaa
     3481 aaatggttga ccaatttaat agtgaacata aggatgtaca agtaaatatg ttaaaacaga
     3541 aagattacta tgaaaatgca acaaaactac aggcagcctt aacatctaaa gaccaaccag
     3601 atgtaacatt attagaaata actcaaacag gtacattcgc atcagcaggt gctttagtag
     3661 atatgagtaa atattttgat aaaacatatc aagaaagatt cttttctggt cttttaacaa
     3721 attcatatta tgaggataag tttgttggta tgccatttaa tagaagtaca ccaatattat
     3781 acataaataa agatatggca actaaagcag gtcttgaccc ttcaggtcca aaatcatggg
     3841 aagagcttaa ggattatgct agtaaaatga caaataaatc agaagatact tatggttttg
     3901 aaacgccaat agatatttgg ttctatgaag ctatggtaat gcaaagtgga ggagaaataa
     3961 ctaatggtaa aaaagtagca tttaataatg aggcaggaca agcaccagtt aagttctggc
     4021 aagatatgat gaaagctgga ataatgaaaa tgcctcctgg agaagattat aatgcctggg
     4081 acgtagcaaa acaagatttt gtaaatggta aagtaggtat gatatttaca tcaacagcag
     4141 atttagcagg acttatgcaa caaacagaag gtaagtttga aatatcaaca gcattcttac
     4201 caaaaaatca aaaatacgca actcctacag gtggagctaa tctagttatg cttgaaggtg
     4261 gaacagataa agaaaaagaa gcaagtgcag aatttataga atggatgaca caaacagata
     4321 aaatcgttca gtttagttca tcaacaggat acttaccaac aactgaagat gcaacaaact
     4381 cagaaaagtt acaaacatta tataaagaaa aacctcaata taaggttgca actgaccaat
     4441 tacaatatgc tgttgcactt ccaatgttaa atggatacaa ggaagcaact gataagctaa
     4501 tggatgagat taaaaagggg cttactgatt taaatgcaaa tccaaaagat gtagtaagca
     4561 aaggaacttc tgcaatgcaa gcagtaatag ataaaacaaa taaataatta tatagacttg
     4621 taagttgatt aaaagtattc aataatatat aaatgaagag gtaatgtaaa tgacaaaggt
     4681 tatagtatta tcagtagatt ctttatttga aaaagacttg gaatttgtaa aaaatctacc
     4741 aaactttaaa tctatactag aaaattgctc catagtaaaa aatataaatt gtgtttatcc
     4801 cactctgaca tatccgtgtc atactagtat ggttacagga gtatatccta aaaaacatgg
     4861 tatatatcat aatgaaaaat ttgacccaaa taaagaaaat aaagactggt actggtattc
     4921 aaaggacata aaagcaaaaa ctattataga tgtagcaaaa gaaaataatc taactacctc
     4981 tagtgtatta tggcctgtaa tgggagcaaa tccaaatata gattataata ttgcagaaat
     5041 atgggctcct agtagagaag atgacccaag agaaactttt gaacgaagtt ccagtaaggt
     5101 tataatggac ggtatttaca atagacattg tcattatatc gattggaaat tagaacccaa
     5161 catggattta tttggagtaa actgttcagt agatataata aaggaatata aaccagattt
     5221 aatgctaata catttagcta cattggacca tacacgacac aattatggct tatttaatga
     5281 cgaagtagat aaagcattaa aaatgaatga taaatggtta ggagatatta ttcaggctac
     5341 aaaggatgca ggtacataca aagatacaaa ttttataata cttggggacc atggtcatct
     5401 tagagttgat aaagttataa atccaaatgt cttgcttaaa tcaaatggat ttataaaagt
     5461 tgaaaatgga gaagttaagg attttgatgc ctatgtaaat tcttctggaa tatcagctca
     5521 aataatagta aataacttag ataggttaac agaattaaga gaactgcttg aagaatttaa
     5581 agaagaatta ttcatagaga atatttttac caaagaggaa gcatctaacc ttggtcttga
     5641 aggtgatttt gaaatggtaa ttgaaggtct tgaaggaatt tcatttggaa atgatttcga
     5701 aggaagtatt ataaaagatt cagatataaa agattacaag tttgctgttg cgacacatgg
     5761 tcatttacct actaaaggta atagacctcc atttatagct tttggaccaa atattaaaga
     5821 tggtgtggtt atagaagaag gagacctgag agcacatgca gcaactatat taaaaatgtt
     5881 taatttaaaa ttagatggag ttgaaaaaga agcatttgat tttattatgt aagttgaata
     5941 aagtatttat tttccatgaa ttatagactt ctattgcaaa gatagaagtc tttttttatg
     6001 ttttagattt aagtaaaatt taaatgggta aaatatttaa aataacttaa acaattttat
     6061 aaaggtatga tataataata catgtcagga actaatgttc gaaaaaagga gtgatactat
     6121 ggattttaaa ataaaatcag acttcaaacc tacaggtgac caaccagaag ctattaaatc
     6181 aatagtggat tctataaata gaaatgagaa attttcgaca ttattaggtg taacgggttc
     6241 aggaaaaaca ttcacaatgg caaatatcat tcaacaagtt aagaaaccta ccctaataat
     6301 ggctcataat aaaactctag cagcacaact ttacagtgag tttaaagaat ttttcccaga
     6361 caatgcagtt gaatactttg taagttacta tgattattat caaccagaag catatgtagc
     6421 tcacagtgac acttatatag aaaaagatgc aagcataaat gatgaaatag ataaacttcg
     6481 acattctgct acagcatcta ttttagagag aagagatact ataataatat catctgtatc
     6541 ttgtatatat ggacttggtg acccaaaaga ctataaagaa cttatgttat ctataagacc
     6601 aggtatgcaa agagacagag atgatgtaat aaaaaggtta atagagatac aatacgaaag
     6661 aaatgacata aactttacac gaggtacctt tagagttaga ggagatatac tagaaatatt
     6721 cccagctagt aatgatgaaa aagcaataag aatagaattt tttggtgatg aggtagatag
     6781 aataacagaa attgattatg taacagggaa gattgttggt actagaaatc atgttgttat
     6841 attcccagca tctcactatg taacaacgcc agaaagaatt gaaaaggcaa ttgtagaaat
     6901 agaaaacgag cttcaagaac aaattaaatt tttcaaggaa aatgataggc tattagaagc
     6961 acaaagaatt gaacaaagaa ctaagtatga tatagaaatg ttaaaggaaa taggattttg
     7021 tcaggggata gagaactatt ctagacatat tacaggaagg tcagaaggag aaagaccata
     7081 tactttaatg gatttcttcc cagatgatta tttgataatt gtagatgagg cacatgtcac
     7141 tataccacaa gtaagaggta tgtatgcagg agacaggtct agaaaaacat cacttataga
     7201 gaatggtttt agattacctt ctgcacttga caatagacca ttaaattttc aagaatttga
     7261 aggaaatata aatcaaatgt tgtttgtaag tgctacacca ggaccttatg aaattcaaca
     7321 ctcagagaca atagcagaac agataataag accaactggc cttttagacc caattgtaga
     7381 agtaagacct ataaataatc aaatagatga tttggtagga gaaataacta aaactataga
     7441 aaaaaatgaa agagtgctta taaccacttt aactaaaaaa atgagtgagg acttgactaa
     7501 ctatcttaaa gaaataggga taaaagttaa atatttacac tcagatatag ttacattaga
     7561 aagaacagaa ataataagag atttaagatt agggaagttt gatgtccttg taggaataaa
     7621 cttacttaga gaaggattgg atataccaga agtttcactt atagcaattt tagatgcaga
     7681 caaagaaggt ttcttacgtt ccgaaacagc tttaatacag actattggac gtgcagctag
     7741 gaatgaaaat ggaagagtaa ttatgtatgc agacaggata actgactcaa tgcaaaatgc
     7801 tattgatgaa acaaagagaa gaagagatat acaaaattta tataatgaag agcacaatat
     7861 cattcctaaa actatacaaa aaaatataag agatagtata gaagctacta aggttgcaga
     7921 ggaagaagtt gtatatggaa tatctgatac agatgataaa gacgaaataa gagctaatat
     7981 agataaatta aaatcagaga tgatggaagc tgctcaaaac ttacaatttg aaagagcagc
     8041 agaacttaga gataaagtga aacaattaga agaaaaattg gaaaaataga aaggtaaaaa
     8101 caacattatg gaagataaaa ttataataag aggagcaaaa gaacataatt taaaaaatat
     8161 agatttagaa ttaccaagag ataaatttat tgtttttaca ggtttgtcag ggtctgggaa
     8221 gtcttctttg gcatttgata caatatatgc agaaggacag agaagatatg tagaaagtct
     8281 atctgcttat gcaagacaat ttttaggtca gatggaaaag ccaaatgtag aatatataga
     8341 gggtttatca ccagctatct caattgacca gaagactacc tcaaaaaacc cacgttctac
     8401 ggttggaaca gtaacagaaa tatatgatta tttgagacta ttatttgcaa gagtaggaga
     8461 tgtgtattgc cctacatgtg gtgaaaaaat aagtcagatg actattcaag aaatagttga
     8521 taaaatgcta gattttccag atagaacgaa attacaaatt ctttcaccaa ttgttagagg
     8581 acaaaaaggt acacataaaa aagtctttga taatatcaaa aaagaaggat ttgtaagagt
     8641 tagagttaat ggtgaaaact atgaagttag tgatgatata atcttaaata aaaatcaaaa
     8701 acataatata gaagttgtag tggacagaat agtaattaaa gatggaatag aatcaagact
     8761 agcagattca attgaaactg cagttaagct atctgatggt cttgtaatag cccaaataat
     8821 agatggagaa gaaattcttt tttcaactaa atttgcatgc ccagaacatg gaattggcat
     8881 agaagaatta tctccaagaa tgttttcatt taatgctccc tttggtgctt gtgatgtttg
     8941 taatggtctt ggggaaagta aagaagttga cccagaatta gtagtaccaa acaaagattt
     9001 atcaataaaa caaggtgcag tggcagcatg gggttcaaca ggtgttaatg atgacactta
     9061 ttacagtaaa atgataaaaa gtttagcaga gcattttaat gtatctttga caacaccgtt
     9121 taaagattta ccagatgatt ttgtggagga attactttat ggtaaagata atataattgt
     9181 agagtttact tatgagtcaa aatttggagg aactagaaat tataaatcat actttgaagg
     9241 tgtaatagta aacttagaga gaagatatag agaaactaat tcagattaca tgagagataa
     9301 gattgaagaa tacatggcag agagaccatg tccaaagtgt aaagggatga gacttaaaaa
     9361 ggaagtttta tctgttttag ttgatggaaa aaatataatg gaagttacaa acttatctgt
     9421 aaatgaattg attgacttta tggaaaatat aaatcttaca gaaaaacaga gatttatagc
     9481 tcatgagatt ataaaagaga taaaaggaag agctatgttc ttaagagatg taggtcttga
     9541 ttatttaaat ttatcaagaa aagctggaac tttatcagga ggagaagcgc aacgtataag
     9601 attagctact caaataggtt ctgcattggt tggagtttta tatgtacttg atgagccaag
     9661 tatagggctt caccaaagag ataatgatag gcttataact actcttagac atctaacaga
     9721 tttaggaaat accttaatcg ttgtagagca tgatgaagac acaatgagag aagctgatta
     9781 tgttatagac attggtccag gtgcaggtat acatggtggt gaaatagttg cacagggtac
     9841 tttagatgaa ataattgaaa atgagaactc aataacgggt caatatttaa gtggtaaaaa
     9901 acaaatattg cttcctgaga ctactagaga aggaaatgga agttttatag aaatagttaa
     9961 agcatcagaa aataatctga aaaatattga tgtaaagttc ccattaggta agtttacatg
    10021 cataacaggt gtttcaggtt ctggaaaaag tacattaata aacgatatac tttacaaagg
    10081 tgttgcaagt aaagtgaata gacttaagca aagagctggt aagcataagg agataaaagg
    10141 aattgaaagt atagataaag ttataaatat agaccaaagt cctattggaa gaacacctcg
    10201 ttcaaatcct gctacttata caggtgtatt tgattttata agagatattt ttgcatctac
    10261 taatgaagct aaagcaagag gttataaaaa aggtagattt agttttaatg taaaaggtgg
    10321 tagatgtgaa gcttgtaagg gtgatggaat aataaaaata gaaatgcatt tcttaccaga
    10381 tgtatatgtt ccttgtgaaa tttgtaaagg tgaaagatac aatagagaaa cattgcaagt
    10441 aaaatacaaa gataaaacta tatctgatat attagatatg aatgtggaag aagctgttga
    10501 gtttttcgaa aatataccaa atataaaaag aaaattagaa actttaatgg atgtaggact
    10561 ttcatatatt aaattaggac agccttcaac tcaattatca ggaggagaag cacaacgtat
    10621 aaaattagct gctgaactta gtaaaagacc aacaggaaag actttgtata tattagatga
    10681 acctactact ggacttcata tggcagatgt agacaagcta atccaagtat tacaaagact
    10741 agcagataca ggaaatacaa ttatagtcat agaacataat ttagatgtta taaagacttg
    10801 tgattatgta atagacttag gaccagaagg cggagataag ggtggaacta tagttgcaac
    10861 tggaacacca aaagaagtat ctaaagttga tggttcttat acaggtcaat tcttaaagaa
    10921 atatttttct taagttatat aaattgtgaa aaattttaga gcagatatac gataaactgt
    10981 tttgttataa gtaaattagt caaatataag ttgtaaatag taatacaaag taaagtccat
    11041 attactcttc atggttaaaa ttgttttcaa atcaattata gaagggaata tgggctttaa
    11101 cttgtctaaa gcactggaaa ttgtataact ttttaaaatt catattttag ggtataattt
    11161 tagtataaaa tttaatgttt tggagggcga gatgtttgat atacaagagc atttaaaaaa
    11221 attaccttct gagcccggag tatatttaat gaaagacaaa tacgaccata taatatatgt
    11281 gggaaaagcc atatctctaa aaaatagggt aaggcagtat tttcaatctt caaaaaacca
    11341 tacatctaaa gtaaaatcta tggttaagaa tatatataag tttgagtata ttataacgga
    11401 ttcagagtta gaggcattaa tacttgaatg taatttaata aaaagataca gaccaaagta
    11461 taatgtagtg cttagagatg ataaaactta tccatatata aaagtaacta caaatgaaga
    11521 ttatccaaga atcttaaaag ttagaagagt tcttaaagat aaggcaaagt attttgggcc
    11581 ctataccaat ataactgctg tgaatgatac cttggagctt ataagtagta cgtatccaat
    11641 aagaagctgt aaaatagata tagataaagc aataaagaat aagacaagac cttgtttaaa
    11701 tctacacata aataaatgtt taggaccttg tacaggaaat gtttctaaag aagagtatgg
    11761 aaaaatgata gaagagatta taatgtgttt atctggtaaa gaagaaaaat tgatggaact
    11821 tttaaaggaa aaaatgaatg agagttctat gaactttaga tttgaagagg ctgctgtata
    11881 tagggataag ataaagagcc ttgaagagat gatacagaaa caaaaaatag atgctactgt
    11941 aagtgactta aaccaagatg tggttgcaat ggcaagagct cataatgaag cttgtgtcca
    12001 ggtatttttt ataagaaatg gtaagattgt aggtagggaa cattttatat tagaaggggt
    12061 aatggatagt ccaagagctt ctatattaag ttcatttgta aaacaatttt ataatgaaca
    12121 agagtatata cctaaagaat taattataga agatgagata gaagatagtt ctatattaga
    12181 agaatggctg tcatctaaaa aggggcaaaa agtaacaata agagttcctc aaaagggaga
    12241 gaagaaaagc ttagttgaaa tggttaggaa aaatgctgta gaatatcttg aaaaattttc
    12301 tgatatgaat aaaagaaaat atgaaaaaag tattggagca ttggaagaat taaagcaaat
    12361 attaaatcta gaaaagttgc caataagaat agaggcttat gatatatcaa atatacaagg
    12421 tgtagattcg attggttcta tggtagtgta tacgaatgct aaaaaagata agaaggaata
    12481 taggagatat aaaataaaga cagttattgg acctaatgat tatgattcta tggctgaaat
    12541 tgtagataga agattaaaac atggaaattt acctgacttg atattgttgg atggtggaaa
    12601 aggacaagtt agtgcagtaa aaaaagtact agaactcaat gatgtagata tacctctttg
    12661 gggaatgtac aaagatgata agcatagaac taaaggattg atatgcaaag aaaaagaaat
    12721 agaactagat aaaacaacta atttatatag atttattgct agtatacaag aagaagttca
    12781 taattatgca ataacatatc atagaagtct aagaaataaa gctttgacaa aatctatctt
    12841 agatgatata caaggaatag gtgaaaaaag aaagaaatca ttattaaatc actttaaaga
    12901 tgtagatgct ataaaaaaag ctactatgga agaactttta gaagtagatg gaatgaataa
    12961 aagcattgca gataatgttt ataatttttt tagaaaggaa gaaaattaat ggaaactagc
    13021 aataaagtat caataagaga gataataaaa gagttgaagt taaaagtagt atacatgcct
    13081 gatgatgtag attattatgt ctactctcaa gatataaata gaccaggatt acagtttgca
    13141 ggatattttg attattttgc atatgaaaga atacaaatag ttggaaagac agaatataat
    13201 ttttttggat taatggatag tcatattcgg gaagaagtac tagatagatt tttctcatat
    13261 gaaataccag cgttaataat ttctagagac ttggaaatca agccagatgt aattgaaaag
    13321 gctaaaaaat ataaaagaat acttttgagc acaaatagaa ataccactag acttataaat
    13381 agactgtcaa actatttgga taataagtta gcaccacata caacaattca cggtgtgcta
    13441 gtagatattt atggtatagg tgtactgata aaaggtgaaa gtagtatagg aaagtcggaa
    13501 acagcattag aactgattca aaggggtaat agattagttg cagatgatgc tgtagaaata
    13561 agaaaattag atgaaagtat gcttgtgggt cagtcacctg aactaataag acattttctt
    13621 gagataagag gaataggaat aatagatgtt agaagtttat atggagtagg ggcaatcaaa
    13681 aattctaaaa tgatagattt agtagttcat ttagaagctt gggatgaaac taagtattat
    13741 gatagactgg gactggataa agaatatgaa gaaatacttg gtgttaatgt agagaaatta
    13801 gttgtacctg ttaaacctgg aagaaatact gctatgatat tagaagtagc agcaatgaat
    13861 tttagacaaa gaggaatggg atacgatgca gctcaagaat ttactaaaaa attatcaaaa
    13921 ttaatcgata ctaagtagta ctgaataata gtggactaca catcatttaa ttaaatattg
    13981 attaggatta tgtcctacac cttataaatt aaaatcaagt tgtatatata atgtttatta
    14041 ataagatata ttatataag
//
LOCUS       DAKBNN010000098        13903 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000098 DAKBNN010000000
VERSION     DAKBNN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.098"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            566..1459
                     /locus_tag="OUB02_002360"
     CDS             566..1459
                     /locus_tag="OUB02_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HCT8886114.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1459..2853
                     /gene="gltA"
                     /locus_tag="OUB02_002361"
     CDS             1459..2853
                     /gene="gltA"
                     /locus_tag="OUB02_002361"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HCT8886115.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3219..5438
                     /locus_tag="OUB02_002362"
     CDS             3219..5438
                     /locus_tag="OUB02_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8886116.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5585..6331
                     /locus_tag="OUB02_002363"
     CDS             5585..6331
                     /locus_tag="OUB02_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886117.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGESIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6331..8211
                     /locus_tag="OUB02_002364"
     CDS             6331..8211
                     /locus_tag="OUB02_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886118.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFLEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8330..9721
                     /locus_tag="OUB02_002365"
     CDS             8330..9721
                     /locus_tag="OUB02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8886119.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9828..10868
                     /locus_tag="OUB02_002366"
     CDS             9828..10868
                     /locus_tag="OUB02_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCT8886120.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     IEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11357..12010
                     /locus_tag="OUB02_002367"
     CDS             11357..12010
                     /locus_tag="OUB02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCT8886121.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFISKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            12086..12295
                     /locus_tag="OUB02_002368"
     CDS             12086..12295
                     /locus_tag="OUB02_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886122.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12435..13001)
                     /locus_tag="OUB02_002369"
     CDS             complement(12435..13001)
                     /locus_tag="OUB02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCT8886123.1"
                     /translation="MMNNELNTIILETLSNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13162..13815
                     /locus_tag="OUB02_002370"
     CDS             13162..13815
                     /locus_tag="OUB02_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCT8886124.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
BASE COUNT         5465 a         1349 c         2368 g         4721 t
ORIGIN      
        1 taaactaata ttattataat ttattacata agtaaataaa tctttagaaa ttgtaaaaat
       61 tttatataaa aattcaaata attaatgaat attgaaaatt ttagatatat aataaaataa
      121 gagtatattt tatacaatat gaaagctggt attgcaatag attaaagcct tatacacgtt
      181 tagtttttaa agaaattagt gttattatga aaacctaagt taatttatta aataaataat
      241 tgaataaaaa aattttatta tgtatttata aaatttttgt tacaaatttg gcataatata
      301 gtatacttta agtgtgtatt aggttaaatc tataacaaat tagacgtatg atatagactt
      361 agtgtttatt ggcatttaca agaaatttgt taatatcaac tatttgtagt ttgggcagtt
      421 ataattatta taggggggat gtccaataca aaacaatcaa ccaagaacta atattatatt
      481 ataaagagtt atagttttgt gtgtttatgt ttttaattaa atttaaagtg ggaaaaatat
      541 atttgttaga atggagaata gcgagatgag taataaaata gttagtaaga gacaattaac
      601 agatagtatt tatttaatgg aaatagaggc acctagagtt gcaaaatctt ctcaaccagg
      661 tcaatttata ataattaaaa atgatgaaaa aggagagagg attcctctta caatagctga
      721 ctatgataga gaaaaaggaa cagtaactat tgtattccaa acagttggtg cttcaactaa
      781 aaagttagca atgtttgaag aaaacgattt tgtaatggat tttgtagggc cattaggaca
      841 agcaagtgaa tttatacatg aagatataga agaattaaga aataaaaaga tattatttgt
      901 agctggtgga gttggttctg ctccagttta tccacaagtt aaatggttta aagagcatgg
      961 attagatgta gatgtaataa ttggtgcaag aactaaagaa ttaattatat tagaagatga
     1021 tatgaaaaaa gtagctaaaa atgtatatgt atcaacagat gatggtacat atggatttaa
     1081 tggaagagtt acagacctat taaaagattt agttgataat caaggtaaaa aatacgacca
     1141 agcaatagta attggaccaa tgataatgat gaaatttatg tgccaattaa ctaaagaact
     1201 aaatatacct actatagtaa gtttaaatac aataatgata gatggaacag gtatgtgtgg
     1261 tggctgtaga gttagtgttg gaaatgaaac taagtttgca tgtgtagatg gtcctgaatt
     1321 tgatggtcat ttagttgatt ttgatcaagc aatgagaaga cagtctatgt acaagactca
     1381 agagggaaga gctatgctaa aacttgaaga aggcgatagt catcatcata gtaattgtgg
     1441 atgtggaggt aacaaataat ggatgctaaa aaagtaaaag taccagttag agaacaagaa
     1501 cctgctgtga gagcaacaaa ttttgatgag gtatgcttag ggtataataa agaagaagct
     1561 atggcagaag ctaatagatg tttggcttgt aaaaaaccaa aatgtgtggg tggatgtcca
     1621 gtaggaatcg acataccagg gtttataaca aaaattaaag aagatgatat agaaggtgct
     1681 gctaaagtaa tagctaagag cagttcatta ccagctgttt gtggtagagt atgcccacaa
     1741 gaaagtcaat gtgaaggtgt atgtatactt ggaataaaat cagatgctgt atcaataggt
     1801 aaattagaaa gatttgtagc agattggtca aaagaaaatg acataaattt atctgataca
     1861 gagcctaaga aaaatcaaaa agtagcagta ataggaagtg gtccagcagg tctagcttgt
     1921 gctggtgatt tagctaaaaa aggctatgat gtaacaatct ttgaagcaat gcatgagcca
     1981 ggtggagttt taacttacgg aatacctgag tttagacttc caaaacaagc tgttgttcaa
     2041 cctgaaatag acaatataag aaaattaggt gtaaaaatag aaactaatgt aattgttggt
     2101 aaaacaatta cagtagatga acttatagaa gatgaaggat ttgaagctat attcataggt
     2161 tcaggagctg gtcttccaat gtttatgaat ataccagggg aaaatgcaaa tggagtattt
     2221 tctgcaaatg agtttttaac tagagtaaac ttaatgaagg cttatagaga tgattatgat
     2281 acacctatta gttctggtaa aaaagttgct gtagtaggtg gtggaaatgt tgctatggac
     2341 gctgctagaa ctgcattaag attaggttca gaatcatata tagtttatag aagaagtgaa
     2401 aaagagcttc cagcaagagc tgaagaagtt catcatgcaa aagaagaagg cattatattt
     2461 aatacattaa caaatcctaa ggaaatttta gtagatgaaa atggatatgt taaaggtatg
     2521 gtttgtataa gaatggaatt aggagagcca gatgattctg gaagaagaag acctatagaa
     2581 atagaaggtt ctgaatttgt acttgatgta gacacagtta taatgtcact tggaacaagc
     2641 ccaaatccac taatatcttc tacaacaaag agtcttgata taaataagaa gagatgctta
     2701 ataacagatg aaaatggtca gacttctaaa gagggtgtat ttgcaggtgg agatgctgtt
     2761 actggagcag caacagtaat atctgcaatg ggtgctggta aaacagcagc agcatcaata
     2821 gatgaatatt taaaagcaaa agttaatgct taatttgtaa attaaagaga gggtgtctca
     2881 aaatgaactt tttagttcat gaggcactct ttttgtacaa aatcaaatat gttttaatca
     2941 tttcaacaat gaaaatttca ttcctgtaga aagtctaact tttaggtctt agtacaatct
     3001 ctattttgat gtagccactt ttttagatgg tttaataatt tatcagttat tattgtaata
     3061 tttgatacta agtaacataa ctttgtctaa ctatgataaa atagttatac aataaaggtc
     3121 ttgtaaggag tgaataatgt gtggctaata aaatcagatt tgtcaaatct aacattaaat
     3181 aatagaaaaa taaattgtgt aattaatggt attttaatat gaaaaaaaga gaaaaagtat
     3241 ttgatgggct agtttttaag catatattaa tcttattaat actattagtt ttgattgatt
     3301 taacaagtac catcatatat gcaagtaact ctatggaaat tacttctaga aatatgattg
     3361 aggcatcaaa aagagaactt gaaaactatt tagaagtaaa catatcttta ttaaaagctc
     3421 ttagtcaaga tgatagattt tctaatggtg aaacatcact aattgaaaag ggaaaattat
     3481 taagtccata tcaaaaggaa tataatcttt ttatgattgg tataacagat acaaaaggaa
     3541 atgcttcaag tacatatagg gaaaatgtta actcaataaa agataagcct agttttgaga
     3601 aagcaattaa aacaaagcaa gtagtagttt cagatattga agtcagtaat gtaacaggtg
     3661 acaaagtatt tatcatatat gtaccaattg taaaaaataa tgagatgatt gggaccattt
     3721 ttgcttcttt ttattttcaa gatgtaaata atttaataag tagaagcaat tttgatgatt
     3781 ctattaaatt tctaatgata gataaagatt atactattat ttctcatcca aacaaaaaat
     3841 atgtaaatga taaaagtaag atgcttgatt tagaagggaa tattatagga accactaaaa
     3901 gtgaaatctt aaaaaatata aataaaaaat gtcaaggtga ttttttatct tgggataatt
     3961 ggagattata taatgtaaaa tatacgaaca taaagtggac taattggact ttagtatcaa
     4021 aatgtaatat atttaagaac tttaaaaatc taatagttaa ttttatgata aagttgtatt
     4081 tttatatagt aatatttatg atattgtgga aattaagtaa tgctaaatta atagaacaac
     4141 ttaaaaaatt ggcttattat gattctttaa gtggaataaa aaataaagaa aagtttagaa
     4201 aagattctat gtatatttta aagaattatt atcaagataa tttttactta gtacaattgg
     4261 atgtcaataa atttaaatat ataaatgaga tgtttggata tgctgaaggg aataagatat
     4321 taattcatat atcacaagtt ttaaataata atacaaataa atatgaaatt tgtgctagga
     4381 tggataatga ccattttata ttgttgattg cttgtaacac tgaagatgaa cttttaaata
     4441 gattatctaa aataaataaa gaaatttgta acttaaatac tactaactca tctaagtata
     4501 aaattgttat gtcaagtgga atatataaga ttactaaaaa ggatgatatt aaaaagattg
     4561 acttgttaat tgatagagca aatattgcag caaaaagtaa aaaggaaaaa tatgaacatt
     4621 catattcatt ttttaatgaa gatacaagaa atcgattgta caaggaaaaa cgattggaag
     4681 ataatatgaa taaagcttta gaaaaaggtg aatttatagt atattatcaa cctaagtata
     4741 gtttagatga tgtaaatgaa attgaaggag cagaagcctt gataagatgg aatagtccag
     4801 aatttgggtt tatttcacct atagattttg tacctttatt tgaaaagaat ggttttattg
     4861 taaatattga tatgttcgtg tttgaagaag tatgcaaaac attaaacaag tggataaata
     4921 aagggtatac tcctgtacca atctctgtaa atatgtctag agttcattta tatagggata
     4981 attttataga aaatataact gatttgataa gtaaatacaa tatatctcct gagtttattg
     5041 agcttgaact aacagaaagt gttgtgtttg ataatttaaa tatattgatt gatataatga
     5101 aaaagattaa agagatagga ttccttatat caatggatga ttttggttcg ggatattcat
     5161 ctttaaatct gttaaaagac ctttcttttg atattttaaa attagataga ggatttttaa
     5221 tcgaaacaac agatacaaaa cgtggaaaaa ttattatttc taagattgtg gaaatggcaa
     5281 aagcgattga tattaaagta atctgtgagg gcgtagaaac ttatgaacaa gtagagttct
     5341 tgaaagagat aggttgtgat aaggttcaag gatacttatt tgctaaacct atggtattag
     5401 atgaatttga aaaacactta aactttaaat ttgattaatc attaataatt attagactat
     5461 aagataccct aacaacttac ttacaaatat gtaagttaat tgttagggta tttttatggt
     5521 taaagtagta tattcacgat attatggtat tgtagtaaaa ataaaaaatt atggagaata
     5581 taaaatgaac atattgagta ttagaaatat atcaaaaaca tattgcggaa atattccatt
     5641 taaagcatta gataaggtta gtctaaatat agaaaaggga gaatttgtgt ctgttatggg
     5701 accatctgga agtggtaaat ctactctttt aaatataata tctacagttg atagacagag
     5761 tgaaggtgag gttgtattag atggatatga tgtaagtaaa ttgaaaggag aaaaacttgc
     5821 tgaatttaga aggaaacaac ttggttttgt atttcaggat tttaatttga ttgacacttt
     5881 aactgttggc gaaaatatta tgttgccact tacactagag ggggaaagta tcaaagatat
     5941 gaatatacaa actaaaagta tatctaaatt tttaggaata gataaaattt tagatagaaa
     6001 aacctatgaa atttcaggag gtcaagctca aaggtgtgct attgctagag ctattataaa
     6061 taaacctgcc atacttcttg cagatgagcc aactggaaat cttgattcaa aatctactga
     6121 tgatgttttg aaattattta ctaggataaa caaagaacaa aaggtaacaa cacttatggt
     6181 aactcatgaa gcatatagtg caagttattc agatagagta atttttataa aagatggttg
     6241 tatatataca gaaataaaaa aatctgaaag cagtaattct ttctattctg atatactagc
     6301 tgttttgtct caaataggag gtgtaagata gtggatttta gaaagtttgc ttataataat
     6361 atagttagaa atttcagggc atatttagca tattttttaa gttctgtttt ttctataatg
     6421 atattttttg tttttgcagt gtctatgttc catccaatta taacaaaatc tggaatgcag
     6481 agtggttcta ctgcatttat ggctcttatg acttcagaat taataatatt cggttttagt
     6541 ctacttttta tattgtactc tttaggaaat ttcttaaaat atagattgaa agattttgga
     6601 gtattgatga taattggaat gagtaacaga caactgaaaa aattgatatt aattgaaaat
     6661 ttgataatag gatttttagc agtgatgtta ggaatattac ttggtttatc aatttcaaaa
     6721 ttgtttttat tgtatttaag caaactgttt tatatgaatt taactgaatt ttattttcca
     6781 gtaaaagcaa tgctattgac tattgtatca tttatgattt tatttttgat aacaggacca
     6841 atgagcttaa aattattgag taaaaagagc attttagagc ttttagtagg tacaaaaaag
     6901 cctaaaaaag agataaaatc ttcaaagcta tttggaataa ttggtgtaat atgtcttata
     6961 ttaggatatg ctatgatact atttggcaat aagttaaaaa taaatggagg tatgtataca
     7021 acagcactat taattacttt ggggatattt ttattttttt ctcaatttac tattttgata
     7081 ttaaagtatt taaaaaataa agaaaaattt tataagaaca aagtaaatat gttacttata
     7141 agtaatttga tatataaatt aaaggataat acacgattgt tatttttaat aacaatactt
     7201 ttatcggcta ctttagtagc atttacaaca acttcaacac ttgtaagttc tcaaggagaa
     7261 gcttctaaaa ataatttccc aatggtttac tcatattttt tagagcataa taatgaaaaa
     7321 gaatatgaag aactacaaca aattagagaa gcaatcaaag gatatgatta taaggagtta
     7381 agttttcctg tacttaaata taatagggaa gtcttactaa gtgtaaagaa ttataacaaa
     7441 ttatcaaagg aattgggatt agataaaatt agttcaaagt tagaaaaata tgaagggatt
     7501 ataattccta gtagcaattc gcaagaacat ataaattctt taaaacaatt aaaaaattat
     7561 agtataaaaa atttaaatat agaagtaaaa aaatcaaaaa ataaaataat ttttcctaca
     7621 ggactgttta gtaaagttgt agttataagt gatgacttat acaatgaagt aaaaggtgaa
     7681 ttttcaaaaa ttaattttca tggatttgat tataaaaact ggcaaagcag tgcacagata
     7741 actgaaaatt taaaagaaaa acattttaat ttggatagag aatataacag gtcatatttt
     7801 ttaacattac cagatatgta tttagtagag ttacaacaaa gtaaaattct tcaatttatg
     7861 ggtgttttta taggagtgat attatttata gcagttttta gttttttata ctttagatta
     7921 tatacagacg agatagaaga taatgtaaag tacaaaaaac tatcaaaaat aggtctatct
     7981 tttaaaaata tgaataagat tgttagtata gagataggta cattgttttt tataccgtat
     8041 tttatagcaa ttataaatag cataatttct ttactgtttt taaatcaagt attaaataat
     8101 agttttaagt tagaaaattt gggtatatta ctactgatat catctatata ttcactttat
     8161 ttttacttat taaaaaaaat ttatactaga aaagtttggt tttttgatta aagtggtata
     8221 taatagatat ataaattaat atttaaaaag tagttaatat taggggaggg catttgcacc
     8281 tcccttgtaa tatgtggaaa attgaatatt agaagaagga ggagacttga tggaaaatca
     8341 acaattatta ggaacagaac gaattagtaa attactacta aaatattcta taccagctat
     8401 aataggtatg ttggtaaata gtttatataa tgtagtagat agaattttta ttggaaatat
     8461 accaggggtt ggaccacttg caataacagg tcttggtgta acaatgccaa taatgacaat
     8521 aatacttgca tttgggatgt tgataggtat aggaactact actaccatat caataaaact
     8581 tgggcaaggt aaagtagaag aggcaagaaa acttatagga aatgcaatga cgttatctgt
     8641 aataacagga attatcataa tgatacttgg aatattattt gcaaataaaa tacttacatt
     8701 gtttggagca agtgaaaata ctttaatata tgcaaaatca tatataaata ttatattatt
     8761 aggtacagta gtaaatcttc tttccttttc tttaaaccac tcaataagag ctgatggtag
     8821 tcctaaaatt tcagcaggaa taatgatagt aggatgttta actaatatag ttttagactg
     8881 gatattaata tttggattca atttgggaat acaaggagct gcaattgcta cagtaacttc
     8941 tcaggctctt acagctattt taactatagg gtattatata agtggaaaat caaacttacg
     9001 attcagtaaa tctaatctaa aattagataa aaagctaata aaagcagtgt ttgcaatagg
     9061 tatgtcgcca tttgctatgc aacttgcagc aagtttagtt caagtaatat caaatattgc
     9121 acttaaaact catggtggag atttagctat aggggctatg gctactattt cttctattgc
     9181 aatggtattt ttgatgccta tctttggaat aaatcaagga gcgcaaccta ttataggttt
     9241 caattatgga gctgaaaaat atgatagggt taaaaaagca tatttaggtt cattagtagt
     9301 agctactata attttatgta tgggtatggt agtagttatg cttttcccag aagctattat
     9361 aggtatattt aataaagacc ctgaacttat gaatatatct gttaatggat taagaatata
     9421 cttacttatg ttgccaattg taggcttatc agttacagga acaaacttta tacaatctat
     9481 aggaaaagca aaaatggcta tgttattgag ccttttaaga caagtaattt tattgatacc
     9541 agcagtatta attcttccta cattcttagg cttacagggt gtatggacag ctcaaccagt
     9601 gtctgatttt atagctacag taattacagg tatagttgta tttagagaat taaaaagata
     9661 tactcctaaa actgaaaaat taaatgaaaa tgaaagacta aacgagataa ctactgagta
     9721 aaaatataaa ttcagataat tgagcaagaa attgcaaaaa atacgattat atgataaaat
     9781 aaaaccgctg tcaaggataa gagaaattct aattatggag tgattttatg gaatttacaa
     9841 agaaaagcaa attaaaaaat atgtatgtaa acatgaatgt agctagaatg ttgtctaaga
     9901 taaatgagta taagggtaga caactgcttt acaaaaaaca accaaaagaa attttagaaa
     9961 acttagaaaa aaaatcttta gtagattgtt ctgaatctac atacattgga aatcaaagag
    10021 atagtagtaa ttttaatcta gaaaagttga taagtaatga agttacacct agaagtagag
    10081 aggaattgag tattgttgaa tacagagatg tggtaaaaac tattaacagt gcttatgaga
    10141 gtattcccat cagttcacaa acgattcttg agcttcatgg atacttatat aaattttcat
    10201 ctactagagg aggaagttat aagtcagata atgattttat tgaacacaat ttaaaaccta
    10261 gcgaatttat aagtactgat tcaagtgtca ataaagttga aaaagctata gaagaaatat
    10321 gtgaagctta taatacattg atagaggaag atgaaattga tatattaata ttaatttctg
    10381 catttgtgtt agatttcata ttgatacatc cattcaaaga aggtaatata aaaatggcga
    10441 gagtacttat cctattgtta ctaaataaaa atgggtatga agttggaaga tatataagtt
    10501 taggaaagat atttgatgat agttcatatg aatattatag taatttaaat tatttgaaag
    10561 cctcaatagg tagtgaaaaa gcagatatga atgcatggat tgagtatttt ttagagacga
    10621 tattaactgc atatgagaag ctggatgata gtttaaatat atctgataag aaaagacaaa
    10681 caaaaactag tagaatagag aaaataataa attctaccct tggatatttt acaaaagaag
    10741 atataagaga tttatgtcct gacattccag aacctactat aaatagagtt tttaataatc
    10801 taagaaaaca agataagata gaagttgttg caagaggaag aagtgcaaaa tggaagaaaa
    10861 aatattaaaa aatgtttaga taactaaaat ggtatagtgt tttacactat accattttta
    10921 atgtcattag catgagcaat aatttaacaa cttaagtaat atcaatttta tttattgatt
    10981 atttttgaac tgaaaaatgg cgttatatgg tatacctcag tggtaattaa ttttggatga
    11041 gaagatataa tattacagaa tatattagga gtatatgtag aaaaaatata ataaaagtgt
    11101 gaggcttagt aattaacaaa ttatgtggct tcacactttt ttataaaata aagatactta
    11161 gcagaataat attttagttg atatgtttat aagatggtac ttgtttgggt aaaagtataa
    11221 atagtaaaaa agtagaagta atgtgtataa aaacataagt tacattctgt aataatagtt
    11281 attttataat tcaacaaaag aatagaatgg gtatatttgt gaaataataa tataaaataa
    11341 tcttaggagt gagcttatga agaaaaaatt attatatata aatgtaaatt caaaacctga
    11401 agacttatct tcaagtaaaa ctgtagcaag aaaatttata agtaaattta tggaaaaaaa
    11461 taaagatttt gaggttgaag agatagacct ttataaagaa catataccaa gacttgagta
    11521 tcagtacttt gaaaaaagaa atagcatagt tagtgaagaa aatgctaaaa atttagacga
    11581 taaggataga aaagaactta caaagattag aaatctttgt gaccaatttg tgagtgcaag
    11641 tgtatatgta atagcagcac ctatgtggag tttatctttt ccagcaccac ttaaagagta
    11701 tatagattgt ataattcaag atggaaaaac tatatctttt gaaggcaatg ataaaccaca
    11761 aggaatttta aatgatattg atagaagtat ggtatatata caatcttctg gtggacacat
    11821 accatgggtg ttgaagccag ttatgaataa agggttaaat tatgtggaaa gtattatgaa
    11881 gtttatagga attaaaaaat ttgatgaatt gttagtagat ggaactggaa ctagtgaaga
    11941 agaaagacaa gctgcaatag aaaaagcatc tgaaaaaata gatggtataa tagatgggat
    12001 gaaattttaa ttataatatt ttattaaaaa tataaaggaa aaacctcttt taatgtagaa
    12061 acttatattg aaggaggcga gaatcatgcg agaagaaaaa agtaatgaaa agtatgattg
    12121 ttattggtgt aatcaagaga ataatttctg tgtagaaata aaagataata tagtcatgat
    12181 agatgatggc actggtacgt taaaacaagc cgttttcata gggtataaac aaatccaaat
    12241 taatttaaat tgttcacatt gtcaaaactt aaatagaata aaattaaatt tgtagaattt
    12301 taaaatggaa attattgaag gaattaaaaa tagaaagtgt taatatttag actgaaaatt
    12361 atattttata caataaaagt ttatttaaat aacattgtat tttaatattt attttcagtc
    12421 ttactttatt aaaattattt tttggttatg tctggaaaat atctgtctac taatttttct
    12481 gaaatatttt ttaattggtc agccattgaa gtcagactta ataaggctat caataaatca
    12541 tctttatttt ctggatttat actaatattt tcaaaattag actctataaa gtccggtata
    12601 ttctttcttt gattttcttt tattaacatg aatttttcgt aacatgaaac taaatctttt
    12661 tcactgaaat tatccttttc atacacacca tgtaagattc ttttaatttc ttgtattgta
    12721 agtgtatttt tcatagaata aataattatc atctgtaaaa tttgttcttt agtatacttt
    12781 tttcccttaa ctggtttgat taatccctct ttgctgtaat tatttatcat ggttttagta
    12841 agaattttat cactttcaaa tcttttatta gcagaaagct tattgtcaat tagagaaata
    12901 atttgatcca tatataagtc aatagatgga atatcgtttg aagttatatc tgcattactt
    12961 agtgtttcta gtattatagt attaagttca ttattcatca tatgttacct ccataatata
    13021 tataatacac taaattagaa agtaaaacaa tattaaggct tgtaaaaaaa acataaagtg
    13081 gtataattaa atatataata tagtaatgga aactatattt gtgaatctga tttttaaaat
    13141 acataattgt aggaggacga aatgtatcaa ttttttttaa agggtagaga cccaataagc
    13201 agtttaactc attttatagg tgcttgttta tctcttttag caactataat tttagttttt
    13261 caatctgtta cattacagga aacttctctc ttgatgatag tatctgtatc tgtatttgga
    13321 ctttcactaa ttgctttata tagtgcaagt tcgtattatc attttttaaa ggggacacca
    13381 gaacaagaat tgttttttag aaaagtagac catgcaatga tttacgtatt gattgctggc
    13441 tcttatacac caatatgtct gaattttatg gaaaaaaaag aaggcataat atttgttaca
    13501 gcaatatgga tagttgcatt tattggcata ataataaaaa tattttggat ggatgcacct
    13561 agatggttat ctaccagtat atatttactt atggggtggg caattgtttt tgatataaat
    13621 gcattcaatt caattcctaa ggattgcctt agactattaa ttatggaagg tgtatcttac
    13681 tctataggtg cgattattta tataattaaa aagcctaata taagtcctga gtttggattt
    13741 catgaaatat ttcatatttt tattatgata ggttctttat ttcacttttt agcagtttta
    13801 ttgtatgttt tatagtttta aaagatttga ttaattgata ctaaaattct acaatgaata
    13861 aaatggaact gccttatata gattatatct tagacaataa gac
//
LOCUS       DAKBNN010000099        13774 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000099 DAKBNN010000000
VERSION     DAKBNN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.099"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            160..300
                     /locus_tag="OUB02_002371"
     CDS             160..300
                     /locus_tag="OUB02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886125.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(475..795)
                     /locus_tag="OUB02_002372"
     CDS             complement(475..795)
                     /locus_tag="OUB02_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCT8886126.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(915..1652)
                     /locus_tag="OUB02_002373"
     CDS             complement(915..1652)
                     /locus_tag="OUB02_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCT8886127.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1867..3690
                     /gene="acd"
                     /locus_tag="OUB02_002374"
     CDS             1867..3690
                     /gene="acd"
                     /locus_tag="OUB02_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCT8886128.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3905..8203
                     /locus_tag="OUB02_002375"
     CDS             3905..8203
                     /locus_tag="OUB02_002375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCT8886129.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8407..8868
                     /locus_tag="OUB02_002376"
     CDS             8407..8868
                     /locus_tag="OUB02_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCT8886130.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8898..10052
                     /gene="nusA"
                     /locus_tag="OUB02_002377"
     CDS             8898..10052
                     /gene="nusA"
                     /locus_tag="OUB02_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCT8886131.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHLGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10059..10340
                     /locus_tag="OUB02_002378"
     CDS             10059..10340
                     /locus_tag="OUB02_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCT8886132.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10330..10641
                     /locus_tag="OUB02_002379"
     CDS             10330..10641
                     /locus_tag="OUB02_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCT8886133.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10663..12603
                     /gene="infB"
                     /locus_tag="OUB02_002380"
     CDS             10663..12603
                     /gene="infB"
                     /locus_tag="OUB02_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCT8886134.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12646..13026
                     /gene="rbfA"
                     /locus_tag="OUB02_002381"
     CDS             12646..13026
                     /gene="rbfA"
                     /locus_tag="OUB02_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCT8886135.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13007..13684
                     /locus_tag="OUB02_002382"
                     /pseudo
     CDS             13007..13684
                     /locus_tag="OUB02_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5500 a         1558 c         2585 g         4131 t
ORIGIN      
        1 tttaataggt aaaatatcct attgttttgt agatatatcc taattatgtc aaaaaataaa
       61 aaaattttta tttattagag tttttggaaa taataataac agatattata attaattaac
      121 agtttttgta agtatttgac aatagagagg gtgtaacaaa tgggaataat agagtcagca
      181 tcaaaattgg ctgagatggt tcatttattg gctgttgaaa aggggataac tgatatagaa
      241 gcatgggatg aagctgtgaa agaatacagc aaaatttatg aagaaagaag aaatgaatag
      301 aatgataaaa aatgaaaact tgatgataaa aaataacttt atttaaagaa attcaatttt
      361 tatagaagta ttaaaaataa tataaataaa aagatggtat ttcaaatgta agtaaaaaat
      421 cattttggga taccatcctt attatttatt ttaataatat ataagtaata aatcttactt
      481 atccatattt tttatgtaac ctcttttttc tagctttttt atatgctcct tacgtatttc
      541 atcaagatta ataccatatt gttcttctag cttaaacatc atagtaacac atgtctgcgc
      601 tacatccatt agttccttag cagttttctt tataatatca acttcatcta aatcattatt
      661 ttctccactt agatttctga atttacctat acattcagct aattcacctt gttcttcacc
      721 cattttgata aatgtagatt ctagtgttgg gtctaaatta ttaagctctg gtaaagatat
      781 agtttttagt ttcataaaag ctcctttcaa gttattcgta tttatactaa aacatcataa
      841 caaaaataat actaaaaaaa aactttaaat tcaacataaa aacttacata tataataaaa
      901 atcgtgaaat aaatctatat ttgtcttaat acagcctcat ataaaacttc tccaataggt
      961 ttatttatta caagagaagc tgacttatca tagactgtat ttgctttatt aataagagca
     1021 atataatcac ctttgtaata atctataaaa cttgctgctg ggtacacagc taatgatgta
     1081 cctcctataa tcaataaatc agcattactt atagcagaaa ttgttttagt aatgacatca
     1141 ctgtcaagag cttcctcata aagtactaca tctggtttta ctatagaacc acagttgtca
     1201 cagtaaggaa tattacctcc cagatttaac atagattcta agtcaaaaaa cttaccacat
     1261 ttagtacaat aatttctatg tacagaacca tgaagttcta gcacattttt acttccagcc
     1321 atttgatgaa gtccatcaat attttgagtt atgacagctt taagcttacc catttcttcg
     1381 agttttgcta aagctatatg agcattattg ggttttgcat taggatatat taatttatcc
     1441 ttataaaatt caaaaaattc ttctggatat ctaacaaaaa aagtatgtga aactaattgt
     1501 tcagcagtaa attgtttatt gagtttttgg ctaaataatc cagtagaact tctaaagtct
     1561 ggaatattag attcagttga aacacctgct ccaccaaaaa atacaatatt attgtgatta
     1621 gctattaagt cttttaagct atttgcatcc atagttacac ctccaaatta ttatatataa
     1681 attgtactac atattaggtc gtttatgttt tattaattta aataaatata tagtttaata
     1741 agttaaataa agggaatata aataaaatgt atacagagta taaatagaca atatttacta
     1801 aaataacaag ttgtcgtata attatattaa aattgtttta gtgtataaga agggcggggg
     1861 aagtacgtga agaaaaaagc agctttagca acattggcta tgttaccctt aggtgtggta
     1921 aatgcacatg ctgatggaga tataggtata gtgactataa attatttaaa tgtaagaaat
     1981 gaaccaactg ccgaaagtag catagccttt gttgctaaaa aagatgataa agttttgatt
     2041 aaagactctt ctaatggatg gtataaaata aaagctgaat ctggacaaga aggttgggct
     2101 tcatcaaaat atatagcaaa atcaaatagt gactctttaa gaacgtctac aaataaagaa
     2161 aaacaagtta tttcaaatag tttaaatatg agaaatggag caggtacaag ttacagagta
     2221 ataactgtac taaaaaaagg tcaaaaagta gaagtaatat cagagagtaa tggatggtct
     2281 aaaatcaaat atgatggaag actaggatat gtatctagct cttatttagg agacgtttca
     2341 aactcaacta ataaatcaaa aactaaacaa gtgaatacaa cttcactaaa tgtaaggagt
     2401 ggaccaaata caagttatgg tttgttaggc aagttgccaa aaggaagtaa agtagaagta
     2461 atatcagaaa gtaatggatg gtcaaaaata aagtataatg gaaaagatgc ctatgtatct
     2521 agtatgtact tatcggatgt aagtcaaagt aattcagaca attctagtca aagcaatgat
     2581 aaaaagaata ctgataaggt tgtaaataca gcttctttaa atgtaagaag tggaccaggt
     2641 tctacatata gtaagttggg aaaagtttat aaaggaagta aagtaactgt actatcagaa
     2701 agtagtggat gggctaagat taattttaac aataaagaag catttgtagt aggcaattat
     2761 ttatctactt cagcagatac ttcaaataat aactcaaata gtaactctga taacagttct
     2821 aatagtaatg gcaataattc ttcatcatca ggtcaagtaa atggtatgtc aggtataagt
     2881 ggggctaaaa ttgattataa atctttgagt tatactttag aatctcatat aagtaaacaa
     2941 gttgagaaag cagcatcagg aggaaatgtg atagctccaa gtaataggaa aagcactcca
     3001 agtcctgaat ttagtacttt ttcagctcaa agaacaagct catttgtaaa tgcaagttca
     3061 agtgatatag aatattattt aaatcctaag aattttacaa atactactaa aggtatgatg
     3121 cagtttttaa agattaacag ttatagagat ggtatttcag aatcaagtct taactcgtat
     3181 cttaatggtt tatcttctag tgtatttaaa aaccagggag cagcctttat aaatgctgcc
     3241 aagaagtata atatagatgt tgtatatcta gtatctcatg ctatgtggga aactgcttat
     3301 ggtaagtcta cacttgccca aggtcaaaca ttaacatctt ataagggaca acctcttagt
     3361 aagccagtta aagtatacaa tttttttggt ataggagcaa tagataaaag tgctaatgtt
     3421 tcaggtgcag aagcagcgta ttccaatggc tggactagtg tagaagctac aatagatggc
     3481 tctgcaaagt ggatatcaca aaattacgta aatagttcta agtacaatca gaatactatt
     3541 tacaagatga agtggaatta tgattataca tggcatcagt atgcaactga tgtaaactgg
     3601 gctaatggaa tttctggaat tatggaaaat ttaattggtc tttatggagg aggaagtagt
     3661 ttggtttttg aagttcctca atataagtaa tacataaata aaaaatatag actatgggta
     3721 gttttcattt tatttaaatt atctatagtc tttttgtata taatttataa tctgtaaatt
     3781 taatgttaac aaaactttta tgttataata ttaattctaa gtaaatataa gtgagtaatg
     3841 ggtagaaata gataaaaagt ggtaataata tatataaaga taattttgag aaaggaggat
     3901 atatatggaa agtattaagg aatatttgga taagttagaa attaataata gtggtcttgg
     3961 aaagcaactt aaagaagttt atataaatag agtagtttat tttaaggaag ataagatagt
     4021 ttatttttat ctaacttcta aagatattgt ttctcatgaa cttttagata aatttaaaga
     4081 agaattgatg tataaactcg attattttaa agatatgagg atgaagataa gatttactgg
     4141 tcttgaaaga aaatccaata aagatgttat aaaaaaatat tggaataata tattgtatat
     4201 attaaagtat ctttgtccat ctattgcagg gtggtataaa caagtagaat ttctttgttt
     4261 agaagaagaa ttaaaaataa aacttccaaa aggtatattt tatgagcgat taatgaaaaa
     4321 aaatgtaact tatgtactta aaaccgtgct tagcgaagag ttgggattag atttaaatat
     4381 aacaatagaa aaagctgttg atgaaaaggt aaataaagaa agactcataa gaatcaatga
     4441 tagagaaatg gaagaaaaaa ttagggcttt agaaattgga aaagttaata actgtgaaga
     4501 aaatgaagag gaaagttatg tcattaagtc tgatgtagat gaaaatttaa tctatggaga
     4561 taatgcaaat gctatgattg aaaatatagt tgaattaaat gcatcttctg gaacagttgc
     4621 agttgttgga gatatatttg atgttgacac taaagaactt aaaaacggaa aaatacttat
     4681 gatagcttca attacagatt atactagctc aataagctgt aaactttttt taacagatac
     4741 aaataaggat ggagtacttg aaaatgttaa aaaaggagca tacttaaaaa tcaagggtga
     4801 catagtttat gatacatatc aaagagaaat tagtatgatg attagtggga taagaaaaga
     4861 aactagaata gagagagaag ataaatcaga ggaaaaaaga gtagaactcc atgcacatac
     4921 acaaatgtct tctatggatg ctatttgttc tgttaaaaag cttgtagagc gtgcagccaa
     4981 atggggtcat tctgctattg ctataacaga ccatggtgtt gtacaaggat ttccagatgc
     5041 tatgaatgca ggtaagtcaa ataatataaa aatactttat ggagtagaag ggtacttggt
     5101 tgaagatgat tctctcatta tagaggatgc aaatgataaa gaactttcgc agacatttgt
     5161 tgtatttgac atagagacaa ctggcttttc taatacaaat gataaaataa ctgagattgg
     5221 tgctgtaaaa atagaaaact ttaagattgt agatagattt agtgagttaa taaatcctga
     5281 aaaagatatt tcttataaga tacaggaatt gacaggaata actaatgaat taattaaaga
     5341 caagccaacc attgaagaag ttcttcctaa gtttatggaa tttgttggag atagtgtatt
     5401 agtagcacat aatgcagagt ttgatactgg atttatatcg caaaaatgta gggaacaagg
     5461 cttgttatat aataacaaaa aagtagatac acttatgtta gcaagagtaa tgttaccaaa
     5521 cttaaaaaga catagattaa atgtcgttgc aaaagaactt ggtattcctc ttttaaatca
     5581 ccatagagca gtggatgatg ctgaagctac agcacttata tttaataagt ttttgcagat
     5641 gcttacagaa aaaggtgcaa aaactctaag tgatgtaaac aatatacttg gaaagattga
     5701 ttacacaaag cttagcacaa atcatataac cttgatagca aaaaattcta taggtataaa
     5761 aaatttatat aaaataattt cagatgcaca tgtaaatcat ttttttagag caccaagaat
     5821 ccttagaagt gtattaaatg aatataaaga aggattaata attggttcgg cttgtgaagc
     5881 tggggtggtt tttcaagctg taaagaaaaa cgtgtctgat gaggaaatga aaaaaataat
     5941 agatttatac gattatattg aagtaatgcc tatagataat aatagattta tgattgataa
     6001 aggagaagta aaggacgaag aagagttaag ggagttgaat aggaaattaa ttgatacagc
     6061 caaaaaattt gacaaaattc cagtagctac tggggatgtt cattttttag acaaacatga
     6121 agctgtctta agaaaagttc ttaaatattc tcaaggtttt aaagtagatg aagaagaaac
     6181 ttatttacat tttagaacaa cagatgaaat gttggaagag tttagttatc ttggagaaga
     6241 attggcatat gaagttgtag ttgaaaatag taatctgatt gccgatatgg ttgaagatat
     6301 aaaacctata ccagatgata cttatcctcc aattatagag ggctcagatg tggagttaag
     6361 agaaatgtgt tacaaaaaag ccaaacgaat ctatggagac ccaataccag aaatagttca
     6421 aaaaagactg gatagagaat taaattcaat aataagcaat gggtatgcag ttatgtatat
     6481 tatagctcaa aaactggttg caaaatctct ttcagatgga tatttagttg gttcaagagg
     6541 ctcagttggc tcatcttttg cagctactat gagtgatata acagaagtta atcctcttcc
     6601 agcacattat atttgtgaaa acgaagactg taaatattca ttcttttatg aaataggtga
     6661 atggggctca ggtgttgatt taccagacaa ggattgtcca aagtgtggaa gaaaacttgc
     6721 taaaaacggt catgatattc catttgaggt tttcttaggt tttgaaggtg ataaggaacc
     6781 agatatagac ttaaatttct caggaaatta tcagccaaca attcataaat acacagaaga
     6841 actatttgga gaggggtatg tatatagggc tgggactata ggtactgtag cagaaaaaac
     6901 agcttttgga tacgcaagaa aatatgtgga agaaaataat ataagtgtac caaatgcaga
     6961 agtacttagg ctttcaaatg gatgtactgg agtaaaaaga acctcaggac aacatcctgg
     7021 tggagttatg gttataccag actataaaga tgtatacgat tttacaccta tacagtatcc
     7081 agcaaatgat acttcttgtg gtgttataac tactcacttt gattatcatt caataagtgg
     7141 tagaatactt aaacttgata tacttggaca cgacggtcct accattataa ggatgcttga
     7201 agatatgact ggaataaata taacagaaat tcctttagat gataaagaga ctatgtctct
     7261 atttacatct acagaagcat taggtgttac accagaagaa ataaactgtc ctataggttg
     7321 ccttgctgta ccagagtttg gaactaagtt tgtacgacaa atgttacttg acacaaaacc
     7381 tactacattt gcagaacttg tacgtatatc tggtctctct catgggacag atgtttgggt
     7441 aaataatgca caagacttag ttagagaaga tatagttgga ttaaaagatg ttatatctac
     7501 acgagatgac ataatgaact atttaatatt taaaggatta cctcccaaaa tgagttttac
     7561 aataatggag agcgtaagaa aaggtaaagg tttaaaacca gaacacattg aagaaatgaa
     7621 aaaaaataat gttccagaat ggtatatagg ctcatgtaaa aagataaagt acatgttccc
     7681 aaaagcacat gctgttgctt atgtaatgac atcatttaga attgcatatt gtaaagttca
     7741 ctatccagaa gcattttatg cgacttattt tacgacaaaa gttgaggatt ttgatgcaga
     7801 tttaattgtt aaaggattag atgctataaa atcaaaaata aatgaaattg agtcactggg
     7861 aaatgatgca accacaaaag aaaaaggtat gttgaccgtt ttagaagttg ctcttgaaat
     7921 gtatgctaga ggaataaaat tattaccagt tgatatatat aaatcagatg caactgagtt
     7981 tatagttgta ggagaaaaaa cattacttcc accaatggca gcaatacaag gtcttggagg
     8041 aaatgcggct ataaacatcc aaaatgaaag aaaaaatggt gaatttatat caaaagaaga
     8101 tttgagaaag agaacaaaaa tatcaaaaac ggtaatagaa actcttacta atcatggttc
     8161 acttgagaat atgagtgaga gaaatcaatt gtcattgttt taagttagcc caaaaagttg
     8221 caaataaaag aattttatga tatcatagta tagaaaatag tatactttgc agaaactaag
     8281 agtgagacag ctgttctcac tcttttattt taaatatatt attaaaaagc tggtttattt
     8341 ttattaaata tattttattt aagattatat aaactggtca tttacaattg aataggagga
     8401 actttaatga aaaaaaattt agaagctaca attgaggaga ttgtaactaa aataacggat
     8461 gaacatggat ttgaaatggt tgatgttgaa tatgtaaaag aagcaggaga atactactta
     8521 agagtataca tagataaaga agaaggaata tctttaaatg aatgtgaatt agttagtaga
     8581 gaattaagtc caatactaga tgaaaaagac cctataaaag aaaattactt tttagaggta
     8641 tcttctccag gacttgatag agcattaaaa aaagacagag attttgttag atatcaaggt
     8701 agagatgtag atttaaaatt atataaacca ttaaatggat gtaagcaatt tgaaggtgaa
     8761 cttgtaggat taacagaaga taataatata aaaatcattg caaatggtaa ggaaatggaa
     8821 tttaatagaa aagatgttgc tattgtcaga ttagcaataa agttttagtg gtatattaaa
     8881 tagggaggat ataaaaaatg aatcatgaat ttatggaagc attagatgaa ttagtagttg
     8941 acagaggtat agataaagaa atacttatag atactataga acaagctctt ttaactgcat
     9001 acaagaaaaa ctttggttca gcacaaaatg ttagagttga atttgataga gacaagggag
     9061 atataaaagt attttctcaa agagttgttg ttgatgaatc agatttatat gatactttct
     9121 tagaaataga gttagcagat gctagagaga taaaccctaa ttatgaatta ggtgatatta
     9181 tagaacatga agttacacct aaaaactttg gaagaattgc agctcaaaca gcaaagcaag
     9241 tagttgttca aagaataagg gaagctgaga gagaaatagt gtataatgaa tttatagaaa
     9301 aagaaaatga aatagttaca ggagaggttg ctagagcaaa taaaaatgtt gtttatgtaa
     9361 atcttggaag aatagaaggt ataatgactc aaagtgaaca aataccattt gagaattata
     9421 aagcaggtca aaaaataaaa ttctatatac ttgaagttaa aaagactaac aaaggacctc
     9481 aaatagttgt atcaagaagt catctgggtc tagtaaagag attgtttgaa gaagaagttc
     9541 ctgaaatata tgaaggaata gttcaaataa aatctgtagc aagagaagca gggtcaagaa
     9601 ctaaaatggc agtaagatct atagatgaaa aaatagaccc aattggagct tgtgttggac
     9661 caaaaggact tagagtaaaa aatatagtgg atgaacttgg cgatgagaaa atagatataa
     9721 taaaatatag tgaagaccca gcagagttta tatcagcagc acttagccca tcaaaagttg
     9781 ttagagtaga tatagatgaa gatgaaaagt ctgccttagt aatcgttcca gattatcagt
     9841 tatcgcttgc tataggtaaa gaaggtcaaa atgcaagact agcagcaaaa ttgactaact
     9901 ggaaaataga tattaagagc gaatctcaag cagaacaaga aatgttaaat cttgaaaatt
     9961 ccaaagaaat agaagctgag gatgttgaag ctgaatttat cgaccaagaa gagttagata
    10021 cttcagagga taattttagt gaagaagagt aaggagggat gattttgaaa aaagtaaaaa
    10081 aagtgcctca aagaaaatgc atagcatgtc aagatagaga ctctaaaaaa gagcttataa
    10141 gaatagttaa aaataaagaa ggaaaaatat ttttagaccc aacaggaaga gccaatggaa
    10201 gaggagcata tgtatgtaaa agctctgaat gtctaaaaaa agctataaag agtaaagcat
    10261 taaataaagc ttttaaaata gatgttcctg atgaagtcta tgataatcta cttatggagt
    10321 tagaagaata tgaaaaataa taaagaaaaa gtactttcgt tcttagggct cgcaactcgt
    10381 gcagggaaaa tagtgtctgg tgatgattca actttgcttg accttaaaaa aggaaaagta
    10441 aatttgattt taatcgcaga agatgcatca aataatacta aaaaactttt taaagataaa
    10501 tcaactttta gaaatattcc atatctattt ttttcaacaa aagaggaaat aggatttgca
    10561 ataggaaaat ctccaagagc agttgtagga ataaaagatg aaaatttttc taaaaagata
    10621 attgaactaa tagaaattta aaataaaata gggggtgatc ttgtgtcaaa aacaagagta
    10681 taccaaatag cagaagaatt aaatatatca aatgaagagc ttataaataa gttagctgaa
    10741 ttagatataa atgtaactga caaagattca gtattagaag gtgaagagtt agaattagca
    10801 cttgaaatgt taggtgaaga tttatctcaa gaaaatggaa atgttataga aatagatgga
    10861 aagttgacag tacaagtatt agcaacgaaa ttagataaat ctccatcaga aataattatg
    10921 aagcttatga aaatgggaac tatggcaaca ataaatcaag agataagttt tgagattgct
    10981 gccttggctg ccaaagacta tggctttgaa ttaacagtag ctgaaagtga tgatacagaa
    11041 gctttagaaa tagaagcttt aatggaaatt gaagaagata aagaagagga tttaaaacct
    11101 agaccaccag ttgttacagt tatgggacat gttgaccatg gtaagacatc tttactagat
    11161 gcaataagaa aaactgatgt gatttcaggt gaagctggag gaataactca gcatataggt
    11221 gcttcagaag ttaaaatcaa tggacacaaa atagtatttt tagatactcc aggacatgag
    11281 gctttcacat caatgagagc aagaggagca caagttacag atatagcaat attagtagtt
    11341 gcagcagatg atggtataat gcctcaaaca gttgaagcta taaaccatgc taaagcagca
    11401 ggtgtacctt taatagttgc tattaataaa atagataaac caggtgcaaa cccagataag
    11461 gttaaacagg aattagcaga ccaaggatta ttagttgaag actggggtgg agaagtaata
    11521 gcagttccag tatcagctaa gaaaaaagaa ggaatagata cacttttaga aatggtatta
    11581 ttggttgctg aaatggaaga gttaagagct aatccaaata aaagagcagt aggaactgtt
    11641 atagaagctg agcttgataa aggaagaggg ccagttgcaa cagtacttgt tcaaggtggt
    11701 actttaacag taggtgaccc aatcgttgca ggtgttgcat gtggtaaagt tagagcaatg
    11761 ataaatgcaa aaggtaagag agtaaaaaca gcaggaccat ctacagcagt tgagatatta
    11821 ggtctatcag aagttccaca aggaggagac caatttgtcg aagttccaac tgataagatt
    11881 gctagaagtg tagcagcaag aagacaacaa atagttagag atgaaatgtt aaaatcaact
    11941 caaagattat cactagatgc tctatttagt caaatgagtg aaggttctat aaaagacctt
    12001 aatatagtta taaaagcaga tgtacaaggt tcagtacagg cagttaaaca atctttagaa
    12061 aaattatcaa atgaagaagt tcaagttaaa gtaatacatg gtggtgttgg agctgtaaca
    12121 gaatcagaca tattacttgc agcagcttct aacgcaataa taataggatt caatgtaaga
    12181 ccagttccag gtgcagaatc tctaggagaa aaagaaaatg ttgatataag aacatatact
    12241 ataatataca aggcaataga agatatacaa gcggctatga ctggtatgtt agaccctgaa
    12301 tatgttgatg aagaaacagg aaaagctgaa ataagagaaa tatataaaat atctggtgtt
    12361 ggaacagtag ctggatgtta tgtgactaat ggtaagatat ttagaaattg taaagtaaga
    12421 ttagttagag atagcataat aatacatgaa ggtgagcttg cagcacttaa gagatttaaa
    12481 gatgatgtaa aagaagttaa ttctggatac gaatgtggta tgagttttgt aaattataac
    12541 gacataaaag aaggcgatat agtagaagct tatataacta aagaagtaga aagaaaatta
    12601 taaattcgta tcaaagaaaa tcagttagta aagaaggtcg atggtatggc atcatataat
    12661 agaacaagaa ggatagcaga agaaataaga aaagtcgtaa gtactatgct tataaatgga
    12721 gtaaaagacc ctagaataac atctatggtg agtgtaactg atgtagaagt tacaaatgac
    12781 ctaagatatg cttatgtata tgtaagtatt ctaggtggag atgaagaatc cacactaaca
    12841 ggtcttaaaa gtgctggagg atatataaga agagaagtag gaaaaaatat aaaacttaga
    12901 tatattcctg agatagtatt taaacttgat gattcaatag aaaaaggaat gtatatggat
    12961 agccttataa aaagagttaa tgaaaaaaat gctcaacaaa atgaggatga aaattatgat
    13021 gaataatatg gatagtatta taaactatat ttatgagggt gactatttta tagttacctc
    13081 tcatatcagt ccagatggag acaatattgg ttctacactt tcaatgtatt atacattgaa
    13141 gaatttagga aaaaatgtat attatgtact tgatgatgaa gctccattaa atttaaggtt
    13201 tttggttgaa ggtgtaaaaa tctataagtc taatgaattt aatatgaaga actattctct
    13261 aattgcactt gattgtgggg ataagatgag aatatgtgtg agtgatgaaa tcaaaaataa
    13321 tgcatcaaaa ttaatctgta ttgaccatca tgctagtaat gattcatatg gagatttaaa
    13381 ttatgtagat attgatgcat cttctacatg tgaattagtt tataatttat tagttagatt
    13441 tcagcaaata aaagatatta atattataaa tgaagatata gcaacatgtt tatatacagg
    13501 tcttgtaact gatactggaa acttttcgta ttcaaatgta catgcaagta gctttgaaat
    13561 ggcaaaaaat ttacttgttt taggtgcaca aaaaaatact ataatacaaa atatatatca
    13621 aagcaattct tctggttatt acaaattatt aggagaagct ctaaagggtt tggagatatt
    13681 tgattaaaaa atagctacta actcgtgact ttagtcatga gttagtagct aaaatagtca
    13741 gcatataggg aaacttatat gtagagatag gata
//
LOCUS       DAKBNN010000100        13736 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000100 DAKBNN010000000
VERSION     DAKBNN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.100"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            101..499
                     /locus_tag="OUB02_002383"
     CDS             101..499
                     /locus_tag="OUB02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886136.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(622..1638)
                     /gene="ccpA"
                     /locus_tag="OUB02_002384"
     CDS             complement(622..1638)
                     /gene="ccpA"
                     /locus_tag="OUB02_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HCT8886137.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(1891..2091)
                     /locus_tag="OUB02_002385"
     CDS             complement(1891..2091)
                     /locus_tag="OUB02_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886138.1"
                     /translation="MLDRFFGEEGGFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(2125..2304)
                     /locus_tag="OUB02_002386"
     CDS             complement(2125..2304)
                     /locus_tag="OUB02_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886139.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(2486..2692)
                     /locus_tag="OUB02_002387"
     CDS             complement(2486..2692)
                     /locus_tag="OUB02_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886140.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(2740..3165)
                     /locus_tag="OUB02_002388"
     CDS             complement(2740..3165)
                     /locus_tag="OUB02_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886141.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            3337..3564
                     /gene="acpP"
                     /locus_tag="OUB02_002389"
     CDS             3337..3564
                     /gene="acpP"
                     /locus_tag="OUB02_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCT8886142.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(3708..4712)
                     /locus_tag="OUB02_002390"
     CDS             complement(3708..4712)
                     /locus_tag="OUB02_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCT8886143.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(4726..5628)
                     /locus_tag="OUB02_002391"
     CDS             complement(4726..5628)
                     /locus_tag="OUB02_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCT8886144.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(5823..6998)
                     /locus_tag="OUB02_002392"
     CDS             complement(5823..6998)
                     /locus_tag="OUB02_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCT8886145.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(7085..7930)
                     /locus_tag="OUB02_002393"
     CDS             complement(7085..7930)
                     /locus_tag="OUB02_002393"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HCT8886146.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(8028..8825)
                     /locus_tag="OUB02_002394"
     CDS             complement(8028..8825)
                     /locus_tag="OUB02_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCT8886147.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(8844..9854)
                     /locus_tag="OUB02_002395"
     CDS             complement(8844..9854)
                     /locus_tag="OUB02_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCT8886148.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(9873..10655)
                     /locus_tag="OUB02_002396"
     CDS             complement(9873..10655)
                     /locus_tag="OUB02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCT8886149.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(10714..11850)
                     /locus_tag="OUB02_002397"
     CDS             complement(10714..11850)
                     /locus_tag="OUB02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCT8886150.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(12559..13026)
                     /locus_tag="OUB02_002398"
     CDS             complement(12559..13026)
                     /locus_tag="OUB02_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HCT8886151.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(13105..13647)
                     /locus_tag="OUB02_002399"
     CDS             complement(13105..13647)
                     /locus_tag="OUB02_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886152.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT         4296 a         2583 c         1470 g         5387 t
ORIGIN      
        1 ttatattata tatcactcac aattcaccac ttcaatatta tttctatata taaatagtat
       61 gaatataatt caatatatat ttatagaaag gggttttttt atgaaaaaat tagatacaga
      121 gtcattagaa aaaatggcta aacaaaaaaa tattgacaag gataaaatag aaaagatggc
      181 tgatagttac aaaggaaaat cagaaaatga acttatggag gaacttataa aaataggtaa
      241 gaacctagat ggcagagatg aagtagtatc aaaattcaaa gcatttttag atgaaaatca
      301 acgcaaaaaa ctggataata taatggaaaa aatctcagac gcagaaaacc aaagagatac
      361 taaaccagct aaaactaaga aggctaaacc tactaaaggt aagtcaaatt cacctgcgcc
      421 aaaaaataca ccaggtagtc aacaaaaaaa atctaaaagt ttatttaaga aaactaaaaa
      481 gtctaaccct catgaataga taaaaataaa ctatttaagt aattagataa ttatttttag
      541 ttaatctatt attaaatttg atattattga cataaaaaat caggtagtgc cttaggcact
      601 acctgattga ctttaatagt tttatttttt tctttctgtg acactttctc tatcaacaat
      661 tctataaggt aattctattt tcttattttc aattaattcc ttatttatta attttataac
      721 cattcttatt gctactgctc ccatatcgta tagaggttga tatactgtag taagtttagg
      781 tctatacatt ctagctatct taacatcatt aaatcctgca acagatatgt cttctggaac
      841 cttatatcca gcatcacata tagcatttat agcacctatg gcagcctcat cacctgttac
      901 aaaagccgca tgaggtatta ttccatcatc taatagttct ttcatactat tgtatccact
      961 ctcataatca gttccaccat acttaatcaa actcttgtct aattctatat tgttatttga
     1021 aagtgctttc tcataaccag caagtctttc catttctaaa acagtatcat ctttactcgt
     1081 cattataaaa gctatctttt catgaccttt ctttataaga tgctctgtca tatcaaaagt
     1141 agcttcttcg ttgctagtac ttactgtata tatatcataa tttctagcag ttttacttat
     1201 atatgtcgct ggtattccac aattttgtat atagttgata tgttcttgtt caactatcca
     1261 tgaaatcata actatacctt ccacttgttt tgctctcaat agatttatac tcttaagttc
     1321 ttgttcctta tcagagtatg tattcgctaa aagaatatca tagtcataca ttttagcaac
     1381 ctcttctatc ccatttaaca cctcattaac aaaagaatct gaaacttctg gaactattac
     1441 ccctattaat tgactcttct ttgttactaa gcttctagca agtggatttg gtatatatcc
     1501 agtctctttt ataacctcta aaactttttg tttgacttca tcagttacag gttttgaatc
     1561 atttataact ctagatacag tagatattga cactcctgct tgtttagcaa catcttttat
     1621 cgttatattg cctttcatct tcatcctcct tattttacta aatttatcta aaactgaacc
     1681 aaatttttga aggttcctat tttaactaca tttatataag tttttatttt accataaaag
     1741 caagtacatt taaatacatt atattttata ttatacccta tattcaacaa atttaataat
     1801 attcattctt aatttaaatc atattaaaaa ataaaataaa aaaatgctgg tataaaatac
     1861 cagcattttt ttattctaat gatttaagta ttagcagccg caacctactt caccatcaaa
     1921 gaatccacca catgaacaag ttattagcaa taatattaag aaaggtatcc atgacattat
     1981 gtcaatgtct ctccagcaat cttcaaaagc taagaatagg aagaatagta ctattatcca
     2041 ccatcctcct ccaccaaagc ctccttcttc tccaaaaaat ctatctaaca taacaaagcc
     2101 ctcctttttt taataactgt gttcttattc taagcatcca ccacagcagc aaagtattaa
     2161 taaaactatt aggaatggta tccatgccat tatgtcaaat tctgcccagc tatctccaaa
     2221 agctaagaat aggaagaata gtactattat ccaccatcct cctccaccaa aacatccttc
     2281 ttctccaaag aatctatcta acattatacg ttacctcctg ttgatatttt gttgagcttt
     2341 tcgctcctaa catatactat gcatttggtt tccattttgc taatgtattt ttactatttt
     2401 tttaaatttt ttaatttgaa ctaaaaaagg taaaaccata acttgtataa gttactggtt
     2461 ttacctattt aataatcaat attttttagc aacaacaacc atcatcgaag ttaaataata
     2521 acaatactat taatataacc catatccata tattgtcatc gcataataag tcttctaaaa
     2581 tgtctgtgca taatagtaag aatactataa ctattattat caacatttta ttcccaaaaa
     2641 atccttcgca tcctccaaag aaacgttcca tattgtcttc taattttgtc ataactaatc
     2701 ctccttgaat cattaagaga ctaagttttg tctctttaat taaatatatg tagtaataga
     2761 cagataagtt acttctaaaa agtttttatt gattagttta tttatttctt tttcaccaag
     2821 tttattgtat ctatcaactt catccagata atcttttttc tcctctgata tagtatagat
     2881 taaactttct attgatttta ttttgtactc cttatatctt tctatcaaga aggatgtttt
     2941 tgaattttct attcctttct ttacatgcat taaccttgat atgtagtctt ctttttcatt
     3001 agatacataa ctctcctttt cagctacatc ttcaaggatt aaattggtct ccattacata
     3061 atcctctata cgttgcatat ctaaagttat tgaattataa taaaaattta ctgctataag
     3121 taaaaacatt cctactgaag ttagtaatag tcctacatat ttcatagcat ttcctcctaa
     3181 gttatatttg ttatcaataa agtttattca tcaaataata ctttattact acatttataa
     3241 aattttatta ttttttatag attccatatt gaaaaattct tgatattata ttaaactaac
     3301 attaaaatta attaaaactg ctttaggagg cttaaaatgt ttgagaaaat aaaagaaata
     3361 atagcagaac aattaggggt agacaattta gatgagataa ctatggaagc atctttaatg
     3421 gacgacttag aagctgattc attagatgct gttgaaatta taatggccct tgaagatgaa
     3481 tttggaatag aaataccaga cgaggaagct gagaacttta aatgtattgg tgatatatgt
     3541 aaatatatcg aagaaaacaa atagtatcgt tttgtctaca aatataaata gtctgtgtaa
     3601 acttctacat aataaattat attagagttc gcacagacta gattacattt atttacaata
     3661 tataaaatac acgctcttat atttaattta tatactaagt atataaatta cttttcttca
     3721 taaaatgtaa cattctttat tattatatct atagttccat cttgattttg ttttacagta
     3781 tacttcattg ggtcttcaaa atcagttaga ttccctttta tgtcaaaacc attatccgtt
     3841 tttatacttc tttttttaag ctttttctca acccattttt tatctatact aaaactttca
     3901 tcaagacctt tttcttccat atgctcttta aagctatcct ttaactcatc atctttaatt
     3961 gaattatcaa caaagtcgtt tatatcaact tcatgttttt ccttcagtgt ataatttaat
     4021 atacttctta catcttcagc ttgctttata tcattactaa gagcattggt tatccaattt
     4081 tcagctgtat ttttaaatac cttggtcttg tacttatcat catctatttt tttagcattt
     4141 aaaaattctg ttacaaactt agattctgac ccttcttttt cagcatcctt atctaaaagc
     4201 cttagatgaa actcatcatt tataccactt agactaatca aagctccttg cttttgtctt
     4261 aaagtctctg ggataccaat ttcatttgaa accatttgaa tattaaattt atcatccaca
     4321 aattcaattg aatgagtgta caacttttta taatctaatt tcagtattgc tacactcttt
     4381 tcatctttaa tagtatataa gcaaattgcc aaatcacaag attctagtgc agcattaatt
     4441 ttcataacat caaacaagta agatgcaatt tctttagaat ttttcaaaaa agtactttca
     4501 tcatatatta tttgttcaca acagttttta attaaattgt cattataatc tttaaaaaca
     4561 ccttttctta aatcctcatc ttttgcaatt ctttttatag ctttctggaa aaaaccatcc
     4621 acttcttgat taactttgcc ttcaaagtca tttaatattg gaacatcact atttttgtcc
     4681 aaaacatgta ttataaattt atgtattatc atatatatca ccccattatc ttaatttcga
     4741 tattaactct ttcatatcct ctggtatatc tgctttaaat ttcaattttt cttttgttct
     4801 tggttgttca aactctaact tatatgcatg aagtgcttgc ctatttatta gctcttcatc
     4861 tacataacca tacaactcat cacctattat tccatgacct atatgagcca tatgtactct
     4921 tatttgatgt gttctaccag tttctaagct tacttctaat acagttgcat tttctagtct
     4981 ttctattact ttatagtgag taacagatga ctgtcctctt tcatctatta tcctctttat
     5041 tgaatcttct gtaggtctat aaattggctc atctatagtt ccctcatttt cttttataat
     5101 tccatcaact actgttatat actttttttc aactttattt tcactcattg ctgtagacaa
     5161 agtatgatgt gcataagcat tttttgctac tataactaac cctgaagtat tcatatctaa
     5221 cctatttaca aatcttattt ttacattttc tttttgatta tctatatagt aactaatacc
     5281 atttgctatt gttttatcat agtggctttt tgttgggtga actaccataa acggaggttt
     5341 attcaccata attatatcaa aatcatcata aattatctgt aaatttaaat cttgaggttc
     5401 aaaatttgct ttttcctcgt ctatttttac ttctattaaa tcaccactat acacctttaa
     5461 acttggtttt ttatatactc catttactaa aacactcttt tccctcttca tttttgataa
     5521 tgacctaact gagaaattta gcttgtccaa taaaacttct tttaaagtca tttcttcttc
     5581 atttgtatac gatataagat tatatctttg attctctttt ttaaacaaac ttttacctac
     5641 tttcttttat atcttcctac tttttatatc ttcaaaatca tgatattata tattttacac
     5701 tatttttgtt taatagataa taattatttt taactataaa aacctgtaag aataacaaat
     5761 agttattctt acaggttaaa ttcataatta aatatttaat ttgtaaatat tataatctaa
     5821 aactatctct taactattaa agtagttccc attccaccgc ctatacaaag tgtagcaaga
     5881 ccagttttag catctcttct cttcatttca tataaaagtg tagtaagtat tcttgctcct
     5941 gagcatccta ttggatgtcc tatagctatt gctccaccat taacattaac tttattcata
     6001 tctatattta agtctcttat tacagctaca gattgggcag caaatgcctc attagcttca
     6061 actaaatcta tatcttcaat agtcatatta gcagcttcta aagctttttt agttgctgga
     6121 actggtccat atcccattat tttagggtca acaccagctg ttccataaga aactatagtt
     6181 gcaagaggct ctattcctag ttcttcagct ttttctttag ccattactac tagcatagca
     6241 gcaccatcat ttattcctga tgcattacca gcagtaactg ttccatcttt tttaaatgca
     6301 ggtcttaact tagcaagttt ctccattgta gtgccaggct taatatattc atctttatct
     6361 actacagtgt caccttttct tccttttata acaacaggaa ctatttcttc atcaaatttt
     6421 ccttcagctt gagctttttc agctttattt tgacttgcaa gagctaattc atcttgttct
     6481 tctctagtta tattccattg ctctgctatg ttttcagcag taatacccat gtgatagtta
     6541 ttaaatatgt ctgataatcc atcttttatc attgaatcaa caaaagcagc atcacccatt
     6601 cttgcaccat atctcgcact tggtactaaa taaggagaca tactcatgtt ttcagctcca
     6661 ccaactaaca ttatatcagc atcacctaat gctataagtt gagatgccat tgaaacagat
     6721 cttaatccag aaccacaaac tatatttata gtcatagctg gtttttctac tggtattcct
     6781 gctcctaatg ctatttgtct tgctatattt tgtccaagac ctgctgtaag tactccccct
     6841 aaaagagatt catctatcat atctggagtt atgttagctc tttttatagc ttctttagct
     6901 gctgttaccc ctaactctac cgctgaaact gatttaaatg ctcctccaaa acttcctact
     6961 gctgttctag ctgcactggc aattactact tctctcatat tcattacccc cattaaatta
     7021 gatttttcat atatccgaaa ttgtttttta aacaatttta tttaaacttt taattcccaa
     7081 attcttattt attataatca tagaatccta tcttagtttt tcttcctaat tgattagctc
     7141 taaccatttt agctaaaagt ggatgaggtc tatatttagt atctccaaat tcagtatata
     7201 aaacgttcat tatagctaaa acaacatcta atccgattaa atcacctaat gctagtggtc
     7261 ccattggatg gtttgctcct aatttcatag cttcatctat ttcttcttta cttgcaacac
     7321 catctgcata tataccaaca gcttcattta tcataggtat aagtattcta tttactacaa
     7381 atccaggaga ttcagataca tctactggta ctttattgat actcttagat aattcaaata
     7441 ctgtatcaaa agtaactttt gatgttaact gaccacttat aacttcaact aatttcatca
     7501 taggaactgg attaaagaaa tgcattccta taactttatc tggtctctta gtagaagaag
     7561 ctatttctgt tatagataat gatgaagtat ttgttgccaa gatagtatct tctttacata
     7621 attcatctag taacttgaaa acatctttct ttatattcat gtcttctaca gatgcttcta
     7681 ttattaaatc catatctttt aaatcttcat aattagtagt tgaactaaca tgacttaata
     7741 tttctgcttt tgtagcttca tccatttttc ccttagtaac taacttagtt aaatttttat
     7801 ctaataaagc taaacattta tctatagcac cttgagttct actctttaaa catacatcat
     7861 gtccacaact tgcaaaagtt tgtacaatac cacttcccat agttccacta cctattacag
     7921 ctaatttcat agttataacc tcctgttatt ttagtattta tattctgtta actagattaa
     7981 aatactattt aatctagtta acagcaaata tatttttaat tagattttta cccttttata
     8041 aagttagctt ctctcttttc aacgaaagct gacattcctt ctttttggtc tttagttgaa
     8101 aaacaaagac caaataaatt agattctata tctattccag tatttatatc agtttgagca
     8161 ccaagttgta ttgcttcttt agagtatcta actgcaagct gagcattttt agctattatc
     8221 ttagctaatt tctcaacttc ttctattaaa atgtctggct caacgactct atttactagc
     8281 cctatttttt cagcttcatc agcttttata acttgacctg taaagattaa ttcttttgct
     8341 tttgccattc caaccaatct tgtaagcctt tgagttcctc catatcctgg agttattcca
     8401 agagttactt ctggctgacc aaatttagct ttagcagatg caattcttat atcacatgcc
     8461 attgcaagtt cacatcctcc acctaaagca aatccgttta cagcagctat cactactttt
     8521 ttactatttt ctatttctcc aaaagctttt gctcctaaga tactaaaatc tttagcagct
     8581 acagcatcta aatctttcat gtatgcaata tctgctccag ctacaaatgc ctttccttcc
     8641 cctgtcaata ttacaacatc aatagtttca tcattattaa tatctacaaa tacttcataa
     8701 agttcttcta aagtctttga atttattgca ttaagggctt taggtctatt cattttcact
     8761 gtacatatat ttccatctac ttcaacagct acattctcat aaactttaac atcacttgta
     8821 ctcataatac cctccccaag aaattagtta gctaaaactt cacctttttc ttttgcaaca
     8881 cttaactgac tgataagttc tggaagcact ttatgaacat ctccaactat accaacatca
     8941 gcatatttaa atattggagc ttctggattt ttatttatag caactataaa ctcagcatct
     9001 tccataccag ctatatgttg tattgctcca gatataccac atgctatata aaggtctggt
     9061 cttacagttt taccagtttg accaacttgt cttgctttat ctaaccaacc tgcatctata
     9121 gtggcacgag aaccagaaac ttctccacct ataatttcag ctaattcata aagtatgtct
     9181 aagttttctt ttccacccat tccacgtcca gcagaaacta atatcttagc atcttctatt
     9241 ttaacttgtt ttttagcttc ttttattact tgtacaactt gaactaattt atcagcatca
     9301 ttaaattcta ccttgaaacg gttaattaca gcttctttag tttcatcagg ttcatttttc
     9361 ttcataaccc ctggtctaac tgtagacatt tgaggtctga aatctttaca aactattgtt
     9421 gccattatat ttccaccaaa ggcaggtctt gtcattaata ataattttgt atcttcagct
     9481 actgcaagac ctgtacagtc agcagtaaga cctgtatgta ttctagcaga aactctaggc
     9541 gctaaatctc tacctattga agttgcacca aataatacaa ctatagggtc agctgctttt
     9601 attgcttcat aagctgcttt tgtatatggt tcagttgtat acactgctaa agcttcatca
     9661 tctactacta ttacctcatc tgcaccatag tgtgctaatg tatctattaa accttctacc
     9721 ttactaccta aaagtaatgc agaaactttt gtatcataat cttttgctat ttctgtagcc
     9781 tttcctagta attctaaaga aacagtttga attacatttt ctctttgttc tattactact
     9841 aaaacgttac ccattcttta tcccccctta aattatatga tatacttctc ttttaattta
     9901 tctatgataa ttccagctga tgttttcgca tcttcattgt atattgtacc agctggttta
     9961 actgattttg taaatgattt aaatacacta gttggagaac ctttaagacc taaattagaa
    10021 gggtcaactt ctatatcttt tacagtccat gtttctacta catcattttc gaaagcatca
    10081 tatattcttc caactttcat gtatcttggt gtgttcatat ctttaagagt tgttataagg
    10141 catggcattt taacttttaa gtcatggcaa caatcttcaa attgtctttt tactaataca
    10201 tattcacctt cagtttttat ttcttcagca tatgttattg atggaagatt taaatgttca
    10261 gctatttgag gtccaacttg tgcagtatct ccatctatcg cctgtcttcc agctattata
    10321 atatcaaaat ctatattttt taatgctcct gctaatgctg atgaagttgc ccaagtatca
    10381 gcacctgcaa atgctctatc tgttaataat atacctctat ctgcacccat tgctaaagct
    10441 tcttttaaag ccatatctgc ttgaggaggt cccattgtta taacagttac atgagcaccc
    10501 atttcttctt ttaattttat agcttcttct aaacctgctt tatcatcagg gtttattata
    10561 cttggtactc catctctaat taaagtacct gtattaggat ctagtttaac ttctgttgta
    10621 tctggaactt gttttataca aacgactata ttcataaatc cttcctccat atctatttta
    10681 atatcgacct attgtgtata ttttaatatt tatctatttt aatagttttc ctgaaataac
    10741 cattctttga acttcactag ttccttcata tatttcagtt atcttagcat ctctcatcat
    10801 tctttctact ggatagtcac gagtgtatcc atatcctcca tgaagttgta cagcttttgt
    10861 agtaacttcc atagctgttt cagctgcaaa taattttgcc attgctgctt ctactccata
    10921 aggttttcct aagtctttat ttatagctgc ttgatataca aggtgtctag ccgcttgtac
    10981 cttaacttcc atatcagcta attggaattg tgtattttgg aattttgata atggtctacc
    11041 aaattgtact ctttctttta catatttaac agtttcatca agagcacctt gtgctaaacc
    11101 taaagcttgt gcagctatac caattctacc accatcaaga gtagacattg ctatcttaaa
    11161 tccttgacct tcttttccaa gtaaattttc tttaggtatt ctgcaatcct caaatattaa
    11221 ttcactcgta gctgaacctc ttatacccat tttcttttct ttaactccaa agctaaaccc
    11281 aggagttcct ttttcaacta taaatgctga tattcctttg ttccccttag atttatcagt
    11341 cattgccatt actacatata tgtcaccagc tattgcgttt gttataaata tttttgagcc
    11401 attaagtatg tattcatccc cgtctaaaac agcagttgtt tgttggccag acgcatctgt
    11461 accagcatta ggctcagtaa gaccaaatgc tcctaatttt tctccacttg ctagtggtct
    11521 taagaatttt tgtttttgtt cttcattacc atattgatat ataggccatg agccaagaga
    11581 tgtatgagct gataatataa ctcctgtagt accacaaact ctagacaatt cttcaactgc
    11641 cattatatat cctacagtgt ctccaccttc tccaccatat tcttttggat atggtatacc
    11701 catcattcct gcttttgcca ttttttccac tgtttcataa ggaaatcttt cttcttcatc
    11761 aagttctgtt gctaaaggtt taacttcatt ttcagcgaag cttacatata gctctttaag
    11821 catctgatat tttttagaat ttaaatccat aatagcctcc tcagtatttt ataaatattt
    11881 tattttcatt tattaattaa aatctcggca acatctataa taaggtattt gaatatacgt
    11941 cgagaaattt ataaccttaa tcaaactacg ctctatattt atatgtgatt atactaacca
    12001 attatgacat taatttaatc ataaattgtt aatttaaaat caactatact taaaaaaaat
    12061 ctcgcttttt ggaattttgt gaaatttcaa gttagaataa gatttctttc acactggtta
    12121 gagataaaat aatttgttta cgatatttat attagggtat attaaacatt aaatattgtc
    12181 tgtacatctg ttttttagcc atgtacagaa gatttttatc gtataaaaat tattttaata
    12241 gagctaaata atcatatttt tatctgacta ttttttattt ttaaatatct tacatctata
    12301 atagtatcac tcaaaggata atttttcaat aatttctaat tattttttta acaatattta
    12361 ttacattttt tccacttttt tgtcattttt ttattgtttc gaaaaaaaat cagaattttt
    12421 taactttatt ttggattatt agtttaaatt agattttcct cttagattta taaatattta
    12481 cacaaaaaaa ctacctctgt atagattgta tacagaggta gttttttact aattcctact
    12541 atagtttact atattttttt accaattctt acaaacatct acccattttt cacacttaga
    12601 aaacttctcc atttcttcac atgtaagttc tattgcagag ttactactgc cacatgcagg
    12661 aaatactgta tcaaatcttt tcattgaatc atctaaatag acttttatag aattttttaa
    12721 accaaatgga cagactccac caatagcatg tccagtaaac tccaagactt cttctggtgt
    12781 caacatttta gccttacaat taaattcagc cttatacttc ttattatcaa tcttagcatc
    12841 accagctgtc acaattaaaa ttgcatcatc tcctatttta aatgagagtg tcttagctat
    12901 tcttgctgga ataaccccag ctgcttcagc tgcaagttcc acagtagcac tagattgttc
    12961 aaattctaag atactgtctt ccttgccaaa ttgtttaaaa tactctctta cattacttat
    13021 tgacattaaa tacccctcct agtctgaact tttataaatt tcatattatt cttaattata
    13081 tctataattg atttattttt caaattattc cataatatta gcattccatg tatcattatt
    13141 ttttccaact actagataaa gtccttttgc cattggaata ttgacaatat attctccttt
    13201 atactctaca ctcataccag attcattaaa gtacctataa tcaaaattta gtccttttgg
    13261 aattacaaaa tcaacataac cttgattatt tggttttatt ccatatttca gagttctttc
    13321 agttatagaa tttttttcat taaataaatc tacatttaca tatgcagaac acttattcat
    13381 tcccttttta ttttccacac aattttcaaa cttcatttta gcactgttta atttataact
    13441 atatttacta ttattgctta ttttaaatct tacaatagta ccaacatctc tacttctcct
    13501 gctatcaaaa tcaaaaacaa tttcctctaa atgatttatt tcataatctt tgacacttac
    13561 catatctcta tatactaatt tatttatagc tatgaatagt gtaaaaatcc ccaaaataaa
    13621 aaatgaaact tttataaact tcttcatttt tagtctcctt atattttgtg ctattgtgct
    13681 ataatattaa tcaaaatttt aataattaaa cttattcttt tttgaaatct taatag
//
LOCUS       DAKBNN010000101        13697 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000101 DAKBNN010000000
VERSION     DAKBNN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.101"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(403..954)
                     /locus_tag="OUB02_002400"
                     /pseudo
     CDS             complement(403..954)
                     /locus_tag="OUB02_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
     gene            1162..1252
                     /locus_tag="OUB02_002401"
     tRNA            1162..1252
                     /locus_tag="OUB02_002401"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1196..1198,aa:Ser,seq:gct)
     gene            1340..2128
                     /locus_tag="OUB02_002402"
     CDS             1340..2128
                     /locus_tag="OUB02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HCT8886153.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            2149..2889
                     /locus_tag="OUB02_002403"
     CDS             2149..2889
                     /locus_tag="OUB02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCT8886154.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            3065..3439
                     /locus_tag="OUB02_002404"
     CDS             3065..3439
                     /locus_tag="OUB02_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886155.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            3531..4991
                     /locus_tag="OUB02_002405"
     CDS             3531..4991
                     /locus_tag="OUB02_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCT8886156.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFENFKAELENAIKK"
     gene            5044..8073
                     /locus_tag="OUB02_002406"
     CDS             5044..8073
                     /locus_tag="OUB02_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8886157.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNSLIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            8150..10216
                     /locus_tag="OUB02_002407"
     CDS             8150..10216
                     /locus_tag="OUB02_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCT8886158.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDENDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    10599..10809
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11035..11850
                     /locus_tag="OUB02_002408"
     CDS             11035..11850
                     /locus_tag="OUB02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCT8886159.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            11878..12555
                     /locus_tag="OUB02_002409"
     CDS             11878..12555
                     /locus_tag="OUB02_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT8886160.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            12614..13336
                     /locus_tag="OUB02_002410"
     CDS             12614..13336
                     /locus_tag="OUB02_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT8886161.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         5537 a         1367 c         2413 g         4380 t
ORIGIN      
        1 aatgactgaa taaaaaacga ttgtgtcaaa aacaaaaaat aattaagtgt aagaatgatt
       61 gtatcaagaa tataaaatga ttcaacaaaa aacgattgtg tcaaaaataa aaaataatta
      121 agtgtaagaa tgattgtatc aagaatataa aatgactgaa taaaaaacga ttgcgtcaaa
      181 aataaaaaac aattaaatgt aagaataatt atgtcaaaaa tataaaataa attaaaagaa
      241 aaagattata ttgaaggaaa aagatttgaa ataggggtga actttacata ttatagttca
      301 cccttataca aaaatataaa atataattta ttataggaaa atttaaaaaa atcagtctat
      361 ttaatttata ctgatatatt gtaggatttt acaaacaaac tgttatataa ctttttttat
      421 ctccaatttt gattttaaga tatctaagaa tttataacaa tcttctggat atatttctcc
      481 catatttgct acttggaata tgttagcatc taggaatttg ccttttcctg gatagacaac
      541 atagccactt tccctttcta gccctaatat aaactcattt acattgattt ctttaggtag
      601 aaatacagat gttactgtat tagacatata cttatctgga agtaaaaatg ttagacccaa
      661 ttcttccata ccattcctta aaatagcaga acattctttg tatctttcta ttttactgtc
      721 atcttccaaa aactcagtta aagcttcatt aagggcaaat attaaagtaa cattaggtgt
      781 atttggtgtt tgagaagatg atttagcaac tttatagtgt ttataaagat taagatacac
      841 attcttacat tgattttctt taatattttg taaaatactc tctttagcac aaaggtatgc
      901 agaacctgga aatgctccaa gagctttacc accaacacct gttattatat ctattaagtc
      961 agagtttagg tatatttgtt aatgtatgta tatttatcaa ataatattat aagtgttatg
     1021 caagataatt ttaccatatt taaaacttac atttaagcaa aaaagtaaaa atagatgttt
     1081 aataattcaa aataactcaa attttataaa acaattgact aaaatatgat tttattatat
     1141 aatttactag tatagagata tggagaagta ctcaagtggc tcaagaggat cccctgctaa
     1201 gggattaggc tgggtaaccg gtgcgagggt tcgaatccct ccttctccgc caattattaa
     1261 aggctatttt tattaaagat agtcttttta taatgtcata aatgattaga tatgtataac
     1321 taaagaaagg agggaataga tgacaacttt aatttttttc atagtgggga tagcatcttt
     1381 ggtagttgct tttgttattg atgggggaca tgttggagca ttatttgtat tttcatcagc
     1441 acttgttgtg tttggtggaa ctataggagc tgttggagct tcgactccat tccctgtgtt
     1501 taaaaagtct ataaagataa tgttattagc ttttaaaaat aaaaagccta ataacataga
     1561 aaatataatc tatttcaaag aaattgcaac aaaggctagg aaagaggggc ttcttagtat
     1621 tgacaaagaa ataagtgaga atgatgaatt agaccctttt attagaaaag ggcttgaact
     1681 tatggtagat ggagtggatg cagttactat aagaaatata ttggaatcac agatacagat
     1741 aacatcaaaa cgacataaag aaggaatagc aatttttgat tcagcaggtg gatatgcacc
     1801 tacactgggg ataataggta cagttatggg attagtacat attttaggta atctttcaag
     1861 taatccagat gcattaggac ctcaaatttc tgttgcattt ttggctactt tgtatggtgt
     1921 tggttctgcc aatttaatat ggttaccaat agcatcaaaa cttaaggcta taaatcaaca
     1981 agaagtaagt gaaagacttt tggtgataga agcaatatta tccatacttg agggtgataa
     2041 ttctaattta atgacagaaa aattaaaaat atttttaaat tcaaaagaac taattgaact
     2101 tgaagaaatt ggtggtagag ctgaataata taaaaggatg gaatgtttat gttgtatgat
     2161 gaagatgaaa aagaagaaaa caatgagcga tggcttttaa cttattcaga cctcataaca
     2221 ttacttatga ttttctttgt aataatgtat tctatgagta atgttgatgc ggagaaatat
     2281 aaacaattat ctcaatctct aaattctgct tttggtggtt cttctggagt tattgaaggt
     2341 ggtaatagca agatagaacc agtagttgaa ccaggaagta atgatttaga tactttacaa
     2401 aatgctaagt ttaaaaaaat tggtgaagaa attcagaagt atttaaacga aaatggtatg
     2461 gctaattcag tatcattaag agttcaagat agaggattag taataagcct taaagataca
     2521 atactatttg atacagggaa agctattgtg aaggataatt ctagagataa gattattcaa
     2581 ataggtaaga tgttaaatga aatgaatagt tacatgagag tagaaggcca tacagacaat
     2641 atgtctataa aaaattcaga gtttaagtca aattgggatt tatcagttat gagagcaacc
     2701 aatgttgttc aacttcttat tgacaatgca ggtatagcac cagataaatt atctgctgta
     2761 ggatatggtg agtttagacc aatagcagaa aattcatctg aagaaggtag gtcaaaaaat
     2821 agaagagttg atatagtttt agttgatagt aaatatgata atgtagaaaa tgtttcaaag
     2881 gataaatagt taaattagct gtctcaatac agaattaatt ctaaaggaga tggctttttt
     2941 atgcgaaaat caaaaataga tacatagtaa gttacctttt tgtaggaata tatggtatct
     3001 taaatatata atatttaaat aatgtataag ctcaattaaa aaatttatag aggatggaaa
     3061 ataaatggga aataaaaata aaaatactaa atataaagat aaaacaagag aaaggtataa
     3121 taaaaaagtt gagaatttaa ataaagaaaa attaaataaa attaataagc aatctaagaa
     3181 ggtagataag attgatgata ttattaataa atctagtatt gtattatgtg tacttctagt
     3241 agtgttttca ttgctttact tatttaaact ggttcaactt aatataataa tgatactttt
     3301 agtaattaca gtagctattc aacaaggatt ggtgtcattt ggtgcattta gaaataaaaa
     3361 ttatgtaaaa gctttatttt ctatatttgt aatagtatgt ttcttaattg cttttaaata
     3421 tgttgtaaat cctgtttaaa tatatcaata aacgttctat aattaatata aatagtgaag
     3481 ttacagtttt aatgctatta taaattttat aatttgaagg agtagatttt atgaataggt
     3541 gtaagtttgc aaaattaatt atcacatcaa tgattgtttt attacttaca ggatgtagtt
     3601 taaagaagga agataattat aagttagacc cagaaaatcc gacatcaata caaatttggc
     3661 attattataa tggacagcag aaaatggctt ttgataagct tgttaaggaa tttaatgaca
     3721 ctgtaggtgc tgaaaaagga attattgttg aagcattcag tcaaggtaat gtagttgatt
     3781 tatcagagaa agttttagat tctgcaaata aaaaagttgg ttctgaagat gtaccaaata
     3841 tatttgcagc atactcagat actgcatatg aaatggataa attagggtta ctagtagata
     3901 tggataagta cataagtaaa gaagaattaa atgaatacat agacgaatat gtagaagaag
     3961 gcagaataaa ttctaaggat gagtttaaac ttttcccaat tgcaaaatca actgaaattt
     4021 tgatgttaaa taaaactgat tgggataaat ttgcaaaagc tacaaattca aatataaatg
     4081 atataaaaac aattgaagga ttagtagaga catcaaaaaa atattatgaa tggacagata
     4141 gtttaacgaa agagcctaat gatggtaaag cattttttgg aagagatgct ttagcaaatt
     4201 atattattat aggaagtaaa caactaggag tagaaatttt ttctgttaaa gatggtaaag
     4261 ttacactaaa tattgatgaa aaagttatga aaaaactatg ggataatttc tatgtaccat
     4321 atataaatgg atattttgct tcttatggta agtttcgttc tgatgatgca aaaacaggtg
     4381 atatattagc tcttgtaggt tctagttcag gagcagcata ctttccagat agagtaatgc
     4441 taaatgataa tgagagttat aatattgaaa catctgtttt tcaagcacca cgttttaaag
     4501 gagaaggaaa atatgctgta caacaaggtg caggaatggc tgtaacaaaa tctactaaag
     4561 aagaagaata tgcatcagta gaatttttaa aatggtttac agataaagaa cctaatataa
     4621 aattctctat tgcttctgga tacttaccag taaagaaaaa aacgaatgat ttggatgtta
     4681 taaaagaagt tatgaagaaa gataatttga gtttatctga taaagttgaa ggttcaatag
     4741 cctcatctat tgaacagatt aacagttgta atttatatac taataaagca tttgatggtg
     4801 gaactaaagc tagacaaata cttgaatatt caatgcttga taaagctaaa gaagattcaa
     4861 ataaaataaa tgaattagta aaaagtggaa cttctaagaa ggatgcagta gcacaattta
     4921 ataatgataa aaattttaaa acttggtttg aaaattttaa ggctgagctt gaaaatgcta
     4981 taaaaaaata gatgaattgg cagttagtgt gttgacgagc acaaaggtat aggtggaatg
     5041 ggtatggaaa acaatctaag aaaaaacaat tcaataatga aaaagatgat atttccccta
     5101 attattgtta tgctaattca aacaagttta ttttgtgcca caattttatg gggaggaact
     5161 attaagaaat taaatgataa ttcgtttgac atcttaaatg aaagagtaat taatcgtaaa
     5221 aattatatac aaaatgaaat gttacaaaga tggtcaaata caagtgaaac ggaagctaca
     5281 attaattcct ctgtaaaaaa agtattaaat gaaaaaaatt ccttaattga ggatgtaggc
     5341 aacaatgaag aaattaacaa agatattata aaaagcatat caaatgattt aatatatctt
     5401 ttgagaaaaa attctgttac aggagctttt ataatcctaa gtgacaaaaa tattaacgat
     5461 aagtcaatag atagtaaagg tgaaataagt aggacaggat tgtatataag ggatttagac
     5521 cctaagttta atccaaatga taattctgat ttgttaattg agagaggttc atcagatata
     5581 gcaagaagtt ttggaatatc tatggacagt tattgggaac ctaagttttc atttaaaagt
     5641 gaaaatgagc aagatggaga tgattttttt tataaaccat ttagagctgc aattgataat
     5701 ccaaatgtat caagttctaa tttaggatac tggggaagta agtttttatt gacaaatgca
     5761 ggtgataggc ttatgacata tacagttccc cttatttatg aagatggaac tgtatatgga
     5821 gtacttggaa tagatttaag tgtaaattat ttaagagaat tacttccata ctcagagata
     5881 aatgggaata aaaagggtaa ttatatttta ggtgtagata gaaataatga tatggccttt
     5941 gaaaatgtaa tttcaaatgg atactcatta aaagaaaaat tttgggatgg tttacattct
     6001 aatttagagc agaaggaatc gtataaaaat atatataaga ttaaaaataa agataatgaa
     6061 tgtggtaatg gcaacattta tgctagtgta caatacttag atttatataa ctccaatact
     6121 ccatttgaaa atgaacgttg ggctcttttg ggtattattg aagaaaaaag tcttttgtca
     6181 ttttcaaaaa gggtagagaa attagttata atatctatag caatttcttt attaataggt
     6241 ctaataggta tttatattgc atcaagatta tttacaaaac caattgttct tttagccaac
     6301 aaagttagaa acagtaatcc atcaaaacca gtaaatcttg ataagataaa tatatctgaa
     6361 atagatgaat tatcttcagc tatagagttg ctcagtgcaa atgttgcaga ttcttcgtca
     6421 aaattatcac agattattga aatggtagat atgccaattg gagcttttga gttatacaaa
     6481 aacgcagaga atgttttttg tacaaaagaa ttttttagcg tgtttactac tgaaatcaaa
     6541 tttgaagaat atggatatat ggaaaaaaat aagttcctga aaaaattaag gggactagat
     6601 aaatatttgc aagaaagtta tgaaacagaa aatacatata tatataagtt tgaatctaaa
     6661 gataaaaaac ttaagtggat aagaatgaat atctttgaag atgagctaaa ggttcttggt
     6721 gttgttgttg atgtaacaaa agaatttatt gagaaaagga agatagaata tgaaagagat
     6781 tttgatattt taacgaatct tttaaatagg agagcttttc attcacagat acaagaaaaa
     6841 tttaagaata aagatgaact taagatagca gcatttatta tgtgggattt agataatcta
     6901 aaatatatta atgatacata tggacatgat gctggtgatg aatatattag gtgtgctgca
     6961 gacatactta agaagtttat atcatggaat ggtatagtat ctagactttc aggtgatgag
     7021 ttttatgtat ttatatatgg atatgaaagt aaagatagta taagagatat tattaattct
     7081 gttaagaaac agatgaataa taccttatta aaacttcatg atggaacaga atttaaaata
     7141 agggcatcag caggaatttc ctggtaccct gatgattcaa ccaactatga tgaattaatg
     7201 agatacgcag attttgctat gtatcaaatt aaaaatacaa tcaagggtga agttagtgaa
     7261 tttgatatta atatatataa taaggattca ttcttattga gtaatagaga agaattaaac
     7321 aagttaattg atggtcaatt agttgagtat gcatttcagc ctattgtaga ttgtaaaggt
     7381 aaagtttttg catatgaagc acttatgaga cctaaaataa aaacttttag ttcaccaata
     7441 gatgtcatta gattagctcg ttcacagtct aaattatatc aaattgagcg tattacatgg
     7501 tttaaagcac ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg caaactgttc
     7561 ttaaattcta ttcctaataa taatttgtca aaagtagatt ttgaggaaat tgaagattta
     7621 tatggtgaat atttaaatag agtagtacta gaaataacag agaatgataa gattaatgaa
     7681 gattttattg agaaaaagaa agcatatata gaccattgga atatagaaat tgctttagat
     7741 gactttggga caggttacaa cggagatgct gtactattat atataatgcc agactatgta
     7801 aagattgata tgtctattgt aagaggtatt gataaagatg aaaacagaca gaaaatatta
     7861 aaaaatctta tctcatattc taacgaaaga aatattaaag tgatagcaga aggtgtagaa
     7921 acaaaaggtg aaatggaaac actaattaat cttggagtag attatatgca aggctactat
     7981 attagtaaac catcatttat acctgaagat ataagcgaca aaattaaaaa agaactcaag
     8041 atatgtcatg aaaataggaa tgatgtggtt taatagagga aaaattaaat ttatttttat
     8101 gaacttttag tttataatta aaaagaaaat aatataatta taaatcaagg tggtggagct
     8161 aataatcaca acaaatatag atataagaaa gttaaatgaa aatcaattag aagcagttga
     8221 gcatatagat gggccttgca tgatacttgc agggcctggt tcaggtaaga ccagagtaat
     8281 tacatataga atagccaata tggttgtaaa caaaaacata gcaccaacaa gaatcttggc
     8341 aataagtttt accaaggcat cttcaataga aatgaaaaat agagctttga atttaagtga
     8401 tgatataaga cttaataagg taacctatgg tacttttcac tctgtatttt ttaaaattct
     8461 aagatacttt gaaagataca acttagacag tatatttgat gaaaagtcaa agcgaatgac
     8521 aatcaaggca atcctaaaaa gtttaaatat agagaatgct gatgatgatg agaatatagg
     8581 acaagttata aatgaaattt cctatgtaaa aaatgaactt atggataaaa atgaatttaa
     8641 ttcagaagta ttaacaaaag atgagttttt aaaagtatac aacttatacg aagaacaaaa
     8701 atctaaagtt aataaaatag attttgatga tatgcttata agaacttatt atttactttt
     8761 gaacaataag tctgcactag aaatggttag aaatgtgtat aaatatatat tgatagatga
     8821 atttcaagat ataaataaag tacagtttga agttttaaag ttaatttgta gcccattaaa
     8881 caatatattt gcagttggag atgaagacca aagtatatat ggatttagag gtgctagacc
     8941 agattttttg cttgaatttg aacaatactt taataatact aaaaaaataa tattagatat
     9001 aaattacagg tctaaaagtg agatagtgca tactgcaaat agacttatcg acaaaaataa
     9061 aaatagatat gaaaaaatca ttaagtgtag tcagggggat ggaggaagtg ttacctatat
     9121 ttctccgcat gattcagagg aagaagcctt atatattgcc agggaaataa ttgacgaaat
     9181 acaaaaagat tatgtagagt attcagattt tgcagtaatt tatagaacta atatacagtc
     9241 aagagcatta gtagatgtat ttatggatat gaggattcca tttgtagtaa aagactcagt
     9301 aattacaata tatgaccatt gggcaagtca agacatacta gcctatttaa gaataggtat
     9361 taatccaaaa tcaaataaag attggttgag aattataaac aagcctttta gatatatatc
     9421 caaagacagt ataaatatgg ttaaggatga aaatgacttt ataacagcat taataaataa
     9481 gtgtaatctt catcctaaac aagttaaaac aataaatgat ttggaaatag atttaagtta
     9541 tttaaatact ttaaatccta aaaatgcaat atcttatatt agaactagtc ttgattatga
     9601 tagatatgtt ttagattatt gtagcaacag aaagataaag actaatggac ttgtagagat
     9661 attaaatgaa cttgagagtt cagcaactaa ttttaatact gttacagagt ttttagagca
     9721 tattgataga gtaaagactg agttaacgga aaataacaag aataagcaga ctgagggtgt
     9781 catatttaca actatgcata gtgcaaaagg attagaattt agaaatgttt atattatagg
     9841 tgtaaatgaa ggaacaatac ctcatgaaaa atcatatgat atttgtaagg aagagaaaaa
     9901 agaagaacaa ttagaagaag aaagaagact tatgtatgtt gctattacaa gagctgaaga
     9961 aaagttatgt ataagctcta ctttaaataa gtatggtaag aaagttgata aatcattatt
    10021 tattaatgat ataaaatctc caacgaaaaa agaaattgac agtattggtg taggagataa
    10081 ggtataccat aaaaaatttc atgaaggaga aataattaaa aaagatggaa ttatgtttac
    10141 aataaagttt aaagatagag agcgaatcct ggatttgaaa acttgtttat tgaagaatat
    10201 aatttatact atttaattta gaatctaaat ttagatagaa atataatgta ttatttatta
    10261 tgtgcaagac aaatagatat gtgtaaagtg tattatttaa cttattagaa aaagaaataa
    10321 gggtttaata tattatttat agttgtttag aaaaagcaac aatgtggaaa atattattta
    10381 gtattattaa agaaaagcaa caatgtgaaa gatattattt agtattattt aagaaatagc
    10441 taaaaatatg aaatatacta tttataatta cacaaataat atcaataaat ataaacagta
    10501 gtatttagat aatttacaag taaaaattat taaaaaaata aaaaacttat tgacaaaaat
    10561 attataagga agtacaattg tattaacaat ttaaggaaac aaatccaatg atagagaaag
    10621 tagcatatat ggatttacag cgagttaggg gttggtgtaa gcctagcaat gaagtctatg
    10681 tgaagagagc tctggaggag attatcagaa actttagtat gataattggt ttacccgcct
    10741 taagggattg agaggataag tacttactta tcaaaaagag tggtaacgcg gatataattc
    10801 gtctcttagc tgtaaagcta agggactttt ttgattttaa aataaaaata tccatacagc
    10861 ttaaaagcta acagtcacaa attttgaaag taatataagt atttatcaat aagagtggta
    10921 acgcggtaaa gtcgtctctt agtcattaaa gactgagaga ctttttttgt aactcgaaac
    10981 atttaagtat taaaatctga gggaaattat aattaaaaga gaggggtagt taaaatgaaa
    11041 gtatttaaaa aattattaag tttaggatta gtattaggat tgacattatc gctagtagga
    11101 tgtagtggtg gaggagaaaa aacaaagtta gaacaaataa aagacaatgg aaaattagtt
    11161 gttggtacta gtgcagaatt tcctccattt gaatttcata aggtagtaga tggaaaagat
    11221 tcaataaaag gattcgatat aatgttggct gaagaatttg caaaagagct tggtgtaaag
    11281 gttgaaatta aggatatgtc atttgatgga ttaataggtg ctttaaatgc agaccaagtt
    11341 gatattgttc ttgcaggtat gtctccaaca ccagagagag aaaagagtgt tgatttttca
    11401 gaattatatt atttaagtag aaatgcagtt atagttaaag atgcagatat agacaaagta
    11461 aaaactgaag atgatttaaa gaaacttaga gttggagttc aagcaggaag tatccaagaa
    11521 gaatatgtag ttaacacttt aaaaatgaca actacaaaat ctttgaaagc aatacctgat
    11581 ttaattacag aattaaaaaa tggaaacata gatgcagttg ttactaatga agcagtttct
    11641 ttaataaatg ttaaaaaata tgatggaata aaaatggcaa atactgaagt tgggaaagat
    11701 gtaacagaag gtatggcagc agcaatcaaa aaatctgaca ataacaaaga ttttatagaa
    11761 ttattaaaca aaaagataaa agaattacaa gatggcaaga agatagaaga atttttaaat
    11821 gaagcatcta ctgaagctgc ttcaaattag taagaaagtt atgtgggaga tgaaattatg
    11881 agtttagatt ttagtttttt aagtagattt gggacttctt ttttggaagg aacaggtgtg
    11941 acagtatcaa tttctcttgt ggcattatgc tttggattta taataggtat aattatctgt
    12001 atggcaaaaa tatcaaagag caaagtatta agagcaatat catcaatcta tattgaggtt
    12061 ttaagaggga caccattatt agtacagata tatatagtat ggtttggttt acctcaatta
    12121 ggaataagat tccctatgtt gtttggtata ccaagtgaat ttatagctag tgcatttgca
    12181 ttatcagtaa actctggtgc atacgtggca gagatactta gatcaggtat acaatcagtt
    12241 gataatggac aaatggaagc aagtagatca ttaggattaa attattggtc aactatgaga
    12301 tatataataa tacctcaagc tataaaaaat atattacctt cattagcaaa tgagtttatt
    12361 acacttgtaa aagaatcttc aataatatct gtaataggag ttgttgagat aatgcgtact
    12421 gctgatatag taaaaaatgc agcatttaga gcattagagc cattaatagt agcagcagca
    12481 atttactttg ttataacatt tactctatca agattagttg gattattaga aaagaaacta
    12541 tctgtgtcta actagatgag agcatgttta gcatttaggg aaaacataga atagtaaaaa
    12601 ggaaagtgat aatatgatta caataaaaaa tttaagtaaa tcttttggag atttaaatgt
    12661 attaaaaaat atagacttag aaatcgctaa aggcgaaata atggttatag taggtccaag
    12721 tggttctggt aaaagtacac ttcttagatg tatgaattta ctagaaatac ctacaggtgg
    12781 agaaataata tttgaaggaa aaaatttggt cgataaaaaa actaatattg atgaggttag
    12841 acaaaatata ggtatggtgt ttcagaactt taatttattc ccacataaga ctattttaga
    12901 taatataaca ttagcaccaa ttaaattaaa aaagatgact aaggaagaag cagaaaaaaa
    12961 agcagaaatt ttgttgagta gagttggtct tttagataaa aaagattcat atccatcaca
    13021 attatctgga ggacaaaagc aaagaatagc aatagctaga gcacttgcta tggaaccaga
    13081 tatgatgttg tttgatgagc caacatctgc tcttgaccca gaaatggtaa acgaagtttt
    13141 ggatgttata aaagaacttg ctaaagaagg tatgacaatg gcaattgtaa ctcatgagat
    13201 gggatttgct aaggaagttg cagatagagt tatttttata gatggaggaa gtatacttga
    13261 agataatact ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa aagcattttt
    13321 agcaaaagtg ctataatata taaaaaggat gttattgata ttagaaattc aatacatcct
    13381 tttttagtga tggttaatat taattttaaa attaaagtta tattaatatt aactttattt
    13441 ttacgatttt ttgcagaaaa aaaaagagct cggggtaagc tctttttttc caccgaatta
    13501 atttccgtta ctaatccgat ttttaataaa tataaaatat atccttatga aagggtattg
    13561 ggaatagata tattaactaa ctagggttac cagggggata accttaattt attataacaa
    13621 aatgcgacac aaaaatcaat attttttttg aaataaatta taaaagttat ttttataaca
    13681 agtaagctta taaattt
//
LOCUS       DAKBNN010000102        13588 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000102 DAKBNN010000000
VERSION     DAKBNN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.102"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..140)
                     /locus_tag="OUB02_002411"
     CDS             complement(<1..140)
                     /locus_tag="OUB02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCT8886162.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AI"
     gene            complement(247..1605)
                     /locus_tag="OUB02_002412"
     CDS             complement(247..1605)
                     /locus_tag="OUB02_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HCT8886163.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(2953..4044)
                     /locus_tag="OUB02_002413"
     CDS             complement(2953..4044)
                     /locus_tag="OUB02_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886164.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(4274..5359)
                     /locus_tag="OUB02_002414"
     CDS             complement(4274..5359)
                     /locus_tag="OUB02_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCT8886165.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(5395..6708)
                     /locus_tag="OUB02_002415"
     CDS             complement(5395..6708)
                     /locus_tag="OUB02_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCT8886166.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(6709..9000)
                     /locus_tag="OUB02_002416"
     CDS             complement(6709..9000)
                     /locus_tag="OUB02_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCT8886167.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(8990..9784)
                     /locus_tag="OUB02_002417"
     CDS             complement(8990..9784)
                     /locus_tag="OUB02_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCT8886168.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(9823..10269)
                     /locus_tag="OUB02_002418"
     CDS             complement(9823..10269)
                     /locus_tag="OUB02_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCT8886169.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(10341..11747)
                     /locus_tag="OUB02_002419"
     CDS             complement(10341..11747)
                     /locus_tag="OUB02_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886170.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(11765..13135)
                     /gene="hydA"
                     /locus_tag="OUB02_002420"
     CDS             complement(11765..13135)
                     /gene="hydA"
                     /locus_tag="OUB02_002420"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HCT8886171.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(13268..>13588)
                     /locus_tag="OUB02_002421"
     CDS             complement(13268..>13588)
                     /locus_tag="OUB02_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCT8886172.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFVGTEGYYGILKS
                     VYGK"
BASE COUNT         4095 a         2680 c         1425 g         5388 t
ORIGIN      
        1 tctatcgctg ttcctatatt tttattactt aattttataa atccacatcc acctaaaata
       61 acattatttt ttttagaaat taaaatttta tatgcttctt ctaaactctg tggaactgaa
      121 tattccttta tagtaaccat cttatccccc catcattaac ttattaaaac acaaaagttt
      181 ttaataaatc ttttatttat atttataatt gtagtatatt taattcacat atattaaacc
      241 tttacattat aaacttttat ttttatcctc aaccttatca cttttatttc caggaaattt
      301 taccatatat aaagcttgaa ttattatggc taccattgct cctgaaacta cacttgaatc
      361 tgcaagtaat gtctctataa attttggaaa tttttcaaga acttgaggag ctaccatgaa
      421 tccaatacca gaagccatag aaagacctaa tatcatgcta ttttcttgat taaatccatc
      481 catactaaca agtcttaaac cagatgttgt aagagttgaa aatataacga gtgttgctcc
      541 tccaaccaca cagggaggaa ttgttcttat taaattagct aataaaggcg atattcctgc
      601 caaaaataat ccaattcctc caatagctag tacaaattta cttacaacct ttgtattgga
      661 aacgattgca gaattttgtc caaacatccc cattggagtt gcagaaaata atgcacctat
      721 tgtagagagt actccatttc ctaaaacagc agaagaaagt tcttcatctg ttacttgtct
      781 attaagccct cctaccgcag aaagtgtaca agcacccatt atatcagcta tctcaactaa
      841 atatattatt gaaaacatta caacaacttc aagtcttata tctagcccaa aagcaagtgg
      901 ttttggaagt gcaaccaaag taaactcctg aattgaagaa aaatttacta tacccaaaat
      961 taatgataaa atgtatccaa aaattatgct aactaatatt gatgcatctt taaataatcc
     1021 ttttccatac ttatttaatc ctaatatcat aagagataca gatattccaa ctataaaatt
     1081 aattggattc ccaaaagtct cattgcccat acccccagct aagtttttta tagctattgg
     1141 aaaaagtcca agacccatac atgcaactat tgttcctgat acagctggag taaatataaa
     1201 tcgtattttt ttaagaccaa aaccaattat aattattact ataccagcta ttatttgaga
     1261 gccaaataat actggtagtc catacttact tgcaacagat accccagctc ctataaacat
     1321 agctgtagct cccattataa ctggtattcc tcctcctaat ttaaaacctt taaacaaagg
     1381 tattggatat aattgtaata aagttgataa tcctgcaaaa actaatgcgg cttgaatcaa
     1441 taatgttgac atcttatgat taagtccagc tgcatttgca atcaatatcg ctggtaaaca
     1501 actacctatt accattgcca ttaagtgttg taaccctaaa ggtattgctt cctttagtgg
     1561 tggtattccg tctaattcaa attttgatgt actttttgct ttcataataa aaactcccgt
     1621 tacataaaac ttattgaatt atataaatac ttaaaaatct aatatattaa attaatgatg
     1681 ttcataaata tcaactatac tccaacatat atttaagttt ttagtcttaa aaaacagatg
     1741 caataaattt aattattaac aaataaacgt attctttaat tttgtataca ttatatacca
     1801 tgctttacaa atataagagt attatagcta tgaattttta attttttata gtaaatttaa
     1861 ttcaatttaa atctttcaat cttaatcatt ataagttata agttttcata agtatttaca
     1921 tgtttcaagt tttaaacatt tacctataaa cgctacttta tagatgtcac ttcaaatcca
     1981 gtgacctcat actttcatat gagcgtagac tatttcttca tcttcagcat tacctgctaa
     2041 gagcaacccg tttccactat caatcgctta tagtgtactc tctctcgaga tagtcgtttg
     2101 accttcctat ttctaggctt ggcgaccaaa tgtccatttt taagtactta ggctctcacc
     2161 atataccatc ctcactattg tttctggttt tcaccacatt cataaaagtt tctttctatc
     2221 ttttattgta gttgcaaggc tttaggattt actggtttta aagttgtgtc ctacatagat
     2281 ttctctatgt acggggccaa aagtacaatt tatttaacat actttgcttt accccctgta
     2341 aataatatta tttactcttt cactgttttt gttattaaat taattaaata tcaattaatc
     2401 taagcataaa ataattattt aatttaaact caaaatattt atgaatattt tataatataa
     2461 ttctcataag acagaaaaac ttaataagct atgtatctca gttgtaccaa tgtactctcc
     2521 tttactatat ttgtatctgc aacataaaat atacaagttt ctatctattt atagatatat
     2581 atatctataa gcaaaatagt aatattattt ttaatagtaa aaattttatt catattacta
     2641 tttataattt ttatagagca attttagtgc caatatttgc atacatttgt atagtataaa
     2701 atttttatgt tctcattgat aaaattttac ttatttcaag cgttctatta gattttatca
     2761 tctaaatttt aggttttatt tttataaatt aattgtattt acataaaaaa aaagacctaa
     2821 aaacaaattt ttaacggtct tttctcaatt ttttattttc atttctaaaa tttataataa
     2881 atagtaagaa cactataaat tcttttatat aaaattctta gtttgtggat gataaattag
     2941 tgaattaaaa tactacttta ctgattttag tttgacaata tcactccaat ttttactagt
     3001 ggctaactct actaaattta agtctcttct tatttcagct atatcctctt ttatagaatt
     3061 agtgtcacct tttatatatg ctatgttatg ttctatatta tcatgagtag ctttatttat
     3121 ttcagaatta tgtaataaac ttcctaaaat attattctgt tctatctgta tctctttcat
     3181 tgatttatct tcacattcta gactatcaat cttaatttca attccatata atttttcatt
     3241 tgctatggat acctcgtcct taattccatt catatcttca ttaatgttat ttattttatt
     3301 ttgcataaag tttacttctg atttaacttc ggaaacatct tctttcatta cagttatttc
     3361 ttgtctcact tctgacatgt cttttttcat tacatttatc tcttgtttta cctctgatgt
     3421 atcttctttc attacagtca tttcttgtct tacttcggac atatcttctt tcattatatt
     3481 tatctcttgt cttacttcgg acatatcttc ttttaataca tttatctcct gttttacttc
     3541 cgacatatct tctttcatta cagtcatttc ttgtcttact tccgacatat cctctttcat
     3601 tgtatttatc tcttgtctta cctcggacat atcctctttc attatattta tctcttgtct
     3661 tacctcggac atatcctctt tcattatatt tatctcttgt cttacctcgg acatatcttc
     3721 tttcattaca atcatttcct gttttacttc tgacatatct ccttttaata catttatttc
     3781 ctgttttact tccgacatat cttctttcat tacagtcatt tcttgtttca tttccgacat
     3841 atcttctttt aatacattta tttcctgttt tacttccgac atgtcttctt tcattacagt
     3901 catttcttgt tttacttccg acatatcttc ttttaacaca tttatttctt gttttacttc
     3961 cgacatatca ttttttatgc taactacatc ttcatttata gtatataata actctaaaat
     4021 cttctcttgc acttgcatca ccatccctat taattattat aacattttac acaaatgcta
     4081 tacaatgtta acatatatat cagaattatt aaaattttaa ttaaacaaaa ttgattcact
     4141 ttattacttc aaaactaatt aatatctaaa ataactacaa ataaacaaaa ataaggtcaa
     4201 caacaaaaaa ggcttatctc tttgagatag cctttcttat ttttatttaa aactttgttt
     4261 tttataaaat aatctataca ttcagctctg aacatgaaat ttttcttcca agtgcttttg
     4321 gtttacaaac tgatatacaa agtccacaat atctacactc ttcttcattc aattccatct
     4381 tatcattcaa ttttctcgct tgatatgcac aaactctctc gcacatctta catttagtac
     4441 atttttcttc atcataagta aactcaaagc taagtctttc attaacatct cccatatgat
     4501 tatgtactat accacaagca ttttctatag tattatatcc taatctatta agattagatt
     4561 ttaaatcatc atgtatttta gatattactt gtgcaccttt taaaattact actgaacata
     4621 taccaacaaa gtcagcacct gccataatca tttctaaggc atcatctcca cttacacatc
     4681 cacctaagcc aattattggt atattaactt gtttttttat ttcatatatt ctctgtaaag
     4741 ttattggctt gatagtttct ccacttaaat atcctattcc atttccacct aataaaggtt
     4801 gccctgtttc tatgtctatt ctaaatgctg gtcctacaga gtcacaagca gttattgcat
     4861 ctgctcctgc ttcttctaat tttttagcaa aatcgcctat ctcatctatc ataggaggaa
     4921 gtttaactat tacaggtata tttactcttt cctttgcatc tttaagcatt ggtatcaaat
     4981 ctctatagtc ataagatact aattcaataa agtcagctcc tgcatttgca actttctcta
     5041 ctaattcgct actttcttca attgtatgac ctattgaagc tatacagaca gtcccatctc
     5101 ttttcatttg tggtatttca aaatctatcc attgctctgg ctcaaaatct gtccattttt
     5161 cattatttaa aagagcatta gctgtatttc taaccatatg tggtgctgga tttatagctc
     5221 tttcttttct tatagtctta gtcacaactg cccctgctcc tgcatcactt aatatcttgc
     5281 atccagctgc actatatgtt aatggtcctg aacctattat aagtggtgat tttaattctt
     5341 tacctaaaaa attatacatt atctgcttcc ccttttcata tatatttaca ttcgttaaaa
     5401 tttccaaaat tcacaagcaa cttctttcat ttctttaaca atagcttttt tatctaaatc
     5461 aactaactgt ttttctttta aaatatgtct accatctatg tatacatttg atataaattc
     5521 ttttttaccc tgaactacta tctggtcaaa tatattatca agagtcacag gagttttaaa
     5581 ttcatcttcc ataactatta tatcagcctt attaccaact gctattttac cagaattttg
     5641 aagtcctaat acataagcac catgctctgt tgccattcta aatactaaat ttgctggcat
     5701 tactgatgca tcttcttcaa ctgatttatg cattaaaaat gcacctctca taacttcaaa
     5761 aaaatcattt atatatccat cagtacccag tgccactttt atgccttttt taatcatttt
     5821 tggtactgga gaaaaacctc cacctacttc acagttacta agaggcatat gaaccacttt
     5881 tacgcctttt tcttttaata tatctatttc ttcatcattt acttttacac actgagatgc
     5941 taatacagtt tcatccaata tatctaaatc attatagtag tcaactgctt ttttgccaaa
     6001 gtgtttttca gcatagtttg gctcatatat actctcacat aaatgaaatt gcattggtgc
     6061 atttaatttt tttgcatctt ctttagcttt cttaataaat ctatcagaac atgtgaagct
     6121 tgtatgtgta cacattattc catttattaa tttactattt tctctactcc actttattaa
     6181 attagaattt tcatctaaac atctaagtcc attttcaaag cttattcttt cacaactttc
     6241 taaggataaa atcgctttca tgcctatttc ttctagtatt tttccttgct caattaaagt
     6301 tccttcttct gtatttggag cttctagggt atcacaaaaa gctaccacac cagattctat
     6361 aagttctatt gctgatgctt tagttgttgc cttcacttct tttaatccaa ttctattttc
     6421 tataaatggc caccaatagt catttaaaaa accttcaaag tcagtaaaat gtacatttgc
     6481 cggtattccc ctagaaagaa ctccatattg atgcatatga ccattgacga atcctggcat
     6541 tattatagca tctgatttat ctattatttc ttcaatattt ttatatttta tttcaacttc
     6601 ttcatttggc aatatatctt ttataatgtc attttctact acaacagcag aatttgtata
     6661 tatttcatca gcagaagata ttaagtattt acttttaata gcaatcattt atttacctcc
     6721 ctgatttaat tttttcatgg ccatataaac tttctcagaa gttataggaa gttctcttat
     6781 acgaactcct gtggcattgt atattgcatt tgctattgct ggtgcaggtg tatgaataac
     6841 tatctctcct aaagatttag ctccatatgg acctgttggc tcataattat ctacaaaatc
     6901 aacaattatg tttcctatat ctttttttgt tggaactttg tattgtaata gattagaagt
     6961 ttgaagcttt ccatcagaac catgtctagg gtcttcatac attgcaagcc ctataccttg
     7021 agttacacca ccttcaactt gtattctagc aagtgcagga ttcataacgg taccacaatc
     7081 tgtagaacaa gcataattta ttactttaaa ctcccctgtt gttttatcta actctatttc
     7141 agcaaatcct gctaagaatg gtttagcact ctcttgtata ccataagatt cagatgaata
     7201 taaaacttct tgatttccac caccaacaga ttcttttcct aattcactta acaaaacaaa
     7261 cttctctaca tcttcactat aacaaactct gtcttcatat agtattaaat catctattgg
     7321 taaatcgagt ttcttctgtg ctacttttaa tatcttttgt tttaaacttt cagcagattt
     7381 tattgtagca tttccagtta cataagtagt acaagaagcg tatgcaccag tatcatatgg
     7441 acacatatct gtatctcctg tataaacgga aattctatca atagatgtat tcaatgcttt
     7501 tgcagctatt tgtgcaagta ttgtgtcaga accagttcct aagtctgaag acccagaaaa
     7561 caacttataa gttccatctt catccatcct catatttact attgccatgt ctatgttggc
     7621 tataccagaa ccatgagttc ccacagacat tccaacagcc ctcaccttat tgttgccaat
     7681 atccctaaca gggtactttt catcccatcc aataagtttt ttacctctaa ttatacattc
     7741 atcaagtgca caacttctta ttggaaaatt cattattccg ccttcagttt gtttttttat
     7801 aatattcttt aactttattt ctgttgggtc catattaagt ttatgcgcta attcatcaat
     7861 tgcacaatca agtgcaaatg taccttgtgt tgctccatat cctcttaatg ctcctcctgg
     7921 aactaaatta gtatatacag ttctcccatc aaattttatt gcaggtacat tattatatgt
     7981 tggaagtaca ttatgacctg attcagagca aactgaaggt ccattatcac cataagcgcc
     8041 tgtattgttc aatgctttca tatctatagc ttttatgttt ccttctttat ctgccccaat
     8101 ttttatatca aaaaacatct gatgtctagt attagtcatt gcaaaagttt cttctctagt
     8161 ataaattagt tttgctggtc tttttgtcat ccaagttaca aatgatgcat atatctctgt
     8221 tacagctatg ttttttccac caaaacctcc acctattctt ggttttatta ctctcacttt
     8281 tgaaagaggt attcccagtg ctttagaaac ctgtcttctc atatgataag ctgattggtt
     8341 tgcagaagtt actactaatc tgccattcgt atctatatac gtataagctc tatgagtttc
     8401 catcatacaa tgggcttgtg attgagtcat ataagaatgt tctactatta catcactttc
     8461 tttaaactct ttttctacat cccctttacc cattttttct ctcgatacaa tattttttgt
     8521 agcatccaat ccaaaatcaa atggacaaaa caaatcatct tcttcatgga ttaaaatttt
     8581 atttccttcc gattctcttg catccaatat aggagtcatt acttcatatt caacttttat
     8641 aagtttcata gctttaatag cagccctttc atcttcagcg gctattatag caacttcatc
     8701 tcctacatat ctaactacgt cttcaagtat taattggtca tgaggtgatg cttctggata
     8761 tgactctcca accattgaat atctataatt tggaacatct ttgtaagtga atatgtttac
     8821 tacacctttt acctttaatg cattttttat atctatgttt tttatcttcg caaaagcatg
     8881 tggacttcta agaagcttaa ttgttaaaac atctttatga aaaattaaat cttctgtata
     8941 tattggtttt ccagtaacaa tggcttcact atctattttt cttacctctt tatttaccat
     9001 cacactcacc tccaatacta ttatacattc tcactactaa ggcttttgcc atatcttttc
     9061 tgtattcttt acttcctctt atattacttc caaatgaaag ttcttcactt gatacttcac
     9121 cagctttttc tatatcattt tctaaactta atatttcact tgctttatag gcaatttttg
     9181 ctcttcttgg tctagcgcct attgcaattt tataaatact tccttctttt aacattgctc
     9241 catttaatac tgcaaaatca cctgtacatt ttcttataga atcaaataca cctatagcat
     9301 cttttttagg taatataact tctattaata tatctttttc aagctcactt tctaaaaaat
     9361 cagctaagtt cataacccct tttttatata acttaacctt tgcatctacc actagtaatg
     9421 aaggtattaa atctgaaaat ccatacttag caaatacgct agctcctact ctagctccac
     9481 ttctaaattg aactcctact atattagaaa cagcgtttga aattacccca ttacagtaat
     9541 ttttaataac cttatttaat tctaaagttc ttaaacttgt gtctgctcca attaatatgc
     9601 tatcatcagt ttcatttata taatctaaag ctaagtcttt taaatctatt gctgagccaa
     9661 tatttttact acctagtttt aaaaatccac atcctccaag tattatgttg ttttttcttg
     9721 atattagcag ttcataagct tcatctaaac tttttggaac tacatattcc tttatagtta
     9781 tcatactctc tccccatatc aacttaattt tcacttaaaa gtctaagtgt ttaaatattt
     9841 atgtattgct cttaattgac ctttatatcc tgtacatcta caaaggttac catttacata
     9901 atctataact tcttcttctg ttggattttg aagctctctt ttcattgcca aaaccatcat
     9961 tataaatcca ggagcacaaa atccacactg gtcagcccct tcgtctgcaa gaaaatctcc
    10021 aaacttcttt gcttcatcca agacaccctc aagtgttgta atatgctgac cgtctactct
    10081 agcagtaaga taattacatg aaagcacggc cttgtcattt accatcactg tacataaccc
    10141 acatgagcct gtatcacacc cttttttaac acttacatat ccattttctc ttaatgtatc
    10201 tgctaaaaaa tcatctggtc ttatacttaa ttctttttct ttattattta tggtacattt
    10261 gataatcaaa taaatcacct caaaactcat atttttaagc taaagtttta ctatactttt
    10321 ctatgaataa attacattta ttaataaagg ctattccatt tatgaccttc cattctattt
    10381 tttatagttc ctcttacaca aagtctctca gctgtatctt gagcatcttt taatgctttc
    10441 tcttcattag ctgtcaatat ttttgaatct tccattatta tatttccatc tactataaca
    10501 ctctctatat ttttcatact agaagaatat actaatgtag ataccggatt atgcataggt
    10561 atcgcttttg gtgaaagcat tggattaaat attagtaagt ctgctttttt acctatctcc
    10621 aatgaaccta tctcatcttc cattccaata gctcttgctc catctatagt tgccaactca
    10681 agaacttttt ctgctgacat tgctaatggg tcacacttat taactttatg ttgaagtgct
    10741 gttaatttca taagttcaag catatcttga gagttattac tagcggctcc atctactcct
    10801 aaactaactg ttattccttt tttaagcatt tctggtactg gtgcaacacc tgaagataag
    10861 tacatattac ttgctgtatt atgagacact ttcatatcgt attttttagt aagttccata
    10921 tctttttcag ttagatacac acaatgtacc ataagcacat ttggtccaag tattcctaat
    10981 ttttctaaaa catctatgtc atattgccca tgcaattctt ttgctgcttc tctatcaaaa
    11041 ggagtctctg atatatgtac tgtaaataaa gcatcatcat attcttttac tactctccat
    11101 agcatttcaa gcatttcttg agaatttgac catattgctg caggagctac tcctattttg
    11161 attcttccat tttctgtatt atggtgtttt tcaaacaatc ttcttacatc tttttcaacc
    11221 gtttctacat cttgcattat tccaggatga actccaaatt gagctcctgt attcatacat
    11281 cctcttccaa gtattcctct tattcctaat tctttataag catctattat tccatcacaa
    11341 agacctggct tactatgagg atacatgtag tcaaccattg ttgttatacc acttctaagt
    11401 ccttcaatac atccaagcat tgcagcatca taagtatctt ttggctctaa atagttagct
    11461 gctggaaatg tcatagtttc taaccagtct ttaagtacca tatcatctcc caatcctttt
    11521 aataaagttt ggaataaatg attatgtgta tttataaaac ctgggaataa aactttacct
    11581 ttagcatcta taattttttt aacatcagta tatttacttt ctatctcttt agaatttcca
    11641 atatcagcaa tcttgttatc ttttactact aatgccccat caaaaataac ttctctctct
    11701 ttattcacag ttactattat ggcattctta ataagtatat ccatcattct tatttccccc
    11761 cgcattattt agtaattttt cttttaatat attgacctgt tggatgttct ttaacattac
    11821 aactttgtac tacatgattt cctctcaaaa atacatcttc aactttacat ttaaccttaa
    11881 atccttcata tggagtataa tctacatttt gtttttgagt tttataacta attgtttctt
    11941 ctttattgat atttaaaatg actatatctg catcacttcc tacagctatc tcaccttttt
    12001 taggatacat tccaaatatt tttgcagcat tcgtagaagt aacttccaca aacttattta
    12061 cacttattct attttctaag accccatatg tatataaaag tgctaaccta tgttcaacac
    12121 caggtgctcc atttggtatc ttactgaaat catcaattcc caagtctttt tgccccttaa
    12181 aattaaatga acagtggtct gtgccaactg tttgaatatc tccactagac aatccttccc
    12241 aaaggtaatt tatgtcttct ttttttctta aaggaggaga cattacatac ttagcaccct
    12301 caaatttatc cttattatat aaattgtctt ctaataatag atactgtggg catgtttcta
    12361 ctatcatatc tatatttttt tctctagcca tttttacagt ttcaagtgac tccctacaac
    12421 ttaagtggac tacataggat tttgaattag ataatttagt tatatcagca agtcttgaaa
    12481 cagactcttt ctcaactatg ttaggtcttg taagtggatg atattttggc tctaaattac
    12541 cattttgtat attttcttct atcaatacat ctagtaaatc cccattttca caatgaaatc
    12601 caactataca acctaaatcc cttgctttct ttaaaaccct gtatattgtt ccatcatcta
    12661 ctttcatgcc atcatatgcc atatacatct taaaagatac tatcccatct gaaataagtt
    12721 tttccatatg attaaatgta tctttattta aacatgtaat tgtcatatga aaaccataat
    12781 cacaataaca atttccactt gcttttttat gatatgcttc tacaccttgc agtaggtttt
    12841 ccccctcatt ggcttctgca aaatctagta ttgtagtagt tcctccagct atagctgctc
    12901 ttgttcctgt ttcaaaatta tctgcagttg taatagaacc tgcattcata tcaaaatgag
    12961 tatgtgtgtc tattccacca ggaattacaa ttttacctgt agcatcaata accttatcac
    13021 catttatttc taagttattt cctatttcaa ctatcttttc atcttctatc cttaaatcac
    13081 ttatataagt cttatcagag gtaattatag ttccacctct tagtattgtt cccatatata
    13141 cctcctaact tttaagcttt aaagtatttt ctaagtattg taaaacttta tttaaaattg
    13201 tattttctaa aggtgctata ttcagcacct ctagactata tttaaataat ttattgtgaa
    13261 ctaaatatta ttttccgtat acagatttta atattccata gtaaccttct gttcctacaa
    13321 ataactgttc ttgctctata tactcatcta ttgtgtgagc aagattttcc ttagaaggac
    13381 caaatcctat agtttttatt ccagcttcac ctgcatagtg acttccattc gtacagaatg
    13441 agtattgagt tatttctgaa tctattccag cttcttttaa tcccttatat gctgcttgaa
    13501 caaattcatc ttcttcgtca tataaccaac ctgggaagaa tctttcactt tctatagtat
    13561 ttcctgtata gcaatcagct ttttcaac
//
LOCUS       DAKBNN010000103        13515 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000103 DAKBNN010000000
VERSION     DAKBNN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.103"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            98..2599
                     /locus_tag="OUB02_002422"
     CDS             98..2599
                     /locus_tag="OUB02_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCT8886173.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAEAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2759..3427
                     /locus_tag="OUB02_002423"
     CDS             2759..3427
                     /locus_tag="OUB02_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886174.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3411..4688
                     /locus_tag="OUB02_002424"
     CDS             3411..4688
                     /locus_tag="OUB02_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCT8886175.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5089..5310
                     /locus_tag="OUB02_002425"
     CDS             5089..5310
                     /locus_tag="OUB02_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886176.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EIKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5599..6363)
                     /locus_tag="OUB02_002426"
     CDS             complement(5599..6363)
                     /locus_tag="OUB02_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCT8886177.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6637..7311
                     /gene="fsa"
                     /locus_tag="OUB02_002427"
     CDS             6637..7311
                     /gene="fsa"
                     /locus_tag="OUB02_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCT8886178.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7433..8827)
                     /locus_tag="OUB02_002428"
     CDS             complement(7433..8827)
                     /locus_tag="OUB02_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCT8886179.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLET"
     gene            complement(8905..10251)
                     /locus_tag="OUB02_002429"
     CDS             complement(8905..10251)
                     /locus_tag="OUB02_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8886180.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      10501..10603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10697..12073
                     /locus_tag="OUB02_002430"
     CDS             10697..12073
                     /locus_tag="OUB02_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCT8886181.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(12187..13332)
                     /locus_tag="OUB02_002431"
     CDS             complement(12187..13332)
                     /locus_tag="OUB02_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT8886182.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
BASE COUNT         5206 a         1625 c         2097 g         4587 t
ORIGIN      
        1 atatagattt ctaaataagt aatttatcaa tattacatat caaaataaaa cgttacaaat
       61 tagctgaaaa tatattgaaa gaaaggaaga ttttattatg agttcaaata aagtggtaga
      121 aagttataaa attacaggta tgacatgtgc agcttgtgca aaggcagtag agagagtaac
      181 taagaagatg gatggagtat atgaccaaag tgtaaatata gctactgaaa aacttaaaat
      241 agaatatgat aattctaagg ttaattttga tgatataaag caggttgtag aaaaagcagg
      301 atatggaatt ataaaggaag aaagtaataa aaaaattgat atgaaaattg atggaatgac
      361 atgtgcagct tgtgcaaaag ctgtagagag agtggtaaaa aaattggatg gagtagaaag
      421 tattagtgtg aatattgcaa cagataaagc taatatagac tatgacccat caaaagtaaa
      481 attatctcaa ataaaagcag ctattgaaaa agctggatat aaaccaatag aagaggtaag
      541 aaataaggtt gatgtggatg aagataaatt gagaaaagaa agagaaatga agtctttatt
      601 tgttaaattt atagttgcaa tagtatttgc agtacctctg ttttatatag caatgggacc
      661 tatgataata aagcctatag ggccatggcc tttgccagaa attataaatc ctatgacaaa
      721 tacttttaat tatgcattaa ttcagttaat tttagtaata ccagtaatga tagctggtta
      781 taaattttat ataaatgggt ttaaatcact tttttcttta agccctaata tggattcttt
      841 agttgcaata ggaacattag ccgcattttt atatagtcta tatacgacac tacaaatagc
      901 caatggacaa attcaaggta tgcaccatca tcagttgtat tatgaaagtg caggaataat
      961 aatagcactt atcttacttg gtaaatattt agaatcaaaa tcaaaaggaa aaacatcaga
     1021 agcaattaaa aagcttatgg ggctacaacc taaaactgca atagtgttag ttgatggtaa
     1081 agaagttgaa actcctatag aagaagtaga gataggagat atactattag taaaaccagg
     1141 aactaaaata cccgtggatg gagttgtaat agaaggatat acatcagttg atgaatcaat
     1201 gcttacaggt gaaagtatac cagtagaaaa gaatgttgga agtaaggtga caggagctag
     1261 tattaataaa aatggtgtta ttaagtttaa ggctgaaaaa attggtggag atactgctct
     1321 tgcccaaata ataaagttag ttgaagatgc acaaggtaca aaagcaccta ttgctaaact
     1381 tgccgatact gtatctggat attttgtacc aatagtaatt gcaatagctg tagttgcatc
     1441 attattgtgg tttttaatag gaggaaaaga tatagtattt gtacttacta tatttatatc
     1501 agtacttgta attgcttgtc cttgtgcatt aggattagca acaccaactg ctataatggt
     1561 tgggactgga aagggcgcag aaaatggaat cttgataaaa ggtggagagg cactagaatc
     1621 tgcacataaa gtaaatacag tgatatttga taaaacaggt actataacag aaggaaagcc
     1681 aaaagtaaca gatatagtat taaataataa tgtaaaagaa gaatatctta tcaagattgc
     1741 ttcaagtgca gaaaaaggtt cagagcatcc tcttggagaa gcaatagtta agtatggaga
     1801 agagaagaat ataaaatttg aaaaagttga taattttaaa gccataccag gtgctggtat
     1861 acaagttact attaatgatg aaagtatatt acttggaaat agaaagctta tgaatgacaa
     1921 taatataaag cttggggatt tagaagaaaa atcaaatata ttggcaagcc aaggtaaaac
     1981 acctatgtat atagctgtag atggaaattt atcaggtata atagcagttg cagatgtagt
     2041 caaagaaagt agtaaaaaag caatagaaat acttcatgat atgggaataa aagttgcaat
     2101 ggtaactgga gataatgcga aaactgcaaa tgctatagca aatcaagtag gtatagatat
     2161 ggttttggca gaggttttac cagaagataa gtcaaaagaa gttgaaaaac tacaaaatca
     2221 aggaaagttt gttgcaatgg taggggatgg aataaatgat gccccagctc ttgcaaaagc
     2281 agatataggg atagcaatag gaagtggaac ggatgtagcc attgaatctg cggatatagt
     2341 acttatgaag agtgatttaa ttgatgttcc aacagcaatt aaacttagtc atgaaactat
     2401 aaaaaatata aaacaaaacc tattttgggc atttggatat aatacaattg gtatacctgt
     2461 agcagcagga atattatatg tatttggagg acctttatta aacccaatga ttgcagcaga
     2521 agcaatgagc ttaagttcag tttcagtcgt atcaaatgca cttagactta aaaacttcaa
     2581 ggcatataag agagattaga ttaataaatt aaataaaatg tatccaaaag agctatcttt
     2641 aaattaaaag gattaattta caaattaaga tagctctttt ttcatgtaaa attcacaaca
     2701 ataagataaa atataagtaa tatacgaaat tttaaataaa atagaaagga ttgatagaat
     2761 gaattcgtca atacttgtaa ttgaagatga ttcaaatata caagagttaa tatcagaatt
     2821 tttaagtgca gaaggttatc aagttgatac agcaaatgat gggttggaag gtatacaaaa
     2881 gtttaagcaa ggaagttatg atttagtcat attagatata atgatgccaa acttagatgg
     2941 ttatggagtt tgtaaaatga taagaaagtc atcgagcgtt ccaattatat ttttaactgc
     3001 tttaaatgat gaaggagacc aacttaaagg gtttgactta gaatgtgatg attatataac
     3061 aaaaccattt tcatttaatc ttcttataaa aagagtagaa gcaattttaa ggcgaagtaa
     3121 caagactata aatgacaaat ttatagtttt tgaaaaatta aaattagatt taaacacata
     3181 tattgcagag atagatggag aacctataga acttacacta aaagagttta atatattaaa
     3241 agctttgata gaaaagtatc cacaagttat aactagggaa ggtcttttgg atagtatatg
     3301 gggttatgac tattatggag atactaggat tgttgatgca catataaaaa atataagaaa
     3361 aaaaatatca ttaccatata ttaaaactgt aaaaggaata ggatatacac ttgagaagga
     3421 tatttgataa gtgggaaaag ttaagtataa agtataagtt attttcaata acgacatcat
     3481 tattaatagc attagcttta ataatatatc taattttata ttttttattg ccatcatatt
     3541 atcatgagta taaaattgag tctcttcaag aaagtttgaa atctttagta gatagttcaa
     3601 ttcatttcga tacatatact ttagaagaaa gactttatta tatggctaaa gaccaaaatt
     3661 tggctatttt attaaaagat aaccaaggta aaattgtata tggtaagaat gaagtagtta
     3721 ttttaagata tagtaagtac atgattaata gtcttgagga tgagtataga acttctatac
     3781 ctatatatac taaagatgca aaagatggtc catatacttt agaactcgtt atgccattac
     3841 aaccaattga cgaagcaaac gaagttataa gaaaactaat gccatacatc atatcaatag
     3901 caatattgat agcgattatt ggagcatata tatattcaat tgtaataaca aagcctctta
     3961 taaacataat agagagtgag agagaacagg aatatagaag gaaggatttt gttgctacaa
     4021 tatcacatga actaaaaact ccaataacca taataagtgg tcaaattgaa ggtatgattt
     4081 atagtgtagg taagtataag gatagggata cttatctgaa aaaatcgtat gagtgtacac
     4141 aagaattaaa agatttagta aatgaaatga tagaggtttc aaaatcagaa atacttgaaa
     4201 aagatttgaa attggtatca ataaatataa gtgaattatt aaatagactt gtaaagagac
     4261 aagtattttt aatagaagaa aaacatatga aaactatttt aaaaatagaa gaaaatttgg
     4321 aaattaaggc agaccaagag agaataacta aggcaatcaa taatataata aataatgcca
     4381 taaaatattc tcctgaagaa tcagagctta taattagact ctatgataaa aataaacgaa
     4441 taagtaaaaa gacttctaat caaagagtgg ttttagaaat tgaaaatact ggtgtaacta
     4501 tcgagaaacg ttatttagaa gaaatattta atccatttta tagaatagaa aaatctcgtt
     4561 caagaaaaac tggaggaagt ggacttggac tttatatagt cagccaaatt tttaaaagtc
     4621 atgggtttga ttatagcata aaaaacaaag aaaactctgt agtattcaca gtagaattta
     4681 aaaattaatt acttattata aagaatatat aactttcaat aatagttcat tttaaaatca
     4741 caaaaaattc ataatgaaac tgtattatat aaatataaag aagcttaaaa ccataaaata
     4801 caccaactat aatgatgaga tttccttaca tcctccatat aagtcatcat tatagtaacc
     4861 caacaattaa gttactatga atttaaaaaa taacaccgag catataatct atgcttagtg
     4921 ttatttttat tttgaaataa aaaatattta atttattgaa atgtatagat ttagatagtt
     4981 taaactatat ttaagactta aaaattataa ttgatattac aaataaatat atgtactttg
     5041 ggaaaaataa atataggaag tattatattt acagaggagg tagttttgat ggatagatta
     5101 aaaagagata tgaaaaatat gaaagaagat ttaaaagaag gtgttaaaaa gacagcttat
     5161 gttatgaaaa atacagctga agatttgaaa gatgatgcac aaggtgctat ggtaaactta
     5221 gaaataaaaa aagatgaaat gatggaaaga tatgaacaaa agaaattttc taaagaatta
     5281 gagaagcaaa tgaagaatga aatgaaataa gcttttattt atgaataaca aattattgta
     5341 ttcatataaa aagtagccag taatattaaa tagtttattt aaggaaatag actttctaaa
     5401 aataactgta ttagatttag aaagtctatt tcaaattagt tttataaata gacataagga
     5461 taaagtagat gttatagaac tagattttaa aaaatagata ctaaggataa aataaatatt
     5521 atagaaatat gtttttaaga atatttatca aataaaaatt tatatttata agaaatccta
     5581 taaaacatat aattaaactt ataaaacatt tacattaaat tctgataaaa tatctactat
     5641 atcaggagta ggcgtttttt ctgtaattat agaactaata tcttctaaat gagcaaactt
     5701 gaaatttcca tcaaatttaa actttttatc ttccataact ataaatactt ttttacttga
     5761 atttattata gccttcttcg taagaccatc ttcaatttca aaagttgtaa gtgaagagtt
     5821 gaatacatct actccacaac ttcccataaa agtcttatca aacttatatt tcgatataaa
     5881 ctctatagtt ggagtaccga caaaaccgtc taaactgaga tttaataaac ctccagtgga
     5941 aattatattt aaattatttg gttttgaagt aactacattt agtatatcca gcatatttgt
     6001 aacaagtgtt acttttttat tactttcagc taacaacttc gcaagcaaga tgttaattgt
     6061 agagatatct aaaaatatag tttctctatc ttcaattaaa ttaaatgctt tttctgctat
     6121 tattttcttt gttggaatat taatttcttt tctgtctttt gtatcctgat tttctagtgt
     6181 ttctctagct agtacagctc ctccataaat tcttcttatt gaagaaatct tttctatatt
     6241 ttttaaatct tttcttatac aatcttctgt aacattaaat ttagaactga gctctttgac
     6301 agtaactcta ccttcttttt ttaggatatt taatatagct tggattcttt cttctgcaaa
     6361 cattttctac ctcttttcgc aatatagtat tatagtaaat attataagat aaaatacaat
     6421 atatcaacta ttttaacatg aaatacaaaa ttaatttaat ataaaaagta tgttttttct
     6481 aactatatat ttagaattta gtaaataaaa aaattataaa actaagaagg aaaagtaaaa
     6541 caataataaa aaataaaaaa caataagcaa aatgttgaaa ataataccaa atcaatatat
     6601 aataagaata aagaaagatt aaagggggat attttaatgc aactaatctt agatacaggt
     6661 aatgtggagg agattaagga gttatgtact tgtttaccaa tagatggtgt aactacaaat
     6721 ccaagtattg tttcaaaaga aaaaaagaat tttaagcaac ttataaatga aattggtgag
     6781 attataggtg aggatatgcc tatacatgct caagtgttaa gtacaaagta tgaagaaata
     6841 ttagaagaag cattatatat aagtagtttg agaaaaaata tatatgtaaa aatacctgta
     6901 acacaagatg gtttaagagc tataaaagac cttcacagaa aaggtgtaaa aataactgct
     6961 actgcaatat tcactgctca tcaaggtttt ttagctgcaa aggctggagc aaattatatt
     7021 gcaccttatg taaataggct tgataatata tctggtgatg gagtagctat ggtttcagaa
     7081 cttataaaaa taatagatac ttataaaatg gatacaaaag tacttgcagc cagttttaaa
     7141 aatgcacaac aagttataga attaatgcaa catggggtgc atagtgttac agtaccatat
     7201 gatatttgta aatctatgat gaatcatcca ttaactgatt ggagtgtaga taagtttata
     7261 gaggattggg agaatacttt tggaaaagga agtaaaacaa ataatattta gtattatggt
     7321 aaaagagtag ttttgtccaa tttcacaaga acttatataa aaagatgaaa atatgaattt
     7381 tctttttaat aatttcgtta ttatcttaag ttaattcata ttttcatctt ttttaggttt
     7441 ctagtcctat aaatttccac caagggaatg ttatacatat tattataaag taggttaaag
     7501 gaatcaaagt taaaccgtat ttgaaaataa ccttgctatt atacagattt tcaccatgac
     7561 caaccattat tgtagcatga tgaaatggta atatgtactg tatgcttact ataatgtaga
     7621 caaatagaca taaagtaaca ctattttcct ttagtatatg taaacttcca agtaatggta
     7681 ttactacaga tgatgtagtg acagcacttc caagacatat attcaagatt attgtgagag
     7741 ttatcaaaaa caatattgaa ataatttctg aagagtttgg tataaacttt atcaaataat
     7801 ttcccattat atctacaact ccagagtact ttaacactcc tccaattgaa aatgctgcta
     7861 taaaaaaaat aaggaccttc caatttattt cttttaaagt actaaatcca actatttttc
     7921 ttagtaataa aagaagtaca caaattgaca ttataattaa tgtgtttatg ttatgtatga
     7981 actgagttaa aaacataaaa aacattattc ctaggcataa aaatacgtgt ttttgttctt
     8041 tgtttagcaa attaaattct ttttttaaat tagaatatga aaaatgaagt ttattaccct
     8101 caagattttc attagaaata aattttattg aatgcaattc ttttttaaag acaaaggtaa
     8161 ataaaaaata agttataaaa caagtaatta tagttggaac agacatataa aaagcccatt
     8221 gaccccaatt tatattacaa tttccaagtt ctagtacaac ataatttaga agagtgtctc
     8281 catttacaaa aaacattgat gtaaaggtag atgctgtaaa tatactaaat aaaagtatag
     8341 attttgaatc ttcagttatc tgttgttctt ttaaaaactc cttgtaaaaa gcagatacaa
     8401 gaataaccct tggaaatggt tgtgggataa aaaatattaa aagtatacct aaaatatacg
     8461 ataaaagaat cattttttta ggggtattta cagtatttaa aattaacttc tcagaaataa
     8521 tttttgcgac acctgttttt gtaactgcat tagtaattat gtatgcaaga attattatgt
     8581 aaaaattttc tgtaaataaa aactgtaaaa taacctttat tggtgcaatg ttaaatataa
     8641 acgctagaat tacaaaaaac aatgcaacaa aagttttttc aactgagtct acagcccata
     8701 tcaagatagt taaaaaagat aagcatatag aaaattttat gttgaaactg taattaagag
     8761 agaagtagag aattatggta gctataacag tcatataaaa ttgttttggc ttaagaataa
     8821 tacccatttt tatattcacc tccataattt attatatcat aatggaggtg aatacaatga
     8881 atgtgaatac attttataaa aagattattc attgattcta tgatttacaa tagctttagt
     8941 tttccaatgt cctttcttat agtagataac tgtaacaata agacctaaag tccaaccaaa
     9001 aggaataccc atccaaattc cgtctgttcc aaaaaattta gaaagcatat aagaagctgg
     9061 aagtcttcca agccataaag atactattga gcttataagt gcaaacatgg aatcaccagc
     9121 acctttcatt gcacttgaaa gtacaaaaga agttccaata aataagaaga atggtcctat
     9181 tataaataag taagaagagc caattttaat gacatcttta tcagtattga atagagcaat
     9241 tagattttcc ctaaatagca aaattaaagc aataactgta actgctaaaa ctaaggtcat
     9301 ttttatagat tctcgaatgc cttttttaac tctttcattt tctccagcac ctatgttttg
     9361 agcaacaaaa gtagaaacag cagaaccaag attcataata ggtaagctta taaaattttc
     9421 tatcctaaga ccagcaccaa aagctgccat agcagatgta ccataactat taactaaaaa
     9481 ttgaagagcc atatttccaa ttgaaaacaa catttgttga actccacttg gaagtcctaa
     9541 tctaagtcca tctttaaata ttttgttatc atatacaatt cccttgattt taaatttaag
     9601 cacttcatgt tttttattta aataaattat acttattata aatgatactg cctgagccat
     9661 tatagtggca agtgcaacgc cctcaacacc cattcttagc acgacaataa aagttaagtc
     9721 taaaactata tttagaattg tggctattat cagaaaataa agtggagtta ctgaatctcc
     9781 aagtcctcta agtattgcac ttactgaatt atatccagca gaaaataata ttccactaaa
     9841 tattatagta agatacaatt ttgattgagc aaaaacggat tctggtgtat tcataagctt
     9901 aagcatgggg ccaccaaata ctattccaag aatacttatt aatatagaac caataaataa
     9961 aagtatatat gtagtatcaa tagtagcttt tacctttttt aaatctcctg agccataaaa
    10021 ttgtgacact aaaatagaaa atccaaggct aacacccata agcgctgcca ccaataaaaa
    10081 cattataggg ttagctccag agactgctgc cattgcttct tttccaataa atctaccaac
    10141 tattatactg tcagctgtat tgtatagttg ttgaaataaa ctaccaataa gcataggcat
    10201 ggcaaaaaag aatatggact ttccctcatg acctgtagtt aaatctttca ccttattcct
    10261 ccaaataaaa aaattattaa tttattatag tataatctta acataaactt tatatccaaa
    10321 ctataaataa attgtatcaa aatatcatat atttttttaa aatgtaaaga atataattat
    10381 aatttgaatt taataaatca atatttaata taatcgaata ttaatacaaa atcaatacaa
    10441 tatatacgta tatattgaca aaattaatta tataaattaa aataatattc gtaaagaaaa
    10501 attcatgttt tgtactgtat aacatataat tttgacaata tgggtcataa gtttctaccg
    10561 gaataccgta aatattctga ctatgtatac tattatttag ttaattaaat atttaattaa
    10621 ctgaattatg aaggatacgg ctcagcttat caactactgt gtcgtttttt tgtaaaattt
    10681 aataggaggt gttgttatgc aaacaacaaa cagtagtact atgcagaaac tttttccaat
    10741 tttgactaat aaaaatgtcg acatgaagaa ggaaataatt gcaggagtaa ctactttttt
    10801 aactatggct tatataatag ctgtaaatcc aaatatttta tctgagacag gcatgcctgc
    10861 aggtgcactt gttacaggta cttgtttagc agcagcattt ggatgtattt taatgggtgt
    10921 agttgctaac cttccttttg ctttagcatc aggtatgggt cttaatgcat tttttgcata
    10981 tacagtagtt ttacaaatgg gtgttccttg ggaagtagct cttacagcag tatttgtaga
    11041 aggtattatt tttatagtat tatcagtaag tggagttcgt gaggctgttg taaatgcgat
    11101 accaaaaaat atgaaattag ctgttacagg tggtatagga atatttattg cacttatagg
    11161 tctagttaac agtggaattg ttattggaga ccaagccact cttatcaaaa tgggtagatt
    11221 tacaccagca gtaataataa cttgtgtagg attaataatt attgctgtat tagataaaaa
    11281 aagagttaaa ggttctatac tttttggaat agtagtgagt tcattacttg cttgggcatt
    11341 tgcttttata aatccagagt atgcacaaaa attaggtata tatttacctg gtggattatt
    11401 taaattcgaa agtttagcac caatagcagg taaaatagat ttaggatatg tactacatcc
    11461 aacaaatata ggtggttttt tagtagtagt ttgtacattt ttatttgttg atttttttga
    11521 tacagtaggt acattagttg gtgtttgttc taaagcaaac atgttagatg aaaaagggaa
    11581 tgttcctaat gtaggtagag ccttacttac agatgcggtt gcaactacag ttggtgcagg
    11641 acttggagta tctacagtta caacttatgt tgagagttca acaggagtta tcgcaggtgg
    11701 aagaactgga tggacagcaa taacagttgg aattttattc ttagctgcaa tgtttttctc
    11761 tcctgtattt atagcaattc catcatgtgc tacagcacca gctttaatat atgttggata
    11821 tttaatgctt ggtacagtta aggatataga gtttgataat ataactgaag gtgttccagc
    11881 ttttgttact attgcttgta tggcattaac atatagtata ggtgatggtt taactttagg
    11941 tatattaact tatgttttcg taaatatatt ttataatata tttggagcta aaaaggcaga
    12001 agacaaaaaa catgtttctg tagtaatgat tgttctggca atattattta taataaaact
    12061 ttggctacta taataggtat tatttaaaat tttaatttaa tataaaataa agggctgtaa
    12121 tttattatca attgtgatga taaactacag ctcttttgta tttgttgtat ttttgtattt
    12181 attgtattat ttaataatgt ctattagttc tccattttgt aaagttttag gtgtaattac
    12241 tatattgaac tctttttgca aatcttttat aatatctaat tctttttttg ttattattga
    12301 gattgtgtta ccacttcgat ttcctctagc agttcgacca catctatgca ggtattcatt
    12361 tttacttttt ggaaaatcca agttaaaaac atgacttaca tccacaatat ctagtccacg
    12421 agcagataaa tcagttgtaa ttaaaatctt tgctttacca agtttaaatt tatttattgc
    12481 attctttcta tcttcttttt ccatatttcc aaaaattcct attgctttgt aattatgata
    12541 atttagtttt gatactagga cttctatatt tttttcatta tttacaaata caatagcttt
    12601 ttttggattt gtagctgcta atgcctttct aagaaaagta aatttatccc ttatctcacc
    12661 taataaatat gagtgattga tgttaggatt tatttgtgat ttttcctttg tttttattat
    12721 ttcaaactcc ttcataagtt tatcacaaat ttttatagtg ctatcagtaa gacttgcaga
    12781 gcatcctata atttgtctat ctcttaacgt tgttcttatt atgtcttcta tacaagttat
    12841 attttttcca ttcaatagat tgtccacttc atctaagata atagttttta tattatgtga
    12901 ttttagtttc ttttgtttta tcaagtctaa aactcttcca caagagccaa ttactatgtg
    12961 tggttttact gcttttatat tttttatttg tttttgtata ttgacctccc caattaaagc
    13021 taaagaagta acggagagtt ctgcattttt agccaataat tcaacctggt ttgtaatctg
    13081 cattactaat tcatgcgtag gtgcaagtat aagagcttgt gtttctcttt ttgatgtatc
    13141 aattttttca aatataggta aaaggtatgc tagtgttttt cctgttccag tctgtgaatt
    13201 aatcaataaa tccttatttt gaattatatt ccctataaca agtgactgaa cttctgttgg
    13261 agaagttatg tcttgttttt tgagtccatc tattagagtg gaacttattt ttaattgttc
    13321 aaatgtattc atattttact ccttatattt taataattat agtcaactat tttttattat
    13381 acattgttta ttcaaatcga actatatatt cttttataat tatgtgtaat ttgaaagtaa
    13441 atatcaatta tatttatacc tttatatttc tgataccttt gcattttata gatttctaaa
    13501 ataaacaaat aaaag
//
LOCUS       DAKBNN010000104        13462 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000104 DAKBNN010000000
VERSION     DAKBNN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.104"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            865..1527
                     /locus_tag="OUB02_002432"
     CDS             865..1527
                     /locus_tag="OUB02_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886183.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1536..4043
                     /locus_tag="OUB02_002433"
     CDS             1536..4043
                     /locus_tag="OUB02_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886184.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQIMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4374..4718
                     /locus_tag="OUB02_002434"
     CDS             4374..4718
                     /locus_tag="OUB02_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886185.1"
                     /translation="MYCLSVVLVIYGIYVFINCTEYIYTMLSTKQLTVTGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGSIENSILKK"
     gene            4956..5642
                     /locus_tag="OUB02_002435"
     CDS             4956..5642
                     /locus_tag="OUB02_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886186.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            5653..6894
                     /locus_tag="OUB02_002436"
     CDS             5653..6894
                     /locus_tag="OUB02_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8886187.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            6965..7648
                     /locus_tag="OUB02_002437"
     CDS             6965..7648
                     /locus_tag="OUB02_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886188.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            7645..9978
                     /locus_tag="OUB02_002438"
     CDS             7645..9978
                     /locus_tag="OUB02_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8886189.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEATHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(10086..10648)
                     /locus_tag="OUB02_002439"
                     /pseudo
     CDS             complement(10086..10648)
                     /locus_tag="OUB02_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10762..12111)
                     /locus_tag="OUB02_002440"
     CDS             complement(10762..12111)
                     /locus_tag="OUB02_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8886190.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSSWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(12148..13368)
                     /locus_tag="OUB02_002441"
     CDS             complement(12148..13368)
                     /locus_tag="OUB02_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886191.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDTHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         5316 a         1528 c         2041 g         4577 t
ORIGIN      
        1 aattaaaaat ataatgaaag tttagtttta attcatttat ttaataaaca tttcaagtaa
       61 aataatgttt aaatcagaaa tataaataaa tattattata atcgttatta aagaccaaaa
      121 atcccatctt agaagtttgt atcatatgaa acctttatag attatgaaag caataatata
      181 gtgtatgata tataaaataa gttataaaag tattttttta gatattcaaa aagacattgt
      241 aagttatatt aataatatgg gtatcaacga attttggata taatgagttt tttaacttaa
      301 agttatgtaa taatgaatgt aaagaatatt taatgaaatc attttgttgg ttatgtctag
      361 ctgaatacaa taatctttta gataaagatt ttatatttga agataggtgt gaaaaaatta
      421 agaagatatt aacatcagca gtttagaggc aaagcatgta aagtaaagtc tgggtaacta
      481 aaagtagtaa tttagattgt aactattgtt ataaatatga aaatgaggaa acaggaaata
      541 taagttagga tgtagaaaat aggttactaa ataaaaagct taattatata atcaaagaaa
      601 taggtatata ctgtacaatt tcatggtgaa gaacttttcc taaataaaaa ggttggtaaa
      661 agagatataa gaaataaaat tcaattataa gatatggtat gataactaaa ggcactatat
      721 ggagtgaata ggcggaagaa ttttttgaaa attataaaaa tagtttctgt tgctacatat
      781 caataagtat agatagagat tgcaaaatcc atagcaaaaa tcgaatttat aagtgtaaat
      841 aacatgtaag acaaggagta aattatgagt attttagaga taaaaaacct aaataaaata
      901 tatggtaaat cggaaacaga ggtgaaagcg ttaagtcaaa taaatctaaa aataaatagt
      961 ggagaatttg ttgtgatagt aggtaaaagt ggaagtggga aaagtacttt actacacata
     1021 atggcaggac ttgaaactcc aacaaatggt gatgtgatta ttgataatat aaatatatca
     1081 tctcttgatg aaaagaaaag atctgcatta cgtaaagaaa agttgggatt aatatttcaa
     1141 tcatataatt tgattccagt tttaactgtt gaagaaaata taaaactacc aactataaat
     1201 ataaaaaata aagatgaagc ttatattaat gaattgatgg agttactagg aataaaagat
     1261 agaagaatgt atttgccaaa tcaattatca ggtgggcaac aacaaagagt agcaatagca
     1321 agggctcttg taaataaacc atcaattgta tttgctgatg aacctactgg aaatttagat
     1381 acaaagacag agtcagatgt attaagttta ttaaaagaat ctcaaaaaaa atataatcag
     1441 acaataataa tgataacaca taatatagat ataacaaaat atgctgatag agttatagaa
     1501 atagaagatg gatttataag agagtaggta gatttatgaa acttaattat attgtaaaaa
     1561 aatatataat gggaaataag aaaaatacat tattaataat tgtaagtatt gtaatttcaa
     1621 cagcgttgtt tttaattatg aatataataa gtgaagatgc gacaaactta atgataaatc
     1681 aagctaaaaa tgaatttaca ttaaaacatg cacaatatat caatccaaaa gacgaagaaa
     1741 taaaatatat agaaaataac acaagtatag ataaagttgg aaaatcaatg cttttaggaa
     1801 ttagtgatat tggtaaaggt caaacactac aaattctatc tcaggataaa gttgctgaag
     1861 aactaaatga tatttacaca ctagaaaaag gtaaattacc agtagctgaa aatgaaatcg
     1921 ctatagattc atggtatatt aaacaaaaaa agataaaaaa ccctatagga aaaaggataa
     1981 cattagatta tagaagacaa gggatagatc aagaagattt atatactggg gaaaaagaat
     2041 ttaaaataac tggtatctta aatagtaatc ctattttaaa agctcaagga acatcaattg
     2101 gccttataag taacgattgt gctatcaaga atattcctac taaaaataaa tatgatcaaa
     2161 ttatgtttac ttttaaaaag gaaaaaaata tacagagaca atcacaaaaa cttattaaaa
     2221 atggaaattt gaatgagaat aacatttatt ttaataatga attattaata gctatatctg
     2281 atagtatgaa tttaaaaata ccttatataa tagttaatat agtactagca ctagcaacta
     2341 tattattaat ttataatatt ttttatatat tagtatcaaa tagaataaag gattttggga
     2401 tatttagggc tttagggttt aatccaaatg atatttttaa aataatgatt ttagaagtat
     2461 ctatatactc tattataagt atttctatag gtctaatttt aggtgggata atagctagtt
     2521 tatcaaggga gtatgttata ggagtaatat acaatgtcaa ctatataaat tctataaaaa
     2581 accagaacta tataaacaca tacataattt ctatattatt aagtttagga actataataa
     2641 tatcagtttc aaaacctctt atgttatcag ttaaaactga tccaatgatt tgtataagaa
     2701 gaaataatga aaaagtggat ataaaacaaa attcctttat aaataaattt atgataaaac
     2761 ttttcaaaga ctatggtaac atagcatcaa aaaatataca aagaaataag aaacgtacaa
     2821 atttatctat agcatcaatg gtaattatat ttttcttaat ggcaacaata tacacaaagt
     2881 ctactagtaa ctttttaagt gatggtgtat taaaatattg gatacaagga gattatttat
     2941 tacataatat tgatataagt actatacgtt caaataataa aggttatgat aaaaatgtac
     3001 tacaagagat aaaggcaatt gatggtgtaa caaaagtaaa ctcatatagg cataagtggt
     3061 ttaatataaa aattgatgat aaaagtataa ataagaactc tgattattgg aaaaaaaata
     3121 aagataatat tgaagctaga tcagaagtaa aagatggaat taaggtatat aataattctt
     3181 ttgaattttt aggaatagaa gatacagata tactagagga tttcttgata aatggaaagg
     3241 aaaatataga taaatttagt aaacaaccat atctgtatat aacaaagaaa tccagtgatt
     3301 cgcttaatat aaaaaaagga gatagaataa aagtatactt tgatattata gattcaaaaa
     3361 caaataattt taccaaaaca atatctcaag aatttatagt atgtggaatt ataactacat
     3421 taccagtaac ctcacaagtg ggagctgaat ttggcggagt aatatcaaca aatcaattta
     3481 ataagtttac aggaatatca tcttatgaaa gatttgacat atggacgagt aaactagcca
     3541 atgataaata tgttgaaagt gagttaaaca aaataacaga aaaaacaaat aaaggaattt
     3601 taattccata caaagctgaa acagctgaat atgaaaaaat tgataatcaa aaaacactaa
     3661 ttatggtatt agtaactgga attatagtta tattatcttt atttaactgt tgcaatacta
     3721 tagttactag tattaatagc agaagtaggg aatttgcact atttagagga ataggaatat
     3781 caaaacatga aataaataaa attgttaaat tggaaagtta tatatatatt atagtaagct
     3841 tttgtatatc tataatacct attttaattg taagaagtat aataataaaa ccttttgaaa
     3901 gtatcaattt aataaattgg aaatttatag gggctattat cctaatagta tttattttag
     3961 tatctataat catgataact acactaaaaa cattgaataa aattcaaaat gaaaatttta
     4021 tggaagaaat aaagacttta tagtgaacag cctataaatt aaagaggttt tatatgaatt
     4081 taattgcaat tatcgataat ggtgtagata ttgattttaa ataatttaga aataaaaata
     4141 aaataagtta tccaagttat atgtatgatg taataggggt acaatgtata gcaaatataa
     4201 atattggctt aataaaactc tactcttaag tctactgtcg ttttaacaca agactaataa
     4261 taagtgtaac ctaaaaatta ttgataatac aataaccttg tttatgataa ataacaaata
     4321 ttaagtgttg taaagatagt aagtttaaaa aaatatcaaa gatttcaatc acaatgtatt
     4381 gtttatctgt agtgttggtt atttatggta tatatgtatt tataaattgt actgaatata
     4441 tatatactat gctatctaca aaacagttaa cagtcacagg aaatgaatat accattataa
     4501 attattatat gagtaacttt agtcagtata taatatttgc tattttactt gcatcaatgg
     4561 gatttgtaat acaaaagata gtgcatatgc atttcagtga gatagaactt agtggtaact
     4621 attcaacaac tgttagaaat ataagtgata tctctttaga taatgataat gaaatgttta
     4681 atagaggcag tattgaaaat agtattttaa aaaaataaat aatataaata tataaaatgt
     4741 gattgaataa agcttagaat acttagtgta ttttaagctt tttttattat taagatttca
     4801 aagtattaaa ttaactatat aaaatcatat aaaaataatt ttaaaatatt taaatcattg
     4861 atttcaaggt attgtatttt taaaagagta aaaatataat aagttaggta tataatatat
     4921 ataaagtaaa aaataaaaaa atggaagtga ttgagatgag tagtatattg ttggttgaag
     4981 atgatttaag tttgatatat ggattagaat attctataca aaagaatgga tttagtgttg
     5041 atgtagcaag aacagtagaa gaagcattgc aaatttataa agaaaaaaat tatgatttac
     5101 tgcttttgga tgtatcctta cctgatggtg atggttttga tatttgtaaa agggtaagag
     5161 aagcttctaa tgtaccaatc atatttttaa ctgcttctga tgaagaagta aatgtagtga
     5221 tgggacttga tatgggtgga gatgattaca taacaaaacc ttttaaatta aatgagttaa
     5281 tttcaagaat aaaagcttta cttagacgtt ataattttac aagtgaaaat gtaactgaac
     5341 taaaatcaaa taatattact gttaaattac ttgaaaatcg tgtttttaaa aatgaaattg
     5401 agcttgagct tactacagca gaatataaat tattatgctt acttatgaaa aataaaaata
     5461 ttgtacttac aagaaaaaat atattggata agttatggga tggaaatggt agttttgtag
     5521 atgataatac tctgtcagta tatgtaagaa ggttaagaaa taagattgaa gataatcctg
     5581 aaaatccaaa aaatctatta acagttagaa gaatgggtta caaatggaat gttataaagt
     5641 gagggtgtta atatgaaggt attttctaat aaagacataa aaatattttt tttggtgatt
     5701 atatccgttt tattgatgtt tatagtagta ggtcaaatag tagttataaa tataactaat
     5761 gattataaaa gtgcactgtt agaacatgat tataatatag ctggatactt aaatagtatg
     5821 ggtgttgata aatcaaaaat accagcagta tttactacta atgaagataa atatacttta
     5881 aaaggaaaag aaatcttaaa tacagctaga tatgactttg gtacagatga tagttttatg
     5941 ccaagtgtga aattgtttca aggtaaatat ataaaaacta tgtttttgta tttatttgtg
     6001 ttctttacta tgattatttt tatattgtat gtgtattttt ttaggcaaca gaacaagata
     6061 gaagaagcta gttataaaat tgattcattt atgaatggaa atataaatgt acggttaaat
     6121 acttatgaag aaggtagttt atcaaagtta tttggttcaa tagatgctat gtcaacatct
     6181 ctaaacacac atatattgaa agaaaagcag aataaagaat ttcttaaaaa tacaatttca
     6241 gatatatcac atcaactaaa aactccactt acagcattga tgatgtataa tcaaatattg
     6301 caggaagaga gtcataatag tgaaattgtt gagaattttg tgcagaagag tcaaaatgaa
     6361 ttagagcgta tagaatcatt gatacaaaac ttgttaaaga ttacaaagat agattcaaag
     6421 acaataattc ttaacaaagc tagtgttaac gtaaaaaagt tgattactaa aattttatat
     6481 agttttgaaa ctagagcaga aaaagaagga aagagtatct ctataagagg tctagaaaat
     6541 acaaacttgt tttgcgatta tgaatggata tcagaagctt taagcaacct tataaaaaat
     6601 gcattagata atacaaaaga gaacgataaa ataatgatag aatgggttga aacacctata
     6661 acaactatta tttcagtgag tgataccgga aatggaattc atattgaaga tatacatcat
     6721 atatttaaaa gattttatag gagtaagttt tcaaaaagta atcaaggcct tggattaggg
     6781 ttgccactag ttaaatctat catagaacag cacaatggaa ccataactgt agaaagtaat
     6841 tttttacaag gaagtacatt tactctaagt tttttaaagc ttactaatat gtaagatagg
     6901 atacatctta cagtaaggtg tatgaattat tcttatatta atcatcagaa aggagataga
     6961 gcaaatggat attcttgtgg ttaataattt atgtaaaaca tatggaaatg gatatacaaa
     7021 agtagaggca ttaaaagatg tatcatttac agtttcaaag ggagagtttg ttactgtagt
     7081 tggagaatct ggctcaggta agagtacatt attaaatctt ttaggaggtc ttgatactcc
     7141 aacctcagga aaaatatttg ttgatggtaa agaaatatgt tcaatgaaag aagaaaaaca
     7201 gactattttt cgacgtcgta atataggttt tatctttcaa tcatataatc ttatacccga
     7261 attaactgtg gaacaaaata taatatttcc tttactacta gattacaaaa aaccagacac
     7321 tatttatcta gatgaagtat tgacaatttt aggtcttaaa gatagaaaaa gtcatcttcc
     7381 aaatcaatta tctggtggtc agcaacaaag ggttgcaatt ggaagagcat tgattacaaa
     7441 gccatcaatt atattggcag atgagccaac aggaaatctt gacagtaaaa atagcagtga
     7501 tgtaatttca cttttaaaag tgacagcaga aaaatataac caaactatta ttatgataac
     7561 tcataataca aatatagctt ctacagcaga taggatacta caggtttctg atggaatgct
     7621 taaagaccta ggggtgaata gagaatgaga agctatttag aactagtacc acaatatgca
     7681 aaggtacata agaaaaaaaa tagaactaca attttatgta ttgtcattgc agtatgttta
     7741 gttactgcta tttttggact agcagacatg gctatacaat gtcaaaaagt acaatttata
     7801 aaagatgatg gaaattggca catagtactt aaaaatctta atgaagaagc tatatcagaa
     7861 ataagtagta gagtagatat agctcactat ggtagaggac aagtcattga taatggagag
     7921 tttaaatcaa aggagatttt gatacaaggt ttagatgaag atttagcaaa acaattaggt
     7981 ctagaaataa taaaaggtcg ttatcctgtt gtggagaatg agactttaat agatgtagat
     8041 gcagttaaaa gctttaatct aaaagtagga gattttatca aaataaaatt agcagatggt
     8101 agaaataaaa attataagat agtaggtgaa tattcatcat tatcaagctt aagagcctca
     8161 gataagcatg gattaatgct ttcaatatta ggtacacaag ctatcaaaaa atcagaaaca
     8221 tatgattatt attatattac atttaaagac aaggttaata ttagaaaagc tacttctgat
     8281 attaagaata cctttaattt aagtgataaa caaatcggta aaaatgaaaa attactttct
     8341 attataggtc aaggtgactc tgattattct aaaccacttt acttaacagc aggaggactt
     8401 tttatacttg tgttaatagc aagtattatc atgatatata atagctttaa tatgggtgta
     8461 atagagagaa taaagttttt tggactatta cgttgcttgg gagcatcaaa actccaagtc
     8521 aaaaaatttg ttgttttaga aagtttaatt ctaagtgtaa aggctattcc actaggattg
     8581 ttattaggat gtattgtaac cattatatcc tctatatttc tcaaatatgt gaatacagag
     8641 ttatttggtt cgatgccaat tttaaaggtt agctttattg gtattgtgtt tggaataata
     8701 gtaggatttt taacagtaat attatcagct attgttccag caaaaaaagc ttctggggtg
     8761 tctccactaa gtgctatacg tggtaatcta atgttcaaag aaattttaac tagtggaaaa
     8821 gcaagtaaaa ttgctaacgc aggaagttct aaaattgatg tttctatggg aataaatcat
     8881 gccttttcaa gaaaaaaatc ttttatactt atgacttctt catttgctat tagtattgta
     8941 ttgtttttag gttttagtgt atttatagac tttatgtatc aggcacttaa acctataaaa
     9001 ccagaagcta ctcatatatc tattgttgac gctgacttta agtctgttct tactaaagac
     9061 gatattaatg aaataagtaa tatcaaagga ataaaaaaag tatacagtag aatgatgacg
     9121 gatgtagagg ctacatacaa taataaggag gcaacatctc agctaatttc ttatgaaaaa
     9181 aatcaatttg actgggccaa aaagtatcta gtaaaagggg aaatcaatga agataaatta
     9241 gagtcagaaa atatcggatt ggtagaagaa ggtcatgggt ttaatattgg agataagata
     9301 tatattaaaa atggaaattc taagaaagag atagaggtag caggtatact ttcttcaatt
     9361 tcatttgatg ttaaagacaa atatattgga aatattataa catctgaaaa tacttttact
     9421 agtataacag atataaaaga ctatataata attgatactc aagttgatga agatgctcct
     9481 gaagatttaa tccaaaatat aagaaatgtt ctagatgata atttagaggt tcgagataag
     9541 cgacaaggta acttagaggc taaaaattca ttttacacta tggcagtttt tgtatatggg
     9601 tttgtattta taattgcgat tataagtatg tttaatatca taaacagtat gaatataagc
     9661 gttacaagta gaataaatta ctatggtatt atgcgtgcaa taggcatgtc caataagcaa
     9721 ttaagaaaaa tggtaatagt agaatcaagt acatatgctg taagtggctg tttattgggg
     9781 agtgtacttg gattaatatt acatagatat atatttgtat ccttagtaac attaaaattc
     9841 catatagact ggcaaatacc ttttgattta ctgtttatca ttgtagtaac tatgataata
     9901 ataactttgc ttgcagtaag aaaacccatt aaaaagattt gtgaattaga cataatagag
     9961 gtagtaaatg cacaataaat ataagtagta atttaaatag aggtagtaat ttaaataaaa
    10021 aatcgaagta aatatgaaat agaaaatatc tttcataatt tacttcgatt tttgtgttat
    10081 ataatttact ttagataagt tccatcttca tcttgataaa ttaagtcttt cttcataagt
    10141 actccaagag cacgtttaaa attttcttac ttgaattaaa taagatagaa atatctttag
    10201 gatcagattt atcattgaat ctcatatatc catctgaacc ttctaaatag cttaaaatct
    10261 tattttctaa ggtatcaagt tcatcttttc tgtttcctct aggagaaagt cctaatttac
    10321 catcatcgta tatttttata acatgtaaat tttcaagaac atccccagct tttaattctg
    10381 taaagtattc attatgaagg attacaccag catatttatt gtcaacacat atcatagcag
    10441 agttgttggt ttggaatcca tatacaactc catttacaac atctcctaca ttgtaagtat
    10501 ggtctgtttc taaatatgaa tctatgtctg tagtagcagc tattcttccg cttttatcca
    10561 agtatatata gaataaatat ttttcatctc taaacaatgg ataagtctta gctttaaaag
    10621 gaactagtat gtcttttgta agtcccatat ctataaaagt accaatatcc gtgttatcaa
    10681 ctacttttag atacgtaaca tctccatttc taattatttc tatagtatta gaaaaataat
    10741 ttttaaatag tttgtaaaat attattgaat tttttctctc gaaatatata atttagaatc
    10801 ttctgattta tttttatgct cgatttcctt attttttttc ataaatatat atagaggagc
    10861 tattaacaac attgccccta atcctattaa aactctattt tgagttatag gttttgaaat
    10921 atcttctgcc caagcttgtt gtaacaacca tgagctacct aaaatagcta ccattggtat
    10981 aacaggacca aatggaatct taaaagaggc cttaacatct gttctttttc ttaaaactat
    11041 tactgataaa caagttggta tgtactgagc aaatcttgca attacactca ttactgcaag
    11101 ttgttcaaaa cttccagtta gcacaagtgg tatacaacaa attaaagata ttattattgc
    11161 tacatatgga gcaccatatt tatttgtttt tcctataaat gcaggaagag aaccttcttc
    11221 tgctagagaa gaaccacatt taggagttac tattgatgaa cctatattta taccaaatat
    11281 agatattaat gttgctacag atatgaaaac tttaccataa ttgcctaaaa atacacttga
    11341 agcatctgca attggaatac tgttttcaaa tagtctatct ccaagtatac ccatacagac
    11401 aatttgaatt aatatataaa ttattgcaca tataaatatt gtggttatta atgcgactgg
    11461 aagatttttt ttaggatttt ccatttctcc acttgccaca ataaatgatt caaagccggt
    11521 aaatgcgtaa aatactaata taatggctgg acccatacta gagttaacta catttcctga
    11581 aggaacaata tttacaaatt ttataaagaa tattccaact attataaaaa ctattaatgg
    11641 tactaattta gctatagtta ttacattatt cattatcttt gtgcttttaa ttccaaataa
    11701 gctatttatt gaaagtagtg ttacgagtat tgcagctata taacctttgt attcagtggc
    11761 agattctggc caaaatgaac ctaaagctgt tgcaaatcct actgctagag tagaccatga
    11821 tataattcta ataacccaag acattgtacc aacttcaaat cctataaaat ctccaaaagc
    11881 ttttttggag tatagatatg ctcctccatt cttatcaaac atgcttccaa cttctgcaaa
    11941 acataaaagt attgatagaa ctaagagtgt agcaaaaatg tatataaaca tactgttttg
    12001 acctgctaga ttataaactt tacctggtag taagaatata cctgaaccta ttatgctatt
    12061 aatccctaat aatatcatac taaatagacc taatttctta gctttttcca tattaattta
    12121 tctcctattc ttttattaat tactgtacta attaaaatac aaagtattca ttacatgcat
    12181 cataacacct attggtaaac aatcttcatc tatattaaag ctagaggtat gaagaggtga
    12241 aattaagccc ttttcctcat ttttacatcc taaatggaag aatgctcctt tacaatgttc
    12301 tgtgtaaaaa gaaaagtctt caccacctaa agacgggttt ggtctcaata taaatttttc
    12361 ctctccaagt aattctttag tatttgatat aactgtatct actaattctt tttcatttat
    12421 aacagcagga tagttttcat cacttacatg taaagtccca acacaaccaa atgcagatgc
    12481 agtgtctcca acaattttag ctattttatc aatcataaag tttcttgttt tgctatttaa
    12541 ggttcttaaa gtgccttcta attttacatc ttcacatatt acattgtgtg tatcaccacc
    12601 atatatttta cctatagtca aaacagcagc attatttggt tctaagtttc tacttattat
    12661 agtttgtaat gatgtaacaa tttgtgaagc tgtaactatt gcatcaatac cgttttcagg
    12721 atatgcacca tgagctcttt tacctttgat acttattttt atagtgtcta cactagcatt
    12781 taatgtatca tattttgttt cgataaggcc tgtttctata tgtggcataa catgaagtcc
    12841 gaatatataa tcggccttgg gattttctaa acacccatcc tcaactaaaa attttgctcc
    12901 tccaaaacct tcttctgctg gttgaaataa gaattttaca tttacattaa gtttatcttt
    12961 tattgaatgt aaaactttac atgctccaag tagcatggtt gtatgtgcat cgtgaccaca
    13021 agcatgcatt tttccagagt gtattgattt ataaggtatg ttgttttctt cttcaatagg
    13081 aagagcatcc atatctgctc ttatacaaat cgttttatct gcactttctt ttaaaatata
    13141 tgccataatt ccattgtgtt ttgtaaattc catataatca attccgattt cttttaaata
    13201 agaaattacc ttctgtttag taagattttc ttcaagagct aattctggaa ttttatgtaa
    13261 ttctcttctt atgtttataa gccatggttt tatagaatta caactttcta ttacaaaatc
    13321 ttttcctaat tctttggttt gatattttaa tttcaaagcc tgttgcattt ttattcctcc
    13381 aattgctttt tattgtttta ttaatataat aagcaattat tatgccataa ttttcgatgg
    13441 agaattttgt tttttataag aa
//
LOCUS       DAKBNN010000105        13304 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000105 DAKBNN010000000
VERSION     DAKBNN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.105"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            89..1165
                     /locus_tag="OUB02_002442"
     CDS             89..1165
                     /locus_tag="OUB02_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT8886192.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1282..2124)
                     /locus_tag="OUB02_002443"
     CDS             complement(1282..2124)
                     /locus_tag="OUB02_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT8886193.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2308..2880
                     /locus_tag="OUB02_002444"
     CDS             2308..2880
                     /locus_tag="OUB02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8886194.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSKNEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3043..3315)
                     /locus_tag="OUB02_002445"
     CDS             complement(3043..3315)
                     /locus_tag="OUB02_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886195.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3442..4314)
                     /locus_tag="OUB02_002446"
     CDS             complement(3442..4314)
                     /locus_tag="OUB02_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT8886196.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4610..7717)
                     /locus_tag="OUB02_002447"
     CDS             complement(4610..7717)
                     /locus_tag="OUB02_002447"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCT8886197.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7810..8042)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8217..8744)
                     /locus_tag="OUB02_002448"
     CDS             complement(8217..8744)
                     /locus_tag="OUB02_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCT8886198.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8779..9564)
                     /locus_tag="OUB02_002449"
     CDS             complement(8779..9564)
                     /locus_tag="OUB02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCT8886199.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYERAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9574..9831)
                     /locus_tag="OUB02_002450"
     CDS             complement(9574..9831)
                     /locus_tag="OUB02_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCT8886200.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9850..10308)
                     /locus_tag="OUB02_002451"
     CDS             complement(9850..10308)
                     /locus_tag="OUB02_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCT8886201.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     TKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10348..11052)
                     /locus_tag="OUB02_002452"
     CDS             complement(10348..11052)
                     /locus_tag="OUB02_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCT8886202.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11062..11697)
                     /locus_tag="OUB02_002453"
     CDS             complement(11062..11697)
                     /locus_tag="OUB02_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886203.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12078..13118
                     /locus_tag="OUB02_002454"
     CDS             12078..13118
                     /locus_tag="OUB02_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8886204.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4225 a         2258 c         1575 g         5246 t
ORIGIN      
        1 tttatatttc tagaagaaaa gtatactctt ttttgtttaa taatgctaaa attgatttgt
       61 attgaatttt gattaggagg aaattattat gaaagctcaa agattaaatg ctgtacttga
      121 acaaatgaaa aaagatgata tttctcaaat gttagtaagt gaccctactt ctatatttta
      181 tcttacagga gtactaatcc atccaggcga aagactatta gcattatact taaatttaaa
      241 tggcaataat aaattattta taaatgaatt attcccagtt tcagaagatt taggtgttga
      301 aatggtttgg tttaatgata ctcaaaatcc agtagaaata ataactgaac atatagacaa
      361 aaatgctact atgggtgttg ataaaaactg gccagctaga ttcttattaa acttaataga
      421 attaggtgga ggaagtaagt ttgtaaattc atcttatata atagatactt taagaatgtg
      481 taaagatgaa gaagaaaaag agttaatgag aatagcttca aaattaaatg acaaggctat
      541 ggaacaactt aaagctactg tttctggtga acttacagaa aaacaacttg ttggtaaatt
      601 atctaaaata tatgaagacc taggtactga tggattttca tttgacccta ttataggttt
      661 tggacctaat ggagctaatc cacatggtga acctggaaat gcacttgtga aaccaggaga
      721 tgcaataatc ttagatatag gttgtataaa agataactat tgtgctgata tgacaagaac
      781 tgtattctat aaagaaatac ctgaaaaagg tagagaaata tttgaaatag ttcttgaagc
      841 taataagaga gctgaagcta ttgtaaaacc aggtgttaga ttctgtgata tagatgctgc
      901 tgctcgtgac tacataactg aaaagggata tggacagtac ttcactcata gaactggtca
      961 ctctatagga cttgaagttc atgataaagg agatgtatct tctataaata cagatactgt
     1021 acaaccagga atgatattct ctatagagcc aggtatatat ttaccaggtg agttcggagt
     1081 tagaatagaa gatttagtac ttgttactga agatggttgt gagatattaa acaaacatga
     1141 caaagaaata tgtgtagttg gttaattcta aaatcaattt gataaaatta aaaagccata
     1201 taaaaatgtt ttaaacattt ttatatggct ttaatttctc aatctatgat ttatttcaat
     1261 ccattattta tattgcagta tttatattgc agttattaag attgcaccct ttgaagtata
     1321 tgtccccatc actgaaccaa tttcacagat tacaacatct ttgtatttta catccttttc
     1381 cataacctcc ctcacctttt gagctttttc tgggcaatta gcatgtccta taaataaaac
     1441 cttttcttct ttatctttaa tttttgactc tactaaagta atcagctttt tcaaactgtt
     1501 tccttcacct cttgccttat ctataggagt tacaacgcca tctgcaactt gtattatcgc
     1561 cttaaaattc aaggtattta taatttttcc tgctattgga tttattctac cacccttaat
     1621 agcattttct aaagtttcaa gtgttccaaa aaatggagga ttatctttat atttatttat
     1681 tattgaaaca atctcttcaa tttcttttcc ttcttttatt aatattgctg cttttaacac
     1741 taattgtcct attccaattg acccactaag tgaatctata acctcaactt tcttgttatt
     1801 tccttccttt tcaaacatat cttttgctaa aagtgcacta ttataagtcc cagataatcc
     1861 tgaacttata ttaatcacta atacactttc ttcctgacaa tggtatgctt ctaaaaattt
     1921 atcaggagaa ggacatgatg tttttggtag tactttactc tcactcattt ttctatagaa
     1981 agtttcatta tctatttcct ttccagatat ataactttct tctccaaaag atacatttaa
     2041 accaacaact tctatgttgt atttttctat tatatcatca ggtaagtcac atgcactgtc
     2101 cactatcaat tttattttat ccatattaac ctcactataa gatattattt tatttattca
     2161 gtcggtcgta cgaccgattt aattattatt ttatatattg aatgaaaaaa agtcaataga
     2221 tatagattct cgctttattg acaataaata tccataagct tataatgtta ttaatttgtt
     2281 ataaatatac atggaaaggt aggttttatg aaaacaaata atatgaaaca ggctatttta
     2341 tcatctgcaa ccaatttaat agccaaaaat ggagttcaaa atactagcct tgctgatatt
     2401 gcaaaagatg taaatataag taaaggtact ttgtattatc actacgcatc taaagatgat
     2461 attatatatg atattgcgga tactcattta gaagttataa ctagtgctat actaaactgt
     2521 gtaaaaaatg tcaaatcaaa aaattctcaa atagaaatgg ttaacttaat cttagagaaa
     2581 atatctacta tagaaagtcg tggtagggta catatgtatc ttatttgtga ggcaattact
     2641 tcaaataatg atttaaagga aagaataaga ttaaaatata ttgaatggag aactactctt
     2701 aaatctgaaa ttgttgaatc actagaaaaa tataatagta gcaaaaatga acaggatgct
     2761 gaatcatttt cttttttgct catgtcaata gttgatggtt tagttgtcca aagtttatta
     2821 aaaacagaaa aaatacctta tgaaaatata gcttcttttt tagttaatca ttggatttaa
     2881 acttaatact tagataatta ataaaagtga actatttaag ttatctactt taacagttca
     2941 ctttcttatt tattctcaat aatttttact taaaacattc aatttttatt taaaccttca
     3001 tggaatcaca aaataaaaat ctcttataaa tttaaaatgt ttttatttat gttcatgtgt
     3061 atgcccatct gaatgttcat gaccatgtgt atgtgtatgt tcgtggctat gctcatgagt
     3121 acctgaaaaa ccatcatcat gattatggtc gtgacctaca ccatttacat gctcatgttc
     3181 atgcccatga ctgtgagaat gttgatgtga atgttcatgg ttatctcctc cacagcatcc
     3241 ttctcctcca gtcccacaac acccacttgt atgtggatta tgatgatttc ttactacaaa
     3301 tctttctctt aacataattc ttatacctcc tagtatatta ttgagtactt catgtacccc
     3361 attaactaca gtatacatct aactaggtat aatctcaata catatggcat tttttaacat
     3421 atataattaa catatataat cttaaagaaa tttatcctta aaacctccac cttttacttc
     3481 ttcaacgtta ttgacagaaa taaaagcttc actatccata tcataaatta aatctttaaa
     3541 tttacctatt tcatttgcag atattatgca ataaatcatc tttttcttat tttgtgtata
     3601 tgcaccttct gcctccaaaa aagtgactcc tctacccatt ttattcatta tcactttgga
     3661 tatttcatca tatttatttg atattaaaag tatagatttt tttctatcaa agcaatcttt
     3721 tcctatatcc atagtaaaag aaattatata cattgtaatt aaagtgtaca tcaccaattt
     3781 agctccaaat aaaaaagctc ctgcacatac tacaaaaaaa ttaataaata aaaatgtatt
     3841 tttcataggt atatcatact ttcttttcat tatggctgct attatatcaa gtcctccagc
     3901 agtagctctt gctttaaaag ttaaccccat acctatacca gttaatgcac caccataaat
     3961 acactgtagc atagtatcat ctattgggaa atatttagca atatcttgtg ttagccccag
     4021 tgctagagaa aatagtaaca tattgacaaa acttattagc aaaaatttat tatcaaaata
     4081 ctttctagct ataataaata ttggtatgtt catcaagaaa cttgatattc cctgatttat
     4141 accaaataat ttgtataata taacacaaat tccagctact cctccactta ataacttggc
     4201 tggtgctaga tataaattta ttcctaaggt tgatagaatc atacccaata taataagaca
     4261 atattgtaaa aatatgttat tatcaatact tagcttttta ctttcaccat acatcaaaac
     4321 gcaccccttt gcataataaa taaaccaccc tgcagaacac acgataaatt tacaaataaa
     4381 tttatcagaa gtagcttact aaaaatatta ttcatcctca cttaaacgtt tattcattaa
     4441 tattctatca catgacataa ataatttata tgatttatat agatttattt attgtaaatt
     4501 ttggaatatt aaaatatttt attgaataga taattatatt acataaaaaa tccctataga
     4561 aatctataaa ctaatttcta tagggatttt ggttaactaa ctattctctt tattttttta
     4621 taacttttat acctgtcata tggtcattta agttttgttc ctctaaagat ttatcatcta
     4681 ctctgtttat ttctaaagct aaagtttcag acttgatata ttcattaaaa tggtcaacag
     4741 ccttagcaat ttcatcatca ccacagtatt ctattactat attatctaat acttcaaagt
     4801 tattagattt tcttaattgc tgtactttag atataaattc tctagcataa ccttcattaa
     4861 tcaactcttc agttagctta gtatctagta taacaaaaag attgttttca acagatacat
     4921 taaatccttc cttagcagtt atacctatta atactaaatc cttattaaat tcaaaagctt
     4981 caccatctaa atcaactgtt aaagtctcac ctttttcaag tttattagca gtttcatgag
     5041 catttaattt acttaatgct ccaacgaata aattcatctt agaacctaat aatggccctg
     5101 cttccttaaa gtttggtttt aaactaaagt tcatatactc atctaaatcc ttagcaaata
     5161 ttacctcttt aacattaagc tcttctttta ttaaatcaac tacatcactt attgtatctt
     5221 caaacttacc atctactaac actttttgta atggttgacg aacttttatt ctttctactt
     5281 ctcttgaagc tctacctaaa gtaactaagt ttttagctaa atccattttt tcttctaatt
     5341 tagtatctac taactcttca ttacatttag gatattcagc taaatgaaca gatacttctc
     5401 ctgttaagtt tctatacatt tcttctgaca tatatggtgc aaatggagct atagctctac
     5461 aaagaccatg taacaattca taagttgtat tgtacactgc ttttttatct tctgttaatt
     5521 cagttgccca gaatcttctt cttgaacgtc ttatatacca gttagataaa tcatcattta
     5581 taaaatcttg tatcattctt aaagttttat ttaactcaaa tatagctaaa ttttcttcta
     5641 catctttcat taaattattg aacttagata atatccatct atcaagttct ggtctatctt
     5701 tatattctac aaaaaattca gttggattta tatcatctgt gtttgcatat aaagtaaaga
     5761 agttatatac attcttcata gtacctataa atttactttg gatttctttt aatccatcca
     5821 tatcaaattt agttggagtc catggtggag aaacatatag taaataccat cttaatgcat
     5881 ctgctccata ttggtcaaat aattccattg gatttactgt atttcctctt gatttactca
     5941 ttttcttacc atctttatca agtacaaggt ctggaactaa aacactctta tatggtgctt
     6001 ttcctaatac aaatgttgat actgcaagta atgagtagaa ccaacctcta gtctggtcta
     6061 taccttctga tatatagtca gctgggaaca attggtcaaa gttttcctta ttttcaaatg
     6121 gatagtggtg ttgagcaaat ggcatagccc cactatcaaa ccaacagtct ataacctctg
     6181 ttactcttgt cattggtttt ccacattttt cacattttaa gtgtatatca tctacatatg
     6241 gtctatgaag ttcaactgtt tctggattta catcttctat agctttttca gctaattcag
     6301 ctcttgaacc aactgaatct gtatgaccac attcacatct ccatatagga agtggagttc
     6361 cccaatatct agttcttgat attgcccagt catttagatt ttctaaccag tttccaaatc
     6421 tcttctctcc tacaaaactt gggaaccatt ttacaccatt attattttct attaatttat
     6481 ctttaagttt agtcatctct atataccagc ttggttttgc atagtatact aatggagttt
     6541 gacatctcca gcagtgtgga taattatgaa ctactttttc tttgctatat aatttatttt
     6601 cagaagctag ccattttatt atttcaacat ctagcccatc ttccattaca aatctacctt
     6661 cccaaggagt agatgtaaat ttaccagttt catctactgg ttgaataact ggtaaatcat
     6721 atcttttacc tgtgttgtag tcatcttcac caaatgctgg tgctgtatga actataccag
     6781 ttccatcttc agtagttaca taatctccta ctgtaataaa aaatgctttt ttgtcaactt
     6841 ctacaaaagg cattaattgt tcatattcta aaccttctag gtctttacct ttcatttctt
     6901 ctaaaacttc ataatcttca cctagtactt tatcagctaa aggtttagat acataatata
     6961 cctcatcacc ttttttaact tttaaataat ctacatcagc atttactgtt aatgaaacgt
     7021 ttgatggtaa agtccatgga gttgttgtcc atgctaagaa gtattcatca gcatcttttt
     7081 tcttaaattt agctataact gtattagttt ttacttcttt atatccttgt gcaacctcat
     7141 gactagcaag accagttcca catcttgggc aataaggaag tattttatgt ccttcatata
     7201 tatatccttc tttgttgaat ttgtttagta tccaccatac agactctata tagttattat
     7261 ctaaagttat atatggatta tctaaatcaa cttcataagc cattctctca gtcattattc
     7321 tccattgttt ttcaaatgag aaaactgact ctctacattt ttggttaaat tcagctattc
     7381 catattcttc tatttgttgc ttgctagtaa gacctaactc tttctctact tgtatttcaa
     7441 ctggtaatcc atgtgtatcc caaccagctt ttctctttac ttgataacca gacattgttt
     7501 tatatctaca aactgaatct ttaagcgttc tagataaaac atggtgtact ccaggatttc
     7561 cattagctgt tggaggtcct tcatagaata cgaaacttgg gtcatctttc cctttttcta
     7621 aagttttttc aagtatattt atgtctttcc aataatcaaa aacttgtgct tctgcttgtt
     7681 ttacagaact gtctaccaat ggcttaaatt tcgccatgta aatcaccctt tcctactaaa
     7741 tattataatt ccaaaatttt ttattctttc acataaattt aaatactaaa aaactcgccc
     7801 cctttaaacc aaagggacga gttataaatc gcgttaccac cctaattcta tatgtaaact
     7861 aaatactagc tacatataac actctaatag tataacggta ctaaccggca cagcttagta
     7921 gaatttaatc gttcagcctg cagctcaaga gtgattttta tctttataaa ttcattggtt
     7981 ctcagcatac ccaattctct gtaaaattat ctaaagataa atgtctctat caacgctttt
     8041 aaaaatttat ctgtgtttat atgaaattct ataacaaata atttgaatag tcaatatatt
     8101 tttgaaaaat ataataattt ctatttttct tacattttta tcctattttt aatctttgtt
     8161 atagatttct caaactcaca caattattct acataaaaaa taaacaaatt acaagcttat
     8221 tctaaagttg tagcagcttc atcattacta taattataca ctgttgttcc ttcaaatgcg
     8281 tttacacatt tttcatctac atcataaaaa atctcatcaa cacttcttat ttcatcttct
     8341 aacagtgatt taaatttatt tctaaatatc ttaaattctt tgactaaagc atcatactct
     8401 tttcttatct ctataacacg gttattagct tgttctataa tttgtctagc ttttaaatca
     8461 gcttcttcaa caattatctt agccttttta ttagccgcac tacaagtgtc ttctgctgca
     8521 ctttgagcag ttattaaagt ggcttttagt gtttcttcaa tattttcata cttgtttact
     8581 tggtcttgat ataaccctaa tttttcttta agagctgtat tttccctaca tagagattca
     8641 taatcttcct tgatgatatc taaaaattca tcaacctctt catctctata ccctcttaaa
     8701 ccttttttaa actctttatt ttctatctca attggagtta gcattacatt tcctcccagc
     8761 tataatttaa attagtcctt atacaattaa tcttgcttga acttttattt ttcctttttt
     8821 agtaatatct cctatttgtg ttaccttagc tcttcctttg cctctaatag aaatcaaatc
     8881 atcatttttt actattttag aagttgaagt tatcttttca taattgacat ggactttttc
     8941 tccatttatg tattttgcac tatcttgtct agatatattg taaataccac ttatcacaca
     9001 atctaaccta tctgaagata ctgtaaatga tacttcttta tattttgaag aactattaac
     9061 aatattttct ctggaaattt cctttacaat tacattattt cttgaaacct tttctaagtt
     9121 tacaattata aaatcgcaaa tatcactact tacaactact tgacaaaagc tatcatgtat
     9181 aattatatct ccaatttttt ctctttttat acctagaccc agtattgacc ctaagtaatc
     9241 tttatgagat atatttttaa atttgaaatt cccttctatt tgtaaaaatc taagagtatc
     9301 ttctatatct tcatattcca tgtaaaatgg ataaataaat acagtgcttc tttcagctcg
     9361 ttcataacct ccatccacac tgtattttat ttcatttgta gaatttaata ttgctacagc
     9421 atttttcact tcatatggat taagaaactc cgtagactta acatcatagt tttttataca
     9481 actattagcc ttgtctatca ctttaaacat tttatttttc aagtctatat cctttatgtg
     9541 atttgttagt tttaatttat ccatcctatc accttagaat ataaccaata ctaattgtct
     9601 taggaacatc aataatacta ttgcaattaa aggagaaaaa tctataggtc catttgtata
     9661 tttatacatt atagacctta ttggcccttc tattggctct gtaagatttt ccaaaatatc
     9721 aaaaatttta ctttgtcttc catttggaaa ccaagtcatc aagcttttta taactatcat
     9781 ccatgctatt atatctaaaa gataataaag tgcaattctt attgttccca tatattaacc
     9841 gcctttttac tatttatttt gccaagggaa gaatgcttta ctttctaatt cttttttcat
     9901 atttgcatct atatccacat tattaggagc tagtataaat attgatttag aaaccttttg
     9961 tatacttcca tctaagacat aaactgcccc attcataaag ttaaatatag atttacgaac
    10021 ttcagaatta gtcaatcctt ccaaattcac tataactgcc cttctttgtt ttagatggtc
    10081 tgctatagtt gcagcttcat cataaacctt aggttcaact attacaactt tcatttggct
    10141 agttgtatgt aaatttacaa ttttatttgc ttttgtagta ctaaagccac tgttcatttc
    10201 ttcttcttga actatatcgt ccatgccact ttcatattca tcttcaatgt aatcttcttc
    10261 ttcatcaact atccaattct taaatttaga tattattcca tttgacatta ttaacttcct
    10321 ccttaaaatt tattcaaatt tgagtaatta ttttttattg tagtttcttt ctccaaagat
    10381 tgatgttcca actctaacaa tggtagcccc ttcctctata gcaatttcat aatcgtgact
    10441 cattcccatt gacaaaacat ccatccctac attagggatg tttagttctt ttatttgttt
    10501 tgataaatct ttaagacctt taaatacacc tcttatctca tcttcatctt ctataaaagg
    10561 tgccattgtc attagaccct ttatttttat atttttatat ttttctgaca cttctttgac
    10621 aaagtctaca acttcttctc tatcaagtcc atgcttagac tcttctttag atatattcac
    10681 ttgtactaag caatctattt ctctgtttat ttgttctgca cgtttttgaa tttcttcgca
    10741 taatgcaatt ctatctaaag agtgtatcat acaaacttta tcgattatgt acttaacttt
    10801 attggtttgc aaagttccta tcaggtgcca gtttaattta tcacctataa tatcatactt
    10861 tctagctaat tcttggggtt tattttcacc aacagaagtg attccttccc tcattgcctc
    10921 attgactaag tctacatcaa cagtctttgt gactgccaaa agggtaatct ctttgctgtc
    10981 tctatctact ttttttgcag ctttatcaat tttgtttctt atctcagaca aattatcttt
    11041 aatagaaccc attattatca cctactttat ccttatattt gtgtttatac tgttaggtct
    11101 taaaattatt ttgtcatata gattaacact tgtaactcca tttatgtcat ttttataata
    11161 atccacaaat acagaagatt cattctcata agttattcct tctaatatga caaattttac
    11221 actttgtgat tgttcatcaa ttacatatac accacgttta ttgtcttttg tttttataga
    11281 agttttaggt atttttaaac attccatctg attatatatt atatcaaatt cttcgacact
    11341 tgtatcctga tttgcaacaa tttggttact tatttttaat tttaccatta ttttattatt
    11401 attttcatat attttgtaaa cttttgcatc taatttttgc tcatgatatt caattattac
    11461 attttgattt ttttcaaagt tattagaatc ttctttactg atgtatgtag atagataatt
    11521 actctggtca ttggcatctt tttctatact tccttttaca tgaatctttg catcaacttt
    11581 ttcatttttt aacaccaacg tatttacatt tttgctaatt atcattacta atatattaaa
    11641 tactaaatat ataagaatac caagtatcac taatctggta tattttacct ttttcaaaga
    11701 ctctccccct ataaaattac tttcattata agtacttctt ctaaaaaatg tttattcctt
    11761 ttttataagc taatatttta cgttctaata attatttttt ccatattaaa tttatttttt
    11821 atcaatcttc ctatacaaga ctgcattatt tatatataat tgtattataa agtataagtt
    11881 taatttacat tttatacgtt aagcttactt tatatgtttt tatagttatt tttattttta
    11941 gatacaatat aaaaccaatt aagatttaaa ataacttttg ttatgttgac atttatttag
    12001 gtaatcattt acgaatcagg ataacgttat cataacaaaa gtttatgtcc taaaaaaaat
    12061 acaaggagga ttttcttatg tcagaaattt tattaggaac aggtttactt ttattggttc
    12121 tatgttgttt cacaattttt agttacaagg ccccatatgg tatgaaggct atgggagctc
    12181 tagcaaacgc tgcatgtgct actttcttag tagaggcatt caacctttct ttacttggtg
    12241 atgttttagg tatggaattt tttagaagtg ttggagctgc taatggtagt cttggtggag
    12301 ttgctgctgc tatacttgtg cctttagcct taggtgtatc tccagtttat gcagttttaa
    12361 taggattatc ttgtttagat ttttctatat tatctggatt cgttgctgga tactttgttt
    12421 ctttcttaat taagtttata gagaaaaaag tgccacctgg agtggattta gtggttgtaa
    12481 ttcttctatg tgcaccacta tctcgtctta tagcatcaaa tgtagaccca ttagttaata
    12541 gtacactact acaaattgga caagttttga tagaagctac caacattagc cctataataa
    12601 tgggaattat actaggaggt ctaataacag tagttggaac gtctccactt agttcaatgg
    12661 ctcttacatc tataattggt cttactggtc ttcctatggc aataggagca ttaggtgctt
    12721 ttggttcagc ttttatgaac tcaacactat ttgcaaaatt gaaattaggt tctaaaaaag
    12781 atattatatc tgttgctatt gaaccattaa cccaatcaga cataatttca gctaatccaa
    12841 tacctatata ttctactaat ttctttggag gagccttttc tggtatcatt gtagccttga
    12901 tgggtcttgt aaacatgaca ccaggtacag ctacacctgt ggctggtttt gctataatgt
    12961 ttgcttataa tccagttcaa caagttctta taacagctgg tttatgtgct ttattcaatg
    13021 taattgcagg atttttaggt tcaaaaatat tcagtacctt taaagttctt actgctggtg
    13081 aaatacgtgg tactgatgta gaagaagaaa ttgcttaaaa aacaataaga tttataaatt
    13141 tatatctcaa gtagtagagt tttatattgt aggcaatttt attaattgca aacgttttat
    13201 tttttattta tgtttatgat tattatgtgt gttgaatatt tagataaatt tttatttatt
    13261 atttttattt agatttttgt ttagtttttt atttggttga tata
//
LOCUS       DAKBNN010000106        13173 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000106 DAKBNN010000000
VERSION     DAKBNN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.107"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            196..393
                     /locus_tag="OUB02_002455"
     CDS             196..393
                     /locus_tag="OUB02_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8886205.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            422..1498
                     /gene="vorB"
                     /locus_tag="OUB02_002456"
     CDS             422..1498
                     /gene="vorB"
                     /locus_tag="OUB02_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HCT8886206.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            1501..2220
                     /locus_tag="OUB02_002457"
     CDS             1501..2220
                     /locus_tag="OUB02_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HCT8886207.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            2233..2772
                     /locus_tag="OUB02_002458"
     CDS             2233..2772
                     /locus_tag="OUB02_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HCT8886208.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            2929..3987
                     /gene="buk"
                     /locus_tag="OUB02_002459"
     CDS             2929..3987
                     /gene="buk"
                     /locus_tag="OUB02_002459"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HCT8886209.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4005..4898
                     /locus_tag="OUB02_002460"
     CDS             4005..4898
                     /locus_tag="OUB02_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HCT8886210.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNVLL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            4928..6136
                     /locus_tag="OUB02_002461"
     CDS             4928..6136
                     /locus_tag="OUB02_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT8886211.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            6160..7419
                     /locus_tag="OUB02_002462"
     CDS             6160..7419
                     /locus_tag="OUB02_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8886212.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGTLAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            8064..9527
                     /locus_tag="OUB02_002463"
     CDS             8064..9527
                     /locus_tag="OUB02_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCT8886213.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKLFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            9583..10719
                     /locus_tag="OUB02_002464"
     CDS             9583..10719
                     /locus_tag="OUB02_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8886214.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            10955..12070
                     /locus_tag="OUB02_002465"
     CDS             10955..12070
                     /locus_tag="OUB02_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCT8886215.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLFCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            12222..13091
                     /locus_tag="OUB02_002466"
     CDS             12222..13091
                     /locus_tag="OUB02_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8886216.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGNLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         5089 a         1425 c         2559 g         4100 t
ORIGIN      
        1 aaattaaata ttcttaaaat ttaaaatgtt attgaaatat ataagcgaat gtgttatctt
       61 ttaattaagc aataaaaata gaacaaggtt ctatactata gaacaaaatt gatgcatata
      121 tagtatttat acaaaatata aatattatat atttaaaatt aagtaaaaat attacataaa
      181 ggggagattt gagaaatgga aaaagtcaag ttgagtgttg aaagatgtaa gggatgctat
      241 ctttgtattg aagcttgtaa aaaagatgca ataagtcttt ctgactacac aaataaaaaa
      301 ggctttgtaa ctattaaagt cgatgaagaa aaatgtattc aatgtggcgc ttgttataca
      361 atgtgccctg atttagtatt tgagatatta taaattattt ttattagagg ggagaaaagg
      421 tatgcaaagt ttattaatta aaggaaatga agctttagca gaaggtgcta tacgtggtgg
      481 ctgcagattt tatgcaggtt atccaataac accacaatca gaaatattag aaatgatggc
      541 atggagacaa aaagaagttg gaggagtatt tatccaaggt gaatcagaaa tagccagtgt
      601 aaatatggtt atgggagcaa gtgcacttgg agcaagagct atgacttctt catctggtcc
      661 aggattttca ttaaaacaag aaggtattgc atattgggta tcagctggtt tgccagcagt
      721 tgtagcatgt gtgcaaagat atggtatagg tgatggattt ataggagctg gtcaagataa
      781 ttattggcaa gcagtaaaag gcggtggaaa tggagattat catttaatcg tgttagcacc
      841 taatagtgtt caagaaaata tggatatggc atatgaatct tttgaattgg ctgaaaaata
      901 tatgcatcca gtacttatac tttctgatgc tacaataggt caaatggtag aaccttgtat
      961 agcaccacca atgaaagaat acgatatgga taaagctcca tgggctgcaa aaggaacacc
     1021 tgtaggagaa aaaaataaag taataactga tataacatat tttgaagacc aagatacatg
     1081 gcaaaaaggg tatatagaaa aaatgagaaa agttcaagaa gatgaacaaa gatgggaaga
     1141 gatagaaata gaagatgctg aagtggtatt tgtggcatac ggtatttcat caagaatagc
     1201 agaaggtgca gttagagatg ctagagcaaa aggaatgaaa ttaggtctaa taagaccaaa
     1261 aacattgtgg ccttacccaa gaaaagcatt taagaaatta agtgattcat tgaagggact
     1321 tgtaacagtt gaaatgaaca tgatggcaca gatgaaggaa gatgtaatta tagcatgtga
     1381 taatagattt ccagtatatt cagtagcaac agctcaatat atggcagaag taaatcaatt
     1441 aatagatttt gctcaacaga ttatagatgg aaaagctaag caagaggagg tattttaatt
     1501 atggctaaaa aagcaccagc aataatgatg tgtgaaaata tgttctgtgg aggctgtgga
     1561 cactctatag tcgatagaat tattggagaa gtattaacag aaatggatat atgtcaagat
     1621 acaattatat gttcagatat aggttgtaat catatgtttc aatttagtat gaatgtagat
     1681 gttgtcgttc cacctcatgg taaaatggga gcagcaatgg cagctatgaa aagagttaga
     1741 ccagataaat atatatttac aatagctgga gatggtggag cttatgcaat agggcttgga
     1801 gaaactatgt cagctgctac tagaaatgat aatgtaatat ttcttgttat gaacaataat
     1861 atgtttgcaa tgacaggagg gcaaatggct ccaactacta tagaaggtca atatacagct
     1921 tctactccag cgggtagaaa ttacgcagat catggttcaa catttgatgt tgtaaaagtt
     1981 atgggaaatt tagatatagc atatctagca agaggaacaa taactaaccc taaagaaata
     2041 aatacaacaa aaagatacat caaaaaagct ctagaaaaac aaaaagaaaa gaaaggtttt
     2101 tgcttaatag aagtgcttgt accatgtcca acaaactggg gattaaagcc agtagatgct
     2161 atgaagagaa ttgaaaatga acttgttcca aagtatccac ttggagaatt tattgattag
     2221 ggaggaaata atatgttgaa tgaaattata tgtgctggtt ttggaggaca gggagttctt
     2281 acagcaggaa aaattataat gtatgttgca tataaggcag aaaagaaaat tacatggttc
     2341 ccatcttatg gaaatgagat gaggggagga agtgcaaatt gcaatgttgt tgtaagtgat
     2401 gaaaaaattg caaatccttg tgtaaaaaat ccagatatac tattgggaat gaacagcgta
     2461 tcagtagata agtatgaaag tttaatgaaa aaaaatggtt atatgtttgt caatgaatca
     2521 ctagtaagta aggatagaaa gtacagagaa gacattaatg ttataaaagt acctgttact
     2581 gaaatagcac aaaatttagg caatgaaaaa gctgctaaca tttgcatgtt aggtgctatg
     2641 gtaaagagtt tggatttatt tacaaaagaa gaatttgaaa gtggtatgtg tgaatacttt
     2701 gaaaatcaag gcaaaggtaa attcaattct aaaaatgtag aagcatttga agctggatac
     2761 gactacgaat gaatatttta aatatttaaa ataattattg aaatacttat ttttatatgt
     2821 tagtatttaa ttaataaaat atagaacgtc gttctggaca ataggacgaa gtctttaggt
     2881 cggaatacaa gaacggcgtt ctgtatagta gaactagggg gtgttaaatt gaaagtttta
     2941 gtaataaatc ctggtggtac atccacaaaa atttctgttt tccaagatga aaatgaaatt
     3001 ttgaaaaaaa atataaccca tacaagagaa gatttaaaaa atttttctaa agtatttgat
     3061 gaatatgatt atagaaaaca actgatagta gatattttaa gtagtgagaa tcatagtata
     3121 aattcttttg acgcagtagt tggtcgtggt ggacttatga aggctataaa aggtggtacc
     3181 tatactgtga gtgaagaaat gattgaggat atgagaaatg aaataaatgg ggaacatgca
     3241 tcaaatttag gtgcattatt agctaaaact atagctgatg aaataggagt acaatcattt
     3301 gtagtagacc cagtttctgt agatgaattt gatgatgtat cgaggattac aggaatatca
     3361 gacatagaaa aagcaagttg gttacatgca ttaaaccata aagctgtttc aagaaaaatt
     3421 gcacaaaaac tagggggaaa atacgaggat tataacttta ttgtagctca tttaggttca
     3481 ggaatatcga ttgtagcaca taaacaaggc aaaatgattg atggaagtgg tggtaggtct
     3541 gatggtccat tttctccaga aaggagtggt ggattacttg catatcctct tatagaattg
     3601 tgttactccg gaaaatatac tcatgatgaa atggtcgcaa aagtaagttc gattggggga
     3661 atgtacgatt atttaggaac aaaggacatg atagaaattg aaaacagaat caaggagggt
     3721 gatgaaaagg caaaacttat aatggatgca tttatttatc aagtagcaaa agaaatttct
     3781 atgtatggag cttcactttc agggaatgta gatagagtta tcttgacagg agggatatct
     3841 cactcagaaa tggtagtcaa aggagtagca gaaaaagttt catatctagc tccaatagag
     3901 atagtaccag gagagatgga aatggaggca cttgctctag gggcacttag agtacttaat
     3961 ggagaagaaa tagcaaaaac ttattaatat ttaggagaaa gagtatgtta aaagatttta
     4021 ataatttatt agaagaggct aaaaagcttg aacctgttat tgtaagtgtt gctgttgcag
     4081 aagacaaaga agtattaaag gctgtgaaat cagcaacaga tttaaacatt ataaagccaa
     4141 ttcttgtagg agacaaggat aaaatagaaa gtatatcaag agaaattgga ttaaaaggtc
     4201 atgaaataat aggttgtcaa aatcccgatg aatcagttaa aaaagctgta gagatagtta
     4261 aaaataagga agctaaagtt cttatgaagg gattagtaaa tacaagtgtc tatatgagag
     4321 ctatattaaa taaagaaacg ggtcttagaa caggaagatt attaagtttg cttgcagtat
     4381 atgaattacc tcaatatcat aaacttcttt attgtactga tagtggaata aatgtatcgc
     4441 caaacttaaa tcaaaaaaaa gatataatga caaatgtact tcttactatg aaaagtatag
     4501 gcatggaaaa tcctaaaact gcgatactta ctgcaaatga aatggtagac cctaagatac
     4561 aatctacagt ggatgccaac ctacttgtag agatggaaaa atcgggagaa ataccaaagt
     4621 gtatagtgga aggtccaata gcatttgatg tagcatttga tgcacatgca gcagaacata
     4681 aaggtattaa aagtaatatt gctggtgatg ttgatttatt agtatttcca aatatagaag
     4741 cagggaatat attaggaaag tcatggctta gatttaataa agctaagtgg gcaggtatag
     4801 ttttaggagc aacaaatccg ataattttag gttcacgttc tgacacagct gaaataaaaa
     4861 taaattcaat agcattagcg tgcctagcat cacaataaga aattattatt aaggaggaaa
     4921 aagtgttatg aaatatgcaa aaagaatgga tatggtaaaa gcttcagcta taagagattc
     4981 tcaaaagaaa atagctcaga aggtagcaag tggtggaact gttatatctt ttgctgcagg
     5041 gcttcctgac ccaaatttat ttccaataga agagataagt gaaataactc aaaaagtact
     5101 taatgaaagg ggaaaatttg ccctagcata tggacctact aaaggtgaag atgagttgtt
     5161 agatctatta gtaaaaagaa tgaaagaaga agaaaatatt gatacaaaag cagaaaatat
     5221 tataataact actgggtcac aacaagggat agcattaagt gctatgattt tattagaaaa
     5281 gggagatgta gtagttacag aaaatccaag ttacttaggt gcaataaatg catttagacc
     5341 ttatgaatgt gattttttag gagtagatac agatgaagaa ggaatggtaa cagatgattt
     5401 agacaaatta ttatctgaaa attctaacat aaaggtgata tatgtaatac caacatttca
     5461 aaaccctaca ggaaaaacat ggtctttaca aagaagaaaa gattttatgg aagtcgtgaa
     5521 taaatatgat gtcatagtaa tagaggataa tccttatgga gaaataagat ttacaccaga
     5581 acctctacct acacttaaat ctcttgatac taaagataaa gtattgtacc ttgggtcatt
     5641 ttcaaaggta ttatgtccag gatttagagt tgcttggatg tgtggtaatg tagacatcat
     5701 agataaggca gaacttttaa aacaaggtat agatttacag tcaaatcaat tttcgcaagt
     5761 acaagtaata gaatttttaa agaaatacaa cttaaatgaa catatagaaa aaataagagc
     5821 agagtataag aaaagatgtc tgctaatgtt agatgctatg aagaaacatt tcccagagga
     5881 agctaaatat acagaaccag atggaggaat gtttatatgg gttgaacttc ctgaaagtat
     5941 aaatgtagat gatttattag ataaagccat tgatgctgga gttgcttatg tttctggaga
     6001 gtccttcttt gcaaataatg gtccaaaaaa tactatgaga cttaactata caacaatgtc
     6061 agaagaacaa atcgttaaag gtgttgagat acttgcagaa gttataaaaa aagaactcaa
     6121 ttatgcttgt gaataaatat aaaaagtgag ggtttaaata tgggagaaaa agaaaacggt
     6181 ataaagtttg gaaagttaat gtttcaactt gttattatag caatatcttg ggaattagtt
     6241 tatattatac catttatcca atatacatta tatgatccaa tcctaaaagc attagaatgt
     6301 acaaacactc aactaggttt tctacttaca atttatgggt taggtaatat atttggagct
     6361 cctataggag gctggttagc agatagattt gattatagaa agataatttt aggttctgta
     6421 tttcttaatg gagtagtatc atttttattt gcatttaata tgaactatcc atttgcagtt
     6481 gctacttggg taggttgtgc aatcacatca ttagttatga actatccttc tatggtaaaa
     6541 atacttagag ttataggaaa agataatcaa ggtaaggttt atggatttaa tgaagctatg
     6601 gttggagttt ctggagtagt aatgggagca atattcttat atatttatac atgctttgca
     6661 actcctacat tgggtatgag atgggtaatg atatctttag gaatattatc aatagcaatg
     6721 tgtccagtat tatggtttgt aattaaagat gtagatatta aagaagaaag agaagaagca
     6781 ggtgaaaaaa tgtctgctgg tgattttata acagtactaa aatcaccaaa tacttggcta
     6841 gtaggaataa gtatattctg tgtatattca ttcacagtta caatgtctta ttttacacca
     6901 tatataacat ctgtacttgg aggttctgta gcattatctg gtacattagc aataataaga
     6961 cagcatggtc taaaattatt tggagcacca tttggaggat attgtgcaga caaattaaaa
     7021 tctcctacaa aagtactttt accaatatat gtatttggta tagcagtaat agttttattc
     7081 ctagtattac ctgcatcaac tccaatgact atattcatag ctttaacatt tgtagtaggt
     7141 atattaggtt acatgggaaa aggtatatat tatgcagtac aagatgaagt aaaagttcca
     7201 gttaaatatt cagcaactac tataggtata gctgcagctt taggattttc accagatgta
     7261 ttccaatttg cattaatagg acactggata gatacttatg gcaataaagg ttacacatat
     7321 acatttatat tccaaatagc aattctagtt attggaatac tatcttgttt atatatatta
     7381 aaggttaaaa agagaagagc aagtaaatta gaagcttaaa tattcaattt ggtaattaag
     7441 tatatataaa tacaatactt attttgatga atgtgattgt ataaataaca taaaaaatat
     7501 caatagatta agttttataa taatgtttag agaataaagt ataaaaatca aaatgttatc
     7561 aagttttcta ggttttaaca taaagttacc cctgaaggtg taaaattgag tggcaattat
     7621 tatctatttt cttacctaca taaacctatt taatttaaat tctaagagat tttcaaaata
     7681 gatggtagtt gtactcaatt ttaaacctca taataaatac gaaaatttaa aatctaacta
     7741 gatactattt aattatatat taataaatat atattgtact atttatataa taataatttc
     7801 aggcttttca ttatatgtat tccaatttat attgtttgat tattattcaa acacacgtcg
     7861 ttaaaaattt tataaattta tgtttagata tgattaatac tcttgatttt agaaataaat
     7921 atttgtagat tgtagattaa agataatata aataaataga aggtagattt tttagatata
     7981 ttaatataaa gattatataa aatattgaaa tatagctgtt taaattattg tctataaata
     8041 taaaatgaat ggtggtgagt taagtgaccg atttagttaa caccattaat ggaattgttt
     8101 ggtcacccgt attagttgca atgtgtttgt gtataggatt atatttttca ttgaaattaa
     8161 aactttttca aataagagat gtaaaagaaa tgtttactct cttgcttgaa ggagatagtt
     8221 ctcatgttgg tatatcatca tttcaaggtt ttgctacatc tatggctgga agaataggga
     8281 ctggaaatat agctggtgta gctgtagcaa tagcaatggg aggtccagga gcattagtat
     8341 ggatgtgtat aatggctaca ttaggttcag caactgcatt tgtagaatct actttagctc
     8401 aaatatataa agatgagcat gatgggcagt atagaggagg acctccatat tattttgaaa
     8461 aaggtcttgg gtggaaaaag tgttcagttt tattttcaat agttgcaata ataagttata
     8521 cattttttat gcctggtaca caatcaaaca ctttatatct agcaatgaat caagcatttg
     8581 gattttcaaa aatgacaata gcagtagcat cttctattgc attagcgata ataatttttg
     8641 gaggaattaa aaggattggt ttatttgctg aaaaagtagt acctataatg ggtggagcat
     8701 accttattat gacattagta ataattataa caaatattag tttagttcca gaggctattg
     8761 taacaatgtt taaatctgca tttggatttg gttcagtttt tggaggaatg ctaggttcag
     8821 caatatcttg gggagtaaaa agaggtatat actcaaatga agcaggagaa ggttctggga
     8881 cttatggtgc ggcagcagca gaagtaagtc atccagccaa acaaggatta gttcaagcat
     8941 tttctgttta tgtagacaca ttattaatat gtatggctac aggtattatg attgtaataa
     9001 caggaatgta taatgttaca ggtgtttgca cagcactacc aggtgtagaa ccgggaccac
     9061 aatatgtaca gagtgcaata aatacattac atccacaatt aggtgatatt tttatagcaa
     9121 tagttttatt attatttaca tttacaactt tattagcctt ctcatatatg atggagacta
     9181 atgtatctta cttagtaact agaattaaag cagaaaaaca tgaaaagtac ataattaatg
     9241 tctgtagatt tatgcttata gcatgtgcag caatgtgttg ttttatagaa ccacttacaa
     9301 gctgggcatt aggtgatata tgtataggtg tattaacata tataaattta attgccatct
     9361 ttacactaaa aaaaccagct ataaaggcat attatgatta tataagacag aagaagatgg
     9421 gaataaaaag gagtgataga acttttaacc ctatagagtt agatataaga aatgctgagt
     9481 tttgggaaaa tagatactta aaagaggata agcatgagtt aaattaaagt tataataaaa
     9541 tataaaaatt actaaataaa tttagtggga ggtaagattg gtatgaaaga aagaataata
     9601 caagtggctg attcaaagaa agaaaaaatt ctagggctat gtcaatcttt atatgatgaa
     9661 ccagaaattg ctttgcaaga atataaatct gctaaaaaaa tatcagaatt tctaagagaa
     9721 gagggttttg atgttgagga aaacttggca ggtatggcta cagcttttaa agcaaccaag
     9781 aaaaatggag atggtcctaa aatagcattt atagcagaat atgatgctct tccaggcaat
     9841 ggacatgcat gtggacatca tttaattgca tctatgggag ttggagctgg aatagctctt
     9901 tcaagtatac tggatactta caagggagaa gtaagtataa ttggaacacc tgctgaagaa
     9961 actggagatg gaaagcctta cttgatagaa catggtgtat ttgatggcta tgatgcagca
    10021 atgatgatac atccaaattc aaaaacttgt gtaacacctg aaattatagc aataggaggt
    10081 ctagatttca tatttactgg aaaagcatct catgcaggag ctaagccata taacggaata
    10141 aatgctttag atgcagtagt actactatat aataatataa atgcactaag acaacaatta
    10201 gtagatggaa cgagaataca tggtattata ttagaagcag gaactgctgc aaatgtaata
    10261 cctgatatgg gaaaagtaag attagagata agggcaaaag aacaaaatta ctttgatgaa
    10321 gtagtagaaa aagtgaaaaa ttgtgctaga ggtgctgcaa tagccactgg atgtgaactt
    10381 gaattttatc actttgaacc aacttgccaa ggattaaatg aaaataaagt tttggtagat
    10441 atatttacaa agattatgga ggaatttgga atatatgaag atgaacaagt aatgttaggt
    10501 tcaacagata tgggaaattt aagtcaaata atgccatgta tacatccttt gatgaagttt
    10561 tctgaaaatg gagaagaact acatacaaaa gaatttttgg aggcttctat aaattcatat
    10621 gcaaaagata gagtaattga tggtataaaa atattagctt taacaggatt taatttattt
    10681 gaaaatccag aattattgaa aaaaatgaag gaagagtaga acttagacta tttttaatta
    10741 tactctttaa aataagaaga gtgtctcaaa atgaactttt tagtttatga gacactcttt
    10801 tttgtatgaa attaaatata tttttattat tttaaacatg aaaaagaggc ttatctgttt
    10861 ttatatgacc ttttttaact tgtagcacaa aatatcatga atgattacaa ttcgaatgat
    10921 aaaatcgatt ataagatata taggaggggt acatatggaa actttagaag taagaaatat
    10981 tgaattgagt ggtacaaatt atgagatagg atacagacta ggagaattgg ttgctaatat
    11041 gccagagata atagaaggtc agattaataa atctaatctg gtaagcaaaa aagaagaaaa
    11101 agagatgata gagttgtttg ataaatattg tccaggtctt aatgaagaat tgcaaggttt
    11161 tgctgatgca attcaagtga attgtaatca aattttatat tatactatga cttatcttaa
    11221 accaggttgt agtcaagttg cattagcacc agaattaact gaaaatgggc atgtactatt
    11281 tgcacgtaat ttcgatttct cacacaatat ggaggatttt gttttatgta aaactaaggt
    11341 aaatggaaaa tatgcacata taggtactac cattatgcaa tttggaagag gtgaaggtat
    11401 gaatgaatgt ggcttaggtg taagtcagtc atcatgtggt attcctgtag gcaattcaga
    11461 tggtttaaga aaaccagcaa ttgtaggttt acagttttgg gctgttatta gatatttgtt
    11521 agaaaattgc aaagatgtag atgaagcatt agaatattta aaagatatgc caattgcata
    11581 caatattaat ttattgcttg cagataagtc tggaaacatt gcacttgtgg agactttaga
    11641 tgggaaaaaa gaagtaaata taatcaacag tttagaaagt aaaagagaat attttttaca
    11701 ctctactaat catacacata ttgataaact tcataaatta gatccacaat caatgaaaaa
    11761 ttctattcat agatataaat taattaaaga atatataaat aagtctaaaa aaattggaga
    11821 gaaagagtta atgaatttat tgtcttcaaa atatccaaat ggcttatttt gtaattacta
    11881 taatgatttt tttggaactt taaaaagtat agttatggat ttaaatatag gaaagtttaa
    11941 tattttatgg ggtggactag agaataaatg ggagagctac tatttaaaaa atgacattaa
    12001 atctataaca cagaggataa acataaatat tgaaaaagca ccatctgact tttttgattt
    12061 cattgaataa aaaatgtatt caattattct aaataattag gatatataaa tcaactaaaa
    12121 gtttttattt atcgtattga attagttata atattttagt atagtaaaaa atagtgctat
    12181 tttacatatt taaagagttt aagtaaaaga gaggtgccat attggattac ttaaagtatt
    12241 ttgaaaaagc tgtaatgtat attgagaata atttacatga aaatataact gtaaatgatg
    12301 tggcaaaaga aactggatat tcttattatc atttaacacg cttatttaag tctatgtttg
    12361 gtgagagtgt aggaagttat ataaaaaaaa gacgattagt aagtagtaca aaagaacttt
    12421 tatatagtga taaaaaagtg atagatatag ctattggtag tggatttgaa tcttcagaag
    12481 catttagcag agcatttaaa tctatataca aggttagccc tatagaatat agaaaaaata
    12541 gaattgatgt atttgttggt aaaaagaaaa aattagagtt agattttatg agacatcttg
    12601 ttggaaatat aacgattaaa ccagttataa aagaaataga aaaaataaag gtaataggta
    12661 taaaggataa agtaatttta gaaaataatt cattacctga cttgtgggag aaattcagaa
    12721 aagtacatca tattgtccca aatactttgc catcaaagag agtttttgga atctgtgaag
    12781 caatctctga aatacaccta gttagtgaaa gtatggaatt taatgagata ataggacttg
    12841 aagttaatag ttatgatgtg attccaaatt catttgtttc aaaaactatt aaggaaggaa
    12901 agtatgctat atttactcat actggtaact tagataattt agacaaaact tatgaatata
    12961 tatggggtac ttggttttta agttcaaaag aagaacttga tataagagat gattttgaag
    13021 tatatgatga acgattttta ggtcctagta atgtgaactc acaaattgat atttatatac
    13081 ctataaaata atctataaga aaactaaaaa ataatttgat taagaaatag atgttcgtaa
    13141 aagagcatct attttttatt tttataagat tta
//
LOCUS       DAKBNN010000107        13145 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000107 DAKBNN010000000
VERSION     DAKBNN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.108"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            8..811
                     /gene="larE"
                     /locus_tag="OUB02_002467"
     CDS             8..811
                     /gene="larE"
                     /locus_tag="OUB02_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HCT8886217.1"
                     /translation="MKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKDTLGEN
                     VIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYHCKKYI
                     FSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEEIRSLS
                     KTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRMHGDIA
                     RIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            866..2014
                     /locus_tag="OUB02_002468"
     CDS             866..2014
                     /locus_tag="OUB02_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCT8886218.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            2158..3546
                     /locus_tag="OUB02_002469"
     CDS             2158..3546
                     /locus_tag="OUB02_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8886219.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            3575..4273
                     /locus_tag="OUB02_002470"
     CDS             3575..4273
                     /locus_tag="OUB02_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886220.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            4276..5709
                     /locus_tag="OUB02_002471"
     CDS             4276..5709
                     /locus_tag="OUB02_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8886221.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            5731..6468
                     /gene="truA"
                     /locus_tag="OUB02_002472"
     CDS             5731..6468
                     /gene="truA"
                     /locus_tag="OUB02_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCT8886222.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    6579..6786
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7039..8238
                     /locus_tag="OUB02_002473"
     CDS             7039..8238
                     /locus_tag="OUB02_002473"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCT8886223.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWNIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            8485..9531
                     /locus_tag="OUB02_002474"
                     /pseudo
     CDS             8485..9531
                     /locus_tag="OUB02_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
     gene            9639..10802
                     /locus_tag="OUB02_002475"
     CDS             9639..10802
                     /locus_tag="OUB02_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8886224.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNHSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(10891..11271)
                     /locus_tag="OUB02_002476"
     CDS             complement(10891..11271)
                     /locus_tag="OUB02_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HCT8886225.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(11274..11933)
                     /locus_tag="OUB02_002477"
     CDS             complement(11274..11933)
                     /locus_tag="OUB02_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCT8886226.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(12039..13013)
                     /locus_tag="OUB02_002478"
     CDS             complement(12039..13013)
                     /locus_tag="OUB02_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HCT8886227.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
BASE COUNT         5255 a         1340 c         2218 g         4332 t
ORIGIN      
        1 aaattttgtg aaggaaaaag aaaaattaga taaattaaaa aaaatgttac ttgaattagg
       61 cagtgttgta gttgcttatt ctggtggagt agatagtaat tttttgctta aggttgcaaa
      121 agatacatta ggtgaaaatg ttatagctgt gactattcat gcaatgatgc attcaagtag
      181 ggaaattgaa gaggctaaac aatatactca aaattttggt gttaaacata tcatattaaa
      241 tatagaaaac tttgatttaa aagaatttaa agaaaatggt gttgatagat gttatcattg
      301 taaaaaatat atattctcta aaataaaaga ggtagcaaaa gaacataata taaagtacat
      361 agtagatgga acaaatatag atgatttagg agactataga cctgggctta aggcattgag
      421 tgaattagga gtaattagtc cattaaaaga tagtagttta aaaaaagaag aaattaggtc
      481 gctttcaaaa acattaggat taaatacttt taataaacca tcatttgcat gtcttgcgag
      541 taggatacct tatggagttg aaataacaga tgaaaattta agaatagtag aaaagagtga
      601 agaatatcta tccaatttag gtttttctca atttagagtg agaatgcatg gagatatagc
      661 tagaatagaa gttggacagg aagaactagg taaatttttt gaaaataaca attttaataa
      721 agttgatact aaattaaaaa tatttggctt taaatatgtt acactagata tgtcaggata
      781 taaaatggga agtatgaatt taaatgtgta gattatttta aaaataaata agttttatag
      841 ttaatataag gaaacaggag gttttatgaa aaattatacg ttaatatcgc catgtttttt
      901 tggaatggaa aaaatgctgg ctagagagat tactaattta ggatatgaaa taataaaaac
      961 agaagatgga agaataacat ataaaactga tgagtttgga atagcaaagt caaacatgtg
     1021 gttgagatgt gctgaaagag tacatcttaa aattgctgaa tttgaggcaa aaagctttga
     1081 tgaattattt gaaaatacaa aaagaataaa ctggtctaga tatatacctt atggagctca
     1141 atttcctata tcaaaagctt cctctataaa atcaaaatta tacagtacac cagatgtaca
     1201 agccatagta aaaaaggcta tagtagaaag cttaaagaaa agttatttgg aggatggttt
     1261 gcttaaagaa gataaggaga aataccctat ttttgtattt atacacaaag acaaagtaac
     1321 catttctata gatacaacag gagatgcttt gcataagaga ggatatagag aaaaggcaaa
     1381 taaagctcca ataagagaga ctttagctgc tgggcttatt tatttaactc cctggaaagc
     1441 aggtagagtt ttagtagacc ctatgtgtgg ttctggtact atattgattg aagctgctat
     1501 gattggtata aatatggcac ctggcttaaa tagagagttt atatcagaaa agtggagaac
     1561 tcttgataag aaaatatggt gggatgtaag aaaagatgct tttaataaga tagataatga
     1621 atctaaattt aagatttatg gatatgatat agatgaagaa tctatagata tagctagaga
     1681 aaatgctgaa atagcaggtg tagatgaata tatagagttt aatgtaggag atgcaactca
     1741 atttaaaagt gaagatgagt ttggatttat aatcactaat cctccttacg gagaaagatt
     1801 agaagataaa gacagtgtta aacagttgta taaggagctt ggttatgcat ttagaaaatt
     1861 aaaaaattgg tcgtattatt tgataacttc ttatgaagac tttgaatatg agtttgggca
     1921 aaaagcagat aaaaaaagaa aattgtataa tggtatgtta aaaactaact ttttccaata
     1981 tccgggtcct aagcctccta gaaataataa ataagtttta catgatttat ttgtaaaata
     2041 taaaatttta ttaaaataag taaaacatta tgatgccttg aaacgtctaa ttattgtgag
     2101 gagatttttg tgaacaaatt taaagattta aagatttata ggattaggag gagttaaatg
     2161 aaagtgttaa agcttattac tgtagtagcc ctgatgatgt tatttgttac tggatgtaat
     2221 ataaaaactg aatctccaga acaattagcg aagatacctg actatgatga tacaaaaaaa
     2281 gtattgtatg atggtattga ccagttacta aaaccagatt catctatgat attgccttct
     2341 aatacaaaag aagtaggaaa aataaataag gtagatttaa acagcgatgg aattgatgaa
     2401 ttagtagtat ttgaacaaaa agaagattta agtaataatg tgagtcaggt agggtttgta
     2461 accttgagtt acaatggaga aaaatacgta cttggagacc attttttaga aaatggcgaa
     2521 tcaatagaat atgctaattt ttatgatttg gactcagatg gatataagga agttatctta
     2581 ttggttaaat ccaaagacaa aacgaatatg catatatata aagtagtaga taatgaaata
     2641 acaaaaatat atgacttgga tgcatcatgg cttcaaaata aagaagattt taatgacatg
     2701 aaagtaaaaa ttggatatat agatggagat gacaagttag acatattgat gcttcatttg
     2761 aataataaga ctaatgaaat gtttgcaagt gttgcaaatt ttgatgggaa aatgaaaatc
     2821 aaagattctg ttaagtttga aaatgtaaaa aacttaagtg aattatatat aacattaggt
     2881 aatgttgctt catctgttgg aaattcttct gcagctataa aaggtattgt acttgatatt
     2941 cctatattga aagataataa ctatattacg cagatacttt atatgaaaga tgggaaaatt
     3001 aacaaagctt ttagtgatta tgataaaact attactaaat cttattatat acctgtagat
     3061 gatattgaca aagataagat tatagaaata cctatagttg caggcagtac aggtaataat
     3121 aaaaatactt atagttctaa atcatctgca actataagtt ggtatagatg gaatggaaaa
     3181 gaaggtgctg actcaagttt aatatttaca agtcagatat attataatta taaatataat
     3241 tttaaattat ttattccaaa taatttattt gataaaatac ttgttgaaca ggaatttgtt
     3301 ggagaaaaag cagtatttaa attttactat tttgactatt cagaccgtaa gaatttattt
     3361 aatatagtag tagagagtaa aaataaactt gaagatagaa aaaatacagg tactcaaaat
     3421 tctattgtac ttcaagagag cgatgaatat actttcttac tagttgtaaa caatgcaaat
     3481 gaaatggata agctagatat gactatagac gcattaaaag aatatttctc attaatatat
     3541 ggttaatatt atactcaatt ggaggtttta ttttatgaag gaaaagatac ttatactaga
     3601 agatgaaatt ggtattagaa gttttgttag tataaactta aaaagggaag gctatgaaat
     3661 agttgaggca ggtactggaa gagaagctat agaaaaaatg acaacagaaa aggatattac
     3721 aatagctctt ttagatgtaa tgcttccaga tataagtggt atagaagtat gtaaatttat
     3781 aagggagaat tttgaccaag taggtataat aatgcttact gctaaagctc aagaagatga
     3841 taaaatagaa ggatttatct caggagcaga tgattacatc ataaaaccat ttagtataaa
     3901 agagctttta gtcagagttt ctgctcttct tagaagagta gcaaaggatg attcaagtgt
     3961 taagtcgagt gagatagttt ctccaccatt tatacttgat atagacaaaa gaaaattatt
     4021 taaaaatgga aaggaaattg agcttacacc aactgaattt tcaatagtga aatatctaat
     4081 atcaaatgct aagcagtcgt taagtagaga ccagatatta gacgaagtat ggggaactaa
     4141 ctatttatat gattttaaaa tagtagatgt aaatataaga aggataagaa ataaaataga
     4201 agatgaccca tcaaaaccaa aatatataca aactatttgg ggttatggat actgttttag
     4261 aaaggaagaa taatagtgtt acattttgaa ggtctgagaa aaaaagtaat aaaaaactat
     4321 tttataataa ttattataat ggttactctg tttgaaggat tgttcatgtt ttacatacaa
     4381 aactactatt atgattctgt caaacagcta cttgaatctg aaataaaata tgctgatgaa
     4441 tataatgcta ttacaatgga aactacaagt tttgagaaaa aagtaaaaaa tatatttgat
     4501 aaacaaccct taactaaaaa ttcagaattt ggaatttcaa taatagataa agataaaaat
     4561 attatattag accaatatgg ttttaaaagt aaagaaaaag caaactatga agatgttaac
     4621 aatgcattaa aagatataaa aactaaaaat ttaacacctt atacttatag aataccagat
     4681 acaggagagc atgttatgag tatctcctta ccacttaagg taaataatat aatagaaggt
     4741 gttgttagat atacagtatc attagatgct attgataatg caatattaaa gcaagctaca
     4801 tggcttatat tagcaggaat atttatactt attatagcta tactaataag tcttaaattt
     4861 gctgagactc ttataaaacc attacgagag ctaaagaaat ttgcaaatga attggctgta
     4921 ggaaattata atataaaatt agaaaagatg aagattgtag atgatgaaat tggtgattta
     4981 gcacagacat ttgaacacat ggcacatgag attgacaaaa gtgaaaaatt aaaagaggaa
     5041 tttatatcat ctgtatctca tgaactaagg actcctctta catctataaa ggggtggagt
     5101 gaaaccttag gttatgaaag tataacaaga gaagaactag acttaggttt aggcattata
     5161 caagatgaaa cagaaagact tataaaacta gttgaagaat tattagactt ttcaaggctt
     5221 tcttcagaca ggataaaact tcatgttgat atagttgatg ttgaaggttt aattgtaggt
     5281 gttgtaaatc aacttaaagt taaagcagct gaaaaagata tatcactttt atttgaattt
     5341 gaaaatgaat ttatagagaa cattcaagga gataaaaata gattaagaca ggttctcata
     5401 aatttaatac aaaattcatt taaatttacg agtcaaggtg gttacataaa agttgtagct
     5461 tctcaagatg aagagataac aaccatatca gtagaggata atggttcagg tatagaaaag
     5521 caaaatttaa ataaagtgtt agataaattt ttccaagaag attataataa ggctggtagt
     5581 ggattaggac tagcaattag taatgaaatt gtaaaacttc atggtggaag aatgaaaata
     5641 gaaagtgaaa agaatgttgg aacaaaaata acttttaata taaaaaataa atttgctaaa
     5701 caagcataaa ttcttagaaa gaggattaag atgagaaaca taaaaatgat agtagcatac
     5761 gatggttcaa gatataaagg atatcaaaaa ttaggtgaca ataatatgac tattcaagaa
     5821 aaacttgaaa atgttttaag taaaatgact aatgaaacag tggagataat aggttctgga
     5881 agaactgata tgggagctca tgcaagagga caagtggtaa attttagaac taattgtatg
     5941 gattctttgg ataaaattca aaaatattta tatgaatatt taccagaaga tatagttgta
     6001 aaaactgttg aagaagttga tgaaagattt cattcaagat acaatgtaaa gtcaaagact
     6061 tatatgtata aaattgacaa taataagtat cataatcctt ttataagaaa atatgctact
     6121 catgtaagta aaaaattaga tttagataga atgagaaaag caagtgagta tttagtagga
     6181 gaacatgatt ttactagttt tgcttcttct aaatctaaga aaaaatcaaa tgtaagagaa
     6241 atttattcta taaatataaa agaagatgat aatgttatag aaatatatgt agaaggaaat
     6301 ggatttttgt ataacatggt aaggataata gtaggtgctt tgatagatgt tggtctaaaa
     6361 agaagagctc cacaagatat taagtatatg cttgaatcaa aagatagatg tcaatcatca
     6421 gatactgcac cagctaaagg actatgtctt tggaaagtaa catattaatc aattatataa
     6481 aactctcttt aaaataaaaa taaaataaat tatatagttg acataaatgg aaaaataaac
     6541 tattatatat tttataagag agatataaaa ctttatattt aaatcctgtg agaaagagag
     6601 taacttctat gtgttttata gagagctgag gatggtggaa gcttggcaaa taagtattag
     6661 tgaaaagaac tttggaggat actaattgaa tttagtatgt tagtaggtaa tctcgcctta
     6721 agagaaagag tggataagtt tatcttatca attagagtgg taacgcggat atatttcgtc
     6781 tcttggtttt tccaagaggc gttttttaat atatttaatt attagtgttt atctggtatg
     6841 ttgaacattt actaattgat tagctaagtt tatcaataag agtggtaccg cggatatttc
     6901 gcctcttagt attttaaaat actaaggggt ttttttagta ttaaaatgat tttatgtgag
     6961 tctaaattct cagagtcatt caatattaag aatttctata aaataaaaat tataatttaa
     7021 gattgggagg aattaaaaat gaaggaaaaa gtagtattag catattcagg aggacttgac
     7081 acatcaatca taataccttg gttaaaggaa aattatgaag atatagatgt tatagctgtt
     7141 tgtggaaatg taggtcagga agataaaatg gaagatgtat atgaaaaagc tttacaaagt
     7201 ggagcatcaa aggcatatgt ggatgatata agtgaagaat ttgtaactga aactatattt
     7261 aaagccgtaa aagcagaggc taaatatgaa ggaaaatact tattaggaac ttctttagca
     7321 agacctataa tagctaaaaa attagttgaa gttgctcata aagaaggtgc aaaatatata
     7381 tgccatggat gtacaggaaa aggtaatgac caagttagat ttgaagctac aattgcagct
     7441 cttgacccga ctataaaagt tattgcacct tggagaattt ggaatataaa atcaagagaa
     7501 gatgctatag actatgctga aaaacataat ataaaggtaa cagcaactaa agctaaaata
     7561 tattcagtag atgctaacct ttggcatgtt tcaacagaag gtggagatat agagcaccta
     7621 gaaaatgaac ataaaaaaga tgtttataaa caatgtgtag acccagaaga tgcatgtgat
     7681 gtagctgaat atgtagaagt ttactttgaa aaaggtgtac ctaaaaaggt aaatggcgaa
     7741 gaattatctc cagttgcact gattcataag ttaaatgagt tgggttgtaa acatggaatt
     7801 ggtgttatag atatagttga gaatagactt gttggtatga aatcaagagg tatatatgag
     7861 actccaggag gaacaatcct ttatgaagca cataatattt tagaaagtgc tactttagac
     7921 aaagatacat tacatttcaa acaaatggta tcttataaat acggagaact tatttacaat
     7981 ggactatggt attgtaaatt aagagaatct atagatgcat ttatggaaca aactcaagat
     8041 aatgttacag gaacagtaaa agttaaactt tacaaaggaa atataaaacc tgctggaata
     8101 tttactgaaa atgctcttta tgatgaagga atatcttctt ttggaaatag tgaactttat
     8161 gaccacaaag atgcagaagg atttattaat ttattcactt taccattaaa aatcagagct
     8221 atgaaagctg gaaaataata aaataaatta tttaagagtg tataaaaact atttttaggt
     8281 ttttatatgc tcttttttta tttgaattta ttaaattata cagtaaatag tataaaaaaa
     8341 tatattatag ttccaaatgg cttaataatg tgaaaatata agacctaaaa tataaatttt
     8401 ataaatacaa tagttattga ctctctcatt gtaagagggt ttatactgaa tctaatgaaa
     8461 atttatagtg aggagataga ttatatgaga aaaataattg aaattaaaca tcattcacat
     8521 gattatgagt gtatgtggaa tggtatagaa gatttatata taaataaaac cggtgaatgc
     8581 cttccaaacg attttttctt tttactgtca ggttttggtt cattttgtta tatgaaaaca
     8641 aataaagctg atttgaaaag aatggtagct ttgggagatg gaaggacaaa acagatgtat
     8701 aaatttttag caccaattgt tggatttgaa tacaaacatc atgagtttaa aaagtatgag
     8761 caagcattaa agaaagcaaa atctgaaatt gatgatggat atccagttat tttaggagct
     8821 ttagatatgt attatctacc atattataag aagctatatc ataaagaaca cattcctttt
     8881 cattatatat taatggtagg atatgatgat gaaggaatat atctgtatga ttgtggtaga
     8941 acagaattat tacatctttc ttatgataaa ttacgtgaaa gcatgaattg aaattcccct
     9001 ggattaagca aagaaaatac tatttgcaca ataagaatga atacttcaaa gaataaatat
     9061 aacattgcta gagaagctat tgctataaag cgtgatatgt ttttaaatcc acctaaagga
     9121 tttttgggat ataaaggttt tgaaaagttt attaaagagt tgccacagtg gaacactgaa
     9181 ttggataaag atgattatga taagattttg accaatatgg ttatgttttt tggaacagta
     9241 ccaacaattc caaatgcaat caaaggaatt aaagaaccag atactgttca atttaaaggt
     9301 ggatttgata aaatgagtag aatgctcaac agtattgggg aggaatacaa aaacgactct
     9361 tttatacaag tagctgatat ttttgataaa actgctatta taattgagaa aatatccaat
     9421 attataatag attacttaac tcaaacatgt gatgatacag gacaacttcc gttattgttc
     9481 tctgaggtct tagagcatat gaaaacagga tatttaatct tggatgtata aaaaatatat
     9541 tgttgaccct aacattacgt aatagtgtaa tatgaaaata tagcttgaat atattagtag
     9601 gtattgatgg ctgtaaaatt aaaatttgag gtgtggatat gagaacagtg aaacaagttt
     9661 ctgatttaac tggaataagt gtgagggcat tacattatta tgatgaaata ggtcttttaa
     9721 aaccaaataa aattacagat gcaggatata gactttatga tgatgaatct attaaaactc
     9781 ttcaacaaat tttatttttt aaggagattg atattccttt aagagaggtt aaggaaataa
     9841 tgtcaagcca atattttgat aaggttgaag ctctaaaaaa tcaaaaaaaa ttacttattt
     9901 taaaacgaaa gagactggat gaattgatag agcttataaa tcaaactcta agaggagaag
     9961 gaaatattga ttttaaagag tttgatatga gcgagtattt tgatgtttta gaagaattta
    10021 aaagagagca taggaataag gtaattaaaa tttatggtag tgtagaaaaa tacaatgaat
    10081 atatagaaag agttaagtct aatgaagaga aaattgctaa aatggctata aaacaatatg
    10141 gaactattga gaagtttgcc caagctataa aaacaaattt tagcagtgat atattgaatt
    10201 taggagaaaa atttgataga tataaaaatg attgtttaaa agaaaaacat ccaaaattaa
    10261 aagaattata tagaaagctt gtagaagatt taagtaggaa tcattcttca acagaccttc
    10321 aagaaatagc aaaagagata acagatatat ctaaaaaaga ttatgaaatt ttctctatgg
    10381 atactggtga tgacaactgg tattatatgg ttcaaaatta tctagtaaat cctatgtgga
    10441 tagaagaagt agataaaaag tatggaagtg gagcaggtaa gtttattgga caagtactaa
    10501 agacttatct aagagataga aaaccaaaga taaatacatt gtatgaaaaa cttgtagaag
    10561 atttgagtag agattgttct tcaagggaag ttcaaagcat tgttgaagaa atagacaatg
    10621 aaatgaaaag aagtaatgaa ttttataaaa tagacaatgg tgaaagatac tttgattata
    10681 tgtcagagct ttatttacaa gattctaact atataaaagt aactgataag aattatggtg
    10741 atggtgcatc taagtttatt ggagaagctt ttaagattta ttttgataat aataattgtt
    10801 gaaaatttgg aagtaaaaaa tatgtaagtt ttataaaagc acaaaaataa ggatgtagat
    10861 tagctccatc tatatcctta tttaaagtta ttaaaatata tagtttacat caaacttaac
    10921 actttcaata atatttccca aatatgtttt tatactttca acttcttcta aagattttaa
    10981 tttattttca gtataaattg ctttgtccag aatattaaac cttaatccta tttcatcaaa
    11041 gtactcatga tttaaaactt tgtaccaatg gtttttttct ttattccaac tcgtagatat
    11101 tgaatcagct tctttttgag ctttttccca atcttcattt tctatagatt tttctaaaac
    11161 agatatattg tctttatatc cttctgtaaa atcatatagt ttattgttaa cgtaaaatcc
    11221 aaataataca aataagatag tccatagtat aacaaatgtt gtagatttca atattaatca
    11281 ccctttttat tttgaataaa tattttgtca ttttcatcta aaacacaaat aagaacatct
    11341 ttaaaagttt ctatgtgatt agattttaaa atacccatta accagttagt atctttttga
    11401 gctatgtcta gatttttatt tattattcta ccatccagta taagtgggat aggtaaatga
    11461 ttaaaagctc ttggaggagt actattgtag cttgatgctg gtacaacact tagattacca
    11521 tcagtctcca aaatagcata ttgtacatct tttaggttaa agtatccttg aatcctaagt
    11581 tgttctaata attcatctat actaattctt tcttttttaa gctctttata tacaattttt
    11641 cctttatcta ttaatacaga aggtttacct gataaaaatg aacttaattt tctgcttttt
    11701 aatgataaga atgaaattag agtctgcatg attacaagtc cagatattgc tgcaacacca
    11761 tttatgattg gtatattatt attttccatt gggacagatg caacttctgc aattaatagt
    11821 gttataacta aatcaaaaca gttcatttca gctatttcac cttttcccat gactctaagt
    11881 gcaattagca ctgttatgta taatataata cttcttatta atactactgt catatctatc
    11941 aattactcct ttaaaaaata tttacactta gttagtatat cctatataaa aaatttaatt
    12001 caaagtataa aaaaccttaa ataattggat tcaactattt aaggtttttt tgataatttt
    12061 aatattaaat tttttatgga ttttctgtta cttttattta ttttaataac agattcaaca
    12121 aaactctttt tatctcctaa ttttaagata aaatcaggat attcttcaaa ttcattgagt
    12181 tttattaatt tgaaagaaga agatagattt ttaaagttaa taattttaga gttattcact
    12241 aatacacttc tttttttaaa ataaatttct tttttactta tatttagtat agttaatatt
    12301 tgtttaagat atattattat atcttctgga gttctatcaa atttgagttc aaaattttca
    12361 tcttcaaagt agggaatata atataaaggt gttttgagag caggtaattt attatataat
    12421 tcttcaagaa tatcgatatt tttaggggag ttaaagaaaa agcatttaca cacattcatt
    12481 aaatccacag catctgaaag tgagttatgt ttaatatctc cttctacaga aaaaattttt
    12541 ttcatattca gtaaagatat ttgcttagat aagattctat tattgtatgt aatatgttga
    12601 gatataaaag gttgaatgtc aatcatcata ttaaataact ctaagcattt acctgtatat
    12661 ttatatactc taaatgaagt tagaaggcca gttatatcaa aatcacccca attatatatt
    12721 gtaacatcat caaatttaca aaaccattta taaaaatcat ccataacata taaaagatta
    12781 ggagcattat tgatatctag ttggttcaat ttggttaaat ttttacattt atttgaaagt
    12841 attttattgt attttggctt aactaaagag taataattat ctattgtatt aaattcgtta
    12901 tcaactaaaa ctccaccaat agaaattaat tccgcaccat tatattcttt tggtttattt
    12961 ttattatcat ctagtatatt aaattcaaag tctataaata acttgtattt cataaatcta
    13021 aagcacatcc tcaggtaaat tatttattga gcctaagcat cataagaatc aatagatata
    13081 atgactctaa acatcaaagg ttcattacaa cttttattta tttcataatt taattcaaat
    13141 aaatc
//
LOCUS       DAKBNN010000108        12915 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000108 DAKBNN010000000
VERSION     DAKBNN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.109"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            290..958
                     /locus_tag="OUB02_002479"
     CDS             290..958
                     /locus_tag="OUB02_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886228.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            960..1445
                     /locus_tag="OUB02_002480"
     CDS             960..1445
                     /locus_tag="OUB02_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCT8886229.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1506..2627
                     /locus_tag="OUB02_002481"
     CDS             1506..2627
                     /locus_tag="OUB02_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCT8886230.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2908..4098
                     /locus_tag="OUB02_002482"
     CDS             2908..4098
                     /locus_tag="OUB02_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8886231.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4232..4487
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4546..5547
                     /locus_tag="OUB02_002483"
     CDS             4546..5547
                     /locus_tag="OUB02_002483"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCT8886232.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5651..6040
                     /locus_tag="OUB02_002484"
     CDS             5651..6040
                     /locus_tag="OUB02_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HCT8886233.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVITKKDKHSNIIENVEFSIKDEEE"
     gene            6201..7409
                     /locus_tag="OUB02_002485"
     CDS             6201..7409
                     /locus_tag="OUB02_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HCT8886234.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7495..8571)
                     /locus_tag="OUB02_002486"
     CDS             complement(7495..8571)
                     /locus_tag="OUB02_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCT8886235.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8800..9327
                     /gene="hpt"
                     /locus_tag="OUB02_002487"
     CDS             8800..9327
                     /gene="hpt"
                     /locus_tag="OUB02_002487"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCT8886236.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9416..10348
                     /locus_tag="OUB02_002488"
     CDS             9416..10348
                     /locus_tag="OUB02_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCT8886237.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIAAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNMSD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10410..11636
                     /locus_tag="OUB02_002489"
     CDS             10410..11636
                     /locus_tag="OUB02_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCT8886238.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11720..11941
                     /locus_tag="OUB02_002490"
     CDS             11720..11941
                     /locus_tag="OUB02_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HCT8886239.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12130..12300
                     /locus_tag="OUB02_002491"
     CDS             12130..12300
                     /locus_tag="OUB02_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886240.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12393..12854)
                     /locus_tag="OUB02_002492"
     CDS             complement(12393..12854)
                     /locus_tag="OUB02_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886241.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
BASE COUNT         4997 a         1348 c         2274 g         4296 t
ORIGIN      
        1 taaataatag gtttttttag gattttatcc tataaaaacc tattttaaaa ttatttttgc
       61 aagataagga aaatagcttt ttatattgta atgtatattt taaataatta ataaaaaaat
      121 attatatttt taaacttgaa aagtaagtaa ttataagcta tttaatcact ggaataaatt
      181 tacaataaaa aaataaatac aaaggcttta tcattaaggt attggcataa gttttgctta
      241 ttaaacaagt atactaataa aattcattta aacaggaggg agaatttata tgagtaaatt
      301 agaatcaaca acagaaaaag ttgaggtaaa atggtatggg tatgtagcat taatattggg
      361 agcattattt ttttcaggaa tatttaaaga tgcaccagga ccattaaaag tattggattt
      421 taacaatgta ttaggttctt ttggaacatt gggaacaatt aatgatggtg taggtacatt
      481 agctgcaaac tttagaggtg atggaggtac tggccctcga gatggatggc tatatgcatt
      541 gacattaata ccatcagtta tgtttgcact tggagtagtt agagttatag accacttaga
      601 tggaatgaag gcagctcaaa agcttcttag tccactatta aagccattat tagggttacc
      661 tggatttgca ggactaactt tgatagctag tttacagtct acagatgcag ctgcatctat
      721 gactaaagaa cttaaagatg atgggtatat agatgaaaaa caaaaagctg tattttgtgc
      781 atttcaattt tcaggtgcgt ctgcaattac gaatttcttt gcttctggag cagcactatt
      841 tccatttata ggagatgtac caatatttat accacttgct ttaatattaa ttatgaagtt
      901 tgttggggcg aacttactta gattatattt aaataaattt gacaaggggg aagcttaaca
      961 tgagtgaggt agcaaaaaaa gaagtaaaag ttaaaaaagg aaatgtgata gatgcgttta
     1021 ttggaggagc tagaaatgga ttccaaataa gtacaaattc aatggctcca aatgtcatct
     1081 tcggttttgc tataatttct gtgttcaatt taacaggtct tttggatgta atagggacaa
     1141 tatttacacc tgtaatgagt atctttggac ttccaggtgt tgcagcaaca gctataatga
     1201 caatatttat atcaatggga ggagcatctg gagtaatcgc aggtttattt acagcaggtc
     1261 aaataagtca agcacatgtt gcaattctat tgccaatgtt attgctttca ggagcgcaga
     1321 tacaatttat ggggagactt ttaggaacag ctgagttaaa aactaaatat tatccacata
     1381 tgtttgttat agcaacttta aatggttgta ttgcaatgct tataatgaat tatttcgtag
     1441 tataggtaat tataataaag atttaatata aatggaaagt ttaaatttta aggaggagct
     1501 agattatgga aactaacaaa gactatattg agtatgtaca taaagtgtat gattatctac
     1561 acactatacc agaaaaagga tttgaagaat ataagacatc agagtttgtt ttaaaagagc
     1621 ttgaaaaaat ggggtttgaa aatattaata aaggaattgc aggaactggt attatagtaa
     1681 ctatagattc taaaaaagaa ggaccagtat ttgctataag agcagatatg gatgcattgg
     1741 aatttatcat agatggagaa aagaaaatga tacatgcttg tggtcatgat ggtcatactt
     1801 ctatgctttt aacagctgca aagttaataa aagagagaaa tttagtaaaa agaggtaagt
     1861 taaaaatact ttttcaacca ggtgaagaaa aattatatgg agcattaaga gtaattgatt
     1921 ctggtttgtt ggatgatgta gaagaaatgg tagggatgca tttaagacct ggacaagatt
     1981 gtaggttggg aaaggcgata cctgcaatgt accattgtgc tagctgtata atggaattta
     2041 cgattaaagg tatcacttgt catggttcta aaccacattt aggagtaaat gcagtagatg
     2101 ctgtctgttt agcagtgaat gcaataaatg caataagagt aaatccagct ataccaaatt
     2161 ctataaaagt aacaaatata tcagttaagg gagaaactta taataatata ccagatgaag
     2221 catttatggc tttagatata agggcagaaa gtaatgatat aatagaagaa ttaaaagaaa
     2281 aagcaataca tgctgtaaaa actgctgtaa gttctataaa tgcagaagct gtactaattt
     2341 ccaaagatgg agtaccagca gcagaatttg atgaagaact tgtaaacttg gctgaagaaa
     2401 ctataaaaga tgtattggga gctgatgcat ctataggtat atttaaaaat tctggtggag
     2461 aagatttcca ttattataca caaaaactaa aatgtaaaac tacatatatt ggattaggag
     2521 ctgatgctac accaggattt cataatccaa atgtaacatt tgatacgaaa gctttagaat
     2581 atggtgtaga tatatggtgt agattggttg aaaagagatt gggataacat ccaaactaat
     2641 aattctacat ttaaaattat aattatagaa gttatagaaa aagattgctt atgttaatta
     2701 gaaaataatt aatatgagca atcttatttt gatataatga aactgatttt ggaatattga
     2761 aaaaatatga tgggaaagta attatatttt aaatctattg gacaaaaaag tgatattatt
     2821 ataatattaa gaattgtaaa ataataccaa agtgcatcta gtaatagact taacaattat
     2881 aaaaatttta ttcttaggag ggaaaaaatg agtaagtata attttgacag agtaatagat
     2941 agagtaggaa cagattgtgt aaaatgggat tttagaacta attgttcaac aaaagcacaa
     3001 aaagatggtt tgcctttttg gattgctgat atggattttg aatgtgcaga accaataata
     3061 gaagcattac gtaaaaaagt tgaacataaa atatttggat atagttcaaa tgatagtgat
     3121 aaatacttta atgcagtttg tgattggtat aaaaggagat ttaactggga aataaataga
     3181 aaagatatta tttttagtcc tggtgttgta cctgctgttg ctattttagt aagaatatta
     3241 actaattcta atgaaggtgt aatagtccaa aaaccagtgt attacccatt tgaagctaag
     3301 ataaagagca ataataggga tgttgtaaac aatcctctga tatatgagaa tggtacttat
     3361 aggatggatt atgatgattt agaagaaaaa gctaagtgta gcaacaataa agtactgata
     3421 ctttgtagtc ctcacaaccc tgttggaaga gtttggagag aagatgaatt aaaaaaggtt
     3481 gtagaaatat gtaaaaaata tgacctatgg ataatagctg atgagataca ttctgattta
     3541 attagaaaag gatttaaaca tactccttta cagtcattat gcccagagta caaggataaa
     3601 atagttacat gtatagctcc aagtaaaaca tttaatctgg caggaatgca gttgtctaat
     3661 ataatagtga ataatgatga actaaaaaag aaatatcaag aagaggttac agctgttgga
     3721 gttgcaactt caccaaatcc ctttgctatt gtagctacaa tagcagcata taatgaaagt
     3781 gaagactggc ttaatgaatt aaatgattac ttagataata atatacaatt tattgatgaa
     3841 tacttaaaag aaaacctacc aaaagtaaaa cttgtttatc cagaaggaac atatctagca
     3901 tggttagatt ttactgctta tggattaaat gaagtagaac ttgaggattt aatgtttaaa
     3961 aaagctaatg tattatttga tgaaggttat atatttggaa aagaaggaat aggatttgaa
     4021 agaataaatg tagcttgtcc acagtcttta ttaaaagaat gtatggacag attaaagact
     4081 atatttgaaa atttataaag tataggtaaa aatttataaa gtgtttaaaa acaaaaaaat
     4141 aataaacttg acaaatttta actaatcact tatcatacta ttatgaataa atagaaaaca
     4201 taagagagct aaattttatt gaattaaact cttaaagact ttgaaaggaa gagtagttat
     4261 agaatgtaat taaagcgagt tggtgatggt gtgagaccaa tatgaagtct ataatgaaga
     4321 acatcctgga gttgctaacc gaaattttaa aagagtaggc ttagtcggaa ccaaaccgtt
     4381 atcatattgg ggtgtatcga gtaatatctg tacattgtga gctgggtttg aatttatttc
     4441 aaactaatca gggtggtacc gcggaaaagc aacctttcgt cccttttata tagggatgaa
     4501 gggttttttt aatggaaaaa tatataaata tagggagtga atgatatgag cacaataata
     4561 ccaaaggatt ataaaagtag tttgaacgta attgatacac aaatagctat aaaaaaatta
     4621 aaagattttt ttgaaatgag actaagtaat gaattaaatc ttataagagt ttcttctccg
     4681 ttatttgtat tgccagaaac aggtgcaaat gataatttaa atggagaaaa ggctgtaagc
     4741 tttgatatac catttatgaa taaaaatgca gaaattgttc aatcacttgc aaaatggaaa
     4801 agattagccc ttaaaaaata tgggtttgaa gttggtagtg gtttatatac agatatgaat
     4861 gcaataagaa aaaatgaaga attagacaat atacattctc tttatgtaga ccaatgggat
     4921 tgggaattgg ttatagataa aagtagtaga aatgaaaaga ctctaaaaga tgttgtaaaa
     4981 agattgtatg gagtatttaa agatactgaa atttttgtat gtagtatgta tgaaggtata
     5041 aaagaaatat taccagaaga aataactttt ataacatcac aagagttaga agatatgtat
     5101 ccagaattat caccaaagga aagagaagat aaaattgtaa aagagaagaa agcagtattt
     5161 ataactcaaa taggaaaaac attaatatca ggagaaaaac atgatggaag aagtcctgat
     5221 tatgatgatt gggaattgaa tggggatata ttattttaca atcctgtttt agattcagca
     5281 ttagaattat cttcaatggg aattagagtt gatgaagaat ctcttgatag acaattgaaa
     5341 cttgctggat gtgaagagag aaaagagttt gactatcata agatgctttt aaatggcgaa
     5401 cttccgtata caattggtgg aggaattggt cagtcaagaa tatgtatgta tttcttacaa
     5461 aaagctcata taggagaagt acaagttgga gtatggccac aggatatgat tcaaaattgt
     5521 agtagtgcag gaattgaatt attataattt aaatataaac taattgataa aatttatatc
     5581 taatatgttc atcaaattaa ttatatcggt tataatgtag aataacctat aattataaga
     5641 ggtgataaat atggagtttt taaaaataat cattaatata gttttagata ttttaaaaaa
     5701 aattttggtc agatttaaaa atgctaaatt tggtttggtt tttgtatttg atttacttaa
     5761 actacctgat tttatgacag ataaaagaat caatatagtt gataaaataa aggtaatttc
     5821 tgtattaatt tttactatat cctactttgt atctggggtt gatataattc cagagatgat
     5881 tgcaggggca tttggtttta tagatgatgc tatagtttta atttggagta taggtatagt
     5941 gaatgaagaa attaataagt atagagttat cacaaaaaaa gataaacatt caaatataat
     6001 tgaaaatgta gaattttcta taaaagatga agaagaataa caatttatat atgttattct
     6061 tttttttatt aacaaaaaat ggttaaattg ttgacggaga ggtaaaactc tattatcata
     6121 ataagtggat atccaatatt taactaagta ttcaatgtgc aagaatgctt aatttaaaaa
     6181 agttatggag ggtaaatatt atgaagaaaa aaataggttt agtgcccaaa ctaatcatcg
     6241 gtatcatagt cggtatttta atcggaagtt atgcaccaga aataatagta caaatattag
     6301 taacagttag tactttattc tcagcattcc taaaatttgt tataccattt ataattatag
     6361 gttttgtaac agcaggtata gctgatttag caacaggtgc aggaaagtta ttggggataa
     6421 ctacgggaat agcatatggt tctaccttaa tagcaggatt attatcattt gcagtatcta
     6481 cattaatctt cccaaatttt attgatgcaa gtgttgcaag tcaaattgga gacccagagg
     6541 caggaatgtt agctccaata ttcacaattc cattagagcc tatggtagat gtaacagctg
     6601 caatcgtatt tgcatttgta atgggacttg gaatatcagc tttaagaaat catggaaaag
     6661 gagagacttt atttaatttc ttccaagaat ttcaaggaat cgtaacgaaa acgttgtcaa
     6721 cagtgattat acctttatta cctttataca tagcaggaac ttttgcaaat ataactatag
     6781 caggggaagt atggacaata ttaggggtat tctggaaagt atacttagta gtaatacctt
     6841 tacattttgt atatatggca tgtcaattca cagcagcagg tgtattttca ggaaaaaatc
     6901 cagtaagaat gttaaaaaat caagtacctg gatatttaac agctatagga actcaatctt
     6961 cagcagcaac tataccagtt aatgttggtt gtgcagaaaa aaatggagta agtaagcaaa
     7021 taagagaatt tgttgtacct ctttgtgcaa caatacattt atcaggtagt atgatatcaa
     7081 taacaggatt tacaactgca gttttaatga tgaatggtat gccacatgga ataggtgtat
     7141 tattccctta tattgcaatg ttaggtatag caatggttgc agcaccagga gcaccaggtg
     7201 gggctataat gagtgcactt cctttcctac caatggttgg aataccatca gatggtgggc
     7261 ttgcaagttt aatgatagct ttatacttaa ctcaagacag ttttggtaca gcatgtaatg
     7321 tatcaggaga taatgcaata gcagtagtag tagataaaat aaatgatgga atgagtaaaa
     7381 aaggaaacaa agaagagcaa agtgcttagt tttttaataa tcaaaaatga gttatctcaa
     7441 atagagatag ctcatttttt atggtataaa aaattataaa ataaagtgat ttttttattt
     7501 aaaaattgct ttaagagatg atattaataa tttattttca ttaggtttta atatacatac
     7561 tctaaagaaa tactcatcta gaccttcaaa tgaacaacaa tctcttatta ttattttttg
     7621 tgggagcagt tgctctcgaa gagatttagc agtcaattcc tttgtcttga ttttacacaa
     7681 tatgaaatta cctttagtat tgtatatatc taagcttttt atattcttaa gctctttaag
     7741 caggtaatct ctttcagtgt tcattaagga aatagtattt gatataaaat ctaaatcact
     7801 aaacatgatt tcacccattt tagaagctat tatgtttata ttccataaat ctagattttt
     7861 atttatttca tttttgacat tagtgttaga tataagacca tatccaagcc ttattccagg
     7921 cgttgaaaaa aattttgatg tacctcttat aacgaataaa ttagaataat catcaactaa
     7981 ttgagtacaa gagtatgtat ctgtatcagt aaactctaca taagtctcat caatcattaa
     8041 aaatgagtct gtatttttta aaatttctct cacttctact ttagtaaatg caaatccagt
     8101 tggattacta ggattacaga ttactaccaa atcataatct ttagcattga tagtttttat
     8161 aagattttct aaatttatat gaaaattatc ttcttctttt gcaaagtatt tttctataga
     8221 acaattaatt ttagataact ctttttcata ctctgaatag gctggagaga gtaaaagagc
     8281 ttgtttagga ttaattgtat gtataaatga tgaaattagt tctgtagctc cactgccaag
     8341 tactatatta tctatagaac atttgcagta attggatata gaagttttta agtctatgta
     8401 atcagggtca ggatacatag ataccatatc tatattgttt acaatgtatt gtttggctaa
     8461 acttgatgta ccaaaaggat ttatatttga gctaaaatcc ataaattctt cctttgaaaa
     8521 gccatactta ctagataaac tatataaatt agctccatgg tttgtgctca taaaagtacc
     8581 tccaaaaata aaattatata atcgataaaa ataactgatt aatatatgta catatagatt
     8641 ataatacaaa ggaatcgtta ttccaaattt ttaatgtaaa aagcaaaaaa gtatggataa
     8701 gctgttaatt taggtttaaa aatgatgaaa aagattttaa ttgatatttt ggtatgctat
     8761 aatatataag ttaatagaag gaaagcggga ggatacaaaa tgtacaaagt tacaggaaaa
     8821 atgttgacag aagaacaaat aaaagaaaaa gtttatgaat taggaaaaaa gatagaagaa
     8881 gactttaaag gcgaagattt actagtggta ggtatactta aaggagctag tgtatttgtt
     8941 tcagatttaa taagatgtat agatttggat gtaaatatag attttatgag tgttacaagt
     9001 tatggaaact caacagagtc ttctggaaca gttaaaatat taaaagattt agatgtagat
     9061 attgaaggaa agaatgtgtt gattgtagag gatataatag attctggttt aacattaagt
     9121 aatctagttg cagctttaaa aactagaaat ccaaaatctt taaaactatg tactctactt
     9181 gacaaacctc agagaagaaa agctaatata cctgtagact atgttggatt tgttatagaa
     9241 gacaaattta ttgttggtta tggaatagac tatgctgaaa agtatagaaa tttaccttat
     9301 ataggtatag ttgaggatgt agaatagatt taaatgtgaa tcaaaagcac taatttctaa
     9361 gggagttagt gctttttagt taattatata aaattttagt aaaggagaca tctatatgga
     9421 tattcagtta aatatattat tatttttcca aaatttaaga aatccaattt tgaactttat
     9481 atttcttgtc tttacaataa gcacagaagc accgttatta atattaatta caacaattat
     9541 atactggtgt ataaataaaa aatgtggaca gaaaatactg tttgctataa ttggaaattt
     9601 tgttgtaaat ttaggcatca aagagtttgt aaaagctccc agaccaatag gaataaaagg
     9661 tcttgaatca ttgagagttt ctactgctgg tggttattct tttccaagtg ctcatactca
     9721 aactgcaaca acattttggg ttagtattat gactatattt aaaaagagat atcttcattt
     9781 aatagctgca gttatggttt taggagtagg attgtctagg ctgtatttag ctgttcattg
     9841 gccaatagat gtgatagcgg gttggatatt aggaatattt tttacagttg tatttgtaaa
     9901 aatatttgac tatattgatg ataataaaaa ttatatttta cttgtagttt tacttatacc
     9961 atttataata gtggctatat ttctaaatag ccctgaatat tttgaaaaat ttggtataat
    10021 agtaggattt gtatttggat atatggttga ggatagattt gttaaattta atacagataa
    10081 taataacaaa aaaataaatt ttagtaataa aaataagggg agtaaaaata taattttttc
    10141 aagaatatgt aggtttttgg ttgggatagt aacaatagga atgttatata ttggcattaa
    10201 gttgtttatt tcaatgttaa tagtcacttt aaatatgagt gactctacta tgagtataat
    10261 ttttatgaat tttattaaga atacagtagt tgttttttat ggaatagcag gagctccagc
    10321 attatttaaa cttttaaaat taaattgatt ttaaggtata gagtcaatag tttaaattaa
    10381 aaaaattaag ggtagaaaag agtgattatt tgaagaaggt agtaataata ggtggtggac
    10441 cagcagggat gatagccgca tcaactgcat gtgagaaagg ctatgatgta acattaattg
    10501 agaaaaatca taaattagga aaaaaacttg caataacagg aaaaggtaga tgtaatatta
    10561 ctaacgcttg tgaaatagag gaattaatag aaaatgtacc tacaaatgga aaatttcttt
    10621 atagtgcttt ttatactttt acaaatgatg atgtaataag tatgtttaat aatcttggag
    10681 taaagactaa aacagaaaga ggaaaaaggg tttttccaga gagtgataaa gcatttgata
    10741 ttgtaaatgc tcttgaaaga caacttaaaa gtaagaaagt aaatatatta ttaaattcga
    10801 aagtagaaaa aataatatct aaaaataata aaattgaaaa agtaatttta aacgataaaa
    10861 aagaaattaa gtgtgattca gttgtagtag caactggtgg attaagttat ccacttactg
    10921 gttctactgg tgatggatat aaatttgcaa tttcacaggg gcacacaatt atagatacaa
    10981 aaccatcttt aattggtata gaagtacaag aaagttttac aaaagattta gaaaaattat
    11041 cattgagaaa tgttgaaata agggtgttta atagcaaaca aaagaaagtt tatagtgatt
    11101 ttggagaact tgaatttact agatttggtt tagatggacc tataatcaag tctgcaagtt
    11161 gtagaatgaa agatacaagg aaagaaaatt acactatatt attagattta aaacctgcat
    11221 tagatgagga aaaattggat aaaagagttc aaaaagattt tcagaagtat acaaataaaa
    11281 aatttgaaaa agcattagat gatttacttc caaaaaaact aatacctata ataataaact
    11341 taagtgaaat taatccaaat acggttgttc accaaatttc tagagagcag agaaaaaact
    11401 tagtccattt attaaaaaat ttaaagttta cagtaaagag atacagacct attgaagaag
    11461 ctataataac ttctggtggt gtaaaagtaa acgaaataaa ttcaagtaca atggaatcaa
    11521 aattagtaga aggtttattt tttgctggag aagtaataga tatagatgca tatacaggtg
    11581 gattcaattt gcaaatagct ttttctactg gatatttggc aggttttaat tgctaaaaat
    11641 tacctgaatt aaaaatgttt aaaatcacat aatattatca gcaacacaaa ataaattaaa
    11701 cagaaggaag gtaatcaaca tggcaagtaa caataacaac aacagaacag tagtaccagg
    11761 agcaaaagag gctttaaacc aaatgaaatt agaaatagct aatgaacttg gtatgagtaa
    11821 ttatcaacaa gttgataaag gaaaccttac tgcaagagaa aatggttatg taggtggata
    11881 catgactaaa aaattagttg aaatggctga acaacaaatg gctggaaaaa gcaacagata
    11941 gttataaata attatttata actattggct attagtttag ctagagctat ctcaaatata
    12001 gaaagtaatt tctaaattga gatagctttt ttttattaaa ttattaaata gatatatttt
    12061 tatgtaataa attttatgta tttggataaa ataatacaaa tgaatatgtt acataaggag
    12121 tgatgtaaaa tgagtagtgt aaattttgag gatttaaaaa acaaatttat taatagtgac
    12181 ttagatgaaa aaataagaat atatacaaca acagaaggtt tatcagtaga acaatttaaa
    12241 gagctactaa aatattatcc aatacaacat ttatctaaac tagaaaaagc acttggatag
    12301 cagtatttaa tccaaaatta aaaaggcaat ttatttaata ttaaaaatat tttttattaa
    12361 ataaattgcc tttttataat aggtttaaat tattatgctt ttgatatata attatagatt
    12421 ctctcatttc caagttcaat agacccagta ttaataagag taactattcc ttctatatct
    12481 ctagcagtta agtatttata tggaattaat aaattataac cagtattact tagtaagttt
    12541 tttaattggg ttatttgaga gatattgaaa cattttgcag ttttagtaga tttttttctt
    12601 aagttagata aattttcatt ttgaatattt attaaagttt ctatatagtt tctataagat
    12661 atcttaaagt ttgtctcatt gactattttt tcattaagta tactactgat aaaatcttga
    12721 gacaatatat tatctgcaaa tttatctgct gttaattttc cagatgttat gcctttttga
    12781 tattttctaa gaattttttt agtatagcag tatattttat ttctcgtttc taaatcgagt
    12841 tgttctattt gcacaaaacc acttccttct aattataaaa atatttatta aaaatctata
    12901 cttaactaat aaagc
//
LOCUS       DAKBNN010000109        12812 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000109 DAKBNN010000000
VERSION     DAKBNN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.110"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..242
                     /locus_tag="OUB02_002493"
     CDS             <1..242
                     /locus_tag="OUB02_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886242.1"
                     /translation="DKTREKFSKIKLNEIKKIYETIGINSITKISIDKNKLEMEGVCS
                     DLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            257..841
                     /locus_tag="OUB02_002494"
     CDS             257..841
                     /locus_tag="OUB02_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886243.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1094..2131
                     /locus_tag="OUB02_002495"
     CDS             1094..2131
                     /locus_tag="OUB02_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HCT8886244.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2212..2577
                     /locus_tag="OUB02_002496"
     CDS             2212..2577
                     /locus_tag="OUB02_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886245.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2582..2899
                     /locus_tag="OUB02_002497"
     CDS             2582..2899
                     /locus_tag="OUB02_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886246.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            2947..4551
                     /locus_tag="OUB02_002498"
     CDS             2947..4551
                     /locus_tag="OUB02_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886247.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4675..6303
                     /locus_tag="OUB02_002499"
     CDS             4675..6303
                     /locus_tag="OUB02_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCT8886248.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6568..7917
                     /locus_tag="OUB02_002500"
     CDS             6568..7917
                     /locus_tag="OUB02_002500"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCT8886249.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8073..9152
                     /locus_tag="OUB02_002501"
     CDS             8073..9152
                     /locus_tag="OUB02_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCT8886250.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9497..10405
                     /locus_tag="OUB02_002502"
     CDS             9497..10405
                     /locus_tag="OUB02_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCT8886251.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10410..12779
                     /locus_tag="OUB02_002503"
     CDS             10410..12779
                     /locus_tag="OUB02_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCT8886252.1"
                     /translation="MSRGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
BASE COUNT         5266 a         1326 c         2371 g         3849 t
ORIGIN      
        1 cagacaaaac tagagagaaa ttttctaaaa taaaacttaa tgaaataaaa aaaatatatg
       61 aaacaattgg aataaatagt attactaaaa tatcaataga taaaaataag ctagagatgg
      121 aaggtgtgtg tagtgattta gatgttttaa acaatttaag gaaaataaag tacataaaat
      181 attattacat agacagtatt gtaagagatg gagaatatta tagatttaga ataaaaagtt
      241 agatgggtga ttatttttga gattaaataa tagagaaaaa aatctactta aaatattgtt
      301 aatattagtt attatattgg caagtgatat agcagtaagt tatttggaag aaaacctaac
      361 tagtttgaag aatgaaaagc aaaatataat aaataaaata aataatcaag ataattatca
      421 aataaaggat aataagcata tagatgataa atatatagaa agtgttgaat caaataaaaa
      481 tgaagaatta aaaaataatt ttaataactc atttttagat aaaaaaacaa ataaagatat
      541 agtgttatta gtagaaaatg aactgagaga tattgtgaat gtagaatata ttaatcaaga
      601 aagtagttta gataatcaca ataaaaaaca agttaattta gaaataaaag ttaatggaga
      661 acttgcaaat gtgttgaaac ttgaaggtgt aatgaagaag ttaagactaa ataataaact
      721 tgaaaagata gaaattaata aattaaaagt caataatttt gatgttcaaa atattccaaa
      781 tgaatataaa caagaaaaag acaatattgt tgaatgtaaa atgacagtta gagtcatata
      841 acaaaaaaga atagataaag taaaaaacta ttatcaatta ataaaatttt atagaggaat
      901 tagttttacg ataattcatt tctacaagag ggattgttga tgattttatc gaaatcctaa
      961 ttaaagttaa tatattgtac caaaatataa gtgaatttaa tagaatattt acttatacta
     1021 tagattgatt aataaaaaaa tggtataata acaaattgta ctttaattta ataataaggg
     1081 agtggaagtc atattgttag ggagaaatga gctatgccct tgtggaagtg gcaaaaaata
     1141 caagaaatgt tgtttaaata aagatattgt ttcagaaaga gcatgcagaa aaactgaact
     1201 ttcacaaaaa caatatacag atttatattc aaagttatat gattattcaa gacaggataa
     1261 gtttaaagaa gaatgtgaaa aagctaaaga aatgttttat ataatgcaaa atgatagtgt
     1321 aaatgaaaaa tttgaaagat tttttaatac ttatttaata caagaccaca taatggaaaa
     1381 taaaaaggtt atgacagttg gttttcttga agaaaatggt gcaaacctaa gtcaatcaga
     1441 agtatcaata ttaaaaagta tgtttgaatc atacgtaagt atttatgagg taaaagaagt
     1501 ttctgctgga aaaatagtat taaaggactg tttaagtgga aatgaacttt gtacagaaga
     1561 tgtaaaactt ctgagaagtt ttaaaatcgg aagttgtatg atagctagaa tagttgatat
     1621 tgaaggaact aacatattga tagatataac tataagtata tcaaatgagg ttaaagatat
     1681 aattttaaag gatattatga atctattcaa tcaatatcaa gatgtataca aagatatgaa
     1741 gtcgttctta atttatcata cacatatact atacaagtat atacaacagc ttttagaccc
     1801 aagtatagct gaatatttaa agagagaaag agataaaaac gatacaaaag ttaattcaga
     1861 aaatgaatta gctgaagaag aatgtaaagt tctagatact cttaaagaaa atgtagaaaa
     1921 agaaaatata gatgcatgta tagagttttg gaatgaatat aagaaaaatc atgaaaatat
     1981 aaaaggagct gaaaatggtt gggcagctgc tgttgaatac tatgttaaaa aagaagcagg
     2041 acaacctata actcaagttc aaatatcaaa gaaatacgat ataagctcta gtacactagg
     2101 aaagagatac aaggatttaa aaatatctta attaattata aaaagcttta agtcatagat
     2161 aaagaatcta tagtataata tatgtataat gtttagaaag aggtattaat aatgagtctt
     2221 agtttaggaa atgcaattgc tgtatcaacg aactttataa aatctaaccc agctgtacct
     2281 gtgtacaacg gagaagaaat atatgcagtt atttatcata tagaaatagg atataataat
     2341 ggtgtatcac ctgataccaa acaatatata ataatagact ataaagagtt tgatttatct
     2401 atggataaaa aaaatgaact aataaatatt gctaaagagt actgtaaatc taaaggtatt
     2461 gtagaaaacg aaaaccttga aataaacctt ttagacaatg aagaagcaga gaacttctta
     2521 gaaaaggtgt ttaataatat gaaggttata agaggtctta taactaaaaa aggataaata
     2581 aatggactat ataggaatag aaaatataac accttatgaa aatacatatg aatttagtgt
     2641 atatgaatat gatgatgaaa tcaccttagg tagtgaaaag ttatatgtat gtgaattaag
     2701 ggttgtattg attaaagtta attctctgta tgttgaaaga ttgcataaat cagttgaagc
     2761 aatggtctta gtaaaaaact tgaaaaaaga tttagataaa acacttgttg taaacaaaat
     2821 aaagaatttt gtgctagatg agatttgggt agaaaatcta gtaaaagaga atatagaagt
     2881 tatatttgta gaaagctagg aataacccta gcttttttat attacaatct aagagaggaa
     2941 aacaatatgt taagagtatc taatgtaaaa cttggaattg ataaagatat atctttatta
     3001 aaagatgtta tacttaagaa gctaaaaata aaagaaaaaa acttagtcaa atatagtata
     3061 tataaagaat ctatagatgc tagaaaaaaa ggaaaaatgg aatttgttta ttctgtagat
     3121 gtagaagtta aagatgaaaa taaaatactt aactcaaaaa taaaagatgt aacaaaaata
     3181 aaggaaatca gatacgtaaa tgtacctgtg ggtgataaaa aattaaagaa taatcctttg
     3241 gtaattggta gtggaccagc tggcttgttt gcaggtctgc ttttagctca aatgggttat
     3301 gaaccaatca tacttgaaag aggtcttgat gtagacagta gaaccaaaga tataagtgat
     3361 ttttggacaa ctagaaaatt aaaaaataat tcaaacgttc aatttggaga aggtggagca
     3421 ggaacttttt cggatggtaa acttacaact agaataaaag atatacgatg tagaaaagta
     3481 ttagaagagt tagttcagtt tggagcacca gatgaaatat tatactcaca taagccacat
     3541 gttggaacag atatattaaa aaatgtagtt aaaaatataa gaaatgaaat aataagttta
     3601 ggtggtcaag ttagatttaa ttcaaaagta acagatatag ttgtatcaaa tgattctata
     3661 caatctgtaa taataaatga tactgaaaaa atagatacag atacaattat attagctgtt
     3721 ggacatagtg caagagatac ttatgaaatg ttgtattcaa gaggtataaa aatagttcaa
     3781 aaaccttttg caataggagc tagagttgaa catccacaag atttaataaa taaagcacag
     3841 tacaaggatt tttataatca tcctaggtta ggagctgcag attatagact tatagagcat
     3901 gtttcaaatg gaagaacagc atatactttt tgtatgtgtc caggaggtag tgtcatagca
     3961 tctgcatcaa atgattttga aatagttaca aatggtatga gtgaacatgc aagagataaa
     4021 ataaatgcaa atagtgcatt tttagtaaat gtaacgccag aagattttaa gagtgataat
     4081 ccactagcag gtgttcattt ccagcagaag tatgaaaaat tagcttttga attaggtgga
     4141 aaaaattata atgcaccagt tcagcttgtt ggagatttta taaataataa gacttctttg
     4201 aatttaggaa gtgttgagcc atcatataga ccaggatata cacttacaaa tttaagtgaa
     4261 tgtttgccta gcttcgtaac agagactatg agagaagcgt ttatatctct tgataaaaaa
     4321 ttgaatgggt ttgcaatgta tgatgctgtt ttgacgggag tagaaactag gtcatcatca
     4381 ccaataagaa tagtaagaga tgaaaatact ttagaatcag tgagtattaa gaatctatat
     4441 ccatgtggtg aaggtgctgg atatgcaggt ggtatagtta cagcagcagt tgatggtata
     4501 aaatgtgcag aaaaaataat acaaaaatac accaataata caaaaattta acataaactt
     4561 aatgattttt taatatttaa catgaattta acattatcac tcctataatt tgataaaata
     4621 caataggtta taaaattttt agatacagta atctctttac aaggaggaaa taatttgaac
     4681 attataataa gtctaatggg tggattaggt ctgttcttat atggtatgaa ccttatggga
     4741 gaagggcttc aaaaatctgc tgggacaaag ctaaaaaaga taataaaact acttactagt
     4801 aacctattta tgggagtttt ggtaggtaca ggagttacag cagtaataca aagttcaagt
     4861 gctactactg taatggttgt tggtttcgta aatgcaggaa taatgacttt aaaacaggca
     4921 ataggagtta taatgggtgc taatataggg actactgtta cagcccaatt ggtatctttc
     4981 gacttaactg gtatggcccc agtagcatta ggtgtaggta ttatacttta tttatttgga
     5041 aataagccta gaataaaaaa tatagccgaa atacttatag gttttggtat attatttaca
     5101 ggtatggatt ttatgaagat ggcagtggag ccactaagag actatcaagg atttactgac
     5161 ttacttgtta catttggtag atatccacta cttggattat tattgggatt tgggattaca
     5221 gcaataatac aaagttcaag tgcatctatg ggtatgttgg ttgcactggc agcagaaggt
     5281 ctagtgccac tatcagcagc actgccaatt ctttatggcc aaaatattgg tacatgtgta
     5341 acttcactgt tatcaagtat tggtgctaac aaaaatgcac gaagagcagc tatgatgcat
     5401 ctaatcttca atgttttagg aacagtgata ttcttaatat tcttgaataa accagtagta
     5461 agcatggtta caagttggga cccaagcaat gttgcaagac aaatagctaa tacacataca
     5521 ttgtttaata taatatctgt attgatactt ttaccattta caaatcttat tataaaacta
     5581 gctataaagc ttgttcctga tagagcaggt gatatagatg aagatgaaac taaaactatt
     5641 aaatatatag atgatagaat gatagaaact ccttctatag ctcttgcaaa tacgataaaa
     5701 gaagcacttc gtatgggtga gaaggcgaag gaaagcctaa atgcatctat ggaagcttta
     5761 gtagaacatt caactgaaaa aatagataag acatataggc gagagagatt aatcaatgat
     5821 ttacagaaag ctattttaaa ttatttactt aaactttcaa aagcaccttt agatgatgat
     5881 tcaagagaag ttgtggatac tttgtttaat acagtcaatg atatagaaag aattggtgac
     5941 cacgctaaaa atatagctga attatcgcaa gttgcaatag attcaaatat ttctttctca
     6001 gaagaaggac aatctgaact tgatgttatg tataatagag ttgtttcagc atatacttat
     6061 gcattagaat caatgagaac tgataatgtt gatttagctt gtaaggttat aaaaattgaa
     6121 gaacaagttg atataatgga gaaatcttgt agagctaatc acatgtatag attgaacaat
     6181 aatctatgta gtatagaaaa tggagttatt tatttagacg taataagtaa tttagagaga
     6241 atatctgacc atgctgtcaa tatagcacaa caagttatag caaagagatt aggaaatgat
     6301 taaaaataat taatttaaaa taatataaaa tttaaaatag aggataaata aaagagaaac
     6361 tttaaatgat acgtttagag tttctttttt tgtaaattta tttgactaaa atttttaaat
     6421 ttatacatag ttatagcatt tagaagaatc gatttcctga taataaatga tgagatatgt
     6481 atgattttgt ggcaaacttt taaaaaaaac acctatattt tgtgttataa tctctttaaa
     6541 acgaatatgc tgaaaagagg gatttaaatg ggaaaaataa attttgatta tagtaaagct
     6601 acagagtttt tttgtcaaaa cgaaatagat gtcatgcaac catatgtaga tgttgcgcac
     6661 gacatgttac ataataaaac tggtttagga aatacttttt taggatggat agaccttcct
     6721 aaaaattatg ataaagaaga gtttgacaga ataaaaaaat ctgctgataa gataaaatca
     6781 gactcagatg ttcttttggt aataggtata ggaggctctt atttaggttc gagagctgct
     6841 atagatatgg taagtcattc ttttaggaat ggacttaaaa aagagcaaag aaaggctcca
     6901 gaagtatatt ttgtagggca taatataagc tcaacatata ttatggattt attagatata
     6961 attgaaggaa aggatatttc tgtaaatgta atatctaaat caggaactac tacagaacca
     7021 gctctggctt ttagaatatt taaagattac ttagagaaaa agtatggtaa ggaagaagca
     7081 agaaagagaa tttatgcaac aacagatgca agtaaaggtg cacttagaca gttagctaca
     7141 gaagaaggat atgagacttt tgtaatacca gatgatgtag gtggtcgttt ttctgtattg
     7201 acagcagttg ggttacttcc tattgctgct gctggacttg atattgatgc tatgatgaaa
     7261 ggcgcaaatg atgctagaga agcatttcaa aatcctgact taaaaagtaa tgatagttat
     7321 agatatgctg ttgctagaac aattttacat agaaaaggaa aagatgtaga gttattagtg
     7381 aactatgaac ctcaactaca ttatgtaagt gagtggtgga aacagcttta tggagaaagt
     7441 gaaggaaaag aaaataaagg tttattccct gcttctgtag atttctcaac agatttacat
     7501 tctatgggtc aatacataca agatggaaaa aggcttttat ttgagacagt acttaatgtt
     7561 gaaaactgta agagaaatat aactataagt tcagaggaag tagacttaga tggtttaaat
     7621 tatttagcag gcaagactgt agattttgta aatcataaag cttttgaagg aactctattg
     7681 gctcatacag atggaaaagt acctaattta gtaataaata tacctcaact agatgagtat
     7741 aattttggat atttagttta tttctttgag aaggcttgtg gaataagtgg atatttattg
     7801 ggagttaatc catttgacca accaggagta gaagcttaca agaaaaatat gtttgcttta
     7861 ctaggcaaac caggttatga aaaagaaaag gaagagctag aaaaaagact taaataattt
     7921 taaatagtta aggtttaatt aagattaaaa taatataatg aaatcatcga aaaggagttt
     7981 actcaaaata gatgatttca ttttttttat gaaaaattgg gaaaaatata ttttgataat
     8041 ttatattata ttaagatagg gagtggtatg agatgtcaag aagaaagaaa gggataagtt
     8101 tagtaatatt agttgctata ataagttcaa tacttagtag ttttctaaca ataattttag
     8161 ttaaggacaa tttagtaagt aaatctacag gaagttctac gccgatagtt gtaaatgatg
     8221 atggtaagag tcaaaatatt tatcaagcag ttgcagaaaa agctacgcca tctgttgtag
     8281 gtattacaac aactagtgtt gatactagta atatgtttgc aataccaact gaaacacaag
     8341 gtgtaggaac tggaatcata gttgattcaa atggatatat acttactaat tctcatgtta
     8401 tatcagatgg tcaagctaca agtgtaaatg tattattcaa tgatggttca acaacttcag
     8461 gtaaagtagt atggtttgac caacaacttg atttagctat agttaaggtt gataaaactg
     8521 gactaacacc agcagaattt gctgatagtg acaaagtaaa agttggagat atatcaatag
     8581 caataggtaa tcctcttgga ttagattttc aaaaaacagt tactcaaggg ataataagtg
     8641 gattagatag aactatacaa acagaaaaaa ctaatatgac tggtttatta caaactgatg
     8701 caagtataaa tgcaggaaat agtggaggac cattgttaaa tcaaaaaggt caagtaattg
     8761 gtataaacac agcaaaagct tctcaagcag aaggattagg atttgcaata ccaatcaata
     8821 cagctaagag tatagttgaa gaagtaatca aaaatggaaa atatgaaaaa gtaactttag
     8881 gcataaaagg tacagatgta tcaaattatg aagcagcaac aggtacaaaa ttaagtactg
     8941 ataaaggtgt ttatgttgca gaagtaatat ctggttcatc agcagaaaaa gctggagtaa
     9001 aggtagggga tattataact aaagtaggag atacagatat aacaggtatg aatgatttaa
     9061 ataaaaagtt gtatactttc tcaaaaggtg ctagtacaaa aataacagta aatagaggcg
     9121 gaaaagcagt aacaataaat gtgaatttct aaatatgacc agaaagatag agactacatg
     9181 gtaaagtgta gtctctattt ttatttaatt tttatagtat atacatctaa attaattaaa
     9241 atgatgtatt gtttatagtt tattgctata ttttcttata aaaataaatt taataaatat
     9301 ttaaatttaa tttacaaaaa caatataaat atcataataa aataaaattt tttaatatta
     9361 ctaaaaatat atagacaaaa taataacaaa tatagtatta tgtaattgta gaaatgcttt
     9421 tcataggtat attctatgaa gatgaattgc ataaaaatga aaaaatggaa tggatattat
     9481 ttaggggtga aaaatcatga atccattagt tataaactta caaaaatgta gcatacatga
     9541 tggaccaggg ataagaagta cagtattttt taaagggtgt ccacttgaat gtgtttggtg
     9601 tcataatcca gagagtcaaa catatacaaa acaagttttg tataatgaag aaagatgttc
     9661 aaaatgtgaa gcatgtataa atatatgccc tcataaggca atttacaaag gtgaaacaaa
     9721 aatatgctta gaccaagata agtgtgagtt ttgtgaaact tgcttagact attgtgtaaa
     9781 caatgcaaga gagattgttg gtcaagagta ttctgtaaga gatttggtta aagagataga
     9841 gaaggataga attttctatg aagaatcagg tggtggagta actctatcag gtggagaagt
     9901 aatggctcaa gatatggact tcatttgtgg agtaataaac atgtgcaaat caaagggaat
     9961 ccatgtagca attgatacat gtggctatgc aaagtcagaa aattatgaaa gagttgctaa
    10021 atgtgcagat ttgtttttat atgatattaa gcttattgat gaagataagc acataaaatt
    10081 tacaggaaaa tcaaatgact taatactgaa aaatgttaag attttaagtg aattaggggc
    10141 taacataaat ataagaatac cattaattgt aggagtaaat gtagatgatg aaaatttaga
    10201 ggttaaaaaa atgatagagt ttttaaagcc tttgaatata caagcagtaa gtcttcttcc
    10261 atatcataat attggtaaac acaagtatga taaaatttat aaaaagtatg aaggagaaga
    10321 actacaaaga ccatcagaag aaaaattaga ggaaataaaa agactgtttg aagcaagtaa
    10381 tttcaatact aagattggag ggtaataaaa tgtcaagagg aacttttgag agaactaaaa
    10441 aattaagaga agaaagtatt aatgcagaac cacacatatc tattgaaaga gcagtactta
    10501 tgacagaagc ttataaaaaa tatgagggaa gtgtagagat tcctgtactt cgtgctttat
    10561 catttaagca ttatattgag aatagaactc ttagcataaa tgatggagaa ttaatagtag
    10621 gtgaaaaagg tgattctcct aatggagcac caacatatcc agaaatttgt tgtcatacaa
    10681 tggaagattt agaagtaatg cacaatagag atataataaa ttttagcgta tcagaagaag
    10741 ctagaaaaat acataaagaa gaaataattc ctttttggaa aaagagacaa acaagagata
    10801 agataataaa tgcaatgaca cctgagtggt tagcagcata tgaagcaggt atgtttacag
    10861 agtttatgga acaaagagct ccaggacata cagtttgtgg agatacaata tataaaaaag
    10921 gatttttaga tttaaagaaa gacatagaag caagattaaa agagctagat tttcttaatg
    10981 atttagatgc atataataaa aaagcagatt tagaagctat ggcaatagct tgtgatgcaa
    11041 tggttatatt aggtaaaaga tatgctgaga aagcaagaca aatggcagaa gaagaaacag
    11101 atgaagctaa gaaaaaagat ttattactaa ttgctgaaac ttgtgatgtt gtaccagctc
    11161 acaaaccaga aacttatcat caagctatac aaatgtattg gtttgttcat ataggggtta
    11221 ctactgaact taatatatgg gatgctttta ctccaggaag acttgaccaa catctaaatc
    11281 ctttctatga aagagatgta gaaaatggaa tactagatag agatagagca caagaattac
    11341 ttgaatgttt atgggttaaa tttaacaatc agccagcacc accaaaagtt ggtataacat
    11401 taaaagaaag tagcacatat acagattttg caaatataaa cactggtgga ataaatccag
    11461 atggtcaaga tggtgttaat gaagttagtt atataatact tgatgttatg gatgaaatga
    11521 aattgataca acctagttca aatgttcaaa taagtaaaaa gactcctcaa aaattcttaa
    11581 agagagcttg tgaaatatca agaaaaggat ggggtcaacc agcattttat aatactgaag
    11641 caatagttca agagcttatg gaagctggaa aaactataga agatgcaaga cttggcggaa
    11701 ccagtggttg tgttgaaact ggatgttttg gtaaagaagc atatgttcta actggatata
    11761 tgaacattcc aaaaatactt gaattaacat taaataatgg atatgaccca attagtaaaa
    11821 aacaaatagg tattgaaact ggagacccta gaaatttcca atcttatgag gaactatttg
    11881 aagcatttaa aaagcaatta cattatatga ttgacataaa aatagaagga aatgctgtga
    11941 tagaaaatat atgtgcaaaa cacatgcctt gtccacttat gtctacaata gttgatgatt
    12001 gtatagaaaa aggcaaggat taccaaagag gtggagcaag atacaacact agatatatac
    12061 aaggtgttgg tataggaact ataacagata gtttaacagc aataaaatat aatgtatttg
    12121 ataagaaaaa attcgatatg gatacattgc ttaaagcact tgatgctaat tttgaaggat
    12181 atgaagctat acttaattta gtttcaaata agactcctaa atatggaaat gatgatgatt
    12241 atgctgatga aataatgcaa gagatattca atgcatatta caatgaggta actggtagac
    12301 caactgtttg tggtggagaa tacagagtag atatgttacc aacaacttgt catatatact
    12361 ttggagaaat tatgggagca agtccaaatg gtagactttg tgcaaaacca gtttcagagg
    12421 gaatttcacc tgaaaaaggt ggagatacta atggacctac agcagttata aaatcttgtg
    12481 ctaagatgga ccacataaaa actggtggaa cattattaaa tcaaagattt gcaccatcag
    12541 tagttcaagg agaaaaaggg ttagataata tggctaatct agtaagagca tactttaata
    12601 tggatggtca tcatatacaa tttaatgtat ttgataaaaa tgtactgtta gaggctcaaa
    12661 agaatcctca ggattataaa gacttaatag ttagagttgc aggatatagt gaccatttca
    12721 ataatttaag tagaacacta caagatgaga taataggaag aactgagcaa acattctagg
    12781 tttgcttgta aataaaactt cttactatat aa
//
LOCUS       DAKBNN010000110        12800 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000110 DAKBNN010000000
VERSION     DAKBNN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.111"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            81..521
                     /locus_tag="OUB02_002504"
     CDS             81..521
                     /locus_tag="OUB02_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HCT8886253.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(706..894)
                     /locus_tag="OUB02_002505"
     CDS             complement(706..894)
                     /locus_tag="OUB02_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886254.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            1928..2323
                     /locus_tag="OUB02_002506"
     CDS             1928..2323
                     /locus_tag="OUB02_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886255.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            2366..4465
                     /locus_tag="OUB02_002507"
     CDS             2366..4465
                     /locus_tag="OUB02_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCT8886256.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(4690..9900)
                     /locus_tag="OUB02_002508"
     CDS             complement(4690..9900)
                     /locus_tag="OUB02_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCT8886257.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKRLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDDSDRNYYYFDRYVFNHALNQKQAVDDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKDGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVNLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(9915..12410)
                     /locus_tag="OUB02_002509"
     CDS             complement(9915..12410)
                     /locus_tag="OUB02_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8886258.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLERTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
BASE COUNT         4201 a         2281 c         1470 g         4848 t
ORIGIN      
        1 tgaataatgc ataaaaactt gacatactgt tgtcataatt actttgctat aattaaaagt
       61 aaaaatggag gtaaattcta atgtatgaac gtatgcttga taaaagcaat aaaccctgtt
      121 tgtctcaaat aatagaatat ataggtaaag atggatatgc acgattgtgt aagttggaaa
      181 cttacttgaa aactcaatat aatttatcaa gagaacttcg atttcctttt ggaaacaatt
      241 atggttgggg ttacaaatat agccataaat cttttcattt attttatgct ttttttgaaa
      301 aaggtgcatt tactgttctg attcagatag gtgacaagca agttccagca gtagaaaaaa
      361 cgttggtagc tttgacacca aaagctaaag agctctgggt cactcgttat ccttgtggca
      421 ataatggagg ctggattaag tatcgtattt tagaagatgc agacttactt gatattattc
      481 aatatataag agcaaaaaaa tctccaccta aacaaaatta acttttattt tttgtgattt
      541 aaatatagtg tatataaatt ccattctttt catataaaaa aaataatata aaaaatatct
      601 attagtataa cttatttagt atgtcttatt ctacttttta cagataatta aaaagtttta
      661 tcataaataa atcataacta gacatcctag taaatcaaaa ttaatttatt cttttttatt
      721 agaatttttg ataacagtcc ctatacatat gccaattatc attccaaaaa ttagaccata
      781 acacattgtg atacctacat ttcctttaga aatacctata gcacaaccta tagccattcc
      841 aagtatcatg cctataaaca tacatataat aactaaattg ttgtttttat tcatagtttt
      901 ctccattcaa tatattttaa attgcctagc tcgccttctt agcttaaaac aaagaaatat
      961 aaattaactt tctttcattc taatatctgc ttgtataaat gtcaaacaac acatttcttg
     1021 tcataacctc ctctttttga agtaagtaca atctcccaga cgcaataata tcaatttcct
     1081 aaaaccattt aagtaacttt tatatttaca taatatttac aatcataaac tatattgtta
     1141 ttcatttata catcataaac actccctcct aatcattatt atacttaaaa taatgttcat
     1201 ttataataat atatctaacg cactttaatt gaaaaaacta aaactttgcc tttcattccc
     1261 atatataaat aagtatactt tttgatgctc tattacaaat caaccaaatt aattgatata
     1321 aattatcatt ttctgttgaa aataccattc gcacatgcta tacttaattg cataaatggt
     1381 aataaatgtc atatgatttt acaaatctat taataacaat tattactttt actgtatttt
     1441 aaattttttg gactaaatat caagaacttt ctaaaataca aagtacaaaa aatttatata
     1501 agttataaat ttatatgaac tagataagct caaacaatat gttatgtata aactgtgtag
     1561 tattcggtta taaatttata taaattatat aagctagata aagtattacc tcaactatat
     1621 aagctagata tataaattgc ttatataagc tagataagaa ttatttcaac tatataaact
     1681 acatatataa attacataca taagctagat atataaacta gatatataaa ctgcatatat
     1741 aagctatata agaattattt caaccatata aactacatat gtaaaattca tgtaaattta
     1801 ctaatataaa tcaaataaaa ttactaaact ctctaaacta attttgtaaa acatactcta
     1861 tttttaacat ttgttacctt atacataagt acaaacaaaa tctaactaaa tgaaaggata
     1921 atgtaaaatg aatattttaa aaatgaaaaa aacagggata tttttaggag gagtactttt
     1981 tggtactgta ggtgttaaga tactttcaag tgattgtgtt aaaaaaactt gcactcgtgc
     2041 aacagcagga gcattgaaag ttaaagattc tgttcttgaa acaactacga aaattcaaga
     2101 aaatgtagaa gatattttag ctgaagcaaa agaaatcaac gagcaagaag acttatctac
     2161 atctaaggta aacgttgtac aagatactct taaatgcgaa caagaagttc ttaaaactga
     2221 aactataaaa actgtagaag atactaattc taatagcatt aaatgtgaag aaatcaaaga
     2281 taacgtaatt gaagaaaaaa atagtacttg tacaacagaa taaatttaat gattattatg
     2341 attaataata tcaggaggaa gtaaattgaa tttttatata aaacacgaaa taagaggtcg
     2401 tatacgcttt gacttacaac ttggcaaatt aaacaacaag cagtccgata ctctcttata
     2461 ttatttaact tctataaatg gtattaccaa agcaaaagtt tacgatagaa ctggtgatgc
     2521 agtcatttat tacactggtg aaagagaata cataataaga gaaatcacta aattcagttt
     2581 taacaatagc aaaatagaaa acctagttcc tgaacatacc agcagggaat taaacaatta
     2641 ttatagagac aaattagcaa ataaactgct agtcaaaagt atctccaaaa tattttttcc
     2701 aaagtcctta agattttttt taattggact tcattctata aaatatttta aaaaaggtct
     2761 ctcttccctg ctaaataaaa aaatagaagt ttctgtttta gatgccactg ctataggtat
     2821 ttctattttt agaaaagata tcaatacggc tggttcagtt atgtttcttc ttggtatcgg
     2881 tgaactatta gaagaatgga ctagaaaaaa atctatagac gacttggctc aaagtatgtc
     2941 actaaacatt gaaaaagtat ggttaagaaa aggtgacact gaaatcttaa ttccaatttc
     3001 agagataaag gaaggagatt tagtaagtgt aactatggga aatatgattc cattggatgg
     3061 agttatagta tcaggagaaa caatggtcaa tcaggcatca cttacaggtg aatcccttgc
     3121 agttaacaaa aaagaaggct cttacatata tgctggaact gtaatagagc aaggcaatat
     3181 tgttatgtgc gtcaaggaaa aagctggtac tactagattc cagaaaatag taactatgat
     3241 tgaggaatct gaaaaactaa aatcttctgt agagagtaaa tttgaacatc tagcagatac
     3301 attagttcca tacagctttt taggaagtat attaacctat gcaattacta gaaaccctat
     3361 aaaatcactc tctatattaa tggtagattt ctcttgtgca ttaaaactct ctatccctat
     3421 ttcagtacta tcagccatga gagaatgtaa caataacaac attactgtaa aaggtggaaa
     3481 atttcttgaa ggtgttgcat gtgcagatac tattgtattt gataagacag gcacattaac
     3541 aaaagctcaa ccaactgttt cagatataat tacattccaa aattataata aggaggatat
     3601 gttaagactt gcagcttgct tagaagaaca cttcccacac tccatagcaa atgcagtagt
     3661 ttatgaagct gaaaaacaag ggcttagtca caaagaaatg catacagaag tagaatatat
     3721 tattgctcat ggcatttcaa caaaagttga tgataaaaag gttgtaatag gtagcaaaca
     3781 ttttgtattt gaagatgaga agtgtactat cccagacggt gaggaggata agtacaataa
     3841 tctatcagat gagtattcac acctatttat ggcaatatca ggtaaattat cagcagttat
     3901 ttgcataaat gaccctttac gaaaagaagc aaaatatgta atttcaaatt taagagaatg
     3961 cggaataaaa aagattgtga tgatgactgg agatagcgaa aaaacagcta aatctatagc
     4021 aagtaaagtt ggtgttgatg aatattattc agaagtctta ccagaagaca aagccaattt
     4081 tgttaaaagt gaaaaactca aaggtcgcaa agtaattatg attggtgatg gaatcaatga
     4141 ttctcctgct atttcagaat cagatgtcgg aattgcaatg agtgaaggtg ctgaaatagc
     4201 aagagagatt tcagatatta ccatatcagc agataatttg aataatttaa tcgttttaaa
     4261 gcagattagt aataagctta tgaaaagagt tgatttaagt tctaagttta ttatcggctt
     4321 taatttaggt ttgattttac ttggtgtagg tggatttgtt agaccaagta catctgcctt
     4381 tttacacaac gcatctactg tcggaataag tttaaacagt atgacaaatt tattggaaaa
     4441 caccaatact tataactacc attaatctcc attacataaa acatatgata taaaaaattt
     4501 ataattcatc ttttaaaaag aatatgtctt aaaattagct tttttctaat caaaattata
     4561 taaagcatac tttacaaatt ttaaactcaa cttatttcta ttttttacaa aaaaggagat
     4621 gattcaaatt ttcattttga aacatcccct tttgtaaatt tctttaaaat ttatatatta
     4681 attatatttt caaccaataa ttgtattttc aaccaatctt tcttcatagc attttaaatc
     4741 attttgcttt agataattaa attcagactc tacataaata ccacagccta ccaattcaat
     4801 tgtcaaatta ggtatatttt cctttagtat actagtctgc tcataaataa ttctttccaa
     4861 taaattaact tgtaatatag aaaaatgctt cataatctcc ccatcactat ctatatcttt
     4921 cttgatttca taccaactct ttgacaaaat tcccttcaaa tcaatatacc ctagactcct
     4981 caatatatct atactatcgc tccatgcata cttactaggt ttatcaaagt atgattctag
     5041 tttcttcttc tgctcatttg taagtgaatc ataagaaagc ttcctattaa aatgtgcctt
     5101 caaagaaatc ttagccatag aatatggact tctaccagag tttaatcctg catttttgaa
     5161 cttttcaatt cctttatctt ctactgaaga ccatattgag tttattgaat ctgaatcatt
     5221 gtatttaata tcaagaccat gtgtaatatt tatatagttt tgtggaaata catattgata
     5281 atagacatct tttttatcat catatagaga gaagtatcca ctatcatata aatgaatatg
     5341 cacctttact tttaaactat cctctaaaat atttcgtcta aggttcggga tatttgcata
     5401 catggttaca tattttatgg catcaaagtt tttatttaat ggaacttcaa aactaatttc
     5461 atctcctgtc aaaagctttt tcacttcttc ttcattacaa tcatatagag aatatgtatt
     5521 ttcaaatccc acacgtgatt tcccatctaa actgcctgtt aatgctacct tcaaaatatg
     5581 gttatctgtt ttttgccatt ggtaagcatc ttggtcaaga aactctctaa tctgtctatt
     5641 ttgctctttc gcctgattta taacctcgtt ttcatcttct gtaatcccta catggcatct
     5701 atttaagata tttactatat tgcttccaaa tgggaatgtc catagttgaa aagctgctgc
     5761 ctcaacctga cacaaatatc taacctgatt taattcaatt tgatggataa gttttttctg
     5821 atattcattc atctgattac cactatctga catttgataa atcaattctc ccattgcaag
     5881 attatctttt ttcttatcaa tcttatcagt aatgataatt tgagtttcca tcatatcctg
     5941 tactttttta aatattatag aaatctcctc tgaattttcc atttttaagc ttccaaactt
     6001 tgtgcttaat tcagttaaaa cttccttcat ctttaattta cttatctcat tttcaaaaat
     6061 atcttgattt ttaactgtac tatcgaattt ttctacaaat ggtataacag attctttcaa
     6121 agttttatct ttaaattcca ccatttgagt attcaattca tctaatttct taatattatt
     6181 ttctttatct ttaattgcct ctttaactac ctctatttta ctctcaaaac tagattttac
     6241 attaagacaa ttattaacac cactaatcgc agtatttata agagaacaag cagaacttcc
     6301 aatactcaat gcatttttaa ttttagatgt cttttgctgt ctttcaatag cttgtttact
     6361 tttctctagt gcagctaact tatctttttg ctgtttatta ttgtaatttg cctccaacat
     6421 acctatttct tttttctgga tttttgtgta aatttcctta tactcgcttt cacttaaccc
     6481 ctcttttgct ctttgaatat cgtcagcttt gtctgcatag ttatcatcat ctatagactt
     6541 aactataatc tccttaattt ggtcaaattt agttcttctt tcttcatcaa cacctgccaa
     6601 attataagtc attttatcta aagcattttt tctatcttca cttagctgtt ctccaagtac
     6661 aaatctcata ccatcagaat ccaatttaaa atctgaaaaa tcaaccatcc tgttagtcca
     6721 tttgtattcc tcttctttag ctgaaagatt ttccttcaaa gattttgcac tgtcaaatgc
     6781 ccctagacaa gcttgaccaa caccactaaa tgcagtcagt aaactcaatg cgctttgtat
     6841 tccagaaaga attaactgtg ttttcatttg ttcctcaagt tttgcctgtt tcttttttag
     6901 ttcctctatg tcttcttgtt catcttcttt catctttata atttcttgaa tcaacaggtt
     6961 catttttgca ttaacagaaa gcattttatt ctcaagttca ttttttaaaa cctctatttg
     7021 ttgatttcca gattcaattt gagttaggat actttcctta tatgtattga aatatccttc
     7081 ttttaactca tctttctttc cttgttccaa ttctcttaag gtcttatcca gataatctgt
     7141 caaagaacga atatctgtaa cacgatttcc tccaatttca ctttcttcaa tcatctgcac
     7201 ataaaatgct gtcttctgct caagtgcaaa taaaatcata ctccattcgc taaaattctg
     7261 attttttcct acaaaagatt ctattctatc agactgcatc aatttattta taaggtattc
     7321 atagatttcc tttgcctctt tataaaattc tttctttgta ttcatatcat ccagagaaag
     7381 tactgcttta tttttcttct caaaactata gtacttctga agtatttctc tatttaattg
     7441 catctctatt ttctcaactg cataaaaaat ttcataaata tgtgaaaatt ctaaaatatc
     7501 agcttctaaa attcctactg cacacaacag tctaagattt ctataacctt taattccaga
     7561 aaaaccaaat ctctcgaaaa attcatagaa gaatgtatct attgacaaag aatactcctg
     7621 ctttaacaat tccttttctt ttagtgcatc tccagagtga ccgtcttttc cctctgctcc
     7681 ggctgaacct gaatcttcac gtttgctaat tgtccagtcc ttatgttctt cataaagagt
     7741 atttaatttc tctccatcat ctactgcttg cttctgattt aaagcatgat taaacacata
     7801 tctatcaaaa taatagtagt ttctatcact atcatccatt ggaggagagg tacctatata
     7861 tcttattgtt tcttcatata tacctctaat tccaccaaga cctcctgcac caccagctcc
     7921 tccagagcct ccttttcctg ctaaaccatg atttccatct tttttactta tagcactttt
     7981 taataaagga gcttttccaa ggagataaac ctgtccagac ttgcttccaa gacctgcatt
     8041 tccaccatta cctcctgtgc ctccagttcc accttgtttt cctttttctg cttttaccac
     8101 atagtatttt gtacctttaa cattagcaaa ttcttttgac tcttttttag aacctatttt
     8161 agcatttgcc ccagcttctc cagttatccc tgtatctcct ttttttccat cttgtccatt
     8221 tccaccacgt ccaccatcta cgtaaagttt aagttctcct cttttttcta tttctttaaa
     8281 agataatatg atatcacctg aagtacctgc attatttcca tttcttccat tcattccgtc
     8341 gtaaatagaa ctttctccaa ccaatttttc tgcatcctta ccagatacgt tgattgaata
     8401 agtcccttct ggtatatgta atttttctgt aagtagtaca atattcttac ctgagaagac
     8461 ttctttctcc atattacagt caattttcaa ctcacaatct ccatacatat atatagatga
     8521 caaaatatta agttgataac cagatattat tttagtcaaa tccgataaat tcactttagg
     8581 tataggaata tatatagaaa gtacattttc tttctcatat gattttatat atgtacaact
     8641 ttgaatcttc tctatcctat actctgcatc ttcaagcctt ttggcttctt cttccctata
     8701 ttcttcttct gctatccaat atttgatatc tttcaatgta tcttctaaag tattttttag
     8761 caatgatttt aacaactcac ttagaccaac aatatgagtc tcactacagt aactatttaa
     8821 ctctaaaaga agacgtgttt cttctaatat attcactttc tgtacgccat caccatatga
     8881 aagttcttgt acaaactcca taagctcttt catattatta gtcctactac ataaatctgt
     8941 atacatctta tctaattgaa tctttttatc ttttagacta tttatagaag atagaatgga
     9001 aatagaatct tccatacctt ttttaacact ttctatataa ggaactaaaa gtttttctag
     9061 gtctttatag gttacatcaa cagctttttg tatttctatt ttacaagaag cagtaagcat
     9121 caatcccaaa tctgtttttt caaatgtttc aggtagattt ttcatttcat tcaacaactt
     9181 ttctggctgt ttatctagtg gattacataa tttaatagat acaccatctg ataatgctac
     9241 atttaataag tacactaatt ccttacaacc gtcgtaatct ttctcttgac tatctaggtc
     9301 ttcttttgct ttttccaata tatttcttat tttttcttct gttacattac caattagtgt
     9361 tttaggtatt ttattgataa gaaagaagaa attttcttta tactttgcat aatcaccaag
     9421 catatctttc aaatttttgc ctgtttctat caataatgta tgtctagagt ccagtaggga
     9481 ttgatagtct aaaataacta ccatcccttt tacctttgaa gatgtggaaa tagtttccct
     9541 tacaagcata gcaccagcaa tcatatactc tgctccacgg ttgtcaaaaa aacctgggca
     9601 atcacataat gaaaaatctc tatttgcaat atctacaact tctggacata aagtcttaga
     9661 atatacacca tttccaattt cgacagctga atcttttgca gttatatata tttgtccagt
     9721 ctcttcatcc gttttttctt ccatcacaca gccttttaaa taatttatac aagtactctt
     9781 tccaacgcct gaagcaccta ggaataacaa aattgacttt tcttttaagt catattttct
     9841 aagctcgtct cttccttctt ccaaaaggtt tacgatttca ctataaagtt tatttgccat
     9901 acatccatcc ctttctatat ttcggaacaa tagccttgat atttttcttt aataagattc
     9961 tcttctacct ttgaaacagc tgttgataac ttagtatttg gagcattaaa tcctccattg
    10021 tctcctctat ctccatattc agcgtattct cttgctggtc ctgttttacc ttcattccag
    10081 cttccaccac taaattctat atgatattta attatactat ctaacttata atcccaactt
    10141 gtaaaatatc ctgtatagta tacagtctta tctataccac ttggagaaaa tgaaagtgta
    10201 ttatatccgt gatagccacc acttcctcca tatccaccta aacctggtct accatttgga
    10261 cctgcttctc catctttcac tgttatatca caatgattaa ttattctatt tttttgttcc
    10321 tctgaatctg aaatgaagta aatattacct ttcttaccag catttccacc tcttccagca
    10381 tttccaccag ctccaccttt ttcacctttt ttgccacttg tacctttttc aaaataccct
    10441 ttaaacatct gtccgtggaa ataacttgta tatcctgcat cagctccatt acttccagaa
    10501 gctcctgttt caccattttt cccatctatt cctttagaac catttcctcc atttgcaatc
    10561 agtgttagac gataatttct aatttcttct ttaatatata agtaaatatt acctgcatct
    10621 ctaccatctt caccattttt cccatacata gaattatcta caagttctcc gctctttcca
    10681 cttacattaa tttttacatt actacctaca tcaagattgt tagaagcaag aataatattt
    10741 ttctctgatg caaagttcat tccaaggtta tcatccatat aaatagtatc tttagcaaag
    10801 aacataaatg tagaaacatt gtcatctact tttctatttt tgaaatattc caatacactg
    10861 ctaattacta agtttggaat taatgctctt atttcaatac ctttcttatc attaatatta
    10921 attgttttta ctataaatgg atttaaaatg tatttaccaa taaattctct aattatctca
    10981 ttaccttctt ctccatatgc acttaatctt gttatactgt cttctaactc ttcattttgt
    11041 gtaactgtaa catttgtcaa aattgaatta aaatcactta cactatatac ataatccttt
    11101 ttatttgcaa cttccattaa tttttctcta tttggtaatg taaatacctg tttttgcact
    11161 tcataagaaa ctaaaacttt ttctaaattc tttcttatta taatgaagtt ttgttccatt
    11221 atcttcttac ttgccttttc tataataaat tccatatgta cagaaggtaa taaaatttcc
    11281 ccagttccat gttctgtata tttacttaaa gcatcaatta tatctaagtt tccttttatc
    11341 ttattttgta atttcatgca actatcttgt aagaataagc cactttgttt ggctaatacg
    11401 ttgataaaac tctctatagt aagacttgtt gtactttcca accagttttt aaatactttt
    11461 acaacctcat catattttgc tatttcttga atcattgtta tacttccaat aatattgata
    11521 aaatcttctg tatacgcctt aacataatct tctaggctag atacgatatt gttttgaatt
    11581 tcttttatca acgtatctaa aactaatctt gcatctgcac taattggata ttggaatgct
    11641 gatgtcttat ctttaacttc atctaattct agtacttgct ttaataagcc ttccctctca
    11701 tctgatgcaa gtggcttaca aagatgaata ttttttccat tatcttttag caattcttca
    11761 aataaccaag ctttttcctc tatattactg tctaaatttt cgcataattg ttcatatttt
    11821 ctttttataa cattatatat ttgtttttct gttctttcta agaaactttt tgaaatgaaa
    11881 aaagttatac tacccttaaa attttcttta tgaagacaca tatttaactg ttggaacagg
    11941 tctagtactt ttgtaagtct agaatctaat atatctgctt ctggtatcaa tacaattact
    12001 gcacatatag atgcagaatt tttaaatact tcacctaatg aaactgctgc actaatatca
    12061 tgggctactc ccctattgtc gctaaaacct gcagtatcac ataattttgc atccattcct
    12121 tctattggtt ttctcatatt tacaatgttc gggtataaag tttgagaaac agctcctgaa
    12181 ccaatctctg ccacagggtc taagcaatca atatatgata taccagtatc ttcatcttct
    12241 acttccatca ttacacatcc tgcaagatag ttaattgcag tacttttgcc acatcctgtc
    12301 ccaccaagaa ttagaattaa attttgattt tcagccttat ttattgctac ttttccttca
    12361 tttaataaga gtgttatttc ttctaaaagt tgattgttat tttctggcat ttattttccc
    12421 ccttggttat atattttatt taaataaagt ttaatccctt aagtagatta caaagttttt
    12481 agactatatt taaatttgtt ttttattttt tatttttaat aaatttgctt tttcactaaa
    12541 tttgcatttt cactcaataa ttattcttct ttgtttatta aaataactgt gttgtataat
    12601 ataatcttta ccaacacagt tattctattt aatgcgctta ttttattgta ttttatgtaa
    12661 tacagttgtt ttttatatta tacgtccatt attttcctat ggttattaca tatagatttt
    12721 taattatatt tgtaccatta atttaaacat tcctatacta ctgcttaagt tttacaccta
    12781 taccagcttg aatattttat
//
LOCUS       DAKBNN010000111        12636 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000111 DAKBNN010000000
VERSION     DAKBNN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.112"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(76..1263)
                     /locus_tag="OUB02_002510"
     CDS             complement(76..1263)
                     /locus_tag="OUB02_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8886259.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(1277..2668)
                     /locus_tag="OUB02_002511"
     CDS             complement(1277..2668)
                     /locus_tag="OUB02_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCT8886260.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(3263..4963)
                     /locus_tag="OUB02_002512"
     CDS             complement(3263..4963)
                     /locus_tag="OUB02_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8886261.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYLIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(4935..6338)
                     /locus_tag="OUB02_002513"
     CDS             complement(4935..6338)
                     /locus_tag="OUB02_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCT8886262.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(6602..7093)
                     /locus_tag="OUB02_002514"
     CDS             complement(6602..7093)
                     /locus_tag="OUB02_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8886263.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(7119..7931)
                     /locus_tag="OUB02_002515"
     CDS             complement(7119..7931)
                     /locus_tag="OUB02_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT8886264.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(7944..8903)
                     /locus_tag="OUB02_002516"
                     /pseudo
     CDS             complement(7944..8903)
                     /locus_tag="OUB02_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(9021..10107)
                     /locus_tag="OUB02_002517"
                     /pseudo
     CDS             complement(9021..10107)
                     /locus_tag="OUB02_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10756..11163)
                     /locus_tag="OUB02_002518"
     CDS             complement(10756..11163)
                     /locus_tag="OUB02_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886265.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(11637..12521)
                     /locus_tag="OUB02_002519"
     CDS             complement(11637..12521)
                     /locus_tag="OUB02_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT8886266.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
BASE COUNT         3880 a         2413 c         1340 g         5003 t
ORIGIN      
        1 atcttcaaag tactattttt atcaataaac aaaataaaaa aggtattact ttctttaagt
       61 aatacccatt tatttttaat ttaagtggtt tttcttaatc tcatcaagta gtccatcttc
      121 taaataaatt tctaatgcac atcctaccat tgcctttact gctttgtgaa gtttatcata
      181 agcttcctca gaatttgcat aatttagaaa ttctttttca tgaacatata ccattggact
      241 tatatctaag gctatttctg tatacatagt agggcaaact tgacttacat tacctatatc
      301 tgtagaacct actggaccat ctttaccttc taatatatct gttatacctg cctcaatcaa
      361 attattcttt gttatctttt gaagtacttt gttatttatt atgttgtcaa aagaattttc
      421 aaagtatcta taagaaagct tagctcctgt cattaactct gcaccttttg cacagtttat
      481 aactttttta gttacttcat ctaaataact tctttcagca gctcttacga aaaacttaca
      541 ggctgctttt tctggaacta tatttggagc ctctcctcca tttgaaacta taccatgtat
      601 tctaacatct gattttacat gttgtcttag tgcatttatc ccagcaaaag taagttgtac
      661 tgcatctagt gcattgactc ccatatgagg gtgactagct gcatgtgctg ataccccttc
      721 aaaactaaac tctaatgaat ctatcgccaa agccttacag tttatattat taaaagcttc
      781 tatatgcatt tgataactta catcaacatc atcaaaagct cctgccttaa ctaaatcaca
      841 ttttcctcct gttgtttctt cagctggtgt tcctattagt acaatttttc ctttaaaatt
      901 ttcttttaac ttacttaata ctatacaagc tcctagtgta gatgctgcaa tccagttatg
      961 tccacaagca tgagctggtt ctttattttc tccatatcca ggaagtgcat catactcagc
     1021 taaaaatgct atggtaggtc catcattatc tcctaattca gctctaaaag cagtctccat
     1081 attacaatat ggataaactg tatcaaatcc atattccttc attttttcta ctaagtattt
     1141 tgatgatatg tattcttctt cccctaattc aggattgtta aatataaaat tacaaacttc
     1201 ttcaatttct tttccaagtt cattctctat ctcataagct ctcttcttta tactctctat
     1261 cataatttac ctcctactag aatcctagat ataatcctat tgtcaaaaat gccattacaa
     1321 gaattgttaa tactaccatt aaaggaatta cgaatctaac ccactttgac caaggaatat
     1381 tggctactgc tagtgctccc attaaaacac ctgatgtagg tgttattaag ttagttacac
     1441 ctataccaaa ttgataagct gtaactatca tttgtcttga tacatctact aagtcgccaa
     1501 gtggtgcaag tacaggtatt gtaagtgatg caagccctga tgctgatggt attaaaaatg
     1561 ctattaatcc ttctattaag aaagttaaat ttataaatat agtttttggt aatccattta
     1621 ataaatttgc tgcagaattt aaaattgtat ctattataaa tccattttct gctattatta
     1681 cgattccacg tgcaaatgct atacacaatc ctgctgtaat taaatctcct gctccactta
     1741 taaatgaatt aactatctca tcttgtttaa gccctgatgc tattcctgat attataccta
     1801 tagctgtaaa tatcattgca atttcagtta tgtaccattc tagtcttaaa actccatata
     1861 tcataatcgc catacctatt gtgaaaatgg ctattaccat ggcttctctt tttgtaaatt
     1921 cctttatatt tgtatcacta ttaaccatta attcttgatt tagtgatata tcgtgtacta
     1981 aagatttttt agggtctttt ttaactttat tggcatacat cataacaaat cctatagata
     2041 tagccatata tattataaac ataacaactc taaactctat tccagaacct ggtactattt
     2101 gtgataatgc ttgtgctatt cctacagaga aaggattcgt agtagctgct ccaaatccag
     2161 ctgttgctcc tataaatact gtggccactg ctgtcaatga atcatatccc atactggtca
     2221 taagtggaat aaatatcata taaaaaggta gtgtctcttc actcattcca aacgtcgtcc
     2281 cacctagtcc aaatagaatc attgaaatcg gtattattaa aaattgaaac ttcttcattt
     2341 tatttacagt atgagctata acagcttcta tagctcctgt tttattaact atcccaaaag
     2401 ctcctccaac tattaaaaca aatccaacta cttcagctgc atctataaat ccgtttatag
     2461 gtgctgtaaa tacatcagta attccttgtg gatttgatgg tgctttttca taagttccag
     2521 ctacaacaac tgaccttccg tctatatcta ctctttcaaa atttccactt ggcactatcc
     2581 aagtaagaat tgccattaac actattagca aaaatattat tgtaagtgtc tgtggcatag
     2641 taaacttttt cttttttaaa ctgctcattt ttaatctccc cactttttaa attaaatatt
     2701 ttattattgc atctctttat cctattttgt atagatgtaa aaaagctcaa catggataca
     2761 tgttgagcta aatgtgctaa atacaacata cctgcatctt ccctttatct tgtttaagat
     2821 agctctccat caaaattcgg gataattttg atgacagtac tgtacctatt aaatacagtc
     2881 ccagcactag cttcggacac tagcacttcg gcacttactc cttttatatt gcctcattgg
     2941 tttccaacct cttcaatatt aatcttagta aatgcaacct ctacctcaat ctgtttgtta
     3001 gatgaggtaa ttcttattaa tttttattaa ttcaaagtaa ttgtagcata atatgtcgag
     3061 attgtaaata ttttcagtat aataattctt actttatgat ttcattccat ttttcacaaa
     3121 taattttttc tccatataat ttaaatactg tagtatattt taaatacaca caaactttcc
     3181 acaaatttta aattattata atacctttat aataaaaaat aaaagctatg taaacttatc
     3241 aatttacata gctttatata cattaatcta ctccaagtac agctttaagc tttgatgcta
     3301 ttgtaataac tcgtggacca aatataactt gaactgagtt tttcttttta acaactccac
     3361 ttgcacccaa ttcatttacc cagaagccat cttcatagaa tccatcaatt gaatttagct
     3421 ctaccctaag tctagttgca cagtttgtaa cattttttat attattactt cctcctaaac
     3481 cttctattat agaatgtaca agctttgtat ctttatcatc tacttcctct tctactgcat
     3541 tgttgtatgc tgttttacca gcatctacag attgtctttc tctatattct ttcttactat
     3601 aaagcttaac ttcttctgaa cttcttcctg gtgtttttat atcaaacttc ttgattgccc
     3661 atgtaaatat tatataagta actataaaaa atattggtat caaaaataat aatggtaacg
     3721 catccacttt ttgtggttgt aataaattag gaaacatatt tctaaatgat tctcccatta
     3781 tagaaacatt gaacatttct gtaaatacat acgtcaatcc tgccagtggt acatatgcta
     3841 caaaatacaa taatggtgca acaaatagga atgtatactc tattggttcc gttaccccta
     3901 ccaacatggc tgttactatt gctggtatca atatacttat tgcttttttc ttattagcat
     3961 tatcagccaa cttatacata gctaaagctg ctgctggtaa tattgcaaaa tgtattaata
     4021 aacgacctgt tgtgaagtta cgagttatat aagatagtgt atctgggtct cccatttgag
     4081 ctaatcttat attattaaca ccctcgtaaa ttgctccacc tacttccatt gttccaccta
     4141 ctcttgtata ttctataggg aatgcaatta agtgatgtat accaaatggt aataatgctt
     4201 tatccattgc tccaaaagta aatgttccta ataatccact agaacctatc caattaccaa
     4261 gtttagataa tgccaaccct actggtggcc atataaggta cattgctgta cccaatacta
     4321 ttgatgctat aaacatcata atcattgaaa atcttggacc tgagaaaaaa gataatgcac
     4381 taggaagttc tatgtcaaca aatttgttat gtactgcact agctacaaat ccagctatta
     4441 tagctccaaa tatactacaa tcatatgaaa agattcctaa gaaagaacca tataaagaac
     4501 ttcgactata tgcttctgcc tctcctaatc cagatgccat aaacgcttct gctgtaactg
     4561 tatctgaatt tataccattt gctgctaaca atgtacttat aactgtgtgc attcctataa
     4621 ataaaacaat tcctcctaat gcaccccatc ctttttcctt tttagataat ccaaaggcta
     4681 tacctgtaac aaagaatatt ggtaaatatc tcattaccat aaatcctaaa tcgtttatta
     4741 ctttaaagaa tacatttata aatgaccctt cttttattag tccgctaagc cctaatgcag
     4801 aaacgttttc catatttgca aaagctgacc ctaatcctat aaatactcct gctacaacaa
     4861 gtaatataac aggtatcatc attgctccag aaagtacttg taatttatcc ttcatagtat
     4921 tatttcctcc atttttattg aaatgccagg ctatataatg catatatata tacttctatt
     4981 gctttgtata atgtatctat acttatattt tcatttgcct gatgtgaatt tggattatcc
     5041 ccctctattg tgggtccaaa tgcaactcca ttttttaaca ctcttgcata agatgcagct
     5101 ccttttgtaa aaagttcggc ttcttttcca aaaccttgtt tatatgcact tgataattta
     5161 tttattaatt cactatctgg ttttacatac agtaatttta aatccttata tatatcaact
     5221 attaaatttt ctttttttcc aatttcatta attctattag ttataaactc ccaacttata
     5281 cccttaggat atctaaagtc tattttcata ttcaattcat ttttctctaa gaagatactc
     5341 attatacata ttgtagaaac tcccatatct acatcttctt tatatatccc taattttttt
     5401 ccatgattat catctgtaaa gtatgaattt aaaatatccc ttattataac gcctttatca
     5461 tttaattttt taattttctc taaatctttt gctaaactaa atgcacagtt atatgacctg
     5521 tgtggatttc tcgataaagc cctttctcct gtatatctta ctaatacttt accttcttct
     5581 agttcttcta tctctgtata ataagctaat gattctacta aatctttctt atcattagtt
     5641 ttaaaaacag cctcaatttt gtcacataca aatccatctt ctttgtttga ctttatatca
     5701 actaaattgt gatttctaaa tttatcttta tctattttct ttcttagatt gaagtataaa
     5761 ataccttttt ctccatttac tattgggaag tttccatctg gagaaaatgc aaattttggc
     5821 tgttcattga cactaaagta atgctccata cattcccaag tcgtctcttc agctccaccc
     5881 actatcaacc taatctttct cttcggcttt tcattaagtt ctcttaagaa aagtaatgca
     5941 tataaaatcc ctatcaaagg tcctttgttg tcattaactc ctctaccata aagatagtta
     6001 tctttttgac atactttaaa aggtttactc ttccaaagtt ctttattata tattggcacc
     6061 acatctatat gattaagcac ccctactact tcttctcctt ctccatattc tatatgaatt
     6121 gcatatccat caaagtcttt tacaacaaaa tctttgtctt ttgctatttg aataagctta
     6181 tcgaaggcat ttcttatttc tatcccaaat ggttgattta tatctgttgt actttcatct
     6241 ctaattgaag gtatttcaac taaacttcta atatcattta tgatattttc ttgattggat
     6301 tttaaataat caataattag ttctttgttt actatcatct taattattta tcaccccttt
     6361 atattaattt tacaaagcat ttcagttcat atctacatta taaattttga atattatttt
     6421 ttcaatattt caaatacttc tctaagttac cataatgata cggtaaacaa tcaacagatt
     6481 atgacaaaat catactatat ctattactaa tattttaact tatctattac gtctttttta
     6541 atgtaagttt attccaaaaa tacataaaaa aagatgtagc cttagctaca ccttcttata
     6601 tctattcaac ttccattatt atgctattaa tatctacatt tccaggttct atcctatctt
     6661 ttactgttct cttaacagaa tcagcatcat ccattataac tacaggagtt attatattta
     6721 aatcatttga cgttataaaa tctaaatcta cttccattat taaatcttta gcttttacct
     6781 tatctcctgc ctttacctta acatcaaacg gctttccttc tagtgacatt gtctctaaac
     6841 ctacatgtat taatatattt gtattatcat tactcttaat cattattgca tgctttgtat
     6901 caaatacatt gactatttca ccatcaacag gagaatataa cttaccttca cttggtatta
     6961 ttgctactcc atctcccatc atcttttggg caaaaactgc atctggaaca tcttctaagt
     7021 tcactaaatt tcccttaaaa gggctatata ttttctcaat cttattttct tttttcttaa
     7081 atatattaaa cataacacac tctccacatt ataaaatttt atgataatat ttttcttaaa
     7141 gcttttgcta caccatcatt tacattagta tcagttacaa aatctgccat agattttact
     7201 tcatctgtag catttcccat agcaatacca agacctgcaa atttaatcat ggatatatca
     7261 ttttcattat cacctataca aataacttct tcgctatcta tacctaatat atcacaaaat
     7321 tttttaactg caataccttt tgaagtacct ttgctcataa cttcaaaatt tatctttcca
     7381 gaacttacta cttctaaatc ttcttgtttt ttaaattctt cctttgcttc tctcaaagaa
     7441 tctacgtttt ctgagaaaag tatacatttt aaaatttcat tatcagcttt ttctaataaa
     7501 gtttcacaac ttgcattttc tattatttca attctgtctt cttctttatt tttactattt
     7561 attaatctat atggatgttc ttcacctatt tcattgttgg atataactat tttacagcct
     7621 ttaaaatgtt tgtttaaatt atacttttca cctattgtat agattttctt tagagcttct
     7681 ttgctcaaaa ccttcttata ctcatatccc agtaatttaa aatatgtccc attagttgat
     7741 ataatatata tatcatcccc taaaacactt gtaaaatatt tcatagcatt ataaggtctt
     7801 cccgtaacta aggctacctt gatacccttt tccatgactt cttttagtgc tttaatattt
     7861 tcttctgata cttcaaatcc tttacccatc aaagtaccat ccatatcagt acaaattaat
     7921 ttatatcgca tttgtatcac atcctagtaa gatttttcta atatttcaac aaggtcttct
     7981 ttctttccaa aaggtaaaaa tcctaaaact ggacagcttg ttatttgttc taattgttcc
     8041 tttgttacat taaactcaga catttttgtc ttaagtccca ctctctttaa aaaactttct
     8101 atttcatttc ctaaagtttt agctgcttca tcatcttcca ctttttctaa ttcttggttt
     8161 agcattctcg ctactttggc aaatttctct atattatcct tatagaactt ttctgcaaat
     8221 tttgggaaca ctatagccaa agcctcacca tgaggaatat gtgtaactcc tcctattatt
     8281 tcacttagtg gatgtggtgc tgctgctcca gcatttgcca gtgaatttcc tcctaatgta
     8341 tctgctactg ctaactttgt tctgtattct atattatttt tatcttccat tactttaggt
     8401 agatagttaa tgactaattc catagatttt tcagacatta tctctgtaaa atcattagca
     8461 tttggattta tataactttc aaatgcatga gtaaaagcat caaatgctgt tgatgcagta
     8521 attctctctg gcagagttaa cataagctct gggtcaacta tacattcttt tgagaaacaa
     8581 ttttgatgaa atattgtatt tttctcgctt cctctcgata ttactgctgc ttgtgtaact
     8641 tggcttcctg ttccagaagt agttgataca gctattaatg gtaattcttt ttcacttacc
     8701 ttatcgtata ttttaaatgg atttgtatat gtactaaaca tataatccca atctatcttt
     8761 tctaaaccaa aagtcaatgc taatactttt gcagtatcta tacttgaacc tcctccaaca
     8821 gctaaaacaa catctacaga ttctttttta gctaattcaa acccttcatt tattaattct
     8881 acagttgggt ttggctctac tctctgaacc tcccatgact aaagtcacag ggttcctgct
     8941 tcacagaatt ttgcatacta aaatatgtct tactaattct ccacaggcca tccccgtagt
     9001 tcctacggtt cttacataag ttatttattt tgaattgtta ttattcttaa tccttctttt
     9061 aatatattta tacttgcatt tatatctcta tcgtgagttt cattacaact aggacaaatc
     9121 cattctctta tatttaagtc cttcacttcc tcgtttttat acccacattt attgcatatt
     9181 tgtgaacttg caaagaattt acctaccttt acaatttgtc taccatgcca attagccttg
     9241 tattctaatt gacgaataaa ctctgaccat gatacatcag aaattaagcg agatagttta
     9301 cgatttctaa tcatattctt tacttgtaaa tcttctatgc aaataatatc attctctttt
     9361 attaatttag tagatagttt ttgtagaaaa tcatttcttt gattagctat atgttcttga
     9421 agtcttgcaa cttttaatct tgctttattt ctatttaaac taccgattgt ctttctcgat
     9481 aattctcttt gtaatttagc aagcttgttt aatgacttct taagatactt aggattttct
     9541 ataaattctc cacaacttga gatacaaaat tctttaattc ctaaatctaa acctatctga
     9601 ttattagtat tttcaaatgc ttcaatatct accacttggc tctttagata tagtagcatt
     9661 aagtattctc cctttaggta cttgtttatc ccttacctta accataccaa gtttaggtag
     9721 ttttatatgt tgaccacaat acataatgtt tccatttgta aagttagttt tatatgaaaa
     9781 tctattaatt ttctttgatt taaatttagg aaatcctgct ttttctttaa agaatttttt
     9841 atatgcatta tctaaatctt ttaaggcgtt ttgtagtgaa aatttgtcag gttctttaag
     9901 ccattttaat tcttttttta aattggtcaa atcagaacta cactgtttat aagtaaaagt
     9961 ttctttattg tttttataaa catctattct tttagcaaga tacttattat atacaaatct
    10021 acaacatcca aaagttttgt taattaactc ttgttgcttt ttatttggat acagtctaaa
    10081 cttataagct tttttcactg ctatcacctc actttaattg ttttttgata cactttttaa
    10141 tgattttatc acattttatc acatttaata tattatataa tgctgtggta aacaatttat
    10201 taatcaccta taataatgtt aacaaatgta attatttaaa acaattattt agaaggtttt
    10261 atatttaatt ttaaataaca tatttaagaa ccgtaagtct taacttttat catttttgag
    10321 gttgtcgttc acataagttc gctactacct atgcagttct cttatgaact tcttacactt
    10381 tcatgtaagc acagactata tcttatcctt cagcattatc cgttaaggtc tacccacttc
    10441 cactaccaat agcttgtagt gtacttccct caagaggaat agtcgttgaa gtttctccta
    10501 ttcggagttt acctgctgat tgcccatttt aaagtactta ggatttaacc ttataccatc
    10561 taaccaattt tttctacttt cgtaactttc acacttaggt ttatttcatc cttatgtttt
    10621 agtttgatta gctttagggg tttccagcaa ttcaagtagt attggatagg ttctatccta
    10681 tctctacata taggtttccc tatatgctga ctattttagc tactaactca tgactaaagt
    10741 cacgagtgtg cgtagctatt ttttaatcaa tattttagta aattttacgg taagtaaaaa
    10801 cctctctcct tcaaatccta tagcaaatat acttacaagt tgctttgaga aaataactcc
    10861 taatatagct acaactatgc atataccaac tattatattt aaaacattgt ttaaaaactt
    10921 tagtgcttgc ttttcacctt gtttactact tatatcttga tacattggaa taaaagtagt
    10981 aactatagca gtacctattg ctgcaaatat aatatttggt atattcatag ctgttaaata
    11041 actctctgta tataagcctg taccataaat tgatgataat actaattctc tacccattcc
    11101 aagaatctta gagattatag taactatcat caaataaaaa gttgctttag ctaccttgct
    11161 cattttatca ctccacttct aacatacctc tttttaatca agtaaaattt agctcattag
    11221 atataattct atcaactaat ttgccttgct tagccctatc aaaatataat taacacctat
    11281 ttcttaatat atcacaaact acattattct tcatattttt ttatttctcc aataagtttt
    11341 acatttttcc tatataaatt gtatagcttc aattatattc aaagaaatgg agttgctacc
    11401 cgataacttt aaaagtaaaa aacagatttt attatctttt taaactatat tctaacatat
    11461 tttgtccata ttgagaaatt ttactaaact attatgtact ttattaaata aataaaataa
    11521 cagcacacct ataaaataca agtgtgctgt tcataaacag attttataaa aaaataattc
    11581 ctaagtagag attaacatgc tttctcatac tctcatatat aatgtgtcta aaaatattaa
    11641 taaaaatgct ttaattgttt taacttactt ggatgtctta attttcttat tgctttagct
    11701 tctatctgtc ttattcgttc tctggtaact ccaaatacct ttccaatctg ttctagtgtt
    11761 ttagggtcat catcatcaat tccaaatctc attcttaaaa cttgctgttc ttgctctcca
    11821 agacctgtaa gtagttcttc tatcttttct tttaaattat gctgttcaac ttcgtgtatt
    11881 acaacatctt taaaatcagc atctgatggt ataaattcaa ctaaactact atcctcatct
    11941 tcttttaatg gagtatccaa agatacaaca tttttatctc ttcttaataa ttccaacact
    12001 ttgtcaactt ctaagccaca tgcatctgca atctcatcta tagttggctc tcttaataac
    12061 acatttaaaa gttcttgttt ctttttcttt acaaaattta ttctctgatg aagatgtata
    12121 ggtatcctaa tagtattttc gcaatcgtcg atatatcttg ttatactttg ttttatccac
    12181 caagttgcat aagtgctaaa tttgtatcct tttttataat cgtacttttc tacagctttt
    12241 ataagtccta tatttcctgc ttgaattaaa tccaacatat caatactatc tcttttatat
    12301 ctctttgcta tgctcactac caacctataa tttgaattta taaatttttc ttttgctact
    12361 gaagatgagt tgattatttc cttagccagc tctacctctt ctcctgctga caacattttg
    12421 tattcctcta tttcctttaa atacattttc atcgcatttg atacatttga tgaacccaca
    12481 caaatataat tttcataatt attttctttt ttagtatcca taatcaaacc ccttctattc
    12541 atagaatttg ctaaaacaaa aataaataat ttaaacctac ataatctaca attacataat
    12601 atcccattct ttaaatgaat tgttttgtat attatg
//
LOCUS       DAKBNN010000112        12595 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000112 DAKBNN010000000
VERSION     DAKBNN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.113"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(94..1974)
                     /locus_tag="OUB02_002520"
     CDS             complement(94..1974)
                     /locus_tag="OUB02_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HCT8886267.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISIKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTLDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2184..8147)
                     /locus_tag="OUB02_002521"
     CDS             complement(2184..8147)
                     /locus_tag="OUB02_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HCT8886268.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNIQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNFTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKISFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPINN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIMVDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8271..8356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8528..9931)
                     /locus_tag="OUB02_002522"
     CDS             complement(8528..9931)
                     /locus_tag="OUB02_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HCT8886269.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10393..12288)
                     /locus_tag="OUB02_002523"
     CDS             complement(10393..12288)
                     /locus_tag="OUB02_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HCT8886270.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGLSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         3468 a         2323 c         1408 g         5396 t
ORIGIN      
        1 ttagattgtt aagataatga aaataaaata agaatgcatt taaatatata actttatatc
       61 taaatacatt cttattttct atttacttaa tatttatttt tcaattaaac tcttaatctt
      121 gtctattaca gatgaggcta ttcctccacc tacttcaaac aactttgatg aatttttacc
      181 ttttaatgac gattcttgtg atttatctaa cgtatttgta gcaagtataa ctggtgaatt
      241 gtttttagct gctaatggtc caactgctaa agcatctata agtttatctt gatttgcaac
      301 tacaactcca tctagcttag attgagagta gaatttatct attactttgg cattcgtctc
      361 ttgtctagtt tgacctgcca ccctattgtt tgatatatct ttgtttgcta tttttccaac
      421 ttgttcaata gccttgttag atattttttc tactccacca ataaaatata tatttgacac
      481 cttatttgat ttaataaatt gttcagcttc attatctaca ccatcttttt gtgtaagtac
      541 tattggagtt ttttctttac cagccagtga tgctattgat aatgaatcag cttctccatt
      601 tcctgctcct atatacaact tgcttacatt attcgtctta tctatttctt ttgctatatt
      661 aatagaagtt tgatatcttg tatctccacc tattctttgt actgatattt ttgaatttac
      721 agctttgata gcttcaacaa ctgaatttgg cattgagttg tttccaccta tcacaattac
      781 ttttgatgga tttagccttt tcaattcttc tacagttttt tgaggaagct tttcatttga
      841 tgataaaaga attggtgagt tttttacact agcaagtggt gtagctgtaa gcccatccac
      901 catactttta tcttctccat ttactattac aactgtatct gattttgtcc aaccactttg
      961 agatattttg atagctgtct caaatctatc agcacctttt aattttttga ctgtgacatt
     1021 agatgtagaa tcacttgaat ttcctcctcc agagcttcca ccactagatg agccgcctga
     1081 attacttcct ccgccattat ttccaccatt gccattatct ggtggtaatt catccggttt
     1141 tgataattca ttttcaacta tgtattctaa actttttgga agtgtaactt caacatattt
     1201 gtttattgta gcttttcttc ccttagttgt tttttgtgct aatccaagtt ttaatattac
     1261 atttgaacca ctttcttgtg tttctttact aacaatagaa tagtttatcc taatattgtt
     1321 taataaatat tcaagtgctt cacttttctc taccttatac ttttcagggt cgtttttact
     1381 agaaccagtt ccaccaatcg gttttacttc actctcatta ataagggtaa atattaatcc
     1441 ttcatcagag cttatatttt cataatccaa tgttttgttg ttatatgtta caatttttaa
     1501 agctggttta tcgcttgttc cttttcctaa aactttatag tcagcatttg agttactgcc
     1561 agattttttt atagatattc ttcctatatt caatctattt ccgtctagta aatcttcatt
     1621 tgatgtaaca tatatatcta atgtagttgt attacttgat ttgttgtatt ctgctctaca
     1681 agtagcattt gcatttccac tttttatctg agattttata ttatcagacc aagtaatctt
     1741 ttctttctgc tcattatcta ctacttcaac ctcaccttgt acttttatac tggcttgaaa
     1801 tgaatatact atgttttcta ctgagtccac agatatatct atagtattta ctttctcata
     1861 cgtttttgtt ccttgtaaaa ctggagctgc atatgcacat gtagcactac cagttacagc
     1921 tactgccata gttgatactg ctatagttct tataaaagat tttcttttac tcattaacac
     1981 ccgtccttat ctatcattaa ctaagtggat aaatttactt atccacctag cttttatatt
     2041 taatttataa attaccctaa acttatagtt acatttatat actttacttt taatatctac
     2101 tattttaaaa ctattctagt acctaatctt attagatata caatgttagt gttagtattt
     2161 ttacgttttt agagttccac ctactattta tctctaacaa tattaccctt tactttattg
     2221 cttacagtta taaccttctt attactgata tcaatagaat ttcttgtagc ttttctagct
     2281 ttactattac tatcagtaag acttattgtc tttaactcat cataattctt agctcctaca
     2341 ggtacataca ttgtatcact tacaaatctt tgacctactg gagtatctcc ttgaaggtca
     2401 ttgtatctat atagttcagc cttaacctta tcacttaaag aatcaaagtt ttcttttggc
     2461 atatagtaaa cccatgtacc tttactgatt tcacctagtt cacctttttc tggaacttct
     2521 gcaaataaaa gcgcttctcc agatatagtc ttattgtttt tatctataag tagtggtaaa
     2581 ttaaatgctg gatttccact gtattcacct gtaataacta ttgaacccgc tggaatctta
     2641 cctgtttcat cttcacttgt gctagagtca tcactttgat atacataatc ttctgctaat
     2701 tttccaattc cattagtacg agtttcgtct gcattattca ttgtgccaat ttctatatta
     2761 tcacctggag gtcctattat atctaattct gcaacttcta tattagactt attatttgag
     2821 attagtttta catatctagc ttggtatgtc caaagtttac catctttatt aaatgtaact
     2881 ctagcttcat tatcttggtc ttttcctcca ccaactaaat cttgtccata ttcaaaagtt
     2941 ccttgactta catcttccca gttcttttta tcattactta cttgtatttt gtaattagtt
     3001 aaaggattgt atgttgtgct agtatttttt ctaaagttaa ataatccttt taatgagaac
     3061 ttcctagctt ctgcctctgg ttttgtatat tttagtccta ctataggtaa tgtttctttc
     3121 atgtctacca ttatatatgg attttcttct acatttatat cagggtcttt ttgagggtct
     3181 ttgttccact ctgctttact tattatcttt cctttatatg tagtacttgc atcattatct
     3241 tttatattat caatttcagg attttgtaat ggtccatgtg agtcattttc agttctaaca
     3301 tcaccgtcag aaacagtatt tgttatgaat gaccatgctg atttatttat tttcccttca
     3361 tctgataatt ttacagtacc aacttcaaat tcttctgttg gatttaggtg ataatcatat
     3421 gcaactacct tatatgtaaa tgctctgtta ttaagaggag atatattatc agtaaattta
     3481 gtcttactat ttccttttgc atccacaaat ccaactgttc tgtattttac ctgtgttcca
     3541 ttttcaccat ctaaataatt tccatcactt cttattattt cgtatcctag tatcttatct
     3601 ttatccttat ctacatctag gtctatagat attttattag atttaactag agaaccactt
     3661 tgtattccat cagaaaaact agcacttact ttagtatctt ttgccatact agctatattt
     3721 tcatttaaaa tctttcttct agcttcgtca tttacatatt gtatctttct tgattctttt
     3781 tcgtagttct tactatccat tatactgttt aattctggtg taatttctac tccccatgcc
     3841 ttaaagtaat ctcttaaatc tttttcaaca acacttgatg ccattgctac tagcatttgg
     3901 tcttttggta aactattaat tgggtcacta cttgaaagtg ctctgtattt tcttgaaatc
     3961 tttgcataga atgtatcatt acttaaatca gcatctgagt tattctttaa cattgaaccc
     4021 gtaaaatcat catcataagc tagatgtaat tgccataaca ttccaagcct ttcaaataca
     4081 ttgcttggta tacctaagct atttgaagtt acatgctcat atatcttatc cattgttgaa
     4141 ccttcaagtc ttgaatgtgt cttatcatca aaggtctgag caagtaaagc aagtacattg
     4201 ttactagttt cgctatatgt catctttcca atatctgtaa catgtcctat ctcatgacct
     4261 attccccatc caaataagtt tcctcctgta gcttttccac tatcatctat ttggaaaggt
     4321 actccattca taagccctgg aactgaacca tattctactc ctacatgtaa tccagaagca
     4381 tacataaatg ctccaataaa cattctttgg tactttatgt tcatacgaga ttttggagcc
     4441 atatcatttt tagttaagtt aaattcatta tcatctacag ttccattacc atctaaatct
     4501 tgtgttttaa atactcctct ttctgagtaa actaaatcca ttatttgttc ccatgcaagc
     4561 atactattat ataatctatc tacttgtgta tttaaatcag catttccttt tgatatccct
     4621 tgatatacag ctgttgcagg aacatttaat gttactttat cactttcaat atctgtagtg
     4681 tttaacacac cagttttttc tgaatatcca tatgaattac caacatttgt tcctggataa
     4741 tatggttctc cttttacagc atctttatat ttagctaatt cttctatata tgcttttatc
     4801 tttttcttag attcagaaac tcccgcagga ctcttatcat ttattgttcc atttagatta
     4861 agatgtggta tcttagtagc tcctgctaca cgtactttta tatctcctcc acttggagta
     4921 tcttcagcgt atcttatgta taaacttcct ccacattcta tatctaaact atgaagtttg
     4981 cttaattgaa ttacattttt tccagtgtta agatttattt ctccactctt atatgcacca
     5041 gattctccat agtactgtgt atatatcaac ttaggagttt ttccactttt acttccaaca
     5101 tatactgtga tttcatctcc tgctctagca gctactccta atgcctgcca gtcattttgc
     5161 atacctaaat tattggaatt tacagtatac tgtccatttt tatctttata ttttcctaat
     5221 ttatttattt cttgatttac agtagttaca tcagtaagtg tggctctatc attatataag
     5281 tcttgagcta attttaattc tctctcaata actgtcttat atggatgata ttctccacta
     5341 gctttatctg gagtatttaa tctatctttt aattcagtta ttttttcttg agttacatct
     5401 tcttttaatt caacttgtag gtcatccata aatagatttc ttacatcatc ttctatactg
     5461 tcatactcat aaaacttaag ttctgatata ttagacttat atgaatagga tgagcaaaca
     5521 cttacttgta ttttggtagc agttattggt tcttttaatc taaccattac atacttatta
     5581 ttatttatag ggtctgtctt tgtttcaaaa ggaacatccc ccagattgtg taattgacca
     5641 ttttcatccc aatatcgtac ctcaaagtca tttattgtct tatcagaaac atcaaaacga
     5701 ggtgtagcca taaaagtatc catagtataa gatttatcaa aagttattat tggtcctcta
     5761 ttcgttccct cagctgcatt atatgttggg aatgtccaat gtgttgtaaa gtccccatct
     5821 actacattga atttatcaaa tggattttta tcactatatt cacttttgtc atatcctgct
     5881 ggataagtaa catctacaat gtgatttgtt ggtattccaa atccaccatt ctctttagga
     5941 gtattaatta atctatagtt aggtgttgtt ggtgcatcca tgctttcagt tgtacctata
     6001 tatgttttag acattccact agttcccaag tggttagtag ctgtcattct aacttcatat
     6061 gttgtattat ccttaagacc ttctatcttt gaacttgtcc cacgaataag cttatcattg
     6121 tattttatcc aagacccatt agaacctttt tctttatagt acaagtcaaa gtctttagct
     6181 tgtttatgat ttttccagct tacatcaaga gatttaaatt tacctttaac atttatacct
     6241 tctggtggtt ctggttttgt gtctggaaca acttgaaatt ctaatatact atctgggcta
     6301 aattgtatag gtttatagtt tgaatctaca ttttctggta ctccgtctaa gttttctggt
     6361 gttgcatctt tataaccact agaccaatct ccatttaaag attgtatgct aaccctatat
     6421 agtccatatg cttctatatc tctaacttct attgtatttt tactagtttg taatttttta
     6481 gtagataata cttttccctt actatcaatt ttgtctagta taagttcgta tgctgataca
     6541 tttacttcat tattccaata aaaagatatt ttttcatttc caacagcagt gctagtcgat
     6601 attttattta ttttaggtat attcatctct ggttttggta tttctttata tacattgttt
     6661 aaaaattcaa cttttgatat ttctgcaaga tttctatcat ttgctgttgt agctgttatt
     6721 tttatagtta tcttttttac tgctacttgt ttaccaagat ttattactat atcactattt
     6781 tgaacatctg catatgtttc taatgctgac ctactatatg tagatgttgt aaagttatca
     6841 tctttactga tattatcccc ttcttttgga gtatcttcat ctgaattagg tttatccatt
     6901 gatgaatttt ctttatcatt atttttatct tgattggtat tagagtttgc ttcttgttta
     6961 tcgctatttt cctgttgttt atcattattt tcatctttat tatttgagtt attatcagtc
     7021 tggtttatat tttcatcttt attatttact tcttcttgat ttacttcttc ttgttcgtta
     7081 tttacttcct cttgttgctc ctcatctttt aagtttttca tatattcttt gttttcataa
     7141 ggaatctgtt gaatattacc atctttgtca gtaacctcaa ctattccttt tgctggagca
     7201 gtaccttctg gagcttttat attgatgact tccatattaa gtgcttctga taaagctata
     7261 tccattactg ctggtttttc ctcagatatt tcatcatttt ttgaagatat tgtaactcca
     7321 ttattttctc cactaaataa attttctaca ttaccattaa tatctacatc actatttcct
     7381 tttaattcaa ccttacttgt atctactata ggattggtag atactatact ttctaggctt
     7441 tttgcatctt cactttgtcc taaatttttc tgtacataat ctaagtcaac aatatctacc
     7501 tttccatctc tatttaaatc aaattctctt ttatttgaat ctatattctc taaaacctta
     7561 ttataatcac tcttatctac tttaccgtct ccatttacat cacccattaa gaataattca
     7621 taatttaaag gtttattttt attatctaca cttgctctat tgcttaataa gactctttgt
     7681 gaataacttg atatatcaat gtctttaatt tctttgttct taaatccagc tccagagact
     7741 tctacattgt attttccaac aggtaaatta ttaaatacaa tattataata tgatatattc
     7801 tcatctttat tttttatttc acttctactt gcagataatt tttttatagt atattctata
     7861 ttatctgtcc caacatttac tgttcctgaa tctttttctc caagctttat tattccacta
     7921 ttgttactat catcttttaa tcgtaactgc atattagtca tactttctga ctcgctattt
     7981 tttattggca gtgaaaaatt taaatctact tcaagactcc cccttaactt ttctgtaact
     8041 gatttttcaa ctgcccttag cttcccctct aatataggtt ctgcaaaaac gtttagtggt
     8101 gcactacaca tagatactgc tagtacacaa gatattgcct ttttcatgaa ataacaatcc
     8161 tcccttaata aatatctaat ttttcatgaa tttaaatact ataagtttca gacaataaaa
     8221 aaagacactc aaagagaatc ttaattataa aataaaaaga ctttactctt tttagcaact
     8281 ggctgatatg gctttaaaag ctttaatccc tatagttttg cgtcattaga tttccctaat
     8341 tttgccattt aattttcact actttcttac ccattttata ctatatttaa actttttaat
     8401 acaatttttt tagattaaat tataaaaaaa taacatttat attaatttat taatataaat
     8461 gttattttcc attataattt aattatttat atagcttcct tacaagcata attttgtgta
     8521 tattttctta ttctttatat cttccataaa attcatgtgt agcatataga ttcttatctc
     8581 ctgttatagc tataccaaat cccattattt catacccttt atgcaacata ttagttcctt
     8641 cttctggatt tgtcttccac tcattaaaaa taaagtttcc tatatcatct gcatctctat
     8701 ttgtatatac aggtttatct ggaattttat atttaattat attttcacct tgaactgcta
     8761 tatatccagg tttaatctca ctccaatcta agtatttcaa aaaagtagaa tatgaatttt
     8821 ttccatttat cgtatgagat aaagtcttct tatttgccat taaattagac caatcatttg
     8881 ctagaccctc taatctgctt gctacagata acgctcttac tccattctct tttctatact
     8941 gctttgtaag agcatatact ttacccttta ttctctcttt aaatgctcta tcatttggat
     9001 tgtaatataa accttgttga tttggtgaag cttgatttat acattgcttg ataacattct
     9061 gatttatgcc tcctagtaca gtcaatttgt ctgcaccact taaaatactc ttgttactgc
     9121 cattttcaac taaaactaca ggagcatttt ttgctattgt agaggcagcc aaagcgtctg
     9181 ttaattgtaa gcctttactt agataaaatt ctttactatt tttataaaac tcttgtataa
     9241 ctatcttatt agtttcaaat ctatctgtgc cacccaatct aatagaattt gtgctcttaa
     9301 ctaattcgtc gctgattatt tcactagaac caagcgcata actctttaag cctgtcgtgt
     9361 caaatcccac actatttcca tctgtaagta ttataggtac tccatctcta gcagctactg
     9421 gtgatgcact tatcgcatct gcttctcctg taaatccatt ggctataatt actttatcta
     9481 ttggcttaat ttctttgatt ttttcagcta tttttatact tgtatcaaat ctattttcac
     9541 caaatattct ttgtacaacc ttctttttgg atagtaactg attctcaaca tttttactta
     9601 ttgttgattc tgagcctatt atatacacag tctttgcttt gtttaatctt tccatagttg
     9661 tgtttggaat ctggttttgt tttgtcatca aaataggcgc atttaaggct ccagctaaac
     9721 cacttgcact tagaccatct gctaagctat tatctgagtt gattagtatt gctgtattgt
     9781 agttttgctt atctgctatt tttgctgctg tctcatatct gtcattccct tttatttgct
     9841 caactgatgt caacgcattt accataggag tatttaccat tactaaaaat agtgtaagtc
     9901 ctatagctaa tatcttttta ctattcttca tattattttc tacccccaat gtacttttca
     9961 tattttttat tagtactcat acttattttt aatcaaaagt tacaattttg ttaccatatt
    10021 ttatatatga taccatagtt atccatacaa ctcaaacaat atacctaaag tttttattct
    10081 ctataaattc aaaacattat aatttatttt atttaattaa gttataagtt caaaataatt
    10141 tatgttagta atatgtttaa tcattataag aagtatcttt ttataataat ttttgcaata
    10201 tgaatttctc aacattataa tcaaaaaata aaataaggta tctcaatcta attctaaatg
    10261 agacacctta tttttattat tttatcttgt tatttgtata ttttataata tctacactat
    10321 gtcttacata ttaacgcatt gtataatcat atattatcta aataaacttt taatattaat
    10381 tcctatatct tattagttct tagtaaataa gtttattttt tctgctattt tttcaatagc
    10441 gtttttagct attccttcac ctatttgtac taacttagta gcattcttaa gtcttaactc
    10501 aactacttct tcttgtgatt tagaaagttc ttctgtagtt aatactattg gagcctttga
    10561 actagctgca agtggtgctg ctgtaagtgc atcaactaat tgtccatttc cacctacata
    10621 accatctttt gctacatatg ctttagttgc acctgcatag tatttattga ttactttagc
    10681 attagtatct tttctatttt ctccagctaa tctttcaact ttattattta aatctatatc
    10741 aactaatttg tcttcaactt cttttgatac agaattttct ccacccacta tttctaattc
    10801 ttttcctttt aataattttt cagcatttgc atctaattcg ttacctgtaa ctattattgg
    10861 agcaccaaat tgagcagctc tagctgatat actcatagca tcagcttctc catttccacc
    10921 tactacaaaa gcagtcttaa atgtatcttt taattgtttt gctatttcta aagaagtttc
    10981 aaatctatct tctccagaaa ctctctcaac tttacttaaa cctaatttag aaagtttttc
    11041 ttcagtttct tttgatactt tagattctcc acctactata tatattttct tagaagatag
    11101 attacttcca agtactctta atatttcagc ttctatttct gatggtagtg aatctttttt
    11161 agataataat attggtgcat ttttttgaga ggcaagtggt gctgatgcta atccatcaac
    11221 tatagcatct tctccaacta aaattatagc ttctgcttct ccatctttga atctctcttt
    11281 actaacttct actgctgttg taaatcttga atcaccagat aaaacttcta cttttaattc
    11341 ttttccacct tttacatcag ttaatacttt gtgtaagtct ctaactaact tatggttttt
    11401 acttgaaaca cttattgttt ctggcttttc atttgccttg cttaaaacta tatcaaagct
    11461 tgatttttct ggtttatctt tatcatcact atcttcttgt tcttgagtaa ggattgcttc
    11521 attttcaaat gttaaagcat atccattttt aactttatta actatttctc ttcctaatgt
    11581 agttaatcta attccatcaa ataattcttc tgctgatatg tcttgtaaat ctgcgttttt
    11641 aactgttatc gtatgtaatt cttttgaatc tatgtcacta taactctttt cataaccttc
    11701 aaaagtacct tcttctgatg ttatcacttt tgagaaatct aaatgttggt ctcctacatt
    11761 taatgtaata acatttgctg aatcttttgc tctttttact tcaactgtat ttgtagattt
    11821 tatagttgct gttaacttgt tatcagaatc ttctgctaaa gttgcattgt aagcattaac
    11881 tgcatctact atttcttgag cagttttgta gttttcaatt ttatagtcaa ctacttttcc
    11941 acctaacact tgatgtcctt tatcttgaat agtaactttt aaactttgcc cttctgttaa
    12001 agagtttatc ttattttgta attgagttgc attagttaag tttacattgt ctacttttat
    12061 atcgtaaact ctatcattta catttgctcc tgcttttgta tcttcaaact taacttctaa
    12121 tgcttttcta acctcttcga ttagtttagc agaatctttc gcacttactg tgtaattttt
    12181 atcaacattt tctgtcacag tattgtctgc gaatattggt gctgctgcac tcataactgt
    12241 agcacctgcc attactattg ccatacttct ctttttgttg cttaacatat ttatgtcctc
    12301 cgaatattat tttttatttt ttatattcgt atttcccaca cataaataat attatatata
    12361 atattatttt tcaatatatt ttatgtgttt acataattta ctaacacaaa cactaataat
    12421 atcttcttga accccttccc cctacttagc tctctataat tatgctcaaa gatagaaatc
    12481 cctattttat agtgattttc tactaaacaa tcatatacta acaaaagtat tcaaactcca
    12541 tttttatata ctactatgca ctaatttttg ctttactctt attacttaaa tatat
//
LOCUS       DAKBNN010000113        12566 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000113 DAKBNN010000000
VERSION     DAKBNN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.114"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(118..978)
                     /locus_tag="OUB02_002524"
     CDS             complement(118..978)
                     /locus_tag="OUB02_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT8886271.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1127..2017
                     /locus_tag="OUB02_002525"
     CDS             1127..2017
                     /locus_tag="OUB02_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8886272.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLIPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPPNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            2235..3989
                     /locus_tag="OUB02_002526"
     CDS             2235..3989
                     /locus_tag="OUB02_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HCT8886273.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKNYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            4373..5359
                     /locus_tag="OUB02_002527"
     CDS             4373..5359
                     /locus_tag="OUB02_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT8886274.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            5528..5676
                     /locus_tag="OUB02_002528"
                     /pseudo
     CDS             5528..5676
                     /locus_tag="OUB02_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            5695..7176
                     /locus_tag="OUB02_002529"
     CDS             5695..7176
                     /locus_tag="OUB02_002529"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCT8886275.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENLYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            7202..8173
                     /locus_tag="OUB02_002530"
     CDS             7202..8173
                     /locus_tag="OUB02_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCT8886276.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(8429..9031)
                     /locus_tag="OUB02_002531"
     CDS             complement(8429..9031)
                     /locus_tag="OUB02_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8886277.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEDSRQFAKQIYYILDNSIEVDNC"
     gene            9312..10523
                     /locus_tag="OUB02_002532"
     CDS             9312..10523
                     /locus_tag="OUB02_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HCT8886278.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNIKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            10591..10788
                     /locus_tag="OUB02_002533"
     CDS             10591..10788
                     /locus_tag="OUB02_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCT8886279.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAIFSPI"
     gene            10822..11793
                     /locus_tag="OUB02_002534"
     CDS             10822..11793
                     /locus_tag="OUB02_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8886280.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            11980..12357
                     /locus_tag="OUB02_002535"
     CDS             11980..12357
                     /locus_tag="OUB02_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8886281.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         5124 a         1318 c         1989 g         4135 t
ORIGIN      
        1 gatacattta atttttatct taaaaacaac aattataata tataaatagc aagtataata
       61 taacatataa aaagttgaat ttgtatatgt taattatact tgctatttat ttgttagtta
      121 ttctattttt ttaaatccat tacctaaagc ttcatgtata tctgtaatta taataaaagc
      181 attagggtcg acatttttta ctaatttttt caattcgaca acttctttct tttcaactac
      241 tactaaaagt acatttttat tttctccagt atatgcacct tctcctttta gaatagttgc
      301 acctctatct aaatcttcca ttattttttt acttatatct tttgaaaggt cagatataat
      361 agtgaatgat ttggaataat taaatccttc aataagcaag tcactaactt ttactaaaat
      421 gtataaagcc acagttgaat aaagtgcaat ttcaacctgt ctatttacta tacctgataa
      481 aacaactaca acgcagtcta caatacccat taaaacaggt atactaaatg taggaaaata
      541 tttatttagt aggagtgcca ttaaatcagt tccacctgta gagccattta ttctaaaaat
      601 aagtccttga ccaactccca ttaaaataga accagatata attgcaagat acatatcatt
      661 tgttatatct aatgtagcca tatttgcagt aagcttaagt gctatggtaa gaaaaattat
      721 tcctaaaacc gttttaaaac aatctttttt tgaaagtatt ttgtaagcaa atatgaataa
      781 tggaacattt aaaagtaaat tgacaatcca aagttctaca ccgaataatg aatttaaaac
      841 aactccaagt cctgttatac caccagcagc gattgcatgt ggattaaaaa ataagttaag
      901 agaaatggcc attaatatac agcctacaat taatattaaa atttctgaaa aagttattct
      961 atcttttttc aataacatca ctccttatat atagattata atatatatta tatagttttt
     1021 ccaattttta ataaaagaaa taaaaatata ttataaaaac aatttgattg gaattttcag
     1081 aaaattaatg ctataattaa aagtaaattt aaagggaggt tataaaatgg ctaaattaac
     1141 tctagaacat ataaaaggaa atacatatta tattccactc cctacaatag taggtgtata
     1201 cgtagatggt aaagatgcaa ttttaattga cagtggaaat aataaggata cagctagaca
     1261 agttttaaga ttattagaag aacataattt aattcctaaa ttaataataa acactcattc
     1321 aaatgcagac catataggag gaaatgcata tttgaagaat caaacaaaat gcaagattgc
     1381 tacgactaaa attgaaggat attttacaga aaatccaata ttagaatcag catttttata
     1441 tggtggctat ccaagtaaag cactgaagaa taaattttta ttagcaaagg aaagtgaagt
     1501 tgattatatt atacctccaa atggcaaaat tgtagataca gaacttgaag cgatttctct
     1561 tccaggtcat tattttgaaa tgattggtgt tagaactcca gataatgttt tgtttgttgc
     1621 agatagtctt acaccagaga atataattac taaatatcat tttttcttcc tactggatat
     1681 agattcacag tttaagacac tagaaaaatt gagaatgtta gaggctgatt tctttgttcc
     1741 aagtcactct gtcaaaacta cagatataaa aagtttgatt aatattaata agaagaagat
     1801 ggaagaaata atagataata ttaaaaaggt ttgttatgag ccagttatga ttgatgaagt
     1861 tattgaaaaa atgtgtgatt tgtacaatgt caagcttgat gctaatcaat atgtgttggt
     1921 tggaagtaca atacgttctt atataactta tttatatgag aataatatgg ttgaatatat
     1981 atttgatggt ggtaagatga tgataaaagc tgtgtaaata gtaataattt tcaaattata
     2041 tgatgaaatt gttttagtat gactgttatt tgaaaagtaa tataaaaaaa tttatataaa
     2101 taagctaaga tgattgatat aaagttactg ataattttaa ggtaatatat acgttactat
     2161 aatattatgt tattatttgt aaataagggg ataatttata agcaaattaa agaccttaat
     2221 ttacaggagg aatagtgtat attatgaata aaaaattcaa aaaattcagt aaaaaactaa
     2281 gtatattaat tttagttttg actatgtttc tttttaataa tttaactgca aatgcacaga
     2341 ttaaagaaga ttcaataata ggaattgata gatatgaaac agcaggactt atagcagata
     2401 gacaaactta tgatactgta atattagtta atggagataa aaatttatct gatgggcttt
     2461 catcaagtgg attagctgga gctattaacg caccaatact tttgactaaa aaaagtgaaa
     2521 taccaaaagc tactttaaat agactagata ataagagttt aaatgttgta aaaaaagtat
     2581 atataattgg tggttataat actattgaaa attcagtaga aaaagatata aaaagaaaag
     2641 gtattcaagt ggaaagaata aatggtaaca atagaattga aactagttat aatgtagcta
     2701 aaaaaataaa tgaagtaagt agtgtaaaag aggtaatgct tacaaatgga tttgtaggag
     2761 aggctgatgc tatgagtata gcacctgtag ttgcaaaaaa taaaggagct ataatactta
     2821 cagatgggaa atcagttcca tttaagactg aaggtttaaa tgtttacgct ataggtggca
     2881 aatctgcgat aaatgaggat ttagtaaatg aaactaaagc aattagaatc ggaggaaatg
     2941 atagatttga gaccaataaa aaggtaatgg aaaagtttta taatggagct acagattttt
     3001 atataacaaa tggatatcaa ttagtagatg cactaacatt atctccttta gccaaagaaa
     3061 aaccaatcgt tttagttgct gatggaagta ataaaggtat tttaaaagga gctaaaagta
     3121 taacaaaagt tggaggtatt gatgccaata tatataaaca gtgtttagat atgattaaat
     3181 ataataatat ggatatgact ccaaatattt taaaacactt aacttcagtt gaaggaaatg
     3241 aagtttctga aatacttata gaatcagata atttaggtgt ggttgttgaa aagacgaata
     3301 ccaataaatt tgagtttgat tacgtaagtg taactaatga aaaaaattgt actttttctg
     3361 tatataaaga aaattcttct aataatataa aaaatggaaa gctagttgta agtgctaaga
     3421 aaaaaataga taagaaagat agtgatttgc aagatatgaa tggcgacaat atgataaatg
     3481 caaataaaga taggatggta aatgtgataa aaattggagt tcccgataaa atgtattcta
     3541 attttaaagt agatgttgaa agtggttcag tagaacttta taatattaaa ggaggagcta
     3601 ctgttgatgt caatgacggg attgctaaca ttgttgataa caatgtaaca aatcctttca
     3661 atataaatac taatagtggg atttcaggtg taacagctga aactatatca tctgaaataa
     3721 aatttagagc aaacaatgga attgtaaatg taacagctac gaatataaat ggcaatataa
     3781 gtttatttgg aagcgataat aaaggtacct ttgatggaat atttaaatta aatctgaaaa
     3841 aagaaccatc aaatttacac ttgaagttaa ttggaaatgg gataaataaa ttgccagaag
     3901 gttggtctaa aaattatatt ttaggaaatg gacaaccaat tattgaagtt aaaaataatg
     3961 gcataaataa tatatcaatt ggagaataaa aacatatgaa aataaagttt atatagtttt
     4021 aaaaattata gaattttaaa atatttatat atattcttta aataaaaaat tattttttaa
     4081 gtgataatta agtatttatc aataaggcta tggttgtaaa aatcatagcc ttatttttat
     4141 attataaata aattagagta tttatcaatt catagtagat aaaattgaaa ttaaatataa
     4201 caacaaaaaa tgaaaattac aattaattta atgttaaaga ctcaattatt tggatgtgga
     4261 atggatagta caagtagtat ataaaaagta ttgactggac aaataactat attttatttt
     4321 aaatatataa tataatgtaa taaaatatat caaatgtaga aggtgataac ctatgaaaaa
     4381 aataacaata aatgatattg ctaacttggc aggagtatct aaaagtactg tatcaagata
     4441 tctaaacaat aaagatatca gtgattctac taaagagaaa ataaagacaa taatagatga
     4501 atatggatat gagccgaatg catttgctca aagtttgaga gctaaaaaaa catactttat
     4561 tggtattata acaccgtgtt tagattcttt tgtaaaatct aaaattatga tggcaataga
     4621 tgaagaactc aaagaattaa aatatacatc actaattatt aatactaata gaaaaataag
     4681 gtcagaaata gatagtatat caaagttagc tagtctaaaa gttgatggaa taatactaat
     4741 tggtaccgaa ataactaaag aacataagaa tgaaattgaa aagttagata taccgatagt
     4801 tgtagttgga caaaaagttg atggtatcaa tagcatagtg aatgatgatt atggagcagg
     4861 atataaaatg ggtcaatata ttgctaataa aggttacaaa aacattgtat atttaggtgt
     4921 tgatgaaagt gatatatcag ttggtttaaa tagaaaaaat ggagtactta atgggttaaa
     4981 agataaaggg tatgatgcaa aagtatttta tactgacttt gaccaagaaa cgtctataca
     5041 aagaagtgga gaaatgctag aaagtgaaaa tccagatata ataatatgtg caactgataa
     5101 tatagcaata gcaacaatga aagaaatcaa taagagaggt aaaagtatac cacaagatat
     5161 atctgtagct ggatttgggg gatatgatgt attgtctatt ataagcccta aacttacaac
     5221 aataaagttt gaaaataaaa atgcaggtaa agtagcagca aatacgatag taaacttaat
     5281 acaagaaaga aaagaaccac tattaaagga gattaaattt gaattaatag aaggtgaaag
     5341 tacaataaat aaaaattaag atacacatta catatattag taatgtgtat ttttttacta
     5401 aaaaaactat aattctaaaa attaggaaaa cttttttgaa agaaatattg catctgaaat
     5461 aaatttatgc taatctttaa ttaaaccggt ttcgaaaccg gtttaattaa accgatagaa
     5521 tagcaaaatg agtgcaaaaa taactgattg taacaaagta tcaaagtaat tattggagta
     5581 cataggaata aacaaaatat aaaagatgta gcacattgtg taataagact aagaattgtt
     5641 ttagatagca ataataaagc aaatataaaa ttataaaaga aaaggaataa aaatatgtac
     5701 aaagagccta aatatagaac catattagaa tcgacaaaag aagaattaaa taaattaaga
     5761 aatatatctt taaatgataa atataaacca ctatttcata tacatccaca acatggacta
     5821 cttaatgacc caaatggatt agcatattat aatggtaagt atcatgtatt ttatcaatgg
     5881 tatccatatg atgcaactca tggaatgaag cattgggctt atgtaagttc tgatgatttt
     5941 gtaaactgga atagagaaga tgtagcatta ataccaatcg aaagttatga atctcatgga
     6001 gcatattcag gtaatgcgat agaggtagat ggaaaattgc atatgtatta tacagggaat
     6061 ataaaatata gtgcagaaga tagatatgca tatcaaaact tagctataat gaacaaggat
     6121 ggtaaaataa ctaaatatga aaataatcca atagtaagtg aaataccaaa aggatataca
     6181 ggacatgtaa gagacccaaa agtatttaaa agaaaggata aatactttat gttacttgga
     6241 gcacaaacaa gtgataaaaa aggagttata atagtatatg aatctaaaaa ctctatagat
     6301 tggaatttta aaggagaatt aaatgtaaaa aacatagatg aagattttgg ttacatgtgg
     6361 gaatgtccag attatattaa tatagatgaa aaagatatat taatattttc accacaagga
     6421 gtagaaccta aaggatttga ttatcaaaat atatataatg tagtatatgc tattggtaat
     6481 atggatttag ataatttaac atttgaaata gatacaatga aagaactgga aaaaggattt
     6541 gatttctatg ctccacaaac atttataaag gactcacaaa taatactatt tgcatgggct
     6601 ggaatgggag aagttttata ccctacagat aaaaacaaat gggcacattg tctaactgtt
     6661 ccaagaaaat taaatataaa aaataataag ctattgcaaa tgcctgtaga tgaattaatt
     6721 aaattaagat atgatgaaac aagtgggcaa aatactataa agaataatat caatattata
     6781 gaaaataatg aaaatttata tgagcttaat attaatataa aaaatattga cagcaataaa
     6841 tttgggctgg aattattttc atcacaagat gaaggtgtaa aattagaatt taacaaatta
     6901 ggaaacattg taactttaga cagaagcaac tttaaaaaag ttttcggtgt tgaatatgga
     6961 acaaatagaa aagaatatat aaatattgat gaaaatacta atatcaaagt actagcggat
     7021 agaagtattt tagaaatttt tataaatgat ggagaagtag tatttactag tagaatattt
     7081 gctaaagaaa attctaatca aataagggtt tatagtgata aaatagtaga gtatgagtat
     7141 actaaattta aattaaagca aggtattgaa ttataaaaac acattataag gggtagaaaa
     7201 gatgaaaaaa gtaattagca taggagaagc tttaatagac tttataccga atcaaaatgg
     7261 tggtaaatta aaaaatgtat cagaatttag aagagtagca ggaggagctc cagcaaatgt
     7321 tagtgctgtt gtagcaaagc ttggaggaaa atcgagtttt atatctaagt taggaaaaga
     7381 tgcatttggt gattatataa tagatgtatt aaatgaagta aatgtaaata ctgattatgt
     7441 attgagaaca agtaaagcaa atacaggact tgcttttgta tcattaaaag aagatggtaa
     7501 tcgtgatttc tcattttata gaaatccaag tgcagatatg cttttagaag ctgatgaagt
     7561 taaaaaagag tggtttaata attgtcatat cctacatttt tgttcagtag atttaataga
     7621 tagtccaatg aaattagcac ataaaaaagc aatagaatac gcacttgaaa gtaatagtat
     7681 aataagcttt gaccctaata taagattacc tttatgggat agtgagcaat cttgtaaaaa
     7741 ggcaatatca gaatttttac cttttgctca tattgttaag atatctgatg aagaattaga
     7801 atttataaca ggagaaaatg atatagaaaa atcattacat aaactctttg tggggaatgt
     7861 tgaactagta ttatatacaa aaggaaaaga tggagtagat gcatacacta aaaaagtaaa
     7921 aggtatgtgt aaaggggtta aagtaaatgc aatagataca accggagcag gagattctta
     7981 tataggttca tttttataca ccttattgta taaacaaata acattgaatg atataaaaga
     8041 aatgaaacaa gaaacgttaa atgagtattt agaattttct aattattatg cagctcgtag
     8101 tacaactatt aaaggtgcga taagttcata tgcaacaaag gaagaaataa ctgaatttat
     8161 aaaaaattta tagtaggaat gagtaatagg ttttgaagga agtaacatgt aaagagtttt
     8221 taaggatgtt tactatgaaa ataattaaca tataaaaagc aataaagagg ctgtctcaaa
     8281 atagagataa gcctctttat cattgttgaa atgataaaaa atattttaat tattgttact
     8341 ttttagaaga atatatatct tattttaaat aagcaaatta tgtaagattt atttatatta
     8401 tcaatacttt acttggagta ttatgtcttt agcagttatc tacttcaatt gaattatcta
     8461 aaatataata aatttgtttt gcaaattgac gagaatcttc ttctttttct ttattcataa
     8521 aattaggttt acctaaatca cgttcaacaa gcattcctat atattttaac tgtgaatgct
     8581 tacagtagcg cttcatacca ttttcccaaa tatcagcacc cttttcaggt ctatatccac
     8641 atgttgtaat aagtgctact ggttttcctg cccatagagc ggggcctttc tcattaccat
     8701 agtatttatt cattccatac accaatctgt caagtagtgc tttcattgga gaagtgcagt
     8761 accaagaata aattggagta gcaaaaacaa ttaaatcaca cataagaatt ttcttgaaaa
     8821 ttttctgaac atcatcttta caataacatc caaaatcaaa taagttatcc tgacactttt
     8881 tacaagcaat acatggctct attttacaat catataacca tattacttca atatttgaat
     8941 tataattttc taattcctca ataaaaggtt ttaaaatagc agcagtatta ccttttttct
     9001 taggacttcc cataagaatg catacttcca tattgatacc tccacagtat tttaaaatag
     9061 tttttagtgc ttaatatagt tttaggatac tatgagaata cgttaacaac aatacacttt
     9121 gtatatttgt aataaaattg aaacaaattg ttttatcaat aaaagttgga atgtatctat
     9181 aattaaaaat attttaggta tgtttttaca tatatgtgtg tagttaacta tggtaattta
     9241 ttgtaaaatt atcttatagg attataattg cttaatttat gattatattt aaaatcttgg
     9301 aggtaaaaaa tatgtactca aatgaagact ataaatttat agaagaaaat gcaccatgtt
     9361 atatatatga tgagactaat ataattgaaa aatgtaagga attgaaaaat aatattttag
     9421 gttttgattt tttatattct ataaaaacaa atccatataa caaaattgta aaaagcattg
     9481 cagagcaagg ttttggagca gatgcttctt caactaatga agttatgatt tcatcaaaat
     9541 gtggagtttc taaagatgat atttattatt catcaccagg caaaacagag attgatatag
     9601 aaactgtttt agataaatgc ataattattg cagatagtat tacagaaata gaacttataa
     9661 ataagatatc taaaaagcat aatcagaaag ttaaaatagg aataagaata aatcctgatt
     9721 ttactatgga tgatgatagt gctaaatcaa gcaaatttgg tatagatata gaagatatga
     9781 gcagtgtttt taagatgtta agtatgtgtg attatgtatc tgtagttggt attcatatac
     9841 atattaaatc tcaggtattg gattttgaaa agttaggaca ttattatgaa aaatgttttt
     9901 ttatagcttt aaatttaaat aaaagtaagt atattaatat tgaatttata aattttggta
     9961 gtggacttgg agtagcttat gataatctta gagaaaatcc acttaatctt aaagagcttt
    10021 caaaattaac tcaaaagatt gcttttacta attcaaacac actaaaatca agacttctta
    10081 ttgaatcagg acgtttcata gtttgtaaat caggtaatta ttatgttaaa attgtagata
    10141 ttaaagtatc aagagggaaa aaatatctta ttgtaaaaaa tggacttaat ggatttatga
    10201 gacctattat ttctaattta attgaaaata ttacttgtgg agtagaatta gaaggtcaag
    10261 agccattata tacatcaaat catgcctttt ctctttcagt attaaataat ttaaaagaag
    10321 aagagtatgt aaatgttgtt gggaatctct gtacatcatt agatgtaatt gcaaataata
    10381 ttaaattgaa aaaagcaaac ataggagatt tgatttcaat tagtaatgct ggaagttact
    10441 cctattcttt atcaccacta ttattttcga gttatgaatt tcctaaacaa tattataaaa
    10501 gaaaagatgg atatattgag taaacaatag cataaattaa taaacttagt attattgaaa
    10561 aaatttaaaa tgcagggggt gtaattctat atggaattgt ggaaaagaaa tttatttgta
    10621 tgttggatag gtatgttttt tagttctata gggatgagcc aaatagcacc tatacttcca
    10681 ttatatatta aacagttggg ggtaactgat gttagtttaa tacaacagta ttcaggtata
    10741 atttttggat gtacctttgt agtagcagca atcttttcgc caatttgagg gaaagctgct
    10801 gataaatatg gaagaaaatc aatgctttta cgtgcaagta ttgggatggg aatagttata
    10861 tttaccatgg cgtttgttca aaatgtctat cagttattag gattaagaat acttcaagga
    10921 gtttttacgg gttatgctac tgcatgtact actcttattg caacacagac agataaaaat
    10981 cattcaggat gggctttggg tactcttgca actgcatcaa ctactggctc attaattggt
    11041 ccaacagtag gaggatatat agaatcaatc ttaggtctta aaagtacatt tattataaca
    11101 ggaggattgt tgtttgtatc atttatcatc tctattttat ttgtagtaga taattttaaa
    11161 cctaaagaaa taaaagaatc aatttctata cctaaagaac aattaaatat attgccaaat
    11221 aaatttttaa ttgcatctat ttttgtaacc acattcatta ctcaactagc tttatactct
    11281 atagaaccaa ttgtaaccat atatatatca caacttacaa attttgcatc aaatgtagct
    11341 ttaatagctg gattgacatt ttctgcatca ggattagcaa atttattagc agctcaaaaa
    11401 cttggaaaaa tgtcagataa aataggccct caaaaagtat tgttaatttc acttttatgg
    11461 gctgggatta tatttatacc acaagctttt gtaaaaactg catggcaact gatgttgttg
    11521 agatttttat tagggttgtc agttgcaggg cttaatcctt ctgttaacag cttgcttaaa
    11581 aaaattgcac cagaagaata tgtgggaaaa atatttggat ataatgcatc tgcccagtat
    11641 ataggctgtt catcaggagc atttttaggg ggacaaatat cagcacattt aggtattaga
    11701 acagtatttt tttcaacaag tttattgttg tttatgaatg cactttggat gtataaattt
    11761 actggattgt ttattaaaga taagactaaa taatcatact atttacattt ctaattttat
    11821 gatgtaaaat aaatattttt tatgtaattt atcataaaat tatataaaaa ataaccctat
    11881 ggtaaaaatt ggaaaatatt attatcgaca aaatctttga cttaaaaact atattgcgat
    11941 agtattaatt taataaaagt ttgagagggg cgtaaggtta tgacaatttc aaaaatacca
    12001 caagcagaat taaaagttat gaagtttatt tgggaaaaaa atgatacagt aacatcaaaa
    12061 gaaactattg aagctatgga acgaaaatat ggttggaaac aaacaactac attgacactt
    12121 ttatcaagac ttgttaaaaa acatttttta agtgcagaaa aaatagatag atacactcat
    12181 tatacaatag ttgttggaca taaggagtat ttaagtgttg agacaaaaga ttttttaagc
    12241 aatatacacg acaattcttt acagagttta atttcagcac tacatgatga tgaagttgtt
    12301 gataaagata tgttggattt atttgaaagt cattttaaaa atttagaaga agagtaatta
    12361 tatcaggata gaactctaag aatttaatta atctatggct gaaaaccata aatggtatta
    12421 tttaatcagc tactcactat tcataaagta atattaaata ggttctctaa ttagtaaaat
    12481 gcctaaaata attggaatag ggagtatcaa gttaaatgat accacttatt taaataagaa
    12541 atgattttta aaatttaaaa taattt
//
LOCUS       DAKBNN010000114        12463 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000114 DAKBNN010000000
VERSION     DAKBNN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.115"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(105..974)
                     /locus_tag="OUB02_002536"
     CDS             complement(105..974)
                     /locus_tag="OUB02_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8886282.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1071..1928)
                     /locus_tag="OUB02_002537"
     CDS             complement(1071..1928)
                     /locus_tag="OUB02_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8886283.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2143..4161)
                     /locus_tag="OUB02_002538"
     CDS             complement(2143..4161)
                     /locus_tag="OUB02_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCT8886284.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMLAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4432..5208)
                     /locus_tag="OUB02_002539"
     CDS             complement(4432..5208)
                     /locus_tag="OUB02_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCT8886285.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5265..6236)
                     /gene="asrC"
                     /locus_tag="OUB02_002540"
     CDS             complement(5265..6236)
                     /gene="asrC"
                     /locus_tag="OUB02_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HCT8886286.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEDINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKKPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6249..7040)
                     /gene="asrB"
                     /locus_tag="OUB02_002541"
     CDS             complement(6249..7040)
                     /gene="asrB"
                     /locus_tag="OUB02_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HCT8886287.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7041..8048)
                     /gene="asrA"
                     /locus_tag="OUB02_002542"
     CDS             complement(7041..8048)
                     /gene="asrA"
                     /locus_tag="OUB02_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HCT8886288.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDLKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(8439..9128)
                     /locus_tag="OUB02_002543"
     CDS             complement(8439..9128)
                     /locus_tag="OUB02_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT8886289.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLELMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     EIELSRYFKNN"
     gene            complement(9191..10039)
                     /locus_tag="OUB02_002544"
     CDS             complement(9191..10039)
                     /locus_tag="OUB02_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8886290.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(10058..10945)
                     /locus_tag="OUB02_002545"
     CDS             complement(10058..10945)
                     /locus_tag="OUB02_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT8886291.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(11003..11806)
                     /locus_tag="OUB02_002546"
     CDS             complement(11003..11806)
                     /locus_tag="OUB02_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886292.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(11966..12418)
                     /locus_tag="OUB02_002547"
     CDS             complement(11966..12418)
                     /locus_tag="OUB02_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCT8886293.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
BASE COUNT         4008 a         2251 c         1104 g         5100 t
ORIGIN      
        1 aaaatcatgc tacatcaata gtaagcaact attaatgtag catgattttt tgtatctaaa
       61 taatcttagt atttacctat caatatagat tgatatatca aaaactatag tcttctatat
      121 tcttctcttt ttagcttttg gttacttaga tagtaatcaa tttcatcaat taaacgttgt
      181 ttatcatcag gtaatacacc ttttaaatta gcataatttg atgcatgatt acttctaaat
      241 acaatttttt cattagcatc tatattcttt atcatctctt taatttcttg taacacagcc
      301 ttatcctcta gtattgtaaa ctctcctgcc ttaagtaaat cgtaaagttc agtattaggt
      361 tctaccatta aactaagtac acccacataa tctggtgaaa ttgctcctaa catttttcct
      421 gtatcaactg catgctgatg tgttttttta gttcctccaa gacctgctat tacagtggct
      481 gataatttaa tcccactttt tttaactttt ctaccaactt ttattaattg ttcactacta
      541 aaacctttct taatatcttc taatacttca ttgcttccac tttcaagccc tagataaatc
      601 attttaacac caagttttct taattcttta agttcatcat cagatttttt ctctatagat
      661 ttaggagagc cgtacatagt aattctttca cactctggaa aaacttcttt tatatataaa
      721 ataatatctc ttaatttctc cattggtatt atcaatgcat ctccatctgc taagaatatt
      781 ctttctacat gcttataata tcttctaaat atatcaattt cattttttat tacttctagt
      841 ggctttattt taaatttttt agatttgtac atactacaaa acgtacattt gttatgagaa
      901 catcctagtg tgacttgtat tataagacta taagcctcac ttggtggtct gtaaagtggc
      961 atatcgtatt ccatattaaa atcccccttg taataaaata ttcattctat tatattatta
     1021 atatttactt taaataataa cttataaaat tatatttttt aaattttagt ttaagatata
     1081 ttctttaaaa atttaatgat actattggca gtaatattaa gttgtttttc ttgacttata
     1141 atagcatcat tatctccctt ttgttttcca taatctccaa attgtgcatg atttcctcct
     1201 tcaatctcaa cataagttgt atcatcagga agtttttgtt ttgattctac aagactttta
     1261 aaattgacta ctccatcttt gctcccccat atagatataa ctttttttcc taattctttc
     1321 aactcatcac ccattggata agaagctaaa aatacaacgc cttcaattaa tttattatca
     1381 cgtgcaaaat ttgatgctgc aactcctcct aatgaatgac ctcctattac ccaatgttct
     1441 atgttgttgt aagaatccat aactttctgt gccttattag ttccaagtat agctagatta
     1501 agtggcatct ttacgattac aacctcatat ccactttctg ctatttttct tgcaagtgga
     1561 gcataagatt ctatttctac tttagctcct ggatacaaaa ttaaaccttt agaaactttt
     1621 cttccttttg gtgtaaatga aatgttttca tttacagtta cttcaacttt actattgctg
     1681 actagagctt cttttgccag cttttgaggt tcataggttt tatttatcca tatgaaaaag
     1741 ccaagcaaaa tcaatacaaa tagtatacct attcccttaa atatcttatt ctttatgtta
     1801 aattttctat tattattttt atcatctttg ttctcaacct catcataaag taaaggtctc
     1861 tcaagttcca tagttatact aaatgtatca tatttcttat tacgctttac ctttacatct
     1921 ttactcataa atctaacccc cataatattt gaatactatt atagtttatc ctaagtaaat
     1981 tgttttttca accaatgaaa aaaatcaata ttttaactat tttcatataa aattcataaa
     2041 tatatgtttt ttgtttttta tatattattt tcttttagta ttttataaaa caaatagcca
     2101 tatactttat tttaaatata tggctattct ataatttaat atctaagctg cttcttttat
     2161 tgaaacttta gttatattac ttattctata catttctttg acaccattta gagtttgcag
     2221 taagtttaga catattatct tgtcacctag acttttagta tttacaatac tctctaaaat
     2281 tacacttctt ccttcatcat acttatctac attgtaattt gaaatataat tagtatctct
     2341 caatattttt tcaacatctt catcttttat tttattttta ttaacattta tatatagatt
     2401 atacatatta cttattagta atttctgatt ttctaaaaaa tcctcttgat gtttatcttt
     2461 atatatagca tttatcaaca taagtctatc ttcaataagt gatggtatca ataataaatt
     2521 ttttatatag taaacatcag ctacattttc tatagactta ttgacttcat caattatatt
     2581 tttaactatt cctttataca tttctataac atgttgatag cctgttttag catcatatgg
     2641 taatataaac ttatttacta ttaatgcaat aattgctcct aatgctacat atgaaatcct
     2701 gcttatggtt aaaacatttg ggtctcccat aactactgct gaaccaagag ctgaaacagt
     2761 tacacatacc attaattttc tataatcaac cacataagag tttatatacc ctgctaaaag
     2821 aactattaac gaccttaatg tactatcttt tattattgaa aataatacaa taaatattac
     2881 agctcctata aatgtacctt ctattctttt tctagacctt tgtatacaat tttctgcgta
     2941 aggttgtgtt aatgaaaaga cagttaacat tatccatctt ccatctctta aatggaaata
     3001 atccataata aaacccgcta cagctgtagc taaagcaatc tttactgcat aagaaaatct
     3061 tatagaattt aaattaaagt ttttcttata tattgatagt cgtttaaatt catgaggtat
     3121 ctttgatttt ctttcagctt tcttttcgtc tcttgatttt acttttttat aatctactaa
     3181 aaactcatat aaattttcta aaacactata aatttctttt aaatttatat ttttagtatc
     3241 ctgagcaata acataatctc taaatcctaa tatctcttta tcagttatgg tatctttatt
     3301 ttctaaacac atcttaatat agtttatttc tttcgttgct acaactaaaa catctgattt
     3361 actatttatg ctactttctt ctaaaatatt ttttatatct tcatcttcaa attctttact
     3421 atcctttttg tatctttcca ggattaaact tattctttct aatgaaaata aaatattcat
     3481 tatgtctata cctctaatac tgatataaaa gtcatctttt ctattatcaa aaataaccat
     3541 tttcaaattt ttagatatag aatttattct ataattacag ctatctattt ttctcatact
     3601 agatttaatt atagaactat catcattaat agtatttttt aaaagactaa ttttttctaa
     3661 taattcatca catatagata tcagttcttt atctccgaca tttttcatcc tatttctatt
     3721 cataacaaat tgagcaagca ttattataac tgctccaaca gctaaagccg acaatctcat
     3781 aggaaattca ctttttccta caggtatact taccataaat atatacatta gaccaaaagg
     3841 tacatatact gctcttttta agtcataact aaatacatac cctattataa ataatgctat
     3901 aaaatttaat aaaactccta aatacatatt ttgtactgca aaaaatgata gtattcctaa
     3961 gattatattt attgctaaat acttcagtaa atttttaatt ggagaaatag ttaaatctct
     4021 ttccaagagt gttagcatag ctgttactat acctacacct ataagtgtat ttttacttcc
     4081 aaataaatat ataaatatag aaataaatgc tattataaaa ataaacaact ttgtttttga
     4141 tattactagt ttctttgtca tcatatcccc cctaaaaatt tcaacaagac caattttatc
     4201 atatttgtta tcttattcaa aatgcgcttt tttacggtag gactttaact ttttttatat
     4261 ttagaactat ataattcata tataaacgta ttttttatat atataaccga tatattttcg
     4321 tatgctccca tatttttttt gtttcaaata ttttttcaaa aatataaaaa taactctctc
     4381 aataatacta aaaatgcatt tatcaagaga gttataattt atacaataaa tttaattaag
     4441 cttttcttta cctaatatat aagttccaag acccataaat aaagctcctc caacaatatt
     4501 tccaagtgtt acagctacta aattataaat agctcctcca atagtaactg tacttattgt
     4561 tggtgaaaat aatgaaacac tataaattgt catatttgct acactatgct caaagcctga
     4621 tgttataaaa gcaaataaac ataagaatat cataattatt tttgcagtat cactattagt
     4681 tctaaacgaa cacaacactg atacacatac tagaatatta cataaaatcc ctctaaagaa
     4741 aagagctgta aatggcatgg atgctttact agctgctgta tttgcaaaaa attcagctac
     4801 aggtcctttg tctactagac cagttcctac aaatataatg cctaaaacca aagctccgat
     4861 taaattacct acccaactgt atgcccatat tttagaagta tcttttatac ttacaccttt
     4921 atttaacata ccagcagaca taaccatatt gtttccagta aataactctg ttcccgtcat
     4981 tataaccaaa cttagtgcta ttgcaaatga taaccccatt actatcttag tcataggtga
     5041 acctgcatca gtcaaaagtc ctccaatagt aaatatcaaa agtataccta tacctacata
     5101 aagtcctgca aacgcagaag ataccaaata ttttacttta cttgtgttta ataaatttat
     5161 cttatttata gctgcattag ttaatttatc caaagtttct ttatgcataa ttccctcttc
     5221 cttctattaa attttaattt attcaaatat atgttttact caatttatag atatttaatt
     5281 ccactccagt atatgttttc gtacatttta gttatttctg gaagctctac accttcaagt
     5341 gcccattttt taaattccat aaatcctgtt ctatcaatta tatatcctat atgttctttt
     5401 cctcctggtg catctttatc tatatacttt tctacatatc tataagtatt aagtattatc
     5461 tttattatgc tgtcctcatc tacccataaa agaaaatctt ctgctagtct aggttttttc
     5521 tttcctgttc tacccattat agctaatcta tagtattttt tctcatctct agtccatgca
     5581 tttgttgggc aatttagtac acactctcca cagcctatac acttatcttt atctcttacc
     5641 accttattat tttctgtttt taatgctcca acagatatct tagaacattt ttttacacat
     5701 gctccacaat ttacacatct atccttttca tatattggta aagccatacc aataattcca
     5761 aagtcattca ttctagcttt tatacaatca tttggacaac cagttaaagc cactttaaaa
     5821 tgtaaatcat ttggaaatat agctttttct attcttttag caaattcagt tgtattatat
     5881 tgagcctttg gacagacttt atttcctata caagctgcta tatttcttgt acctgccgct
     5941 ggataacctg cgtttttctc actttgattt atattcattt tttcaataac aggttgtata
     6001 attttattta tatcttccat atcttccata tttattccta atatttcaaa cccctgtctt
     6061 gtagttatgt gcacctgtcc attgccgtac tcacttgcaa ttttagaaac tatagctaaa
     6121 ctttcagggt caataagacc accaggtact ctaactctta aagaagtctc atattttgtt
     6181 tttgtaattc tataagcatt tttcattact tttttggtat ttaaatctct tatcacaaac
     6241 ttcaccccct aatcaataag atttttagca tatgaataat caaatactgg tccatctaaa
     6301 catatgtaag tatcatccat tttacaatgt ccacatttac ctattccaca acacattttt
     6361 ctttcataag aaacccaaat gttcttttca tctaaatctc tttttaaaaa ttcttctact
     6421 gtaaatttca tcatcattgg aggtccaact acaactatag aagtattgtt tatatctttc
     6481 atatccaatt ctggtatgta ttttgttact aatcctacat taccactata tccttcctct
     6541 gctccatcta cagtaactaa aatatcaagt tttttactcc atctctttaa atcatctgca
     6601 aataaaatat cttttggtga tttaaatcct actattaatt taaacttttc gcatttttct
     6661 ttattattat aaaaatattc aactattcct cttacagggg ctaatgcact tcctcctgcc
     6721 actactacaa tttctcttcc ttcataaagg gatacatcaa atccatttcc ataaggacct
     6781 cttataaaaa aagaatctcc ttctttataa ttaaacaact cgctagttac ttttcctaca
     6841 tttcttattg taaagtcaat ataatcctct ccatatcctg atactgatat tggactttca
     6901 ccgtatttag gtatagatat ctcataaaac tttccaggca atacaccttt tgtattgcag
     6961 tttactctaa aagtccattc tttatcagta tgtttaacta tatctagtat ttttgcactt
     7021 actggtatat atgggttcat ttatacttcc tccttactaa ttactaaatc atttattttt
     7081 tctatacact ttgaaaatga tatgtattgc ggacaggcat catcacatct tccacagcca
     7141 acacacatat gatatccgaa tcttttttta aaatcatgta ttttatgcat agttttaaat
     7201 ctcattcttt cgccttgttt ttttctaaaa gaatttccac ctgctatatc tgtatatcca
     7261 tctacttgac aagaagccca aacccttctt ctctcaccta cattttcgtt ttccttatag
     7321 aaaatatcct gcattgtaaa acatgaacat gttggacata caaaattaca tctaccacat
     7381 cctatacatc ttgtatcata ttcatcccat attgaatttc ctatcatttc atttgaatca
     7441 atattctctg gcacatctac agatattaaa ttatcagtta cataatcaac attaaattca
     7501 gttgcttctc cattaaatac ttcaaattca ctatccttta aatctaagta cagtgtatct
     7561 ccttctatat ttaatccaat tgaataatta tcagttttgt tagaatccat gctcacacaa
     7621 aaacagtttc taaaactttc cttacaccct acaagtgcaa actttacttt atctctgata
     7681 tttttataaa aataatcttt ttcttcacca ttatttagat atatttcatc aagtcttttt
     7741 ataccattta tgtcacaagc tcttaagaaa attaaaattt tcttgttgtc taaatcgctt
     7801 tccttaaact ctttttcagt aaaataaaac aatacttgat ttatgggaag tacaacttct
     7861 tttggtgaaa agtttgcttt cttagcaaat tccatttctt caaaagcgtt tacctccttg
     7921 tatcttataa catcagtatc tgaaaaagta cctttaaatg ggattaaaac tggtgcaaat
     7981 atcttgtagt ctttttttag ataattcaga ccttcattga acttagatga atctaatttt
     8041 agtttcattt ttatcacctt tcttatattt tagttttact tacttgctat cttgtttaaa
     8101 tcttaacact ttattttcta tgcttccgtg ataattatca cagtttcaaa aataataaaa
     8161 aaaatattaa tggagtttat taagttctgt taatattttt ttattatcta cattattcaa
     8221 tttcaaatct aatcacttaa atacaatcat taagtacaat aaaactacta tctactcaat
     8281 tttttgaata aaaaagaatc ttatttgttt ttttatacaa ttcaataggg taaaataaaa
     8341 attaataaat tagacaataa aaaagaatct ataaatagat tctcttaata atatttttat
     8401 ttaattaaaa ttctaataat actcttactt aatcataatt aattattttt aaaatacctt
     8461 gataattcaa tttctttaac ataaaaacct ttattgtcaa ttttaataag ttcttctttt
     8521 tgcaatacct ttatcattct ggaaacagtc tcccttttac agccaagcat atcagctaaa
     8581 tatgtaactg taatatttac atttagtaaa gtccattctc cacagcaaac tccaaattct
     8641 ctacttaacc tatataattt tgctgcaagt tttttatcta cttttatgga tatagaattt
     8701 ttcaactgtc tatataaacg tcttattctt ctttccatat aagaaataat atcctttgta
     8761 agttgaaaat cattttgcat aagttctaaa aattcttcaa tactataata agctacaatt
     8821 gaattttcaa atgcttcaca gccaacagta gagtttttag aagcgtcaag tgtaatatca
     8881 tttatgactt ccccactatt taatatgaaa attacttttc tctctccatt ttcacttatc
     8941 ttaaaaatag atacctttcc ttctaaaagt atataaacac tgtctaagct atctttttca
     9001 taaaataaaa tttctttttt ttgtaatctt tttagtttaa ctttttcttt aagttgattc
     9061 ttcgttttag tacttaaatt attaaaaatt attactttat caatattata atcttttaat
     9121 ttagacatgt aatatctctc cattcctaaa tttttaagag agctctataa aacattacaa
     9181 atttcttatt ttattcattt ttaaacaatt ttatagcatt tgtagtttta attttccttt
     9241 caatagcttc attagaaagt tcttttacta cctgtgtata tactaaatca ataatgaaaa
     9301 attgtgctaa tttagaagat attgaaccgg tttccagtac tgtttctccc gatacataag
     9361 ataagtgtac atctgaaata tcctttaatt cagaattttt attttcagta attgatataa
     9421 ttttagcatt attggctctt gcaagcttta cagtttttat aatctcttct gtttcaccag
     9481 aatgtgaaat tgcaattatt aaatctcctt cttccattat ggaagacatc attatcattg
     9541 tatgactact gtcaaaactt acacaattaa gacctattct cataaattta tagtttgaat
     9601 cctgtgctat tataccagaa tatccaatac ctatgaagta aactttcttt gcatttatta
     9661 taagccttgc gcagtcatgt acatccttac tattgattat ctctactgtg ttctctaaag
     9721 tggttacatt tgaacttaaa agtttttttg ctgtatccaa agctggctca tcattttgta
     9781 tatttttatt tataatattt tttttattga tagttgatat ttcttgtgct aaagtaactt
     9841 taaaatcctg taatccatta aaattcattt tttttacaaa cctagttata gttgcttcac
     9901 caatattgct ctctttagct atttgtgata ttggtttata aaatacatca tctatatttt
     9961 cttttatata agctattaat aatttatctg attttgtgac tttaaaagtt ggtgattcca
    10021 attgttctaa aatactcatt aaaaaccctt ctattcttta tattttgttt aaaaaattat
    10081 ataatgctcc aagcattcct gcatcatttt gtctctttgc aaattctata ttagtgtttt
    10141 tgtaaactct ctctataaga acctccttta atgctttgtc tatttttggt tttaggaact
    10201 tttcttgtgc cataatccca ccacctaaaa ttaccacttc tggatttatt acataacata
    10261 tatttgcgat tccaactgct aatgatttta tcatattatc gagttctttt aaacaatctt
    10321 catcattatt ctttgctaaa tcaaaaatta tttttccatt taagtcattt tcattcaaat
    10381 tttttatttt tgctactttt ttaattagac tgctagtaga tgccaattct tgaaaagaac
    10441 ttccatttac attcatatat cctacttcac cagcactatt actaaaccca tttatcagtt
    10501 tattatttat tataatacat ccaccaattc cagtacctat tgttaaacat atagaagatg
    10561 ttgcacctct acctgctcca agccacattt caccaagtcc agcacaatta acatcatttt
    10621 caacttcaca ttttatatta aactcttttt ctacttcttt ttttaattgc atgcccttat
    10681 agtttggaat taattcttct aatgcaaata gtattttacc ttcaactggg tcaaccatac
    10741 ctgcagttga tatacatata ccacttattt cattttcctc tatatatttt tttactatat
    10801 cttttatttt ggctaatatt ccttctccac ctttttcctt tgcttcagta tcaagacttg
    10861 atttatcaat tataaatcca tcttctctaa taaaaccata ttttattgaa gtacccccaa
    10921 catctataca aacgtaattt ttcataacat atctccatac aaattatttt tataaattaa
    10981 caactctatt taaaatttta tattacacaa atcttttact tatcttttcc aattcacaga
    11041 tatatttcct tgcttctgat aagtctatag tatcacttaa tacacttttt tctttggtta
    11101 tatgatactt ataaatattt ttacataagt cctctcctaa catattaaga ccttcttgag
    11161 tgtaatgaac atcatcaata gttaatcctc tgtcatgaaa ctgcatagca attttgcttc
    11221 ctacacataa tatatcacat tcactacaag ccgactcctg tgctgctgca attcgtcttg
    11281 ttctaataaa atattcatct tctccaagcc aataaccagt taaacttaca aacattttat
    11341 caatcataca ttcttcttta agcctatacg caattttttt tagactttct ttatagtaca
    11401 atacatcact accatatttt gcatcacttt caccttgaat ccaaataacg tatttatttt
    11461 ctatattata atgtttactt aaggtagtac agcctgtatt aaatttttct atagtgtcat
    11521 ttagaaacca gttattatca tgattccagt tttttattct tgaaccatct tttgcattgt
    11581 gaataaaaca aactttattt ccagtaagct tattccattc atttgaaaat gcaggtgcta
    11641 tagtaccatc accttctgaa gtctctagct cacatctcat atttataatt tcatcagtac
    11701 tcaaatattc aaaacaattt tcatttggaa tgatgctctc ttttggattt cccaatcctc
    11761 tagcattgct ttgtcctatt aataagaata catctatatt ttccataagt taactccttt
    11821 acaaaatttt aagcttttaa ttattttcaa ttttctcata aaaaactaag gctgaaaata
    11881 gtaatctaac taaaatttac gaagtctcac taatcgctga aagttaaatc actattttca
    11941 gccctagtgg ttatataaat tatcattatc taaccaaaac cttaaaaata gcttttttga
    12001 tattttcttt ttctaaaact tttattcctg tcatatgagc atcttccgga taacaaacta
    12061 aaaaatcacc ttttctaagt tccatgtatc cattattttt accatcaaga ggcaagaaat
    12121 cgcccttttc ttcatatatt ttttgttcta aattttctat aaaatttata tctattcgtt
    12181 cacatccatc aagcatcaaa tgaacatcta tatacttctt atgagcttcc cagaatctat
    12241 cttctttttc acaagtttta tactctacaa tattcacaaa aatatcatca ccatctattg
    12301 tataagtacc tttttcataa tctattaatg aattatcttt agcatattca aaacatctaa
    12361 gtaaatcttc atgcaatgtt gaatatgtct tactatgatt tatatttcca aatatcataa
    12421 gcactatctc cctatataat tataaagtct atttttatac ttt
//
LOCUS       DAKBNN010000115        12442 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000115 DAKBNN010000000
VERSION     DAKBNN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.116"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(91..378)
                     /locus_tag="OUB02_002548"
     CDS             complement(91..378)
                     /locus_tag="OUB02_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886294.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(398..673)
                     /locus_tag="OUB02_002549"
     CDS             complement(398..673)
                     /locus_tag="OUB02_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886295.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(1310..1489)
                     /locus_tag="OUB02_002550"
     CDS             complement(1310..1489)
                     /locus_tag="OUB02_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886296.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(1774..3240)
                     /locus_tag="OUB02_002551"
     CDS             complement(1774..3240)
                     /locus_tag="OUB02_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8886297.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            4228..5406
                     /locus_tag="OUB02_002552"
     CDS             4228..5406
                     /locus_tag="OUB02_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8886298.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYRLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYINNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            5756..6637
                     /locus_tag="OUB02_002553"
     CDS             5756..6637
                     /locus_tag="OUB02_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8886299.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            6828..6995
                     /locus_tag="OUB02_002554"
                     /pseudo
     CDS             6828..6995
                     /locus_tag="OUB02_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(7255..9822)
                     /locus_tag="OUB02_002555"
     CDS             complement(7255..9822)
                     /locus_tag="OUB02_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HCT8886300.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(9825..10430)
                     /locus_tag="OUB02_002556"
     CDS             complement(9825..10430)
                     /locus_tag="OUB02_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8886301.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(10804..12264)
                     /locus_tag="OUB02_002557"
     CDS             complement(10804..12264)
                     /locus_tag="OUB02_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HCT8886302.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
BASE COUNT         4236 a         2131 c         1569 g         4506 t
ORIGIN      
        1 agttcaacta tactggttta aataggtttc ctttagttta aataattcac ctatacctac
       61 ttgaatagtt cacttttatt tgcttaaatt ctataccaat actggcttaa acattatact
      121 accatgattg tattgtctat cttcaactac aataaaaaca ggttctaaat ctttagtaga
      181 tgttattttt aattctaaaa aaacattatc agttggtaat atttttacat cataattatt
      241 taaattatta ttcgtgatac tattaaatac atcaccttga cgttcaaaaa aaacattatc
      301 aactattaca aaataaacat cttcttttct actattttca acctctatag tattttgttt
      361 agttacattt agtctcatct tccacctcct attaatatta cttaattata ttaatacttg
      421 taattgttgc attatgttca tcacaaggaa gaggtttaaa aacaactatc tctgctaaat
      481 aattctgtgg aggatttgta actgaagtag cattgcaaat aaatttaaaa tttgttcctg
      541 ctacaacttg agtagaaaca ataagtggtt cataaccaac cccttctaac attcctacag
      601 cttcattaaa tacttttaaa tcttcttctt cgacagctct gaattttgac catccaccaa
      661 caaaaagact catataaaaa cctcctttat atatatattt atttatatat atagtagtac
      721 caattttctc aatattcaat attttctgtg taactggtca atgaaaatgc ataactggat
      781 aaaaatactt ataaaataac ttaactttac atataaatag cttactttac atgtaataag
      841 attattctac aatatgaaat gcatcttgtt ttgaatatat tctaaataca ataatggata
      901 ttttcaatta ttataattag aaaatatcca ttacttaact tgttttattt aaaatcacat
      961 agagacttta ttattttttt tattgcatga atagctattt caggtatcat aagttgatat
     1021 accccctcct tgaaaccaca aaaaacataa caaactttac gaatcttgtc ttaaaagacc
     1081 acaggtacat tccttatttg tggattggag tagccataac aagcaaattt tacaattttt
     1141 atgatgacaa aaatcaataa aatcgcttag tatataacac gaaacactac ttgttactat
     1201 taagaatagt tagataggaa gtatgcatta taccaataaa ggaatcaata aagaaagtta
     1261 aaacatccct acaaatcaaa aatctgttac atttttctat atttaaacgt tatttactta
     1321 caattggaga aataacaggt tttccttctc ggtcaagtaa aggcgtaaat ccggctgcat
     1381 ggccataata gtggaataca tagttcacac ctgtttcttt atccacccaa atctccgttg
     1441 catttatttt tccttgtgaa tatacttttt caaatctatc ttttcccatt ttataatcct
     1501 ccatattttt gattggtcta tgccttcttc acttaagtag aaaaaataca taacttattt
     1561 ttttaattct aatatgagca cgtaaaaatg tcaaacagct tatttaattt cataacttca
     1621 tgttactgtt cgtaaccttg tctgtttact tacactgcat tacagcacca ttttatcctt
     1681 tacattattg tattgcacac agtaaaaatg cttactttat gggatttttc aactaaaaaa
     1741 aggattttat ctccacaaaa catttactat ttgttacaat atacctaatt ttaatctcat
     1801 atcaacattt aatgaagata taaccttttt caaatattgt aaagactcct taaaaatatt
     1861 agaaaaagat tctgcaaatc tctcaacatc tccttttttt aaatacctaa gtatctcaaa
     1921 acatacatca aaacgtgttt tcaaaatttc attatattct tcatgaaaaa atctctctaa
     1981 atagattcca aatatcatat attgttttaa atgcaaaaat gtttcctgta atacataaat
     2041 ttgtgtataa tctactatct tacaaagtaa aacaaaatct ggataaacct tacactcttc
     2101 acaattattt tgtaagtacc ttaaatgagt ttctatttta tctacagcta tcaaatccat
     2161 tttatttcct agtaaaagta ctacatcctc aagagtaatc gtaacaaact ccatagcatc
     2221 caaaagcaaa ctcaaccttt tagctccttc ttctgtattt atataatcat catcatattc
     2281 agaaaaaagt gttacatatg ttcccttgcc attaattgtt ttagcaattc ccaattcatt
     2341 tagctcatta taggcacttc taactgttgg aagagataca ttatatctat taattatcga
     2401 agtaattgat ggtagacaat ctcctatttt aatattgcct tcaaaaattt gacaaattac
     2461 atctgaaaca attaagtcat ataaataaca tttttccaac gttacagaat taaaactact
     2521 tgtatctata ctttgtggag cacgattttc taccatttta agtatggatt ctctacaagc
     2581 tttatacata tatgttaata gttttttaaa ttcaattaga ttcccgtcca atccatataa
     2641 gtaagttttt cttacctgct ctatgtattt attacgaaaa aagtctatac aacaattatc
     2701 caagttatcg aaattatagg ccaattgtga aataatgaca tcatgctgaa taatctcaca
     2761 aaaatggtga atcaggaaat tatttgtttt acttattagt ttatttatat attttagcct
     2821 ttgttcttta aagactaaat aattagaact atttttttcc atacaatcga taatctcgtt
     2881 taattcttca aagtctttag atgtaaatct tttaatattg tacatagcaa tagaaggaaa
     2941 tattaaacta aaaatcttat atatttctga tatttgtaaa atcttctcat ataataagtc
     3001 agattctatt gattgtttac tcgtactctg gattaaaact ggtctagaac cagcttttgt
     3061 ctctataaag cccttttctg agagcatttt tattacagtt tttatagtat tttttgatac
     3121 catatattct tctgacatgc tttttataga atcaagccta tctccaatag taattttacc
     3181 agtataaatt tgttgaatta tatgctcaaa aataattaaa tattgtggtt ttctcttcat
     3241 atttttcctc ttcgataaat tagatttatt gttttttgta aggtatattt acttttattt
     3301 aatagggcaa aatagttgct tattttttcc tcataatcta taattaatat aaaccattta
     3361 tatatatggt ctaagtttta taatactgta atgcagtaaa atagcatatt gtagctatac
     3421 tacagcaaat taacatacca ttgtagtata ttaattggaa taattttaag tacttcaaaa
     3481 ttccagcaaa acatatatgt gtttttgata ttatgtatat tcgttaatag aatagctata
     3541 cttgttttac ataataaatt atatgatact tgctatatgt aattactata tttaatacac
     3601 ataactatta tacttactat atgtaattac tatatttgat acacataatt attatactta
     3661 ctatatataa ttactatact tggacacata actattatac ttgctatatg taattactat
     3721 actttataca catagttatc atacatgcta tatataatta ctacacttgg acacataact
     3781 actatattta ctatatgtaa ttatactata cttggacata tagttatcat acatactata
     3841 tgtaattact atacttggac aaataattat tatatttgct atatgtaatt atactatact
     3901 tggacatata gttatcatac atactatata taattactat acttggacac atagttatca
     3961 tacatgctat atgtaattac tatacttata cacataacta ttatactttc catacataat
     4021 tactatattt ggacacatag ttatcataca tactgtatat taagatataa tactttttaa
     4081 attttatata acacagacac atgcaataaa aaagaaggta tccataataa gctctttgca
     4141 tgtacctaaa taaaaactgt aaaatagaat atataaaatc taatctattt taagtaaata
     4201 caatttcact ttaaaagagg atgtactatg aaataccata tatttttaat cataactact
     4261 atattaataa tcgaactatg taacatttac atatgcaaaa tagcatcttt tggaaacaaa
     4321 aatataacta tacataagaa ttacaaatat atttttttaa taaaaccatt aaccttcttg
     4381 ataataatat tcacaaaatt accattacca ctatattata tattgtttta catagtaagt
     4441 cataaattgc tcttaagcag aaaaagttct actaacaacc attttattcc caatatggca
     4501 gtaataagtt tttgtatttt acaccttatt actgtaagta caatatcact tgttaaaaat
     4561 acaagtccag atataatatt tgcaaatatg acctataggt taattagtat tttaatagct
     4621 tctattttag attttatctt atggtactct atgaataaaa taaaagagca tatttatata
     4681 ataactcaaa atcaaataga gttcagtcta tttttaaaat ttatctggtt cactttagga
     4741 tatatattat ttgatagtat acaaattaca tttgctccac caagttctct aacttcctac
     4801 tttatattgg gaagtaacat attattaatc ttacaactaa ttatattcat caatcatata
     4861 tattccattg aaaaaaatat ttatttagaa aaagaacatc atagactaaa aatgataaaa
     4921 caagaacaat tactaaaagt aaatttatta aagaaaaaag ctaacattga tgaattaaca
     4981 tcagcatata atcgaaaatt tgcttttgaa aaaatacagc attacattaa taacaattta
     5041 ttattttcat tgatatatat cgatttggat aaattaaaga cgataaatga tacatatgga
     5101 catttagctg gggataacta tattattaac ttctcaagta tagttattga aaacttagga
     5161 atagatgaca tatttgctag aataggagga gatgagttta tagttattat gcctaaatct
     5221 aatgaaaaca ccacaaaaga atttatagag aaaataagta aaattatgga taatacgata
     5281 tcttttagct atggtattgt agaagtttct agcgaaaaca aaatgactgt agaagaaatt
     5341 attaatattg ctgatattag aatgtacact aataaacaaa ataatcataa aaggaacaat
     5401 aactaaaatg aatatttaca tgagcttact tgtagtctca ctatgttttt tatcttatta
     5461 tctctatcat tataattttt ttaaatatct ggacataatg attaataact ctaaaaataa
     5521 atacacagta tatattatat attctatatt taattattta atttttatta tttgtactta
     5581 tttaaagttt catctaatta taaattggac aatttttatg ttgattttat tcatacaaat
     5641 taaagtaact tataaaatct ccacaataaa aaacattttt tattcactaa actgctccat
     5701 cattggttta gcaataaata tattttttag gtctttaatg tcaatagtgt taaatatgcc
     5761 agcatattta tttgatagta attctattgc aaatatttac agagtatttc ctatatcatt
     5821 tagctttata gttacaggac tatatttcag ttataaaaat aataaaaata ataagattaa
     5881 ctttgaggca gttttagagg acaaaaaaaa cttaattttc ttattgagcc ttatgtttgg
     5941 aatgtacttg tatctatgtt tgagtttgtc aatatactat actgttggaa atgtattgat
     6001 tttaaaatta tatggaatca agtctgccat aatagttttg gtaggtaact atattgcaga
     6061 aagatatagc tttagaatta ctcaaataaa aggttatgat attaaaaatc aaatagaata
     6121 tgtagaaagt ataaagacag agtatgaaat taataaattg aatgatattg tatatacaga
     6181 tttactgaca ggcttctaca atagaccttt tgtaaatcaa aaactatcag agtggacatc
     6241 aaatcactac aaatttacct tatgttttac tgatttaaat agcttaaaac ctgtaaatga
     6301 taaatttgga caccaatttg gtgataaata catatccact actgctcaaa taataaaaaa
     6361 acttataaat gtagaaaatt ccttactatt tagatatggt ggagatgaat ttttattcct
     6421 tttacagaat acaagttgtg tagaagcaga aaaaataatg attaatataa attatgaatt
     6481 atcaaaatta agtaatactg acgagtatcc ttatgctctt tccataagct atggattagt
     6541 tgagtgggat ggaatatccg atattgaaac tttaatatca aaggcagatt ctttgatgta
     6601 tgaaaataag cttaaaaata aatctacttt aaactagcta gttcagatat tctacaaaat
     6661 atatgttata agagagtact aagaatgtca aaatatatag attttgcaaa tattgaatac
     6721 agtttactaa tgaatgcact ggatgtaagt gttagtaaac acctattaga tgaacacttt
     6781 actgttgttt gggctaatga ccattattat gaaatgttcg gctataccta gctaaaaagt
     6841 taaaaatgac cactgtagcc gagggaatag agaccagttt tcaaaaagat ttcttacaag
     6901 aaatagactg tgatatgtta caaggatacg tgttctctag acctttacca ataaaagact
     6961 ttgaaaaact tatgtttcaa aactcctcca attaacaata taaaattgct tattataagt
     7021 gatattattg tatcaacaaa taaaggaatt gtctcaaaat tgattttttc taatcaaatt
     7081 tttattttga gacattctca tttttttgca taagaaaata cccaccaagc tacggttttt
     7141 cttaccgctg ttcggtgggt attagtagta ttatgaaatc tgaaaacttt aagtattact
     7201 acttccgcta gcgcaatcac aaaggtcgaa agcttcaaag cgaaaatagg ctttttactc
     7261 atcaaacgtc accgtacctg ccaacccatc caccgttatc atttgtccgc tcttaatagt
     7321 tcgtgtggca atgtgtgtag ccataacggc tgggatgcca tattcacgtg cgacaatgct
     7381 tgaatggcta agaggaccac caatgtccgt tacaatcgca cttgcagatg aaaataatgg
     7441 tgtccatgct ggagttgtgg taactgcaac caaaacactt ccaggttgaa agctttcaaa
     7501 atctgctgga ctattcagta cacaagctgg agccgtcaca acaccagtac tggtaccaat
     7561 gcccgtcaac acaattttcc catctttctg cttctgtggt gcttgtgata tagttttttt
     7621 gtttttttct ggcaacttgg atggtgacac ataacccatg tattttttaa gttcagcttt
     7681 tcgaacaggt attatcccgt tcatatcaga tagagacttg tttttatcca actgctctat
     7741 aagtacctcc aattccgatt ttgtgagcca ataaatatca tctaaatgtg aaatagctcc
     7801 tcctcgtatg aatcgttctg agatttcctg aaacatacaa cgaatcagag gatgtcccat
     7861 tcccatcaaa taaatagcat tttcacgcat aggagaggtt tcctgtgccc aatggagcaa
     7921 cttaaaaaat agttttttac gcaatccacc tatgtgttgt aaaatctctt cttctgcatg
     7981 ttttctacgc ttttcaaacg tggtctggcg taaaaaagga ctttcccctt ttccttccac
     8041 aaatgttttt acggattcaa aagttggtgt aagtgtttcc tgcggtgtgg aatgcgcaaa
     8101 atcaaactca taagcggtac gaccaaactc tttaaagtac ttattgattc gatttttcca
     8161 ttctatccat acttcctgtg aaactccaac aggcacaact gaagacatga aatcttctgc
     8221 tatctttatt gcaggactgt tttgcagata aaggttgatg ctgttatttt gctttaccca
     8281 ttctgaaata ccccacaact ccttttccga ttgcagaaca atcgtatcaa acccaagcag
     8341 gaaaacggaa gtatccgtta taccagcacg tcgaactgct ccttgaaata attttgtaaa
     8401 taatgtttca ctgatagaag cagcaggcaa tgtaagctga atccttgtga aataaataca
     8461 tgcggcataa aatattgtct gaataccctc cataatttga tgggcgttca tagtatgcag
     8521 gggcttttct tcccattgtt taatagcatc ctcatatcct tttcgtgccg tttcccagcg
     8581 tgcaacgctg ttcataagtg tcctacgtaa agagcgtggg gaaagggatt tgatagcaat
     8641 caaaagtgga tttgatttag cagaaagata aacatatcca ttaataaccg tatatgcccc
     8701 gttttttggt aataattttt tcgcactttt accaaacata tctatccata aaagctctga
     8761 tgcacggttt acgatttcaa gaccaagcgt ggcgaacaaa ggtgtaacag gatttggcaa
     8821 atgttctgcc aaacttccct ttgtgtatac aacatcctgt tctggcaata cccattctgg
     8881 tggcagaaca gtaattggac gagcctgaac aatgtacaat ttatcttttt ccagtgccca
     8941 ctctacatcc ataggcattt gataatactt ctcaattttc tttccaagct gtatcagttg
     9001 cattacctga ttacgagtaa gagcatattt ttttctcaac ctttcaggaa ccatggtttc
     9061 ttctgttcct tctgaagtac gaaccgtcat aatctcttta tttgccactt catacgatat
     9121 aattcgttcg gaatccttat ccactacgat tgtatcgggt gtaactaagc tagaaaccac
     9181 cgcttcacca agcccccatg cagcgttaat aatcatttcg ctacgtctac cattgattgg
     9241 gtttaacgta aacataatac cagatgaatc ggaaaatgca agcttttgta ctacaacagc
     9301 aagtgcgaca atctcgtgct ttatattatt cttcacacga taagcaatag cacgagctgt
     9361 ccataaggaa gcccagcatc tctttacagc gtcaataact ttatcttcac cttgtatgtt
     9421 aagataagtt tcctgctgac ctgcaaaaga agcgtcaggt aaatcttcag cagtcgcaga
     9481 cgaacggaca gccaccgata tgttccctaa tccggcataa gctgttttaa ttgcgtccga
     9541 cacttcctgt ggcatttctc cattatggaa aagctcccct attttcttag atacatcttc
     9601 aagttggctg gtattattgg aatcaatacc atctagcagt ttattaattc gagactgaat
     9661 atggttcgtc tcaacaaaaa tctggtatga agcagtcgta acatgaaaac cgtctggcac
     9721 agggatgcct gctgtcaata gttttgataa agacatccct tttccaccaa caatttcaat
     9781 ggttgcttgt ttatgttcca gtggcaagac ataataaatc ataatcattc acctccatca
     9841 ttcataattc cgtgcagaaa tatattcatt gtttcatcca acattttttc cgaactatcc
     9901 aatggtcttg acgtgtaact gattgataac atggagttaa ttaacaaacg tgtcacaaga
     9961 gcggtatccg ttttgcgaaa gatgcctgtg gtcacaccat tatcaattac cgattttata
    10021 atatcttgat atgcataacg agcatcttta agtctctttc gatactcttc ttctaaataa
    10081 tcagattctg tatttaacca catcagtaca gttctatact gctctggaaa tctaagatga
    10141 ttaagcatga tttttctaag gcactgttgt ggtgaaggtt caccatcaat aatcctatga
    10201 acttgttgga tagttttttc tatttctttc tcaactgcat atacaacaat ctcatctttc
    10261 gttttgaaaa aatcgtatag acttgacttt cccatgttgg ctaaaactgc aatttctctc
    10321 attgatgttt tttggaatcc attttcagcc atgagactca aagcaacagc aactattttt
    10381 tctcggcgtt ctgcttgttg ttcgggtgta agcacaattc cttttggcat agggcgatac
    10441 ctccttttag cgaccactgg tcgtttcttt tttatttatc atacagcgac caacggtcac
    10501 ttgtcaaggt taattttttt cattgtatat ggtactcgag ataaaaatgt tcattttaga
    10561 aattgctcta tttaagtaga aataggcttc atcattagaa aacaagacta taaatttgag
    10621 atatttatca tgttcaaatt tcatcaatat caaagtttaa aatattaata aaaaaagaat
    10681 tactaagaaa taacatataa agattcaaaa aaggagttga acttaaaatt taattacaaa
    10741 aaatcaaatt taagacaact cctttattct ttatctctac tattctttat ctctatttat
    10801 ttattacaac tccctaacat ttaccactac tgccttaggt ctagtcatag cctcaattga
    10861 atacttaaca ccttgagttc ccataccaga tgcctttaca cctaaaaatg ggaagtggtc
    10921 tggacctctt tcagtcttat tatttatttg aacagtacca acttcaagtt tatcagctat
    10981 ataaaatgcc ttatcaatat cacttgtaaa tactgatgat tgaagcccat actctgattg
    11041 attagcaatt tcaattgctt gatttatatc tttaactctt ataattggaa gtactggacc
    11101 aaatggttct tcccaagcaa ttttcatatc aacatttaca ttatctaaca atgttggata
    11161 gaataaatta ttttttctaa cattgcctgt tattaacttt gctcctttat gtaatgcatc
    11221 atcaaccaac ccttgaacaa aatcagttgc tttattatct ataagaggag tgataactac
    11281 ttcatccatt ggatttccaa ttgtcaactt ctctaccaat ggctttattt ttccgactaa
    11341 tttatccgcc acactctctt gtactaaaat tctctttact gccgtacatc tttgtcctga
    11401 atatgaatac gctccagata ctatattctt agctgcaaaa tctaagtctg catcttctaa
    11461 tactatagcc gcatcttttc caccaagctc caataataat ggtaccattc cagatatttt
    11521 agatatatgc tgaccaactt cagtactacc agtaaagtta ataaaattaa ttcctttatg
    11581 cgttacaaca taatctccta tctcactacc cctacctgta atagtattaa gtactccatc
    11641 tggtatccca gcttttctca taatttctac cacatgaagt gcgctaatag ccccttgtgt
    11701 agcaggtttt aatacgactg catttccacc aataagtgct ggagcaatct ttgacattga
    11761 taaatttaca ggataattaa aaggagatat agctaaaaca gtacctaatg gaacccttga
    11821 aacataagac atcttatttc ttgttccacc agggaaatta tctccactaa tggcttcacc
    11881 ctccatattt tttcccacat cagctgtgta tcttaaaaaa tctgctgttc tctcaacttc
    11941 tgcccttgca gattttatgt ctttggctat ttcaagtacc agtatattag ctatctcatc
    12001 tatattttct aacaataaat cagcagcctt atgaaatata ttggctctct tgtatacagg
    12061 catttcagcc catactttta tactctcttt tgtgtttttt ataacctcat ctacctcatg
    12121 ttttgtcata gattgtactt tcccaacaag agaattatct attggtgagt aaatttctat
    12181 tggctttctc gaattagatt ctacccattt tccatttatt aaatttttat atacattttc
    12241 ttttgtttta agttcattaa acatattttt attcctctct ctgatataac tttgtgtatt
    12301 taaattatag ccatttattc gttatgtaaa catttctttt ttccataaac gtttacctat
    12361 aaaaaaaatt ttaagttaac gtttttctac attcttctat tttaataaca tactcgctcc
    12421 aaatctatat aggtcacact tt
//
LOCUS       DAKBNN010000116        12267 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000116 DAKBNN010000000
VERSION     DAKBNN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.117"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            352..3450
                     /locus_tag="OUB02_002558"
     CDS             352..3450
                     /locus_tag="OUB02_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HCT8886303.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3573..4859
                     /locus_tag="OUB02_002559"
     CDS             3573..4859
                     /locus_tag="OUB02_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT8886304.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5237..6343)
                     /locus_tag="OUB02_002560"
     CDS             complement(5237..6343)
                     /locus_tag="OUB02_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCT8886305.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6378..7919)
                     /locus_tag="OUB02_002561"
     CDS             complement(6378..7919)
                     /locus_tag="OUB02_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCT8886306.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8281..9027)
                     /locus_tag="OUB02_002562"
     CDS             complement(8281..9027)
                     /locus_tag="OUB02_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8886307.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     ECSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9239..10549)
                     /locus_tag="OUB02_002563"
     CDS             complement(9239..10549)
                     /locus_tag="OUB02_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886308.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10562..11032)
                     /locus_tag="OUB02_002564"
     CDS             complement(10562..11032)
                     /locus_tag="OUB02_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886309.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(11036..11887)
                     /locus_tag="OUB02_002565"
     CDS             complement(11036..11887)
                     /locus_tag="OUB02_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HCT8886310.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFSMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
BASE COUNT         4538 a         1677 c         1845 g         4207 t
ORIGIN      
        1 taatttatta ttgaagaaat tttaatatat aatagtttta tatttttaaa tgaatgaagt
       61 attatcctca tatatgattg gaatacttaa ttgaggtgga gatttgtcat ttatatgaga
      121 attaattgta aatacataat aagatagtta attataaata tattaaatta agtattacaa
      181 aactctattg tacgattgac acaataataa ttctatttac taaaaaatag aacctcatta
      241 aggtactatt ttaaaacata aagaagtata atatcattgc caaatatata gcaaaatttt
      301 aaaagcttta aaaattcaat ctggaaaatg aataaaagga gatatatata aatggatgta
      361 ataaatttaa tagatgagtt ggatgaaatt gtatatgttt cttctttaga tacatatgaa
      421 cttttatata ttaatgaaat tgggaaaaag gcaatgggac ttaatgatat atcccattta
      481 aaatgttata aagcattaca ggggaaagat tctccctgcg aattttgtac aaactctatt
      541 ttaaaagaag atgaatatta catatgggaa aatacgaata ctcgaacaaa tagacatttt
      601 atattaaagg acaagttaat taaatgggag ggcaaacatg cacgattaga aatagctctt
      661 gatattacag aaaaggaaga tataagtaag tcaatagcag aaaaattaga gataaaggag
      721 ttattagttg gatgtattaa acatcttata acagaatcta atttttctga tgctgtaaat
      781 cttgtattaa ccaatattgg taaatttcat aaagctgata gagcatatgt ttttgaagta
      841 tcagaagata gaacaaaagt aactaataca tatgaatggt gcaatgaagg tgttgatgaa
      901 caaaaacagg cactaacaaa tcttgatatt aatgaattaa agaattggtt tttattattc
      961 gaacaaaaag gttttgtaat aattgatgat atagaaaata taaaagaaga acagccaata
     1021 gaatatgaaa ttttaaaacc tcaaaaaatt gaaagtttaa tgacatcttc actaaaacaa
     1081 gatgataaag tagtagggtt tattggagtt gataatccac ataaagctgt tggagatacc
     1141 tctcttttga catccctatc ttatttttta ttaactgaga tgaaaaaaag aaaagtagaa
     1201 aataaattaa attatatgag ctactatgac ttcttgactg gtgcatataa tagaaataaa
     1261 tatattaagt atatagataa ttttcaaaaa ggaaaagtta aatccatagg agttgtattt
     1321 gtagatataa atgggttaaa agatattaat gataactttg gacatgctaa tggcgattct
     1381 gctattgtgt cagcatgtgt aataataaaa aaatactttc attcttataa tgtttaccgt
     1441 attggaggag atgagtttgt tgtattatgt gaagatatat caaaacaact atttgaagaa
     1501 ttagtgagtt ggttaagtga agaatttaag gatgagatga tatatagtgt agcagtgggc
     1561 agtatttgga gtgacgataa tattgatata cataagctaa ttaaagttgc tgatgagaga
     1621 atgtaccaag ataagaagag gtattataat aagaacaaac ttagaaataa tgaaatgaaa
     1681 gtaattagtg acttagctga ttcgacgaat aatattttat ttaaacataa agagttagtt
     1741 gataaggttt attttgatga tttaatgata aaagggtatt ctaaagctta tgaaaaatta
     1801 gcttataata catatgatat aattttatct atttgcattg atacgaattt aattactcca
     1861 atttatcaat cagagagcaa tatttttagt tttcctataa aggaaaatct tgaaaaaact
     1921 ttttcataca taaaaaaata tttaattcat cctgatgata taatattatt taatgataac
     1981 caccttctaa aatctcttga tagtcttaat aatagcaagc aagacgattt agaactaaat
     2041 tttgagtata gacgtttagg aatagataaa aagtactatt gggcattaat ttatatgtta
     2101 aagattagta gtaaaaataa gttaggtgag aaacaaacac atattttagt tgctataaaa
     2161 aatatagatt atcttatgca actacataaa aaccaaaaag atacattaac aggactatac
     2221 aattatgaaa gtttttgtga acaaataagt ttaatgctta aaagatatcc tgacaagcgt
     2281 tatgcaatta taatgatgga tatagataag ttcaaggtaa ttaatgacat ttatggagtt
     2341 agaggaggag ataaagcttt aatatatgtt gcagagttga ttcaaaaaaa cattggttct
     2401 aatggtatat gttgtcgtat gtatgcagat gtattttgca ttttttatga gagttactct
     2461 gatagagata ttcataaggt aatttataag attaaaaata gcttaaatag aaaaaaattt
     2521 gaatatatgt tagtaccatg ttttggggtt tatagaattt tggataatca tattccaatt
     2581 acaaatgcgt gtgaaatggc aggatatgct cacaaaaaag taaagaaaca aagtatcaat
     2641 aactatatat tttatgatga tactataaga aatgatgcac ttgaagaaaa acagatggaa
     2701 agggaaatgg aatacgcact tgaaaatggg caattccatg tatatttaca acctaagtat
     2761 aatcttaatg caaataaaat tgttggtgca gaagcattgg ttagatgggt tcatccagaa
     2821 aaaggtataa tacctccaat taaatttatt tcactatttg agaagaatgg gtttattatc
     2881 agattagata tgtatgtatg ggaacaagta tgttctttga ttagaaaatg gatagattta
     2941 ggtgaagagc cagttcctat ctctgtaaat gtatcaagat tacatttgca taatccacat
     3001 tttaaagaaa ttgttttaag tttaataaat aaatatagta taccaaaatc ttttttagaa
     3061 ttagagctta cagaaagttt atttattaaa aatataaaat tatttactaa gataatagcc
     3121 gaccttagaa tggatggtat tgttttaaat atggatgact ttggttcagc atattcttca
     3181 ttaaatatgt taaaaaatat taatattgat acattaaaga tagattgtgg tttctttaat
     3241 gaggaaggaa tcacagacag agaaaaaata gttttaaagc acactatagc catggcaaaa
     3301 gatttagata tggatgtagt ggcagaaggt gtagagacaa aagaacaagc tgaattttta
     3361 gtgagtttag attgtgtggt aattcaaggt tattattatt gtaagcctat tccaagtgct
     3421 gattttgaga agttacttta tcaaaaataa gctaattatt ccattctaaa tgcagatagg
     3481 atggagtagt tcaaaataat taaatagaag tagaaaatag tgaggatata atttttaaaa
     3541 agtataatat ttatggatta ggagcgaagt ttatgatagt gggtattata ggtccttcag
     3601 actctgggtt taagataaag gatgatttaa aacagataga cagtgattta aagactaaaa
     3661 tatatgtaag agaaaaagta gtagatacaa tagaagttat ttctaaatgt gaagacgaat
     3721 gtgatgctat tatatttaca ggatgtgcag tttatgaatt tataaaatct aagtatgaga
     3781 taagtaagcc acattcattt gtaccgagaa gtgggacgag tataatgaag gcattttggg
     3841 caataaaaag tgcaaatata aaattagata aattcagtat agatgtagtt gatagatatg
     3901 ttgtggaaga tgcacttaaa gaatttgata ttaaagcaac gagtgtattt tgtaatccat
     3961 tttctttgga agtaggagag tctgaacttg tagaatggca tataaaatta tttgaagaaa
     4021 ataagacgga tataatgtta actggttttg gtgcagtata taatgaatta aaagaaagag
     4081 gctatcctgt atttcgttta caagctacta tacaattaat caaagaaagt tatgacaagg
     4141 taaagagtga atatgctcta agtaaggcaa gattttctca aattgctgtt gaaatattga
     4201 gtttaataga ttataaagag aagatagata actattattc tgacatgata aaaaaatcag
     4261 atatggataa gcttgtagta aactatgtaa gaagcattca aggttcctta tttttacttg
     4321 gaagaaatga ttatgttgta tttgttcata aaggggtagc tgataatgag tataattatg
     4381 ataagctatt caatctaaag agagagataa aagacatggg cttttctctt agtataggaa
     4441 ttggtactgg agtaactgcg tatcaggctg aaaataatgc acataaagca ttaagacact
     4501 ccatagattc aaaagaaatt ggtatatatt tggtagatga agatgaaaac ataagaggac
     4561 ctttagcttc cgaaaatgaa ttaaattatt ctttgatgct atctgatgaa aaggtaatcg
     4621 aaatatctaa aaagactgga atgagttgtg agtcagttgc aaaaattatt gctataaatg
     4681 agaatagaaa aagtaagatt tatgattcaa aagagttagc agagtatcta aatgtaagtg
     4741 aaagaagtgc aagacgtata ttaaataaaa tagtaaatgc aggtttaggt cgaatatgtg
     4801 caaaagagac tagtattggt ggaggaagac caaaaaatat tactgaaata ctgttttaag
     4861 atattttata aaactgtata aatcattata ttgtataaaa ttctacaaca aattactata
     4921 taaagtatta aagcttatta tgaaaaaaat tagaacaaat aattatgtaa aatactagaa
     4981 taaattatta atataaaata ctagagccta ttattaaaaa tgctagaata aattattatg
     5041 caaaatattg aagcttatta tgaaaaatgt tagaacaaat tactatataa aatatcaaag
     5101 cttattatga aaaatgttaa aacaaattat tatataaaat actaaaacct atgtaaaaca
     5161 actctaatat ataacttata aagttgaaga tacataggtt tttattttta taatttaaag
     5221 agttctatta tgatatttat aatttaatca tatgataaac tattttggca gtatcatata
     5281 attcgtctat atttacatat tcattaactg tgtgtacatc atacatacct agacttaata
     5341 tagcacaatt atatccaaga ttagctaata tatttgcgtc acttccacca ccaattacca
     5401 ttggatttgg aacaatacca acttgtctta ttgcttcttc acttaacttg aaaacgtgac
     5461 tatctctact aagttcaaaa ctagggtaag acatattatg ttcaaaagta taagtagtat
     5521 taaatttaga tgcagcatcc ttacaacatt tttccatatg attaagctca tactctaaag
     5581 tttcaggtat atgagaccta atttcagcag tcaatgtaac cttatcagtt actatgtttg
     5641 tagctccacc accttctatt ttacctatat ttgaagtagt caatgaatct attctgccta
     5701 tgtgcatgtt gcttattgca tgagaggcta ctaatatagc atttaaccct ttttcaggtt
     5761 ctataccagc atgtgctttc tttccatgaa aagagatttt tatagattgt tgagcaggag
     5821 ctttgtatgc tatcgaacca atagctccac cagaatctaa aatcaccata tctttacaag
     5881 gcaagttgtc aggattaaag tttttgacac catgcatacc agcttcctca cagattgtaa
     5941 aacaaagata aatatctcta tgtggtattt tttctgtaat aacatgttct aaagcttcaa
     6001 gtatagcagc tattccagct ttatcatctg caccaagtgt agtagttcca tcacttctaa
     6061 ctacattacc atctagtatt ggttttacac ctaaacaagg ttgaacttgg tccatgtgtg
     6121 cacataaaca aagtggtcta cttccttctt caccttttat ataggcaatg acattacctg
     6181 tattgccacc atatttttca cctgcatcat ctataatggc gtctatattt ctttctttta
     6241 agtagtttac caaccactta gacatttcta attcttgatt agatgggcta tcaattctaa
     6301 ccatatccat aaaattattt ataagtctat ttttatctaa cataaattat ttctcctttt
     6361 agaaagattt aaataaatta catgctatat gaaggaagta ttccaggtcc tagtggaata
     6421 ttaaatacca taaacaatat tagtagtata gtccacacta ttaagaatga tatagagtat
     6481 ggtatcatat tagcaattac agtacccata ccagcatctt tatcgtattt tcttgtgaat
     6541 gctagtataa tagggaagta cggaaataat gggctaagtg gatttgttat acaatcacct
     6601 atacgataag ccatctgagt aagtgctgga ttgtatccaa ccatcataaa cattggtaca
     6661 aatataggtg caagaatagc ccactttgca gatgcactac ctataaataa atttataaaa
     6721 catgataata taataaatcc tatcaacaat aaaggtccat ttattcctgc tgattttaaa
     6781 aattctccgc cctttacagc tagtatcaga cctaaattac tatttgtgaa taattgcaaa
     6841 aattgtgaag cagcaaatgc tagtattata tatccaccca tatcactcat tgcactagcc
     6901 attatagaga ctacatcctt atccttcttt attttaccta tagtttttcc gtatacaaca
     6961 ccaggtatga aaaatgctag tgctattata ggtaccatac ctttcataag aggagcattg
     7021 tttgatagta aatctcctgt ttctgggtca gctaagaatg gttttttacc gattacacta
     7081 agagctacta taatagccac atatattaat atacttatac cagctttttt aagccctttc
     7141 ttttctacat cactaagact tccatcaaca tctagttctg catttccttc atatttacca
     7201 aatctaggag ctacaatttt ttctgttacc catgttgcca aaagtattag aacaaaagtt
     7261 gatgctatca taaaaaataa gttcattgtt gcattggctt gaaatccaga atcaatcatt
     7321 tgagctgctg gaactgtaaa acttgcagca agtatatcat tgacacttat cataatgttt
     7381 gcggcaaatc ctagacagac tgctgcatat ccagaaaata aacctataag tggatgtcta
     7441 ccaacaccaa gatatacaag tgcagctaat ggtggaaata atattgtacc tgcatctgaa
     7501 gctatattgg caagcatacc tatagtcatt acaacaagag ttactcttgt ttcaggaatc
     7561 ttgcttactg atgctttgat tgttgctgtc ataagtcctg ttttgtcaca tacaccagca
     7621 cctatcatag ttactagaac tagacccaaa ggagcaaacc cttggaaatt gctgactatg
     7681 tttcctaaaa gaatttgaag ctgttctaca gtcaataaat ttacagcggt tatagtttct
     7741 ccagtaccag gatgaactac tgatataccc atagaaccaa ctagactaga tagtataagt
     7801 aaaagtacac ataaccctaa aaatattgta actgggtctg gtagtttatt accaacagtt
     7861 tctattctgt ttaatgtcct gtcaaaaaag tttttcttct taactacgtt atcattcata
     7921 atatcctccc taatgtttaa aaatataatt aaaaaataag tattgtggtt aatcttacaa
     7981 tataaattct gcttgtcgat ttaagacgtg tccacaatat gtcctaaaaa aagtataaaa
     8041 taaacttttt cctgtgtcaa tgcagttttc tgaattttct ggttgagtgg tgttagttaa
     8101 atatgcttta agtagattaa tgacgaattt tatctaatgt atttaaataa catatgcact
     8161 ttaaaattaa ttttaaaaca aaattttatg aaaagctagt ggaagattaa gttattaaaa
     8221 taagtagcgt agtaaagtgt tagatttttt aattaatcaa agaaatttac tttgtatttt
     8281 ctacaaaaat ctgtgaatat cttttgaatt atgtagagga tataaattta ttttatttac
     8341 atagattaga ttttgttcta atttactgca ttcaaatggt tttccatgtc caggataaat
     8401 cattttaggt tttgtattga tgattgtttt ccaagattga ataaattcta gtttgctttc
     8461 tgcaaatatg gtaattttgt gagtacttgg aaatccattc attgcagaat ctccacaaaa
     8521 taaaacacct tcatcaatta gtagtgaaat tgaatcagca gtatgtcctg gtgtatctat
     8581 aatttttcca tttagttctt tttcaatttc tttacgattc ttgtcagaca caagaataca
     8641 ccttttttca aattcttgtt taagtggaga aaaactatgc tcaccttttc caaatagttt
     8701 cataatttgg caaaagagaa aggcagtacg gctggtacaa ccaccaataa aagagttttg
     8761 acctttatat agaatttcta aagctttatc atgcataaca atctttgtat taggagattc
     8821 gtctagaatc tgatttagaa aaccaacatg gtcatcatga gcatgagtta gaaatatgta
     8881 tcgaatatct ttgattttta tgttgaattt agaaattcgt tttttgaaat gtttaaaacc
     8941 attttcatat ccagtatcaa tcaagacaaa gccatcttga atagggtaca accatgtatt
     9001 tataattcga tttcctgcat taaacatttt ttcaccacct tgaattagaa tagaaattta
     9061 tattaattag aaagaatata gcatagttct actgatattt caacgtagct ctaaaagttt
     9121 taaggaggat aatatatttt agtttaatct aaaaactaaa agaaaagaag tatactgtta
     9181 caaaaacaaa gagtggtata acttttacag tagataccac cctattataa aaatagactt
     9241 ataaagtttt atcgtgtttt tctaagaatg ttagatattc ttctttagtc ataactggag
     9301 taaaggtatc taatatatct ttagcatcct tagcattatc aaaaagaagg tctacaactg
     9361 taagtgccat tgctttagca ggaattatgt aagctaaatc ttcatcaact atttcaaaat
     9421 ctcttgtatg aagagctcct tttacaccac caatcatagg gtgaagagta ggcattatat
     9481 gagacacatc tccaaaatca aaagagcctg taaaatctcc accatcaata acttttcctt
     9541 ttagacctaa atcttcaaga ttgtttttaa ataaattatc aagacttttg tatcgtaata
     9601 ttggaagata tccaggaatt tcagttatct caacatcagc tccaactgcc attccaccag
     9661 ccatcaaagc cttatttatt ctttcattag catcaatcat accatttata gttctagctc
     9721 taacatatga ttccatatga acatcagcag gaacaacatt tactatatct ccaccttttg
     9781 tgatgatagg gtggaatcta actctttcag actctttaaa agtttctcta agtgcattca
     9841 cgttgtttat agcaagcatt gctgcattta aagcatttat tccatcatgt ggagcagaac
     9901 cagcatgaga ttctttacct ataaatttaa ccttttttcc aacaaatcca ttactctcag
     9961 gaccaactag agccttatct tctcctaaat cagaagcatg aaacatcata gatatgtcta
    10021 tatcatcaaa ataacctctt tttattaatt cttgttttcc accaaaataa gttatttttc
    10081 cttctttttt taattgctct ctgtattcca actcaataaa ctcttcagca ggagtagcca
    10141 taaaagatac tttaccatct aaatgttcta aaacgccact ttttactaaa ccaacagcag
    10201 cacctaacat tccagctatc tgaatattat gtccacaagt atgagaagca cctatttcat
    10261 tagcatcttt atgttcttta catgagatac catccaattc tccaagtata gatatatgag
    10321 gacctttctt gctagaattt gcatctgctt tgcatcctgt aactgctata tttttttcta
    10381 cagataaatt taattcttgc tcaaaaaagt ttgaaacagc ttctgtagtt ttaaattctt
    10441 tatatccata ttctggattt ttgtatattc cttgacctat ttttaatatt ttttctctat
    10501 tttcatctat agtttttatt actagagatt ttaattgttc tttattcata ataaattcct
    10561 cctatatttt accttgaata cttaaaacta tatgagctat actcgcacat gcaaagaata
    10621 tagttgtaaa tacaataagt gaaattataa ctattttcca tgacatttct tttaaagctt
    10681 ctattttgtt tccaacagaa attcctgcaa aagctagtag tggagtagtt atagccataa
    10741 agttaatatt attagtgtta gttaagaata cttcagatgt tggactatat ggcatactaa
    10801 gtaaaaggcc gattatagtt gcaaatccaa aagctggaaa gattgattta ggaaataaat
    10861 cttttataat catagctgcc atagcagcta atattagcat caccatacca ggaagagctg
    10921 taactataga tgtaccaacg ctaattcttt gtcctataag aattataact ccaataacag
    10981 acataaccaa taattgatat gaacttttct ttaaatttaa attagcaaac atttattagc
    11041 actctccttc tgtagattta atttctttag tttttttatt tctcgaaaat tttggttcta
    11101 aaatcttata cataaaatta gtaaatggta aggacacaaa aactaagaat ataattcctg
    11161 ttatactaga catcatattg cttgttgcag cataggcaag tattgtatct gccatttcag
    11221 gtggtaccat ggtagataat gaaccagcag ctgctgtcat catacttcca ctaccaacac
    11281 cacaggccat tcccaaagca tatgggtgaa gaccactata aatagatatt gaacctagta
    11341 cactaaagta tatagtacca agaactgttc ccattaaata tgtaccaagt acaccactac
    11401 cttctggtga gtttatccca tatttttctg atataactcc taaagaaggt tcacgactta
    11461 tacttgcagt agctccaaca gcttctcttt tcattccaaa cattaatgca agaggtaatg
    11521 caacgattgg tgcaagtata tgaccaaatt cttgagcgat aaacgctggt ccagcttgaa
    11581 ttattttgtc aatgtttggt ccaactgtag taccatattt tataccaagc attactagtg
    11641 caattccaac aacttcacca gcaatattta tttctttttc tccaaccact tttttaagcg
    11701 ctttaatttt tttacctaat aaatctggtg ttattataag tccaattata actgcataaa
    11761 gcatagagaa cagtactata gaggcttttc caatagctat agttctttgc cctataaatt
    11821 cacatattac taccaagatt aagcaagtta aaaatacttg aataaaatgt tttttattaa
    11881 tttccatgat ttcctcctca aaagaataaa tattttactt atctgaaatg agaaaatacg
    11941 ttttcttaag tataatacgt tttcctaaat aaaatgaaaa aactatttca aataagtttc
    12001 agaaatgtca ataacatgtc cttaattaag gataaaacaa aaatttaaca ttgtcaacga
    12061 aaaatatcta aaatattaca tattttgaat atattgtaat atagctttat tatgaaaaaa
    12121 ttgtttaaat attgtaggaa taatagctaa aaagctttaa aattagtaat ataaaattga
    12181 aaaataataa tatagaattg agaaatagta aattgaaatt ttaaaataaa tgagtggaag
    12241 tagcttagta aagtatggaa atgaata
//
LOCUS       DAKBNN010000117        12125 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000117 DAKBNN010000000
VERSION     DAKBNN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.118"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            537..1238
                     /locus_tag="OUB02_002566"
     CDS             537..1238
                     /locus_tag="OUB02_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HCT8886311.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            1264..1920
                     /locus_tag="OUB02_002567"
     CDS             1264..1920
                     /locus_tag="OUB02_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886312.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            2025..3665
                     /locus_tag="OUB02_002568"
     CDS             2025..3665
                     /locus_tag="OUB02_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HCT8886313.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            4023..5345
                     /locus_tag="OUB02_002569"
     CDS             4023..5345
                     /locus_tag="OUB02_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886314.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIGTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5542..6549
                     /locus_tag="OUB02_002570"
     CDS             5542..6549
                     /locus_tag="OUB02_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HCT8886315.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6587..7999
                     /locus_tag="OUB02_002571"
     CDS             6587..7999
                     /locus_tag="OUB02_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HCT8886316.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKGMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            8141..9406
                     /locus_tag="OUB02_002572"
     CDS             8141..9406
                     /locus_tag="OUB02_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8886317.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9448..11046
                     /locus_tag="OUB02_002573"
     CDS             9448..11046
                     /locus_tag="OUB02_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCT8886318.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            11081..12103
                     /locus_tag="OUB02_002574"
     CDS             11081..12103
                     /locus_tag="OUB02_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCT8886319.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4365 a         1369 c         2392 g         3999 t
ORIGIN      
        1 taagtattta atgttgagta gttagaataa caatgctttt ggattattta cttcatgtac
       61 agatttaaaa taagtttttt aaataatgag taaagataac agtatcttga ctaaaagtag
      121 gcttttaaga aaaaacaatg aaacttaaat gagaattaag agttagaaat gtcattattt
      181 tagatagaga tgaacataaa taaacttaaa aaatataaat tataaaaata ataatttttt
      241 ttaaaataat aaagtttttt gattaaaagt tttatattga aatgtcaaaa taataaaaga
      301 ccttataagt taattacagg ttaaaaaaat aatatgtgaa ttttctgata tttactaatt
      361 atgaataaaa tatttaagta ttaatgtttt tagtgagact ttaagacaga taatctccta
      421 ttaaagatac tatatcttag ataaaataaa ttaaaacaat ataaaaacat aacaagatta
      481 cagaaagaaa gctcacaata aaatgctgaa aaatttattt taaggaggaa taaagtatga
      541 agtttcaaga aatgattaat agcccaggtc tttggattgt ttctagtttt ttagtaatta
      601 tttcagttgt tcaagcaatc gtatttatga aagaggcact aaaagaggct tcaaatcttg
      661 gtattgaaag gaaaggaatt aaagcagcaa ttcgctcagc tagtgttaca gccattggac
      721 cttctctatc tcctgttatt acattattat cactggtagc tgtgattggc gctcctacga
      781 cttggatgag gctctgtgat gtcggagcag ctagaacaga gcttggagta atatctctta
      841 catccaattt atctggggta gaagtaggta gtgctgcatt tggagcagaa gctttttcgt
      901 atgcactatg ggggatggct ttaaataact taggttggct atttgttgta tttattttgg
      961 gtcatagaat gcgtggaatt gttgaaaaga tgaacatgaa gtataatcct acttgggtta
     1021 aaaaactact tgcaggagct acagttggat tatttgctta ccttctttca aatcaaataa
     1081 aaacatttga aataccaaaa cttacaccag caattatttc agctatagtt atgctagtat
     1141 tgacaacttt atttaaaaag aatcaacgat tacaagaatt atccttaggt attgccatgt
     1201 taattggtat gtttggtaca cagattatat tatcgtgatt aaaacggata agaggtgata
     1261 attatgtatg ataaaaagat gataaaaata gggagaattt ctataggtct tgcaattatt
     1321 gcaaacttta ttcccgctat ctatgtaggt attcgctttg gtcagatgcc aatgctttct
     1381 gttatatttc aaatttgggg gttagtcgct gcgacttatg gggttagttg gattgttcaa
     1441 cctatagctt attatcctac actaggagct ccaggttctt atataggttg gcttgcaggt
     1501 tctgttggag atatacgtat gcctgcagca tcaatggcac agaaaattgc aggtgtagaa
     1561 gcaggaactc atgagggtga agttgttgga acaattggta catgttgttc aatctttgta
     1621 tcagcttcta tgattaccat ttttaccatt gtaggctata aagtaattcc tttgttacct
     1681 gaatttgtta ctaattcatt taattatata cttccagcat tatttggagc aatttatgtt
     1741 gatattgcac gtaaggatat tcgagctggt ggatgtacga ttgctgtagc gttagcaatt
     1801 atgtactttg gtgaaaaaat tggtatacca ggtggattat taacattgtt tattgtgtta
     1861 ggtgggatac ttatcacaag ggtcttcttt gtaattgata acaataaaac aaaagaatag
     1921 gattgcttat taaggagaaa gactagaatt atttgttgag aaaaaagaat aggattgttt
     1981 attaagaaat atgaatataa ataagtcttg aaaggagaaa aagcatgttt gatgttaaaa
     2041 ttgtcaatgg aataatcgtt gatggtacag gaaattcaag atataaaagt gatgtaggga
     2101 ttgtaggaga caaaatcatg gctattggag atttaagtca aaaagaggct aaagagacta
     2161 tagacgcaac tggaaaattt gtatcacctg gttttataga tttccatact cattctgatt
     2221 tatctcttgt gtatgataaa tacacaagaa gccgtatcca tacaggtgta acaacagatg
     2281 ttattgcaaa ctgtggtata ggagttgctc cgataagaga agagaagaag caagagttaa
     2341 ttgattatct tggaactagg ataattggta caattccaac aaaattagag cttcattgga
     2401 atacaatgca agaatacttt gattacttaa ctgaaaattc tccagcagtc aatgttgtag
     2461 cttatgtagc acaaggacct gtacgtatca atgaaatggg attttcaaag gaaccagcta
     2521 ctttagagca acttaaaaat atgaagttgg aagttagaaa agctatggaa gctggatgtg
     2581 tagctctttc atcaggatta gtttatttac caggagcata cacaaagaaa gaagaaatgg
     2641 ctgaattatg taaggaattg ataccataca atggatacta tatatctcat attagagatg
     2701 aaggtgatga agaaatggaa gctcttgatg aagcaattta tatagctaag actgcaggag
     2761 ttcctctaca tgtttctcat ttaaaagtta tgggtcataa gaattttgga actattgatg
     2821 aggtatttaa aaagctagat gaagctgagg cagatggttt ggaagtaact tttgattgtt
     2881 acccatatac agctggaatg acatctttgg gtgcactttt acctccttgg gcttttgaag
     2941 gcggtgtaga aaatatggta aaacgattag aagtacaaga aaatagagac agaattataa
     3001 aagagttgga ggaagggatt cctggatggc aatgtttcta ccaattagca ggtgggtgga
     3061 acggtgttgt actagcctct gtaatgactg aagcaaataa atatgtagag ggtaaaactt
     3121 taatggaggt agccaaaatc aatggtgaaa atccatttga tacgttcttt aggcttttga
     3181 ttgaagaaaa aagtaaaatt caagtagttg ttcatacaca aggtcaagag gatacagata
     3241 aggttgtttg ccatccaaag tcatgtatag gttctgatag tatggacctt tctacagaag
     3301 gtttactttc tcttggaaag cctcatcctc gtgcatttgg tacttttgga cgaattttct
     3361 cttactatgt tagagaaaaa ggtatgttga cttttgagga agcagttaaa aagataactt
     3421 atctttcagc aaaacgctta ggcatttata aagaaagggg tctacttaaa gaaaattatt
     3481 ttgctgacat tgtggtattt gacccagata caatagaaga taaagcgact tatagtaatc
     3541 ctaaacagta tacagtggga gtggaatatg tgttggtgaa tggtaaaatt gcattggcag
     3601 gaggaaagca aactgatgta tgtgctggac gtgtaattaa aaatcctctt agcatagcaa
     3661 aatagaatac attaagaact tttataaaaa tagtctttct ttgaatatta aggaaggcta
     3721 tttttgttaa attaataaag attactctac ttgtgttttc taaagaattt aactttaagt
     3781 agaaaaaaca atatattaat tgtaaaatgt aattaatatt aatgaataag atttaaatac
     3841 aaaaatttaa atacaaaaat gtttcaacag atagtcgtaa ttaaaaaact taaagtaact
     3901 ttttatatta ggtataattg attattcaaa attctatgaa tcaggagtcc tgtgccttta
     3961 gtcatggtag gtttagaaaa tgttttaaga gataatagta tcattaggaa aggagagacg
     4021 aaatgaaaat actttatgta ggaccaggta tggtaatcga taaaatacta gcagtaagta
     4081 aaaagaattt tcctaatatt gaagcagatt ttcttagata tgatagatat attgaagtac
     4141 ctgatttatt ggaaaaatat agaggtagag tggatgcagt tctttttaca ggtaagtcgc
     4201 catttaaatt ttttgaaaaa agacaaaaag attttgtatt gtctgattat attcctagac
     4261 atgaaacaac attgtacaga gtgttgcttg aaattacata tttgcttaaa ttcgatatta
     4321 gtagacttag tataggtact tatgataagg ctatgatgaa gagattgtat aaagaaattg
     4381 gagttgaatt taaggatgaa catatgtttt ttgcagagca aaagtattta gaagaggact
     4441 atataaagtt tgtgcttgaa tttcataaat ataattatca aaataatgat gtatgttgtt
     4501 gtattacagg tcttgaagag gcctatcata taatgaaaaa agataatatt ccagtagttt
     4561 tagccttgcc ttctgaggac gtaatggtgc aaagtataaa aaacattcag atgcgttata
     4621 ttgcaaagaa aaacaatgag aatcagatta ttgttcttgc tattaaactg aacatgcctt
     4681 cagaatattc actaatgaag gaagatgagt acgcttatct ttcacaaagg attaagattc
     4741 ttgataaact ataccatttc aatagccgta tagatggtgt tttggtagag cagagtagaa
     4801 gtgaatttat gatttttaca actaaaaaaa tacttgagtt agaaacaaag aaatacagag
     4861 atatttattt gttggatatg attcgtgaag tttcagttat aaacacttat ataggaattg
     4921 gttatggaaa aacagcaaat gaagctaaat ttaacgctta tgagagtatt aagatggctc
     4981 aaagtcaaaa aaatgatgca gcatatgtag tttttgaaaa tggagaaatt atgggaccac
     5041 taaagccaaa tcctactgtc aaagagaaaa atagttttga tgaacgtttt tatcaaattg
     5101 ctacagagac agggctaagt gctaatactg tatataaagt attcaataaa ataataaata
     5161 gtggaaaaac tgaatttaca tcaaaggaat tagctactat atgtggtgta agtattagaa
     5221 ctatggacag gattatactt agattgtgtg attctggtta ttgtgaggta gtgagtgaac
     5281 agcttatgag caagtatggt agacccagta gaatattacg ttttaaacct tttctgttgt
     5341 tttaaataga tataagtcaa aaattaaatt tttatgataa cattttcttt acaacttttt
     5401 agatatatgt taatatttag aaaaatgcat atgtattcat aaaataaact caaaacagct
     5461 aaaaaatcat taaaaaggaa atcgtatgca taaaatgaga aagttaattt ataacttaaa
     5521 taaatctggg aggagataat tatgggaaaa tatcaggatt caaaaaaagt tgtaagaaat
     5581 tattttgaag cattagaaaa tgctactggg gatgaggttg aaaatgtctt aaagcaacat
     5641 atgaaatcag attataactg gaaaggtgtg tatccatttc gtgaacaaga aggcactgaa
     5701 aatgtagcag atgtattttg gaagccttta aaaaaatcat tgagtaatat gcaaagacga
     5761 caagatattt tcattgcagg aacaaatgag attgacaata gtgaatgggt aatgagtatg
     5821 gggcatttta tgggactttt tgataaggat tggcttggca ttaaacatac tcgcaaaatg
     5881 attagtttaa gatatgcaga atttaactgt gttcaagatg gtaaaattgc taaaactgga
     5941 ttatttgtag atataattgg gtttatgatt caagcaggag ttaatccatt gccaccacaa
     6001 acaggttcat attttgtata tccagggcca attgaccata atggattact ttttgaagat
     6061 gcagatgaag ttgaaggagt aaaaacttta gcactagtaa ataaaatggt agatgattta
     6121 tctgaattaa atgaaagtgg tgctatggga tgcccaccag aaatacttga aaaatcttgg
     6181 gctaaagata tgatttggta tggtccaggt ggaattggtg cttcttatac tatacctaga
     6241 tatcaagagc aacaccagct accatttaga aataatttaa aaggaaagac ttttaatggg
     6301 catgtatgta gatttgcaga aggtaatttt gcttgtttct ttggatggcc taatctgact
     6361 aatgtaccat gtggaggttt tttaggatta ccaggtggag aggttaaagc agatatgcaa
     6421 gttgttgatg tttattatcg taagggcgat aaacttcaag aaaactgggt gttaattgat
     6481 attccttatt ggttgaaaca acaagggctt gatattcttg agagaactaa aagtattttt
     6541 aatgcttagt tatgcatacg tattctaaaa caaaggggga tataaagtgg catctaccaa
     6601 caaaaataat gcaacaagta aagaggaaga aaaagaagtt tctacaagtg ggcttgaatc
     6661 atgtacggtt aaagaaaaca aagctaaaat gaatcaaact aattttagta atataaatga
     6721 aaatcaaaca aatgagaata aaacgaacac atataaagga aatatagata aagggaatat
     6781 agataaaaca aacacaaata aatccgacat aaataaagtt aacatagata cagtggatac
     6841 agataaatta aatacaaata ctcttactaa aaaaaataaa aaagttatat ttaaggggga
     6901 agataatatg tcatctacaa aaatttacta tggagattca ttagaagtaa cgcctgttgg
     6961 agtaatggat tacaatgatt tttcaaaaca gacaaaaagg gaacaggaaa tacctggatt
     7021 tgattctaag tatagagatt ttgtagatta tattatgaag attacacata atatctggga
     7081 agaaaaaggt ataggtgtta tttatgatac atatcacaat aatgttacaa tgcattgtgg
     7141 ctcttcaaat ttagttggta ttaaagatgt tatttccaat actcttcaaa cattacatgc
     7201 gtttcctgat agaagattaa ttggtcaaaa tgtaatatgg tctaactttg gtgctgatgg
     7261 ttttttatct tctcaccgtg ttttgtctac agcaactaac ttaggtgata gtaattttgg
     7321 accagcaaca ggtagaaaaa taaactttag aactgtaatt gattgtgcca caactaacaa
     7381 tagaatccac gaagaatggt tggttagaga taatttatgg attgttactc aattaggatt
     7441 aaaccctcag gaagtagcaa agggtatggc aaaggcaagt gaatctaaag tgcttagttt
     7501 acaatcaact tatggtatct gtgaatctat ggatggtcaa tttatgccaa ctaagtatca
     7561 agcaaaagat gattctgttg gcgaaatgat gcttgaaatg acaagccgca tttataatta
     7621 taagtatata aatgaagtta aaaaatacta tcatgataat gcagtagtac attttatatg
     7681 tgataaagat ttaaatggct atgatgagat tcaaggaatg attgtaagtt tacttgcatc
     7741 aattccaaat ggaagttatg aagtggaaag agtaacttgt aatcaaagag aaaaaaatga
     7801 aggttatgat gtatctgtaa gatggagatt acgtggaata aatgaaggta taggattttt
     7861 tggacagcct tctggaaagc atatagaagt aatgggaatc aatcattatc atatactaca
     7921 aggtaaagta aaggaggagt ggataacttt tgatggtatg gatgtattaa aacaaatgta
     7981 tatgggagta gaagaataaa gctgtttaga gtataaatag ttttgaaata tatagggaag
     8041 tattgcttta taaaatttaa gtggggaatg tatatgaaaa atagatgttt aaacttttag
     8101 caaaattatg actaaattta aaagggggaa ttataaaaat atgaaaacaa cggttgaaat
     8161 agaaaatgat atcatattag gaaaagacta ttcaaaccta aaaagaggaa ttgcattttt
     8221 ttcaattgct ttaatatact ttttttattg ctacaatttt atggtgggta cttttattaa
     8281 accaacaatg atttatgctt tagcagatgg gggatttggt ttttcattaa aacaaacaga
     8341 agaaatattt gcagttatgt cttttggtac aattccaggt acatttattt ttggggtgat
     8401 ttctacaaaa attgggaaga agaggacttt aattagcgtt gctttactga ttggattgac
     8461 aacatttata ccaatgttat ctcctacaaa tattatgtta tggaaaatag cacgtttgtg
     8521 tacaggagta gtgttgggag gcgtatttgg aacagctatg cctttagttg cagatatgtt
     8581 tccaagtaaa tatcgtggta agttagctgc tatattaact gctttatttt cattagcaat
     8641 gatttttggt gggaaagtat atggcgtttt aggtgatgca aattggcaaa tattgatgta
     8701 tactgcgatt ataccaccta tagttggagc tatcttagca atcttctttg tgccagatga
     8761 tttggagtat acaaaggaat tagttaaaaa gggaaaagaa actggtgaaa agattagcta
     8821 tataagtatg tataagggaa aatacttatg gattggattg ggaacgattc ttttatcagg
     8881 tgcaaatttt acagcatatt cagcattttc aaataatgca actacatacc tagtgacagg
     8941 aataggaatg tcagcagcag tggctgggtc tatatatagt ttacaaggaa ttggtcaatt
     9001 agtaggatat ttattctggg gttcaattgc agataaattt ggaagaaaaa taccttttat
     9061 aggtatggca ttagcagcag tgtttgtttt tatatataca aaattaggtg gagataatac
     9121 aacaacattc tatatgatat cagtattact aggtgttgct gttggatatt ctggagcatg
     9181 gggagcatac tacacagagt tgttcccaag taaatatcgt tcgttgtcat ctggtatgtc
     9241 atttaatgga ggaaggatta tatctacgtt tgcaatacct gctatagctg gtacagcttc
     9301 tggtacttta ggtatgatga ctatctttta catatcaatc gcagtctttg tagcaggtgc
     9361 tattgtatgg ttattcttac cagagacgtt aaataataaa aaataaaata ctaggaattt
     9421 attttgttac ttagaaaggg gtggcttatg atttcatcaa ttgatgtatg tattataata
     9481 ggatatttgc tattaatgtt agttattggt tattattcag gaaaagataa taaaaatcaa
     9541 gaagattatt tcttggcagg taggtcaatg ccatggattc caattgcttt atcagttgca
     9601 gctactatga ttagtgcaaa tggctttata ggtggtcctg gatgggcata tgtagatgga
     9661 atgtatccag taatggttaa tattacagta cctttagcaa tattttttgc actgagcatt
     9721 accaccccag ttatctataa attaaaaata acctctatat atgaatatat gggacttcgt
     9781 ttaggaaatt attcaagagg attaactatt attcaatttt ttattaattc attgattcaa
     9841 gctagttcta tggtatatat tccagtgctt attattcaaa tgattactgg ctggtcactt
     9901 tatgctttag tcccaatagt tgtacttgta tcaattatat atactctatt aggtgggatt
     9961 aaggcagtta tatggactga tagtattcaa atgatagtag taataagtgc tgtatttata
    10021 gtaattattg tagccttaaa aggaatgaat ctaagtttct ttgatacatt acaaattgca
    10081 caacagagtg gaaaattaaa tacatttgat ttcagtaggg atatttcagt gacaaatact
    10141 ttttgggcta ctttaattgg tggaactgta atgtggataa gatacttttg ttttgaccaa
    10201 tctcaagtac aaagagtttt aacatctaaa tctttaaaat gtgcaaaaaa ttcttttgta
    10261 gttagtgcat ttgttatgaa tttagtttac tactttatgc tattggtagg agtaatttta
    10321 tttgtttttt atgggggaag gacatttgaa acttcaaatg aaataatgat tacttttatt
    10381 ttaaatgaac ttccagtagg agccattgga attattattg caggtgtgtt tgcagcagca
    10441 atgtctagta tcgactctat attaaacagt atgacaactg tttttacgaa ggatatttat
    10501 gagttttatt tcaaaaaaga taacaaagaa tcatcattaa aagttacaat gattataact
    10561 gtagttatag gagttgttat gacaggtttt attattatgg gatttggagg aagcatcaaa
    10621 tcaattcttg accttgttgg taactatatc tcatactttg ctggaccagc tacaggtgca
    10681 tttgtactag caatgtttac atataaagct aatgataaag gagtatctat tggttttatt
    10741 gtagggttag ttttaggatt ttttatttct aataaatata atgtgagttg gttgtggaat
    10801 ccagcaatag gtgctacaat aacattgata tttggatata tttttagtgt cattttaaag
    10861 aaaaatcata atgcagatga tattaaaaaa tacactgcat ttggtatgag aaatcacctt
    10921 atttctcaag gaatggataa agtagatgga gtatctatag tgccattttc tttaggtaaa
    10981 caagaactaa ttgtattagc tttttttatt atccaatata ttatattatt tttgatacaa
    11041 ttttaagaag aaatactaga tatttaagga gaaatactaa atgaatcaaa agaaagttca
    11101 ttataataaa tcggctacct caaaagatgt tgcaagattg gcaggagtat ctcaatcatc
    11161 tgtttcaaga gcatttggta gtgcaaatgg aaagggcgta aaacctgaag taagagaaag
    11221 aatttttcgt gtagcaaacg aaataggata tgttcctaat ttggtagcac ggggaatgat
    11281 tagtggaaaa actaatgtta taggattgat agttggtgat agtctaggtc cattttacaa
    11341 tagaattatc aatttatttg tagaaaaaat tcaagagata ggtaaacagt gtctcgtttt
    11401 taaagttcct agacaacaaa gtatagatag tataatttca aaagtaattc agtttcaagt
    11461 agaagctgtg attattacag cttcggccat gaataaggtg atggctgaga cttgtgaaga
    11521 aaacaatatt cctgtgattc tttttaatcg ttttatacct ggtataaaaa taagtacagt
    11581 ctatgtagac ccaattgaag gtgggggaat ggctgctgaa tatctgctta agaaaggtca
    11641 taagaatata ggatatattc aatttacgag agaaacaagt gaagaaatgg aaaagaaaat
    11701 tggattttat tctaagttaa gacaaagtgg gattcataat cttaaagaag aatctgcaaa
    11761 ctatgattat gattctggat atgaagcagg taagagaatg ttagcattat caaatcctcc
    11821 aacagcaatt ttttgcacaa gtgacttgat agctattggt gtaatggatg ctgcaaggtt
    11881 tgagtacaag ttgagggtgc cagaggattt atcagtgata ggatatgatg atattcaaat
    11941 ggcttcatgg aagagttata atttaacgac gattagacag ccactagatt tgttgattga
    12001 aaaaacaatt aacatactta agtccttatt aaatgaagaa aatccagagt cagtcgttga
    12061 aatgttaaaa cctgaattga ttgaaagagg ttctacactt taagtggtag atatttaatt
    12121 aagtg
//
LOCUS       DAKBNN010000118        12084 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000118 DAKBNN010000000
VERSION     DAKBNN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.119"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..685)
                     /locus_tag="OUB02_002575"
     CDS             complement(<1..685)
                     /locus_tag="OUB02_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8886320.1"
                     /translation="MRLEEMIFFNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYK"
     gene            complement(852..2051)
                     /locus_tag="OUB02_002576"
     CDS             complement(852..2051)
                     /locus_tag="OUB02_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HCT8886321.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDIKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(2574..3911)
                     /gene="xylA"
                     /locus_tag="OUB02_002577"
     CDS             complement(2574..3911)
                     /gene="xylA"
                     /locus_tag="OUB02_002577"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCT8886322.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(4007..5557)
                     /gene="xylB"
                     /locus_tag="OUB02_002578"
     CDS             complement(4007..5557)
                     /gene="xylB"
                     /locus_tag="OUB02_002578"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCT8886323.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            5765..6925
                     /locus_tag="OUB02_002579"
     CDS             5765..6925
                     /locus_tag="OUB02_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HCT8886324.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDVGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(7081..7485)
                     /locus_tag="OUB02_002580"
     CDS             complement(7081..7485)
                     /locus_tag="OUB02_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8886325.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(7506..7988)
                     /locus_tag="OUB02_002581"
     CDS             complement(7506..7988)
                     /locus_tag="OUB02_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8886326.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(8008..8817)
                     /locus_tag="OUB02_002582"
     CDS             complement(8008..8817)
                     /locus_tag="OUB02_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCT8886327.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(8810..9589)
                     /locus_tag="OUB02_002583"
     CDS             complement(8810..9589)
                     /locus_tag="OUB02_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCT8886328.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(9589..11667)
                     /locus_tag="OUB02_002584"
     CDS             complement(9589..11667)
                     /locus_tag="OUB02_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCT8886329.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT         3917 a         2164 c         1314 g         4689 t
ORIGIN      
        1 atttataact agggtgcatt tgtaaaaatg agatattagt cgctgaaata taaattgatt
       61 catcacttaa atcagtcaat gtattatttt taaactctgt aattgaaatt attttagttc
      121 ctattaattt taatcttcta gcaatattaa gtgtattctc attctcacca cttagggata
      181 taataatcat aacatcattt ttactgacaa tattgtaaaa agaatcatca aatgttcttc
      241 cctcaaaatc atatattatt tcttgacatg ataagaacat tctctttatc tgttttgcaa
      301 ccacattttg aatatcacct gaaccataag cgtatattgt tcttgattca taaagaagtt
      361 tacacaatgc atcataatct ttattttttt gatcttcaac atacttaatt attgaattac
      421 atgcaatatc aaatgactga ttttctatga catgaacctt ttctgattcc cagcttaata
      481 atactttaaa ctcactgaaa ccttttaaat ctaatttttt aacaaatctt gtaatagttg
      541 cacgagacac attacatttc atagcaagtt catttattga catcttacta caatgttttt
      601 tatttgcagt tatatatttc catattaata aatctgtatc attcaattta ttataattaa
      661 aaaaaatcat ttcttctaat ctcataaact attaccccct ttaattaaaa atcgtcataa
      721 atattatacc aatattcaac attaaattac agcgtattct tatatttact atttagccca
      781 taaaattgac tataaaaaat actgctataa ttgatgttta aaaaaatgta gttttaattt
      841 tattctttta tctaataatt aatattttca agttctagtg cttttcttat acaattatca
      901 tctttaaaat cagcagtcct tttacaatct tttactttaa attctggaat agttttaaat
      961 tcctcatcac atattccatt cttatttaaa tacatacaac ttgccattct aactattaga
     1021 ccactatttt ttaaattaaa caatacaggg tctatacacc ctccatctcc gccagtagtt
     1081 tcaccaacta aagtcgcaaa tttattttct ttacaaaaca ttgcaaacga ctctgatgat
     1141 gaatatattt ttctatcaac aagtaagtat atattaccct taaattttga atcatctgta
     1201 gattttatag tataggaagt atcttcaaaa tcattaaaat cttttataat ttctttaggt
     1261 gcattttcct ttacacttgc tggtaaattt tctacaggat ttaattttat atttcttgca
     1321 tttgtatatt tttttactat ttcattatta tttctataaa ttctataacc atttattttg
     1381 atatcatttt ttatgagttt agatacgatt ccctgccaat acctatcact tccacctaaa
     1441 ttacctctta tatcaataat taaagcttta tgtttttcta aagtgttaat ataatctcca
     1501 attattttta aatcattatt tatagagcca ttctttgatg acattgatgg taaatacata
     1561 tatccaatct gacccttaat aacatccttt aaaattaatt ctttttttac aaaaatatca
     1621 tctggcattt ttattttaag attcatagag tcatatctat ccacaacttt tttatcatta
     1681 agaaaatcat accaatcatt attagagtat gatttcttaa aaagttcata tcttttctta
     1741 ttatctatta cttctgtgtg tttattattt aaatcagata atatagatga cagttcttct
     1801 ataaattctt catcattttc agtgtttttt attcgttttg tatattcatc cttatttgct
     1861 aaccaatcaa tattattaag cctcttattt acacctagat atggatatcc tgtctttaca
     1921 acattataca cataattaaa gtcatccacc ttttcctctt cagacaactg attcttttga
     1981 gaataagtag aattacctaa taagattata aatgctaaaa taattactaa aaataatact
     2041 ttctttttca tgtaattctc atccactttc agattttata ttttaataca ttgtatcatt
     2101 attttattat cacctaaaca gttaaaagaa attcttgaag atatattcca ctattattct
     2161 atttaaaaat ttttatttgt attttcaaaa ttatttcatg aaagttataa aataacaatt
     2221 atattcaaat atctatttta ctgctatatc ctgaaagtaa attcttatca gtgatatact
     2281 aaaaaagata tgctaaaaaa tttatccatt ataatgactt aaatttagat actttactga
     2341 tttttattta ttagttgaga aagatattga taattaaaaa tattgataac tatagaaatg
     2401 attatttttt atagaaattt taaggatata aactaattat acttaagtct atatccttaa
     2461 tttatcatga ttatgagaat ttttacaaat acttatcttt gtccgtttta gaataaaaac
     2521 aagaacatat cctacttatt ttattcaaaa atccaagttt aattactaca caattactta
     2581 ttagaaattg tatctacttc atatatatat ctgttaagta tagcttctag catttcttgt
     2641 cttccagact tattctttat aactggcatt tgcagtgcat aagattctaa ctctttaaat
     2701 ccaaccttac cttctactat atccttacct ataccttcac taaaacttgc atatctttct
     2761 ttagtaaagt tttcaaatac ctcatcttct aataatttat gagctattaa taatcccttt
     2821 gcaaatgtat ccattcctgc aatataagct aggaataagt cttctacctc aaaagatgct
     2881 cttcttactt tagagtcaaa gttaaatcct cctggtgcta ttccaccttg ctttaacact
     2941 tcatacatag caagtgttgc atcatatata tttgttggaa attggtctgt atcccaacct
     3001 aataataggt ctccttgatt tgcatctata ctacctaaaa cattgtttat tcttgctata
     3061 tttaattcat gttggaaagt atgtcctgca agtgttgcat ggtttgcttc tatattcatt
     3121 ttgaagtatt tatccagatt atactttctt aaaaatccta aaacagtagc agtatcaaaa
     3181 tcgtattgat gtttagtagg ctcttttggc ttaggctcta ttaagaattg gccagtgaat
     3241 cctatttcct tagcataatc tacagccatt tgtaagaatc ttgcaaagtt atccatttca
     3301 agttctgtat tagtgttaag taaagtttca taaccttctc ttccacccca gaatacaaaa
     3361 ttttctcctc ctaagtactt agttatttcc atagcttttt taacctgagc tgctgcatat
     3421 gcatatacat ctgcattaca tgttgtagat gcaccatgaa caaatcttgg atttccaaac
     3481 atattggcag ttccccataa taactttata cctgtctttt ccatattaac ttttatatac
     3541 tcaactattt catctaatat cttatttgtc tcttctaaat ctcttccttc tggtgctata
     3601 tctctgtcat gaaaacagaa atactcaatt cccatcttag acataaattc aaatcctgca
     3661 tcaactttaa tctttgctat ctcaattggg tctgttatat tattccaagg tctctctact
     3721 gtccctactc caaatgggtc attaccctct ccacataaag tatgccaata gctcattgca
     3781 aatcttaagt gttctttcat agtcttatta cctaccactt gttcaggatt gtagtatcta
     3841 aatgctaatt cattgtcaga ttttactcct tcaaatttta tctgtcctat tcctttaaat
     3901 atttcactca tttcatgtcc tcctaatatt ttacacatat aatatatgta gtatgttttc
     3961 ttaatttaaa actattttct atctttattt ttctttttaa gctaatttaa ttatttattt
     4021 ttgcattaac tacttcatct aataacttaa agtcatcttt caaagatttg tatattcttt
     4081 tatacaatct atgatatata ttgtatgtat ctacattttc acttattggt tctaatttag
     4141 ttgaagtttt tattatagca tcacatgctt tttgtatatt ttcatatatt ccagctccta
     4201 ctaatgcaag aattgcaact ccaagtgctg gtccttcact tgaatttata gtatatacat
     4261 cttgattaaa catatctgct tgcatctgtc tccatacttt actttttcca cctccacctg
     4321 aggctcttac ttcattaacg tctatattaa gattctctat tatatccata cagtctttaa
     4381 gactatatga aacaccttcc attattgacc ttgcgatttc ttttttagaa tggattggag
     4441 ataatccaaa gaacacacct cttgcataag ggtcaagatg aggggttctt tctcccatca
     4501 tatatggtaa gtaaagcaat ccattacatc ctgttggtac ttgacttact tgattattta
     4561 taatctcata tacatcttca cctaaagaat ttgaaacttc cacttcactt tgacagaaat
     4621 tatctttaaa ccattttaat gacaatccag ctccttgagt aactcccata acatgccatg
     4681 tatttggtat tgcatgacag aaagtatgaa ctctaccttc tttatcaatt tttggctctt
     4741 ttgtataagc aaatactaca cctgatgtac ctatagtaga tgaaactact ccatctttaa
     4801 ctattccatt tcctatagct cctgctgctt ggtctccagc tcctcctacc actattgttc
     4861 cttctttaag ccctgtcaaa cttgctacat ctctagtcac ataacctgta atctcttgtg
     4921 attcataaac tttacctaaa agatttttat ctatatttaa cttttctagt acttcatcgc
     4981 tccaatctct ttttcttata tcgagcaact gcataccact tgcatctgat acctctgtag
     5041 catattcacc tgtcaatttg tatcttatat aatcttttgg aagaagtatt ttattagtat
     5101 ttttatatat atctggttca ttgtttctaa cccataatat ctttgatgct gtaaatcctg
     5161 taagtgctgg attagcagtt atttcaatca gtctttcttc acctactaaa tcagttatct
     5221 cctcacactc ttttactgtt cgttggtcac accatattat agagtttctc aatgttttac
     5281 cttcattgtc cagcataact agaccatgca tctgtcctga taatccaatt ccttttatac
     5341 attcactgct tatatttgaa ctttgaacta cttctcttat agattcgact acagctttcc
     5401 accaatcatt tgcatcctgc tctgcccaac ctgatttctc ttgtataaga ggatattcat
     5461 atgtacaact ttttatagta tttcctagtt tatcaaataa aactgtttta gtacctgatg
     5521 taccaatatc cacacctaat acatactcca acttcatccc tccttgaatg ccacttcata
     5581 actatcgttt tcctatccta atttttttat attttaggta tattttactt gctttatttt
     5641 tgttagtata ttaaactaac ttatgaacta ataataaaat atttaattat ctttgtcaat
     5701 gcaaaataac cttttatttt tgtgtaaaaa gtattacaat attattttaa ggaggaatat
     5761 caaattggtt actgataaat acactattcg agaaatgaat gaaagattgg tacttgaaca
     5821 aattataaaa aatggtccta tttctagggc aagtatagct tctactatag gattgaataa
     5881 agcaactata tccgctatta ccaaaaaact tattgatgaa tctctagttc atgaaatagg
     5941 aattggaaat agtactcata gtggcggaag aaaacccata ttattagtat ttaataaatg
     6001 tgcagggatt tctttaagta tggacgttgg atacgactat atattttctt ctctatctta
     6061 cttagatgga actattataa attctaagaa attaacagat atacaagtaa gtaaagacaa
     6121 tgttatacaa ttgatagatg aaattataaa tagttataac atatccaaaa tagacactcc
     6181 ttataaggta attggtctta ctttggctat acatgggatt acttgtgaaa ataaagtatt
     6241 gtttactcca tattacaatc ttaatgagat tgacttatat agcatcctta gcaaaaagta
     6301 tgactttcct attcatatag aaaatgaagc aaacttaaca gctttagctg agaatacttt
     6361 ttcaactgta cataactcac tacttagtct cagtattcac agtggatttg gttcaggcat
     6421 tataataaat aataaattat atagtggcag aaatggtatg agtggagaaa ttggtcacac
     6481 tatcatcatg cctaatggta aactatgtcc ttgtggaaat cgtggctgtt tagaacagta
     6541 ttgttcagaa aaaaaggtat ttgaacagct ttcttcttta gaaaatattc ctaaaataga
     6601 ttctgatata gtaaagcaac tatattatga agataaccag aatgccaaaa aagttattca
     6661 tgagttttgt agttacttaa caatagctat aaataatgct attacaactt atgctcctga
     6721 aataatatat ttaaatagcc aaataattag tgatatccct gagatattac agattacaaa
     6781 agatatgtta gttagctctt ttaacaaagg aataaatata gaaatttcat cattagggtc
     6841 tgaggcatct ctatatggtg gctctgccgt aaatatcaag agctttttga atatacaaaa
     6901 tttaacttta attaatgaaa tataagggtt atgactatat acagccataa ccctttaaat
     6961 tatacattct aaaattagat ttattatata gtttatgcaa gattagctat attttttaaa
     7021 aattgtatat caattaaata ttaacataag tatttcgttt taaatattaa tataatcttc
     7081 ctactcatca tcagataata aacttgcatt ttgttcaacc atagaaatat tatctttacc
     7141 tattttctct aattttgctg ccaattcctt tgtatctaaa aaagctgatt caataaccat
     7201 acctaaattt agccctgaaa taattttaat tcgactgtca ttattggcta ctaataaagc
     7261 agcattacat ggagttccac ctagtaaatc tgccattata attatttctg aactttcctt
     7321 gtacacttct aaagcttttt ttaatttttc tattgttcct tctattccat catcttgttt
     7381 catatttaca gtaggataat ctatagaatc ccctattatc atttttgcag aatttaagac
     7441 ttcttcagcc atattgccat gactcatcaa aataatacta ttcaatttaa tacacctctt
     7501 taaatctata agatgtctac aagataagtt ttcttatcat caggcgtcat tatagttgta
     7561 atttcaattc cttcatctaa aagtctttta aatgcttctt tctcttcttc agtaacactg
     7621 atattaggtc ttagaactgt tgtatctttt ctacttgaca tatttcctac atttatatta
     7681 tgtattttaa atcctgcctc tacaaatgga ataaggtcca caggagactt aacaattaat
     7741 aatacatttt gtccttcata ctttcctgca gtaatatttt ttatagcagt ctcttctgta
     7801 attatagatg acgctatacc tgcaggtgta gccattctta aaacttgttt ttgaacagaa
     7861 ttgttagcta ctgcatcatt tactaccatt aatcttgtaa cacccaattt attactccac
     7921 atagttgcaa cttgaccatg gattaaacgg tcatctatac gaatattttt aatacctttc
     7981 atttccatat ctaatatcat cctttcctta ttttaaaata cctaatgcat ataaaattat
     8041 tgaaacaacc attactccta aaattaagta tataggtttt atattctttt taagagccca
     8101 ataaacaatc acaactagaa ttgcaggaac taacccaggc ataatttggt ctaatatttc
     8161 ttgacctttc attgtaaaat caccttgttg aaagttcaaa gcaacattag cttttacaac
     8221 tgaaggtatt aatgcaccta caaccattaa acctaaaata gatgtactct cagttaaaat
     8281 ctttaaactt ttttcaattg aagatattag cttagtacct gatgtataac cagcattaaa
     8341 tagtggtata cttaataatt tcaatgctac actcactaca atccacagta agattccaaa
     8401 cggacttcca tctatcgcca tattagctgc tatagctcca aaaattgtcc acggaataac
     8461 gacaaataaa ctatctccta aacctgctac tggtcccatc aagcctgttt taattgaagc
     8521 aacagcctct tcagtctctt cacttgataa ctcctccatt gctacattaa tacccaaaat
     8581 ataaggggca acccaaggat tagtgttaaa aaattgtaaa tgatttaata cagcactttg
     8641 taacttttct ggattatctt tgtatactac ttttaaaaaa ggtagtactg cataacagta
     8701 tgccaaggcc tgcatcttct cataatttga agaaacagaa ctagtaaaaa accagcgcca
     8761 taagacactt tttttcacct tttcatttga tttatttaat gataccttat cactcatctt
     8821 cgtccatacc tcctactaca gtatattgtt gttcttgatt ttccaaattc tttttgtaaa
     8881 ttataagtgc aattgctcca ccaattaaag ctacacctaa taatggtact ttcatatata
     8941 ctgctaaaac aaaaccaatt actaagtaac taaaatactc cttagcagga agatatctaa
     9001 gtaataatcc tatccctact gctggaagta gtcctccagc tactgttaaa ccacctgtta
     9061 accattcagg tgtatatttc aaaatggtat taattaaaga aggtccaaat attacagata
     9121 gagcaactgg aatacctgtt gtagctgctg ttaatagtac tcctaacatt tgcatatagg
     9181 ttatattttt ataatttcct tttttagctc catcttctgc tttatgaact agccatatac
     9241 caatagtatt tctaagaaca tctaattgta ccatcaataa agcaactgga atacctatag
     9301 agattcctac agatgcttct tgcttagtcg taatagttat aaatgtggct actatacttg
     9361 ctgtttgata atcaggtatt gatgctccac caaagttact aatacctaat gataatagct
     9421 gtaatgtacc tccaatatat aatcctgtat ttatatctcc taagataaga ccagtaataa
     9481 aacctgctgt tactggttga tatgttccaa acattgttga atttttttca tagtttataa
     9541 aaaatccata tagtacgatt aataataatt gccataattg tggctccatt atgatttcac
     9601 ctctcccaaa atatttttta gttctttaaa attataactg gaatcttgct ttaggaaaat
     9661 aggaatttct tccagtgggc attcaacttc ataagttttc ccaccttcat aactaactcc
     9721 actaaatata ttttcccatg tatgtccttt tggtaaatat acttttctac ttctatcttt
     9781 ataattcatt attggagcta ctaaaatttc atctccaagc atatatgtat tatccacctc
     9841 ccaagtttta tcatcttcag gaaattcata aaataaagtt ctcataactg gtgttccatc
     9901 ttcatgtgct tctttcatca gtttttttaa ataagttttt aagctctctc taatttttat
     9961 gaattttgta agaattactt caacttcttc accataagac catatttcat taggagctcc
    10021 agttaccatt gagcctccac ctttatttga taatggttta ctatgaggca atctatctcc
    10081 atgcattctc aaaattggtg aaaaagtagc atattggaac catcttacca taagttctct
    10141 aaattctggg tctttaggat ttcctccatg gaaacctcca atatcagtag tccaccatgg
    10201 tatacctgcc agtcccatat ttaaccctgt atttacttga ttattaaaag cttcaaaact
    10261 agaatcaatg tcaccagacc atactaaagc tccatacttt tgagctcctg cccaagctcc
    10321 tcttacaaga gttacaacag actctatgtc attatgtaaa ccatcataaa tcatttttaa
    10381 ataatcaata ggataaatat taccacaact taaaacattt ccttttttat atctatagtt
    10441 atcaaaatca tacacagaat agccaggttc agctacatct aaccaataat agtcaatccc
    10501 aagttgttta taatttttat ctattctatc ccaaacatat tctcttgcat tttcatttgt
    10561 catatctaca aagactgtat ttccttgaat ttgcattgtc attctaacac cacgattaac
    10621 attgactaaa tagccatttt ctaaataatc attgtagttt tctgcatcac tttgaactgt
    10681 aggccataca gatactattg gttctactga atactcttct ttcaattttt taactaattc
    10741 tttaggattt ttccaataat ctaagtcaaa tttatattca ccttgcttag gccaatgaaa
    10801 atagtcaatt gcaattgatg aaagcttaat tcctctttta gaatattcct tcacaacatc
    10861 taaaacttct tctgaagttc tatatcttag tttactttgc catagtccta aaaggttttc
    10921 aggcatcata ggaacagttc cagtaacttg agagtaattt ttatttaact cttttgggct
    10981 ttctccacaa gtaatccaat aatctataaa atttgtagaa aacatcttcc attctgtcat
    11041 atttttagca aaagatactt ttccaattcc cggattattc cataaaagtc cataacctag
    11101 actagaaata taaaaaggaa ctgatatttg agaatttctt tgggctaact ccaaaacagt
    11161 attctttaaa tctaaaaatt tatgttgata ttgtcccatt ccaaaaatct tttcagaagg
    11221 ctcagattca aatctagtag ttacttcaaa ttctccacta tagcttggtc gatattctct
    11281 tgattttaac tttagagtag agttaaagtc tttggtaatc tcaattgtcc ctacatcttc
    11341 tccaccatca tcatgtttta cagctcttaa tcgaatatat tctttcaata atatttcatt
    11401 tttatcatta taaaaagtaa ttctatctct atggtctaag acagctttta tctttccatt
    11461 tttaatgatt gcacttccat cttcattttt attaattgta atatcatctt tatttaactt
    11521 aggtttttcg ttaagagcat agttttcatc aacaaaattt tggtctacaa acgacctaat
    11581 acgtaaagag ttttttcccc atgcttctat tcgcaatagt tcattgtcaa atcttttttc
    11641 aatatagtct tcagatattt ttatcacggc tacctcctaa atcaatggta taatattcta
    11701 tctttgtgta gcttaagtta aataatgtcg tttagtatcg ttatttataa attaggtttt
    11761 atatataatt catttgtaca ttaaactaac ttaccaataa tatactttat ttgactttgt
    11821 ttgtcaatcg ctttccggat tttagtaaaa ttttctaata tattgctgga aagtcgttct
    11881 taatatcttt tatttatact tttaatatct gtgatgatac ctatatacat ctagtttttt
    11941 taaaacacat acaggaataa agtttgccta ttatgtagat aatatcggac ttgaaaatat
    12001 gtatagtgac cttataaata cagataataa tatattagat attgttcaaa acaatggatt
    12061 ttataattat aaataattta tgaa
//
LOCUS       DAKBNN010000119        12003 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000119 DAKBNN010000000
VERSION     DAKBNN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.120"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(89..319)
                     /locus_tag="OUB02_002585"
     CDS             complement(89..319)
                     /locus_tag="OUB02_002585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886330.1"
                     /translation="MKKYTKDLHIMSERNSYSKTDNDATFMRMKEDHMKNGQLKPAYN
                     IQFGVDSEYIVLVSSGHQPTDTITLIPFLKSI"
     gene            complement(984..2303)
                     /locus_tag="OUB02_002586"
     CDS             complement(984..2303)
                     /locus_tag="OUB02_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8886331.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(2344..3090)
                     /locus_tag="OUB02_002587"
     CDS             complement(2344..3090)
                     /locus_tag="OUB02_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCT8886332.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERDYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(3334..5343)
                     /locus_tag="OUB02_002588"
     CDS             complement(3334..5343)
                     /locus_tag="OUB02_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886333.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEAMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(5781..7214)
                     /locus_tag="OUB02_002589"
     CDS             complement(5781..7214)
                     /locus_tag="OUB02_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT8886334.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDILQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(7230..8654)
                     /gene="ascB"
                     /locus_tag="OUB02_002590"
     CDS             complement(7230..8654)
                     /gene="ascB"
                     /locus_tag="OUB02_002590"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCT8886335.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYNRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(8741..10657)
                     /locus_tag="OUB02_002591"
     CDS             complement(8741..10657)
                     /locus_tag="OUB02_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8886336.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(10731..11591)
                     /locus_tag="OUB02_002592"
     CDS             complement(10731..11591)
                     /locus_tag="OUB02_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8886337.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
BASE COUNT         3782 a         2251 c         1203 g         4767 t
ORIGIN      
        1 ttttatattt ctctaaaaat gtatagtttt catcactttc atacccagaa tcatccacta
       61 ttttaggata tttaaacttt gtatggaact aaatagattt taaaaatggt attaatgtta
      121 tagtatcagt gggttgatgt cctgaactta ccaaaactat atattctgaa tctacaccaa
      181 attgtatatt atatgctggt ttcaattgcc catttttcat atgatcttct ttcattctca
      241 taaaagttgc gtcattatct gttttagaat aactatttcg ttcactcatt atatgaaggt
      301 ctttagtata ttttttcaat ctatcaatac aatcatttaa ttgctctata gatttgttct
      361 ttccctttcc atgaacaaat gttatatttg attcttttga tattttttaa ccttctttaa
      421 aaatttcctt aaatgatgga ttttaatttt atctttataa attattttaa taccaaaatc
      481 aatctcacat tttaatatga agtcagatat attattcatc attttagcca tatttttatt
      541 tatagctttt ttcaaacaaa agtatatcta ttagcagatg actctatttc tcttgaagaa
      601 taaatacagt taatgtttgt atatattata attttcaaaa gttgtctagg ggttactcta
      661 tttcctctaa ttcgagcata agtcttatat aatttttcta aattcatcta ctcaataatt
      721 tgacttaata accttacaga atcatctttt ggaatcatgt aatcaatact tagtggtaat
      781 ttaaattgat aattatcgcc ataagtagta taattaaact atataccatt ttgttttaac
      841 acacaattat tatacaagac tcccaaactc ttttgaactt gggattcttg tataataaag
      901 ggtgtagcac tattttctag tgatacagcc ccatttccct aaattcaaag taatatttat
      961 agaatctcta tagattacaa taattatttg acttctacat cattatattt ctttgaatat
     1021 actttagtca tatagaaata tataggaact acaattatca tcccacctaa tccccaaact
     1081 atcttcatag gttcatcaat acctgctttt atcagtaata atatacttat aataactgca
     1141 aatattggta taattgctcc tccaggtatt ctaaaattta catctttacc agcatacttc
     1201 tttctcaata ttagtacagc tagacatgta ggaatatatt gaataaatct agatacaaca
     1261 cttatttgag ataatgttgt gaatgaaccc gaccatgcta ttagtaaagt tcctatgaca
     1321 gatactataa ttgctatata aggagctcca tttttattag tttttcttat agacttaggc
     1381 attaatcctt gttcaaccaa agcagcagca gaccttggtg taaatatgga tgatgcaata
     1441 tttatacctc caacagatac tatagttcct attgtaatta tatacccgcc tataggacct
     1501 aacatcttaa tggcagcatc tgcaacagga gtagctgacc ctgctaagcc attacccaac
     1561 acccctatac ttattcccag tataagcata tatattactg atactacaaa cattaccatt
     1621 accaatgctt taggtacatt tttcttaggg ttctccatat cttttgctgc aacagctaaa
     1681 gattcaaatc ctgtaaaagc ataaaataaa gttatagata ctgctacaaa agctgatgac
     1741 gcactttgac ctttttgtat tattacaaat ggggtaaaat tagaaccctt tataaagaat
     1801 aatcctacag ctataaataa aactattggt aataattttc ctatagtcat gatattattt
     1861 aagtgcttag attgtttcat tcccataaaa tttataatcc caagccctac aactaatata
     1921 ccaattatta tatttttagt aagtggttct gctgcttgtg gccatacaga tgatagtgtt
     1981 acagcaaaga aatttgccat agtagcccaa gcaatcatac taactatcca cttcataaat
     2041 ccaacttcaa atccaacaaa atctccaaat gcctcttttg cataaagata gggccctcca
     2101 tcagtatcaa accaactacc tgcttcagca aaacataaag caatagaaac agctaaaagc
     2161 atacaaaaaa ctaatactaa tatacttgct ggacctatat ctgtataagc atcaccaggt
     2221 aacccgaata tacctgaccc tattattgca ttgattccta atagtacaat actccaaaaa
     2281 cctaacttaa tgcctttttc cattgttatt ccccccaaag ccaacttaat atatttatat
     2341 tttttattta gatgcttctt taactattgc cataaatatt tttttcatat tatcataatc
     2401 cctagtcatc atttctggat gccattgtat ccctattaca aactcacttc cttccttttc
     2461 tatagcttca attaaaccgt ctttagatgt agctgaaaca atatatcccg gtgctacttt
     2521 gttgacaact aagtgatgaa aactattaac taaaacttct ttttctccta atatttcata
     2581 taattttgtc ccctccttta tctgtactgt atgtgtaggt acattagata gatgttgctg
     2641 attgtgttta atataagcac cttcaattaa agataaatct tgatacaaac tacctccttc
     2701 agttacattt ataatttgtt ctcctctaca tatacctaaa actggttttt tcatatctaa
     2761 agcatgtttt aataatttca tatcaaaact atctctcttt ggtgatattg cccccaactt
     2821 attatgaggt tcttctttcc atatgagtgg atttacatct tgtcccccag atagtataag
     2881 cgcatctata ttagatacct gttcctttat aatctcatca tcatatacaa tcggtacaat
     2941 atatggtata gctttacaca taactactga ttgtatataa tcattattta cataatccct
     3001 ttcatagcca ggaaacattc ctccttcatc aattagtaaa ttacctgata ttcctattat
     3061 tggatatttc tttttagcca atttcttcac cccttaactt atttgtgaac aataaaaaat
     3121 gctacttctc ttaaagagca agtagcatac caagatttta tttatttgaa cattataatt
     3181 tattttagaa atactttttt tgaatacgag taatataaat aactttattt acttatattt
     3241 tattttaatc ctgtttagtt tgactttttt aaattgtttt aagtacaact aataggtaaa
     3301 ataggcattt agattttcct ttttatcaac ctattaaata ctgtttaata tattttttcc
     3361 ccttctattt aattcacttc cctttctacc agattcacat attatcaaat ccagctcttt
     3421 taacatatta agtattcccc ttacctcacc ttctgttata ttgtagccta attcatttat
     3481 tgaattaaca ataccatttc tacctatatt tttattaaga ttattggcat tttttaaaca
     3541 ctccattact aactttactt tctctataga tgttctatcc tttaatatac ttatttcttt
     3601 actaaaatct tcagttatat ttaataagtt atgaggtaaa tcatttatta aaataacatc
     3661 ctcagaactc ataatgttaa tatacatagc agtatttttt aattctctta tattacctgg
     3721 ccattcatat gcattaataa tgtcttgaac atcttgacta attgctctct tccttttcat
     3781 aaaatgcttt aacatagctt ctatatcttc ttttcgctct cttaaaggtg gtatatttat
     3841 gggcaataca tttatcctat agtataaatc tgctctaaat ctatctcctt ttatcatttc
     3901 taataaattt ttatttgtag ccgcaattac tctaacatct atatctatta cattttctcc
     3961 tccaaccctc attacttgat tctcttgtaa tactctaagt aatttgcttt gtaagtatat
     4021 aggcatgtct cctatttcat ccaaaaatat agttccattg ttagcctgtt caaataatcc
     4081 ttttttacct ccttttaaag ctcctgtaaa agcaccttct tcatatccaa aaagttcact
     4141 ttcaagtaaa ttttctggca tagctgcaca gtttatcgca ataaatggct gactatgtct
     4201 tggtgatgca ttatgaatag actgagccat gagttctttt cccgttccac tttctcccgt
     4261 tataagaatt gtataatcag attcagctat tttcatacct aatttcttag ttctttccat
     4321 cttcgttgaa actgttttta tatcatcaaa agtatatcta gcaacttgtc ccttttctct
     4381 aagcttcttt gataggtttt gctccaattt tcttatgtaa gttatttctt gaatacaaaa
     4441 ataatatcca gtaacccttc caaaactctc tatattatac ttatttacgt ttaagtactt
     4501 attattaaat tttataactt catcatgtgc cttactcaaa ctaaaaatct ttttaatatt
     4561 ctcattctct aattcatcta tatttttccc tataatatta ctttgaatat ctagtatatc
     4621 ccctaaacta ttattgcaaa taattatgtt tcccttatct gataccattg ccatacctaa
     4681 gtttgataaa tttattatgg aatttagttc atctgctttt atagtaagtt ctttgtattt
     4741 agtatttatt cctgaatata aactaattat ttcatctgta tacttaatta gcctttcagt
     4801 aatttcttta tcatccagtt tgagttttgc tattatttga ataaacgttg aactatcaat
     4861 atacctatgg cctaaatcaa taacttcttc tatatatttt ggaactaaac ttgcctctcc
     4921 tggcgttata gctatcttaa tatttctata ttcttcattg ggattgtatg gtataaaatt
     4981 cagatgattt attcctatat tgtataaatt agatattgtt tccaatacag tatgcttatt
     5041 atcgtttact actaatacat ctattccttc tggcaatgaa aataacttat atatatcctt
     5101 ttgctttata ctcctattta tagttattat ctttgagaca tcttctacat atttttttat
     5161 ttttaatact ctatcatcta tcattaccaa tattatgtca tcttttatta attccttctc
     5221 tgttatttta tttaaataat aattttttat cattacttta tctttaaata tcatttctag
     5281 atttttattt aagaaacatt ctaatttaga caacccatca gttactactg ctattgtttt
     5341 catttgatta ctccttttaa aatatactct actcttttat aatatacatc tgattttcac
     5401 tttaacagca caaaaaattg tacatttagt atactaacat attcctttat tagttttatg
     5461 tccctaaata tcatagttgc aatatctatc atatttccta aacttctata gagcaaaaat
     5521 tgtgccaaat taataatttt cttaatttta ttctaaagtt cctcaatagt atttacagac
     5581 ttagcactta tagcttataa gtctttatat tattaatcta tttttatagg ttaattgtac
     5641 aaatcatata tcacttttac ccattaaatg cattataaaa aagatggaat aacgattcgt
     5701 tactccacct tttttgtaat aaaaaattgt atatataaac actatttact actcttctaa
     5761 gtacaaacag tatctacata ctataacctt tcaccattag attctattac ttgtttatac
     5821 cagtcaaaac tctttttctt aatcctagac aagtctccac ttccatcatc atacttatcc
     5881 acgtatataa acccatatct cttggccatc tcaccagtag atgcacttac taaatctata
     5941 catccccatg gagtatatcc aaataaatca acaccatctt ctacagcttc cttcatttgc
     6001 tctatatgtg cttttaaata ctctatacga tagtcatcat ttatcactcc attagattct
     6061 tttttatcat aagctcctaa accattttca actaccatca taggtattct atatctatca
     6121 taaatttcat ttagtgcata tctaagccct tcagggtcta tttgccatcc ccattcacta
     6181 gacttaagat atggattttt cactccacct aatatgttgc cacttgtatt atcttcttta
     6241 gaatcactac ttacacatgt agacatataa taactaaatg tataaaaatc tacacaacct
     6301 tcttttaaaa tttctaaatc tcctttttcc atttttattt caatattatt ttctccaaaa
     6361 tatctattca tataagttgg atattcgcca cgcacttgta catctccaca gaaataattc
     6421 attactctac tatctctttg agttttaact gcatcatctg gattacaagt ataaggatac
     6481 attgttgcaa atatttgcat acatccaact tgataatttg aatctatttc atgagctaac
     6541 ttaacagcct tagcacttgc tacaaattga tgatgtaacc cttgaaaacg aagttgaagt
     6601 atatctactt ggtttataaa atctgtagtc ccttcattta atattccttg tgataagtag
     6661 cctcccatag gcattgtagc tgaatttatt tcattaaatg tcagccaata ttttactttt
     6721 cctttatatc ttttaaatat tgtttcacag taattcatat acaagtctat tacatctctt
     6781 gaaacccatc cattgtattt ctctgttatt gcaaatggta tttcatagtg agatattgtt
     6841 attaatggtt ctatattata ttttaaacat tcatctatca cattttcata aaatttaagt
     6901 cctgcttcat ttggttcttt ctccattcca gttggaaata ttctagtcca tgctattgaa
     6961 aatctaaata ccttaaatcc catttctgca aataaagcta tatcttcttt gtatctgtga
     7021 taaaaatcta ttgcttcatg acttggataa aatgttcctt tttctataac tctcgttatt
     7081 cttttactta ctttttgaga acctccagta cacatatctg aacaagaagg acctttccca
     7141 tctaaattcc atgctccttc acattgattt gctgctgttg ctccacccca taaaaaatct
     7201 tttttaaatg acatacctat atcctcctat tataattctt caccatttga ttctattact
     7261 tttttatacc aggcaaaact cttcttttta ctgcgtttta aagttccatt tccatcatta
     7321 tctttatcca catatataaa tccatagcgc ttcttcattt ctccagtact tgcacttact
     7381 aagtcaatac atccccatgg tgtgtacccc cataaatcaa caccatctaa ctcaacagca
     7441 tctatcatgg cttttatatg agcacgtaaa taatcaattc tataatcatc atttatattt
     7501 ccattttcat ctggattatc tactgctcct agtccatttt caactataaa taatggtttt
     7561 tgataacggt tatataaaga gttcatagtt attctaagcc ctaatgggtc tatttgccat
     7621 ccccactcac ttgcatctag atatggattt ttaagacttg caaaaacatt tccttctgtt
     7681 aaaatattta cttttgggtc tgcacttgta agtcttgatg agtaatatga gaacgaaata
     7741 aaatctactg tgttctcttt aagtatcttt ttatcatctt cttccatcac tatatttatt
     7801 ccttttctct ccaattcttt taatgcatat gttggatatt ctccacgaga ttgtacatct
     7861 ataaaaaagt aattttccct atctttttct atagatttcc atacatctag tgggttacaa
     7921 gtattaggat aagtatttcc tgctgctaac atacacccta ttttaaattc ttgatttatg
     7981 ttatgtgcta gtttagtaac taacgcactc gcaactaatt gatgatgtgc agcttggtat
     8041 tttatttgtt cttcattttc tccctcttca atatagagac ctgcacctat aaatggtaaa
     8101 tgaagtatca tgtttatttc attaaatgtt agccaatact ttaccttacc tttaaacctt
     8161 gtaaaaataa cttcacagta ttttacatat aagtctacta gcttacgatt tctccatgct
     8221 ccatattttt ttaccaaata catgggaaca tcaaaatgac agattgttac tactggttct
     8281 attccatact ttaaacactc atcaaataaa tcttcataga attttagtcc ctcttcattt
     8341 ggaacatcct caactcctgt tggaaatatc ctagtccatg ctattgacat acgatagcat
     8401 ttaaatccca tctcagcaaa taattttata tcttctttat aattgtgata catatctatt
     8461 gcttcatgag ctgggtaata gtgttcatca tcacattcaa acattttcat tttcccagcc
     8521 aaaactggaa atctatcttt tccttgcggt gtaacgtcaa cattagctag acctctacca
     8581 tcttctttat atccaccttc acattgattt gctgctgttg ctccacccca taaaaaatct
     8641 tttctaagtg ccataattct acctccaata atttatttat agtaacttta ttttaaaata
     8701 aaaatacata tatataattc ttaattaata gaactctctt ttacgctaaa acagttatca
     8761 atttctgact attatttaca tgcttttgtg tattttctac aatatccaca taatcagaat
     8821 aattagtaac aactactggt gtttctaagc aatatccttc ttttgaaata ctttctatgt
     8881 caaaagttat taatgtctga ccttttttaa tcttatcccc ttgattaaca aaagatttga
     8941 aatatttacc ttccaactga atagtattta accctatatg tatcaatagt tcacaaccat
     9001 tatcagatat aattcctatt gcatgttttg ttggaaataa agtcattaca gtcccatcaa
     9061 atggtgccac tacttttcct tctttaggtg ttattgctac accttttccc aatatacctg
     9121 ttgaaaacgc tgcatcttgt atctgctcca aagcctttac ttcacctttt attgggctat
     9181 atactacttc tcttttgttt ttttctataa cgccttgccc attatcatct ttagaaattt
     9241 ctactatagc atcatctttc caaaaaaata atgttaaaac aaatcctact acaatagcta
     9301 ctattattgc tattatagtt gcataaacac ctttcatatc tccagttgtt ggatttatat
     9361 aattaggtat accaaatatt ccaagtccac ccattacata actttttaca ttcattatcc
     9421 ccattacagc tcctgccaca ccaccaccta cacagctata tatgaatggt gtttttcttg
     9481 gtaaagttat tccatatatt gcaggctctg ttacaccaca tatccctgaa ataactgcag
     9541 gtatacacag ctcttttatc ttcttatctt ttaatttaaa gtacattgct gctactactg
     9601 ctgtttgtgc aaaagatggt acaaatgctc ccactaatat tgtgtcaaac cccatagtca
     9661 taaggtttga catagctatt ggtactaatc cccaatgtag accaaatatt acaagcactt
     9721 gccacaataa accagttaat gcaccacata aaattggtga aaagttaaat aaagctaaaa
     9781 atccatttcc aactatatta gaagcatagg tagctatagg ccctataacc ataaatccca
     9841 atggtaaaga aactaataaa actgccattg gtacaaagaa attctttact acatctggta
     9901 ttattttagc aaacactttc tccaacttag ctgctaataa tactattaaa attactggta
     9961 ctactgttga agtgtagttc attgctatta ctggtaatcc taagaaagtt atatatactg
    10021 gtgattcaaa tattgttcca gtaaatagat tgtataaagg ttgacctgta gatgctagag
    10081 tatctaattg aatacctgaa tagcataaaa ctgcacctat aagcattcca ataaatggtt
    10141 ttaatttgaa tttcttagca gctgtatatc caagaattat tggcatatac tggaacattg
    10201 catctccaat tgcatttaac ataatatatg caccatcagt ttctccatac aaacccaatg
    10261 ctgtaaatac tgcattaaat ccttttatca taccagcagc acacagaact cctaatattg
    10321 gttgaaaaac tcctgatatt atgtctataa acttattaag taatcctttt ggcgcatctg
    10381 aatctgaatt atctattgta tctcctccta atccagccaa atcgcacaca acttcataaa
    10441 catctggaac atgattacca ataactactt ggtattgacc tgcacttttc attacagtaa
    10501 ctataccatc catttttttt agcacatcat catttgctat tgactcatct tttaatttaa
    10561 aacgtagcct cgttatacag tgagataaac tgctaatatt atcctttcct cctacattcg
    10621 caatgatttc ttttgatagt tgttcatact ttctcatttt ttctcctcca ttttcttttt
    10681 gaaatatgaa ggtttggcca acttaatgtc accacccaat atatataaac ctaattatta
    10741 ggatttatat ctgacttaat acttttactt accactcttt caacgtgaat agttaagtat
    10801 aatttttcct catctgttaa atcgtagttg tattttttat gtataaattc atccaacttt
    10861 tctatgaatt tataagcatt tttatattta acttttatta catttaataa atcatcttca
    10921 ttattttcat gtaaattatt actaacaatt cgttctgcaa aatactttaa gtgagtaata
    10981 aatctatgat aataaactga atcttcatca aatactattc tgtaatggta tttaaccata
    11041 tttgaaattt cttgtattat ttgtgtaatc tcatatatag tttttatact ttgatccatt
    11101 tgtgcattaa ctatatgcag tgctataaat ccagcttcat cttctgatag tctaatttca
    11161 aatttttttt ctatataatc caatgcctct aaccctattt taaattcact cttataaaat
    11221 ctttttatat cccaaagtaa tacatttttt acacttactc cttcacgagc tctttctatt
    11281 gcagtataca tatggtctgt taaagaaata taaatactat catttaatct tttccctagt
    11341 ttttctttag catataaaat tatttcatct gataattcca tatatcttat aggaatatct
    11401 gctattagtt ctttaaattt atcagatata tttgggtctg aaagtttgaa tatcttatca
    11461 attttatcct ttgatatatg ctctcctgta cgttttttat aagctaaacc tctccccata
    11521 actactattt ccttatgatt ttcatcagtt gtaataatta cattattatt gagtattttt
    11581 tcaataatca ttgtgatgcc ccctttatta gcattaagtt aaaataaatc ttttttaatt
    11641 atttagaata tttaaaatgt aatttattac ataaaaaaaa acctatttat tacacattaa
    11701 atagactccc atgagtcctg agtaataaat aggtttagct tgtatttaaa caatcactat
    11761 ccgtttgtat tttattttaa ttattattta ttatatgtta gcaaatttat tttttagtgt
    11821 caactttttc tcaatatttt aattttaaat aaatttattt ttgatattta tatttatttt
    11881 tttgatatta acattacaaa ttcatcctaa tttttataaa taaaataata aattaacttt
    11941 ctaataaatt ttttgtatta ttttgtatat aggaaattat tgaaatcata aaaaatcaac
    12001 gct
//
LOCUS       DAKBNN010000120        11957 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000120 DAKBNN010000000
VERSION     DAKBNN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.121"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            167..307
                     /locus_tag="OUB02_002593"
     CDS             167..307
                     /locus_tag="OUB02_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886338.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(971..1291)
                     /gene="rhaM"
                     /locus_tag="OUB02_002594"
     CDS             complement(971..1291)
                     /gene="rhaM"
                     /locus_tag="OUB02_002594"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCT8886339.1"
                     /translation="MNTIRKCFKMKLFKGFENEYKKRHDELWSEMIDMIHEYGGSNYS
                     IFLDEETNYLYGYIELKDAKLWDKSSETAICKKWWNYMSDIMETNEDNSPVSIELKSM
                     FYLK"
     gene            1477..1758
                     /locus_tag="OUB02_002595"
     CDS             1477..1758
                     /locus_tag="OUB02_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886340.1"
                     /translation="MKDETLEDILKQKTQAERYILEYIVTKNVKKLDNKKIVSILHTI
                     LCWEEINGTIVLTDKNILSLDEDIIISKHRRFIDIPKHKHNFIEFHLMS"
     gene            1882..2853
                     /locus_tag="OUB02_002596"
     CDS             1882..2853
                     /locus_tag="OUB02_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8886341.1"
                     /translation="MRQYIHDKLREITEEEKNILEGNYIIDKSIYTDNSQFIIDSNKL
                     LNIDELIHIRKHTRFAQFPKHKHNYIEFNYVYRGKLVQTIDEYKINLKQGELIFLNQH
                     VIHEIEASDEEDIIINFIIKPEFFDYIISLLENDNKIMNFLMTTLYTNYNLGEYIYFK
                     VSEKEEIQDIIEKIIIELYEPSVVNKVKIKLLVGLLLVELVKNSEDVENHAVDNYEKM
                     MIIEILKYIDKDYKKGSLSEIASNLNQGDYKISKLVKKHLGYTFKDLVQEKRLSKACE
                     LLVSTNLSIAEIIENVGYENLTYFYKIFKNKYGITPKEYKKNIKNSQ"
     gene            3060..4454
                     /gene="rhaB"
                     /locus_tag="OUB02_002597"
     CDS             3060..4454
                     /gene="rhaB"
                     /locus_tag="OUB02_002597"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCT8886342.1"
                     /translation="MRYYLAIDIGASSGRHIISYINNGKLEIEEVYRFKNNLIKKDGH
                     FCWDISKLFSEIKNGIKKCIKINKIPVSVAIDTWAVDFVLLDEKDNILGNCVSYRDNR
                     TDGIMEEAFKIIDKKELYLKTGIQFQKFNTIYQLLAIKNQQPDLLEKAKSFLMIPDYF
                     NFLLTGKKVTEYTNATTTQLVNTTTNNWDEEIINKLGFDKMLFAKIKTPKTILGSLKK
                     ELVEELGCDLEVILPVTHDTGSAFMSTPVKNRSILLSSGTWSLLGVELERPKCKLEAL
                     DYNFTNEGGYEYRYRFLKNIMGLWMIQEARKCYDSKHSFTELVEHARLSEGFKSIVDV
                     NDDRFLNPENMVQEIIKYCIETNQTEPQTVGEVAYCIFNSLAKSYSKSINEIEKIIEE
                     KLETISVFGGGCQNELLNELIAKESGKKVLAGPVEATALGNIVCQLIAKNELNDLEQA
                     RDIIRNSFDIIQYN"
     gene            4475..5731
                     /gene="rhaA"
                     /locus_tag="OUB02_002598"
     CDS             4475..5731
                     /gene="rhaA"
                     /locus_tag="OUB02_002598"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCT8886343.1"
                     /translation="MELKQRYEVAKKEYEKWGINVEEVLRVLSKVKISIHCWQGDDVT
                     GFEGNQQRLSGGIASTGNYPGKARNPQELRRDLDKALSLIPGKHKVNLHAIYAETGGS
                     FVDRDELKPEYFENWVKWAKERGLGLDFNPTIFSHPKSSDGLTLSHPDKKIRDFWIKH
                     CIASRKIGEYFGKELGQTCLTNIWIPDGYKDIPSDRLGPRRRLKESLEEILKVEINRE
                     YNLDCVESKVFGLGVESYTVGSNEFYLNFASKNNIISLLDTGHYHPTELVSDKLSSML
                     LFSDKVALHVSRPVRWDSDHVVILDDELKEIAKEIVRNDALDKVIIGLDFFDASINRI
                     AAWVIGTRNMIKALLSAMLMPHEELRKLQDEGDFTKRLAIMEEFKTYPMGDIWNYYCK
                     INNVAVKEDWIEVVKEYELTELLNRK"
     gene            5742..6557
                     /gene="rhaD"
                     /locus_tag="OUB02_002599"
     CDS             5742..6557
                     /gene="rhaD"
                     /locus_tag="OUB02_002599"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCT8886344.1"
                     /translation="MSIMEMNFIKDFMKITYDAWLKGWHERNGGNITYRLTPEEISGI
                     KEFITEEIEYIPIGVTVENLANEYFLVTGSGKFMRNISINTVQNVGIVKIDEKGENYA
                     IVWGLDKAKPTSEFPSHLMSHSIKKDKTNGLHRVIMHSHTTNLIALTFILPLEGKVFT
                     RELWEMATECPAVFPEGIGIVPWMVPGGIEIAKATQNLMEKHNIVIWAHHGTFCSGET
                     LDLTFGLMDTVEKSAEILVKVISMGGKKQTITGDNFRDLSKNFKVSISEDYLY"
     gene            6834..8300
                     /locus_tag="OUB02_002600"
     CDS             6834..8300
                     /locus_tag="OUB02_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCT8886345.1"
                     /translation="MKRKVTIQKQAISIVLFMAVLFCGLLLPMINNKPEYSLPIESIM
                     FISASIISLFIVVTTNKTWEEVFESGLDGVKGALPGLIILLVIGPLIAGFIMSGLMPM
                     LVYYGVKLINPRFVYATALILSALFSSFTGTSWGSAATIGVVMMGVGNAIGANNAIIA
                     GAIVGGAYFGDKMSPLSDTTNIAAIASKVNLYDHVASMLWTTGPATIIALVIYIACGF
                     IFPATNTNLNSPEITRLLADLSGMFNFNIILFIPLLIVLVGSAMKKPAIPVMTIAAFL
                     SLAFAVIFQGFSLADVLIGLNSGFSLDMVTWHEYAKPIEGQAYILGFFQKGGFWELGK
                     LLPISTSILFVVGVLGSIDAMPATVNVIFGWVKSRTAIICSSLLTGIAMIAMTANGIA
                     CSFVTAGIFGDKYDENNIDRRVLSRTTEDTGTLLEVLFPWTPAAIFFTQTLGVNVGEY
                     ALWSIVNWGTPIIAVILAVTGIGTYKNSKDKKQSNKVS"
     gene            8400..9590
                     /locus_tag="OUB02_002601"
     CDS             8400..9590
                     /locus_tag="OUB02_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCT8886346.1"
                     /translation="MDKNKYKIETLMNHVGENLDGDYGAVNPPIYQSSIFARSTYEDI
                     EAYFEGSKDGYLYSRTKNPTNDILEAKLSALANAEASITFGSGMGAISSAILHFVKNG
                     DHIIASNNLYNPTMNFITKELKEKGNIENTIVEGDRIEDFENAIKQNTTLIYLECPST
                     SVFKMQDIKAIVALAKTHNIKTVIDNTWATPLYQRCIEMGIDLEVHSLSKYLNGHSDV
                     VGGCIIGSKKDIEEIRGSELALYGAKLAPFEAFLVIRGLRTLHARLSVHTKNAKEVVE
                     FLDNHSAVTKVYHPLSKYFNQKELAKKQLLNYTALLSFEIDAKDVNDVKKFVNKLDLF
                     KIGVSWGGHESLVFAPNIAILNEMTLEQAKALNVSPYTIRISLGLENSEDLINDLNDA
                     LSIL"
     gene            9783..11741
                     /locus_tag="OUB02_002602"
     CDS             9783..11741
                     /locus_tag="OUB02_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HCT8886347.1"
                     /translation="MKRAYYSSSVNEFIVKDIFTIFGEITCNDQFAADDLQKNTWKKE
                     IGILKRELSTFKDGHLLFEYTIPRIGSRIDNVLIYKGIVFLLEFKVDEDTYPRHAIEQ
                     VTDYALDLNCFHKESHDKLLVPILICTKAPQKDQRIRMMKENILETYCCNEFNIGKYI
                     KKICTTYNRSSFNSDTWINSLYMPTPTIIEAAQALYMGHNVEDISRNDASAKNLNQTT
                     KAINKIIDYSKTHNRKSICFITGVPGAGKTLAGLNIAIERQKVDENEHAVFLSGNGPL
                     VDVLQEALARDDVTRNGIRKSEAIRKAKEFIQIIHHFRDEAISVDTPPIERVTIFDEA
                     QRAWDEPNLTNFMKRKKGVLDFNMSEPEFLISILNRHIGWATIVCLIGGGQEINKGES
                     DGISGWFESLRNNYSDWDVYISNKIIDEEYSKGNSFDELTEGVNYRIIDDLHLSVSLR
                     SFRSENVSTFVKAVLDVDKVAAKELYNQFKKDYPICVTRDLELAKKWVRDKSKGSQRY
                     GMTASSGAKRLRKYGVWVQNKIDATNWFLNGKDDVRASYFLEETATEFDIQGLELDWT
                     IVCWDGNLRFKNNHFEYYNFNGTKWQNINKEDNILYLKNAYRVLLTRARQGFIIFVPI
                     GDESDITMQPNFYNGVYEYLKEIGIEEL"
BASE COUNT         4835 a         1283 c         2052 g         3787 t
ORIGIN      
        1 aaaattatta attataaaaa ataataataa agacgataag tattaaaatt ttatgtttcc
       61 aatatttatg atataattaa tatggcaagg aataaatatt tgaaaaagct atgagtggtg
      121 tttccatgga agtttcttca ctttcttatg agaggaggtg aaaactatgg aaaatctaat
      181 gatgagtatt ataactggtg ttatagccag ttacatttac gataaattaa agagccactc
      241 tgagcgacca actaagagtg gctgggaact taatataaag ttccagaaaa gtaaatcttc
      301 aaaataattt gttgaaggaa acatcactca aagttaagta aaatatgttt ccttgctttt
      361 attatatcac aaactaaaaa tattaacaca aaattttgtg aatatatact tattattgtt
      421 ctaaaaaaat atgtaaataa ttgatttgat ttcatagaat tattgttgta atgtttagtt
      481 aaatatacac aatctcaatt aaagaagata agtactataa attcatatca aaaaaatata
      541 taaactttta agttataatt ttataaaaag tcttttagta tcattatttc aagaggaatt
      601 aacatatgaa aaaataactt cattagaaaa gtggataaaa aactagtaag aagatgacta
      661 aagattacat atactattac ttaaattaaa aatttaatat aaacaacaca agatatatca
      721 attattctaa agttaagtag tcttactgaa tcatctttgg gaataattgt ttcaatgtct
      781 agtagaaata ccacttgttt ataacatctt tagctactat aatcgaaata tgtttttagt
      841 ttttcacaga acaattatca catagactag aggtttttag gaatcactta tcttttttat
      901 acaaaaagga gataattcaa atttttattt tgatatatcc ccattttgtg atttataata
      961 atttttgtcc ttattttaaa taaaacatag atttaagttc tatagataca gggctattat
     1021 cctcattagt ttccattata tcagacatat aattccacca ttttttgcag attgctgtct
     1081 ctgaactttt atcccatagt ttggcatctt taagttctat atatccatat aaataattag
     1141 tttcttcatc tagaaaaata gaatagttac taccaccata ttcatgaatc atatctatca
     1201 tctcagacca taattcatca tgacgtttct tgtattcatt ttcaaatcct ttaaaaagct
     1261 tcatcttgaa acatttgcgt attgtattca taaaaaaata cctcctaaat tttaagtatt
     1321 tacctataaa tgtattttaa aacaaaaaaa ggtattttta aatgtcataa tttaacatta
     1381 ttactgtcaa aaactaatat gatacaatta gataaagaat atttgtaata taatttaaaa
     1441 ctgtaataaa gtatagctaa atctgggggg atataaatga aagatgaaac gctagaggat
     1501 atacttaaac aaaaaacaca agcagaaagg tacatcttag aatacatagt tactaagaat
     1561 gtaaaaaaac tagataataa aaaaatagtg tctatattgc atactatttt atgttgggaa
     1621 gaaataaatg gaactatagt actaacagat aaaaatatac tttctttaga tgaagacatt
     1681 ataatatcta aacatcgtag atttatagat atacccaaac ataaacataa ctttatagag
     1741 tttcatctaa tgagttaata tataattttt tatcaggaac tatacacaaa ggaaaaagat
     1801 gtgataatta ttatatattt aatgctaaaa ataattttaa attgaaacaa ctagtagttg
     1861 aactaactaa ggaggtagct catgagacaa tatattcatg ataagttgag ggaaataaca
     1921 gaagaagaaa aaaatatatt agaaggaaat tatataatag ataaaagtat ttatactgat
     1981 aattctcaat ttataataga tagcaacaaa ttattgaata tagatgagtt gatacatata
     2041 agaaaacata caagatttgc acaatttcca aaacataagc ataattatat agaatttaat
     2101 tatgtttata gaggaaagtt agtacaaact atagatgaat ataaaattaa tttaaaacaa
     2161 ggagaattaa tatttttaaa tcagcatgtt attcatgaaa tagaagctag tgacgaagaa
     2221 gatataataa ttaattttat aataaaacca gagttttttg attatattat atctttgttg
     2281 gaaaatgata ataaaataat gaacttttta atgactacat tatatactaa ttataatctt
     2341 ggagaatata tatattttaa agtatctgag aaagaagaga ttcaagatat aatagaaaaa
     2401 attataatag aattgtatga accaagtgtg gttaataagg taaagattaa attattagta
     2461 ggattattat tagtagaatt agttaaaaat tctgaagatg tagaaaatca tgcagtagat
     2521 aattatgaaa agatgatgat tatagaaata ctaaaatata ttgataagga ctacaagaaa
     2581 ggtagtttat ctgaaattgc aagtaatttg aatcaaggtg attataaaat aagtaaacta
     2641 gtaaaaaaac atcttggata tacttttaaa gatttagtgc aagaaaaaag gttaagtaaa
     2701 gcatgtgaat tattagtatc tactaattta tcaatagctg aaattataga aaatgtaggt
     2761 tatgaaaatt taacttattt ttataaaata tttaaaaata agtatggtat tacaccaaaa
     2821 gaatacaaaa aaaatataaa aaatagtcaa tagtaaaatt agaatctata aaatagattc
     2881 ttttttatta ttaaataaac cattttaata gctctgaaga tattatattt tgaaaattat
     2941 tattgagaat aaagttatat tgcaaattag gttaataaat atgacaaatt tagatattaa
     3001 atgaatatac taacatcttg tataattcaa ttaataaata aatctaagga ggaaaagtct
     3061 tgagatacta tttagcaatt gatatagggg catctagtgg aagacatatt atctcttata
     3121 taaataatgg aaagttggaa atagaagaag tatatagatt taagaataat ttgattaaaa
     3181 aagatggtca tttttgttgg gatatatcaa agctattttc tgaaataaaa aatgggataa
     3241 aaaaatgtat aaaaataaat aaaattccag taagtgtggc tatagacaca tgggctgtag
     3301 attttgtgtt acttgatgaa aaagacaata tattaggtaa ctgtgtatca tacagagata
     3361 atagaactga tggaattatg gaagaagcct ttaaaataat tgataaaaag gaactatatt
     3421 taaaaacggg aatacaattt caaaagttta atactatata tcaattatta gcaataaaaa
     3481 atcaacagcc agatttatta gaaaaggcaa aaagctttct aatgattcct gattatttta
     3541 actttttatt aactgggaaa aaagttacag aatatacaaa tgcaacaact acacaattag
     3601 taaatacaac aacaaataat tgggatgaag aaataataaa taaacttgga tttgacaaga
     3661 tgttatttgc taaaataaaa actcccaaaa caatactagg tagtttaaag aaagaattag
     3721 tagaagagtt aggatgtgat ttagaagtca ttttaccagt tactcatgat acaggttcag
     3781 ctttcatgtc tactcctgta aaaaatagaa gcattttatt gagttcagga acatggtctt
     3841 tattaggagt agagttagaa agaccaaagt gcaaattaga ggctttagat tataatttta
     3901 ctaatgaagg tggatatgaa tatagatata gatttttaaa aaatataatg ggtttatgga
     3961 tgatacaaga agctagaaag tgttatgaca gtaagcactc ttttacagaa ctagtagagc
     4021 atgcaagact atcagaagga tttaaatcaa tagtagatgt aaatgatgat agatttttaa
     4081 atcctgaaaa tatggtacaa gaaataataa aatactgtat agaaactaat caaacagaac
     4141 cacaaacagt tggagaagta gcatattgta tttttaacag tttagcaaaa agttatagta
     4201 aatctataaa tgaaatagaa aaaatcatag aagaaaaatt agaaactata agtgtatttg
     4261 gtggaggatg tcaaaatgaa ctgttaaatg agcttatagc aaaagaaagt ggtaaaaagg
     4321 tattagcagg acctgttgaa gctacagcat taggtaatat agtatgtcaa ttaattgcta
     4381 agaatgagct aaatgattta gaacaggcga gagatataat aagaaattca tttgatataa
     4441 ttcaatataa ttaatctgaa aaaggagata agtgatggaa ttaaagcaaa ggtatgaagt
     4501 agctaaaaaa gaatatgaaa aatggggaat aaatgtagag gaagtattaa gagttttatc
     4561 taaagttaaa atatctattc attgttggca aggtgatgat gtaacagggt ttgaaggaaa
     4621 tcaacaaaga ctatcaggag gaattgcatc tacaggaaat tatcctggaa aggctagaaa
     4681 tcctcaggag ttaagacgag atttggataa ggctttaagt ttaataccag ggaaacataa
     4741 agttaactta catgctatat atgcagaaac tggtggaagc tttgtggata gagatgagct
     4801 aaaaccagag tattttgaaa attgggtaaa atgggctaaa gaaagaggat tgggattaga
     4861 ttttaaccca acaatattct ctcatccaaa atcaagtgat gggttaacat tgtcacaccc
     4921 agataaaaaa atacgtgatt tttggataaa gcattgcata gcttctagaa aaataggcga
     4981 atactttgga aaggagcttg gtcagacttg tttaacaaac atatggatac cagatggata
     5041 caaagatata ccaagtgata gattaggtcc tagaagacgc ttaaaggaat cactagaaga
     5101 aatattaaag gtagagataa acagagaata taatttagat tgtgtcgaat caaaagtttt
     5161 tggattaggt gtagaatctt atacagttgg gtcaaatgag ttttacttaa attttgctag
     5221 taaaaacaac ataatttcat tattagatac agggcactat catcctacag aacttgtttc
     5281 agataaatta tcatcaatgc tattattttc agataaagtt gcacttcatg taagtcgtcc
     5341 agtgcgttgg gatagtgacc atgtagttat attagatgat gaactaaaag aaatagccaa
     5401 ggaaatagta agaaatgatg ctttggataa agtaataata ggactagact tctttgatgc
     5461 aagtataaat cgtatagcag catgggtaat tggaactaga aatatgataa aagcattgtt
     5521 atcagctatg ttaatgcctc atgaagaatt aagaaaatta caagacgaag gcgattttac
     5581 aaaaagattg gctataatgg aagagttcaa aacatatcca atgggagaca tatggaacta
     5641 ttattgtaaa attaataatg ttgctgttaa agaagattgg atagaagtag taaaagagta
     5701 cgaattaaca gaattattaa atagaaagta ggtaagggta tatgtctata atggaaatga
     5761 actttatcaa agattttatg aaaataactt atgatgcttg gttaaaggga tggcatgagc
     5821 gtaatggggg gaatataacg tatagattaa cacctgaaga aatatctgga ataaaagaat
     5881 ttataacaga agaaatagaa tatattccaa ttggtgtaac agtcgaaaat ttagctaatg
     5941 aatatttctt agttacagga agtggcaaat tcatgagaaa tatttctata aatactgttc
     6001 aaaatgtagg aattgttaaa atagatgaaa aaggtgaaaa ttatgcaata gtttggggat
     6061 tagataaggc taaaccaaca agtgaatttc catctcactt aatgagtcat tctataaaaa
     6121 aagacaaaac aaatggatta catagagtaa taatgcattc acatacaact aatctcatag
     6181 cattaacttt tatactgcct cttgagggta aagtatttac aagagaatta tgggagatgg
     6241 ctacagaatg tccagctgta tttccagaag gaattggaat cgtaccttgg atggtgccag
     6301 gtgggataga gatagcaaaa gctacacaaa atctaatgga aaaacataac atagtaatct
     6361 gggctcatca tggaacgttc tgctcaggag aaactttgga cttaacattt ggtcttatgg
     6421 atacagtaga aaaatcggct gagatattag taaaagtaat atctatggga gggaaaaaac
     6481 aaacaataac aggagataac tttagagact tatcaaaaaa ttttaaggtt tcaatatcag
     6541 aggattattt gtactagata agataaaaga taaacaaagc tatgataact tattatgtac
     6601 aattaataaa acactatgag agaggaggtg attaaaagat atagaaagta aattcactta
     6661 agaaaatagt ttaagaataa actggagttt ataataaaat attcaacaat atatttatat
     6721 atataaatat attgttgaat ataaatataa gatagtaaat tttattgtta gataagggaa
     6781 aacgttatta aaaattgaat taatgagtaa ataaaagtgg gaggaaataa taaatgaaga
     6841 gaaaagttac aattcaaaag caggctatat ctatagtttt gtttatggca gttttatttt
     6901 gtgggctatt acttccaatg attaacaata agccggaata tagtttgcca atagagtcaa
     6961 taatgtttat ttcagcatca ataatatcgc tatttatagt ggttacaaca aataaaacgt
     7021 gggaagaagt atttgaaagt ggattagatg gtgtaaaagg agctttacca ggattaataa
     7081 tattattagt aataggtcca cttattgcag gatttattat gagtggatta atgcctatgt
     7141 tagtatatta tggagttaaa ttgataaatc caagatttgt atatgctaca gcattaatat
     7201 tgtcagcatt gttttcttca tttacaggaa cttcctgggg atcagcagca actataggag
     7261 ttgtaatgat gggggtaggt aatgcaatag gagcaaacaa tgctattata gcaggtgcta
     7321 tagtaggtgg agcttacttt ggtgataaaa tgtcacccct atcagataca acaaatatag
     7381 ctgcaatagc gtcaaaagtt aatttatatg accatgttgc atctatgtta tggacaacag
     7441 gtccagcaac tataatagct ttagttatat atattgcttg tgggtttata ttcccagcaa
     7501 caaatacaaa tttaaatagt ccagaaataa ctagattatt ggcagattta tcaggaatgt
     7561 tcaatttcaa tataatttta tttatacctc ttttaatagt attagtaggt tcagcaatga
     7621 aaaaacctgc aataccagta atgacaatag cagcattttt atcacttgca tttgcagtaa
     7681 tctttcaagg tttttctttg gcagatgttc ttataggact taacagtgga ttctcactag
     7741 atatggtaac ttggcatgaa tatgcaaaac ctatagaagg acaagcttat atattaggtt
     7801 ttttccaaaa aggtggcttc tgggaattag gtaaattatt gccaatatca acatctatat
     7861 tatttgttgt tggagtactt ggaagtattg atgctatgcc agcaacggta aatgtgatat
     7921 ttgggtgggt taagtctaga acggctataa tatgttcttc attattaaca ggaatagcaa
     7981 tgatagcaat gactgcaaat ggtatagcat gtagttttgt aacagcaggt atatttggag
     8041 ataaatacga tgagaataat atagatcgta gggtactatc aagaactaca gaagatacag
     8101 gtacattgtt agaagtttta tttccttgga caccagcagc aatatttttc actcagacac
     8161 taggagttaa tgttggagaa tacgcacttt ggtctatagt gaactgggga actccaatta
     8221 tagcagtaat cttagcagtt acagggatag gtacatataa aaattcaaag gataaaaaac
     8281 aatctaataa agtatcataa tgaatatttt aactttatta cctaacatag cttataaaaa
     8341 ataagctatg ttaaatgtat ttttcatata taaaaatatt tcataaggag atttagaaca
     8401 tggataaaaa taaatataag atagagacat taatgaatca tgtaggagaa aatttggatg
     8461 gcgattatgg agctgtaaac ccacctatat atcaatcatc aatattcgca agaagtacat
     8521 atgaagatat agaagcatat tttgaaggtt caaaagatgg atatctttat tcaagaacta
     8581 aaaatcctac taatgatatt ttagaagcaa agttatctgc attagctaat gctgaagcaa
     8641 gtataacctt tggttcagga atgggtgcta tatcaagtgc tatacttcat tttgtaaaaa
     8701 atggtgacca tataatagct tcaaataatt tatacaatcc tacaatgaac tttataacaa
     8761 aggaattgaa ggagaaagga aatatagaaa atacaatagt tgaaggtgat agaatagaag
     8821 attttgaaaa tgctataaaa caaaatacaa ctttaatata cctagagtgt ccatcaacat
     8881 cagtatttaa aatgcaagat ataaaggcaa ttgtagcact agcaaaaaca cacaatataa
     8941 aaacagtaat agataatact tgggctacac cattatatca aagatgtatt gagatgggaa
     9001 ttgatttaga agttcattct ttatctaaat atttaaatgg acattcagat gtggtaggtg
     9061 gatgtattat tggaagtaaa aaagatatag aagaaataag agggagtgaa ttagctttat
     9121 atggtgcaaa attagcacca tttgaagcat ttttagttat aagaggtttg agaactttac
     9181 acgctagatt atcagtacat accaaaaatg ctaaggaagt agttgaattt ttagataatc
     9241 attcagcagt tactaaggta tatcatccat tatctaaata ttttaatcaa aaagaactag
     9301 caaagaaaca gttgcttaat tatactgcgt tattaagttt tgaaatagac gcaaaggatg
     9361 taaatgatgt taaaaaattt gtaaataaat tagatttatt taaaatagga gtgagttggg
     9421 gaggccatga aagtttagta tttgctccaa atatagctat attaaatgaa atgactttag
     9481 aacaagccaa ggcattaaat gtgtcaccat acacaattag aataagttta ggtcttgaaa
     9541 atagtgaaga tttaatcaat gatttaaatg atgcgctatc cattttataa tataataagc
     9601 atatatggaa aaaacatatt aattaaatag tttataatgt acttagcaaa gaaaaattat
     9661 tatcaaagta ccatacataa acatatctat gtagtgtatt tataaagtat aggagtcaag
     9721 ttttagattg tagtatagat gtaggctata aaatgaatat tagatataaa gggggaagat
     9781 gtatgaaaag agcatattat tcaagtagtg taaatgaatt tatagtcaaa gatattttta
     9841 ctatatttgg tgagataacg tgtaatgatc aatttgcggc tgatgactta caaaagaata
     9901 catggaaaaa ggaaattgga atattaaaaa gagagctctc aactttcaaa gatggacact
     9961 tattatttga gtatacaata ccacgaatag gcagtagaat tgacaatgtg ttaatttaca
    10021 agggcattgt ttttctcctt gaatttaaag ttgatgaaga cacttatcct aggcatgcaa
    10081 ttgaacaagt tacagactat gcccttgacc ttaattgttt tcacaaagaa agtcatgata
    10141 aacttttagt accaatactt atttgtacaa aagcccctca aaaagaccaa agaataagaa
    10201 tgatgaagga aaatatttta gaaacttatt gttgtaatga gtttaatata ggaaaataca
    10261 taaagaagat ttgtactaca tataatagaa gttcatttaa ttcagacact tggataaact
    10321 cgttatacat gccaactcca acaattattg aagctgcaca agctttatat atgggccata
    10381 atgttgaaga tatttcaaga aatgatgcaa gtgctaaaaa tttaaatcaa acaactaaag
    10441 ctataaataa aattatagac tatagtaaga ctcataacag aaaatcaata tgttttatca
    10501 caggagttcc aggagcaggt aaaacacttg ctggccttaa tattgccatt gagcgacaaa
    10561 aagtagatga aaatgaacat gcagtcttcc tttctggaaa tggtccatta gttgatgttt
    10621 tacaagaagc actagctcgt gatgatgtaa ccagaaatgg aatacgaaaa tctgaagcga
    10681 ttcgaaaagc gaaagaattt attcagataa ttcatcattt tagagatgaa gctatatctg
    10741 ttgatacacc acctattgaa agagtcacaa tttttgatga agcacaacgt gcgtgggatg
    10801 aacctaattt gactaacttt atgaaaagaa aaaaaggcgt attagacttt aatatgtcag
    10861 agcctgaatt tcttattagt attttaaatc gtcatatagg atgggcgact attgtttgtt
    10921 tgattggtgg aggtcaagag ataaataaag gtgagtcaga tggaatatca ggttggtttg
    10981 aatcactaag aaataattat tctgattggg atgtttacat atcaaataaa attattgatg
    11041 aagaatattc taaaggtaat tcttttgatg agttaacaga aggtgttaat tatagaataa
    11101 ttgatgattt gcacttatct gtatcgttac gttcttttag aagtgaaaat gtatcaacct
    11161 ttgttaaagc tgttttggat gtagataaag ttgctgcaaa ggaattatat aatcaattta
    11221 aaaaagatta ccctatatgt gttactaggg atttggaatt agctaaaaag tgggtgagag
    11281 ataaatcaaa aggttcacag cgttatggaa tgactgcaag ttctggagct aaacggcttc
    11341 gaaagtatgg agtgtgggta caaaataaaa ttgatgctac aaactggttt cttaatggta
    11401 aggatgatgt acgagcttca tattttttag aagaaactgc aacagagttt gatatccaag
    11461 gtttagagct agactggaca attgtctgtt gggatgggaa cttaagattt aagaataacc
    11521 attttgaata ctataacttc aatggaacta aatggcagaa catcaataaa gaagataata
    11581 tactatactt aaaaaatgca tatagagttc ttcttacaag ggcaaggcaa ggctttatta
    11641 tatttgtccc aataggtgat gagagtgata ttacaatgca gccaaatttt tataatggtg
    11701 tgtatgaata cttaaaagaa attggaatag aggaacttta ggtttaaagt aaggatgcta
    11761 ttaagtggat tatttgtaaa aaattatctt cctttataat atatgagaaa gtaaaacttt
    11821 ttaactaaag aatacattca aaagataata agaaaaagga gagtaacaat ttgagtttat
    11881 aaataatctt aataattttc atccaatcta aaatttaaat gtggttttta ctataaacag
    11941 gacattggat tatattg
//
LOCUS       DAKBNN010000121        11939 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000121 DAKBNN010000000
VERSION     DAKBNN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.122"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            470..1183
                     /locus_tag="OUB02_002603"
     CDS             470..1183
                     /locus_tag="OUB02_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886348.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAKNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1455..2993)
                     /locus_tag="OUB02_002604"
     CDS             complement(1455..2993)
                     /locus_tag="OUB02_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT8886349.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENGQYLIKASDYQEQLIANNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            3875..4330
                     /locus_tag="OUB02_002605"
     CDS             3875..4330
                     /locus_tag="OUB02_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HCT8886350.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            4379..4963
                     /locus_tag="OUB02_002606"
     CDS             4379..4963
                     /locus_tag="OUB02_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HCT8886351.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(4968..5291)
                     /locus_tag="OUB02_002607"
     CDS             complement(4968..5291)
                     /locus_tag="OUB02_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886352.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            5443..5961
                     /locus_tag="OUB02_002608"
     CDS             5443..5961
                     /locus_tag="OUB02_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8886353.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(6137..6805)
                     /locus_tag="OUB02_002609"
     CDS             complement(6137..6805)
                     /locus_tag="OUB02_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886354.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            6920..8335
                     /locus_tag="OUB02_002610"
     CDS             6920..8335
                     /locus_tag="OUB02_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT8886355.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKEFSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            8558..9016
                     /locus_tag="OUB02_002611"
     CDS             8558..9016
                     /locus_tag="OUB02_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886356.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            9013..9597
                     /gene="clpP"
                     /locus_tag="OUB02_002612"
     CDS             9013..9597
                     /gene="clpP"
                     /locus_tag="OUB02_002612"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCT8886357.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            9909..10985
                     /locus_tag="OUB02_002613"
     CDS             9909..10985
                     /locus_tag="OUB02_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8886358.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            11052..>11939
                     /gene="bclA3"
                     /locus_tag="OUB02_002614"
     CDS             11052..>11939
                     /gene="bclA3"
                     /locus_tag="OUB02_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HCT8886359.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGA"
BASE COUNT         4462 a         1490 c         1965 g         4022 t
ORIGIN      
        1 gaatagatat aaatatatgt agatattaaa taaatagctt agatagatat tttatgtttt
       61 aataagtaat tcaattagtg gtagatatat actattgaaa attaaagtta taattgatat
      121 aaagaattaa taaaaataac atataaaagt ttaaaaggga gttgaactta aagtttgatt
      181 agaaaaagtc aattttaaga caactccttt tattttacta ttgtattaat ctgtatctgt
      241 aagtgtatta gtctaatatt ataaactttg gatgaaatta attttctata ggttttaatg
      301 tatcatacac tttattttaa agaaacttat cttaattttc atagtgattg aatttgtcta
      361 ttagagatgt tactattagt aatgtaaaag caagagtagt gaatataaaa aacaagatag
      421 tgatactgca agattagaat ataataaatt tttatggaga gatttcaata tggatacatg
      481 ttttaataaa aaaatattac ttgttgatga tgaaaaggat atagtagatt taattgaaga
      541 agtactcata aatgatggtt ttaaaaacat tattaaagct tataatggtc tagatgctat
      601 ttcgctgtgt aagatagcat gtccagatgt tgtcattctt gatataatgt taccagatat
      661 agatggtata gaagtgtgta aaaaaattcg agagttttca tactgttcta ttcttttttt
      721 atcatcaaaa aatgatgata ttgacaaaat actaggtctt agtagtgggg gagatgacta
      781 tataacaaag ccattcagtc caagagaaat tgcttttcga gttaaagcgc aattacgtag
      841 acaacagtat caaagcattg taccatcaga tagtgaagtt attaaaatag gagatattac
      901 tattgatata gaaggaaacc gtgtatataa agatagaaat gagattgagt taactggaag
      961 ggagtatcat cttttaagct acatggctaa aaatgtaaat aaaattatag gaaaagaaag
     1021 actctatgaa caagtatggg gagtatatag tagtatttgt gacaatacaa ttatggtgca
     1081 tatacgacat ataagagaaa aaattgaaga taacccctca aatcctaaaa tacttataac
     1141 agttaaagga ctaggatata aacttgtgaa tagaatcgat tagaatagat tttaatatta
     1201 atgaagatat ctcaagttga atttttagtt aatgaggcat tcttttatgg gtgaaatcaa
     1261 atgtattttc gttatctcaa caatgaagaa gaggtctatc tctattttga gatagcctca
     1321 ttaagtttat atcctggtaa tgtactttat gttctaataa aattttttta gtttatttgc
     1381 agtgaaacat taactagaac attaaaattt gcattgaaat accaattaaa taatattgta
     1441 tattgtgtat aaacttatac taattgtttt tctatattaa atactttatc aatccaacct
     1501 ttataaaatt tttcttcatg ggaaactagg attatacttc ctctaaactg tttcaaagca
     1561 ttttgtaaag catcctttgt ctctgcatca aagtgattgg ttggttcatc cagaataaga
     1621 aaattacaag gagataacat catacaacat aatttaacct tagattgttc accaccactt
     1681 aaagtagata cactccttga aacatgctct tccttcacac cacatctggc tagatgacgt
     1741 ctaatctctt ttgtatttaa ttttggatat ttatctgcta ctatttgaag tggggtttta
     1801 tcaggatttt cccattttaa atcttgctca taataaccaa ttttaacttg ttctgaaaac
     1861 tggaaatctc cagatatacg aggaatatct tttactaatg tctttaacaa ggtagattta
     1921 ccgattccat taaaaccagt tataactatt ttttgacctc catcaacaga aaaatttaat
     1981 tttggaagta aagaatagta gtatccaact tcaagatttg ttatagtaag agcattttga
     2041 gccgatattt caatttctga aaattgaata tttggttttc ctgtaaaact aggaggagct
     2101 attctttcta ttttatccaa ttgtttttgt cttcctcttg caatttttga atttactcca
     2161 gctttatttt tacgtataaa tgtttcttct ttttctattt tcttttgttg tgcttgatac
     2221 cttctaatat aatcttctct taaatgtgat ttttgtttaa ggaactctga atattttcca
     2281 tgatatttct taatcatgcc aaattctata tcaagaatac ctgtagaaat tttttctaaa
     2341 aagtcaaaat cgtgggaaac tacaataaat gccccattaa atgtatttaa ataatttgag
     2401 agccattcaa catgttcttt atctaaaaag tttgttggtt cgtctaataa tagaacatca
     2461 tggttagaaa gtagtaattt tgctaaaatt acctttgccc tttgaccacc actaagctct
     2521 ccaacaacac gattcattcc tattgaatct aatccaagac catttgctat tttatttatt
     2581 tcattatcta tagaatagaa attatttgct attagttgtt cctgataatc tgatgctttg
     2641 attagatatt gcccattttc agatatagca cttttttgat agagtagatt catttccttt
     2701 tctattttat aaagttcctc aaatgcagtg tgtaaataga gtgatattgt tgtatctcta
     2761 ttgatttctg catattggtc taaatggcct atattgatat taggttgcca cttgatttct
     2821 cctgaatctg gaacaacttc tcccaataaa attttaatta atgtactttt acctgtacca
     2881 ttttggccaa ctacacctat atgttcacct ttgaataaat caaagttagc tttctcatac
     2941 agtgatttat ccataaaaca ttgtgataaa ttagttactt ttaataaact cataattcaa
     3001 actccttttc ttttattaaa aaatttatgg aaataaaaaa agcagaggaa tggcattgta
     3061 aaatgccgac ctctgctttt ggaaaaacaa aaggtcatag acaagcattg ttgtctatga
     3121 ccagaaaatc tatattatct atcggcaaat aggtaatttt aaataagcat agccaaaaaa
     3181 ctatactaaa cctatatttg ccgatgaaga tagaaagctt taagctttca gattaacctc
     3241 tacacaacgc ttcatcgtat acatttgtgt agagttaagt tttctaacaa gtttaactaa
     3301 ttctatataa atcataatta ttcacatcct aatcttagta ttaattctag tttatctata
     3361 tttaaattgt acttttgtac tttgtggatt atcttattta tttaaaatac gttttattaa
     3421 ttatagctta agttatgtta tcataatact tagttatagt caataagaaa gttatattta
     3481 ctattattat attaatttag taaatctatc attacaaagt tatttgaaga ctcgctatca
     3541 ataaaatttt atagtctata agaaaattaa gattatgagt tttataattt tgatataggg
     3601 atactatttt cataaatatt acaacatgta gaagtatatg tctagtattg ttgaattttc
     3661 aatgatatat taaggttcta atatattata caaaataaaa acattaattc aacttaaata
     3721 attttaagta ttatatgtgt tttgattaaa gaaaactaaa attttaagga aaaataacat
     3781 atttgtatta aattttattg aaatgattaa atttatttac ttaatgtaaa ttattagcta
     3841 aaattgtatt aaataattaa ggaggtgagt agctctggat gaaatagatt taaaaataat
     3901 taattctcta aaaaagaata gtagggcttc aacctctgaa ataagcagac aaattaattt
     3961 atcagtacca gcagtctcag agcgtataag gaaaattgag cagtcaaatt taattcaaaa
     4021 gtacactatt caaattaatc gggaaaaaac aaaatataag cttttggctt ttatatttat
     4081 caatactgat ttatctaaaa gtatgaaaga atttaagaaa actatattgg aatatgatac
     4141 tgtattagaa tgtcatcata tttcaggaga gtatgattac ttaataaaag tattagcaga
     4201 agatacaagt aatcttgaag agtttatatc agtgactttg aaggaaattg gggggattca
     4261 aaaagctaat acaaccattg ttttatctac aattaaagaa gaatttaata caataaaaga
     4321 tattttttaa ataatttact agtaaagtgt taattatatt ttaggaggaa aatagaatat
     4381 gaaagtttta gtttttttag caaaaggttt tgaaactatg gaatttagtg tatttgtaga
     4441 tgttatggga tgggctcgta acgattatgg gcatgatatt gatgtagtga cttgtggatt
     4501 taaaaaacaa gttatgagca catttaatat acaagtatta gttgataaga cgatagaaga
     4561 agtatgtgtt gatgattatg atgctctagc tattcctgga ggatttgaag aatttggatt
     4621 ttatgatgaa gcatatgatt catctttttt aaatctaata agggagttta attcaaaaga
     4681 aaaaataatc gcttctattt gtgtggcagc attgccagtt ggaaaaagtg gtgttttaaa
     4741 aaatcgtaaa gctactacat atcatttgaa aaatggtaaa agacaaagac agttaagtga
     4801 gtttgatgta aatgtagtta atgaaccaat tgttgtagat aaaaatatta taacctcata
     4861 ttgtccagaa acagctccac atgtagcttt taagctttta gaaatgttga catctaaaga
     4921 acaaatggat gaagttaaat tagcaatggg atttaaactg taatggttta tagattttct
     4981 aagtaatcag aaccccagtc acacatggaa tctaaaattg gaataatact ctttccaaaa
     5041 tcagatagcg agtattctac ttttggagga acaacaggat aaaccactct tataattaaa
     5101 ccatctgatt ctaattcacg taattgttga gttaacatct taggcgttgc tttaggaatt
     5161 aattttttta attcattgaa cctcaatata gtgtctttaa gatgccaaag tataagtgtc
     5221 ttatatttac caccaatcaa tgcaagtgtt gcttctattg ggcaactgta attatctaaa
     5281 cattttttca tttttgggct cctttattta ttttaaagta tctttttagg tactatatta
     5341 caaaaaagtg cattattgta atatagtacc atagtgataa aattatagca taggttaaat
     5401 aaatcatcaa cttagcctaa taaaacaata gggggtaaat aaatgaattt cgttgaactt
     5461 gcaaaaaaaa gatattcttg ccgtaattat caagatagaa aggttgaaaa agaaaaactt
     5521 gagaaggttt tagatgttgc aagaatagct ccaactggtg gtaatcgtca acctcaaaga
     5581 ttgattgtta ttcaggaaaa agaaggtata aataaacttt ctaaggctgc caatatatat
     5641 gatgcgccat tagcaattct tgtatgtgga gataaagaca aagtttggac aagacctttt
     5701 gatgggaaac aacttacaga tattgacaca agtatagtta cagaccatat gatgttacag
     5761 gcaactgaac ttggattggc aagtgtttgg gtgtgctatt ttaatccaga tataattaga
     5821 gaagagttta gtttgccaga taatctagaa ccaataaata tattattgat gggatatgaa
     5881 tctaagatac cagagagccc agagagacat gaaaaaactc gtgttccttt aagtgaaatt
     5941 gtttcttatg aaactcttta attataaaat taagttattc ataggataaa tgtaataaag
     6001 tgtttattat aaacaaaatt caaaataaat aatatctaca tataattaat tttagtgaag
     6061 tatatctagt ttaactagaa tgtatacttc actaatttat ttattcaaaa tatttatgag
     6121 gattttaata tattaactaa aatatatcaa ttgaattaac tttcaaatct ggaaaagcac
     6181 ttgaaatata gtgttttata tcatttaaat ggtcttgaat ttgcttttct gatattttat
     6241 caggtccata tacaaaatac ataatatttt tagtatcttt tgttatagct gttcgattat
     6301 cagggccatt aagaatacta gataatatac caatattatc tgataaaaat aaatcatctt
     6361 ttgttaaaat atgttcttta ccacctatac cataaaaatg ttcacttcca tttgctaatt
     6421 ctattttcaa aggtaattcc attttatcaa ggtcatgacc tgctgttaga agaagatttt
     6481 tcaattctgc taaaaacatt gactcaattg ttacacctac atctgggata ccttttgatt
     6541 ttaatagaat agattctaat tgaagcaata cagggtaagt tttcttgaat tttttataat
     6601 actttatata acttgaaaca ggctctgttt taattttttc tttacgattg tattctgggt
     6661 gttgttctat aatattttta attgtagagt ttttaagaac taaaaaattt tctttattag
     6721 gttttgaatg tgtaatattt ataaccataa ctccaaattt aatattggga taaatttctt
     6781 taactttatg tgatatttct aacataatat agactccttt ttattttata ttttatgata
     6841 taatcttagc aaattaaaag tggattttgt agttccattt ttatagtaat tttatagaac
     6901 cacttttgga ggtattagaa tgtggattac gattaataga aatagtaata tatcattatc
     6961 aagacaaatt tattgtgaga taaaagatat gattctggat ggaagcttaa agtttgagca
     7021 aaaactgcca tcatctagga ctttatcaaa agaactatct gtatctcgta atacagtttt
     7081 agatgcatat aatcaattaa ttgcagaagg atatttggaa acaaggaatg gatatggaac
     7141 aattgtagct aaaggaatat cagggcataa agttgatttt aagttaaatg atacttttag
     7201 agatgaaaaa gatttacata ctaataaaca aaaaaattat atagattttc gttcaggagt
     7261 tccagaatta aaactatttc cacgcaaaga attttcaaag ctatataaac aaatactgta
     7321 tgatttatca gattcggatt tcagatatgg aagtacagct ggtgtatggg ttttaagagt
     7381 ggaaatatct aagtatttat ttcgtactag aggaattaga tgtaatccca aaaacattat
     7441 gattgtttct ggttcaacac aaggtttatc tttaatttca cagcttttat acaaaagtaa
     7501 taaaaaggtt gctgtagaag accctattca taaaggctta ttaaaggtta tttcatctat
     7561 tggatattca gttgttggaa ttaaagtaga tgataaggga atgaatacca acttaataaa
     7621 atcaataaaa gggatttctt ttgtttatac tacaccttct catcaatacc cattaggagg
     7681 gattttacct attcaaagaa gactagagct tattaaatgt gcaacagaga atgattacta
     7741 tataattgaa gatgattatg atagtgaatt tagatatgag ggtcaaccaa ttagttctct
     7801 ttatgaactt agcccaaata aagtaattta cataggttct tttagtaaaa tattatctcc
     7861 agctattcgt gttggattta tgttattacc agacaatatt ttagaaagct ataaaacctt
     7921 aaaaatgtac tctgatgttc atacagaaac aatattgcaa catgtattag ctgaatttat
     7981 tggaaatgga ggtttagaga agtacatatt tagaatgaag aagttgtaca atagaaaacg
     8041 taagtacctc ataaaagttt taaacacgga atttaaaaat gaatttaaaa taaaaggtga
     8101 agcagcaggg cttcatatag ttgctcattt ctataatgtt aaatttacgg aaagtttaat
     8161 tcataaaatt tttgaagcag gtataaaagt ttatcctata caacaatatg caattgagaa
     8221 tatattagaa tatgaccatg aaattatact aggttatgct catcttagtt ttgatgaaat
     8281 tgaaaaaggt gtaaaaatac tcagcaaaat aatacatatg aatcaaggta catagataaa
     8341 acatatagca atctacatat aatagatgta attaagttta aactttaaaa gaaatatata
     8401 ttaatatttt tgtataaaaa tacctgatat ttttttatcg atactttatt gctatacact
     8461 aaattaagat agcaatttat attaagaaaa aactttttgc ataggttata atacttaaaa
     8521 ggagaggata gagttgaaaa caaatacaga tgttattatg gagataattg attatataga
     8581 agaaaatctt acagaaaatc taaatttaga taaaatagct tatattgcta attattcaaa
     8641 ataccattta cacagaatgt ttacaaatgt tgtaggatgt actatccatc aatacataca
     8701 gaggaggaga cttactgaag cagctagaca acttatattt acagacaaat atattataga
     8761 catagctatt acagtaggat attctacaca tcaatctttt acactagcat ttagaaattt
     8821 atataaagaa tctccacaag tatatagaaa taaagatgaa ttttatccta ttcaacagag
     8881 atttgtttta aatctaaatc atcaaattca aagcgaaaaa tgtatttctt atgcaaatat
     8941 gaattataaa caaagaagag atgaaatcaa ttcttatctg aactttaata ttaattgtga
     9001 ggtgaaagta gcatgagttt agtgccacat gtaattgaac aaacaggtca aggggaaagg
     9061 tcttatgata tatattcaaa attattaaaa gatagaatta tatttatagg agaagaaatt
     9121 aatgatgcta ttgcaagttt agtagtagct caattactgt ttttagaatc agaagaccca
     9181 gattcagata taattatata tataaatagc cctggtggct ctgctacatc aggatttgct
     9241 atatatgata ctatgaatta tgtcagatgt gatgtatcga ctatttgtat tggtatggct
     9301 gcaagcatga gtgctttttt acttgcagga ggaacacatg gaaaaagatg tgctttaccc
     9361 aattcagaga ttatgattca tcagccaatg ggaggagcta aaggtcaagc aactgatgta
     9421 aaaatagctg tagataatat attgaaaata aaagaacgat tagataaaat tctttctgaa
     9481 aatacaggta aatcaataga agatattagg agagatacag atagagataa ttttatgact
     9541 gcactagagg ctaaagagta cgggctgatt gattatataa tgaataggaa tgaataaagt
     9601 atcaaagtat catattaaaa tgtctgtttt acaagtatat atataatatt aaataagagt
     9661 gtctcaaaat gaacttttta atttatggga cactcttttt tgtatgaaat taaatatggt
     9721 tttatcattt caacaattat taccaaattt caagtttaat aaattctact ataaaaaata
     9781 cataaaagat aaggttcttt agtaactttc tgtcgtcaaa agtttttata aatttaataa
     9841 caaaacaaga gaaaatacca tatattactt atgtctgcaa tatatagaag acaaaggagt
     9901 tgtacattat gattacaata agtttgtgca tgattgttaa gaatgaggaa gaagttatag
     9961 gcagatgctt agaatgtgta aaggatatag tagacgaaat tataattgta gataccggtt
    10021 ctacagattc cacaaaagaa atagtctcaa aatatacaga catggtgtac gatttcgaat
    10081 ggatagatga tttctctgct gcaagaaatt tttcattttc taaagcttct aaagactata
    10141 ctatgtggct tgatgcagat gacataattt tagaagctga tagagaaaaa ttattaaaga
    10201 ttaaagaaag cttggatact tccattgata ttgttatggc aaaatacaat gtatcctttg
    10261 atgaaaatgg taacccaact ctctcgtatt atagagaacg attgtttaaa agaagttcta
    10321 atcataaatg ggttgaccct attcatgaag tagtacctct ttttggagag gtattttatt
    10381 ctgacattgc aatatctcat aaaaagcttc atagacagga ccctctaaga aatcttagaa
    10441 tttttgaaaa aatgatttca gaaggaaaaa cattggagcc aagacaccaa ttctattatt
    10501 ctagagagtt gtactataat gctaggtata aagaagcaat agaaggtttt actaagtttt
    10561 tagactcatc aagaggatgg attgaggatt gtatcagtgc ttgcagagat ttagccacat
    10621 gttattactt aattaatgat gaaaaaagtg cattatattc attgtttaga agtttcgaat
    10681 ttgatgagcc aagagcagaa atttgttgtg acataggaaa gcatatgttt gatagacaaa
    10741 aatataaaga agccattttt tggtataagg tagcattaac aagagataag aatgatacta
    10801 atggaggttt taagtcaaat gattgttatg gatatatacc atctatacaa ttaagtgtct
    10861 gttatgacag gttaggagaa tcagataaag ctatttatta tcatgaaaaa acaaaagaaa
    10921 tcaaacctaa cgatagtgct gttttgcaca atgaaaatta ttttagtaaa tttaaaaata
    10981 gttgataggt cacaatcata ctaaatttga catattatat atagagctta aaagctcaaa
    11041 tatatcagga ggtgctttta ataatgagta gaaataaata ttttggacca tttgatgata
    11101 atgattacaa caatggctat gataaatatg atgattgtaa caatggtcgt gatgattata
    11161 atagctgtga ttgccatcat tgctgtccac catcatgtgt aggtccaaca ggcccaatgg
    11221 gtccaagagg tagaaccggc ccaacaggtc caacgggtcc aacaggtcca ggagtagggg
    11281 caacaggccc aacaggacca accggtccga ctggcccaac aggaaataca gggaatacag
    11341 gagcaacagg attaagaggt ccaacaggag caacaggggc aacaggccca acaggagcga
    11401 caggagctat agggtttgga gtaacaggtc caacaggccc aacaggagcg acaggagcaa
    11461 caggagcaga tggagtaaca ggtccaacag gtccaacggg agcaacagga gcagatggaa
    11521 taacaggtcc aacaggagca acaggggcaa caggatttgg agtaacaggt ccaacaggcc
    11581 caacaggagc aacaggagta ggagtaacag gagcaacagg attaataggt ccaacaggag
    11641 caacaggaac acctggagca acaggtccaa caggggcaat aggagcaaca ggaataggaa
    11701 taacaggtcc aacaggagca acaggagcaa caggggcaga tggagcaaca ggagtaacag
    11761 gcccaacagg cccaacaggg gcaacaggag cagatggagt aacaggccca acaggagcaa
    11821 caggagcaac aggaatagga ataacaggcc caacaggtcc aacaggagca acaggaatag
    11881 gaataacagg ggcaacagga ttaataggtc caacaggggc aacaggaaca cctggagca
//
LOCUS       DAKBNN010000122        11850 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000122 DAKBNN010000000
VERSION     DAKBNN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.123"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            315..836
                     /locus_tag="OUB02_002615"
     CDS             315..836
                     /locus_tag="OUB02_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8886360.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1215..2390
                     /locus_tag="OUB02_002616"
     CDS             1215..2390
                     /locus_tag="OUB02_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886361.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2424..3962
                     /locus_tag="OUB02_002617"
     CDS             2424..3962
                     /locus_tag="OUB02_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCT8886362.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3994..5196
                     /locus_tag="OUB02_002618"
     CDS             3994..5196
                     /locus_tag="OUB02_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8886363.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDSHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGEGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5363..5962)
                     /locus_tag="OUB02_002619"
     CDS             complement(5363..5962)
                     /locus_tag="OUB02_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8886364.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            6132..7490
                     /locus_tag="OUB02_002620"
     CDS             6132..7490
                     /locus_tag="OUB02_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886365.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEKLFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            7483..8115
                     /locus_tag="OUB02_002621"
     CDS             7483..8115
                     /locus_tag="OUB02_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCT8886366.1"
                     /translation="MNNKYIRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDIDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            8444..9601
                     /locus_tag="OUB02_002622"
     CDS             8444..9601
                     /locus_tag="OUB02_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8886367.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(9748..10383)
                     /gene="lexA"
                     /locus_tag="OUB02_002623"
     CDS             complement(9748..10383)
                     /gene="lexA"
                     /locus_tag="OUB02_002623"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCT8886368.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(10487..11614)
                     /locus_tag="OUB02_002624"
     CDS             complement(10487..11614)
                     /locus_tag="OUB02_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8886369.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         4451 a         1456 c         1914 g         4029 t
ORIGIN      
        1 tttggataac caatactatt ttactgataa tcttttcctt atcactaatc ttacatttta
       61 aaatattatt tttactcgta atattatcat aagaatttgt ttcttattta aaactaatta
      121 gaattataaa gtttattgcc atgttattaa taaaataaat ccagttaaat cgagtgctag
      181 ttaaagtaat aagaaacttt tacaatggtt tcctttatcg attgaaataa aagttaatat
      241 ggtataatat ctaaagagag aatcttatca attagaatat gcatagaaac aagtggctta
      301 aaaggagacg ttatatggaa gagatagtaa agaaggaaca agcaagtatg aatgatattt
      361 ttttgtatct taaagctaaa tcaaactcat tatataaatt tgtaatatta tataatgagt
      421 ttatcaataa acctaaagat tatggaactg gggaacttat aaacatggtt gaagtacata
      481 tgctaacagc aattgaagaa aatccaggaa taagtccaac tcagttagct aaaatatgga
      541 acagaactaa aggtgcaata tctcaaacta tatctaaatt ggagaaaaag ggttatataa
      601 ctaaagaaaa gaaagaagga aatgcaaaaa ctatattact atatccaacc acatcaggag
      661 tagagttaag taaggcacat aaactatatg acaccataga tgttacaaga acatttgaat
      721 acttaatcaa ggagtgtaca ttagaggaga tagatagttt ttataaagta attgatttgt
      781 ttattaattt attagaagaa tctccgcagg aaaataatga ttctgaaaaa gtgtagctga
      841 gattgaagtt gaataaagag aaatagatat tatagaagta gtacttaagt acacattata
      901 taaaaatata ataggtatag gattaaaaag tttagtggtt taaagtttaa ccactaaact
      961 tttttaatgt ctataatcta ctattattta atttactaaa aataaaaaaa ctcattatat
     1021 taagataaaa atagaaaaat aactagaaaa atactataat atatcagttt aatacagaaa
     1081 aaagttaaaa aatttgagaa aaataaaaat gaaaacgttt tatattgaca tagagaaaaa
     1141 gtagtgatac catttaaata tagtttagta gctaaactat atttaaatta taataactat
     1201 tttaggggga tgttatgaat ttaaaacaat taagtgaaaa atacgaagaa tatgtaattc
     1261 aaatgagaag agaatttcat aagtatccag aattatcttt taaagaaata agaacaaccc
     1321 aaagaataag ggaagaatta caaaaaatag gggtcccttt tgtatctatt gaaccaaata
     1381 tcgtaatagc tactatacaa ggcaaaaatg aaggtaagac aattgcaatt agaggagata
     1441 tagatgcgct tccaatgcaa gaagattcag atgttgaata caaatcagaa tatgatggaa
     1501 taatgcattc ttgtggacat gatgctcatg gagctatgtt actaggactt gcacatatgc
     1561 taaatgatat aagaaaagat ttaaaaggta gagtattatt agtttttcaa gcagctgaag
     1621 aagttggtgg tggacacaag gagataatac agtactttaa ggaaaatgga ggaccagata
     1681 gattaatagc tactcatgtt tggtccgata ttgaagctgg taaaatttct gttgaaccag
     1741 gacctcgtat ggctggtggg tctggttgga gaatagacat aacaggtaga ggaggtcatg
     1801 gttctagacc agatcaaagc atagatccta taaagccagc ttgtgatata gttttaaaag
     1861 tatcttctat accagttaat catataagtg tactagaaca atgtgttgta catgcatgtg
     1921 caatacatgg aggaactaca ataggaaatg ttattccaaa caatgcagag gtaataggtg
     1981 gatatagata ttttactgaa gaatcaggca aaaaagtgtt tgacattata aagcaaatga
     2041 gtaatggtgt tggacaagtt tatggagtgg atgtgaaggt tactcatact ggaggcatag
     2101 gacctgttat caatgatgaa gaagcagtta agatggctaa agaaatagtt tctaatatag
     2161 atggattgga gttagattct tatgagccaa tatgtgcatc agattgttat ggtgaaatat
     2221 taaaagagta ttctggcttt tattgttatc taggggtagg taacaaagaa aaaggaataa
     2281 tatatgcaca acaccatcct aagtacaata tagatgaaga tacattaaag ttaggttgcg
     2341 aatttatggc taaatatgca gttgaatttt taaataaaaa taataactaa aataaatcga
     2401 attttataaa tggggggatg aaaatgcaag gtgcattaaa ggaaaataag aaaaaaagtg
     2461 gtggaggatt tttaggatgg attgaaaaag ttgggaataa aatgccacat cctctagcat
     2521 tatttacata tattacgttg atagtagtag cattatctgc aatagcaagc tttttaggtt
     2581 tttcagcaac aagtcctgct acaggaaagt taataaaagt tatgaactta ataagtgcaa
     2641 aaggtttagt actatttatg caagagtttg ttaagaactt ccaaaacttc cctgtgcttg
     2701 gagtagtttt agttatggca gttgccacag gagtttgtga aaagacagga tttttttcaa
     2761 ctgccataaa aatgagttta tcaaaagtta aggggaatgc ggttgtattt ataatagctt
     2821 ttataggagt atttgctaat caagctggtg atgctgcttt tgtattagta ccaactcttg
     2881 caggagcaat attttatgga ttaaatagaa atccattagc tggtatattc tgtggatttg
     2941 catcagttgg tggtggattt gcaacagctg taattcctgg gggatgggac gttacgctaa
     3001 cgccaataac tgtatcagct gctcaaacta ttcaacctgc atttgatatg acattattag
     3061 ctagttatta tgcactattt gtatctgcgt ttatagtagc tacagtatca acaatagtaa
     3121 cggttaaatt tatagaacct aaattaggta agtatactgg taaaccagaa ggtatagatg
     3181 ataatcaagg ttttgaggtc acaaaagaac aagcaaaagc agcaaagtta gcaggtttta
     3241 cagttttagc ttatgtagcg ttattaattg ctttgtgtat accagctaat agttttttaa
     3301 gaagtccaga aggttcttta atatttggag caccacttat gtcttgctta cagtttttta
     3361 tagtaatact attcttcttg ccagggattg tgtatggaat gagggttggt aaaatcaaga
     3421 ctgtaaaaga cttaaatgat atattatgtc aatcaatggc agcaatggca ccgtttatcg
     3481 tacttgcaat agttataggt caattcttaa cgctattctc agtgtctaat ttagcacagg
     3541 tactagctat aaaaggtgga gcattactca actcattgcc actaccacca caagctataa
     3601 tactgctatt cttagtacta gtagcgttta taaatatatt tgttataagc ggaagtacaa
     3661 aatggatgat atttggacca gtatttgttc caatgttaat gcaattaaac atacatccag
     3721 catttactca gtttgtatat agattaggag attctgtaac caatcattta tcaccattaa
     3781 atgcattctt tataatactt ttagcaacag tccaaaaata cgataaaaat gcaggtatgg
     3841 gaactatatt ctcagctatg ataccttaca ctataggtta ctttgtagta cttgcagttt
     3901 tagtagtagt ttggatgaca ataggtatac cagtaggaat aggtggacaa gtttggttat
     3961 aaaacatatt taataatttt gggaggaaag aaaatggaaa caactgaaat aagatacaat
     4021 tttgatgaag taatagatag aagtggaaca aactgtctta aatatgatct taagagtcaa
     4081 tttatgccag aatctccaga agatgcacta ccactatggg tagcagatat ggattttgca
     4141 tgtgcccctg agataattaa tgcaatgcat gaaagaatag acagaaaaat atttggatat
     4201 agctctcaac aaactgatga ttattatgaa gctgtatgtg gatggtttaa gagaagattt
     4261 gattggaaaa tagaaaaaga gaatatattc tttagcccag gggttgtgcc tgcgttagga
     4321 tttttcacac aaatattaac acaaaagggt gatggtatca taatagaaaa gcctgtatac
     4381 tatccttttg aagataaaat aactaacaat gaaagaaaag tagttaatag tcctttaaag
     4441 tttgaagata gtcattattc tatggattat gatggatttg aagaattagc taaagatgaa
     4501 aataataagg tgtttatatt atgtagtcct cataatccag ttggtagagt atggaaaaaa
     4561 gaagagctta gaaaaatagt agacatatgt aagaaatatg atttgtggat aatatctgat
     4621 gaaatacatt gtgatattat tcgtaaaggt gaaaagcata taccattaga agtagcatgt
     4681 cctgattata aagatagaat aataacttgt acagctccaa gtaagagttt taacttagct
     4741 ggtatgcaat tatcaaatat agtaatcaat aataaggaat tacaagaaaa atggatgctt
     4801 gaaacagatt ctaaattagc tatattccta cctaatccat tcgcaatagt tgctactaaa
     4861 gttgcttata atgattgtga agattggatg aatcaagtaa atgagtatat agatggtaat
     4921 attaaatatg tagaagattt tgttaatgag tatatgccta aagtaaagat aataaaatct
     4981 caaggaactt acttagtttg gttagacttt agagggtatg gattagatgc aaaacagctt
     5041 gaatatgtaa tgcttaacaa ggcgaaggta ttgctagatg aaggatacat attcggagaa
     5101 gaaggagaag gatttgaaag aataaatgtt gcttcaccta gaacaattat agaagagtgt
     5161 atgaatagaa taaagtgtgc atttgaagga ttataatagt aatatctaaa taatatattt
     5221 gtgtatttta aaaaaagact atgctaaaaa aatatttcaa agcatagtct tttaaagttt
     5281 tatgatgagt ttaaaaaatt tcaaaacgtt actattcact tacatgttta tattagaaat
     5341 aattccacaa tatactcatt ttctaaagtt gcacttttat acaccttgct tcaccaatat
     5401 catgtgtaac caaaatagta gacttattct cattcttaat aatactataa acatcatcac
     5461 aaactaataa tctagtctga taatcaagag ctgaaaaagg ttcatctaaa agtagaatat
     5521 ctggatttac agaaagtgtt ctaataagcg caactctttg tctcatacct ccagataatt
     5581 cttttggata catacttcta aaatcccata atccataagt ctttaaaagg ctttcaactc
     5641 tatctaaaga ttttttatct tttttgcctt gaatttccaa tccaatagtt atattatcca
     5701 ttatatttct ccattcaagc aaattatctt tttgaaacat atatccaata tttccagtaa
     5761 tctttatatc accactagtc ggttttaaaa gattagtaag aatatttaat attgtagatt
     5821 taccacttcc agaaggacca agcaaagtta atatttctcc ttctttcaag ttaaaattga
     5881 catcctttaa tacttcaagc tcgccttcct tagtgtagaa gttcatattt atatttttaa
     5941 cttctactat gttgtcattc attatgccac cccctataac ttttagctcg tatatattat
     6001 gttattaaca atatgaccta tttgtaccat ttttcataaa tataatataa atattttgtt
     6061 tatttacgta ttttcttagt aacttgtacg tataaattat agataaataa attataaggt
     6121 aggggataaa tttgagtaac ataataacga atttaataga gtcaatattt actaatccaa
     6181 taactatatt gtttttactt gcaataactg tgtattctgt ttttaaaata atagaaaata
     6241 acagagtata ttcatttgta agttcaaaat tagacgaaat caatagagac tataagacaa
     6301 gtgagtttta taaaaaggta agggatgact actatacata tagtaaagaa aatccttatg
     6361 cagacgtaaa tataacttca tttatagagg aagttgtatc tgatttaaag cataataatt
     6421 tgccattgtt agaaaaaatt agaagtataa aaaattcatc ttcaataagt atattacttg
     6481 gggttttagg tacgtttgtt ggtctttcta caatgctttt atgtgttgat actaaagaca
     6541 ttataaattc acttccttca actattagta gtatgcaaac tgcatttaca actagtatat
     6601 ttggagttgt attttctcta attataggtt actttactaa gataaaagat tgtgaacatg
     6661 ttttgataca aataatgtta aaatctgaaa atttactaac atctgaaata actcatttta
     6721 agtcagaaag aatggattta aaagtagaag aggtaaaaaa tacaataaaa caaataagta
     6781 agtctataga agctattgaa aaatttgata aaatatctaa agatttaaat gactttaatg
     6841 atgaatttat cagtggtata gaagctttaa aaagcctttt agaaggttct caaagttcta
     6901 ttaagacatt tgaccagagc gtaagaaaat tagataaaca gtttaatatt ttaaatataa
     6961 aatttgtaaa gctttttgat aaatatgata atcaagataa tataaataaa gagattttat
     7021 ttgatataaa agaatcttca aaaaatatat acaatgccac tgaaagccaa ttcaaaatta
     7081 gagattatat aaaaaatata aatgcaggat ttgctctata tgaaagaagt gctcaagact
     7141 tattaactaa attaatgacc catgaaaata aaatctctca aaatcaaaaa atccttttag
     7201 atgaaaagtt taccttagat gactcaataa aaaatctctc aagtattatt gaaaactttt
     7261 ctaatgacct acaagttaaa ctggatatga tgtttgaaaa ttctttagat attcaagata
     7321 agttagatgt tatgtttaat aactctttta tgaatgatga agttcctctt gatagtgaaa
     7381 aattatttaa ggatgatgtt aataatgtat ttaatccttt tagtgaagaa atttatgaga
     7441 tagaggataa agaaataaag gtcataggtg aggatgagtt aaatgaataa taaatatata
     7501 agaactgtta acagggaatt tgaaaagagt agcttttggc caacatttac agatttatta
     7561 tctactgtat tgatggttgt aattcttatc ttatttagct cagaaagtat ttcaggttct
     7621 gttgaacaag atttagctaa gaatgtcaat gcttctgttg aagaaacttt taaaaaaagt
     7681 ggaatacctg tcaaggtaga taaatctaat gggcaagtaa cttttggtga aaaaaccatg
     7741 tttgatatag atagtgatgt tttaaaacca gaggctaaag aaatgctaaa aatgtttgtt
     7801 ccaaagtaca tagagactat ttataaagat tatggtgact atatatccaa aatagttata
     7861 gaaggtcata cagatgatgt gggaagttat atatacaact tagatttatc gcaaagaaga
     7921 gcatatagcg ttgctaaatt tatagtggga gatgaaatag gtgactataa atacaaagat
     7981 aaagtgacta agcatattat tgcaatagga aggtcaaaag ctgaactcat caaaaatggt
     8041 gacaatagcg tgaatagaga tgcatctaga agagtagaac taaaatacga aataaatatt
     8101 aatcaaaata aataattaaa tttttataaa aaaaaagaag gcagttggtg tgagctttaa
     8161 gccttcttta aggaaaaagt ttatttattt ttgttgatac ttaacatatt atagttttta
     8221 gaagcagttt gcaatatatt aaacgtaaat ggtcgtttaa aatgcataac tggatactac
     8281 tgatgtaaaa tatttcattt tatataatat aaaaattata cagtgtcaat tttctatatt
     8341 attgtactat aatctattat ttaaaatatt atgtaaaaaa atatcataaa aacgcatatc
     8401 atgttataat tactagagta ttatgaatat ggaggcgtag tttgtgatag aaataagtat
     8461 aaaaaataaa aaaaattcta aaattaaaac tttgatactt ttattgatag tatctatatc
     8521 gatttattta actggttgta ctacatcaga taatggaaat aatcaatcaa ataataatga
     8581 ttctttgtct agtagtatat caagtgataa gaatgtaaaa attcatttta ttgacactgg
     8641 aaatagtgat gcaatattga ttcaagatgg taaaactttt acattgatag atgggggaga
     8701 taatgatgat gagggattaa tggtagatta cctaaacaat caaggtgtaa aagatattaa
     8761 atatcttata gctacccatt ctcatgcaga ccatttaggt ggacttgatt ctgttgtcaa
     8821 aaactttaat atagaaaatg tttttgtatc gaatggttca gcagaaacaa agagttatag
     8881 ggactttata aatgctttag caaataaggg tttatcccca agtgtaccgc ttgaaaataa
     8941 taagttctat ttagaagatt cttattttga agttctaaat acaaatgggg gagatactac
     9001 taatgaacaa tctctagtcc ttgtatatac taatgggaat gataaagttt tatttacagg
     9061 tgatgctgaa gaaggtaccg aaaaagaaat actacctaag cttgagaaag tagacctttt
     9121 aaaagtagct catcatggtt ctcgtagtag ttcatctcaa gagtttttag ataaagtaaa
     9181 tcctgaatat gcggttcttt tagtaggcaa gggtaattca tatggtcatc cacatcagga
     9241 gacaatgaat aaattaaaaa aaatgggagt aaaagtacat agaagtgatg agtgttcaga
     9301 tataatattt gaatcaacag gtgatggagt atttacaagt tgtaaagatg gaagttataa
     9361 taaaggcgtt agagaagatg gaaactctgg aaaatcaaac agtaatacaa gtaaaaatga
     9421 ttcttttaaa aataaacaaa atacatccaa ttcagaagaa atagtgtatt ttactccaaa
     9481 aggtaagagt tatcactcta caaaaaattg tagtgggctt tcaagaagta aaaaaatatt
     9541 atcaggcaca attgcagaaa gtaagaaaaa tgacccatgt gatatatgtt atggaaaata
     9601 acaaaaaaac aaccaattta tctataaagc ttctgaactt taaatcattt aaaaaataaa
     9661 ataaatgcat aaaaaaataa gctataatca atagataaca cattttatct atacgaatag
     9721 cttatttttt taagtatttt ttaatagcta tttaataact ctaaatacac cagttacaat
     9781 acctacaatt tcaacttctg agtcattgag cataattggt tccataaaat cattttctgg
     9841 ctgaagtctt attaaatttc cttctttaaa aaacctctta accgtagcag attcaccatt
     9901 tataagtgca actactatct gagaatttaa ggctgtattt tttttgtcta ctacaacgta
     9961 atcttcatct aaaattcctg cattaatcat actttcgcct tttactctta atacaaaatt
    10021 gttactgccc tttactaaac tggcagggaa tggaatgtat tcctctatgt tttgttgagc
    10081 taaaatgggt tctccagctg ttatttgacc tactaaagga agctctatta tttcttgatt
    10141 agctccagaa acatcattta ctttactacg ttctaaaact tcaatagctc ttggtttagt
    10201 agggtccttt cttatgtatc ccagtttttc aagtttattt agatgagagt gcacagttga
    10261 agtagatctt aaccctacag cagtacatat ttctcttaca gcaggtggat aaccttttaa
    10321 tatgatttga gactttataa attccaatat caagacttgc ttttcagtta gatctaaata
    10381 cataacatca ctcatttcca aacatatttt gtttttattt ccatacactt tatattttta
    10441 attataacat aatccattta tatctgcaaa catttgttcc tgatttttaa tcgtcctcaa
    10501 tccattcaaa attcttgact atatcacgtt ttacattttt cttatgagct gcataaagct
    10561 gagtagttgt gacattatca tggtcaagta agttttgaac atcatatata gaaaatcctg
    10621 tttggattag agaagttgca gccgttgctc ttaatttatg gggactatat ccattgtctc
    10681 tagtagtatc aagtggaata gatgtatatt tttttaccag tgttcttata gcctttgctg
    10741 taattctctt attttgaaga gacaaaaata aagcatcttt ttcttcatca tttaatagtt
    10801 catctcttgt tctttcgttt tgcaaataat cttttattac atgttcacaa gtatgattta
    10861 taggcattaa aacttctttc ccacgttttc tatatatttt aaattcacct cttgaaaaat
    10921 taaaagagga gatattgagt tctctaagct catttaatct aagcccgtat gtaacaaata
    10981 atgctagtat agctttgtca cgtaatttag tttttctcca atataccttt tctttctctg
    11041 taagcccttc acctgtttct actgattcaa gcatcttagc aacttcatct atctcaaggc
    11101 gcttaatagc atctggctga ggtttaggaa gttttattgg attgaatcca tcagttatat
    11161 tattatctat ctgagagttt ctgtataaga atttaaatag agtagagatg gatgattttt
    11221 ttcttgctaa agctctattg ttattttcaa aaatcaatgt gtttttttct gtatttttgt
    11281 aatatctact gcaataatca cctaagaata aatttatatc tcttgatttt attgtattaa
    11341 aatcatcttc tgtaatgtct tttacacaat ctgcatttgt taattctttg gtttcaatca
    11401 aatatgaaca aaaaaaatta atatcttcta aataagcaag tcgtgtagaa actgcaacag
    11461 aaccttttaa atatataaaa taatccttca taaatacagg aagtttattt tccaaaagta
    11521 gacatttttc tatgagttca ttttctttga taacaatatt atcaggttta tctgatttat
    11581 tatgtacttt tataattttc ttatcattat tcaaaattaa acctcctaaa actaaaatat
    11641 aaatctttta tattattttt tgatatttat accaagtgag tataaatatt taatttaatc
    11701 attttttaca tttttaagag attagcagtg ctttagatga aatcatcact aactattccc
    11761 taaatccaca tttaaagact agttgaattt aagtactctt ttttatttct caatatattt
    11821 atagaaatat tttaaaatat attgagaaat
//
LOCUS       DAKBNN010000123        11726 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000123 DAKBNN010000000
VERSION     DAKBNN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.124"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(248..1075)
                     /locus_tag="OUB02_002625"
     CDS             complement(248..1075)
                     /locus_tag="OUB02_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8886370.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1123..2559)
                     /locus_tag="OUB02_002626"
     CDS             complement(1123..2559)
                     /locus_tag="OUB02_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HCT8886371.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2646..3515)
                     /locus_tag="OUB02_002627"
     CDS             complement(2646..3515)
                     /locus_tag="OUB02_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCT8886372.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3673..4326)
                     /gene="phoU"
                     /locus_tag="OUB02_002628"
     CDS             complement(3673..4326)
                     /gene="phoU"
                     /locus_tag="OUB02_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCT8886373.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4354..5118)
                     /gene="pstB"
                     /locus_tag="OUB02_002629"
     CDS             complement(4354..5118)
                     /gene="pstB"
                     /locus_tag="OUB02_002629"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCT8886374.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5137..5961)
                     /gene="pstA"
                     /locus_tag="OUB02_002630"
     CDS             complement(5137..5961)
                     /gene="pstA"
                     /locus_tag="OUB02_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCT8886375.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVTGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5963..6880)
                     /gene="pstC"
                     /locus_tag="OUB02_002631"
     CDS             complement(5963..6880)
                     /gene="pstC"
                     /locus_tag="OUB02_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCT8886376.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7261..7707)
                     /locus_tag="OUB02_002632"
     CDS             complement(7261..7707)
                     /locus_tag="OUB02_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886377.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            complement(8224..8904)
                     /locus_tag="OUB02_002633"
     CDS             complement(8224..8904)
                     /locus_tag="OUB02_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886378.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(8954..10969)
                     /locus_tag="OUB02_002634"
     CDS             complement(8954..10969)
                     /locus_tag="OUB02_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCT8886379.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILFPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(11041..11717)
                     /locus_tag="OUB02_002635"
                     /pseudo
     CDS             complement(11041..11717)
                     /locus_tag="OUB02_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         3793 a         2107 c         1208 g         4618 t
ORIGIN      
        1 tattttttct gaattttctt aattatgtct attctctttt atatagttgt tatattttaa
       61 aatatgtttt tacagttatt ctgtcttata atgtcaataa ttgcaagatt attttatttt
      121 aattttaatg ctttctcatt aattttatat tatattatcc ttacagtata cctcttattc
      181 gtatttttta tattatatgt tttattttat aaataattta ctttatcttt tactatattt
      241 aaatttatta taagttttct tgccagaaat ttttaggtgt tatattttct gtaattggca
      301 atattgtata tcctctctct tttaagattc ttataatatt atttaaagca tttaaacttt
      361 gctctttctc atgaattaat actactaatt catctctttt tcttccataa tataatatat
      421 tacttactat ctgacttgta gaagacttcc aatcctgtgt atccaaattc cagtcccaaa
      481 ttaaataatc atttgccata agtttattat atgaaccttc tggcatatat ggtttacttc
      541 cgtatggtaa tcttattaat tttgaactct gaccagttat ttcttgtaga gtattattgc
      601 atgtattaaa ttccccaact gttacttctg ggcttttata taacttatgt atatcatgac
      661 ttacactatg aaaacctatt gagttttctt cttccaagac tctccttact tcttccttgt
      721 ggtttttcat attctgatta agcatgaaaa atgtcgcttt gacatcattt tcttttaaaa
      781 tatcaagtag tgcatttgta tattttgatg gaccatcatc tattgttatg tatgctactt
      841 tttcagaagt agcccctgga ccttttgttt cttcttgaga tgctttgtat atgatttctc
      901 ctaaagtatt actttgagaa agcagttcca gtgtattttc accttttgaa cttatatttt
      961 caactggaac attattaatt ttttcctcca ataatgttcc agttaaaatt aaaacagcta
     1021 taataaatat ccttataaat acatgaaaaa cagccttatt atctttaatt ttcatccccc
     1081 atatctccct tttataaaag cataagttta agacttaaaa agttattttt tatatttatt
     1141 ctctttctta ttacttttta tatctctact aaatattttt ttaacctttt cttcttcttt
     1201 ttctcctaaa gattctactc ttttgattcc caaagaagtt aaaaattgta atacatttaa
     1261 attatcaaat ggagtttcca tattcgtact ttcactatat tcatcaacat tcttagtacc
     1321 ttcttccaca cttaaaatac gcttcggacc tcctacacag cctcctacac accccattcc
     1381 ttctataaat gtagcttcta tttctttatt tagaactttc tttagaccgt ccatgcactc
     1441 ttttgttcct tcaaatgcct tttcttcaaa ttttatattt ttgtcaattc ttctaactga
     1501 taacttaact gctttactta cacctcctgt tctagcataa actctacctg aaagggacgc
     1561 ctctactcta ttactctcat ccattattgc tggatttaac tctaatgcat caaatatttc
     1621 ttgcaactct ttaaatgtaa gcacaaaatc aactgcatct tttatatctt ctaacattgc
     1681 ctctttcttt tttgccatac aaggacctat gaatacaact ttagcatctg gatttaaaat
     1741 ttttacagct ctcccacaag caatcatagg agaaacggat ggtgatacat tttccaatat
     1801 ctgaggaaaa ctattttgta tcaaactaac ccaaatagga cagcaacaac tagttataaa
     1861 gcatccttct tcgtcctttt tcatatgctc ataataatca tatgcttctt ttgcagtaag
     1921 catatctgct gccaaagcaa cttctatcat atcttcaaat cctatctttt ttaatgcagt
     1981 tcttaattta ccagggctta catctttccc aaactgtcct acaaaagctg gtgcaactat
     2041 tgcatataca gggctcttta tatcttttaa caattttatc attggtataa attctatttt
     2101 atctgaaatt gcacccaact tacatcttga tacacaatca ccacaagcac tacatacatc
     2161 ttcctcatta caattaatat gcttacttct tatcttacat gactcatctt ccttattatt
     2221 ttcatcacaa tatttgcaag catctgatat tacagatatt attggtcttt tgacttcatc
     2281 taaatttaca gcttcatgta gtgactcttt taaattttta tctgtatctt ctgtcacatc
     2341 caatcccata gttaccctaa ttaaatttac catagtatct ttatcaaatt ttccactaaa
     2401 ataattattt aaattatcct ttaaatcttc atttaaaatt ttattaggta agttttcaat
     2461 atcctctata tcatcattcc acactgcttt tacaagttcg ccaaaaagct tcattttata
     2521 tatttctagt attttagaat tgtcatttct agtattcata aaaaatagta cctccataaa
     2581 ttaaacttat atatgtttaa tatttacaaa gatactattt tttatttatt atttaactac
     2641 tattattaat atgatttatt tagctttctc aacaactcta tttcaatcat tattttcatt
     2701 agatgatagt tgaatacagg gcttttttct tcatctatat tccagtctag cttatccaca
     2761 ttatatgttt tcttaaggta tttatatgta tgctctttca aataaagttt attttcaagt
     2821 aactctatag actgcatatg aaaatgtatt tctctacata tccttaaggc attctcaatt
     2881 ttttctatat tagtttcact caaactataa tccaactcat caataagttt tgaatatatt
     2941 tcttctaatt tttctacttc ttcttcaaat ttctctacat ttaaattttc ttttctaaga
     3001 attttatctt caataagcaa cattgaaagt tcttctatct ttttaaactc atctataaca
     3061 ctatttagat aatttggtct agcaacataa taattaacaa gtactccaaa caaaacaccc
     3121 acagatgtat ctaacaccct atgtaaagaa taatacacag ggtcactatt tcctacacct
     3181 agatgtatag ccagaaaggt tacacaggca actactactg actttatttt aaacagattg
     3241 cataaatata ttgagcacat tactcctaga ccacataaaa atgggtctcc agctttaatg
     3301 attgcaaata tgaatcctat tataccacct aaaatagtac cttccactct attgattccc
     3361 gtctttaaag aaccttttac tgtatcttga acagaaatta tacatgctac cagagaataa
     3421 aacatattct ctactaaata gtcaccaaga atcatacata aagtgacact aattcctgtt
     3481 tttatagtcc tcatacccat cttttgaagt ttcaaagttc cacctcctaa aaagcacctt
     3541 atataaaata cgcatcctca tatttaatat gttcctagta tatcacaatt ttttaaattt
     3601 acccattaat catatttttt tatcaattct ttcttaattt agacaataaa aaaactgctt
     3661 taaagcagtt ttttatccta tctcaaccat tttaccctca attgcaaaaa ttatcttctc
     3721 acatacattt gttatatggt ctccaattct ttcaaggtat cttcctataa gtagaagttt
     3781 tacaccttgg tctatggtat ttgagttttc atgcattatt tttaaacaat caatttgtac
     3841 ttgttcatac aatccatcta caatatcatc tttagttcca acatcataag ctatacttgc
     3901 atcaaataag attaaagctt ctctagtctg agacatcata gacttacatt cttttgccat
     3961 cttaggaata tctattagct cttttatata agcctctcca cctatcttca acatttctct
     4021 agctatattt gctgcataat ctcctattct ttccagctct atagatatat tacctaatga
     4081 ataaataaat cttaagtccc ttgccattgg ttgctttaat actattagct ctatactctt
     4141 atctcttatt tcttctctta atttatttat ttcttcatca tcttctacta cttttcttgc
     4201 accctctaaa tccttctcta tcatacaagt cacagacttt tctattgctt cttcacattt
     4261 ttccatcata agcactgttt gttcttttaa agtatcaata cttatatcta aactattatt
     4321 gaccattgtc taactccttt atagttatat attttaacca aatctacctg ttatatagtc
     4381 ttctgttctt ttatctacag gagtggtaaa catagttttt gtatcgctaa attctataac
     4441 ctctccattt aagaaaaaag ctgtttcatc tgatatacga gcagcttgtt gcatattatg
     4501 tgtaactatg actattgtgt agtctttttt taaatcctct ataagttcct ctacttttaa
     4561 tgttgaaatt gggtctaaag cagatgtagg ttcatccata agaataactt ctggtctcat
     4621 agcaattgct cttgctatac atatacgctg ttgttgacca ccagaaagtc ctagtgcaga
     4681 tgatttaagc ctgtctttaa cctcatccca aatagctgca ccttttaagc tttcttctac
     4741 aattttatct aattgcttct tatctcttag tccatgtgta cgaggaccat atgccacatt
     4801 atcatatata ctcataggaa atggatttgg cttttgaaat accattccta ctttagtacg
     4861 aagatttata acatcatctg aggtatatat gtcctctcca tcaacagaaa tgtttccttt
     4921 aattgttaca tcttcgatta agtcattcat tctatttaac gttcttataa aagttgattt
     4981 tccgcatcca gatggaccta ttaaagcagt aaccttattc tcttttatat ccatatttat
     5041 tttctttaat gcttgcttat caccataaaa taaatccaaa tcttttacac tcatttttat
     5101 cttatcgatt aattccatta ttcacacctc ttaaatttaa taattagctt tatttaattt
     5161 cttagcaaca aatcttgcta gcatgtttag tgctaataca attactatta aaacaattcc
     5221 tatactggct gctgttgcta tatctccact ctctttagtc agtaagtatg agtgtacagt
     5281 taatgttctt gcactatcaa atattccacc tggcatcttg gctacagtac ctgctgtaag
     5341 taatattgca gctgattctc caattatacg accaactgat aagattactc cagataaaat
     5401 tcctggtatt gcacttggaa gtactacctt atataaagtt tgaaattttg tagctcctaa
     5461 tgccaatgat gattctctat aagcttgagg aactgttttt aaagcttctt ctgttgtacg
     5521 aattattact ggtaatatta ttattgcaac tgtaagtgaa cctgtaacta tggaatatcc
     5581 catttttaat gtaactacaa agaaaattcc tccaaataat ccatatacta tagatggtat
     5641 ccctgataaa ctttcagttg caaatcttat agctgtaact gcttttcctt ttttagcata
     5701 ttcttgtaaa tatatagcag ccaatattcc tataggagtt gctacaacta tagaaaccac
     5761 tactgtatat aatgttgtaa ttatcattgg aagtatacca ccatcacctg atgctgaata
     5821 attgctaaat aagaaattaa aattcatgtt gccaattccc tttataaata taaatcccac
     5881 tataactact aatgatacta ttgtaaatag tgctgataga tatactaatg acttcaatag
     5941 attctctttg aactttctca tcttaattta ccgccttatt tgaaagttta tttaaaacta
     6001 agtttaaaat taatataaat gtaaacaata ctactccagt tgcaaatagc atttcttgat
     6061 gtgtaccaaa tgcgtatccc atttcaagtg ctatattagt tgtaagtggt cttacactat
     6121 ctgttattgc agttggcata actggtgagt tgcctgcaac aagtattact gccattgttt
     6181 cacctaaagc tcttcctact cctaacacta ctgcggctaa tactccagat tttgcagcag
     6241 gtaatacaac tttgaatatt gtttctattt ttgatgcacc aagtgctaat gaaccttctt
     6301 tgtatgcttt tggtactgct cttattgctg tttctgatac agatattatt gttggtaaca
     6361 tcattactgc taatactatt actattgcta acaaactttg accttttgga agattaaata
     6421 tactttgtat agttggaact atcactgcaa gaccaaatac accatataat actgaaggta
     6481 tacctgctaa aagttcaact gctggtgaca ttatttttgc taatctttta ggtgctactt
     6541 ctgctataaa tacagaggtt aatataccaa caggtactcc tatcacaagt gctccaagtg
     6601 ttgcaaatat tgatgctact atcatagatg atattccaaa tttattggca gaaggaaccc
     6661 aatctgcacc agttaaaaag tctataaatg aatatccttc aaatataaat ggtcttaatc
     6721 ctttataaaa tacgaatcct attatcaaaa gtaaacttgc aactgctact aaagcactaa
     6781 gtagaaaaat attttttgct actttttcaa ttacgtattt tgtcttattg ccattattgt
     6841 catttttttc taaacctact aatctagatt ttactatcat aataccctcc cattaaaata
     6901 tacttatcga ttttcgttct gtattcaaaa tttaattttt tataattcgt cttcatcaaa
     6961 tttcctatac ttttattcta ctttttttaa gttaaatcta tattaggatt aagttaagca
     7021 agtgtaaaat tatatttact ttttaacatt aattttactt ttaaaactat atttgacatt
     7081 tactttacaa tatactgttg tttctctata ttaaaatagc agatatagat aataatacag
     7141 tttatgtata atttattacc tatatctgct attaagattt gttaactaaa tttaactttt
     7201 atactacata tataattatt gtatcctcta gtttatatca ctattatttc catcaaattg
     7261 ttacgatact tgatgtttgc aataggtacg aatagccaaa aatataaaaa taggtatttc
     7321 tatcactgag aaaaacagta taatgtgtgg tgtccaaaaa ctcatacctc ctaagtgtaa
     7381 gaaattaaat gatgtcattt gatataataa actaatctta aaaccaattc cccctggtgg
     7441 taataggtat ccaatccaac taacacctat tcccatagga attaagcaaa tagaaaaagc
     7501 aatcagcata gaagtaagag aatccttaca ttttgcagat aaaaataatg ttaaactaac
     7561 cattgcaagt attgaaatta gaccaccaat agctataaga atctgtgctt gtagtaaatt
     7621 tatatttact agactaatta cagaaaacaa catttgaaat gaggttttca aacattcagt
     7681 accaaaagct aaatcagaaa ttgccaaatg gatagaaata catataacaa acattattat
     7741 aaatatagaa aatagagcta atattttagt aaacgctaga cgaatatgtc catgtttcgt
     7801 acaacgaagg atgctatctg attgtgtctg atactcacca gaaaaagtag gtgcagcaat
     7861 tgcaacacat ataattaata atataaaaat ataaaatatt atataatcga aagcatcttc
     7921 tggatttatt aagaaattct taatcttatt atttaactat tttattattt aatatatatt
     7981 aaaggtaaaa aataatatat agatttagat atacaattta tttatctttt caaatataat
     8041 ctataaaata atagttctgt tacgcatttt tttatataat aaaactgact gtaatttaat
     8101 tggcaaagtc tagaagaata ggctttatca attaaattac agtcagctat tttataataa
     8161 tttagtttag atatcgttag tatttcacat ttataacaat tattaatgat attaatatat
     8221 ttattattct ttattaaaat aatatcctgc tcctcttatt gttataattt tctgaggatt
     8281 tttataatct ttctctattt tctttcttaa tccattcact gcaactacca cactatagtt
     8341 atcctctaag tattcattca tccatacatg ttcatatatc tctttagtag tcaaagtctt
     8401 ttcaggattc tctataaaaa agtttaacaa cttattttct gttggagtca attttattat
     8461 tttatcttct aactttatta ctccactagt tctattatat tcctctaatt ttgtattatt
     8521 ttcattatta tttacatatg agttcattct tcttaaaata gcttttactt ttgcgtataa
     8581 tatttctaaa tttagaggct ttattaagta atcgtctcca ccagaattta aagcatttaa
     8641 aatgctttgg tcttcattta tataagtcat ataaacaatt ggacaagtag ttacatttct
     8701 aattttttta caaagagtcc atccatcacc atcacttaga attatttcta aaaatattaa
     8761 atcatattta ttgctaaata tttttccaat agcttcttca caattatatg ctaaatctat
     8821 acttattcct ttttcctcca gaaatccttt tagcttctgg caattatcct tgtctccatc
     8881 tattatcaat atcttagttt gcattataat cactcccaaa tatcattatt cttttatatt
     8941 aaataagcta ttattataaa aaacatagac ttaattttat tgtaaaaaca gttccttttc
     9001 ctagttctga atctacaaag atttctccac catgctctct tataatggat ttgacaatat
     9061 atagccctaa cccttgccct tttggctcat tattgtctct ttctgtaaag aatctgtcaa
     9121 atacctttgg tatattttct ggaggtatac cttttccatt atcagaaatt ttaataattg
     9181 caaatccatc ttctttatcc attgataaaa ttatctttcc atcaaatgga gtaaaggtca
     9241 aagcattata aattaaattc tcaaatgctc ttgtaagcat ctctttattt atctttgcat
     9301 atattttttt ctccaatacg taagcttcaa agtttacacc acatgcttgt gtatctggca
     9361 aattattttc atatatctct ttaataaaat tattcagaca tattttttca ctaataccaa
     9421 tgcttttatc ttgtgatgta aattcttgca aactacgtac tctattttgt acttcatttg
     9481 acttttgatt tatagcataa agataggttc ttgtctcttc atctacatga atattaccaa
     9541 cctttatcaa atctataaat gcttgtatag ccgaaacagg tgcctttaaa tcatgtgcta
     9601 catctgccaa gaatctttta cgttcattta acaatgttgt aagttcatta gttctcactt
     9661 caacttcatg ttcaagattc cttgtaagtt tttcattctc ttttattatt attctattcc
     9721 tgtttatcat cataacagaa aataaaataa caattacaaa cccacaatat tctgtttgcc
     9781 accctgtaaa tataggctca aatctattac tcgtaactgc atcaaataat atgctaattc
     9841 caaaaacagt gtttactgaa actaaaatat aagaacttat atgctcagag catgtctttt
     9901 tatatattga aaccagcgaa gacattaata tatatataaa tacaatatat ttatatatgt
     9961 ctattaactt cccatatatg ttgacaaagt acatatcata tggaaaaaga ataattggta
    10021 tcactataga tatgatactc attgtaattc ctaatggtaa aattaaaaat ctgtacactt
    10081 tattttcttc tattttggat atacttccat ttatacaaat aatacataaa actaccataa
    10141 aatatgacaa atcttcaacc gcatatgtaa agcttaccaa aggcagtcct aacaaatgta
    10201 caaagggata ggatacatgg atggcaaaga aaacacacat acatccaaaa tataagtaaa
    10261 ttttactttt gtttgaaaga aaccatacag acaatgaaaa tatagctatt gcaaaagttg
    10321 tgaagcaaat tatactataa aaaagtaatc gataaaacat catattactt atgtcctttg
    10381 tttctcctaa tgcaggcgga taatacaaac cactataata atgtgaaaaa ttcgatacat
    10441 tgattataat ttctgtattt ttctttgcaa taaaacttac tactgaattt ttaattttgg
    10501 gtctatattt ttctgttcct acatctccta atttagaaac catttctcca tttatccata
    10561 cttcacttgc ggaaaaaatc tcaggaagct ctaaacttag tagcttatcc ttgccttcat
    10621 tttttattct catacgataa gtagcattcc catatggtga ctgagtcccc tttctcatag
    10681 aaaaatctgg atattgacct ataaaaacat attctggttt tatatcttgc tctgtaaaat
    10741 cattcggttt aaatatttta tgattataaa actcccagtc atctatcaaa aagacaagtt
    10801 tacctttagt gaaagtatca ttatctatag ttaaaattcc attacttgct ctaatagact
    10861 tatatgtata tttattatca tatttataca gaagatttaa tcctgttatt gccaacagag
    10921 agactatcaa tacaatgaga gttcctttta gtatattata ctttttcatt gtatcaacca
    10981 catctctttc atagtagtat taaataaaac aagaactatt tattaattac agtatctcac
    11041 ttatggtaca aacttatatc cttttcccca tacggtttga atgtattgat gctccctgct
    11101 taaagaatta atttttttct catctgtgca atatgaacct gcactgtatg ttgatctcca
    11161 atatcaggca ttttccaaac ttggttatat atctctatct tcgaaaatac ttgttctttg
    11221 ttttcagcta gaaacaataa tatttcaaat tccatagtag ttaaatctat actttctcca
    11281 tcaaaagtta cttttcttga atttacattt attactaacg ctgaaaactt taacacatta
    11341 ggaactgatg taatattttt gtactgcctc atacgagcat atattctaag ttcaagttct
    11401 tttaaactat agggttttgt tatataatca tcccccccag ctaaaaatcc ccgaactttt
    11461 tcttcttcat gctcataatt agataaaaat attataggta tgttaacctt ttcttttata
    11521 tcattgcaca ttttatatcc atctatatct ggcaatgaaa tatccaaaat tatacagtct
    11581 aaaactatac tctctactat atccatagca tctttagcag tctctgatgt atatacatta
    11641 aaacctatat tagaaaaata aatttgattt atgtttaaaa tatcaacatc atcatctaca
    11701 accagtaaat tatacattac accact
//
LOCUS       DAKBNN010000124        11544 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000124 DAKBNN010000000
VERSION     DAKBNN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.125"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(50..535)
                     /locus_tag="OUB02_002636"
     CDS             complement(50..535)
                     /locus_tag="OUB02_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8886380.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            787..2010
                     /locus_tag="OUB02_002637"
     CDS             787..2010
                     /locus_tag="OUB02_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCT8886381.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            2014..5148
                     /locus_tag="OUB02_002638"
     CDS             2014..5148
                     /locus_tag="OUB02_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCT8886382.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     AITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            5182..6480
                     /locus_tag="OUB02_002639"
     CDS             5182..6480
                     /locus_tag="OUB02_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCT8886383.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNSGK"
     gene            complement(6756..7001)
                     /locus_tag="OUB02_002640"
     CDS             complement(6756..7001)
                     /locus_tag="OUB02_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8886384.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(7215..9842)
                     /locus_tag="OUB02_002641"
     CDS             complement(7215..9842)
                     /locus_tag="OUB02_002641"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HCT8886385.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(9903..10745)
                     /locus_tag="OUB02_002642"
     CDS             complement(9903..10745)
                     /locus_tag="OUB02_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCT8886386.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(10752..11390)
                     /locus_tag="OUB02_002643"
     CDS             complement(10752..11390)
                     /locus_tag="OUB02_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886387.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         4013 a         1840 c         1688 g         4003 t
ORIGIN      
        1 tttcttatga agttttaact tttttaatag tgacttttac aatcacactc taacctaatt
       61 tctttactat ttctgataac tttaatgtat gttcatctaa taaagttagt tcctcttcag
      121 aaagattaga taattttttt actagagatt ctttgagaat agcttttact gcatcaaata
      181 atttaaatgc tttatctgtt aattcaaccc taagaatcct tctatctttt gggtcaggaa
      241 atttattgac taaaccatgt tccactaatc tgtcaataat cggagtcata tttgatttta
      301 aaattcctaa attagaagct atttgagaaa ttgatgatga atttgatgtc ataagataaa
      361 gaattacctt agcatgtgat ggagccattg gacattcttc aaaataagct ttcattatat
      421 cagagttatt ttgaaacttt tttaacaaat cgctttcttt aaatatatta ttttcaagca
      481 caaataaaaa atctaaaaaa gagtccgcta ttttaagtat attgttgtta tccatatttg
      541 taacatccct tcaatagata atataataaa acattgttgt ttaatatagt ataaattata
      601 aactattaat aaatttaaaa caagaaaaag ttattgactc ataataatta taaatttata
      661 atcaaattag agaattataa attgccaata aagggaagtt ttttaattag tctaaaacta
      721 aacttaaatt ataagacact ttaaattcta caacaagcta gtgtgcaata tataatagga
      781 ggagatatga aaagatattt aatagttttc ttaatgtgta gttcactgtt tttagttgga
      841 tgtggaaaaa cagaagaatc tgaacctgag aaaccaatag cagtttctgt tcaaaaagca
      901 ataggtggag aaattgaaaa cacaaattct ttctcaggaa ctacaaaggt taaagatgaa
      961 acagctgtta ctgcacagac tgttggcaca gttcaagaag tgtatgtaaa acttgggcaa
     1021 aatgtaagaa aaggagatga attattgagt ataagcagtc cagagctaga aaatagtgta
     1081 aagcaaagta aagcctcact agatttagca aaagcaagtt attcaagtgc cactggtggt
     1141 tcattagagg cacaagtaaa tcaggcaaaa acagctcttg acaatgcaaa gattcaatat
     1201 gaggagtctc aaagaaatta tgacaataat aagattcttt atgaacagga agtcattagt
     1261 ttagaccaat ttaaaaaaat agagttttct ttagagcaaa caaaacaaca gttagattct
     1321 gctcaaagag catacgatac agcaacttca aaaagtatac ctcaagctaa agcacttgct
     1381 aaaaaacagc ttgaccaagc tcaagtatca tataatttag caatgagtaa tttagacaaa
     1441 ttaacattaa catctccagt agatggtaca ataacagcta aaaactttga ttcaaaggaa
     1501 atgataacac aatcacaacc agcttttatt atttctaatc ctaatatact agaagttgat
     1561 ttaaatgtag cagaatcaga tataggtaaa tttaagaaag atggaaatgt agatgttata
     1621 attgaagacc aaaggatttt aggaaaaata gattatgtac cttcagtagt tgaccctcag
     1681 acatctttat atcctgttaa agtacttgta aataatacaa acaacaaatt taaggcaggt
     1741 atgtctgctc aagtaaactt aagtgttgaa aaagaaaatg gtgctgttac agtacctaaa
     1801 aaagcaatat ttgaagaaaa tggtaaaaaa tatgtataca tagcaacaaa agacaatata
     1861 gcaaagaaac acttggttca aactggaatt gtaacagagg ataaaataga aattaaaagt
     1921 ggtgtaagtg ataaagatac agtgattatt ggagggataa gtcttatttc tgacggaact
     1981 aagatatttc ctgtagaaaa ggagaagtag actatgaatt taacacagac ttccgttaaa
     2041 cgtcctctca caatcataat ggtcttttta gtattaatag tttttggtgg tattggatat
     2101 aaaaaaatgt caataaatct aatgcctgat atagaaatac cagttgtaat ggtcatgact
     2161 acttggacag gtgctggtcc tcaagacgtt gatgaacaag ttagtcaaaa agttgatgaa
     2221 agtttatctg ccgtttcaaa tgtaaaatct actatatcaa gttcacaaga aagtgtttct
     2281 atggtagttg ctcagtttga atttggaact aatttggatg aaataatgaa tgatgttagg
     2341 tctaaagttg atgcattaca gaccagcctc ccagatgatg cagctaaacc aacagtttta
     2401 aagctggata tgaatgcaca ggctataggt caacttgtca tatctggagg aaatgaaaat
     2461 agttcacaag ccttaagaaa atacgctgaa gatgtaattc agcctaaaat agaatccata
     2521 gatggtgtta cttcagcaga ccttaaaggt ggagaaaaag cacaggttaa tgtaatagct
     2581 gacccagctg ttctttcaaa ttatggagtt agtttatcca ctataaaagg tgtcttatcc
     2641 tctagtaata aaacattccc ttatggtgca attactcaag gtgaggataa aatagtttta
     2701 agagcgattg ataaattaga atccctagat gatataaaac agattcaaat tcctgtaaaa
     2761 ggaggaaata cagttagatt agataatctt tgtagtgtag attatggata tgcagacaaa
     2821 gatagtattt atagatacaa tggaaaagac agtttggtaa tagatatttc taaacaacaa
     2881 gatgccaaca ctgtaaaagt tatgcagagt gcatacaaat acgtagatga attgaataag
     2941 gaaaatcctc aatttaaaat taaaattgct aatgatacaa gttcatacat aagtgaatca
     3001 ataaacagtg taatgagcaa cttgcttata agtgctgtta tcgcatttat tgtaatattt
     3061 gctttcctaa aaagtgtaag agcttcacta gttgttgcag ttgcaatacc aatttctata
     3121 ataggtgcta tagcaattct ctactttact ggtgaaagtt taaatcttat tactgcaagt
     3181 tcattggtaa tttcagttgg tatggttgtt gataatgcta cagttgtaat tgaaaatata
     3241 tttaaatata ggaaaagtgg cagattgagt cttgatgatt gtgctattga gggaacaaga
     3301 actgtaacaa atgcaatact tgcatcaaca ttgaccacaa tagcaatatt cttacctatc
     3361 atgtttacag aaggtatagc taaaataact tttggggcat taggtaaaac actaatagct
     3421 tcactagcat tctcctttat aggtgcaatt acactcgttc ctagtgtatt tgcaaaatta
     3481 agcagaggaa aaaatagtca aaagatgcaa gaaaaagata gtcctatatt tgataaggta
     3541 agtgaagcat ataaaaaatt attaagtgta tgtttgagac atcaacgctt agttgtaata
     3601 tcaagtttac ttatttttgt agcttcctta tttggaatgg gatttatagg tatggacttt
     3661 atgcctgcag cagacaaggg tgaattatct atttctatag aacttccaaa aggtcttagc
     3721 ctagaatcca atgattacta tgtatctatg actgagaaaa aagtctcaga tattccagaa
     3781 attaaaactt taataacaac tttgacatca gattcaaata atagtaaagc aagtatagca
     3841 attgaattag aatctcaaaa aaatagaaaa agaactactg atgaaataga aaaagagata
     3901 attgatagag tggctactgt accagattgt aaaataaatg taagtcaaaa cagtagtgtt
     3961 atgggtggag ctggaggagc agacttcaca ttagaaatga aaggtcctga tttagatgta
     4021 ttaaaaatac tgggaaaaga agctgaaaaa gaatttagct ctataaaagg atttagaaat
     4081 gttgaaactt ctcttacaga tacaacacaa gaagctcaat ttactataga taaacataag
     4141 gctcaagaat atggtgtaaa tactgctgaa attgcaggca tgttacgtac agcaataaat
     4201 ggtgactccg taaccactgc tactattgat gattacaaag tggatgtaaa cctaaaattt
     4261 aaagcagata ctattgatag cttagaagat ataaagcaac taaaagtaaa ctctgcaact
     4321 ggaaaagaag ttcctcttgg tgctttttca gatataaaaa tggcagatgg tctacaatct
     4381 atatctagga ctgatggaga tttttctgta aatataactg ctacacttga caatttagat
     4441 gctggtactg ctacaaaact agcactagaa aaaacgaata aaatggtaat tcctaaagat
     4501 tactctatag gtgttggtgg tgaaacagaa atgatgaatg aatctatggc aagtcttgga
     4561 ttttcaatgg tcatagctat agcattagtt tatatggtaa tggtagctca gtttgaatca
     4621 tttagtaaac catttataat catgttctgt gttccatttg cattcgttgg agttgtgctt
     4681 tcgcttctag ttagtggtca aagtttaagt actgtcggta tgctaggtat aatcttactt
     4741 atcggtattg ttgttaataa tggtatagtt cttattgact acatagagca acttagaaaa
     4801 acaaatcgta acaatgattt aattggtttg gtttcaattg gttgtgcagc aagattaaga
     4861 ccagtcttaa tgactacagc aacaacaatt ttaggtatgt tgccaacagc actatctctt
     4921 ggtgaaggtg gagaaactat gcaaccacta gccatagtta taatgggtgg tctaactgta
     4981 tctacactta taactttggt gcttatacct actatatact taatatttga taaatgggaa
     5041 aataagtttt cagacaaaat gcctaaaata tttggtaaga taacagataa aataggaagt
     5101 ataaaacgta aaataataaa gaaaccaaaa ttgaaagcta aagtttaaat agaatggagg
     5161 gtgattgtat tgaataaaaa aatgagcaaa atagttgcaa ttggctgtgg tgttggtata
     5221 ataactgcca atttaatccc agtttacgca gataataata cttcttctat aaacaaagtt
     5281 gataattcta ttttaagtga ctcaaataaa agaacatctg aaaaggaaat tttgactgct
     5341 agagaagctg ttagtgctgc acttaaaaat agtgaaaaat taaaaatgaa atcagaagaa
     5401 ataaaaatgt taaaagaaaa gttagaagta caagatgagt ttgatagttt tactggaagt
     5461 gacaactcgt ttccatacga ccaaattgga cttcttaaaa atcaaagtga acaggctaaa
     5521 ggctttatgg aggaccaaat agcaaatgat attactaata aatttaacga tttagtttca
     5581 agagaaaatg agctagataa aataaaaaac aatcttgaaa taaaaactaa agaaattaaa
     5641 gatatgaaat taaaaaaaga tttagggtta gtaacatctt tagaaactga atcagctgaa
     5701 cttgaacttc aaacactaca aaatactcaa aaagctaaac ttcaagaact aaaaaacaat
     5761 caagactatt ttaagttatt aactaatata gatttaaata attatcaatt ggataaagaa
     5821 tatagatttg aatcttttag agtaggtggc tcagttgatt catatatgga aggtaaggtt
     5881 aatgaatatt taaaatacga ccaattgatt ttagaacgta cagaagaaag ctttaatgat
     5941 aaagatgaga acaaagcaga tttaccagat agacctaacc ttacaagacc agttgctcct
     6001 acaaaaccag agcaaggtaa cctaactgat gatgaataca aggttcttat ggataagtat
     6061 gaaaaagatt atgatagata taaaaaagaa gttgaagcat acaatcttga aagacagact
     6121 tatgctgctg gtcttactac ttatgcaaat tatcttcaac aaaaatttaa tactgaaaat
     6181 ggtctagtaa cattagaaga cagtaaaaaa gctcttaaaa aagggttaat tgatagctat
     6241 gctcaattac ttgctttaga agatacaatt cagattacta aaaaacaact tgatttatca
     6301 gaaaaacaat taaaaaatac taaattaaga tatgatttag gattaattac tctaactgat
     6361 tataaaaaac aagtggtaag taatgaagat gctaaaaata gctatgactc tcttattgtg
     6421 aattataact cactaaaaaa tggtatagaa aaaccttgga tacttaattc tggtaaatag
     6481 aaagattact ttaacatacg tttgattaaa aaatagttat gcacactcgt ggctttagtc
     6541 atggaagatt cagaaggcaa ataatagaaa tagattagta aaaatatatt tacttataaa
     6601 actgtatact tttatagagg ctatctcaaa atagagataa gcctcttttt tattgtttaa
     6661 attataaaaa tatatttgat tccatacaaa aagagtgcct tatgaactaa aaagttcatt
     6721 ttgagacacc ctcaaaagct taactctaaa tttaattatt ttttatcaaa tgtctcatct
     6781 attttacatc tcaccatgtt aacaaattca gaaatattat atcctctaag tttgcatcca
     6841 catttaggac aataatccag tttcctagat accaaagccc tacaactagg ttctggacat
     6901 cttattttta gactctcatc tgctacttta aatccacatc gtgggcatat actacctgga
     6961 cgtgatgata cttcctttcc acatttagga cactttgtca actttatccc ctccagtaaa
     7021 ctcaatttaa ttgatagttg aaatttctct ctacaataat atatgcaata taattaaata
     7081 cgtttcactt acaactatat aagttaaatt tttataataa aaatccccta tattcaattt
     7141 acaaatactt aatttgatac attgaacaat aggggatttt atttttattt agttacatat
     7201 tttgcagtct attattatct aggatttttt atagatgctt gagctgctgc aagtctagca
     7261 attggcactc taaatggtga acaagataca tagttaagtc ctacactata acagaactct
     7321 actgaagatg gttctcctcc atgctctcca catattccta gttttaattc tggtttaact
     7381 tctctaccta atttagctcc catcttaact aacttaccaa caccattttg gtctagtact
     7441 tggaatgggt ctttttctaa tatttcttca tctacatatt gacctaagaa tttaccagca
     7501 tcatctcttg aatatccaaa tgccatttga gttaagtcat tagtaccaaa actgaagaag
     7561 tctgcttctt gtgctatttc atcagcagtt aaacaagctc taggtatttc tatcatagta
     7621 cctacagtat aaggaacttc tataccttct tcttttatta tagcgtcaac agtttctact
     7681 atcatttttc ttattatttt taactcattt acttctccaa ctaatggaac cattatctct
     7741 ggagaaactt taacacctgc tttgattgct tctatagcac cttgtattat agctttagat
     7801 tgcattacac atatttcagg ataagttatt gcaagtctac aacctctgtg tccaagcatt
     7861 gggttaaact catctaaatc aactattctc ttcttaatat cacttacttt tattcccata
     7921 gattttgaaa gttcttctat agtctcatca tcatgaggca aaaattcatg tagtggtgga
     7981 tctagtaatc ttatattaac tggttttcca tccataactt taaataactc tacaaaatct
     8041 tctctttgca ttggtaatat tttttcaaga gctttttctc tttgttctac tgtattagat
     8101 aatatcattt ctctaactgc tggcaatcta gcttcatcaa agaacatatg ctcagttcta
     8161 catagaccta taccttctgc accaaactct atagctgcac gagcatctct aggattatct
     8221 gcattagttc taaccatcat acatttgtgt ttgtctaccc agttcattaa tttttcaaag
     8281 tttccagtca aagatacttc tgttttctta acatctccta ggtacacatt tcctgtagaa
     8341 ccatctatag aaatgtattc tccttctttt ataactacat catcaagtgc tcttatttct
     8401 ttattaaact catcaacttt tatttctcca catccagcaa cacaacattt acccatacct
     8461 ctagcaacaa ctgctgcatg tgatgtcatt ccacctctag ctgtaaggat tccttctgct
     8521 ttaaccattc cttctatatc ttctggagat gtctctagtc taactaaaac tactttttct
     8581 cctttttcat ttgcttttac tacatcatca gcattgaaat aaactttacc acttgctgca
     8641 cctggtgatg ctggtaaacc ttttgctata accttagctt cttttaatgc cttatcttca
     8701 aattttggat gtaataattg gtctaattga ttaggctcaa ttctcatgat agcttctttt
     8761 tcatcaatta ttccagcttc aactaaatca accgctacat ttattgctgc tgctgctgtt
     8821 ctcttaccat ttctagtttg tagtatatat aatctttcat tttctatagt aaactctata
     8881 tcttgcatat ctttataatg accttcaagt atatgagtaa tttttacaaa ttcatcgaat
     8941 acagctggca ttacatctgc aagagttgat atattttgag gagttcttat acctgcaaca
     9001 acgtcttcac cttgtgcatt cattaaaaat tcaccaaata atttattttc tcctgtagct
     9061 ggatttctag taaatgcaac acctgtacca ctagtttctc ccatattacc aaataccatt
     9121 gattgaatat ttactgctgt tcctaagtta tgagctatat cattcaattt acggtatacg
     9181 attgcacgag gattattcca tgatctaaat acagcctcta ttgcaagcat taattgctct
     9241 tttgggtctt gtgggaaatc attttttatt tcttttttat aaattgcttt aaattctttt
     9301 actatttctt ttaaatcatc agttgtaagt tctgtatcaa ctgtgtatcc ctttgcttct
     9361 tttactctgt ctaatacatt ttcaaattta tattttggaa cttccatagc aacatcagaa
     9421 aacatttgta tgaatcttct gtaactatca tatgcaaatc tttcattttg tgtagcctct
     9481 gctaagccaa caacactatt gtcattaaga cctaggttaa gaattgtatc catcattcct
     9541 ggcatagaga atactgcacc agaacgaact gatactaata atggattctt attgcatcct
     9601 aatgttttat ttaagtcttt ttctaaagta gctaaatgct cttctatttc ttttattatc
     9661 tctgctctta tactttcatt gttaacataa taatcattac atgcttctgt tgttatagtg
     9721 aatccaggag ggacaggtaa gcctatctta gtcatttctg ctaaattggc acctttacct
     9781 cctagtaaag atttcatgtc tttgcttcct tcaccaaaac tataaacata cttagtttcc
     9841 attgttaaat cccccattaa tctttttatt ttaatttata tcatttttat taatagacat
     9901 tattaaattt ctatttcttt gtatatcttt agaccatttt ctttcattat gtctaaaatt
     9961 atacctgctg tttcttctat tgccttattt gaaacgttta ttactgggca tccaattctt
    10021 ttcattatct cttctgaata atctaactcc tgaagtattc tttctaaatt agcgtagttt
    10081 gctttactgg acaatcctaa agcttttaat ctttgggttc ttatctcatt cagcttttca
    10141 ggtgaattag taagacctat tatcttttta gtatctatct caaacacttc tttaggaatt
    10201 ggtatttctg gaactaatgg aacattagct acttttatat ttttattagc taaatacata
    10261 ctcaaaggag ttttagatgt tcttgatatt cccactaaaa ttatgtctgc ttgaagaaca
    10321 cctctagggt cttttccatc atcatatttc actgcaaatt ctatagcttc aactctctta
    10381 aagtaagatt catctaattt tctaattata ccagcttctc ttttaggttt tcttcctgtt
    10441 ctcttgctta tttctcttag tatatctgtc attaaatcta tacaactaag accttctctt
    10501 tcgcaatatc tttcaacaat actgcataat tcttcatcaa ctaatgtata tacaattaaa
    10561 gcgttttcag cttttgcatt ctccaaagtt tcaagtagta ctcctttatt aatcatgtat
    10621 ggaaatcttt ttatttcata agtttcattc acataaaact gagacataca tgcctttgca
    10681 acttgttgtg cagtctcccc tactgaatct gatactgcat atataattag gtttttcacc
    10741 agcatacctc cttaattgtc aactatttct aagaatagct tagttatgtt ggtttttgat
    10801 attctcccaa caactctcat atgatttttg tcttctccct tatattcaac aatcggaatc
    10861 gaatctactt catgttctat tatttttctt gaagctaata aaacacttgc tcctttagtt
    10921 aaagtgacta catttggagt tcttgtcatt atcataccta ttggcatttt atttatatca
    10981 gaacctccta tagttgcttt tagcaagtct tttctggaaa caactccaca taactcttca
    11041 ttctcatcta taataactat actccctaca tcagataaaa acatagtgac tattacatca
    11101 tatatacttt catccttctt agctagtaca ggcatgctca ttatatcctc tactgttttt
    11161 tcttttacac ttttaccaat taaatttatt tcactaactc ctgaataaaa atatccaact
    11221 tttggtcttg cgtctaatat tcctgtcatt gttaatatag ctaaatcaga tctaagtgta
    11281 gcacgagtca cattcaagct ggaggcaata ctttcactcg ttattggttc attttcctta
    11341 actatatcta taatttttag ttgtctttca ttaagctgaa taataatcac cttctttcat
    11401 tgtgctatac tttttatctt ttcgcatctt atatagtata ttatataact atatatacca
    11461 ttctgtaaac agatttttta ttgaatacga tatatttttt actaagttct ctcgcttaaa
    11521 gatatttatt atatattaat gggg
//
LOCUS       DAKBNN010000125        11398 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000125 DAKBNN010000000
VERSION     DAKBNN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.126"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            65..949
                     /locus_tag="OUB02_002644"
     CDS             65..949
                     /locus_tag="OUB02_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCT8886388.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1048..2223
                     /locus_tag="OUB02_002645"
     CDS             1048..2223
                     /locus_tag="OUB02_002645"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HCT8886389.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2351..3205
                     /locus_tag="OUB02_002646"
     CDS             2351..3205
                     /locus_tag="OUB02_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886390.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFYEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3249..4484
                     /locus_tag="OUB02_002647"
     CDS             3249..4484
                     /locus_tag="OUB02_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCT8886391.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4585..5526
                     /locus_tag="OUB02_002648"
     CDS             4585..5526
                     /locus_tag="OUB02_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HCT8886392.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5745..6098)
                     /locus_tag="OUB02_002649"
     CDS             complement(5745..6098)
                     /locus_tag="OUB02_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT8886393.1"
                     /translation="MDRKRELKQLYKEMKFDIGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFIFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            6394..7305
                     /locus_tag="OUB02_002650"
     CDS             6394..7305
                     /locus_tag="OUB02_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HCT8886394.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7566..8000
                     /locus_tag="OUB02_002651"
     CDS             7566..8000
                     /locus_tag="OUB02_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886395.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8023..9558
                     /locus_tag="OUB02_002652"
     CDS             8023..9558
                     /locus_tag="OUB02_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886396.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVVLVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDEVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9642..11009
                     /locus_tag="OUB02_002653"
     CDS             9642..11009
                     /locus_tag="OUB02_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886397.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11044..11268
                     /locus_tag="OUB02_002654"
     CDS             11044..11268
                     /locus_tag="OUB02_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCT8886398.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4537 a         1060 c         2110 g         3691 t
ORIGIN      
        1 acaatttata taaactaata aaaattaaat aatattaatc aacttattga atggaggaat
       61 aaaaatgaaa tttgtgactt tttgtagtag caatgaagaa aaaataggtg tatttaattc
      121 agaaacaaat agtatatatg aaattaatag tttaggatta agtaaattat atactgatat
      181 gaatgatttt attgaaaacg tatctacagg agatttagaa aaaataaaaa acaattcatt
      241 tgaaaacgca aagtgttata agttagaaga agttaaatta tgttccccaa ttgtaagacc
      301 taaaaaagat attatatgtt taggtctaaa ctataaagac catgtcaatg aaatcccaga
      361 tggagttata aaaaacgtag ttatgccaga ttatccaatt tatttttcta agagagcaga
      421 caaaataatt ggagtggatg ataagataag cttacatgga gatttggtag agaagttgga
      481 ctatgaatca gaacttgcag taatcatagg taaagaaggt ataaatatat caaaagaaga
      541 tgcttatgaa tatatatttg gatatacaat agtaaatgat ataagtgaaa gagcacttca
      601 agataaacat gttcaatggt ttagaggaaa aagcttagac acacatacat ctatgggacc
      661 ttatatagta cataaagaag aatttgaaca tcctttaaaa ttagatataa gtagtgtagt
      721 aaatggagaa gtaagacaaa attctaatac agagtacttt atatttgata tacctactat
      781 aataaatgat ttatcaagag gaatgactct aaagccagga gatataattt caactggaac
      841 tccagcaggg gttgctatgg ggatgaatcc tcaagtatat ttaaagcatg gcgatatagt
      901 tgaatgtaaa gtcgaaggaa taggagtttt aaaaaatata gttgattaat ggataaatat
      961 gccatataat tttattagta cgaataattt aattaatata ggattaatca aacttatgat
     1021 taatttatta tagagaggga tgagtatatg gcaagagaac tagaattaaa gtatggctgt
     1081 aatccaaatc aaaaaccatc aaagatttat atgaagaatg gagaacttcc tatagaagta
     1141 ttaaatggaa aacctggata tataaatttt ttagatgcat ttaatagttg gcaacttgtg
     1201 aaggaattaa aagaagctac aggtctccca gcagcaactt catttaaaca tgtaagtcca
     1261 gcaggagcag cagtaggggt acctttatca gatactttaa agcaaatata ttttgttgat
     1321 gatttagagc tttctccatt agcttgtgct tatgcaatgg caagaggagc agatagaatg
     1381 tcttcttatg gcgattttat agcactttca gatgtatgtg ataaggagac tgcaactatt
     1441 atagcaagag aggtttctga tggtataata gctccaggat atacagatga agcccttgaa
     1501 atattaaaag gaaagcgtaa aggcaactat aatattgtaa aaatagatga aaattatact
     1561 cctgaaccta ttgaaacaaa ggatgtttat ggaattactt ttgaacaagg tagaaatgaa
     1621 atattaataa atgaagacct attaaaagat attccaactg ataataaaat ttttacagat
     1681 agtgcaaaac gtgatttaat tatagctctt attacattaa aatatacaca atcaaattct
     1741 gtttgttatg ctaaagatgg acaagtaata ggggtaggtg caggtcaaca atcacgtatt
     1801 cattgtacta gacttgcagg taacaaagct gatacttggt atcttcgtca acatcctaaa
     1861 gtattaaact taaaatttaa aaaggatata ggtcgtccag atagagataa cactattgat
     1921 gtatatcttt ctgatgatta tatggatgtg cttgctgatg gcatatggca aaactttttt
     1981 gaagaaaaac cagagcctct aactggagaa ggaaaaagag catggcttaa aactttaaca
     2041 ggagtagcac ttggttcaga tgcatttttc ccatttggag ataatataga acgtgcaaaa
     2101 agaagtgggg taagttttat agcacaacca ggtggttcta ttcgtgatga taatgttata
     2161 ttaacttgta ataagtataa catagtcatg gcatttacta aaaatagatt attccatcat
     2221 taaaatactg aaagtataaa gataatatag attaagtaaa aatctacaac aagttttgtt
     2281 gtagattttt ttatttgctg tataatttta aatagaactt tatttttaga tattcaattt
     2341 ggaggttgat atggctttaa atataagaaa ctatagccta ttatgtaatc aaggaacagc
     2401 taaatttaat gaagatgtag tcggtataaa tccatttggc gcatgggttt tagatggtgc
     2461 tacaggactt aatggaaaaa atttagtgtc aactgaaagt gacgcaaggt ggtatgttca
     2521 gtggtggaat aggtatttat ataaaaatat aaataaagaa gaatcattaa agagtattat
     2581 taatactgga ataatagaag taaaaaaaga gtatgaacta agattaaatg ggtttaaggt
     2641 tgaaaaatta gatttaccat ctagttctat agttgttgta aaatttcatg aaaataaagt
     2701 agagtatttt ttattaggag attgtgcttt gtttattaaa aatggagatt ccaaagttat
     2761 aaaagatagg agtatttgta aatttgataa tactgtattt tatgaaatga gtaaactttc
     2821 taacttggaa gagatggcat ttgatgagat tagaactagt gttatggata caatagtaag
     2881 caatagattg aagaaaaata caaaagaggg ctattggata ttggattttg atgaggaagc
     2941 tgtggaaaat tctatatatg gatacattga aataaaaaaa gattttcaat tgatgctaac
     3001 gagtgatggt tttacaagtg catgtgatag atataatcta ataaaagaag aagaattgat
     3061 acgtatagca gaaaaatttg gtataggata tatacataat aaagtaaggg attttgaaga
     3121 taatgactat aaagcggtta aagtgccaag atttaaagta aaagatgatt catcttgttt
     3181 atatttagat atatataatg attagactgt ataaaactat ttaattttag aaaggaggca
     3241 agtgaaaagt gaaaattctt catataataa cacaaaaacc aaatagtaca ggtagtggta
     3301 tatatttgtc tggtatgata aaagggttcg aaaaaatcgg tcataagcaa gctgtaattg
     3361 caggtataga tgtaaatgat gatgtaaatt gttttccaag tgaagtaagt ttttatccag
     3421 taaagtataa ttgtggggag ttaaattttc ctgttgtagg tatgagtgat tctatgccat
     3481 atgaaagtac aagatataaa gatttaaata tagacatgat aaatagattg aaatatcaat
     3541 ttaaagtaaa tatagataag gctatgaatg attttaagcc agaacttata atatgtcatc
     3601 atttgtacct tttaactgca tttgttaggg aaatggtaaa agatataaag gttatgtcta
     3661 tttgtcatgg aacttgttta agacagttaa ataccattga tttagagaaa gaatacataa
     3721 tagcaaatat aaggaaatta gatttaatat ttgctcttca tgaaaatcag aaatatgata
     3781 ttataaagac ttttggagta agtgaaagta aagtagttgt aattggtagt ggttataatg
     3841 atgatatatt ttataataaa aattataaaa taaaggatga taaaataaaa atagtatttg
     3901 caggtaagat atgtaaatca aagggactga ttccatttat aaaagctata tctaaattaa
     3961 aatatagtaa agatttaata gaagtcaatt ttgcaggtac tggaagcgat atcgaatcat
     4021 ataatgagat agttaaactg gcaagtaagt ctccatttaa aatgaatttc ctaggaaagt
     4081 tagaacagag agatttagct gagctattca acaggtcaca aatatttgtg ttaccatcat
     4141 tttatgaggg attaccagtt gtagttttag aagctttatc ttgtggcaca gatgtaatca
     4201 ctactgatat tcttggtgtt aaagagtgga ttggtagtga gattaataat tctggaaaga
     4261 tagaatatgt ttctctacct tttatggaga aagaaggtat tccaaaagat gaagagttat
     4321 atgattttga aaataatttg tataatgcaa tagattctaa gatacaatct ttattgaata
     4381 actcaaataa gaaaacatct gtagatatgt caaaaaaaac gtgggatggc ttagcatata
     4441 gaataaatga agttatttta atggatgaat tgtgtttagt ctaaaattat ttaaattata
     4501 aaaaagtgta actattttta cactattttt aaatgatttt atattatact aatactaaat
     4561 atatttgttt tagggaggta atttatgagg attagtaaaa aggtattggc gttaggtatt
     4621 tcagcattac tgttaagtgt tagttttcca acatcaataa acgcactaga taagattgaa
     4681 aatattcaag gagtagataa atatgaaaca gctgggctta tttcagataa gcaagatttt
     4741 acaacagcag ttcttatcaa tgcagactct acaatggtag atgggattgc tgcaagtggt
     4801 cttgcaggag taaataatgc agctatttta ttaactaata aggatgatat accagaagca
     4861 acgcttcaaa gactgaatag agttactaag atatatgtaa taggtggaga aaattctata
     4921 agtaaagatg tagaaaaaat gttacttatg agaagaatgc aagttataag aattgatggt
     4981 gtagatagag tagatacaag ttataaaatt gcaggcgaaa tagaaaaaat taaaaacagt
     5041 gataagatgt ttttagtaaa tggatttaag gatgaagctg atgcagtaag tgttgcatct
     5101 gttgcttata gagatggagc tcctataata ttgacaaaag atataccttc agcagaagaa
     5161 gacacagact tagacccatg gtttggagtt tctcctgttc cagtatatgc tattggagga
     5221 gagtctacat taagtgatta catagtgagc agatatagag caactagaat tggcggtgtt
     5281 gatagatatc aaactaataa aaatgttatt gaaaagttct ataatggagc aaaagagttc
     5341 tatataacaa gtggagacga tttagtttat gctttagttg catcaccact tgcaaaaaat
     5401 gctcctgttg ttttagtttc aaataaaagt gataagagta tattaagtgg agctagtaaa
     5461 gtaacagcaa taggtataag tgataagagt attatagagc aatgtttgga tgctgtaaag
     5521 aagtaatgga gtgttattag taaaatgaat atacacattg aaaattagta gattgttcat
     5581 tttataatgg tataaaatat atgggaagag gctatcaaat ggagataagc ctctttttca
     5641 ttgttaaaat tatgaaaaga tactttattt tatataaaaa agtatcttat gaactaaatg
     5701 tctattttga gacactatct acttttctta tattatatta ttttttatat ggaataaatt
     5761 tcataagtat tatcttcctt taatttttct aaccaaacca tctctaaaat atctagttct
     5821 tctaaataat ctttctctgt tttgttatca tcatgtttta taagttctaa aacttcaaat
     5881 ataaatgctt tttcaccata ttttttccat tcattattta attcttttat catacatgag
     5941 ccagcaccaa gctgaaattt gagagagtta aatttgcttt ttatgtcatt actttttcca
     6001 aggtaaatct ttttagtaat atcatttttt attatgaata cacctatgtc aaatttcatt
     6061 tctttgtata attgttttaa ttcacgtttt ctatccatga ttttagatac ctctctttat
     6121 aattgatatg taatatgtaa gtgtaaaaat atattacata taaattataa agagattaat
     6181 tttatatgta aatagttttt ataaatttaa ttatgaatta gtcatcaata aacaagaaat
     6241 ttagctgtag aaatccatta taaaggctat tataaatttt aatatgctaa aaatataaaa
     6301 agaatagttg acagtgtaga aaatatggaa taaactaaaa gtgtaatagt aatatgaaat
     6361 tatatattac attgaaaggt gtgaatgatt attatgaata aaaaaataga attaataggg
     6421 aactttgcaa gtgattggat taggtatagt gattatgaat ataaaattac tgaagaagat
     6481 gaattatata tagttccaac agaagatgct gtgtttacca tgtataaccc atttgatgta
     6541 gcagatgata taatagtaga tataattcgc ataggacaag aggcattaca agatatatca
     6601 aaagagacaa aagaaatgaa actaaagaaa gaagtacttg aatttgtaaa aaagtatgga
     6661 ttattgggtt taatatattc aagtgtatat aatagagata tagttggaga agaaagagta
     6721 ctgatgattg aaaagaatta tattacaaaa gagaatatat tgagtacaga tgaatatgta
     6781 aacatgttta taccatttgc aagtgatgaa gatgtgtatt ttaaaaagta tagaaggggt
     6841 atagatgtat caaagagaga agaatctcca aagttttttg gaaagagacc attgatttta
     6901 gatttagtat tttcaaagtt ctacactgaa caactagatt ggattgtaag ttttgctaaa
     6961 gatatgtgta agcattttaa tcaaatttgt gtatataaaa atacatctaa tctattaact
     7021 gaaagtgtta ctattatgtc tggagggttt aatcctcaaa agataggatt ttctataagc
     7081 caattagata aaccaattat tgcatgggag tttgattctt tgaaaacagc tattgagact
     7141 atctatgcct ttgcaattac aagtgaacct tcttctttaa ataaatgcaa gtactgtggt
     7201 aaaatttata ttcctacaaa ttctaaatct caatactgtt caccttcttg tagaaattgc
     7261 tcaaatgtaa agaaaagtag aagtagagcc aaaaaagata aataaaatta ataaatatat
     7321 cattgtttag attttagtaa ataactactg aattaaaagt tgttatagaa aattatatat
     7381 ctaaatgatg gtattttttt tgttgagaaa attgaaaagt ttaattgaga tagtaatttt
     7441 gagttgacaa aaaccacttt taacactact attataagtg gttggcaaaa taatatatac
     7501 taatttaagt atagcgtaaa aaacaaaaaa agtcaatgga aaaaataaaa aaggagaatt
     7561 taaaaatgat atcacctatt attttgagtt ctataaatca gaatttaaag gaaatagaaa
     7621 gaaatgaact gcttgaaact aatatagaat ctggagatta tggtctagct ctttctgaat
     7681 ctgatgtaaa agatataata aattcgagag ataatacttt aaaggggtat ggaagaatag
     7741 aattagatat aaaagttaca aaacagttaa tagaaaatat atatacatca caatatacaa
     7801 atgtggacaa ttatttagag gctattaatg atatgcaaga aattttttat tatttaaaaa
     7861 atgaaactga tgacaagata tgtgatgatg agattataga aatactgggt gaattttatg
     7921 aaaaattttc aggcaatatg gacaatgtaa gaggagaagc agacgaattt gcaaagaaat
     7981 ttaaatttgg agaagtttaa tatatgataa gagagatttt gtatgttaaa taatttaagt
     8041 aatattaata taaacgaaaa taatttaata aagaaccaat attccatatc tctacttaag
     8101 gaatgtctaa attgtaaagt gatagatgaa agagaagtat ataatataca acaagaaata
     8161 tctctaatac ttatggattt gattaaaaaa tatacaaatg gtcaaagcac atcagtaaaa
     8221 acagaagttg ccgaaaaact gttgatttca atatggtatg ctattgatgc atatataaat
     8281 aagcttggag atatagaaaa aagagttgtg gagttgactg ctaagaatgt taaaaaggtg
     8341 taccttggag gcattgaaat attagaggaa gagttagtta ggctaaaaga tttttataaa
     8401 ctaatgatga gtagaaaatt ggatactaat tcaatagcat ataatgatac cttgatggag
     8461 ttatcaagtt tctttaaaag ttatattgta aaatttgaag ctcaggacgt tccagtaagt
     8521 atagattatc cattggcact agatgatatg gatgttaagg gtatttatta tgtgaagaat
     8581 tatattgaga gtatagatat agaaaataga ttttgtaacc tatttaagaa aaaagataga
     8641 gaaaaattat tttatgacta tggggtgact tataaaatag attgtgatgt tgtacttgta
     8701 aatttatttg aacttattat aaataattgt attttttcta ctattttagg aaatgaagtg
     8761 actaatttag aaatttcaaa atatgagtat gaatttttag aaaaacaatt taaaaatatg
     8821 tacaataata ttactgaaga tgaagttata ggctctgaag aaagtaatat aattgaattg
     8881 attgaagtag atgatagttt taacactgaa gtagatagta atttggatta taatgatttt
     8941 gaagaaatta acagagaaat tagaataaat aaaaaaataa ctttactact tatggaagca
     9001 attgaagtat taataaaaag attaagcata gaagatgaag atgtaataag atatattaaa
     9061 aaatatgaaa agatatttat tgaatctgtg ctaagctcaa taaaaagtaa cacttttaaa
     9121 agtatggtaa ctgtgagtaa aaataaaaag gaacacttag aagattatat tgttgatgat
     9181 gaaagtaaat tggatgatga atcgtttaga gagatattca atgagatatt agatagtaca
     9241 tctataattg aaaaaataag aataataaag gaaaatatca atgctaaaaa agactttatc
     9301 gatattcttg aatctgaatg tttatttgga gaagagtatt taatgttgtt tgcatcatta
     9361 agtgaattag aattggcaat attgggtagt gtagtatttt atgaagatat tagaatgaga
     9421 gaaattaata tattagaatt tatgttaaat gagaaaactg aaactacttt atggaagata
     9481 gagtacatta aatttatgaa gaggcaaagt gaagataaga tatactccat agaagagcat
     9541 atgaataaaa ttaattaaca aaaaattgtg aataaataga atgttagtat ataatataat
     9601 ctgtaaagca tatcaaaaat acatatattt ggaggaaaat tatgaaagag tatatcataa
     9661 ataatgttga agaaataaga gaagaattaa ttgatttatc taagaaaata tgggaaaatc
     9721 cagaattagc ctttgaagaa aagtatgcaa gtagcattca aaaagaatac ctaaagtcaa
     9781 aaggatttaa aattgaagaa gtagaaaatt tacctactgg atttatagca agttttggag
     9841 agggtaaacc tgtgattggc atacttggag aatatgatgc tttaccagag ctatcacaat
     9901 gtgtaagtgc agaaagaaag ccattggtag aaggaaaagc tggacatgga tgtgggcata
     9961 acctgttggg tgtagcaggt gtaggagcag ttgtttctat aaaaaaatta atagaagaaa
    10021 ataaatttaa tggaacaata aaatattttg gttgtcctgc tgaagaagaa ggtggaggaa
    10081 agacagttat gtgtataaat ggctgttttg atgatgtaga ttgtgcattt acatggcatc
    10141 cttttgatat aaatgcacct tggagaggtg gaagtctggc aaacttatct gtaaaattta
    10201 aatttaaagg gataacagcc catgcagctc aagctcctca taatggtaga agcgcacttg
    10261 atgctgttga aattatgaat gttggagcta attatttgag agaacatgtt attgactcta
    10321 taagaatgca ctatgttata actaatggtg gtggaagacc taatgtagtt cctggatttg
    10381 ctgaaagttg gtactttatt agaggtaaaa aagcaaagga tgcagaacac gttcttgata
    10441 gattaataaa agtagctcaa ggagcagcaa tgatgacaga aactgaaatg gagtataagg
    10501 ttacagatgg aatatatgac tatataccaa accaatgttt gactgattta gtttatgaca
    10561 atatggtttt tgttggttgc cctaaaaata ctcctgaaga agaagaattt gcaaaaaagt
    10621 tgtgtgatac tcttactaga gaagaaagat taggtgtagc aacatcatta caaaatgata
    10681 aatctattgt agaaagctat atacacaagg aaatagttga cggtgacaaa gataaaggac
    10741 tagctggttc tactgatgtt ggagatgtaa gttatgtaat accagtggct caatttgcaa
    10801 tggcagcttg gcctgttgga attgcaagtc atacatggca gtcatgttca tcagcaggtt
    10861 caaatatagg attttctgct atgataaatt cagctaaagt actggcttgt agtgcttatg
    10921 atgtatttat ggatactaaa attattgatg aagcaaaaat tgaatttgat aaatcattag
    10981 atggacaaaa atttaaacca ttggtataat tttggtaata aaatatttct ggggggaata
    11041 gctatgagag aaaaagttga aaaagtgctt gaggaaaaga taaaaccagt attacaaaga
    11101 gatggtggag atgtagagct tatagatgta aatgaaaatg gtgtggtttt agttagacta
    11161 caaggggcat gtagtgggtg tccaggagcg actatgacaa tcaaagctat tattgaaaat
    11221 gttttggtga gtgaagttcc aggagtaact caagtattag gtgtttaata gttagcgttt
    11281 attaaatgta tgttatacat aagtatttaa taaagaagta aaaaaaatag tgaagcaata
    11341 tatatgaaaa agtatgagaa agataataaa acattataga aataatgaaa tatttcat
//
LOCUS       DAKBNN010000126        11372 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000126 DAKBNN010000000
VERSION     DAKBNN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.127"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1074
                     /locus_tag="OUB02_002655"
     CDS             <1..1074
                     /locus_tag="OUB02_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCT8886399.1"
                     /translation="IGVGWSLMALDANRVNKIISKPWTSDGLNFSERIWGKHRPALIN
                     ELHTKLTQSIIRGENPKVLVNEFSKKFNVSKSQAKNLIMTESAFFASASRKDCFNDLD
                     VEKYEIIATLDLKTSNICRELDGKVFDMKDYQVGVTAPPFHCRCRTTTAPFFSDEEGY
                     RAARGEDGKTYYVPSSMKYNEWYEKHIEGRLGKEKANTLRKMQLNESKDRKQFEEYKK
                     VLGNEIPSKFDEYQHMKYNDTIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKP
                     NSVFDNYKDGKLIQRRYYGNTGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKV
                     KREKDMELTKAHKIANKDILKGE"
     gene            1076..1324
                     /locus_tag="OUB02_002656"
     CDS             1076..1324
                     /locus_tag="OUB02_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886400.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            1392..1955
                     /locus_tag="OUB02_002657"
     CDS             1392..1955
                     /locus_tag="OUB02_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HCT8886401.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYANI
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            1967..2866
                     /locus_tag="OUB02_002658"
     CDS             1967..2866
                     /locus_tag="OUB02_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HCT8886402.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYVTPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWESKTLTNQRKWSTLVHPADIDQTNYVASI
                     GNITQVYNEEQKFPEMDAYCISKIYADWIALGKTAETTALTTANILEVFDSLMEKMTE
                     ARVPAVGRILYVTPGVDTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDSVKIVKVPSNL
                     MKTVYDFTTGWKAGAGAKQIFMSLIHPSAIITPVSYQFSTLDEPRAVTEGKYLYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            2866..3099
                     /locus_tag="OUB02_002659"
     CDS             2866..3099
                     /locus_tag="OUB02_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886403.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKALKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            3109..3510
                     /locus_tag="OUB02_002660"
     CDS             3109..3510
                     /locus_tag="OUB02_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886404.1"
                     /translation="MENNRIDDIERRLQGFGYVLKNGDKWLIDFVREKIENIIKLDCN
                     IGEIPKELHNIEVDMIVGEFLFTKKNMGQLDIESLNFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDTLVNYLLTYGKNKILTFRCLRW"
     gene            3504..3866
                     /locus_tag="OUB02_002661"
     CDS             3504..3866
                     /locus_tag="OUB02_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886405.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDISVKAGSKLIITNQNNITKEYIRSGES
                     AIYPNHQEIVLELLEDKA"
     gene            3866..4291
                     /locus_tag="OUB02_002662"
     CDS             3866..4291
                     /locus_tag="OUB02_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCT8886406.1"
                     /translation="MGRGGSVNFKELKKLQRNLQRLEESQLDKFCRDCAKELAARLIG
                     KAIKRTPVDTGVLRQGWNGVAYARALPVIKAGDTYIIEIINPIKYASFIEYGHRTRNH
                     KGWVKGRYMMTISVQELRGQVDVIIEKKLINLLKKVFDA"
     gene            4284..4721
                     /locus_tag="OUB02_002663"
     CDS             4284..4721
                     /locus_tag="OUB02_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886407.1"
                     /translation="MLNNIIDGISVKLDKSFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNNTDKMDTVELFGGLKRGDKLE"
     gene            4714..4890
                     /locus_tag="OUB02_002664"
     CDS             4714..4890
                     /locus_tag="OUB02_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886408.1"
                     /translation="MSKTLSKEENYKFTKEQIVNSKRYVNRKDLLNAILKEDELYSLS
                     EVEDMINNFIKGVS"
     gene            4891..6201
                     /locus_tag="OUB02_002665"
     CDS             4891..6201
                     /locus_tag="OUB02_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCT8886409.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHDKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIAIAKYSGIRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYV
                     IWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDTELLESSLIYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEIHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTEDVLVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            6221..6697
                     /locus_tag="OUB02_002666"
     CDS             6221..6697
                     /locus_tag="OUB02_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCT8886410.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            6750..7007
                     /locus_tag="OUB02_002667"
     CDS             6750..7007
                     /locus_tag="OUB02_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886411.1"
                     /translation="MIKIKDKYEIKDSISFDYSKKRHIEECVSEIYKKAGKYLIDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            7009..7449
                     /locus_tag="OUB02_002668"
     CDS             7009..7449
                     /locus_tag="OUB02_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8886412.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFVGEDGKPVEWELKAIDSD
                     RDRQLRKNSAIRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            7449..7643
                     /locus_tag="OUB02_002669"
     CDS             7449..7643
                     /locus_tag="OUB02_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886413.1"
                     /translation="MEGGDYDASVAHYCLHKFKWKPHEYTDLPDYERAFVAASIDIKV
                     EEEIKEEKKTAKEARRSRRR"
     gene            7772..7930
                     /locus_tag="OUB02_002670"
     CDS             7772..7930
                     /locus_tag="OUB02_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886414.1"
                     /translation="MDNFLQGVLASLVASLIVYLTSKLFKKVKSHSNTTKSDFDFKLT
                     IKFKRNKH"
     gene            8386..8556
                     /locus_tag="OUB02_002671"
     CDS             8386..8556
                     /locus_tag="OUB02_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCT8886415.1"
                     /translation="MSPKKIGRPVVGSPKTNDIKVRVDDETNEKLDEYCKKNNLTKAE
                     AIRQGIHLLLEK"
     gene            8683..9525
                     /locus_tag="OUB02_002672"
     CDS             8683..9525
                     /locus_tag="OUB02_002672"
                     /inference="COORDINATES: protein motif:HMM:NF014548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HCT8886416.1"
                     /translation="MKTNLELIKSENFGEVECNFYENDTKDILMTREQIGMALEYTEP
                     RIAISKIHERNKDRLDKFSTVVKLVTVEGNREVLRDTIVYNTKGVMEICRFSRQPKAD
                     TFMDWVWDVVESIFKTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDDAGF
                     DNKSKLLTAKTLYKKAGIDLPIEIDEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEII
                     KKIKLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIQGEKKNYHVV
                     YKIE"
     gene            9580..9756
                     /locus_tag="OUB02_002673"
     CDS             9580..9756
                     /locus_tag="OUB02_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886417.1"
                     /translation="MCKSLLDDYNLKTDKERKDFIKFAILLYKLEQDNKEKFYEYTEI
                     LRGILREQQERENK"
     gene            10124..10684
                     /locus_tag="OUB02_002674"
     CDS             10124..10684
                     /locus_tag="OUB02_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886418.1"
                     /translation="MKKKVCFLFSILIVICLAIVGCSNSESPEESSKNNEPKKEEKKD
                     KEVVIGEKIISDKMEITINNIEFSYDVLPKVKESLYTHYPAESGKVYIDIAADIKNTQ
                     KQELNCSDLLTIEANYNDGYKYSSQTIVEDETTGFTYDNITSIDPLETKGVRFIIDCP
                     DEVKTSDKPLVLSFTFDGNKYVYKMK"
     gene            10727..11296
                     /locus_tag="OUB02_002675"
     CDS             10727..11296
                     /locus_tag="OUB02_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8886419.1"
                     /translation="MNDTVEYIFCSECGSRCLKGSKFCNECGTKIDDVKIQKESITKD
                     KEYDLKGVDLQHIMQETSFIKASSVRRLKELTGIELDDCRKILEEPYQKYYNENADML
                     EEKRKKEDDISKIRKAKEEKKEKEKIACCPACGSTSLTAHKKGFGIGKAITGATIAGG
                     IGLVAGNIGAKKVRVTCLSCGKQFWAGKK"
BASE COUNT         4701 a         1149 c         2158 g         3364 t
ORIGIN      
        1 attggtgttg gctggagctt aatggcttta gatgcgaata gagtaaataa gattatctca
       61 aaaccttgga ctagtgatgg gttaaatttt agcgaaagaa tttggggtaa gcatagacct
      121 gctttaatca atgagttaca tactaagcta actcaatcaa ttattagagg tgaaaatcca
      181 aaagttttgg taaatgaatt ttctaagaag tttaatgtat ctaaatcaca agctaagaat
      241 ttaataatga ctgaatcagc tttctttgca tcagcttcaa gaaaagattg ttttaatgat
      301 ttagatgtag aaaagtatga gattattgcc acactagatt taaaaacatc aaatatatgc
      361 agagagttag atggaaaagt atttgatatg aaagattatc aagttggagt tacagctcca
      421 ccatttcatt gtcgttgtag gacaacaaca gctccttttt ttagtgatga agaaggctat
      481 agagcagcaa gaggagaaga tggaaaaaca tattatgtac catctagtat gaagtataat
      541 gagtggtatg agaaacatat agagggtaga cttggaaaag aaaaagctaa cacccttaga
      601 aaaatgcaac taaatgagag taaagataga aaacaatttg aagaatataa aaaggtatta
      661 ggaaatgaaa taccttctaa atttgatgaa tatcaacata tgaagtataa tgatactata
      721 aggtatgagg aagctaaaaa gctttataag gatgtaaatt ggcaagttaa gaatcagaga
      781 aatttaacta gtggaagtgt tcattcagtg ccatttgaat caaaaccaaa tagtgtattt
      841 gataattaca aagatggtaa attaattcaa agaagatact atggtaatac aggaaaacct
      901 agattagatt tagacttaac tgaccataga aacccaaagc aacataaaat tgtacctcat
      961 aaacatgatt ggttagctga tgaaaataac catgataagg taaaaagaga gaaggatatg
     1021 gagcttacaa aagctcataa aatagctaac aaagatattt tgaaaggaga ataaaatgga
     1081 taataaattt aagaatttac aacatttaat tgattctatt gatttaggat tagatataga
     1141 atttgactta tataataaac catataatat atctattgga gatgatgata caaggtttat
     1201 tactctttgc cctaatggag atacaaagta ttataaaaat ggtaaggata tggttgataa
     1261 ttacaacatt gatggtaaat tactaaagga tttatggaaa gatatacaaa tagtaaatat
     1321 gtaaaagcac ttgctaaata ataaattagt aggtgctttt attttgtaaa aaatgaaagg
     1381 agaaatttaa aatggattgg ttaaaagaat tgctagaagg aataaaagta gaggataaca
     1441 aaattgatgt agcttctctt caaaaatcta tagaaaagaa aataaaggag actacagtta
     1501 ctcaagaaga ttatgcaaat attgaaacac aacttaatac agctaatgaa gctattaaaa
     1561 agtttgaagg aggtatgaca aaagaagatg tagagaatct aaaaacaact tatgaaactg
     1621 ataagaaaac tttggaagaa acctacaaaa aagaaattga agaaaaggac tttaattact
     1681 ggttaaatga tgcttttaag tctgttaaat gtagggatga gatagcgtta aaagctcatt
     1741 tagacatgga agcactaaga aatagtaaag acagacaaaa agcttttgaa gagcaaataa
     1801 accctttgaa acaggataaa gattatttgt ttaatgcaac actagaaggt gaagaaccta
     1861 aaatagatac tataacacca gggcaggagc ctaagataaa tgattttggt tttaatttta
     1921 ctggggtaag acctcatgaa aataataata aataggagga aataaaatgg cagcactaaa
     1981 ttatgcaaaa gaatattcaa atgttttagc acaagcatat ccttatactt taaactttgg
     2041 ggatttatat gtaacaccga ataatggaag atatagatgg actggttcta aaacaataga
     2101 aataccaact atatctacaa ctggaagggt agattcaaac agagatacaa tagcagtagc
     2161 tcaaagaaat tatgataatg cttgggaatc taaaacatta actaaccaaa gaaaatggtc
     2221 tacattggtt catccagcag atattgacca aactaactat gtagcatcaa taggaaatat
     2281 aacccaagta tacaatgagg aacaaaaatt tcctgagatg gatgcttact gtatatctaa
     2341 aatatatgct gattggatag ctttaggtaa aacagcagag acaactgctc ttacaacagc
     2401 gaatatctta gaggtttttg atagcctaat ggaaaaaatg acagaagcta gagttcctgc
     2461 tgttggaaga atattatatg ttacaccagg cgttgatact ttgattaaga atgcaaaaga
     2521 aatacaaaga actgtaaaca taaaagatgg aggaacatct ttaaatagac aaactacaga
     2581 tattgattca gttaaaatag ttaaagtacc atctaatctt atgaaaacag tatatgattt
     2641 tacaactgga tggaaagcag gtgcaggagc taagcaaata tttatgtctt taatacatcc
     2701 aagtgcaata attacaccag tttcttatca attttctaca ttagatgaac caagagcagt
     2761 tacagaagga aaatatttgt attttgaaga aagttttgag gatgtgttta tattaaataa
     2821 aaaagctgat gcaatacaat ttgttgttga aggagctgga gcataatggc acaagtaagg
     2881 aaattaaata gaatattaac tatagaagag tgtaaaatag atgatttctt agagatggga
     2941 tatgatttga tagatgaaac tggtaaggca ctaaagtatg gcaagtcatt aaatgtaaaa
     3001 gatttaatag ctgaaaataa tattttaagg tcaaaagttg agtctttaga agaagaaaat
     3061 aagcagctta aagagaaaaa taaacttact aaaaagtagg tgaaaattat ggaaaataat
     3121 aggattgatg atatagaaag aagacttcaa ggatttggat atgttctaaa gaatggagat
     3181 aaatggttaa tagattttgt aagagaaaaa atagaaaata ttattaaact agattgtaat
     3241 ataggtgaaa ttccaaaaga gttacacaat atagaagtag atatgatagt tggagagttt
     3301 ttatttacaa agaaaaatat gggtcaatta gatatagaaa gtcttaattt tgaagctgta
     3361 gaaaagtcta tatcagaagg tgatacaaag gtagattttg ctataggaag tggctctcaa
     3421 acaccagaac aacgctttga cactcttgta aactatcttt taacttatgg caagaataaa
     3481 atattaacct ttaggtgctt aagatggtaa gtaaaactag aaaagcaata gaaatgttat
     3541 atagagataa atgtactata gttgagtatc agccaatcaa agaccctgta acaaaacgaa
     3601 ctaacaataa agaagtgatt gtattagaaa atcaaccatg taaactttca tataaaaata
     3661 tagtttctgc tacagaaggg aaagtagcta agctagagca aactattaaa ctctttatat
     3721 ctccagatat aagcgttaaa gcaggttcaa aacttattat aactaatcaa aataatatta
     3781 caaaagaata tataagaagt ggagaatctg ctatataccc aaatcatcaa gaaattgtat
     3841 tagaattact agaggataaa gcgtaatggg tagaggtgga agtgttaatt ttaaagaatt
     3901 gaaaaagttg caaagaaatc tacaaaggtt ggaagaaagt caactcgata agttttgcag
     3961 agattgtgca aaagaattag cagcaagatt aattggcaaa gctataaaac gtacaccagt
     4021 tgatacagga gtgttacgac aaggttggaa tggtgtggct tatgcaaggg cacttccagt
     4081 cattaaagct ggagatactt acattataga aattattaac cccatcaaat atgccagttt
     4141 tattgaatat ggtcatagaa ctagaaatca taaaggttgg gttaaaggta gatatatgat
     4201 gactatttca gtgcaagaat taagaggtca agtagatgta ataatagaaa agaaattaat
     4261 aaatttatta aagaaggtat ttgatgctta ataatattat agatggaata tcagtaaaat
     4321 tagataaatc atttggagag agttatacaa tttatagtga agatgtggag caaggtataa
     4381 atgaaccttg tttttttatt gttcctttaa atccaagcaa agtatcatat ccaagtggca
     4441 ggacattaaa aaagaactct tttgatgtac attattttcc caaaagtaat gataaatcat
     4501 ttgaaataaa tgaggtagct gagatgctac tggaggaatt agagtatata gaaattgatg
     4561 gagatttagt cagaggtaca aatatgaact ttgaaattgt agataatgtt cttcatttct
     4621 ttgttgatta taactatttc actataaaaa gcaataatac agataagatg gacacagtag
     4681 agttgtttgg tggtctaaag agaggtgata aacttgagta aaacattaag taaagaagaa
     4741 aattataagt ttactaagga gcaaatagtt aattctaaaa ggtatgtaaa tagaaaagat
     4801 ttattaaatg caatcttaaa ggaagatgaa ttgtattctt tatcagaagt ggaagatatg
     4861 ataaataatt ttataaaagg agtgagttag atggctttag gtggaggaac attcgtaacg
     4921 caaaataaaa tacttcctgg tgcatatata aattttatct cagctaagag ggcaactagt
     4981 tcattatcag atagaggtat tgttgcaatg cctttagagt tagattgggg tattgatgaa
     5041 gaagtattcc aagtaaccag tgatgatttt gagaagtatt caactaagta ttttggatat
     5101 gattatacac atgataagtt gaaaggtctt agagatttat ttaaaaatat aaggttggga
     5161 tatttttata aattgaataa aggcgttaaa gccagttgta ctatagctat agctaagtat
     5221 agtggaataa gaggtaatga tttaaaagtt atagtaacaa caaatataga tgataacact
     5281 aaatttgatg ttgtaacttt gctagataat aagaaggtag atactcaaat agcaaaggtt
     5341 attacagact tgcaggacaa tgactatgta atttggaaga aggatgcaac attagaagca
     5401 agtgcagggc tggtatttac tggtggaact aatggcgaag ctgtgacagg agcagaatat
     5461 caagctttct tagataaaat agaaagttat tcatttaatg cactagggtg tttggctaca
     5521 acaacagaaa ttaaaagttt gtttgtagag tttacaaaga gaatgagaga taaggtagga
     5581 gcaaagtttc aaactgtttt atataagaaa agtgatgcag attatgaagg tgtagtgtct
     5641 gtagaaaata agattaaaga tactgaatta ttagaatcta gcttaattta ctggactact
     5701 ggagctatag caggatgcga cataaacaaa tctaatacaa ataaaaagta tgatggcgag
     5761 tttgatgttg atgtaaatta cacacaaata cagttagagg aagctttaaa aactggtaaa
     5821 ttcatattcc acaaggttgg agatgaaatt catgtgttag aggatataaa tacttttgta
     5881 tcatttacag atgataaaaa tgacgatttt tcaagcaatc aaagtgttag agtgcttgac
     5941 caaattgcta atgatattgc aactttattt aatgaaaagt atttaggtaa agtgccaaac
     6001 gataaggctg gtcgtatctc attttggaat gatgtagtaa aacaccataa ggaattagaa
     6061 aatattagag caatagaaga ttttaaaact gaggatgttt tggtggaact tggaaacgat
     6121 aagaaaacag ttatagtgag tgatgcagtt aaggttataa atgctatgag taagctttat
     6181 atgacagttt cagttagtta agagaggagt gtgataatag atgtctaaaa atattactat
     6241 gaatgttaga gatgcaataa gtggttctaa agctgaatgt tttgtaacaa taaaaggtaa
     6301 aagatataat tttatgcaag ctattaactt agaagctaag atggaaaaaa ataaaagcga
     6361 gatgccaatc ctaggtagca ttacaaaagg aaacaaaagt acaggaagta aattttcagg
     6421 gagtgcaaca ttttattata atacttctat ttttagagag ttgttatatg aatataaaga
     6481 aacaggcgag gatatttact ttgatatgca aattaccaat gaagacccta catcaagtgt
     6541 aggaagacaa actataatac ttgaagattg caacctggat agtggaataa ttgctaagtt
     6601 tgatgctgat ggcgagtatt tggatgagga tgtagatttt acttttgaga attggagaat
     6661 agttgagaag ttcaatatag caaatggtat ggaataagat acacatttat aaattataga
     6721 tgtgtatttt ttatttacaa aattaggaga tgattaaaat taaagataaa tatgagataa
     6781 aagattcaat ttcttttgat tatagtaaaa aaagacatat agaagaatgt gttagtgaaa
     6841 tatataaaaa agcagggaaa tatcttatag atattgctga taaactggca cttgatacaa
     6901 ttgaaggttc atcattaaaa ccaattacaa taaaaatcaa actagatgag gatggcattg
     6961 caacaataga aaaagaaaca aaatatttaa tcatggaggt agaataatat gggagattta
     7021 aacgcttttt taagtcaaaa tgcaataaaa gtagagaata gaaagtatgt ggcaagtgaa
     7081 aggtttgtag gtgaagatgg aaaaccagta gaatgggaac ttaaagcaat agattcagat
     7141 agagatagac agttaagaaa aaattctgct atcagagttc ctgttatagg aaaaaaagga
     7201 aaagctacag gacaatacac tagtgaaaca gattttaata cttacacttt gaaattatgt
     7261 gtggaaacta tagtatttcc agatttacat gatgcagaac ttcaaaatag ctatggtgta
     7321 atgggagcag aggaactatt aacaacaatg ttaactcctg gtgaatacac agacctttca
     7381 agtgaggtag gagaagtgaa tggttttgat aggacttttg aagataaagt agaagaagca
     7441 aaaaactaat tgaaggaggc gattatgatg ctagtgtagc ccattattgc cttcataaat
     7501 tcaaatggaa accacatgaa tatacagatt tgccagacta cgagagagca tttgttgctg
     7561 cttctataga tattaaagta gaagaagaaa taaaagaaga aaaaaagact gctaaagaag
     7621 ctagaagaag tagaaggaga taaaatatag gtaaaatatg taagaattat atgttataat
     7681 atttttagca agaagatgta atctacaatt tatagagtgg agttcataca aaagattatc
     7741 ctcccaacgt atagaaggga ggtgaatatg tatggataat tttttacaag gtgtactagc
     7801 aagtttagtt gccagtttaa tagtttactt aactagtaag ttatttaaaa aagtaaaaag
     7861 ccactcaaac acgactaaga gtgactttga ttttaaactt acaatcaagt ttaaaagaaa
     7921 taaacattaa tttttagaac ttcactctag tttcaaatag attgtagttc ttcttgcttt
     7981 tattatacca caaattagaa aaaatattgc ctataatatt tttatagtca ataaaaagat
     8041 gaaattttta taacaaataa taaaaacttt attataaaac tattaatttg caaggtatct
     8101 ataaatgagt agatattttt ttacttatat aaaatatttg aaatgatagc atattcctgt
     8161 tttgggaacg tggttaaaac tcaaactata ttactagtat tatattaaat atttagcatc
     8221 aaaattaaat aaagaaaaga aagcacttac tttttggtag gtgcttttgt tttgctcaaa
     8281 ttggtcggtt gagtaaaata attagaaaaa attggtataa gttattgact tttgagttcc
     8341 aaaactatat aatttaatta tggaactcaa aagtgaggtg ataacatgag tcctaaaaaa
     8401 ataggaagac ctgttgtagg aagccctaaa actaatgata ttaaggttag agtagatgat
     8461 gaaactaatg aaaaattaga tgaatactgt aaaaaaaata accttacaaa agcagaggct
     8521 ataagacagg gaatccattt acttttagag aaataacaaa aatagagcat tctccccgac
     8581 caaagattga atgctctatt tccaacaaag aagtaatctt ctatatgaaa tattctatca
     8641 tataagggta cttctttcaa atcaatttga ggaggaattt atatgaaaac taatttagaa
     8701 ttaatcaaat cagaaaactt tggagaagta gaatgtaact tttatgaaaa tgatactaaa
     8761 gatatattaa tgactaggga acaaatagga atggctttag agtatacaga accaagaata
     8821 gctataagta aaatacatga acgtaacaaa gatagacttg ataaattttc aactgtagtc
     8881 aagttggtta cagttgaagg gaatagagaa gtattaagag atactatagt ttataataca
     8941 aaaggagtaa tggagatatg tcgttttagt agacagccaa aggctgatac ttttatggat
     9001 tgggtttggg atgtagtaga atctatattc aagactggtg catacataac taacaatgcc
     9061 aatcccgaaa aactaagaga aaaagcaagt gagattgaaa aattacaact ggcttataac
     9121 agtacatcta tgttaaaaga actattggat gatgcaggat ttgacaataa atctaagtta
     9181 cttacagcaa agacattata taagaaagca ggcattgatt taccaataga gatagatgaa
     9241 gaagagcatt attttgacac aaaacaaata gcgtctaaat tgaaaatata ttctaaaagt
     9301 aataaaccag ctcagatggc tgtttgtgag attattaaaa agataaaatt agaagaaaat
     9361 gaagtaaaag gagtttggga gactaatggt tcttggactg gcactgtaaa taagtataca
     9421 aagagtgtaa tagataaagt gagaaattgg atagaggaaa ataatagacc tactaagata
     9481 caaggtgaga agaagaatta tcatgtggtt tataaaattg agtaaattct attgtattaa
     9541 ataatatatt ttagtttagt tttgaggggg attaatacaa tgtgtaaaag tttacttgat
     9601 gattacaatc taaaaactga taaagagaga aaagatttta taaagtttgc tatactatta
     9661 tataagttgg aacaggacaa caaagaaaag ttttatgaat atacagagat attgagaggt
     9721 attcttagag aacaacaaga gagagaaaat aagtaaaata atatagataa agcacttgga
     9781 tattatgatg tttcaagtgc tttatatggt ataaaatggt ataatggagg taggaaatta
     9841 tattaactaa gtggtatgta aatataaacg cgtatttaac atatttttta ttatcatcac
     9901 gttttatatt aactaagtgg tatgtaaatc caattacaaa cgatttattc atcagttcag
     9961 caaattattt tatattaact aagtggaatt aaaattaaaa ataatcaaaa aacacttgct
    10021 tatggtaggt gtttttttat tgaaaggatg tgattataat gtaaaaattt tactaatata
    10081 gtataatatt gttaaagaat ataatttagg gggatattat attatgaaga aaaaagtatg
    10141 ttttttattt tctattctta tagttatctg tttagctatt gtaggatgtt ctaattcaga
    10201 aagtccagag gagagtagta aaaataatga accaaaaaaa gaagaaaaaa aggataaaga
    10261 agtagtcata ggagagaaaa ttatttcaga taaaatggaa atcactatta ataatattga
    10321 attctcttat gatgttttac caaaggttaa ggaaagttta tatacacatt atcctgctga
    10381 gtcaggtaaa gtatatattg atattgctgc tgatataaaa aatactcaaa aacaagagtt
    10441 aaattgttca gatttactaa ctattgaagc aaattataat gatgggtata aatattcatc
    10501 acaaacaata gtagaagatg aaacaacagg ttttacttat gataatatta ccagtatcga
    10561 ccctttagaa acgaagggag ttagatttat aattgattgc cctgatgaag taaaaacaag
    10621 tgataagcca ctggtattaa gttttacatt tgatgggaat aagtatgttt ataaaatgaa
    10681 gtaacttatt taaaatgaat atattatagt gataaggaga tattttatga atgatacagt
    10741 agaatatata ttttgtagcg aatgtggatc aaggtgttta aaaggaagca aattttgcaa
    10801 tgaatgtgga acaaaaatag atgatgtaaa aatacaaaaa gaaagcatta ctaaagataa
    10861 ggaatatgat ttaaaaggtg ttgatttaca acatataatg caagaaacaa gttttataaa
    10921 agcttcatct gtaagaagat tgaaagaatt aactggaata gagttggatg attgtagaaa
    10981 aatattagaa gaaccatatc aaaaatatta taatgaaaat gctgatatgc ttgaagaaaa
    11041 aagaaaaaaa gaagatgata tatcaaaaat tagaaaagct aaagaggaaa agaaagaaaa
    11101 agaaaaaata gcttgttgcc ctgcatgtgg atcaacttct ttgacagcac ataaaaaagg
    11161 ttttggcata ggaaaagcta taacaggagc tactatagca ggaggcattg gtctcgtagc
    11221 aggaaacata ggagcaaaga aagttagagt tacatgcttg agctgtggca agcagttttg
    11281 ggcaggtaaa aaatagatat attaagatta taataaagaa aaggaagcgc ttactaaatt
    11341 agttagtgct ttttattatg tttaaaataa ta
//
LOCUS       DAKBNN010000127        11361 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000127 DAKBNN010000000
VERSION     DAKBNN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.128"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            310..462
                     /locus_tag="OUB02_002676"
     CDS             310..462
                     /locus_tag="OUB02_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886420.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(754..1458)
                     /locus_tag="OUB02_002677"
     CDS             complement(754..1458)
                     /locus_tag="OUB02_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886421.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1449..3056)
                     /locus_tag="OUB02_002678"
     CDS             complement(1449..3056)
                     /locus_tag="OUB02_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HCT8886422.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3310..4488)
                     /locus_tag="OUB02_002679"
     CDS             complement(3310..4488)
                     /locus_tag="OUB02_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886423.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4714..5208)
                     /locus_tag="OUB02_002680"
     CDS             complement(4714..5208)
                     /locus_tag="OUB02_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCT8886424.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5209..5970)
                     /locus_tag="OUB02_002681"
     CDS             complement(5209..5970)
                     /locus_tag="OUB02_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCT8886425.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6672..9506
                     /locus_tag="OUB02_002682"
     CDS             6672..9506
                     /locus_tag="OUB02_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886426.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISSKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDLTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9549..10793
                     /locus_tag="OUB02_002683"
     CDS             9549..10793
                     /locus_tag="OUB02_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCT8886427.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         4088 a         1821 c         1416 g         4036 t
ORIGIN      
        1 gatataaaaa gacaaaaagg gcaatttttt tatatcaaaa aaaatgttaa attgatatta
       61 tccatataga tgctatattt aacaattcta cttacttccc ttacacttat tagcatatag
      121 ttattaagag tttgttgcaa aacaacactt agcacacttt aaaaagtgtg gtctaataag
      181 taatcaacga agaagacata ataaagcata aaatatgata tacttttcca cgtcatcaaa
      241 actgaggatt ttattattaa tatatgataa aatatgctta ataaattaaa ttttaatggg
      301 agaatagtta tgaaaatatt tagtttcttg ttgtttgtag tgggaatatg tttttgtatt
      361 gctggattta tggggcattc ctcagctttt tctggtgcaa cattgagttt ttttgcaact
      421 acaatgatag ctgtaagaca agtcaaaact aagaaaaaat gaaataggca aatcctaact
      481 ttacaagtta atattacata agtaattagt ctaaaattag atactttttc tattacagaa
      541 atacatcatc cactttgtat tctcttaaaa aaataaaata gctatgtaaa ttcaagatta
      601 gatagactaa ctctctatcc atttcattgt aaaatataca tagctatttg tattcgattt
      661 aaaatactta gattttctta cataacaaat aatttctatc tgattattac tcatattcat
      721 ttagtaatca ctcagcaact aatcatttag tatctattca tccgccatac ggtaacctac
      781 cccaacctca gtcaaaatat attctggctg agcaggattc ttttcaattt tccttcttat
      841 acttgccata aaaactctaa gcgactgatt ctcactttca tagtaatttc cccaaacttt
      901 atctattata aacttatggg ttaagactct tcctgaatac ttagacatca actcaataat
      961 cttatattcg attggagtta aatgtatttc ttcaccatta attgacacaa tatgattttc
     1021 ataatctatc tttaggtttt taacttcaaa agactttgga gcatcattat ttatattatc
     1081 ctgttgagct ttatttctaa gagccacacg tactctagcc aaaagctctg ttacactaaa
     1141 aggtttagtt aaataatcgt ctgctcctcc atctaaagct tccaccttat ccctctcatg
     1201 ctctctcgct gaaacaacaa ttattttatt agtctttgca catgccctaa ctcgtcctat
     1261 tacctcaatc ccatcaatat ctggtagtcc taaatccaaa attatcaaat ctgggtcaag
     1321 agaatgtata agtgatatag ctgttcctcc attatctgtc tctacacacc tataattttg
     1381 agtttcaaga gatactttta taaatttaca aattggctta tcatcttcta caattagaac
     1441 taatggcttt atttccactt gtctttcccc tcctcatcat acataggtat gctaaatcta
     1501 aatgtagctc ctccagaagg attattaaag gcgacaattt ctccaccatg agcttctact
     1561 atagatttac atatctccaa tccaagacca atacctcttc tttcaagatt tctagactta
     1621 ttcttagtat aaaacctatt aaaaatattc ccgatatctt catttggtat tccttttcca
     1681 ttatctgcaa cttcaaatag tacttgattt gactcttttt ttacagttag ttctatctta
     1741 gaatttttag gtgtatgcct tatagcatta tcaattagat ttactaacac ttgttctatt
     1801 aataatacat ccacatgaac gattattatc tcatctggaa tatttgtttt tatgtctcta
     1861 ctattggcaa actttttgac tcttaatata gattcagcta ttatttcatc aacaacctct
     1921 ggtcgttttt caatatccaa ttttccttca tctattctgg tcatactaag tatattctca
     1981 acagaatgag ttagccaact cgtatcttca tatatatttt tcaataattc tactctagtt
     2041 tctttatcta tcacatcatc attttcaagt attgttgaag atgaacccaa tatacttgta
     2101 agtggagttc ttaaatcatg agaaattgac ctaagcaagt ttcctcttaa tctctctctc
     2161 tctgcctcaa gatttgcttt tttatttttc tcaaataaat tttctctatc aattgccaac
     2221 gcaacctgtg ttgaaactgc ttctaatagt atcttttcat tttctgtaat aatatctatc
     2281 ttaaaacacg caacacctat aacaccaaga acatttgatt cttgaccttt tatagggaaa
     2341 taatatgcac tgctgttttt acaatatttt gtcccaattc caactggatt acctgtccta
     2401 aaagcttctt ttatagcttc cttttctaat actgaatgga atatattctc acttccatca
     2461 ttgttacaaa tataaatact tggcttacct agattattat tctcatctgc aatagtcact
     2521 attacagttc tatttaatat atttactaaa ttagacccac aaaattcaat aatttgattt
     2581 ttattttttg ctttaagcaa cgttttgtta ttctcataga gcaatcttat cattttttct
     2641 ctcttatgag aaagtctcac ttctcttttt atcctagaag ttaaagtact tgttataatt
     2701 gctgccatta gcataataca aaaggtcata ggataatctt ttctataagc taaaaaagta
     2761 tataatggat gcgtaaagaa aaaattaaat gatagcactc ctattactga agaaagtatt
     2821 ccaaaaaaat atccataggt tatacttgta gcaaatagta cacctaatat gtatactagt
     2881 ataatattag attcattaaa acctaccttt tcaaaaacta ctgatattcc agtagcaatt
     2941 gtcataacca atatagtttt tatgaagtcc ttaataatta tctttttact gagatagcca
     3001 tcttcatcat aatcatactc ttttaagtct tcatgttgta gtcgttcctt tttcatttac
     3061 tacaattcct ccttattgtt actgtttaaa ttggctctat tttatataat acaattttat
     3121 cataataaat tcaatcaata catttaatta atgtacttaa attactaaaa ggtgctgtct
     3181 taaaatgaaa ataaatctct atttcaaaac agcacctata tatttaaatt actaatttag
     3241 tttttacata cttaaatcaa tagttttatt caatttcata aattataata cttcaattat
     3301 aaaacatttc tatccaacaa gcataaatat aaagtgggca catatacctg cacataaacc
     3361 acctatagta tgcgaaagta tcgctttacc agccaatggt cttgcatcta aagcatccat
     3421 catacctatg tgtgtactta aatatccact ccaacacata cccattgctg tgaatactgc
     3481 tatatcatta ggagttatag cgcctgattt tataaactct ggtactaaag atattgctgc
     3541 tccaacagct cctaatgcag ttacagggaa tgctattgcc tctggtgatg tgaatccaaa
     3601 taatggttct attatgaagc taatcttatc accaatagca ggtaataatt ttataccttc
     3661 atatgcagct cctgtgtaaa cagcttgacc tgtagcaggg tctgtagatg gtccaaaagt
     3721 taataacata actaatgtac aaactactaa aactcctggt attatagcca tacccatttc
     3781 aacacctaac ttaccacctt caagtatagc atccaatgtt ctttggaata cattaccatc
     3841 tcttatttct ctaaactctt caagttttgc acctttatca tttatttgct tttcagtatc
     3901 atttttaggg tcataattat aatacttttt agtaaaagtc aacataattc taacactaat
     3961 aatactacct attattgccg caacattacc tattatagct ggtagtacat attctttacc
     4021 ttgagctatc ataaaagttg ttacaataag tcccatacca aaggctgttc ctaaattaca
     4081 taaagcaggt acttgatact tcttaaaata ttgtgtaaat gttttgtctt tagcaaatgg
     4141 tataatagct ggattatcag ataaatatgt tgcaactact cctgctatac tagcaccagg
     4201 taagccccat ataggtctca taagaccctt aaatatctta ttaactaaag atattactcc
     4261 aaactctgaa agcaatgcac taagtgcacc tgcaagaact gccatagcca ttattaaaaa
     4321 tactgtgtct aataaaagcg catgtgctgt actcattatt actttaaaca tcattcctgc
     4381 acccatgatg cttcctacat atccaaatcc taccgccaat agtacaataa atacgaaggt
     4441 ttccatactt actgccttga ctcttctctt atccgacagc ttttccatct tgaaatcttc
     4501 ccccttttaa ttttaaatta aggttcctaa cattggtaca aattttttgg tacaaactga
     4561 gttctaaaac ttacactcgt aatgcaagtt gtcagttttt tgaaataaaa aaaaattacg
     4621 tttatagaat aagaatatcc tctttaattt ggatactctt attctatttt atgttattat
     4681 attatcatcc ttttgtttaa aagtcaattt tttctatttt tgttgtttaa ttttatttat
     4741 actcttttta tatttttcat tttcttttat ctttgtaagt actccatata agttgacttt
     4801 tctaaataca gtagttataa ataccaatcc taatgcaatt gaacttgcac acgataatgt
     4861 actcacccca tattctacag cttttatata attattggtc aaaacagaat acatagtatt
     4921 atatattcca taacctggta ctaatattat taaacagcaa acacttaatg tagttgctgg
     4981 agtcatataa attcttgcac ataactcaca gtatatcgca aaacaaactg tagctataaa
     5041 aaaacataag ttttctgtta ctcccatatt taaaagaaat ttatacgaaa accaagctat
     5101 acttccacct attcctgcta gtattaagtt ttttcctttc atattaaata gcactccaaa
     5161 gcttaaggca gtaaaaaaag ctgctattac ttctataaac aaatgcactt atatcccccc
     5221 taacttgaag tacaatccaa gtaccaagaa cattcctact gctatagata cacctacaaa
     5281 aaatacttct gctaattttg caagaccaga aatcaactgt ccatctaaca aatctcttaa
     5341 tgcattagtc aaagcaagcc cgggaactaa gagcattatt gctcctgcta ttaaatggtc
     5401 taacgtcttt atgaatccga gctttaaaat taaaattgaa caagaagcta taataactgc
     5461 tccaattgca tttacaaaaa agtcattaag ggaagattta cttaaataca taataacaaa
     5521 cttagtcaga atcccaacag taaatgcagc tataaattct tccacatttc ctccaaataa
     5581 tatcgaaaaa gttgctgctg caatacctgc aaaaaatatt gttattaaat ccgagtaata
     5641 gttatcttca cttatttcat ctaaaatttt ctcacataat tcaatatcta aattatttat
     5701 acttattgct cttgataatg agttaacttc atgaaccaag tttaggtcta ctcttcttga
     5761 gcttattctt ttaattgctg aatgtaattt cccatctaca aatacagatg ccattacagc
     5821 tgttggagtt gcaaatacat ctacgtggtc tattccaaaa gattgacata ttctagctat
     5881 cgtttcttca actctatagg tttctcctcc actttgcaac atagctttac ctgcatttga
     5941 ggaaaagttt aaaattctat ctatatccac aaaacatatc tcccattctt ttatagtatt
     6001 attcatattt taatacaaga tattatttat ttaaactatt ttaaataatt catatccaat
     6061 atttacattt tatgaaaaaa cttgtttttt atgcttgtca aaatcaaatt ttaagcttgt
     6121 ttacaccata aaatggctga attaatattt gacaaacact ttcaaccatt tatataacct
     6181 tctaaattgt ataacttttt actacaaata aaattatatc tcttttaatt ctttctcttg
     6241 agtatttagc ttatagtaac tttatattta tatatgtaca atataaaaca ttatttttat
     6301 gatacaaaat ataatttgta aatgtatata tgtattaact gtgtatatat aaattattct
     6361 tgaccttatc agttttttca tatatcttca tctataccaa ccatatatta ataaatgata
     6421 ttacatagac ttaagtatat taaaaaactt aaatttagtt atttaacaac ataatactct
     6481 aatttaatct ataaaaatcc atacttaata taatgcaact cttattttaa caccatctat
     6541 tacacaaaac aacatattaa aaagtaaaat acaaaatatc cactataaac cttattttaa
     6601 taccgaatat atattataat atatttaatg tagcttaaat atagtttaaa cataaagaaa
     6661 ggaaacggta tatgctaaga aaagataaag ttctattatc tctacataaa ttatgtgaaa
     6721 atatgacttt aaaagacctt caatcagata aaacaggatt cactacaata gaaattgcta
     6781 accattcatc tcttgataga agtaatacca gtaaagaatt aaataaccta tacaatgaaa
     6841 aaaaagttct gaaaatatct agtaagccaa ttttatttct acatgctagt attatagaaa
     6901 atttaattgg ccgtactttg aacagtgatg aattttctat aaataattgt aatgagctac
     6961 ttaaatcaag taaaaatgac tcagaagata ttttttcaac attacttgga catgattcta
     7021 gtcttaaact acctatagaa aaagctaaat ctgctatact ctatcctcca aaaggtctta
     7081 atacgttact cataggacct actggtgttg gaaaaagtac atttgccgaa acaatgtaca
     7141 aatatgctgt agaaagtaaa atcttttctt ctgaaagcaa attcgtagta tttaactgtg
     7201 ctgaatatgc agaaaatgct aacttattat taagtaattt atttggttat gtcaaaggtt
     7261 cttttactgg tgctaataag gataaattcg gcatagttgc acaggctgat ggaggcatat
     7321 tattcttaga tgaaatacac agattaccac cagaaggtca agagatgctt tttcttttga
     7381 tggacaaagg catatacaga cgattaggag aatctgacat tgtgcacaaa gctaatgtct
     7441 ttattgtgtg tgccacaaca gaagatatac attcttctct gcttgggact tttttaagaa
     7501 gaatccctgt tacaatcaac ctacctgctt tacaggatag gtctttaaaa gaaagattat
     7561 ctcttattaa atacttcttt attatagaat caaaatacac caatgcaact ataaaagttc
     7621 acaaagatgt aattaaatca ttactccttt atgattgttt tggtaatata ggtcagctta
     7681 aatctgacat acaacttatg agtgcaagag ccttcttaaa ttacaaaact ggacttaagg
     7741 ataatataga aatagattta tcccttgttc ctgattacat ctatattggt cttttaaaat
     7801 tgaatgaaaa tcgtagtaat ctaaataaat tatcagaact tgattatatg cttttttatg
     7861 aatttaatgg taataactta gaagttgaat atacaaaaga actatctaaa tacaatatag
     7921 atacatcaaa caatggattt aaatactcta cagagattcc ttatctggaa aatgatataa
     7981 ggagtatgtt tataaattac atggaaaagt actctacaaa attactttcg atatcttcag
     8041 taactcattc tgattataca agtaatgaga tttttaaagt aatcaatcca aagatttact
     8101 atgctgttga acattcttta aaagaggctg aaaaaatatc gagaaacaaa tacacaaaac
     8161 aaacttatat tgctctttca atgcatataa gtgcacttat agaaaatata aatcaagaca
     8221 catataatcc taagtacaat ttatataatg ataaaatttt cttagataaa gatttaaata
     8281 tagctaaaat agttttaagt ataattgaaa atgacttaca aacatcttta cctaaagatg
     8341 aagtaaaatt tatatccatg tttctaaata gcatgtatct tgaaccaaac aatcaatctg
     8401 aaagtatagg agtcataata cttgctcatg gaaattcaac agcaagcagt atgtgtgatg
     8461 tagcaaattc attgcttggt acaaaccatt gtaatgcaat tgacatgcct cttgatgtaa
     8521 aagtaagtac catacttgat aaaactatag agttggtaaa agaatgtgac gaaggtaagg
     8581 gtgtacttct tttaacagat atgggctctc ttatagcttt tggagaactt atttcagaaa
     8641 aaacaaaaat caaaactcgc tcaattgaaa tggtatctac tccaatagtt ttagaagcag
     8701 taagaaaaag tacactacca gaaatgactt tggatgaatt agctaattcc ttaagctctt
     8761 taaatccata tattggacga gcagcaatcg atttaacaga aataaactat cctgaaaagt
     8821 caagagagta tgtaataata actacttgta taacaggata tggttcagct atgaaagtag
     8881 cagactttat aaattcttca cttaaaaata ttagtaaata caatattaat ttagttcctc
     8941 ataatataga aagttttaaa atgtataaga ataaatttag taatactaaa attctggcag
     9001 ttgttggaag tgtagatttt aatgtagcca acaccccttt tatacacata gaggaactac
     9061 taaatggata tggcctagat atgctaaaca aaataataga aggtacattc aatgccaata
     9121 gtattacaaa tataaaagaa acaaatctat catacaacag tttaataata aactttatga
     9181 cccaaaactt agagcttttg gactctgtta aattgttcaa ctatgtcaat tcttccttat
     9241 ttacaataat agaattaata gatgaaaact taaagagcaa atttaagcta ggatatatac
     9301 tacattgctc ttttatgata gaaagatgtt ttaaaaatac aacttttcca tataataaca
     9361 ttactgaatt aattagtaaa aatcatgaac tatatagttt aatacgtgaa gcctttaagc
     9421 caacagaagc ttattttaat attttgatac cagacgaaga aattgcttat ataatagaca
     9481 tgattaatga gtatctaaat aaataaaatt tatagcactt attttgtgca tattaattaa
     9541 tatatgaaat ggaggacgag attttgaaag gaaaaataaa acagttaaca atacttgcat
     9601 taatttttat atttataact cctgtatttg catttgcaga tactccacca gtaccaaatt
     9661 cttcaagagc agcactactt atagaccagg aaactaaaag aatattattt gaaaaaaaca
     9721 ttgatgaaaa aatgccatta gcgagtttaa gtaaaatgat gaccttttta ttggcaatag
     9781 aagcagttga taaaaatcaa gttaaagaga ctgatatggt gaaaatagac aagtctacag
     9841 cttcagtagg aggttctact tgtaaactta aagatggtga tgaaatatct ctaggagagc
     9901 ttatgcaggg tctaatgcta gtatctggaa atgatgctgc tatagccata gctaaacata
     9961 ttggtaaaac tgaaaagaat tttgtaaata tgatgaacaa gaaagcagaa gaaataggaa
    10021 tgattgatac gtattatttt aatccaaatg ggcttcctat atatactgac ccagaacata
    10081 aagaacctcc tattgaaaat atgtcaactg ctcacgatat agttacactt ggaaagtata
    10141 tgtacgacca ttatgaaaat caagttacaa gaataaccac tatgcaagta tataatgata
    10201 ctaaaaagga ttttacacat tataacacaa atcctctgct tgtttcagta cctggtgttg
    10261 atggaataaa gacaggatat acagataatg ctggatattg ccttgcattt tctatgatgg
    10321 tacctaaaga tgctaaaaac gaaagaaatc atagattgat aggtgttgta cttggtgatg
    10381 gtaacaagaa aaatagaatt tcttcttctg ctactttatt gaaatatggt aaagataatt
    10441 tccattctaa aaagatagca cataagggtg atattataga gactccttgt gttgatggta
    10501 ttgatgattt taagataaca gttaaggttg ataaagattt gtatggtgtt gtttcagata
    10561 acgagaatat caatcctaag gtagtgttta aaaatatgaa ttatcctatc cataagggag
    10621 atattgttgg tgttgcaaag tattataatg actctggtaa atttgttgga agtgttgatg
    10681 taaaaagtga aagtaatata ggatgtattc ctttgaagga taagattaag attaaggttg
    10741 ctaagataaa taaaaaactt gagattaaaa attctgtttg ttttaaagca tagtaaagca
    10801 tattattaaa agggtatgtt aaatttataa agactccact ttgtggagtt tttttgtttt
    10861 ttacaaaaaa ttaaaatagt ggcaacaatt gaggatgtga gggagagttg ccactatttt
    10921 gtatagggta ttatattatg tttttaagct agttttacta tcttctatgt ttatataata
    10981 agccttgtat atgaatttaa ttgataatta atatgaaaat tatgtgaagt ttttaattga
    11041 tgtaaagtaa tagattctat ttagacactt aaaattataa taaaacatct taatttttat
    11101 atttatacta attaattcta gtataaatac ttattaaata cctttattca aacattattt
    11161 cagatttata ctattcttat atgttaaaag gctgtagaaa aattatttta aaaatcctac
    11221 agccaatact agtatcatca agaaaattta catcttatta aactaatttt tattcatatt
    11281 tcattattta ttttaatata ttaaataaac ttgatagccc agaagatatt tttcctattg
    11341 ttgatattgg atctgacgca t
//
LOCUS       DAKBNN010000128        11290 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000128 DAKBNN010000000
VERSION     DAKBNN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.129"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..188
                     /locus_tag="OUB02_002684"
     CDS             <1..188
                     /locus_tag="OUB02_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886428.1"
                     /translation="NINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVY
                     NYIFKEIETKEVIKLIF"
     gene            214..786
                     /gene="folE"
                     /locus_tag="OUB02_002685"
     CDS             214..786
                     /gene="folE"
                     /locus_tag="OUB02_002685"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCT8886429.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            850..1641
                     /gene="folP"
                     /locus_tag="OUB02_002686"
     CDS             850..1641
                     /gene="folP"
                     /locus_tag="OUB02_002686"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCT8886430.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSYGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            1652..2017
                     /gene="folB"
                     /locus_tag="OUB02_002687"
     CDS             1652..2017
                     /gene="folB"
                     /locus_tag="OUB02_002687"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCT8886431.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVRVRVRKPEA
                     PVNGIYDYFGVEIRRARDE"
     gene            2010..2516
                     /gene="folK"
                     /locus_tag="OUB02_002688"
     CDS             2010..2516
                     /gene="folK"
                     /locus_tag="OUB02_002688"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCT8886432.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEKLIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            2497..3282
                     /gene="aroF"
                     /locus_tag="OUB02_002689"
     CDS             2497..3282
                     /gene="aroF"
                     /locus_tag="OUB02_002689"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCT8886433.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            3537..5327
                     /locus_tag="OUB02_002690"
     CDS             3537..5327
                     /locus_tag="OUB02_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCT8886434.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            5345..6511
                     /gene="rpoD"
                     /locus_tag="OUB02_002691"
     CDS             5345..6511
                     /gene="rpoD"
                     /locus_tag="OUB02_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCT8886435.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            6676..7365
                     /locus_tag="OUB02_002692"
     CDS             6676..7365
                     /locus_tag="OUB02_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCT8886436.1"
                     /translation="MKLTDRLLKIASLASDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            7379..8476
                     /locus_tag="OUB02_002693"
     CDS             7379..8476
                     /locus_tag="OUB02_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCT8886437.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            8539..9111
                     /locus_tag="OUB02_002694"
     CDS             8539..9111
                     /locus_tag="OUB02_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCT8886438.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKMGIRDYLKRLNI"
     gene            complement(9171..9638)
                     /locus_tag="OUB02_002695"
     CDS             complement(9171..9638)
                     /locus_tag="OUB02_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCT8886439.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(10024..10500)
                     /locus_tag="OUB02_002696"
     CDS             complement(10024..10500)
                     /locus_tag="OUB02_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCT8886440.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(10620..>11290)
                     /locus_tag="OUB02_002697"
     CDS             complement(10620..>11290)
                     /locus_tag="OUB02_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCT8886441.1"
                     /translation="KFKIIYLIPILFTLGMSIFIAFNYNQLPDSIATHWNINGSPDVF
                     IDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSRIKIDTNRIEESRVENIKYLNKIGYL
                     FLILMIIMTTQFFTTLLSIKTKLSTAMNITTLLVIIYLIATYINSPNLKFNSSYSPEE
                     DEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINFGNPLGKILYIAIALLLIFSLFSLIK
                     SLLL"
BASE COUNT         4539 a         1233 c         2066 g         3452 t
ORIGIN      
        1 ttaatataaa tgataaatgg gtaccaaaag acaaaaaatt gctaaaagtt ttaaaaaaag
       61 aaaataatga gctttttgaa ctagtgtcaa aagtaagtga aaattatgac tatcaaagac
      121 ttttagatgt ttataattat atttttaaag aaattgagac aaaagaggtt ataaaactaa
      181 tcttctagtc acaaatcaat ggggggagtt taaatgaata aagttgataa agaaaagata
      241 caacatgcag taagagaaat actagaggca attggggaag acccagatag agaaggtcta
      301 atcgaaactc caaatagagt tgctaggatg tatgaagaga tattctctgg tcttagtgaa
      361 gaaccaagag accatttaaa agttcttttt gcagatgaaa aacatgaaga gttagtattg
      421 gtaaaagata ttccatttta ttcatgttgt gagcatcatc tagttccttt ctttggaaaa
      481 gctcatattg cttatttacc aaaaggagga agacttacag ggctttctaa acttgctaga
      541 gtaatagata cattggcaaa aagaccacaa ttacaagaaa gaatcactaa aaatgctgca
      601 gatataataa tggaagaatt acaaccttat ggtgtcttag tagtagtaga agctgaacat
      661 atgtgcatga caatgagagg cgtaaaaaaa ccaggttcaa aaactgttac atcagcagtg
      721 agaggaatat ttgaaaagga cattgcgtct agagccgagg ctatgagttt gattactatg
      781 aagtagcaat ttattataga tataatcatt tattgagata aaatttaatt taagggagtg
      841 tcattaattg tgtttgatta tggtaaaaga acttatataa tgggaatatt aaatgtaact
      901 ccagatagtt tttctgatgg aggagatttt aataacttgg acatagcaat acaacatgca
      961 aaagatatgg ttgaccaagg tgcagatata atagacctag gaggagagtc tacacgccca
     1021 ggtcatagct atgtagactc agacgaagag ctaagaagag taatccctgt aataaaaaaa
     1081 cttaaacagg aattggatat accaatatca atagacactt ataaggcaga tgttgcagaa
     1141 gaagcattaa aattaggtgt cactatggta aatgatgttt ggggccttag aaaagataaa
     1201 aatatggctt ctgtaatagg aaaatatgat gcagaagtgt gtataatgca taatcaagat
     1261 gggacaaact atgacaaaga tataatggag tcaataaaag acttttttaa ggtaagtata
     1321 gaaatggcaa tgagttatgg tgtaaaaaaa gaaaaaatag tacttgaccc aggtgtagga
     1381 tttggcaagg attttgaaca aaatattgaa gtattgagaa gattaaatga attaaaagat
     1441 ttagggtatc ctattttgct tgggacatcc agaaaatcag taataggaaa agtacttcca
     1501 gttgagccta aaaaaagatt agaaggtaca atagctacta ctgttcttgg tataagagat
     1561 ggcgtagata tagttagagt acatgatgtt tatgaaaatt taatggcagc tagaatgact
     1621 gatgcaatat atagaaagta ggtataatat aatggataaa atattactta gtaatctagg
     1681 attctatgga tatcatggtg ttcttaaaga agaaaatttt ctaggtcaga aattctttgt
     1741 cgacatggaa ctttatattg attcaagaga agcaggtctt agtgacgata taaataaatc
     1801 tgtaagctat gccgaggttt acaatgttgt taaagatatt actgaaaata aacaatttaa
     1861 tcttttggag gctttagcgg aaaatattgc tgaagaagtc ttgaataaat ttatacttat
     1921 aaatggagtt agggtcagag ttagaaagcc tgaggctcct gtaaatggta tttatgacta
     1981 ttttggtgtt gagataagga gagcaagaga tgaataaggc ttatttggga ataggtacta
     2041 acatgggtga tagatttgat aatttatcaa gagcatgtga acttttgaaa aatagtgatt
     2101 ctatatataa agtaaaagaa tcaagcttat acgaaacaaa accatgggga tatacagaac
     2161 aagcagattt tctaaatatg tgtgtagaaa tagaaacaga gtttgaacct tatgagttgt
     2221 tagaatattg tcaagaaata gagagagaac tacacagaga gagaatagtt cattggggac
     2281 caagaactat agatgtagac gtactatttt ttaatgatgt agtttccact gatgaaagac
     2341 taacaatacc tcatccaagg attcaagata gagctttcgt tttgatacca cttatggatt
     2401 taaatgaaaa acttataata aatgaaaaaa caataaaaga acatttaaac ctcctatctg
     2461 ctgaagaaag agaagaagta aaggagcttg taggttatga aagaaaacct atttaataat
     2521 aattcaaaga tagagataaa tacaaataac aaaaagataa ctgtaaaaaa agataaactt
     2581 ataatagctg gaccatgtgc aatagagagt tatgagcaac ttttggagac tgctaaattt
     2641 gtaaaaagtc agggagcaaa tattttaaga ggtggagcat acaagccaag aacatctcca
     2701 aattcatttc aaggattaaa aaaagaaggt cttgaaatat taaaagcggt taaagacgaa
     2761 gttggtatgg ctgttattac agaacttatg gatgtaagag atatggacga actgtatagt
     2821 ataagtgata taattcaaat tgggtcaaga aatatgcaaa actttactct tttaagtgaa
     2881 gtgggaaaac aaaataaacc tgttatgtta aaaagaggaa tagcatccac aattactgaa
     2941 tggataggtg cttctgaata tatagcaata gaaggaaata gtaatattat aatgtgtgag
     3001 agaggtataa gaacgtataa tgattatact agaaatacat tagatttagc tgcggtaccc
     3061 attattcaaa aggaaacagg tcttcctgtt gtggtagacc caagccatgc cactggagtt
     3121 agatatttgg taaaacctat gtcactggca tcttttgcat gtggagcaga tggaataatg
     3181 gtagaagtac atcctgaccc agaaaatgct ttgtcagatg gtgctcaatc tctttgcttt
     3241 aatgaatttg aagatttaat gaaatcaatt aataattatt aaaagaggca agagcctctt
     3301 ttttattcgt aatcgttgct gaggtaaatt tataagttat aatttgatag ttagattatt
     3361 taatataaat aaagatttaa tattgataga attaatataa aaaataaaaa ttttttgatt
     3421 aataagaagg atatcaataa aaaaagtcga attgtgtata ttgagtgatt gacaaattta
     3481 gaatatagtg tagaatagaa atgttaacac aaaaaattac accagaaggt gataatatga
     3541 atgatataaa agacattatt gaagaaataa aagctagatg cgatatagca agtataatat
     3601 ctgaatatat gaatataaaa caatctggag ctaattacaa aggattatgt ccatttcatg
     3661 gcgaaaagac accttctttt tatataaata catcaaaaca gatttataag tgttttggtt
     3721 gtggtgaagg tggagacgta ataaattttg tcatgaaaat ggaaaatttg gattttatgg
     3781 atgctgtaaa aattttagca aataaatgcg gtatagaaat taatactaac atgaatgaag
     3841 aaactaggat taaaatagaa aaatctaaga aatttcaaga tattcatacg gaagcagcta
     3901 gattttatct ttcaaactta ttgggaagca aaaatcttgg gtatgaatat ttaagaataa
     3961 gaggactaga tgataaaatt attaagaaat ttggtttagg attttctttg gattcatgga
     4021 attcacttat gaatactcta atcagtaaag gatataaaaa acaagactta ttggagtgcg
     4081 gtctaattgc aaaaaataga gatggaacta attgctatga taaatttaga aatagagtta
     4141 tgtttcctat attcgattat agaggaaata taataggatt tgggggaaga gtattagacg
     4201 attctttgcc aaaatactta aattctccag acacattaat ttttaataaa aaacagaact
     4261 tatacggatt aaattttgct agaaaaaatt tagaaagtaa aactatagtc ttagttgaag
     4321 gatatatgga tttaatttca ctttatcagt atggtataaa aaatgtagta gcaacccttg
     4381 gaacagcctt gactgaacaa caaggcattt taataaagag atatgcggac actgctatta
     4441 tatcttatga ttctgatgaa gcaggaataa aagcgacttt aagagctata gatatactta
     4501 ctaaattagg tatcaatgta aaagtgttag acttaaaaga tgctaaagac ccagatgaat
     4561 ttgtaagaaa atacggactt agtgattata aaaaagctat ggatgtttct actcactaca
     4621 taaaatataa aatagatcat cttaaaaaag aatttaacat tcaaaaagat gaagaacgag
     4681 tgaagttcgc aaaagaagct tctaaaatta taaaacaatt gacaagtcct gttgaaattg
     4741 atttttatac aaagtattta agcaatcaaa tagatattaa tgtcgagtct ataaagagag
     4801 aagtttatgg taaaaattac aataagccat ataataacaa aaatcaaaag aaaatagaag
     4861 aaaaagtaat tgaaaaagtc gaagtcagac aagacggaaa acaattagtt gaagaaactc
     4921 taataaaaat aatgttagaa gacaaaaaaa ttagagaaat agcgttactt aaagtagaag
     4981 aaagtgattt tttattgaaa gaaagtaaag aaattttaaa ttacatgatt aaaaatcaag
     5041 aattggacaa aataactatt gacaaattga aaagtttaaa catatccgaa gaatacttaa
     5101 aagaattaaa ttcaatttct ttgaatagta taaacttaga gaacacaaaa gaaatagaag
     5161 gaataataac aaatatcaga aaaaactcat tagaagagca aattaatagc ttgttgagag
     5221 aacaacaaga attagaaaat aataatgata tgaaagaggt agatggtaga gttatggaaa
     5281 ttgctttaaa aatagttgag ataaataaaa ttttaaagag cttgtagaga aaggaggttt
     5341 gagtgtggaa aataaatcga ataaaaaaga gttaaaaaag gttactgcta agacattaat
     5401 agaaaaagga aaaaagcaag gttctctgac acttgcagag ataatggaag ctttttcgga
     5461 gactgaactt gataaggatc aagtagaaaa tctttatgag actttaggaa atttgggaat
     5521 agaaataaca gaaacaaaaa actataaagc tgatatagat ttttcggttg ctgatgacga
     5581 tttaagtata ggccacttag atgaagatgc agaggcaatt tcacatgatg actcttctgc
     5641 aatagaaata gaaactgtgg atttatcttt accaaaaggg ataagtatag atgaccctgt
     5701 aagaatgtac ttaaaagaaa taggaaaaat tcctctactt aaaccacatg aagaagtaga
     5761 atttgctaga aggatgcacg aaggtgatga gatagcaaaa caaaggttag ttgaagcaaa
     5821 cttaagacta gttgtaagta tagcaaaaag atatgtagga agaggtatgc ttttcttgga
     5881 tttaatacaa gagggaaatt taggtcttat aaaagcagtc gaaaaatttg actatacaaa
     5941 aggatataag tttagtactt atgcaacatg gtggataaga caagctataa cacgtgctat
     6001 tgcagaccaa gctagaacta taagaatacc agttcatatg gtagagacta taaataagct
     6061 aataagagta tcaagacaac tacttcaaga acttggaaga gatccaaaac cagaagaaat
     6121 tgcaaaagaa atggaaatga cagaagataa agtaagagaa ataatgaaaa tagctcaaga
     6181 ccctgtgtct ttagaaacac caataggaga agaagaagat agccatttag gtgactttat
     6241 tccagatgat gatgctccag ccccagcaga ggcagcagca tattctttat taaaagaaca
     6301 aatagaagat gtacttggtt cattaaatga tagagaacaa aaagtattaa agcttagatt
     6361 tggtcttgag gatggtagag ccagaactct tgaggaagtt ggaaaagagt ttgatgtaac
     6421 tagagagaga ataagacaga tagaagcaaa agcactaaga aaactaagac atccaagtag
     6481 atcaaaaaaa cttagagatt atttagacta aaaagataca tcattgatgt ttttgtgata
     6541 aatgccatga gataagtgat atctcatggc tattttgtag taaagattta attttatttt
     6601 tgtagttatt tcagtctaat atctataact tggattaaat cctataaata aagggaattg
     6661 aaaggagaat tatatttgaa attaacagat agattattga aaatagcatc attagcgtca
     6721 gatggtaaaa aaatagctga tatagggacg gaccacggat acataccagt atatttgtta
     6781 aaagagggaa aagtaccatt tgcagtgcta gcggatgtta ataaaggacc tcttgacaat
     6841 gcacataaag aagtcataca gaacaatctt ttagataaag ttgatttacg attgggttct
     6901 ggtatagaga tacttgaaat aggggaagta gaagaagtga taattgctgg tatgggtgga
     6961 atcttgataa gtgagcttct cgaagctaaa aaagaagtag cacataatgt agaaaaatta
     7021 atattacagc caatgcaagc acaagaagaa cttagatatt accttttaaa caacggatat
     7081 gaaattttag aagaagtttt agtgagagaa gattttagaa tatatgagat aattgttgcc
     7141 aaatatacag gtaaaaatac tataatagaa gatgagatat attatgaggt tggaataaag
     7201 cttttagaaa acaaagattc tctttttaat gattttatag aaaaaaagat aaagacatat
     7261 agttctatag ttaataaatt agaagggaaa aatggggaag ctatagataa gaagagaaag
     7321 gaaagcgaag ttgcaataaa aaaacttgaa aatttaataa aataaatagg aggtatatat
     7381 gctattaaag agtctaacta gaaaaattga aaaaaagtat cctctaaact tagcagagga
     7441 ttgggataat gtaggtctta tagttggaga ttttgatatg gatgtaaaaa aggtattggt
     7501 atctttagaa gctaatgaag atgttattga tgaagctata tccaaaaata tagatttaat
     7561 tgtgacacat catcctttta tatttgggaa aattaataaa attaatagtg gagatttaaa
     7621 aggcagactt atccaaaaac ttattaaaaa tgatatatca ctttattcta tgcatacgaa
     7681 ttttgatata gcatttgatg gtctaaatga ctattttatg gaaattatgg aatttggtaa
     7741 ttcaaaggtt ttagatataa ctaaaagcga aaatttgtat aaattagcag tatacgttcc
     7801 acataactat tcagatgaac ttagaaaggt tttaagtaat tcaggagcag gtcatatagg
     7861 gaattatagt gattgtacgt tttcaacaga aggtgaagga caatttaagc cattagaagg
     7921 ctctaatccg tttcttggaa gtgtaaatga tatagaaact gtaaatgaag ttaaaattga
     7981 aactgtagta ccacagaagc ttttaggtgg ggtaataagt tctatgttag atgcacatcc
     8041 ttatgaagaa gttgcatatg atttgtacaa gttagaaaat aaaggtgaga tatttggact
     8101 aggtagaata agtaaacttg ataaaagtat gacattagaa agtctttcaa ggaaaataaa
     8161 agagaaatta aatatgaaac acataagagt agttggaaac cttagtacag acataacgaa
     8221 agtagccgtt gtaacaggag ctggttcaga atttgtaaaa aaagctaaga gacaaggtgc
     8281 agaagtacta attacgggtg atgtaaagta tcatgaggca caagatgcac ttgatattgg
     8341 aatgtgtata gtggattgtg gtcattttga tacagaagat atattcaaaa atgttatgaa
     8401 gagattttta gatgaattta gtgaaattga agtaataaag agtaatgtat atttgaatcc
     8461 atttagtact atttagcagt gtttgataat aaaaatacta tataaaaaat aaatttgagt
     8521 ttaattaggg ggataatgat gaaagtagca gtaatttttc atagtgtttg tgggagtaca
     8581 tatttattag ctagagaata taaagaagcg cttgaagaaa tgaatatcga ggtagatata
     8641 tttagagtaa gtgacgaagt tgcaaaaaca cttcctcaat attatttgat aaattccaaa
     8701 gaatacaaag acgaatttga aagtataaat gtaattaaat ctggaaaaga aatattagat
     8761 tatgatgcta tatttatggg ttcaccaaca tattatggaa atgtatctgg accaatgaag
     8821 atgtttatgg atagtttttc tgatatttgg gtaggtgctc ctttgagtgg taaaatattt
     8881 ggttgttttg ctacagcagg ttcacaacat ggaggaggag aattagcttt gcaagctatg
     8941 aatatatttg cccaacatat gggaatgact cttttgtcag ttccatgttc agtaagagga
     9001 ggttatccag cgtatggaat acttcatata gcaggagata attctgatat aagaccaaat
     9061 gatgatgcca agatgggtat aagagattat ctaaaaagac ttaacatata agaatatcat
     9121 ataaaaatac ctaaaagcga ctgtatatac ttttaggtat ttttacttaa ttataaacgt
     9181 gaagctttac cagaaatatg gtcaatttct attttaacaa cagttgttaa gttcatatcc
     9241 ttctctatgt acttcatgcc ttcttcaaag aatcctttag aatatttttt tattatttct
     9301 acaagagcat ttttcttttc ttcattttct acagtgcaag cttttccaaa aactatagca
     9361 ctagaataag tagtactaaa tttatctgga ataacacttt cattagcaac tactaaaaaa
     9421 gaaactttgt tgtttttcga tatattatct aacttatgcc catttcttgc acaatgaaag
     9481 taaatagaat cattaaagta tacataattt acagcaactc cataaggata accattttca
     9541 gatatagtgg aaaaagtacc aaattcacca ttttttaata cttcaacagt atcttctttt
     9601 gtcatttctc tttttttcaa tctcatttct ttaaacataa tatcatcctt tctaattttg
     9661 atgcttaaat taaattttag aacatttata aagattatgc tatgtttttt acaaatttta
     9721 agaaaaaaat atagtttaag ataaaaggta gtgaaagaga agctatttgg aggtttattt
     9781 atgatattat tgttagagta gtataaacaa tactaataaa tttaatagct ggtttcttgt
     9841 tatttaaggt agtaagcatg tctataagaa ggggaagtta tcgagggtag aagttgttat
     9901 ttataacgat attataaaga aaatattatt atataaaata ataaatagta aagaggtatc
     9961 aagttgaata taatacttaa tacctcttca aaattaagtt ataaaagtgt tgtgggaatt
    10021 gtcctataca tggtaaggag ataataaaga atgtatcttt aaagtctcaa ctaaacagca
    10081 aaatttatcc aacaaacata caataaatgc ttctttgtat ctaggtggct taacagtaag
    10141 tggccacatg tgttttaaaa ttatgtccgt ttctttttca tttatttgaa aaaataaagt
    10201 tgcatttttt aatgcttctc tagggtgagt aaatccatgt aatccttttc tatcaccttt
    10261 gtaatgccaa tcataaagaa aaaagtcgtg tagtaaagct cctctaataa cactttgaat
    10321 atcaacactt aagtgcaatt ttttacataa tagataacta taatatgcta ctgacaaact
    10381 atgttcaaga catgaaacat tgccatgatg aggaaatgtt ctcataagtt gaactttttc
    10441 agaagaaagt atctcagaag cacattcatt aaaatatttg atttcagctt ttgacaacat
    10501 tatgaaaatc tccattctta gtaatatata aatactatac ttcaacgata atatgaaata
    10561 tttcttatcc tacaaaatat aatacccaat aatacaagta agataccttt tatggtattt
    10621 taaagtaaga gagattttat taggctaaac aatgaaaaaa ttaaaagtaa agctatagca
    10681 atatatagta tttttccaag aggattgcca aaatttatag tccatccaat accaaacctt
    10741 ttattaacca ttaatgatgg gtcatttgga ttattataca tgatgcctgc tatccaatac
    10801 ttctcatctt cttctggaga ataagatgag ttgaatttta aatttgggga attaatatat
    10861 gttgctatta aataaattat aactaaaagc gttgtaatat tcatagcagt tgatagtttt
    10921 gtttttattg ataaaagagt agtaaaaaac tgtgttgtca ttataatcat caatattaaa
    10981 aacaaataac caattttatt taaatattta atgttttcaa ctctactttc ttcaattcta
    11041 tttgtatcta tcttaatcct tgactttatg gatccaatgg aagttatata taataaaacc
    11101 attaaacaaa agtcagtacc gattaattta aatacattaa gaaatgattt atctataaaa
    11161 acatcaggag acccatttat gttccaatga gtggcaattg agtctggcaa ttgattataa
    11221 ttaaatgcta taaaaatact cattcccaaa gtaaatagta tcggtattaa atagattatt
    11281 ttaaattttt
//
LOCUS       DAKBNN010000129        11210 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000129 DAKBNN010000000
VERSION     DAKBNN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.130"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            281..1027
                     /locus_tag="OUB02_002698"
     CDS             281..1027
                     /locus_tag="OUB02_002698"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCT8886442.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            1057..1992
                     /gene="rsmH"
                     /locus_tag="OUB02_002699"
     CDS             1057..1992
                     /gene="rsmH"
                     /locus_tag="OUB02_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCT8886443.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            2474..2821
                     /locus_tag="OUB02_002700"
     CDS             2474..2821
                     /locus_tag="OUB02_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886444.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            2833..4812
                     /locus_tag="OUB02_002701"
     CDS             2833..4812
                     /locus_tag="OUB02_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HCT8886445.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            4979..6352
                     /locus_tag="OUB02_002702"
     CDS             4979..6352
                     /locus_tag="OUB02_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCT8886446.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHAYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            6393..7361
                     /locus_tag="OUB02_002703"
     CDS             6393..7361
                     /locus_tag="OUB02_002703"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCT8886447.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            7380..8735
                     /locus_tag="OUB02_002704"
     CDS             7380..8735
                     /locus_tag="OUB02_002704"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCT8886448.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            8787..9917
                     /gene="spoVE"
                     /locus_tag="OUB02_002705"
     CDS             8787..9917
                     /gene="spoVE"
                     /locus_tag="OUB02_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HCT8886449.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            9935..11161
                     /gene="murG"
                     /locus_tag="OUB02_002706"
     CDS             9935..11161
                     /gene="murG"
                     /locus_tag="OUB02_002706"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCT8886450.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
BASE COUNT         4443 a         1205 c         2170 g         3392 t
ORIGIN      
        1 gagctataga taagaattag aagaggttga tgtatatatg catcaatctt tttgattaaa
       61 aaatatctat gtacacttgt aactttagtc atgagagatt cagtttaaaa aattttatag
      121 taattagata taatatagtg tagggagttg tagaataaat tttaatattt tttgtagaat
      181 taacattaat gtgttatcat aatttagtat aattacaatt aattgtaatt atagcatatt
      241 gctttggaaa tattttcaat taatacaaag aggtgacgtt atggatagag tagcatttac
      301 actgtttggc atagacatca tgtggtatgg aatacttatg gcatgtggaa tgattttagg
      361 aactttaata gcaataaaag aagcaaaaag agtgggaatt aaagaagatg atgtattaaa
      421 tatagctata atagcaattc cagttggact aatctgtgca agaatctatt atgttgtatt
      481 taattggagt tactatgctc aaaatatgtc tcaaattttt aattttagag gtggtggact
      541 tgctatacat ggagggttaa ttggtggaat attagctggt tatatataca ctaagattaa
      601 aaatattaac ttcttaaaaa tggctgatac agttatactt ggaatgcctt tagcacaagc
      661 tattggaaga tggggaaatt ttattaatgg agaagcacat ggaggtgcta ccaatttacc
      721 ttggggaata atggttgatg gagtaaaggt acatccaact tttttatatg agtctatatg
      781 ggattttgga atatttatag ttttattact atttagaaaa aataaaaaat atgaaggtca
      841 agttatagtt acttatataa ctttatattc aataggaaga ttttttatag agggattaag
      901 aacagatagt ttaatgttag gaccacttag aatggcacaa gttataagtc taattggagt
      961 tattggtggc ataattgcac atgtttattt atcaaagaaa aataaaaata atattagtga
     1021 agaatagttt aaaaagtgaa tttaagggag gtaactatgg aatttcatca tgtatcagtt
     1081 cttttaaatg agtgtataga gaatttaaat ataaagccag atggcgtgta tgttgactgt
     1141 actatgggag gagcaggaca ttctaaagaa atagtaaaaa aactgtcaga taagggatta
     1201 tttatagggt ttgaccaaga taaaaatgct ataagcacag caaaagagag attgagtgaa
     1261 tatgaaagta gagtgaagtt tgttcacagt aattttgaaa atataaaaga ggaattagaa
     1321 aaaataggag tttacaaaat tgatggtgta ttagctgatt taggagtttc atctcatcaa
     1381 cttgatgaag ctgatagagg attttcttat atgcaagatg caccacttga tatgagaatg
     1441 gatgttaggt gtgagttttc tgcatatgat gttgtaaata cttatacaga agatgaatta
     1501 actaagataa taaaagatta tggagaagat aactgggcta agcgtatagc taaatttata
     1561 gttgaagaaa gagcaaacaa accaatagaa actacaggag aattagtaga tgtaattaaa
     1621 aaagctatcc ctaagaaagc tagaatagat ggacctcacc cagctaaaag aacttttcag
     1681 gcaatacgaa tagaagtaaa taatgaactt ggagtaatta caaagatgat aaatgatgct
     1741 tcttctataa tgaatgaagg tggaagaata tgtataataa cattccattc attagaagat
     1801 agaatagtga aaaatgcatt taagcattta gcatcagatt gtatatgtcc acagcattta
     1861 cctatatgcc aatgtgataa agaatctgag gtgaaaataa taactagaaa acctatatta
     1921 cctagtgagg aagaaataga agtaaatcca cgttcaagaa gtgctaagct aagagtagca
     1981 gaaaaaatat aagtagttat aaggctaaag aataatctta tatataaaat aagattatta
     2041 taattaatta aaaagtatta tatattttgt tgcactggta aagcaattaa aaaacaatta
     2101 acactataag aaatgttctt tgttattgag atatataatg aaaatttaga tataaaaata
     2161 tagatataga tggtattaag taatataatg gagaacttag gaatcagaga gtagttagtt
     2221 acatattata tttatggaag aaattatcta aaaatactag aagtgataaa aataaataga
     2281 aattaatatc taacctatag attcatataa aagtataagt gaatttatag gtttttgttg
     2341 tgtatagaaa aaaatacaat tagattgtgt aatagtgtat taatttagcg atgaaatttt
     2401 agaggaagaa atatgattaa taacaaatat agtatagagg aaaatttaaa ctatatacta
     2461 tcggggtgag aatttgaaaa aaaattataa aatacataat ctaaatgaat ataaaaataa
     2521 aaaaaagaat aattataaaa agagaaataa gaaaaaaata ggagctaaat gcattttact
     2581 gattgcaact ttttcagtaa taatattgaa tctatgtggt tattcaaaaa tctcggaaat
     2641 gaagtacgat atctattatt tacaaaaaga tttaagaaaa aaagagatag tattagagga
     2701 actaaaggca aaagaaactg aaaaaacatc aataaaagaa attgagctaa aggctaagga
     2761 attgctcaat atggactatc caaaagatgg acaaataaag tatatagatg tggataacta
     2821 gggggaatag atttgagaaa agtaaagagg ataagtaaga aaaggctggt actcgttctt
     2881 attctagcat gtgcattgtt tttttgcctt gttattagga ctgggtatct acaacttatg
     2941 aagggaaatt ggttgagtac aaaagcacta gaacaacaaa caagagatat accaatagaa
     3001 cccaaaagag gaactatata tgatagaaat atgaaagagt tagcagtaag tgtaactaag
     3061 tatactgttt ggtgtaaacc tgtggaagta gaagataaaa aagaagcagc cgaaaaggta
     3121 gctgagattt tggatgaaga ttataaagat atttacgctt taataagcaa aaaaaatatg
     3181 gctcttgtaa aagtaaaaag atggatagat gatgataagg caagtcaaat aagagatgct
     3241 aaattaagtg gaatatgggt ggcagaagat aatcaaagat attacccata tgggaatttt
     3301 gcaccatatg tgttaggaca tacttcttca gatgcaacag gtatatctgg tgtagaaatg
     3361 caatatgata aaaagttaaa aggaaagcct ggaaaactta tagtaagtac tgatgcttca
     3421 ggaagagaaa taccacaagg aatggaaaaa tattatgagc ctgtacaagg taatggtctt
     3481 gtgttgtcta tagatgaagt aattcaacac tatactgaaa aagcagtaca gaaagcttat
     3541 gagttaaata atgcaaaaaa agttactgca atagctatga accctaaaac aggtgatata
     3601 cttgctttag catcaaaacc agattatgac cctaatgatt caagaacgcc aatatatcca
     3661 tattatcaag aagaacttga aaaatataat gataaagata aaataaaagg ctattatcaa
     3721 atgtggagaa atccagcagt aagtgataca tatgaaccag gttctacatt taagcttatt
     3781 acatcttcca gtgctcttga agaaggagta attaaagatg gagaaaaatt cacatgtaca
     3841 ggtagtgtaa ctgttggagg aagaaagatt aaatgttgga gacattacag acctcatgga
     3901 acacaagaat ttaaacaagc agtacaaaac tcttgcaacc cagtatttgt tgagttagga
     3961 agcagattag gtgttggaaa aatgtatgat tatatagaat catttggtct tatggataag
     4021 acaggtatag atttaccagg agaagctaag ggaatactgt ataacgaaaa aaatgtagga
     4081 ccagttgaat tggctacaat atcatttggt caatctatat cagtaactcc aatacaactt
     4141 ataacagcta tatcatcaat agctaatggt ggagatttaa tgcaaccaag agtggtaaaa
     4201 tcttatactg acaataaagg aaatataact gaaacagtaa aaccaaaaaa agtaagaagt
     4261 gttatatcta aagaaacatc taaaaaaatg ttagaaattg cagaatcagt tgtaactgaa
     4321 ggtggaggaa agatagcgta tataccaggt tatagacttg gtggtaaaac aggtacagct
     4381 caaaaagtta tagatggtaa gtatgctcca ggaaagtata tatgttcatt tgttggaatc
     4441 gcaccttgtg atgacccaca aatagtagtt cttgctattg ttgacgaacc aacaggagta
     4501 agtgcatttg gtagtactac agcaggacca atagtgaaag aaataatgaa tgactcttta
     4561 aaatatttgg gagttaaacc agtatataaa gaagaagaaa aagctgagta tgaaaagaag
     4621 caagttaaag tgccagatgt aagaaatcta aaaataggag atgctgtaaa ggcattggag
     4681 gatgcgaagt taaaaccaga cttagatgca gatatagaac ttccagaaga tacaaaggta
     4741 aaggatatat tcccaaaacc tggagtaaaa gttaatgaag actcaagtat aaccctatat
     4801 tttgaaaact aaaaagtaac agataattaa catataccca taaacatctt caaaaaaaca
     4861 gaaatatagt atataattat aaagttaaga gttatcgtag gcaatgtata tataacggta
     4921 tacattgcct tttgttgcta agagaatata gctaaggttt taaaggagga gtttaataat
     4981 ggagtgctta acaataaaag agttagtact tgcaactaat gggaagttaa tatatggaga
     5041 ttataatgac tgtgtaagtg atatagttat agatagcagg gaggcaaatg cccaaaatgc
     5101 atttgtagcc atagttggag aaaatttaga tgggcataca tttatgaagt ctgcatatga
     5161 tagtggatgt aaaactttta taaaaaatga aagtaatgga ataaaattag aaagttcgga
     5221 tataaattta atagaggtaa aagatacatc tttggctcta ggagatattt caaaatacta
     5281 taaagaaaaa tttgatattc catttatagg tgttacaggt agtgtgggaa aaactactac
     5341 tagagatatg atttactctg ccatatctgc aaagttaaat atactaaaaa atgaaaaaaa
     5401 cttaaataat cattttggag ttccattgac tttatttaat ctaaataaag aacatgagtg
     5461 tgctgtaata gagatgggta tgtctggatt caatgaaata aaatatttag tggatatagt
     5521 aaatcctaaa attgcagtca tatcgaatat aggattatca catgtagaaa aactaggttc
     5581 acaagagggt attttgaaag caaagatgga gattacttca aactttgatg aaacaaatac
     5641 tttaattgta aatggagatg ataagttttt atctacatta aaagaaaaag aacatgcata
     5701 taagttaaaa acatttggct ttaataaaaa caatgatgtt tattgtgaaa gttatactat
     5761 ggaagaggat agtttaacat ttatatgtgt aataaatggg aaaaaagagg aaatatttat
     5821 accaacaata ggagaacata atatatataa tgcaatgtct gctatattag ttggtttgtg
     5881 tctaaatatt tctttagatt atataaaaaa aggtttaaaa aattttaaag ggacaaaaat
     5941 gagattagac ataataaaaa atgaaaaatt aacaataatt aatgattcat ataatgctag
     6001 tccagattct atggatgctg ctttaaagat acttggaaga tataaaggta gaagggtagc
     6061 gatacttgga gatatactag aaatgggtga aatttcagag tatggtcata gattagttgg
     6121 aaaatcatct atgaataata ctgatattat aataactata ggtgaaaatt cagtgtttat
     6181 tggagaagaa gcaaaacaat taggattcaa ttcagccaat atatatcact ttgagaatag
     6241 agaagatgtg tttaacaagt taaatgaatt agtaaaaaca ggagacacta ttttagtaaa
     6301 aggctcaaga ggcatgagat tagaaaaaat agtcgaatat ctaaataaat aagcttaaac
     6361 taatgagtta attgacaagg gagaggagtt ttatgatgtt aggaataaca gagcttacgt
     6421 acaccgcatt gattgcgttt ttaatagtaa ttataatagg accaatattt ataccaatgc
     6481 ttagaaaatt taaatttggt cagacagtta gagatgatgg accccaaaca catctagcta
     6541 aaaacggaac acctactatg ggtggaatta taatgattgt tgcaattcta ataactggac
     6601 ttacaagagt aaaagttagt catgatatgg cagtaggtct tatatgtatt gcaggttttg
     6661 gttttattgg ctttttagat gattttataa aaataaagct aaaaagatca cttggactaa
     6721 aagcatatca aaaaataatc cttcaagttg cattatcttt ttatgttgct ttttatcagt
     6781 atacatcatc atcaagtgct tcacaactta tgataccatt tacagatttt gtgataaatg
     6841 ttggaatatt atatatacca ataatgatgt ttataatcgt agcaatagta aatgctgtaa
     6901 acttgactga tggattagat ggattagcat caggagtaac attaatagtt tctgtattct
     6961 ttatgctatt tgcaagttct attgctggaa atacagaagt cgcagtttta gcagcagcaa
     7021 ctgttggagc atgtttaggt ttcttaggat ttaattcata tccggctaga gtgtttatgg
     7081 gcgacacagg ttcaatggca ttaggaggtg cagttgtagc attttcagtt ctaactaact
     7141 cagtgcttat aatacctata attggaggaa tttattttgc ggaggctcta tcagttttga
     7201 ttcaagttgg atattttaag gcaacaagaa agagattctt taagatggct ccaatacacc
     7261 accactttga acagtgtggt tggccagaaa caagagttgt atttattttt tggattataa
     7321 cagttgtttt agcttggata agtataatag cagtatttta gggctagggg gaatgaaaaa
     7381 tggacttaaa aggaaaaaaa gttttattag taggacttgc aaaaactggt atatctacaa
     7441 ttaaacattt agataaattg ggtgcaagta taatcgtaaa tgacataaaa gatgaaaata
     7501 aattgagaaa tatacttgat gaattgaaat ctataaatga cataaaatac atattaggac
     7561 atcacccaga agatgtagat gatatagata tggttgtagt gtctccaggt gtaccactag
     7621 acctaccatt catattgaaa ttaaaaaatt caggcaagta tataattggt gaggtagaat
     7681 tagcttttaa actatcgaat aatccaattt ttatagggat tacaggtaca aatggaaaga
     7741 caactacaac tagtttggta ggagaaattt tttcaagagc aaaacgagat acatatgtag
     7801 ttggtaatat aggaaatcct gtaatagata ctatagaaac ttcaagtgaa gagtctgtgt
     7861 tagtaactga actaagcagt tttcagcttg agagtataga tgaatttaga cctaaagtaa
     7921 gcgcaatatt aaatattaca gaggaccatt taaatagaca ccatactatg gaaaaataca
     7981 tagaagcaaa ggcaaatata tttatgaacc aaactgttga ggatttctgt gtattaaact
     8041 atgatgatga gatagtaaaa tctttagctg ataaatgcaa tgccaaagta atttactttt
     8101 caagaactaa aaaagtaaat ggaggagtat atttagaaaa taatgacata ataatagata
     8161 tagatgataa aatcaaattt ctaaataaag atgatgtaag tttacctgga ggtcataact
     8221 tagaaaattg tatggcagct atagcaatag catatgtctg caaaattgat ttagaagtta
     8281 taaaggacgt acttatgaca tttaaaggag tggaacacag acaagagttt gtaagaaatt
     8341 tagacaatgt aatatatgta aatgattcaa agggaactaa tccagattcc actataaaag
     8401 ctatacagtc ttatgataga cctataatac ttatagcagg ggggatggat aagggaagta
     8461 attttgatga acttcttgaa actgcaaagt catatgttaa atcattggtc ttattaggag
     8521 aaactgcatc aaatatagaa aattgtgcta aaaataaagg atttaatgac atacatatag
     8581 taaaagatat ggaagaagca gttaagactt cttatgaaat ttcaaaaagt ggagatatag
     8641 tgcttctttc accagcttgt gcaagttggg atatgtatga aagttttgaa gtaagaggaa
     8701 aagacttcaa agataatgta aacaatttaa aataatctct tacctctaaa cttacttaaa
     8761 tggaagaatg gaggtagatt agttggatgc ctaaagaaaa tttgaaaaaa caaattgaca
     8821 taaggatgaa gactgaattt gatggagtag tgttttacac tactatgctt ttagtatttg
     8881 taggaatagt tatggtgttt agtgcaagtt ttatacaatc ttcatttaaa cacaatgatg
     8941 cgtactattt tctaaaaaag aatgtaatat atgctgtact tggatttata gttatgatta
     9001 ttacatcaag aatagattat agtttttgga agaaaaatgc tacagcaatt ggagctatag
     9061 cagtagtatt attattgtta gttttgaccc ctttagggat agaagcaaac ggagcaaaaa
     9121 gatggttggg aataggagct ttaacatttc aacctgcaga aatcgcaaag tttgctacaa
     9181 taatattaac tgcaaaatta attgaaaaaa attatgataa aataaaatct ttgacaaaag
     9241 gtgttgtacc acttctcgta gttccaggga tattttttgc gttgataata cttcagccaa
     9301 atttgtcaac agcaggtact gtaattctag ttacttttgt aatgatattt gttgctggaa
     9361 tggatatgaa aattgtattt gctatgatag ggagtggggc tgctttattt gctgcattag
     9421 taattgctga accctataga cttagtcgtg ttacatcatt tttagaccct tttcaagacc
     9481 ctcttggtaa ggggtatcag gtaatacaag gtctatatgc actaggttca ggtggattgt
     9541 ttggtctagg cttaggaaag agtaaacaaa agtattttta tataccagag ccacaaaatg
     9601 actttatatt tgctataata ggagaagagt taggattgat tggttgtata atagttatta
     9661 tgctatttgt agttttagtc tacagatgtg taagaatagc tttgaagact tctaatgtat
     9721 ttgcttgtat ggttgttata ggtataggtg cacaaattgg aatacaagca gctttaaata
     9781 tagcagttgc gacatcatca atgccagcta caggtgtagc actaccattt ataagttatg
     9841 gaggaacttc actcaccatc tttatgggag ctgtaggaat agtgttaaac atctcaaagc
     9901 atgtcaaaat taattaacag gagatgatga tttgatgaaa gttttattat caggcggtgg
     9961 aactggaggg cacgtgtatc cagctatagc tattgcaaac aaaataagag atgagcatcc
    10021 agacgctgag ataatttttg ttgggacgga aaagggaata gagtcagaaa tagttcctaa
    10081 gtatggattt gaattaaaga ctgtaacagt tcaaggtttt aagcgaaaaa tagatttcga
    10141 taatgtaaaa agagttttta agctttttaa aggtttggag caatcaagaa agattgttaa
    10201 gaaatttaaa ccagatatag ttataggaac tggtggatat gttagtggac ctgttttatt
    10261 taatgcatct atggggaaaa taccagccat aatacatgag caaaattctt ttcctggtgt
    10321 aactaataag attttatcta aaacagttac aaaggtactt acaagttttg aagattcgca
    10381 taaaagattt ccagaggcag cagaagataa attagttttt acaggaaatc cagttagaaa
    10441 agaaatttta ttatcaagaa aaaatattgc aagaaagaat ctatcaataa gtgacgaaaa
    10501 aagaatggta ctttgctatg gtggtagtgg aggttcaaga aaaataaatg atgcaatgag
    10561 acttgtaata aagaatatgg taaatgaaga tatagctttt atatttgcta caggcaaaag
    10621 ctattatgat gaatttatgg gaagtattag cgatataaat cttaaaccat accaaaaagt
    10681 tgtgccttac ttagaagata tggcaaatgc acttgcagca agtgatttgg ttataggtag
    10741 cgcaggagca atttcattag ctgagataac ggctcttgga aagccatcta taataatacc
    10801 taaagcttat actgctgaaa atcatcaaga gtataatgcc aagagtatag aaaaacaagg
    10861 ggcaggaatt gctattttag aaaaaaatct aactccagag agcctaaata cagcagtatt
    10921 taaactttta ggagatagag aacttttggt tgatatggca aatgcaagta aaactatagg
    10981 taagcctgaa gccatagacc ttatatatga tgagataatg aaagtataca attcaacaca
    11041 aaagtcaact tctaaaaaaa caaaaaaaga aaaagttatt aaggaagtaa aagaaataaa
    11101 aaaagaaacg actccaagta tagaaggtca agctaaggta ataggaataa agaaaagata
    11161 agtaaatatt tacccctaat ttataatata ttagattttc taaattatta
//
LOCUS       DAKBNN010000130        11103 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000130 DAKBNN010000000
VERSION     DAKBNN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.131"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            351..1673
                     /gene="dnaA"
                     /locus_tag="OUB02_002707"
     CDS             351..1673
                     /gene="dnaA"
                     /locus_tag="OUB02_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCT8886451.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1915..3021
                     /locus_tag="OUB02_002708"
     CDS             1915..3021
                     /locus_tag="OUB02_002708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCT8886452.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3158..3364
                     /gene="yaaA"
                     /locus_tag="OUB02_002709"
     CDS             3158..3364
                     /gene="yaaA"
                     /locus_tag="OUB02_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCT8886453.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3382..4497
                     /gene="recF"
                     /locus_tag="OUB02_002710"
     CDS             3382..4497
                     /gene="recF"
                     /locus_tag="OUB02_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCT8886454.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4498..6399
                     /gene="gyrB"
                     /locus_tag="OUB02_002711"
     CDS             4498..6399
                     /gene="gyrB"
                     /locus_tag="OUB02_002711"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCT8886455.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6419..8845
                     /gene="gyrA"
                     /locus_tag="OUB02_002712"
     CDS             6419..8845
                     /gene="gyrA"
                     /locus_tag="OUB02_002712"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCT8886456.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8914..9210
                     /locus_tag="OUB02_002713"
     CDS             8914..9210
                     /locus_tag="OUB02_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCT8886457.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9210..9470
                     /locus_tag="OUB02_002714"
     CDS             9210..9470
                     /locus_tag="OUB02_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCT8886458.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9482..9799
                     /locus_tag="OUB02_002715"
     CDS             9482..9799
                     /locus_tag="OUB02_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HCT8886459.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9824..10210
                     /locus_tag="OUB02_002716"
     CDS             9824..10210
                     /locus_tag="OUB02_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCT8886460.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFYIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10210..10983
                     /locus_tag="OUB02_002717"
     CDS             10210..10983
                     /locus_tag="OUB02_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HCT8886461.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4514 a         1152 c         2018 g         3419 t
ORIGIN      
        1 cattgaagtt tgacatatat tttgatatta taaatatact tgttgataat gtgcataaat
       61 gttatcaact tattagaaat tattcacagc ttgtggatac ttttgtgtat aacttttaag
      121 attactgaca atactctagt tataatcacc atttattgta tgtttatgtt atttataact
      181 ttatctacat attgtttata tcttttcata tttttttaat aatttatact attattaatt
      241 tatcaacacc attatccata aatttagtta ttctgtaaaa tttatatata tttttgcttt
      301 tctgttaata actttgtgga aaatattaat tattctgttc ggaggtttat attatggata
      361 tagtttcttt atgggacaaa accctacaat taataaaagg tgacttaact tcagtaagtt
      421 ttaatacctt ttttaaaaac atcgtaccat taaaaataca tcttaatgat ttaatcttac
      481 tggctcctag tgattttaat aaagatatct tagagaatag atatttacat ttaattgaag
      541 atgctattag tcaactgtca ttaaaaaaat ataatattaa atttgtattg agcgaaaaag
      601 aggttgctga tttaaattct gatagcacag atttaaatta tagagttctt taccccaatc
      661 taaatccaaa atacacattt gatacattcg ttattggtaa tagcaataga tttgcacatg
      721 ctgcatgcgt tgcagttgca gaatctccag ctaaagctta taacccactt tttttgtatg
      781 gaggagttgg attaggtaag actcacctaa tgcatgctat aggtcatcat attgttagtc
      841 aaaaaaaaga ctcaaaagta gtttacgttt cttcagaaaa atttactaat gaacttataa
      901 actctataaa agacgataaa aacgaagaat ttaggaataa atatagaaac gtagatgtat
      961 tactaatcga cgatattcaa tttatagcag gaaaagaaag aactcaagaa gagtttttcc
     1021 atacattcaa tactcttcat gaagcaaaca aacaaataat tatatctagt gatagaccgc
     1081 caaaagacat acctacacta gaagataggc taagatcgag atttgaaatg ggacttataa
     1141 ctgatattca agcacctgac tttgagacaa gaattgctat acttagaaaa aaggctcaac
     1201 tagaacgtat agatgtgcca aatgaggtta tgtcatatat agcaaaaaat attaagtcaa
     1261 acataaggga actagaaggt gcattaacta gagtggttgc ttattcttct cttagcaata
     1321 gggtaatatc ttttgatctt gctacagaag ctttaaaaga tattataaca acttctaaaa
     1381 atgaagaaat aaatgttctc agaataaaag aaaaagtatc ttctgtattc aatttaaaga
     1441 tggaagattt taattctaaa aaaagaacac gttcaatagc ttatccaaga cagatagcta
     1501 tgtatttaac gcgcgagctt actgatttat cccttcctaa aataggtgaa gagtttgggg
     1561 gtagagacca tacaacagta attcatgctc atgacaaagt atctaaggat atagaagaaa
     1621 gtgaagaaat taagactaaa atagacaaaa ttatatcaga tttgaaggga taatttatac
     1681 acaattgtat gtttatatct cttgaataac ctgttaatac tttttttgaa caaaaattat
     1741 taacaaatta tcaagtctaa aataacaaag atattaacat cttatacaca tgttaatatc
     1801 tttgtattta taatgttata catacttatt aacattatca acaacaccta ctactattac
     1861 tactatgatt ttaacttatt tttatatata aaaagccaga ggaggttatc cacattgaaa
     1921 ataatttgta atcaaaaaat cctagcaaat agaattggaa ttgctcaaaa agctattaat
     1981 ggaaaaacaa caatagaatt attaaaaggt atacttatat ctacagaaga aggtcaatta
     2041 aaacttactg gatatgatgc tgaaatagga atagaaactt atgtacaagc agaaatcata
     2101 gaaaaaggtg atgttgttgt agatgcaagg ctatttggag atataattag aaaattacca
     2161 gattcctttg ttgagataga aactgattca gaaaataata tttacataaa ttgtgtaaat
     2221 tcaagattta aaattaaagg ttatgcagca aaagaatttc ctaaattacc agaattaaat
     2281 gaagaagatt tatatagcat acctcaagaa atcctaaaaa atatgattaa acaaactgta
     2341 tttgctatct cacaggacca aacaaaacct gttttaatgg gagaactttt agaaattgta
     2401 gatagaaact taaatttagt agcaattgat ggatatagat tagctgtaaa aagttgttcg
     2461 gttgatagtt taactgaaaa tataaaagtc ataattcctg gaaagacact tatagatgta
     2521 aatagtttgc tttctggaga ggataatgtt aaagttggat ttaacgaaaa aaatgctatt
     2581 tttataatta atgatacgaa aattattaca agattgcttg aaggagattt tattgattat
     2641 aaaaaattat tgccaagaga acataacagc agagttaaat taaatactaa ggaactttta
     2701 aatagtatag agagagcgtc tttattgtct caatcagaaa aaaataatct tataaagtta
     2761 tctataagag ataaagtaat ggcaattact tctaatacag aaaaaggaaa tgtgtatgaa
     2821 gaagttgaaa tagatttaga tggagattac ctagatatag ctttcaattc tagatatttt
     2881 atcgaaggtt tgaaaaatat agataatgag gaaatattta tagaatttac tacaaatgta
     2941 aatccttgta taattaaacc aacagatgat gttaattata tttacttact gcttccagta
     3001 agaatatcat caaatatata gcagacatga aggagttgtc ttttgtagaa aatttttcta
     3061 atcttagaca actcctttta taagcatatt tatggtataa tatactttta gtgtatcaat
     3121 aggaatttgt ttaaaatagt gacaaggagg aactattatg actgaaataa ctatagaatc
     3181 agaatatata aagttggatc aatttcttaa attagcagag attgcatcaa caggaggtca
     3241 tgcaaaattt ttaattcaag aaggtttagt gacagtaaat gatgaaatag aattaagaag
     3301 aggaaaaaag ataaaatcag gagatatagt tgaaatagaa ggaactaaaa taaaagtatt
     3361 gtaaaaaaag gagaactttt tgtgaaactt aaaagtttgc aacttgttaa ttttagaaat
     3421 tataaaaagt tgcatctgga atttaatggg aaagtaaatc tacttgttgg taaaaatggt
     3481 caaggaaaga caaatatagt agaatctatt tatatgcttt cttttggaaa atcatttagg
     3541 actaacaaag ataaagaaat ggttagattt aatagtgaaa acttatatat tggaggaagt
     3601 ttttcaaaat ataataaata tagtctaata gagctaatta ttggaaaaga taaaaaaggg
     3661 ataagaataa ataaagttcc tttacaaaag atacaagaac tacttggcaa cttaaatgtt
     3721 gttatatttt ctcctgagga tttaaggttg gttaaagagg gtcctaaaga aagaagggca
     3781 tttatagaca aagagattag tcagattata cctaaatatt ataaatatct taccaattat
     3841 aataaaacac tttctcaaag gagtagggtg cttaaaaata tacatgttga tgaagcttta
     3901 ttagatgtat atgatgatac gttagcaaag tatggaagtt atatttatat tttgcgtaga
     3961 gactttataa aaaagatagc taatatatct gaaaatatgc atatgaattt gactaatgga
     4021 gttgaaagat tatcaattcg atataaaaat caaataaata taactgatga agatactata
     4081 gatacagtat ataataaatt tttagccaag ttgtcatcga atagacctaa tgatatagaa
     4141 tctaagacta ccagatatgg tatacacaaa gatgacttaa atatattcat aaatgattta
     4201 gatgcaagac tttttggttc acaaggtcaa caaagaactg cttcaatatc attaaagtta
     4261 tcagagatag aattaataaa aaatgaagtt gaagaatatc cagtattaat tttagatgat
     4321 gtttttagtg agttagatga agcaagacaa aaacttttag taaataattt aagcaatgtt
     4381 caaatgttta taacaagtgc tgaagtttca cataaaaaaa tatttgatga aaaaaatgtt
     4441 acaatattta atattgaaaa tggcgatgtt attagcatag agaatggagg aaattaaatg
     4501 aaacaagaat acggtgcaag tcagatacaa gtattagagg gattagaagc cgttagaaaa
     4561 agaccaggta tgtatattgg tagtacaagc cctagaggtt tacatcactt agtttatgaa
     4621 gttgtagata atagtataga tgaggcttta caaggatatt gttcagacat atatgtatct
     4681 attaatgaag atggcagtgt tttagtaaaa gataacggaa gaggtatacc tgttgaaata
     4741 catcctaaaa ctggtaaatc aactttagaa acagtattaa ctaatcttca tgcaggagga
     4801 aagtttggag gcggaggata taaagtatct ggaggtcttc atggagttgg tgtttctgta
     4861 gttaatgcat tgtcaaaatg gatggtagca gaggtttatt taaacggaaa gatatataag
     4921 cagacatatg agaaaggatt gccaacatct aagcttgaag tagttggaga atctcaagat
     4981 aaaggaacaa tgattcaatt tatgccagat gagacaatat ttgatgaaat agagtttaaa
     5041 tatgaaacat tggagtatag gcttagagaa ctttctttct tgaataaagg aataaaaata
     5101 gtatttgaag ataaaagaga aggacaagaa aaaagaaaag aatttcacta tacaggtggt
     5161 ttggtagaat atattaagta tttaaataaa tctagaactg gtatacatga tgacatagtt
     5221 tatatagata aaaaggttga tgattgtttt gttgaactag ctatgcaata tacagatggt
     5281 tatacagaga acatatattc ttttgcaaac aatataaata cacatgaagg tggttctcat
     5341 ttaagtggat ttaaagcagc tcttactaaa actgtaaatg attatgctaa gagaaataaa
     5401 tttttaaagg aaaatgatgt taacctactt ggtgaagaca taagagaagg tcttacagca
     5461 gtagtatcag ttaaattacc agaacctcaa tttgaaggtc aaactaaaac aaagctaggt
     5521 aatagcttta tgagaggtat agttgatagt gtaacagttg atgaactggg gtctttccta
     5581 gaagaaaacc catcaacagc aagaatcata gttgataaag ctttaagagc acaaagagct
     5641 agagaagctg caaaaaaagc aagagaatta acaaggagaa aaagtgtatt agaaagtaca
     5701 tctttacctg gaaaacttgc agattgtgca gaaaaagatc catctaaaag tgaaatattc
     5761 ttagtcgaag gggattcagc gggaggttca gctaaacaag gtagagatag aaatagtcaa
     5821 gctatacttc cattaagagg gaaaatactt aatgttgaga aatcaagact agataggata
     5881 ctatcttcag atgaaataaa aaatatgata acagcttatg gttgtggtat tggagaagat
     5941 tttgatatag ataaggctag gtatcataaa attataatta tgaccgatgc tgatgtagac
     6001 ggagcccata taagaacttt attattaaca ttcttcttta gatatatgag acctcttata
     6061 gatgaggggt atgtttatgc agcacagcca cctttatata aagtgacaaa gcaaaagaag
     6121 gaacattatg tttactcaga taaagagtta aatatcttac tagatgaaat tggaagaaat
     6181 ggtgttgaac ttcaaaggta caaaggtctt ggagagatga atgcagagca attatgggaa
     6241 acaactatga atcctgagac aagaacttta ttacaagtta ctgtagaaga tgctgcaatt
     6301 gcagatgaag tattttcaat gcttatgggt gataaggttg ctccaagaaa agaatttata
     6361 gaagaaaatg caagatttgt tagaaactta gatatataga gagaggggat tagtatacat
     6421 ggaagaaaat aacaaaatac tccctattga aatagcggaa gaaatgaaaa aatcgtatat
     6481 tgattattca atgagtgtta tagctggacg tgctcttcct gatgttagag atggtttaaa
     6541 gccagttcat agaagaatat tatattcaat gagtgagtta aatttaactc cagataaacc
     6601 atacaggaag tcagctcgta ttgttgggga cgttttaggt aagtaccatc ctcatggaga
     6661 tactgctgtt tattatgcta tggtaagaat ggcacaagat ttttcaacta gagcactttt
     6721 agtagatggt catggtaact ttggttctgt tgatggggat tcaccagctg ctatgcgtta
     6781 tacagaagct aaaatgagta aattatcatt agaactacta agagatattg aaaaggaaac
     6841 tgtagacttt aaaccaaact ttgatgagtc gttaaaagag ccttcagtat tgccagctag
     6901 atatcctaat ttattagtaa atggctcaaa tggtatagct gttggtatgg caacttcaat
     6961 acctccacat aatttagcag aagtaattga tgcaactgta tatttgatag ataatccaga
     7021 gtgtagtgta gatgatttaa taaaatttgt tcaaggacca gatttcccta cagctgcaat
     7081 tataatggga aaagaaagta tagcagaagc atacagaact ggaagaggaa aagttaaagt
     7141 taggtctaga gcttttatag aagagctacc aaaaggaaaa cagcaaataa tagttacaga
     7201 aataccttat caagtaaata aggctaaact cgttgaaaga atagcagagt tagttaaaga
     7261 aaagagaata gaaggtatat cagacctaag agacgaaagt aatagaaatg gtatgagaat
     7321 tgttatagaa ttaaagaggg atgctaatgc taatatagta ttaaataatt tgtataaaca
     7381 ttctcaaatg gaagatactt ttagtataat aatgcttgca cttgtagatg gtcagccaag
     7441 agttttaaat cttaaacaaa tattatatca ttatattaaa catcaagaag atgttgttac
     7501 aagaagaact aaatttgaac taaataaagc tgaagcaaga gcacatattt tagaaggatt
     7561 aaagattgct ttagataata tagatgctgt tataagcttg ataagagctt caaagactgg
     7621 gcaagaagct aagctaggtt taatagaaaa attcaaatta actgaaatcc aagcacaagc
     7681 tatattagat atgagacttc aaagacttac aggtttagaa agagataaga tagaagctga
     7741 atatgaagat ttaatcaaga agataaatag attaaaagag attttagctg atgaaagatt
     7801 acttttaaat gtaataaagg atgaaattac aataataaaa gaaaattact ctgatgagag
     7861 aagaacagaa ataagacatg ctgaaggcga aatagatatg agagatctta taagtgatga
     7921 agaaatagca ataactctta ctcactttgg atatataaaa aggcttccat ctgatactta
     7981 taagagtcaa aaaaggggtg gaagaggtat ttcagcactt acaactagag aagaagattt
     8041 tgtaaggcac ttggtaacta caactactca tagtagacta ttattcttta caaataaagg
     8101 tagagtattt aaattaaatg catatgaaat accagaaggt aagagacagg ctaaaggtac
     8161 tgctatagtg aatttacttc aattgtcagc agatgagaaa attgctactc taatacctat
     8221 tgatggtaat gatgaaaatg aatatttatt acttgcaacg aaaaaaggta ttgttaaaaa
     8281 gactaagaga gaagagttca aaaatataaa taaatctggt cttattgcta taggtttaag
     8341 agatgatgat gagcttattg gagtagaact tacagatgga aaacaagaag tacttttagt
     8401 aactaaagaa ggtatgtcta taagatttga tgaaaatgat ataagatata tgggtagaac
     8461 agcaatgggt gtaaaaggta taactttaag taaagaagat tttgttgtgt ctatgaacct
     8521 ttgcagtaaa ggtacagatg tgttagttgt aagtaagaat ggttttggaa agagaacaaa
     8581 tatagaagag tatagaagtc aaataagagc tggtaaagga attaaaactt ataatatatc
     8641 tgaaaaaact ggtacaattg taggtgcaga tatggtcaac gaagatgatg agataatgat
     8701 tataaattct gatggagttc ttattagaat aagagtcaat gaaatatcac tgtttggaag
     8761 agttacaagt ggtgttaaat taatgaagac gaatgatgaa gtcaatgtag tttcgattgc
     8821 caaaataaat attgaagaag aataggataa atatcctatt ctttttgtat aatgtagtta
     8881 gataaaaatt taaaattgaa aggagtaatt gttatgtgca ataaaagaag ttgtaaaaaa
     8941 ggtggatttt tattattagg agctatttta gggtttatat ttggtatgtt ttttgcacct
     9001 aaaaaaggtt ctgaactaag aaaagaaaca aaagagaagt ttaatgatgt taaagaaaat
     9061 ccaaaagaag ttctacatga aacatttaat gatgttaaag agagaataat aaatttagtg
     9121 gatgatgata ataatgaaga agatataaag atttcagaag aagatatagt aataagtaaa
     9181 agctttgatg atgagggaga tgttaactaa tgaatgcatg ggggtggcaa attggagcag
     9241 tgttagtagg aagctctgct cttatagtag caatttattt agctaaaact ctaaacagca
     9301 taaataaggt tgttgaaaag gcatataaga ttgtagatta taatgaaaga catatacaag
     9361 atattgttga aaatgcatca tcaatatcaa agggaataga tgatatagtt tatgtaatta
     9421 ataaaatatt gagtatagga aatatattta aaattattaa aaggaaatag gaggttttta
     9481 catgtctatg aatattgatt caaatttaga ttcacaaaat aaattttgga atgtttgtct
     9541 tgatggagaa cttgatgttt ctacagcaga taaactaaag gaacatttac atgctttaat
     9601 agaaaaaaat atgttagatg taaagattaa tcttaaagat ttagattata tagactcaac
     9661 aggcttagga gctatgatag gtgtattaaa aaaattaaaa ataaatgaaa aagagatata
     9721 catagtaaat cctaagagta acgtaagaaa gatttttact ataacaggtc tagataaaat
     9781 atttaaagtg gagggataaa ctttggcttg tgagactata aagatggaaa ttactacaaa
     9841 tccagattat gttagtataa taagattaac agcatctgga attgccaata agatgggttt
     9901 tccaatagat gatatagaag atataaaggt agcagtatct gaagcatgta caaatgcaat
     9961 aaaacacagt aaagatgatg tgttttatat agtatttaat attttagata atgcaattaa
    10021 tatagaaatt caagataatg gaaaaggata tgatgttagc tcaataagta caccagattt
    10081 agagaatcca aaagagagtg gtcttggatt gtttataatt aaaactttaa tggatgatgt
    10141 agatattgaa tcagaacata atcaagggac aaaaataaaa atgactaaat atttaggagt
    10201 tgatatttaa tgaaaaatgt agctaatgct acacattacc taaatatgga tacaaaagag
    10261 ctatttaatt tatataataa aaataaaaat gtagacatta gaaatattct tattgaaaga
    10321 catctgtatt tagcaagatt gttagctaag aagtatataa acaaaggtgt tgattttgaa
    10381 gatatatatc aagttgcttc attggcatta atttatgcta ttgatagata tgatgttgag
    10441 aaaggatttg agttctccag ctttgcaaca ccaactatag taggagaaat aaaaaagtat
    10501 tttagagata aagtatggac acttagagtc ccaagaagaa tacaggaatt aagcaaaaag
    10561 ataagtgatg cgaagattaa attagaacaa gaaaataaga agcatccaaa ggtaaaagat
    10621 atagctgatt atataggcgt tagtgaagaa gatgttttag aggctatgga ggcttcttat
    10681 ggatatcaac caatgtcttt ggattcatca agtaatgatg attcagagga taaagatata
    10741 actcttattg ataagattgg aaaagaagaa ggtaactttg gaagtataga gtatgaagat
    10801 tttattaata agtttattga aactttaaat gaattagaag ttaaaatttt taaagataga
    10861 tttttctttg ataaaacaca atctagtata gctaaagaac tagggatatc acagatgact
    10921 gtttctagac tagagaaaaa ggtagtagag aagctgaaaa aagaatatga aaaaaattta
    10981 taaaaaaata taaaaaaagt attgacctac tgttttaaag atggtatagt attacttgtc
    11041 cttgaaaaaa ggaacaagaa ataaacacga acattgaaaa ttaaacagta ggttaattta
    11101 tat
//
LOCUS       DAKBNN010000131        11087 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000131 DAKBNN010000000
VERSION     DAKBNN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.132"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(48..668)
                     /locus_tag="OUB02_002718"
     CDS             complement(48..668)
                     /locus_tag="OUB02_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCT8886462.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(982..3120)
                     /gene="vanT"
                     /locus_tag="OUB02_002719"
     CDS             complement(982..3120)
                     /gene="vanT"
                     /locus_tag="OUB02_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HCT8886463.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGVKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3138..3944)
                     /locus_tag="OUB02_002720"
     CDS             complement(3138..3944)
                     /locus_tag="OUB02_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCT8886464.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3941..5041)
                     /gene="vanG"
                     /locus_tag="OUB02_002721"
     CDS             complement(3941..5041)
                     /gene="vanG"
                     /locus_tag="OUB02_002721"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HCT8886465.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5302..6384)
                     /locus_tag="OUB02_002722"
     CDS             complement(5302..6384)
                     /locus_tag="OUB02_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8886466.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(6434..7135)
                     /locus_tag="OUB02_002723"
     CDS             complement(6434..7135)
                     /locus_tag="OUB02_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCT8886467.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7569..10100)
                     /locus_tag="OUB02_002724"
     CDS             complement(7569..10100)
                     /locus_tag="OUB02_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HCT8886468.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10208..10825)
                     /locus_tag="OUB02_002725"
     CDS             complement(10208..10825)
                     /locus_tag="OUB02_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCT8886469.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
BASE COUNT         3736 a         1990 c         1158 g         4203 t
ORIGIN      
        1 ataatttagt actaaatcat tttaagatac gttttatttc gtacaagcta aatcatttta
       61 tcatctttat cattgtgatg ttttttatga tggaaatcat aattatgatt ttcaagattt
      121 ttatttactc tatttccaat cttccatgac aaaaacacca ataatataaa tgatgctatc
      181 tttaaaggac ttgttataaa accttctata tcacttgcca cgtaacttat aactataaac
      241 attacaaatt ttcctgccaa cattgctggc acaaaatctt ttaaatcttt tttaccaaaa
      301 gctgatgcga tagtaactaa acaaccgggc ataaatggac aggcatacgc tataaataat
      361 aacttaaatc cctgtttatc catccaactt attgcttttt cagttttact atttctcaac
      421 ttattaaaaa gcttactatc attaaatctg cttattaata agaataatga agttgtccca
      481 agtcctgagc caatccatga taacaaacat ccaataaata atccttgtat tgcagcattt
      541 gctgtaacta tagcaacaag tggtaaggct ggtataaagc tttctataaa tgcacttaat
      601 aagccgatta ttgtggctaa cacccaataa ttttgagtca aatcaagtaa ttcttgtata
      661 tactgcatat tgcttttccc cttttttaac tttaactatt attatatact ctatatgttt
      721 attatatacc aatctcaaaa aatgaatcta atattaaaaa gtaaataatc tatataagtt
      781 tacagttaag gtataattat tttattatat attataacaa aaaaggtgat gtttcaaaat
      841 aaaaatctga aacatcacct ttttatagct taaattgtca tgtaagttat aaaaaatata
      901 tattagacat aatattacta aaaatgtcta gtcagattaa aataaattac acagtacaac
      961 aaatattact atttaattga gttacttctt tataacattt aacctccttc ctactctgct
     1021 caataattca tttgatatac tacacgagtt atttgcaact tcagaagcag ataattcaga
     1081 taaattatct cttcctatta ttattgccgt attaccaact tctacattag gtatatctgt
     1141 aatatcaaca gcaagttggt ccatgcaaat tcgacccact attgctgctc tacaaccatt
     1201 tattattacg taactttttc cacaggataa atttctaggt ataccatcgg catagccaat
     1261 agataatata gcaattacgc tatctctgct tgcaataaag actcttccat atccaaaact
     1321 atcatctttt tgtatttttc taataagaat tacttgtgat tttaaggata aaacaggtcg
     1381 taaatcaagt tgcaatttag tcgtatcatt tggagaactt aatatcccgt ataatgcaat
     1441 tccaattctt acataattac acttaagatt gggataatta aaaaaaccat aactactttg
     1501 aatatgtact ttaggaattt ttacaccttt ttttaacaga aaatcaatta acttataaaa
     1561 acattcaatt tgtttatttg taaagtctac atcttcatct ttcagactat ctgaaacaca
     1621 caagtgtgta aatatgccac atatatctaa gtattttaat ttaaatgcct ttaaaattga
     1681 cctcacatca ttaacatcaa aacccagtct gtgcatacct gtatcaattt taatgtgtga
     1741 ttttatgttg tatttctgtt tgtttaaaga gatagcatga ttaaggtcaa taacactctg
     1801 tgtaagattg tatttatgaa gttcctttgc ccttattggg ttagtatatc caagaatcaa
     1861 tatttctcct agtattccat atcgacgtaa atcaatcccc tcatctattg ttgctactgc
     1921 aaatgctttt acacctattt ggtttgcaca agtcgctact tcaaaagcac tatgaccata
     1981 ggcattagct tttacaactg ccatcaattc acaatcttct ggcatagctt tttgtatcat
     2041 ctttacatta tgttccaaat tatttaaatc aatttcaatc caagctctat tggtatttac
     2101 cttatgttct cctttcaagt ctttttttag aggtttaaga atcaatataa taattataga
     2161 agctataaaa gatgctatac ttaccactat ataatgaata aggctattgt caaccaagac
     2221 agtttgtatt ccgaatattt tagaaaacat acgaataaca acaatcatca taggatgtat
     2281 aatataaata attaatgatg atgttcgcaa taacctagct cgagaacctt tcaaaaatgt
     2341 taaggctgta aataagaaat acatacatgg caataacata atatacatgc tatcatgacg
     2401 ctgtacccct agtttatgta aaaacattcc ctcacataac ataagtaaga atgatataag
     2461 aaatccaatc aagctatttt tagttgacat ataattagat ttattagata ttattccacc
     2521 aagtatgaaa aatacaggtg caaaaaatat gccatttcgt gtataatctg atacttcaaa
     2581 aatataccca tataaattct ttaatagtgg tattttttct aaaaaaccat aataactatc
     2641 accaaacata ccaattacat aaataacaat agtgattact agtgagtact ttaaacctag
     2701 tttttttaca agaaagtatg caatacttgc accaataatt gatgcaggaa gataccataa
     2761 atgatataaa gtcccatcaa atacaatgtc cttaataata ttgggcacta aattattcat
     2821 ggaaaaatat ccagtgtaaa tatttaaggg aatataaatt acaatagcta tcccgtaaat
     2881 aaataataca ttttttagaa aagatttcag tttattacaa ttataattat atgttgaaat
     2941 tagaaaaaag cctgatagca taaaaaagaa tggcacagca atacgagcaa ttatactagt
     3001 taacacaaag tcagccattt tactatatga taataatgga gatgtatgaa ttgcaattat
     3061 aagtaatgca gctataatcc taaaataatc aagccctgca taagatttat tcctaatcat
     3121 tcttgttcct ccatttctta gtcctttcta tttctccaaa atgtcacaat aaaaccatcc
     3181 ttattgttac ccgatatttg atatacacat tgttcaggta tagagattct cacttcttct
     3241 ttagtatgaa taggcacata aaagatttct ataatttttt ctttatcttc tatttttaaa
     3301 caatttgtat acgaaaatct cttaatatat tctatatatt cttcaagagc gagattcatt
     3361 ttattgataa tctcagaatg cggatatccc acataacgaa aatgccatgg ttcttgagat
     3421 attcctgtta tttcttcttt tttacactga taacgctcta taaaaccata atgtagtgct
     3481 gccttacgaa actcattaca aataccatta taaggaaatt cagggcagat aaaatctata
     3541 acttctttct ttaaccctaa atcaatagca agccctgtct gatgttcact gtgatttgga
     3601 agtgctacaa actttttagt aaagtctttt ccattgtcac gcagagaact tgaatatata
     3661 tcttgttgtt cctctgcagt acgatatcca cttacaggta ctatttcatt tattccacat
     3721 atacttttta aaatattttg taaaatgttt gtagcttcac gtttcataag aatacttgga
     3781 aaccttgtat ctacaggaat taaatctata tcttcagatg ttttaactgg taaaaaagca
     3841 tttactagaa ttagattccc atcataaact ttatctttag tcaataagat agtatttgtc
     3901 attttatctt ctttattttt atctaataaa atattattca tcattttatg tacacctcaa
     3961 ttatcttgtc caatatttct tcaaaagaca gtccaatacc tttcatcata tttggataac
     4021 gactatgaga agtaaagcca ggaattgtat tgacttcatt aaaaataatt tcaccagatg
     4081 gagttaaaaa catatctact ctagcaaatc cagaacatcc aagagttcta taaataattt
     4141 ttgctgtctc ttgtattctt ttctcacaat ccaaatcaat tctagctggc atatgaattt
     4201 ttgaactttt caatgtatat ttttctgtat aatcaaaaaa tccttttgac aattctattt
     4261 catctactct tccaacaagt aagtcattgt ttcctaaaac tgcacaccct acctcaaaac
     4321 catcaatatt ttcctcaatt attacttcat tatcatgttc aaaagccaag tctactgcta
     4381 cttgtaactc ttctttttta taaattttac taataccaaa tgatgaacct gcacgcacag
     4441 gttttataaa taatggataa gttaagtctt ttgacagttc agccacatta aatttatctg
     4501 agtattttag taatattgat tttggtactt taataccttc tgaatgaaca agtttatgtg
     4561 atttgtcttt gtccatacat aatgctgaag aaagagtacc acatccaata acctgtatac
     4621 ctgccaattc aaataacccc tgcactgttc catcttctcc attttttcca tgcaatacag
     4681 gaaacacaaa atcaagttta gtgtatttaa tatcattttc acatgattca atagcactat
     4741 ttggaatatg attctgagaa acagcatcag gaattaaaaa ttcaataata ccatttttga
     4801 cacggttctg cgaaacaata actggaatta tatcagtatc cctttcaaac caagtgttat
     4861 tctggatatt attataatct ccaatataat gataccaatt accctccctt gtaattccaa
     4921 ttggtataat atcatatttt tccgtattca aattctcaag tactgcaaaa gctgattgta
     4981 atgatacatc atattctgtt gagtttccac caaatataac tgcaattttt ttattattca
     5041 tctttgattc tcctttgttg atttatttat ttttatacaa tctacagaaa catgttaaaa
     5101 tactttttct atttcaaatc caatcaacct tcacttacaa ttgcatctgt tccacataat
     5161 atattttcac atttttctat tgatataatt actgtatcaa aataaaagca ctcttgtttt
     5221 gatacagtaa ttatatcaat aacaagagtg ctctttattt atttatccta aagaatttcc
     5281 tattttttta tacttaaatt cttacaaatt tcctacaatc ggaataataa ccttaaaggt
     5341 aattatattg ttctcgctat gagctgttat tgtcccgtta tgaagctcca ctatttcttt
     5401 agctatagca agtcccaacc cagctctacc actgtttgaa ttacgagatg tatccaacct
     5461 gtaaaattgt tcaaaaatac gctcaagttt ttcctctgga atcgtattgc tatgattcat
     5521 aaatttaagc tttatataat catcctcctg tacagctgtt atttcaatag tagtatcttc
     5581 aaaactataa taaacagcat ttcgtaataa gttatcaaat acacgttgca ttttattaac
     5641 atcacacctt atcatcatat ttggtgtaac attcagtgta tattttaaat tcttactcat
     5701 caaagtaggt ttaaactcat atgttagttg ttcaagcata cgagttaagc ttactgtact
     5761 gtattcaagt gatatatttg ataaattaaa ccttgtaatt tcaaagaatt cattaattaa
     5821 atcttcaaga cgctctgcct tatccaacga aatagataaa tatttttttc gcagttcttc
     5881 agaaatctga ctttcatctc gaagtagtgt cagataccct ataactgatg taagtggtgt
     5941 ttttaaatca tgtgcaagat aaacaataag ttcattctta cgttgttctg ccatttgtgt
     6001 ctcaaattta cgttgttcaa gtatatgttt aattgagttt attttctttt ctattgccaa
     6061 taactctgga gataaagcca cttctccaac atcttctttg ataagagagt caataccttg
     6121 atttatttca ctaaaatatt ttgtaaacca atttaaataa acacggaata ttataaaaaa
     6181 cacaatcacc atagctagta tcatgaagag ctccatgcta ttacggaaaa tctgctgata
     6241 taaattcaat gcagctttat attccatacg aaaagcattt tcaaaaaatg atactatcca
     6301 gatagaaaac ttaccataca atataaaaga atataaaatc catatgaaaa taatggctat
     6361 aaatgccatt aaagctgttc tcataaaaat tttatttctc acttgagtaa agtcaggatt
     6421 gactttaata tttttacttt tcaattgtat aacctactcc ccatacagtt tttataaatt
     6481 tcgggttttt agaaggttca tgaatttttt cacgtaatcg ccctatatga gccataactg
     6541 tgttattatt atcaagatat ttttctcccc atacagcttc aaacaattct tcagaaggaa
     6601 ccacttttcc ttgatgttca cataaatacc aaagaataga aaattcaatc ggtgttaatg
     6661 ctacttcttt cccaaataaa ctacatttat gtgtttcttt attgataatc aatcctttaa
     6721 tatcatactc attaacaatt atcgactgtt gttcataaga attattatat cgcatatatc
     6781 ttcttaattg tgtctttact cttgcaacaa cttccaaagg gttaaatggc tttgttatat
     6841 aatcatcagc acctatggtt aaacccatta tcttgtctat atcttcaatt ttagctgtta
     6901 acataatgat aggataatag tatttttctc gaattttctg gcaaatatga aatccatcaa
     6961 tatcaggaag cataatatct aaaatagcca tatccaaatt tttagattca atacattcta
     7021 gagcttcctt acctgtataa aacttgtata ctgtaaaacc ttcatttttt aaatatactt
     7081 caattaaatc tgctatttct ttttcatcat ccacaatcaa aatattttcg ttcatagatt
     7141 atttttcact ctccttatta tcgaggtcat catttataat attggtaaat ttacatatca
     7201 aataattaca agcatatttg taactaattt ttataagaaa agaactacta catcagaatg
     7261 tgttgatgct accattggaa cacaaaaact gagattaatt gttattataa aaactcccct
     7321 aacacgtcct tattatcgca taacaggtat taactttcaa taaaaatatc tatcagtcaa
     7381 acacaattca atttattata ccagagacaa tccatataaa aaataattaa acctatgtat
     7441 gtaaccacag acattactaa aatttccatg ttattttata taaaaattca aaaaaggagt
     7501 tgaatttaaa atttgattag aaaaaaatca attttgagac aactcctttt tattttaatg
     7561 tatttaaatt atagtatatc agccttcata atatcctcaa aatcaccttt ttcagatatt
     7621 aatcttatca atttaacagt acagcttaaa tcatttataa gtatagctcc aactaatgaa
     7681 ccatctttca taaataattt tttatatatt ctttgacctt catcttttga tgaaatttct
     7741 ttatcaaaat tttgtatatt accaatacta aatactttta cattcattgc atctaatgaa
     7801 actggatata cttcactttc aaatgtcaaa ttatctccac ttgcatttgc ccctgctatt
     7861 tttcccattt ctactgaaga tggccatatt gctaaactaa tatttcccac ttgtgcaaca
     7921 tctccacaag catatatatc tttaatattt gtctgtaatg tcttatctac aacaatacct
     7981 ctatcaattt ttacagatgt atcctgaacc atttgagtgt ttgctcttac accaattgaa
     8041 aatactacca tatcacagtc aacactatct ccatctttaa atacaacttt ttgagctttt
     8101 ccatcacctt caataccatc aactgctaca cccaatctta catctattcc tgtatctctt
     8161 acacattgtt ctaatatttt actaccttct ttatcaagtt gttttgatag tatgctatcc
     8221 atggcttcaa ctactactac ttctaatcct tttagtctaa attcccatgc agcttcaagt
     8281 cccaataaac cgccaccaat cactacaact ttcttagatt tttctagtgc ttcttttaca
     8341 ctatataaat cttttatatt tctaagagta aacacatttt ctaaatcatg acccttaatt
     8401 ggtggtataa agtttctact tcctgtagct attacaagct tatcaaattt aatagctcca
     8461 tcattcacta taatagtttt attcacttca tctaatttat ctacattcgt tcctaatatt
     8521 actactatat tatttttatc ataccaatct ttatcttcca tgtagaaatc tgaaccaagt
     8581 tcttcattta taccatcaga aagtgcaggt ctatagtaag tcaactcatc ttcattagaa
     8641 atcatagtta tcttacaagt tttatttctt tttcttatag catctgctgc ataaaaacca
     8701 gctgctccat ttcctactat tacgaaatat tcatcagtat cttttctaaa agtaacttca
     8761 tcttcttcaa cttctataaa ttggtcttcc ttagctccac atacagggca tacactagga
     8821 gcactatcac cttcaaaaac ttcattacaa attacacatt taaatttctt agttttcttt
     8881 ttacttccaa aagtagaatt taacttatct aggaatttac ctgtataaga atgtaattta
     8941 tcaatctctt catttgatgg tttaaagtta acagcaaaag gttttactgc tgtcattcta
     9001 agctgactta ttctctccat agcattttct ataccttctc cactccatcc atatgaacca
     9061 aatactgaag caacttttcc accatgaaga actgggttta acgatattaa tatatcccat
     9121 ataggcttta acgcatctcc taatattgtt ggtgttccaa ataatacacc ttgtgatact
     9181 gctatttttg ccaaaatatc ttcttgctta tgctctatta catcaaataa atttacttta
     9241 tagttgctat ttttttcaat atatgctttt atagcttctg ccataatctt tgtatatcca
     9301 tgtgcagaaa cataacaaat tgtaacttct ttttctaact ttatttgttc atttacactc
     9361 cagttgtaat aaaggtctat aatttttcta ggattttcag ttaatactgg accatgacct
     9421 ggacatacag tatctatatc taaatctttt atcttttcta tagcagttac catagatggt
     9481 ttaaatggac ccattataca atcataatag tatcttaaag catccaagta atccttttct
     9541 tcattttcat caagtgtatt tactatctta tcattactat agtgactacc aaatgaatca
     9601 catgttacaa gagttttatc ctcttttata tatgtgtata tggtatctgg ccaatgaagc
     9661 ataggtacag agaaaaattc taaagttttg tccccaattg ataatgtatc tccatctgta
     9721 actgcaacat attcaaagtc tttgtttacg atttccttaa gatatttaat tgctgtttct
     9781 gaagctacta cttttgcatt tttagctaaa tccaaaagct tttcaacgct tcctgcatgg
     9841 tctggttcag tatgacttac aacaatatag tctatttttt caaaatctat atttaaatca
     9901 tttaatcttt caatataatt gtcaaaatgc ttatctttaa ctgtctcaaa taaaactgtt
     9961 ttttctgttc cttttaatac atatgaatta taagtagttc cataaggtgt atacataatt
    10021 atgtcaaata ctcttaaatc agggtcaagc gaacctaccc aatataaatt ttcttttatt
    10081 tttagtgatt tcaatgacat accgtactcc tcctaaaata gttatttagt ctctttatac
    10141 cctgcttgtt atattttaaa caaaatttat tcaaaaaaga aattatttta aagatagttt
    10201 aaatcttcta atcttctttt tctaccttca atacagttcc ctttttagaa tctactttta
    10261 tgtcatattc atttttatct ttatctattc ttattttata aaaagtaaca tctaaatctt
    10321 tgtctaaact ccattcttta actattccat tttgttcttt taatgcaatt tccatagctt
    10381 gttgaggtgt cattatgtca tttaaatcta atttttcttt tctagcaact ccatttaaat
    10441 cttctgaatc caacttttct gttttatctt ctaatacatc ttttgtatta gcatcaattt
    10501 tcatcttata ttcattatta tcatctacac cttccacagt ataaacaaat ttatttaaat
    10561 ctctctcaag acttaaatct actatatcag catccttata tttatcttta aatatgttta
    10621 ttgcatctgt gtatggcact ttaaaatctt gttccattaa attttcatta tttttttcat
    10681 cttttaaagc gttatttgat gcatttgcct ccttttttgt attatcttgt gcttgtcctg
    10741 aatccttagt tggactccca ctacatgcag ttaataaacc cgctataagc atacttacca
    10801 ctaataaata cgttttttta cacataaaaa ccctccttaa atatattaat ttattctata
    10861 ttatgccctc taaacccttt atttaccctt ttatattaaa aattcctcct attttatcaa
    10921 ttttaaaact ttatatttac taaaattaaa gttacataaa aattatactg tctcaaaatg
    10981 gaaacaagcc tctttttcat tgttgaaata ataaaaatat attttatttc gtacaaaaag
    11041 agtgcctcat gaactaaaaa gttcattttg agacactccc atttttt
//
LOCUS       DAKBNN010000132        11057 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000132 DAKBNN010000000
VERSION     DAKBNN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.133"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            513..1373
                     /locus_tag="OUB02_002726"
     CDS             513..1373
                     /locus_tag="OUB02_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8886470.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1363..3234
                     /locus_tag="OUB02_002727"
     CDS             1363..3234
                     /locus_tag="OUB02_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8886471.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3254..4708
                     /locus_tag="OUB02_002728"
     CDS             3254..4708
                     /locus_tag="OUB02_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCT8886472.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5733..6941
                     /locus_tag="OUB02_002729"
     CDS             5733..6941
                     /locus_tag="OUB02_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886473.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6972..8351
                     /locus_tag="OUB02_002730"
     CDS             6972..8351
                     /locus_tag="OUB02_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886474.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8457..9647
                     /locus_tag="OUB02_002731"
     CDS             8457..9647
                     /locus_tag="OUB02_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886475.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9775..10839)
                     /locus_tag="OUB02_002732"
     CDS             complement(9775..10839)
                     /locus_tag="OUB02_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886476.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
BASE COUNT         4123 a         1237 c         1982 g         3715 t
ORIGIN      
        1 ttatatatta gtatatctta ctagcattta aatgaaacta aatttgaagc tttacctatt
       61 aatatatctt aatagtgatt aaataaaact aaacttaaga ctttatatgt taatatatct
      121 taagaattta tttgttaaca tacagttcat tatatttaaa agcattacta tgataattca
      181 taatagtaaa atagataatg gttcagacaa tattataaat attagtgacg gtaaaatagt
      241 atcaatttag tatataaacc ttgtcagata atcgcataac agagcttgta gttatagaaa
      301 aaaaaaatat ctaggaaacc gttgacagat ttatatggat agtataacat atagaatatg
      361 aaattaaaca aaaaagaatt ttaattatgg cgacgtagtc ataattttga gtatgttact
      421 gattagcagg cagaactcaa gtatatatgt gaaaaataga gggatactct atacatatat
      481 gcttgagttc tttttattga agggaggaat atatgaaaat aattcagatt ttaaataata
      541 atgtagcctt agtaaaaaga ggtaaaaatg aaattattgt atttgttaaa ggaattggct
      601 ttaaaaaaag agtgggacaa gtaataaatg aaaatgaaat agagagaaca tatatactag
      661 attcatacga tatgctagaa cactttagtt atctactaca aaactcagac tccaataata
      721 tagttcttat taatgaaata attgaatatg gtgagaaagc tttaaatata aagtcaagtg
      781 attatctaag cttaacctta ctagaccatt tagagttttt acttaaaaga tgtgaaaaga
      841 atcaatttat aaaaagtcct ttggtatgga atgttaaaag attttatcca aagcattttg
      901 aaattggaat gcatgcacta aaattaattg gtgatagtaa gggaattaat cttcctgatg
      961 atgaggcagt atcaatagca cttcattttg taaatatgga ggtaaataaa gagtctcatg
     1021 atagtacaat tgtagagttg agaactttag cagatatagt ttctataata aaatatcatt
     1081 ttaatgtaga gctagatgaa acttcaacaa attatatgag atttactact catctacagt
     1141 actttatcca aagaattatg tcggatgaag cttcaataga tgaagataat tctatagatt
     1201 tatataaaca agtaagtcaa ctttatccaa agtcatttgg aagtgttcaa aagataagaa
     1261 aatatataaa atcacaattt aatgtcgata tatctataag tgaagaaact tatttaatgt
     1321 tgcatataaa tagggtgact caaagactgg aggcaaaatc agatgaaata taaagattta
     1381 aatgaaaata taataaaact tataggggga aaagaaaata taaagtcggt agcgcattgt
     1441 gttactcgtt taagatttac tttaaatgat aggagtatcg ctcaaacaga agaaattaaa
     1501 aaattaaaag gagtaattga tgtagtttct aatgatgttg cttatcaaat tataataggt
     1561 actcatgttg tagatgtata taacgagttt atgtctatga taggtttaag ttcaagtgaa
     1621 tcaagtataa aaagtgaaag aaaaaaggga attagaggta tcaaaggtat atttaattcc
     1681 atctttgtgg tagtctcaga gactatgact tcagtaattg aagtactact tgcagctggt
     1741 atacttgcaa gtatattagc acttttaaat atgtctggca tacttcagag tgacagtcct
     1801 acatatcaaa tattagatac attaagaagt gctacatttc acttcttacc agtattaatt
     1861 gcaatagcat cagcaaaaag acttaatgta aatccctata tagcagtagt gttagcagtt
     1921 acgttattat cttcatcaat agatggtgta gaaggattaa gtttatttgg tataaattta
     1981 caggctacaa cttatgcaaa cagttttatt ccaatacttt taggagtatg ggcaatggga
     2041 attataattg aaggtttgaa aaaaatatta cctaaagcac tacactattt tttagttcca
     2101 gtattatctt taataataac tctatcagtt acattaactg tatttggacc tattggtatg
     2161 tggattggtg atggattaaa ctatgcatgt atgtttttaa ttgatacatt gggtaattgg
     2221 agtgttgttg cactgtatgc agctttacaa ccactcttaa tagtttttgg tgcagcaaac
     2281 tttactatgc ctatatcttt aaattctgtg acaacattgg ggtatgaccc aatttttatg
     2341 ggcgctgcaa caatatctga tattgcagtt tgtggtgcaa tgtttggata tttcttaaaa
     2401 actaggaata aagaacaaaa acaactattt ggtacagtaa gttttagtgc acttatggga
     2461 gttacagaac cttcagtgtt tggtgtgttt atgaaatata gaagaccatt tgtagcagta
     2521 atgataggtg gaggtatagg tggtttaata gcaggtttag ctcaagttaa aacatatgga
     2581 atggtttggg gattagctgc attaccaaca tatcttgttg gaggagaagt atctaacttt
     2641 acatttatga taataagtgt agtagtatca tttgtagcag caacagcagc ttcctattta
     2701 ctaggaatac cttcagatga aaaagaagaa gttaagggtg ataatgaaaa agaattagaa
     2761 ctaataatca acgagaacaa ttcaagaact attagtattg gaaatgttgc aaaaggtcag
     2821 gtaattgcac ttggggaagt taatgacaaa gcattttcat ctggagcatt aggtaaaggg
     2881 gttggagtta tacctagtga atcaaaatca actgttatat ctcctgtaga tggaactata
     2941 actatagtat ttccaactaa acatgcatat ggtatagtga ctgatgaagg attagaaata
     3001 ttaattcata ttgggataga tactgtaaac ttagatggaa aattctttga aagtcttgta
     3061 acacaaaatc aaagggttaa aaaaggagat gctttagcag tatttgaatc tgataaaatt
     3121 atagaggaag gctttgattc tactataata actgtagtta caaatactag cgattattta
     3181 gatgtaatct catcaaatga tgaatcagaa gaattattaa ctgtaatagt ataaatttta
     3241 ggagggaaaa ttcatgaata ttaaagatac atttttctgg ggaggaagta ttgcagccca
     3301 tcaatgtgaa ggttcatggg atagtgataa caaaggacct gcaattatgg attttgtaac
     3361 taagggctct tatgaaacgc caagagtaat tactgataaa atagaagaaa aattggatta
     3421 tccatctcat aatggaatag atttttataa tagatataaa gaagatatag ctttatttaa
     3481 agaaatggga tttagtgcat taagaatatc tatagattgg tcaagaattt ttccaaatgg
     3541 agatgatgaa aatcctaatg aattagggat taaatactat gaaggtctga tagatacatt
     3601 aatagaaaat aatatagaac ctattgtaac actatatcat tttgaattac ctatgaattt
     3661 agtacataaa tatggttcat ggaataacag aaaacttata gatttgtatt taaagtacag
     3721 tgagactgta ataagaagat ttgatgataa ggtaaagtac tgggttacat ttaatgagat
     3781 gaaccatatt gaccctcaaa cagagcactc ggatattttt acttatatga tagcaggtat
     3841 taagtatagt gaaatagaaa ataaggttca aacattggca aatgtagggt ataatatgac
     3901 tttggcgggt gtaaaggttg caaagttggc tagagaaatc aatcccaaca ataaagttgg
     3961 atgtgtattt ggactaaatc caatttatcc atacaattgt aatcctagta atgtgttaaa
     4021 tgcattttta gataatgacc gtgattatta tcaaattgat gcaatgtgca atgggaagtt
     4081 tccacaatat aaattaaaag aatatgaaaa aaataatata actattgaag tgactgatga
     4141 agacaaaaaa gcatttaaag aaggtaagtt agattttata ggacttaatt attatttttc
     4201 aagtgtatct actccaaaag aaaatttatc aggtgataaa tctttatttg gtgggattca
     4261 aaatccatat ttggaacaaa gtaagtgggg atgggcaata gaccctatag gtttaagatt
     4321 taccttgaac tatatttaca gaaagtatca attacctatc ttaattacag aaaatggttt
     4381 aggggcatat gataaaatcg aagaagatgg aactataaat gatgattata gaattgaata
     4441 tttaagtaag catctatctc aaatgaagtc tgcaattgaa gaggataatg ttgaatgttt
     4501 tggatatttg atgtggggac caattgattt agtcagtgca actacaggtc agatgagtaa
     4561 gagatatgga tttatatatg ttgacttaga tgatgaaggc aaaggaagtt ttaagagata
     4621 taagaaaaaa tcatttgatt ggtacaaaga agtaataaaa tctaatggag aagttttaga
     4681 tttttctacg gatatagtag tagggtagga ataagataaa tagaagttag gaactaatgt
     4741 taattagtgt ctagtaaagc atttaaaata gagtataggg tctttaaagt aaagtgtgta
     4801 ttcttcttaa aagtaatggc aactaaaatt actataaaaa ggagaataca cattttttat
     4861 gtttatactt aagtaaatat ttagacatat tcatagtata atttactcat tatataaaaa
     4921 tagtatgatt tactcactat ataactttat aaattttctg gaaaaatgtc agaatatatt
     4981 gacaaagaaa agatttaaaa ttataatcta tattaagaat agtttgacaa tataaaaaca
     5041 agtattttta taaatagtga aattattttt attgtaaatt taagaataat ttaggactac
     5101 tataaagata aaggtataga tgtcattata tgataaatca tacttgtata gaaagaattt
     5161 taaagtatgg cttatcattg atttaatata ataaataatt tttgtttcta gtaatagttc
     5221 ctaggaataa gatatctaaa ttcgtttaga aatagtctaa tttacaaagg aggggatgca
     5281 ctaatagata aaatttctat ataaagaaga cttaaagtga agtatattct gataatattg
     5341 gtttaatggg tatattatta tttcgtttta gatattttaa gaataattta agacgtttat
     5401 aaaaatgtat tctatatcta atatttaaat aaatataagt cttccaacat gtatactaat
     5461 atagagagta gttgaagtac aattctaact cgtaaaatta tgtagaaagt aaaaatcaaa
     5521 aagaaatcta gtaagttgat aactatagtt aatatttaga gagttaactc tagttatatt
     5581 tatttagata ttttaggaat aatttaggac gtatataata tcatcaaaaa atatcaagct
     5641 ataccatatg tagttaagaa taactgcata atatacattt tataattatt gttttattga
     5701 taaaaaatta ttatatttag gaggaaacaa caatggaaaa tttttggaat ccacttactt
     5761 gctttactgt atgtgtaatg atttatgctt ttagtgagtt tttatctaag aaaacaaaag
     5821 gtgcaatttc aacattgtta tttgtatgta ttatattttt actaggattt tggacaaata
     5881 ttttaccaaa agacattaca ggagcatcag ggatattagc tgcaatgagc aactttggta
     5941 tagctttatt aataactaac ttaggaacac ttataaatct tgaagactta tgtcatgaat
     6001 ggaaaacggt tgtaattgca ttagtaggta ttgtaggaat aggggttggc tgtttaacag
     6061 tgggtagtat gttatttggt agagagtatg ctttaatagc agcacctcca atagcgggtt
     6121 ctactgttgc tggtattatc gtaacaactg ttgcagaagc tgcaaataga cctgaacttg
     6181 ctgcatttgt aattctagta ttatcatttc aaaagttctt tggcattccg ataactacat
     6241 attgtataaa taaagaatta aaatttaaaa gagataacgg ggattttttg aaagatgagt
     6301 caaaaagtaa ttttaaactt ccaagtatga gaatttttaa agaagattta ggtaaaaaat
     6361 catctacttt agtatatctt gcaaagcttt catttgttgc tatgattggt aattttgtag
     6421 gattggctac tttaatacca ggatttaccc ctgcaaacta ttatttaaat cccaacatag
     6481 catgtttgtt agtagggttg atttttacac gaataggttt tttagaaaaa ggaattttag
     6541 ataaatctca atctaatgga attataatgt ttggttgtat gttgatgtta cctggaggac
     6601 ttgctaaggt aactccaaca gcattgtttg ggatgattat tccaactata ggaatacttg
     6661 ttgtaggctc tgtatttata atactattta ctgcaattgt aggaaaacta ttgggatatt
     6721 ctccaagaat gtcagctgca attggtgtaa cttgtatgct agcttatcct gcaacacaga
     6781 taatttccaa tgaaggtatt gatgctatgg aaggaactga agaagaaaaa caaagggcaa
     6841 cagattattt attaccaaag atgattatag gaggatttgt tactgtaacc attgcatcag
     6901 tggcatttgc tagtataata ggtccaatta tatttggttg atagtaaata gatttatagg
     6961 aggaatagaa aatgatagga agtatatcta taaatgagat agataaaaaa agattcaaac
     7021 ttgatgattt aagtaagaaa atatgggaaa atccagaaaa agcatttaaa gaatttaaag
     7081 catgtgaaaa tacagcaaac tttctaagaa gtgaaggatt tgatgtggaa gtaggtgtag
     7141 gaggtttagc cacggctatt agagctagtt ttggttcagg taaaccagtt ataggtttta
     7201 tgggagaatt tgatgcatta cctggtttaa atcaaaaagt ttcaacaaaa caagaagcat
     7261 ttgaattggg tgcatatggt catggatgtg ggcataattt attatgtaca gcacatgtag
     7321 gggcagtagt agggcttaaa agagagatga tagaaaataa cttaagtggg actatagtat
     7381 tttatgcatg tccaggagaa gaacaattaa caggtaaagg atttatggct agaggtggag
     7441 cgtttgaagg gttagactta gctataaatt ttcatccaaa taaaattagt gaagctacaa
     7501 ttggtacttc aacagctgtt aactctgtaa agtttcactt taagggaaag actgcacatg
     7561 caggctcaga ccctcaaaat ggaagaagtg cactagatgc tgtggaactt actaatgtgg
     7621 gagctaatta tttgagagaa catgttactt ctgatgtaag gattcattat acaattacag
     7681 atggaggagt agcacccaac atagttcctg ataaagcatc agtatggtat tatacaagag
     7741 ctttaagtag agaagctgta gaaagtacat atgaaagact tgtaaaggta gcaaaaggag
     7801 ctgcaatgat gactgaaact gaagttgaag tagaattttt aggaggatgt tataatactt
     7861 taaacaatca tgttttagca aatcttgtat cagaatgtat gaatgagatt aaacaagagc
     7921 cttggactcg agaggaattg gattttgcag ctgaattaga tagacaaagc ccacaacaat
     7981 ataaggctat gataagtaaa tataatttgc cagaaggaac tcatctatat tatggtggag
     8041 gaaatgttac aaactttaat agttatggct ctactgatat tggagatgta atgcatatag
     8101 ttccgacagc atactttttt acagggtgta cgaatctagg tgcaccaggt catagctggc
     8161 agtttacatc ttgtgcagga agttctatag gagaaaaagg tatgatatat gcatcaaaaa
     8221 ttatggctat gtttggagct aaaatattaa ataatccaga gatagctaaa aaggcaaaag
     8281 aagaatttga taaatctatg aatggtaaaa catataaatg tcctattcca gatgatattc
     8341 caactcctta aaaagacatg aagtttaata tataaaatta taatttttat atcagcatca
     8401 tttcattact aaaagaaagt gagattttgc tgactatctg attaggagga aggaatatga
     8461 aaaatttttt taaagaagcg cagtcaatag aaggggaaat tattagatgg agaaggtatt
     8521 tacatgaaaa tcccgaaatt ggttgcaatc taccgaaaac agtaaccttt gttaccagta
     8581 ctttagaaaa aatgggatat tctccgaaat tgacgtctaa taatggaata gtagctgttt
     8641 tagacagtga aaatggtgga aagaccttat tattaagggc agatatggat gcacttccta
     8701 ttatggagga gacggattta ccttttaaaa gtaagaataa agatgcatct catagttgtg
     8761 gacacgataa tcacacagca atgctcttag gagcagctaa aatacttatg gataataagc
     8821 acttacttaa aggtagagta aagtttgtat ttgaaccaga tgaagagact accagaggtg
     8881 caaaatcaat gattgatgat ggtatattag aaaatcctca ggtcgatgca gctatggcat
     8941 ttcacagtat ggtgggtaaa tttcttaaca caggtcaagt agcttattca cgtggtccag
     9001 ctatggcatc tgcggatata tttgaaataa cagtagaagg aaaagcaact catggagcaa
     9061 gccctgaatt tggagtggac ccaataaaca taatgtcgca tatacaaata gcattaaaca
     9121 ctatactttc aagagaaaaa cctcagaaag aacctgtagt tttaactatt ggtatgttta
     9181 atgcaggaaa cgtaccaaat gctataccaa ataaagcatc tatgtcagga actttaagaa
     9241 catttgacca atttgttaga gaaaaattaa aaaatagaat tattgaaata tctacaggaa
     9301 tagctaaatc atttggagga aatgctaaag tggaatttaa aatggggact cctgcagtta
     9361 taaatgattt ggatgtagga gatgagattg tatcttatgt taaagaagtt ataggaaatg
     9421 aaaatgtaat agactttcca tctgcaatgg gttcagaaga tttttcagag attttactaa
     9481 gggttccagg agtattttta tggattggaa tgggaagtat tagtcaggga tataattttg
     9541 gtatgcataa tcctaaagca atttttaatg aagagggatt agtttatggt tcagctttat
     9601 ttgctcattg tgcagtaaaa tggttagaaa ataattttag tagataataa cataaaatat
     9661 tattaaatat cataaaaggt atatcttaaa aagatgcaaa gatatacctt ttagaaagtc
     9721 atatataatt ttacttaata ttagatgata aaatattttt atgcattttg gattttaata
     9781 tttattttct gatataaata atttgtatac cctttcaggt ctaccttttg ttgagttttg
     9841 tttttcatat aatatagtag cgtttccaga ctttaagaga ctacttagaa atctattagc
     9901 agttctaact gttacattta aacatgaaga taattcctta ggagtaactt catctctttg
     9961 tagaatttca gtaacagata taatcttttg aatagtaaga gtagacaatt tagaactttt
    10021 ggcaattgta tttatatgtg gagttacttt atttgaaaca gtaagtaact tatcacacag
    10081 taaaggtcct attgtctcat aattttcgtt aactaagaaa ctgtaattta aaggatttat
    10141 tttagattcc ttattagcac ttatggcact agacatagct tgctttatat cattaccaat
    10201 tccatatcca acacatactt taatatttgt actattttca atagattctt ttatataaca
    10261 tgcagtaaaa tttttagtta atgatttaat cgtttcacat ttagtgaaaa ttttaaaaga
    10321 attactattt ttttcaatta taaaatcggt taatttatct tttttaatac tatttaatat
    10381 tttataaact tggtctagag tagagtcatc ataatttgta tctttaactg ttatatatat
    10441 tacacttggt tgatttccct tcattctatg taaatgaata tcttttatta agttattaaa
    10501 agtatttttt aataaatcta tatcagggta tatgaaatga aagtttactc ctgcattttg
    10561 taacatagga acaatactac taaaacgagt agtagaaaaa tcgattttcc cttcattcca
    10621 tagattaata tgtttttttg caatcttttt ttcaagtttt gctatgtcag ataaactcat
    10681 tttagatgca ttttcatcaa tactatccat taagtctcca aatgtgccat ttgataaata
    10741 atatgatata tcttttggat tatctgccca gtctgttaag tcaaagtatg ctctggaaaa
    10801 atctaatgta ttatatttaa agataagctt tagaaacata tcatagtaac tttgtaagtc
    10861 agtaccaaaa tcaactagag gtttttttat agttccagcc ttttttgtaa tagctttttt
    10921 ggggataggg ccactaactg caaatccatc aaacctatct tctatttcta aatataaatc
    10981 tccaacatga gaaaaatctc tatagatata tattttaatt tctgcattaa tatttaactt
    11041 ttttatagaa ttatcta
//
LOCUS       DAKBNN010000133        11047 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000133 DAKBNN010000000
VERSION     DAKBNN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.134"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(91..1383)
                     /gene="lysA"
                     /locus_tag="OUB02_002733"
     CDS             complement(91..1383)
                     /gene="lysA"
                     /locus_tag="OUB02_002733"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCT8886477.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(1401..2594)
                     /locus_tag="OUB02_002734"
     CDS             complement(1401..2594)
                     /locus_tag="OUB02_002734"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCT8886478.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(2955..3572)
                     /locus_tag="OUB02_002735"
     CDS             complement(2955..3572)
                     /locus_tag="OUB02_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886479.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(3742..5442)
                     /locus_tag="OUB02_002736"
     CDS             complement(3742..5442)
                     /locus_tag="OUB02_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886480.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(5839..6402)
                     /locus_tag="OUB02_002737"
     CDS             complement(5839..6402)
                     /locus_tag="OUB02_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886481.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(6561..7274)
                     /locus_tag="OUB02_002738"
     CDS             complement(6561..7274)
                     /locus_tag="OUB02_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HCT8886482.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            7566..9230
                     /locus_tag="OUB02_002739"
     CDS             7566..9230
                     /locus_tag="OUB02_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HCT8886483.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(9337..10236)
                     /locus_tag="OUB02_002740"
     CDS             complement(9337..10236)
                     /locus_tag="OUB02_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCT8886484.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(10263..10901)
                     /locus_tag="OUB02_002741"
     CDS             complement(10263..10901)
                     /locus_tag="OUB02_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HCT8886485.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         3629 a         1896 c         1214 g         4308 t
ORIGIN      
        1 taaaccaaaa attaatttag agatacatat cctagcaaat ctgctcataa taaactttaa
       61 tatttaattt ttattatata cttctccatt ttatatctct aaagctaata agtcctcata
      121 gctttgtctc ttacaaatta tcttatcatt tccatcaaat actgatacta ctgctgctct
      181 tggtatttta ttataatttg aagacattga atatccatat gcaccagttg atgttgttat
      241 aagtatatct ccacttttaa tatccattat gtttatatct ccaattaata tatctccact
      301 ctcacaacat tttccagcta ttgttacatg attcatagcc tcatgattaa ttttattcac
      361 aatactacac tcataactag cttggtacaa cgaaggtctt acattatctg tcatacctcc
      421 atctacactt acataaaccc taacatcttt tatatctttt atagaaccta ctgtatacaa
      481 tgtacttcct gcatttgcaa ctattgatct tcctggttct attaccaatg taggtacttc
      541 tattccaagc tctttacatg aagtttctgc tttagtgatt atagtttcac aaaactcttt
      601 tatagtttta ggtttatctc cttcgctata ataaacgcca actcctccac ctaggtcaat
      661 ttctgttagt tgtatgttga atttttcttt tatttctttt accaagttca tcattatatc
      721 cactgtatct atgtatggtt ctacatcaaa tatttgtgaa cctatatgag cgtgaagacc
      781 cacaagttta atatttttat attcacttaa tttttctatt gctctaaata aatctccatt
      841 tactaaagca aatccaaatt tagaatctat ctgtccagtt tttatataat catgtgtatg
      901 agcttctata ccaggagtta ttctaaagta tatctcttga gttttacctt tatcttcaca
      961 taatgcttca agcaaatcta attcataaaa attatccacc acaaatttac ctacacctaa
     1021 atctaatccc atgtttattt cttctttagt cttattgttt ccatgaaaga aaactttttc
     1081 cataggaaaa tttgatttat aagctgtata taactcgcct cctgaaacta catctaaaca
     1141 catttgttct tcatttataa gattacacat gtaaagaggt aaaaaagctt tccctgcata
     1201 agcaacctta ttttttccct ctctagcctt aaaatattct atatattctc tacaattttg
     1261 tctaactaaa gcttcatcaa atacataaag tggagtttga tattttttac taagttctaa
     1321 acaactcact cctccaatat ataattcatc attatttaca gtcatagttc catgaagttt
     1381 cataatagcc tccccaaatt ttatatatta aatacttctg ctattttttc tatagctact
     1441 tgcttatcat cagaatttat agcacaagtt atacgaatct ctgatgtagt tataagtttt
     1501 atcattatac cattttcatt gaatatctta aatactttag cagcaactcc agaagtattt
     1561 ttcataccaa gacctactaa agaaaattta gtgatattat tatcaatcac tacatgttct
     1621 tcatctgtat atttagatac tatcttttta cattctccca actcttcttt tggaactgta
     1681 aatgatacac ttatcttatc taaaataggt gcagtttggc ttatcatatc tacacttata
     1741 cctatagttg ctatatcttc aaataagcta gatatatttt cagctttaaa atcttttatt
     1801 gttatagaac tgtcatcatc actagtagct aatcctgtta ttactttatc ctctaatttc
     1861 atgtcttttc cccctcttat atatgttcct ttttctgtac cacatgtacg tccgacatag
     1921 atttcaacac catatttttg tgcaagctct atacttcttg aatgcattac ttgtgctcct
     1981 aaactcgcta actcaagcat ttcttcatat tcaatctcat caagtttact agcctttgaa
     2041 tattttcttg ggtctgtaaa ataaattcca tcaacatctg tatatatctc acattttcca
     2101 tttaatttaa ccgcaagagc tacagcagat gtatctgaac cacctcttcc aagagtggtt
     2161 acatcaccat cttcgttgat tccctgaaat ccagttacta tcacgacatt tccttcatct
     2221 aaactatttt taattctgtt tacgtttata tcatcaattt gagactttcc atgaagacca
     2281 ctcgttttta tatttaactg ataagcatta taacttatag ctttacatcc aagtgcattt
     2341 aatgctattg ataataaaga tgctgaaatc atttcaccag tagacattaa agcatctaat
     2401 tccctcttac taggttcatt actcaattcc tttgctaaag ttatatattc atcagtggat
     2461 tttcccatgg cagacacaac tattaccatt tgtggatttt cttttcttcg ttctattata
     2521 ttttctgcta ttgattttat tttttcagta tctgctactg aacttccacc atatttttga
     2581 acaattatac tcatgctttc tccccctcaa gctaaatttt gataaataaa aacgcccaaa
     2641 ggtatacttt ggacgtgtaa atttcataga ccaagtaacc ttgtagctca ccactttaaa
     2701 agtgacagcc ttatgcatat tctgcataag tccagaaaat ttgtccgtca acaaaatttc
     2761 ttcggctata taccctttca tcaattatca acgcttacca gctcctattg aatactattg
     2821 aatacagcta cctctaccct aggctcatta tttttaatta atttattaat atagttttat
     2881 catattctat ataatattgc aacaattatt ttacaaaatg tggcaaatcg atagttttct
     2941 tttctaaagt tagcttatat acaattaaat agtttattta atcctaaatc tataaaattt
     3001 atgctatact cattatcata tctctctaaa tcatttaatt cggatagatt atctggtgta
     3061 actaccaaaa cgtttggtac tttaaaaaat ggaattgtag atttatctat ttttttagaa
     3121 agattttcgt atttagaaat gtctatttca ttatttaagt caacatcaac taaaacttgc
     3181 tttatattac caaattggtc tgtatattca ataaataaat cgtatttcac atcatctaca
     3241 ctaacatttc ggtaaaatct ttttacatca caacctgcag tattgattct aacaactaat
     3301 tctgatccta ctagagcctt tattaaatca tctcccttaa aaagtttact accgtttaaa
     3361 tagtatacat aagagaggtc tttataatta taataatcca catcacaagg atattttttt
     3421 agtgtatatt cactacacat ttttctcaaa gtttttttaa aatttttttt attgtatctt
     3481 ttttggtctc cataaatttt tctaaattga gactctgtaa cacatctaac cctgctaatt
     3541 aaaagtaata tctctttttc catattcgtc atagtacatt ctccttgaag tattatttgc
     3601 aaagttgtac taatattata ttaattttat gacattaaat ttacaaagaa ttaaaataaa
     3661 atagctaatt tttatagaat taaattctat aaaaattagc tatcactatg ttaaatacta
     3721 ttatcaatct ttttatttaa attatacatt accttgattt tcagaatctt tggaatctgt
     3781 attttcatca gttcctgttt tttcttttgc ttttttatct tcaaagtttg caatatttag
     3841 gttatcaaat tctgacattt ctattacacc agattcttct tttgaatacg atattttgac
     3901 tttatcacca acctgagtca atggcaattc gctagatact tttgaagtag ccctaaatat
     3961 tatatcttta tttgaatcta aagttacata ataattagtg ttaccacctc gcacatctgg
     4021 gcttattctt gtcactgtgc cttctaatac ctcatctttt aaatcattat caagttttaa
     4081 atcattaccc ttagattcta atgcatcctt atagtttctt aatgcttcat tcttatcttc
     4141 acctattcca agtacattat aatcttcaac agatacaaat gcaactctct taacaagacc
     4201 agccttatct tttaatgatg atacatatgt tggctgaccc aatatattat acattacagg
     4261 gaacgtcgct tcataatttt tttcttgaac attaccttca gctgatttca ttgcagccgt
     4321 ttcagtagct cctggttgtt tatataatct agcttctttt gttctagaat caactaacat
     4381 aaatccaatt gtagattcat ctccacctga agatgttata cctgtatacc aataagacct
     4441 gttatcattg ccataaacta atgatgtacc ttctgttggt actaaaactc ccttttctga
     4501 tataacagag tttaaaaatc cattaacata aacaccccag tctttaatct ggtctgttac
     4561 aaagctttgt ggttgtattc tatccaccca tttaggtgtg tttttaacat cataaacatt
     4621 tatttttcca gtttctgcat caacagtggc tattcctgtt gcatttgctc ctccatagcc
     4681 aattttatgt tcatataaac ttacaaccca ataaggtctt ccttcatcat ttatttcaag
     4741 agtgaaatct gtcataccaa catttacaat accatgtatg tataaatgtc tttgtaaatc
     4801 ctgatgtaag taggcttctg gttggtaaac tatctttatt ggctttccat ctatttcttg
     4861 aactaattgg acgtcttgag gattactagc tgatactttt acataaccac ttgtaccact
     4921 tagagatgtt atccatttaa ttatgtctct gtgaacaaga ggcgctaccc agtacaactc
     4981 tccatctaca ttttgtatat gaaattttcc taatttggat acactcccta ttgcaggaac
     5041 ttctccaagc tttttgtcac ctaatttcat tgccatatct tcatctacaa gacgaatgtc
     5101 atttacactg acagggctaa tgtcatcagt aaatttactt tcagttactt ttcccaatag
     5161 ttctctatat gattttgcat gaagtacagg tgtagaagtt ataaagggaa ctactacaat
     5221 atacacaagc aaagctattg ctattgaaaa attatattta gccattttag atgctctttc
     5281 atttctatct aacatcatat ctagtattcc tgctacaaca aagaaaatta ttcctactga
     5341 aaataaagat gtaaaatcta atcttagtac aggtaattta ataaatgcat aaattattac
     5401 tattattaat gcaactccat atgttttaag atatcgtttc atttaatact cctctctaaa
     5461 aaatgttatt attactatta tatccatata tttacaaaaa ataaatattt atcatattta
     5521 ttatatatta agatatcttt atatatttat agttctgctt attcaactat atttaacatt
     5581 atacgtgtat gtataaatac tttttatata ctgtttgtgc tatccatccc gcacctaaag
     5641 gagtgggatt tttgcactat tctgtattat atatatccaa aaatattata aaaatacaaa
     5701 tattagcaga aatttaaaaa cttaagaaag tattctaact aagatttaca aattcttaat
     5761 cctatcgaag ttaaactact atcttcagcc taattatcta gcaatatttt tttatatgaa
     5821 aaactccaaa tcaagttttt aaatgtttgc gatttttgat acataagaat gttgtctaga
     5881 attatattct gataataaac cttcatactc taaggttaag ccagataaat tataaaaatc
     5941 atttggcgtt acttcaaatg cttgtttatt taaaatgtct atctcacttt tttctaacat
     6001 gtcagcaaca aaatttgaac atacatattt atattcagat tgtctctgaa tattcattag
     6061 taaatatatt aatgccattg tatcgtaata gtaatcttct ctataatcac taattaattt
     6121 tatattttta tatagatttt catattgtaa attatctact tctaacgaat ataccctaca
     6181 cacagtgttt tttcttatag catataatcc ttgatttata ttttcttcaa caaatcctcc
     6241 aataaatgga ttccttggat ttagccttcc aaaagaatac attggtttta gattttcatt
     6301 taatgatatt gacacatgtg tatacagctt tttacttata ttccttatta agtaagataa
     6361 tatagttcct gtatatgttg ttacaatata gactttattc atcatttccc cccaatctag
     6421 taactaaata atattataca cttacaattt aatatcataa gatattctaa cataataaat
     6481 gtggttttac aaattttgat tttaaggtac tttaatatta tataatataa taaaaatata
     6541 aatataagtt ttctatattt ttaaccaaca gatgttttct tcaaattaat cagatgattt
     6601 agtatatctt gaacctttcc agagtccttt aaatatccta atgccccatt tatttgtgat
     6661 tttaaatcta tttctacttc catatccaaa tcaacatttc ctttttttat agatatattt
     6721 ttaagtgtta ttggataaag ataacttttt tccaaagtaa tcttatttcc attaacctca
     6781 aatggaactt ttttattgta gttacttaat attttttcta gcattttatc acttagttta
     6841 aattttccaa ttttcacatt ctcaagactt acaactaagt ttccatcctt taaagtaggt
     6901 ctcaattcaa gttcatattg actatttacc aaaccaaata ccttatatgg tcctgctacc
     6961 tttattttac catttttcat ttcaacaaaa ttattttcaa gttcattaat tccatgcttc
     7021 tgcatcaatg tatatattaa atttttaaac tcttcctctg aaacagatat ttttccaacc
     7081 attctaagtg gattttttac tacctccata gaatttatat atgagccttt tgataaatat
     7141 tcttcatcta tgattttatt attttgtgaa ctaacatcat atctttcttt tggtgtcaag
     7201 atatatacaa caattcctgc aagtactaaa acaataacaa ttaaacttaa caaaatcttt
     7261 gcaacctttc ccataatcgt ctcctttaca aatctgtcat cagatttaaa ataaatttag
     7321 ataactaact tcacataata ttcttaacaa tatacatact ataatacagt cttttaaaaa
     7381 ttaattcaac caatctaatg ttttgtaaat aaactgtaaa aacatgtttt tagtataaaa
     7441 gttaatattt tgcataatac tcttaattac acctttattt ttttctaatc ttggattaaa
     7501 attaatatta tgcaatacag tgtagtgttc tgtattataa tcaaattaaa taggaggtat
     7561 agaatatgtc aaatgaaacg aactcatcta actttataaa gaatattatt ataaacgact
     7621 tagaaacagg aaaacatgac agtatcataa ctcgttttcc acctgagcca aacggatatt
     7681 tacatattgg tcatgcaaaa agtatatgtc ttaattttgg attagctaag gagtttaatg
     7741 gaaaagctaa tttaagattt gatgatacaa acccattaaa agaggatgtt gaatatgtag
     7801 agtccataaa agaagatgta aagtggttag gatttgattg gaacgagtta aattttgcat
     7861 ctaactactt tgatgaaatg tataagagag ctttaatact aataaaaaag ggtaaagctt
     7921 atgtatgtga tttgacacaa gaggaaatga gagaatatcg tggtactcta actgaaccag
     7981 gaaaagaaag tcctcataga aacagaacta ttgaagaaaa ccttgattta tttgaaagaa
     8041 tgaaaaatgg tgaatttaaa gatggtgaaa aaacattaag agccaaaata gatatgtctt
     8101 ctccaaatat aaaccttaga gacccaatta tatatagaat atctcattca acacaccata
     8161 atacaggaga taaatggtgc atatatccta tgtatgcttt tgctcatcca attgaagatg
     8221 caattgaagg aataactcac tctatatgta ctttagagtt tgaagaccaa agacctcttt
     8281 atgattggtt tgttaaagaa tgtgagatgg aaaatatacc tagacaaata gaatttgcta
     8341 gacttaatat aaataatact gtcatgagta aaaggaaatt aaaacaactt gtagatgagg
     8401 gtattgttga tggttgggat gaccctcgtg ttcctacaat atccggaatc agacgtaagg
     8461 gatatactgc agaagcattg cgcaatttct gtagtgaaat aggggtatca aaagttaact
     8521 ctacggtaga tagtcaaatg cttgattatt tcctaagaga aaacttacaa ccaaaggctc
     8581 ctcttactat gggagtttta agacctttaa aacttattat aactaattac ccagaagata
     8641 agattgagat gcttgaaata gagaataatg ctaaagatga atctcaagga aagagattag
     8701 ttcctttctc tagagaattg tatatagaac aagatgattt tatggaagaa cctgttaaga
     8761 aatattttag atttttccca ggaaatgaag tccgtttaaa aggtgcttat tttgtaaaat
     8821 gtactgatgt aattaaggat gaaaatggaa atgtagttga aatacatggt acttatgacc
     8881 ctgaaactaa gagtggctct ggctttacag gacgtaaggt aaaatctaca atacattggg
     8941 ttgatgctaa atcagctata ccatgtgaat ttagattatt tgaaccatta attcttgatg
     9001 atatacctga aaatgaaggt aaacatttct tagaacaaat aaatcctaat tcattggaaa
     9061 tattacaagg ttttgttgaa cctacacaga taaaagatgc taaacctttt gataaattcc
     9121 agtttgttag aaatggattc tttagtatag ataataaata tacaactgat gaaaaatttg
     9181 tatttaatag aatagttcct ctaaaaagtt cttttaaacc aggtaaatag tcttacaatc
     9241 aaaaaatgaa gacttaaaat ataaattact tctactataa ataaacttag ttagaagtaa
     9301 ttttttattt gagtcttcat catttacttt aaatgattaa cttttttcac tacagattcc
     9361 catgacttct attccatctt ttatgcattc tatgttaaga ggaaaatctg gtcctttttc
     9421 tccgtcaata tctgttataa gtgaatcttc acattctata tgcacctttt tagttttaaa
     9481 gtatagtatt tcatttccat tatattgatc taaataatca cctctaagca cacttatcaa
     9541 aactggtatt gattttggta ttggcattcc tttaaaaata attacatcca aatatccatc
     9601 atctatttca gccttatatg ctagatttat atttcctgct gttttgccat taaaaatcaa
     9661 cataagatac atatctccat cataatacat ttcctcagaa gatactttta tcttaaattt
     9721 cctcatatac aatgcttctt ctattccctt tatataataa gaaattcttc caaatgaatt
     9781 ttttaagtct gtgtttatcc tttgtgatac atccgtaaac ataccagcac ttgcaacatt
     9841 tataaagtac ttattattga ctttccctat gtctattttc tttggttttg aatttatgat
     9901 ttcatttata gcttctttta tatcaaatga aatttgaaga gcatttgaaa aatcatttgc
     9961 agttcctgta ggcaaaattc caattggaat ttttatgtct aattctttca ttgcatttac
    10021 tactaaatct acagtaccat caccaccagc tatcaatatg tgttcataag aactatcaac
    10081 ttccttaaat gcattcttta caggttcttt aatagttaat ctatagggta tcaataaata
    10141 atttttttct tgatataatt ctatgatagt gtctaaattt gataatatct ttttttcacc
    10201 tgaattagga ttgtatataa gttttacttt tttcattatt tctccttact cattttattc
    10261 ttttagtatc caaaatcttc atttaaaaaa atcacatgta ttctttctgg tgttaattgt
    10321 ctttttataa gtgtataact acagcatatt cttcctggac ttgaacagtc tatacatata
    10381 ccagtagtct tacatccagt atttgtactt aatcttttac aattggctgg agcagctatt
    10441 gtttttgttc tttttatagc ttcatctaca tctttaacta ttttatttac tcctgctata
    10501 actattactt tatcaggtcc atatatcata gcagcaaccc tattcccatt cccatctaaa
    10561 ttgtaaagtt ctccatcctc tgttatcgca ttagtacttg taaaataagc atctgcagaa
    10621 aatgactttc tatatatttc tttcatttct tctggtttga tatcttttgc atatctatca
    10681 agaaaattat atttttctct tataaaatca atcactcctg tttcaaataa ggtcatagaa
    10741 cctccacatc ctactagaga acctgcttta actatttctt caattttttt aagaagctct
    10801 tctttgttac ttactaaata ccctttcata ttattgtttt ccaaagattt tattgttttt
    10861 tctattcttt tttcattaag ccaatttaaa ttttgatcca ttttaacttc ccccttattt
    10921 ttaatatgta cttaattatt tataaacttt attttcttta taagtttatt ttagtacatc
    10981 taaaaaaaaa tacaatataa aacttaaata gttttacata attttttcca aaataactta
    11041 ctcaatc
//
LOCUS       DAKBNN010000134        10861 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000134 DAKBNN010000000
VERSION     DAKBNN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.135"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            69..1193
                     /locus_tag="OUB02_002742"
     CDS             69..1193
                     /locus_tag="OUB02_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT8886486.1"
                     /translation="MTFLFVKYIIKQSTVLSIVSTLLVYLIEQLVYGFTAPLNYLIFT
                     SDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLII
                     LFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQ
                     ALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKA
                     QDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDF
                     DLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTRGSGI
                     GLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1232..1717
                     /locus_tag="OUB02_002743"
     CDS             1232..1717
                     /locus_tag="OUB02_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886487.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QRKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1980..2216)
                     /locus_tag="OUB02_002744"
     CDS             complement(1980..2216)
                     /locus_tag="OUB02_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886488.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
     gene            complement(2371..2595)
                     /locus_tag="OUB02_002745"
     CDS             complement(2371..2595)
                     /locus_tag="OUB02_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCT8886489.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2692..3945)
                     /locus_tag="OUB02_002746"
     CDS             complement(2692..3945)
                     /locus_tag="OUB02_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HCT8886490.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLNNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4205..5194
                     /locus_tag="OUB02_002747"
     CDS             4205..5194
                     /locus_tag="OUB02_002747"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCT8886491.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5266..6006
                     /locus_tag="OUB02_002748"
     CDS             5266..6006
                     /locus_tag="OUB02_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HCT8886492.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6222..7373
                     /locus_tag="OUB02_002749"
     CDS             6222..7373
                     /locus_tag="OUB02_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HCT8886493.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7398..8153
                     /locus_tag="OUB02_002750"
     CDS             7398..8153
                     /locus_tag="OUB02_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HCT8886494.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNNNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8345..10732
                     /locus_tag="OUB02_002751"
     CDS             8345..10732
                     /locus_tag="OUB02_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HCT8886495.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNSNI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         4348 a         1208 c         1771 g         3534 t
ORIGIN      
        1 ttttaatcat tataaataca tattttaatt tatataattt attttcttca cagattctat
       61 tttttcttat gactttttta tttgtaaagt acataataaa acaatctact gttttatcta
      121 tagtatcaac tttgttagta tatttaattg aacaattagt atatggattt acagctccat
      181 taaattactt gatatttact agtgatataa atacagatat ttataaattt gatataaata
      241 tgggactatc ttctttaatt actattataa tagctggatt tatatattgg tatagttcta
      301 aaaagtttaa tattaaggtc atgaactttg ataaatatat agcaattttg atgattccat
      361 tgttgcttat aatattgttt atgcaatcat ttgaatatag ttcaaatata aatatagata
      421 caagtcttgg gattgtaaag ctgctattaa atacttctat aactgaagaa attcaagctt
      481 ttcttttttc tataattgga actatatgtg tatttttttc tctatttacc tttaagaaac
      541 taattcaagc tctcgaagat gacaaggaga gggcaattat gaatcaacag attcatgctc
      601 aaaaaaacta tattgaagaa gcgaaatcac gattatcaca aactatatcc tttagacatg
      661 attttaataa tcatttagct attgtcaatg gattgttgaa aaaagaccaa attttaaaag
      721 cacaagatta tttaaataag cttgaaaaag taacaagtga cctatcattt tcttgtaata
      781 cacagaatat tgttattgat gctcttttca acaataaatt aagtatagca aaacaattag
      841 gcatagaggt agattgtgaa gtaattatac cccaaagcac taacatgaac gattttgacc
      901 tatgtatagt gtttgctaat gctattgaca atgcaattaa agcttgtaga gttgtagata
      961 aacaaaatag gtatttaaag ctttccacta agttagaagg tggatttttt atgatagaaa
     1021 taaaaaatag ctataatcca ataaataatt atacacgtgg ttcaggtata ggtcttttaa
     1081 atataaaaga aattgctaaa aaatatcaag gggctataag tatagataag acatcagatt
     1141 attttcaaat aaatgtattg ctggttattt cattacattg cgctagtatt tgagaacaat
     1201 ttcattgaaa atagtatttg taagctatat tatgtatttg aatagagaaa gtttgatata
     1261 tagaaatgag gaggttacta aaatgaaagt tggaaatata aatggtcaag caccaattac
     1321 aaatggttta gatagttgtc aaagtgaggt acaaggctta gaacaaagga aaaaatcatt
     1381 acaggatgaa atagagaaag taagaagtga taaaagtatt gatggtgaaa cagccaaatt
     1441 aaaaatagaa gctttaaaaa aagaaatgga agagattgaa aataaaattc aagagttaaa
     1501 aggaaataaa gcaaaaaaaa caaataaaga agaaaataat gtttcagaag taaataataa
     1561 tctattagag agaaaaaaag aaccttctaa taatgaaaat gtaaaacaac tatatgatgt
     1621 ttttgaaaga agcaatgtag acgaacaatg ggataatact tatcgtgttc tccaagatga
     1681 taaaaactta aaagtaaaat ttaatagacc tatatagttt atttataaaa atagttaata
     1741 ttttaattat caccactttt tagaagaata cacactttat tttaaagacc cacatattta
     1801 tttacagtta gcacatttat atgtggtttt ataactatta tttacaagtc tatattgata
     1861 acttttacat agtatataat gtttaaatac ttattcaatt ttatctatac ataaacaaaa
     1921 aaagagattt taaaatctct ttttagattg ttgacttatg gctttttgat ttaataaaat
     1981 caagaagccg tttgaatttt atatgttggt tgataccctt ctgatattga aacaccattt
     2041 ttgtttccaa aatcgtttaa aattaagaac ttattatgaa tgactatttc attatgaaca
     2101 ataagcagat aacatgaatc ttcaatcaat gttttaacag tagcattaaa tgcttgctta
     2161 tacagttcta tcgtttgttt gaactgtcca ttaaagtaaa tatgagtcaa taacataact
     2221 atatctacat aaattaatat aaatttttta tactgtacat tatctaactg ttgattatct
     2281 gtattacaac atgattgaat ttaaaaagcc atcaacacat agaatgttga tggcctgaaa
     2341 atagatttaa aatctctttt ttattatata ttaattattt tttaaattta tattttttgc
     2401 atcagatttt ctaatagcta agtcaaaccc tctaaatcta atcactatag ggtcattaag
     2461 aggagctatc tttttcactt caacttcagt gccatctatt agtcctaagg ctagaagtct
     2521 cttcataagt ttttcatttc cagctatatt gtctataatt cccttttgtc caagttttaa
     2581 gttgtaaaca gtcattatta acacctccta atgaaaatca ttatcaaact ttatatttat
     2641 aatatataac aaaaagagca aattgtcaat atataacaaa aaataaacat actaagttag
     2701 tatgtttata aaaaatagta tacatgggat actaatagca tctagcaaaa aaccacttac
     2761 taatggtaca accaaaaagg cttttggaga atcaccatat ttttgtgtca aagaactcat
     2821 atttgccaaa gcgttcggag tagctcctat tccatgtcca atcaaaccac ttattatgat
     2881 ggcagaatca aaatcttttc caagtactct aaagcaaata aatattgaaa ataggattat
     2941 aaagattact tgaaaagtta ctattaagag aataggaggt aataatgtag ataattcaaa
     3001 taaatctata ttcataagtg ataaagttaa aaaaattccc aatgaaagtt cttttaaaaa
     3061 atctataagt ttaaaattta attctagtat atttatctta tcatttaaat ttctaaaaat
     3121 aacagccgaa aacatacaag ttacaacaga gggtaatagt atattaaacg ttatatagaa
     3181 gcatctactt ataatttctc ctgtatttat acaaataaat atgagaagaa cctgttccaa
     3241 aaatacagat ggtgttattg tttcggagaa gttattattt tttatcctat tcttatttct
     3301 aaaaaattta cttccaaatc tctttagatt tctattatat ctaggaatcg acaagtctat
     3361 atttgatgat ggttttaatt tatatttatt tattagaaat ttagctacag gacatcccaa
     3421 tataccaccc ataataagtc ctattgttgc agcagataat ccaaccccaa ctgctccttc
     3481 tataccgaga ttttctattg taagtccaaa tgcagcagaa taaccatgac caccttccat
     3541 agatacattt ccacacataa gccctaaaag aggttcaagt tttataatct ttgatattgt
     3601 aatagctaat atattttgac aaaatgccaa tactccacac aaaatccaat atctaaataa
     3661 tacactaccc cctttcttta taagatttat gcttatttcg agtcctatag ttgtaaaaaa
     3721 gaaagttata aaatttggca ttaaatttcc acttatagat atatctgcta tattaaaata
     3781 tttaaaaaat aaatttagaa aacaaaataa tagacctcct attactggtg caggtataca
     3841 gaaattattt aaaaagttaa ctttattatt caataaatta ccaattaaaa agaagaatat
     3901 agatagtgtc aaggtttgca ctatatcaaa acttaaggta atcaaaataa gtcctccttt
     3961 gtaattcaaa gttttaatta tataacttaa attcttatgt attgaaattt tattatcaaa
     4021 tgtatattaa taaatgtata ttaaaaagaa tcagtagata taattagata gtcataaatt
     4081 aaattaaaat attttaatga gatatgactt ttttagaatt aaaaaccgta gtatattgat
     4141 ataatagaaa taagacttaa aaatctgttg atagtattat ttataagtac agtaggagga
     4201 taaaatgttt gaaaatttac ctaagataga tttacattgc cacttagatg gaagtgttag
     4261 agtagaaacc atgcttgata tagctattaa agagaaaata gatttacctt caaataatat
     4321 ggatgaaata aaaaaattag caaaggtttc ttttaattgt acttcactag atgaatattt
     4381 agaaaaattt gatttaccct taaaagtaat gcaatctaag gaaaatttaa aaagaattac
     4441 ttttgaactt ttggaagatg caagtagaga aaatgtaaaa tatattgaaa ttagatttgc
     4501 accattactt cacacccaaa aaggtatgag tgttaaaaat ataattgagg gtataattga
     4561 aggaattaga gaagctgaaa gtatttatga tataaaaggc aatttaatat taggttgtat
     4621 gagaacaatg acctcaaaag aggcattatt agtaattgaa gaagggaaat catttgtaaa
     4681 taaaggtgtt gtagcagtag acttgtgtgg tccagagaaa gagggatttt gtaaggaata
     4741 taaagatgtt tttaaattag caagagaata tggatataaa gttacaatac atgcaggaga
     4801 agctgcaagt ggagaaaatg tattggatgc tataaatatt ttaaaggcgg ataggattgg
     4861 tcatggggta aaaattaaag accataaaaa ggcgtataat ttagttaaag ataaaaaaat
     4921 attgcttgaa ttatgtccaa ctagtaatgt acaaactaag acagtagatt cttatgaggt
     4981 acatccattt tatacttttt ataaagataa tttacatgta agtataaata ctgataatag
     5041 aactgtatca gatataaatt taagttcaga actaaatgtt atatttgata cttttaaact
     5101 aggattagaa gactataaaa taatttatag aaatgcagta gaagctagtt ttgcagataa
     5161 agaaactaaa gagtatttga atagtttaat ttaatagata aaaatattta aactttaaat
     5221 taagctatta aattgacaaa aatacattgt attaggagaa aaaatgtgaa agattatgaa
     5281 gatgttgttt atattaaact agtcaaaaaa gaacttgaag aatggaagaa aaatatatta
     5341 aagaaacctt ctatatttga caaagcatca aagggtatac agaataaatt taatggagta
     5401 ttgccagata aatatcatga aataattact tcttctataa aagtaatgac aaaaaccgta
     5461 ctgtttggat ataaatatgc tacaaagcca ccacttagaa atatgtcatt agaagaaaga
     5521 gaaaaattag ttttagaaaa agctaaaact tacaagacta cagcaatgat tgaaggggca
     5581 ggaacagggg caggaggaat cctcttaggt ttatctgatt ttcctttgct tttaggtatt
     5641 aaagtgaaat ttttatatga tgtagcttca atatatggat atgatgttaa agattataga
     5701 gaaaggattt atatattaaa tatattacag ttggcttttt ctagtcaaca gcatataaac
     5761 aaaactttta aattgatgaa taactgggat gaatatgtaa atagtctacc agaagacatc
     5821 aatgattttg attggagaac atttcaacaa gagtataggg attatttaga tttagctaag
     5881 tttttacaac taatgcctgg aataggagca tttgtaggat tttatgtgaa tggaaaatta
     5941 attgataaat tatgtgaaac tgctgtgtct gcttatagat tgaggattcg agagcttaag
     6001 ttataagaaa aattataaca taaaaaatta gttatacaat atatctatat gtatctaatt
     6061 ttgcattgat aaaaacaatt atggataaaa ggtaaagcat gatagaatct aatgtagagg
     6121 tttgtatttt aatggaaaaa tttagttttt attgcttaag atacaaattg aattacgaag
     6181 agattgaatt atgaagagaa gtaattggga ggaatatttt catgacaaat ttgccaaaac
     6241 aatttgatag cttcaatgaa gctagacaaa aaggatttat aaatgcaaag gaacttaagg
     6301 aaagtgggaa aaagatggtt ggagttttct gtacatttac tccagttgaa attccaatgg
     6361 cagctggagc aactgtagta ggtgtatgcg gtgttagtga agagccaata cctgatgcag
     6421 aaagggtgct tccaagaaat ctttgtcctt taattaaatc aagctatgga catgcaataa
     6481 cagatacctg tccttatttc tatttttctg atttgttgat aggagaaacc acttgtgatg
     6541 gtaagaaaaa aatgtatgaa gaacttgcaa aagttaagcc aacatatgta atgcatttgc
     6601 caaatacttc aaaaggagaa tttgcctata aactttggaa agatgaaatg ataagattaa
     6661 aagaagaggt tgagaaatct ttaggtgtaa ctataactga agaagatata agaactgcta
     6721 taaaagataa aaatgaagaa agagaacttt taaaagagtt ttatgcactt ggtaaattac
     6781 aaccatctgc acttacaggc cttgagttac ataatgtttt atatcaagca ggttttaaat
     6841 ttgaccgtgc agaacttaaa cattctttaa gaaaagttat agatgatatg aaagaaagat
     6901 atgaaaaagg tgaatgtcct gttcaaaaag ataagccaag aatattgata acaggttctc
     6961 ctattggtgg aataagcgaa aaaatagtta aaactttaga agatgcaggg gcatcagtcg
     7021 ttgcatatga actttgtgga gctattagat caaacgattt attggttgat gaagaaatag
     7081 aagatgtata tgatgcttta acacaaaaat atataaatat aggttgttct tgtatgatga
     7141 ataatgacaa tcgtattgaa ttattagata gaataataga tgaatataat gtagatgctg
     7201 taatagatgt agttttacaa gcatgtcata catttaatat agaaagttat agaataagag
     7261 aatttgttac aaaagaaaaa aataagccat ttatgtctct tgaaactgat tattcaaaat
     7321 cagatactga acaattaaga actagatttg aagcgtttgt agagatgttg taggaataaa
     7381 taaaagtagg aggaactatg tttagtatag gagttgactc agggtcagtg gcaacaaagg
     7441 gtgtgctatt tgatggtgaa aaaattatta agaaaataat tataccaact ggatggagcc
     7501 ctaaaagtac atctaaacag gtctatgagt tgctaagtag tgaaattgac aaaaaagata
     7561 taaaaaaggt ggtaggtaca ggttatggaa ggggagtaat ggattttgct gataagaaag
     7621 taacagaaat aacttgtcat actagaggta tttacttttt aaacaataac ataaggacta
     7681 ttttagatgt aggtggtcaa gatagtaaag ttataaattt agatagagat ggaaatgtat
     7741 ttaactttat aatgaatgat aagtgtgctg caggaactgg tcgattttta gagattactt
     7801 ctaacctttt aggaagcgat attgaaagta tagatacatt agctaaagga tatgaaccag
     7861 ttaatatatc tagtatgtgt acagtttttg ctgagtctga aattgtaagt cttctagctc
     7921 aaaacatttc tactggagag gtagcagcag ggatacttaa atctattgca aataaatcca
     7981 cctcaatgtt agcaagaggt gaggtaatag atgaagttgc atttactgga gggcttgcaa
     8041 aaagtaaaga actagtaaaa atgatagaag aaatactggg taaaaaaatt tttattgcag
     8101 aagatactca aataatagga gctttaggag ctgctgtaat aggatttaga taaaaacata
     8161 taaaaagaaa atgagaagct gaatttatta taaataaatt cagtttttta ttttatttta
     8221 tttatttata aaggaaaact ttggtataat tgatttaaat gattgtatat ttgtaaatac
     8281 ttgatattta gtcatatata acattataat tttaaaaatt agctcaaaat aagggggaga
     8341 aagcgtgaaa aaatttactt caaaaaaagt aacaaggttt gttgcgctgt ttttaatatc
     8401 tattttagtc atgtcaacaa ttcctgtatc agctgacaat agcaatacat tgaatcagat
     8461 aaaaaaagca gaatacagtg agacgtataa acaatatcta aatgatgcta aaaatggacg
     8521 tacagaaaaa tataatggga ttataccaaa tccatatcag ttggaaggta ctcaaatcca
     8581 aagtaaagga agaacagctc caacatctta tgaccctaga aaattaggtc ttatgacatc
     8641 aataaaaaat caagaggatt taggaatctg ttgggatttt gctgcaatgg caaccttaga
     8701 aagtttctta aagttaaata attatggaga ctatgattta tcagaagaac acttaagatg
     8761 gtgggcttca gatggtgaat atggatggtc tgtaaatgat atgaatggtg ctttaaatta
     8821 tgaagctatg ggatatttaa catcttggtc aggtcctaaa ttagaaaaag atatcccata
     8881 caatggtaga gtatctaaag cacaaggagc aaaaaaacca actaatatga atacagcacc
     8941 tactgttttt aatgtgacag atgctgtgtg tgtaagcaat gacataaatt ctacaaaaaa
     9001 tgctatatta cagtatggag ctgtaacatc tggttattat gaagatatta aatatcaaag
     9061 tgatgaccaa aattcatatt attgtccaac aaaaagtttt aatacaaatc atgctatttc
     9121 aattgtaggt tgggatgata actattctaa agataatttt aattcaaata ttagaccatc
     9181 aaaaaatggt gcttggttaa taaaaaatag ctggggagat tataattctg agggtggata
     9241 tttctggata tcttatgaag acaagacact tatgtctgat atagataatt tttcaatcaa
     9301 aggtggtaaa aaaccaaatg atgatgagaa gatgtatcaa catgactatg caagtattgt
     9361 acctttaatt tcaaaaaaga taacagctgc aaatgtattt gatttcaata gaggcgatga
     9421 aactttaaaa tctgtaatgt tcttaactga aagtatagga gctaagtatg aagtatatta
     9481 tgctccagta gtaaatggta tacctcaaga aaacaatatg aaaaaattaa aagaaggtac
     9541 tgcccaatat tcaggataca taacagttcc aattgattct tttgatatac cagaaggaaa
     9601 gggtgcaatt gtagtcagta tagaggggaa aaatggagag tcaactatag gttctgagtc
     9661 aaatgtacca ggatatgata catttaaagc aaaagctaat ttaggtgaaa gttatatcat
     9721 agataatact ggaaaattct ttgatataaa tagagatagt aacttctatc cgtgtaattt
     9781 tactataaaa gctgttacag aaaagtcttc tggtgagagt atcccaaatg agtctttaat
     9841 tggtagtgat agatatgaaa ctgctatcaa agttagtcaa aatggtttca attcttcaga
     9901 aaatgtagtt ttagtaaatg gcagttcaat agtagatgca ttggcagcaa ctccttttac
     9961 atctgctata aattcaccaa tattattgac acaaaaagaa gctttaaatt caaaaactaa
    10021 agctgagata cagagattag gtgctaagaa agtataccta ataggtggag aaaattctat
    10081 aagtaaagaa attgaacaac aattaaaaag cttaaatata tcgatagaga gaatatcagg
    10141 aagtgataga tacaagacaa gtttattatt agctcaaaag ttaaatggta taaaaaatgt
    10201 atctcaaata gcagttgtaa atggagtgaa aggattagct gatgcaatta gtgtaggcgc
    10261 tgcagcagca gaaaataaca taccaatcat acttgcaaat gaaaagagcg aattgcaagg
    10321 agctgatgaa tttctaaatt cattaaatat agagaaatct tatattattg gaggaacagc
    10381 atccctatca aacaatctag aaagtaagtt aaaaaatcca actagattat ctggtagtag
    10441 tagagacgaa actaattcaa agataataga taatttttat aaaaaagata ctttaaaaaa
    10501 tgcatttgta gttaaaaatg gaatcaagaa tcaaaatgat ttaatagatg gattggcagt
    10561 tggtccttta ggagcaaaaa ctgggtcacc agtaatttta gttggtgata aattagctga
    10621 tagccaaaaa gaagttctta aaaataagac tttagaaaaa gtaactcaag ttggtggagg
    10681 agcaaataaa aatgcattta aagagttagt aaaattaaaa agtgctaaat aaggtactta
    10741 agtattaaca tatactagtg tctattagta atttatctta ataaaagctg aaaataatta
    10801 ctttaaatta ttgataaata agataatcaa tgattgtaaa attttatttt cagctttaaa
    10861 t
//
LOCUS       DAKBNN010000135        10794 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000135 DAKBNN010000000
VERSION     DAKBNN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.136"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     regulatory      108..218
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            322..693
                     /locus_tag="OUB02_002752"
     CDS             322..693
                     /locus_tag="OUB02_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HCT8886496.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            788..1735
                     /gene="trxB"
                     /locus_tag="OUB02_002753"
     CDS             788..1735
                     /gene="trxB"
                     /locus_tag="OUB02_002753"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCT8886497.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            1820..2137
                     /locus_tag="OUB02_002754"
     CDS             1820..2137
                     /locus_tag="OUB02_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HCT8886498.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            2406..3692
                     /locus_tag="OUB02_002755"
     CDS             2406..3692
                     /locus_tag="OUB02_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HCT8886499.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            3804..4277
                     /locus_tag="OUB02_002756"
     CDS             3804..4277
                     /locus_tag="OUB02_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3936..3938,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HCT8886500.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            4309..5619
                     /gene="grdB"
                     /locus_tag="OUB02_002757"
     CDS             4309..5619
                     /gene="grdB"
                     /locus_tag="OUB02_002757"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5356..5358,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HCT8886501.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            5876..7408
                     /locus_tag="OUB02_002758"
     CDS             5876..7408
                     /locus_tag="OUB02_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HCT8886502.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            7419..8564
                     /locus_tag="OUB02_002759"
     CDS             7419..8564
                     /locus_tag="OUB02_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HCT8886503.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            8874..9953
                     /locus_tag="OUB02_002760"
     CDS             8874..9953
                     /locus_tag="OUB02_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCT8886504.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            10066..10665
                     /locus_tag="OUB02_002761"
     CDS             10066..10665
                     /locus_tag="OUB02_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886505.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
BASE COUNT         4241 a         1202 c         2152 g         3199 t
ORIGIN      
        1 atagaatgtt tatatactta agtcaacaga gatagaaaaa agctatttat agaaaacaaa
       61 ggataaattg cttaatttta aattttctga aaaaaataat aatccggatg aagagtatgg
      121 gagactgctt tataaaaagc gaccgaaggg acaagtttaa tcttacttac ccaaaagatt
      181 agatgaaatt ctcaggtaaa ggtgactgta cttggacggg gctctgaaaa tgattttaac
      241 agggaagaca aattgggaga tttgtttttc ctgtttttta tttatcaagt aataaataaa
      301 agtttgaaag agagtaaaaa aatgataata attacgaata atccaaaagt aaaagaagaa
      361 gttcaaggca gagaagtttt gtttaaagac acaacttata ttggaatctt agaagcaagt
      421 agagatttga tacatgaagg ttatgaactt ttatctcatc cactctatgg gagtgtaaag
      481 ccaaatgaaa caccttacag gactgttata ctaaagaaag gaaatcgttt ggatataaac
      541 tctttaacct taattgaaga agcaatcatc actgcaagta aatttcagaa taataaaaag
      601 actccaaaat ggacagaaag tgttcaagat gattttagag taattgatta tgatatattt
      661 tataatacaa tacaaagaat gcagtatgaa taaaaaaatt gttaaaaagt tattaaaaca
      721 gcctaaatat taaatctaat ttaatattaa ataaaatgag aaatttaatt ggggaggata
      781 agtcaaaatg gaaaatgtgt atgacttagt aataattggt tcaggaccag cagggcttgc
      841 agcaggtctt tatggagcaa gagcaaagtt aaaaactcta atcctagaaa aagacaagac
      901 tggtggacaa attgtaataa cacatgaaat tgcaaattat ccagggtcag ttccaaatgc
      961 aacaggacca agtttaatag ctagaatggt tgaacagtgt aaagagtttg gagcagaaat
     1021 gctaagagac aacattgttg atactgagct agatggagat ataaaagttt taaaagggga
     1081 aaaggcagaa tatagagcaa aagcagtaat aataggaaca ggagcaacac caagaaagat
     1141 tggttgtcct ggagaaaaag aacttactgg taaaggggtt tcatattgtg caacttgtga
     1201 tgcagatttc tttgaagatt ttgaagtatt tgttgtaggt ggaggagaca gtgcattaga
     1261 agaagcaatg tatttaacta aatttgctag aaaagtaaca attgtacata gaagacaagg
     1321 atttagatgt gctaagagtg ttgaagaaaa agcaaaagct aatccaaaga tagagttttt
     1381 attagataca gtaattgaag aaatcaaagg tgatggaata ttagaatcag ttgtatttaa
     1441 aaataaagtt actggagaaa ctcatgaata ctttgcagat gaagaagatg gaacaatggg
     1501 agtatttgtg ttcgttggat tagatgctca aactgattta ttcaaaggta aagttgatat
     1561 ggatgaaaaa ggttacataa taactgatga agatatgaga actaacatac caggcgtatt
     1621 tgcagcagga gattgtagat ctaagactct aagacaggtt gtaactgcaa ctaatgatgg
     1681 agcaattgca tctatagtag ctgagaaata cattgatgaa aaatttggga actagaatct
     1741 gtaaattaag aaatagtgtc tgtaaattta atgaataata tttaaaatta aaataaaaat
     1801 ttcaaggggg aataagaaaa tgttagattt agataaggct acatttgaag aagaagtttt
     1861 aaatgcagaa ggttttgtat ttgttgattt ttggagtgaa ggttgtgagc cttgtaaagc
     1921 tttaatgcca gatgttcata aattagctga gacttatggg gataaaataa aattctgtaa
     1981 gatggataca acaaaagcta gaagattagc tataaaacaa aaagtattag gtcttccaac
     2041 tatggctata tacaaagatg gagaaaaagt agacgaagtt actaaggatg atgctacagt
     2101 tcctaatata gaaaacatga ttaaaaaata tctttaatat gttgataaat tattaacatt
     2161 aaaagaaaaa atctattttt atctgaagat aagaagaaaa aggtttttat gtccaacttt
     2221 gttaaaaaaa taattttaaa taaaataatt tttttacaat aaatattttt tagtaataaa
     2281 ataaagattt aaggcataaa aacacaataa tatatcttta aaaaaataag ataaattgta
     2341 aaatgatata attttgagaa aaactcaaat gcatatacac aaattaaata acaggaggtg
     2401 caagtatgcg tcttgaatta gggaaaatct ttataaaaga tattcaattt ggtgatgtaa
     2461 cagaagtaaa agatggagta ttattcatca ataagcaaga gatgctacaa gaaattggtg
     2521 gagatgagca tattaaatct atagatatag aattagctcg tccaggtgaa agcgtaagaa
     2581 taacacctgt aaaagatgtt attgagccaa gagtaaaagt tgaaggtaat gggggaatct
     2641 tccctggtat catgtctaaa gttgacactg taggagaagg aaaaactcat gctttaaaag
     2701 gtgtagctgt tgtaactact ggaaaaatag taggtttcca agaaggtata atagacatga
     2761 ctggagaagg agctaagtac actccatttt ctaaattaaa taatgtagta gtaatagctg
     2821 aacctattga tggtttaaaa caatacgctc atgaaaaagc tgttagaatg ataggattca
     2881 aagcagcaat gtacttagga gaagtagcaa gaaatcttac tccagatgaa gtttcagtat
     2941 atgaaactaa acctcttctt gaatcaataa aagaatatcc agagcttcca aaagtaggat
     3001 atgtttacat gcttcagact caaggtttat tacatgatac ttatgtatat ggagtagatg
     3061 caaaacaaat agtaccaact ttattatatc caacagaatt aatggacgga gcaattgtaa
     3121 gtggtaactg tgtttctgca tgtgacaaaa acccaagtta tgttcatata aacaatgggg
     3181 ttgttgaaga cttatatgca agacatggta aagatataaa ctttgtaggt gtaattataa
     3241 ctaatgaaaa tgtttatcta gcagataaag aaagatcttc aaactggact tctaaattat
     3301 gtaaatattt aggattagat gcagttatag tttcacaaga aggttttgga aatcctgata
     3361 ctgaccttat aatgaactgt aagaagatag aaatgcaagg tgttaaaaca gttatagtta
     3421 cagatgaata tgctggacgt gatggaggtt ctcaatcact tgcagatgca gatgtaagag
     3481 ctgatgctgt tgtaacaggt ggaaatgcca accaagttgt agttcttcct aagttagata
     3541 aagttattgg acatttagaa gtagtagatg taatagctgg tggttctgat ggaagtttaa
     3601 gagctgatgg aactattgaa gttgaaatcc aagctataac aggtgcaact aatgaaacag
     3661 gatttggtca tttaactgct aaaggatatt aagaagtatt ttattaaacg attgagtaat
     3721 gttaataaaa catattatag ataattataa taagtgttat agatatataa aaatatatta
     3781 gaaatataat aaggaggatt attatgagtt tacttagtaa taaaaaggtt cttataatag
     3841 gtgaccgtga tggtatacca ggacctgcga tagaagaatg tgtaaaaaca gtagaaggag
     3901 cagaggttgt tttctcatct acagaatgct ttgtctgaac agctgctggg gctatggact
     3961 tagaaaatca aaacagagtt aaagatgctg ctgataaatt cggagctgaa aatgttgtga
     4021 ttttactagg tgctgctgaa gccgaagctg caggtcttgc agccgaaaca gtaactgctg
     4081 gagatccaac tttcgctgga ccacttgctg gagttgcctt aggattaagt gtttaccacg
     4141 ttgttgagga accaataaaa tcattatttg atgaaagtgt atatgaggac caaataagta
     4201 tgatggaaat ggttttagaa gttgaagaaa tagaagaaga aatgtctggt ataagagaag
     4261 aattttgtaa attttaatgc ctaaaagcaa aaggggtgaa actaagagat gggaaaacta
     4321 aaagcagtac attatattaa tcagttcttt gctggtatag gtggagaaga aaaagctgat
     4381 actaagccac atgtggcaga aactttacct ccaataagtt tacaacttga taaattatta
     4441 ggtgaagata ttgaaatagt tggaacagta gtttgtggag atagctattt caatgaaaat
     4501 atagatagtg caagtgaaga agttttatca atggtaaaag gttttgaacc acaacttttc
     4561 atagctggtc ctgcatttaa tgctggtaga tatggggttg ctgctggaac aataactaaa
     4621 gttgtaaaag atgctttgaa tataccagct ttaacaggta tgtatgttga aaatccagga
     4681 gcagatatgt tcaaaaagga tgtttatgta gtagaaactt ctgattctgc agcaggtatg
     4741 agaaaggcac ttcctaagat agctaaactt gcagtaaaac ttgctaatgg agaagaaatt
     4801 ggaactccta aagatgaagg atacatagct agaggaataa gagttaacta cttccatgaa
     4861 gacagaggtt ctaagagagc tgttgacatg ttagttaaga aaataaaagg tgaaccattt
     4921 gaaactgaat acccaatgcc aaactttgat agagttgacc caagtaaagc tgtaaaagat
     4981 ttatcaaaat gtaaaatagc tttagttact tctggtggta tagttccaaa aggaaatcca
     5041 gatagaatag agtcttcttc agcttctaaa tatggaactt attctatagc aggagttatg
     5101 gatttaacag aagagactta tgaaactgca catggtggat atgacccagt gtatgcaaat
     5161 ttagatgcag atagagtatt acctgttgat gttcttagag atttagaaaa agaaggtgta
     5221 attggaaaat tacatgagac tttctataca actgtaggta atggtacatc tgttgcaaac
     5281 tctaaaaaat atgcttctga aattggagca gcattagtgg ctgatggtgt agatgcagta
     5341 atattaactt ctacatgagg tacttgtact cgttgcggtg caacgatggt aaaagaaata
     5401 gaaaaaactg gacttccagt agttcatatg tgtacagtag tacctatttc attaacagta
     5461 ggggctaata gaatagttcc aactatagca atacctcatc cacttggaaa tccagcgctt
     5521 gaccctacag aagaaaaagc cctaagaaga ggccttgtag aaaaagcatt aaatgcttta
     5581 actacagaag tagatggtca aacagtattt gaaaaataaa aataacacac acatatatta
     5641 tatagataaa aatataaaca cacactaaaa ataaaaaata actatacaca aaaaaaataa
     5701 tatagatttc atgaatggat aaattggagt tattcattca tgaaatctta aaaaatgcca
     5761 aaatacataa acaataatag aatcaaatat acaataaaat acaatgatta gagcttttaa
     5821 taagtttaat aagattaaaa aataagtgtt cataaaattt atggaggtgc acaaaatgac
     5881 ttatccagtt ttaaaagggg caggctatgt acttattcat actccagata tgatagtgca
     5941 aaatggaagt acttgtacag ttgaaagagc aacaaatcca gattcagaat ttttaaaaga
     6001 agtaagtaat catataagaa gttatgaaga tgtagttaac tacatgccaa atcaagttta
     6061 tataggaaat agaagaccag aagaattaag agatttacca atgccatggt gtgaacaaaa
     6121 gattgaggga actagaaatg gtaagtttgg agaaataatg cctcaagatg agttcatagc
     6181 tttaatgcaa ataagtgatg catttgattt agtaaaatta tcacaagaat ttattgatga
     6241 agtaaagcca aaaatcgaaa ataactaccc tgaaattgca ccatttgtag gtaaattaaa
     6301 gggtgatgat atagaagaag gtaaagaatt agtagcaaca cacatagctg aaggattata
     6361 ccatgatgga aaatttgttg gttatgtaaa aagagcacat gatgtagatg ttaacttaaa
     6421 tgcccataca atgtttgaaa atctagtagt aaaagcatct ggagttttat ctgctattca
     6481 aatgctaaga catagcaaaa tagaccctgc tgaaatagat tatgttattg agtgttcaga
     6541 agaagcttgt ggagatataa accaaagagg tggaggaaac tttgctaaat caatagctga
     6601 gatagcagga cttcaaaatg caactggttc tgacacaaga ggattttgtg cagcaccaac
     6661 tcatgcatta attcaagcag cagctttagt aaaagctggt atacataaaa atgtaatggt
     6721 agtagcaggt ggagcaagtg ctaaacttgg aatgaatgct aaagaccatg ttaaaaaagg
     6781 tcttcctgta cttgaagatg ttgttggagg atttgcagta cttgtatctg aaaatgatgg
     6841 tgtaaatcca gttataagaa ctgatttaac aggtaagcat actgttggaa ctggttcttc
     6901 accacaagca gttatgacag cacttataac ttcaggtctt gatagagcta atttaaaaat
     6961 aacagatgtt gatgtgtatt cagttgaaat gcaaaaccca gatataacta agccagcagg
     7021 agcaggagat gttccagaag caaactacaa aatgataggg gcacttgctg ttaaacgtgg
     7081 agacttagaa aagaaagaat tgaaagattt tgtttctaac aaaggattac caggatgggc
     7141 accaactcaa ggacatatac catcaggagc tccttatata ggatttttaa tagatgattt
     7201 aactacagga aatagaaata gagctatgat agttggaaaa ggtagtttat tcttaggaag
     7261 aatgactaac ttatttgatg gagtatcttt tatagctgaa agaaatacag gtgttactga
     7321 agagacttca ggaatatcta aggatgaaat taagaagata atagctgaat ctatgaaaaa
     7381 actagcatta gatatgttag aagaataggg gtgaggatat gtcaaaaaaa gtaatcgcag
     7441 atgtattctt agaagtagct aatgcaatag aaagcggcga atttggcaaa aaagtaaaaa
     7501 ttggtgttac tacacttgga agtgaacatg gtgtagaaaa catggttaat ggtgcacaat
     7561 tagcaaaatc taatttattt gatattgtgc ttattggacc aaaagtggaa actgaccttg
     7621 aagtagttga agttaatgat gaaaaagaaa tgcatgcaaa aatggaagaa ttattagatt
     7681 caggatatat tgatgcttgt gttactatgc attataattt tccaataggt gtatctacag
     7741 taggtagagt aataactcca gcaaaaggaa aagaaatgat acttgcaaca acaacaggaa
     7801 caagtgctac taatagaata gaggcaatgg taagaaatgc catttatggt attgctacag
     7861 ctaaatcaat gggtaacaaa tgcccaaaag ttggaatact taatgttgat ggagcaagac
     7921 aagttgagaa gtgcttaaaa gaattaaaag ataatggata tgacatggag tttgcggatt
     7981 caattagagc agatggtgga tgtgtaatga gaggtaatga tttacttgta ggtgcacctg
     8041 atgttatggt tactgataca ttatctggaa atatatttat gaaagtattt tcttcatata
     8101 ctacaggtgg agattatgaa gctcaaggct ttggatacgg acctggagtt ggagaagact
     8161 atgatagaaa ggtacttata gtatcaagag catcaggttc tccggtagta gcaaatgctt
     8221 taaaatacgc ttatgatgtt gtaaagggag atataagtaa tgttgcaaga aatgaatttg
     8281 caaaggttaa aaaagcaaaa tttgatgaca ttatatcctc tcttactaaa aaagaagtta
     8341 aagctgaaaa agtagaagtt aaaatgccag ataaggaaat tgttactcgt caaatagctg
     8401 gtgttgatat aatggattta gaagatgcag tatctgagtt atggaaaaac ggaatctatg
     8461 ctgaaagtgg tatgggttgt acaggtccaa tagttttagt aaatgaagct aaaggagact
     8521 tagcagttga aactttagtt aaagctggat atactgcaaa ataagtacat tttttatatg
     8581 taaataagaa catatagtta taattttatt gattataatt atatgttctt tttattttat
     8641 atcttgtcaa tatttatata ttaacacaat ccatttattt gattctctag ctaatttatt
     8701 atatttattc aattaaaatt aagctttcta actaaataaa aactaatgaa gtattaaaat
     8761 gaaattgata aaatatgaaa aaattttagg aataaagtat ctgttagata aaaataagat
     8821 taattagtga tataatataa ttaagataaa atttattatt tggaggtaaa atcatgagca
     8881 gagtaaaaaa tgtagtagaa ttgttggaaa ctaaaggtgt tgatgcacta tatctaacaa
     8941 agaaaactaa tgtaaactat ataagtggtt ttcctgatga agaagcatat gcagtgattt
     9001 gcaaagatgg aaacttttta gttactgata gtagatatat ggagcttgca gaaaaggttt
     9061 gcaaagattt tgaaattata aattggcata attttgatag aagtgtggca aaggcagtta
     9121 aaagtgtatg tgataaagtg ggtataaaga aacttgggtt tgaaagaact aatatagtgt
     9181 ttgataaata tgaagaactt aaaaatctga ttgagaagga taatggagaa ttaataccta
     9241 cagaaaatat tgtagagact ttgagatatg taaaagacaa agatgaaatc aaaaatacaa
     9301 gaaaagcttg tgaaatagcg gataaggcat tagaagaatt gattccacat ataaaagctg
     9361 gagttagcga aattgaactg gctacaaagt tagagtactt tatgaaaatg aacggagcac
     9421 aaaatatagg gtttgaaact atactgatat caggagcaaa gacttcattg cttcatggta
     9481 agcctagtga taagattata gaaaaaggtg attttgtact tatagactat ggtgctatgt
     9541 ataatggcta tatatctgat acaactagaa cttttatagt tggaggagct tctgaaaagc
     9601 aattagaaat atataattta gttaaggaag ctcaaaatgt aggggttgag aacatgaaag
     9661 ctggtgtaca tgcgacaatt ccagatgctg agataagaaa agttgtcaaa aaatatgaag
     9721 actattacta tcaaggtata ggacatggag tcggtagaga tgttcatgaa gaacctttca
     9781 ttggtaatta tggtgataaa atcatagaag agggatgtat aataacaatg gaaccaggta
     9841 tttatttccc tggatggggt ggagttagaa tagaagatac tgttttgatt actaaaaatg
     9901 gtccagaaag acttactaaa ttccctaaag atttgatgat tttagacaaa taatttaatt
     9961 tatatatatt tttttacata aaatataaca taatctcact attaagctct atttacatat
    10021 tatagtaaag agggcttaat tttattgtga gaggtgatag tatatatgta taaaaaacat
    10081 aaatttataa aaaatataaa aaaagcctgg tataaaatta aattaattga tagatgcctt
    10141 ataattgtaa tggcaatatt aatgtttcaa tctatatata atttatttgt taatgaagta
    10201 aattcacaag atactacaac tattgatgta gtagtaagaa ccacttcagc agctatattt
    10261 gggtattttt taagtgctaa ttttataaag agacctttaa ggaagtatac aaatactgat
    10321 gtatcttcaa atctttttat agataataat ttatccccaa aaggtcaaga gtctaatcaa
    10381 aataatataa tgaatgtcaa aaataccatt ggatttactt cagaagctga taattatgaa
    10441 aagaaaatac ctattaataa taatgaaggt tttgaagaag gagaaactag tgaattgcaa
    10501 attattatag ctactgtaat ttgtatagta gcattaacag tactttttgt tgtaagaaat
    10561 tttacaacta caacatcagc ttctttaggt actatttcac agatgagaga ttttgtctca
    10621 ggatgcgtgg gttttttgct aggttgtcca agtaaacctg attaaaaaag tattatcaat
    10681 aaaaggtatt ttaaaaagta tcagtataat aatatttaga tttaaatgta tacaattata
    10741 attctataac attctatatt gtatttttat actgatactc taaactatac tatt
//
LOCUS       DAKBNN010000136        10768 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000136 DAKBNN010000000
VERSION     DAKBNN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.137"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            308..766
                     /locus_tag="OUB02_002762"
     CDS             308..766
                     /locus_tag="OUB02_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8886506.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFEEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            827..1102
                     /locus_tag="OUB02_002763"
     CDS             827..1102
                     /locus_tag="OUB02_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8886507.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1161..2435
                     /locus_tag="OUB02_002764"
     CDS             1161..2435
                     /locus_tag="OUB02_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCT8886508.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2565..3617
                     /locus_tag="OUB02_002765"
     CDS             2565..3617
                     /locus_tag="OUB02_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT8886509.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DNHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3617..4648
                     /locus_tag="OUB02_002766"
     CDS             3617..4648
                     /locus_tag="OUB02_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCT8886510.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     KESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4740..5552
                     /locus_tag="OUB02_002767"
     CDS             4740..5552
                     /locus_tag="OUB02_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCT8886511.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5555..6475
                     /locus_tag="OUB02_002768"
     CDS             5555..6475
                     /locus_tag="OUB02_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HCT8886512.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6650..7096
                     /gene="rpiB"
                     /locus_tag="OUB02_002769"
     CDS             6650..7096
                     /gene="rpiB"
                     /locus_tag="OUB02_002769"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HCT8886513.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7126..7791
                     /locus_tag="OUB02_002770"
     CDS             7126..7791
                     /locus_tag="OUB02_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCT8886514.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7901..9403
                     /locus_tag="OUB02_002771"
     CDS             7901..9403
                     /locus_tag="OUB02_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCT8886515.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFAGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9715..>10768)
                     /locus_tag="OUB02_002772"
     CDS             complement(9715..>10768)
                     /locus_tag="OUB02_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8886516.1"
                     /translation="SNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYA
                     EAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEI
                     STLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDF
                     DRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFE
                     DKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEE
                     NIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEII
                     IDADLFKVMLTFRRVN"
BASE COUNT         4300 a         1214 c         1973 g         3281 t
ORIGIN      
        1 ttttataaaa tagttgttaa acataaaaaa ccatttgtaa aaaacaaaaa aatcatttct
       61 ctaacaaaaa aaaccacaaa aaatataaaa aagtatggct agttaagtga ttaacaagta
      121 aaaattaaaa taaaaatcaa aaaaaattta tcaatgaaag tcagctaaaa tcctggaaga
      181 gagcattatt tttgggaaaa cattttcttt tgtttcacaa aaaaagccac tttttggcac
      241 acaacatgct ttatataaag tcaagaaaaa ctaaggtata agacaaaggc aattaggagg
      301 tgtaaatatg gagtttataa attttgtaag tgataaagtt atagacctta aaatggattg
      361 taaaacgaaa gaagatttct ttgaagaaat acataataaa gtttttgatt taggctttgt
      421 aaaagaagaa tttggagaaa aaatattagc aagagaaaat gtatttccaa caggtttaaa
      481 tttaggagat tatggagtag caataccaca tacagatgca gaatatatta aagagcaatt
      541 tatatcagtc tgtactttta atgaaccagt agtgtttagt tctatggaag accaagatga
      601 aaaagtacct gtaaatttgg ctttcgtatt aggacttaat caaccacaca gtcaattatc
      661 tgtattaaca gaattaatgg gaattatgca aaataaagag ttagttgata agttgatgag
      721 ttctactgat aaagaagaag ttttaaagac actaaagtct ttataaaaat aaaaaagtac
      781 taaaaataaa aaatataaaa ataaaaatta ttaggaggaa ataaaaatga aaaaaatatt
      841 agtagcatgt ggagcaggaa tagcaacatc aacagtagtt tgtgacaagg tagagagatt
      901 agttaaggaa aataatatac aagcagaagt tatacaatgc aaaatagctg aatgtgcaac
      961 aaagcaagaa ggagcagatt taatagtatc aacaactata ctaccaacaa cttataatat
     1021 accagcaata aaagcaacag catatataac tggagtaaat acagcagcat tagataaaaa
     1081 aatattagat gctttaaaat aatcctatca tagatacatt gaaataatat tttataaaac
     1141 agaaaaagga ggatttataa atggataaat tattagcagt agtacaatac attttaaata
     1201 tgggaccgac agtaatacta ccaataacaa ttgtagtact aggaatgata tttagaatta
     1261 agtttacaaa agctttaaag tcaggtctta caattggtat aggatttgtg ggtataaact
     1321 tagttgtaaa cctattaaca tcaacactag ggccagcggc tcaaggaatg gttgaaagat
     1381 tcggacttaa tttaacagta atagatgttg gttggccaac aacagcatca gcaacatggg
     1441 cttcaccagt tgcaccaata ttaataccag tttgtttatt agtaaactta ctattattat
     1501 acttcaataa aacaaagata ttaaacgttg atatatggaa ttattggcac tttatagtag
     1561 caggtgcagt tggatatata gtaacaggaa gttatgtttg ggctatattc tgtgcaatac
     1621 taatggagtt attagtactg tttattgcag aaaagacagc aaaaaatgtt caggagtttt
     1681 atgatttaga aggtatagca ttaccaactg gttcaacagc ttcatttgct ccacttggat
     1741 atttagtagg taaatttgtt gaaaaaatac caggaataaa taaaatacaa gcagacccag
     1801 actctataca aaaaagattt ggtatatttg gagaaccaat gatgatgggg attatactag
     1861 gtataatact tggagcactt gcgggatatg atgtagcagg aatattcaat gtaggtatat
     1921 caatgggtgc agtaatgtta cttatgccta gaatggttag aatattaatg gaaggtttaa
     1981 ttccaatttc agaatcaata agagatttct tacaagcaaa atatggagac agagaacttt
     2041 atataggtct ggatgcagca gtagcaactg gacatccagc agttatatca acagctctta
     2101 tacttgttcc tataactttg ttcttagcag ttatactacc aggaaacaaa gttttaccat
     2161 ttggagactt agcgacaata ccattttata tggcatttat agtgtgtttt agaaaaggta
     2221 atatagttca atcagttata acaggaacaa tagtaatagc tatatcactt tatatggcta
     2281 caaactttgc agatgttcat acacaattat tacaacaagc aaatatgatg ccagaaggaa
     2341 caacaatggt tacaagtata gatacaggtg gaaacttgct aaaatgggga atattgaaat
     2401 tatctcaatt agtttcttca tttggaatat tctagtatgt agttaataga gattactctt
     2461 ttttagtaaa gtatgcgaaa atttaacatt tagcagctac taaaaaagag taatattttt
     2521 tataaatttt taaaaataca aaatacaaga ggaggagatt tatcgtgaaa gcagcagtat
     2581 tacatggcac aaatgatatg agatttgaag atatagaaat aaaaccatgt gaaagtgatg
     2641 aagtaaaaat aaaagttatg gcagcaggaa tatgtgggtc agatcctcca agagtattaa
     2701 aacattggaa atatccagtt ccagctatac caggacatga gttttctgga gtaattgctg
     2761 aagttggtaa ggatgtaaaa aatgtaaaag ttggagatag agtagttgca attcctttta
     2821 taccttgtaa tgaatgtgaa tattgtaaaa gaggattatt ttcattgtgt gataaccatg
     2881 gaatgttagg agctaaaagt tttggagctt ttgcagagta tgtaaatata aaagctacaa
     2941 atgtacttcc aataggagat atgaactttg aagatgctgc aatgatagag ccactagcag
     3001 ttgctatgca tggagtactt aatataggtg ttcaagttgg agacactgta gctgtaatgg
     3061 gtagtggaac aatggggcag ttggtaatac aaggacttaa aatagcaggg gcaggaacaa
     3121 ttatagcagt tgatatatct gataataaat taagagaatc aaaggaatta ggggctgata
     3181 taataattaa tgctaaagat attaacccag tagaaaaaat aaaagaactt actggtggaa
     3241 aaggtgttga catagctctt gaatgtgcag gttcaaaaat aactcaagaa caatgtttac
     3301 ttataactaa aaagaaaagt aagataggat ttttagggat agcatattca gatataactt
     3361 tatcagaaga agcatttgaa aatatattta gaaaagaact agaattaaaa ggtttttgga
     3421 actcttattc tgcaccattt ccaggacaag aatggactaa gggaatcaat ctagttaatg
     3481 aaggaaagat aaaattaaaa gaaatggtat ctcatagatt ctcacttgaa gatacttaca
     3541 aagcatttga aatgataaga gatagaaaag aagaatttaa caaaatatta attttaccac
     3601 aaggggttga aaaataatga aagcagtagt taaaactaaa ccaggctatg ataatgtaga
     3661 agttttagaa gtagaagaac caaaagctac aggagacaag gttaaaataa aagtagaata
     3721 tagtggtata tgtggttcag atatacattc atttaaaggt gaatatgcaa atataaaagc
     3781 cccagtaaca ttaggtcatg aattttctgg aattgttgta gaagtaggag aagatgttaa
     3841 aaatataaaa gtaggagata gagttacatc agagactact tttgcaactt gtgagaaatg
     3901 tatatattgt gaaactaaag attataactt atgcccaact agaaaaggta ttggtactca
     3961 agtaaatgga agttttgctg agtatgtatt atcaagagaa gaaagcatac atgtacttcc
     4021 tgaaaatgta tctttattat cagcagccct tacagagcca ctagcttgtt gtgttcatgc
     4081 agctcttgaa aagactacta tagaaagttc tgatacagtt ttaataatag gaccaggacc
     4141 aattggttta ttattagctc aagttgttaa atctcaaggt gctacagtta taatgtctgg
     4201 tgttacaaaa gataaggaaa gattagaaat agctaaaaat cttggagtag atagaactgt
     4261 aaatgtaatg aaagaaagtt tggcagatgt agtgaatgaa atgactgatg gaaatggagt
     4321 aaacaaagga tttgattgtt ctgggtttat gccagcagtt aacgaagcct taagactgct
     4381 tagaaaaaaa ggtactttcg tacaagttgg aatctttgct aaaaaattaa atgaaatgga
     4441 ccaagaagca ataatccaaa gagagttaca atatatagga agcaggtctc aaaagccaag
     4501 ttcttggatt acagcattag atttaatggc aaatgggaaa gtaaatacag aagctttagt
     4561 tactagaact gttggtttag acgaatttaa agaaggtatt acagctttaa tgaacgggga
     4621 agaaataaaa gtagcagtaa aatcttaaaa aaacatttgt attattttaa aaaaaattgt
     4681 aaaatctact gtaggataaa aaaatgtaag gtcttaaaaa ataacaggag gaagaaagta
     4741 tgagcactct tagagatcat tctaataata ttagaaaaaa cataataaaa atggttgcag
     4801 aagctaaaag cggacatcca ggtggttcat tatcaatagc tgatatgttg acagtattat
     4861 attttgataa gatgaacgta tgtgcagaaa atccaaaaat ggctgataga gatagatttg
     4921 ttctatcaaa aggtcatgca gcaccagctt tatatgctac attagcagaa aaaggatttt
     4981 tcccagaaga agaattaata acactaagaa aatttggttc taagttacaa ggtcatccag
     5041 acatgaaaaa agtagcagga atcgatatgt ctactggttc tcttggtcaa gggctatctg
     5101 cagcaaatgg tatggcactt gcaggaaaat tagataacaa agactataca gtatatacta
     5161 tattaggtga tggagaaatc caagaaggtc aaatatggga agctgcaatg acagcatcac
     5221 attacaaatt agataattta attgcatttg ttgacttaaa tggattacaa atagatggtt
     5281 ctaatgaaga agtaatgaat gtaaatccag tagatgacaa attcaaatct tttggatgga
     5341 atgtaatagt aataaatgga cattcttttg aagaaatagg aaatgcagtt gatgaagcta
     5401 aaaaagttac aggaaaacca actgtaataa tagctaaaac agtaaaaggt aaaggtgttt
     5461 catttatgga aaataatgct gcatggcatg gaacagctcc aaatgcagaa caagcagagc
     5521 aagcattgaa agatttagaa ggaggtctat aggaatggga atagcaacta gagaagctta
     5581 cggacaagtg ttaaaagaac ttgcagaaaa taaagatata gtagtattag atgcagattt
     5641 aggaaaagct acaaaaagta tatcatttaa agaagttgcc ccagatagat tttttgacat
     5701 ggggatagca gaaggtgaca tgattggtac agcagcaggt cttgctactt gtggaaaaat
     5761 accttttgcg agtacttttg caatatttgc agcaggaaga ggctatgagc aaataagaaa
     5821 ctctgtagca tatccaaatt taaatgtaaa aatagcagct actcatgcag gtgtaacagt
     5881 aggtgaagat ggtggtagcc atcaagctat agaggatata tctctaatga gaggtatacc
     5941 aaatatggtt gtattaaatc cagcagatgc tttagaagca agacaagcta ttcttgcatc
     6001 tattgattat aatggacctg tttatataag attaggtaga gctgcaactc ctgatgtaaa
     6061 cagtgaaaat tataaatttg aaataggaaa aggaactgtt ttaagagaag gttctgacat
     6121 aacagtaata gctacaggaa taatggtagc aaaagcctta gaagcagcag aagaacttgc
     6181 taaagaaggt ataaatgtag aagttgttaa tatatctaca ataaaaccat tagatgaagc
     6241 attaataaaa gaaactgcta agaaaactgg aaaagtagtt acagcagagg agcacagtat
     6301 aataggtggt ttaggttcag ctgtatgtga agctttagca gaaactaaag atgtagtagt
     6361 tagaagaatt ggtgttaaag atgtatttgg tcaatcaggt acaccagctg atttattaaa
     6421 acactatggt ttaactacag aagatatagt aaaaaatata aaagagttat tatagatata
     6481 aatatatcta aataacaaat tgatttgaca taagtcaatt aaagctgtct taaaaataga
     6541 aagaaatttc tacaatggga cagctttatt tttacaatac tttattttta tgataattat
     6601 aaaatagtct gaaagtaatc aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc
     6661 tattggatgt gaccatggtg gttatgaatt aaaggaatat ataaaggaaa atttaataaa
     6721 taaaggtata gaagttgaag atgtgggtac aaattcaaaa gaaagtgtag attttccaat
     6781 atatgcaaaa aaggtaagtc aaaatgttca aagtaaaaaa tctgatttag gcatactttg
     6841 ttgtggaaca ggaataggta tgtctattgc agcaaataag tttaaaggaa tcagagcagc
     6901 tgttgtttct gactgttttt cagctaaggc aactagagaa cacaataata caaatgtact
     6961 ttgtttagga gaaagagtta ttgggaaagg attagcaatg agaattgttg aagaatggtt
     7021 aaattcaagt tataatggag aaagacatgc aagaagatta caaatgattg atgatataga
     7081 aaaagaaaat ttataaatat ataatttata acaaggggga gcgttatgtc tattatttgt
     7141 gcttcaataa tgtgtgcaga ccaaatgaag ttaaaagagg aattagaagc tcttgaagaa
     7201 gcaggtgtaa aattacttca ttgtgatgtt atggatggaa tatttgttaa aaacttggct
     7261 atgggaccag agttattaaa atcaataagt gaaaatacaa caataccttt agacatacac
     7321 ttagccacag aaactccaga taaatatatt gatatgatgt cttatataaa gccaaaatat
     7381 atttcttttc atgtagaatc aagtacaaat gtaaaagctg atatacaaaa gttaagaaat
     7441 tatggtatag ggccagtgtt agcaataagc cctcaaacaa gtgtggataa aatagaagaa
     7501 tatataagtt tagttgaggg tatcttagta atgacagtaa atccagggtt tgcaggtcaa
     7561 aagtttaatc tttctgtatt ggataagttg gataaactta cagagatact aaaagattat
     7621 gataatcctc catttataga ggttgatgga aatataaata aggatactat taaacttatg
     7681 aatggtaaaa aagtagatat atatgttgtt ggtacttcag ctttatttaa tgataagcct
     7741 ccaatttcat ataaagataa aatagaagaa ctaaaagaaa gtataaagta accatgtaat
     7801 aaaattataa tatgtttctg tttaatagta aaaatttagg tggaaacaag aaattttata
     7861 atatttaata aaaattttgg ttgagtaagg agaatttttt atgggagacc tatataaact
     7921 acaaaatggt actgatatta gaggaatagc gtatgaaaat gattctaaag aagttaacct
     7981 aactgtagaa gaagtaaaaa aaatagctaa agcgtttcat atttggttaa aagaaaagac
     8041 taagaaagat aaagtaactg ttgccatagg aactgattct aggataacag gaagccaatt
     8101 tagaagtact gttattgaaa ctttaactaa tgatgattgt aatgtaattg attgtgagat
     8161 tgccacaact ccagctatgt ttatgacaac tattatggat ggatacaatt ctgatggggc
     8221 gattatgatt acagcaagtc acttacctta ttactataat ggtttaaagt ttttcacaga
     8281 aagtggaggt ttagaaaaaa ctgatataaa agagatgttg gatatagctg ttaaaaatga
     8341 tagtgtagac tatgaggaag taaataaaaa aggtgaagtt atagtaaaaa atttaataga
     8401 agattattct aatttattga tagataaaat tagaaaaggt gtaaattcaa gtaaaaatta
     8461 tgaaaagcct tttgctgggc ttaagatatt ggttgatgca ggaaatgggg ctggtggatt
     8521 ctttgctgaa aaagttttac acatactggg agcagatacg acaggtagtc agtttttgaa
     8581 cccagatggt atgttcccaa atcatatacc taatcccgaa aacaaagaag caatggaaag
     8641 catatgtaaa gctgtgttgg ataataagag cgacctagga ataatctttg atacagatgt
     8701 agacagagct gctattgtag gtaaaaatgg caaaccaata aataaaaatg ctcttatagc
     8761 tgttatatca tcaatagtat tagaagaaca tccaaaaaca gctatagtaa cagattcgat
     8821 tacttctgaa ggacttgcta aatttataaa tgaattacaa ggaagacatc atagatttaa
     8881 aagaggatat aaaaatgtaa taaatgaagc tataagatta aatagtgaag gtgaagagtg
     8941 ccatttagct atagaaacgt ctggacatgc tgctttaaaa gaaaattatt tcttagatga
     9001 tggggcatat ttaatagcta aaattttaat aaaagtagca aagctatcct tggaaggaaa
     9061 gactatagaa gaattgatag aaaaccttga agaagctcaa gaagagaaag aagttagaat
     9121 aggcattaat aaagaagatt ttagaacata tgcagaggat atattaaaag atttagattc
     9181 tcatgttaag agtattgatg gatggtctgt agcaccaaat aattttgaag gcataagagt
     9241 taattgtgat gaatcaaatg gagatggatg gtttttactt agaatatcac tacatgaacc
     9301 attattagct ttaaatattg aatcaaataa aattggcgga gcagaacaaa tttattctaa
     9361 gttaaaactt ttcttagaaa aatatgattt aaaaggtctt tagggtaaat agaaagaata
     9421 tatttatatt gaagaataaa tgtaaatttg tacctaagat tacaaatatt acaaaattgt
     9481 aatcttaggt aacagagatg taactgatta tggaaacact atgtgatatt atagatacag
     9541 agagatgaaa tatgtgatga attatagaga aatataattt aaaatgtatt aatatcacac
     9601 caaaaaggta taatcaaaaa atatgtaaat ataaacccca acatttagaa aaagacctct
     9661 aaggaggttt ttttcttgtt taaataactt aaaatatatt tttatattaa tatatcaatt
     9721 aactcttcta aaagtaagca ttaccttaaa taaatcagca tctataatta tttccatgtg
     9781 tgcattttct aattcaatta tattttgagc aattgataaa ccaagaccag agccatctgt
     9841 ttttctagat atatcacccc ttctaaatct ttctattaac tcttctggtt ttagatttag
     9901 tttatcattt gaaatatttt taaacgttaa tattatattt tcttccttta taaacatatc
     9961 tatatagacc ctagtgttag gcatagagta ttttagtata ttgcaaatta gattctcaaa
    10021 aactctccaa gtttttttac catctatcat ggtaaaaaca gcatattcag gtgtttctat
    10081 tataaaatct aaactgctat tttgaatttt gtcttcaaat tctacaatac tttgaattaa
    10141 aagttctctt atatcggttt ttgttatatt aaattctaca tctccactac ttagcttaga
    10201 tgcttcaaat atgtcatcta ttaataattt taatctttgt gattttttat ctaaaatatt
    10261 tatatagtca tttatagtct ctctgtcaaa gtcattattt tttaacatat caacatagct
    10321 taatatagat gtaagtggag tttttaagtc atgagatacg tttgtgataa gctctgcctt
    10381 catcctttca ttttttatat tagcatctac agcagtttca agaccgcctg ttatattatt
    10441 tatacttttg gctaaagtag atatttcatc atctcctata acttctaatt tatactttaa
    10501 attgccattt tctatatttt ttatattttt tgatatgtta tttatgtaat taaacttaac
    10561 agataaaaag ttgtatatat aaaaatgaaa aactgtgttt acaagtgtaa ataaaattac
    10621 tatagctatt gcctcagcat aatatatata cataaagaat atcaaaacaa gatattggat
    10681 aatagcttgt acaactatag atattaaaaa tactatagct aattttttat ttatagtttt
    10741 tttattatac atactaagta agtttgaa
//
LOCUS       DAKBNN010000137        10489 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000137 DAKBNN010000000
VERSION     DAKBNN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.138"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            170..973
                     /locus_tag="OUB02_002773"
     CDS             170..973
                     /locus_tag="OUB02_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT8886517.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILIFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1072..2033)
                     /locus_tag="OUB02_002774"
                     /pseudo
     CDS             complement(1072..2033)
                     /locus_tag="OUB02_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(2348..3298)
                     /gene="hpdA"
                     /locus_tag="OUB02_002775"
     CDS             complement(2348..3298)
                     /gene="hpdA"
                     /locus_tag="OUB02_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HCT8886518.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNVHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3291..3548)
                     /gene="hpdC"
                     /locus_tag="OUB02_002776"
     CDS             complement(3291..3548)
                     /gene="hpdC"
                     /locus_tag="OUB02_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HCT8886519.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3552..6260)
                     /gene="hpdB"
                     /locus_tag="OUB02_002777"
     CDS             complement(3552..6260)
                     /gene="hpdB"
                     /locus_tag="OUB02_002777"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HCT8886520.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEIKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6557..7573)
                     /gene="tsaD"
                     /locus_tag="OUB02_002778"
     CDS             complement(6557..7573)
                     /gene="tsaD"
                     /locus_tag="OUB02_002778"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCT8886521.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7579..8055)
                     /gene="rimI"
                     /locus_tag="OUB02_002779"
     CDS             complement(7579..8055)
                     /gene="rimI"
                     /locus_tag="OUB02_002779"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCT8886522.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8045..8761)
                     /gene="tsaB"
                     /locus_tag="OUB02_002780"
     CDS             complement(8045..8761)
                     /gene="tsaB"
                     /locus_tag="OUB02_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCT8886523.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVDFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8761..9213)
                     /gene="tsaE"
                     /locus_tag="OUB02_002781"
     CDS             complement(8761..9213)
                     /gene="tsaE"
                     /locus_tag="OUB02_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCT8886524.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9352..10392)
                     /locus_tag="OUB02_002782"
     CDS             complement(9352..10392)
                     /locus_tag="OUB02_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HCT8886525.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3375 a         1909 c         1076 g         4129 t
ORIGIN      
        1 aaaattaata tggataaata ttctaaaccc agtatttatt aaaaatgaaa taaaaagaaa
       61 aaaaatacaa attattatct ttttctttat aaactttgat ttaacttatt cattaggttt
      121 actgttatat tataataaat ttattgatcc agaaaacttt gcatcattca tgatgatatt
      181 acctctatct tcagttgcta ttgcaaagtt ttatactgaa ggattaaata atgatatgta
      241 tgaattttat tcactaatca tattattttt tcttatttat tcattattaa ttgttcttaa
      301 gttattaaat ctaataaata ataatgaatt tataattata aacctaatat taatttttat
      361 aagtagctta tcaattataa tatattcatc aaataaaaaa aatttatatc ctgtaaaaaa
      421 tattaaacta ggttctttat taattattta ctttatgatt tctgtaattg ttccaacatc
      481 atttatacta atactacaag gggctcaacc taattatagt ggtatcataa aatttatatt
      541 tactccaata acatcattaa ctactattta tatttttttt agtgaagaat atgcctggag
      601 aggtttttta cagaatattt tttttgataa atttggtaaa aagttaggag ttataatatt
      661 aggtatgtgt tggagcttgt ggcacttacc actaatattt acattatata cacctgaagc
      721 gccaatatta ggaataatat tacgttcaat acatatagtt ggaatttcaa tatttttagg
      781 atatctttat ataaaaacta agaatatctg gttttgtgct ataattcatg ttttaaataa
      841 tagtgctttt cttatcacaa attctaaaga atcaactatc acttaccatg atatactaat
      901 agtatctatg gttgttttaa ttttttatgt tccatttcta tttacaaaag aatataaaaa
      961 taacttggag taaatagaat atccaagtta tttttatatt tttgcaaatt tccaacaatc
     1021 ctacagaata ctttaagctt agaagatatt attaaaattt attataatca actaattctt
     1081 gatattggtc acatttactt tatcactaac aaaatcatac ttagaatctc tatagtaata
     1141 ccatacaaat ataaagctaa gtagattaac aaaagtagtc aaaattccac cttcaatacc
     1201 aaacatccct ccatttaata gattaccatg agtgatttgt gtagttataa tccctggaac
     1261 ctgaagtcca cttacaggca taccatatat aatcccctga aatacattcc atgtaaaatg
     1321 ataacctata cacatccata tattcgacga ctttataaac atatatgaaa aagaatccca
     1381 ataattataa tattaattac agacaaaaaa gtaacatcag gattccatat atgaatcaac
     1441 ccaaaaataa caaatggaac aatcattata aaataaatat ttttacatct tcgtaaaact
     1501 gccataaaaa aaccacgatt taaaatttct tctgcaaaag caactaaaat aaagaccagt
     1561 atccaagaaa ctgttaaagg tgaaattttt ggtacccatg aagtaacatg accaccacca
     1621 attgttattg ttataataaa gattactgta caattaaata ttcctaataa cattcctaca
     1681 tatccttctt ttttcttaat tctataagaa tgtaatccca tttcactaat aggatttttc
     1741 ataataaatc tccatacaat tataggaata ataatcataa gtgaatctgt tagaatttga
     1801 aatacaatct gtaacccaac atcatttaac caattagcat attctgaaaa atagtttgtt
     1861 tttaaattaa tgtcacctgt agcaaccagg atttttctta ttatttccac aagtatcatg
     1921 cttaaataat actgaagtat gtaaaaggta gccataacag ctataataat ccaccctgaa
     1981 cgtacttttc tgtctttatt cttaataatc aagttgctcc atgatttgtt catatagatg
     2041 tcttcctcct aactattatt tttagttgtt tctaaaatat attctattaa attttttcat
     2101 agtagtattt taccacaaaa cttctgcttc aataaagtaa acgtaatata ttctctaact
     2161 gtaatttaaa ctataaaaaa tatataaagt atatttaaag caatatttat ggacataaag
     2221 caatataaac aatatataaa acatataaaa ctttacttaa ttatttctac ataaaaacca
     2281 cataaaaaag gatgtaactc tagcttttag tgtaacatcc ttttttaatt aaaacttgta
     2341 tttaatttta gaaagctgtc tcatgaccaa cataacaagc tataccatta tccaaaaata
     2401 tatcttgtaa ttcttcaaga tgaccttcat caacatcacc cttatcagaa tactcatatt
     2461 ccttgcctaa ttgagtccat tttgattctc ctaatctgtg aaatggtaat agattaattt
     2521 ctaccaaatt atttttgtgc ataaaagata taatatcact tatattttca tcactatcat
     2581 taaacccaga gataactggt actctaagta ctaatcttcc attccaatca gaatttgcaa
     2641 ggtttgatat attttctaat attaaatcat tatacacacc tgtttgttct ttgtgttttt
     2701 ctctatccat atgctttata tcaataaatg caaaatcaat atcgttaaat attttattaa
     2761 aaacttcatt tgatacacat gcactcgttt caatggcagt gtgtacattt acctcatgac
     2821 actttaataa tacttcatgt aaaaattcat gctgaagtag tggctcacct ccactaaatg
     2881 ttactcctcc attacttctc caattgttag aatcacgttt aattacctgc acaagttcat
     2941 ctacagtgta aggttttgca cataatttaa acgcattata ataacatgag ttgacgcatt
     3001 caaaactttc acagtcctta cagatgttcc aatctataac tggcttatta tcaagattaa
     3061 agctcaatgc accattttta cacttaccat gacatacagt acatccattt tcatattgac
     3121 aagataattc actaaacatc atatgtggtc ttacagtcca actttctgga tttgcacacc
     3181 atttacaact cagtggacat ccatttaaaa atacagttgt tctacaacct ggaccatcat
     3241 gaactgaaaa gctttgtaca tcgaaaatca tgccttctaa ttgcttttga ctactcatta
     3301 aacacatgtc cttcacaagt aattgcattc aatgttgaat atacccaatc ttctttctct
     3361 aggccaacac atttgcctat accttcatca ttagcttcaa caaaattttt acaattttta
     3421 cattttggca ttacttttat ctctggacat gctgcatcat ctaaattaat catttgtttt
     3481 gataatctac aaattccttt ggttgcatct actgcacaaa aattcataca atcactatgc
     3541 tttctcatat tttacacccc ttcatactct gttctagcaa ttacttcatc ttgtattggt
     3601 ttacctaatt cacaccaata ttgagtaaac ccagcaacac gtaccattaa ttgacgataa
     3661 ttgtcaggat ttttttgtgc atcttttaag gtttctgaat ctacaacatt atattgaata
     3721 tggaacccac cttttcttaa ataacttctt gttaaatcta ataatttttt agttccttgt
     3781 tgtcctttta tagtagttgg atgaagtttt aaattcatct gagaattttg aactactgct
     3841 tggtcccaaa tagttgcaga ttcaaataat gcatatactc catttttatc tgttcctgca
     3901 tatgcagaga ctgaaccatc tgaatatgtt gttccactta gtctaccatc tggagtagcc
     3961 aaggtagcag ctccctgtgg cccatgtgta gaaacagata tttggcaagg gtacatcttc
     4021 ttagcatata aagattgtgc ttcttcacat acttttgata accatacctc atagttttta
     4081 agaatattat ctgcatacaa atcattattt ccatatttag gtgcatcaag acaggcctta
     4141 taaatagcat catatttacc agtcttatct acttttactt ggtctgccat agaataattt
     4201 cctacttcta atgcatcttt aaatccaaaa ttatttaata tggcatcctt aatttcctct
     4261 attgtaaatg ctttgtcatc atatacaagt ttcttaagcg ctgcaaatga attgaccata
     4321 gtaacagtac cacaagtttc tacgtttaat gttgcattat aacgatatcc catatttcct
     4381 atatgttgac ctttgtctaa acagtctggc tttagtagtg aatttattat agaagtatca
     4441 aattttctcc atatatctaa ctcaatgtta tttgctcttt ctaaaacatt tattgcttgt
     4501 ttataataat ctttaaatac ttctatgact tcttcataag tatctaatgg cttattatga
     4561 ggctcaaata cttgtatatt catacgatag tctttaccat tcataagaac aagttctaaa
     4621 atttttggat tagctataaa gtgaactcca gagccagcag attgacctgc tccacctgca
     4681 atagagtacg tttttccgtt taaagttaac tgtttccaac accctggaga cgtttccaaa
     4741 catccaccta atgcaaatgc tcttgcttct tcaacagtca taccttcatc agcaaattgt
     4801 ttcatcataa atgttgttcc atttgagttg tttacccatg ctggataacc agaaccaagc
     4861 tttgtacatt cagcagcttt cattaagaaa tcttctggaa gcttttcatc atatagcata
     4921 gttaaactag gttgaggagt tctacatctc atacttgctt ctaataataa ctcttctagt
     4981 tcattagctc ctgtagaacc atcttctttt aatccaccta tagataaagt attaaatgta
     5041 ttacctgata gtactcctcc atttaccccc gctgaagcaa aacagtcaat acaagtgatt
     5101 tttattctat aaagctctaa taattcgata acctcttctt ttgtaattag tcctttttct
     5161 atatcttgtt cataccaagg atataaaatt tgaccaaaac gtcctataga cataccagat
     5221 atagcatcct catttactga tgctatatga attgtataag tcaactgaag agcttcccta
     5281 aatgtacgag gttgtttatg tgcaatatgt tctaatatct ccactaaatc tgagtattct
     5341 tttttagctt ctaaatctgt ctctatactt tctaaatatt ttgcatgttt tgcataattt
     5401 aaaatccaag tttgtaatcc ttcaataact tgaataacag cctcataaaa atatagacgg
     5461 tccataccaa taagcccatc tccagatgca tttcctgcaa ctgccttttt acattctttt
     5521 gccatttcta taattccatc taatccataa tttaaaggat aaaaataatt tataacttct
     5581 ctaccttgag gaagtgtata ccccgaatca aacatgcata taagtgtact cattaggttt
     5641 tcttttaaat cataatctgg catcattttt tcatagtgaa aacctaaatc ttcaacagac
     5701 tttccaaccc attcctttgc catttttaca agtacaggta cttcttcttt tctcattccg
     5761 aatttaccag ctattgaaac gacatttcca aaactttcag taacatttcc tccaccagac
     5821 ccaaattttg taagttcatc agctgtgtta ctggctaatt tctttgcctc ttctctcatt
     5881 tgttctcctt gagcaacaaa gaaactttca cttacccatg gcattggaaa agaacctcta
     5941 taatggcgtg tcttttgcat tactattttc tcaccaggta ttatatttgg tgtcatatgt
     6001 gaaaaagcag ctttaagagc tttagctctt ctaactactg gtatatcacc atctgatttt
     6061 ctatattctc tattatacca atatggaaat tccatatcag cagtagataa tgtattataa
     6121 tatacatcta ataagtcttc tgctctttta cttgcttttt tttcattaaa ttcacttaaa
     6181 ttttctttat tttgaaaatt tgatttaata ttttttgctt ccaaaatact tcttattttg
     6241 tcttctttac tttgactcat ttcttcccct ccttaatctt tctataatta gtataaaact
     6301 ttttaagaat gaaaaaaaag ggtatattac ccacttttta aaataatctt tctatagaaa
     6361 aaaataaact tgaagcaaat ctaagccaaa gacaatctgt atatcctaaa atttgttgca
     6421 attcattgga atataaagat tataaatagt tttacttcaa gttttaatat atactatatt
     6481 tatttttatt atatttaatt tcacttctac ttactttttt gaaacaatac ttttatattt
     6541 acaaattcat ctaactctac tgatttatct ttaaatttgg aactgcattt aaactcatat
     6601 catgcgtttt cccatttata aaattataat atcctgcaca ccctatcata gctgcattat
     6661 ctgtgcataa aattagagga ggatacttaa cagtaatacc attgtcttta gcaagctctg
     6721 ttattttagc tctaagcccc gaattagaag caaccccacc agaaagagtt atgatattgt
     6781 atcctttgtc ttttactgct tttagtgctt tagtagataa aacctctact acagcctctt
     6841 ggaaagatgc tgctacatct tcaactacta tttcttcatt tttcattctt tttccattta
     6901 gataatttaa tacagaagat tttaatccac taaaactaaa atcatagcta tcttcttcta
     6961 aataggctct tggaaattct atagcatgtt tatttccatt cttggccaga ttatcaataa
     7021 taggacctcc tggatagcct aaattcatcg ctcttgaaat cttatcaaat gcttctccag
     7081 atgcatcatc tcttgttttt cctaaaattt catacttgcc ataatctttt acttcaacca
     7141 agtgagtatg acctccagat actattaatg ttataaaagg aggttttaaa tccttatgct
     7201 ctatatagtt tgcacttaga tgcccttcta tatgatttac tcctacaagt ggaatattca
     7261 aagtatatgc aagtgcttta gcataagata gaccaaccaa taaagcacct actagcccag
     7321 gtccatatgt aactgcgata tggtctatat cattaaaacc tatatttgct ttgtctaaag
     7381 cttcttgaac aactatatca atattttcca catgttttct tgaggccact tctggaacta
     7441 caccaccaaa ttttttatgt gtctcgattt gagtagagat tatgtttgaa agtatttctc
     7501 taccattttt taaaactgat gcagctgttt cgtcacaact actctctata gctaatgtta
     7561 ttatatcact cataaactct acacctcttt taattggttc cacattataa ttgcatcttc
     7621 tttattatca ctgtagtatt cttttcttat tccacccatt ttaaatccat attttctata
     7681 taaattttgt gctattttat ttgatgctct aacttctaat gtcatagatg caatattatt
     7741 atctttacat aaatcaacta tgccttttat caacttgtcc cctatttttt tccctctata
     7801 gtcactatga acagccacat tatttatatg cccttcatca agtataagcc aaattcccac
     7861 atatccgaca atttttccat ttaacttagc tactatatac cttgctactt cattagacaa
     7921 ctcttttcta aaagactctt ttgaccaata atcttcaaaa caattttttt ctacttcaaa
     7981 aacttcatct atatcttctg tagtcatttc ttctattttt atatcatcta ctattagttt
     8041 tgtattatct tccattattt agcctcttca ttttttcatc atattgtact tcagcttgag
     8101 attttctgat atacatagga tttatcgtat aacaagtatg tacatctata tttttattat
     8161 atttatttaa tgctaaagag cataagcttc ctgcgttaga tacattatta gctggtgaag
     8221 gtatttttat attttttata ttctctaact tgtctttata tttgtataca gcttctccaa
     8281 ctattataaa ttcttcatta gttgatacaa tttcatctac taaagcatca aaatctacaa
     8341 catcaacacc atctaattct atcatctctc cattttcaaa cttgtattta gctgaataaa
     8401 cttgattttt ctgcgcatct aatatacaac atatctttct atcacagaaa tttatgttat
     8461 ttgctagaga ttccaaagaa tttactgcta ttattggtat attatttaca tgggccatag
     8521 cttttactgt agccatacct attctaagtc ctgtaaacga acctggacct atacatacag
     8581 ctaataaatc catatctttt atacttaaat cactcatact caacatattt tctatcattg
     8641 gcataagctt ttgtgaatga gtttttttgt tgtttactgt aaactcacat attaagttat
     8701 catcttcaac aactgctaca cttgctgcca tagaagacgt atccatacct agtattttca
     8761 ttatttaatc aactcctcaa ctatctcttt ataaaactct ccttttggag ttaatatcat
     8821 ttctcttcct tcatctttat atctcaattc tatattcaaa tattcttttg gaagtatatc
     8881 ttctattaga ttagcccatt ctattataca gattccattt gaatttacat attcatcata
     8941 accaatatca tacatttcgt cactactacc tattctataa acatcaaaat gatacaaagg
     9001 tatttttcct tcatattcat ttattatagt aaaagtagga cttgttatat aatcttctat
     9061 ccctaatgaa tctgccaaac tttgtgtcat tgtagtttta ccagctccta aatctcctac
     9121 taaacagata acactgcctt cttttaaaag tttacctaat ttatatccta tttctctagt
     9181 tttattttca ttttctaaat atatttttgc catcaattca tctccatttt gatataatcg
     9241 tcatgtgaat ttatatcact gcttaattaa ttatagtata agcctagatt attataaagt
     9301 acttgtcttt atcttttttt atctttcact attgatggct gtatctacac cttatataca
     9361 atcatcaata gcaattttta catactcttt tgctttatgg ttttgcttca atgcttcttt
     9421 acatattctc tcagattgct cttttacata cttaagagac ttatagttct cttttactgc
     9481 ttcaatacac attttttctg tttgattttt tatatacata agcatttttc cattttgtct
     9541 tacaatctct atacatatat cttctgtata cttatctatg taacttagta catctttaat
     9601 attatatttt ttatttaaat atcttatagc atttatacat atttcttctg tcttattgtt
     9661 gacaaattgt atacttcttc catcttgttt gacagcttct atacatattt tatcagtttg
     9721 tttatttaca tattgtaatg ccataccatt ttgtcttacg gattctatac acattttttc
     9781 cgtttgattt tttacatatt ttaatgcttt ataattttgc ttaatggcct ctatacataa
     9841 ttcttcactc tgttctctaa cgtatgcaag acacctgcca tcttttttaa ttgcttctaa
     9901 acatatcctt cttgtttgag tctctaaagc ctttagtata cttacaacat ctatattgga
     9961 agaatgactt attgctttta taataagccc ttctgtttta tttcgtacat acttaagtgc
    10021 attacaatct ttttttatag cttcaagaca caagtattct gtttgttctt ttacatattc
    10081 aagtgcttta taattatact ttattgcttc taaacataat tcttcggttt gattttttac
    10141 atactctaat gccttgtaat tctgccttat tgctaaaata cacataccat cagtttgatt
    10201 ttctatattt tttaattcac gataatcgta ttttaccctt tccatatttt caatatattc
    10261 attagtgata tactttaaag aatctcctaa ttttgtatca taaataccaa tatgaatatc
    10321 tttccaatta ctattaaaat aatacccttt atcatctaaa aataattttt ttattttttt
    10381 agataaatgc atgaatatac acccctatca ttattaaatt agttattatt aaaagtttga
    10441 ttattaatct ttatagtttt ttaataataa ctttattttt tgtttatta
//
LOCUS       DAKBNN010000138        10316 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000138 DAKBNN010000000
VERSION     DAKBNN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.139"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            288..1712
                     /locus_tag="OUB02_002783"
     CDS             288..1712
                     /locus_tag="OUB02_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCT8886526.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIDEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1747..3876
                     /locus_tag="OUB02_002784"
     CDS             1747..3876
                     /locus_tag="OUB02_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT8886527.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSRIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3906..4328
                     /locus_tag="OUB02_002785"
     CDS             3906..4328
                     /locus_tag="OUB02_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8886528.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4347..5498
                     /locus_tag="OUB02_002786"
     CDS             4347..5498
                     /locus_tag="OUB02_002786"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCT8886529.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5604..5705
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5888..6460
                     /locus_tag="OUB02_002787"
     CDS             5888..6460
                     /locus_tag="OUB02_002787"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCT8886530.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6524..7174
                     /gene="fsa"
                     /locus_tag="OUB02_002788"
     CDS             6524..7174
                     /gene="fsa"
                     /locus_tag="OUB02_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCT8886531.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7326..10250
                     /locus_tag="OUB02_002789"
     CDS             7326..10250
                     /locus_tag="OUB02_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886532.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4229 a         1008 c         1938 g         3141 t
ORIGIN      
        1 tattagtata tccaaaagta catttaataa atttatttaa ctgaattatt attttcattt
       61 ctattaaatt taaatatttt ataaaaatag tattatttac aaaatcatat actccaaaat
      121 actacataaa taaaattaaa caaaaactaa aatgacaaat gtcattatct aaagtgacat
      181 agaaacggtt tcttaaagtt gttactataa aatataaact ctataataaa agtataacaa
      241 aagggaatca tatcgatata gaatataatt caagaggagg taaaattatg agtagttcta
      301 atcaatcgac aataaagaca tctatacagc gagtagggaa atttttaagt ggtatggtaa
      361 tgccaaatat aggagctttt atagcatggg gtcttataac agcattattt ataccaacag
      421 gatggatgcc taatgaaaaa ttgagtacta taggtgaccc aatgataaaa taccttttac
      481 cactattgat agcttatact ggtggtaaag caatagcagg acaaagaggt ggcgtaatag
      541 gtgcagcagc agctatggga gtaattgttg gtgcagatat accaatgttt atcggagcaa
      601 tgataatggg accatttgca ggatgggtaa ttaagaagtt tgataaattt gtagatggaa
      661 agataccaac aggatttgaa atgttagtaa ataacttttc aataggtata ataggtatgc
      721 ttcttgcaat actaggattt tatgcaattg gaccagcaat agtagcaggg acagctctta
      781 tagaatctgg agttcaattt atagttagca aaagtttatt gcctctagtt tcagtattta
      841 tagaacctgg aaaagtatta ttcttaaaca atgcaataaa tcatggtata ttagggccta
      901 taggtatagc tgaggcaaaa gaagcaggta aatctataat gttcttacta gaaagtaacc
      961 ctggaccagg tttaggagtt ctattagcat attggatgtt tagtagagga agtgtaaaac
     1021 aatcagcacc aggagctgta ataatacatt tctttggtgg tatccatgaa atttacttcc
     1081 catatatttt aatgaatcca gttttaatat tggctacaat ggcaggtgga gcagcaggaa
     1141 tattgacttt ttcaatatta ggagctgggc ttgtagcagc accatcacca ggaagtatat
     1201 ttgcacttat ggcactagct ccaaagggag gattacttcc agtattagca ggagttgcag
     1261 tggcaacagt tgtatcattc ttagtggcag caccatttgt taagagagct tcagcaaatc
     1321 aaagtgaaga agacagtact tctttagaag aagctaaagc aaaaatgagt gatatgaaat
     1381 ctgcaagtaa gaactctgag aaaaatattg acgaaaaaca gcttgaagta aatgagatta
     1441 agaagattgt atttgcatgt gatgcaggaa tgggttcgag tgcaatggga gcttccagat
     1501 ttaaaaatag aataaaaaac ttagacttaa atatagaaat tacaaattca tctgttgata
     1561 atcttccaga tgatacacaa atagtagtaa ctcacaatac attagttgaa agagttgcta
     1621 agaataattc aagtgttgag atagtttcaa taaacaattt tttaaatgac ccaaatttag
     1681 atacattgtt taaaagatta gaatctaaat aaaaataaaa agtaccacta attaggagtg
     1741 gataatttga aaaaaaagaa gattacatct aggcaaaaaa agataatact aatgatagta
     1801 gaaaattcaa agaaaaacat tccaataact atttctgaga ttgcagggac tttagagtta
     1861 agttcaagaa ctgtacttag agatatgtct ggaattgaaa aatggtttga tgaaaatgat
     1921 tttaactttg taaaaaaacc aggagtggga ttgattttag aggaaaatat agaaaatcaa
     1981 aactttataa ttgagttgtt agaagaagaa aaaatagaaa aagaatacag caaggaagaa
     2041 agaaatttaa ttatattatc aaagctacta gtttctaacg agccagtaaa atcctattat
     2101 tttgcaaaaa tattaaaggt atctgaaggg gtactgaata atgattttgc cttagcaagt
     2161 aaatggttgg aaagatttga tattgagctt gttagaaaac cagggcttgg tgtctattta
     2221 aaaggtcaag aaaaaagctt tagagaggca tatgtaaatc ttatatacga ttcattcaat
     2281 gaaaaagaaa tactagatat ggtcagaaat ataagtgaaa atatacaaac agataaagca
     2341 atagaaattt taagtgaaaa tagactttta aatttaatgg acaggtgcat aataagaaaa
     2401 gttgaagcta cattaacaaa gaagctttca gatttagatg taaatttagc tgatagtgca
     2461 tatatagggc ttgtagttca tatttcatta gccttacaga ggataaaaaa tggtgaaaat
     2521 ataactatgg ataaagagtt tctaaaagaa ttatctataa cagaagagtt taaattagct
     2581 aaagaaatcg taaaaggtat ggaaacagat tttaacatgg atataccagt agatgaggtt
     2641 ggatatataa ccatgcatat aagaggtgca aagcaaaggt catcttcaaa ccataaggct
     2701 ttaaacttag atgatataga aataatggaa attacaaata agatgataga tttggcggaa
     2761 gatgaattta aaataagttt aaaaaatgac gagaggttat ttaaagacct agcaaatcac
     2821 ttagggcctt ctataaatag attgaatatg ggattagaaa taagaaatcc tcttttagat
     2881 gaaataaaaa gcaagtattc atatgcctat gatggagttg aaaagatttc tagaattata
     2941 aaagataaac taaatataaa ttcaatacct gaatcagaaa taggatatat tgcaatgcac
     3001 tttgcatcag ccatagaaaa aaatcttatg atgaatacta atataaatat tgttgtggca
     3061 tgtcccactg gaataggtac ttcaagattc ttatcaacta agattgaaaa taaatttcct
     3121 aatttaaata tacttgaaac catatcagct ataaacatag atgaagagta tttaaaggaa
     3181 aaagatgttg atttaatagt gtctacagtt gagttaaata caagtttaaa ttatatatgt
     3241 gtaggtccat ttatgagttt agatgatgaa cagataatca aggaaaaaat aaaatctata
     3301 gctcaaaata aattaatcaa tttaaatgcg aaaaatgata ctaagagtaa gaataaagta
     3361 tatgaacaga taacagaaag tatgaatata ggaaaagata ttcttcaatt tttagaggaa
     3421 attagatttg aaaaatttga aagtaaagat ttaagtgagc ttattgaaga ctcatcaaga
     3481 atatttgcaa aatctagtga agatataatc tctataaaag aatcgttaaa agaaagatta
     3541 aaaatctcaa ttccatatat agaagaatca aaaattctat tgttacattg tatgagtgaa
     3601 agaatagata ttatgaagtt atcaataata aaactagaga atagaatcgt gctagattca
     3661 aatgaagaaa tcgacaatgt agtttttatg ttacttccta aaaattcacc aagttatcaa
     3721 agacaaataa tgagtgaaat aagtggttca ttaatagaca atataatttt tactaataag
     3781 ataaataaat tctctataga agagatggca ttagaggtaa aagatatagt ttttaatttt
     3841 tatacaaaca gattaaaagc atttatagat aaataaaatt aatattaaaa aggaaggttg
     3901 atactatgag taaagtattg aatgagaata atatattttt agggttagat agtgtttcaa
     3961 aggaagaagc tataacttta gcaggaagaa aattagttga aaatggatat gtaaaagaag
     4021 aatatatacc agcaatgtta gaaagagaaa aagtaatgac tacatatatg ggaatgggag
     4081 ttgctattcc acatggtgta aatgaagcaa aaaaagaaat attatcttct ggaatagtta
     4141 tattacaatt ccctaatggt atagattttg atggagaaaa agcatattta ttaataggaa
     4201 tagctggtgt tggagatgag catttagaaa tactatctaa tatagcagta gttttagatg
     4261 atgacttaac agaaagacta aaaaattcaa atgacaaaca agcttttatg gaagcttttg
     4321 ccaactaatc ttaggaggga tttaaaatga aaaaggcaat tcagtttgga gcaggaaata
     4381 ttggaagagg ttttattgga ggcttgttag ttaagtcggg ttatcatgta gtatttgctg
     4441 atgtaaatga ggatatatta aactcaataa acaaagataa aaagtatact atacacataa
     4501 gagatgtaga atgtatagat gaagtaattg ataatataag tgcggtttca tctataaaag
     4561 aagaaataat agatgagatt gtacaggctg aaataataac tacagcagtt ggacctttag
     4621 ttttgactaa aatagcctct acaattgcaa aaggtataaa ggctagaaaa gaaaagggat
     4681 taacaagtaa tctaaatata atagcatgtg aaaatgcaat atacgcaagt tcttctttaa
     4741 aagaagaggt acttaagtat ttaaataagg aagaagtaga atatttagag atgtatgttg
     4801 gttttccaaa ctgttcagtt gatagaatag ttcctcctgg taaaaacgaa aatcctcttg
     4861 atgtaacagt tgaaaatttt tatgagtgga atgttgaaaa acaaggattt aaaggggaaa
     4921 taccaactat agttggaatg aatttagctg ataatcttat ggcttatata gaacgtaaat
     4981 tgtttacact gaatacaggt catgctataa cagcttatat aggatattta aaaggatata
     5041 agactataga agagagtata aaagataaat ttatctgtga tatagtcaaa agtgcaatgg
     5101 tagaaagtgg agaaggattg attaaaaaat acaattttga ctcagaagtg cactataaat
     5161 atattgataa aattttaaat agatttaaaa atccatatct taatgatgat gtattaagag
     5221 tcggaaggga accattaaga aaactaagtg ataaagatag attgattaaa cctttaatga
     5281 cagcaaaatc atatggatta tcagttgata atttgattct tggaataggt gcagcgcttc
     5341 attataataa tagtgaagat actcaaagtg tagaattaca agagttgata aagtctattg
     5401 gggtaaaaaa agccgttgct aaaatagcta atataagcaa tgacgaagaa cttttaaata
     5461 atatcgaaaa aagttatata tttatgaaaa atttataaat ttacttgaaa aaaagtagtt
     5521 ctgataaagt aactataata tattattcta cacagtttga attatttgtt gaataataga
     5581 gaattataat atataattaa aaggaataaa ttaatatatt tacctgtata tgtttgataa
     5641 tatggttcaa atgtttctac taagctacca ttaatagctt gactacagga gtgaagaaga
     5701 tatagtttat acttatttta aattatatat taagttaatt actttgaatt ttaagctcct
     5761 gtctatgata tagacaggag cttttttagt attttaatat aaaatactta caagaataga
     5821 tagcaaaatt agaacaaata taattttttt attaatataa ttttagaaag ggctaggtgg
     5881 aagttcaatg aaggctttac aagaaaagat attgagagaa ggcagtgttt caggaaatga
     5941 tatactaaaa gttgatagtt ttttaaacca tcaaattgat gtagcttttt taaatgaaat
     6001 aggaagagaa tttaaagaga gatttaaagg agaaaaggtg gataagatat ttacaataga
     6061 ggcttcagga atagctatag catcaattgt ttcacagtat tttgataatg cacctgtagt
     6121 atttgcaaaa aaatcagaat ctaaaaattt ggataaagat gtatatgaaa ctaatgtata
     6181 ttcatttact aaggcaagag agtattcagt taaagtttct aaaaagtaca taaacaaagg
     6241 cgaaaatata ttgatagtgg atgatttttt ggcaaatgga agagctgctc ttggacttaa
     6301 agatttaatt gagcaagcag aagctaactt agttggagta ggaattgtca ttgaaaaagg
     6361 atttcaagct ggaggagcac ttttaaaggc taatgatgta agattagagt ctttggcagt
     6421 agttgaatct atagataatg gaactgtaaa atttaggtaa tttatgatat tatatacttg
     6481 tgtttagcac atattttaac tatgtaatta tggaggattt tttatgagat tttttataga
     6541 cacagccaat attgaagaaa taaaagaagc aaatgattta ggtgtaatat gtggtgtaac
     6601 tactaatccg tctctaatag ctaaagaagg tagagatttt atagaggtag ttaaagaaat
     6661 aagtgaaata gtagatggac caataagtgc agaagtaata agtttagaac ataaaggtat
     6721 gatagaggaa gcagaaaaat tatctaagat acataaaaat atagttataa agatacctat
     6781 gacagcagaa ggtctaaaag cggtgaaagt actatcaagt aaaggtataa agactaatgt
     6841 aacgcttata ttctctgctg gacaagctct tttagctgct agagctggag ctacatatgt
     6901 aagtcctttt gttggtagat tggatgatat atcacaaaat ggtcttgatt taattgaaga
     6961 aatcgttgat atatttagtg taaatagtat tgaggctcaa ataatagttg ctagtgttag
     7021 aaatccaata catgttttac aagctgctag aatgggagca gatatagcaa cagttcctct
     7081 taaagttata aaccaaatga ttaagcatcc tttgacagac aaaggaatag atagctttat
     7141 gaaggattgg gaaggtgctt ctttaaaatt ataattataa actacgcttt ataattatat
     7201 taaagaggtt attatggaaa ttctaagtaa taaggctagg ctattttgcc tagtcttatt
     7261 ttggtgtata atatattgaa acatattatt gaaatagatt aattttttaa gtgaggaggc
     7321 gaaaaatgga taaaatatta agagagatac aaagagaaga taaaaaaaat ccatatactg
     7381 accaagaact tgctgaaatg ttaaatgtag ctaggtctga agtgatatca gttaggaaaa
     7441 aaaataatat tttagattcg agagaaagaa gaaaaaaaat attaataaaa gatatatcaa
     7501 aaattgtaag tgaaaatcct caaatgtctg aaagaaaaat aactgaaata ttaatagaaa
     7561 atggatataa tatatcgaga agtgcagttt caaaattatt aaaagaggaa aatttaaatc
     7621 agtcaaagat tgggattaag gataaagacc cagcatcatc aggaaagtat gtgaaggtga
     7681 gtaaagttgt agaagaagat ggatttgaag aattgatagg tataaaggga agtttgaaag
     7741 aaaaggtaaa tctagctaag tcagcaataa tgtatcctcc taatggcctt catactataa
     7801 tatatggaga aacaggagta ggtaaaagtg aattagctac atgtatgtat aaatatgcta
     7861 taaaaaacaa cattaaagaa gagaacagcc catttatagt gtttaactgt gcagactatg
     7921 cagaaaatcc taatctattg atagctcaac tctttggggt agtaaaaggt gcatatacag
     7981 gagcagatgc aaatagagaa ggtcttgtag aacaagctaa taatggaata ttgtttttag
     8041 atgaaattca tagattgcct gctgctgggc aagaaatctt attttcatta atagatagag
     8101 gagaatttag aaggttagga gaaagtagtt ccaatagaaa ggcaaatgtt ctcataataa
     8161 gtgctacaac agaaaatcca gattctaatt tacttcaaac atttagaaga cgtataccaa
     8221 tggtaataaa tttacctaat atgagtgaaa gaccaaaatc tgaaagatat gaaataataa
     8281 taaagttttt tgaaagagaa gcaaagcgag taaacaagaa ttttataata actaaagaag
     8341 ttatgtgtgc acttatgaat tataagtgta ctggaaatat aggtcagtta aagagtgata
     8401 tacaagtaac ttgtgctaga gcttttagta aatcgatatt ttctagcgat aaggtaatta
     8461 tagatttaga tagtttaagg gattatataa aaagtggata ttatgattgt aattgtaaaa
     8521 atgaaaagta ttctgagttt tcagttgagg atatatatat agatattagt gaattggtga
     8581 gtggaaaaaa tagaatttta tctgataatg agataagcga gatatataat tatgcagaaa
     8641 aagagcttaa ggttttggaa agtaagtgct tttcagaaga agagttaaaa aatgcattta
     8701 tagaaaagtt agataagaag tttaatgcta ttaaaaacaa taaaaattta tctgatagaa
     8761 gaagtcgaat agactgggga ggacaattaa aagagtctac actggagata atggataagg
     8821 taattgtatt tattaaggag cagtttaagc atgtaaatca aggtctgtac ttggctctag
     8881 caattcatat tgagcatgct ataggtagga taaaagatgg aaaaactata ataaatccga
     8941 gtttagataa aataaaagca tcaatgccaa tggaatatga gttgtcaaga tatataatgg
     9001 gtatagtaga agatttgaca caagtaacat ttccagaaga tgagcttgga tatttggctt
     9061 attatataaa taaattttgt tataatgagg aaagtataaa agataaagtt aaagtagtaa
     9121 tagttacgca tggtaaagtt ggaattgaaa tgtctaaagt tgtaaatcat attttaggta
     9181 tagaatgtac actaggtata gaaatagcac ttacagattc accatcagag gggatagagc
     9241 atgtattaga agaacttcaa aagatagaag ctagaaaagg tattctagtt ctaattgata
     9301 tgggttcttt ggtaattctt ggtgatgagg ttgaaaaaag gcttggaatt agatgtaaaa
     9361 ctgtaaatag agtagataca ttattggcaa tggaagcagg aaaacttgca accattgaag
     9421 ggaaaagttt agatggtata atagctgatt taaagaaaaa taagaattat gctatgataa
     9481 atacaaataa atttagttac agaaaaaacg agtatggaaa gaaaaatgta atcattactt
     9541 tatgtttgag tggtgtaggt acagctttaa atttaaaaga gcatatagaa aaacaaatta
     9601 aggaatatga cactttaatt gaagtaaagc cagttgcttt tttaaataat aatttagaag
     9661 aagagttaaa tgatattgaa tctgaatata atattattgc tatctgtgga actattgata
     9721 tagattataa aaacgtgcct tttatttctt ataatgaggt acttggtaaa aatggtatta
     9781 ataaagtatt agagcattta aatagtaaac aaccagcttt aaatattgaa aaccagttga
     9841 ataatttaat acatgaagat ttgattttat acaattttga aggaatttca aaggagtata
     9901 taattgatac tcttgtttca aaacttgaag aaggtggata tgttgactca aagtatatat
     9961 taagtgtata taaaagagaa gcaatgggaa gtgttgtaat ggcctctaag gtagccgtgc
    10021 cacatggact tccagaaaac gttataaagc ctgcaatagc aatcgcgagg ttgaataaac
    10081 ctatagtatg ggacaataaa tttatggtgg atttggtggt actactggca cttaaagaaa
    10141 ataataagaa agaaatacga agcttatttt caaagattaa tgaccagcat actttagaag
    10201 ttttattaaa tactgaagat aaaaatgaaa taaaagaact tttaagctaa ttattagtac
    10261 cgagaagaaa gacaaaaaaa tttgctttag tataaaattt aaaactactc aattgt
//
LOCUS       DAKBNN010000139        10157 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000139 DAKBNN010000000
VERSION     DAKBNN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.140"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     repeat_region   473..1030
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=473..501
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1977..2144
                     /locus_tag="OUB02_002790"
     CDS             1977..2144
                     /locus_tag="OUB02_002790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886533.1"
                     /translation="MWYVNFSASSTMLYSSNGFILTMWYVNAHLTSSLRVLSASFILT
                     MWYVNFVQLLS"
     gene            2582..3097
                     /locus_tag="OUB02_002791"
     CDS             2582..3097
                     /locus_tag="OUB02_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8886534.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            3162..5555
                     /locus_tag="OUB02_002792"
     CDS             3162..5555
                     /locus_tag="OUB02_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCT8886535.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIDSITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVSNVTLGAILIINGLGWVTNATLNVANVFAQNWSIIAPIIYG
                     VIAAIAIYKGVLLASTIATTVASFVNSLYAVAAYKSCAALAAQEFAIFGKISAQTMEA
                     LVTAQATAAQWGFNAALLSCPIFWIIAGIIAFVVVVFVAVAAVNKFSGTSLTVLGAIV
                     GAVFAAVAFIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DAVNNAGNALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNKWVGDAPEKIKLDRMGYKDIGAEFQKGNALGTKWQNAITDKFKDTFDINK
                     MLEDAKKKLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            5571..6266
                     /locus_tag="OUB02_002793"
     CDS             5571..6266
                     /locus_tag="OUB02_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT8886536.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVPAPPKKTNETGRPSVKIE
                     PKRVDSVNAPSNKTYTVKSGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKKVP
                     KYTIYKGQVLRLV"
     gene            6259..8151
                     /locus_tag="OUB02_002794"
     CDS             6259..8151
                     /locus_tag="OUB02_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT8886537.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRFFKNK
                     DTYVLENHTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRLTLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQMFETVDKNMNEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            8165..8425
                     /locus_tag="OUB02_002795"
     CDS             8165..8425
                     /locus_tag="OUB02_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HCT8886538.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            8430..8849
                     /locus_tag="OUB02_002796"
     CDS             8430..8849
                     /locus_tag="OUB02_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HCT8886539.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            8850..9896
                     /locus_tag="OUB02_002797"
     CDS             8850..9896
                     /locus_tag="OUB02_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCT8886540.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIATERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPNMKGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWENI
                     KSIAKEAVDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNLT
                     IDSNSIVVRGEVVG"
     gene            9889..>10157
                     /locus_tag="OUB02_002798"
     CDS             9889..>10157
                     /locus_tag="OUB02_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCT8886541.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLP"
BASE COUNT         3914 a         1155 c         1919 g         3169 t
ORIGIN      
        1 ataataatcg aaagtgcgaa gggtgattat tttcatatta aacgccaaat tccaaataag
       61 gaaggaggtg aaattatatg ataggttttt tattaagcat actagctggt gttatatcag
      121 cttatattta tgacaaaata aaaaatcacc cagacgccaa taagggtgat ttaaaaaaat
      181 aatattttca cttaacaact gaaaataatc actctttgta ggagtaaatt atttccttgc
      241 ttttattata ccacaaattg gtacagatat tcaaaaataa tatttttatg atataataaa
      301 aatgtagaga ttttgcagtg agcgatattt gttacaaaat atggcttaac acttgaaatc
      361 taagatgttg agggtgtgtg ataaatgtta tcaattgcac tactcatggt tcactgcaaa
      421 tttgagagag atgcgtatgt gtaggtattg gaaatgccaa gtttattttg gggttttaga
      481 ttaactatat ggaatgtaaa tttatttttt tgttatcttt taattcaaca gcttctttgt
      541 tttagattaa ctatatggaa tggaaataca aaattattta aaacaaatcc agcagaaacc
      601 ttagttttag attaactata tggaatgtaa attcagaatt aactaatgtg aattttttag
      661 acatgtcata gttttagatt aactatatgg aatgtaaatt gatagaattg aaagctactt
      721 cgtcacttga taaagtttta gattaactat atggaatgta aatgaaaaat acactattta
      781 taataaatgc tgttaaaccc ggttttagat taactatatg gaatgtaaat tttatcattg
      841 tgtaatgctc tttagtaata gaacctagtt ttagattaac tatttggaat gtaaatatag
      901 ttataatcaa tattacttcc aaggacagta gaaggtttta gattaactat atggaatgta
      961 aattttactg catttatacc atctacacct tttattttat tgttttagat taactatatg
     1021 gacttaaaat taaaaatact gaaaaacact tacttttatg gtaggtgctt ttttatatta
     1081 aaatctatgt acttaattga aatatttggt aaaatatgta agaattgtat gatataataa
     1141 ttgtagcaag gaaaataata attgaaaagt gctaagagtg gttatttcca tatttgaata
     1201 ccaaattcca taacggaagg aggtgtaaca gtatggtgat aaatttttta ttgagtatac
     1261 tggctggtgt tatatcagcc ttcatgtatg agaaaataaa aaaccactca aaggccaata
     1321 agagtggttt aaaaaagtaa ttctttaaat caattttgat ggaaatagct actcttgtat
     1381 aaagtaaatt atttccttgc ttttattata ccacaaattg gtacagatat tcaaaaataa
     1441 tatttttatg atataataaa aatgtagaga ttttgcagtg agcgatattt gttacaaaat
     1501 atggcttaac acttaaaatc taagatgttg agggtgcgtg ataagtgtta tcaattgcac
     1561 tattgcccgc tcactgcaat tttaagagta ttgtatatat gtaggtattg gaaatgctaa
     1621 gtttattttg gggttttaga ttaactatat ggaatgtaaa tatccattct ttattgtcta
     1681 tatctataat tttaacctgt tttagattaa ctatatggaa tgtaaagcaa caagaacaac
     1741 agttgatttt accttttcta gccaacgttt tatattaact atgtggtatg taaattttgt
     1801 tataagaata tcgccacctt ccttttctgg tgttttatat taactatgtg gtatgtaaat
     1861 atctttatta agagggctat cataaagagg tgcatagttt tatattaact atgtggtatg
     1921 taaatatgac tctattttat tttttatggt cctacaattt ttgttttata ttaactatgt
     1981 ggtatgtaaa tttttctgca tcttctacta tgttatattc ttctaatggt tttatattaa
     2041 ctatgtggta tgtaaatgca catttaactt caagtcttag agtattatct gcaagtttta
     2101 tattaactat gtggtatgta aatttcgttc aacttttatc ttagcttctc ctgctacttg
     2161 ttttatatta actatgtggt atgtaaatgt gctatttcca actttttttt caagttctga
     2221 atactgtttt atattaacta tgtggtatgt aaattagtta gtcccatatc gttatggtac
     2281 tgcattaacg cgttttatat taactaagtg gtatgtaaat agtgtaaaag gcattttttc
     2341 aaattgtgca ttggtttggt ttatattaac taagtggtat gtaaaggcta atgtttgagt
     2401 atttaaaagc atctgagcaa acaattttat attaactatg tggacttaaa attaaaaata
     2461 attcaaaaac acttacaaat gagtaagtgt ttttttaatg aaaggaggtg ataataatgt
     2521 aaaaatttta cgtatatagt ataatagtgc tataaaataa gtattatgtg aggtgattgt
     2581 tgtgttttgt tcaaattgtg gttatgagat aactggtgca ggcaaatttt gctcaaattg
     2641 cggaacagct acattagcag ataaagttaa caatgatgat ttatttataa atgttcatgg
     2701 aaaagaatta aatctgacta atatttataa agaaactaaa ggagataaaa tcttagcaat
     2761 tgatattgca atgaagttac tagggctgga cataaaagag tgtaaaaata ttatatatcc
     2821 agcttttaaa gaattaagtg aaaaaataaa tatcgaagag gaaaaagaga tattgaggga
     2881 agatgagtat aaacaaacta atgtacttga agatgatgtt gctcgttgcc ctaggtgtgg
     2941 ctctgtttca ttgtctgctc ataagaaagg ttttggcata ggaaaagctg tagcaggggc
     3001 tactatagca ggaggcattg gtttagtagc tggaaattta ggagcaaaga aagttagagt
     3061 tacatgtttg agctgtggta aacagttttg ggcataaata ataaacactt actaatgtag
     3121 gtgttttttt atatggaaat ttatgaaagg agagtgagga aatggctaca atacaaactt
     3181 caataaagat tttcgacgga atgacaccag catttcgtaa tatgactaat tctattaata
     3241 caacaattaa tagtttagag agattgcaag gcagattgaa taatccactc aatgcaggta
     3301 atatacaagc ttctcaacaa agtttgaata acatagaaag tattctcaca aggatagaac
     3361 agaaaattgg aagaaataca aatgaacagg aaaactttaa taataaaata agacaaggta
     3421 gtgaagcagg ttctctatta gtgtctaaat taaaaagtat tgctgggata tacattggaa
     3481 taaaaggaat agatagtatt acaaaagcag cagatacaat tgcaagtaca aaagcacgtt
     3541 taaatctaat gaatgatggc ttacagacaa cagaccagct taataaaatg atttatttgt
     3601 cagcccaaag tgcaagagct agttatgcag atacagcagc acagattgct aaacttggaa
     3661 tacttgcagg agatgctttt ggaagttcag cagaggtgat aaagtttaca gaacttatga
     3721 ataaagcttt tgtaattgga ggaacatcag caaatgaagc tagtgcagca atgtatcaat
     3781 taacacaagc catgggtgca ggaaaacttc aaggtgatga gttccgttcc ataatggaaa
     3841 atgcaccttt attagccact aaaatagctg atgcaatggg aaaaactaaa gaccaattga
     3901 aggaattatc aagtagcgga gcaataacag cagatgttat aagaaatgca ctgtttaaag
     3961 cttctgatga gatagaaaag aaatttgcaa gtatgccaat cactttttct caagctctca
     4021 caatgatgaa aaatgatgct tatatgatat tttctgagac tctcagtaag ataagtggag
     4081 ctttgcaaag tgtacgtttt agtgagattg ttgtatctat gcggaatgtt atgattgcaa
     4141 tatcttcaaa catttatgat acattaaata ttataaaaaa tatattaaat agtgattttt
     4201 tttcagattt tgttagcaat gttacattag gagctatact aattatcaat ggactgggat
     4261 gggttactaa tgcaacacta aatgttgcta atgtctttgc tcaaaattgg tcgattattg
     4321 ctcctattat ttatggagtg atagcagcaa ttgcgatata taaaggagta ttacttgcaa
     4381 gtacaattgc cactacggta gcatcttttg taaattcatt gtatgcagta gcagcgtata
     4441 aatcttgtgc agctttagca gcacaagaat ttgcgatatt tggaaaaata tctgcacaaa
     4501 cgatggaagc tttggtaaca gcacaagcaa cagcagcaca atggggtttt aatgcagcat
     4561 tattatcctg tccaattttt tggataatag caggtattat agcatttgta gtagtagttt
     4621 ttgttgcagt agcagcagta aacaaattct caggaacaag tctgactgta ctaggagcaa
     4681 ttgtaggtgc agtatttgca gcagtagcat ttatacaaaa cataatgata tggctattta
     4741 atagatgcgt agatgtaaat gaagggattg caaatggatg gaatcaatgt gtatatctaa
     4801 tgaaacaagc tattgcaaaa ggtgtaatct ttataattga gaaaatggca tcattaaatg
     4861 atgctgtaaa taatgctgga aatgcacttg ggaaagcttt catagatggg gcaaatatcg
     4921 caatacgagg tgtaaacaaa ttaattgacc taataaataa aataccaggg ataaatattg
     4981 gtaaagttgg agaagcaaca tttacgcctg tcaaggcaga taatagttac atcaaacaac
     5041 agattgacag tttaaataaa tgggtaggag atgcaccaga aaaaataaaa ttggatagaa
     5101 tgggatataa agatattgga gcagaatttc aaaaaggaaa tgcacttgga actaaatggc
     5161 aaaatgctat aactgataaa tttaaagata cttttgacat taataagatg ctagaagatg
     5221 caaagaaaaa attaggatta gacgatttgt ggaataaaca aaatccttta aacaaccttg
     5281 gtggatttgg tggagattta ggaaaaaatg taaaagacac agcaggaaac acagccaaaa
     5341 tggctaaaac aatggataaa agtcaagaag acttaaaata tcttagagac attgcagaac
     5401 aggaaacaat aaaccgtttt acaggagtca acattaaaat tgacatgaac aatacaaaca
     5461 acataaatag tgaggcagat gtagatggaa tagtaaatgt actaacagaa aaattaaacg
     5521 acgctatggt tgtatcagct gagggaatag tttagagagg agggatataa atggcttatg
     5581 acttttattt agatggagta caattaccaa tacctccgcc aaagttagag attaaagtta
     5641 caaataaaaa caagacagtt gatttaataa atgttggaga agtaaatata ttaaaaaaag
     5701 aaggattatc tgaaataagt tttgaagcag aatttacaca taataaatta cctttttgta
     5761 gaggtcaatt tagagatgtt caattctttt taagtaaact agaattacta aaaactgatt
     5821 gtaagccatt ccaatttatt gtctcgaggg aaatgggtgg aaaagtacta tttaacacta
     5881 atatgaaagt gtcattagaa gaatataaca tagtagaaga tgcagagaat gcctcagata
     5941 caaaagttgc aataaagtta aaacaatata gagattactc aactaaaaag ttagttcctg
     6001 ctccacctaa aaaaactaat gagactggta gaccaagcgt aaagatagag ccaaaacgag
     6061 ttgattcagt caatgcacca agtaataaga cttatacagt caaaagtggt gattctcttt
     6121 ggtcaatttg tcagaagcaa cttggtaatg gttcattata taagaaggta tatgagttaa
     6181 ataaaacaat gatggataag gctaacaagg gtaaaaaagt acctaaatac accatctata
     6241 aagggcaggt gttgaggctt gtctgatgat ttagttctgg cgaatgatag agatataagg
     6301 ctagttattg cacattggga agatttctac gaacctgctg tcattgatgg tatcacatgg
     6361 gaaatagaaa gacgaggaac accatctaaa cttgaattta caatagtcat ggatgatata
     6421 ttagagtttt gtgaaggtaa ctctgtaaga ctatattaca aaggtgtagg tatattctat
     6481 ggatatatat ttcagaaaaa gagagataaa gaaaatcata tcaagatagt tgcctatgac
     6541 caattaagat tttttaaaaa taaagatact tatgttcttg agaaccatac tgcaagtgaa
     6601 cttgtaaaga tgttagctaa agattttaaa ttaaaataca atgtcataga agatacaaaa
     6661 tataaaatat cgagagttga agaaaataaa acactctttg atatggtctt aactgcacta
     6721 gatgatactc taagagagaa aaaggaaatg tatgtactct atgatgattt tggaagatta
     6781 acattaaaga atgttgcatc aatgaaactg gatactgtca tgaacaatga tgtcatagag
     6841 gactttgact ataattcaag tatagatagt gatacttata caaaaatcaa acttgtaaga
     6901 gacaacgagg agtcaggaaa aagagatgtg tatattgctc aagactctac acatatgagg
     6961 agttggggaa tactccaaat gtttgagaca gttgataaaa atatgaatga agcagagata
     7021 aaacaaaagt gtgatatact tctaaaacta tataataaga aaactaagtc attaagttta
     7081 aaaaatgcac ttggagatat tagagtgaga gcaggttgtt tagtacctgt ttttttaaat
     7141 ctaggagata ttaaacttca aaattatatg ctagttgaga aagtaaaaca tacttttgaa
     7201 aataacagtc actttatgga tttgactctt gttgatggag atgaatttgc ttcttattct
     7261 tcaagctcat attcaagtgg aaatactaac aataaggatg aaaagaaaaa tggtcctgca
     7321 caaagtatta cgaaaaaaaa tacaggtaaa aaagttcctg ctatatttac tgcatattat
     7381 ccagggaaca atgcaatgga aggcggaaaa acagattgca atggaaagcc acttgatgta
     7441 aaatcaagaa ctgttgctgg tccaatgaat cgagaaggag ttaagaaaac ttggtatact
     7501 gatgattttc taaataaaca tccagttttt gaatatggag ataaagtaaa aattatactt
     7561 cctggtactg cctatgacaa caaagtatat acagttaaag ataatggagg aagaatatat
     7621 gttgaaacaa acggaacata tcatatagat atactattag ctaatgctag tgaatgtaaa
     7681 aaatttggta gaaagaatgg ctatataatt ataggtggag atgaagaaca aacatatcaa
     7741 gttgaaggta ataaccaaag taatacaaat aacaattcta aagaagataa attaattagt
     7801 atagcaaaaa gtaaactggg ttgtaattat gtgtatggag cagaaggtcc taataatttt
     7861 gattgcagtg ggtttactca atggtgttat aaacaaatag gtataaaaat tcctcgtact
     7921 gcttctgcac aaagtaaagc aggaaaagca gtagatttaa aagatagaag caagtggaaa
     7981 gcaggagact tattatgtag aattggtgga ggaagtagta atcatgttgt aatgtacatt
     8041 ggaaacaatc aaataattca ttcaccacaa acaggagatg tggtaaaaat agagtctgtt
     8101 aattcttata gaaaaggaaa agcatacaca catgtgagaa gatttatata agtgaggtgg
     8161 caatatgagc caagatttat tacagataat aaaaaaagct gcaatggatg cagtagaaac
     8221 aagcaaccca atgagggttg tatttggaac aatagaaagt attagtcctc taagagttaa
     8281 gatagaacaa aaactatcta ttggtgaaat ttttctaata caaacagata catttaaaag
     8341 atatacagat aaaaaaatag gagataaatt agtcttaatt cgtatgcaag gagggcaaca
     8401 atacttgatc ttagatagga tgtgataaag tgttaccaag tgataattta gattatgata
     8461 ttgaagatgt atcaataatt aattttgatg taagacaaga gccaagtaag acatttaaac
     8521 ttcatataga aaagtctaag attgatggta tttgtgatga tgtagaagca ttaaaacaga
     8581 ccatcttttt gattttaaac acagagagat accaacatct aatatatagt tggaattatg
     8641 gagtcgagtt gaacgacctt attggagagc ctatatcctt tgtaatacca gagttagaga
     8701 gaagaatcaa agaagcacta attcaagatg atagggttga aaatgtagat aattttgagt
     8761 ttgaaaatat aaagggtaaa gtacattgta agttcacagt ttacagtaaa tatggaaata
     8821 taaaagcaga gaaagtggtg agtgtataat tgtttgagtt aatgacattt gaaaatataa
     8881 ttaaaagaat gttagatagt gtaccagata cttttgataa aagggaaggt tctataatat
     8941 ataatgctct tgctcctgtt gctatagaac ttacagaaac atacattgcc atggatgaat
     9001 tactagacca aacttttgta gatactgcta gttattacta tttagagaag agatgtaaag
     9061 aaagaggaat cacaccactt gaagccacta atacaattgc aaaaggagtt tttaacatag
     9121 atattccact tgattcaaga ttcaacttag gagagtataa ttatattgca actgaaagga
     9181 ttagtgaagg tatatataag atgaaatgtg aaactgctgg acctattttt gagttggggc
     9241 aactaatacc tattgaatat atagatggtc ttgaaacagc agagttaaca gaaatactaa
     9301 taaatggaga agatgaagaa tctgaggaca gtttaagaca aagatattat gatagtttaa
     9361 attcccaatc ttttgggggt aatatacaaa actataagga tgaagttaat aaattgcctg
     9421 acgttggggg agttaaagta tatccagttt ggaacggtgg aggtactgtt aagttagtaa
     9481 taattaattc taacttcaaa gttccatcta gtgatttagt taatttagtt caagaagaaa
     9541 ttgacccaaa tatgaaagga gaaggtttag gattagcacc aataggtcat agagtcacag
     9601 ttgaaggagt tacaagtaca actataaata tatcagcaga aataacatac aagagtggtt
     9661 acacttggga gaatataaaa tcaattgcaa aagaagcagt tgatgactat ttaaatgagt
     9721 taaatatgag ttgggaagat gaagaaaact taatagtccg tatatctcag atagaaactc
     9781 gtttgttaag tatagatgga gtattagaca ttgcaaatac aatgataaat gaggttaaat
     9841 ctaatctaac aatagatagt aacagcatag tagtgagagg tgaggtagtt ggataaagag
     9901 attaatctaa taaattactt accacaaatt ctacaagata aagaagaata tataaaagta
     9961 tttaatgtag gaaataaaga aataaaaata ttacatgata aattaaagga cctatcaaat
    10021 gaccagtttt tagaggacct aactataagt ggtataaaaa gatgggaaaa gataatgtct
    10081 ataactccta aaagtaatga gagtttagaa gatagaaggt ttaggatttt tagtaaatat
    10141 ataagtaaat tacctta
//
LOCUS       DAKBNN010000140        10119 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000140 DAKBNN010000000
VERSION     DAKBNN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.141"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            170..1618
                     /locus_tag="OUB02_002799"
     CDS             170..1618
                     /locus_tag="OUB02_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCT8886542.1"
                     /translation="MSILNLFRKNKNKNNVDCNEKSNESKQSYNDSTTLSLECNSNSP
                     HNIEVKNEINKVLNVSCNDTTNLYELEKQAREKSDALYDIHTNSINKFNPQNNILETD
                     TKPLTSIEKSFLKYIIGENIYEPYIATYWTYEYNINYSYLISKFFNMDYLKISNYIED
                     LTKLTVSELKEILKSNNIKSTGKKAELIERIEKEISCKDLSNFFNSSNKYYALTDKGK
                     ELLKDVRKSVTKNTDLEDQCLELIYIDKYEEAYDLICKYESSKNIQRGININWENHKI
                     TPMKIESYKAIKELDINLKDTLLDNIIKSSYILCDMLGNNSKTSILVKRLAGEKIDNI
                     EINNAINSINDVIYNHSTITYNANDSINNKNLHYMDIVKNNKSIPFEVNNKNYNFKIN
                     ENEIYFFNILKIKMYENELKNNFVFDRMSDGAFNVFDTSDNFIGKVKLQGRKKWIMYM
                     KNEFGSEHIYGELIHLIDGIDAWIKYSKEYLK"
     gene            1889..3553
                     /locus_tag="OUB02_002800"
     CDS             1889..3553
                     /locus_tag="OUB02_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCT8886543.1"
                     /translation="MNNENEQLKSDLLINDEKIKNLNFKIPWYYSLWTISILILSTFS
                     TYSISFIVAIIFLFKRNKIMKKHKDSISILLSDVEKINNKYILLNDEIKMKEKHFEDL
                     CESNENKLKELSNLLDKKKVEIDKFDSENQDKFKLIEELKIEKERLDNLIKDKNILKD
                     NINTLNAHLEELKDEREELRDINTTLKNKKEELKRLSEELIQTEDEVLLQSFGLYNPK
                     YDFENSDEYMEKLKEIRERQKLLIRNKTGVKYSDSWTVDGSIQKGRTMTNQNIKTALK
                     LFNSECDIAMSKVSFKNIDSIEKRIRKAFTDTNKLNTSNKVSIKENYLNLKIDELYLY
                     YEYLQMKEEEKEEQRALREQMKEEALVQKEIENQKRKLKKEELQFKNELLRLKSTIPE
                     DENDKLEWEQKINSIEEKLALLSKDLDDVLNREQNTRAGHVYIISNIGSFGENIYKIG
                     VTRRLDPTERINELSSASVPFKYDIHATIFSEDAPKLESALHKAFDNKRVNKVNNRKE
                     FFKVTLDEIRTEVEKNFDKTVEYTKLAEAQEYRQTLKIQELNKKLA"
     gene            4061..5179
                     /locus_tag="OUB02_002801"
     CDS             4061..5179
                     /locus_tag="OUB02_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT8886544.1"
                     /translation="MNIKSAFIRKRGEKFHVYVEYIEEMTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINSNKFITPSKTTLVERCYKYIMSNEKNWSPYTVINRKSWVKNYIEPFF
                     KDTKLIDINPVLIQTFINTTFNNCTFSSSKIRYRFISSVLKEAYRLREINENPCDFVK
                     LPHKENSSKIEIYNREEALLLIEKLNNSNIEIPILLMLTLGLRLGEATGLRWTDVDLN
                     IGSVSVSQTLIYANNKIEFKEPKTSKSRRTLSAPDELIEKLKIEKLRQNKLKLQGILK
                     NENNLVCLNKDYNPFLPATLRKSFHRFIKKNNLKNIRMHDLRHTNASLLLLGGTNMKV
                     VSERLGHTDIQITMNRYSHVLEEMDKKASDNLSKILFK"
     gene            complement(5333..5971)
                     /locus_tag="OUB02_002802"
                     /pseudo
     CDS             complement(5333..5971)
                     /locus_tag="OUB02_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(6112..6978)
                     /locus_tag="OUB02_002803"
     CDS             complement(6112..6978)
                     /locus_tag="OUB02_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCT8886545.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(7312..8859)
                     /locus_tag="OUB02_002804"
     CDS             complement(7312..8859)
                     /locus_tag="OUB02_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCT8886546.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(8852..9988)
                     /locus_tag="OUB02_002805"
     CDS             complement(8852..9988)
                     /locus_tag="OUB02_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886547.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLAVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
BASE COUNT         3943 a         1449 c         1219 g         3508 t
ORIGIN      
        1 ttttactttt tataccatat aaatatagta ttcttttcaa aactatttaa ttctatgttt
       61 tttcctaata tacatagcat ttatacagaa agttttatat acttaaattg ttattgtcac
      121 aaagtagaat acactagaaa ttaattaaat acaaaaggag taatttttta tgagcatttt
      181 gaatctattt agaaaaaata aaaacaaaaa taatgttgat tgcaatgaaa aatcgaacga
      241 atcaaaacaa agctataatg atagtactac actttcacta gaatgtaatt ctaattcacc
      301 tcataacata gaagtaaaaa atgaaattaa taaagtgctg aatgtaagtt gtaatgatac
      361 aacaaattta tatgaattag aaaaacaagc aagagaaaaa tcagatgctt tatatgatat
      421 acatactaat agtattaata agtttaaccc acaaaacaat atattagaaa cagatactaa
      481 accacttaca tcaatagaaa aatctttcct aaaatacata attggagaaa atatatacga
      541 gccttatata gcaacctatt ggacatatga atataatatt aactattcat atttaatttc
      601 taagtttttc aacatggatt atcttaagat ttcaaattac attgaagacc ttactaagtt
      661 aacagtttcg gaattgaaag aaatattaaa atctaataat attaaatcaa ctggtaaaaa
      721 ggctgaatta atagaaagga tagaaaaaga aatctcatgc aaagatttat ctaatttttt
      781 taatagctcc aacaaatatt atgctttaac agataaagga aaagaactgt tgaaagatgt
      841 gcgtaaatct gttactaaaa atactgacct agaagaccag tgtttagaat taatttatat
      901 tgataaatat gaggaagcat atgacttaat ttgtaaatat gaatcctcta aaaatatcca
      961 aagaggaatt aatataaact gggaaaatca taaaattaca cctatgaaaa ttgagtctta
     1021 caaagccata aaggaattag atataaattt aaaagacaca cttttagata acataattaa
     1081 atcatcctat atactttgtg atatgttagg aaataactcc aaaacttcta tattagtaaa
     1141 acgtttggca ggagaaaaaa ttgataatat cgaaattaat aatgctataa atagtataaa
     1201 tgatgtaata tacaatcact ctactataac atataacgca aatgatagta ttaataataa
     1261 aaatttacat tatatggata ttgttaaaaa taacaaatca ataccattcg aagttaataa
     1321 taaaaattac aacttcaaaa ttaatgaaaa tgaaatctac ttttttaata tacttaaaat
     1381 taaaatgtac gaaaatgaat taaaaaataa ttttgtcttt gatagaatgt cagatggagc
     1441 atttaatgta tttgatactt cagacaactt cattggtaaa gttaaattgc aaggtagaaa
     1501 aaaatggata atgtacatga aaaatgaatt tggttcagaa catatttatg gcgaattaat
     1561 tcacttaatc gatggtattg atgcttggat taaatattca aaggagtatc taaaataata
     1621 aaagagctac ttaaaatgat taaaactaat tttaagtggc ttttccttat gctcaaaata
     1681 ctgataacat acttttataa gaatgttata ttatttttct aatttctgtt aacatacctt
     1741 tataatccaa ctaaattctt ttaaataact accattttgt tttattttta cctttttttg
     1801 caaatatatg gaaattgcac ataaaatttt atatacttaa attattatta tcataaagta
     1861 gaacaaaaaa taaaaaagga gttttgatat gaacaatgaa aatgaacagt taaaaagtga
     1921 tttactcatc aacgatgaaa aaatcaaaaa tcttaatttc aagataccct ggtattattc
     1981 tttatggact atatccatac taattctttc tacgttttca acatactcta tatcatttat
     2041 agttgcaata atattcttat ttaaaagaaa taagataatg aaaaaacata aagatagcat
     2101 tagtatacta ttatctgatg tcgaaaaaat caataataaa tatattttat taaatgatga
     2161 aattaaaatg aaagaaaaac actttgaaga tttatgtgaa tcaaatgaaa ataagttaaa
     2221 agaattatcc aatctattag acaaaaagaa agttgaaatt gataaatttg attcagaaaa
     2281 ccaagataaa tttaaactta tcgaagaact aaaaatagaa aaagaaagac ttgataatct
     2341 tataaaggat aaaaatatac ttaaggataa tattaatact ttaaacgctc atttagaaga
     2401 attaaaagac gagagggaag aacttagaga tataaataca actttaaaaa ataaaaaaga
     2461 agagttaaaa aggctatctg aggaattaat acagactgaa gatgaggttc tacttcaatc
     2521 atttggatta tataatccaa aatatgattt tgaaaattcc gatgagtata tggaaaaatt
     2581 aaaagaaata agagaaagac aaaaactatt aataagaaat aaaacaggtg taaaatattc
     2641 tgattcttgg actgttgatg gaagtattca aaaaggtaga actatgacta atcaaaatat
     2701 taaaacagct cttaagctct tcaatagtga gtgtgacatt gctatgtcaa aagttagttt
     2761 taaaaatatt gattctatag aaaaaagaat acgaaaagca tttactgata caaataaatt
     2821 aaatacttca aataaagttt ctataaaaga aaactatctt aatttgaaaa tagatgagct
     2881 ttatttatat tatgaatacc ttcaaatgaa agaagaagaa aaagaagaac aaagagcttt
     2941 gagagaacaa atgaaagaag aagctcttgt tcaaaaagaa atagaaaatc aaaaaagaaa
     3001 actaaaaaaa gaagaattac aatttaagaa tgaattactt agactaaaat caactatacc
     3061 agaagatgaa aacgacaaat tagaatggga acaaaaaatc aattctatag aagaaaaact
     3121 agctttacta tcaaaagatt tagatgatgt attaaataga gaacaaaata caagagcagg
     3181 acatgtatac ataatctcca atattggtag ttttggagaa aatatatata aaataggagt
     3241 aactagaaga ttagacccaa ctgagagaat aaatgaatta agtagtgcat cagttccttt
     3301 taaatatgat atacatgcga ctatatttag tgaagatgca cctaaattag aatcagcttt
     3361 acataaagct tttgataata agagagttaa caaggtaaat aatagaaaag aattcttcaa
     3421 agttacgctt gatgaaataa gaacagaagt tgagaaaaat ttcgataaga ctgtggaata
     3481 tacaaaattg gcagaagcac aagaatatag acaaacgtta aaaatacaag aattaaataa
     3541 gaaattagct taaatatatt caaacaatta taatttaata aaatattatt tttgaatatc
     3601 tgtacaaatt tgtgatataa taatagcaag gagataaaat ttactttgta aaaggtgaat
     3661 ttctctaatt gattagaatt taaaagttat ttctttaaat cacccttatt ggcgtttggg
     3721 tgatttttta ttttctcgta aatgtaagct gacacaattc cagctactat actcaataaa
     3781 aagttaatta acatataaat tcacctcctt cctatattgg aatttggcgt ttatatgaga
     3841 aaatcaccct tagcttttca attttatttt ccttgctaca attattatat catataattc
     3901 ttacatattt tacctatata ttactttttt attcatccat atatctctgt atacatctat
     3961 ctattatcga atagtattta taacttagat ttgatataat aaaacaagag ctattcaata
     4021 tattttaaac atgaataata cttataaagg gagtgtacat atgaatatca aatcagcttt
     4081 tataagaaaa agaggggaaa aatttcatgt atatgtggag tacatagaag aaatgactgg
     4141 caagataaaa cagaaaagtt atgggagcta tgaaaagaaa aaggatgctg aaaaacattt
     4201 aattgaaata aaatctacta taaatagtaa taagtttatt actccaagta aaaccactct
     4261 tgtagagagg tgttataaat acataatgtc aaatgaaaaa aattggtctc cttatacagt
     4321 tataaatagg aaatcttggg ttaagaatta tatagaacct ttttttaaag atacaaaact
     4381 tatagatata aatcctgttt taattcaaac ttttataaat acaactttta ataattgtac
     4441 ttttagtagc tcaaagataa ggtatcgttt tataagttct gttctgaaag aagcatatag
     4501 attaagagaa ataaatgaaa atccatgtga ttttgtaaaa cttcctcata aagaaaattc
     4561 ttctaagatt gaaatatata atagagaaga agctttattg ttgatagaaa aactaaataa
     4621 tagtaacata gaaataccta ttttactaat gttgacatta ggtttgcgac taggagaagc
     4681 tactggactt agatggactg atgttgattt aaatatcggc tctgtaagtg taagtcaaac
     4741 acttatatac gctaataata aaatagaatt taaagaacct aaaacatcaa aatcaagaag
     4801 aacattgtca gctcctgatg aactaataga aaaacttaag attgaaaaat taagacaaaa
     4861 taaattaaaa ttacaaggaa tacttaaaaa tgaaaataat ttagtttgtt taaacaaaga
     4921 ttataatcca tttttaccag ctacattaag aaagtctttt catagattta ttaaaaaaaa
     4981 taacttaaaa aacattagga tgcatgattt aagacataca aatgcaagtt tgcttttatt
     5041 aggaggtact aacatgaaag ttgtttcaga aaggttaggt catacagata tacaaataac
     5101 tatgaacaga tactctcatg ttttagaaga aatggataaa aaagcatctg ataatttaag
     5161 taaaatatta ttcaaataaa tgattactgg ctgtcagtta aatgtcagtt gaacctaaaa
     5221 aaataggttt tgtcagttaa atgtcagtca gttaatttat aataatttct tttaaatatc
     5281 gctaaaacta cacgttttaa catttattta tacttattta taagtattta ttattatact
     5341 ttggaatagt tccaccactt aatggattca ttgccacaac accatatcca ttatcaacag
     5401 catatttggc accttttagc ctatatggaa agtttaatat atttattccc atagttacac
     5461 cttcaaactt attttcactt agaatatcta taacctcatc tcctggttga tgagatgaaa
     5521 atactatatg gtctatcaat ccttcttctt tacatttcaa taatccttca tattgacctc
     5581 caggtctcat tgctagttcg taatgttcca tctttctaat attccaaaca tgataaaaat
     5641 ttattttaga tacgcctaat cgttctattg atttttttac agcctctata gctttttctg
     5701 atgtatcaca tgcttttggc ttttctttag ttgatacata aaaatcggtc ttaccttcct
     5761 ttatcatttg tctaaaggct agtccaaata tatcttcact tctgtcatca caataccctg
     5821 gtgctgtatc aaagtaatta ataccttttt catacgcata tttgactaaa tcagcatttt
     5881 cttgattact tttttctaaa tcaaacctta atcctccata gccaactact gaaaccattt
     5941 tccctgtctt tccatattca acgtatctca ttgtaaattc ctccttagat atattaagac
     6001 ttaatttctt tatagaaaat attataccat aaataatttt tataaattat ttatggtata
     6061 atattttttt tataaattat atttttttat aacttgtatt ttataactaa gttaaaaact
     6121 ataaaaaggt tcaatatgga ctattacttg agcattttca ttaatttctt cttgtatttg
     6181 tttctcaatt ttatgtgtta aatcatgaga ttgctctacg cttataaatg ggtctaccat
     6241 tacatgcatg tcaatataaa tagaactttt gctacctcta cttctgatat tgtgaacatc
     6301 ccttacctca ttaaattcaa acacaatttc ttttatataa tcctcatcta cagccactct
     6361 atcgaccaaa attccaatag atggcctaaa tattccataa gccgaataaa ttatgaatgc
     6421 agaaataata ataggaacta tagattctat tattactgga aatcctaact taacaccaac
     6481 taaagtaacc aatacaccta gagacacaaa tatatcactt cttgtatgca atgaatccga
     6541 aatcaaaaca taactattta gtttagtccc aattctatat tcatacatgc atacaaaaat
     6601 atttataaaa agtgtaataa ttaaagcaat taaactttct atagttatag caggtacaac
     6661 tggattagct attcttaaca ctgcactaag aattatctta attgctataa caaacaacat
     6721 accacttata aataaactgg ttataatttc aaacttttta tgtccataag gatgattttt
     6781 atcttttggt ttagatgcaa agaaaatacc tataagcccc actatattag atgctccatc
     6841 tgacaaagaa tgaaatccgt ctgctgtcat actatagctt tttatttgat taccaataat
     6901 gatttttaat aatgctactg caaaattagc aaataaaatt atccacaata cttgcttcac
     6961 tttcttatag ttatccataa caaattcccc ctcccttcga ttcaataaag aaagctatct
     7021 atcaatatca atcaaccaga caataaaatc gtatataagc acatttacta tatttatatt
     7081 caaaattggt tgtacttgtt cattctattt gaaacttaat tataaataaa aatcgtaggt
     7141 atggttttgg tatacataaa aataagagac tgtctcaaaa taaagataat cttctttttt
     7201 attgttgaaa tgataaaata tatttgattt catataaaaa agagtgcctc atgaactaaa
     7261 aagttcattt tgagacactc ccttaaattt ttgatataaa ctattaattt tttatactaa
     7321 gccttcttta actaagaatg aatgagcaac ttcttctggt gatttaccaa gttcatccac
     7381 ttgataatta agttctctca tagtttcttc atttattttt cctgaaagca tattcataac
     7441 tttttctaac tcaggatatt tcttcaaagt atcttctctt actaatggtg ctgcataata
     7501 tggtacaaag aaatttttat cgtcctctaa tatcttaaga ttaaactttt taataagacc
     7561 atcagttaaa aatgcatcta taacctgaga ttcattatta tctaaagctg tatatcttaa
     7621 acttccattc attgctttta catctttgaa ttttaaacca taatctctag aaagccctac
     7681 aagaccatct tctctatttt caaactccaa agttggtgag aatgtaaatt ctttaccata
     7741 tttagtcaaa tcagaaatag tatttattcc atacttttca gcagtttcag gcatcatggc
     7801 taatgtgtaa gtattattaa atccaagagg gtcaagtaaa gttaactgat tttctttttc
     7861 catagtatct tttacaacat tatatgcttc atcagcatct ttaatcggag catgtctcat
     7921 tacatttacc aaaagtgtac ctgtatagtc aacatacatg tctaactcac cagatttaat
     7981 tgcattaaat gcaacagatg aaccacctag atttaaattc ttcttaactt taagattcgt
     8041 atttttttct actaattcag cataaacatt gccaagtata agttgttctg tataatcttt
     8101 agaaccaata gttattgtat cttttttact tgaaaaacta gaactaaaaa caacgaacaa
     8161 cattgaaaca acaaccacta cagatattac tttaagtgct atataattct tcttaggtgc
     8221 ttttggattt aatccttttg gagtaactgc aacttcaatt ttactaaata aataatcaac
     8281 taacagtgca attatacatg atgggattgc ccctgctaaa atcatattat tatttacagt
     8341 ttgaacccct gaaaatacta aatatcctaa accaccagca cctataaatg ctgcaagagt
     8401 catcaaacca acagctgtaa ctgcagatat tctaactcca cccattatta taggaagtgc
     8461 taaagggaat tgtattttaa ataatgtctg atttcttgta agaccaatac ctttagctga
     8521 ttctaatact actgggtcaa tacttgttat acctatatac gtatttttta caattggtag
     8581 taatgaataa accactacca taaatatggc tggtttactt cctataccaa gtataggtac
     8641 aagaagaccc aaaagtgcca tagacggaac tgcttggact aagtttacaa atccaagtac
     8701 aaactttcta agagatgaga ttcttgaaat aattattcca agaggaacac caactattat
     8761 agctattaaa atagatgtaa cagttagact catatgttgt attaaaagct ctataatctt
     8821 atctttttgt aatagtataa agttaaaaaa attagccaag aacactcacc tccatttcta
     8881 agaattgttc acttaaaaca gaaagtaaac tacttctagt aataagccct accagtttat
     8941 tttcatcaga aataaccgga atatatccaa ccgaattacg attcattaca tttagtattt
     9001 ctactaaatt atcaccttca tttacatgca aaggctcact tgacataata tcagcaagta
     9061 atattgactt atcatcaata tctttcatat cttttacagt aacaatacct tttaatgttt
     9121 ttgctctatc tataattagt aagctatcaa ccttactcgt tctcataatt tctattcctt
     9181 gagttacagt tctagtagaa ttaacagcaa taggattttt tatcataata tctcttgctt
     9241 ttatatattc tggattatcc caaactctat cttctccgat aaaatctttt acaaagtcat
     9301 ttgcaggctt tctaagtata ttttcgggag tatcatattg tgctattctt ccatccttca
     9361 taatacaaat tttatcagct atctttaaag cctcatccat atcatgcgtt acaaatatta
     9421 tagttttttt caattcatct tgaagagaaa ataattgttc ttgtaaagat gttctcgtta
     9481 ttgggtctaa agcactaaaa ggctcatcca tcaatattat ttcagcatca gtagcaatgg
     9541 ctcttgcaac accgattctt tgttgctgtc caccactaag ttctgaagga tacttattca
     9601 aaaattcatc actatcaagt cctaccatct caagtagttg caacgttctt ttttcaattt
     9661 cttcaacgga tttttccttt tttaggcgtg gaattagctc tatattttct tttattgtaa
     9721 gatgagggaa taggcctgtg ttctgtataa catatcctat ttttcttcta agctctattt
     9781 cattttcctg agaaattggt ttgccattaa tatatatctc accagatgta ggttttatta
     9841 atttgtttat aagcttcaaa gtagtagtct ttccacaacc acttgaacca atcaaaacta
     9901 ccaatgtacc tgtttctaca gctagagaaa tatcatctaa tataacatta tttcctattt
     9961 tttttgttac atttcttatc tctatcataa tttatcatcc cctttttcga acaagcaacc
    10021 ttcttcaata ttataagttg tttttataat tatatcatac tattttattt atgtaaacaa
    10081 agtacgattt ttaggtcact atttgtctga atattgcta
//
LOCUS       DAKBNN010000141        10116 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000141 DAKBNN010000000
VERSION     DAKBNN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.142"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            469..1311
                     /locus_tag="OUB02_002806"
     CDS             469..1311
                     /locus_tag="OUB02_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8886548.1"
                     /translation="MIINKILNNNVIITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            1456..3384
                     /locus_tag="OUB02_002807"
     CDS             1456..3384
                     /locus_tag="OUB02_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8886549.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISVGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            3445..4872
                     /gene="ascB"
                     /locus_tag="OUB02_002808"
     CDS             3445..4872
                     /gene="ascB"
                     /locus_tag="OUB02_002808"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCT8886550.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFISA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            5283..6059
                     /locus_tag="OUB02_002809"
     CDS             5283..6059
                     /locus_tag="OUB02_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8886551.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAIALTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            6075..6440
                     /locus_tag="OUB02_002810"
     CDS             6075..6440
                     /locus_tag="OUB02_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT8886552.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(6607..7356)
                     /locus_tag="OUB02_002811"
     CDS             complement(6607..7356)
                     /locus_tag="OUB02_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HCT8886553.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            7554..7706
                     /locus_tag="OUB02_002812"
     CDS             7554..7706
                     /locus_tag="OUB02_002812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886554.1"
                     /translation="MRRDNNLLMKDYKYKGYFKGVEIFDNVNDLNLNTLTTVSRFVLV
                     FLFTIT"
     gene            7834..8259
                     /locus_tag="OUB02_002813"
     CDS             7834..8259
                     /locus_tag="OUB02_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886555.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            8291..9478
                     /locus_tag="OUB02_002814"
     CDS             8291..9478
                     /locus_tag="OUB02_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCT8886556.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTNIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGT
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            9670..10074
                     /locus_tag="OUB02_002815"
     CDS             9670..10074
                     /locus_tag="OUB02_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCT8886557.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKGDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         3922 a         1066 c         1767 g         3361 t
ORIGIN      
        1 gaggttaata attgaattta taaatcgaaa aacaacctat tgtgagagct taaattacta
       61 tagaaggtcg ttttttatta cagaaataaa taagagatag agaatagttt agaggtgaga
      121 aagaaaatat aatttaaaac atataacaac atataatctt ctaatagata aaggatttaa
      181 tgttgttgat tttaaaaatt ataataaaac tattaaaaat ggactctatt aaattaatat
      241 taataagaat aattattata actgacaaaa agcaacacaa aagattgatg aagtatatgg
      301 aaataaaaag ttgacaaaat atgtctgatg ttttatcata ttaattggaa ttaggtagta
      361 attgtttaag gacaagctaa gcttattcat taataatagc agtatcctag gaaggagtat
      421 gcccattatt atggataagc tttttttatg cttaaaagga ggataatcat gataattaat
      481 aagatattaa acaataatgt tataatcaca ttagatgata atgatgaaga agttatcgtt
      541 atgggtaaag gaattggtta tcaaaaatca aaaggcaatc taatagataa aactaaagta
      601 aataaagtat ttagaatatc aaataaagaa atatcaaaca aactacaaga attactcaat
      661 aatattccta tagaacacat gaaattatca agtgaaatta tagaatacgc acaaataaag
      721 ttaaacaaga agctaaatga aagtatttac atatccttaa gtgaccatac atattctgca
      781 attcaaagaa tgaaagaagg aataaatgta aaaaatgcaa tcctttggga aattaaaaga
      841 ttttataaag aagagtttga aattggaatg aaagcactag atattattga aaacaaaaca
      901 ggtataaaat taccagaaga tgaggctgga tttatagcct ttcatatagt gaatgctcag
      961 ttaagtgaag gtcatacttt ggcatctgat attacgaagt taatacaaga agtacttagc
     1021 atagttaggt atcattttag gatagaattt catgaagaat cagtgtttta ttatagattt
     1081 ataatgcatt taaagttttt tgcgcaaaga ttgcttttag atagtgcaca tgaaggtgag
     1141 acagacaaag aattactaag cattattaaa tctaaatata ataaagagtt tgaatgtgta
     1201 gttaaaatta aaaatttcat taaaaaacaa tataactaca tattaacaga tgatgaactt
     1261 atatatttaa caatacattt agctaaagta gttaaagact caaatatgta agtgaaataa
     1321 tttttcagca gtatattaag aatgattgaa aggagtgata ctaacaaaat tatatttatg
     1381 gttggatggt aacattgagt tggccaaacc ttcaaatttc aaaaaattaa tttaaacgaa
     1441 atatggagga gaaaaatggg aaaatacaaa caattagcac aagagatagt aaacaatgtt
     1501 ggtggaaaag aaaatattag tggattagta cactgtatta caagattacg ttttacttta
     1561 aaagatgaaa gtatagcaaa tgacaatgta ttaaaaaaca tggaaggtat tgttactgta
     1621 atgaaaagtg gaggacaata tcaagtagtt ataggaaatc atgttgaagc tgtctataaa
     1681 gatgtagttg aaataattgg tttagatgat tcaagtacaa gttcagaaac taaaaaatca
     1741 ggtaatatac tagataaagg tatagatata atatcaggaa tttttcagcc agtgttagga
     1801 ataatggcag cttgtggtat gttgaaagga tttaatgcat tatttgtagc aatgggatta
     1861 tatagtgatg tatctggtgg ttatatggta ataaatgcag cgggagatgc attatttaca
     1921 ttccttcctt tgttcttagg atatacttca gctaagaagt ttggattaaa accaatgttg
     1981 ggattagcac taggagctgc agtatgttat cctgctatac aaggtagcag tatatcagta
     2041 ggagcagatg tgatgtacac tttatttaaa ggaacaatgt ttgcatcacc agtatatatt
     2101 gatttttttg gactaccaat tgtatcaatt gattatactg gaacagtcgt accaattata
     2161 tttatagtgt attttgcttc taggtgtgaa aaattattta ataagtgtgt tccagattta
     2221 gttaagtttt tctttgtgcc aatgcttaca ttattaataa cagtgcctgt tgcacttata
     2281 gttataggtc ctatagcaac atttggttct acacttattt cacaaattgt aatttcaata
     2341 agagatttta gtccactatt ggcaggtgct atagtaggat ttacttggca aattcttgtt
     2401 atttttggta tgcactgggg atttattcct gtatatataa acaatgttat gactttagga
     2461 tatgataatg ttatgatgcc attctttgcc tgtacatttg caagttcggc agttgtgttg
     2521 gcaatatttt tcaagactaa agataaaaaa ctaaaagaga tggcaatacc aaactttata
     2581 tctggtatat ttggagttac agagccagca atttatggaa tattattgcc tttaaagaaa
     2641 ccatttataa taagttgtat tgctagtggt attggaggcg ctttctatgg attctttaat
     2701 cttagaaaat ttataactgg aggaatggga atatttgaac ttccagcaat gattgaacca
     2761 gatgggggaa tgggtaactt aatagtagct ctttctggta tagctatatc aatggtcgta
     2821 gcttttgtat taactatgat tttatataaa gatgagaaag tagaagagtc taaaaaagta
     2881 agaaataagg gtaaagaaga aataaaagaa gtcaagtcta ctaaattaga aagagaaata
     2941 gtaacaagtc ctataaaagg tgaagtatta aaattaagtg atatagagga tgcagcattt
     3001 gcatctggtg tactaggtca aggggtggca ataataccta gtgatggaaa agtagttgct
     3061 ccagtagatg gggtagttac aaccttattt cctagtctac atgctatagg gattctttct
     3121 gatacaggtg ttgaagtgct tattcatatt ggattaaata ctattcaatt agagggaaga
     3181 ggttttgaag catgtataaa acaaggtgat agaattacaa aaggtcaaac tatattaaac
     3241 tttgatgttg atgcaataaa agagttgggg tatagtactg taactccaat tgttattaca
     3301 aattcatcac aatttttaga tgtagtagaa acagaaagta aaaatattga attagaagac
     3361 aatctaatta cagtcttatt ttagtaaaga ttgaggtatt agcataatct agtgctttgg
     3421 ttataatttt aggaggcttt gactatgagt tttaagaatg attttttatg gggaggagct
     3481 actgcggcca atcaatgtga aggtggatac aatgaagacg aaagaggatt ggcaaatgta
     3541 gatgtatgtc caacaggaaa agatagaaca gcagttatta cagggaaact aaaaatgttt
     3601 gattttgatg atgagcatta ttatccagct aaaactggta ttgatatgta tcataattat
     3661 aaagaagata taaagttatt tgctgaaatg ggatttaaag tatatagaat gagcattgca
     3721 tggagtcgaa tatttccaaa aggtgatgaa gaaacaccaa atgaaaaggg acttcaattt
     3781 tatgaaaata tatttaagga gtgtaaaaaa tataaaatag agcctttagt cactattaca
     3841 cactttgatt gtccaatgca tttagttaaa aagtatggtg catggcgtaa tcgaaaaatg
     3901 ataggtttct atgagaaatt atgcaatgtt atctttagaa gatataaagg attagtgaag
     3961 tactggctaa ctttcaatga aataaatatg attttacatg ctccatttat gggtgctggt
     4021 atttgctttg aagaaggtga agatgttgaa aagataaaat atcaagctgc ccatcatgag
     4081 cttgtagcta gtgcaattgc aactaaaata gcacatgaag ttgattcaaa taacttaatt
     4141 ggatgtatgt ttgcagctgg aagtgtatat ccttatagtt gtaatcctaa tgatgtatgg
     4201 gaagctacaa agttagatag agaaaattac ttctttgtgg atgtacaatc taaaggtaaa
     4261 tatcctaact atgcgttaaa gtatatggag caaaaaggta ttacaccaga aatggaacct
     4321 ggtgatattg aattattgaa taaatataca gtagatttta tttcattttc atattacaat
     4381 agccgttgtg ttagaaccga tgaaaatgca gatgatatgg ctgaaggaaa catatttata
     4441 tctgctaaaa atccatatct ttcatacagt caatggggat ggccaattga cccattaggt
     4501 ttgagaatta cactaaatca tgtatatgat agatatgaaa agccactatt tattgtagaa
     4561 aatggattag gtgctaaaga tatagctgat gaaaatggtt atgtagaaga tgattacaga
     4621 attgattata taagagaaca tataaaagct atgcatgatg cagtttctat agatggggtc
     4681 gatttattag gttatactac atgggcacct attgatttag taagtgctgg tacaggtgaa
     4741 atggaaaagc gttatggatt tatatatgta gatagagaca atagtggtaa tggaagtcta
     4801 agaagaatga aaaagaaaag ttttgaatgg tataagaaag taattgcttc aaatggtgaa
     4861 gatttatatt agacaagaaa tctatgttaa ataagaaatt tgtattaaat aagaagagta
     4921 taaaaattaa taataagtat aatttttaac ttttttaact atagaaaatc aatatagaat
     4981 tattttacat tgtattgatg caatagtatg attcacaaat aaaattttag aaaataaaat
     5041 tgttaagaag tatgggaagt aagtgcaatt tactttgtaa taaaagatga aaaaggcaag
     5101 cttaggtttt aagcttgtct tttttattat gtataaacta tgatatttta aaattctatg
     5161 aaaaatttat ttaatagaga aattaaaaat ttaatggtaa tcaaattctt taccaatgaa
     5221 tataaaaaat gtctacgtgt tataatagtt taaactaata aacgaaaggt agatgaatga
     5281 atatgaaata ccaacatata atagaagttg acaaagaact ttgcattgga tgtgggcttt
     5341 gtaaaaatga ttgtccagta aacaatataa ttatagaaaa taaaaagtca gtaattaaaa
     5401 aacaagattg tctaatgtgt ggacattgtg cagcaatatg tcccacaaaa gctattgcct
     5461 tgacaggatt tgatgaacct cctatagaac ttacaaataa gccaaagcta gattcagatg
     5521 aattactaat ggcaattaaa tctagaagaa gtatacgtaa atttaaagat aaagaagttt
     5581 cttcagaaat tgtaaagcaa attattgagg caggtaggta tacccctagt gctaaaaatt
     5641 cacaagatgt atcatatatt gttttagaca ataaaaagtc aatttatgaa aacgaagcag
     5701 tgaaattttt tagaaaaata aaacctattg caaatatagc tattaaatat tcaaaagaag
     5761 ttgagattga cgataacttt ttctttaagc atgctcctat tgcaataatg ataattacaa
     5821 aagataagat tagtggttca cttgcagcat caaatatggc attaatggca gagtcttatg
     5881 gcctaggtgt tttatttagt ggatattttt cagatgtagc aaataattcg ccaaagttaa
     5941 aaaagctttt aggtctaaaa cgcagtaatc atgttcttac aacattagta attggatacc
     6001 cagatgttaa atatagacgt actgcacaga aagaagtagc aactgttaga tacttataag
     6061 agagggtgga ttttatggaa aaagaatgtc aacaaagaat gcaagaaata acgtctaaat
     6121 ttaaagaatg tcgcaaagct atctctgcta tgggtgatga gactagacaa ttgattttaa
     6181 ttgctctctt agagagtgat tttaatggta tacgagtggg agaaatcaca aaaaaaacac
     6241 atctttctcg tcctgctgta tctcatcatc ttaagatatt aaaagaagca gagattgtta
     6301 atttgcgtag agaaggaacg aaaaattatt attatcttga ttcaaaaaaa tcacaatgga
     6361 agtcactaac agagctaata aatcttatat ttgttggaat acaacatatt agtgaaaatg
     6421 atataagaaa aggtgattaa attatacttg atttaataca aaaaagattg atttaggaac
     6481 taaaaagttc actttgagac actatctttt gaactatacc catcttagga gacagttaaa
     6541 atatgtaaaa ctgtataaaa caatgggtat agttcatttt ataatggttt aaatattaag
     6601 aatatattat tctaaatagt atctttctct gttttgagtt ttatttttat actcaatagc
     6661 ttcagtgggg caaccacaaa tacatgccat acaatgagta cagttatttt tccaaactgg
     6721 ttttttgttt tttaaattaa tattatttaa aggacatagc tctacacatt ttccacagcc
     6781 aatgcattta tcagtataat gaaatccttt ggcactaata attgtcttat aaaaaactgt
     6841 atttattata ccacttttaa ttgtacctcc taagccacta ggagtaatag tttcaaattc
     6901 attttcattg cgtatatcag aaataatcct atatatttgt ttatctgctt ctttaatcat
     6961 ttgttttgat gtttctttgt ctgttgtaga aaataaagct atataattct ctggcatttt
     7021 aatttctgcc atacctttta attgccattc cttatattta catagttttt gaatatagtt
     7081 tatggatttt gctgtatctc caccacaagt cattacaaag taaactctgt tgcttcctag
     7141 aaattttgtt tcttttataa aatccgtaac cactattggc aatctccatg cataagtagg
     7201 acatacaaat atgaatggtt tatctgctga tattaattca tctgtttttt cctcttttat
     7261 aagttgattt agtgatatca gttcatcatt taattcttgt gcaattttac gagccacata
     7321 tcggctatta ccagttccgc taaaatataa aatcataata atatctccct aactattttt
     7381 tattttaact cttaattttt tatttattat agtaatttat tgatatattc tattcaaatg
     7441 tatgtaaaat ttagtagtta agaaaattat aacacattag taatttttat acttattggt
     7501 aaagaatttg attaccatta aatttagcaa caagacatca actggacttg tatatgagaa
     7561 gggataataa tttgcttatg aaagattata aatataaagg ttattttaaa ggtgtagaaa
     7621 tatttgataa tgtaaatgac cttaacttaa atactttaac aacagttagt agatttgtgc
     7681 ttgttttttt gtttacgata acttaaggaa atatgttacc tgaataataa atttatataa
     7741 taaaagtata taaatatata tatgaatgtt aagattagtt gatagaattt aaataataaa
     7801 tccaatatta taaacatagg aggtatgaaa aatatgatta atgagaattt aaaaagtcta
     7861 agaaaaatca accagtatac acaagaagaa ttagctgaaa aacttaatgt ttctagacaa
     7921 tcaatagcaa aatgggaaag tggagaatct attcctgata taggtagttg cattaaactt
     7981 gcaaaattgt acaatgtcaa actagatgac ttagttaatc attcagaaga aaaaacaggt
     8041 attatagtac ctcctaaagg aaagtttttc tttggagcag ttgtagtcgg tgaacgtggt
     8101 cagattgtca ttccaaaaga agcaagagaa gtatttaata taaatgcagg agataaatta
     8161 ttggtacttg gagatgagga aagaggatta ggaattgttc atcaacgtga tttgataaat
     8221 tttataggtc atgtgggagt agtacgtaaa gaagaatgat ttaaaaaaat tataaaagga
     8281 ggtatgaaat atgagtaaaa tagtattttt tagcattcct gcatatggtc atactaatcc
     8341 tactattgaa gtagttcgtg aattagtgga tagaggaaat gaggtattgt attattcttt
     8401 taatgaattt aaagataaaa ttgaaggtgc tggagcaaaa tttatttgtt gtgataaata
     8461 ccttccagaa ttactccctg gagatgagaa aaaaattggt aaggattttc ctggattaat
     8521 tgagatgata gtagatacta ctatttcttt agatgaaaaa gtttgtagag aacttaaaga
     8581 ttttaatcca gattgcattg tatcagattc attaagtttt tggggaaagt tatttgcaca
     8641 aaaacttaat attccatatg tatgctctac aacaactttt gcatttaata aacatacagc
     8701 aaagttgatg aaacaaaact ttatagaaat tatacgtatg atttttggtg taagacgcat
     8761 taataaaaaa ataaagcttt tacaaagaaa agggtatgaa gtaaaaaatt ttatttctat
     8821 agtttctaat gataatgata cggataccat tgtatataca tcaaaagaat ttcaaccaat
     8881 ggtagaaacc ttctctagta agtattcatt tgtaggtcca tcagtatcaa agttgattat
     8941 tgaacctaaa gagagaaaaa gaaagctaat atatatttcg cttggtacaa ttaataataa
     9001 aaatgttagc ttttataaaa actgtataaa tgcatttaaa gattctgatg ttgatgtgat
     9061 tatgtctgtt ggtagaagta ctaatattaa aagtttagga aatataccaa ataattttga
     9121 ggttaaaaat agtgttgaac agattgctat acttcaaaaa actgatgtgt ttgttactca
     9181 ttctgggatg aatagtgtta atgaaagttt atattatgga gtacctatgg tattatttcc
     9241 tcaacattcg gagcagagaa tggtagctaa gagagttgtt catttgggaa caggaattat
     9301 gttaaaagag gataagtcag aaagtattaa aaaagcagta tttcaagtta taaatgacaa
     9361 tgaatataaa gaaaatgcaa ctaaactatc aaaaagcttt tataatgcag gaggctcaaa
     9421 aaaagcagca gatgtaatat tacagattat atgtaatagt agacaagttg acatataata
     9481 gatttgttaa aaatatatat acatacatgt aatttaatca aagtactgac agtggggtct
     9541 tttaacctac cagttggggc atatatattg atgctaaata tgagggatgt tatattttaa
     9601 taaaaaagat aacatccttt ttttagtgca ttatatcgag ttttaaataa taaatgaagg
     9661 aggattaata tgaaaaaata taaagttatt tttatagtat taattatagg tattataagt
     9721 gtaacagggt ttgttgtata taaaaactac attaaaccat ggaaagatgc tgaaacatat
     9781 attgataagt atatggtaaa gcaagatatt tcaaaagata atataaaaag tattacaaaa
     9841 gaaaaagcca agaaggcaag ttacgaagga atactataca aagtgtatta caaaggtgac
     9901 cctcgatatg agtatcaata tttttatagt gataattatt atgctctata tgtaaataaa
     9961 gtaatgttac aaatttatga tatggaaaat aataataaac ttttaagaag taatgaggaa
    10021 ttaaaaagtg taaagtatcc accaatatat cttaaatata agccagataa atgatttatg
    10081 acaaaataga atgattttta ttaaaattta tttaaa
//
LOCUS       DAKBNN010000142        10011 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000142 DAKBNN010000000
VERSION     DAKBNN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.143"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..616)
                     /locus_tag="OUB02_002816"
     CDS             complement(<1..616)
                     /locus_tag="OUB02_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCT8886558.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKGFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMA"
     gene            complement(949..2163)
                     /gene="dpaL"
                     /locus_tag="OUB02_002817"
     CDS             complement(949..2163)
                     /gene="dpaL"
                     /locus_tag="OUB02_002817"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCT8886559.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKLLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2777..3316)
                     /locus_tag="OUB02_002818"
     CDS             complement(2777..3316)
                     /locus_tag="OUB02_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HCT8886560.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(3393..5198)
                     /locus_tag="OUB02_002819"
     CDS             complement(3393..5198)
                     /locus_tag="OUB02_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886561.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5594..5971)
                     /locus_tag="OUB02_002820"
     CDS             complement(5594..5971)
                     /locus_tag="OUB02_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCT8886562.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6876..7361
                     /locus_tag="OUB02_002821"
     CDS             6876..7361
                     /locus_tag="OUB02_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886563.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7561..8100)
                     /locus_tag="OUB02_002822"
     CDS             complement(7561..8100)
                     /locus_tag="OUB02_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8886564.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8093..8506)
                     /locus_tag="OUB02_002823"
     CDS             complement(8093..8506)
                     /locus_tag="OUB02_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8886565.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8542..9663)
                     /locus_tag="OUB02_002824"
     CDS             complement(8542..9663)
                     /locus_tag="OUB02_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCT8886566.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
BASE COUNT         3125 a         1820 c         1068 g         3998 t
ORIGIN      
        1 tagccatttt ataaactgca tttatacctt tttctggatt agcagagtga gctggtttac
       61 caaaagtttc tactactatt tctcctctac ctctttgacc tatctttagg tttaattctg
      121 aagattcacc tattacaaca taatcaggtt gaacagcctt acttatctct ctagctgaaa
      181 caccttcaaa tatttcttca tgaacaacac ctgctacata caattcacct tcaaagcctt
      241 tgttagtatc ttctgcaaaa tatgaaacag cagccatcat agcacttact tgccccttca
      301 tatcagaagt tcctctacca tatattttcc catctactat tctcgcttca aaaggaggaa
      361 cactccattt acttgcatct ggaactggaa ctgtatctat atgcccgtca aataatattt
      421 ttttacctgg tttatttcct tttatacatc ctactatatt tccatatcta tctcttgacc
      481 aactatcaaa acctagtaaa gcaaaatttt cttctatagc ttttactacg ttttcttctt
      541 gacctgaata actagcattt cttattaagt cttggcatac ttcagttaat tgtttttttc
      601 ttgtttcatt taacattttg atattccccc atataatttt tagtttgttg acgttttatt
      661 ttgatatttc acttttttaa tttattagca tttcacttaa tctattgatt ttttatttct
      721 ttatatttta tttattaaat ttttatttag actttatttt aattttattt ggttttattt
      781 aattttattt agtttttatt ttgaattttt catttgcttt actttttcat ttatttagat
      841 ttttcatttg ttttagtttt tattttattt aaagttttca cttatttaag tttttatttt
      901 ccttaaattt ttcatttatt tgttaagttt ttcttttcct taagtctttt atttactttg
      961 gcattcacca ttccaaacta tatctctata tttatcaggg tcagtatctc cttctgtact
     1021 tattaataat actttagagt tctcatctag tttcaaagct tctcttaagt cttttaaatc
     1081 ttcactttca aggattgaaa ctaatgctcc agttgtaact gctccagatt ctccagagat
     1141 tactttctca tctcctctta gagggtttcc taatattctc atacctttag cagataccca
     1201 gtctggtgct gaaacgaatg ctgatgaata attttttaac acttcaaaac ctatagtgtt
     1261 tggttctcca caagcaagcc ctgccattat agttggcata tctcctccaa caaatcttgg
     1321 ttttccatct ccagcttctg ctgatttata gtaacaatca gcctcatcag cctctactac
     1381 tactgttatt gggcattcat catatattga agcaacatat ccttgaactg caccagcaag
     1441 tgaaccaact cctgcttgaa caaatacgtg tgttggtcta tctactccat attcttttaa
     1501 ttgttctata gcttctttag ccatagttcc atatccttgc attatccatg ctggtatttc
     1561 ttcatatccg tcccatgctg tatcttgaac cattactcca tggtcatcag cttctgcata
     1621 atcattagct aatcttactg catcatcata gtttaagtct gttatactag catctgcacc
     1681 ttctcctttg attttattta atctaaattc agaagaacct tttggcatta atacaacaga
     1741 tttttgacct aatttattag ctgtccaagc aactcctcta ccatgatttc catctgtagc
     1801 tgtaaagaaa gttatttctc ctaatttttc ttttatttct tttgaagtta atttatcata
     1861 ccctaactca gatatatctg tatttaatct ttgagctaag tatctaccca tagagtaaga
     1921 accacctaaa actttaaatg cgtttaagcc aaatctataa gattcatctt taacatatat
     1981 tccgcttacg cctagtaatt ttgctaagtt atctaattta actaaaggag tcttttcata
     2041 ttgtggaaag ctctcatgaa aagctttagc ctttgcaatc tcttcatctt ttaaaaattc
     2101 aattcctttt tctttttcct ctgatttagg taagttattt aatttccatt ttatttctct
     2161 catttatttt atattcccct ttcaaatttt tcggtgtcat tttaattttt tatctttgtg
     2221 ccttatagca aagcaattac cgtgccaata ttatttattc taaaaacaat ttttttatgt
     2281 tggaattaca tacttttata aaatttcttt aaagtttata aattttgagt gtgaaaaata
     2341 agatttataa aagaaaaaat tctcatattg ggaaattttt atcaatacga gaattatcaa
     2401 ttcgagaaat cgtttatttg tcttaattct tcctttatta tgctcttatt ttttatatgt
     2461 ctattgtttt gtcagatata ttactacttt tattttctat aaatatatgg ctataacctt
     2521 cttacgcata aactctataa atttatatac tttatatact ttatattctt tattacccaa
     2581 agcctccaaa atggcttaaa tcttttcata gcttcttaca gaagtttaca tgggcgtccc
     2641 cccaataagc aaaagtgcaa tatgtgtacc attgtgccct acttgcgact attgagctgt
     2701 cgaaattcgc aacttcgcat ttatctgatg aaatgcacta tacaaaatat tcataacatc
     2761 cacaacagag ttaaagttac ctattcatat tcactgtaaa aatttcttcc acaggaaatg
     2821 taattttaac caatcctatt gagacaaact caccacttat ttttttgaac tcacgcagta
     2881 atacatcact tgcccgaaaa aaagcctcac tttcatcatc tccagtaagt gctattgtgc
     2941 aatggggtac ccaacgattg gggtggtacc attcccaacc atttgaatca aaatcttgta
     3001 gtacctcata taattctctc tgaaactgat acatactgct attcattata ggtgatacaa
     3061 acaccgtttt agtatcatta aacatgccta ctgaaccaat gtaagcaggt attaccttat
     3121 ggttttgtgc aaaccccttt agcttgtcta tgcaacaagc ctcatccaca tcattgaaac
     3181 aagccaaagt aagatgaggt cttgttttcc attctaagta cttcgtacta attttttcat
     3241 cagcgatttt tttagataaa tcaaaaagtt gcttttccgt ttttttatca taatatagct
     3301 ctatcgcata ttgcataata acacctccac cataaagtaa aaatacctat ctataaaaac
     3361 acacaaatac ctatcatttt aatattatat tactactgtt cctcttccaa ttttctatat
     3421 agtgtagcca aacctatgcc taagtctttt gctatcttct tcttcgtctt tgtatctctt
     3481 ccatacttat tcaacaactt gtttatgtaa gtcaactcta attctttaat tgaaagtatc
     3541 ctttcttgat tctctactat ccctccaact atatcatctt caaatattat atttacatcc
     3601 ttatttttat aaatatttcc attaatatta taatagttta aaatatctaa tggaagcata
     3661 tctttatata ttattccatc atctccaacc aaattcatca tatattcaac tgcattttcc
     3721 agttccctta tatttcccgg ccaattatag ttaatcaaca tattcattac attttcttct
     3781 attttattta actgaatacc taattctaaa gaatatttag aagcaaactt catcattatt
     3841 gcctcaatat catcctttct ttccctaaga ggtggcaaat ttataggaat tacattcaat
     3901 ctatagtata aatcatccct aaacttacct tcatttacta atttcaataa gtttttattt
     3961 gtagcagcaa tcactcttat atctaaatct ataagtttat ttgacccaat tctcgcaaat
     4021 tttctctctt gtaaaactct aagtaacttt gcttgtaaag gcattgacaa atctcctatt
     4081 tcatcaagaa atataacacc ttcatttgca agttcaaatt tacctacttt ccctccacta
     4141 ctagctccac taaatgcacc ttttatatag ccaaatagct cactttcaag gagattttct
     4201 ggaatagcag cacagtttat cgcaataaat ggcttgttcc atctgctacc ttcagcatgt
     4261 atagccctag caattagctc tttacccgta ccactttccc cagttataag tacagttgaa
     4321 tttgactttg ctaatttctt agttctctct tttactttta acattgcttc tgaatttcca
     4381 attatagact ctatatcact atttccccag ccactattca cttttccatg tcctttatga
     4441 ttgatatatg tcttgtcaaa tattattatt ttgtcgtatt gatttatata tggatatacc
     4501 ggaattattt tggcatttat attataaatt ttgttatcaa tatcaatttc tagtaattca
     4561 tgtccatttg aggaagattc aacagatact atatttacta tattttctat tatattttta
     4621 tctatatcta atttttttag agctatattg tttatataag atattttgct atttatgtct
     4681 agtattataa cgcctttttc aacattttca atgatttcta tcatcatatc catttttcta
     4741 ctgctatttt ttgacgcttc atgttcttct attttcatag aaattaaatc gcatatatga
     4801 ttaacaaatt ttaaataatt atctattttt gcacttattt ctactttttt agttttatct
     4861 gtactaatag caccaattac tcctatagtt cgattattat actttattgg aactgctatt
     4921 attactttat ttaagcattt catgtaatgg gaacattcta tacaaagctg attatcacat
     4981 atatccaata cgactaattc ctgacctgtc tgtataacat tgtcataaat aaatccttct
     5041 gtaacaacgc tttcatttat ctttgattta tacagacctg taccactaat tcttattaat
     5101 ctatcgtcaa ctatttcaat atctacatct aacacactag ctactgtttc agtgtatttt
     5161 atcatttctt gttgaaattc ttttaattta cttaacatag tttcccccat tagaatttaa
     5221 tttaccaaat tatcagttta gctatatttt aacatatagt cactctttaa aatatatata
     5281 aaacattact tatttatata tttgcgttaa aacagacaat attaatttat aaaaagtttg
     5341 ttttatatag taaccttaat tataatatgc agacaataaa ataaatcaca atacaataat
     5401 aaaaaaaagc tttcctcata taacaaatat atttttgaca taaaaatata cattatatga
     5461 gaaaagctca atagttaaat atttagatag atttttatat agactttttt aatataaact
     5521 tttttaattg tagacttttt ttattagttt ttaattttta ttagttttgt tattggtttt
     5581 attgttataa tacttataat actgccattg cttcaatttc taaaactgca tcttttggta
     5641 attgagcaac ttgtacgcat gaccttgctg gtggattttc tttaaaatat gtagcatata
     5701 cttcattaat atcattaaaa tcctttaaat cttttacgaa tactgtagtc ttaaatattt
     5761 tatccatact tgtaccagct tgttctaata ttgcttttac attatctaaa gaagcttttg
     5821 ttgcttcttt tgggtcagat actaactcgc cagtcttcat atctattgga agttgacctg
     5881 atgtagaaac tatgtttcca acttgaattg cttgagaata tggtccaatt gctcctggag
     5941 ctttctctgt agctattact acttttgaca tgacttcacc tcctattatt aagttatatt
     6001 tacaggaaaa taaaatttcc taaacaccaa tattaatacg aacctttaga ttaaatttca
     6061 aatctaaaac ttcaaataat caatacttag atgattatgt ttatttgtgt cttgttaact
     6121 tttattaaag caattaacgt gccaaacttt atttatatat aaaattttgt gaagtcattc
     6181 aaaatagtta cttctgaatt ttttcgtcaa aaaacgctct aaaattactt ttttatgatg
     6241 tcaatttttt tctcatatct agaaatttac caacatgaga attcttgttt tgagaattta
     6301 cagattaaag ttatttttta accttaaatt ctgaacctcc catgactaaa gtcacagggt
     6361 tcctgcttca tagaattttg cataatcaag ttattacata tgaaagaact atagttgaca
     6421 atcccactat tataaataat aaaaccttta cccatacctt atttacccta gttatcatag
     6481 agcctattga aaatataaca gctaaatata ataataacca aaaaattatc aatatctacc
     6541 actgttgctg aaaatccaaa aaactacctt ttgatagtat tacttaacaa tctaattaat
     6601 ctcataataa atttacaact tataaataaa ttgttatatt ggccgtatct caacaacttt
     6661 ctcctttcct agttttaaat cttttataga attgtaccac gtctaagtaa attttcatac
     6721 tattaagtct tatttctaat aaatcattag ctctcattct atgcccaata atgtaagatt
     6781 gcagtaattt tattgatttt aatagtttta tttaatatac ttatattaat tattttcata
     6841 tgaaattatt atttatatag ggggattttt ttaatatgag caaaagtgaa ttaacagcag
     6901 aaacaacaga agaaatgtta gaagtactaa gtggtaaaga ttatgatatt gcatgtcatt
     6961 tacatgaact tggtaaatca ttagattgta aaattgaacc aaaaacaggt gctcgttctt
     7021 acaaaatagt atattcaact aagaaaccaa aacgtagctt atttactatt gaatgtaatg
     7081 aaaaaaaatg gagagttaaa gcaaatcttt ttcatctaaa tacatataaa gatgctgtgg
     7141 aggaatgctc taaaactatt aaagatagta taactaaaac tcgtacttgt aagaaatgta
     7201 attcaaagtg tattggaggt tcttcgtttg aattagatgg aaagtcttac ctgacttgca
     7261 taggaagtgg tcattatttt gcaaatatgg aagaaatgga ttggaaaaac ctagaaaaat
     7321 taattactaa agaaaataat attatgcagg aatctgtata gttttttaag ggtaattatt
     7381 aaaatatatg agtaacaaca gatacttaaa taggcataaa aaaataaagg gtataataag
     7441 gatatagaat aaaaataaat gatatagcac aaagaggctg tctcaaaata cagataagcc
     7501 tctttttatt gttgaaatga taagaatata ttcaatttta tacaatttta tacaaacata
     7561 ctacgccaac ttacccattt catatgcctg tttcattgcc ttacttccct taatatcacc
     7621 tacattccaa gcacctacac catatactat acctttttcc ttagaaccat ttagacatga
     7681 tgtaaatcct ctaaatcctt ctattgttct ttccatatca gccacatttt catctgctgc
     7741 tgtaacgata aaatagaaat ctttatcaca aatttcagta tatctagagc aagttctatc
     7801 aatcatagtt ttcatttgcg cattcatagt ataaaaataa actggagtcg ctaaaacaat
     7861 cacatctgcc tcaatcattt tttcaagaat ttctcccatg tcatctgtct gtgaacaatt
     7921 tgcttctttt tcataacaag caccacatcc agtacaatag ttaatatctt tatcttttaa
     7981 aaatatcttc tccacttgat ttttagattc taatacccct tgtataaact ggtcacacaa
     8041 taaatcagaa tttccacctt ttctaggact agctgatact attaatacat ttttactcat
     8101 aagatactgc ctccattttc ttatttctac tgctctttaa tactttttct ttctcaataa
     8161 taccttcttc atatctatca atcttaaaat ttaatcgttc caaagttttt tgcatttctt
     8221 ctattctttt tgctagttgg tttctctgct ctattaaaag ttcttttcta gctttgatag
     8281 tactgctccc ttgttgaaac atagctacat actcaattaa tgtctcaata gaaagacctg
     8341 cacctctcat acactttatg aaattaaccc actcacaatc ttcttctgta aaagacctaa
     8401 ttccactttt atttctattt acaggtggta ttaaaccaat tcgttcataa tatcgtaaag
     8461 tatccgctga aagctcgtat tttttactaa cttctgaaat cgtcatacat atcttctcct
     8521 cttgatattg tatagattga attatttctc aaaggtttct gctacattct ttaaagcatc
     8581 tataatttta atgtgttgag gacagctttc ttcacattgt ttacattcaa tacaatctga
     8641 ggcctttcca taagttttag tataattatt atagtagacc atctgtgttg aaaatccagt
     8701 attaagagct tgtttttctg cattgtacaa cgcaaaatag tttggaatag cgatattttt
     8761 tggacagcct tctacacaat actgacaggc agtacaagga actaaaattg attcgttgat
     8821 aatctctact gcttcatcta taatattata ttcctcttgt acgaatggtt tgaaattttg
     8881 catatatcct gtattgtcta ataattgttc catatttgac atcccactta agaccatcat
     8941 aacattatct ttacttgctg caaaacgtat tgcccaagat gaaattgaca tatctggatt
     9001 gtagtcattc aataatttct ctgctttttc tggtatcttt gcaagtgtac ctccttttac
     9061 tggctccata acaattacag gcttattatg cttctctgcc acctcgtagc attttctaga
     9121 ttgtatgctt tcattatccc aatctaaata attaatctgt aattgcacga agtctacttc
     9181 tggatgctca ctcaagactt tatccaataa ttctgcacta tcatgaaaag aaaaaccaat
     9241 attctttatt tttccttctt ttttcttttc ttcaataaac ttaaagctat caaatttttt
     9301 agctacttca taatgtgaca cgcctatatt gtgtaaaaga tagtagtcaa aatagtctac
     9361 accacatttt tctaattgct cattaaaaat ggtttcttgc tcttcttttt tctttaaagc
     9421 cattaatgga agttttgtag ctatagtaaa actctctctt ttgtgtcttt ttactagaga
     9481 ttctctaagt gctatctcac tttttcccat gtgatacaca taggctgtat caaaatatgt
     9541 aaatcccctt tctaaaaaag tgtctaccat tttatttaaa tgttccatat caatagttgt
     9601 aacatcattt tcatctttta ttggtaaacg cattaatcca aatcctaatt tttttgcctc
     9661 cattgtttta cttccccctt taaaacttgt ttgttttttc tctctacttt aactattctt
     9721 acaagtatat aattcttgta taattatact ttactacttg gagttaactt caagtcaata
     9781 gattttttta tttttaagac aaatattata aaaatacagt tctttaatat taatccatta
     9841 catgtttctt tcaccctcca attctcaaac ttcacaaact ccaacatgta aagaaatata
     9901 tactttaaga aattcagtcc attaatatta ctacaaatac tttttagata tgtttaaaaa
     9961 gaaatttcac ctaaaatgtt tataaaagat ttagaaatag gtcttgtgtc t
//
LOCUS       DAKBNN010000143         9967 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000143 DAKBNN010000000
VERSION     DAKBNN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.144"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            121..1089
                     /locus_tag="OUB02_002825"
     CDS             121..1089
                     /locus_tag="OUB02_002825"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCT8886567.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1206..2336)
                     /locus_tag="OUB02_002826"
     CDS             complement(1206..2336)
                     /locus_tag="OUB02_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8886568.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2489..3442)
                     /gene="msrA"
                     /locus_tag="OUB02_002827"
     CDS             complement(2489..3442)
                     /gene="msrA"
                     /locus_tag="OUB02_002827"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCT8886569.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3566..4471)
                     /locus_tag="OUB02_002828"
     CDS             complement(3566..4471)
                     /locus_tag="OUB02_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCT8886570.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(4842..6434)
                     /gene="hcp"
                     /locus_tag="OUB02_002829"
                     /gene_synonym="priS"
     CDS             complement(4842..6434)
                     /gene="hcp"
                     /locus_tag="OUB02_002829"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCT8886571.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6494..7219)
                     /locus_tag="OUB02_002830"
     CDS             complement(6494..7219)
                     /locus_tag="OUB02_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8886572.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7384..8088
                     /locus_tag="OUB02_002831"
     CDS             7384..8088
                     /locus_tag="OUB02_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT8886573.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            complement(8252..9523)
                     /locus_tag="OUB02_002832"
                     /pseudo
     CDS             complement(8252..9523)
                     /locus_tag="OUB02_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         3319 a         1676 c         1157 g         3815 t
ORIGIN      
        1 tagtataaaa tcatattaaa taaattttct gaaataatag aatattaaaa tattcaaaaa
       61 cagcttttaa taacatctat aattttcaat gtatcgttat tttaggggga gtgatttaaa
      121 atggcaataa agccaagaaa aatatctatt gtaggttcag ggcatgttgg ttctcattgt
      181 ggatttagtt taataactca aggagtttgt gatgaactct ttatgattga tatagatgaa
      241 tctaaatcaa aagctcaagc tttagattta gctgatgcag tttcatatct gccacataaa
      301 gtacatatag aaaaaggaac tttttctgac tgtaaagatt ctgacatagt agtgataagt
      361 gttgcagact cttcagaagg gcctttacgt agacaaaaca caactagatt agatttacta
      421 agaccaacta ttggaatgat aaaatctata gtaaaaccca tagttgattc tggctttgat
      481 ggtatatttg tagtcatatc taatcctgtt gatgtagtta caaattatat ttgggaaaaa
      541 tctggctttc ctaaaaacaa agtcattgga actggaacag ctttagattc aacaagactt
      601 agaaggattt tatctgaaga aactggaatc gctcaacaat caattcaagc ttattctatg
      661 ggtgaacatg gagattctca aatggttcct tggtctcatg tttcaattgg tggtaaacca
      721 atcttagaca tgataaaaga caacccaagt acttatagca atttagactt accatcaata
      781 gttgaaaaaa ataaaaaaac aggaataagt ataataaatg gtaaagattg tacagaattt
      841 ggtataggta ctgctttagt agaaattgta aaagctatac tacacaatga gaaaaaagtt
      901 cttccagttt ctacattact agaaggtcaa tataacgaaa gaaatgtatt tgcaggtgtt
      961 ccatgtgtta taggaaaaga tggtattgaa gaaattattg aaatcaatat gactgaatat
     1021 gagcaaaacg aatttaataa atcttgttct gttttaaggg aatatattgc actttcaaaa
     1081 acactttaga ttcaatattc tttcttacac taaaagcagg taaatgactt attgaaaaga
     1141 cttttaacta agttaatgtt taaatcattt gtacctgctt ttaattttaa tactaaaata
     1201 taaacctatt ttttaccatc aaattttata actttacttt ttttatttac ctcttgagat
     1261 aacaatcctt ttttcaatcc catcaataat tctaagtctt ctggataaag atttattcca
     1321 aattcctcta aacttgatac tatactattt acactcatta ttttattttc tatcaataat
     1381 tcaactgctc tttttaaata cgttgggctt gaacctttca ttttgtctag tggttcttct
     1441 atcaaccagc cttttttatc catttcattc ataagataat tgtatttttt gtaatttatc
     1501 ttttctagat tatatgctct aaaaatcata gcagatatag gtacaatcca ttttttcttt
     1561 agttctacat aataatctaa atcttctgga tgagtcaaat cttttaaaaa actctctttt
     1621 ggcaataaaa atgcacaggc atactcatct tctgaaaatt gcttacttgg tattcttaat
     1681 tcacttgata ttatatatcc aagctcacat gccaaagtat aatttctaat tgatgctgac
     1741 tttttgtcat tacccagaga tattagatat ttagattctt tactaattct ctgtttttga
     1801 gtaaagatag ttgccccttt tctatctaca ttcatacctg atattataat ccctttagac
     1861 tctaacaaac ttaccatatt tactattgga gtactattta aatcccaaaa ctctctagtc
     1921 ttatatgcaa gagattctat atcatttata ttaatatctg acattacttt tctatttggc
     1981 aagttttctt ttggaaactt aatataattc tccataaatg aatatatttt atgtattata
     2041 attatttttt ctctataaga aatctcttct actctaggta gtttagattg agggttaaag
     2101 tggctatcct ctacaacgat atttatattt tcatttttat aaaaatactc ttttggaaaa
     2161 tttaatatat tagataattt taatgtattt tctatatttg gtacatactt attttcctca
     2221 aaagctttta aatctttttt atttatttta gtttctttag acaactgttc tatcgtcttt
     2281 gctctaaaaa ttctagctga ttttaatctc tttccattaa agtcttttaa attcatttgt
     2341 tttctctctt tcaaaattaa attcttaatc tattggtata aataagatta caattaataa
     2401 tttatcatac tattgacaaa aaaacaaaag gtgaaaatat ttttttatat tttcaccttt
     2461 tagataagtt tataataaat attcctaatt actcaaaaag ttttaaatat tctccatatc
     2521 ctaaagcttc catattttct cttggtataa atttaagaga agcagaattt atacaatatc
     2581 ttaaacctcc aagttctttt ggaccatcat caaatacatg acctaaatga gaatttcctt
     2641 tagcacttct tacttcaact ctgtacatac cttgtgagaa atcacttctc tcaatgatag
     2701 atttatctat aactggcttt gaaaaacttg gccacccaca tccagaatca aatttatctt
     2761 ttgaagaaaa taacacttct ccacttacaa tatcaacata aattccctct ttgttattat
     2821 cataatattc attttcaaaa ggtacttctg tcatatcatt ttgagtaact tcaaattgta
     2881 taggagttaa agtctttttt aactcttccc tatttatatt gtttccatcc caatgacttt
     2941 ttacaaaatt atatcttcca gaccctttat aatacatatt gtagtttaat ggtctttttt
     3001 tataataatc ctgatgattt tcttctgctt cgtagaattt agttgctgga attatatcag
     3061 tcactatttt actatcaaat attttactat tttccagatg ttctcttgac tctaaagcta
     3121 tatttttttg ttcttcatca tgataaaata tagcagtctt atattgagtt cccctatcac
     3181 caaattgtcc accatcatct gtaggattaa tctgtttcca gaagatattt agaagttcat
     3241 cataacttat tatatcatca tcatatgtta tttgaattgc ttctaaatga ccagtagttc
     3301 ctgaacatac ctcttgatat gtagggtttt caacatgacc acctgtatat ccagaaacta
     3361 ctttaataac tccatcatac ttatcaaaag gttttaccat acaccaaaaa catccacctg
     3421 caaaagtagc aagttttatc attttcatca ctccttaaat aaattatact ttatttattc
     3481 cctaagaagt aacttaataa tctgcatttt attattttta cctactcaat gaaatcatgt
     3541 gtaaaaatac aattgcaaat tgaatctaat ttctataaaa gctctttttc tttatccatc
     3601 caaaagcatc ttttatatca caattttctc ctaaagacat tagatattct aatgcatcac
     3661 aatcatccat agctacatct ttttccatct taggatttaa tctatagtaa ttatctccaa
     3721 gaagtttttg agaaaataac tgactcattt gagaatttcc ttctaaggta atagatataa
     3781 ttggtaaatc aggttcttta ttaattatcc agtcaatagc tccccattcg gtagtatctg
     3841 cctttatact gttatataca tatccagtcc ctattgaaaa aagtcttgta ttcttaagtc
     3901 taaatccact atcaatggca tgaattatac acgctaaact cgggtcagtt gctattattc
     3961 ctccatcaac atgacgatta tatgttggaa aaaataccgg agcagcacta cttgccattg
     4021 ctacatctac aactttgcta gtctttgtaa aagaatttgg catattgtta taaaatactg
     4081 gtttccatgc attttcctca tttcctatat aaaaacttgg tatcattact aatttattta
     4141 aatcttttaa ttctagttcc tctggaaata tacttaataa aacttctttt aagtttttgt
     4201 tttcatactt tggtctgagt atttctgcat aacttttatt aaaaatatac tttgaatttt
     4261 caattgagta aagttctttt atctctttag ggcttacacc atacgcaagc cctagtgcaa
     4321 ttagactccc tgtagatgta cctccaaaca tatttatatt gtttaataga tttggatact
     4381 cttttactat cctctcaagt atggatatac tcaatgcccc ttttaatcca ccaccatcaa
     4441 atgccaatat attaaaatta gaatttttca tatacatata cccccacata aaacacctta
     4501 tattttaaat tttaatcaaa ataatttcac tatagtatat atattttatg caagttacat
     4561 attacaatgt tcctctttta atatttaagt tacattcaaa cctttaatac ttaaaataaa
     4621 atatataaga tattatatat ctaagtatta taaatttaat acaaaaattc ttaaaagaat
     4681 atactgaaag caggtttatt tttcaattat tacaaaaaat aaataaaggg tcatctcaaa
     4741 attgattttt taatcaaatt ttaagtttaa ccccttatct taattgtggc atacttaaaa
     4801 atattttaac tataaatatc tttaagtata gatatattta tttatcctaa aatcttcttc
     4861 atatcttctt caggagtaga tataggtgtc aatgcaaatt tatcaaccaa tatatctaat
     4921 acattcttag ataagaatgc tggcaaagaa ggccctatat agatattttt gatacctaaa
     4981 tgaagtagtg taagaagtat acaaacagct ttctgctcat accatgacag taccatgcta
     5041 agaggcaaat catttactcc acattcaaaa gcatttgcca atgaaactgc cactttaatt
     5101 gcactataag catcattaca ttgtcccata tccattaaac gaggtaagcc acctatatcc
     5161 ccaaggtcta aatcattaaa tctatatttt ccacatgcaa gtgtcaatat aacagtgtct
     5221 ttaggagctt gttttacaaa ttcagtgtaa taatttcttc caggttttgc accatcacaa
     5281 cctccaacca ataaaaagtg tcttatagct ccactcttta cagcatctac tactttatct
     5341 tgtacagatg aaactgtacc tcttgcaaaa ccagtagtta atgtatctcc tccattaata
     5401 ccagtcatat gaacatcttc tctatatcct ccaagttcaa gagctttttc tattacacta
     5461 gaaaaatctt tatcttcatc aatatgaacc atacctggat aagcaactac ttctgtagta
     5521 aatactctat ctctataact atctttaaca ggcattaaac agtttgttgt aaataatatt
     5581 ggagcaggaa tattatcaaa ttctttttgc tggttttgcc atgctgtacc aaaattacct
     5641 tttagatgtt tatattcttt tagttttgga tatgcatgtg ctggtaataa ctcaccatga
     5701 gtatatatat ttataccctt atctttcgtt tgttctaata atttgtgtaa atcataaagg
     5761 tcatgtcctg taactacaat aaatggccct ttctcgattt tcatagatac ctgagtcggt
     5821 tctggtgttc cataagtttc agtatttgct ctatcaagca attccataca ttttaagttt
     5881 atttctccag ttttcataac tagacctaaa agttcgtcaa tagacaagtc tcctccaact
     5941 gctattaatc cttcatagaa aaattcattt acgtctttgt ctgtataacc aagtaccatt
     6001 gcatgatatg cataggcagc catacctctt aatccaaata agattaatga ttttaatgaa
     6061 cgtatgtcct cattatcatt ccataaatcc ttcatatcat agttatcatt tctattacaa
     6121 gttgttgaac aatctgaaca tctaggtaca agcttacttt tttctacctc agttttttca
     6181 atttgtcttt ttatagcttc atcatcaaaa cttacattcg ttatagtagt gaatagcccc
     6241 tctatcataa ttttatctgt ttcacttgtt ggtctattct tatttccaat agttgctctt
     6301 gaaagaccaa tcaacactcc tgtaagttcg tcttgtaagt tagctgtacc ccccttttta
     6361 ccacagacac catttatagt acaaccttta cctcctgctg tttgttcaca ttgaaaacaa
     6421 aacatttttt ccatttttta ttcctccctt tttatttatt aaaatttatt atatttttct
     6481 ataattcact taattaaatt tctccatcta caatttttcc atctacagat atagtaacta
     6541 tttgccatgg tatcattttt ccactatttt gtagagctgt cttaacagca tttactatac
     6601 caccacagca aggaacttcc atacgtgtta ctactatatt tttaatatca ttttctttta
     6661 aaatctctgt aagtttttca gcatagtcaa cttcatcaag tttagggcat ccgattaatg
     6721 taaccttatt tttcataaat ttattatgaa aatttccata tgcatacgct gtacagtctg
     6781 ctgctattaa aagacttgca ttctttaaat atgatgcatt agttggtact aattttattt
     6841 gtactggcca ttgattaagt tgagaacctt ttatctctac tatatcattg cttatagacg
     6901 tactatttga aacctctcta tttattgact tagactgaga acctggacat ccacatgcta
     6961 aagttttctt ttgtgctttc tttgcttcca tatttgcttt tactgcagct tcatcatatt
     7021 ctgctgcttc tctatactca aatgaaatcg catttgttgg acaagttggc agacaatctc
     7081 ccagcccatc acaatagtca tctcttaaaa gttttgcttt tccatttacc attcctatag
     7141 cttcttcatg acaagcttca acacatagtc cacaaccatt acacttttcc tcatcaattt
     7201 taattatttt tctcatcatt tatatcacct cataaaattt ttttaattta aagtattgat
     7261 ttcttatata tttattttta atacctatat ttctttattt caaatttctt gtagtaggat
     7321 ttttatatcc ccttgctttt ataaacttag tatagtacaa tgaaaacaaa ttatcggttg
     7381 catatgcaac aaggaagtga ttatttgaaa aaatttttag aaaagcatat agacctactt
     7441 aaaaaaattc ctctatttga aggaattaaa agtgatgaac ttgaagaaat gttggaatgt
     7501 ttaggtgttg tagataagac ttacccaaaa gatagtataa tattctctgt tggaagtgaa
     7561 gttacttcta ttggaattat gcttaaagga agtgctcata taataaagga agatatcgaa
     7621 gggaatagga atattgtagc tgaactcttg cctggagatt tatttggaga ggtattcgca
     7681 tgtactagac tacataaaag taatgttaca gtaaccacaa catcttcttg cgaagtttta
     7741 tttataaagt ttaaaagtgt aactggtatt tgttcatctg catgtgtatt tcataatagg
     7801 cttgttgaaa atatgctaca attaatagct gaaaaaaaca ttttacttca caataaaata
     7861 gaattacttt ctcgtaaaac aactcgcgag aaattgctta tgtatttttc aaaacagata
     7921 gaacaaacag gtagccacca gtttacaatt cccttttcaa gaaatgaaat ggctgatttt
     7981 ttatgtgttg acagaagttc tatgtcaaga gagcttggca agatgcgaga tgaaggcttg
     8041 ttatctttta acaaaaataa atttgaaatc ttttatttta aaaaataaaa tagatattta
     8101 aacatattta tttcaatata tactatatta gttgattcaa attataaata tcatatttga
     8161 atcttaaatt tatattttta cttaaaaagt taataggtat ctaatttatt aaaattaaga
     8221 tacctattaa ctttattaat taagtgtaca attagcttat tttctttgta gtaattgctg
     8281 ttttatctat aagccaatct tttccttcta ctaatcttga aactaacatt gatgatactg
     8341 tatttccagt tgtatttaat atagtattag gaatatcagt tatagtagtt ataactgtta
     8401 acagtggaat tgcctccact ggaaaaccga agatgctaac cacgagcatc tcaccaataa
     8461 gtcagccacc tgctacagga gccataaata ctccaacaac aaatacagtt ataaataatt
     8521 gacctatcgt tgctggattt gccattccac tacctaaaat acctgataaa aatgccactt
     8581 ttattattac tgctaaaact gacccatctt tatgtatatt tattccaaga ggtataactg
     8641 tttctgatat atcatcagga actcctatat ttttactagc ttctaaattt gatggtatac
     8701 atgctgaact tgaagaagta gctagtgccg ttgctattgg ttttatagca tttctccaga
     8761 attgtctaaa cccttgtttt cctgcagata aaaaagcata cagactgtaa aataataaga
     8821 aatacatggc tgctgttagt gaataaatta caaacaccct tacatatcct tgaattattt
     8881 tacttccaag ttccccaatt attgtagctg tataacagcc tagccctata ggagcataaa
     8941 gcatgaatat acttactact tttaatattg cttccgttcc ttctgataaa agctttctta
     9001 ttgtatttcc cctatctccc atcatagcta ttgctgttcc aattaaaatt gcaaatataa
     9061 ctgattgtag taaattacta ctggaaaata aatctccaaa atttttaact gtaatacttg
     9121 atactacttg gtctaataac gttccttttg attctaaagt ttctgcatct tgagccacca
     9181 ttaaattttt tacacttgat acatctaatc ctttagacgg attaaataat aatgcagatg
     9241 taatacctaa aaatgcaatt acaagtgttg ttcccataaa tactatagct gtacccttca
     9301 ttatttttcc aaatcgttcc atatttttta tactagcaac acctgaagaa acactaagaa
     9361 aaactaatgg tactactatc attaatagag catttatgaa taaatctcca aatggtttta
     9421 gtattgaagc ttttggacca agtactaatc ctactatcgc ccctactata gtacatgcta
     9481 aaagtataat tgatgactta taatttttta aaaagtccct catttatatt ttcctttcta
     9541 atgctttata ttataaatta ccccaaatgc taaaaaaatt aaagtaaatt atctaatgcg
     9601 cattaataat ttttgtaatt ttccccaaca gctactttag caaatactgg aatatatgtc
     9661 aatttctata gtttttttgt atttttaacg ttttattaat agttcatatc tatatataaa
     9721 tattaataaa attctgcttt taaaactttt tttctagatt aattttaata aaacaaacaa
     9781 tataaaaaaa tatatataat attacttaat tattttcaaa tttaatactt tgtactttaa
     9841 tataaaattt tagtatattc attgtaattc ctatactata taactccata aagttgattt
     9901 tgtaaacaca tatgatactt tgtcaaatat tattttttta tgtatttgat attttgatat
     9961 actttta
//
LOCUS       DAKBNN010000144         9883 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000144 DAKBNN010000000
VERSION     DAKBNN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.145"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            151..630
                     /locus_tag="OUB02_002833"
     CDS             151..630
                     /locus_tag="OUB02_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCT8886574.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
     gene            1261..1638
                     /locus_tag="OUB02_002834"
     CDS             1261..1638
                     /locus_tag="OUB02_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8886575.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            1631..2664
                     /locus_tag="OUB02_002835"
                     /pseudo
     CDS             1631..2664
                     /locus_tag="OUB02_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
     gene            2730..3317
                     /locus_tag="OUB02_002836"
     CDS             2730..3317
                     /locus_tag="OUB02_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCT8886576.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(3513..4425)
                     /locus_tag="OUB02_002837"
                     /pseudo
     CDS             complement(3513..4425)
                     /locus_tag="OUB02_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4928..5305
                     /locus_tag="OUB02_002838"
     CDS             4928..5305
                     /locus_tag="OUB02_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8886577.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            5298..6332
                     /locus_tag="OUB02_002839"
     CDS             5298..6332
                     /locus_tag="OUB02_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCT8886578.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKIYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKDPKENTVGNNITQIDISIDNDFMLYLVYLWLFVTI
                     AIAVYTFIEYTKFKNLVVDVSYDIEDNDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            6383..6985
                     /locus_tag="OUB02_002840"
     CDS             6383..6985
                     /locus_tag="OUB02_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCT8886579.1"
                     /translation="MKSLKKILIVTGCLTLISTSLVFASTNNDVNVSKSNEEQTTNSF
                     TITGYEFIYENAPQPIKSDYEKKCKELNINPSKDDTIFINEKFIDSYNINIDDYNIEP
                     KAFIIRYKKDLNRFDVTGPISYSVNINTTVVGYGHVEKGNPVHLVQLLCQQINGSDID
                     PDSKFGPATYNQVKILQGRLGVTKDGVVGKATWQAAGERY"
     gene            complement(7070..8092)
                     /locus_tag="OUB02_002841"
     CDS             complement(7070..8092)
                     /locus_tag="OUB02_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCT8886580.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKIRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(8421..8603)
                     /locus_tag="OUB02_002842"
     CDS             complement(8421..8603)
                     /locus_tag="OUB02_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886581.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            8916..9335
                     /locus_tag="OUB02_002843"
     CDS             8916..9335
                     /locus_tag="OUB02_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HCT8886582.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            9466..>9883
                     /locus_tag="OUB02_002844"
     CDS             9466..>9883
                     /locus_tag="OUB02_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8886583.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQ"
BASE COUNT         3829 a         1053 c         1372 g         3629 t
ORIGIN      
        1 aaaaaatgta ttttaaaggc ttttattata ctctttttta tttgcataat taacaataaa
       61 aatatattgt taattataat atttttattg aaaaacgata taatataatg tattatacta
      121 tatataaaat attttaaagg agttggcatg atgtctaata aacttataaa aaatttacta
      181 agtggtatat tacaaatttt attttttctt ttaggattag taattgttgc aggaggattt
      241 aaaagtttta tgtacctctg ttttagtgga gaagcaactc tacaaggtac tatttatgga
      301 atattaatgt ttattttagg tgtatcatat tttatcatta taaaatcatt gatagaagta
      361 ttaggtagtt ctgaatatag cctatttgta aaagagaatg taaaaagatt taggattatt
      421 ggatatcttt tgcttttaaa ctctctaatt gagtttattt caacttttgg tactactgga
      481 aaaggaatga gatttctaga tttaggattt ggattttatt ttactgttcc agtatttgta
      541 tattttataa cttctctgat gagttttgta attgcagatg gatttgtaaa ggcaataaag
      601 ataaaagagg ataatgattt aactatttag gtgtgacact ttaatatata ggagataaat
      661 aacccgaaaa gagtgattaa ctagtaattt aaaatatatt agggtataat acataaacta
      721 taatgctaaa attaaaagat tataatacta aaatataatt atgttttaat taaaataaag
      781 aactttatta gtagatatta aaagcacatt ttttataaaa gtgctttttt ataatttatg
      841 aaaggtataa ttataaaagt cttattcaat aaaatttata attaatttag atatatttgt
      901 aaataatcag tataaaatag atgttttaat agcataaagg cataattaga aggttattaa
      961 aatttattag atattactct agtttatttt aaaaaaagta ttataatatt ccttgtgtga
     1021 aaatatagga atttttatta aacagtttta atgtgtaaat aacattctac actattgtaa
     1081 ttaaatatgt aaaaagtaaa atatttaaat agatatattt taaaatctaa cttttaatat
     1141 tgtaaaaaaa ttagaaattt aacaaaaagt acaaaaattc acattttttt cataaagtgt
     1201 ttgactaaaa aattcacatg ttgtagaatt aaggtagcag gagttttgag ggaggtgtaa
     1261 ataatgataa gaaaaatacc acaagcagaa ctaaaagtaa tgaaattcat ttggaaagta
     1321 gatgttacag tgacatcaaa agatgttatt gaagctatgg aacaaaaata tggctggaaa
     1381 caaaccacca cactgacact tttatctagg ctggtaaaaa gaggattctt agatgctcaa
     1441 aaaatagaca gatatacaca ttacacaata agagtaaaac acaaagatta tttaagtttt
     1501 gagacaaagg atttcctaag taatatacat gatgactctt taagaagctt aatttcagca
     1561 ttacatgatg atgaaaatat aggtaaagat aagctagatt ttctagaaag ttattttgat
     1621 aatttaaagg atgaataatt atatgctata tgtttttgaa aatcttataa agacaactat
     1681 tgtatgtagt cttggtattt gtctattatt atttttgaaa agatatttat ttaagaagtt
     1741 tagtaagaga ttcaattatt atatttggtt gataatagtc tttagaatgt tactttttct
     1801 atttaattat acaatagtat atgaagtaaa gaacccaaag aaaatgcagt aagaaataat
     1861 attacacaaa ttgatatttc tacagataat aatcttatgc tgtatgtagc ttatttatgg
     1921 ttatttgtaa ctatagtcat tgcagtttat acatttataa agtatacaag atttaaaaat
     1981 ctagttgttg atgtatctta tgatattgaa gataatgata taaactgtat ctacaaaaac
     2041 ttgttaaaag aattaaatat aaaaaagaaa attgaattaa gaggttctga tgaattggta
     2101 agtccagcag gcatgggatt atttaaatct tacatattct taccagacta tccttatagt
     2161 aaagatgaac taacttggat tttgaaacat gaattaatgc attttaaaaa taaagatatt
     2221 ttaataaagt ttttagtttt gtctgttaga ataatatatt ggtttaatcc acttgtatat
     2281 gtaatgagca ataaagtaaa tctagattgt gaattatgct gtgatgaaag tgttttgatt
     2341 gattgttctt taaagaataa gaaggagtat gcattagcac ttataaagtc aattaaactt
     2401 agtaagaatt ataatagtgg tattttaaca acagagttta ataagacaag tttggaaaag
     2461 agacttgaca gtattgttaa gaaaaaaggt aaaagtggaa tattggtgtt attgattttg
     2521 attacattat tttcagttac atattttaat tttgaaagta ttagttttga taattcaaat
     2581 tctttaagga atatggggat tttacaaaaa tcaggagtag atcttaagga agaagatgat
     2641 attagcatga gagttataaa ttaatagatt aatatatgta atgaaaaggt atattgtcat
     2701 attattaata aattaggagg aattatgaaa tgaaaaattt aaaaaaaatt ttaataatta
     2761 caggttgtct aactctaata tctactagtt tggtatttgc aaatactact actgatgtag
     2821 atacatcaaa ggatgatgat tcaataataa gttcatgtac tataactggt tatgaattta
     2881 catatgaaaa cgcaccacaa tctgtaaaat ttgattatga aaaaaaatgt gaagcgctaa
     2941 atataaaacc atctaaaaat gatataattt ttgttaacaa agattctata gatgcaaatg
     3001 atgtagaaaa ctatgttgag ccaaaagcat atattattag atataaaaaa ggttatgttt
     3061 ttgaagttac tggacctaag agttatacat taaatgtaag taatactgta gtcggatata
     3121 atcgtgttga atcaggtaat cctgtgcatt tagttcaact tttatgtaga accttagatg
     3181 gttcaagtat tgatgatgat agtgaatttg gacctgctac atataatgca gttaagtcac
     3241 ttcaaggaag acttggatta acaagagatg gaattgttgg aagaagtact tgggatgcag
     3301 ctggaagacg tctttaaaaa gaaagctatt gcacagataa ttttaaattg ctgaatctgt
     3361 aaacatttac atgtattctt taaacaaaaa tttatttttt aagtgataat taaattttta
     3421 ctaatagggc tatggcttta aaaatcatag ccttattttg atatattttt catttttgca
     3481 aaaactatta accctttaag aaaaattctt gtttattttg aaaaatttct ttcttcaaaa
     3541 taaatactta aatgagctaa aatttgagca caatttctaa ttttctgagt ccatttttaa
     3601 gaaatataca tggttgttaa gtatagtgat gtgtgcaaat aattatccaa agtaaaaata
     3661 gatttcaatt tagtaacttt tcttaataat ctattctaac tttacattga atttgtagta
     3721 tatatcgatg tatttatttc ttgaggatac ttaaaatatg ttactaattc attccaattg
     3781 tttcaccaac ctcttaatac tattgtatat tgattgtact atttcatatt attaaaaccc
     3841 cttttatgcc ttctaagtta atacatatag ctatatatac tgttttgttt attattccac
     3901 ctcacttcta actttataat gtatagcatc tataaataca attgaataaa ttatattaag
     3961 aggtctaagt taccatttat gagctaaagg aataacttta tcagtagttt tagttattag
     4021 tgaatgagat aatatcaatt ccataagtac ttttaagatg acttaatata ttttgagtag
     4081 acatgccttt taagtatatt ccaatcactt gattttttat tcctaatata tcattttcat
     4141 agtttctaat tacctttagt tcaaaatctt catttcgctt tcttgaaata tttaaagtta
     4201 tttctccaaa atctgattta atatttttat ttctatatcc atttctagag tttttagtat
     4261 ttttgttctt attatcgttc tatcataacc taaaagttca tcaagctccg cttctaacat
     4321 ttaatggatt gtactttaaa ataaatcttt tagtatatct tgaatatctg taattgtttt
     4381 gacatcatat tcttcaataa atttttttaa tattttagtt tttattaaac ataaaaagtg
     4441 tacatcttcc tttgtatagt agtgatgttt taattattat aactttttag aagaatacaa
     4501 actttatttt aaagacactc aaaatggtgt ttttatacct ttatttgata tgtataattt
     4561 tacttataac ataaacttat ataaaacaga tattttaata acacaaaggt acaattagga
     4621 gattgttaaa atttattaga tattactcta gtttatttaa ataaaagtat tataatattc
     4681 cttgtgtaaa aatataggta gttttgttaa ataattttaa tgtggaaata acattctaca
     4741 ctattataat taaatacgta aaaagcaaaa tacttgaata actatatttt aaaatctaac
     4801 ttttaatatt taaaaaaatt agaaaattaa taaaaagtac aaaaattcac attttttcat
     4861 aaagcgtttg actaaaaaat ttacatattg tagacttaaa gtagcaagag ttttgaggga
     4921 ggtgtaaatg atgataagaa aaataccaca agcagaacta aaagtaatga aatttatttg
     4981 gaaagtagat gctacagtga catcaaaaga tgttattgaa gttatggaac aaaaatatgg
     5041 ttggaaacaa accactacac taacactttt atctaggtta gtaaaaagag gattcttaga
     5101 tgctcaaaaa atagacagat atacacatta cacagtaata gtaaaacaca aagattattt
     5161 aagttttgag acaaaggatt tcctaagtaa tatacatgat gattctttga aaagcttaat
     5221 ttcagcattg catgatgatg aaaatatagg taaagacacg ctagattttt tagaaagtta
     5281 ttttgataat ttaaagaatg aataattata tgctatatgt ttttgacaat cttataaaga
     5341 caactattgt atgtagtttt ggtatttgtc tattattatt tttgaaaata tattttttta
     5401 agaggtttag taagagattt aattattata tttggttggt aatagtcttt agaatgttac
     5461 tttttctatt taattataca gtaatgtatg aagtaaaaga tcccaaagaa aatacagtag
     5521 gaaataatat tacacaaatt gatatttcta tagataatga ttttatgctg tatttagttt
     5581 atttatggtt atttgtaact atagccattg cagtttatac atttatagaa tacacaaaat
     5641 ttaaaaatct cgttgttgat gtatcttatg atattgaaga taatgatgta aattgtatct
     5701 ataaaaattt gttaaaagaa ttagacataa aaaagaaaat tgagttaagg ggttctgatg
     5761 aattgataag tccagcaggt atgggattat ttaaatcgta catattctta ccagactata
     5821 cttatagtaa agatgagtta acttggattt tgaaacatga attaatgcat tttaaaaaca
     5881 aagatatttt gataaagttt ttagttttgt ctgttaaaat aatgtattgg tttaatccac
     5941 ttgtttatat aatgagcaat aaagtaaatc tagattgtga attatgttgt gatgaaagtg
     6001 ttttgactga ttgctcttta aaagataaga aagcatatgc gttagcactt ataaagtcaa
     6061 ttaaacttag taagaattat aatagtggta ttttaacaac tgaatttaat aagacaaatt
     6121 tggaaaagag acttgatagt attattaaga aaaaagggaa aagtggaata ttgatgttat
     6181 tgattttgtt tacattattt tcagttacat attttaattt tgaaagtatt agttttgata
     6241 attcaaatta tttaagaaat atagggattt tagaaaaaac aggcgcagat cttaaggaag
     6301 aagatgatat tagcatgaga gttgtaaatt aatagatttt tatagtatta taatttatta
     6361 acaaattagg aggaattttg aaatgaaaag tctaaagaaa attttaatag tcacagggtg
     6421 tttgactcta atatctacta gtttagtatt tgcaagtact aataatgatg taaatgtatc
     6481 aaaaagtaat gaagagcaaa caacaaattc atttacaata actggttatg aatttattta
     6541 tgagaatgca ccacaaccta taaaatctga ttatgaaaaa aaatgtaaag aattaaatat
     6601 aaatccatct aaagatgata caatctttat taatgaaaaa tttatagatt catataatat
     6661 taatatagat gattataata ttgaaccaaa agcatttatt attagatata aaaaagattt
     6721 aaatcgtttt gatgttactg gtcctataag ttatagcgta aatataaata ccactgtagt
     6781 aggatatgga catgtagaaa aagggaatcc tgtgcattta gtacaacttt tatgtcaaca
     6841 aataaatggt tctgatattg atccagatag taaatttgga cctgctacat ataatcaagt
     6901 aaaaatactt caaggaagac ttggtgtaac aaaagatggt gttgttggaa aagccacttg
     6961 gcaagctgcc ggagagcgtt attagaaaag aggttgtcgc actaagaaat agggcgacaa
     7021 cctttttata tagaatatat taatcagtat cttagagtct agaaatattt tacaataaat
     7081 tccatataac tctaccatct atatccttaa cattttgaca accagtcaaa atcatagtct
     7141 cacataactc attcctaatc ttctctataa aaaactccac accatcaaga ccaccgccaa
     7201 aagaagcagt aacaaaaggt cttcccatca aaacagcatc agcacccaat ccaagcatct
     7261 taactacatc aacaccagtc ctaactccac catcaacaag tattgttatc tttcccttta
     7321 cagacttagc tatatctgga agtacctcac aagtaccagg agtataatca agtactcttc
     7381 caccatgatt tgaaacaata atggcactag caccagactc tacagccatc aaagcatcat
     7441 ctacagtcat aattcctttt ataataaaag gtaatctagt ggattcaacc aattctctaa
     7501 tctcatcaat tgttttagga gaaaaaggat tttcttgaaa ttgattatta ataagtccac
     7561 aagcatcaag gtctacacca actgcaaaag cacctgcttc ttcagacaat ctaatttttt
     7621 ctattatctt tgaattattc caaggcttta taaatacaat tccatttccc ctattatcct
     7681 tcaatacatc taaattatcc agtaaaaaag tatctacatt agtatcgcca accatagcat
     7741 aaattccaga attagaacaa cctctaacaa caggctctat gtattctttt tcggaaactt
     7801 taccacccat gttcaatata gtacctgata caggtgcagc gaatatagga gaactcattt
     7861 ttctaccaaa taattctatg gatgtatctg gctttgaaac attatgtatg actctcatat
     7921 ttatctttat tttttctaga ctttttctat tttcaatgaa agacgaacca cttcctttac
     7981 cacccatacc aggaacatct ccagcacaag ctaatccatt acaaatttta cataccttac
     8041 atgttccatt aaagttctct ctagctgatt ttagtaagtc attgtaattc atacaaaatc
     8101 tctccttttt ataatattaa aattgagtat tactatattg aaaatatctt aattgtaaat
     8161 atatggatgt tattatgatg agctaaacta gataatacat attagatatt ttttattgtt
     8221 gtagatagtt aaattatatc atattagaca gtcaaatttt gaaaaaaact aacttattta
     8281 agtatacatt ttgaaaaaat taaaaaagct atatatagat ttttaaagaa atctatatat
     8341 agcttttaag tttatgtcaa tcttttttgt taatgaataa ataatatagg atacaatata
     8401 caaaaaagtt tcttgaaatg ctaagataat ccccatttac aaagcaacat cattatactg
     8461 tgtaaatttg aatcaacaat gttttcacca tatttcatca taaaatcctc taattgtgtg
     8521 tcaataaatg aataatatcc acttatacta tcgatgtatg cagtcattaa gtgtatacat
     8581 tcatcacttt tatctttata catatatatc tccccctata tacaatatat aaatttagat
     8641 accaaatcgt atcatattaa atagttaagt gtgtaacatc tatgcagtta tatatttata
     8701 tcattatatt aataactaaa gtgagttttg acaatagatt ttgggaaact ggatgttcaa
     8761 attgcaaact tggattttaa aatcattaat taagatagcg cataggaata tatgtataca
     8821 taacttaatt ataaagatta taaacaatta cttttattaa ctttatttaa atggtagaat
     8881 aacttggaga ttaataatta taaggaggat ataatatgag tttatataag tttatatatg
     8941 atgataagga atatttatta aaagaggaga actgtagtgc tttaataaat gatgaagaaa
     9001 atccagttca aggagtaagt atttcaaaaa taattgatat acttaatgaa gcagaagaag
     9061 ttgattttga tgtagaatat tatcaagaag catgtccatt atgtttagaa ggagtaaaag
     9121 aaaagaaaaa attctttcct tttttagaat atcactttta tattttcagt aaagatggag
     9181 aatatgtaat aagcaatata tctgttgatt ataaaggact atcttttaat aaactatcaa
     9241 gggcaaataa agttgataat agttatattg taagtgtgat aatttgcgaa aattgtcagg
     9301 attatatagt tcagatagaa aattgtatag tttagtgtaa agtatgtctt aatttaagag
     9361 aaatttctaa attgagacat caactttttt caaaaatagt atggaaaatt actaaattta
     9421 tgagataatg taagtcggag gtgagttaca atatatagta aatatttgga agagatagat
     9481 gatacaattt tagattcaat gtttaaacta accttgaatg aaagattatt gactaataac
     9541 gattttattg aaaataaaat tgatgtagta aagaatttaa ttgattgcta taaaactgaa
     9601 caaaatagtt taatgttatt aaaatcaaat tttttgctag ctttattata tggtattcaa
     9661 gggtttagtg aaaaaatgaa agagtatatt ttgatttcct gtaaatatat tgataaatgc
     9721 ttacccaaag attataaatt tttagcacga ttttattcac aaatagctat tttaagttta
     9781 aaaaatgaag atgtaaataa ggcaaattta tatatggata aatttaatct tatttgtgat
     9841 gaaaataatt ttctaattga agaaattatt tttaaatcac aac
//
LOCUS       DAKBNN010000145         9852 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000145 DAKBNN010000000
VERSION     DAKBNN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.146"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..2190
                     /locus_tag="OUB02_002845"
     CDS             <1..2190
                     /locus_tag="OUB02_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8886584.1"
                     /translation="VLSKELDSRNFKLKTLNEIDFFSKIPNREFMYQHIDDCISKNIH
                     IGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKGIVAYLKGEGFLIVLP
                     NVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTIWNGEPNMTTLMLFNQ
                     VYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHVLQTKQIEVFYQPLYN
                     IKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDLHLFEENCKNIRRWID
                     NNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIEITENILIENTNTIIQ
                     QLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLSHNLNDFKNTTILYSI
                     INIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPMECKEFEKLLSKENNSK
                     KMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDFYNKTEITSFPKVIEH
                     FEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQKYSNFYFSANCIISE
                     NKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQFYSMLPVGIIRYDLF
                     GDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFVYKKSLDMIDTNKIEP
                     FLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKNLTKI"
     gene            2221..3066
                     /locus_tag="OUB02_002846"
     CDS             2221..3066
                     /locus_tag="OUB02_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8886585.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3306..5039
                     /locus_tag="OUB02_002847"
     CDS             3306..5039
                     /locus_tag="OUB02_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8886586.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5431..6314
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5431..5459
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6844..6948)
                     /locus_tag="OUB02_002848"
     CDS             complement(6844..6948)
                     /locus_tag="OUB02_002848"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCT8886587.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7573..7818
                     /locus_tag="OUB02_002849"
     CDS             7573..7818
                     /locus_tag="OUB02_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886588.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIISFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8571..8717)
                     /locus_tag="OUB02_002850"
     CDS             complement(8571..8717)
                     /locus_tag="OUB02_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886589.1"
                     /translation="MVFETFDKAIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8750..9094
                     /locus_tag="OUB02_002851"
     CDS             8750..9094
                     /locus_tag="OUB02_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886590.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSTKNQVDFIKLVLKESYRLKEISENI
                     CDFVTTPIKKIV"
BASE COUNT         3901 a         1223 c         1249 g         3479 t
ORIGIN      
        1 gtattatcta aggaattaga tagtagaaat tttaaattaa aaaccttaaa tgaaatagat
       61 tttttttcta aaataccaaa cagagaattt atgtaccaac atattgatga ctgtatttcg
      121 aaaaatatac atataggaat catgttatta aatatcgata actttaaatt atttaatgat
      181 atatatggat atctagtcgg agaaaattta ttaattactc taactcaaaa cctattgcac
      241 tttattccag aatcaaaggg aatagtcgct tatttaaaag gagaaggttt tttaattgta
      301 cttccaaatg tatcaaataa agagttaaat aatatgggtg caactattat agagagcgcc
      361 cgagaattaa aaataaaact tcctaatgaa aaagaaatat actccaatgt tacatttagt
      421 atcggcagca ctatctggaa tggtgaacca aacatgacta ctttgatgct tttcaaccaa
      481 gtatatagtg ctttaaaaaa ggctaaaagt gaaggcaaaa atagatacat tctatttgat
      541 tttaaagata atagttttta caataaatta gaaaatgata aggagttctt atcagaaata
      601 tcacatgttc tacaaactaa acaaattgaa gtattttacc aaccacttta taatataaaa
      661 aataatacta tagttggatg tgaggcactt gcaagatgga gacaccctat taaaggactt
      721 cttactccag ataaatttat tcctttattt gaacagtttg gtctgataac ggaattagat
      781 ttgcatttat ttgaagagaa ctgtaaaaat ataagaagat ggattgataa caatgatgtt
      841 tttgttccaa tttgttgtaa cttttctcgt cttcattttt ttaaccctaa ttttccaagt
      901 atgctaaaag gtattactga aaagtacagc gtctctacat caaattttag cattgaaata
      961 acagaaaata tattaataga aaatacaaat acaattattc aacaactagc tgaacttcgt
     1021 tcttatggtt tttctgtatc aatggacgat tttggttctg gatattcatc ttttggtatg
     1081 ttgcaaaatc taccttttga tataatcaaa attgatagga tatttttatc tcataattta
     1141 aatgatttta aaaatacaac cattttgtac tctataataa acattgcaag agctcttggg
     1201 atgcttacag tatgtgaagg tgttgaaaca gaacaacatg tagaatttat taaaagtata
     1261 aattgcgata tcgcccaagg ttattattat gcaaaaccca tggagtgcaa agaatttgaa
     1321 aaattactat ctaaagagaa taattcaaag aaaatgttta taaaatattc tgatatagga
     1381 aaatctttaa tagagaagat atttgatgga ttttttatta tgcaagatat gaaaaaacta
     1441 agagctaatg tcactgaaga cattcaatgg tttgattttt acaacaaaac tgaaattaca
     1501 tcttttccta aagtaataga acattttgaa aaacatatta aaggtaaaaa attttctatt
     1561 ctttacaaaa gtataacccc acataatact gaatcagatt cattattaat tagtggagaa
     1621 gccgttttat cagatgaagc ttctgaacat caaaaatata gcaatttcta ctttagtgca
     1681 aattgtataa tttcagaaaa taaattactc ttagcaaaat tacatttgga tttaattaaa
     1741 actgctggct atataaatga atttgaatca tattcaatac ctaaaatgac aaatacacat
     1801 cttgatgaaa gtaatatttt atcacaattt tattcaatgt tgcctgtagg tataattcgc
     1861 tacgatttat ttggtgatat gattattact tatatgaatg aagaaatgtt tgatattata
     1921 ggttatacaa aagaacagtt ttttggtgag atgggcggaa atttaagagc aattgttcat
     1981 ccagatgatt tagactttgt ttataaaaaa agtttggata tgattgacac aaataaaatt
     2041 gaaccatttt tatatagatt cataaaacga gatggtcaaa ttgtcacagt tatgtataca
     2101 caatgtaacc tttctgcaat tgatggaagg tcgattgccc agggtatgta tgtagatgta
     2161 aataaaataa aaaatttaac aaaaatctag tacaaagtat tttattttgg agaaattaat
     2221 atgtttaaag aaattttttt aagaactttt ccaggatttc taattataag agattctaac
     2281 tacagaatta tatttataaa tgataattta aaaaatttaa ttaaaagtta tatgcaagat
     2341 aatccacttg gaatgactaa tatagaaata gctaagaaac tccctgataa tattgctaaa
     2401 ttttttacgg attctcacaa tattcaatta gattgggaaa aaaattatcc ttatgataaa
     2461 atttcaaatt ggattttaga gtttaaaaaa gatactacaa gctattggaa tgttttagaa
     2521 tataaagtag atgttgatga aaaaacctac ataattacta tggccaatga tataacaaaa
     2581 ctctatgaag aaaataagag aaatcttcac tactcaatta ctgacccatt aactggagca
     2641 tataatagaa aatatttaaa tgatagattt gacatattta taggagatta tattgtacta
     2701 attgacttag ataatttcaa aatgataaat gactatgaag gtcataatgt aggagacaag
     2761 attttatgtg attttgtttc acttttaaat aaagaattaa taaattctac ttctattata
     2821 agattaggag gagatgaatt tattgttata ttctctagtg atgttgacaa aaattatgtt
     2881 tattcacagc tagaagcctt aagagaaaat tttttaaaag tattctctaa gtataagtat
     2941 ctttcattta gttatggtgt tgatacagta aaaagaaatt taaaattgac aatagttgaa
     3001 ttggataaaa aaatgtataa aaacaaggag aaaaacaaga aaaaatttga taaaaatgat
     3061 tattaatatt gttattttaa aataaattaa aattatcttt ctttttttaa acttgtacat
     3121 tagtttataa atatgtatct atacaagata ttgacattaa gtactgaagc atatattgtt
     3181 tcagttcttt tttgttaaaa ttaacttatt tgacaaagat atcatagttt aacaaagctt
     3241 ttaatttaaa tatattgatg tataatataa ttaaaatata atagcaaaaa gaaaggagga
     3301 atatcatgag catactccta aaaaaagcac ccaaattagc aaaacatatt ataacaagtt
     3361 tttatataaa tcgggatatt gatgaagtat taaaatacct ttgtgaaaat gttacatgga
     3421 ttggaccagg agaacaggaa tttctcactt cttttaatga aataaaaaat tatttttatg
     3481 caggtcaaga tgaaattcct tcttgtgata taaataatga tatatttgaa actgtaagtg
     3541 aagatgagaa tagatgtatg gtattaggaa gatatactgt tcgaactaaa gaaaatgctc
     3601 aaatgatttt agaggtaaat caacgttgta catttgagat aattgaggat agaggaaaat
     3661 tattaataaa gcacatgcac atatcaaacc cttatggtga aatgcaactt gatgaatact
     3721 ttcccactaa aattggaact caaagttatg attatttgca aagattacta aaagaaaaaa
     3781 cagaagttat tgaaatgatt acaaataata tcaatggtgg cttaaaaggt agcaatgatg
     3841 atagtacata ctcatttttc tatgtcaatg agggattgcc caaaatatta ggatacacat
     3901 atgatgagtt catggaaatg agtggtggtt cagcagtggg tgctgtatat ccaccagact
     3961 tacctaaagc actagaagat tgtgaacgat gttttgcaaa aggacctaca tattcatctg
     4021 aatatagaat ccgaaaaaaa gatggaacat taatgtgggt attagattct ggaatgaaat
     4081 cgttaaatag tgatggcata gtaaaaatta atagtattat tactgacata acccaactaa
     4141 aaaacataga atctgaacta aaattagaaa gagagcgata tcgtattgct ttgcaaaata
     4201 ttacagatat catgtttgag tatgatattg aaaacgatac ccttataaaa tatcaacgtg
     4261 tagaaattga caaaaaaatt gaattagaaa attttgaaac aaaaaattac tccaaactat
     4321 tagaatctgg taaaattatt caccttgatg atattggaaa actactagaa gtacttcatg
     4381 gaaatcttcg tgaaacaatt gagataagac aagtaaattc attaactaaa aatgaatgga
     4441 gatggattcg tgttcaatgt tcagttatat atgatagtga ccataaccct atcaaaacaa
     4501 taggcgtttt aaaagacata actgaagata aatctaaatt agaaagctta attaatcagg
     4561 ctcaacgtga cccactaaca caattatata atcaaagagt atcacaaaat cttattgaag
     4621 agtatctatg tagttctgat tccaaaaata atgatgctct attagttatt gatattgatg
     4681 attttaaaac tgtaaatgat acttttgggc atttagaggg aaatgaagtt ttagttgctg
     4741 tatcaaaaat actattacat aacacacatg ataaagacat agttgctcga attggtggag
     4801 atgaatttac aatatttata aaatcattaa caaaggattt aataataaaa attactaatg
     4861 atattttaaa tgatgcaagt aaaataaaag taaaggacaa tcataaaata acattaagta
     4921 ttggtattgc cttcactgat gattcaacta aattatataa agatttattt tctaaagctg
     4981 ataaagctct ttatctatca aaagcagatg gcaaaaattg ttatagtaca tacgaataat
     5041 aaagctattt atacgaccaa gaatgaacct ttaaatttaa tttcatagtc taacaatcaa
     5101 agaattgaag ctaatcttat agctagtcaa ctagttccta gaaaaaattt agacaatata
     5161 tttattgata ctgctcattt ttctaaagag caaatttata taaaaaatta aacttttatg
     5221 tacatacaga tactaatgat aaacatttag cattagtatc ttctatctta aaacaaaatg
     5281 ataatttatc tatataaaaa agtgctttgt tgacagagtt cactttttta tataggtaca
     5341 gaatttaagt ataaatacat gttttattct atacatttta gttaatgtaa aattccttga
     5401 ttgtactctc taacttttca acattatact atttacatac cacttagtta atataaaact
     5461 ataaaagtgt tgcaattact gggttttata aagttaattt acataccact tagttaatat
     5521 aaaactttag caccttattt atctatagag ctacagaaag tatttacata ccacttagtt
     5581 aatataaaac aagcatcaaa tacatttatc attctgtcta taattttatt tacataccac
     5641 ttagttaata taaaactaat acctcctttt ataaaaaaat gtcgtgaaaa agtatttaca
     5701 taccacttag ttaatataaa acatgtattt atttgttctt gtactattgt tttactctat
     5761 ttacatacca cttagttaat ataaaacact ccaaaacttg attctaaatt tagaaaagga
     5821 ctatatttac ataccactta gttaatataa aactgtagaa agctcgtttg tggatgaagt
     5881 tctaaaatca tttacatacc acttagttaa tataaaacgg aacttttaaa agattcaagg
     5941 aaaaaagatt ggtttattta cataccactt agttaatata aaacttgtaa ggataagcct
     6001 agagagaata gcccttctct ctatttacat accacttagt taatataaaa cattattttt
     6061 ttatcgtttt gaaacctgtt tcttctgcat ttacatacca cttagttaat ataaaactat
     6121 cttttgatta ttctgataaa aagtaataat acaaatttac ataccactta gttaatataa
     6181 aacgctcccg aagttgttaa tgttgttgcg actcttgctg atttacatac cacttagtta
     6241 atataaaaca tcaatacttt gatgcagttt ttaactagat tttcaattta cataccactt
     6301 agttaatata aaaccccaaa ataaactggg cattttcaat acttacacat atgcaactct
     6361 cttaaatttg cagtgaacca tgagtagtgc aaatgataaa gcttatcata taccctcaac
     6421 accttatatt tcaagcgtta agctctactt tgtaacaaat atcgctcact gcaaaactat
     6481 ttacattttt attatatcat aaatatatta tttttgaata tctataccaa tttgtggtat
     6541 aataaaagca agaagaacta caatctattt agacgtagag tgaagttcaa atactaatgt
     6601 ttattctttt tgaacttgat ttttagttca aaactaaagt cactcctgcc tgtgtgaact
     6661 atacccgctg ttttatacag ttaaaacatg taaaaatagt tataaagtac tagtgtaact
     6721 cattagtact ctttttatta tattcatata attaatatct atattttaaa gtttactatc
     6781 taaaaaagag cacgtcaaat aaaaccctag atatttaatt tttttagaga tacttaaaat
     6841 aaattatgct ctctctgtac ttaaatattt cctaaattca aatggagaca tagaatttaa
     6901 tctattttga tatctaaaat tattatagta gtatatataa tcatctattg ctttaactaa
     6961 ttattcatat gtatcaaatt tccttaaata gtacatttct acctttaaaa ttccccaaaa
     7021 tccttccata ggagcattgt caaggcaacg accaactctt gtataatata tgtatatcta
     7081 acaccattaa gaaatatata ttttataata tatatttctt catttaatgc acttagaaaa
     7141 gcaaagaaaa tacagattag tacacttttt agagaaaaat aaaattgata catctaaaat
     7201 attaaaaaat actttagata atctaaagac acatctcaaa gtttttaact aaattataaa
     7261 actaattaca aatgctatgt aacgataata aataaaaaat ttacttcgct taagcgacgt
     7321 gtcgttgaaa tatttcatgt tgccaactgg ttcgtaagtt gataagaaaa gaacactaat
     7381 tatttagagt tcttttctta gctacaagtg gattataata cttttaaatc tgtgcgaaaa
     7441 ttgtatataa taatgatatg aacaagtaaa gaaaatacaa aaatgacaaa attataaata
     7501 aaatattata aaatcataag gtcataattg ttatattgta gaaacaaagt aaagagagat
     7561 ggtggagaat aaatgaatca aaaaaaaata aagattagta ttaaaatggt aattataggt
     7621 atatttgctg taattatttc ttttgtatta tccagattat tcgttgagta tattgatgaa
     7681 ttattaaggt ctaattggca atatcagcta tttgaaagtg agagttcata tataatattt
     7741 gccaattgta ttatgttctc gaatatatta ttagaaatat ttttaatata tatttgtaga
     7801 aaatttagaa aaatctaata tagtcaacgt atttcgaatc gaatacttca tgattattta
     7861 gtctattgtg ctagttaaag tttaattccc atataaagta aattgatttt agtaaaacaa
     7921 atcaccccta agggttagtt tatggataag tcatccaaat attaactctt taatttttgc
     7981 tatagattca tctttgctca taactttact tataaaaaat gaaatattat aagctagaac
     8041 ttttattcta attctagtca taaatcctag catagagtta ctttttttat attataaata
     8101 aaactataca acaaagataa tatttagtaa aaataacttt cattttaact aaacttaaaa
     8161 ttcaattact ctctaaattg acgtttagga attagttcaa aataaaaaag agtgtatata
     8221 ttgaaattac tacatttcaa tatatacact ctttttaaat ttctcagttt tccatggaac
     8281 tttactttat ttatttctgt attagttgta ttttttatgc atttttcttt aaaatcatat
     8341 tttctgattc aactcttata ttataaaatt tctttactaa attattctct ttttctatat
     8401 cttcatatat attttttaat cgccttttac ctaatctttt atctaacatt actaatgatt
     8461 taactaatat attatatgat tttatcgcat cttctactga gatatttaaa catatattta
     8521 ctgctttata aaaatcaaat tcactaaaaa atccattttt atttacaatc ttatatccct
     8581 ttgcatctct ttctaaatac tcttcctaaa tactcttcaa aactttcatc atctaatcta
     8641 aatcctatat cactatgaaa aataggtttt gcatttggat atagttctat tgctttatca
     8701 aaagtttcga ataccaagtt attattattc gattttccaa tagcccacta taaacaataa
     8761 taagtttgtt gctccaaata atgtaacact tgtagaaaga tgttacagat atataaaaga
     8821 tagagaatgt agtttttctc ctaatactat tagaaataga aaaaatatga ttaaaaatca
     8881 aattgaagtt ttctttaaag atatgaaatt aatagatata acaccttcta ttttacagtc
     8941 ttatataaat aacatataca atgagcacat gttaaattca acaaaaaacc aagtcgattt
     9001 tataaaatta gtattgaaag aatcttatag attaaaagaa atatcagaaa atatttgtga
     9061 ttttgttaca accccaatca aaaaaatagt ttaacttcta agctttatac aaaacaaaaa
     9121 gctcaattat tattagaaaa atcaaaaaat actcctatag gtataccaat ttttttgatg
     9181 ctaactcttg gattacgttt tggtgaagct gtatcactta tatggtcaga tgtagacctt
     9241 gataaaaaat ctcacatgta aatcaaacat tagtttatgt atacaataaa ataatcttca
     9301 aaggccctaa aacccctaga ttcaaaagaa gattatttgt tcctgatgaa cttataagtt
     9361 tgcttaaagg ggaaaaatta aaacaaaata agttgaaatt acagggtata ttgaaaaatg
     9421 aatttgattt gatatgctta gataaaagat tcaacccgtg agttcaacca acttttgttg
     9481 ttcattttaa aaaactatta agagataata atcctagaga tataaaagta catgaattaa
     9541 tacatgcaaa tgcaactttg atattacttt caggtactaa cataaaagct atttataaaa
     9601 tattagtaca tgcagatata aaaatagcta tgaataaata ttcgatattc tcacttatta
     9661 gatgaaatgg ataaagaaac atctgaaaat ataagcaaaa tactattcaa ataatgttca
     9721 gttttatgtc aattaaatgt cactcaaagt gctttttttc catttgtcag ttaaatgtca
     9781 cttaaagtac ctttttccta tttgtcagtt aaatgtcagt aatatataaa taagatatat
     9841 ttataagacc ct
//
LOCUS       DAKBNN010000146         9754 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000146 DAKBNN010000000
VERSION     DAKBNN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.147"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            208..1041
                     /locus_tag="OUB02_002852"
     CDS             208..1041
                     /locus_tag="OUB02_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HCT8886591.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1134..2066
                     /locus_tag="OUB02_002853"
     CDS             1134..2066
                     /locus_tag="OUB02_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT8886592.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2118..2600
                     /locus_tag="OUB02_002854"
     CDS             2118..2600
                     /locus_tag="OUB02_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT8886593.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2612..3481
                     /locus_tag="OUB02_002855"
     CDS             2612..3481
                     /locus_tag="OUB02_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCT8886594.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3524..4279
                     /locus_tag="OUB02_002856"
     CDS             3524..4279
                     /locus_tag="OUB02_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCT8886595.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4321..5142
                     /locus_tag="OUB02_002857"
     CDS             4321..5142
                     /locus_tag="OUB02_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HCT8886596.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSGT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5147..5908
                     /locus_tag="OUB02_002858"
     CDS             5147..5908
                     /locus_tag="OUB02_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCT8886597.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6069..6326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6439..6678
                     /locus_tag="OUB02_002859"
     CDS             6439..6678
                     /locus_tag="OUB02_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCT8886598.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6982..7977
                     /gene="ilvC"
                     /locus_tag="OUB02_002860"
     CDS             6982..7977
                     /gene="ilvC"
                     /locus_tag="OUB02_002860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCT8886599.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8030..9718
                     /gene="ilvB"
                     /locus_tag="OUB02_002861"
     CDS             8030..9718
                     /gene="ilvB"
                     /locus_tag="OUB02_002861"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCT8886600.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVASMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
BASE COUNT         4173 a          940 c         1754 g         2887 t
ORIGIN      
        1 aaaaacacta tataatactc aattttcaac attctataca attatacaga aattatacag
       61 cataataatg accgaaataa caaattttaa aaattaagca aaactttttg tcgaagttca
      121 acgtctatat atatgtagtg taaatttttg ggataaaata ctacaaaaat ataaatgtaa
      181 aaatatagta atattggagg tcgaacgatg agaaataaaa agaaaaaaat aaatagaaag
      241 aagttgtatt tgcttttaag tgctattgta gtgtgtctgg cttttatagt agttggtgtt
      301 cgcgtcagct ctttaaatat gaaggaaaat gaaattagta gaaatgcaaa actgtcaagt
      361 tctactatta cagatatagt atctaataca tcaatagaag ttagtaaagg gccaagtaaa
      421 agttctggaa aaatagcata cataactata gatgatggtc catctaaatt tactgacgag
      481 atgataaaaa ctttaaataa atacaatgtt aaagcaactt tttttatgat agatgggaat
      541 atgaaggaat atccacagca agttaaaaat ataattaaaa atgggaacac agcaggtttt
      601 catagtgtat cacatgatat tcacaagctt tatgttacta gtacatcagc aaaagaggaa
      661 tttgatacaa atgatcaaac tttttataag ataactggta aacattcaaa agtaataaga
      721 ataccttatg gaagcaaacc atatacacca caagcctctt atcaggcttt agttgatgct
      781 ggatataaaa tttgggactg ggacttagat acagaagact ggagatctaa ttcttctcaa
      841 attgtacaaa atgtaaaaaa tcatattaag aatagaaaag gtgaagataa agaccaactt
      901 gtagttttaa tgcatgagaa aaaacagagt gcagaagctt tagattcagt tttaaaattc
      961 ttatcagatg aaggttatga atttgcgcca gtagatcaaa accaaatacc aaaaaattat
     1021 tggttacgta atttagagta gttaaaatat aagaatattg attgatattt ttagaaattg
     1081 agaataaaaa tgagtgaggt gtaggtatat tgaataaaaa aatttatata ggaatggtcg
     1141 gaatattgtc tttgatgatg gttggttgta ataaaagttt agcaaaagta aatgatgtgg
     1201 aaatcacaaa agaacagtac aaaaaaacaa aagcagtttt atctgctact aataattata
     1261 taaatgggca gtctttagat gaattagaaa aaactttgga taaaaaaggt agaaataaat
     1321 tagaaaatgt tataatatcg tttatggtgg ataatgaact tttatatcaa gaggctaagg
     1381 ataaaggact aactccaagt aaaagtgaag tagactcaaa atatcaagaa ctagaagata
     1441 agatgaattt gaatacaagc tataaggaaa aaatggacaa agcaggtgtt gataaagatt
     1501 atttaaaaca agaaatatct agagatttag caatagataa aaataaaaag gcctttgaag
     1561 atagaataaa cataagtgat aatgatatgg aagcctacta tacaagtcat aaaaaagatt
     1621 ttaatgtaga agaagtaagt gcttctcaga tacttatatc gactttagat aaaaataaaa
     1681 aagaagtaag caaagataaa aaagaagctt tgaagaagaa ggcagataat atattgacta
     1741 aaatcaaaaa tggagaaagt tttgaaagtt tggctaaaaa atattcggat gataaagcaa
     1801 ctggaaaaaa tggaggacag ctaggatatt ttacaaagga tgataaaaat gctgagttta
     1861 caaaggaagt gttcaaatta aagaaaaatg aagtttcaaa tgtgtttgaa actagttatg
     1921 gatatcatat agtcaaagtt actgataaaa gagaaagaca aaaaagtttt aatgagtgtc
     1981 agagtttaat aagagaatct attttaaatg aaaagtatat tgagcatata aaaaaactga
     2041 atgaagatgc taaaatagac agatagaatt tctaacagct caatcctagc ctgtaatcgg
     2101 agggagattt tacaggaatg tttaagagtt taaagagaac gaaagttgag aaatacatta
     2161 tcgataataa agactctttt tatagaatag catatagtta tacgaagaat gaagaagatg
     2221 ccttagatgt ggttcaagaa gccatgtata aggcactata ctcagtggaa aatattaaag
     2281 aagtaaatta tataaagaca tggttttata aaattttagt tagaacgagt atagatttta
     2341 ttagaaaaaa ccgtaaatat aataatatga cagatattga ccttatagat gagactggag
     2401 agtatgataa atatactgat ttggatttaa gaagagcttt ggaagaatta cctattgaat
     2461 acaaatctat tataatatta agattttttg aagatttaaa gattgaagaa gtagcaataa
     2521 tattggatga aaatgtgaat actgttaaaa ctaggttata tactgcttta aaaaaactaa
     2581 aattaaaaat tgaagagtag ggataaaaag aatgacaagc aaagaaaggt taaataagtt
     2641 aaaagatgag tatcataaaa ttccaatacc caagaaatta gatactataa taaatcatga
     2701 aaaaatagaa aacatatatc gagagaaaaa taaaaaagta aatagattaa gatttaaagt
     2761 tgcaatagcc tttgcatgta tatttacagt attagtaaat ataagtcctg tatttgcaga
     2821 caatttttct aagatacctg taattggagc aatagtagaa gtaataacta taaaaaatta
     2881 tagtttaaaa agtgaaaatt atgaagctga gattgatatt cctaaaatac gaggtttaaa
     2941 agataaaaac ttagaacaaa gattaaacag tagttttatg gaagatggaa aaaggcttta
     3001 tcatcaattc caagagagaa tggagaagat acaatcatca aagaataaag gatataaatc
     3061 tttaagtttg agttatagtg taaaaaataa ttctaaaaaa tttctatcaa tagagatgac
     3121 aaaaaatgag atagaggctt catcatatgt gagtaaggta cattatacta ttgataaaaa
     3181 aagacaaata gttttgactt tgccaatgct ttttaaagat gataagtata taaaagttat
     3241 aagtgataat ataaaagaac aaatgagaga acaaatgaaa aaggatagta caaaatccta
     3301 ttttatagat caaaaaaaag acttgcctgt tgaagatttt aaaacaatta ataagtatca
     3361 ggatttttat tttaataaaa atgaagacct tgtaatttgt tttgatgaat atgaagtagc
     3421 acctggttat atgggcgcag ttgagtttgt aataccatat aaagtaataa aagatttgta
     3481 aattgaatag tagtttatat ataaatcttt taaggaggac aatatgacta catataaaaa
     3541 acttaaaaaa tctatattta aaattgctat attttcaata tgcttattag tttcattagt
     3601 agatacaaac cctatatttg caggtagttc ctttaatgaa acttcaaata gtgatacgat
     3661 aaaggtgtta aatgctaaga gtatcaactt taaacaagac aattatgaag tgaatgtgag
     3721 tattcctaaa ataaaaggtt taaaagataa aactttagag aataaattaa acaatgagct
     3781 tttgcaagat gggaaaaaaa tttacagtga atttcaaaat aaaatgaaaa aggaaaataa
     3841 taagggtcat agatacctaa atgtaatcca taatgtcaaa aataattcta aggacttttt
     3901 atcaatagaa atgacaaaag aagagataga agcttcctca tctataacta agaagcatta
     3961 cactgttgat aaaaatagag gaatactatt aaatttacca atatttttta aagatagtag
     4021 atatattaga gctataagtg ataatataaa agagcagatg agaaatcaaa ttaagaaaga
     4081 tgatacaaaa tcttatttta tagaccaaga taaagactcg ccagtgaaag attttgaatc
     4141 tataaataag tatcaagatt tttattttaa taaaaatgga aatcttgtga ttagttttga
     4201 tgaatatgaa gtagctcctg gttatatggg agcagtagaa tttgtgatac caaataaagt
     4261 aataaaaaga ctaagataga tatatagaag atttttagat agactggtaa ggagaattat
     4321 atgaatatat atgaacaagc aataagatta atagaaaata atgaggattt cgcatttgct
     4381 acaataacgt ctcattcagg ttcaacacca cgtgaaactg gagcaatgat gattgtgaaa
     4441 aatgattcta ctatatttgg ctcagtagga ggaggtagtg tagaagcaga atgtataaaa
     4501 catgcaatta atgttataaa aaacagagaa tcaatgttgt ataaatttac tctaaacaaa
     4561 tctgatgtgg caaaactagg aatgatatgt ggaggtactg gagaaataca aatagatttt
     4621 atagatagta aattaaaaag taatatagaa aaatttaata agagactaaa ggaaaataca
     4681 agtaaagcat atatatttgg tgcaggtcat atttcaagag atgttgctgt gatactatct
     4741 cttttagaat ttagaacagt agtaatagat gatagagaag aatttgctaa tcatgaaagg
     4801 tttccagaat cagaagtagt agttttagat tcttttgaaa acataccaga ttttccaact
     4861 gacgaaaata gttatataat tattttaaca agaggtcatc tatatgattc aagtgcttta
     4921 gaatgggctc ttaaaagaga ggctggttat ataggtatga ttggaagtgg aacaaaaatt
     4981 ggattgactt acgaaaaact catgaaaaaa ggttttaaga aggaagaatt atcaaaagta
     5041 catgcaccaa taggcataaa attgaatgct caaacaccag ctgaaatagc tgtatgtata
     5101 gctgctgaac ttataaattg tagagctaat aaggaaaaat aaactaatga acacagggag
     5161 taaagatatt gatactttaa aggttaattt tagagggatt gaaataaaag cagttttagt
     5221 atataaaaat agaaaaaata tttctataaa aatagatcca tttggcaaaa taactgtaat
     5281 gagtcctcct aaaatatcca aaaaaataat aaaagatata attattgaaa aaggagactg
     5341 gatacttaaa aagtcagaaa aatgtaaagg aagagaggaa gtatataaac aaagaatgtt
     5401 tataactggc gaaaaatttc tttatttagg tgaagagtac catctagtta taaaagaact
     5461 gttgagagat tctgtcaaaa aaagtaactg tagaataact attaacgaat atcaaatagt
     5521 tgttcagaca aatgatacaa gtacagattt tattaaaaaa tctttaaaat cttggtataa
     5581 gatggaaagt gaaagaatag tattagagag aatagatttt cttaaattaa attgtgaaat
     5641 aatgaagcaa ttagttccag cgagtgttaa ggtaaaagaa caaaataaga gatggggaag
     5701 ttgtacagct caaaaaaata tttatataaa ctcaaaaata tctatggcaa gattagatgt
     5761 aattgattat gtaataatac atgagtttag ccatttagta catatgaatc actctaaaaa
     5821 attctatgat ttagtaaaat ccattatgcc cgattataaa gataaagaaa attggctaaa
     5881 aataaatgga tataaaataa taatctaaaa acgattattc caaaatatag gcgtaaagta
     5941 taaaagaagt attattgtga aaaaaataaa taatctgaaa attttaaaaa aagtcattga
     6001 cattgtaaat gatagggaat ataatctata ttaacaacaa aacagaatta aaatataaaa
     6061 ttaaaaaatt aaaagcaatg acagagacaa caatactaat agtgttatcc acagagaacc
     6121 aatgttgctg agaattggtg ttaacagtag tataatgatc actctggagc ttttgtactg
     6181 aatattttta gtaagtacaa acgtatgtca gcgttacatg actaaggtta aggtaattaa
     6241 ttacccgacc tgaaaaaaga ggggtagatg tattttttat atctacaaat aaggtggtac
     6301 cgcggaatat aactttcgtc cttataagta atatttatat tgcttataaa gatgaaagtt
     6361 tttttatttt aagcgatata aataaattta atccatataa aattaataaa aaaataataa
     6421 aaataaggag ggatttttat ggaaaatact gtatatgcta aattaaacca aggaatagat
     6481 tcttttttaa gagtggctat gactctaaga agaagagagg ttgatataca atcaataagt
     6541 atgactgtag ataatatgaa taattcaggt ataaaattaa tagtaaatga agaaaagaca
     6601 tctttagatt ctgtcttaaa ccatatgaaa aaactacatg atataagaga gataactgtt
     6661 gaaagaatga gacattaatt aaatatattt tataaaacta aagataaata tgcttatttt
     6721 aaaataaaaa actaaagata aatattaaat aagaaaaatc ttagattaaa atataagtgt
     6781 cattgaattt attagtttat cacagttaat aaatttatta gtttgttgta gctaataaaa
     6841 actgaaaatt ctaaaattat aattgtaaga ttatataaat ttagaattta aatctgttga
     6901 ataatattaa aacaatgata atgagtatga atttctaata aaaataaata tcataaaaat
     6961 aagaaaaagt attgggagga aatgaaaatg gcaagaatgt attatgaaaa agatgtggat
     7021 ttagaggttt taaaaaataa aaaggttgca gttttaggtt atggaagtca aggacatgca
     7081 catgcacaaa atcttagaga taacggtgta catgtaatga taggtcttta tgacggaagt
     7141 aaatcagcac aaaaagcaaa agaagatgga tttgaagtta agagtgtagc agaagctaca
     7201 aaagaaagtg atttgacaat gatgttaatg cctgatgaaa aacaaaagaa agtttatgaa
     7261 gagagtgtaa aggataactt aaaagaaggt cagacattag cttttgctca tggattcaat
     7321 atacattata atcaagtaca accaccagaa tttgttgatg ttgtaatggt agctcctaaa
     7381 ggacctggtc acttagttag aaatgtattt acaaagggaa gtggagttcc tgcactattt
     7441 gcagtatatc aagactacac taaaaaagct acagagacag ttttagcata tgcaaaaggg
     7501 ataggagcaa caagagcagg tgttttagaa actactttta aagaagaaac agaaacagat
     7561 ttatttggag aacaatcagt actttgtggt ggaataagtg aacttataaa attgggatat
     7621 aaaacgcttg tagatgcagg ttatcaaaaa gaagtagcat attttgaatg tttacatgaa
     7681 atgaaactaa tagtcgacct tatatatgaa ggtggatttg aaagaatgag atatagtata
     7741 agtgacacag cagaatatgg agattatgta tctggtaaaa gagtaataac tgatgctgca
     7801 aaacaaggta tgcaaaatgt tttagaggat atacaaaatg gaaaatttgc aaaagcgtgg
     7861 attaaagaaa atgaagaagg acgtgaaaac ttcctaaaaa ctagagaaga agaatataat
     7921 acagaaattg ccgaagttgg tagaaatcta agaagtatga tgtcattctt aaaataaata
     7981 tatattaaat ggattagttt gagggaaaga gtggtgtata gtagtgcgta tgaatggagc
     8041 aaaggtaatt ttagaatgtt taaagaaaga gggaatagac acaatatttg gatatcctgg
     8101 tggagccgtg atacctcttt atgatgctct atacgattat tcagatgatt ttaaacatat
     8161 aaggacatcc catgaacaag gtttagttca tgctgcagat gggtacgcaa gaagtactaa
     8221 tacagtagga gtttgtttta caacatctgg tccaggagca acaaatgcca taactggaat
     8281 agcaacagct tttatggatt cttcacctat ggttgtaatt tcagggcagg tgccaactag
     8341 tttattagga aaagattctt ttcaagaaat tgacataact ggagcaacat tatctatgac
     8401 aaaacataat tatttagtta gaaacactaa agaattagtt cctacaataa aagaagcttt
     8461 tagagttgca aattcaggta gaaaaggacc tgtacttgta gatgttccaa aagatttatt
     8521 tttagcagaa atggatttta gtggtgagga ctatgattta tgtcaaatag atgattatat
     8581 ggattataag agtgattttg atctagatga tgaaactaat attaagcttt taaatgaagc
     8641 aatagatatc ataaaagaat ctaaaaagcc tgtaatatat gcaggtggag gtgttaaatc
     8701 ttcagacagt gaggaaattc ttgaaaaatt tgcaactaag atagatacac ccgtactaaa
     8761 tacacttatg gggcttggaa atatagatag aaagaatgaa ctatctcttg gaatggttgg
     8821 aatgcatgga agtagagaaa gtaatttagc actttctaat tcagatttag tgatagcgat
     8881 aggtgcgaga tttagtgata gagtgataag taaaagttct gaatttgcta aaaatgcaaa
     8941 aataatacac atagacatag acccatctga aataagtaaa aatatagaat ctaatgtatc
     9001 tttggttgga gatgtaaagc tagtccttag tttattaatt gaaagggttg aaagtaagaa
     9061 taatagcaat tggaaagaag agataaagac atttagaaag agtgagggtg tacaaacata
     9121 tgaatttcat ccacaaaata tacttaaaaa aataaatgaa aaatatgaaa cattaaagaa
     9181 acccactgtg gtagtaactg atgtcggtca acatcaaatg tgggctgcta agtactggaa
     9241 ttttaaaggt aataaaagtt ttataacatc agcagggtta ggaactatgg gatttggttt
     9301 aggtgcagct attggaacta aagttggtaa tgtagataaa aatgtagttt tagtcactgg
     9361 tgatggaagt tttagaatga actgtaatga actagctaca gttgcaaatt ataatgtacc
     9421 tatgcttatt ttactactca ataatagaac attaggaatg gtaaggcaat ggcaaaagtt
     9481 attttccaat caaagatact ctcaaactga cattaatgaa aatgtggatt atgttaaact
     9541 cgtaaatgca tataatattg atggatataa ggtagccagt atggaagaat taggaaaggc
     9601 gctagatatg atagatttta ataaacctgt atttttgcaa tgtgatatag ataaggatta
     9661 tgatgtttat cctattgtag cacctaatga tgcattggaa aatttgatat gtaactaaaa
     9721 cttatactga atattgataa aattttttat taaa
//
LOCUS       DAKBNN010000147         9446 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000147 DAKBNN010000000
VERSION     DAKBNN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.148"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            40..888
                     /locus_tag="OUB02_002862"
     CDS             40..888
                     /locus_tag="OUB02_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8886601.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            878..2542
                     /locus_tag="OUB02_002863"
     CDS             878..2542
                     /locus_tag="OUB02_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8886602.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            2591..3847
                     /locus_tag="OUB02_002864"
     CDS             2591..3847
                     /locus_tag="OUB02_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8886603.1"
                     /translation="MRDLNVSEYLFIFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            3870..5951
                     /locus_tag="OUB02_002865"
     CDS             3870..5951
                     /locus_tag="OUB02_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCT8886604.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            5988..7073
                     /locus_tag="OUB02_002866"
     CDS             5988..7073
                     /locus_tag="OUB02_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCT8886605.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            7127..8251
                     /gene="wecB"
                     /locus_tag="OUB02_002867"
     CDS             7127..8251
                     /gene="wecB"
                     /locus_tag="OUB02_002867"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCT8886606.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            8329..9303
                     /locus_tag="OUB02_002868"
     CDS             8329..9303
                     /locus_tag="OUB02_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCT8886607.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         3961 a          862 c         1533 g         3090 t
ORIGIN      
        1 caacttaata gaataattgg aggtgtaaat ttataaaaaa tgaataggat aaataaaaaa
       61 atagatttat taatattggc tttgctcgtt tttatatttt taattgttgg aatagtaata
      121 ctatcgatta ggacagaaaa taaattgaat gtgttcataa tgttagcaat aatttttttt
      181 ataataatgt taacttattt atcaaattca gtagtggggt tgataacaag ttcaataatt
      241 atatttatgt atacatccta tattttatat aacaatatta ctcacaatat ggatgttgaa
      301 tttatatcat atatgtggat aatagcaaca ccagtatctt caattataat gggtaatctg
      361 aacaaaagca taaatgaatt gcaaaataca aataaaaaac tatctgaaca gtataaggaa
      421 ttagtgacta ttgatagtga aactggactt agaaatttaa agatttttta taatgatgta
      481 aatatggaga taagtaaatc tattagacat aatactgatt tttctcttat gatagtaaaa
      541 ttaccatact acggaaacct acagactata tttggtgaaa acaaaactaa taaaatagta
      601 aaatatattg gttcaaatat tatagaatgt acaagaaatg aagatataat ttatagttta
      661 caaaaagata tgatagggat actaatgcca aatacatctt tggaaggttc aaaagttgtt
      721 aaagacagaa taaagaaaag gataaaagaa ttaaacttag atttaaacaa taggggaaaa
      781 tatgtaaata tagatgtcaa aattgcattt ttacaatata agagtagttt tggagatagt
      841 ataaatttta agaatatagt tgaagaggaa cttcaatatg atgtttaaaa tatttaataa
      901 aagtctatta aaattagtag ttgtattatc aatagtactt acaatatcaa attttagctt
      961 atcttcatac gcatcagaaa ttacttccag cacagataag gtattaatca tatatgattc
     1021 taagaaagaa acagcttata atagagatat tttaaatatt atgaggactc tattgggaag
     1081 atttagttca gatatagagc tattaaaatt gagtaactat gatggtgaga taaataaaaa
     1141 ttattacagt catatattta ttttaggaat caatgaaaac tcatataata atgataaaaa
     1201 cactaaaaac ttgattagtt ccttaaattc atataaagga actatatgtt ggttaggata
     1261 tggtatagaa aatcttttgg aacataaaaa atacaattta gactatgtag gaaaaacaaa
     1321 caatatagtt agtgtaaatt atagaggtaa atcatataac ttggatgaac attatgtttt
     1381 taacattgta gaatcaaaag atacaagtaa taaagtaata ggaagtatta atgatacctt
     1441 aaataagtat ccatatataa taaatgataa aaatcttttt tatgtatcaa aattagattt
     1501 agatggagtt ttattttata tattttgcga ttcacttaat gatattttta acattaagac
     1561 gtttgataaa ggacgtatat ttgtaagaat agaagatgta catgctttta gagaaccaaa
     1621 aaatcttgta gagatagcgg attatttaag tagtaagaat ataccattta cgattgcatt
     1681 aataccagca tatgtaaatc ctaaaaatca taaagtaata actttatcag aaagccctga
     1741 aattgtcaaa gcaataaaat atatgcaaga taaaggtgga actgtgatat tgcatggata
     1801 tactcatcaa tacaagaaag aagaagtttc tggagaaggg tatgaatttt gggatggtaa
     1861 aaaagatgaa cccttaaaag aaaatatgaa gatatttgtc aaagatagag ttttgaatgg
     1921 tcttagagta tgtatagaga atggaattta tcctcttgca tttgaagcgc cacattatgc
     1981 aatggaatca gagggatata aagaattaaa aaaatatttt tctacttata tggggcaaca
     2041 tcaaaataat gataaaaaat ttagcacaaa tacatatcct tatattatta gggatacaga
     2101 ggagtttaat atatttatac ccgaaaattt aggatatata gacccagaag ataagtttac
     2161 ttttcaaaat ataaaagaaa atttagataa attatctatt gtaagaggtt ttagtggagg
     2221 gttcttcttt cattcatatt taaacataga gtatctaaaa aacacaatag aatatttaga
     2281 aaaacagaat atagaattta tgaatttaag agattttaat aattgggtaa aagttgatga
     2341 aatacaaata agaaataatg gagatgaaat tatagtcaac tatgataagg atttagatga
     2401 aataactaaa agtgatacta gatttaaatc catatccaat attagtaaaa ttttaatatt
     2461 catagtatct ataagtgttt taatatttgt gattatattt atatatttta agagaataga
     2521 taaaaagaaa tttttaaaat aaatgatttt atattagaat caatatttta aaatatttta
     2581 ggagaaattt atgagagatt taaatgtatc agagtatttg ttcatattta gtctgtttag
     2641 tatatggtct ttattgctta taaatattat tttagctatg ggtggatata tattttattt
     2701 taaaaacttt gataaagaaa taaaagaaat tgatgaatat cctatgatat caatacttgt
     2761 tcctgctcat aatgaagcta aagtgatagg tagaactgtg gagtctttgc tattactcaa
     2821 ttatcctaag agtaagatgg aacttattgt tataaatgac aattcttctg ataatagtaa
     2881 agaaatttta gaaaatataa aggataggta caacaattat aatttcacta taataaatac
     2941 agatagttta acaggtggaa aaggaaagtc taatgcatta aatattggat atacgatttc
     3001 aaaaggagat tttatagctg tttatgatgc agataacaca ccagataaaa atgcacttag
     3061 atatttagta caaactatag taatgaatga tgaattaggt gctgttattg ggaaatttag
     3121 gactcgaaat aaaaataaaa acctattaac caaatttata aacatagaaa cattaagctt
     3181 tcaatggatg tcacaagctg gtagatggca actttttaat ctatgtacaa taccaggaac
     3241 taactttata ttgaggagaa gtatcataga agaaattgga ggatgggata gtaaagcaat
     3301 tgcagaagat acagaaataa gttttaggat atataaattg ggatacaaga taaagcttgt
     3361 accacaatct ataacatggg aacaagagcc agaaactgtt aaggtgtgga taaagcaacg
     3421 tactagatgg gcaaaaggga atatatatgt attaatgaaa tatataaaaa atatatttaa
     3481 acagggaaga aataagattg tgtttgatat agcttacttt ttttctgtat attttctatt
     3541 tttaacatct gtaattatat ccgatatttt atttgtttta agtatatcta aactagtaga
     3601 aatttctatt ccaattaact tttttctaat atggatacta tcttatcttt tatttattat
     3661 agaagtgagt ataagcctta ctatagaaaa aggtgaagca acaattgaaa atatattcat
     3721 agttgcaata atgtatttta catactctca actatggctg tttgtagcta taaaaggaat
     3781 gatagagtat ctaaaagata taatttttaa aagagaggtt aaatggtata aaacagaaag
     3841 attttgattc atgtacagga ggaattaaca tgaaaaaatt tattatatca attatatctt
     3901 tagtattgtt tttttcaaat atatcactta tatacaaagt aaatgctgat gagactaaag
     3961 ttaaaaatta taagtttgaa agagatataa ctattgatgg tgttatagga agtaatagta
     4021 ctttttttga agtaaataaa aattgggata ttgaagaagt tttattgcat cttaatttta
     4081 gtaagagcca aatattaaat ggagatgttt caagtttaac tgtattgata aataatgtac
     4141 caataaaatc tattaaatta aatgctaaga caaattataa aaatacatta gaagtattag
     4201 ttccaaaaga ttatataatt caaggatata atgaaataaa aataaaaaca tacaaaacaa
     4261 tatctgataa aatttgccaa gatgattcca atactgggaa ctggatggtt attcacaaag
     4321 aatcatatat atcaattaga tataagcaga aaaaggtgga aaactcaata aatgaatatc
     4381 cgtatccata tgcagaaatt gaaaataatc ataaattaga tacaactata gtagtaccag
     4441 ataatatgac tagaggagag actacagcag tgtttaattt ggcatcagca tttggaaaaa
     4501 taacaaaaaa tgatgattta aagttagatg ttaaattata ttcagaaatg aaaaattggt
     4561 cagatgataa tattatatat attggtaaac ctgaaaatac agcagaagaa attttggata
     4621 ttttaagtat aaaggaacaa accctattat cttcaaactg tataatcaag caagtagatt
     4681 caccatacaa taagaataag aagatgatgg tggtaattgg tagtaatgaa gatgatttaa
     4741 taaaggcatc aaatttatta atagaaaata gactttctaa ccaagtttta tcatcatcag
     4801 tacttgtaaa caaagagact aatataaaaa ttaataggga acaaaaatta aatttaggtc
     4861 atttaacatt aaaagactta ggatattctg actttttgtt ggaaggagca tttaatcaac
     4921 aagctttgtt tgatgtcaaa ataccaacgg gtaaggtatt agatgatggt tcaaagatta
     4981 tcttaaattt aagatattct gacaatttag attttgaaaa atctttagtt acagtgtcta
     5041 taaatgatgt tattgtggga agtaaaaaat tggataggtc acattctaat aatgataagt
     5101 tagaattaaa aatacctaaa gatatagata ataaaaacta ttatcaagtt aaattgacat
     5161 ttaatttgag tataaaaaat tcgaattgtg ttactagaga gagtaataat ccttgggcat
     5221 atgtatcaaa taattcttat ttggcgttat ctacgaaaga aaatgaaacc ttatcttttg
     5281 aaaattatcc gtatccattt gttagagatg atgaatttaa tgatttaact gtgataatgc
     5341 cagattattc agggtcacaa gctatgacct ggatgtttag attaggtgtt actttaggtg
     5401 cgaatataaa ttcacataat ggaaatataa atgtaattag aggaaaagaa tttagtgaca
     5461 agtataagga tactaatatt gtagtttttg gagtgccaca taataattct gttataaaga
     5521 tgttaaataa taatttaaat ataaagttcg ataaaaatta ctccaatttt atatcaaatg
     5581 ataagataag ctttattgat gattatggaa aaaatatttc taccattcaa ttaataaaat
     5641 caccatataa taatcaaaaa aatatcatgg taattagttc aatgaatgaa aaaaacttat
     5701 atttaggaat ggattatttg ttgaataaaa gtaaagttaa tgatttaaaa ggagatacat
     5761 tgataattga tgaatacgga gaggtagaag atttagctta taatttaaaa tctaagaaag
     5821 aagtaaaaga ttcaagttgg aatatgagta taaataagac aactaaagta tttttaatga
     5881 tttcttttat tactataatt gtagtcatga tattatcaat gctatatata aaaaaatata
     5941 aaagaagata atatgataaa tttattattt taaaagaggt atataatttg aggaaatata
     6001 tgaataaatt taaattaaaa agaagtattt taatctcatt aataatctta atttttatat
     6061 ttatagttta ctttgtaagt attgagttaa aagataggag tctagtaaat aatcaatata
     6121 aaggtaagat taaatcggcc aatttatctg tagattatga aataaatcaa gttatgaagg
     6181 atatagataa attgggattg aatacagtaa atgtacctat aattataaat gtagagtcca
     6241 ttaattctga tattatgtca atagataata atagtaaaga aaaggcaatt aagttaataa
     6301 aaaaattaaa taaaaaaggt atttcaacaa tattagaggc atatccatgg attgaaaatg
     6361 gaaaattgta tgagactgac tggaacccta taaataaaaa aagatttttt tatacttggc
     6421 aaaatgccat attaaatgaa cttataattg atgtagcaaa tccactggat gtaaatgttt
     6481 taaatattgg ttctaatttt gtacatttgg aagaatatca acaaaattgg ggtgaaataa
     6541 tagattttgt acaatccaaa tttgacggtc ttgtaacgta cagaactaat tggtggtata
     6601 caaaaaaaga tgatttaagt agtaagttgt tttatgaaca aaagttgaac aataattttt
     6661 ttgataagtt agattttata tctatagcag catattttga gttaagtaat aaacctgtta
     6721 atacagtgga tgaattaatt tctgccttac actcatctac tgtaaacaat agaggacaaa
     6781 atataaaaca agaaatatat aacctttata aagctcatag aaaacctata ttttttggag
     6841 aattaggttt ttcaaataga gaaagtgcaa gttctcagcc gtggaatcat actccttcaa
     6901 aagctgtaaa tggcgaagaa caagcaaggt gttttgaggc atataagaaa gtttttgaaa
     6961 atgaagactg gataaatggt ttttctgtgt tttgtgtagg taaaatagat gacgaaaaaa
     7021 atttttaccc atcaaaagag agtataaaag ttattaagtc ttggtatgaa taattttgaa
     7081 agtgtagaat ttatatagat taaatagatg attaggagag atataaatga atgacatcaa
     7141 ggttatgact gtatttggaa ctagacctga agctatcaaa gtagcacctt taataaagga
     7201 acttgaaaaa agagagaata taaaaagtat tgtttgcgtt acagctcaac atagagaaat
     7261 gcttgaccaa gttatagaaa cctttaatat aaatgtagac tatgatttgg atataatgga
     7321 aaaaggacaa agcctgaatg atataacatg taagatttta aataaacttc cgttgatttt
     7381 aaacaaggaa aatccaaata taatattggt acatggagat acaaccacaa ctctagctac
     7441 aagtttgact gcattttata ataaaactct tgttggtcat atagaagctg ggcttagaac
     7501 ctatgataaa tattctcctt ttccagagga gttaaataga caacttacag gtattattgc
     7561 tgatatgcac tttgctccta ctaatttggc tagaaaaaac ttgatttctg aaggaaaacc
     7621 taataataat atatttgtaa ctggtaatac tgctattgat gctttaaaga tgacaataaa
     7681 agaaaactat aatcatccta taattgatga gattggtaac gataggatga ttttgttaac
     7741 ctctcacaga agagaaaatt tgggtaaacc tatgaaaaat atattcagag ctataaagcg
     7801 aatagtagat gactttgaag atgtacagat agtatatccc atacatttaa atcctaaaat
     7861 acgaactata gctgatgaga tatttggaaa atttcctgaa aaaattcata ttatagagcc
     7921 tttagatgta gctgattttc ataacttttt aaataaatct tatatgataa tgactgatag
     7981 tggaggaata caggaagaag ctccatcatt aggtaagcca gttcttgtgt tgcgtgataa
     8041 aacagaaaga acagaaggaa ttgaagcaaa gacacttaaa ttagtaggta ctaatgaaga
     8101 tagaatatat aattcagtga gcgatttatt aataaataaa gataactatg ttcagatgag
     8161 taaagcatct aatccatatg gagatggaaa tgcaagcaaa tatatagtgg atataataat
     8221 taaaaaattt aattgcaaat atttaaatta gataaggcct aaaacattaa ctaattgata
     8281 taagatttat gataatatta aggaatacaa tttttaaggg ggagtaatat ggctagaaaa
     8341 ttaataaaaa atttgggtaa aagtaaaagt gtaaagagag tgaaactttt atttaagaag
     8401 atttttatta ctgtattcat agtagcaagt atagttgcta tttttaatat aacgaaatat
     8461 tttgaggaat tatacaaagt aagggattta aaaagtacta aaattgaata ctatatggat
     8521 gtggctgatg aagcaggaga tggaaaggtc caattaagtt ggaaggcctt acttgctata
     8581 gacatggtaa tacatgatga agatttaagt aatataaaaa agaaagacac attggatata
     8641 ggggaaaagt ttatagtaga agataaaaat ggtaaaggcg aaaaggtgta taaggtaaaa
     8701 aagtttaata aggtattaag cgaattgaaa tttgactctt ctcaaaaaag tagagcaaga
     8761 aaatacatga aagatttaga atacacatac cttggaaata aacaattaga tagtagtgat
     8821 gaaaaaatta aatttataaa gaagttagaa gactcagcta taagagaata tattgattat
     8881 ggaatattgc cctctataac aattggacaa gctatattag aatctggttg gggaaattct
     8941 aaacttacaa aacagagtaa taatttattt ggtataaaag cagataaagc atggaaagga
     9001 aaaagtgtag aaatttcaac ttcagagcat tataatgaaa aaattgtagc tagttttagg
     9061 tcgtataatt cattacaaga ttctgtcaaa gatcacagtt tatttttaat taataataaa
     9121 agatatagaa aacatgggtt gtttgaggca aaagattata ttagtcaagc tcaagcatta
     9181 gaaaatgcag gatatagtac agcagaagat aaaaaaggaa atcgtatata tgcagagcta
     9241 ttaatagatg ttattaggag ttataatttg caattgatag ataataaagt tgagacaaaa
     9301 taattgttaa ctttatatga ggagataaca agataagatt tttttctaca aaaagaatag
     9361 aaaaattgta aaaaaaacta aaaaaatgta ttgacttgtt atttaaaagg tgatattatt
     9421 attactgtac tcgaaagaga acaaaa
//
LOCUS       DAKBNN010000148         9446 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000148 DAKBNN010000000
VERSION     DAKBNN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.149"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(228..389)
                     /locus_tag="OUB02_002869"
     CDS             complement(228..389)
                     /locus_tag="OUB02_002869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886608.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            complement(468..968)
                     /gene="tcdE"
                     /locus_tag="OUB02_002870"
     CDS             complement(468..968)
                     /gene="tcdE"
                     /locus_tag="OUB02_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HCT8886609.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            complement(1090..8190)
                     /gene="tcdB"
                     /locus_tag="OUB02_002871"
                     /gene_synonym="toxB"
     CDS             complement(1090..8190)
                     /gene="tcdB"
                     /locus_tag="OUB02_002871"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HCT8886610.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            complement(8508..9062)
                     /gene="tcdR"
                     /locus_tag="OUB02_002872"
     CDS             complement(8508..9062)
                     /gene="tcdR"
                     /locus_tag="OUB02_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HCT8886611.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
BASE COUNT         3205 a         1541 c          905 g         3795 t
ORIGIN      
        1 taatttgtaa acatatctta tattttttta atgtttttat ttctttatat atttttccta
       61 taacttttaa actatcaatt aaatcattta ttaaggtttg atacttattt tctatatcat
      121 tttgtccttc atcacgtatt tttgtgtttt tgaataattt ccatcacaat ttacacatat
      181 ttctatgttt aactattata gtttgtgata tctgtaacta atgtagctta atagatttta
      241 ccaactgacc atgcaccatc tttgtgaacc aatgtttgat atactacctt gtcatctata
      301 tagtaatgaa cacttgcttc gaaattatat gtatttaata cttgtgtgtc tcttggcatc
      361 tgctccctcg ttttcattat tagtttcatg aattatgata actttcagct attcttattt
      421 ggataacact ttttatttgt taatatcttt attatatcta ccattactta cttttcatcc
      481 ttagcattca tttcatctgt cattgcatct agtaaaattg ctattttttc ctttaatctc
      541 ttaggtactg gtaatccaca taagcacata ttttttaata tacttacact ttcatataaa
      601 ataaataaaa tagagaaaaa ttcagatatt ccaagatgtt ttagtcttaa aaaattgata
      661 caatcttgtg gtaacataaa taaaaagtta aactttgtaa gaatgtcaac tactgataaa
      721 aaaaatatac atgctatcat tgctactttt ctgattcctc catctattcc aaaactagaa
      781 ttaaatttac gactttttat tgcacttaaa catcctaata atgtatcaaa tgatatagtt
      841 aaaattacta actttataaa tatatgctct gataaaaaag atattgtcat attcataacg
      901 cctcctaggt ttatataaaa aaatattttg ttaccattag aaatataaaa aggtgaacta
      961 ctgtgcattc atcatagtcc acctttttat tggtaaatct tcatattaga tagaacactt
     1021 tatgtagtgt atcattttgt ttatttttat atatttaagt ataagaggat atatttaaca
     1081 tatttttatc tattcactaa tcactaattg agctgtatca ggatcaaaat aatactcctc
     1141 accatcaata ataactgaac cagttgctgc aatatattca tctgtaaaat aatatctctt
     1201 ttcatctaaa tctaaccaac cagtatagtt tattgattct ccctcaaaat tctcatccaa
     1261 agtattttga tgtgcaaagt atttaaatcc atctggtgta ttaaatactc caatctgcat
     1321 gacaccatct tcaccaaaat agaacatctt atcttctata tttatataac caaattgcat
     1381 ttcaccattc tcattaaagt aataattatt attttcaaat gatataagac ccgttctcat
     1441 tatgcccttc tcatcaaaat aatattttat atcatcaatt aaattaatac ctttgcatgc
     1501 ttttttagtt tctggattga aataatattt atcactttca ttttccatat catatatcca
     1561 tccagtctca attgtatatg tttctccaaa ataatataca tcttccccaa ctctaactaa
     1621 accactatat tcaactgctt gtccgtaaat attatcattt acagtattag caggtgcaaa
     1681 atatttatat ccatctgaag tatcaaatac accaatttga actataccat tatcatctat
     1741 atagaaataa ttgtcatcta tgttttgtac tccagattct ataattccag agtcagagaa
     1801 gtagaagact ttatcattta tagtgacaaa tccaacttgc ataattccat catcattaaa
     1861 ataatattga ccatcattta ttaatgacaa acctatatat gcttctgctg tatcttcatc
     1921 aaaataatac tttgaaccat cctctaaatc tttccatcca actacagctg taaatgaatc
     1981 atcaaaatag taaattttat tattgaaatt taatatacca gaatatgaga tttcttcacc
     2041 ttcttcattt cctaaatctt cattatgatg agcaaaatat ttaaatccat cttctgtatt
     2101 aaatactcct atttgcattt ctccgttttc agcaaagtag aaatgcttgc catctatttc
     2161 agtgtaacct actttcataa caccagaatc atcaaaatag tgtttattat catttatact
     2221 aacaaatcct ttttgcataa ctccatcaga attgaaatag tatttataat cacctatttg
     2281 atttagacct ttaaaagctt tacctgtttc tgggctaaaa tagtgcattt caccatctaa
     2341 ttctttccat tctacagctc ctctataatt atcatcaaaa taataaatat tttcgtcaat
     2401 aattaatttt ccagtaaaat caattgcttc tccttctagg ttttcatcaa gtgtattagc
     2461 tggggcaaaa tatttaaatc catcttctgt actaaataca cctgtttgta acactccact
     2521 ttggttgaaa taataatttt tgtcatcaat tattgtctct ccaattgaag cagctccacc
     2581 attaattgga ttaaagtagt atttatcatc gcctacagtc acaaatccag ttatcaaatt
     2641 atttactggt ggtttaaaat aatataatga atcattaata tatattaatc cagataccat
     2701 cattccaaag ttattaatat aaaatttctc attatataaa gaaactagac ccaaatcata
     2761 gccaatcaat ccatcctcat aatatgaagg tgtaaatgat aagattattt cacttacagg
     2821 tacatcttgt ttatcactaa agttaaaaga tagcttattt gccaaagtct tatctttaaa
     2881 aacattaacg aatcttattt taacttgtga caccttattt tcttcactag ttgacataag
     2941 aataaaatca ttaccatcat tactccatac atatcgtata gatagatcat tgatattaac
     3001 atttattttt tcatttatat aatttgcatc taatacaata acttctggat aagtattatt
     3061 tgtttcatat acaggcgtta tatttatttc atctgtataa atatttggtg aaattacaac
     3121 tttattaaca caactgtcta ttccataaag atacttttga gagaaattga taacagttga
     3181 agatatatct ccagaatcat ctaaatcata atttggttcc actatcatat tttgtctatt
     3241 tcctacatat aaagtataat tagtttctag tgtattaaac ttaatgaaat atggttgtat
     3301 attatcattt tcatcacaaa taaactcaaa ttggccaata gaagtagtac cacttattat
     3361 aaaattagca tctaatataa acttaatatt agattgtaag aaattaacga aaatactttt
     3421 tatattcata ctttctaaaa agctcattaa agaatctgaa gtatttgtat taccttttct
     3481 attcatgaac ttcaaaatct cagctactcc actttcatct aaatgcacac tatttaactt
     3541 tatagttttt tcatcttgta gagtcaaaga aagagagatt tttatatcat ccttaagata
     3601 ataacctgtt aatatattaa cattatcttt agttataact ttgacatctt tcaaattatt
     3661 actataatat ccaaatgaag gcttactatc atccatataa accttactta taagaactac
     3721 atcaggtaat tcagatacaa ataaaccttc ttttgttgaa ccattaataa aaccattctc
     3781 ttttccttca ctatctacaa agctatatgg tatatttttc tgtaattcgc tattgaatcc
     3841 tatataatct attttctgtt gaatatgatt tgaatttaac atcaatattt ttaattcgcc
     3901 agaaataagt aatttatatg atttagataa taaatcaact tctataattg catttattcc
     3961 ttctaaaatt gaaaatgtta aagaaacaaa tccattactt ccatttacct caccagaaaa
     4021 attaatctca tggctattta agataatttt attctcttca atacttagtg tagataaaat
     4081 accttctatt aaatcacctt ttttaatttt atcagattct atagttacat ctctcacaac
     4141 attatcaaca tctataatcc aaacatcact ttcacttaat tctatattta tacccatatt
     4201 atattgagaa agagacaatg cataagttcc tcctgaacca tagaaagaat atgataattt
     4261 ttctcttata tattctgtag ttattattgg aactataaaa cttctagtat tactatctaa
     4321 atttattctt atattagtat cttcatatct tggttttaat gttgttatta aagcatcagc
     4381 tataaaagca aaatatctcc aataaaactc accttcatag ttatctctta tacggtctaa
     4441 cagttttgtg ccatcatttt ctaagcttct taaacctggt gtccatcctg tttcccaagc
     4501 aaatactcta tttggagcat taggtaatac cattaaatct tttgacaaat caagttcttc
     4561 tttttgtact tccaatacgt catatataga taagtgtggc tctctatatg ttattgatgg
     4621 tgctgaaaag aagtgatcta tatcatcagt tacagtatga cctgaaccac cttccattct
     4681 ccagatttca catttaccta aaactattga attattatta aaatctattt ctgatatcac
     4741 taaatcatct tgtggcatca ttattttatc atctaataaa gtaaatactc cttcagtttc
     4801 aactaatgaa acatgtttaa aataatctac aacctttgtt gccttatctc gaagtacaag
     4861 ttcattgttt actaagcttg gtatacctgc tgaaattcct gctaaaggaa ctaaaagtat
     4921 actaaatcca ctagctaccc ccaaagatga agtaatgatt gcagttgtag ctgttgttaa
     4981 atttactgcc attataccta tcttagcttc tatttcttgt cttaataatg ggtcactcgt
     5041 ttcacttagc tctttgattg ctgcacctaa acttacacca tctataatag ttgcaattat
     5101 aggtaatcct tcagataatg taggaagtaa gtctatagtt tcatctaatg cagttgatac
     5161 taattcaaca actttggctg catctgtaat agtatttaaa ccagtactaa ataattgagc
     5221 gtaaacttgg actttcattg ctacacttaa attactaaga gattctttag aactattata
     5281 ttctattaat gattgtataa aaaatgcagc atttaaagta tttacttcgt gtgtagtatc
     5341 taaatttact ttttttacta acttaccatt tacagtatca aatatagtac cttttatctt
     5401 agaaatctct tcagttatat gattagcata ttcagagaat attgtttttt cagtttctac
     5461 aaatatagat tctccagttt ctttattaat aaatcttata ctaaatccct catcagtctc
     5521 tgatatgtcc tcaaaagata taaaatgaga atcttctaat tcattctgtt gttttaagtc
     5581 acatagtgca tcagaaatag attctattag tttaaattca tcttttatat aattaataga
     5641 gtcagaagtt aaattcttag cttcttcaat cctttcctca acaatttgcg tatctatatt
     5701 tgaaataaca tttatctcac attctgttaa cattactttt tcttctagtt caatatcact
     5761 tgaattagaa ttatttctaa tttcttgtaa taatgtagat agctcaggta aatttttaga
     5821 ttttactgta attttatttt ctttaggatt aaatgatata tattcttttg atgaaatatc
     5881 ctttataata ctttcttctt tatttatcca ttcaccagaa tgatccaata attctcttct
     5941 tccttcacta tttattctaa cttcatattg atttgcactt actataatag agtcttgact
     6001 tatagatggc attaattctg atattttatc tttaacttta agtaataatt ttccaggata
     6061 agtctcctct acgttgatag agtagctaaa catattacat cctaataaat ttatttctat
     6121 tgacttagga gaaatatcct ctttagctaa atctattgct gcttctattt ctgtggataa
     6181 tgaatctaca tcaaaacctg caaatatatc agtattaaat tcatctttac catgaccaat
     6241 aaatgttaat ttaatcttag gtctatcaga aattatactt ggaattttat acttgtctat
     6301 ttcttgtatt tcaccatctc caggattata ataatatgca atttctgaat cttctatatt
     6361 tttctgaaat agtacactat cataaggagt ctttgcaaat aagttacatg ctgcttcata
     6421 actaatttta tctccttgta actgaacaat atagtgtata tatcctctct ctgaacttct
     6481 tgctaatgaa gatatttttt ctaaaagata ctccttgtca actactatat tttgagaaaa
     6541 atcaagatta tcatcttcac caagagaacc ttcaaaataa ttccttttat attcttcaaa
     6601 ttgagcttta gctcttgcat cgtcaaatga ccataagcta gccatttctt gttcagttga
     6661 ttgagaaata ttagttttag agatttcaaa gtttcttaaa tcagcttcta tcaaatggat
     6721 attcatactg ccttctttaa acattaataa atcttgataa gctgccgcat atgcttcagg
     6781 gccacttaag ttaatagtag ttttaacatc tgggaagaaa ccaactctta aatactttcc
     6841 tagttccatc ataaatctac cattatctgc attagcttca gccattatac tatcaataaa
     6901 ggtattcgtt gtagtattaa aatcattatc ctcgctaata gctggattta aactattatt
     6961 caatatttta tatctattct cgatttgttt tactattaaa ttgctacaat atgagtcttt
     7021 cacagaaatt agcccttgat ttataatacc cttactatta aatgcaattt taacttctag
     7081 tggtgatgcc tccatatcac caagtgatga gaatatttct gatttatctg acttagaagc
     7141 tagaacagat tcaaaactac tttgaacttc ttcgtctaac atgtcaaaat gttctgaggt
     7201 atattctggt atatattctt tgtatttcat tatagcttct aactttgtca tttcccaaaa
     7261 atccactgtt actgaactag gtttctctat agactcaaat aagtctggtt gtattcctgg
     7321 taacatatca acatctaaat acataccacc aatttctttt aatgcagata ttcttaatat
     7381 gtcagaagca gcagctaaat tccacctttc taccaactct tgttcatata agttgaatga
     7441 ctctccattt ttaaattctt caaagtttct aacatcattt ccactattct gtgtaatttt
     7501 atttaaggat tcttcaatat aggtattaag ttcatctatc tcctttgaat actcatttga
     7561 aagatatgtc tttacaatat catcaattat aagttcagga ttttcttctc tttgagcttt
     7621 atagtagttt atgaaatttt tctgtttatc ataaattatt tccatacgtt ttctgaagaa
     7681 tttattatag tcaaatctag ggtcatttaa gttttctcta aatgattcaa gtgtatcatt
     7741 tattgctgat tctactacag tttttttcaa tgtgtttatc aaaaatgcat tactatcata
     7801 aaaaacatta acattataat cactatttac atctttccat tgatttatat aattaatagc
     7861 agtgtcattt atttgacctc caatccaaac aaaatgtaaa tttttctcaa ctggagttaa
     7921 attattattc tttagctcta atacttctgt aactagatat tccttaaatt tttttaaggc
     7981 tttatttcta ccagattttt tatatgtatc tatataaata tctgttaaac tatttatatc
     8041 ttttaatttt aaatattttt cgactacagt attctctgac atattatgat attcttctaa
     8101 agcatccaat attgcaacat attcatcttc ttgagtacga aatcttacat ttgccatttt
     8161 ttctaactgt tttctattaa ctaaactcat aaaattttct cctttactat aatattttta
     8221 catctaaatg ctaaaactct tttatatatc ctcctttcta tttataaaat atactaatat
     8281 ctacttaggt tttcatatac attcacttcc taacatttta aatttaagac actcagttga
     8341 ttaatttgct cttcgattga ataaagacgt tgtctgaaat aaatatgtaa actttgttct
     8401 atataaaata taaaaattaa gatattctta gattaagtta ttgattttac aataaagtta
     8461 attctaaaat ttgatttcta ttgtaaatat ttctttaagc tcattaatta caagttaaaa
     8521 taattttcat agtctttttt tattttttta aatgctctat ttttagcctt attaacagct
     8581 tgtctagata tttttaattt cactgatatt tctctatcag aatatccttt taaatattta
     8641 taaaaaataa cttttctttg aataccagat aattctttca aaatatattc gaaaaaacaa
     8701 atatcttcct caaaaacaga cttactttga taattatttt tcatatttaa gatattttca
     8761 tcaaattcta caaaattaaa cttcatttta gaatatcttc tagacaactc aaaagtctta
     8821 ttcagtaatg atttatgaat atatttgact ataatagcat cgcttttttc tgaaaagcta
     8881 cttaatttaa tattttttat taattcaata aaaaatatta ttaaatctgt ttctccctct
     8941 tcataatgta aaactctact aagttttttt actaatggct taaacataaa taaaatttct
     9001 tgcaaatcat ctactgagtt atttcttgct aaaacaatta attcataaaa agacttttgc
     9061 ataaaatcat cctctcttat atttataatg atgatttatt tgaaaatttt gacaacatcg
     9121 gaattaaatc agtcattata attatataat cggcaaataa attaaatttt atatgaaaat
     9181 atgcattatg aatacattaa ttttaataat taattagtaa tctttttcta ataaataaag
     9241 tattttagca ataaacttta tattaataaa gtattgtaaa aacaaatcat tttattaaag
     9301 taaaataaaa aaaatctata tgacatgttt ttatatttat atttcttttt tcttaatttt
     9361 cattcttttt tgaagttata actagatttt aaaatagatc aattttcaag taacaattac
     9421 ataattatta aataattatg taaact
//
LOCUS       DAKBNN010000149         9430 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000149 DAKBNN010000000
VERSION     DAKBNN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.150"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            217..8349
                     /gene="tcdA"
                     /locus_tag="OUB02_002873"
                     /gene_synonym="toxA"
     CDS             217..8349
                     /gene="tcdA"
                     /locus_tag="OUB02_002873"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HCT8886612.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(8684..9382)
                     /gene="tcdC"
                     /locus_tag="OUB02_002874"
     CDS             complement(8684..9382)
                     /gene="tcdC"
                     /locus_tag="OUB02_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HCT8886613.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPSCSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         3673 a         1162 c         1373 g         3222 t
ORIGIN      
        1 ttgagtatct ttaaattgaa atgttaggaa gtgatttaaa tatgaaaact taaattataa
       61 aaaatcaata ttaatttatt tttaaaaaat agaaaggagt gtataagatt tattttcaaa
      121 gtttaaaaac aagaaaatca atttaaattt cagaaggaat aaatgtggtt atagaagtgg
      181 atttattatc aaaaataata atactaggag gtttttatgt ctttaatatc taaagaagag
      241 ttaataaaac tcgcatatag cattagacca agagaaaatg agtataaaac tatactaact
      301 aatttagacg aatataataa gttaactaca aacaataatg aaaataaata tttacaatta
      361 aaaaaactaa atgaatcaat tgatgttttt atgaataaat ataaaacttc aagcagaaat
      421 agagcactct ctaatctaaa aaaagatata ttaaaagaag taattcttat taaaaattcc
      481 aatacaagcc ctgtagaaaa aaatttacat tttgtatgga taggtggaga agtcagtgat
      541 attgctcttg aatacataaa acaatgggct gatattaatg cagaatataa tattaaactg
      601 tggtatgata gtgaagcatt cttagtaaat acactaaaaa aggctatagt tgaatcttct
      661 accactgaag cattacagct actagaggaa gagattcaaa atcctcaatt tgataatatg
      721 aaattttaca aaaaaaggat ggaatttata tatgatagac aaaaaaggtt tataaattat
      781 tataaatctc aaatcaataa acctacagta cctacaatag atgatattat aaagtctcat
      841 ctagtatctg aatataatag agatgaaact gtattagaat catatagaac aaattctttg
      901 agaaaaataa atagtaatca tgggatagat atcagggcta atagtttgtt tacagaacaa
      961 gagttattaa atatttatag tcaggagttg ttaaatcgtg gaaatttagc tgcagcatct
     1021 gacatagtaa gattattagc cctaaaaaat tttggcggag tatatttaga tgttgatatg
     1081 cttccaggta ttcactctga tttatttaaa acaatatcta gacctagctc tattggacta
     1141 gaccgttggg aaatgataaa attagaggct attatgaagt ataaaaaata tataaataat
     1201 tatacatcag aaaactttga taaacttgat caacaattaa aagataattt taaactcatt
     1261 atagaaagta aaagtgaaaa atctgagata ttttctaaat tagaaaattt aaatgtatct
     1321 gatcttgaaa ttaaaatagc tttcgcttta ggcagtgtta taaatcaagc cttgatatca
     1381 aaacaaggtt catatcttac taacctagta atagaacaag taaaaaatag atatcaattt
     1441 ttaaaccaac accttaaccc agccatagag tctgataata acttcacaga tactactaaa
     1501 atttttcatg attcattatt taattcagct accgcagaaa actctatgtt tttaacaaaa
     1561 atagcaccat acttacaagt aggttttatg ccagaagctc gctccacaat aagtttaagt
     1621 ggtccaggag cttatgcgtc agcttactat gatttcataa atttacaaga aaatactata
     1681 gaaaaaactt taaaagcatc agatttaata gaatttaaat tcccagaaaa taatctatct
     1741 caattgacag aacaagaaat aaatagtcta tggagctttg atcaagcaag tgcaaaatat
     1801 caatttgaga aatatgtaag agattatact ggtggatctc tttctgaaga caatggggta
     1861 gactttaata aaaatactgc cctcgacaaa aactatttat taaataataa aattccatca
     1921 aacaatgtag aagaagctgg aagtaaaaat tatgttcatt atatcataca gttacaagga
     1981 gatgatataa gttatgaagc aacatgcaat ttattttcta aaaatcctaa aaatagtatt
     2041 attatacaac gaaatatgaa tgaaagtgca aaaagctact ttttaagtga tgatggagaa
     2101 tctattttag aattaaataa atataggata cctgaaagat taaaaaataa ggaaaaagta
     2161 aaagtaacct ttattggaca tggtaaagat gaattcaaca caagcgaatt tgctagatta
     2221 agtgtagatt cactttccaa tgagataagt tcatttttag ataccataaa attagatata
     2281 tcacctaaaa atgtagaagt aaacttactt ggatgtaata tgtttagtta tgattttaat
     2341 gttgaagaaa cttatcctgg gaagttgcta ttaagtatta tggacaaaat tacttccact
     2401 ttacctgatg taaataaaaa ttctattact ataggagcaa atcaatatga agtaagaatt
     2461 aatagtgagg gaagaaaaga acttctggct cactcaggta aatggataaa taaagaagaa
     2521 gctattatga gcgatttatc tagtaaagaa tacatttttt ttgattctat agataataag
     2581 ctaaaagcaa agtccaagaa tattccagga ttagcatcaa tatcagaaga tataaaaaca
     2641 ttattacttg atgcaagtgt tagtcctgat acaaaattta ttttaaataa tcttaagctt
     2701 aatattgaat cttctattgg tgattacatt tattatgaaa aattagagcc tgttaaaaat
     2761 ataattcaca attctataga tgatttaata gatgagttca atctacttga aaatgtatct
     2821 gatgaattat atgaattaaa aaaattaaat aatctagatg agaagtattt aatatctttt
     2881 gaagatatct caaaaaataa ttcaacttac tctgtaagat ttattaacaa aagtaatggt
     2941 gagtcagttt atgtagaaac agaaaaagaa attttttcaa aatatagcga acatattaca
     3001 aaagaaataa gtactataaa gaatagtata attacagatg ttaatggtaa tttattggat
     3061 aatatacagt tagatcatac ttctcaagtt aatacattaa acgcagcatt ctttattcaa
     3121 tcattaatag attatagtag caataaagat gtactgaatg atttaagtac ctcagttaag
     3181 gttcaacttt atgctcaact atttagtaca ggtttaaata ctatatatga ctctatccaa
     3241 ttagtaaatt taatatcaaa tgcagtaaat gatactataa atgtactacc tacaataaca
     3301 gaggggatac ctattgtatc tactatatta gacggaataa acttaggtgc agcaattaag
     3361 gaattactag acgaacatga cccattacta aaaaaagaat tagaagctaa ggtgggtgtt
     3421 ttagcaataa atatgtcatt atctatagct gcaactgtag cttcaattgt tggaataggt
     3481 gctgaagtta ctattttctt attacctata gctggtatat ctgcaggaat accttcatta
     3541 gttaataatg aattaatatt gcatgataag gcaacttcag tggtaaacta ttttaatcat
     3601 ttgtctgaat ctaaaaaata tggccctctt aagacagaag atgataaaat tttagttcct
     3661 attgatgatt tagtaatatc agaaatagat tttaataata attcgataaa actaggaaca
     3721 tgtaatatat tagcaatgga ggggggatca ggacacacag tgactggtaa tatagatcac
     3781 tttttctcat ctccatctat aagttctcat attccttcat tatcaattta ttctgcaata
     3841 ggtatagaaa cagaaaatct agatttttca aaaaaaataa tgatgttacc taatgctcct
     3901 tcaagagtgt tttggtggga aactggagca gttccaggtt taagatcatt ggaaaatgac
     3961 ggaactagat tacttgattc aataagagat ttatacccag gtaaatttta ctggagattc
     4021 tatgcttttt tcgattatgc aataactaca ttaaaaccag tttatgaaga cactaatatt
     4081 aaaattaaac tagataaaga tactagaaac ttcataatgc caactataac tactaacgaa
     4141 attagaaaca aattatctta ttcatttgat ggagcaggag gaacttactc tttattatta
     4201 tcttcatatc caatatcaac gaatataaat ttatctaaag atgatttatg gatatttaat
     4261 attgataatg aagtaagaga aatatctata gaaaatggta ctattaaaaa aggaaagtta
     4321 ataaaagatg ttttaagtaa aattgatata aataaaaata aacttattat aggcaatcaa
     4381 acaatagatt tttcaggcga tatagataat aaagatagat atatattctt gacttgtgag
     4441 ttagatgata aaattagttt aataatagaa ataaatcttg ttgcaaaatc ttatagtttg
     4501 ttattgtctg gggataaaaa ttatttgata tccaatttat ctaatactat tgagaaaatc
     4561 aatactttag gcctagatag taaaaatata gcgtacaatt acactgatga atctaataat
     4621 aaatattttg gagctatatc taaaacaagt caaaaaagca taatacatta taaaaaagac
     4681 agtaaaaata tattagaatt ttataatgac agtacattag aatttaacag taaagatttt
     4741 attgctgaag atataaatgt atttatgaaa gatgatatta atactataac aggaaaatac
     4801 tatgttgata ataatactga taaaagtata gatttctcta tttctttagt tagtaaaaat
     4861 caagtaaaag taaatggatt atatttaaat gaatccgtat actcatctta ccttgatttt
     4921 gtgaaaaatt cagatggaca ccataatact tctaatttta tgaatttatt tttggacaat
     4981 ataagtttct ggaaattgtt tgggtttgaa aatataaatt ttgtaatcga taaatacttt
     5041 acccttgttg gtaaaactaa tcttggatat gtagaattta tttgtgacaa taataaaaat
     5101 atagatatat attttggtga atggaaaaca tcgtcatcta aaagcactat atttagcgga
     5161 aatggtagaa atgttgtagt agagcctata tataatcctg atacgggtga agatatatct
     5221 acttcactag atttttccta tgaacctctc tatggaatag atagatatat caataaagta
     5281 ttgatagcac ctgatttata tacaagttta ataaatatta ataccaatta ttattcaaat
     5341 gagtactacc ctgagattat agttcttaac ccaaatacat tccacaaaaa agtaaatata
     5401 aatttagata gttcttcttt tgagtataaa tggtctacag aaggaagtga ctttatttta
     5461 gttagatact tagaagaaag taataaaaaa atattacaaa aaataagaat caaaggtatc
     5521 ttatctaata ctcaatcatt taataaaatg agtatagatt ttaaagatat taaaaaacta
     5581 tcattaggat atataatgag taattttaaa tcatttaatt ctgaaaatga attagataga
     5641 gatcatttag gatttaaaat aatagataat aaaacttatt actatgatga agatagtaaa
     5701 ttagttaaag gattaatcaa tataaataat tcattattct attttgatcc tatagaattt
     5761 aacttagtaa ctggatggca aactatcaat ggtaaaaaat attattttga tataaatact
     5821 ggagcagctt taactagtta taaaattatt aatggtaaac acttttattt taataatgat
     5881 ggtgtgatgc agttgggagt atttaaagga cctgatggat ttgaatattt tgcacctgcc
     5941 aatactcaaa ataataacat agaaggtcag gctatagttt atcaaagtaa attcttaact
     6001 ttgaatggca aaaaatatta ttttgataat gactcaaaag cagtcactgg atggagaatt
     6061 attaacaatg agaaatatta ctttaatcct aataatgcta ttgctgcagt cggattgcaa
     6121 gtaattgaca ataataagta ttatttcaat cctgacactg ctatcatctc aaaaggttgg
     6181 cagactgtta atggtagtag atactacttt gatactgata ccgctattgc ctttaatggt
     6241 tataaaacta ttgatggtaa acacttttat tttgatagtg attgtgtagt gaaaataggt
     6301 gtgtttagta cctctaatgg atttgaatat tttgcacctg ctaatactta taataataac
     6361 atagaaggtc aggctatagt ttatcaaagt aaattcttaa ctttgaatgg taaaaaatat
     6421 tactttgata ataactcaaa agcagttacc ggatggcaaa ctattgatag taaaaaatat
     6481 tactttaata ctaacactgc tgaagcagct actggatggc aaactattga tggtaaaaaa
     6541 tattacttta atactaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa
     6601 aaatattact ttaatactaa cactgctata gcttcaactg gttatacaat tattaatggt
     6661 aaacattttt attttaatac tgatggtatt atgcagatag gagtgtttaa aggacctaat
     6721 ggatttgaat attttgcacc tgctaatacg gatgctaaca acatagaagg tcaagctata
     6781 ctttaccaaa atgaattctt aactttgaat ggtaaaaaat attactttgg tagtgactca
     6841 aaagcagtta ctggatggag aattattaac aataagaaat attactttaa tcctaataat
     6901 gctattgctg caattcatct atgcactata aataatgaca agtattactt tagttatgat
     6961 ggaattcttc aaaatggata tattactatt gaaagaaata atttctattt tgatgctaat
     7021 aatgaatcta aaatggtaac aggagtattt aaaggaccta atggatttga gtattttgca
     7081 cctgctaata ctcacaataa taacatagaa ggtcaggcta tagtttacca gaacaaattc
     7141 ttaactttga atggcaaaaa atattatttt gataatgact caaaagcagt tactggatgg
     7201 caaaccattg atggtaaaaa atattacttt aatcttaaca ctgctgaagc agctactgga
     7261 tggcaaacta ttgatggtaa aaaatattac tttaatctta acactgctga agcagctact
     7321 ggatggcaaa ctattgatgg taaaaaatat tactttaata ctaacacttt catagcctca
     7381 actggttata caagtattaa tggtaaacat ttttatttta atactgatgg tattatgcag
     7441 ataggagtgt ttaaaggacc taatggattt gaatactttg cacctgctaa tactcataat
     7501 aataacatag aaggtcaagc tatactttac caaaataaat tcttaacttt gaatggtaaa
     7561 aaatattact ttggtagtga ctcaaaagca gttaccggat tgcgaactat tgatggtaaa
     7621 aaatattact ttaatactaa cactgctgtt gcagttactg gatggcaaac tattaatggt
     7681 aaaaaatact actttaatac taacacttct atagcttcaa ctggttatac aattattagt
     7741 ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct
     7801 gatggatttg aatactttgc acctgctaat acagatgcta acaatataga aggtcaagct
     7861 atacgttatc aaaatagatt cctatattta catgacaata tatattattt tggtaataat
     7921 tcaaaagcgg ctactggttg ggtaactatt gatggtaata gatattactt cgagcctaat
     7981 acagctatgg gtgcgaatgg ttataaaact attgataata aaaattttta ctttagaaat
     8041 ggtttacctc agataggagt gtttaaaggg tctaatggat ttgaatactt tgcacctgct
     8101 aatacggatg ctaacaatat agaaggtcaa gctatacgtt atcaaaatag attcctacat
     8161 ttacttggaa aaatatatta ctttggtaat aattcaaaag cagttactgg atggcaaact
     8221 attaatggta aagtatatta ctttatgcct gatactgcta tggctgcagc tggtggactt
     8281 ttcgagattg atggtgttat atatttcttt ggtgttgatg gagtaaaagc ccctgggata
     8341 tatggctaaa atatatgttt gataaaaaat tattcctgtg ctactaagaa attattttta
     8401 tataataaat attgagattt aattaaaagt catgtgttat tgtaatacat gacttttagt
     8461 taaaattttt ctatcattta ataatctatt attcttgact attttataat aaaattcata
     8521 tatggaaata ttaatactaa ataattaata gttgataaaa aatagataat atgctaaaag
     8581 caaaaactaa tttagagcct tgtaactgtt tatttgcaat tataaaaaca tctttaaaca
     8641 tattgactat aatataaaat attaactata atacaaaaca atattaatta attttctcta
     8701 cagctatccc tggtatagtt atatttccac ccatagttga ttcataagtt atagttccag
     8761 cacttatacc tcttatagtt atgtaatcat cctctaatac tcttgaagga gttattgatt
     8821 ttttataact acatagtaca accttatcat aatttccaga cacagctaat cttatttgca
     8881 cctcatcacc atcttcaata acttgaataa ccttaccttc aaattttacc tttttgtact
     8941 tataatcatc aggtgttcta gctaattggt cataagtaat accagtatca tatcctttct
     9001 tctcctcttc ttcttttttg cgttgttctt cagccttttt agcttcttca gcttttttag
     9061 cttcttcagc tttacgttga ttttcagctt caatagcttt cttttcgtcg tctttcattt
     9121 tgaaccatgg ttcagcatca gacaatttgc tatttaaagt ttctatttct gttttttgag
     9181 aatctataac tgatgtttgg ttagaaatga cctcctcatg gtcttcagaa caagatggtg
     9241 aggatatatt gccaaaaaac acgcctaaaa taaatgccag tagagcaata ccctttgtgc
     9301 tcttaaagaa tttaattatt ttcttagagc ttccttttcc ttcattacta aattcgttac
     9361 cctcattttt tttagaaaac ataatacaat ctccctttta ttagatatat tttattttaa
     9421 aagttaaaaa
//
LOCUS       DAKBNN010000150         9405 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000150 DAKBNN010000000
VERSION     DAKBNN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.151"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..169
                     /locus_tag="OUB02_002875"
     CDS             <1..169
                     /locus_tag="OUB02_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886614.1"
                     /translation="LKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLK
                     KYIDDMVEKLK"
     gene            383..1222
                     /locus_tag="OUB02_002876"
     CDS             383..1222
                     /locus_tag="OUB02_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCT8886615.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            1739..3595
                     /locus_tag="OUB02_002877"
     CDS             1739..3595
                     /locus_tag="OUB02_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCT8886616.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    3678..3918
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    3984..4219
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4304..5521
                     /locus_tag="OUB02_002878"
     CDS             4304..5521
                     /locus_tag="OUB02_002878"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCT8886617.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            5638..6120
                     /gene="cdeM"
                     /locus_tag="OUB02_002879"
     CDS             5638..6120
                     /gene="cdeM"
                     /locus_tag="OUB02_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HCT8886618.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            6385..7650
                     /gene="hisD"
                     /locus_tag="OUB02_002880"
     CDS             6385..7650
                     /gene="hisD"
                     /locus_tag="OUB02_002880"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCT8886619.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(7853..8605)
                     /locus_tag="OUB02_002881"
     CDS             complement(7853..8605)
                     /locus_tag="OUB02_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8886620.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(8723..8992)
                     /locus_tag="OUB02_002882"
     CDS             complement(8723..8992)
                     /locus_tag="OUB02_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886621.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
BASE COUNT         3745 a          943 c         1617 g         3100 t
ORIGIN      
        1 tttaaaatac tttttggaag atgaatcttg ggtagctata agaccatctg gaacagaacc
       61 aaaacttaaa ttttacatag ctgtaaaagg agctagtgat attgaagcag ataaaaagat
      121 tcaaggtttg aaaaagtata tagatgacat ggtcgaaaaa ttaaaataaa atattattat
      181 tttataagat aattttgtaa taatatttat ttagctgaaa attataattt actttcattg
      241 agtagaatat ggaaatgtta gtagaattgt tattttcagc ttttatttgt gttattttta
      301 cttaaataat tatattatag aatgaatctg agtataatta atattgtata atgaaattaa
      361 tttgatagaa aaggggatat ttatgtttgg tatagataag aaattgattg aatatataat
      421 tgcaaaatcc aattacagtg aattaagttc aaagtcagca gaagtatctt tcttcttaat
      481 gttgtccata tttccttttt taatatttac aataagttct atagcttata taccgattct
      541 tcatttaaat aagtatatag ctttgtttag aaatatgatg ccagaaggtg cttttgctgt
      601 gttatcttca atcatagttt ctgcaataga caatagaaac ctgaaatttt tagcagtcag
      661 ttttgtactt actatgtgga cattttcaag agcagtaaaa gctcttatta aaggaatgaa
      721 tagagcctat aaagttaaag aaactaggtc tttttttaaa attttatcta tatcattttt
      781 atttacaata atgcttttgg tccttatttt tttatctatg atatttttag tttacgggga
      841 aaaaataggt tattttattt ttaatcttgt aggattggat gagatattta taaaaatatg
      901 ggatattcta agatacactg taggtataat aacaataata gtgatattta cacttttata
      961 taaatacact ccaaataaaa agttaactat taaagaatct gcgccaggtg ctatttttgc
     1021 tacttttgct tggtttttag tatcattttt atattcttat tatacaaatt actatgctaa
     1081 ctatgaagta atctatggaa gtatagctga aataattgta cttatgacct ggatgtattt
     1141 tagtagttgg tctatcgtta ttggctatga ggtaaattca agattgtatt ttagaaagat
     1201 aagacatgaa atgttaaagt aaagtattta aaataaaaga gagtatttag tattataaat
     1261 gtaattgaat taataaagaa atattgacat ttaagttaaa aagtctagaa tttttctggg
     1321 ctttttttat ttttagtata acttgacaaa ttttatttaa tgggatagtt ttaatttata
     1381 atcatcttta aaaagatgac tattgataag taagagataa aattggtaag tatcaaaaaa
     1441 tatactaaat aaaaattata gtcctaatga gttaatttct gtattttcaa ataatgcagt
     1501 atttagtcat gtgtttttat atgtgcctgt atttctaatt accaatatat tagaataagc
     1561 tactgtaatt agatttagaa aaaagcgaca taaataatta taaacaaata ataaattaca
     1621 taaaattaaa tttattcaca agtatatctt ataaaatatg ttataattaa tagaaaattg
     1681 tcatataatt aattaagtag tattattata aataacattg aaaggtagtg gttagaatat
     1741 ggatacccat aataaatatg taaattttat aaaaaatata cctgtacctt ttttatattg
     1801 tagaattgta aagagacaag aagatataga atatcgagta gaatatataa gtaagggtat
     1861 gggcaaagta ttacaattag aagaaggtat ttgtgataaa aatatactag atgtattgcc
     1921 tgtatttaag tctaaaaaat atttcaaaga gttgttctca aatgaagtgg attgtataaa
     1981 aaggtacatt ccgacactta aaaactggat aaatataaaa aaacagataa taggggattc
     2041 atacataatt ttgtattttg gtaaaattgt atttgattat agacagataa tcgattcctt
     2101 tgataaaaag gaaaaagtag cttatattaa agacgaagaa ggcatatata tagattgcag
     2161 tgaaaattta ataccaatat taaataacaa cattaaaaca acgaaagata tctttggtaa
     2221 aaatgatata gaagtatggg gagagaatac aggaaaatta tttagagatg attatagaga
     2281 aggagtatct agtaaaaaaa gatttttaca aaatcttttt gaatatgaag aaacattttt
     2341 tatggttgaa aaatattttt tgtatgatga agacgaactt ttaggtacta tagggatagt
     2401 agacaatata atatatagtg gttacagtaa tagaaattat aactcaaaag atttaatgaa
     2461 gatgatagaa cactcaattc cagagaatat gttttataaa gatgtgtatg gaaattatat
     2521 tggatttaat tcaggttttt tgaatttagc ttgtatgaat aaagaagaat tattagggaa
     2581 gaatagttat aaaatatcag aagaagaagc tttaatagat aagatatttg aaagtgataa
     2641 gggtgtagtt gaaaataaaa aagtagttac atttgagtta aatataagta tgaatgatga
     2701 aaacaagtgc attgagatta caaaaaggcc gttttttgat agctatggaa gtgttattgg
     2761 aataattgga acagcaaggg atataagtag aagaaaaaga ttagaagagg aaatggataa
     2821 aactaggatg gaattttttg caaatttatc ccatgaactt agaacgccta taaacttaat
     2881 atcatcttca ttacaagtga tagaaaaaaa agaagcagat ttaatagaat caaatgatac
     2941 tttaaaaaga aatttaggta taataaaaca aaatggaaat agaatactga ggttagtaaa
     3001 taatgttata gattttacaa aaatgcaatc aggatattta gattttaaac cagaagaatc
     3061 tgatataatt gcatttatag aggagatatg tatgtctgta gctgattttg caagccaaaa
     3121 taatatacaa cttacatttg atacagaaat agaagaattt tcaatgttat ttgattcaga
     3181 aaaattggaa agaataatat taaatttact ttctaatgga attaagtaca ataagaaaga
     3241 tggaaaaata aatatatttt tatatgtgaa agataatgta tttaatatga aaatatctga
     3301 tagtggtata ggtataccaa aagaaaaaat agataaaata tttaataggt ttgaacagat
     3361 tgacaatgaa ctttcttaca gagttaaagg aagtggtatt ggactttcat tagtgaaatc
     3421 tttggttgag ttgcatgagg gaagtatatc tttaaaaagt caacttggta ttggaagtga
     3481 atttatagtt tcattgcctg ttagaagtaa aaataatatt gaaaaatata atcataaaag
     3541 agaaatcagc aatgaactaa gtaaaaaatt agaaatagag ttttcggatt tataagttta
     3601 aatataaaaa ataaaatttt aaaaaagtat tgacaattga tttagaaggg tttaaaataa
     3661 attacaacat aataaataaa aaggcgatga ataggaagag taaatctaat tcgattacca
     3721 gagagaagag tccgttggct ggaagacttt ttaaattaga gggattgaaa actacctagg
     3781 agctactttc tgaagtcatc tgcattagta gattttagta agaatagtcg ttagctgcgt
     3841 taaagtgtaa agtgagtttt catgacatat gaaaactaaa ttaggtggaa ccgcgggaat
     3901 aatactctcg tccttatgtt ataaggatga gagttttttg ttatttaaaa atataaacag
     3961 taatcataat aattatagaa aatataaaag caatgaatag gaagagtaaa tttaattcga
     4021 ctatcagaga ggaaagttca caggctgaaa gactttctaa gtttgagaaa ttgaaaacta
     4081 cctaggagct gtggtctgaa gttactttaa aaagttttag taagactaac cgttaactgc
     4141 gttaaagtgt aaagtggatt ttatcaataa atgataaaat caaattgggt ggaaccgcga
     4201 gtatagactc gtccctttat atagggatga gtctattttt attgcaataa aagattgaaa
     4261 ataaaaaaaa atcggaaaat tataaattta ggaggaaaat aaaatggaga ataaagtgaa
     4321 aattggagta ctaggatatg gagttgtagg aagtggactt atagatataa tagataataa
     4381 taaagaaaaa agaaatatag aaatagttgg aattttagtt aataatttgg aaaaacataa
     4441 ggataaaaaa tattctaata taataactaa taatatagat gatatattta ataaagatat
     4501 agatatactt gttgaagtca tgggtggttt agaaccatca ttatcttata taaaaaaagc
     4561 attaaacaat aaaattcatg ttgttacagc aaataaagat ttgttagctg aatgtggaga
     4621 tgagcttgca aaattagcaa gtgaaaataa agtaagtatt aaatttgaag cttcagttgc
     4681 aggaggtata ccagtattaa aaccaataat agaatcacta gaaggaaata atatagatag
     4741 tataaatgca attttaaatg gaactactaa ttttatatta tctaaaatgt atgatgagaa
     4801 tctatcttat gatatggcat taagacaagc acaagaatta ggttttgctg aagcaaatcc
     4861 agagtcagat gtattagggt atgatgctgc tagaaaacta tcaatattat caactctagc
     4921 ttatgacaat agagtatatt ggaaagatgt gtatttagaa ggaattacag atatagatga
     4981 aaaagatatt gaatatgcaa aaaaattaaa ttgtaaaata aaattgattg gtcaaagtaa
     5041 gtatgaaaat gataaagtaa gtgcttttgt tagacctgtt ttagtagaaa aagataatat
     5101 ccttgctaga atagacaatg agttcaatgc tgtcatagtg aatggagatt cagtaggaga
     5161 agtttctttt gtaggtaaag gagctggaag tttagcaaca ggaagtgcgg tttattcaga
     5221 tgttatagat ataatagata atagagtttc aagtatagat tcttttacta aagataaaat
     5281 acaagttaat aaaatagtta gagaaaagtg tggagcactt cttagattta aaaaatgtaa
     5341 taaagatgaa attctaaata tagttgagaa ttgtttagta aaattcgata ttttaaatga
     5401 tgatgatgaa cttgctatta tggtttatgc tgattctgaa tatgaaataa acaacagttt
     5461 atgtctaata aaagataaag gatactgcga aaaaatgaat aaaatgttga aaataagtta
     5521 aaaaagaaaa aatataagtt tatatgtaac atattttgac gattttaata ctatatagta
     5581 tgagatgaaa caaaacatct cataaaaaaa ttaaaattaa agggagagat atgttatatg
     5641 gaaaataaaa aatgttattc agaagattgg tatgaaagag gagaatctac agctaaatgg
     5701 ttccaaaatg atagagaaga atatgaaaga gaagcatatg atgaagatag agaaagaaga
     5761 ggttcaaact gtggatgttc agattcagga gaaaatagac ctagaaactg tgaaagattt
     5821 agaagagaag ctgagataag agaaagagaa gcaagagaag cattctgtga atcttcagag
     5881 aaaaagaaag aggcattagc atatgaatgt gaagctagaa aattatggga agaagcagaa
     5941 aaatactggg atgaatattc aaaatacaac tataaaggaa tcgaatattt agcagaagct
     6001 gctagattat ttgatgaagg tatggaatgt gaagctagaa gaaatggaaa taatggagga
     6061 aacaataata attgttgcca taaatgccat aaatgtaatt gtaactgctg tagaaaataa
     6121 aaaaattaat tgacaaatca atagttaaaa tatatcatat tatgtataat aaagagaaaa
     6181 ttcagaaaaa aataatgatt agaagaaata taagtatatt tattagacaa atagtgctca
     6241 gttagtgagg tgagtacaat atgattaaac tcccatcctc tagcttttga tagagggtgg
     6301 gagtttttat ttatttatat atcttaaatg aggataatat tgtaaaataa atgagtatga
     6361 gattgtaaaa ttaggaggta aaaaatgaaa agtattaaaa catcaagtat gaaagaaaag
     6421 gtagatgaaa agatattaag agagcaagta agttctataa tagaagatat aagaaataat
     6481 aaggatgttg cacttaaaaa atataatgag aagtttgata gaaatacaag agatgaattt
     6541 agaattacta aggaagaaat aaaggaagct tataagcatg tagatgacga atttataaat
     6601 aacttaaaga ttgctgccaa aaatataaga gaatttgcta aagctcaaaa actaagtttt
     6661 gaaaaccctt ttgaaaaaga aatttatcca ggtgtaatct taggtcaaac taatattcca
     6721 atagagtcat gtcttgcata tgttccagga ggtcagtatc cattgttttc aacagctctt
     6781 atgcttataa tacctgcaaa agtagcagga gtaaaaagaa tagtggcatg ttcaccaact
     6841 atgaaaaata ctgaaaagat aaatcctaag acattggtag ctatggatat agcaggtgct
     6901 gatgaaatat atgcaactgg aggagttcaa gctatagcag catttacata tggaacagaa
     6961 aagattaatc cagttgacat aattgtagga ccaggaaata agtttgtaac tgaagctaaa
     7021 agacaatgtt atggacaagt agggattgat tttgtcgctg ggccaagtga agttcttata
     7081 atagcggatg aaacttcaaa tccagtttat attgcagctg atttattagc acaatgtgag
     7141 catgatttaa atgcaagagg aatattattg acaaatagct tggaagtagc acaaaaagtt
     7201 gaaaaaaata tagaaagtat gctaaaagat ttgccaacaa aggacatagc atatagttca
     7261 tgggaaaata atggagagat aatattggtt gatgatatgg aagaagccat taaaataagt
     7321 aatttttatg caccagaaca tttagaagtt gcagtaaatg agtataatga tatttgtgat
     7381 agattaacta actatggttc tttatttatt ggaaatcttt ctgctgaggt atttggcgac
     7441 tatgtttcag gtaccaatca tacattacca acacttaaag catcaagata tactggtggt
     7501 gtttgggttg gaacttttat caaaacttgt acaaagcaga tatttaatga aaaagctata
     7561 caatcattag cacctgtagc tgaaaagctg gcaaaagaag agggattata tgcacatgca
     7621 aaggctgctg aggttaggtt taaaaagtag taaaaaattt tattttggca gtcgagattt
     7681 agtgttagac tgagataagg aaaacgctga gaacccgaat aaatactata ttttgtgggc
     7741 ctcggcgttt cttttatatc tgtatataat aggataatac tacctgtgtg tttaaatact
     7801 aacttaatat ttcttttcta aataatgttt atgaaatagt taatattttt agttaatagt
     7861 tgaaaaaaac ttttgctaac tgtgcaaatt catctgcatt tgtcaatata gcagggtgtc
     7921 ctccctgact aaaaatgtga atacttgctt ttgaaataaa agttgccatt tgtttgtact
     7981 cttcttcaag attatgacga cacatttcgt cttccttact tcctgtaaat aaaattggca
     8041 tttctaattt acacaattct ttatgaaaca aagggcgttt ctcgtttgca cattgcaata
     8101 gagcttttgt atcaagatta acaacattct cccaatcttt tccatgacac cattcataga
     8161 attttcgtgc ttgaatatct tgctttgatt tttcccttcc agtgattaga ttatctatga
     8221 agttatcatt aagagtacgt ccatcaaagc tatctgctat aattgcttca actaaatcgg
     8281 gacgttctaa tccagtatta acagctgccc atgctcctcc acttgttcca attaagccaa
     8341 ctttaccaca attcaaatgt tcaatcagtg ctattgtctg taaggcttca tcatgccata
     8401 tgtcaggaga aaattgagaa actctatccg actgtccatt acctaaaaaa tcaattaaga
     8461 tacatctaaa attttctgca tataatggca caagtgcttt aaacatatta gaagaaccag
     8521 tattaccatg caggaaaatt atcggtttac cttgtccata ttctttataa aaacaagaat
     8581 taccttgata ctgaaaatat gacatatatt ttcctccttt ttatttgata agttaattat
     8641 aacaatatag gtatgaatga aaaacctcat gttctttctt agaataaaaa tgtgccacgc
     8701 ataaaacgtg acacatttct aattataaat tttgctgttt aagtattctt tgtatgcttt
     8761 tctctgtcaa aaagtatttc tctgataact gcttgatact cattccattt tggaaattta
     8821 tacagatttg agaattacga gaagctaaga cttgtttggt gtttgtgttt tcaccccaat
     8881 gctttttgtg ctcttgcttt ttgggaatat aaatcacttt accatctata tattcttgca
     8941 cttgctcaat cagttcttta ggcagtaagt caatcgaatt ttgatagctc attttgcacc
     9001 tcctaaataa ttattcttta ggattagcaa aggctactgc cactcacaat taaaaataaa
     9061 attttttatt atgtacaata gcctttgcta tgcacaagtt ccaattccca taaactaaat
     9121 actcctttct ggtttgaaaa attcatctga acattgattt cgcttttaag atagagttca
     9181 atgacaaatt tacattttta ttatatatat tcagatagat aattgcaact gtctttgtat
     9241 gaggatattt tattatagtc aatactataa tatgtattta tatcatcaaa ctaatatgaa
     9301 cagttgaaag gaatatgaac aataaaactt agtcaggaag aataattatg aagcagataa
     9361 tagagaaatt tgattttaaa gttttattga aaatttattg acaaa
//
LOCUS       DAKBNN010000151         9205 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000151 DAKBNN010000000
VERSION     DAKBNN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.152"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(261..404)
                     /gene="scfA"
                     /locus_tag="OUB02_002883"
     CDS             complement(261..404)
                     /gene="scfA"
                     /locus_tag="OUB02_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HCT8886622.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(470..832)
                     /locus_tag="OUB02_002884"
     CDS             complement(470..832)
                     /locus_tag="OUB02_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HCT8886623.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(890..1183)
                     /gene="yajC"
                     /locus_tag="OUB02_002885"
     CDS             complement(890..1183)
                     /gene="yajC"
                     /locus_tag="OUB02_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCT8886624.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1317..2438)
                     /gene="tgt"
                     /locus_tag="OUB02_002886"
     CDS             complement(1317..2438)
                     /gene="tgt"
                     /locus_tag="OUB02_002886"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCT8886625.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2517..3002)
                     /locus_tag="OUB02_002887"
     CDS             complement(2517..3002)
                     /locus_tag="OUB02_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886626.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3014..4039)
                     /gene="queA"
                     /locus_tag="OUB02_002888"
     CDS             complement(3014..4039)
                     /gene="queA"
                     /locus_tag="OUB02_002888"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCT8886627.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4357..5376)
                     /gene="ruvB"
                     /locus_tag="OUB02_002889"
     CDS             complement(4357..5376)
                     /gene="ruvB"
                     /locus_tag="OUB02_002889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCT8886628.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5483..6085)
                     /gene="ruvA"
                     /locus_tag="OUB02_002890"
     CDS             complement(5483..6085)
                     /gene="ruvA"
                     /locus_tag="OUB02_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCT8886629.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6078..6587)
                     /gene="ruvC"
                     /locus_tag="OUB02_002891"
     CDS             complement(6078..6587)
                     /gene="ruvC"
                     /locus_tag="OUB02_002891"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCT8886630.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6915..7544)
                     /locus_tag="OUB02_002892"
     CDS             complement(6915..7544)
                     /locus_tag="OUB02_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886631.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGTKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7747..8070
                     /locus_tag="OUB02_002893"
     CDS             7747..8070
                     /locus_tag="OUB02_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HCT8886632.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8338..9192
                     /locus_tag="OUB02_002894"
     CDS             8338..9192
                     /locus_tag="OUB02_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886633.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
BASE COUNT         2970 a         1602 c         1044 g         3589 t
ORIGIN      
        1 cataaattag agtttaaaat taaaaattat tctaatacat gtaattgata aaaaataaaa
       61 tataagagta gctactttaa aataaatgtt cttatatgat tagatatttt tgcataatta
      121 aacgataaga aatcaaattt aattttacat aaatagaata tatattttta tataataatt
      181 gaaaataaaa gaggatacct cataatgata aaagaatcac tttgagatac cctcttacaa
      241 acaactaaat caataatttt ctatctttca cactcttgat ttgcaactgt acaagaagtt
      301 ttacaagctg attgacaaga agtttgacac tctccacatc caccttttgc agcactttgc
      361 tttaaagtag cttgagataa agtttttaca tgtttatttt ccattttcaa cgcctccatt
      421 tctaattgtt tcctattgat tatatcataa aactacttta atataaactc tatttaaagt
      481 tgacacccaa tacgcctcca atgcctcctg ctgctgatat tagcaataac ttataaatca
      541 tccctatttc aaatacaaac ttttcttgtg ctaaaaacac agtcaaaaaa atacatacta
      601 tgtacaaaag tcctactaaa agcccataca taagtccttt ttctttcatg tgttttgaag
      661 tgtaaaaccc tcctatagct gcagaaatag tagttataaa tgatgagacc atagttatat
      721 tatctccacc aatatctgta aatgtcaaaa ataagttata cactaataaa acagccaaag
      781 ttattatata tgcatatcct agacctttaa aaatataatt agattttccc ataaaaaaac
      841 ctcctctcaa attaatatat attcgagaaa aggcttttaa attactaatt tagtcttctt
      901 ctatttcttc ttttttagat ttaacagttc ctatagccca tttttcaaat ggaactttag
      961 ttctatttgg tcctaattct aatattacaa catcatcttc aactttagct acattagcta
     1021 ctattccacc tattgtaatg accttgtctc ctacacttag gctacttctc atttctttta
     1081 attgtttgtc ttttttcttt tgaggtctaa tcattaggaa ataaaagatt gcgattacta
     1141 ctacccatag acctatactc ataattattt gttgctgtgg cattaatttt tcactccttt
     1201 gtaaattata ctcttttgta aatcataata aatgtttata taacaattta ctttctctat
     1261 ctttgagtac tattgaatat tatatattaa aatgattata attaatagtg caaaacctat
     1321 atttcatatc catattttgc aaaaaattca tttctaaaat caagtaatct atcttccatt
     1381 atcgcttgtc ttatttgttt cattaaattt aataaaaagt gaagattatg agtagttatt
     1441 aatcttgcac ccagtatttc atttgctttt ataaggtgtc ttatataagc tcttgaatag
     1501 tttttacaag cataacaatc acattcaggg tcaagtggag taaagtcttc agcataagtt
     1561 gcatttctaa ctactacttt accttggcta gtcattgcag ttccatttct agcgatacga
     1621 gtaggtagga cacaatcaaa catgtctact cctcttataa caccttctac taagtcatct
     1681 ggactaccaa ctcccattag gtatcttggt ttatcctttg gcataagtgg agttgtatgc
     1741 tctaacacgt catacataag tggttttggt tctccaacac taagtccacc aatagcatat
     1801 cctggtaaat caatattagt tatttctttc gctgattgtt cccttaagtc tttatacatc
     1861 ccaccttgta ttataccaaa taaagcttgc ttgtcggtat tattatgggc atctttacat
     1921 ctttttaacc atcttgttgt tctttctaaa gaatttttta catattctct atctgatggg
     1981 tatggtgcac actcatcaaa tgccatcatt atgtctgaac ccaatgcatt ttgtatttcc
     2041 atagcttttt ctggagttag aaaatgtttt gaaccatcta agtgtgacct aaattctaca
     2101 ccttcttctt ttatctttct taaaggccct agactaaata cctggaaccc tccactatct
     2161 gtaagtattg gtttatccca attcataaac ttatgaagac caccagctct ttttataagt
     2221 tcatgacctg gtctcatata taaatggtat gtgttactaa gtattatttg tgaaccaatc
     2281 tcttttaatt cttctggtgt catagatttt acagttgctt gagttccaac tggcatgaat
     2341 attggtgttt caataatccc atgtggagtg tgtaatcttc ctaaccttgc accactctgc
     2401 ttacaagtct ttatcaattc gtatcttact gcgtacataa agttctcctt aatcatattt
     2461 atttctcatt atttcttaag atatatttgt aataagctat tcatataatt tattgtctaa
     2521 atagatttat cttcgtcttc tttgttctgt tgttccttaa ttgcttcttc atcatgaata
     2581 ctcattatat taagagcact cttgtctttt gttactaaaa acttttttat agcaaatagt
     2641 cctgctatag ctataactcc tattaaaaca tctatcatac cttcattttt aggcagtagc
     2701 aacatttttc tagctattgc gtaaagtaat gcctcaataa atgctcctgg tatatgaaga
     2761 gatagcatta ctacaagttc aacacctatt actaaaagta atatttgtgc taaaaaatca
     2821 ttaagctgac tgtattgtac tggattttga aaatcaacta tatatgctcc ccacataagt
     2881 cttagaacat ctatagttcc taaaaatact gctattagaa ctactactgc taaaacacgt
     2941 tcaaatataa aagcaatctt taatataggg ttattctttt tttgtttttg taaactatgc
     3001 atccattacc tccctacttt ataatcatag catctccaaa actgaagaat ctatatcttt
     3061 cttttactgc ctcattatat gcatttaaaa cattatcctt tccacaaata gcacttacta
     3121 acattattag tgtagactct ggtaagtgaa aattagtaat aagtttatct aaaactttaa
     3181 atttataacc tggatatata aatatttctg tccatccact agtttctttc atctttccat
     3241 cctcattaca agctgattct atagttctac aactggtagt accaacacat attacatttt
     3301 ttccattttc ctttgctcta tttattttgt cagctgcttc ttggctaacc atataatatt
     3361 ctgaatgcat cttgtgattt aaaacatcct ctacttttac tggtctaaaa gttcctaaac
     3421 caacatgtag agttacaaag gctatatcta cacctttttc ttttatttta tttaacaatt
     3481 cttctgtaaa atgtaatcct gctgtaggtg ctgctgctga accattatgc tttgaataaa
     3541 cagtctggta tctttctttt tcatctagtc tagcagtgat atatggaggt agtggcatat
     3601 ttccaagttc atccaatatt tcttcaaaaa ttccatcata atggaatttt ataattcttg
     3661 aaccttcatc tgataaatct acaacttctc caattagctt tccatttcca aaagaaaatt
     3721 tagtccctac ttttgctctt tttcctggtt ttactaaagc ctgccaagta tcttcctctg
     3781 ttctttttaa taataaaaac tctattttac ctccagtttc aagcttctct cctattaatc
     3841 tagctggtat aactctagta ttatttaata ctaagcaatc tcctggattt aaatattcta
     3901 ttatatctct aaatacttta tgttcaatat tacctgtctc tttatctaat accatcaatc
     3961 ttgagctagc tctatcttct ataggaactt gagctataag ttcctgtggt aagtcaaatt
     4021 taaaatcact tgttttcaat gttaaacttc ctctcttaaa atttctccag ctacaataat
     4081 atcaaaaaaa caacttattt tccacaatat taccataatc tatcaagaaa ttgctatttt
     4141 taacagacac catatccaca atatatagat atttttccac gatatacatg tcttatccac
     4201 aatatataga tacaatacac acaattttta tcgtgtttat ccacagtatc tacagatata
     4261 attaccattt tgtggatatg tttgaatttt tagccatttt agaaatattt taatttaatg
     4321 actcttttat tttataaata ctcgtataat ctatttttat ttttcattag gatatggaat
     4381 ttttaagtgt tcatatgcta atggcatggc tactcttcct ctaggacctc tatttataaa
     4441 tcctaattga agcaaatatg gctcatatac atcttctatt gtatttctat cttctccgat
     4501 agaagctgcc aatgtatcta aacccactgg tcctcctcta aatttttcta ttatagtcat
     4561 aagaagcttc tcatctacaa aatcgagacc caaactatcg actcctaaaa gttccagagc
     4621 atcttttgcc accttatcag ttatttttcc atcagctcta acttgtgcaa aatctctaac
     4681 tctttttagt aatctatttg ctatccttgg agtccctctt gaacgttttg caagttctaa
     4741 tgctccatta gactgtatct ctgcatctaa gatgcttgaa gacctaagta ctatttttga
     4801 aagttcatca actgtataat agtccaattt acaaataaca ccaaatctat ctctaagtgg
     4861 atttgtaagc ataccagctc ttgtagtagc tccaataagt gtaaatttag gaaggtcaag
     4921 tcttatgctt ctagcagatg gccctttccc aattattata tctaggcaaa aatcctccat
     4981 tgctggatat aaaacttctt caacacttct attgattcta tgaatctcat ctataaataa
     5041 aacatcattt tcatttaaat tcgtaagtat ggctgccaaa tctccagctc tttctattgc
     5101 gggtcctgac gtaattctta aatttacccc catctcattt gctattatac ttgctagtgt
     5161 agtttttcca agacctggtg gtccatataa aagcacatgg tctaactgct catttctcga
     5221 tttagctgct tctataaata tactaagttg ttctttggat ttttcttgac ccaaataatc
     5281 ttctaaagtt tttggtctta aactgttttc tatatctata tcttccatct tcattgtaga
     5341 agttattatc ctattttcat cttcaaaacc ttgcatttat acccctcctc tcatactttt
     5401 gaaaatctac tttatatatt ttcaaattta cataaactcc ttaaatattg ttttaaaatc
     5461 caaatattat aaaatccaaa tactatttca aagatttact cattatatat gtaagtgctt
     5521 tctttataat agcttctgta ttcataccat cttttttaca tttttgaact gcctcttttg
     5581 attccgacaa agtatagcct aaagctacaa gtgcatctac agactcatct tgtgatatca
     5641 aggtaggttt ttgtgataat aacgttggtt caaactctat gttatttttg tctaccttat
     5701 ccttaagctc taagacgatt ctttctgctg tctttttacc aacaccagga gctttggata
     5761 attttccaat gtcttcactt aaaatatatg ctcctaactg tgctggtgat gcaaatgata
     5821 gtattgaaag cgccacctta ggtcctattt tagatacaga tgttagtaat tcaaacatct
     5881 taagttcttc cctacttaca aaaccacaaa gactcatatc atcttctctt acaattaatt
     5941 tagtatatat ttttgtggct tctcccactt gtaaattcat tattgtattg gatgatacat
     6001 ttattttata tcctatccca ttattttcaa caactatact gtctatataa atttcttcta
     6061 ctgtaccttt tatataacta tacatactta ccacctatat tctttagagt tttttcaagt
     6121 ttgtttgaat gagcatggca aatagccaca gctaaagcat ctgcaacatc atctggtttt
     6181 ggaacttttt ttagacttaa aaatgaagtt accatctgtt gaacttgtgc cttatctgct
     6241 ctcccatatc caactacacc ttgttttact tgaagtggag tgtattcata tacaggcttc
     6301 ccattatgag cacatgcaag catagtaaca cctctagctt gtgcaactgt tatagctgtt
     6361 tttacattct tgttaaagaa taattcttcc attccaactt catctatatt gtaattcttt
     6421 actattgtat caattccttt atacacaagt tccaatcttc tcgatatatt catatgggca
     6481 ggtgttgtaa ctgctccata atcgattact ttaaacttgc tatttttgta ttcaattata
     6541 ccatatccaa ctatggctat acctgggtca atccccaata ttatcatttt ttaaaattcc
     6601 ccctaaaaat aaaagaaata ccaaaatgta tttctcataa aaacttatgt tctaaatcat
     6661 tatatcataa ataatattaa caaatcataa aatgctctat gtaaaatata aaatcaaata
     6721 aaaaagttat atcaaagcaa gattttacct caatataact tttatattta ttacaaataa
     6781 attattgtat ttttatagga attaaatgtg actacaattt taatactaat ctatttaata
     6841 ttaaattatt tattattaat ttacttagta tttaacttac ttagtattta acttaaaata
     6901 atattaattt actattaact tgcaaagtct tctaatcttg aataggcctc ttcttcagta
     6961 tccataacta cacctttttt gatttcatct tgaactgttt taggaagtga atctactctt
     7021 atttttgttt tttctactaa tgttttagtt ccactatcat catatttata tagtgctata
     7081 tttccattat cattttcaat agtatattta ccttttatag taacatccat tgcttgtaca
     7141 tctttattat cactatattt agattctacc aatatgattt catttttatc catactcttt
     7201 atttttttag ttggatattc ttttttaaaa tatgatacta tttctgactt cgtcttatct
     7261 aataatgact ctggaactat tcctgccatt gtaagtgttc tcttatagtt agtttcattt
     7321 tcgccttcac tttgaagcca aacttcacaa tttttattta atccaaaagc ttcttttgac
     7381 ttttcattca tattcgcact agtacttaca acttcaccag tttgaactcc attgtgtttt
     7441 aatcttgtcc ctatataaac tccgctactt aatacaaata ctccaaccag tattaaggca
     7501 gatattttcc aaggaaataa ttcttttttc ttcttttcaa acattgatta cgcctccttt
     7561 ttctattatt ttctccaagc tgtaagtttt tatacaattt aggtaaattt tagaagaaaa
     7621 tatatttata tttcaaatta ctctaagaat agatattttc taaaaggtag ttttctctta
     7681 atggttatat tcttgtaatt atatggacat aatttataat aaataatcgt ttaggaggta
     7741 ataactttgg aaagaataaa ctgtaatgta attaactgtt cacataacaa cagccatata
     7801 tgttatgcag ataaaatttt tataaatggt gaaaaagctc acaatgatag acatacttgt
     7861 tgtagttcat ttttagatga atctatttat agtaatttat ctagcaacgt taataataga
     7921 aattgtgagg aattagtttg tactgtaaaa aattgtaaat acaacaaaga tggtagctta
     7981 tgtacacttg ataatatatt ggttgctcct gaaactgata gagtaaatct ttatattgaa
     8041 acatgttgct ctagcttcga atgtaaatag tcatagtata aatataaggg tgtcaaatgt
     8101 aattaaacta acatagtgta gagtatctac aactattagt ttgattatac ttgacaccct
     8161 tatttatttt aaataaaatt tcattattat aaccatgttt ataatgttct aactgtaatt
     8221 aaatttgata aatttctata aattggtata atcaatatgt aaattgaatt tagtaaatta
     8281 atcataaaaa taaaatatcg agtatatagt gttatactaa ctagaaatga ggtgcttatg
     8341 agtacatttt atattcaatt tctttatttt gtaatttata gctttatagg atggtgttgt
     8401 gaaactacat attgttcagt tttacaaaaa caatttgtaa atagaggttt tttaaatggt
     8461 ccattttgcc ctatatatgg atttggagca ttagcaatag ttgctacctt gactcctttt
     8521 gtacacaata ttcctttgtt atttttgttt agtataatta taactagcat aatggagtat
     8581 tgtacgagct ttattttaga aaaaatattt aatatgactt ggtgggatta ttctaaacat
     8641 aaatttaata ttcatggaag agtttgtctg gaaaactcac ttatgtttgg tgtattatca
     8701 ttgattgtaa tgcttatagt acatcctatt gtggttgact ttattaactc tatctccaaa
     8761 aatgtgctat ttatatttgc tatatctata gaaatttatt ttgttttaga tttagttatc
     8821 acagtccata caattttaca attaaatggt aagttaaaac aaatcaatct tataattaag
     8881 gaattaaaag ataagaaaga atactacaaa cttattacac aagaaactat agaaaacaaa
     8941 cttgaatctt tagttgaaaa tagactggat actttggaag ataggtttga aatacttaaa
     9001 gaaaaagaaa attatcgcta tgctaaaaat cgtattgatg agctaaaaaa taaacttaat
     9061 actttgctgt ctaaccacaa attacttcat agacgtataa tcaaagcatt ccccaacata
     9121 agctctaata agcatacaga tattttaaat aaaatcaaag aaaatattaa atcaattaaa
     9181 aaagataaat aataaaataa acaca
//
LOCUS       DAKBNN010000152         9058 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000152 DAKBNN010000000
VERSION     DAKBNN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.153"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            62..1123
                     /locus_tag="OUB02_002895"
     CDS             62..1123
                     /locus_tag="OUB02_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCT8886634.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1278..1790)
                     /locus_tag="OUB02_002896"
     CDS             complement(1278..1790)
                     /locus_tag="OUB02_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCT8886635.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            2310..3245
                     /locus_tag="OUB02_002897"
     CDS             2310..3245
                     /locus_tag="OUB02_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCT8886636.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(3337..5208)
                     /locus_tag="OUB02_002898"
     CDS             complement(3337..5208)
                     /locus_tag="OUB02_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCT8886637.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            5398..6324
                     /locus_tag="OUB02_002899"
     CDS             5398..6324
                     /locus_tag="OUB02_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCT8886638.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDEHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            6463..6609
                     /locus_tag="OUB02_002900"
     CDS             6463..6609
                     /locus_tag="OUB02_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886639.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(6714..7238)
                     /locus_tag="OUB02_002901"
     CDS             complement(6714..7238)
                     /locus_tag="OUB02_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8886640.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGGKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7291..7515)
                     /locus_tag="OUB02_002902"
     CDS             complement(7291..7515)
                     /locus_tag="OUB02_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886641.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7712..8836
                     /locus_tag="OUB02_002903"
                     /pseudo
     CDS             7712..8836
                     /locus_tag="OUB02_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
BASE COUNT         3109 a         1353 c         1068 g         3528 t
ORIGIN      
        1 actaggtaat aatattaagt attataatct agttctttaa acatgtttac attgaggagg
       61 gattatcata aaagagttaa taattagaga tttagttgca aaagttccta taattcaagg
      121 tggaatgggc gttggtatat ctaggtcatc actagctggt gcagttgcta agcttggtgg
      181 tgttggtgta atttctggag ttcaaattgg atatgatgag gaagattttg aaactaatac
      241 tataaatgca aatttaagag ctataaaaaa acatatttct aaggccaaag aaatctcaaa
      301 tggtggtatt attggtatta attttatggt agctatgaaa gaatatgaaa catacgtaaa
      361 ggaagctgtt aaagctggtg tagaccttat aatatctgga gctggattac ctaataagct
      421 accatcatta gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa ctgcaaaagc
      481 tgctaatgta attttaaaaa tgtgggatag aaaagagaaa actactgctg acttaatagt
      541 tgttgaaggt ccaaaagctg gtggtcacct tggatactct aacgaggaac ttgataacat
      601 agattctata gactatgata aggaatttgt tgaaatttta aaggttgcaa atacttatgg
      661 agaaaaattt ggtagaaaca taccagtagt tgctgctggt ggtataactt ctagtagtga
      721 tgttaaaaaa tatatagata tgggtgcaag tggtgtacaa gttggtacaa gatttgttgc
      781 tacttacgaa tgtgatgccc atgagaactt taaaatggca tacataaatg ctagtgaaga
      841 agatgttcat atagttaaaa gtcctgtagg tcttcctgga cgcgccataa gaaacaaatt
      901 tatagaagaa gttaaaataa atcgtccaga aataaagaag tgctataatt gtttgattcc
      961 ttgtaatcca aaagaaactc cttactgtat ttctcaagct ctaatcaatg cagttaaagg
     1021 agatgttgaa aattcacttt tattttgtgg aaatgatgcc tataaaattg ataaattaag
     1081 tactgttgaa gatgttatca atgaattgat ttctgaatta taatttaaag aaaactaaat
     1141 taagatttta tcaagctaag gagtttaaat acgttattta ttaatcttta taaacatatt
     1201 tatgtcctta gcttttttgt gtcaatttac ttttatggaa attttaattt aattagaaat
     1261 agtattaacc tttataacta atcttctttt acaagtacat tggttatttc agctatatat
     1321 aaagtatgat aatctttttg tggataccat cttccatcta taccttcagc aataaaacat
     1381 tgaggttcaa gtttctgact atacattttt ttgcatataa tagccatttt agattcttta
     1441 aaataaggtg tatcatttaa gtgttctata ttaaatccaa tctgtgatat cttatcctct
     1501 tctctaccag atacttttcc acaatatgaa agctctttac gaaaatcttc atcaaaaaat
     1561 gtaagagaaa atttatctgt attatctata aattccttag tataacgctg tggtcttatt
     1621 actacaaaag caacatcttt tccccacata actccaagcc ctccccagct agcagtcatt
     1681 gtattcactt taccttcatt ttctgctgtt ataagtgtcc aatctttacc tatcaatcca
     1741 aatggattat ttttaatctc ttctgccaaa atttctttaa aatcactcat atctctaata
     1801 acctctcatt ctcttatatt tatattcctt ttattttcgt gataaattat aaatgaagtc
     1861 tagttttgaa aataaaatac ttttttaaat atattattgc aaataagtaa atatatatat
     1921 acactcttat tttttaatat atttaataat ttcactcaga atatatatat tctttactca
     1981 aattaaatat aacttatata ctttatgtgt aaattgtggt aaaaatatta tttaatcaaa
     2041 tcaaaacttg atttatatac ctggaaaagg tattatatta aagagtgctt aaaatacgca
     2101 tttcataaat agctatatat aaaataataa ataaggagag tgatgggcaa tggatactag
     2161 tccgttgcgg agagaatttt ttacattttc atcacttaat aatatctaaa atcaaaacta
     2221 atacatatat tcagttatat aaattagtta attaatagat actattaaat gatgagattt
     2281 atataaaact gaataaagga tgtgttgcca tgataagata ttacaaaact atagattcaa
     2341 aattagagaa acttagtttt tttgaagatg gctgttggat aaatcttgtt gaacctaatc
     2401 atagtgaaat caacgaaatt tctaatttat taaatataga tgtagaaagc atagaatctg
     2461 cactagatga agaagaacgc tcaagaatag atgttgaaga caatcatact cttatactta
     2521 tagatatacc agtagacgaa agtgactcta attcttctca ttatactaca atacccttag
     2581 gtattatact aactgaagaa gctattgtaa cagtttgtga tgcacaaact aagattttaa
     2641 atgactttat agttggtcat attaaagatt tttttacgtt taaaaagact cgctttttgc
     2701 ttcaaattct tcacaaaaat gcagcttatt acttgcatta cttaagaaaa ataaataaaa
     2761 tgacaatcat aatagagcga gaaatttaca aatcaatgaa aaacaaagaa ttggttcaat
     2821 tattggaatt agaaaaatct ttggtatact tctctacctc actaaaatct aatgaacttg
     2881 ttttaaacaa aatggttaga actgctggta taaagaagta tccagatgat gaagacctat
     2941 tagaagatgt tatagttgaa aatagacagg ctcttgatat ggctaaaata tatggagata
     3001 tattgagtag aattatggat gctttcagtg caattataag taataatcag aataatgtta
     3061 tgcaaatttt aacagtagtt acactaattt tctcaatacc tacaataata tcaggattct
     3121 ttggtatgaa tgtcatcaac atgccatttt ctaataaccc aaacggattt tggataatac
     3181 ttcttattac ctctataata tgtatagtga ttacattttt catgtcaaga aataagctat
     3241 tataaaatat taataaaaaa gccattagaa ttaattgatt tacatttatc ctaatggctt
     3301 ttatatattt aaatctatat aatcctataa tactttttaa ctatcatgtt cttcatctat
     3361 aatcatttca gtttcaagcc cttctagcat tgaatctaaa ctttctttta tatttagtat
     3421 ataaattggt acatactctt cttcatctat tacctcttct tgcaacttaa atggaggaat
     3481 taatacttca tcaaagtcta tatttaaatt aggaaagcct tctactatct cttcacaatt
     3541 atcgtactct tcatttgcta aagaaacgta catatctagg ctctttttat aataaagacc
     3601 tgcaagcata tactcacact tcttacttcc atatttaata ccttctttat aatatttctc
     3661 agcttctgcg tcatcattta attttaaata aatttgacct aaattatata ttgattcaac
     3721 acaaccttgt gctctagcct tttcatacca aaatttagaa tttacataat ctttatagaa
     3781 catatcataa attaatccta ccatatgttg agcatacaca ttattttcat tggcaggtgt
     3841 atcaaacatc attttagact cttccaattt accttctcta aaataaattc tacctagatg
     3901 tatacgagca ttcatatggt ttttgctact tgccatttca aaatactttc tagcattttt
     3961 tgtatttcct aagctctcat atatacaccc caatctataa ctagacctcg tatctccatt
     4021 ttcagatgct ttcttatacc aacttatggc ttcttcaata ttatttaaat cttcatagat
     4081 atttcctaat ttgatttgac aagatacatt atttggaatc ctcgaataat acgaaatcgc
     4141 tttttctata tcttgattct gataatataa atcccctaga ttttcaaggg aacttttgca
     4201 tccaacagct atagcatcct cataatattt aatagcattt tcataatctt tttcttcaaa
     4261 atatatacct gccagattat tttttgcctc tgttatatca tcttcagcag ccattttata
     4321 ataagttttt gccttctcta tctcattttc tctattatat aaattagcaa gtctatactt
     4381 agaaagtgta tgattttttt ctattgcttt ctcataataa taaattgcat ctcccagctc
     4441 ttcttttcta tcatatacat atgcaagcct atactcagac ttcacatcat ctctatcagc
     4501 actcttttta taatacttct ctgcatcttc taaattatca tttatttcca taatcatacc
     4561 caagttaaag caacctttca tatgacctga cttgtacaac tgtttatata aacttactgt
     4621 tgatttatta tctttctttt ttccatttaa tattgctaaa ttatattttg catcatcatc
     4681 taaaagcaca gaaccttttt tatagtattc atacgcttca tcatctttct ccaaaaaagt
     4741 atataatacc cctaaattaa aacaagcttt acctaaattc ttgtctgctg ctgtttttaa
     4801 ccatttttca caccttacaa aatctttcat tttaaaatgg catacaccta aattattttg
     4861 tgcttgtaaa taacctaaat ttgctatctt ttcataccaa tatatagcat attcatagcc
     4921 atctaactta tagtacatat tacctaaatc aaaagcagca tcttcacttc cacttttata
     4981 tgccattgaa taccactttt ctgcttcttt aaattgtttt ttactttcat atatatttcc
     5041 taaagtatat tgagatttta cgtaattatc caatgcagca ctcttataca actctattgc
     5101 gctttccatc ttacccatct tttcaaggag ggcaccctta gtatgtacga ctttcgggtc
     5161 attagatata ttttttattt tttttataga aaattttgta tttttcatta ttcgctacct
     5221 acccatttta taaaatatat tctctcaaac attattatac attataatct acaattaatg
     5281 ctataatatt gaatttttac cttttgtcac tatattgtaa cattaaattt taaattttat
     5341 aatcattaga ttttattatg taattggtat ataatgtata tatagggggg acattttatg
     5401 aatttcagca atatttttat ccaagttgca gttttattta taattatttt agttggatat
     5461 tttgtcagaa aattcaactt attagatgag cactgcacat ctaagctttc aactttgaca
     5521 atgacagttt ttttaccatc aatgataatt agctccatgc aaattaactt tgatagtaaa
     5581 atgatacaaa aaatactatt gttattattt acatctttga ttatgtatat agtttcaatt
     5641 ataattgcat ttttattgaa atacatttta aaatgtaatg ataaaaaaga tttgggaata
     5701 tatcagtata tagttgtttt ttcaaatgtt gcttttatgg gttatcctgt aattgaagca
     5761 gttttaggtc atgaagcaat attctatact gcaatattca atttgccttt taacttattt
     5821 tctttcactt taggaattta tcttttatct aaaggcagta caagtagagg tttttcaata
     5881 aaatcactta taagccctgc tacaatagct attgttattg gacttttttt atttgtaaca
     5941 ggacttaggt taccccaatt tataaacgag ccacttgaga tgttgggaag tataactaca
     6001 ccaatatcca tgattatcat tggaagctta cttgctaatt cttcagctct cgattgtttt
     6061 gtcaacaaaa aattatatat agtcactttt ataagattat tggtacttcc tgtgattgtg
     6121 tattttattc taaaaggatg gataaatgat aagatgattt tagctatacc agtagtaatt
     6181 tcttcaatgc ctgctgctgc aaatactgct ataatggcta atcaatatga ctccaatata
     6241 acactagctt cacaatgtgt gttttttaca acactatttt cagttatatc tatacctttt
     6301 ataagtattt tactcttatc ttgatttatt tttttatgta ttatataata acatattcaa
     6361 taaattacaa atataaacat tttattgtag tatattgatg gaattttttc ctagatattt
     6421 gtcaataata tctatatgta ttatagaata ggaggcttaa atatggatat ggatttactc
     6481 tttaaaatag tatttgctat agttgctata ggtgtttctg taaaaatcat taaaactgtt
     6541 gctagtttag cttttaaaat agcgttgata ttgctaataa ttttatttgt atatagaatg
     6601 tttttgtaaa catttaaaaa taagtttttt aacacaatag agaagtaaaa tagattatct
     6661 ttaatgtaaa ttttttatta aagacactct attttacttc tctattagct tctttaaatt
     6721 aaatttttat tatatcttcc cataatataa acatcttcaa attttccatc tctgattaca
     6781 gcatattttt tagttccctc tatttcaaaa cctagctttt tatataaatt taatcccttt
     6841 tcattatcca caattacttc taatcctact ctagttaaca taatccaatt atctgctata
     6901 tctaaaagtt gttccataag cttcttacct actcctcttc cctgataatt ctctgcaaca
     6961 cttattccta tttctgcgct atgtctcaat cgtggagaag aatttacatg taacccagca
     7021 aaacctatta catcttttcc tccattatta ttagtttcag ctacaaacat ataatcattt
     7081 tctcccaaag aagctaacat agcttcagtt ttatcgactc tgtcactcat taatgccaaa
     7141 gtatttctca taacactgta tgttcttcta atttcattaa aagcttgtgc atcttctctt
     7201 cttataggtc ttattgttat atctataggc ttactcatat tagattcccc ctcaaacata
     7261 atctttattt ttaattaaaa ttttacatct ttattttata ctgttcaaat ctatgtatgc
     7321 ttctacctga ggtacatata tcatattatc ttttatatgt gtacttccaa attcttttaa
     7381 ttctgtacct tctttatcct tagttatttt atatctaatg gattccatac cttcaactac
     7441 ttcagcatga aaaaacatac cttcatattg tttaaatcta aaattaaaat tgtctttagt
     7501 aagttttatt atcatatttt tatctactcc ttatataatt gatatttttc ttattattag
     7561 ttgtatatat actgcttatt catattaata tattataact tatcatttat ttagtataaa
     7621 gttataaata gatatattta tacttatttt acaatctttc ttcaattgat atataatatg
     7681 tttatataaa ctaatgtatg tgaggtgaaa tatgagcaaa aaaacaaaat atttttcttt
     7741 aattctcttt cctattgctt tgcttttaaa ttttatagcc agtaaagttc caaatattgt
     7801 tgaaaaatat tactcccaat tcatagataa aataattgta caaatattga gtaaagtttc
     7861 tggaattttc ccattttctc tctatgagat tacaatgtac ataatagtta tatctatctt
     7921 cttattttta tgttgtactt tatctacaat ttttaataag aaaaaaaaat taaacatata
     7981 tttaaaaaat tccattttaa atatattatc cattgtatca atagtctact ttctttttat
     8041 agtgctttgg ggaataaact ataataggat tccactagaa accacactta taaataacta
     8101 taatttaaaa aacaatacct ctatccaatc aaaacaatat agtgttaaag agcttactaa
     8161 attatataaa ttccttgtaa ttaaagcaaa tgaaactaga aaactcacat tacaagataa
     8221 aaatggtatt gtaaaatcta atacagatta taatggtgtc ataaatagag ctcaactagg
     8281 atacgataat atcttggata tattacctag tgttagtggt agttattcta agcctaagta
     8341 tattatttca tcaaatctaa tgtgctatac aggaataact ggtatatact ttccttttac
     8401 aggtgaggca aatgtaaata tagcaatacc tgacttatat attccttgta cagttgaaca
     8461 tgaaatggca catcaaaggg gatttgctag tgaagatgag gctaatttta tagcgtatct
     8521 tacttctata aaacacccca atattgattt taactattct ggatatattt tagctttaaa
     8581 ttatactgct tctgctttaa gtaaggttga ttataatgca tatgttgata tttctgctgg
     8641 catttcagat tctgtccgta gagattaaaa aaatgaaagt gaattttggc aaaaatatga
     8701 aggtaaaatt aatgaaattt ctaatgagtt taacaattct tacttaaaag ctaatggcgt
     8761 tactgaaggt actcagagtt atggtaaaat ggttgattta ttgctaactt attatgaatt
     8821 atatccatat aactaaacac tacaattcaa aattttattc atatacagct gatattaaat
     8881 cagctgtttt ctatttaaat aaattattta acttcaagca atcttatata atttgatagt
     8941 acttatacta tctgtataac atttcaattc taactatttt agttaaaaca tttctattat
     9001 cttttttaat aaatgtttta actaaaattt ttaaaacata aaacttccac attatttc
//
LOCUS       DAKBNN010000153         9021 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000153 DAKBNN010000000
VERSION     DAKBNN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.154"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            219..1820
                     /locus_tag="OUB02_002904"
     CDS             219..1820
                     /locus_tag="OUB02_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HCT8886642.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNLLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEDSL"
     gene            2039..3628
                     /locus_tag="OUB02_002905"
     CDS             2039..3628
                     /locus_tag="OUB02_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HCT8886643.1"
                     /translation="MKVNKRVLSIGLAISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVFKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDARELANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            3840..6110
                     /gene="slpA"
                     /locus_tag="OUB02_002906"
     CDS             3840..6110
                     /gene="slpA"
                     /locus_tag="OUB02_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HCT8886644.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFADDTKVETGDQGYTVVQSKYK
                     KAVEQLQKGILDGSITEIKVFFEGTLASTIKVGSELNAADASKLLFTQVDNKLDNLGD
                     GDYVDFLITSPGQGDKITTSKLVALKDLTGASADAIIAGTSSADGVVTNTGAASGSTE
                     TNSAGTKLAMSAIFDTAYTDSSETAVKITIKADMNDTKFGKAGETTYSTGLTFEDGST
                     EKIVKLGDSDIIDITKALKLTVVPGSKATVKFAEKTPSASVQPVITKLRIINAKEETI
                     DIDASSSKTAQDLAKKYVFNKTDLNTLYKVLNGDEADTNGLIEEVSGKYQVVLYPEGK
                     RVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDRIE
                     TAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSKDK
                     LDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAGDD
                     RYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATPIV
                     VVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQAT
                     NAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNSDG
                     KPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQVGK
                     GIATSVINKLKDLLSM"
     gene            6346..8691
                     /gene="secA"
                     /locus_tag="OUB02_002907"
     CDS             6346..8691
                     /gene="secA"
                     /locus_tag="OUB02_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCT8886645.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
BASE COUNT         3721 a          948 c         1704 g         2648 t
ORIGIN      
        1 aaagcagata tcttttatat atataaatat aaattcaatt ttgaatatgt ttaataaaga
       61 agttaaaaat aattttttaa atttaataaa ataaaacact aatatataga cgagatatat
      121 tcatatcata tatactctat aatatatgga aaataaagag aaaataaatg gaaaataatt
      181 acaatataaa tataaaatat aaaaatcatc agggggatat gaaaatgaac aaaaaaatat
      241 tatcattagg tctagcagta tcattaatct tagtaaactt caaaagtgta aatgcatcat
      301 cagtagtaga aaaaatatac ggtaaagata gatatgaaac agcagcaaag atagctgata
      361 aacagactta tgaaacagta attttagtaa atacagaaaa atcacttgca gacggattaa
      421 gtgcaagtgg attatcggga gctacaaaag ctccaatact atttacacaa caaaataaga
      481 taccagcaga cacaaataga tgtctaaaaa atatcaaaaa agcatatata ataggtacag
      541 aagatactat aagtaaatca gtagaaaaag aactagattc taaaaatata gaagtcaaaa
      601 gaattggtgg agaagataga ctaaaaacaa gctatttaat agctaaagaa atagcaacta
      661 taaaaaaagt ggataaagta ctattaacta atgcatatag tggagaagca gatgcaatga
      721 gtgtatcatc agtagctact agagatggag ctccaatcat acttacagat ggaaagagtg
      781 tgccttttga tgtaaaaaat attcaatcat attgtatagg ttcagaggag ataatgagta
      841 atcttctagt taaaaataca aactcagtaa gaatagaagg aactgaccga tttgaaacta
      901 acaaaaacgt aatagactac ttttttaata gtgcagatgg attctatgta tcagatggat
      961 accaattagt agatgcaata gcagccgcac cacttactaa aaactctcca atggtactag
     1021 taaatgatgg aagcgataaa actgtattag aaggagctaa gaatataacc tctgtaggtg
     1081 aaatagatga gaaggtaata caacaatgta taagtgcttc aaagtcaaat ggacaacccc
     1141 caacaattac agttggaagt acagaggtat ataagggtga aaagtttgac actggcaagt
     1201 taaatatagt agctaaagat aacactggga aggtattacc aatagaagtt gatgggttta
     1261 tagatacaaa tagagtaggt acatacatat tgacattaaa agctacagac gaatggggaa
     1321 aaagtacagg aaaaagagta gaaataaaag tgttggatga caaatctcat gattacaaca
     1381 gcccagaatt taaaaaaatg gtatctactg aaatgtataa cttaatcaat tcctatagaa
     1441 aagaaaaagg caaagagcct ttggtagtgt ctagtaggct agaaggtatg gcaaatgcat
     1501 ggtctaagta tatgatggat aaaaaagtat ttgcacatta tatagatgga aaaaatgctc
     1561 cacaagtatt ttctgaattt ggaatgagaa gtgaggaaaa tatagcatat atttatatag
     1621 attctaagaa cgttcaaacg acacaagatg caaaagactt ggcaaaggct atatttgaag
     1681 tgtggaagaa gtctccagaa tacaatgcaa acatgcttag tgatgagttt tatagtactg
     1741 gatttgggct ttatatatta tctgatggtc aagtgcatgc tactcaggag tttttaaatg
     1801 gaaatgaaga tagcttgtaa aaaattccat aaatcttgtt tttttgttaa tttactgata
     1861 ttataaaatg aaaatcacat ctaaagtact ttttgtaatc tgttttgtta ttgctttaca
     1921 ggttatggta ttttaggtgt tttttgtttt agtagtttat agattttgag ggaatcacta
     1981 ctgaggatgt aatttatgat tagctgaatt atataaaaaa attagtagga ggagtttgat
     2041 gaaggtaaat aagagagtat tatcaatagg attggcaata tcattaataa tggcaggagc
     2101 accaaatatc aatgcacttt caagtataga aaagatacaa gggaaagata gatatgagac
     2161 agctgccaaa attgcacaga aacaaacata tgaaaatgta gtattagtaa atacagataa
     2221 tacactagct gatggattaa gtgcaagtgg attagcagga acagttaaag cacctatatt
     2281 attatcacaa agaaacagta taccatcaga cacagaaaaa atgttaaaag atgtaaagaa
     2341 ggtttatata ataggaactg aagattcaat aggaaaatca gttgaaaatg aattaaagca
     2401 aaaagggata gaagttaaaa gaataggtgg taatgataga attgaaacaa gttatcttat
     2461 agcaaaagaa atagcttcaa taaaacctat agataaggtt tttataacta atggttatac
     2521 aggtgaagca gatgctatga gtgcgtcatc agtagcatca agagatggag cacctataat
     2581 tttaactaat ggtaaaaatg ttccatttga gaaaaaagaa ggtgtgcaat gctatgctct
     2641 tggttcagag gagataataa gtaatgattt agttaaaaaa actaatgcag ttagattagc
     2701 tggagaagat agatttgaga ctaataaaaa agttataaaa catttttata gttcagcaaa
     2761 agaattttat ctttctaaag gttatcaatt agtagatgca gttgctggtt cttctatagc
     2821 taaaaatgca cctattgtat tggttgatgg aaatagtgat aagtctgtac ttagaagtgc
     2881 tgataaaatc acagcactag gaggaataga tgaaaagaca ctagaacaat gtttaagtgc
     2941 atcatcactg gatgcaagtg caccaacaat aactgttgga aatttaaata tttatcaagg
     3001 tgataagttt gatataagta aacttaatat agtagcaaag gatagcaatg gtaacgattt
     3061 gacaccagag cttataggaa atatcaatac tgataaggtt ggtaaataca aagttactat
     3121 aaaagctact gatattggtg gaaagactac ctcaataagt gtagaagtaa atgtacttga
     3181 atataagact aatgatatga atagtagtga gtttaaaaga atggtttcta gtgaaatgta
     3241 taatttggta aattcatata gaaaagaaaa aggtaaagag ccattacaag tttttaaaaa
     3301 cttagaaggg ctttctaatt cctggtctaa gtacatggca gataaaggcc aattttcaca
     3361 tgtaatagat ggtaaaaaat ctgttgaagt atttactgga tatgggttga gaagtgaaga
     3421 aaatatagct tttgtaccac ttgttacaaa gagcacttat acaacaaaag atgctagaga
     3481 actagcaaat gtaatattta cagtttggaa aaagtcagat aaatataatg aaaatatgtt
     3541 aaatagtgat tttgtatata ctgggtttgg attatatata ttgcctaatg gagaagtgta
     3601 tgctactcaa gaatttttaa ataagtaaaa ttaaattttt taagttatta aatattgaaa
     3661 tttaagggta taaaaactca tatgcttggg aaaaataata atttcaaaaa aatgccttgc
     3721 aaaaagaata aaaatggatt attatagaga tgtgagaaat attaggaata tatggatgat
     3781 tattctatgt acataataag agatgtaatt ttaatataat gttgggagga atttaaggaa
     3841 tgaataagaa aaatatagca atagctatgt caggtttaac agttttagct tcggctgcac
     3901 ctgtatttgc agatgataca aaagttgaaa ctggtgatca aggatataca gtggtacaaa
     3961 gcaagtataa gaaagctgtt gaacaattac aaaaaggaat attagatgga agtataacag
     4021 aaattaaagt tttctttgag ggaactttag catctactat aaaagtaggt tctgagctta
     4081 atgcagcaga tgcaagtaaa ttattgttta cacaagtaga taataaacta gataatttag
     4141 gtgatggaga ttatgtagat ttcttaataa cttctccagg tcaaggggat aaaataacta
     4201 caagtaaact tgttgcattg aaagatttaa caggtgcttc agcagatgct ataattgctg
     4261 gaacatcttc agcagatggt gttgttacaa atactggagc tgctagtggt tctactgaga
     4321 caaattcagc aggaacaaaa cttgcaatgt cagctatttt tgacacagca tatacagatt
     4381 catctgaaac tgcggttaag attactataa aagcagatat gaatgatact aaatttggta
     4441 aagcaggtga gacaacttat tcaactgggc ttacatttga agatgggtct acagaaaaaa
     4501 ttgttaaatt aggggacagt gatattatag atataactaa agctcttaaa cttactgttg
     4561 ttcctggaag taaagcaact gttaagtttg ctgaaaaaac accaagtgcc agtgttcaac
     4621 cagtaataac aaagcttaga ataataaatg ctaaagaaga aacaatagat attgacgcta
     4681 gttctagtaa aacagcacaa gatttagcta aaaaatatgt atttaataaa actgatttaa
     4741 atactcttta taaagtatta aatggagatg aagcagatac taatggatta atagaagaag
     4801 ttagtggaaa atatcaagta gttctttatc cagaaggaaa aagagttaca actaagagtg
     4861 ctgcaaaggc ttcaattgct gatgaaaatt caccagttaa attaactctt aagtcagata
     4921 agaagaaaga cttaaaagat tatgtggatg atttaagaac atataataat ggatattcaa
     4981 atgctataga agtagcagga gaagatagaa tagaaactgc aatagcatta agtcaaaaat
     5041 attataactc tgatgatgaa aatgctatat ttagagattc agttgataat gtagtattgg
     5101 ttggaggaaa tgcaatagtt gatggacttg tagcttctcc tttagcttct gaaaagaaag
     5161 ctcctttatt attaacttca aaagataaat tagattcaag cgtaaaagct gaaataaaga
     5221 gagttatgaa tataaagagt acaacaggta taaatacttc aaagaaagtt tatttagctg
     5281 gtggagttaa ttctatatct aaagaagtag aaaatgaatt aaaagatatg ggacttaaag
     5341 ttacaagatt agcaggagat gatagatatg aaacttctct aaaaatagct gatgaagtag
     5401 gtcttgataa tgataaagca tttgtagttg gaggaacagg attagcagat gccatgagta
     5461 tagctccagt tgcatctcaa ttaagaaatg ctaatggtaa aatggattta gctgatggtg
     5521 atgctacacc aatagtagtt gtagatggaa aagctaaaac tataaatgat gatgtaaaag
     5581 atttcttaga tgattcacaa gttgatataa taggtggaga aaacagtgta tctaaagatg
     5641 ttgaaaatgc aatagatgat gctacaggta aatctccaga tagatatagt ggagatgata
     5701 gacaagcaac taatgcaaaa gttataaaag aatcttctta ttatcaagat aacttaaata
     5761 atgataaaaa agtagttaat ttctttgtag ctaaagatgg ttctactaaa gaagatcaat
     5821 tagttgatgc tttagcagca gctccagttg cagcaaactt tggtgtaact cttaattctg
     5881 atggtaagcc agtagataaa gatggtaaag tattaactgg ttctgataat gataaaaata
     5941 aattagtatc tccagcacct atagtattag ctactgattc tttatcttca gatcaaagtg
     6001 tatctataag taaagttctt gataaagata atggagaaaa cttagttcaa gttggtaaag
     6061 gtatagctac ttcagttata aacaaattaa aagatttatt aagtatgtaa tataaatata
     6121 ataatataaa aaggcttctt atatgagaag tctttttata ttatgttttc gaatacaaaa
     6181 aaagagacta aatttagtct cttttttatg tgagaaatac ttaaatatat aaagatgtaa
     6241 tttatttaag tatgattatt atactataca ataaaataat tgtctactat tctataattt
     6301 taaggtataa taataattgt atgataaaaa aggaggtcaa aaaatatgtc agttttagat
     6361 gcaatcttag ataaagcgga tgaacaagaa attaaaaaat taaatttaat agtagataaa
     6421 atagaatctt tagaaaaaga tatggaaatc aaagagaatg aacaattaaa agaaatgaca
     6481 aatactttta gacttagatt agacaaaggt gaaagtttgg atgatatact tccagaagca
     6541 tttgctgttg ttagagaggt ctcaaaaaga gttttaggaa tgcgtcaata taaagttcag
     6601 ttaattggag gtatagtaat tcatcaagga aagatagctg agatgaagac tggtgagggg
     6661 aaaactttag ttgaggtagc tccagtatat ctaaatgctt taacaggaaa aggtgtacat
     6721 gtaattacag taaatgatta tttggcagaa cgtgataaag aacttatgcg tccagtatat
     6781 gaatttttag gattgactgt aggtgtaatt ttatctaacc aagacccaag tataagaaaa
     6841 gaacaatata aatgtgatat aacttatgga acaaatagtg aatttggatt tgactattta
     6901 agagataata tggtgccaga tttgtctcat aaagtacaaa gggaactaaa ctttgccata
     6961 gtagatgaga tagactcaat attaatagat gaagctagaa ctccacttat tattgcagga
     7021 gatggtgatg aagatttaaa actttatgag ttagcaaata actttgtaaa aactgttaaa
     7081 gaagaagact ttgagatgga tagaaaagat aaaactatag cattaacagc aagtggtata
     7141 agcaaagctg agtcattttt tggtataaca aaccttactg atataaagaa tatagaatta
     7201 tatcatcata taaatcaagc tttaagaggt cataagctta tggaaaaaga tgttgactat
     7261 gttatctcaa atggagaagt aatgatagtt gacgaattta caggaagagt aatggatggt
     7321 agaagatata cagatggact tcaccaagct atagaagcca aagaaggtgt tgagataaag
     7381 aatgaatcta aaactatggc tactgtgact tatcaaaatt tcttcagact atatgaaaaa
     7441 ctttctggta tgactggtac tgcaaagaca gaagaagggg aatttgagtc aatctataaa
     7501 ttaaatgttg tccaaatacc aactaataga ccagttatta gagctgattt gcatgataag
     7561 gtatttaaaa cagaagaaga aaagtatagt gctgttgtag aagaaataat aaggatacat
     7621 aagactagac agccaatact cgtgggaaca gtttctgttg aaaaatctga gaagttatct
     7681 aaaatgctta agaagcaagg tattaagcat caagtcttaa atgctaaaca acatgataaa
     7741 gaggcagagg taatttctaa agctggtaaa ttagatgcta taacgattgc tacaaatatg
     7801 gcaggtagag gtacggatat ttctctaggt gcaggagata aagaagaaga agaaaaagtt
     7861 aaaaatttag gtggtcttta tgttatagga acagaaagac acgaatcaag aagaattgac
     7921 aatcagctta gagggcgttc tggtcgtcaa ggagacccag gtacatcaag attctttgta
     7981 agtcttgaag atgatgtaat aaagctttat ggtggaaaaa ctatagagaa acttatgaag
     8041 agaacaagtt caaaggaaaa tacggctatt gaaagtaaag cacttacaag ggctatagaa
     8101 agagcacaaa aaggtgtaga aggtaaaaat tttgaaataa gaaaaaatgt tcttaaatat
     8161 gatgatacta ttaatgagca aagaaaggtt atatataatg aaagaaataa agtgttaaat
     8221 gatgaagata taaaagaaga tattcaaaaa atggttaaag acatcataca agaagcagga
     8281 gaaacttatt taattggaag aaaaagagat tattatggat attttaaaca tttatatagt
     8341 acatttatgc ctgcagatac attactaata cccggtgtag ataaaaaaag tgtccaagag
     8401 ataattgata gcacatatga aatttcaaaa agggtttatg acttgaaaaa gatgatgatt
     8461 ggtattgata aagttgcgga gttagaaaaa acagtacttt taaaagtggt tgatcaatac
     8521 tggatagatc atatagatgc tatggaacag ttaagacagt atataggtct taaatcttat
     8581 gctcaaaaag atccgtttaa agaatatgct ttagaaggat atgatatgtt tgaagcttta
     8641 aataaaaata taagggaagc aacagtacaa tacttatata aatttaacta aaaaagttca
     8701 tgtaattttt attaaatgaa aaataaaata ttttgattct ttatattgta attacaatta
     8761 aataattaag gaatacaaat gaaaaagaag ctgaaaatca gcttcttttt ctgtgtgaga
     8821 aatacggaaa aaagagatgc ttgaaatttc tttcttccat ttaatattat actataaaga
     8881 agatgaaaag tctattagtt ataaactgga ttattaaaaa aaattacaat tttgttactt
     8941 ttttgtatac ataaatatta aaaaaataaa atataagaaa aagtttatat cttttggtta
     9001 attattacaa taagtctcat t
//
LOCUS       DAKBNN010000154         8985 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000154 DAKBNN010000000
VERSION     DAKBNN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.155"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..208)
                     /locus_tag="OUB02_002908"
     CDS             complement(<1..208)
                     /locus_tag="OUB02_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886646.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKC"
     gene            complement(709..1689)
                     /locus_tag="OUB02_002909"
     CDS             complement(709..1689)
                     /locus_tag="OUB02_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886647.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1853..2725)
                     /locus_tag="OUB02_002910"
     CDS             complement(1853..2725)
                     /locus_tag="OUB02_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8886648.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(2889..3440)
                     /locus_tag="OUB02_002911"
     CDS             complement(2889..3440)
                     /locus_tag="OUB02_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8886649.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3460..4362)
                     /locus_tag="OUB02_002912"
     CDS             complement(3460..4362)
                     /locus_tag="OUB02_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCT8886650.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4377..6749)
                     /locus_tag="OUB02_002913"
     CDS             complement(4377..6749)
                     /locus_tag="OUB02_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HCT8886651.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(6970..7659)
                     /locus_tag="OUB02_002914"
     CDS             complement(6970..7659)
                     /locus_tag="OUB02_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886652.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7902..>8985)
                     /locus_tag="OUB02_002915"
     CDS             complement(7902..>8985)
                     /locus_tag="OUB02_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8886653.1"
                     /translation="SVYYKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYS
                     NLIIILIYLIFSFFRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLH
                     SEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQE
                     SHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIII
                     NEKSEICLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYI
                     RIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGEL
                     LFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         2916 a         1578 c          845 g         3646 t
ORIGIN      
        1 tacattttcc acaacaatac tcatatgtct ccacattatt tggaatattt acaaatacaa
       61 attctttaca gttctcacaa tactttaata tagatatagt tccacaagtg taacattcaa
      121 atattacaca atcctcattt tcctctacta tttcaaactt tttactatgt tcaccacact
      181 ctgggcactt gtgattattt acattcataa atactcctcc ttattagtat agttacatgt
      241 caaaaactta attaatgtat tctatattac ttattatata ttctataata aatcatattc
      301 taatataata aaacaaatta aataaaaaaa aaccataaat tttaaattta tggattaatt
      361 cttgtaatga taactctatt tgaggatgtg agggagggtt atcattacaa tagggtatta
      421 tattatggtt tttaagctag tctttctaca cctcatatga tacatatgct atatgaattt
      481 tatatgattt ttacataaat attttatgaa atttaaattt tttagtcatt ttgaaatttg
      541 taaaaagttc tctaaaaatc cacagcatca taaacagcca aaatttaagt aaaacatcat
      601 aaaaaaattt gtccaataat tttaacaagt aatctcacct cattataatt attgacacaa
      661 acttaaataa acaatctata aatcttctat aaatcttaaa ctttccccct attgatacac
      721 aatttctagt ttgtcaattt tattttgcat ttctaagaaa atttcttcaa cagaattgtt
      781 aaatatattg cctaaataga ttcttttatc tttttgaaat gaataagatt ttacactacc
      841 atcagaagaa atatataaat gactccactc tgctccatat tttgaaaacc tctcctttgt
      901 atctttaaac ctcatatctt ctatttttat tgctttttct ctgtctcctc ttactacagt
      961 gtgacccctt tttattaaac tggttatttt tatatcttct tttaatttaa tacgtgtatc
     1021 atcatttttt gaatatactt tccatatcac tttatgacga taattatcta attcctttat
     1081 taagcttcta tcaatagtaa aactatcttt agttaccaca attaaatcaa aatggtctaa
     1141 catatattga aatatttctc tcatactttt attttttaaa atatcctcac caataacctc
     1201 tataaatcta cagcctttct catacatgca gttcaataaa cttgtgattt cagaaaaata
     1261 atcatttgta ttattatctc catctatttc tatagtaaag tacaaaggag taatgtatga
     1321 tttatttcct gttatctcaa atacacttga tagtgtctgt atatcatctt caaacgttaa
     1381 gtatacattc tcataaaaca taaaaaattc cataactttt tctttcatat tatggatatt
     1441 tctatatcta ttggataata tatttattat atcgtcagta gtatttttcc catcacattc
     1501 agccagtata acaatttgct tccaacttaa gctatccaca tttccgatac tttttttata
     1561 tccatcttct tcatcataaa tagggccaat tataatgttt tcttttggcc acagatgaac
     1621 cctagttcca tcttttaatt tcggatattt attttttaca gactcataaa atctacttgt
     1681 atcacccatg attctttact accccccata gtttaaatat tctatgctaa aaatagcact
     1741 ttatttttat taattcaaca attattcaaa attggtcatt atatatgttt aaatggattt
     1801 aacatcaacc ttatttatat gttattgatg tttatatatg ttatataaaa tcttatttct
     1861 catacttcaa tgtaatctta actatctctg gtttacaatt aagcctaatt aaaacttttg
     1921 agcctcctaa gcctttattt aaaatcatag tgctattatc tttttcatat tttccccagt
     1981 catacttagg aaagaactct ctatttggag ataatacacc tccaacaaaa ggtattctta
     2041 ttatacctcc atgaacatgt cctgctaaaa ccaaatctgc tccccatttt gcatattttt
     2101 caaagaaaaa tggtgtatgt gctaaaagaa tgttatattc attttcatta actttaccta
     2161 gattgtcttc tacaaaattg tcacttagtt ctaatttttt gttattagca aataaatatg
     2221 gatagcattc caatggtata attaaaccat ataaattaag atggctatta cctctatata
     2281 ttcttacaga ttcattttcc atatttatta ttttctttga atatagttcc ttaaaataat
     2341 ccttatatag atgtttatac ttttttatta aagatttttg ttcatgattt ccaattatat
     2401 gatatacttc atatttttca accaaattat ctattaaatc taatgcaact tgaaaatttt
     2461 tattttctcc atctactaaa tctcctgtta taaatataac atctgggttt aatgattcaa
     2521 ttttatctat caatacctta ttatttttat caaacacctt attatgtaaa tcactcactt
     2581 gaactatttt aaagttatca aattcctttg gaattttttt gttgaaaata gtatattctg
     2641 tcgtttctat atgctgagtt tcatacttat acagaatata caataatatt aatactaata
     2701 ctgtcattga aacctttata atcatattct cttcctcttt attctatttt gttaatatta
     2761 ttataatgct tttatgtttt ttttaaaaca ataacatcat cacatacata taaatctttt
     2821 cgacattata aaaataagtt atccttttct aaaattttca tttattttag aaaaggataa
     2881 ctataaaatt atctataaac ctctatattg tgaaatatag tttttccttt atttaaattt
     2941 ttatatatat gctctaaagt tccatcaaac ctaatagaat gtccagactc taaaatataa
     3001 gttttcccat ccacattaag gtcaagttta ccttctatta cgagtacata ctcttctaaa
     3061 acatgactat gtgtagatga aacatgagta cagttttcct caagctcaat tataaaaagt
     3121 tcaaaccttc tttgagggct aaaagggtat attggatata acttcatgtt ccctttttgc
     3181 tcaacaatag gctctacttc ttctttcttt ataacctcat aggtatttgt attttcattt
     3241 aatagtgtag taaaatttat tttaaatcca gatgaaattt tccataaagt agatactgtc
     3301 ggacttgatt cacctctctc aatctgtcca agcattgctt tactgacacc tgtcatcttt
     3361 gaagctttgt ctaaacttat gcccatttct tgtctcaata accttaaatt ttctcccaca
     3421 ttaggaaatt tattatccat gttaaaatcc tcctgatagc tatttgtatt ctatgatttt
     3481 tactcctgta agttctacta tctcttttaa ttttaacata tgattgttac aaatttcatc
     3541 aaattcatat atataatctt caagccctaa tgccttatat ttatcgattc caaatttatg
     3601 atatggaagt aactccatct tgcctcctgg aacattttgt ttaacaaata atgcagtatt
     3661 tcttatattt tcttcactat catttactcc ataaataatt ggcactctta taaccataga
     3721 tttattcaac tttgaaagcc tacatatatt atctaatata attttattac ttactcctgt
     3781 atattcttta tgaatattat catccataga ttttatatct acaaatatgt ggtctatttt
     3841 ctcaaataca tcatttacct tattccaatc aaaatatcca caagtttcaa ttgcaatatt
     3901 tatgccttta tcataaaaaa tatcaactaa ttctcttaaa aaatcaatct gaagtgtagg
     3961 ttcacctcca gaaaaagtta tccctccatt agattcaaag aaaaatataa aatctttttc
     4021 aacttcctct actatttctt caacactcat ttcttcggtc attatacaaa tggcatcttt
     4081 aagacatacc ttgatacact ctccacataa atcacattta ttatttattt gcgttctatc
     4141 aaatagtgaa cttatatttt gaggacaaac atctatacat ctattacagg atacacattt
     4201 atctttcata acacctaatt ttactatgtt actccaggaa tctggattag aacaccactt
     4261 acattttaat ttacatcctt ctaaaaatat agtagtcctt attccatctc catcatttac
     4321 tgaaaaatgt tgtactttta caactctacc ttttttactc ataatctacc tcataattaa
     4381 aaattgctat gtgaatttct agctattata gcatcttgca tttctcttga taaattaaca
     4441 aattgagtac tatatccagc aaccctcact aataaatctt tatattcttc tggattttct
     4501 tgcgctttta atagagtatc atttgatatc gtattaaatt gtacatggaa tcctcctaaa
     4561 gcaaaatatg atttaatcat agatgttaga tttctaagtc ctctttcagt ttccaccaaa
     4621 tcttgattca gtcttaaatt tagaagtgtt ccaccagtat gaacatcatg atttattttt
     4681 gatgcagatt tcattgctgc tagtggactt gttgtatcag taccaacaaa tggagaaact
     4741 ccctctgtca aaggttttgt cgcttttctt ccacaaggag ttgcacctac tatttttcca
     4801 gttggtacat agtttgatat acccataaat gcactattaa atttagagcc aaaaatatcc
     4861 ttgtattttc tagtttcagt ataataaaaa tcagaaactt caattgctaa tgaatctaca
     4921 taatcattat catttccata cttaggaaca tccaacgcaa gttttcttaa ttcttcataa
     4981 ccttcccaat cattatttag tgcttttgtt aattcttcta acgtagtcac cttatcctca
     5041 aatactaatt tttttatagc tgccaatgaa tttgaaacta cacctaaccc tataccagtt
     5101 aatacaggac ctatattata ttttgctcct cctttactta aatcaactcc cttatccaaa
     5161 catccatcta cacaagtaga taaaaatggt cttggtacca tttctttatg aatttgttga
     5221 gcaacagtag ttcctataac agaacttttt acaagatatg ctaattgctt taaaaactca
     5281 tccttaagtt cttcaaaaga agtaaattct gtgatatttt ttgtatctaa ccccattttt
     5341 tctccagtta atctactctt accttcattc atagcatatt ctaaagctgc tgcaaaattg
     5401 atatttacgg ctgaagtcca ctgtcctgtt tttctaaaat gaggaactac acaaccacag
     5461 ttactccaat ctctagcatc ttctgcatca tatccatctt gtaataacat ttgtgcccct
     5521 gcactatcgc tgtgtatagc agggaaacca gttccttgtt ttaccaattt agcaactgcc
     5581 attacgaaat tatctggtgc tccttgactc actctaacac ttaatcctgg ttggtgagtt
     5641 tttacacttt ctgtagcctt taaacacata tatgagatat cattcgttcc atcagtacca
     5701 tttctctttt taccaccaac agtaagattt tggaattgat tatatcctgc aaagtaattt
     5761 gctgtatttg ctgatatagt ccatacccat tctgataact taatccaaag tgcttctatc
     5821 aattcttgag cttctgattc agttatttta ccttctctag catcattttc ataatatgga
     5881 tacatatatt ggtcaaatct acctaaattt aatgccaatg gattttctga tagaatacca
     5941 cctaattgaa caaaccatac aaattgtatt gcttcataaa aattagttgg agggtttgct
     6001 ggtacattca tacatatctc tgaaattttt ataagttcta actttctctt ttcatctgtt
     6061 tcttgcttac tcatctcttt tgcaagattt gaatatcttt gtgccaatgt cataataccc
     6121 tgtgcaacta tagaaacaga tttgtaaaaa tctatctttt caatattttc tgaaactgat
     6181 atatctaatt ctttaatttt tttatctgca tcttccttaa tccctttata tccttttaca
     6241 aacaatatat cttgataatc tggtgttact tcaccaactg cctgtctcca cttagaatca
     6301 ttatctatta ttcctgtatc tactaatatc ttagctgtat cttctgggat tcttgctaag
     6361 aaaacttctt ccaaagattt tcccttccaa tatggaaata tttcttttct tataaattct
     6421 atttgttctt ttgatattac atatgggtct tgagttcttt tatcaaaagt atccatttct
     6481 ttgtctaccc attgccaaga aaactctggt gtcaatatcc ctgttcttct aaattttcct
     6541 gctgtaccta ctattaattc attttcaaag atttttatag gaagttcttt acatgtctcc
     6601 aaaaaagctt tagctcttct tatacagata gcttctccct cagttttttt atgtgattct
     6661 gtaaaaattc tagccctttc acaacatatc ataggtttag aattaatata ttcttgtcta
     6721 aatgcttcta ttctattagt ttgactcatt taaaatcccc ctttggataa tatatactac
     6781 atttttgtat gttataacat actttaatta tattatactg tcctttagca taaaatggaa
     6841 gcaaaataac ttattttttt tcatctacct tgaacaccta tatattaata ctttttttgt
     6901 taaatttata aaatctaaaa aaattttcta tgatatattt tctatattgt tatagttatg
     6961 tctaatatac taatgatttg aaatatcaaa tttgtatact agacttagtt ttaaaacctt
     7021 agttgattgt ttaactttgt taacttcaac atgtatatcc ttcatataag gaagctctac
     7081 aacacctact cccttattaa agtcttcttc aaccatagaa aaaatttctt tctcagttaa
     7141 gttcgtatta gtcaatgatt taaataaatt gtatataaat ttaatatatt tatcactctc
     7201 agataactta tcatcttctg gatactcttt agcacagaga atatctattg ttacttttcc
     7261 ttctttatcg tcttcagaca tagaatagaa taaacttcga atattttttt caggtggttt
     7321 ttcagcacca tttatagata ctacaaagct accatctgat tttgagttag taatactata
     7381 accactgtct attatagctt tcgaaaatct ttgagataaa tcttttttta cttcttctct
     7441 acctttcgca tcattaatct tttgtgagta attattaaat agctcgatag attcatcttt
     7501 tttatcttca acttgttctt tcaatgaatc tttactaatt tgttctgagc tacttaagtt
     7561 cttatcagat acaggagaca tacaccctac catcaatgct aagctaagat ttaaagccac
     7621 aattattgcc actctattta tattcctttt tactttcata cacaatcctc ccatcacact
     7681 aaccgctcta aagtattatt cattgcacta gaatatattt atattttctt tatattaaag
     7741 atttattatt caatacatat caccttccta tccaattctt ataacttact attagtaaaa
     7801 tattataagc tccttaatac agaaataagg atgttatctt ttgttattaa aagataacat
     7861 ccttattttt ttgtatcaat acatatgtcc caactggtaa cttaatcgcc ccaactgtca
     7921 gtatcttggt ccaatggtat atatatattt agtataaatt tatcgatact attttcaaat
     7981 aataattctc cattatattt ttgtaaagaa gatttaatac tctgaatacc tattccatgt
     8041 acgaacttat ccattttgtt tgttaacaat atatttttat taaaagtaag ctgatttagt
     8101 ttactatttt cacatttaag tacaaaaaat gaccttgtta tagttccttt aattctaata
     8161 tatttattaa cattttcatc cactatttta tcacatgctt ctattgcatt atccaaaata
     8221 ttagaaaata tgcttgacac atctataggt tttacaaaat ttacttttga aaaatttata
     8281 gagcaagtaa atttaattcc tttttttaaa catatttcac tcttttcgtt gattataatg
     8341 tctaatatca tatttcctgt attatagata tatttaaatg tttttatttc atcttttaaa
     8401 ttgttaacat attcattaat ttctttgcta ttgtttctaa ggttgtctat acaataaatg
     8461 tgattattta tatcgtgata tagtttctta actcgcatat gagattcttg aatacttaaa
     8521 taatgcgcat attgcatatc taacttattt tttattaatt cagcttgaga ttttaatcta
     8581 ttctctttta ttatttcttt aaacaatagt ataagaaata tactactagc tcctaccaat
     8641 tttggattaa caaaattatc aaaatagtat ccttctgaat gtaatgaccc aaaccgttct
     8701 aattttttta atacataatt tacaagcata ttcattgtat taactaataa ggcaaataat
     8761 aaatatacat aatttctctt atcttggcta attaatttta tagactttct aaaaaagcta
     8821 aatataagat atataagaat aattattaaa ttactatata tctgaatttt tatagattct
     8881 aagtgaggta ctaataaaga ttctttagat gaaataacat tacctgtaat tgcaaaaaat
     8941 agtattctat atataacagc atccaaacat ttataatata cacta
//
LOCUS       DAKBNN010000155         8933 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000155 DAKBNN010000000
VERSION     DAKBNN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.156"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            281..1642
                     /locus_tag="OUB02_002916"
     CDS             281..1642
                     /locus_tag="OUB02_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HCT8886654.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            1715..3109
                     /locus_tag="OUB02_002917"
     CDS             1715..3109
                     /locus_tag="OUB02_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886655.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            3171..3641
                     /locus_tag="OUB02_002918"
     CDS             3171..3641
                     /locus_tag="OUB02_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HCT8886656.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            3644..5860
                     /locus_tag="OUB02_002919"
     CDS             3644..5860
                     /locus_tag="OUB02_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCT8886657.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVTVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFYEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            5943..6614
                     /locus_tag="OUB02_002920"
     CDS             5943..6614
                     /locus_tag="OUB02_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8886658.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            6977..8191
                     /gene="dpaL"
                     /locus_tag="OUB02_002921"
     CDS             6977..8191
                     /gene="dpaL"
                     /locus_tag="OUB02_002921"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCT8886659.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            8318..>8933
                     /locus_tag="OUB02_002922"
     CDS             8318..>8933
                     /locus_tag="OUB02_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCT8886660.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMA"
BASE COUNT         3384 a         1039 c         1833 g         2677 t
ORIGIN      
        1 agaattgttt aaattattta gtatgagatt gttagattat ttttgatagc tattcatttt
       61 aataaaaaat ttaacaaata aatatatacc aggcaattaa tgttcattta agttgaaaat
      121 cggggaggga atatttagaa atttgttaat tttttataaa aatagctcag tttagctgtg
      181 tttatcggtt gagcaataaa ttcatggtca taaaaatatt tatagaaaat aattataaaa
      241 atatttagtc aagatattta tgtatgggag gatttatttt atgaaaacac gtaatacttc
      301 aaaatatgat gtcgatggga taccaccact aaaggaatca atacctttgg ccttacaaca
      361 tctgttagca atgatagtag gaaatatggt acctgcgata ttaatagcaa acgtggtagg
      421 acttaaccag gggcaagcaa caatgttgat tcagggttct atgttagcag caggtttagc
      481 aacgttttta cagttatatc caataccttt atttaaagga tttaagttag gttcaagatt
      541 acctgtaatg atgggaatga gttatgtatt tttaggagct tgtctttcag tagcagctaa
      601 agatggttta gcagctctat ttggagcaca gattgctgct ggggtaatcg tattctttgt
      661 aggatttgga gttaagaaaa ttagacatat attcactcca atagtttctg gaactataat
      721 agcatgtatg ggacttggac tattcgcaac agctataaag aatttagctg gaggagaagg
      781 cacacaaaca tttggaagcc caataaactt cacagtagga gttatagttg cttttgtaat
      841 aataatgata aataaatacg gtaaaggttt agtaaaaaat tcttcaatat taataggaat
      901 attagtaggt tatgctatat ctttagtttt aggattggtt gatttctcag ccgttcaagg
      961 agcagctata gtatctttac caacaccagc agcttttgga ttagaattta gaccagagtt
     1021 aatagtaatg tttactatta tttacataat aggtatagct gatatgatgg gtgcctgtac
     1081 aatagctaca gtaggagcaa tggatagaga ggtaactgac gaagaattag catcagttgt
     1141 tttaggaaat tctataactt ctataatttc atcactattt gcagctcttc caactggagt
     1201 atttagccaa aatacagtaa ttgtatctat gaacaaagtt acaagtagat ttgtaatagc
     1261 actaggggca cttgtattat tactagcagg gatatctcca gcattgggtg caataatgac
     1321 aactatacca tcttgtgttg ttggtggagc aactttagta gttttctcat caatagcaat
     1381 gtctggtttc tctattatga gtatggatgg attcactgaa gaaaacaatc ttatagcagg
     1441 tgtatctata gcaacaagta tgggtcttac aactgctcca caagttttag accaattccc
     1501 agaaacaatt ggaactgtcc ttggagggtc aagtatagtt tctggagcaa taatagctat
     1561 attacttcaa acattattta aattaaagtc acgtaaaagt gctgaaaacg ttacttcaaa
     1621 tcttgaggaa aatattggat aaatgtatta gatataatat gagaaaacca tatggtaaaa
     1681 tgttgtaatg aaagtattca aagggggatt taagatgatt gatatactaa taaaaaatgc
     1741 aataattgtt acagttaata aagacagaga tgttatattt gatggagcaa tagctattca
     1801 aggtgacaga atcttggaca taggaaatac agttgatatt gagcctaagt atcttgatgc
     1861 taaaaaagtt atagatgcta atggaaaagt aatattccct ggctttataa atactcataa
     1921 tcacctgttc caagtgttat taaaaggttt aggtgatgat atggcacttc atgagtggtt
     1981 aaatacaatg atgtttccat cagcaaaatt cttaacagaa caagatactt atgatgctgc
     2041 aatgcttgga tgtatggaag gtttaaaaag tggtataact acaatggttg attatatgca
     2101 tactcacaat agaccaggac ttacagatgg aatagtaaag gcatataaag atttaggcat
     2161 aagaggggtt gtaggtagag gttgtataga tttaggtatt cacaaagagc ttatagaaga
     2221 tgtggaaacg gttgaaaaag atttgagaag ggtattcgaa aaatatcata attcagaaaa
     2281 tggaagaata aaaatctgtg tagctccttc ttcaatgtgg gctatatctg aagaaatggg
     2341 taagatgctt tggaatatag ttaaagaata tgattcatat tttacagttc atatatctga
     2401 aacagaattt gcaagaacag caacaaagga tatacatgga gaaatagata taaaattact
     2461 tgaaaaatgg ggtatagtag gtccagaagt tgtagcagta cattgtgtat gtataactga
     2521 tgaagacatg gaaatgctta aaaagtatga cataaaagtt tctcataatg tagcaagtaa
     2581 tatgtatctt gcttctggtg ttgcacctgt gccagaaatg ttaaaaaaag gtataaatgt
     2641 gagtttaggt cttgacggtg cagcaagtaa taacgcacaa gatatggtag agcttatgaa
     2701 actaacagct ttacaacaca aagtaaataa ttgtgaccct ctggcaattt ctgcagagaa
     2761 agttcttgaa atggctacaa ttgagggagc aagaactctt agaatggaag atgaaatagg
     2821 ttctttggag attgggaaaa aagctgacct agtaatattt aactcaatgc tttctccaaa
     2881 atcaatacct cttcacaatc ctgtatcaac tttggtatac tcagctagta tgcacaatgt
     2941 agagagtgtt ttagttgatg gaaatgtaat acttgaaaat ggaaaagtaa ctacaattga
     3001 aagtgaagaa caagtgttgc ttaatgcaca acatgcagca gaagaattat gtaagagagc
     3061 taatgtaact aatagaatgg aaggacataa gtggaatagc ttatactaat attaaaataa
     3121 gattgtaata atattctatt acaacattag tttatctgta ggaggagaaa atggaaaaaa
     3181 taaaattaaa tattaatgat tacatgtatg aaatagaagt tgaaaagaat gaaactcttt
     3241 tgcatgtact tcgtgaaaaa ttaaatctaa ctggagcgaa acaaggttgt aatagtggca
     3301 gttgtggagc atgtaaagtt ttgatagatg gagttgacat gaaatcttgt aaacttcttg
     3361 caaaaaactg cgaaggtaaa gttatataca ctatagaaag ttttgctaat ggagaaaatc
     3421 ttcatccaat acaagaagct tttatagaag ttggagctgt acaatgtgga tattgtactc
     3481 ctggtatgat tataacaaca caagctcttt taaatagaaa tgcatctcct aatgaagatg
     3541 aaattagaga agcttttaaa gaaaatctct gtagatgcac aggatatgta aagattatag
     3601 aagctgtaaa attatctgca gctagattag ggggtagata attatggaaa aggtaatagg
     3661 aaaatcatac cctctaaaag atgcagcttt aaaagttact ggtcaaatga aatacgtggc
     3721 tgatatgaaa ccacaaaatg tactttacgc taagatgctt ttaagccctg tagcacatgc
     3781 aaaaataaaa agtatagata caagtgaagc tgagaaagta gtaggtgtta gagctatagc
     3841 tacttatctc aatacatcac aaagaaaata caatggttca ttaagatttt atgagcacaa
     3901 aatacctgaa aatgaagtta tattttcaga tacagttaga tttgttggtg accgtgttgc
     3961 agcagtagct gcagagactc cagaaatagc agagaaagct gtaaaactta taaaagttga
     4021 atatgaagaa ttgccagctg tatttactat acaagatgca ataaaagaag atgctatctg
     4081 catacatgga gattcaaata tagtttctca aaacaacata aatgggggag atgtagaaaa
     4141 aggcttcaag gaatgtgact atatatttga agatgaatac tctactccag caatacatca
     4201 tagtgcaata gagactcata gtgtaattgc acactatgat tataatgata aattaactgt
     4261 acatacacct tgccaaaata catttggatt tagaataata ctatctcaaa tttttgggat
     4321 gtcattaaat aaagtaagag ttataagacc tgctattgga ggctcatttg gtggaaaatt
     4381 agaacttaca attgaaccag tagcagccgc attatcaaaa atgacaagaa gaccagttaa
     4441 aatggttctt acaagaaagg aatctatgat atcaacaaga actcgtcatg ggtcttttgt
     4501 aaaattaaaa actggtgtaa ataaagatgg tgaagtaata gcacaagaca taaaaatata
     4561 tacaaataca ggagcatatg catcaagtgc tttaaatgtt ataggagcac ttagtcataa
     4621 agtatttaaa gtttataaaa taccaaatat aaagtttact gggatgcctg tatatacaaa
     4681 tacaccaata gcaggagcta tgagaggcta tgggtcacct caaatattta tggctcaaca
     4741 agctcaattt gcgaaaatag caaaggaaat aggtatggat ttagtcgatt ttcaaaataa
     4801 aaatgcagta gagccagatg atgtcgatat aatcttccac ggttctcttg gaaatcctag
     4861 agtattggat tgtatagagc aaggtaagaa gatgtttaag tgggatgaaa agaaaaaact
     4921 tccaaaagaa gaaggaaaat atttaagagg tataggtatg gcaataggtg cacatggtaa
     4981 tggagttttt ggtgcacata gagatgttgt tacattgact cttaaattaa atgaagatgg
     5041 tacgttgaca ctactaacag gagctcatga tatgggtaat ggtgttgtaa ctatgcaaac
     5101 aatgatggta gctgaagttt taggaataac acctgataaa gttgatacat ttgaaactga
     5161 tacggatgca tgtacattca accttggtga ctatgcaagt agaggggtat ttgtagaagg
     5221 tggaggagct aaaaagacag cagagaaatt gaaaaatctt atattagaag aagcagaaaa
     5281 attacttgaa acatcaaaag aagatttata tctagacaat ggatgtgtaa ttagtaaact
     5341 tgatgaaaat gtaaaagcat cactttctga tgtcacagta tatagtcaaa gtaaatcatt
     5401 aagagagtta actgttgttg aagattattc ttcaccagct ggacgtactt cttatggtgt
     5461 acattttgct gaagttttag tagataaaga aactaaggat ataaaagtag ttgattttgt
     5521 agctgtacat gatgttggta gagttataaa tcctctaaat ttagaaggac aattagaagg
     5581 tgggattcat atgggtctag gatacgcatt gagtgaagaa ttggcatttt atgaacaagg
     5641 tagacctaaa gctactgatt ttaaaacata taaattattt agaactgtag atatgccaaa
     5701 atgtcaagtt gcatttgtag atggttatga aaaagctggg ccttttggtg gtaagagtat
     5761 tggagaatgt gccgttgtac cagttgcgcc agctgttgca aatgcagtat ataatgcaac
     5821 tgatgtagag atacattcat taccaataaa attaaaatag tagaaattaa agcagaaata
     5881 acaatttatt gtatttgtaa atagataata aaaatagatt tatttggtga ggagatatta
     5941 ttatggcaaa gatattaggt atttgtggaa gtccacgtaa aggagcaaca gaatatgcag
     6001 tgttagaagc tttgaaagaa gcagaaacta tacctggtat agaaactgag tattggtcag
     6061 ttagagggaa aaaaataagt ccttgtgtac attgtgatgc atgtataaga aaagaaacta
     6121 tgtgtattat aaaagatgat atccaagaat tagagcaaaa aatattggag gcagatggaa
     6181 ttatagtagg ttctccagtt tatgatatga atataactgc tcaacttaca gcagtattta
     6241 atagacttcg tccaatgtat ttagttcatc caggaaaact tcaaaataag gttggtgcag
     6301 ctataactac tggtggaact agatatggtg gccaagaatt gacaaaactt cctataataa
     6361 atttcttcct aatgcatgaa atgttagtgt ctggaggttt aggtggatgc tatataggtg
     6421 gtacaatatg gagtaaagat ggaaaagcaa aaggtgccca agaggatgag acaggtatgg
     6481 acacagtaaa aagacttgga aaaggtgcag cagaggcagt tatggtttct aagtttggtc
     6541 ttgaaaaatg gaatgtcgta aaagaagaat tagatgttaa aaaagatgaa aaatcacctc
     6601 ttagagacca ctaaagattt attatattat tgaatatata caaatagtga tgtaatagat
     6661 tattgcatca ctattttatg ttttaattta gattttataa attttatatt aaaaaatata
     6721 gaaacaaagt gttttgagat ttatttttaa attttaaaaa tgagaattaa aaagtgagaa
     6781 gatagaaaag gttttttagt tattaaattc tacatacatt gtatgaacta aagatagaaa
     6841 agaaaaaata ataaaagcat tgcaatttct atactaaaga gtcatggaaa aatatctagt
     6901 atatatttag tattggcatg ttaattgctt atatattaaa gtataaaaat ttaacaataa
     6961 tttggaggta ttttagatgt taaaagaaat taaatggaaa gtaaataatt tgccaaaggg
     7021 cgataaggaa aattgtatca aatttttaaa tgaagaagaa ataactaagg ttagaaattt
     7081 tcacaaaagc tttcctcaat ataaagaaac tccattagca aacctagaag gtttagcaaa
     7141 aaaattaggt gttgcaggag tatatgtaaa agatgaatct tatagatttg gattaaatgc
     7201 atttaaagta ttaggtggtt cttattctat gggtagatac ttagctcaaa gattagatac
     7261 agatatatct gagttagggt atgacaaatt aacttcaaaa gaaataaaag aaaaattagg
     7321 agaaataact ttctttacag ctacagatgg aaatcacggt agaggagttg cttggacagc
     7381 taataaatta ggtcaaaaat ctgttgtatt aatgccaaaa ggttcttctg aatttagatt
     7441 aaataaaatt aaaggagaag gtgcagatgc tagtataaca gacttaaact atgatgatgc
     7501 agtaagatta gctaatgatt atgcagaagc tgatgaccat ggagtaatgg ttcaagatac
     7561 agcatgggac ggatatgaag aaataccagc gtggataatg caaggatatg gaactatggc
     7621 tcaagaagct atagagcaat taaaagaata tggagtagat agaccaacac acgtatttgt
     7681 tcaagcagga gttggttcac ttgctggtgc agttcaaggg tatgttgctt caatatatga
     7741 tgaatgccca ataacagtag tagtagaggc tgacgaagct gattgttact ataaatcagc
     7801 agaagctgga gatggaaaac caagatttgt tggaggagat atgccaacta taatggcagg
     7861 gcttgcttgt ggagaaccaa atactatagg atttgaagta ttaaaaaatc atgcagcagc
     7921 atttgtttca gcaccagact gggtttctgc taaaggtatg agaacattag gtaatccttt
     7981 aaatggtgat gaaaaggtaa tttctggaga atctggagca gttacaactg gtcttttagt
     8041 agcagctatg gaaagagaag atttagcaga tttaagaaaa gatttaaaat tagatgaaaa
     8101 ttcaagaata cttttaataa gcacagaagg agatactgac cctgacaaat atagaagtat
     8161 agtttgggat ggagaatatc caagtatata aggaattact aaaatgcaat tattagatat
     8221 tggtttacat atttagaaga tatataagtt ctaaaattta gaaaatatat aagtatataa
     8281 aatacataca ttataaaaaa tctaggggga taatgaaatg ttaaacgaac aaagaaaaca
     8341 agaagtaact gaaatatgcc aaaaattaat tcaaaatcct agttcttctg gaaatgaaga
     8401 aggtgttgta aaagcaatag aagagtcttt tgaaaaatta ggttttgata gttggtcaag
     8461 agatagatat ggaaatatag taggatgtat aaaaggtaac aagccaggta agaaaatctt
     8521 atttgatgga catatagata cagttccagt tccagatgca agtaaatgga gtgttcctcc
     8581 ttttgaagga aaaatagttg atggaaagat atatggtaga ggaacttctg acatgaaagg
     8641 tcaatatact gcaatgatgt ctgctgtagc gtattttgct aaagacacta atagagattt
     8701 tgctggagaa ctatatgtag caggtgttgt tcatgaagaa atatttgaag gtgtttcagc
     8761 tagagagata agtaaggctg ttcaacctga ctatgttgta ataggtgagt cttcagagtt
     8821 aaacttaaag attggtcaga gaggtagagg agaaatagta gtagaaactt ttggtaaacc
     8881 agctcactct gctaatccag aaaaaggtgt aaatgcagtt tataaaatgg cta
//
LOCUS       DAKBNN010000156         8925 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000156 DAKBNN010000000
VERSION     DAKBNN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.157"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(302..808)
                     /locus_tag="OUB02_002923"
     CDS             complement(302..808)
                     /locus_tag="OUB02_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HCT8886661.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(1179..2207)
                     /locus_tag="OUB02_002924"
     CDS             complement(1179..2207)
                     /locus_tag="OUB02_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HCT8886662.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(2255..3115)
                     /locus_tag="OUB02_002925"
     CDS             complement(2255..3115)
                     /locus_tag="OUB02_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCT8886663.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            3384..3593
                     /locus_tag="OUB02_002926"
     CDS             3384..3593
                     /locus_tag="OUB02_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HCT8886664.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            3593..3859
                     /locus_tag="OUB02_002927"
     CDS             3593..3859
                     /locus_tag="OUB02_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HCT8886665.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            3888..4460
                     /locus_tag="OUB02_002928"
     CDS             3888..4460
                     /locus_tag="OUB02_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCT8886666.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(4694..5884)
                     /locus_tag="OUB02_002929"
     CDS             complement(4694..5884)
                     /locus_tag="OUB02_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCT8886667.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(6144..6575)
                     /gene="dut"
                     /locus_tag="OUB02_002930"
     CDS             complement(6144..6575)
                     /gene="dut"
                     /locus_tag="OUB02_002930"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCT8886668.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            6688..8916
                     /locus_tag="OUB02_002931"
     CDS             6688..8916
                     /locus_tag="OUB02_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HCT8886669.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
BASE COUNT         3128 a         1403 c         1084 g         3310 t
ORIGIN      
        1 tatttacatt aaaataaatc aaatacaaat gccataaatt aagtgtttct agttagcata
       61 aaaatactta taaagtatac tcaagtattt caaataaata cttttattca aataaatctc
      121 tacttatata agtgcttata taaataaaaa gatgaactat agtaaatata gttcatcttt
      181 tttataatag tatttattta aaattaatat ttatttagtt actagcatct acttagatat
      241 cagataagaa agcacagtca tttacttata ataagttctt ttcatatatt ttttattata
      301 attattcttt tacaagaact tttataattt ctgaaatgta catagtatgg taatctttct
      361 ctggatacca ttttttatca ttttctttag catcaaagtt ttctggtggc atattaccat
      421 gatatagctt tttacatact ataatcatat tagcttcttc aaaagctacc gtatcatcca
      481 caaaatatgg tgtaagtcct gctttttcta ttttatttat atctctacca gaaatagtgc
      541 cacaaatatt taaagcttct ctatacgttt catcaaagaa ggcaactgta aatgtatcat
      601 tactatctac aaattctttt gtatatctct gtggtcttat gtaagttgta acaacatttt
      661 taccccaata gactccaaca ccaccccagc tagcagtcat tgtgttaaac ttttctgaat
      721 caccagctgt tataagtaac cattctttgc caatcttagt aaatggatta aactgtaatt
      781 cctcaatttt tacttctcta aaacccatga cttcatccta cccttcattt tttaattata
      841 taaattaatt gttaaaaatt tgaatatatg tactcttata atactgactt ttgattgtcc
      901 tacataattc atgtaaatta taatacaaca aatcaattac tttcgcaata agtagttttc
      961 atattttata agtgtaaaat atagataaaa tatattgatt ataaagtata taatatattt
     1021 ttaaacctaa agcaatatac aataaaaaaa tgtagaaagt aagtatctct ctacattttt
     1081 tattgtatat tatttttata ttaatttaat tttatcttga ttcagtttta taaaaaattg
     1141 ttataattgt caagttctac tctattattt ataatttctt atatcttatt aacaacattt
     1201 aagttattac atttatttat atatattttt tcattatcta agtcttcatc aaacattttt
     1261 attatatttt ttatctcata cttgtactca tcttcattca ttctataaag cattaaatct
     1321 tctagtacat ctttcatatc gaagtacaca tcttctaata aataaaaata gccttcctct
     1381 atatctttca atttatctat agtatccatt tttttactta tatctttcat aatttctaaa
     1441 ttttgtatat ttacatctat gattgttaat aagtacattc tcacagtttt tattatttca
     1501 acagaaaaat ctacaatatt ataatttcct tcttttttat ataatgactc cttaaatttg
     1561 ttaaaacaat tatctaaatt agaatttata tcgttaaatg gtggtcttat tatgtcacgt
     1621 attattaaaa gattttgtat atctacatta tttgtgattg cctcatccaa aataaataaa
     1681 ttatatgcat catttaatgg gtcatgggca tctccactaa actctaagtt aacaaattca
     1741 agtaaatttt tcaatgatat atagttcata catctcatac cacaatttat atatttttct
     1801 tttatatcta caaataaatc tctaatgtta tttacaaacc ttggatggtt atttttttta
     1861 gcacttctca tatctgtgtt attgaaacat gtcaaatcca aattaccaaa agtatatatt
     1921 cctttaatat cagaaaatat gccaagccat tttttaaact ttctcatgac ctcttcataa
     1981 cttggtgcac tcttcaattc ttcagaagat atatgtgtta attcttgtat atgaggatac
     2041 aactcctcat tacttactgg tcttattaaa gatttaaatt tatcaatatt ttttgttttt
     2101 tcatcataca taactgctcc gattgcaatt atatcagaat taaatactgc tctttttgat
     2161 ttagaatttg gcatattcat ttcaaaatct atatatactc ttttcaatat aaacaccctt
     2221 tccaattgtt tatagtgatt ctttttaact atttttattc aaaattttct aagaaagaat
     2281 gtttctctcc atctttttgc caaccaagta ctgaatcaag atttacattt tctgccgcct
     2341 taaatataga tttttctaca tttttaaaat ttgattctaa actctttatt aagtctttta
     2401 tctcatatct tttattacct gtttttttag cagcaaccat acatgcacaa tttaatggcc
     2461 atataccact attttgaata aatcttataa tatgttcttc tcttatataa taaagtggtc
     2521 ttataatttt tataccttca aaattagtag aattaagttt aggaagcatt gttttaaaat
     2581 ttccagcaca taataagtta agcattgttg tctctattac atcgtcataa tgatgaccta
     2641 atgcaagttt gttacaccct aattcttctg cctttgaata taatgctcct ctcctcattc
     2701 ttgcacacat atagcatgga taatcttttg ctatttcatc tgctatctca aatatccttg
     2761 aatcaaataa atgtattggt atatttagat actcacaatt atctataagt aattgtctta
     2821 tatttgcatg atatccaggg tccatagcaa taaattcaac atcaaaatta acttgtccat
     2881 gtttttttaa ctcttgaaac attttagcca taagaatact gtcttttcca ccagaaatcg
     2941 ctacagctat cttatcacct tcttcaacca atttataatc tcttattgcc ttcataaact
     3001 tagaccataa atttttccta tattttttta ttatactttt ttctatatct cttactggaa
     3061 gtctttcctc aaaaggaact ataatttcac agccttttcc tgatagttca ctcatctaat
     3121 cacctcgtat atttatgtta ataatatttt acaaaaaagt ctaccttcat ccacaaaata
     3181 ctataatttt tactttaatt ttactaaata atttatagtt tacaataaca cataaactta
     3241 taataacata aataacctct ttcttttatt atctttttta tttttctcat ttaatatttt
     3301 attattagta acgattttta aatgattgaa tattatacac tagatagagt tacctatcta
     3361 tttacaacaa ggaggttttc atcttggaaa ataagactag agatatcaaa aaaaattgtg
     3421 gtccatcttt ggctagacca tacattaata atcaaatttt cacagaaatg tactgccttt
     3481 ctgatgcatt aaaatttgga actatatttc cagaactaaa cttactagag tctgaaatgt
     3541 acaacaaaga attatatgct aagccaaaaa aatatagagg aggtagaaga taatgaaaga
     3601 ctcatctaga gctgaactta tgagaaaagt tcaggaaaca tcttttgcat gtgttgatat
     3661 gaacttatat ttagacaatc accctgatga taaaaatgct atcaacactt ataattcatt
     3721 atgtaatcaa tttgcgcaag ctagatatgc ttacgaaaat aaatacggtc ctttaactaa
     3781 ctttgggtat gctccaagta gatgcccttg gcaatgggct gaccaacctt ggccttggga
     3841 cagagaattt aattattaga catcaagtat tggaggattg aaaattcatg tggatatatc
     3901 agaaaactat acaacaccca gttaatataa aaacttgtga ccctagaatg gctaaatttc
     3961 ttataactca atttggtggg ccaaatggag aacttgctgc atctttaaga tatttaagcc
     4021 aaagatatac aatgcctact ggtaatatgc gtgcactttt aacagatatt ggtacagaag
     4081 aactagctca cgttgagctt atatgtacta tggtttatca gttaacttct gatgcaagcc
     4141 cagaagagtt aaaagctgca ggtcttggtt caaactatgc tcaaaatgga tatggaattt
     4201 atccaacaga ttcaaatggt gttccatttg atgtaagacc tatagcagtt atgtcaaatc
     4261 ccgtaaccga tttacatgag gatatggcag ctgaacaaaa agcacttgca acttattatc
     4321 aacttataaa cctaacagat gacgttgatg ttatagatgt attaaaattc ttaggtcaaa
     4381 gagaaataat tcactatcaa agatttggtg aagctttaat ggatgcttac gagttagaag
     4441 aatctcaaaa aatgttctaa aatttttatt tgtataacta ttattatttg ataatttata
     4501 atttactgtt gtgttaaaat ttataaaaaa tataaatata atttattttc tataaacaga
     4561 gaaaataaat aaaaagcagg tatcaataaa atagtaacat gtattatact atacattact
     4621 atcaatattg tacctgcttt catattataa gcaataaaga aaaatctatt tattaggttt
     4681 tataataaaa caattataaa actcttcttg catttacata tctacttgaa tagtagctac
     4741 tatttatatt agatattgtt actttttttg atgaacctga agatgcatgt atcatatctc
     4801 catttcctat gtatattcca acatgactta cagaaccatt atttgaacct tgtgtatcaa
     4861 agaatactaa atctccactt ctaaggtctc ctctatttac atatgttcca tacttacttt
     4921 ggtctctaga tactcttggt atactaaccc ctgcagattt tttcataacg tactgtgtga
     4981 atccagaaca gtcaaaacta tttggacctt ctgctcccca tacatatggt tttccaagta
     5041 gtgtctttgc aaaacttatt acactatcag ctccttgact tggagaatta gtcgaataag
     5101 atggaattga agtatcaaca gaaccttttt cagcaaggta ttgacttgac acatatccag
     5161 tttgattgcc ataacttact tttgtccatc ctccactttc agaaatactt ccaacctcag
     5221 taccataact taatgtagct attttagaat gacttgtacc tggtcctgtt ctaacgttca
     5281 atgactttgc tgttacaacc tttgtaccct ttacaaggtc atttgaactg ttttcagcat
     5341 tttcatttgt agagtcattt acactagttc ctaaatattt acttgaaaca taacctactc
     5401 taccattaga acttattttt gaccatccat ttgactcaga tatgtatccg acttcagtcc
     5461 catatcctag tgttgatatt ttagaactac ttgtacttgg tcctgttcta aaatttaatc
     5521 cttttgctgt tacaactttt gtcgacttaa ctgaagtatc actactttca ttgctacttc
     5581 caaggctatt tattgctaca taatttccat gcacataacc tgtgtttcca ttatatttaa
     5641 cttttaccca agaaccttct tttcctacgt actcaacttt atcacctttg taaagtttcc
     5701 ccattgattc atggtttgta cctggtcctt ttctaaaatt taccgcattc cctgttattg
     5761 ttctgctttc tatactcaca ctctctactt cagactcatt agcatatgct aaattcccca
     5821 tagtaggcat tattatcgca gtcgccatta tactggctgc tattctcttt tgattaaaat
     5881 tcataacttt ccactcatct cctcttttct actatcttta tttcacttca aaatataaaa
     5941 gaaataattg acactattaa aatatcaatt atttctttta tattaatatt attacaaatt
     6001 tgtaactatt tgtctatagt aacatttgct taccataaat taccatgtat aaattttaat
     6061 ctttaatagc ctgtaattta gtaatatagc catcttttac cttttttaaa aatggctata
     6121 ttttttaact tttttgtaac tttttaaaat ccagttgagc caaacccacc ttttcctctt
     6181 tcggagtcac ttaattcttt tacttcttct atgttaatat catagatagg ttttactatc
     6241 atttgagcaa ttttcatatg cttttcaacc ttaaaatcat ttttaccatg atttattaat
     6301 ataattttaa gctctcctct gtatccttcg tcaatagttc ctggtgtatt taatacagta
     6361 acagaatgct ttaaggcaag cccacttcta ggtcttacct gcgcttctgt catagtagga
     6421 agctctatac atatcccagt tttgataagt ttagtctctc caggaggtat tacaacctct
     6481 tctatagaat acaaatccat accagcatca cctttgtgag caaaattagg tattatagca
     6541 tcatcattta atttttttac ttttaaattg tacatcttat atctcctttc aactttctat
     6601 aaatattata acttaaattt cgacctcttg aagtttttta tttttttgtg gtaatataat
     6661 tattagtgtt ttaaaggagg attttttatg aaacttgtac ttgccgagaa accatctgtg
     6721 gcaaaaacta tagcatcatt tttaggtgca aagaccagac aagatggtta ctttgaagga
     6781 aatgattata taattactta tgcagttgga cacttagtat cattatatga tatgaaggat
     6841 tatgataaag ataaatactc tggctcttgg aaaatgaaca atttcccatt tgttcctgag
     6901 gataagttta aatttaagat tgatagttct aaaactaaac aattcaacac tgtaaaaaag
     6961 cttttgaata gaaaagatgt agaatatgta ataaatgcca ctgacaatga ccgagaagga
     7021 gaacttatag catttttaat atttttactg gcaaaaaata aaaaaccagt aaaaagaatc
     7081 ttggtaaatg aatggacacc agaagatatt acaagaggta taaaaaacct aaaagatgaa
     7141 gatgaaatga gaaatcttca agctgctgga tatactaggc ttattactga ctggttgatt
     7201 ggaataaatt tcacttctgt tgctacattg aaatatggaa atgggaaact attaaacata
     7261 ggtagggtaa ttcttccaac agttaaatta gtctatgata gagatatgga aatactaaac
     7321 tttgttccta aaacttatta tgaaatagaa gggcatttta aagctgaagc tggtgaatat
     7381 aaaggtaaat atgttaaggg taaagaaagt aaatttgata cacttgaaga tgcaaataaa
     7441 attatagctt caattacatc tgaaactggt aaaatactag ataaaaaagt tactatgtca
     7501 aaggaatatg caccaaaact ttttagtttg acatcacttc aaggatatat aacttctaag
     7561 tattccaatt ttacatctga taaggtttta aatgtctgtc aatctctcta tgagggtaat
     7621 ggtaagggtg gatacataac ttaccctaga actgattcca ttttcttaga agaaagtttg
     7681 gcttcaaaag cttctcaaac tttagataaa ttaaaggttg gtcttgaata tgaaaataaa
     7741 attaaatttt ctaaaactaa aagagttttt gattcttcta aagtagactc ccatagtgct
     7801 ataattccaa cttatataat tccaaaaagt ttatctaagg atgaacaatt agtttatgat
     7861 gccataaaag atagatttgt agcaaatttt atgcctcctg ctgaatatga gaatacagaa
     7921 attaagactg aagtagataa ttgtactttc ttaacaaaag gtaaggtgct taaatcaaaa
     7981 ggataccttg aggtatataa taaggaagaa aaaaacgatt tacttccact tgtaaataaa
     8041 gatgatgttg tagatgtgtt agaaataaaa ccattaacta agcaaactac tccacctaaa
     8101 ccatatactg aggacaccct tttaaaggct atgaaaaatt gtggtaaaaa tgtgcctgaa
     8161 gaagatacaa cagtgctatc tggatattcg attggaacat cagccacacg tgctgatgtg
     8221 ttaaagaaga taagtcaagt aggctatgta aagaaaaaag ggaaatctta ttctataaca
     8281 gaccttggga aaaatttagt tgaaattttt cctgttaaag acttatttga tgtagattat
     8341 actggaaaac ttgaaaaaag tttaagcgat attcaaaaag gaaagtatac aagaaaagaa
     8401 tatcttacta atatcattag ttttatatat caaaatgtca atattataaa acaagatgct
     8461 tcaaaaaata taaacacaga agcatatact ttcgattcaa aaactaaaaa atttactaaa
     8521 acaaaagaaa aaaaacagaa aaaagatgta actaatactg aaaaagaagt tgctatatca
     8581 aaggattcta ataacaaaaa agaagttaaa aatggtgatg aaaatcaatc tcttggcaaa
     8641 tgccccattt gtcaaggtga tgtactagaa tttgataaag gctttgcttg taaaaatcat
     8701 aaagaatgta aatttgttat ttggaaaaat gataagtatt taagtcttta taaaaagaaa
     8761 gttaacaaaa caatggtcaa aaatatttta aaaaaaggtg aaactaaagt aaaatcttta
     8821 actgctaaaa acggtaataa atttgatgct acattaaaat atgataaaaa tccagatact
     8881 ggctatttca attggaaaat agagtttgac aactaaatat tcata
//
LOCUS       DAKBNN010000157         8705 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000157 DAKBNN010000000
VERSION     DAKBNN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.158"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            305..457
                     /locus_tag="OUB02_002932"
     CDS             305..457
                     /locus_tag="OUB02_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886670.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            651..1535
                     /locus_tag="OUB02_002933"
     CDS             651..1535
                     /locus_tag="OUB02_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HCT8886671.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAIT"
     repeat_region   1841..2326
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1841..1868
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            2482..2670
                     /locus_tag="OUB02_002934"
     CDS             2482..2670
                     /locus_tag="OUB02_002934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886672.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            2790..3604
                     /locus_tag="OUB02_002935"
                     /pseudo
     CDS             2790..3604
                     /locus_tag="OUB02_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            3656..3817
                     /locus_tag="OUB02_002936"
     CDS             3656..3817
                     /locus_tag="OUB02_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886673.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(4145..4609)
                     /locus_tag="OUB02_002937"
     CDS             complement(4145..4609)
                     /locus_tag="OUB02_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886674.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVFEDMKNKILRERKYKKRR"
     gene            complement(4745..5062)
                     /locus_tag="OUB02_002938"
     CDS             complement(4745..5062)
                     /locus_tag="OUB02_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886675.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(5345..5641)
                     /locus_tag="OUB02_002939"
     CDS             complement(5345..5641)
                     /locus_tag="OUB02_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886676.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(5660..6073)
                     /locus_tag="OUB02_002940"
     CDS             complement(5660..6073)
                     /locus_tag="OUB02_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886677.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(6057..6290)
                     /locus_tag="OUB02_002941"
     CDS             complement(6057..6290)
                     /locus_tag="OUB02_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886678.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            6509..6883
                     /locus_tag="OUB02_002942"
     CDS             6509..6883
                     /locus_tag="OUB02_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8886679.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            7173..7691
                     /locus_tag="OUB02_002943"
                     /pseudo
     CDS             7173..7691
                     /locus_tag="OUB02_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8123..8377
                     /locus_tag="OUB02_002944"
     CDS             8123..8377
                     /locus_tag="OUB02_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886680.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            8413..>8705
                     /locus_tag="OUB02_002945"
     CDS             8413..>8705
                     /locus_tag="OUB02_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886681.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRV"
BASE COUNT         3527 a          920 c         1379 g         2879 t
ORIGIN      
        1 aaaaaaaata cttattgatt atattattta attattttat gtagatattc ttaggttggt
       61 atggaatttg tactaaccta ttaagtaatc aataaaacta aataaacata gaaagcactt
      121 acaaatatgt aggtgctttt tattgaaaaa atagaaaata aaataaaaag taagatatag
      181 gaaaaatatg taagaattat atgttataat aattgtagca agaagatgta atctacaatt
      241 tatagagtgg agttcatgta ttaaaaaatt atcctcccaa cgataagaag ggaggtgaat
      301 atacatggat aattttttat ttaatgtgtt agctagtgta acagctagtt acatagttta
      361 tttgattagc aagttaatca aaaaagtaaa aagccactct accgcaaaga gtgacttaaa
      421 gattgatata aattttaaat atcatcgtaa aaaataactt tcagttatag aacttcactc
      481 taccgcaaat agattgtagt tcttcttgtt tttattatac cacaaattag aaaaaatatt
      541 gcatataata ttttttatag tcaataaaaa tatgaaattt tttatacaca atgatgatga
      601 atctaactag atgaataaat ttaattgtaa ataagaaagt acttacttaa atggtaggtg
      661 cttttgtttt gccaaattat gctataatat tgagtgagat gagtaatttg tgggggaatt
      721 ttataatgtg gatattagta ccaataataa caatagtatt acttataata gctgtttctt
      781 ctatgcaata tattttagtt atgatagctt ttttgttgat tatatattct tttatagaaa
      841 agaaaatagt tatgggtctt gtgtcagttc tttttttcac ttattcaatt tatctttgtg
      901 caacttggga agataaatcc ttgatagcag ataataaagt tgagactgtt aaggcacaaa
      961 gagaaactgt agaaagagaa aaagaaatgg aaagaagaag aatacaagaa gaagtagaca
     1021 aagaaagata tattgaaaaa catggaatgg aaatatcaga aaatgattta aaagttaagc
     1081 tagaggcttt agtacctcaa gaatataagg ggaagaaata tgaattaaaa gtaggaaaat
     1141 ttaaaagata tagtatgtat tttgatttaa ctgtacaaaa tgaaaaattt agtaattctg
     1201 aagaatgtaa aaaatttgta aaagaaattg ctaatgatct taaaaaaatt aaaataagca
     1261 aggcgtactt taagtttcat tcaaaagatg acggcggtat atataattct gtttatattg
     1321 attattttag aaacatacaa aataatgtag ataatgttga aaatttagaa ttcaatgagt
     1381 ttgaacttaa aaccgaagaa gaagagaagc gagaacaaga gaaaatagaa caagaaaaaa
     1441 acagttataa taactacatt caaaacagag tggttgaccc actagataga ataaagaaac
     1501 taaaagagtt gctagattca ggagcaatta cataggaaga atataataaa aagaaaaaag
     1561 aattattaga atagataata tagtaagcac ttacaggtat gtaggtgctt ttaaatttac
     1621 aaagtattcc attttaattt tatagtttag attttatgat ataataaaaa tatagaagtt
     1681 ttgcagtgtg cgatatttgt tacaaagtag ggcttaatac ttgaaatcta agatgttgag
     1741 ggtgcgtgat aagtgttatc aattgcacta ttgccccctc actgcaattt taagagtatt
     1801 gtatatatgt aggtattgga aatgctaagt ttattttggg gttttagatt aactatatgg
     1861 aatgtaaaaa tttttgacgc tatgaagaaa tttactacaa aatttgtttt agattaacta
     1921 tatggaatgt aaaaactttt ttcatcctcc caaacctctt gttcgctgtc gttttagatt
     1981 aactatatgg aatgtaaaaa agaaaataag tttaaaaaaa ggtttcatta ttatatcgtt
     2041 ttagattaac tatatggaat gtaaaaccaa ttatgtacag tttagttact gtgttaagtc
     2101 ttgttttaga ttaactatat ggaatgtaaa atccttttat aaatatcttc tgacatgtgt
     2161 atatgcttgt tttagattaa ctatatggaa tgtaaatcgg ctacaacggt ataaattaaa
     2221 tttagcaggg tacattttag attaactata tggaatgtaa attgctgtga tttcaataat
     2281 aaaacaatta ataattgtgt tttagattaa ctatgtggat tcaaaattaa ataaacaaag
     2341 aaagcactta caaatatgta ggtgctttta ttctgctcaa aattggtcgg ttgggtaaaa
     2401 taattagaaa aagttagtaa aaacctattg actgtaactc gttacaatat tgttattaat
     2461 gtaacgagtt acagaaaaga ggtgaataaa atagcaacta aaagtagggc agagtatatg
     2521 aaaaatcgtc gaaaagataa aagaggtttt agtgtacttt tagacaaaga aaagttagat
     2581 aaatttgatg aagtgttaga ggaaaagaat ctaaccaaga aagaatggct agaagaaaaa
     2641 atcgacgagg aactggaaca aaaggaataa aaaataaggg tcactcccac cgaccaaagt
     2701 ttgagtaacc cctatgacgt atactatcgt atatcaatta tagtatatgt cattccttaa
     2761 aaaaatcaat tattaaggag tgtaatatta tgaaaaatga attaatgatg tttgaaggaa
     2821 aagagattga agtatttgaa tttgaaggaa gaattttatt caactcaaaa gatgtagtaa
     2881 actgtttaga cataaaaaat gttaatgaga atataagact tatgaatgaa aagcaagttg
     2941 taaagttaag aaattcggat atcagtaata ctgacatccg aaaacttaat aatgctggag
     3001 aaaattttct tactgaaagt ggagtgtata aattaatctt taaatcaaga aaagaagaag
     3061 ctgagagatt ccaaggctgg ataagtgatg aagttttacc aagcataaga caaactggtg
     3121 catacataaa taataatgca ataccgaaaa gttgagagaa aaagcaagtg agattgaaaa
     3181 attacagttg gtttataaca gtacatctat gttaaaagaa ttgctagatg atgcaggctt
     3241 tgacaataaa tcaaaactat taacagctaa aacattatat aagaaagcag gaattgattt
     3301 accaatagag ataaacgaag aagaacatta ttttgataca aaacaaatag catctaaact
     3361 gaaaatatat tctaagagta ataaaccagc tcagatggca gtttgtgaga ttattaaaaa
     3421 gattgattta gaagatggtg aagttaaagg tgtttgggaa attaatggtt cttggactgg
     3481 tactgtaaat aaatatacaa agagtgtaat agataaggtg agaacttgga tagaggaaaa
     3541 taatagacct accaagattg caggtgagaa gaagaattat tatgtatttt acaaaattga
     3601 gtaaatttat cagttgtatt aaataattta gtttagtttt ggggggatta atacaatgtg
     3661 tgagaattta cttgataata tgtatattga aaagagaaaa gaagaatata gaattaaact
     3721 tttgaaaata agagaaacag atatagatat atataataag ttagaaagta tagtatataa
     3781 actttctgag aaaaaattag agaaaaataa taaatagaca tataaagcac ttggatattc
     3841 tactgtttca agtgcttgtt tggtataaaa tggtataatg gagatagaga attatattaa
     3901 ccaaaaaatg gagaaaatag agaagatgat ttagttgacg ctttatcagt aggagtacct
     3961 gtaggtaaga cagaatctct agtacgatta gttggagatg gaataagtga tactcaaaaa
     4021 agtttaataa ataaaagaga atttagaaat ataactcaaa ttggtggaaa tggaaatgag
     4081 aaagcattta gtgaagtaga aaacttagtt aaatagatta gaaggtagca actagagttg
     4141 gttgttacct tcttttttta tactttctct ctctaagtat tttatttttc atatcttcga
     4201 atacatctaa tgcagtagta taaaattgta tattatttct ataaggtata gctattggaa
     4261 gaatgccctt gttaactaaa aaaataagaa gtacaataaa taaaagtgtc ataatcattc
     4321 taataaaata attagtgata tctgataaat caaatgttaa gttaattgtt gtagaaaata
     4381 caacagccat aatagatata tacaaagcaa aaatggggtt actatttttt tgtaactgtc
     4441 ctttaagcct agattgctct atttctatat cttgaatact ttttatggaa tccatttcac
     4501 tataatactt ataaactttt aaataatatt ttttatactt gtttaaagct tcttcattat
     4561 ctttaaggtc tttgtcactt aactctgatt taacttcccc aaaatccata aaatccctcc
     4621 tacaatgtta tataatatac tttaatttat agcatattta agaacttata tcagcaattt
     4681 gtcgaacgat tttagctaaa aatagagtat aatgataggc ttattattta taagatatta
     4741 ttttttatat agttatatat ttctttctaa ttagataata taatgctgtt gttataatgg
     4801 ctactaatat tattgctatt attgtaatat ctaatcttct tgtaaataag cctattgcta
     4861 tatactcaag acctgttaca tagcgataaa gtacttctag tttaacagtt tttttcttat
     4921 ctatatttcc atatattgat tctaatttat taaaatattt tttactacaa aaagcagttt
     4981 taggtttaat taaatatatt attgtagttc ctattataac tattattcca ataagtataa
     5041 gattgatatt tccaaaatcc atagcagata cctcctatta aatttgcttt tcttaatcaa
     5101 agtatatcag taacacaagt acaaagagta tctaaaatat tacaaatatt aacaggatag
     5161 ataaaactat agtaaaaatt gtaataataa agcgaacgaa aattatcata ctaaaacata
     5221 agacatgtta taattgtatt agatagaaga ttaacataca ccaaagcact ccttataata
     5281 aagagtgctt atttttttga aattcatcaa tatataaact atcaagaacc ttattcaaca
     5341 caccttaaaa tcgatttaaa tagctctttt tgataacata atttatatga tataataaaa
     5401 aaatagaagt gtaccaccaa tacacttcta tagctataac taagcattct cttgctgggg
     5461 gagtgctttt ttcatttctc caaacaacat tatttacttc atcagtaatt atcaaatcaa
     5521 tctcattatc tttcaaaatc tctgttgcag tacaaattag attatctaag tcaaaacttc
     5581 gagttatctc tagttgtttg ttatttactt ctctttttag tatataagtt ttaaaataca
     5641 tttatatcac cccttgtatt tactcttttt tctctaaaaa tatattgata agtaaatatg
     5701 caacaaataa aaaaatctca atgacacata gtgttatagc tataactaga agtgattgat
     5761 ttttccatgc actataagct atgatgaaaa gaaatgtaaa agtaaataat gtataattca
     5821 taattttaac taagatatga cttgttttca ttgttaagta caagtctctt tcatctgtac
     5881 tatcgtgtaa ttcttcaaca atgccttttc ttgaaaaagc attacaaaaa ttaaagattg
     5941 ctattacaat taaaagaaat gatgaaatta ggaatctttt atcttttaaa atgcaataaa
     6001 taactaaact agcacaacat aacacacaaa ctataatacc cactataaaa cttcgtttac
     6061 tctttacttt catgtttttt atcctccatt tctttatttt cttttaaaca acatagttct
     6121 tcaatcgttg taccaaacac ttgtgccata cgataggcaa gcataagaga agggctatat
     6181 tgctcttttt caatagaaat aattgttctg tttgatacat ggactaaatc agcgagttgt
     6241 tgctgtgtta gacccatatt tgttcgaaat tctcgtactt ttgttttcat tccataacct
     6301 ctttctatat gaagctaact tcatgtgaag ttaacttcat aatatcctaa aatgagtaaa
     6361 atgtcaatct cattagataa gtattataaa acctaaaaga ataatatggt aaaaattgga
     6421 tggaattatt taccttaatt ctcttgtttg taaaacttta ttgatataga atttaacata
     6481 tacataaatc ggcaaagggg cgcgaattat gaaaattagt aaattaccag aagcggaatt
     6541 aaaagttatg agatatattt gtgaagctaa caaggtatta acttctaggg aattagttgc
     6601 tgcaatggaa gaaaaatatg agtggaatga atcaactaca tttacagttt tgaaaagatt
     6661 agagcgaagg gagtttttaa gtacagagaa aataggcaaa cttacacatt ataatatact
     6721 agtaaaagag aaaaaatatc taaggtttga aacaaaggaa tttttgaaga acatacataa
     6781 aaattcaatt tcaagtttaa tgtcagcgtt acatggtgaa gacgacgaag tggatgaaga
     6841 taaattaagt gaactcgaag aatgttttaa gaatttgaaa taagaaaatg acaaattgat
     6901 acataataaa taaaatgaag gtatgaaatt ataacttttt ctaccttcat tttgtttatt
     6961 attctgaaca tgttaagaaa atgaaataag tttgtatttt atttatataa attttaatag
     7021 aatatgtata gtatatagta gatagaatgt ataaaaataa aaactgttca ataagaatta
     7081 atatacatta attcagctga atccaatcaa aatacttaaa taaaagtaat ctgaaaataa
     7141 cataattcaa atcaatttat tcaaataaac ctcaatttat ctcaaatctt aagaagtcat
     7201 taaacaaact tgctaaatta caaagagaat tatcaagaaa aacaatcggt agtttaaata
     7261 gaaataaagc aagattaaaa gttgcaagac ttcaagaaca tatagctaat caaagaaatg
     7321 attttctaca aaaactatct actaaattaa taaaagagaa cgatattatt tgcatagaag
     7381 atttacaagt aaagaatatg attaggaatc gtaaactatc tcgctcaatt tctgatgtgt
     7441 catggtcaga gtttattcgt caattagaat acaaggctaa ttggcatggt agacaaattg
     7501 taaaggtggg taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg
     7561 aggaagtgaa ggacttaaat ataagagaat ggatttatcc tagttgtaat gaaactcacg
     7621 atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta ataacaattc
     7681 aaaataaata acgtatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt
     7741 aagacatatg ttagtatgca aaattctgtg aagcaggaaa cctgtgactt tagtcatggg
     7801 aggttcaggc tagtattact cattattgta ttcaaaagct aaagtggaaa cctagtgaat
     7861 atatgaattt agaagttaat tagagagcat tagcagccga ctcaatactt attaaaattg
     7921 aagatgaaga agaaacaatg aaagaagctg aaagagagaa aaagagaggt agaagaaggt
     7981 agaatttaaa gttaaaatag ttacattgct aatatatgaa aatgttacaa atttggaaat
     8041 agatggatat atatgttgta tagtataatg tagataatat atcaccaagt aaaaaacaac
     8101 aaataaatct aaattatgag gggtggaaaa taaagatatt aaacttacaa tatgtatatt
     8161 tgcttttgta attggaataa tagtgttatt ttgctgtata ggtctaggtc aatttaatat
     8221 ggattgtatt ataaaacaaa atggtgggtc aatagctacc gaacaataca acatatattt
     8281 agaacaatct atttcacaat atagaaattt tggttctata ctggttttac taggaggaat
     8341 aggaatactg tttgataaaa gtgtcagaaa atactaaaaa agtatatttt tatttaggat
     8401 gtgatgaatg atatggcaag agatgtattt agtgtaaaga tgacaccact agaagcacag
     8461 aaaatggtag aaaaaaattt ggaagcagat ttagtttctg ttgatattta taacctagaa
     8521 aataataagt ctataatagt tacaatcttt gaaaaatatt atgcaagaag caatagtgat
     8581 gcaggtttgg tagtgatttg tgaaaatact acaggaaaaa cacttgttaa aattacctca
     8641 actggtagtg caattggact atttcagatt gattggggaa caggtaataa tttgattaaa
     8701 agagt
//
LOCUS       DAKBNN010000158         8702 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000158 DAKBNN010000000
VERSION     DAKBNN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.159"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(493..1245)
                     /locus_tag="OUB02_002946"
     CDS             complement(493..1245)
                     /locus_tag="OUB02_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCT8886682.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1304..2959)
                     /locus_tag="OUB02_002947"
     CDS             complement(1304..2959)
                     /locus_tag="OUB02_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8886683.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(4232..5311)
                     /locus_tag="OUB02_002948"
     CDS             complement(4232..5311)
                     /locus_tag="OUB02_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8886684.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(5395..6051)
                     /locus_tag="OUB02_002949"
     CDS             complement(5395..6051)
                     /locus_tag="OUB02_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8886685.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            6373..6579
                     /locus_tag="OUB02_002950"
     CDS             6373..6579
                     /locus_tag="OUB02_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886686.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6732..7160)
                     /locus_tag="OUB02_002951"
     CDS             complement(6732..7160)
                     /locus_tag="OUB02_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCT8886687.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7636..8574)
                     /locus_tag="OUB02_002952"
     CDS             complement(7636..8574)
                     /locus_tag="OUB02_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HCT8886688.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKKLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
BASE COUNT         2797 a         1604 c          943 g         3358 t
ORIGIN      
        1 atgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt cctgtataaa
       61 atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg ttttatattt
      121 tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt ctttatattc
      181 tttcatcgca tcattccaag cgtcttgtac tgttacacct cttttattag caattttttg
      241 tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc ctcctacaaa
      301 ttaccatata tttcatatat ataaatcaag gttatatttt tatgatatat aatccttcta
      361 ttgtaagatt acagtttata tgtgtattat atatgaacta tattagaaga taattagaat
      421 ttttataaag ctatatgatt agattaaata taaaagcttt acaattactt ataaatttaa
      481 aaagtctata agctaaacta atgtttgata taccaatttt acgaccataa ataatgccat
      541 tactaaaaat attggtttta tcatctttga acctttcttt atagcaactt tagcacctac
      601 ataagctccc attatcatca ctaatgcaaa cactatagca attttataat ctacttgacc
      661 atttatcatg aataataaca atgaagttat attacttgtt aagtttaata tttttgtatt
      721 tgcagaagca tgtaaaaaat catatccata tatttttata aatccaaagg tcaaaaaagt
      781 tcctgtacct ggaccaaaaa aaccatcata aaaacccatt ataagagcca taagcatccc
      841 tagtcttaaa tttttcttat taacagcttc aaaattatct tcttgaccta aatttttaga
      901 aataaaggtg taaatagcca ctattaaaat cataaccaca accaaaacat ttaatatctc
      961 ttcacttatt gataatacac atctcacacc tagaacacta ccaataatag taaatggtac
     1021 taattttttt aaaaggtcgt tattaatttt tccagacttt gcatatctat aggcactact
     1081 tatacatcct gctgatgctg caaatttatt tgtaccaatg gcaagatgta ctggcactcc
     1141 aattgccata agaacaggca tgcttataag tcctcctcct cctgctatag aatctacaaa
     1201 agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat tcctccatca
     1261 ttctctttca atttttctta caatatatat tatataatct tatttaatac ttattatcca
     1321 gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat atttgtgaaa
     1381 aataactact gttattaaaa cctactttct tacaaatatc actaatactc atgcttctat
     1441 ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca gttattgaaa
     1501 ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa aaaacatctg
     1561 atatactatt caaatttata ttttctttat aatatttttt tatatattcg tttatatttt
     1621 caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata cactctttgg
     1681 aaaattcaaa taatacattc cataaatctt catcactgtt tacaagtgat atttttttat
     1741 tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta agcactgttc
     1801 tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat cttaatttgt
     1861 ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct ttttcaatat
     1921 aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct atcggattaa
     1981 taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct tcaagtctta
     2041 tattttcttt tctactcttc aatatctcct catcttttat actaagttga ttttcaaaaa
     2101 aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt tcttttaata
     2161 catataacct acttttagga tttaatggaa attcattgta aattttgttt atgtatttta
     2221 tatcaatacc agaactactt gataattcag ataaattttt atctatatca atagacttat
     2281 atactataaa attaccacat tctatatatc caactatatt ttcttctaat actataggta
     2341 tctctaaact ggaaacacca tactcacaat ggtacacttt ttcaaagtta tcattattta
     2401 tgcttttaac aagtttttta gtacactgtt ttatattttt acaaatatcg caaaatttat
     2461 tttctttctt agtctcaaat atttctttat actttaagtc atatacagat attctaagct
     2521 tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta actaactcct
     2581 gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat ttatgaggaa
     2641 cataagtaat taatattact aaatcaattt taccagtgtt tataatttca tgttctgaat
     2701 atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca tcaattattt
     2761 gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga aaatgctttt
     2821 cttggcagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg aattgtgttt
     2881 ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact tcattatttg
     2941 actcaataaa atccaccata aactcacctc gtagtttttg tctaaatatt ttttcttccc
     3001 ttcttatatt aaaataatat catcatcatt aactttcatt actataaaat catcatactc
     3061 ttttaatact cctgtaattt tagttgcact tagtttacta caattcagtt ttttaatata
     3121 ttgataaatt ctataattac ttttttattt tgtaaaatca gtgttttccc aaaataatta
     3181 tatctataaa tgaaagtatt tttactgctt acatcaatat atgttatcat aattttcctt
     3241 tttattgcat atataaataa ctgtatataa cattgtccaa tactatatag aaatacatct
     3301 aagaaagaat attattaaat tgttccaatt agtattattt ttcttataaa aataaaaaaa
     3361 ctgttggatt cttttagaaa aattattttt tacatacctt ttttgcttag atagtctatc
     3421 ttctcagcta ttttgtatat acactcttct agttcatgat tttattttaa tatcttgctt
     3481 atattgctga aataattgaa ttcttttatc tatcatattg tctcttatat cataaacata
     3541 ttgtttctta tatcacaaaa atgaaagcat tcttatatct tcaatatata aacctgcttt
     3601 ttttaatctt gtaataaata taggatactc tatattctct tcctagaaat aactaatacc
     3661 acttattttt ctattatata gaaagtaata aatctttttc ttaataaaat tcaatagtaa
     3721 gtggaataaa attttatatg aataagaaca ttataaaaat actttataca atagtcaata
     3781 aaagatatta gcttcaaatt atgttaaagt tgtattattt aaagtgaaaa aacaaataaa
     3841 aaagaaataa tagtataagt agaaaaatat acttaaaaca aggcgaagcc tctcttgtat
     3901 ctaaatcaag tagagaatta aatctaaaaa agaaaataaa tataaaagat gactcctgta
     3961 cattacaaga atcatcttat ggtttcataa tcactccatg gtttttaact gtctgatata
     4021 aaggtctcag ttcactcata gcatgaccac cctttccgat tgctgacccc gtcacttatg
     4081 gacaaaatgt ccataagtga tagtcctctc ttaaaaccct ctaaatagat atatctcaag
     4141 cacctcattg aaaccagttg ctggaaacaa actgaagcgt tctggaatag taaaagaaat
     4201 tgccaccata tccacgttac tgtcctagtt gttactttgc taacttcgga gcaatttcct
     4261 ctaacaatac gcagtcaaat tctgcctttt tgctaagaaa tggcagatgg tctgcatctg
     4321 gtataaaaaa aatctgtttg tctggcgcca ctatttcatc aaaaaatttc ttcgctggaa
     4381 gataggggct attatagtca ttttctccca tgatgtagta tacagggacc tgatagtcaa
     4441 ctccataatt tcttatgtca gcttcttcca ctaaaaaccg caatacatca ccttgattgt
     4501 caatcgcatt tggattgaca atggccgtat tttttaactc tctcaatgaa taatacgggg
     4561 ttgccagtat actgattata ttgcccaaat ccatatttat agaaagccca tacttgcttt
     4621 ggtatgttct tactgtcata agagctgcca aatagcttgg accatatttt gctggcggat
     4681 atggtgcaat agcttccagc gccttgatat ctttttgctt ccctgcagct ttcaccattt
     4741 caagtgtctt ttcatagccc acgcgctcat tgtccaccat attaacggtc tgcgcaatcc
     4801 caatgtacgc gctgtaatcc tccggatagg tttgcacagc catagttccc agcactgttc
     4861 cccagctatg acctataagg ataattttct ccttgttgaa ctcttgcttc aaatatgctg
     4921 tcacctcatg gacgtcttgc agaacacgct ccgcgctcat ggtctgcaaa actgctgccg
     4981 gatcgttcgc aaagtatgtt ttgcctacgt ttcgctgatc ccagttcacc acagtaaaat
     5041 cattttccca ctcatactgg tacagatgta aaaatggcat cattgaatta ccaggacccc
     5101 catgaaccca aaggatgatg gggttgtctg cgttttgtcc ccggatatat aacgcctgct
     5161 gtatgccgcc gatctccacc atttccatgc ggtcgattcc aggagcttct attttatggc
     5221 ttgtacgtat aatcgtgggg gctatcagac gcaaaccggc aaacgccaga acaaaaacag
     5281 caactagtat gcaaagtgtg attaatatca ttttaagtac cttctttcat gaattttttt
     5341 agccatagat atctcctcta acattaaaaa aaatatgttg ctattttagt tttgttactc
     5401 tacctgtccc attggtttcc tttcataaaa aatattaatt gattctataa tctcatcttc
     5461 caggatatca actcctattt gtctcataag aaactgtaag aattgaaacc gtttacgaag
     5521 tgtaggaaag tccccctgaa atacatctgt atcacaccaa aagttaaaga gcagcaaaat
     5581 tacctctgcg cattcatctg ggaactctgt gatgatgctt ctatcctcta tgccttctcg
     5641 taaaacattt gctaatattg gtgctagttt tttcaaatta tcctgtgtaa atgccagtat
     5701 gatatgcgga ctttctatag cgcttgtaat catgttacta gattctttta tcacctcatc
     5761 cgttaaatgc cgcctaacta aacttctaag cttctctttt gctgtaagtc catgcatttc
     5821 accgagccat tgatttactg tctctattat ctgttcaaag cgcctttcca tgaccacatt
     5881 aaaaatatct tcttttgaac tgaagtgatg aaaaattccg ccctttgaca ttcccgaagc
     5941 atcaacaata ttttgcatac ttgttttgtc atatcctttt tcagcaaaca attttgcaga
     6001 aatggtaatg atattttcaa tagcttgctt tggattgttc tttctagtca ttatataact
     6061 ccttcttatt aaaaaccgac tgttggtttt tattatataa gctattccta cttctgtcaa
     6121 gcaacattat gtttatggca aaacaataag gtttggtggt tagacaggta ttaattagac
     6181 taatgcgact ttcccctaga tggaaccctt ggtaaatttg caatctttac cggctttgat
     6241 tatatctgta agcaaggcag gtatggccac acctgccctg tttgaccctc ggatattcat
     6301 tcctatccct cctctcctta gccattacac caaaacgccg ctcattgtct ccaatggcgg
     6361 cgttttaaag atatgatttc tcatgctgct gaaatatgca aaaaatataa gatgcccttt
     6421 attatttttc ctacaataga aaagaaaagt attgaagaat gggttaatat tggagcaaat
     6481 atgctttgtt ttgcacaaga tactaccttt atcacttatg ttatcaatca attattatct
     6541 ttttataatg agattgtacc aattcaaaaa caacattaaa ttataaatct aattttatat
     6601 taaaagagac taatatttta tctatctcta ttaaggatat tttcttatgt atcataatat
     6661 tagtctctct tattattata tactgctatc atcattagtt gctatttata ctatttttat
     6721 aatatacaaa tttattcagc taacgttttg cctaattcta tacactcatc ttttgcttct
     6781 tcatttgggt cttccattgc tgtaattcct tctccaccaa taattgtagc accagcattt
     6841 tgcatacgtt cttcccagtc tctcatccat tcacaatttc cccatccata tgaaccaaat
     6901 aatccaatct gtttacctga taccatagat tctaattctg aaacaaatgg ctccatatac
     6961 ccctcttcta gttgctctgc tcccatagat gggcaaccta atgcaaattt atcttcttct
     7021 tttaaatcac ttggtttcag taagctcaca tctaaaactt ctggtgtctt accttttaac
     7081 ttcacacctt cagccacaaa atttgccatt ttttcagtat ttcctgttcc actccaataa
     7141 acaatatata ttttactcat ttttacctcc tgtagtattt tttaattaat caatagttag
     7201 ttatagctaa ctaacatcta aaaaacaaaa gatttcttta tctgaaatct ctttattttt
     7261 ctaaatatag aatatcatag ttgattttga aattcaatac ttattgataa tgtttatcat
     7321 ttatatttta tattcatttt ataatatcaa aacactgtat cgtatttcta atctttcata
     7381 gaaaaatttc taacttatag tacttttgca tctcttataa atcttttata tgtataagta
     7441 catagaaaaa cacacctttt ccagatgtgc tatctataat atcgtatatt tatttttcaa
     7501 ttctaactag ctaatatgtt tataaatttt atcacttaat acctttatac attgatgtaa
     7561 aatcttacac ctaagtaatt tttattattt atctcgactc tatataatat agctatttta
     7621 tcatcaaatc aaaaattatc ttttgccatt attcttgtat ttataaagat ttaaatataa
     7681 gtatccattt ccttgttccg ttctaggtat tccaagcgct ctcgtacatt ttattaattg
     7741 tgcatatagt tcagcttcat ctaaatctct ttcaaactcc tctcttgccc attctttaat
     7801 tagtgctaat gagcctgata cgtatggtgc actcatactg gtaccactca taatagcaag
     7861 tttattatcc atataagtcg atataatatt tcttcccgga gctaccaaat ctattgtagt
     7921 atttgaatta ctaaactttt caaccaaata gttttcattt attgcaccta cttctattac
     7981 ctcagcataa ctggctggat aactatactc acttgtacta gaatcaccat ctccattatt
     8041 acctgctgca cacactacag aaatattgtt ttttactgct tgcatgacag cattttttaa
     8101 cttcttatca tctttgtttc ccccaagaga catagatata atatcaacct tgttatttac
     8161 agcaaagtta atagcattaa ttatactttg atatgttcca gtaccatctt tatttaatgc
     8221 ttttgctatt aataatttac aatctggagc tactcccata acttcattat tatagttaga
     8281 tgcagctata atacccgcca catgagttcc atgaccatta aaatcctcat atatattttt
     8341 atttccatta ctgtcatcac taaaatttgc accaccaatt atttttcctt ttaaaagagg
     8401 atgagatata tcacaacctg tatctattat accaactaca atatttttac cagtataacc
     8461 ttcatcccac attcccctag cattcatttg ttttattcca tatgggaatt tattttttgc
     8521 acctcttaga ttttcattta tttcatacgg aattagtttc attcttttat tcatcatata
     8581 tcctcctaaa aattttattg cttataaaaa tcatgaattt tattttataa taggtttata
     8641 aatcaaatta aaacctatat ctacttattt atatttatga tatatttttt atttttagga
     8701 gt
//
LOCUS       DAKBNN010000159         8667 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000159 DAKBNN010000000
VERSION     DAKBNN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.160"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(32..193)
                     /locus_tag="OUB02_002953"
     CDS             complement(32..193)
                     /locus_tag="OUB02_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886689.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(521..961)
                     /locus_tag="OUB02_002954"
     CDS             complement(521..961)
                     /locus_tag="OUB02_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8886690.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASDRFVDENGKPVEWELKVLSSE
                     EDESLRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1033..1503)
                     /locus_tag="OUB02_002955"
     CDS             complement(1033..1503)
                     /locus_tag="OUB02_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCT8886691.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1520..2830)
                     /locus_tag="OUB02_002956"
     CDS             complement(1520..2830)
                     /locus_tag="OUB02_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCT8886692.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENV
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(2831..3007)
                     /locus_tag="OUB02_002957"
     CDS             complement(2831..3007)
                     /locus_tag="OUB02_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886693.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3000..3437)
                     /locus_tag="OUB02_002958"
     CDS             complement(3000..3437)
                     /locus_tag="OUB02_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886694.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(3786..4592)
                     /locus_tag="OUB02_002959"
     CDS             complement(3786..4592)
                     /locus_tag="OUB02_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886695.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(4702..4992)
                     /locus_tag="OUB02_002960"
     CDS             complement(4702..4992)
                     /locus_tag="OUB02_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCT8886696.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(4992..5339)
                     /locus_tag="OUB02_002961"
     CDS             complement(4992..5339)
                     /locus_tag="OUB02_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886697.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(5333..5734)
                     /locus_tag="OUB02_002962"
     CDS             complement(5333..5734)
                     /locus_tag="OUB02_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886698.1"
                     /translation="MGSNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELEEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNNILTFRCLRW"
     gene            complement(5744..5977)
                     /locus_tag="OUB02_002963"
     CDS             complement(5744..5977)
                     /locus_tag="OUB02_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886699.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(5977..6876)
                     /locus_tag="OUB02_002964"
     CDS             complement(5977..6876)
                     /locus_tag="OUB02_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HCT8886700.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGV"
     gene            complement(6888..7451)
                     /locus_tag="OUB02_002965"
     CDS             complement(6888..7451)
                     /locus_tag="OUB02_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HCT8886701.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITKEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(7517..7717)
                     /locus_tag="OUB02_002966"
     CDS             complement(7517..7717)
                     /locus_tag="OUB02_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886702.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            complement(7732..>8667)
                     /locus_tag="OUB02_002967"
     CDS             complement(7732..>8667)
                     /locus_tag="OUB02_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCT8886703.1"
                     /translation="VNVGWSLMSLDTNRINKIISKPWATDGLNFSERIWGKYRPTLVN
                     ELHTKLTQSIIRGENPKNLVNDFDKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLD
                     VEKYEIIATLDLRTSNICRELDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGY
                     RAARGEDGKTYYVPSSMKYNEWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIH
                     DGKQGKHLKEHNNYIEGRSYLTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVD
                     KNIGVNVNNITGEKTLTNKFKIHYSKTGTHIVPAL"
BASE COUNT         2490 a         1708 c          876 g         3593 t
ORIGIN      
        1 gcggtagagt gaagttcata attaaatgaa tctatttgaa cttatggaac ttgattttaa
       61 aatcaaattc ccagccactt ttactcttgc cacgagttga gtggcttttt acttttagaa
      121 atactttaca aattaagcaa aatattaaac tagcaataac gccagctatt acattaagta
      181 aaaagttatt catacttccc acctcctttc attaggaagt aggttttatc ccagtatgaa
      241 ctccactcta taaattgtag attacatctt cttgctaaaa atattataac atattttacc
      301 tattctatat ttattttttt attttgctat cttcttcgtc ccctctttct ctctctttca
      361 gcttctttca ttgcttcctc ttcatcttct atctttataa gtattgaggc ggctgctaac
      421 gctctctcat taacttctaa attcatatat tcacttggct tccactttaa tttttgaata
      481 caataatgag tgatgctagc atcaaaatcg ccacctctga ttagtttttt gcttcttcta
      541 ctttatcctc aaaagatgta tcaaatccat tgacttcatt cacttttact gtataattga
      601 catactcacc tgctgtaagc attgtcttta ataactgagc ttctcccatc actccataac
      661 tattttggag ttcggcatcc tttaaatctg gaaatactgt agatgctaca cataattcag
      721 ctacataact gttataatca atttcacttg tatattgacc tgtatgttta ccattgttgc
      781 ctataacttt cactctttta gtgcattttc ttcttaatga ttcatcttct tcagatgata
      841 aaactttaag ttcccattct actggttttc cattctcatc tacgaatcta tcacttgcca
      901 catattttac attatcaacc tttattgcat tttgacttaa aaaagcactt aaattactca
      961 tattattcta atctcctttt attttaattt ttcatataaa aaatacacat atataattta
     1021 taaatgtgta ttttactcca ttcctgccaa taaattaaat ttttctacta attcccaatc
     1081 ctcaaaagtg aaatccatat cttcatctaa atactcacca tcagcatcaa atttagtaat
     1141 tattccactg tccatattac aatctttaag tactactgtc tgtcttccta cagcagatgt
     1201 agggtcttca tttgtaactt gtatgtcaaa ataaatatcc tcaccagttt ctttatatct
     1261 gtaaagtaat tctctaaaaa tagaagtatt ataatgaaat gttgcacttc cagtatttgt
     1321 actcccagtt gttttatttc cctttgttgt tcttcctaga attggaactt cacttttatt
     1381 tttttccatt ttagcctcta aatctatagc ttgcatgaag ttatatcttt tgccttctat
     1441 agttataaaa cattcagctt tctttgcact aactgtatct ttagcattta ttgtttgagc
     1501 catattatca ctctcctctc taactaactg aaacagtcat ataaagctta ctcatagcat
     1561 ttattacctt aacagcatca gatactatga cagttttctt atcatttcca agctctacac
     1621 taacatcatc agttttaaaa tcttctattg cccttacatt ctctaattct ttatggtgtt
     1681 taacaacatc attccagaaa cttattcttc ctgccttatc attcggaact ttacctaaat
     1741 acttttcatt aaataaagtt gcaatatcat tagcaatttg gtcaagtact ctaacacttt
     1801 ggttacttga aaaatcgtca tttttatcgt ctgtaaatga tacaaaagta tttatatcct
     1861 ctaacacatg aacttcatct cccaccttgt ggaatataaa tttaccagtt tttaaagctt
     1921 cttcaagttg tatttgtgta taattaacat caacatcaaa ctcaccatca tactttttat
     1981 tagtattaga tttatttata tcgcatcctg ctatagctcc agccgcccaa taaattaaac
     2041 tagattctac taatccaata tctttaatct tattttctac agacactaca ccttcataat
     2101 ctgcatcact tttcttatat agtactgttt gaaacttagc tcctacctta tctctcattc
     2161 tctttgtaaa ttctacaaat aaacttttaa tttctgttgt tgtagccaaa catcctaaag
     2221 cattaaagct atagctttct attttatcca agaaagcttg gtactctgct cctgtcacag
     2281 cttcgccatt agttccacca gtaaatacca gccctgcact tgcttctagt gttgcatcct
     2341 tcttccaagt gatatagtca ttatcttgta agtctgtaat cactttagct atttgagtat
     2401 ctaccttctt attatctaaa agtgttacaa catcaaactt agcattatca tctatatttg
     2461 ttgtaactgt tacttttaag tcatttcctc tgataccact atattttgct gtggctatag
     2521 tacaactggc tttaacgcct ttatttaatt tataaaaata tcccaacctt atatttttga
     2581 ataaatctct caaacctttc agcttctcat gagtataatc atatccaaaa tacttcactg
     2641 aatacttctc aaaatcatca ctggttactt gaaatacgtc ttcatctatg ccccaatcta
     2701 actctaaagg tattgcaaca atacctctat ccgataatga actggttgcc ctcttagctg
     2761 agataaaatt tatatagcta cctggtaata ttttattctg tgttacaaat gttcctccac
     2821 ctaaagccat ctaactcact cctttcataa atttatttat tctatcctct acttctgaga
     2881 aggaatataa atcattttct tttaaaattg catttaataa atcttttcta tttacatact
     2941 tcttagagtt aactatctgc tccttagtaa acttgtagtt atcttctttg cttaatgttt
     3001 tactcaaaat tatcacctct cttcaaacca ccgaataact ctactgtatc catcttatct
     3061 gtgtcattac tttttatagt aaagtagtta taatcaacaa agaaatgaag aacattatct
     3121 ataatttcaa agttcatatt tgtacctctg actaagtctc catcaatttc tatatactct
     3181 aattcctcca gtaacatctc agctatctca tttatttcaa atgatttatc attactttta
     3241 ggaaaataat gtacatcaaa agagttcttt tttaatgtcc tgccacttgg ataggatact
     3301 ttgcttggat ttaaaggaac aataaaaaaa caaggttcat ttataccttg ctccacatct
     3361 tcactataaa ttgtataact ctctccaaat gttttatcta atttaataga tatcccatca
     3421 attatattat taagcatcaa atactccttt aagcaatatt aatagttttt tctctataat
     3481 cttatcaact tggctttgta gttccatctc tgaaattgtt aagaaatgtt gtcctttaac
     3541 ccatccctta ccacttttag ttctatgccc aaactcaaca tatgcatttt gttatcctaa
     3601 aggcttttta tcctctagct cttatagttt cctataagtt cggcgtacat catcaacaaa
     3661 ataaacttta tttagttgcc cagcactctt ggagagatta tatttattca ctctctacgc
     3721 tctacggaaa cctatagcct attcgcaatc tataggtttt cctcggtatt ggcatatata
     3781 attaattaaa tatttttcca tgttcttcta tggactatat ttgatataga tgaatatgtt
     3841 acaggatata tttcacttag ttgttttatt gtatatccat cagaatactt ttttctaatt
     3901 tcttttacat cctcaataga taacttagct ctgctttctt caataatctt aactttttct
     3961 ataagattat ttcctactaa tcccttagaa tatctacttc ttaagcaaga atatgaaata
     4021 cctgtttttt cagaaagctc tatcaatgta atttcttttt cttcatgctt tactaatata
     4081 tttgttgacc tatttctaca ttgtgtcttt atatccaccc atctacaatt gcttgattca
     4141 taatttcctt cattatttat tctatcaatt gtaagtttct ctgaatatcc attactcaat
     4201 gcccaatttg caaaattatc atagcaaaac cattcatcac acacaattat attcctttca
     4261 ccatatctat tgtatctttt attattttta tctgtacatc tagatattat acccttccat
     4321 atactgtaaa gtcttttatt ttgaacctta tatttctttt taaattggta tttgtctgtt
     4381 aaatttaatt tatcttgttc tttttttaaa catccacaag attgtactaa gccactagtt
     4441 aaagagtcag ttcttatttc tttgaaattt ccacaatcac acttacataa ccaatatttt
     4501 ctatttcttt taccactttt tatctcttta gaaaatttaa tgactcttag ccttccaaat
     4561 ttttttcctg taatatctaa aaattttgcc ataaaatcac ctcttttata cattataaat
     4621 caagagttat tctatgtcca tattctacct actttataat gtaacactat tacacttagc
     4681 gtttaccgat tttgctgggt tttatatgcc ccattgtgtt aaggcatatt cagtcggatt
     4741 aacaacctct ataatataat tatttccttg tttatacaca ggaagcgacc tagcataagc
     4801 cactccattc catccttgtc gtaagaatcc tgtatcaact ggtgttcttc taattacttt
     4861 cccaagtaat cgtgctgcta attctcttgc tgcatccttg caaaacttat ctaaatcaat
     4921 ctttgtaagc tcctccatct ttttacaaac tcttttaaat tctctaaaat caacactgcc
     4981 ccatctagcc attatgcttt atccttaaat aactcaagta ttatttcttg atgatttgga
     5041 tatatagctg attctccact tcttacatac tctttatcat ttataataag ttttgaacct
     5101 gctttaattt ctatatctgg agatataaag agtttaatag tttgctctag cttagctact
     5161 ttcccttctg tagcagaaac tatattttta tatgaaagtt tacatggttg attttctaat
     5221 acaatcactt ctttattgtt agttcgtttt gttacagggt ctttaattgg ctgatactca
     5281 actatagtac atttatctct atataacatt tctattgctt ttctagtttt acttaccatc
     5341 ttaagcacct aaaggttaat atattattct taccataagt agtaagataa gctattaagc
     5401 tatcaaagcg ttgttctggt gtttgagaac cacttcctat agcaaaatct acctttgtat
     5461 caccttctga tatagacttt tctacagctt caaagttaat gctttctata tctaattgcc
     5521 ccatattttt cttggtaaat aagaactctc caactatcat atcaacttca atttcttcca
     5581 attcaattgg catagttttt atattacaat ctagtttaat aatattttct attttttctc
     5641 ttacaaaacc tattaaccac ttatccccat cttttaatat atatccaaaa ctttcaagtc
     5701 ttttttctat ttcatcaatt atattacttc ccataatttt cacctacttt ttagtaagtt
     5761 tatttttctc tttaagctgt ttattttctt cttctaaaga ctcaactttt gaccttaaaa
     5821 tattattttc agctattaaa tcttttacat ttaatgactt gccatacttt actgccttac
     5881 cagtttcatc tatcaaatca tatcccatct ctaagaaatc atctatttta cactcttcta
     5941 tagttaatat tctatttaat ttccttactt gtgccattat actccagctc cttcaacaac
     6001 aaattgtatt gcatcagctt ttttatttaa tataaataca tcctcaaaac tttcttcaaa
     6061 gtagaagtat tttccctctg taactgctgt tggttcgtct aacttagaga actgataaga
     6121 aacaggtgta attattgcac ttgggtgaac taaggacata aatatttgtt tagctcctgc
     6181 tcctactttc catccagttg taaaatcata tgcagttttc attagattag atggtacttt
     6241 aattatttta actgtgtcaa tatctgtggt ttgtctattt aaagaagttc caccatcttt
     6301 tatgtttact gttctttgta tctcttttgc atttttgata agtgtattta ctactggagt
     6361 aacatacaat attcttccat tttcaggtac tctagcttct gtcatttttt ccattaactt
     6421 atcaaatact tctaatacgt ttgctgttgt aagaacagtt gtatctgctg tgttacctaa
     6481 tgcggtccaa tcagcatata ttttagatat acaataagca tccatctctg gaaacttttg
     6541 ttcctcatta tatacttttg ttatattgcc tattgaagcc acataattag tttggtttat
     6601 atctgctgga tgaaccaatg ttgaccattt cctttgatta gttaatacct taggttccca
     6661 agcattatca tagtttcttt gagctactgc tattgtatct ctgtttgaat ctactcttcc
     6721 agttgtagat atagttggta tttctattgt tttagaacca gtccatctat atcttccatt
     6781 atttggtgtt gcatacaaat ccccgaagtt taaagtataa ggatatgctt gtgctaaaac
     6841 atttgaatat tcttttgcat aatttagtgc tgccatttta tttcctccta tttattatta
     6901 ttttcatgag gtcttacccc agtaaaatta aaaccaaaat catttatctt aggctcttgc
     6961 cctggtgtta tagtatctat tttaggttct tcaccttcta gtgttgcatt aaacaaataa
     7021 tctttatcct gtttcaaagg atttatttgc tcttcaaaag ctttttgtct gtctttacta
     7081 tttcttagtg cttctatatc taaatgagct tttaatgcta tttcatctct acatttaaca
     7141 gatttaaaag catcacctaa ccagtaatta aaatcttttt cttcaatttc ttttttgtag
     7201 gtttcttcca aagttttctt atcagtttca taagttgttt ttagattctc tacatcttct
     7261 tttgtcatac ctccttcaaa ctttttaata gtttcattag ctgtattaag ttgtgtttca
     7321 agatttgtat aatcttcttt agtaattgta gtctctttta ttttcttttc tatagatttt
     7381 tgaagagaag ctacatcaat tttgttatcc tctactttta ttccttctag caattctttt
     7441 aaccaatcca ttttaaattt ctcctttcat tttttacaaa ataaaagcat ctacttattt
     7501 ttaagtacat gcttagtcat tccttattta tattttcgat agattctatt tcattttcat
     7561 aaacttcaat tccataacca tccctagcta ttgatatact tgctatttct ggttcattat
     7621 ctaaagcttg tgtatatcca tcacactttc ctcttattat ttgcttatct acacaagtta
     7681 tttgaacatt tttccctaca tattcccata atttcatttt atttttcctc cttataaagc
     7741 tggtactata tgtgttccag ttttggaata atgtatctta aacttatttg taagagtttt
     7801 ttcacccgta atattattaa cattgacccc tatattctta tcaacttcta taagttcttt
     7861 tttatcccat tctccacttc gattaaattt tatgattcca ttgccagcat gcttattcac
     7921 aagttcttga gcttcttctt ttgttatagt taaatagctt cttccttcta tataattatt
     7981 atgctctttt aaatgttttc cttgtttccc atcatgaata tttaaattat atttaccatt
     8041 tttaatatct tcttttatgc tatctattat agcactattt tttatttcta agatactatt
     8101 atgtttaaca tacttctcat accactcatt atacttcata ctagatggta cataatatgt
     8161 ttttccatct tctcctcttg ctgctctata gccttcttca tcctcgaacc aaggagctgt
     8221 tgttgtccta caacgacaat gaaatggtgg agctgttatt ccaacttgat aatctttcat
     8281 atcaaatatt tttccatcca gctctctaca tatatttgaa gttcttaaat ctaatgtagc
     8341 aataatctca tatttctcta catctaaatc actaaaacaa tcttttctac ttgctgatgc
     8401 aaagaaagct gattcagtca ttatcaaatt cttagcttgt gacttagata cattaaatct
     8461 tttatcaaag tcatttacta aattttttgg attttcacct ctaataattg attgagttag
     8521 cttagtatgt agttcattta ctaaagtagg tctatactta ccccaaattc tttcactaaa
     8581 gtttaatcca tctgttgccc atggtttaga gataatttta tttattctat tagtatcaag
     8641 actcattaaa ctccaaccaa cgtttac
//
LOCUS       DAKBNN010000160         8657 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000160 DAKBNN010000000
VERSION     DAKBNN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.161"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            85..1575
                     /locus_tag="OUB02_002968"
     CDS             85..1575
                     /locus_tag="OUB02_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCT8886704.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1855..3351
                     /locus_tag="OUB02_002969"
     CDS             1855..3351
                     /locus_tag="OUB02_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCT8886705.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3485..4411
                     /locus_tag="OUB02_002970"
     CDS             3485..4411
                     /locus_tag="OUB02_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8886706.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4529..4720
                     /locus_tag="OUB02_002971"
     CDS             4529..4720
                     /locus_tag="OUB02_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886707.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4943..5293
                     /locus_tag="OUB02_002972"
     CDS             4943..5293
                     /locus_tag="OUB02_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886708.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            5556..7061
                     /locus_tag="OUB02_002973"
     CDS             5556..7061
                     /locus_tag="OUB02_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCT8886709.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            7451..8548
                     /gene="dinB"
                     /locus_tag="OUB02_002974"
     CDS             7451..8548
                     /gene="dinB"
                     /locus_tag="OUB02_002974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCT8886710.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
BASE COUNT         3429 a          880 c         1609 g         2739 t
ORIGIN      
        1 ataatatttt atgtatatat taataaaagg acatattgtg ttataatggt atcgtataaa
       61 atcatttacg ggggtggtat taaaatgttt caatttgaaa cacaaataca caaattaaga
      121 catgatgttt taactgcaat agctagactt ggaaaagaag ataaccttac cagagaagca
      181 atggataata ttcagtatga aataataaaa ggtgagaaac ctacatacag atgctgtgtt
      241 tataaagaaa gagctataat atctgagaga gtaaatgtaa gtatggggtt acaaccaggt
      301 caagataaga tagatttaga tacaatggat atagaagaag atgaacaaat tatatatatt
      361 ttggaagctg catgtgacag ttgtccaata aatgaattta cagtaacaga tatttgtaga
      421 ggatgtctag cacatagatg taaagaagct tgtaaatttg gagctataac acatgtcgga
      481 ggaatggcct atataaatca tgaattatgt aaggcttgtg gaatgtgtaa aaaagcttgt
      541 caatatgatg ccatctcaga ggttgttaga ccatgtaaga gtgtttgtcc aactaatgcc
      601 ttaggatttg atagagaaaa tatgaaagct atgatacatg aggataaatg tttaaactgt
      661 ggagcatgta tgtcagcatg tccatttgga gcaatatctg ataagagttt gatagctcct
      721 gtagctagaa agttagttca aaaagagaaa atgtatgcgg tggtagctcc agctataaca
      781 ggtcaagttg aagcaaatgt tacatatggt caaataaaaa atgctataaa atcactagga
      841 tttgttgatg tattagaagc agcttgtgga gcagatgcag ttacagttca tgaaagctta
      901 gaatttgctg aaagaatgga aaagggagaa gcttttatga caaattcttg ttgtccagga
      961 tttgttggtt atatagaaaa aacataccca aatcatgtag gaaaaatatc ttctacagtg
     1021 tctcctatga tagctagtgg tagatttata aaaagtcatg aaccagatgc taaagttgta
     1081 ttcataggac catgtacagc caaaaagagt gaggcagcta agaaagaatt aaaaggagct
     1141 gttgattatg tacttacttt tgaagaaata atggcattat ttgaggcatt tgatatagac
     1201 ttggctaagt gttcaaatga agatatagat gatgcatcaa tttttggaag aggttttgga
     1261 gctgctggtg gtcttacaaa agctatagaa aattatcttg ctgaaaaagg tattggtgtt
     1321 aattttgaac ctgtaaagat ttctggttca agagaaatca agaaaactat gactatggct
     1381 aatttaggaa agctagaagg taattttata gaaggaatga tgtgtgaagg tggttgtata
     1441 aatggttctg gtaaattctt atcaggtgca aaggttaaag ccacttttga tagaaaaaat
     1501 tctcaatcaa ctaagaaaag tgtcttagca aatacaaaga taaaagattt tagtaatata
     1561 aatttagaaa aataaagata atacaaagca acaaaaatac atattgttaa tagagttgtt
     1621 ctaagttgaa gtttaacttt tggacaactc tatttttatt tatatattta attattgaac
     1681 aacaaaaata aaaattatat acacttaaaa taaaaattac atatataata ttttgtatat
     1741 aacttaacaa agtatatgcc tatgttataa taatttatag aaataaataa aaaatacaat
     1801 atacataata gtagaaatat agcaaaaagg tgtgatttat agggaggatt acttatgttt
     1861 gaatttgaaa cgcaactaaa aaggttaaaa catgaagttt taacagcagt agcaagacta
     1921 tctattgaag aaaatttaac aaaagaaaat atagaaaaaa ttccatatga aataattaaa
     1981 ggagaaaagg cgagatatag atgttgtgtt tataaagaga gagaagtagt tcttgaaagg
     2041 gcgcaacttg cattaagttt aactccaaat agtaaatatg gagatataaa tccagattca
     2101 gttagtttgg acacagatga gcaaattata tatatagttg aagcagcatg tgatagatgt
     2161 ccaataaatg aatttacagt tacagagctt tgtagagggt gtttggctca tagatgtaag
     2221 gaagcatgta aatttggggc aatttcttat ataaatggga gagcatatat aaatcatgat
     2281 ttatgtaagg cttgtggaat gtgcaaaagt tcatgtcaat atgatgctat atcagaagtt
     2341 gtaagacctt gtaaaagcgt ttgtccaaca ggagctttgg attttaatag aaacactatg
     2401 aaagcaatga tacatgaaga taattgtata aattgtggag catgtatgtc agcatgtcct
     2461 tttggagcaa tatctgataa gagtttgata gcacctgttg caaaaaaact tgcaaagaaa
     2521 gaaaatatgt atgcaatagt agctccagct ataacaggtc aaatagatag taatattaca
     2581 tatggtcagt taaaaaatgc tataaaatca ctgggatttg tagatatgat agaagcttcc
     2641 tgtggagcag atgcagttac agttcatgaa agcaatgaac ttattgaaag aataaaatca
     2701 ggagataatt acatgacaaa ttcatgttgt ccgggatttt ttaagtatat agagaagatg
     2761 ttcccagatg aaaaagatag aatatcatct acagtgtctc ctatggtagc aacaggaaga
     2821 ttagtaaaaa aaatagattc tgatgctaaa gttgtattta taggtccatg tacagctaaa
     2881 aagagtgagg caacagagaa aagtttaaaa gattcagtag attatgtact tacttttgaa
     2941 gaactaatgg cattatttga agcatttaat atagatttat caaaatgttc aaatgaagat
     3001 ataaatgatg gttcgatttt tggaagggga tttggagcat ctggtggact tgccaaatca
     3061 gtacagaact atattgctga aaaggggctt agttttgatt ttgaacctat aaaagtatct
     3121 ggtccaagac aaattaaaaa gacaatgaca atggctaaat tgggaagatt agaaggtaat
     3181 tttatagaag gaatgatgtg tgaagaaggt tgtattaatg gagcaggtaa gtttttatca
     3241 ggtgtaaaag ctaaaaatac ttttgataga aaaaatggac aatctactaa aaagagtgtt
     3301 ttatctaatg ataagattaa agaatttgaa gatgtaaatt tagaaagata attgttttgg
     3361 tacagtcatt tttttattat gccacaaata tgcctaaaat ttgccctttt tatatgtgta
     3421 ttttacagag ttaaggttgc tatactataa atatgtaata aataaaattt ataggagctt
     3481 ttggatgagg aaaatagtat taaaagatat cctgttttta gttactacat tatctataat
     3541 agtaatatta ttgcttgtaa caaatataac tactaatagt aagataaaat caacaagtga
     3601 atcttattta aaaagtgaca aaaatagtgt gaggcaaaat ggtatccaga ggagcacaaa
     3661 gagtagcaca aaaaatactg catttaagac tactatacta aacactggaa agtctgattg
     3721 catattggtt gagataggta acaaagtcat aatgattgat acaggagaag ataaaaatgg
     3781 aaaacaaata gtggataggt taaaagaaaa aggaataaat acacttgact atttaatttt
     3841 aactcattta gataaggacc atataggtgg agtagatagt gtgttatcta gtgttaaagt
     3901 aaagaatatt atacaagcaa attataagaa agatagtaag caatatgatg aatatattga
     3961 ctcacttaaa aaagctgata tagaaccagt gctactaaaa gataatatga atattgtaat
     4021 caatagtgca gaaataaaca tacatccggc ttcaaaaagc aagtacgaga gttcaaatga
     4081 ttattcaata atcactaata taagctatgg agcacataag tttttatttg caggagatgc
     4141 agaggaagaa agattgtcag agtttataaa tggaaatact ttaaaatatg attttgttaa
     4201 aatgcctcat catggaagat atgacaaatt aactgaaaca tttttagagt ctatttcacc
     4261 ccaatatgct gttattacat gttcagagaa aaaagaacca gaggaaggtg ttttaaaaat
     4321 attggaaaga ttgaatataa aaaccttttt gacttctaat ggagaggtag ttatcaatag
     4381 tgatggtaag acattgagtg tgaatcaata atttttttaa aaatatctaa aaaaatttaa
     4441 aaaaatcatt agcaaatatt accccttaaa catagtatat tttaggagct gaaactccac
     4501 atacaaaatg actaggaggt aacatattat gttggataga ttttttggtg aaggcggatt
     4561 tggtggatgt gcttggtgga taataatatt attcttctta ttcttagcat tccaagattg
     4621 ttgggctgaa atggacttat gtgcatggat acctttctta atattattat taatactttg
     4681 tgaatgtggt gacgtttttg atggaggatg tggatgctaa aaaaatcaat ttagatgatt
     4741 taataaagct tatggtgggt ataaataaaa taccaaacca atcacaatct agtgtttgaa
     4801 ggtagtcgga aatcccccgc taccttcttt tttttattgt taaaatttat ttttgaatta
     4861 tttcatttac atataataat ttttacgata aagtctgata taattaaaaa gaaaataaaa
     4921 tttatttata ggagatgtaa aaatggctat agtggataag attttcaatg agataaaact
     4981 tatggttgca agacttttat tgggaaaaaa gtacagtgaa tatgaagaaa aagatttagt
     5041 gtataataca gaagaagaag ttatacttat aactttaaaa agattagttt ttcagggaaa
     5101 tgtaaacgaa gcagaagaaa ttttatttga taaagctaag agtgtaaata gtgaaaatat
     5161 gcagtatata gcaatagaat tttatactat gcttatggaa aagactgatg aagaattaga
     5221 agctatgaat ttttctaaac aagaagttta tcaaggtata gaagatatta gaaaagtatt
     5281 gaatttagaa taaaagatat atgaaaatat tgaaataaat taatgctaag gtgagaatat
     5341 tatacttata tataaaaata taatattctt attttattag ttatatttag gtatatctta
     5401 tttaccataa aactatcttg aaaaaaatta atcttaattg tataatatta ctttgagtga
     5461 aaaagtatta acattttttt ataagatgtt ttactaaaaa aatatatata aatatactgt
     5521 tatcaataag aaatttaatt tagtgaggtg ttaagatgaa agtaggattt gaccacaaaa
     5581 aatatttaga agagcagtct aaatatatct tagaaagagt aaataatttt gacaaacttt
     5641 atttagagtt tggaggaaaa ttgatgggag accttcatgc aaaaagagta cttccaggat
     5701 ttgatgaaaa tgcaaagata aaagtattac aacatatgaa agaaaaagtt gaagttgtaa
     5761 tttgtgtcta tgcaggagat atagaaagaa acaaaataag aggagacttt ggtatcactt
     5821 atgatatgga agttttaaga ttaatagatg atttaagagg ttatgaacta gatgtaaata
     5881 gtgtagtaat tactagattt gaaggacaac ctgctacaac tgtatttata aataaattag
     5941 aacgtagagg aattaaagtt tataagcatt atccaacaaa gggatatcca tcagatgttg
     6001 agactatagt tagtgatgag ggatatggag caaatgaata tatagaaact acaaagccaa
     6061 tagtagtagt cacagcacca ggacctaata gtggaaaact tggtacttgt ctaagtcagt
     6121 tgtatcatga aaataaaaga ggtaatgtag ttggatattc taagtttgag acattcccag
     6181 tttggaatgt acctgtaaaa catcctttga atatagctta tgaagcagct acagttgatt
     6241 taaaagatgt gaatatgata gattcattcc accttgaaac gtatggtgaa atgtcagtaa
     6301 actacaatag agatttggaa ttatttccag tgcttaaaaa aataatagaa aagataactg
     6361 gaaaagaatc aattttccaa tctccaactg atatgggtgt taatagagtt ggctttggaa
     6421 taatagatga tgaagtaata agaaaagctt ctatagaaga aataataaga agatacttta
     6481 aaactgcctg tgagtataaa aagggacaag ttgataaagg tgcttatgat agaatgaaaa
     6541 tgattatgga agagcttaac ttaaagccag aagatagaaa tgttgtatta cctgctagaa
     6601 actacagtaa taaattgaaa gaaagtgccg acaagaatga tacttgtcct gtagttgcat
     6661 tagaattaga agatggtaca atattaactg gtaagggttc tgatttaatg aatggaactg
     6721 cagcagtagt attaaatgcc attaaacatt tagccaatat atctgatgat atgcacttaa
     6781 tatcacctgt aatattagaa cctatcataa acttaaaaac aaagacttta gctagtagaa
     6841 atgtttcact tagttgccaa gaggtgttaa ctgcacttag tatttgtgct gtaactaatc
     6901 caactgcaca atttgctatg gaaaagttag cattattaaa agggtgtcaa gctcattcta
     6961 ctactatatt aaatagagat gatgaacaaa catttagaaa gcttggtata gatgtaactt
     7021 gtgacccaga atatccttca caaaatcttt actatagtta aaattatata aaaaaatatg
     7081 ggctgtttta aagtaatagt ttgagcagtc cattacaatt ataataaaat cagtaggctg
     7141 tcttgtataa gacagtcttt tgtttttaaa acatcccata aattaaatat atatactgtt
     7201 atccataagg aattttaaat ttagttcctt ggaataccta ttgtaaagct tttatatagt
     7261 ttttgcaagt gtttttaagg attttttata aattatattg aagttaatgg attatagatg
     7321 gttttattta tggaaaatat gaatatataa agaaaataat gaaaaaatac caaaaagaaa
     7381 cataagtttg gtataattaa aaagtaatat aaataaaatt aatctattta tactttaaaa
     7441 ggtgataata atgaaacatt atagaaaaat aattcatata gatatggacg ctttttatgc
     7501 atctatagaa caaagagata ataaaaaatt aaaaggaaga cctgttattg tcggtggaaa
     7561 tccacaaagc cgaggtgtgg ttgcaacgtg ttcttatgaa gctagaaaat ttggtatcca
     7621 ttcagctatg ccatcagctg tagcttacaa taggtgtcca tatgcagttt ttgtaagacc
     7681 tcgatttgat gtttataaga gtgtgtcaga gcaaataaga gatatatttt atagatacac
     7741 ggatttagtt gaaccattat ctttagatga agcttactta gatgtaacaa aaaataaaaa
     7801 aaatattgat tcatcaattg agatagcaaa gcaaataaaa aaagatatat ttagagaagt
     7861 tggtcttact tcttcagctg gtgtgtcata caataagttt cttgctaaga ttgcatcaga
     7921 cttaagaaag ccaaatgggt taactgtaat aaccgaagaa aatgcccagg actttttaga
     7981 taaattacct gtgaataaat tttttggtgt tggaaaagtt acttcaaata ctcttaaaaa
     8041 tttaggtata aaaactggat atgatttaag gtgtttaaat ttatttgaac ttgaaaatat
     8101 attcaaaaaa agaggttatg aattatataa atttgctaga ggaatagacg atagaccagt
     8161 ggaaccaaat agggttagaa aatctgttgg ggctgagact acattaagtc ataatttgga
     8221 tattgatgaa gaagaaacac gcaacattct tgatgaactt tgtgaagaag tatgtcatag
     8281 attaaaaaac tctgagaagt ttggaaaaac attaacatta aaaattaaat atgaggactt
     8341 tacaaagata actagaagcc ttagtttaga acactatatt gacgaatata atgatataag
     8401 aagtggtgta gataatttgt tgagaaatgt tgaggttaat ggaaaacaaa ttaggctatt
     8461 gggagttaca atatcaaacc taagtgataa aaaagaaact tataaagata ttacattatt
     8521 tgaatacatg gatagtatcc aaatgtgagt tttgtttaaa tgaaataaca ctagctttaa
     8581 tactatataa ttgaggctag tgttatttta tgaatatttt tatatttttt gttttttgac
     8641 attatatatt atatact
//
LOCUS       DAKBNN010000161         8562 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000161 DAKBNN010000000
VERSION     DAKBNN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.162"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            483..1643
                     /locus_tag="OUB02_002975"
     CDS             483..1643
                     /locus_tag="OUB02_002975"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCT8886711.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1645..2775
                     /gene="glgD"
                     /locus_tag="OUB02_002976"
     CDS             1645..2775
                     /gene="glgD"
                     /locus_tag="OUB02_002976"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HCT8886712.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2804..4246
                     /gene="glgA"
                     /locus_tag="OUB02_002977"
     CDS             2804..4246
                     /gene="glgA"
                     /locus_tag="OUB02_002977"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCT8886713.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4260..6680
                     /locus_tag="OUB02_002978"
     CDS             4260..6680
                     /locus_tag="OUB02_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HCT8886714.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMIKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6685..8550
                     /locus_tag="OUB02_002979"
     CDS             6685..8550
                     /locus_tag="OUB02_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HCT8886715.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDS
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
BASE COUNT         3448 a          824 c         1572 g         2718 t
ORIGIN      
        1 aatactaaga aaactaataa ataatttaaa agtttatatc cttttgttta aattatagac
       61 aaagaagtat caattaaaaa ataaagttga ttaataatta cttgattttc cttggataat
      121 tatttaacaa atattagatt atattttgct attacgaatt taacaaatta aaaaataagc
      181 atgttcatat gacacaagag atttataaaa aatgttttta tatttataaa ataaaatttg
      241 tattattttt taaccgataa ttgaatatta ataaatacta tagtgcttat ggaaaataat
      301 gtatacgtcg gtaagttcat tatggggtat acattatatc aattgtatag ttgatattat
      361 tttattttta ttaaggtatt atttaagcaa catttataca cgacatttat tgactgataa
      421 agtttataaa agtaaaaatg caattataaa tattaattac tagatgaggg gagtgtcatt
      481 aaatgaaaaa agagatgtta gctatgattt tggcaggagg gcaaggttca agattaggtg
      541 tttttacaaa aagaattgcc aaaccagctg tatcatttgg aggtaagtat agaattatag
      601 attttgtgtt aagcaattgt tcaaactctg gtattgatac tgtaggtgtt ttaactcaat
      661 atagaccgtt gatattaaat tcacatatag gaatgggaag tcattgggat ttggacagaa
      721 taaatggtgg tgtctatgtt ttacaaccat ttatgaatga aaaagaagga aattggtata
      781 atggtacagc acatgcgata tatcaaaata tggactttgt agatacctat aatccagaat
      841 atgtattgat attatctggt gaccatatat acaagatgga ttatagcaaa atgttaaaat
      901 ttcataagga aaaaggctct aaagctacaa tagcagtgat tgaagttcct tgggatgaag
      961 cttcaagatt tgggataatg aatacaaatg aagattcaag tatctatgaa tttgaagaaa
     1021 aaccaagtga acctaagagc aatttggcat ctatgggagt atacatattt gattggaaaa
     1081 tgttgagaaa ttattttaag gaagcagaaa aaaatccaga aataaattat gatgattttg
     1141 gaaaaaattt aatacctaaa atgttagaag ataatgttgg aatgtatgct tatccattta
     1201 aaggatactg gagagatgta ggaactatac aaagtctatg ggatgctaat atggatataa
     1261 taaaatctcc agaaacactt gatttagcag accctaaatg gaagatatat acaaatacta
     1321 tggcaatgcc tccacaatat ataggtaaaa atgcaaatgt tcatagatca atgatagctg
     1381 atggttgtag aattttaggt gaagtaggta actctgtatt atcacatgga gttgttgttg
     1441 gaaaaggaag taaagtaatt gattcagtaa taatgccaaa tgttgtaata ggtgaaaatg
     1501 taactataga aaaagctatg ataggtgagt gtgcaactat aaatgataat gttcagataa
     1561 agaatgtaaa taatgaaata aatgtagttt ctgagtatga aaatatcgaa cctagatgcg
     1621 tattaataga aggaggtctt taagatgaga aatgaatgtt tagggataat aaatttaaac
     1681 aaaaaaggag acccagcaat aaacaaactt aactatggaa gaccaatagc atctacacca
     1741 atagctggaa gatatagaat aatagacttt gctttatcta atatgataaa ttcaggaatc
     1801 actaaagttg gaatattcgc aaaagaaaaa tacagatcat taacagacca tataggtagt
     1861 ggtaaagatt gggatttaag tagaaaaaaa ggtggcctat caatttttag tccagaaaac
     1921 actaagtata gaaatactta ctcacataga gaaggtgata tatatactat cttagctaat
     1981 ctagattata tagaaaagag tgaagaagaa tatattttaa tagctccaag ctatatgcta
     2041 tgcaatttaa attattctca agcattggaa taccataaaa aatctcataa tgatataact
     2101 ataatatata aaaatgtaaa taatgcaaat aaagattttg ctggtaactt aactctaaat
     2161 ttagatagta acaatagggt aataaatgta ggaaataatt taggtaagtt ccctagagca
     2221 aacatatgta tggagacata tattatgaag cgtgaagatt ttgttgaatg tatatataac
     2281 attgtaaata aaggcaatta ttgttattta gaagaattta ttattgaaga agcagaaaac
     2341 atgaaaatag gagcctatga atatacagga tatctaaaat gtgtaaattc tgttgaatca
     2401 tattttgaaa tgagcaaaga tttattaaat ttagaagttg cagatgaatt attatattct
     2461 gaaagaaaga tatttacaaa agaaaagaat gaatcaccta caatttatac agatagtgct
     2521 aaagtagaaa atagctttat agcatctggt tgtttaatag aaggtactgt aaaagatagt
     2581 atcatattta gaaaggtaaa tgttgaaaaa ggaacagtta tagagaactc aatagtaatg
     2641 caaaattgtg taataaagtc aaatgcaaaa ctatataatg caatattaga taaaaatacg
     2701 agtgtatcaa atgggaaaga attaagagga gatgaaaaat atcctcttgt agtggaaaag
     2761 aatacaaata tataaataaa ttttttagaa gggggaatga ctaatgaagg ttttttatgt
     2821 aacagcagag tgttggccct ttgcaaaaac tggaggatta ggagatgttt cttatgcatt
     2881 gcctaaagaa ttaaaaaaag aaggtgtaga tgtaagggta ataatgccca aatattcaac
     2941 tataccaagt tatttaaaag atcaactaaa agaaatagct gtgtttagtg tacgtgttgg
     3001 atggagaaat caatactgtg gtcttttaga aatggaatta gatggtgtaa aattttactt
     3061 tatagataat gaattttatt ttagaagaga agatgaaaga aagagtatct atggatatgg
     3121 agatgatgca gaaagatata ctttctttac agatgcagtt ttagaagcta taagtagaat
     3181 tgatttttat ccagatgtaa tacatataaa tgattggcat acagggatgt taccattaat
     3241 tctaaaagaa agatatgcaa cccttgaggg gtataaaaat ataaaaacta tgtatactat
     3301 acataatctc caatatcaag gtgtatttga taagcatgtt ttatatgata tattggattt
     3361 accacaaaaa tactttgata atggagatat agagtattat ggctcaataa attttatgaa
     3421 agctggtata aattttgcag ataaaattat aactgttagc cctacttatg caaatgaaat
     3481 acaaacatct ttttatggcg aacaattaga tggattactt agaaaagagt caggaaaatt
     3541 aaaaggtata ttaaatggta tagattatga tttaaatgac cctgcaaaag ataaagatat
     3601 atttgttcat tatgatgtag actcaattga caaaaaagta gaaaataaat tgagattaca
     3661 agatatattg ggactaaaaa aagattcatc tattccgttg ataggtatag tttcgagatt
     3721 agtttctcaa aaaggatttg atttaatagc atacatgatg ccagaactta tgagagaaga
     3781 tttacagatt gtcgttttag gaactggaga acatcaatat caatcaatgt tcaactatta
     3841 cgattctaat ttttcagata aagtatcagc tagaattact tttaatgcat cattggctca
     3901 acagatatat gctgcaagtg atatgttctt aatgccatca ttatttgagc cttgtggtat
     3961 aggtcaaatg ttagctatga gatatggttc tttaccaata gttagagaga ctggagggct
     4021 tagagataca gtaacacctt ataataaatt tactggagaa ggcaatggat ttagttttaa
     4081 aaattacaat gctcatgaga tgtttttctg tttgaaaaat gcaatcaaag tatttaagga
     4141 caaagaaaaa tggattaaat tagttgaaaa tgcaatgaaa acagataata gttggaaaaa
     4201 atcagctaag gagtatatag aaacatatag agacatatgt gattaaagga gtggtttttt
     4261 tggtaacaat aagcaaaaag aaattcaagc aaaaatttga agtaaaaatg tatagcttat
     4321 atgcacaatc tatcaaggaa gctactgatg aacaattgtt aaatgtatta tgtagtctat
     4381 tgaaagatga gatagcaaaa aaatgggttg ctacaaagtt agataagaaa aaagaagttt
     4441 attattttag tttagaattt ttaataggga gacagttaaa atctaattta ttaaatttaa
     4501 atattgaaga agaagttaga gaaggtctat ctgaattagg aatcaacttg gatgatttaa
     4561 ttgaagctga agtagaccca gccttaggaa atggtggact tggtagatta gcagcttgct
     4621 ttttagattc tatggcatct ttgaacataa gtggtcaagg atatggaata aggtataaat
     4681 atggtctgtt tgaacaaaaa tttgtaaatg gttatcaggt agaagtgcct gataactggc
     4741 ttacagaagg acgatatgca tgggaaactg taagaccaaa tgaagcaaca atggtgaagt
     4801 ttggtggtga ggtagaatta atcaaggaag gtagtcattt aaaagtaatt cataaaaact
     4861 accttcctgt aatggcaatg ccttatgata taccaataat cggataccaa aaccaatgta
     4921 taaatacttt aagattgttt aagagtgaaa taccaaagag agattttggt gaacttactt
     4981 caaatgctct aaattattct ggtagctatg aagaggcttt aaaacataag tattatactg
     5041 aggaaatatc acaggtatta tatcctgatg attcaaatta tgcaggtaag ttgttgaggt
     5101 tgaaacaaga atactttttt gtaagtgcag ggatacaaga tataataaga aaatataaaa
     5161 agaacaaatt aaatattaat aatttattcg ataaggtagc aattcatata aatgatacac
     5221 atcctacctt atgcatacca gaacttatga gaattttgct tgatgaagaa aatttatctt
     5281 gggatgaagc atgggaaata actaaaaaaa cagtgtccta tactaatcat actataatgt
     5341 cagaagcaat ggaaaaatgg cctgtaagta tgattaaaga actattacca agaatttata
     5401 tgataataga agaaataaat agaagatatg tagaggaatt aaataataaa ggatatgagc
     5461 aggatagaat aaaaagaatg agtataatag actgtgacaa tatcaatatg gcaaacttat
     5521 gtattgtcac aagtcatagt gtaaatggag ttgctaagct tcatactcat attcttgaaa
     5581 cagaggtatt aaaagatttc tatcaagatg aaccaaataa atttaataat aagactaatg
     5641 gaattgcaca taggcgatgg cttataagtt caaatcctca acttagcaat ttaattacag
     5701 atttaattgg tgattcatgg aagaaagata cattacaatt aaaaaatatt gaaaaattta
     5761 aaaatgattc ctctgtttta caaagacttg atgatataaa atacaataat aaagcaaatc
     5821 tagcaaagtt tataaaagat aaatacgatt taaatgtaga cccaagttcg atatttgatg
     5881 ttcaagttaa gagattgcat gcgtataaaa gacagttact gaatatattt aatgtacttc
     5941 atatgtatca tgaattactt gataatccta atttgaatct tgacccaaga acttttatat
     6001 ttggcgcaaa agcagcacca ggatattatt tggctaaatg tataattaaa tttataaatt
     6061 ctgttgcaag tactataaat aatgatgtta gagtaaagga caagttaaaa gtagtatttt
     6121 tagagaatta tggagtatca ttagcagaaa taataatacc agcagcaaat gttagtgagc
     6181 aaatttcaac tacaactaag gaagcatctg gaacaagtaa tatgaagttt atgatgaatg
     6241 gtgctataac tttggctaca cttgacggtg caaatgtaga gatatgtgaa caggttggta
     6301 aagaaaatat gtttttattt ggacttagtg cagaacaagt actaaattat aataaatatg
     6361 gtggatattc ttcacttgac ctatatcatt ctaatatgga tataaaaagg gtagtagatg
     6421 atttaataaa tggatttatt cctaatcttg gcgaagaagg aagaagtatt tataattcac
     6481 tcactactta caatgatgag tattttgtat tgagagattt tgaaaattat ggtcaagctc
     6541 aagcagatat aaacaggtta tatagagata aagaaaaatg gaataaaatg tctttagtta
     6601 atatagctaa ctctggattt ttttcttctg atagaacaat ttctgagtat gcaaaagaca
     6661 tttggtttaa aagggtgtga ttaaatgcaa agtgtaatag aatataactc ttgggataaa
     6721 aattttaagg ctccgtttgg agctttaaaa tttgatgagg aattgaccat ttgtgttaaa
     6781 gtaaatgagg gatacaatat caagtctatt tccctagaaa taaatagaga agaagaaatg
     6841 agaacaataa ctttaaatga agaattagat aatgataaac ttggaaagtg cttttgtggt
     6901 aagattgaaa agtttgatgg tacaggtgtt tatttttatt actttaaagt tgatgttgaa
     6961 atggatggtc agataaagac tttattttat ggaaaaaaca gagataatgg atattcttgt
     7021 gaatataatt atagtgatat taataaatat caaatcactg tttataaaga ttttaaagta
     7081 ccaacatggt acaaagaagg aatactttat catatatttg tagatagatt caataatgga
     7141 aatcgtagtg gtaaggttga tagtcctaaa aaaaatagtt tcatatatgg aaattgggaa
     7201 gatacaccta tgtacataaa ggatagtcaa ggggatgtta taagatggga tttccatggt
     7261 ggaaatttaa gagggataat caataaactt ggatatttaa aaaaattagg tgtaagtata
     7321 ctatatctaa gccctatatt tgaagcctct agtaatcata agtatgatac aggtgactat
     7381 aaaaaaattg acccaatgtt tggggatgaa gatacattta aagaacttat tgataaagca
     7441 aaagaaaaag gtatatcaat agtattagat ggagttttta gtcatactgg agcagatagt
     7501 aaatatttta atatgtatgg aaactataat agtttagggg catatcaatc taaagaatct
     7561 ccatattatt catggtatat gtttgaagaa tttcctcaaa aatataaaag ttggtgggat
     7621 gttaagacat tgccaaatat aaatgaatta gaacatagct atatggatta tataatatat
     7681 gacaatgata gcgttataaa taaatgggta aatatgggta ttaagggctg gagactagat
     7741 gttgcagacg aattaccaac taagtttata agagagttaa aaaaggaatt aaaaaaggca
     7801 gatgatgact caattcttat aggtgaagta tgggaagatg catcaaataa gattagctat
     7861 ggtcagagaa gaagttattt attaggtgaa gaattagatt ctgttatggg ttatcctttt
     7921 agaaataaca tgttttcttt cttaaaagga gaaattaatt catatgaact ttgtaataga
     7981 tatatgcaga ttaaagaaaa ttatccaaaa gaatcattta ggtctaattt aaatttaatt
     8041 ggaacacatg atgttacaag agctaaaacg gaattaaatg atgatgtaga tttagtgaag
     8101 cttgctgtag ctatacagat gacatttgaa ggagtaccat atatatatta tggagatgaa
     8161 gcaggtcttt gtggaggtgt tgacccagat aatagaagaa cttatccatg gaaaaatgaa
     8221 gacgaagata tgcttaattt ttatagagat gttataaaaa ttagaaataa gaataaactt
     8281 ttaagttctg gaaatacaga atttatatat actaataatg attctgtatt ttcttttatt
     8341 agagttaatg aaaataatga tagaatgtta attttagtaa atagaagtga aaatgtggaa
     8401 agtatatcat tatgtataga gagtagtttt attgaggaaa tacctataaa gtatagtctt
     8461 aaaaatgcaa atagtactat acaaattgaa aataatgagt tgaaagtaga tttagactct
     8521 aaatcattta gaatttttag agtaaattaa atatcttttt tt
//
LOCUS       DAKBNN010000162         8540 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000162 DAKBNN010000000
VERSION     DAKBNN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.163"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            98..2275
                     /locus_tag="OUB02_002980"
     CDS             98..2275
                     /locus_tag="OUB02_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCT8886716.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2289..2711
                     /locus_tag="OUB02_002981"
     CDS             2289..2711
                     /locus_tag="OUB02_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8886717.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3119..3922
                     /locus_tag="OUB02_002982"
     CDS             3119..3922
                     /locus_tag="OUB02_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCT8886718.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADAVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3915..4583
                     /locus_tag="OUB02_002983"
     CDS             3915..4583
                     /locus_tag="OUB02_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT8886719.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4819..5481
                     /locus_tag="OUB02_002984"
     CDS             4819..5481
                     /locus_tag="OUB02_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT8886720.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKLQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5541..6338
                     /locus_tag="OUB02_002985"
     CDS             5541..6338
                     /locus_tag="OUB02_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8886721.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6456..7646
                     /locus_tag="OUB02_002986"
     CDS             6456..7646
                     /locus_tag="OUB02_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCT8886722.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7755..8504
                     /locus_tag="OUB02_002987"
     CDS             7755..8504
                     /locus_tag="OUB02_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT8886723.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3392 a          855 c         1581 g         2712 t
ORIGIN      
        1 ataaatagat tattaataaa tataaaatct tattattaaa aatattctta gaaattacac
       61 tatataaaat ataaagtatg atatttgaat aaaaagaatg tataataagt ataataattt
      121 gtatttatta ttgggaaagg aaaatgttaa tatggatagt tatagacaac ttaaagctaa
      181 aataccctgt aaggaaacag gtatagaaat aaagaggact ttatgtgata tttgttctcc
      241 aggagtacat tgtggaattg atgcatatgt taaagatggt aaagtaataa aaatagaagg
      301 tacaaaagaa catccaatga ataaagggtt gttatgtacc aaaggtcttt gcaatagaga
      361 atatatctat agagaagata gaataaaaac acctctaaaa agaattggaa aaagaggtga
      421 aggaaaattt aaaccaatat catgggatga agcttataga gaaatagcag aaaaattaaa
      481 tagagtaaaa tctgagtatg gagcaaagca tgtagctttt tttacgggtt atggaaagtg
      541 gtatagacct tttttacaaa ggtttgcatc ttcatttggt tcacctaatt atggtacaga
      601 atctagttca tgttcaacat cagcacagat ggctggaaat gttgctactg gaagaaactt
      661 tacaatgcaa gatatggaga atgcaaaaac ttatttaggg tggggattta atccatacta
      721 ctctaaattt ttaaatagtg tgaatctaat aaaattaaaa gaaaaaggtc taaaatttat
      781 aattgtagac cctagaataa caccgacaac tcaaaaacta gcagatttac atttaagaat
      841 taaacctgga actgatggag ctttagctct tggaatggcg aatctattta tacaaaagaa
      901 tttaatagat agagaataca tagaaaagta tgtacatgga tttaatgaat ataaagaata
      961 tgtaaaatta tttgatttag atactgtaag tagaattaca ggagtttcta aagataatat
     1021 agtaaaagct gtcgagatgt tgtcacaagg tccattttgt ataaatgaaa gtgcagctcc
     1081 aatagttcat catcaaaatg gatttcaaaa ctatagagca atgatgtctt tatccgctat
     1141 aacaggtaat tatgatagaa taggtggaaa cataccaaaa caagattact ttgctggtaa
     1201 gataggtgga ttccaaacac tagaaaaaga gtttgtaaaa gaagttctac ctaaaaatat
     1261 aaatgaaaga attggtggag aaagatttcc tctatggaat gaaatggtga ctcaatttca
     1321 agctatggat ttacctaggc aaatagaaga aaagacacca tatgaaataa gagctttgtt
     1381 ttgtcatggg ataaatgcta gaatgtttcc taattcaggg agacttttta aagcgttgaa
     1441 tagtgtagat ttttttgtga atatagattt atttatgaca gatacatcaa aatatgcaga
     1501 tatactttta cctgcatgta catcatttga gagagaagaa tgtaagagtt atccaggagg
     1561 gtatattaca tataccaaaa aagttattga taaattatac gattcaaaat cagatgtaga
     1621 aatactttca gatttagcaa atgctatgaa tatagatgat gatttattaa aagcaggtta
     1681 tgaagcaagt gtaagacata tgtttaaaaa tacatgtgta gatgtagata aacttaaaga
     1741 aagtgattta ccattaaaat gtaaagattt taagccatat atagtaggtt catatacaag
     1801 agaagggtat gatactagca cttctaggtt tgaattaaaa tctacaatta ttgaaaaata
     1861 taaagatttt gggctggatg cactaccaac atatagagga tttaatgata atatagatga
     1921 tgaatatact catttattga catcaggagc aagaatttca aatgcactac attcaagact
     1981 tcatgacatc aaactttcaa gaatattaaa aaatgaagct agttgtgata tttctagctt
     2041 agatgcaaaa gaattgggtg ttatcagtgg agattatata aaaataacta ctaagatagg
     2101 aagtttggat gtaaaagtca atgttactga tagagtctta tataagaata tacatatgta
     2161 tcatggatat agagaagctg atataaatag tattatttgt gataattttg acccttatag
     2221 tggattccct gcgtatagaa gttgtaagtg taagattgaa aaaatgaata gataaatgga
     2281 gagatataat gaaaagttta tttaaattta ataaagaatt atgtacagca tgtggagcat
     2341 gtttgcttgc atgtatagac caaaatgata ataatataga caatggtgaa ctttttagac
     2401 atgtatttac aatagaggaa ggtgaaaata tagaatattt ttcattagca tgtatacatt
     2461 gtaatgagcc aaaatgttta ggtgtatgtc ctaaaaattg ttttaaaaaa gaagaaggat
     2521 tcgttgtatt agacaatcaa aactgtatag ggtgtaaact ttgtgaaaaa gcttgtgaat
     2581 atggagcctt aatttatgga aatgatggta aggcaaataa gtgtaatggt tgtattgaga
     2641 gatataagtg tggtatggat tcaccttgtg taatggtatg tcctacagga gcattaaaat
     2701 ttaataaata aattaaatat agagaatgac ttatacttag gtataagata tttttacaaa
     2761 aagctgtatt gagtttgtta taatgaaaac tcaatatagc ttttttatgg atatagttgg
     2821 atactataat gtgattctta gtaatagttt tttgctattg gacaaagact gtaaaacata
     2881 ttaaacttat gttgttcaaa gtcgaagtat aatataataa ctagaaaatt ggtttatagt
     2941 gctataaaag caaatttagt aaggtataaa atcagaggca atcctttact ataatatcaa
     3001 tatttgggag ttctgttatt gcagagctat taaacaatta taatggtaca agtgattcaa
     3061 cctttgaaaa tgtattatat aaaaaattaa aataagatta aaaaggagag aataaattat
     3121 gaaaaaactt aaattaatta ttatgtttgc cttggtatca gcactgttag ttacaggatg
     3181 ttcatcagga aataataaaa ataaagaaga acaaaaagtt ataagagttg caacatcagg
     3241 aacatattac ccttttgctt ttaaagataa agataaactt aaagggtttg aagtagattt
     3301 ttgggatgaa tttgctaaac gaactaattg taaagttgaa tgggtattaa ctgattttag
     3361 tggattattt ggattattag aagctgataa agcagatgct gtatctgctc aacttactgc
     3421 aacacctgaa agagagaaaa aatatgcttt ttcagatgct tataattata gtggaacaaa
     3481 cattattgtt agagaagatg atactagtat aaaatcaata gatgatttag ttggcaagaa
     3541 agtaggtgtg ggtactggag cagtagcaaa tgaaatatta aaagaaaaat acccaaatga
     3601 tgaaataaaa atagtaaatt attcaagtgc gactcttaaa gggaattatc aagacctaga
     3661 aatgggaaga ttagatgcag tagttgcgca ggatgttgag gctttaatag ctataaaaga
     3721 acaaaaatta aatcttaaaa tgatagaacc tccaattcaa tttggtgcct gttcttttgc
     3781 gcttcaaaaa aatgaaaaag gtgaaaaatg gacaaaagaa attaacgaag taattgaaga
     3841 gatgtataaa gatgggactt tgactaaaat atctgaaaac tggctaggaa aagacattac
     3901 taaggagcct attaatgagt aattttaatt ttgaattttg tctaagtata tttccatttt
     3961 tgattaaagc attgggaata acaatactaa tatctattgt tgcatttttt ttatcaatat
     4021 tattaggtgt aatacttgcc tttttcacac agacaaaatc aaaagtttta caatacataa
     4081 caagagtgta tgtttcattc tttagaggaa caccactttt aattcaaata tttttctttt
     4141 attttggatt tccaatgata ttttctttta tgaaagattg ttcaccttat ttagcaatag
     4201 taatttgttt aggtgtaaat tcatctgcat atatggctga aattatacga gcatctattg
     4261 agagtgttga taaaggacaa caggaagcat gttatgcttt tgggttaagt aaatatcagt
     4321 caatggttaa aataattcta cctcaagcag ctacatctgc agtacctcct attgtaagct
     4381 gtacactgga cataattaaa ggttcttctt taggtttaac tataggaata caagatttaa
     4441 tgggagtagc tcaagtagag gctgcttcaa gttttaaata ttttgagata tatatgataa
     4501 cgatattaat ttactgggca gtatctataa tattgagtaa tatacaaagc aaagttgaaa
     4561 aaagaataag aagagcatat tagatttcaa tatttaaaaa tgggatgata tagatatatt
     4621 attacttgta aataaatttt taattttaga aaaataaact tagtaatcta aattattgag
     4681 aaaatatagt atcaaaatgg actgtataat caagttaaaa aatagttttt ctattgaaaa
     4741 aataaataaa attctattgt aatttaatat gcgataaagt tttataaaaa acaacattaa
     4801 gctaggggga cttattttat ggcttttaat atgaaatatt ttttagaatt atttccaaca
     4861 ataatgaaat cgttcaatgt aactgtgatt ttggcaataa gttcattagt attttcatta
     4921 attataggta caatagtagc gttatcatct tattacaaag tgaaagtatt gaatcaaata
     4981 tgtaaagtat atgtatctat ttttagaggt acacctctta tgccacagtt attcttttta
     5041 tattttggac tagcatatat gagtgatttt gttaaaaata tggacccagt atttgcaaca
     5101 tctatagtac ttagtttaaa tatgggagct tatatgtctg aaacgataag ggcatctata
     5161 ctttcaattg atacaggtca gatagaagca gcatatgcaa tgggattaac aaatttacaa
     5221 acaatgaaaa ggatagtgat accacaggcc gtaagagttg cactgccatc attattcaat
     5281 aattttatag atttaattaa agggtcttct gtagcttttg ttgttggagt aaatgatata
     5341 atgggtgctg ccaaattaca aggagcgttg agttttaagt tttttgaagt gtatggagca
     5401 gttatggtaa tttactggtg tataataact cctttaggtt gggggcaaaa aaaattagaa
     5461 aagaaaataa gcaattatta agttattaag aaaatacata aaagtatttt tatataaaaa
     5521 atggtgtgag gagaagagat atgataaaaa gaaatattat aaaatgttta gtggcagttt
     5581 tatcgttaac gtttgtaatt actggttgtt caaaaaaaga ggaagtaaag aatgaaaaag
     5641 ttataactgt agcttcatct aattcagctg acccatattc ttatgttgat aatggtgagc
     5701 ataagggttt tgaagttgac atgtggaaag agattggtaa aagaagtgga tataagataa
     5761 aaatggaacc aacaggtttt agttctatat ttggtatgtt agattctggt aaagttgatg
     5821 tggcagccaa cttctttgga atgtctaaag aaagattaga aaaatacgat gcttctatac
     5881 cttatggttc tgattctgtg tgtatagctg taaaagatgg aaataacgaa attaataagc
     5941 ttgaagattt aaagggaaaa gttgtagcag tttcagaagg ttctcaaggt caacaggttg
     6001 caactagtgt taataaagat aatttgtttc aagaaaaggt ttacggtgat ggaactagtg
     6061 gtcttcaaga tttagatttg ggaagagtta gtgcatggat tgatgcagag cttactattg
     6121 tgggagacgc taaaaaagct aatatgaaag taagagtatt agataaaaaa ttatcagcta
     6181 ctaatatagc atacttcttt aagaaaaacg atgataaatc taaaacgata aaagaagatg
     6241 taaacaaagc aatagaagaa atgcttgcag atggaacagt gaaaaaaata tctgaaaaat
     6301 ggtttggaat ggatgtaagt gctgatatac aaaaataaat actttaaaat taaaatgtaa
     6361 aactgtgata actatagata agtatactaa atttatctat agttatctgt gtattacaat
     6421 gtatttagat tagtctttat aaatcaaaat gagttatgga ggtattgcaa atgattgaaa
     6481 ctaaaataaa atccattgca ctaaaaaata gagaaaaaat tataaaaata agacgacaga
     6541 tacacagtaa tccagagtta gcatttaaag aatataaaac atctaaactt ataaaagaag
     6601 agctaaataa acttaatata gaatacataa atattgctgg tacaggagta ttagctacta
     6661 taaaaggaaa aaataatggt ggaaaaacta tattactcag agcggatatg gatgctcttc
     6721 caatcaaaga ggaaaatgat ttagaattta aatcaataaa tgataatatg catgcttgtg
     6781 ggcatgatgc acatgtttca tggttgttag gaacagcaat gattttaaat gacataaaag
     6841 aagaattgaa tggaaatgta aaattactat ttcaacctgg cgaagaaaaa ggtggttctg
     6901 atattgtaat taaagaaaat gtacttgaag gtgtggatgc attagcgact ggtcattgtt
     6961 ggccaactat tgagtcagga aaaattggaa tcgctagaaa ttgtgctatg gcagcgacta
     7021 atacttttga aataacaata attggcaagg gtgggcatgg agcagagcct cataattgta
     7081 tagacccaat tgcagttgga aatgcagtat attcatccat tcaacagata gtttcaagaa
     7141 aaattgaccc agttgtacct gtagtagtgt ctgtttgttc ttttaattct ggtgtttcta
     7201 aaaatataat accagatgta tgtacacttc aagggactat tagagcgata agcaaggaac
     7261 aagtaattga agtaagtaaa attctagaat atatagtaaa aaatgtatgt aaagcacatg
     7321 gagcagattg caaatttgaa aagagtacag gaggagatgc tgttataaat gacaaaaaca
     7381 tgatagagat aggaagagaa agtgcttgca aaatacttgg agctgaaaat gttgaaataa
     7441 ttgattttcc tgcaatgaca ggtgaggatt ttgctattta tatgaaagaa aaacctggtt
     7501 tatttatgta tataggtgta ggagataaag aaaaaaatat aaattacaga ttgcatagta
     7561 acaagtttaa tatagatgaa aaatgtctta gtatagcacc ttcattattt tcacaactta
     7621 cagtagatta tttatgtcaa ctatagagta cattcataaa aatatattta gcctattttt
     7681 aaatttaatg ctagtatatt aattttgatg tagaacatat tatttattat atttatttaa
     7741 aaaggtggat aacaatgata aaagtagaaa atttacataa aagttttaaa gggacagaag
     7801 ttcttaaagg tataaattta gagataagtc aaagtgaagt agtagctata cttggatcat
     7861 ctggaacagg taaatctacg ttacttagat gtttaaatta tttagttgag cctacaaaag
     7921 gaaagataat gattggtgat gtaaaagtag atgtagaaaa agcaacaaaa aaagatataa
     7981 ccaatttaag aaaacataca tctatggtgt ttcaaaatta taacttattt aaaaataaaa
     8041 cagcattaga aaatgtaatg gaggcattga tagtagttca taaaaagagt aagaaagaag
     8101 ctagagaaat atctttaaaa ttaatagata aagtaggtat gttggatagg aaagattttt
     8161 atccatcaaa gttgtctggg ggacagcaac aaaggatagg tatagcgaga gcgatggcag
     8221 taaatccaaa tgtaatatta tttgatgagc ctacatctgc acttgaccca gaacttgttg
     8281 gagaagtttt aagcgttata aaatctcttg caaaagaagg tataacgatg cttattgtaa
     8341 ctcacgaaat aaattttgca aaagaagttg ctgataaaat tatatttatg gataatggtg
     8401 taatagctga agaaggaact cctgatgaaa tatttaataa tcctaaaaat gaaagaacag
     8461 caaaattttt aaatatcatt aggaaagaag aagtatttag ttaaaagtgt ataaattaag
     8521 cattagaaga tatgtaagat
//
LOCUS       DAKBNN010000163         8493 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000163 DAKBNN010000000
VERSION     DAKBNN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.164"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            57..377
                     /locus_tag="OUB02_002988"
     CDS             57..377
                     /locus_tag="OUB02_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HCT8886724.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            515..1159
                     /gene="trmB"
                     /locus_tag="OUB02_002989"
     CDS             515..1159
                     /gene="trmB"
                     /locus_tag="OUB02_002989"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCT8886725.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1288..2034
                     /locus_tag="OUB02_002990"
     CDS             1288..2034
                     /locus_tag="OUB02_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886726.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2163..2441)
                     /locus_tag="OUB02_002991"
     CDS             complement(2163..2441)
                     /locus_tag="OUB02_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886727.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2716..3162
                     /locus_tag="OUB02_002992"
     CDS             2716..3162
                     /locus_tag="OUB02_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886728.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3432..3884
                     /gene="accB"
                     /locus_tag="OUB02_002993"
     CDS             3432..3884
                     /gene="accB"
                     /locus_tag="OUB02_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCT8886729.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            3900..5261
                     /gene="accC"
                     /locus_tag="OUB02_002994"
     CDS             3900..5261
                     /gene="accC"
                     /locus_tag="OUB02_002994"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCT8886730.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5276..6130
                     /locus_tag="OUB02_002995"
     CDS             5276..6130
                     /locus_tag="OUB02_002995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCT8886731.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6151..7095
                     /locus_tag="OUB02_002996"
     CDS             6151..7095
                     /locus_tag="OUB02_002996"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCT8886732.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7284..7544
                     /locus_tag="OUB02_002997"
     CDS             7284..7544
                     /locus_tag="OUB02_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HCT8886733.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7697..8068
                     /locus_tag="OUB02_002998"
     CDS             7697..8068
                     /locus_tag="OUB02_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCT8886734.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8098..>8493
                     /locus_tag="OUB02_002999"
     CDS             8098..>8493
                     /locus_tag="OUB02_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886735.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKIL"
BASE COUNT         3310 a          900 c         1462 g         2821 t
ORIGIN      
        1 tgtatgggta tataatagat attgttttaa taaaatattt gaaagggaat gatttgatga
       61 aaattacttt accagaaaca gctattgata ctttaaaaag tatcttaaaa gacaatcaag
      121 ataaacctaa taatataaga gtatactttg caggtgttgg ttgtggaggt ccttcttttg
      181 gattagcttt agatgagaaa aaagaagatg acttaactta tgaagttgga gaattacaat
      241 ttgtaatgag ctctgatgaa tatagtcagt atggagatat aataatagaa gatacaggat
      301 ttggatttag agttattcct gaaaatatga aagatcaagg tggaggagga tgctctggtt
      361 gttcagggtg ccactaagat taagttttta gtaagtttag aactctcttt atagaaataa
      421 atttctattt taagagagtt ctttttattt tttggtgaga taatgtataa taaaaatgta
      481 ttttactaat tttttatatg taaggagaat tattgtgaga agaagacgaa aaaaaggtgc
      541 agatgaaaag cttttaagtt atacaaaata tgttttaaga gacgatatag acaaattaaa
      601 aggcaagtgg aatttaaagt ttagaaatga taatccaata catgttgaat ttgggactgg
      661 aaaaggtaag tttataacaa ctttagctaa gcagaaccct gacataaatt atattgccat
      721 ggaacttaag gaagaagttc ttttaaaggc tgttgaaaaa gcagatgctt ctaatttgaa
      781 taatatacta tttttatggg gagatgtaag caatattttg gattactttg aagctaaaga
      841 attatcgaga atttatataa atttctgtga cccatggcct aaaaatagat ggagtaaaag
      901 aagactcact cattctggat ttttagaaat gtacaatagg gttttggaag atgatggcga
      961 aattcacttt aaaacagata atgaaaaatt attcgagttt agtttaaatg aaattgctgc
     1021 aaataattgg ctattaaaga atatttctct tgatttagga aatagtgagt atgaaaataa
     1081 tgttacaaca gagtatgaag acaaatttat gtcacaaggt atgcgaatat ttagatgtga
     1141 agctaaaaaa cgtaactagt aatatgttta taagtttaat agtagaaatt tattttaatt
     1201 tatattaata aagtgtacga aagtaggtat atataacaag gaaacagaac cctctcatag
     1261 aaattaaatt attataataa aaaatttatg tgggagggta ctatgaattt agctaattta
     1321 ataccattta ttatttctgc agcagcttta agtccaataa attcaaatga taaggttttg
     1381 agcgaaaatc aacttaaaga tatttttcct gatgcaaatt taagagcagt tgtaaaaaga
     1441 tatataaacc cagatgagat gactataagc aatataaagg ctttagatgg agaattttat
     1501 gcaactggtg agagcatttc aaatttaaaa ggaatatcat atcttgaaaa tgtagacaat
     1561 tttatttttt ggaataataa tataaaagag gttcctaagg aagctttaag tctaaaagat
     1621 atggattcaa taaatcttgc caataattat ttgattgatg acgatgtagt aaattctctt
     1681 tctcataatg gtgtggatgt aaattgcgat ttaaatttta ttgatacaaa agataatcag
     1741 tataaactgg actctaaata tcataatgtt gatatagcaa aaggtgaaag tattgatttg
     1801 agaaaaataa ttacgaagaa aattgatagt tattataaat actgggaagt tactgataac
     1861 cttccaaaag atttaaattt tattgtatct gtaagtgata agagcgtact ttcatgtgaa
     1921 gatatgataa ttaaaggtaa taaaacaggt caagcagtgg taaaagttat actaagcgac
     1981 aaaaatgatt taaatacatc tcaagaagtt ctaattactg tcaatgtaaa ataatctgtc
     2041 aacttattca atcattctat tttcaattta agtgtataat caaaataaag gttgttagta
     2101 acagaacgct aatattagtg atttgttact agcaaccttt aaattttata gaaatagatt
     2161 atctaaaaaa ttccatttct tctgttagta gcccatattc ctattaaaat tatcagtgaa
     2221 tatattatat acaaaactat tcttatatat tggacatcta atccaaaaaa ttcaatgtta
     2281 ctctgtgagg tatgcaacat cacaaggact gcagatatac caataagcaa aggattgatt
     2341 actatttgct tatcagttct taaaatcatt attttaccca tccaaattat tgataatata
     2401 aaaaataaaa ttttaagacc aaattctaat gtatctacca aagcgaaaac atcccttcat
     2461 ttataataaa tttttcagct caacaaaata taaaacccta caaacaactt ttgaatccat
     2521 aataagataa cttgattaag cactatgaaa taagtactat aaaacttcat ctacattaat
     2581 atatactaca ctatacttca aatttcttaa acataaatta atgaaattaa caaaatttta
     2641 ttcagaaaca tataaagata tattttacta tttaaatgca tatcattata tatacagttt
     2701 taggaggtga ggtatatgga atctaaattt tttaattggt acactcaatc cttaggggga
     2761 acttttggca ttatatcatg tatatgcgca tatttaaatg gatacatgtt tgtttataca
     2821 aatagtgcta gcaaatattt tgaaagtata ggatttctgg gaataatttc aagttataca
     2881 ctacttccac tttgtatgct tactttgatt tttggagtta tatattctta tactccagaa
     2941 acatccgaca aaagtatttt taatatacct ttttttactt taaataaaag attaatactt
     3001 attacagtag tttttggttt tataggtgct agagtttact ttataattcc tgcaattcta
     3061 ataactttta acctatatag caatgttttg atagctaaat taaagtacat cctatgtaaa
     3121 aataaagaaa atgatactaa tttgaaagaa agcgaagtct aaattgcata atattggtat
     3181 ttgtcttaaa aattcccgag gtattaattt gtattctctt gtattttgca aaaaatattg
     3241 aattttattc attttatact tataatttat attaataatg gaaaattaaa acaaatataa
     3301 gttactggtt gttaatttaa aattaaaaga tatattgatg tgttgattct ctagtcagca
     3361 ctttttcttt tataaatttg ttttttgtga tataatatta gcaccaggta ataatacaag
     3421 ggggtaacaa aatgaacatc aatgaaatta aggaattatt aaaggccata gactcaacta
     3481 acttagaata tgtaaaactt gaaagtagcg atttaagatt agaagtatct aaaaaagctc
     3541 aatctacaag ttcacctgtt ttaagtgtac aacaagaatc agtagtggat ctttctttag
     3601 aaaagccagt tgtaaacgat acacctgtca catctaatga gaatctatca gtagtagtag
     3661 ctcctttgat gggaacattc tatgattctc caagtccaga tgcagatagc tttgttaaag
     3721 ttggcgatgt agttgaagaa ggcgacactt tatgtatatt agaagcaatg aagcttatga
     3781 atgagataac aagtgaaatt aaaggtgaaa taattgaagt attagttagt aatgaagaac
     3841 tagtagaata taatcaacct ttatttaaga taaaaccatt gtagtaggag gtatttaagt
     3901 tgataaaaaa aatactagtt gcaaatagag gcgatatagc agttagaatc atcagaacat
     3961 gtaaagaact aggtataaaa actgttgcta tctattctga aatagataaa gattgttttc
     4021 atagatatat agctgatgaa tctatttgta taggaccaaa taatataagt aagagttata
     4081 ataatataga aaatataatc tatcttgctc ttaaattaaa gtgtgatgct attcatccag
     4141 gctttggatt tttatcagag aatcctgaat ttgcaaagca atgtgaagat aataatatta
     4201 tttttatagg tccaacaaga gaacaaatga ttttgatggg agataaatct agagcaagag
     4261 aaactatgat ggaattgaat atccctgttg ttcctggttc agaatctgtt ttgaaaacta
     4321 aggaagaagc tttagaagtt gcaagagaaa ttggatatcc tgttatgata aaagcctcaa
     4381 gtggaggcgg tggaaaaggt atgagaatcg ttagaaaaga agaagaactt ttttcaaatt
     4441 ttgatatggc aagttcagaa gctctagcag cattttctaa tagtgattta tatatggaaa
     4501 agtttataga aaatcctaga catatagaag tacaagtatt tggagataaa cattcaaatg
     4561 caatacattt aggtgataga gattgttcta tgcaaaggcg aaatcaaaaa gtaatagaag
     4621 aatctttaag tccatacctt tcagatgaag aaagacaaaa acttcataaa attgcagtag
     4681 atatagtaaa aggagtaggt tatatcggtg caggtactat tgagtttata gttgacaaag
     4741 ataaaaactt ttattttata gagatgaata caagaataca agttgaacat ccagttacag
     4801 agatggtaac aaatcttgac cttataaaac ttcaaatatc tatagctaat ggggataaaa
     4861 ttccttttaa acaagaagat attactttta gaggtcatgc tattgaatgt agaataaatg
     4921 cagaagattc atcaaagaat tttgctccat caccaggtaa gatagaatca ttaaatttac
     4981 ctggaggttt tggtgttaga tttgatactt ttgtatatgc aggatatact ataccacctt
     5041 tatatgattc tatgataggt aagttaatat gttgggctga aactagagaa gaatgtataa
     5101 gtagaatata tagagctcta gacgaaatta ttgtagaagg tattaataca aatgtagaat
     5161 ttcaaaaggc acttgtaaca agtgaggagt ttagagaaga tactcatcat accaaattta
     5221 tagaagatgt ctttatgaag aaagaatttg ctacattata aggagtaaat agattatgat
     5281 aaaaaaattt ttatcatcaa aagaatctaa gtatgttacg atttctttag atgacaactt
     5341 taaaaagaat agtgtagatg ataaattttg gacctattgc aaaggttgtg atagtcatgt
     5401 atttagaaag gacatagaag aaaattcatt tgtatgtcca aaatgttcaa gacattatgg
     5461 cttaagagca agaaaaagaa taaatttatt gattgataaa ggaactttta tggagtttaa
     5521 ttctgatgta gaatttcaaa atccactaaa ttttcctaaa tacaaagaaa aagtagactc
     5581 ttataaggaa aaaacaaaag agtcagaagc tgttgtcaca ggttatggaa gaataaatgg
     5641 aattaaaaca gttatttgtg taatgaatcc tgattttatg atgggaagta tgggttctat
     5701 tgttggggaa aaaataactt attcaataga gtatgctgct gaaaataatt tacctataat
     5761 tatttgttct gcttcaggag gagctagaat gcaagaaggt atggtatctc ttatgcagat
     5821 ggcaaagact tctcaagcac tttctaagct tgaagaaaaa tcattgccat atatatcagt
     5881 tctaactgac cctactactg gaggagtaac tgctagtttt gctatgcttg gtgatattat
     5941 aatttctgag ccaaatacgt tgataggttt tgcaggtcca agagtaatag agcaaacaat
     6001 aaatcaaaaa cttcctgaag gatttcaaac ttctgagttt ttacttgaaa aaggttttat
     6061 tgacatgata gtagatagaa gaaaaatgaa ggaagtatta tatcaaattc ttgctatgca
     6121 caagaaatag ggcataaagg aggaactgtt ttgattgaaa ataatagtga aaaaataaaa
     6181 gtcttagaaa atgatattaa acaattaata actatttcta aggcaaataa tatagattta
     6241 agtgataaaa taaattcact taatgagaaa ttggaaaagc ttaaggaaga tgctttttca
     6301 catctatcag catatgaaaa ggtaacacta agtagagata taaagagacc tacgacattg
     6361 gaatacatag aacatatttg ttctaatttt ttagaacttc atggagatag gctttttaaa
     6421 gatgaccctt ctatggttgg aggaattggt cagataggaa agtttaatgt tactatagtt
     6481 ggacaccaaa aaggaagaga tacaaaagaa aatataaaaa gaaattttgg aatgcctcat
     6541 ccagaaggat acagaaaagc attaagactt atgaaacaag ctgaaaaatt taatagacct
     6601 ataataacgt ttattgatac atcaggagct ttttgtggtc tagaagcaga agaaagagga
     6661 caaggagaag ctattgcaag aaacttactt gaaatgagta aactttctgt tccagtaatc
     6721 acttttgtaa ttggtgaagg tggtagtgga ggagctttag gtatcggagt aggaaatgat
     6781 gtatgtatgt tagaacattc agtttattct gtgatttctc cagaagggct ttctagtatt
     6841 ctatttaaag attcatctaa agctaaagaa gcttgtgatg ttatgaagct tacaagtaat
     6901 gatttatatg atttaaaaat aatagataag attataaaag aacctcttgg aggagcacaa
     6961 aaagatgtag aagctgtttc aaaagaaata aaagcttaca ttttagaaag attaaatcat
     7021 tacaaagata tggataaaga ggaaattata gctcaaagat ataaaaaatt tagaaatatt
     7081 ggaaaatgct tatagaatat aaagaaattt gtagtttttt cataataaaa tggttaaaaa
     7141 aaataaaggt tttcttaaaa cgattataga agtatatttt tataaaagta tactttaaat
     7201 gatgattgta attagtaaat ggcaattatg atttagtact ttaggtaatg gatactttaa
     7261 caaattaaca aggagatgtt gaaatggagg tactaaaagt ttcatcaaaa tctaatccta
     7321 attctgtagc tggagccttg gctggagtac taagagaacg aggcgttgca gaaatacaag
     7381 ctataggagc tggagctcta aatcaagcga taaaatcaat tgcaatagca agaggttttg
     7441 ttgcacctag tggaatggat ttagtttgca ttcccgcatt tactgatata gaaatcgaag
     7501 gtgataagaa aacagccatt aaacttataa ttgaacctag ataatatatc tctttattag
     7561 aatttaatta agttttaaag gttggctata gaaataggac actatagtga acctttttta
     7621 ctttatatgt tttattttat gtaactttta atgtataatc ttaagtaaac aaataagatt
     7681 ggaggaaata tacattatga aattaaaatt attggtagaa gagtatgcag tatgtagatt
     7741 aaataatgac agtaaaatac caacatggat agatacagag aagttttact ctataactag
     7801 aactgatgat gaattaagtg ttgtatgttt aaataataat attccttctg atgtcaagtc
     7861 agaaaaggag tggagaatac ttaagatact aggtccttta gacttttcac ttgtaggaat
     7921 tttatctaag ttaagtagtt tgttagcaga caacaaaata agcatatttg caatttctac
     7981 gtatgataca gactatatct tagtaaaaga aaaggatata gaaaatgctt gtaaaatttt
     8041 aagctgtaat ggatatgaag tagagtagtt aaatactttt acatgaggac ttaacttatg
     8101 atttatataa tcgaaatttt aaaaataagt gtaataaata ttttttgttt cataggaata
     8161 tttattgtat ttggattgat ttttagcatt acagaaaatc taaataataa atttatcttt
     8221 tcatcttttg gaaaaactgg aattattgta actggagcta taggaacatt tgttcatgaa
     8281 ttgagtcatt tgataatgtg tttaattttc atgcataaaa taaattcagt taagtttttc
     8341 agaccaatag aaagtaaaaa tgatggtata ctaggatatg taagtcatag ctataaaaaa
     8401 gataatcttt atcaaagtat aggaaatttc tttataggaa ttgcacctat tataggtgga
     8461 acaataatga ttattttaat ttttaagata tta
//
LOCUS       DAKBNN010000164         8452 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000164 DAKBNN010000000
VERSION     DAKBNN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.165"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            35..790
                     /locus_tag="OUB02_003000"
     CDS             35..790
                     /locus_tag="OUB02_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8886736.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            921..1943
                     /locus_tag="OUB02_003001"
     CDS             921..1943
                     /locus_tag="OUB02_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8886737.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2107..2664)
                     /locus_tag="OUB02_003002"
     CDS             complement(2107..2664)
                     /locus_tag="OUB02_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCT8886738.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2670..3746)
                     /locus_tag="OUB02_003003"
     CDS             complement(2670..3746)
                     /locus_tag="OUB02_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCT8886739.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLNFPQEYLFCFRYNYNKKE"
     gene            complement(3747..4430)
                     /gene="modB"
                     /locus_tag="OUB02_003004"
     CDS             complement(3747..4430)
                     /gene="modB"
                     /locus_tag="OUB02_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCT8886740.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4579..5391)
                     /gene="modA"
                     /locus_tag="OUB02_003005"
     CDS             complement(4579..5391)
                     /gene="modA"
                     /locus_tag="OUB02_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8886741.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYQVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5584..6513
                     /locus_tag="OUB02_003006"
     CDS             5584..6513
                     /locus_tag="OUB02_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886742.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6674..7123)
                     /locus_tag="OUB02_003007"
     CDS             complement(6674..7123)
                     /locus_tag="OUB02_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886743.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7326..8306)
                     /locus_tag="OUB02_003008"
     CDS             complement(7326..8306)
                     /locus_tag="OUB02_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8886744.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2941 a         1380 c         1009 g         3122 t
ORIGIN      
        1 accaaaaata aatcagaaaa aagaggtgtt gtagatgtta caaattaaaa atctttcaaa
       61 gagtttccct aatccatatg gtgaacctaa tactatcttt gaaaatttat caatcgatat
      121 agaagatggt gaatttgtaa gtatcatagg tagtaatgga actggtaaat ctactttatt
      181 aaatataata tccgggctta taaaagaatc ttctggtcaa gtattattaa ataaacataa
      241 cttgtctaat ttagctgaac acagaagaac tcaaatagta agtagagttt ttcaagaccc
      301 aagtcttgga acttgccctt ctatgactgt tagagaaaac ttgtctttag ctcttaacaa
      361 aggaaaacta ttaaatctaa gaaaatgtct tcgttacaaa agagatttct tagaaaattt
      421 attggaaggt gtatctcttg atttaaaaaa ataccttgat gttcaagtaa aatttttatc
      481 aggaggtcaa agacaatcat tatcactaat tatgtcgtgc ttaacaaatc catcagtttt
      541 attacttgat gaacatacag cagcacttga cccaaaaaca tcaaatgaag taatagagtt
      601 aacagataaa atcgtaagag aaaaaaatat aactacactt atggttactc acaacttaaa
      661 acacgctctt cattatggtg atagattgat aatgcttcat aagggtgaag ttgtattaga
      721 tgtaaaaggt aaagaaaaag aacaattaac agttgaagaa atcttagaaa aatttgaata
      781 tgcagtttaa aataatatat ctattgtatt aaactcggct ttaaaataaa gttaaaatta
      841 caataattat ggttactttg taataattca tatgaatata tgaacatata taataataaa
      901 tttttgaggg ggatataaaa atgattaata aaaaaagatt ggcaagtttg attttagctg
      961 gtgctcttag tatatctatg cttacagggt gttcacaagg aggagactct ggtaattcaa
     1021 aacaagaaag taattctaaa ggtaaagaag ttaaaaaaat tggtataact caactggttg
     1081 aacatccagc attagatgcc actagaacag gttttgtaaa agcattagaa aagaacggat
     1141 ttaaagatgg agaaaatatt gatatagact tccaaaatgc tcaaaatgac atgcctacta
     1201 cacaaagtat tgcgagtaaa tttgcatctg ataagaagga cttaatattt gctatatcta
     1261 ctccatcagc tcaggcagca tttaatgcta ctaaagatat acctatactt attactgctg
     1321 tgagtgaccc tgttgctgct ggattagtta aaactcttga aaagccaggc acaaatgtat
     1381 ctggaacatc agattttgtt tctgtagaca aaggtttaga acttcttaaa atatttgctc
     1441 ctaaggcaaa aactatagga gttatgtaca atactagtga agtaaattcc aaagtccaag
     1501 ttgatgcttt aaaagaatat gcatctaaaa atggatttaa agttgttgaa aaaggtataa
     1561 ctacttcaaa tgaagttaac caaggtattt ctagtttagt tggcaaaata gacgtattat
     1621 atgttcctac agataactta gtggcttctt ctatgcctat agtctctaaa atagctactg
     1681 agaataaaat acctgttata gcagctgaat ctgggccagt tgaaaaaggt gctcttgctt
     1741 gtcaaggtat caactacgaa aaactaggtt acaaaacagg tgaaatggca gttaaaattt
     1801 taaatggtga atcagtatct gatatgcctg tggctacatc agatgataca gatatcattg
     1861 ttaatgaaga tatattaaaa gctttaggta tggaaaaacc ttctaatgaa aatatttctt
     1921 atgtaaagac taaacaagaa tagaaagata ttttctatat acgattaata tgcctgtttc
     1981 tataaattaa tttatagaaa caggcatata tttttatttt aatgtattat ctatttaaag
     2041 attatattta aatgctctta atttgtttgt ataaatttta cttgtataaa ctttatacaa
     2101 tcatatttat tcttctattt tctttattac tctacatgga tttccaacag ctacagtatt
     2161 tggtggtata tcttttgtga ctacacttcc tgcacctatt actacattat cacctatagt
     2221 aattcctggt gttatgatta ctcccccacc tatccataca ttatcaccta tttttacagg
     2281 agaaccatac tctatgcctg aatttctaag ttgtgcatca attggatgat aggctgtata
     2341 gatttgaacg tttggagcta acattacatt gtcacctatt tcaattttac atacatctaa
     2401 aaaaatacaa tcataatttg caaagaaatt ttcgcctaca tgtatgttat atccataatc
     2461 acacctaatg ttttgttcta catttatttg cttgccaact gaaccaaata gttgtcttaa
     2521 aatatcttct cttttttcat attcatcctc taaagtatta ttaaacaatc tagttaattt
     2581 tttacaatac tctctttctt ttactaacaa ttcatcattt gcatagtatc ctttcccaga
     2641 caacattttt tctttttcag tcatatagtt tactcctttt tgttataatt gtatctaaag
     2701 caaaataaat attcctgtgg aaaatttaaa tatattctgt cacctttctt aaatctcatt
     2761 ttactttttt ccaattctat ttgaataggt acacattcgt tagataaatc agtttttcta
     2821 acatatatag tatatgtaaa aggattttct acaatgttaa tcaattcgaa acataacttt
     2881 tcatttaaat cttcattaga atccaagacc ttaatatgat gctccctgat tcccacatat
     2941 tctatattat cacctatttc tctatttaat gtcaactcgc atccccagtc tgtagcataa
     3001 atagtatttt tatccagtct atttaatttg gatatatttt tacaccctgt tatcttagct
     3061 tctataaggt actttggaga ttcaaagagc tcttttttag gtctctttgg aagaccttct
     3121 cctttgttgt acactataat atcatcacaa actcggtaag cttcttcaat atcatgagta
     3181 acaaatagta tatctccctt gtaatctttt aacatattca taagttcttt ttccatatta
     3241 cttcttaaat gatggtccaa tgctgagaaa ggttcatcta aaagtaatat atctggagat
     3301 gttgccaatg ctctagctag tgctaccctt tgttgttgac caccagataa ttgccaaggg
     3361 tatctcccct caaacccatt taatttaagt atatctaagt accttgctac tatttccttt
     3421 ttttcactct tacttagttt taaaagccca agttctatat tttgacttac agtcatatgt
     3481 ggaaacaaag catagttttg aaataaaaat cctacctttc tatcttgtgt tgataaattt
     3541 atcttctttt cagaatcaaa tagaactcta tcatttaata caattttacc tcttgttggt
     3601 ttctcaagcc ctgctataca ttttaatgtc atactttttc cagaaccaga ttctcccaaa
     3661 aaccccagag tacctttttc ttgtttaatt tctaccttta gtttaaaaga ggataaatct
     3721 ttttcaatat ccacgtataa gctcaactat ccacacctct ttcctataat tttctgctga
     3781 aattccgacc aataatttag taataaaatc attgctatag atattgctac aataatcata
     3841 acccacatca tagctttatt catatctccg ccttcaacag caaaaaatat agctattggc
     3901 atagtttgag tttttccagg tatatttcca gctatcataa gtgtagcacc aaattctcca
     3961 agagctctgg caaacgacag caccaatcca ccaattattc caggccatgc taatggaact
     4021 gcaatcttaa aaaatacttt ccattcatta agacctaatg ttctagcagc tgacaacata
     4081 ttattatcaa tctgttcaaa agctgacctt gaagttctat acatcatagg aaaagacacc
     4141 actactgctg aaattacagt tgctgtccaa ctaaatatta aagtcctatc gaaatttaat
     4201 aaaaactttc ctataaatcc attcttccca catagtaata ataaaaagaa acctacaact
     4261 gttggtggca aaattagtgg aagtgtaaat agtccatcta taagaccctt ccacttacct
     4321 ctatagttag ccataatata tgacacaaat attcctatta caaaagttat tattgttgac
     4381 aaaatagatg tcttaagtga tatccataag ggtgaccaat ctgttcccat ctttccctcc
     4441 ttaattttaa atggaaatag tgaaactaat aactctatta atttcactat ttccatttta
     4501 ttataataca caattatttt caattttcat ttatttatat ataatttata cttaattaca
     4561 cataatttat ttctaagttt attctacttt tttatatcca aattcttcaa aaatcttttg
     4621 tcctgactca cttaataaaa attcttcaaa tttcttagca gcatctacgt ttttactagc
     4681 tttaattact gaaacttgat atgtaatagg cgaatgagtt ttttcgtctg ttttttcaac
     4741 tacttttatc ttatcattat tgacagtatc actgaagtaa acgaatccaa catcagcatt
     4801 tcctgattgc acccatgcta aaacttcttt tacatctttt gcaaatacta gcttatcttt
     4861 taacttatct tttaaattta aattagttaa cacctcatct gcatacttac cagctggaac
     4921 actttcagct tcaccaacag caattttctt cactttatct gtagtcaaat ctttcatacc
     4981 tgatacacta ctgtcagcag aagaaataag aactaaatca ttttttacta aatctttcat
     5041 agtatcagaa actaataact tctcttcatc caaagctttc atttgttttt gacctgctga
     5101 aataaataaa tcacaaggtg ctccttgttc tatttgttgt tgtaatgagc ctgatgctcc
     5161 atagttaact gtaagtttta catttgaatc aactttttta tattcttctt caatctttgc
     5221 cattgcttct tttaaacttg cagcagccga tatatttaac tctactgaat cactactttt
     5281 agttgttttt tcttgttttc catcatcttt tttgtcttca ttactattgc acccaactgt
     5341 ccccaaagtt aaacatgcaa ctaatcctaa tattcccaat atttttttca taacaatctc
     5401 cctttttgta aatttgatac acattttaac atgttttatt atgttttatc atgttttgtt
     5461 atatatagtt taattatata tacatgataa taaattttca agtaatttat ttacactact
     5521 tttaaatatt ttgctacatt ttcctttttt taatgttaaa ataaccttat atggaggtgg
     5581 attatggact cattatcatc tttgacacct gtcgaggtag ctgaattatt aaagataaca
     5641 aaaaatactg tatatgaatt aatcaaaaga ggtgaacttc cttcttataa agtagggaaa
     5701 aaaataagag ttgatactaa agatgttgaa gaatatatca acagccaaaa aactggtaaa
     5761 gtaaaggata cacatttcaa caataaaaat atagatataa ataataattt caaaattatt
     5821 atttctggtc aagatgtaat attagacatt ttagctaggt ctgttgaaaa aaaattggat
     5881 ggaataagta catttcgctc ttatataggt agttataatg ctttgtatga gttatataat
     5941 ggcagagttt ctatagcatc atcccatcta tgggatttcg aaacggatga atataatagc
     6001 acttttgtta aaaagctatt gccaggaata ccatgtgtac ttataaatct agcttataga
     6061 atgcaaggtt tctatgttgc taaaggaaat cctaaagaga taacaacctg ggaggattta
     6121 acaaaaccaa gtataaccat tgtaaatagg gaaaaaggtt ctggaactag ggttttacta
     6181 gatggtaaac ttagacttct aaactttaat ggtaaacata taaaaggtta tgataatgaa
     6241 gaattatcac atcttggagt tgcaagtact gtttctcgtg gaatagcaga tgttggtttg
     6301 ggaaatgaaa aagctgcttt acaagtaaat aatatcgact ttattcctct tcacaaagag
     6361 cgctatgatt tagttattaa aaaagaagac ttacaaaatc cagtttacca aactataatt
     6421 aacataataa actctcctga gtttaaagct gaacttcaag gtcttggcgg atatgattta
     6481 actgatactg gaaaaactat agcaaaagtt taagattaaa atcgcaatct attttataag
     6541 atttataatt tttcacaaaa tttataactt aaacacgaaa taaattgcga ttttagtgtc
     6601 tcaaaaaatt tatgtctttc attttatcat ccaatacaaa atataatata attaattgtg
     6661 caatcatatt atattaatat atatttacat aatagtattt ttctttaatg tagattaaca
     6721 tatgatatgt tttaaacatc aatccccttg ctttcttata tatatgtggc aacacatatg
     6781 agtttaataa attatataca atgttgcttt cttcttgtat tttttcaacc aacaagttat
     6841 ttataaaatt acctttctca ttttcaataa tctcactcca atttatctta acttttaata
     6901 tatatcttcc cactaggtat acacactcta caataaataa taaaaataca atgcagtcag
     6961 tacctgttgt cttatataaa ttacccgata ctaatggctt tgcgaagaaa ttataaaaat
     7021 tcaaagtatc acccgtataa tcaaaaagta ttaatagcac tcctagaact ctcaatactt
     7081 taatattcaa catgttttta tctttattat ttttgttttt catatatact acctctcttt
     7141 tctttagatt tgattgtaat ctaatactaa ctcttttatt tgaggtataa atgaatttaa
     7201 ttggtagtta ttttcatttt tactagaaat taattcacat tactaaacaa aacttatatt
     7261 taaacataaa aatagcctat ctatctttta ataaatacaa aaataaataa gctactaata
     7321 ttcaattaac ttttatcact taaaacatta ctatctataa atttaaatat tttattgaag
     7381 tatactgtac tatcatgttc aaatccatca acatgaccaa taccttctat aagccatagg
     7441 tcttttttat catgtttaac taaatcatac attttcttgc tattttgata attacaaaca
     7501 gtatcttttg ttccatgtat aaacagtatg ggtacattag atttctcaac tgactcatat
     7561 ggttttacat cactgtacca aaatccagat ttaaacttat tatataaatc tccacaagtc
     7621 accacataat tgattaatat atctggtatt cgtttttcta gtactcccat tctaattgca
     7681 tctttcattt cagaataagg agaatccagt atataaaaat cgaccttatc atcttttgaa
     7741 tttatctctg aatgcatagc agctgtccct gcccccatgg aaaatccatg cactcctaat
     7801 cttccttcag gaaatttatt ttttacaaat ttaactagac tatctaaatc atacctttca
     7861 taaagaccaa atgtgtaatt atctccacca ctattaccag tatttctttg attataaact
     7921 aatacattat aacctttatc taaatatcta tatgctacct ttaacatttc atagtaacta
     7981 cttccaattc catgaacaag tatcatagta tctcttgtct ttatatttga agttataaaa
     8041 agactttcaa gcttgtaatt attagaactt tgtatcatta attcagatgt cttgtagttt
     8101 tttaatttct ccagtggttt atcttttctc tcagaaaata tttttactat atcttcgttt
     8161 tttattaact gctctaaacc aactgaacta ttatacacta atttgccaaa tatataagat
     8221 acaattaata ggattaatag aaacacaatt gatataatta taaatttatt tcttaaacta
     8281 atacaatttt taaaactatt tttcatattt atttaccata caattttcct atattatatt
     8341 ttctaagtaa tagcataatt gattatacta ttcttataat ttctattata cctacttatt
     8401 tattaaaaat acaaattata ttttattaat ttttactact tgtaaattat ta
//
LOCUS       DAKBNN010000165         8387 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000165 DAKBNN010000000
VERSION     DAKBNN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.166"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(210..1886)
                     /gene="bclA2"
                     /locus_tag="OUB02_003009"
     CDS             complement(210..1886)
                     /gene="bclA2"
                     /locus_tag="OUB02_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HCT8886745.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGVNGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2244..2783)
                     /gene="hpt"
                     /locus_tag="OUB02_003010"
     CDS             complement(2244..2783)
                     /gene="hpt"
                     /locus_tag="OUB02_003010"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCT8886746.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3111..4418)
                     /locus_tag="OUB02_003011"
     CDS             complement(3111..4418)
                     /locus_tag="OUB02_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCT8886747.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDVAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4843..6618)
                     /locus_tag="OUB02_003012"
     CDS             complement(4843..6618)
                     /locus_tag="OUB02_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886748.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKVDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6615..6752)
                     /locus_tag="OUB02_003013"
     CDS             complement(6615..6752)
                     /locus_tag="OUB02_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886749.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6830..7399)
                     /locus_tag="OUB02_003014"
     CDS             complement(6830..7399)
                     /locus_tag="OUB02_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT8886750.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7583..8218)
                     /locus_tag="OUB02_003015"
     CDS             complement(7583..8218)
                     /locus_tag="OUB02_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCT8886751.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2393 a         1664 c          964 g         3366 t
ORIGIN      
        1 tttattggta tatgaaatca tattgtttat tattttatag tcttttcatc atataaacct
       61 attaaatatt atcctattta ttggtatatg aaatcatatt gtttattatt ttatagtctt
      121 ttcatcatat aaacctatta aatattattc tatttattgg tatgtggaat tatattgttt
      181 attattttat agtcttttca tcatataaac tattgtattc tataaactga tacatatcca
      241 gctgcagaag tacctgtcgt gcctgaacca aatgtaaaac ttaaagtaac agctgctgct
      301 ctagcatcaa ctaaaaagct taaacttaca cttacatctg atgaagtaag tgtatttact
      361 gcttttgcaa taaagttatt ttgagctact ccattaaagt tagtagtaat tgacataaat
      421 cctgcaagtg aaagtgtggc acgtataccg aaagatacat tgtatatccc tccaactgca
      481 agatttatag tactacttgt tggaaaagtt atattagttc catttataaa tgatgaatta
      541 aatgtcacta atgtattatt cactaaactt gaagaggaca ctgtaaattg tgcattgttt
      601 gctgctaaca ctcctgttgc tcctgtcgcc cctgttggac ctgttgctcc atttgctccg
      661 gttgctccgg tcgcccctgt tgctcctgtc gctccatttg ctccggttgg gcctgttggt
      721 cctatcgctc ctgctactcc atttgctccc gttgctccta ctgctccggt tgggcctgtt
      781 gctccatttg cacctgttgc tcctgtcgct cctgttgctc ctgctactcc atttgctccg
      841 gttggaccgg ttggacctat tggacctgct actccattcg ctccggttgc tcctactgct
      901 cccgttgggc ctgttgctcc atttacacct gttgctcctg tcgctcctgt tggacctact
      961 gctccatctg ctcccgttgg gcctgttgct cctactgctc cggttggacc tattgcccca
     1021 gctactcctg ttgctcctgt cgctcctgtt ggacctacca aaccatttgc tccggttgct
     1081 cccgttgggc ctgttgggcc taccaaacca tttgctccgg ttgctcccgt tgggcctgtt
     1141 gggcctgttg gacctaccaa gccatctgga cctgttgctc ctactgctcc tgttggacct
     1201 acctctccat ctgctcctgt tggacctgtt gctcctattg ggcctgttgc cccagttgtt
     1261 cctgttgctc cagtcgctcc tatacttcct gttggacctg ttgctcctgt atttcctgtt
     1321 gggcctgtag cacctgttac tccatttgct ccggttgctc ctgtatttcc tgttggacct
     1381 gttattccat ttgctccggt tgctccggtt gctccagtta gtccggttgg acctgttgct
     1441 cctgttgctc ctgtattccc tgttggtcct gttattccat ttgctccagt cgctcctgta
     1501 tttcctgttg gtcctgtaga gcctgttatt ccatttgctc cggttgctcc tttgtttcct
     1561 gttggtcctg ttattccatt tgctccggtt gctcctgtat tccctgtcgg acctgttatt
     1621 ccattcgctc cagtcgctcc tgtatttcct gttggtcctg tagaacctgt tgttccattt
     1681 ggtccagtcg ctcccatatt tcctgttgga cccgttattc cattcgctcc agttgctccc
     1741 gtatttcctg ttggtcctgt tattccattt gctccggtcg ctccggttgc acctctaggc
     1801 cccgtcggtc ctgttggacc agtagcacct gttggccctg ttggacctac atcttgatat
     1861 aaacttggac ctgaaatatc actcatataa ttaatcctcc ttttttaaag ttagagtaat
     1921 actctataaa tattgagtat taccctatat aattatatga atcgacaatt tcttatgtga
     1981 taatgtatct atttgcttat atcaacatat atgctttgat tcttgaattt cattactttt
     2041 ttgtaaaatc ttttacaatt attaatacaa ttaaaaaact catttcccat caattattta
     2101 aaattttttt attatgttat aaaaaataga taattaaatt gcataaaatt aatttaatac
     2161 tactttaatt actttttaaa taaaaaaacc tcaacacgat taataaaaat catattaagg
     2221 catccacaaa attatataat tgcttatcta tcttcatcag taacattgaa tatataagga
     2281 acatttctat aataatcacc aaaatcaaac ccatatccaa ccacaaactt atcttctatt
     2341 tcaaatccac aataatctgg cactaaatcg acttttcttc tgcttggttt atccaaaaga
     2401 acacagcatt ttaaactagc aggattttta gcttttaaat gttttaacac aaaatccata
     2461 gtaagtgcag aatcagtaat atcgtctaca accaaaacat catatcctgc tatatcagta
     2521 gtcacatctg aaactacttt aacctttcca gtactttctt cattgtttcc ataactagat
     2581 gtagtcataa aattaactct aagtggtaag tttatatttc taactaagtc tgcacaaaat
     2641 ataaagctac cttttaatag agaaactacc ataagatttt tatctttata atctttttct
     2701 ataattgctc ctaattccct taatttatct ttaatttttt cttctgaata caatacttcc
     2761 catttttttg tttctatatc cataaaaaga cctccaattg taataatcct tcttaatttt
     2821 ataagagatt gctcactttt tcaatactct tattcaatat tagtcaatat tattgagttt
     2881 ttatgttcat tcaacaatta ttaacaatgt atcttatatt ttaagtagtt catatatcca
     2941 cactaaatta catactatat attttagaca aaaaaagaaa aaacttgcac taacaagttt
     3001 tttctattat acataaattt atacataatc atattttatt agagggtact aattaaattt
     3061 atttatgcta tttttgttat ccctcataat acaaaacata ttataaagta ctagtcaact
     3121 ttattcatat cattcattac atttaagatt atctcatctg ttatagacat acctttgtca
     3181 cttactctac ctaagttttg tatactttgc tcaacacttg aaccaactat accatttagt
     3241 ggtggtacaa aacaatcttg cttagctatt atagcacttt gtatagctgc agatgctgca
     3301 gaagataatt ttaatgcaca accagctttc gcaccgtcac atatcattcc acttaagtca
     3361 gctatcatat tttctatagc tccttctatt tgcttttcag taccattcat aagccaagag
     3421 ataccagcag ttgctccagt agaagcagca acaccacatc cacatacagc agataatctt
     3481 cctgtatagt tcttaatata accagttact agatgagata tagctaaagc ttttgctagt
     3541 ctttcatcag attgtgggaa ttttttatta taagcaacga ttggaagaat tgctgttaat
     3601 ccatgatttc cacttccatt tgaactcata acaggcattt ttacccctgc cattcttgca
     3661 tccgaagctc cagcagttaa catcattgca taatttataa catcattacc taaaagacct
     3721 tcttctatag actttttaat tccatatccg acaccaatac cagttttttg ttttaatcca
     3781 tattcagcca tctcttcatt catttttact ccatctagta aaaattctat atctttaaaa
     3841 tcaattcctt ctacattttt tattaattct tgtatagtta ctgtatccat taaactttca
     3901 gctttttctg ccacatcatt tgaagctgat acttttggtt cattgtctaa taatacttct
     3961 ccatcttttt ctaagaacgt aaaattatca tgtttagttc ttattctcac tttagcaata
     4021 tgattttttc cttttaacac aacttctata aatactttct ctcttgtatc agcaggtgtt
     4081 attgatagtg gagtattatc catataatcc tcagctattt taacttcctc ttttgttaga
     4141 gtttctaaca cacttaatcc attttcagaa tgtccaccta ctatacctag agctgctgca
     4201 atttttaatc ctaatctttc agtacctggt atacctacgc acataccatt tttatagata
     4261 ctaggactaa ctaacatacg attttcaact atttcttctc ctaataattc ctttgccttt
     4321 gcacatgcta gagctaaggc tactggttca gtacacccta ctgctggttt aacttctgcc
     4381 tttaacattt ccactaattc ttttcttata tctctcatta tatataaacc tcccaattaa
     4441 gtttagattt agtactcaaa taattaatta aataatttct tatctatatt tatagcaaaa
     4501 tacatgccat attttttata tctttttata tttttagtct tatattctat aaccattggt
     4561 atgactgctt ctatttatgt atttttttta tttacataga gtatttatat tctttaaaat
     4621 ggataaacat atgacaattt cgcaaaaaga caaaaaaaag tctcaaaatg agattctata
     4681 tcattttgag acggctcaaa tgtattattt tttaacttat aaaaaccatt ttttaattca
     4741 taaaaaaaga ttctgcaaaa cacttatagt ataagtttaa agctttcttt aaagttatga
     4801 atttagaaaa atttaaattt tatatagctt tttattatat tactatgaaa ttatattgta
     4861 tttttcaatt tttctatata atgtagctct tgatattcca agtgctttag ctgttaattc
     4921 tttatctttt ttataatctt tgtacttatt taaagctttt attatctcaa tcttctctag
     4981 ttcatcaagt ggaattatcc tatcgctttt aacttctaac tcttctacag aggtattctt
     5041 aattttttga ggaattacat tcattgtgat tattgaagaa gaagacatat tcacactata
     5101 ttcaataata ttctgcaact ctcttatatt ccctggccac ttatattcca tcatagcatc
     5161 aacaaccctg ctatctatgt caataacatt tttatctaat ttttttgaat actctttaac
     5221 catatgcttg attataagtg ggatatcttc ttttctctct cttaaacttg gtaattttat
     5281 aggtataaca tttaatctat agtataaatc ttctctaaaa gtgccatttt gaaccatttt
     5341 ttctaaatct ttatttgtag cagcaataat tcttacatct attgaaacat ttgatttact
     5401 tccaatttta ttaagttcct tttcttgtaa tactctcaat aactttgact gtaaatgcaa
     5461 actcatatct cctatttcat ctaaaaatat acttccctta tgagctactt caaacattcc
     5521 aagctttcca ccttttttag ctccagtaaa agctccctct tcatatccaa ataattcact
     5581 ctctaataaa ttatccggta tagcagcaca gtttacagct ataaaaggat tatctactct
     5641 attgctatgg ttgtgtatgg ctcttgcaaa tagctcctta cccgttccac tttccccagt
     5701 aataagtact gttgatgttg atttagaagc tattagggct tcctttttag aatttcttat
     5761 agctacactt tctccaatta tgttttccaa ttttattcta taatctttat tcatcttatt
     5821 gtagtttttt atagcatctc ttttatctat aaaatcaata acatatccct ttaactcatt
     5881 ttttaatact attttattga tattgtatat tcccttcgaa tcatatccat atcttttata
     5941 aaaaaaacta caggatttgt ccttcttaaa gttatttata gaaggtttct ttataaaatc
     6001 taattcgaca aatatgtttt tgccagttat actatctttt atattgaaaa gctctttaaa
     6061 ttttgaatta tatttatcaa cttttccatc cacatcaata gacacaacag ctttgtccat
     6121 attgtttaat agtgtctcta attttttctt ttctaattca agctcataag ttttaatttg
     6181 tgcttttaat ttattagata ttaggtctgc cattttgcct aaaaagttta taagaccact
     6241 tttattattt tctataattt gtgcttgttc ttttgtaaag gctataaggc ctataacacc
     6301 atgagaatcg ccatcacata taataggaca acacacctct gcaaactcta cacaagcccc
     6361 acttttatag cattccctac atatatcact actctttggg tcatcaatta gtattgtaat
     6421 ctgttctgta agtgattttt taaaagccgt atatttatct actttctcac ctattttgtc
     6481 tatatatact cctgtacctg caatcctaaa taactcctta tttactatag ttacatctat
     6541 attgagtact gatactatag cttcagctat attttgaata tcagatttta tatattctaa
     6601 acctatcata atcatcattc cttatttttc tttaacataa gtatttgtct tatttcttta
     6661 agaatcaagt atatcttatt taaagtaaat tggattgaca tcaggattac aaagagcata
     6721 acaattatta ttaactcata atttatttcc atctaaacat ctccttataa caaaagacta
     6781 ggcttaacct agtcttttat attatatcca tatttatatt ttaactcatt tatcttcttt
     6841 ttcccacata aatatatttt aatttatttg actctttaat cataccatca aagtttaatc
     6901 ttgaaaatat attaaatact tcatgtggat agtgaatctt tgcaacagaa ccacaaaatt
     6961 tatgtttaag atagtttaac ttaactttta aattctttaa atttaaatcc tctaatataa
     7021 cctctccatt gccctttaac actctttgta tttcagatga agctttttca caatctgtaa
     7081 taacattaaa ttggtcaact attattatct tgttgaactg attatcatca aatggtagaa
     7141 actctaactt acctttaact acattgcaat tttcattata cactagagaa taacagacat
     7201 cttcataatt actattctcc agtatagtta cagaagttcc aagacctctc aatctcttgc
     7261 ctaggtttcc tatatgacca acaagcaata ttttatcacc tacattatac tcaattgtat
     7321 ccaaaaaaca ttctaattca tcagatgtat atttagcttt aaccggctgt ttatttatct
     7381 gacataatat gcttttcaca atgcttcacc tcttcatttc ctcaattata aattatgtac
     7441 agattacatt ataataaatg gcgagcaact attcaagaga tatttcatta gtattttttt
     7501 aaacaatctt tatacatttt aatatgatat tataccaaat ttattaggat tttattcata
     7561 aaaatcagtc tatttttcat gtttattcat tgttattttc atccaatttt tttattttcg
     7621 atatggatac ttcatatgcc atttttttga ccacattatt ttcatctatt tttttctcat
     7681 attctctact ttgaacccta ccccaaagct gtattctatc accaaccttt agattctttg
     7741 caaatttagc atttctaccc caaactattg atggtatata atcagattta ttataaggtc
     7801 tgtttatagc aactaataaa tcggttattt cccttcctaa aggtgtcttc ctatacacag
     7861 gttccttaca tacatatcca tctaaaaata tactattagg gtctttattt tcctcgtcta
     7921 tgctttttat ttctctgaca aacactgtaa gcactaatct atttttatta tttaatgttt
     7981 tgttatatga tcttaattgg cctacgacat tgactaaatt ttgtttatca aggtcaatat
     8041 cttggagaag tctttctgat acagtcattg gaagtatatc aaaagaatcg cttagtctgt
     8101 ttatttttat tttcatatta taaaattttt ctccaaatat ctcatggcta aattctagct
     8161 tattgtctaa ctcccctctt aagtttacta cgttattctc atctttaaca gctatcatat
     8221 tttatccctc cacaattgtt aattcttata tttctggtat cagcttatat aataaatata
     8281 tttataaggg atgtatttta ttacaaatct ttgattgctg tttaaaagtt tttatttagt
     8341 tataaatttc atttaatatt tcacttttat aattataaaa ttattat
//
LOCUS       DAKBNN010000166         8316 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000166 DAKBNN010000000
VERSION     DAKBNN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.167"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            177..1619
                     /locus_tag="OUB02_003016"
     CDS             177..1619
                     /locus_tag="OUB02_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HCT8886752.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1661..2014
                     /locus_tag="OUB02_003017"
     CDS             1661..2014
                     /locus_tag="OUB02_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886753.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2205..3026
                     /locus_tag="OUB02_003018"
     CDS             2205..3026
                     /locus_tag="OUB02_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HCT8886754.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3284..5167
                     /locus_tag="OUB02_003019"
     CDS             3284..5167
                     /locus_tag="OUB02_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCT8886755.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5392..6072
                     /gene="pgmB"
                     /locus_tag="OUB02_003020"
     CDS             5392..6072
                     /gene="pgmB"
                     /locus_tag="OUB02_003020"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCT8886756.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            6069..>8316
                     /locus_tag="OUB02_003021"
     CDS             6069..>8316
                     /locus_tag="OUB02_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCT8886757.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYDEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGK"
BASE COUNT         3345 a          783 c         1524 g         2664 t
ORIGIN      
        1 tgtaactaaa atgtaaattt ttcctttaat ctatacaata ttatggaaac tttttgtaat
       61 ttatgatata ataacaaata tataattatt aaattataat aaatgactat agcgtagatg
      121 taatactatg ttatagtcat ttttgaatta aacttgatat atcaaggggg aagaatatga
      181 gaacaattag aagagtttta gcattaggtt taacattagc tatatttcta ataaatgtac
      241 ctaatgtaga tgcattaaca tcagatacaa ttaaaggtaa caatatatat gagacggctg
      301 gattaatagc agataaaaag agttatgata cagcaattat ggtaaatatg gataattcta
      361 tagcagatgg tctttcagca agtggtcttg caggtgctgt tgatgctcca attttgcttg
      421 cacaaaaaaa taagatacca aatgaaacaa aacaaagatt aaaaaatgtt aaaaagatat
      481 acataatagg taaagaatta tcaataagta agtcagtaga aactgaacta aaaaacacag
      541 gggcacaggt aactagattg ggtggagatg acagaataaa gactagttat agtgttgcta
      601 aagaggtaaa tggtatcaaa aaagttgatg aagtgatatt aactaatgca tataaaggag
      661 aagcagatac aataagtgct gcacctgtat cagtaagaga tatagctcct atagtactta
      721 cagatggaaa gagtgtgcct ttttcaacaa gtaatgtaaa aacatatgca gtaggtggga
      781 gtatatcaat gagcactagt ttagttaata aaactaatgc taaaagactt ggtggttcag
      841 atagatatga tacaaataag aaagttataa aagagtttta tccagatgca tcagaatttt
      901 atttgagtga tggatatgat ttagtaaatg cacttacagg ttctacaatt gctaaggaaa
      961 atccaattgt attggtgtca gaaagtagtg ataagtctat attagcagga gcagataaga
     1021 ttactagatt aggttcaata agtgacagtg tgtataataa gtgtgtttct gctgcacaaa
     1081 ataatggcga ttcgtctaca aaaggtgagt cacctatgaa aaatgagaca agcatattag
     1141 gtcagccaac agctagttta gaagcgtgct taaaatgggc aaaatctaaa aaagctaatg
     1201 atttatttat agagttaata ccaatattat atgatactgc tgttcaggaa ggtgttaatc
     1261 ctgttttggc agtagctcaa tctgcaaaag aaactggttt ttgtaatttt ggtggagtat
     1321 tagatgcatc atttaagaat ccttgtggac ttaaaacttc tgtaggtggt tctgatactg
     1381 ataagaatgc ccattcaaga tttgatactt gggaagaagg aatattagct caaattcaac
     1441 atttatgctt atatgcagga caagatggtt acccactttc aaatccagta gaccctagac
     1501 atgaaaaatc tttatttggt aaggctaaaa cagttgaaag tttatctaat aattgggctg
     1561 gaggtcaata tggacaagat ttagtaagaa tgatgggaga aattgaggca acaaaataat
     1621 tgaagattat caaatatcca ataaataagg attgattatt atgaaaaaga gtaatatttt
     1681 tagtatcata tttcctacag tcataatggt gctaatgttt ttaacttttg ctacagatat
     1741 atttaatatt ccagatattc aatctaaaag tatttttgtt gtgggaatgg ttttgatttt
     1801 tccaatttct tttttgatac aagggataat ttgtgtatta aataagacta attggatttt
     1861 atctcttatt gtgtctttag taacttatat agtattaatg tatatatttt taaatgattc
     1921 tgcatatata tatgttttac tttatacagc tttttatatg attggacata taacagctaa
     1981 actctattac aaaattaaga cttttaaaaa ttaaattgaa gtatataaaa gtaacattaa
     2041 gagaagtatc cttaatgatt ttctaaaatc agaaataggt gcttctcttt tttgtaaaaa
     2101 taaaagtatg taaataaaat ttaaccatta taattttctt ttaacaagtg tatacaataa
     2161 acttgttgta taattaagat agataaaata aagggaggga taatatgaat ttaatagata
     2221 atcatattca tactaatttt tcaagtgatg gaaaggattc catggaagat acaataaaaa
     2281 aagctataag tattggtgtt agatatttga cttttacaga ccatttagaa catgatgaag
     2341 atagaggatt ttgcataaat tataatgact atgttccagt atttaataag tttaaagaaa
     2401 aatataaaaa ggacatagag ttattactag gtgttgaagt gggttacaga aaacatctaa
     2461 aaaatgaaat agaagaaata ataaattcta atccttttga ttttgtattg tgctcaactc
     2521 atacaattga caatgtacct gttccatcaa aggcgtattt taaaggtctt agcaaagaag
     2581 atgcgtatta taaatatttt aatagcatac ttgaaactaa tcgtgaattt ggagattaca
     2641 atatatatgg tcatttggat tatatatcta ggtatggaat atattcagat aatagagtta
     2701 tatacaatga ctttaaagat gttattgatg aggtcttaaa gtcaataatc aataatggga
     2761 gtggtataga gctaaatact tctggttata gatatggact aaatgctata catccaaatg
     2821 aagatattct aaaaagatat aaagaacttg gaggatttat agttactgta ggttctgatt
     2881 ctcatagggt agaagatata tgcaaagatt ttgatgtggc ctatgatatg cttaagtatc
     2941 ttgactttaa atatgtatct ttatttaaag aaagagaaac ttattttgta aatatagaaa
     3001 aagttaaatc taacaatata gcttagagtg tgttttgaag taaattaatg gatagttaaa
     3061 aagttaatag ttataatatt gtatcctcta gaaaaatact agaggttttt attttgctaa
     3121 aataactaaa tagaattaaa tttaaatagt atagcttatt ttaaattgaa tagtttattg
     3181 ttgatatatt ttactattat atatttttat tgtgatataa tagtaaaata tattaaccta
     3241 tctttagatt atataaatta ttataaagga gggaaatttt aaaatgtcta ataaaaataa
     3301 agaaaatata actttaagta atcaaatcaa aggaacaatt caaaatagtt taacaaaaga
     3361 agctatatta catataccaa tgagcaatta tgcctatggt tatgatagag aaacactaca
     3421 tataagactt agaactaaaa aaaatgaagc gaaaagagta attttacgaa taggagacca
     3481 atatgtgtgg gataaaggag gtgcaggagg aggaaattta aatgcatctg gagttggttg
     3541 gtctggtgga acaaatatag taatgagtaa agaagtagaa actgaattat ttgactattg
     3601 gatagctaag tgtaagcctt taaataaacg ctcacgatat ggctttataa tagaaggtca
     3661 agaggaaaaa cttttattta cagaaaaaag aataatagag ttgggaaatg aaaatgatga
     3721 aaaggaatta tgtgaaatag gtaatttttt tggatatcca tatttaaatt atatagatat
     3781 acctaaagtg ccaaattggg tgaaagatac tgtttggtat cagatatttc cagatagatt
     3841 tgcaaatggt gacccgtcta taaacccaga aggagtagat aaatggggag ctattccaac
     3901 cagagataat tttacaggtg gagatttaca aggtgtgata gaacacttag attatttgag
     3961 tgatttggga ataaacggaa tttacttttg ccctattaca attggaaaaa ccaatcatag
     4021 atatgatacg gtagattata tggaggtaga ccctacatta ggagataaag agactttaaa
     4081 aaaactaata gaagaagcgc ataagagaaa tataaaaata atgttggatg ctgtttttaa
     4141 tcatatagga tattattcta aacagtggca agatgtagtt caaaataagg aaaattctcg
     4201 atataaaaac tggttttata taaaagatat gagtaaaatt gatactccaa tagaacaaat
     4261 agatgaaaaa aatatacctt atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa
     4321 tacagaaaat tcagaagtaa taaaatattt attggatgta ggcaagtact ggatacagga
     4381 atttgatata gatgcgtgga gattagatgt ttcaaatgaa gtagaccatg ttttttggag
     4441 aaagtttaga gaagaagtca aaaaagtaaa accagacatt tatatattgg gagaaatttg
     4501 gcatggtagt ttaccatggc taatgggtga ccaatttgat tcagttatga attacttgat
     4561 gtcagaagcc atgaaaaaat tcttttgtac agatgagata aacgccgaag aatttaagta
     4621 tatgataaat gatgtaatgg taagttatcc aatacaagtt agtgaagtta tttttaattt
     4681 acttggaagt cacgatacta caagaatatt gacttatgct aatggaaata ttgatagatt
     4741 taaacttgca tatatgttta tgtttgtaca aacaggatgt ccttgtatat actatggtga
     4801 tgaaatcgga atggaaggag aattaagttc aatatctgaa ggtcaaagaa aatgtatgga
     4861 atggaatgaa gaaaaatgga ataaggatat atttgatttt atgaagaaga ttattagact
     4921 tcgtaaagaa aataaagaac ttaggtctat atcaaatgaa tgggtgttag cagataagga
     4981 aaatggaacg attatattga aaaaagaatc tatttctata ataataaata attcatctaa
     5041 aaatataatt ttgacgttac cagattattt agcaaataaa aaagtaaaag atttatatga
     5101 agaagaaata gttgatttaa aagaagaact aatgttagag aaatacaagt tcattatttt
     5161 aaaataatac aaaaaagtat ttattttaga tttcatgtta aattattata cagtttaaat
     5221 aagtctttat aaacgataaa aaaatatggt ctacaagtga gtgaattgaa agtagatttt
     5281 aaattaaaga ctgagttaga gtagataatt ttataaataa tatcttaaat acaagataga
     5341 aaataaattg attattagtc taaaaatttc ttgtagaaag aggaaataag aatgattgaa
     5401 gcatttatat ttgatttaga tggtgtaatt acagatactg catattatca ctatatggct
     5461 tggagaaaac tagcccataa agttgggata gatatagaca caaaatttaa tgaatcttta
     5521 aaaggcataa gtagaatgga gtcattggac aggatattag aatttggaaa taagaaatat
     5581 tcattttcag aagaagaaaa agtgagaatg gctgaagaaa aaaataatta ctatgtatca
     5641 ttgatagatg agataacttc aaatgatatt ttgccaggta tagaaagctt gttaattgat
     5701 gtaaaatcta ataatataaa gataggtcta tcttctgcaa gtaagaatgc aataaatgta
     5761 ttaaatcatt tgggtataag tgacaaattt gattttatag ctgatgcagg aaaatgtaaa
     5821 aacaataaac cacatccaga gatatttctt atgtcagcga aagggcttaa tgtaaatcca
     5881 caaaattgca tagggataga ggatgcaagt gcaggtatag acgctataaa ctcagctaat
     5941 atgttttctg ttggtgttgg aaattatgaa aacttaaaaa aagcaaattt agttgtagat
     6001 tcaacaaatc aattaaagtt tgaatatata caagaaaagt ataatgagta tatagttagg
     6061 agaattatat gaaatgttta tttagtaatg tagatgagtg gaagattata caagataaga
     6121 ttgaaatcaa agaaaataga ttagcagagt ccataatgag tataggcaat ggatatatgg
     6181 gtatgagagg aaactatgag gaaaattata gtggagatac acacaagggg tcttatatag
     6241 ctggagtatg gtttccagac aaaactcgtg ttggttggtg gaaaaatggg tatccagaat
     6301 actttggtaa aattcctaat tcagtcaact atataggtgt aagaattttc atagatgatg
     6361 tagaattaga tttagcaaaa tgtaaagtag aagattttta tagagaatta gatatgaaaa
     6421 atggtatttt aaatcgtagg tttattgtaa atataaatga taaaaaattt gaagttaggg
     6481 ttgctaggtt tttaagtata tctgttaagg aacttgcagt aattaaatac agtataaaat
     6541 cattaaattc taatactaaa ataaaatttg taccatattt agattcaaat ataaaaaatg
     6601 aagattcaaa ctatgatgaa aagttttgga atggaatttc ttcaagttct ttagaatata
     6661 aaggtagtat tctttctcga acaaaggaga atgcatttaa tacaccagta ttcactgtgg
     6721 gttcaatgat gtatattaag acaaatggtg atgtagaagc tgtatctcat gagagtgatt
     6781 ataattactg tgaaaattcg ataacaatgg atgttttaaa aaatgaggtt gcaagtatag
     6841 aaaaatatgt atctataact tcaactagag attataagga aagtgagcta ttaagaaaat
     6901 gtgaagacat tttaaataga gaactatcta aaagttatga tgatatatta aatgaacata
     6961 caaagttgtg gaataaaaga tgggaaagtg cagatgtaaa aataaatgga gataaaagta
     7021 gccaacaagg tataagatat aatttattcc aactattttc aacatattat ggagaagatt
     7081 cgcgattaaa tataggtcca aaaggattta ctggtgaaaa gtatggagga gctacttatt
     7141 gggatacaga agcgttttgt gttccagtat atctaggtgt agcagataag gaggtaacta
     7201 gaaatctttt agaatatagg tacaatcagt tagaacaggc aaaggataat gcaaaaaaac
     7261 tagggcttaa aggtgcttta tatccaatgg taacatttga tggagtagaa tgtcataatg
     7321 aatgggagat aacatttgag gaaatccacc gaaatggaag tatagtatat gcaatatata
     7381 actatacaaa ttatacaggt gactataaat atataaaaga aaaaggaata gatgtaataa
     7441 tagaaatagc tagattttgg gcaagtagag ttcatttgtc tacaaaaaaa gatttgtata
     7501 tgatacatgg agtaacaggt ccaaacgagt atgaaaacaa tatcaataat aattggtata
     7561 ctaattatat agctaaatgg tgtttagatt atgctgtaga aaatatctta cgattagaga
     7621 aagagtgtaa tgaatgtatt gacagaaata atgtgactag ggaagaagtt gagttttgga
     7681 aaataatatc aaataaaatg tatctaccgt atgatgagga actacaaata ataattcaaa
     7741 atgataattt tttagataaa gaatttgtag aagtaaagga tttaccaaaa gaaaattttc
     7801 ctcttaatca aaattggtct tgggataaga ttcttaggtc gtgttttata aaacaagcag
     7861 atgtattaca gggaatttac tattttggaa atagatttac taaagaagaa aaaaagagaa
     7921 attttgactt ctatgaaaaa tatactgtac atgaatcttc tttatcttct agtatttatt
     7981 ctattattgc ctcagaaata gataacctag aaaaggctta tgaattatat tcaagaacag
     8041 ctaggcttga tttagataat tacaataatg atacaaatga tggactgcac ataacttcaa
     8101 tgtctggttc atggctttcc attgtacatg gatttgcagg catgagaaca tggaatgaaa
     8161 ctctatcttt tgaccctaag ttgccaaaag agtggaaaaa ttattctttc aatataaatt
     8221 atagagataa tattattaat gtagatgtat gtagtgattt aattaaaata gaaaatttaa
     8281 aaggtagacc aataaaaatt attgtatatg ggaaaa
//
LOCUS       DAKBNN010000167         8310 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000167 DAKBNN010000000
VERSION     DAKBNN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.168"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..654
                     /locus_tag="OUB02_003022"
     CDS             <1..654
                     /locus_tag="OUB02_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCT8886758.1"
                     /translation="EESRIKYTLESILDIKQINEIIVVDDGSIDNTAKVVSELENEKI
                     KFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEKLIVPILNGEADVT
                     IAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKREVLEKFDEIPFGYG
                     VEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHIKKVLGQKKKEWR"
     gene            662..1486
                     /locus_tag="OUB02_003023"
     CDS             662..1486
                     /locus_tag="OUB02_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCT8886759.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            1491..2558
                     /locus_tag="OUB02_003024"
     CDS             1491..2558
                     /locus_tag="OUB02_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HCT8886760.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            2560..3096
                     /locus_tag="OUB02_003025"
     CDS             2560..3096
                     /locus_tag="OUB02_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCT8886761.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            3164..3769
                     /locus_tag="OUB02_003026"
     CDS             3164..3769
                     /locus_tag="OUB02_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HCT8886762.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVIGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            3781..4695
                     /locus_tag="OUB02_003027"
     CDS             3781..4695
                     /locus_tag="OUB02_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8886763.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICANNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            4737..5909
                     /locus_tag="OUB02_003028"
     CDS             4737..5909
                     /locus_tag="OUB02_003028"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCT8886764.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            5956..6768
                     /locus_tag="OUB02_003029"
     CDS             5956..6768
                     /locus_tag="OUB02_003029"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCT8886765.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            6972..8297
                     /locus_tag="OUB02_003030"
     CDS             6972..8297
                     /locus_tag="OUB02_003030"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCT8886766.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDTPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT         3306 a          824 c         1577 g         2603 t
ORIGIN      
        1 gaagaaagta gaataaaata tacattagaa agcatattgg acattaagca aataaatgaa
       61 ataatagttg tggatgatgg ttcaattgac aatactgcta aggttgtatc tgagctagaa
      121 aatgaaaaga ttaaattttt taagttagat aaaaatagag gtaaaggata tgctttaaat
      181 tatggtatca aattagcaat gaaaaatgca gatattatag gttttctgga tggagattta
      241 ggaagttctg ctaaagaagt tgaaaaatta atagtaccta tattaaatgg tgaggcagat
      301 gttacaatag ccaagtttcc tcctgcaaag agaaaaggag gtttgggatt tgtaaaaggt
      361 ttagctaaaa attctgtgtt tgagatgact ggtgttgagt tagatgcaac tttgtcaggt
      421 caaagaatat ttaaaagaga agttttagaa aagtttgatg aaataccttt tggatatgga
      481 gtagaagttg gaatgaccat tgatatatta aagtatggat ttaaaattaa agaagtatta
      541 gtaaatatga ctcataatga aacaggaaga aatttaaaag gttttattca tagagggaaa
      601 cagtattatc acataaaaaa ggttttagga caaaaaaaga aagaatggag gtaatatctt
      661 agtgaaagat tatatacttt ataccatttt attattaact ggattatttg gaacctatgc
      721 tgtaattcca ctatttaaaa atttattgat aaatggaaat gtacttagac caaactataa
      781 aaaagatatg ataccagtaa gtatgggaat tgtatttttg cctatgattg taatcaatgg
      841 aataatactt gctttcttta ctacagaatt taaggattta gcgtatatat ttatgtttat
      901 gtttggtatg atatccatgt tttttgcagg gatactagat gatgtcattg gaaatagaga
      961 tgtaagtggt ttaaaggggc attttaaaag tttatttaaa ggaagtctta caacaggagg
     1021 ttttaaagct ttatttggtg gatttgtagg gcttatagtt tctgttgcta tatcaaaaga
     1081 cataattgat ataatagtaa atacactaat aatagctcta tcaactaatt taatgaatct
     1141 tttggattta agaccaggaa gggcaataaa agcatattta ttcataatgg taattatatt
     1201 tttaacattg acaggatttg ttcaagttct tccattactt atagtaccaa atgtacttgc
     1261 atactttaat tatgatttaa aagcaagggc tatgatggga gatactggtt caaacgtcct
     1321 tggaatatct attggaatgc tcatttcttt tgggtatcca tttaacatta gattaggatg
     1381 gttaatattt gtagttatta tgcatatatt cactgagaaa tactcattaa ctaagataat
     1441 agaaaaaaat aaactattaa attttataga taaattggga aggtagtatt atgataagca
     1501 aaagagtggg gattgttgaa tcaatagtaa gtcaaactga aactctagat gatataagag
     1561 taaatataaa tggagaaata caaagggcat ataattatcc caaaatatct ggaactataa
     1621 atataggtga cgaagtggtt ttaaatacaa cagcagtaga actgagctta ggtacaggtg
     1681 gataccattt tgtaataact aatttaaata atatagaatc tactttaact gaaggtggac
     1741 atataatgaa acttagatat acgcctcttc aaataaaagt ggattctgtt gaggaacaag
     1801 aaagtattta tcatgataaa tttgcgaatt ttaaaggatt agaaggtctt tctgttgtag
     1861 tagggactct tcatagtatg ttaactccat ttgcagcttc atttaaaaga aataatccaa
     1921 ataaaaaatt agtgtatata atgactgatg gagcatctct tccaatttat ttaagtaaaa
     1981 atgtggatac tttaaaagaa aaaaaattaa tagatagtac aataactata ggaaatgcgt
     2041 ttggtggaga ttatgaatgt ataaatatat atactgcatt aattacagca aaagaaatac
     2101 taaaagcgga tgcagtattt gtcagtatgg gacctggaat tgctgggaca ggaacaaaat
     2161 atggatttac agggattgaa caaggctcta ttcttgatgc cgtaaaaaaa cttgaaggaa
     2221 gagcaattgc aattccaaga attagttttg ctgacaaacg agaaagacat caaggtattt
     2281 cacatcatag tatgactgta tttaaagaaa ttgtaaatgt aaatgtagac ataccaataa
     2341 ctatatatga tgatgaaaaa ttgaacttta taaaagagca aatcaattta aatggtttag
     2401 acaaaaagca taatataatt tatatagata ataataaaac gaaagaggat ttagactatt
     2461 ttaatctaaa agtaaagagt atgggaagaa attatgaaca agatgaggca tttttcaaag
     2521 ctgcaagcac agcagcatat tacttaatgg aggtttaaga tggtattaga agaaaaaact
     2581 attagtagtg atagggttta tacaggtaaa gtaattactt taaaggttga tactgttgaa
     2641 attccagggc aaggatatca aaaaagggag ttagtagaag ttggtggagc agtaggaata
     2701 gttgcaatca cagatgataa taaagttgtg ttagttaagc aatttagaaa acctatagaa
     2761 aagccaatct ttgaaattcc agctggtaaa ctagaaaaaa atgaaagtcc aaaagaatgt
     2821 gctgaaagag aattaaaaga agaaactggg tatagtgcaa aaaatattaa gctaatacat
     2881 aaatttttca cttcagcagg tttttctaat gagataatgt ttgtatattt agcaacaggt
     2941 cttactccag gagagaataa tttagatgca gatgagtttt tagatgtcta tgaaattgag
     3001 cttgaagaag catataacat ggtattaaaa aatgacgttg aagatgcaaa aacatctatt
     3061 ggattattat tagtaaaaga tatgtttaaa aattaatatt tataaaatga ataacccttt
     3121 gtcctaaagc atactatttt ataaaggaca agggggaatt aaattgagaa gaacatatag
     3181 aaaaaatgat tttagagaat taaatagaca aataattatt ataggattgt tgtttatgat
     3241 gtctattgtt attgggtctt acataaataa aatattgcca ggttctagta acaatatatt
     3301 aaacaatata aatcctgctg tagagtatta taatctaaat ataagcataa aagatacagt
     3361 gatacaaaat ttaaaatcag atgcaatctt tatgggctca atagctctat taagtctatt
     3421 tgtagtaaca attccagcag tgttggtagc ttttgtatta aaagggatgt caattggtta
     3481 tacaataaat agctgtatat tggcattaaa atttaaaagt ataaaaatga ttttaataat
     3541 attatttaaa aatttgatta ttattccatg tgcaatcata ttagcattaa ttagtttaag
     3601 ttattttaaa gaaatggttt atgaatttaa aaagaaaaat agaaaaaata tgcaattttt
     3661 aattaagagg tatatactaa atattataat tataatagca ctatcattgg ggttgcaatt
     3721 aatattaaat actgcaagta ttggtattat taagttccta gctaaataaa ggagtttata
     3781 atggatatta tagagggata tatagattac ataaaagata aaaaaaaatt atcagagaat
     3841 acagtggcat catactttat ggatataaaa aaatatatgg aatatataaa tcaaaaaagc
     3901 attaaactag tagatatagt agaaaatgat attataagtt acttaataaa gttggaaaaa
     3961 gataatgtat caatagctac aatagcgagg atgatatcct ctataaaatc ttttcatgat
     4021 tatttatttt taaatcatat atgtgcaaat aatcctgcaa aggatataaa aaaacctaaa
     4081 ataaaaaaag aaaatataaa tatacttaca gaagaagaaa ttgaaagact attaaatttt
     4141 ccaaagttaa caacgcctaa attgattagg gacaaggcta tttttgaagt attatatggt
     4201 actggtataa aagtatctga attagtagag atgaatatag aggatattga cctagatatt
     4261 gattatatat attgtaattc atgttgtaaa aatcaaagag tgataccact ttgtgatatt
     4321 acaaaacttt acttagaaaa gtatctaaaa gaagcaagac ctaaaatggc tgtagaaggt
     4381 gaaaaatcat tattcgtaag ttcattaggt caaaggttta cacgacaagg tctttggaaa
     4441 gtcataaaaa aatattctaa tctagccaat atagataaaa atataaatcc tacaatgctt
     4501 agacattcat ttgctattca tttgttaaat gaaggtgcta acattgctgt tgtaagtaaa
     4561 attttaggaa atgttaattt atctagtttg caagtatatt taaatcatat agataaaaac
     4621 gtgagaagag aaataaagga gaaacatcct agaaatgatg ttgatgtaga attacaaaaa
     4681 gcagaagcta aatagactat atagcaaatt ttataattta gaaggagcat atttatatga
     4741 gcagagtaat ttggattgta attgatagtg ttgggatagg agcattgcct gatgctgaaa
     4801 aatttggtga tagtaaggat gtaagtaccc ttggaaatat atttaaggaa tatccagata
     4861 ttcagattcc taatatgaga aatttaggta taggaaacat agatggtata gatttttttg
     4921 aaagtataaa agaaccaata ggttgttttg gaaagtgtaa ggaaatgtct cagggaaaag
     4981 atactactac aggtcattgg gaaatgactg gtataatagt tgacaaacca tttaagacat
     5041 ttgaacatgg attctctaag gagataatag aagagtttga gaaaaaaact ggaagaaaag
     5101 tagtaggaaa taaaccagct tcaggaactg taataataga tgagtatgga gagcatcaaa
     5161 taaaaactgg agatgtaata gtatatacat cagctgatag tgtatttcag attgctgcta
     5221 atgaagaagt aattccccta gaagaactct ataatatgtg caaaatcgct agggaaataa
     5281 tgatggggga taatgcagta gctagagtaa ttgctagacc attcataggt aagaaaaaag
     5341 gtgagtttgt gaggacttca aatagaagag attattcact agacccattt gaaccaacag
     5401 ttcttgataa tataaaagaa tcaggacttg atgttttagc agttggaaaa atagaagata
     5461 tttttaatgg aaaaggaatt acagatgcta tccacactaa aagcaatatg gatggagtgg
     5521 atgaaacttt aaattatatg aaacaagata ataaaggtct aatatattca aatctcgttg
     5581 attttgattc taaatatggt cataggagag acccagaagg atataagaaa gctcttgaag
     5641 agtttgatag tagacttcct gaaataatgg ctaatatgag agaagatgat attttaataa
     5701 tcaatgcaga ccatggaaat gacccaacat ataaaggtac agaccataca agagaatata
     5761 tacctgttat gatttatggc aataagatta aaaaaggttt taatttagga gtaaaagata
     5821 cttttgctga tattggagca actgttgcag atatactaaa tgtaaagttg cctaaacatg
     5881 ggtcaagctt taaagaagat ttattttaaa ctataaaatg ataaaaactt agcttaatag
     5941 gatggagaga ttggaatgtt tgaaaagata gcacaaagta gtaaatttat aaattctaaa
     6001 agtaatataa aacctaagat tggtttgata ttaggttctg gactaggtga tttagcaaat
     6061 gatatagaag accctgtcac aattaaatat agtgaaattc caaacttccc tgtatcaaca
     6121 gtagcaggac atgctggaca acttgtaatt ggaaaacttg aaggaaaaga agttattgct
     6181 atgcaaggta gatttcatta ctatgaagga tattctcaga aagaagctac ttttccagta
     6241 agggttatga aagaattagg agtcgagatt cttatagtaa ctaacgctgc tggaggcgta
     6301 aataaagaat ttaaagcagg agatttgatg cttataagag accatataaa ctttagtgga
     6361 agtaatccat tagtaggaaa aaatgatgac agatttggtg ctagatttcc agatatgtca
     6421 gatgcatatt cttctaaata tgtagatgta gttaaaaact gcgctaaaga atgtaatata
     6481 gatgtcaaag aaggcgttta tatgtttttt agtggtccaa actatgaaac accagcagaa
     6541 gtgagaatgg cacaaatttt gggagctgat gctgtaggta tgtctactgt tccagaagtc
     6601 atagtggctt ctcactctaa tataggagtt attggtatat cttgtataac aaatatggct
     6661 gctggcatct taaatcaacc actaagtcat gaggaagtaa tagaaactac tcaaaaagtt
     6721 aaatcagaat ttatgaattt agttaaaaca acggttaaaa atttataagt taaagcttat
     6781 tatagatttt ttgaggaagt ttataataaa atagttaatg tttaatttga aaagaattaa
     6841 taaaaagctg tcttgatata gaaagttatt tctaataaag gacagttttt ttataatact
     6901 acaacatttg aaagtgtttt aaagtttaat atttatttaa attattatag gttagaatgg
     6961 agatttcaat tatgagaatg tatgatatta taaaaaaaaa gagagataac ctagagctta
     7021 gtaaaaaaga gataaactac tttatagaaa actattcaaa gggagataca cctgactatc
     7081 aagcttctgc actattaatg tcgatttact taaataagat gaataaacag gaaactgtac
     7141 atcttacaga agcaatgatg aactctggag acatgataaa cctatctgag atagatggaa
     7201 taaaggtaga taagcatagt actggtggtg ttggagataa gactaccata gctcttatac
     7261 ctttggttgc ttcatgtgga gcaccagttg caaagatgtc aggtagaggt cttggtcata
     7321 ctggtggtac tattgataaa ttggagtcta taccaggttt ttcgacagaa atggagataa
     7381 gtaaatttat tgattctgta aataagtcta aaatagcagt ttgtggtcaa agtgcaaagg
     7441 tagcaattgc agataagaaa atatatgctc ttagagatgt tacagcaact gttgataata
     7501 tatcattgat atcatcaagt gtaatgtcta aaaagttagc ttcaggagct gatgcaattg
     7561 tgctagatgt aaaaactggt gatggagcat ttatgaagac attagaagag tcttttgagt
     7621 tagctaaatc tatggttgat ataggtactg gtatgaatag agatacaata gggattgtaa
     7681 cagatatgga tgaaccttta ggttttgcag tgggaaactc tttagaagtt atagaggcaa
     7741 ttgagacatt aaaaggaaat ggacctaaag accttgttga gctatgcgaa gtattaggag
     7801 catatatgtt ggttttatca aaggtagcac atgattttga agatggtgtt gaaaaaataa
     7861 gagaatcaat aaaatctggt actgctatac aaaaactcaa agagtttata gagaatcaag
     7921 gtggtaataa agatgtagta gatgattatt ctttattcaa aaaatcaaag tataaatatc
     7981 cagttaaatc aactaaatca ggttatataa gcaaaattaa agctgaagat gttggtgtat
     8041 cggctatgat tcttggagca ggtagagaaa ctaaagatga tattttagat ttatcagcag
     8101 ggataatttt agaaaagaaa gtaggagatt atgtaaatga aggtgaagtt ttagcttaca
     8161 tgtattataa tgatgaacaa aagctttcat tggcaaaggg aagatttatt gattcctact
     8221 ctattgtaga ttcaaaaata gaaaaaaata aattaatata tggtattgta actaaagaag
     8281 agattaaaaa gttttagaaa agtgattata
//
LOCUS       DAKBNN010000168         8221 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000168 DAKBNN010000000
VERSION     DAKBNN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.169"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..327
                     /locus_tag="OUB02_003031"
     CDS             <1..327
                     /locus_tag="OUB02_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886767.1"
                     /translation="WLDNVVGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNI
                     KPCNMTLDYTRVLISKDNYMNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGY
                     QEVTIF"
     gene            339..1364
                     /locus_tag="OUB02_003032"
     CDS             339..1364
                     /locus_tag="OUB02_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCT8886768.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEVRSETRS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKLSGNLSDIATSV
                     NELITYC"
     gene            1381..3042
                     /locus_tag="OUB02_003033"
     CDS             1381..3042
                     /locus_tag="OUB02_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886769.1"
                     /translation="MATEWNFDFKAEAQPITLKAGKYKLECWGAHGKVWGGDSQSSGG
                     YSYGELTLKKETTLYVYTGATGSSNKYEKFTFNGGGLGVNNGGGGATDIRLVNGDWNN
                     EQGLLSRIIVAGGGGDAFSKTPAGKGGGFKGGNSTNDDNSSMLIVPGGTQYDGGRGYC
                     DEWDGVFGCGGGSILGLERGKYPYNSGGGGWFGGAGARNTSSGGGGSGYVLTKDSYKP
                     VGYIPTSEYWLENVGSITGGNTAKVNGYAKITLLQALPILTISSYNSTQATFKADHTD
                     PTLLTKIEYFIDDVLKETITTDLTLEKTINYTLEDNALHTLKIVVTDSNNATAEKVLS
                     ISKNIMPLPGENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELSE
                     KIKTSFDSSDASVQDLMNQLTRANNTISQLNTRYKVASGITYQLNNPSLSANFYNGGY
                     TTTQDHWINVSNLGFVPHIFIAECDFTKDGYLTKSLVFASYNVFSKDYVISSYFRRQT
                     NSTFYSHGNIYNLNEKDVYVNGRGVQLPAFNNYDFAYKWQAIKFV"
     gene            3056..3349
                     /locus_tag="OUB02_003034"
     CDS             3056..3349
                     /locus_tag="OUB02_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886770.1"
                     /translation="MNRANRIIYDQTGKMLLQTGEATGDVLEYDTITELHCIDIPYGS
                     IDYTKNRIIGINIETKEPILEEIPIFVTEEEKRIQELENQLLLNENEKVGGLL"
     gene            3349..3531
                     /locus_tag="OUB02_003035"
     CDS             3349..3531
                     /locus_tag="OUB02_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886771.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNVEYRKAVED
                     YIIRESGVVEEAEPTI"
     gene            3564..3755
                     /locus_tag="OUB02_003036"
     CDS             3564..3755
                     /locus_tag="OUB02_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886772.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENLFESDNVEGALKELATE
                     VNGQRAKGITIANDLIDMI"
     gene            3769..4401
                     /locus_tag="OUB02_003037"
     CDS             3769..4401
                     /locus_tag="OUB02_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886773.1"
                     /translation="MTEKLTDNASLRELMTTLQGVQTDFQNGKSNITTVLGSPFLNTD
                     KLDITKTKIETLKSALVNNLIYKGVSASTQSSFTDLINAINFIVQSILIVKCKGTGRI
                     EEVNNPYVIYNDKPNIKGCLRIKGTLEVYKNWATFASARLEIIYGEKKEYIYLSCDAT
                     ASGRSTNFEKDIIVNNDTQLKIQVLLTDVGTGNSSTAKAYTSVSDVTLLR"
     gene            4412..4615
                     /locus_tag="OUB02_003038"
     CDS             4412..4615
                     /locus_tag="OUB02_003038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886774.1"
                     /translation="MKKQFYYDSLEEKEKIINENNNLYVIELSETLKGKYIILSDNPI
                     YEKLSYEELENELLTNELKGEIL"
     gene            4869..5414
                     /locus_tag="OUB02_003039"
     CDS             4869..5414
                     /locus_tag="OUB02_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886775.1"
                     /translation="MKDELQIGKNNIAGTLGSPFVGTDKLSVTKTKIQTLKNTLAISL
                     SMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGEKTFQLYQVDNNTPKTCSANA
                     ISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITAVPQPSNFGEMYMPNTFDCVV
                     GHTCILPVKDYKIPHFFYAIA"
     gene            5438..5743
                     /locus_tag="OUB02_003040"
     CDS             5438..5743
                     /locus_tag="OUB02_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886776.1"
                     /translation="MTEYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYIDYDKYKIKGINIETKQPILEEIPIYMSEEEKRIQELENQILIAENEKVGGIL"
     gene            5743..5907
                     /locus_tag="OUB02_003041"
     CDS             5743..5907
                     /locus_tag="OUB02_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886777.1"
                     /translation="MNINNIVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQKIK"
     gene            6107..6742
                     /locus_tag="OUB02_003042"
     CDS             6107..6742
                     /locus_tag="OUB02_003042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886778.1"
                     /translation="MIEKLNANASLSDLITAFENSTNEIKTISNTLKQTLINKNIKVL
                     DTDKLSSLVDKVDKLKAQEFGVRINKLDENPDTCVSYLGDAIGMTPASVGSYGSWDTI
                     DFIRNIKPCGFKDGIVTKYIKKENFNMYEDGTNVEDDTDVMIEFPKFYWKIQSSDNYM
                     DIFISKTKLDDDYECPAHLIGSTEKEFIYIGAYLASLENNNLRSKKIVAQS"
     gene            6844..7455
                     /locus_tag="OUB02_003043"
     CDS             6844..7455
                     /locus_tag="OUB02_003043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886779.1"
                     /translation="MFKSINFFRLGVGYSSYISGHGKPDKINTGGSNFKGMIYGEQNG
                     DEQMKFLGIEDLWGNCLQEVGRCTCGVENACVYTRIITRDKRNIQYGVGRKVGTISDV
                     IGGNNTGFMVKQCLPGGGNGQDSRYYRCWANLSENHIGYYTGFDYNENFRYYGLFSSY
                     FQTEVKKASTRLIFIGEDNQNTLKTIQEDELLFVENEKVGGIL"
     gene            7455..7634
                     /locus_tag="OUB02_003044"
     CDS             7455..7634
                     /locus_tag="OUB02_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886780.1"
                     /translation="MIDGVVVRILADRILNKGLNPLKNRVFELDDVTNTEYRIAVENY
                     IIEHSEVVEGAELTK"
     gene            7675..7905
                     /locus_tag="OUB02_003045"
     CDS             7675..7905
                     /locus_tag="OUB02_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCT8886781.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            7925..8182
                     /locus_tag="OUB02_003046"
     CDS             7925..8182
                     /locus_tag="OUB02_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCT8886782.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQFKDGEK"
BASE COUNT         3312 a          874 c         1551 g         2484 t
ORIGIN      
        1 tggctagata atgtagttgg agaaggcaat tatgaattaa ctattaataa tgctacttat
       61 aacatacacc ttgagagtga tgctagaaat caagattggt ttgaggaagt tcattctttt
      121 gtaagtaata ttaagccatg taatatgact ttagattaca ctagagtgct tataagcaaa
      181 gacaattata tgaattttgg tataacaacc ttaatgggtc aagaaataac tatataccct
      241 tggagtccac cagatataga aacttatgga gaaattgatg tattaactgg caatggagtt
      301 ggataccaag aggtaacaat attttaggag gtgatatatt ggctatagat aaaagttatt
      361 acactataat tacagatgta gggaaagcaa agatagcaaa tgcaagtgtc acaggtaata
      421 aagtgggatt tgtaaaaatt caacttggtg atggaggagg gagtgaatat actccaactg
      481 agagtcagac agctctcaaa aatgtggtat gggaaggcaa tatcggaaat acaactacag
      541 atgaaactgc accaaattgt ataatattag agagtttaat accatcaagt gtaggcgggt
      601 ttatgataag agaaatagga tatttagatg atgaaaataa tttaattgcc atttctaaat
      661 acaaagagtg ttataaacct tctatagaac aaggtgcagt ggtagacatg aaggttaaaa
      721 ctgtgcttat tgtatctaat gtaaataata tagaacttaa aattgaccca acaataatct
      781 ttgcaacact caaagatata caagacttag aaactaaaat aggtactgtt aatactaaaa
      841 ttgatacaac taaaacagaa ttaacaagca acatagaaac tactaaaaca gagctaaaca
      901 ctagaattga cacagaaaat gagaaacaaa atattaaaat tgaccaactt attgcaggtg
      961 gctctaatgt ggcatctact caaacaataa caattgacga ttgggttgag gatgcagaaa
     1021 atggattcaa agcaactgta acacatagtt tattaacaca gagaatagtt gtaaatatta
     1081 tagatgctac tacaaaagaa aatgtagtta caaactttaa aattatagat gataattcta
     1141 tagaagttag aagtgaaaca aggtcagaat taaacgttta tgtgataaat ggaaatgcag
     1201 aaactcattt tataaatgcg actgtagatg ataacagagt atctgaaatg actacttatt
     1261 cgtctaagaa aatcgaagat agattggtta atatagaaga aaaattgagt ggaaatttat
     1321 ctgatattgc aacaagtgta aatgagttga taacttattg ttagagagga gagtgagaaa
     1381 gtggctacag aatggaattt tgattttaag gcagaagcac aaccaattac tctaaaagca
     1441 ggtaaatata aattagagtg ttggggagct catggaaagg tttggggtgg agattctcaa
     1501 tctagtggag gatactctta tggggaactt acattaaaaa aagaaactac attatatgta
     1561 tatacaggtg ctactggttc tagtaataaa tatgaaaaat ttacatttaa tggaggagga
     1621 cttggtgtta ataatggagg aggtggagca actgatataa gacttgtcaa tggagattgg
     1681 aataatgaac aaggtctttt gtctaggata attgttgctg gtggtggtgg agacgcattt
     1741 agtaaaactc ctgctggcaa aggtggtggt tttaaaggcg gaaatagtac taatgatgat
     1801 aacagttcga tgcttattgt acctggtggt acacaatatg atggaggtag aggttattgt
     1861 gatgaatggg atggtgtttt tggttgtggt ggaggttcta ttcttggttt agaacgaggt
     1921 aaataccctt ataattcagg tggaggtgga tggtttggtg gtgcaggtgc tagaaatact
     1981 tcaagtggtg gaggtggaag tggctatgta ttaactaaag atagctataa acctgttgga
     2041 tatataccta catctgaata ttggttagaa aatgttggta gtattactgg gggtaatact
     2101 gctaaagtaa atggttatgc taagataaca ttactgcaag cattaccaat tttaactata
     2161 tcttcttata attccacaca agcaacattt aaagcagacc acacagaccc tacactatta
     2221 actaaaatag aatattttat agatgatgta ctaaaagaaa ctataacaac agatttaact
     2281 cttgaaaaaa caattaacta tacattagaa gataatgctc tacacacact taaaatagtc
     2341 gttacagaca gtaataatgc tacagcagaa aaagtattaa gtataagtaa gaatataatg
     2401 ccattgcctg gagaaaatgt aaatttaaat gatatatcaa caaaattagt tgaggttaat
     2461 gcaggattta aagttgggaa aacaagcatt ataaacactt tagcattaaa aaatatagaa
     2521 gcaagtttga ataacacact ggtggaatta tctgagaaaa taaaaacaag ttttgatagt
     2581 tcagacgcta gtgtacaaga tttgatgaat cagttaacac gagctaataa tactataagt
     2641 cagttaaata caagatataa agttgcgagt ggaattactt atcaactcaa taacccaagt
     2701 ttatctgcca atttttataa tggtggttat acaactactc aagaccattg gattaatgtt
     2761 agcaatcttg gttttgttcc tcatattttt atagctgagt gcgattttac aaaagatggt
     2821 tatttaacta agagtttagt ttttgccagt tataatgttt ttagtaaaga ttatgtaatt
     2881 tcttcatatt ttagacgtca aacaaattca actttttact cacatggcaa catttataat
     2941 cttaatgaaa aagatgttta tgttaatggc agaggtgttc aacttcctgc ttttaataat
     3001 tacgattttg catataagtg gcaagctata aaatttgtat aaatggggtg ataaaatgaa
     3061 tagagcaaat agaataattt acgaccaaac aggcaaaatg cttctccaaa caggagaagc
     3121 aacaggggat gtattggagt atgatacaat aacagaattg cattgcattg atattccgta
     3181 tggaagtata gattatacta aaaatagaat tataggtata aatatagaaa caaaagaacc
     3241 aattttggaa gaaataccaa tattcgttac agaagaagaa aagagaatac aagagttaga
     3301 aaaccaatta ttactaaatg aaaatgaaaa agtaggagga ttattataat gaatataaat
     3361 aatgttgtag taagaatatt agcagagagg attttaagta gaggcttaaa tcctctaaaa
     3421 aatcgagaat ttgaattaga tgatgtaact aatgtagaat acagaaaagc tgtagaggat
     3481 tatataatta gagaaagtgg agtagtcgaa gaagcagaac caactatata gagggttctt
     3541 ttttattgaa agaaggtgac taaatgactt ttaaagagtt agttaataaa gttagaaatc
     3601 ttgtattaga agcaaagaat gtaactatag aagatacaga aaacttattt gaaagtgata
     3661 atgttgaggg agcattgaaa gaattggcaa cagaggtaaa tggacagaga gcaaaaggaa
     3721 ttacaatagc aaatgattta atagatatga tataagcgag gtgaaaatat gacggaaaaa
     3781 ttaactgata atgctagttt aagagaactt atgacaacac tacaaggtgt acaaactgat
     3841 tttcagaatg gcaaaagtaa tataacaact gtgttgggga gcccattttt aaacacagat
     3901 aaattagata taactaaaac aaaaatagaa acattaaaaa gtgcattagt gaataattta
     3961 atatataaag gagtgtcagc aagtacacaa agttcattta ctgatttaat taatgcgata
     4021 aattttatag tgcagtcaat attaattgtt aaatgcaagg gtacaggaag aatagaagaa
     4081 gtaaacaatc cgtacgttat ttataatgac aaacctaata tcaaaggttg tttaaggatt
     4141 aaaggaactt tagaagttta taaaaattgg gctacttttg caagtgctag actagaaata
     4201 atatatggag aaaaaaaaga atatatttat ctttcatgtg atgctactgc tagtggtcgt
     4261 tctacgaatt ttgaaaaaga tataattgta aataatgata cacaattaaa aatacaagtg
     4321 ttattaacag atgttggaac ggggaatagt tctactgcaa aagcttacac atcagtttct
     4381 gacgttacac tgttaagata ggagattagg tatgaaaaaa caattttatt acgattcttt
     4441 agaggaaaaa gaaaaaataa ttaatgaaaa taacaattta tatgtaatag agttatcaga
     4501 aacattaaaa ggtaaatata taattttgtc agataatcca atatacgaaa aacttagtta
     4561 tgaagaatta gaaaatgagt tgttaacaaa cgaattaaaa ggcgaaattt tataacagta
     4621 actatttgtt gagaaatggt tactaaaaag tcttatactt gtttattttg cttctttttc
     4681 tatttaaaat aaatttatct atactatata tatcttgtaa aaattgttta aattcaatca
     4741 cttttctatt ttataaagta accatttctc aacaaatagt tactaaaaat agaaaggtga
     4801 tattttttat gatagaaaaa ttaaacgaaa atgcaagttt aagtgactta ataaccactt
     4861 ttgagaacgt gaaagatgaa ttgcaaattg gtaaaaataa tatagctggt acattaggaa
     4921 gtccttttgt tggaactgat aagctaagtg taactaaaac aaaaatacag accttgaaaa
     4981 atacactagc aattagtttg tctatgaaaa atatatctgc tcaatcaact gaaacacttc
     5041 attcactaat tggtaaagta gctggaatcg caaatatttt tattgcacaa ggagaatgta
     5101 ctcaagttgg agaaaaaact tttcaactct atcaagtaga taataatacg ccaaaaactt
     5161 gttcagcaaa cgcaataagt tttaacttag cttttactcc aaaatttatt attgtctata
     5221 gaaatgctta caatataggt ttgtgtagtt ttgtttttta ctcatctttt cgtaatatta
     5281 taacagcagt gcctcaacct agcaattttg gtgaaatgta catgcctaac acattcgatt
     5341 gtgtagtagg tcacacttgt attttaccag tgaaagatta caaaatacca cattttttct
     5401 atgcaattgc ttaaaaaata agttaaagga ggaaactatg actgaatata tagaagtagg
     5461 aagaagaata ttttttgatg aagaaggcga aataatattc tatgaaggac aatcgaaagg
     5521 aaatgttcca gagcgaaaaa atattaaaaa aatagaatat atagatttgg aatacgatta
     5581 tattgattat gataagtaca aaataaaagg tataaatata gagactaaac agccaatttt
     5641 agaagaaata ccaatctata tgagtgaaga agaaaagaga atacaagaat tagaaaatca
     5701 aatattgatt gcagaaaatg aaaaagtagg aggaatttta taatgaatat aaataatatt
     5761 gtagtaagaa tattagcaga aagaatttta aacaaaggac tgaacccttt gaaaaacaga
     5821 ccttttgagt tagatgacgt gactaacata gagtacagaa aagcagtaga tgattatata
     5881 aatacaatag aacagaaaat aaaatagtaa cttttagtaa ctcttcttaa aggataggtt
     5941 acttttaaag tagaaagatg attaaatttg aacgtttctt ttaatgtaga atttagaatg
     6001 atttctttat aagtatgtga aagtaagtaa aataaacaaa attaaaattt tttagtgacc
     6061 tattctttaa aaatggttac taagaaagta ggaaggtgat atttttatga tagaaaaatt
     6121 aaacgcaaat gcaagtttaa gtgacttaat aaccgctttt gaaaatagta caaatgagat
     6181 taaaacaatt tcaaatacat taaagcaaac tctaataaat aaaaatataa aagtattaga
     6241 tacggacaaa ttaagtagtt tagtcgataa agtagataag ttaaaagcgc aagaatttgg
     6301 tgtaagaata aataagttgg atgaaaatcc agatacttgt gtaagttatc taggagatgc
     6361 aataggaatg actcctgcta gtgttggtag ttatggtagt tgggatacta tagattttat
     6421 tagaaacata aagccatgtg gatttaaaga tggaatagta acaaagtata ttaaaaaaga
     6481 aaattttaat atgtatgaag atggaactaa tgtagaagat gacacagatg ttatgattga
     6541 atttcctaaa ttttattgga aaatacagag ttctgataac tacatggata tatttatatc
     6601 taaaacaaaa ttagatgatg attatgaatg tccagcacat ctaattggta gtacagaaaa
     6661 agaatttatt tatataggtg catatttagc atcattggaa aataataact taagaagtaa
     6721 aaaaatagta gcccagagct gagagaacgt ggacgattct acagtttaat atataaacat
     6781 gttcctggat atgattcttt ggattatcaa tgtgtattaa tgttacagat tctatttgtt
     6841 gtaatgttta aatcaataaa cttttttagg ttaggagttg gttatagctc ttatatatca
     6901 ggtcatggaa agccagataa aataaacaca ggcggttcta atttcaaagg tatgatatat
     6961 ggagaacaaa atggagatga gcaaatgaaa tttttaggaa tagaggattt atggggaaat
     7021 tgtctacagg aggtggggag atgcacttgt ggagttgaaa atgcttgtgt atatacaaga
     7081 ataattacaa gggataaaag aaatatacaa tatggagtag gaagaaaggt tggtacaata
     7141 tcagatgtaa taggtggtaa caacactggt tttatggtta aacagtgtct tcctggagga
     7201 ggaaacggtc aagatagtcg atattataga tgctgggcta atttaagtga aaaccatata
     7261 ggatattaca cagggtttga ttataatgaa aattttagat attatggttt attttcatct
     7321 tattttcaaa cagaggtaaa aaaagcatca actagattaa tttttatagg agaagacaat
     7381 caaaatacac taaaaacaat tcaagaagat gaattgctgt ttgtggaaaa tgaaaaagtt
     7441 ggaggaatat tgtaatgata gatggagtag ttgtaagaat actggcagat agaatattaa
     7501 ataagggatt aaatcctcta aaaaatagag tatttgaact tgatgatgtt actaacacag
     7561 aatataggat agcagttgaa aactacatta tagaacatag tgaggtagta gaaggagcag
     7621 aacttacaaa ataggtaatg ttcttttttt aatacaaagc aataggaggt tttcatgaat
     7681 gaagaacttg tgaaagaaaa aataagtaca cttgaaacaa gagtaaaaga acacggcaaa
     7741 caaattgata gaatcgagat tgagcaagca aagtttgcta tacaaataca aaacctatgt
     7801 agtgatataa aaaatctgac aggagtactc aagtggcttg taggagttat aattacaact
     7861 ttagtagggt tctttatatt tgccatacaa aaaggaatat tttaattaat taggaggtta
     7921 aaagatggat aatttaataa gttttatacc agagcagtta ctaattttag tagctgctct
     7981 ttatgttata ggagcaggtt gcaagaaata taagcaatta gataataaat atattccagt
     8041 agtgctattg atacttggaa taggtttctc aatatggatg ctaggattaa atcctgttgc
     8101 agtcttacaa ggtgtaattt gttggggagt tgcaataggt ataaatcaaa cttacaaaca
     8161 gtttaaggat ggtgaaaagt aatgaaatta acaaaaatat tattactcac aaaaatttaa
     8221 a
//
LOCUS       DAKBNN010000169         8200 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000169 DAKBNN010000000
VERSION     DAKBNN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.170"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(16..1038)
                     /locus_tag="OUB02_003047"
     CDS             complement(16..1038)
                     /locus_tag="OUB02_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCT8886783.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(1038..3458)
                     /locus_tag="OUB02_003048"
     CDS             complement(1038..3458)
                     /locus_tag="OUB02_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCT8886784.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSNKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLSAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPKSLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVYFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKALKENP
                     KSITGRYL"
     gene            complement(3544..4464)
                     /locus_tag="OUB02_003049"
     CDS             complement(3544..4464)
                     /locus_tag="OUB02_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8886785.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            5054..5980
                     /locus_tag="OUB02_003050"
     CDS             5054..5980
                     /locus_tag="OUB02_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HCT8886786.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKVASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(6340..7992)
                     /locus_tag="OUB02_003051"
     CDS             complement(6340..7992)
                     /locus_tag="OUB02_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HCT8886787.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
BASE COUNT         2775 a         1212 c         1097 g         3116 t
ORIGIN      
        1 tctatgaaag agtaattaat ctattataga agtaagtata tatttttcaa taaactccca
       61 tctatcacga ggtttaaaca ctattttaga tggtatggct attactaagt catattgtgg
      121 aatacaacaa atagcatttc ctccagttcc cattgctgaa taccacaaaa caccatcttt
      181 tttacatagc caccacagat aaccgtattc attactattc attttaactg attcttcaat
      241 ccacgattta gatataatac gtttattatt ccaaatacca tcatttaaat acagaaaacc
      301 aaatcgcaac atatctctag cactcaatgt aagtccccag ccacctatag aataaccctg
      361 tctatctttt gcccaaccct taagattttt tccaaagaaa tcttcaaaac caaaatcttt
      421 catatcataa tctggtataa ctttcatacc aataggttta aataagtatt cattagcaaa
      481 ttcacgagca gattttccag tagtttttgt tataatagct gaaagtaggt gcgttccagc
      541 tgaagaatat ttgaaagtac caatttcgcc tttttgacca agttgattca aggtatattt
      601 tatccaatct ttttgtttgc aaagtctttc taaagactca gtcatatttt taaaagggta
      661 tggtgcagtc attgtaagta ggtgacgtat agttactgct tttttacata tttcagaagg
      721 actacaagta tattcaggaa aaaaatcaac aaccttttgg tcaacatctt ttatatatcc
      781 tttatctatt gcaataccta ttaaggctga tataatactt tttgtaactg atgcaacatg
      841 aaaagtatca tcagcattat atccattata gtacttttca aagacaattt ttccttttct
      901 tgcaataatt ataccattta tattgctata ttgtgagatt attaaaggtt caagttgtga
      961 aagcttttct gaactcatac caacacttga aggatctact ttttcaaaaa ttaagtctaa
     1021 gcaattgttt ttattcatta aagatacctc cctgttatag attttggatt ttcttttaga
     1081 gcttttggag aaccttctgc aattatataa cctcctttgt caccaccttc tggaccaagc
     1141 tcaacaatcc agtcacaaac atttaataca tctatatcat gttcaacaac aattacagaa
     1201 tttccatttt caaccaattc atctaataat tgaatcaact tagaagtatc atatagactt
     1261 agaccagtag ttggctcatc caatacataa aggatattac catttcgttt tttaccaatt
     1321 tcttttgcaa gtttgattct ttgggattca ccaccactta aagttggtgt aggttggcct
     1381 aattttatat accccatacc tatttgttct aatacattta agtgtgaaac aatatttgga
     1441 atatccttga agaaatatac agattcagaa acactcatat ttaaaatatc atgtatattt
     1501 ttatttttat atttaataga taaagattca tcattaaatc tttttccctg acattcactg
     1561 catatatggt ctatacttag attatttccc aaccagacct tttcatatcc actaccacta
     1621 catttaggac aagcaccttt ggagttaaat gagaaatgac cagctgtaaa tcctcgttct
     1681 aacgattttg gctgacctgc aaatagtgta cgtatcttat cccatatacc aatataagaa
     1741 gcaggagttg agttcatatt tttgccaata ggagattggg atatctctga atatccagaa
     1801 atatgtttag taccaatcag tctatctgca atagtttcaa caactgcata atcgtcttcc
     1861 tcacctattt cactctctga agaatttatt tgattattga tagtatggtt attaaattgg
     1921 cttctaagaa gaggaagtag ggtgtctgat ataagtgaac tcttgccact acctgatttt
     1981 ccagatattc caaccatagc actcaaaggt aatgatacag ttacattttt aagattattt
     2041 gtatttgcat tttgaatagt tatacaaggt attttatcag tatgtgtaat atttttattg
     2101 cttgttctat taggcattgg atattttcca gatagatact gcccagtaag tgatttgttg
     2161 gattgaagta gaccttctaa atctccttga tatactaatt gaccaccttc aacaccagct
     2221 tttggaccaa tatcaataat atgttctgcc attttaattg tgtttttatc atgctcaact
     2281 actatgaccg tattgcctaa atctttaagt ttctttattg attttaagat gtctattttt
     2341 tctgattcat gcaatccagc tgttggttca tcaagcacat aaattaatga gtccatctct
     2401 gaatcgagat gtgaatttaa aaatagtctt tgcatttccc caccactcaa agttgacatt
     2461 tctcggtata aggatagatg acctaatcgt gattctatta aatgttgagt tttggataaa
     2521 atatcattta caagatttgt accaaatgtg tttaaattac cttgatttat taagagttta
     2581 aggaaatcat caatttctaa aatcgtcatt tttccaagtt ctccaatatg ttttccgttt
     2641 aaaagtactc tacgtgcttc ttcaccaatt ttaaaaccat tacaatctga acaggcagtc
     2701 atagtataaa ctccatttaa atcttcgccc cttttgtaac gattgtgaaa tacctttgat
     2761 aaagaggaac tttttttacc attttcataa tgaccataga gtacttcatt tcttatttct
     2821 tcaggtagtt gtaagaattg tatgtctaaa taatcgttga attttttaga aaatactctc
     2881 aaatatcctg gtgtaatctt tgctctatct aagacttgtt ttagggtgtc atgcttatca
     2941 aggactaatt tttcaatatt aatctcaaaa tatgcaccat gtccagagca cttcatacac
     3001 attccatctg aagagttgta tgagaaataa cttggtctta atcgttcttc ctcatatcca
     3061 caattattgc aaattagatt atcgcttaca ggagtatcac acatagtaca cattatttga
     3121 ccttccactg aatacaatag tgtaagcatg ttgagaatat tggttcttgt gcctactgtt
     3181 gaacgaggat tgctctggcg aataatattt tgttgaacag ctatagtggg agctaaacct
     3241 tgaatattat caaacttatc ttcactatca atacctgcaa gtatacctaa agattgcaga
     3301 tattgtttac gcccttcctc aaatacaatg tcaaacataa ggctagactt acctgagcca
     3361 ctgacacctg ttgcaacaac aagcttatct ttggggatag aaacatctat atttttaaga
     3421 ttgtgtatct ttgctccatt aatttttatt tctttcattg gttcacctca ttttatcaat
     3481 tttatcaaac cgtaaaatag ggatatatta atccttaata caagaataga ttaggtgagg
     3541 ttattaaaaa ggttctatag gtaaatatat atcgaccaag tgtctatttt gagggtgatt
     3601 gtgtgggttg ttaagatata cttcaaaagg gaaggaggca cgtagcttat aattaccatt
     3661 tgatagccat tcattataca taaaatccca cgcatcacta tattcagatt ttgaaatatc
     3721 aaaatgtccc acaagatatt ttccagaagg tattatcata ttgccaagat tgtcattctc
     3781 tttgacaact atgttatctg gaatagtcat gcaaatacta gtccttaatt gatgctcctg
     3841 tgtaaattgt ggattatcat gataaatggc taatatttgt gtgttttcaa gtccaagtag
     3901 attctgctca cttgcatatg tgaataaccg ttctaaaagt tttgggaaag tactggttag
     3961 attaccatat gaaccagtat acctagtgta aatagtattc atattatcaa gttctagaat
     4021 ttcaatttca cctttaacct ctctagtatt cttacattta ggttttgata tgcttctatt
     4081 gtactgagaa attttatatg gttctttgca attcttgcta taattgtttc tatattttat
     4141 agggcttatt ttatagtaat ttttaaatgc tctcgagaaa actgctgaat cagtaaagcc
     4201 aaagtagtgt gcaatatcag taacagtcat gtcagttcta tgaataagaa aagcagttgc
     4261 attttctagt tttagcctat ttacatactg tgccaaagtt tctttttcta ttgctttgaa
     4321 gattctatga aaatgaaatt ttgaaaaacc tgctatatta gaaagttcat caagagaaag
     4381 aggttcatgt aaatgttctt ctatatagtc ttgcactttg caaatacgat ttagatattc
     4441 ttttttactt ttagcatttg tcatatatag ccctccttat tgtgtaaaat gtgtatcaat
     4501 taatttatag caatatagta ataaaatttt atgtttttgt aatttatgat aaataagata
     4561 gcgtttaatt ttttataatt gataaaagat ataagatgcc ttatatttgt ctttgcatat
     4621 aagtatagtc ttcgtaataa ttagtatacc atagtgattt atttttaatt gtgtatttat
     4681 ataaatacac aacttatgat atggacgaat tattatgcaa ataagctgta attaaataaa
     4741 ttgcaatata aaccagttga aacataagtt agggaaatat tatcaaagtt attatttagc
     4801 ataagagtat agagggaata aaataagatt aaaaaattac atactaaatt taataaaatt
     4861 tttatattaa aaataatatt aagtttattg tttaaataaa aaagttaaat ctacactatt
     4921 aacttattat acttaaaact ttaaaatgaa aatatcaaag aatacttttt aataaaaata
     4981 atcatttaat aagttattta aaaatctaaa aactttacaa caaattatct atttaaattg
     5041 gaggagtggg aatatgagta atagttttta tcaattaaat tcagcagtat tatattttaa
     5101 tgaaaaaaga gatgtaaata gaataaggtt taatcactat gtaatgagaa aagaaaatat
     5161 acaaagatta aaaggaactc tgccaagagg acaatttatg atgacagtaa ggaaagttgc
     5221 atctgacctt gatgtatcta tatctacaat aagtagactt gtaaatgaat ttatagattt
     5281 aggtatattg aggttaataa gtaggggagt aaagggaaat tgttgttctg tatatagtta
     5341 tgtttgttct gaaagtagta atgcagaaat tgtaacaaag aatggtgata ttttcaaaaa
     5401 tatagagaaa tacattgatg caactattaa caataaccaa aaaacaatga aatataaaca
     5461 tagagatgag ttgtttaaga acagtaatac caacatatac agccgtataa atcaagagag
     5521 tatagacaaa gattatgatt tgataattca tactgggtat gaaacagaaa atgagacaaa
     5581 gaaaaaagaa ttattaaaaa ataatttaaa taaaaagaat ttaaataaaa atacctttga
     5641 aaattcggat gaagatttga ctgaagattc tacttataaa aatccagaaa gcatacatga
     5701 aattattata agaaaattaa atagtgaatc aggaaaaaat tttaaagctg atttgagtat
     5761 aactaaggca cttatagata aaagattaaa agaagggtat agtttagaag atttttacaa
     5821 ggttatagag gcaaaggtaa gaagatggag atatacagat atggaaatat atatagaacc
     5881 aacaattctg tttgggtatg aatttgagtc gtatttaaat gaggagagtt ctttatctgc
     5941 ttgtaattat ggtgatatgt gttatgaaga tatgtattga gatatttatt aagatattag
     6001 ttgcttttta gttgtttaaa atatgtttat gtataaatag tgtattaaaa tggagttgtc
     6061 tcaaaataga gagaagcctc ttcttaattg tttaaatgat gaaaatatat ttgatttaat
     6121 acaaaggaga gtgctttatg agttagaaga tttattttga gacactcact ttttttaata
     6181 tactggagaa atataaccca ttttacaaga gtgcataggt atatataatg gcataatgtg
     6241 gatttaaatt aaaaaaaata aaataaagcc ataaatattg aaattttcaa tatttatggc
     6301 tttattgctt gtaataatta atagaatata tattttttat taatcatcta tttcaaattc
     6361 taatatttta ccagtaattg cattaattgt tatctcatat tcagtttttc cttttattat
     6421 ttcaccttca tattctttgt catcttcatc atacttaaat tttactattt tcccaccagg
     6481 cactttatct agcattattg atttagcttt ttcagcacta ataatagaac tgttgtcatt
     6541 agtattagag ttattcttat cattatcatc aaagtcatca tcagaatcat tgtccatgtc
     6601 aatatctaca attcttccat caaatgcact tatctctaat tcatatgttt tcttattttt
     6661 tattacttta gcctcatatt cgttatcttc agcatcatat ttacaataga ttaaattacc
     6721 attaggaata gcatctaatg ctatagactt agctttatcc aatgatatat tttgtgtacc
     6781 taaattatta tcatttaagt catcatcttt ttcaatttct attattttac cagagtaggc
     6841 atcaatttct atctcatata ggatattatc ctttataact ttgcagtcat attcattatc
     6901 atcttcatca taataacaaa ctaaaatatt gcttcctgga agagcttgtt ttaatatttc
     6961 tttagcctgt attaaagata tttttttagt tttatttctt atgttcacat tttttagttt
     7021 taaaatatct tcaaatgctt tttgattata tcctcctcct acttgtgtta tttcatctat
     7081 atctttattt ttaataaatt ctttttgact atttacaagg ctaccattag atatcattat
     7141 aggagaattt tctttagcag ctagtactcc tattgataat gaatctacta atgcattttc
     7201 ttggttaata ccatctttag ctacaaatac attatttatt tttttatctt tataaaattt
     7261 atcaataatt tttgcattag tattgtttct atctgaacca ctgattcttt ctctgtttgg
     7321 atatttcttt actgtattgt ctgataaaat cttatttcct ccaactatgt aagatttttt
     7381 tatagaccta ttgtttaaga aatttatact actatcattg ctgttttcat ctgatactaa
     7441 tattataggc atattatgta ttgctgctgg agaagctata ctaactgcat cagatagtcc
     7501 ctttagtcca tttacaactg caacattttc tacctttttt atcttatcta tttctttaag
     7561 tacatttgta gacatttcat atatattgtt tcctactatt ttatttattt ttatgttaag
     7621 tgattttatt tcattttcta ctttggaaga aatgctattt ccactatcta caatgtacac
     7681 attatctaca ccaagccttt taagctcagc cttagttgaa ttttcaatac tgttgttgtt
     7741 tactaaaagt ataggtgcat tctttagctt agcaaatgga tttgcacaaa gtgcactttg
     7801 aatagaatta ccattaacta taatggctgt ttttgattga acccatccca atttacttac
     7861 ttctactgct gtttcatatc ttgttttacc tattaatctt tcttgctttg tagtattgtc
     7921 tgcaaaaatt gctgaagaac tagaaaaaaa catcatacct gatattaata tagctaaatt
     7981 tttcttattc atatttttac ctcacaatct ttaaaaattt gttatttagc atacttaata
     8041 aaatataatc ttaaaatcaa attgatgctg agtttgaagt tacaaaatca acaaataaga
     8101 tttctttaat acaaaatttt cctttttata tttaacattg tatcacaaat ataaaaagga
     8161 aaaaactttt taaaaagggt atacactatt cattttttat
//
LOCUS       DAKBNN010000170         8029 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000170 DAKBNN010000000
VERSION     DAKBNN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.171"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            44..1840
                     /locus_tag="OUB02_003052"
     CDS             44..1840
                     /locus_tag="OUB02_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HCT8886788.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(1980..3761)
                     /locus_tag="OUB02_003053"
     CDS             complement(1980..3761)
                     /locus_tag="OUB02_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HCT8886789.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(3784..5670)
                     /locus_tag="OUB02_003054"
     CDS             complement(3784..5670)
                     /locus_tag="OUB02_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCT8886790.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(5687..6184)
                     /locus_tag="OUB02_003055"
     CDS             complement(5687..6184)
                     /locus_tag="OUB02_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HCT8886791.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(6502..7983)
                     /gene="cls"
                     /locus_tag="OUB02_003056"
     CDS             complement(6502..7983)
                     /gene="cls"
                     /locus_tag="OUB02_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCT8886792.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         2776 a         1349 c         1140 g         2764 t
ORIGIN      
        1 tatatttgag tttcattata taacaagaaa gttggaattt tttatgggaa tagaatattt
       61 tagtaaactt ttagaaagta gtactgaaaa agaattgctc ttaagtaaaa agtcaaataa
      121 cataagtaca gcaagattaa tatctttttt gatagtgata gcagggtttg ctattggatt
      181 ttacaataaa aatatagtag gtatttttgt tggagtttta tcaataataa tatttatagc
      241 actcttagtt attcatagta aagtaaaaga agaagaaaca tattttaaaa gtaagagcga
      301 agtattaaat aagtatatta agagatttgg agatggttgg aaggaattta aaatagatgg
      361 taaagaatac ttaaaagatg aaaattcgca agcaaaagat ttagacttat ttggaagagc
      421 atcattgtat caatatatct gtgtagctaa tacatcttat gggaaaaatt ttttagccaa
      481 atatttgtgg aatgaaaatc cagatgagaa tgtaatctta gaaagacaga gagctataaa
      541 agaactttta tcaaaagatg atttttctat tcatattcaa acactcagta atataattgg
      601 taaagagcaa aaaaacaatt cagatagtag tatagaatcg tttatagagt atggagaaaa
      661 taagaatgtg tatataccaa aatggatgca tatttttact tggggtttac caactgcaac
      721 aatcttatct tttatatttt gtatgttagg atttttacct gttttacctg tttttttact
      781 ttttataatc cagttagggt ttagtggttt tggatatcca agacttatgc aaactcttgc
      841 tccattattt tcatttagta gaagtataca agtatatgaa aaaatgtttg aggttttaga
      901 aaaagagacg tttgaaagtg catatttgaa agaattacaa gataaacttt ctaagggaag
      961 tggagtttca agaggtgtta aacaattaaa ttcaatagga aatgctgtaa acctaaggta
     1021 taatcagata ctatatataa ttgcctgtgg tgttttgatg tggaactatc attgtgcaga
     1081 agctttggag cgatggaaag gtatatatgg aaatcaaatt agagattggt ttgaatctat
     1141 tggagaattt gaagctctaa taagtttaac agttgtttct catgtaaagg aaaatacatg
     1201 ttttccagta ataaaatatg aagatactcc aagattgaaa gttgaagagg tataccaccc
     1261 attaatagca gaaaaaagtg tcatagcaaa ttcgatacaa ttaaattctc agacttgtat
     1321 tatcacaggt tcaaatatgt ctggaaagac aacattttta agaagtatcg gtgtaaactt
     1381 agtgctggca tatgcaggtg ctccagtatg cgctaagagt tttgatgcga cctgtatggc
     1441 tatatttact tctatgagaa tacaagatga tgtaagtcaa ggaatatcaa ctttctatgc
     1501 tgagattctt agaataaaat caatgattca atatagttta aaagaactgc caatgttggt
     1561 tctagtagat gaaattttta agggaactaa ctctgctgat agaattattg gagcaagtga
     1621 ggcagtaaag aaattatcaa aaccctgggt tataagtatg gtgacaacac atgactttga
     1681 gctatgtgac ctttcaggta gtggagatgt tgaagttgta aactatcatt tctcagagta
     1741 ctatgtagac gataaaattc attttgacta taagataaaa gatggaagat gtaaaactac
     1801 aaatgcaaaa caacttatga gaatggcagg tattctttaa ttaatagata ggttaattaa
     1861 agaataagtc tatttagaaa tataatttaa tttgtgaaat taataaggat tgtcttaaat
     1921 tacgtaaaag tattttaaga caatcctttt ttaagaataa attttatact ttgttatgac
     1981 tacttagctt cttcacaagc acattcttgt ggacactctc catagacaca actcttatca
     2041 aagtaatgag tatgaaggta tttatgagct acatcactat ttggttctcc aaagaagtct
     2101 ttataaatag cttgtaaatc aggattctca tgagatttac gaatagcctt atttctatct
     2161 atttcatata acgcatttgc acgtctatca actatttcag tattaccatg gtgataaggt
     2221 tgaccagcac ctccaacaca tccaccagga catgccataa cttctatgat atggtattta
     2281 cattctccac ttctgacctt atccataatc tttctagcgt ttttaagtgt atttacaata
     2341 cacacattaa ctgtagttcc atcaacatct atacttgctt ccctaattcc tttaagacct
     2401 ctaacattag tgaagtttac attatctaaa gtcttattag ttatcttctc atatgaagta
     2461 cgtagggcag cttctaaaac tccaccactt gctccaaata tagatgcagc acctgtagat
     2521 tttcctagag gattatcaaa atcttggtct tgaagattag gtaaatcgat tgcagcttct
     2581 tttatcatct tagctaactc tctagtagtt atagaaaggt caacatctag tattccagat
     2641 tcacttaatt cttctcttga agcttcatat tttttagcta cacaaggcat tacagataca
     2701 acataaagtt cttcaggatt gattcctaat attttaggtg catagtagtt tttagctata
     2761 gaaccaaaca taccttgagg agacttacag ctagatgcta agtttaactt gtctggataa
     2821 ttgtgctcta agaagtttac ccatgcagga caacaacttg ttaatatagg aagattttca
     2881 cctttttgga ttctttctat aaattcagta gcttcttcca ttatagtaaa atctgcacca
     2941 aagtttgtat caaatacatg ttcaaatcca agtgctttta aagcagctac catttttcct
     3001 gtagagattg accctggttc taatccaaac tcttcaccaa tagcaactct tacagcagga
     3061 gcaacttgca ctactatagt cttttctttt ttatttaaaa catcccaaag tttaggtaca
     3121 ttatctactt ctgttaatgc tccagttgga cagacagata tacattgacc acagaatgta
     3181 cactctgttt caaccatatc agagttaaag aatgtactaa ctaaagtatt aaatccacga
     3241 ttaactccag ataatgctcc tactgtttgt atgtcattac atacagtctc gcatcttcta
     3301 cataatatac acttagaatg gtctttaact aaagatttag tagaagtatc tttacctgca
     3361 aaagatttag aaccttgata tcttattttt cttactccta aatcagcagc tatagtctgt
     3421 aattcacaat ctccattttt ttcacagaca aaacaatctt gtggatggtc tgataacaat
     3481 aattcaacaa tagttctacg agcatttaat gctttagcac tattagtctg aactttcata
     3541 ccttccttta taactgttcc acaagctgga actaatcctc tctctgtctc aaccatacaa
     3601 acacgacaag aagcacaagt gtcaagctta tctatttcat tcatatgtaa atgacaaagg
     3661 ttgtgtattt ttatatttat taatttcgca gcatctaaga tagaagttcc actaggtgct
     3721 gaaacgtgtt tgccattaat agttaaatta actaaactca ttgttattcc tcctccaaat
     3781 aagttatttc ttgattatag cgttaaatgt acaattatct atacaagcac cacattttag
     3841 acacttagaa gtatctattg tatgttgttc cttaacagaa cctgttatac aaccagcagg
     3901 acacactcta gaacattttg tacaaccctt acagtcatct gttataaagt aagaaagtaa
     3961 gttctgacat ttaccagcag gacatttctt atctacaaca tgagctatat actcatcttt
     4021 aaaatattga agagtactta aaacagggtt tggagcagct ttaccaagac cacataatga
     4081 agcagtttgt atagtttcag caaggttttc taggtcatct aaatcttgta gagtaccatt
     4141 accttctgta attttagtta atatttctaa taatcttgta gtacctatac gacaaggtgt
     4201 acatttacca caagattctt caacagtaaa ctcaaggaag aacttggcta tatctaccat
     4261 acagtcagat tcatcaagaa caagcatacc accagagccc atcattgaac cgatagaact
     4321 taatgaacca aagtctatag gagtgtctaa atgttcagtt ggtatacatc caccagatgg
     4381 tccacctgtt tgaacagcct taaatttctt accttcaggg attcccccac caatttcaaa
     4441 tacaatatct cttaaagtag tacccattgg aacttcaaca agtcctacat tttctacttt
     4501 tccaccaaga gcaaagactt tagtaccact agaatcttca gtacctaagt tggcaaacca
     4561 atctccaccc ttaagtataa ttgcaggtat gttagcaaaa gtctcaacgt tatttaagca
     4621 agtaggagat ttccatagac cactcttaga agaactataa gttttcattc taggttcacc
     4681 acgtctacct tcaattgaat gcattagcgc tgtaccttca ccacaaacga aagcacctgc
     4741 accatatttt aattctaatt taaagttaaa tccagtgcct aagatatttt cgccaagtaa
     4801 accgtatttt tcagcttgag ctatggctac ttttaatctt tctatagatt ttggatattc
     4861 tgctcttatg tatatgtatc ctgtatcaga acctatagcg tatccacata ttgccatagc
     4921 ttctagtaca ctatgagggt caccttcaag tacagatcta tccataaatg cacctgggtc
     4981 accttcatca gcattacata caacaaactt tttatctgtt ggaccagctg gattttttga
     5041 agcagcttcc catttagagc cagtagggaa acctgcacca cctctaccac gaaggccaga
     5101 agtttttacc tctgcaataa cttcttgagg agtaagagaa gttaaacatt tgcctagagc
     5161 taaataacca tcattggcaa tatattcttc tatactatct gggtctataa gaccacaatt
     5221 ttttaaagct atacgtaact gttttttgta gaaagacatt tcttcttgtt cttcaacttt
     5281 tttacttaaa gattcttcct catataataa ttcctcgaca actgtatttc taattaagtg
     5341 gctttgaact atcttttcag catcagatgg ctcaactttt acgtagaaaa cgttatcagg
     5401 ataaactttt acaataggac cttgagcaca gaaaccaaaa caacctgtca aacgaacact
     5461 aactttatct tggattcctg atttctttat ttcattttct aattcactaa ctatttctag
     5521 actgtttgaa gatgtacatc cagtatcaca acaaacaagt aattctcttc ttaatacatt
     5581 ttccttagaa taattttttc ttaagctaag attaggtttc agctcatcag ctaaaacttt
     5641 taattcatca aatgaattta ctttacgcat tatttattca cccctcttaa caattcaatt
     5701 ctaattctct ataagtatct aatatttcgc ttacttggtc aggtttcact ttaccataaa
     5761 ctttaccatt tacagtaaca actggagcta aaccacatgc ccccaaacat cttaaacctt
     5821 ctaaagaaaa tttaaaatca ctagtagttt ctccaacttt tatgccaagc tgtttttcaa
     5881 aagcacttaa gattttgtca gcacctttta caaaacatac agttcctaga cagacactga
     5941 ttttatactt acctacaggt tctgtagtaa aataagaata gaatgtaaca accccatata
     6001 cctttgcagg agctactcca agttttctag caacatgaag ttgaacttct tttggaagat
     6061 atccaaatat accttgggct tcatgtagaa cttgtattaa cgcaccttcc tttgttgcaa
     6121 gagagtcaat aaatatatct agttcgtcaa ataattgctt gttgtgtgaa ataaaatcgc
     6181 acataacaaa tccccctttt ctttttaaag ttattatttt tcataaaata attaaccatg
     6241 agttgacccc aacattatgt gggtgaacaa aaccaaaaca ataaagaaag aataatatac
     6301 aacaatttct ttatttataa aaacaatgca aaaaagttac ctattaaaaa taaaaactat
     6361 actagtctat tataatataa aataattcag aatagttaaa aaaaataaaa aaaatgagct
     6421 aaattcaata aaaaaaaaat aatacatata aaaaacttta aaaaataaac caaaatgtaa
     6481 aatttttata aaataaaaga tttataaaac tgctgataat agtcttgaaa ttgattcctt
     6541 gatttttata taagtagacc tagaattata tatatctaat gttatttcct tagaatttaa
     6601 aatatcattt tcaaatataa ttctttgttc caatgctttt tttgaagagt acataaatgc
     6661 attaacttcg aaattaagct caaaacttct gatatccata ttagctgttc ctatagagca
     6721 tatagagtca tctataacta ttgtcttagc atgtaaaaat gcattttgac cataagtata
     6781 gattttagct ccaaacttta aaagttctcc agcatatgaa tatgatgccc agtatacaaa
     6841 gggatggtca ggtttagaag gaatcatcac tctcacatca acaccagata aacaagctat
     6901 ttttaaagta tctataaaag ttgagtcaag tattaaataa ggactctgta tgtatatata
     6961 ctttctagct ttctgaatca ttttaaggta accatattta atctcatcaa gttctgatat
     7021 atctggacca cttgatacta tctgaatccc aatattttca gaaggatgat tagaatttgt
     7081 atcttcttct ataaagtact tttctaaatc taaatcttct ttagttgtat atctccaatc
     7141 aaggatgaat ctcatattta ggtcaattac gcaatcacca gtaagcttag tatgtgtatc
     7201 tctccataga ccaaattttg gatttctacc taaatattca tcaccaacat tatatccacc
     7261 tacaaatcct accttaccat caatgacaac aatttttcgg tgatttctat agtttaagtt
     7321 aaagtttaca attttcaaga atgatgggaa gaagcttcca actttaacac cactttcttt
     7381 aagtctggaa agtgtactat tttttagtgt tctaccacca actgcatcaa ataaaagtct
     7441 tacttcaaca ccttctttag ctttgtctat aagaatgtct attatttcag ttccgatttt
     7501 gtcatctttg aaaatgtaaa attgtatgtt tatatatttt ttagcttttt taagttcttc
     7561 aagaagactg ttaaaaaatt gactagattc agcataaatc cacacatcgt tattattggt
     7621 ataatgagca ttatttgaat tagccaatgc ataaatcata tccttatgtt gatgtatatc
     7681 tgaatcaatt tcagatgtag attggagttt aacctgagta tctagtatat tacttttgat
     7741 tatcttatca tccttttctt tcaatctaaa catattgttt tttgaaatat ttctgccaaa
     7801 agctatataa agtatgaatc caagagcagg aatcaatact aatataagaa gccatgttaa
     7861 agttgtattt atgtttcttt tttctctaaa gataaggttt agtataacca tgaaattaat
     7921 tatatatatt gtagtaactg ctatttcata tattgacatt tcgaaaaatg atatatcaaa
     7981 cataaaattc cctccttcaa atatttatta aaataagtaa ttatatata
//
LOCUS       DAKBNN010000171         8021 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000171 DAKBNN010000000
VERSION     DAKBNN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.172"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..656
                     /locus_tag="OUB02_003057"
     CDS             <1..656
                     /locus_tag="OUB02_003057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886793.1"
                     /translation="ESNINNTRINENMVKKNETVGKDTYLSNIMKQKQELNDRIRDLK
                     YRQEVYAKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEDKNKSTMLDENSTYLN
                     TNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKKEKVSEDLSLVGKTRE
                     ELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIFDKTDEQKKHINVLI"
     gene            834..1724
                     /locus_tag="OUB02_003058"
     CDS             834..1724
                     /locus_tag="OUB02_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886794.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMNSLFILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2125..3081)
                     /locus_tag="OUB02_003059"
     CDS             complement(2125..3081)
                     /locus_tag="OUB02_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCT8886795.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3784..4779
                     /locus_tag="OUB02_003060"
     CDS             3784..4779
                     /locus_tag="OUB02_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8886796.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIVPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4757..5620
                     /locus_tag="OUB02_003061"
     CDS             4757..5620
                     /locus_tag="OUB02_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HCT8886797.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5617..6411
                     /locus_tag="OUB02_003062"
     CDS             5617..6411
                     /locus_tag="OUB02_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HCT8886798.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6696..>8021
                     /locus_tag="OUB02_003063"
     CDS             6696..>8021
                     /locus_tag="OUB02_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8886799.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVETFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTL"
BASE COUNT         2967 a          866 c         1260 g         2928 t
ORIGIN      
        1 atgaaagtaa tataaataat acaagaataa atgaaaatat ggttaagaaa aatgaaactg
       61 taggaaaaga cacttattta tctaatatta tgaaacaaaa gcaagaactt aatgatagaa
      121 ttagagattt aaaatataga caagaggttt atgctaaaaa aataaatgac gcaattaaga
      181 acttatgtaa atcagaaata agagaaacaa ctaataactt ttctaatata gaaataggta
      241 ttaaaaatag cattatagaa gataaaaata aaagtacaat gttagatgaa aattcaactt
      301 atctaaatac aaatgatgaa aaagaatctt taatcactaa agagtctaat gaaaaaattg
      361 aagaagaaat attaaatgat gaaaaattag aagagttaga acaaaaaaag gattataaag
      421 aggattccaa taaaaaagag aaagtatctg aggacttatc tttagtaggt aaaactcgtg
      481 aagagcttga aaatatgctt aaaaatttta taaatttaac acaagaagaa ataatgaaac
      541 ttgagtcgag aatagaaaag ttagataaag atgctgaaga atacaaacaa aattcaaaga
      601 ctaatatatt tgataaaaca gatgaacaaa aaaaacatat aaatgtactg atttaaacaa
      661 tatgttttaa aaaaataagt ttattatata atgaaaatat aaaggtacaa tttcaataca
      721 cttctattaa accaataatt tatgaatcag atttaatttc aatttactaa taaccatata
      781 tttatgagtt aataatttta tgttttaaaa agaattaaat aagggggagt agtatgaaga
      841 agaatatatt aactactatg attaccatga ttattatatt gatgatgagt atgacagtaa
      901 tggcagagcc atatcaagaa gatgaaacat ctagcttgaa aaaacagagt gcaaatgaac
      961 gtaatcagac taatataggc acaatggatg gcagagtgca aacaaatgca ataagtaaag
     1021 gtgctaatta tgaccctaaa tatcctctta aaggaatgtt aacacaactt ggtcttaata
     1081 caacaggtaa agagggatgg gatttactct atggaggaaa tcccaatgga gttgagcttc
     1141 gagcagatgg taaatattat tatataaata aaggtatgga agggctagga ttttatgcgg
     1201 cagcattgtc aggtggctca tatcagagaa taggctttgg aaaacttgac cctatagaag
     1261 taatagccga tggaaattgg atttcttaca aacaagctca agatacactt acagtaatca
     1321 ggaatttttt aaatagtttt gactggagaa atgcatctga gatggaaaga gctaatagag
     1381 ctgcaaaatt ggtaaccgaa gcaaaatatg cagactctaa atattgtaat atagtctatg
     1441 gtaatctggt tgataaacgt ggtgtatgtg gaagttttgc aagtagcttt catttattaa
     1501 caaggttaat gggaatgaat tcactattta tattaaatcc gtcattaaat catgcttgga
     1561 actatataca aattgatggg aaatggtata ggtttgatgg gtctgaaata tcagcttttg
     1621 gtggatcatt agactttgat taccgtaaat taaaggatgc aacaagagag atgactactt
     1681 actatgatgc taaggcattg tctatattag gatttaatca ataaaaatag gggaggcttc
     1741 ttatagagcc tttttttatt gatttgaaat ttaataagga attttatatg aaaatttaat
     1801 caataaaagc aaaaaaggct ctttataaag cctttctaag tgttttttta agtaaaattt
     1861 tttatatcaa catcagtttt aatcaaaaca tataacattt tggacttact tttaaaaata
     1921 aaattattaa aatataatat taatcatgtt aataatgttt ttagtttatt atattctata
     1981 atagtggtca tgttgactat tgtagtttta ttaagaatag ttggtttata taaatatatg
     2041 caaagatagc ttgttgttta ctttacttat tagtatatag gacaagatac tctaataaat
     2101 ttgcctattt taatatcttt tgtactaaaa ccaactaaag tttcctttat tatttcgttt
     2161 tgaataatac aaagcataat ccgattgttt gtataaagac tggaaagtat cagagttatt
     2221 ttctaattta gatataccta tagaacaaga attactctct aggcataatt ctaaaacctt
     2281 ctttgcctta ttttcaacat tctcttttga ttgattacta tattcaaaaa cagcaaattc
     2341 atctccacca agtcttccaa taatagcatc atttacgaat actttagtca aaatatttga
     2401 aaaatgtatt agtatttcat ctcctcgaac atgaccaaag atatcattta cctgtttaaa
     2461 attatctaaa tcaaagatgt aaaagtatcc atgtgaaaca gttttaagca tttcttttat
     2521 gtgtacttca aaaaaatgtc tattgtatag tccagtcata aggtcttttt ctgattttag
     2581 tttaaattca tttgccattg ctagtgcttc ttgtgctttt tcagttattg ttttagggta
     2641 atactcatct ttcatttggt caaagtttgg aatagaatgg tgaacatgga taagtttaat
     2701 catatcatct gtaactctgt ataccatagt aaatcgagta tccatttcta catagatatc
     2761 tttttctagg taatcttttt ctattaccca aagagtacca tatactataa aagcttcgtc
     2821 tgtaactttg gtacatgaat accactcatc tataatctca aagtctattt gctctgattc
     2881 tgtttggtct ttttcaagac ctataataaa ttcttctaat gtttcgtaaa attcatgttt
     2941 tcctgttcca attaatgcaa ggttgtcatc taataaggaa ataataaatt taaggttttc
     3001 ttcttctgga tttaaaatat aaatatgcca aatttttttt gataaatctc ttaattgttt
     3061 ttgtattgta ttattttcca tagattaccc ccttaagatt tacagtttat attttaatgt
     3121 attataagtt gaactcttat tacaatatta ttataatata gaactaaaga aattacatcc
     3181 tgtataggta aaagtatatc ttttacaata aaaatattac agtaaaataa ccagttttaa
     3241 tatacgtatt atatttttaa tttataaacg aatattataa atgaaattta aattcaaccc
     3301 tgaagaaata agctaaatgt attagtatat aaatccatac ttgaacataa ataaccttta
     3361 tgatatgata tcaataataa tattatgaga ggagtgcctt gatgtcggag ttatataatg
     3421 gtaaccaaca attaaatagt tggagacaca tatattctgt ataatcagaa attttaagtg
     3481 tatttttaat acatttaatt tttgtgtctt attctgcctt tatagataac tgacatatga
     3541 caacaaatag tatttaaaat atatattact ttgttaaatt atggtgtagt aggctttaat
     3601 ctgctacact tttattaatt ttgtatagta ttattttgat aactattttg aaatagagag
     3661 tattaatttt tctagtccac aggagttttc tgtttttgtg ctgttaaata taagcacaga
     3721 aaaaggcaga tatatacctg tggacttttt ttatataaat tatgaaatat ggaggtagtt
     3781 aaaatgagta taattgaact taatgaactg actaaatctt atagaagatt tgagaaaaag
     3841 gcaggattta tggggagtgt aaagtcttta tttaatagga aatatacaga aaaaacagct
     3901 atatcgtcat ttgatttaaa gatagaagaa ggagaatttg ttggattaat aggtcaaaat
     3961 ggagctggca aaactactct tattaagatg ttaactggta ttattgtacc gtcttctgga
     4021 gaaatatctg ttatgggtta ttaccctaac aaattagaaa ataattttaa aaaacaatat
     4081 gctgttgtta tgggccaaaa aagtcaacta ttctttgaat tgacaccagc agatacgttt
     4141 cttttattta aagaattata tgatatatct aatgaagaat ataaaaaaaa cttggattac
     4201 tttatcgaat tatttgaggt atctgactat ttaaatgttc aagttaggac gctttcttta
     4261 ggagaaagaa tgaaaatgga actaatagta gcccttctac ataatccaaa aatattattt
     4321 ttggatgaac caactatagg cttagatgct atagcccaaa aacaaattag gtatttccta
     4381 aaagaagtaa atgaaacaaa aggaactact attatactta cttctcatta catggaagat
     4441 attaagagtc tctgtagtag aagtattgtt gtagaaaata gccgtaaaat atatgatggt
     4501 tctacagaga aactatttaa tcattttcaa aaatataaaa aaataacagt tagtttttcc
     4561 aatgaaacta acattgtatt tccaaaagat tgtaaagttg ttgaacaaac aaatcataag
     4621 atatcagtaa ttgtgccaaa agaaagagga aatgaaacga ttaaatggat ttttgataaa
     4681 tatgatgtta aagatgtaat gattgaagaa gaagatatag gtagtattgt agagagaata
     4741 tataagaaag gaagtgatga gtattatgag caactataat gaagcaagtt caaattattc
     4801 aatgttttat aaatttaaac agagtattag aaggtattat gaagtagcca aaattaattt
     4861 taaagtgcaa cttatctgga gatttgatgt ggtggttaat atggtgttta cgattgtaaa
     4921 aattttattt gcttatattt tatggaaagc aatttttggt gaaaataaaa tagtctctgg
     4981 ttttacattt aactctatgt tatcttacta tataataagc tcatttttag caggtattga
     5041 tatgtcaaag agtaccagtg aagagatatg taatcgtgta aaattgggca ccttttcaaa
     5101 gtacatgatt attccaatta atgttcaaaa ttattttatt gctaaaaatt ttggaatgac
     5161 atgtttttat tttgtattta atatgatttc tgcatttgta tggattttta tatttaaaat
     5221 agattttgcc tttacatcaa atccagttca aatattaatt tcaattttga tgatttttct
     5281 agggtttatt tttatgatga aactccactt ctttattggg attttgtcat ttaaatttct
     5341 tgagattagt ttttttagaa tgataattga aaatattgtt gaatttatta caggaacaat
     5401 gataccactt gtactcttac cagaaactat agtaacagtt atgaaggtat ttccatttta
     5461 ttatacaaca tatttgccat ctatgttact aataggtaga aacagcaatg aggcaataca
     5521 aggattaatt attctagcat tatgggtttt atttttttct gtagtaaata aagttatgta
     5581 taaaagaatg agaacattat ttgatggggt gggtatatga tgaaaaaaaa tttgaaaata
     5641 atatttatgt tattaaaaat gaaattatca aagatgatgg tatttagatt tgattttttt
     5701 ggagcatttt ttgtagatag ttcattattt atattacaac ttctaatgtt taatagtata
     5761 tattctcatg tagactcaat agggggatgg caacaaggag agatgttaat atttatagga
     5821 actttctcat taataaatgc gataaatatg acgattttct tttttggtat ttatgatatt
     5881 ccacgaaaaa tccaagaagg agaattagac tactatatta caaaaccatt agatccacta
     5941 tttaggttaa catttgaaaa tattaatatt ggttcatcac cattaattct agcaagtata
     6001 cttattgttt tatatggaat atctgagtta aactcagaga taactttagt tatgataata
     6061 atatatattt tcatggtgtt attgatgaca ttactttttt atgatgtatg tatcatactt
     6121 agaacagttt cattttttgt tatatcatct tctgcgataa tgagattaga agataattta
     6181 ttgccaatga atatgaaaat tcctggtgta atatatgaag gtgtttttaa agtattattc
     6241 tatttaatct taccatatgg aattatgtcg acaattccga ctcaaatttt ggctgggaca
     6301 ataacaatta atggattaat atactcaatt ttaattgtat ttttgtttac attgtttatg
     6361 tggagatttt ggaagttagg tatgagacat tataaaagtg caagtagtta ataaattagt
     6421 aaaaataaac aatgtataac tatatataaa tacgtttcat atgacactaa actatgtgaa
     6481 caagtatttg cgctaaaggc actgaaagct ataaaattat taaagtaaga atttaaaagt
     6541 ttctttagta gaaatataat tgtgtaaaaa agcaagatta attaaaacta tatccaaata
     6601 attagatata attcttatgc agaaaggagg tctgtaaatg tctaattgtt tggaatatat
     6661 tttaaaaata attaatgtac agaaaaataa acaaaatgag tgatgtatat tattcagaaa
     6721 atcatgaaca agataatctt tatgagcaag ataatttact ggcacaaaga tttaatttat
     6781 ttactctatt gaagtttgca tttcctacta tttttatgat gatttttatg gggttgtata
     6841 caatcgtaga tactatattt gtatcaagat ttattaatac aaatgctcta tcagcaatta
     6901 atattgtatg tccaattatt aatcttatag ttggaattgg aactatgctt gccactggag
     6961 gaagtgctat tgtagcacgt aagatgggaa atggtgatat tgagggagca aataaagctt
     7021 tttctttaat cgtattatca ggagttgtaa ttggggtaat aattactggt ataggtattg
     7081 tatttatgga ttatatcatt atatggttag gagcaagtga agttttaatt aaatattgtc
     7141 gttcatatct ttttattatc ttggtttttg cttctgcaaa tatactacaa gtattatttc
     7201 aatgtttatt tgtaacagct ggaaagcctc aatttggatt atggctagtg ttaggtgctg
     7261 ggtgtgctaa tataattctt gactatgtgt tcattgttct gttacaaatg ggtattagtg
     7321 gagcagcact tgcaactgga gtaggatacc taattcctac catttcaggt atggttttct
     7381 tttcaaaaaa taaagggtta cttaaatttt gtatacctag actagataaa agaatattat
     7441 ttgaagcttg ttttaatggt tcatctgaaa tggtgggtca actatcaaca gcagttacta
     7501 cttttttatt taatgctatg atgatgaaat taattggtga agatggggtt gcagctatta
     7561 caataattat ttattctcaa tttcttttaa cttcgcttta tataggtttc tcaatgggtg
     7621 ttgcaccaat aataagttat aactatggaa gtgaaaataa tacgcagtta aatcatgttt
     7681 ttaagatttg tatattattt atttcagtag catctttatt ggtatttatc ttttctatat
     7741 catgttcacc aatcattgta gagacttttt ctcaaagagg aagccgagtt tatgaaattg
     7801 caaggtccgg attttatata ttttctttta gttttatttt ttgtggatat aatattttta
     7861 cctcatctat gtttacagca ttatcaaatg gaaaggtgtc tgcaatatta tcttttctac
     7921 gtacatttgg atttataact ataggtttat tgatattgcc aaatatatta aatataaatg
     7981 ggatatggtt agcagtacca agtgctgaga ttttaacctt a
//
LOCUS       DAKBNN010000172         8016 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000172 DAKBNN010000000
VERSION     DAKBNN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.173"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(489..1307)
                     /locus_tag="OUB02_003064"
     CDS             complement(489..1307)
                     /locus_tag="OUB02_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8886800.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1475..2056
                     /locus_tag="OUB02_003065"
     CDS             1475..2056
                     /locus_tag="OUB02_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8886801.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2053..2247
                     /locus_tag="OUB02_003066"
     CDS             2053..2247
                     /locus_tag="OUB02_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886802.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2411..4153)
                     /locus_tag="OUB02_003067"
     CDS             complement(2411..4153)
                     /locus_tag="OUB02_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HCT8886803.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKIQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            4582..5958
                     /locus_tag="OUB02_003068"
     CDS             4582..5958
                     /locus_tag="OUB02_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCT8886804.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            6269..6940
                     /locus_tag="OUB02_003069"
     CDS             6269..6940
                     /locus_tag="OUB02_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCT8886805.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            7129..7926
                     /locus_tag="OUB02_003070"
     CDS             7129..7926
                     /locus_tag="OUB02_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCT8886806.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRVVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         2971 a          978 c         1227 g         2840 t
ORIGIN      
        1 tatcctaatc aaattctagt catttaaaca taaaatcata attgatttat ctgacaaatt
       61 gagataatat cctaattaaa ttgtaggtat ttaaacataa cattataaat aatttatctg
      121 acaaattaag ataatatcct aatcaaattc tagtcattta aacacaacat cacaagtgat
      181 ttatctgtca aattaagata atatcctaat taaattgtag gtatttaaac ataacatcac
      241 aagtgattta tctgtcaaat taagataata tcctaattaa attctagtca ttcaaacata
      301 acatcacaag tgatttatct gacaaattaa gataatatcc taattaaatt gtagatattt
      361 atacataaca ctaaaagtaa tttgtttaac aaattaagat aatatcctaa tcaaattgta
      421 ggtatttata cataacacta aaagtaattt gtctaacaaa ttaaaatatt atccaattaa
      481 tataaaaatt acttcttttt aactgggaag catacctctg ttacccaatt atcaggatta
      541 ctatcgttgc caggacttac atgataaata ttaaacatag gaccatctac atcatagtta
      601 ttatctgata tccaattacc aatagcctca caagcgtctg caacttgatt gaaatttcca
      661 ttaacaattg cagttgcagc agtcacactt ggaacagtct taaatttaac atgttcagta
      721 tctttatact ttccagatac agcaacttgt acttcaacat ctacataatc ttctttatat
      781 ccagtatcat aaaaaactgc tttagaatac tttgggggtt caatttgaac attctggtct
      841 tttgtctcta aaaatacttg atgccataac attccttcgt cttgataagt tggtataacc
      901 cttctaagtg tcatcatata ttttggtgca aaattcttta tagttacatc ataatttttc
      961 ataatatcat ttcctccaat tcttttaata gtggtttcta tttttaatag ttttttctgt
     1021 gtatcttcta attgttcttt tacttgagaa tggtggatat ttagatattt tatgagactt
     1081 tctttatcgt tatattctgt caaaatttct tttatagaat acaagccaaa gcccatatct
     1141 tttagtgcat ggattctgtt tgtagtagaa agttggtctg cactatagta tctatatcca
     1201 ttagttttat tcgtatgaga aggagttaaa agtcctattt catcataatg acgtaacata
     1261 cgaatactaa ttttacttag ctttgaaaaa tcgcctatct taaacatgtt tttgtctcct
     1321 ttataaaggt acttttagct taatattaat ataaaccata tcataatgtt agagtcaact
     1381 aaaattataa ttaaattata ttgacatatg atgaaaaaaa tattatactg aacatgttca
     1441 gaactgaaaa tgttaattgt tttggaggtc tattatgaaa agtaatgata aagatgctag
     1501 agaacgtata attgaagtta cattgaatct tttaaatgaa gtagatgata ttgaggagat
     1561 tacagttaga aaaattgctg agcgtgcaaa tgttggagtt ggtttaataa attatcattt
     1621 taaaacaaag gataatttgt tgagtactgc cataggagat gtgatgtcaa atataatagc
     1681 agagttatat gacgatagtg tatatactct aagacctatt gaagatttga aaaatttatt
     1741 aaaaaaattg tgtgatactg gattacatta tgaaaaagtg cttccttttg tgttgaatca
     1801 atgtataacc aatggagata tgcaagcaga acttgatatt gtccctatgc ttcgtaaaat
     1861 ttttggaaat aaaaaagatg aaatgagtct aagaatcatt gccctgcaaa ttattttacc
     1921 aatacagatt tctgctttat ctacagaatc atttcagtta tacagtggta taaatataaa
     1981 gaataaatat gagcgagata aattcattga tattttaata gaaaatatta ttggagagga
     2041 tgttgatgta agatgaaaat tataactatt ggttcaagtc ttatcactgt tttacttttt
     2101 ctttcaacta tggtttgtgg gttctggatt aagaataata aggtaacaga tgcaagttct
     2161 attaagttcc atatgaatag tgctattttt acagggatat ttttattgat ttccacaatc
     2221 cttttaatta tatatataaa aaaataataa tattattaat aaataaagta tcagaaatta
     2281 taaagacaag agtcttaaaa agttctgata ctttaccata taattttatt aaacattaat
     2341 tgtgtgtgaa aaaatatcac ttatttcaat ctaatactaa atcttaagaa tgagtataaa
     2401 gtattagaaa ttaagaagca ttttttagtt caagtggatt atctatacca aaataatcat
     2461 ataaagtaga gcttatttcc tctaaatttc cattttggaa aggtaattgt aaatttacct
     2521 tttctaccac atctttaaat ccagcatcag tattttgatt taaaaaatcc atatatacat
     2581 caacagcttt tctgtaatct tcttgtgata attgccatat ctgaagtgat acatcagatg
     2641 caagaccata actcaagtaa taaaaaggaa cttggaagtt atgagataca agtatccaaa
     2701 aaggagcatt tttttctcta agtatattat tggaaactcc atattcttcc tctaaatcaa
     2761 aaaacaattt attgatatca tttagactca tatatggatt cttgtatata atttcttgaa
     2821 attcatcata aagacaagca tcaataatag tatttaaaac aatcgataag tgttcttttt
     2881 ttatagcttc agattgttta ccaaaaaaat catcaaaata tttataaaat agaatttcta
     2941 aagatgttga atgaacttca catacatcta tacttggttg tatcttatta tttaagttaa
     3001 tactgttgta gtaattataa aaatgaccaa actcatgagc aaaactagta acacctaaaa
     3061 aagaattgtc ccatgtatta aacaagaaag gtgctttata tttatttata taagtagtat
     3121 atcctccagg tgatttgttt tttccagaag aataatcgta caagtcatat tttttcatat
     3181 agtcgaagct ctcttttagt tttggagata tatcttcaag tactttatca aacttcctaa
     3241 atgagcgatt tttatatact ttaatataaa tagaagaatc agatggttta gatgaaatct
     3301 ctctatataa tggaactatg taatctttta cgtcttgtct aaatttttta gcttcttctt
     3361 gagaatagtc tttgtttagg ttcatatagg cataatctat ataattctta aatccttgct
     3421 tacttgctat ttcagttctt gtctgtataa gttctaagaa tatgttacca aatataggat
     3481 tgtatttttt taagtaatct gaataaattt tcacatattc ttcagggcta agatttggtc
     3541 ttgacaaggc ttcttcaaag tctacctcta taccatctat tgatactgta gatttagcta
     3601 ataagtcttc atattttaca actaggtctt gctctttttt aagcaaacct tcaacttctt
     3661 tagaattaag ttttccaatg ttctttagat aattaaatgt atcctttcca agttcttttt
     3721 ctaattcttt agagaattta gaagttacaa atctatcttg aaagtctttg tatgacatgt
     3781 caacatctac tgatttactc ataagatact tatattcctt agagtaaaat tcgtctgatg
     3841 aatctatgtt atttctaatt tttgctatag taagtgtact gtaaaaatct tgataaattt
     3901 tatcaacttc attaaataaa tttaattgtt ccttagctgt tttagctgtt aaaagtttgt
     3961 cgttgcactt gttaatgttt tcgcaaattg actgtatatt aggacgctta tattccattt
     4021 tttcaaaagg aatagttcct ctaacagaat ctttttttgt atcattagta taatttctta
     4081 tgtatgatgc aaatataata aaaactgcaa ttaaaattag aaaaacaaat attttcttct
     4141 tggatttaac cataaaaatc ctccaatcat ggatatagtt attaattatt ttactatatt
     4201 aaatataaaa aatgcatgtt aagaaaattt aacataaaga tatagataag attataaata
     4261 atataaaaaa tttaacttca aaaaagtata tgaaatttat actggtaaaa tgataaataa
     4321 attttaagta caaaatagta aaagctatga tagttttaga cctattatat gtaaaaaata
     4381 tagatgtttt tggtgcaatt taaattaaaa gatatatata aaaatttaaa atattccaaa
     4441 cagaaattat aaaaattact atcattgtct ttatacaata tttatactat aatagaataa
     4501 taatttagag gaaagctcta aaaataaaca aattaagggt cttaacagta gtgattttat
     4561 gttaaggagg tataaagaaa gttgaaaaca atagttagaa gacttattct ctacgcagat
     4621 ggaatgcgtc ctactcaaat aatggtatct gggtttgctg cgattattgt aatcggagca
     4681 ttgttgttaa cactcccgat agcgtcgcag agtggagaga gtataggctt attaaatgct
     4741 ctatttacgg ctacttcagc ggtttgtgtt actggtctag ttatggtaga tacagcaact
     4801 tattggagtt tatttggaca gatagttata ataacattga tacaaattgg tggtcttgga
     4861 tttatgaccg tagccacaat gttttcactt atggcacgaa aaaaaataca actaagagaa
     4921 agattattaa tacaagagtc attaaatcaa gctgatttat cgggacttgt aagacttaca
     4981 agatttgtat taataataac cataacaata gagggaatag gtgcactggt tttatcaact
     5041 gtctttattc cacaatttgg tttgtctaaa gggatatggt atagtgtatt tcacgcaata
     5101 tcagcgtttt gtaatgccgg atttgacttg atgggcagtg taagtggccc atttacatcg
     5161 ttaaattctt atgtaaacaa ctttacagta tcaatgactg tttgcgcact tatagtgctt
     5221 ggagggctag gattccctgt tgtacttgat attgtaagaa aaagaagatt ttcaaaatta
     5281 aatgtgcatt ctaaggttgt attgttttca actgctacac ttatttttgt gggtgcatta
     5341 tttatttttc ttatagaatt taatcaaaaa gctactatgg cggacttgcc tttaaaggga
     5401 aaagtactat cagcaatatt tcaatcagta acagcaagaa ctgctggttt taatacgctt
     5461 gatttagcta ctttacgaga aagtagtgta tttgtaatga taattttgat gtttattgga
     5521 gcatcacctg catcaactgg tgggggtata aaaacaacaa cactagctgt tttaatcatt
     5581 acagttagaa gttttctatc aggaaaatca gatatagaag cttttgaaag aagattagct
     5641 ccatcaacta taaaaaaatc tcttggtata tttgtaatca gtatatcagc agttattttt
     5701 ggaacattga ttatttcaat aactcagcct aattttacat tagttcaatc agcttttgag
     5761 gttacatcag cacttgctac agttggttca agtttagctg gaactccaaa cttaaatgct
     5821 ttaggtaaga ttataataat aatctttatg tttatgggta gagttggttc tcttacttta
     5881 tttatggcta tactttctgg tggtagaaga aagagtcaac caatcagata tgcagaaggt
     5941 aaaattatgg ttggttaagt taccaagaag tagtataaat ttataatgga tttagagtag
     6001 cactgtaaat ctgttgataa aataaatgag cctagatgta attatatgaa gaaataaaat
     6061 cgactaatga acaaaattaa ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa
     6121 aaggtaaatc aaaattaaat taatctgtgt atataaaatt atatattgtg tataagtttt
     6181 gtatgtgtaa agtatataag ttttgtatgt gtaaacaatt aatttaagag atattatata
     6241 aattgtgtat tagggaggaa gtttcgagat gaagcaatat atagtcattg ggtgtgggag
     6301 atttggaagt tcagttgcgt ctactatgca tcttttagga catcaagtaa tggcaataga
     6361 caaaaatgaa gattcagttc aaagtatatc tgacaaggta acccattcac ttatagtgga
     6421 tgttactgat gagcaagcgt taaggtcatt aggtttaggt aactttgatg tagcagtagt
     6481 tgcaataggt tctgatataa gggcatctat aatggcgact cttatagcca aagaaatggg
     6541 tgtagagttg ataatatgta aggcaaagga tgaattacaa gctaaagttc tttataaaat
     6601 tggtgcagat agagttgtat ttccagaaag agatatggga gtaagagttg cacacaattt
     6661 agtttcggat aatatattag accatattga acttgaccca gagtattcaa ttgttgaaat
     6721 cgtaactcca aatagttggg ttggcaagac acttatagag cttgaattaa gagctagata
     6781 tgagataact gtacttgcta taaaaacagg taaaaatata aatgttacac cttctccaga
     6841 tgaggaactt acagccggaa gtatcctagt tataatcggt caaaatacta gtataacagc
     6901 gataacatct ggaaataagg ggataattag aagaagataa tttactattt aatatatatt
     6961 taattgcaat gaaagtagat agtatcatat aattatgaat agttatatga tactattttt
     7021 ttaatcgaag gtagtattta tttgtaagat tagataaaga gaaattaaat taaaagtaag
     7081 gaggctgtgc taatataaaa atttataagt tattagcatt tagataaaat gattacaaat
     7141 ataaacagta aggataatga aaagttaaag tatacaagag cactattaaa atcaaagaat
     7201 aggaataaag agtcaaagtt cataatagaa ggatacagag tagtaatgct tgcacttgaa
     7261 tgtatggcaa accttgatta tgtatttatc aatgaagaat ttgaaaataa gaaagaacat
     7321 gtaaaactat tagaagattt ggataaaaaa aacacaaaga tatacaagac tactaataaa
     7381 aactttaaag aattagtgga tacagaaaat actcaaggaa taataggtgt agtttcattt
     7441 aagaaaaaaa aattaagtga aagtataaat aaaaaagata aatttgtatt gattttagac
     7501 agaatacaag acccaggaaa tatggggact ataataagga ctgctgattc tgctggagta
     7561 gatgccataa tagcactaaa gggatgtgtc gatatataca acccaaaagt aattaggtct
     7621 actatgggtt ctatttttga tatgaatata attgatgctt cacaagatga aactgtggac
     7681 atgcttaaat cattggattt taatatagtt tcaagttact taaatacaga aaatttttat
     7741 gacaaaatag attatggctc aaaagtagca ttggtgatag gaaacgaagc aaatggaata
     7801 aatgaagaac ttgtatcaaa gtctgatatt ttggttaaga tacctatata tggtaaagcc
     7861 gagtcgttaa atgctgcgat aagctctgct atactgatgt atgaaataaa aaaatactta
     7921 atttaatgta ttgtaataaa tataatgtca tgttataatc ttgaattaaa tagtagagta
     7981 tcaaaaatag tcaatatata ttaaaaaaat aattaa
//
LOCUS       DAKBNN010000173         8012 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000173 DAKBNN010000000
VERSION     DAKBNN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.174"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(110..742)
                     /locus_tag="OUB02_003071"
     CDS             complement(110..742)
                     /locus_tag="OUB02_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCT8886807.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(814..1167)
                     /locus_tag="OUB02_003072"
     CDS             complement(814..1167)
                     /locus_tag="OUB02_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HCT8886808.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1245..1448)
                     /locus_tag="OUB02_003073"
     CDS             complement(1245..1448)
                     /locus_tag="OUB02_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCT8886809.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1838..2068)
                     /locus_tag="OUB02_003074"
     CDS             complement(1838..2068)
                     /locus_tag="OUB02_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8886810.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2072..2476)
                     /locus_tag="OUB02_003075"
                     /pseudo
     CDS             complement(2072..2476)
                     /locus_tag="OUB02_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(2836..3285)
                     /locus_tag="OUB02_003076"
     CDS             complement(2836..3285)
                     /locus_tag="OUB02_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8886811.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AIKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3299..4225)
                     /locus_tag="OUB02_003077"
     CDS             complement(3299..4225)
                     /locus_tag="OUB02_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886812.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESNLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4350..5132)
                     /locus_tag="OUB02_003078"
     CDS             complement(4350..5132)
                     /locus_tag="OUB02_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HCT8886813.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5288..5908)
                     /locus_tag="OUB02_003079"
     CDS             complement(5288..5908)
                     /locus_tag="OUB02_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT8886814.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5996..6559)
                     /locus_tag="OUB02_003080"
                     /pseudo
     CDS             complement(5996..6559)
                     /locus_tag="OUB02_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6795..7610
                     /locus_tag="OUB02_003081"
     CDS             6795..7610
                     /locus_tag="OUB02_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT8886815.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIGNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PYIFMHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
BASE COUNT         2606 a         1379 c         1170 g         2857 t
ORIGIN      
        1 atttaatgtt gtattaaatt tatatatatt aaaataatat acaaattttg aaaaagctct
       61 tataaaatga ttattaaagt aaagtttaat ataaataaat tataaaatat taaactaatt
      121 tatcaatatt tcctgattga atatgcttaa tgtttgattg aattttttta tatggccaat
      181 cccaccattt aatttctaat aattttgaaa ttgtatcatt attaaatctc ttttttataa
      241 tttttgcagg tactcctcca acaatagaat acggtgggac gtcattagta actactgctc
      301 ttgttccaat aattgcacca tcaccaatat ttacacctga cataattata gaatcataac
      361 ctatccacac atcatttcca attataatgt tacccttatt gtcccaagcg tccgttatat
      421 ggttgatatc gagtttccat tcttcataaa agatgggaaa agtatatgtg gattttgatt
      481 tcattgagtg atttccacta gtcattagaa attttgcatt acatgctatt gagcaaaact
      541 taccaattat taatttatca ttgtttatag ggtaatgata caatacattg tttttctcaa
      601 aatctcttgg gtcattataa aagtcattat atattgtgta atctccaact tctatatttt
      661 cccttgttat tacattttta agatatacag tttgataatc attacttctt ggatatacct
      721 tatttgaact tggaatagtc atatttaaat ctctccttta atatatatta tgtttttgac
      781 tattccacta ctacaataca tctcttcaca gaatcactcc ttgttaagaa tcttcataag
      841 ataatggcgt ccttttttag tgttaagtcg gatattcttt cttagtacat tctttttagc
      901 gtataactga caaactgtca ttttcttacc aatagtgtca atgctatcat caaggtcatt
      961 ttgtattgct ttttccttat cccttaatga tatatcatca cgttttccct ttgggggttt
     1021 atcagtaggt acaagtttcc aagagtaaac aaattgtggt ttaccaaatg aatatacata
     1081 cttatatgcg tatcttccat cttttctctg gctctctcca gttcttaaaa ttctatcttt
     1141 attatctcgt cttttttcag ttgacatgat aagaagctcc tctcaataac cgaattgagc
     1201 ttgatacgtc atatgaatat tataacatat aaggctcaat ttcattagat aacgtcaagt
     1261 gtatcaacga ttttctcaaa ttgttttctc tttatttgaa tgcgattgct attcataatg
     1321 acccagccag aatcaaggtt ttcatcagca agtttacgta attttttctc tccaatacga
     1381 aagtattttg acgcttcttc aatcgtcaag gtatattttt cccaaatagg tacatcagta
     1441 ttgttcatag aatgtatcac tcctttctat gttcgtagtt cagaagtaga tagatggctt
     1501 tggtaagctc cattggtatt tcaaccaatc ccattcttat gggcagaacc gtgtcataca
     1561 ggtgtatcat tatactgtgg tacaggttac ggcaaaacaa ccgttccaaa ggttattcac
     1621 aaatcactat atagtagctc tcttgattaa ggtcatggcg tatagtattt gttggctatc
     1681 tgtatcacaa acgtatctat ctactttatt cagttgtcaa agaacagtcg gcattgattt
     1741 gcctataaaa aatcacaata gcttgtgggc ttttatagac aaataattag tgattctcca
     1801 tgtaccaaat agcttgatac atggagagag ggcgagatta agacaaatta aatctcaaaa
     1861 tagcagtgat tagttttgtt cctagcattc gctttgtttc ttcttctaca tgaatatagg
     1921 gatttccgta ttcatcaaat aaagttttct gtgaaagacg aatgatatag tgttcaaaat
     1981 gtttaagtat tccgtttatt gcatttacat cgccatgtac agcaagtaaa ataattgtaa
     2041 aaggtaatac ttctttagta ggtttcattt catgttcctc catgagttct ttcaactctt
     2101 ttagtgcatt tgttttgtga taatgtatgg tattttctgc taaatccatc aatgttgcta
     2161 tgtcagcatt tgtcatgttg agaaagaatg aaagtagaat tatatcttgt tttctttttg
     2221 taagttttcc gattgcttgt gccattaata catcttcaat tcgtatgttg tagtcgtaag
     2281 aaaccataca gatgtagtta gaatcataat catctaaaat actcaattta cttaattctg
     2341 caagtgacaa agattcgaga gataccaaat aatcatttct gcgtttattt tcgtcataaa
     2401 tatctctagc atagttacgc aaaactgttt tacagaaact gtcgaattga tgttcaattg
     2461 tcttttcatc aaccataaga tttcaccctc tttccatcgc aaggagttaa ataaccttgc
     2521 ttactcctat cacggtggga gggaattaaa tcttcgataa atttcaaaaa aaagagaaaa
     2581 aaatacacac tctgcctata gggcaaaaat gtgtattctt taaaacgtat ttaatttata
     2641 ttggttgtaa gccaccacca tattgatgta tggaaatatg cattggctag cttttcataa
     2701 aaatacaaag atgcataaga aattaataga ggggaaaaac ttattctgtt attaaaaagt
     2761 aggttaattc taataaatgc tattgcggtc gaattaaact atatacatgg caatcaatca
     2821 tgtttctatt ctttattatg aattgtgaca gtattcttca atcttaaaac cacatttttc
     2881 caatacttta cttgaccctc tattgtagga atatacatta gcatgaattt tgttaaggtt
     2941 gtatttttca aaggcaattt tgcatatttc ttttattgca tttgttgcta tccctttgcc
     3001 ccaatagttc ctagcaagcc agtaacctaa ttcagcattt ttattaatag aggtatcttc
     3061 ttcaaatgtt aggctgatac ttccaatagc cctttcttga taaaaaatag catagctaag
     3121 ttgtttaaca tctggagttg ccaaacaaaa actaataaaa tctctagcgt tttgctccct
     3181 ataagggtgg gggaagttgt cacgaagatt gtttgcaatt tctttatcat tcgcaaactc
     3241 aactaactcg gtaatataag cataattcca ctctataagt tccatttttc ttctcctttt
     3301 atgaaacagc tacaagaaat ctttctaagc caataccact tatcaaacag cgttcttttt
     3361 tatttccaag catttgataa gtccagtcaa caaatccacc gtcagctatt tcaacggttt
     3421 catttccttg tttgaccgat attttgaaat tgataccttt ataataatta ttatcaacat
     3481 catcattatg aattgataac tctatattag gaaaaataag tttgatagtt tcaaccattc
     3541 tgtcgaaaaa tccgtcatta tctttgtatc cgtttctttt gcgtagaata atagagaaac
     3601 gaacattttc tatttcactt aatatatctt tataatagtt taagtgcttt tctaacaaca
     3661 ttttttcaca agtgtaagac cctgtatcta taccagatga aaccatacaa tataagccaa
     3721 aatgtgaata taaaccttta cctacaaaat tttgtgctct tattacccta gcagtagtag
     3781 ccatgtggat aggtatggta ttattttctt cttttctttt taatttatca gcaataatta
     3841 ttgccaacat atttgagggg tctgcaagtg tttctgtccc tcgtagagca cttactacgt
     3901 tatattgatc tacggagcca aaaacgctac agctacctaa tggggcagag ggagaaagca
     3961 taatcgtctt gatatccata ttgaatgctt ttctcaataa atttgattcc aaagcatgaa
     4021 tttcttctgg atctataccg ctagggatag taaatctatt tagttggtat gactttagaa
     4081 tatcagttgc agttaaagta tttgcttgtc tatcaaacac ttctaataga agtgagttca
     4141 aatctgattt tgatagagct gataacttat caactatatt cttatcccca attttatttg
     4201 taattctact taaaatatca ctcatagtta tcctccatac tgttactttc aaaataatta
     4261 tagaattgtt acaacaacta atctaggtca tttgaccaaa aatgaaaaaa cactgaatat
     4321 ttactattca atattttttt actttgggtt tatttgatta aatcttgaaa atactgtaat
     4381 gcaacccttt cagcttcaac ctttcggaca ataggacttt gtgataattc ctctcttaac
     4441 tcaagataca ggttgatagt agcctgcaat gcattaacac agcttttata ttcatggtta
     4501 gcaaccgtct tttctaatgc ttcaatatat tggggtgcgt ccaattcaat ttttctaact
     4561 ccacgaggta atttacctaa gagaagttgt attaatgggg caagtactga ttgtcttaaa
     4621 aaagataaaa aatcaattgt ttcaaatagt tctctacgtc caatcttagt gcaagcgtaa
     4681 tgaacccaaa tccaaaatct atcttcaatc cactgtaagt ctggagttgg gaagtatgct
     4741 tcttttttag aataaatttc tgttatgcag ttgttttctt catagagaat agctgaatct
     4801 tcaaccctat ctagtaaacc atctaaggtt gtaaacttaa aatcaacatg aagaacaggg
     4861 gaatcataga gggaaattat caatcgaggt tctccaacat gttcgccagt aaaagcagaa
     4921 actaatgttc caaactgttc cacaataagt aatctctctt cgataacttc ttcataggat
     4981 ttatcatctg ctacaatcag aaaatccaaa tcagaatatt catctaacga ttttgtgata
     5041 taagaaccgc taattgcaag tcctattatc ctactatctc tttttgaaaa ttctaatacg
     5101 ttttgtatca ttttctgttg tgttttagtc ataatataac acctctcact gtcttttatt
     5161 agctatcaat ccaacataat tatgcatagg agtattatat aatgaagaaa taaaaaaaaa
     5221 taggtcactt gacccgaaat aaaaaagcat tgaataatta ttattcaata cttttttgaa
     5281 attatactta tgttgtagga agattcttta atacctctcg ctgattaatt acactaaatc
     5341 ttttgttttc ataagcaatt agatgttctt gtacacattc atttatagca cgattaatgc
     5401 ttctaattgg agcgtttact tcgctagcaa gctgtttttt ggtaagcaga tttaattcac
     5461 ttctataata gtgtaaggat atacacctca gaagtcgttc tttaacactt aaagatgata
     5521 attcttcaat taattcagca tttacaacta atttactaga tatttctcga atcagaaatt
     5581 ttgtagcttc aaaatctccc cttaaccagt ttaaaaaatc atctctatga agtttatata
     5641 cattacaatc tgtgaaagcg acaatttcaa cgggtttttt cccatcatta aattgttcca
     5701 tttctccaaa aaaactacca cttctataaa gatgaatcgt tgagatgttt cctttcacat
     5761 ttaagatgaa tgcttctgca ataccgtcaa tcataagaat tacatattca ttttcttgct
     5821 cagcaaaaag aatagtctct ttctttttat acgttttttt aacaagcttc tttttaatat
     5881 cttcattcat aaaatcaatt aaatgcatat tcatccctcc ttaccaaatc tttgattaaa
     5941 ttatatcatc tttcaattag ttaggtaaga tagtaaaaat atatttacat agaaactatt
     6001 ccattattat tttccagttg tcatttttct gcaaaaactt atcactgtca cgactggaga
     6061 taccagtaag aaatgttagg atatcaatcg ctaaactttc agaatccttt ttacggctca
     6121 aaatgacgta atggtcaagc aatcctttgt tttcatcatc actggttaaa ttgacaatgt
     6181 ttatctcatg ggctatttct ggtagcgttt ctttccattt gccgcgtttt gctttagggt
     6241 caactatgac cgcctatccg ccgaatagta ccgcatagta aacaagcata ttgttactga
     6301 ataatttacc accacccaat gaaccgataa acgcagacgc taacgcattg gtaatagaac
     6361 cctttacccc ttgactagca agggcaggtt ttagatagac attctttcca gtgtccagat
     6421 tgtagctaac atagatatcc tcatgttcgc caagggtttg tgtcgctcca aaaccaagtc
     6481 cagccaaaaa atccgaagtg acatattgaa tataatcatt gatgtaacgt ttgctgacag
     6541 gaataaattc gccatgcaat cccaacatat ccccaaaggg atgcacgagc ttgattgata
     6601 agtcgtcgta aaagtctttg acttcatcac aatgcctttt tagctcgtca agatcctttg
     6661 tagatacacg gacaacatag ctaagtttat acatagattc tttacagtta aaagtctgtt
     6721 tttaaaaaaa tgtataaatt ataaatcaaa atatagatac tatcaatgac ccactaggtg
     6781 caaaaagcat tgatatggtt aaattatcag acgctaaaag ctgggctatc cgaatgagtg
     6841 aagatgggtg tgcttataaa tccattggca attataagcg ctcactgaaa gcgtcgtttt
     6901 tcatggcaat tcaagacgac tgtattagaa agaatccatt taattttcag ttaagtgatg
     6961 ttttggaaga tacaacagaa ccaaaaatcg ctctaacacc agaacaggaa atgggcttat
     7021 tatcctttat ggagagcgat aagacttatt gtaaatatta tgatgatgtt gttatcctct
     7081 tagaaacagg actacgcatt tctgaattat gcggtttgac tttagaattg gatatgaaaa
     7141 ataaagccat tatggtagac catcaattgt tgagtgatac taagattggc tattatgtag
     7201 aagttccaaa gaccacacag ggtaagcgag aaattccaat gactgaaaga gcttatcaat
     7261 catttgaacg tatcgttgaa cggcgtaaga acgctaaagc agaaccaatc attgttgatg
     7321 gttaccagaa cttcctattt cttaaacaag atggattacc aaaacaagca actcattatc
     7381 aaagcatgtt aagagcgtta gtcaagaagt ataacaagac ccatgaggac aagctaccaa
     7441 agattacgcc atatatcttt atgcatacac tctgtacaaa tatggctaac aaaggaatga
     7501 gtcctaatat attgcaatat attatggggc ataaaaacat tactatgacg cttggctatt
     7561 atgcacatgg ttcatttagt tctgcgagag ctgaaatgga acggttataa gcctagtagt
     7621 atgactagag tttactactt atttactact tttgatggca ttcttatacg gttaaatgcc
     7681 aacttatgca aagtatcttc caaaaagaaa atgtcgatac tccttgtaga tacaggatat
     7741 atcgacattt accaacttat gaaaagataa tcaaatttaa tataagtttt tcaaataaaa
     7801 agatactaaa ttctttttcg agaatctata taacattact taagcttggt tggggtataa
     7861 acaattgagt agctactttt gtatggtgtt caactttagt caaaactctt aaataatgca
     7921 aatgatttag attcattaat attcaatctc ttcataagta gtgaaattcg agttatttct
     7981 gcataattat actatgattt atagattaat tc
//
LOCUS       DAKBNN010000174         7909 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000174 DAKBNN010000000
VERSION     DAKBNN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.175"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            111..782
                     /locus_tag="OUB02_003082"
     CDS             111..782
                     /locus_tag="OUB02_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886816.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            779..1909
                     /locus_tag="OUB02_003083"
     CDS             779..1909
                     /locus_tag="OUB02_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8886817.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKIRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFKIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            2324..3169
                     /locus_tag="OUB02_003084"
     CDS             2324..3169
                     /locus_tag="OUB02_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886818.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            3169..4197
                     /locus_tag="OUB02_003085"
     CDS             3169..4197
                     /locus_tag="OUB02_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886819.1"
                     /translation="MYSKFFTLVKKEFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            4200..5213
                     /locus_tag="OUB02_003086"
     CDS             4200..5213
                     /locus_tag="OUB02_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886820.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(5741..6991)
                     /gene="cme"
                     /locus_tag="OUB02_003087"
     CDS             complement(5741..6991)
                     /gene="cme"
                     /locus_tag="OUB02_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HCT8886821.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(7077..7823)
                     /locus_tag="OUB02_003088"
     CDS             complement(7077..7823)
                     /locus_tag="OUB02_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8886822.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
BASE COUNT         2884 a          917 c         1330 g         2778 t
ORIGIN      
        1 atcaaaatgc tataaaaaat actatcaaaa tactataaaa aaatagttga aaaataaata
       61 aattaatata aactaaaata aagcctacaa aaaagaggtg ataaatagaa atggctaaaa
      121 tactaattgt agaagatgaa aaaaggatgc aaagtattat tgtagaatat atgcaaaagg
      181 gaggatatac ttgtataact gctgatgatg gagtcgaggc acttacaata ttaaagagca
      241 acaatataga tttaatgata ttggatatta tgatgccgta tttagatgga ttttcagtgt
      301 gtagagtttc acgtgaaatg actaatatac ctattattat attgacagct aaaggtgaag
      361 aggaggacaa attaaagggt tatgaatatg gcgcagatga ttatataaca aaaccattta
      421 gccccaaagt acttttagca aaagttaatg ccttgttacg tagatacact actgatattc
      481 ccaaaaattc attgagttta ggaaagattt ttattatggt agcatcaagg caagtgtatg
      541 tagaagataa attgattgat ttaacatata aagaatttga attattgcgt ttatttatgg
      601 aaaaccctaa tcaagttttt tccagagaga aacttttaaa ttgtatatgg ggatatgatt
      661 ttgaaggaaa cacacgaact gtagatacac atataaaaac cttacgcaaa aagcttggta
      721 gtgaaggaca ccatatagtt acattaattc gttcaggata taagtttgag gttaaggaat
      781 gaatagttta agaaagatta tagaaaaact gaaaaatgta gatatttcaa attggattaa
      841 acaaaatttt ggactatatt cagttagaaa aaaagtgttt ttactttcta aattatcagg
      901 agcgttaatt atattatttt atctggttgt tgaagaattt tcgataaact ctaaatttga
      961 attttggatt tggtttataa tactggttat atttattatg agtattgatt tcttattggg
     1021 aaaatttatt tcagaaccaa tcacatctat aaataaatct gcaaaaagta tgtcccaact
     1081 tgatttttct aatccatgta ctgtaaatac taatgatgaa tttggagaac tttctaggag
     1141 cctgaataca atgtctacaa atctacaaca ggcattatca gacctagaaa gtgcaaatat
     1201 tcaacttgaa aaggatgtaa ataaggaaag aatgctattg gaacaaagaa aggagttggt
     1261 agatacaatt tcacatgaaa tgaagactcc acttggcatt atacgtgcgt atacagaggg
     1321 gcttatggat gaagttgatg aagaaaaaaa gaaaaattat atgaatgtta taatagaaga
     1381 aactgataga atgaacaata tgattgtatc attattagac ttatctgcat tggaagcagg
     1441 agtttctaaa cttaacccag agcgttttga ttttatagag tttgttgaaa ctgtggcagg
     1501 tcgtctttta attgatatac cagatgccaa tttttatttt acatatgatt tgccagagta
     1561 tgaagttttt gtagtggcag ataaaataag aatggaacaa gtagtagaga atctaattat
     1621 taatgcaaag aagcatgttg tatataatgg taatttagat ttatcgatta catgtgaaaa
     1681 tggcttgctt ttatttaaaa tttataatga tggtaggatt atagagcaag atgagatttc
     1741 aaagatttgg tctaagtttt atcgtagtgt tcaatctcag cgtacaggag gttcaggtct
     1801 agggcttgca attgtttcac aaatacttac aatgcaaggg ttagaatatg gagtgaataa
     1861 tcgtgataga ggagtagagt tttattttat gattccaatt gatgaataat ttagtattaa
     1921 tttataaatt ataagtatct gatgtaaaat atttttcttt gtagacaata gatatctaaa
     1981 gagaataaaa taaaaaagtc taataaaaaa atttaataaa aagcttaatg agaagtcttg
     2041 ttttcctgat gaaaacaaga ttttttatta agctttttat tatttcactt cgatttcaca
     2101 agagagacac tttgatttca ccaaatagaa ataaaataag ttaaataaat taaaaaaata
     2161 taagaagtaa ttaaatacgc ttacaagggg gggcacttat gaaatggtat acatggttga
     2221 ctattatatt gataatattg gtttctattc cgcttaaaat taaatttata aaatggtggg
     2281 atagacgaca aaaagaaaaa aataatgaag gaggctatga tagatgataa aagtagaaga
     2341 gttgaaattt tcttatggaa aagataaaca gatattgcat ggacttaatt ttgaagtaaa
     2401 agaaggagaa atatttggat ttcttggacc taatggttca ggaaaatcca caacacaaaa
     2461 aatcttaaat ggtgttttaa aaggttacaa tggtcatgta tccatttttg gtaaggaagt
     2521 taaagcatac acagaatctt tatatcaaaa gattggtgtt ttatttgaat ttccttattt
     2581 atataccaat ttaagtgcaa ttgataattt agaatatttt tcgtcttttt atcctaaaaa
     2641 tcaaaggaga gatatatatg aactcttaga tttgttagag tttaagaaag agtttataaa
     2701 taagcctgta tcatcctact caaaaggtat gaagcaacgt attagtatgg caagagcatt
     2761 ggtaagtaat ccaaagttgt tatttttaga tgagcctact agtggacttg acccatctgg
     2821 agctgtacta tttcgtaaga ttattgaaga agaacgaaaa aaaggaacaa ctatcttttt
     2881 gactactcat aatatgctag atgcagattt aatgtgtaac aatgtggctt ttattgcaga
     2941 tggaaagatt atggtaatag ataaacccaa aaatttaaaa atgaaaaata gcaataataa
     3001 agttgaagtg gaatttgtat ataatggaaa tcgagatatt gaaattgtag atatacaaga
     3061 gttagaatct ggaataactt ttaaatatga tgaaatctta agtgtacatt caaaagaacc
     3121 tacactagaa gaagttttta ttaaatacac aggaaggatg ttagtgtaat gtactctaaa
     3181 ttttttacct tagtaaaaaa agagttttgg atgatgattt atggtaaatt ttttgtgctg
     3241 acacttggct ctctcatact ttattcgtgt tatattaatt ttggttatgt aaagtttatg
     3301 gatgaagata gttatcacaa taatgtatat ctatataatc cagagaatat aagcatgagt
     3361 gcatctccat taattaatca agtttcttct ataggtgaat taaaagcaaa acttgatgaa
     3421 gatacaagtg cagtttgtat tgatatgagt tccagtaagc cgaaagtaat tttacatgaa
     3481 agtattaaag gagttgacga ttatagagct gactatgcat tgtcattatt atcatcagaa
     3541 aaagaccata atagcaaaat atcagacaac aatgcaataa ttcaaattaa tgataaggat
     3601 acatctgaga taaaacaaag aaaagacatg acttgtgaga ttttattctt tgaaattgta
     3661 gctattggat ttttagggat tgcctcttta ttatttaaag aaaagcagat gggagtaatg
     3721 cgtattactg gtattttacc tgtaaaaaga agtcttttta tattatcaaa gatatttgct
     3781 tttttgatat gtgacctatg ttttacagtt cttttagtag tatttaatat tggatttttt
     3841 gaaggaataa aaatacttcc acaagtatta gttcaagcta gtattttatc aattattatg
     3901 tctttgattg gaataggatg ctcaatgtta ttgagagatt ttaagcaatt ttctcttata
     3961 tatcttgtgg ttgctgtatt cataactaca cctatatttc tatctgcaaa tacatctttt
     4021 gaaatagatt ggattaaata tcatcccttc tatcatgttt atagtcagct taaaaatgcg
     4081 tttttcatga atttttcaaa agaattagtt tattttgtag tgtgttcttt gacaataata
     4141 gttctatttt tacttgtaaa atatattttt aataaagagt tggttaggga gggataatct
     4201 tgataggatt acgttaccag ttaaagagta taaaaaaaga taaaatgtgt ataatttcct
     4261 tctttttacc agttgttatg gcgattttaa taaattttat gggaacaatt gatatatctt
     4321 cattagggga gatacgtttt ggtatagtga aggatacact tcctgtacaa acagagcagt
     4381 ggttgaagaa gtatggtatg gtaagtgtat atgacacaaa aatgcattta atacgtgaaa
     4441 ttaaggatat taaaacggac acaattggag ttgttagaga tggtaaaggg ataaaaactg
     4501 tgctttatgg aaatgagatt aatgctataa aaaatgttgg aaagacattg ccagagatgt
     4561 ttagactaaa gggtgattta aaagatgttg aggttacaat attgcctaaa gatagtattt
     4621 taaaagagtt tcaaaatgtt tttattgcca tgattttaat tactgctatg tttatgggat
     4681 gcacctttaa tgcaatgaat attatatctg aaaaagaaga tggaatatca tttataaatg
     4741 aagttttgcc acagagtaga agtcagtata ttatacaaaa agtatttttg ggtcttttat
     4801 gtgggtgttt atcttcaatg ataactgtat ttatatgttt ggatatttca atattagata
     4861 tgataatgat gttgttactc gttgtatttt cagcatttat ttcatcactt ataggtttat
     4921 ttattggaaa gttgtcagaa ggtcttatgg taggaattgt ttatattaaa atagtaatga
     4981 ttttatttat aggtactcct ttgttaatat atcttctagg ggttaatacc agtggtatag
     5041 ggaatttatg ctatataatg ccttcagttg ctacatttaa tggtattatg gagattatta
     5101 atggtaatgt cattattatc aaagatattg taattttggc tgtacattgt gtagtatggt
     5161 ttatgatata tattatttta gataaaaaaa gaaagttatc ttttagtaga taactcagta
     5221 taattataat tgggacaatt taataggtga tttaatatac tttgtatagt gtaatttatg
     5281 tagtgcaatt tgtatacttt aatttatgta gtgtaatagt tagttttatt ttataattat
     5341 tcaatataat ttagtaagaa gttcagtaca attagagcta cttagtggaa aacttaatag
     5401 gtacttaaac tatataaata ctttattttc ctccatttgt actataaatt taatttatga
     5461 tataaatgga ggtattatta tatgatttga taaatttcat cattataacc tacaagtaaa
     5521 ggtattatta tataaagtta ataaagatag tatcaaaata ttatttgcta taaagagagg
     5581 gactcaaaat aaagataaac ctatttttca atgttgaaat gataaaatat atttaatttc
     5641 atataaaaaa ttttcttcat aaattaaaaa gtccatttta agcccccctc agcccccctc
     5701 atttttaacc agataaatcg agttaaacta tttaaatata ttactcggat tcatttgact
     5761 tagatattcc ttgcttgtaa aattgttttg aataggggat agccaaagca aaaaataaaa
     5821 gtataatccc tgctatgatt attaaattat taatagaaat tacatctgcc attggtccga
     5881 aaatcatcat accaagagga ataaaaccag tatatataat aataggtaag ctaagaactc
     5941 gacctagcat ttgtggtgct acatgctctt gcaacatagt catagctgat gcttgaataa
     6001 ttggaattga aaaattcgta aaaaacataa atataaaaaa ttgccaagct tgtgttacaa
     6061 gacctagaat aattgaaaaa atagcatagg ataatattgc tagaaaaaag gtcttatttc
     6121 tatttttaaa accaccaaaa actcctataa tcaatccacc taaaaccata cctaaaaatc
     6181 caatcatttc aataatagac aggtatatat aacccttgcc aaatgttcgt tcaataagaa
     6241 gtcctataag aaatcctgat ggaacacaaa gaagcatgaa tgctccataa gtaaaaagca
     6301 cttttctaat gaacttatct gaaaaacagt actttatccc tgataccata tctgcaaaaa
     6361 atgagacttt ttcagtttgt tttggtatat catgtttagg aattttgatg aatatcaata
     6421 atacaatagc aataattgca gtaaatacat caattaaaag aacttgattt attttattac
     6481 caaatccaag tattgcagca gctacaatag gaactataaa ttgtactata gattgtaggc
     6541 tactatttat accattaaaa cgcattaaat gttcttctgg tacaagttga ggaatcattg
     6601 cgtttacagc aggtgtttgt acaccacttc ctagtgaacg tataacagat attactgata
     6661 tggctattaa ttcacttgtt gtgccatttg atgacatcat aaatagaatt agaactaatg
     6721 tagatatagc aattgttgaa tctgatacaa ttatgagtac ctttcgattg tagtagtctg
     6781 cccatacacc agcaaaaaaa gaaattatca tttgaggtat aaaggagcat acagttaaaa
     6841 ctgttaccca tacacctgat gatgtctgaa gtgtgacata ccatatcatt gccatttgta
     6901 caagtgatga gccaaataag gaaatatatt gacttgctaa aaaaagaatg acttttttct
     6961 tccatgtaga attagtattt ttgttttcca tcctattcct cctttgattt gtgtgttgaa
     7021 tttttcttgc tcttaaggat aacacacaaa tcaaagtctg gagttagagt ggagggctag
     7081 gaaaattgtt ttttcataat ttgaagatga gatttcatgg tttttgcatt atgttcagct
     7141 tctttcaatg aagttatcag tttgtcacac actgagataa tttcgtcatc atcttttgga
     7201 gtgtcaagga cagttttaat attagtatta ggattagaag ataaagcatt taacattcgt
     7261 aaaatagctg aaagagaata atttgcacaa cgaagtgcgc ttataatttt aagtcgtttt
     7321 atatcttcat cagtatatac acgataacca ttttgttttc tttttacttt taacaaacca
     7381 ttcatttccc aatttcttaa agtatctatt gtaacattta agtgttctgc agtttctttt
     7441 ctagttaaaa agagtttatt tgtttctgga gtatttccat ttaaaatctg tgcaacaata
     7501 gtaagtgctt cctcggcatt agatttttct atttctaatt gttgcaagta ttgatttaca
     7561 agactaatag ccttatcaaa gtccctattc gctgatgttc ttacaatctc aagggcatct
     7621 tttcttaatc ccctttgtac cacttcaatt ttcaaggctg tacgagcaaa tctaaattgt
     7681 tctatatgaa aatcattaaa gatacgataa ccattggtat tacgcttagg tttaggtatt
     7741 aagtttagtt cttcatataa acgtactgta ttaggatgta caccaattaa atctgctacc
     7801 tctgatgtcg tgtaagtgtc cattagtatt caactccttt tagatatata gtattagtca
     7861 taataacacc aaacaaaagt ctggtgttat aatggagggt ttggctatc
//
LOCUS       DAKBNN010000175         7833 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000175 DAKBNN010000000
VERSION     DAKBNN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.176"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(531..1598)
                     /gene="rsgA"
                     /locus_tag="OUB02_003089"
     CDS             complement(531..1598)
                     /gene="rsgA"
                     /locus_tag="OUB02_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCT8886823.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1595..1996)
                     /locus_tag="OUB02_003090"
     CDS             complement(1595..1996)
                     /locus_tag="OUB02_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HCT8886824.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2792..2888
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2942..3118
                     /locus_tag="OUB02_003091"
     CDS             2942..3118
                     /locus_tag="OUB02_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886825.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3528..4166
                     /locus_tag="OUB02_003092"
     CDS             3528..4166
                     /locus_tag="OUB02_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886826.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIDVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4660..6426)
                     /locus_tag="OUB02_003093"
     CDS             complement(4660..6426)
                     /locus_tag="OUB02_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCT8886827.1"
                     /translation="MKEVEIIKKNDYLENRRRGKYIDRKRVAAYCRVSTDSEDQLNSY
                     KSQVSYYTDLINNNIEWCMVDIYADEAITGTQVNKREDFQRMINDCMNNEIDMIITKS
                     ISRFARNTLDTLKYVRMLKEKNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMRRGELVGFQGCLGYDYHKEDKTLTINEKEAEIVRYIFNRYIEGAGGS
                     IIGQELENLGYKTKYGSTTWSNSTVLGIIKNEKYIGDVLLGKTFTIDPISKRRLDNLG
                     EEEKYYVKNNHEPIISREIFDKAHEILNRRSKNRGKYEAGVTKREKYSRKYAFSCMME
                     CGFCGGTLTRRNWHSGSEYSKVIWQCVVATKKGKKYCPESKGISERVIENAFVESYRL
                     LCDEKKSVLEELLNRINDTVGSSEVNNKIKKIEKELSVIEKKINNLVDMRLDDIIEKE
                     TYEIKYQELIDNQEKLLNERNKLNVVAKNEKSMKQRLKEFKKTLEENEVLDKFDRGVF
                     ESVVEKVIIGEKNKDGENDPTKITFIYKTGFKNTLSSDEFKIPRKNARGRHKRKELCL
                     HGNNEVENLCSHSSSNTCGNCS"
     gene            complement(6495..6641)
                     /locus_tag="OUB02_003094"
     CDS             complement(6495..6641)
                     /locus_tag="OUB02_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCT8886828.1"
                     /translation="MKNKLIRYTMQLAMLKQLLEKELISIEEYNIVKNKLMKDYNIIS
                     DITT"
     gene            complement(7054..7293)
                     /locus_tag="OUB02_003095"
     CDS             complement(7054..7293)
                     /locus_tag="OUB02_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8886829.1"
                     /translation="MSSLHKTNKLLSYDVIYAATKGDVISIEIILRNYEAYIIKLCTR
                     TLYDKGGNVYRCLDEEKKSLLELKLIYTILKFKVA"
     gene            complement(7277..7714)
                     /locus_tag="OUB02_003096"
     CDS             complement(7277..7714)
                     /locus_tag="OUB02_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCT8886830.1"
                     /translation="MKNRVISKESIIYEFDSFCKKALKNESIDIERKNKYLRQIQILF
                     CELSEKQINNIFSNDFYFSDCYSFNVKGFKIHITNDILGEAISLLPELRQKIILLSYF
                     IGYNDKEISGFLNVSQDTVWYQRTVGIKTLRKILEVKKNEFAT"
BASE COUNT         2752 a         1242 c          832 g         3007 t
ORIGIN      
        1 tcacctccct aaaataaatt tatgtttata gaaaccaact taacttaatt atattttatc
       61 atcaaaagta tacatttaca aatattagtt aatagtttta taggttcgag cattattttc
      121 aacaaaaaat ttttattata aattaaaaca ataattttta taaatatatt atttgtattt
      181 tataattcat atatttttac tttataaatt agcaatattg cagttttgct cttaagaaac
      241 atctctatca tacataagca tatactctat tatgtattag tataaatatg ccttttctta
      301 tcttacataa gacctcttta taaagaagcc tatcgtaaca aatatgtata cattatcaca
      361 tgtatatatc atcatataaa aaatcaaact ttattaaaaa aattaataaa ttgctttaaa
      421 taataccaca aaccaaattt gccatcaaaa tcacattaaa gtaacaaatt gatggcaaat
      481 ttaatatatt aatagtatat agactcatag aatgaacaaa aaaactacac ttatcttcta
      541 atcttcttat ttatttttga gatattttta aatttctttt ctttttttgc taagtattcg
      601 cttttattgt ctgcaaatac tgcttcagtt ttaagcctac gataggattc ccatctttta
      661 ggcgaaagtt cttgatgagc aatagcttcc cagatagcac agcctggttc tgaggtgtga
      721 gtacaattac gaaaatgaca ttttccgaaa taagcttcta tatctgcaaa acttttatct
      781 aaaccttctg aaacatccca cattcccaac tctctcatac ctggtgtgtc aataacaata
      841 ccaccacctg aaattaaaaa tagctctcgt ctagtcgtgg catgtcttcc tttatcatca
      901 tctatgcgaa tttcctttgt atttaaaaat tctccaccca tcagacaatt aattaatgaa
      961 gattttccaa ctcctgaaga accaagtaag gcaacagtac agcctggttt aatatactta
     1021 gaaatctgtt tataaccatc tggctctata gatgaggtaa caactacatc tacacccaag
     1081 gctatagaat taactacttg taatttacga tgcacattgt cacataagtc tgatttagta
     1141 agtactacta ctgggatagc tccactttcc catgttaaag acagatacct ctccaatcta
     1201 tgaatgttaa aatccttatt cagtgacatg cacaaaaaaa caatgtcaat attagctgca
     1261 accacctgct cctcatttac ctttccagca gccttacgca caaacaagct ttttctatga
     1321 agcacatgat gaataatagc attaccattt ttattattgc aacgatccaa cataacgaaa
     1381 tcaccaactg tgggaaaatc agattgtata gtagcctcaa aacgcagctt tcctgaaacc
     1441 tctgcaaaca gtacaccatt tacacataca gcttgataaa agttcttttc ctgcgagata
     1501 atccgagcaa ggtataaatc atgatattcc atactttgag taataaaatt tctatttaga
     1561 ccatagtctt gtaaattgat ttgtttcata ccattcattc tgtttcctcc ttttccaact
     1621 tttcaatgat tctttctagt gggaaagggg gctgtgccag tggttttaat gcaattgaca
     1681 ttaatttcac tgaattatca tcagctgcaa tacgattaga actaagatgt ccacagcgtg
     1741 tacaacgatg aataattgcc cactcaccat tttttctcac ccacactcca ataggttcca
     1801 tattagcatc acaatctgca gcacggtcac caggaatgat atctacatga agactatgta
     1861 aacaatgtgg acaatgattt ctatgttgtg taccagctcc ttcaggaact acaagacaac
     1921 cacaattttt gcaaataaag ttatccatac aggaattttc ctgataataa gctcgttttc
     1981 tatttttata attcattttt atttcctccg tatcttgtat tttctacaaa aaaagtccac
     2041 aggtatttat atctgccttt ttatgtggta tacatattta cctacacaaa aacagaaaac
     2101 ccctgtggac tagaaaattt agtatcttct atttcaacat aattatattc aaaataatac
     2161 tgtgtaaaat taataaaagt gtagcagatt aaagcccact acaccacaat ttaacaaagt
     2221 aatatgtaat attattattt gttgtctaat gttagttatc tataaaggca gagtaagaca
     2281 caacaatcaa atgtatcaaa aatacactta aaaattctga ttaaacagga cccatatgcg
     2341 tctccaacta tttatttgtt ggttaccatt atatataact ctgacatcag gcaagacact
     2401 ccttcactaa taattattat tgaaatttta tcataaaata taagtatgtt caagtataat
     2461 ttaatatatt catatattta tgtttgtctc attttcttat tctataaaat ctagtggtat
     2521 tttaaaattt aatgttcacc tagtattaat aataaaaatt aactgctatt atttgttttc
     2581 tcattttatg cctgttgctc aatttatctc acttaaatta ataacaactg tcttttcata
     2641 caaaacctac aatcaagttt ttccatatat tctacatgca tagtctattt ttttgatata
     2701 aaataacgtt catatttgta aatttattta attttcaaat aagtatattg aataataagc
     2761 aattctattg tattattgta attgtagaat aaaactaaat cggcaaaact agagaaattt
     2821 agtgacgcaa agctataggg actaacgctt ataaaataaa ctatgagtta tgtcagccag
     2881 ttgccaaaaa gagatattct ttttgttttt atgaaagttt tttaggggga taataatata
     2941 tatgtttaga gataaaatgg atagatgtac acatatgttg actgcttata ttggtagttc
     3001 atatgattat tgtgatttta tagatacaca actagatgat tttatactag agtacggaga
     3061 aaatgtggta gaatcttgtt tgcatcaagt gatggtatta gtaagtaagt ataattaaat
     3121 agatttggtt ttctagagaa ccctttttag ttttatctac tttaaatagc cttatgttag
     3181 aaagctaatt aagatagatt tctagtattc ataattttat tttttattta aaatacatat
     3241 aataaagatg taaatttgta tcaaataata aatatgagaa cttaaattct cttatctatg
     3301 tgaaaataaa catctcttaa aatgaaattc tgcaaacata aatgattttt tagtttgaag
     3361 gattagatta acaaaattat aaatttgcaa ataagctata ttaaaaaata taataatttt
     3421 ttatataact tattctttta taacttttaa ttttaaaaga ggagaatgta tataagagta
     3481 ttcaatgtac tagaaaaaat caaaagaaat attagaagaa atggatattg agtaatcaaa
     3541 gaaaagattt tgatattaca aatgatatgt ttcaagaaag cattcctatc acaacaaaaa
     3601 ttattcttga agacatgcct tcaaatgatg aattaaatca tgttttttca aaaggttgtg
     3661 aacgaaaaat gaaaaagaga aaaattgtat taatcacact actattaata gatgttttgt
     3721 tattaggaag tatcctatat aatctttttt taggaaaaac agctaatatt tcaatgctaa
     3781 aggaatcttg gaattttgat ataccaatac ctaacaaaga aattgaagta tttgatactc
     3841 aagatagtat taatggagat ggacaaagtt attttattca aggattttct gaaaaaaatt
     3901 tcaaaaaagt ttttaattta aaaggtggta tagttgtaag caaagataat attaatgaaa
     3961 tagaaaaata tattgataaa tttaaaagag attctgttaa cattaataaa agtaataaaa
     4021 ataaaattga agaagatttt aaaaaatata agttagaagt taaaaaagat gataagtata
     4081 tatataagag aaattatgaa aactatgtag ttttaattat aaaaaaagat gaacaaaaat
     4141 tatacagttt aatatggaat cagtaggtac atatgtatta ttaagaatta taaataataa
     4201 aaataaattt tagtcaatat acaaaaacct tttaaattgt ttggaaatca cctcctcatt
     4261 tgttataaaa acatacaaca aatgaagagg tgattttatg gtttaaaaaa aaataattat
     4321 aagtggatat gtgctttcac atgtttatca acatttgtaa tgcctatatt tgctaatgaa
     4381 ttataatata aaaatattaa ataatattca actatagatt atgtaaatta ttctatgaaa
     4441 aaaatgagtt gtataaaatc tggctactgt agattgacga tttcttttaa aattacaatt
     4501 ataataagtt ttatagaatt aaaaataaaa ctttagagat ttttgataac attgtagact
     4561 aaatttgcca ccaaaatcgc tttacagtgg caaatcggtg gcaaatacaa tatattatta
     4621 gtatataatc tatataaaca ttactattgt ttgattatct tagctacagt ttccacatgt
     4681 gtttgaacta ctatgtgaac ataaattttc aacctcatta ttaccatgta aacacaattc
     4741 ttttctttta tgtctacccc tagcattttt tctcggtatc ttaaattcat cactacttaa
     4801 agtattttta aatccagttt tatatataaa agtaattttt gttggatcat tttctccatc
     4861 tttattcttc tcacctataa ttactttttc aacaacactt tcaaatacac ctctatcaaa
     4921 tttatctaaa acttcatttt cttctaaagt ctttttgaat tctttaagtc gctgtttcat
     4981 actcttttca tttttagcta ccacatttaa cttatttctt tcatttaaaa gtttttcttg
     5041 attatctatt agttcttggt attttatttc ataagtttct ttctctataa tatcatctaa
     5101 tctcatatca actaaattat ttattttttt ctctattact gataattctt tttctatttt
     5161 ttttatttta ttattaactt cactacttcc tacagtatca ttaattctat ttaacaattc
     5221 ttctaataca gatttctttt cgtcacataa taaacgataa ctttcaacaa aagcattttc
     5281 tattacacgc tctgaaatcc ccttactttc tggacaatat tttttaccct ttttagtagc
     5341 aactacacat tgccaaatta ctttagaata ctcagatcca ctatgccaat ttcgtcttgt
     5401 taaagttcct ccacaaaatc cacattccat catacaacta aaagcatatt tacgactata
     5461 tttctctctc tttgtaactc ccgcttcata tttacctcta tttttacttc ttctatttaa
     5521 tatctcatgt gctttatcaa aaatttctct actaattata ggttcatgat tattcttcac
     5581 ataatacttt tcttcttcac ctaaattatc taatcgtctt ttagatatcg gatctatagt
     5641 aaaagttttt cccaataata catctccaat atatttttca ttttttatta ttcctaaaac
     5701 tgtagaatta ctccatgttg tactaccata cttagtttta taacctagat tctccaattc
     5761 ttgtccaata attgaaccgc ctgcaccttc aatatatcta ttaaatatgt atctgacaat
     5821 ctctgcttct ttttcattta ttgttaatgt tttatcttct ttatgataat catatcccaa
     5881 acatccttga aatcctacta attctcctcg tctcattttc atctttaaac ctttttttac
     5941 attagctgat atgttttcta cttcttgttg tgcaacagaa cttagaacaa ctaaaagtaa
     6001 ttctccatcc attgttagag tatttatttt ttcatcttca aaatataccg ctatattctt
     6061 ctcttttaac atacgaacat attttaatgt atctaatgta tttctagcaa aacgagaaat
     6121 tgacttagta attatcatat caatttcatt attcatacaa tcatttatca ttctttgaaa
     6181 atcttctctt ttatttacct gtgttcctgt aattgcttca tctgcatata tatcaaccat
     6241 acaccattct atattattat ttattaaatc agtataatac gatacctgtg atttataact
     6301 attaagttga tcttcactat ctgtactaac acgacaatat gctgctaccc ttttacggtc
     6361 aatatattta cctcttcttc gattttctaa ataatcattc ttctttatta tttccacttc
     6421 tttcatatat tatatagcct cccaactttg tattcccttt gttgtatata taatacttca
     6481 aagcaatata caattcaagt tgttatatca gaaataatat tataatcttt cattaattta
     6541 ttttttacta tattatactc ctcaatggaa ataagttctt tttcaagaag ttgttttaac
     6601 atggctaatt gcatagtata tctaattaat ttatttttca tttttacaca tccttaatta
     6661 aatattctat atttcagtaa acaattatac ggctctggca agctccacag gaatttcacc
     6721 ttatcccatt cttatgggta taccgttatt ttaaacggga gtatcattat actaatatat
     6781 aaatcatggc agtcattttg ccaaaatgac ttacaggtca ctaactttaa tcgctctctt
     6841 ctattatata aaaggtcttg gcgagttagt ctgttggctc attatatatt aaatgtatat
     6901 aattgtttta ttcaattttc aaagatcaat ctagacctat ataatatcgg tctattaaaa
     6961 tatattaaaa tttaatatac tttaatatgc cgatatttcc taagctagag agagtataga
     7021 aggggtctaa atactcctct agccaaattt atattatgct actttaaatt ttaagattgt
     7081 ataaatcagt tttaactcaa gtaaactctt tttttcttca tctaaacatc tataaacatt
     7141 cccaccttta tcatatagag ttcttgtaca aagtttaata atataagctt cataatttct
     7201 tagaattatc tctatagaaa ttacatctcc ttttgtagct gcataaataa catcatatga
     7261 taataattta ttagttttat gtagcgaact catttttctt tacctccaat atttttctca
     7321 atgttttgat tccaacagtt ctttgatacc atactgtatc ttggctaaca tttaaaaatc
     7381 ctgaaatttc tttatcgtta taaccaatga aatatgaaag taaaattatt ttctgtctta
     7441 attctggtaa taagctgata gcctctccta aaatatcgtt tgttatatga attttaaatc
     7501 cttttacatt aaagctataa caatcactaa aataaaaatc atttgaaaaa atattattta
     7561 tttgcttttc tgataattca caaaataaga tttgtatttg cctaagatat ttattttttc
     7621 tctcaatatc aatagattca ttttttaatg cttttttaca aaagctatca aactcatata
     7681 tgatagattc cttgcttatt actctgtttt tcatattctc acctcctctc gcattaagct
     7741 aaagtggtga taaaccgctt tcttttcccc ttctactatt accacgaata gcataataat
     7801 aattccgtaa aatatataaa aaaagcacat aat
//
LOCUS       DAKBNN010000176         7826 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000176 DAKBNN010000000
VERSION     DAKBNN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.177"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(258..920)
                     /locus_tag="OUB02_003097"
                     /pseudo
     CDS             complement(258..920)
                     /locus_tag="OUB02_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1080..1616)
                     /locus_tag="OUB02_003098"
     CDS             complement(1080..1616)
                     /locus_tag="OUB02_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCT8886831.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1689..2102)
                     /locus_tag="OUB02_003099"
     CDS             complement(1689..2102)
                     /locus_tag="OUB02_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HCT8886832.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIIFIHDRECSECQEKVK"
     gene            2595..3233
                     /locus_tag="OUB02_003100"
     CDS             2595..3233
                     /locus_tag="OUB02_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCT8886833.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQDSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3841..4020)
                     /locus_tag="OUB02_003101"
     CDS             complement(3841..4020)
                     /locus_tag="OUB02_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8886834.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4246..4779)
                     /locus_tag="OUB02_003102"
     CDS             complement(4246..4779)
                     /locus_tag="OUB02_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCT8886835.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQITVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5096..5446
                     /locus_tag="OUB02_003103"
     CDS             5096..5446
                     /locus_tag="OUB02_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886836.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVNRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            5415..5734
                     /locus_tag="OUB02_003104"
                     /pseudo
     CDS             5415..5734
                     /locus_tag="OUB02_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(6364..6936)
                     /locus_tag="OUB02_003105"
     CDS             complement(6364..6936)
                     /locus_tag="OUB02_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCT8886837.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6998..7261)
                     /locus_tag="OUB02_003106"
     CDS             complement(6998..7261)
                     /locus_tag="OUB02_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HCT8886838.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNIYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7261..7482)
                     /locus_tag="OUB02_003107"
     CDS             complement(7261..7482)
                     /locus_tag="OUB02_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HCT8886839.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2741 a         1317 c          923 g         2845 t
ORIGIN      
        1 ttacctgctg attgcccatt ttaaagtact taggatttaa ccttatacca tctaaccaat
       61 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      121 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      181 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      241 tgcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      301 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      361 ttatggactt cttcaagtga aaacataaca cccgccagtg gtgcatgaaa ggctgctgat
      421 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      481 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      541 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      601 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      661 gaaatcattg gctcccagtc caccaacttt gatacaatcc atgcaagtac tgcaagtact
      721 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      781 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      841 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      901 tgagatgtat cctttttcat actattcctc cttcatccaa taattctaca caatctgaat
      961 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1021 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1081 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1141 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1201 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1261 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1321 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1381 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1441 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1501 tcaatagcag tcttatccca tatacttttc aataatataa tgacatcttt tgcactttta
     1561 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1621 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaacag
     1681 tgatttagct atttcacttt ttcttgacac tcgctacatt ctctatcatg aataaatata
     1741 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1801 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1861 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1921 aacccttttt gacagacaca atcctttatt ttacacttac ggcagtgttc tggctttcct
     1981 gtatcgccat tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2041 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2101 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2161 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2221 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2281 atacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2341 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2401 cttagacaat acataagatt cattaataaa aaagttctca atatagtcat taataaaaac
     2461 gttctcaata tagatatatt taaccatgtt ttgaatagca gataattata tgattaaaaa
     2521 ttaaatatga catagcgttt gtggaatata acacctaaat tatgttataa taataaaaca
     2581 ggagtgtgat agtgatggat gaaaaaatat tagagttatt acaggaaatg caaggtaata
     2641 ttagtaatat gcaaggtagt atcagtggta tcgaaggcac tattagcgat atgcaaagca
     2701 atatcaatgg tatcgaaggc actattagcg atatgcaaag caatatcaat ggtatcgaag
     2761 gcactattag tagtatgcaa ggcaatatca atggtatcga aaacactatt agtggtatgc
     2821 aaggcactat tggcgatatg caaggtagta tcagtggtat gaatggcact attagcgata
     2881 tgcaaggcag tatcagtagt atggatggca ctattaatag tatgcaagac agtattactg
     2941 atatgcaagt tgaaattaaa gaagtcaata gtaggttaga caatatggaa cctgatatta
     3001 aagacttaaa aactggacaa gttgaaacta gtagtagact tgatactatt cagagtgatg
     3061 ttacagaaat taagtcagat gtaaaaatta acagaaatac attagataca gtgattaaac
     3121 atacctcttc attagtaaaa gatacatcag atattaaaaa tgatttaaat aatatagaga
     3181 tagtcacatc taagaattcg aatgatattg ctaaattaaa atcagttaaa taatatacta
     3241 tagtaaaaaa tacctgaaaa tttcacatac ttctttaaag ttataccaaa ttttatacag
     3301 aaatactgat tatccgacaa cacactttac ttataaataa tttattaaaa aaatactcat
     3361 tttatcctaa aattaacacg actaaatgag tcttgatata ataataaaaa ataagggaga
     3421 ttggacacta aaaagttgtg tatctccctt atatcctata atatagttat ataatttttc
     3481 agcctaaaat aaaagtccat ctttgattaa aacacatccc cttatcactt ataaaaggat
     3541 aaaatcttgt tttcattttt ccatttatat cttcttgctt aagagtaata attttttctt
     3601 ttgatacttt aagtgtttgg ttaaatttaa ccaatcgact aagttttgtt caacatctat
     3661 ttttcatatt ttccaatact taatctattt tttataaata attctttcat ttctaactca
     3721 cttacatttt ttacataaaa cgaagcatat aaatttcaac cttatcttgt atggttctta
     3781 atacttaatg agtagcttaa gtatcaaaca ttataaattt tccccttaca tttaacactc
     3841 ttaccatttc caaaatatac aaaatatatt ttgaacattt gtacaatgat gaaatacaat
     3901 acatgaaaat acaatatcat aactgttgtc taaaaataga agttcttcaa cataaccttt
     3961 cacaaaaaac atattatcta aatatttttt gtctgctcct tgttttcctg tttgtagcat
     4021 ataaattact gcatctaaac atataactac tcacacgaaa aatgaaaata aacttccaaa
     4081 atacaagctc taaaaactac ctctaaagtt gtataatgtt tactaggaat aaggttgtat
     4141 aacatatagt ttaaataatc attttttaaa attgcaagtt attgctttca aaattagatg
     4201 tttgtatatc aaataaatga ttaagaattt ctaaatttaa tggctttata atgcactccc
     4261 tttctttggg ataaaataat gttgcacaga tactttttct aaagataaca attttatttt
     4321 tctatcaatt ccaccagcat acccagtcaa actcccattt gttcctacaa cacgatgaca
     4381 tggaacaata atagagattc cattatgccc tactgcacca ccgattgctt gtgctgacat
     4441 tttagatatt cctttttgtt gagcaacttt atatgcaatt tctccataag tgattgtttt
     4501 accatatggt atttgttgta aaattttcca cactgtaatc tgaaaaggag tacctaccat
     4561 atggagtgat ggtgtaaaat tgggttcttt tcccgaaaaa taaatatcta accatttctt
     4621 tgtctgctcg aaaattggca attctttttc ttgatgttct tggtcaagat tataagcata
     4681 atacttttga ttctcaaacc aaagaccagt taacccaata ttatctgatg ctaaaagaat
     4741 atttccaatt ggactatggt aatagctaat atattgcata caattcctcc ttatattctg
     4801 aaaacttcac tcaaatcatt agacacttct tttctatcta ccattatgta aaaacatact
     4861 aatataattt ccttctaact acactttcgt tataatatct atcctcatat catattagtt
     4921 ttatatttac ctgcatctgt tcatcctttt ttccctttcc ttttgatgaa tataaaattc
     4981 atacatttaa gcttcctact gtattaactt ttatagaagt gtatattata attttatcac
     5041 taaatagacc aaaaaaatat cgatataaca tcaagtaata tcataatact ttcaaatggg
     5101 aaaagtaaga ataggctgta aaaaagaaaa gtatttttat gttagaaatc cagtaaaatc
     5161 gttagtaaat agaataacag aaaacgaaca attcgctatt tatcatttta cacataaata
     5221 taattggcgc aataaagtgt tttctattct acaacgatta ataaggttag aaaaaagtaa
     5281 aaatgaactg tatatgtatg aagcattagt ttctgtttct ttcctttggc ttattatgat
     5341 aaaaaatatt gttttgccac caataaaaca caaaaatata attaatatcc atatgcaaaa
     5401 aattttacgt tatattgaaa aatactattg ttttacatta tattgaaggg tactattgtg
     5461 aaaacttaat tttggagtac tatcaagcag tgataatatc aataaatcag agtgttccag
     5521 aggatttgaa ctaagtttaa atactatacc ttgtaaatat tttactgagt tttgtttact
     5581 aaaagcaact caatttctaa aaggcacaaa tgagctaata agtgacattg ctataaaagt
     5641 tgtttttcat caaataatct attttagtaa atgttttaaa gaaaaaacgg gatattctcc
     5701 aaaagtgtac agaaatataa aaaaagagat atgatttttt atttcttttg attatccaaa
     5761 gagtaaataa aaaatattgt aaaaaactta ctaacaacgg caatgctttg aactatggta
     5821 atactttgaa cttttgattg gacttttttt gctaaaataa tatatttaaa tagcaaagta
     5881 atctgtttaa tcaataaaaa ataagagata aaagaaatca actaaaactt ttacctctta
     5941 ttttgatagt ttattagagc taatatttac actcttttta tttttgatac ttcattaaaa
     6001 atgatgctta ttctcttttc atttttaata cttcattaga acttatgctt attctctttt
     6061 catttttaat acttcattag aacttatgct tattctcttt tcacttttaa ttcttcatta
     6121 gaacttattc ttactctcca ttcactttta attcttcatt agaacttatt cttactctcc
     6181 attcactttt aattcttcat tagaacttat tcttactctc cattcacttt taattcttca
     6241 ttaaaaatga tgcttattct ccattcactt ttgatacttc attaaaaatg atgcttattc
     6301 tctgttcact tttaattctt tattaaaact gatgcttact ctccattcac ttttaattat
     6361 tcattaaaac tgatgcttgc actctgtaaa ctcataaaca tccattaatg cttcaccaaa
     6421 tctttgatag tgaactactt ccctctcacc caaaaatctc aatatatctt tcaaatcagg
     6481 gtcatcagtt aaatttatta attgatagta cgtagccaat gctttttgtt cagcagccat
     6541 atcctcatgt aaatcagtta ccgaatcgcc aaagacatta atataacttg tagtaaagtt
     6601 tactccatta gaatctactg gctgaagacc atgtccaaaa ttagcataat tactaccaag
     6661 tccagcagcc ttaagctctt ctggagtaca attgcctatt aattgatata acactgaaga
     6721 aattatctca acatgagcca tttcctctgt acctatatca gttaaaagtg cacgagattt
     6781 tccagttggc atagtatatc tttgttgaag atatctagtt gcagctgcca actcaccatt
     6841 aggtcctccc aactgagtca tgatatattt tgccattcta gggtctgctt gtcttatgtt
     6901 aactggatgt tccagtgttt tttgataaat ccacatatta tcttactcct ccttaatata
     6961 tatttaataa taaatctaat tttaaagttt aataatttta tttataaaat gatttgtccc
     7021 atggccaagg ttcagatatc catgaccaag gatattcact tgattcatat ccaaaattag
     7081 ttaatgctcc atatttagat tcataatcac acacagcttg tgtaaattta tcgcagtata
     7141 tgttgtatga atcaagagct ttcttatccc ttggattgtt atctaaatat aaatttaatt
     7201 ctatacaggc aaattgatat tctgaaattc tctctaaaag ttctcttcta tttaatgaca
     7261 ttagtacatg ttaccccctt ttttagctct aactctattt ttaggatttg catatagcca
     7321 atcattatag ttatttgact ctattaaatc taattcagga aataaagtac ctctttttaa
     7381 tgctgttttt ggattgtatg tgtcatcata aatttgacga ttgatgtatg gtctagcaaa
     7441 ttcacaacaa tagtcttgtt tatcctcttt taaatatccc attaattatc ctccttaatt
     7501 tttagaattt taaaatagca ttatttttaa aaattgtata ctggttctat attataactg
     7561 gtagttttaa taggtcttta ataagattaa atgattaaat aagtatcttt ttttcttatt
     7621 ttacctctaa aatttttcta attaatcttt cataaatatc tattttaaat aaataatata
     7681 gagtttatac aattttttat agcttaatta ctatttacta tatattattc atttttaaat
     7741 aaaatgtgat tattttttat taaaaacgat ataaatatac aatttaatac tattttttta
     7801 taaataaact tgtattaaaa tacatt
//
LOCUS       DAKBNN010000177         7744 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000177 DAKBNN010000000
VERSION     DAKBNN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.178"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            42..1913
                     /locus_tag="OUB02_003108"
     CDS             42..1913
                     /locus_tag="OUB02_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HCT8886840.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2020..2727
                     /locus_tag="OUB02_003109"
     CDS             2020..2727
                     /locus_tag="OUB02_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCT8886841.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSENEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            2760..4595
                     /locus_tag="OUB02_003110"
     CDS             2760..4595
                     /locus_tag="OUB02_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HCT8886842.1"
                     /translation="MKISKKIVSLLTITFLTIMLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWTKSESAVLVNDNSIADALSATPFAKAKDVPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(4643..5038)
                     /locus_tag="OUB02_003111"
     CDS             complement(4643..5038)
                     /locus_tag="OUB02_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCT8886843.1"
                     /translation="MILKKYKETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            5266..7677
                     /locus_tag="OUB02_003112"
     CDS             5266..7677
                     /locus_tag="OUB02_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HCT8886844.1"
                     /translation="MRKYKLKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATEPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESDGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
BASE COUNT         3234 a          939 c         1370 g         2201 t
ORIGIN      
        1 aaaaaataat aaaaatttga attttttagg gggaaaatac catgaataaa aaaaatcttt
       61 ctgtaattat ggctgctgca atgataagta catcagtagc tccagttttt gctgcagaaa
      121 ctacacaggt aaaaaaagaa acaataacta agaaagaggc tacagaatta gtttcgaaag
      181 ttagaggttt aatgtctcaa aagtatacag gaggttctca agttggacaa ccaatatatg
      241 aaataaaagt tggtgagact ttatcaaaat taaaaataat aactaatata gatgaattag
      301 aaaaattagt aaatgctttg ggagaaaata aagaacttat tgtaactata acagataaag
      361 ggcatataac aaatagtgca aatgaagtag ttgcagaagc aactgaaaaa tatgaaaatt
      421 cagcagacct ttccgctgaa gctaattcta taacagaaaa agctaaaact gaaaccaatg
      481 gaatttataa agttgcagat gtaaaagctt catatgatag tgctaaagat aagttagtta
      541 taactttaag agataaaaca gaaacagtaa cttctaaaac tatatatgta ggtattggtg
      601 atgaaaaagt tgatttaaca gcaaatccag ttgattcaac tggaacaaat ttagaccctt
      661 ctgcagaagg atttagagta aataaaatcg ataaactagg tgtagcagga gctaaaaata
      721 ttgatgatgt ccaattggcc gaaataacta taaaaaatag tgacctaaat acagtttcac
      781 cacaagattt atatgatgga tatagattga ctgttaaagg taatatggta gcaaatggta
      841 catcaaagtc aattagtgat atttcagcaa aagattcaga aacaggaaaa tataaattta
      901 ctattaagta tactgatgca tctggaaaag caacagagct tactgtagaa agtactaatg
      961 aaaaagattt aaaagatgcc aaagctgcat tagaaggtaa ttcaaaggtt aaattgatag
     1021 ctggagatga tagatatgca actgcagtgg ctatagcaaa acaaacaaaa tatactgaca
     1081 atatagttat agttaattca aataaactag ttgatggatt agcagctaca ccacttgctc
     1141 aatctaaaaa agcacctata ttattagcat ccgataatga aataccaaaa gtaactttag
     1201 attatataaa agatataatt aagaaaagcc catcagctaa aatatatata gtaggtggag
     1261 aatcagcagt atcaaataca gctaaaaagc aattagaatc agtaactaag aatgttgaaa
     1321 gactagctgg agatgataga catatgactt ctgtagcagt agcaaaagct atggggtctt
     1381 ttaaagatgc atttgtagta ggtgcgaaag gggaggctga tgctatgagt atagctgcca
     1441 aagctgctga acttaaggct cctataatag taaatggctg gaatgatctt tcagcagacg
     1501 ctatcaaatt gatggatgga aaagagattg gtatagttgg tggttctaac aatgtatcta
     1561 gtcaaattga aaatcaactt gctgatattg ataaagatag aaaagttcaa agagttgaag
     1621 gagaaacaag acatgatacc aatgctaagg ttatagaaac atattatggc aagctagata
     1681 aactatatat agcaaaagat ggatatggaa ataatggtat gctagtagat gcattagcag
     1741 caggacctct agcagcaggt aaaggtccaa tacttctagc taaaactgat ataacagact
     1801 cacaaaagaa tgcacttagt aaaaaattaa atcttggtgc agaagtaact caaataggta
     1861 atggagttga attgacagta atacaaaaga tagctaaaat actaggttgg taataactta
     1921 tactgtggag aagattcctt aattaataca ggaatctttt tctataatta tgctatttat
     1981 tgtaaattat aacattttat aaagtaggag gatgtagtag tggtagatgc aactttattt
     2041 tttacaccac atcaagatga tgaaacctta agtatgggaa gtgctataat tgaacatgtt
     2101 gaaaaaagtg atacacatgt catattatgt acagatggta gtaaatctat aataagaaaa
     2161 gttttggatg atggagggtc atgttcttat catattaaag atgttcataa atactcttta
     2221 tcagaaaatg aattttcaaa agatagagat gaagaattta aggatagctg tgaagcaatg
     2281 ggtgtaaaag aaagtaatat acatatagaa gataatagag cacatgatgg tgagttaagt
     2341 aaagaaaagg caagggaaat aatacttaag tacttagaag aatatccaga tgcaaaagta
     2401 aaaactgtta ctccgtttaa agctagtgga atacatgaag accatagggc attaggagaa
     2461 gctgcactag agttatataa agaaggcaaa ataaaagatt taagatttta tgtagaacca
     2521 tatgactata aggattttaa aaaagttaat ccaaatgtag aggtatggaa ggtattacct
     2581 agtcaagaag agaaattatt aagtgcaatg aatgcatata aaaaatggaa tcctgaaagt
     2641 ggacattatg ctataggata ccattcagta aaatctcatt ttgatgagct agctacaaat
     2701 aaaatacagt atgtacatgc tccataaaat attatgaaag aattaggagg taagaattaa
     2761 tgaaaatatc aaaaaagata gtgtccttgt taactataac atttttaact attatgttat
     2821 atggaaatac atctaatgca tctacaaaag atacattaac aggttctgga agatgggaaa
     2881 cagcaataaa aataagtcaa gctggatgga caaagtctga aagtgctgta ttagtaaatg
     2941 ataattcaat agcagatgct ttatcagcta ctccatttgc aaaggcaaaa gatgtaccaa
     3001 tattattaac tcaaagtaat aaattggata gtagaacaaa agcagaatta aaaagacttg
     3061 gtgtgaaaaa tgtatattta ataggtggtt caattgcatt aagttcagag attgaaaagc
     3121 agttaaatgc agaaaatata agttttgaaa gaatatctgg aaatagtaga tatgatactt
     3181 ctttaaaatt agctgaaaag ctagataggg aaaaatctat atctaaaata gtagtagtaa
     3241 atggagaaaa gggtcttgct gatgcagtga gtgttggagc tatagctgct caagaaaaca
     3301 tgccaataat actttctgat tcagagaatg gaactgaagt agctgataat tttatagata
     3361 gtaaagatat agaaaaatcg tatgtaatag gtggtacata ttctatttct aattctgtag
     3421 aaagaagttt accaaatgca acaagaatag caggtagtag tagaagtgag acaaatgcaa
     3481 aaattataga agaattttac aaagatactg atataaaaaa tatttatgta acaaaggatg
     3541 gtacaaggag taagcatgat ttaatagatt ctttagctgt gggtgtatta gcttctaaaa
     3601 atggttctcc aatactttta gcaggaaata agctagatag ttctcaaaaa gatgtgttaa
     3661 acactaaaat tatagataaa gttattcaaa ttggcggatt gggtaatgaa aatgttgtag
     3721 aagatgtaat agatatgcaa gaagaaacta agtatactgt tgaaactatt gatgaactta
     3781 atgttgctat aaaaaaatca gatgcaaatg acatcataag atttaagcca gaaaaagata
     3841 aaaatataac taattctttt aaaatagaaa caaataaagc tataactatt gaatttgatg
     3901 gtatatataa acaatcaata actatagata tgccaaatgg agatataaaa aactttgggg
     3961 acatagataa tgatctagta ataaataata taaaaaatga gactttagtt aacgaaggaa
     4021 gtatacaagg gatagatgtt tatgatgaga atggatgtaa aatagagaat gaggattctg
     4081 gagaaatatg gcttataacg atacttaaaa attcaaaaga tgtatatatt ttgaatcgtg
     4141 gagaaataac taaaatatct aataattctt ctagtgtaac aataaaaaat tcaggtaata
     4201 ttaatactat aagtggaagt gaagaaccat caataagtgg taaaaaacct ataactaatg
     4261 atacagataa agatgaagat aaaaaggcac gtggtttata tcctaaagta gaatcttgta
     4321 tacctgcttt aaaagattat gtaagaataa ctatatcaac aaaaccagag agttcaaagt
     4381 ataaaattta ttatagagta gttgatgaaa aaccatctgc gaaatatgtt ggagaaaaaa
     4441 ttagtactaa aggttggagt atggttacaa tatcaaatca atcttttatt gaaagagcca
     4501 aagatggatc atatatagaa gcagtagaaa tagatacctc aaataatgaa gtttcacgat
     4561 ggggaaaatc tagcattaca gatgatggaa tttagacatt aaacttattc attttgcaga
     4621 ttactattta gtaatctgct atttattttt taaaaattat catcttgcta aacaagtaat
     4681 ttaaaactat aacaactata ttagtaaata tttttacaat aacatcattt acaaaaagca
     4741 aatcaatcat tacatacata attaacattt caacgacacc agaaagtagc ctaaaactaa
     4801 caaaagacaa aaattcctta aataagaact ttatttcaat tcttttactt tcaaacacaa
     4861 aaaacttatt agtaacataa gcaaataaaa cagcaagtat ccatgccaaa gcatttgcaa
     4921 ccataaaatt aaataaaata actctagtaa aaaataaata cgaaactata tttacaagag
     4981 tagtaaatgc tccaaaaaat aaatataata ttgtctcttt atactttttt aaaatcaaat
     5041 ataatcctcc taagctaatt taatataaat atacttaaag atactaacaa acattagtat
     5101 agcacatcta ttaataaatc aatatatctt aaaatattat aaaaaataaa actataatat
     5161 tctataatta atatgtactc ataatccata ttttatcaaa ataaaatatg accttaattt
     5221 agaaatctga tataataaaa attaaataca tataaggggg taaacatgag aaagtacaaa
     5281 ttaaaaaaat tgtcaaagct actagcattg ttgacagttt gctttttaat agtgtcaaca
     5341 atacctgtct cagcagaaaa ccataaaact ctagatggag tagaaactgc agagtattct
     5401 gaaagctatc ttcaatacct agaagatgtc aaaaatggag acacagcaaa atataatgga
     5461 gtaatacctt ttccacatga aatggaaggt acaacacttc gaaataaggg tagaagtagt
     5521 cttccatcag catataaatc aagtgtagca tacaacccaa tggatcttgg tcttactaca
     5581 ccagcaaaaa atcaaggaag tcttaataca tgctggtctt tttcaggtat gtcaacttta
     5641 gaagcatatc ttaaattaaa aggatatgga acgtatgact tatcagaaga acatttaaga
     5701 tggtgggcaa ctggtggaaa atatggatgg aacttagatg atatgtcagg gagttcaaat
     5761 gtaacagcta taggatatct gacagcatgg gcaggtccta agttagaaaa ggacatacca
     5821 tataatctta aatctgaggc acaaggtgca actgaacctt caaatatgga tactgcacct
     5881 actcaattta atgtaacaga tgttgttcgt cttaataaag ataaggaaac tgtaaagaat
     5941 gctataatgc aatatggttc agtgacatca ggttatgcac attattcaac atattttaat
     6001 aaagatgaaa cagcatataa ctgtacaaat aagagagctc cattaaatca cgctgtagcg
     6061 atagtaggat gggatgataa ttattcaaaa gataattttg catctgatgt aaaaccagaa
     6121 tcagatggag catggttagt aaaaagcagt tggggagagt ttaactctat gaaaggattc
     6181 ttctggattt cttatgaaga taaaactcta ctaacagata cagataacta tgcaatgaaa
     6241 tcagtatcaa aaccagatag tgataaaaaa atgtaccaac ttgaatatgc tggtcttagc
     6301 aagataatgt caaataaagt aacagcagca aatgtatttg attttagcag agactctgaa
     6361 aaacttgatt ctgttatgtt tgaaacagac tctgtaggag caaaatatga agtatattat
     6421 gcaccagtgg taaatggagt tcctcaaaac aattcaatga caaaacttgc aagtggaaca
     6481 gtatcatatt ctggatacat aaatgtacct actaattctt acagcttacc aaaaggtaaa
     6541 ggagcaatag tagtagttat agacaacaca gcaaatccta atagagaaaa atcaacttta
     6601 gcatatgaaa ctaatataga tgcatattat ttatatgagg ctaaagcaaa cttaggtgaa
     6661 agctacatac ttcaaaacaa taagtttgag gatataaata catatagtga attttctcct
     6721 tgtaactttg ttataaaagc tataacaaaa acatcttctg gacaagctac ttcaggagaa
     6781 tctttaactg gagcagatag atatgaaaca gcagttaaag ttagtcaaaa aggatggact
     6841 tcttcacaaa atgcagtatt agtaaatgga gacgcaatag tggatgcttt aacagctaca
     6901 ccatttacag cagcaatcga ctctccaatt cttttgacag gaaaagataa cttagattca
     6961 aaaactaagg cagagttaca aagattagga actaaaaaag tttatctaat aggtggagaa
     7021 aattctttaa gcaagaatgt acaaactcaa cttagtaata tgggtatatc agtagaaaga
     7081 atttcaggta gtgatagata caagactagt atatctctag ctcaaaagtt aaatagtata
     7141 aaatctgttt cacaagttgc agtggcaaat ggtgtaaatg gacttgcaga tgcaataagt
     7201 gttggtgcag cagctgctga taacaatatg ccaataatac ttactaatga aaaaagtgag
     7261 ttgcaaggtg ctgatgagtt tttaaattca tcaaaaataa ctaagtctta tataattggt
     7321 ggaacagcta ctttatcatc aaatttagaa agtaagcttt caaatccaac aagacttgca
     7381 ggaagtaata gaaatgaaac taatgctaag ataatagata aattctatcc tagctcagat
     7441 ttgaaatatg cttttgttgt taaagatggt tcaaagagtc aaggagactt gatagatgga
     7501 ctagcagtag gagcattagg tgctaaaact gattcaccag tagttctagt tggaaataag
     7561 ttagatgaaa gtcaaaaaaa tgtacttaag tctaagaaaa tagaaactcc tattagagtt
     7621 ggtggaaatg gaaatgaaaa tgcttttaat gaactaaata ctcttttagg aaaatagtga
     7681 atggaaagat agtaaggata tacattttat tattaaaaaa tgttcaatag aagtttataa
     7741 atat
//
LOCUS       DAKBNN010000178         7735 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000178 DAKBNN010000000
VERSION     DAKBNN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.179"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            325..1161
                     /locus_tag="OUB02_003113"
     CDS             325..1161
                     /locus_tag="OUB02_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCT8886845.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            1445..2440
                     /locus_tag="OUB02_003114"
     CDS             1445..2440
                     /locus_tag="OUB02_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCT8886846.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(2697..3101)
                     /locus_tag="OUB02_003115"
     CDS             complement(2697..3101)
                     /locus_tag="OUB02_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCT8886847.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            4183..5097
                     /gene="queG"
                     /locus_tag="OUB02_003116"
     CDS             4183..5097
                     /gene="queG"
                     /locus_tag="OUB02_003116"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCT8886848.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRNIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYIFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            5203..7440
                     /locus_tag="OUB02_003117"
     CDS             5203..7440
                     /locus_tag="OUB02_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8886849.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            7637..>7735
                     /locus_tag="OUB02_003118"
     CDS             7637..>7735
                     /locus_tag="OUB02_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCT8886850.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEK"
BASE COUNT         3234 a          667 c         1417 g         2417 t
ORIGIN      
        1 aagatgtata cactatctga gaagtgctta gataatatac aaagtatgta agattataat
       61 atttgaaatt gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata
      121 taaattaaat caatgctaga aatatgttaa aagaggaatg agattagttg ggatagaaca
      181 tcccttagag cttatgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc
      241 atatgttggc taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtag
      301 tagtacaaat aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa
      361 ggatttgcta atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt
      421 agtagaaatc caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt
      481 caagaaatta gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg
      541 aatctgggtg gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat
      601 ataagaagga tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta
      661 ggtggtggtg tagattttgg catagataga atagtaaaag gattgaacaa tgccataaag
      721 ggtgatgaaa atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt
      781 tttaaatttg agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt
      841 gtgtgtcttg atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat
      901 ggggttttgg aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta
      961 aatgatagta tgatgccatt tggagacaaa aaagacagac atgctcctat aggggaagga
     1021 aagataggat taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca
     1081 tttttcttag aaactccttt tgatgatgaa ggtcacaaga gagagcttaa aatgataaaa
     1141 gaaatactat ctagtaaata aacctttatt aaaaatatgt gaaatactcc gccctcaaca
     1201 taattagggt ggagatacaa ttatcaaatg tgataattta aattaatgga taggaataac
     1261 aaaaattgta tacaaattaa ctgggtataa agtatgatta tattatgaat agactgatat
     1321 aaaatataat tatattatga atagtatgta aatatattgc aaagattctc ataatttgtt
     1381 gtataattaa agatgacata tttatattat ataaaaaatt attaaaagtt aggtggggaa
     1441 agatatgaga aatttaggca atactaacat gaaaataaag agagttggct ttgggggcat
     1501 tccaatacaa aggattactc aagatgatac taacttagtt ataaatgagt tagaaaaaca
     1561 aggaataaac tttattgata gtgcaagagg gtatacaata agtgaggaag caatcggaat
     1621 tgccattgaa ggaaaaagag ataaattttt cttagctact aaaagtatgt ccagagatta
     1681 tgattcaatg aagagagatg ttgaaataag tttaaataat tttaaaactg attttataga
     1741 tttatatcaa ttccataatg ttaaagaaga ggagtatgat aatcttttta aagataaaat
     1801 ggcttatagt gcccttttag aggcaaagga gcaaggtaag ataaaacata ttggtataac
     1861 tagtcacaat ttaaatacta tagaaaaagc tatagaagat ggaaaatttg acactataca
     1921 attcccttac aatatagttg aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa
     1981 aggaattggg ataatagtaa tgaaaccttt agctggaggt gctttagaca atgcaacact
     2041 ggctattaaa tatatactat ctaaagatta catagatgta gttataccag gtatggagag
     2101 tgttgaacag gttagacaaa atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga
     2161 taataaggaa attgaagaaa taagaaattc tttaggtaaa aaattctgta gaagatgcga
     2221 atattgtatg ccttgtgctg taggaataaa tataccattg agttttttat gtgaaggtta
     2281 ttatactaga tatggattaa aagagtgggc taaagaaaaa tatgaggtta tggatgttaa
     2341 acctactgag tgtattgact gtgggctttg cgagagtaga tgtccatatg aacttcctat
     2401 aagagaaatg cttaagactg ttgttgaaaa gctaggatag attttattta ttgaaatcgg
     2461 tgtattaaat ttactccgat ttctttttat tagatgtggt cttatataca aatattaaat
     2521 ttacatagtt atagctatta tataaattta atatttgttt aatacttatt aaatatttac
     2581 ttaatgcttt ttaaaaacat ttgtttgagt atagacataa cacaattatt attgttgtat
     2641 tagtatagtt tcaaatactg gagtatcaat ataatgtcga ataacacata aaatctttat
     2701 ttttcaaaaa tattatagga tatatcgtat ttagatactg gataaagttc aatttcaacc
     2761 ctagggttat ctttatctat aaaatcaaat aaaagtactg gcttaagttg tttatcattt
     2821 tttattattc cacttttttc aataccatca caaatacttt ttggataatt atgtaaatcc
     2881 cccattctct tatttgggaa gaataatttc aagactgtta ttagattttc ttcaacagtt
     2941 tccccttgat actgttggtt tataaaacct gcaatcatgt tttcgtatgc aagatactta
     3001 gaatattttc cctttgaagg catccatgct tgaccattta tgttatgaag cttaaaatta
     3061 gatttactaa taggtctacc tggaattgtt attttaatca aatttttcac catccttata
     3121 cagtatttat tataatctgt atatgattta aaagcaaatt gttatttaaa gtgtttttac
     3181 agagcttgtt tgtaaaattt tccactttag atgtagtaat ataagataag tattttgctt
     3241 acaaatttta aaatttgata tatgatggac atattcatat tagactatta taagaccttc
     3301 ataatattaa taagtatagt ttagtaaaga gagtaaaaag aaatccatat aagtcaagta
     3361 aagacttaat aaatggcaat gtaaatggga tattaattat aagaaaaaat caatcttttt
     3421 gcattaacta taggaagaat gtcaaaatat aactaatgat atttgaagtt ttagagttta
     3481 tattaaaagg taagaaggtt aagtgggtaa aaaaggttga atagattaaa aaggctaagt
     3541 atctaaaaga gttaagtagg taaaaaagtt aaataagtaa atgaaaaagg ctaagtatat
     3601 aaaagagtta ataggtaaaa aagttaaata agtaaatgaa aaaggctaag tatataaagg
     3661 agttagtaga taaaaaaatt aaatagataa aaagattaag tatctaaaag cgttggtaga
     3721 taaaaaaatt aaataaataa aaaagattaa gtattcaaaa gagttagtag gtaaaataat
     3781 taaatagata aaaagactaa ttatttaaag gagttaagta gatagaaaag ttaaataagt
     3841 aaatgaaaaa gacgaagtat ttaaaagagt tagtaaataa aaaagatgaa gtatctaaaa
     3901 gagttagtag gtaaaaaact aaataggtaa aaagatgaag tatctaaaag agttagtagg
     3961 taaaaaatta aataggtaaa aagacgaagt acctaaaaga gttagtaggt aaaaaaatta
     4021 aatagataaa aagacgaagt atctaaaaga gttaagcagg taagaaaatt gaatagctaa
     4081 aaagattaag tatttaaaag agttaatagc tagaaaaggt taaaaagact aaaaaaaata
     4141 aataaattta aaaagtcaat tagattaaaa aggaataatt ttatggataa taaggaatta
     4201 aaagcatttt gtaatttaat aggattaaac tgtgtgggag tagcaggggt agataagtat
     4261 gataatttag aaaagatact taaggacaga caacaaaaag gttatttaac tggtatggaa
     4321 gaaccaataa ttgagaacag aattaaccca agaaatataa tgaaggatgc taactctata
     4381 atagtctgtg catttccata tcatataaat agacaagaaa agaataaaaa ttcaaattta
     4441 tcaaaatatt gtcatggaaa ggattatcac attgtagtaa aggacttttt acaacaaatt
     4501 tgtgattata tttctaaaac agtaacagat tttaaataca agttatttgc tgataatgga
     4561 ccattagttg acaggtattt agcttattta tcaggaatag gatattttgg aataaataat
     4621 aatatcataa ctgatgaata tggttcttac atatttatag ggtatatagt aaataattat
     4681 aaatttgaag aagataaacc actagaaaaa agttgtttta aatgtggtaa gtgtgtaaaa
     4741 tattgtccgg gaaatgctat attgggaaat tatgatatga accccaaaaa atgtttatca
     4801 tatataactc aaaaaaaagg agatttgtca gagaaagaaa aagttgtttt aaagaatggc
     4861 aaaaaggtat ttggatgcga tatttgtcaa gaagtatgtc cacataatgt tgaaatacca
     4921 acaactcata ttttggaatt taaagaaaat ataatagatt atttggatta tgatgagata
     4981 caaaatattt caaataaaga atttaaaagg agatatggag atagagcttt tagctggaga
     5041 gggagaaata taataaagag aaatatggaa ataattttag ataagtatga tgactaataa
     5101 taagagtctg tttcatatac tttaatgtgt atttacttta acacttgttg tataaaagtc
     5161 atattatatc attaaagtta aatatgggga ggtgggggaa tattgaaata taataataca
     5221 tctgaaataa atccactaga ttatatattg ggtcaagaaa gagctgtaga agctatggaa
     5281 ttagggttga aaataaataa tccagcgtac aacatatata tagctggtga acctggaact
     5341 ggtaaaagta cttatgcctt aaaggtttta aatgagtatg catccaaaaa aaatacgcat
     5401 aaagattggt gttatatata taattttgaa aaccctagag agcctattat agttgaatta
     5461 gaaaaggggt ttggaagaga acttaagaaa gatatggaga aattaattga aagtctttta
     5521 gaagatttta aaaatgcgtt tgaaagtgaa aattttgaaa tagaaaaaaa taaacttctg
     5581 gatgaatatg aaatagaaaa agatatgctt ttgaagcaaa ttaaaaaata tggagaagaa
     5641 aaaggattta aattaaaaca gagtaaaatg ggaatagttt ttatcccttt gaaagaagag
     5701 gaagatgaaa gtgatgagtc tgatgaagag ttttacaaag ctaaaagaga actagaaaat
     5761 atggcgatac aggtcgtata taaaattaga aacttagaag aaatagctga aaaagcagtg
     5821 ttagagcttg aagaagagat agctaaactt gtagtagaac ctcatatcaa gagattgtgt
     5881 gaaaaatatg aaaattatga taagattcaa acttatctgg aaaatataaa gaaagatata
     5941 atagaatata tgtatttatt ctatttagat gaagaggagt taaaagataa gtatgataaa
     6001 gagcatttta ttaaatataa aattaaccta tttgtagaca atggaagctc aaagaataaa
     6061 gaatcagcac ctgttgtagt agagataaac ccaagtccag caaatctatt tggaaaggca
     6121 gagtatgatt atgcaaatgg aaatataaag actgatttca caaaactatt atcaggagca
     6181 tttcataggg ctaatggtgg ttatttagta ctttatgcag accaattact aaaatataca
     6241 atgtcttggg aaattcttaa aaagactttg cagactaaaa aagtaatatt ggaaacacaa
     6301 acagcaatta aaccagaaaa tatgccattg gatgttaaat tagtactgat tggaagccac
     6361 tatatatatg atattttata tagatatgat gaagattttg aaaaatattt taaagtattt
     6421 gtagactttg atagtgaaat ggataaaaat gaagataatg aagaaggaat agcaagattt
     6481 atagcatacc aatgtgataa gaataattta aggcatttta ctaaatcagc agttgaagag
     6541 gttattaaat ttagcactag attatctggt gatttagaaa aactatcaac taagtttaat
     6601 aaaataatgg aagttgtaat agaaggaagt gcttatgcag agtttagaaa ttcggagtat
     6661 acagagcagt gtgatgttaa aaaagcaata agtgaaaaga gaaaaagaat caatagagtg
     6721 gaaacacata tggatgaatc aattgaaaat ggatttactc taatagaaac agagggaaga
     6781 aaagtaggag tgataaatgg tttgtcagtc ttaagtacag gagaatactc ttttggtaga
     6841 gcttcaagga ttacagcgac cactagccca ggaagtaaag gcatagtaaa tatagaaaga
     6901 gaagtaaata tgagtggctc tatacataac aaaggagtac ttatattagg tggctatctt
     6961 tctgagaatt ttgcacagga tttactattg tcactaaatg catatgtttg ttttgaacaa
     7021 aattatggtg gaatagatgg agatagtgca tcttctgcag aattatatgt actattatca
     7081 tctcttagtg gagtatcaat taaacaaaat attgctgtta caggttctat aaatcaaaag
     7141 ggtgatatac aagtagttgg tggtataagt gaaaagatag aaggatttta ttctatatgt
     7201 aaaactaaag ggttaaatgg tgagcaaggc gtcataattc ctagaaaaaa tcaaagaaat
     7261 ctcgttttat cagatgaagt taatgatgca gtgcgagatg gaaaatttaa gatatatact
     7321 gttgaaagag tagaagaggc tatagagata ttgacagatg ttaaatttga agaaataaag
     7381 aaattggtaa aagaaaaatt aatatctttc agcaagatac aaactgtgtc aaaagaataa
     7441 gtttattata gaatttaatg ggtatttatg tataataaaa agcaagaact ttaataggtt
     7501 tttgcttttt ttgtaaatat atataaatta aattcttatt ttgtatgttt tatatattga
     7561 tgtttatttt tttgttgata tgattaaacc atttataaat aggttttata aattagtatt
     7621 ttaaaggggg ataattatga aaaatgtaaa tctaaaagag ataaatgaaa aagatgtaat
     7681 tttggaagaa gagataaata aaaaaggaat ggataagaaa aatataattg agaag
//
LOCUS       DAKBNN010000179         7710 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000179 DAKBNN010000000
VERSION     DAKBNN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.180"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(5..949)
                     /locus_tag="OUB02_003119"
     CDS             complement(5..949)
                     /locus_tag="OUB02_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HCT8886851.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDNNKQISIFD"
     gene            complement(1293..2729)
                     /locus_tag="OUB02_003120"
     CDS             complement(1293..2729)
                     /locus_tag="OUB02_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HCT8886852.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPAKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            complement(2939..4339)
                     /locus_tag="OUB02_003121"
     CDS             complement(2939..4339)
                     /locus_tag="OUB02_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HCT8886853.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSNINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            complement(4366..5712)
                     /locus_tag="OUB02_003122"
     CDS             complement(4366..5712)
                     /locus_tag="OUB02_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HCT8886854.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            complement(6080..6754)
                     /locus_tag="OUB02_003123"
     CDS             complement(6080..6754)
                     /locus_tag="OUB02_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HCT8886855.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTHGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLGHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            complement(6815..7561)
                     /locus_tag="OUB02_003124"
     CDS             complement(6815..7561)
                     /locus_tag="OUB02_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCT8886856.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
BASE COUNT         2539 a         1374 c          701 g         3096 t
ORIGIN      
        1 tattttaatc aaatatacta atttgtttat tattatctac tttaatagtc ttcatatctc
       61 taatgcttat ttttgcatca tctcttaaag tatctatgag taatttatta tcttccttat
      121 aaagcctaca gttttcttct acttttgcat aattaaagtt agcataacaa tacagacaat
      181 aatgtctgca agtattgtat tgtcctatat cgacactttt aacacatcca caaatatctc
      241 tttgtgtact atcttttttt acctctactt cacatcctat aatttttgat attaatctat
      301 catcaataca cttacttttt ccaatcccaa atttacttaa attatattct tcagaacaca
      361 tttcaagtat tatcccatgc ttatgagcaa tcttagaaat ctcttttgat aatgtttcta
      421 tttctgtaat ttctattggc tttatatgta atgctttagt attaaatttt gttttcttat
      481 acaaatcgat aaaacttatt atacattttt ctgtaaatcc atcaagctga ttacataaac
      541 tcgcaaatgc tttgatatga taatctactg tgtacttttc acttaagaaa atcgggtcgt
      601 atcttaaaat tactctttct ttgcctattt ttctagacaa ttccttaaat gaatctatta
      661 cctttctttt atctaaaata catgtctcta cttcttttcc atatgatgtt atagtatagt
      721 gaaaataata tttatagtct cttaatttat ttaaatcata taacattgga gttacatcct
      781 ttgtccaaaa tacaaaacaa tcaacagtat gtggatttaa ctctatctta ctaacctgtt
      841 ttggattcat tggattaatt acataaacaa aaccttcttc aagtctgtta aaaaaccact
      901 tactatagaa agcaggtata tcagttcttc tactcacact tactatcata tttaacctcc
      961 atagtaatat aattatgttc taatatattt ttaatataat tatagctcta aatacttaat
     1021 ttaatcaaaa catttaagat aattccagta tgatttactg attcatttct agcatttaaa
     1081 atatccttaa taaagttata attctttaaa aataaatata cattattcct aaactttcaa
     1141 aactaatttt ataatgaaac taaaaaaggt gctattttca agcaccctac aattttactt
     1201 tttaacaaat ttaatactat acaaatatat caatattaca ttaaaggtaa acactatata
     1261 ttaaaacttt atatcattat ataatacaaa ttttattgta tttggtttgt tttttctggt
     1321 tcatgatgtt tataataata taccgtttct tttccaatta cttcagaacc aagcttctcc
     1381 catttttctc cataggtagc ctctatttgt tcacattctg aatctgttga tgctacaata
     1441 agaccataat taggtctgaa tatagctgtt tcttcctttc catttttata acttaattta
     1501 ccattattta accttaacag gtttccattc ttttgataat taaaattaat tccattaata
     1561 acttctggac tagatgaatc aataaatata taattgttaa agttattttt taacattaaa
     1621 ttacctcctc catgtaccga tttaacgata taatcatagg catctatatt gcatctcaat
     1681 gttaattttg gtatttgaag ggttttaaat acataaactt tatgtttata ttctaagcca
     1741 tcatcccagt tatccttatt atcactaact aaatcaatat tacattctgt aaaataattt
     1801 ccataaaata aagaatatgc ttcttctact ctatctttaa tacttaaata tgtgtcataa
     1861 tctgtattat aataatactg accacacctt cttataatac cagaaaaata actatctttc
     1921 attgaatcaa atgaaaagcc acaataccca cattggtcaa tcgtaatatt attaatttta
     1981 taacttccat acccctcata ccccttaact tgactactta taagaccaac tttctgtatt
     2041 ttttcaattc ttacattatt tatgctagtt ccagtcctaa tcctcattcc attttgacac
     2101 ttagatattc tactattaga aataattgtg tctattcctg tatcaatacc cagcccacaa
     2161 gatgaaactg taatatcatt tacaattgag taatatggta tcctaattcc tgtacccgaa
     2221 aaaccactta tataaatatt ttcataatga cctagacctt taatcatatt cttactgtta
     2281 tcatacagga ttgctgatac attattcatc tcaacattct tttcattctt attgataatc
     2341 atctcataat gatatttagg attacctttt gccggtggtt ctttatttac ttgtatctca
     2401 caactatcag aatagaaatt tatattttta atacattgtt tcttccctgt caatgtaata
     2461 atactcatat tttctactcc acgagttata aattgtgtag tacctgtaga tgaattagaa
     2521 tcaccttgca atacaatttc tttattaatt ttaacaccac ttgtaaatag gtatttacct
     2581 tttggaaaat ataagacaca aaaatctgtt tctatttcat caataatatc ctgcaaagca
     2641 ggacctacgt tgtacgaacc atcattttta atacctcttt ctactacatt aatccatctt
     2701 ccatctttaa tcccagttct tccttccata atattatata ttcctttccc ttttacttag
     2761 tttgaacctc ctatgactaa agtcacagtt ttactacttc atagaaattt acataatcaa
     2821 attaattttt tatattattt ttatattatt caatccatgc tattaccaaa ccatttacat
     2881 taaaactata gtcctagccc tccaataaaa tacatctatt caaatactac gtatttactt
     2941 atttctctat caatttataa tagtgtacag ttattccaaa ctctattttt tcaccaattt
     3001 tctcccatgt tcccccataa tgagcagaaa gaatactttt atctttaata attgttgaca
     3061 taatgtcacc tacctctggt ttatgcatct taatatcctt atcattacta tttatatata
     3121 tatttccttt ttttatcttg actttatccc cataatcttc atcagatata ccaattccat
     3181 tgatattact tagttttcca tcgtaaaata taacagtttg tcgcatatta ttgtacgtta
     3241 aatttccttt ttctgactta acaatccagt cagtagcatc tatattacat gttaatagta
     3301 cattcttggt tttaagtgct tctataagat acaccttatg cttatcctct ttgaaatctt
     3361 cccaattgtc ttcattggta tttgataaaa ctatattact tgattccaat aaattaccat
     3421 agaataaaga atatgcttct ttaactctat tatccaacat taaataatta tcatagctta
     3481 catcataata gtactgccca caccttgtga tatttcctga tatctgaaca ttggatatag
     3541 aatcaaacca aactccacaa tatccacatt ggtcaattgt gatatttgta ataaaattta
     3601 aacctctacc aatattttta attccaactt tttttatttc ttctaccctc ataccattta
     3661 taaaagttcc agtagaaatt atcattccat caacacagcc ccaaacctta ctgttagcaa
     3721 ttgttgaatt ttcccctgta ctaattccaa gtccggatgt aaaaattgta atatcatttg
     3781 ctgtagaatt attaggcata tcaagtgcaa ttccagaaaa accactaaaa gatagatttt
     3841 cataatgacc tggttctccc tgtttatctt cacatataac agcagatata tttctataat
     3901 ttataattac attatgatga aatctaggtt ttccatctgt tggaggttca tgattagatg
     3961 tcatatctct atcacaacta tctgaataaa agcaaatgtt cttaatacag tgtcttacac
     4021 tttttgctgt tatgattgct atattttcac ttttaggatt tttctttcct ttaaaagtaa
     4081 actgtgttac tccagcagta tttttaggaa cattcgctaa aatataagaa tctccacata
     4141 aggttaatcg tttcttaatc ttgacaccac tttcaaataa gtattttcct tttggaaaat
     4201 atataataga atcttctggt acagcatcca taatctcttg caacttaaaa cttacatctt
     4261 ttgtttcatc attacaaatt ccataatcaa gtacattaat ccagccactt atatctttaa
     4321 ttattataga ctcttccatt aatcactacc tcctagtttg ataatttaat cttttatctt
     4381 tttataatag tacatggatt ccccagctaa tacttttaca tcaatctgtt cccatattcc
     4441 accatgtaat ttatttattt cttctgtaga atctagtact gaatatatta cagtattctt
     4501 ttttaatcta ggaaccatta aatacttacc tttatcatct atatacagag tgttctcgat
     4561 tattttgatt aaatcttttt catctatatc agaaatttga acacctccta cagaacaaat
     4621 ctcaccatta taaaatttat aagtatttac gccattttct aacagcaagt taccttttgc
     4681 acattgtata aagttatctg cttcagcata acatgttagc attacattgt ttgtttcatt
     4741 tgccttgatt acatatatct tatgttcatc aagtgaatca ttttccaagg tatcaatatt
     4801 actattcatc aatacaatat tgcaattttc taaaatatct ccataaaaaa tggaataagc
     4861 ttcttctttc ctatcttcca tattctcata agcattataa tcaacacttt tataatactg
     4921 accgcatcta gtaattctgg cagttatctg gctatagcac attttttcaa acataaatcc
     4981 acaatatcca cattggtcta ccataagatt cataatcagg ttaaaaccaa atcctttatt
     5041 attaatacct atttctttta tttcttctat tctcacatta tttaagctaa caccagtagt
     5101 aatctccatt ccatttttac attcgtaaat cttgctacta gaaagcattg acttctctcc
     5161 aagcctcata ccaagaccac atgaactaac agtaatatca ttaccagttg aatagtatgg
     5221 aatttcaatt cctatccctg aaaatccaga aataaacagg tgttcaaaat gagatagtcc
     5281 ttgtgtttct ccatattctg taatagcaga tacattttta ggcggtattt tctctatatc
     5341 atgactatca taataaaaat taatattttt gatacattga cttgttcctg tcaatgcaat
     5401 aatactcata ttagaaactc ctcgagttat aaaattggtt gttcctatga ttgattgttt
     5461 tttatctaaa tttgatactt gattatctcc tccataataa gaatctcctt gaagtgttat
     5521 ttgttgacta attttaatac ctttctcaaa taaataatta ccttttggaa aatatataat
     5581 ggaaccttct agtgacttat cagagacaat atcttgtatc atattactta catcatattt
     5641 cccagtaata tctattttac tgtttaattc tagtacatta atccaatctg gagttttagt
     5701 ttcaatttcc atgattaatt cctccctttt aaatctaaat tattttcact gaaaaaattt
     5761 tttgactata accgaagtga cttttactct ttttgggaat tttgagatga caattttcat
     5821 tttacaacat cattacagtt atattataaa tcaattataa tctcattata tttttactat
     5881 tagcaagtta ttcttaattt tttaatacaa agtttatata atgattaaaa tagttaagct
     5941 ttgtatcaat ataatttaat tccaataaaa aaacactcat ttctgagtgt tctatacaaa
     6001 tttttatttt gaaaaatctt atataatctt acattttaaa aattttataa tattcaattt
     6061 aataccttct ctagtaaaat tatttttgat ttgcatatct taatgaccat gggcaactca
     6121 tcatacacct caatacgcaa aaacactttg aaacatcaat aatagattct ctttttccag
     6181 gtaaagtcca ttcatttcca tgtatagctt ttgcaggaca gttctctaca caaacattgc
     6241 aatccataca ttttgaatca atcaaaggat atctttcaac agaaattgga gcatctgtaa
     6301 gtactgtaca catgctaaaa gcacatccat attcctctgt tacaaacaaa ttattttttc
     6361 ccataaatcc aatgccacta atatttgcta ttgtcttatg tggtaaagga ctaatccctg
     6421 attgcatttc tggattaata taggcttttt caaaatatcc atgtcctaga ttgtttttct
     6481 ccgattgaga atacgctcta tatcctttat tttgaatata ttttgaaatc cagtcagcta
     6541 attcttctac tttctcctct ttttccaaaa actcatcact atctgtaggt aagttattgt
     6601 atatatctgt aataaatttt tttgataacc ctatacaaaa taaaattgct ttagaaaaac
     6661 cgtgcgtttg atttattgga aattcagata tatcaacaaa gcgaacaatg tctacacctt
     6721 tattgtataa aatagatttt aattcatcat tcatatttga ctcctccccc tagataaaaa
     6781 taacattaca cattagtaaa atttattatt ttaattatgt taaccaatac tttgtacatt
     6841 ctttatttct actcataaaa ccatactcaa ttagatatct tctaattgtt gcaaaatcat
     6901 catatataga ttctaaaatc tgattaactt ccttttctga atatttcttc ccacgttcaa
     6961 attgttccaa aatcttagat aaaattacga cctttttctt ttcttttgaa gaaaatactt
     7021 ttaattttaa aggattcata ctttcaaatg atgtatccaa aatgtgtttt ctctcttctt
     7081 cagttactac atatctttca tctaccattc gagcatgttc atggattggt attatagtac
     7141 tacttttatt agatttatct tcaaatacag attcatatag tgctaaatag aacttagcct
     7201 gttttgcttt ttctctgaaa ataaattttt gatggcgtat agtagatgtg gatactccaa
     7261 cctgttttgc tatctcctta tctggtaaat cactaataaa taattgaaat aattgttttt
     7321 gattttcagt caatgtgttg tactttgaat cactattaag caattgctct aaataatcac
     7381 tatgttctaa ttccatatga agttcaatag cttttgaagc ttcaaagaat ctattaccta
     7441 ttttatatat ttctcctacc tcatatgttt ttgtacattt aatgcaacta taacaattta
     7501 aatcatcatt aaatatatag cctcgtttaa tatcatcaat agaaagattt tcaattttca
     7561 tgttactacc tccatttcac agtttacatt tttcataaat atattatatt aaagtttatt
     7621 aatgtaatca acatttttat tttttgttta tcaaatctaa ttatttatta atttgaaatt
     7681 acaaaatata aaaatatatt taaacaaaac
//
LOCUS       DAKBNN010000180         7694 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000180 DAKBNN010000000
VERSION     DAKBNN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.181"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            321..1514
                     /gene="nifV"
                     /locus_tag="OUB02_003125"
     CDS             321..1514
                     /gene="nifV"
                     /locus_tag="OUB02_003125"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HCT8886857.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1539..3464
                     /locus_tag="OUB02_003126"
     CDS             1539..3464
                     /locus_tag="OUB02_003126"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HCT8886858.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3486..4481
                     /locus_tag="OUB02_003127"
     CDS             3486..4481
                     /locus_tag="OUB02_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HCT8886859.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKVNPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4807..5280
                     /locus_tag="OUB02_003128"
     CDS             4807..5280
                     /locus_tag="OUB02_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8886860.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5314..5808
                     /locus_tag="OUB02_003129"
     CDS             5314..5808
                     /locus_tag="OUB02_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8886861.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5905..6426
                     /locus_tag="OUB02_003130"
     CDS             5905..6426
                     /locus_tag="OUB02_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCT8886862.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6615..6926
                     /locus_tag="OUB02_003131"
     CDS             6615..6926
                     /locus_tag="OUB02_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCT8886863.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6954..7640
                     /locus_tag="OUB02_003132"
     CDS             6954..7640
                     /locus_tag="OUB02_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT8886864.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3083 a          756 c         1496 g         2359 t
ORIGIN      
        1 taaattaaat tttagtagga attttatagg tttattttta aaggtttttt gaaaataaat
       61 ctatttaaaa attaaattaa aatgcaagaa aaacataata actttcattt ttaagaagtt
      121 ttcacaaaaa agaaaaaata tagtgcaata aatgatacaa agtaatataa tagattacaa
      181 gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa aatgaataaa aaataaaaaa
      241 ataatcataa gttagaggta tcaatgaaaa ataagactta atgtttttaa aattaaaagt
      301 atactaaggg gggcattaat atgtgcgtta tatcaaaaga tagagcaaag gaaataaaaa
      361 tagtagatac tactttaaga gatggagagc aaacagctgg ggttgtattt gcaaatagag
      421 aaaaaataat gattgctgaa atgttaagtg acctaggtgt tgaccaaatt gaagttggta
      481 tacctactat gggtggagat gaaaaaaatg taataaagca catatgtagt agaaatttaa
      541 aatcagatat aatggcttgg aatagagcag taataaaaga tgttgaagaa tctatttctt
      601 gtggtgttga tgcagtagct atatcaatat cagtatctga cattcatata gaaaataaat
      661 tgagaacttc tagaggctgg gttttagaaa atatggctaa aacagttgaa tttgcaaaga
      721 aaaatggatt atatgtttca gtaaatggag aagatgcttc aagagcagat atagacttct
      781 taactgaatt tataaatgtt ggaaaacaag caggggcaga tagatttaga tactgtgata
      841 cagtaggagt tatgaatcct ttttctataa agaatgcaat agaaactctt tatgaaagaa
      901 ctaattttga tatagaaatg catacacata atgactttgg gatggcaact gcaaatgcac
      961 ttgcaggaat agcagcagga gcaaattatg ttggtgttac agtaaatgga ttaggtgaaa
     1021 gagcaggaaa tgcagcatta gaagaagttt taatggcact taagtgtgtt tataaatgtg
     1081 atttaaataa tatagatact agaaaattta gaggaatatg tgagtatgta gcacaggcat
     1141 caggaagaat acttcctaca tggaaaccag tagttgggga taatatgttt atacatgaat
     1201 ctggtataca tgcagatgga gctttaaaag acccacataa ctatgaacca tttgacccaa
     1261 gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca ttcaggaaga gctgcagttg
     1321 taaataagtt gagtgaatat gaaatgtata tttctccaga aaacgctacc aagctattaa
     1381 atgctataag agctacatct ataagattaa aaagaagttt aatggataaa gaaattcttc
     1441 aattatattg tgatatacta gcacatgaga aaggcacaac agaagaagaa gcagttagag
     1501 gttcatatat ttaagatata attgtttagg aggaaaatat gggagataat atagtttata
     1561 aaataattaa aaaacatata gtagatggag aagctgttgc tggaagttca attggtataa
     1621 aaatagacca aactttaaca caagattcta ctggaacaat gacatatctt caactagaag
     1681 ctatgggaat agataaagta aagactaaaa gaagtgtggc atttgtagac cataatatgt
     1741 tacaacaagg atttgaaaat gcagacgacc ataagtatat acaaacagtg gcagataaat
     1801 atggagttta tttctctaag ccaggaaatg gtatttgtca tcaagtattt ttagaaagat
     1861 tttcaacacc aggagataca cttttaggtt ctgatagcca cacacctact gctggaggtg
     1921 tgggcatgat ggctattgga gctggtggat tagatgttgc tttagccatg gcaggaggag
     1981 catactatat aaaagcacca aaagtctgta aggtgaatct agttggaaaa ttaaataata
     2041 tggtatcatc aaaagatata atattagaag tgttaagaaa acaaactgta aaaggtggag
     2101 taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag cctatctgtt ccacaaagag
     2161 ctacaataac aaatatgggg gctgaacttg gagcaactac ttcaatcttc ccaagtgatg
     2221 aaaaaacact agaatttttt aagagtcaag gaagagaaga ggcatggata gagctaaaac
     2281 cagatgcaga tgcagtttat gatgaagaga ttactataaa cttggatgaa ttaaaaccat
     2341 tagctgcaaa acctcatagc ccagacaatg tagatgaagt tgaaaatata ggcaagatta
     2401 aaatagacca agtggcaata ggaagttgta caaattcttc ttatgaagac ttaatgaaag
     2461 tagcacaaat cctgaaagga aataaagtac ataaggatgt gagtttggta atagcaccag
     2521 gttcaagaca agttatggag atgattgcta gaaatggggc tttggcagat ataataagtg
     2581 ctggagctag aatactagaa aattcttgtg gtccatgtat tggtatgggt caatctccag
     2641 gtactgactc tgtgtcttta agaactttca atagaaattt ctatggtaga agtgggactt
     2701 tgtcagcaca agtatattta gtaagtccag aagtggcagc agtaagtgca atcaagggag
     2761 tgcttacaga cccaagagaa tttgatatta aatttacaaa tttagatgta aatgaatttt
     2821 tgatagatga tagcatgata attaaacctg ctgatgtagg aagtgatgta gaagtagtta
     2881 gagggcctaa tataaaacct ttccctctta atacagagct aagtcagtct ataggtggaa
     2941 aagttatatt aaagacagaa gataatataa ctactgacca tataatgcca tcaaatgcta
     3001 agttacttcc ttttagaagt aatataccgt atcttgcaaa ctattgtttc aatactgtag
     3061 atacagaatt tcctcagaga gcaaaagaca ataatggtgg atttatagtt ggtggagata
     3121 attatggtca aggttcaagt agagaacatg cagctttagc accattatat ctaggtgtta
     3181 aaggggtaat agttaagagc tttgcaagaa tccacaaggc taacttgata aatagtggta
     3241 taattccaat ggagttttgt gatgagaaag attatgaaaa tatatctctt ttagataatc
     3301 tggagatacc aaatatttta gacaatctag gtagtggaat tttagaagtt aagaatacaa
     3361 ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc aaaagaagtt gatgtattaa
     3421 aagctggtgg aaaattaaac tacactaaaa atcaagctaa ttaatatgaa aagagggaat
     3481 attgaatgta taaagtgaca cttataccag gagatggaat aggaccagaa gttgcgaaag
     3541 ctatgaaaaa ggtagtagaa gctactggag tagaaataga atgggaagaa gttaatgcag
     3601 gagaggcagt tattgaagag tatggtactc cacttcctga atatattata gatagtataa
     3661 aaaagaacaa aatagctata aaaggcccaa ttactactcc tgtaggtaaa ggatttagga
     3721 gtgtaaatgt aactttaaga caggcattag atttgtatgt caatttaaga cctattaaaa
     3781 gttttaaggg gataaaatca agatatgaag atgttgattt agtggttgta agagaaaata
     3841 cagaagattt atatgctgga atagaacata aaataggtga ttatgcagct gaaagtataa
     3901 agataataac aagaagtgca agtgaaagaa ttgttgattt tgcatgtaac tatgttaaag
     3961 ataataagag aaaaaaagtt acagctatac ataaggcaaa tataatgaaa atgtctgatg
     4021 gattgttttt agatgtgttt agagaagttg catcaaaaca tggtgtagaa tatgatgatt
     4081 tgattgtaga tgcagcagct atgaacttag ttttaaatcc agaaaattat gatgtaatgg
     4141 taatgccaaa tctttatgga gatatactat cagatttagg tgctgggctt gttggaggtt
     4201 taggtataat tccaagtgca aatattggta aagattgtgc gatatttgaa gctgttcatg
     4261 gaagtgctcc acaaatagca ggtcaaaata aggttaatcc aacagcactt attcaatcat
     4321 cagtaatgat gcttagatac ttaggtgaat atgaaaatgc acaaaaaata gaaacagctt
     4381 tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga tttgggtggc tcagcttcta
     4441 ctacagagtt tgcggatgag gtgtgtaagt atattgtata gattttattt agggaaaaag
     4501 taaaatatag attgaaggaa agaggctttc tcaaaatgga gataagcctt ttttattgga
     4561 atgataaaat atatttgatt ttatacaaaa gagagttcct cataaacgaa aaagttaatt
     4621 ttgggatatc cattttaatt ttaaaattaa ttgattaata tgcaaagaaa gattaaataa
     4681 atttattatt tttaattgaa ctttcaacca aaatatggta aaatttaaaa aagttgaact
     4741 ttcaactaaa aatgaatata ttgattatgt gaaattctat aaaacagaaa ctatttgact
     4801 ttagccatag tagtttcagt ggaggtgtgt ttgattaaga ctttagacag taatatatta
     4861 agagaagtag gtactttatc tagagcagta aattcaataa atgatattaa gtacaaggaa
     4921 ttaaagttac aaaaaggaca atttacattt ttgacaagaa tctgtgaaaa tcctggtata
     4981 aatcttgtgg aattatcaaa tatgttaaag gtagacaagg ctacaacaac caaggctata
     5041 caaaaattaa taaaggcagg atatgtagat aaaaagcagg atgagtttga taaaagagga
     5101 tataatttaa ctccaactga taaatctttg gaagtttatg agttgataat tgaagaagag
     5161 aatagaagta tagagatatg ttttgataat tttacagatg aagaaaaaca agtggtaact
     5221 aagttattag aaaaaatgag caaaaacgta gaaaatgaat ggtttaaagt aaagagatag
     5281 taaataaata cattaactta aggagatatt tttatgatta gaaaagcgaa tatgaatgat
     5341 ttagaaagca ttatgaaaat tataaaatct acagtggaag aaatgaagac ttataataat
     5401 acacaatggg atgaaaacta tccattagaa aaagactttg tttcagatat taagaaacaa
     5461 gatttatata tttatgaagt ggatggagaa gtagcagggt ttatatgtct taattatgaa
     5521 gagccagaag aatatttagg cttaaattgg tcatctaata aaaaagcaat ggtaatacat
     5581 agaatggctg taaatccaaa ctttagaaag acaggaatag catctaagtt agttgatttt
     5641 gctgaaaaat tagctgtaga aaacaatgta tcatacttaa agagcgatac atattcaata
     5701 aactcaaaaa tgaatttatt acttacaaag tgtggtttta taaaaatcgg agaaatgagt
     5761 tttttaggaa aggagaaatc tttctactgt tatgataaaa tattataaag agcatttata
     5821 atacaagggg taaataatag attttcagca ttagtaaggg cataatataa cctagtatat
     5881 gctattatga aagggatgat ttatatgatt ggatttctaa agaaaagaag aagtattaga
     5941 aaatacaagg atgttgaagt agaaaaagaa aaattagata aaatattaaa agctgctctt
     6001 ttagctccat catcaaaggg gcttagaaca tgggaattta tagtggtaga tgataaagaa
     6061 aagctaatta atttatctca atgtagaact aaaggtggtg gatttttctt aaaaaatgca
     6121 cctttagcta ttgtaattat tgcagataaa gaaaaaaatg atgtttggat agaggatgca
     6181 agtatagctg cttcatacat acaacttcaa gctcatgaac taggtttagg ttcttgttgg
     6241 atacaagtaa gaaatagaat gcatgatgat aatatagaag ctgataagta cattagagaa
     6301 gaattaaaag tacctagcaa gtacagtgta gaatgtataa tttctatagg atatcctgat
     6361 gaagaaaaga aagcatacag tgactctgat ttagactata aaaaggttca ttttaacaac
     6421 ttttagtata taatatgttg tattaagaca ttatattttt ttctattgtt aatatatact
     6481 taaaattgta aaaatatatc tgaattttac taacttatct aggggttatg tacaaggatt
     6541 ttaaagataa atatagaaat taatgtttat aatcgataca taacttgagt aacaaaattt
     6601 aaggaggtta aagtatgggt atatttaaaa caaaaatgga tgaggactgg aaagtaaact
     6661 acattaaaga gtttaatgaa atgagagatt cttatgagag taaacttcaa aaaaaacagt
     6721 ttgaggtaga tagtttgaaa tcagaattgg atagactaag aagctataaa aatagcctta
     6781 agccaaaaga aaaacaaatt actgatgatg atataaataa tataaagagt ttaagaagag
     6841 atggcttaag ctataaagaa atttcaaatc aaacaagttg gagcaaagca actgtaagta
     6901 gggtattaaa tggactttac gattagcata tacatggggt gatttaaatt gggatgaagt
     6961 atagttacaa aaaatgcata atagattcta ttttattgat gtttatagtt caaatattaa
     7021 ggatgatttt gaattatgta cttctaagtc aatttgaatt tactcttgaa aattttaata
     7081 ttataaactt aatttctttt acattagttg gtttatcgtt aattttattt ttaaaagata
     7141 attctttgta taacaaggtt agaaatagaa aaatcacaga agcttttgaa gaaaataaaa
     7201 acaatatact tattgaaaag tgtaagctga tactttttgt tgtggtttta tctttagcaa
     7261 taattgtaac ttattgtact aagggatatg tactttttaa tgtaactatg atgactttgt
     7321 ctgtattgat agttccaata tttgaagagt tattttttag agaatatata tggaactatc
     7381 taagtaattt tataaagtca aagagtaaaa ttatctgtat aacatctata ctatctggaa
     7441 tttataatat aggatacata gatgttataa gaaattatgt aatattgtat aacaattcga
     7501 gctacacctt tgaagttatt atttcaaaaa ttatgatagg tactgttttt ggtattgtac
     7561 ttggattagt taagtatagg tttagagatg tagggttttg tatattattg aggagcttat
     7621 ttgctatatt tattaggtag atagtataat cggtttataa ggttgtttta aatttaattt
     7681 taaatcaacc ttat
//
LOCUS       DAKBNN010000181         7684 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000181 DAKBNN010000000
VERSION     DAKBNN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.182"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            73..273
                     /locus_tag="OUB02_003133"
     CDS             73..273
                     /locus_tag="OUB02_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886865.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(480..1070)
                     /locus_tag="OUB02_003134"
     CDS             complement(480..1070)
                     /locus_tag="OUB02_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HCT8886866.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1073..1948)
                     /gene="dpsA"
                     /locus_tag="OUB02_003135"
     CDS             complement(1073..1948)
                     /gene="dpsA"
                     /locus_tag="OUB02_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HCT8886867.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYTLDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(2024..3415)
                     /locus_tag="OUB02_003136"
     CDS             complement(2024..3415)
                     /locus_tag="OUB02_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCT8886868.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENLLSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3419..4555)
                     /locus_tag="OUB02_003137"
     CDS             complement(3419..4555)
                     /locus_tag="OUB02_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCT8886869.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEETKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4587..5132)
                     /locus_tag="OUB02_003138"
     CDS             complement(4587..5132)
                     /locus_tag="OUB02_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCT8886870.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            5456..6235
                     /locus_tag="OUB02_003139"
     CDS             5456..6235
                     /locus_tag="OUB02_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886871.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            complement(6690..7118)
                     /locus_tag="OUB02_003140"
     CDS             complement(6690..7118)
                     /locus_tag="OUB02_003140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCT8886872.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     repeat_region   7307..7659
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7373..7401
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
BASE COUNT         2648 a         1160 c          890 g         2986 t
ORIGIN      
        1 ttatatacta tgaaataaaa tatatttaat attatataga taaatttgaa gtttataaga
       61 aggaggataa gcatgaaaaa attaaaaaca attagcattt tttctttgat tataagtgta
      121 attttaacga ttggaggtat tggaatagta acttattatg tagataattt gtttattaga
      181 ggactatcgg tatttgtttt aattatgtcc tcttcttttg tatcaacaac agttagattg
      241 atatttgaag agtcaaaacg atataaattc taatttaaaa attggattaa gtaaataatt
      301 ttaatatacc aaaaattacg gagattagct caaaatagac tttttagtcc atgaggcatt
      361 tttttgtatg aaattaaata tattttcatc atttcaacaa gaaaaagagg cttctctcta
      421 ttttgagaca gcctctttgc tagtattgta tctaatacat aatattataa gaaccaattc
      481 tattgatgtg gactttttat aacaggctgt agctgtttgt tctctagagc aagttctata
      541 gttggaatta ttagctcagt atgtgcaatc atagaattag gctttgcttt gcaattatct
      601 tgaccaaaag gtacaaaata tatattttta gaattaagga gaatgccaat atttttgaaa
      661 ttaaagctta gagcatcatt cgttgatatt gatattacta gtggtttatc attacgaaga
      721 tgaccttttg ctgccattaa tactggtgaa tctgtaatac catttgctaa ttttgcagca
      781 gtatttccag tacaaggagc tattattata atatcagcaa ttcctttagg tccaaatggc
      841 tctgcctctt caatcgtaac tataggttta tttcctgtaa gttcatatgc ttttttcata
      901 aactcttcag aatttccaaa tctacaatcg atattttgtg atacatctga aaatatagtg
      961 tgtacattag ctccttcttt aaccaaattc tctaactcta taaatatttt atcataagta
     1021 caaaacgaac cagtaaatcc tactccaatt gtaagtcctt caagtctcat ttctaatccc
     1081 ttctttcatt tataatattt tctatagcgt taacaagaat ttcaccagat gtctttggtg
     1141 aatatttacc aggaagccca aggcaaagag tcgcattaat ccctgcttct ttaacaacag
     1201 aataatctac tcctccagga gaagaagcta tatctataat agttacttct tgtgatactt
     1261 tttctaacct ttctttcgtt aaaatacact ctggtatggt gttaaaaata taatcaaatt
     1321 gtaaaagttt ttcatcaagt tgacttaaag aaatactgga atatccaaat gcattagccg
     1381 tacttcttgc atcattattt cttgcgccaa cacatacatt tgcatccaaa gtgtataatt
     1441 tttttgcaag tatttgtgca catcttccat aacctaatac taaacagtta ctttgatgta
     1501 tattgattat acttctttta atagcttctg ctatagcccc ttcagctgta gcaactgcat
     1561 ttaaaatagc aacatcattc attttcataa aatcataaat ctgaatagag tgttgctcgc
     1621 aaaatttaca aactttagtt ggtatgttac ctccaaataa tatttgatta ggagataaat
     1681 taattagcaa gttatcaata gtagcatcaa gacaatcatc cgtattttca atattaactt
     1741 gattctttgt aaatggaatt ggacataaaa taatggaact tttggatagt gcttcagcta
     1801 gtgaagttga ttttccaacg attccattta taatttcatt tgatatccca taagtgacta
     1861 cagaataccc tttattaatt agttttttta ccatgtacac ttgtcgcagg tctcccccaa
     1921 taacagttat atcgtattga tttgacatat tgtaagcctc ctttactata tacaatatgt
     1981 ttttatcaat acattggttc actaaaatta ctaaatatta tacttataac atttttaata
     2041 atgctaatct atctatcttt tcagaagagg tatatggaaa tgaatttaaa aagataattt
     2101 tctttgggat ttcatttttc attagattat ctttcatttt aatcaacaaa tctttcttcg
     2161 taattttgtc tatagttatt acaaataata caatttgatt tcctcttata gtgtctgagt
     2221 acggcaatac aattgcatct tcaatttgag gaattttttt aacttcattt tcaatcttag
     2281 tagaactaac tttaaatcct ccaatattta aaatgtcatc acttcttcct tcaaatatta
     2341 agtatccatc attatcataa tatccaatat cattgacaga ataaggtttg gttgcattat
     2401 atactgcata ttctgtattc acaaaaattt ttccttctct aatatatata tctattcctg
     2461 gaaatggtct gcctacactt agaggttttt caattagttc atcatagcat aaatatgtaa
     2521 tataatttaa ttcacttgcc ccatagtata aaataatttc agactttggc atatattttt
     2581 ttagatttct tgcagtatct tcaaataata attgagaccc tgtaaaaata cttacaactt
     2641 tgagtactgg ttcttttaag tgtttaacca aaagttgaag ttttgtaggt ataaggtata
     2701 tgttagttac attatattgt tttattgtcc ttatccatga tttacaatta agacctgaac
     2761 ttattacaat actacctcct tcatataata ctgacattat agaatttaga ttccctgtaa
     2821 aactaagtgt tccattaaca aacaagattg attccccaga gatattaaaa ataccatttt
     2881 gtataggaaa aaatccagcc caactctcat atgttctata taataccttt ggaacacttg
     2941 tagaacctga agataaagct cccatacaaa tacttttatc taaataattt ataagcttct
     3001 tatattgata catatatata ttaaatttag gattaaaaat agaaaaatta actttttttg
     3061 ctacttcatt attgttatca tcatataaat caataacgtc catagattca tcagatataa
     3121 tgattgaaat attatttttt aataaaatat catctaatac ttttttagaa agattataat
     3181 gacaaataat aggaatattt tttgaataat taatagcaaa aaaactaatc aactgaaaca
     3241 ttatattttt agaatatatt aaaattggga tattttcacc taaagaaatt tgttttccta
     3301 gttcctcact ataactcaat agattttcat aagtatattt ttctccatct ataattaaaa
     3361 aatttttatt tttatgcctc tcttttttta ttcttaaaag ttcataatag ttcataatct
     3421 acaccctttc aagcagtaaa gctgaaccta gaccaccagc tgcagctata gagcaaattc
     3481 catatctacc cttagtctct tctaatgctt ttaaaagatg taatgcaata attgcacctg
     3541 aagcaccata tggatgtcca tacgctaaag ctccaccaaa tatattatat ctatctatta
     3601 actctggata ctttctttga aacaacacat ctataactgc aaatgcttca ttaaattcaa
     3661 cagcagatac atctaaataa ttaaggcttt ctatctctaa aagtttatcc attgcttgta
     3721 tcgctgatgt tggacttaag gttgcatctg ttcctatcgt gcatgtgtta ataatttttg
     3781 ctttaggctt cttttttaca tgttctaaat acttcttaga acatattata ataaatgaag
     3841 ctccatcatt aataagacat gaatttgctg catttgtaat agtttctttt cctaaaattg
     3901 atggcatcct atctaataac ctttgactca tcttatcacg tataccttca tcataggtac
     3961 tattatttat aggagatata atatcttcaa gtattttttc ctctcttgct tcttttgctc
     4021 ttttatgact ttcctttacc caaaaatcca agtctgcttt ctcaatttga tataactctg
     4081 ctactctttc cgcaccttcg agcatactat tttggctatt atcatcagga gaaaattgag
     4141 caactgtata atttggatta tttgtattat atcgcttatc attcttatga tatgtgcgca
     4201 ttggttgtag agaactactt tcaaaaccac ctgcaataat taaatcacac tgaccagatt
     4261 taacctttga aaaagctatg tctatactca tcatagcaga agcacactgc atatccacag
     4321 taaaagcagg tacttcattt gacactcctg ctgttaaagt cattaatcta gctatattcc
     4381 ctcctgtccc aactgcattt ccacatataa tctcatcaat tttgtcaatt tcatactttt
     4441 taattaaatc ttttaaaaca cttgctccta gtaattcagg ttgaacaaat tgaaaaattc
     4501 cattttttag acctatatga cttctcaatc ctcctaatat aaaaacttcc ttcatacatt
     4561 acacccttcc ttatattata gatttatcat agatttcttc tgagtacatt gttcagttta
     4621 gtgcctaaaa aagatgcaat aactgacttt attgtatctc caattaaaaa tggtactact
     4681 gctgcaaaaa gtgctgcttc tactgtcatt tgattaaaat agcacataaa tactgttccc
     4741 ataaaatata taataggcat tccaacaaaa attgttatta gtatatatct cttaatatca
     4801 ttgtttcgtc cttttaataa actaataatt agtgctgcaa acaaaaaccc aaaataaaaa
     4861 ccacctgttg ggcttaacaa ctttccaata ccagatgtac ctccaccaaa tactggaatc
     4921 ccaattaaac ctatcattat atatacacca actgtactaa atgcttgtct tggtgttaaa
     4981 atcaaagcta ttaaatttat cattattgtt tgtgctgtta ctcctgctgg agtaaatggc
     5041 aatggtatat atatgtaaga tgatatacac aataatgcta cacatattga cattttagtt
     5101 aaatcttgaa tatttacttt agtatttttc atttttataa ttcccttcat atgtcttatt
     5161 tcatttagtt tatatgtaca tatacaaagc ttctattaaa gctttgtttt agtataaaat
     5221 aaagacgttt ttttgtcaac tattataagt atttaggttt acatttaatt tataagtaaa
     5281 ctttatactg ttactttata ctttaatagt catttataaa actttgctta aaaacagctc
     5341 tatttagact atacttgtaa attataatat gatatatttt tatataagat tttattttat
     5401 ataaaaaata gtaacaaata gtttatacac aagatattta aaaggaagtg atgatatgaa
     5461 agttaatgaa aataatataa ataatataga tgaagagtta gatgaactta gtaggaaagt
     5521 ttacttagag tcaccagatt tttggataag tatttctgac aaaataatag ataaagtatt
     5581 tgatgaaatg ttattatttt ttgcagttaa atataactat ttcaatatag tatgctttgc
     5641 tattgaaaac aattatctgg atttaaacat gccttcaaaa aacaaaaaat acactaatat
     5701 aatggagcat ttgttagata catcaaaaca gaatgaagat aaaaaaattt ataattatct
     5761 tctaagctta aataaaggta ataacctaga tgaaagacca attttagatg aattaaactg
     5821 taatattagt agtgatactt ttgaaaaaaa agatatatat cttccaaagt acttatgtcc
     5881 aagttgtaat tctaatatat ttgatacagg ttataaagta tcaaatgaca taacatataa
     5941 attttcttct aaagaagctg aacctattga aatgtgtagt gaagtttctt ctatcagatg
     6001 ttgtaattgt gatgaattat tagaagatat tacacttgat aaactatata acctatgcaa
     6061 aattcaaaac tgtaataatt gtggttctaa tttaactaag gtaggaatta ttgcaaagaa
     6121 aaaaatgaac tttgatgaga atactgaaaa atttaaagat gtctcaacat cttactgttg
     6181 tggaaattgt gactctgaaa taaacttatc tcaaagagaa cattttaaga tttaaatatt
     6241 tataggctaa agtaataaga ttacattatt agatactgta taagtttttt aaatctgtaa
     6301 aaataaatac acttatacga attaaaattg ctatgaaact agttcttgat gaactaattt
     6361 cacggcaatt ttctataatc taataattat taaattttaa caaaccttta ttagcattcc
     6421 ataattattt aaaattcttt caaataccct atagtttata ggtgaaagta tctttaatat
     6481 tatatcaaac ttattatctt taaatagtaa atttgttaaa tctgaaatac accatacaat
     6541 tttattttcc tcttttgtag ctatatcaat tctgattaac ttacatctat aataaatatc
     6601 tttatcttca tatagtctat tttaaaaatt actcttaaaa aatcaaatgg caaataaatt
     6661 aattatcctt ttaatagaat gtaatcatat tatttactat attccattat gtactcttta
     6721 aatcctgaat cttcatttac ttgttctttt actatattaa aacctttttt attataaaac
     6781 acaactgctt ttttattatc tttatatact gctaagctta gacttttata ttttttagtt
     6841 gcataatcta aaagtttacc tccgatacct agattttggt atttgggctt tataaataaa
     6901 gctcctataa agcttttatc tattatgctt ataaatcctt ttatttcatc tccatcatca
     6961 tatacaaatg tatctgatat aggtatatat tcgtttttaa cagtattata gttattttgc
     7021 cagtattctt tacttataaa gtcatgtgct ttgattgtac ttttttccca tatttccatg
     7081 attttattta tgtctttatt gtttaatttc tttatcataa attttatttc acctcatttt
     7141 tgtattgatt ttataatgtt tataggtaag tgtattttat ttagatatga tttagttaat
     7201 ataaggtgga tgttttagta tatgctattg ttgattctac aattttatat actgctttat
     7261 taatataaaa taagtttcta aatactcgtt gatattgcta atattctact tatctaccct
     7321 tagttgatat aaaacctgac actgtgcctt gtgtgagtct tttctttatc ctatttacat
     7381 accacttagt taatataaaa cgttgttaga taaatcatat gctttagttg ctttagattt
     7441 acataccact tagttaatat aaaacacttc gaaattgcag ttttcagcat gttattgcgc
     7501 aatttacata ccacttagtt aatataaaac aaattcgagg tcttgatttt gaagtttttg
     7561 gttatgcatt tacataccac ttagttaata taaaacggtt tctaccaatc aaatcacgca
     7621 taattataga atttacatac cacttagtta atataaaaca atcattttgt atgattgttc
     7681 catt
//
LOCUS       DAKBNN010000182         7650 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000182 DAKBNN010000000
VERSION     DAKBNN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.183"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            260..1561
                     /locus_tag="OUB02_003141"
     CDS             260..1561
                     /locus_tag="OUB02_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8886873.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1637..1912
                     /locus_tag="OUB02_003142"
     CDS             1637..1912
                     /locus_tag="OUB02_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT8886874.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3245..3475
                     /locus_tag="OUB02_003143"
     CDS             3245..3475
                     /locus_tag="OUB02_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8886875.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3468..4049
                     /locus_tag="OUB02_003144"
     CDS             3468..4049
                     /locus_tag="OUB02_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8886876.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4627..5304)
                     /locus_tag="OUB02_003145"
     CDS             complement(4627..5304)
                     /locus_tag="OUB02_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT8886877.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAIQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5493..6857)
                     /locus_tag="OUB02_003146"
     CDS             complement(5493..6857)
                     /locus_tag="OUB02_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8886878.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLIVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6985..7506
                     /locus_tag="OUB02_003147"
     CDS             6985..7506
                     /locus_tag="OUB02_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCT8886879.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2979 a          866 c         1319 g         2486 t
ORIGIN      
        1 ttatattgaa caatatatta ataattggta gtaaagtata tcaaataaga tattgctatt
       61 atgtagtaaa ttatataaaa caatgtatca gaataaatag tatattaata atttgtagta
      121 tatcatgacg aataatacag tcataacaaa tagtgagtca taacaaataa tgtatttata
      181 attgataatg tatttataat tgataatgta tttaaaaatc aaaatataaa aacttataag
      241 gaaagagaga ttaatttata tgggtaaatc aaaagatgtt gaaatagaga agtctatttt
      301 aggcacaatt cttttagaca ataaattaag ctataagcta gatgaattaa atgaaaacat
      361 gtttatgaat gatatatgtc ttgagatatt taaaattatg aaagaactta aaaaagaaaa
      421 catagtgatt gatgtggcca ctgtgaaatc aaagatagat agaaaatctc tagcaataaa
      481 aacaagtgat gtgactaatc taattacttg gggtcagaat tttggtcttg atggtcatat
      541 taaaatttta aaagaaaatt tagctagaag aagcataaat caaaattgcc aaaacctttt
      601 acacagccta aaccttggag aaaatatcga tacttgtatt tacaaatttg aaagtaatat
      661 aaaggagatt ttggacaagg atacttatga aaatgatgat gtaaatagta tagcagggaa
      721 ggtgcttgat tttttagaaa acaaaaaaga tattggattt aaatttggta taaaattact
      781 tgataccact ataggaggtc tttttaaagg agagctaacc acaatagcag ctaaatcggg
      841 ggttgggaag actgctttgg cacttcaaat aatgttaaat tcatttaagc aaggtaaaaa
      901 aactttattt ataagtagag aaatgaccag tgagcaagta tttatgagaa atatatgcag
      961 agtaactggt gtaagtacaa ggaatatgaa atctaaagaa atagatgaaa atgattggaa
     1021 acttattgta aatgctatag gtgatttaag cgaaaataac ttaatttata tcaatgataa
     1081 gatagataca attagtgcaa ttagaaagag gataaggcag gtaaagccag atttattaat
     1141 agtggattat gtgcaactcc ttacatctca gaaaagcatg gataagagag aaagggaagt
     1201 agctactttt tcgagagaac ttaaaaatat gactttagat tttaatatac ctgtaatcca
     1261 attaagtcag ttaaatgatg aaatgaaaga ttcaagacct tatggagata gacctatgag
     1321 agatagcaaa gccatctacc acgatagcaa caatgtggtt tatatacatc aattaaaggg
     1381 gagtgattat gaagaagcag tgagagatat tggagaaagt gaggaggcag ttagagcatc
     1441 agaatacagg ggaattaaga tggttgattt aatagttgcg aagtgtagag atggacagac
     1501 taggcataag catttttgtt actttgggga taagttacat tttcaagaac taaattatta
     1561 ggtcaataaa gtagtaataa caagcttatt aaaagtcggg gaatagcaaa tagattataa
     1621 aatatgaggt gaaaatatgt tggaattaac aaaaattata gaattacttg aagaacacaa
     1681 gatagaagta agtattccaa aggagttaca taagtatagt ttattggaat accttgaatt
     1741 gtgtcaacaa agtgggggaa catattttta tattaaaaca ctggaagggg ctttaaaaga
     1801 ggctagaaat gagcttatga ttaaattgtt taatgggaaa aattatatgg aattagcgag
     1861 gatgtttgaa atgagtgtta ttaatgtcag gcatattgtt agaaaaaaat agatattttt
     1921 ggagaataca agtgtgatat acttctgaaa tagaagttat tatagttgaa ataaaattgt
     1981 ggttaataaa ttaaagttgt gtataagttt aagattatag aggagtataa gtgtgattaa
     2041 cagcatgatt ttgtgaataa aattgaggat atatatgagt taaatgtgtg attaataagg
     2101 taaggttgtg tataagatta tgagtattta aaaggagggg ctataattaa tagtgtagag
     2161 ttgtgtgttt gcttaagatt atataataat ttaatagatt aaactatact aataaggtaa
     2221 aatcgtgtgt ttagttaaag cttattataa aatcttgata tgtgattaat gatgcaatat
     2281 gtgaataaag ttaagattat aatagattta acctgtggct aatagaataa aattgtgaat
     2341 aaagttaaga ttataatagg tttaatctgt gactaataga gtaagagtgt aaatagagat
     2401 aaacttcaat atgagcttaa cctatgatta atagagtaag agtgtaaata gagataaact
     2461 tcaatatggg tttaacttgt gattaataga gtagggtgta actaaagata aacttcaata
     2521 tgagtttaac ttgtgattaa tagagtaagg tgcaactaaa gataaacttc aatatgagct
     2581 taacctatga ttaatagagt aagagtgtaa ctaaagataa acttcaatat gggtttaact
     2641 tgtggttaat agagtaagag tgtaaataga gataaacttc aatatgggct taacttgtga
     2701 ttaatagagt aagagtgtaa ctaaagataa acttcaatat gagcttaacc tatgattaat
     2761 agagtaagaa tgtaaataga gataaacttc aatatgagtt taacttgtgg ctaatagagt
     2821 aagagtgtaa atagagataa acttcaatat gggtttaacc tgtggttaat agagtagggt
     2881 gtaactaaag ataaacttca atatgagttt aacttgtggc taatagaata agattgtgaa
     2941 taaagttata gttttagatg ggtattaacc tgttactaat agaatatgat taggtgtaaa
     3001 attatagagc tgtataagaa aatattatag atatattaaa tattagataa taaatattta
     3061 tatagataca caaaagaaaa ggcgatatga agctaaactt aatgacttca tatcgttttt
     3121 tatttgaaac tcctttgtat ttagagtgtg atttttaata ttgaggtttt atgtgattaa
     3181 gtcacagata aaaagtaagt atcttgatat aattcttata gattaataat atagggaggt
     3241 tattatgtct aataaaaaaa agaagtatgc aaatatttta gcaaatgagt gcgttgcttg
     3301 tggtagctgt ataaaagcat gtccaagaag tgctatctca gttccatgtg gcattagtgc
     3361 aaaaattaat agagatttat gtgttggatg tgggatttgt gaaaagatat gtccagcatc
     3421 tgtaattgag attataacaa tattaaaaga ggaagaaggt aagtgtcatg aataataaaa
     3481 aacattggta tgactattta tggatttttt cttcaatata tttgatattg ggatttgtaa
     3541 atattatttt cgcttggatt gggctaattt gtttctttgt tccactagct atttctattg
     3601 ttaagggaaa taaagcttac tgtaataagt attgtggaag agggcaactg tttaatttgc
     3661 ttggaaataa attgaagctg tcaagaaata gagatattcc taaatttatt agaagtaagt
     3721 ggtttagata tgggtttttg acatttttta tggtaatgtt tgctaatatg cttttttcaa
     3781 cttatttagt gtttgaagga agtagaaatt ttaaagaagt agtgactttg ttgtggacta
     3841 taaaaatgcc ttggcaatgg gcgtatcatg gaactttagt ttcaccttgg gtggtacagt
     3901 ttgcatttgg tttttatagt gttatgctta catcaactgt tcttggtttg attactatga
     3961 tattgtttaa acctcgttca tggtgtgttt attgccctat gggtactatg actcagatga
     4021 tatgtaaggc aaagagtaag tctgtataga aaggccttat gtgatattta cagttttgtt
     4081 caagtagaga tgagtagtag gtttgaatat atatgacaaa tatatgagta taggggcata
     4141 gatttagttt gtgtaaggca aactgattta tgcttctttt ttatgtctta gattattggc
     4201 taagtagaaa caatgaattt agatattaag aattattcag ttatttgaag aaaatagaat
     4261 aataattaac attatatgaa tggatattca ttacatataa gcagttaata gcaagattta
     4321 tttaatatga aataggtcaa gtatttaaac taaataaggt catgacaatt aatataagta
     4381 tagatataaa aagattagaa gttataactt ataaaaggta ttctatataa gtagtatgtg
     4441 gaaaatagtc aaattaactt attagaaaca aaaaatatac ataaaaaata aatgtaaata
     4501 tcaaataaga tactatataa aaatagatat aagtatcttt tttattattg aaattatgaa
     4561 aatatatttt attttacaca aaaaagagtg cctcatgaac taaaaagttc attttgagac
     4621 acacttttac agtgtcaaag aacgaagttt atccttatcc aatactttta taccaccacg
     4681 agataaagac acaatacctt ctctagcaaa atatttcaac attctcgata caacttctct
     4741 ggcactaccc atatacttag caatttgctc atgagtcata tttatagtgc tactgccatt
     4801 tttagctatc tcatcaatta aaaaagatgc caaacgctta tcaaaactca taaaaagaat
     4861 ttgttgtata gcccacataa catcagaaaa acgttcggta gctaatttat atgaaaataa
     4921 ctctgcatga atattttctg tagataattt tgcaaaaaca gaggaactaa tctgtataat
     4981 atcgcagtcc gtttcagcat ctacatagac atcaaaagtt attgttttta gaatacagga
     5041 agctgataaa atacatacat ctccatcatc aagtcggaac agtgtaacct ctcgaccttc
     5101 atctgaaagt atgtatgtac gcactgttcc agactttata atcaaaatac caatacagtc
     5161 actatcacca caatgtactg gattcccttt tttgtaatgc gaggtatttg ctgaagaaat
     5221 taataattct ttttgtaagt cagtaagttt atcccaaaaa ggcaagctat gtgtaagtat
     5281 atcaatttca ttttgtgaaa tcatacaata tctcctttct taaattgtta taatcccatg
     5341 atacaagtga atagataaat tgacaagtag tttgttaaga ggattattta gtcagatagt
     5401 aatagtatga tttatgtaga aaaaatatga taatatgtat tcttgctatt aatgagaaag
     5461 taatatgttt tgatgatagt aggtttgtat aactaatgca taccgtttat ttttaaaaga
     5521 ttaagttcct tatgtaacct tatagacata aaaccagcaa ttatagcagc catcaaatcg
     5581 gctattggtt gtgcatataa gataccattt aacccataga atacaggaat aatcaaaatt
     5641 atagggataa aacagacacc ttgtctacac atacctaaaa atccaccttc ctttgctttt
     5701 cctagtgcga ggaataatgt agaatacact gtatagaatc caaatagcat aaatgaaaac
     5761 ccatttgccc taagagcttt actaccattt tctattaata aaatgtcacc acttgtaaat
     5821 tgtgaaatta tagattttgg gaagagtatc aatataagac caagcactac acaaaatact
     5881 gtagtccata atatggaagt ttttattgct atatgtaatc tatcgtagtt ctttgaacca
     5941 aaattataac cagcaattgg ttggaaacct tttataaaac caaatacaat aagactacct
     6001 aaagatgtta ttcgtgttac agcacccatt ccagctatca cagaatcacc atactttgta
     6061 gcctccatat tagttaatgt aatggcaaga ctagtcaata attgaaatat taaggtaggt
     6121 atgccaattt ttaggatttc tgaaaaaatt tcttttgaaa aacagcattc gttaattcta
     6181 aaactaaata tacttttctt gcttaaaata tatcctacat aaacaagtgt tgaagcaatc
     6241 tgtgagatag ctgttgcaat agaagcaccc cttaccccca gaccaaaagt ataaatgaat
     6301 atcgggtcta aaatcatatt aagcaaagca cctaataaca tagcacacat agttgtttta
     6361 gcagctcctt cacttgtgac aatattagtc atagtgacat tgaatatatt aaaaatagat
     6421 gacacgacat aaatgctagc ataagatatt gcataaggca taatactttt tgtagctcct
     6481 aacaatttta aaattggatt gagaaaaatc ataatcacaa taataactaa tgtacccact
     6541 ataatactac tgtaaataga tgtacttacc acttttcctg ctttttcatt atcaccatgc
     6601 cctaaaagtc tggcaatata tgaagcagct ccatttccaa acaataatcc taatcccaca
     6661 acaagctgac ctaatggaaa tgcaacggat atagctccca tttgacttgt tccaagtcca
     6721 cccacaaagt atgcatcaac caaattgtat aatgcagtta tcatcatacc tatcatggta
     6781 ggtaatccca tagataacaa tgcttttgag attggggcag accctaataa ttcaattctt
     6841 ttgttacttt ctttcatact taacactccc cttctattga cattaagtct aaattataat
     6901 actataaatt atagtaagta tggaaaatag tactataatt tatagtattt gtcaatagta
     6961 agaaaactaa aggagatgat ttttatggct acaatagatt taattgtatt gggaatagta
     7021 aagcaacaac ccaagagtgc ttatgatatt caaaaggcag tggaatacag gaatatatca
     7081 cgttgggtta aaataagtac accatcaatt tataaaaaag taattcaatt agaacaaaag
     7141 ggatatattg aaagtaaaac tgtgaagaat ggaaaaatgc cagaaaaagc agtttattca
     7201 ttaactgaat taggtgagac acatttttta gatttaatgt ttgatacatc tagcaaacca
     7261 ataaatattc ttttagattt taatgctgtt atagtaaatt taaatagcct tacaccagaa
     7321 aaacagaaat tatgtctcaa taatattgaa gagaatgttc aagagttaaa gaaaactatt
     7381 gaggaaaata taagtataaa gacagaagta ccagagacag caaaagcagt gttggagcaa
     7441 cagttggtgc tagcacaagc attggaaacc tggataaatt ctttaaaaga taatatggga
     7501 aattagaatt agagaagcta acttaataat cattaaacaa aaaccagcat atttaataat
     7561 gagatattag aaaaaataaa acaaatgata taaactgaaa agtatattta aaataaagct
     7621 cacttaattg agggagaatg ttaaactttt
//
LOCUS       DAKBNN010000183         7635 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000183 DAKBNN010000000
VERSION     DAKBNN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.184"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            108..1511
                     /locus_tag="OUB02_003148"
     CDS             108..1511
                     /locus_tag="OUB02_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8886880.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1602..1842
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1885..2583
                     /locus_tag="OUB02_003149"
     CDS             1885..2583
                     /locus_tag="OUB02_003149"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCT8886881.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2606..3607
                     /gene="trpS"
                     /locus_tag="OUB02_003150"
     CDS             2606..3607
                     /gene="trpS"
                     /locus_tag="OUB02_003150"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCT8886882.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4259..5826)
                     /locus_tag="OUB02_003151"
                     /pseudo
     CDS             complement(4259..5826)
                     /locus_tag="OUB02_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5801..5944)
                     /locus_tag="OUB02_003152"
     CDS             complement(5801..5944)
                     /locus_tag="OUB02_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886883.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6162..6344)
                     /locus_tag="OUB02_003153"
     CDS             complement(6162..6344)
                     /locus_tag="OUB02_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886884.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(6740..7486)
                     /gene="cdtR"
                     /locus_tag="OUB02_003154"
     CDS             complement(6740..7486)
                     /gene="cdtR"
                     /locus_tag="OUB02_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HCT8886885.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2732 a          913 c         1115 g         2875 t
ORIGIN      
        1 gaatatgcaa atattttgaa aaatatcagt ttaataataa tgaatctatt ttattaattt
       61 tgaaattaaa tttaaaataa ttaatagaga ggggaaatta aattaggatg gaaaagaaaa
      121 aaataggcct atggagtttg gtatttatga atgtatcagc tttatatggt ataagatgga
      181 tagctaaatc tacagcatca agctttggtt taggattagg tgcaatacct atgtggttat
      241 tattctcatt tatatacttt gtaccaggag ctttaatttg tgctgaatta gcttctacat
      301 atcaaaaagg tgatggaggt ctttacgatt gggttaagga agcctttgga gaaaagtatg
      361 gatttttagt ttcttggtta aactggacag ctaaaatatt ttggtattct tcatttttaa
      421 ctttcttagc agtaaatgtt tcttatacaa taggaaatcc agcccttgct gataataaga
      481 tgtttgtact tatattatct ttaataatat tctgggcatt atctcttata gcaactaaag
      541 gaataagttt tacaaaaata tttactaata caggtgcatt aggttcaaca ataccaagtg
      601 ctatacttat aatattttca tttgtagcag tatttgtgtt gaaaaaacat gatatagctt
      661 ctacatacac aatagcttca atgactccaa aacttaatat ggatgcattt gttgctatat
      721 cagctataat gtttggttta gcaggagcag agactgctgc taacttcata acagaaatag
      781 ataagcctga aaagaacttc cctaaggcaa tattaatatc agctggaata gtagcttcat
      841 tatatgtatt aggttcaatt gcaataacaa tgataatacc accagaccaa attactgctt
      901 ctaaaggtgt attagatgca ctttctgctg tttgtgcgtc tttaggaatt ggttcttggt
      961 tggttcaatt aatagctttt ggtatagctt tttcagtatt aggagcaata gttctttaca
     1021 tagcatcacc aataaaaatg ttatttggta gtgttaaaaa aggaatattt ccagatagtt
     1081 tagtagaggt aaatgaacac aagatacctt ctaaagcagt tatacttcaa gcaataatag
     1141 ttactataat actattggtg actacattaa tgccttctgt tgatgctata tacaatgttc
     1201 ttgtaactat gacagcatta actgcattat tcccatatgt attgttatat gcttcttata
     1261 ttaaactaag aaaagagaga ccagatgaaa ttagacctta tactatggca aaatcaacaa
     1321 gtacttgcct aggtttagca aaaatggttt tagttgtaac tgtagtgggt atattattat
     1381 ctgcagcacc agttatgcct acatttgctg aaaatgttat atatgagata gagatgattg
     1441 gtggaggtct tctagttata ttatctggtc tatggttatg gaatagatat gaaaagaaaa
     1501 cagctaaata aaatgtattt taaaaatttc ttttggatat tataatatat agttgacaat
     1561 ttatagtgta aggaatatta taaaataaag aaattaataa attaaatcct gagataagaa
     1621 atagtaaata aatatatttt tgcacagaga attaaggatg ctgagaactt aataaaaagt
     1681 atttattgaa tgggtctttg aggagtaaaa tgaaatgtaa tttaagatta cagagtattt
     1741 gatatcgtgt atgcacgtta tagcatatat taagatatat atattcaatt ttgagtgtat
     1801 ataaatttag gtggtaccac ggaaaatata tccgtcctat tgatttagga cggatttttt
     1861 aatatagaaa atttaggagg aaaaatgaac gtaataggaa ttataggtgc aatggatgaa
     1921 gaagttagta ttttagttga tttaatggat attagagaga ctataaaaaa agctagctta
     1981 gaattttata agggaatttt ggaaggtaag aatgtagtat tagtaaagtg tggcataggt
     2041 aaggttaact ctgctctttg tgcccagata ctaataagcg aatttaaggt tgatgctatt
     2101 gttaatacag gtgttgcagg agctttaaac gaaaaacttg atgttaatga tatagtaata
     2161 tcaactgatg ctatacagta tgatgtagac actacagcat ttggagaccc taagggagtt
     2221 ataccaagaa tgaagacttc tgtatttaaa gcagatgaaa gacttataga tgctgcttac
     2281 aaatcttctg tggaagaagt aaagactcat aaagtattaa aaggaagagt agttacagga
     2341 gataaattta taaattctaa agaattaaaa gaagaattag taaatgattt tggtggatat
     2401 tgtggagaaa tggaaggtgg agctatagct catgtatgct atttaaataa tactccattt
     2461 gtaataataa gagcaatgtc tgataaggca gatggaagtg ctgatgttac ttatgatgta
     2521 tttgtacatg atgctgccaa taactctaaa gacatagttt taaatatgtt aaaatcaata
     2581 taaatatata aggaggtaca aaaaaatgag tgaaaaaaaa gttatattta gtggagcaca
     2641 gccatcaggc aaaatgactt taggaaatta tttaggagct ataaaaaact ggactgaact
     2701 tcaagacaat tatgattgtt attatagtat agttgattta catgcaataa cagtacctca
     2761 agaccctaaa gttttaagag ccaatactat agaattactt gctcaatata tagcttgtgg
     2821 attagaccca gaaaaaaata ctatatttat acaatcacat gtcaaagagc atgttgaact
     2881 tatgtgggta ttgaatacta tgacttatat gggtgaatta agtagaatga ctcaattcaa
     2941 agataaatca caaaagagtg aagcaaatct aaatgcaggt ttattcacat atccagttct
     3001 tatggctgct gacatattgt tgtatcaaac tgacctagtt ccagttggag atgaccaaaa
     3061 acaacattta gaattagctc gtgatttagc aaatagattt aataatagat tttcacctac
     3121 ttttgtagtt ccagaaggat actacccaaa aggtggagca agagtgatga gtttgcaaga
     3181 gccaactaaa aaaatgagta aatccgattc aaatgaaaat gcatttatac tattagcaga
     3241 tgattcagat tctataaaga gaaagataaa aagaagtgtt actgattctt taggtgttgt
     3301 aaaatacaat gatgaacaac caggtattaa aaatctaatt gatatatatt ctaatttaag
     3361 taaaaagagt gtagaagaga ttgtaaacat gtatgaagga aagggttatg gcatatttaa
     3421 agaagatgtg gctgaagtag tatcagaagc tttaagacct ataagagaaa aatatgttga
     3481 ccttcttaat aataaagatt atttagaaaa aatatatagt atgggagcag aaaaagccga
     3541 aaaacaagct agaaaaactc ttagaaaagt ttataaaaaa gttggattta tagagagaag
     3601 atactaatta ttagctatct ttacttgata aataaatttt aaagttatta aaaaggtata
     3661 tgatatgaaa gagagagtct caaagtgaac ttttgttcat gaggcactct tttttgtatg
     3721 aaataaaata tattttaatc atttcaacaa tgaaaaagag gattatctct attttgagac
     3781 agcatctttt gataataaaa aaagatgagc ttttgctcat ctcaaagggg ttagattatt
     3841 cttactttgg ggaaagtaga ataaaggtaa aagttaactg atgtcaactt tgtctttttt
     3901 gcatatcact actgagcata aaaaaatcag aaaaccatag ccttctgaaa caacttctaa
     3961 catacattgt attttatacc caatatgtaa tttatgagtt aattttaact cttataatga
     4021 aaattgtcaa taatatatca aaaaaatatt ttttaaattt atataattaa atgaatttat
     4081 gtttatatat aggtgatatg atacagataa ttatcttttt aatacaatat agttctaaaa
     4141 aagaatatat tagaagaaga taatatttaa ataaaaaata ccttataaag ttaatttttt
     4201 aatactttat aaggtaaata catattgtat tgtggggata aatttaaatc atttgatact
     4261 aatcaacact aagaaccaat aactctctat catcttgagt aattgtatat attcttaatt
     4321 tcttgtatgt cataatattt tctccacctg taaaataact cctaagatta acacaatcag
     4381 ttttaggttg atttgtatct cctaaataat tacgcaattc tttatcttta gtttcaatat
     4441 ctttaaatcc aaaatattgt aaagtagctt caactctata tttttggaca taatcgagag
     4501 cttctttatt agtttatgtt ggtgtaaaat acataccatt tatataagcc tttattgtac
     4561 ttggattaaa tttaaaatct gcagaatata ttttatgtgt aactattatt tcttgaacag
     4621 ttggaatttt aacttctggt tcatcaagta tttcaggagt actatttagc tctgtaatag
     4681 ataagttatc taagtatatt gtatcactat caattaatgc tatatttata ttagaagaat
     4741 ctttttcagt agtttcaaat tcataactaa attttgtata tccttgttct agtaccaaat
     4801 aatccgtttt ttcttctttt acttttatat ttacaattat tgaattatat gttaaaggat
     4861 tttttgaatt ttactaaaaa cataacgttt ataaggtttt agttcagaca taggtatttt
     4921 aatcttcgtc tcaccattta atctatttgc tgagcctttt aaaccatctt gatttgtagt
     4981 attaacatta ctccatatac tagggtaatt attgtaatca tcaaaattag taaagtaagt
     5041 tggtgttttt ataagtatat tcattcctct ttcaagttta acattacata tttgtttatc
     5101 agacaaagtt tttaaactgt ctttaatctt attgcctata ttatcatcaa atatgagttc
     5161 aacacaactt tcatctattg gtatattatt aaaatataac aaaccatctt ttttagtagt
     5221 gccaaaagtt ttttcaattg cttctccaat tgtaagttca ggtgttttat cttctgggac
     5281 ctgtaaactt ttagcagtaa ttcttctttt gtaagattca ttttctgtat ctaatataat
     5341 agatacagaa atactgtcaa tttgacctat atcgtcttac caactatttc attctgttac
     5401 tatttgtcca gaaccatttt ttgtaccaaa atttccactt acttgtgttg tttctaattt
     5461 aatttgcttt ccatcatcta atttttttaa ttaattataa tttataggaa tcagtctaga
     5521 actaaattga tacattgtgt taagagccat aggtgaaaat ccttttttga gataagtatt
     5581 tccaggagat agattattgc caatttgatt ttcttgtgct ttgatagttg ataatgcatc
     5641 tccatctaac actaaatttg ttgttggtgt cactttgtac ataggtgcag tacctgtgtt
     5701 gtagtatcta atatttgcat ttatatatgc agattctcct ttgtttatac ttaatccagt
     5761 atttcatgat tatccattgc tattttgaac agcagttgaa ttatctgttg tatggtaata
     5821 atttgtcttg tatagtttct ttaaactcat taaatttttc taatgaaatt tcgttttgat
     5881 tttttgttct tattacttta ttaaatgcaa atttttctgg agattcaaaa taatatacat
     5941 acataggttt atctatttta ttttttgata tagcatttcg aagttctcta tattcttttt
     6001 ctcaagaatt tgcttctatt tgataatcat aaaaataatt tcttgtctga gaatatttgc
     6061 ttatctctat agaatctttt ttatatgatc ctaatacttc tttttcagat ttttcaagtt
     6121 tttgatcttt tctttctcat tctttagcct tttctttatc tttaaaaaaa tccttctgat
     6181 ctttctatag gagccttgta agtagtatta caaacttttc cactatagct ttgtaaatct
     6241 tgtgcataaa tgttattagg taacaaacta cctaaagtta gatataatat caacaatata
     6301 gatatacaat tactaatact tttatgtttc ctaaattttt tcatttattc tccctacaaa
     6361 tattagttta gttaactttt gaattttaat ttatttagat tatataatct aaataagaat
     6421 tttttatata aatttcataa aatatgtagg tatatatcat aaaacataaa aatatgtatt
     6481 tatatgatat gtcagtacaa aataacaact gaatatttta aagagactat ttcgtacttt
     6541 tatgatttaa tgagctatct tatcgaatca taatctagga acaattgtta taatatctaa
     6601 tagtagttat atatgatata gtcagtatgt gttttttcac aatatgatta ttatatagag
     6661 gattcttagg gattactctt tttgtataaa aaagagatgg ttcaaatttt tatcttgaga
     6721 catctccttt ttctatttat tatgttttaa taatattctt taaaatattt ctatatgtat
     6781 tagatatacg acatatatgg ccattactca ttttaatatt atttttcttt aggtcgagag
     6841 aaacaatatg atatttatta actacaaaag atttatgtac acggataaag catgcatcta
     6901 aatctggtaa agactttaaa cttttataga attcaaatat tctagaagta gtcactaatt
     6961 ttattttatg tggaatagtg cttgtttcaa aatatataac ttcatcaaat gaaattttcc
     7021 atagtgagaa atctgaatag atagtaaaaa aattagagcg ataatttttt tctttaatgt
     7081 atcttttgtg agcaatttta atacaagctt ttattttact ttcagcaatt tccatatcat
     7141 gttttagtat aaagtccata acttctaacc tataatcgaa gatccctaaa atcatttcta
     7201 tatgatttgt aagaaatatg attggactaa tataatctga ttctctaatt ttttgtgcta
     7261 tccataatcc atcacataat tcattactta tcttatattt tgcgtctaca actataaaat
     7321 atattcttac tttatttttt tcagtaagct cttttaataa caaatttgca ttatgatata
     7381 atcttacaac atcattatca aaaccttctt ttattaaaat attatttatt ttttctttta
     7441 gtttcattcc aaaacaaaca tcattatcaa agattaatat atccactaaa taccctccta
     7501 taaaaaattc aaaagtaaaa aaacaaaagt attttataga aaatacataa aataacacta
     7561 ggagataaat aagattaagg atagttagaa ttaaggtata tttatgcttg tttatatagt
     7621 atatcttata cttat
//
LOCUS       DAKBNN010000184         7624 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000184 DAKBNN010000000
VERSION     DAKBNN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.185"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            75..410
                     /locus_tag="OUB02_003155"
     CDS             75..410
                     /locus_tag="OUB02_003155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886886.1"
                     /translation="MDEKKLWQKISGSITFYLKDYDRSKSNEELLEDYLDYVLPDDIE
                     DDGVRRYLDKQTFKYVEVNKELEEKAISAFIERLNKRRSKEEPIEKVEEKRVYKNNVF
                     SFDEYKNRK"
     gene            1031..1507
                     /locus_tag="OUB02_003156"
     CDS             1031..1507
                     /locus_tag="OUB02_003156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886887.1"
                     /translation="MEKEEFNKLKIKDQVEYINQKLDKKSLTEICKDINISRATIRKR
                     FLKQGYEFNKIQNKYICYLELNNKEEEENKCNNNTKVLEEKIQVLESKIETIENKLNN
                     YNSRNIKKFEGKTVSRCYRLYEEVQKEFSKFCKENSNYKVQDILSMALYEYMKNRK"
     gene            1732..2301
                     /locus_tag="OUB02_003157"
     CDS             1732..2301
                     /locus_tag="OUB02_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886888.1"
                     /translation="MNKFELFKKVIVGNFNNLEQVEKEKSEGKVIHPIAKHVNRICND
                     KIKNLPKEFKGIFVIEESYYTDIKTDRTNILPHLFLFEEVENGQVKLTSYEIPKDISK
                     KDFTNSNEELKMDYNELEVSTKFMPMIYEYKEDKGFYGKSLSDFGRGMTFLLEETLSE
                     NEFEVNEILKRGDQVLVGFETPIIYRREI"
     gene            complement(2833..3183)
                     /locus_tag="OUB02_003158"
     CDS             complement(2833..3183)
                     /locus_tag="OUB02_003158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886889.1"
                     /translation="MFTSKEQLLYDSISEIAEHIGHDIKNPISLSLLCLDLGISFDDK
                     GKIFVAFNKIIRNNNFDDLNLQLFKNELISIIPTAKDYSDIVIVGLIKAFSKNLIPEL
                     YPYSNVVDLELDSE"
     gene            complement(3196..3666)
                     /locus_tag="OUB02_003159"
     CDS             complement(3196..3666)
                     /locus_tag="OUB02_003159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886890.1"
                     /translation="MKFLKKSKFVSIILSGAILITAIMPINTFAMESEQLKSAYISNE
                     VLPLPKESDYSNGSQTRNLATKICKEAIEFIIKHPNQSKNVVEKVAGKTVAKNFAKHY
                     SKICKGLKPLLKWSEIPYQALYDATFRVLVNSGISRSVASNIALAIKEGASWFF"
     gene            complement(3895..4122)
                     /locus_tag="OUB02_003160"
     CDS             complement(3895..4122)
                     /locus_tag="OUB02_003160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886891.1"
                     /translation="MFFLENRKNYKPNSNKKTLSKTNDKNPKVKTRFHNISERYNNYS
                     DDEFEEVLNNSQQDKFTNTNSEQNIDMKAIR"
     gene            complement(4353..4610)
                     /locus_tag="OUB02_003161"
     CDS             complement(4353..4610)
                     /locus_tag="OUB02_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886892.1"
                     /translation="MLEQNKKLLDIEEKIKQLKAQKQAIINKEKEKERKARTKRLIEI
                     GAIMDSMGIDSIDLANEFKNYFNNNEKSKLWLESFIEKNKA"
     gene            4863..5975
                     /locus_tag="OUB02_003162"
     CDS             4863..5975
                     /locus_tag="OUB02_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCT8886893.1"
                     /translation="MAIYHLHTKIISRSKGKSSVGASAYRSSEKLYNERDGLEHDYTR
                     KKGVVYKEILAPQNAPKWATHREKLWNEVEKVEKSKNSQLAREIDVALPIELSLEKQI
                     NLLRDYVQDIFVNKGMVADFVIHDKKDGNPHAHIMLTMRPFDESGEWGVKAKKEYILD
                     KQGNKIYDKKGNAKSRKIETTNWNKKETLEHWREQWAFYTNRILEKENIKEKIDHRSY
                     KDREIEKVPMKHEGVAVRAMESRGINTEIGDLNREIKTLNRQLELIDKQINAQEGIKE
                     VLYGFKDNGARNEDNRVRIRDNEMEQGYRGYSVGTNVTDGSAVEKLKQLAREKKERDR
                     EYREGIERENRKRLEQERQKSNIKTVRRSRNSEMEL"
     gene            5905..6288
                     /locus_tag="OUB02_003163"
     CDS             5905..6288
                     /locus_tag="OUB02_003163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886894.1"
                     /translation="MSKSDKNQILKQLEEVEIARWNYNREIKEIKSDIRDLKECNNDI
                     NRKYEEIKESIGLIKNKDKEMKKKRRVVIGIIIWLVISLYMNLLDDIEGIKNKIIREH
                     GYGMTIQDNKEIMAFIQKKGNKYKI"
     gene            6328..7572
                     /locus_tag="OUB02_003164"
     CDS             6328..7572
                     /locus_tag="OUB02_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCT8886895.1"
                     /translation="MRNTLIRVDDIDESIEYIDTIHGESKGWITKAEINKNKEFSQWH
                     YLVGDLLKQDFDKENIYISMSTFYKPMRRIETIKEIGSLFIDLDTYNTQFTKTQILMN
                     LEENYFNRSIPIPNLIIDSGRGLTLVWSIEKVPHKALPLWKAVQEYLYSQLKEFGADR
                     KALDVTRILRVPGSINSKSGTRVTILEKYEYKYTLREIQSEFLPDLDENRPKKKGRPK
                     KVVYIHRERSLYQGRILDLVKLCELRNYDVKGQREIILFLYRYYLCYFYEDEQKALED
                     VLELNKEFIQPLSEKELIMATNSAEKVFKSKDKQYKYKNETLIELLEISEYEQIHMKI
                     IIGKEEYKRRNNEYNKKKYKEKLKQLGKMTKQEELNILREKIKVLREKGLKNSDIASK
                     LNIPLKTLKRHITYMKKNDLLA"
BASE COUNT         3266 a          723 c         1152 g         2483 t
ORIGIN      
        1 aaaaaaaaag gtctcatttt ttttcagctt gtattatagg gtacgtagta cctgttacta
       61 gaaagggtgt ttttatggat gaaaaaaagc tatggcaaaa gataagtggc agcattactt
      121 tttatttaaa agattatgat agaagtaaaa gtaatgaaga actcttagag gattatttag
      181 attatgtttt accagatgat atagaagatg atggtgtacg taggtattta gataagcaaa
      241 catttaagta tgtagaagta aataaagaat tagaagaaaa agcaataagt gcatttatag
      301 aaagattaaa caagagaaga tctaaggaag aaccaataga aaaagttgaa gaaaaaagag
      361 tttataaaaa caatgtattt agttttgatg agtacaaaaa taggaaataa atttagatta
      421 aaacaataaa aaaattatag aatttataaa aataaaaatc agttttacat taaaaatatc
      481 tttatgtaag attgattttt ttatttataa caaataaaaa atagatgaaa caacggacgt
      541 tttattttat cttgaagtga tttttcttca atggaaaata aataaggagg gcgttagcaa
      601 caagccactt cagtggtgcg ttagtaaata aaatttattg ttaaaaattt attttatatc
      661 aaaatctaac taaaaagaat gtgataaaaa gtgtgattta gtgtgataat tttgggacat
      721 aagtgtgata aaaggtgtga taatcgtatt ttttttgtac ccaagttaat ttattaactt
      781 ttagagtatt gctaggcttt gcctagctca ccattagaaa tggtgacaaa aactccttat
      841 gctctttttt aaaaaaacaa gtttttaaaa tttagtaagt ttttcgctca ctcaaaactt
      901 atttttaaaa taaattttca taaaaaataa ttcaaaataa atgcaaaagt attttatttt
      961 ttagaggaaa tttaatctct ttaataaaaa taaatacaaa agtatttatt gtattagggg
     1021 gatatttttt atggaaaaag aagaatttaa taaattaaaa ataaaggatc aagttgaata
     1081 tataaatcaa aaattagata aaaaaagttt aacggaaata tgtaaagaca taaatatatc
     1141 tagggctaca ataagaaaaa gatttttaaa acaaggatat gaatttaata aaattcagaa
     1201 taaatatata tgttatttag aattaaataa taaggaagag gaagaaaata aatgcaataa
     1261 taatacaaaa gtattagaag aaaaaataca agtcttagaa tcgaaaatag agactataga
     1321 aaataaatta aataattata attcgagaaa tataaaaaag tttgaaggaa aaacagtatc
     1381 aagatgttat agactttatg aagaagttca aaaagaattt agtaaatttt gcaaagaaaa
     1441 ttcaaattat aaagtacaag atatattatc tatggcatta tatgagtata tgaaaaatag
     1501 aaaatgaata aagaaatatt ttctataaat gtttaatggt tagttttctt tatagtatac
     1561 tcatagttaa gttatttaat aaattataaa agtaataaag aattcgagga aaagtttata
     1621 agttaattta cagagagatt aaatgatata agtactatgt aagatagaat aaaaatattt
     1681 aacttttttt tattgtggta aaatagtaaa aaatatttag gaggtaaatt tatgaataaa
     1741 tttgaattat ttaaaaaagt aattgtagga aattttaata atttagagca agtagaaaaa
     1801 gaaaaatctg aaggaaaagt aatacatcca atagcaaaac atgtaaatag aatttgtaac
     1861 gataaaataa aaaacctacc gaaagaattt aaaggtatat ttgtaataga agaaagttat
     1921 tatacagata taaaaactga tagaacaaat atattaccac atttattttt atttgaagaa
     1981 gtggaaaatg gccaagtaaa attaacatca tatgaaattc ctaaagatat atctaaaaaa
     2041 gattttacaa actctaatga agaattaaaa atggattaca atgaattaga agtatcaact
     2101 aaattcatgc ctatgatata tgaatataaa gaagataaag gattttatgg taaaagcctt
     2161 agtgattttg gaagaggaat gacattttta ttagaagaaa cattaagtga aaatgaattt
     2221 gaagtaaatg aaatattaaa aaggggagat caagttttag taggatttga aactccaatt
     2281 atatatagaa gagaaatata aagaaatatt atttattaaa tgttgatata aaagaataaa
     2341 gtaaaatgta gaaaaaatac aaataataca gataaaaata aagtgaaaat ataaaataaa
     2401 ttaattcata tcgaatttac aaaaattaaa aaaataaagt ttaacaaaaa ataaaaaatt
     2461 agaaaaagag tgaatgtaga ggaatacagt tattccaata tgttcactct tttttgtttc
     2521 aactagtccc taaacctaaa ttttaagaat agttattctt catttaaaaa tttaaataaa
     2581 tcttctattg ttactcaaat acttttgcaa tattaatagc tagttttaga gaaggattat
     2641 atctactgtg ctctaaatga atgatagtct ctagctcatt ttgattcata agggattttt
     2701 ctctgtattc ttttatgttt gtttttaaat aaaaaagact agcattttgc tagtctatga
     2761 taaaactatt taacatttta tttataatat ttttgaagtt ttataattag cacttagata
     2821 tcaattttca agctactcag agtctaattc taaatcaaca acattagaat atggatataa
     2881 ctcaggaatt aaattttttg aaaatgcttt aattaaacct acaataacaa tatcactata
     2941 atctttagct gttggaatta tagatataag ctcattttta aataattgta aatttaaatc
     3001 atcaaaatta ttatttctaa taattttgtt aaaagcaaca aaaattttcc ccttatcatc
     3061 aaatgaaatt cctaaatcta aacataataa acttagagaa ataggatttt taatatcatg
     3121 tccgatatgc tcagcgattt cgctaattga atcgtataat aactgctctt tacttgtaaa
     3181 cataaaaagt acctcctaga agaaccaact tgcaccttct tttattgcaa gtgcaatatt
     3241 agacgcaacg ctcctactta ttcctgaatt tactaacaca cgaaaagtag catcatataa
     3301 agcttggtaa ggtatttcag accattttaa taaaggtttt aatcctttac aaattttaga
     3361 ataatgttta gcaaaattct tagcaacagt ttttccagct actttttcta ccacattttt
     3421 agattgatta ggatgcttga taataaattc aatagcctcc ttacaaatct ttgtagctaa
     3481 atttcttgtt tgagacccat ttgagtagtc agattcttta ggtaacggca aaacttcatt
     3541 agatatgtat gcagatttta actgttcgct ctccatagca aaagtattaa taggcataat
     3601 tgctgttatt aatattgccc ctgataaaat tattgataca aatttagatt tttttaaaaa
     3661 tttcatatta atcccctctc ttaaaataat tatcactttt gattattgtt tttacacatt
     3721 tttcaatata tcaaacccct tatttagata atacgtattt atacaaaact tttcaacaat
     3781 tcattacata tttcatttat ttctttttca aaattattta cattaagtct aaccttttgt
     3841 ctaaatatca tagtactatc tgaatctatt tttttgtaat agatcaatag ccctttatct
     3901 tatagctttc atatctatat tttgttcact atttgtattt gtaaatttat cttgttggct
     3961 attatttaaa acttcttcaa attcatcatc actataatta ttatatcttt cactaatatt
     4021 atgaaatcta gttttaactt ttggattttt atcattagtt ttagaaagtg tttttttatt
     4081 tgaattaggt ttataatttt ttctattttc taaaaaaaac atcgaatgtt gttacattat
     4141 ttttaatact attttcaata gtacctacaa catatacaaa agttttattt ttagaatata
     4201 caattatttg aacaaataat tgtatatgaa gtttgtcttt tatggagttt atataaaata
     4261 gtttttaaga aattggagaa tctaattgat ttaagttaga agagagaatg ataatatgaa
     4321 tagatttaat aatagaaaaa ataaagctta aattaagcct tatttttttc tataaaactt
     4381 tcaagccaaa gtttagattt ttcattatta ttaaaataat ttttaaattc atttgctaaa
     4441 tcaatagaat caattcccat actatccatt atagctccta tttcaattaa tctttttgtt
     4501 ctagctttac gttccttttc tttttcttta tttataatag cttgtttttg agcttttaat
     4561 tgtttaatct tttcttctat atctaatagt tttttatttt gttccaaaat aaaattcccc
     4621 cttaatgtta tgtaattaca taacattata atattgaaaa ataaattttt caatattata
     4681 ataaattaac agataaagtt aaaatatagt gtattgatta atcgaaatat aatccgaagg
     4741 gcgcacttat acaccataaa tggtgtgtgc gctcttgcag agggctaaat aaaaatattt
     4801 tatattttta aatacaaaat taatacaaaa gtattaattt tatagaaagg agataatata
     4861 ttttggcaat atatcatcta catacaaaaa taataagtag gtcaaaaggg aaatcatcag
     4921 taggagcaag tgcatataga agttctgaaa aactctataa tgaaagagat ggattggagc
     4981 atgattatac aagaaaaaaa ggtgtagtat ataaagaaat attagctccc caaaatgcac
     5041 ctaaatgggc aacgcataga gaaaaattat ggaatgaagt tgagaaagta gaaaagagta
     5101 aaaatagtca attagcaaga gaaatagatg tagcattgcc tattgaatta tcattagaaa
     5161 agcagataaa tttacttagg gattatgttc aagatatatt tgtaaataaa ggcatggtag
     5221 cagactttgt aattcatgat aaaaaagacg gaaatcctca tgcacatatt atgttaacta
     5281 tgagaccatt tgatgaaagt ggagaatggg gagtaaaggc taaaaaagaa tatatcttag
     5341 ataaacaagg caataaaata tatgataaaa aaggaaatgc aaaatcaaga aaaatagaaa
     5401 ctacaaattg gaataaaaaa gagacactag aacattggag agagcaatgg gcattttata
     5461 caaatagaat tttagaaaaa gaaaatataa aagaaaaaat agatcataga agttacaaag
     5521 atagagagat tgaaaaggtt ccaatgaaac atgaaggtgt agctgtaagg gctatggaaa
     5581 gtagagggat aaatacagag ataggagatt taaatagaga gataaagact ttaaataggc
     5641 agctagagct aatagataag caaataaatg ctcaagaagg catcaaggag gttttatatg
     5701 gatttaaaga taatggagct agaaacgaag ataacagagt tagaattaga gataatgaaa
     5761 tggaacaagg atatagagga tattcagttg ggactaacgt tacagacgga tcagcagttg
     5821 aaaaacttaa gcaactcgct agagagaaaa aagaacgaga tagagaatat cgagaaggaa
     5881 ttgaaagaga aaatagaaaa cgacttgagc aagagcgaca aaaatcaaat attaaaacag
     5941 ttagaagaag tagaaatagc gagatggaat tataatcgag aaattaaaga gataaaatca
     6001 gatataagag acttaaaaga gtgtaacaac gatataaata ggaaatatga agaaattaag
     6061 gaaagtatcg gtttaattaa aaataaagat aaagaaatga aaaagaaaag aagagtagta
     6121 atagggataa ttatatggtt agttatatca ctgtatatga atcttttaga cgatatagaa
     6181 ggaattaaga ataaaataat tagagaacat ggatatggaa tgacaattca agataataaa
     6241 gaaattatgg catttattca gaaaaaggga aataaataca aaatatgata taatattgat
     6301 attatctaca agtaaggggg tgagataatg aggaatacat taataagagt agatgatatt
     6361 gatgagagta ttgaatatat agatacaatt catggagaga gtaaagggtg gattacaaag
     6421 gctgaaataa ataaaaataa agaatttagc caatggcatt atcttgtagg agatttgctg
     6481 aaacaagatt ttgataaaga gaatatttat atttctatga gtacgtttta taagccaatg
     6541 agacggattg aaacgataaa agaaatagga agtctattca tagaccttga tacatataat
     6601 actcaattca ctaagactca aatacttatg aatttggaag aaaattattt taatagatca
     6661 atcccaatac caaatttaat aattgatagt ggaagagggt taactttagt ttggagtatt
     6721 gagaaagtac cacataaggc tctgccgtta tggaaagcag ttcaagagta tttatatagt
     6781 caattaaaag aatttggagc agatagaaaa gcactagatg taacaagaat tctaagagta
     6841 ccaggaagta taaattcaaa gagtggaaca agagttacta tactagaaaa atatgaatat
     6901 aagtacactt tgagagaaat acaaagtgaa tttctacctg atttagatga aaatagacca
     6961 aagaaaaagg gaagacctaa aaaagtagta tatattcata gggaacgttc attgtatcaa
     7021 ggaagaatat tagatttagt aaagttatgt gagttaagaa actatgatgt aaaaggtcaa
     7081 agagaaataa tattattctt gtatagatat tatttatgtt acttctatga agatgaacaa
     7141 aaagccttag aagacgtttt agagcttaat aaggagttta tacaaccatt aagtgagaaa
     7201 gagcttataa tggcaacaaa tagtgcagag aaggtattta aatcaaagga caagcagtat
     7261 aaatacaaaa atgaaacgct tatagaactt ttagaaatat cagagtatga acaaattcat
     7321 atgaagatta ttattggtaa agaagaatat aagcgaagaa ataatgagta taataagaaa
     7381 aagtataagg aaaaacttaa acaattaggt aaaatgacaa aacaagaaga gttaaatatt
     7441 ttaagggaaa aaataaaagt ccttagagag aaaggactta aaaatagtga tatagcaagt
     7501 aaattaaaca ttcctttaaa aacattaaag cgtcatatta cttatatgaa aaagaatgac
     7561 ttattggcgt aagtcattct tttaatatta tgttttataa atgaatttta acatgatatt
     7621 gaat
//
LOCUS       DAKBNN010000185         7604 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000185 DAKBNN010000000
VERSION     DAKBNN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.186"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            198..1517
                     /locus_tag="OUB02_003165"
     CDS             198..1517
                     /locus_tag="OUB02_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCT8886896.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(1592..2020)
                     /locus_tag="OUB02_003166"
     CDS             complement(1592..2020)
                     /locus_tag="OUB02_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886897.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTSTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(2111..3487)
                     /gene="hydG"
                     /locus_tag="OUB02_003167"
     CDS             complement(2111..3487)
                     /gene="hydG"
                     /locus_tag="OUB02_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HCT8886898.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(3488..4573)
                     /gene="hydE"
                     /locus_tag="OUB02_003168"
     CDS             complement(3488..4573)
                     /gene="hydE"
                     /locus_tag="OUB02_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HCT8886899.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(4609..5817)
                     /gene="hydF"
                     /locus_tag="OUB02_003169"
     CDS             complement(4609..5817)
                     /gene="hydF"
                     /locus_tag="OUB02_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HCT8886900.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(5832..6083)
                     /locus_tag="OUB02_003170"
     CDS             complement(5832..6083)
                     /locus_tag="OUB02_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HCT8886901.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(6464..7504)
                     /locus_tag="OUB02_003171"
     CDS             complement(6464..7504)
                     /locus_tag="OUB02_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCT8886902.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         2575 a         1173 c         1060 g         2796 t
ORIGIN      
        1 aaaaataatg tttatttgat aaaaataaaa tatattgttt ctgaataaca aatattttga
       61 aaattataaa aacatatatt gacaatttgt gtcaaaaata ctaacattaa gtatataaag
      121 aattttatat gagaatatct ttataaatcg caataatttt aaaacataaa tttatttaaa
      181 gaggggatga tttgccaatg agcgagataa ctagtcaaat gataagtaca gaagaaaaaa
      241 aatatgttgc taaaactcaa aagatacctt attatccagt agcatttaaa tcaggtgatg
      301 gtgcaatgct atatgattat gaaggtaatg aatatgtaga ttttttagca agtgcaggct
      361 ctgcaaatgt aggtcatggt aataaggaaa tctcacaagc agttaaagaa caaatggatg
      421 atataacaca atatacactt gcatatttcc atagtgatcc tcctgtaaaa ttagctgaga
      481 agcttgtgga aatagcccct ggagataatg ataaaaaagt actatacagt gcaactggtt
      541 ctgcttgtat agatgctgct ataaaattag caagagggta tactggaaga acaaagataa
      601 tatctatgtg tgaatcttat catggaagta cttatggagc tatatctata tctgctttaa
      661 gtacaaatat gagaagaaaa atgggtccat tgttaccaga agtttatcac ttccattatc
      721 ctgataaaaa tagaacagct aaagaatgtt tagatgagat agaatatgct tttgcacact
      781 atctaccagc agaagaggta gctgcaatat ttatagagcc aattgcagga gatgcaggaa
      841 taatagtgcc tccagtagag tgggtacaag gtttaagtaa aatatgtaaa gaaaatggaa
      901 tattacttgt aagtgatgaa atacaacaag gtatgggaag aacaggtaag tggtttggta
      961 tagaaaattt tggtgttgaa gcagacctta tagttcttgg aaaatctgtt ggaggtggat
     1021 tacctctagg agcagttgtt ggaagaacag aaataatgca aagtcttgat gcaccagcac
     1081 atttatttac tttagctgga aacacaacag tatgtgtggc tgctttaaaa tctatagaga
     1141 taattgaaaa agaaaactta cttcaaaaga gtatagaaat gggagattat attaaagcag
     1201 gatttgaaaa actaaaagaa aaatacgata taattggaga aataagaggc ataggattat
     1261 ctataggagt tgatatagtt aaaggaaaag gttctaatga aaaacatcca gatgctacag
     1321 ctaagatatg ttatagatgt atacaaactg gattaataat gatattctta ggacaatcta
     1381 ctttaagagt gcaaccacct ctagtaataa ctaaggagca agtagataaa gcaatgaata
     1441 tcatagattc tgcaatagat gattatttaa atggtagaat tggtgatgaa gtatatgaag
     1501 taactcaagg ttggtaaaat aaatattaaa tgaaaatagg agttatctca attttagaaa
     1561 attatttctg aaaagagata actcttttaa gttacccaca aataagaaaa ttgtttgatt
     1621 cattaaagac tatatttcgg atgtcgtttt tgtgagtatc agagacagta ttacgagaat
     1681 aattaccttt attcgtacta tctgttaatt tagttataat taagatagct aaaagaataa
     1741 gaattactgt gtataaagca ataacatatg gttttctttt atatttgtat ttgttattga
     1801 aaaaagaatg gccataatta tttttattta ctttagatgt acttgtattc tttttcttgt
     1861 tacctttctt tttatcatcc ttttttctat ttttagggta aggaatgact ttgtgtgtat
     1921 tatcaagttt gatattttta tttttttgaa tattgttttc caggctagcc tttttttctc
     1981 caaattgaat agttttattg gtattgttgt tatctttcaa tgttaatctc cctcccttaa
     2041 actttccctt tagataatat tactactaac ctatggaata tacaataaat aatatataag
     2101 taataaaatt ttataggtat aaatctcttt ctccacgctc taaggcagtc atattgttag
     2161 atactatgtt tcttatatct tctctttcta tcttacctat ttcttctgcg ataagttttt
     2221 tacccataat tttaagttct tcatctccat aatcgattaa aaattcattt aatgtagtaa
     2281 gagcatttgg tgtacataca ttatggattt gtcgactttt agctaagctc atgaatctat
     2341 cgccagttct acctttacga taacaagctg tacaataact aggtatatat ccatcagtaa
     2401 tcagttcttt aagcacctca acagggcttc tatggtctcc aacttcaaat tgttctgtat
     2461 tgtcaccatc ttcatatgct ttatatcctc caacacctgt tagagaacct gcactaattt
     2521 gtgatacacc atattttagt aactcatttc tcatttcagc agtttctcta gtggacatta
     2581 tgattccagt aaaaggaaca gccaatctag ttatagctac tatttttttg aacatatcgt
     2641 cagaaactaa gtgaggaaaa tcttctaagc tcataccttc agcttttttc aatctaggga
     2701 aagatatagt atggaaacct acaccaaatt tttcctctaa gtgttcgttg tgcatcataa
     2761 gtcctagaac ttcaaattta gggtcagata aaccaaataa aactcctgca ccaacatcat
     2821 caacaccagc ttccatagct ctatcaaaag ctgttaaatg gtaataatag tcatttttta
     2881 tagattgacc atgcattttt ataaatgttg gtttgtgata agtttcttgg aataatatat
     2941 aagttcctat tcctttttct tttagtaatt tatactcatc tacacttgta gctgctatgt
     3001 taacgtttac ccttcttata tttccgtttt cattatatgt tgaataaatt gcatctaagc
     3061 aatctaagat gtaatttata tcacaatttt tagggtcttc accagcttct agagcaagtc
     3121 ttttatgacc cattttttct aatattttaa cctcttcctt tatctcttcc atggtaagtt
     3181 tttttctttt aaatttgttg catttactaa aaccgcaata aacacattca tttacacagt
     3241 agtttgatac ataaagaggg gcaaataaaa ccactctatt accatatatt tcatttttta
     3301 tttgcccagc tatactaaat aatctatcta gttgtttttt atcttctact tcaagaagtg
     3361 tagctatatc tttataacta agtttttccc ttctatcagc cttatcaagt accttttcaa
     3421 tatcatcaga agttgaattt tttgcatctt ctaataaaga attaataagt tcatggttta
     3481 tgaacattta caaacgcctc cattcaatat tatcaccgcg agaatagtca acttcaagtc
     3541 ctaatgactt gatgttttct tctaaacttt ggtgatattc acaagcttct tgaccagtaa
     3601 aggctttgtt atcgtaaaga gaatattgtt ttctaaattc ttgaggagaa aggtttggca
     3661 taattacatt gcaaccagct aaaagacctg catttctacc actagggttt atagatgcca
     3721 aggctgttgt agcgggtaat aaaacttttg gaagtagaag tcttgtaatt gaaagcataa
     3781 gtaatgtctt ctctaaatct ccatgtttat aatccttaaa aattgtatca tggtgtggaa
     3841 taaaaggtcc tattccaacc atatgaggat ttaactcttt taggtataat aaatcacgaa
     3901 caaggtcttc gttgttctga aatggtgatt caaccataaa tccagcacct acctgataac
     3961 caatttcttt aaggtttttt aggcattctt ttctagttct aagctcaatg tttggcggat
     4021 gtaatttttt ataatgactg tctgtagcag tttcatgtct taaaagatat ctatctgcac
     4081 cacataaaaa gtatttttta tacgaatcat aactcttttc tcctagtgat agagttatgg
     4141 cacaatcctc atatttattt tttatacttg aaacaatttc acatatttta tcatcagtaa
     4201 aatatgcatc ttcaccacct tgcaaaacga atgttctgta tcctatttta taacctatat
     4261 cacaacattc aagtatttca tcaagtgaaa gcctgtatct tatgatattt ttattagaag
     4321 attgtattcc acaatagtag caattatttt tacaatagtt tgtaagctct ataagtcctc
     4381 tcaagtagac tctatttcca taatgtttat ttctcatgtc gctagcttgt ttataaagat
     4441 agtttcttaa attttgttcg aagttatatt cctcattgtc gatattttct attaaatata
     4501 gtagttcatc aaaacttaat gaattaatgc tgtaaagttt atctataaag tatttaatct
     4561 tatttttagt catattcaaa tatgtaatta ttaagtatag ttaaattttt aataattgaa
     4621 aagttcaagt gatctttcca aaataccttt tacatatgct attaagacac catagtttac
     4681 aataggtaca ttgaaagatt ttgccttttc tattctagat aacattccag ctctattcat
     4741 catacaagct ccacagtgaa ctacaagggc atatttattt atatcttcag tgaaggatac
     4801 tccagaacta aattcaaaag taatgttttt gccagttttc tttctaatca tattaggaat
     4861 ttttactgtt ccaatatcat cagtttgtct atgatgggta catccttcgg ccatcaagat
     4921 tttatcacca tcttttaaat tttctaaagc ataagctcca ttaatcaatt ctttaaggtc
     4981 tcccttttgt ctagcaaaca atatagaaaa agatgtaaga ggaatatctt ttggtgtatc
     5041 tttatctact tgtggaaata cttgagagtc agtaataaca agttttggtt ttttgcctaa
     5101 attagataga gtttctttta aactatcttc ttttgtaact atagaaattg caccattatc
     5161 caaaatatct cttataactt gctgttgagg aagtataagt ctaccttttg gagctgcttt
     5221 atctattgga acaactaaaa caactaaatc atttggttca attaaatctg ataccaattt
     5281 gaactcagtg ctatccttag gcaaaacttt tattatttca ttttttaaat tttcaattcc
     5341 aattttatct gttgatgaga cagaaacaac tggacattta actttatttt tagttaagtt
     5401 taaaatttct gattgattct taatagtatc aattttgtta agaattagaa tatgagggat
     5461 attcttatca ttaaattttt caattagtga caaatcttct tgtgatatac caatttggca
     5521 atccactact agtaaagcta tatctgtctt ttctaaaaca tctagtgatt tacctattct
     5581 aagttcacca agttcaccaa catcatcaag accagcagta tctataagaa cacaaggtcc
     5641 tataggaagg atttccattg gtctaaatac tgggtctgta gtagttccag caatatctga
     5701 gactattgca gcactttgat ttgttatagc atttattatg ctagatttac ctgcatttct
     5761 tttaccaaag agacctatat gtactcttac cgattgtgga gtagaattta gactcatttg
     5821 tactcaccac cttaaacttc tttttttgat atactagttt ttacagatac atgaggtatg
     5881 ttgcccaatt taccagtcaa actattaata gtatccattt cgccaacaac tgctatacat
     5941 acgattgaga caccttcttc ttcgcaagga atgcccattc ttcctttaat aataccttta
     6001 aaacctgata caacctcatt gaatttaaat tgacattctt ttggctcttc caaaatagca
     6061 cttataactg caaccttttt cataaatatc ctcctaactc agaaaatatg tcttaaacaa
     6121 atccaaaatc aaaaaacttt tctttgtaaa gaaaagtttc tacaaaagaa aagttattaa
     6181 ttacacaagt ataatgtgct atattaaact atttccttac agattagata actcttttct
     6241 ggtagggtta gcttgtaaaa atttaattta acaacaagta actgaagaaa atttttatac
     6301 aatttatata aattataata aaaattaact aaagaaaatt gaagttactt atataatagt
     6361 agtaaattga aattaagtct atagaaaaac caatttttgt ataaaaataa aaattaaagt
     6421 taattatttg ttaagaattg actttggtaa aatatataat taattattct ttaagcatat
     6481 aatttataag taattttagt gtattttcaa tagcttctat atgtgttctt tcataatggt
     6541 gactattgtt tgttccagga ccaacacatg ctatttgaac atcttctccc caacgaatag
     6601 cttctgaagc atctgaacca tagaaagtaa atacatcgac agcgtagtct aaattattgt
     6661 cttctgcaac atcgacaagc ttgtttctta aattaaaatc atataatgat aaattatctt
     6721 ttgcaactat agttacacca tgttcactag atgtttgact tggtccagta ggtgctatat
     6781 ctattgctac aaattctttt gttttttcag gtaaacattt agaaacacca tgtccaattt
     6841 cttcataatt acttatataa aagttagtag tatgcttagg tgttaaattg ttttctttaa
     6901 aatatctaca tatttcaaga accattgcta cagctgattt attatctata tatctagatt
     6961 ttataaaacc agattctgta gcctcaaatc tatggtctag gcatacaaaa tccccaacac
     7021 ttattcctaa atctaataca tcttgctttg taaatacttt ttcatcaagt ctaataatca
     7081 ttgtttcttc atttctttca gtggcagctt ttgtttgacc atatatatga gtagaagcat
     7141 acttaaatac aacacttcct ctgatttttt taccttttct agttataaca gtacagtttt
     7201 ctccctctat agctgcccaa catccaccac caactttatg gtatttaaga gtaccatcag
     7261 atgatatttc ttttactata gctccaagtg tatccatatg tgcagatatt gttatttgat
     7321 ttgaatcatc ttcaccagga agagtagcta taagtgcacc tttttttgta agaatagtgt
     7381 caagacctaa attttcaaaa tcttttttga tttctaaaac tgcattttca gtatatccag
     7441 ctgggcttgg aatgtccaaa tattttttca ttaaatttat agtattagtt aaatttaaat
     7501 tcatattgtt tcctcctaaa taaatatatt tatacaaatt ataacataaa tacaaatgtt
     7561 ctgaaaacta aaattaaaag aatagaatct aaacatattt aaaa
//
LOCUS       DAKBNN010000186         7591 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000186 DAKBNN010000000
VERSION     DAKBNN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.187"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            3..314
                     /locus_tag="OUB02_003172"
     CDS             3..314
                     /locus_tag="OUB02_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCT8886903.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKKIEQL
                     T"
     regulatory      407..499
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            599..1393
                     /gene="thiD"
                     /locus_tag="OUB02_003173"
     CDS             599..1393
                     /gene="thiD"
                     /locus_tag="OUB02_003173"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCT8886904.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1417..2205
                     /gene="thiM"
                     /locus_tag="OUB02_003174"
     CDS             1417..2205
                     /gene="thiM"
                     /locus_tag="OUB02_003174"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCT8886905.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2228..2860
                     /gene="thiE"
                     /locus_tag="OUB02_003175"
     CDS             2228..2860
                     /gene="thiE"
                     /locus_tag="OUB02_003175"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCT8886906.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            2999..3301
                     /locus_tag="OUB02_003176"
     CDS             2999..3301
                     /locus_tag="OUB02_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCT8886907.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3367..4116)
                     /locus_tag="OUB02_003177"
     CDS             complement(3367..4116)
                     /locus_tag="OUB02_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886908.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPFILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4110..5072)
                     /locus_tag="OUB02_003178"
     CDS             complement(4110..5072)
                     /locus_tag="OUB02_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886909.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNSSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5076..5534)
                     /locus_tag="OUB02_003179"
     CDS             complement(5076..5534)
                     /locus_tag="OUB02_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCT8886910.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5889..6245
                     /locus_tag="OUB02_003180"
     CDS             5889..6245
                     /locus_tag="OUB02_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8886911.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6317..7033
                     /locus_tag="OUB02_003181"
     CDS             6317..7033
                     /locus_tag="OUB02_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886912.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7026..>7591
                     /locus_tag="OUB02_003182"
     CDS             7026..>7591
                     /locus_tag="OUB02_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886913.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGIISNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIA"
BASE COUNT         2964 a          888 c         1244 g         2495 t
ORIGIN      
        1 aggtgtattt tacatatatt ataagatgta aagatgattc tctatacact ggatatacca
       61 gtaatatagt tagaagaatg aatgaacata agttaggaat taattcaaaa tacacaagag
      121 caaagggatt tgaaaaacta gaagtttatt ttgtgaccaa cacaaaaagc aatgctatga
      181 agttagaata ctatataaaa aaattaacta gaaataaaaa attgtctata ataaaaaacc
      241 caagtatact tataaattta attgataata aagaagatta tattattggt aagaaaatag
      301 aacaattaac ataaatattt atagtaacat tataatgggt gcatatggtg taaaaacttg
      361 acaataaata ctattgtaat ataatgaagt tataaatata gtaaattaaa atagctaggg
      421 gagccaagag ctgagaggta taccgaccct tacacctgat ctggataata ccagcgtagg
      481 aaagcttagt aaaaattcaa gtatgtatta tttgtgatat atgctatttc taagctatac
      541 ctattgtagg tatagctttt ttaattaagt tatttaaatt aaactttagg aggcaacaat
      601 gagtaattat aaaataccaa cacttacaat agccggctct gattcatctg gaggagctgg
      661 gattcaggct gatttgaaaa cgtttagtgc aattggtact tatggtatga gtgtaataac
      721 agctataaca gcacagaata ctaaaggagt ttttgctgta gaggatttaa ataagaaaat
      781 aataaaaaaa caaatagagg cagtctttga agatatacct ccaagagctg taaaaatagg
      841 aatggtttca agtcctgaga ttatacttga aatagttgag aatctaaaga aatacaatcc
      901 aaaatatttg gttgtggacc cagtaatgat atctaaaagt ggatattatc tattaaagcc
      961 agaggcaaaa gaaaacttaa ttaagtattt gataccactt gcatacataa ttacaccaaa
     1021 tataccagag gcagaagaaa taacaggaat aaaaatacat aatgtagatg atatgaagag
     1081 agtaggagaa gaaatactac aacttggacc taaatttgta cttatgaaag gaggtcattt
     1141 agatggagaa gcagtggata ttttagttgg taaaaatata tttaaagttt ataaaagtga
     1201 aagaattgat aaaaaaaata cacatggaac agggtgcaca ttatcttctg caataacatc
     1261 ttatctagca ttaggatatg aaataacaga agcagtaaat ctaagtaaaa tctatataac
     1321 agaagcaata aaaagaagtt ttgatatagg tcatggagta ggaccagtac atcattttta
     1381 taaatttgag taataaaaaa atattggagg taaaatatgt ataatttaat taaagatgta
     1441 aaaaaattaa atccattggt aattcattat actaataatg taactataaa tgattgtgct
     1501 aatgtaactt tagctgtagg agcaagtcct cttatgagtt tttcatatga ggaagtagaa
     1561 gaaatggtaa gtgtagcaaa ctctgtagta ataaatatag gaactatgaa ctcaaatatg
     1621 ttggatttat tcctattagc tggaaaagca gcaaataagt acaataaacc tgttgttttg
     1681 gacccagttg gagtatttgc aagcaaagcg agagcagaac ttacaagtag acttttaaat
     1741 gaagtcaaat ttagtgttgt taaagggaat gtatcagaaa taaagtttat aggtggcttt
     1801 aatgtaaagg gtaaaggtgt agattcattt gatgaagaag aagattctac tgagataata
     1861 agaaagattg ctgaaaagct tgaatgtgta gttgtagcaa ctggaaaaat agatataata
     1921 actaatggta aaggtacata taaaataaac aatggaactg ataaattaaa aggaattaca
     1981 ggaactggat gtatgactgc tagcttgatt gcaagtttta tggctgttac tgagaatata
     2041 ttagaagcag caacaatggg tgttcttaca atgtctttaa gtggggaatt agctaattta
     2101 aataatccac caataggtac atttaaagaa aatcttatga atgctattta tcaaatggat
     2161 atagatactt taagtaaaaa ttctaatatt gaatttttaa attaataaag agtaagggag
     2221 acttaaaatg attgataaag aatctttaaa aaaatgttta aagttgtatt tagtaactga
     2281 ctctgaaatg cttaaaggta gagactttta taaatgtctt gaagatgcaa tatcttctgg
     2341 aataacaact gtacaactta gagaaaaaaa tgcttctgga agagagtttt taagaaaagc
     2401 tatgaaactt agagaaataa ctaaaagata tggtgttaag tttataataa atgatagagt
     2461 ggatatagca cttatttgtg atgcagatgg agtacatgta ggacagagtg acattgatgt
     2521 tagagaggtt agaaagctta taggaaacaa taaaattcta ggagtatcag caagaacttt
     2581 agaagaagct atttgtgcaa aaaatgatgg cgcagactat ttaggtgtag ggtctatatt
     2641 cacaacatct actaaattag atgcaaaatc agcttcgttt gaaactgtta aagaaattaa
     2701 agaaaaagta gacatgccat ttgtactaat tggaggtata aatttagaca atatagataa
     2761 acttaaatgt ttagaaagtg acgggtatgc tataatatca gctatattaa aagctgaaga
     2821 tatctctaaa gaagtggaaa agtggacatt aaaaatataa aattggctct ggtagtgaaa
     2881 aaaatggaaa aatttgatat aatctaagag taaaatttaa gcaattataa atgataaact
     2941 tttctacata tactttttaa tcaagatata tgtagatttg gttaaaaagg agctataaat
     3001 gattaggata ttgttagtgt gtgttggagg catgtcttct accctattgg taaataaaat
     3061 ggagaaggat gcaaaaaaaa gaaatataga ctgtaaaata tgggcagttg gagaaggaga
     3121 tataaaatcg gagcttgata actttgatat tttacttctt ggaccacaac ttagatttat
     3181 gcttgatgac gtaaagtcta tagttggaga tagagctcca gtatctataa tagatatggt
     3241 aaactatggc acttgtaatg gtcatgctgt gttaaattcg gttttagaaa tattaaaata
     3301 aataataaaa gtggattcta tataatagag tccattttta ttatttgtta ttaataggct
     3361 taaaacttag cttaccattt ctttttttaa tctccacata gcaagtgaaa aacttgaaca
     3421 gcacaatatt aaaaatagta ggataaaagg cagtatatct ccaataagaa tagtctcacc
     3481 aaatattaag ctacgtaaag cgttaataac atgggtaaat ggattgagca tcactgctac
     3541 ttttaatcct ccagataaac tttctatagg gaaaagagct gaacttaaaa agaaaatagg
     3601 aagtacaatt gcagtcataa ttgtttcata aactatttca tttggcaata ataaacttat
     3661 agaataagct agacttgata aaaagaaggc tgtcataaat atcagtacta tcataagtag
     3721 gattccacca atgccagatt ctatccttac tgagaagaat atacttacta tacacaaaat
     3781 agttacttct atgaaagaaa ctaagatagc ttctaataat tgaccaagga cgattgaata
     3841 cctgctgata ggtgcaatta agactctata gaaacttcca ctgttcttca ttataaagtt
     3901 tataatacca ccactactac aagaagaaaa cacaaccagt acaatgatac ctggtagtat
     3961 aaaagcagta taattattga tatttatatt attcatagtt tggtttgcaa tagaactata
     4021 aagcacaagc caaagaaaag gttgtaatat agtaactaca aatgagattg gatttttaaa
     4081 acgccacttc atactacgcc ataagatatt taacattccc attcctcctt taagcttttt
     4141 ttagaacttg taagtgctaa aaatacatct tcgagacttg gctctacaat ttctattgca
     4201 tcaaattcta tatcatgttc taaaagccat ttatttatta aagtaaaagc agttctacta
     4261 tcgtttacac ttataaaaat tgaattttct cgtacgctca taaactttac taaaccagta
     4321 cttttaattg aatctttata tttttttata tcttcagtat tatgaaaagt tatacgtagt
     4381 atattttgtc taatgtaact acgtaaactt gatggtgttc cttgtgctaa atcctttcca
     4441 ttcttcataa tacaaatatt atcactcaat tgttcagctt cttcaagata atgagtagtt
     4501 agaaatatag ttgtgccata ctcatctcta atttttaata acatatccca catagatttt
     4561 cttgaatcga catccatacc aacagttggt tcatctaaaa ataggatttt aggagatgat
     4621 accatattca tagctatgtc caatctacgt tttacacccc ctgaatatga tgaagtagga
     4681 tattttaagt aactagataa gtcaaactta tcaattaaag agtctattct ttgttttgca
     4741 atctgtggtt ctactttata cattttactt tgaaatacca tattttccat aagggataaa
     4801 tgttcatcaa tagagatttg ctgtgctaca caagcaattt gagtacgtat ccaagaagaa
     4861 ttatctacta aatcttttcc aaacattttt acatttccag atgtaggttt gtaaaaggta
     4921 gtcaaaatat ttataagaga agatttacca gcaccattag gaccaagtaa agaaaaaatt
     4981 tctccagcat ttacattaaa gcttagacca tttaaagctt tcacaccatt tttatattct
     5041 tttactaaat tgtctatttc aatcgctaac atatattatt cctcctcttt aagtggaaat
     5101 attatctctg taatatagtt atctgggttt ccttttatca acatacctgg tcctttaata
     5161 aatacttctc tccaaggagt ttgtattttt atattatttt catgaatata tttaaatatc
     5221 atttcatacg tttttggcaa attactataa gagcctgtgt gagtaaaaaa taaagctttt
     5281 gttttaggaa attcttttgt agttataccc atacgatttg gagtttcagc agttggaaca
     5341 cataattcca tttctgcaac accagttttt tggtcaacat catagtagca aaagaatggt
     5401 gcaccgttag actttccttt tatagcttta aatacatttg gaaaatactt tgttgcctca
     5461 gtcataggtc cattataacg catggtagcc actgtgactg attcgatatt tttgatttca
     5521 attttgcaat tcatttgttt acctcctaat gtgtgttcat attcttacta gatttttaag
     5581 cattaataca tataagattt tgattagttg aaaacatata atcttatctc gtttgcttgt
     5641 tttcaagtct aacataacat aaaatatata tcctctcatt tgatgctcca ttttgtaaag
     5701 ttattattta agctttactt tagattttta gcaaattaaa ataaagcaat ttataaaata
     5761 agtatttaca aattaagtgt attaatcgta taatacactt aataggatac tagataaagt
     5821 ctgaaataaa ggcgtatttt tttaaagtaa gtgtactatc gtattaatac actagaatag
     5881 gaggaatcat gaattttgaa ttagataata caactcccat ttatttgcaa atagtaaagt
     5941 atataaagag acaaatagta acaggagagt tgaaaccagg agagacaata ccatcaagaa
     6001 gagaaatggc acttaatcta aaagtaaact taaatacagt tcaaagagca tataaggaga
     6061 tgggtgatat gaatataata aatactttta aaaattatca aagcagtgta acagttgatg
     6121 aaaatatatt aaaaaattta aaattagagc ttataaatga atcactatct gtttttatag
     6181 aagatatgaa agcaataaat gttagcaaag aagaagtttt aaaaataata gaagataagt
     6241 attagttttt taacaaatag aaaacttagt ttagaaagtt tatttataag catattggat
     6301 gtggaggata gtaaatatga tagaaataaa gaatgtcagc aaaacatata aaaggatgca
     6361 agggcttaaa ataagaaaga tagaagcttt aaaaaatgta agttttaata tagaaaaggg
     6421 aaaaataact gcattacttg gtataaatgg tgttggtaaa tcaaccatgt taaaagcaat
     6481 agcaggactt ataaacattg atagtggaga aattcgtata gatggagaaa aaataaatga
     6541 aaaagtatac aataaattgg cttttgttcc agatgtacaa tctcacttta gcaatacaac
     6601 aataaaagaa acatttgaat ttatggaaat tttttattca aagtggaata aagaaaaatc
     6661 aaaagaaatg atggacatat ttaaattgga tgaagatgaa ataattgata acttgtcaaa
     6721 aggaaacatt gcaagagtaa aattaatact tggattttgc caagacccag agtacatact
     6781 tcttgatgaa ccatttacag gaattgattt gtttaagagg gaagagttta taggagtaat
     6841 agcgcaatat atggaggaaa atcaagccat tatcataact actcatgaga tagtagagat
     6901 agaaagttta gttgatgagg ttgtaattct tgatgaaggg cagattataa cttcatttaa
     6961 tgcagaagaa ttaagagaaa gagaaggtaa atctatatta gataagatga gggaggtata
     7021 taagaatgaa taaacttcta tatctaataa atgtagattt gagaagaagc aataagtttt
     7081 atatagctta tatatcattt ttttcattaa ttacacttag tttaaattta ttcgaaatta
     7141 ataaatttaa aaattctaaa tttattatga acttagcaat agaagatttt ggaggtatat
     7201 tttatggact agggataatt agtaatcttg gttttatcca aagtatattg ttctttggga
     7261 tattgggact ttttatatac tatattttta tgtggaaaag agagttcttt tcaaataatc
     7321 aaagtattta tacacttatg atgttacctc aaaataagtt taagatttgt atatctaaat
     7381 caatagcctt gataacaatg atatatggtt ttttgataag tcagacagca acattattta
     7441 tatgtaaata tatatttaat ttcattttta gaaatatgcc tataatcaat atgaactttg
     7501 ccaaagattt atattattta aacttgaaga gtataccaat tgattttatc tcttttatgg
     7561 ctatttatgt atttttacta cttatagcta t
//
LOCUS       DAKBNN010000187         7540 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000187 DAKBNN010000000
VERSION     DAKBNN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.189"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            124..633
                     /locus_tag="OUB02_003183"
     CDS             124..633
                     /locus_tag="OUB02_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCT8886914.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            645..1526
                     /locus_tag="OUB02_003184"
     CDS             645..1526
                     /locus_tag="OUB02_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HCT8886915.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1654..1859
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1985..3256
                     /gene="serS"
                     /locus_tag="OUB02_003185"
     CDS             1985..3256
                     /gene="serS"
                     /locus_tag="OUB02_003185"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCT8886916.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3577..4032
                     /locus_tag="OUB02_003186"
     CDS             3577..4032
                     /locus_tag="OUB02_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCT8886917.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4033..4124
                     /locus_tag="OUB02_003187"
     tRNA            4033..4124
                     /locus_tag="OUB02_003187"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4067..4069,aa:Ser,seq:gga)
     gene            4162..4426
                     /gene="ffs"
                     /locus_tag="OUB02_003188"
     ncRNA           4162..4426
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OUB02_003188"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4503..6140
                     /gene="dnaX"
                     /locus_tag="OUB02_003189"
     CDS             4503..6140
                     /gene="dnaX"
                     /locus_tag="OUB02_003189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCT8886918.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6199..6549
                     /locus_tag="OUB02_003190"
     CDS             6199..6549
                     /locus_tag="OUB02_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCT8886919.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6562..7161
                     /gene="recR"
                     /locus_tag="OUB02_003191"
     CDS             6562..7161
                     /gene="recR"
                     /locus_tag="OUB02_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCT8886920.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7302..7523
                     /locus_tag="OUB02_003192"
     CDS             7302..7523
                     /locus_tag="OUB02_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886921.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         3070 a          756 c         1392 g         2322 t
ORIGIN      
        1 tcttttttat ttattataaa agttaagatt aaatagaata taaagtcaat attgatttat
       61 aataagataa aatataaaat tatatagtat aggtaaagtt tttatttgga aagaaggtgt
      121 aatatgaatt ctaatgaaag aagaagtaaa ttaatagata ttttaaaaga atctaagcat
      181 cctgtaaaag gtggaacact agctgaacta ttaaatgtaa gtaggcaagt aatagttcaa
      241 gatatagccc ttataagagc tagaggattt gaaataatag ctacacctca aggatatata
      301 atatataatc aaccgtattt tacaaaacaa ataaagtgta aaaatcataa ggattctaaa
      361 gaaatttatg atgaattaaa gataattgta gatttaggtg gtattataaa agatgtcatt
      421 gtaaatcatc caacatatgg tcagataact gcagaactta atatttattc taaaatggat
      481 atagacaact ttatgaagaa agttgagaca aatgaattta aacaattatc ggttctgaca
      541 gaatgtagcc atattcatac aattgaggca ataaaagaag aaactataga agctatagta
      601 aaagagttaa aggacaacgg aatattatca taaggaggag ttgaatgaat tttttatcat
      661 tggctaagac tgcctcaaca aatagcaata taactaagag aactttagaa aatatgctcg
      721 ataattttag tgataatata ccaaatatta tatatgcaat aattgtattt gtgataggta
      781 tttatatatc taaaatagta agaaggatgg tttcaaagtt tctaatcaaa tatggaatga
      841 gtaaaggtgt aaataatttt attgtatatg gaatatatat atctatgtta tctattatat
      901 cattgataag cttaggtatt atagggatac aaactacatc agttgtagct gtgttaggag
      961 ctgctggatt tagtataggt ttagctttta aagaaatatt atcaaattta ggctctggta
     1021 tgattatttt attttttaag ccatttaata taggtgatta tattcaaggt tcaggtgtag
     1081 aaggtacagt ttctgacata caaatattta gcactgttct taagacacca gataataaaa
     1141 ctataattat acctaatttt caactgacaa gtaataatat aattaattac acacatcaaa
     1201 acaaaagaag aatagatttt tcatataata tttcatatga tagtgatata gatgtagtta
     1261 agtctatatt aaatgaaatt tttacaaacg aaaaaagaat attaaatgat ccaaagccta
     1321 taataggttt aaactctata ggaaataaca ccatgcaaat agttgctaga ccttgggtaa
     1381 aaactgatga ttactgggat gtatactttg atgtaatgga aaaggtaaaa aataaatttg
     1441 atgagaatga tataaaagtt ccatttgttc cttcttcatt attatttaat aatacaggta
     1501 atctaaatac aatggataaa agataaggtc ttaattaaat agaaaaaatg aatttaatat
     1561 aaaaaattaa agaaaattct aaaaaaattt gaataatagt attgaaagat gttaatatat
     1621 ataatataat gattatcaaa taaaacaatt agttaaaaag ctgtgaagga aaatagtaaa
     1681 aaacagattg tattcagaga atgagtggat ggtgaaaact cattacaatg gtttttgaat
     1741 ctactctaga gcttctagtt aaagctagac gtttacctac gttataggtc tgagaggatt
     1801 atacatttta gtatagtcaa ttagggtggc aacgcggata aaagattttc gtccctttta
     1861 tagggtaaac ggaggtcttt ttttattttt aaaatttgtt taaactaaga gtttacaagt
     1921 ataatgtttg taaattaagt tggaattata acttatatta tcaaaattat taggaggaat
     1981 taatatgtta gatattaaaa gaataagaga aaatctagat gatatcaaaa aagctatgga
     2041 aagaagaggt gaaagagaat ttgaccttga tgctgtagtg gaattagata ataaaagaag
     2101 agaaatactt caagaagttg aagttatgaa aaatgagtta aatgtaaatt ctaaaaaaat
     2161 acctcaatta ataaaagaag gaaaagatgt aacagaagaa aaggcaagat tgaaagagtt
     2221 atcagataag ataaaaggta ttgatgagaa agtaaaagaa gtagaagcaa aaatggaata
     2281 tacattaatg agaataccaa atgttcctca tccagaggta ccacaaggtg aaacagatga
     2341 agacaatgtt caaataagaa cttggggaga acctactaaa tttgattttg agcataaagc
     2401 gcattgggat ataggtacag gtctaggtat tttagatttt gaaagagcag ggaaaataac
     2461 tgggtcaagg tttactttat ataggggatt aggagctaga ttagaaagat ctttaatgaa
     2521 tttcttttta aatactcata cagctaagca tggctatacg gaagtcttac ctccatttat
     2581 ggccaataga aatagtttta tagggactgg tcaacttcct aaatttgaag aagatatgtt
     2641 taaaatagaa ggattagagt atttcttaat accaacagct gaagttcctg ttactaatat
     2701 acatgctaat gaaatattag atgttgcaga gcttccaatt aaatactgtg catatactcc
     2761 ttgttttaga tctgaagcag gttcagcagg aagagataca agaggtttag ttagacaaca
     2821 tcaatttaat aaagttgaat tagttaaatt tgttaaacca gaagattctt ataatgaatt
     2881 agaatcatta actcatgatg ctgaaactat gttacaaatg ttaggtcttc catatagagt
     2941 tgtaaaaata tgtacaggag atttgggatt tacagctgca tttaaatatg atttagaagt
     3001 ttggatgcca agctacaata gatatgtaga aatatcttct tgttcaaatt ttgaagattt
     3061 ccaagcaaga agagcaggaa taagatttaa aagggataaa aaatcaaaag cagaatatgt
     3121 tcatacatta aatggttctg gattagcgat aggaagatgt ttggctgcta tattagaaaa
     3181 ttatcagcag gcagatggtt ctgtagtagt tccagaagaa ttaagacctt atatgggtgt
     3241 tgatgttata aaataaattt atattaattg tatatgtaga gtatgctttg atgaagagaa
     3301 tatattatga gtaaacaata aataaaaaaa ctatgaaatt gttataaata ttaatttcat
     3361 agttttttta tgttaattta cacagcagaa ttttcatcat aaaacactaa atatttttat
     3421 aaaacactct tgtatgaata tctaaaaatt attttagaac taatttaaaa aaaatatagc
     3481 tatatatagt aataattaaa taaacaaaat aaaattaagt tgaattataa aatgaattat
     3541 gataagataa gtttgttata agttttggga ggaattatgg aaagttcatt ttatatgaaa
     3601 gaagcattaa aagaagcata taaagcatat aataaaaaag aaacaccaat aggtgctatt
     3661 atagttaaag ataatcaaat aatagctaga gctcataacc ttacagaaac gttaaaagac
     3721 tctacggcac atgcagaaat tttagcgata aaacaagcat cagaaaaatt aggtggttgg
     3781 aggttgactg attgtgattt atatgttaca atggaacctt gcataatgtg tagtggcgca
     3841 atagttaatt ctagaataaa aaagcttata atagggacaa gacatgtaaa aaattcatat
     3901 atagaaaaac agcatgagtt taaattagat tattttaata ataataatgt aaaagtagct
     3961 tttgatgttt tgcaagaaga atgctcgatc atattgcaag aattttttaa ggcattgaga
     4021 aaaagagatt aaggagagat ggtcgagtgg tcgaaggcgc tcgcctggaa agcgagtata
     4081 tgggtaaaac tgtatcgtgg gttcaaatcc cactctctcc gccagaaaca atgaaagtaa
     4141 ttaaatatgg ttgtaatttt gctgtactag atggggaggt agcggtgccc tgtaacctgc
     4201 aatccgctat agcaggattg aattccatct aaaggcttat tttctgtagg gccgccctaa
     4261 ataagtggtg ttgatgctta ggtcctgcgc aatgaaagct catgaacccc gtcagatccg
     4321 gaaggaagca gcggtaagtg atcacttttg tgtgccgtgg gggtgcctaa gttgagctaa
     4381 ctgttttggt aacgcctatg gaattaagtc gatagatggt gtacagcatt taatatataa
     4441 aagactaatc ccttagataa tgagggattt ttttattaat tttgctaaag agaggtaaaa
     4501 agatgcataa agcattatat agagcatata gaccacaaaa atttgaagat gttattggtc
     4561 aagatcatat tattaagact ttaaagaatc aaatatatag tgataatata ggacatgctt
     4621 atttgttttg tggaacaaga ggtactggta aaacatctac tgcaaagatt ttttcaagag
     4681 cagtaaactg tttgaataaa ataaatgaag aaccgtgtaa tgagtgtgag atatgcgaat
     4741 ctgtattaaa agataataca atggatgttg tagaaataga tgcagcttca aataatagtg
     4801 tagatgatat aagagagctt agagaaagtg tgaaatattc tccagccaat gctaaatata
     4861 aagtatatat aatagatgaa gttcatatgt tgtctcaagg agcttttaat gcattattaa
     4921 agaccttaga ggaacctcca tcatatgtaa tttttatatt agcaactaca gagcctcaca
     4981 aaatacctgc aaccatttta tcaagatgcc aaagatacga ttttaaaaga gttacagtta
     5041 aagatatgac ccttagaatg aaaaagattt gtgaagatga aggaattgat atagatgata
     5101 aagctcttaa tttaatagct agaaattctc aaggagctct tagagatgca cttagtatat
     5161 tagaccaatg tatgtctttt ggagaaagta agatagatta taaagatgtt gttgaattaa
     5221 tgggaagcgt aaatatagaa caattatttg aattgtctca atgtattgta gagcaggaca
     5281 ctaaaaagtc tcttgagata ttaaatgaat ttgtcttatg gggcaaagat ataagaaatc
     5341 ttataaatga cttaattgat cattttagaa atttaatggt atgtaaggtt tcatcagaat
     5401 tggatgaaat aatttcatta ccagaagaaa ctatagaaca acttaaaatt cagtctaaaa
     5461 atatagatat aaatgattta ataaggattt taaatatatt atcaataact caggatgata
     5521 taaagtcatc ttcaaatcca agggttttag tggaaataac cataatgaaa attgctcaac
     5581 caatgtttga tgaaagtaaa gaagctctta taaaaagagt tgagaactta gaaaaaatga
     5641 ttgaactggg gaactttaag tctgaaaaaa ttgggaataa taaagaaaaa gagtatgagg
     5701 tcgatagaga aatagatgta aaacaagaaa atgtagttta tgaagatgta aaaaatgagg
     5761 acgttatact tatagagtca tcttggaaga atatacttaa acaaattaaa aaagataaaa
     5821 aaatgccaat atacgcactc ttaagtgaag taaaaagttt taatgtatat tctaatatgt
     5881 tgtatgttat atttgatgat aaatttgatt ttgcaaaaac tagactgagc tctcaagata
     5941 caataaatta tttagaaaaa acaataagag atgttttaaa tagaagtttt aatgtaaaaa
     6001 tagtactgac atcagaagtt aaagatataa atcttgaagt taaagagaaa aaggacattg
     6061 gtgaggaaat attaaagaac atagtgagtg aagagatatt agaaataaaa gatagtattg
     6121 atgaaaatga gagtaaataa taaaatacta ttatgatata ataataaaat gaaaataagt
     6181 ttaggaggaa tagtgtttat ggctaaaaaa ggttttggag gcggaatgat gcctggtggt
     6241 ggaaatatga acaacttgtt aaagcaggct caaaaaatgc aagaaaatat gcaaaaagct
     6301 caacaagagt tagaaagtaa agaagtagaa gcttcagttg gtggaggagc agttactgta
     6361 aaagttaatg gtaaaaaaga agttatagat ataactatca agccagaagt agttgatcca
     6421 gatgatatag aaatgctaca agatttagtt ttaagtgcag taaatcaagc attgagaaat
     6481 attgatgata tacaagcaag tcaaatgagt aaggtaactg gaggtatgaa tatacctggg
     6541 ttattctagt aggtggtatt tatgcaagtt tatacaggtc ccataacgag acttatagag
     6601 gaattttcaa agcttcctgg agtaggaaga aagactgctc aaagattggc ttttcatatt
     6661 attaatatga atactaatga tgtggaagct ttatctaaag cgattataga tgcaaaaaga
     6721 gaaataagat attgctcaat ttgttgtaat ataacagata cagacccatg tagcatgtgt
     6781 tcaaataaaa gtagagattc aagtgttata tgtgttgtgg aagatccaag agatgtagca
     6841 gctatggaga gaacgagaga gtttaaagga cagtatcatg tattaaatgg agtaatttct
     6901 ccaatggatg gaattggacc agatatgtta aaaataaagg aacttatcca aagacttggc
     6961 aatcaggaag tcaaagaaat aataatggca acaaatccta ctattgaagg tgaagctaca
     7021 gccatgtata ttgctaggtt agtaaaacca atgggaatca aagtcactcg gatagctcat
     7081 ggtcttcctg ttggaggcga tttagagtat gcagatgagg tcacaatatc aaaagcacta
     7141 gaaggcagaa gagaaatata aaaatcgagg ttaatacctc gatttttgta attttttgag
     7201 ttaaattcaa attaattttg atttagattt attgaataaa tttcttctgt attgtataat
     7261 ttgttgtata aatcatttaa aaattttgat aggggtgcaa catgaaacca attatgaact
     7321 tagaagaaca taatgcaatg aaagaaaaaa gagttaaaaa aatgaaatca aaagataaac
     7381 ctaaatatga tgattttgag gatataagtt tttctaataa gaaaaaaact aaaaaaaatg
     7441 tacgaaataa aaatgctgaa tttagaaata attataaaaa ttatcagtat gattatgaag
     7501 aagactttta tgaaccaatt taatattaaa taattagaaa
//
LOCUS       DAKBNN010000188         7460 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000188 DAKBNN010000000
VERSION     DAKBNN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.191"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            403..1269
                     /locus_tag="OUB02_003193"
     CDS             403..1269
                     /locus_tag="OUB02_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT8886922.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1511..2320
                     /locus_tag="OUB02_003194"
     CDS             1511..2320
                     /locus_tag="OUB02_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886923.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2464..3528
                     /locus_tag="OUB02_003195"
     CDS             2464..3528
                     /locus_tag="OUB02_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCT8886924.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSIKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3556..4275
                     /locus_tag="OUB02_003196"
     CDS             3556..4275
                     /locus_tag="OUB02_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8886925.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4299..4883
                     /locus_tag="OUB02_003197"
     CDS             4299..4883
                     /locus_tag="OUB02_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCT8886926.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            4885..6234
                     /gene="pabB"
                     /locus_tag="OUB02_003198"
     CDS             4885..6234
                     /gene="pabB"
                     /locus_tag="OUB02_003198"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HCT8886927.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTSIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6224..6967
                     /locus_tag="OUB02_003199"
     CDS             6224..6967
                     /locus_tag="OUB02_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HCT8886928.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYTKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDVPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            7001..>7460
                     /locus_tag="OUB02_003200"
     CDS             7001..>7460
                     /locus_tag="OUB02_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886929.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDV"
BASE COUNT         3091 a          662 c         1389 g         2318 t
ORIGIN      
        1 attataaaaa gaaaaatttc atttaggaat taattataaa gagacaatta ctaaacttga
       61 atttgctaaa ataaaataaa ttactgcgaa aatagatatt tagaaaaaaa tactataaat
      121 ttactatatt gcaagttaaa taataagcag tatcatatta tgctagaaaa agtttcattt
      181 aaggcataaa tgagatgatt tgaacgtttt taaagattag aatataaaag attgcactag
      241 acatatatga aaaagataat ataatagatg atgtaataga tgacagttca agaaattata
      301 aaaaaaattt atttgaaata ttatctatta catcaggaat aatatatgta agagtaagct
      361 tttaaaattc taaagattta gaaagattga agggagaaag aaatgagtga agttaagaat
      421 accctacaaa aacatccagt tttaagatat tcaacaatgc tatttgggat gttgatgaca
      481 tctataggaa taaatgggtt cttaagacca gcacatctac ttagtggagg tgctacaggt
      541 atagcaacat ctataaacta tttgacaaat ataaatgtag gtttattaac atttttaatc
      601 aatattccaa tatttatctt agggttcata taccttgaaa aagagttttg tatttcaagt
      661 ttggtaaata tgattgtatt ttcattatta ttaggagcaa ctcaggaaat aagtaatatt
      721 atacctatac atgatatatt gcttcaaagt gtatatggtg gtatattatc tggtttagga
      781 gtcggagttg tatttagaac taggtcttct caaggtggta cagatataat agctgcaata
      841 ctaaaaatta agaagaatat agaaatgaaa gacactgctt tagcaataaa tggcttgata
      901 gtacttacag gaagtttttt atttgggttg gatttagcat tatacactct tataggtcta
      961 tttctaaatg cgtattcaat gagttttatt aaagatgcaa tgaattacca aaaatctgtt
     1021 atggtaatgt caaatgaagt cgatttaata gcagaagata taatgaagag tttagttaga
     1081 ggtgtaactt ttttagatgc agaaggtgca tacactcatc aaaaaaagaa gattatatat
     1141 acaatagttt catcaaatga gataccaaag ataaaagata tagctttgaa gtatgataaa
     1201 aaggccttta tatctgtaaa tgatgttaca gaagtaaagg gaagagggtt taaagcaaaa
     1261 gatttataga aaattttaca agctgggagt ttataatgta aatttgtata ttatatctct
     1321 cagcttattt aaatttagta tacgaaaata aaggttttat taatgatttg tgtatcactt
     1381 taaaagatat cattttaaaa ttaaaattga aaaaattact taatatgtag aaaacatgac
     1441 cactattagg atataataaa tttaatagat ttagtaaaat atctaggaat aacgttggag
     1501 gaatggcatt atgaatttat ctaaagaata tgttaatttg tatgataatt atttgaaata
     1561 tataaatgaa gtaaacaaag agattagaag tataaagaac atgaagaaac gtgttttaaa
     1621 aaggggcgat tttgctccag aaaatgaagt attagaattg gaaggaaaaa caatacgcga
     1681 tatagatgat gtcgatacaa aaaaaccaaa aaataaaatc tataaatttt caaatgggga
     1741 tgtatatgta gggaaattct tagagggaaa gatggagggg aaaggctcat atactttttt
     1801 tgtagatgaa ggaacagtta tggagtacat aggagaattt aaagaagata aaaaaaatgg
     1861 aaaaggaaac tttatatttt caaatggaaa tgaatatata ggttattttg aggaagatat
     1921 gatgagcggt attggaaata tgttatatag tagtaaagat gaatatattg gaacttggaa
     1981 aaacggaaaa aaagatggaa aaggtattta taagtggagt gatggatgta tatatgcagg
     2041 tgaatttaaa ggtggaaaga tggaagggta cggtatttgc tataatagta aaggggaagt
     2101 actttatgaa ggagaatgga agaataattt gattcatggt aagggaactt atatatggga
     2161 aaaaggtaaa aagtatatag gtgaatttat gcatggtaaa aaacatgggc aaggaacttt
     2221 ttatctaaat aatgaattgg tttatgaagg gacttggaaa tttgataagc catctatatt
     2281 tgatagaaca ttagatgaaa tattttcctt gagactgtag ctttatctaa ttagtagata
     2341 aagctttttt ctaatactta ctgtaaatag gtatgaatga tattgattag tatacactat
     2401 caatattaac ttaataaatg caatattaaa aaattttgat tgattttaag aaggagagat
     2461 atattgaaga gattaattgc tttagtgtta gtagtactta ttgttttatt ttatagtcct
     2521 gaattattaa aagaaggata tgatttaaaa gaaaagtttt ttcaaagtga aatatggaat
     2581 gaatttttag acaaaataaa tataaatgat aattctaaga aagataaaaa agaaaaaagt
     2641 caaaatttac aatcaaataa tactgaaggg gagaacttag gagagtcaga tataaagaat
     2701 tttgaaaaaa taagcttagg agataattta agctatgttt tatcaagtat tggtaaacct
     2761 ggtagaattg atattagtga atatggtttt gattggtacg tatataatca atatggaaaa
     2821 gaatttgcaa tggttggact tgaaaatgat gaagtagtag cactttatag taattctatt
     2881 aattcatgtg aaaatcaaga tataaaacta aatcaagata gacaaacagt gagaactaag
     2941 attacaccat taaaatataa gagaaaagga aataccagat atataataaa ctcagaaaat
     3001 caatatgata taataagtaa ggaaggtaag tatattacaa ttttctacga tattcatgaa
     3061 gaaaatagag tctgttcata tttaataatt gataaaagca ctgaagatga gtttaaaaat
     3121 ttatatccag atgatagcga agaacttaaa aaatgttttg aacttgaagt tatagacctt
     3181 gtaaatagtg ttagaaatca aagagggtta aactcattga gatacagtga acaggcaact
     3241 ttgagttcaa taaaacatag tgaagatatg agagataata atttttttga ccatgtaaat
     3301 aagaagaatg aaactccatt tgacagaatg aaaagagaag gtatagtata tacttcagct
     3361 ggagaaaaca tagcagcagg acagattaat gcaatatatg cgcatgaagc ttggatgaac
     3421 tctgaaggac atagaaaaaa tattttgggg aactataata atattggagt tggagtaata
     3481 tttggaggaa gctataaaac atattacact caaaattttt ataaataact aaaatatttg
     3541 aaaggaagtt aaaaaatgac atcatggaaa aaaaagacag tatataagtg cttaatagca
     3601 gtagtcttgt tttgtggaat agtgttaata tctaatttta gtaaagtttc tgctttgatg
     3661 atggatacta atggaaatgt tttgattaaa catggttcaa gggaaaaaaa gctaatagca
     3721 attacctttg atgatggtcc tcatcctaaa gaaactagtc aagttttaga tgtattaaag
     3781 aaatacaatg taaaagctac tttttttata gctggaaaac atgcaaaatg gtataaagaa
     3841 ccattagtaa gagcaagtaa agaaggtcac gagataggaa atcatacttt caatcatcct
     3901 gatataagta atttaagttc tagtcagata gaagaggaga ttgttaagtg tgaagacatt
     3961 ttgaaagaag taactggaaa aaaaccgaca ttatttagac caccatttgg aagttataga
     4021 gaaaaagaat taatagaaat tgcaaaaaag catgactata aagttgtttt atggacaggt
     4081 gtagatgtga aagattggaa aaatccagga gctaatagca ttgcagataa aataattaat
     4141 aaagtacaaa atggagatat aatacttttg catgattatg caactaatga tacagttgaa
     4201 gctttagata tgtttattcc aaaaatgatt gaaaaaggtt ttaaatttgt aacagtttct
     4261 gagttgataa aataattttt aggggggaga tttacagtat gattcttatg atagacaatt
     4321 atgattcttt tgtatacaac ctagtccaat atatagaaga attaggggaa acagttgtag
     4381 taaaaagaaa taatgaaatc aaaataagtg acattgaaga actaaatcca gaggtaattg
     4441 tgttatctcc aggaccgtgt tctccaaaag aagctggtat ttgcatagat attgttgagc
     4501 attttaaggg gaaaaaacct atactaggaa tttgtttagg tcaccaaact attggacatg
     4561 tttttggagg agatattata aaagcacaac agcctgtgca tggaaaagta tatagtatca
     4621 accatactaa taaaggagtt tttaggggac ttaaaaatcc tttaaatgtc actaggtatc
     4681 attcactaat agttgactcc aatacagttc caaaagaatt agaaattaca gcaattactg
     4741 ataaagggga aataatgggg attaggcata agaaatattt aatagaagga gtacagtttc
     4801 atccagaagc tattttgtca gagtatggtc atgaaatgct taaaaatttt ataacagaag
     4861 ctagagaaag ggtacatatg taatatgata agagagataa atactaaatt aaattctttt
     4921 gagatattta ctatttttag aaatgaacat gacagtttta tattagacag tgctatggat
     4981 aaagaaaaat tgggaagata ttcttttata agtagccaac catttaaggt attaaagtat
     5041 aaagatactg atgaaaatcc tttagaagtt ttaaaagaag aactacataa gtatagggtt
     5101 gtaaatgata ctaatcttcc atttgtagga ggtgctgtgg gatatttatc ttatgattta
     5161 ggtaattaca tagaaaattt acctagaact gctgtagatg atattgagat gcctgatatg
     5221 tattttggat tttataatca tgtaatagtt atagaccatc ttgtgcaaaa aacttatata
     5281 gctactccaa acatagatat cgaattagaa gaaaagataa tagatgatat agaacaaaga
     5341 atattaaaag aagagaaaaa aggtatagat agcatatgtt atgaagaaaa agaagtaaca
     5401 tctataagac ttaaatctaa ttttacaaag gaagaattta aaaatgcagt tcaaagtgtt
     5461 agagagtaca taagacaagg agatatatat caagctaatt taacacaaag gttcagtgga
     5521 gaaacagaac ttacaagctt tgaattgtat agggacttaa gaagatttag cccagcacca
     5581 tttggggcat tcttaaactt tgaagatgca catatattat caaattctcc agaaagattt
     5641 ataagatgtg ttaataagag aatagaaact agaccaataa aaggtactcg tccaagaggg
     5701 aaagataaag aagaagattt aagacttcaa caagaactta gaaatagtga aaaagataga
     5761 gctgaactac ttatgatagt agatttagaa agaaatgata ttgggagaat atctaaaact
     5821 ggaagtgtta aagttccaga gctatttgta attgaaccat atgcaaatgt aaatcactta
     5881 gtttccacag ttgtaggtga gttaaaagat gataaagatg ctactgatgt aataaaagca
     5941 acttttccag gggggtctat aacaggagca cctaagatta gagcaatgga aattatagac
     6001 gaattagagc caacgcaaag aaatgtatat actggttcaa taggttatat aggttttaat
     6061 ggtgatatgg attttaatat agcaattaga acaataatca aaaatgacaa aaaagtctac
     6121 tttcaagttg gaggaggaat gacttgggat tctgacccag atgaagaata tcaagaaaca
     6181 cttgataaag ctaaatccat aatgaaggct ttgaggggat attatgaaga ataatgtagg
     6241 ttttgatagt agtttaagta aatttggaat tggtttgttt gaaactatta gagttaaaaa
     6301 tggaatagca gtagatttag atatccatat agaaagaatg ctaagctcaa taaattgttt
     6361 ggatttagat ataaactata caaaagattt cttgataaat gaaattgtta catatataaa
     6421 aaaagaaaat gtcattaata aagcacttag aattactgtt tttgatgaag gttataatat
     6481 atctattagg gatgttccat ataatcaaga gacgtatgag aaggggttta aattagctat
     6541 atctccaata gttaggggga atagtttaat tcatagacat aagactacta attattttga
     6601 aaacatatat accaaaaata tcgcaaataa gactggttat aatgatggta tatttttaaa
     6661 ttcagaaggt gtaatattag aatgttctat gagcaatatt ttctttataa aaggagaaag
     6721 agtgtacact cctagtgata gattaccgat attaaatgga ataataaaaa aaaggattat
     6781 agagatttgt gacgaattac atatagagtt gatagaaaat gaaattaata tatcagaaat
     6841 tagtagtttt gattttgttt ttgtcacaaa tagtcttatg ggcgctataa aggttacaga
     6901 aattgacaaa ataaaattta ataaagaaaa tgttgttttt gataaaatag tgaagttatt
     6961 agaataaatt aaaatattaa caatttgttg gagacaaaag atgataggtt gtatttatga
     7021 agtatacaat gaagagataa ctaaaataat agaaaaccaa aacactttgg ctgtatattt
     7081 agtgggttct agtaaagacg tagattttac tctttatgat gtagagataa acgatattga
     7141 tgtgttcgtt tttgtaaaag aaggggaaaa acaagagaga atactgttta aaaaaaaggg
     7201 aatagaattt gatgtaaatt acttttcaaa agatggtgta aaaaaattat tatcaaatag
     7261 ggaatatttc tttgtgaaag aaatgaaaga tgctaaagta ctgtttgata gggagaatat
     7321 atcacatatt ataaaagata taagtagaaa tatttactta gaaggaccca gtaaaatgtc
     7381 attagaggaa aagtctttta taaagcaaga tattggagca aaaatatcga atttaaaaaa
     7441 taaagaaaaa tttgatgttt
//
LOCUS       DAKBNN010000189         7334 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000189 DAKBNN010000000
VERSION     DAKBNN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.192"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            135..3314
                     /locus_tag="OUB02_003201"
     CDS             135..3314
                     /locus_tag="OUB02_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT8886930.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMKLENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3599..4363)
                     /locus_tag="OUB02_003202"
     CDS             complement(3599..4363)
                     /locus_tag="OUB02_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8886931.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4519..4752)
                     /locus_tag="OUB02_003203"
     CDS             complement(4519..4752)
                     /locus_tag="OUB02_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886932.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPSTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4826..6319)
                     /locus_tag="OUB02_003204"
     CDS             complement(4826..6319)
                     /locus_tag="OUB02_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT8886933.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(6647..7189)
                     /locus_tag="OUB02_003205"
     CDS             complement(6647..7189)
                     /locus_tag="OUB02_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8886934.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2688 a         1063 c          815 g         2768 t
ORIGIN      
        1 tagtaaattg ttatttacac atatattgtg atagataaaa gctattcata tctgccttaa
       61 ttttagtctt aaatttagag tgtaaattga atgcatttag attataatat atacagtgag
      121 gtaaggtgaa taatatgaaa atagatatga tatataaact aataagaaga aatacagaac
      181 catcaagatg gtcaagaggt gtaaaatatt atactaatca attagttgat tatatagaca
      241 tagatattga tgacctacaa aataacctgt tgacatttcg tggtggagtc aattctgaag
      301 ataattatag cttttatttt aattcaataa ctatagatat aaagcacttt aagataattg
      361 aaaatgattg tagttgcgct gactgtcaat caaagggcaa aagtattttt atatgtaagc
      421 atatagttgc tattgtgata aaaagcttaa aacaaataaa aaataaaaaa gatgttcctc
      481 ctattctaat agatatatta ggtgcagata aggataagct ttctaataca aattctaaaa
      541 ccagcttaga tttaagcaag caattacttt ctttgttaga caatactaaa agagaaaggg
      601 ttaatttaga tgtattgata aatgtggatt caaataaagc aattgctgaa tttaaaatag
      661 gaattgataa aatgtacatt ttaaaaaaca ttactgaatt tgcatattca aaacttaatg
      721 gatatgaatt agattttggg aagaaattta cttacaaccc caacactcat tattttgctg
      781 atgaagatga gaagttagta gatattatag aagaatatgg gacaaatctt tcatacaact
      841 tttcttcatc acattcaaaa aaatttatgg agataaatgc aagctcttta aaacaatttt
      901 taaatgctat taagtacaag gattttaccc ttattttttc taaagaggaa tatcatccaa
      961 agatattaaa tgatactcta cctgtaaggt ttgattttaa aaatatagaa gataaaatag
     1021 tcttatattc taaagattca ctacctgttc cacttactaa aaaaaatgat gtacttttat
     1081 atgatggcaa tatctattta ttatcacata aaaaagctca tgattattca aaaatatatc
     1141 aaatattatc aaagactaaa gagttaaaat ttgataaaga ggattctaag gatgttttag
     1201 gattgctagt tcctagatta aaatctatat ctcaagaagt tcatttagat gataacataa
     1261 aaaataatat aacaaaagat tttaaatctg agttctactt tgatatgatt gatggcaaca
     1321 tatgttgtga tgtaaagtat atatatgatg atgaagataa aaaatttgtg ctccccaaca
     1381 tacaaaagga agccactatt gaaaataaac ttacttcttc acaatttaca aaggaaaata
     1441 atcattatgt gtttaaagga actgaccatg acctatttac atttttagat ttacaattag
     1501 aatctctaaa ggaatttgga gatatatatt attctgagaa gtttaaattg aaaaaaatat
     1561 ataacgcttc atcaattcaa gcttcaatca atcagactaa ccagaattac ctagaattta
     1621 attttaatat atctgatata aatccaaaag agtacaagga tattttgaaa gcttttcaag
     1681 aaaaacgtac tttttataaa cttaaagatg gaaactttat agatttatca gaaagagaaa
     1741 caaaagattt ttttgaatta gttgaaaatc tagatataat ggataaatca aaaaataata
     1801 aaattcataa aaataaagct ctatatataa atgatgtaat acgtagtaaa aatttaaagt
     1861 ttatagatgg taaaaaagaa ctagacagta tatgtgaaaa atttagaaat tttgatagca
     1921 taaatttaga aattcccaca aatttaaatg caaaccttcg tgactatcaa ataaatggcc
     1981 tcaattggtt taaagtttta gattattata aatttggtgg tatattagct gatgaaatgg
     2041 gtcttggtaa aacaatacag actattgcat ttttattatc attatctaat aaaaaaagtc
     2101 ttattgttac accgacatca ctcatttata attggaaaaa tgaatttgaa aagtttgctc
     2161 cagatataaa agttttattg atacatggaa ataaaagaga tagagaaaaa tgttttatga
     2221 aattagaaaa ttttgatgta atacttacaa catatggtac tcttagaaat gatttagaca
     2281 aatatagtga aataaagttt gattattgta tattagacga agctcaaaat ataaaaaatc
     2341 cagtagcttt agtaactgag tctgtaaaaa gtataaatgc agaaaataaa tttgcactca
     2401 ctggtacacc tatggaaaat aatctactag aattatggtc tatatttgat tttataatgc
     2461 ctggctatct atatagtaaa gcaaagtttc aggaattgtt tataaataaa gaagataatg
     2521 ttaaaaacct gaaaaaattg ataaaacctt ttattcttag aagaagtaaa aaacaagtta
     2581 tgaaagaact tccagataag attgaaaaaa acttttttgt tgaattgaat aaagagcaga
     2641 aaaagatata tagtgtatat tcaaaagata tacaagataa aatgaaagat aaaaatctaa
     2701 agaaagataa aatagttata ttttcttatt taactaaact aagacaactt tgtttagacc
     2761 caagtatagt agtaaaagat tacaataaaa aaagttcaaa aattgaaaca tgtttagaaa
     2821 tacttaggga tagtataaac gaaaaccaca aaatattatt gttttcacaa tttacttctg
     2881 tgcttaagaa cataagtaag gaacttgata agtataaaat taaataccat tatatagatg
     2941 gtaaaactaa tgccaaagaa agacttgaac ttgtagatga gttcaataat agtatggata
     3001 aaaaagtttt tttaatatct ttaaaagcag gcggaactgg tttaaatctt acatctgctg
     3061 acatggtaat tcactttgac ccatggtgga atccatcagt agaaaatcaa gctagtgata
     3121 gagctcatag atttggtcaa aaaaattcag tacaagttat aaaattgatt gctaaaggga
     3181 ctatagaaga aaaaataata aaattacaag aaagtaaaaa agagcttata aatcaattta
     3241 taaatggaga actttctaat gaaggtgtat taaaaagtct atctgatgag gagataataa
     3301 acttatttaa ctaaattatt taatcctaca gcttaactat ataaatttgt tctaacattt
     3361 atatagttaa gctaaaatat ctgaataaat aaaatttaat attgttattt aatcccaact
     3421 ttacaatcta atgatatctc tttctataca tattgcatat acaaatgtaa taacttgact
     3481 attagaatat tttgttttaa caatactatt tcatcataat aatgaagtat atgtacactc
     3541 acttttgtaa gttttgcaat ccattttaca gttttcatta ttgtaaattt tggttaaatt
     3601 attcctctaa aaatttcttt gctacatttg aaaaatctaa tccattcgat aaaattgcag
     3661 gatgaccacc tttatcaaac aaatacattt taccattttt aatcttttca agaagtaatg
     3721 aaaaagtatt ttcaaaaaaa tcactagata ttagagatac aaactcatct tctctacttc
     3781 ctgttaaaag cacctctggt tgcatagttt ctagaggctt atgaaaaaac tttcctatag
     3841 ttttataatg ttcaaaaata gcttctgtat cattatctac aactttttcc cagttttctc
     3901 cttgattata aatataaaaa gcttttgcac catcatcttg cttagatgct tcacgctcta
     3961 ttttcacatt ttgtacaaat gactctagag gaacttctcc ttcaaaacta tctgcaatta
     4021 ccttattcac taaatcaggt ctctcaagtg caacatttaa tgcaacaaga gcgcctccac
     4081 tactaccaat tatgtttact tttttatagt tcattgcttc taaaaaacat atcacttgca
     4141 tagcctcatc aaaccataag tcagcagaaa atttatcgac catttgagat tttccatgac
     4201 ctacaaaatc aattaatatt actttatatt catctttata aaaatcgatt atatcattaa
     4261 acatcttgga agaggctgta tttccatgta aaaaaagtaa tggtgtacca tccccaattt
     4321 cattatagta gcactgtttg tcgttatagt taaaatatgc catatatttc ccccctttaa
     4381 tatccctata ggtatatatt tattacttat atttaaaatg actattccat tagccactta
     4441 aaaatagtat aacttagaat gtagataaaa tcaacattcc ttgctaattt atctattgtt
     4501 tgtctaattt attataattt aacttctgta aaatttcttt tttgtacgaa aatattcctt
     4561 ccatttacaa caatcattca aatgaagtat aacatgacga gtagaaggca taagcaaaat
     4621 tccagcaata tctttattcc cccaaaagtc caacaaccaa atagaatctt catgctttat
     4681 tactcctcca gtcaccaaaa tcctttctaa gtcaattgga gagacctttc gtttcatatc
     4741 ttcagctttc aaatccttaa caacttctat tgtgtttttt gcaacttcat ttcgataaca
     4801 aagcaattca tcaatattga ctaaattatc gtatatctct tttcacttta accatttttt
     4861 tctttatagg tatctcctta tattttttta atatcaaatc tcccttataa tttaatatat
     4921 ctacatatga acataaatct tgaacttcaa ttacacctat atcatcatta gttatacctt
     4981 ttatattact aaatgcacca actatatcta aaactcttat tttcttcttc ttaccagcat
     5041 ttaaatataa tttaacaact tcactttcta tatttagttt tatatcttta cttttgtaag
     5101 aatttttatc aacttttttt ctctttgata ctttttttag gattcctttt gaatattctt
     5161 caaacaaaac tttgccatgt gtaattctac ttttttctat atcttttaat tcatttattt
     5221 tatatcctat atacgtttct atattctcta tatatttttc atccttttca gttactatag
     5281 taattgcctt tccactattt ccttttctcc cagttctacc tattctatgt atatagtttt
     5341 ctttatcttg tggcacatca taatttatta ctaaagaaat atcatcaata tgtattcctc
     5401 tggacgctac atcactacta actaatatat taaatttttg gtttttaaaa tccttaatta
     5461 caaaaattct tctttcttgt gataaatctg catgtaactc tcttattaaa aaaccctctt
     5521 tactcatctt gttatataat ttagatactt tctctttggt attacaaaat attatcacac
     5581 tctttggatt ttcgctgtat attaattctt ttaaaaggat gtacttctct cttccatttg
     5641 ccttgattat tttgtcatct atttgtcttg ttttttgatt agtatcgctc tcattttctt
     5701 ctatatttat aacactgtaa tctagcatat atttttcaca tatatatttt atctcttcat
     5761 ctattgtagc tgaaaaaaga cttactattt tctccttagg taaatttaat aatatttttt
     5821 ccatttgttc aacaaaacct ttattaaaca ttttatctgc ctcatctatt actaaatact
     5881 ttacattttc caacttaata gaacctctat ttatatgatc aagtattctg cctggagtag
     5941 ctacaacaat atgaaccctt tgtttcaatt cagctatttg gtcttttata gattgtttgc
     6001 caaatatagc actacatctc accttcttta gtctccctat atctgatatc tcatctttta
     6061 cttgcaaggc taattctctt gttggaacca ctataagagc ttgaatatta ttgtaatcta
     6121 catttatatt ctcgcataaa ggtattgcaa aacttgctgt ctttccactt cctgtttttg
     6181 acctaactac taagttttga ccttttaata attttggaat aacttctctt tgtactctag
     6241 aaggtatatt atatcctaaa ctcttcaatg acttaagtat cttttcattt aacttaaatt
     6301 tttcaaatgt atatttcata tattaccacc ttattatatt tatcttatct atatattttt
     6361 ctacattata ttattataat acaaggttct actattttta tattatttat tagactgaag
     6421 ataaaaatat atactctata aacctatagt ttagtagcaa attaattaaa tttttatatt
     6481 taaatacctc cttaaatata ctgataaatc aatattttca aggaggtatc tcttgtcaat
     6541 aatcaaacta atctatcttt tgaaagttta aattatacta atacattgta tttttattta
     6601 tatctaatat taaaaggtat taatcaatac ctaaatcacc ataatctcac atatttatat
     6661 ctttattcaa tataccttct gtaatcaatc gtgctatata atcataacct gattcatttg
     6721 ctttgttaca atcattcaca ttatcacaga aaaaactttc aatctgtatt gcaactggat
     6781 ttgtatttct caatatatat aaattttctc tttttttagc tcctctattt tcaaataatt
     6841 ctccaagtct attagatact cttttagcat attctaatcc ttcagtgcca tagtaaagca
     6901 cttctgtacc acattgtgtt ccatcagctt tatttaaatg tagctctact aataaatcgt
     6961 atttaccact gtttactttt ggaactctat agaaaaattc ttctctttct gacataaagt
     7021 gcctttctgg acaaattatt acatctactg tattaccttg acttataagc atctcagcta
     7081 acataggagc taatttttta ttataattgt attcatcaac tataccattt acacctgtac
     7141 ttttaccacc ttttagtata ctatgaccta ctgttatgca tattttcatt tacttttttc
     7201 accttctttt aagccaattt ttatataata gtaatataat tttaaagtta ttactaataa
     7261 taatatatta attgaagaag gtaaaagttc tccgtgatta tcattttttt aacatatttg
     7321 tcttatttta tcat
//
LOCUS       DAKBNN010000190         7317 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000190 DAKBNN010000000
VERSION     DAKBNN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.193"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            424..1116
                     /locus_tag="OUB02_003206"
     CDS             424..1116
                     /locus_tag="OUB02_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886935.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1312..3372
                     /locus_tag="OUB02_003207"
     CDS             1312..3372
                     /locus_tag="OUB02_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8886936.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3578..3901)
                     /locus_tag="OUB02_003208"
     CDS             complement(3578..3901)
                     /locus_tag="OUB02_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCT8886937.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3894..4595)
                     /locus_tag="OUB02_003209"
     CDS             complement(3894..4595)
                     /locus_tag="OUB02_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCT8886938.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4873..6258)
                     /locus_tag="OUB02_003210"
     CDS             complement(4873..6258)
                     /locus_tag="OUB02_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCT8886939.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6366..6509)
                     /locus_tag="OUB02_003211"
     CDS             complement(6366..6509)
                     /locus_tag="OUB02_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886940.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(6704..7126)
                     /locus_tag="OUB02_003212"
     CDS             complement(6704..7126)
                     /locus_tag="OUB02_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCT8886941.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         2820 a          834 c         1025 g         2638 t
ORIGIN      
        1 gtagctatta ttgcagtgaa atgaatgttt ctaagtatat ttgggtggaa gaatggtgga
       61 agaaaattag tgaaatgtaa ttcttccacc attcttccac ctagaaaata gaaactgttt
      121 aataagaatt aatatacatt aattcagctg aatgcaatca aaatacttaa atgaaagtaa
      181 tctgaaaaca acataattca aatcaattta ttcaaataaa cctcaattta tcttaaatct
      241 taagaaatat ttaagaatta ggataatatt ttcttaagat ttatatttta ttatatagtt
      301 ataagggaaa gttacaaaaa tgtaagccaa agtaatataa caatttagtc attgttgaga
      361 aagggagtga aagatattgt ataatataga taagtattat tatacaatag aggaagtgaa
      421 aatatgtaca atatattagt agtagacgat gataaagaaa ttgttgattc gatagaaata
      481 tatcttaaat cagaaggatt taaaatatat aaagcatatg atggaatgaa agctttggaa
      541 ataataacag agaatgacat tcatctaata ctaatggata taatgatgcc taaattagat
      601 ggaatcaaag caactgtaaa aatacgagaa gaaagaaata ttccaataat tttaatttca
      661 gcaaaaagtg aagatacaga taaaataata ggattaaata ttggagctga tgattatata
      721 acaaagccat ttaatctatt agagcttata gctagggtta aatcaaactt aagacgatat
      781 gtaactttag gtaattatga agatacatgt aaagaaattt taaaaagtgg agctttggag
      841 ttaaatacac taacaaaaga agttaaaatt gatggtgaaa acataaaaat gactccaatt
      901 gagtataaaa taattgaact tcttttagaa aataaaggac gagttttctc aatagatgag
      961 atatacgaaa aagtatggaa tgaggaaagt tttaattcag aaaatacagt agcagtacat
     1021 atcagacgaa taagagaaaa gattgaaata aatccaaaag aaccaagatt tttaaaggtg
     1081 gtgtggggag ttggttataa gattgaaaaa ttttagtgga aaaattataa aagaaaagag
     1141 tttaatattt aattgcattt tgattttagt catttttaca tttacacaaa tgttaggaat
     1201 tttaatatgt tttaattttt tctctgagag tttaatttac tctatgggga ttatgctttt
     1261 cttaaacttt attttatata aaatactgac aatgtagatg aggtgggcaa cttggagaaa
     1321 tatagagtat taaagttagt ttcattaatc ataatatcta tctcatttcc attaactata
     1381 ttgttggtga caataggact tgctgagcct aaatacaata tacaaaaaac ttctacaaag
     1441 agtgggattt ttgatatatc tgatgatttt tatgatagta atatatttga cgaaggaaca
     1501 atggcaagtc tattacccaa gtttaagcta tctgtaggtg gtaaattaac tagtaaagaa
     1561 aaaggtaaat tccaacaaga aggtaaaagt atagatttaa gtagtagtga ttctgagtat
     1621 gataaaatca tgattaatga acaagcttct gaagaatatt taagttatct aaaaaatata
     1681 aaagtgtatg ttattaatac atacacagga gtattttata caaatacgtc atatgctagt
     1741 ttatatgatt ttaaacaaaa aactaagagc tattgtaatg tagagataat aaataagata
     1801 ggaaagagct catatattaa aacaataaat ggagaaaaat ttgaactgaa taatctgagt
     1861 ctggaactaa atgaagtaga tgaaggtttt gaggcatatg tatcttttcc agaagagcca
     1921 actatagaag atggaatagt atatactaat tttcaaatat ttaaacaagc tacagaaaaa
     1981 gtaagattat tttttgtagc tagtttaagt ttagcaatta tatgtttatt atctataatt
     2041 ttatatatca aaataaaagg tgaagtttta aaaagaaata atgtattttt actaatatac
     2101 agtaagatac ctatggaatt taatgtaata ttagtatcta tttcaattgc aattttaact
     2161 atatatgctc ttgctgtatc ttatcatatt gatgtaattg cattagtttc tttatcttta
     2221 ctgatagtat caaataatat attttttata aatgtgcaat tgagttattt agaaaataaa
     2281 atgtgtatat ttaagacaag tattatagta agagtgattt tatggttaat aaaaactgta
     2341 agggaggtca gtaacgctat aaagaaagta tctttagcaa ggaaagttat tgttatagtg
     2401 ataatatctt tagctttaat catggtaatt agcactcgaa gtataggtat gggattactg
     2461 ttttctatat gtattttttt aggatttaca atatatataa ctaaaagact ttcatatctt
     2521 agttacgtaa tagaaggtac agaaagaata aaaaagggag aattagatta taagataaaa
     2581 attactggga atgataattt tacgtcttta gctgaaaata taaataatat tggagaaggt
     2641 cttgatagag ctatagaaga gcaagtaaaa agtgaaagaa tgaagtctga ccttataaca
     2701 aatgtatctc atgatttgaa aactccactt acttctataa taaattatgt agatttaatt
     2761 aaaaaagaag aatcaataca accagagtat ataaatgatt atataaatgt acttgagtca
     2821 aagtcaaaaa gactaaaatt attgatagaa gatttatttg aagcgagtag agtaagtagt
     2881 ggcaatatag aattacaaat tagcaagatt gatttagtgc aactattaag acaaagtata
     2941 ggtgaacttg aagaaaaatt atctaaaaat aatttatatt taaaattaaa tgttccaaat
     3001 gataaggtgt atatatgggc agatggaaga agaatgtata gagtatttga aaatctttta
     3061 tctaatatag caaaatattc cttagaagaa actagagtct atattgatgt atatgactat
     3121 ggtgaaaatg taaaggtaac tatgaaaaat atatcttctt atgaattaaa ttttgaccca
     3181 agtgagatta tggaaagatt taaaagagct gatgaatcaa ggaatactga aggaagtgga
     3241 ttagggcttg ctattgcaag agatttagta gctttacaag gtggaaaatt ttatatagaa
     3301 atagatggag atttatttaa agcaaattta gagtttcaaa aatatgaaga agaaataaca
     3361 aatatggaat gagatagtat ttaagtatat atattttttt tactgtcgaa agcttttatt
     3421 ataatatatt agaatattta cagtagagaa tgaagagtaa aaacagagta actttaagtc
     3481 actctgtttt ttatttaaaa tcaataatat aacaacttaa attaaaattg tacacatagt
     3541 tttaaaataa cttatacaat aaaacattat ccaaatatca caagataatt ccaaataaat
     3601 atatagatat cactcctgta ataatagaaa taccaataga tttaaactta tatgcaacaa
     3661 gaagtacaat cataaaagat ataattttag ggttagctat agataaatat agactatctc
     3721 cattaaaaaa tacatcttta gcagttaagg aagtaagtat tgctacaggt ataaatttca
     3781 tgattttttt caaaaaatca ggcatctctt tttttgaaaa aattaacata ggaagcactc
     3841 ttggtagata tgtaacaatg tacattccaa ttataataaa aaatatataa ttattattca
     3901 taagattcct cctttttaca gtaagattct tctataaaga aaccaattaa agatgctaaa
     3961 attgatgcaa ctattattac taaattgctt ttgaataatg catataaaat aacagaaata
     4021 atgattgatg atataaatac tacaatataa atcttatttt ttatttgaga aactattaaa
     4081 gctataaaca tagaagtcaa tatgaagcca gaaatagaac tgtctataga tatactttct
     4141 cctaaaaatg ctccaataaa attagctaaa actgatgaaa ttaagcagaa gaaattaaga
     4201 taaatagcat ttctatcaga ccaatttcca gcagtaaatt tttcataatt aactgcaaaa
     4261 gtttcatctg ttatagtatg acaaaatggc aatataaatt ttggactttt attttttatg
     4321 tacatgttca aattagaact cattagaaaa tgtcttaagt taactataaa tgtagtcaaa
     4381 acaatagatg ttacacttgc accagtagaa atcattgaag ctgcaatgaa ttgagaactt
     4441 cctccaaata ctaaagttac cattagtgct atttgaaaag gtgaaagacc agatttttgt
     4501 aatagtattc ctcctactat acctataggt atataaccta agaatatagg aattgaagca
     4561 ataaagcttt cttttaattg ttgagttctt tccacaataa atcactcctt ttgtattaaa
     4621 tgtattaaag tattcgtaaa acagaaaaat agattaattg tataattgca attataatta
     4681 aaaataaatt tggattaata tataaaatac tatacaacaa atagataatc ttaaacggtt
     4741 attaaatttt atctatttgt tattatgatg taataataca tattaatctc tataagttaa
     4801 ttactatctg gtgtaatttt attttaacaa caaaaaaaaa tacacacaat taaaatattg
     4861 tgtgcaatac aattattttt tctcttctaa tagtcttttt aatacgctca aaccagcttt
     4921 aagttcttct gtactttctg tagcacatac agctattctt attgcagaaa ttggtttttc
     4981 ttttccaaca gcaaaacgtt cagaagcata gacctgtaca ccttctttaa gtgcttgtat
     5041 ctcaaatttt tctgccatta ttccatcagg tagaatcaac catctaaata tacattcttc
     5101 atttcccaaa agattatatc cagataaata ttgattaata atcttgtttc ttcttcttgc
     5161 agatttacgg cgtgcattaa ttaatttatc ggcttcacct gatgtaatca tgcgataagc
     5221 aatttctgtt aaaaaataag attgagatag attaatgttg tataatgcgc ttgataatgc
     5281 ctctcgatac tgtttaggtg tagagatata tgctagccgt aaactaggtg caataatttt
     5341 tgataaactc gtaatataaa ttgtttgatt gggcagatag gaagccactg gatttaaatg
     5401 tgtttcatta agtaaactat gaatagcatc ctcaataaca attaaattgt atttagatgc
     5461 aatatttgca atcatttttc gtccattttg tgacattata tgtgtagttg gattttgata
     5521 atctggtatt atgtatagac ctttaatatt ttcattttta caagcatata atagtccttc
     5581 ttcactaatc tcattatctt cttgtttaat tggaattagt tgaatcccaa gcattttagc
     5641 tgctgtcttt attccaggat aagttaatgg gtctacacct attctatctc catgttgaaa
     5701 caaaccagct aaaatagcaa caattgcatt ttgtccacca cttgcaggaa gtaagctatc
     5761 tgatgtagtt tcaagcccag ctttataaat taatttggca atagcttctt tttgccattt
     5821 agcaccatct ctttgtccat attggaatat agttttaaaa tctatttcct taattatgtg
     5881 tttaaacaaa gtattcattt cttctagcgt gaaatctgga ttcattgtgc ccatttcaat
     5941 aaatgtaatt ttattattac tggacatcaa gaatagattt gaacgtgtat cataagatac
     6001 aaaagttcca cttcctgtaa ctccacttat tagacctttt ttttcacaaa ttttaaaagc
     6061 tctagagata gtactcacat taacatctaa gaaatctgcc aattcacgtt ggggtggtaa
     6121 tttagtacct ggtaataaaa atccattgtt gatatcttgt tctagttgtt ctgcaagagc
     6181 cttatataat atttgacctt ttgaaggtct ctttggtttc cagttcatag gataattttc
     6241 aaaagaatta attggcataa gttttcacct cacattatta atttgatttt ttctttatct
     6301 aaaacaataa attctaaaga tacaaataaa aactgatagg aaataattct atcagttttt
     6361 gtcttttaat tgatttcaaa tcttcctata atttttatac cttctttatc ataaagaggg
     6421 attgtttttt taaatatatt aaaatttctt tttcttgtat ataaaacagc ttcttctggt
     6481 gtctttggtt gattatcaca atcgtacaaa tttctttttt ttaactaaaa gcaataatac
     6541 ataaaattat tattgctaat gatactctta atgtggtata tagtcatagt gtcacatgtc
     6601 tatcaatagt tttttcagta attattgtaa cgaatagtag gactcataaa atacttagaa
     6661 atatgttttt agataaattt taaatatata atctaactta tatctataaa ttaacactat
     6721 tattctccaa ttccatttct agttttttta taaatttatt aaattcttct ggttgccttt
     6781 ttccaaacaa tactcttaca agagctgaat tgatttttcc aaatatattc ctcttgctct
     6841 cactttcaat aaaaataagt tttgtatttt ctaagtcaat atgtactaat tcatatctag
     6901 taatagtact ttctctttta ttgcttgttt ttatctcata tactctattt ttttcgaaat
     6961 tagttatttc aaccttaaat cttttattat ttttttgctg tacaaatgtt cctactgctt
     7021 ttttctcatt aaaattagga aaatttagtc ttgcagattt tgtaaatata ttaaatatgt
     7081 cttttgcaga atattttaat aaaacttcag ttttaaatgt attcatatcg ttttcccttc
     7141 tgtatataaa tttaatattt ataagttaat ttttaagtta aaatagtata atatgccata
     7201 ttaaatcatg ttaaaactat ttatatagct aaaatcttac ctatataagt atgtatacta
     7261 tcatataaaa ataaaatctt aaaatagaaa gggttaaaaa cacagtttat tataatt
//
LOCUS       DAKBNN010000191         7194 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000191 DAKBNN010000000
VERSION     DAKBNN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.194"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            473..982
                     /locus_tag="OUB02_003213"
     CDS             473..982
                     /locus_tag="OUB02_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCT8886942.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1010..1204
                     /gene="rpmI"
                     /locus_tag="OUB02_003214"
     CDS             1010..1204
                     /gene="rpmI"
                     /locus_tag="OUB02_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCT8886943.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1260..1616
                     /gene="rplT"
                     /locus_tag="OUB02_003215"
     CDS             1260..1616
                     /gene="rplT"
                     /locus_tag="OUB02_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCT8886944.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1926..2471
                     /locus_tag="OUB02_003216"
     CDS             1926..2471
                     /locus_tag="OUB02_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886945.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2458..2631
                     /locus_tag="OUB02_003217"
     CDS             2458..2631
                     /locus_tag="OUB02_003217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886946.1"
                     /translation="MKGNKSDLHGVEPIYTENRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2906..4831
                     /locus_tag="OUB02_003218"
     CDS             2906..4831
                     /locus_tag="OUB02_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8886947.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYINEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDELTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5232..7136
                     /locus_tag="OUB02_003219"
     CDS             5232..7136
                     /locus_tag="OUB02_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8886948.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3028 a          714 c         1360 g         2092 t
ORIGIN      
        1 gtataatagt atattagagt acaatataga atttgttatg tagtatagta gtatattaga
       61 atacaatata gaatttgttg ggtagtatag tgttatatta gaatacaata taggatttgt
      121 tatgtagaat atatagagtc atgtagaata tacatatata aaaggatttt gggtataaat
      181 ttaaaaatta atagcaaaat gagtagtata gacatataat gagtagtagg aagtaattaa
      241 atttttccta aacaaaaaaa atagttgcaa ataatttgat taagtgatat agtaaataag
      301 gtaagaataa aacaaagaag aagtccgctt ctcaccttac gccggaggtt agtaaggtaa
      361 atataagtta aagcttaatt ttaatttaag tttgtttaaa tttggtaact tatattgcgg
      421 atgattacgt catccgcttt ttattttata aaaattttgg aggtgtccta caattagcaa
      481 agaattagca atcaatgaac aaataaaaga taaggaaata agagtccttt caccaacagg
      541 tgaacaactt ggagtaatgc ctactaaaga agctcaagca atggctaata gcaaaaactt
      601 agatttggtt caaatctcgc cgaatgcaaa tccaccagta tgtaagataa tggattatgg
      661 aaagtttaga tatgagcaag cgagaaaaga gaaagaagct aagaaaaaac aaaaaactat
      721 agttgtaaaa gaagtaagac ttagaccagg tattgaacaa aatgatttaa atacgaaagc
      781 taataatgct ataaagttcc ttaaaaaagg ggataaagtc aaagttgaac ttagatttag
      841 aggaagagaa ttaggacata aagacatagg taaagaagtt atgcttaagt ttttagatat
      901 aataaaagag tttggagaac caacaaaagc ccctgcattt gagggtaata atatggttgt
      961 aattatagat ccaaaaaaat agatgattaa ctgagaggag gaatttgtca tgcctaaaat
     1021 gaaaactcat agaggtgcag caaagagatt aaagaaaaca ggaactggaa agttaaagag
     1081 agctaaagct tttaaaaaac atatattaac taaaaaatct gcaaaaacta aaatgaactt
     1141 aagaaaatca actttagtaa gtgatggaga tgctaagaga atagcacaat tattaccata
     1201 ctaagataca gattgtaaat aataactata gaagagggat ataaaaggag gtcataacga
     1261 tggcaagagt taaaaaagct atgaacgctc gtaaaaagca taagaaaata ttaaaacttg
     1321 caaaaggatt cagaggatct agaagcaaat tatatagacc agcaaataca ttcgtaatga
     1381 aagcattaaa aaatgcttat ataggaagaa agttaaagaa gagagacttc agaaaacttt
     1441 ggatacaaag aataaatgca gctgctagaa tgaatggaat atcttattca agattaatga
     1501 atggattaaa attatctggt gttgaagtta acagaaaaat gttatctgaa atggctatac
     1561 aagacccaga aggatttgca aaattagcag aagttgctaa agcaaaatta gcttagtatt
     1621 gaatcatgag atgtaaagtc tttgagttat gtaaataatt caaggacttt tttatttttt
     1681 gaaaaatact tattagtgtc atgtacaacc aattttataa tgataaataa aataagcaat
     1741 acaatagaat tttatgagat ttattataag aattgttaca attatttatg atagctataa
     1801 aaagtcatag taaaaatgat attgatgtat gttgatatat gtataaagct ttcaatcaat
     1861 cttgaattaa tattatcaac atgataatat tatcttatta ataatataaa taagggggag
     1921 ataatatgaa tgaaaaacta atggattgtt ttgtaaaccc aattaaatgc aagttattga
     1981 ttgaaatttt ttcaaataat cgaactacag ctaaacaatt aggagaaaag tttcaagata
     2041 tatcaaaggc aacactttat cgttacctta ataaaatgtt gaaagatgat attataaaag
     2101 tggttgaaga aaataaaata aggggaactg ttgaaaaagt atatgctatg aagttagata
     2161 tagctgatga tgtgaacaaa attatagatg agaactctgg agaagaatat gctcaactgt
     2221 ttactcaata tatgatggga tttttaagag aattttatga atacgctgaa agaaaggaaa
     2281 aggatatcct aaaagatggt tcaggatttt ctgtctgtcc agtatatgct agtattgatg
     2341 agatagttga agtgagtaaa aaaattgcta gtatactaga acctctaaga aataactcac
     2401 atgaagaagg tagaagaatg cattctatag gagtgataat tactccacct aaagaggatg
     2461 aagggaaata aatctgattt acatggggta gagccaattt atacagagaa tcgttttatg
     2521 atttacatat gtatccagag caaagtatct ctgcttggat tgtacttcat tgtaccattt
     2581 catccaatga cttggagtgg tacaatgaag ttttttaatt tttcatatta atgctaaaga
     2641 aatgaacttt agaccacata tgcaattagg ttgcattata caattagacc acatatacaa
     2701 aataaaaatc agtattttta tagcagataa attgactgtt attcttctta aaaatcatct
     2761 attagttatt gttagcatat tgaaagaaat tatagtaatc tattgaataa atgaatattg
     2821 cccataatca tataaactgc tatagtttaa aataaagcag gacgttaaat tttatagtaa
     2881 tctaaaaatt taagaggggg attttatgaa aataaaattt gttattatgc cattggtgat
     2941 atttttaatg gtattatttg tggggtgtca aagtacaaaa gatacagaag ttaaagcaaa
     3001 agatacagaa attaaaatat tagggacagg tgatttacat tcaattttga cagactctat
     3061 ggtttcatat ataaatgagg aaagggaaaa gaataaaaat ctgcttatgg tagatgcggg
     3121 agatttctat ggtacacaaa gtagagaaat gtgggaatgg tcttctggaa aaaagttaat
     3181 taatattaaa agaaatggta gagcagagta tgatgacata attaagagta gtaaagatga
     3241 agtacctata gttaaagata tggcaaagtt gaaatatgat gctgtagttt tgggagataa
     3301 tgaatttatt tcaaatgata agcaaagtct tgataaactg gtttctgatt ttaaaaataa
     3361 taatatgcca ttggtatcag caaatatata tgaacaatct ggagaaaatt atgtacaacc
     3421 atatgtaatg aaaaatatta agacagatga gggcaatgta aaagttggaa tactaggtct
     3481 tactataaaa gaagtagggg aaacccttgg tcttgaggat tttgaaaagg ataaaaaagc
     3541 tagagagctt ggagaacaag ctgattacaa aggaaaatta tatgcaaatg atttagtaga
     3601 agatgcaaag aaatggataa aagttatgga aaaagaaagt ccagacataa tagttgcagt
     3661 tgtacactca ggggaagaac ctaaaacaga tagaaatcca ggaaatagaa taaaagagtt
     3721 agcaactaca gtggatggaa tagatgcaat tgtagctggt catactcatg aaaaaataga
     3781 tgaacacaaa tataaaaata aatctggaga tgaagtaatt gttacacaac caggttggca
     3841 tggagataga gtatcagaga ttaatttcaa attaaataaa gagaatggaa agtgggacat
     3901 aaaggaaaag tctagtaaat taaaaataat tgataaagaa gtaagtatag ttgctactgc
     3961 agatttacat tcacatttct ctgatgaatt aacagttgac cttgttaatg aacgtagtaa
     4021 cccaattgaa cctgttgtag tagatgcagg cgacttctta gaccctcaaa cagatgaaat
     4081 gactcaatgg tataaagagt ggaaaagtat aagagataat aatagtgata aaacgattag
     4141 tagatgccca atggtcatgg atatgaatca gggaatgtat gatgcagtag tacttggaaa
     4201 tcatgaattt gtatctgaaa aagatgaatt ttggtcagat gaacgccttt tagatgctgt
     4261 tatagaggat tttgaacagg tggcaatttc tgtgttatcg gcaaatatat ataggcaatc
     4321 tggtaaaaat tatgttaaac catatattat aaaagatgtg gagacgagtg aaggtaatgt
     4381 aaaagtagga atattgggac ttactataaa agaagtaggt aaaggtctta aaaatgtaaa
     4441 cttacaagag caatttcaat ataaggataa attgtatgca aatgatttag tggaagatgc
     4501 aaagaaatgg gtaaaagtta tgaaaaagaa aaaaccagat ataatagttg cagttgtaca
     4561 ttcaggagaa gaaccaaaaa atccaaaaca tccaggaaat agaataaaag aactagcaac
     4621 tacagtagat ggtattgatg caattgttgc tagtcatact catgaaaaaa tagatgagca
     4681 tgaatataaa aataagtctg gagaaaaagt tatagtgaca caaccaggtg agcatggtaa
     4741 atattattct aaaattacct ttaaagtaaa taaagaaaag ggttcgtgga ctattaatga
     4801 taaattcagt aaaacaattg aggttgaata actttataaa atttatagat aaagtaaatt
     4861 atctgatatt gacatgatat ataagataaa gtttctaata ttggtataat gcataatgtg
     4921 aaatatttta ataataagag aataaacaag acaaataaaa atattttaac atgtaagata
     4981 tagtatgaac tattatacta atgcttactt tataatttga acatgttaga ataagtctta
     5041 tttgaaaatt ttagtgtatt agagtaagta ttattttact agtaaaaaat attgagaact
     5101 atatgtagat ttgcttaaaa aggaatttac atatagttct tttcgtataa taaatagtac
     5161 aatcttatat ggtaaagata aagtgtgata ttaaatttta ttatcatcca aaatctaagg
     5221 aggagtctct tatgaaaata aaatttatta tagtagcatc aataatattt ttaatagtat
     5281 tacttgtggg gtgtcaaagc actaaagata cagaaatcaa tgtactcgcc acaagtgatt
     5341 tacattcaat tattccagat aatttaattt cctatgtaga agaggagaga aaatatgatg
     5401 agaatctact tatggtagat gcaggagatt tttttgacat gcaaagtggt gagatgaata
     5461 agtggtttac tggtcaaaaa ttagtaagat ttaaagatgg gataccagag tacaaaaaaa
     5521 tagctgaacc acgtgaagga gaagttccat tggctaaaaa aatggcaaag ctgaagtatg
     5581 atgcagttac atttgggaat catgaatttg tatctaatga caagcagagc cttgataaat
     5641 tggtttctga ttttaaaaat aacaatatac cattagtatc agcaaacgta tatgaacaat
     5701 ctggagaaaa ctacgtaaag ccatatgtaa tgaaaaatat aaatactgac aagggaactg
     5761 taaaggttgg tatacttggt ctgactataa aagaagtagg ggaacgttca aggtttaaaa
     5821 gttttgaaca agataaaaag gctagagaac ttggggaaca acctcaatac aaagggaaat
     5881 tatatgcaaa tgatttagta gaagatgcaa ataagtgggt aaatgttatg gaaaaaaaag
     5941 aaaagccaga tataatagtt gccattgtac attcaggtga agagccaaaa aatcctaaaa
     6001 atccaggaaa tagaataaaa gaactagcaa caactgtaga gggaatagat gctattgtag
     6061 caggtcatac acatgaaaaa atagaacagc atacatataa aaataattct ggtgaagaag
     6121 taatagtaac acagcctgga gctcatggta atagtatatc aaagattaat tttaagttgg
     6181 ataagaagaa tgataagtgg tttataaaag acaaatatag tgaattaaag gtttttggaa
     6241 aggaagtaaa tataattaca acttctggtc tgaatccaaa attttcagat gaaatggcag
     6301 ttaatttgtt taatgaaaga gatagagatg ttcaacctat ctttatagat actggagatt
     6361 ttttaaatac taatacaaaa gaaatgacta agtgggctaa agagtggcaa tacaatgaaa
     6421 ataaaggcat ctataaaaaa ataaatgaat ggccaattgt atatcttggt tatgatgctg
     6481 tagtacttgg tagccatgaa tttacagagg ataaagagac ttttgaggaa aaaaaatcta
     6541 cattggatta tattatagaa aactttgaag aaatgaagat accagtatta tcagctaata
     6601 tatatgatga atctggagaa aattatgtaa aaccttatat aattcataat gtagaaacta
     6661 gggaaggaaa tataaaggtt ggagtattag gtcttactac tactgataag gatgaagaag
     6721 acttaagtaa ctcaagctta agcaaagact caaagacttc gaaaattcct gaagaaacag
     6781 gaaagttata tactaataat ctagtaaaag atgcaagcga atgggtaaaa gttatgcaaa
     6841 aggaaagtcc tgatgtgatt gttgcagttg tacattcaga taataatagt actctaaaaa
     6901 atccaagtag tgaagtcaaa aaacttgcaa tgtcagtaga tggaatagat gctattgttg
     6961 taggtcatac gaaaaataag gtagaagaac atatttacaa aaataagtct ggggatgagg
     7021 ttatagtaac acaatctgga gaagattgtt attcaaaaat aagtcttgaa ctaagaaaag
     7081 aaaatggcaa gtggaatatc ataaacaaat atagtaaagc aataaaaatg aagtgaattt
     7141 attgatatat aaaccattat taacactgat ttttataata taataaatac tgat
//
LOCUS       DAKBNN010000192         7100 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000192 DAKBNN010000000
VERSION     DAKBNN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.195"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            77..1858
                     /locus_tag="OUB02_003220"
     CDS             77..1858
                     /locus_tag="OUB02_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCT8886949.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1940..2470)
                     /gene="lepB"
                     /locus_tag="OUB02_003221"
     CDS             complement(1940..2470)
                     /gene="lepB"
                     /locus_tag="OUB02_003221"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCT8886950.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDENEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2510..3334)
                     /locus_tag="OUB02_003222"
     CDS             complement(2510..3334)
                     /locus_tag="OUB02_003222"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCT8886951.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3591..4499
                     /locus_tag="OUB02_003223"
     CDS             3591..4499
                     /locus_tag="OUB02_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8886952.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4643..6088
                     /locus_tag="OUB02_003224"
     CDS             4643..6088
                     /locus_tag="OUB02_003224"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCT8886953.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWTQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIINDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6400..6852
                     /locus_tag="OUB02_003225"
     CDS             6400..6852
                     /locus_tag="OUB02_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8886954.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2927 a          844 c         1058 g         2271 t
ORIGIN      
        1 atttgcctat aaaaagtata tataaaaata tagttcaaaa tggtataatt aagtataaca
       61 gaaggaggta atgtaagtga gccaatacat aaatataaat tctcagttag acgccctaaa
      121 attgaaggag ttcgagaaaa tattttacga aaatatcaat gaggacgtta aagtagtgcc
      181 aaaagttact ccttttaaag gaataaatac cgatttgttg tatgttaaag atggaagaat
      241 tttatttata aaatttatgg atacaacaga agatatattt tttattttag aagaagagtt
      301 acttgaagtt atgaatgagg aatatgaact tttgaaacta aaaatgggac aaaagaatag
      361 aaatataagt tataactacg tttacattat gccatacgtt gaagttgagg aaacatatga
      421 atttgaagaa tttgttaata ataatataat agataaaaat aagcttcaag atataatgaa
      481 taagggttcg ctggaggaat atcttaatga tgagaatgat gaaataaact taaatttatt
      541 tttactagat atatgttcag aatactatat aataaatgat aaacttcatc taaatgaaaa
      601 atttaaaaaa atttcattct acaatgatga ctataagtat acagcaacta tgatggaaga
      661 cgttcaaatt aaagatgttg tttctattaa atatgggaat acacttattg aaggtggttc
      721 tggtataggc aaaacagcaa ttatgctttc tagggctata aaattagcaa aagtatatcc
      781 acatcataaa ttagtcatat ttacacatac aaaacaattg tgcaatgagc ttcgagaaag
      841 gatagagtta ttatacaaag ataataacaa tttagaagtt cacacattta gttcttttat
      901 atttaaacta gctaaaaaat tcaatttaat tgtagattat aatatgttaa aaaatgatta
      961 cgaaaagacc tttaataatc ttgtaaaaca agcacagaac attataaaaa ataaaaatat
     1021 gttcaaggct atttttattg atgaagcaga aagcttttta gagtatgaaa tagattttat
     1081 aagggagttt ttatataaaa caaaattcat atttaatgta tgttcttgta actccttgaa
     1141 tatctctaat agattgaata tatttaaaaa attatataat ggtatagaat ttgatgataa
     1201 gattatctta agtaaaaatt atagacaagc taaagagatt gtagatttta caaataaatt
     1261 ttcaaataat tctaactcat atataaacga attaagacca aatacagaat ttagtacttt
     1321 cttttatact aaagcactta gaggtggaaa taagtctgtt gatataataa aagtaagtga
     1381 ccttgatgac caaataagtt ctgtaatctg ggaaattgag tatttaataa gtaaaaaagg
     1441 tcttgattat tctgaagttg ctatcgttta cccttacaat aagaagaaat taaaaagtgg
     1501 aaaaaccatt tactttcaat atatgttaaa aaaagcattg gaagaggcaa aaattcctta
     1561 tatatgtgca gaagataatt tgacaaatat aagtaagaag gtaggaacta caatagcaaa
     1621 tatatatgca ataaagaatt tagagtataa agcggtaata gtatgtgaat tagagatgtt
     1681 atataatcaa actataaatg atattgaaca agattatcaa gtaaatgact ttgtaggaga
     1741 tttaaataag gtttatttag ctatgagtag agcaacagac tacctaagta tagtaacaac
     1801 ttttaatgaa gaagcaagtg atataataag acttattaca gaatcaaaag atatttagta
     1861 aaaaaatatt tgtaatttaa gaaaataaaa agagactgag atttgtaaaa taaactttac
     1921 caattcagtc tcttattttt tagtctactt tcccaatatt atcaagaggt aatagtctca
     1981 ccataacctt accaagaact tcattttcat caaccatacc tacatcaggg aatctactat
     2041 cattactatt ttctctatta tctcccattg caaatagttt accttttgga acaacagtat
     2101 ctatatctcc agaagtatag ttattgtgta tatatggttc atttaataat tttccattta
     2161 catacacttt agaatttgat atttttattc tgtcaccttc agtagctata actcttttta
     2221 ttaagtcttt tttctttcct gtttctccat caactaaatt ggttttaaaa actacaatat
     2281 cgcctttttc aggtttacca accttgtatg atatcctatt taaaatcaga taatctttat
     2341 catctaaagt aggatacatt gattctccac ttacaataga aggtcttata aattgtagaa
     2401 ttacaaatgc aagtacaata gccatagcta ttgacttaat ccaatcaaat atttcttttt
     2461 taacactcat aacttttctc cttatcattt gttattaaat tagaatacat tataatagaa
     2521 agaaataagc aagaaccact atacccaaga ctattctata ataaccaaat actttgaagt
     2581 catgtttttt gatatagtcc ataaaaaatt taataactac aattgaaaca acaaatgcta
     2641 ctacagaacc aacacctaaa ataagccatt caaatccagt aaatccaaat ccagctttta
     2701 gaagttttaa tgcactagct ccaagcattg tagggatggc taagaagaat gaaaactctg
     2761 ctgctacata tctagaagta ccaagtaata ctgcacctat tatagtagaa ccagacctag
     2821 atgtacctgg tataagagct agacactgga ataatcctat acaaagagct aatttatatg
     2881 taacttgaga aaaatcttta tacttaggtt ttttatttct cttttcaagc cctatcatta
     2941 ttataccata tacaataaga gcaatagcaa ctactgttga attaaagaat aatctatcta
     3001 tatcatcttc aaacagtatt ccaataactc cagatggtat aactgcaatt ataaccttaa
     3061 gccataaacg gactgtttct tttttctgct taatattctt tgcgctatca aaaggattta
     3121 attttctaaa aaatataaca agtacagcta atattgaacc aaattgaatt acaactaaaa
     3181 atgtacttat aaatgtattt gaaaaattca attttataaa ctcatcaact aatatcatat
     3241 gccctgtact acttactggt agccattctg ttattccctg aactatgccc aaaatgactg
     3301 ccttaaaaat ttcaagcaaa ctaaatgttt ccaaatcaaa atacctccaa tacaaattta
     3361 tagtagcata ttttattcat agattaataa aaaaatgcca ccttttacta acttataata
     3421 ccatagtact gtgtagtgca caatggaaga ttgatatttt cataaaacct tcataatata
     3481 acaaaataaa cttaaaagtt atctattgaa taaggggtat atttgagggg gaaatatatg
     3541 gtatagtaaa agtatagcta ttttatttta tgaaaaggaa aggagttatt atgtatagta
     3601 caaaaatact agatgattat gagtatgaaa cttttaatgt tttttctaca aaattaaatc
     3661 caaacacgaa acacgattat ttatcaaagg ttatactgtt taaagaattt ttaaaaggga
     3721 aagaattgat ttatgctacg aaagaagatt gtaaaaattt tgtagactat atacagacta
     3781 aatatgcgaa atcaacttgt gaaaagatat atagttattt acatagtttt tataacttct
     3841 taaaaaaaga aggatacata gatataaatc catttagata tgtagaaaaa cccacagtaa
     3901 caagaataaa aacaaaagat gatgttctaa gtatacaaga aataaataaa cttataggta
     3961 tcctaccaaa attaaatata agagatagag taataatagt ctgtctagtt actactgggt
     4021 gtttattaaa tgaattagtt agcttaaagt ggaaagacct tatggtagat gaaaacgata
     4081 actcatatgt aaggttaggt aagggaagaa aagaaagagt agttaaatta catccatact
     4141 tttttaaact tttagaagat tacaggaatt attctgggct tccagaggta ataataccta
     4201 gtgatgattt tatttttaca actcaaaaga gtaattctat aacagataga aatgtcagac
     4261 ttatagtcaa aaaagcatta gatttagctg gactttctca atattcagca agagatttta
     4321 gacattcatt tgcagcaata agtttaaggc ttggggcaga tgaaagtgat gtaaaaaatc
     4381 agcttggatg gagtgataaa tactatgcaa ttagatataa gtatgtatta aattttgttg
     4441 atagtgaaat agtggattac ttaattgaaa aagataattt ggatataaat aaaaaatagt
     4501 aataaataaa aaatactata tataaatgtg aacaaaaaat aaaaaagtct tgttatagtt
     4561 cgtaaaatta tatataatta tttatgtact aatgtgtaca cagtaatcac acgacaaaag
     4621 atttaaggaa ggtgctaata taatgataga taataaatat actacttact caaatccact
     4681 tacagacaga tattgtagca aggacatgag ttatatattt tctccacaat ttaaattctc
     4741 tacatggaga aggttatggg ttgcacttgc agaaggtgaa catgagttag gaatcaatat
     4801 aactgaagaa caattagatg agctaagagc taatatagat aatataaatt ttgatgaagc
     4861 tagaaaaata gaaaaagaag taagacatga tgtaatgtct cacgtaaaag cttatggttt
     4921 acaatgtgaa aaagccaaag gaataataca tttaggagca acatctgcat atgttggaga
     4981 taatacagat gttatacaaa tgaatgaagc tttaaaactt ctaagagtaa agcttgtaaa
     5041 tttaataaac aatttaaaag aatttgcact taagtacaaa gatatgccag ctcttggatt
     5101 tacacacttc caagctgctc aacttacaac agtaggaaaa agagcttgtt tatggactca
     5161 agatttaatt cttgatttgg aagatttaaa ctatagaata gacaacatga agttaagagg
     5221 agttaaaggt actactggta ctcaagcaag ttttttaagc ttatttgaag gagaccacga
     5281 aaaagtaaaa gagttagata agaaaatttg taaaaagatg ggataccatt cttcctatgc
     5341 agtaagtgga cagacatata ctagaaagtt agattatcaa gtaataagca tactatcagg
     5401 gatagctcaa agtatgcata agatgactaa tgatataaga cttttacaaa gtttaaaaga
     5461 attagaagag ccatttgaaa aaaatcaaat aggttcatca gcaatggctt ataagaggaa
     5521 tccaatgaga agtgagagaa tagcatcttt atctaagtat ataatctctg aatctataag
     5581 ccctgcaatg gtacaagcca ctcaatggtt agagagaaca ttagatgact cagccaataa
     5641 gagattggca atacctcaag catttatggc agcagatgct atacttgaaa tagggataaa
     5701 tgtaactgat ggtttagtag tatatgaaaa tatgataaat agacgtgtaa atgaagaact
     5761 tccttttatg gctacagaaa caatacttat ggaagctgta aaaagaggtg gagatagaca
     5821 ggaattacat gaaataataa gagaatattc tatgaaagct gcctatagag taaagcatga
     5881 aggcaaggat aataacttaa ttgaacttat aataaatgat gattcattta aaatgagcaa
     5941 ggaagaaata ctatctataa tggacccaaa aaactttata ggtagagctc cagaacaagt
     6001 tgtagaattt gtaaatgaag ttgtagaacc tgctataaaa gattataagg aagacttagg
     6061 aaaagtagat gtagacctaa gagtatagaa ttgtacagat aaaatataat taaaaacggc
     6121 tatcttattt ataaataaga tagccatttt aatgggctag cgtcttctct ccaccgctgt
     6181 gaagagttct tctttactta ctataccatg aatttaataa aatagctata taaataatat
     6241 ttaattagga ataatataaa taaaaataaa aaaatgataa gattactaat taaatttaaa
     6301 cattcagcaa aaactattgt acttgcaata tttttaatgt aaactgaaat aaagttattt
     6361 agataattta ataaatttta aaacaaccga ggtgattata tgaactatat agtaaaagat
     6421 accataagtt ttttagtaaa aacatttcct aagatttatt ctagcctgta cttagaagac
     6481 ctaaaaaaat ttgcaccaga ttataatgta aataaaacac aattaagagc attagttttc
     6541 ataaaaaatt atggagtaat aagtatgact gatttatgct ctaagttaaa tatagaaaaa
     6601 ggcagtttaa ctagtatggt tgatgattta actgataaga agtatgtaac aaggaaaaga
     6661 gatttagttg atagaagaaa atatctaatt gacattactg aagagggaga taagatagca
     6721 actgatttta tggataaatt aagtgatggt ttaggagaaa aactttctaa attaactgaa
     6781 gaagacagaa aaaaatattt agaagcaata aatactcttc aatatatatt aaacaaagaa
     6841 gaatttagat agaaaaaata taaaaatttt tgtatttaat ggtactaaga ttaattcttt
     6901 gttattcatt taactatata attataagtc taaatacaat ctaaaaagta catcttaaat
     6961 tttcttgatg tactttttta taatttctct aactaaggca agacaagttt tagatgcttt
     7021 aattaaagtg aataaagatt actggccaac actatataaa gaaaatgaaa aatagtcagg
     7081 tctaagtctt aattaatata
//
LOCUS       DAKBNN010000193         7095 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000193 DAKBNN010000000
VERSION     DAKBNN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.196"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            40..420
                     /locus_tag="OUB02_003226"
     CDS             40..420
                     /locus_tag="OUB02_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT8886955.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            545..745
                     /locus_tag="OUB02_003227"
     CDS             545..745
                     /locus_tag="OUB02_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886956.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            941..1669
                     /locus_tag="OUB02_003228"
     CDS             941..1669
                     /locus_tag="OUB02_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8886957.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1691..2608
                     /locus_tag="OUB02_003229"
     CDS             1691..2608
                     /locus_tag="OUB02_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HCT8886958.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2750..3934
                     /locus_tag="OUB02_003230"
     CDS             2750..3934
                     /locus_tag="OUB02_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCT8886959.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            4041..4229
                     /locus_tag="OUB02_003231"
     CDS             4041..4229
                     /locus_tag="OUB02_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCT8886960.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            4378..5028
                     /locus_tag="OUB02_003232"
     CDS             4378..5028
                     /locus_tag="OUB02_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCT8886961.1"
                     /translation="MKKNYEIILFDLDGTLTDPKEGITKSIQYSLNSFGIEEDLDNLE
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            5131..6069
                     /locus_tag="OUB02_003233"
     CDS             5131..6069
                     /locus_tag="OUB02_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCT8886962.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            6095..6946
                     /locus_tag="OUB02_003234"
     CDS             6095..6946
                     /locus_tag="OUB02_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCT8886963.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         2927 a          665 c         1278 g         2225 t
ORIGIN      
        1 atattaggta tttatttaat aataaaggtg ggtattgtta tgataaataa aataggaaaa
       61 ataactctct atgtaaataa tcaagaggat gcaaagttat tttggacaga aaaattgaac
      121 tttgtagtaa cttatgaaaa ggaaatgggt ccaaatatga aatgggtgga agtagcacca
      181 agtaaaaatg aatttacaac atttgtgttg tatgataaaa atctaatgat gaagcaaaac
      241 aaagagataa atgtatctca tccatcaata attttaagta caagagatat taatggaact
      301 tatgaacaaa tggcaaataa cgaggttgag gttggggaat taatggagat gccatatgga
      361 aggatgtttt cttttaaaga ccaagatgga aatgattatt tggttagaga ggataaataa
      421 ttttacaaag tattaaattt actcaatatc tgatataatt agagacaata attattataa
      481 actagtctag gattagaatt atataattaa aaattaagtg taaattttga gtagaggtga
      541 taaaatgttt aagaaaatgg cagtactaaa agatatagca actaaaatag gtcgtaaaaa
      601 agcgtatgaa ctattagaaa tggttgaagg taatgatgcc tttgtagctg aggtaaagat
      661 aaaaaagaat ggaatagaat ctaaaaaaga agaaattatg ttaaaagata atcaaaaaat
      721 aatattagag tatatagaag gttaaatttt aaatacatat aagttgatat actaaatttc
      781 tgagatgagt ttacataaaa ctcatctttt ttacttttca ataactttgt tttattattt
      841 aatatacatt ttatctcctt agagttgctg atatataaaa attactgata taataaaaga
      901 atggatatta atgtaatatt ttatagaaaa gaggggtatt atgcttgaaa taaaaaatct
      961 ttcctttaat gttgagtcta acaatgagga actaggaata ataaatgatg ttagcctttc
     1021 atttgagagg ggaaaactta ttgttatcac aggtccaaat ggtggaggaa aatctacaat
     1081 tgctaagctt attatgggaa ttgaaaaagc tacgagtggt cagataattt tagatggaga
     1141 agatataaca aatcttagta ttacagaaag agcaaaaaaa ggaataggat acgcatttca
     1201 acaaccacca agaataaaag gtatgacagt tgaaaattta cttacacttg ctcatggaaa
     1261 accattgtct actgatgtat gttgccaata ccttacagat gttggattgt gttcaaaaga
     1321 ctatttaaat cgtgaagttg ataactctct ttctggaggg gaaatgaaaa gaattgagat
     1381 agcaactctt tttgctagag atttaaaggt ttctatattt gatgaacctg aagcagggat
     1441 agacctttgg agctttggaa aactcaatga aagttttaag aaaatacatg aagagtctaa
     1501 tcagactata atcataattt ctcaccaaga aagaatatta gagttagctg atgaaataat
     1561 tgttctacaa gatggttcag taaaaagcca tggaacaaaa gaggctatat tacctgaaat
     1621 catgtgtcaa gttaattcaa gttgtgaatt aatgaaagac atgaattaat tagaaaggaa
     1681 ggcaaattaa atgaacaaaa tagatttaaa tcttttagaa aaagttgctg aacttcgtgg
     1741 aactccatta ggagcataca atatacgtaa aaatgggcaa ggagtagaga gacattcaac
     1801 tgaaaatgta actataattc ctaaaactga taaacctgga atagatatta tagtaaagcc
     1861 aaatacaaaa ggagaaaatg tacatatacc agtaatagta acagaggctg gtgtacagga
     1921 tatggtatat aatgattttg aaattggaga aaatgcagat gttgttataa ttgctgggtg
     1981 tggtatacat aactgtggtt gtgatgatag tgaacataat ggtatacata gatttttctt
     2041 acgtaaagga gctcgcctaa aatatgtaga aaaacattat ggtgaaggtg atggagaagg
     2101 aaagagaatt ttaaatccag ttactattgt caatatggat gaggattcat attgtgaaat
     2161 ggaaacaatt caaattaagg gtgtagattc aactaaaaga gaaatggatg caaacctagg
     2221 taaaggagct aaaatagttg tatttgagaa attattaact catggtaatc aagttgcaga
     2281 atctaatatg aaggttatct tagagggtga aaactcttct gctcaagtag tttctcgtac
     2341 agttgggcaa gataattcta agcaaaagtt taatccttgt gttattgggg attccctttg
     2401 ttctgcacat gttcaatgtg attcaataat tatgggaaat tctcaaatta gtgctatacc
     2461 tgaaattgct gcaaatcatc cagatgctct tttagtacat gaagcagcta ttggaaagat
     2521 tgcaggagac caaatactaa agatgatgac attaggtctt actgaagaag aagccgagca
     2581 acaaatactt aattgcttct taaagtaaat taaagatttt atttaattta atataattaa
     2641 tatagtataa tatagtgaat aagtctaaaa tatttttatt tttaggctta ttttctttat
     2701 gtaagaaaag ctattagaga aattatattg taattaggat gtggaataaa tgaaaaattt
     2761 acttataaga aatgggacaa taatatctcc aacagatggt atcaaatttg aaaaaagaga
     2821 tatcttggtt ttacatggga aaatatatgc tattggattg aaccttaaag agaaaataag
     2881 aaaagaaaaa ttacttacaa ataaagagtt cgagataata aatgccgata atatgtatat
     2941 ttccccagga tttatagatg ttcacaccca ttgttataag agaaaattac ctacaggtat
     3001 ggattctgat tcaattggaa ttgaaaaagg ttctacagtt atatttgatg caggtacagc
     3061 tggtccctca aactactatg attttaaaaa aaagtatatg gatgagtgta aaacagaagt
     3121 atattctttt ttaaatgtta caaatgaggg actaaatata ttgaatgaat caacttcttt
     3181 agatgttata aattttgaca aggttgagtc aattgtagaa aaaaatatag aaaagataaa
     3241 gggtataaaa gtaaaagcga gtaaatttca atctaatgaa agtggtatag atataattaa
     3301 gaaatcaaag caagttgcta aaaaattaaa tttaccctta gtggtatata taggagagta
     3361 tccatcatat atagatgaag taatgaatat tttgggaaaa ggagatgtgg ttacaccggt
     3421 atatggaaat agtacaaatg gaatccttaa agaatctggc acattaagaa aatcagtaat
     3481 aaatgcaaag aatagaggag tattgtttga tgtatgtcat ggagtggacc aatttgattt
     3541 taaagttttt aaaaaagcaa taaagcaagg tttagagcct gacctaataa gtactgatat
     3601 gcatataaaa aacatgaaag acgtaaacta ttctctttta aatgttataa ataaaattat
     3661 ggaacttggg atatccctag aaaagtgtat agaaaaagta actgaatacc caacaaagat
     3721 tttcgattta catggaaata aaggcagaat aaagattggt ggagaaggtg attttacaat
     3781 atttacaatg gaggagtgta atgatattat aaaagactac aataataatg aacttgtttt
     3841 aaataaaaaa ttaagattgc aatatactgt aaaatcatgg atgaaaagca gtgaagttta
     3901 tagacatgga tacgaaaata ctataataaa ctaaacaata gtgataaaat aataaatata
     3961 tgtttaaatt ggtatatatt ggataaatat ctaaggaaac aacaattaaa aaataataat
     4021 tttaggggag gtattttagt atgaaagcat ttgttgataa ggatgtatgt gtagcttgtg
     4081 gagcatgtat aggaacatgt ccagacgttt ttagtatgag cgatgaaggg cattcagtag
     4141 caattgaagg agaaatacca gcagatttac aagatttatg taaagaagcc gaagaaggat
     4201 gtccagtttc agctataaca gttaaataga gctgtttaaa tttattttta aaataaatat
     4261 ataagattta attggtactt aagctatagt attagaaact tctattacta tagcttttta
     4321 aaatagataa acttaatata taagatatta ctttttataa atatggagga ctgaaatatg
     4381 aagaaaaatt atgaaataat attgtttgat ttagatggaa cacttacaga ccctaaagaa
     4441 ggaataacaa agtctatcca gtattcttta aatagctttg gcatagaaga agatttggac
     4501 aacttagaac aatttatagg accgccatta catgatactt ttaaggaata ttataaattt
     4561 gaggataaaa aagctaaaga agctgttgaa aagtatagag aatattttgc tgataaaggg
     4621 atatttgaaa ataaaattta tgagaatatg aaagagatat tagaaatgct ctataaaaat
     4681 ggtaaaatat tattagtagc aacttctaag ccaacagttt ttgcagaaac tatacttaga
     4741 tattttgata tagatagata ttttaaatat atagcaggta gcaatcttga tggtacaaga
     4801 gtgaataaaa atgaagttat acaatatgta ctagacctat gtaatgtaaa agataaagat
     4861 aaagttatta tggttggaga tagaaagtat gacataattg gagctaaaaa gataggtata
     4921 gattctattg gagtattgta tgggtatggt tcttttgaag agattagtga aagtgaacct
     4981 acttatattg tcgaaaatgt agagtctata aaagatattt tgttataaat atcttttata
     5041 gacaaaaata ttacaaaatg gtgtcgcata tttaaaaata tgatattata gttaaggaag
     5101 tcatttaaaa aagaggagga tgcacttaaa atgaaatcta ttttaattat gggtggaagc
     5161 gacttcatag gttctgcact tgctaagcgc ttgattaaat gtgggtatca aattgatata
     5221 cttacaaatg gtaaaaagga aatagattat aatggtttta aagaacatct aatttgtgat
     5281 agaaaagttc gaaaagatat ggaaaacatt atcacaggaa gaaaatatga ttacatatat
     5341 gatatgacag catatacaaa agaagatgta agtaatctga ttgattttat aagcatggat
     5401 aacttaaaaa agtatatagt attgtcagca ggagcagtat ataaagatag tggaagaaat
     5461 ataaaagaag aaaatgagaa aggtgaaaat gaaaactggg gaaaatatgg gttaaataaa
     5521 aaagaggctg aagattttat tataaatagc cctataccat atataattat tagaccaact
     5581 tacatatatg gtgaaaataa taatttatat agagagtatt atttctttga aaaaatagaa
     5641 aaaaatgaaa agataccagt accaaaagga aaacaagtta gtaatcaatt tatatatatt
     5701 ggggatttgg taaaggtatt ggaaagcata atgaaaaatc ctcatgtaag agaggcctat
     5761 aatgtgacaa atccacagtt aatttcttgg gatgacctta tatatacatg tggagaaatt
     5821 attggcaaag aacctataat aaaatatgta gatatggaaa aagtagagtt tagagaaaga
     5881 acgtatttcc catttagaaa tatagacttt aatcttgata taaataagct tattgaacat
     5941 gggctttata taccaaacgt gttactaaaa gaaggtctta cagcaacata taaatggttt
     6001 tcagcaaata agccaaaaat gcatgacaga aaaatgaaca aagttgagtc tgtaatgcaa
     6061 atagtttaaa aaaataaata agtaggtaga tattttgaac tcaaaaatta agtatttttt
     6121 aaaatttttt atcttaatat ttattttgat ttttatatac tcagttgcga tagagccgag
     6181 tctattaatt gtgaaaaaat ataatataag tgaaaatgga gcattgataa aaaatgatac
     6241 aattgagagt aaatctgaaa gtgtaaaagt agttcaaatt tcagatactc aaataggtag
     6301 tttttattca actaagaatc tcaaaaaagt agctaataag attaacactt tgaatccaga
     6361 tataatagta tttactggtg atttaattga ttactccaat aaaaatccaa gtgtagatga
     6421 aattacaact attttatcaa gtatgaatgc taggttggga aaattttcag tatttggcaa
     6481 tcatgattat atgtataaac ttcctaggta ttataggcag attatgaaaa attccaactt
     6541 caatctttta gtaaatgaaa ataaaaaaat aaaattaaaa gatgataaat atattaatat
     6601 attaggtgtt gatgaaatat taaatggaaa tcctaatata aaatatctgg agagtcaaat
     6661 agataataaa agttttaatt tattgttagc acatgaacca gatttagtag atatgcttag
     6721 taaagataca atgaatttag ttctatctgg gcatagtcat ggtggtcaaa taagattacc
     6781 tattaaggga gcattagtta ctcctccata tggaaggaaa tatactaaag ggttttatga
     6841 tataaatgga aatcatttat atgtgagttc gggtcttgga agtacaaagt taccatttag
     6901 attttttaat ataccagaga taatagaatt taatattata atctaacata aaatatttag
     6961 aaaatataat tttgcttttt ataatatgta gatgtatata ctgtaaaaat ggattaaacc
     7021 agtagattga atgaattaat agattaaatt aatatataaa atatttaagt tataattaat
     7081 gtattagaca aaata
//
LOCUS       DAKBNN010000194         7092 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000194 DAKBNN010000000
VERSION     DAKBNN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.197"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..541)
                     /locus_tag="OUB02_003235"
     CDS             complement(<1..541)
                     /locus_tag="OUB02_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8886964.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSK"
     gene            1012..1662
                     /locus_tag="OUB02_003236"
     CDS             1012..1662
                     /locus_tag="OUB02_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HCT8886965.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(1802..3178)
                     /locus_tag="OUB02_003237"
     CDS             complement(1802..3178)
                     /locus_tag="OUB02_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8886966.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(3194..5107)
                     /locus_tag="OUB02_003238"
     CDS             complement(3194..5107)
                     /locus_tag="OUB02_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCT8886967.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            5243..5875
                     /locus_tag="OUB02_003239"
     CDS             5243..5875
                     /locus_tag="OUB02_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCT8886968.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            6014..6553
                     /locus_tag="OUB02_003240"
     CDS             6014..6553
                     /locus_tag="OUB02_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HCT8886969.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(6729..6899)
                     /locus_tag="OUB02_003241"
     CDS             complement(6729..6899)
                     /locus_tag="OUB02_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8886970.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2623 a          969 c          938 g         2562 t
ORIGIN      
        1 cctttgaatc aatttgtaat aaatttatta tttctataaa tgtagaaata tctatatatc
       61 tgtgacctaa atcaaatata tctgagataa agctaggtac tttttctgga actcctggag
      121 ttatagctat ttttatattt ttatagttat tatcattcat atatggaatt ggtctaatat
      181 tagtcactcc aattttataa aataaactta tagtttctag agtagtttca tcagaatcat
      241 ttacgattag aacatttgta ccctgtggta aagatagtag attataaatt ttatcttccc
      301 taaaagttct tcttaccaca atcacttttt tcttatctag tatattattt ataattttgt
      361 ctaatctttc tactgacatt actaatatca catcatcatt tataatatca ttatcattta
      421 tttcatttat gaagtatcta ttaatattta tagaatctcc aaaaactaat ttcaaattat
      481 tttctaaaaa gtctattaaa tcttttctac tatcatttac cagagctact gattttttca
      541 tttatttctc cttatataat aaatagtttc caacaaaata gttgtataaa gcaatataat
      601 tttgataatt taaatttatc aaataaaaaa gaaagtgtct ataatgactt tataaaagtt
      661 gcattaagac actttctttc ttataattaa aaatcttgtc accaaatccc cctaattgtt
      721 gcttaaaact taaactgtat gtatattttt catttctatt cgattaaatt ataatataca
      781 cccaaaaaaa tttattttaa taaattctat tgataatata attttgtaaa tatagaatta
      841 tattaaaata aattatatat gaaaatatat atgtataagt ttattctgcg tgagtagaat
      901 gaatttgtca agtttaattt agttgtaatt gtatctttta ttaaaaataa tataaattaa
      961 aacatataaa taaaatattt tatacaatat attgtaaagg aggtaacttt gatgaaagca
     1021 gaaaaaacaa gtatatatcc aattattaaa aataatttaa acaaaatatc aattgttgaa
     1081 tataaaaagg gtcaaagatt tgtagcatca gataaagact tacaagaagt gttctttata
     1141 atagagggag ttgcattagt ggagtgtact actagaggag gaaataaatt tcttgttgac
     1201 attgtacctg aaaatgaatt tataggcaaa atcagttaca tatatgaaca taatttgaaa
     1261 tgtgatatat ttgcaaagac taatataaaa ttatttcgat ttgaaaaaaa tgtatttgaa
     1321 gagttttatc tcaaaccaga ttttattgca ctatttcata gaaaatgtac aagaagaata
     1381 tatgaattat ataaaaccag aatggtaaga gaactatttt cttgcacaga agttattgcc
     1441 tattgtatat caaataaaca agaaaataat gtctgcaata taaaagctat tcgtaatttc
     1501 agtgaaatct attcaattag tagaaaaagt agataccatt ctctgctaaa gttaactgaa
     1561 aaggagatag taaaaaaaat gggtaataca tatgaaattt taaactatga tgaattacat
     1621 aaattggcat ttgaagtaaa agaatttttg gaagatgagt aaaaattgtt acttaatcaa
     1681 aataaagtat aataatttct gtaaaaaata gaaatagtct cactgaaaat caaaaatact
     1741 ttagttattt tcaatgagac tgtttagaaa tattgtatac ttataatgta tagtataatt
     1801 attaatcaat ttcctttact atattaatag cccatttttt agataatgct ctaggaacac
     1861 caaatataaa cttaaccact tcttcactat aagtgagcaa aacgagtaca aatataggtg
     1921 cacctttaaa tgcagctata aaagttagag gaatagaagc taaccacata catcctaatt
     1981 ctaaaaatag agcatattta gtatctcctc cacttcttaa aataccaatt ataaccaatg
     2041 tattaaaaga tttaagagcc attagtattc ccattataaa gaatatctta gttatatcag
     2101 aagctaagct atcagaaaca ctaaacattt ttaataataa tggtatattt aaaattaaaa
     2161 taattccaag tattaaacca gctgaaaata caagtataga aaacttttta gcatattcaa
     2221 tggctctttt aatatggtca gctccaagtt catttccaag cataatagat gcaccagatg
     2281 caatacatac agctgtcatt atgaagaaat tacctgtact ggttgctatc tggctagctg
     2341 ctatagcaga agtaccagct cttgcatatg caacagaata caggacagtt ccaattgccc
     2401 atacagagtc atttacaaat ataggaaagc tttttgatga aaaagatttt ataaaaattt
     2461 tatcaatagc ttttagattt tttagaccaa actttaatat atagtctttt ttataaaggt
     2521 aaacataagt aatcattaaa attaattcac atattctagc aataacagta gctagagcag
     2581 caccttttac acccaatgca ggaagaccga agttaccaaa tataaatcca taatttaaaa
     2641 taacatttgt tacaagtgca aatgcactac aaatcatacc taacttagga tttctaacac
     2701 ctcttgaacc agtactaaat acagtactta cagcaattaa tggataacta aatactgcaa
     2761 tactaaagta atctatacaa agttttacca cctcagaatc atacgaaaat atgtgtataa
     2821 ttggctttgg tgttagtaaa gcaggaataa caaatacaaa acttaaaaca attgccaata
     2881 cacaagtaag acctgtgact tttcttatat tactcacatc cttctttcca taaaactgtg
     2941 aaataaaaac tccagcacct cctgtgatac cagataagga catattaaaa agaaagaaaa
     3001 attggtttgc aactccaact gaagctacag atgtttcccc caaactactt atcataattg
     3061 tatctattac atttacagaa gtagaaatta agttctgtat gataataggt atacataaag
     3121 atagaagtat tttataaaat cttttatttt cgtcttttaa agaaaatagt tcttgcaaat
     3181 aatcctctcc tttttacata aattcttccc actcatcata tagagaggca aggttatttt
     3241 ctaaattaat tttttcttgg ctaacttctt tagctttatc tggattagag tatacttctt
     3301 cttgacataa aagtatatct aatttttcaa tttcaatctc taacttttca atttcttttt
     3361 ctatattttg tctttttatt ctatttttct tttctatttc tctttgttct ctctcttttc
     3421 ttttttcatc ttttatttga gttctagttt tttcttcttt ttcttctata atactcatct
     3481 cttggatttg ccttttttta tctatataat aatcataatt tcccagatat tcagtaattc
     3541 cattttcatc taaaactaaa attttatcaa caacagtatt tagaaagtat ctatcatgtg
     3601 atattgtaaa tatagtccca tcataattag ttaatgcctc ttcaaggact tccttagaat
     3661 caatatctag gtgattagta ggctcatcta ataacaataa gtttgcattt gatagtataa
     3721 gtttaagtat ggcaactcta gctctttctc caccactcaa tgtagaaatc ttcttaaaaa
     3781 cttcttcatc tacaaataaa aatgccccta acatatttct caaagtagtt tgagtaagat
     3841 gagtattatt ttcccaaatc tcatcaatta ttgtgttatc taaattaaga gttttttgtt
     3901 cctgatggaa gtaggaaaca tgtacattag ttccaaattt aatattgcca ctaagaggtt
     3961 gtagttcatt agttattatt ttaaataatg tagatttgcc aacaccattt gcacctataa
     4021 gtgcaacttt ttcccctcta tatatatcta aatccaagtc tttaaaaagt attctttctc
     4081 cataacccat agatacatct ctaatttgaa gaacatcatt accactctgg acagaaggcg
     4141 taaattgtat acgagctttt ttcctataag cttctggttt atctaaaacg tctaccttgt
     4201 ctaatgcttt ttctttactt cttgcacgct ttaggtgttt ttctctacca taagctttta
     4261 gtctttcaat tgattcttct tgctttttta tttctttttg ttggtcttca aatttcttaa
     4321 gctctagttc tttttctatt ttagataact caacaaattt agagtagtta ccattatata
     4381 ctttaagttt tttgttgtga acttcaaaaa ttctatttac ggattggtct aagaaatatc
     4441 tatcatgaga tattaacatg acagtccctt tatattgttt taaaaatact tctaaccatt
     4501 ctatcgcttc agagtctaag tggttagtag gttcatcaag taacaataga gttggtttct
     4561 taagaagtaa ttttcctaaa agaactctag ttttttcacc accagaaagt atgcttattg
     4621 gcttatccat atcattgtca ctaaatccta atccctttag aactcctttg gcttcagatt
     4681 tatatccata tccatttaaa tctgaaaata attctagttt atgtgaatat tcatccatga
     4741 ttttttctat aattggagag tttgtttttg tactttcttc agatatttta acctctaatt
     4801 ctcttaaata ggattccatt tctatcaaat ttttaaaaac ttctagtact tcttccaaaa
     4861 tagtattatc cgaataaaaa ttggtatttt gttctaaata tccgatttca caatctttag
     4921 aagtgtaaat atctccacta tcatatccat aaattcctga aattatttta aataaagtag
     4981 tttttccagt accattaaca ccgattatac caattttatc gccttcattt actgtaaaac
     5041 taatattttc taatatagaa tctataccaa aacttttatt taagttatta catgacaaaa
     5101 caatcataaa tttctacctc cttgaactat ctaaaattat aatgttttga tataatataa
     5161 tcaaggttat atgtattagt tttgaaaaag aaatatttta ttgtgaatca aacaaaaaag
     5221 aggacacttt taggaggtga agatgttggg aaataaaaat atatcaatgg cagttataag
     5281 aaggctccca aaatatcata gatatcttgg agacttatta gatagggata tacaaagaat
     5341 atcttctaaa gaattgagtg atataatagg gtttaccgct tctcaaataa gacaagattt
     5401 aaacaacttt ggtggatttg gacaacaagg atatggttat aatgtagaag ctcttcatac
     5461 tgagataggt aaaattcttg ggttggatcg accatacaac gcagttcttg taggagcagg
     5521 taacttagga caagctatag ccaattatgc aggatttaga aaagctggat tcgagataaa
     5581 agctttattt gatgcaaatc ctagaatgat aggtttaaag ataagagagt ttgaagtatt
     5641 agattcagat actttagaag actttataaa aaacaataat atagatattg ctgtattatg
     5701 tatacctaaa aatggagcac aagaagttat taatagagtt gtaaaagctg gaatcaaagg
     5761 tgtatggaat tttgcacctt tagatttaga agttccgaaa ggtgttatag ttgagaatgt
     5821 aaacttaaca gaaagtttat ttaccttatc gtatttaatg aaagaaggaa agtagacaac
     5881 ttaaacatat taataatgag cagttgtaaa gagtattgag aaagtcagaa gatttaatat
     5941 taaatcctct ggctttttga aatattagga taaaaattta aataatgtat ggaaataaac
     6001 aatattaagg agagtggttt ttgtggagga attatggaaa aaggcaattg aatttcatgg
     6061 tcatgaatgt ccagggttag ctataggatt taaagcagca cttgcagcaa aaaaatattt
     6121 aggatgtaaa cagtcaggag atgaggaaat tgtatgtatt tctgaaaatg atgcttgtgg
     6181 aatagatggt atacaagtag tgctaagttg cacaatgggt aagggaaatt tactacttag
     6241 gatgactgga aaatcagtat atcatttctt taatagagaa aatggaaaat ctataaggat
     6301 atatgtaaag tcaaaaccat ttgagatgga tagagaagga tacaaaagat atttattaga
     6361 aagtgatttt gaagaaatat ttgagattac agatactaag ttaagacttc ctgaaaaagc
     6421 aagaatattt aaatccctta aatgtgaagt gtgtggtgaa tttgcatctg agcaatatat
     6481 aaggattgaa gaaggaaaga aagtatgtct tgattgtttt gataaatatg aaagatttta
     6541 tgaagagtat taaaattata aattagatta aagttaaaag tattagatat gtgctaacag
     6601 tattttaaga tgaattaggt agcaacataa taaagtatat aaataatatt aattgaatca
     6661 tacataatta aaaagaggct gattagtatt tataaaataa aatcagtctc tttatgaata
     6721 acgaattatt attctggttg aggtgcatct acaggacaag ctcctgcaca agaaccacag
     6781 tctatacaag aaccagcatc tataacatat gcgtcgtctc cagctgatat acagctaaca
     6841 gggcattcag cctcacaagc accacagctt atacaagcgt cagtaatttt atatgccatt
     6901 ttgaaatcct cccttttata tgtattgtaa ttcaataaca ttataacaaa tattattgaa
     6961 tataacaatt aaattaattc tacaacaaaa actttattat gatattcttg ttttaaagtt
     7021 ttacaataaa gtttaatctg cttatatttt tatttatttt aaagaagttt attttaaaaa
     7081 taaaattaaa tt
//
LOCUS       DAKBNN010000195         7070 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000195 DAKBNN010000000
VERSION     DAKBNN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.198"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            7..612
                     /locus_tag="OUB02_003242"
     CDS             7..612
                     /locus_tag="OUB02_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCT8886971.1"
                     /translation="MIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQGARIG
                     ASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPKKDAIA
                     VFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDIDKASI
                     VASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            605..2911
                     /locus_tag="OUB02_003243"
     CDS             605..2911
                     /locus_tag="OUB02_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCT8886972.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            3050..4327
                     /locus_tag="OUB02_003244"
     CDS             3050..4327
                     /locus_tag="OUB02_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT8886973.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            4510..5232
                     /gene="yqeC"
                     /locus_tag="OUB02_003245"
     CDS             4510..5232
                     /gene="yqeC"
                     /locus_tag="OUB02_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCT8886974.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            5339..5908
                     /gene="mocA"
                     /locus_tag="OUB02_003246"
     CDS             5339..5908
                     /gene="mocA"
                     /locus_tag="OUB02_003246"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCT8886975.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            5905..6924
                     /locus_tag="OUB02_003247"
     CDS             5905..6924
                     /locus_tag="OUB02_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HCT8886976.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         2866 a          731 c         1341 g         2132 t
ORIGIN      
        1 aatattttga taggtgcaga tacttcactt agaagtttag aaattgataa aactataaaa
       61 aattactgta gtggaatact ttcaagcgct gtttctaata tagttggtgt tcaatttaga
      121 caaggggcta gaattggtgc aagtgtattt tcaaagtatg gcttttctga cttaatacca
      181 gcgcttttgg ttgttggagc gaaggtgaga ttatacaata aaggaatact agagttaagt
      241 gagtttttgg atagtgaatt aagtagggat atattggtcg aagtaatact accaaaaaaa
      301 gatgcaatag ctgtttttga ctcaataaga aaatgcacag gagatttttc tgtattaaat
      361 tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa ttggggcaag acctcaaaaa
      421 gctagattag cacttgaagc gagtaatata ttaaataaag aaaaagatat agataaggca
      481 tctattgttg ctagtaagga acttacttat ggaagtaata tgagagctag taaagagtac
      541 agaaaggata tggcaagtgc attagttaaa aggatgtata atgctattga ggagggaatg
      601 tataatgatt aataagggga taagaaagat agatagtaca gcaattgtca caggaaaacc
      661 tctatatact gaagacctca ttatgcataa agatgttttg acattaaaac tacttagaag
      721 tccacatgct catgctaaga taaagaatat agatacatca aaagccgaaa aaattccagg
      781 ggttgtgggt atttatacgt ataaagatgt accacaggtc agatattgtg tgccaggtca
      841 aacagaacca gaagcatcac cttatgatag agttttactt gaagatacag ttagatttat
      901 tggagatgaa gtagctttag tggctgctga agatgagaaa acggcactta aagccttaaa
      961 aatgataaag gtagattatg aggttttaaa gcctgtgttt gatgcaagag aagcagaagg
     1021 aaatgaagtt gttatacatc cagaaaaaga catagtagag ccataccctt ttggattgga
     1081 tgcaaaaaat aatatagtat caaaagaatt ttttgaattt ggagatgttg aagctaagct
     1141 taaagaaagt gattttgtgg ttgaatcttc atacaaaacc caaagtcaat cgcattgcat
     1201 gatggaaact cataggtcat attcatataa agatataaat gatagaattg ttgtagtttc
     1261 atcattacaa tcaccacaca atgtaaagag aatacttaga aaagtattgg gtataccatc
     1321 ttcaaaaata agagtaatta aacctagaat aggtggtgga tttggaggaa agaatatagc
     1381 agcaacagat atatttactg catttgttac tatgaaaact ggtagaccag caaaatttgt
     1441 atttacaaga agtgaaactt ttgtaggttc taatagtaga catgaaatgt tttttgatgt
     1501 taaagttggt gcagataaag aaggaaattt aaaagcaata gagctaaagt cattaaacaa
     1561 tacaggtgct tatggtggga atggtgtctc tgtttcttcc gaatctggac acaatacctt
     1621 accaatatat aatgatattg atgctgttag atttaatgct agaacagtat atactaatag
     1681 gttaccagca ggagcactta gagggtatgg tgctacacag ggaatatttg caatagatag
     1741 tgctattgat gaattggcac ataagattgg aatggatcca cttgaattta gaattaaaaa
     1801 tataatagat aggtatacca atggaaaagc agctgaagac gaaatattaa gttgtgagct
     1861 gagacaatgt atagaaaagg gcaagaaatt aattaattgg gatgataaat atccatgtaa
     1921 agatatagga aacaacaaag taagagcagt aggtatggca gtagcaaatc atggttctgg
     1981 tataccgcgt gtagatatgg caacagtcac tataagaatg gatgaagatg gcacatataa
     2041 actattatct ggtcttgctg acttaggtca aggggctgat acaattcaag ctcaaattgc
     2101 tgcggaagct ttaaatacta ctgtagatag aatttctatt tatacaggtg attcagactt
     2161 atgtccctat gatacaggag catttgcttc ttgtacgaca acggttacag gaaatgcaac
     2221 aataaagaca gcaaataaat taaaagatat attactttca gttgcagagt caaagctagg
     2281 atttaaaaaa gaagatttag agttgggaaa agacagggtt tttgttaaaa acgataatag
     2341 caagtttgtt ctattagagg aattgggtag agaatctgtt ggaggaatag gatttgctag
     2401 tgatgatgta ccagtagttt tggaagcaac agaatctttt ggcatgttga aaaaaataaa
     2461 accatttata gcaggatttg cagagataga attagataaa aatacaggcg aatataaagt
     2521 cataaatttt gcatgtgtac ctgattgtgg aacagttata aatccaactt tagctagaat
     2581 acaagtagaa ggtggtgtga tgatgggtat agggtttgca atgtatgaag accctatatt
     2641 tgataaagat ggtagattat taacagatag tttcctacag tataaagttc ctacaaaaaa
     2701 agaagttgga aatatattgg tagattttgc tgagaatttc gacccaggag gtccttatgg
     2761 tgctaaatca ttaggagaaa tagttataca tacaccagca ccagctatag caaatgcaat
     2821 atttaatgct acaggagttc gtttaagaga gcttcctatg acttttgaaa aagtttatat
     2881 gggtatgaaa aataataata aacaattata ataaataata agatgtataa aagcaagtaa
     2941 atttaaatta ttttgtaaaa taaagtaatt aaatggacat tatatatggt gttttgtaaa
     3001 aataccgtat ataatgtcta gtagagtata ttctaaagga ggtgagttca tgtcaaacag
     3061 aataaaaaat atacactatg gaactcagag acaacaatta aaaaacttaa ctaaaaaaga
     3121 atatcaagct ctacgagaaa tgtgcttctt agctaagaac ttatataatg ttggtacata
     3181 taatgtaaga cagcattatt tcaatcaagg agaacatctt aactatgaag ctaattatca
     3241 tgtatgcaaa gaaaatgaga actataagct tctcaatagt aatatagcgc aacaaatatt
     3301 aaaagaagta gacagttcgt ttaggtcatt ttttggatta attaaattag ccaaagaagg
     3361 aaaatatgac tttagaggga taaaatcacc taaatattta gataaagaag gattttttag
     3421 cataataata ggtcaaataa gaatcaaaga agatgggatt ttaaatgtac ctatgtctcc
     3481 agcatttaaa aaactatatg ggaaagtaag tataaaagtt ccacaaaata tactagagaa
     3541 aaaaattaaa gaaataagaa taatacctaa acataaagct aggttctttg aaattcagta
     3601 ttgttatgaa ataccaaaat cagaggaagt ttcaaatcaa aaaaatgcac tagctataga
     3661 cttgggttta gaaaatttat gtacatgtgt taccaattta ggtgatagtt ttatcataga
     3721 tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa cagaatgata aaatagaagc
     3781 cttaaaatta aaaaagaata taactgcaat aacaagaaaa caatttaaag tttggaataa
     3841 gagaaataat caagttaaag actatgtaaa taaaacctgt ttttatataa ttgattattg
     3901 tgtaaaaaat aatattggaa atttaattgt aggatataat gctaagttag aaaaaagtga
     3961 taatatggag agaaaaatat atagaaattt tataagaatc ccatttggag agataaaagg
     4021 aaaattagaa tatctgtgta aaataaaaaa tatcaatttt attaggcaag aagaaagcta
     4081 tactagtaaa tcagattttt ttgctgatga ttttctacct gatatcaatg tggacaatgt
     4141 taaaaaatat gactttaaag gggaaagaat aacgcgtgga cagtacaaat ccagtgttgg
     4201 aatcattata aatgcagatg taaatgcgag tcttaatata ctaaaaaaat cacaagtagt
     4261 taatttatct aatttacaga ataatcctca aatattaaag caaccacaga gagtaagaat
     4321 atcttaatta tatttaaaag ataaactgat agcgtaagct tacagcttag cggcgaattg
     4381 gatccgcctt gttgtttaca acagaatcta tttactttcg agtttatgga atttcaaaaa
     4441 tcacttttac taaaaaggtc taactataat aattaagaga ttagaaaact ggttataaga
     4501 ggtgaaagtg tggaactatc aaatataata gaaaagaatg aaataataac tgtagttgga
     4561 gcaggaggaa aaacttcgct tataaactat tttgcaaatt tttatagaga taaattgaaa
     4621 gttctcctta caacaacaac taaaatatat gtaccaaacg attatgacaa tataattatt
     4681 acaatagatg gaactgttat tccatctatt tgtcatggaa taacagtctg tggcagttat
     4741 ataaataatg aaaataaatt agtaagtatt gattcctcta ttttggatga aattgtagac
     4801 caatttgatt tagttttaat agagggagat ggctcaaaga gaaaaaaact taaaggttgg
     4861 aatgctaaag aaccagtagt atatcataaa actacaaaaa ccatagggat attagatata
     4921 acatcatttg gaatgaatat taatgaagag aatatacata gagtagaaat atttaaaaaa
     4981 atagcaaatt tagatacaag tagtattaat agtagttcta cagtaagtat tgaaaactta
     5041 aaaaatatag tattaaatcc aaatggatta tttaaaaatt attctggaaa aagagtatta
     5101 tttattaata aagttgaaaa tgaaaaatat aaaaatttag ctataaaatt aatagaaaat
     5161 attaaagaat atgaaagtga aatagagata ttttatggaa gtgtaaaaca aaaattttgt
     5221 gtaagatatt gaagtattaa aatagtttat atgatattat atatatagat aatatcaata
     5281 gagatattat aaaaatcata gaactatcaa tatattataa ataccaagga tgatatatat
     5341 gataaatgca gtgataatgg catcaggctt tgccagaaga atgggagcaa ataaactttt
     5401 attaaattat aaaggctgtt ctataataga acatgtattt agagaagtta gcaagattga
     5461 ttttcatcaa gttgtagtag taagtcaata tgaagaggtt ttgttattgt gtaataaata
     5521 tggttttgaa tatatagata ataaaaatgc aaatataggt caaagtgaat ctataaaatt
     5581 aggagtttta aatagctcaa aatgtgatgg ttatatgttc tttgtaggag accagccatt
     5641 tattgataac ttatatatag ataaaattat aaatactttt aaattagata gagattttat
     5701 agtaattcca agatacaaag atatgtgtgg aaatccagtc atattcccat atagcaagaa
     5761 agaagaattg ttggcactta aagaagatga aaaaggtaaa accattttaa aaaattcatc
     5821 aaaaataaag tatgtagaag ttcctaaaga aatgttattt gatatcgata caaaaatgga
     5881 ctatgagaga attgggggta aattatgaag ctaataaaaa ctatagatgc tgttggacaa
     5941 gttttatgtc acgacataac tcaaataata cctggagaat ttaaaggaag aaaatttaaa
     6001 aaaggccaca tagtaaaaga agaagatatt ccagtgttat tatctcttgg taaagataat
     6061 ttgtatgtat gggaaaagtc agaaggaatg gttcatgaaa atgaaggcgc attgttttta
     6121 aaagaactaa ctgctggaga aaatttagat tttagtgaaa taaaagaagg gaaaatagat
     6181 tttatagcag catgtgatgg tctattaaag atagatgttg atgcactttt tgatttgaat
     6241 tgtgttggag aaataatgat ggctactctt cataataatt ttatagtaaa taaaggttta
     6301 aaagtagctg gaacaagagt gatacctttg gttatagatg agaaaaaatt agaagatgca
     6361 aaaaaagtag ttggtaatag aaaaatagta aatgtagtac cttttaaacc taagaaagta
     6421 ggaattgtta ctacaggaaa tgaagtgttt tatagcagaa ttgttgataa atttggaccc
     6481 gtaatagaag aaaaagtaaa aggctttggt tgtgaagtta taggacagac tatatgccca
     6541 gatgataaag agattataaa gagtgcaata aaagaattta taagtcaagg agcagaactt
     6601 atctgttgta ctggtgggat gagtgtagac ccagatgatg ttacaccaac agctataaaa
     6661 gaaactggag ctgatttagt gacatatggt tcaccaatat taccaggagc tatgttttta
     6721 cttgcttatt atggagaagt tccaattatg ggtataccag gatgcgcaat gtatcacaaa
     6781 actacagtat ttgatatagt tttatctagg gttttaattg atgaaaagct tgataagtat
     6841 gatatagcta gatatggtca tggtggcctt tgtatgaact gtgatgtatg tacttatcct
     6901 gcatgtaatt ttgctaaaat ataaagttat taaaaaagag tatttacaaa ttaagtactc
     6961 tttttaaata tgaaacaaaa cagctagaat gatgttttaa gaaaagcata tttatttaga
     7021 aattataata acttattttc aatgtgtaaa agaatgaata aagttgcaat
//
LOCUS       DAKBNN010000196         7045 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000196 DAKBNN010000000
VERSION     DAKBNN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.199"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..509
                     /locus_tag="OUB02_003248"
     CDS             <1..509
                     /locus_tag="OUB02_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886977.1"
                     /translation="YISESKGLSKRLYSMPITRVQFLIYDFVGLWIYSFIFLLLYVLF
                     FRILGITFKGNFAILLLLCALSSYFMTSISTFVTSFFSKKYGTAIVYALLFLQTIFGG
                     IFSMISDTFTKLTSLSPTYLIGELFSNYETFKTIDSIGDLIFTCLIISTILIALAIVK
                     EKYKWREV"
     gene            512..1576
                     /locus_tag="OUB02_003249"
     CDS             512..1576
                     /locus_tag="OUB02_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886978.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVFDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            1820..2317
                     /locus_tag="OUB02_003250"
     CDS             1820..2317
                     /locus_tag="OUB02_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HCT8886979.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(2493..2741)
                     /locus_tag="OUB02_003251"
     CDS             complement(2493..2741)
                     /locus_tag="OUB02_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886980.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(2807..3823)
                     /locus_tag="OUB02_003252"
     CDS             complement(2807..3823)
                     /locus_tag="OUB02_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCT8886981.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(3881..4648)
                     /locus_tag="OUB02_003253"
     CDS             complement(3881..4648)
                     /locus_tag="OUB02_003253"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCT8886982.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(4716..6905)
                     /locus_tag="OUB02_003254"
     CDS             complement(4716..6905)
                     /locus_tag="OUB02_003254"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HCT8886983.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         2269 a         1215 c          738 g         2823 t
ORIGIN      
        1 attatataag tgaatcaaag ggactttcaa aaagacttta ttctatgcct ataactagag
       61 tacaattctt aatttatgac tttgtaggtt tatggattta ttcatttata ttcttattat
      121 tatatgtttt attctttaga atacttggaa taacatttaa aggtaatttt gccatattgc
      181 tcctactttg tgctttatca tcttatttta tgacttcaat atctaccttt gtaaccagct
      241 tttttagtaa aaaatacggt acagcaatag tttatgcatt actcttttta cagacaattt
      301 ttggaggtat cttcagtatg ataagtgata catttactaa gcttacaagt ttatctccta
      361 cctatttaat aggagaattg tttagtaatt atgaaacatt taaaactata gattcaatag
      421 gagatttaat tttcacatgt ttaattatct ctacaatatt aattgcactt gctattgtaa
      481 aggaaaaata taaatggagg gaggtttaat aatgattaga ttaactattg caaatataaa
      541 aagatatcta aaaaatcata ctcttttaat taatatggtt atgcttccta ttatactaat
      601 tttttcactt aattttttta tcaacaactc aggaaatcaa tcaatgtact tttctccagt
      661 agcaattgta tttgactctt caggaaagta tgaacataag ttaataaata gttctaaatt
      721 aaaagaaaat tcatttagat taaatgaaca agataaggct atgaatctat taaaaaataa
      781 taaggtttct gctgtattcg ttttagataa aaatttttca aatagtatag ataaattaca
      841 acgacctgta gttaaatgct ttaaaattga aaatggtggt ggtagtcttt gggcagaatc
      901 acaaatagaa tcttttatta ctaaatcgtt aaaattgaaa gtggataaaa atatagatga
      961 taaattgaca aagacaaata taattgataa caaaccagat aagaataaag gttcattttt
     1021 agtagtcttc ttgatatgct attttatgta tattaatgcg gcacatcttg cttctgattt
     1081 atttacatta aaaaaatcaa atgtgctaaa aagacttatc tctacagata ataaagatat
     1141 aaaaataatt ttcagtatat ttttaggact attttttatt caatcaattg tatacacttt
     1201 tgctctatta tgtttttcaa ttatagaaga ctttaatttg agtctgaatg tattgatgat
     1261 tgtactagca aatagttttg tttccactgg atttgtattt tgggtagcta gagtatttaa
     1321 aaatgaaagt tctataagtc ttatatctac attttattct ttaataggtc tagctatatc
     1381 tatctctagt ctaataccat caatggataa attatccttt atgactaatc tttcaaagtt
     1441 cacaccattt tattggacta tagatgctat taaaaacaat ggtagtattt taataaatat
     1501 aatagctcta atattaattg gaattatatt tgtaactgca ggaagtctta aactaagaga
     1561 ttttgctaaa aactaataat tattaaatat aattagtatc tatttttata taattgtaaa
     1621 agaatatttc aacatattaa attattttat cttatatttt tctctttagt ttcacacatc
     1681 accaagtcta aacttcatat tatatattaa tatttttgac ttgtcaagtt atttatgtta
     1741 taatattaat cattagataa ttaaagcatt gtacctaagt tgcaatatct aaaattcaat
     1801 ttggaggtat aactagcata tgtataaaat tggtgaaagt gtaatgtatc caaaagaggg
     1861 agcctgctct gtaaatgata ttgtaacaaa gaaaataaat catgagatgc aaaaatacta
     1921 tgaattaagt gtcattttta atagtaacct taaaatttcg attccagttc taaatgcaga
     1981 tagaattggc atacgtccag ttatggatgg aaatgatgtt gataatttta tccaatctat
     2041 taataaaaca gatggtgtat ggatttttga tagaaaagaa agacttaaac tgtaccaaga
     2101 taaatttcat tcaggagatg tttttgaaat tgtaaaatta atcaaaatgc ttatgataca
     2161 agacagttca aaacaattgt gtagcacaga taaggagttt ttaaataaag ctcaaaaatt
     2221 tgctttatca gaattagctg ctgctcaatg taaatcctat acaatggtat tagaggaaat
     2281 gaaaaaacat atattaaatt caaagaatac aaattaaacc agttttttaa actggtttta
     2341 ttttttatac taatcatcca tgctacttta gcttatttta aactgcctaa tctaatatta
     2401 ataaagttat ctcaattaga tagatgtatt ttattcaaat aaaaaaaact aagagtaaat
     2461 catattactg atttactctt agctaataaa atttaatctt cttcaagttc ttttttctct
     2521 tgtaataata cacttaattc atttgcttct aaaacttgtg cctctgcaat atcaagtaac
     2581 tcttgttgcc attccacaca tttatctttg taatcaaaag gtagcgttct gtcaactatg
     2641 tacttatcaa tacagtcctc cgcactttta attatttctt ttctttcatc aataaatttt
     2701 tctaattcta ctaaggcttc ttcataagct tttctactca tataaattcc ccctttgtac
     2761 aattattcta cattaaaatg atttttcctc ttttttgtat aaaattttat ttgtatataa
     2821 cttttccatc aatatctttt atagtttgac atccagttaa tatcatagaa ctacttagct
     2881 ctgattgaag tttatttaca taagtttcta caccatcagt tgcaccacca aaagaagcag
     2941 tcacaaaagg tcttcctatt aatactgcat ctgcacctaa acctatcatt tttagtacgt
     3001 ctaaaccagt tctcactcca ccatctgcaa gtattgtaac tttaccttta acagcatcag
     3061 caatttttgg taaaacttca catgctcctg gtgtacagtc aagcactctt cctccatgat
     3121 ttgatacaac tattgcatcc acacctgctt ctactgccat taaagcatct tcaacagtca
     3181 ttataccttt caatataaat ggtagtttag tagactttac taactccttt atttgtttaa
     3241 catttttagg aagtactggt tttccatgta atgataaagt tactagacca caggcatcta
     3301 tatcaactcc aactgcaaat gcaccagctt cttcagctaa tcttatcttt tttataatat
     3361 tttcattatc ccaaggttta ataaatacaa ttccagctcc attatacttt ttgactacat
     3421 ctaaattttc cattaaaaaa gcatctactg ccgtatcacc aaccattgca taaataccac
     3481 tattagcaca tcctgcaact actggttcta tgtaatctct ttcattaatt tttccaccca
     3541 tatttaaagt agttcctgtt actggagctg caaatatagg tgcactcatt ttctttccaa
     3601 acatctctat agatgtatca ggatttgata catcatgtat tactctcata tttactttta
     3661 ctttttctaa actcttgcta ttttctataa aagaagaacc acttccttta cctcccatgc
     3721 caggcacttc acctgcacaa gcaaccccat tacaaacctt acaaacttta caacttccat
     3781 ttaaattttc tctagcactt tttaataatt cattatattc catctttaat cccccattaa
     3841 ctatagtaat ttatattata tacattatat catattatat ttacagtata tttttcaaaa
     3901 aactttttct gtggatagga gttatgccat atttttcaat agcttcatag tgttctttag
     3961 ttccatatcc tttgtgagat ttaaacccat attctgggta tactctatca tattggtaca
     4021 ttatactatc cctagtaact ttagcaagta tacttgctgc tgctattgat atagattttg
     4081 aatctccttt cacaattgga ttttgagata tgtcaatacc tggtattgtt gctgcatcaa
     4141 ctaataaata atctggtgct ttttttaaac aatttatagc ctttttcatt gccatatatg
     4201 tagcatttaa tatattaaat tcatctattt cttcattgtt tactatacct attccataat
     4261 ctagtgcttc ttcttttatt acttcaaaaa gttcatctct ttttgcctca ctcagtttct
     4321 tagagtcatt tacaccttct attttggtat cttttttaaa tactacaaca gaagctacaa
     4381 caggtccagc aagtggccct cttcccgcct catcaattcc tccaatatat aaataacctt
     4441 tatcatagcc ttcgttctca aatatattta tagtttccaa tctttcttct tcttttctaa
     4501 tattatccag cttttttgcc agttttatag ctagaccttg tacagatttt ctttcatcaa
     4561 ctcttaacag ctctatatat tccatatatt tttctacttc taaagtctct attatttctt
     4621 taatctccct cacactttta tcctgcatac cattccctca tctttcgttt ttaaaaattt
     4681 aatttttaaa tatattaaaa ttttttaatt agagtttaat tgcatccact atagccacat
     4741 tttaaacata tactacatcc acctgtatgt gcaattcctt cacttccaca ctcagggcac
     4801 acaaattttt catcttcttc aaaattttta tttattattt ctttggcttt ttcatcttta
     4861 ttatcttttt cttttttatt atttttataa tattcttttt tactattttc ttttttatct
     4921 tctttattat tttcttcctt tttttcattt attaaaattc cactcctttt acatccatct
     4981 ctaaatattg ttactccttt aagaccagcc tcccatgcct taatatatat attgaaaaca
     5041 tcttcaactg tagtttcaaa aggtatattt attgtagatg atatagaagc atcaatatac
     5101 ttttgccata ctttttgcat gtttattctt tcttcatatc ctatattcat agcagtaaca
     5161 aaaaaatctg gtaaacattc ttcatctgta atacctttct tgtccatata ctctttaaca
     5221 attggagtat atactttata atacacatcc tcatcatgca agctttcagt ttttcttata
     5281 tatgaaagat taaatatagg ttctattcca ccactgatac ctatcattgt agatatggaa
     5341 cctgtaggag gtatggttaa aagttgtgaa tttctaagcc catacttttt aaccatttcc
     5401 ttagtgtctt catttagatt ttctataaaa aacttagatt tagaaatatt ttcatattta
     5461 tactcactaa atgttccata ttctttagca attaatgctg attgcttcac agcttcattt
     5521 aacatagtct taccaattaa atcacatagt tctaaagatt catcagaacc atatcttatt
     5581 cccattttta taagcatatc agctataccc attacaccta aacctatctg tctatactta
     5641 tgtacactat ctctttgttc ttgtaatgga tgcaagttca atccctcatc taagacatca
     5701 tttaaagcta ctacagcttc tctaacgcaa gattttaatt tattaatatc aaaagtagca
     5761 tgttcaccaa atggttgtat tataaactct gacaagttta ttgagccaag tagacaactt
     5821 cctccacttg gaagtggttc ttctgcacaa ggatttactc ctgaaaagca aaagttctta
     5881 tcttcactca taaaattata tgtttttatt ttatcccaaa acaatatacc tggttctgca
     5941 taatcccagt tatttttcac aaatttatta aataaatcat aagcatttac tatcttaact
     6001 attttttctc cagtagtttc aacttcaaaa gtacaattat ataacttttt ttctcttaca
     6061 gcttgcataa attcatctgt tactcttaca gaaatatttg ctttagtaac cttctccaaa
     6121 tctgttttta tatctataaa gtcttctatc tctggatgat ttacatccat agaaatcata
     6181 agagctcctc ttctccctct ttgaccaatt aagccagttg tcatactgta aagttccata
     6241 aaagagactg ctccagtcgt atgttttgca gcattgttaa ctttcgctcc ttttggtctt
     6301 agctttgaaa tatcaattcc aactcctcca ccataactaa aagttctagc aagttttttt
     6361 gcagtatcaa atatatcttc tatgctatct tttggtggtt ctattacata acaattagaa
     6421 tatgttactt tttttccctc tttctgtaat cctctatttg ctaaaattct tccagcaaat
     6481 agaaatcttt tttgtcttat tagttttgat attcttgaac tattatttga aattctcttt
     6541 atccattcat caaaactctc accattatgt ctatacttgt tcttccatat tgatttttgt
     6601 aattcatcaa actcccaagc tttagtcctt atttcagagc gtctttctct atataatatg
     6661 tagtgttttg ctatatcttt tctatttgat tccataagta gtacttcaac tatatcttgt
     6721 atttcttcta cggataagca tttttccata gaattatagt cattagctat ttttcttgat
     6781 atatcatatg ccaactctct atctacgcca ccttcactat ttatactagc tttatagaca
     6841 gcatcttcaa tcttactttt actaaaaagt tcttttgtac catctctttt tattatgtac
     6901 ttcattttta aacctccaat aaattagtat taaattaata cacacattaa ctataactct
     6961 aaacaaactt tttatatatt tgtatatttt catattaatt atctaacata atatatatta
     7021 tactatttta tattcaatat aacac
//
LOCUS       DAKBNN010000197         6946 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000197 DAKBNN010000000
VERSION     DAKBNN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.201"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(308..697)
                     /locus_tag="OUB02_003255"
     CDS             complement(308..697)
                     /locus_tag="OUB02_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8886984.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            889..1335
                     /locus_tag="OUB02_003256"
     CDS             889..1335
                     /locus_tag="OUB02_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886985.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            1599..2324
                     /locus_tag="OUB02_003257"
     CDS             1599..2324
                     /locus_tag="OUB02_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8886986.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            2311..3555
                     /locus_tag="OUB02_003258"
     CDS             2311..3555
                     /locus_tag="OUB02_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8886987.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            3635..4312
                     /locus_tag="OUB02_003259"
     CDS             3635..4312
                     /locus_tag="OUB02_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8886988.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            4313..6874
                     /locus_tag="OUB02_003260"
     CDS             4313..6874
                     /locus_tag="OUB02_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8886989.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
BASE COUNT         2901 a          666 c         1105 g         2274 t
ORIGIN      
        1 ttttttatgt gtatgaatat atagtatact ttcatataat aaaaaaccta taataccaat
       61 aaaataggaa agttccaatt tattaaatgt aaaaaccaga ataaaaacta atataaagtc
      121 tataataaaa cctaaagata atagtttttt aaatttttta tctaacttta taaagtaatt
      181 ataattttta tgctcttggt ctatagaaac tccatagatg tgttttttac ctacaaaata
      241 gggcataaaa taaaatgtag catacatcaa aattagaact ggaaatattg taaatagaca
      301 ttcaatctta ctcataatat ttcacctcat acttgtcaaa taaacttgaa cttagtgaca
      361 aaaattcttc tttactaata cctgataaat aagattttgc caccaataat tccaagtcaa
      421 gtttattttt ttgacgagtt gtattatcta ttttcattgg tagttgagct acaatagcac
      481 cttttcttct atcaatattt aagaatccct cttcttttaa ttcattgtag gatttgttca
      541 ctgtatgtaa atttacacca atattttcag ccatacttct tacagatgga agtgattcat
      601 ttattttcaa ttctcctgaa gctatagcct tagttatttc ttctttaatt tgtacataaa
      661 tagatttatc actattaaaa tctaaattta aaaccataaa aaacctccat tatgctttat
      721 aaatgaaaac gattgttata tacatagtat aacataaaaa ataataacaa acacaaaata
      781 taagtttata ttttgtaaaa attgtaattt aaaaaataac ataattttta ttaaaataat
      841 acagatacta tttatagata gaaataaaaa ttagaaagga gaaaaaatat gttaacatat
      901 ttaatgaaaa gacaaatgca taaaaaagat gatggaatga gtaaaccaat gatgattaca
      961 ggagctgtaa ttacaggtgt aggagcatat gctgtatata aaatggtaaa aagccataaa
     1021 tctaattctc aaatggtcaa tacaaattat tatggaaata gcgattatga tgattataaa
     1081 tatgacgaat ttgattatga ctgtgattgt agagacacaa aacaagatta tgaatatgaa
     1141 gaattaaaaa aaaaagtaaa agaatttaat tcaagaagaa taaattgtga aaattgtcca
     1201 catgtttctc aagaagaaat gatgcaatat gtaaatagtg ttaaagatgt aaaggaagag
     1261 attgatttac atgaagatgc taaaaaagat aatatttaca aagaagatga atacaaaaaa
     1321 tatgaagaag aataagtaat attttgtata actgaacaaa tagtaatgtc aaattattta
     1381 atatctagtt ttaatatgta agttgtaact taacttgaaa gaatcagtat cattctttag
     1441 atagttaagt aacaacttac attttatttt tataaatttt aatagttctt tgtgactatt
     1501 ttgtaatttt agaaatattt tagtatccat ggtaattgtg tataataaaa cttgtataga
     1561 tagatagtat gaaattaagc agatagttta aaattaaaat gaaaagaaag gaaatagtta
     1621 tgttaaagtt gttgatagtt gaagatgaca gtactatatc ttttggaata aagtatgctt
     1681 tagaacaaga aggatttagt atagatatat caaaggattt atctagtgga aaagaaatga
     1741 tttcttctaa tgaatatagc atgatactgt tggatgttac attgccagat ggaactggtt
     1801 atgagctttg tcaatatata agaggattct ctcaggttcc tataatattc ttgactgctt
     1861 gtgatgaaga agtaaatatt gtaatgggtc tcgatatagg tggagatgat tatataacaa
     1921 aaccttttag aattagagaa ttaatatcca gaataaaagc tgttttaaga agaaaaggta
     1981 atacttcaga agaaaataag aaaatactta aatttggaga tttaagtata tatacattag
     2041 aagctagagt atataaaaat gataaagaaa tatttctgac atcagttgaa tataaacttc
     2101 ttttaattct tatacagaat aaaaatactg ttttaagtag aactcaaata ttggaaaaac
     2161 tttgggatgt aacttatgat tttgtaaacg acaatactct aactgtttat ataaaaaggc
     2221 ttagagaaaa aataggtgat gatttatgtg aaccaatcta catagaaact gtaaggggga
     2281 ttggttataa atggatagga agtgaaaata gtgtttctat ataatccaga agtgaagaag
     2341 tttttaagta agtatgttac tttgatgttt attgttatca taatatctat tggatttagc
     2401 ataatcaacg tgtccttaac taaagagatg gttgtaagaa ataatcaggc tataatagga
     2461 actttatcaa gcaagtatcc aaacttggaa agtcagatag tagacataat aactcaaggg
     2521 aagtctatgg agaacataga ttatggtcag aaaatattaa ttaagtataa ttatgataaa
     2581 tctattagga taaactctga gcctattatt tctaaattag ttttagagac tatcaaaata
     2641 aatataatat tagtatgtat aatatttatt ttgatatttg tgttggttgt tagatatttt
     2701 acgtcaattt acaatgattt atcagatatg actaagtatg tttactatag ttcagagggt
     2761 aagccatttg atatgaaaaa caagaatcaa gaagggcaga taggtcttct aaaaactgaa
     2821 ctcttaaaga tgacaacaat tcttaatgaa aaggttgaac ttttaaaaag agaaaagata
     2881 tttttaaaca atacaatttc cgatatatca catcaattaa aaactcctat gacttcttta
     2941 ataatgttga atgatttatt atataatgat gtaccatacg aagtaaaaat cgatttttta
     3001 aataagataa aaaatcaatt aaatagaatg gattggttga ttaaaagcat gcttaaatta
     3061 tcaaaggtag aagcaaaagt gataaatttt aaaaaagata aggtaaaatt tagtgaactc
     3121 atacatagag caatgctatc tatgaaaata cctatggaga ttaaaaatca aaaactgact
     3181 atagagggaa atgataatgt atcttatatt ggtgatattg attggtccgt ggaagcatta
     3241 gtgaatataa tcaaaaattg tgtagaacat actcctgaat ttggaaatat aattataacc
     3301 tataaagaaa atccattatt ttctgaacta ataataaaag atgagggtga aggtatagat
     3361 aaaaaagata taccacatgt atttaaacga ttctatagag gaagaagtag ttcaaaagaa
     3421 gatagtgtag gaataggtct tgcaatgtca aaatctataa tagaaagcca aaatggcgat
     3481 atatatgtaa acagcgaaaa aggtaagggt acagaatttc atataatatt tcataaaatg
     3541 tatgatagtg actaatctgt aatatttaat tcactttata gtaatagcca aaacctaata
     3601 tatgaagtat agaatataaa tattggaggt agttatggaa attttaagag tagaaaatct
     3661 aactaaaagt tatggtaaaa atgaaacaaa agttgatgct ataaaaaatg taagtttatc
     3721 agttgaaaaa ggaacgttta tagcaataac aggtccaagt ggaagtggta aaagtacctt
     3781 attacattta ttaggaggag ttgacagacc aactagtggt aaggtttata taaatgatgt
     3841 tgatatatat aatttaaaaa ctaaggattt ggcgatattt agaagaagaa atataggtct
     3901 tatatatcaa ttttacaact taattccagt cttaaatgtg aaggaaaata tattgcttcc
     3961 agcagagctt gacaatagag atattgatag agaatatttt gatgatttaa tgaaaacatt
     4021 aggtctaatt gatagagaaa agcaccttcc aaaccaatta tcaggaggtc aacaacagag
     4081 aacctctata ggacgtgcat taattaatag accttctata atattagcag atgagccaac
     4141 aggtaattta gatagtaaaa attcaaagga aatattagaa ttgttaaaat tgtcagtaaa
     4201 aaaatacaac cagacactaa taatgataac tcatgacaaa aatatggcac ttcaagctga
     4261 tagaattata agcatagaag atggaatcat aaaaagtgat gaggtgatgt agatgaattt
     4321 atatacatca ctaactatta gatacttaaa acaaaataaa agaagaacca ttgtaaccat
     4381 aattggtata atactttcaa cagctttaat ttgtggtata ggaaatatat ttgaaagttt
     4441 tatggattat caaatgagag aaactataaa gaatgatgga agtttccatg ttacatttta
     4501 tgatgtaaat agaaaaaatg tggaatatgt aacgaaatca gctgaaatag aaaaacatgc
     4561 atttaccaaa caacttggat attctaaatt agaaaatagt gaaaatgcta tattaagtat
     4621 aaagcaatat gataaaaatg caataaacgg atataaagtt tctataaaag aaggtagatt
     4681 tccagctaag gaaggagaaa ttgttttaag tgaaagtatt attaatctta tagatgataa
     4741 gttaaaaata ggagataaga taactttaaa agtaggggat atgtttgact caagcaagaa
     4801 aaagattgat agtatgacat ttcatgaagg tgattatata gcaaatgaaa aagatagaac
     4861 ttttaaagtt gttggtataa ttgaaaaacc tgggtttgaa cgttataatg gaattgcaac
     4921 agctatagcc tactttaatc ctatgaataa ttacaatgat acaattaatg tatcagtagc
     4981 agtaagaaat cctaaagatg tgtataagat aagtaataaa ataagtaaaa atatatattc
     5041 aaataaagat aaagaagcag tttccgatat gataaaatac aatgaacatt tattgaggtt
     5101 acaaggtgct agtaagtatt caaatataaa tagctctata aagtctataa ttatagcagt
     5161 aactatttta gtaatcatat gtacaattgc tacagtgtac aattccttta gtatttctat
     5221 aaatgaacga aaaaaacagt ttgggatatt aaattctata ggtgctacat ctagtcaaat
     5281 taaaagatta gtttttatag aaggtattat aattagttta attggtatac caataggact
     5341 aatatctgga acagtagcta tagacctatt gtttaaaatt ataaataagt attttactga
     5401 atcggttgtt actaagatga gtttgcagat agtgtataat ccaataataa ttattgttag
     5461 tataataata gttttgctta caatctttat atcaattcta ctacctgcaa tatcggcttc
     5521 taatatttct ccccttaatg tgattaaaaa tactggagag tataaagtgg gcaaaatcaa
     5581 aagctcaaga cttattaaaa tgatttttaa aactgaagga gtacttgcat ataaaaatat
     5641 taggagaaat agaaaaaaat ttataataac tcttttttct ttaatggtaa gtataataat
     5701 atttatatct tttagtggct ttacattact tttactaaga ggtgaagaaa ttagaaattc
     5761 tcaaagaaaa tatgatttgt atttaactgc aaagaacaca gctaggtctg tagaggatac
     5821 tataagtgaa ctagaagata ttgatgggat aaaaaacttt gaattggcaa caggacaata
     5881 tgtgtctata agagtaagtg aaaataaaat aaataaatcc aaggaagatt taatcaggaa
     5941 atattatcaa aaatataaga ttggagatag ttatgaatat gatttcagta ataatgaatt
     6001 aatttttcca ggagattttg cagtaaaaaa caaaataaat aacttagtac agggctcttt
     6061 taataaagag agagcaattg aagaaaatgg tgttatatta gttagaaaaa gtgcatttga
     6121 agaaaatggg aagaaaggtg tagtagaact taccaactat aaagtaggag atacagtaaa
     6181 ttgtgaatat cttgatgaaa atgccttaag taaaaaagta aaggtcaaga ttcttgctat
     6241 aactgatgaa gaacgtctag gtcttggtta tcaaaatatg gggttacaat ttataactta
     6301 tgatgaagtt gctaaaaatt taaatttaaa attaagtaaa agccttatat ttattgattc
     6361 aggaggaagc attaagacta agaaggaaat agaagctttg gcaaataaaa ataattttaa
     6421 cttttatgat gaaagtgcaa gcaatgaaag tgaaaaacaa gatttaaagg ttattaaaat
     6481 atttgtatat ggttttatag tagtgatatc tttagtgagt gttacaaata ttctaaatac
     6541 agtaagtaca agtataaatc taagaaaaag agaacttgca gtaatacaat ctataggggt
     6601 tactcctaaa ggatttagga aaatgatata tttagaaagt tttatatatg gtatactgtc
     6661 attgttgttt ggaataccaa ttagtattgg tataattcta attatgaata aacttatttc
     6721 aggtgtaatt gaattttctc cgattatacc gtggacagcc atagttatct gtatagtaag
     6781 tgtttttata ataactttta tagcagggta tataccaatg agtaaattaa ataaagaaaa
     6841 tataatagat aatataagaa gagaaagtat atagatataa aaataattct taactatata
     6901 aaatattatt aaaatcattt aagtggtata aaatattatt atatca
//
LOCUS       DAKBNN010000198         6915 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000198 DAKBNN010000000
VERSION     DAKBNN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.202"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            15..2135
                     /locus_tag="OUB02_003261"
     CDS             15..2135
                     /locus_tag="OUB02_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8886990.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNVKKYQFNIGKDKNIMEYDYNFKKSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILIGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            2360..3526
                     /locus_tag="OUB02_003262"
     CDS             2360..3526
                     /locus_tag="OUB02_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8886991.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            3551..4993
                     /gene="nhaC"
                     /locus_tag="OUB02_003263"
     CDS             3551..4993
                     /gene="nhaC"
                     /locus_tag="OUB02_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCT8886992.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYANKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFVVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            4996..5265
                     /locus_tag="OUB02_003264"
     CDS             4996..5265
                     /locus_tag="OUB02_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886993.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5341..6768)
                     /locus_tag="OUB02_003265"
     CDS             complement(5341..6768)
                     /locus_tag="OUB02_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HCT8886994.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNDLNSQTEKELK
                     RLGVKKVYIIGLSSSISNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
BASE COUNT         2507 a          945 c         1007 g         2456 t
ORIGIN      
        1 tatgagaggt taaaatgaaa caaatggaat tagctattgt aagtttaaaa aaagacgctg
       61 gagaaattta tgaaaatcaa ataaggcaat tcttaggtga taatctaaag ataaatttat
      121 actcatttga agagggaaat ttaaagtttt ttaaagaaaa acttatactc ctatctgctt
      181 atttaaaata tgatgaaata gtaaaattat ctcactatga tgctcaaatc attgttccca
      241 agttgacatt tgaaaagaac agtattgata tgatatccaa attagaaaaa gataaaatta
      301 tttatgtata taacttaagt aaagatatgg caatagaaac aatttcttta atacatagat
      361 taggaataga caatataaat atcctgccct gctatccaga gattgagttt actcccacag
      421 atgctgtaat cctaactcct ggcgaaaaaa tcttgcctaa gtttaaaaat tgtgaagtta
      481 tagatttaaa atacagaata atagatttaa gttgtattgt tgaaattgct acaaaaacca
      541 aattaaaaca tctaataaaa gatgacctaa taaaaaaata cgtagaaaaa ataattccta
      601 caagttatag tacaggtgag ttattgttag atgcaaataa gtttgaaaga caatttgatt
      661 tgttactatc tataattgat gatggaatta tatgcacaaa taacgatgga ataattcaat
      721 tctataatca tatggctaga aaaattttgt ctataaatgc aaatgaaatg attgactcat
      781 ttgttggtga ttgcataaaa gacatcaatt ttcaaagcat attgaccaat aaaactccat
      841 tttttgagaa attgattaaa ataaatcata ttgatataaa tttagagatt aaacatatac
      901 aattaaatgt atttgatgga tttatactaa agatgacaaa gttttctcaa ttagaaaaaa
      961 agcaagctaa attaagagca caactagtaa actctggtaa tatcagtaaa tacacatttg
     1021 atgacatatt aggttctagt atacaaacta ttaatacaaa aaaaattgct aataaaatgg
     1081 ctcaatcaaa ttcatctatc cttataattg gtgaaagtgg tactggtaaa gaattatttg
     1141 ctcaatccat tcatagtgct tctaggagaa aagatggtcc ttttgtcgca gtaaattgta
     1201 gtacttttca agaaaatcta ctgcaaagtg aattatttgg ctatgatgaa ggggctttta
     1261 ctggtgctaa aaaaggtggg aaaattggtt tatttgaatt agccaacaat ggaactatat
     1321 ttttagacga aattggagaa atggatttaa attctcaatc taaattactt agagtcatac
     1381 aagaaaaaca agtacgacgt attggttcta ataatgtcat tgacattgat gtaagaataa
     1441 ttgcagctac taatagaaat ttaaaagaac ttgtatctaa aaatatgttt agaagagatt
     1501 tatattttag gttgaatgtt cttcccctta aaatacatcc acttagggaa agacctgctg
     1561 atatatttga aatttttggt tcattaaaat atgatattcc atgtaatttt attctaagtg
     1621 aggaagtaaa agaaatcttt aaaatgtata gatgggaagg aaatgtaaga gaattaagaa
     1681 acttaggaga atacttctgt tacttggaaa aagatattat agaaatatgt gatttacctg
     1741 aatatatatt ggatactata gattcaaatt atagtagaac cgtttgtaat aaagtaagtg
     1801 ataatgtaaa aaagtatcaa ttcaacattg gcaaagacaa aaacatcatg gaatacgact
     1861 ataattttaa aaagtcacta gatgaataca tatttatatt ggataattta aaaaaggctt
     1921 atgatttaaa agaaagaata ggtagaaaaa gcttatgtaa aatagcttta gaggaaaata
     1981 gatttttgac agaacaacag attagaaata tgcttttaga actacaagac tttgggcttg
     2041 tagacatact tattggtcgt ggaggttcaa ttataacatc aaaaggagta gaatttctaa
     2101 aaaatataaa taggtctaac aagttaaaca gttaaaccta tttaacctat ttaattacat
     2161 tttcatatat ttaaaaattt attagaaata accttttcca caaagtttta agtataatta
     2221 atatttttat cttatctatg ctctgaaatt acttaatatt aaatattaac ttgtgaataa
     2281 atatttttta ttatcaatat tggcacgttg tttgcttaat attattaaca taaaattaaa
     2341 attactggag gtatttacaa tgaattataa ttttaatgag attgttgata gaagcaataa
     2401 tttttcatct aaatggtcag aaatggaaaa aaagtatgga actaatgatt tacttcctat
     2461 gtgggtggct gacatggatt ttaaggctgc accttgtata attgattcct taaaaaatag
     2521 actagaacaa gagatttatg gctacacaac aagaccagat tcttacaatg aatctatagt
     2581 aaactggcta gataggagac ataactggaa aataaaaagt gagtggttaa tttatagtcc
     2641 aggtgttatc ccagctataa gtcttttaat aaatgaactc acgaaagcaa atgataaaat
     2701 aatgatacaa gaaccagttt acagcccttt taatagtgtt gtaaaaaata acaatagaga
     2761 actaattata agcccattac aaaaactaga aaacggaaac tacataatgg attatgaaga
     2821 cattgaaaac aagattaaag atgtaaaatt attcatactt tgtaatcctc ataatccagt
     2881 tggtagagta tggacaaaag atgaattaaa aaaactagga gatatttgtc ttaagcataa
     2941 tgtaaaaatc atatctgatg aaatacatag tgacattata ttaaaaaaac ataagcatat
     3001 tcctatggca tctatttcta aagaatttga aaaaaataca attacttgta tggctccaac
     3061 taaaactttt aatatagctg gtcttcaatc atcatatgta atactacctg atgaaaaaga
     3121 ctataaattg ttagatgatg cctttaccag aattgatata aagagaaata attgctttag
     3181 cttagttgca actgaagctt catataataa tggtgaatct tggctagaat catttttaga
     3241 atatttagaa tctaatatag attttgctat aaaatatata aatgaaaata tgccaaaact
     3301 taaagttaga aaaccagaag gtacttattt attatgggta gactttagtg cacttggatt
     3361 aagtgatgag gaactagaaa gtatacttgt acaaaaaggt aaagttgctc taaatcaagg
     3421 aaacagcttt ggcattggag gaagtgggta tcaaagaata aacttagctt gtccaagaag
     3481 tatgcttgaa gaagctctta taagaataaa aaatgcaatt aactaaaaat atttaaggag
     3541 aatttgcact atgaaaatga atacagaaag aaaaatacct tcatttagat attccttgtt
     3601 agtaatgctt gcaataatag ctctaacatc agttggtata gttgtattta atgcttctat
     3661 aactacaatg tttttacttt catggcttat agtagttcct gcagccatga aacttggtta
     3721 tactaatgat gaaatagaag cttttggatt tgaagttggt aaagatgcct ttcaatcaaa
     3781 cttaattata ctttctgttg gtgtactaat agcagcttgg atagctgctg gtacaatacc
     3841 aacagtagta tatagtggac taacaataat aacccctaaa tattttctgc ttacaacatt
     3901 aatcgtatgt tcattaactt ctgttgctac tggtacatct tgggggactc ttggaacttc
     3961 tggtatagca atgatgagta ttggtactag tatgggtatt cctcttggtt taacagctgg
     4021 tgcagtcatt tctggtgctt tctttggaga taagatgtca cctctatcag attctacaaa
     4081 cttagctgca gcagtttgta agacagatgt aattacacat atgaagcata tgttttatac
     4141 cacaggacct gcatacgtaa tatgtatagt tctgtataca gtaattggat ttaagtattc
     4201 aaataatact atagactatg ttcaaataaa tcaaatcaag gatgtattaa attcaaactt
     4261 tcatattggt ttagtagcaa tgattccaat aatattttta ttattacttt tattgctaca
     4321 aaagccaccc attatatcta tactaagttc agctatcatg ggacttatta tagcagtatt
     4381 tcaagaagga gaaaaaatag gtgatgtttt aaattacatg ctatcaggat ttactataga
     4441 tactggattt gtctatgcca acaaactatt aaatcgtggt ggaataatga gtatggctga
     4501 aaccgtttta ctagtatttg tagttttcgt tattgctggt attctccaaa aaactggatt
     4561 cttagaagta ctgcttcaac ctcttataac taaaatagga aaatcaagaa ctaaattggt
     4621 tgggtctact tttgtagtaa gctactttgc aaatgcattt agttcatcaa tgatgttcac
     4681 ttctgtattt gtcggaactc taatgtctcc aatatacaaa gaatttaaat taaaaccaca
     4741 aaatctatct agaattatag aagatacagc tacacttggt ggtccattaa taccatggaa
     4801 ttcaaatgct gttttctgtg ctcagaccct tggtgtaagc ccactagaat ttattccata
     4861 ttgtttctta agttggatta cacctataat ttcatttatt tatggtgtaa ctggttttac
     4921 tatgcttaca tacacagatg atgaaattga agagttagaa atatttgata ctgctcagag
     4981 aagagttgat tgaatatgaa atttgaatac tattacttaa ttcaggatat tgcaggtata
     5041 ttattggcat ttataggact tagaatgtct attattggat ttaggatact ttctatgaag
     5101 gggctttcta taaatacact tttaattgta attaaatact gcttatttac catagcagga
     5161 ctgaatttat taatatctaa gtttggaatt agacactgga tttggagtgt ttgtatgttg
     5221 ataatatcca taataataaa tcccaggatt aaggtatcaa aatagaaata gttattagac
     5281 ttaaattttt ataaaaaaac tgctttacaa attaaagcag ttttttattg ttacttataa
     5341 ttaaataact ggtgtatcag ataagtaatc attacttaca taaccatatc caccattata
     5401 ctttattttt acccatccag tagcaaacat ttctattgtt tctactttgc ttccatatga
     5461 aaggtgacct atcttttcat cttctgtact tggtcctttc ctaacattta gaccatcttt
     5521 ttcttttaca tactttattt ttatattttc attactatta tcaggcttat tatttgtcaa
     5581 ataagaccca ctaacatatc caataccttc attgtatttt atttttaccc atccagtttt
     5641 taacacatct aaaacttcta cttcatcacc attatttaga tatcctataa cagaagcttc
     5701 tattgtagca tcatttctta tatttacctt atctgtatta gttacataca ttactctctt
     5761 attgttacta tttgcctgta aatcttttaa ttctttaaat gcattttcat ttcctccact
     5821 accaacttga gttatagagg tagcattttt attcgaaaat acactctttt gtgttgcact
     5881 taattgttct cctacgatta gtacaggaga attttctttt gatgctagta ctcctactgc
     5941 tagagcgtct ataagttgat tttgattttt cattccatcc ttagccacaa atacattgtt
     6001 taatttatta gatgtataaa acttttctat tactttacca ttagtttcat ttctatctgc
     6061 tccacctatt ctttctgaat taggtaaact ttgttttatt ttatctgata cagcattagt
     6121 agagcctatt atgtatgatt tatttatatt cttagattct ataaaatctt taaagctaga
     6181 tataccagat gcatcagata ctggaagtat aaccattcca ttagtagcag ctacagaagc
     6241 tatactaact gcatctgcaa gacctgtgta cccatttact acagctattt ctgatatatc
     6301 ttttaatcct tctatttttt tagctatact caaagctgtt tggtatctat cactaccacc
     6361 aactctatca gtagttaaat tattagattc taactgtttt tgcacatcat ttgatataga
     6421 actagataga ccaattatat aaactttttt aacgcctaat ctttttaatt ctttctcagt
     6481 ttgtgaattt agatcatttt tttctgtcaa taatatagga gcatttttta atttagcaaa
     6541 tggtgttaca gctaatgcat ctacaataga ttcactattt acaactacag cctcatttgc
     6601 actactccat ccttttttac ttacttcaac agcagtttga tatctatcct gacctactaa
     6661 agtattctca actgttgttc tacttatttc atttgcatat gctactggtg ataagcttaa
     6721 aactagagat aagcacagca ttgatgatat tgatttagtt aatttcatct tgactccccc
     6781 taaaattata gtatctaaat gtttcctatt taaaatagta taacataata atttaagtaa
     6841 catcattatt ataaaatatt atttttctta tctaataagc ttttattctt acttgacatt
     6901 aatactttgc tcatc
//
LOCUS       DAKBNN010000199         6889 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000199 DAKBNN010000000
VERSION     DAKBNN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.204"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            86..226
                     /locus_tag="OUB02_003266"
     CDS             86..226
                     /locus_tag="OUB02_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886995.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(223..594)
                     /locus_tag="OUB02_003267"
     CDS             complement(223..594)
                     /locus_tag="OUB02_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8886996.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1143..1400
                     /locus_tag="OUB02_003268"
     CDS             1143..1400
                     /locus_tag="OUB02_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886997.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1510..2190)
                     /locus_tag="OUB02_003269"
     CDS             complement(1510..2190)
                     /locus_tag="OUB02_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCT8886998.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2449..2748
                     /locus_tag="OUB02_003270"
     CDS             2449..2748
                     /locus_tag="OUB02_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8886999.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            3089..4744
                     /gene="ilvD"
                     /locus_tag="OUB02_003271"
     CDS             3089..4744
                     /gene="ilvD"
                     /locus_tag="OUB02_003271"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCT8887000.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGIVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIRENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            5088..5717
                     /locus_tag="OUB02_003272"
     CDS             5088..5717
                     /locus_tag="OUB02_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8887001.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEYLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            5743..6555
                     /locus_tag="OUB02_003273"
     CDS             5743..6555
                     /locus_tag="OUB02_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8887002.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2716 a          767 c         1079 g         2327 t
ORIGIN      
        1 aatacaatta cataaacttt attaaaaatt tgttgggaat ttctgactaa cattaagaac
       61 gtaagtttgt tgggggatgg tatatgtgga aaagtcaaac actactgtta caaaaaaaga
      121 aaaaattgaa gatataggaa tgaaattaga acatttattg agtgaagata ttacttttta
      181 tgaattatta cttgagattt tgtataaaga aaaagaagag aattaattct cttctttaag
      241 gaatcttttt aaataggcat tatcaatcat tttttctata gcttctctat cttctttttc
      301 aagagaatat attttttcta agagcttttt tgttttttca cttactgata agttctctat
      361 gattaattta tcttctttgc ttatttttgt attatcattt acgcctaata aataatctgt
      421 tgatgtttct aatgcttttg ctaatctaac tattatttca cttttaggtt ctcttaaatt
      481 attttcataa cgtgacaagc tagcctcagt tataccgact ttagtagcaa gttctttttg
      541 atttatatct aattcttttc gtagatttgc tattctatct cctaaacttt ccatatgtac
      601 atcactcctt accgaaatta taagttattt tgataaaaag taaaataaaa ttaccaaaaa
      661 tataaaaaaa aaaggttaaa ataataaaat atattgaaaa ttaccatttg tatatgtata
      721 atatgaatta tacaaatggt aatttgatga gaggtggtat gttgaaataa gaaactgaat
      781 taattaataa atatatagaa agttttgaaa acccttaatt gagatataaa caatatgtac
      841 aaaatttttt gcaagtaaaa ttaccaaatg tataaaataa atcagtagca tagataattt
      901 tatgctttaa tacattagat tatctaattt agttataatg gtcaaaaatg caatctacaa
      961 ataaaacaaa gtattatcaa aaaagtattc tttaagagaa ttttttatta gagatgtaat
     1021 tgatataaat aatcgtatta aaaaataaaa tactaagttt aaaaatttaa ataatctata
     1081 aatcaattat aaaatattat ttcttagaaa ttaaaatgaa ttaagtaaat ggaggaattg
     1141 atatgataat aattagaagt caagataaaa ctaatttaat gcagataaac caaattaaaa
     1201 tagatggtag tcaagtttat gcagtatttg aaagtaaaat tgatactgta aaaataggag
     1261 attatgaaaa taatacaaga gctatacaag tgttggacaa tatacaaaac ttcattgaaa
     1321 atggaacaaa atatgattat ataacatcaa ataaagtcag atacaatgtt aataaaatat
     1381 ttcagatgcc agctaaatag tgagtgtgca ttttaattta ataactaaag cccactttct
     1441 tttgtagtat gaaagagggc tttacatatt tgtaaatatc gaagtacact tgttttatca
     1501 ataaattatt taataaggta taatggacca attaaacaat aaaaattctc ctaaaacagc
     1561 aagtattaaa tatataatag cttcattttt gtaaagagag gtaatttttt cgcttatgtc
     1621 atcatcaatt gtaatagcaa tttcatcttg aataatattt ttatatatag atgggaaaat
     1681 atcttttttc tttataagat taaataaatt atttacacct acccatatca aatatcctaa
     1741 aacagcacct aatgtgttca tcattaaatc atcaatatct gtagctctta agttaaacaa
     1801 ttgacaaagt tcaattgcta atgaaaaacc taatccagtt aatgaaacat tgataatatt
     1861 tctaaaattt ttccaaatta aaggtagtaa aaatcctaaa ggcataaaca taattatatt
     1921 taaaatatat gttaagatgc caactgagtc aaaaggtatt agatttattt catcaattct
     1981 aaaaagccca ctataccttc ctatatccca tatgctacca attccagctt tactaagtgc
     2041 tagatagata tataataaaa aaatatatac ccatactaaa tgtatcatta cattcttttc
     2101 aactttcaaa ttttttcgtt ttataacgac tatttggtat attaagcatg gtaatataac
     2161 gcaaataaat gaataaaatg tccatatcat tttattttgt cccctttcaa atttatgttt
     2221 ttagttttat aagtactatc tttatattaa actgtaagtt ttaaaaatct tttaataaaa
     2281 tcttaaaaat ttattaattt ataaaaatat attattaaga ttaaaaatat atataatcaa
     2341 agtttctaca attatatagt taggtatata aaaattttat cgtatgtata aatattataa
     2401 atatatggaa ataataatag tatattttgg taaagtagga ggaattttat gaaatggatt
     2461 aaatggtata gtattacatg catatgtatt tttatagtag tagcttttta tatgtttata
     2521 tttcctaata aaatagaaac tattgatact tcatctgcat atagctttgt tgaaaagaaa
     2581 gtaccaaata gtgctgtgta tcaaggatac aaaaagaatc cagttgatgg tactactaca
     2641 atttattaca gctatgataa ttcaactcat atagttagat tatcacatcc agaagactat
     2701 tcaaggaaga ttaattggga taaagtttca aacatcagat ttgattaatc tttaatattt
     2761 ataaaagtct atatatctaa ggcaaatata ttactaaggc aaatatatta ttaatcttat
     2821 tcatagttga gcactttcta ttatagaagt gcttcttctg atttatatca taaaatatta
     2881 ctcaacaatt tgtaaaatat attagtttat aaaatatatt taaaagataa tattatataa
     2941 tacgattgtg taagatattg ataaaaaatc gtaataaatt ttcattagta tatgctaata
     3001 tttttttaca attttcgtaa ttaattaata gaaaaatata ttataataat attatgaaaa
     3061 tttaaaaaag taagaaagag gtaatattat gagaagtgat ataaaaaaag gaatagaagg
     3121 agctccaaag agagcgttaa tgtatggaat gggtcttact aaagaagaaa ttgaaagacc
     3181 attaataggt atagtaaatg cacaaaatga ggtgattcct ggtcatctgc atcttgatga
     3241 aatagcagaa gctgcaaaaa atggagtaag aatgagtggt ggtttgccat tagaatttcc
     3301 tgctataggg gtatgtgatg gtatagctat ggggcatgta ggaatgaatt attccctggc
     3361 ttcaagagaa ttaatagcag attcaataga agctatggct atggcacatg gttttgatgc
     3421 tttggtactt atacctaatt gtgacaagat agtgccaggt atgttaatgg ctgctgcaag
     3481 acttaatata ccaagtattg ttgtgagtgg agggcctatg cttccaggta aaaaaaatgg
     3541 aaaagtatat gattttaact ctgcaatgga gggtgttgga gcatgtaaag atggaatagt
     3601 aagtgaagaa gaattagaag cgttagccat gaactcttgt cctggatgtg gttcatgttc
     3661 tggtcttttc acagcaaata gcatgaattg tttaacagaa gctttaggca tggggatacc
     3721 atataatggt actgctgcgt cacattctgg agaaagaaaa aggatagcaa aatatgcagg
     3781 tatgtatgtt atggagttac ttaagaacga cataaaacct agagatattt taacaataga
     3841 tgcttttaaa aatgctatag ctgtggatat ggcaatggct ggttctacaa atacagtact
     3901 tcacttacct gcaatagctt atgaatcagg aatagagctt aacttagatt tttttgatga
     3961 aataagtgaa aaaactcctt gtttaacaaa attaagtcca agtggaaaac atcatattga
     4021 agatttacat atggcaggag gaataccagc tataatgaac gagctttcaa agataaatgg
     4081 aataaattta gattgcaaaa ccgtaacagg caagactata agggaaaata taagaaattg
     4141 tgaaatagaa aatgaagaag taatacatac attaaagaat ccatatagta accaaggtgg
     4201 gcttgcaata ttgaaaggaa atcttgctct aaatggagct gttgtaaaaa agtcagcagt
     4261 tgcagaagaa atgttagttc atgaaggacc agcaagagtt tttaattcag aagaagaagc
     4321 tgtaaatgct atttttggta aaaaaataaa caaaggtgat gttatagtta taagatatga
     4381 aggtccaaag ggtggtccag gaatgaaaga aatgctatct cctacatcag cagttgcagg
     4441 aatgggactt gataagcatg tagcacttct tactgatggt cgtttttcag gggcaactag
     4501 aggagcatct ataggccata tttctccaga agctatggaa ggtggtttaa tcggactagt
     4561 tgaagaagga gatataattt ctataaacat accagacaaa aaattagagt taaaagtaga
     4621 tgaagttgaa atagaaaata gaaaattaaa atttaaacct ttagaaccga aaataaagca
     4681 tggatactta agtagatatg ctaaattggt aacatcagca aatacaggag cagttttaaa
     4741 atagtagact atatttatgt aaattagaaa aagttaagaa aatattaatt ggatatctta
     4801 ggtgttatat cactaatgag gtatcctttt tattttgtat gaaaaacaca gcaaaatctt
     4861 gattatcttt gaaaacaata catctctatt ataaggaata cgagtacagc atagagatgt
     4921 atcttttaga ccttgttgag gtaatacaac tttttaaaag tttgatgtgg aatgcttttc
     4981 attccatctg aactatgaag aaattgaaag tcagttaatt cttgacataa taaatattaa
     5041 aatgtataat aaaaaacaaa cagacagtct gtttttagga gggaaatatg aatagagaag
     5101 aaaaaagtaa aaatagtaaa gaaaaaatta tccaatcagc attttcacta ttttcgtcta
     5161 aaggatatga ttcaacatct acacaagata ttatcaattt atctggtcta tctagaggtg
     5221 caatgtatca tcactttaaa actaaagaag atatactgag aagtgtcaca aaagaacttt
     5281 actcacaaat gaataatttt ttagagtatc ttgttgctga tgacaccctt acagcaaatg
     5341 aaaaaataat agaattggtt gttcatagtg cgaatgatta cacacgtaga aaaatggtac
     5401 attgtagctg gttagaaaaa atcccattcg ctttaataga ggaagttcgt aatctcaaca
     5461 atgtagttgc acccaatatt gctaagataa ttaaacaagg tgttgaaaat aaagaatttt
     5521 cttgtgaata tccagaggaa ttagctgaaa tgctcgtttt tagtattgat attttacttg
     5581 accctgtatt atttaaacgt gaatacagtg aagtatgtaa taggttggat tttttattat
     5641 tcatgttaaa aaaaatggat attcctctaa ttgatgagta cggtattcaa aaatttaaag
     5701 atttatttaa acaatgataa taggaaaagt gaggaataga ctatgtttta taatgcaaaa
     5761 agctgtcaaa tgatgatagg aagtaataca gtgaattata ttgaatttgg tagtggtaag
     5821 aagacactaa ttattttacc aggattagga gatggtttat ttcctttaca tggaaaaata
     5881 caggcgattg cttttgcttt tagatataag caatttgcaa aagattataa ggtttatgta
     5941 tttagtagaa aaaatcaaat tacagaaaaa tattccacaa gggatatggc taaagaccaa
     6001 gcagatataa tgaaaaaact tggaattatg aaagcagaag taatgggtgt ttctcaaggt
     6061 ggaatgatag ctcaatatct ggcaattgat tatcctgagt tagttgagaa acttgtgttg
     6121 gcagttactt cttcaaagca aaatgatact atccaaaatg ttatttgtag ctggattgat
     6181 atggcgaaga agcagaatta caatgattta atgattgaca cagcaaagaa atcctattct
     6241 gaaagatatt taaaaaaata ccaattattt attccttttt taggaaaagt aggtaaacca
     6301 aaggatttta aacgatttat tattcaagcg acctcttgca tagaacataa tgctttttcc
     6361 gaattaaata aaataacatg tccaacctta attataggag gtgctaatga taaaattgtt
     6421 ggaaataatg cttcttttca tttagcagaa aaaattaaaa aaagcgaaat atttatctat
     6481 gagggattgg gacacgctac atatgaagaa gcacaggatt ttaatgaaag agttttagag
     6541 tttttaaata agtaaacacc tttgttaaaa atatacatat gaagattgga aatttaatgt
     6601 taaaaataga aacatgaaaa tatgcttaac tggtattttt actattcata accaattttt
     6661 gatacattat ctactataaa tacaaatata gcttcaatgt gattatatat tgttgtattg
     6721 gtaaagcact tatacaaaca gaggaatttt gtaaattcat attataccca catttgttaa
     6781 cttatgaaaa tataatcaaa atttttatga gctttttata taaaaaatat aatgtttgta
     6841 tacaaaaata ttaaagacat tttagtaagt tttgtatata agcaaacat
//
LOCUS       DAKBNN010000200         6849 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000200 DAKBNN010000000
VERSION     DAKBNN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.205"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(125..1498)
                     /locus_tag="OUB02_003274"
     CDS             complement(125..1498)
                     /locus_tag="OUB02_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887003.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISASAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(1664..2986)
                     /locus_tag="OUB02_003275"
     CDS             complement(1664..2986)
                     /locus_tag="OUB02_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8887004.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(2979..3683)
                     /locus_tag="OUB02_003276"
     CDS             complement(2979..3683)
                     /locus_tag="OUB02_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887005.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(3866..4930)
                     /locus_tag="OUB02_003277"
     CDS             complement(3866..4930)
                     /locus_tag="OUB02_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCT8887006.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(4933..5631)
                     /locus_tag="OUB02_003278"
     CDS             complement(4933..5631)
                     /locus_tag="OUB02_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8887007.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(5622..6719)
                     /locus_tag="OUB02_003279"
     CDS             complement(5622..6719)
                     /locus_tag="OUB02_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCT8887008.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
BASE COUNT         2363 a         1201 c          724 g         2561 t
ORIGIN      
        1 taaagaatac taataaaaat atcaataaaa atagatgtat ggcttagaat tagtaaaaca
       61 attataataa attatagcta cacatctatt tttaatttat acaattaaag atttattcga
      121 tttattaaat aataaacatt gctactatgg ttgttacaac caatccggtt ataactggga
      181 caaagtttcg ctttgccaat tctattggac taactccaca aattgccgat gctgaaataa
      241 gaccccaagg aactagacag cctccaccta cccatatacc actgatttgt cctaatgcag
      301 ctagtgcacc aacacttgca tttatagcag ttccaaatac agaggcaagt gaaccaacta
      361 atgaaatccc agaaaatcca gaaccatcaa gcccagttat tgctcccact acagtttcta
      421 tacctgatac ggcaaattta ttaagtggta ttgtttgaga aagagctatg cctatatctg
      481 ataataaacc ttgtgaacct ggtgctaata cttttccaaa tacacctgtc aatggtgcaa
      541 cttcacccat atagaaaaat gctgctattg gtataattgc tccaaatatt tttatcccaa
      601 acacaaaacc atcaattata ttctcacata cattttcaag actatctttg ccaaaattta
      661 taacatttat aattattatt aaaaatactg ctgttccacc caaaagagct gatgcatctc
      721 ctccttttag gtcaaataca tacatagcaa ttatatcagc aagaaatccc aaagctacta
      781 aaatagttgc tattttagat tttccattaa actcttctac ttcttcactt tcaaaacctt
      841 ttagttcttc tctaaacatt cctttgttaa tatctctttt taatgtataa aatgccatag
      901 atatggttac gattcccatc acccaaaaca aaatcatacc atcatttata acatatgata
      961 ctgcaacacc tgctgcacca gctgttatac ttggagctcc ttgaataaca aagtctgtag
     1021 ataaggcaag accatgacca aataaattta atgctactgc aacaccaatt gctggtagcc
     1081 cagctctcat agcaacaggt aaaaatacag ctccaactaa ggcaacagct ggagttggcc
     1141 agaaaaacca agaaagaact aacataacaa atccagttac aaaaaatgct gtattagaat
     1201 tttttataac tctcgaaaat ggtctcacca tatactgtat agcattattg gcttctaggg
     1261 ctttagataa tgcaaccata atagctataa tcaagataat tggacccaac tcatttaaag
     1321 atataataaa agagttaaac actgctgtta tagcaccaaa aatgtttttt tcaaagaaca
     1381 accccataaa aaaaacacct aatatacatg gtactattgt atcctttttc ataatcatag
     1441 taattaatat aattaccata aatagaatgt aaatataatg tgctcctgtc aaaaccatat
     1501 tattatgtcc ccttttttta agataataat attatatggt cttgtaagta aacttgttac
     1561 ttgcctagag gtacacaata ttgaacatcc cataactaaa gtcacatgct tcctgcttca
     1621 tagaatttta cataagcaat tacacaatat ataatttaaa tttttatgat tcttttgctg
     1681 gaatagatat ttcaatagca aattctttat catctgaatt tacatttatg cttccattat
     1741 gatatgcaac tattttctca caattcttta taccaattcc aaaactttct acattcttag
     1801 aaaaattact tactctattt ttttgtataa ttagtaattt agaatcctct actttaatta
     1861 aaaatactat aggcttatct ttcctagcat attttattat attagaacat aaattatcaa
     1921 atatccttct aaattgtgta acatctacat aaatatgata ttggttataa agttcatttt
     1981 ctattgttat atgaaatcct ttctcctcca atgtatatga actatctaaa agtaattgaa
     2041 aaataaattc attagcattt attttttcat acccttttga aacttcctct ccagaattaa
     2101 caaaatgcgt aaaaaggtca tctgttaaat cttttatttg atatgcttta gaccttacat
     2161 tttttatgta ttgttccaat tgctcaggac tattgtattt atggtcttta atcaaatcta
     2221 aataacaaat tactgacgtt aatggagttc taacatcatg tgatatcgaa gttactatat
     2281 ctttattttt attcttcatc tccctttctt ttataagttg attctccaat gtatgactca
     2341 tctcattaat actagatgca agtgatgata attcatcatt accttttact gggattctat
     2401 actttaaact gccactctct actatcttta tacccatttc tattttcctt aggtacttta
     2461 tctttctact aaacaacagt aaaaatgtta taataaatgt aagtgccata aatgtaatag
     2521 aaattgcttc tataaaattt tgaaatctaa atctaaataa agctactaca actatattag
     2581 ctttaatatc agaaaattgt atctcatagt ttctagccat gggactgcta taaaactcat
     2641 tttcctcact cttatcaggt acttcattag gatagtataa tgtatcataa aaaaccttat
     2701 ttcctttata tatcttcata gaaacaaacc aattatcact attccatttt gctatatcat
     2761 ctccatcatc tgctcttata ccattgttat tcacataagt ttgaaaatta tccataagtt
     2821 gatttacttc tcttttaaaa tatactttcc cttctgatac atcttttact atagattcaa
     2881 cagcagagta aaatggtata tttaaaatga aagccactgc agctgagatt actacatata
     2941 tcaatagtat aactcccaac ttagatggct taattttgtt aatcaatata ataccccttt
     3001 ccccatgcgg tttttatata ttgtggattt ttttggtctt tttctaactt ttttcttaaa
     3061 tttctaatat gaaccattat tgtattattt gctgtataaa aatactgctc atcccaaata
     3121 gtttcatata aattttcaga cgaaaaaact ttttttctat ttttcaacat aagaattaat
     3181 atactatatt ctatagttgt caaaaacaca ggttctccat ttagtgtaac atcttgagta
     3241 tctaaattga cacataactc ttttgttttt aatatatttg gctctctttc cttttccttt
     3301 ccctggtata tataataacg tctaagtaga gacttaaccc ttgaaattag ttcagaatat
     3361 gaaaaaggct ttgataaata gtcatctcct cctgctgaaa atccaataac tttatcagag
     3421 tcttgagttt ttgcagttaa aaataatatt ggtgctgtcg ttttttctct aatttcatta
     3481 catgcaacaa atcctgtttt tacaggcatt acaacatcca aaataatcaa atcaatcgta
     3541 ctatctacta agtttacagc ttcttctcca ttcgttgcca taataacttc ataaccttca
     3601 ccagttaaaa gtatttctac gatttcccta atctcaggat tatcatcagc tactaaaatt
     3661 ttttttggaa tctctttatt catcaccaag ctcctttttt tgctatattt cttaaatatt
     3721 tcaaactata taaaatatta tactttatta gcaaaagctt agcaatgtca cacgttttaa
     3781 tagtttctac tcctgtaaac atgtttaaag cttaattaaa atatacacac aaattgacat
     3841 attaataaaa ataatctata taatattatc ttacataagc aacttttttc actgctttta
     3901 ctttttcaga agattttaca tgtactacta tacatatcaa tccacttata gctacaaatg
     3961 cataaaaact tatcccccta ctaagtaaca ttgccggaac taataagtga ccagggaaaa
     4021 atatcttaaa taatgtcata aaactatttt cagaagctcc aacagcccct ggcaatggta
     4081 atgaagaaac agcaatatta agcaaggatt gtatcgctaa tatctcaaat acagtatatg
     4141 ttgatagatg aaaagctttg tatacaaaga aaggaactaa aaacatagct gttatttgta
     4201 caatagttat gactaaaatt tggactacaa gttttggatt ctgctttata tattgagcac
     4261 ctttaacata ctcttcaatt tgagatatta taacttttct tgaactttca acatctttta
     4321 ttattcttaa cttacctaat aaatttgtta ttgatattat aaaattgttt acaagttttt
     4381 ttgaaaatat aattgcaatt actccaatga caagagcaac atttgttatt acaccatata
     4441 ttagtaaaat gttcatacca cttacattat ttaatataaa ctcacgttcc ataatgaaca
     4501 ttattccact atatgccagt ataactactt ggtgaataac aacaatgact aatagtataa
     4561 gtgaagaata tgatatattt atatcgtctt ttttcatata ataaacttgt gctggttgac
     4621 cacctgatgc tgaaggtgta atagaactaa aataaaatcc aataaaagca tatcccatac
     4681 attttaaaag agtagtcttt tggtttaaag ctttcatacc taagtatata tttatacctt
     4741 cacaggcaac atatataaac atcattgcaa atccaattag tataaacata gggcttgcac
     4801 cttttaatga ttttatcaat gattcaactg actgccctct aaaaacaaaa tatcctgtta
     4861 tgcccattag taaaactaaa aaagctacac tacctaggca tttatttttc ttttcagttt
     4921 tacttatcat aactacatcc tttctggtgt tacgaacttt aaaccactgg ctttaagttt
     4981 tggaatagct atctttaaag cttcaatagt acgctcaggt gcacctactg caccacctga
     5041 attttcacct gcatcatgta aacaaattat agaattttct tttgttctac tcattaactt
     5101 gtttataata atgtctactg tactatcttt ctcccaatct tctgccatta catcccacaa
     5161 agtcattttt agattatatt ttttaacaaa tgaatttgaa aatatattag tatgacccca
     5221 tggtggacga tagtagttta catctacacc taaatttttc attatatttg tactttcaat
     5281 aaaatctttt ttaacataac tataactata aagccatgca tttctgtgtt ccaatgaatg
     5341 taaaccaact atatgatttt ctctcaacat tctttttata atttcaggct ctttttcagc
     5401 attctttgct actacaaaaa acatagcttg tatatcattt tcttttaaca catctagtaa
     5461 tttttctgta tatctcttat ctggaccatc atcaaatgta agtgcaattt cattttctcc
     5521 agccatattt tttaatacct ctttatttaa tagtttatta tagtatgtag gtataattga
     5581 atggactaaa aaaatcaatg caacaacgat aattaaacct actacataca ttctctacac
     5641 gctccttgtt catcaagtga aaataaaata ttctctaagg tattattatc aaattgtttt
     5701 tttaattctt tcatgttact gctcatttct ttaagagtag catcatcata aattaaatct
     5761 tttatctcat ctacatagta ttttttattt ttaccaagta ttctcccaat ttcattattt
     5821 aatataaaac tagcattatc tatctcttgt tgtaaaaatg gattaaatgc caatattgga
     5881 agttctgaat aaattgtttc aaatagtgtt attccacctg gtttagaaat aataaggtca
     5941 gaatctttca tatatttata tacttcattg gtgtaaccta ctacctctat attttcatat
     6001 ctaccatata gtttatagta cattttttta ttattaccag ttattacagt agtttttact
     6061 ccttctaaac tatttaattc tttgtaaaat tgttctgatt taggcagtaa tcctaatcct
     6121 ccacccatta taagaatatt ttttttagtt gaatgattta catgttctat tcttttaaat
     6181 tcttttttta ctggaatacc attaacttta atcttagatt catctattcc tttaaaaaca
     6241 agttcttcct tagtagattt actagctact aaataacagt ctgtattttt actaatccat
     6301 tctgagtgac tacttatatc agttatacat gttattaaag gaagatttga gttatatttt
     6361 tccttatatc ttgatactaa ttgagaacaa aatggaaaag ttgaaataac tactgttggt
     6421 tgtacttcat gaagtaatgt atctaattta tttaaaaaat aatctgaaaa agtaaatttt
     6481 atatctttct ttccattttc agcacattta taaaataaat tataaagact acttccccga
     6541 tttacaagaa tagaaaaagt cttgtacatt ttatctgaat agttaggcat aatgtactca
     6601 aatatatctt taattattat ttctgaatta tcaaattttg cttttatatc ttcacttaaa
     6661 gaatttgatg ctgagtaatg acccatacca aattttcctg taagaattaa taccttcata
     6721 agattccctc ctcgttaaag cttttctttt catgtgatta ctatacttat aagtatagac
     6781 tttaaatctt tagaaaaacc tagtataatt cttaagaaat cttaaagttt taattaaaaa
     6841 atacaaaga
//
LOCUS       DAKBNN010000201         6844 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000201 DAKBNN010000000
VERSION     DAKBNN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.206"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(545..1759)
                     /locus_tag="OUB02_003280"
     CDS             complement(545..1759)
                     /locus_tag="OUB02_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCT8887009.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASYTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            2185..2901
                     /locus_tag="OUB02_003281"
     CDS             2185..2901
                     /locus_tag="OUB02_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HCT8887010.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            3036..3662
                     /locus_tag="OUB02_003282"
     CDS             3036..3662
                     /locus_tag="OUB02_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT8887011.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(3862..5406)
                     /locus_tag="OUB02_003283"
     CDS             complement(3862..5406)
                     /locus_tag="OUB02_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8887012.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(5432..6073)
                     /locus_tag="OUB02_003284"
     CDS             complement(5432..6073)
                     /locus_tag="OUB02_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCT8887013.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            6343..6534
                     /locus_tag="OUB02_003285"
                     /pseudo
     CDS             6343..6534
                     /locus_tag="OUB02_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         2518 a          877 c          973 g         2476 t
ORIGIN      
        1 cgtatttcaa ttaaattcaa ttaaaataaa taactcttta taaaaagcaa ttatttaggt
       61 agaggaatat ttgctaactt taatagtctt tgttaacttt aattttattt aagttattat
      121 agtcttacta attttataat tgtagactta actactttat gttcttttat tgtttatgta
      181 gttgatttag aagttatttt aaccatttat tttaattgaa gttgtttgat ttttaaatcc
      241 tttgtgtaat ctttgttttt ctagccatga taaaagtatt tctatttgta tcactatata
      301 taataatatc atgacaaaat atttattgtc taactaatta taatctatcc aaaatgaaaa
      361 tgggtgtgag atattattac aacaaattgt attagtatta aaaagattaa aaaattaaaa
      421 aactaaacag ttaaataatt aaacgattaa gtgactgtca tataacaatt tataatttta
      481 tttaacagcc acttaggtat ttaagagcga tagaattgag tgtatatcaa attataataa
      541 tttcctagta aataattgaa aaataaggta gtccattttt atatttgata tctggtttac
      601 cttgaataag tggatataga taatttaggg cttcatcagt tatataatta cattcttcat
      661 ttatccactc tttaggaaaa tattttgtct tgttagctat tttagaaata tcagtatgac
      721 taacttcata cttataagga ttattgcatg ttctattaat gatagccata actcctgtat
      781 atcctttttt agatagttct atcccatatg caccaacttc agctgcttgt tttaaatcag
      841 tatatgaagc taaatgcata gcacatcttt gtattacacc aagttctaag gtttttactc
      901 ttgttgtaat tgatgatgat actataagat tctttagata gttgcttact ccacctaatt
      961 gggaatgacc aaatttatca tgagcagaag ttttttgttc gcatataaag ttgccgtatt
     1021 tatcccttat accttctgat gcaactatat aagccttatt tttttcttta aatatttttc
     1081 taacatcttc tataaactta tcttcataga aatcaatttc tggaaggtat attaaatcaa
     1141 caagtggagt atcattaagt ctagctagtg aagaagaagc tgctaaccat ccagtatctc
     1201 tacccatagt ttctactata aatataccat tatttttgta tatagaaaaa tctaagtatg
     1261 tttccattat tagtgtagaa ataagctttg cagcagaacc aaatccagga cagttgtcag
     1321 ttcctaataa atcattatct attgttttag gaatacctat aaattttatt ttacaagtag
     1381 tgtttttgca gtattgagaa aatttcatca ctgtgtccat agaatcattt ccgcctatat
     1441 aaaataatgt atctatttca tattcatcta gtatatttat aaccctatca taatcataag
     1501 aatttatttt ataatcagcc agtttgtatc tacaagaacc aagaccagac gaaggtgttt
     1561 gctttaaatt taatacatta tctttgttta aggagtctga atctattata tttttgttta
     1621 atataccttc aatcccattg ataccaaaat atactttatc aaagtattta gtttctaaat
     1681 ttttttcata aataccaact gcagaagcat tgataacagc tgtagggcct ccagattggg
     1741 caataataca gtttctcatt ttacatctcc ctttcattaa tattgttatg aatggtactt
     1801 tttatcgagt aaatttatat ttatagtctg attatatatt aaatgaacta gctatatcaa
     1861 ttgagttagt atagacaata aatgccataa gaatgtggta aaaaactaac tttttatata
     1921 caattaatgt ctataattta ccacaatata aaggtacttt taaggagtag tcaaagtgtt
     1981 gtgaaaaaaa atatatcagt gtaatattaa tttacaaagc aaaataagtt cacagttaac
     2041 aaaagcagaa taagttcata aaagaaatat atacagcaat aaaagtaatg ctaaataaaa
     2101 gtaatactgg attaaaaaat attaaaaaaa tatcaaaaac taatatttaa tactaattaa
     2161 agtaaaatat ggaggtatac aaatatgaaa cacataaaaa tagcagcagg attagcacat
     2221 gtaaattacg gtcatatatc agccgtagtt aaagaagcaa tggatgcagg tgtagactac
     2281 gtacattcag atgcagcaga tatgcatgac ttaaaaaata tgcaacttat gggtggtcat
     2341 caaataatag aagcaataag accagtgaca gataaaccaa tagaatgcca tatatacaca
     2401 agagattgtg atagattatt tatagaaaaa atagcagcag caggatgtaa cttgttgata
     2461 ataccagcag aacatttttt aggtgctcct cttgcataca taataaatta ctgtagagaa
     2521 tttggtatga aagtaggatt aacaattgga tgttacacac cattatgttt tgttgatgaa
     2581 gctatatatg atatagatag attgcaaata gtcgttcatg gtgttgatga aactgatgga
     2641 aaagataact ggggatggag aaagagtgct gttgatttag taaaaagagc tagaaaaatg
     2701 atagacgaaa agaatcctaa gtgtgaatta gcaatagatg gtggactaag agcagacaat
     2761 atggagccat taatagaatg taatccagac gttgtaatat tatcttcagc aatattcaag
     2821 gataaagatg gtataacagc aggtgttaag aattgtagaa aagctataga tgaagcagct
     2881 acaaagtttg gtttagaata agataataaa ctatgataaa agagatgctt tcttaatgtt
     2941 ttaaggaagc attaatttta tgaaaaacta caggaagcta gactaattta attgattttt
     3001 tatactgaaa caatataatc ataggaggtt attaaatgaa aaaagaaatg ataaaagatt
     3061 atttactgat aacaatatca gcatttttaa tggcattagc agttaatttt ttctttgcag
     3121 aacatacatt agctccagga gggataacag gattatcagt cgtacttagc acattcttat
     3181 caatgcctgt tgagaatata tcattaggaa tatctatgcc acttttagta atgggattat
     3241 tatttttagg gaaaggtttt ggaataaaaa cattatatat aactcttatg ggcccattat
     3301 tcttaaaagt aataccacaa actcacataa ctgacaatct attagtagca ggagtgatag
     3361 gtgggttatt ggtaggaact gctataggga tagcaataat aaggggctgt agtactggtg
     3421 gaactgattt agttgcaatg cttattaata aggtaattaa gtttctgaag ttgccagtta
     3481 tattatttat gctagatgga tttataatag tagcgtctgg aataataagt aagaatttta
     3541 tgatatctat atattcattg atatctttat tactcataat aaaaacaata ggatttgtaa
     3601 ctacaaagtt tggtaagaat actgaagaaa ttaataatat aaataatgct tgtgctcaat
     3661 aaaaataata aaatttactt taaatataaa agtttaaaaa atataaaagc ttaaataaaa
     3721 agattaaata taaaaaatga aggtgtctca aaatgaattt tttagttcat gaggcacttt
     3781 ttctatgaaa tcaaatatat ttttatcatt tggacaatga aaaataggtt tatctctatg
     3841 ttgagacaag cctatttttg tttatttata agataatatt ttattttttt cagaatatag
     3901 tttttttata attgtactaa gtctagaaat atcattctta cttaaaagag catttatatt
     3961 tatcagttca gcattatgtt caaagtctac aggagcagta gttagtatta agtccacatc
     4021 atcaaagttc gtaaatgaaa tagaagatag aggtatagat ttaactatat taatctgatt
     4081 aaattcaaaa cttaactttt ggactaataa attagataca ccatcactac agtgacatat
     4141 taaaatagta tttaggggag atttgcttct atctattgct gatattaaat ataaagcaag
     4201 atatcctatt tcgtcatcat tgacttcata attcatcaac tgtttgataa cagtattaga
     4261 ctctttagca gccttataag tataaggaaa tgttctcttt atatcattta atatgggatt
     4321 tgtaatagta aacccatagt ttattctgtg tagaactgat ttaaggtgat tagcaagaga
     4381 ctttattaat attaaatcat cctttaagtt taagtttagt atacttcccc aatgaacagc
     4441 tattgcttca gatagattat actctgtaat ttcttcattt ttaagaggtt caagagtagt
     4501 agttttcttt attgatttaa gtaatccttc tatatgaaga aggatataac agacctcttc
     4561 atcaataaat tctacgtcaa aagcttcact taggttagca cataaaattt ttgatttttc
     4621 aaagtattca tgttctaata attctctaaa aaaaccttca gataagttaa tatatctttt
     4681 aattgaaatt cttttaatac atatagctat atgtattaag aagtttatat aatactcatc
     4741 actaaggtta cttttttcta cttgtaaggt gtgtagagtg atttgagata atttctcaaa
     4801 atcatagtta aatagttcct taaatatttt tgttgatggg gtatctttac attcattaga
     4861 tttcgagaat aatatttctt taagttcagt ataactttta ttaaccgtag caagttcaaa
     4921 aattgctttt cgaatatctt tctcttttcc ctctaaatat actccattat tggtttttct
     4981 aactagagtc actttaaagt tttgcaaaaa attttgaata actaccaagt ctttatgcac
     5041 agtagctcta ctaacatgta gttcattgca aatatcacga atcctaaact tagaattttt
     5101 aataaataat ttacttagaa tatacctttt tctatcttca ggagagtatg catatataca
     5161 atttgattta tcattgatat ctctaataac tttaagtttt atatcttcat tacctagaat
     5221 aataacccct gaaccagttt ttttatctag ggataaatta aactctaaaa gccattcatc
     5281 taaaataaca aggtcattac gaatagtttt attactaact tttaattctt cggcaatagt
     5341 atctacagta atatagtctg agctgttgag taataattca gcaatatcta ataatctttt
     5401 gttcaatgca aaccctccta aatcttagtt actatttatt aaccttggac caatctcttt
     5461 caaaagtatt ggcaccatct attgtgagct ggttaaaaga agctttttta aataaatcat
     5521 atgttacagc tgcataatca catccacaaa gagatgcttg agttaagtgg aatacatttt
     5581 taatagaagc tgcaacaact ttagtattat atccatttat tgatataagg ttacatattt
     5641 ctgtaaggtt ttttatacca tcattcccat tttcatcatt tctacctata aaagggaata
     5701 ggtaatcagc acctgcattt atagctaatg atgcttgatt tacattgaaa atcaaggtac
     5761 atgcagtttt tacaccttta ctatttaata tgtttataag ttctaagcca acttcagaga
     5821 aatttatttt tattatagtg tttttattta tatttaatat cttatcacat gaattaatta
     5881 attcgtataa attatttcca atgacttcag cagttaatat ttctggattt aaattagcac
     5941 atgtctcaat aaattcatgg aagtttactt tctctttaag atacatagat ggattagctg
     6001 ttattccctt aaatccatac tttaaacact tttgaatttc atcaagatta gcactgtcta
     6061 tcaaatattt cataatttaa ttctccttta agctatttgt cataaaaaag tgtatatact
     6121 ctatttttat tattaagtca attatacaat aagattgatt ataaaattat aacatgggta
     6181 cttaataaaa tttacataat gatatggtaa taataaaaca tttattgtct aataattgcc
     6241 ataagcttta gtaaataaga gttaacagtg aaggtattgc cttaaaaatc ctatgatatg
     6301 tataatttga atatgaaggc aataatttaa gggaggaaaa atatgattaa cttttatcaa
     6361 ccatcaagaa taaattttgg acaaggaact ttaagtgaat tacctagaat aataggaaaa
     6421 tatggaaaga aatgtttgtt agttactact ccaaatgtag agccattaga caagttatat
     6481 aaaagagtta cattgaattt aatagaaaaa ggtatagaag ttgtacactt tgattaaaaa
     6541 atagctacgc acactcgtga ctttagtcat gagttaatag ctaaaatagt cagcatatag
     6601 ggaaacctat atgtagagat aggatagaac ctatccaata ctacttgaat tgctggaaac
     6661 ccctaaagct aaccaaacta aaacataagg atgaaataaa cctaagtgtg aaagttacga
     6721 aagtagaaaa aattggttag atggtataag gttaaatcct aagtacttta aaatgggcaa
     6781 tcagcaggta agctccgaat aggagaaact tcaacgacta ttcctcttga gggaagtaca
     6841 ctac
//
LOCUS       DAKBNN010000202         6653 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000202 DAKBNN010000000
VERSION     DAKBNN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.207"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            153..1304
                     /locus_tag="OUB02_003286"
     CDS             153..1304
                     /locus_tag="OUB02_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887014.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNDFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1422..2060
                     /locus_tag="OUB02_003287"
     CDS             1422..2060
                     /locus_tag="OUB02_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HCT8887015.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2061..3083
                     /locus_tag="OUB02_003288"
     CDS             2061..3083
                     /locus_tag="OUB02_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCT8887016.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3073..3693
                     /locus_tag="OUB02_003289"
     CDS             3073..3693
                     /locus_tag="OUB02_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887017.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3817..4503)
                     /locus_tag="OUB02_003290"
     CDS             complement(3817..4503)
                     /locus_tag="OUB02_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCT8887018.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4517..5062)
                     /locus_tag="OUB02_003291"
     CDS             complement(4517..5062)
                     /locus_tag="OUB02_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887019.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5290..6537
                     /locus_tag="OUB02_003292"
     CDS             5290..6537
                     /locus_tag="OUB02_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCT8887020.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
BASE COUNT         2638 a          695 c         1100 g         2220 t
ORIGIN      
        1 actataaatt aggtttatat tttataaaaa tttgctaaaa aaaacaatta aaaattgtaa
       61 agaaaaaaga tataattaga actgtagtaa taaatgtttt attatagata atagagtttt
      121 aagtatctta aataatattt ggaggaatat gaatggaatt aagaccaaga agtgcaaaat
      181 tagaagaatt tgataaagta atagaattaa taaactatgt atttagaata tcaagaaatc
      241 ataaaccaac tatgatggaa gaatttcctc ttttactaag taaaaataat atagaaaata
      301 tgattataat atcagaagat gataaagtgg tttctgatgt aaactatcta attcaggatg
      361 tatctattca aggaaacaga ttgaaggttg cagctatagg tggagtatgt actcatccag
      421 attatgaaaa gagaggctat tcatctaaga tattggataa agtagaagaa aaaatgtttt
      481 atgatggtgt agatattgta attatatcag gaacaagaag tctatacagt agaagaaatt
      541 gtagtttggt aaagagtttt tataaatata caataaaacc tgaagatgta aaaatagcat
      601 atgaaatagt tgaatttgat gaaactaatt ttgaaaaaga taatgattta gataaaatga
      661 ttgaactata taatcaaaat agtactagat ttataagaac gagagatgag tttcaaaaac
      721 tattgcatgc agcaactatt gcctggggac caattggtta caagaaggta tttataaagg
      781 aaaataataa tataatagga tatttgataa taagaacaat aaaaaaagaa gattctactg
      841 taggagaagt tgctgaaata gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg
      901 ttgcaaataa atttggatta gaatatttaa attataaagt acatgtgaaa aatcttaaag
      961 accagttgaa atgtaatggg acaaaatctt tggattatca acaaggaact atgaaaataa
     1021 taaattttac taagttgtgt gatagtttaa gaagttattt tagtcaatat gtagattttg
     1081 aattactaaa atacatggaa tttaaacaag ttgaaaataa atatattatt aaatacaaag
     1141 aagaagaact tgtaatagaa aatttggata agcttaataa actgtttttt gaaaaaaatg
     1201 aggaacagta caatgacttt aaacatctaa aaaatatata tgaatttgct acaaaggcat
     1261 ttcctgtaga ttttccatgg actgcaaatt taaattatca atagttttag atgatattaa
     1321 ataaaattga ttgataccta gagttagtcc ttgttaaata agagaaatta ttggatattg
     1381 aaaaagcaat tttgtaagta attagaaagg atgagtatta tatggacata cttgatgata
     1441 tagcagaatg tatattaaat atgggtgtag ataatataga gaatttagta aaaattgcta
     1501 tagataaaaa tattgatgtg gaagatattt atgaatatgg tttaaacaaa ggtatgatag
     1561 gagctttaga taagtttgag aataaggaat actatctttc agaagttata gtatgcacag
     1621 atgcattaaa taaaggtatt aatcttttga agggaactgg aaaagtaaag aaaaaatcaa
     1681 agggagtaat tctgatgtct gtagtagaag gagatactca tgagatagga aaaaatattg
     1741 taaaagtaat ggtagaagcc actggatata aggttattga cttaggtgta aatagaaaaa
     1801 gtgaagatat aatagaagaa gctataaaaa acaatgtaga tataataggc ttatcttcaa
     1861 tgatgacaac tacaatggaa aatatgaaat cggttataga cgaacttaat atgattgaga
     1921 tagataaaag accaaaagta ataattggtg gaggacctgt aagtatggag tttgcagaag
     1981 aaataggagc agatggatac agttcaaatg ctccaaaagc agttaaatta ataaataaac
     2041 ttataggagg tgaagtttaa atgttttcag atatgacttt acttcagtat ataaatagtg
     2101 aagatagagg tttttttctg cctgatatgg gtacaaatgg attgttctta ataaataaaa
     2161 aggcatatga agtatatgat agtgtagatt tgcaattaga attagcaaag actatggaca
     2221 agtattttga atcagatttt atatactcat tctgtgatgg gattacattt tgtgaaactc
     2281 tgggtttaga gacattaaga ccagattatg attttccaag tgtattgaca catactataa
     2341 ctgacagaga taaattaaga aaattagatg ttccagaccc ttatacgagt ggacgtatgc
     2401 cactcaatat agagagctta tcagttatat caaaaagtat taaaaaacca ctatatgaat
     2461 cgatacaagg tccatttaca cttgcaggtc aattagctgg agctactcaa cttttaagat
     2521 gtataataac tgataaaaaa tttgtagaag aattattgga atttacaact gaattggtaa
     2581 gaagatatgc tgtagctgca aataaagcag gagcaaaata tatttcaata gcagagccaa
     2641 catcagtaac cctaagtaaa gatagatttg atgaatttat agtaaaaaat ttaaataaaa
     2701 tctacaatga acttgattgt tggaaaggta tgcatatatg tggagacacc agagaattat
     2761 tagataatat gctaagttgt aatatagatg cagtaagttt agaccaaatt ttagactatg
     2821 aagaaattgc tccaatgatt cctagagata tagttttgat tggtaattta gacccaataa
     2881 aacttcttgg acgttctaag ccagataaga ttagaagaga gacactaaaa ttgttaaaga
     2941 agatgagagg atatgataac tttttatgtg attttggctg taactgctta aataccactc
     3001 cagtagaaaa tttacaagca gctataaagg caggaagaat tagttataaa gaattagata
     3061 ggattgatat tgatgaatta taatagtaat aaaaataaat atcttttaaa ggatataaat
     3121 ttagatgata tttttataga taagaatatt gtgctaaagt ttttggggta tggaaataga
     3181 aaagctccag atacaattag taaaatcgtt gaaagagaaa taagaaatat aaatgatatt
     3241 ttagatatca agatatatat aagtgaagtt gatattaata aaacacctaa agaatgtaaa
     3301 aaagcatttg ctgtactata tacaataggg gataaaattg atattaagat gaatgactat
     3361 atggaaaatt gcaatatgat ggcagggcta gcattggata aaataggtat agtatgttta
     3421 gattgtatta atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag tctcaaaata
     3481 tcgcatgaaa tatatccagg agataaagat tttgaagtag agagacaaaa ggatatttat
     3541 aactatgtaa aaaataaata taatagtata gaaatagaaa tcaatgatta tcatcaacta
     3601 tcccccataa aaagtgttgc aatgcttatt ttaatggggg atgaagaaaa tttagaatca
     3661 agatgtagca aatgtcctag aaaatgcttt taactagttt tcaagactaa gatttgtttt
     3721 gtgtatgaat tgcaataatt tttttggtca ttttatgtat aaactaaaaa aattattgca
     3781 aagtaatatt ctatttaatt tgaacagact tacaaactat agtagtttct ttaattcaat
     3841 aagtgtattt atttcataag ttggtatatg attagtataa tttatactgt tattggaatt
     3901 taaccaacaa gtatctattc ctgaatttat tccacctaag acatcagagg ttaagctatc
     3961 cccaatcatt aaggcagaag atttatcatg acaattaaac tttttgagag cgtatttaaa
     4021 tatttcagga tttggttttg atattttcaa ctcttcagat acaaccattc catcaatata
     4081 ttcttttatt tttgaatttt caagtctttt cttttgaact gcagaaattc catttgttat
     4141 tataacaata gtatactttt ttttaagata ttgtaatata tcgattgcac ctggaattaa
     4201 aaatgtacat tcacctaaaa aatttaagta cattttgcta agagtttcag aatcaacatc
     4261 caaagatatt ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat tcaaagttat
     4321 aatatttttt tcaaggtcga gccaaagctt atcacttata tttctatatg actctatata
     4381 attctcaaaa ttaaattcta aatcaaactc agatagaagt tttttaaaag catgtgattc
     4441 acttttctta aaatcaaata aagtatcatc tgcatcaaaa aatataaatt tatatgtctt
     4501 catggttttt actcctttac aagatgactg caaaaattac tttactttca ctatttgagt
     4561 cattttgcca tttatgtttt gttcctaaag gaacttttac actatctcct ttggacaaag
     4621 taaattcatc atcatccata aaaagtttaa cttctccctc caaaacatat gcaatttcat
     4681 ctccattatg agtaatccta tcaacacaag aagaagtgtt tggagctaaa tccataattg
     4741 caaattctaa attaccacta gagtctggaa ttaaaatctc atatataact tctttgcttc
     4801 caggtaaaat aacttttttt ctattatcat gtcttactat caagtttttt ttatctactt
     4861 cagatacaaa aaatgtaaat aaaggaacat ttagagaaga ggctatagat tttaatgaat
     4921 ttacagatgg attagcaaga cctcgttcta tttggctcaa taatgatggt gtaacatcag
     4981 ctagttttgc taaatctcga atacttaagt tttgtttttt tcgaacttca gctatttttt
     5041 cccctaaatt taaatcattc atataaatat cctctctttt taatttaaaa aatattaact
     5101 taaaataatc aagtaaaatg attatgtagt atgaaattat tataacatat agaattaggt
     5161 tttgaaaaaa ctatgttcaa tataattaaa taaattaaaa tataattgac aaagtggata
     5221 cagaatactt aaaatatatt taaataaaat taagcaaaag atagttaatt tatgatagga
     5281 ggggatatta tgcaaataac gacttttgga gctatatttg gtttattaat agcaattatt
     5341 ttaattataa aaaaatttca agctgtatat agtttaatgt taggagcatt tattggagga
     5401 ttagttggag gagcaaatat aacacaaact gtagatttta tggcaaatgg agctatgaat
     5461 atatcaccat caattcttag agctttggca tcaggtgtat tagctggaag tttaataaaa
     5521 acaggggcag tagataaaat atcagaacag attgtaaaaa tatttggtga aaagagggct
     5581 ttattttcga ttgccatttc aactatggta cttgctggag taggcgtaaa tttggatgta
     5641 tctataataa ctgtagcacc tataggatta tatataggta gaaaattaaa ttattctaaa
     5701 ttgtcaatac tattagctat gttgggaggg ggtaaagcag gtaatataat atcaccaaat
     5761 ccaaatacta tagcagtagc agataatttt tctgtcaatc tctctagtgt tatgatggca
     5821 aatataattc cagcaattat tggagttgtt attacagtta ttttagcaag tatattaata
     5881 aataaaggaa ataaagtaca atcatatgaa attttagagc aaagagaaga tttaccaagt
     5941 ttatttaaat cattatgtgg tccaattata gctatatttc tattgttttt aggaaatgta
     6001 tctcctatag ttatagaccc aatgatagcc cttccaatag gaggaatcgt aactctaatt
     6061 gtcacaggaa acttgaacaa ttcaagagaa tatttagctt ttggattatc taagatgcaa
     6121 ggggtatgca tattattact tggaacaggg actatcgcag gaattataca gatgtctgaa
     6181 ttacaacaga gtacaatagg tgctttacaa tttttaaata tgccacaatt tttattggca
     6241 ccagtttcgg gtatacttat gtcattagca acagcatcat caacagctgg tgctactata
     6301 gcatcatcta cttttcatga tgctataata aatggtgggc tatcaccgat ttcaggtgca
     6361 tcaatagtaa atgctgggtc tagtgtattc gagcaattac cgcatggctc actatttcat
     6421 actagttcag gtagtataaa catggatata ggtgaaagat ttaagttaat tccatatgaa
     6481 gcattgatag gaatagtaat gactataatt tcaacatcta ttcaattagt tttataataa
     6541 atataaatat ttaatgaaaa aagtaggatt ttgttattaa atattgaatt tattatagta
     6601 aaaaagaaga ttggagtttt aaagtgaata aaaaaaagat tgttattata ggg
//
LOCUS       DAKBNN010000203         6581 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000203 DAKBNN010000000
VERSION     DAKBNN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.208"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..2205
                     /locus_tag="OUB02_003293"
     CDS             <1..2205
                     /locus_tag="OUB02_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCT8887021.1"
                     /translation="SLERLQGRLNNPLNAGNIQASQQSLNNIESILTRIEQKIGKADE
                     EQRKFNEDINKGASSTNNLLGSVKKLAGAYIGLKTVGGLGSLSDQMTSTNARLAMIND
                     GQLSDGGLNKMIFQSAERSRASYLDTAQIVSRIGMNAGSAFSSTREIVSFAEQLNKKF
                     IIAGASTQEMSSALLQLTQGLGSGVLRGEELNAVFESAPNIIKSIADYLDVDIGKIRG
                     MASEGMLTADIVKNSLLAASAETNKQFEQMPYTLGQIFTSVKNNAVMIFGAIQKKIED
                     TVSSGGFRTFIVNITDSLYILGSVGYKVFNGIIDLLSSPAFSGFFNLMIVGVSLITQG
                     LGWLLTTIGSVINFIAQGWSIIQPLLITSIVLWGLYKAAILAGVAITAIQVSWTALQA
                     FWTNLLNGSLLTNIMMNIAAKVSTDALSGSMLLLITTIVMVVATIALVIAAVFVGVAV
                     FNHFAGTSVSAIGIVAGAISVAAAFIGNLFVATGNLIIDIVALIYNTLAGFAEFFANF
                     LDDPIGSVIRAVSGMADAVLGILRSIASAIDTIFGSNLANAVSGWQDKLQGWTDKVAG
                     EAKIKVERMDPNKLHFERFNYGKAWDAGYKWGDKLETNIKDKFDISKIAEDAKKKLGL
                     DDLWDKKYGLGDSIGSAGLNSPLNDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIA
                     EQEVINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            2221..2916
                     /locus_tag="OUB02_003294"
     CDS             2221..2916
                     /locus_tag="OUB02_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCT8887022.1"
                     /translation="MAYDFYLDGVQLPITPGKLEVKVTNKNKTIDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPAQPKTTNETGRPNVKIE
                     PKRVDSVNAPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKKVP
                     KYTIYKGQVLRLV"
     gene            2909..4864
                     /locus_tag="OUB02_003295"
     CDS             2909..4864
                     /locus_tag="OUB02_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCT8887023.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYNNQTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNANNKDEKKNGPVQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDVTYQIQGNNQSSSTNNSSKTDKLINVAKSKLGCKYVYGATGPNTFDCSGFTQWCYK
                     QIGIKIPRTVATQSKAGSAVDLKDRSKWKAGDLLCRVGGGSSNHVMMYIGNGQMIHSP
                     QTGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            4878..5138
                     /locus_tag="OUB02_003296"
     CDS             4878..5138
                     /locus_tag="OUB02_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HCT8887024.1"
                     /translation="MSQELLQIIKKTAIDAVETSNPMQIAFGTIESLNPLVVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            5143..5562
                     /locus_tag="OUB02_003297"
     CDS             5143..5562
                     /locus_tag="OUB02_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HCT8887025.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            5563..>6581
                     /locus_tag="OUB02_003298"
     CDS             5563..>6581
                     /locus_tag="OUB02_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCT8887026.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSN"
BASE COUNT         2584 a          792 c         1333 g         1872 t
ORIGIN      
        1 agtttagaga gattgcaagg cagattgaat aatccactca atgcaggtaa tatacaagct
       61 tctcaacaaa gtttgaataa catagaaagt attctcacaa ggatagaaca gaaaattgga
      121 aaagcagatg aagaacaaag aaagtttaat gaggatatca ataaaggagc aagttctaca
      181 aacaatttac ttggaagtgt taagaaacta gcaggagctt atatcggatt aaagacagtt
      241 ggaggcttag gtagtttaag cgaccaaatg accagtacaa atgcgaggtt ggcgatgata
      301 aatgatggtc aactctcaga tggagggtta aataagatga ttttccaatc tgctgaaagg
      361 tcaagagcat cctatctaga cacagcacag atagtaagcc gtataggtat gaatgcaggg
      421 agtgcatttt ctagtactag agaaatagta agctttgctg aacaactcaa caaaaagttc
      481 ataattgcag gagctagtac acaagaaatg agttcagcat tattacagtt aacgcaagga
      541 ttgggaagtg gtgtacttag aggagaagag ttaaacgctg tatttgagtc agcacctaat
      601 attatcaaaa gcattgctga ctatctcgat gtagatatag gcaaaattag aggtatggca
      661 agtgaaggaa tgttaacagc agatattgta aaaaattcct tgcttgctgc atcagctgaa
      721 acaaataagc aatttgaaca gatgccttat acacttggtc aaattttcac tagtgtaaaa
      781 aataatgcag ttatgatttt tggagcgata caaaaaaaga ttgaggatac agtctcaagt
      841 ggaggattcc gaaccttcat agttaatata acagattcgc tatatatatt aggctcagtt
      901 ggttataaag tgttcaatgg aataatcgat ttattaagta gtccggcatt tagtggattt
      961 tttaatctga tgattgttgg agttagctta attacgcaag gactaggatg gctattaaca
     1021 actataggaa gtgtcattaa ttttattgca cagggatgga gcataataca gcctttatta
     1081 atcactagta ttgtattgtg ggggttatac aaagctgcta ttttagcagg tgttgcaatt
     1141 actgctattc aagttagttg gacagctctg caagcatttt ggactaattt attaaatggt
     1201 tcattattaa caaatataat gatgaatata gcagcaaaag tatctacaga tgctttaagt
     1261 ggttctatgt tacttctaat tactactata gtaatggttg tagctacaat agctctagta
     1321 atagcagcag ttttcgtagg agtagcagtt tttaatcatt tcgcagggac tagtgtatct
     1381 gctataggta tagttgcagg tgcaatatca gttgcagctg cttttatagg aaacttattc
     1441 gttgcaacag ggaacttaat tatagatatt gtagctttaa tatataatac tttagcaggt
     1501 tttgcagagt tctttgctaa ttttttagat gacccaattg gctctgttat aagagcagta
     1561 tctggaatgg ctgatgcggt attaggtata ttaagaagca tagcatctgc aatagatacc
     1621 atatttggct caaatctagc aaatgcagtg agtggttggc aagataaatt acaagggtgg
     1681 actgacaaag tagcaggaga agctaagata aaagttgaac gaatggaccc taataaacta
     1741 catttcgaac gatttaatta tggaaaagca tgggatgcag gatataagtg gggcgataag
     1801 ctagaaacta atataaaaga taaatttgat attagtaaaa tagcagaaga tgcaaagaaa
     1861 aaattaggct tagatgacct ttgggacaaa aaatatggtc ttggcgattc gataggctca
     1921 gcaggattaa actctccgct gaacgatgca gcaaaaggag caaaggacac agcaggaaac
     1981 actgcaaaaa tggctaaaac aatggataaa agccaagaag accttaaata tcttagagac
     2041 atagcagaac aggaggtaat aaaccgattt acaggagtca acataaaaat tgatatgaac
     2101 aatacaaaca acataaatag cgaggcagat gttgatggaa tagtaaatgt actaacagaa
     2161 aaactgaatg atgcaatggt tgtttcagcc gaaggaatag tttagagagg gggaatataa
     2221 atggcttatg atttttacct agatggagta caattaccaa tcacacctgg taagcttgaa
     2281 gttaaagtga caaataaaaa taagacaatt gatttaataa atgttggaga agtaaacata
     2341 ctaaaaaaag agggattatc tgaaataagt tttgaagcag agtttacaca taataaacta
     2401 ccattttatc gtggaacttt tagggatgtt caattctttt taagtaagct agaattacta
     2461 aaaactgatt gtaagccatt tcaatttatt gtctcgaggg aaatgggtgg aaaagtacta
     2521 tttaacacta atataaaagt atctcttgaa gagtatgcta tttcagaaga cgcagataat
     2581 ggttcagata caaaagttgc aataaagtta aagcaatata gagattattc aactaaaaag
     2641 ttagttcctg ctcaacctaa aacaactaat gagactggca gacctaatgt aaaaatagag
     2701 cctaaaaggg tcgattcagt caatgcacca aatgctaaaa catacacagt caaggcaggg
     2761 gatagtcttt ggtcaatttg ccaaaaacag ttaggtaatg gctcactgta taagaaggta
     2821 tatgagttaa ataaatcaat gatggataag gctaacaagg gcaaaaaagt acctaaatat
     2881 accatctata aagggcaggt gttgaggctt gtctgatgaa ttagttttgg caaatgatag
     2941 ggatgtaaga ttagtcatag cacattggga agatttctac gaacctgtag ttttggatgg
     3001 tatcacatgg gaaattgaaa ggcgaggaac accttcaaag ttagaattta caatagtcat
     3061 ggatgatata ttagagtttt gcgaaggtaa ctcagtaaga ttatattaca aaggtgtagg
     3121 catattttat ggatatatat ttcaaaagaa aagagataaa gaaaatcaca taaaaattgt
     3181 tgcttacgac cagttgagat attttaagaa taaagatact tatgtatata ataatcaaac
     3241 tgcatctgaa cttgtaaaga tgttagctaa agattttaat ttaaaataca atgtcataga
     3301 agatacaaaa tataaaatat cgagagttga agaaaataaa acactctttg atatggtctt
     3361 aactgcacta gatgatactc taagagagaa aaaggaaatg tatgttttat atgatgattt
     3421 tggaagaata acattaaaga atgttgcatc aatgaaatta gatactgtta tgaataatga
     3481 tgtcatagag gactttgact ataattcaag tatagatagt gatacttata caaaaatcaa
     3541 acttgtaaga gataatgaag aaacaggaaa aagggatgta tatattgctc aagactcagc
     3601 tcacatgaga agttggggaa tacttcaaat gtttgataca gtagacaaaa acatgagtga
     3661 agcagagata aaacaaaagt gtgatatact tctaaaacta tataataaga aaactaagtc
     3721 attaagttta aaaaatgcac ttggagatat tagagtgaga gcaggttgtt tagtacctgt
     3781 ttttttaaat ctaggagata ttgaattgca aaattatatg ttagttgaga aagtaaaaca
     3841 tacatttgaa aataattcac atttcatgga tttgaccttg gttgatggag acgaatttgc
     3901 ttcttattcc tcttcaagct acagtagtgg aaatgcaaat aataaagatg aaaagaaaaa
     3961 tggtcctgta caaagtacca caagtaaaga agatactgat atggctaata agattaataa
     4021 actacttaaa ggtaaattat caaatacagg aaatatattt gttaaatatt caaatgctta
     4081 taaagttaat ccggcactca tggctgctat atctatgcac gaatcagcta gagggacttc
     4141 aaatattgca aatactaaaa ataatttctt tggaatgaaa aaaaatggag attacatgag
     4201 tttttctagt gtagacgaag gaataaaaag aggtataagt aatttatcaa gaaactatat
     4261 ccatatagga cgaaaaactt tagaaagcat cagaaataaa tattcttcta gttcagacaa
     4321 agaatgggta aaatgtgtag gtgcatttta taagcaaata acaggaagta cttataattc
     4381 taatagtgca ggtacaggag ttggaagtaa cgaagaagca gaaaagaatt taaaagatgt
     4441 aacttatcaa attcaaggca acaaccaaag cagtagtaca aataatagct ctaaaacaga
     4501 taaattaatt aatgtagcaa aaagtaaatt aggttgtaaa tatgtgtatg gagctacagg
     4561 acctaatact tttgattgca gtggatttac tcaatggtgt tataaacaaa taggtataaa
     4621 aattcctagg actgttgcaa cgcaaagtaa ggcaggcagt gcagtagatt taaaagatag
     4681 aagcaagtgg aaagcaggag acttattatg tagagttggt ggaggtagta gtaatcacgt
     4741 aatgatgtat attggaaatg gtcaaatgat tcattcacca cagactggtg atgtagtaaa
     4801 aatacaatca gttgattcat atagaaaagg aaaagcatat acacatgtga gaagatttat
     4861 ataagtgagg tgataaagtg tcacaagaat tattgcaaat aattaagaag actgcaatag
     4921 atgcagtaga aacaagcaac ccaatgcaga ttgcatttgg aactatagaa agtcttaatc
     4981 cattagtagt taagatagaa caaaaactat ctattggtga aatttttcta atacaaacag
     5041 atacatttaa aagatataca gataaaaaga taggagataa attagtctta attaggatgc
     5101 aaggagggca acaatacttg attttagata ggatgtgatg aaatgttacc aacagataac
     5161 attgactatg atatagaaga tgtatcgata attaattttg atgttagaca agaaccaagt
     5221 aagacgttta aattgaatat agagaaaaat agagtagatg gtatttgtga tgatgtagaa
     5281 gcattaaaac aaaccatttt tttaatttta aatactgaaa ggtatgagca ccttatttat
     5341 tctagaaatt atggtgttga attaaatgat ttaattggag aacctatttc atatgtaata
     5401 cccgaacttg aaagaaggat aacagaagca ctaattcaag atgataggat tgaaaatata
     5461 gataattttg agtttcaaaa tataaagggt aaagtacaat gtagattttc agttcataca
     5521 aaatatggaa atataaaagc agagaaggtg gtgagtgtat aattgtttga gttaatgaca
     5581 tttgaaaata taattaaaag aatgttagat agtgtaccag atacttttga taaaagggaa
     5641 ggttctataa tatataatgc tcttgctcct gttgctatag aacttacaga aacatacatt
     5701 gctatggatg aattactaga ccaaacattc gtagatactg ctagttatta ttatttagag
     5761 aagagatgta aagagagagg tattacacca cttgaagcca ctaatacaat tgcaaaagga
     5821 gtttttaaca tagatattcc tattgattct aggtttaatc taggagaata taactatgta
     5881 gcaattgaga gaatatctga aggtatatat aagatgaaat gtgagactgc gggacctatt
     5941 tttgagttgg gacaactaat acctatcgaa tatatagaca aattagaaac agcagaactg
     6001 acagaaatac tgataaatgg agaagatgaa gagagtgagg atagtttaag acaaagatat
     6061 tatgatagcc taaattcaca gagctttggt ggaaatatgc aaaattataa agatgaagtt
     6121 aacaaaatac aagatgttgg aggagttaag gtttatcctg tgtgggacgg tggaggaact
     6181 gttaagttag taataattaa ctctaatttc aaagtaccat cagaggattt agttaattta
     6241 gtgcaagaag aaattgaccc aattggacat caaggacaag gcttaggatt agcaccaatt
     6301 ggacaccgag tcacagttga aggagttaca agtacaacta taaatatatc agcagagata
     6361 acatacaaaa atggctacac ttgggagaat ataaaatcaa ttgcagaaga agcaatagac
     6421 gactatttaa atgaacttaa catgagttgg gaagatgaag aaaacttaat agtccgtata
     6481 tctcaaattg aaactagatt acttagtatt gatggagtat tagacattgc aaatacaatg
     6541 ataaatgagg ttaaatctaa tctaacaata aatagtaaca g
//
LOCUS       DAKBNN010000204         6541 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000204 DAKBNN010000000
VERSION     DAKBNN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.209"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(104..655)
                     /locus_tag="OUB02_003299"
     CDS             complement(104..655)
                     /locus_tag="OUB02_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887027.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            846..1808
                     /locus_tag="OUB02_003300"
     CDS             846..1808
                     /locus_tag="OUB02_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCT8887028.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIILTLNDSGLKYLSTDLYEEL"
     gene            2103..3947
                     /locus_tag="OUB02_003301"
     CDS             2103..3947
                     /locus_tag="OUB02_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCT8887029.1"
                     /translation="MQKRILRNFIFTGKNILNKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICLNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIKEASHAKADYAEI
                     DVQEAKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDMYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4269..4853
                     /locus_tag="OUB02_003302"
     CDS             4269..4853
                     /locus_tag="OUB02_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCT8887030.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(5956..6132)
                     /locus_tag="OUB02_003303"
     CDS             complement(5956..6132)
                     /locus_tag="OUB02_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887031.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2543 a          645 c          984 g         2369 t
ORIGIN      
        1 tataatgaat ttataaagaa aaataaaata aaaaagataa atcctaatca aaggatttat
       61 ctttttgtat agaaaataat tattctatta ttaaatggca tggctaaact ggataataat
      121 ttattattac tgctttcaat gttttttctc cttgattatg gtatgtatga attgattcag
      181 ctgaaaaaca aatagagtca ttttctctaa gttgataaat agagtcattt acttctaatt
      241 ttaattgacc ttctataatc ataatatatt cctgtgattt ttcagaatga ccaacagatg
      301 tataactatg accttcttct aattccattt gaaataattc gaagtttcta gttggtgtat
      361 tgggataata acaataaaga cgataatctt tattttctga aacttgtaca tctatatcag
      421 tttttgatac aataaaagtt tcattttgag gttgttccag tattgcagta tatggaacat
      481 ttagtccact tgcaattttc caaattgtgt ttactgttgg gtttgagtct cccttttcaa
      541 tttgagaaag cattacttta cttacgccag ataattcagc aagttgacct agacttaagt
      601 tacgttcatt acgtaatcta tttaaatttt tagcaataac agtattaata ctcatagtaa
      661 ctccttaaaa tgtaaattat tgacaaatat aatttacgaa tgtataattt atttaacatt
      721 atttataatt tacaaatgta taatataatg aacgttaaat acactgtaca tccgtaatat
      781 aaattatact gcataaagag ttgtttttca acggatgata gttgagagat taagggggaa
      841 ttgtaatgcg tgtacttaat aatgttacag aggctatagg caatactcat atgttacatt
      901 tatccaaaat agcagatgaa tttggagttc aaggtaatat ttatgcaaag atggaatact
      961 taaatccggg atttagtaaa aaggaccgtg tagcattaca aatgattacc gaagcagaaa
     1021 atgcaggttt attagttcca ggtcaagcag ttgtggaatt aacaagtggt aatactggaa
     1081 caggactagc aatagcttgt gcatgtaaag gatataaatt tatagcgtgt atgtcaaaag
     1141 gaaattctat ggaaagagca agaatgatga aagctttagg tgctgaagta gttcttgtag
     1201 accaaatgcc taattcagtt catggacagg tatctggtga agatttggca cttgtagaaa
     1261 ttgaggctca aaaaatagca aaagaaagaa atgcatttag agcagaccaa tttaatttag
     1321 aagcttgtaa tcatgcacat gaaaagtata cagcagaaga aatgtgggaa caattggatg
     1381 gaaatattga tgtatttgta gattttcttg gaagtggtag tacttttgca ggatgttcaa
     1441 aaacacttaa aaaacataat tcttctatca aatgttatgt tgttgaacct gaaacagcag
     1501 caatttattc aggtaaagaa ataactgacc aagggcataa aatacaaggt ggaggatatt
     1561 ctatggattt gccattagta gacaagaaac ttattgatgg ttatattcaa gttacagacg
     1621 aggaggcaac tgatgtagca agaaaattag ctaaattaga aggtgtattt gcaggatttt
     1681 cttcaggggc aaatgtatgt gcagcaatta agttgttaaa gagcagtgaa aaaggaaaaa
     1741 atataatttt aactcttaat gatagtggtt taaaatattt aagtactgat ttatatgaag
     1801 aattgtaata atagcacaat agttatgcaa tgttttttaa attaagtata tagatttaaa
     1861 ttataaaaag tatctcaatg taatttttga aagttataaa ggagatactt ttttattttg
     1921 taaatatgga gtatcctact tataagtatt aagctatata gatattgatg attagctatg
     1981 caataaaaaa gagtatgtgt ttttgcattt atacatcata acttggaata atacatatta
     2041 atgttctaaa tgtatttaaa atatatgata aaattagaac aaatagatac aaggagataa
     2101 aaatgcaaaa gagaatttta agaaatttta tatttacggg taaaaatatt ttaaataaac
     2161 ttgtttttat aataaaatat aattttagta cattaatttt atttgaatta tttcataaag
     2221 gattagcctt attttttata tggccatcta ttagatatat atttgatatt ttaataaaaa
     2281 gtttacatat agtctaccta aatatggata attttataaa agttttgacc aatcccatgt
     2341 caatagttct gataatacta tcagttataa tactagcttt ttatgcattt tttgaattta
     2401 cctctgtaat tatttgttta aataaatcga taaaatatga aaaaataggg ttatttgaac
     2461 tctttaaaat atcatttgag aactctataa aattgttata tcctaaaaat atactattat
     2521 ttatatttgt gatattaata attccattag taaatacagt tttaatttca gggtttatag
     2581 gaaaaataca aataccagag tacatactag attatataaa gtctgatata attttaaata
     2641 taatctatat gatttttctt ttaataatat atatttcggt tattagatgg atttttagca
     2701 ttcatgagat aactttaaat acagaaaatt ttaaaagagc aagaaaagaa agtgttaatc
     2761 ttataaaggg aaagattata agaacaatta tttattcatt aatattatct ttagtagtag
     2821 ctattattgg atatgcgatt tatcatagtg gaattatatt tataggcata tggactaaat
     2881 attgtactga tatggaattt ttgaagaatg tatttataaa tagatgtatt gcatttgaag
     2941 aatattttag attgatttta tcaatattca tttttatatt ggacattgga tttatctcag
     3001 caacttatta tcaatataaa ggagtaagta tttctaaaaa taatattaaa aaagagaaaa
     3061 aatctatatc aaaaattgtt tttaatttag tattaatatt attgatttct tatatagagt
     3121 cagttgcatt ttcttcacat ataaatggta tgtttaatac gtcattttca ttttatacaa
     3181 cagcaattgc tcatagagca ggagctgaaa tggctccaga gaatactttg gcagcaataa
     3241 aagaagcttc acatgctaaa gctgattatg cagaaataga tgttcaagaa gctaaagatg
     3301 gagaacttat tgtacttcat gattcaaact ttaagagaac tacaggagta gataaaaatg
     3361 tttgggatgt aaattataat gaagtaaaga catatgatgc gggaagcttt tattattatg
     3421 gaaaaggtta ttatgatgaa aagtttgttg gggaaaagat accaacactc aaagaaatga
     3481 taaaatattc taaaggtaag gtgaaattat taatagaaat aaaacttaat ggacatgaaa
     3541 agggagacgt agtaaaaaaa gttgttcagt taattaaaga aaataatttt gaaaatcaat
     3601 gtatagttgc atctatggat aaaactatat tacaaaaagt aaaaaaactg gatgagaatt
     3661 tattgacatg ctattttatg gcaattgcat atggtgattt ttcaaagtta aattatgttg
     3721 atatgtatgg aattgaatca acttttgtta ataaaacagt agtagaaaag attcataaaa
     3781 tgaacaaaaa aatcttcgtt tggactgtta atagtgacag tttaattgag aaaatgcttg
     3841 acctaaatgt agatagtatt attacagata atccatacct tatagagagt gcaatttatt
     3901 ggaagaaaaa tggatttatt agtcaagtgt cagattattt attttaaata ttctataatt
     3961 gtattatcca gtaaaagaga tattattaaa atcaataata tctcttttta acataaataa
     4021 ggttgttaaa ttaaggttat tcttataaaa attgtgctta tcagctataa acaaggaata
     4081 aaagtgaaaa aattgctctg agattattac agttaaaaga gttttaagat gacagagaga
     4141 tagattgtca actgtatatt taataaatct tgttatttat taaattttat ataagtaaaa
     4201 tatataaatt aataaatctt atgctagaat atatatgaaa aacaatataa atttggagga
     4261 ttaaaaacat gacagagcga gaaaaaatgt tggcaggaga actttacgat tgtggtgata
     4321 ttgagttgtt aactcaatgg cataaagcaa aagacctagt aagagattat aaccaaacag
     4381 attcctcgga tttggaaaaa aaagaaaaaa tattaaatga gttacttggt ggtaagggta
     4441 aaaacttatg gattactgca ccgtattatg tagattatgg aaataatatt tattttggaa
     4501 ataattgtga agtcaatatg aattgtacgt ttttagatga taataaaatt atcattgggg
     4561 ataatgcact aatcgcacca aacgtacaaa tatatactgc atttcatcca actaatgctg
     4621 gtgatagatt tggtaatgcc aaagaagatg gttcatttga attttgtaaa actcaaacag
     4681 ctccagttac tattggagat aatgtttgga ttggcggggg tgctattatc atgccaggtg
     4741 ttactattgg agataatgta gttataggtg caggaagtat tgttacaaaa gatattccaa
     4801 gtaatatgat tgcatacgga aatccatgcc gtattattcg agaaaataaa taaagttaaa
     4861 ttttcttgca aaatctcaaa aaacaccttt cttactttat acatagggta ttttctaata
     4921 ctttcacatc catattcatt tgttaaattt ttgtggaatg tagtttttat cacttctatc
     4981 ttacatttcc accatttttt tataaaagtc attcctttat atatttattt ataatagtat
     5041 atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa cttttaggtt
     5101 agtattttac tatagtatga tgattaagta agaataaaat actataatac ataaagctta
     5161 ataaatataa tttttatttt acttagatat tttcttttga ttagtaatca aaagtggtta
     5221 taattttttt aattaataag agtaacattt attttatgaa agaggattat attaatatat
     5281 atttaaatag gaaaaattgt aaaaagcata aaaataatat aattaattaa tgagataaaa
     5341 gtggataaaa atattgggag tttaattttt gtgaaattaa aaatattaaa gataatctac
     5401 aaatatagaa tatattctaa gacgaatttt tataattact aaataaaata aattaatcaa
     5461 tatagttttt taatataaac aaaaaataaa aaacttatat aaataaaatg cttgtttttc
     5521 ttgtattatt tatataaata ttgaaatttg tctagttata cgtttttatt gaccagttat
     5581 gcagttgata ttgaatattc taaatattgg tgttatttta taggtggttt aaacatatta
     5641 gggggatttt atatatgatt tgtatgattt atagagaatg tataaaatag taaaggttta
     5701 gagccatgtt tagtggattg cagaatatgc atatagaatg attcttgtaa attaagttag
     5761 acataggaaa tatataattt gtattggatg attgatttta tggtgtatgc ttttaacttc
     5821 tataatggga aagtaaagat gtactcccaa tacatcttta ctttagcaga agttatagtt
     5881 acttataaag tacaaaaaat tatatattta ttagagtaga taaatctaaa aaggtttttc
     5941 tagaaaaatt tagttttaat tgtatctact tactaataat aagacttgat gtaagcaggt
     6001 ttcaactacc gtttctccat attctgatat aaaatcatct aactgtgtat ctaaaaaatt
     6061 acaataatca tatgaactac tgatgtaagc agttagtata tgtgtacatt tgtctatttc
     6121 tccaataaac atattatcca tccccctaaa aaatttagta aaaataaaaa gtcgcattct
     6181 attagtcaat taacataatt tataaaattt tttagtacta gtttctataa cttaactcct
     6241 taaggttaaa tattcccagt tttaccaatt tagttttatt ctacaagtat ataatactag
     6301 aagatgcttc attattcaac atgcttgttt gagaatagaa aatattctaa aattataatt
     6361 gtttttaaaa ataagattca agataaataa agttatatgc aactatttaa taatctttcc
     6421 ataataagga cagtaggttc tattcttaaa aaactccatc tttaaattta aagaataact
     6481 acaatttgaa caacaataaa atttccaatc aataaaaata tcaaaaataa taaaaataat
     6541 t
//
LOCUS       DAKBNN010000205         6484 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000205 DAKBNN010000000
VERSION     DAKBNN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.210"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..1010)
                     /locus_tag="OUB02_003304"
     CDS             complement(<1..1010)
                     /locus_tag="OUB02_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HCT8887032.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FD"
     gene            complement(1158..1949)
                     /locus_tag="OUB02_003305"
     CDS             complement(1158..1949)
                     /locus_tag="OUB02_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCT8887033.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1985..2641)
                     /locus_tag="OUB02_003306"
     CDS             complement(1985..2641)
                     /locus_tag="OUB02_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887034.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2634..3599)
                     /locus_tag="OUB02_003307"
     CDS             complement(2634..3599)
                     /locus_tag="OUB02_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCT8887035.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3705..3811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4101..5045)
                     /locus_tag="OUB02_003308"
     CDS             complement(4101..5045)
                     /locus_tag="OUB02_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCT8887036.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            5156..5623
                     /locus_tag="OUB02_003309"
     CDS             5156..5623
                     /locus_tag="OUB02_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887037.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(5793..6392)
                     /locus_tag="OUB02_003310"
     CDS             complement(5793..6392)
                     /locus_tag="OUB02_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887038.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2128 a         1066 c          667 g         2623 t
ORIGIN      
        1 ttatcaaatt tcttcaattt ttcagcaact ttttcatctt cctttataaa agttaatatt
       61 ttataataat tattcatatc ctttgaaaat tcatctttaa atttattatt tgtgtaactt
      121 atattaaaat catagtctgt tactatccat acatttggta ttgtatccag agcttgtcta
      181 agtaattctt ttctctggtc tatgtagttt tttaatgttc tttgtataat catatccttt
      241 tctattgcta aatttatctt tcctaattgc tctgactttt tacttaattt tttattgatt
      301 gaatcaagtt cataatatgg atacactact ctattattaa gactgtatac atatacataa
      361 taagtatggt tacataatag taaatatatt actcctatta atctatctga atctactata
      421 ttaatcttag aaattgctac aaaacatata atagttgtaa aaaaaacaaa atatgttaaa
      481 tcttttatta acttatagtg ctcaatgtta ctatcaagtt tattcaacaa atatagtaaa
      541 ttaaacatag atatacaaat attaattaat ataataatca tattccagtt aatatcacta
      601 aaaatatttc ttataaagta atctaatatc acaaaagcac tataaaaaat tagtattggg
      661 cttattctta gtttttttac tttagaaatg gttaagtata taaaagtaga catattaatt
      721 atgcttaagt taagcaatac aatttggaat ttttgaattt ttaaatttac tactcctaaa
      781 atacttacaa aaactaatac aagacaccct actttcaata tatttataaa ttcatccgtt
      841 ttaaagctac ttatataaac tatagaatat ccaaatatcc aaagtaaaat tattctatac
      901 atatttatag aaactgtata caaatttata tttaaaagtg caataaccaa aaataaccca
      961 ttagctagta tatagacctt attcttactt aaagttcctt tagattccat attccccacc
     1021 acttcttcat ttttatatag ttttacctat aaatatgtta ccacaattct tccaaaaaat
     1081 ttgtcacttt ctagttataa aaaaaaatga gtaaatattt ttaattttac tcattttgta
     1141 gattttgtat attcagttta aaaagctgga actatacttc ctttatattc atcttgtata
     1201 aattttttaa cttctttact attcatagcc tctgataaag ccttaatctt atcactatct
     1261 ttattatttt ctctacaagc tattatattt acatatggag aatcacttga ttctattact
     1321 attgcatcct tagtaggatt taagttagct gttaaagcat aattagaatt tattactgct
     1381 ccgtcaacat cttttaatac tgttggtaat tgctctgcat tcatttcttt tattactata
     1441 tttttaggat ttttagttat atctttttta gttataagct ctccatcttt tacttctatc
     1501 aatttatttt ttgctaataa tttaagtgct ctagctccat tagttgcatc atttggcaca
     1561 gctataacag ctccatcttt taattcatct aatgacttaa ctttttctga atagaatccc
     1621 attggctcta tatgaacttt agatgtataa gtaagtttat atcctttctc cttcacagtt
     1681 tcctctaaaa atggaatatg ttgaaagaag ttagcatcca aagacccttc atctaaagct
     1741 gtatttggaa gtacataatc atcaaatatt tttacttcta aatcatatcc tttttctttt
     1801 aacaaaggtt ttgcaacttc caatatcttt gcatgtggat ttgatgaagc tcctactagt
     1861 atttttttat cctctttgtt tgaacaacct actgctgata ttgctattgc tgacaccaat
     1921 gctactgata ataatttctt taatttcatt tttactcccc ctctttaaaa tgtttataat
     1981 taatttattt tagtttctta taagccagat ttcctactcc ctggattatc tgaactaata
     2041 ttataagtgc tattacagta tataccatta ctgctgtatc aaatctttga tatccataaa
     2101 taagtgctat attacctaat cctccagctc ctactgcacc tgccatagct gtataaccaa
     2161 gtatagatat tattgataat gtaattccag atactattga aggcatagct tcctttatca
     2221 taactttaaa tactatttgt ctttttgtag ctccaaaaga ttttgctgct tctatcaatc
     2281 ctttatcaac ttcatttaat gagctttcta taattctagc gataaacggt gctgccccaa
     2341 tagttattgg cactattgca gcagtttccc ctatacttgt tccaacgatt aatctagtta
     2401 ttggaatcat tgcaactata agtatcataa atgggaaact tctaaatata tttactataa
     2461 atcctaatgc actatttatt gtgctatttg gttttaatcc atctggtttt gtaactacta
     2521 atatgattgc taatataaat cctaatattg ttgccactat agttggtact ataaccatat
     2581 acaaagtttg taacaatgca tttggaaata gtgttgttaa aaagtcaatt aaactattca
     2641 tttatcattt cctcccatct tatgccctta ctagttagat attgttttac ttgctctcca
     2701 cttttatctg aaatgtttat aataagtgaa cctaaaatat catctttaaa ttgttctaat
     2761 ttcccaaata ttatagacac atctatattt agttctcttg ccatagtcgt aataatagct
     2821 tcattagaaa tatctttagg gaatagtatc tttatatttg ttccttttgg caatattata
     2881 ctttcttcac ctattaattt tctaagtcct ttagtatttc ttaagaatat ttcttctgta
     2941 tctcctacct ctaatacttc tccattttcc attattgcta ctcttccaca aatctgcttt
     3001 ataacttcca tttgatgagt tacaactatt attgttattc ccaatttttt atttatatct
     3061 tcaagcaatg aaagaattga ctttgtagta tttgggtcta atgctgaagt cgcttcatca
     3121 caaagaagta cttgtggatt tagtgctaaa gctctagcaa ttgcaaccct ttgtttttgt
     3181 ccgccactta gatttctggg tttatcattt ttcttttcag atattccaac tacttctaat
     3241 aactctaaaa ctcttttttt aatttctgcc tttgagtatc caaaacattc taatggcaat
     3301 gctacattat caaatacagt ttttctttca agcaatgaaa agtgttgaaa aatcattcct
     3361 aactcttttc ttaaatctct catttgcttt tcattcaatg actttacttc tttatcagaa
     3421 acaagtacac tgccttcttg gtattcttct aatccgttta tacatctcag tagtgttgat
     3481 ttccctgcac cactatgacc tattattcca aaaatttctc cagattctat ttcaatactt
     3541 acatctttaa gcacttgaat ctttccataa tacttattaa cattttttat gctaatcata
     3601 aaatttcctc ccatatattc tgctcagttt ttatgtttat cctctcttat gtattacaac
     3661 aaaataaaag cccgagtata aactcaggct tagaaagcta tttatactca tctatcaact
     3721 atattagttg caggatttag caccaatgta tataaaatat actggttgcc gggtttcaaa
     3781 gggccagtcc ctccaccact cttgataaga cgttatttaa tttaattcta ttatcatttg
     3841 ttgctatgtt atatatgata tattcattaa tagtatttgt caataaattt atttaaattt
     3901 tttatttttt attatcgtta attaactatt tttaattagt tttctagttc ctaacacttt
     3961 atattttaat gtctatataa ctatagaaca tcattataag aaataaatta aataacatca
     4021 atctatctaa atcatctgta aagtaaatta agaaaattaa cattttatcc aaactctatc
     4081 ctactattaa ataaaataat ttaaatacaa tgatgattat atagctctag tactttagac
     4141 tttatcaaat gtttaagttc tataggttct attattgtag catttttccc aaaagttaaa
     4201 aaatattcag ccacccagtt aagtgtagat tttggtatat acatatctat gtatccacct
     4261 ttagacaaga ttttcaaccc tcttgccaac aaaaattctg tttcacaacg ttttactccc
     4321 tcatctgtca aattaatttt aatatgataa tctgtaccta cttccatatt cttaagataa
     4381 tcatgtatag aagataaaac cttatatttc ttacttgata agttttctat actcaaaatc
     4441 ttcacaattc tgtctacacg aaattcctta atagattctg attttataca gtaagctgga
     4501 caataccaaa gtccattcat ggcatacaaa ccaataggaa caatggtacg tacactattt
     4561 ttttgctttg atgaatattc aattttaatt gtagattcat tcattactat attaaacaat
     4621 tctttcaata atggtgttga acagtgccta tctggtgtcc aaaaaacaaa tttacgcctt
     4681 atattctcaa tattatattg aatatcattt ggtatacaat ttaaaaattt ttttaaaaca
     4741 gaaatagttt cttgctcaaa tggcaaatca ttataatatt ctagtgactg atatgcaaag
     4801 aatatagatt ttacctcact ttctgaaaat gcaatcggtg gaagtatacg ctctttaagt
     4861 acatgatatc caccagccgc tccaactttt gaatataatg gaaatccaat actctccagc
     4921 acctgtaaat ctcttagtat agtacgtttt gatacagaaa attcttctgc taattctcct
     4981 gctgtaaaac ttctttttgc atttattgtt atcatcagtt caattaaacg ctcagctctt
     5041 gacatattta tactcctaat ttaaatatag tgacaataat tgtcactatt atatgataag
     5101 atattcttac atttaaatca ataataatag taaagaaaga agggactgat actttatgga
     5161 aaataatact attaaggctg atgatattat caattattgt ctatcaaact tagatgatgt
     5221 tttattaatg aatagttggg gggaacgagc aatttattac aatcctaatg gtattttaaa
     5281 acgaggagta tatgttctta ccattaaaga aaaagacagt aatcatgata aaggttcgtt
     5341 agttagtcgt ccaaatgtat accgtgtgaa tataggatta aaaaaagaaa cttttattga
     5401 aatgtttgga tatattccaa agcgtccagg tgtaggtcaa atagttgata tggattttga
     5461 ttttacaaaa ttggacacaa tcatgccaca tcctatctac tcatggatgg gatggatatg
     5521 cgtcctaagc cctactgaaa agacatttga gaactttaaa actttaatag gagaatctta
     5581 caattttgca aaacaaaaat ttaaaaagag aaaaaacaag taaaaacata tgaaaataat
     5641 ccctaaataa atcataataa aaatattaag gttacaagat aaaaattaat atcttaataa
     5701 catgcaaaaa agcgaagcca aaaaatgtat tatttataag gcttcgctat atctcaaata
     5761 atttatttca aatttcaaaa ctatttattc tattatttct tattatttcc tgttattcct
     5821 aatctatcat tcatatcttt tatcattttt ctaaaaccac tacctatatc atttccaaca
     5881 tcttcagttc catgaactaa ttttataaag tcattttgac cttttttcat atcttcatca
     5941 gataccttat ctaatttttc tattctctta tctatatctt ttactaatct ttccccttca
     6001 gcgattaggt catcattcat tttctttaac tctgtatctt ctgttttacc gtctttttta
     6061 agagatgtta caaatgattg tattttatct ctactatctt tatatgcagc ctttacatca
     6121 cttagatatt tttcatttcc tggatattct ttatctttaa actgattatt tatactttct
     6181 ggagtatcat aaatagaata tctatttaga cctgctatat aatcattata gcttgttgaa
     6241 taataattca tatatccttc attattattt acagattcat taggattttt tcctgtattt
     6301 tcacttggtt tttcattatt tttagtacat gcagttatac ctatagctaa tactagtgct
     6361 aatcctacaa ataaagttct ttttaatttc atttttatta ccctcctaga tgttttatac
     6421 tataaaaaat tttaataatt ttctttctta atttagtatt tctatatttt tatattttat
     6481 ttac
//
LOCUS       DAKBNN010000206         6394 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000206 DAKBNN010000000
VERSION     DAKBNN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.212"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..958
                     /locus_tag="OUB02_003311"
     CDS             <1..958
                     /locus_tag="OUB02_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8887039.1"
                     /translation="SKVNMFFDGGKFIGGKSIIFSIIGIIFLIILVSGIAYGYWVTKC
                     ISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHKSDEIQNKTDIMREEWIS
                     NITHDLKTPLSPIKGYAELLTDSSTNYSPEKVHKYASIMLKNIEYTNTLINDLKLTYQ
                     LKNGIIPLNKNNYDLVRFLKELSIDILNYPEYESNIVEFKSNIDNINFDFDCTLLKRA
                     FSNLIVNAFTYGDKNTKVCVHITLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTK
                     QKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI"
     gene            1177..3654
                     /locus_tag="OUB02_003312"
     CDS             1177..3654
                     /locus_tag="OUB02_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCT8887040.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRILIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            3669..4355
                     /locus_tag="OUB02_003313"
     CDS             3669..4355
                     /locus_tag="OUB02_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887041.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            4629..5462
                     /locus_tag="OUB02_003314"
     CDS             4629..5462
                     /locus_tag="OUB02_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887042.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5500..5961
                     /locus_tag="OUB02_003315"
     CDS             5500..5961
                     /locus_tag="OUB02_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HCT8887043.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            5992..6240
                     /locus_tag="OUB02_003316"
     CDS             5992..6240
                     /locus_tag="OUB02_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCT8887044.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2458 a          689 c         1006 g         2241 t
ORIGIN      
        1 atctaaagtg aatatgtttt ttgatggagg taaatttata ggtggtaagt caattatatt
       61 ttcaatcata gggattatat ttttaataat tttggtttct ggaattgctt atggttactg
      121 ggtaacaaag tgcatatcaa atattacatc tgctgttagc gatatttcaa aacgaagcta
      181 tcttccagtt aatattaaag gtgtttttgc tgatatatat aatagtttaa atattttaaa
      241 tgaagaaatt cataaaagtg atgagataca aaataaaact gatattatga gagaagagtg
      301 gatttcaaat ataactcatg atttaaaaac tccactttca ccaataaaag ggtatgcaga
      361 gcttcttaca gatagtagta ctaattattc acctgaaaaa gtacataaat atgcttcaat
      421 catgttaaag aacatagaat atacaaatac acttataaac gatttaaaat taacctatca
      481 attaaaaaat ggaataatcc ctttaaataa aaataattat gatttagtaa gatttttgaa
      541 agaattatcc attgatattt taaattatcc agaatatgaa tcaaatatag ttgaatttaa
      601 gagtaatata gataatatta actttgattt tgattgtaca ctattaaaac gagcttttag
      661 taatcttatt gtcaatgctt ttacatatgg agataaaaat actaaagttt gtgtacatat
      721 aacattaaat gaaagaattg aaatcattat atctgataat ggaaaaggga tgtcaagaga
      781 agaagcatca aatttgttta atcgttatta tagaggaagc aatacaaaac aaaaaataga
      841 aggaactggt ttaggactag caataacgaa gcaaattatt gaagttcatg gagggaatat
      901 atctgttgaa agtatacctg gattaggtag ctcattttat atcacctttc ctatttaatt
      961 aaggttaatt aaggttaaat aaaaaatata ttaaggttga tagattatta tatttatata
     1021 gtagtttatc aatctttttt atttaagggg tgatttgatt aaaaatatta taaatagaat
     1081 gataaaagta tggaggagtc tattagagat ttaatttaga ttaaagtata aaaatattag
     1141 attgaaatat cttagataag gaaagaaggt gtaaatttga aaattatatt aaagtatatt
     1201 ttcacaaata ttaaagaacg aaaaattaga acagcagtta tgttattgtc catagtttta
     1261 tcaacagttt tactttttgt ttcattctca attggattat cttatgaaag tgcacaaaga
     1321 aaaatggcaa aaggtatgta tggtacagcg actatatctg tccaaagtaa aaatccagat
     1381 atattaacca atttagaaga catacctgat ttaaatgcta taaaatcaaa ggttggtgta
     1441 ttggaaagct ctgctattta taataaaggg ggatactatg aagaatttag tcttatatca
     1501 gcagacttaa gtcaactaaa taaaatcaac aagccacgat tagaaaatgg cgatagtatt
     1561 actgattttt ctggtgataa aattattctt ccaaatcgct ttacatcaaa atataaaata
     1621 aaaaaaggtg attctattac tttgcagatt tatggaaagt cttatacttt tcaagttagt
     1681 gatattgcat cttatgatac agtatttttg aggaacacaa gaggtgtaaa tgcactgcta
     1741 cccaaagaga ctctatcaaa aattataaat aaaggcagtg gatatacaag aattcttata
     1801 gagtcagaag aagaaattac agaaaatcta gtaaataaat tatccaaaga actttctact
     1861 gaaaaatata tggtatcaaa tactataaat gaaactaaga taatttcaga tgctagacaa
     1921 aagacaatgc ctttcttttt aataagtttt tttgcattaa cccttagtat atttataata
     1981 tacagcagtt ataaggttat tacattagaa cgtcttcctt ttattggaac atttagaagt
     2041 attggtgcca atgaaaaaac tgtaactcgt atattgatgc ttgaaagtat attatacgga
     2101 agtataggag gattaattgc tattcctatt ggtgtagttg ttttaaattt aatgcttcat
     2161 gggttaggaa attctctcga gcaaggaata tcaattccag tagtaatatc acccattgga
     2221 gtgattatat ctgttatagt agcaataatc gtttcttcat ttagtgcata tatacctgtt
     2281 aaaaaagcta gccatttgcc gataaaaaat attgtactag gaacagtaga agaaaagaat
     2341 gtttcaaatc gttctatact ttttatagga agtataatgt ttattttatc tattttactt
     2401 ccaagaatat cccctgaaaa tacattgtat ctagctggtg gattttcatt actgggatta
     2461 attgtagcta ctattgtgtt gattcctctt attactgata tcatgtctat agtttttgag
     2521 tttgtttata aaaatatttt agggaatgag ggtaaattag ctgctagaaa tatgaaaaat
     2581 aacaaaaata taatacagaa tatcacttta ctatttataa gtatttctgc agttattgca
     2641 ataagtgttg ttggtaactt tgtcaaaact tatataacag atgtgtttcg tgacgcagaa
     2701 ttacaaggtt ttgcagatgg aaagatgaat gaagagttta ttgaagatgt tagacatatg
     2761 gatggaatta aaaaaatatt acctctttat gttatgaata atgagatttc tggtaatggt
     2821 gtaacattat caagactaga gggtacagat aatataaaac tatataactc tatgtttgga
     2881 atcaattata caaattttga gataaaaaaa caagtaatag aagcttttaa tgataaacgt
     2941 tctgttattt tgaatgaaga tactctaaaa aaagtaggct tatctatagg agatacaata
     3001 actctatcta atgataagta tgatttttct tataaaatag taggtagttt taaatcccgt
     3061 gcaaatgatg tagaagctgt tataccatca cattatgcag ttaatgattt tgataaaact
     3121 aattatggtt ttttagtata tacagcagtc aatccagatg ctgttatgat tcagataaga
     3181 tatttatttg gagatacata taattggagt cgtacagttg aagaatttaa taatgattct
     3241 ttaaatacta taagtagttt tttgtctcct atgaataaga tgacctattt catttttctt
     3301 cttgctacag taggtattat taataatcta ctgattaatt atattcaaaa aagacgttct
     3361 atagctatgt ataaatctat aggactaagt gataaacaaa atataaaagt aacattgatt
     3421 gaagggttta cttcaggttt acttggagct gttattggta ttgtgatatc tatacttgag
     3481 attcaaacta tctttatagt tgcaggacca aaaatttcaa tgaaaccaga tttagatttt
     3541 aaaacattta ttattgttgg gttattggga attatagtaa ctttaatagg ttcaatagtg
     3601 cctattataa aagggaagaa aatgaaactg atagaagaaa ttaagtttga ataattatgg
     3661 aggttaatat gaaaattaca cataatagtt tagctgtaga agcaaaaaat attatcaaag
     3721 aatataaaat tggaaataca acaactcgag tgttaaaaga ggtatcttta caagtaatga
     3781 aaggtgaatt tgtttctatc atgggtcaat ctggttctgg taaaagtact cttttatata
     3841 ttttaggtgg tttagatact ccaacgagcg gaaaagtgta tatgaatgga gctgatatat
     3901 cacattttaa tgatgagaaa atgagcataa tacgtcgtag aaatatagga ttcgtgtttc
     3961 aattttataa cttaatacca aatttaaatg tggaggaaaa tataatgctt cctttgttat
     4021 tagatggtaa aaatttaaaa gattataaaa atcaattaga tgaaatctta gatatagttg
     4081 gtttaactga tagacgtaaa catacaccac gagagttatc tggtggtcag caacaacgtg
     4141 ttgctatagc tcgagcttta attggaaaac cagagatttt atttgctgat gagcctactg
     4201 gaaatttaga tagtaaaaca gggatagaaa taatagattt actgaataaa ataaacagag
     4261 acaatggaca aactataatt atggtaactc actcaccaga agcagccaaa agtagtagtc
     4321 gtactattac agtttcagat ggtttaattg tataaggact aattattgta tttgagataa
     4381 gcatttttaa ctatataaat tagacatatg taaagtggat ataagttaaa tactataaat
     4441 gtgctataat attaaaataa cagataaaag tactatattt tttattgatt attttaattt
     4501 tatttgttta ttaaatatct aatgagtatt catactatct ctattattaa aagcagttaa
     4561 tgatatatag agataatatg gattcttctt actttaataa atattcacga taggacagga
     4621 tataatatat gttaaaaaaa gaaagtattc attttataaa taaaaatagg tcacttatag
     4681 tttgtgaaga caattttgtt tatctcttgc cttgtataca attaagaaat tggatatcga
     4741 attttactat atcgtttcca aatcaaacaa taatttctga taattacacg attattcctc
     4801 atggaagtgt aactttggtg tttttttatg atgccacagg gttacatagt ctattatttg
     4861 gtccaactac taaaccaaaa acagtaggaa atattgctaa tagatgtgat gttatactaa
     4921 ttatagaatt tcaacctgct ggttttttcc cgcttatagg tatacagcaa agtgaactta
     4981 ttgataaagt tgttccattt tctataatta atacttcatt agatttagaa attaaaaaaa
     5041 tatttaatga atctttatct atagataaat tgattttaaa gttggaagaa ttattgattt
     5101 ctaatattaa aattgaatac tcatatgagt ttattttggc aattcagttg attattcaaa
     5161 actctggaaa tatatcttca caagaaattt ctaaaaaagt attttatagt tctaggcatt
     5221 taaacagact ctttaatcag tacctagggt tgagtatgaa gtcattttct cgtttggtac
     5281 gtattaataa atctataaaa cttctaaaca ataataaaac ttcattgatg tctatatgta
     5341 atgaattagg attctatgat ataccacatt ttattaaaga ttttaaaatt gtctgtggaa
     5401 taactcctca aaaataccga gctaatatgt ctgattttta cagtgaaatt gcaaaatttt
     5461 aagttataat acttataaca aagaaaggat agtgataaga tgaaatttgg aggagtattg
     5521 ctaattgtaa aagacataaa taaatcaaaa aagttttatg aagatgttat ggagcaaaaa
     5581 atcacattag atttaggaga acatgtatca tttgaaaatg gatttggatt acaaagtaat
     5641 tataaagcta taattggata tgatttacca atatatcaac aatcaaataa ctttcagttg
     5701 tattttgagg ttaatgacat caaacaatgg gaaagtaaga taaatagaat aggaaatatt
     5761 gaatttttgc attatataaa agaataccca tggggtcaaa gaacttttag gttttatgat
     5821 tttgataaaa atattataga aatttcagaa agtatggaaa gcgtcataaa gagactttta
     5881 aaacaaggtt tagctttaga agaaatttca aaacgcacta tgtatccagt tgaatttatt
     5941 acacaatttc aacaattata aaatattaaa atatataatt ttttctttat attgcaaaaa
     6001 acaaaggctt ggggcataat agaaccagaa aaacctgaat ctcaacaaca aatggctcat
     6061 atggatttcg ctgttaatga tttagaagaa gcagttcaat atgcaattca ttgtggagct
     6121 acaatcgcag aggagcaatt tactgatgac tggagagtta tgattgaccc agctggacac
     6181 cctttttgct tatgtcagat gaaatcaatt atggaaagta gccatttttc attgttatag
     6241 accaaaatat tgtttattaa ttacaaaaaa gcacaacagt atataccgtt gtgcttttac
     6301 ttattataca tgaatacact aaaaaatata aatagtagag ctttatcaat aatgataatt
     6361 tttaaaaaat agtatttttt tattgaataa tttc
//
LOCUS       DAKBNN010000207         6393 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000207 DAKBNN010000000
VERSION     DAKBNN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.213"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            53..769
                     /locus_tag="OUB02_003317"
     CDS             53..769
                     /locus_tag="OUB02_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCT8887045.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            848..2158
                     /locus_tag="OUB02_003318"
     CDS             848..2158
                     /locus_tag="OUB02_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCT8887046.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTRKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            2258..2794
                     /gene="lepB"
                     /locus_tag="OUB02_003319"
     CDS             2258..2794
                     /gene="lepB"
                     /locus_tag="OUB02_003319"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCT8887047.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            2960..3496
                     /gene="lepB"
                     /locus_tag="OUB02_003320"
     CDS             2960..3496
                     /gene="lepB"
                     /locus_tag="OUB02_003320"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCT8887048.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            3506..4261
                     /locus_tag="OUB02_003321"
     CDS             3506..4261
                     /locus_tag="OUB02_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCT8887049.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KDRISKLGLSIGIHLPMELNTCENIEYIRDSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            4335..6002
                     /locus_tag="OUB02_003322"
     CDS             4335..6002
                     /locus_tag="OUB02_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HCT8887050.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         2467 a          588 c         1251 g         2087 t
ORIGIN      
        1 gtagatgata taaatcataa ataaatcaaa aattttgaaa ggaaaataat ctatgaagct
       61 agatttattt ttattagcaa tagttcctat attgataggg atgttttgga taaggtctaa
      121 agatagatat tgcagagaac ccctaatcca tctaattaaa ttttttttaa taggtgcttt
      181 tctaagtgtt ataattatac ttttagaaaa tttactgatg aagtttaatg tatttgaagg
      241 gtattcagaa ctaatatatg tatcatttgt agttgcagga cttgtagaag aaggtgtaaa
      301 agctctgata ctgattcctg ccctaattaa agaaaaacac tttactgaaa agttagatgg
      361 tattatttac tctgtatttt tagctttagg atttgcaaca attgaaaata tggtttatat
      421 tttttctgaa agtagaaatc tagcactgca agttggtatt aatagggcag ttatctctat
      481 accagctcat gtaatgtttg caattactat gggctactat atttctaagt ataaatttga
      541 gggaaataaa aataaaagac gtgagtattt gtttatggca gtgttaattc caattttatt
      601 acatggagtt tttgatttca tattgatgat agaatataga tgggctatta tattacttat
      661 agtttatgta ataattttgt ggaagataaa tcttgataaa cttgagaaat atatgaatca
      721 ctctaaaaaa gtgtttttcg gtaatcttag gaaaaagaag aagaaataaa atttaaagga
      781 aagacataaa taattaaagt ataataaatg acacacaagt aaagcagatt gaagggggat
      841 tatagatttg acagaattac caaaggaatt tttagaagat attgaagaaa tactaaaaga
      901 tgaatatgat gattttataa aaagttatga ggaaagtaag actactgggc ttagaataaa
      961 tactttaaaa attgacaaga aaaaattatt tgatttggag ttgtttgatt tgaatcaaat
     1021 accatgggca gaagaaggtt tttattatga tgataaaatt gacagacctg gtagaaatcc
     1081 actgcatgag gctggagcat actatttgca agagccttct gcaatgagtg taataccaaa
     1141 ggcagatatt aaagagggtg aaaaagttct cgatatgtgt gctgctcctg gtggaaaatc
     1201 tacatatata ctatctaagc taaacgatac aggtttattg gtttctaatg aaattaatgc
     1261 tagtagaatt aaggctctag gggaaaattt agagcgattt ggagcgagaa attgtattat
     1321 aacaaacacc gattctaata gcttaagaaa gacgtttaat gggtattttg ataaggttat
     1381 tatagatgca ccttgttctg ggcaaggtat gtttaggaag gatgaggttg ccatatcaga
     1441 ttggagttat gcaaaggtag tagaatgtca atccatccaa aaagagatta taagagatgg
     1501 atataaaatg ttaaagaagg atggcatttt aatttactca acttgtactt tttcaaggga
     1561 ggaaaatgag catgttataa atgagtttat cagtgaatat ccacatgcag aacttattga
     1621 aatggaaaga ttatggcctc ataagataaa gggagaaggt cattttgttg ccaaaataaa
     1681 aaaattagaa gaagaagaat gtaatgtcaa agaattaaaa ataaaaaatt tagataaaga
     1741 aataaaggat tatagggagt ttgaaaagaa atttttaaag gttgattttt atagtaataa
     1801 aaaaaataaa ttttatttaa gaggagaaaa tttgtaccta cttcctgaaa tatatccaga
     1861 tacgagaaaa ctaaaagtat tgagatatgg tttgcattta ggggttttaa agaagaatag
     1921 atttgagcct tctcatgcac tatctcatta cttaaaagtt gaagatgtaa aaaatgttga
     1981 aaacttcagt gtacagagtg aatcaattct taaatattta aaaggggatg ttgtaaattc
     2041 taatgatagt agaggatggg ttttggtttc ggttgaagga atacctttag gttggggaaa
     2101 agaatcttct ggtgtaatta agaatcatta tcctaaaggt cttagaaaag tattttaaac
     2161 tattagaaaa aagaagtata atatttatat tgtaggttta tggtagatta ttttgctgta
     2221 gattgactta atatttaaca cagaggagac gattttagtg ggtgaagcag ttaaaaaaga
     2281 agttgtagaa tggataaaag tgattgtcat agctcttgtt ttggcatttg caataactcg
     2341 ttttatagtg ccaacaatag tcaaaggaga atcaatgtat cctacattag ttgaacgtga
     2401 ttatttgata gttaacagaa ttgcgtacaa ggtaggagag ccaaaataca aagatataat
     2461 agtattcaaa accgacttaa cagaggaaaa tggaaagaaa aaagatttag taaaaagagt
     2521 tatcggggtt cctggtgacc atgtaaaaat acaagactcc aaggtatatg taaatgataa
     2581 gttgttagat gagacttcct atatacataa taatcgtact gatggagata ttgatatcgt
     2641 agttccagaa ggaaaattat ttgcaatggg agataataga gaaaaaagtt tagatagtag
     2701 atacgatgag gttggattgg tcgacgagca taccatttta ggaaaggttc tagtcagatt
     2761 gtatccattt tctaagatag gaactattga ctaaacatat aaaatttgga gcaatcgctg
     2821 attagctgaa atcaccagac catacacaat agttgtctat ataaaaaaga caacacctta
     2881 tttagtagac ttatctaaaa gatagtgttt aagagacatt atcaataaag ataatataac
     2941 tataaaggag aatttgtgaa tgaatgaaac tattaaagaa gagattgtag agtggataaa
     3001 aataattatt actgcacttt tttttgcatt tattataact cgttttataa aaccaacatt
     3061 agtaaatgga gaatcaatgt acccaacact taaatcacat gattatttgg tagcaaacag
     3121 gatgacatat aagttatcag aaccaaaatg tggagatata atgatattta agactgattt
     3181 attacaagag aatggaagga aaaaagagct tgtaaaaagg gttataggtg ttcctggtga
     3241 ccatctaaaa attaaggatt ctaaggttta tataaatggt aagttattaa atgaagtttc
     3301 atatatacat gataattata ctgaaggtga tattgatatg gtgattccta agggaaaagt
     3361 atttgcgatg ggagacaata gagaagttag tttagacagt agatataaag aagtgggatt
     3421 agtagatgaa gaaaatatta aaggaaaagt tattttaaga gtatttcctt ttacagatat
     3481 aggtattttt gagtaggtga tttgtatggc agttggtata gcgtccttat tgtttaatat
     3541 tgaggaggct ttaaacattt gtgagagtat aaagcaaata actcatttag agattggaat
     3601 tgacaatata tctgaatgta gtgagttatg taaatataaa gacagaattt ctaagttagg
     3661 attatctata gggatacatt tgcctatgga attaaatact tgcgaaaaca tagagtatat
     3721 acgagattca tggataaatt ttattgagaa gatagaattt gaactaaaag gatttgattt
     3781 aaggtatttt aaccttcatt tgggatatgt tatgacaaat agagttagaa agaatagaga
     3841 caaatattta ggaaatagtg tggatttttt agataaatta aatacaaatt catatgtatg
     3901 tatagagaat acttattcaa aaggtgggga tttttcaaat attggaaata tatcttatga
     3961 ttttgaatat atatttaaga ggattaaaaa ttctaagatt tgtttttgtt acgatactgg
     4021 acactgttta attgatgagg atgcttatgt aaagaactta aaggataaaa taagactgat
     4081 acacttaagt gataatgatg gtatcaatga tacacatgtt ggtataggaa gaggaatttt
     4141 atctgaagag gggattaaag aggtcttaac attagatgca gaatatttag tattagaaat
     4201 aaattacgaa gacattgaag atactataag taaattgaat aatattgtag gagagggtta
     4261 aatacctctc tttatcttgt tgatatgcct cattaattaa aatttatatt catcaaaagg
     4321 aaggagatgt tgaagtgaaa aaaataaact taaaaataga aggaaaagat aaggaatatt
     4381 cattagaaga aaattcccct ggaatcagat tgggagatat agcaaaagaa ttttgtgatg
     4441 agcataaggg ttatataaca cttgcagttg tagacaataa actaaaagag ctaaattgta
     4501 gagttaagaa ggattgtgag ataaattttt tagatactac taatgaagat ggagagagag
     4561 tttactttag agtcatgtca tttatatttg taatggcatg tagagaaatc ttttgggata
     4621 gcagagttac aatagaacat tcactatcag atgggctata ttgtgaagtt catatagata
     4681 gaaagcttaa agaagcagat gttgaaaata taaaaaacaa gatgaaagag atagtaaaca
     4741 acgattatgt tatagaaaaa attgaagtta caagaaatga agctatttct atctttgaaa
     4801 ataatgaaat gtatgaaaag gcagagcttt tgaaatacaa agagtatgat agtgctaaag
     4861 tatataagtg tagaaactat atagaccatt tttatggtta tatgttgcca tcaactggtt
     4921 atataaaatc atttgatatt aagctttata atggaggcgt tataatttta ggacctagtg
     4981 aagaagataa gactcttcct atgaaatttg tacctcaacc taagctatca agtgtatatt
     5041 atgaagcaga agaatggtca agacttatgg gaattgacaa ggtagtatct cttaataaaa
     5101 taatagaaaa caatgaatat ggagatataa taaggacatt tgaagcattg catgaaaaga
     5161 agttatccca aatagcggat atgataaagg atggcaataa gagagtagtt ttaatagcag
     5221 ctccatcttc atctggaaaa acaagttttg ctcacagatt atcaatacat cttagagtaa
     5281 ataatttaaa tcctatttca atttctttag atgattattt tatagataga aaacatactc
     5341 ctttagatga atatggaaat tatgattttg aatcaattta tgcaatagat ttagagaagt
     5401 ttaacttaga cttaaagaaa cttttagctg gtgaagaaat tgatgatata agattcaatt
     5461 ttaaagaagg taagagagaa tacactggta aaaagataaa gatagattca aatcagccaa
     5521 ttatattaga aggtatacat gggctaaatc ctattttaac atcatctata ccagatgaag
     5581 ataaatttaa gatatacata agtgccctta ctcaaataaa tttggatgac cataatcgaa
     5641 tccctactgc tgatttgaga atgataagaa gaattgttag agactataat tttagaggat
     5701 atagtgctga taatactatt ttacaatggg catcagttag aagaggagaa aagaagaata
     5761 tattcccgta ccaagaggag gcagatgcta tatttaactc tgcttgtgta tatgaattgg
     5821 cagttttaaa acgatttgcg aaacctttgt tggaagagat aaaggaagat aatcctgcac
     5881 atattgaagc tactagatta ctaaagtttt tgcagtattt tgttgaattg gacgatactt
     5941 ctgacatacc aggtatttca attcttaggg aatttattgg aggaagtaaa atagttgatt
     6001 aatatgtgta ttatagattg ttttatgaag attaaatggc atagcaagta gtgtgattgg
     6061 tttatgttaa gtatattaag tgtaaagtgg agaattaaat tgatacagat tttaaagttt
     6121 gagttgatat ataaaaaggc ttgttttata cttttgtatg aagcaagctt tttattttaa
     6181 attttacttt tttgtttaat attttgttag tttatgtgat tattttttaa aagagtaaaa
     6241 gagtaaaaga gtaaaagagt aaaagagtaa aaggtgttgg gttagaaatt tttggtgtta
     6301 taaaaattta aattactgga ttattagatt gctggattgc taaattacta ggttgttaaa
     6361 ttattgaatt attaggttgt tgaattacta ggt
//
LOCUS       DAKBNN010000208         6345 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000208 DAKBNN010000000
VERSION     DAKBNN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.214"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            185..1129
                     /locus_tag="OUB02_003323"
     CDS             185..1129
                     /locus_tag="OUB02_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCT8887051.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1151..1777
                     /locus_tag="OUB02_003324"
     CDS             1151..1777
                     /locus_tag="OUB02_003324"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCT8887052.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1811..2860
                     /gene="hisC"
                     /locus_tag="OUB02_003325"
     CDS             1811..2860
                     /gene="hisC"
                     /locus_tag="OUB02_003325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCT8887053.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2863..3441
                     /gene="hisB"
                     /locus_tag="OUB02_003326"
     CDS             2863..3441
                     /gene="hisB"
                     /locus_tag="OUB02_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCT8887054.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3443..4048
                     /gene="hisH"
                     /locus_tag="OUB02_003327"
     CDS             3443..4048
                     /gene="hisH"
                     /locus_tag="OUB02_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCT8887055.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4045..4767
                     /gene="hisA"
                     /locus_tag="OUB02_003328"
     CDS             4045..4767
                     /gene="hisA"
                     /locus_tag="OUB02_003328"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCT8887056.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4749..5507
                     /gene="hisF"
                     /locus_tag="OUB02_003329"
     CDS             4749..5507
                     /gene="hisF"
                     /locus_tag="OUB02_003329"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCT8887057.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5535..6203
                     /locus_tag="OUB02_003330"
     CDS             5535..6203
                     /locus_tag="OUB02_003330"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCT8887058.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2588 a          531 c         1250 g         1976 t
ORIGIN      
        1 agggattgta atatgaaatt aattcataga cacaagccct gtttttagtg taattggtct
       61 tgtatctatg atgggaagta attttaagag aaaattatag ccatataggt ataaagccta
      121 tatggctttt ttattgtttt aaatttgcaa atataagagt aaatttaatg aagagggtga
      181 gaatgtgaaa tttttgagga ttgaagatga ggttatttat tctaaaaaaa gatataaatt
      241 aaaaagggaa atagataaac tttttttgga tgaagaatat tttcaaatag aaccacaatt
      301 atttgaagaa tatgatgaat ttacaactat aaataataaa attccagaag aatctatggt
      361 aaaggttgta aatggaaaag taatggtact tagagctgat ataactacaa gtataataaa
      421 aagccttgta ccaagatggg aagatggttt aaagcttaag cttttttata attcatcaat
      481 atataaaaat aaaaatacag ttggaataaa agagataaga caaataggtt gtgagtatct
      541 aggagaagcc tctgtagaag cagatagaga agtagtgaaa ttagctctta agatacttga
      601 aaaatacaat aataatttta ttctagaggt tgggagtagc aagtatattc atggtttgtt
      661 agaagaatta aatttaaata agaattgtga aaatcaaatt aaaaatttac tatatacgaa
      721 gaacactcat gaactaaaag tatatattga agaattaaaa attaagcgtg aagtaaaaga
      781 gttattatca aatattttaa gtttacaagg gaatttatat aatgttattg aaaaatcaaa
      841 caagttctat tgtaataata agatgaagca agctcttgaa gaattaaagc aagtaaataa
      901 tttaattgag gaatgtaatt ttttagataa ggctagattt gatttatcaa tgattactat
      961 gcttgactat tatgaaggaa ttatgtttag aggatactac ccaaactcat ataaagaaat
     1021 tcttagtggc ggaagatatg attctttaac taaagagtat ggaaaagaaa ttccagccat
     1081 tggatttaca ctaagtgttg atgagttaat gaagtatgta tataagtaaa gtttataagg
     1141 agggattgtt ttggattatt tgactatagc actagcaaaa ggacgaattg aaggggaatc
     1201 atttaaaaaa tttaaaaaaa tgggtctagg agatagtata gacacagata cgagaaaact
     1261 aatttttaaa gatgaagaaa ataaaattat atatatacat gtaaagccat cagatgtagt
     1321 tacatatgtt gaaaaaggag tcgctgacct cggtatagca ggtaaagata ctattttaga
     1381 aaatgagaca gatgtatatg aaatatatga tttaggtttt ggaaagtgta aatttgctgt
     1441 agcaggatta aagggagata gcatatatag agaagatgaa tatttaaaag tggcaacaaa
     1501 gtatcccaat atagcaaaaa agtattttaa agagaaaggt caaaaaattg aaataataaa
     1561 attaaatggt tctgtagaac tagctccaat agtagggttg tcagatgtaa ttgtagatat
     1621 agttgagact ggcaatacac ttaaagcaaa tggtcttgaa atattagaag acatatgtaa
     1681 tataagtgct agaattatat caaatagagc tagctataga tttaaatatg aacaaattca
     1741 gaatataatt cgtttatttg aagaacttga taattaaaat ataattgagc tttataagga
     1801 gggatttaag ttgagagaaa aagaaagcat aagagagtta aggggatatg aaccaaatca
     1861 cgtaaactgt aaagtaaaac ttgatgcaaa tgaaggaagt aagaggttgt ttaaatacct
     1921 tattaaagaa atttcagata gtgatataga tttaaattta tatcctgaag attcttatag
     1981 tgatttgaaa gaatctataa tagattacat aaatatttct ggagtaaata agaaaaatct
     2041 tctagtagga aacggttcaa gtgaaataat tgatttaata atacatactt ttgtagataa
     2101 agatgaagta atattgagtt tctctccaag ttttagtatg tattctatat atagtcagat
     2161 aaatgggtct aaatttatag gtgttgaaag tgatgaaaat ttagttataa atatagataa
     2221 tgtgattgaa aaagtaaagg agaataatcc taaaatagta attgtatgta atcctaataa
     2281 tccaacggga acaatattaa agagggaaga gataataaag cttttagatt caactaatag
     2341 tctagtggtt ttagatgaag catatatgga ttttggagaa gaaagcatgt taagtgatgt
     2401 ttttaagtat gataatctaa tagttcttag aactttatca aaggcatttg gattggctgg
     2461 aataagaaca ggatatatgt tatctaatag tagtttaata aattctgttg agaaagtaag
     2521 acctccatat aatttaaatt cattatcaga ctttattgct acaagagcac ttagaaataa
     2581 agatgtggta aaggcttata taaaggaagt aaaagaggaa agagaagttt tatatgaaga
     2641 aatgattggt atgggaatta aagcatacaa atcacaagca aattttatat tattttattc
     2701 tgaaatcgaa aatttatcac aaaaacttat tgatagaggt gttttaataa gaaaatttgg
     2761 tggaaagtta gaaaattatt acagagttac aattggtgat aaagaagaaa attctatgtt
     2821 tgttggagct ataagagata ttttgaaaaa ggagaaatag gtatgagaat ttggaaggta
     2881 gaaagaaata ctcttgaaac acagatttta gtagaattaa atattgatgg ttctggtaaa
     2941 gctgaaatag atactggaat aggattttta gaccatatgc ttaccttaat gtcatttcat
     3001 ggcaagttcg atttaaaagt gatttgtaag ggtgatacat atgtagatga ccaccatagt
     3061 gtagaagata taggtatagc tataggtgaa gcatttaaaa atgcactagg tgacaaaaaa
     3121 ggaatacgaa gatattctaa tatctatata cctatggatg aatctctatc aatggtagca
     3181 atagatataa gcaatagacc ttatcttgta tttaatgcta aatttgatac ccaaatgatt
     3241 ggtagtatga gtactcaatg ctttaaagaa ttttttagag cttttgtaaa tgaatcgaga
     3301 gtaacattac atatcaatct cttatatggg gaaaatgacc atcataagat agaaagtatt
     3361 tttaaagcat ttgcaagagc tttaaaagag ggtagtgaaa tagtttctaa tgagatagca
     3421 tcttcaaagg gggttttgta gaatgaatat aatagtagac tatggtcttg gaaatataga
     3481 ttctgtgtct aggggtttta gaaaagctgg aatagaaact aaaatttcta gtgatataga
     3541 tgaaataaaa caggcagatt cacttatact tccaggtgtt ggagctttta gagattctat
     3601 aagtgcctta gataaactag gattgatacc aataataaag gagcatgttt ctaagggtaa
     3661 atttatgata ggtatatgtc taggaatgca attactttat gaaaaaagtt atgagtatgg
     3721 agaatatgaa ggattaggac ttataaaagg aagtattgat aaactggata taagtttaaa
     3781 agtgccacat atgggatgga ataatctaaa atttaataag gcaaatgatg atatacttaa
     3841 atatataaat gaagatgatt atgtatactt cgttcactca tattatgcaa attcatcaaa
     3901 tgaagaacta attgcatttt cagaatatga aaagaaaatc cctgctattg ttagaaaagg
     3961 caatgtatat ggtatacaat ttcatcctga aaaaagtgga gaagtagggt taaatatatt
     4021 aagagcatat ggggagatga taaaatgata atatttccag caatagatat aaaagataat
     4081 aagtgtgtaa gactcacaca aggagaattt gacaaagtaa atgtatatta tgataatcca
     4141 ttagaagtag catataaatg gaaaaatgaa ggagcagaat atatacatat agtagattta
     4201 aatggtgcta gaagtgaatt tggtgtaaat acaaaaataa ttgaggacat agcaaacaat
     4261 atagatatac ctattcaagt tggcggtgga gttagggata aggaaaaagt caaaagttta
     4321 ataaatgctg gtgttactag agtaatatta ggcagtatag cgattgaaaa tttgaattta
     4381 gttgaagaat tggtcaatga atataaagag aaaattgtag tttctataga tgctaaagat
     4441 ggaaaagtag cagttagggg atgggaagtt gtaagcaatg tagactcatt aacattatgt
     4501 aaacaacttg aaaagatagg tgtacaaact attgtttata cagatatatc aaaagatggg
     4561 atgttgcaag gtccaaattt tgacatatat gaaagaatag caaaggaaac atcactaaat
     4621 gttattgctt ctggaggtgt aacttctata gaagacgtaa aaagactaaa agctatgaac
     4681 ttatatggtg caataatagg taaagcgctt tatgataaaa agatagactt taaggaggca
     4741 caacaattat gcttactagg agaataatac cttgtctaga tgtgagaaat ggaagagttg
     4801 taaaggggaa aaaatttaaa gatatagtag atgttgatag tccagaagta cttggaaaat
     4861 tttatagtga ttgtggtgca gatgaacttg tattctatga tataactgca tcaaatgaag
     4921 agagaaagac atctttagaa tttgtgacaa aagttgcaga aaatataaat ataccatttt
     4981 gtgttggtgg aggagtaaat aaattagaag atttcactga tattttaaga aaaggtgctg
     5041 ataaagtttc tataaattca tcagcagtca aaaatcctga attaataagg gaagcttcat
     5101 taaaatttgg agcacaatgt gttgttttat cgatagatgc aaagaaaaat gaagagggtt
     5161 catggagcgt atacgtaaaa ggtggaagag aaaaaactaa tctggatgca attgaatggg
     5221 ctgttaaagg tgtagaactt ggagcaggag aaatcgttgt aaatagtatg gatgaagatg
     5281 gaatgaaaaa tggatatgat atagaattat tatcaaagat aacttcttta gtaaatgtac
     5341 ctgtcatagc atctggaggc gctggaaaaa aggaagattt ttatgaggcg gttaataaat
     5401 ccaatgtaga tggaatttta gcagcatctg tttttcattt tggcgagata aaaataaacg
     5461 atttaaagaa atatttaaaa gacatgggtg tagaagtaag attataagtt aaatactaaa
     5521 aggagattag aaatatggat aataagtgta acaatgtata cagtgatgaa gtagagaaat
     5581 ttataagaag tataaagttt gatgataaag ggttagtccc tgttgttgtt caagaggtag
     5641 ttagcaaaga tgtactgatg ttagcatata tgaataaaga tgctataaag aaaaccttaa
     5701 aagataaagt agcttgctat tttagtagaa gtagacaaga actttgggtt aaaggtgaga
     5761 catctggtaa tactcaaaaa gtagttaaga tgtcttatga ttgtgatgta gatactatac
     5821 tactttttgt tgaacaaact ggtgtagctt gtcatacagg caattattct tgtttttaca
     5881 gagacttgtt tgatgatagt tcaaaaatgg agcttgaagt acaaacaaat atattgaaag
     5941 aattatatga tttaatcaat gaaagaaaaa ataatccagt tgaaggttct tatactaatt
     6001 atctttttga aaaaggaata gataagattc ttaaaaaagt aggtgaagag agtagtgagg
     6061 taattatagc atcaaaaaat actgataaaa gtgaacttat ttacgaaatt agcgatttag
     6121 tatatcatac attggtttta atgatagaaa aaggtgttga aattgatgaa ataaaaaaag
     6181 aattacttaa aagaagaaaa tagaataaga tagattaatg ggtaataaga aaatatacca
     6241 cttttaataa aaagtggtat attttttatt ttatattttg atttatagaa taatatttaa
     6301 attacttgat aaaattaact atatatataa tttattgata aaata
//
LOCUS       DAKBNN010000209         6323 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000209 DAKBNN010000000
VERSION     DAKBNN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.215"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            465..1277
                     /gene="modA"
                     /locus_tag="OUB02_003331"
     CDS             465..1277
                     /gene="modA"
                     /locus_tag="OUB02_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8887059.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1287..2078
                     /locus_tag="OUB02_003332"
     CDS             1287..2078
                     /locus_tag="OUB02_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887060.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            2080..2775
                     /locus_tag="OUB02_003333"
     CDS             2080..2775
                     /locus_tag="OUB02_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8887061.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2762..3493
                     /locus_tag="OUB02_003334"
     CDS             2762..3493
                     /locus_tag="OUB02_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887062.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3807..4607
                     /locus_tag="OUB02_003335"
     CDS             3807..4607
                     /locus_tag="OUB02_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCT8887063.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            5146..5862
                     /locus_tag="OUB02_003336"
     CDS             5146..5862
                     /locus_tag="OUB02_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887064.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2478 a          760 c          867 g         2218 t
ORIGIN      
        1 ctacaagtac tcttttaatt agaatatata gtaatataat aattttagtt gtggcacatc
       61 aaaattttca actatctcat aaatacatac tctttctttt gtattcaatt ctgatataat
      121 acttttagta atttgttaat aaaaagcaat taaaacatat ctatatgttt caatgttcta
      181 ttttttcact gaaatataat aaatataaaa ttagttaaaa agtaaaaaat ctacaataag
      241 ttaatttgat ttaagttttt aaatttaaaa tactttaaag tattaataaa aattaaaaat
      301 aaaataacta cctatggaaa tgacattatg aatagatttt ttcttaacta tacataacaa
      361 aacataacaa aacattataa aacacaacaa aaacttagac atcgttttct tagtatgata
      421 tactaaaaaa gaatttacac taatttaata agggggaata tcttatgttt aaaaaacttt
      481 cagtactagc tttagctatg attatttcat taagtattac tgcatgtaat tctaaagaaa
      541 acaaagctaa tgatgaatct aatactaaag tgacaacagc tgatgattca aaagaaaaag
      601 agactctttt tgtctattca ggggcaggtt taaagaaacc aatggatgaa atagcaaaaa
      661 aatttgagaa agaaaataat gtcaaagttg aatatagcta tgcaggttct gcacagctaa
      721 tagctcaaat tgagacaagt cacaaaggag acgtatttat tgttggttct gaaccaattt
      781 attcaaaggc ctttgaaaaa gaacttgtag gtgaacataa aacggttgca catcatacac
      841 ctgcaatagt tgtacctaaa ggaaatccag ctaatattca aaaattggaa gacttaaata
      901 aagatggagt taaacttatt ttgggagaca aagagtctaa tgctataggt aagactacac
      961 aaaaaatact tgaaaaaaat aatttaaaat ctataaatga taatgttgtc tctacagctg
     1021 caactgtaaa tgaaatgatt gtacagttaa catcaagtaa agctgatgct acaatagcca
     1081 caaaagattc ggtctttggt aatgaagatg tagaagttat agaaattgcc caagataaaa
     1141 atatagacca actcatatca gcaggaatcg taaactatag tgataaaaaa gatttagcta
     1201 ataaatttat aaattatata gagtcagacg aagctaaaca gatatttaaa aaatatggtt
     1261 ttgaacctgt taagtagtga ttaaacttga aaaataaatt ttttactact ctacttactg
     1321 gattttttgc tatagtcttt ttgtttattg tatcaccact tcttatgtta atcattaaag
     1381 ggataagtta tgttcctata tgcttaaaat cagtggaagt acagtatgca ataacactta
     1441 gtattaaaac atctttaata tctactatag tatgcctttt attagctatt ccagtagcat
     1501 attttttaca tattactaaa ttaccattta aaaaactgat tatacagata ataaatctcc
     1561 ctatgtctct tccacactta gtatcaggaa ttgctctttt acttttgttt ggaagaatgg
     1621 ggattggaga ttccatttat aagattttta agcttgattt tatatttaca aagcaaggta
     1681 tagttttagc acaaatattt gtaaatcttc cacttactat taaaatactt cacacttcat
     1741 taaatgaaag taatgaaaag atgatatttg ttgcacgtac ccttggatgt aatagttggg
     1801 aagcatttag atttataatt cttccaaatc taaaaactgg aatcatatct gcaactgtaa
     1861 tgacttggtc aagagcactt ggtgaatttg gtgcagtagc tatgatagca ggttctacac
     1921 gtatgaaaac tgaaattatt cctacatcta tatatttaaa tatgtctact ggtgatatag
     1981 acattgctat cggaatagct gtaattttga tatttctctc acttacatgc ttgatgcttt
     2041 ttgagatatt ttttaataga gaggttgata agaattaata tgttaaaaat aaaaaattta
     2101 tctcttgatt tacctaattt taaattacaa gaaaatagtt taaatattaa taatcatgaa
     2161 tactttgttt tattaggagc tagtgggtca ggtaaaactc tatttctaga aacattagca
     2221 ggaagatatc catctgctaa aggaaatgta tcatacaaag aaaatttaat atcaaactta
     2281 cctccagaaa atagaaatat aggatttgta tatcaaaatt ttgaattatt tccaaatatg
     2341 aatgttgaag ataatataag gtttccactt aaattgagaa aaatacctat agatgaacag
     2401 attcttaagg ctaaccactt gtctaaatta atgcagatag aagatataaa aaataggtat
     2461 cctaaaaatt taagtggagg cgaaaagcaa cgagttgctt ttgcacgtgc cttaatagct
     2521 gaacctgata tattactttt agatgaacct atgagttcat tggattatat tacaaaaaaa
     2581 catataagca aaattttgaa ggatgtctac ataaaataca aaccaacagt gattcatgtt
     2641 actcatgata tttctgaagc aatgtttttt gctcataaga taggaataat gaacaatgga
     2701 aaaataaatc atatttttga tttaaatgaa gaaataaaaa ataaaggaga atcttttttc
     2761 tatgaatata tttgatattt tgagagatta tctattagta caagtcgata aatataattt
     2821 aaatatggat atgataagta ttgtgtccaa atctctatct tcaaaagaag caattggaaa
     2881 tactaagcgt aaagatttcc caattatagt tggtaaagaa ataatgcttg aagctgactt
     2941 taaaggagct aaaggacaag catttacttc tactccatct acatttgaag gttctttaaa
     3001 agacatatta tcattagatt tacatgacaa ttctcatgat agaagtcttt ttatagcatc
     3061 attaaatgca gttatgaaat acttaggcaa gacggataga accattcatt gtaaaaataa
     3121 cgagccagag gtatgtgcaa aaaaattccc tgagtttata aaaatggaat ttggtaatcc
     3181 aaaagtagca attataggat atcaaccagc cataattgat aatattaagg actttttcga
     3241 aacaagagta ttagatttaa atcctgaatt tgtggatact attcaatata atgttaagat
     3301 tgaagatggt atacgagatt atgaagatgt aatatcttgg gctgatttag tgatttgcac
     3361 aggaagtaca ttatgtaata actcaattat aaattttcta tcacttaaca aaccagttta
     3421 ctattatgga actacgatag ctggtgcatc aaatatttta ggtttaaaaa gactctgttt
     3481 ttgtagtaaa taagcaatca tatcatcaag gagtacacaa atatatatgt gtactcttta
     3541 aatttaaaaa gttattactt ttattctaag tactaaagta aaattctatt aaaatgaatc
     3601 ccgaataaat gtacaatccc tttaaatctc aattcctatc aatcattaat attttgatta
     3661 gtcttataca taaatgtttt ctttatttta aactaataaa aaaattttat tccattaatc
     3721 tactctatat ttttcagcat ttaagaaata atttttagta ctttgtttga taatataact
     3781 aagacaacct ttcaggagat gattaaatga catttaagaa taaaaaattt actataaata
     3841 aatataaaat tctatctatt attctagttt tatgtttgat tttaaccgct tttagtagct
     3901 atgtgaatta tagtcataaa aaatttaaag attatgttgt cttctatgtt cagcatcaag
     3961 atgatgaagt gttgtgggct gggagtgcta ttgttaatgc tataaaagaa agaggaaaaa
     4021 atcatgtatt tgtggtttta gtatctactg gatgtggtat atccgtcttt gataaatata
     4081 aaaaatatga aaacctaaca aatatacaaa aatcagaata tagaaataga gaatttttag
     4141 cttctcttaa tagccttggt gtaataaggg aaaatataat attattacca gaaacaaata
     4201 caaatggtaa tactgacttc aattacatgg aaaaaattgc tcttacattt gaagaaaaat
     4261 ttaaaaatgt aactcatata gcacatactt ataaattaga tgaccattta caacatttaa
     4321 aaaatggttc tgttatccaa aatttatata atgctggtaa aataaaagat gctaaatatt
     4381 ttgtaaaacc taaatttgct gataaaataa cttttaataa taagatagtt tataaagctc
     4441 attctactga ggactatgaa aaggttaaaa atgcctgtca acaatacaaa attgttgatg
     4501 aattagaata tagagaaggt attggctata agtctgacca taaatcattt gataagttat
     4561 taaataataa agatgttcct gctatacttc atacacctaa tttataagaa atatttattc
     4621 tttaatttaa gttataaaaa ggaaattatt tgagattttg tgatatagca ctttttgtgc
     4681 tatactactt tttctcatac aactattacc catatctaat ttcatcatct cttaaaatat
     4741 ataaagatat catcattaaa acctataagc cttattaaat gctttataca tacctacatt
     4801 aaactacctg tttatattaa actacattaa aataaatatt tgttataaat tcttaataat
     4861 tatgaaactc atttgttatt tatatttgtt aatatatgtt taaacctaaa aaattaataa
     4921 accaaaataa cataagaagt gtgatagatt accctagata ttttcataaa cttttacctt
     4981 cagattaata tgggcgtcta tacttttaaa tttactatat gcattgacaa tatattaaaa
     5041 gtactagttt ttatttcata atgaacgggc ttactgtttt ctaatacctc tttttggtat
     5101 aaaataaaaa ttggttaaaa ataaaatttc aagggggtta ataaattgaa tcaaaatata
     5161 ttggttgtag atgatgatag agagatagtt gatgctatag agttctattt gagacctgat
     5221 aactttaatg ttttaaaggc atatgatgga cttgaagcat tggacatact aatcgaaaat
     5281 gatattaaat taataataat ggatgtaatg atgcccaatt tagatgggct taaaactaca
     5341 cttaaaataa gagaaaaaag gaatatacca ataattctct tatctgcaaa atcagaagat
     5401 atggacaaaa ttataggtct taatatgggt gctgatgact atataactaa accatttaat
     5461 ccactagaac ttactgctcg agtaaaatct caacttagaa ggtatataaa tttaggtgat
     5521 tttagtaata caaataactc aaatgatact aattatattc taaaaagtgg tggcctagag
     5581 ctaaatacag atacaaaaat agtttcttta gatggtgagg aaattaaaat gacaccactt
     5641 gaatataaaa tacttacact tcttatgtca cgaaaaggta aaattttctc ttctcatgag
     5701 atttatgaaa atgtctggaa tgaggatgct tatagttgtg agagaactat tgctgttcat
     5761 attagaagaa ttagagaaaa gattgaaatt aatccaaaag aaccaaaata tttaaaggtg
     5821 gtgtggggca ttggttacaa aattgaaaaa cttaattatt aatgattcta aagcaaaatt
     5881 tattttatta ttattactgt ttctattagt ttctatatta tttgaagcag tttatgattt
     5941 gaataataac ttttctaata ttaattatta ttatagagag tttgctctcc ctcattttta
     6001 ttttttcggt actcctgtac atttgtcaaa agtagaatta gagtttgtct gcataggtat
     6061 tatattatca agttttttat tgactatact atgttttaca ttgtataaaa aaaataaaca
     6121 aactaaagtc aaattcttag agaatgtcat aaaccttttt tctaaggtaa aaatagaact
     6181 tacacttata ttaactttga tactattaac cttaaggttt catgcattta caagtaataa
     6241 aatgttttac aacactaatt taataatcta caattttata attatatcta tagtctatat
     6301 aatatatgaa aatattaaat ata
//
LOCUS       DAKBNN010000210         6258 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000210 DAKBNN010000000
VERSION     DAKBNN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.216"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            144..689
                     /locus_tag="OUB02_003337"
     CDS             144..689
                     /locus_tag="OUB02_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCT8887065.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            702..1715
                     /locus_tag="OUB02_003338"
     CDS             702..1715
                     /locus_tag="OUB02_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCT8887066.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1937..3910
                     /locus_tag="OUB02_003339"
     CDS             1937..3910
                     /locus_tag="OUB02_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCT8887067.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEICVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECILNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3979..4338
                     /locus_tag="OUB02_003340"
     CDS             3979..4338
                     /locus_tag="OUB02_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887068.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4373..4729
                     /locus_tag="OUB02_003341"
     CDS             4373..4729
                     /locus_tag="OUB02_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCT8887069.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4766..6058
                     /locus_tag="OUB02_003342"
     CDS             4766..6058
                     /locus_tag="OUB02_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCT8887070.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2478 a          584 c         1183 g         2013 t
ORIGIN      
        1 tcaaaaatat tatattaaaa tgatattaat aaaaaaacat ttacaatatt agcatatagt
       61 acagaaaaaa gatgtaattt gtaaaattta tggtaagcag taaaattaaa aatttaaaga
      121 tattataggg aggttataat tttatggaaa acgttattac aggtctaagt aaaggtgcag
      181 agtggtttat cggtttgttt cagaaagggg gagaacaatt tataggtctt gtttcaggta
      241 ctcttccaac gctaattgta ttaatggtag ctattaactc tttgattaag ataattggtg
      301 aggaaagagt taataattgg gcttctaagc taggaaagaa ctcttttaca agatatattc
      361 tacttccatt agtatcagta tttttcttgg gaaatcctat gtgctataca tttggtagat
      421 ttttaaaaga agagcacaaa gcaggatttt atgatgcagc agtttcattt gtacatccaa
      481 ttacaggtct ttttccacat gcaaatgcag gagaactttt tgtatggttg ggaatatctg
      541 caggtttgac tactttaggt aaagaaacta ctacattggc attgtggtat tttatagttg
      601 gtctaattgt aatttttata agaggtatag taacagaaaa aatgtatgct tttctaacaa
      661 gaaaaaatag agcaacaaat aaggcttagg aggtagataa aatggaggag aataaaatat
      721 taaaaataga aaagggaact tcaggctggg gaggtccttt gtatataaaa aaagaaggga
      781 atagaaataa gatactatca atgactgctg gtggtatcca tgaagtaaca ttaaaaataa
      841 aagaactact gggttgtgaa attgtagatg gatttaaaac tggagtttct gatgaagaag
      901 tagcagtagt tattattgat tgtggtggaa ctgctagatg tggagtttat ccaaagaaaa
      961 aaatacctac aataaatgta aatccagttg gaaaaacagg tcctttagca aaatttataa
     1021 cagaagaata ttatgtatca gatgttaatc ctaactgtat aagtgttgta gatggggaag
     1081 atatgccaca aaaatctcaa gaaaataaaa gcgaaaataa gagttctatt agaaagcctg
     1141 ataattatga tgaggttaag tcaaaagcac aaggtgaata tgctaaaaag aacataatac
     1201 tttctattgg tcaaggggca gggcaagtag tatcaaaatt ttatgatgca ggtcgagata
     1261 ctatccaaat ggtaatgaat aacgtaatac catttatggc atttgtaagt atgcttatgg
     1321 gaattatttt agcatctgga ctgggagact ggattgcaag agttatatct ccacttgcag
     1381 gtaatattgg tggattattg attatatcag taatttgcac acttccattc ttatcaccaa
     1441 tactaggacc tggtgcagtt atagctcaag tagtagggac tcttgtaggt acacaaattg
     1501 gtcttggtgc aataccagca tatttagcac ttccagcttt atttgcaata aatgggcaag
     1561 ctggatgtga ttttgtacca gtaggattaa gtttgggaga agcagaacca gaaactgtag
     1621 agtatggtgt tccagcttta ttttactctc gtttaataac tggtccaata gctgttatta
     1681 ttgcatatgg agttgcagta tttgcattga gataatattt aatgtcaata taaggcttgg
     1741 aattaattaa taaaattgta aatataaatt taagctagtc aagatttttt gttaatgaaa
     1801 gactagcttt tatactatta attagctgta attatcgagt tagattaata ctaaattttc
     1861 ctttattaaa gtgaaatttt atgtattaat ttggtttcaa atttgtacta gaatgaaatc
     1921 aaggaggaga tacaaaatga gtgtaactga gaaaattcct tctattgacc gcaagatatt
     1981 taagatattg attatgtgta gtaaaaaaga gtttgtgagt attaactcaa tagcaaatga
     2041 attgaatgtc acaactagaa gtgtaaggac gtatataaaa caattgaata aagatttagg
     2101 taatgatata gcagtaatca aatatataaa gggtcaaggc tataaactag aaataaaaga
     2161 tgagcagata ctcaataaga ttattgatat gaatagaaaa aatgtatttt cattaaattc
     2221 aaaagaagat agagtagagt ttatactaaa ttacttgata gaactagatg gatttattac
     2281 acttgatagt ttagctgatg agatatgtgt aggaagaact actttagtaa atgatttcca
     2341 gtatgtagaa aaagttttgg cttcatataa tttaaacttg attaaaaaac agaatacagg
     2401 tatgaaatta aatggaaatg aacttgatat tagactgttt atattaaatc agttgtataa
     2461 aaactcaaga aaagatttca gtaattcaaa gtattttaaa ggaatcaaaa aagaagaaat
     2521 cataaattta gaagaaaaac tattgacttt atttaaaaaa aataattttt atgttacaga
     2581 tgaaatgtta agagaagtta ttaactacat agtagtttta gtctatagag taaaagaaga
     2641 aaagaaagta aaagattatg atgtaaagtt tgatttactt aaatcatatg atgaatactt
     2701 tattgctagg gaaataaaac atataatatc aggaatgttt gaatgtattt taaatgatga
     2761 agaaattatt tatttaacta taccgttagt tagtggaaat gcaccagcat cagaatgtgc
     2821 tttaaatagt agtagaatta gtaaaaacat tgatgagttg atggaaaata tatttaatca
     2881 aatatatgtc gatatgggta tttttataaa tgaggatgaa ttaagagtag gtctaggata
     2941 tcatcttagt tttactttaa acaggctatt atttaatata aagttaaaaa atgttttgtt
     3001 agaagagatt aagcaaaatt atatacttcc atttaaatta gcacaaatag caggaaaagt
     3061 aatagagaaa aagtataact tagaagtttc agaagatgag attggatata tagcaatcca
     3121 tttcagtgga tatttagaaa gaaatagcag tagattctat tcaataaaaa aaatagcaat
     3181 tatatgtagt acaggtctag gaacagcaaa actattgaaa ataagagtag aaaaactgat
     3241 aggaaataat cctaaaattg atactctttc aagttttaat ttgagaaata ttaacttaga
     3301 tgaatacgat atagttttta caactattga tttagacaca tcaaatataa atacaatagt
     3361 attaaaaata aatacaatat ttgatgaaaa caaactaaga gaacaattaa aaacagtatt
     3421 gtgcctaaga gaagggaata ttgacactac taattctaca aatttactaa tcaataattt
     3481 gttagatgaa gataaattta tgatattaaa tgaaaagact atacttgcct cattagaaaa
     3541 aatgatggac catcttatgg atttgggatg tattgatgag aagtttagaa aaaatatagt
     3601 caatagagaa gaaaaatctc ccacagtatt tgataaaggt ttattatttc ctcattctgt
     3661 aaatgaaaaa tcagataagt ttttaatggc agttggaatt ttagaagaac caatagaata
     3721 tgcaaataga agtataaaaa taattcttat gacaatgttt ccatgtgaga ataagataga
     3781 ctcagactta ttagtaaaaa tttatgaaga agttttaaag ataggccaag atgtaaaatt
     3841 aacgaataag ataagtaaat gtagaacttt tttagaattt aaaaaggtgt tacttaaaaa
     3901 ccttatataa ctattgtaca gcatttatag ttgttataca gatttaaaaa taatgtgatt
     3961 tttggaaggt gtataaatat gaaattatta gtaattttat ttgttgctta tattttaaat
     4021 actatattta taatgaagca gaataaattg tattttaaaa ctttaaatag agctaaaaaa
     4081 aagggtgata tagtgtctac aggcaaaaag aaaagctatt tttctaaagg aagtatagcc
     4141 gttatatctt cagattcaga tgggtttata aagtttggag aaattttaaa agggcgtaca
     4201 gtaatggcta aatttaaaga gattgaagaa atagaaggat tagatatata tagtgcagag
     4261 caaaaattta aagatgaaga gagtattgta caagctataa aatttataaa agaaaaaata
     4321 aatgtttctt ttaattaact caatataaat ataaattatc aggaggaatg tcatgaaata
     4381 tgaagttaag ataagtggga taggcagttt agttaaagag ttaatggatg aaagcaattg
     4441 tcttattata tatgatgaaa ccattaatga tgacgactta aaggatatat ctgtaattca
     4501 ttcaatagca acgttaaaaa gtgatgttga aataggagat acacttacta tagggaatag
     4561 agattattgt atagtttcag ttggtgatat tgctcagaag actcttagag aaattgggca
     4621 ttgtacaatc aaatttgatg gaaaatgtga agtaaattta ccaggagaaa tacatgtgga
     4681 aataggaaat ccagatataa ctattggaga tttaataact atttcttaat ttgaaaataa
     4741 aattattata acaggaggaa ttggtatgtt agatttaaac agaaaacttg caaaattgga
     4801 agaggaaggt aaaaaaataa aagtagctct tataggagct gggcatatgg gaaatggaat
     4861 ggtaagccaa atggcaaata tgaaaggtat tgaagcttct attgttgtag atataaattt
     4921 ggagttggct cataaagctt ttactgatgc aggaatagat gaagaaatta tagataatgt
     4981 aacaaatgaa aaggatgcag aaataaaatt acaagaagga aaagtattga catgtaatga
     5041 ctttcttgtg gcttgtaaaa ctaagtcaat agatgtagtt atagatgcaa ctgggggtat
     5101 tgcaattggt gcagagatag cattaaactc tattttaaat aaaaaacata tagttatgtt
     5161 aaatgctgaa actgattgtg ttgttggacc aatacttaaa aaattagcag atgatgctgg
     5221 agtaatattt acaggttctg caggagatga gccaggtgca gtaatggaat tatttgattt
     5281 tgcagatgca atggggtttg aagttagagt gataggtaaa ggtaaaaata ataaattaga
     5341 tttagattgt aatcctgata cagttagaga agaagcagaa agaaaaggag cttcacctca
     5401 tatgatagct tcttttaaag aaggaactaa aacaatggta gaaatggctt taatgtgtaa
     5461 tgcaactgga tttgtgcctg atgtaagagg tggtcatggt attgaagcta cagttaatga
     5521 agtacctaag aaatatgcat taaaaagcga aggtggagta ttagataatt acggtgtagt
     5581 tgattttgta aatggtatag cacctggagt tttcgtagta gtagcccata aattaaaagc
     5641 agttaatgat gaactaaaat atttaagtat gggtgatgga cctaattaca tattatatag
     5701 accatatcat ttatgtagtt tagagactcc tttatcagca gctatggcag ttttagacca
     5761 taaggctaca atagtaccta aagctggact agtagcagaa gttatgacta tagctaagaa
     5821 agatttgaaa aaaggtgagt ttatggatgg ctatggagca tatacatgct atggtactat
     5881 agaaaaatac gatgtagcaa aggctatgaa tgctgtacct attggtttga taagtaaaaa
     5941 gactaaagtt gtaaaagata ttaaaaaagg tgaagttata acttacgata tggtagagat
     6001 agaaaaagat actactttat atcatttaag acaattgcaa gaaaaaatat ttgggtaaga
     6061 tttgatggta atataaatag atattagtaa aaagggttga tggttatttt taaatcaacc
     6121 cttttaaatt tttatatatt cttgttagga tactttcttt aaaagtatat tataggttat
     6181 ttgtagtcgt aaaaattatt ttgaattagt tctgcaattt tatattcaca ttgaaataat
     6241 attattaaaa attttaat
//
LOCUS       DAKBNN010000211         6246 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000211 DAKBNN010000000
VERSION     DAKBNN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.217"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..640
                     /locus_tag="OUB02_003343"
     CDS             <1..640
                     /locus_tag="OUB02_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887071.1"
                     /translation="NKLKEVTKNNEEIVPKNLEGLSIEQLIEIRDKAIDKNLEGLREF
                     GEDSVKKRKDSILNFEFGLVAFVNKNYSNLTEKEKLIRQINIVNYENSINDIMDPSGS
                     FFELANQNYQVNEFLLKNGKDYKEWSYEGLDFLIDHLEELCKFVDFDVKDVIDIIDPT
                     IKTDLSEEQKKSLNDKLKKISSSDTLSEEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(797..1003)
                     /locus_tag="OUB02_003344"
     CDS             complement(797..1003)
                     /locus_tag="OUB02_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887072.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(1406..1801)
                     /locus_tag="OUB02_003345"
     CDS             complement(1406..1801)
                     /locus_tag="OUB02_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCT8887073.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            1942..2334
                     /locus_tag="OUB02_003346"
     CDS             1942..2334
                     /locus_tag="OUB02_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887074.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(2434..2850)
                     /locus_tag="OUB02_003347"
     CDS             complement(2434..2850)
                     /locus_tag="OUB02_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HCT8887075.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(2865..3776)
                     /locus_tag="OUB02_003348"
     CDS             complement(2865..3776)
                     /locus_tag="OUB02_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8887076.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(3958..4980)
                     /gene="splB"
                     /locus_tag="OUB02_003349"
     CDS             complement(3958..4980)
                     /gene="splB"
                     /locus_tag="OUB02_003349"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HCT8887077.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(5320..6222)
                     /locus_tag="OUB02_003350"
     CDS             complement(5320..6222)
                     /locus_tag="OUB02_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT8887078.1"
                     /translation="MKKSTQKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDK
                     GFSYNTINNHKRSLKASFYIAIQDDCVRKNPFDFKLSEVIENDTKEKVALTEEQEQAL
                     LSFIKTDNVYHKYYDDVLILLKTGLRISELCGLTIMDIDFIHEVVVINHQLLKSKEQG
                     YYIETPKTKSGTRQVPLSRETIQAFQRVIKKRPKAEPFVIDGRGNFLFVNHKGKPKVA
                     IDYNMLFVRIVKKYNKHHKDNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNI
                     SITMNWYAHASIDTAKSEVQRLIA"
BASE COUNT         2075 a         1021 c          788 g         2362 t
ORIGIN      
        1 caataaattg aaagaagtta ctaagaataa tgaagaaata gtacctaaaa acttagaagg
       61 tttaagtata gaacaattaa tagagattag agataaagcc attgataaaa atttagaagg
      121 tcttagggaa tttggagaag attcagtaaa aaaacgtaaa gattcaattt taaactttga
      181 atttggatta gtagcttttg ttaacaagaa ttatagcaac ttaacagaaa aagagaaatt
      241 aataagacaa attaatattg ttaactatga aaactctata aatgatataa tggatccttc
      301 aggttcattc tttgaattag caaatcagaa ttaccaagtg aatgagtttc tattaaaaaa
      361 cggaaaagat tataaagaat ggtcatatga aggtctagat tttctaattg atcatctaga
      421 agaactgtgc aaatttgttg attttgatgt aaaagatgta atagatatta ttgatcctac
      481 aataaaaaca gacctttcag aggaacaaaa gaaatctttg aatgataaat taaagaaaat
      541 atcttcaagt gatactttaa gtgaagaagt taaaaaggag ataaaaaact gggaaaacaa
      601 tttaaataat ttaaatatga ataaaaataa ttctaattaa ttttctcata agaaaaatgc
      661 ttatatcgtg ataatgtgaa atgtacataa ttataaacaa aaaaggggct gtgacactaa
      721 aaaatagtgc tacgcccttt tttatttgta ataatcatca atacaaatta ttttattata
      781 tgtaattatt tatgtcttaa ccaattttat cgaaaataac agtcttctct tttaaagaga
      841 tttttcctac ttgatatcca tattcattta attccttttt atatttcaaa aacgaatggt
      901 ctatctctat cccagcaata tcttttattt cttcaaaagt taatttaaac gattgactat
      961 tgtttttctt aacatattcc cataaagaac tatatttact catcttatat cactcctttt
     1021 aaaatcatat cttatctatt aatttatatt ctatcattat taatgtataa agtcatgcta
     1081 cttattattt atatataaaa atatccaaat aataagtagc aattttttat agtcataata
     1141 tttttttcag attaaataaa aatcaattat agttaaaata ttttttaaaa gaaatctatc
     1201 ccattataaa gtaaatttta attatcttat ttctagtatg taattattca aaatagtata
     1261 tcattattga aatacacatc taataactaa aaatttttac atattcatta tttaaaaatc
     1321 tttttattgc atttaaatta aatctatcaa catttcagta ataacgtttt cttaattttc
     1381 actatggatg tcgattcaat tataattaaa gctctacatc aatcttttta ccactcattg
     1441 aacaacaact aacttttgcc ttatcaatag agaatatttc aaacttacca tctcctacag
     1501 agtacatctg acctaaactt ggcattattt caagtgcctt agcttgtgtt tcttttgatg
     1561 taacaaatac tgcttcacct aaaatacgta gagtattaag gttttggtct atacaacaaa
     1621 gttcaacctt tggattaaaa ataagttgct taaacatatc cttttgatta gatgtgcaaa
     1681 atgtaagttt accattgtat tccataacaa atcctaaagg acgcacatgt gcttgattgt
     1741 tctcatcaac tgttgctaaa taataaactc gtgccttgtt caaaaattct aatgctttca
     1801 taattcttac acccctttta aatacttgta ttaaggatta tcctttgata taattttaat
     1861 ataaaataca gaaaaaacaa gtatgcagtt ttttctgata aagtataaaa aagatactac
     1921 tataactaag gagtaattaa aatgagtata aatactgaaa aaaatataac taaaaacgtt
     1981 gaagaattaa cctgtccaat ccgttatgca ttagacattg tcggtggtaa gtggaaatta
     2041 cctattatat gtatgttagc agttgaaaat ccaattagat atagtagcat aaaaagaaaa
     2101 ttggatggca ttacaaatac tatgctagct caatcattaa aagatttaga gtctactggt
     2161 attgttcatc gtaaacaata taatgaaatt cctccaaaag ttgaatatac attaacaagt
     2221 aaaggtaaaa gtattgtgcc tattttacaa caatttgcta attggggtgc tactaatatg
     2281 caagaaaaaa acacatgtgg acttagttgc aaagaatgta gaaagataaa gtaaatttaa
     2341 atttactaaa ttattcatat tatagactat atattaattt atctgattga atataaaaac
     2401 aggatgactt atatattcat cctgttctat atgttaagct ctaaatttaa ttagttcaag
     2461 catatcgttt aataccaata aactatctac ttgaatcatt agccatttac ttccattaaa
     2521 aaagtttacc tcattatata tttttttgat ataatcacaa caagtattaa taaataaatc
     2581 aacctctact ctattgcttt cacttacaat aaccaaagct ttaaaataac cttcttgtgg
     2641 atataaagta catagtgatt tacctctttt tttatatttt acattccaac ctctttgcat
     2701 tgaacattta ctgtattcaa gcttaggttc tacattataa gtagaaacca aagttttatt
     2761 taacttgtcc cataatggat tattaataaa ttcattaatt tgctcaaatg taggtttagt
     2821 atcttgaaaa aacgcatctt gccaattcat attcatcatc ctttctattg ttcaatagga
     2881 accggaacaa tccaatgttc cagatatgct gcttcaggtg tagtggggta atacatctca
     2941 attgcaaaat cagtagttgt taaattatgt ttcttcatcc atctttccat aaatttatct
     3001 gctttaaaaa cagctgaatt aactaactca ttaaagtttt ctgcctcaaa tccacatact
     3061 aaatattctt tagctggcaa ctcaaaagta ctatatcctt ctactgatgt acctgactct
     3121 acctctacac ctgccatata catacaattt ccttcttttg catttcccat ataaagagca
     3181 gcaaattcat ttccacctgg aatttgattt ggtatattat gtctttgact tccaagtttc
     3241 atccataatt cctctactat tgcaattcca gttgtttcac cgcccaccaa ctggcaaata
     3301 ggtacttcta tttctattcc aacaaatgtc cttggattac tcaatgtctt acgattgact
     3361 tcaatcacta tatcatctgc aatcagtgga acaccttcat caaccatagc ataatttaaa
     3421 attaattctg gcttaataaa gtgattgagt tttactggat ttgctctata tttttctgga
     3481 gttaatccat atgctttttt aaaggaacgt gtaaatgtct catgactagc aaatccaaaa
     3541 tttaaagcta catcaagtat acgattctta tgttctgcta agtattcaga agctcttgca
     3601 agacgacgaa gctttatata ctccattaca gtcttattta ccaatctttt aaatagtctt
     3661 tgatagtaaa atggtgataa atttgcttta tttgctaatt cattaattga tatttcttct
     3721 gataaatttg cttctattaa atccaatgta atttgaatag actcccatgc atgcatacta
     3781 taactcctcc tgatattttt gttactctta tcctttatag tatcaaaact agaaaaaatg
     3841 ttcttgatag atagtgccct ttttaatttt cttatatgat agtgctaaat ctcattgctt
     3901 tattataaac ataaaatata taaaaataaa atatagcaaa tctttaggta agatttgcta
     3961 tattatatat tttattaaac tatttttaaa tactccattt atttccttaa gaaagaactc
     4021 tttcatatca tctaattgtt ctttatcata cacgtactta ccatatccaa attgaccgta
     4081 tttaaacttt ctatcttcat tttccattgg caatagtgta tctggataaa tctctagtat
     4141 tctatttttt gctttagttg tatatctatg agatataact tcaaatataa tttttttatc
     4201 aaaatccttt ggtaaagctt cttttatact tataatcaga tttttatact cttctttcca
     4261 accttcatat aaaaacacag gtgctattat gaaaccaatc tgataaccag ccctagcaag
     4321 ttttacagct gactctattc ttttatttgc agaggctgta taatgctcat gttcatttat
     4381 aattctagga gtattgatac taaatcgtat ttctgtatga ttattatgtt cagcatccaa
     4441 taagctatcc acatcactgt atttagtaac aaatctaaat cttccttttt cctctcttcc
     4501 aaaaaactct attgttttct tcaaagaatt agtatatggc tctactggaa ctgggtctga
     4561 tgtagctgcc ccttcaaata tggttacctg tggctttcta tcttcaatat atttttttgt
     4621 ctgttcaagt atttcatcta catttacaaa tacccttata aatggtttat cacctaattg
     4681 tgtgttcaaa taacaatact cacatcttcc catacatcca ctgacaagtg gtagttgata
     4741 attagcagaa ggtttacatg tttgaaactt cagacttttt ctaatcccaa ctaccatagt
     4801 ctgcttccca cttctatatt gttcatatag attttcacct ggaatatgtt ctttaatcct
     4861 atttgatgat atattaatta tctctacatc tttattatct ctaaatttat tgtatatttt
     4921 tttacccaca tcagtgtcta aagttccttt ttcgaatatt attcttttgg gattaaacat
     4981 ttatttcacc tctacttaaa gtttttaaat cgaagaattt tataaaatat tacttaatta
     5041 atcttccctt ttttaaactc ctttatttat tagaaagtaa aataaaagct taagacaaat
     5101 ttattatata tataacttct atacacaatt tttttactat taattactta taaattatat
     5161 atgtcttttt gactatcttt ttatatcttc ttaaatgtcc gcaaagcctt attttatcgg
     5221 ctctacgggc attttgcttt tgtggtaaac ctcacatatc taggtctatc ttcttatatt
     5281 ttcgctatca agcgtggtta aaatcgtggt aaatacttct tatgcaatta gacgctgaac
     5341 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatgtt
     5401 tgaatgtccc atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccaatcttgt
     5461 gcagaatgta tggcgtagcg tatgtggtgt gatatgtggc aaagggttat ccttatggtg
     5521 cttgttgtat ttctttacta tacggacaaa aagcatattg taatcaatcg caactttggg
     5581 cttgccttta tgattgacaa atagaaagtt gcctcgtccg tctatcacaa atggttctgc
     5641 ctttgggcgt ttctttataa cccgttgaaa tgcctgtatc gtttctctac ttaatggcac
     5701 ttgccttgtt ccgctttttg tcttaggcgt ttcaatataa tagccctgtt ccttgctctt
     5761 taataactga tggttgataa ccacaacttc atggataaaa tcaatgtcca tgattgttag
     5821 tccgcacagt tccgagatac gaagtcctgt ctttaacagt atcagcacat catcataata
     5881 cttgtgatac acattgtccg tcttgatgaa tgagagtagg gcttgttcct gttcctctgt
     5941 caatgcgact ttctctttgg tatcattttc tatgacttca cttaacttga aatcaaaagg
     6001 gtttttcctt acacaatcgt cttgtatggc gatatagaat gacgctttta acgagcgttt
     6061 atggttgtta atggtgttat aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
     6121 agcgtcagag ggttttatcg tatcaatgct cctagcacct aacttgtcct ctttcaataa
     6181 ccgcatgagc tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
     6241 ctgttt
//
LOCUS       DAKBNN010000212         6233 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000212 DAKBNN010000000
VERSION     DAKBNN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.218"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            167..1648
                     /locus_tag="OUB02_003351"
     CDS             167..1648
                     /locus_tag="OUB02_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HCT8887079.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1681..5118
                     /locus_tag="OUB02_003352"
     CDS             1681..5118
                     /locus_tag="OUB02_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCT8887080.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYYIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYQR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNGLENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5136..5882
                     /locus_tag="OUB02_003353"
     CDS             5136..5882
                     /locus_tag="OUB02_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCT8887081.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5831..6151)
                     /locus_tag="OUB02_003354"
     CDS             complement(5831..6151)
                     /locus_tag="OUB02_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCT8887082.1"
                     /translation="MKKIMNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
BASE COUNT         2595 a          615 c          939 g         2084 t
ORIGIN      
        1 atatatttgt acccttaact attgttatct tttaaatgat aatgatattt atttaaatac
       61 tctttattaa caatttataa aacttaattg taaaaaatac ttattaatag taaaataaag
      121 tgaatcaatt atattataat tgctaaagag agtgaggtgt agctatttga aaaaaaatag
      181 attaatagca aagaatatat caatagcatt tatagtacta ttctttttta gtgtatttac
      241 atttttttat gtaggtaata ttaatagagt gttagaatat gaaacaaatg acataataac
      301 agttacaatt gctggatgga ttatactttc atttttattt ttaggtatta taatttacat
      361 attatattca aaagcaaata gtcaaaaaac tatagaaaaa gtagcatata cggattttgt
      421 aacaggttat tccaactgga gaaagtttga attagatgtt acaaatttat taaagaaaac
      481 atcacaaaat aataaatatg caatggtcat attcgatatt gataaattta aagcaattaa
      541 tgatatatat ggccataaaa agggaaattt aatattaaaa gatattgcag atactttaaa
      601 tgaattgact gatatcaatg aaacttttgc tcgtgtaagt gctgataatt tcaatatatt
      661 attaacatat aacaaaaaag aggatataat aaatattatc aaaaagatta tggcaaataa
      721 tgaacttgta aacttatctt ttggaattta tgaaatcaaa gataaggatt tatctgtaag
      781 tgtatatagt gatagagctt cacttgcaaa atcatcaata aaaaataata gtgatgtaaa
      841 ttttgcattt tttaatgaca aattaagaga gaaattatta tttgaagata agatagaaaa
      901 agaaatggaa tatgctcttg aaagtgggca attcgtaatg tatttacaac caaaatacaa
      961 tattaaatta gataaatttt gtggaagtga agccctagtc agatggcaat atacagaaaa
     1021 agaggtcata tatccaggtg attttattcc tatatttgaa aaaaatggat ttattagaaa
     1081 gattgatatg tacatattag aacaagcttg taaagaaatc cggtctttat ttgataaagg
     1141 aatttcacct ttacctatat ctgtaaactt ctcaagagtt gattttttta aaaaagattt
     1201 tattgaaaac atagtaaata tatgtgatag atataaaata ccatatagtt taatagaaat
     1261 tgaaattact gaatctagta tgtttggaga tactgatact ctatttaatg ttagtagaaa
     1321 cctacaggat attggcttta tagttgcaat ggatgatttt ggctctggat attcttctgt
     1381 aaatatgctt aaaaatatac cattaaatgt aataaaatta gatagaggat tttttgttga
     1441 tgataaagat gttgataaga gtcaaattgt tataaaaagc atagtttctc tcattaagca
     1501 attaggaata agggttgttg ctgaaggaat tgaaacaaga agtcaaatag aaatgttaaa
     1561 aaaagcaaat tgtgatattg ttcaaggata ttatttttca aaaccattac caattaaaga
     1621 atttgaaaaa ttagtctata aaatatagtc agcgagtctt aaatgggagg tggtgattaa
     1681 gtgataagag caaaatcgtt aaaaaatgta ctgagtattg ttttaatatt tttatttaca
     1741 ataatttctt tttctgattt tgcatttaaa actaatgtcc aggaagaaga aaatagtaaa
     1801 attaatttaa gagaaatatc cctacaaagt tccaatgcta taaaaataaa attagatagt
     1861 tctctatcct ttattaaaag tgtggcaaat atggtttctt attttaatac tatagataac
     1921 tcaaaaatag ttccattatt agctaatata tcaaaagaat ctgactttca aagaatctct
     1981 attgtatctc ctgatggaat aagtcatacc agtgatggca aaattatcaa tgtgtcaaag
     2041 tatcagtatt ttaagaaagc tattaaagga gaaagtaata tatctgaagt tgtgaaatct
     2101 ccaattgatg gaaaagatac ttttgttata gctactcctg tatttaataa gaataacaaa
     2161 ataattggtg ttttatacgg ttcttactac aatgataaat tagctgaatt tatagatata
     2221 acaagtttta atagtgaagg gtacatagat atatttgaat ctagtggtca atttgtttta
     2281 aaatcttcac atacaaacaa actgattaag tcaagtagta acatatatga tatatttaaa
     2341 aaagcttctt ttgatgatgg ttatagctat aaaaaaatat atgaagatac aaaaaataac
     2401 aagagtggct ttacctcata tgaatacaaa ggagaaaatc gttatgctac ttatacaccc
     2461 attggtgtaa atgactggta tgtaatttct gtgataccac aatatactgt aagaagaaac
     2521 attgataaaa taaatcaatt agcattttct cttattttca aggtattctt attgtttatt
     2581 atgcttataa tatatattat ttataaaaac aataaatctc agaaagatat actaaaagct
     2641 aacaaagaaa tctcatctat tacaaatagc attcctggag gagtacaaaa gtgtaccctg
     2701 ggtgagacat gcaatattaa ttttttaagt gatggattta tagagttgtt tggttataca
     2761 agagaagaaa taaaaacatt gtttaataat gatttttata gtttaataca taaagatgat
     2821 gttgaaagaa taaaaaaaga aatttttaaa caactttaca aaaataaaac tgttgaatta
     2881 gaatatagga ttataacaaa aaatggtaat acagtatggg ttttagataa aggggagctt
     2941 ataacagaac ctgatggaaa agagtatttt tgttgtgtaa ctgtagatat tactagaaca
     3001 aaacaattag aacaagagat ggaacaaaaa aatctagaac ttgaaatgat atcagaaaat
     3061 gttttaggtg gtataattat aacagaattt aatgatgatt ttactataaa atacgctaac
     3121 tctggttatc tttcaatgat tggatataca cgtgaacaat tagaaaatga acttaaatac
     3181 aaagcttgta atcttgtttt ttatgaagat agaaagaagg ctcttgaaag tatacaacat
     3241 caattaaaca caaaaggaat agctgtatat caacatagaa taagaagaag agatggtaat
     3301 attatttggg tttctataaa aggaaggctt attgttgatg atgatggtat tgaaaaaggt
     3361 atttggattc taaatgatat aacagattta aaaaatgcag aacaaaaact taaaataaat
     3421 gaagaaagat ttagaattgc attaaagcaa acatcaaaca ctatatttga gtattatata
     3481 aaaacaaagt cacttttaaa tactagtaaa gagcttgata aatataatat ttataaaatt
     3541 actaaaaatg ttccatattc tctagtagaa tcaggagtta ttcataaaga ttttagtgat
     3601 gattttataa acatgtatga aaatgtaata aatggagcaa aaaaagcaag ttgtgtagtt
     3661 aagatgaaac ttccagatga aaattatata tggaataaag ttaccttgac tactattttt
     3721 gatgaagatg gtaatcctgt aagagcaatt ggtatacagg aagatataag caaacaaaag
     3781 gaagcagaaa taagatacaa tcaagaaggt aagtatcatt cagctatatt gtcggaagca
     3841 atgataacct atgaaataaa tgtatcaaag gatactttta tcaaaggaaa tgaaagttgg
     3901 caaaaaccat ttggaattaa gcctgacaat aactattctc aaatggtaaa aactatagca
     3961 aatagtattg tacataaaga caataaagaa acatttttaa atacattttt atgtgaaaac
     4021 ttattggatg catataaaaa taatgtgact gaaataaaat tagaatatca aagaatgaat
     4081 caagaaaata aaatgatttg ggtcatttgt acaatgcact taataaaaga ttctattacg
     4141 aacgatataa aagcacttgc ttatgttaaa gatattgaca agcaaaaaaa tgaagaaatt
     4201 agattgaaat taaaagctga aagagatact cttacaggat tatataataa aggtactaca
     4261 gaaaaattga ttaatgattt cttgaactct gaggaagcaa gcttaggaat gcatgcattt
     4321 ttaattgttg atatagataa ttttaaatct gtgaacgata atttaggaca tgtatttgga
     4381 gataaagtat tatttgaagt ttctaaaaac ctaaaaccta tatttagaaa agatgatata
     4441 gtaggtcgaa ttggtggaga tgaatttata gtattcttaa aaaatattca gtcttttgag
     4501 aataccaaaa agaaagcaga aaaaatatgc gatatattta gaaattctta tacggaaaat
     4561 aaaaagacat ataaaatatc tggaacagtt ggaatttcat ttgtaccaga acatggaact
     4621 gtatttgaag aactttataa aaaagcagac atagcacttt attatgcaaa gagcaaagga
     4681 aaagataagt atgcacttta taatgattgt atctaccaaa atggacttga aaataatata
     4741 agtgataata atataaaatt agcagataaa tgtgagaaat ccaaaccagt acttaaagaa
     4801 attcttgatt ctattgataa ttatatctat atagtgaatc caaatactta tgaacttctt
     4861 tttgtcaata ataaggtgtc tcgtttggat ttagagataa aattaggaga aacctgttat
     4921 aaatcactat taaaatctga tagaccatgt aataattgcc ctcttaacgg attagaagaa
     4981 aataagcatt caaaattcaa aatagagaca aatacatttg gtaaaaatat cagtatagca
     5041 agttggatta attgcataga tggagaatac aactgtctta tagagtgtat agattcttct
     5101 ttatacaaaa ataaataaaa aaggactatg aagatatgtt aaatagtata actttgatac
     5161 ttggtataat atttacaatt tactattttt atctcaaaat agtttttgga ggaatttcat
     5221 ttagtgagat tttcttagtt gtaggaattg tactaattat ctatcaaata tttaagaaaa
     5281 aaattaaaga aaagaaggtt ttttataggg tgttaaaaat aataatcagc atttttttaa
     5341 tagtatttgt agttactgag agtctaatta ttttttatcc taaaaatagt ttagaatcaa
     5401 aaagtgatta tctcttaatt ttaggagctt ctgtaaaaaa gactacacca agtacaaccc
     5461 tgaagggtag gttagacact gcattgaaat acctaaaaat taatgataat tgttatgtgg
     5521 tagtatctgg tggtaaagga agtggagaaa aaataactga ggcaaaagct atgaaagatt
     5581 atcttataaa aaatggtata gataaaaata ggataataga agaggataaa tccacgaata
     5641 cttatgagaa ttttaaatat tcaaaattga agatagaaga acatagtcaa agaaaactaa
     5701 gcgatttaaa agtaaaaata gtaacaacag attttcatgt tctcagaagt aaaatattag
     5761 catatagaaa tggatataaa aatacgtctt tttatgctag taaatcaaaa ctttcatttg
     5821 taccaactta ttatacaaga gaattttttg cattatggaa aacaatcatt tttgatagat
     5881 aataaataaa aagactagat ataaactcat atctagtctt ttctattccc ttaattctaa
     5941 tccattttcc aactttactt gttgtagaaa tttcaacatt gggatttaca agtaattgct
     6001 tataagaagt tttatatttt acgattccaa aatataattt tccttcataa tccattataa
     6061 atccgaatgg tcttactctt ggtttattat cttcaactgt agcaaaatag aatgttgagt
     6121 tttctttaaa aaaattcatt atttttttca tgattaaatc ctctttgtat cttatactaa
     6181 tataatgaat ataaataaaa tattcataat actaatataa atcgatataa aaa
//
LOCUS       DAKBNN010000213         6158 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000213 DAKBNN010000000
VERSION     DAKBNN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.219"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            77..946
                     /gene="galU"
                     /locus_tag="OUB02_003355"
     CDS             77..946
                     /gene="galU"
                     /locus_tag="OUB02_003355"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCT8887083.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            970..1983
                     /gene="galE"
                     /locus_tag="OUB02_003356"
     CDS             970..1983
                     /gene="galE"
                     /locus_tag="OUB02_003356"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCT8887084.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            2095..4029
                     /locus_tag="OUB02_003357"
     CDS             2095..4029
                     /locus_tag="OUB02_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HCT8887085.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            4184..5047
                     /locus_tag="OUB02_003358"
     CDS             4184..5047
                     /locus_tag="OUB02_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HCT8887086.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(5255..6136)
                     /locus_tag="OUB02_003359"
     CDS             complement(5255..6136)
                     /locus_tag="OUB02_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8887087.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIAFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
BASE COUNT         2438 a          701 c         1151 g         1868 t
ORIGIN      
        1 tttgaagaaa aagtataaat atggtatatt aatttggtac aatagaaaat ttacgaagaa
       61 gggtgaaaaa atgcagatga cagttaaaaa agcaataata ccagcagcag ggctaggaac
      121 tagattttta ccagcaacaa aatcacagcc caaagaaatg cttcctatag tagataaacc
      181 aactttacag tatataatag aagaagctat agaatcggga attgaagaaa ttcttataat
      241 aacaggtaga aataaaaaat ctatagaaga tcattttgat aaatcagttg aattagagtt
      301 agaactagag caaaaaggta agacagagat gttagaaatg gtaagagaca tatcaaatat
      361 ggtaaatatt cattatataa gacaaaaaga gccaaaaggt cttggacatg caatttactg
      421 tgcaaaaagt tttataggtg atgaaccttt tgccgtatta ttaggtgatg atatagtaga
      481 ttcagatgtt ccgtgtttaa aacaattaat agatacttat aatgagtata aaacaactgt
      541 attaggtgtt caaaagatag ctaaagaaga tactaataaa tatggtatat tggatgtaaa
      601 acatatcgaa gatagagtat ataaagttaa agatatggtt gaaaaacctg caattgaaga
      661 agctccatct gatatagcta tacttggaag atatataata actcctgcaa tatttagcat
      721 attagagaaa cagacacctg gtaaaggtgg agagatacaa ctgacagatg cacttcaaac
      781 tcttggaaaa caagaggcta tttatgctta taactttgaa ggtagaagat atgatgtggg
      841 agataagatt ggttttttag aggcaacaat agactttgct ctaaagagag aaaatttaaa
      901 agatgattta atggactata tgagaaaaaa agtaagtgaa aaataaatat tagaaaagag
      961 gtaattacaa tggcagtttt agtagctgga ggagcaggat atataggaag ccatacagca
     1021 atagaacttt tagaatcagg ttatgaagtt gttatagttg ataacttaag taatagcaac
     1081 tcaatagtgg ttgatagaat caaggaatta tctaaaaaac cagtaaaatt ttataatatt
     1141 gatatcagaa ataaagatga aatgcatgtt gtatttaaag aaaataatat tgaatcaatt
     1201 atacactttg cagctttaaa agcagttggt gaatcagtag aaaaaccaat tgaatattac
     1261 agcaataatc taataagtac cttaaattta tttgaattaa tgagagaata tggtgttaaa
     1321 aagtttgtat ttagttcttc agctactgta tatggagacc cacatacttg tcctatatta
     1381 gaggatttcc cactttctgt aaccaatcca tatggaagaa ctaaacttat gatagagcag
     1441 atgttagtag atatatcaaa agcagataag tcattggata tagctctttt aagatacttt
     1501 aatccagtcg gtgctcataa aagtggtaga ataggtgaag aaccaaatgg agttccgagc
     1561 aatctaatgc cttatataac aaaaatagca gtaggtaaat tgaaggaatt gagtgtttat
     1621 ggtaatgact atccaacaca tgatggaact ggagttagag actatataca tgtattagat
     1681 ttagctgctg gtcatgtaaa agcattacaa aaattagaag aaaatccagg acttgttgtt
     1741 tataatttag gaactggaaa ggggtatagt gttcttgatt tagtaaaagc attttctaaa
     1801 gcaagtggaa aagaaatacc atataagatt gtaggaagaa gggctggaga tgtagccatg
     1861 tgttatgcag attcttcaaa ggcagaaaaa gagcttggat ggaaagctaa gtatgaactt
     1921 gaagagatgt gtgaagattc atggagatgg caaagtatga atcctaatgg gtatgaagag
     1981 tagaatacat acttatatgt ataaatgtaa caaatttgta aatataaggt attggtaatt
     2041 ttatgttata attagacaca tgtagtaatg tttctaaaag agggggaagt tatagtgaaa
     2101 aaaagtctaa aaaagtattt agtactagca ctgactttag ttttagtatt tgcatgtggc
     2161 gtaccagaat caagcgcagc cagtaaacat gtgattatag taaattcaag aaaaaatact
     2221 ctaggatatt ttgtaaataa taaacttgta aaagaattta gagttgcaac aggtaaaaag
     2281 gggtcagaaa caccaacagg gaaaacaaaa gtagttaaca agataaaaaa tagaccatat
     2341 tataaaggta atatacctgg aggaagtcca agaaatccat taggagatag atggatggga
     2401 ttagcattaa aaggaactta tggagataca tatggaatac atggaaataa taatgagagt
     2461 tctataggaa agcatatttc tggtggatgt ataagaatgc acaataaaga tgttagatgg
     2521 ttatttgacc aagttcctgt aggttcagat gtaattatag attatagtaa cgacagctat
     2581 gtaaagatag cggcaaaata taagataaat ctaaatcaaa caggatggaa aacagaaaat
     2641 ggtaagaagt attatgtaaa atctgatgga acataccaaa agaatagttg gctaaaagta
     2701 aatggaaaaa tgtattactt tgatgcttca ggagtaatgc aaacaggatg gaaaacaatt
     2761 aataacaaaa aatattattt aggaactgat ggagcaagag tatctggatg gaaagtaata
     2821 gacggaaaaa cttattactt taactcagat ggagtaatgc aaacaggatg gcaagaaaag
     2881 aatggaaaga aatactatct aggatctgat ggtttagcag taacaggatg gcaagaaata
     2941 gatggtaata agtattactt tgacaaaaca ggaataatgc agacaggatg gcaacaaata
     3001 gatggtaagt cttactattt agacaaagat ggaaagatgc taactggttc tcaaaaaata
     3061 gatgggaaag attatacttt taatgaagat ggaactataa atcctacatg ggatacaatt
     3121 ataggtgcaa atagatttga tacagctaaa aagataagtt cagttggaaa ctggaatgct
     3181 gatagctcag atactgtaat attagtaaat ggtaatgcaa tagctgatgg tataacagca
     3241 actcctttag caagtagcta tgactcaaca atactactta ctaatacagc taatttacca
     3301 actgaaactg ttgaagaaat gaagttatta gcacctaaaa cagtaatctt gataggtgga
     3361 gaaaatgcaa taagttctaa attagagcaa gaaataaaaa ctactttcaa cacagaaaca
     3421 aagagaatag caggtcaaga tagatatcaa actgctacaa gaatagctga agaacttgga
     3481 aatcgagaag aaataaaaac tgcatatatg gtttctggaa atggggaggc agatgcttta
     3541 tctgtagctt ctaaagctgg ggaagaaaaa caacctataa tattagtcaa taaagatgga
     3601 attacagaag aatcttataa atggctaaca gaaagaaaat tagaaaatgc atactttata
     3661 ggtggaccaa gtgcaattaa cgattcagta atagcaaaaa tgaatgatat aactactgaa
     3721 gatatttcag gaaatagaat atatggagat agtagagtag atactaatgc caaagtaata
     3781 gaaaaattct atggagatac tgatttacag gctgtattag ttagtaaatc agatgcttta
     3841 gtagatgctt tatcagctgg accattagct gtaaaattac attcaccaat agttttaatg
     3901 gataattcag gattatcatc agagcaacaa agagtatttg caaataaaaa agttgaaact
     3961 ccttatcaaa taggaggcgg agtatcttat atagtaatgg ataaattaat ggatattctt
     4021 gcaaaatagt aaaaatatat attgaaaaat aattataatt gagatgtgaa attcaatcaa
     4081 taaaagcttg attgttttac atctcaattt tttagtataa aattaagttt aagttatata
     4141 aaaatcttgg aatattttta aaactcatat aaaatgaata tagatgctat catcatatgt
     4201 taagagggtg agtaagatta taaagaaaaa atatataatg tttattgatg aaagtggaag
     4261 agctgatata aatcatgaag acccatttac attgacagga gtaatttttg aatataaata
     4321 tgcagtaagt caaggcgatt atgtatgttc tttgaggaaa gaaatggatg catttaaaga
     4381 gtattgtttt ggaaccagta acataggaat acatttgaca gatatatcaa gaaaatcaag
     4441 agcatttgag aagtttgatg attcacagat taagttattc tatgatgagt tgcctttatt
     4501 tctatctaga ttagagtgta ctataatatc agtaacagta gataaagcta gattaaagaa
     4561 atattatgca ccttcaaagg aaccgtatgt tgtggctttt atacatgttc ttcagaattt
     4621 ttattcattt gtaaataatg aaactgtaga atctgcacga atagttatag agtcaaggga
     4681 tgatgtctca aacttaaaag tacaaaaggc gttttttgat gtatttaata atggaacaat
     4741 atacttagat atagatgaag aattaagaga taaggttaag ggttttataa ttgccaaaaa
     4801 agaagatgct gactatagag aaggtttaga aatagcagat attttatgta atcctcttag
     4861 cagagcaaga agagggttaa tagaagctaa tccaaagtgt atgcaatatg gagaaaaaaa
     4921 caaaatattc aaatctgtca gaaacaaaat atatactcca acgagtgtga atgatataag
     4981 aaattgggga tttaaagaag ttcctattgt agagcctgtt ggaccatggc ttgaaaaaat
     5041 gcgttagata caattgacaa attttaaaaa atctgatata atgtaagtaa cctagttatt
     5101 caggaactga atccagttgc tgttatataa cgataattac tgagactcgg atgccgactg
     5161 tcagttagga atctatactt ttaaaagtat agattttttt attttataaa aagaataaaa
     5221 gaggatattt aaaaataacc cctttattaa taaattataa ttgagcgttt ttaatgacaa
     5281 agtttttaaa gtttttaatt gcaggaggca aatatctatc ttttataaat gctaagtaaa
     5341 agtttctttc ccaacttggg tgaataatct gaattacttt tacttttgta aagctaagca
     5401 tattcatatt tgggactaca gcaattccga aattttttgc aactaatcca gcaattactt
     5461 ggtcttcctc tacttcatag actatattag gctgttgatt tattttttta aacatatcat
     5521 caattatata tctaagaccg cttctctgat taaatgctat ttgaggataa ggtatggttt
     5581 cttttaaatc tatagtatcc tttgcagcta gtggatgact atatggtaca attagtacta
     5641 aatcttgttt ggcaacaggt ataaattcaa ttcccttttc tttttcaagt ttcgaacaaa
     5701 atgcaatatc atattttctt tctttaagac cttgaataat gtcagtagtt acaccagtat
     5761 gaaatttaaa atctattgat ttagcaggat tagattttaa aaatttgtgc actatgtctg
     5821 ggataaaatc agtaccaagt gttctaagaa aagctaagtc tatttgtcct tcaccagtac
     5881 cagttatttt taatgatttt acacttgaat ctaaaatttc taatgatttt tccacatctt
     5941 ttaaaaatat ttttccatac ttagtaagga ctatatttct accttctttt tcaaataaag
     6001 ccaccccaag ttcattttct agtaaagaaa tagcatgact taagcttggt tgtgttatag
     6061 ataaattttc tgccgctttt gtatagtgtt ctaaatgtgc caatgtaaca aaatagcgta
     6121 agtgatataa attcaatttt tgttcatcct ttcatagg
//
LOCUS       DAKBNN010000214         6113 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000214 DAKBNN010000000
VERSION     DAKBNN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.220"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            287..853
                     /locus_tag="OUB02_003360"
     CDS             287..853
                     /locus_tag="OUB02_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HCT8887088.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELSKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            893..1474
                     /locus_tag="OUB02_003361"
     CDS             893..1474
                     /locus_tag="OUB02_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HCT8887089.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1531..2448)
                     /locus_tag="OUB02_003362"
     CDS             complement(1531..2448)
                     /locus_tag="OUB02_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8887090.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2578..4209
                     /locus_tag="OUB02_003363"
     CDS             2578..4209
                     /locus_tag="OUB02_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT8887091.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGTLFYPNLKVMEE"
     gene            4212..5159
                     /locus_tag="OUB02_003364"
     CDS             4212..5159
                     /locus_tag="OUB02_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCT8887092.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEFCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5444..5707)
                     /locus_tag="OUB02_003365"
     CDS             complement(5444..5707)
                     /locus_tag="OUB02_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HCT8887093.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5806..6051)
                     /locus_tag="OUB02_003366"
     CDS             complement(5806..6051)
                     /locus_tag="OUB02_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887094.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2211 a          831 c          994 g         2077 t
ORIGIN      
        1 gttctcattt agatagtatg cttagaaatt ttagaattat tgagtctaac tttattactt
       61 actttacttt ataacaatca aatacaaatt taaatattat aaaactacaa aataatttaa
      121 agcatataat gtcaatatgt ttaaattata aaatattcat gtagttatat aataaataat
      181 ttataaatat agccaactta gcctaaatac gtatataata tagctaatat ggttcttata
      241 cttgatacca tcaataaaac taaaaatata aaaaggagaa cttaaaatga gtgttagttt
      301 acaaaaagga caaagagtta gcttagtaaa agataataat cctgtaaaaa atctagtagt
      361 aggcttagga tgggatatga ataaattggg taaaaaaaat tatgacctag atgcatttgc
      421 aatagcattg actaaccaag ataaaatgaa tgttagaggt gacttggttt attttggaaa
      481 tcttaaacac ccttctaaag ctattataca cactggagat aacttaactg gaaaaggtga
      541 aggtgatgat gaacaaataa ttgttaattt agaagacata cctgaatatg tacataaaat
      601 agtttttgga gtttctatat acaaagctaa aaaaagagac caggattttg gtcaaatgaa
      661 caactctttt ataagattaa ttgattcaaa ttccaagcaa gaattatcta aatataatct
      721 tcaagaagac ttttctggta aagtaacagt tttggcagga gaaatttatc gtagaaatga
      781 agaatggaaa tttaatgctt taggaattgg tcaaaatgaa gagcttcgtg aattaattaa
      841 tacatataag taataattac aataaaaaat ttataatgag gagttggttt aaatgggtat
      901 aactttagca aaaggacaaa aagtaagttt aacaaaaagt aaccctggtc taaaaaaagt
      961 aatagtagga ctaggatggg atattaataa atatgatggt ggctttgatt tcgatttaga
     1021 tgcctctgca tttttaactg gaactgatgg aaaagtaacg aatgatggag actttatatt
     1081 ctataataat ttaaaacacg cttctggagc tgttgaatat atgggtgata atagaacagg
     1141 tgttggtgat ggtgatgatg aacaaataaa tgtagactta tctaaaatac ctcaaaatat
     1201 tgctaaaata tcatttagtg ttactataaa cgaagcaatt acaagaagac aaaatttcgg
     1261 acaagttgaa aattcatata taagaatata taatgaagaa actaatgagg aacttattaa
     1321 atatgaattg ggtgaagatt ttagcataga aacagcaata gttgtggcag aactatatag
     1381 acataatgga gagtggaaat ttaatgcttt aggttctgga tttgaggatg gacttgcagg
     1441 attgtgtaaa aattttggcg ttaatatagg ctaatatatc taaataaaaa taagagtata
     1501 tttatatact cttattttta tttagatata ttataaactg tttacacttc tactcttctt
     1561 tctcgcttgt tttttcccat attctactga aatatcaaaa aatgctgaaa tattttctgt
     1621 aaaaaacttg cttctatgat atgcaatatc aaatgtccta tgtaaattaa taccttcaat
     1681 atcacaagcc cacaaaagat gcttttttaa ataatcctca ataagtaatt cggatataac
     1741 tgcaatgcca aaattatcta caactgctct taatatagct tcagagttat aacatgacca
     1801 tctaatattc attgggattt ttaattcttt taattgcttt tctaattgtg ctcttgtacc
     1861 acttcctaat tcacgcataa ttaatggttg atttgaaagt tctgaaactt ttatagagtc
     1921 tcttttgtag aactcatgct tgtgtgaaca tataagtaca agattatcat taataatgct
     1981 ttttgtaaca atatctgggt gcttaatttt tccttctaca atcgcaatat ctaattcact
     2041 attaagtaat ttttttgcaa tcagtcttgt atcttctaca aatacttcag gttctataag
     2101 aggatttacc ttgtataatt ctaacattat tggacttatt acacatgtac caacagttac
     2161 tgttgcccca actcttatac ggatattttt tgcacaatgc ttcatacaat cattcatttc
     2221 ttcatttaca gctaaaagat gcctcccata tccaagaagt tttttccctg cctcagttaa
     2281 atataatttt tttgaaattc tatcaaatag cttaacacca tactcttttt ctatttctga
     2341 aatagcttgg ctaactgatg actgggtaat atacatattt cttgctactt cactcatttt
     2401 accacattca gcaaccttaa caaagatttc aagactacga attgtcatat tatcacaacc
     2461 ttagcaaaca ttagtaaatg cttatgattt tattaatatt ttagtatttt acaaatatat
     2521 tgtcaagaag tataatttta ttatagagtt ataaagtaga ttggagtgtg atatattgtg
     2581 aaagtattaa ttataggtgg agttgctgct ggaacaaaaa ctgctgcaaa aataaaacgt
     2641 gaaatgggtg ataattgctc tgttacaata ctaaataaag gtgatgatat ttcttatgca
     2701 ggttgtggtc ttccatatta cgttggtaag ataattgaag ataaatcatc tttaatcgta
     2761 aatacacctg aaagtttttc taaactaaca ggagcagaag ttcgttgtgg agttgaagtt
     2821 acatctattg atagaaatgc aaaacaagta actttgaaaa cgctttcaac aggagaaaaa
     2881 gaaaatctaa gctatgacaa acttgtaatt gctacagggg cagacccaat aaaaccacca
     2941 atacctggaa tagatttacc tggagtattc tttatgcgta ctccaaatga tgctataaaa
     3001 ctaagagatt ctattgaaaa cggtattaag agagctgttg ttattggtgg aggtttcatt
     3061 ggtcttgaaa ttgctgaaaa tctttctgca atgggtatac gtgtaagtgt tgttgatatg
     3121 gctgaacatg ttatgcctgg atttgatact gattttgctg aatacgttga aaatcatatg
     3181 gctgataaag gcataatgat ttttacaggt gaccaggttg taggtattga aggtgaaaat
     3241 aaagttgaaa aacttcgtac taaaaataga gctattaaaa cagatttagt ggttatgtca
     3301 gttggaatcc gtcctaacac aggttttcta aaagacacag gtttagaatt tgcccctaat
     3361 aatacaatta ttgcagatga atatatgctt acaaatgaca aagatatata cgtagttgga
     3421 gactgtgctt ttgtaaaaaa ttctttaaca aataaaccta catggtcacc tatggggtca
     3481 tctgcaaatc atgagggtag aatttgcgca caaaatatat ctggaaaatc taaaacttac
     3541 aatggtgttc ttggaacaac agtagtaaaa ttacctgagc tcaatgcagg aaaaacaggt
     3601 ctaagtaaag aaatagctga aaaagaaggt tataatgttt gctctgtaac catagctact
     3661 gatgataaag ctcattatta tccaggtgca tctaatttta ttataagaat ggttgctgaa
     3721 aaaaatacta gaaaattatt aggtgttcaa gttatgggac ctggagcggt tgataaaata
     3781 gtagatatcg ctgctactgc aataacttta ggcgctgacc tttattcatt agaaagtatg
     3841 gaccttgcat atgcaccacc tttttcaaca gctattcacc cttttgtagt ggcagtaaat
     3901 gttttacaaa ataaacttaa tggagaactt gatagtgttt tacttgacga aattgaggat
     3961 tttgacagtt ggactaaact tgatgtttca aaagctcctt ctattcctag tttacgttat
     4021 attcctgttg gtgaaattaa tggagaaatt gaaggccttg ctaaggatga aaaaattctt
     4081 cttatatgtg ctaaaggtaa aaatagctat atggcgcaaa atcgcttaag acgctttggc
     4141 tatacaaata cgaaagttct tgaaggtgga acactatttt atccaaatct taaggtaatg
     4201 gaggagtaaa tatgagtaat gttacagcag aacaaaaaaa ggaagtaaaa ggtagaggtt
     4261 ttctaccaag cagggatgga gaacattttg cagcacgtat aataactgta aatggtgtaa
     4321 taaatgcatc acaagcaaaa aaaattgcag aagctgctga aaaattcgga aacggtcagt
     4381 tagcatttac tactagacta acagtagaat tacctggtgt aaaatttgaa gatattgaag
     4441 ctctaagtga atttattgct agcgaaaatc taataacagg tggaactgga tcaagggttc
     4501 gtcctgttgt ggcttgtaaa ggtactgttt gtgttcatgg tctagctgat acacaggcac
     4561 ttgctgctga aatacacgaa gaattttaca aaaattggta tgatgtaaaa ctacctcata
     4621 agttcaaaat tggtgttggt ggatgcccta ataactgtat taaacctgac ctaaatgatc
     4681 ttggaattgt tggtcaacgt gtacctgact atgattcaga gctttgtgtt ggttgtaaaa
     4741 aatgtgctgt tgtagagttc tgtcctgtta aagcagctaa attaacagat aaaggtaaac
     4801 ttgaaataga tagtaatcta tgtaataact gtggtaagtg tatagaaagt tgtaattttg
     4861 atagtattga agaaaaagaa tctggttata aagtctatat aggagggaaa tggggaaaat
     4921 cagttcgtcc tggaacacag attgatagat tattttcaaa agaggaactt atgactttaa
     4981 ttgaaaaagc aattctttta tttagagaac agggtaacac aggtgaacgt tttggaataa
     5041 ctatcgatag aatcggtgct gataaattta tagaaatgct tttatcagat gaagttttgg
     5101 aaagaaaaga agagatacta gaggctcttt tacaccttac aggtggagct gtatgctaac
     5161 aataaatatt ttaattgtac agataaccta tggaaaaaat tttagttaag aataattaaa
     5221 attctatata tgatatatga taaatgaggc tatctcaaaa cacagatacg cctctttttc
     5281 attgttgaaa tgataaagta tatttgagtt catataaaaa agagtgtctc gtgaattata
     5341 aaattcattt tgaggcaccc acattatttt ataataattg cttattaatt ttattttaac
     5401 tatataaaaa gtacaaaaag taagaatatc caatattcta aaattaattt tgaattgtat
     5461 tgattgaaaa gtttactatt ccactatttc catcttgatt tactactaga taccatgttc
     5521 cagtattagg aactctcatt ctacatggat ttttctttgc aaatccacca tagtaatcat
     5581 aatttctttg atttttaaat ttcacataat ttatatggtc taacaatatt acattggctt
     5641 gtttctctaa atttacctct acaatatccc ctttttttaa gttcatctta ctataactat
     5701 attccattga attactcctc cagtatcgaa taatctttgt tatttactca tctcaaatat
     5761 gtaaaaattc atttaaaata ttacaaatac tatataatta gactattatc cagttttaac
     5821 cttatttact acaatccaaa ggttattaaa aatattattg gcaacaactt ctccatccca
     5881 tacaactctt tcttctttat tttctaataa agcatagtac ccagagtcag aactttcatc
     5941 tttatagact gtaacataaa actcttcatc actatattca ggtatatttt taaagtcttc
     6001 agatatttta catgttaata tataaccttc taattcttca tctataaaca aagcaattcc
     6061 tccaatctct atatttttac taattatacc atattatgtc ataaactttt tat
//
LOCUS       DAKBNN010000215         5990 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000215 DAKBNN010000000
VERSION     DAKBNN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.221"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..593
                     /locus_tag="OUB02_003367"
     CDS             <1..593
                     /locus_tag="OUB02_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCT8887095.1"
                     /translation="NLTDKISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKS
                     NKNINKEDDVKSKKNTVENEDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVAD
                     IGENKGNTSEATYQVAILSEKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYY
                     NNGDLKNSKLTLKKLVNKVPNSMYNNSKVKEILKEE"
     gene            709..2646
                     /gene="htpG"
                     /locus_tag="OUB02_003368"
     CDS             709..2646
                     /gene="htpG"
                     /locus_tag="OUB02_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCT8887096.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKHYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLFIEL"
     gene            3034..4164
                     /locus_tag="OUB02_003369"
     CDS             3034..4164
                     /locus_tag="OUB02_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCT8887097.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNSGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(4412..4651)
                     /locus_tag="OUB02_003370"
     CDS             complement(4412..4651)
                     /locus_tag="OUB02_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887098.1"
                     /translation="MKKEYGYPTLKVICQASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKIIMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(4656..5075)
                     /locus_tag="OUB02_003371"
     CDS             complement(4656..5075)
                     /locus_tag="OUB02_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT8887099.1"
                     /translation="MNPSSFEHIVRIQFNALMMTVIKCTVKNRNRQFARRSKREVLFC
                     ELSDTKNVECVTKDNYSCDYISFEVLNFTIQISNEKLAVALYKLSNKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTVLKERN"
     gene            complement(5267..>5990)
                     /locus_tag="OUB02_003372"
     CDS             complement(5267..>5990)
                     /locus_tag="OUB02_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8887100.1"
                     /translation="LSDFDTKEIMPFVKNAVPSVIQQSIPAISTTFLTAIVSTYSISA
                     IAGYGVAGKLEMILFYPAMALNMVLTSIVGQCIGAQRIDRAKNYLKLALKYGIVVSSV
                     LSAVVIFFSKQLAKLFVSNVAVTNVVSHYFLIVSIGYVLYTITSCYLGTLNGIGKPTK
                     SMILMIFYYIIIRIPFAYLLSWLAENLNGVWIAILISHIVACIITILTSNQIIKRLYG
                     KLYDRTKKARYKSIESNCCGKF"
BASE COUNT         2393 a          710 c          967 g         1920 t
ORIGIN      
        1 ataatttaac tgataaaata tctaaaaata atgaaaccaa aaatgaagag tcaatagatt
       61 ctaacttgac taagaaagaa aataatatag aagaaaatga agatatgagt aaggcttcta
      121 atataaataa gagtaataaa aacattaata aagaggatga tgtaaaatct aaaaaaaata
      181 ctgtagaaaa tgaagatgat aaagattctg acttatttac agaggatgaa ttgatgtcta
      241 aggcgaatac tttaaaatca gaaaagaaaa ctaaatcttc tatacaacta tataagaaag
      301 ttgcagatat aggtgaaaat aaagggaata catctgaagc aacttatcaa gttgctattc
      361 taagtgaaaa gttaaaagac tataaaactg ctgaagaata ttataaaatg tatgtagaaa
      421 attactcaga aaaagatgct tattttgatg aatcttatta taatttaggt atgatgtact
      481 ataacaatgg tgatttaaaa aattctaaat tgacattaaa gaagttggtt aataaggtac
      541 caaatagtat gtataataat tcaaaagtaa aagaaatttt aaaagaagaa tagttaaatt
      601 agataagaaa aataaattat tttattttaa aataatatga ttataatata ttgacatacc
      661 attttaaaaa atttaaaata ttaattataa aatgaggagg gataaaaaat ggaatttgaa
      721 aaagggagta tttcaattca tacagaaaat atttttccta taattaaaaa atggttatac
      781 tctgataaag atatatttat aagagagctt ataagtaatg gatgtgatgc agtaagtaaa
      841 cataaaagac ttgtatcact aggagaaata tcagaaaata agtcatcaga ttataaaatt
      901 acagtatctg taaataaagg agaaggtaca cttaaattta ttgacaatgg aattggtatg
      961 actgaagaag agataaaaaa atacataaat caagttgctt tttcaggtgc tgaagatttc
     1021 tttaataaat ataaggataa gatggaagag tcaaatgaca taataggtca ttttggatta
     1081 ggattttatt ctgcatttat ggtgtctaaa aaggtacaaa tagataccct ttcatataca
     1141 gaaggtgcaa ctcctgtaag atggatatct gaaggtggta cagaatacga aatatctgaa
     1201 tcagatacta gaaatgatag aggaactact ataacactat ttattgatga tgatagtaaa
     1261 gaatttttag atgaatttac tgtaagagga ataataaata aatactgttc tttcttacca
     1321 gtagaaatat atttagaaga tgttgaaaga ttagaaagag aagctaaaga agcagaagaa
     1381 aaagctaaaa aacaaaagga agatggaaaa gaagagattg tagatgcaaa agtaattgag
     1441 cctttaaacg acactaatcc tctatggtta aaatctccaa aagactgtac tgatgaggag
     1501 tataaagagt tttatagaaa ggtattcaat gtatttgatg aacctttatt ttggatacat
     1561 ttaaatgtag attatccttt caatttaaag ggtatacttt acttccctaa attaaaaaat
     1621 gagtttgaac tgactgaagg aaaagtaaaa ttatacaaca atcaagtgtt tgtagctgac
     1681 aatataaaag aagttatacc agaattttta cttttattaa aaggtgttat agactgccca
     1741 gacttaccat taaatgtatc aagaagtttc ttacaaaatg acagagacgt aagcaaaatt
     1801 tctaagcata taattaaaaa agtagctgat aagttaaaat ctctttgtaa aaatgaaaga
     1861 gaagaataca ataagttttg ggatgatata caaatattta ttaaatatgg ttgcttaaaa
     1921 gatgaaagtt tctatgagaa ggtaaaagaa tgtatattat tcaaaactat tgatgatgag
     1981 tatataacat tacaagatta tttagaaaaa tgtaaggaca aacatgaaaa taaggtattt
     2041 tatgtaagtg ataaggaaca gcaatctcaa tacattaaac tatttaaaga atacgactta
     2101 agtgctgttg ttttaaactc atcaatagat actcatttta tttcatttat ggaatataaa
     2161 gagaatggag ttaagtttaa tagaatagat gcagaccttt ctgatgtatt aaaagataaa
     2221 aatgaaaata aagatagtga agaaaacaaa gaagagatag ctaagataga aggtctgttt
     2281 aaagaagctg ttggagaaag agtgaagcat tattctgtag aaggattgaa aaatgaagac
     2341 actccagcta tggtgttagt atcagaacaa tctattagaa tggcagaaat gcagtcaaga
     2401 tttgctggaa tggatttagg aatgaatttt gaggaagaaa aaactcttgt aataaatgag
     2461 aacagtccaa taatcaaaaa attggtttct cttaaagatg atgaggaaaa gaaagataag
     2521 attacgctta tttgtaacca gatagcagat ttagctttac tttctaataa agaattaaag
     2581 ccagatgagt tagattcatt tgtacaaaga agtaataagc ttatgagtct tttcatagaa
     2641 ttataagatt gaatttgaat acattgaagc aaatttataa aaagtattca atatcagtga
     2701 tgataaagaa gtgtctcaaa atgaactttt cagttcatgg ggcgcttttt ttatgtgaaa
     2761 ttaaatttat tttaatcatt tcaacaatga aaaatatatt tatctctatt ttgagacaga
     2821 ccattcttta tttttttatc tttggttaat ttttaaatat ttaagtaggt tctaataaaa
     2881 aactctattt agttaaatat agttaacttg atatataata agataataca tataattaag
     2941 taaaagattg ttttttagtt tatgtttaaa tacataatta cactttgtta aaaacataaa
     3001 attattacta aagtagaaat aggaggtttt agcatgaatt ttaattataa tttgccagta
     3061 aatatcttat ttgggagagg cagaattaat gatgtgggaa aagaagtatc taaatatggc
     3121 aaaaaagtat tgatagtaac aggtaaaaat agtacaaaga aaactggatt acttgataaa
     3181 acaatagatt tactaaaaga ctctaaaata gaatatgaag tttttgatag agtagaacaa
     3241 aatcctctta taactactat atattcagga gtagaaatta taaaatcaac tggatgtgat
     3301 tgtgttttag ggcttggtgg tggaagtatt atggatgctg ccaagagtat agctttttct
     3361 tataaaaatt caggagattt aaatgattat atatttggaa ttagacaagg tgaagaagct
     3421 ttacctataa tcttagttcc aacaacatca ggtacaggta gtgaaggaaa ttgttttgca
     3481 gtacttacaa attcagaaac taaagataaa aaatctataa agaagaatag tatgtatgca
     3541 aaagcatcaa taattgaccc agaacttatg gttacaatgc caaaacatat aattgcatca
     3601 gttggatttg atgcacttgc ccataatatg gaagcatatc tttctaatgg aagaaaccct
     3661 cttgcagatg tacaagctat ttatgggatt gaacttatat cagaaaacct aataaaggta
     3721 tataatgatg ttaatgattt agaagcatgg gaaaagataa cacttgcaag tactattgga
     3781 ggaatgacga ttggtacagc gggtacagcc ttgccacatg gaatggagca tcctgtaagt
     3841 ggtcttagag atgtaatgca tggaaaagga cttgcagctt tgactcctac tattatagaa
     3901 agttcatggg atagtgatat tgaaagattt ggtaatattt caaaggtatt aggaggaact
     3961 tgtgcagaag attgttcaga tgcaattaga aatttcctta aaaaaataga tttagatata
     4021 aagctaagtg atttaggtat tgaaaaatca gatatagatt ggttaactga taattgtatg
     4081 aaaatatcag ttgcaaatat taagagacat ccaaagtatt ttaataagga acaaataaag
     4141 gaaatgtacc ataagtcctt ataatttgac tttacatatt tagttttata taaaaattat
     4201 aaattaataa taaaaaatta aagattaaat tttatatctg atttttgatt atccgcataa
     4261 acactatact ttcaaacatt ttgaagtata gaaaactatt cattttacca cgaatttacc
     4321 acgattgaat gagccttgat atgacacaaa aacaacatgg gagatagcac aaatatctgt
     4381 gtatctcccg tttttcattc atgtagtatg attattttac tctgatttca aatttcaaga
     4441 ttgctttcat catttctgta tggatttgac cttttaattc ttcatcaacc tgcataatga
     4501 ttttaccact ttcagagtgg taaaacggac gcaagcataa tttacatata taagcgtcat
     4561 aaaacttcaa aatctctctt atggcagttt catttccagc agacgcttga catattactt
     4621 ttaatgtagg gtagccatat tcttttttca ttggttcaat tcctttcttt caatactgtt
     4681 tttagtttct tcaatccgtt acttcttctg cgataaatag cagaacgtga tacatgatat
     4741 aattcggcta tttcttggtc gctcatactt tgaaaatagc gtaatagaat tacatcacgc
     4801 tctttattcg ataacttata taaagcaaca gcaagtttct catttgatat ttgaattgta
     4861 aaattcaaga cttcaaacga aatgtaatca caagaatagt tatccttagt tacacattca
     4921 acatttttcg tatctgataa ttcacagaat aaaacttcac gcttagaacg tcttgcaaac
     4981 tgtctgtttc tgttttttac tgtgcattta ataacagtca tcatcaaagc attaaactgt
     5041 attctaacga tatgctcgaa agaagatggg ttcataatct cacctccttt ccgcatacgc
     5101 tgaaatatat aaacggagat accgtctata tctttggaaa atacaaaaag aagaacaaga
     5161 aaaaagtccg tacaaaatat accgtctgta cgaactttta atattgtaaa atatttagtt
     5221 gttgataaaa tgctgttagg tagtgcataa tcgtataaga ttattcctaa aactttccac
     5281 agcagttact ttcaatgctt ttatatcttg cttttttagt cctatcataa agttttccat
     5341 ataaccgctt aataatttga ttacttgtta aaatagttat aatgcaggcg actatgtgac
     5401 tgattaaaat cgctatccaa acaccattta aattttctgc cagccaagat aatagatagg
     5461 caaaaggaat acgaataata atatagtaga atatcattag tatcatactt tttgttggtt
     5521 ttcctatacc atttaaagta cctaagtagc aactagtaat agtgtaaaga acataaccga
     5581 tactaacaat taaaaaataa tggctgacaa catttgttac agctacgttg gaaacaaaca
     5641 attttgccaa ctgtttagag aagaaaataa caactgctga taatacactt gaaacaacaa
     5701 taccatattt taacgccaat ttcagataat tctttgctct gtcgatacgt tgtgctccaa
     5761 tacattgccc tacgattgaa gtcaacacca tattaagagc catagcagga taaaataata
     5821 tcatttctaa ttttccagca actccgtagc cagcaatagc agaaatacta taagtactaa
     5881 caatagcagt taaaaatgtt gtactaatag caggaatact ttgttgaatg acagacggca
     5941 cagcattttt tacaaacggc ataatctctt ttgtatcaaa atcagatagt
//
LOCUS       DAKBNN010000216         5927 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000216 DAKBNN010000000
VERSION     DAKBNN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.222"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            240..1067
                     /locus_tag="OUB02_003373"
     CDS             240..1067
                     /locus_tag="OUB02_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HCT8887101.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2089..2553
                     /locus_tag="OUB02_003374"
     CDS             2089..2553
                     /locus_tag="OUB02_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887102.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFISLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRALKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2820..3464)
                     /locus_tag="OUB02_003375"
     CDS             complement(2820..3464)
                     /locus_tag="OUB02_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887103.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGGVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3394..4683)
                     /locus_tag="OUB02_003376"
     CDS             complement(3394..4683)
                     /locus_tag="OUB02_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887104.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIIINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4680..5552)
                     /locus_tag="OUB02_003377"
     CDS             complement(4680..5552)
                     /locus_tag="OUB02_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887105.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQSLIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEEKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2154 a          796 c          732 g         2245 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacgaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acaccttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatattttt aaagattctt tttgtaacta ctagaatact aatttagtag
     1681 ttattgtatc tctaaagttt aagcatggat agatatacat ttgataattt gcctataatt
     1741 tacctactac ttggagtatt ggagcaaata caatatatta ataataagtt cctaaagcca
     1801 ataaattaac taatacacat aaagatattg atgttgatta tacaactata agaaacggat
     1861 ttaacagaat ttaatcaagc taagaaatta tatatatcgt gatgtttttg aattagaagg
     1921 taattcaaaa ttagtttata gtcttctgtg aatccaataa aaagtacagg gtacaagaat
     1981 tgttagtaaa atttttaaag attttatgga taaatataaa tagttttact tattttctaa
     2041 aactttaaaa taccattaat taccttaaat attttaatag gaggacatat gaatttaaat
     2101 aaagttaaat ttactgaaga acatgctatt gaagttacta attggaaata tgaaggtaaa
     2161 tattcaatat acaatctacc tccatgggag gaaattaaaa agaagaattt ttcactagca
     2221 aaagaagata aaagaaaaaa ttttataagt tttataaatg acaataaaga attgataggt
     2281 tttataagct tattagatga aggaagctct gtattctttg gtattggtat gaaaccgaat
     2341 tattgcggaa tgggaattgg taaagaaata attggcctag gattacaaga atgtagaaat
     2401 aaatatagta ctaaaccaat tgtattaaat gtaagaacct ggaacatgag agctttaaaa
     2461 tgttatgagt ctcaaggatt taaaatagtt gagactaagg tacagaaaac gcatttagga
     2521 gatggagaat tttttgttat gaggtatcaa tagtttataa caatagtctt attgttaata
     2581 caacaattaa aataaaaaaa tacatatgtg taggtcatag caataagtgt ctttgaaata
     2641 taaatttgag agcttttgat aaaatttggt atacatatat attttatact acggatatat
     2701 aaatctatct acatacataa agagattgta agtaggtttg tgtatataaa aatagattta
     2761 tatttcataa atgtaccttt tatttgtatt acctttattt tatctaagct ctcattttat
     2821 tatctaatct tcctttttac tggttttatt aagagagatg caattatcaa cacaacactt
     2881 ccaagtatca gccaatattt ctcatcaaaa ctaccttgac tcatagacat tataaaaaat
     2941 tttccaacat acttgccttt agtcatcata tttaaaatta aatatcctat agatatcata
     3001 taacctatga ttgtattatt aaataaattt gagaaaaaca ttcctaaact tcctataaac
     3061 atagctgatg cgaaacttcc aataatatac tcaactactg gaaaattaca tccaagatat
     3121 attaaaacat ttacaaaacc tatgattaat accactatac ctataattga catcagtatc
     3181 ctaattaatt gtacaataac ttgagataat gcttttgaag atactatctc atcaatttct
     3241 tttgattgct ctagcgaaaa tacaggtacc aacattactc cccctattat tgcaacataa
     3301 ttttctaaac atatagctgt tgaaattctg tctaaattag ctactccttg tattagagga
     3361 atcatacata gcaatataat tgaacatatt aaattagtta aagtgcttaa tttgaagttt
     3421 tcttttatta gaactaaaca gttttgaaaa ctttgcccaa acatcaagat tcccccttct
     3481 tttttgctca tataagaata tagctaacaa aacaagcacc aatgctccca agcttatcac
     3541 aattctatta attataattt gattgaagtt ttgtataata atatctccat ttaatacact
     3601 attatgtctt atcattagcc ccattgttga ataatcacct ttgagtcctg tagaatttat
     3661 actgtaaaac caccaaatta attgcacaat aattgctact ggtgtatctg ttaatatagt
     3721 aatactcatt cctacagcgg taactatcat aagagttggt agtatccacc ataatgtata
     3781 ttttataaat gctaagttat caatagatat acctttatct ttagcaaaga aaatcaaagg
     3841 aagtaaatct ttcataccta aaattaatac aggcaatatc atcataaaga caagtgcaaa
     3901 atatcttgat aaaactattt tgcatgaaga tacttgtttt gaataaataa tctctctcat
     3961 tcttgaacgc ttatctttaa gtgacataaa aactgccaca aatactggaa atatacttaa
     4021 aactatacct atataatcgc aaaaaattct tgcataccca ttactaactt tatctttttg
     4081 tattgtatag ttgtaattct tcatagcttc atcataggta actggaacta ctccaaatct
     4141 ttgtaacttg tcaaaagcat atgcagaccc tcctcctaaa atgtcgtcta ctctttgcat
     4201 tatttcttca aatctttcaa gagatacttt agaataatct atttttatac tttcatcagt
     4261 tttccaaagt gaatttattt cgctaatact tttaccagtt atttcttcta tatatttttc
     4321 tatggtcttc gttttatttt ggtctaattt tatatactta actacccctg ttggatacgt
     4381 tgtatatgtg tttttttgat attctctaat taggtttata gttgcatttg gaatgacctt
     4441 ttctgttttc atagattctt tttgaccata tggatattga ttgtgtacag aataatattc
     4501 ctcaatattc tctacttttt gtggagcaac taatggattt ccatcattag gaatattttt
     4561 acttacatca tttgaaactg aacttccaag ttgagaaata tagaaaaaaa ctaaaactac
     4621 aataaaaata taatatatca tactctttaa tatattttta cattctttta taaatagatt
     4681 catatattat ctcctcccat tataagcata tagccatctt caatagttgg attacaattt
     4741 actgctgtct caaaaggttt atcatctgat ataatcctaa gtatagcctt tccaccatga
     4801 gtcaacattc ctataactat aaatctactt tttatatttt caatatcttt tttatctact
     4861 tcaatactat atactttctc ttcagctagt tttgctaatg cttctgttga gccattgtat
     4921 atgacttttc cactattcaa tatagctacg ttttcacatg tagcttctat atctccaaca
     4981 atatgtgtag ataaaataac gattctatca tttgcaattt cgcatagtaa atttctaaat
     5041 cttatacgtt cttctgggtc aagacctgct gtcggctcat ctactattaa aactctggga
     5101 ttatgaatta atgattgtgc tatcccaagc cttctattca tacctccaga taaagatttt
     5161 actttcacat tttgataatt agtaaggttt actttgctta ataactttgc tataattttt
     5221 cttctttttc ttagtggtat atccgataaa actgctaaat aatccattgc tttatatact
     5281 gtcatgttag gatacattga aaagtcttgt ggcaaataac caaccatatt tctaatttcc
     5341 ttagtattct caataggaac tccacacata tgtattgaac cttctgtttt tgataataaa
     5401 gtggctaaaa ttctcataaa tgtggttttt cctgctccat ttcttcctaa aagaccaaac
     5461 ataccactgt cgatttcaag atttatatta tcaagtgctt gttttttccc aaatcttttg
     5521 ctaagattgg atactgctat tttcatttcc atatataaat acacccccag aaacaatttt
     5581 atagaatttt atttttataa atttttactg tattttcata ataaattcta aaacttaaga
     5641 tattatttat aaatttctaa attttttcta aagattttaa tttttctgtt tttaattaaa
     5701 ttaaagtatc tttgtttctg aaatttttta agtttatgaa gttataattt aatatatatt
     5761 tattttattt gtgagtctat ttttatatat aatgaaaaat gtgataagta tccaaaagaa
     5821 ttactggata taataagata gggtatttag ccttttaaga taaataaaaa tatctgcaac
     5881 tatatattgc aaaaatttaa aactaattta tatatgctaa tataaat
//
LOCUS       DAKBNN010000217         5884 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000217 DAKBNN010000000
VERSION     DAKBNN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.223"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            27..1340
                     /gene="aroA"
                     /locus_tag="OUB02_003378"
     CDS             27..1340
                     /gene="aroA"
                     /locus_tag="OUB02_003378"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCT8887106.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            1318..2397
                     /gene="aroC"
                     /locus_tag="OUB02_003379"
     CDS             1318..2397
                     /gene="aroC"
                     /locus_tag="OUB02_003379"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCT8887107.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            2400..3596
                     /gene="pheA"
                     /locus_tag="OUB02_003380"
     CDS             2400..3596
                     /gene="pheA"
                     /locus_tag="OUB02_003380"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCT8887108.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            3643..4452
                     /gene="aroE"
                     /locus_tag="OUB02_003381"
     CDS             3643..4452
                     /gene="aroE"
                     /locus_tag="OUB02_003381"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCT8887109.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            4464..4997
                     /locus_tag="OUB02_003382"
     CDS             4464..4997
                     /locus_tag="OUB02_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCT8887110.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            5018..5857
                     /locus_tag="OUB02_003383"
     CDS             5018..5857
                     /locus_tag="OUB02_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCT8887111.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNKNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         2430 a          587 c         1060 g         1807 t
ORIGIN      
        1 tctaaatttt ttcaggaggt aagataatgg gaagtctaaa aatatatcca agtaaattat
       61 ctggagatgt aaaaatacca ccatcaaaaa gtatggcaca tagggcagtt atttgttcat
      121 ctctaagcaa tggaaaaagt agaatttcta atatagattt ttctgatgat ataatagcta
      181 ctataagagc aatgacatca ttaggagcta ttatagaaaa gaaagaagat attttagaaa
      241 tttcgggaat tttttctaaa gaaggtattt taaatagaga gaatcagttg aatcaaccta
      301 aattgacaat agattgtaat gaatcaggct ctacattgag atttttagta cctatatctt
      361 tagcatttga tggtgtaaaa aggtttatag gtagaggaaa tcttggaaaa agaccacttg
      421 atacttatta tgagatattt gacagacaga atataaaata tagttacaaa gaaaatcagt
      481 tagatttgat tatatcaggt aagcttaaac cagatgaatt tagagtaaaa ggaaatataa
      541 gttctcagtt tatcacaggt ttactgttta tattgcctac gctagagagt gattcaaaga
      601 taataattac tactgagcta gaatctaaag gatatttaga tttaactctt tcaacgataa
      661 aagattttgg ggtagaaatt ataaacaata actataaaga atttattata aaaggaaatc
      721 aaacgtataa agcgagagat tataaagtag aaggagatta ttctcaagga gcattttatt
      781 tatcagcaga tgctatagga gaagatatta gcatattaga ccttaaagaa gattctcttc
      841 aaggggatag tgaagtagtt gaaattttat ctaggatggg aatggaaata ttaagagaag
      901 gcaacaaaat aaaaggaata acaaatggtt taaatggtac tttgatagat gcatctcaat
      961 gcccagatat aatacctgta ttatctgtag tagcatcctt aagtataggg aggactacaa
     1021 taattaatgc tggtaggctt agaattaaag aatgtgatag acttcatgca ataaatgttg
     1081 agttaaataa acttggagca aatatagagg agaaagagga tagtttgata atcgaaggag
     1141 taagcaagtt aaatggtgga gtagaggtgt ggagtcacaa agaccataga attgctatga
     1201 cacttgcaat tgcttcttgt agatgtgata aaccaataat attaaaagat tttgagtgtg
     1261 tatcaaaatc ttatccacat ttttttaaag attttaagat gttaggaggt cgaattgatg
     1321 agtggaatat ggggaaataa tttaaaagta tcaatttttg gagaatctca tggaaatgct
     1381 ataggtataa atatagatgg tcttccttcg gggattgaac ttgaccttga taatattgat
     1441 aaagaaatga agagaagagc acctggaaaa aattctatat ctacttcaag aaatgaaagt
     1501 gatataccag aaattctaag tggatatttt aatggaagaa ctacaggaac gccattatgt
     1561 gcaatcataa gaaatagtga tactcgttca aaagattatg gagagcttaa aaacttaatg
     1621 agaccaggac atgcagattt cacaggtaat gtgagatatt ctggatttaa tgactataga
     1681 ggtggaggtc atttttcagg taggataaca gctccattag tattttgtgg ggctatatgt
     1741 aaacaaattt tatcacaaaa aggaatagag ataggagcac acataaaaaa aattaaaaat
     1801 atagaagata tgagttttga ttatgtaaat atttcaaaac aacagttatc aaatttgcaa
     1861 actttggaac tacctttact tgatttatca aaggaagaag ccatgaaaaa tacgattata
     1921 gatgcaaaaa atcaaggaga ttcagttggt ggtataattg agtgtgcagt agtaggtatt
     1981 aatgtagggc ttggaaatcc attttttgac tctgttgaat ctactttatc acatttattg
     2041 ttttctgttc ctgctgtaaa aggtgtagag tttggtttag gttttgaact tgcagatatg
     2101 tatggctctc aaagtaatga tgaaatgtat tatgaaggaa atcaagttaa gagcaaaaca
     2161 aataacaatg gaggaattat aggagggatt actactggaa tgccaataat tttcaaagtg
     2221 gcaattaagc caactccatc aattagcaga caacaaaaca cagttaatat aaaagataaa
     2281 aaggacgata ttttatatat taaaggcaga catgaccctt gtatagttca aagggctata
     2341 cctgtaatag aagcagtaac agccattgga atttttgatt tgatgaaagg acgataataa
     2401 tggaggattt aacgagatat agaaatgaga ttgacaatat agataaagag cttattcaat
     2461 tatttgaaaa gagaatgaat acagttcttg aaattgccag acataaaatg aaaaataaca
     2521 ctactatact tcaaaaagat agagaagaaa aagtactaag tagagccgta gataatttag
     2581 ataataaagc atattcacaa gaaacggtac agttttttaa ttctataatg gaaattagta
     2641 gaggattaca aaaaagattt atagataatg atggtgagca gaaaatcgag tttagtttag
     2701 attcatcaaa taagaatttt gatttgtcaa atgtaaataa atataagtct ttaaaagatg
     2761 aattaaataa aaaaaatatc ctagtaggct ttcctggaaa atcaggttca tttacagaag
     2821 aagcactaaa taaattcttc aacaaaaaaa tatcaaaaaa acaatttaaa gaatttgaag
     2881 atgttttcat agcacttaaa aataaagaaa taaactatgg aataatcccc atagaaaatt
     2941 catctacagg agctatctct gaaacttatg accttttgag aaagtacggt ttttatatag
     3001 ttggagaaga atgtataaaa atagaccaaa atttaatagg gataaaagac acaaagcttg
     3061 aagatataaa agaaatatat tctcatccac aaggtatagg acaatgtagt gaatttttaa
     3121 aacaaaatag tacatggaaa ttgataccat ttaataacac agcaacaagt gcagaacttg
     3181 taagaaaact tcaagataaa acaaaagcag caattgcaag caaaaaagct gcaagtattt
     3241 atggattaga aataataagt ccttgtataa atgatatagc aaacaactat acaagatttg
     3301 tagtaatcag taatcaaata cacattgaag aagaatcaaa caaaatgagt gtagtatttt
     3361 ctgttgaaca tgaagcagga aaactttata aagtacttgg atactttgct aaaaataata
     3421 taaatatgac aaaaatagaa tcaagaccta tgaaaaatgc ctcatggaga tatttctttt
     3481 atatagattt tgagtgttct atatacaatg ctaaagtgta cgatttatta gaattgatag
     3541 aacataatac agcatatttt aaatttatgg gagtatataa aaataaaact gaatagataa
     3601 gataaatttt tatttaattt ataattatta ggaggtgtaa gtatgaattt tttcggtcta
     3661 gtaggtgaaa aactctctca cagtgtttca cctcaaatcc acaagagagt attcgagata
     3721 ttgaatattg aaagtgctta taaaaacttt gaaatttcaa aagaagatat atctaagcta
     3781 gacggtgcta taaaattatt aggaatacaa ggggtaaatg ttactgtacc atataaggag
     3841 cgtattatga aatatcttga ttttatatca ccagaggcaa aacgtatagg agcagttaat
     3901 acaattttac tcagagaaaa tatgttatat ggttacaata cagattactt tggtcttgat
     3961 agtatgttta aaatggctaa tattgatgtt caaggaaaag tagcagttat attaggaact
     4021 ggaggtgctt ctaaagctgc tcttacatat tttattgatt ctggtataga aaagctttat
     4081 gtttcaacta gaaaaaagga tgataaaaaa ttattaaata gtaaagctat attaatagat
     4141 tatgaagaac ttaaacatat aaaaggagat ataatattaa atgctacacc agtaggtatg
     4201 taccctaatg taggaatttc accagtgtca aaatctatta tacaaaattt tgatatatta
     4261 atagatttaa tttacaatcc tggagaaaca gagtttttaa gaattggtaa tagtatgggt
     4321 aaaaaaactt gtgatggatt atatatgctg gtggggcaag caataaaatc tcaggaaatt
     4381 tggcaagata ctaaaataga taatagtatt ttagatgtta tatataatga actaaaatta
     4441 gaatttttgt aagggagatt gttatgataa ataaaacaaa agaaaaattg atattaatag
     4501 gtatgccagg tagtggaaaa actacaattg gtaaactttt ggccaaagaa tataattgta
     4561 gtttctgtga tatggatgat tatataattc aaatatctca aaaaagtata gcagagttgt
     4621 tttcagaagg agaagatatc tttaggaact atgagactca agcatgtaga gaactttcta
     4681 taagtgataa aacagtgata tctacaggtg gaggtgtaat aaaaaaggat gttaatatgg
     4741 aaatactaaa agaaacaggt ataataattt ttattgatag acctatacaa aagattttgg
     4801 aagatataaa tattaattca agaccattat taaaaaacgg aaaagataga ttgtataatc
     4861 tttataatga gagaataaat ctttataaaa aattttcaga tatagagatt ttaaatgata
     4921 aatcattaaa taatgctgta tataatatta caaatgcagt cagtgaaaat tttaaatttg
     4981 actttaaaga aaaataatct agggggaagt aaaaataatg aatattgtta ttgtaggact
     5041 aggagttata ggaggctctt ttgctaaagc attaaaaaaa gctggatatg aaaatgtatt
     5101 tggagtagat gtagatttag aaactttaaa aaaagcagaa aaagcaaaaa tcataaaaaa
     5161 aggatgtact acaggtaaag agttatttaa aaaagctgat ttaattatac tatctatata
     5221 tcctagatta gttgtagatt ttctaaataa gaataaaaac ttttttaaaa aagggactat
     5281 aattactgat actacaggaa taaaagaaac acttatcaat gatgtgttgc aaattattcc
     5341 agatgatata gattttatat ttggacatcc aatggcaggt agggaaaaaa agggtattga
     5401 ttttgcaagt gaacaagttt ttaatggtgc aaattatata attactccaa ctggcagaaa
     5461 caacataaaa aatttagagt tagttgaaaa tttaatttta gagattggat ttaaaagagt
     5521 aaaaaagtta acttcacaaa aacatgatga gattatagct tttacttctc agttacctca
     5581 tgttatggca gttgctctta taaatagtga tgaagaaggt agagatacgg gtaaatttat
     5641 tggagatagt tatagagatt taactagaat tgcaaatatg aatgaagact tatggagtga
     5701 acttttttta ggaaatagag ataatttact aaaagttata gaaaactttg aatcagaagt
     5761 aaatttagta aaagaagcta tatttaacaa tgataaaaat aaattaatag agtattttaa
     5821 aaaatcatca ataagaagag aagctttaga aaaataaata ttaatattta agctatatat
     5881 agat
//
LOCUS       DAKBNN010000218         5851 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000218 DAKBNN010000000
VERSION     DAKBNN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.224"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1253
                     /locus_tag="OUB02_003384"
     CDS             <1..1253
                     /locus_tag="OUB02_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HCT8887112.1"
                     /translation="IDKFVIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNE
                     ILRNLTKIIKSNIFEEEMFARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQE
                     EKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEK
                     EMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFER
                     NGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYN
                     IPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDI
                     LKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQ
                     GYLFGKPMPIEEFEHLIVSNLIRG"
     gene            1429..1797
                     /locus_tag="OUB02_003385"
     CDS             1429..1797
                     /locus_tag="OUB02_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8887113.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1799..2524
                     /locus_tag="OUB02_003386"
     CDS             1799..2524
                     /locus_tag="OUB02_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887114.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2518..3282
                     /locus_tag="OUB02_003387"
     CDS             2518..3282
                     /locus_tag="OUB02_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887115.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3504..4754
                     /locus_tag="OUB02_003388"
     CDS             3504..4754
                     /locus_tag="OUB02_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8887116.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDTKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            4945..5829
                     /locus_tag="OUB02_003389"
     CDS             4945..5829
                     /locus_tag="OUB02_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HCT8887117.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVKFDEEG
                     ITIPYPKMDLTVKELNKI"
BASE COUNT         2229 a          542 c         1027 g         2053 t
ORIGIN      
        1 ctatagataa atttgtaatt gatgctaaca aaatactatg taaaaatact caagtaaagt
       61 atgctttatt atatatagat attgataaat ttaaatatat aaatgattta tttggatatg
      121 aagttgggaa tgaaattttg cgtaatttaa caaagattat taagagtaat atatttgaag
      181 aggaaatgtt tgcaagaata tctgcggata attttattat aattatgaaa tatatagaag
      241 aagaagatat aacaaagagg ttaaaaacta tatttgaaga attagacttg tttaataata
      301 atcaagaaga aaaatataag ttagttttaa gttgtggaat ctattttata cttccagaag
      361 atagagatat caactcaatc atagataggg ctaatatacc tcataaaatg gcaaagggag
      421 gacataaaag ttcatatgca ttttatgata ataagattca tgaccaagag ataaaagaaa
      481 aagagatgga aaatacaatg ttttcaagtc tagaaaataa agaatttatt atatacttac
      541 aacctaagat agaacttaat acaggagaga tacaaggttc agaagcactt gttaggtgga
      601 aacgtcctga taaagggcta atacctccaa atgaatttat cccatttttt gaaagaaacg
      661 gttttgttat aaatttagat ttatatgtgc ttgaagaagt atgtatatac cttagaaaat
      721 ggattgatgc aggaataaat ccagttactg tttcagttaa tgtttcaagg atacatttat
      781 attgcaataa ttttatagaa acatacaaaa atataattga taaatacaat atacctgcaa
      841 agtatataga acttgaatta acggagagta tcatatttga taattttgat attttaattg
      901 atataatgaa taatttaaaa aaaataggat ttttaatatc tatggatgat tttggttcag
      961 ggtactcatc attaaatatg ttaaaagaaa ttcctatgga tatcttaaaa cttgaccaaa
     1021 aatttattat ggaaacttat aatagtaaaa gaagcaaaat aattgttact aaagtaatag
     1081 aaatggcaaa agagttgggt atgaaagtca tatcagaagg agttgagaca gaagaacagt
     1141 ttaaactttt aaaagaagta aagtgtgata tggcacaagg atatttgttt ggaaagccaa
     1201 tgccaattga agaatttgaa catttaattg tatccaactt gataagaggg taattaatct
     1261 gtaatataaa gaatttaata aattgtgtta aaaatggact ctaatgtagt gttcattttt
     1321 tttgtaaaat tttttatact tttactaaaa agtaattgac atatactttg tattattata
     1381 ttattgtatt aacaacttaa tacaataata taataaggag gcatgaacat ggaatgggaa
     1441 cttgataata acaaacctat atatatacaa ttagtagaac atttaaagtt aaaaataatt
     1501 tcaggggaaa ttaaaatagg atctaaatta gaaacagtga gagcactagc agaagatgcg
     1561 gaagttaatc caaatacaat gcaaaaggct ctaacagaac ttgaaagaca aggtttagtt
     1621 tatagtcaga gaactaaagg aagatttgta acagatgata aggaaaaaat aaaagctatg
     1681 aaagaggaaa tagctaatgt ggagataaat acattaaaag taaccttaga aaagcttgga
     1741 tatgatagag atgaaatgct aaaacttatt acagaaaatc tgaaaggaga attataaaat
     1801 ggataaaaat ataagcaatg tttctcaaga tggaatagtt gaatttaaag gtgtaaacaa
     1861 gagttatgga actaagaatg ttttaaaaaa tattgattta aatataccaa agggaaaaat
     1921 agtcgggctg ttaggaccta atggaagtgg caaaagtaca atgattaagc taatgaatgg
     1981 attattacag cctgataatg gtgaaattat gataaatgga atgaaaccat ccatagatac
     2041 taaaaaaata gtttcatatt taccagaaag aacttattta aatgattgga tgaaggtatc
     2101 agatttactt aagtttttct atgattttta tagtgatttt gatgttagaa gagcaaatga
     2161 gatgattaaa agcttagata ttgatgtaaa tgaaaagcta aagacaatgt ctaaaggaac
     2221 taaagaaaaa gttcaactta ttttagtaat gtcaagaaat gcgagcatat atatattgga
     2281 tgaacctata ggtggtgttg acccagctgc aaggtcatat atacttaaga ctatattaaa
     2341 aaattattct gaagatagta ctttactgat tgctacccac ttaataagtg aaatagaaaa
     2401 catatgtgat gagataatat tcatatcaaa aggagaaata gtgttacaag gagacgttga
     2461 agcaataaga gaagaaaaag gaaaatctat agatgcatta tttagggagg aatttaaatg
     2521 ttaggtaaat tattaaaata tgagttaaaa gcaagtggaa gaatatttat accgttatat
     2581 atagccatat tgatagtagc tgtatttaat ggaatattta tgaatacaaa tatacttcag
     2641 gtacagggaa taggaatact agtattaaca agtcttttta tggcattagg agttttaact
     2701 attgtagtta caattcaaag gtttagaaaa aatcttttgg gggatgaagg ttatttaatg
     2761 tttactcttc ctgttagcac tagttcactt atattatcta aatgtataac agcacttata
     2821 tacgctgtac ttagttttat agttgcagta ttcacttttg gagttcttat gctttttggt
     2881 acttctggaa ttttactacc tgaaatttta gatttgttta acactagttt taaatggatt
     2941 tctgaaaact ttttagatat tttattatta gtagtagtaa tgtttatttc atattcatca
     3001 tttattttgt tattatatac atcaatctca atgggacaat tgccaaaatt caataaacat
     3061 agaaacatag tggcatttgc ttcttttata gctataaata tagtaatatc aattgttgga
     3121 gatgcagttg gaagcatctt accaaatgaa gatacaaata tggtatatca tctttatcag
     3181 agtcctagtt ttatgcttgc aatacttggt agtcttgtag ttgcaatagc gttatttttc
     3241 gctacaaaat ttatattaga taaaaaatta aatttagaat aaatgttttt atatggagct
     3301 atcttttgag taatgttaaa agatggctct tattatttat gtagttaatt catgtatata
     3361 atatgaatag tgttttattt ctagttaaat atatacatat gttgtataat gatattgcgt
     3421 gtaaaaattt gtaagaataa ctatacactt tatataagtt atatcaagaa aatttaaaaa
     3481 tatatggatt tgagggagaa gatatgaaaa gttatacaaa ggctaagtgg agtgtttggg
     3541 gtataattac attttcattt gttttagtat tatttttaag gatgtcaact gctgttgttt
     3601 cagacaattt agcaaatgag ttaggtttta attctataca aatttcaaat atagcatcct
     3661 tttgcctata tgcatatgca tttatgcaaa tacctgcagg aattttgatt gataaatatg
     3721 gagcaagaaa aattagcagt cttggaataa taatggcaag tttagggtca attttatttg
     3781 gtttgattca aagtatagaa ttagcatata tttctcgtgt aattgtaggg gcaggaacat
     3841 cagtaatatt actttgtatt ttaaaaattc aagggagatg gtttaataag tcagaatttg
     3901 cttctgcaac agctaaattt tcatttgtag gaaatttagg tggagttctt gctacttttc
     3961 cacttgtgtt tttgtctgaa cttgttggtt ggagaaattc atttttatta atcggtataa
     4021 ttggtgttgt aattggatgt tttatgtata ttattgttag agatacacca aaagagtatg
     4081 gattcaatgt agatactgaa ccctatgaaa agtcagagaa ggttaatatt gttgatggta
     4141 ttaagtctgt tataaagaac aaatcaacat ggtataattc aatgataatg ttctcttttg
     4201 ttggtcttac aagtgcattt ataagcttgt ggggagtaag atacataatg gacgtttatg
     4261 gtgtgagtaa gagtttttct gcgtttatag tatcattttt tacatatgga tttatatttg
     4321 gttcaataat tatggatttt gtatttgcta aaataaggtc tagcaaattt aatataataa
     4381 aatttggagc aatgatagat ttgtttatat ggattgtaat tgtagttgta taccaggtaa
     4441 aaccaccaat tatagtttta ccaatatcat tctttattat gggatgtata gttatgtcac
     4501 atctacaggt ttttaatgat accaagtata aaaataaaga aatttattct ggactggcaa
     4561 ctagtgttat taatactttt gaatttatag gaagtggaat tattaatttg attatagcta
     4621 tctctttaca agtaaattca tataatatag ttgatggata taaaaaaggt tttgtagtat
     4681 ttatagtact aagtattata actatagtat catctcatat aggggtaaaa aatgatgatt
     4741 ttaaggctat atagtgtttt attaatagtt taatttagta ttatattttg catatatata
     4801 acaaatagtt tgcattttcc taatatgtat aattggaata taaatcttgt ttactatgta
     4861 caaatggtat tgtattatat taaatagaaa ataagaactt taaactataa tattggttct
     4921 aaaatataag gtaaggagtt ttttatggat acgacgaaaa tagatgatat aagcaagtta
     4981 gatttaaata atttagatat taataagatg gatatgggag gtataatgga aagtctgcta
     5041 gaatgggcta ctacaagcgg agtaaaactt ataataggca tacttatact ttctattgga
     5101 tttaaaataa taaaaaaatt tgtaaaccat gttatgttat tacttagtaa aagagatatt
     5161 gatttaactt taagaaagtt tttaaagtct cttcttttga gtgtacttaa agttgccgtt
     5221 ataatcattg ttctggaata ttggggaatg agtttgtcca gttttgctgc tgtgatagca
     5281 tcagcagggg tagcaatagg tttggcttta caaggtagtc tatcaaattt tgctggagga
     5341 tttattatac tattgattag accttttaaa gtaggagatt acgtagaagc agcaggtcat
     5401 ggaggaacgg ttgaacaaat aggtcttttt tatacacaat tggttactcc agataataaa
     5461 cagatactta tacctaatgg ttctgtatca aatgatagtc taataaacta ttcagcaaag
     5521 aatactagac gagttgattt gacttttagt gtagggtatg aagatgatat actacatgtt
     5581 agaagagtac ttaaagatat tgttaataat cataaattaa taataaatga accagaacca
     5641 tttataggag tagtagaaca tggagataat gctattaaat ttgcaactag agtttggtgc
     5701 aagacagaag attattggac aatttatctt gatttactag aagaggttaa agttaagttt
     5761 gacgaagaag gaattacgat tccatatcct aaaatggatt taacggtaaa agaattaaac
     5821 aaaatttaat tttttaatat agtgtttaaa t
//
LOCUS       DAKBNN010000219         5785 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000219 DAKBNN010000000
VERSION     DAKBNN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.225"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(281..2113)
                     /locus_tag="OUB02_003390"
     CDS             complement(281..2113)
                     /locus_tag="OUB02_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887118.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2106..4352)
                     /locus_tag="OUB02_003391"
     CDS             complement(2106..4352)
                     /locus_tag="OUB02_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887119.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4420..4881)
                     /locus_tag="OUB02_003392"
     CDS             complement(4420..4881)
                     /locus_tag="OUB02_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8887120.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5186..5752)
                     /locus_tag="OUB02_003393"
     CDS             complement(5186..5752)
                     /locus_tag="OUB02_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HCT8887121.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1847 a         1073 c          629 g         2236 t
ORIGIN      
        1 atttataata aaatttaaag aataaaatta aatatacatt ttgataatca cctccaacat
       61 tttccttact acatattata ataaaataaa cactttgtaa agacataaaa ttatgtggct
      121 atctcaaaat atagagaatc ctctttttca ttgttaaaat gataaaaata cattttattt
      181 catacacaaa agagtgcctt atgaattaaa aagttcattt taaggcaccc tcatttattt
      241 tatcttatta attacatgtc tatgctaaga tagttttttg ttacattgct tcatcttctt
      301 caaattgaga attgtaaaga ttggcataga atccgccttt ctttagaagt tcctcatgat
      361 ttccttgttc aactatatct ccttcattca ttacaagaat catatcagca tcacgtatag
      421 ttgataatct gtgggctata acaaaacttg ttctaccttc cataagatta tccatagctt
      481 tttgaatcaa tacttctgtt ctagtatcga cagaacttgt agcttcatct aatattaaaa
      541 ttttaggatc agctaaaata gcacgagcta tagtcaataa ttgtttttga ccttgagaaa
      601 cattacttgc ttcttcattt aatttcatct tatatccatc tggtaatgtc ttaataaatc
      661 tatgaacatg tgcagattta gcagcctcta taacttcttc atctgtagca ttcaacttac
      721 catatcttat attttccata attgaaccac taaataacca tgtatcttga agaaccatac
      781 caaacatttc tctaagctca ctacgattaa aatctttaat attatgacca tcaatcagta
      841 ttgaaccgct attaacatca taaaatctca ttaacagttt aactattgta gttttacctg
      901 ctcctgtagg tccaacaata gcaacttttt gaccaggttt aacatttact gagaaatcat
      961 ttattatagt cttgtttggg ttatatccaa agttgacatt ttcaaaatca attttacctt
     1021 ctaaaccatc tattgaaact gcattttcaa caatttggac ttcttcttcc tcttctaaaa
     1081 actcaaatac tctttcagaa gctgctgctg ttgattgcaa taaattggca acctgtgcaa
     1141 tctgagtcat tggctgtgta aagtttctta catattgtat aaatgactgt atgtcaccaa
     1201 cctcaattgt tttcttaatt gcaagccaac ctcctaagat tgataccatt acatatccca
     1261 agtttccaat aaacatcata agtggttgca ttatacctga taaaaattga gatttccacg
     1321 ctgaattata caggctatca tttaatttat caaactcctc aattacttca tcttcacgat
     1381 taaatgcttt tactattgtt tggccactgt aagtttcttc aacttgacca tttacatttc
     1441 ctaaatattc ttgttgatat ctaaagtatt tttgtgattt ctttattatt cttgaaatta
     1501 caaacattga tattggtaat attaaaactg ccactaatgt cataattcca cttatagata
     1561 acatcattat caaaacacct atcatagttg ttactgatgt tataagctgt gtcatacttt
     1621 gatttaaact ttgatttaaa gtatctatat cattcgtaat tctcgataat atttcaccat
     1681 gtgtcttagt atcaaaatac ttcattggca atctatccaa ttttgctgaa atctcttttc
     1741 ttaaattata ggatactttt tgagaaattc cactcattat aaatccttgt ataaaagaga
     1801 acagtgcact tatcaaataa aggaaaagta aaaaggttaa gatttttcca attgcattaa
     1861 aatctatacc tacgtttcct ccagaaactt tactcactag cccttcaaat atctttgtag
     1921 ttgcctttcc taaaattttt ggacctacta tagaaaacga cgcactacca atagcaaata
     1981 caattactag aattattgat attttaaatt tagataaata tgcaattagt tttcgcatag
     2041 tccctttaaa gtcatttgct ttttgtcctg ctcccataga ttttcccata ggacctcttc
     2101 ccattctact cattagctag ctcctccttt gaaagttgtg ataaagctat ttgctcataa
     2161 acttcgcaat tttttagaag ctctttatgt gttcctattc caactatttc tccctcattt
     2221 aaaacaatta tctgttctgc atttaagata gtactaattc tttgtgcaac tattagaact
     2281 gtactttcag cagtttttga ttttaaagcc ttacgaagag ctgcatcagt ttttaagtca
     2341 agtgctgaaa aactatcatc aaatatataa acttctggat tttttgctat tgctcttgca
     2401 atagaaagtc tttgtttttg acctccagat acatttgttc caccttgaga aatttcagtt
     2461 ttaaaagctt ccggtttaga atctataaat tctgtagctt gagctatttc agctgctgtt
     2521 ctcatttctt catctgttgc gtcttttcta ccatatttta agttggattc tatagtacca
     2581 gaaaacaata ctcccttttg aggtacatat ccaatttttt cccttaaatc atgttgagaa
     2641 acatttttta tattaactcc accaagtaat atttctcctt cagtaacatc aaagaaacga
     2701 ggaattaaat ttataagagt tgacttacca ctaccagtac ttcctataaa ggcagttgtt
     2761 tctcctggct ttgctgtaaa agatatattt gttaatatag gttcttctgc gtctggatac
     2821 atgaacgaaa catttttaaa ttcaacaaga ccctttttat cttccttaaa ctcttgtgcc
     2881 tctgattttt ctgggtcttt tatgcttata tctgacacta atacttcatc tatacgtgta
     2941 gctgaaacgg atgctcttgg aagcataata gataccattg atatcattaa gaatgacatt
     3001 attatttgca ttgtatattg tataaatgcc atcatatctc caacttgcat tgctccagaa
     3061 tcaacactgt atgcacctct ccaaactata agaactgtga taaggttcat tataagcatc
     3121 atagcaggca tcatacatgt cataactcta tttacaaaaa gatttgtttt agttaaattt
     3181 ttatttgctt tatcaaatct ttcttcttca tgtttttcag tactaaaagc tctgataact
     3241 ggaataccag taattatttc acggcttact aagtttaact tatcaacaag cttttgaacc
     3301 agtttaaact taggcataac cactgagaat aacactacaa ttaatgataa gatagcaact
     3361 actgctactg ctataatcca ggccattgaa gtatttgtgt ttaaaacctt tatcactcca
     3421 cctatagcca ttataggtgc atagaatact attctaagca acataaccat caacatttgt
     3481 atctgttgaa tatcatttgt actacgagta attaatgaag ctgtagaaaa ttcattcatt
     3541 tcagtatttg aaaatgacat aacttttcta aatactccac ttcttaaatt tctacctaca
     3601 cttgcggcta ctcttgctgc taaaaatcct actgttattg tagctaccat actaatcatt
     3661 gctataccaa gcattttagc accagtaaat aatatataat tattttgagt tttttctgta
     3721 tcaataccta ttttctcata ttctgacctt acatatgaaa cagagctttg tgttatcata
     3781 ctctctggca tactttcaaa gcctttgctc atctctttag tcaatgtatc taactgttcc
     3841 tttggcattg ctgataacaa tttaaatata tcaacatctc cactttgagt agcttgtggt
     3901 ggaagtttac tcattaactg agctttcata gcttttacct ctttactatc gccctcaagc
     3961 cctgatacta ttaaaatagc tttaccaaaa ataacattta aatcatctat agtgtctttg
     4021 tctttagtat ttaactcata aagagattca tcttttagac ctgggtactt atctttatac
     4081 ttaacataat cctttttatt cagcaaagta taattatcta gtactttatc cttactactt
     4141 ttatccataa ataaagtaat tttatttaat tcactctctc taatcacaga tggaacagag
     4201 ttttctactc caccttgttg tatacctaca tttactatat tggatgtgta ttctggtaaa
     4261 gaaaggtcac ataccgcttg aattaccaat agacagacta ttattactat tgataatgcc
     4321 gatttcttta aatatttaat taatttaaac atacatttct tcccttctgt aatattaatt
     4381 agtctaataa cattaaattg aaaaacatta ttttaaactt taaaaatatt tttcatcttc
     4441 atgcttatga catcctgctt ttaaattatc tcgtacttgc attagaagtc ttctaagtac
     4501 tattttttct tcttcactaa atcctaaaaa aaactctctt tccaattcct tgttaaattc
     4561 ttcaacttta ttacatattt ctaaaccttt gtctgtcaaa aaaatcctag atactctttg
     4621 gtccttttca tcttgttttc tttccacaag tccagctttt tccatcctct ttatcataac
     4681 tgtagttgta gatgctttta ctttcctttt atctgctaat tctttttgac tttgcccatc
     4741 tttcatcttt aaagaaaaaa gtatttgggg ttgaccatga tataagccta atttatcaaa
     4801 ataaaaatga gttcttataa aatatattcg cattaattgc aagaaaacat catttaattt
     4861 ttcttcagaa ttgctattca tacttgccct ccttattttt tatattactt agtcagctaa
     4921 gtattatttt actatactgc atgcattttt tcaatatatt tataaaaaat tcatataaat
     4981 ttcatttatt aaatattttt atatcatata gttataattt taataaataa tagtaagatt
     5041 tttaattagt cagctactta tattttttat tgtaaagctt aaaataaacc ttttcatata
     5101 tttttaaata aaaaagttgt cccaaaataa attttgaaac aacatcatat tcaacagtat
     5161 cttgattata taacattatg atactctact tatcttcata tccatttaca acaacatcta
     5221 acttcccagt ttcaggtgat ataattagac catgtacaat aataccatct ggaattaaag
     5281 gatgcccttt tattaaatca atactatctt ttacagattc ttctgcagaa tcgaatccat
     5341 gtaaccattg ttttgtatca attccagcac tacgaagtgt aagtattaca tcattagata
     5401 ttcctctatc taaaatttta ccaagtaact tatccgtatc aacattacac ataccacatc
     5461 catgatgccc tacaaccata acctcatcta catcaaattc gtaaattgca actaaaacac
     5521 ttctcataat acttccaaaa ggatgcatta tagtagctcc tgcatttttt attagtttca
     5581 catctccatt tttcaaattc atagccttag gcaaaagctc agttaatctt gtatccatac
     5641 aagataagac tactattttt ttatttggat gttttgatgt cacatattgt tcatattctt
     5701 tgttctctac aaatgattta ttatattcta atatctcttc aagttttctc atatgtaata
     5761 tcaccctctt taaaaatttt tttat
//
LOCUS       DAKBNN010000220         5770 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000220 DAKBNN010000000
VERSION     DAKBNN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.226"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            131..465
                     /gene="rnpB"
                     /locus_tag="OUB02_003394"
     ncRNA           131..465
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OUB02_003394"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            743..3286
                     /locus_tag="OUB02_003395"
     CDS             743..3286
                     /locus_tag="OUB02_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HCT8887122.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEMEQNEL
                     KEIKQKEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            3409..5400
                     /locus_tag="OUB02_003396"
     CDS             3409..5400
                     /locus_tag="OUB02_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887123.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            5390..5710
                     /locus_tag="OUB02_003397"
     CDS             5390..5710
                     /locus_tag="OUB02_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887124.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
BASE COUNT         2592 a          509 c          999 g         1670 t
ORIGIN      
        1 atatcacctc gagttaaatg tagtttatat atctatatta cactattttt gaataaattt
       61 aaatttttag tataatatta ctttgacatg taaaagaatt tgtgttaaca taactaagta
      121 aaataaatat taagtaagcc agataatcgc atgtaatctt acatgaggaa agtcggagct
      181 ccatagagca gggtgctagg taacgcctag tgggagcgat cctaaggata gtgcaacaga
      241 aataaaccgc cactattgtg gtaaggatgg aaaggcgagg taagagctca ccagcagcta
      301 ggcgactagt tggctatgta aaccccacct ggagcaagac caagatagga ataaagagtg
      361 ctgctcgtac tctccggtag gtaggtcgct tgagcctatt ggtgacaata ggcctagaaa
      421 gatgattatc taatacagaa ctccgcttat agacttgctt aattatttcg atagtcaaaa
      481 acatcagttt aaactgatgt ttttttattt gtaaatttgt ctaatctact tttaaaattt
      541 tgaggaatat tgtaatataa gttagtacag atattaaaaa tggtataata taagatagat
      601 ataaaaagaa aaagtaaaat gatgtaaata atatgatatg atataagatg tagcaaattt
      661 agtaaataat tatttagata tcataatcaa attagcaact ttgcacaatg taaatattac
      721 aataaaataa ttgatttaag gagtgaaggt tttgagtgaa aataatataa taagaaacac
      781 tgttttaaat gtcacagcaa gttttttaaa acaagagtct aaaataaatg aaaagctaga
      841 tggtgttttg gaaaagaaat ttgaaaaagt agaatttaat gaagcaaaat atgcagagct
      901 tttaaaattt aatatattgt tttataagac attagcaaga aatacagaac cacttattgg
      961 caaatggata gtagataagt acatacctga aatagatgaa ttagaaaaag atttagaatt
     1021 aactacagca aaatgtagaa agtatgtaaa taaggctatg aaagagggat tagactctct
     1081 aaaagccaat gatttaaata gttttttagc ttatgataaa atggaactat cagagagaag
     1141 aagacgttta gaaaaggatt ataaggtttt aaacctatat aaagaccttc ttaatatatc
     1201 tcttagaaaa ataagtttag aaaagaaaga atgtggagat ttatttctta aaaatcaagc
     1261 agatgcgaaa agagaattaa aaagagaaat tatattctgt gtaaacaaaa tattagctag
     1321 caataaagat gtggtaccta ataatgtgga agagaataac gaacaagaca aagtagaaat
     1381 aaaagcaact gaaattgagg atttgcaatt aggtaaagat aattcagaaa atgacatgct
     1441 actaaatgaa attaaaaatt caaaagtaaa acatgaatca aaatcaaatg atatagaatc
     1501 gaagtttata gataagttaa atagcttaaa taatgaaagt gtatctcaaa caatacacaa
     1561 tgaatataag aagttatgtg gattagaaga acttcattct gttgaaggat atggttttgg
     1621 caaagaaatt attaaagatt ttgcttgtgc aacggttgta ttagaatttt taaagagaag
     1681 aaatagagac cttatagaag gtgcaatgag actgactata attggagaat ttggtcctga
     1741 aaattttaag gaatttgtgg actatgtaat taaaaataaa acagaaatta gtgaagatac
     1801 atggaataaa gcacagaatc taataaaaga taactatagt gaattagaaa atcatgaaat
     1861 tgcatcaaaa agaactagaa gaaataaaga tatcgatatt gaagaataca tttatatgat
     1921 aaaaaatgca gataaagata tttgctttag aagtagtatt tcaatagaag atgatgctga
     1981 agaaggggtt aaagaagaaa tagatagtaa taatcaagat ataggtgacg ttgtagaaga
     2041 taaagatact acagataaag agtacgacag caataaagaa gatataattg aaccagaaaa
     2101 taaaaagagt aaaaagaagg ctaaattatt tggatttatt aaaaaagaca atgaagaagt
     2161 tgaacaagaa gaagagaatt taaatgatat ttctcctgat ataattttag ataaaccagt
     2221 tgaaaatcat caagtaaaat cagaagaaat ggagcaaaat gaattaaaag aaataaaaca
     2281 aaaagaacca agtcaacata tagaagaaga acgtaatgta aaaatagaaa aaccaataaa
     2341 taataactta gatgaaaaag tatcttcaaa taatgaatcg aagcttgaaa aagaaagtaa
     2401 aaacttagaa gataaaaaag caaaagagat aaaagaagaa aaattagaaa atgaaaaatc
     2461 agtagtaata cctattaaaa agaaagaaaa tagtaataaa aaatctaaaa attcttctaa
     2521 ggacaaatat agagaaaaca agaaagaaat gaaaaattat gtatcagata aagaagattc
     2581 tttagatgat gaagaggttg tttctaagaa gagtagatta aaagaaacta ttatagcagt
     2641 agttatagtg gttattgttg gtgtaggata ttttataaca gtaggcaaca ataagaaaaa
     2701 tgataaagag aatattccaa agtcatcaac tcaacaacaa gctaacaata aattaactga
     2761 agaagagaaa aaagcacaag ctgaaaaaga gaaaaaagaa gcagaagaaa aggcaaaagc
     2821 tgaaaaagag gctcaagaaa aagcacaagc tgaaaagaaa gcaaaagaaa tggaagcata
     2881 taaagatggt aaaggtgtat attacactgt ttatgcaggt tcacttaaag ttgaaaaaac
     2941 agcaaaagaa acagcaaaag aatatgaggc taaaggtatt tcttcaacaa taattcaaga
     3001 aaatggatac tataaaataa aaataggtga ttattctcaa tatggagaag cacaagaaaa
     3061 atgtaatgaa ctagctaaga agtctattga tacatatata gctatgtatg ataagtacta
     3121 tgattataag ttagaagaat taaaagaatc agctccttct ttgagtgctg aagaacttaa
     3181 acaaaaatat gaagatttaa gaagtgagtt aaagaacaaa tctggatatc gtgaatatgt
     3241 taaacattta gataaacttt atgaagaaat agttgaaggt gcttagagca ttataaaaaa
     3301 ggagaaacta tgtctcagaa taaaaagtat taaaaaaaat attatttgtt ttgagacatt
     3361 ttattcacct ttataaaata cttataattc tgtttaggtg gtgaaggtat gttgaaatta
     3421 ggagaaaaaa tcatatatga gctttcagat gggtttgagc ttagtggcat aagtagatac
     3481 tcaaaagaat ataaaaagct tcatataaca aatctaggga atataataat cagcaaatca
     3541 gatgaagtta atgaggatac aggtgtaaaa tatatatata gtttttcaga caataaagaa
     3601 aaattagagg cagttttaga agattctcaa ataattgtct atggaaataa cttgcctttt
     3661 tacataaaaa gtgaaaattc aagtaaaaat gttacagcaa atttgactat gaaaataact
     3721 ttggatgaat atgatttaat ctgcaaaagt caaagagaat ttatattcta tctaaaggat
     3781 aatatgacta tattaagttt agatgacaat aagttttata tgggtggaat taataaagat
     3841 aatgagaagt ttatatttat cagtgggaaa aatagatttg aaataaattt tgatgatata
     3901 gaaagataca ttttagaaga taaaagagtg tcattgaagg gatactttca tatggaaaga
     3961 gaggggatta tagtaaggtc tgtatctata tttaataaca atatagatag tgtagtccca
     4021 tctgatttaa atgagagagt taaagataac caaaaaatag gaaatcttcc taaagattgt
     4081 gaaatagtat tctgcaagat atcaggaaat atagatggat ttgattataa aaacacaaac
     4141 atgcttttag taaaatatca agaccaatta atttttataa ataagaaatc taagaagact
     4201 attgtaaagt cagcgaaaga taattgttct aagttaaatt taggagaaga tataatatta
     4261 tatgataata aaaatgtatt taacctacat ataaatgata aaaatagaga aattatgcaa
     4321 atagatgacc taaaagatat agaaaatgaa atagtgggat atacattaaa acatgctcca
     4381 ttttttattc aagaagattt cgatagcttg actattctaa agtcttttca aaaagaaatc
     4441 atatctataa aaaatagtga tataaaagac attgtaatta ataaagaact tgaaaacgaa
     4501 aattctaatt ttgtagaaac ggaaataaaa tttaataatc aaaaagtact tttaaactta
     4561 agtaaaagta tggttcaaaa acttatgcaa gatgtattta tatatgcaaa gcaacctcta
     4621 cttaaagaaa attctattga agtgatatat aagaattggt ctaaagctat gaatgatatg
     4681 ataatattta atttctttgg aaatatttac tatatgaaat ctgaatttga taagatttta
     4741 gaaaaagagt taaatgatga aataagaata gaagttataa attcacttta taagcaaata
     4801 caagaacaaa gaaacaattt agatttattg tcagcatata tgccaagaat attagaaaat
     4861 caagaaatag atttgtttga aaagtataat actaaacttg atgtacaagt gtttaagcag
     4921 attaaaaatc tattgtctga tttatcatat aatataagca gttatttaaa tgaagttgaa
     4981 aaaagtttag acaatatcat ctttgtaatt tcaggagaag ataagaagaa atacaattat
     5041 agaatgttaa aagaaagtga aagtgcaagt cttgatgtat ttttaaaaca agccatatca
     5101 agattaaacc atttagtaga aaatatgtat ccttattatg tagatgaaac tagtagggaa
     5161 atgtttaagt tatttgagtt attgtggaaa aactatagaa atattgatga tgatagtata
     5221 aaagaaatat tattcgagag aataacaaat acatatgtat ttaaacaatt gacattaaat
     5281 aatagtacaa aagaaagaag aaaagacata attgaaaaaa tatataactc tgttgattat
     5341 ggaaccaata aactagatga aaatatgttt ttcacaggag gaataaagta tgtcaagtaa
     5401 atacaatgtt tggactttta actatgagtt cttaggattg gactcagggg atgaaaaaaa
     5461 taatgaagtt tattatgaga ttgatttaga tgaaatttta aatgaaaact atatagatga
     5521 agatatagat agtggtttag attctgatgg aaatagtatt ttaggagaat taatatcaga
     5581 aatggatata gatgaggatg tcagtgttga tatgacatat caagaactag aagttgactt
     5641 agaagattta gattcttatc tagatgaaga tatagatagt gatataaaag gcatcttaga
     5701 tgaaatttag tacctgtata agttttggat aaaatataaa caatacaata ttaacttata
     5761 aaactaaagg
//
LOCUS       DAKBNN010000221         5740 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000221 DAKBNN010000000
VERSION     DAKBNN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.227"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..302
                     /locus_tag="OUB02_003398"
     CDS             <1..302
                     /locus_tag="OUB02_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HCT8887125.1"
                     /translation="CKILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLR
                     NDILTCSKAKVSKYITHELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            295..894
                     /locus_tag="OUB02_003399"
     CDS             295..894
                     /locus_tag="OUB02_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HCT8887126.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            1054..1932
                     /gene="glyQ"
                     /locus_tag="OUB02_003400"
     CDS             1054..1932
                     /gene="glyQ"
                     /locus_tag="OUB02_003400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCT8887127.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            1932..3998
                     /locus_tag="OUB02_003401"
     CDS             1932..3998
                     /locus_tag="OUB02_003401"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCT8887128.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMSFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(4164..5711)
                     /locus_tag="OUB02_003402"
     CDS             complement(4164..5711)
                     /locus_tag="OUB02_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HCT8887129.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT         2179 a          679 c          977 g         1905 t
ORIGIN      
        1 aatgtaaaat tttacaaaat tctgtgttta atatctacga aggtggtata ctatgtagta
       61 agtgtagtaa actttttgat aataatataa aattagactt aacaactata agtcttatgg
      121 aatatgtatt aagaaatgat attttaacat gtagtaaagc taaagtatca aaatatataa
      181 cacatgaact tgagaatatt ctagacaagt atttgaaagt ttatgttgat aatattaatt
      241 ttaaatcatt acacgtattg caaagtgtaa aaaataataa gggagtggat aatgatgagt
      301 agtaatataa ctattgaaat ggttgattca gtctttgaaa gagttccagg agcaacttat
      361 gcagaagcaa aagaagcatt agtaatgtgc aatggagatg taattgaggc agtaatatat
      421 ttagaaaata aaaaaaatac ttgtaattgt aaagcaaaat ctgctaaaga aactatggaa
      481 gaagtatttg gcaaagatgg agaaaatata aagtttcaat taaaagagct tcttagaaaa
      541 agtagtgtag tgagaataat agttgaaaaa gatggaaaaa ctatgatgaa cattccttta
      601 acagttggag ttgttggatt agcttttgta ccattagcta ctttagttgg attatcagct
      661 gctgttataa gtaaatatcg tataaaagtt caaaatgaag aagatggaga aattgtagat
      721 ttaggtgaat tgaatgaaga aaaacttcac gttttgaaga atatgataac taatgcagca
      781 aaagatgtga aggatgtagt agttgacaaa aaagaagatg acaaagatgt aactgctgac
      841 cttatgaaag aagaatctga aaagaataaa gaagacgaag acgaaaataa atagatataa
      901 agtgactttt aaaaaatgaa gagaagataa ttataagaat tatcactaac attgacaaat
      961 tttatagaat ttgatattct aaaagttgta gaagaactat taaaatagcc tttcgtgtag
     1021 acgagaggct ttattgttat aggagggatt tttatgaatt ttcaagagat gatactggcg
     1081 ttgcaaaaat actggtcaaa gcaaggttgt atcatgatgc aaccttatga tattgaaaaa
     1141 ggtgcaggaa ctatgaatcc taatactttt ttaagatcat taggacctga gccttggcaa
     1201 gtatgttatg tagagccttc aagaagacca gcagatggaa gatatggtga aaacccaaac
     1261 agactatacc aacatcatca attccaagtt atactaaaac cttctccaga taatatacaa
     1321 gaattatatt tggaaagttt aaaagagatt ggaattgacc caagtgagca tgacataaga
     1381 tttgttgaag ataactggga agctgctaca gttggtgctt ggggacttgg atgggaagtt
     1441 tggctagatg gtatggaaat aactcaattt acgtattttc aacaagtagg taatattgaa
     1501 tgtgagcttg agacaggaga aattacatat ggattagaaa gattagctat gtacattcaa
     1561 gaagttgata gtgtttatga tttaaaatgg aatgataaaa taacttatgg tgaagtattt
     1621 aataaagctg agtatgaaaa ctcaatgtac gcatttgaat tatgtgatgc agatatgttg
     1681 tttaatctgt ttgatatata tgaaaaagaa gcattaagac ttatggaaaa tggacttgta
     1741 ataccttcat atgactatgt gttaaaatgc tctcatgcat ttaatacatt ggatgcaaga
     1801 ggtgcaatag gagttagtca aagagcttca tttataggta gagttagaaa tatggcaaag
     1861 actgttgcag aaacttttgt aaaacaaaga gaagaaatgg gattccctct tttaaaggac
     1921 ggtgacaaat aatgaaaaat tatcttttat ttgaaatcgg tgttgaagaa atgccatcaa
     1981 gatttgtagg cagtacacta gaacaattaa aaaacaatct tagtaaatta tttgatgaaa
     2041 atagaatagc ttttgataaa ataaatgcgt atgggacacc aagaagactt actttagtag
     2101 tcgaaggatt gagtgataga caaaatgatt tagaagagga aataaagggt cctgctaaga
     2161 aaattgctgt tgatgcagaa aataatttaa caaaaccagc attaggattt ataaaaagca
     2221 aaggattaag cgaatctgac atatatttta aacaagttgg aaaagaagaa tatatatttg
     2281 cgactataaa gcaagatgga aaagaaactt cagaagtact aaaagacata ttaccagaga
     2341 ctataaaatc tgtagtattc ccaaaagcta tgcgttgggg aggaaaaaat cttaaatttg
     2401 caagacctat aagatggata gttacattgt taaatgataa agtattagag tttgatttag
     2461 aaggaataat atcttctaat gtaacaaaag gtcatagatt tttaggtgaa agtacaatag
     2521 aagttgattc tatagaagat tattttgaaa aattagaaaa aaattatata gttttagacc
     2581 aaaacaagag aaaagaaatg ataagaaaac aatgtataga agtagcaaat tctttaggtg
     2641 gagaagttga atttgatgaa gatttacttg aagaggtaac ttatttagtt gaatatccaa
     2701 ctgcttttta tggagaattt gatgtggatt atataaaact accaaaagaa gttgttataa
     2761 ctcctatgaa acaacatcaa agatattttc cagtacttaa agaaggaaaa ctgttaccta
     2821 actttatagc tgttagaaat ggtgattctt acagaataga caatgttaaa gctggaaatg
     2881 aaaaagtttt agaagcaaga cttgcagatg ctttattttt ctatagagaa gatactaaga
     2941 gaaacttaga aagctacata gaaaaattaa aaactgttgt attccaagta aaactaggaa
     3001 ctatttacga caaaacttta agacttgaaa aattaagtgc tgatatactt gatagtttag
     3061 gtttagtaga tgaaaaggca gatactataa gggctgctaa attatctaag gcagatttag
     3121 ttactggaat ggtaggagaa tttgatgaat tacaaggttt tatgggaaaa gaatacgcaa
     3181 aagttggagg agagaatgat gttgtaagtg aagctatttt tgaacattat cttccaagat
     3241 ttgcaggaga tatacttcct aagactaatg caggggttgc cttatcaata gctgataagt
     3301 tggattcaat agcaggtttc tttgctatag gaatacaacc aacaggttct caagacccat
     3361 atgctttaag aagacaagct ttaggtatac ttagtatact tatggacaga aaagttgctg
     3421 tggatataaa agtacttatt gaacatgctt tagataatta ttctgaatta gattttaata
     3481 aagaagaagt tgttgagcaa ataatgagtt tcttcaatga gagagtaaaa aatctattta
     3541 aagatttagg aataagatat gatgttgttg atgctgtttt aagttctgat ataaatgatg
     3601 tttctgatat gcatatgaga gctttagagc ttaataattg gctagaaaaa gatgaattag
     3661 tggagatgct tactgcattt aatagagttt ctactctagc tcaaaaagca gaaagtgata
     3721 aaattgatga atctttatta aaagaagatg ctgaaattaa actttacaat gaatttaaac
     3781 aagtaaaggt aaaagttaat gaattattaa aggataagaa atatggtatt gctttagatg
     3841 aatttgcatc tttaagacca gtagttgata atttatttga tacagttatg gttatggata
     3901 atgatgaagc tattaagaac aatagacttg ctttacttaa acaagtatat gatactatgc
     3961 tagaaatttg tgatttatct aaaattgttt ataaataaaa tttaaactat ttataagaaa
     4021 taaggctgtc tcaaaataga gatagtctta tttttcattg ttgagatgat gaaaatatat
     4081 ttgatttcat agaaaaagag tgtctcataa actaaaaagt tcattttgag acacccatgt
     4141 tttttgtaag taaatataat aaattatatt ttatacttat cagttatttt gtttaaaata
     4201 ttatcatttg aatttaccca ttcataaaaa ttgcctgatg atgcgtccga tattttataa
     4261 agcatttcag gaatatcact agctttaaaa tctccaagtg attttcccaa tctagtctta
     4321 cctatttcaa aactgcctcc aacaaataat tcaaatgcat ctgtagatat accatcaaca
     4381 ttcttctttt taccacataa acctatgcta ccaacttgat gaacaccaca tgaatttaaa
     4441 catcctgata tatataactt aggtgcttta tttaaaattt cttcattatt ttgtaatcta
     4501 aaataatcta tgatttcatg taacattttc tgactatttt gtatgcccat ttgacaaatt
     4561 ggaacaccta tgcaagatat actttgttct aattgagaat tacaactaat agttttactt
     4621 atttctaata ttctttttgc ttcatttcca tttaaattta aaatatataa tccctctgtc
     4681 attcctagtc taatcatagg attttcaaca ttatctaatt ctttaagtaa agtacttaaa
     4741 tcctttgtta acaaaatacc tccaactgga tgtatatata cagtgtataa accatcctgt
     4801 ttttgaggta ttagtctaga atcttgtata tctattttaa ctccaggttt agagtaatct
     4861 atttcttcaa tattgagttc aagactacca ttttcttttt ctaatttata gtattcttta
     4921 aatttttcta agaatagttc ttcaccaagt gattcgacca tatatctaac cctagctcta
     4981 tttttatttt cataatttcc ataatccata aagaatttaa tcataccttc aacacaatat
     5041 aaggcgtctt tcggctttat aagttcatca agttctaaag caactttggg ctgtttacct
     5101 agacctccac ctaagaaaac tctgaaatat ggttcatcat ttttcaaagt agcaacaaat
     5161 cctaagtctt gtatactaca atgagcagta tctgtgtaac aattagaata agaaactttt
     5221 aattttctag gtaaatggta tgtagtagca tttttgataa aatatttatc agtagcaact
     5281 gcatatggag taacatcaaa gacttcacta gggtcaactc cagataatgg agataatccg
     5341 acatttctag gataattacc accaccacct ctagtgaata tatcattttt tattccttct
     5401 tccatgatat caacaattga atttatagat aaatcgtgta attgtatagc ttgtctagtt
     5461 gttaagtgta tcttatctaa gttgtgttta atagctattt catagatttt attaagttga
     5521 tactgagata aaactccagc agatactcta agtcttatca taaaagattt tttgtctctt
     5581 tgagcataaa caccatatcc accagaaaat cccttatatt gcattttatt tatttctcca
     5641 tttaaaaatt ttgaagaata ttctttaaaa ttaggtagtt catttaacaa tatcttttta
     5701 aaattatcca ttatttcacc tcattgttag tcttgtataa
//
LOCUS       DAKBNN010000222         5660 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000222 DAKBNN010000000
VERSION     DAKBNN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.228"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(140..2359)
                     /locus_tag="OUB02_003403"
     CDS             complement(140..2359)
                     /locus_tag="OUB02_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCT8887130.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(2635..5289)
                     /gene="adhE"
                     /locus_tag="OUB02_003404"
                     /gene_synonym="adhC"
     CDS             complement(2635..5289)
                     /gene="adhE"
                     /locus_tag="OUB02_003404"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCT8887131.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KGTKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1869 a          997 c          593 g         2201 t
ORIGIN      
        1 taatttttat aaaatacaat tattttttgc tttatattag acaatacatt gataaaataa
       61 aaaaacacta attatttagc aatgatttat taaaatcaag cattcataat agtgctttat
      121 ataaaaatca aattttgttt taaagtaaat ctaaaaaatt cttagctggc attggtcgtg
      181 caaaataaaa tccttgaact aaatcacatc caaattgttt cattaactca gcttgttctt
      241 tactctcaac accttctata attacaacta aatctaggga ttttgctaga gacactattg
      301 attttgctat attgcgactt ctctcatcat gttcaatgcc accaagaaaa gacttatcta
      361 gcttcaatat atcaattggc atatctctta aacaactaat agaagaatat cctgtaccaa
      421 agtcatccat agaaatagaa aatccatacg tcctcaactc atttaaaaca ttctttaaaa
      481 tatttaactc attaatagcc acagtttctg ttatctctat ttcaatgaaa tctgaactta
      541 aattatacat ttttattgtt ttattgataa attttacaat atctggttga tacaactcga
      601 ccctagataa atttactgaa actggaacta tctttttacc ttgctttttc cattcatata
      661 aatcattgca aaccctttca aatacaaatc tcccaatttt taaaatcaac ttacttttct
      721 ctgctattgg aataaatact gcgggactta taaaaccatg ttctggatga ttccatctta
      781 ttagtgcttc actccctatc atttctccag ttacaagtga aaacttaggt tgataataca
      841 cttcaaattg attttttacc aatgctattt ttatatcatc taaaatcatt gattcctcac
      901 taagcttatt cctaacatct tcattataaa tagcataatt tatattttta cctttagcaa
      961 cagacctagc catatttgct ttatttacag ccttttgaat atctacttca ccttcttcaa
     1021 caaaataaat acccacacac agagaaattt caaagactat actcaaatta ctttcaatat
     1081 tacttcgtat attatcaagt tttcttataa ttttatttct tccattttta taatcacaga
     1141 aaattgcaaa ataatcactt gaaagtcgtg catatacctc accttctgtt aattcttctt
     1201 ttaaagcttt tgctattgta attaataaat tgtcaccaaa ttcatatcca aaagtatcat
     1261 taatcatctt aaagttatta atatcaaaat atactataat ataatttaaa gatggtttat
     1321 ctaaaagata cttactacat tctctataaa atttattttg atttcctata tatgtaactt
     1381 tatcttcata agccaaactt aatatttctt tttcattaga tttttttata taaattatat
     1441 aaaaaataat tgcaataaat attaatacaa tagataacac tgcatataca gctctgttga
     1501 ttatttgttt tgaattacta aatacaacag tttctggaat tgaaaaaatt aaattccagt
     1561 cttttatata attattttca ctattcccat tatccatagt ggcatatcct aaatatcttc
     1621 tttcttccaa catatttaat acagttgcac ctgttttatt ttcttgcaat tcttttttta
     1681 atctatttac ttcttgaata tcattatcct gttttaacaa tttataaata ttttttgata
     1741 ggctatcccc ttgtggatga aggataattg taccattatt ttttacaaca taagatatac
     1801 tattattttc ataaaaagat atattaataa gtttcatcag agtattgata ttgtaagtac
     1861 aatataaaac tccaactact ttatcattat ttaataatgg tactgaaata gcaatcttct
     1921 ttatagactt atttacttca tcaacatatg gacttgaaac atacttctta cctttaattg
     1981 agtttttgaa aaattctcta tctttcactg aaaacttatc acctttggta ttttcagcat
     2041 taccatttat atcaactatt gccatagtgt aaaatttttg attttccatt tcatctaaaa
     2101 gatttttcat tttttcctca ttaggtatat ctggatttga agcataatag ttagaaaaaa
     2161 tatttaaaac atctaaatct ttttcaattt gtctttgtgc tagttgagag ccttgtactg
     2221 caacttcttt tatatgtatt tttgcctctg acataagaag atgttttgta tcttctatat
     2281 agatataccc cattgtcaaa aataatgatg ttaaaaaaag aattgtaatt aatgtaaaac
     2341 ttttatttct ccctgtcatt cgactcctcc cctaattagt caatacttca tatagataat
     2401 atatcctttt tataccaatt catcatttat attgtatata actattctta aatgttacac
     2461 ataaaatcaa cataataacc ataaaaacat tcttttatta ttataataac actatttaat
     2521 tgaattttaa attaaagaat aaaaactatc cattttctca aacaaaaaaa ctcttataca
     2581 ataaaacttt gttttatgta taagagtttt tattttatct attttatagc aaacttatct
     2641 tccataatat gaatttaaat acatatcttt tatttcattc ataagtggat atcttggatt
     2701 tgctcctgta cattggtcat caaatgcttg aattaccatt tcatctattg aatccataaa
     2761 cttagattct tcaacgccaa actcttttat agatttagga ataccaactt taagttttaa
     2821 atcttcaata gctaccaata aactttggaa tttctcttca tcagaatttc cttttaatcc
     2881 taaaaatgaa gctatttcag catatctctt taatgcttct ggatatttat attgagagaa
     2941 tgctcccatt ttctttggtg catctgaaga attaaatttc ataacttctg ttatcaaaag
     3001 agcatttgca acaccatgtg gtacatggtg gaaagcacct aatttatgag ccatagagtg
     3061 acaaacaccc aaaaatgcat ttgcaaaagc catacctgcc attgtagaag catttgccat
     3121 tttttctcta gcttctgggt cttttgcacc attatcatat gcacgtggaa gatattcaaa
     3181 tatactctta atagcttgta acgctaatcc atttgtataa tcggttgcaa gcatagatac
     3241 atatgcttct aatgcatgag ttaaagcatc tataccagaa gcagatgtta agctttttgg
     3301 catattcatc atcatatcag aatcaactat agccatattt ggcatcagtt cataatctgc
     3361 taatggatat tttactcctg tatcttggtc agttataaca gcaaatggtg ttacttctga
     3421 accagtacct gctgatgttg gtatagctac aaaattagct ttttcaccca ttttagggaa
     3481 tgtatatacc ctttttctta tatccataaa tctcatagct aggtcttgga aatctacatc
     3541 tggatgctca tacaatgtcc acattatttt tcctgcatcc attgcacttc ctccacctat
     3601 agatattatt acatctggat taaattctct catagcttta gcgcctatct tagcacattc
     3661 caaagttggg tctggttcta cttcaaagaa tgtagtgtgc ttaatattca tttcatctaa
     3721 taaatcagta actgctttag tatatccatt attgtaaagg aatgtatctg ttacaataaa
     3781 agctctcttc ttattcatta catctttaag ttcttttaat gctactccta aacaaccttt
     3841 tttgaaataa actttttctg gtgctctaaa ccaaagcata ttttctctcc tctcagctac
     3901 tgttttaata ttaagtaaat gcttaactcc tacattttca gatactgaat ttccacccca
     3961 agacccacat cctaaagtaa gagaaggagc tagtttaaaa ttatataagt cccctatacc
     4021 accttgagaa gaaggagtat taattaaaat tctacaagtt ttcatggctg aagtaaactt
     4081 atcaagtttt tctctttgat ttacatcatc aatatataat gaagatgtat gcccaaatcc
     4141 accatcttct actaattttt ctgcttttct aactgcatca tcaaaatcac ttgccttgta
     4201 cattgcaagt actggtgaaa gtttctcatg agcaaaagct tcttctattt caactgattc
     4261 tacttcacct attaaaacct tagtatctac tgggacttca aagcctgcaa gttttgctat
     4321 agtacaagct gtttgcccaa ctattttaga atttaatcct ccatttacta aaattatatt
     4381 tcttattttt tctgtttcat ctttatctaa taaataagca ccacgctctt taaattcttt
     4441 tttgacttca ttgtatatat tttctaatac tataaccgat tgttcagaag cacatatcat
     4501 accattgtca aaagtcttag atacaagaat tgagtttaca gcagttttta tatctgcact
     4561 atcatcaatt attgctggag tattacctgc accaactcca atagctggtt ttccagatga
     4621 atacgctgat ttaaccattc caggacctcc tgtagcaagg gtcaaatcca cttctttcat
     4681 aacagttgta gttaattcta aagatggctc atctatccat cctattatac cttcaggagc
     4741 tccagctaaa acagcagcct caagaacaac ttttgcagcc tctatagtag aattttttgc
     4801 tcttggatgt ggagagaaaa ttattccatt tctagttttt aaagctaaaa gtgctttaaa
     4861 gatagcagta gaagttggat tagttgtagg tacaactgct gcaataacac ctattggctc
     4921 agcaatttta gtcataccaa atgcttcgtc tttctctata acaccacaag ttttagttcc
     4981 tttatatgca ttatatatat actctgaggc ataatgattc tttataacct tatcttcagc
     5041 aattcccata ccagtttctt tttgtgccat tatagctaga ggaactcttt gcttattagc
     5101 tgccaaagat gctgctaaaa aaattttatc tacctgctct tgactatatg tagcaaatat
     5161 tttttgagct tctctgattt ttgctagttt tttaactaag ctttcaacgt tatttacagt
     5221 atctaaacca actgacataa cttcattttc ttttttattt tctattatta cttgttcttt
     5281 atgagccatt ttaacaacct cctgagatag ttttttattt atcatgttaa tttattaaca
     5341 ttatagcatt tactttttct attttcaagt tattttttta acattttatt ttttatctaa
     5401 tataacacca tttctactaa taatatctag ctttacaaat acttaattta actagatatc
     5461 actttatttt ttacgcaaat cactaatgaa atcatctaaa aacttaagga taacgttatt
     5521 tttttaacgt ttatattttt gtatttttta caaataaaac tttataaaaa gaatttttaa
     5581 tatgatactc tatcaagttt aaaaataaaa atacttgaaa atgaaaataa aaacttaatt
     5641 aacaagttaa agtaaatata
//
LOCUS       DAKBNN010000223         5603 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000223 DAKBNN010000000
VERSION     DAKBNN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.229"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            120..1739
                     /locus_tag="OUB02_003405"
     CDS             120..1739
                     /locus_tag="OUB02_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCT8887132.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2113..2307
                     /locus_tag="OUB02_003406"
     CDS             2113..2307
                     /locus_tag="OUB02_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8887133.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2866..3189)
                     /locus_tag="OUB02_003407"
     CDS             complement(2866..3189)
                     /locus_tag="OUB02_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887134.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3234..3488)
                     /locus_tag="OUB02_003408"
     CDS             complement(3234..3488)
                     /locus_tag="OUB02_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887135.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3752..4033)
                     /locus_tag="OUB02_003409"
     CDS             complement(3752..4033)
                     /locus_tag="OUB02_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887136.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDIVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            4346..5155
                     /locus_tag="OUB02_003410"
     CDS             4346..5155
                     /locus_tag="OUB02_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887137.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            5197..5463
                     /locus_tag="OUB02_003411"
     CDS             5197..5463
                     /locus_tag="OUB02_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HCT8887138.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT         2067 a          705 c          686 g         2145 t
ORIGIN      
        1 aacattaata tttttattga caaaatttgt tattctatat tatggaaaca ataaatttta
       61 gttaataaag tttataattc agataaaaaa attattaatt tcttaaggga ggaaatatcg
      121 tgaatatagt aattagcctt atcggtggac ttggactttt tctttacgga atgagtttaa
      181 tgggagaagg tttacaaaaa tcagcaggag acaaattaaa aaaaattatt gaactcttaa
      241 caagtaatgt tgttatgggt gtactggtgg gtacagtcgt tacaggtatt atacaaagtt
      301 caagtgctac tactgttatg gttgtaggat ttgtaaatgc aggtataatg aatctaagtc
      361 aagctattgg tgtaataatg ggggcaaata taggtactac agttactgct caattagttt
      421 catttaatct tgaaggaatt gcaccaattg ctttaggtat aggtatttta ttctatttat
      481 ttacatcaaa tcaaaaaaca aagcatttat ctgagatact tataggtttc ggtatattat
      541 ttacaggaat ggagtttatg aaagatgcag tagctccact tgctgaatac aaagcattca
      601 ctgatgcact tttatatttt agtaaaaatc ctgtattagg tatattggca ggatttgcca
      661 taactggtat tgttcaaagt tcaagtgcat ctatgggtat gttaatagca ctagcttctc
      721 aaggaatact tcctctatca tctgctttac ctattcttta tggagataat ataggtactt
      781 gtgttacttc acttttatca agtgttggtg caagtagaaa tgctcgacgt gcagctgtaa
      841 tgcatttaag ttttaatgta attggtacaa taatatttat gcttgtttta aataaaccta
      901 tatcagccat agttactcat tttgacccaa ctgatacagc tagacaaata gcaaatgcac
      961 atactttatt taatcttaca aatgttataa tattattacc attttctaaa tacatagtta
     1021 aattagcaaa tagacttata cctattaaag aaactgaatc agaaatagta aataatacta
     1081 agtacttaga tgaaaggatg tttagcactc catctatagc acttggtaat actgtacaag
     1141 aagttgtaag aatgggtcat aaagctacaa actctttgga acattcaatt gcaggatttt
     1201 taaataagag caatgaagat attaataaag cttttgagtc agaaaaagtt gtcaataagt
     1261 tacagaaaga tatattgaac tatttgttaa aactttctaa agaacctctt agagatgatg
     1321 aacgctttag aacagatttg ctttttaata ctgtaaatga cattgaaaga gtctctgacc
     1381 atgcagaaaa tatagctgaa ttggccatga gtgttaagga aatgaacatt agtttttctg
     1441 atagtgcgat aagagaaata tacgaaatat acaataagac aattactaat tttaaggatg
     1501 ccttaatagt tttagatgtt aaagattttg agttagcaaa taaagtttta gaagttgaaa
     1561 atgaagtaaa ttatttagaa aaaactttta gaaattcaca tatgattaga ctaaataatg
     1621 gaagttgtac aattgatgct ggtgtacttt atcttgactt acttactaat ctagaaagaa
     1681 tttctgacca ctcaacaaat atagtaaaac aagtactaaa acttaaacaa aaaatatagt
     1741 gattattttt atatataatc ttaatcaatg agaagtaaga agctatagat aatgcttata
     1801 tcatttatgg cttcttattt tttatgtaat actcaaattt ttatagtatt ctcttattat
     1861 taaacagttt ctgcctagaa tattccatat atatatgact tctttcataa aacttgaact
     1921 gcccataaat aaagtcacaa gtatacataa ctatttttta atcaattata accaatattt
     1981 gttttattta tcttcatttt aattcttcat tcaataatgt aataagatta ttttttattt
     2041 ggatactatt atatcatcta aatatgttat tatatatttg gatattttca tattaatagg
     2101 agggattcta caatgataaa ttatatggta gaaattatat atagcgaaaa aataagcgaa
     2161 gtagaaaaca tgttgaaaat tgaaaatata caatatgaat ttacttctcg tgatggcttt
     2221 atattttttg gtttttatga tatgacagac gcatatagat ttgagacttt atgttcagat
     2281 gaaaaaattc gatttaaaag aatataatag ataaaaaagt acatatttct aaactatttg
     2341 tttttcaaat ttaggttgtg ttttaatgta gtgttgatat tataaacaat tttaataata
     2401 aaaaagtagg gacattatat taatatgctc ctacttttta ctcatactta ttaatattat
     2461 ttatcaaaat taccctacaa actggaattt atcattcttt cataatttta aactatcata
     2521 tttaaaatat ataaaattct acatgatttt tgtataaaca taatatcaac cattatccaa
     2581 tttaaaaata ttttttagta atttccattt cattttctct taacatatta attaaacttt
     2641 tatatgtaaa tggtgtattt acatattatt taatatccaa caattatata aagaaatgtg
     2701 ctctttgcct tataaagaat tataaagcca attcgtttta ttatattatt tttctttata
     2761 ccttaaaaac tttaccttca aattcttatt tatctatata ttataaaaat gttcttaacc
     2821 tcttttacga gattttcttg acatattatc agttagataa aataattaaa taatataatc
     2881 agcaagtatg ttttttactc ttttaagtaa atttccacct gcaccccaat caatttttaa
     2941 aattccaatt gcactgcctg ttgaagtagc tttaataact gtttttcctg tggtattttc
     3001 ataaactact attaatcctg catcactatt atttcttgca taatacttct caaaagttgt
     3061 aattagtata aatttatcat ctcctaaatt ataagaatca ctaaaaacta aatctgcatc
     3121 aagattttct tttatcattt tttgtgcctc taagggtgta atttttacag taaatatttc
     3181 tgttgccatt ttttatctcc tcataagaat ttattttaga caaaacataa tctctagtat
     3241 tttctgatat atttatcaaa caatatccct attcctccta acagagctaa tatagaacca
     3301 aaatttctat attgagaaat agattggtct agataaatat tatattggtc agtatccatg
     3361 attccaccat tttgttgaat aatatgctct gcactaaatt cacctaaacc tatacaacag
     3421 aataatacta ctactccaat tacaaaagca aatatacata ttgttaattt aaaatcctta
     3481 ttttccatag ttatccctct cataagctaa atttatttga ttatttttac tagattttat
     3541 attactctta ttatacaata caacatatat ttacatctat ttccaaaatt gtaaccttta
     3601 tagttttata tatttaaatt ttttaaacaa aaaatacaca tctataaatt ataaatgtgt
     3661 attctgtacc attactaata atatattata cttttttaca aatttctaat tatttataac
     3721 tattcaaaaa taaaattcat ataaaataat tctattgtac aattgaaact ttaataatgt
     3781 ctccattttt tctctccaag tgaactactt ctccagttaa atcatagtca aatgctttaa
     3841 ctaatatttc agtaactttt ttaccatcta ctatatcatc ttttccatta aataaatcca
     3901 aagcattatt ctcaatcatt ctctttgcaa aatttacagg taactttata tgtattttat
     3961 cgcctgtatg tgataagact cttattctta aaaacttttc taaattgttt cttggtgttt
     4021 gttttgatgc catgctatta aatcactctc ctttattttt tatttaataa taaattttat
     4081 tcttaaaaat atctttatat gtatttatta tgaaagtaac actattattt tctaatcttg
     4141 ttttatattc cttcttcttt aatatattta tttaataaat atctatgatt tataataatt
     4201 gtatattttg agattttact ttacaattat tgtatctaaa tttaacaaaa tatcttatta
     4261 gaatatttca attaaaacaa aactggttta ttatataaaa aataaattag tatacttatt
     4321 ttttatatat caggaggata tacttatgaa taaaataatc aataacattt taaatacctg
     4381 caaacagtat acatatattt tgccacataa attgttaaga cagtatatag cacactatac
     4441 aatatctatt ccagatacaa gtattaaaga aaatcttaca ttaatacctg atgctagtgg
     4501 atgtatgata tttaaatttg acaaaaaagg tatagaaagc gcattctggg gagccacaac
     4561 aaaaactact attgtaaaaa atgatataga aaatgtactt tttagagtat tcgttgagtt
     4621 tcgcccaggt ggagtatatt atttgacagg tctttctcaa agagaatcta cagatttaaa
     4681 aatttcactt gaagatttta acacattatt tagcttagag gttaattcca tttttgaaag
     4741 aacaagtact ataaaggaat tagtagaaca attagatatg ttattcttat catacttatt
     4801 gaaatcaaat atagtagata tgactatacc aattcttgaa aatgctagaa aacaaaactc
     4861 tattatgtct gttaaaaaca tttcacaaat tagttgttat agtgaacgac atttaaatag
     4921 aatttttaat aattctcttg gtatgagtgt taaatcatac cttagattac ttcgtataaa
     4981 ccttgtatta caagaaatac aaaataataa aataccattt gcaactttag cacaagatat
     5041 tggttattac gaccaatctc attttataaa tgattttaaa tctatttgtg gtgttaatcc
     5101 aacaacctat ataaaaaatt tgtccgattt ttacaatgaa aagtataaat tttgatatac
     5161 aatcatataa aatttatatt taaggagtga cttaaaatga tagaatcaag atgtggaatt
     5221 ttatgttatg aatgtggata caaggaaaaa gtcaattgta aaggttgtac agagattgag
     5281 aaaccatttt ggggtgaaca atgtcctgta aaatcttgtt gtgaagataa aaaccttaca
     5341 cattgtggtc tatgtgatac atttccatgt gaaatgctta atcaatttgc atatgacaaa
     5401 gaacaaggtg atgatggcaa aagaatttct cagtgcaaaa aatgggctgt actagcctta
     5461 taatatactc tcttgttata aaataagttt ttgtaaggta aattttttaa actgttgtag
     5521 aaatttctgc aacagttttt ataattaaat ttaaaggtta tttaattaaa ttaatagaat
     5581 agtaatttat tgataaaatc tga
//
LOCUS       DAKBNN010000224         5603 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000224 DAKBNN010000000
VERSION     DAKBNN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.230"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            91..1305
                     /locus_tag="OUB02_003412"
     CDS             91..1305
                     /locus_tag="OUB02_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887139.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(1724..2245)
                     /locus_tag="OUB02_003413"
     CDS             complement(1724..2245)
                     /locus_tag="OUB02_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887140.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYNKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            2601..3398
                     /locus_tag="OUB02_003414"
     CDS             2601..3398
                     /locus_tag="OUB02_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HCT8887141.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            3508..4098
                     /locus_tag="OUB02_003415"
     CDS             3508..4098
                     /locus_tag="OUB02_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCT8887142.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNKIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMAYIFINSINKYIEEL"
     gene            4164..>5603
                     /locus_tag="OUB02_003416"
     CDS             4164..>5603
                     /locus_tag="OUB02_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCT8887143.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFR"
BASE COUNT         2270 a          574 c          949 g         1810 t
ORIGIN      
        1 ttacagacat tttgttaact ttccctcgat ttactagtaa atattttttt aatatactta
       61 aattacaaca gatgtactag ggaggtatat atgcaaatta aaataggtaa agtcatacag
      121 cgtcttagaa aagaacgaaa tttaactcaa gaacaattag caaagtttat aggtgtgtca
      181 acaccagcag tatctaagtg ggagagtgga aattcttacc cagatataga attattgcct
      241 ttattagcag acttttttaa tgtgtctatt gataagcttt taaattacaa gattgattta
      301 agtgaagaag aagtaatgaa aatttataaa gaattagaat caggctttgc tagaattgaa
      361 attgatttat caacagaaga accaaaagaa gaatttagac aagacttaga atctgtaaaa
      421 aagctttcca atatgtatat agagaagtat cctaaaagct atttattgaa gctaaggata
      481 tgttcgctgt atcagatgta ttcatataaa tttggtaaga gtgaacttaa tgacagagta
      541 aaagaaacaa cgaatattct tgaagatata gtgagaaata cagatgatat ccaaataaaa
      601 gaaacagctc ttattatatt atcaaacgca tattgtatgt tggaggatta tgaaaaggca
      661 gagttgtatt taaatatgat acataagtca ataggggata caagtgttaa tttagctatg
      721 atatatttaa agcaaaatag acttgaagaa gcagaaatat tgcttcaaaa taaattgttt
      781 agtaatgtat ttaatataag catggattgt aagggaataa ttaatgtata caaaaatcaa
      841 tacaaggaat taaagaaaaa attggagaat agaaatttta ataaaaatgc aattgagaga
      901 gaaatggagt atataaaaaa taagttgctg gggtatgcaa atctttcttt agaaataaaa
      961 aaaatgctca gtgaagataa aggtgcattt tttagtatgt atatggatta tatggaatta
     1021 tctctaattt ttttattttt taacatgaaa gaagaagcaa aaaaagcctt atatagtcta
     1081 aaagaaatat tagaaaaata tcctatacat gaaaatttgg atgtaagtca aatgagattt
     1141 tttgataagg tagagtctaa aaatttatat acttttaata tatatacaaa cttgttaatt
     1201 gtacttaatg atgatagtta taatgagctt agagaagaac caatttttaa ggatgtaatt
     1261 gaaaagattt tagatatgga aaaaatgttg aaaaataaag aataagtaaa aatttaatat
     1321 aggacttaaa gattaatcaa attaaaagaa agtgtttcaa aataaaaatt tgaatcacct
     1381 tttttgtctg tcttaaacag ttatttagaa aaatagaggt ttcaaaatag aaatctgaat
     1441 tatctgtatt attacacaaa aatagaaacc taaatttata aaaatagaat aaagtttgtt
     1501 atataaaatg taattttgtc tataaaatct cacctgtttt ctaactttaa gcgtgaaaaa
     1561 gagaatgtat catgaatttt ttagttcatg atgcatcctt ctttgtatga aatccaatat
     1621 atctttatta tttaaacaat ggaaaagagt cttatttcta ttttgagaca tctcttttat
     1681 ttaattatga agttttatac agttcaataa ataggaatta tatttagaaa ctcatgacat
     1741 tatttcttag atagtgatta aatttatcat attccatttt agataacttt tctgggtaag
     1801 acattacaat caggttatgc ccattaaatt tttcatggta tggtggatga atgtgttcat
     1861 aattatttgt cttatatttt actctttcat agaacgcctt gcgataaatt gaaatctcat
     1921 caattggaag gtcaatttct aaaataatag tttttccttt cttatttaaa agctctaacg
     1981 ctctttgtcc atatctatta ttgcgcattt ctggaaaaat gcagaaatgt tctacataga
     2041 taaagtcatc tgtttcccaa cataaaatta tgccgacaaa ctggttttta ttataaatta
     2101 aattgaattg atattcttta tttttcaaaa tttcttcttg caaagaagat tttctttgtt
     2161 catgaaatgg gaaactaatt ttatataatt ccatagcctt gtaatataat ttattttcac
     2221 tggttcttaa ccgttcaaat ttcactttac tcctcctaat cacgaaaatt ccaatttatt
     2281 tatgagttca ttttaccata gtcagtattt gcatacaatg tgttttaagt tgagtgtcat
     2341 ttctttcttt tatttatgtt tatatctcat agactaagag ataattactt aagacataac
     2401 taaaaactat tatattaagc tttgataatc taaaaacagt atatttatgg acttttctag
     2461 gtctaaaagt aatcttaata attttagaag ttacaaaaaa gttacataaa tttggaaagc
     2521 tataatatac atatataata taaggttaaa actgtaatga ataatattaa tttaaaattt
     2581 gaacatggaa ggagattatt atggagaaaa gagaaaagag gataataata tcatctttat
     2641 taagtgtatc aattttaatg ggtttggtaa gtatatattc tatattaaat aaagaagaca
     2701 taattttaac tgttaagggt caagagcaaa aagtatcttc ctttaagaaa acagttgagg
     2761 aacttttgga tgaacagggt gtaaagtata attctgaaga taagattaat ccaagtttag
     2821 atacagaact aaaagatgat atgaaaataa aagttgttaa ggtaactaaa agtaaaaaag
     2881 aagagattga aaaaattcca tttgatacaa agcatgtaaa tgatagtaat ttgttaaaag
     2941 gaaaatctaa agtttatcaa gaaggtcaag aaggagaaaa aaaactagtc tataatttaa
     3001 cttaccatga tggaaagtta gtcaaaaaag tcttatcaaa agaagtaata tctaaggagc
     3061 caactacaaa aattataaaa tatggaacta aagaaaaagt actaatagca tcaagaggag
     3121 caaatataag aggaggcaaa catatgaaag tggttgccac tgcatatgca ggagatacaa
     3181 taacatctac tggtacaact ccaagatggg gtgttattgc agttgaccca cgtgtaatac
     3241 catatggaac aaaagtatat atacctaaac tgggtatgac ttttgtagca gaagattgtg
     3301 gaggtgcaat taaaggtaat agaatagaca tttttatgaa cagtgaagga aaagcttcaa
     3361 attggggaag gaaaagtata gatatatatc tacattaaat tagtgaatag aataacttta
     3421 cttgaaggac aaaaaagtat aaaatatgaa ttaactaaac tgttagatta ttcttaaaac
     3481 ataaaaatat atctaagggg ggataagatg aaaatagtct gcttaggaga cagtttaacg
     3541 tatggatttg gggtatcacg aagtaattca tggacaaaca ttgtaaataa agagactcga
     3601 ttagaaatag taaataaggg tataaatggt gatacaacaa gtggtatgtt agctaggttt
     3661 aatgaagatg ttgtaaaaaa ctctcctgat atagttttta taatgggtgg caccaatgat
     3721 tttattgctg gtgcgggaaa taaaattatt aattctaata taatggctat ggtgcatcag
     3781 tcatattcaa aaaatattat acctataata ggtataccat taaaaccaga tattccaaat
     3841 gttagggaag attggagttg ttttactgat tttaatatag tatctcaaaa cttagaatca
     3901 tatagttatt ggataaaaaa attttgtatg acatttaata ctaatttcgt agatttttat
     3961 tcagaatata ataaaaatat ggaactagaa ggatataaaa aattatattt tgatgggtta
     4021 catccaacca aagagggtca tagaataatg gcatacattt ttattaattc aattaataag
     4081 tatatagaag agttatagat tacattaaaa tgcttgttta tgcgtcagtt gcaataaata
     4141 catacagtag agaggggttt attatggatt atactaaaac ttatgaagag tggataaagg
     4201 gttcatattt tgatgaagat acaaagctag agttggagaa tataaaaaat aatgaaaaag
     4261 aaatagagga tagattctat aaggatttag aatttgggac tgcgggtctt agaggaataa
     4321 ttgaagcagg aacgaataga ataaataagt acacagttag aagagctact tttggattgg
     4381 caaactatat attagaaaac acaacaaaag aagaaacaag tagaggagta gtaatagctc
     4441 atgataatag acacaagtct agacaattct gtatagaatc tgcaaatact ttggcagcat
     4501 gtggtataaa agcatatata tttgatagtt tgagaactac tccagaatta tcttttgctg
     4561 ttagaagctt aaatgcaatc gctggtatag tcataacagc aagccataat cctccagaat
     4621 ataatggata taaggtatac tgggaagatg gagcacaagt tatgccagag attgcaaatg
     4681 caataacaga gaaagtaaat agtatacatg attatagtac aataccaacc ttaacggaag
     4741 ctaataaaaa tttggtagtt ttgttggacg aatcacaaga tactaaattt atagaagcag
     4801 taaaaagcca aattataaga aaagatttag ttaaaaatgt aggtaaaagc tttaagatag
     4861 tttatacacc tctttgtgga acagggaatg ttccaataag aagagcattg aaagaagttg
     4921 gttttgaaaa tataattgta gttccagaag aagaaaatcc agacccaaat tttgcagggc
     4981 ttgattatcc aaatccagaa gagaaaaaag ctttaaatag aggtatatta ctcgcaaaag
     5041 aaaaaggagc agaccttgta atagcaactg acccagattg tgatagagta ggtgtagcag
     5101 taaaaactac tacaggtgag tatgctttac ttacaggaaa tcaaattggt ggtatgctga
     5161 cacattatat aatagaagga ttaaaagaaa ataataaatt aaaagaaaat cctacaataa
     5221 taaaaaccat agttacatca gaatttggag cagatatagc taaagcaaat aatgttgatg
     5281 tattaaatgt tcttacaggt tttaaattta ttggtgaaaa aataaaatta tttgaacaaa
     5341 ataaaaatag aagttatgta tttggatatg aagaaagtta tggttactta gttgggactc
     5401 atgctagaga taaagatggt gtagtttctt cattgttaat atctgaaatg gcagcatttt
     5461 actattcaaa aggtatgagt ttatatgatg gtttaataga actttataaa aagtatggat
     5521 ttttcaaaga acagactata tctttgactt taaaaggtat agaaggtgta gaaaagataa
     5581 aagaaataat atcttatttt aga
//
LOCUS       DAKBNN010000225         5545 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000225 DAKBNN010000000
VERSION     DAKBNN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.231"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            412..1938
                     /locus_tag="OUB02_003417"
     CDS             412..1938
                     /locus_tag="OUB02_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCT8887144.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VKQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1966..2523
                     /locus_tag="OUB02_003418"
     CDS             1966..2523
                     /locus_tag="OUB02_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887145.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            3045..4088
                     /locus_tag="OUB02_003419"
     CDS             3045..4088
                     /locus_tag="OUB02_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887146.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VEGEKIDLNKFEGLDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4199..5341
                     /locus_tag="OUB02_003420"
     CDS             4199..5341
                     /locus_tag="OUB02_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCT8887147.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYIFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2357 a          459 c          954 g         1775 t
ORIGIN      
        1 ctccttttct atttacaaaa gaatataagt aagaattaca attaatttat ataaaataaa
       61 tttttaagta aatattttta tagagagatt gctatattac atatcagaaa aatattaaac
      121 taactaaatc aataaaaaag ttggtggcta acagtttata attgttagca caacatcatc
      181 ttatgtattg aaatctaaaa tatcctcatt tttacttagc ataaatacta ttaattaaga
      241 aaataatata gaaattataa atatatataa gtttagtctg gtgtataaaa caagaaaact
      301 aagtgatttt taatttagct ttttattaag cttatttttt atactattta taataaataa
      361 actccatgat gggaaaacta ttactattta atatgtaaag gagtatttta aatgaatgaa
      421 tcatattggt ttttaaactc aagtccaaaa gaatataaca aattaggtga aaatataaaa
      481 acagattgtc ttatagtagg tggtggaata actggattga caactgcata tttattagca
      541 aaagaaggta aaaaagtagt gcttgttgag gctgataaaa taggatatgg aacttctgga
      601 aggaatactg gtaaggtaac ttgtcagcat gatatatttt actctaagat agagaaaaaa
      661 tatggattag ataaagcaaa atcatattat aatgcaaata atgaagcttt aaatttagtt
      721 aaacaaataa tagaagaaaa caatataaaa tgtgatttta aaagggaaac ttcttttgta
      781 tttacagaaa aagaggatac tataaagaat attaaagatg aatatcgtac atgtaaaaaa
      841 ataggaataa attgtgagta tcatgaaact atagaaaata ttccattaga tataaagggg
      901 gctattagtt ttactaatca aggtcaattt aacccaaaga agtatattga tggattggca
      961 aaagcagcag taaatttagg gctaaaaata tatgaaaata caccagtagt agaccttgaa
     1021 aaaggaaaaa tatgtagggt taaaacaaga gaagataata taatagaagc tgagaatgtt
     1081 ataatttcat ctcatagtcc atggtatgat ggattgaatt tttattttgc aaaagaatat
     1141 gcagagcgtg cttatttaat ggttgcagtt ttggaaaata aattagcaga tggtatgttt
     1201 ataagtatag atgacccatc aataactttt agacaatata atgatggaag tgaaaattta
     1261 ctgatttttg gaggaggaga ccataaagtt ggtcagggag gaactgagaa agaaatattt
     1321 gatgatttag agcattatgg taaagaagtc tttaaggtaa aagattttaa ggggaaatgg
     1381 tctgctcaag ataatatgag ttttgacaat gttccttata ttggttatat caacaaaaga
     1441 gaagacaata tttatgtagc tacagggttc tctaaatggg gaattactaa tggaactgct
     1501 gctggaataa taataaaaga tttaataata aataataatt ctgattataa agatactttt
     1561 aatccatcca gactaggtag ctacttttca aaagatttta taaaggaaaa tgcaaacgta
     1621 gcaataaatt atgtaagtgg aaaattaaaa ataggtagtg gagatatgcc taaaaataat
     1681 ggagaaggaa agattgttaa tattgatgga aaaagatatg gtgtatataa ggatgataat
     1741 ggagattttt atattgtaga cactacatgt actcatttag gatgtgaact taattttaac
     1801 agtgaagaaa aaacatggga ctgtccatgt catggttcta gatttgacta taagggaaat
     1861 atacttgaag gaccagctct taagccatta aaactttatg gacatggtga taatgatgtt
     1921 aatcctaagt tattataaaa ttatttttag gaaggtaaag tgtatatgaa taaactttta
     1981 aagccaataa ctatactatt gtatatagtt tatattttat ctgccgtatt ttttgtagca
     2041 aacaaagaat atggaagtct aggtctagta gcaattagtt tagttggtac tttcattttg
     2101 agttttataa ataagcaaac tgaaagatta ttgagtaaag aattatatat aatgcttgct
     2161 ttatttataa tgttttcatc attgttaggt acttgtttta atttttatga tataaattat
     2221 tatgatgatt ttttgcattt atggtcaggt ataatatctt gtattgtggc tttttctata
     2281 attaaatact tttatacaca aagagagata agtaaaatga gtaaagtgtt tttagttata
     2341 tttttattta tgtttagtat gggagtagcg agtttatggg aaataggtga atttttaatg
     2401 gatacttttg ttgggactac tacacaggcc ggagggttag aagatactat gattgatatg
     2461 gtagatgctt taataggtac aattataact attcctttta tagtaaaaaa atctaatata
     2521 tagttttaaa ttttttatat taattataat ggatttaatt aatcatgttg agtttcaagg
     2581 tctatatgat taattaaatc catttttgtt atttataatt attattgaat atatttttac
     2641 atataagata taaggtgata gatagaaatt gttggattat gtaaataatt aatatataat
     2701 accattatgg gcaataaata caaatcaaat gataatatag tctatttatg taaatgttaa
     2761 attgtatcct aagtataata gtaagttttt tgatagtggt gtagaaagta aaaatctact
     2821 agcctaaatt gatgtatgga ttagtttagg aacagtaatt gcatatctta agtttgatgt
     2881 catgttgggc tactagaaat aggtcatctt tttcacttga atatcttttc tttaaacatg
     2941 gggagtatca tatcaaaaac atatgaaaat taagtgattt atgagaacag taatatttta
     3001 tatgatagta tagaatagta ataaagtatt aggaggaaca ttgagtgtta aaaaagtggt
     3061 ttggtattgt gaaaaaaaga caaaaaagtg agtcagtaaa agaagaaggt gaagtaatat
     3121 taaaaaacga aaaaatatta tctgaagaaa agttgataga tgaagaagga gttgtagtta
     3181 atattgataa tgaaatatta actaaagtag aagtagtaaa tgatgacaat gaaatagaag
     3241 aaaaaataat agaagaagat tggttaatta gtgaaaatac cataaaatta gatgataaag
     3301 aagcaattat taatgataaa aacatagaat tatgtaaaaa agaagttcaa gttgaaggtg
     3361 aaaaaataga tttaaataag tttgaaggac ttgaccaaaa tgaaaattat aatctagaaa
     3421 aaaatgttat tgaagaaaaa gaagtaagtg aatgtttgac agaagaagat ttagagtaca
     3481 taaaagaaat taaaataaaa agaggaaaaa gtataaaagc tataaacttg tatactaaag
     3541 aagagtgggt ttttgacact catatacagt gtagtaaaaa actcaaagtt ccattagggt
     3601 acataagaga aaatttaaaa tatggatata tggattactt tggagatgca ataaattatt
     3661 taagtgaagt attaaatata gatgaatact gcaaaagtga atggagctat ctcgataata
     3721 gcaaatctcc atctgagata tttaatattc taaacaataa aatatttagt ataaggcttt
     3781 ctaatgaaaa aagaaatgaa attttgacaa atgataaaat tgaagcatta aaaatgaatt
     3841 atagatttga atgtattgat gaagaatatg atgaatattt taaaaaatat aagtctataa
     3901 tcaaaagagg tggaaagaaa aaagttgaat tagtaaataa aaaaggtgac attttagaaa
     3961 tatttaagtc tttagaagaa tgtgccattt atttgcaaaa ggaaaagaat gaagttatac
     4021 aaatgttaaa atatggagat acaaaagtag gaagaaactt tataaggtat agtttgagaa
     4081 gtatttaatt tcattttaaa ttcataagag tttcatttct gattcacaca tttaatatta
     4141 ttttaatgta aaataataaa ggtgaagtat tgtacaatta aaattaagga gggtattaat
     4201 gtctgaagaa aaaaatccaa aagaaaatac attagatgaa tctaaaaatt cagataatgt
     4261 aaattctaaa aaagatgatg tgtcagatga aaaaaatact gataacaaag ttaaaaaaag
     4321 ggaaataaag ctaaacaaca aaacacttaa agctattgta gcaattgttg cagtaggagc
     4381 tatatgttta ggtggaggct atatttttgg caaagaagct ggaagaaaat tgcctgctac
     4441 ttcaaaacac tataatagca gtgaggtagt agctactatt ggggataaaa aaataacagg
     4501 tgaaatgtta agaaagagaa tggagccatt attttattta aatgcaaaaa ctactatgac
     4561 tgatgacgaa atacgttcat atgaatcaaa tatgattgaa actatgatta catctgatat
     4621 attatcctct gaagcagcaa aagagaaaat aagtataact aatgaccaac tagatgctca
     4681 gtataataat ttaatgagta gtatagaatc agcaatggga atgactaaag aagagttttt
     4741 aaaacagttt gatttgactg aaaaatatat aaaagatgat atgaaaaaag agctagtagc
     4801 atcaaaatat ttaaatgaaa aatcaaaagt tactgataaa gaagcagaga actattacaa
     4861 taaaaacaag agcaattatt tacaagttag agcttcacat attttaataa aaactgttga
     4921 tgataaaggt aagcaagtta gtagtagcaa aaaagctgaa cttaagaaag aagctgaaga
     4981 aatacttaaa aaagcccaag ctggggaaga ttttgctact ctagctaaaa aatactctga
     5041 agatagtagt gcagaatcag gtggagattt aggtttcttt ggtaaaggtc agatggtgga
     5101 atcttttgaa aaagcagcct ttgcattgaa gaagggagag gtatctaaca aacttgtaga
     5161 aagtgattat ggatatcata taattaagaa aactgatgaa aaatatcagc catttgaaga
     5221 ggtaaaatca gatttagtaa gcagtttaac atcagaaaaa caaaacctac ttatacaaaa
     5281 tctaaaagaa aaatataatg ttaaaattac agataaatat caaatgaaag ctaaagaata
     5341 gataaagtca atttattaaa aaaaagaaaa attaactgta tggataaaag ctatttttac
     5401 tctcaatata gagagtataa aatagctttt attattttgc aataaaatct atttatatat
     5461 atgaagtgac aaagaaataa catgcaaaaa gacattaaaa agaaatattt ttaatttgaa
     5521 ttttttcaat aaaaatttac aaaaa
//
LOCUS       DAKBNN010000226         5526 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000226 DAKBNN010000000
VERSION     DAKBNN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.232"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(100..783)
                     /locus_tag="OUB02_003421"
     CDS             complement(100..783)
                     /locus_tag="OUB02_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCT8887148.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1003..1593)
                     /gene="zapA"
                     /locus_tag="OUB02_003422"
     CDS             complement(1003..1593)
                     /gene="zapA"
                     /locus_tag="OUB02_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCT8887149.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1618..4011)
                     /locus_tag="OUB02_003423"
     CDS             complement(1618..4011)
                     /locus_tag="OUB02_003423"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCT8887150.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4028..5047)
                     /gene="pheS"
                     /locus_tag="OUB02_003424"
     CDS             complement(4028..5047)
                     /gene="pheS"
                     /locus_tag="OUB02_003424"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCT8887151.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5097..5331)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1686 a         1076 c          598 g         2166 t
ORIGIN      
        1 gcttactata ttttaaattc tttaattaat ctatacaata tttgctatat ataaagtata
       61 tcaaataaca gcttcatttt tttgtaaatt tacagaaagt taattttcat taaatgaatc
      121 atctttattg ataaacataa ttccaaagac cactgccaaa ataaaaaaac atattagtat
      181 tgatttttcc acgaaaattt ttgtaaaaaa agttcctaaa atagctccag atataaagaa
      241 tataataata gcataatact gtaaactctt ttttatcata aaaatattct tagtatgtat
      301 ataatgaaat aaagtttctg tagcacttct taaatttcca gtacacattg tcgttgcaaa
      361 actattccca tttacttttc taaaactctc tacttgcatt gaacaaataa aagaaatagc
      421 aacatttaca aacatattaa atcttcctct aggaataaat gaaacaataa gtattgtaat
      481 catttcaatt ataatgataa cctgacgcca atgaatcttt gtcttattat ttgtaaaata
      541 ctttcttatc ttctcggcta taaatacacc taacataaat gccaaaatag gaatcaaata
      601 ataaaatgat tcctttacat taccttctgc tatattaagc ccaaacaaaa ctatatttcc
      661 agtttgtgca ttagcaaaga ctttacctct taccacataa gtataaatat ccaaaaatcc
      721 tcctgttatt gccaacaaag aacataaaaa gaatgattca gacatctgac tcttgcttgt
      781 catatataaa cctctcgctt tctaatgata gatacaaaat caaaatcatt ttagattcta
      841 tttttcattg atataaaaaa attatttata tttaaatatt gtctatattt atacatcatt
      901 tatatttatt tatatttaaa cattatctat atctatatag catttatatt tatacattaa
      961 aagaagctgt ctcaaaaaac aagagacagc aaatctttta gtttattcca cattttttgc
     1021 atcattattt aaccctgtac attttaactg taaatcataa gctttgtttt gaaactcaga
     1081 agctaagttt tcagctatag tagctctttc atttgcttct tcaagttgag tacttaactc
     1141 ctcaatcttg tttttataag catctaactc tgctctagaa ccttctgtcg tattactaag
     1201 ttcgaatatt tcttgctttt tattttctat aatctcaatt aactctttca tcttagtttc
     1261 agcttcacga ttttctgctt ctttttctgc tatcgtaagc tttaaagatt tgatttctaa
     1321 ttgaagctct tcattagatg ctccaacctt cttactcaat tcttcattcg ctttaatcaa
     1381 cttctcattc tcatctgaac attcaaataa aaggtctgat atatttaatg cagttaatat
     1441 cgcagcaact gataagctca atttcgggtt ttgacgtgtt attttatcca tttctttgtc
     1501 tacaaaatca gcaatactaa tcataaactt ctctgatttg tctccaacca ttggatattc
     1561 agcgccatgg attttaacca ttactttgtt catcctaacc tcccccgaca ttagttgtta
     1621 atttgatctt aagttagcat ttaatttttc gcttaattca cttaatatct tatcatgcac
     1681 cttagctaca tcttcatctg ttagagtttt atctttactt ctataagtta ttgaatatgc
     1741 aattgacttg tatccagctt ctatttgaga acctttgtaa acatcaaata acttgtagct
     1801 ttctactaat ccttgaccat tttcctttat tatatcttct atttgtttta caaatacatc
     1861 atctttaaca ataagagcta tatctcttga tgtagatgga tattttggta atggcacata
     1921 attctttgtt ctatcagaat tatcaaatac aaagtcaata tctatttcag cgacataaac
     1981 tctttgacct agattgtagt tttctataac atctggatgc aattcaccta atgtacctac
     2041 atatttatta ttatacacta ttttagcaca tctaccaggg tggaaggtag tattattatc
     2101 ttgaggttca atttcataac ctttaaatcc gacatttaca agtatagttt ctatagttcc
     2161 ttttagtgca aagaagtcaa catctttacc atacatacct acacacattc tattttcttc
     2221 cttaggtaat tctgaatctt gtgggatgaa tatatgtcca cactcaaatg cagatacttc
     2281 ttctatttta tgagatacat tagttgataa aacatctaac atatttggta ttaaagtagt
     2341 tctcattaca gaagtttctt ctcctaatgg gttaagtaat ttaacaaaat ttctctttgc
     2401 attaccttct ggtactctta tcttatctac tcccttaggg cttacaaatg aatatgttaa
     2461 tatttcattt aatcctacag cagttgaatt atcttttaaa gcatccatga atttttgttt
     2521 tgaagtttta attcctgctg tcgtatttcc ttctaattgt acagaaggta tattctcaaa
     2581 tccataaatt ctagctattt cttcccatac atcagcttct tgttccatat ctgttctaaa
     2641 gcttggtaca tctatttcta atttatcatt agctaccaaa ttacatttaa actctaatga
     2701 ttctaaaatt cctacaaact gctccattgg tacatctaca cctaataggt ggttaattct
     2761 ttgaggattt actaccaatt ttttaggttc tggtttattt ggatatacat ctacaacacc
     2821 ttttaaaact tttcctgctc ctaacatttc aacaagttgt gcagctctat ttgctgctat
     2881 ctctgcaaga ttagggtcta agtctttttc atttcttgaa gatgcttctg acctaatacc
     2941 aactttttta gctgtcattc ttatgttttc tggtttgaaa ttggcacttt caaaaagtac
     3001 agtcttcgta ttagaagtta tttctgagtt agcaccaccc attacaccag ctaaaccaag
     3061 tgatttttct ccatttgtta taactagcat atctttgtct aatgttctct caacatcatc
     3121 taatgttaca agtttctctc catctttagc atttcttact actattcttc cagtctctac
     3181 ttgatttata tcaaaagcat gaagtggttg accaagctct aacattacga agtttgttat
     3241 atcgactatg ttacttatag gtcttactcc tgcttctgta agtcttcttt gcatccaata
     3301 tggagaaggt tctattttta catctgtaac cattctagca acatatcttc tacataaatc
     3361 tggattatct atctcaactt tgaaatctat ctcttcgtca ctttctttta cttcgatttc
     3421 aggatatttt acttttgttc ctatagttgc agctgcttct ctagctatac ccatcatgca
     3481 tttacaatca ggtctgtttg aagttaattc aaaatctatt aaagcatctt ttaatcctaa
     3541 aacatctttt atatcttttc ctaattcata agaatcttcg tggtctaaaa tatatatacc
     3601 acctctttta tactcctcaa tgtaatgttc atctatacct agttcagctg ctgaacacat
     3661 cataccatct gataattcac ctctgaaatc agtctgtttg atttcaactc ctccaggtaa
     3721 cttagaacca tttacagcta ctggaatata atctccttct gatatatttg tagctcctgt
     3781 aactatttga acaactttat ctccaatatc tactttggtt acaaccaact tatcagcatt
     3841 tggatgttgt tttatttcta aaatctttcc aaccaatata ttttctattt cttcaccata
     3901 ataatctatt gtttcaactt tagttcctgt cattgtcatt ttatcagcga actcttttac
     3961 atccatgtct atatcaacat agtctctaag ccattttaaa gatactaaca tctacatacc
     4021 ctcctaatta aaattgattt aagaatctca tatcattttc gaataataat ctaatatcat
     4081 ctatttcgta tttaagcatt gcaagtcttt caaccccaac tccaaatgca aatccactgt
     4141 aaacttctgg gtctattcca caatttctaa gcacatttgg atgaaccata cctgaaccta
     4201 atatttctat ccaaccttca tatttacaca ttgggcaacc tttaccacca catttgaaac
     4261 aagtaacatc aacctctgca cttggttctg taaatggaaa gttgtgaggt ctaaacttag
     4321 ttttgatatc agaaccaaac aatttttcaa cgaagatatc catagttcct ttaaattctg
     4381 ccatagtaac atcttttcca acaacaagcc cttctatctg atggaacatt ggtgagtgtg
     4441 tagcatctgg cgagtctgac ctaaaacatc tacctggtgc aattactttt attggtaact
     4501 cttgacttct catagttctt acttgaactg gagatgtttg agttctaagt aataccccat
     4561 cattgatata gaatgtatcg ctcatatctc ttgatggatg gtctttagga gcgtttaatg
     4621 cgtcaaagtt gttttcaaca gtctcaactt ctggcccttc tgctatagag aatcccattc
     4681 cgataaatat atctgttact tcatctataa tttgagttat tggatgcttc ttcccaacct
     4741 ttaaaacttt tcctggttga gtaacatcta tcacttcctc ttttaatttt ctttcttttt
     4801 caatagcatt tatttcttct tttttggaat ttatgctaag ttcaatgttt tctcttacct
     4861 cattggcaac cttaccaact actggtcttt cttcagcaga taatttaccc atttctttaa
     4921 gtatggcagt tatctcacct ttttttccta agtacttaac tcttaaactt tctacacttt
     4981 ctatgctttg tgcttctttt atttcagcca aagctgcttc acgtaaagca agtaattttt
     5041 cttgcacaca tatcaccctt tcgttttttt gtataaaaaa agcccctcaa tccctgtcta
     5101 gggacgaaag gctatagttt cgcggtacca ccctagtagt ttaacaattt aaaaatcatc
     5161 aaaccacctc taacaaactt aacgcgtttt taacgtcaaa gcctactgta aaatttcagc
     5221 tctgttactc cagagggaac ttctcatgac ctcttagaaa acactcccag cctaggtgtt
     5281 tatctcttta ctaagtttaa tcaaaatact ttcctctttc attgcattta caatattata
     5341 ttgtttattt tagtattata tcatttccat tcaaattatg tgactatttt taatatatat
     5401 ccaatacata attaatttaa ttatttttta tataatatat atctaatgca taattaactc
     5461 aattattttt tatataatat acatctaatg cataattaac tcaattattt tttatataat
     5521 atacat
//
LOCUS       DAKBNN010000227         5518 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000227 DAKBNN010000000
VERSION     DAKBNN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.233"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(42..1544)
                     /locus_tag="OUB02_003425"
     CDS             complement(42..1544)
                     /locus_tag="OUB02_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCT8887152.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(1577..2443)
                     /locus_tag="OUB02_003426"
     CDS             complement(1577..2443)
                     /locus_tag="OUB02_003426"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCT8887153.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(2528..3199)
                     /locus_tag="OUB02_003427"
     CDS             complement(2528..3199)
                     /locus_tag="OUB02_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCT8887154.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(3472..3963)
                     /locus_tag="OUB02_003428"
     CDS             complement(3472..3963)
                     /locus_tag="OUB02_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8887155.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EEKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIII
                     DLE"
     gene            complement(3985..5349)
                     /locus_tag="OUB02_003429"
     CDS             complement(3985..5349)
                     /locus_tag="OUB02_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCT8887156.1"
                     /translation="MGEKKINNMNYSHNNYSKNTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NLAD"
BASE COUNT         1844 a          949 c          558 g         2167 t
ORIGIN      
        1 atttatctaa tcactctata agacaattta tcatttaaat attatttttc ataataaaca
       61 gtagtctcag cttttggtaa agatactttt gctttaactt tagcttctat tgcactaact
      121 attgtaccta ccactacaga agtcgtgatt gttattagag aatatgccca agaagttact
      181 tctggcatac ggtattttaa ataaacaact aaaattgctg atactatgaa tccaacatat
      241 gctcccattt tagtcgcttt tttacaaaat gcaccaagta caaatattcc tgctaaaaca
      301 cctagtacta gccccataaa actattaaac cattcatatg ctgactttat ttctccattt
      361 gctaatacta ttgcaacagc tatagaaagt atacctacac ctaatgatat atattgtgct
      421 atcttagttt gcttttccat acttatatct tttgttatga tagtttgtat atctaaagtc
      481 caacttgtag caactgaatt aagccctgtc gataaagttg attgagaagc tgcatatatc
      541 gctgcaagta gtattcctgt tatacccact ggtaattcat aagctatata tgatgcaaat
      601 acttggtctt gttgaactgt ttgagctaaa tctgggtttt gagtatagta tataaataaa
      661 caagttccaa ttagataaaa tactgttgca agtcccattg ataaaacacc atttccatat
      721 gtcattttat taagttgctt tatatcagtt gttgtagtaa acctttgaac tatatcttga
      781 ctcgatacat atgatgaaaa agtattaaga ccagctccaa taaatattat aaatgcactt
      841 gtagatagta tatttttatc aaacataact tcattacttg ctaagaactt gtgtccagtt
      901 gtaagagtat ccattactgt tccaaaacca ccatttatac ttgctattaa atagaacaat
      961 gtccctgtaa cacctactat taataccata ccttgtataa aatcggtcca caagacagcc
     1021 tttaatccac ctgtatatga ataaattatt gctattacac ccataactac tatcaataca
     1081 tttacaggta ttcctgtaag accagctaga accactgatg gaagatacat tatgatagac
     1141 atacgtccta tttgatatat tataaacatc aaagctccaa gtactctaag acctttactt
     1201 tcgtatctca tttctaagta ttcatatgct gtatctattt ctaacctact atatagaggt
     1261 aagaaaaatt ttattgtcaa aggtattgct ataaacatac ctaattgtgc aaaccacaaa
     1321 atccaagtac caccataaga atttcctgct aatgataaaa atgatattgg acttaataat
     1381 gtggcaaata ttgatactga tgtaacccac cacggaatag ttccatcacc tttgaagaac
     1441 tcttttccct tcatttcctt ttttgaaaat aacaatcctg caaataaaac tgccaataag
     1501 taaaccacta gtattgcaaa atctatccaa gtaaatccta ccatttgact aatcctccca
     1561 tactaaaagt attttattaa aaatactttc tgtatatttc ttttgctctt gatttcattt
     1621 catcagttgc ttctttcata ggttgtctac agtatccagc ctcaacacct tgttcttcaa
     1681 gtagtaattt aattgtttga tataagccat ttcctaatat atctgttatt aaatcattag
     1741 tcacatgttg tacttcaagt gcttctgata ttttttcatt cttagttaat tcgaatattt
     1801 gtctagctct gacaccgttt acattgaatg tagaaccaat tgccccatct acacctaaaa
     1861 ctgtagctgg taacatcatt tcatcaaatc ctgcaaatat taatttattt gggaatgttt
     1921 ttctcattct ctctaataag tagaaatctg ctgctgtaaa cttaacacct attatttttt
     1981 cattttcaaa taattctcca aactggtcta aactcatatc aacaccagtt aaaaatggta
     2041 tagagtatat tattaatcta ttatctacag aatttattat tgtattataa tagtgtttta
     2101 tttcttcaaa atcaaattta tagtaaaaag gtgtaacagc acttattgca tcatatccta
     2161 aatcagttgt aaatttagca agttctacag cttcttttaa attaactgaa ccaacttgag
     2221 ctattaattt tatttcttct ttcacttcat ctttagctat ttcaaatatt cttttctttt
     2281 catcagttga aagcatgaag ttttcccctg tacttcctcc tacataaaga ccatctactt
     2341 tacaaacatc tatattgtgt ctaattattt gtcttaaccc tttttcattt atatttcctt
     2401 ccttatcaaa tgaaactaat aatgctgaat aaattccctt catatctgtt gttctcatat
     2461 ctttacctcc aattttaatt ttcaaatata gttaaatata ataatttttt aatttataaa
     2521 ttaattttta attaatttca attgctttaa caaatttttc tgttattaat tgtggtcttg
     2581 ttattgctga acctactact gatgaatgaa cacccaactc aaagaccttt tttaaatctt
     2641 gaggagtatt tacctttcct tcagctatta caggtatttc taaatcttta actaatcttt
     2701 ccattaatac aaaatctgga ccttctaatg ttttagtata tggagtatat ccagatagtg
     2761 tagttgaaac gcaatctaca ccgtactctt gtgctttgat agcctcatca taatttgaaa
     2821 tatctgccat tactagaaca ccgttttctt ttatatactt tataagttct tttaagtctt
     2881 cattatttgg tctaactcta ttggtagcat ctaatgctat catttcacac cctgttgtta
     2941 aaagttcatc aacttctttt ttagttggcg ttatatatat atctgaatct tcatagtttc
     3001 tctttataat acctataaca ggaagtccag taactttttt tatttcaata atatcttcaa
     3061 ctccttgagc tctaattcca acagctccac cttccatagc agccttagcc attctcccca
     3121 ttataaaagg actatgaaga ggttcatctt ctaaagcttg gcatgatact attaatcttc
     3181 cctttacctt atctagcatt ttttaaatta cctcgtttct gaaatttatt ttcttgattt
     3241 atgtattaat aataacatga aaactttttc tttgcaatat tttttcatat ttttttcttg
     3301 catctatatt tttactgatt tattttatat attagcttgt tttttacatt taaaagaaat
     3361 tttatttcat ttttttggtg tttattttca ttttatataa ctactgttat aagtccattt
     3421 aaataatttc aataaaaaaa cccatgtata ctatacatag gtttcccaat attattctaa
     3481 atctattata atttttcttc cacagcatgg acaaaattca gcctctgtat ctatctcact
     3541 ttcacaataa ccacaaatta taaaatcatc acattcattt tccaattcaa attttttacc
     3601 acattcagca caatatttat catttacatt tccaacataa ccacactcac aagttacatt
     3661 actttctgcc atttccattt cattaatttc catatttttt tcataaatta acttatcaag
     3721 ttctagtaac tcatcacaga atttatcaaa actattatca ttaatagaat cttctctaat
     3781 tttttgatat gtcagtattc ccatttccaa gaatatcttt gctttctctt cttgaatatc
     3841 atcaatctct ctatttaatt ttgatttttc tttatataat tcaactttaa attttccact
     3901 accaaacttt ttcttaacct tagaaaattt atcatctact ttattattag tatttccttc
     3961 cacagaaatt ccctccaatc atatttaatc agctaaattt agcttatatc ctattaaaga
     4021 tatcacaaaa ctatatacaa atgaaatcac aatagcttgt aagactttaa attgcattat
     4081 taaagttgtt ccataattag ttgtattaaa gaaattaata ttactatcta gtataaatgt
     4141 actaaataaa gccaatattc ccatcaaaaa tgcataatat atactaaata ctattatagg
     4201 tcttatacta tctgtattat acttaaatct tagcttgtat ccatttaata ataatattaa
     4261 catagacaat gctcccattg cataaaacat taatttagta tctccaaata aagatgaaga
     4321 acttaaagta aacattgata ctgttgagtt tattatggtt actgaaattc catttgcaaa
     4381 agcccacata taactagcta tttgtgggag tatagtaaat aaatttaatc catttgaata
     4441 actactcata tctagttcat ttaagtaact actatcagat atagttagta ccaatagtat
     4501 tactaatact aaaacataac ctaatatgaa tgttcttata gaatttgaaa aaagagataa
     4561 atacatattt tctttttcat aacttttttt ataagtagtt atgtatgtac atataaaccc
     4621 taatacgaat ccatttaaaa gaacactaaa aaattcataa ctatattcta atgcatatcc
     4681 atattctaac atactatgtg aactagtttt tacattagta aaaatagata aaatagctaa
     4741 aactaatcca taaaaaattc caactcctag agaatttgct aatacagttt ttgaatctct
     4801 acatcttttt ctcataaaaa ttaggtttga tattaataat accaaaatag ggattataag
     4861 taatatcaac aaacctatat tggcatttat aaacccagaa cccatcatgg tagacataga
     4921 tacactaatt tgacccaagt tgagagctag gattatatgt gctggattta ctaaataact
     4981 aatatcatta aattgaatag ttataatagc tttaaatatc aatgcaataa taaacaatat
     5041 aaatatggtt gtaaatgttg ttaaaaatac acctctttta tttgcatgat atgaaagctc
     5101 ttttatttta gatttcaagt ctagttttgt ttcaagtggt cttaatgatg ctatttcata
     5161 aagttcattt ccacaaaatt tacagtaatt atccttagta ttattttctt tattgcaact
     5221 agaacaaatt ttttcttctt tacttttaag agtgatatgg ctatttttta tttttctagt
     5281 tggttcacct gtatttttgg aataattatt atgtgaataa ttcatattat ttattttctt
     5341 ttcacccatt ttttccctcc aagttttact attgtaaatt atcaaatata actatatcat
     5401 ataaaactta attatacctt aattggatat atttatctat tttatttgtt tattgctgtt
     5461 gttttttaac aggtcttgat atttaaatag tttgttaatg cgaactttat tatttata
//
LOCUS       DAKBNN010000228         5451 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000228 DAKBNN010000000
VERSION     DAKBNN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.234"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(210..1394)
                     /locus_tag="OUB02_003430"
     CDS             complement(210..1394)
                     /locus_tag="OUB02_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8887157.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1767..4118
                     /locus_tag="OUB02_003431"
     CDS             1767..4118
                     /locus_tag="OUB02_003431"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HCT8887158.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDNLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4140..4679
                     /gene="nrdG"
                     /locus_tag="OUB02_003432"
     CDS             4140..4679
                     /gene="nrdG"
                     /locus_tag="OUB02_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCT8887159.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2136 a          613 c          867 g         1835 t
ORIGIN      
        1 tttttgttat aataaattta gaatttaact tatatagttt aatttgataa tttgatgaaa
       61 aaaataaaag acttactgat ttttaaatct ataggtcttt tattttttat ctaacattta
      121 aattaagttg ttgaatatat cttatattac ttaccttata tattaattac cattaataca
      181 ttatatctaa ttactattca tctaaattac tatgccatta ttttatatac aaattctttt
      241 aatttatcta atccagataa gaaagtattt tcactgcaag cataggatat acgaatatat
      301 ttatccatac caaatgctat gccaggaaca actgcaacat tatattcttc taaaaattga
      361 ttacaaaatt ctatagaaaa actatcttta tattcaaatt tctcagatac tttagaaaga
      421 tctataaata tatagaatgc gccatttgga ttcacatatc ctacattatc aatagagtct
      481 agttttgatt taattaaatc tcttcttgat ttatatgttt taaccatatt gtctatgtca
      541 attgagcaat ctttcaaggc tccataggct atgtattgtg ctgttagact tgggtgagat
      601 ataagatgtc cttgtataga actcattgct ttagcaatcg ttttattaga tgcagtatat
      661 cctaatctta atccagtcat tgcagctgac ttagaaaaac catttatagt tattgttata
      721 tctttagctt cttcgcttaa tgatgcaatt gatgtaaatt caccagtgta gcatattttt
      781 tcatatattt catctgctaa aatatatatt ttattttgta tgcatacatc tactatttct
      841 attaattcat cttttgtata aacactacca gtaggatttg aaggattgtt tattacaaga
      901 atttttgttt tatctgttat agatttttca agttcttctt ttgttaactt aaatccattt
      961 tcttttttag tatcaataaa aactggaaca gcattaacta acttaatcat ttcaggataa
     1021 ctaacccaat aaggctttgg taataagact tcatctcctt cgtctgttaa agctagtaaa
     1081 gtatttgtta ttgagttttt tgctccactt gatacaacta tttcgtctat tgaatagtta
     1141 cagttatttt cttcaataag ttttttacag atttcctctc taagtatttt taatcctgga
     1201 actaaatcat atttagtgta atctttattt aaagaatcaa taccataaga ttttgcgtta
     1261 ttaggtacat taaaatcggg ttctcctata ctaagattta ttactttaac tccattactt
     1321 tccatttctt taactttaga actaatacct attgtgtagg atggtgttat aaagtttagt
     1381 ctttttgata acataaaatg cctcctttta aattattatt tatattatac gacaaattta
     1441 aagaatttac ataaggaaat ttgtagatat atacattaat atatataata gcctacactt
     1501 tttgcctaaa attagtgtaa aaaaaagaga aaaaaatttt aactttcaaa gttgttagaa
     1561 acgctgctgt tcaaaatatc cacatagtat ttgcaatgat ttgcgcaagt ttgactatta
     1621 tgtacaaatt tggtaatctt aatatagcaa ttattaagat aatcccttta acttagttga
     1681 aggggatttt tttagactta acatactaca tatagtggta tggggattaa taaaatacaa
     1741 tatatagtaa caggaggttt ttttaattgg ttgaattcgt aaaaaaaaga gatggaagag
     1801 taattccatt taatgaagat agaataacaa gagctatatt tttagcagca acgaatgttg
     1861 cagaaagaga aggaatagta cctgattata aattatctga gcaattaact caagaagtaa
     1921 taaagttttt aaatcataaa tactcagaat cagttccaag tgttgaagat atacaagatt
     1981 ctgtagttaa ggttcttata gaaactggcc atgcaaaaac tagtgaagaa tatataatat
     2041 acagaactga aagaagtaga ataagaaatt caaaaacaag gttgatgaag gcaatagagg
     2101 aaattacctt tgaagatgca gaagatgctg atataaaaag agaaaatgca aatattaatg
     2161 gtaatacagc aatgggaact atgttacaat atggaagcac tgtatctaaa gaattttgta
     2221 aaacacatat attaaaacca gagcattctt ttgctcatga taatggagat atacacatac
     2281 atgatatgga ttttttaaat atgggaacat taacttgttg tcaaatagat gttaaaaaac
     2341 tattcaatgg tggattttca acaggacatg gctttttaag agagccacaa gatataataa
     2401 gttatggtgc attagctgca attgctatac aaagcaatca aaacgatcaa catggaggtc
     2461 aaagtatacc atttttcgat tatggattgg ctgaaggtgt atataagaca tttaagaaat
     2521 tctacatagg aaatttagct aaagcattga agttatttaa aggaattgaa aataatgatg
     2581 tgataaagaa catagtatat aatacagaga aagaaacaaa tcaaaaagtt ggtcttaaga
     2641 gagatgaatt gtatctaaac ttagaaaaag agaaattaat acagactttt gatatagatg
     2701 atgaacttgt aaataagatg caaaactttg catttgaaga atcgtataga gaaactgata
     2761 aaaaaacata tcaatctatg gaagcattta tacacaatct aaacacaatg cactctagag
     2821 caggtgcaca ggtaccattt tcaagtgtaa acttcggaac agatacttct gaagaaggaa
     2881 gaatggtaac taagaatctt ctattatctc aagaaagagg tttaggtaat ggagagacac
     2941 caatttttcc aattttaata tttaaggtca aagaaggcat aaatttaaat cctgaagacc
     3001 ctaattatga tttatttaaa ttatcatgca gagtatcagc taaaagacta ttccctaatt
     3061 ttagcttttt agatgcgcct tttaatgcaa aatattataa aaaaggtgaa ccagatacag
     3121 aggctacata tatgggttgt agaacaagag tacttagtaa tgtgtgtggt tcagaaacag
     3181 ttagtggtag aggaaacata tcttttacta cagtaaattt accaagattg ggtataaaac
     3241 atggtattat aaataatgaa aaggctaatt tagatggttt ctttgaagaa ttagatgaga
     3301 agataaactt aattatagaa caattattgg agcgccttga ggttcaagga aataaaaaga
     3361 tgaagaattt tccattctta atggggcaag gtgtatggaa aggttctgat aatttaggac
     3421 cagaagatac actaaaagaa gtaataaaac aaggaacatt gacaattgga tttataggtt
     3481 tagcagagtg ccttattgct cttataggta aacatcatgg agaaagtaaa gaagctcaag
     3541 aattaggatt aaaaatagtg tcacatatga gacataaaat ggatgaagca acggacaaat
     3601 ataaattaaa tttctcctta atgggtactc cagcagaagg tctttcaggt aggtttacta
     3661 aaatagataa aaaagtttat ggagaaatta aaggtatcac agataaagaa tattatacta
     3721 attcattcca tgtaccagtt tattacaata taagtgcata tgataaaatt gagatagaag
     3781 ctccatacca tgaattgact aatgcaggtc atataacata tgtagagtta gatggagatc
     3841 catctgataa tttagaagct tttgaaactg ttataaaagc tatgaaagat ttgggtatag
     3901 gatatggaag tataaatcat cctgtagata gagacccaat atgcggtttt tcaggtgtaa
     3961 taacaagtaa tatatgccct gtatgtggaa gaaatgaaga tgaaagtgac atcaaattcg
     4021 aaagaataag aagaataaca ggatacttag ttggtacagt tgatagattt aataatgcta
     4081 aaaaagcaga agtaagagat agagtaaaac atagatagtt ttatttgggg gaataagtta
     4141 tgaaaataag aatgtcatct actataagtt atgacagcat agttgatgga ccaggattaa
     4201 gaatggtaat atggactcaa ggatgtattc ataattgtaa agagtgccat aatcctcaga
     4261 cacatgattt atgtggagga ttttatatgg atacagaaga aattataaat aaagtcaaat
     4321 cattgaaatt acaaaaaggc attacgctat caggaggaga accattttta caaccagaac
     4381 cattagaaga aatagcaaga gaagctaaaa taaatgggtt agatgtatgg tcatatactg
     4441 gatttacgtt tgaacaactg ttagataaaa aaaatagagc gtattttaaa aatttaaatt
     4501 tactaaagca aattgatatt ttagtggatg gaaaatttat agctgaaaaa aaagatataa
     4561 gcttaaagtt tagagggtca tcaaatcaaa ggataattga tgttcaaaaa agtttgaaat
     4621 ataaaaaagt gtttttggtt gagcaatata tgaaggatga tttatctata gcagaataaa
     4681 atataaataa acaggattaa atatatgcat atatttaatc ctgtttattt tcatataata
     4741 tttcaagctt gtataatgta acaattaaac caaaagactc attgtatatt tgaaattaag
     4801 ttttttatag tttataggtg ttttatgtgg attacgaatg atatagtatc gatacattat
     4861 ttaatagatt cttatgccaa gaaacaatta atttatagaa attcttaatt gttacaatat
     4921 aaatatctag ataagttata agatactgat aaataattta taaagtttat taatctaata
     4981 ttgtgtgtga atattaagtg gtcaagtagt ctaaaaaatg aaaaagattt aaaaaagtgt
     5041 tttaactaaa cacataagtg ttaaattcat gtataagtat aggttagata cataagaaat
     5101 aattatgtaa tatctatctt tgtaaaaaaa tattaaattt tgtaatttgt ggataaatat
     5161 gtttataatg tggataatat ttaatgtaat aagttaaagc aatatgaatc aactgtttat
     5221 aatgtggata atattataaa tgtatgaata attgcagtat ttaaaaacaa aaaaagttaa
     5281 aaaagttgtt gactctaaaa gaaaaagttg gtatagtaat acttgtcctc aagaaaaggg
     5341 caaacaaata aagcacaaag aactttgaaa attaaacagt aggttaattt ataaaacaag
     5401 aaacaaacca taaagccaga tattttgata acaatagtat ctgagcctga t
//
LOCUS       DAKBNN010000229         5410 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000229 DAKBNN010000000
VERSION     DAKBNN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.235"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            252..731
                     /locus_tag="OUB02_003433"
     CDS             252..731
                     /locus_tag="OUB02_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCT8887160.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAINNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            728..1507
                     /locus_tag="OUB02_003434"
     CDS             728..1507
                     /locus_tag="OUB02_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887161.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANDVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1757..2257)
                     /locus_tag="OUB02_003435"
     CDS             complement(1757..2257)
                     /locus_tag="OUB02_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887162.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(2869..3576)
                     /locus_tag="OUB02_003436"
     CDS             complement(2869..3576)
                     /locus_tag="OUB02_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT8887163.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(4380..5249)
                     /locus_tag="OUB02_003437"
     CDS             complement(4380..5249)
                     /locus_tag="OUB02_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCT8887164.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITSEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT         2009 a          795 c          561 g         2045 t
ORIGIN      
        1 ataaacactt actataaaac taaatatata aatggcttct aaagtttaat atatctacat
       61 ggctttatat atgaggggaa aatactagaa aaaaatatat tatagaatat ttgtatagaa
      121 taggcaaaac tttcaaactt aaatttatca aataatcata aaaaaagtga accatctata
      181 gtaaaatttt atctaataag tgaaaggcaa aaaagtagcg ctacgatttg catagaaggg
      241 aaggaactta gttggataaa actacattta caaacaatat acttgaatca gaacagactt
      301 tgtatcgagt atctaagtct attctaggaa acgaccaaga ctgtgaagat gccatcaaca
      361 atgctatttt aaaagcatat gaaaagttgg attctcttaa ggaagaacaa tactttaaaa
      421 catggctgat tagaatagtc ataaatgaat gtaattcatt aagacgcaaa cgattaaaat
      481 ctctatcatt tgaagatgtc ttcaaaaaca aaaaaattga tgaaaaagat gactatagcg
      541 atttgtatac tgctattcaa agtctaccta aaaaaattaa aattccaata gtcctttact
      601 atattgaagg ttattcagta gatgaagtta aggaaatatt agatatacct caaggaactg
      661 taaaaagtag attatcaaga ggaagaagat tattaaaaac taaattagaa aacacggagg
      721 taatcatatg aataaaaata tattaaataa ttcgtttcca gaagttccag aaagttttca
      781 taatagttta agcaaaacac taaatagttt accagaaaga gaggaaaatt ataaaatgat
      841 taataataaa acacacaaat taccatttag aaaaggctta gttgcaactt tagccattac
      901 tttgatattg agcacaacag cccttgcagt aggacatata tatagtttag ttggaaaatc
      961 aagtactaag gctacctata catctatccc aactacagaa caagtaaaaa aagactttaa
     1021 ctttacccct aatatagttt ctgaatttag taatggatat aaatttaaag gagcatatac
     1081 tgtaaataaa aaagcatttg acaaagaaaa taatgtttta ggaaaatcaa aagcacttag
     1141 ttttgaatat aaaaatggca atgatgaaat atctcttgac acagcaaatg atgtacttgg
     1201 gaagagttct gacaatctaa aagttgtaga tacttacaaa gatattaata tttcttatga
     1261 agcattcgca caaaagtttg taccagaagg ttataaaatg acagaacagg ataaaattga
     1321 tgaaaaatca ggcaaataca catttagtgt ggggccaaat aatgaatcag acaaagttga
     1381 agttaataaa tttaagtttt tgacatggaa acaagatgga gtttactatt cttttacagt
     1441 tcaagactca aatttatcta tgaatgactt agttaaaatg gctcatgaag taatagatgc
     1501 aaaataaaca taagtaataa taagttgttt atgagtttat tatttatgac tttaattttt
     1561 ttattagtaa acatggttgt atcttccatc tttatatttt ctttaaattt aatatataaa
     1621 attttagata tttagctacc ttcaaaagaa ccattattaa aaagccctta aaaatatttc
     1681 ttttatatgg aattttaagg gctttaactt tattttatac aagtgtatat ggaattttaa
     1741 agactttaac tttattttat ataagtgtaa gctccatttg aattacttct tcttttacag
     1801 gtctatcatt ccatgtaaaa ccactagata ttgtatttac ctctacaaac cctaaagatt
     1861 tataaacatg aatagctcta ctattacttt taaatacatc aagctcaatt ttttcatacc
     1921 catattcttt tgataaatca ataatttgtt ttaaaagact acttccgata cctttttccc
     1981 tatactcttt aagaataccc atgcctagat aaccaacttt ttcagtttta ggtaaaacgt
     2041 cacaccaacc aatacagtta tctgattcta aatcaattat aaacagttgt ggtaagtttt
     2101 tatttataat attttttata aattcaactg tttcttcaaa aggaaaggct tcatccatag
     2161 ctagatattt cctctctttt gctattttat caaaagtttg ccaatacgaa taaatatact
     2221 tttcttctgc ctttataaat tttatgcctt ctatcaactt aacatttccc ctttttaatt
     2281 tttgattgta gttaacttat tttatatgat tctattttat aatgctatat aaattactat
     2341 atattagctt aaaatatctt tgtatatttc tataaaatta aaaccaatat atctatctct
     2401 tgtatctcta ttaaaataat ttttaatagt ataataaata tagctcttta atcttttgag
     2461 tatatatact taatcatttt gaaatttgat ttaaagtata ataaaatata taaaaaaata
     2521 tataaaatgg tactataaaa tttttagtct tcatcaatac cttttttgct ttcattgtta
     2581 aagctatttt ttctattagt ttatttctta taaaacaaac atttagtatt aaaatatata
     2641 tcacacaata gtatttgcaa tttaacttta aaaaaataaa aatacccaac tgacctaagc
     2701 aactcgattg ggtataaata atctaaaaaa tcatttttgt ataaactctt tatttaactt
     2761 acatttttat ttaccttcta ctttaattca acttatattt ctatgcttct tatactctca
     2821 ttcaacttat atttctactt atcttatact tttataaatt catcacaatt atttagttct
     2881 cgctatcaaa gaaattgtat taaaatactt tccattctct acttccatca tttttttgtc
     2941 atttatagca tatgaattat cacaatttat ccaatccttc caagcttcat catgacaatt
     3001 taaagcttca catttctcta caatcacaga ctccgattca gaccaaagtt tattccacca
     3061 tgtaattgaa tggaagttca tattatcttg ccaaaatgga atgagttcat ctggtataca
     3121 atccacgaaa tcttctttta atccaggcat tgccatagca agtattcctc cctcttttac
     3181 aagaggtgat atatgattgt ctaaaaatcc ttctttgttt ccaaagtagt gataagaatc
     3241 gatacttatc accgcatcaa agaaaccttc tgcataaggt agttcatgtg cctctgcctg
     3301 tattggaaat attttatcat ccaatttaaa ctctttaaat cttttatagt tttcagttgg
     3361 ttctatccat aaatctgttg caaatacagt agcatcaaat tcctttgcta aaaatatcga
     3421 agatataccc ttaccacaac ctaagtctaa aatacgcatt cccttttcta gttttatctt
     3481 acttgttaat tcttccaata tttttataca attaggtccc atcatatttt tcatgagaaa
     3541 gtccttatcg tatttattac tttttgtaaa tatcattcta tctttccttt ccctatcagt
     3601 tattatttat taaattttat acgtttgatt ttttattttt tatatgcttc ttatattgag
     3661 caatctcttt tttattcatt ctttaacaga caattttaat gtgtaatttt atccactctg
     3721 tgatagtaac tttaatgaat aatcttatct actgtgatag ttaactttga tgggtaattt
     3781 tatctactcc acagcaatcg ctttaatgag taatcttatc cactctatga tagttatttt
     3841 agtgagtaat tttatctact ctacagtaat cgctttaatg agtaatctta tccactctat
     3901 aatagtcact ttaatgagta atcataattt ttatatcatt ttagatactt actctaataa
     3961 gaagtcattt tctaatactt aataagtttt aattacttcc actttaacct atatactaat
     4021 taacttattt aatttagtgc atatttgaca ctttaccttt tattatacca tttattatca
     4081 taaatataat aattctattt ttttaaaata aatatttata ataatctaaa ataaatattt
     4141 ataaattcta atcaatccta aataataatc taaaatacat caaaaaataa actaaaaaat
     4201 atcgaaaaat aatttaaaag acatcaaaac taaatcttct aatcagcaat taaaagtcta
     4261 ttttcttagt ttaaaaaata aagatagatt ttttcaaaac actggataaa taggtaaaaa
     4321 aataaacagt tagtaaaata aatttttact aactgtttat ttttattgtt taatatgtcc
     4381 tataaatcct ctaagtaata ttttccttgc aaaaattctg attctaaacc tagattttta
     4441 aaataatcga ccatgcccat acacgcaaat ttttctgtag aataatgagt agcacctata
     4501 acattaatta aattatcttt tgctatttga tgaaactcta atactggttc aaaatgtttt
     4561 aaaggcttag taaacccagt aatatataga tttataccaa gttcagcaac ttctcttgct
     4621 acaaatggat aacttcctcc ccctgctgca atagcaacta aaccatcttt taatacagaa
     4681 tctccataat catacaattt cacatcatga ccaacagatt ttttaattaa ttccttaatc
     4741 tcttctattg attttagtga tgttttacat ataactccta cctcaatttt atcgtcatat
     4801 ttacaaaatg gtttgactat ttctaaaccc atagcttttg caaaactaac agaagttgaa
     4861 tagtcactat agttgtctag tggagaatgt aatacataaa aagaaattct acgatttttc
     4921 atctcttcca tataagatag tggtatgtcg tgaaatggaa aaccttcatc tgatgctatg
     4981 tatcccattg catgatgtga aaaaattaaa atatcttttt catctttatc tataacctgc
     5041 ttaattattt ctttatcagg aaaagtagtt gtgtatactt tattaatatt ttgagcatta
     5101 tctaatacta atcctatgta tttttttcta aattctgaag tgataaaatc attttcaaaa
     5161 ttcataaaac tccaatcatc attaatattt ttaatattaa attctaaatc taatttttta
     5221 taaagttcaa ttgccttaat aataaccact cctttataat aatattgaat tactacatat
     5281 taatatagaa atttcacgat aataaacttt atacgtttaa gtattcctac ttaaaattaa
     5341 aaatattata aatattcaaa tttattaata tattttaata ttatatttat tttttcttta
     5401 ttaatattcc
//
LOCUS       DAKBNN010000230         5401 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000230 DAKBNN010000000
VERSION     DAKBNN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.236"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(260..976)
                     /gene="dapD"
                     /locus_tag="OUB02_003438"
     CDS             complement(260..976)
                     /gene="dapD"
                     /locus_tag="OUB02_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCT8887165.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYDDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1200..1949)
                     /locus_tag="OUB02_003439"
     CDS             complement(1200..1949)
                     /locus_tag="OUB02_003439"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCT8887166.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2016..2903)
                     /locus_tag="OUB02_003440"
     CDS             complement(2016..2903)
                     /locus_tag="OUB02_003440"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCT8887167.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3020..4021)
                     /locus_tag="OUB02_003441"
     CDS             complement(3020..4021)
                     /locus_tag="OUB02_003441"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCT8887168.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4446..5327)
                     /locus_tag="OUB02_003442"
     CDS             complement(4446..5327)
                     /locus_tag="OUB02_003442"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCT8887169.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         1790 a          969 c          653 g         1989 t
ORIGIN      
        1 aaattataaa aagtttaata gtttattaca ttgatatatt gaaaaattga aggaaataaa
       61 aatacttgtc agcaagaaat aaaaatgata taagaatgaa taagagaatt gtaaaaaaat
      121 aaaatgatta taaataaaag ataaaaatat tgtaaaaaag agggtgcctc tggtgaatta
      181 aattcacaca gagacacctt tttaattaat tttttaaatt agtatattat tgtttaatta
      241 atagtatagt actatttaat tagtctaaat ttctaaggtc ttccataagc ttagttttgt
      301 cagccgtttt ttcatctttc atttttataa ctttagcagg agaaccagca acaacagcac
      361 cagcttctac atcagtagtt acaacagcac cagctgcaac aacagcacct ttaccgattc
      421 taacaccttc aagaataact gcattagctc ctattaaaac atcatcttca actataacag
      481 gagtagcaga aggtggctct aaaacaccag caacaacagc accagcacct aagtgaacat
      541 ttttaccaag agttcctcta gctccaataa cagcattcat atctaccata gaaccttcac
      601 caataacagc tcctatattt ataacagctc ccatcattac aacagcattt ttttctatag
      661 taaccatgtc tcttattatt gcacctggct caattcttgc atgttcactt aaagtatttt
      721 ttagaggaat agcagaattt cttctatcat attctacatg agtatctgtt atatcatttc
      781 catgtttatc taatacttcc attatatcat catagtcacc tacaaatata taagaaccat
      841 tatttccaaa taccttaaat ttatcagttg attggaagtg taaagagtta gtatttacat
      901 ataccttaac aggtgttttc tttttaactt cttttatata ccttgctatt tcataagcat
      961 cgtttaaatt taccatttgt taattctcct tgtatttcat tatatttctt ttattatata
     1021 cttatttgtc aattttagta tatacttact tcttattatt tatattttat tttagttagt
     1081 ttttattgta tagacatagt attaaataaa tttattatat atgtttgatt tgtattgcat
     1141 attattatat atatattgta tatgtttaat ttatattgtg tattattata tttatactat
     1201 tatcccaaca tgctgtccat attataaaat cctggttttt gcttaactaa gtatttggca
     1261 gcagctatag aaccacttgc aaagatacca cgagattgag cttgatggct tattgtaaga
     1321 acctcattgt ctccagcaaa tagagcatca tgttcgccaa caatatttcc acctcttata
     1381 gcatgaatac caacttcatt tggttgtctt tttgcactac gaccatatct accataatta
     1441 ttttcaacgt ttggcaaaac ctcttttata gcgttagcaa tcataacagc tgtgccactt
     1501 ggggcatcta cttttctgtt gtgatgtttt tctattattt ctatatcaaa tccttctagt
     1561 aatttagctg catctttaac caattttact aatacattta caccaagaga catattatat
     1621 gattggaata caggaataac tttagccgct tcacgtattt tgttcatttc gtcatcatta
     1681 tatccagtag tagcaattac taaaggtgtt ttagtcttta aaacgtaaga taagatatta
     1741 tctaaattag agtgatgaga aaaatctata actacatcag cttcctcaac tatagtactc
     1801 atatcttcat aaattttaag gtcgccttca tagctatcca gacctctagc tgcaacacaa
     1861 accaattcta attcattatc ttctcttata gtatcggcta aaatcttacc catttttcca
     1921 tcataaccag ttgctataac ttttaacata atttcctcct atgattaagg gctaactttt
     1981 gaccaaactt actttttacc atgcattaat ctttactatg cattaacttt tagcccaaag
     2041 ttaactaatt ctttctttaa tacttctaaa tttgctgggt ccatttctgc tagaggtaat
     2101 cttaaatctc caacattgaa tcccaataaa ttcattgcag tcttaactgg tataggattt
     2161 acttctataa ataaagctgc gattagagca tccataccaa gttgtaattt tctagaacct
     2221 tcaatatcgc cttcaaagaa tttagctacc aaatcgtgag tttcttgagg gcaaacatta
     2281 gcaagtactg atataactcc ttgacctcct aaagataata aaggaagtat agtatcatca
     2341 ttaccagagt agatagcaaa gtcatctgga actaaactag ctatttctgc aacctgtcct
     2401 aggtctccac tagcttcttt tacagcaact atgttatcaa ttttagcaag ttcaactata
     2461 aggctaggac ttatatttac acaagttctt ccagggacat tgtaaagtat aattggaagt
     2521 ttaacactgt tagctattgt ttcaaaatga cgcttaagtc cagctttatt agctttattg
     2581 taataaggtg ttattattaa tagagcatct actcctaatt tttcagcttc ttgacttaag
     2641 ttaactgagt gcatagtatc attagaacca cttccagcta taacaggaac tcttttatta
     2701 actttttcaa caacatactt aatagtagca agttgttctt catcagtcat agtattagct
     2761 tcaccagtag taccacaaga tactatagca tcagtaccat tagctatttg gtattcaact
     2821 aattcaccta atttgtcaaa atcaactttg ttatcctttg taaatggtgt aactagagca
     2881 acagcagaac ctttaaatat catataaaat caatcccctc ttttttattt ttgtgtttta
     2941 tattacagtt acttctaatg cttgtttttg agttactatt aatattttac aaaattatat
     3001 atttatacaa gtttgtatat tatactaaat cgtatgataa agccatttca gctatttgaa
     3061 ttgcgttagt tgcagcacct tttcttatat tatcagcaac aacccaaaga tttacaccat
     3121 tatcaacact aaaatctctt cttattcttc caacaaatac ttcatctcta tcatcaaatt
     3181 ctacagcagt tggatattcg tttttagcag gattatcaac tactacaaca cctggagctt
     3241 cttcaagtgt ttttactaaa tcatctaact caaatggatt gttaaactca acatttatag
     3301 attcactatg accatttttt actggaactc ttacagtagt tgcagttatt tttaaatcgt
     3361 agttatcaag tattttcata gtttcattta ccattttcat ttcttcttta gtgtatccat
     3421 tttcagtgaa tgaatctata tgaggaagac agttgtaaga aatttgatga gggtagaatc
     3481 cagtaggatt accgttcatt cctttctcta aatcttcaac acctttaaca ccagaaccag
     3541 atacagcttg gtaagtagag taaacaactc tcttaatttt gtatttatca tcaagagctt
     3601 taagaggaac catagcttgt atagtagagc agttaggatt agctattata cccttatgat
     3661 tttttatggc ttctggatta acctcaggaa ctactaaagg cacatctgga ttcattctcc
     3721 attgactaga gttatctact actaagattc catttgaagc agctattgga gcatactttt
     3781 cactaatact tccaccagca gagaataatg ctatttgtat gcctctatca gtaaatgaag
     3841 tttcctttaa ttcttctact atatattctt taccacaaaa cattacttta gaaccagctg
     3901 atttagaaga agagaataaa aatagatttt ctattggaaa atttctttct tctaatactt
     3961 ttaaaaatgt tctacctacc attccagtag caccaactat tgctacattt acttttttca
     4021 tataaattca ccccaatttt aaattattaa aacattattt tattttagtt atacaagtaa
     4081 gatatgttaa tatttttata tatccaaaaa ctttaatatt atattaacaa ctttaattat
     4141 atacaaaaaa cgtacaaagg tatactttgt acgtctaatt tatcactcaa agtaaaccct
     4201 gtagctcatc acctacaaaa ataagtgaca gtattacaca tgttatgtgc aatcccagaa
     4261 aatattattg caagaaatat tttcttcggc tgtaaatcct ttttccaatc attaacacct
     4321 gccagctact attggatact cttagttaca gctacctcta ccctaggtta ataggatatt
     4381 aaaatttgat tattctaata ataatacata tttaagaaaa aatcaactta tttatataaa
     4441 tattgctaaa atttaaatcc aaaatttgtc aattcttgtt ttaacacatt aagattagtt
     4501 tcttccattt cagctagagg aagtcttaaa tcaccaacat taaaacctaa aatgttcatg
     4561 gcagttttaa caggaactgg atttacttct ataaataagg cagcaattaa agcatccata
     4621 tcaagttgta atttttttga gccttcaata tcaccttcaa agaatttagt tactaaatca
     4681 tgtgtttctt taggacatat gttagcaagt acagagataa caccacttcc acctaatgat
     4741 aatagtggta gtatagtatc atcattaccg gagtatatag caaagtcttt aggtactaat
     4801 ttagctattt ctgcaacttg agctaagtct ccacttgctt ccttcacagc aactatatta
     4861 tctattttag caagttcagc cacaacagat ggctttatat tcactttagt tcttcctggt
     4921 acgttgtata aaataattgg caacttaacg ctatttgcta ttgtttcaaa gtgaagttta
     4981 agacctttct cattagtctt attatagtaa ggagttatta ttaaaagacc atccacccct
     5041 aatttttcag cttcttgact catatgaaca gacaacatag tattgtttga accagtacct
     5101 gctataacag gtattctctt attaactttt tcaactgtat attttattac agcaaaaatt
     5161 tcagattcac tcatagtagt cgcctcacct gttgttccac aaacaactag tgcatctgtg
     5221 ccattctcaa tgtgatattc gattaattca cctaattttt caaagttgac attattatcc
     5281 tctgtaaatg gggtaactaa agcaacagca gaacctttaa ataacatata atcaaatctc
     5341 cttttttatc taaatttaat tttttaagta caataataat tatagtacaa attaattaat
     5401 t
//
LOCUS       DAKBNN010000231         5280 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000231 DAKBNN010000000
VERSION     DAKBNN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.237"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            179..1042
                     /locus_tag="OUB02_003443"
     CDS             179..1042
                     /locus_tag="OUB02_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCT8887170.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            1121..1933
                     /locus_tag="OUB02_003444"
     CDS             1121..1933
                     /locus_tag="OUB02_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8887171.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKRLYILNSSKPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            2175..2900
                     /locus_tag="OUB02_003445"
     CDS             2175..2900
                     /locus_tag="OUB02_003445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887172.1"
                     /translation="MKDIIEIYDNYNNIGVKNRTITEGKEIELVREYINFRKSEFQPT
                     ENRKLAIFLEAKVDNAYPDIVFVEYSPENYINWNEARAELNKDDLKILYHIYITGGID
                     STGLVKQLGVNWKEAMLAIEKLSDADLIIRNYKKWKIKDEKKLTTYTIEAVEAKLNNW
                     DEVLQQSIINKNFASESYALSIMKSKPKEETLKKFRKFGVGVYLKNENGFKRIKKARS
                     VTMPVSFNSIFFNEWIGRIINLG"
     gene            2913..3755
                     /locus_tag="OUB02_003446"
     CDS             2913..3755
                     /locus_tag="OUB02_003446"
                     /inference="COORDINATES: protein motif:HMM:NF012298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCT8887173.1"
                     /translation="MNDKQIIENFNVREVIKRFPKSGQKQVFLVNHKVYGKVILKLVQ
                     SGDERVKREIEIVTENNFYNVPKVICVGNYDSSEECGIYILEQYIQGMSLREIIKEKS
                     ITLSEELVLAETMLKIIVQMEQQKIVHRDIKPENILRNTEGEWYLIDFGIARALDMSS
                     LTFTKVEIGPHTPGYGAPELFQYNKKDIDSRADIFSLGVVLFEAVTGYHPFIKGDEMN
                     LNEIWYNTVTVMPESVVIEGDYNMQYISLIQTYMQKHVTRRPNTAQKALEWFYNVKDN
                     LLKG"
     gene            3763..4968
                     /locus_tag="OUB02_003447"
     CDS             3763..4968
                     /locus_tag="OUB02_003447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887174.1"
                     /translation="MELFLQFAWGMKQMVLDLSKDWSGATTILSPRDISPNQLERWVK
                     EFKKANVKTLFDPQCYYPKCNHKNLVQYNYWDSSFSTKMQGSFSFEDELIKSILYYNN
                     IAETHEFIIPSNMLTHDEKWFSRWLKNSEQLVNSARKIVRDRKIILTLAIPDSVLLQK
                     EDEVEKIIEATEKLDVDGYYIIAHPPQDKYLVDVPMWLINLTQLCAGLKLQSRKVIMG
                     YGNHQLLCLTATGIDAMATGTYLNVRRFTNKFEEDDSIKRKSIWYYYPAALSEYKMGF
                     LDAAYNNGVLQQMRPAKEMDRGYVDLIFSDVLPSSTAFKEPMAFKHYLNCVKVQMKSL
                     LKQTYKETITANEIMLENAMRRIEYMEKSGVYAQGRSFKDMVDVNMSALQRLDKNRGF
                     QLQQEWNNF"
BASE COUNT         2161 a          508 c          975 g         1636 t
ORIGIN      
        1 taataatagt acagaactcg taaagcattt atatgctcaa atatacggga cattgatttt
       61 tgaaggaacc cgccaagctt cttttatagc tcaaataggc gggacagata aatttaccca
      121 ctaatattaa ttggtgggtt ttattatata aaagtatatc tatgaaaagg tgaaaacaat
      181 ggttgaagta aaagaagaaa aaacatttga tgaacaaata gatattttaa aaagtagagg
      241 attaataata aatgataaag aagatgctaa atttgtatta agtaatgtca attattatag
      301 gtttacagca tatcttctaa gttttaagaa tgatgatggc tcatataaag aaggaactac
      361 gtttgaagaa gtttatgata tatataggtt taataaggaa tttaggatat tattaacaga
      421 tttgttaggg agcatagaaa tagcatttag gacatacatt gcatatacat tagcaattaa
      481 acatggtgct tgtggatatc tagaaaggga gagtttcaaa gatgaaaaat tctatattaa
      541 ttttttgaca gcattagaga gagaaaaaag taataattca gataagcttt ttattataca
      601 ccataaagaa aaatatgaag gaaaacttcc catttgggtt gcaacggaaa taatgacttt
      661 tggtatgttg tcaaaattat attcaaacat gttgccagaa gatactagat acataaaaaa
      721 taatttgtgt agagtgaata ctttattagt taaatcttgg ttacaatcat taacacaggt
      781 cagaaatcaa tgcgctcatt atggtagaat atataataat aatttccgta ttataacaat
      841 aaaaaacgaa tataaaaagt ataacttgga taataaaaag atattttctt atatacttgc
      901 tatgaagcat ttgactatgg ataaattaat ttggaatagt ttttttataa aacttcaaaa
      961 gttaattaat gattataata attctataga cttaaagctt attggttttc ctaataattg
     1021 gatagagata ttggctaaat aaaatagtta ctttaagaag tttataaaca cttactatat
     1081 gtaagtgttt ttttattgaa aagaaggagg aaaataaata atgaaaatat gtattacagt
     1141 aggacacagt attttaaaaa gtggtgcatg cacttctgct gatggggtag ttaacgaata
     1201 ccaatacaat aaatctcttg caccagtatt agcagataca tttagaaaag aaggtcataa
     1261 ggcagatgta ataatatgcc ctgaaaagca gtttaaaact aaagcagaag aaaagactta
     1321 taaaatacct agagttaata gtggaggata tgatttactt atagaactac atctaaatgc
     1381 aagtgatggt caaggaaaag gttcagaagt tctatattat agtaataaag gcttagaata
     1441 tgcaactaga atatgtaata agctaggtac agtatttaga aatagaagag ctaaattaga
     1501 taaaagatta tatatcttaa atagttcaaa gcctacagca gtattaattg aaagtttctt
     1561 ctgtgataat aaagaagatt atgagaaagc taagaaacta ggtcatgaag gtattgctaa
     1621 gttaattgta gaaggtgtat taaataaaaa tataaataat gagggagtta aacagatgta
     1681 caaacataca attgtttatg atggagaagt tgacaaaatc cctgcaactg tagttggttg
     1741 gggttataat gatgggaaaa tactgatatg tgatataaaa gattacgtgc caggtcagac
     1801 gcaaaatctt tatgtcattg gtggtgcagc atgtgagaag attggttcta tgactaaaga
     1861 aaaatttact atgataaagg gtaatgatag atttgataca ctttataaag cattagattt
     1921 tattaataga taaattaaaa agtagcaact aaaagtagtt gttatctttc tgaaaattaa
     1981 gagttgtatc aataagttga gtctgattag taggaattta agaggtacat tttgataaaa
     2041 taacgaaaaa gcagaagtct catgaaaaga agaatattat ctaaaaagaa tggcattata
     2101 atcaataaag atgatataat taaaaaagta aaggtacgaa gatactgaaa attattgttt
     2161 aaagtggtgg taatatgaaa gatataatag agatatatga taattacaat aacataggcg
     2221 tgaaaaatag aacgattaca gaaggtaaag aaattgagtt agtaagagaa tatattaatt
     2281 ttagaaaaag tgagtttcaa ccaactgaaa atagaaagtt agcaattttt cttgaagcta
     2341 aggttgacaa cgcatatcca gatatagttt ttgttgaata ttcaccagaa aattatataa
     2401 actggaatga agcaagagca gaattgaata aagatgattt gaaaattcta tatcatattt
     2461 atattacagg aggaattgat tctacaggat tagtaaaaca attaggagta aattggaaag
     2521 aggcaatgtt agcaattgaa aaattaagtg atgcagacct aatcataaga aattataaaa
     2581 aatggaaaat aaaagatgaa aaaaaattaa caacttatac gatagaagct gtagaggcaa
     2641 agttgaacaa ttgggatgaa gtattgcaac agagtattat aaataagaat tttgcatctg
     2701 agagttatgc attatctatt atgaaatcaa aaccaaaaga agagacacta aaaaaattca
     2761 gaaagtttgg tgtgggggta tatttgaaaa atgaaaatgg gtttaaaagg attaaaaaag
     2821 ctagaagtgt tactatgcct gtaagtttta attctatatt ttttaatgaa tggataggta
     2881 ggattataaa cttaggttaa gggggtaaag tgttgaacga taaacagatt atagaaaact
     2941 ttaatgttag agaagtaata aaaagatttc ctaaaagtgg tcaaaaacaa gtcttcttag
     3001 ttaatcataa agtatatgga aaggttattt tgaagttggt gcaaagtggg gatgagcgcg
     3061 ttaaaagaga gattgaaatt gtaacagaaa ataattttta taatgttcca aaggtaattt
     3121 gtgttggtaa ttatgatagt tcagaagaat gtgggattta tattttagag caatatatcc
     3181 aaggaatgtc attaagggaa attatcaaag aaaaaagtat aacattaagt gaggagcttg
     3241 tgttggcaga aactatgttg aagattatag tgcaaatgga acagcaaaaa atagtgcata
     3301 gagacattaa acccgaaaat atattaagga acacagaggg agaatggtat ttaattgatt
     3361 ttggaattgc cagagcgtta gatatgagct cattaacatt tacaaaagtt gaaataggac
     3421 ctcatacacc tggatatgga gcaccagaac ttttccaata taataaaaaa gacattgata
     3481 gtagggctga tatattttct cttggtgttg tattgtttga ggcagttact ggttatcatc
     3541 cttttataaa aggagatgag atgaatttaa atgaaatttg gtataataca gttacagtaa
     3601 tgccagaaag tgttgttatt gaaggtgatt ataatatgca atatataagt ttaatacaaa
     3661 cctatatgca aaaacatgtt accagaaggc caaatacagc tcaaaaggct ctagaatggt
     3721 tttataatgt taaagataat ttattgaaag ggtgagaata gaatggaatt gtttttacag
     3781 tttgcatggg gtatgaaaca aatggtatta gatttatcaa aagattggag tggagctaca
     3841 actattttaa gtcctcgaga tatatcaccc aatcaattag aaagatgggt taaagaattt
     3901 aaaaaagcaa atgttaaaac attatttgac ccacagtgtt attatccaaa atgtaatcat
     3961 aaaaatttag ttcaatataa ctattgggat tcttcatttt ctacaaaaat gcaaggaagt
     4021 ttttcatttg aagatgaact aataaaatca attttatatt ataataacat tgcagaaaca
     4081 catgagttta ttattccatc aaatatgctt actcatgatg aaaaatggtt cagtagatgg
     4141 ctaaagaatt ctgaacaatt ggtaaactca gcaagaaaaa tagtaagaga tagaaaaata
     4201 atactaacat tagcaattcc agatagcgta ttgttgcaaa aagaggatga agtagaaaaa
     4261 ataattgaag caacagaaaa gttggatgtg gatggatatt acataattgc acatccacct
     4321 caagataagt acttagtaga tgttccaatg tggttgatta acttgacaca attatgtgca
     4381 ggattaaaat tacaaagtag aaaagtaata atgggatatg gaaatcatca attactttgt
     4441 ttgactgcaa ctggaataga tgcgatggca acaggtacat atttaaatgt tcgtagattt
     4501 actaacaaat ttgaagaaga tgatagtata aagagaaaaa gtatctggta ttattatcct
     4561 gctgcattat ctgaatataa aatgggtttt ttagatgctg catataacaa tggagtattg
     4621 caacaaatga gaccagctaa agaaatggat aggggatatg ttgacttgat attttctgat
     4681 gtgcttccat cttcaactgc atttaaagag ccaatggcct ttaaacatta tctaaattgt
     4741 gttaaagtac aaatgaagtc gttgttgaag caaacataca aagaaacaat tacagcaaat
     4801 gaaataatgt tggaaaatgc tatgaggcgt attgagtata tggaaaaaag tggggtatat
     4861 gcacagggga gaagttttaa agatatggtt gatgttaaca tgtcagcttt acaacgcctt
     4921 gataaaaata gaggattcca attacaacag gagtggaata atttctaatg atttatcata
     4981 tctattttct tatttaaaca aagtaaatga aatttggttt atataaggaa aagaagtgat
     5041 atttaaaaga gaatataaac ttagtgaaaa caattaatat tactgataga gatttcaact
     5101 gattttcaat gttgttttga taatagaatt aagaatttac taatttcatt tattaaaaaa
     5161 attgaaaaga acttaaaatg ggaaatactc tagataagaa cggtttttat aaattatcaa
     5221 aaaacatttt aaattgtctt taaaatcaat ttaaaaaagt tttttcatag tattaattat
//
LOCUS       DAKBNN010000232         5233 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000232 DAKBNN010000000
VERSION     DAKBNN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.238"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            26..862
                     /locus_tag="OUB02_003448"
     CDS             26..862
                     /locus_tag="OUB02_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8887175.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1215..2792
                     /locus_tag="OUB02_003449"
     CDS             1215..2792
                     /locus_tag="OUB02_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCT8887176.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2785..3969
                     /locus_tag="OUB02_003450"
     CDS             2785..3969
                     /locus_tag="OUB02_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8887177.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPIGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            3987..4586
                     /locus_tag="OUB02_003451"
     CDS             3987..4586
                     /locus_tag="OUB02_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8887178.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4610..5080
                     /locus_tag="OUB02_003452"
     CDS             4610..5080
                     /locus_tag="OUB02_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8887179.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1944 a          556 c         1012 g         1721 t
ORIGIN      
        1 agaatttttt tagaggggat ttatagtgaa tataaaaaag atatttaata acaatgtagt
       61 agtaagctcc ctagaagatg gcacagaaat aattgtcact ggtgctggag taggatttaa
      121 gaaaaaagta ggcgatttga tagatgaaaa tttgatttct aagaaatatt ttgttcaaga
      181 cgatcaaagg gacaaataca atcaaatact taataagacc tctattgaat actttaaaat
      241 ttcagaagaa attatagaaa aggctaatga agttttaaat actcaagtga atgattcaat
      301 aatattagcc ttaactagtc acatagaatt tgctgtccaa agagaaaaac aaggtataaa
      361 attaccaaac ttaatattga atgagacaaa acaactttat agggaagaat ttgaatttgg
      421 attatgggca attgatgaaa ttgaaaaaag gataggaata aaattgcctg aagatgaagc
      481 aggatatatt gctattcaca taatcaatgg tttggaaaat tctcaaaaag atgaaggtat
      541 taatatacta gagttttcaa aacaagttat tcagataatt gaagaagtac atggatttaa
      601 attggatgta aattcattaa attatactag acttataact cacttaaagt tttttgttca
      661 aaggattctc cgaaaagaaa cttacaatga tagtgatatt gaagatatgt ataagttagt
      721 gaataaaaat tacaataaac ctaaagtgtg tactgaagaa attgcagcat tggtattaga
      781 cagatttgaa tataagatta gtaaagaaga agaattatat ttaatgattc acataaataa
      841 aataacaaac agtggttgtt aggattgtaa ctgataaagc aggcaaaacc taagtcattg
      901 agcttaacta ttagattaag ttttttgatt taggtttttt tattagatac aatcaaaata
      961 cagacagtga cttttaggat tgtaactggt taagcaggca aaacctaaat cattgaattt
     1021 aactattagg ttaagttttt tgatttaggt ttttttatta gatacaatca aatacagaca
     1081 gtgactttta ggattgtaac tggttgagca ggcaaaacct aaatcattaa acttaattat
     1141 taaattaagt tttttgattt aggtttttct ataaatttga ttatgttttt agataaaaat
     1201 aggaggggaa aattatgaaa aaaaataagt taggtttatg gaacttcttc caaatgttag
     1261 gaaaaacgtt tatgttccca atagcactac ttagtgtttc tgggatgatg ctaggacttg
     1321 gggcaggttt tacagaccct aagatgattg aaatggttcc atttttaggg aatgaatatg
     1381 ttaacttagt tttaaacttc atgttaacaa taggattatt tgcatttaac aacttgggag
     1441 cattatttgc aatggcaatt ccactaggat tattaaaaaa agaaaaagag tttggtgctt
     1501 tttcaggatt agtaggattt atagcaatgc atataggtac aaatttttat ttaacaagag
     1561 ctgatttgtt agtaccagca gaacagatgt caactaatgg tcaagctatg attatgggta
     1621 tacaaacata taatacaagt gttttaggtg gtattgtagc aggattaatt gtttatgcaa
     1681 tatatgataa agttagtaat ttaaagatac cagaatcatt aggattctac agtggaccaa
     1741 gattagttcc aattgttagt ttaatagtta tgagtatagt tggattatta atacctattg
     1801 tttggccacc atttttcaat gcattccaaa gtttaggaag atggatttct tcagcaggac
     1861 cattaggata tttttcatat gcagtggcag aaagagttac aattccattt ggactaaatc
     1921 atcttgtaac atccgtattc cgttttacac caataggtgg aactgctgtt attgatggag
     1981 aaacgtactt tggtacattg aacatgttta tggcatatgt agaaaataat cagattatac
     2041 ctcttgattt agctggtaaa atggaacaag gtaaattgat gattcaatat ggattagctg
     2101 gtgctgcatt ggctatctac cgtacagcaa aaccagaaaa tagaaaggca cttaaaggac
     2161 ttttaatatc aggggtgttg acagttatta taggtggtat aagtgaacca atagagtttc
     2221 tattcttatt catatcacca gcattatttg ctttccatac atttatgaat ggtctagcaa
     2281 acatggtact tccatactta ggcgtgttga tgggattcac aggagattta attgcattta
     2341 taagttttgg tgtgttaaga ggaacagcta caggatggcc aatagcagta ttagtagcag
     2401 ttttatattt tgcaatatac tattttgtat ttaagtgggc aattattaag tttaatataa
     2461 agaccccagg acgtgaggat aaagaaataa aagtatctga aggaaatggt tcagatttca
     2521 aaaatttgtc tacatataaa ggacaacaaa tgatatctgc tttaggtggt caatctaata
     2581 tattatcact agataattgt gtaactagat tgcgtttaaa actacaagat gtatcattaa
     2641 taaatgagga tgcaattaaa gaggctggtg gtatagcagt tgttaaattg gatgagcata
     2701 caatacaagt aattatagga actcaagtgt attctttaag aaagcagatg gataaagcta
     2761 tggaaggtca tttggaggca tgtaatgatt aattttgata aaaaagtaga ccgattgggt
     2821 acatattgta cccagtggga ctatgtagaa gatagatttg gtaagaagga tttgcttcct
     2881 tttacaattt cagatatgga tttagagttt ccatttgaaa taacagaggt gttgcaggaa
     2941 aggttaaatc atagagtttt gggatacagt agatggaaac atgatgattt tagaaattca
     3001 gttacgaatt ggtatattaa aagatttaat tgtagcgttg atagtaactg gatatattat
     3061 agtccaagtg taatgtattc aattagcaaa cttattgaga tgttaagtaa tgaaggagat
     3121 ggaatcctta taaatactcc tgcatacaat gctttttatg atgtgataac aaataacaat
     3181 cgtaatatta taaagtcaca tcttgtaaat aggaatggat tctattcaat tgattttaat
     3241 gattttgaga aaaagtgtaa aaaatctaag atatttattt tatgtaatcc tcacaatcca
     3301 attggtagag tatggagtga gttagaactt gttaagatga taggaatttg taagaataat
     3361 gatgtcaaga taatatcaga tgatatacac atggatattg tatatgacaa tagaatgaca
     3421 cctatcttaa atgttgcaaa ggactattta gattctgtat tcatttgtac atcagcgtct
     3481 aaatctttca atataccagc attgactggg tcatatgtga ttataccaaa tgaaggtatt
     3541 agaaataaat ttgaaaatat cactagatac cgtgattttg taaattcgcc agcaattcta
     3601 gcaatattag ctacaatggt ctgttatgat gactgcgaat attggttaga tgaactatta
     3661 aaatacctta aagagaatct taaatatacg attgattata taaaagataa tttagaacca
     3721 ttgaaattgg tcatgccaga aggctgttac tttgcttgga tagatttttc cgaacttaaa
     3781 ataagtggtg agaaattcca aaaattattg atggatattg gagaagttgc tattatgtcg
     3841 gggtctgtat atggtgaaga aggtgagttt tatttaaggt taaatgtggc atgttcaaga
     3901 gaaaaacttg aagatggttt aaataggatt aaaaaaacta tggattatat tgcaaaaaga
     3961 attttatagt aggaagggag atagttatgg gatatttaaa aacaatggtt gatgttatga
     4021 ctactgaaat gaatgccata agatgtttaa tagatgaagc aggaatagaa tatgaaagta
     4081 tagttagtga aattgcaaat tgtgaaggca aagtaatttt tatgggtgta ggaaaatctg
     4141 cacatatagg taagaaattg gcagctacat ttgctagtac tggtacacct agcttttttg
     4201 tacatgcaac agaggcagtg catggagatt taggaatgat tgaaagtaag gatattacaa
     4261 ttttaatatc aaatagtgga aattctatgg aagtggtaaa ctgtataaaa tatattaaag
     4321 caataggttc taagacaatt gcatttactt caaacagaaa ttcagtactg gcaaaagagt
     4381 gtgattatgc tttaatatat cctgcaaagg atgaagcaga ccatttaaat ctagcaccaa
     4441 caacatcatc tacaattact ttggttttag gagattctat tgcatgtgca ttatctaaga
     4501 gtagtaattt tggttcttca gatttttata agtaccatcc aggaggaagt ttaggtgaga
     4561 agttaaaaac agctaacaat ggataaaagc aaaaaggaga gtagttttta tgttcaaaat
     4621 gtttaaaaaa aaatcaataa aatcacccat atcaggaaaa gtaatagagt tatctaaaat
     4681 acaagatggt gtattttctc aaaaaattat gggagagggt gtagctatag attctacagg
     4741 agacattgta tatgctccaa tcaatggaaa gatagtagta gtagcagaga ctaaacatgc
     4801 atttgtaata gaattagaaa atgggatgga acttcttatt catgttggtt tagatactgt
     4861 aaatttaaaa ggcgaaggtt ttgaggtatt ggtgagtatg ggagaacaag tgaaggaagg
     4921 aacaccaatg ttgaaaatta accgtccttt gattgaatca aagggtatat cacttattac
     4981 tccagttaca ataactaatc attcagagta caatatgaat acatgtaatg taggcaatag
     5041 tgttgaaggt gggaaagata ctgtaattga gtttaaataa agaactaaaa tacaaggtta
     5101 atatttataa atacctataa acttatgttt tataagattg attgtataga tttgttgtat
     5161 aaaaaagaac tctttaatta taaattaagg agttcttttt tattttgtaa atattgttta
     5221 gatttatata gaa
//
LOCUS       DAKBNN010000233         5213 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000233 DAKBNN010000000
VERSION     DAKBNN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.239"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(460..1332)
                     /locus_tag="OUB02_003453"
     CDS             complement(460..1332)
                     /locus_tag="OUB02_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCT8887180.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1726..2304)
                     /locus_tag="OUB02_003454"
     CDS             complement(1726..2304)
                     /locus_tag="OUB02_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCT8887181.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2362..3078)
                     /locus_tag="OUB02_003455"
     CDS             complement(2362..3078)
                     /locus_tag="OUB02_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCT8887182.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3269..4354
                     /locus_tag="OUB02_003456"
     CDS             3269..4354
                     /locus_tag="OUB02_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCT8887183.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4509..5168)
                     /locus_tag="OUB02_003457"
     CDS             complement(4509..5168)
                     /locus_tag="OUB02_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8887184.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
BASE COUNT         1606 a          811 c          547 g         2249 t
ORIGIN      
        1 aaagtttccc tttccccaaa aatggctact taaaaaatca agatttcata cctaactact
       61 aattctatga tttttaaatt tttcaacttc tttgtcaaag ttaaaataaa atactaaaag
      121 acacatatta tagtgaacaa aaaagataga aatacatatt ttaatataat tttaataaga
      181 tattatatac ttaaatacaa ataaaattaa ttttataatt gttaattaag gaaatgatat
      241 tgtttagaga aataaaaaaa gcactctcca tgaatggaaa tgctttcttt aataaattag
      301 ttgacgtagt aaagatgtat tcagaataca tctttatgtt ttgagtctcg aaatatttat
      361 ttgtattatt taaacgaaaa tttatttttt aagtgataat cgagtattta ctaataaggc
      421 tatgattctc aatttcatag ccttattaat atacaaactt tactcataca cagaatatct
      481 tttccaaatt tgctctaaat catcaaaatg ctgtttaata tcttcttttt ccttcatccc
      541 aacagatata gcaagggcat ttgaaatact aagagctgga actagagaat ctacaaatga
      601 agccatatta ctcttaacta gaagagtgtt atctgcaagt gatgctactg gcgcaaataa
      661 gctatctgtt aatgatatta catgggctcc tttttcctta gcataattga caatttgata
      721 agatttctta gaataacgag ggaaactaat agcaactatt acatcttctt catttattct
      781 tacaatctgc tcaaaagcat ctcccatatc cattctaatt atatgaacat tgtcaagtat
      841 tatatctaag taaaaaccta aatattgagc tactacaaaa gaactcctca ttccaagtat
      901 atatattttt ctcgctctta aaagtttgtt agaagcttct ttaaacgctc tctcgtcaat
      961 ttcttctagt gtagacctta tattatctat atcacttttt aaaactttgt ttagtattgc
     1021 aaaatcatcg gaataatcat gtgccatttc aactctttgt actgtagtta gtttattttt
     1081 tatgagttct tgaagtgcag cttgtaattt aggatatcca ctataaccta atgcatttgc
     1141 aaatcttaca actgtagact cacttactcc tacagtttca cccaatttac atgcagtcat
     1201 aaatgcaact ttatcataat tatttaaaat gtattgtgct ataagttttt gacccttact
     1261 caatctcgta tattgagatt gtatatttga tatcaaatgt ttactgtcct tcatttcatt
     1321 tgtttcttcc atttttttct ccttttagtt atataaacta ttttgtttta ttaacactgt
     1381 ctttttctct aaaaatttta ttttttatct caatttcatc ttagttttta tcatagcttc
     1441 ttaaactcat aaaattctaa cttttctgta ccttatactt cacatagtat tttcgattaa
     1501 atttattata ccatctttcc ttgcattttt taatatttat ccaatatttc ttttaaattt
     1561 ttcaattttt actcatttaa aagttatcta tcttattttg catatttaga tgtatatttt
     1621 gttttgagaa taagctgttg tatacttatt atttaaagta tttttaatag gtcacactat
     1681 atatattaac tcttaataca atcttgtcaa gttttttagt atttattatg cttttttctt
     1741 ttcaattaaa atcaattgtg gtgggttatt gacttgattt aaaaacttag attctaaaac
     1801 attaaattca tcttgattta aattattgac aaggttatat atgcaatctt tttcttcttg
     1861 acctccatca tgccctgtat atatggcaat gcttattact ccattttctt ttagcatttc
     1921 taaacttaac tttatagcct ttaaagttgt atctggtttg gttataattg aatgtttcgc
     1981 tcttggaaga taacccaaat taaataaaac acatgaaact tcttctttta tatattgatt
     2041 cattttttca tgcccatcaa atatcaattt tactctatct gtatatcctt ctttctctag
     2101 tttctttctt gtagacttta ttgcttcttc ttgtatatca aaagcatata caaagccctt
     2161 ttctccaacc ttctcagcta gataccttgt atcataccca tttcccatag tagcatcaat
     2221 tactatgtca ccttcattta taatattttc taaataaaat ttatttatat ctgtaatttt
     2281 agttaaatac tttgcatatg ccataatact atttctccct actattaact ttatttcatt
     2341 ttttctcaat ttatttttat cttattttat tgattttaaa tattttattt catcttctgt
     2401 taggtatcta tattctccaa ctttggtgtc ttttaatact attttcccca cagaaatcct
     2461 tcttaaattt aaaactggat gatttatagc tttgcacatt tttcttactt gtctatttct
     2521 accctcatgt atagttattt cacacactga ataatttttt tctttatttt ctttagttac
     2581 ccttattttg gcaggtgccg ttgtgtaatc ttctattttt agtcctgttt cgaattgtct
     2641 tatctcatca tcacttataa ttcccttcac acttgccaca tacgttttat caatttcatg
     2701 ttttggatgg gtaagcttat atgttaaatc gccatcatta gtaagtatta ataaaccact
     2761 agtttcataa tctaatctcc ctattggata tactctttcc cttacatctt taagtatgtc
     2821 tattacactg ggtctattga attggtcttt tactgtagtt atatatccct ctggtttatt
     2881 taatactata tatatataat tttcacttgg ttttactttt ttattatcaa attctactat
     2941 gtctttttct tcatcaatat taaaagataa ctcttttact acacttccat ttacctttat
     3001 tcttcctcca agaattattt cttctgcttt tcttcttgat gctattccac aagaagctat
     3061 atacttatta attctcatac ttaatccttc cttatcttaa attatgtgtg tttaatattt
     3121 ttatcaatgt taattttgct acattattat actatactag accttaatta aaaacaagca
     3181 aaaaaattat ttaattttcc taaataattg taatttcttt tcacatacta ttaatatgta
     3241 atagctgact aaacactatt ttaaggaagt gattattatt tcaaaggaaa atataaaata
     3301 ttatttaata gtcgtgttta ttggaatttt attttttaaa tttattaata ctccatcaga
     3361 ctttatatct agtattgaag gattcttaaa gttttttagc ccatttttat tagctatttt
     3421 acttgcttta cttttaaatc cacttgtgat gctttttgag gtaaaattca aagcacatag
     3481 acttttagcc atttttttat cttatatatt tataggtttt attttagcct ttgccattag
     3541 gttattaatt ccatcaatag ctaatacatt aaatagatta ataaatgaaa tgcctatgta
     3601 tactgattat atagatagtt ttatagaaaa aaatatgtca aacatagatt ttctaaaaac
     3661 tttaattcct catatacaac atagtttgga taatttatta aaagaagcaa gtaattttgt
     3721 tggtaaaatt cctaaaaact tcttaatcta tacacttagt atatcttcta tgctatttaa
     3781 tatgacaatg ggatttatac tatccatata cattatatat gacaaagaaa aaattgcatt
     3841 gggatttaag agatttctat attcttctac tgctagaaac aaggcagata acattataga
     3901 attttttagg actactcatg atattttcta tgattattta cttggtagaa tattagactc
     3961 tcttattatt ggtataattg catttttagg atttcaattt gttatcagaa tagaaaatgc
     4021 tttattttta gcttcaataa tattcttagg taatataatt ccctattttg gtccttttat
     4081 tggtgcaatc cctccaatag ccatgactat attatatagt ccacaaaaaa ctatatgggt
     4141 cattgcattt ctatttatat tgcaacaatt agatggaaat tttatagaac caaaagttat
     4201 gggtaatcaa gttggtatag gagctatatg ggtaatatct gctattttgg tgggacaatc
     4261 tctatttggt ttcataggag tttttctttc agtaccaata gctgctgttg taaaaactta
     4321 tgttgataaa tatatagata atcgtttaca atagattatc tctactgtga ctttaatttt
     4381 tagtttaaaa tttaactgct atttattaaa ttatttctca aaaaatatct cttagtaaga
     4441 aaaaattgct aagagatatt ttttataata tataatttgt tcattaaatt tatgcatata
     4501 ttttatactt attgattatt tgagttttga gaatttccac cactatctga accaccagta
     4561 gtatcttgtg gagttggtgc tggtgttgga ttactattat ttggcttttc ttgattttga
     4621 gaagaactgt tattattgtc agacttatta ctatctgatt ttttatcatt gtttttagaa
     4681 ctagatttat tatttttact acttccacta taagaagatt tccttgcatc tgattcaact
     4741 ctatacctat cttctccagc cgtatctttt gcctttttct ttctttgttc ctctgcttcg
     4801 ttttgttctt tttctaattt agttaactct tctttctttt tagttgcctc ttctttaata
     4861 acagaggaac catttttgat tttctctata agtttaagtt ccttgattgc accctcataa
     4921 ttttttaatt cttcaaactc cgtcatattt gacattctca tagcttgcat atacatagct
     4981 cttgcattct cattagtact atcttcttcc agtgcagagg ataatgattc catagctttt
     5041 ttatagttat gtttttctaa atatgttttt ccttgctcta ctaaatttat ttcatctttt
     5101 ttattaaatg aacatcctac aagaaatata gataatatcg atattaataa aattactctt
     5161 cctctcatta aatccctcct tgtcatatac tttaaatttt atcataatta taa
//
LOCUS       DAKBNN010000234         5170 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000234 DAKBNN010000000
VERSION     DAKBNN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.240"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(40..1713)
                     /locus_tag="OUB02_003458"
     CDS             complement(40..1713)
                     /locus_tag="OUB02_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HCT8887185.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(1723..5121)
                     /locus_tag="OUB02_003459"
     CDS             complement(1723..5121)
                     /locus_tag="OUB02_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCT8887186.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSIYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
BASE COUNT         1597 a         1005 c          622 g         1946 t
ORIGIN      
        1 taaactaata atcaattata attttacata ggttcttatc tatagagtat ttgctatctg
       61 ttgaatcgta ttttttaaat ttaaaatccc atatccctgc gatacatttg gatatgtata
      121 tatctcaagt tttgtagcac ccaatattaa atatgttttt aaaacttgtg taaacaatga
      181 taatctcggt acattatcct gtttttctaa gtattccatc aataaggcta aaacacctgt
      241 gactatagaa ctgctcactc ctgttcctgt tcctgtatta tatgttccat ttttatatgt
      301 ggatattata tctacacctg aagctactat atctggctta attcctcttc ctctaattgg
      361 tcctttcgaa gaaccaatcc acatactatc agtcttattg ttaaatgtac caactgtaat
      421 tacatcatca ctagcagcat acatagttat agtagctact gaatcagggt ctaaaaacct
      481 tgtgtctgga gctatcaaat ttttatttgg caagtatatg tcatattctc catttattat
      541 aagttctgaa gtcaatctta aaatccatac tccaggtttt atatctctta atcttatctc
      601 caaattttct tttccagagg taatatatgg ataaataaat ctcattgcat aagtagtatt
      661 ttctaagtta aattttcctc tatatatata aaagtctgga gaatacctta tatcatgact
      721 aacttcacct gaaggagata ttatctgtgc tcccacttta tctggaccat tcgtatttag
      781 tgttatatca agagcatagt catctccatc ttgtattatt acatcttgaa cttctcccac
      841 actgctaaat ctgccagaat aatgaatatc tgtattccct tgatttcctg caccactcac
      901 tactaccaca ccagcactac tcaatatatt aaaagtatct agtattgaag tagttgctac
      961 agcacttgac ttaactccaa tagtcaaatt tattatcagt ggtttatttt ctgttcttgc
     1021 tatgttagta acatacgtta tagctgctaa aaaatctgaa acactgtaat ttatccttcc
     1081 agcataataa gtatctgtat atgattttaa tttaactact attaagtctg attctgtagt
     1141 aatacctctg tactgagaat ttatccttcc atttccaact aatataccag aaactaaagt
     1201 tccagtacct atattatctt gactcaaact cccatcattt ctatttatgg ctatatttaa
     1261 ctcacttctt gtaaattcac ttccaaaaat aaaaccttct ggaggtggat ttgtatttgc
     1321 ttcttggtcc cataaatata aaactttact agttccatca tcatttataa aatcaggatg
     1381 caaataatct atcccagaat ctataactgc taataaaatt cctctccctg ttatatcatt
     1441 atatggattt tcataaatat aatcagtttc agctgctgtt gtaatggttt ctccattatt
     1501 tacattatta gttatttcaa ttagactgct cattggttca gattcttccc accaagcaac
     1561 ttgtattata ttatttaaaa tagtttcatc aaaatctaaa ggtacatata ttactgcaag
     1621 ctgactattt aaaaccatat acctatttat tccattttct tgtagggcag attctatatc
     1681 accttgataa attatacaat aagatttttc cataaatccc tcctatctta aaacatcaaa
     1741 cattcctcta acatccaaga gcccatatcc agaatttgta tttggatata ctgtgtttga
     1801 atcttttcta gcaccagctt gcataaaagt ttttattttt tgcacatatg cttggtttgg
     1861 atatctaccg tcaacaaaag tatattgaaa atacattgca gcagcaccag ctgcatgtgc
     1921 acttgctgct gctgtaccag ttatagttgc atacgtgttc ccaggataag cagctattat
     1981 attaacacct ggtgctacta tatctggttt taacctgtct tcaatagttg gccctcttga
     2041 tgaactttgc cataaactac cattaatagt attgtaggct cctatggtta ttaaatcatc
     2101 ttgaatcgca ggataattta tagtgtaaaa agggtctacc tctctaaatc tagttccact
     2161 ttttaaaagt tctctatttg gaaggtataa gttatatctt cctgttataa tataaactcc
     2221 tactaatcga atcttccata cacctctttt tgcattcttt aaagtaacat tagtaaattg
     2281 ctgtccagaa aaagttgttg ggtatatata tgttatagaa tattcagtcc cctctaaatc
     2341 aaataaacct gttactttat tataatttga aattccaaca cttttacttt cttcaccagt
     2401 gggagatact attattacat cagctttatc tggtctattt aaccaaagtt ctaatgataa
     2461 ttcttcctcg tcttcattta actctaattc aacttcaaca gaacctccaa catatggtat
     2521 tattccagat gtatgtgtct gtgtattgcc ctcatttcct gctcctgccg ttatacataa
     2581 ccctctagta aagaacgctt tttcactatt actcctattg gttaaacctg ctaggctact
     2641 ggtacctaaa gacatattta taactaatgg tcttccaagt tcaaaagctt ttttatatgc
     2701 atactgagat gcagcaaaca acattgcact attataaaaa tcatctatct tgccgagttt
     2761 tattattatt agctcagaat cttctgctat acctgcatat tcactattta catttccaag
     2821 ccctgcacat atcccactca acatagttcc ttgaccaact tcatcctgag atagactagg
     2881 gtcattttct gcgatagctc gatttatatc ttctcgtgta tattctgtcc caatataaaa
     2941 accatcagga ggagttcctt cttttgtttg gtcccataaa tatactattt ttgaagtgcc
     3001 atctggataa ataaaatctg ggtgtagata gtctattcct gtatctgcaa ttgatattag
     3061 agtccctctt cctgttatat taatattagg attattttta aagaaattta cgcccatttc
     3121 ttctgttgca actacacctc caaaagtacc tcgattaatt tctccaagta atgtcatctt
     3181 tgtagtcgat acagttctta tgattgaagg aagttgcaaa actcgttgta tgctattata
     3241 gtctgtatta tttatagata gtacacctaa tgaatcactt atcttaaaaa acctttgact
     3301 catacctaac ccagctagtt cttcttcaaa ttcaggagta tggactatat ctattcctga
     3361 tagtattcct gattgtctac ttgcattttt acttatataa atttcattct ctaagtcatt
     3421 atgtatttgc atattataac catctataaa cttataacta ttttcctcat ctttaaatga
     3481 cttattaata cttctatatc caatatcctc tgtttctaaa tcttgattta tattagatag
     3541 agtgtacaaa tctatatctg ataaatttaa aaaaccaaat cctgatgaat tatttggata
     3601 agattgatta cttaatctcc ttgctccctt tagaagtaat gcccttaatt tctgtgaata
     3661 caaaaataaa tcatttccat ttacaattcc ccattccata aacaatgaac aaacccctgt
     3721 aacatgaggt gtagccatgc tagttccagt taatgctcca cttgtacctc ctggtaaaaa
     3781 agatactata tcttcacctg gagctaataa atcaggttta aaaacaccta attgtgtatc
     3841 tccttcacca gaaaatatag atactatatc tgttcttgaa ttaaaactac ctactgttat
     3901 aactctactt gctgttccag gaactgttac agtaagctct tgagtaggaa tcagaaatct
     3961 agtatttcta tttagacctt cagaagttgg caaatatata tttacattac cagttactat
     4021 atctattggt tcaaatacaa ttttccataa cccaggagtt atctgtgtat ttgaacttaa
     4081 ttgtagagta actcttcttg tgagagaata cggtgctata gggtaaaaat aaccagttat
     4141 tctagtttca cccaaagtat tccttatctc acctgaagtt agagagattg cttgtgtttg
     4201 attatttgat gggtttacca aatgtacact aaaatcatca acaaaatctg gccatatatt
     4261 tatgtttaat atacgctctc cttccccaac tataaactca acttcttctg taatattatt
     4321 ttgaagccta atccttttat gacctccttt atctgcatta tttccagctg ctacaactat
     4381 attgtttttc caaaataaac acatatcatc tatatattgt tcaaataaag atgttcctct
     4441 atgtgaacct tcattacttc cataacttat atttaatgta acaggcattc ttaattctaa
     4501 tgccctatct aaaataaatt taattgccct cataaactct gtacttctcg aaaaaatatc
     4561 ggtctgtata tttccaactc taaccactat tattctggca tcacttgcaa tagttgcaca
     4621 aatacctgca acatgtgtcc catgtaagct agttgttgaa ataggtatat atatacttcc
     4681 atctattgca ttatttatat cttcattagt atataatgtt ccttctctaa aaccttctgg
     4741 tggatttcct tgaatagatt ggtcccaata atataatatc ttagaccttc catcactatc
     4801 tctaaacaca ggtagagtat aatctattcc cgaatcaatt ataccaatta tagttccttt
     4861 tccagttaaa ccagttctat ttttaaatcc tgttattcct gtacttgaaa aactttgcac
     4921 atcctgagtc tgtaatataa aaggcttttc tataaattca atttctggat aagttaaaag
     4981 tatatttaca tcttcttcat tacttgatgt tattattgca tatgatgaat taagtatctc
     5041 tactgataca cctaattctt cttctaacct caatatatct ccattgtatt ttacaattaa
     5101 ttcataattt attataatca cctcgacttc taaaattatt attaatgtat atgttttgtg
     5161 tgagtctaaa
//
LOCUS       DAKBNN010000235         5132 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000235 DAKBNN010000000
VERSION     DAKBNN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.241"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            46..249
                     /locus_tag="OUB02_003460"
     CDS             46..249
                     /locus_tag="OUB02_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887187.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            413..1567
                     /locus_tag="OUB02_003461"
     CDS             413..1567
                     /locus_tag="OUB02_003461"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCT8887188.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1580..2584
                     /gene="rseP"
                     /locus_tag="OUB02_003462"
     CDS             1580..2584
                     /gene="rseP"
                     /locus_tag="OUB02_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCT8887189.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2766..3821
                     /gene="ispG"
                     /locus_tag="OUB02_003463"
                     /gene_synonym="gcpE"
     CDS             2766..3821
                     /gene="ispG"
                     /locus_tag="OUB02_003463"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCT8887190.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4169..4993
                     /locus_tag="OUB02_003464"
     CDS             4169..4993
                     /locus_tag="OUB02_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887191.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         2007 a          521 c          987 g         1617 t
ORIGIN      
        1 caaaatagat aaaatgtgat agaatatatt ttgcgaggtg ataacatgat ttcatacgct
       61 ccattatgga aattgttaat tgacaaaaaa ataaagaaaa tggaatttgt aaatatatca
      121 ggtataagta tttctgtttt aggcagacta ggaaatgata aatctgtttc tatggataca
      181 atggagaaga tatgtttagc actagactgt aaaatagaag atgttgtgga aataaaaaag
      241 ggcatttaat aatactagaa tattttataa tattccacaa aatacaaaaa ataatagatt
      301 aattttaatc tgtggaataa tatatgaaat ttctatgaat agtaaaaata attgttttag
      361 tagtgttata atgttttgac agggtatatt tattattaga aaggattatt atatgaaaaa
      421 gatatcaatt ttaggttcta caggctctat aggtaaacaa actttggatg tagttagaga
      481 taatagagat aagtttgaga tagttgcaat atcagctaac agtaatattg aattattgct
      541 agaacagata gtagagttta aacctaaata tgtaacagtt tttgaggaaa ataaagcatt
      601 gaaattaaaa gaaatgttgc ctaaaaatat agaaatagaa gttttagcag gaatggaagg
      661 attaaaaata atatcatcac ttgatgaagt ggatgtcctt ttaactgctg tagtaggtat
      721 gataggatta gtcccaactc tttgtgctat aaagaaggga atagacatag cattagcgaa
      781 taaagaaacg ttggtaactg ctggtgaact tgtaatgaaa gaagcagaaa aatacaatgt
      841 aaatattctt cctgttgata gtgaacatag tgctatattt caatgtctaa atggagaaaa
      901 taaaaaaaat atagaaaaga taatacttac agcatctggt ggtccattta gaggaaagaa
      961 aaagggagaa cttgcaaata taactaaaaa tgaagctttg aagcatccaa attggagtat
     1021 gggaagaaaa ataagtattg attcttcaac gcttatgaat aaaggacttg aagtgataga
     1081 agctagatgg ttatttggag tagaacaaga aaatatagat gtagtagttc acccacaaag
     1141 cataattcat tcaatggtgc agtatacaga tagctcaata attgctcaat taggatgtcc
     1201 agatatgaga ctaccaatac agtatgctct tacatatcca gatagaatgg aaagtagctt
     1261 tgaaagaatg aatttttcaa aatttagtac tttaactttt gaagaaccag atttagaaac
     1321 ttttccatgt ttaaaattgg catatgagtg tttaaaaatg ggaggaactt attcttctgt
     1381 tttaaattca gcaaatgaag ttttagtaag tgaattttta gaggataaaa ttggtttcta
     1441 tgacatacca tattacatag agaagacttt agaagttcat agtagcataa gtgaaccaac
     1501 attagaagaa atcttagaaa cagatagatg gagtagagct tatgttgcaa atctgataaa
     1561 aaaataggaa ggtgaattta tgactataat agccgcatta atactattta gtataattgt
     1621 attaatacat gagttaggac attttatatt tgcaaaaaga agtggtatca aagtaaatga
     1681 gttttctata ggtatggggc ctaagatata tagtgtaaaa aaagatacag agtattctat
     1741 aagagcactt ccaattggtg gatatgtcag catggaaggt gaagatgaag aacaaataag
     1801 tccaaattct tttggaaaca agtctatatt acaaagattt agtactattg tagcaggacc
     1861 aatattcaat ataatattag cagcaatact tttagttcca gtatttttgt atataggttc
     1921 tccaacgact aaattaggaa agataatgcc agatactcct gcacaggctg tagggcttca
     1981 agtaggagat aagataaata aaattaatgg taattctgtt aagacttggg atgaagttgc
     2041 aaatataata aatacttctt ctggtggaga attaaaactt agtataacta gagatggaag
     2101 tgataaagtt gtaaatgtaa cacctaaaaa taacaatgga aagtatgaaa tagggattca
     2161 accacaaaga gaaaaagact ttttggggtc aatagtaaat gcatgtaaaa ctactgtaga
     2221 tatgacaaaa cagatgctaa catttctagg tcaaatgatt acaggacgtg taccaggagg
     2281 gattggcaat gctgtagcag gaccagtagg tgttattggt atggtatcag atgctgctcg
     2341 tacaggcatc ataaatgtag tgtatttagc agctgtcata agcttaaatt taggtatagt
     2401 aaatctatta ccaataccag cacttgatgg atggagaata cttatgctgt tactagaagc
     2461 tgttagaggt ggtaaaaaac tcgacccaaa taaagaaggc atgataaatg ttgttggttt
     2521 tggagcttta atgttattta tgctttttat cacatataaa gatatactaa gattatttca
     2581 atgatttagt ttttagagat tttaagaaaa taggctatgt agttttaaat aagaataaat
     2641 taatttttat atatgaaatt tattcttatt taactagata gctttttaat ttataatata
     2701 catgtaggtt ataatatttt aatatttaaa gagattcaat ataaaataat atatagaggt
     2761 ggaatatgag ttacgagaga agaaagacga gagaagtaag tgtaggaact gtaaaaatag
     2821 gaggagaaaa tcctataagt atacaatcta tgacaaatac tgatacaaga gatgcagatg
     2881 ctacaatagc tcaaataaag agattagagg aagctggttg tgatatagtt agagttgctg
     2941 ttcctgatat taaagctgct aaaaatatag caaagataaa gtctagtgta aatataccaa
     3001 ttattgcaga tatacatttc gattataagc tagcccttga agctatagaa caaggtgtgg
     3061 atggaataag aataaatcct ggtaatattg gaagtataga gagagttaag atggtagttg
     3121 agaaatgcaa ggaaagaaat ttaaaaatta gaattggagt taatggaggt tctttagaga
     3181 aagaactttt aaaaaagtat ggctctgcaa ctgctgaagc attagttgaa agtgcaatgg
     3241 gacatattaa gattcttgaa gacttagatt ttcataatat agttatatct ctaaaatcat
     3301 ccgatattta taaaactgtt gatgcatatg aattaatatc aaaaaaagtt gattatcctc
     3361 ttcatattgg aattacagaa tctggaagtg ttcacaaagg aacaataaag tcttctatag
     3421 gggtaggagc acttcttctt aaaggaattg gagatactgt tagaatatct ttaacaggcg
     3481 accctgttga agaggttatt gttggtaaac agattttaag aagtttggga cttttaaatg
     3541 ataagattaa agttatatca tgccctacat gtggtagatg taatatagac ttaattagtg
     3601 ttgtaaatga ggttgaagaa aagattggta gtatggaaaa aaatatcact gttgcaatta
     3661 tgggctgtgc tgtaaatgga cctggtgaag ccagagaggc agatataggt attgctgggg
     3721 gtaaaggtga aggtctttta tttaaaaagg gtgaaatagt tagaatgatt gatggtaaca
     3781 aattagttga tgagttgcta gaagagattg aaaaattata gtatacaatt catatatata
     3841 ttaattgact agatttttaa atacttttgt aataagtttt tatttacagt atttaattat
     3901 ctagtcattt tttattttat aaacttcatt aaatgtaaga aacttatatt ctaatttgac
     3961 tatatatgta tgagttaaat catattgtaa atttataaaa ttaaaaaaag atttaatata
     4021 gtgaaaattt ccatcaatag tattgaaact tgatagtaac tatgatagga tatagttact
     4081 agaacagttg gtaaattatg ttaacataga ttttaatgtt ttggtaaatt gttttattat
     4141 aagttaatag aaaaagaggt gaagaactgt gagatataaa aaaatatcta gcttacctat
     4201 tttagttgtt gctttgctga ttactggaac gacaacaaca ttttctctta gtacgaccaa
     4261 ttcagaaaaa acaaatgtat acttagcaaa tacggtaagt actgtcacaa agaaggtatc
     4321 agatattcaa agtagtaaag gtcttgttta taatgagaat attccattat ttataaataa
     4381 tagcaaaata cagtatgatg atactccata tcattggcct agcaatgtta taagcttaac
     4441 aaatagcagt gaaaaggcaa ttatggatta tgaaattaca tgcttagcat atgataaaaa
     4501 tggaaaacca cttgagctat attgggatgc tcaaaatgtg gcagcagatg gcgaagtagg
     4561 aagtgttgga tttagcccag ctggtgtaga ctatggtatt gttacaggca tatctcctgt
     4621 gtctccaaaa tcatattcac atacttatag aaaaatgcaa caatcaccac cacaggatat
     4681 aataagtatg tttgaaaaac aacaaggcaa ggcatgggta gaaaattggt tgaaagaatg
     4741 gaaacaaatg gagaaagaat atgcaaagca aaatgcaata gcaccaggta agaatcaaaa
     4801 tgatgccttt ctattgtttg ataaatggaa acaatcaact ggagagcatg gtgtaaagta
     4861 cattatatct tgtgtaaaac aagtaacatt taatgatggt agtgtatgga aaaactcagc
     4921 atatgagaat tggttgaaaa gttttcaagg gaaggaagtt tcaaatagtg tattagaaaa
     4981 ttactataag taatgtttaa tgatagcaaa atgagtacga atttaattaa tgttaattta
     5041 attaaataag gactattttg tttaaaaata tcagtgttac aagtaaattt ttattgtgac
     5101 aaaatgatat atatacataa gattataagc at
//
LOCUS       DAKBNN010000236         5114 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000236 DAKBNN010000000
VERSION     DAKBNN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.242"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(8..886)
                     /gene="speB"
                     /locus_tag="OUB02_003465"
     CDS             complement(8..886)
                     /gene="speB"
                     /locus_tag="OUB02_003465"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCT8887192.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(876..1727)
                     /gene="speE"
                     /locus_tag="OUB02_003466"
     CDS             complement(876..1727)
                     /gene="speE"
                     /locus_tag="OUB02_003466"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCT8887193.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(1757..2170)
                     /gene="speD"
                     /locus_tag="OUB02_003467"
     CDS             complement(1757..2170)
                     /gene="speD"
                     /locus_tag="OUB02_003467"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCT8887194.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(2257..3732)
                     /locus_tag="OUB02_003468"
     CDS             complement(2257..3732)
                     /locus_tag="OUB02_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCT8887195.1"
                     /translation="MDSEFANVVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(4370..5023)
                     /locus_tag="OUB02_003469"
     CDS             complement(4370..5023)
                     /locus_tag="OUB02_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8887196.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPMEEMKKEKLL"
BASE COUNT         1597 a          882 c          541 g         2094 t
ORIGIN      
        1 aaatactcta tttaatttta tcagatatta ttaagcataa ctctcttaag attttacaag
       61 ctataactgt cgatacacct gttgtatcgt aatcagggct taactctaca atgtcacaac
      121 caactatatt tatattagag ttttttataa tcttaaaaat ctcttgaaac tctctataat
      181 ttacaccacc aggttctggt gtacctgttc ctggaaacac agatgcatcc aatacatcta
      241 aatctatagt tagataaata ttctttccat ttagcatatt aactatattt tcaaacgtat
      301 ctattcctcc tatttccatg tatgtgtgtt tttcttctgt agcaaattta aattcttctt
      361 ttgtcccaga ccttatacca aattgaaata ttttattatc acctacaata tcccatattc
      421 ttttaattac tgttgcatga gaatttttac tattattata ttcttccctc aaatcagtat
      481 gggcatcaaa atgaattaca tatatatcat tgtacttttc atgtactgct ttaaaggctg
      541 gtaatgtaac taaatgctct cctccaatca taaagggtac ctttgaatct ctaacaatct
      601 tatatgtctc ttgatagatt tcttttaaga cttgttctgt acttccaaca ctaatttcta
      661 aatctccata atcacatata ttataatcct ctaaatccaa atctaaaaaa ggactatatg
      721 tctcaagacc gtaaaattct tttctcatag agctacttgc aaatctagcc ccaggtctgt
      781 tagaagtagt tccatcaaat ccaacaccaa atactataag attagattct tcataatttt
      841 tgtccatact cataaaagtg ttcatatgat agaaattatt cttcatctag catctccttc
      901 acgtattgag gtaacataaa cgaacctaaa tgtatatcac tattataata tttagttttt
      961 aaacttaatt tctcccatcc atttctatct tgatttttta ctggattaag tttttttgaa
     1021 gcaaatccaa acaaccagtg accagatgga tatgttggta tatgtgcttg ataaacttct
     1081 gatattggaa atatctgttt taatttttta ttagctcttt tcatctcttt agcattaaag
     1141 tcaaaatacg gactttcact ttgatttact aaaattccat catcagtcag tattctataa
     1201 caatccttat aaaaatcagt tgagaaaagc ccttcgcctg gtcctattgg gtcagttgaa
     1261 tctactatta tcaggtcata tgatttatca tgtgcacact taacaaaatt aacaccatct
     1321 tcaaaatata aactcactct tttatcatct aatttacatg agcatatatc catatactcc
     1381 ttagatacat ctacaaccat tttatctatt tcaaccatgt caatcttttc tatttgagga
     1441 taacggctta attctcttac tgtacctcca tcacctccac ctatgactaa tactttcttt
     1501 atgtttaaat ttgtagccat aggaacatga gttatcatct catggtatat aaattcatct
     1561 ttatatgtta ccatcattaa tccatctatt gttagaaatt ttccaaattc atcactatca
     1621 aatacatcta tcctttgaaa ttgagacttt ccttcaaata gatgtttatt tacttttata
     1681 gaaaatctta cattttctgt ccattcttct gtataccaaa gttccatata ataatcctca
     1741 ctttctcttg tatgttttat actgcttctg gtttatgagt aatcttacct aaatcttttt
     1801 ttgaatattt tttaattttt gtagttaagc ctcttggaat ttctgtagac tcgcttcttt
     1861 cagactttaa aagttgctct aataattcaa aacttttcca tgggtctata tcaccacaag
     1921 taaaaaaatc tgctgctgca taaccatatt caggccatgt atgtattgtc aaatgagatt
     1981 cagcaattat aacagctcct gaaacccccc aaggattaaa atggtggaat acagaatcca
     2041 caattgtcgc ttttgcattt aatgctgaat cattcataaa attttctata agacgtgggt
     2101 ctttaagtac gttttcatca caattatagt attctactag tatatgtctt cctagtgttt
     2161 caaatttcat ttcaacccca actcctttat acttttaata agttctattt gataaactct
     2221 acttaacaaa tacttgccct tgcaaaataa agtaatttat tttattacta gtattctatc
     2281 taagttttta tcttgtacat ctgtaagata cgcattgcta ttttttaaga aaataatata
     2341 ctccataatt tcttctgtta tcaattctcc tggagcaata atcggtatac ctggtggata
     2401 tgccattatt gattctccac aaattctatc tatacatgaa ttgatttcta cactttcctt
     2461 tggtgcataa aaagcttctc ttggattaag tttaataatt ggatttattt gaaccatatt
     2521 aaattcattt aatgttgatt cttttctata aatctttgca attatagaaa gtgcctttac
     2581 taatctatct atatctgatt tatttgttcc aatggacaca agtgccagta tattatatga
     2641 atctcccaat tctacttgaa ttgaaaaatt tttatataat aaatcgtata cttcaaatcc
     2701 agtcaaattt aattctttta catttataca caattttgtt tcatctataa atttaacacc
     2761 tttttgtttc aatatttctg tagataaaac ttttattcca tcaattttgt ttaatttaga
     2821 ttttgcatac ctagatagat ttaatgcttt tgacagttgc ttttcaccat tctcaactag
     2881 attaactcta gcaccatcta tacttgccat aagcaaataa gatgctgatg ttgattgtag
     2941 catattgatt gattgaagaa ccttttcaaa acttacatta tctctattca ataagagtgc
     3001 tgaagattgt gtcaatgctc ctccagtttt atgtatactt acagctacca tatcagctcc
     3061 caaacttatt gcagaaggag gtaaatctaa atgaaatgga aaatgtgcac catgagcttc
     3121 atcaactaac actaaaacat tatttttatg gcatagttct ataatactct caaggtctgc
     3181 acaaactcca tagtaagttg gattaaggaa aaataatgct tttatattac aatctttttc
     3241 tatctctgtt ctaacttttt caagagtaat atttaaactt atacctagtt tttcatcaaa
     3301 ttctggttgt ataaatattg gtattcctcc acaaagtatt aacgcattta ttactgattt
     3361 atgtatgttt cttggaagta atactttttc acctggtttt ataacactca aaatcattgc
     3421 atgtattgct tgagttgtgc cattcgttat aaagaatgcc tcatcagcca tatatgcttg
     3481 tgctaacaaa tcttgtgcat tttttataat cccatttggc gcagatacat tatccatagt
     3541 tggtgatgaa tttatatcca ttttcatgag actttcacct agatatttat ttaatatagc
     3601 tacacctcta ttttttacat gtccaggcac atcaaaacaa gctatatctt tctctgagta
     3661 ttcctttaac gactctaata atggagcaca ctcatagctt aattcaacta catttgcaaa
     3721 ctcgctatcc atgatttcaa attcatctcc tctctatatt caaatatttt taatatttat
     3781 tatttattat ttctattttt tatttttact tttattaaac tcatgatatt tattatatta
     3841 acatctcagc atttttacaa gtgttttttt gaagttactc aaaaacatat acattagcta
     3901 tatcaatatt tataaacttt ttaatttttt gttgtttata ttaatttttt taaaacttag
     3961 tatttataaa ctttcttatt ttttattatt ttggtttttt tacttttttg agttttagta
     4021 agcttttgag tttactattt ttaatttata agtattttat tactaaactt ttatattatt
     4081 cttatttcac atattaaaat tcttggttta gttggactaa ataattttcc tatttttgta
     4141 ttttaatttt aatataaaat ttattaaatt ttttaattga taaaataaat tctatattta
     4201 tgatattaaa attctataag ttcattgatt tatatatgtt aatttaaata cattattcta
     4261 tcgtagtgcc ttagagtttt tatttgtatt taaagttaaa tttattcttg tttttccttt
     4321 aatatttaat agtaaaacaa caaaattata aaaaaaataa agagaaactt tataaaagct
     4381 tctctttttt catctcttcc ataggtatcg ttttccaatg ttctctcaaa actctttcaa
     4441 cattttctaa atcattctcc ctcatagatt ttactacttc aatatgttga ttattgctgt
     4501 atgtcaacat tttatataac tcatcttcat tatcactagt atatgacttt gacataaaat
     4561 tatatcttaa agagttcaat agatttacta acacctgatt atctgattta gttatgtata
     4621 tatcgtgaaa ttcattactt aattttaaat agctattgta atttttatac tttatagaga
     4681 ccgccatcat ttccgttaat tcttccattt ttactaggtc tgattcagtt aaattttgta
     4741 atgctgaaga accggctaac gcatccaata ctccaattac ggaataggtg tctaatttct
     4801 ccttttcctg aaattctttt actacaaatc ccttattagg tattttttct agtaaatttt
     4861 cacctgctaa cctagttaat gcttctctcg ctggtgttct actaactccg atagatttac
     4921 aaatctcaga ttcagtaatt ctctgattag cttcatattc tccattttgg attttctttg
     4981 atatatagtc atatacttga gttgtaagtg gatctacctt cataagacgt tcctccatat
     5041 taagattata aaagttaaat ctccctacaa ctttggtaga gagttcagtt ttatcttact
     5101 aatttatata atat
//
LOCUS       DAKBNN010000237         5073 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000237 DAKBNN010000000
VERSION     DAKBNN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.243"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(77..1369)
                     /gene="brnQ"
                     /locus_tag="OUB02_003470"
     CDS             complement(77..1369)
                     /gene="brnQ"
                     /locus_tag="OUB02_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCT8887197.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNNNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
     gene            complement(2488..3780)
                     /gene="brnQ"
                     /locus_tag="OUB02_003471"
     CDS             complement(2488..3780)
                     /gene="brnQ"
                     /locus_tag="OUB02_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCT8887198.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
     gene            complement(4169..5065)
                     /gene="ylqF"
                     /locus_tag="OUB02_003472"
     CDS             complement(4169..5065)
                     /gene="ylqF"
                     /locus_tag="OUB02_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCT8887199.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         1681 a          874 c          562 g         1956 t
ORIGIN      
        1 taattctttt aaatataaaa aggaattgcc tatataattt aaaggcaatt ccataattat
       61 caattagttt ttattattat gcagttttac tgcttttaga tggaataaag tttcctatta
      121 ttcctgctat tacaactgga actatccagt taaatcctaa tgaagcaaat ggtaagctat
      181 taactagagg aatttttaca ccaaaacttg ttgcaactgt taatacactt actaataatg
      241 ccatgtatgc agcacatttg aacacgttgt tatttttaat tttattacta aataatgcta
      301 gtataactag agttatagtt gctggatata ccatacttag tataggagaa gagaattgta
      361 ttattgtact tactccaaag tttgatatga ttgcactaaa tacacaaact actataacta
      421 tatgctcata ctttaatttt ccatctgtaa gtgttgagaa atattgacca gctgctgaag
      481 aaagacctat agctgttgtt aaacatgcta atgcaactat aatagccaat aaaattttac
      541 ctgtagttcc aagtaaagct gctgttattg caactattaa agctgtttgt tgaacatctt
      601 gtccatattg ttgagacaca gttgctccaa gataacataa tccaccataa actaatgcca
      661 ttcctattac tgcaattaca cctgcactta ttgtggcttt catcttttct ttctcacctg
      721 tataaccttt agctattact gacatgatta taattgatgc aaatacacta gctgccaatg
      781 catccattgt ttgataacct tgagttatac cttctgcaaa tacattttgt attaaagaac
      841 tgtctcttat ttctcctaat ggagatacga tacctttaac tattaatact gccaaagcta
      901 taagtaaagc tggagttaat attgacccaa ctatatctac aacttttgat ggtcttattg
      961 ttaaaaccaa tactagagca aagaatacta tagagaatat tactgggctt attccagaac
     1021 ctattgtagg taatataccc atttcataag ttgttgctgc tgttctaggt attgctaaaa
     1081 gtggccctat acagattacc attgcacatc ctaatgcaat acttaatcct ttgcctgctc
     1141 ttgagaacat cttcgatgtg tctccattga acttagttgc tgctataact gctaataatg
     1201 ccaaaccacc atctgcaaaa aggaatccta tgaaagctac tagccaacta cttccagata
     1261 aaactcctag atatggaggg aagattaagt tccctgcacc aaaaaacatt gcaaatagtg
     1321 caaatccaat tacaattaca tctttaaaat tgatttgttt ctcacccatt taaagcaccc
     1381 ccatttgaat tttactacct tttactaaaa aataagtcca atctcttaat taaatgaaaa
     1441 ccttttgacg agattttatt taattataca tttttgactt aaatttgtca attatgtttc
     1501 ataatttata aaatcttcac aatatttaat acaactttct taattaaaca aaatcaacct
     1561 gtatacttta aacatagttg gttttttgtt ttttctctaa tttttcttga taaaattaac
     1621 tattccgaat aattaaaata ttaagacttt ttagattatt cgatttatta ttaagttgta
     1681 ttgctttata tactgtatat atcactctcc ttttttacat tcatataatc tttttaattc
     1741 aatttaaatt ttaaatattt tgtgtttatt atataatctg aaaaaaaagc agtcaatagc
     1801 atttttaatt tttttaattt tcaaaattat gattcttaaa aaatcgtttt ccttttatca
     1861 taacacctat ttacgttaat tcttgtagta ttttgtattt attttgctaa attgacagtt
     1921 aactttattc aatttttatt ctacttgcat agatttgcct cttgtttttt actcttaaaa
     1981 ccattttttc tgaaaattcc ttattatgat ttatcaactt atttttcttc ataacaatta
     2041 ctctctaact tccaacttac ttaaactttg cagttgctag tattcacgtt tacatactta
     2101 tatgtcataa ttaactaaaa attcttaata tatctctcaa tgaaaacaat ttactcattt
     2161 atttaataca tttatataat catatgtctt aaattttgtt ttattatttt attattgtca
     2221 caattttatt tatattatat ttttgtaaaa atcatttaat atgtatactt aaatttataa
     2281 aattactcta aaattctttt ttaatctcta ttattgttaa aaaataaacc atatattttt
     2341 ttgatatttt cttatacttt ttccatatta aatacaaata attaaatata gaatctaaaa
     2401 ataaaaggag ttatttgtac aaataactcc ttttactttt agttaactca tttatttaca
     2461 attgattata ttttaattaa acaaaactta gtttgttcca agtgtattag attgactctt
     2521 tgatggtata aaattaccaa ttattccagc tattataact ggcaccaccc agttaaatcc
     2581 taatgaatta agaggtaaag aatttactac tggaacagct actccataac tagatataac
     2641 tgttagtata cttacaagca atgacatata tactgctcct ttgtatacat tatcattctt
     2701 aattttttca ttgaagaaag ctaaaattat taaaacaatt gatggtggat aaactatact
     2761 tagtatagga gatgctatct ttattatagt tccaacacca aaagatgcta ctactgcact
     2821 gaatacagat acagctaata ctattttttc ataactcaat ttaccttttg taagtctaga
     2881 gaaatattga cctgtggcag atgtaagacc tatagatgta gttaaacatg ctaatgatac
     2941 tactactgct aaaatcgctt tcccaacatt tccaagcaat ccttcagtta tatttactat
     3001 tacttgtgat tgtactgcat ctgttccata cattgttgat acagttgccc caaggaaaca
     3061 taatcctcca taaactagag ctaatcctcc acaagcaaga acacccgcct taccaatcat
     3121 tttcaacttg tcactaatag tagaatatcc tttatcattt aaagatacta ttagcactga
     3181 tgcaagtgct attgctgcaa aagcatccat tgtctgatat ccttgaccta taccttctgc
     3241 aaatacatta ggtatcatag gtttatctac tatatctcca agaggtgata taataccttt
     3301 tataattata aatgctaaag ctattagaag agctggagtt aaaaactgtc caactatatc
     3361 tacgacctta gaaggtctta ttgttagtac atatgaaatc gcaaaaaaaa gtatacagaa
     3421 taataacact ggaattgaag ttccaaacaa aggcattata cccatttcat aagttgttgc
     3481 acctgttctt ggtatagcta agaatggtcc tatacataaa atatctgcac aacctataag
     3541 aattcctaac ttcataccac atcttttaaa catttcaact acgtttccat cgaatttagc
     3601 tgtagccatt actgctagaa gtgccaatcc agcatcagca aacgtaaatc ctaagaaggc
     3661 aattaaccat tctggacctg taattattcc caagtaaggt ggaaatatta agttacctgc
     3721 tccaaagaac attgcaaata acgcaaatcc aaatactata acatccttgg tcttacccat
     3781 tataaaatcc ccctttttaa ccttattaaa ttaataaaat gtctcgattg tttacgcttg
     3841 cataagtaag ttgaattaaa caataagaac ttattattaa attaataaaa ttaaataaat
     3901 taaattttta atatattgta tttatttgtg cttattatat attatacgaa acaagcaagt
     3961 caatatgcta aaattttaaa taatcacact cattttaatt ttcaaaatat taggtatatt
     4021 tgtcttaaat taatattttt aaaggttttc taacctaatt tatactttta tatggagata
     4081 tttgtctata tttcaaaaaa ataaaaaagc ctttgctaaa aggctttttt aaatttttaa
     4141 cattaaattt ttgattttgt tttaaaatct atcttttaac gttttctgga acttccaatg
     4201 ttatatttcc tatttttcca tctctaaatt catttaatac agttgtggct atgcgtgtat
     4261 aatctagttc ttttttacct gttataaacc ctcttttatg gccaatcata tccatagttt
     4321 caattggagt ttctcctaga caatctaact tatatctggc tttcaacttc tcaggttcta
     4381 ttttcataag tttctctata agtcttaaag ccaaagtttc agtatctaaa atctcatcct
     4441 ttatagctct actaaatgct aaatttaatg caacttcttg gtcttcaaac ttaggccaaa
     4501 gtattccagg tgtatctaaa agttcaagat ttccctttaa tctaacccat tgtttccctt
     4561 tcgttactcc tggcttatct ccagtttgag tgctttttct acctgttaat ttgtttataa
     4621 gagacgattt accaacgttt ggaactccaa ctatcattat tcgtatagct ctttcttttc
     4681 tccctttgtc ttttaaagaa ctcatttttt ctctagttac atttttacat tcttcgatta
     4741 tcttatttat accaacgcct tttattgtat ctactggtat agctttgata ttaattttct
     4801 tatagtattc aatccattga ttcaatttat ccctatctgc catatcactc ttattaagta
     4861 cgacaactct tggcttatct cctacaagcc tgtctatgtc tggattttta ctactaaacg
     4921 ggattctagc atccaaaagt tcaattacta catcaatcaa ttttaagtta ttttttacta
     4981 actccttagt ttttttcata tgacctggat accagtttat atgtagattg tcattcataa
     5041 gatactcttc gtattcattt gacacatgta tca
//
LOCUS       DAKBNN010000238         5051 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000238 DAKBNN010000000
VERSION     DAKBNN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.244"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(67..279)
                     /locus_tag="OUB02_003473"
     CDS             complement(67..279)
                     /locus_tag="OUB02_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887200.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(280..696)
                     /locus_tag="OUB02_003474"
     CDS             complement(280..696)
                     /locus_tag="OUB02_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887201.1"
                     /translation="MSKSETGVRSKLILELIVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(764..1177)
                     /locus_tag="OUB02_003475"
     CDS             complement(764..1177)
                     /locus_tag="OUB02_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887202.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLISVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(2011..2640)
                     /locus_tag="OUB02_003476"
     CDS             complement(2011..2640)
                     /locus_tag="OUB02_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCT8887203.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLRVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2719..3681)
                     /locus_tag="OUB02_003477"
     CDS             complement(2719..3681)
                     /locus_tag="OUB02_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCT8887204.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(4443..>5051)
                     /locus_tag="OUB02_003478"
     CDS             complement(4443..>5051)
                     /locus_tag="OUB02_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887205.1"
                     /translation="DSGNGGNSKPDSGSGGNSKPDPDNGGNSKPDPDNGGNGKPDPDN
                     GGNSKPDPDNGGNSKPDPDNGGNSKPDIDNGGNSKPDIDTDGDGKPDINIDTDGDGKP
                     DINIDTDGDGKPDINIDTDGDGKPDVNIDIDGDGKPDVNIDIDGDGKPDINIDTDESL
                     DSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         1728 a          816 c          501 g         2006 t
ORIGIN      
        1 taataaactt aataattaaa taactatatt tcattatcca tcttataaat gtttataaac
       61 tttccactat tcttcttcaa taaattcaaa caaattttct actgtagtat cgaatacttt
      121 agcgatatcc atagctaact tcaaagaagg attatatttc ccattttcta agtgtacaat
      181 agtctcacgt cttaccccaa ccaatttagc aagttcattt tgcgtcataa ggagtttttc
      241 tctgtattct ttaatttttg ttttcatcaa tattgccact tagttacata tccccctttt
      301 atcaaagtat atatacaaca ctaatttaga taaagactgt ataaataaaa ctgcaatgag
      361 gattatacct gtatctaggt ttgatatatt tatattactt gtataaggtg ttgcaaagaa
      421 aatagatatt attactatag aatatatcat tatttttatt gcaatcttat taacctttga
      481 tagtataaat tcagatgatt catcaactaa atcattatat tttttattat aaattgaata
      541 acatccaaaa actattaacc aactcaaccc tggaagagaa gcagtgcttg gacctttaaa
      601 aatatactta gtggctaata cagctataaa aaaataagtt attacaatca actccaggat
      661 aagctttgat cttacaccag tttctgattt agacatataa aatcactcct taaaaattat
      721 attctctata ctctttaatt ttcgttttta tcaatattat cacttagttg catattccct
      781 ttctatcaaa atgtatatac aaaattaatt tagataaaga ttgtataaat aaaatagcta
      841 agagaattac acctatatcc aaatttgata tatccaaacc tcttgtataa tttgctgaaa
      901 agaaaacaga tattagcaaa atagaatata acattatttt tgatgcaata tgatcaacct
      961 ttaataacat aagttttgac gcctcgtcaa ctaaatcatt atatttttta ttatacatga
     1021 agtcacatgc ccaaaaagcg aataaaatta taaaagtaat acaaagattt ccctctttaa
     1081 aaataaactt ttttaataat gagtctataa aaaaatagga tatcataagt aactctgaaa
     1141 caagatttgt tttaacacca agctctgatt tagacatata aaatcacccc ttaaaagtta
     1201 tatatttata acaccttatg ttataaatat ataacttttc caaataaatt gcaatagaat
     1261 attgaaaata ttcaataata tcttcataaa tatattttat aaaagatttt ataataaaca
     1321 ttacaataca gtactaaatt agtttatttc atctatcatc tcaatcatat catttactaa
     1381 ctattattct aatgagcata taacattgaa acttataatt atacaaccta aatgcctccc
     1441 aatgtaccct caaaaacatc atttcctgta actgaagtat aattggtata ttttatcact
     1501 gaaataaaaa agcactagtc aataaagact agtattttca agtattttat cattcttttt
     1561 ttaattattg gagacgaatc atcagaataa tgcccataat ttatctatat gcaaattcta
     1621 aatttaaaat aaagtttcag ataaatttac tattctatgc tatgatacaa cagaaaaaac
     1681 tgaaatctat gagtggtagc ttcatataat cctagttcct acagtaatca aaactgaggt
     1741 ttattctata tgtaactaat gataaatttt attataggag ttattagctc tatttgtgtt
     1801 actataagta gtaattatat atatgataat tttttaacac ttttctgttg gctatataga
     1861 aagtggttta gaatttaaat ttaaataaaa tttaaattca ccaaaaagaa atctatatta
     1921 gaaaccacta ctctattcca ccagattgta attttttgtt ctttatatca cagtgctata
     1981 ccatattaaa ttgaaaaata agttttaaat ttatttgtta caattcatta attcaaaaaa
     2041 atattttgag tcatgagttt tgtaaagatt tagccatgaa tttaatgctt ctgattcaaa
     2101 aacatctaaa agtttcaata cttgaaacgt aaattccaac ggagcaattc ctgaagccgt
     2161 aactaaattt tcatcactaa ctacagattc cgttttataa tatttttctc cagagtaatt
     2221 tggtattacc attttaagaa actctaagtc attacttgta tgatttctaa aatctaatag
     2281 ccctttttta gctaggccta atgtagctcc acatatggct gcaactatag aatcttgtat
     2341 taaagcttct ttaacttttt ctaatattat ctcatgattc atggataacc aagtatttcc
     2401 ccctggcaaa attaataaat ccttactttc catattaaac tcatcaactg taatttcagg
     2461 taaaactctt aacccaccca ttgttataat aggattttta tcaattccta cagtaattac
     2521 ttttagtggt tttaatcctt ttttaaaata tcttcctgta tttaattcag cagttaaata
     2581 tgccacttcc caatctgaca tagtatcaaa tacatacata tatacttttc tatttttcat
     2641 cttttttact ccttatgtaa ttaatttata tatctaatta taaaataact taactgacat
     2701 attcagtcag ttaagctatt aaatttgata ataatcaatt aattctagta aatcttcaat
     2761 tattttcttc ctaaggctta gtggttcaat tacttgtatt gtctttctat aaggtaaaag
     2821 tatatttggt atgtacttgc atattacttc ctcttcaagc ataaatatta ttttattagg
     2881 tgttctttct ttcaagtagt gccctaaaaa ccaatgttgg cttaaactat ctaaagaatc
     2941 tatactccct tctacaacta attgaattat attttgttca tcttttattt caggtataat
     3001 tccttttata aaaaattcac ttgctgaaaa ttctgtcggt ctgttaaatg tatttgatgt
     3061 ttctatgatt tcactaatcc tatccactct gaaactacgt acctcatttc tcatattaca
     3121 aaatgcaact acgtaccaat tattattcca gtgaacaatt ccatatggat ttataattct
     3181 atattttgat tttgctttac gacctgcatg atagttgatt tctacagata gctcctttaa
     3241 tatactattc tccaattttt taagtactga ttctatagac actttcccaa ttggttttat
     3301 tacttcaagg gctcttaagt ggtttgttaa aatatcttct tgtttttgat ttgaatacat
     3361 ttttaacttt aatatcgatt tatctaatgc ttcgtctaag aagtatcctt cttttttagc
     3421 aaatagtgct gcatgaagta gagatttttg ctcttctata tcaaaaaaaa gaggtgcttt
     3481 agtaaaatta tttaaaaggc tatacccacc attatgacct gtatctgaaa ttataggtac
     3541 tccactcgca gaaagtacat caatatatcg gtacactgtt cttatattta tttctaattt
     3601 ttcagctatc tgttttgctg taattttttt gtcagagttt aacatccaca gtatagacat
     3661 catattgtca attttagcca tgtagtttca cctctttcta tttgttatat aaagttatta
     3721 tataactttt tgattgattt tttacaaaaa aacattagta aactgctaat aatttatact
     3781 aagatttatt tcaaatattt atagatagtc tttttctata gttaagttat ccattaaatt
     3841 acatagtagt atatccattt tagttgatat attttcagct acctcccttg aatattatta
     3901 caataagtct aaatcttctt tcttccataa atatattatg aggaaagact atcatcttta
     3961 atggtttaat agtataaaag atattaattt taaaaaatct atttttatat cagatgggta
     4021 ttgtttttta taaaactgaa aaaatcacct aatagaatct atgatttttt aacataacct
     4081 ccataaatta gtaccaatta tcacttcaaa ttcaaatttt tatctgaaga atatacaatc
     4141 atatttccat actcaacata atattgagtg tttggcttgt atttacaaat tattattcta
     4201 acaaaaacaa ctatacatag attaaaatca tcttctaaac ataactacta aaattgaatt
     4261 aatgatatca attccattaa tctcttttaa taaaaaattc tcttttctat atgaaaatta
     4321 tttcaaagct tttaagttaa atacaaaaat gactataaat agatttttat ttacacaaat
     4381 ctatttatag tcactatagt ttatccaata aaataataaa ataaatatgt acacaataaa
     4441 tgttatttac gtcttaagta tttatttgtt agatttttaa ttaaaaaaaa caagctaatg
     4501 aacattaatg ctaaatctag cattatattg ctttgatctc ctgtttgtac attttgacct
     4561 acattactat ctaaactttc atctgtatct atatttatat ccggttttcc atctccatct
     4621 atatctatat ttacatccgg ctttccatct ccatctatat ctatatttac atccggcttt
     4681 ccatctccat ctgtatctat atttatatct ggttttccat ctccatctgt atctatattt
     4741 atatctggtt ttccatctcc atctgtatct atatttatat ctggttttcc atccccatct
     4801 gtatctatat ctggtttgct atttcctcca ttatctatat ctggtttgct atttcctcca
     4861 ttatctgggt ctggtttact atttccaccg ttatctggat ccggtttgct atttcctcca
     4921 ttatctggat ctggtttacc attgccaccg ttatctggat ctggtttgct atttcctccg
     4981 ttatctggat ccggtttgct atttcctcca ctacctgaat ccggtttgct atttcctcca
     5041 ttacctgaat c
//
LOCUS       DAKBNN010000239         5013 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000239 DAKBNN010000000
VERSION     DAKBNN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.245"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(517..2652)
                     /gene="rnr"
                     /locus_tag="OUB02_003479"
     CDS             complement(517..2652)
                     /gene="rnr"
                     /locus_tag="OUB02_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCT8887206.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3248..3862
                     /locus_tag="OUB02_003480"
     CDS             3248..3862
                     /locus_tag="OUB02_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCT8887207.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3880..4242
                     /locus_tag="OUB02_003481"
     CDS             3880..4242
                     /locus_tag="OUB02_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887208.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4348..4845
                     /locus_tag="OUB02_003482"
     CDS             4348..4845
                     /locus_tag="OUB02_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCT8887209.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1630 a          756 c          608 g         2019 t
ORIGIN      
        1 aaattatatt ttttaatatc tattcttaat taggtatttt taaattatct gtttacatta
       61 aatatttctt aattacttat ctttcagcta gctttttcta ttaaatgttt ctcaattact
      121 tatttttcaa ttagccactt ctattaagca tttcttaatt acttattttt caattagtca
      181 cttctattaa acatttctta attatttatt tttcaattag ccacttctat taaacatttc
      241 ttaattattt atttttcagt tggctttttc tattaaatgt ttcttaatta cttatctttc
      301 agttagctct ttctattaaa catttctcaa ttacttattt ttcaattagc tactttcatt
      361 aaatatttct taattattta tctttcagtt aactatttcc attagatatt tctcaattac
      421 ctatttttat catttatttt atagagtatt gtgcactata tatttataca tatcataaat
      481 actacaactt gttatctctt attttagctg ttttatctac tcctctattt tttcaactat
      541 ctcaaagtca atttctttaa aatctacatt aacactttca acctttatct taacaaggtc
      601 tcctatctta tatgacttct tagttctttc tcctaaaaca ctataagaac tctcatcata
      661 aatatagtaa tcatcattca tatttgcaag tcttatcaac ccttctatag tattatctaa
      721 ctcaacaaat attccaaatg aagttacgct agatacaaca ccctcaaatt cttgaccaac
      781 tttatccttc atataaagag ccttatagaa atcatgtaca tctctttcag ctaactctgc
      841 ctttctttct ttctcggatg attgaacaga tgcataatcc actatagtag taagctgttc
      901 ttgtcttttt ccacttattt tattatttaa agattctttt attattctat gaatttgaag
      961 gtcaggatat cttcttattg gtgaagtaaa gtgacagtag aatttagctg ctaatccaaa
     1021 atgtccacta cattctggtg agtacttagc ttgttttaaa gaacgaagca ttatagtact
     1081 gatagctcct tcttcttttt tgccctttat tttctctatt ataccttgta atgcttttgg
     1141 atgtacttcc tctttatcac cttttattat atatccaaat gtagatataa acttactcaa
     1201 ttcttccatt ttttccaaag atggtgtctc atgtattcta tacacaaacg gtattcccat
     1261 ccagtaaaaa tgttcggcaa ttgtctcatt acttataagc ataaattctt ctattatctt
     1321 attagatatt cttctttcat atggctttat atctacaact tcgccttttc catttagaat
     1381 tatctttgcc tctgggaaat tgaagtctat agctcctctt ttttcccttc ttgacataag
     1441 aatcctagct aaaatttcag catttttaaa atcttcaaca acatgttcaa aagtcttttt
     1501 aagtttttca tcatcctttt ctaatatatc agatacttct gtataagtca ttctagcttt
     1561 tgaatttatt acagattcac atatttcatg tttaaccaca tttcctttat ggtctatttc
     1621 cataaatata gaaagagtta acttatcttc aaatggattt aagctacata ctccatttga
     1681 aagagttttt ggcaacattg gaattacttt atctactaaa taaactgatg tagctctttt
     1741 taaggcttcc ttgtcaagtt tatttttctc tcttacataa tgtgtaacat cagctatgtg
     1801 aactcctagt ttgaagtttc cattaggtaa tacttctatt gatatggcat catctaagtc
     1861 ttttgcgtca tctccatcta tagtaaatat gtttaaatct cttaaatctc ttcttctttt
     1921 tatctcttct tgaggaatct caacagctac agcttctgct tcttgcaata ctttctttgg
     1981 aaattcttct ggaagaccat gttctcttat tatagaatct atttctacgc ctctttcacc
     2041 tttttgacct agtatctcta taattttgcc ttctggtttt ctatcctctt gtggccatac
     2101 tgtaatttca catactacct tatcattatc tactgctcca ctaaaatgtt ttttaggtat
     2161 atatatatct ttagtaaatt tcttgttgtc tggtgttaca aatccaaaag ttttacttga
     2221 ttggaaggta cctactattc tagttatttc tctctttatt acttttacta ttcttccttc
     2281 tgctctcttt tcttcagttg caggtactac tatttctgcc ataactctat cattatgcat
     2341 agcaccattt atattgtcac ttgagataaa taaatcctgt gtatattcaa catcagattc
     2401 tacaaatcca aaaccctttt tgtgtgatac aaattttcca acaaagtatc ccatttgatt
     2461 gggagatact atttttcctt ttttagtaaa gcagatataa ccatcttctt ctaattcatt
     2521 taaaaaatta taaaacattg gcatttctga tgaatgtata tcaaatattt ctgctaattc
     2581 ttcttttttt agaggattat atgcactttc atttattaat cctagcaaac tttctttaag
     2641 tcctggtatc atttttccct cctgttttac ttttcattat attattattt atattgtctt
     2701 ttatatttct tcagacaaaa cattttttac ttatcacata attttctata tttccttatt
     2761 attattacaa gttaaaatta cttatttata tttttgattt aatgtaagct ttgtcatact
     2821 aacttcaaac aaattatgat gttttataaa ttatttgtaa atatcattat aattacttaa
     2881 gttattcttt atcataattg cttaaattac tctttatcac ctaattatac accatcaaat
     2941 aggattggta aaatattcat ctcatataag tattactata taataattta ttctattagt
     3001 atactcaaat catgtaaaat ttatataact caattaattt tttaaataca actttataat
     3061 ataaaactta taataaaagt aaatttatat gttaaatgag gttgattttt tattattttt
     3121 tatttatgct agtactaaag acttgcaaac gttttttgac aagtctttat tttgtctaaa
     3181 ttaaacaaac ttttttacaa ataaatacat aaaatttaaa ctaatctatt tattggagga
     3241 gataaaattg gaagaagtaa ttgcaaaaat tattactata gaaaatggat tcaaagaaat
     3301 tgaaaacatt gctaaaaaaa ttgtaaaaaa taatgactca aaaaagtgtt attatctatc
     3361 aatctactta tataggtcag aatattatca agtaagagaa ttggctgtgt ttatacttgg
     3421 ttttatatca gtaactattt ctgaagcttt gttattttta aaagataatg tgagtaaaga
     3481 tgaaaactgg agagtacaag aaatactagc taaatcattt gattattatt gcagtgaagt
     3541 aggatatgaa aaggctcttc ctgttataaa agaatggctt aattctgata gtccaaacat
     3601 aaaaagggct gtgacagaag gtcttcgaat ttggactagt agagactatt ttaaatctaa
     3661 tcctgacgtt gctatatcac tactttctag tttaaaagaa gatgatagcg aatacttgag
     3721 aaagtctgta ggtaatgctc ttagagatat aagtaaaaaa tatccagatt tagtaaaaaa
     3781 agaattagat tcatgggata tatcaaataa aaaagttgag tttgtatata agcttgcttc
     3841 aaaatgtatc ttggcaaagt aaaatggagg tgaacttatt tgaaacttcg tgatgagtat
     3901 acttgcccat tagaactaac acatgatatg ataaaaggca aatggaaatc tattatatta
     3961 tggagattac gtataggacc aacttcctta tcaaaacttg aaaaggaaat caatggtatt
     4021 aatcaaaaga tgttacttga acaacttaaa gaattaattg aatttggtct tgtagataaa
     4081 aagacatatg aaggttaccc acttaaagtt gaatactcac ttacacctat tcgagggaaa
     4141 caaatattgg atgctttggc tataatgcaa gatataggaa ttagctttat gttggataat
     4201 ggtatggaag atattcttaa ggaaaaaggt gttttaaagt aaatagatac ccacaaaaaa
     4261 gtaagtaatt taccattatt ttcaactggt atataattaa attatagata tattctatta
     4321 atttattata ataggaggta aattattatg aaagtaacta gtacaggtat agtaaatggt
     4381 gttatagaag ataaatacgg taaaagagga actcagttca atgaaggtgg tatgcctaca
     4441 tattcactac ctctaaagtt tgaagatgct ccaaagaata cagtatcctt tgctgtattt
     4501 ttagaagata aagactctgt tcctgtatgt gggtttgcat ggatacattg gttagctgta
     4561 aatataacaa aagatgagtt aaatgaaaat gaaagtgtaa cagctactga ttttgtacaa
     4621 gggactacta gttggcatgg taaactagga ggtcttgaca gattagaggc cagtgtatat
     4681 ggtggaatgg ctcctccaga cagacctcat gtctatgaaa tacatgtatt tgcacttgat
     4741 actatccttg accttgaaaa aggattctat atgaatgaat ttttcaaagc tattgaaggg
     4801 catgttttag atacatttac actaaaagga agttattcta attaattagg ttgactatag
     4861 ctaatcatta attaaatgct acatttaatt gatgattagc ttaattgtta ttagaatatt
     4921 ttctgcactt tatcttaata tatattttag ttgactggta ttttaatttc tattagatta
     4981 ttatcaattt atataaattg attttaactt aaa
//
LOCUS       DAKBNN010000240         4986 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000240 DAKBNN010000000
VERSION     DAKBNN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.246"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(42..1241)
                     /locus_tag="OUB02_003483"
     CDS             complement(42..1241)
                     /locus_tag="OUB02_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HCT8887210.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            1360..2247
                     /locus_tag="OUB02_003484"
     CDS             1360..2247
                     /locus_tag="OUB02_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCT8887211.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGNT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            2437..2994
                     /locus_tag="OUB02_003485"
     CDS             2437..2994
                     /locus_tag="OUB02_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887212.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(3233..4093)
                     /locus_tag="OUB02_003486"
     CDS             complement(3233..4093)
                     /locus_tag="OUB02_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCT8887213.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            4587..>4986
                     /locus_tag="OUB02_003487"
     CDS             4587..>4986
                     /locus_tag="OUB02_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8887214.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLKKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIK"
BASE COUNT         1848 a          691 c          637 g         1810 t
ORIGIN      
        1 aatatatata atccaaaaaa tttaaatctt attaaaacat actattttgt atagttatcg
       61 aaatatcctt gtatgtaaat aataggagta cccttatctc cacttccaga tgttaaatct
      121 gctaaagaac ctataaggtc tgttaatctt cttggagtag taccttgaga aaccatgttt
      181 cctgttaaat catcagattt attatctttt tcaactatgt attttgaaat tgcttctttt
      241 aactcatcac cagataagtc agcaaaatca ttatctgcta agtattttaa ttttacttcg
      301 tttggagttc cctctagccc ttttgtataa gcaggagata ctactgggtc agctaattcc
      361 catattttac ctacagggtc tttaaaagct ccatctccat aaaccattac ttctacatct
      421 ttccctgtta tttcttttat attaccttgg attttgttta caacttcatc acaatttata
      481 gggaataatt ttactgtttc ttctgtagct ttgtttgaac ctaaaagtcc atactggtca
      541 ttgtaaccac taccatctac acttgctgtc attatatcat caagtgaata tactttttta
      601 gctccatttt gtcttaaaat tctctttgtt ctttgtcttg tatgtatatc acaagttaaa
      661 atacttgttg tatagttaag aatagttttt ggattgtttg caaatactat ttctacctct
      721 gcaccacaat ccttgattaa attcttataa taatcaacat aatctacacc agtaaatgta
      781 tgcttgttat atccaaatag ttctctgtat ttttcttctg ttaaaacatc tgaccaagga
      841 tttattccct tatcatcaag ttcatcaata cttattaagt gatttccaac ttcatcagaa
      901 gggtagctta acattaatac gacttttcta catccttttg caattccttt taaacatata
      961 gcaaaacgat ttctacttaa aattgggaat ataaccccaa cagtatcatc tccaaattta
     1021 tcttttacat cttttgcaat attgtcaacg tgagcataat ttccctgtgc tctagctaca
     1081 acagcttctg taacggcaat tacatctttg tccctaactt caaagttttc actttttgac
     1141 gcatttaaaa cagaatcaac aactatttta actaagtcat ctccttgtct tataataggt
     1201 gctctaaccc ctcttgaaac agttccaact aatctatcca ttatctaaaa tcctcctaaa
     1261 gaattaatat tgtcatatta gtaccatgta tattataatt caaatataat tataagtaaa
     1321 ataaataata ctaatatttg acataaggag taattatata tgaatataaa acttgaactt
     1381 tataaggttt ttaatgcagt tgtaaataat aagagttttt ctcttgctgc taaggaattg
     1441 tttatgtcac agccagctgt aagccagtca ataaagcaat tagaagaaca attagatact
     1501 ttattatttt atagaaacaa taagggagta aaattaactc cagaggggaa aattctaagt
     1561 gaacatgtca ctactgcatt aaagttaata tcatcaggag aagatagaat aaataaattt
     1621 aaaaaattag aatacggttc tctgaaaata ggagtaggaa atacagctgc acgttttttc
     1681 ttgttaaaat atttagaaat ttttcataag aagtatccac atatacatgt atctacaata
     1741 aatagaacga gtagagaact tatatcttta ctcaaagatg ggaatataga tatagcaatt
     1801 ataaacatgc caatagaaga tgatacacta aatattgttg aatgtattga aatacatgat
     1861 atatttgtat gtgcaaatga ttacatagaa tacaaaggta ggaaaatatc attggaagaa
     1921 ttaaacacat tgcctttaat aatgctagaa aataaagcaa attcaagact ttatgtaaat
     1981 gaatattttt tatcaaaagg tataaaacta aacccggata tagaattggg ttctcatgaa
     2041 ttacttttag aatttgcgta tataaatctt ggagtgtctt gtgttataga agaatttagc
     2101 atagattatt tggaaaatga gaaattgttt aaattagata taaaagagcc aatacctaag
     2161 agaaatatag gttattgcca tttaaaagat atatctttat ccttagcaac caaagaattt
     2221 ttaagcatga tatctaataa tatataattt agaatacata tttcacaagt aatttaatat
     2281 aaaaatagag ataaatttgt aaatttgaaa gattcatata gtaattagtt tttattaaaa
     2341 ataaaaataa ggttaatttg agaacattta tataattaat atcgtatttt aaatatgaac
     2401 agtatattta gattaatgta taaaggggga ttagaaatgt ctgattctga atatcctcaa
     2461 atgaaaacct tagaaactag aagttgtata ctaagacctg catcattaga tgatgttgaa
     2521 gatttttttg actgttataa aaataaaata gtagtaaaac atttaccatt tagtgagcat
     2581 aaatctttag aagatacaag aaagtttata aaatcatttt ttctaaatcc atataaaaaa
     2641 gggaaaattg gccactttgc tatagtatat aaaagagaca ataaagttat tggaaatatg
     2701 gggtttaata atataaatcc taaagcatta gaagcagaaa taggtatatg tataaatcca
     2761 agctattggg gacatgattt tgctactgag ataacaaaaa gagtaataca gtatggattt
     2821 agagaactaa acttaaataa aataattgca ataacctatg aagaaaacca aaattctaca
     2881 aaatcattag atttattagg atttaaatgt acaggaaagt atatcaaaaa aattcatact
     2941 ggaaatactg taaaaaatgt tccttgttat caatatgaat tgaaaaaaca atagatataa
     3001 tcatttaata tctatgctgg ttatttagta atgattgttt tctaaataaa atttcaataa
     3061 agtggttgcc tcaaaatata gataatcctg tttttattgt taaaatactg aaaatgcatt
     3121 tgattttata caaaaaagag tgtctcatga actaaaaagt ttatgagaca ctctttttga
     3181 ataaatcgta ttttaattaa aatagtatat atacttaaaa tattaatttt tattagttct
     3241 ctaaattttt aaatccttct ccaagaacct catgtacatc tgtaacaata acaaatgcag
     3301 tagggtcagt tcttttaact aatctcttta gataaagttc ttgtttttta gaaactacga
     3361 ctaatagaac ttctttattt tctctagtgt atccaccttt accatcaaga atagtaagac
     3421 ctctatcaag ttcttcagtt atagcttgtc ttaaactttc aggctcatca gagataataa
     3481 aaaatgcttt tgaatggtca aatccttcca ttatcatatc tgctattttt attaatacac
     3541 aaagtgctat accagagtaa agagcagttt ctaaactacg atttactata ccagaagata
     3601 taacaaccat tccatctagg catgacatga attttgtagc ttttattcct gggaatttct
     3661 tactaagtat gagtgcaatt aagtctgttc ctccagtaga agcatctgat ttaaacatta
     3721 ttcctaaacc tataccaact aggattcctc ctgcaataga agacaataat aagtcattag
     3781 taaatcttag ttttgataat gggtctgtta attgaactat agctgaaaat acaactgttc
     3841 caaataaagt ttttaatgaa ttttttgtac ccataattct aaaagcaagt attactaatg
     3901 ggacacctat aattaacatg atagctgata ctggaagtcc tgtaaccttg tttagtacta
     3961 atgatagacc actaagtcct ccaggagcaa ttgtatgtgg ttttaaaaac atatttatac
     4021 caatactcat tgatatacaa ccaaaaaata gtccaagcat ttcaagtaaa attggcgact
     4081 tttgtttttt catataaaca cctccatgaa cttgtcacaa tttaatatta taacatattt
     4141 gaccagttta tattatataa aatttcttga tataagcaat ttcaagtatt tcattattct
     4201 taatattttt gaatttttat atagtgtttt catatcttta ctgatgttgt tacactccta
     4261 tgaagttttt tatatattat aattttttta ttcttttata aagtatcccc tataaagatg
     4321 gttctatgaa aattttctct atactgttta acgtatatag agattttgtg tataattata
     4381 aggtatttga tggaaaaatt tatcttactt ttatatgtta tcaaaatgat ttattaaata
     4441 caacagacag cttgataaca tataattata taaatataat ttatgtagat agttgtattt
     4501 ttaaataaat ttttacatat caataattat tgtctatttt atataagcaa caatcaaaat
     4561 aaaagtttaa gaaaagagtt gattaaatgc ataaacctag ttataaaaca gtatttcatt
     4621 catacattat ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct
     4681 tatcacttat tactattcag aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa
     4741 aatttacaga aaactttaat gaacaaatcg tatttataga taataagcca caagtaaaac
     4801 agtctggatt aaaaaaactt cgagaaaatc aattgtggtt gcaaatttta gattctagtg
     4861 gaaatagagt ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg
     4921 aattattagc attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa
     4981 ttaaaa
//
LOCUS       DAKBNN010000241         4981 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000241 DAKBNN010000000
VERSION     DAKBNN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.247"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            276..965
                     /locus_tag="OUB02_003488"
     CDS             276..965
                     /locus_tag="OUB02_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887215.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKSGIALNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGMEKNNYIKT
                     LWGIGYKMEEE"
     gene            965..1882
                     /locus_tag="OUB02_003489"
     CDS             965..1882
                     /locus_tag="OUB02_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8887216.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1955..2881
                     /locus_tag="OUB02_003490"
     CDS             1955..2881
                     /locus_tag="OUB02_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887217.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKLNSGEIVMFGKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2878..3558
                     /locus_tag="OUB02_003491"
     CDS             2878..3558
                     /locus_tag="OUB02_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887218.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTLVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4003..4704
                     /locus_tag="OUB02_003492"
     CDS             4003..4704
                     /locus_tag="OUB02_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCT8887219.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGKHTLDDVQETVNSSYVFDTIIDSLKEKITHNFLRNLHSSLIFNTTLN
                     SRGMSGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT         1911 a          534 c          788 g         1748 t
ORIGIN      
        1 gtataaaata ttttttagca aaagttaaaa ggtgttttat aatataaaat agttaattgt
       61 agatgttaat aaaagaaaat tatgtatatt tcagataaag ataagagagt atactatatt
      121 ttaattcttt tttcaataaa gcttattgaa aagaacttta acttagaaga gtatcaccaa
      181 attgcagata caatatcata agcagttatt tattgacaac cctttttaat aaatatatca
      241 tttgaagtat actgacgaaa aggagttgaa taataatgga catcttattg gttgaagatg
      301 ataagaatat tcgagaaatg gtatacgatt ttctgattag tgagaattac tctgttaaaa
      361 ctgccattga tggaaataca gctattgaaa tatttaaaaa ttattcattt gatattattt
      421 tactggactt gatgttacca aagtctagtg gttttgaagt attaaaatat atacgaagtg
      481 tgagtgttgt tcctgttatt attactactg cgaaagatag tgacacagat aaaatgctag
      541 ggcttaatct tggtgcagat gattatatta caaagccttt ttcaatcgta gaattgcttg
      601 caagaattaa agctaatatt agacgtgcaa cagtgtacag cagttcaaga aactctcaag
      661 atatggttat tcagtataaa gatttgatta taaacatatc aaaatatgta gtttcaaaga
      721 gtggaattgc cttgaactta acacatacag aatttgaaat tgttaaatta ctagcgacta
      781 atcaaggacg tgctttttca aaggaacaat tatacaacgc tatttggaaa gaaccttatt
      841 atggaaatga aaatgtgcta aatactcata ttaacaggtt aagaaataaa ttgtctgatg
      901 gtatggaaaa aaataattat attaaaactc tttggggaat tggctacaaa atggaggaag
      961 aataatggag ttatttttag ttgtaatttc tattgtacta tttgctctct acttaaaaga
     1021 acgaaataaa tcaaagaaaa tggaaagtga ttataagtat atcaattcaa gacttcgtga
     1081 cataacaaat gatgatgaaa ttaattatat tttagtacca tctgatattt cgattgtaaa
     1141 agaaacggca gtagatataa ataaacttct tgaaaagttt tacagtaggc aaattaatta
     1201 taatagaagt gaaaaagcga tattgcaaat atttacaaac atatcacacg atttacgaac
     1261 tcccatcaca gtattaaagg gttatattga aatgctatat cttcaaagtc aaaaagaaga
     1321 attatcttct tcaatgagag caactgtgga aaaaatgcaa acaaattcaa atgagcttgt
     1381 taattcaata aataacctgt ttaatatggc aaaaatccaa tctggagata tgatattaaa
     1441 tcttcaaaaa tccaatctaa cacaattatg tcatgagatt attttagagt tttatgattt
     1501 gctagataaa gaaaacttta atgttgaagt taatattgaa gatagaccta tatatgcaaa
     1561 tgttgacaca gaagcattac aacgtatact caaaaattta attgataatg ctataaaata
     1621 tggttcagat ggtagatttt tagggatttc actatatgaa aaggaaaaac atgtttttat
     1681 agatattgag gataaaggtt caggtatttc tgaaactgaa aaagagcata ttttcacaag
     1741 agcttatact gctgacagaa aaaaaggcaa tggtttaggt ttggctattt ctcaagggct
     1801 tgcaaactct atgggaggtt caatttatgt aaatagtaga cctaatgtaa aaacagtttt
     1861 tacagtaaaa ttgaaaagtt agaaaaaagt tagatttaaa agagagaaaa gataatttta
     1921 ttttgtatac tgtttttaga aaggagggag tatgatgaac tatatcatag agacccatca
     1981 attaaagaaa atatataaag ataaagctgt tgttaatgct gttaatattc atgtgaaaaa
     2041 aggagaaatt tatggatttg ttggaccaaa cggggcaggt aaaagtacag taatgaaaat
     2101 gttgctaaat cttgttaagc taaattctgg agaaatagtt atgttcggta agaaagtggc
     2161 agaaactgat tttgaaatat taaaaaagat tggtactatt attgaaaatc cttatttcta
     2221 tgagaatttg actgctaaac agaatcttga tttacactgt gaatatatgg gttattataa
     2281 caaagaacat atatcagagg tattggaatg tgtcggattg tcaaagcaat caaacaagaa
     2341 agtttcaaaa tattctttag ggatgaaaca acgtcttgca atagctagag caattcttac
     2401 taaaccagaa ttattgattc ttgatgaacc tataaatgca ctcgacccag aggggataag
     2461 agaaatgcga gaattattcc gtaagttaaa tacagattat ggaataacta tatttatttc
     2521 tagtcatatt ctttcagagg tagaacaaat tgcggacact attggaatta ttcaagatgg
     2581 taaacttatt aaagaaattt ctatgtcaga tatacacaaa tatcaaacag attatgttga
     2641 agtagatgta gataatgtgg aacttgctgg atacttgtta gaaaaagaat ttggaataaa
     2701 agactttaaa attacttcgg aaagttgtat tgaaatatat gatttacgaa aagatgtaaa
     2761 agaaatatcc aaaatattta ttcaaaatca aattggtata aatagcattg gacgtaaaca
     2821 aagttcttta gaagattact tcttccaaac tacaggtaca ggaggtaaag aaacactatg
     2881 atacatttaa tgaaactaga gctaaaaaaa gttgggctaa aaagatattt tttattttca
     2941 atgctgggga ttttcttatg tatgtttttt gtctttactg gattgaatga taaatctact
     3001 tcaatatatg attatgatgt tacatttagg acaatcagtt tatttttctg tttttactat
     3061 attatattat ttgctgtgtt gaatgtggca tatataataa atgaatatac aaataaaaca
     3121 atcttgattt tattctctta tccacttgac cgtaaaaaac ttattttatc aaagttactt
     3181 ttgattacga ctcttatgat atcatcaatg atagttggat atatctgttg ttttacattc
     3241 gttgttgtgt tggataagta ttttaattta gttattggag attttcatat ttcaatttta
     3301 tcttattgga taccaggagc aattaaaagt attattgtat tttgctcatt aggtatatgg
     3361 acattagttg ttggaatgat aagaaaatca atacctgtta cccttgtcag ttcaattatt
     3421 tttatcaatt taagacaatt tattttagct ggtacaaata caactcaaga cagtttgatt
     3481 tctgttttta ttatagttgg tataactgtg attggtatat attatatact aaatcataaa
     3541 gttactgaaa ttgactagat actgtggcta ggaaggtagt tattaattgt atcttcttgg
     3601 gataataatg atttgctatt atatcagcta ttattaatat tattagagtt ttaataatat
     3661 taataatagc tatatatttt ttcttcttat ttataaattt aatgatttat agtcataaaa
     3721 tgaactctaa gaagagataa aaatacgttt tatatgtttt tagattaata taaaatatca
     3781 caatgaaatg ttgtacaaag tgatatataa taattatatg aactataaaa ttgactaaac
     3841 ttattgacta ataaattaga tgaaaagttg agacttactg aaaataaata caatgaaata
     3901 tatactacaa gtattaaatt aaatctgaac tttacatact attagaataa gtattataag
     3961 attataatga tttaaaaata ttaaaataat tgagatgata acatgaacaa ttccttttta
     4021 gataaattga ttgagacaaa agaacttaaa aattcacttt ataatgtgtt aaaacacaat
     4081 tttctttatc attctaataa gatagagggc agtactttta ctactgaagc attagcactt
     4141 ttactcgata agaatgttgt tacaggaaaa catactttag atgatgtaca agaaactgtt
     4201 aattctagtt atgtatttga tacaataatt gactcactga aagagaaaat aacacataat
     4261 ttcttaagga atctacattc aagtttaatc ttcaatacaa ccctaaattc cagggggatg
     4321 tctggtatat ataaaactat acccaatatg atactaggaa ctgatgtaag catagcacaa
     4381 ccatttgaag ttgagccaaa gcttgatgaa ttaattgaat ggtactatag tcagagtgaa
     4441 gtatctatta aagttatagc tgaatttcat tatagatttg aattgataca tccatttcaa
     4501 gatggtaatg ggcgcatagg tagatttgtt atgctaaaac agatgttaga aaataatctt
     4561 cctataaaaa ttgtatcttg ggatagtgag gacttgtata gaaactcctt aaattcttgt
     4621 agtttaggta attatgtccc tttaatcgaa tatttatcaa gtttagaaga ttttagagaa
     4681 gtatataaaa tgttatggaa ataattgttt gcagcaaaaa aaagttttta tataaatata
     4741 tttttatcta tgctatttaa cgctgatttc ttagttattc ttagcttgga ctatatccac
     4801 tattttaact atataaaata gtggatatag tttaactatc tctttataac tattcttatg
     4861 tatcttagtt gtttcttaat atggggacag ttcataaaac taaaagtgat ttttaatttt
     4921 atcatttata atttaccatt acatcagaaa aatacttatc attagaattt tcataaaagg
     4981 t
//
LOCUS       DAKBNN010000242         4971 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000242 DAKBNN010000000
VERSION     DAKBNN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.248"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(189..1520)
                     /locus_tag="OUB02_003493"
                     /pseudo
     CDS             complement(189..1520)
                     /locus_tag="OUB02_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            complement(1565..2272)
                     /locus_tag="OUB02_003494"
     CDS             complement(1565..2272)
                     /locus_tag="OUB02_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887220.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     KVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2443..3357
                     /locus_tag="OUB02_003495"
     CDS             2443..3357
                     /locus_tag="OUB02_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887221.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3357..4118
                     /locus_tag="OUB02_003496"
     CDS             3357..4118
                     /locus_tag="OUB02_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887222.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4130..4867
                     /locus_tag="OUB02_003497"
     CDS             4130..4867
                     /locus_tag="OUB02_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887223.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1697 a          757 c          692 g         1825 t
ORIGIN      
        1 tttcaattaa acaaggtaaa aactataatc ttaaaattaa aattaacatt attcttaaga
       61 agcaagaaat ttagaaagta ggaagtttag tgataagtgg tagtaagtaa gaaatttagg
      121 aagtgggaag tttagttata agtagtagta agtaagaaat ttagtaagtg ggaagtttag
      181 tgataagtat gtgcattaat aatgccagat actatcaaaa tacctagacc atgattttga
      241 cctttggcat atgcctcact tagtccattt tggctcttta ataaactatt aattctttct
      301 tgactcatac cttttccatc atcttcaaca attatatagc aaaaatctaa atcagactta
      361 gaggtaaccc ttatattaca tccttgttgg ttatgagtta tactattttg tattaaatta
      421 ctaatagccc tttttaataa ttttttatct gcatctataa taatatcttc atttagtata
      481 tcaagttcaa cgtaaaagtc attatccaaa ttattattta taaactcaga aataagctct
      541 ctcaaaaacg cagaaactct aatcttatca caattcaatg cttgcatatt atattcaagt
      601 ttagacacta aatttaggtc atttactaat tctctgattt taatcccttg attgcatata
      661 atagacactt gctcttgttc tctatttgaa aggctactgc tttcttcaag ttctccagca
      721 tatccaatta tcattgaaag tggagttctt atatcatgag atattcctgc aatccagtta
      781 gacctagctt cctctttctt cctaagtttt tcatctttca tttgcaactc tttagataca
      841 gtattaatag attttgaaat atcacttagt attccttttt cttgtaaaag tactggctct
      901 ccttttgaca taagctttat gccagtaata attggtttta tagatttcac catttttaaa
      961 cctatgaata gagctattaa aaaagtaact attgcattaa ccaaaaacat acctattatt
     1021 aacataggaa attgttctat ttccttaacc gtataagtaa ggttaaactt agtgtacttg
     1081 tcttttggat aacctgttac taatattcca tttggatgtt cccatacata cacaggatag
     1141 tcctttaaaa agaaccttga aaactttgct acatctgtca aagtataatg acttggaatt
     1201 tcttttggta aatcaaatcc ccatattcta tctccattgt tatcaattaa cattgcccaa
     1261 actttatttt tatgtagtat ttccttacta gaatcatcta aaataaactt tccatctttt
     1321 aaaaccaaat tatcttcaat cataatcaag tttgatttta tttctctgct atcacttgta
     1381 tctaaattat cagagtattt agatacaaat aacatatatg aaaacagaag aaaacctgct
     1441 atatttaata caataaaaag taatgaaatc aagatacttg aacctataaa tcgcctcaat
     1501 atgtacttta cactatccaa aattaaatca ctcctcttga tttcttttat tctaatatct
     1561 aaaattaatt aatcatcaac ttatatccaa gacctcttac tgtaataaga tgtttaggtg
     1621 tagatggagt tttttctatc ttctctctaa cccttctaac atgaaccata agggtatttt
     1681 catatccata catatcatct ccccaaatgg cttgacatag tgaatcatta gtaacaattc
     1741 tccctttatt ttcatacaac tttaaaagca acgaatgctc tttagctgtt aaagaaatct
     1801 cttgcccttc tttctcaaca catgcactat ttaaattcac aacaacagaa tctcccagtt
     1861 taaatactgg caactcctct tcttctatag gcacatatac acgatttaat atacccacaa
     1921 gccttaaagt aagttccttt ggtaaaaatg gctttactat ataatcgtca gcccctaagc
     1981 ccaaaccaat caatctatct tcatcttcac ctcttgcaga gagaaaaatc actggtattt
     2041 tagatgtctc tctaattttt ctcataatag aaaaaccatc tccatctggc aatacaacat
     2101 ctaaaattgc acaatctggc tttacatttc taaatacctt aacagcttcc tcataacttg
     2161 atgctaaaaa tactctacta aatccctctt ttctcaggat ttcatctatc atttttaata
     2221 attccttgtg gtcatcaacc acaagtattt ttttatcttt tattgactcc ataatcatca
     2281 ccctccttta gtataccata tacccatttt cacaactttg cctaaataga cttttaaggt
     2341 tattgtaagg taaatttcag ctaattgaaa tgtaaccaag ttattatact gatataagtt
     2401 aaaagaatca ataagcacag attaaaaaag gggatgaaac atatggaaat gataattaga
     2461 acacaaagac tttcaaagga atacaataaa atatttagag taaaggatat agacctaaga
     2521 gttccacaag gagctgtata tggttttttg ggtcctaatg gagctggaaa atctaccact
     2581 cttaaaatgc ttcttggact tgctaaaccc acacaaggaa atattaatat tttaggtaaa
     2641 gagttaaatg aaaaaaatag aatatcaatt ttaaaagata taggttcttt gatagaatct
     2701 ccatcatact atggtcatct tacagggctt gaaaacatga ccataatgca aagactctta
     2761 gatttaccta aaaaaaatgt aaatgaagca ttaaaaattg taaggttaga aaatcaaaaa
     2821 aataaaaaag ttagtcaata ttctttaggt atgaagcaac gtcttggtat tgctatggca
     2881 ataatgaagt ttcccaaatt attaatactt gatgaaccta caaatggact cgacccagca
     2941 gggattgagg aaattagaga attaataaaa tctctaccag aaaaatacgg gatgactgtt
     3001 ctaatctcta gccatctttt aagtgaaatt gaccaaattg ccacatcaat aggaataata
     3061 aatcatggcg aactggtttt tcaagatagc ataaaagaac ttcataatag aggtcaaagt
     3121 cagattgcta tcaaaactaa gaatgttgta tctgcacaaa aactattaaa tactaaagga
     3181 tatatgtcac ttatacaaga tgggtacttg actattgaaa atttaaaaga ctcagatgtg
     3241 gctaaaataa atggtatgct tgtccattca aatatagatg tatttagaat agaagaacat
     3301 aaaaaaagct tggaagatat attcttagaa cttactggta aggcggtgac tttataatga
     3361 aataccttgg tctagaattt tataaactta aaagaaagaa gattattctg atgacctttt
     3421 tatttgcagc tgttgaaata atgtggtgta tcgtaaattc aaatagagct attacacgta
     3481 atccagatat gttaggtgga tttgaatatt cctatatgct tatgagtttt gcaagtttaa
     3541 atggtctatt ctttcctatc ttaatatcaa taatcacatc acgtataagc gatatagaac
     3601 ataaaggaga tacatggaag cttttaaaat cttctgtgac atctttaaat agtatttatt
     3661 tatctaaatt tttatgttct gctattttgg tatctatacc tgtattgatg caagtgttat
     3721 ctattgtgtt gtttggacat tttagagggg ttattgagtc aatttctgta tctttacttt
     3781 taaaatacac attaggaaca attcttgtaa gtattgctat cattgcacta cacctatgga
     3841 ttgcaactgt aattccaaat caaatgattg caattacatt tggcatgatt ggtagtttta
     3901 taggtttgac atcaggtttg ttttatcaag gtattcgtag attgtttata tggtcttatt
     3961 acctagagtt atcaccttta agttatgctt atgataatgt tttaggttca tctgtctata
     4021 gaattgatat gaatttttct atcacgattt tagttttctt aattggaatg ctaatttatt
     4081 atattggaag aaatcattta ttaaaaaaag aagtatagga gggatttaaa tgttaaggaa
     4141 gtcagtcttg ggagaaattc taaagttaaa aaattctttt atatggtatg tattattagc
     4201 tttgccactt ataagtgtgt tgattggttc tggtaatttt tatctaaatc aaggcatact
     4261 caaaaaagaa tggtacagct tatggactca agttagtctt ttttatgggg aatttttctt
     4321 tcctatctta attgcaatct tttgtgcata tatttgtagg cttgaacata tgaatcataa
     4381 ttggaataat gtaatgacac tgccagttaa tttaaaaaat atctttttat ctaaacttac
     4441 agtagtttca atacttacag ctattacaca agttttcttt gttatctttt atatagttgc
     4501 aggaaatatg tttggttttt ctggtagtgt acctaaagaa ttgactggtt ggattgctaa
     4561 aggttggatt atttctattt caatagcttc tatacaatta tacatgtcta taaagattag
     4621 aagttttgca actcctattg gtattagtct ttgcctttct ctatttggta tgggattaat
     4681 agtagcaaaa cctgctgttg gtatattttt cccttattct atgttaggta gtgctatggg
     4741 ggttataaag caatctgctt taaatgttaa tgacactata gttacaatat gtgttgcatt
     4801 tattactact atagtatttt ctgaaattgc caatagaaat tttagaaaga atgatgttat
     4861 ttcttaatgg taaatgctag atatttttgg gtttgaaatt tatattcttt ttagttgaaa
     4921 tctatatcca ttttatttaa aaatatatat cttttatttg aattttatat g
//
LOCUS       DAKBNN010000243         4957 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000243 DAKBNN010000000
VERSION     DAKBNN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.249"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(6..542)
                     /locus_tag="OUB02_003498"
     CDS             complement(6..542)
                     /locus_tag="OUB02_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCT8887224.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(655..1329)
                     /locus_tag="OUB02_003499"
     CDS             complement(655..1329)
                     /locus_tag="OUB02_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCT8887225.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            1532..3406
                     /gene="asnB"
                     /locus_tag="OUB02_003500"
     CDS             1532..3406
                     /gene="asnB"
                     /locus_tag="OUB02_003500"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCT8887226.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3498..4934)
                     /locus_tag="OUB02_003501"
     CDS             complement(3498..4934)
                     /locus_tag="OUB02_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCT8887227.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKGSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIVFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         1917 a          596 c          714 g         1730 t
ORIGIN      
        1 tacaactata ataaaatata aaattgatat ccaatcaaaa aaataactat gaaatttaat
       61 agcttagata tgaatttttc tgtttttgag ttagttttta taggtagtaa agaaatgttt
      121 atggtaatgg gataaaacaa ttcaatacct tctttagtaa taaggtcaag acacaagtga
      181 gatagatatc ctattataaa cccactggac aaacatgaga aaactatatt attgttagtg
      241 aagattgtca gagatttaaa aaaactataa ataagtaata aggcaattag actgtgagta
      301 aaccctctat gtctaaacct acttccaaat aatttgtata ttaacataaa ttttttactt
      361 atgtaagaac ctctcatatc tatatctgga aataaagaac ctaaatatgc aaaataaata
      421 taaattataa ttaatactat tttatatgca atatcgtatt taactaggta tagattgttt
      481 ataaagggga gtatcaaaag tgcgaatata taacctcccc tttcatgtgt ttcttttatc
      541 atataaaaat cctcctgaat cgtcactata aacattatat tggtaaattt agactatctt
      601 ttattatatt atatagcaaa cataagtttt tttcaaaagg attttaatat ttaattaatc
      661 ttgagatttt aaatttttgt aattttctaa agcaccattc caatttacta cattaaacca
      721 gttgtcaatg tattcatttc ttctattttg atattttaaa tagtaagcat gctcccaaac
      781 atcaagtcct attataggag ttaggttttt acttacaggg ctatcctgat ttggagtagt
      841 cataatagat aatttcccat ctttagtagc tacaagccaa gcccaaccag aaccaaagac
      901 atctaaagca gatttttgga actcttgctt aaatttttca aaagaaccaa agtctctatc
      961 aatagcttct tttaaagatt cagaaggtat ggttttttct ggtgtcatta tatcaaaaaa
     1021 gaatttatga ttataagctc cacctgcatt atttcttaca gttgtagcaa tatctttagg
     1081 taaagaatct aaattttgca ataattcaca taaagaataa ttataaagct caggatattt
     1141 ttcaagagca gcatttaatt tatcaacata agcttgataa tgcttatcat gatgcagttt
     1201 cattgtttct ttatctatat aaggttcaag tgcatcatat gcataaggta atggtttaac
     1261 cttaaatttg ttattttcag gtgtaaaagc aaatgaagtt atgcactgtg agattataaa
     1321 taaagacata ataactggta ttaatatttt tttcttcata aaaatcacct cctaatatag
     1381 tatttctaga aataaaaaaa atatgaaata ttaacagata atttataaga atttaatagc
     1441 aatttcattt taaattgaat aagttttaaa gccaaagaca aaactatgaa atatagaata
     1501 agactaaagg acagaaagga attttgatta tatggacggc aatatttcaa tttattataa
     1561 aggtgatata tctaaaaaag aagatattaa atttaaaata aaagaatctg taaataatac
     1621 aagactttct agtagaatag agtataagga tgaggaaaaa acttttgatt ttgaaagatt
     1681 taatgtaatt gatgctattt atggaaataa accatttgaa aaagatggaa aagttattgt
     1741 ttttaatgga aatatatata actatcatga aataaaagaa gaattaatta aaaaaggaca
     1801 caattttact acaaatgatg atattgaagt cttattggca tcttatatag aatttggtaa
     1861 gaattgtgta cataagatta aaggaatgtt caactttata atctatgata gagaaaatga
     1921 aagtatattt ggagcaagag atttatttgg gataaaacca ctctattata taaataagga
     1981 aaatgcaata attttttcct cagagtataa gtttttactt gaatatatga aaaatttaaa
     2041 tataaatgaa agaagcttac aaagttattt ttcatttcaa tatgtacttc ctgaagatac
     2101 tatgatacaa ggaataaggc tgataccagc aggtcactat tttagggtag aaaatggtat
     2161 actatcttta aagagatata ataaacttga gtttagaagt agtacaaaat ttttttatac
     2221 taaaaatcac ttaggcaata gagatgtgaa cgaggatgat ataagaaata tagtagtaga
     2281 ttcaatagta actcatatgg aagaggagaa agaaattgga acatttttgt ctggtggaat
     2341 agattcatct attataacta ctgtggcttc acaaattaat ccaaatataa aatctttttc
     2401 tgcaggcttt agtgtcaaag gatatagtga attagaagta gcaaagaaaa cagctgataa
     2461 gttaggtata gaaaatatcc agattaatat aacacaagat gaatatataa aatcattacc
     2521 gaatgtaatt tattctttgg atgaccctat agcagaccct tcagaagtag gtctttattt
     2581 cttaattaaa gaggcaggta aacatgttaa agtagcattg tctggagaag gagcagatga
     2641 attatttgga ggatataata tatataaaga gtatagtact atgaaatcag ttgtcaattc
     2701 acctacttat attaaaagta tattaggaag agtttcagaa cttatgccaa acataaaagg
     2761 aagaaattat ctttatagag ctacaacacc attagaaaaa agatatatag gaaatgcaaa
     2821 agtttttgaa aacagcgaag taaaaagatt cttttttaag tataaggaaa agaatatata
     2881 tgaatacctg ttgtctaatt tgtatagaga tgcacaaaaa aataattacg attatataag
     2941 taaaatgcaa catatagatg tgaatacatg gcttcaaggc gatatacttc aaaaaattag
     3001 caaactttct acagctgagc aggttgaact tagagtacca tttttatata aagatgtatt
     3061 tgatgtagca aaaaatttaa gaatggagca aaaaataaat aagaataata caaaagtttt
     3121 acttagagag gcgtttagag aaatagtccc agaacatgtt gttcaaagga aaaaattagg
     3181 atttccaact ccaataagag tttggttaaa agattctcta ggggatattg taaaggaaac
     3241 tatttataat tcaaatgtag atgaatttat agataaaaaa tatgcaataa aacttttaga
     3301 tacgcatctt aaaggtcata gagataattc gagaaaaata tggactatat ttacattttg
     3361 tctatggcat caattattta ttgagcataa aaatgtcgag tattaatact tttttcaata
     3421 ttaatcagag ggcaaaagca tgcaatgttt tcaccctcct gattattatg gaataggata
     3481 agtagaactt tgattgatta gtttactatc tcaataaaat tattggcaaa tattttgaca
     3541 agattacttt caacagctaa atttaagttt tgtccatcat catatccagc ggaaatcaat
     3601 cctagtaagt ttccaaacat atctaaaaga gctcctcctg aactaccact agagattgga
     3661 gctgtaaatt gtatcatttg aaccgtttca aaatcacgaa aagctgaaat aattccatct
     3721 gaaactgtat taaaaagacc taaagggctt ccaattgcaa ctattttttg acctctcaca
     3781 ggttttttac tgactttaac aggtattggc ttgcattttc tattaacctt tatcacagct
     3841 aaatcataat ctgaatggta ttttataatt tggtaagatg tataaacttt atcatcattt
     3901 tcaaatctaa ctaaaaatga atacccatca ttgactacat gaagattcgt aagtatataa
     3961 ccttcattat ttattactac tccagaaccg ctcttaaacg gtttattttc gttattgcac
     4021 acaacaatca taacaacaga agctgataac tttgcaaggt cttcataaga aagttctact
     4081 tcctcatctt gtacaatttt tttagatgtt agatttgtgt tattaagtgt atttttagag
     4141 ttaggtttat tcgtagtagg agaagtctta ttggtatttt cactgtaatt tatatttgta
     4201 atatgccttt caaaaggtat ttctaatata tttggagtct tcctagaaag ttcaaataga
     4261 gtagaaaatt ttgaacattc aggatataaa tcaagaatat ctttatttgg atttatatat
     4321 aaaatatcac acccaagttg tgataaaaaa tataagaaaa aaagttcttg ccttttaata
     4381 tttccaaagt atactatctt tggtgaaata gtagtcttca ccttgttata aaataattta
     4441 ggtagaaaat agtccatcca acacaaaagt ttaactgcaa agtttttgat gatagaggat
     4501 gtacaatttg gcatacttgt ggaaaataaa tctaaagtat ttttaaatac aatcttttta
     4561 gtccaattta aggtgtcatt ttctatttta catggaaagt cgatattata taaatcccaa
     4621 ctgttaaact tagaactatc ataaatatta aaaattttaa gcatcttatc tgtttgttct
     4681 ttgtcaaaat ttatttttag attgtttaat ctaatatagg gaattgtagg attattaaaa
     4741 aaataagtat ctatattttt aattttttct atgtatttat caattgaatc atcaacacct
     4801 atatatcttg taaagtagac ttctttaata tatgaagaac ccttattttt ataggtagta
     4861 tcagatataa attcaattag aggttcagta gactttttta ttgaaatctt taatggttta
     4921 atagtacaaa tcatattttt tctcctataa taagatg
//
LOCUS       DAKBNN010000244         4944 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000244 DAKBNN010000000
VERSION     DAKBNN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.250"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..157)
                     /locus_tag="OUB02_003502"
     CDS             complement(<1..157)
                     /locus_tag="OUB02_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT8887228.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIV"
     gene            complement(382..558)
                     /locus_tag="OUB02_003503"
     CDS             complement(382..558)
                     /locus_tag="OUB02_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887229.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(612..701)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(881..1081)
                     /locus_tag="OUB02_003504"
     CDS             complement(881..1081)
                     /locus_tag="OUB02_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887230.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1083..1433)
                     /locus_tag="OUB02_003505"
     CDS             complement(1083..1433)
                     /locus_tag="OUB02_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HCT8887231.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1595..1784
                     /locus_tag="OUB02_003506"
                     /pseudo
     CDS             1595..1784
                     /locus_tag="OUB02_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2261..2431)
                     /locus_tag="OUB02_003507"
     CDS             complement(2261..2431)
                     /locus_tag="OUB02_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887232.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(2820..3308)
                     /locus_tag="OUB02_003508"
     CDS             complement(2820..3308)
                     /locus_tag="OUB02_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCT8887233.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSITIPNDKNMIITFLSNKS
                     DN"
     gene            4112..4486
                     /locus_tag="OUB02_003509"
     CDS             4112..4486
                     /locus_tag="OUB02_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8887234.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4591..4944
                     /locus_tag="OUB02_003510"
                     /pseudo
     CDS             4591..4944
                     /locus_tag="OUB02_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2001 a          611 c          567 g         1765 t
ORIGIN      
        1 taactatatc aaagcttact ttttgacctt cttctaatga tttaaatcct gaagtttgta
       61 tagctgagaa atgagcaaat acgtcatctc cactttccat tgatataaat ccaaatcctt
      121 tttcattatt aaaccatttt actattccat tattcattaa aaatactcct attaacctat
      181 atcaagatat acttgattta cttgtgtatt accacaaata ctattgtatc atagattaac
      241 ataaatgtct atttaattat aatttttagt taacattttt atttattctt aatatgaaaa
      301 taaatttaaa cattttatat taccaatata ttaatgtaaa tgtagataaa actaaaaagg
      361 gttttctaga aaaaaatctg tctaattata cttacttacc aataccatca cttgatgcaa
      421 gcaagattcc acaacttttt ctccgtactc taatataaaa tcatctaact gtgtatctat
      481 aaaatcacaa taatcatatg aactaccaat ataagcagtt aacatatgtg tacatttatc
      541 cattttatct ctaaacataa tgtatttatc cccctaaaaa actttcatga aaaacaaaaa
      601 gaaaatatct ttttggcaac tggctgacat aacttatttt ataagcgtta gtccctatag
      661 ctttgcgtca ctaaatttct ctagttttgc cgatttagtt ttattctaca gattaataat
      721 acaatagaat tagttattat tcaacatatc tagctgaaaa attaataaat ttatatatga
      781 acgttaaatt atctgaaaaa ataaaaagcc cctctgtcag gagtgactta aaaattgaat
      841 ttaagtttgt atttaaaaca catttttaat aatgcaaaaa ctaatcaaaa ataaacaatt
      901 catcaatagg tgtatctact atttttgata tatctattgc taatttcaaa gaaggattat
      961 actttcctgc ttctaaacgc ataatggttt ctcttctaac tcctactttt tctgctaatt
     1021 gttcttgagt taattctttt aattgtctat atttttttag gttacaagtg aatttaggca
     1081 tattattttt cctctaaaat ataaagtttt atagcataaa catttaaagt gattgcatat
     1141 gtaattgata gtaaaacaca cattaattct atattcctag acactatagt aatacaccat
     1201 aaaatgaata attctataat agcaagctgt cctgtaaatg ccatagctgc tttttcatct
     1261 tgtacatacc tttcatctgc tttatcacca tttatttttg ctataataaa gtttgagaaa
     1321 aaagcaaaaa atccaatata tgtaaggtca gcaacattgc cagatgaaaa atatctaaga
     1381 cttaaaaatc caaggaaacc acaaaaaccc ataaaaaatt ttttctgaat cattttttat
     1441 cctccttagt aattttaaaa gagatatatt tatatcttta tgatacaaat atatctcttt
     1501 tatattcttt tgtcaataga aaatcagtat tatacaatat ttgtactgca ccataattgt
     1561 tagagcaaaa atctactgct tgagatgttt atgtatgact aaatatgatt ttaatttcaa
     1621 attaaaagta gctaaatttt atttgaatag agaaggaaga gatattttaa tagtaaaaca
     1681 atatggagtt tataaccatt ctcaagttga aagatgggtt aatatattag gagagtgtgc
     1741 actaaaaata aaaaaaattt tctatactat atggttcaag ttagatgtta taaactatat
     1801 gatgataact aaatatagtg tacaataaat cgctaagtat tttggaataa acaatagcac
     1861 agttatagca acatgaaaat aaaaattttt acatagtggt gttgatgcct tttctaaaat
     1921 gaaaggaaga ccataatgaa taaaaacaaa aacactaaag aaaaaataaa ttaacaagag
     1981 aacaagaatt agaatatgaa aataaatttt taaaagatga gcttgagttt ttaaaaaaga
     2041 tgtgtgtctc agggaagcca cattaaaaaa cagtggtatc attattaatc aaaagaaagt
     2101 tagatgtatt atcaaaaaat tagaacttaa atgcatagca ttttttcata aatcacataa
     2161 atataactcc tataaaggta taattggaaa agtaaaaaat aataaaacaa atagaagatt
     2221 taaaacatca atagcatatc agaaaataac aaatgatatg ctagaattta taaaatatat
     2281 ctaatgataa aaatggaaaa tcaattataa aaaaggcata tctcgattca ttttttgtta
     2341 tatagtcaaa taagaatact atagatgaaa gatacgattt tgcatatgta ttaagtaagt
     2401 attctgatgt ttttggttga tacacatgca ataaagaagt tgtgtttgca gataaattag
     2461 caaaactata gtataagttg caacaataaa gaaaatgaaa attatcatac taaagcataa
     2521 ggcatgttac aattttatta gataggacat taacatacac taaagtactt tttattataa
     2581 agagtactta tttttttgaa attcattaat gtataaacta tcaagaatat tactcaacat
     2641 aatttaaaaa caacttgaat aggtattttt aaattatgtg atataataaa aaatagaagt
     2701 gtatgccaat acacttctgt agttataact aagcattcta ttcttgagga gtgctttttt
     2761 catttttata accaactctt tttactttaa caataattat cagacctata tatttatttt
     2821 taattgtctg atttattaga taaaaaagtt attatcatat ttttatcatt tggtattgtt
     2881 attgaactac cataatcttc ttctaattta tctagaccac ttttacttat ttctccatct
     2941 ataattatgt ttaattctaa gtattcttca ctaccattag atgacactaa agtgtacgaa
     3001 ccattataag gcatgcctaa attttctatt ttatgtgcca aagggtcttc atcaccaata
     3061 ccagagttta ctgttatttt tccatctttt ttagtccacc atattatgtt tccttcagta
     3121 tattttgatt catctgcata gtagctaaga gacgcacttt ttattgcact tatatcagct
     3181 tcaagtttag ctatctttgc tttttctata tttttaaata atgctggaag tgctactatt
     3241 gccaatattc ctattatagc aattactact aataattcca ctagagtaaa tcctttttta
     3301 tttttcattt atttcccctc catataaaat taataatata taaattttta ttttgttgat
     3361 ttctactttc tatattttac catatataga caaaaaaatc actttctatg acaaatatac
     3421 tattatttta caatttatgg aaatgattta aaatgacatt tcttgttaga aaagatataa
     3481 gaaatggatt aaaataaaga aaactacagt catatcaatg tcaataatat tatttaaaag
     3541 cagatgaaaa taaaaagagg actcagaaaa taatgtctat ttaattatac ttacttacca
     3601 ataccataac ttgatataaa caagattata ctacattttt ttatgctcta gtataaaatc
     3661 atctaactgt gtatctataa aatcaaaata attatatgaa ctatcagtat aataagtcaa
     3721 aatatgtata catgaataca tattttaaaa gatatatatt attacatacc taaaaaactt
     3781 tcataaaaac aaaaataaaa tatgctttta gtaacttgtt aacataactt ataatttatt
     3841 ttataagcat tagcctatat agctttgtgc tactaaatct ctttgattct aacgatttag
     3901 tgctattcta caactaaaat aatacaatag agctatttat tgtttggtag cattgtttgt
     3961 aaattaaaca aatttataca tatgaatatt attttacatt caaaaaaaca gactaaatat
     4021 gtagaatatt tggtaaaact tacctctagt ttttgtctta aaaagctcct attattaact
     4081 taagttagaa gaattaagga agtgacataa attgaaagaa gaaatattaa tagaaaaatt
     4141 attaagagca gaattaatag taatggagta tttatggaaa aaagacactc taatgcctaa
     4201 aaaagaaatt gtaaaagaaa taaaacagat ttatggatgg cataaaagta caataaaaat
     4261 actactaaaa aggctagttg ctaaaaggta tttggctaga tatattataa attctcaatc
     4321 tcattataaa ataatagata gtaaagaata ttatactttt aagaaaaagg tactaaaatc
     4381 tagtaaaagc ataaaaataa tgcgttcact taccactaca cataaaaatg taagtaaaga
     4441 gaagttagac ctattagatg agtattatag aaacttaaaa gaatgatata gtaaaaatcg
     4501 aatgtaatta tttaccttaa ttcttttgtt tgtaaaactt tattgataca gaatttaaca
     4561 tatacataaa ttggtaaagg aatgtatact atgaaaatta gtaaattacc agaagcggaa
     4621 ttaaaagtta tgagatatat ttgggaatca gaaaaggtat taatatcaag agaaatcacc
     4681 aagtctatgg aacaaaaata tgaatggaaa gatacaacta tatttacagt attaaaaaga
     4741 ttactaaaga agagagtttt tagaagcgga gaaaatagat aagtgtacac actataatgt
     4801 attagtaaaa aaaataatat cagagatttt aaacaaaaga atttttaaag aatacacata
     4861 aaaattcaat attaagttta atgtcagcac tatgtagtaa ggatgatgaa gtagatggtg
     4921 atattttaaa tttattagaa gaat
//
LOCUS       DAKBNN010000245         4941 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000245 DAKBNN010000000
VERSION     DAKBNN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.251"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            164..1792
                     /gene="aspD"
                     /locus_tag="OUB02_003511"
     CDS             164..1792
                     /gene="aspD"
                     /locus_tag="OUB02_003511"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HCT8887235.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEGLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1913..2908
                     /locus_tag="OUB02_003512"
     CDS             1913..2908
                     /locus_tag="OUB02_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCT8887236.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3097..3480)
                     /locus_tag="OUB02_003513"
                     /pseudo
     CDS             complement(3097..3480)
                     /locus_tag="OUB02_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3706..3803
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3945..4440
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3945..3973
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4726..4884)
                     /locus_tag="OUB02_003514"
     CDS             complement(4726..4884)
                     /locus_tag="OUB02_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887237.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
BASE COUNT         1755 a          677 c          742 g         1767 t
ORIGIN      
        1 ttttttattt taacatatat ttaataaggc taaatagagt tattatttta atttcatact
       61 ttactttttc tataaaccta ttataattaa agtataatca tttttgcaac ttgtttattt
      121 tattataaaa atgtgaaact tattacataa ggaggaattt aacatgaaaa atcatactta
      181 taaagaaatc aaaaacatat atggaaaaat aagccctttt gaatttaagg acaaattaat
      241 agatattgca aaatacactg caaaagaaaa taacagagaa ttacttgatg caggtcgtgg
      301 aaatccaaac tggacttgtt caactgctag agaagccttt tttacttttg gtcatttcgc
      361 tattactgaa actcgttcta attgggattt aggtcatttg gctggaatgc ctcaaaaaaa
      421 gggtattaaa gaaagatttt ttaaatttat aaatgaaaat atagatatgc ctggagcata
      481 tttagctaga gatattataa actttggtat aaatgaactt ggatttgatg gagatgaatt
      541 tgttcatgaa ctagctgatg gaattattgg tgataactat ccacttccag atagaatgtt
      601 acctcatatg gaaaaaatag tacacgatta cctagttcaa gaaatgaaat atgatatatc
      661 aggtaaatat ggtgatgtag aaatttttgc tgttgaaggt gctacagctg ctatgtgcta
      721 tatatttgat tcattaatgg caaatgaact tctaaaaaaa ggagatacaa tagccttaat
      781 gactccaata tttacacctt atcttgaaat acctaacctt cctcgttacg actttaaagt
      841 ggtaaatata aatgctaatg aagttgatga aaaaggtgct cacacatggc aatatacaaa
      901 aaaagaatta gagaaacttc gtgacaaatc tattaaagct ttattcgttg ttaatcctaa
      961 taatcctgct tctatagcta tggatgaaac atcttgtaat aatctaatag atgtaattga
     1021 aaattataat aaagatttaa tgataatatc agatgatgta tatggaactt ttgtagaaga
     1081 tttttcatcc ttaatgtcaa aactacctta caacactgtt ggagtatatt cttattcaaa
     1141 atattttgga gttacaggat ggagacttgg tacatttgcc cttcataaga aaaatgtttt
     1201 tgataagaaa ataaatgatt taactggaga attaaaaaaa tcagtagata agcgttacag
     1261 tgatatgagt cttaaccctt ctagcctttc atttatggaa agagttgttg ctgatagcag
     1321 acttgttgct ctcaaccata ctgctggtct ttctactcca cagcaagtac aaatggcgtt
     1381 tttctctgct tttgcattga ttgacaaagt agatgcctac aaaaaactca atatgagtat
     1441 ctgccataga cgtcaaaaac tattatttga agctttagag cttccaatta atgaaaacaa
     1501 aaacaatgct gcatattaca ctcaatttga tattgaagaa tgggctaaat taaactacgg
     1561 agaaggctta tttaaattta taagtaaaaa tgcatctcca gtagatgttc tttataaatt
     1621 agctaatgac tattcagtag ttttacttag tggaaatgga ttctatggtc ctgaatggtc
     1681 aattagaata tctctagcaa atctatatga tgaagcttat actaagatag gaaaagccat
     1741 aagagagata ttaaatggtt atatcgttga atggcaaaaa attaagaaat aattatatta
     1801 cactgaaata tctaaattat atgaataaaa gagtttatgt agaccttaac tacttttttt
     1861 gatataatcc tatcggaatt ataaatttta tttatgaaag gcagagaata gtatgagtat
     1921 taagaaaaaa gttgccatag tttttactgg tggaactata tctatgacag tagatgataa
     1981 agtcggagca gctataccaa ctttatcagg tgaacaaata ctatcaatgg ttaccaacat
     2041 tgataaagtt gcagatgttg aagtttttaa ttttgatgaa attccaggac ctcacataac
     2101 accttataga atgatggaat tgaaaaacta tgtaaatgat ttattagcaa gaaaagatat
     2161 tacaggtgtt gtaataacac atggtacaga tagtttagag gaaacagctt attttctaga
     2221 ccttacaatt gacaacatca aacctgtaat agtcacaggt gctatgagaa gtagttctga
     2281 acttggatat gatggttcaa gtaatttatc tgcatcagtt tgtactgcag tatccaaaga
     2341 tgctatggga aaaggagtcc ttgtagtttt aaacaatgaa gtattattag cttctgaagc
     2401 tactaaaaca aatactcttt ctctaaatac atttaaatct ctaactagtg gcccattggg
     2461 aataattgac tgcaacgaac ttgtccttac aagagatatt gtaaatagaa caattataga
     2521 tactgataag gtagaatcta aagttgcctt atttaaagca tatgtaggag aaaatgctga
     2581 ctttataaga tttgctgttg atagtggtta taaaggtata gttattgagg caatgggcag
     2641 aggtaatatt cctcctcaaa tgctttctgg agttgaatat gctagagaaa aaggacttcc
     2701 tgtagttata gtttctagat gtcattcagg tagagtattc gatagttatg gttactttgg
     2761 ttctggtaga gatttaaaaa atcttggttg tatatttggt ggggacttac caggtcaaaa
     2821 agctagaata aaacttatct tagcacttgg aaaaactgat aaccttgatg aaatcaaaga
     2881 tttctttgaa aaaggaatct attgttaatt ctaataactt ctatttaatt atttattgat
     2941 atttcaacat ttttaaatca aaatctaata cttatgtcag ttaaatgtca gcaaaatatt
     3001 ttatccacaa tttttacaca aattttccac aacttatccc caatattatc cacaagaaaa
     3061 aaggtagcac ttatattagt agctaccttt tatttattaa tcttcatctt tccagctttt
     3121 tatccaattt cctatttcta ttattttttc ttcagttatt tcttctccat catgtaattt
     3181 tgaaattagt tctgataatg gattgttatg tagacctcca aaaattcttt ttccctctat
     3241 tttgagatat tctttctctt ttattaatac tctatagtgt acatgtttcc caattctctg
     3301 cgatatgaaa aaaccttttt tagttaatct agctaaaagt gtaattgtag ttgtatgctt
     3361 ccaagcatat gttttttcta tttaatctgc aacatctttt gatattacag ttttataacc
     3421 agtattccat atgtatttca taatttttaa ttctgcatct ggtaattttt tattcaacat
     3481 aatatattcc ttctttcaat ttatgtcact tcctcaaact tatctagctt aaattaataa
     3541 taggagcttt ttatcacaaa aactacaggt aaatttttct atatattcta cgtatttagt
     3601 ctgtttttaa gtgtgaaatt acgttcatac ctataaattt attcaatttt caaacaatct
     3661 tgttgaataa taactaattc tattgtatta tttagttata gaataaaact aaatcggcaa
     3721 aactagagaa atctagtgac gcaaagctat agggactaag acttatataa atatcttatg
     3781 agttatgtta gccagttgcc aaagagatat tgttcttttt gtttttatga aagtttttta
     3841 gggggataat aatttatatg tttagagata aaatggataa atgtacacat atattgactg
     3901 cttatattag cagttcgtat gattattgta attttttgga tacaatttac attccatata
     3961 gttaatataa aacttgtgtt tattgtgata gtgccgaaca ggtgttaata cgatttacat
     4021 tccatatagt taatataaag ctctaattta atagctttat ttttagcaaa atacccaaat
     4081 ttacattcca tatagttaat ataaaaccgg ttccctccga agttcataga gattcttatc
     4141 agcatgattt acattccata tagttaatat aaaacaaatg ctgaaagata attcggttgg
     4201 ggatttaatg aaatttacat tccatatagt taatgtaaaa ctaaatagag cagatgaggc
     4261 ttttcaagaa gccgatagat ttacattcca tatagttaat ataaaactct gatgttgtga
     4321 gatttgaaaa aaacggtaaa ttgaatttac attccatata gttaatataa aacgctgata
     4381 aagttattaa aagtaaaatt gctgaaatac tatttacatt ccatatagtt aatataaaac
     4441 cccaaaataa attgagtatt tccaatgcct acacatatac aaatctctca aatttgcagt
     4501 gaaccatgag tagtgcaatt gataacactt atcacaagtc ctcaatgcct tatatttcaa
     4561 ctgttaagcc ttaatttctt caaaaaatcg aacactgcaa aatttctatg actttattat
     4621 atcataaaaa tattattttt gaatatctgt accaatttgt gatataataa aagcaagaag
     4681 aactacaatc tattttgctg tagagtggag ttcataattt agaaattact tagttttatg
     4741 gaatctgatt tttaaatcaa attcccaacc actcttagtt gccgctttga gtggtttttt
     4801 acgctttcta aatagtttgc tagctatata aactattaag ctagcagata gacttgctag
     4861 tatgccttgt aaaaaattat ccatgtatat tcacctccct tcttagcgtc gggaggataa
     4921 ttttttgata catgaactcc a
//
LOCUS       DAKBNN010000246         4876 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000246 DAKBNN010000000
VERSION     DAKBNN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.252"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            839..1591
                     /locus_tag="OUB02_003515"
     CDS             839..1591
                     /locus_tag="OUB02_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887238.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFSASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1581..3692
                     /locus_tag="OUB02_003516"
     CDS             1581..3692
                     /locus_tag="OUB02_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887239.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            4076..4786
                     /locus_tag="OUB02_003517"
     CDS             4076..4786
                     /locus_tag="OUB02_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887240.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         1869 a          652 c          635 g         1720 t
ORIGIN      
        1 tattacagca tttctattac attgtaaagt cttatttatt acagttcttt attatactac
       61 agacttttat tattacaatc tcttatttat tacagttctt tgctatacta cagaccttta
      121 ttattacagc atttctatta cattgcaaag tcttatttat tacagttctt tattatacta
      181 caaaccttta ttattacaac attttattat tttaacttct ttactataat atagtaatat
      241 tttaatatct caaaatcata tcaatcactt ttcagcatag tctaaaatac ttccactttc
      301 taaaattatc tatcatacct atactaataa tactttttaa cctttttatt ataaatttca
      361 attgttttta acaacctatt tatttccaaa actccaatat attttctcaa ttacattgtg
      421 ttaatagccc ctaaatcctt tcaaattatt tacaatacat tccattaaat aatcttactg
      481 ctctataatt ctttttttct ttttaaccct actatacatt atttacatca tttttttcaa
      541 tacaacattc gttattctat aaccttttta cttttactat atacttcttt tctaactaca
      601 ccttcaatgt tccacatcat attactttag cacaacaatt aatacctaaa aataaccctc
      661 taaatcctcc aataatataa tcaatttaac tataataata acctctcaca ctatccaaac
      721 aatatatttt caaacattac aaaaaaacat ccaactatta caatatagct catatccctc
      781 ctcccctttc aaatgctatc attgacttac aagataaatg aaagtgagga tgatatatat
      841 gcttaaagta aaccaattat gtaaatcata taaaacaggc agtaaaacat atcctgtatt
      901 aaaaaatatt tcttttgaaa tcaacaaagg agaatttgtc gcaatcatgg gttcatcagg
      961 aagtggaaaa actactcttt taaattgtat ttcatgtttt atcccaattg ataatgggga
     1021 aataattctt ggaaataata atataagaaa tttaaaagaa aaggaacttt cagaagttag
     1081 aaatcaacaa ctaggatttg tattccaaga cttcatgcta tttgatggtc ttagtgtctt
     1141 tgaaaatata tgtgttccac aaattatcaa aggtagtcaa gtcttacaaa tggaaaaccg
     1201 tgcaattaat ctatgtgagc tatttggtat tgaacatatc agaaaaaaat accctgccga
     1261 aatatctggt ggagaaaaac agcgtacagc agtagcaaga gcactcatga acaacccaga
     1321 tttaatatta gcagatgaac caactggtaa ccttgatagt aagtcttgtc gtacagtcat
     1381 tgaatcattt ttaaaagcaa agcaagaatt agatgctact atactcatgg ttacacacga
     1441 tagcttttca gcaagcttct gtgaccgagt tattgtgctt aaagatggaa taatttataa
     1501 agaagttgta aaatcaaatt caagacataa attcctagat gaattactag aagttttaaa
     1561 agaaatggga ggcgctcaag atgacaatta ataaattata cagtaaactt cgagcctata
     1621 attggaagaa ctatacaact ttattctttt gtataatact ttctgtggta ttagtaactt
     1681 catatgctct aatttatttt agcccaacaa tacaaaacat acttccaact ggtggagata
     1741 gcagaaaaca ggctacaatg atattctcag ttgctatatt aggatgcact gtctttacta
     1801 catacgcatc ttccctcttt ttaaaattta aaagcaagga aattggtatt ttcatggctc
     1861 taggaacaga taagaagaaa ttaaaaaaac ttctattttc agagatattg ttaatttcag
     1921 tagtatgctg ttttttaggt cttatattaa gtattcctgt atcttttggt atatggaaaa
     1981 tatttcaatt agtgattatt gataccagag aaatgacata tcattttgga actcaaggat
     2041 tcatatatgg atttatattt agtgtatttg tgacattatg catatttata ctttctgtaa
     2101 aatttattaa tcgtacaaat ataattgata tattaaatga ccagagaata tctgaaccaa
     2161 tacaggatgt aaaggactgg tatggtatat taggtataat ttttattata ctaggtattt
     2221 ttctaggata tctcgtacct tcactaacta taaacaaatt aaattataga ctaccatctg
     2281 tatggagctt tacttatcta ctctctctta ttggaatata tatgattatg acatacatag
     2341 ttgtacatgc taaaagagga aaacatccag agaaatacta taaaaacatc atatcaacta
     2401 gcatgatgag atttatggga agacaaacag taaaaaacat gtgtgttatt tcatttttga
     2461 ttgcaggagc attatttgct gtattctatg ttcctaatat ggttacaggt atctatgata
     2521 ctcttaaaaa taaccctata gattatactt ttacctacaa tcaaagagat aaacaggtat
     2581 caaaaaaaga tattgaacaa ttagcagata aacacaatat aaaaattcaa aactatatgg
     2641 aaattccttc tatagaatta attgttgatg gtgtagaatc aatatatcat aaagatggaa
     2701 ctatcgaaaa ggtatatatg aaaaaaagct cctatgatat gttctttaat tctaaagatt
     2761 tttccaagct aattggcaaa ccaataacta taaaaaatgg tgaatatctt gctgttgtaa
     2821 ataaaggaaa tgaaaatcaa gattataaat ataccaaatt tatatcaagc ccagtcacag
     2881 aaaaagaaat cccctttaga tatgctggaa aaataatatt taatggttct ttttttcaaa
     2941 atagaagtat gaacgcctat gtccttaatg atgaggatta taaatcattt actaaagatt
     3001 tatccataaa aaattacaat aattctatac tatttaatgt agataaagat acctatgctt
     3061 ttgccaatga cttaaagaaa gaaattataa aaagaacatc taaggaaatg ggcatgttca
     3121 gtggttatga tgaatacgta aaaaaacgtt atgaatcaac tggaagaaaa tattttatgg
     3181 atgaagaata tccaagtggt gaaggtcatt taaaactaga cccaaacaac aatcagttgt
     3241 atcttaattg gaaatactat cctaacttta aaattttaca aagccaagat atgataaaaa
     3301 acatggcagt cttcttaatg ttattcatat atgtggcaat catatgtttt acagcagtag
     3361 gaatcattgc atatacaagg ggtgttacta tagctataaa tcacaagcag gtctttatgg
     3421 atttaaatcg attaggagca aacaatgatt atataaagtt ctgtattaga aatcaattaa
     3481 agaaagtatt ttcataccct cttattgcag gaagtttttt aatttatctg tttaatttta
     3541 tgattatgtt aaataatgat ggtagaatcg tcaatagtga ggtcaatgct cttttgataa
     3601 acttaggttt tatagggatt tgtggggttt atatgtacct tatatatcta ttaacttata
     3661 agaaattcaa aaaaattatt ggtattttat aataaaaaag gttattagat ttttaaagta
     3721 ataaaatcat tggtgtttta taataaaaaa ggttattaga tttttaaagt aataaaatca
     3781 ttggtgtttt ataataagaa aggttattag atttttaaag taataaaatc attacttttt
     3841 ataataataa aagcatttgt attttataat aagaaagatt gttagatttt ttaataagaa
     3901 aatcattagc acttcagaat aagtttaata gaatagtttt aagtgtaagt atactaatat
     3961 aaaaaatttt attttgctat ctggatataa ttgttatttc attttatata tctaggtagc
     4021 aaaatatatt atatacttaa ctatataaat ataaaatttg aaaggagata aaaaattgac
     4081 ttcgatttgt atttgtgatg atgaaaaaca acaaagaaac atgctgaata aaatcattac
     4141 aagagaaatg gatttgtggg gctaccccta ccaaataaaa gaatattcaa atggagaaag
     4201 tctagtatac aatctagaac acgaaaatta ttacatagat attatttttt tagatattga
     4261 acttggaaaa agtaatggcg ttgacatagc aaaaactata cgaaaacatt caaaggatac
     4321 tataatcatc tttgtcacag gtttttctga ctatgtattt catggatatg atgtaggagc
     4381 tttgaactac atcttaaaac cttataaaac tgaaaaaata tgtaaagtat taaaagaagc
     4441 actaaaaaaa ttagaacaat ggaaagaaaa ctttattaca gttcagattg gaactaatct
     4501 ttgtaaaatt aatacaaaag atatcctcta ctttaaaagt gagctaagaa aattaactgt
     4561 agttaccttt gataaaacat tagaatacta cggaaaatta aatgatatgg aaactgttct
     4621 tccctctaat tttatacgta cgcatcaaag gtatattgtc aatctaaacc atgtagatag
     4681 tataactcaa acttatgcca aagttcagaa tgaaaaaatt cctataagcc gtaaaaaata
     4741 tcaagaggtt gtaggaaaat ttacaaggta tatgttagaa tcttaatttt tgaagttcaa
     4801 tctctagaat tttatttttg aaacttaatt ttttaaagct tagtttttag agttttgttt
     4861 tttcaaattt aatttt
//
LOCUS       DAKBNN010000247         4821 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000247 DAKBNN010000000
VERSION     DAKBNN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.253"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            167..355
                     /locus_tag="OUB02_003518"
     CDS             167..355
                     /locus_tag="OUB02_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887241.1"
                     /translation="MNYHTKEELVEALRVISSSIINCEKGQKKFSEETSHHTCFKNII
                     EAIYISKSLIMDEISKRD"
     gene            615..1814
                     /locus_tag="OUB02_003519"
     CDS             615..1814
                     /locus_tag="OUB02_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HCT8887242.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1866..2369
                     /locus_tag="OUB02_003520"
     CDS             1866..2369
                     /locus_tag="OUB02_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887243.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            2476..2793
                     /locus_tag="OUB02_003521"
     CDS             2476..2793
                     /locus_tag="OUB02_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887244.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2951..3910
                     /locus_tag="OUB02_003522"
     CDS             2951..3910
                     /locus_tag="OUB02_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCT8887245.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            4025..>4821
                     /locus_tag="OUB02_003523"
     CDS             4025..>4821
                     /locus_tag="OUB02_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887246.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKRYLYN"
BASE COUNT         2014 a          349 c          814 g         1644 t
ORIGIN      
        1 aagtattata tgtatattaa taattattaa aatatgtaat gtcactgata gttagaatat
       61 ttaagtgata aaagatgtgt ataaataata atgatgtcag tttataatga tattaaattg
      121 aggtatactg tttctctaaa ttaaaaaata aaggagtaaa tgtgttatga attatcatac
      181 aaaagaagaa ttggtggaag cactgagagt aatatcttca agtattatta attgtgaaaa
      241 aggacagaag aaattttctg aggagacatc tcatcataca tgttttaaga atattataga
      301 agcaatatat atttcaaaat cattaataat ggatgaaatt agtaaaagag attgaagctt
      361 attttgcaat ttaacatgtt ttaatataca ttttattata taagaaaata agtaaatata
      421 catgttataa aatattggaa taatatacaa ttataaattt gttaacatta atatctatat
      481 gttaaaatat agtagaagat gttagtaaat aataggatga gttctatttt aatacaatta
      541 aattaagctt agtttgaaat ttgtatctaa aaagtaaagt aatctagaat taagaaaatg
      601 gagatagaat tataatggaa aaattatatt atacaaatca gtatataaga gattttacag
      661 cagaaataat tgaaataaaa gaagtagaaa ataagtatca tgttttatta gataagacag
      721 ctttttttcc tggtggagga ggtcagtttg gagacctggg aaaaatagag aatgatgatg
      781 taataaatgt gtatgaagat ggaaaaaaaa tataccatgt attagataaa aaacctataa
      841 agatacataa ggtaaagtgt agtattgatt gggatagaag agaagatgga atgcatcaac
      901 attttggaca acatgtattg tctggatgtt tttttaaaac ttttaatgca aatacgacag
      961 gttttcattt gggaaatgag tatagtacag tagatataga aggtaattta gaagaggata
     1021 aaattagaga gatagagttg tttgccaatg aaattattcg tcaaaatata caagtagaga
     1081 cattacttcc aacgaaaaaa gaactaaaaa agatttggtt aagacgtgat ttgccaaata
     1141 ctgatgaaga gataagagta ataaaaatag gtgatttaga tacgaatgca tgttgtggag
     1201 tgcatccaaa atcaactggt gaattgagaa tgataaaaat aaagaaatgg gaaaaacata
     1261 agtcaagtac aagaatagag tttctagcag gtaaaagggc agtggatgaa gtattgaaga
     1321 gggatatata tttgactaaa atatgtagat atttaagctg tagtgaggaa gatgctataa
     1381 atggtattgt aaatctaaat agtaaagtgg aagaggcttt aattaaaaaa agaaggcttg
     1441 aagaagtagt atctacatat caggtcaaag aaatgattga aaaagcaaat aaggttggca
     1501 atatttcggt cattaaaaaa atttatgatg atgaagattt gaaatatgta aataagatag
     1561 caaataaaat tgcagaatat gaaaatagta ttgcattatt agctgttaaa tttaatgata
     1621 aaataaattt agtttttgca tgttctaaaa atttaaaaga tattaatatg ggagttttat
     1681 taaaggatgc aataaatctt atagatggca agggtggtgg aagtaaagta ttagctcaag
     1741 gaggaggtaa gaataatgga aatttagagg ctacacttga ttatgcattt atgaagatag
     1801 aaaaaactct ataaatatat tatataaaaa accttagtag ataaaataga tatggaggaa
     1861 taagtatgga gcaacaaaag gactacatag agttatatga aaatgaagaa ctaatagaga
     1921 taagagaagc acgtatggat gatttggata caatagcaaa atttaattat aatttggcta
     1981 aagaaactga aggaaaagaa cttgatatgg atgttttaac taaaggtgta aaagcattgt
     2041 tattagatga gagaaaagga aaatatcatg tatatacagt atttgataaa gttgttgcac
     2101 agattatgta tacatatgag tggagtgatt ggagaaatgg aaatttcctt tggattcaga
     2161 gtgtatatgt agataaggaa tatagaagaa aaggtatctt taattattta tttaattata
     2221 taaaaaatat atgtgataaa gatgaaaata tagttggaat gagattatat gtagaaaaag
     2281 aaaacataaa tgcaaaagca acatatgagt ctttaaatat gtatgagtgt gattataata
     2341 tgtatgaata cgaagtaata cattcgtaat ataaatttgt aatactgtta tactgatgtt
     2401 ataaagtatt gattatattg acgattatat gaaataaact ggatattata attagaattt
     2461 ttagggggta taaaaatggg cgttttaaaa ggaatgtttt atgtattttt atttttagat
     2521 ttagtgtcaa tattctgttt cttttttaat aaaggaaaaa tagcatctaa taaaattgtg
     2581 tttaatgcaa taggagtact tacatttgta ctttgtttta tgctttttag ttattatcct
     2641 aataataatc ttattggaaa atttattgct tctctatttt ttatatttgg agttgcagga
     2701 gttatgttga aagaaaaaaa ttttttatat gctagacttc ttttaactgt agttatagtt
     2761 ttttctacat taagattgtt cgttattcaa taaaatgtat ttgtatgaaa atttattaaa
     2821 gtctacacat ttaagtgtag gcttttttga ttttttgttt gaaaattaag gaaattttaa
     2881 ggaaatttta aaatcgtctt aaatatttat tgatatactt aaaatattaa tagcgaaagg
     2941 agacttatca gtgatagaaa gtaaaaagtc agaaaaattg ttactgtcaa ttatatacat
     3001 aatatttttg gtatatattt taatgattat aagaataatt ttatttagag atgtaccaat
     3061 ttacgctatt ttcaagggaa cgtttaggaa tgtaaattta attccatttt atacaatata
     3121 tcaatttata gtagatagta atcttgactt tatgaaagct actgtaaata ttatagaaaa
     3181 tataggtata tttattccta tgggaatttt tttacctata gtatgtaaaa atctaaataa
     3241 aaaaactata attatagtaa ttatacttgt tagcctagca tttgaattaa cacaatatat
     3301 ttttgctctt ggaagtagtg atatagatga tgttatttta aattcattag gtggcataat
     3361 tggaattaat atttacataa acatgaataa attatttccc aatgatatta acagatttaa
     3421 ggctataata ggtactagtt tagttttagg agttatagga ttaggagtta tttctaaaaa
     3481 ttatcataac ttactaactt ttaaatttag acctgataga aaaatatcaa aaatattaat
     3541 tgaagaaaat agggaaataa ttaaagatat aaataaagat acagtagata tagttgggac
     3601 atttgagagc tttaaaaaag gaataataac tattaaagca ggttctaata acaaagtaaa
     3661 gagaccagag aatttaattg attctgatgg aaatataaga atttatttaa atgaaaatac
     3721 aaaattagta tcttatataa ttaccaaaga atcaaaaatg gatatagtta aatatgagga
     3781 atttgatgtg aagaatttag atttattaag gagatatgat actattaatg tttggattga
     3841 taaagaaaat cagccaaagg ataaacatgg tattatggct agtaaattat taattggatt
     3901 gtatgaataa aatattaaac aagaataaaa gaataacaaa tataaaaaac gaaaaaacta
     3961 tttctataat tatttttacg ttgtgcataa aataagaaca ttagtgaagg agtagagata
     4021 aattatggag ataataaaat atatcttgta cttttttata tattcatttt taggatggac
     4081 agtagaaagt ataggatgtt ctatagcaag caaaagaata atcaacagag gttttttaaa
     4141 tggtccaata tgccctgttt atggttttgg agcggtaatt gtgatatcac tattgggaag
     4201 atttaataat gtagtaatag tatttctatt aggaatgatt ttaactacga tactagaata
     4261 ttttacagga tttattttag aaaccttatt tcatgctaaa tggtgggatt attctgacag
     4321 aaaattcaat ataaagggta gagtatgttt aaaaaatgca atatactttg gtgtaatgtc
     4381 tgttttaata ataagattta tacatccatt tataaaatat tttgtaagta taattccata
     4441 tagaatatta atttcaatgg ctataattac aacactttgg actatacttg atttgattgt
     4501 tactattata acacttaaaa aattagatat aaagttaaac ttattagacg atataattac
     4561 agaccttaat gatataaatg tgaaattgga taaatttgat agaggagaaa tacaagcttt
     4621 atttaaaaca ataaataacg acgaattaga agttagagaa aaaataaaca aaataaatag
     4681 taagttggac cgtatagagt ctaatattat tttacaaaaa agagttataa aagcttttcc
     4741 acatataaaa cataaaaaac aacaagagca actagaacat tttaaaaaaa ttattaatga
     4801 gaaaaggtac ttatacaatt t
//
LOCUS       DAKBNN010000248         4809 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000248 DAKBNN010000000
VERSION     DAKBNN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.254"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     misc_binding    365..603
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            667..2586
                     /gene="thrS"
                     /locus_tag="OUB02_003524"
     CDS             667..2586
                     /gene="thrS"
                     /locus_tag="OUB02_003524"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCT8887247.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3354..4445
                     /locus_tag="OUB02_003525"
     CDS             3354..4445
                     /locus_tag="OUB02_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCT8887248.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1891 a          480 c          858 g         1580 t
ORIGIN      
        1 ttatttgatt gtgttactta ttaatagtat ttaagtattt actgtactac ttattaacta
       61 tattaagtat tgattgtatt taagtataca aaacctaaaa agagtacaaa aattagaagt
      121 tagttttaat aaaaattagt tttagtattt atctagtcat atctatcatg catgtggata
      181 cgttttaaaa attttaaatt tatgaaaata ttataatatc actataaatc aagagattaa
      241 tttaatataa aaatataaga atattggttt acagctgatt gagatgaaaa ataatgggta
      301 attaatatat tacattaatg ttgattgatg tataactatc tgttataatg aaaatatatt
      361 aaaaatcata gtgttgaaga ggaagagtag agtagaaacc attctcagag aatttgggtc
      421 tatggctgag agcccatgta ataggtctat ttgaaaacta ccttggagct gagcttatta
      481 gcagtatttg tatttgtact atacgtgaaa gtgctcgggt gacgacctta tagtcatcaa
      541 gagggttgta tatacttgta tacgccaact tgggtggaac cgcgaattaa tatcgtccca
      601 aacatatttg gggcgatttt ttattacaaa aaatctaaaa attatttcaa taaggaggaa
      661 aaaataatga ttaaagttgc tttaaaagat ggctcaataa aagaatttga aaatgctatt
      721 tctgttatgg atgtagctaa atccattagt gaaggattag ctagaaatgt ggtagcagcg
      781 tctgtgaacg gagaggttgt aggtctggac cacattatag atactgattg tgacttgaat
      841 ttatttaagt ttgaagataa agaaggtaag gaagttttta gacatacatc agcgcatata
      901 ttagctcaag ctataaaaag actttaccca gaagcaaagc ttgctatagg accaagtata
      961 gaaaatggat tctactatga tatagattta gaccatagat tagttccaga ggacctagag
     1021 aaaatagaag ctgaaatgaa aaaaatagct aaagaagatt taaaaataga aagatttgaa
     1081 cttcctagaa atgaagcttt agagcttatg aaagaacaag gagaagacta taaggtagaa
     1141 cttatatctg atttacctga aagtgaaata atatctttct acaagcaagg tgattttact
     1201 gatttatgta gaggaccaca tttaccatct actaaaaagg taaaagctgt taaattacaa
     1261 agtgtagcag gtgcatattg gcgtggtgac gaaaataata agatgcttca aagaatatat
     1321 ggaacttctt ttgagaaaaa caaagactta gaagagtact tacatttact agaagaagct
     1381 aaaaagagag accacagaaa attaggtaaa gaattaggat tatttatgat acctgaagaa
     1441 ggtccaggat tccctatgtt tttaccaaaa ggtatggaac ttaaaaatga attattaaaa
     1501 ttctggagag aaatacacag aaaagctgga tatattgaga tagaatctcc aatcatatta
     1561 aatagaaaat tatgggagac ttcaggacac tggtatcatt acaaagaaaa tatgtataca
     1621 gttaaaatag atgatgagga ttatgctata aaacctatga actgtcctgg tggattaatt
     1681 tactataact ctcaactaca ttcatataga gatttcccaa tgagagttgc agagcttggt
     1741 agagttcata gacatgagtt atctggtgca ttacaaggat taatgagagt tagagcattt
     1801 actcaagacg attcacatat attcatgtta ccagagcaaa ttaaggacga aataaaagga
     1861 gtagctaact taatagatgg tatctacaaa acttttggat ttgaatataa tttagaatta
     1921 tctacaagac cagagaactc tatgggaagc gacgaagagt gggaagcagc agaaaatgga
     1981 ttaagagaag ctttagaaga gttaggtcta ccatatacta ttaatgaggg tgatggagca
     2041 ttctatggtc ctaagataga cttccatcta aaagattgtt taggaagaac ttggcaatgt
     2101 ggtactatcc aattagatat gcaacttcca agacaatttg ataatactta tattggtcaa
     2161 gatggtgaaa agcatagacc agttatgatt catagggttg cttttggtag tatagaaaga
     2221 tttataggta tattaataga gcattatgct ggtaaattcc cagtttggtt atctccaact
     2281 caagttaaga tacttcctat atctgataaa tttatggatt atgcaaatga agttaagaaa
     2341 gagttatttg ataagggtat aagagttgaa ttagatgata gagctgaaaa aattggattt
     2401 aagataagag aagctcagct tgaaaaagtg ccatacatgt taatagtagg tgaaaaagaa
     2461 gtagctgata ataatgtttc tgttagatca agagataaag gtgaaatagg tagtattaaa
     2521 ttagatgaat ttatagctag tatttcaaaa gaaattgaat caagagaaag tattattcaa
     2581 gactaatata tattatttta gtagctaaga tattgttata taagataagt ttaatcaata
     2641 tgttgatatg ttgatgcata gtgattaaat ttatgaaaag atgagtataa ttagaggtac
     2701 agttaatcat atttttatga ttaatgtacc tctagttttt tgtatggtag atttattaat
     2761 aaatatatca tagaagtgtt ttgaacaagg aaatatttat gtgcattctt taaataaaag
     2821 tttatttttt gagtgataat taagtgcttg ttggtaaggt tatggctgta aaaatcataa
     2881 ctttattttc atatttgttc cattttcata aaaattatta tacctttaag aaaatttctt
     2941 aatcctcttg aaatatttcc tttttagtct aaagttaaat ttctttcttc aaaatgaata
     3001 tttaaatgaa ctaaaattta agtacatttt ctaatcttat gagtctattt ttcatagata
     3061 attgccctat aatagaagaa tctatgttgg tgctatgtgg tgacaataac agcattgtca
     3121 gtgattaatt aatgctttat aaatgattaa ttagtgtttt atgttatttt tggacttacc
     3181 ctctatacat agattaattt atttaacttt agatagagag agaatgtcaa gtacatacac
     3241 agacattaga gtttaagaaa gtgtttatgg agcaagaact tttaaaagag gtagtgactt
     3301 agtttaattt caagaaatga taaatacaaa agaggaaaaa gagaggtgtt gaaatgatag
     3361 aaaaagtaga agaaaaatct aagattatta aaacatcaaa agaatataag cgaactataa
     3421 ttgaagaaaa agataaagaa tggaccaaaa aattaaatga tatacttagt agaagtagtt
     3481 tttctaatga tgaattagat aaaatcaagg cgttaattcc aaaagagatt ttgactaatg
     3541 aaaatgttgg agaagttgtg actgaagatg gatatgctaa gcccgagtat gatgaggtat
     3601 ttaaatcatt gttcacttta aataatgaat atgatttact tgtaaatttt ataaatgata
     3661 tcctaaaaga tgctccatat gctaacagga atatcaaacc gttcactcaa ataaagagaa
     3721 taattagagt tgaaactgac ccaacaatta attacattgg agagaaaaga cctagacttg
     3781 atattttggc tgaagatgaa gaaaataatc atataaatat tgagatgcaa agagcttttg
     3841 agaacgatta tttggaaaga gctgaatatt atcttagtcg tgtacatggt agaaagttag
     3901 aagaaggtaa agagtataaa gaaattggaa aaactattgg aatacatata ctaaatcatg
     3961 taaaatataa tcacatagaa gattatgtaa attgcctgag attaacaatg gacgggcatc
     4021 cagatattta ttctagtaaa actgcattat attttataga gcttccaaaa attcataaat
     4081 ctgactatat agtaaataga attatgctat ggggaaaact tattagtaat ccttctagct
     4141 tagatgtaag agttatagct aagaatgatt ctattattaa aaaagcactt gatagattaa
     4201 aagaattagg tagcaatgaa gaatatttat taaatctgaa aagaggtgct tatatcatga
     4261 ataaaagtag aaattttaaa gaagagattg aaagagagac agcaattaga gttgcaaaag
     4321 aaaaagatta taaaataatt ataatgttga aaaaaaatgg ttttaaatta gatgacatat
     4381 taaataaatt taatgatttt gacttaagtg aagatgagat aaaagaagta tatgaaaata
     4441 attgatgata aaaagtgaac ttatataagt ttgctgaatc ggagtctgga aatatttatg
     4501 tgtatttttt aatgaataaa aatttatttt aaaaacaata atttagaggt acagttaatt
     4561 atatttttat gatactgtac ttctagtttt ttctattgta gatttattat ttactaataa
     4621 atctacaata gaagtgcttt tgttgtaaga atcattttat ctaatgattg ttttttataa
     4681 tagttaaatt tccatttgta gtgtttaaat ataaatctat aacagcattt ctataaatgt
     4741 tataaatcac tataatgatt caactcaaag ttactgctta aagaagcaga gattttatca
     4801 aggtatatc
//
LOCUS       DAKBNN010000249         4793 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000249 DAKBNN010000000
VERSION     DAKBNN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.255"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            11..622
                     /locus_tag="OUB02_003526"
     CDS             11..622
                     /locus_tag="OUB02_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCT8887249.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            920..1258
                     /locus_tag="OUB02_003527"
     CDS             920..1258
                     /locus_tag="OUB02_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HCT8887250.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSNKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1665..3101)
                     /gene="miaB"
                     /locus_tag="OUB02_003528"
     CDS             complement(1665..3101)
                     /gene="miaB"
                     /locus_tag="OUB02_003528"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCT8887251.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3349..4785)
                     /locus_tag="OUB02_003529"
     CDS             complement(3349..4785)
                     /locus_tag="OUB02_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HCT8887252.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1612 a          736 c          533 g         1912 t
ORIGIN      
        1 ggaggtaaaa atgataaacg atattaaaaa taaattcaaa aattacaaac catatataaa
       61 tggatacaag aatatgaaga gagcatctgt tttaattcca atagtagaaa taaataacac
      121 acactatata ttatttgaag ttcgctcaaa aaatttgaag catcaaccta gcgaaatttc
      181 ttttccaggt ggtaaaatag aatcaggtga gactccatat gaagctgtta ttagagaaac
      241 ttgtgaggaa cttggaactt ttagtgacaa tatagaagta atatcaagtc ttgacctcct
      301 tataacacca gtaaagttta tcatacatcc atacttagga tatataaaaa atattaataa
      361 tttaaatata aacaaggatg aagttgacca tacttttttg gttcctataa aatatctttt
      421 agagaatcct ccaaatactt atagtaatac tgtaaaagtt tttccagatg aaaatctacc
      481 atatggttca attccaaacc aaaaagatta taaatttgaa agtggaagtt atcctattct
      541 gttttataaa tataaagatt atgtaatttg gggaatgaca gcaaaaatac tagaaaactt
      601 tttaaatttt ttatcagaat aacactcata ataaatcatc aaaactttta cataagaaat
      661 ttataaaagt ttctaaattt aacaattcta aaatatcaat aactatattt tgtagcactt
      721 ttttatttta ttattgtttg aatatttgat ggttttcaat gcaatttcaa cataaatact
      781 atgtattagt gtatactgtt gatgaattaa agtatgataa tctatttaat aaagtatgaa
      841 gtaaatgatt ttaatgtgtc tttatggtaa aattactaat aagtaataaa attaattaaa
      901 atatatggag gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt
      961 tttcatttta agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa
     1021 ctctatgagt aataaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag
     1081 cattgttact tttattgtct ctataatgct atttattatg gcgtttttgg gatatagaat
     1141 agagagtggt caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt
     1201 catactttcc agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat
     1261 agttgtaagt tgagcagtta attataaaag attgactgct ttttcatgta tattgactac
     1321 tcttattaaa tattttaaca tttaaaaatc tatgtaaaaa taatgaatat agtacaaact
     1381 tctaataatg attttacctt tgtggttaca gtccactata tataatttaa tatgataaca
     1441 gggaattact aatgacaact aagtaaactt ttataaaata taataaaaca aaaatagatg
     1501 tacaatgaaa ttatatctta tatcaaattt atttttatta atagcaaaaa cttacattag
     1561 tataacatat gacaaattcg cttaaaataa aaatttttaa tataatttgt tctatagtac
     1621 atctattttg attagtttgc ataattttat tattttgtac aatattaatt atttactaat
     1681 atcccattta atgaaaaagt tctaggttca gtaattttga ccataactaa tttaccaatt
     1741 aaatcatcat ttccaccttc gaaatttacg agtttatttt gtctagttct accagcaaac
     1801 ttattttcat tatttttact tcttccttca actagaactt caactattct atctttatat
     1861 ccatcattta tttctgcaga aatcctattt acagcttcta acaccctatt aaatctatca
     1921 tgctttatat cttctggtat ttgattttcc attttagcag caggagttcc ctgtctttta
     1981 gaataaataa atgtaaaagc tgaatcatat ctaacttttt caaccacatc taaagtgtcc
     2041 aataaatctt cttctgtttc tcctgggaaa ccaatcatta agtctgtaga aaaagcaata
     2101 cctggaactt cctttttagc tttttctatt atttctaaat aatattcctt tgtataatgt
     2161 ctattcattt ttttcaaaag gctactgctt ccacattgta ttggtagatg taaaaactca
     2221 cacaccttat cacaatctct aattgcgtat attacttcat cagatatatc ttttggatga
     2281 gatgtcataa atcgaaccct ttcaattcct tctatatcat tcacttttct taaaagttct
     2341 gagaatgaaa ctggattttc aagtgtctta ccataagaat ctacattttg tccaagtaaa
     2401 gtgatttctt tcgttccatt tgcaacaagc tcttttatct cgttaattat atcttcagga
     2461 gttctacttc tttctcttcc tcttgtgtat gggactatac aatatgtgca gaaattattg
     2521 catccataca ttatatttac aaatgctttt aattcaaatt ttctattact tctaagacct
     2581 tctattactt ctccatcaac atcccataca tccactaaca ttttgtcaga atttattgat
     2641 tctgtaagta attgcggaaa tttatataga ttatgtgttc caaatataag gtctacatgt
     2701 ttatattttt ttcttaattc ctctacaaca tgtggttgtt gcatcataca tccacataca
     2761 gctattttca tatctggatt ttttcctttt agatgtttta attgtcctaa gttaccatat
     2821 acttttagct ctgcattttc tctaactgca caggtgttat atattattaa atcactttct
     2881 tcaaccatca ttgacatttg gtatcccatc tcttctaaca ttgaacataa tttttctgaa
     2941 tcatgttcat tcatttgaca cccgaaggtt tgagtaaaca cgagttttct ctttccagtt
     3001 ctgttgaagt attcttcatt ttgtctgtgt atttgttcta tatatttatc ttgttcttgt
     3061 attttctcta taggtattgt tacttgtttt cttttgctca cgtctattcc tcctagttaa
     3121 tttcttatat tacacataac cttaattata tcaaataaga aaaatctttt aaagatatta
     3181 ttatttttct agttgtgttt taatagtaaa tctaatgatt tatatatact atatttctaa
     3241 aatataaaat catataatta ttataataat aaaaagatgt tttaaattaa aacttaaaac
     3301 acctttcctt aaataaaata tatgatttac tattaaatac aattacatct attcattttc
     3361 aattaaataa caatcattta atattaaata ttgaccttcc tcaaccgtta tatatcttct
     3421 tcctgaaaaa gtatcattag taactattcc ctctgcatta cctaagctat catttctaac
     3481 ttcataatat gcatcagaag aatttgacat tataatatat tctccagcag gaatttctaa
     3541 tccaatttta tacataccat ttccaaactt accattcttg gccttctggg ctttagcata
     3601 ctttgctaat gtcattgttg aatcctcaat tttaatatat tgaccattct ttaaggtgat
     3661 atatctagtc ccactaaaaa tatcatttga aagtatacta tcagcattac ctgtactatc
     3721 acttgtaact tcataataag aagcataact tcctgagttt gaagttatta agtattcacc
     3781 tgctgagata tcttttccaa ccttatacat tccatctttt atagtctttg tagtaggttt
     3841 aacatttttt acaaccccag tattaatatc tccaattcca tttgttatat ttaaacactg
     3901 ttgtactata tttgaatcta tatatcctat agaagttatc ttcttagcat tttttaaaat
     3961 gcttttattg ctaccatcat taactaaaac cattggacca tgttttgata aagaagatcc
     4021 tacaagtgca tttgttaatt cataagctcc agctatataa aactctctag catccttata
     4081 aaacttattt attattgctt tattagtttc aaaacgagta ctccccccta gtctagtaga
     4141 tttagtattg tttactaaag tggtactcat tgaagctgtt cctcctatta cataagactt
     4201 taaaccacta gttgaaaaag gtatactttg tccattagta agaattattg gtgctttatc
     4261 tcttgctgct acagatgcaa tacttatagc atctgcttct cctttatatg cattggttag
     4321 cataacagta ttgacttttt ttattgaatt tatttctttt gcaacattat aacttgtttt
     4381 tattctgtca tttcccttaa ttcttacaac tttcattttc ttagatttta atgaattttc
     4441 gacagatgta ctaatagaat atgttcctcc aattatataa acttttgata cattttttaa
     4501 tcttgcagat gtctcagttg gtatagtatt tttttcagtt aatagtatag gggcatttaa
     4561 agctcctgct agtccacttg cacttaatcc atctgcaata gagttactag tattaattaa
     4621 tatagcagtt ttatatgcat ttttatctgc tattttacct gcgatttcat acttattttt
     4681 tccttgtatt ttttctacag atgatattgc atctactaat tgaatatttg caaatacaaa
     4741 agaaatactt aaagtcaatg ttaatattct tttatagaat ttcataataa aat
//
LOCUS       DAKBNN010000250         4729 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000250 DAKBNN010000000
VERSION     DAKBNN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.256"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..762)
                     /locus_tag="OUB02_003530"
     CDS             complement(<1..762)
                     /locus_tag="OUB02_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887253.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKA"
     gene            complement(763..1638)
                     /locus_tag="OUB02_003531"
     CDS             complement(763..1638)
                     /locus_tag="OUB02_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887254.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1642..2010)
                     /locus_tag="OUB02_003532"
     CDS             complement(1642..2010)
                     /locus_tag="OUB02_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8887255.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2193..2852)
                     /gene="deoC"
                     /locus_tag="OUB02_003533"
     CDS             complement(2193..2852)
                     /gene="deoC"
                     /locus_tag="OUB02_003533"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCT8887256.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIEDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2888..4075)
                     /gene="murJ"
                     /locus_tag="OUB02_003534"
     CDS             complement(2888..4075)
                     /gene="murJ"
                     /locus_tag="OUB02_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCT8887257.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(4152..>4729)
                     /locus_tag="OUB02_003535"
     CDS             complement(4152..>4729)
                     /locus_tag="OUB02_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT8887258.1"
                     /translation="IGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTI
                     GSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIRKRKLS
                     RLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWI
                     CPSCNETHDRDINASRNILKEGLRLITIQNK"
BASE COUNT         1562 a          812 c          469 g         1886 t
ORIGIN      
        1 tgcttttcta agtaataaaa ttataaatat tatgcaaata actataattc ctacctttgt
       61 tccaaataaa ataaaaggat tctgaaatga ataaataatt ttttctaaat acagtagcat
      121 tttatgcata tttttagttg caaaattaac tatataaatt accattaata tcggtaatcc
      181 tatcaatata gtaatactag taacttttct aactctatat gtaaaagctc caagtaacac
      241 tcctataaaa cctatacata tttcatttat acatatttgt acaaaataat ttaaataagt
      301 tagtggcata cttgatgttg aaggatctat cagtgctgat atagatgtcc aaatcatatc
      361 tctctttagt aaaactgata gttctatact gtaacctgtt ataatttcta taaataactt
      421 cgataaaaaa atcaaaacta tacctaaaat agaaatcaca aagcttaaaa taattaaatc
      481 tatgacaata gcttttatac aactttttct atctgctctt atacttaaag ctcctgaaaa
      541 gtctttattt gctatattta ctccatacac aaatagcagt atagttatat atgttaaaat
      601 aggattcaca atcgtggata aattagtttt agatgcttct ccaaaaatct tgagaataaa
      661 cactggcaaa atattcataa caattcccac aatcataaat ataattatat acaatttagt
      721 atttttatta aaaaatttta tatatggctt taattcattc aattacaatt cctccttctt
      781 agtcatgtta acaaacatat cttgtagccc tatgtatcct acatcaatat ttgaattttt
      841 aagtattttt tcatcacttt cactaaaatc accatagtaa aaataagcaa ctgcatttcc
      901 aaatgccttt ttaggtttta tattttttat acattctaat ttttctaact cttctttatt
      961 tccagtaata taatgagctt tttgttttat gacatcaatt tcttcatcta taataatttt
     1021 tccctcattt aatattatta catgctccag taaatcatct atttcatcaa tcaaatgagt
     1081 tgatattatt attgtccttg gattttttat ataactatct aaaattatgt tataaaactc
     1141 ttgtctattt actgcatcca aaccaattgt tggctcatca aatatggtga tagctgaatt
     1201 agaacaaata cctattatat ttgaaagtat agttttcata cctcttgaca gttgtttata
     1261 ctttttttta gtatttaaat caaaatattt gcataattct ttttctaagc ctttatcata
     1321 acttttataa aaactagaag aaaattcaaa tatctgacct actttaaaat ctggactata
     1381 aaattctttt tccctgacaa tacaaacttc ttccaaaact tttatatctt cttttgggtt
     1441 ctttcctaat atttctatct caccttcatt ttgaattaac tgatttacca ttatatttaa
     1501 taatgttgtt tttccagctc cattcttacc taataaacca tatatcttat ttttttcaaa
     1561 gcttagagac atattattaa aaatattttg cttcttaaaa ctgtgagata aattttctat
     1621 cttaatagaa ctactcatat attaatcctc cttgaaatta attataatat caaccaattc
     1681 ttctctattt atttctaatt tgttagcttc ttgaagtagg ttttttacaa aattatgttt
     1741 aaaattttct tttctagctt cttttataat agtttttgca ccatctgata caaacatccc
     1801 tatccccctc ttcttatata aaatattttt atcaaccaat atatttatac cttttaatac
     1861 tgtagctgga ttaattttat aaagctttga aagctctgtt gttgatggaa cctgttcttc
     1921 ttcttttatt ccatttgata gtatctcatc ttcaattgct cgtgctatct gaataaatat
     1981 aggttcttca ttatttaata ctaatttcat aggttcctcc tttctccatc agttagttac
     2041 tcaactaatt aactatataa cttataatat cttgaaaaat attttctgtc aatactaaat
     2101 aaaaaacaga gggaaaattc cctctgtttt ttgttcttta tatttataat taaattttaa
     2161 tttttgtaat cctatcccat aagttaaaaa ctttaataat ctgttgtatc ttcatttttt
     2221 aatattttac atattgagct agttcctaac ctttctgctc ctgcctctat aaatttttct
     2281 gcatcagaaa ttgatttaac tccccctgca gcttttattt ttacattttt tccaacatgc
     2341 tctttcataa gctttatatc ttctaaagta gctccacctg ttcccattcc agtagatgtt
     2401 tttataaaat ctgaacctga cattgttact atttcacaca tttttatttt ttcatcttca
     2461 tctaaataac atgtctctat aatgactttt aatactttat cttctatagc tttttttatt
     2521 tcttttattt cttttcctat attatcataa tctttatttt ttatatctga aatgtttaca
     2581 accatatcaa cttcatctgc tccattttca atagcatctt ttgcttcaaa tacttttgta
     2641 gcagtagttt gatatcccaa agggaaccct atcactgtac atatctttat tttacctttt
     2701 agatattctg atgctctttt cacatatgat ggtggtatgc aaactgatgc tacactcata
     2761 tctacagctt catcacataa aattttaata tcttcccatg tagttgtagc ttttaaaata
     2821 gtatggtcta ctgtttttaa tatatgtttc ataaaatcct cctaaattta taattaatat
     2881 aattagatta cttttttaaa tgcaattttc tctttgcaat attcagtatc atatcaacct
     2941 cttcaacttt aaaaatagtc attagtagtg tataaattcc acctccaact ataactgaaa
     3001 tagctagtga tacaaactca ttaaatgttc ctactcccaa aattccaaat ataaatttat
     3061 acgtaaagta tgataaaaca cccataatta aagatgcaac taccgatttc aaagttgctt
     3121 ttattatttt atcttgtcca aaatatccaa cttttctctt taggttcaag aagagtaaca
     3181 atatacatgc aattgatgat gaactagttg caagagcaag accaccatga gccattggtt
     3241 ttattaaaac taagtccaat actatattta cccctacaga tataatccca tttaccatag
     3301 gtgtttttgt gtcttgtaat gaataaaaaa cctttcccaa aatatctctt aagccaaatg
     3361 cagtcatacc aactgcataa aaaattaagg cagttgctgt catttgggtt gccctcgcat
     3421 caaaagcacc tctctcaaaa atgattctaa caacaggagt tgcaaaaaaa atagaagcaa
     3481 ctgaaatagg aatcatagaa attattatca tatttatact agatttaact gaactagtaa
     3541 acttcttttg attattttct gcacttaact tagataacat aggatacata acagatacta
     3601 ttgaagctgt aaatgtccct gtaacaaaac catttaatct atctgcataa gtaagtgctg
     3661 gaatgcttcc tttaactaat gttgaggcta aagttgtatc taccaatgag ttcacttgat
     3721 taacagcaac acctataaat actggtgaaa gtaatgctag taatttaata aggctatcat
     3781 ctttaaagtt taagtagtat aagtatttgt agttcgtttt tttaacaaaa aacatgtaaa
     3841 agagcagttg aactaccata gcaaccacag caccaattgg aagtatgtat ggtccaaata
     3901 tggtacttaa cattattgaa ataattatta ctatattata gggtatactt ccaaatccaa
     3961 ctacaataaa gttctcttta atttgtaaaa atgctgacat aacacttgta atacctatga
     4021 atattattcc tgttctgaac ctcccatgac taaagtcaca gggttcctgc ttcatagaat
     4081 tttgcatact aaaatatgtc ttactaattc tccacaggct atccccgtag ttcctacggt
     4141 tcttacatac gttatttatt ttgaattgtt attagtctta atccttcttt taatatattt
     4201 ctacttgcat ttatatctct atcgtgagtt tcattacaac taggacaaat ccattctctt
     4261 atatttaagt tcttaacttc ctcgttttta tacccacatt tattgcatat ttgtgaactt
     4321 gcaaagaatt tacctacctt tacaatttgt ctaccatgcc aattagcctt gtattctaat
     4381 tgacgaataa actctgacca tgatacatca gaaattaagc gagatagttt acgtttccta
     4441 atcatattct ttacttgtaa atcttctatg caaataatat cgttctcttt tattaattta
     4501 gtagatagtt tttgtagaaa atcctttctt tgattggcta tatgttcttg aagtcttgca
     4561 acttttaatc ttgctttatt tctatttaaa ctaccgattg tttttctcga taattctctt
     4621 tgtaatttag caagtttgtt taatgacttc ttaagatact taggattttc tataaattct
     4681 ccacaacttg agatacaaaa ttctttaatt cccaaatcta aacctatat
//
LOCUS       DAKBNN010000251         4717 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000251 DAKBNN010000000
VERSION     DAKBNN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.257"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            195..1346
                     /locus_tag="OUB02_003536"
     CDS             195..1346
                     /locus_tag="OUB02_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCT8887259.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1392..2567
                     /gene="thiI"
                     /locus_tag="OUB02_003537"
     CDS             1392..2567
                     /gene="thiI"
                     /locus_tag="OUB02_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCT8887260.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2723..3526
                     /locus_tag="OUB02_003538"
     CDS             2723..3526
                     /locus_tag="OUB02_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCT8887261.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3769..4410
                     /locus_tag="OUB02_003539"
     CDS             3769..4410
                     /locus_tag="OUB02_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCT8887262.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1884 a          447 c          915 g         1471 t
ORIGIN      
        1 ttttgtaggg tatagtataa gtatattata ttttactata aaaatttgta ataaagtgaa
       61 aaaactagct tttcatgaaa taatatttta taatttaaga ttgtcttttt aataaagatt
      121 aacaaaaaaa atactataat tagtggtata atagttagag ttgaagattt taaataaagg
      181 taggtgtaat taagttggaa atatacttag acaatagcgc aactacaaaa ccttatcaag
      241 aagttataga taaaatggtt tatgcactta atacggaata tggaaatcct tcttctgtcc
      301 atagaaaagg tgtagaagtt gaaaaagcta taaaagaagt aagacaagat atagctaaat
      361 ctctaggagc gaaagaaaag gagatttatt ttacatcagg tggtacagag tgtaataata
      421 ctataataag aggtattact agtttaaata aaaaaagaaa aaatcacata atatctacta
      481 acatagaaca tccttctgtt ttaaatacat taaaagattt agaagaagat ggttttgaag
      541 taacttattt agaggtcgga aaagatggga aaataaatat agaggattta aaaaatgcga
      601 taaagtcaac tacatgctta gtaagcatga tgcatgttaa caatgaaata ggaactatac
      661 agccaatagg tgaagttgga aagtatttga agggtttaaa agaaaaaatt tatttccatg
      721 ttgatgctat tcaatcatat ggaaaaatta actttagacc atctaaatac aatatagatt
      781 ttatgagtgt aagtgcacat aaatttcatg gtccaaaggg aattggattt atgtatataa
      841 aagaaaataa tcgccttaag cctatgttaa caggtggtgg tcaggaaatt ggaattaggt
      901 cgggaacaga aaatgtacct ggaatatatg gtcttggaga agctgttaga atactaaaca
      961 aagatttaga tgctgttatt tcgaaagtag ataacttaaa aaatatatta aaaaatgaaa
     1021 taattgataa tatagaagat ataaaaatca attctccaga agatggagta tgtcatatat
     1081 taaatgtatc atttagaggg acaaaaggag aagttttact tcattactta gagcaaaaag
     1141 gtatttatgt atcaactggc tctgcatgtt cttctaaaaa gaaaggtagt tacgtattaa
     1201 atgctatagg tcttacaaat gaagaaataa atggaacaat aagatttagt ttatctgata
     1261 tgaatacaga agaagaaatg ttagaagctg tgaaagtttt gaaagaatca atttgtgact
     1321 taaggtcaat aatgaaaaga aaataaagaa tactgtataa atagtgataa taaattagtt
     1381 taagaggtga aatggtgtat aatatattaa tagtaaagta tggagaaatt ggagtaaaag
     1441 gaaagaatag atatatattt gagaataggc ttataagaaa tataagaaat atgttaaaac
     1501 caattggaag atttaatgta tataaagagt atggaagaat atatgttgat ttagaagact
     1561 atgattatga agaagtaatt gaagaagtta gaaaagtatt tgggatagtg ggtgtgtgcc
     1621 ctggagtaag agctaaaaaa gattatgata cattaaaaga aatagcttta aaaatgttag
     1681 aagaaaaaat agaggctgga tataaaactt ttaaggtaga gtcaagaaga ggagataagt
     1741 ctttcggatt gacttctcaa gaaatgagta tggatatagg tggatactta ttatcaaagg
     1801 taggagatag gattaatgtt gatgtaagaa atccagaagt taaaataaag tgcgaataca
     1861 gagaatttca tactatggtc tatagtgata ctatacctgg atatggtgga ttgccacttg
     1921 gaacgaatgg tagagctatg tctcttttat caggaggtat agatagccca gtagctacat
     1981 ggatggttgc aaaaagaggt atggaagtag aggcagttca ctttcatagt tatccattta
     2041 caagtgaaag gtcacaggaa aaagttaagg atttagctaa aatactggca aaatactgtg
     2101 gtagagtaag gcttcataaa gttaatatct tagaaataca aaaagcaatt ggcgagaatt
     2161 gtaatgaaga agaagctact attctttcaa gaagatttat gatgaggata gcacagagac
     2221 tttctgaaaa aagacattgt gatgctttaa ttacaggtga aagtattggg caggttgcat
     2281 ctcaaactat acaaggactt acttgtacta atgccgttgt agacttacca gtgtttagac
     2341 cacttatagc aatggataaa tcagatatag ttgatatagc caaaaaaata gggacttttg
     2401 aaacatctat agtgcctgaa gaagattgtt gtagtgtatt ttctcctaga aagcccgtca
     2461 ctaaacctag attagagaaa atagaaaaat ctgaaacagc acttgacatt gaaaaattag
     2521 tacaggatgc tattgacaaa atagaggtag aggatattga gttttagcat acaagttatt
     2581 taattattaa attgaatatt gagtatatta gtagttctgt aagtatatta gtagttctgt
     2641 tgaaaggatt tatgtagaca atttattaat atgtagtatt taaaaataag tactataatt
     2701 ttatttaatg ggaatgatta ttatgaataa aaagggtttt acattaattg aattgttggt
     2761 agttatatct ataataggaa ttttagttat agtagctgtt ccagcgttat ttagaaatat
     2821 agaaaaaagt aaagcagtta catgtctttc taatagagaa aatataaaga ctcaaattgt
     2881 tattgcaatg gctgaggaat caagtaaaga caagaatgaa gtcataaaag aggtattaga
     2941 aaacaaagat ggtaagtact ttgaaacaga accaaagtgt aagtcaggtg gaatatattc
     3001 agcaacgttt gatgatggtt atgatggaat aactggaata gaaagcattg caaaagtgta
     3061 tgttacttgt acaaaacatc cagacggtgt tgaaatggct agggatgtgc atcaaagtat
     3121 gaaagatttg attgcatcat ttgcacaaga cccttctata ataccaggag cttcaaaggg
     3181 caatgatgat tttagaaaat atttattaga caataaatat aaaaatgggt ggcctacaat
     3241 tccagatgaa tttaaggcaa aatatggatt aagtaaggat acactatata tacaaccata
     3301 tgcatataat cctactaaat ctgatgctac tgtagttgta tttgcaaata ataagactgg
     3361 aggtaattgg tatacttccc tagtttacga ttatgatgaa ggtagatggt ataaaggtaa
     3421 aaatggtatt tctgttgcag gtaggtcatg ggatgttgac acagatagtg ttaagtctgt
     3481 aaaaacagag attcattcta aagagggatg gggtccttta aattaatata tcatgtttta
     3541 ttaatctggt attgattaaa ctatagaaaa gagcagtttc tatagatgac gaataccaga
     3601 ttttcattta tatatataat tgagatatat taaaagtgtt tatattttta agtcttcaca
     3661 tacaagctaa aaataattat aataatattg taaagaaatt taattataag attaattttc
     3721 tgaagattta gtatttgtac taagaaatac ataaaaaggg gaatagatat gaataataat
     3781 tttcaagaca accaaacaat aaatttaata caaagtcgta gaagtatacg aaaatttacg
     3841 acagaacaaa ttagtgatga gcaagtaaac acattattac actgtgcatt tgcagcacct
     3901 tcaggatgta ataaacagcc ttggcacata actgttgtgc aagaccagaa acttttaaaa
     3961 gagattagtg atgatacttt atctaggatt catgaggtga gtaatgttga gataaataaa
     4021 aactttaagt tattttatgg tgctccaacc gttttattta tttcttatga tgaaagtagt
     4081 tcatgggctc catatgatat aggaatactt acagggaata taacaacagc tgcacaagca
     4141 ctagggcttg gaagttgtat aataggaatg gttagaggtt tatttacacc tgttgaacaa
     4201 ggggatattg aaggtcttgt gagtgtattg gataaagaag atgtaaaaga atcagagtca
     4261 ataaagatga agtttgatac aaataaaaaa tatagagaac tacttgatat accagaaggg
     4321 tattctgtac catttggtat tgctgtaggt attccagatg gaaatttacc aaatgctaga
     4381 gaggtagttt ataaagtttc cagagtttag gatgaagtat tgcaagaaac aatcagttac
     4441 ttgaagaatt aaatcagtaa tatacaaata aattgatgga agaggaagct atttaaaaag
     4501 tagtttagat ttttcgttgt aaaataatta aataaagaat aaggtgataa gtattttatt
     4561 tattatctta ttctttttta tttttgtttt gtagaatgat aaaaataagg attaagtgta
     4621 ataaaataga aaacaagtat gttaatttta agtatatgct ttaaaattta tccaagttaa
     4681 aaatttaaga tatcttttca gtataatgga aaaaatg
//
LOCUS       DAKBNN010000252         4651 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000252 DAKBNN010000000
VERSION     DAKBNN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.258"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            121..1119
                     /locus_tag="OUB02_003540"
     CDS             121..1119
                     /locus_tag="OUB02_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HCT8887263.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1481..2008
                     /locus_tag="OUB02_003541"
     CDS             1481..2008
                     /locus_tag="OUB02_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HCT8887264.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2035..2631
                     /locus_tag="OUB02_003542"
     CDS             2035..2631
                     /locus_tag="OUB02_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HCT8887265.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFAKDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2710..4293)
                     /gene="asnB"
                     /locus_tag="OUB02_003543"
     CDS             complement(2710..4293)
                     /gene="asnB"
                     /locus_tag="OUB02_003543"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCT8887266.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKTEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         1640 a          717 c          657 g         1637 t
ORIGIN      
        1 tttcaaaact aaatttttaa ccttaaaaat tttgtataaa aaatataacc ttatatgcta
       61 catattattt ctaaatgaaa aattaccata ataatttaat aattacagga gggtattgat
      121 atgaaaatac tagtatttgg agcacgcgat tatgaagaac cagtaataaa aaaatggtct
      181 gaagaacata aggatgttca agtggatatt tatcctgaaa acatgactga agaaaatgta
      241 gttaaagcta aagggtatga tggtatatct atacaacaaa ctaactatat agataatcct
      301 tatatttatg aaactttaaa agatgctggg gttaaagtta tagcttcaag aactgcaggg
      361 gttgacatga tacattttga tttagttaat gaaaatggac ttatcgttac aaacgttcct
      421 tcttattcac ctaatgcaat agctgaatta gctgttactc aagctatgaa ccttttaaga
      481 aagactcctc tagtaaagaa aaaagtctgt gaaggtgatt accgttggat agctgaactt
      541 cttggaacag aagttagatc tattacagtt ggtgttatag gtacaggaaa aataggtgct
      601 acttctgcaa aattattcaa aggcctagga gctaatgtaa ttgcatttga ccaatatcca
      661 aatagtgatt taaatgatat attaacttac aaagattctt tagaagacct tctaaaagaa
      721 gctgacctta taacattaca tactccttta cttgaaggaa caaaacatat gataaataaa
      781 gatactctag ctataatgaa ggatggagct tacatagtaa atactggccg tggtggttta
      841 attaatacag gggatttaat agaagcacta gagtcaggaa aaattagagc tgctgccctt
      901 gatacatttg aaactgaagg attgttctta aacaaaaaaa tgaatcctgg agaattaact
      961 gacccagaaa taaataaact tctttctatg gaacaagtta tattcactca tcaccttggt
     1021 ttcttcacta gtacagcgat tgaaaatata gtttattcta gtttaagcag tgctgtagaa
     1081 gttataaaaa caggaactgc tactaataga gtaaattagt attttataaa tatatttttt
     1141 cattaattag ctattatcta acaaaatagc ctttaatttc ccaaatataa gagagtcagc
     1201 tcagtatata ttgagtagac tctcttatat tattttttat attaaacttg tactcttctt
     1261 attcatacca aattaaattt atattaactt attctaacta ctcctactct atacatttta
     1321 cctattttta cttttctaat tatatacatt tgaatgggta caatctgtat tttacaaaaa
     1381 aaacacctaa caaactatga aaattgtatg attaatatta atatatttat aagaaaacat
     1441 aacaattatg ttagctctgg atttttaggg agggagtata ttggaaaaaa tgaataaagt
     1501 tacttctaaa aaaactacct atattgttac ttcagcatta tttgcatcca taatatgcct
     1561 gacaatagca tatatattac atattccagt tggtggaaac aatggatatg ttcatatagg
     1621 agatgctttt atttatctag ctgcaactat tttgccaaca aactatgcta tagctgcttc
     1681 tgcaattggt gctggtctag ctgatttatc tacaggtgct gctatctggg taataccaac
     1741 tataatcata aaaccaatac ttgtattatt tttcacttct aaaagtgaca agataataaa
     1801 taagagaaat atagttgctt cggttgtagc aggtatagta ggattagttt tatacatgtt
     1861 tgctgaaggt ataattattg gaagttttac aagtgcattt gtaatgagct tacttggctt
     1921 attacagcca ataggaagtt ttattgtttt tataatctta ggtatggcac ttgataaact
     1981 tgattttaaa aaacgttatt ttaattaaga atttaaattt atggagggaa aattatgaat
     2041 atcttatata caattgaaaa tagtatctat gtaaatataa ctaatacatg tccatgtagt
     2101 tgtgtttttt gtataagaaa cgaaaaagat gaagttgcaa atagtggtag tttatggctg
     2161 gaacatgagc caagtgtaga tgaagtaaaa gaagccttca ataaatacaa tttagacgac
     2221 tatgatgaaa tcgtattttg tggatatggc gaaccattaa tgagaataaa tgaattgata
     2281 gaggtagcaa agtttataaa agaaaaaagt agcataaaga taagaataaa tacaaatggt
     2341 ctaagtgatt taatacataa caaaaaaaca gctattctgt taaaaaatgt tatagatgca
     2401 gtatcaataa gtcttaatgc cccaaataaa gaagcttata atagagttac tcaaccaaag
     2461 tttggagaaa aatcatttga ttatatgtta gattttgcaa aagattgcaa aaaatatata
     2521 aaagaagtag ctttttcagt agtagatgaa atatctccag aagaaataga agaatctaaa
     2581 caactggcaa aaaaacttga tataccatta agggttagac ataagaatta gcaagtaaaa
     2641 ttataaaaca ttaattataa aacattaaga atcacctgaa tggtctttca ggtgattctt
     2701 cctcttgtat taacatttta caaaatagat ttcataccac actaggaatg tgtaaatcat
     2761 ccaaatcttt ctccaattat cagagtttcc actaatgtag tcattaaaaa tctcattaat
     2821 ctcatctatg ttaaagaatt tatctgctat ctcactattg aatttatctc ggatgtcctt
     2881 attatattgg tcatcagcca tccatatacg aattggcaca ataaatccta atttatttct
     2941 aaaagcaatc tcttctggta atgtttttga tgcagctgta cgaaaggcaa tcttattctc
     3001 ttttcctttc actttatatt ctgatggtat tctggatgca atatcaaata ttcttgtatc
     3061 tgtaagaggc attctgactt ccaatccaac tgcagtactc attttatcta tattaaaata
     3121 aatatctcct tccaaccata tttgaatatc tacatttgac atgttagtaa gtgtatctag
     3181 ttcttctgtt tcttcataaa ttccctttac cacttcaata ggaagcacat caggattgta
     3241 tttcttaaga atttttcttt tctcaccctc attcataatg tttgtatttc caatataaaa
     3301 tgtcttcaaa ttctctccat aacactctgc cttatggtat atattatatc caccaaaaaa
     3361 ctcatctgct ccttctccag agtaacagat atttgtgttt tcagctgctg ccttgcatcc
     3421 aatagcaaaa acgatagcag atgcatctcc agttggttgc tccatatggt acattacata
     3481 aggtactgac gcaaaatact cttcaggtgt aattatatat ctcgaatttt tacacccaag
     3541 aatattagct gtctgttgtg ctaattttga ttcatcaaaa cgctcttcct cataaccaca
     3601 tgaacctgtt ttttctatat ctgacattgc caatatataa gatgaatcaa cacctcctga
     3661 taggaataac tctgcagttt cattctctga ttttacctct ggcataattt tctgaagtgt
     3721 tgtatgaatt tcatccgtcc attcctcaat agacttgctg tggtcaggat gaaattcagt
     3781 tttccaataa cgaccaatac atactgtttt attctgatag acaaggtaat gtcctggcat
     3841 aagcttcttt actccacgga agaatgtatt ttcacctgct acatatgtga aagacatata
     3901 aatctgtaac atgtcttcat ttaactcttt cacaaatcct ggttgctcca taatcttacg
     3961 aatagatgaa ccatagagaa gcttcccatt ttcagtctca taataataga aaggttttac
     4021 tccaaactgg tctcttaggc agaagagttt cttttccaca gtatcccata atgcaaaagc
     4081 aaacattcca tgcatgtgat ttgccatatc atatccccat gtcttatatg cttccactaa
     4141 aattgctcgt tcacgctcat ctctacataa tgaagaatcg attccaagtt cttcacagag
     4201 aactttccag tttcgaatat gtccattgta ttctagaatt tttatcatat atgtgtcttt
     4261 tcgtgttaaa tacttttctt ctaaatttat catatgtgta tcctttcgta ttaaaacatt
     4321 taacttctaa atttattaaa tgtttatata tatttattca cttataatca ctgaaaaatc
     4381 gacttttttc aaagtaaatt ctttttgaat tagtactaat ctgctcttta ttaatggcat
     4441 caatatgatt atcaagtatt atcagtgtta taacactgaa ttttttattc ttcataaata
     4501 ttgctccctt tatattacca actaaatata atatatctta tgttacttac atattcttta
     4561 agaaattctg aagattttat aaagtttttt gtattataat caattaaaaa taaaattcta
     4621 gttataaagc atattttcca ttaaacaaaa a
//
LOCUS       DAKBNN010000253         4645 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000253 DAKBNN010000000
VERSION     DAKBNN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.259"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            88..1443
                     /locus_tag="OUB02_003544"
     CDS             88..1443
                     /locus_tag="OUB02_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8887267.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFVICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLAFLKKNFSTLDSNLNKV
                     A"
     gene            complement(1561..2229)
                     /locus_tag="OUB02_003545"
     CDS             complement(1561..2229)
                     /locus_tag="OUB02_003545"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HCT8887268.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVENIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(2296..2724)
                     /locus_tag="OUB02_003546"
     CDS             complement(2296..2724)
                     /locus_tag="OUB02_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCT8887269.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            2831..3424
                     /locus_tag="OUB02_003547"
     CDS             2831..3424
                     /locus_tag="OUB02_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCT8887270.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNINFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            3543..4478
                     /locus_tag="OUB02_003548"
     CDS             3543..4478
                     /locus_tag="OUB02_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCT8887271.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMVETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
BASE COUNT         1595 a          646 c          622 g         1782 t
ORIGIN      
        1 taccatagtt tatcatttat attagaaata aaattattaa ttaatagttt cgtaatttta
       61 taatagattt acagggagtg ataataaatg gatacaaaaa tatctataaa cgaaaacatg
      121 agtctaggta aaagattttt taaataccta gcaccatctg tagttgcaat gtgggttttc
      181 tcactatata caatggttga tggaatattt gtaagtaaag gagttggaga attagcttta
      241 gctgctgtca atatatcaat gccttttatt aattttatat ttgcagtttc tctattattt
      301 tcaactggtg catctaccat aattgcaatc tatctgggga aaaaagatat taaatctgct
      361 aacgaagtct tttcatttaa cttagtatca ataattatat tatcaataat tatattagct
      421 ataacttttt ttaatttaga tagattagca ctatttttag gagctacaga atctacaatt
      481 ggtatggtta aagattattt aggaataata atatttttta acggattctt tatagtgtct
      541 tattctttag aagttataat aaaaactgat ggctttccaa tattagctac tgttggtgta
      601 ataatatctg ctcttacaaa catcatactt gattacttgt ttgtaattga atttggatgg
      661 ggtgtaaaag gtgctggtat tgcaactggt ttgtctcaag tcttttctac tattttcttc
      721 ttaatacatt ttttaagaaa aaactctact ttgaattttt caaaatttag aattgatttt
      781 aagacactta gaaaaattgt atttataggt tttcctgatt caactactga attaagttgt
      841 ggtatagttg ttttactttt taacttaagt cttactaaat atattggaga aaatgctctt
      901 atttattata gtgtcataaa ttatataaat acattagtac ttatgactat gatgggtata
      961 actcaaggta tgcaaccact tactagcttt tattatggtg caggaaatat agataatgta
     1021 aaaaaattat taaaaatggg gataaaagcc acaataatag catcagttgc tgtatttgta
     1081 atttgtatgg ctttttcagg accaatagtc tctttattta tacatccaga agaaacaatg
     1141 ctattcaatg aaggagttcg tgtatttaaa atattctcta tatctttctt attggtagga
     1201 attaatgtaa taatttctgg attctttgta tcagtagaaa aaccatcaat ttctactgtt
     1261 atatctcttg gtagagggct tgtaatagtt gttttaagtc taatttcaat gatactaata
     1321 tttggtggac aaggaatatg gatgactact attgtttctg agtttatatg tcttatttta
     1381 tcattagctt ttctaaaaaa gaatttttct acattagact ctaatctaaa taaggttgct
     1441 tagatgataa gagtgacaat atatgaaaat atctaaataa aaaataccta ttatacaatc
     1501 ttacaaatat tagacctttg caaaaattag gattatataa taggtatttt atttatgtaa
     1561 ttattttatt tctaaatatc catagccatc ttttattaaa gttcctttta cttcatattc
     1621 tacaggtttt ttaaatattg gaccatctac gacaaatgta tgatactctc catgttctcc
     1681 acatgggtct gcaccagcat tttttatatt ctccacaagt tctctagtta atttttttcc
     1741 taaaaattcc tctcctaagt gctttaagtt tacttttgtt acaacactac aaaatccact
     1801 gtctataaat tcatatacta aatcctctct actctcttgc cataatggta aatctacttt
     1861 cataccaact gcttcacatc tatcagtatc ccattttcta tgactttcta tatctatatc
     1921 tccatatgca catatcgttg ctcccatgtt tttagctttt attaaagccc tctcaaagct
     1981 ctcttcatat ccttctacgc cacactcagc aactaataaa ggtaatccta tgttgctact
     2041 tacttgctta agcatattat attctaaatt atgtgtccat gactttccat gttcttcact
     2101 tatagttgtc aagagtgcca caggcttata tccttttttc aacattcgat ttaatgctaa
     2161 tatactatcc ttaccaccac taaatgacat tacaaatttt tcatcagcca attttaacac
     2221 ctctttcatc taactttgtt ttattaacat aattatacca aatttttgag ttgcatagca
     2281 aattatatta aattgttaat tcactataaa gaatgtccct aatattaaaa gtataacccc
     2341 tatgtatttg tataaatgca ttgtttcttg tcccaaaatt acgctatcaa taaaaaatcc
     2401 aactaaaact tgggatacaa gagttaatat aagtgtattt gatactccta aatttggaat
     2461 tgcctttact gtaaaatata ttattagacc tccaaatact cctcctaata acaacttata
     2521 attaaaacca tttatagata ctagacactt taagtcacct gtaactatta tacttgccaa
     2581 aaaaaatatt gctcctacag ttaagcttat aaaggtggcc actaatgctg ttgtactttt
     2641 tcctagctct ccatttataa aagcttcaag agctgtagaa ccacctgcta ataatgcaaa
     2701 tattattgcc cataattttg tcatggacaa acctccttct atataattct attaatagaa
     2761 gtttatgata caacctatta attaattact aagtatagtt taatttttat acactaggct
     2821 ttatttggat atgaatttct gttataattg ttctatcgat tttggaggtg attttatgta
     2881 cacaaatttt aatatcaatt ttaataactt taataaaaaa gaaaatgcta ttaaatttat
     2941 gttaatggga gtattactca ttatattagg attattatgc cttacattta agacattagg
     3001 tataaaatta atttcttgga cttttggtat tgcactttta ttctttgcat atttaaattt
     3061 aaaaaatatt aatgagttaa aaagatatgc tactaaagaa gaaattaaac cctctattaa
     3121 tatccaatgg gttctaataa ttgcatgtat attacttttt gtatttcctc aaaaaataca
     3181 aagcatattt tcattatttt taggatttta tttaatcttt aatcaactag tagcacttgt
     3241 aaatagtaag aataatcctt attctaaatt tacaacttgg aatatagtta agattctatt
     3301 tggtatatgc cttatattat ctccactttt tttatctaga tttatagtat cgataatgtc
     3361 ttttttcata atattatttg gactagttct atttttctct ggaaatacag cgagaaaata
     3421 ttaattattt tctcgctgta tttttatatt acttaaaata gaatttgttt aagttatcta
     3481 tttgtggtaa tatgtttttt gtacaagctc agcgtacaat tttattagaa ataggtgatt
     3541 ttttggataa atttaaatat gcttttgata ataaaagata tcatacttgg aattactatc
     3601 ttagaaatac ttttggagaa aaagttttta aggtatctat aaatgcagga tttacttgcc
     3661 caaacattga tggaagtctt ggttatggcg gatgtaccta ttgtagcaaa gaaggctcag
     3721 gtgattttgc tggaaaccct aaagataatc ttatcagtca attttataat ataaaagaaa
     3781 tgatgttaaa aaaatggcct catgcaaagt atattggcta ctttcaagcc tatacaaata
     3841 cgtatgctcc tcttgaagtt ttaaaagaaa aatatgagac tatattagaa cttgaagatg
     3901 tcataggtct ttcaatatcc acaagacctg actgtttgcc agatgatgtt gtagaatact
     3961 tatctgaact aaataaaaga accaatttat gggttgaact tggacttcaa acaattcatg
     4021 attctacatc taaaattata aatagaggtc atgattataa aacatttgtt gaaggtgttg
     4081 aaaaactaaa gtctaaaaat ataaaggttg tcgtacacat tataaatggt cttcctggtg
     4141 aagattataa tatgatggtg gaaacagcaa aggcagttgg taaaatgggt gtagacggta
     4201 taaaaatcca tctattgcat gttataaaag atactcctat ggaaaagatg ttacaaaatg
     4261 gtatgttaac tcttatggaa caagatgaat atatcaatct tgtctgtgac caattagaaa
     4321 tacttcctga aactatgata atccatagac tcactggtga tggtaagagg gatgaactag
     4381 taggtcctat atggagccta aaaaaatggg aaattttaaa tcaaattgat gatactttaa
     4441 aggctagaaa ttcttatcag ggctgtaagt ttgtttaata caaaataata actatagaaa
     4501 tggttatata aatatagtcc tttctatagt tatttagtat ttacaaaaca aaatattaat
     4561 ttgtacattt atacaatata taataaaaaa tagatagtct aaactgtatc tacttatatt
     4621 ttatttgtac tgtccacttt attct
//
LOCUS       DAKBNN010000254         4643 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000254 DAKBNN010000000
VERSION     DAKBNN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.260"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            271..1188
                     /locus_tag="OUB02_003549"
     CDS             271..1188
                     /locus_tag="OUB02_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCT8887272.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGNIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1317..2246)
                     /gene="pfkB"
                     /locus_tag="OUB02_003550"
     CDS             complement(1317..2246)
                     /gene="pfkB"
                     /locus_tag="OUB02_003550"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCT8887273.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            2513..3238
                     /locus_tag="OUB02_003551"
     CDS             2513..3238
                     /locus_tag="OUB02_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8887274.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3321..4475
                     /gene="nagA"
                     /locus_tag="OUB02_003552"
     CDS             3321..4475
                     /gene="nagA"
                     /locus_tag="OUB02_003552"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCT8887275.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKNPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICMYKVNK"
BASE COUNT         1732 a          626 c          662 g         1623 t
ORIGIN      
        1 gtatttttat tttacgataa aaacttgttc agaccaaagt ggtaattacc actaatccaa
       61 atataaatta atttgaattt tttgtcaata cataaaatat ttatatcaat caattttatg
      121 gatatcctac aaaattactt tatcaatatt tcttatttcg ctaatttact tatataagct
      181 ttaaatttat tttttaatac aaagtttaca aatttatgtg tctgaattat aatcaattct
      241 gtaaatataa aaaccttagg agaaagtatt atgaaaaata taaaaatggg aatgataggt
      301 cttggctcca tagctcaaaa ggcatattta cctatattaa ctaagtctga gagatttgag
      361 tttgtaggag cttttactcc aaataaagtt aaaagggaaa aagtctgtag tgattataga
      421 atcatgccat tcgattctat tgaatcccta gctaaaaaat gtgattgtat ttttctacat
      481 agctctacag aaacacacta cgaaattata aaaatattat taaatttagg tgttcatgta
      541 tatgtagaca aacctcttgc ttcaacagta agtcaaggag aagaacttat cgaattaagt
      601 acgaaaaaaa acttaaattt aatggttgga ttcaaccgta gattctgtcc tatgtacaaa
      661 gaaattaaaa ataatgctac agaaattgta tctatcaata tatgtaaaca tggattaaat
      721 tccttaagaa atgttaaatt cgacagtact cttatagatg actatataca tgttattgac
      781 actgctttat ggttagcaaa tgaagatgtt gaaattagtg gagaggattt atttttaaca
      841 gacaataaga atcttatatt tgtaagtcat aaacttaaag gtaaaaattt ctctataaac
      901 acctctatgc atagagattc tggaacaaag ttagaacaag ttgaaattct ttcaaagggt
      961 aatattcaaa gagttaagaa tcttaatgta ctggaaatag aagaaggtgg aaacctaact
     1021 ttaaagcaaa gtggtgcatg ggtaaacata ttaaaacaaa agggttttga ggatatatca
     1081 aatcatttta ttgattgtat agaaaataat ataaaaccag ctattaatgg agaagagtgt
     1141 atcaaggctc aaagattact agagaaaatt ataaattcag taaaatagat atttattata
     1201 aaaataggtt aaaaatgaat ttttctaacc tatttttata taagactata tcttttatat
     1261 taaaattaaa atctattaca acaacattaa atttattata ttcccattaa atgtatttaa
     1321 cttaactttt ttacttcaac taaatcttga tatttatcaa tttctaccaa atctacaaat
     1381 ccagtttcca tatttaaagc attacttata ccacaagcat ttgcaaattt aagtaaattt
     1441 tctattttat aaccccttaa aatacctaca ctaaatccag ccactgtgct atctccacta
     1501 cctacagtat ttatacactt tacaactggt acttttacat catatacatt atcttcgttt
     1561 aaatacacca taccatcttt tcctaaagat atacatacat tctctgcacc catttcaata
     1621 agtttttttc ctgacaagat aatttcttct cttgattcta tattcattcc taataaatgt
     1681 cttatttcat ctatatttgg ctttattaaa tatggttttg attttaaagc tatttttaat
     1741 gcttctcctg atgtatctaa tataaacttt atattatttg ctctacatat atttcctatc
     1801 ttttcataat agtctatcgg catattctca caataactac cagaagctac cagtatttta
     1861 gtatttttta gtacttcctc tatatcattt tcaaatctct ctaaatcgct tttttctact
     1921 attggtcctt tttctaaaaa ttctgtacta actttattgt cctcaattat gtttaagcaa
     1981 tttctagttt cctgctctat ttttgtaaat ttattttgta tatctagttt tctaagttca
     2041 ttttttataa attctccatt aaatccgcct agaaatccca tagctaaagg attttctttt
     2101 aaaattttac ataccttagc tacgtttaat cctttacctc cagcagttgc attcttactt
     2161 actacccttt gcacctcacc tatgtttata ttattcactc tatacattct atctatagat
     2221 gggttgaatg ttattactgt aatcatacta gattctcctc attattaatt tttacttttc
     2281 taaacaataa attaatctgt aaaataaaaa atacctcata taatatatcc aaagtggtta
     2341 ttaccaattt gaatataaat tattttactt gttttgtcaa tgtaccataa ttttataata
     2401 aattaatagc cttacttatt gaattttgat taaactttat tcattgacat aaataattct
     2461 atattctata atcaaattgt ggtaataacc acttagaaag gactatattt ttatgataaa
     2521 aagaaatgat aaaagaccaa tatatgacca gcttgttgag atattaagaa ctaagattga
     2581 aaatgagatg gaaccaaacg atagaatgct ttccgaaaga aaaatttgtg atgaatatgg
     2641 agtaagtaga accacagtta gactagctat ggctgagtta gagcatatgg gatatatata
     2701 caaaagacat ggaaaaggaa cttttgttgc tgcactaagt caaaattctc aaaacttaat
     2761 ggaaagttat agctttacag attatatgag aacacaagga aaaaagccag gtacaaaggt
     2821 attatcattt gaggtagtag aaagtacaaa gtatttctct gaaaaattag gtataactcc
     2881 tggtgaaaaa atgataaaaa taatgcgttt aagacttgct gatgaccttc ctatgatgct
     2941 tgaaagaaca tatcttccta tgaaagaatt tataggattg acaaaagaaa aaatcgaaca
     3001 aaaaccttta tatgaaatat ttagagaaga ttatggcgag attattaaag ttgctgatga
     3061 agaattttca gctggaataa tctctgataa ggaagctaag ttattggaag taccaggaga
     3121 ttcaccttgt ctgaaattac ttcgtaccac atataatgat gacaatatag ttattgaatt
     3181 tactttgagt gtcgctagaa gcgataaatt tgtatataga gtaagacatg taagatagta
     3241 agttaaataa ctttcaagaa aacagaatga tattataagc gtcaatgcca caatgatagt
     3301 ataattataa ggaggttatt ttgaaaagtt ttatttatgc agataaattt tttatgaaac
     3361 atggaatcaa agaaaaaggt taccttagta taattaatgg caaattcggt gaatttcaac
     3421 aagaaagacc taatgttact gataatgtta tagatttttc aggtaaatat attgctcctg
     3481 gcttagttga tactcacatt cacggtcttt taggtgcaga tgtcatggac aatacatttg
     3541 aagcaataaa cacaatttca aaaggcttat taaaatatgg agtaacatct ttcctaccta
     3601 caactttaac tgattctgta gatacactaa gtgattctgt agaaaatata agcaatagct
     3661 tcaatcgagt tgagggtgca aaaatacaag ggatttattt agaaggtcca ttttttacag
     3721 aaaaatataa gggcgctcaa aatgaaaaat attttaaaaa ccctgacatc aatatattaa
     3781 aattatggca aaaaaaatcg aaaaatttaa taagaaaaat agcaattgct ccagaacgta
     3841 aaggttcaat agaattaaca agatatgcaa ctaaaaataa tatatcagtt tctcttggtc
     3901 atagttcagc tacatttgag caagttaaac aagtcgttaa ttcaggtgct aaagtgtttg
     3961 tacatacata taatgggatg agtcctttaa atcatagaga acctggaatg gttggggctg
     4021 ctatggtact aaaagatact tatgctgaat taatttgtga tggtcatcat gtaagcccaa
     4081 tagctgttaa aataatgatg gatgctaagt ctagtaataa catagcttta attacagatt
     4141 gtatgagagc tggtgctatg gaggatggaa aatatactct tggtaagttt aatgtaaatg
     4201 taaaaaatag tgttgcaaga ttaaatagtg ggagtttagc tggaagtgta ttaactatgg
     4261 acaaggctgt gagaaatata gttgattgga acatagccac tttagaagat gcaataaaaa
     4321 tggctacata tatccctgct ttaagttgta atattgataa tgtttgtggc tccatacatc
     4381 aaggtatgga ggcagatttt atagtaacag ataaggactt taatatatac gaaacttata
     4441 taagtggtat atgtatgtac aaagttaata aatagattca atataaagtt atagaggctt
     4501 cctaaaaata gagataagcc tctttttcat tgttaaaatg atgaaaatac atttgatttc
     4561 atacaaaaag agtgcctcat aaactaaaag taggaacctt cctttatata gtgatattta
     4621 atatttcaat tatcactact ttt
//
LOCUS       DAKBNN010000255         4607 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000255 DAKBNN010000000
VERSION     DAKBNN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.261"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            117..305
                     /locus_tag="OUB02_003553"
     CDS             117..305
                     /locus_tag="OUB02_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCT8887276.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(465..617)
                     /locus_tag="OUB02_003554"
     CDS             complement(465..617)
                     /locus_tag="OUB02_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HCT8887277.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(715..1191)
                     /locus_tag="OUB02_003555"
     CDS             complement(715..1191)
                     /locus_tag="OUB02_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCT8887278.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1410..2171
                     /locus_tag="OUB02_003556"
     CDS             1410..2171
                     /locus_tag="OUB02_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8887279.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2431..4359)
                     /locus_tag="OUB02_003557"
     CDS             complement(2431..4359)
                     /locus_tag="OUB02_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCT8887280.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
BASE COUNT         1718 a          627 c          531 g         1731 t
ORIGIN      
        1 attcttcaat atgtgtaagg caaatatata tttctattgc aaatttttgt tatttatgat
       61 aatataaata tgtttttagt ctttaaatag ttatcatttt aaggaggtgt gcgtagatgg
      121 caaaagtatg tagcgtatgt ggtaaaggta aggtttctgg aaaccaagta tcacactcaa
      181 ataaacataa taaaagaaca tggtcagcta acttaagaag cgtaagagcg attatagatg
      241 gagctcctaa gagagtaaaa gtttgtacta gatgtttacg ttctggtaaa attgaaagag
      301 cttagtaacc acgtttaaaa gcctacttcc gtaagtaggc tttttaattt aatcattttt
      361 actctttctc ataaatttta ggattatact tgaaatccat tttggtaatt ttattgttac
      421 tactttcatc aatactcacc ccacacttta tagttaaaac tattttaaca aaaaaataat
      481 aatgctccac atccaataag tattaatgcg gtaagtgctg accaaatcca gccaggaagc
      541 aacattgaca gaattacagc cattcctgtt gctagacaaa gtattcctag caatacctta
      601 gacttgttat ttctcatgga acacctccac atagaacctg tatctataaa tattttcata
      661 ttcaagttta tgcgacagtt ttccatttta gaccaataaa cacattatgt aattttaatt
      721 tttcatacta gaattattac tgaactccga tatagattct acaatatcca aaattccttt
      781 ttttctactt aacctttcag gtccactata taaaacaact ggtataactt taggaagttt
      841 aaaattttta tcttctattt cttcaaggct ttttttcata ataaaacttt tccaaatttg
      901 ttctctatac gatttcaatc ttgggacaat ttcagtatcc atttctgttt gaaattctaa
      961 cactatataa tgtgatactt tgccgctttt agaaaatacc ttataaatta tatcaacttc
     1021 ttttccttta tattcacata taaaagatgt ttctaactct atatttttaa gagttaattc
     1081 ttttgctatt gagacacttg taaaattttg aatcaaactt aaaaaacttt ctttatctga
     1141 atacattctt ctgtactctt ctcgtttttc attgcttctt tttaaattca tacagttcct
     1201 cctagactat agaccctcta ttttttagta aagatagtct ataaatttta ttttttaatt
     1261 ttcattagtg agtattgaca gttgtgtgta aaatatccag taaagatatt tttttatatt
     1321 tttattaaaa gatataaatt tttatatttt aatgtattga catttacgtt gcgtaaaagt
     1381 gtatatttaa tttataggag gtactactta tggaatatac agttcaaaaa ttaagtaaac
     1441 tagctggtat aagtactaga acacttagat attatgatga aataggtctt cttaaaccac
     1501 ttaaaataaa ttcttcaggt tatcgtatat atggtcaaaa tgaagttaat aaacttcaac
     1561 aaatactttt ttatagagaa ttaggaatta acttagaaaa tataaaaaac attataaatt
     1621 caccaaactt cgatagttta agtgcgttaa aagaacacca taataaatta ctagccaaaa
     1681 gaaagcaaat tgatttacta atagaaaatg taactaaaac tatagctctg aaggagggaa
     1741 aatacactat gacagatata gaaaaatttg aaggttttaa agaaaagatg attgatgaaa
     1801 atgaaaaaaa ttatggaact gaaattagag agaaatatgg taaagatatt atagatacat
     1861 caaacaaaaa acttaaaaat atgtctaagc aagattatga aaattggcaa aacctaaata
     1921 tcgaaataat tagtaaactt aaaaaagctt ttaaaactgg tgatgcctct agtgaactgg
     1981 ctcaggaagt tgcaaaatta catcataaat ggttgagtta cacttggaac acatactcta
     2041 aagaagctca ttcctctcta gcacagatgt atgtatatga tgaacgtttt acatcatatt
     2101 atgatagaga acaacctgga ttagctaagt ttttaaggga tgctatagta atatatacaa
     2161 gagataaata atataaaata ataaaggtgg tgtctcaaaa tgaacttttt agttcatgag
     2221 acactctttt ttgtatgaaa tcagatatat ttttatcatt ttatcaatga aaaacaggtt
     2281 tctctctcta ttttgagaca gaatctttat tgtataataa ttataatcaa tacaaaataa
     2341 taacaaatta tacaaaactt acttatacta tttatgctaa aaacaaaaaa tattttatta
     2401 aagtttttaa aacttatgta atataaaaat ctatactata gacttgatta tggaaagaaa
     2461 attatcaaaa tcattacaat tagatatact atctctaata tcattgtcat gaattatctt
     2521 taaaaggata tcataaatgg agtttatttt tatataatca tctttattta tagccaacat
     2581 catgattaat ttaacagtag aatcttcact ccaatatatt ggttcatcca atatggaaat
     2641 acaaatctta gttcttatag agcttaattc cataggatga ggtatagcta aaaaatcatc
     2701 tatattggta gaagataaac tttctctgta aaatacagat tcagcaaatg atggaaatac
     2761 aatttcactt tgagttaatt tgttgcacat caactttata agctcatctt tatctttgat
     2821 tttaggatta acaataaata aatttttatc aaagaaattg tccaaaagat tactataaat
     2881 atgggaatta ttagtgattg acttagatat attctctata tcttttttta atagttttaa
     2941 atccactaat acaataggaa tactatcatg atttaaagga attgtcgata ttattatatc
     3001 tacatctgac aattcactag ctaaaaattg attaaatgat agacattgaa gtatatttat
     3061 tttatcatga aaaactttgt ttaattgaac ctctaataac ctacttgaac cataccctga
     3121 accacatact aatactacgt ttttacactt aatattactt tgaaaaaatc tctctattcc
     3181 agcacctata tgcagggata catatccaat ttcatcttct gttaaagagt aaatactatt
     3241 tttaaaaact ttttctatag catttaaagt tatttcaaat gctaaaggat aattactctt
     3301 tattgtattt ataaggggat tcaccttatt taaattataa gatttagaat tgagaattga
     3361 tttaaagtgc agtactaaat catcaaataa tacactatca tttctaaggt caaaagtata
     3421 ttgattgttg attacttcta aaatttcttc aacatactct ataattttag tatctaccct
     3481 attggaaacg ttatccaaaa gagaactttt agttataaaa tgattatata gataaactct
     3541 gtctgccttt gaaataatta tagtatattt gctctcaata taattaaata tattttcaac
     3601 agttgtattc gattgaaaat ctgttaaaac aacatctaaa acattatcta agcagtaacc
     3661 atcaagtatt ctacttacag taatagctaa atggataata aagtttttta gattaaaatc
     3721 taggaaattg atattaaatt tattaatttc acttaacact accctttgaa gctctattaa
     3781 atctacatta ttaaataatt ctatttctct atctgtaatt ccaattatat agttttcaaa
     3841 attcctattt atcatattat tagaaataca tcttcgaata tctatttcct tcccttcaac
     3901 tttaactcca taatatggct tagaaactaa tattaaatta tactttgaca aaataggctt
     3961 tattcttttt atgtcattta gtattgtaaa tttactcaca tacaagctat tagctaaatc
     4021 ttctagtttt atatatgtat tgttatagag aatatatttt aaaatatatt gatttctttc
     4081 tataggagaa tctggtattt cacttgaatc cataatatca tcagatatta atgctagata
     4141 ctgttggtat aatgctaaat taagaatatc tatataatat ccttctcctt tttttatttt
     4201 aataatagca ccatttttct ctaaagttcc atttatagaa gtaatatcat ttcttatagt
     4261 tctctcactc acttttaaat gattacttaa agttttacaa gtaattttat catcactttc
     4321 atttaataat ttaaacaacg tgagcaatct cttgtgcata aatatccccc cttatagaat
     4381 ttcttctaaa ttatactatt tacactaata taatacaatt actctaataa aattgtatta
     4441 taataattta tttccacttc tatatggaaa ttagttatta tcataatata aatttccatg
     4501 tcgaagtgga aattagttat atttaattta attaggataa tgattacaat ataaatataa
     4561 gttaagacat tatgaatgga gtggaaaaat atatggtact acaagaa
//
LOCUS       DAKBNN010000256         4589 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000256 DAKBNN010000000
VERSION     DAKBNN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.262"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(193..570)
                     /locus_tag="OUB02_003558"
     CDS             complement(193..570)
                     /locus_tag="OUB02_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8887281.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            911..2023
                     /locus_tag="OUB02_003559"
     CDS             911..2023
                     /locus_tag="OUB02_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCT8887282.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(2231..2707)
                     /locus_tag="OUB02_003560"
     CDS             complement(2231..2707)
                     /locus_tag="OUB02_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887283.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2906..4531)
                     /locus_tag="OUB02_003561"
     CDS             complement(2906..4531)
                     /locus_tag="OUB02_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8887284.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1625 a          729 c          481 g         1754 t
ORIGIN      
        1 aatattaatg acaaacccaa tataaattaa attcaaacag gtttgatttt acatcaaact
       61 tattgtatta atattaaata acaagacaaa aatatttata aaaatcacaa attttacata
      121 aaactttata tttgattcat ttaactaatt attgtaaaaa ctatttattt tatgataatt
      181 aactacacca aattattcat cttttttaat atccttaacc catttttcaa cagagttcaa
      241 ttcttcttca tttaccccct ttttgaacaa ggatttcata aaactctcaa tagaacttcc
      301 atgcatatta tctaaaaaag tttttgtttc aaaatgtaaa tactcttctt ctccaataag
      361 aactgtataa tgtgtatgtc tatcaatttt tcttgcactt aagaattgct tatttactaa
      421 tcttgataaa agtgttagtg ttgtagtttg cttccaatta tatagttttt ccatagcttc
      481 aatgacatct tttgatgcta ctgtaaaatc tgaactccaa ataaatttca tgacttttaa
      541 ttctgctggt ggtatcttag taatagtcat atgattttac actcctctca aacttctaca
      601 taattaaata ctatattaat aaacttttcg agtcaaaggt tttatttaaa atatctcatc
      661 caaatttatt aatacaaaaa atttatattt ttattcaaaa ttaaacacac aatctattaa
      721 tataaagata aatatctatt ctataagtat aacagattat caaattaaaa ttctaatatt
      781 tgacataata tatacatttt ttaaataaat atatacaatg tattaaaatg tatgcaactc
      841 tatttcaaaa ttattttgtg atatatatat gttataataa atgtataaaa ttttgattgg
      901 tgggttatat atgaatgcat ttattagaaa aagaaataaa aactatgttg tttacctgga
      961 atttgaagat gaaaagacag gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa
     1021 aagggaagca agtaaaagac taaatgaggt aaaagaaagc ttatataatg atggtttttt
     1081 agtaccaagc gaaattacag tgtcagaatt tctattagat tttctaaata aatatagtga
     1141 aaatatatct ttagctacat ataataatta tgttagtatt tgtaagaatt acattaatcc
     1201 atctattgga aaatataaaa tacaagactt acatccagtt cacatacaaa actacataga
     1261 taaactatct tataaactaa atcctcaatc aattaagatt catataaata tattgaaact
     1321 tgcaataaaa agagcttata gactcaaatt aataaaagaa aatgttatgg atagtataga
     1381 ggctccaaga tataaaaaat ttaaaaatga gatttatgat agagaacaaa tgataaaatt
     1441 attagattta gcaaaaaata cagaaatgga attacctatt aatttggcta ttggattagg
     1501 tcttagaatt tcagaagttt taggtctaac ttgggataat attgattttg aagaaaatac
     1561 aataacagta aacaagataa cttcccgaat taatggttct gttattctca aagagccaaa
     1621 gacagaaagc tctgttagaa aaatatcttc tcccaaagaa ttaatgtctt tattaaaaga
     1681 atataaaata aaacagaata aaaatttatt aaaatcttcc attagaaata ataataattt
     1741 gttgtttttc aataagaaat gtgaacctat tgctgaagat gttatgagta aaaaatttaa
     1801 aagattttta gaaaagaatg aattacctca tattagattc catgatttaa gacactcaca
     1861 tgttacttta cttataaatt ctaaagtacc tataaaggtt atatccgaga gagtaggaca
     1921 ctcaaatata agtactactc taagcgttta ttctcatgta cttaaagaaa tggataaaga
     1981 ggcttctgac aaaatttctg aaagtttatt taatgctaat taatattaat ttatcttagc
     2041 tttaaattag cttagcttat tgagatagat tcattaatat taaatgtatc tatctcaaat
     2101 taataactac ttaaataatc tgttttattt taatttatcg cctcagttat tatacttttt
     2161 tacgaaaaat tatactttac aataaaaaag atattccctt atagaaaata tctttttaaa
     2221 tactatatta ttatatactt gctaacattg gaatatctaa catcttcttc attacatcat
     2281 ctactttttt tgctttatat tcattgtatc caattaaata tcctttttca tatgaacttt
     2341 ttaaaatctc tttttcatat aaattatcag atttggcttc tgtgacactt aaattataaa
     2401 gccctttaca cccatcttca taacctttat tatacccttt ttctgcaaaa tagtttttat
     2461 gatttttttc gttatgtact gaagatattg ttactccaga attttctttt gtactagcat
     2521 aaggacaaaa acctttattg tgttgatgtg ctgaatgtcc tccacaataa taacgtgtta
     2581 actctgattt atttttatta tgatgacctc ctgatgaatt atgccctcct gagtgagcat
     2641 atgaaaaatt ccctacacta atcatagcta aactaattac tacagctact aaccttttct
     2701 taaccataat ctattccccc caaaatgtta tatattttta ttatatcatc tactatatta
     2761 tatttaaatt cttaatatac aaaatattac tctattctgc taataaacat gtttaaacaa
     2821 cataaaaaac ctcctaatat ttgattaaaa atttacacac tcatgcatag catgtctcaa
     2881 atattaaaag gttttatact aataattatt tatcttttcc atacatttta gctaattggt
     2941 cttgtatctc tttactttct aaatactcat caaagtccat ttttctatcc attattccat
     3001 ttggagttat ctctataatt ctatttgcta tagtagatat aaattcatgg tcatgagaag
     3061 taaataaaag cactcctggg aacttttcaa gtcctttatt tacagaagtt atactttcta
     3121 ggtctaagtg gttagtaggg tcatctaata caagtacatt tgcatttgaa agcattagct
     3181 tactaagcat acatctaacc ttttctcctc cagatataac ttgagcttct tttaatgctt
     3241 cttcacctga gaataacatt ctacctaaaa atcctcttat aaaactttca cttttttctt
     3301 cagagaactg tctaagccag tcaaccaaag aatattctac tccgtcaaaa aacttattat
     3361 gattttttgg aagataatct tgagaagtag ttactcccca tttatactct ccactgtctg
     3421 gttcaagttc tcccataact atgttgaaaa gtgcagtagt agctatttca tctcccataa
     3481 atactacttt atcatctcta tccaatctaa atgacacatt atcaagaacc ttaaccccat
     3541 ctatagtttt agttaaattg tgtacttcta aaacttcatt cccaatttct cttgcaggtg
     3601 taaatcctac atatggatat cttcttcttg atggttgtat atcttcaact tctaatttct
     3661 ctaactgttt ctttcttgat gttgcttgtt tagcctttga agcattagaa ctaaatctag
     3721 ctataaattc ctttaattga gctatctttt cttcagtctt tttattttgg tccttagcca
     3781 attgtaaagc caattgactt gactcatacc agaaatcata gtttccaacg tacatttgta
     3841 ttttaccaaa atctacgtca actatattag tacatatttg atttaggaaa tgtctatcat
     3901 gtgatactat tataactata gattcttcta aatccattat aaaattattt aaccagttta
     3961 ttgatttaaa atctaagtgg tttgtaggtt cgtccattaa tagtatttca ggcttaccaa
     4021 aaagtgattg tgctaacaat accttaacct tttccccacc aactagttcc ttcatttgtt
     4081 tgtaatgcat atctttcgtt atgccaaggc ccataagtat tttctcagca ttagtttctg
     4141 catcccatcc gtcaagctct gcaaactcac cttcaagttc ggcagctttt atcccatctt
     4201 cttcattaaa gtcttccttc atatacaatg catctttttc tttcattatg ttccaaagtc
     4261 tctcatgacc tcttataaca acatttaaaa cagtttcttc ttcatattca aagtggtcct
     4321 gttttaaaac tgacattctt tctttatcag ttatagatat actacctgta ttaggctcta
     4381 tttctcctga taatattttt aaaaaagtag attttcccgc accattagct cctattattc
     4441 catagcaatt acctttagtg aattttaagt taacatcttt aaatagttcc ttatcaccaa
     4501 atctaagtcc aacacctgta acttgtaaca ttaatatatt ttcctttctt tttgctcata
     4561 atttatttta tcataaaact ttgataaca
//
LOCUS       DAKBNN010000257         4583 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000257 DAKBNN010000000
VERSION     DAKBNN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.263"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            12..3053
                     /locus_tag="OUB02_003562"
     CDS             12..3053
                     /locus_tag="OUB02_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HCT8887285.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNVFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(3214..4488)
                     /locus_tag="OUB02_003563"
     CDS             complement(3214..4488)
                     /locus_tag="OUB02_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCT8887286.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSINDK"
BASE COUNT         1676 a          664 c          693 g         1550 t
ORIGIN      
        1 ggtaatgtaa tatgtttaag caaaggctct ctaaattatt atctagtaca ttagtattga
       61 gtatgttatt tactgctgct ccaaatatta cttttgctga taatacaaaa gataattccg
      121 aaaaatatca aagttctgat atagaattac atgattatag taaaaacgcg gaatcttaca
      181 caaaaactaa agctcttgct aaagagaaaa tccaaacttt gctatcaaag tatggtgctg
      241 taagtgctca atatgctttg attgataatg gcaaaattga gatttctggt aatggtggag
      301 tttacagcaa acaggataac aaaaacttaa ataaggataa tatgtatagt atcgcatcta
      361 tcagtaaaat gtttactaca acagctgtta tgaagttggt tgatgatgga aaattaaatt
      421 tagatactcc tgttgtaaag tatatacctg aatttaaaat ggctgatgat agatacaagg
      481 aaatcactcc aagaatgtta ttaaatcatt catcagggtt gatgggaagt agttttaaaa
      541 acactatttt attagcagac aatgattctt atggtcatga taatttctta aaagaattac
      601 aaaaacaaag attaaaagct aaaccaggag ctttttccgt ttattgtaat gatggattta
      661 ctttagctga aattttagta gaaagagtat ctggtatgtc atttactaat tttcttgata
      721 aatacataaa taatccactt aacttacaaa atacaaaaac tacagaaaat agctttgata
      781 gttcaaaact agcaaaagca tacgtacctt attgggaaga tgcagttcca caagataacc
      841 ttaatgctat aggtgcaggt ggtttatatt caagtgcaga aaatctatgt acatttgcac
      901 aaacatttat gaaaaattca aatggtattc tttctcctgc ttcagtaaaa gctatggaaa
      961 acaaagaata tttaaatggt ctatggcctg aaggagaaga ctctatactt ggatatggtt
     1021 tagggtggga ttgtgtcaac acttatccat ttaatcagta taatttaaaa gctcttacaa
     1081 aaggtggaga ttctttatta ttccacagta atcttatagt tcttccagat gaaaatatgg
     1141 cagttgcagt tctttcttct ggtggaagta gtcaacttaa tgaaataatt ggacaagaaa
     1201 tcttactatc tgcattaaaa gaaaaaggta aaattaaaga aataaaacct gataaaacat
     1261 ttagtaagcc acaacaagtt aaaatgccaa gctctttaaa agaaaatagt ggtctttatg
     1321 cttcttcaaa tatgataaaa gtagatgtta atgataatgg tactttaaca gtatcttctc
     1381 cttatataga aaatggacct gaagataaat acgtatacat aggtcaagat agatttgtat
     1441 ctgaaaaagg taatagttgt ttaaaatttg taaaagaaaa aaataatatc acatatttaa
     1501 atatgagctc ttatgatgat gtccctggat taggtcagac agcaagttta tactatgtag
     1561 ctcaaaaagt agatgacaac aatatatcaa atagtgtcaa agaagtgtgg aaaaaaagaa
     1621 gtggcaaagg ttactatcta gtggatgaaa aatatacttc tcaaagctat atgtttggct
     1681 ctgtaaaagc aagttttagt ctttctgatg aaactccagg ttatatagtt aacactaaaa
     1741 taatggatga gaataactca aatgctttta tagaaatacc tggtgtaata ggtcgtgatt
     1801 tatccgacat taaacttcat aaagaaaatg gtacagagta tttatctttt ggaacactaa
     1861 cgtatgttag tgaagattct ataactaatt tgccagcaga aaaatctttt acttgtgaac
     1921 tagaatccaa tggttatgca aaatggtata agattggtga cgatatcgca aataaaaaaa
     1981 tagaagttaa tcttcctcaa aattcagctt ttgctgttta tgatgataaa ggtgttcctg
     2041 taaactattc tttagttaca aagaacaata gagttagatt gccaaaagga ggcgtaattg
     2101 ttttcttagg aagtccaaat gctagatttg aggttacata tcaagatgaa gtaaatgcaa
     2161 gtgcacttac aggaactgat agatatgaaa cttctattaa gataagtcaa gcaggttggg
     2221 aaaatgctga aaatgcagtt ttaataaacg attcagcaat tgcagatgct ttggctgcaa
     2281 caccatttgc atacaagaaa aacgctccta tattattgac tggaagctct caaataaatg
     2341 aaaaaacttt ggctgaatta aagagattaa aagttaaaaa tgtatatgta gttggtggag
     2401 aagcttctat aaatgaaaaa tctttagata ctataaaatc aaacaatatc tctgtatcta
     2461 gaatttcagg aagtgataga tatcaaactt ctatgaacat tgctaaagaa ttgaacaata
     2521 ttagcaatat atcaaagatt tcagtagtta atggcgaaaa aggattagcc gatgcagtaa
     2581 gcattggagc tgtatcagct caaaatgata tgcctataat acttactaat gaaaatagta
     2641 atataactga aataaataac gtatttaaaa acaaaaagat tgataaatct tatgtaatcg
     2701 gtggagaata tacagtttct aaaaatatag aaagtaaact tcaaaatcca cagagaatat
     2761 caggtagcac tagaaatgaa actaatgcaa aagtaataaa agaattttac aaggattcta
     2821 agatagacaa tttatatgtt gctaaaaatg gaatgaataa acaagatgat ttaatagacg
     2881 gattatctgt tggagttttg gctggaaaaa ctaaatctcc tgtaatgcta gtaggaaact
     2941 cactagatta taatcaaaaa gaactattta aaactatgag atttaaatct gtaacacaaa
     3001 ttggtggaaa tggaaatgaa aactcattta agcaaataaa agaaatagct taacataact
     3061 aaaaaaggct gtattcaagt aatattaatt gaatatagcc tttttttatt taagttataa
     3121 gttttatatg tgtaaaaatt aaatttaaat attaaatgat aagtttatta agatatagat
     3181 aagtctaaaa ctttgccttt tttaagttag tttctattta tcattaatac ttaacaattt
     3241 atctaaatat actgacttta attgtttact ggctaaaatc tttttagctg gttcaagctt
     3301 taatatagct gacagtatag ctcccatagc tctatgacta gccagagcat tattatcaaa
     3361 aattaaattt tgtaactgcc ctttttcaat tgccataaga actgctctat gtactgaatt
     3421 tgctggagtt attatttttt catctctctt taataacata atgctatttt tatggcaatt
     3481 tctaacacaa actccacaac ctagacatct atcctcatca atttttatat attctttacc
     3541 atcttcctta accttgctta tcgcatctat tggacatgca gttatacatt taccacactt
     3601 tacacaattt tcatggttta tatttggtat aaaacttgtt gtctgtactg gatgaagatt
     3661 tccaaatctt ttagctgcta ccattgcttc acagcaacat ccacagcaat tacatataaa
     3721 cgtaacccct ttcctaacat tttctccaca ttgaactaag ttatgttcat atgcttgatg
     3781 aagtagttct ttacattcta ttttatccac acgtcttgca aatttattat ttattaaaga
     3841 atttgctaca ttgtcaaacg tcatacatat atccatggga gcatcacaag cttttccaac
     3901 atgttgcatt ctatgtctac agtaacacat acttatacct atatgagttg attcatcaat
     3961 tatatgagta gccctttcat aatctaaaat actaacctca ttatcattag ttaaaacttc
     4021 ctcttgaacg tatactcttc caagcttcgt ttctgttgaa taaaataaat ctttgataaa
     4081 atcttcttct acattcatat attgatagta aagctctgcc aaaagttttt ggtctatatc
     4141 gtgtctcgtt ctcatcatgg caaactcaaa aaaaccagcc attggaggag gcattatata
     4201 tttcttccct ttattatctt ctatatctag tatcagagcc ttactagcaa gtttatccaa
     4261 tactctatat gcttcacttt cacttacact ccaaatttta gctgctgttt ttactctgaa
     4321 aggctttatt ggaagctgag caactaactt cgcctcttgt tctgtatata gtacatttag
     4381 tattttgtat aatgtatcag atggtggagc accttgtgga aacttgttta ttctttcctc
     4441 taaactttta tacatattct tcgcagttat atgagaaata gtaatcacct tctttttctt
     4501 ttgttttcca ataaaatcaa atattctttc ttattatata ttctcttact ttatataata
     4561 ccaattcttt ctaaatagtt aaa
//
LOCUS       DAKBNN010000258         4574 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000258 DAKBNN010000000
VERSION     DAKBNN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.264"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            335..4477
                     /locus_tag="OUB02_003564"
                     /pseudo
     CDS             335..4477
                     /locus_tag="OUB02_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
BASE COUNT         1748 a          684 c          795 g         1347 t
ORIGIN      
        1 aaagaataaa aaataacaaa aaaatcactg acatttcact gacaaattgt aattttgtta
       61 ttataaatac attgaaaata cttgattatt gaaaaatggc ataaaataat acattttatg
      121 ccatttacta ttatttgata gtttttaact tatgacataa gatatttttt aacatataca
      181 ataaaatata aaaaattgat attacaaata aaaaaaatac atattagatg ttatagaaaa
      241 aaagcgcact tataggctta aaatgtcaca taaaagagtt ttaaagaata atatattatg
      301 aacacaaatt ttgtaatcgc aaaggagtga taatatggca acagtatcag gacaaatagt
      361 ttttgataga aatagaagtg ctaccattga ttctggagac tcaggaatag ccaatatacc
      421 agttgtatta caagatatag caactggagt tagacttgtg gttctcacag atgcatcagg
      481 aaattatgtt tttactaatg ttccagatgg taactataga attgtagaag cttttggaac
      541 tacaggagga gtaccaacac caggggattt tgcaacagcg atagtaggtc cagttccaat
      601 tgcagaaatg ccacctatta gttctgtgac aaatcctcct attggttcaa caaacttaga
      661 tggtacaaca cctaacacac tattaataac agtagcaggt tcagatataa caaatcaaaa
      721 tatattaaat gggcctgtga tttatacacc tatagagaat atattggatt cttgcgtatc
      781 tgtatctaat ataaacctta ttacagatgc agataacgga acattcggtt tttttccacc
      841 aggtacacca agtaatacag gacctgaaat agaaccttac ccaaatgtga cgccagattt
      901 tacgtatgtt gtaccagacc caactaagtt tacaccttta gatggagaat atacagtaca
      961 aaatattatg acaaatgcaa tgagtaatgt tataggagct tggtggcgta ttgctgacca
     1021 tactacagga aatgaaacag gaagaatgat ggtggtaaat ggttttaatc ctggagctgt
     1081 attttttaaa tcaacagtac aagttacacc taacactaat tatttattta gcacttggat
     1141 tttaaacctt tttaaggtaa caggatatcc accaccacag ctaggtgtaa gaatatttga
     1201 ccaaaataat aatatactat atcaagcaac attaggagct caaattccag ttaatacaaa
     1261 cgcaccagaa tggaaagaag taggtactgt gattaattct caaaataata atcaacttac
     1321 agtagaattt tttagtgaag gagaaagcgc cattggaaat gactatgcta tagatgatgt
     1381 ttctttaaga gaaatagctg ttccaacttt tactccaata aaaacaagta acacagaaac
     1441 tgctaatgta ggtgatataa tcacatatac agttacatta gataatactt gtgatagccc
     1501 tttaactaat gtatttttta gggatttaat tccaaatggt ttagagttta tacctggaac
     1561 tgtaacggtt gatggagtat ctgtaccagg agtagaccct aatacaggtt tcccacttcc
     1621 agatattgga ggaggaacag gggttgaagt aacttttgat gttgtggttg aaagtattcc
     1681 taacccaaat cctactaata atatagctaa tatagattac tcctatacac ctgtagaagg
     1741 cggtatacca aatgattttt cagtagattc taatccagtt cctgttgaag taatatcagc
     1801 agatattgaa gttacaaaat tatctgaacc tactatagtg aatccaggag aggaattaat
     1861 atacactata aaagtagtaa ataatggacc attcccatct gaaaatgtgg ttttaacaga
     1921 tgatgtacca gcttctattg taaatccaga gtattcatta gatggaggag taacttttca
     1981 accatggact ggaagtttaa atatagggac attagaagta ggagagacaa gagttattat
     2041 aattaggggt attgtaaatc catcaacagt aggaattata acaaatacag ctgtagtatc
     2101 ttcaactaca gcagacccaa accttaataa taatgcatct acaatagaga cagaagtgaa
     2161 tttattagca gatattttag taatgaaaac agcagaacct aattcagctg ttccaggtac
     2221 tttattgaga tatactattc aagtagaaaa cttaggacca gcaaatgctg aaaatgtaat
     2281 attaaatgat gatattccag cttctattat aaatccagaa tattcattgg atggaggagc
     2341 aagttttcaa ccatggaatg gaagtcttaa tataggtact ttaaactctg gaatttcatt
     2401 aacagtttta attcaaggaa cagttagttt aaattcttca gaatatatag taaatacagc
     2461 aacagtttct tcaactacac ctgacccaga tttaagcaac aatatatcta ccattattac
     2521 accagttaac ccacaagcag gtatttctat tataaaagta gctgacgagg atgtagctgt
     2581 tccaggagaa gaatttgtat atacaattga aatttttaat gaaggaccat ctaatgctac
     2641 gaatgtagtt ttaacagatg atataccaga tgttatttta aatccagaat attcattgga
     2701 tggaggagca actttccaac catggactgg aagtcttaat ataggtacag ttgcaccagg
     2761 gcaattaata agaattatca taaagggatt agtaagttca actgctacag gagatattac
     2821 taatacagca gaagtaagtg cagatgtacc agaaccagtt acggattcat caacagtgac
     2881 aactcctata gttccatcag cagatataga agtaataaaa actagcaata tggatacaac
     2941 agttccagga gaaactttca gttatactat aacagttaca aatttaggac catctgctgc
     3001 acaaagtatc gttttgatag atgatatacc agatgttatt ttaaatccag agtattcatt
     3061 ggatggagga gtaactttcc aaccatggaa tggaaatctt agtataggca ctttggatgc
     3121 tggagaaata agaagtatta taataagagg aacagtgagc cagacagcag taggtacaat
     3181 tataaataca gcaacaataa gttcaactac acctgaccca aatccagata ataatacttc
     3241 aacagatgaa acagatataa gtccattagc agatatttct gtgataaaag gcaacgaacc
     3301 agttgctata ccagggggaa gatttattta tggaattgaa attgcaaatg caggaccatc
     3361 ttttgcagag aacgtaactt taacagataa tataccagct tctattttaa atccagagta
     3421 ctcaatagat aatggagtga ctttccaacc atggaatgga agccttaata taggtacttt
     3481 agatgctgga gagataagaa atatcataat aaggggaaca gtaagtcaaa cagcaatagg
     3541 cacaattata aatacagcaa cagtaagttc aactacacct gacccaaatc taaataacaa
     3601 tacttcaaca tcagaagcag aggtaagttc atcagcagat atttctgtag taaaacgaag
     3661 caatcagact gttgttgtac caggagatgt actagactat acaattgagg tgagaaatgc
     3721 aggaccgtct actgctcaga acgtaacttt aacagataat ataccagctt ctattttaag
     3781 tccagaatat tcaatagata atggagtgac ttttcaacca tggactggaa gtcttagtat
     3841 aggcacttta gatgctggag agataagaaa tattataata aggggaatag taagtcaaac
     3901 agcaataggt acaattataa atacagcaac agtaagttca actacacctg acccaaatcc
     3961 agataataat acttcaacat cagaaataga tataagttca tcagcagata tttctgtaat
     4021 aaaattagca aataaaacag aagcatgtgt aggagagcaa atagattttg taatagtagt
     4081 atcaaatgca ggaccagaaa atgctcaaaa tgttacttta atagacaatg tttcagataa
     4141 gcttaagaaa gcagtatttt cacttgatag aggtgtaagt tttcaaccat ggactggaag
     4201 tcttaatata ggtacactac cagctggaac tttaagagtg attctattaa gaggaattat
     4261 aaaatcaaca tgtcttgata gattaacaaa tattgcataa gttacttcaa cagcaccaga
     4321 ttcaaacctt aacaataata tatcaagagt gcaagtagaa ataaaacaat gttgttgtaa
     4381 tgattgctgt tgtaacaact gtagtaattg ctgttgcaat gattgtggtt gtaaagattg
     4441 ttactgtaat tgttgtaaaa atgacttttg gtgttgattt tataaaataa ttttgtatac
     4501 cttaaattaa actgaaataa aaaataaatt cacatatgag taaaatattt tataaatatt
     4561 aataaatttt ctat
//
LOCUS       DAKBNN010000259         4563 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000259 DAKBNN010000000
VERSION     DAKBNN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.265"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(442..2829)
                     /gene="cadA"
                     /locus_tag="OUB02_003565"
     CDS             complement(442..2829)
                     /gene="cadA"
                     /locus_tag="OUB02_003565"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCT8887287.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKISIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEADDVINKIKEIINSTEPGLD
                     IQVESTNKVKGRTTEKPGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGISMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2852..3217)
                     /locus_tag="OUB02_003566"
     CDS             complement(2852..3217)
                     /locus_tag="OUB02_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887288.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3417..4250
                     /locus_tag="OUB02_003567"
     CDS             3417..4250
                     /locus_tag="OUB02_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887289.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNMNSNMGIPN
                     NMSNNMNSNMGMPNNMSGNMNNMYGNTSMPNNMNSNMGYNMNNTMSIPNFSCNQNMPP
                     NVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQTTNIEEFDEEE
                     M"
     gene            complement(4398..>4563)
                     /locus_tag="OUB02_003568"
     CDS             complement(4398..>4563)
                     /locus_tag="OUB02_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887290.1"
                     /translation="FIGTYNENDCNYINEISQSNYSDYIKFEEKFFFEQNALYDFAES
                     GSLDFDDFLE"
BASE COUNT         1557 a          792 c          477 g         1737 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagttatac tgttgtttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa atcttctata tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatgtgtaat
      301 gaatatagat ttatatatac aaaaacgctc caataaaaag gtaccaataa agttaaaaga
      361 aacttattga taccttttat tattatatac ttcaatatat aattataagt tttttgaata
      421 gtatggattt acatttatcc cttatctcat agctctcata gcatttacta ctgctataag
      481 tgccacacct acatcagcaa atatagcttc ccacatagta gctactcctc cagcacctag
      541 tatcataact ataattttta ctcctagagc aaatattatg ttttgccaca ctatcttatt
      601 agtcttgttt gctatttcaa ttgctttaga tatcttgcta ggctcatctg tcataagcac
      661 tacatctgca gcttcaatag cagcatctga acctaagcct cccatagcta taccaacatc
      721 agcacgagct aatactggag catcatttat tccatcacct acaaatgcta ttttctcttt
      781 ttcagttctt ccttcatata aatcttctaa tctatctacc ttttcattag gaagtaaatt
      841 tgagtaaact gtatccaatt ctaattcttg agcaatattt tttgctactt tctcattatc
      901 tccagttaac attacaactt cttttacccc tattgctttt aagcttctta tggcattttt
      961 actatcttct tttacttcat cagatataac tatataacct ctatatactt tatcaacagc
     1021 aatatagact actgtaccta cttctttagc tgatgaataa gatatatttt cttttttcat
     1081 aagtttctca ttacctgcta atataaaatt gccattatgc tttactctaa ttccataagc
     1141 tgctatttcc tcataactgt ctattgtatc taaatcaatt tttttattat agtaacttaa
     1201 tatagacttt gctattggat gatttgaatt tacctccgca atagctgcat attctaataa
     1261 ttcttcttct gatatacctt caggatttaa cttagttaca ttaaacacac ctttagtaag
     1321 tgttcctgtt ttatcaaata ctactgtatc tacacttctt aaagcttcta aatagttact
     1381 tcctttaata aggataccat ttttagatgc aaacccaatt ccactaaaga aactaagtgg
     1441 tattgataaa actaatgcac aagggcaaga aacgactaag aatatcaatc ctctatataa
     1501 ccaatcacta aatacctcac ctgagattac aagtggtggc acaaatgcta ttaataaagc
     1561 agctattact actatcggag tatagtatct tgaaaactta gatataaagt tttctgtctt
     1621 tgacttttta atgctagaat tttcaacaag gtcaagaatc ttagaaactg ttgattctcc
     1681 aaaactcttt gtaacttcaa tactcaataa agcattttta tttataacac ctgataaaat
     1741 ttcgtctcct ttttcaactt ctctcagtac tgactctcct gtaagtgcag aagtatctag
     1801 catagaaatt ccatccacta ctacaccatc taatggtact ttctcaccag gttttactac
     1861 tattatatcg cctacattta cttcttctgg tgacaccacc ttaacttcag aattaacttt
     1921 taaatttgca tagtctggtc ttatttgcat aagtgaagtt atagactttc ttgatttccc
     1981 aactgcaaca ccttgtaaat actctcctac tttatagaaa agcataaccc ctactgcttc
     2041 tgataactcc cctattgcta atgctcctac tgttgctaga gccatcaaaa agttctcatc
     2101 aaatactctt ccttttgata tatttcttat tgctcttaaa agcacatcgc cacctactat
     2161 tacataggca actataaata ctatattgct aaactgactt tcataacctg tagctgtttg
     2221 atatatacca aatatatata caagtgctcc aattataagt ggaattaatt ctttcttatt
     2281 tgtatcattt acagctcctg gtttttcagt agttctccct tttactttgt tagtagactc
     2341 tacttgtata tcaagccctg gctctgttga atttattatt tcttttattt tatttataac
     2401 atcatccgct tcaaagttat tacttatatt tactgtaagt tttttattta caaagtttaa
     2461 attagaagat tcaacttcct ttaatttaga aactttgtta ttaatttcct ctgcacaatg
     2521 tgcacagttt aatcctccta atataagctc ttttttaatt gatattttac tagatgcatt
     2581 ttctttagaa cttatttgta tatcaagacc tggttctgtt gaatctatta tatctattat
     2641 cttttctata gtagtatcct cattaaagct ttcctttata tttactgtaa gttttttatt
     2701 tataaaattt aaattagaag atttaacttc ttgtagttta gaaactttat tattaatttc
     2761 ttctgcacag tgagcacaat ttaatcctcc taggataaat tcttttttaa ttaaattatt
     2821 tgcttccata tatattcact cctctcattt atcatttata agtctctcta atatgattta
     2881 atccacaatc aaatatctga cttatatgac tatcatctaa tgagtaatac actgtttttc
     2941 cttctcttct aaactttact aatctagcct gctttaatac tctaagttga tgagatattg
     3001 cagagtgagt catatttaat aaactcgcta tatcacatac acacatttca tttgcaaata
     3061 atgcatataa tatctttact ctagtagtat ccccaaatac tttaaataat tctgctaaat
     3121 catataacat ctcttcatct ggcatagttg atttagcctc tgttactatt tcttcatgta
     3181 ctatattaca attacaatca tttatctctt ctctcatttt taatcctcca attattttaa
     3241 tatatgaata actgttcata tgttcattat atgctatcat ttctaaattt gcaacaataa
     3301 tttttattaa tttaatataa ttagaatgca catattataa acttttttaa tatatatatt
     3361 attagaataa gtttttgtaa acttttttat agttttctag gaggtgcaag tttaatatga
     3421 gtaacaagaa aaaaaaggat ttagatacta gttacatgcc ttacttagac ccagcgaact
     3481 atgcagatta tattactaat gaagaaattc ctcaagatga attgagaaat gaccctcaaa
     3541 tttctaatgg ttatacaaaa atacctaata accagaacat aaataaccta tatcctaata
     3601 atgtaaatca ttatcctaaa agtagtaatt ctccaaacaa cttgcctaac acaaataata
     3661 catccggaaa tatgaattac aatacgtctg ctacaaataa tatgcctaat aacatgaata
     3721 ataatacggg tatgcctaat aatatgtcta acaatatgaa tagtaatatg ggtatgccta
     3781 acaatatgcc taataacatg aatagtaaca tgggtatgcc taacaatatg cctaataata
     3841 tgaatagtaa catgggtatg cctaataaca tgaatagtaa tatgggtata cctaacaata
     3901 tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggt aatatgaata
     3961 atatgtatgg taatacaagt atgcctaata acatgaacag taatatgggg tataatatga
     4021 acaatactat gagtattcct aacttctcat gtaatcaaaa tatgcctcct aatgtactta
     4081 tgatgatacc aggagttata tgtcataata caatgcaagg tatgcctgta gttatgccta
     4141 gtactatgcc tcctaatata tatcctactc cttatggttc tagtaatatg tctatacaag
     4201 gtataccaca gactactaat attgaagaat ttgatgagga agaaatgtaa tatatatttt
     4261 tttaagtaag ttgtataaaa cattaataat tgaccataat aataaatgta ttcctcataa
     4321 tattccccca atattataga aataaattta agcgagatta aattcagtta atctcgctta
     4381 aataatgtct aaatttatta ttccaaaaaa tcatcaaaat ctaatgaacc tgattctgca
     4441 aagtcatata atgcattttg ttcaaagaaa aatttctctt caaatttaat atagtcacta
     4501 taattagatt gacttatttc attgatataa ttgcaatcat tttcgttata agtaccaata
     4561 aaa
//
LOCUS       DAKBNN010000260         4519 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000260 DAKBNN010000000
VERSION     DAKBNN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.266"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            142..918
                     /locus_tag="OUB02_003569"
     CDS             142..918
                     /locus_tag="OUB02_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HCT8887291.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(1010..1762)
                     /gene="xth"
                     /locus_tag="OUB02_003570"
     CDS             complement(1010..1762)
                     /gene="xth"
                     /locus_tag="OUB02_003570"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCT8887292.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1923..2873)
                     /gene="galT"
                     /locus_tag="OUB02_003571"
     CDS             complement(1923..2873)
                     /gene="galT"
                     /locus_tag="OUB02_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCT8887293.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3258..4454
                     /locus_tag="OUB02_003572"
     CDS             3258..4454
                     /locus_tag="OUB02_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCT8887294.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         1646 a          680 c          557 g         1636 t
ORIGIN      
        1 aatattatac atacaatagt ttaacatttt tacaaatatg gggtatacta aaataaaagt
       61 atttttattt gaaaatttaa ttttatttat aatataatct atagtaaaag aaataataaa
      121 aaaatttagg agataaactt tatggaatac ataattttcg atttggaatt taaccaagga
      181 tttgataaaa aactcaataa aactgtatcc aatgaaaagt gtccttttga aattatacaa
      241 ataggagcaa ttaaattgga ttctaaattc aatataatag atacgtttaa tagctatgta
      301 aaacctacaa tttataaaga catacatccc tttattagtc gtatgactaa tattaaaaat
      361 tctgatttta atgattcgcc tactttccct gaggtctaca acaattttat aaaatttata
      421 tcatcacaag accctatatt atgtgtttgg ggagctggag atttaaagga attatacagg
      481 aatatcaact atcacaagct accatcaaat tccttaccta aatcttatat taatatacaa
      541 caacatgcat ctaaatattt caataatcca gcaggaaaaa gcattggcct acaaaatgct
      601 atttctatat tagaacttga tgaaaaaatg tcttatcaca atgctttaaa tgatgcatat
      661 tatactgcta aagtttttat taagatatac aatccaagta tagtcccaga tatatatctt
      721 tatacgagta taaaacctaa aactataaga tattctaata aaaaaagagt tgattatgat
      781 aagttatttg atgaatttag gaaaatttta aatcgtgaat taactaaaga cgagaaaaaa
      841 atcattaact tagcttataa tatggggaaa acaaatcaat ttacattaga aaatgttaag
      901 caaagaaaaa acaaatagag tttatttaga taaaaaatag gcatgtagat taaaaaacta
      961 gttaacttta aatttatatc tacagcccaa tttttatcat aattaatttt tattttatct
     1021 ctaattcaac tggacaatgg tctgaaccta atatttcagt gtgtatatcc gcacttacta
     1081 atctatcttc taattttttg gatgcacaaa aataatctat tctccaccct gcattatttt
     1141 ttcttgcatt aaatctatat gaccaccaac tataaacacc ttctttatca ggattaaaat
     1201 atctatatgt gtctataaac ccactgtcta ataatttaga aaacttctct ctttcttctt
     1261 tagtaaaacc tgcatttttc atattatttt taggattttt taaatctatt tctttatgtg
     1321 caacgttcat atctccacac acaattacag gcttatgatt atctagtttg gttaaataat
     1381 ctataaagtc gtcttcccat ctagttctat attctagtct ctttaattca gattgagaat
     1441 ttggcgtata tactgtaaca aagtaaaaat cttcaaactc tagagttatc actctaccct
     1501 ctttatcatg ttcttctata tttattccat acattacttt taaaggctct ttttttgtaa
     1561 atatagcagt acctgaataa ccctttctct cagcataatt ccaatattga aaatatcctg
     1621 gtaaatctaa ctctatttgc ccttcttgca atttagtttc ttgtaaacaa aatatatctg
     1681 catctacttc tttgaaaaag tctaaaaatc ctttcccaac acaagctctt attccattaa
     1741 cattccatga tataaatttc atacttatac tcctcatcta aacattgaca cactccccat
     1801 agctaaagat gggggctttt caacaatttt attgtaaatt cttaattcac ttttatttta
     1861 acaatgctat actataattt catctaaaat tactatttac ttaataccta aaaatatatg
     1921 ttttaatcaa tttttaactt ttttgttaaa tcttcagggc tcattgaaga aacatacata
     1981 tttgttccaa gttcaaatcc tccaaaatta tattttcttg ggattatgtg catatgaaca
     2041 ttaaaatacg attttgtttc aatctttgtt ggatgagtgt gaatacataa attaaatggc
     2101 atatatccat tgatattgta taacttctta aacaactttt caaatattgt agaaagctcc
     2161 tgtatattat tatcatttaa ctcaccaaac tctattttat ctttaaatat tattcttact
     2221 tctccactat acttggttgc atatggtata agtaccaaaa aagcttctcc attataaaca
     2281 actcttctat ctaaacttat ttcattttct attaaatcat catacaaaga gcttttattg
     2341 ctgtcataat actcctttga aaccaacagt tcattcgtaa tttcaggtgg aataaaagac
     2401 atagacaata tttgtgcatg tggatgcatc aaagatgctc ctgcctttct caagaagttt
     2461 ttgaagatac taacatactc tactttatca tctttcgaca aatctctaaa tctatttcta
     2521 tacataaaaa aaacatcttc aaattctttt tgactcatgt tataaaaact acgattatgt
     2581 ttatcgctct ctatcattac ttcatgaaca ccatatacat ctcttgccat atcatcaatt
     2641 ataggaaatt tattgtagac agattttaca agccatcctt cttcactatc tatttcaaat
     2701 aatgcatcag ttgcatgagc ttcatttcct ttgcaaaatg ggcattcttc atcatattca
     2761 ttagtttctt catcttcatt tgggatatct gctgtatcta aaggtctttt taacctatct
     2821 gtagcaaata taacaacatc attggtaatt ggatctattc ttaattcttt cataattaac
     2881 acctcttagt taatagcctt taatataatt atatgatatt tatcgtatct gttaaataaa
     2941 tttataatat tttttataat tttgcatatt tattattata tattttgtct atagctgaaa
     3001 gattataact aaaaaatcac tatcagtata tcagaatatt atcttactat aaattaattt
     3061 atgaatacat aatttagtgg cataaacatg ggtattttac tataatttgt taaaagttta
     3121 ttttaaagct atattaattt tttttgatga tttttaccca taagtatgtt attattatct
     3181 tgtaaagatg tcttgtattc aaacatacaa tttacataat acaatatacg tatttaaatt
     3241 aaataggggg tattaaaatg aattattcaa acagagttag tgcaatgcaa gcctctccaa
     3301 tcagaaaact tgttccattt gcacaagcag caaaagataa aggaatcaaa gtatatcact
     3361 taaacatagg acaaccagac ataaaaactc ctaaaggatt ttttgatgct gttaaaaact
     3421 ttgatagcga agtcttagaa tatgctacat cagaaggtat tccagaatta ttagaagcac
     3481 ttcaaaatta ctataaaact tacgatatga attttgaaaa agatgaatta ttagtaacta
     3541 atggtggtag tgaagcttta cttttcacta tgatggcagt ttgtgaccca ggagataatc
     3601 tattagttcc agaaccattc tatacaaact acaatggatt tggtcaatct gtaaacgtag
     3661 aagttaatgc tgttacaaca aaggcagaaa acggtttcca ccttccatct aaagaagaaa
     3721 tattgtctaa agtagatgat aaaactaagg ctataattct ttctaaccca ggaaatccaa
     3781 ctggtgcaat ttatacaaaa gaagaactta atatcctagc tgaaatagct aaagaaaaag
     3841 atttatggat aatagcagat gaagtttata gagaatttgt ttatgatggg cttgagtata
     3901 caagctgtgg taacttagag ggtgtacagg atagagttat aataatagac agtgtttcta
     3961 agagatacag tgcttgtggt gctagaattg gttctatcgc ttgcaaaaat aaaggtctta
     4021 tcgctcaaat ccttaaatta tgtcaaggaa gactttgtgt tcctacatta gagcaaatag
     4081 gagctgttga gttatacaaa actcctgttt cttacttcaa agaagttaat gaagagtaca
     4141 aaaaaagaag agatgtatta tacaatgaat taatgaaggt tgaaggtgtt atatgtaaaa
     4201 aaccaactgg tgctttctat atagttgcta aattaccagt tgaaaatgca gaagatttca
     4261 caatttggat gttaaaagaa tttaacaaag ataatgaaac agttatggtt tgccctgctg
     4321 aaggattcta tgctactcca ggtttaggac gtgatgaaat aagattagct tacatcttaa
     4381 atgaaaaaga ccttcataga gctgctacat tattaaaaga aggtttagag caatatgtag
     4441 ctttacaaaa ataatttttt aaacttaatt tatataaatt taatgatata aaatgtcccc
     4501 ttttcagata ttatttgaa
//
LOCUS       DAKBNN010000261         4436 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000261 DAKBNN010000000
VERSION     DAKBNN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.267"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            60..1064
                     /locus_tag="OUB02_003573"
     CDS             60..1064
                     /locus_tag="OUB02_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8887295.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1190..2302
                     /locus_tag="OUB02_003574"
     CDS             1190..2302
                     /locus_tag="OUB02_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8887296.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAES
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2791..3552
                     /locus_tag="OUB02_003575"
     CDS             2791..3552
                     /locus_tag="OUB02_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887297.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3555..4373
                     /locus_tag="OUB02_003576"
     CDS             3555..4373
                     /locus_tag="OUB02_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HCT8887298.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
BASE COUNT         1858 a          416 c          794 g         1368 t
ORIGIN      
        1 taggatgtaa aaataaatag gatataaaaa tatcgtaaat gtatgaggag tgaaatttaa
       61 tgcgtttcaa aagaatattg atattgatgt taactgtagc aatgatagct ggaatgttgg
      121 ttgggtgtgc taataaccca agcaataatc ctagctctag caaagataat aaaagtgata
      181 taagcgataa aagtaattca aaaaaatctg tatcaatgat actagatatt gaaggaacaa
      241 ataatgaagc tatgaataat tcagctttat tagcattaaa caatgctcaa aagaaactta
      301 atatagatac aaacaaagtg gaatcagatg attcctcaac tttttctaat agtatagata
      361 ttttatgtaa tgacaattac gatttaatta tagctgtagg agctagattt gcaaaaccat
      421 tggagatggt agccaagaaa tatccaaagc aacaatttgc aattatagac tatgagtatg
      481 acaagcaacc ttctaatata acttcaattt cttatgaaga taataaatct ggatatctag
      541 ctggattaat tgctggaaaa atgactgaaa gcgataaggt tggttttata ggaggaataa
      601 agagctctag tagggataaa tttgaatcag gttttagaga gggtgttaaa ttttctaata
      661 gtagtataaa ggatatttca gttgagtatg cagatgtttt taaagatagc aaatcagtag
      721 aatctatagc taaaaaaatg atggataatg gagtggatat tattttttct actactgaag
      781 atgattcaaa agctgtaata gatgcagtaa gggcaaaaaa taaaaaagtt atagcaacga
      841 ataaagacca acatgagtta gctccagaaa atgttataag ttctattgta aaagattttg
      901 agaatcctac ttataatctg atacaaagtt ttgtaaaagg aaattataaa ggcggaaaag
      961 ttatagaaaa cacagttaag tctggaagta ctgggcttgc ccaaaatagt tctcaaaata
     1021 ttcctccaga tgtgttagaa tatgttaata agaacaataa ataatctact ataatatcca
     1081 agaatactta tgactttagt tatgaggtga attggatgtt ataatagaca tatatagaaa
     1141 gacatatgca gaggtaggtc agacctatct aaatcaggga gtgaattttg tgaaaataaa
     1201 aaggatattg atggcttgct ttgtagtagt ttttacaatt tttatattag ctatatgtaa
     1261 ttttgatttt ggatttgata aagtaaataa gaataattct aatatagata gtgctgaatc
     1321 gaaacagaat gagtctaaaa gtttgccaac ttcagcagaa aaggataaat caaatagcaa
     1381 aagtaagaat aaagaagatg acacagaaga tgattcatat gtaagaaaaa ctattggagt
     1441 attgttagat accgaaaact taaataacga atcatataat aacaatgtac tatcagcctt
     1501 aaaaagggct gacgaagatt ttgatgttag gataaaactt gaaaaaccaa agacttctaa
     1561 aagttttaaa gagagtatag aaaaactgca taagtatgaa ccagatttga taatagcagt
     1621 tggagccaga tttgcagagc caattaaaga agctgctact aaatatccca aacagcaatt
     1681 tgcaatagtt gactatactt ataaagttca gccagctaat gtaatttctg tatcatttca
     1741 agataatgtt actggatatt tagcaggact tattgcaggt aaaatgactg atactgggaa
     1801 agtcggtttt ataggaggag ttgagggttc tagtagagat aagtatgaat ctggatttag
     1861 aaaggggctt aaaacttcaa ataaagatgc tgagttacta gttaagtatt caaatatatt
     1921 tgaaaaccct gaatcagcta gaactattgc aaaggaaatg agagattctg atagagatgt
     1981 tgtattttca gctacagaag atgatagtaa aagagctatt caggtagcaa gagaaaatgg
     2041 tggtaaagtt atagctatta ataaagacca atacgatatg gctccagata atgtaataag
     2101 ttcaattata aaagatgtag aacaacctgt atatgaactg ataaagaacg ttgcaaaaga
     2161 tggttttaag ggaagtaaag taatggaatt taaaataaaa gatggagaac ttggcttgac
     2221 ttcaaagagc agtagaaata taccaccaga tgttttggaa tacataaaaa aagagtataa
     2281 caaagtgaaa gatacatatt aattattgag tagaagatta taaaaatata ctgattaagt
     2341 ttagattggt gataaaagga tatatttatt aaatttgtgc taactgtaca aaatatattg
     2401 attaagttta gattagtgat aaaaagatat atttattaaa tttgtgttaa cagtatggaa
     2461 tatactgatt aagtttagat tggtgataaa aggatatatt tattaaattt agtctaactg
     2521 tacaaaatat attgattgag tttagattag caataaaaag atatatttat taaatttgtg
     2581 ctaactgtac aaaatatatt gattaagttt agattagtga taaaaggata tatttattaa
     2641 atttgtgcta actgtacaaa actatactaa ttaagcttag attaactata taaaattata
     2701 acaataagtg tgtgggcaga aaaattctgc tacacacttt atttatattc acataaaaaa
     2761 tttatcaaaa aaaagaaaaa ggagtaaaaa atggaattta caagagaacc atttatacta
     2821 gatgacgatt tgtgtctagc tttggtggac aaaagaatta gttatctaat ggaaaatgaa
     2881 ttaaaattaa gaaatataga gataataaaa acaatagaat gtaaagaatt atatgaaagc
     2941 ataaaatatc acccggatat atgtatttgt aatttaggaa aaggagatat aattgttgcg
     3001 ccgaatgtat ataatcaata caaagagtta ttaagtagat ataaatttaa tataattaag
     3061 ggagaagcta atttattaaa tagataccct tatgatatac cttacaatat tgcgatagtt
     3121 ggtgactatg ctatacacaa ttttaagtat actgacaaag caatattaga ttatattgaa
     3181 aataataaac ttaaaaagat aaatatagag cagggctatt ctaaatgctc aatctgtata
     3241 gtagatagaa cgtctataat aacatcagat aaaggtattt ggaaaagtat gaagaataca
     3301 gaaatagagt gtttattgat agaaaaaggt cacataaatt tatttgagat gaattatgga
     3361 tttataggtg gatgtacagg gttgatttct aaggataaac tagctttttg tggagatgtt
     3421 aaaaaacatc ctgactatga acgtataaaa agttttgtag aaagtaaaaa caaggagata
     3481 gttacactta gctgtgaaaa tttactagat ataggttcta tagttcctct catgacaaga
     3541 aaggagcgat aactttggtt gagaaggata atatttcttc catgaaattt ttgaggaata
     3601 ataatgtaaa cgatagatta atatatacga atgacattgt agaagtagtg atagattctg
     3661 caaagcaaga agtgttaaat aaaaaaatac ttaatggtac taaagctgaa caaagagctt
     3721 taaatattga tgagatatca acggaaataa ctagtatgat aataagtata atgaaagaga
     3781 aacttttgaa agaatacatt ctaaaatgct atgggcgtac atatcctgaa gataaaaata
     3841 atatatacat atattcactg aacatatttg ctaaaaaaga gatttttatg agagaaacag
     3901 tatttactag ggtgagaaat tatatagaag aaaatgattt tataaatgta gaaggtttta
     3961 ttagatttag aatgagagaa tttatgaaat atatttctgc tataggtgat attgcagttg
     4021 aggagtattt aataaagaaa gaccaagatg aatttataag agttttaaaa tactttatag
     4081 atactcaaga agaaaagatt gatttgctta aagttcatat aatggaagat aatacatttg
     4141 ttttatacga taaaaatgga aataagatag acagtataga tgatgaagaa attataaata
     4201 tggtaataag agaaaattta aattatgagg actttttaat aagtacctta ttaactctat
     4261 gtcccaaaaa aatagaaata ttggatttgt taaataataa ttgctcaaag gaaattatag
     4321 atactgtaga agcaattttt gaaaataaag ttagtataat tatggaaaac tagaatctaa
     4381 gcatataaaa gctatccata taaaattaaa aaaattcttt ttaaatcgac acaaaa
//
LOCUS       DAKBNN010000262         4424 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000262 DAKBNN010000000
VERSION     DAKBNN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.268"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            224..1303
                     /locus_tag="OUB02_003577"
     CDS             224..1303
                     /locus_tag="OUB02_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCT8887299.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEDRMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1627..1995
                     /locus_tag="OUB02_003578"
     CDS             1627..1995
                     /locus_tag="OUB02_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HCT8887300.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2112..2909
                     /locus_tag="OUB02_003579"
     CDS             2112..2909
                     /locus_tag="OUB02_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8887301.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3308..4198
                     /locus_tag="OUB02_003580"
     CDS             3308..4198
                     /locus_tag="OUB02_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCT8887302.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
BASE COUNT         1685 a          488 c          832 g         1419 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atagtttaac agtaggaaat atcgtatgag
      121 taagactatc gtattttaca taaagattta gcaatatgac acactgttgt cgtgttctgt
      181 agggtatgat taataaatca gatatgaaag gagagtgagt aatatgccag aattactaaa
      241 aaatatgtat aatcgtgagt cactttatga agttgcagta gctatccaat cagtttataa
      301 ctcatttaag gtagacgagt tcataaaatc tacaatggat gagacatgga ataacttaga
      361 attaaaagcc agatgtcgaa agataagtat gagtttagga atgtatttac cagaagacta
      421 taaggaagct ctttccattc ttgaaaaatc agttacaggt ttttattttg cttttttctt
      481 tccagatttt gttgaagttt atgggcagga cgacataaat tgggatttat ctataagtgc
      541 attagaaaga aatactgagt attggtcttc agaatttgct gtaagagcat ttatcatcaa
      601 ggatgaagat cgtatgatgg cacagatgcg taaatggtct aagcataaga gcgaacatgt
      661 acgaagactt gcaagtgaag gttgtcgtcc ccagttgcct tggggacaag caatatccaa
      721 atttaaaaaa gaccctacac ctgtactgcc tatattggag caacttaaaa cagatacatc
      781 aacgtatgta caaaagagtg ttgctaataa tctaaatgat atctcaaaaa cacatccaga
      841 tttagtcata agcattgcaa aggattggta cggtaaaaat aaatccacta actggattgt
      901 aaagcatggt tgcagaactc tgctaaaaaa aggcaatcgt gatgtattag ccctttttgg
      961 atatgatgat actacttcta taaatcttca ggactttact ttagaaacaa cgtctatttc
     1021 tattggagag gatttaactt tttcatttaa tattttagca aaaaaagcga caaaaacaag
     1081 gctcgagtac ggaattgatt atattaaatc taatggaaaa aggaatagga agatatttaa
     1141 aatatcggag gtttcattaa aagaaaatga gaaaaagtca tatatgaaaa aacattcatt
     1201 tgcagatgta agtgtaagga gacattatcc aggcatccac tcgattgcta ttattattaa
     1261 tggtatagaa aaagataaac ttgattttga attaggtata taattttact aacagaataa
     1321 tctatatcag atactttgct aaacttatat aatatatttt actaaattta tattaggtag
     1381 tttactactt gtttactgaa tttaaataca aagaaataca ggtgttaatc cctgcccatt
     1441 aaaggaatat gagcataaca caaaagtaga acttatcaat tctgttacaa gtacagtagt
     1501 aaatcctcat aatcacaatt agcaaatgaa gtgtaaaaaa taagttagtg ttgattttag
     1561 ttgctagtga tattgaatag ttatacaata gccatataaa ttagaatttg tagaggagaa
     1621 atgtaaatgg atgtatatag tttttggaat gacactttgg cacaagatgc tgttaagata
     1681 agaaaatatt ttcatgaaga tgcttatatt aattggcatg atacaaatga gcattttact
     1741 ttagaagaat ttattattgc aaattgtgag tatcctgata aatggaatgg taaagtagaa
     1801 aaggtgctag aatttggaaa tttagtaatt acagtgacac atgtttatgc aataaataaa
     1861 ccactatcat ttcatgtcgt ttcatttatc agccttaaag atgataagat tatttctatt
     1921 gatgaatatt ggggagagga tgatgatgtg ccacagtgga gattagataa acatattgga
     1981 aagccaataa aatagtgttt gttctgtttt ataactgaaa ttttgataac atttgatact
     2041 ttgatttgtc attggtcaac atagtcgtta gtgtaataat ttaaaaaagt agaatttgga
     2101 gagtgagcac tatggaagaa aaatacatct tatctgaccg aggtaaagta tattattgga
     2161 taaaagaaag taatagtgtg tttgcaaaga acttaatttt tttgccagga ctaacagcaa
     2221 atcatcatct ttttgatttc caaatacagt acttttcaaa aaatcataat gtattggtat
     2281 gggacgctcc tgctcatgga aaatcaagac cttacttaga tttctcatat tctaacttgg
     2341 tagaagaatt gaaagtaatt ttagataaac aaaatatgaa aaaggttgta cttatagggc
     2401 aatcggcagg gggatttgtg gcacagtcct ttattgtaaa atatccaaat atggtagaag
     2461 gattaatgat gattggaact tgtccatatg gaactgacta ttatagtaag tcagatattt
     2521 tctggttaaa acagactgaa tggatgttaa agttattccc agataaattg ttgagaaaca
     2581 taatatctaa gatgtgtgga gctacgtctg aaggcagaag gagtatgatg aaaatgttag
     2641 aaggctacga aaaaaaagaa ctgtgtcatc tcatgtatct gggatttgct gggttcatac
     2701 cagaaattca agacttaaat atacattgtc cagtttgttt ggtggttgga gataaagaca
     2761 agacgggtaa agtgagaaaa tataatgagc agtggcacaa taagactggg tatccattgc
     2821 acattataaa aaatgcttca cataatgtaa atgtcgataa acctaatgaa ctaaatcaga
     2881 ttatggatat atttattaag caattataat atataaaaat tacataccca ttaggtaaat
     2941 tatgtagtat atgtgtttac agatggttat agaaataaat atgtaataaa ctacttaaca
     3001 cttaaaaagt ggatttgtcc agagcataat acaaagcatg atatatataa atttttaagc
     3061 gaaatacatc aaaagaagga ttagaattag tggagacaga acagctatta tagattataa
     3121 ataaatagtt ttaaaggatg ggtactacga atatagagga aggtaaatca tacgttattc
     3181 aaggattata aatgattagt ttaaaagtac tatcggatag gagtctccat atttaaacct
     3241 tagtaaaggt agttaaagta gtagtttaaa aactaaaaaa ttatataaag gaattaggta
     3301 taagaaaatg attgagagta taccagcaaa acaaatttta caaaaagtga aatttgacaa
     3361 tacaagatgg tttggaattg actataatat gaatctctat cgtggctgtt ctcatggatg
     3421 tatatattgt gatagccgta gcacaatata caacatagag aattttgaca aagttagata
     3481 taaagaaaat gtgatagaaa ttttaagtaa agaattgcgt tcaaaaagaa aaaaaggggt
     3541 agtaggcatt ggagctatgt ctgacacata taatcctttt gaaaagcaac tttgtattac
     3601 aaagcaagca ctagatttga tttcggaaaa tcattttgga gtatctattg atactaaaag
     3661 tagtttggtg gttagagata taccgatttt gcagaaaatt aaaaaaaata attctgctat
     3721 tgtaaagttg actataacca ctgcaaatga tgaattatca aagaaaatag aaccttatgt
     3781 aaatccaagt tctgtacgat ttgacgcagt gaaagaactt aatgattctg gaattttttg
     3841 tggcatatta ttgactccta tgcttccatt tttgactgat aataaagatg aaatacgagc
     3901 aattgtagaa aaagcacaca aggctaatgc aaaatttatt tattgcatgt atggcgtaac
     3961 aatgcgtagt ggtcagagag agttttttta tgagcattta agagatatat caccaaaatt
     4021 ggtatttaaa tatcagaaaa catatggttt aaactatgta tgtactgtac aaaataaaga
     4081 ttgttgtgag aagttgttaa gagaagaatg tgcacgttat ggtattttga cggatatgaa
     4141 agagattata aaagcgtaca agaggtcgga gtcatatatt caaataaaat ttttttaaga
     4201 taggctttgg atgagatata atagtggtga ttgatgtgtt gatattatag ttgattgtta
     4261 gagaggatat tatagatgaa tattagtatt gatattctag gtaattgcta gagctgatat
     4321 tatagatgaa tattaatatt gatattctag gtaattgcta gagttagtat tatagatgaa
     4381 tattagtatt gatattttag gtaactgcta gagctaatat tata
//
LOCUS       DAKBNN010000263         4422 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000263 DAKBNN010000000
VERSION     DAKBNN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.269"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            411..1718
                     /locus_tag="OUB02_003581"
     CDS             411..1718
                     /locus_tag="OUB02_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887303.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1804..2066
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2192..2995
                     /locus_tag="OUB02_003582"
     CDS             2192..2995
                     /locus_tag="OUB02_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCT8887304.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPNNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3033..3644
                     /locus_tag="OUB02_003583"
     CDS             3033..3644
                     /locus_tag="OUB02_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCT8887305.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3676..4347
                     /locus_tag="OUB02_003584"
     CDS             3676..4347
                     /locus_tag="OUB02_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCT8887306.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1773 a          410 c          807 g         1432 t
ORIGIN      
        1 agtacaatag tgttaatgtg tgaattagat aaggtgtata taatagttta gatgatatga
       61 tataaaatat gttgtaaatt aaatgttttc tttgacaata tatacacaag gatgataata
      121 tctatatgaa taaaagatag tgtgtaattt gataatatgt aattataaca ttatgatata
      181 attacaataa agtatgtaat aatctgtaga aatttcaagc tagttatcta agtcggataa
      241 ctaaatctaa acggaataca taatgtatta aataaatgat gatactaaaa tgatactatt
      301 tttatagaaa tttattgagg tatataatta atgaaaataa tatattaaat tatataaatt
      361 aaattatata atatattgag ttttacataa aagcaaaggt gggaataaaa atgaaagaaa
      421 ttgctgatgc tataacatca gagtatttaa gctatggtat caatgaagat attagtatct
      481 atgaaggtag attttgcata tatttagata aaaaatatag atgtaatggg aaaatttatt
      541 ataaaatgac acctccaata tctataagtt ttaaagcaga cataggttgt gttgaagaga
      601 tagacaatga agatgacaat ttagctttag attatgataa tgctattctt gaagttcatg
      661 gatataaaat tatttctata acaataaata ctttaagtga atttagtgta gaaggttata
      721 tcaatgatga ctgcataaaa tctaagaact cctatgttga atatgtagat tttaatataa
      781 taaacttaga taaaatacct ggaaaactca ttaaatacaa tgataaggta tacgcaggta
      841 gaattgaatt tgatataaat gattatgttg taactataga caagcgatat gattatagaa
      901 aagaattaaa atcagaatta aagtctaaat ctggagcaat aataactcat ataggtagga
      961 ttagaagaaa agatggtaga atttttagaa caaataatac aataaatctt ttagatagga
     1021 tatcaacagc tttgagtttt atgtgtggaa gatatgtagg gttttgtttg gccaaagggt
     1081 atagaagtgg taatgaagtt tatagaatat ggaatgaaaa tcaaatatct ccttttagat
     1141 atgtaccaac atggtcagat acactttcaa attatcataa tatggaaaaa tatataagcc
     1201 ttatgtgtaa aaagctagaa gatttttatt atggttctgc tataaagagt gttgttgatt
     1261 ggtacataga atctcttgga agtgctacca tggaaaacaa tataatatct gtacaaattg
     1321 cacttgaaac tttgtcatat gtaattctag tggaacaaaa taagatactt acagatgaag
     1381 tatttgactg taatttggca tctaaaaata taagactctt acttgataca tgtaagatac
     1441 catatggtaa acatgaatta aatatatttg ataatataat aaaaaataaa tttgatgatg
     1501 gagtggattt agttatttat ttaagaaata gcatagttca tccaagtaga aagactcata
     1561 gagctgtgtt agaggttgaa gatatttgga atataatttc aataggaacg agatatattg
     1621 aacttgtttt actctttatt ttaggttata gaggagaata ttcaaacaga ttagtagaaa
     1681 gatgttatgg tgaagtggaa gttgtacctt ggaactagat ttttatattt attttaaaat
     1741 atatattgac atatattgcc ttatgctata aaatcaatat cataataata attcagaata
     1801 acattaaatg ctaagaaaag aaatagtaaa agtagttaat cttaaacaga gagtttccga
     1861 ttggtgagag gagcaataga ttttactttg aacacatctt tgagcaggac tctgaaagag
     1921 tagaaatact ttattaggag atttccggaa tccttgcacc cgttatagtg ctagagtata
     1981 ataattttat tgtacttaaa gaggttagtg tcgtgaggca ttaatgaaca gaggtggaac
     2041 cacgagagtt aactcccgtc ctctagcaga agctagaggc gggagttttt taatataatt
     2101 ttttaagcgc gcagagaatt atattgcaat agatacactt tttaaaaaat aatcaaaata
     2161 ttttaaaaat taaaagacga gggagaatat tatgaaaaat atattaaaaa aagtaggaat
     2221 atttacaatt atgttaggtt tattaggtgg agttgtaggt tgttcaaaac caaataatga
     2281 aaaagataag gatgcttcaa aggaatctaa aaaagaagta gttgtagggt ttgataatac
     2341 gtttgtacca atgggatttt tagatgaaaa gggagataca gtaggatttg acgtagactt
     2401 agctaaagaa acatttaaaa gacttggaat ggaagttaag tttcagccta tagattggtc
     2461 aatgaaggaa actgaattaa atgattcaaa aactgtagat gtattatgga atggatattc
     2521 tataacagac gaaagaaaga aaatagtatc atatacagaa ccatatttac aaaataaaca
     2581 gataatagtt actctaagtg actcaaaaat aaattcaaaa gctgatttaa aagataaaga
     2641 agttggaaca caacaaggtt caacagcact tgatgcagtg gaaaaggata aagattttat
     2701 gaatagttta aaaggtggag caccagttct ttatgatact tatgataaag cacttagaga
     2761 tttagagata ggtagaacta gtgcagttgt tggtgatgaa gttttaatta gatactatat
     2821 gggacaaaag ggtgaagaca aatacaaggt attaaaagac gattttggat tagaagatta
     2881 tgttgtagca acttctaaag aaaatccaga actttgtgag aaaataaatg aaacattaaa
     2941 agaaatgaaa aaagatggaa cttttgacaa aatttatgat aagtggttta aatagaagat
     3001 aagttagcag ttaagaaggg ggcactaaag gcatgttaca aggtttagag atagttatag
     3061 caatgttttg tataacattg atagtatcaa ttccattggg gattggagtt gcatttttaa
     3121 gattatcaaa gaataaatta gtaagtggta ttacacaatg ttatatattg ataatgagag
     3181 gtacaccttt acttttgcaa atgatagtca tattttacgg attacctctt ttaggaattg
     3241 tatttgacag atttacagct ggagtagtag ctttcttctt aaattatgca gcatattttg
     3301 cagaaatatt tagaggtgga atacagtcaa tcgatagagg acaatatgaa gcttcaaaag
     3361 tactaggatt tgataagttt actatgtata agagagtaat ctttccacaa gtttttaaaa
     3421 gaatactagc acctatttca aatgaagtaa tcacattagt aaaagataca tctttagtat
     3481 atatattagg attaaatgat attttaagaa tatctcaaat agcaatgaac agggaagcaa
     3541 gtctgttacc attatttgag gcaggagcga tatatcttat atttgtagct atacttacta
     3601 agggatttga attacttgaa aaaaaatatt cttattatag ataaagttac aatattatag
     3661 gaggaggttt tttatatgtt gaaaatcaaa aacttaaata aatcatttaa aaaaaataga
     3721 gtacttaaag atatttcttt tgagttagaa gaaggtcaga ttggagtttt acttggtaag
     3781 tctggtgcag gaaagaccac aatattaagg tgtataaatg gtcttgaaga atttgatagt
     3841 ggagagatta taatagataa tgaggtaata aaaaataaga gagatatggc aaaaatacga
     3901 ggaaaaatag gtatggtttt tcaaaatttc aacttatttc ctcatatgac agtccttgaa
     3961 aatataattg aatccccagt taatgttttt aaagttccta gaaaagaggc tgaagagagg
     4021 gctagagaac ttttaagatt agttgattta gaagataaat taaattctta tccatttgaa
     4081 ttgtcaggag gacagcaaca gagagttgct atagctaggt catgtgcatt gatgccaaaa
     4141 gtcctttgtt ttgatgaacc tacttctgca cttgatattg atactattca aagagtagtc
     4201 aatataatga acagacttaa agacaaaggt atgacaatat taataattac acatgatgtt
     4261 gtcttctcaa ataatgttgc agataaaatt ataagtataa aagatggtat agtagaaaat
     4321 gttcaaataa aagaaaaaat tgtttagagt tttataaata taaactaggg tagaatttta
     4381 taatataaat aaagggcaag taaaatcaaa tatactatgc cc
//
LOCUS       DAKBNN010000264         4409 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000264 DAKBNN010000000
VERSION     DAKBNN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.270"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(138..1382)
                     /locus_tag="OUB02_003585"
     CDS             complement(138..1382)
                     /locus_tag="OUB02_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCT8887307.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            1710..2498
                     /locus_tag="OUB02_003586"
     CDS             1710..2498
                     /locus_tag="OUB02_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCT8887308.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            2498..2971
                     /locus_tag="OUB02_003587"
     CDS             2498..2971
                     /locus_tag="OUB02_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCT8887309.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(3063..3503)
                     /locus_tag="OUB02_003588"
     CDS             complement(3063..3503)
                     /locus_tag="OUB02_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HCT8887310.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(3518..3811)
                     /locus_tag="OUB02_003589"
     CDS             complement(3518..3811)
                     /locus_tag="OUB02_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCT8887311.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(3854..4255)
                     /locus_tag="OUB02_003590"
     CDS             complement(3854..4255)
                     /locus_tag="OUB02_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887312.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
BASE COUNT         1373 a          758 c          558 g         1720 t
ORIGIN      
        1 gttcgattcc atacaaaaaa gtgcctcata aactaaaaag ttcattttga gacacctttt
       61 ttatatctaa atatattcaa atacatacac aatcattgta attatttaaa tgtattttat
      121 ttagttgttc tttataatta atccataata ttatattctt catataaagg atatttttca
      181 gttaaaccaa caaccaatgt tctagcctta gattcttcat cataagttaa acataggtct
      241 atagcctcag cgattattgc catatcttct tctttcatac ctctagtagt tacagctgga
      301 gtaccaagtc ttattccact tgtaacaaga gcaccatttg ggtcaaatgg aattgtattt
      361 ttattagctg ttatataagc atcatccaat ctcttttctg ctgctttacc agttatattt
      421 ttattagtca aatctaatag tattaaatga ttatctgtac ctccagaaat caatttaaaa
      481 tctcttttaa taagttcttc agctaaggct tttgcatttt tagcaacttg tacctgatat
      541 tctttaaact catcacttaa agcttcttta aagcaaactg ccttagaagc aattatatgt
      601 tcaagaggtc caccttgaat accagggaat attgctttgt ctatgtcttt agcatatttt
      661 tctttacaaa gaataacacc cccacgaggt cctcttaaag ttttgtgagt tgttgttgta
      721 acaaaatcag ccacttcaca tggattttgg tgaagaccag cagccacaag acctgctata
      781 tgagccatat ctaccataag caatgctcca acctcatcag ctatctctct aaacttttta
      841 aaatctattt ctctaggata tgcacttgct cctgcaacta tcattttagg ttttacttca
      901 tgtgctatct ttctaacttc atcaaaatct ataagtccac tgtctttatc tataccatat
      961 tcataaaatt tataatattg accagaaata ttaactggag caccatgagt taaatgtcca
     1021 ccttgagata aattcattcc cataacaaca tcacctggtt ttaacatagc aaaatataca
     1081 ccaatatttg catttgctcc agaatgaggt tgaacattag catgttctgc tccaaatatt
     1141 tttttgattc tatctattgc aagagtttca acttcatcta tgtattcaca tccaccataa
     1201 tatcttttgc ctgagtaacc ttctgcatac ttatttgtaa gatgacttcc catagtttcc
     1261 attactggaa ctgatactat gttttcagat gctattaatt caatatttct ttgttgtctt
     1321 tttaactctc ttttcatact ttcataaatc tcagggtccg cttttttcaa gttctcaaac
     1381 atattactcc tcctagttat tatttaaatt ttatttttac agacatttca taattttcgt
     1441 ttttatcaat atgataattt attattatag tcctttacca attaatagtc aagtgttttg
     1501 ccatttttcc atatttaaca tagttacttt ctttattttt aagttaaaat ataataaata
     1561 atattgtcta attcttttaa ttggatataa tttaaatgtg actattttga aacaaaaaaa
     1621 agataatata tttatataga tattaaggaa tattacttaa aaatttgata taatttcaat
     1681 attacgtttt ttaatttgga ggtgtattta tgaatcaaaa tgaggaactt gaatataaga
     1741 aaaatattct aaaactagct ctatttgtag gggaactgat gataaaaaat ggagctgaaa
     1801 catctagggt tgaagacagt gtccttagaa tatgcaggtc aagagggttt tatcatgtaa
     1861 atatttttac aactcctaca gtaataatta tttcagatga gaaatttgat ggcttttcgt
     1921 ttatgaaaac gattcaatca agaggtataa acttaaataa gatttcttta ttaaacagtt
     1981 tttctcgaga atttgttagt gacaaagagt atgatattga taatgccatt gcacgtcttt
     2041 atgaaatcca agatgtaaaa ccttatcctt tatggttatt ttactcttgt acaggaattg
     2101 catctgcttg ttttgcttgt ttgcttggag ggaattatgt attaaatttt atacttacgt
     2161 ttataatagc aatctttgct gcaattactt atgataaaac gatgaagata agtgcaatat
     2221 ctgcattttc ctgtttagta tcttcttttt taattgcact ttcaggagtt ctactagttg
     2281 aaataggcat tttagatgac cctaaaatgc ttatagtcgg cgctataatg ccactactcc
     2341 caggagttgc ttttataaaa gctattagag atttaatatc agggaattta atatcaggaa
     2401 tttctcgtgc ttttgatgct gctatgatta ttattgcaat tgcttctgga gttggttttg
     2461 tccttgatac atggtataga ataggaggtc ctttctaatg actgatatgt ctatgatttt
     2521 gcatttttta ttttcaggtg ttgcaactgc tggatttgct gttttcttta atgctccact
     2581 atatctactt cttccagctg gagtaactgg cggaattggt tggattgtat atgtttattt
     2641 atttaatttt actacaaatg cagtttttgc aggttttatc gctgctgctt tggtctcagc
     2701 gtgcagtgag actttagcaa gaaaactaaa gcaaccagct attgtgttcg taattccagg
     2761 tattcttcct ctaataccag gaattgggct atataataca atgttgtctt tgattcagaa
     2821 aaattattct ttggctatgt caaaaggtac agatgcgcta tttctaagtg ctgcaatagc
     2881 acttggtgta ttagttgtaa cttcttttgt aagaacattg aatcttttga aaatcagaaa
     2941 aaactttatt ccttacagaa aatcaaaata aaaatataaa aatagcaatg tacttaaact
     3001 ctacaaatac gtattgagta tttatttaaa aagcacattg ctatttttta attataatta
     3061 tcttaaagat caattaatat tcttgttatt actggtataa attctctact gtctctaaat
     3121 tttttgtcat tcttctttaa gtataaataa cagattattg taagacaaat tccaactaat
     3181 ccacttatgg cttcaactcc agttgtcatt ccaattgccc ccaatatata ataagtccct
     3241 attgttccta caagaagtgc tataagaggt attatataag ccattacagc tgcttttaat
     3301 acattcatat tatccatact cacttctaca tggtcaccaa cttttgcacc aattgtgttg
     3361 tctacttcta ctaatatttc ttttgactct gctgaatttg tagtagcaca ttttccacaa
     3421 gcagcacaag ctgaatgtct ttgcattttt aactttgcag ttctatcatc aacaatttcc
     3481 ataataaatc cttgttgatt cataactaac cgcctcctta ttttgtaaat ttatttatag
     3541 tatccataag ctcttttatc atctcttcag ttttatcagc atcatcatgt ttaatgcttt
     3601 ctgctacaca tccttttata tgattctcta agataattgt gcctactcta tttatagcag
     3661 accttatagc agatatttga accaatatat caacacaata acgttcttgt tccaccatct
     3721 tttgtatacc cttaacttga ccttctattc tttttaatct ttttatgaga gcttccctct
     3781 cattgtttgg tgctaatttt ctttcttcca taaactcacc tcatactcta atgatgtctt
     3841 atacaattat gctctactta ccaacttgtt taaaatataa tacagttatt attactataa
     3901 atgccaaaat tgatattgca atagacctac tttgattctt agtttgttcc atacttaact
     3961 ttccattcat cttaaatcct gccaatggga ataaaaatat aaccagtgca tcaattccaa
     4021 gtacaacttg ggctgcaata gtccatttac tcaaaaagca gaatattccg cccacaccta
     4081 aaagaacctc taatatattt ataaacttat atgtaggttt ttttgctata tattcttcaa
     4141 ttttttcctt acattctgca cgacatggat atatattcat attgtcatca tattttatag
     4201 ctaatttatt atttggtgac tttatatccc tattacagta aagacatttt ttcattttat
     4261 attctcctct agtcttcctt aaaaaatttt gcctaaatta ttatacgctc taatactatt
     4321 ataccaaata gatacaaaaa acaaaaataa aaggaataat tttagctact tcttttactt
     4381 caatgtgtag tctataataa atttataat
//
LOCUS       DAKBNN010000265         4285 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000265 DAKBNN010000000
VERSION     DAKBNN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.271"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            528..1436
                     /gene="trxB"
                     /locus_tag="OUB02_003591"
     CDS             528..1436
                     /gene="trxB"
                     /locus_tag="OUB02_003591"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCT8887313.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1526..3349)
                     /gene="typA"
                     /locus_tag="OUB02_003592"
     CDS             complement(1526..3349)
                     /gene="typA"
                     /locus_tag="OUB02_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCT8887314.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3561..3803
                     /locus_tag="OUB02_003593"
     CDS             3561..3803
                     /locus_tag="OUB02_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887315.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3951..4142
                     /locus_tag="OUB02_003594"
     CDS             3951..4142
                     /locus_tag="OUB02_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCT8887316.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1572 a          551 c          680 g         1482 t
ORIGIN      
        1 ttaatttgaa aaatgtaatt atatggtata tcatataaat ataagtaaat agtgctaata
       61 tttaaactat taatcataat tggttcaaca aaagtatata gttaataatg tttaatttga
      121 agaaattgtt agtgtgaaat tacatatata ttcaaattaa attcataaat atttcacata
      181 ataatagtat actgtaaaca aaaggaggta ttaaatatgg gaatagtact tataacagca
      241 gcattagttt tggttatgta tggactttca attctatcat atatatcaga aagcaatata
      301 ttagaagaca ctgtaaatga taaccaataa ggttttgaat tatataaata agtgagaact
      361 gtaaataaaa gttaattttt atttgcagtt tttttatata tatgtaaaat aaagttaatt
      421 aataaaaaat aatgttttgt ttatagtata tatggataat aatagtaaag tataagaatg
      481 atgagaataa tatataagaa ataataatat aaagagaggt aaacgttatg gtagatatca
      541 ttgtaattgg tgctggacca gcaggtttaa cttcagcaat ttatgctatg agagcagggt
      601 taagcgttac agtatttgaa aaaaatattt atggaggtca agttgcaagt acctctgagg
      661 tagaaaatta tccagcagtt caaaagattt ctggggtaga attttctaat aatatataca
      721 atcaagcagt tgctcaaggt gttgatattc agtttgatga ggttgaagag ataaatttag
      781 agggaaaagt aaaggttgta aaaacttctt caggtgaaca taaagcaaag gctgttattc
      841 ttgcaaatgg agttgaaaga cgtaaattag gttgtgcagg ggaacaagaa tttacaggta
      901 gaggagtatc ttactgtgca acatgtgatg gagccttttt taaagataaa gaagttgcta
      961 tagtaggtgg aggaaatact gcactagaag atgctttatt tttagctaat aattgtacaa
     1021 aagtgtattt aattcacaga agagatagtt ttagaggtga agaagtgttg gagaaatctg
     1081 tgaaagcaag agagaatatt gagatacttt acagtcatgg tgtagaaaaa atagaaggag
     1141 aaaagactgt atcgaaaata gaagtaaaaa acttaaagac tgaagaaaaa agaactatag
     1201 atgttagtgg tatttttatt gctataggtc ttaaacctaa taataaaatg tttgaaaatg
     1261 ttcttgattt ggatgaaggt ggatatataa tttcagatga gtcttgtaca acatcagtag
     1321 aaggtgtata tgtagcagga gatagtagaa ctaagttttt acgtcaaata ataacagcag
     1381 catcagatgg agctattgca gccgttcaag cagctaatta tataaatgtt gaataatata
     1441 aatttcaaat aaaaatagcc atataaactg aaataatttt tcagtactat atggctattt
     1501 tgttaattag aggtaaatgt acgttttatt taccttgtct agatttgtta tatcttaatc
     1561 tatcatgttc atttaagaat ctttttctaa gtcttataga atcaggtgta atttcaacta
     1621 attcatcatc ttctataaac tctagtgctt cttcaagagt aaatattcta ggaggagata
     1681 attttatagc atcatctgaa ccagaagcac gtacgttact cattttcttg tttttacaag
     1741 ggttgactac catatcatct tttctggaat tcataccaat tatcatacct tcgtaaactt
     1801 ctacaccagg gtctacaaac attacagctc tatcacttaa ggcattaaga gaatacccca
     1861 tagtaacacc aggtccttgt gctattaaaa caccatttcc tcttgaaggt atttctcctt
     1921 taaattcttc aaatttttca aatgaacgaa ctaaagtacc ttctccacga gtagcgttta
     1981 taaattcact tcgatatcct aaaagaccac gagtaggtgc taagaactct atttttacat
     2041 agtctccttc tattgacata gactccatca taccttttct catgtttaat tcatttataa
     2101 tagtaccaga gtaaacttca ggacagttta caacaactct ttctattggt tccattagtt
     2161 ttccatcttc tttatgcatt aaaacttcag gtttagaaac accaacttca taaccttcac
     2221 gtctcatatt ttcaagtagt atagataagt gaagctctcc acgtccagaa actttgtatc
     2281 catcagtagt atcaagaggt tcaactttaa gaccaacatt tacttcaagt tccttttcaa
     2341 gtctatcttt caaatgtcta gttgtaacga attttccact ttttccaacg aatggagagt
     2401 cgtttactaa gaagttcata gaaagagtag gctcttctat ttttatcatt tccataggta
     2461 gtggactatc taaatcgcat atagtttcac ctatagatat atcaggaata ccagctataa
     2521 caactatttc tccactagta gcttcatcaa cttcaacttg ttttaaacct tcatacacag
     2581 aaagttttga tacttttcct ttagatacaa cagaatcttc tctacatata gcaacttgtg
     2641 tattgttttt aagagtacct ttgtaaattc tacctatacc aagtctacct acataatcat
     2701 catatgcaag agctgatatt tggaactgta gaggttcatt atcgtaatta ggataagctt
     2761 caacgtgatt tacaatagtt ttaaatagag gagaaaggtc ttcactatca tcatccattt
     2821 caagtttagc aataccttgt ttagcaatac catatattat aggaaattcg cattgctcat
     2881 ctgttgcatt taagtctaca aataaatcga aaacttcatt aactacttct tccgctctct
     2941 ggtctttttt atcaatcttg tttataaata atattggttt taaaccgaac tctaaagatt
     3001 tttgtaaaac gaatctagtt tgaggcattg ggccttcact agcatcaact aatagaataa
     3061 ctgtgtcaac agttttcata acacgttcaa cttcagaaga aaagtcactg tgtcctggag
     3121 tatcaactat gtttattttg taatcttcat agtttattgc acagtttttt gaatatatag
     3181 ttatgcctct ttctttttca aggtcgttac tgtccataac gcagtcttta actacttcgt
     3241 tctttctaaa aacaccgctt tgagaaagga aagcatcaac caatgttgat tttccagcgt
     3301 caacgtgggc tataactgcg atatttataa ttttatgttt ttgtgacatt tttgttgctc
     3361 ccttcgatta aaataactta ttaattatag cacaatatat tattaggtac aaggaggaat
     3421 atattaaatt gtattaatat agtttaaatt ggataaaact gttagttttg catctgaaaa
     3481 ttattctgtt taaaaaaatt ttttagacaa ataataacct taatagataa aaagattaat
     3541 aaaagagtaa aggatgatat atgttggaag taaccaaaat aataattaaa tattttgcaa
     3601 gtgaaagtga tacttttgta aaagaagaag tttgtaattt tgaagaactt cttaagtggt
     3661 ctcataaatt tattgaaggt aaaaagatag aagatgtttt gtatataaag tctaaagaaa
     3721 actataaagt aaatgtgatt aagtctttag aaattaagga attggaaata gtgagaaaac
     3781 aacagatacc accgtacatg tagtattaat aatatataaa attaatatgt aatactcgca
     3841 ataggatata aatggtatat atggtaataa tttatattaa aaaaactttt ataaagttta
     3901 tatttgggta taaaagtatt aattagacat taattaggag gaacttaaat atgataagag
     3961 tatgtccaaa ctgttcaaat gtggatgtaa ataagctaaa aactttggtt ggagaagaaa
     4021 atgtaaagac tggttgtata ggtcagtgta gagcatttaa aaaagaagct gttggaataa
     4081 tagacaatga gcttgaaata aaggaaacag aagcagagtt ttttgaagca tgtaaagaat
     4141 aaattttaaa taaaatataa taaatagagc atctaaattg tataagtatt aaacaattta
     4201 gatgctctat ttttatatat acatttaatt atgagtatac ttttctatta aatacaaata
     4261 aatataacta atacatttat ttatt
//
LOCUS       DAKBNN010000266         4269 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000266 DAKBNN010000000
VERSION     DAKBNN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.272"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            340..2439
                     /gene="nrdE"
                     /locus_tag="OUB02_003595"
     CDS             340..2439
                     /gene="nrdE"
                     /locus_tag="OUB02_003595"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCT8887317.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEAFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2452..3423
                     /gene="nrdF"
                     /locus_tag="OUB02_003596"
     CDS             2452..3423
                     /gene="nrdF"
                     /locus_tag="OUB02_003596"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCT8887318.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3499..4126
                     /locus_tag="OUB02_003597"
                     /pseudo
     CDS             3499..4126
                     /locus_tag="OUB02_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
BASE COUNT         1707 a          458 c          786 g         1318 t
ORIGIN      
        1 tatattttta agctatttag agataattta aatttttaaa caggacataa taagcttgta
       61 ttgttttata tttgagttga taaaaatttc tttaaataaa aataatgtca attataaagt
      121 aataaaagtt tagatataaa aaaatttgtg tatatactat atgtagtata taatagataa
      181 agcactgact atatgtagtg cttttgtttt tgaaaaatac ttattgcatt tctataatta
      241 tagattaatt ttgggatttc aactgataag taccaaaaga aataataagc aaattaagtg
      301 aaattatggc acaagattat gttaaaagga cggtaaataa tgaattggat agagcttaac
      361 aatcaagtga taattaaaaa tgaacaaggc aagtaccaat tggaaaagga taaggaagcc
      421 ttaagtagtt acatagaagc atttatacag ccaaagttga gaaaatttaa taacctagaa
      481 gaaagattga agtatttaat agatgaaggt tattattcaa aagaagtaat aaatcagtat
      541 agcatagatg ttattaaaaa tatatatgat atgattgata aacataagtt tgaatttcaa
      601 tcatatatga gtgcaaataa gttttatcaa aattatgcat taaagagcaa tgatggaaag
      661 gaaatattag aaacatataa tgataaagtg ttaattgtag ctttgacact aggaaatggc
      721 gataaagatt tagctttaaa cttggcagat aaaatagtaa aacaagaatt tcaaccagct
      781 acacctactt ttttaaatgc tggaagaaaa agggctggag aaatggtatc atgtttttta
      841 ctatctgtag aagatagtac agaaggtata tcgtatgcta tatcttcatc aaatcactta
      901 tcaaagattg gtggaggagt tgcactaaat ttatctaaac ttagagcatc tggcgaatct
      961 atcaaagata tagaaggtgc agctggaggt gttgttggag tagctaaaat gttagaacaa
     1021 tcatttagtt actttaatca aatgggagct agacaaggtt ctggtgcggt gtaccttact
     1081 gtattccatc ctgattttga attgttgatg gatactaaaa agataaatgc agatgaaaaa
     1141 ataaggcttg ctacattatc attaggagct ataataccag ataaatttat ggagttggca
     1201 gaaaaaaatg aagtagcata tgcattttat cctcatactg tgtataagaa atatggagtt
     1261 tctcttgatg agatagaaat ggatgagtgg tatgataaat tggttaatga tagtgatata
     1321 agaaaaaaag aaataaatcc taggcagatg ttgacaaaaa tagcacagat gcaacaagaa
     1381 agtggatatc catatgtagt gtacatagat acagctaata gagaacatac attaaaagat
     1441 gttggaatta ttaaaatgtc taacttatgt tgtgagatat ttcaatatca aacaccttca
     1501 gaaatagagg gatatggagg aaaaaatgaa tggggacaag atataagttg taacctaggt
     1561 tctttaaaca tagccaatgt aatggataat aaaactatag aaagtacagt tgaaacagca
     1621 ataagagcac tgtcatttgt agcagatagc acagatataa aaccagtgcc aacggtaagc
     1681 aatagtaaca gcaagtctca ttctatagga ttaggagcta tgaatttaca tggatactta
     1741 gtaagagaaa atattttata tacttcagat gatgctatag atttttctaa tgtatttttt
     1801 gcaatggtta gatactattc aattaaagct tctatgaaaa tagctataga aagaaatcaa
     1861 acttttgaag gatttgataa atcagaatat gttaagggaa gaaatagcaa ggttttatca
     1921 aaatactatg aacaatctta tctacctaag tcagaaaagg taagagcatt atttgaaggg
     1981 atttatatac caacaaaaga agactggaca aaattattgg atgaggtaaa agaaaaaggg
     2041 atatataatg catatttaat ggcagtagca ccaactcaaa gcattagtta tgttcaaaat
     2101 gctacatcaa gtataatgcc aattactgag ccagtagaag taagaacata tggagactct
     2161 actacaatat atccaatgcc atttttaaca aatgataata tgttgtatta tcaatcagca
     2221 tacaggatgg atatgagaaa ggtcatagat ttagtggcaa cagttcaaaa ccatgttgac
     2281 caaggtatat caacaacact gtttgttaca gatgaaaaga caactagaga tatagctaga
     2341 cattacatat atgcctataa aaaaggtttg aaaagtttat attacacaag aacaaagatg
     2401 acaagagata ctcatgagtg tctagtgtgt tcagtatagg gagagaaata tatgacattt
     2461 aatttacata aaatacataa tgcagttaac tggaatagag aagaagatgg atttacacaa
     2521 gctttttggg aacaaaatgt aaaacaattt tggcttcctg aagaaatatc tgtttctaaa
     2581 gacataaagg tatggaatga actaagtaat aaagaaaagg aattatataa aaaagtattg
     2641 ggtggtttaa cattactgga tacaaaacag ggaaataatg gaataccatc tatgatgagt
     2701 ttaacagaaa acctacaaag aaaagctgtc ttgtctttta tggggactat ggaagaaata
     2761 catgcaaaaa gttattcttc tatatttatg actctacttt ctaatttaga aatagatgaa
     2821 ttatttgaat ggatagaaac agaaccaaca ttacaaagaa aggctgattt agttttagct
     2881 caatacgaaa atactacaaa tcaagaggga ttatacctat caatggttac aagtgtattt
     2941 ttagagagtt tcttgtttta ttcaggattc ttttatccat tgtatctatc tggtcaagga
     3001 aagatggtag ctagtggaga aataatatcg cttattctta gagatgagtc tttacatggt
     3061 aagtatatag gattgctagc acaagaaata tacgattcat ttgataaaat ggataaggag
     3121 atgttagaag agaagatgta ttctatactt tatagcctta tggagaatga gatagagtat
     3181 acaaatgtta tatacagaga aagtgggctt gaaaaagagg ttgtaaactt tttaaaatac
     3241 aatgcgaata gagctttaga aaatctaggg tttgagaagc tatatactgt agatgctata
     3301 aatccaattg tattaaatgg tcttagcact gaaaccaaaa cacatgattt cttttcaact
     3361 aagggtaatg gataccaaaa aggagtgtat gaggaattag aggatgaaga ttttataata
     3421 taatttataa ttatgacaca ctgttgtcac ttttagcgtg ataatatcaa tgtataaaat
     3481 atatatggag gtatttttat gaaatatgaa tggagaaaaa gagataaaga gttatattta
     3541 cctaaaaaga atcctgtaat agttgaaata ccagaacaga aatttatcat gttaaaaggg
     3601 agtggagacc caaattctaa agaatttact gaagcaatag gagtacttta ttctttggca
     3661 tatgcaataa aaaccatgcc taaaaaagga attgtaccag aagggtattt tgactatacg
     3721 gtttttcctt tagaaggtat atgggataag ggagaaaaaa gtaaaggctt agatgtactt
     3781 attaaagaag atttaattta cactataatg atacgtcagc cagattttgt tacacaagac
     3841 ctgttggata aagctactga tatagtaaag aagaaaaaac ctcataatct tcttgaaaaa
     3901 gttagatttg ggtctattaa tgaagggcta tgtgttcaaa tgcttcatat aggttcatat
     3961 gataatgaac cagaaagctt tgacattatg aacgaatttt gcaaaaaaaa ataaacttat
     4021 aagaaaatct tacacacatc gtgaaattta tataagtgat gctagaaaag tatcgcctga
     4081 atcattgaag acggttttac gttttgaagt agaaagggaa gtataaatat tttgaataat
     4141 ttataagagt tataaaaagt gaagtctgtt gagacttcat tttttgatgc aattttaaaa
     4201 ttaaatttga tgtttcataa tattttaaaa actttctact tttattagtg tgaataactg
     4261 ttgctatta
//
LOCUS       DAKBNN010000267         4253 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000267 DAKBNN010000000
VERSION     DAKBNN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.273"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            199..765
                     /locus_tag="OUB02_003598"
     CDS             199..765
                     /locus_tag="OUB02_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8887319.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            complement(956..2992)
                     /locus_tag="OUB02_003599"
     CDS             complement(956..2992)
                     /locus_tag="OUB02_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HCT8887320.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDENYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3166..3843)
                     /locus_tag="OUB02_003600"
     CDS             complement(3166..3843)
                     /locus_tag="OUB02_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCT8887321.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
BASE COUNT         1473 a          664 c          477 g         1639 t
ORIGIN      
        1 aagaattatt aattcctcat agaagttatc attggtacat agaagttttt acataaaatt
       61 atacaataat ttaattaatt aatagttaca taaaaaagta acactttaat atattttgac
      121 accatattga cattagaatt aattcatgat attctgtttt cgacaaaaca acatctaaaa
      181 ataaaggaga tatatttcat ggattattca aatgaactaa aagagctctt tttaatgaat
      241 caaacatatg caactctttt tacccttaca aacaaaatac aaatagaagg agataaatat
      301 tttggtatac ttacttcaag acagtatatg actattttat caattcttca tttaccagag
      361 gaagaaacaa cattaaataa tattgcaaga aaaatgggta caagtaaaca aaatataaat
      421 agactagttg ctaatcttga aaaaaatgga tatgttgatg taattccaag tcctcatgat
      481 aagagagcaa ttaatgttaa agttacagac ttaggaaaaa aggttatggt gacatgtagt
      541 aggactggaa taaactttat ggcagatgta tttcacgaat ttaccaaaga tgaattagag
      601 accttatgga gtttactaaa aaaaatgtat cgttttaatg gtgaagaaca agatggcttt
      661 gaagaagatg ctaattttat ggaatatgaa gaaatagata aaattaaatc tgaagcactt
      721 gaagaatttg caaaaagaag aaagagagta aataaaaacg attaatacta tcgcatctat
      781 aatactactt ttttgtagta caatatttta tataactata atgtcatttt tattttttaa
      841 caaaacaatc attacataga ttaggtgttc ttaggaactc tttttctgtt ttttgtacaa
      901 aaaaaggaga tgattagact tttattttaa agcatctcct ttttcgctat tatgattatt
      961 ctactcctaa aatcatatct tttaattttt gtaaatcatt aattggttga ctacaactat
     1021 tactttgaca cacgtaataa ctagtcttat catccttcaa tatatagtta tttaaaaaac
     1081 ctataatagt gttttcttca ttgtatttct ttgctaaaaa agttgcattt ggtataaaat
     1141 tttcacttat caattcttta aatgcaatta aatcagaatc ttccttaaaa atacatataa
     1201 tttccttagt agaataaagt tcaaacatca atgaaagcat ataaaagctg tagccagttg
     1261 gagaactctt tacattatcc acatacagtt ttaattgttt atatgacatt tcttcaagtc
     1321 tattatctcc agtaattttc gcaagtctta taagattgta taattgaaca gaatttcctg
     1381 atggaatagc tccatcatac aaatcttttg gtcttgctat taaattttca ctatctttac
     1441 catatatata aaatccactt ttttcataat cccaaaaaag gttgatacag ctttcattca
     1501 aatttaaagc cttttctaaa tatttcatat taaatgttga ctcataaagc tctatatacg
     1561 cccagattaa aaaagcataa tcatctaaat atgctaaata gtcggaactt ccatctctat
     1621 atctagctaa taatctacct gactcattta caagattatt atttataaag ttaagacact
     1681 tatttgaata ctctaaatat atgtcatttt ttaaagtact atatgccttt gttaaagcaa
     1741 ctatcatcaa tgcattccat gatgtaagta tcttgtcatc tttatgcaaa gatgttcttt
     1801 cttttctata ttcaaatacc tttttactaa ggtcagctat cttttcatta tgtctttcat
     1861 attctttatt ttttataaga ttaggaatac ttttgccttc aaaatttcca ctactagtta
     1921 tatcaaaata attattaaag aaaattccat cttcttcacc caaaacttca attatctcaa
     1981 gaggattaaa tgtataaaat ttaccttctt caccttcact atcagcatcc tgtgctgaat
     2041 aaaacccacc ttctttatcc ttcatctctc taactacata atctatagtt tttatagcaa
     2101 tttctttata taactctttc tttgttattt tatacgcatc taaaaaagct atagtaagca
     2161 tagcattatc atatagcatt ttttcaaaat gaggagccaa ccacttttta tcagtagagt
     2221 atcttgaaaa cccaaatcca atatgatcaa acaggcctcc tctatacatt ccatctaaag
     2281 ttttttccac catttttaac acatctttat ctttttctat actataatat ttcattaaaa
     2341 acataagatt ctgaggactt ggaaatttag gagcattacc aaaaccacca taattttcat
     2401 cataaatagc cttaaatact ctaactgatg aactcaacat ctcttttgat aaatcaccct
     2461 cagtattttt tacaccaaaa tcatccttaa gagcttctat tatttcatct ccagatttta
     2521 ttaatatatc cctgcttgta ttccattttt cagatacatt ctctaataaa tctataactc
     2581 caggtctatt ataccttgaa tattttggga aatatgttcc cgcaaaaaaa ggttttttat
     2641 ctggtgtcat tattatggtc attggccatc caccactacc tgtcatagct tggcaaactg
     2701 tcatatacac gctatctaca tcaggtcttt cctctttatc tacttttatt gccacaaagt
     2761 ttctattcat tatttcagca acttcttcat cttcaaaaga ttctttttcc ataacatggc
     2821 accaatgaca tgttgaatat cctacactta aaaatattgg tttatcctct tcttttgctt
     2881 ttttaaaagc ctcatcattc caactatacc agtttatagg attataagca tgctgtaaaa
     2941 gatatggaga tttttcattt attagattat taggttttct attactgtcc aaaataccac
     3001 ctcatttgtt attttattat atgttttgca ataaacaaat agatatacac ttataataag
     3061 tgtataccca tttttaacta tacatattta atttttcata taattaattc ttctagtaaa
     3121 taatttttat aactactaaa acaaaacaac ataaaaaata tcaaattatt tttttaaagg
     3181 tttttcaaaa gaaggaggat taatatctat cttagtatcg tgatttggcc ctggagtaga
     3241 caaatcgaaa tataatttag aatcttctgt aactccaaaa tctctatttg aacctgtatc
     3301 tttaacttta tttaaagcat ctctaaatag agcattatgt gcttcttctc tattaagtaa
     3361 gaaatctatt gtttctttta catatttatc atctatttgt ctatacaagt actcataaac
     3421 taccttagct ctttgttcag aagctatatt tgaaagtaag tcagctacta aatcccctgt
     3481 aactgtaacg taattagcag tccaaggagc tcctgatgag tttattaaga ctggtgatag
     3541 accagttaat acatgtgttt ctatttcgcc tgtattaact gaattataat caacatcatg
     3601 tccatttaat aaatttatag tttgtgctac catttccata tggctaagct cttctgctgc
     3661 tatatcaaga aataaatctt ttatttgagg gtcttttatt ctaaaacttt gggacaagta
     3721 ttgcatagct gcttttaact ctccatttgc accacccaac tgttcttgca ttaaaacagc
     3781 atattgagga tttggtcttt ctacttttac ttcttttaat aatgctttat cgtgtttaaa
     3841 catatgaata cctccgtaaa ttattattca aaattattat ttacttaagt tgtaatatta
     3901 ttctatttta tttgtttatt gttgttattt tttacaagtg ttggtatttc aatgttttat
     3961 taaggtcagt tttgttgttt atgttttatt atgtttaatt taaatttatt ctattattta
     4021 ttttcggtgg caaaatggtg gcaagtttgg ttttgtcacc atttattttt atttgtaaaa
     4081 aatatttaaa aaatactgtt tttttcatat ttaaaattat atcttataac aataagggtc
     4141 tatattaaaa gactatgaaa ttgacttaat aattattgat aaaagaaata gtgtttttta
     4201 aataaatgaa aaaagcactc ccactaatgg aaaatgctct ctttaataat aat
//
LOCUS       DAKBNN010000268         4108 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000268 DAKBNN010000000
VERSION     DAKBNN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.274"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(302..520)
                     /locus_tag="OUB02_003601"
     CDS             complement(302..520)
                     /locus_tag="OUB02_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887322.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(521..928)
                     /locus_tag="OUB02_003602"
     CDS             complement(521..928)
                     /locus_tag="OUB02_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887323.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1014..1502)
                     /locus_tag="OUB02_003603"
     CDS             complement(1014..1502)
                     /locus_tag="OUB02_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HCT8887324.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(1707..2816)
                     /locus_tag="OUB02_003604"
     CDS             complement(1707..2816)
                     /locus_tag="OUB02_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCT8887325.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIAKGKREEKF
                     NIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3190..3360)
                     /locus_tag="OUB02_003605"
     CDS             complement(3190..3360)
                     /locus_tag="OUB02_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887326.1"
                     /translation="MDSNNNTIKSTVKKGISFDSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3532..3921)
                     /locus_tag="OUB02_003606"
     CDS             complement(3532..3921)
                     /locus_tag="OUB02_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887327.1"
                     /translation="MLADPCEEMIDKYLNKGTMYALYDNKELTCIAVVNEISKEICEL
                     KNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSESGVPFYEKFGFVYSHKIKN
                     FFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1387 a          675 c          367 g         1679 t
ORIGIN      
        1 tacctataat taattcattc tctaatctat tagcttggtt tttatttata agtcatttat
       61 tctctaatct attagcttgg tttttaccta taattaattc attctctaat ctattagctt
      121 ggtttttatt tataagtcat ttattctcta atctattagc tttctttttc atctataaat
      181 cattcattct ttaatccatt agcttggttt ttcatctata agtcatttat tctctaatct
      241 attagcttgg ttttttacct ataattaatt cattctctaa tgcataagta atacatgaaa
      301 tttaaatatt attttcttca ataaattcaa acaactcttc tacagttgta tcaaatacct
      361 tagcaatatc cattgccagt tttaaagaag gattgtagcg accattttct aaatgtacaa
      421 tagtttcacg tcttactcct actgactttg caagttcacc ttgtgtcata aggttctttt
      481 ctctatagga tttaattttt gtctttagta atactgacac ctaattgtat atcccctttc
      541 tgtcaaaata tataaataga attaacttca acaaggattg aataaacaaa atgactaata
      601 aagacattcc tatatttaaa tttgacacat ttatgtccaa agttacaaga aatatagata
      661 ctatagctac agaaaaaaac aatactttta ttgcaatatc attaactctt gatagtatac
      721 attttgatgc ctcatcaatt aaatcatcat actttttatt atagacccac tgagctccca
      781 tagctaatac ccaaataatt ataggaagat attcaatact acttccttta aaaaaatact
      841 tatttactaa tgtagctcca atacaataag aaattcccaa tatttctgaa ataagtgttg
      901 atttaatacc agtttgttgt ttagacatat aaaatccccc ttaaaaagtt atgtatttat
      961 aacacctctt gttataaata tataactttt ttgaataaat ttcaataaaa atattatttt
     1021 gcaacaaatt cttttattaa ttttacatta gatagaggca ctttagatgt cactccttgc
     1081 aatgtcaaat cttctatatt aacttcattt aattttgaac acagttctaa gtcaaatatt
     1141 ctttcccaac ttaccttctt ttcaatttgt tcatcttcat aaagctctac agcaatatcc
     1201 attaaaggta aatgccataa atcaaattct gataataaca cattgtcttc acttatttct
     1261 atttcaagta aaacaccttt ttttcctttt tctaaataac cactacatct taaatcaggt
     1321 ctcttcgtcc aaacccatat catactatca tcaaattcat atattctatt tgtcatttga
     1381 ctatacatcc atttgtatgg aatatcaaag tcttctaata taaaagaata atcatttgat
     1441 agatatttat tgtttttatt gttattccaa aattctaaag tctgtatagt ataaaatttc
     1501 atatataaat caaccccttt gtttttctat aagaaggaga tggttcaaac ttttattttg
     1561 aattatctcc tttatttata cacctctttg tacctattat ttatatatct ctttatatct
     1621 accatattta aataacaaag ttttatatta atttaaattt aaaagaggaa gctttgtatt
     1681 tatctagtgc catctcaatc atagtattag ttttgattat agactttttc aacctctgat
     1741 ttattcaaat catctataat atcgcatatt tcactcaaat tgtatccttt attttttaat
     1801 tttatgatga tattaaattt ctcttctctt ttcccttttg caataccttt ttcgatacct
     1861 ttttcaatac cttcttgaaa aatttcttct ttaaaatttc tacttttatt cataatatac
     1921 gcacctctct ttaaatttaa taaatagtcc tcattactac ctaattcttt taatctatca
     1981 agtgctcttt taataacata atccgtttta gatagtattc taatatctat atgagaagga
     2041 ttatcaataa gtttacccca tattagaact ctgtttgcta tacaactgga tttacgaatt
     2101 tttggaagtt ctataaaata taatgcagtt ttactagaaa aaatatctgg atgcccgtcc
     2161 attgttaatc tcaggcaatt tacataatct tctatgtgat tatattttac atgatttagt
     2221 atatgtattc caacagtttt tccaatttct ttatattctt taccttcttc taactttcta
     2281 ccatgtacac gactaagata atattcagct ctttccaaat aatcctcttc aagagctctt
     2341 tgcatctcaa tatttatatg gttacgttct tcatcttcag ccaaaatatc aagtctaggt
     2401 cttttctcac caatgtaatt aattgttggg tcggtttcaa ccttaattat tctttttatt
     2461 tgagtgaact gtttgatatt cctattagca tatggagcat ctttgaggat atcatttata
     2521 aaatttgcaa gtaaatcgta atcattattt aaagtaaaca atgatttaaa tacctcatca
     2581 tactcaggct tagcataccc atcttcagtt acaacttctc caacattttc actagtcaat
     2641 atttcttttg gaattaaatc cttgatttta tctaattttt cattagaaaa atcacctcta
     2701 ctaagtatgt catttaattt tttggtccat tctctgtcct tttcttcaat tatagttcgt
     2761 ttatactctt ttgatgtttt aataatctta gatttttctt ctaatttttc tttcatatca
     2821 acacctcttt tttattatta cccatttctt catcccaaag tctgaacttc taagtaaaat
     2881 ttgcattatt gaaataagaa ccttataaac ttatatatca ttactaaagt taagacttta
     2941 aagagaagca cttagattac aatagatttt agatagcttt ctttttaaaa ctcttgttga
     3001 taaatgaatg aatataagag tatgtattaa aatagagata ctcatctttt tcattgttta
     3061 agtaatgaaa atatatttta tttaatacaa aaaagagtgc cttataaact aaaaagttca
     3121 cttcgagaca ctcacttacg ttttgtttct taaacttcac aatattacat acttatttat
     3181 taatttaatc tatgcatact taacccaata ataaagtaca tatatccaac tcattaagcc
     3241 atgaaaaata gcccatggaa tagatttcca tgcagtataa gaaataatca ttgctaaaca
     3301 agaatcaaaa gaaataccct ttttaacagt tgattttata gtattattat ttgaatccat
     3361 aattaatcct ccaaaattca aaataaacaa gttattatat atcttatttg aatctaatac
     3421 aatataacag cttctaaata aattatactc ttattataca taatttataa attataattc
     3481 cgataattat atgaataaat tatgtatatt aacctaaaaa tattatccta tctaaaagtt
     3541 atatgataga tacaacatgt caacacattg taattctcct tcaaagattg gttctggata
     3601 attatctaca aaaaagtttt taattttatg tgaataaaca aatccaaact tctcataaaa
     3661 aggaactccg ctttcagatg taccaacaac cattgatgaa tattttttac tataaacatc
     3721 taaaagatga taaatcatct ttgaggcata acccatattt tgaaaatgct catatgtagc
     3781 aatgtttttt aattcacata tttctttgga aatttcattt acaactgcta tacaagtaag
     3841 ttctttatta tcatacaatg cgtacatagt tcctttattg agatatttat caatcatttc
     3901 ttcacatggg tcggctaaca ttaaaaggtc aatttgactt ttttatttat ttctatagtt
     3961 tttatttcta tcataagttc tccaccctca ctttattcaa accttaattt agcaaacttc
     4021 ttacagtact tatttagttc attttgatat atatttttac tttttaaaat atagtgataa
     4081 ttccctagaa tattaatact aataataa
//
LOCUS       DAKBNN010000269         4093 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000269 DAKBNN010000000
VERSION     DAKBNN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.275"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            262..1302
                     /locus_tag="OUB02_003607"
     CDS             262..1302
                     /locus_tag="OUB02_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8887328.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1658..2980
                     /locus_tag="OUB02_003608"
     CDS             1658..2980
                     /locus_tag="OUB02_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCT8887329.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(3167..4069)
                     /locus_tag="OUB02_003609"
     CDS             complement(3167..4069)
                     /locus_tag="OUB02_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT8887330.1"
                     /translation="MKKSTQKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDK
                     GFSYNTINNHKRSLKASFYIAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQAL
                     LSFIKTDNVYHKYYDDVLILLKTGLRISELCGLTIMDVDFIHEVVVIDHQLLKSKEQG
                     YYIETPKTKSGIRQVPLSRETIQAFQRVMKKHPKAEPFVIDGRSNFLFVNHKGKPKVA
                     IDYNALFVRMIKKYNKHHKDNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNI
                     SITMNWYAHASIDTAKSEVQRLIA"
BASE COUNT         1381 a          551 c          789 g         1372 t
ORIGIN      
        1 aaatttaata aggatttaaa ttaaaaattt taaaaattat ttccatataa aatatggcat
       61 tgtaattgct tatttaatag aggatttttg taaaatgaag aaagtatatc tatcaaatta
      121 aaactaagta gatatacttt ttattaagtt ttgttttaaa ataatattat taaattataa
      181 ctaagctttt ctgttatgat ataattaaat agataatgtg aatgattatt atatagaata
      241 cttatttgga gttgatattt tatggacaaa cacagtgatt cagtaatgta tggtggaatg
      301 gaagtatata atatttgggg aacaacaaca gtagactcca taattggaga gtctgatgta
      361 atgcgtgctc ttaagaatag aatttgtaaa attggaaata gtgattctac tgtagctata
      421 actggagaaa gtggcactgg aaaagaatta atagcaagag ccattcatct cgcaggaaac
      481 agagggaata aaacattcgt tgctataaac tgtgctgcta taccagaacc tttactagaa
      541 agtgaacttt ttggatatac aaaaggagct tttagtggag cagattcaaa aggtaaaatt
      601 ggtaagtttg aattggccaa tggaggggtt gtatttttag atgagatagg agatatgcca
      661 attcaactac aatcaaagtt attgagggta ttacaggaaa agaagtttac gagaataggt
      721 tcaaatgaac taattgatat agatgttaga atcatatcag ctactaatag agatttattt
      781 gagttggtta aggaaaataa atttagagaa gacttatact atagattgaa tgtaatacct
      841 ttagaagtgc ctccacttag agaaagagga gacgatatac aaatattaat aaacaatttt
      901 atagacaaat attcgagaaa gatgaagaaa aacgtgtacc atatagagcc agatgtggaa
      961 caaatgttgc ttaagtatcc ttggccagga aatattagag agttggaaaa tactatagaa
     1021 ctggcattaa accttcttga ggaagatgga ataatacata aaggaataat aaatagaaag
     1081 atacttgaat attttaagtt aaataatata aatataaagt tacttgagga aagtgactat
     1141 gagataagta tggaacaaga catacttaca cttgaagagt ttgaaagagc ctatataaag
     1201 aaagtgttaa agttttacgg aaatgatact aagtctaaaa aactggctgc caaaaaactt
     1261 ggaatatcac tagctacatt gtacagaaaa atagatgtat aataattagg ctttttattc
     1321 tcaattctaa aatttgaaat tataaaattg ataaaaaaca cttggaaaac tattttctaa
     1381 gtgttttttt gtgtaatagc attttattat ccaaaaataa aaaaaattta aaagaattta
     1441 aaaaaataaa tgctttaata aattatcaaa tactaacaaa tataataaaa attatatatg
     1501 taaaacaatt atgttaaaaa attggcatta taattgcttc atattaaatt gagatgacaa
     1561 aataaaaaaa gaaaggatgt tgtgtgagat aataataact gtcattaaaa aatagttggg
     1621 agtgataacc ttgagtaaat atcaaaagaa tgtttctatg tttgatattg atggaaggcc
     1681 accaatttta aagtgtttgc cactatcaat gcaacatata ctagcaatga tagttggaac
     1741 tgttacagta cctatagtcg taggaggagc tgtgggtgtt agccctacac aaataacact
     1801 tttagtgcaa tatgcattaa taatagctgg tatatcaaca cttatacaac tatatccaat
     1861 aggaaatatt ggttcaagat tgcctataat ttttggagtt ggttttactt atgtaccaac
     1921 acttgtagca gtcggaggaa attatggatt agcaagcatt ttgggtgcac aattaatagg
     1981 gggagtggct acagtattaa tagggatatt cataaaaaag ataagaaaat tttttccgaa
     2041 tatagttgca ggaacagtgg tttttacaat tggattatct ttgtatccta tagcaataaa
     2101 ttatatggca ggtggtgtaa atagtcctac ttatgcctct atgcaaaact gggcagtagc
     2161 ggctataaca ttagtggtag ttataggtct tagtcaattt gcaaaaggat atgcaaaatt
     2221 ggcagcaata tttttgggaa ttatagttgg atattgtgta tctttagttc tgggtataat
     2281 aaattttgac cctattagag aggcaacttg gtttgcgata cctaaaccgc ttgaatttgg
     2341 gatggaattt gatatgcctg taataatctc agtagtaatt gtaagtataa tcaatgctgt
     2401 tcaagcaatt ggagatttat cagctactac aatgggaggc atggatagga atatcacaga
     2461 taaggaatta tcaagtggag taataggaag tggaatttgt actatgatag gagctttgtt
     2521 tggaggttta ccaccatcat catatagcca aaacgtgggg atggtagcta tgaacaaagt
     2581 aataagtaga tttgtgcttg gaatagctgg tatatttatg ttattgtcgg ggtttgtgcc
     2641 taaatttggt gcattaatga ctactatacc atattctgtt ttaggaggag ctacaatatc
     2701 tatatttagt atgataacaa tgacaggtat aaagttaatt attacagaag agttgtcatc
     2761 aagaaatatt accattgttg gtttggcaat tgccttaggc atgggaataa catcagtacc
     2821 agcagcccaa gaggcatttc cttcatttgt aaagttagta tttggagaat ctccaatagt
     2881 aattgcaaca ttggttgcat tcgttttaaa tctaataata ccaaataaaa gtctagagga
     2941 tgaggctaaa gaaagagctg ttatggaaga gactaattaa gatataataa ttttaaataa
     3001 attatatatg tctttttgac tatcttttta tatcttctta aatgtccgca aagccttatt
     3061 ttataggctc tacgggcatt tttcttttgt ggtaaacctc acatatctag gtctatcttc
     3121 ttatattttc gctatcaagc gtggttaaaa tcgtggtaaa tacttcttat gcgattagac
     3181 gctgaacctc tgattttgcg gtatctatgg acgcatgagc ataccagttc attgtgatac
     3241 tgatatttga atgccccatg atatactgta aatctttcgg gttcatgttc ttgcttgcca
     3301 gccttgtgca gaatgtatgg cgtagcgtat gcggtgtgat atgtggcaag ggattgtcct
     3361 tgtggtgctt gttgtatttc tttatcatac ggacaaataa ggcgttgtaa tcaatcgcaa
     3421 ctttgggctt gcctttatga ttgacaaata ggaaattgct ccgtccgtct atcacaaatg
     3481 gttctgcctt tgggtgtttc ttcataaccc gttgaaatgc ctgtattgtt tctctgctta
     3541 atggcacttg ccttattccg ctctttgtct taggcgtttc aatataataa ccctgttcct
     3601 tgctctttag taactggtgg tcgataacca caacctcatg gataaaatca acgtccatga
     3661 ttgtcagtcc gcacagttcc gagatacgaa gtcctgtctt taacagtatc agcacatcat
     3721 cataatactt gtgatacaca ttgtccgtct tgatgaatga gagtagggct tgttcctgtt
     3781 cctctgtcaa tgcgactttc tctttggtat cattttctag gacttcactt aacttgaaat
     3841 caaaagggtt tttccttaca caatcgtctt gtatggcgat atagaatgac gcttttaacg
     3901 agcgtttatg gttgttaatg gtgttatagg aaaagccttt gtctttcatg cgtaatgccc
     3961 attccttagc gtcagagggt tttatcgtat caatgctcct agcacctaac ttgtcctctt
     4021 tcaataaccg catgagctgt tcccgttgtt tctgtgtgct tttcttcacg tttgccctct
     4081 gtgtgttctg ttt
//
LOCUS       DAKBNN010000270         3969 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000270 DAKBNN010000000
VERSION     DAKBNN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.276"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..619
                     /locus_tag="OUB02_003610"
     CDS             <1..619
                     /locus_tag="OUB02_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HCT8887331.1"
                     /translation="ELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDICLKL
                     NIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHE
                     LYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIH
                     IRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            573..1001
                     /gene="ytfJ"
                     /locus_tag="OUB02_003611"
     CDS             573..1001
                     /gene="ytfJ"
                     /locus_tag="OUB02_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HCT8887332.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1249..1689
                     /locus_tag="OUB02_003612"
     CDS             1249..1689
                     /locus_tag="OUB02_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCT8887333.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1705..3156
                     /locus_tag="OUB02_003613"
     CDS             1705..3156
                     /locus_tag="OUB02_003613"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCT8887334.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3247..3915)
                     /locus_tag="OUB02_003614"
     CDS             complement(3247..3915)
                     /locus_tag="OUB02_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HCT8887335.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSITAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1658 a          356 c          673 g         1282 t
ORIGIN      
        1 tgaattattt aattttaagt atttaataat agctgtaatt tttattataa gtgtaactat
       61 gttgatttta atatctcata taaacatctt aataatagct gatataaaaa acaaagatat
      121 ttgtttaaag cttaatataa aatacatgtt taatttaata aatataaata gacaacttta
      181 tccagctgaa aatagtaaaa ataatgataa aaaagagggg atgaagaata atatagattc
      241 aagtatatta ttagctgatg accttttgag catttataga ttattaaaaa agataaaaat
      301 acatgaactt tattcaaata ttaattttgg aactggaaat attggcttaa catcttctgt
      361 atatgtattg ataaatactt tgtatggaaa tttatttaat atgattgatg cagaaaaaat
      421 gtatttaaat gtaaatccag attttacaaa agattatgtt ttaggaaata taaggataca
      481 tataaggcca agaataaaag ctttatttaa tattataata atgattaata aaattatgaa
      541 taaaaataaa ggaaataagg aaggtgatag taatgagagc aacaggttcg atacagagtc
      601 ttatggaaac aactcttgaa acaattaaag gttctataga tgcaaatact attataggag
      661 accctataaa aacagatact acagtggttg tacctatatc aaaggttaca attggctttg
      721 gtataggagg aggagaatac tcaaaaggat atgatgataa agatagggaa attgaattaa
      781 aaaatgaaaa atcagatacg aattttgctg gaggtagcgc aggggctata tctgtacaac
      841 cagtagcatt tgtagtagtt gaaagtggag agactagaat aatgagttta gatagtaata
      901 tcaatctagt ggataatata ttatctataa ctcctagagt attggagaaa attcaaaata
      961 tatcacaaag taataagaat caagataaaa acaatatgta agaataaaat atttttaaat
     1021 atgtatatag ttttaagtaa agtagtgggg ttaatataaa tcccactatt ttgttgtgta
     1081 ctttaaacat ttatatctaa taagatttta gtttaaaaca atttaaatag ataataaagt
     1141 tgatatatag tataattata aaaagaaaag aagtattata ttatagatat ataaacgcat
     1201 ttgagatata agagggagtt ataaatcatt agttttgagg tgaatgttat gagcgataaa
     1261 gaattatcta ttttgttgtt agaaagtatg aataattttc ataatttaat aaagattatt
     1321 aataacgaaa gatataaaaa agataaggtt cttacagaaa gacagttctt tgccttagta
     1381 aaaataagaa aacatgataa aatagagctt aaaaatctaa gtagagattt gcatgtatct
     1441 acttctagtc tgtgtatact acttaataaa ttggttgagc aagagtatgt atatagggaa
     1501 gaagatagta gagatagacg aaatactttt tatggaataa ctaaaaatgg agaaaagata
     1561 ttggataatg aaatactaaa gtttgtatct ataataagtg ataaaatgga ttgcctagat
     1621 atagataata aagataaatt atttacttct ttggaagagt ctaaaaatat aatagaacaa
     1681 ttattttaaa aaggagaatt tattatgaat ataaagtatt ctaaagagat attaaaaagt
     1741 ttagaaaatg ctataacagt agtagctcta tttgagggag aagatgtttt tacgacattt
     1801 gataaaataa atatgataag cagaaatttt aattctaaag aaaatagtat agatactgta
     1861 acattaattg aaaataacaa tttggtaaat gtttgttttg ttggttttgg taagagagag
     1921 ttattgaata gagaaaaaat tagaatcata ggtgggaatt tgtgtaaaaa attaaaaaat
     1981 atattgaaaa atgaagcata taagagtagt gatgttaatg tactgaatct taatttaaat
     2041 agtgatgaaa taggttgttt tgtagaaggg atattacttg gaaattataa gtttgataag
     2101 tataagacaa aaagtaagga ttttgaaaga aaagaagttg atactataac tatatttact
     2161 gaaaaagaag ttgagtgtca aatagaaaag gctaaaatat tagctaattc aactataata
     2221 gctagaaatc tagttaatga accatcaaac gtaatatatc caaaaacatt agcagtagaa
     2281 acagttaaac ttggtgcaga gtttgggttt aatgttgatg tttatgaaga agaaaaaata
     2341 aaggcattgg gaatggatgc ttttttcgct gtttcaagag gttcaattaa taaaccaaga
     2401 ttcatagtaa tgagatactt tggagataaa gaaagtacag atatattagg tctagtaggt
     2461 aaaggattga cttatgatac tggtggatat tctttaaaat caaatgcttc tatgctagat
     2521 atgaagacag atatggctgg tgctgcgagt gttataggtg ctatgtgtgc tatatctcaa
     2581 agtaaattaa agaaaaatgt tatagcagta gtggctgcat gtgagaatgc tttatcagga
     2641 ggttcataca aacctggaga tataattagt tctatggcaa agaaaactat tgaagtttta
     2701 aatacggatg cagaaggtag acttacatta gctgatgcaa tatactacat tataaacaat
     2761 gaaaaagtaa caaaggtggt tgatgtagct acattaactg gtgctgcttt aacattgtta
     2821 ggtaatgttg caactcctat agtaacaaat aatgatgatt tttattgtga attagaaaaa
     2881 gctgccactt tatcagggga aagggtatgg aagatgccaa tttatgatga atttaaagat
     2941 atgataaaag gagaagaagc agaccttaaa aacactggtg gtaaaaatgc tggatgtata
     3001 acagcaggag cttttattgg tgaatttgtt gggaatactc cttggataca tatggatata
     3061 gcggggacaa gtacatcatc aaaatcaatt ggatacaaag caaagggggc tacaggagaa
     3121 cctgttagaa cattgtacta tttggcagaa atctaaaaaa caattataaa atataattta
     3181 taaagccacg tggatatttt tgtaatttaa gattatctat gtggctttat tttttattta
     3241 agtaaattat ttactctttt tctttttttc aagtataaaa gtatctctat taaataaaag
     3301 agcaggtatg tacataatac tagtaacaaa aataaacgta aaaatagcag ttatgctatg
     3361 acttaaaaaa ccataatagt acctagagtt ggtaaatgtg tttagtaaaa atatgaatga
     3421 aaaataaata aaacatatag ctaagttatc tctaagtgca tatctgttaa ctctaaattt
     3481 tctaacatca accatatata tatctgcata aattatcaag cacttagaga agataaagat
     3541 aaaatatatt aagctaggaa attcatagcc caagttttta gaaaaaatga gttctccaac
     3601 tgaacaaact aaactccaag aaaaaaatat attaaaaaga tgatactgct taaaaattag
     3661 tatggctata cacagatata tacaaatata attgattcca attggtaaag agtttagagt
     3721 actataatta cctgaagaaa taagtaataa ttgctctaga gccataatta ttacaagtaa
     3781 tgtacaaatt atcttctcaa ctgtatagct ataaggtaaa atatttttgg ttagttttgg
     3841 acaatagtat aaaaataatc caaatataaa gagacttaca aattgttcac tagaaaaaag
     3901 aaaaatttta aacatataac attctctcct ataataaatt taatatgtat ctatataaat
     3961 atattcatt
//
LOCUS       DAKBNN010000271         3941 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000271 DAKBNN010000000
VERSION     DAKBNN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.277"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(325..1575)
                     /locus_tag="OUB02_003615"
     CDS             complement(325..1575)
                     /locus_tag="OUB02_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HCT8887336.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRMIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1607..2548)
                     /locus_tag="OUB02_003616"
     CDS             complement(1607..2548)
                     /locus_tag="OUB02_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCT8887337.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2788..3864)
                     /locus_tag="OUB02_003617"
     CDS             complement(2788..3864)
                     /locus_tag="OUB02_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCT8887338.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1197 a          716 c          415 g         1613 t
ORIGIN      
        1 taataattat atttataaaa ataaaatata aatttcaaaa tcactttttt tattaacttt
       61 tttataatta taataaaaat atataacagt actattataa atacacattt aaaaccttat
      121 aaatatacaa atacaattat atgaattttc atcctcaatt atagcataga atatgactat
      181 ctatatccta aaattatcca tttctaaaaa aaaggtaact attattaaga atatcaactt
      241 atagaattta tattccacat acgattttca atcaaaaaaa caagggctaa aaagtgaatt
      301 gtgcactttt tagcccttta ttaattattc atttatttca tgtaagaata gaccacatct
      361 tactttaggt tcaaaccatg ttgacttagc tggcattatc ttatttgcat ctgctactct
      421 tattaaatct tcaatactag taggatacat tgcaaatgct actttcatat cttcattaac
      481 tcttttttct aattcttcaa gacctctaat tccaccgata aaatcaattc ttttatctac
      541 tctaggatta tcaattccta aaatagcatc aataacatag tcttgtaata tagaaatgtc
      601 taatgaatca acagggtctt ctatttctaa taaattttta tttgctttca ttttatacca
      661 cttatcacct aggtacattc cgaaagttcc ttttttgtca ggtttatatt tttcttttcc
      721 ttctatttct tctagttcaa aattttcttt tattttattt ataaattctt cctcgcttaa
      781 accatttaag tctttgataa ccctattata atccaaaacc attaaattct catctggtgc
      841 aatcattgct atgaagtaat taaattcttc ttttccagta taatttggat ttttagctct
      901 catttctttg gctacatttt ctgctgaagc tgtcctatga tgaccatctg caatgtatag
      961 ataatctaat ttttcaaatt tatctactag gttatctatt atatcatcat tatcaatctt
     1021 ccatactgta tgctctatct tgtcatctgt tatgaaatca tataaaggtt cattattatc
     1081 cataaaatca ttgattatat catctatcat cctttgatgt ttgtaagtta caagtatagt
     1141 tcccgtatta gcatcacagt attttatgtg tttagttcta tcaatttcct tttctggtcg
     1201 agtgtactca tgttttttta ttgttccatt caagctttca tcaactgata tacatgctac
     1261 tataccaact tgagctctac catccatgat ttgtttatat atatatatac actctttgtc
     1321 atcttttact agaatttctt gttttcgaaa ctcatctaaa ttttctcgcg cttttaaata
     1381 aactttttca tcatataaat ctatattttc atctaaatca atttctgatt tatctatatg
     1441 caaaaatgaa tatggattcc cttctgcaat tcttctagct tctttactat tcataacatc
     1501 gtaaggaagt gctgctactt tatctactat atatttattt ggtctaatcg ccttaaatgg
     1561 tttaatcgtt gccattacta atttcctcct aattatacaa aatattttat aatgctaccc
     1621 ctattagatt gttgaagtca agaaagaaat cttgaatcac atttactatt tcgtctccta
     1681 ttctttcttg agcttctact gtagatgccc caatatgagg tgtaggagaa actcttggat
     1741 gatttactaa gtctaaattt aatgtaggtt cttgttcata aacatctata gcagctgctg
     1801 ataatttacc ttcatctaat gcttttagta aatctttttc atcaactaaa ccgcctcttg
     1861 cacagtttat taaataagcc cctttcttca tcttatttat ttctttttca gttattactg
     1921 cccccttatt cttatcaaat ggtatgtgta aagataagaa atctgcattt tctaaaactt
     1981 cttccatatc acagaattta taattgttaa agccttgcgc ctcacccatt atgtcagtat
     2041 atataacatt catacctaat aattctgctc tctttgcaac ttcttttgca attcttccaa
     2101 aacctataag tccaagtgtc tttccattaa tttctgtacc tttatacttt ttcttctccc
     2161 attttccatc tctcatagtt acatttgcag tatttatgaa tcttgcaaga acaaataatt
     2221 gacctattgt aagttctgca acagatatac tgcttgcatt tggagtattc ataactttta
     2281 tgccattagc ttgagcatat ttaacatcta tattatccag acctactcca cctcttacta
     2341 tcaacttcaa ctttttaccc tctaatgctt tatctattac atctttagta actttagtag
     2401 ctgaacgtac tacaagtaca tcaacatctt taagtttatc tcctaaaaca tctttttcat
     2461 aaaactgctc tatcacatta aaatctaact ctctcaattt tctcgctgct tctttctcta
     2521 ttccatcagt aactaatata ttatacataa caaactcctc caatttttat tcacttaatt
     2581 tttccaattt ttatttttta aagtattttc atcaataaaa aactttactt aattcaactt
     2641 tctgtttata taattttcaa aatatttttt tattaaagtc atatcaaatt tttctattgt
     2701 ttgttaaaaa tttaatatgt aatttttata aaatattatt tattatatat tctaaattta
     2761 tcttaaaaca tacatatttt tacaatatta tttattaaaa cctaatatat catcaatatt
     2821 atctaataat tcttgtacat cttcgacagt atagtcaccc atatgtgaaa ttctaaaagt
     2881 tttttctttt aaatctccat agccatttgc tatttggaat cctcgttctt gtaattttga
     2941 atttaaattt gatacactaa taccttgagt attttctata acagtaagtg tattggataa
     3001 gtgattttca ttggtaaaaa tttggaagtg ttttttagcc cagtttctaa ctaagtttgc
     3061 catgtcttca tgtctgttga atctgttatc taggccttca tctaatatat tatctaattg
     3121 gaaatctaaa gcgaacatgt gagaaagaga tggtgttgaa gggtattggt agtttttctt
     3181 gattaagtat tcatacattg ctaacaaatc aagatatact cctctaaaag gtacttgctt
     3241 agctctatcg attgcttttt gagaaaatgt gcaaatagac attcctggtg gtaatcccag
     3301 tgctttttgt actgatgtta tacaaatatc tattccaatc ttatcaacct cgacttttat
     3361 tccaccggct gaacttacag tatctacaca gtagattaca tcttcatact ttttaacaac
     3421 ttctccaatt tcttctattg gatttcttat tccagttgaa gtttcattgt gagttacagt
     3481 tattaaatcg tattctccgg tagaaagcac cttatcaacc atttctggcg ttgttgcctc
     3541 tcctaattca actttaaata tatccgctgg gacattatta gccactgcca ttttgtacca
     3601 tctatcacca aaggatccac aggaaaatac tgctgccttt ttggatgtac aggaacgaat
     3661 ggagccttcc attaaaccag ttccggatga cgtggataaa agtatcgtgt tgtttgtata
     3721 gaatagtttt tgcatctttt cacttatact cttttggagc atagatgcat cttttcctct
     3781 atgtcctatc attggtgtag ccatcttttg caagacttct tctttaacat caataggacc
     3841 aggaataaat aatttcttac tcattataat tccccccttt ttagtttgta atatggatag
     3901 cgtttttact gtccttattt ttaataacta taatatcaag c
//
LOCUS       DAKBNN010000272         3935 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000272 DAKBNN010000000
VERSION     DAKBNN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.278"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            52..519
                     /locus_tag="OUB02_003618"
     CDS             52..519
                     /locus_tag="OUB02_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887339.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            562..2325
                     /locus_tag="OUB02_003619"
     CDS             562..2325
                     /locus_tag="OUB02_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HCT8887340.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            2333..2941
                     /locus_tag="OUB02_003620"
     CDS             2333..2941
                     /locus_tag="OUB02_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCT8887341.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            3013..3582
                     /locus_tag="OUB02_003621"
     CDS             3013..3582
                     /locus_tag="OUB02_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCT8887342.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         1615 a          346 c          675 g         1299 t
ORIGIN      
        1 aaaatattat aattgtaaaa cttacaaata tatgtaaaat atttaggagt gatgaaaatg
       61 ttaaatggga taataaagct tttttattct gaaattgtaa aaacaaagat taataactta
      121 gattcaagtg gatattcttt aagagatgaa tgtaaagaaa tcagaaatat aactctaaag
      181 atagatagtg tattttttaa gcttttaaag ttagcttttg gttcaatatt gcttatggta
      241 gcttttaatt taaacatggt tttaggtata ggagtgtttt tagttggtgt agtttatata
      301 ctatataaaa gaaatatgga agaacaagta aaagagcata taaataatgt gaaaaataat
      361 atcgacatct ctaaaataac ttttatggat gaaaaaggga gaacaggtct aaatgcatta
      421 attacattac ttcttatagg attattgaca ggatttaact ggatattagt tgctagtttt
      481 gtcgttgttt ttgtttttac tataaagaat gtatgttaaa atactaataa atagtttaat
      541 atataggggg agaaattgag tatgactaat ggcagttata aaatgaagaa tttagtggaa
      601 atatccgcta tggtgactga atctacagat ttttttaata taaaagatga cataatagaa
      661 aaaatgttag aagttgttca ccctacaaag gcatgtgtga atctatttta taaaaatgat
      721 tctaaatatg cctacttagt atgttcgcaa accttagaat atgtacctca aatttttcca
      781 ataaatagtc ttaaaggtgc aaaaattgat tttgacacat atccagagta tatacatgag
      841 gctgtaaatg aaaaaaagat aatatatgtg gaaaatgtat ttgaagattc tagagcagaa
      901 ggtgaacgag atttagcaaa agctgaaggt tatattggga gaatagtttt tccttttata
      961 ataaataacg tagttgttgg atttatgact tgttttttaa aagaggatga ttatataaca
     1021 gaacaagata tagattttat atcatctgtt gcatcactct taagtttatc aatagaaata
     1081 actaataaaa ataataatgt acaaatttta attgataaac taagaggctc aataagtgcc
     1141 ataaatgaag ctactaaaaa actatacctt aataagagta ttgatggatt tttggaaaat
     1201 ttaagtaaac aagcgtgtaa tattactaat agtaaagaag ctttaataat tataaatagt
     1261 catcataata aggatgctat agttagttgt tataatgttg agcaaaaaaa gaaaacaaat
     1321 ttatatccta tgattgatat gtttttaaag aaagagtcat tgggggggta tttaaataat
     1381 ttaaaaccat gctcaaaaaa gggaataaat ttggaaagtt atatatatta caaacttcag
     1441 gataaaaatg atactatagg atgtatagtt tgtgcaaata gcaaaaacta tactagtgat
     1501 gatttaaata ttcttagtat attgtccaaa caagtttctg ttggtatgca attatatgaa
     1561 cataatgaaa aggaagtaaa acacaaagtt cttgaaaatg agttgaacat attaaataaa
     1621 caacaacagc ttattatgga taaaggtaaa atggattgta atgatgagaa agaactttat
     1681 ttttaccata aaccagcaag ggtagtaggt ggagattttt attatgctac aaaagtggat
     1741 gacgaacata tagtttatat tgttgctgat gtaatgggac atggaatggt atcaaattat
     1801 gttgttgcaa tgataaaagg tgcttttaaa gtattatgca atcaatacaa tacaccaaga
     1861 gaaataatga caaatttaaa taggatgtta tatgatgaat ttgacaagat gggagtattt
     1921 acaacttgtt tgataggtat tatagatact aaagaaaata tgattactgt gtctaatgca
     1981 ggtcactata gtcctgtagt tgtaaagaaa gatggcacaa tagaaaataa tttaaattgt
     2041 aaaaagggca tacctattgg aataatggaa gatgctactt atgaaaataa tactctttca
     2101 atggaagatt atgcaatggt atgtatgtac actgatggaa ttttagagat aaaaaattca
     2161 tcaaaagaag agtatggtat aaaccgatta gaaaactttc taaaagaaaa ttttagatta
     2221 aatcaacagt taatagtaga aaatctaaaa ttggatttaa agaacttctc atcaaaagat
     2281 aattatgatg atgatatttt aatagttatg ttaaaggata ggtagattga ttatggattc
     2341 aaacgataaa aaaggactta aaagtccaga gattataata ataaatggaa gtccaagaat
     2401 taataaaaat tgcagtttta tagcaaatga agtaaaagct atgctagaag agtgtggatt
     2461 aagttgtaaa atatttaata tctacaatat ggatattgaa tattgcacag catgtggatt
     2521 ttgtgaaaaa acagggtatt gcaagtttaa tgatgatatg aagccattat ataaaatgtt
     2581 tgatgattca aaaggaacta tagtaataag tcctgtacat tttgattcaa tatcagctaa
     2641 attaaagact gttgttgata gaactcaagc tatatatgca agtaaatatg tattaaataa
     2701 accttcaatt gatagaagta agaaacggat aggaatgtat attgcagtgg gagggagtaa
     2761 atcatatgat tcacagttta aaggtggaca gattgttatg gattttttct ttaagagtat
     2821 aaatactaaa cttttacaca atatatattt aaacaataca gatgatgtat cttatataga
     2881 taatcaagaa tttaaagata taatatataa aaatttaaag atatatattg attcaatata
     2941 atttgatttt gtaggtaata ttatgtataa ttgtattgca ataatttaaa taattaaaag
     3001 gagggaattt caatgaaatt accaatagca ttatctaata aacatgttca tttgagtcaa
     3061 gctgatatag acgtattatt tggagcaggt catgagttaa ctaaatttaa accattatct
     3121 caaccagggc aatatgcctg tgaagaaaaa gtggatttag taggaccaaa aggaacttta
     3181 aaaggagtta gagtactagg accagctaga gctcatactc aagttgaaat atctttagca
     3241 gatggtttcc cattaggagt aaaagcacca ataaaagaat ctggtgatat agctggaact
     3301 ccaggtgtta aattagtagg acctgctgga gaagttgaaa tgaaagaagg agttatagtt
     3361 gcttctagac atatacatat gagcaatgaa gatgctgcta aatttggtgt aactgataag
     3421 caaatagtaa aagttaaaac atctggacca agagcattag tatttgaaaa tgtattagta
     3481 agagctagtg acagctttaa tctagaaatg catgttgata tggaagaagg aaatgcagca
     3541 ggcgttaaaa atggagattt agtagaatta atagttgatt aatattataa atagacttta
     3601 tgctagtaga tattttattt gctagcatat ttttaaatgt aataaatttc tataggataa
     3661 atttaatcga ttagattaat ttataaaata aaggggtatt ataaaaaatg taataaaaac
     3721 ttacaaaaat atgatgtaat atggtaaaat tatatatata aaaatttaca tataataaag
     3781 ataattattt tactttttgt actaatataa acaaatagat aaaaaagttt gaaaagtcta
     3841 acttaattta ttatatattg tatacaaaat tatgaaaaaa acttaacata tttaatttta
     3901 aaaaaaatct acttaaatat gtaattaatt tacaa
//
LOCUS       DAKBNN010000273         3847 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000273 DAKBNN010000000
VERSION     DAKBNN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.279"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            85..489
                     /locus_tag="OUB02_003622"
     CDS             85..489
                     /locus_tag="OUB02_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HCT8887343.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     KDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIVYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            506..1102
                     /locus_tag="OUB02_003623"
     CDS             506..1102
                     /locus_tag="OUB02_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887344.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            1139..3733
                     /gene="clpB"
                     /locus_tag="OUB02_003624"
     CDS             1139..3733
                     /gene="clpB"
                     /locus_tag="OUB02_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCT8887345.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
BASE COUNT         1617 a          370 c          697 g         1163 t
ORIGIN      
        1 atttttagtt ttgacataca tatttttttg taatatattt atattatcaa ttagagataa
       61 ggagagtgag aagagatatt taatttggat aagacattaa aaaaggattt aaatcaggaa
      121 tatacagaga gcaaaagttt agttatgaat aaaagtaagt atatgaatgt ttgttttaag
      181 aattatatta aagtagtaga aggaatgtat actaagaagg atttaattcc attaaaatta
      241 ataaactcta tggataaaaa taataagatt agaaaaacat tatatgaatt atcttgtgaa
      301 tttgaatatc caaaaacttc tttatcttca ttgtttacgg agctgaaaaa aatggatttt
      361 ttacaaagag tgaaaaatgg tgagtatatg ataaatccac atatagtata taaaggtagt
      421 agagctgata aagaaagttt attagcagaa tataatcaga taaaaagacc taataaggtt
      481 agtaaataag gaagaaggtt tttatatgag tttaaaactt gatagaaatg ttctacagtg
      541 gtttgactat gtatttgaga atgaaaaaac tagtttgaga cactataatt ttaattgtac
      601 actaaaggag attagttcta ccagtttgaa caaagtagct tttattttag aaaaaaataa
      661 ctctaagtac tggaagcttt attttgaaat accagctgaa gttactttaa aacttaagca
      721 aaacattcat cctttattta gggaatatat atacgaacaa atatcactgt ataacaataa
      781 tcaaatttac aattttgtaa attcaaacat attaaaagtg tttaataata ttgctattta
      841 tcaatataat attttagaaa atctatacac tattgatttt aaaaagagtt ttatagataa
      901 atgtcagtat ttattaatag gtgaaaaaag acttatagat gaagacttgt atttaatagc
      961 aaaatcaaaa gaagtattcg atttttttaa ttcagatgga acatttaatt taacattgag
     1021 tttcgatata caaaaaaacg agaatttact tgatagtctt ttagagctta gaaaatcaat
     1081 aattataaat gagagaattt aatttatttg gatataatat taaaatggag ggatttatat
     1141 ggacgtagaa aaaatgactt taagagttca aaaaagttta aatgaggctt ataacgaagc
     1201 agtaaaaaat cataaccaac aagtagatgt aattcatctt ttctcagcac ttattaatca
     1261 agaggatgga ttgataccaa atataatcga aaaaatgaat atatcaattg attctttgag
     1321 aaatactgta aactttgaaa ttgataagct tccaaaagta tatggagaag gtgctgactc
     1381 acaaggagta tcagctacta gaaaaataaa tgaggtgctt ataaaggcag agagtatttc
     1441 taaagaattt aaagattcat atataagtgt agagcatgtt atgcttgcaa tgatggaaac
     1501 agaatcaaaa tcagctgttg gtaaaatatt aaaacaatat aatataaata agaatgactt
     1561 tttaaatatt ttatcacaag tgagaggtag tcaaagagta gaaacacaag acccagaagg
     1621 cacatatgat gcacttgcta gatatggaac aaatttagta gatttagcca agaaaaataa
     1681 attagaccct gtaattggga gagatgaaga aataagaagg attataagaa ttttatcaag
     1741 aagaacaaaa aataatcctg tattgattgg tgagccaggt gtaggtaaaa ctgcaattgt
     1801 agaagggtta gcagagagaa tagtaagagg agatgtccca gaaggtttaa aagataagat
     1861 tgtattctca cttgatatgg gagctttaat tgctggtgca aaatatagag gtgaatttga
     1921 agaaagatta aaggcagttt taaaagaagt tcaaagttct gatggaaaaa ttatactgtt
     1981 tatagatgag attcatacta ttgttggagc tggtaaaaca gaaggttcaa tggatgcagg
     2041 taatcttatt aaacctatgc ttgcaagagg tgaattgaac tgtattggtg caactacttt
     2101 tgatgaatat agaaagtata tagaaaaaga taaggctctt gaaagacgtt ttcagcctgt
     2161 aattgcagaa gaacctactg ttgaagatac aatatctata cttagaggtt taaaagaaag
     2221 atttgagata catcatggag taagaataca tgataatgct attgtagcag cagcaaaact
     2281 ttctcataga tatatacaag atagattttt accagataaa gcaattgatt taattgatga
     2341 agctggagct atgattcgta gtgaaataga ttcattgcca acagaattag atgttgttag
     2401 aagaaagtta tttacattag aaactgaaag agaagcatta cttaaagaaa atgatgataa
     2461 aagtaaatca agattggatg atattcaaaa agaaatagca gagttaaaat ctaaaaatga
     2521 tgaaatgaca gcaaaatatg aaaaagaaaa gagtcaaata ttagatatta aaaacttaaa
     2581 ggctcaatta gacgaagcta aaggtaaagc tgaaaagtat gaaagagaat atgatttcaa
     2641 caaagcagca gaggttaaat atggtgaaat acctaagtta gaagaacaaa taaagcagta
     2701 cgaagaaaat atgaatgatg gttctgaaaa ttctctttta aaagaagaag ttactgaaga
     2761 agaaatatct agcattgtat caaaatggac aggtatacca gtaactaaac tagttgaagg
     2821 agaaagagaa aaattactta aactagaaga tgaacttcat aaaagagtta ttggacaaga
     2881 tgaagcagtt acagcagttt caaatgcagt aatacgtgca agagcaggat taaaagatga
     2941 aagaaaacca attggttcat ttattttctt aggccctaca ggtgttggta aaactgaact
     3001 tgcaaaaact cttgctagaa atttattcga tagtgaagat aatataataa gaatagatat
     3061 gtctgaatac atggaaaaac atgctgtatc aagactagtt ggacctcctc cagggtacgt
     3121 gggatatgaa gagggtggtc aacttacaga agcagttaga cgtgctcctt attctgtaat
     3181 actatttgat gagatagaaa aagcacatga ggatgtattt aacatgttct tacagatatt
     3241 agatgatggt agacttactg ataataaagg taagacagta gactttaaaa atactcttat
     3301 aataatgact tctaatatag gaagtaatta tctattagaa gcaggaggaa atataacaga
     3361 gactactaac aatttagtaa tgaatgaaat gaagcataga tttaaacctg agtttttaaa
     3421 cagagtagat gatattataa tgtttaagcc tttagaccaa gaaaatatca agaaaattat
     3481 agatatattt atgaaagatt taaaaaatag attaaaagaa aaagatatta ctattgaagt
     3541 tacaaattca gcaaaagatg ttatggtgag agaaggatat gaccctgtat atggagcaag
     3601 acctcttaag agatatatag gaaatacatt agaaactata attgcaaaga aacttattgc
     3661 tggagatgtg tataatggat gtactatagt tatagatgga aaagatgaaa atattgaagt
     3721 tttagttaaa taataaagga atcagttgag cataatatac taaatatttt gctcagctga
     3781 tttttattta taaactatag gaaaagaaat attttatcaa attaataaaa catttttaag
     3841 tcaatat
//
LOCUS       DAKBNN010000274         3841 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000274 DAKBNN010000000
VERSION     DAKBNN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.280"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(79..1500)
                     /locus_tag="OUB02_003625"
     CDS             complement(79..1500)
                     /locus_tag="OUB02_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HCT8887346.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(1646..2416)
                     /locus_tag="OUB02_003626"
     CDS             complement(1646..2416)
                     /locus_tag="OUB02_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887347.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(2421..3623)
                     /locus_tag="OUB02_003627"
     CDS             complement(2421..3623)
                     /locus_tag="OUB02_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCT8887348.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         1277 a          704 c          398 g         1462 t
ORIGIN      
        1 cttatctcta ttttgagata gcctcttttt tgaacataat ttaaatacaa ttttttgatt
       61 tttatagtta ttattttact aacttgccaa tttatgatta ttttgttcct taatatgctt
      121 tgctttttta aagaataaaa gaccaagaat tattgctaca ataacaccta ttccattaga
      181 tatattaact ggtagtctaa atccttcttt tgcttgcatt atataactaa atgttactac
      241 agtcataaaa agtgctggta atgtagttat ataatggttt ttatttgctt tgactaaata
      301 tgcagaacca gtccacaaca ttatcattgc aagtgtttga tttgcccaag agaaatatct
      361 ccaaattata ttaaaatcta taaatgtaag agcaacacca actgcgaata atggtaaagc
      421 tattaaaaat ctatttttga ctgcatcttg attataattc attgcatctg ctattgtcag
      481 cctagcactt ctaaatgctg tatctccaga agttatagga catgccacaa ctccaagtat
      541 agctaaagct cctccaatag gacccattaa tccaacagat actgtattta ctactgttgc
      601 tgctccacca tcattaaata tcacattaag ttgaggtatg ccatggaaat atgacatggc
      661 tgctgctgcc caaactaaag ctattatacc ttctgcaacc attgacccat aaaatactgg
      721 tcttgcctca ctctctttct caacacatct cgccataagt ggtgattgag ttgcatgaaa
      781 acctgatatt gctccacatg ctattgttat aaataagtat gggaataaag gtgttccatt
      841 tggattcata ttttgtaaat ttatatttgg tatgttatac ccctttatta tcaatcctcc
      901 agctacacct actgccataa ttaataatgc tgctccaaat aaaggatata ttttaccaat
      961 caatttatct attggtaaaa ccgtagccat aatataataa agtattatca ctccaaccca
     1021 tatttgtgcg tttacacctg tcaatgtctt taataatcct gctggacctg ttacaaatac
     1081 aactccaacc aatactaaaa gaataacact aaatattctc attgctattt ttgcaccatt
     1141 tcctagataa agtcctacca actctgatgc agatgtacct ttgtttttca ttgatagata
     1201 tcctgaagca aaatcgtgta ctccaccagc aaagattgtt ccaaacacaa tccataaaaa
     1261 tgctgatggt ccaaataatg ctccttgtat agctccaaat atcggaccta accctgctat
     1321 atttagaaac tgaattaaaa agattctctt ccatttcatt ggcacataat ctactccatc
     1381 cctacaagct atagctggag tttgattttt gtcattggtt ccaaaaacct tttctacaaa
     1441 agtaccatag acaaaataac ctactataag tactactatt gaacctaaaa agcttaccat
     1501 aaattcatcc ccttagtttt tatataattg ctttataaat ttttatgagt ttaagaaaac
     1561 tatttccagt tcaaatagta atactttttt atgctaatat aaatattaat tacattaagt
     1621 gcatatttaa gtacatgaaa tgactttata tatgcattaa cagcctaaat tcttttactt
     1681 tgcttctact tacagttact tcataatcac tattttttaa ttttaatatg tatgtattat
     1741 taaaccatgg tataatttct tttactttgg ttaagtttac gatataagac ctatgtgttc
     1801 taaaaaatgt tttatcattt ataagatttt cgacttctga tatgccttct tttaatataa
     1861 acttttcttt ctcagtatat ataaatgtct gcctttcatt agcttcacag taatatatat
     1921 catcaatatc tattacaact aatttatcac ctttccaaag gctaatttta tgtgttattt
     1981 cttctatctc ctgatttact gcttccttct tatatttgta taaattacta tttacattag
     2041 atagttcaat atcactcatc tcactttttt ctaatttatt cagcatagaa ataattctct
     2101 cttcagaata tggtttcaat atataatcaa atacatccaa agaaaaagca tctactgcat
     2161 aactttcata tgctgttata aaaacaattt taggtcttga tttaaactga tttaatgttt
     2221 ttgcaagtag cattccatca agatgaggta tatttatatc taaaaaaatt acatctattt
     2281 tattttcctg aataaagtct aacacctcta taccattggt aaattcactt actacctcga
     2341 ttccactttt attttcaata aaatatctca attcctttct tgctggaaat tcatcttcca
     2401 ctattatagc tctcatactt ctactccttt acataaaata ctatcaaagt cccattatct
     2461 attcttctta tattaagccc tcttccatac atcagtttaa gccttaaatg tacattataa
     2521 agcccaatct tattttctgg catattatct tgatatacat tatttataat ttcctcactt
     2581 atacctatac cattatcctc tattgatatt ctcacagtat tttctaatga atacggtaac
     2641 ttttttatgg atattttcac tacaccagct ctaccacttt ctaaaatacc atgtattatt
     2701 gcattttcca ctaatggttg tataatcaaa cttggaatct ttatatttac atcttctata
     2761 tcataagata cagttagtaa ttctccaaac ctagcttttt ctatctctac aaaagattta
     2821 acttgttcaa tctctttatt tatatcaatt aagttatcac tatattcaag attatatctt
     2881 agataagttg ataaatttat tattaactct ctagccttat ctggactaaa tcttataaat
     2941 gaagctattg tgtttaggga attaaacaaa aaatgtggat taatttgtct ttgaagtgct
     3001 tttatttctg ctttatttct cgcttctata tcttttttat ctttctcaat cccctctact
     3061 atagatataa caaatcctat tccaatctgc ccaactatta aaggtaaata tattccaccg
     3121 attatttgaa ttgctaaaga atgtggatat gatattaagt atataagtaa tatagtaaaa
     3181 ctttcagaaa tcatgcctac caatattcca tacattactc tatgttgttt tggtatcctc
     3241 ttatatagaa atcctgataa tacacctgct agtatacttg ataaaagaca tggaacagat
     3301 gttatccccc caatatccat aaaatatcta tgaatccctg aaaaaactcc tgctgtaata
     3361 cctaccatcg gaccaaataa aatgccacct gatactatag atattattct agtgtttact
     3421 attgacccca tataatttat accactataa gttccaaata tggctaaaaa agtaaaaact
     3481 aatgctatac agaccaagtc attaaaacta tattcttcct tttgaaatat ctgcttaaat
     3541 atttttaact ttgttattag aagaaacaat actacaataa ggcttgtctt ttctattaat
     3601 gcgattaaaa ttgatgtttt cataatcccc ctttaaaaaa taaattttta cacactctaa
     3661 atttattatg gttgttataa attacatatt tgaagtaatc ctatcacaaa actttccaca
     3721 tttaatcttt taattaaata agaaatagtt gtacttctat taacaactaa tactagctag
     3781 cataagcttg taataacttg tagttattat taactttaca attctagtat ttaattttat
     3841 a
//
LOCUS       DAKBNN010000275         3717 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000275 DAKBNN010000000
VERSION     DAKBNN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.281"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            206..1381
                     /locus_tag="OUB02_003628"
     CDS             206..1381
                     /locus_tag="OUB02_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8887349.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYNTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEAEAKLKSECLRKII
                     ECCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1554..2951)
                     /gene="asnS"
                     /locus_tag="OUB02_003629"
     CDS             complement(1554..2951)
                     /gene="asnS"
                     /locus_tag="OUB02_003629"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCT8887350.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3028..3261)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3430..3624)
                     /locus_tag="OUB02_003630"
     CDS             complement(3430..3624)
                     /locus_tag="OUB02_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HCT8887351.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
BASE COUNT         1235 a          617 c          449 g         1416 t
ORIGIN      
        1 tagtaaacta tctgtaaatc ttaattataa taaagtatta atcaataaag ttactcgaca
       61 ttatctaata aattttatct atcacaattt tagattgtta cacacaatat attttgatat
      121 aattaaatca taaacgaagt attccaaata tctaatgata gaaaggaata cttattttta
      181 tgctttattt ttaatggagg cttatatgaa cttagaaaaa ataaaaaaat tttttggatt
      241 tcctctagac aaaaaacaca aagacaatgt tgagaaagaa ctctgcaatt atctttataa
      301 tacgggtaaa atcatttgca taataatagt aatatctcaa ataattatga tgatatcaat
      361 ctcagctaga gaaggaggcc cttttgtcac accaagacgc caatggtatt tttatatgta
      421 tgtaacacta ttttgtgtat cttctgtctt tatgtttgta ttttcttacc tttggaataa
      481 aaaaaaggat tgctgtacgt tttatataaa tttatggata atttatgtga cttttttctg
      541 cctatggggt tgtgtaatta ctctacttga ccagttaggt ggaaatgacc taagtgtatt
      601 tagttatatt atgttagcag ctgcagcttt ctcagtatta aaatttggag aatcagtaat
      661 agtttttgga ggatgctttt tgtttcttaa tttattctta cctgggattc aaactatacc
      721 aggcaatgag tttagtaata ttgtcaacac attttttata acatcccttg ccatttttgt
      781 ttctacaatg ctatatagaa atcgtgtatt tacttactat gataaacttt taattagtca
      841 acaatatgaa gaaatatctc atataaataa tttacttaac gacaaagtta tgacagatga
      901 ccttactaaa atgaacaacc gtaggtacct tgaagatatt ctacaaaaaa aattaatcag
      961 ccatgcaaat caagaaccta taactggaat gatgatagat atagattatt ttaaacaatt
     1021 taatgataat tatggacatc aaaagggaga ctattgtcta caaaatattg ccaaagtaat
     1081 tttagatttt acaagttctg aaaatgaaca tgctatacga tatggtggtg aggaattttt
     1141 tatatgtatt tttaactgta atgaggcaga ggctaaactt aaatcagaat gtttacgaaa
     1201 aattatagag tgctgcaatt ttgagcgtga tgatgttcct ttaaaatgtg taactgttag
     1261 tattggagtt tatacaaaga aaattgaaaa gacaataaca ttaaatgagt ttataagtaa
     1321 ttgtgataag gctctatata ctgcaaaaga acaaggacgt aattgtgttg cattttatta
     1381 aaaagttaca tactattaca taaacctata agtattatat cgcaaaataa aagtaatcaa
     1441 attatatatt tatagtacaa taaaaataac ttgcatatct ctaaaataga taaatatgca
     1501 agtcatttta gtgtttatac ttaaagttat ataggtttta ttatatataa ttattaaaat
     1561 tcagcatttt taggagttct tgggaaaggt ataacgtctc ttatgttaga catacctgtc
     1621 atatacataa ttattctttc aaatccaaga ccaaatccag agtgagtagc agtaccaaac
     1681 tttctaagtt ctaaatacca ccaataatct tcttctctta atcccatttc attaattcta
     1741 tctaacaata catcttctct ttcttctctt tgagaacctc ctacgatttc tccaactcca
     1801 ggaactagta aatccattgc tctaacagtt ttaccatctt cattcattct catatagaaa
     1861 gcttttatat cttttggata atctgtaaca aacactggac aattatatat ttgctccgtt
     1921 atatatcttt catgctcagt ttgaaggtca catccccact caacaggata ttcaaattca
     1981 tgaccagatt caattaataa ttccactgct ttagtatacg ttattctaat aaactcagag
     2041 tttactatct tatttaatct ttctaacaat cctttatcta taaaactatt aaaaaattcc
     2101 atttcttcag gagcattttc taatacatag cttataacat attttatcat atcttctgca
     2161 agttccatgt tatcttcaag gtctgcaaaa actatttctg gctctatcat ccaaaattct
     2221 gaagcatgtc ttcctgtata agagttttca gctctaaatg taggtccaaa agtataaacg
     2281 tttctaaaag aaagtgccat tatctcggca tttaattgac cactaactgt taaattagcc
     2341 tcttttccaa agaaatcttg agtatagtct attttgccat cttcagtttt tggaatatca
     2401 tttaaatcca tagttgttat tctaaacatc tctcctgcac cttcacagtc actacctgtt
     2461 atgattggag aatgtgcata tacaaagttt ctttcttgga agaatttgtg tattgcatag
     2521 gcagctattg atcttactct aaatacagct gaaaaagtat tacttcttgg tcttaaatgt
     2581 gctatcgttc ttaaatattc aagcgtatgt ctcttctttt gtagtggata cgaactatct
     2641 gattcccctt ctacaactat gctttttgca tgtatttcga ctggttgttt agccccttct
     2701 gtagatacta actctccctc tacaagtata gatgagctta ttgctaattt tcctacctct
     2761 ttaaaatttt ctactttctc atcaaatact atctgcatat ttttaaagaa gcttccatca
     2821 tttagctcta taaatccaaa atttttagat actctagatg ttcttatcca tccagctact
     2881 tttacagtct tccctatgta ctgttctttt tctctgtaca attcttttac tgttataaat
     2941 tccttttgca ttttaaatcc tcctagtata attgttatta atttctttta aatataattt
     3001 caataaaaaa agcccttcat ccctatataa agggacgaaa gactattact ctcgcggtac
     3061 caccctagtt gtcataatga cctcttagtt aagcaatgct taaagcctat aacggtgcct
     3121 tccgtctaag tctacttact aaataagctt tcgattagaa actccaagat gttcttcaat
     3181 ataaacctcg tattgagctt tcaccatcct caactcgcta gaactacatt ttatatctac
     3241 tcttctcttc acagtattta tcttttatta tttctgctaa ttaacaaaat tataccaatt
     3301 tataaatttt tagtcaataa atttatataa ttgatactga ttgttattta aaatataata
     3361 attctacctt acataatgat aataaatact tatcttcatt acttctttat tacttcaatt
     3421 tccaaaattt tataatttac ttccattgtc aacccagtct tttgcttctt cgacaccctt
     3481 aattgaaggt attgaaactt tactatgagg ttttaatttt tcaacatcag cccatgctgc
     3541 tgtcgactca tttccaacta aaagtgaact tttaagtctt ttattatctt taccttttaa
     3601 tgctcttgcc tttttacttt ccaaaactat cacctctatt atttattttg taatagtttt
     3661 agcaatttta caaataaaat tcataagtct aaattataat gatttttaaa ttaacat
//
LOCUS       DAKBNN010000276         3704 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000276 DAKBNN010000000
VERSION     DAKBNN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.282"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            37..705
                     /locus_tag="OUB02_003631"
     CDS             37..705
                     /locus_tag="OUB02_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887352.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            864..1370
                     /locus_tag="OUB02_003632"
     CDS             864..1370
                     /locus_tag="OUB02_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCT8887353.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1498..2514
                     /locus_tag="OUB02_003633"
     CDS             1498..2514
                     /locus_tag="OUB02_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HCT8887354.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTLANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2867..3571
                     /locus_tag="OUB02_003634"
     CDS             2867..3571
                     /locus_tag="OUB02_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HCT8887355.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLGDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1585 a          317 c          651 g         1151 t
ORIGIN      
        1 ggtattttaa ataaattttt taaagggtga ttttttatgg aaaatataac tagttatttt
       61 acaacaatac tatgtgtatt tatatgctta tcatctgtat ttatatttac tcaactagct
      121 agagtattta ttaataagaa gaagattaat caaaaaatta aatcaagaaa cggatttagg
      181 tatgatagag attttattga agccagaaga gaagaaatac atataaagga taacaataat
      241 aataaaaata agagtaacaa taagaaatta aagggagaaa aagtatttaa atacgataat
      301 ggtgatttat ataaaggtga gtttgttgat ggaaagaaaa atggatttgg aatatatatt
      361 ttttcaagta aggaaaagta tgaaggatta tggaaagatg acaagatgca tggaatagga
      421 aaatatactt atagagatgg ttcaatatat acaggtgaat ttaaatacgg attaaaaaat
      481 ggtttgggaa aattaacata tcccaataat gacatttata agggatattt tttagataat
      541 aagaaaaatg gtaagggtgt attatataaa aatgatggaa ataaacaagc aggaatatgg
      601 gaagatgatg aacaatgtaa aagtttagat tttaaagatt taaataataa taaaataaat
      661 aatgtttatc aaaatagatt aaataacaat aaattaaaag tttaattatt ctaataaaaa
      721 aatctttatt tatgatggaa tataggatat atctatgttg taatttgtaa aatcttccta
      781 tatcatataa aattaagtat tgattagtct attacataaa tatagaatat aaatatatcc
      841 atttaataat aaggagaaga gatatgaaag agtatgtagt tgtggattta gaaactacag
      901 gattagatcc atataaaggt tgtgaaataa tagaaatagg aataacagaa ataaaaaatg
      961 aacaaattgt aaaaaactat tcaaggttga taaaacctaa ggggataatt tcttcattta
     1021 taacagaatt gacacatata agtaatgaaa tggtagaaaa tgaagaacca ctagaattag
     1081 ttttacctag atttagaaaa tacataggtg atagaactat aattgcacac aatgcgaaat
     1141 ttgatttaaa atttttaaat tactatctta gaatgttaga tttagaacca ataaacaatt
     1201 atatatgtac agtagagtta ttaaaaaaat gtaaaagtta taaaggaaaa aacaagaaac
     1261 ttgaaacagc ttgtgcttat tacaacatag agaatataaa tgctcataga gctgacagtg
     1321 atacattagc aactgcaaaa ctatttttga aaataaaaga tgaatattaa ttataaatat
     1381 agagaaaatt agtgaaaata tcctttataa aattgaccct taagcttaca attttgttct
     1441 ttgatttatt ttgtagaaca atgtataatg taccttatat gaagaaggga gactaaaatg
     1501 ggttattaca atgataatga taataataaa ataagacgta aaaaaattag taacaacaaa
     1561 gatagtaata aaaaatctaa acattcttat actttggcaa ataaaaaaaa taaaagtcct
     1621 aatacaagtt ccagtaaaag aatgagtgaa aaagtagatt cacagaatct tataaaaaga
     1681 gatagagata gttttacaag agataatgta agacctttaa atacagatta caatcaaaga
     1741 gaaagaagaa gagtcgagta taaacaaaga gagaaaaaat taaaaagtaa aaaaagaaaa
     1801 aaacaaaaaa ggattagaat agttagggca ttattatcta ttatgttagt tgcactaatt
     1861 atattttcag tggcaggtgc aatatttaca gtttcagcta taaaagggtc accagaagtt
     1921 actaaaaagt taattaggtc taaatatata agtagtgagg ttgtaagtat aaaacaaatg
     1981 ccaaatgatt taaaacatgc aatagtttcc atagaagatg agagatttta taagcataaa
     2041 ggagtagatg ttatttctct agttaggtca gtattaaaca atgtgtttac agatacaaca
     2101 caaggtgcaa gtactataga tatgcaagtt tcaaaaaacc tgcttactag taatgataaa
     2161 actatgaagc ggaaaataag ggatatgtat aatgctaagc agatgaataa gattatgact
     2221 aaggatgaaa ttcttgaagc atatttaaat aatatgtatc taggtaaaaa tgcatatgga
     2281 gctgcaaagg gagctgaagt ctactttggt aaaaaggtta gtgaattaaa tttagctgag
     2341 tgtgctatgt tagctggtat gacaaataat cctgcaaggt atattgacca tggagaggct
     2401 aagaagagac aagtgattgt gttgtataag atgcatcagc ttggttatat aactgataag
     2461 gagtatagaa tagcaaaagc tgatgacact ccatttaaat cagaaattga ttaaaattgt
     2521 aagaatatag tatttcaata gtttaaacaa acgaaaatgt aataaaaaag gtgatttgcc
     2581 acccatttgc caccataatt ataatttaat agcaaattgt tttaatgtac aaatccgata
     2641 atataatatt gttttaaata ctacattatg ctaaaattta attgtatttt gtaaattaaa
     2701 aaacttataa tttagttttc tagggttccg cattttataa tggtcaggtc caagagaaaa
     2761 cgcacaattg ttgtgtacac ggaaggataa aagcctggga gatatttaat aatatttcta
     2821 taggcttttt tatttttaca tattttaaat ttgaggaggt tttctaatga aaaaatttgt
     2881 tattgaggat gcattttgga atctatttcc tagtgcaaaa attggagttg ttatttgtaa
     2941 tgatatagat aactctataa aagatgaaga ctataagaaa ataattttga agggagaaga
     3001 agaagcatta aaatacttgg gggactcaga atttagcaac aaccaagtga ttaaagtatg
     3061 gagagaagca tttaaaaaat ttaaaacgaa aaaaggagca aggtcttcta ttgaagcatt
     3121 attaaaaaga gttcataatg gaaataatat aggaactatt aaccctttag tggatatcta
     3181 caattttatc tcattaaaat atgcattacc ttgtggaggg gaggatatag ataaatttat
     3241 aggagatatt aggctaacta gagcaattgg taatgaggag tttatcccac tagggacaga
     3301 tgaaaattca tttccatatg aaggagaaat aatttataaa gatgatggtg gagcaatttg
     3361 tagatgttgg aattggcgtg aagcagtgag aacaatgctt actgaagata ctaacaatgc
     3421 ctttttatgc attgaattag tagatgaaag tagatttaaa gaatttgaaa atgctttaaa
     3481 tgaattagct aaaatagttc aagaaaaatt gggtgggaat tgtaaaattt caattttaga
     3541 tattgataat aaagaggtat cagtggatta atatggaggt gctgttgtaa tggactattc
     3601 tagttataaa gatacttata tttagggtaa gaaggtctat tttagatata acgattatca
     3661 aaggatataa tatagaaaat aaaaatgtag aatataaaaa gcct
//
LOCUS       DAKBNN010000277         3672 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000277 DAKBNN010000000
VERSION     DAKBNN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.283"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(1..465)
                     /locus_tag="OUB02_003635"
                     /pseudo
     CDS             complement(1..465)
                     /locus_tag="OUB02_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            734..>3672
                     /gene="cbpA"
                     /locus_tag="OUB02_003636"
     CDS             734..>3672
                     /gene="cbpA"
                     /locus_tag="OUB02_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HCT8887356.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDANAGNAGGDANAGNAGGDTNAGNAEGDANAGNAGGDANAGNAEGD
                     ANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAEDPTSVGDFKISGGEINVDF
                     KFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTLT
                     PDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVPE
                     GGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIIIN
                     GGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHGAGIGGGYYTTT
                     KNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVTG
                     GTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMNK
                     ISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDKFTGSEVSVN
                     LVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEEDV
                     EKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTKQVD
                     AGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKPT
                     DTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTASI
                     EWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDINI
                     DTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDGN
                     PDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDPDN
                     GGNSKPDS"
BASE COUNT         1376 a          426 c          752 g         1118 t
ORIGIN      
        1 aagtataagt ttgtacttta taatttattt ctactaaatt attttctttg aaaaatactt
       61 catcagtata gttttctagc tgaccaaaca tagttttatt tttcatataa tgaccataga
      121 ctattaaact cttatcattt tcaaaattat ttctataatc catgaaaata ctacctaaag
      181 gaagataatt tttataaaaa tcatgagtta ggtagaaatc attgttatat ccttgtacta
      241 caggatagtc tatatttacg ttgtttattt taagccagaa tctataatca ttatttttat
      301 cataaagttc ttgatgtttg ctattattga tagtgttttc tctgagttcc gtatatactt
      361 catcagcttt ttatattcag ataattttat gtaaatcgaa aggctagaga aagttagcgt
      421 aataatgcaa ataatattta ttaatatttt tactagtttt ttcaattaat taccccttgt
      481 attattatat aaaagattaa ataaatttta tataattaaa atttttattg tagttattta
      541 gcaaataatg aatacttata atatataagg ttgaaaaaaa ctgtatattc attgacaaat
      601 ataagtttta agtatatatt aaaaattatt agagtgtata taaggatgta tacagtgtat
      661 attaagaatt attagagtgt atataaggat gtatacagtg tatattaaaa attattaagg
      721 ggagggggaa tttatgcaac aaaaatgtaa aaagaaagca ctatcaatga ttttaagtat
      781 ctcaatggga cttggatcag tagcatcatt taatgcgtca aatactttcg cagatactat
      841 agaagaaagt actaatgcag gaaatgcaga aggagatact aatgcaggaa atgcagaagg
      901 ggatgctaat gcaggaaatg caggagggga tgctaatgca ggaaatgcag gaggagatac
      961 taatgcagga aatgcagaag gagatgctaa tgcaggaaat gcaggaggag atgctaatgc
     1021 aggaaatgca gaaggagatg ctaatgcagg aaatgcagaa gaagattcta atgcaggaaa
     1081 tgcagaagaa gattctaatg caggaaatgc agaaggagat atgaatgcat tagttgtgga
     1141 agaagccact gcagaagatc ctacatctgt aggagacttt aaaattagtg gtggagaaat
     1201 aaatgtcgat tttaaatttg caagtggcgt tttaactatt cttagtggaa aacaaatgac
     1261 aattcaaaat aataatccaa atactccaac aacacatagg attgttatac ctaaagatgt
     1321 aaaagctgaa ttaatatttg atggagtaaa tattaaaact gcatctaata gtccatttac
     1381 tttaacacca gataaagatg gtggtgcata tgcacatgtt atattacaag atggtagtga
     1441 aaacaagcta gagtcaggtg atgtatatta tccaggtatg agagcaggaa aaggtactac
     1501 tctaattatt gatgattctg ttgtaaatag agatattgaa ggcgagttta tagttcctga
     1561 gggtggaaaa attcctagag atgtaactct agaaaatgga gacaagttaa aaaaaggaga
     1621 tcgccttaca ctactagata gtaataatag aggaagttta aaagttgttg gaaattatta
     1681 ttctgcaggt attggcggtg gaaatactga aagtggagga aatattatta ttaatggtgg
     1741 cgctataact gctataggtc aagaatcgag tcgtaataca ggatatggag ctggaatagg
     1801 tggaggcggt aatggtagtg gtggagatat cacaatcaat ggtggtacta taactgctaa
     1861 aggtgcatac catggagctg gaataggtgg aggatactat acaacaacaa agaatgcatt
     1921 aagtccctca gtagtagatc ctagcacagg ttctggttat agtgggaata ttacaattaa
     1981 tggtggactt tcctattcat atggaggtgc acatggtagt gcatttgggg atggatgtgt
     2041 taatccagga acaaatagag gatatcgtat acttgtaact ggaggaacga ttttacctta
     2101 tatgacagga gtgggagatt ggatttttga cctaagtgct acaacgggag atgttatagt
     2161 aacaggtggt tcattaaaag caacaacatt caaaagttta ggtggagcag ttgcatacgg
     2221 tgatttagaa aagaaaacaa aagtttttat gaataaaata tctctaacgt atttagggca
     2281 agataaagtt agtactacac tagttgatga ttttaaaatg ctaattgatg gaaaaattta
     2341 tccttatgga gcacctagtt atacagatgc tcaagggatt ctttacttat ggttctctga
     2401 taaatttact ggatctgaag tatctgttaa tttagttgtt atagataaaa ttactggaga
     2461 agttctaaaa ccagatgatt tttatattaa agatataggt tcaggttctc aatttttaaa
     2521 gcaatatgaa gaatttacta ttgatgatac tccaccatat ttgattatta aaagatatga
     2581 tggattacca tttgaagaag acgtagaaaa atcattttta gattggctat taaaagatgg
     2641 tggtattcca ataactactc ctaaaggtga aaaactcaca gataaaaaat ttatgactat
     2701 tagttctcaa cgtttaaatg aggaaacgat ggaaccagat ataaatttag gaagtgatga
     2761 tcttacaaaa caagttgatg ctgggaaata tcaattaagc attacatcaa cacagttttc
     2821 gagtatatat ccttttaagg atgcttattg ggggcataga gcatactata aggcagaaat
     2881 tattcctgct gatacagaaa cttcattagt tgtagaagag tctaaagcta aaactgatgt
     2941 ttttaaacca acagatacgt ttacattaaa tgttactatt tcaccagata agaaagaagg
     3001 accagattgt gcctctccaa gaggttacgt acaattctat attaatggaa aggaatataa
     3061 agatccagta gaattaaaaa ctcataataa gggtagtgag agtcataatt atagtactgc
     3121 atctattgaa tggagacctt tagatagcaa tcatcatatt tttactactg aacaaactat
     3181 tactgtaaaa tatattggag atgatataaa ttacattgaa ggtagtgagg atgaaaagaa
     3241 gttaaagtta gatatgacaa atgtagatgt agatggggat ggaataccgg atataaatat
     3301 agacactgat ggtgatggaa ggccagatat caatatagat acgagtggtg attggaagcc
     3361 agatattaac atagatactg ataatacagg tgaatggaag cctagtacag aaggtggaaa
     3421 tggagatggt atttggaaac ctgataaaaa tatagatacc aatggagatg gaaaccctga
     3481 tacagattat aatcgtccag caatagatac agataatgat ggagtagatg attattggaa
     3541 gcctgataaa aatgtagata ctggctcagg aggttatgat acaggtaatc caaatctaaa
     3601 taagccagat ccagataatg gaggaaatag caagccggat ccagataatg gaggaaatag
     3661 caaaccagat tc
//
LOCUS       DAKBNN010000278         3665 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000278 DAKBNN010000000
VERSION     DAKBNN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.284"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            178..1758
                     /locus_tag="OUB02_003637"
     CDS             178..1758
                     /locus_tag="OUB02_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCT8887357.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1932..2378
                     /locus_tag="OUB02_003638"
     CDS             1932..2378
                     /locus_tag="OUB02_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCT8887358.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2399..3442
                     /locus_tag="OUB02_003639"
     CDS             2399..3442
                     /locus_tag="OUB02_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCT8887359.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1476 a          338 c          690 g         1161 t
ORIGIN      
        1 tatatggtat aataaattaa ataattgttt taattcaaaa ttttttgtta ttttaaatta
       61 tttcaaaact cttagtttta gtgattttat ttatgatatt tatgggatta aaaagatttc
      121 aatagatatt tttggtaagt atttaagaag tctttaactt tataggagtg ataatttatg
      181 ctaataggta catctaaatt aagcagaaat gttgatactg catgtgtaga agaagtaggg
      241 attccaatga ttgtattgat ggaaaatgcg gttatatctg caatgaaaaa tatggatata
      301 gacatgtata atagttacac agttgtatgt ggtgttggaa ataatggagg agatggtctt
      361 ggaatagcaa gacatctaaa tacatatgga aaagaagtaa acgtattttt agtaggtaat
      421 cttgaaaaac taagtgaatg ctctaaaata aattacaatg tacttttaaa tatgggaatt
      481 aatattataa atattaataa aaatgattta agtgaaaaaa attatggaga tgaaaatgaa
      541 gtagataaaa ttattaaagt tgagaaacaa aatattataa aaaataaaaa tttaacaatt
      601 tttaaagata gagtatctaa aagtgatgtt gtagtagatg ctatatttgg gactggatta
      661 aaacgtgata taactggtat ttttaaagaa gttattgaca tagtaaacga taatagtaaa
      721 aatacatatt ctatagatat tccttcaggt atagatagcg ataatggaaa tgtattagga
      781 acttgtatta gagcaaacaa aacgattagc tttgagtttt ataaaagagg ttttctaaac
      841 tatgacacta gcacacttat aggtgatgta gttgtagaaa agataggtgt tccagatttt
      901 attacaagaa aataccatga tgaagagttc ataactgata agacttttgt aaaaaataat
      961 attagaagaa gagataagca tggttttaaa agtgattttg gaaaagtatc tatagttgca
     1021 ggttcaaaag gattttatgg tgcaagtttc atagcaactg aatctgctgt taagagtggg
     1081 agtggacttg taacattaat tagtagtgaa gatgtgcaag aaaaggtatc tattagactt
     1141 acagaagcaa tgactttaaa ttttgaagaa gaaagattaa ataaattact taattctagt
     1201 gatgcaattg gttttggtcc aggtatggga gataatagtc aaacatttga taaattactt
     1261 aagatagtag aaaattctaa ttgcccaata gtattagatg cagatggatt gaacgttatg
     1321 aaggacagat gttataagtt tttagaatgg aaaaatagat ttgtaattac acctcatttg
     1381 ggagaaatgg ccaggttgac aggagattca attgggtata taagagaaca tagagtagat
     1441 gttgcaaaag aatttgctca aaaatataat ctggtggtgc ttttaaaagg ttatcaaact
     1501 gtcataacag atggtaaaaa aacatatata aatcctacag gaaatagctg tatggcaact
     1561 ggtggaatgg gtgattgttt gcttggaatg attacttcat ttataggtca aggtatggat
     1621 atattggaag ctacagttag tggagcatat atacatggct atataggaga taaattgagc
     1681 aaagcaatgt atacggtaaa tgctacggat ttaataagta acatttcatt aacaatgaaa
     1741 gagttgttgg atatataatt ggtgatattt gatactactt tgtaacaatt ttaacaaagt
     1801 atgttgatat aatactatta attaatttat ttattttatt gttttgtaat ttaaatttat
     1861 attcaaaaac attgtataaa catataattt atatagggtt ttataattca tatgaaaaga
     1921 gggtagatag tatgaagtta atatggttga tagtagcagt cttatttgga atagcagaaa
     1981 tgttaacacc aagcttaaca ttgatatggt ttagtgtagg agctgtaata cttatatttt
     2041 taagtagctt tatagagagt attttcttac aaattttaat atttgcagta atttctatag
     2101 caatgttagt cgtagctact aaaaaaatag taaaaaaaga taaaggctat aaatctaaca
     2161 ctaatttaca agcaatgatg tctaaaaaag gtattgttac agaagagatt tctccaaata
     2221 acacaggtct agttgtagtt gaacatgaaa catggacggc tatttctata gatggtgaaa
     2281 agatagaaaa aggttctaca gtagaagtat taaaaattga aggggtaaaa ttagttgtta
     2341 aaaaagtaga cgccacagtt aatgtgacaa accaatagaa taaaggagga tttatagtat
     2401 gggtactaaa attgttttaa gtattgtatt aatagtagta gtcgtagcaa taagtttaac
     2461 ttgtataaga gttataaaac aatctaaagt aggtataata atgagacttg gtaagtttca
     2521 aaaagtagct gaaacaggtg tacatttctt aataccattt ttagataaaa tggcatatgt
     2581 aattgacctt agagaaattg taatagattt tccacctcaa ccagttataa ctaaagataa
     2641 tgtaactatg cagattgata cagttgtata ttataaagtt acagacccag ttagatatgt
     2701 gtttgagata gctaatccaa tagctgctat tgaaaattta acagctacaa cactaagaaa
     2761 tataattggt gaacttgatt tagatgaaac attaacatca agagatataa taaatgtaaa
     2821 aatgagaact atccttgatg aagcaacaga taaatggggt ataaaagtaa atagagtaga
     2881 gttaaaaaat ataatgcctc ctcaggatat tcaagttgca atggaaaaac aaatgagagc
     2941 agaaagagaa agaagagagg caatacttca agcagaaggt aataagtcag ctgcaatact
     3001 tcaagctgaa ggtgaaaaac aatctgctat attaacagca gaagccaaaa aagaagctat
     3061 ggtacgtgta gcagaaggtg aaaaagaatc tgctatatta gtggcagaag gtgaagctga
     3121 agctataaga cagacagcta ttgctaaggc acaaggtgaa gctgaaatga taaaaagaac
     3181 tcaaatggca acagcagaag gtttaaaatt agttttttca gcaatgaaag aggctgatat
     3241 agacaataat attctagcat taaaatctat ggaagctctt gaaaaaatgg ctgaaggtaa
     3301 gtcaacaaaa ctggttttac cttcagaagc agttaatttc ctaggaacat tcaaaggaat
     3361 aaaggaagtt atgagtgatg ataacaaaga agtacttgat ataaaagaag ttttaaatga
     3421 taatgaatca ttaaaaaaat aaaagagttt tgttatcata ggaaggatat ggaaattaaa
     3481 ggtcgaatta attaaatata gcacaaaaat gcacgttgaa aagaagagga cttataaatt
     3541 tttaagtctt cttctttgtt tttattagaa agatgaaatt ataaaaataa attaacagaa
     3601 tctttataca atttatcctt tactaatgta aaaagtgtta tataatatta tataaaacat
     3661 atagc
//
LOCUS       DAKBNN010000279         3584 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000279 DAKBNN010000000
VERSION     DAKBNN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.285"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            37..621
                     /locus_tag="OUB02_003640"
     CDS             37..621
                     /locus_tag="OUB02_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887360.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
     gene            661..1464
                     /gene="proC"
                     /locus_tag="OUB02_003641"
     CDS             661..1464
                     /gene="proC"
                     /locus_tag="OUB02_003641"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCT8887361.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(1545..2720)
                     /locus_tag="OUB02_003642"
     CDS             complement(1545..2720)
                     /locus_tag="OUB02_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HCT8887362.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            2987..3463
                     /locus_tag="OUB02_003643"
     CDS             2987..3463
                     /locus_tag="OUB02_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HCT8887363.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
BASE COUNT         1316 a          461 c          604 g         1203 t
ORIGIN      
        1 ttatgaaaaa attaaaatta tttaggggtg atggttttga ataaggacat aggagcaaaa
       61 ataaagcaac tcagaactca aaagcaaatg acgttgaagg atatgagtga aaaaacaaac
      121 ttgtcaattg gttttttatc acaacttgaa agaggattaa caagtgtggc aacagattcc
      181 ttaggaaaaa tagcaagtgt actagatgta gaattaactt acttttttat gaagccaaaa
      241 gagcataaaa gagctgtatt aagaagttat gaaaaagaag tatttgatgt agaaaactca
      301 acatttatac attatcattt atcatctagt ttaaaggaaa aaacaatgct accaagatta
      361 attgaaattc ttccaagtaa gagtagtgag gaaatttgtt gttacgtaca tgaaggtgag
      421 gagtttgtgt atgtattaga gggtactctt acagtgtttt tgggagatga acaaatagaa
      481 atgtatccag gagacacaat acactataat agtgaaaaga acaatcataa ttgggtcaac
      541 tatactaata aggtagtgaa aattttggta gtaagtatac caaatccatt tgaaaagtct
      601 gatgcagtta aagaagctta aattttaacg attactatta gtcaataagg aggtactaaa
      661 gtgaaaaaaa ttggattcat aggtgcagga aatatggcaa gcgctatgat agggggaata
      721 gtaaattcaa aattagttga acctaatatg gttatagcat cagcatatag tcaaggaact
      781 ctcgatagaa tagatgcaaa ttttggaata aatactacaa aagactcgaa ggaagtaact
      841 agaacctctg atatagtaat tgtagctgtt aaaccagata tatatgatga tattttggaa
      901 gagataaaag actttataga tgacaataaa attatagtta caatagcagc aggaaaatct
      961 ataaaagata tagagtctat tataggagaa gataaaaaaa tagttagaac tatgccaaat
     1021 actccagctc ttgtaaatga agccatgtct tctctttcta taaataaaaa tataaacaaa
     1081 gaagacttag aagcagttac agaagtattt aattcttttg gaaatactga ggtagtacca
     1141 gagtatctta tagaagctgt aattggtgca agtggttctg caccagcata tgtattttta
     1201 tttatagaag ctatagcaga tgcagcagta attgcaggaa tgccaagacc acaagcatat
     1261 aagtttgcat cacaggctgt tatgggttct gcaaaaatgg tacttgagac aggaaaacat
     1321 cctggtgaat taaaagatat ggtatgttca cctggtggaa ctactataga agcagttaaa
     1381 gtacttgaag aagaaggttt tagagcttct gtgataaaag caatatgtgc atgtatagaa
     1441 aaatctaaaa aaatgagtga gtaataaaaa tttattgtaa ataaagagaa ttgcctttta
     1501 tagaaaattt ccattttagg caattctttt tatttgttta atttctaaaa taagcctgaa
     1561 agccaatttt ttattttttc aaagaaacca actgagtcat tattatttga ttgattttct
     1621 gaattatttg ttgtgttgtt ttcatttgtt tgagaactat cgttattttc attattactt
     1681 gaagtatcat tttttgagct atttgtatca ctatcattgt tagaattagt attattttgt
     1741 ggatttattt gaacaccttc tgtagaattt attacagcat cagagccaag agactcatca
     1801 ttagtgttat ttaagattcc caaatctttt ttatctccgc ctgagaataa accagagaac
     1861 cagtctccta ttccactaaa ccatccacca atagtatcta gtataccaga acccttaaca
     1921 ctttcaccta atttctttaa ttgttcattt acattatctg atacattatc tagtgtattt
     1981 ttcatgctat tgtaatcgta attttggtca gctatttttt tcattacacc tttaatttgt
     2041 tctttttgtt ctggtgtaat agtaatattg taattattta caacattatt tattgtattt
     2101 gctatttgat tggtatcttt tgtaccattc tttacaattt ctgtttttat atcattcatt
     2161 acacctgttg ctttttcttg ccctatatca tctcctaagt cacctgtaac taccaattct
     2221 tcagaagcaa tttctttctt agtttcatca agtttctttc ctgaagcatc ctcaaatgct
     2281 ttcatgattc ctgtcaatgc tccagtacca cttacaggat atggagcagc agcaataaca
     2341 tttgcatctt caagaccagc tgttgtaagg gttgaagcaa tcattgaact agttacccaa
     2401 gttaagtttg ctgttttaac attaattcca tttccttttt tagtaggttc aacgtatgag
     2461 caagagatag taacttttcc aagttgaccc tctgttgcaa caccttgtaa atactgtctt
     2521 tcttctgcat ttgttacatc taaaactaag acttggtctt tttttactcc aaagtaatta
     2581 agcatttgct gcttttgaga ctctgataag ttagctccaa gtgtaactac atttgtaccg
     2641 tctgcataag aaaggttcat actcccaagt acaagcatag tagacacaac aatagaacta
     2701 atttgttttt taaacttcat aatattcctc ctagatttaa catacattac attttcaatc
     2761 tattttttta ttaatatatt gaataaaaat atgtgctaca tttctattat actaaatata
     2821 aactattttt taaatcaatg cgattacaac tttgtaataa aaaagggaat ataaggaaga
     2881 taataaaaaa ataatttttg taagagattg aaaaaacaat taatgtagta tactataata
     2941 tagaaatgat tatgataatg ttatagaatc ggaaggagaa aaataaatgt caaaagaatt
     3001 agtaaaagat agagtaataa aatatttaat agaagattta ttagtacctc aagatatgat
     3061 tgatacaaat gttgagttgg cagaatttga agaaggtgca gaaggaattt tagatattgt
     3121 tgtaaatgtt aaagatgaag aagattatta tgcacctgta atgatagtgc aatgtttaga
     3181 tgaagatgtg gagttagaag gagaagtttt acaaaaacaa atagaatttt tagaagaagt
     3241 ggataatata actatgtcag gtagattggt tttaactaat ggagatgcta tgatgtacgc
     3301 tgattggaga ggggaagaat acgatactga ggcagctctt ccaacttatg atataatggt
     3361 aaaagagttt catgaaatgg aacaacaggc aaaagattta gaagaacatc atcaccatga
     3421 tgaaaattgt ggatgtggat gtaatcatca tcatgaaaat taaatattga gcatatatat
     3481 aaaaaaagac tgtttagtaa acagtctttt tttagacata tatcattcaa aaaactaaga
     3541 atataacaac atataataaa ataaattata tatataagga ggat
//
LOCUS       DAKBNN010000280         3557 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000280 DAKBNN010000000
VERSION     DAKBNN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.286"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     repeat_region   357..1635
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=357..385
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(1831..2070)
                     /locus_tag="OUB02_003644"
     CDS             complement(1831..2070)
                     /locus_tag="OUB02_003644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887364.1"
                     /translation="MECYLKRTLIIFSEAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            2282..3553
                     /locus_tag="OUB02_003645"
     CDS             2282..3553
                     /locus_tag="OUB02_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCT8887365.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1387 a          473 c          427 g         1270 t
ORIGIN      
        1 ctgtatattt attaagtgta gttctagttc ctattgtagt agcattaaat attttttaat
       61 ttttattttc aaatggcatc tccttatatt taaattatag tatgaatatt cataaatttc
      121 aatctattta taaggtaatt attttttata tctaatatct tgtcattaag ttttattata
      181 tatatttata aatttttcaa tactttttat aattattcta cttttatatt ttaatcatta
      241 aaactaaagt caatgttatc tggtaattat ttactacaaa tgctaaaaaa taattatatt
      301 ttaaaggaga aaatgctgta taaattattg gggtatttta cctatacttg ttgcaaattt
      361 acataccact tagttaatat aaaacaaatt tcaaagagat tggacaacga ataaaaattg
      421 caatttatat accatatagt taatataaag catatttgtg tcgatatata gttctaacta
      481 agtataagat ttacatacct catagttaat ataaaacaca tattttacat gtttgaagat
      541 tttatcagaa accctattta cataccgcat agttaatata aaactattaa tagtgctatg
      601 ttgtttattt gcattaacaa gatttacata ccacttagtt aatataaaac ttgtaattta
      661 gataaatgtt ctgttggaga tttaaccaat ttacatacca cttagttaat ataaaactgc
      721 aacagttcag caaggagcta caaatatgta taaatttaca taccacttag ttaatataaa
      781 acaatatact atatttatca actgatgaag tgttgcaata tttacatacc acttagttaa
      841 tataaaactt aacgaaatta aaattgaatc taataattag ctataattta cataccactt
      901 agttaatata aaacggcttg caaaccattc gacaatgcta tttattattt catttacata
      961 ccacttagtt aatataaaac tgaaactttt aacactctac gccatctcta aatattattt
     1021 acataccact tagttaatat aaaacagttg aatatcattt taaattaaaa cttacatatt
     1081 tatttacata ccacttagtt aatataaaac ttataataag tataaccaac tatgcaattt
     1141 gaaaaagaat ttacatacca cttagttaat ataaaacttg tagacttcaa ccctacaatg
     1201 ctagtaggtt cagtatttac ataccactta gttaatataa aacctgtacc tattttaaca
     1261 ggaaatctat tgcaatcttt atttacatac cacttagtta atataaaact agtctcaact
     1321 ttcaaataat cttctttttc tatcagattt acataccact tagttaatat aaaacatcct
     1381 agtcttgatg ctggagataa tattgtaatt catttacata ccacttagtt aatataaaac
     1441 gaccaagtac gtgttttaaa gaaaaaacaa gaagttattt acataccact tagttaatat
     1501 aaaacacaat aaaagtcagc tcgaaatgtt catggaaaac aatttacata ccacttagtt
     1561 aatataaaac gctataataa aaaacagatg gggtggagct tctttgattt acataccact
     1621 tagttaatat aaaaccccaa aataaactaa gtattttcag tacttacaca catacaactc
     1681 tctcaaattt gcagtgaacc atgagtagtg caattgataa catttatcac acaccctcaa
     1741 tcccttacat tgcaactgtt aaaccctact ttatcaaaaa tatcgctcac tgcaaaattt
     1801 atatattttt attatatcat aaaattcaaa ctataaaatt aaaatggaat aatttatcaa
     1861 tttaagaaca cctacatatt tgtaaatact tactatatta tctattctaa taattcttct
     1921 tctagctgga actaatataa aaatcatctc tgaaagatta ggacatacta atataaaaat
     1981 aactatgaat agatattttc ttgttttaaa cgatatggat aaaaaagctt ctgaaaatat
     2041 aatcaaagtt ctctttaaat aacattccat tttatgtaag taggcattat atttttctta
     2101 aatgtcactt aaatgtcagt tatatataat aatagtaaac aactgtattg cactaatatt
     2161 tgctattatc taatagtatt tatagtttag atttgatata atacatgtgg taaaattttg
     2221 atttgtaaaa caaaatttta cctacatata ttaaaacaaa tttaaaacta aggagaccta
     2281 tatgttaaaa gtattaaaag gaaatataat ttttacaaaa acttcagaaa ctttcacagt
     2341 atttgaaaat agttatatta tactggaaaa aggtaaagta aagggaatat ttaaagagat
     2401 acctaaggaa tacaaaaacc taaaagtagt agattacact gataagctta taatacctgg
     2461 tatgaatgac ctccatgccc atgcctctca atttaaaaat cttggaatgg ctatggataa
     2521 agagcttttg ccatggcttg aaacttatac atttccagaa gaatcaaatt ataaagatat
     2581 agaatatgca acaaaaatgt ataaaaaatt tgtaaaagaa ctttggaaat ctggaactac
     2641 aagaatagca gtgtttgcaa cagtacataa agaagcctct atgaaactta tggatatatt
     2701 caaagaagtt ggtcttggag catacgttgg taaagtaaat atgaatatga attgccctga
     2761 tactttatta gaaaacactc aggattctat tactgataca gaagaaatta taaataagta
     2821 tagtgataca gattctattg taaaaccaat aataacacct agatttatac caagctgtac
     2881 aagtgaactt ttaaaaggtt taggagattt atgtttaaaa tataatattc ctattcaatc
     2941 acacttatct gaaaattatg atgagattga atgggttaga agtctagaac ctgaatctaa
     3001 attctatgga gatgcatata atagatataa tttatttggt caaactccta ctttaatggc
     3061 tcattgtgtt cattgttctc aggatgaaat agatttaatg agagaaaata atgttatggc
     3121 agttcactgt ccagcttcta actttaatgt tggtagtggt gctatgccta tcagaaaatt
     3181 aatcgataat aatattagat tagcacttgg atcagatata agcggtggac atacattgtc
     3241 aatatttaaa gcaatggtat ctgcaataca attatctaaa ttatattggg ttaattctgg
     3301 taagaagtat aatttcttat ctttatctga ggctttttat attgcaacca aaagtggtgg
     3361 aagtttcttt ggaaaagttg gtagctttga agaaggttat gattttgatg cattgattat
     3421 tgatgattca aatttaaatc atgacaatta ttctatactt gaaagacttg aaagatttat
     3481 ctatgttggt gatgatagaa atatcattca tagatatgta tgtggaaaac taattgaaga
     3541 acctaatata taattat
//
LOCUS       DAKBNN010000281         3533 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000281 DAKBNN010000000
VERSION     DAKBNN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.287"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            258..2615
                     /locus_tag="OUB02_003646"
     CDS             258..2615
                     /locus_tag="OUB02_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCT8887366.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMVLAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2701..3018)
                     /locus_tag="OUB02_003647"
     CDS             complement(2701..3018)
                     /locus_tag="OUB02_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCT8887367.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1382 a          366 c          548 g         1237 t
ORIGIN      
        1 attatgtata gatattaata agaattgttg aatttatctt gccttaactt tatttactct
       61 tttatattaa gaaaaactta actaaaatat agcgaaattc ataaaaattt aggaaatctt
      121 aagaattata atatttaaat ctttagattt atattttatt atataaacta tagaataata
      181 aaatataaaa attgagaatt ttataatata tttgtagatt tgagtataaa aaagtgctaa
      241 gttgaaagga gtaaaatatg gataaggtta atttagaaag gtatagttgt gatataaatg
      301 ttggtttaaa taaaaatcaa cttcaaagta gaatagatga taatttaatt aatgcatttg
      361 ataatggaaa aaccaaaaca tataaacaaa tttttaaaga caatatattt acacttttta
      421 atctcataaa tattgtttta gtctgtttat tagtttcagt tggttctttt aaaaatacct
      481 tatttatatt tattgctgta attaatacta ttataggagt aatacaagag ataagagcta
      541 aaagattatt ggacaatcta tctgttatag tttcaaataa ggtatctgtc ataagagaaa
      601 aagagaaaaa agagattgat gtacaagaat tagtattaga tgatattatg attttaaaaa
      661 caggtaatca aatttgtgca gattcaaaag ttttaaatgg aaaattagag gtaaatgagt
      721 ccttggttac aggtgaatct gatattatta taaaaaataa aggcgatttt ttatattctg
      781 gaagttttgt agtttctgga gaagcttttg taagagttga aaatatagga aaagataact
      841 atgcaaataa gatagctaat gatgcaaaga tttctaaaaa acataattct caacttagaa
      901 attcattaaa tgcaatatta aaaattgtag gtataataat aattcctcta ggaattatat
      961 tgtttgcaaa acaacatttt ggaaatggag attctatagc tacatctgta gtaggtacag
     1021 tggcagctgt aaatggtatg ataccagagg gattaacatt acttacgagc attgctctag
     1081 ctgtaggaac tataaaactt gctagtcata aaactttagt gcaagaactg tactgtgtag
     1141 agactcttgc tagagttgat acactatgtc ttgataagac aggtacaatt acagaaggta
     1201 aaatgcaagt tgatgatata gtaatgttgg aagaagtgga tattaatgaa ataatgggga
     1261 atatatgtaa ttctctcaaa gatgataatg caacactaaa tgcaataaga gataagtata
     1321 atgttttaga tacgtataaa gctaagaatt taataccatt ttcttcagaa agaaaataca
     1381 gtggggttac ctttgaaaat aaaggtacat atttattagg tgcatttgag tttatattta
     1441 aggaaaaaaa taaaaaactt aggatagaag ttgaaagata ttctaaaaaa ggaaacagag
     1501 taatagtttt agctcatagt gattcttata tggaagataa taatattcct gaagatataa
     1561 agccactagc ttttatattg atattggata aaattagaga tgaagctaag gaaactttag
     1621 aattttttta caatgaaggt gtagatataa aaattatatc tggtgacaat cctataacag
     1681 taagtgaggt agctagaaaa gcaggtctta aaacagctag taattttatt gatgcaacaa
     1741 cacttaaaaa tgatagtgat atatacaaag cagttgataa atacagtgtt tttggaaggg
     1801 taagcccaca acagaaaaaa caaatgattt tagcattaaa aaaacaaaat catacagtag
     1861 ctatgactgg agatggtgta aatgatgtgc tagcacttaa agaagcggat tgtagcattg
     1921 ccatggcatc tgggagtgat gttactaaga atgtgtctaa tttagtctta ttagattcaa
     1981 actttgcatc aatgccaaag gttgttatgg agggaagaaa agtaattaat aacattcaaa
     2041 gagcatcttc tttgttttta gtaaagacaa ttttttcatt cttcttgtcg ttattaacat
     2101 tattcttctt cagtagacag tatccatttg ttcctataca attatcactc attggggctg
     2161 taacagtggg tataccatca ttcttcttag ctcttgaagc aaacaaaagt agaatttcag
     2221 gaaatttttt actcaatata ttaaaaaatg ctttaccagg agcactatgt gtgacagtaa
     2281 atgtaattat tgtttacaat atagttgaat atttaggtta tagttcaagt gtattttcca
     2341 caatgtgtgc tatattaact ggtgtatgtg gtcttttaat attaagacaa caatgtttgc
     2401 catttaataa agaaagatta tttttaatag taagtatgac tattgcattt gttattggga
     2461 ttctattttt aggtggatta ttctcatttg tagcattgag tagagaatta attctcataa
     2521 ctatggtatt agcaatatta atgccaatta tgataaaagt tatgttattt gtcttagatg
     2581 agatattaga gacaattgtt ccagatttaa attaatttta aaatgatata aataagagca
     2641 gttttagttg aactgctctt tttagatgtg tttaatttta tgtaagaata tgtattttta
     2701 ttaatcagct atatatgagc aacagtagtc aacaacttca ttaaccttta agtcaaaact
     2761 tgaatcacta ggaacattaa atttttgccc ttctttataa gttacaaact cattgcttcc
     2821 aggcagtttt acatccatac ttccagcaag catctccatt atttcttctt ctctagtaga
     2881 aaaagtgtat tctcctggca acattaatcc taaagttttt ctttctccat taggcagtat
     2941 tattactctg ctacttacct taccatcaaa ataaacgttt gccttcttta ctgcagtaac
     3001 attcttaaat tcactcatta gataaaacct ccaaaattat ttgtacatat atattatata
     3061 aaaataatgt aaaaaatgct atatatttac aaaaaaattt agtatttatt aaaaaaatta
     3121 ataagaataa aaaagagatg aaattgctta aattgataaa aaatataaaa taaattgcaa
     3181 agtaagcaag aaaagaataa gttgaagagt tatatattat aaatattaaa aattttagta
     3241 ttaataaaag agatattaca ttaatatgaa aatataggag attagaagtg tttgataatt
     3301 acagtacatg ttagatatat cattgtaaat taacatagct ttgaatataa taatgttcaa
     3361 aaggcaatat ttttgaggtc tgaatccaat aataatatag tcttatgatt gtagatatca
     3421 aaaaatataa gttactaaga atacgttttg tagatttata tatgattaat aactaaagct
     3481 ataaataaac aaaataaaaa gagtatgttt caacctatag cttttagatt taa
//
LOCUS       DAKBNN010000282         3508 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000282 DAKBNN010000000
VERSION     DAKBNN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.288"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            376..1245
                     /locus_tag="OUB02_003648"
     CDS             376..1245
                     /locus_tag="OUB02_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8887368.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1471..2337
                     /locus_tag="OUB02_003649"
     CDS             1471..2337
                     /locus_tag="OUB02_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8887369.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPN"
     gene            2529..2726
                     /locus_tag="OUB02_003650"
     CDS             2529..2726
                     /locus_tag="OUB02_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887370.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2729..3172
                     /locus_tag="OUB02_003651"
     CDS             2729..3172
                     /locus_tag="OUB02_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887371.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(3359..3446)
                     /locus_tag="OUB02_003652"
     tRNA            complement(3359..3446)
                     /locus_tag="OUB02_003652"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3410..3412),aa:Leu,seq:caa)
BASE COUNT         1343 a          355 c          618 g         1192 t
ORIGIN      
        1 gacaactctc cctcacatcc tcaaattata gttgtcatta tagaataact tggctgtttc
       61 aaaaatgtga aatcatttta gaaacagctt atttttttat aaaaataaag aaggcaacgg
      121 gaagccttct ttcgtgtaaa aaaagtttaa ttttttgtta agatttgttt gttattttat
      181 attaccttta taatatgttt ttatttggtg gttggcaatg gattttgcac aactggtcat
      241 tattaatgta aaagtggaat attattaata aataataatt acaaaattgt aatctttatt
      301 tattaaataa atgagtaaaa taatatatta atgggtatat aattaagaca atactagttt
      361 ggggaggata aaattatgga ttataaaaca atcatattaa atagaaagtc tgttagagaa
      421 tataagaaca cagatattaa agaagaatat cttagtgaaa ttaagaaata ctcagaatct
      481 tgtaagaaac tagttccaga aatagatgtt gatgtaagaa ttatgaatag aagtgaagtt
      541 tttaataatt tagataaagt agcagggtac aaaggaaata tgatagattc tccaagttat
      601 attataatat tatctgacat aaaagataat tacattgaaa attcagggta cataggagaa
      661 agtataatct ttaaagtaac agaacttgga attggttctt gttgggttac ttttgaagat
      721 ggaaaaaagg taaaagaaaa acttggaatt aattcagata aagaggtaac aggaattata
      781 gctattggat atggagaaaa tgtaagtaat gctaaagtat taaatgctac aaaaactggg
      841 caaaattatt caaaatctga tatgcaagtt gtaacagata aggggtctac tagactaggt
      901 gtagaagatg ttgtttatat gaacgaatgg ggaaatgttg caagtataga aatcttagaa
      961 gaaagagcac ttcttgatgc atttcactat gcaagattag ctccatctac attaaataga
     1021 caaccatgga gatttatagt tgatggaggc actgtagttt tagctgttag aaaagatggt
     1081 catactaatt tatatgaaga aaaaatagac attggtatag ttatgcttta ttttgcaaca
     1141 ataataagtg caactatgtt tgatttaaaa tggaatttag gtactccaga caaagattat
     1201 aaagtgccag aagactataa gatagtagga tattgtaata tataagatag tgtaatactt
     1261 aatttaacta cacttaaatt aatttttata aaaagttcaa taacatttta taatataatg
     1321 ttattgaact tttttattgt ataatacaat ttttatattt attgtattga ttgaaaaata
     1381 cttatgaatt taaatgctta caattttgtg catgaataga gaggtatatt attttattaa
     1441 actgtataat ttgcttggag gtaatatcaa atggaatgga taaataaatt aaatcaagct
     1501 attacttata ttgaagaaaa tctcgaaagt ggagtggatt atgcagaagc agcaaaaatt
     1561 gcatgttgtt caacctttca ttttcaaaga atgttttcat atattgcaga agtaccatta
     1621 tctgaatata ttagacgaag acgtatgact aaggctgcat ttgaacttca gaacagtaac
     1681 atcaaaattt tagaactatc tgagaagtat ggttacgatt caccaacatc ttttaataga
     1741 gcttttcaaa atatacataa catttctcca tctgcagcta gagcaaaggg tgttgtttta
     1801 aaagcatatc ctaagatgac attaagcatt tatgtaaaag gaaatataga aatgcaatat
     1861 cgtattgtag aaaagaaagg gtttcgtatt gttggtataa aagaaagtat gaacatgatt
     1921 gtagaagagt gttttgagaa agttcctaag ttatgggcta aatgcataaa aaatggaaca
     1981 attgataaac tttcagagtt aataaataac gaaccttgtg gattgcttgg agttagtgtg
     2041 tgtacaaata gtaagggttt agattattat attgctgctc caacagataa gcctgtatta
     2101 gaagatactc atgaatactt aataccatca ggcacatggg cagtatttga atgtgtttct
     2161 ccaatgccaa atgctctggc aatacaggaa ttgcaaaaaa gaattattac tgagtggtta
     2221 ccaagttctg ggtatgtgta tgctaattta ccagatattg aactttatcc caatggcgat
     2281 atcaatgctc ctgattatac tacagaagtt tggattccaa ttcagaaacc aaattaaaat
     2341 taataaataa tatggaatta ataaataaca ttaaaattaa taaacaacat gaaattctaa
     2401 aattacaggc tgaaaatagt ctgttatttt tttgtaaata tggaaagtaa gctttacata
     2461 aatggaatat agttgtataa ttatggaaag tagactttcc gtagttgaga tattaaaggg
     2521 ggatagatgt gaaaaataga ttagaagaaa taagaaaaga aagaggcata aaacaagaag
     2581 aacttgcttc tattttgcaa gtatcaagac aaactatagg ttcattagaa aatggtaggt
     2641 ataatccttc tatcatctta gcctttaaga tagctagata ctttgatatg agtattgaag
     2701 aaatatttat ttatgaggag gagtaaaaat gaaaaagaat atgttatatg ttggaattat
     2761 atattttatt tgtggaattt tacttgtttt attggctaca tttactgagt tttcatttga
     2821 ggcctttatt tggggattat ctggagctgc tttaggtcca ggtgtatgta tgattcttca
     2881 atatatgtat tggagtaaac ctgaaagagc agtagattat gaagaaaaga taaaaaatca
     2941 aaggatagag atgaatgatg aaagaagaat tatgttacgt gataaagctg gacgtatcac
     3001 taatcaaatc atgtgctatg ttcttgtaat tcttatattt attgtttcta tattgagtgt
     3061 attttctgta atggtaataa gcaagtgggt tttagttgtt cttggattgc ttattttatt
     3121 tcaatttatt tgtagtgttg ttgtatacaa taaattagat aaaaggttgt aataagttat
     3181 gatataaaat agaaaattat ataaaagtaa attagttttt aaattaatgc tagaagataa
     3241 atttatataa ttatataaat tttaaaaaga cacagtaatt tcaaaaaata acttaaaatt
     3301 taaatcaaaa ataaaaaaac tcctaatcta ttgaataaat agctttagga gtttttaatg
     3361 gtgcccgagg ccggactcga accggcacgg agtattagtc cggtggattt tgagtccact
     3421 gcgtctacca attccaccac tcgggcatgt catgtatatt attatagcaa aaaaatatat
     3481 tgtttgcaac atatttaatt ttaatata
//
LOCUS       DAKBNN010000283         3508 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000283 DAKBNN010000000
VERSION     DAKBNN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.289"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..187
                     /locus_tag="OUB02_003653"
     CDS             <1..187
                     /locus_tag="OUB02_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCT8887372.1"
                     /translation="VGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINA
                     SINILKEGLRLITIQNK"
     gene            314..1483
                     /locus_tag="OUB02_003654"
     CDS             314..1483
                     /locus_tag="OUB02_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCT8887373.1"
                     /translation="MNKLCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1646..2188)
                     /locus_tag="OUB02_003655"
     CDS             complement(1646..2188)
                     /locus_tag="OUB02_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HCT8887374.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2336..2620)
                     /locus_tag="OUB02_003656"
     CDS             complement(2336..2620)
                     /locus_tag="OUB02_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT8887375.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2683..3447)
                     /locus_tag="OUB02_003657"
     CDS             complement(2683..3447)
                     /locus_tag="OUB02_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCT8887376.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSITQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         1201 a          477 c          533 g         1297 t
ORIGIN      
        1 ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa tgtgggtata aaaacgagga
       61 agtgaaggac ttaaatataa gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag
      121 agatataaat gcaagtataa atatattaaa agaaggatta agactaataa caattcaaaa
      181 taaataactt atgtaagaac cgtaggaact actgggatag cctgtggaga attagtaaga
      241 catattttag tatgcaaaat tctgtgaagc aggaaccctg tgactttagt catgggaggt
      301 tcagggtaga aagataaata aactttgtaa agatgaagtt gtagagtttt gcaatagaat
      361 aaaaggtaaa gtggaaagtg ttgcaataag tggagtattt gctccagtaa ataaacaaca
      421 agaagaagaa gttgcaaaat gggttagaga aattttaggg gatattccta tatctttatc
      481 aaatgaaata ggtaatatag gtatattaga aagagaaaat ggagctattt taaattctgc
      541 attgtctcaa gtaggtaaag ctgtatcttt aggatttgaa aaagcactta aagacttagg
      601 tatagatgct caaatatatt tcagtcaaaa tgatggaaca ttaatgaatt tagagtatac
      661 aatgaaatac ccaattttta caattggatg tggaataaca aattctataa gaggagcttc
      721 cttcttatca aaagtaaaaa atgcaatagt attagatgtt ggtggaacta caagcgactt
      781 gggtgtacta tataatggat tccctagaga atcatctatt ccagttacaa ttggaggagt
      841 acatacaaac tttagaatgc cagatgtttt aactattggt cttgctggtg gtacatgtgt
      901 aaaaggcgaa aaagataata taaagatagg tcctgaaagt gtgggatata gaataactga
      961 agatgcaata atatttggtg gagacactct aactttatca gatgttgtta caaaattaga
     1021 tatggcgttt gaagatagaa aaagcaatgt tgaaaactta gataaagagt attgtaatga
     1081 ggtatataag aaggttattt taaaattaga agatgctgta gaccaaatga agactagtca
     1141 agatgatgtt gaattggttt taactggtgg aggaagtata attgttcctg aaaaactaaa
     1201 aggcataagc aatgttataa gacctcctca ttatactgct gcaaatgcta ttggtgcagc
     1261 tttaggacaa ataagtggag atgtagaaaa agtatacagt ttagataaaa tgtcgagaga
     1321 agatgccata gaagatgcaa aaaaagaggc tgtaaataaa gctctaaaag ctggtgctaa
     1381 agaagatact atagaagtat taaatataga agatgttcca cttgcatacc ttcctggtaa
     1441 tgcacttctt ataaaagtaa aagttgttgg tgatttaatt taaagaataa atataagtat
     1501 ttccagaatt atataaatat ttctggactc atttttaaac ttattttatc aataaaaaca
     1561 gcataacttt tgactatatt cttataagtt tccgaacagc cgatatatac ctattttttg
     1621 aggtttatcg gctgtttgtt atcttttatc gcattccaac tatttttctt accgtatttg
     1681 ctgtacgaat tgtaatctta tcacttacat ttgaactggc agtctttgac caccaatttg
     1741 tctttttata atctttacga ctaaaagacc aaaagatagc atttttataa tgatatactt
     1801 tttcatactc ttctttagga tttccaacct catcataaaa ctcgtcataa gataagtcag
     1861 gaaacataaa aataagatta tcatatattt ctttgttgtt atctccccac caattaggag
     1921 cattatttag tatatctttt aactcttctt gtgaaataat aaaaactggt atttccaatt
     1981 caaactgttt ttttatcatt aatcttattg tatttgagat aaccttgctg tcatctacat
     2041 cacttaaaaa gacaacatta ccactattaa ggtatgtaga aacttctgaa taaccaagtt
     2101 ctacaaagct tgcttttaat tcagacataa ttatcttgtt cttaccactt atattgatac
     2161 ctcttaataa tgcgatataa cttttcatat aataatttca cctctaaata ttcacatttt
     2221 tctatgtagt acaaactgga atttgcatct tttataattc tttcaaaact tcttcatatg
     2281 taattttttt aatataaaaa aatcggtgct taatttgtac cgatttttgt ttttcttagt
     2341 aaagaactcg tttttttgct tctgctatag ctctagcagc actttttgct tgcattcttt
     2401 tttcttcttc ttttttaaat tcatttattg tttcatttag attcttatca tgttcatttt
     2461 tttctttatc ttcgttttct ctgtatacta atatataatc tattattgat tcgtttatta
     2521 tctgagatat acttttattt tcattaattc ctattctctt aacttcctta agtagagtct
     2581 catcaatatg aaccgttgtt tgctttgtat tttttgccac cttaatcacc tcttaaaaaa
     2641 aattctaaca ttaaaatttt ttttagtcaa taaattttaa tattaaaatt ctttaaatgt
     2701 tttatttgat tttctaaaat tttctattgt atttatcatc ttatttttta tattttcacc
     2761 tagtacagaa gactttagtc taggattttc gatatggtca taaattggtt ctaaaaattc
     2821 gacttcgaca ggaacagagt taattttacc aataggttgg tatccttcat aagtatcttt
     2881 tgaatttttt attactagtg gaacaatagg gcattttgct ttatatgcta tctttaaagc
     2941 tccacttcta aactccgaaa ccaatgaatt tggttcttta atccatgtta aatctccttc
     3001 tggaaacact gccatacttt gacctgtaag tatattttga gaggcttggg ttatactctt
     3061 tataccctca cgattgtttt ttctgtttac atatacacat cttagtaatt ttgcccattc
     3121 tttaaatata ggtatatttc tccaaactgg ttcatctgca attacacatc caattggtct
     3181 ttctacactt gctgttagta tgaagctgtc aagcatacta gaatgattta caacaaatag
     3241 aagtggttct tttggcagtg tttccttacc aattatatta agctctatgt tcactatatc
     3301 taaagatttt ttagcttgtt tttgcataaa ttcaaatttt tcctttaagc taaatttatc
     3361 aggattcttt ttaattttaa taagcctagg tatagaagat gtaaaaccta taagttgata
     3421 tgtagcaaat tttaagtaat tattcataaa attatctcct taaaataaaa ttatttatta
     3481 ctgtaaagat tttaacataa aataaaat
//
LOCUS       DAKBNN010000284         3501 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000284 DAKBNN010000000
VERSION     DAKBNN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.290"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..2438)
                     /locus_tag="OUB02_003658"
     CDS             complement(<1..2438)
                     /locus_tag="OUB02_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887377.1"
                     /translation="MIFHEVYKIYSKNSLKNNKLLKISCIISIVFATAILLFTRVLTD
                     TYSSEVKRNMAITNGGDIKIQNIEYNKYYFTSEQIDKLEELKGKESIDFQLGSSMNTN
                     ILSNGMIESTELTVVNNIKMQNTYLKLRNENDIVLSQKTANKLNVNIGDKVFLKLHSS
                     VYDDTYFKVSDIIPKRFSLSTAQKEAEVGQEVVGESYVYLPNETKYNVAYINIINKEN
                     VKNIKNEIKNVFKNHFEVRTTDELEQSIIKRISMQLDSINLIGAISLLSTGICLCSTF
                     IVFILNRINDFSTFKALGIKKRSLSFLVFTELMSITIKGIIIGIILGIPITIFYINLQ
                     HINLFNISILSIIKNIFISIVIIIIETSIFSLIPISFVKKIHYEGINRDNSMLSSYKF
                     NFIPDIILVIFLTSISFTIYVKSFLGIFFILGLILLDLLVYSLIYALLKMVCLIKKII
                     KGKYSITFIYLNNDCKTNAFSSSLLTITIIFVLLLFMISEITFAMFSNYEVDNSIKNS
                     IVYQTTLEGKLITDKKLIENDIDYFQYYPIKIDSIESVNNISVNDYIEKIPYENRKDV
                     MNDLNDLSVDLFNTKQTILKSDLEYGNWFKENDKENSIIINNYLHRYLIDYKLGDTVK
                     LRINGKKIDFKVIGFLSPKSLNKGVDLGYININENISPKTLDLSKNQPVIYFFKEKLD
                     NTLLSKLLHNDKYANLEDYNDFAYSMKTYVDDQKSLLLNIIFAVSLSSILLILTSQII
                     IFIRRVLDYQIMYRVGMSKRKLIKIGLLEMIIVSIIQSTLIAIFYELFRFLLISLLQR
                     AYNINYLLILIEFLV"
     gene            complement(2450..3109)
                     /locus_tag="OUB02_003659"
     CDS             complement(2450..3109)
                     /locus_tag="OUB02_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887378.1"
                     /translation="MIIEVSNLSKVVDLLNTELTILRNIDLYIEEGEFVSIMGASGSG
                     KSSLISILSGLDKNYTGNVKVCGENLSKLTDDELAEYRNRKIGIVFQSFNLITSMSVE
                     ENIKLPLIFSDNKDYKIVDKMIKKVGLTSKTKNNIKQLSGGEKQRVAISRALVCSPNI
                     LFADEPTGSLDSTNSHAIMKLFKNINNEYGTTIVMVTHDIQMAEYSDRIISIKDGTII
                     K"
BASE COUNT         1276 a          490 c          309 g         1426 t
ORIGIN      
        1 actaaaaatt ctattaaaat caataaataa ttaatattat aagctctctg caacaatgaa
       61 attagtaaga atctaaatag ttcataaaaa attgctataa gagtactttg gataattgat
      121 acaattatca tttctaataa acctattttt atcaattttc gtttactcat tcctactcta
      181 tacataattt gataatctag tactctcctt ataaaaataa ttatttgaga tgttagtatt
      241 agaagtatac ttgacaatga cacagcaaat ataatattca aaagtaatga cttttgatca
      301 tcaacatatg ttttcataga ataagcaaaa tcattatagt cttctaaatt agcatattta
      361 tcattatgta ataatttact aagtaaagtg ttatctaact tttctttaaa aaaatatatt
      421 actggttgat ttttagataa atcaagggtt ttaggtgaaa tattttcatt tatatttata
      481 tatcctaaat ctacaccttt gttcaatgat tttggactta aaaatcctat tactttaaag
      541 tctatcttct ttccattaat tcttaacttt acagtatcgc ctagtttata atctattaag
      601 tatctatgaa gataattatt tataattata ctattttctt tatcattttc tttgaaccag
      661 tttccatact ctaaatcact ttttaatatt gtctgtttag tattgaataa atcaacactt
      721 aagtcattta aatcattcat tacatctttt ctattctcat atggaatttt ttcaatataa
      781 tcattaacag aaatattatt tactgactct atactatcta ttttaattgg ataatattga
      841 aaataatcta tatcattttc gataagtttt ttatctgtaa ttaattttcc ttctaaagtt
      901 gtttgatata caattgaatt ttttatacta ttatcaactt cataattgct aaacatagca
      961 aaggttattt cagatatcat aaacaacaac aaaacaaata ttattgttat tgttaataga
     1021 ctagagctaa aagcattggt tttacaatca ttattaagat atataaaagt aatcgagtat
     1081 tttcccttaa taattttttt aattaaacat accattttta ataatgcata tatcaaactg
     1141 tataccaata aatctagtag tattaatcct aaaataaaaa aaatacctag aaaggacttc
     1201 acatatattg taaaagaaat acttgttaaa aatattacaa gaattatatc tggaataaag
     1261 ttaaatttat atgaagacaa catactatta tctctattta ttccttcata atgtattttt
     1321 ttaacaaaac ttataggaat taatgagaat attgatgttt caattattat aataactatt
     1381 gaaatgaaaa tatttttgat aatactcaaa atagaaatat taaatagatt aatatgttgt
     1441 aagttaatat agaatatagt tataggaatc cccaaaataa tacctattat aatccccttt
     1501 atagtaattg acataagttc agtaaatact agaaaagata aacttctttt tttaattcct
     1561 aaagccttaa aagttgaaaa atcatttatt cgatttaaaa taaatacaat aaatgtagaa
     1621 catagacaaa ttccagtgct taataatgaa atagctccaa ttaaatttat tgaatctaat
     1681 tgcatagata ttctctttat tattgactgt tctagttcat cagttgttct aacttcaaaa
     1741 tggtttttaa atacattttt tatttcattt ttaatatttt tcacattttc tttgtttatt
     1801 atatttatat aagctacatt atatttagtt tcattaggca aatatacata actttctcca
     1861 actacctctt ggccaacctc tgcttctttt tgagctgtac ttaatgaaaa cctttttggt
     1921 attatatcac taactttaaa atatgtatca tcataaactg aagaatgtag ttttaaaaac
     1981 actttatctc ctatatttac atttaattta ttagctgttt tttgggataa aactatatca
     2041 ttttcatttc ttaattttaa atatgtattt tgcattttta tattatttac tacagtcaat
     2101 tcagtacttt caatcatacc atttgacaat atatttgtat tcatactact tcccaactga
     2161 aaatcaatag attctttacc ttttaattct tctaacttat ctatttgttc acttgtaaag
     2221 tagtatttat tatattctat attttgtatt tttatatctc caccatttgt tatagccata
     2281 tttctcttaa cttcacttga atatgtatcc gttaacactc ttgtaaaaag taatattgct
     2341 gtggcaaata caatagatat tatacaagag atttttaata atttattatt cttcaaacta
     2401 ttcttactat atattttata aacttcatga aatatcaatg tatcaccacc tattttataa
     2461 tagttccatc tttaatactt ataatcctat cactatattc tgccatttga atatcatgag
     2521 taaccattac tattgtagta ccatattcat tgtttatatt cttaaacaat ttcattatag
     2581 catgactatt agtactatct aatgatccag ttggttcatc agcaaagagt atatttggag
     2641 aacaaactag agctcttgaa atagcaactc tttgcttctc accacctgat aattgtttaa
     2701 tattattttt tgtttttgat gtcaacccaa cctttttaat catcttatct actattttat
     2761 aatctttatt atcagaaaaa atcaatggca attttatatt ttcttcaaca gacattgatg
     2821 taattaagtt aaatgattga aatacaatcc ctatctttct atttctatat tcagcaagtt
     2881 catcatcagt taatttcgat aaattctcac cacaaacttt cacgtttcca gtatagtttt
     2941 tatcaagacc acttaatata cttattagag agcttttccc actaccacta gcacccatta
     3001 tagaaacaaa ttctccttct tctatgtata aatctatatt ccttagtata gtaagttctg
     3061 tgtttaacaa gtctacaact ttagataagt ttgaaacttc aattatcatt tttttcatac
     3121 cattatttcc tttcatacaa aacttttaag attgattatt aattaaaaca gagtatatag
     3181 gacacaaaat attagagaaa cttatacaag caagcttttt tgagaatgat atattttcta
     3241 aaactgtaca aatcttaata ggtcattata tttcttatta tacataagat tgcttgacca
     3301 aaagttagta aaattataaa aaataaactt tttcaatgat aaatctaaaa aatataatat
     3361 catcaataat tcggaagtgg gatttaattt aatactaagt tttagcttag atatacagaa
     3421 tctctcccca aaatttatat atttataaca ctttatgtta taaatatatg actctttcaa
     3481 ataaatttca acagaatatt g
//
LOCUS       DAKBNN010000285         3410 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000285 DAKBNN010000000
VERSION     DAKBNN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.291"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(3..842)
                     /locus_tag="OUB02_003660"
     CDS             complement(3..842)
                     /locus_tag="OUB02_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HCT8887379.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            complement(1091..1765)
                     /locus_tag="OUB02_003661"
     CDS             complement(1091..1765)
                     /locus_tag="OUB02_003661"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCT8887380.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            complement(1802..2173)
                     /locus_tag="OUB02_003662"
     CDS             complement(1802..2173)
                     /locus_tag="OUB02_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCT8887381.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            2240..3298
                     /locus_tag="OUB02_003663"
     CDS             2240..3298
                     /locus_tag="OUB02_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCT8887382.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
BASE COUNT         1136 a          536 c          417 g         1321 t
ORIGIN      
        1 aactatttta atatacgtat atctatagtt tttcttcccc agttataaac tgtttttgaa
       61 tcattcatgt agatatcaat tttatttcct tttattgctc caccacagtc ttctgcagta
      121 aacaccatgt caaattgagg tatataaact ttagttccat atggtataac cttagggtct
      181 acagctattg ttccccactt aggttttgtt cctgttgcag ttatactgtg accagtatat
      241 gcagttgcta ccactctcat atttttacca ttgttactac tactgctact agatgatgat
      301 gaagccattc ttcttgaact ttctcccctt gatgaggcta cttgcatttc tacctttgtc
      361 cctttgctta caaccttatt tactggctca gctttaacaa cctttttcac aaattccttt
      421 tctactttct ttccattgtg ataagtttct ttatatatta attcactttt accagacttt
      481 ccttcttctt taaccttaga atcacccttt aataaagagc catcttcaac aactgtaact
      541 tcaaatggaa tgtctttagt ttctacaact gttttttcct ttacatcaat tacttctatt
      601 ttatctccat tagataactt tgtattttca tctaaagtta ctatatcatt tgcatcatag
      661 ctaatgtttt gagattctaa aaggtctcct acattagaac taaatgttga tatagttctt
      721 tcttctcccc ttactactaa tgttacttgt ttattaagcg cagagtatcc cgataatatt
      781 ccaactgata aaacaactgc cattaccgag actttagcat tactcaattt tttaaaattc
      841 atataaaatt tcctccctaa gattttttac actatatatt ttgcttcata aaattctgct
      901 ttgtaagttt ctttttttat ttgtgttttt tgtgtaacta aattgtaact attttttcaa
      961 gagttgtcaa cttgacaaaa aaatagaaat atgcatacat agttttttca accatttaag
     1021 catatttcta ttagttacaa caatgtcatt gtttattgtt gttttgttat cattttgtta
     1081 ccttttactt ttatatattt tctatctgcc aattaatagg ttcttttcct agctttttca
     1141 aaatttcatt tatcttagag aagtgtttac atccaaaaaa gcctttactt gctgacaatg
     1201 ggcttggatg aggtgcttcc aaaatataat gtttacttgt atcaatcaac tctacttttt
     1261 tacgtgcatt acttccccat agtacaaaaa taactgggtc ttcacgctca cttaaaatag
     1321 atattactct atcagtgaaa atttcccatc ctttgttctt atgagaatta gcttcatgcg
     1381 ctctaacagt aagagcagta tttaaaagta acactccttg gtctgcccaa ggcattaaat
     1441 atccattatt aggtataaaa caacctaaat catcttttaa ttctttatac atatttaata
     1501 aagatggtgg agttttcact tgtggttgaa ctgaaaaagc taaaccatga gcttgattat
     1561 ctccatgata agggtcttgt cctaatatta agacttttgt gtctttgtat gaagtgtgtt
     1621 ttaaagcttc atatatattg tgcatatttg ggaaaatttg gcgtgtttta tattcttcta
     1681 taagaaattt tcttaagttt aagtagtaat ctttttcaaa ttcttctttc aataattcat
     1741 cccaatcgtt tcctaagtta accataatta tcaccaattc ctttcaatta tttttattat
     1801 tttatttaga aattatatct tcttgattat cattcttttc atttgtattt atttttatgc
     1861 tcactctatt aggtatacaa actatgcttt gacttggctt gtttataaat ccagtatgta
     1921 cgcaaacctt atcagggcat gaagcatcta ctatctcaac ccctttatca tgaatcttta
     1981 ctatgttata cccccctcta ttctctattt taaattcttc tttggcattt attgaaacag
     2041 ttttatacaa tttattatct acataaatct caatatttcc agcattttta gaatttataa
     2101 catttattat cccataacat gctgtaataa caaccaaaat gcctaaaata agaagaatgt
     2161 cttttttctt cattttttgt cctcctatga aattatgtta taataaaata ataacacaat
     2221 ttcataggag gaatatacca tgaataagaa aaaaatcaca tttatttttg tgatattgat
     2281 tttagcatta attttatttt cattttttag gagttcaagc cagaatacta aatatagtaa
     2341 aactagctac taccttggta cagtaaacga agttactgta tttaatgtaa aagaatccaa
     2401 aagtgataaa atcttaaatg aatgtgattc aattttaaga tatattgaca acaaaatgag
     2461 cactcatata ccaggaagtg atgtttctaa aataaatgat aatgctggta aaaaatttgt
     2521 cagagtttct gatgatacat ttttcgttgt aaaagaagct atacaatatt ccaaattatc
     2581 agatggatat tttgacataa caattggtcc attatcaaat ttatgggcta ttggaacaga
     2641 taaagctaaa gttccaagtg attctgagat acaaaaactt ctgcctctta ttgattataa
     2701 gaacatcata ttggatgaaa aaaataaatc tataaaatta accaaagaaa atatgaaaat
     2761 tgatttaggt gccatagcta aaggatatgc tgctgataaa attgtagcct atttaaaatc
     2821 agaggatgta aaaaaaggtt tagtaaactt aggaggaaat atatttactt tagaagatgg
     2881 taaaaatgat aaacctttta aaataggcat tcaagaccca actagtaaaa atggtgaatc
     2941 cataggaaac atagaaacta caaataagtc tgttgttact tctggaattt atgaaagatt
     3001 tatcgaaaaa gacggaaaaa tatatcatca tatgttaaat ccatttacag ggtatccttt
     3061 tgaaaacaat ttaagtagtg ttacaataat ctctgataaa tctattaatt gtgatgcctt
     3121 atccacttct acatttggac ttggtcttga aaaaggtatg gatttaatta ataaaattga
     3181 taatgtagat gctatattca taactaagga taagaaagta tatttaacta agggtataaa
     3241 agataatttt aaacttacag ataaatcttt ttctattgaa aaattaaata aacaataagt
     3301 ttaaataata attatatcta ttatacatac aaaatacata tgaatttaaa tagttatcac
     3361 atgactaatg caaatttaat ttttatataa aatatggtat atacttaagt
//
LOCUS       DAKBNN010000286         3397 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000286 DAKBNN010000000
VERSION     DAKBNN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.292"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1323
                     /locus_tag="OUB02_003664"
     CDS             <1..1323
                     /locus_tag="OUB02_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HCT8887383.1"
                     /translation="NIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKI
                     QHEAETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKH
                     NKSKLYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMN
                     LTLGVVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSN
                     LLRAVSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLS
                     MTKIDEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQ
                     VLINLIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEE
                     GAKDSRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            1357..2055
                     /locus_tag="OUB02_003665"
     CDS             1357..2055
                     /locus_tag="OUB02_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887384.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            2412..3368
                     /locus_tag="OUB02_003666"
     CDS             2412..3368
                     /locus_tag="OUB02_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HCT8887385.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT         1371 a          315 c          579 g         1132 t
ORIGIN      
        1 aatatagttc ttttaaatta tttttttaca gcaccattat atacattatc tatagcagat
       61 tcaaactata tagtaacact ggttgtattc tcaatagtag gtataataac tagcactctt
      121 acgtctaaga tacagcatga agcagaaact gctgctaaaa gagaagaaaa tacaaaaatg
      181 atatatcaaa taattaaggg atttttaaga ttatcaaata aagatgatat agtaaacaaa
      241 ggtatagaac ttttgtctct tagcttaagt agagatgtaa tttgttattt aaatgtagat
      301 aagcataata aatcaaagct ttacaaaaaa aatactaata ataaggataa aaatgatttg
      361 gatagtgaag atgaaaaagc tgttgcaagt tgggttttat ccaattcaac agtggcagga
      421 aatgacacgg atacacttcc tggttcaaag ggatactata taccaattgt aggaatgaat
      481 ttaactttag gagtagtggg aatatcatgt attgattcaa aattggatac agaagatata
      541 agcttaattg aaacaataat tgctcaaatg gcaatagcgc ttgatagaga aatcttgtca
      601 gaagctaaag aaaataccaa tcttgagatt gaaagggaaa gattaagaag taatctttta
      661 agagcagttt cgcatgacct tagaactcca ctagcaggaa tatctggtgc agtaagtaca
      721 attataaaaa acaagggcac tataggtcaa gatataatag atgaattact tagtggtgta
      781 tatgaagata ctcagtggtt aataagactt gttgaaaatt tacttagtat gaccaagata
      841 gatgaaggga aattggaagt taaaaaacat tctgaactgg ttgaggaaat tatatcagaa
      901 gcgttacaaa aaataaaaaa aagaattgaa aatgcactta tagatattaa tataccagaa
      961 cagatattat ttgttcctat ggatgcaaaa ttaatagaac aagttttaat taatttaata
     1021 gataattctt tgaaatattc aaaggaagat tgtaagatag agataaatgt atatgagaaa
     1081 gatgattatg tttggtttga agtatctgat aatggtcctg gtatatcaaa ggaacttaaa
     1141 aagcatatct ttgacagatt ttttactgga gaagaaggag ctaaagattc cagaaagggt
     1201 gttggacttg gattatcaat ttgtaaatct ataattcaag ctcataaagg tgaaattatg
     1261 gtagaaaaca ataaagataa gggttcaact tttaagttta gtttgcctaa ggaaaatgaa
     1321 tagttttaaa ttttaaaatt agttttggag gaagaaatga gtaaaaaact tatattgtta
     1381 gtagaggatg ataagacaat aagaaaattt atatctactg cacttttaac acaggattat
     1441 gatgtcaagg aggctataac tggaaaagaa ggtatatcta tagctgtctc atattctcca
     1501 gatgttgttc ttttagattt aggcttagaa gatatggatg gaatagaggt aataaaagct
     1561 atcagacaat tttcaaatat accaataatt gtagtttcag ctagagagca agatagagac
     1621 aaggtagaag tatttgatgc aggggcagat gattacttaa caaaaccatt ttccatagta
     1681 gagttattag caagagtaag agtagcattt agacattcac aagtagaagc tcaacaaaaa
     1741 gatgatgtta aatctacgtt tgaagtagat aaattattga ttgattttga taaaagaaaa
     1801 gttattgttg atgatgttga agtccatttg acacctattg aatataatat tctttcatta
     1861 cttgcaaagc atcatggaaa agtgcttact cataatttta taataaaaga aatatggggt
     1921 agtgttatag ggaatgagac taagtcttta agggttttta tggcaacgtt aagacgaaaa
     1981 atagaaaaac aacctgcaaa cccaagatat ataattacag aagttggtgt aggatacaga
     2041 ttaaatgatg aatagttgta atatattaat tattagatta tgtgcttatt aatatatatt
     2101 aaaattatat agtttagtta aaataagaat gaagtgtatg tgcttgttgt cagtttagca
     2161 catgcatttt attttttagc ttttttagta tcaataattt tttagtatca ataatatgaa
     2221 atatgtttta aaataagtat atagtgtaat ctgtaataaa aactttggtt aaataataaa
     2281 tgtatttaac acttacctta taataaattt aatataaata ttaaataata attattaaaa
     2341 tagtgtaaaa taattacagt aaggattata aaaaattttt agaatgtaaa ttgaaattta
     2401 ggagtagatg tatgtatata ctgaatttaa atacaagaga gactattgaa gattttagag
     2461 ataaatttta tgtagcagaa aattcttatc ttatattaag tgctccaaaa aatttaaaat
     2521 tgcttaagga aactttggat atagatgaaa taacttttaa tgattgtctt aaatttgatg
     2581 aaataaccaa acttgattta tttgataatt acgatttttt aagcctaaat acatttgaac
     2641 taagagatgg agaagctgtt atagaagagg taaatatgta tttatcagat aattttatat
     2701 tggttgtagt gaatgaagaa cactttttat ttgaatttgt aaaaaatatt attttgaaga
     2761 actctcaact tgaaaaaaat ccagttatta atctatttaa gataaactac ttgattctta
     2821 gagaagttat aaaaaatggt tttgagtctt tggaaaaagt tgaagagtta attcttcaaa
     2881 tagaagatga aatgatggac aacattaata agaatcatgt atctagaata agtgatgtaa
     2941 gaggattaac aagaattata gttaagaata ctagacctct tttgtatatt ggggatagaa
     3001 tagttaagga aaatataaga tatttaaaat attcaaatgt aaaaaaatat aatttagaaa
     3061 attttcaagg tatagatttt ggaattgaca aattatacaa tttcgcattg tcaacacgag
     3121 agttagcaga taaattatta gatatttatt cttccagagt aggtgagaaa acaaataact
     3181 taattacaaa attaaccttg ttaacagcca tatcagcacc acttacaatt ataactggta
     3241 tatatggaat gaactttaga tatatgccag aattaaactg gatttatgga tatccagcaa
     3301 ctttattttt tatgctatgt ataatttttg ttggaattat aatatttaaa ataaaaaaat
     3361 tattgtaaat tacagatgat gtttaattga ttttata
//
LOCUS       DAKBNN010000287         3373 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000287 DAKBNN010000000
VERSION     DAKBNN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.293"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            144..2238
                     /locus_tag="OUB02_003667"
                     /pseudo
     CDS             144..2238
                     /locus_tag="OUB02_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     repeat_region   2897..3321
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2897..2925
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
BASE COUNT         1330 a          311 c          550 g         1182 t
ORIGIN      
        1 tataatttaa aatatgaagt aataaacttt ctaattaaat taaggtattt agtgtatatg
       61 tataagttat aagtaaatct tctaaaaaga ataattttaa caatgttgtt ataaagagca
      121 gtaactatgg aggagataag tatatgaaca aacataattt tgaagttata ttaaatcaac
      181 tacagattaa tatatatgtt acaaatattc atactaatga aattattttt atgaacaaaa
      241 aaaatgaaag aagaatataa tattttagat ccagaaggaa aagtttgttg gcaagtatta
      301 tatcctgaaa aaaattctac ttgtagtttt tgtaaagttt tagaattatt aaagaatgat
      361 aaaaaaggag tattaataaa gtggtatgaa aaatgtaata agttaaatcg tgtttttgaa
      421 aattatgata gtttaattac atggcaagat ggcacagttg ttcatatgca tcaatcaata
      481 gatattgcaa actcaacaag cttaaataaa ccaattaaga ttaatgaatt tcatgaaata
      541 tcaaataata aagaagaaaa aggagttttt aatttttcaa gagacaattt tgattataat
      601 tctacattgc tttacgatgc tttgattaga ggtacagatg aatatatata catttgcaat
      661 atgaaaacag gcgtatttcg atattcacca tcacaagttg aattatttga cttaccagga
      721 gaaattgtta aaaatccgct tgtttactgg aaaaaaattg tacatccaga agactggaat
      781 cgtttttata agtcaaacac tgaaataggt aaaaaccaga tggattatca tacagtggaa
      841 tttagggcta aaaatcgtag tggagaatat atatggttaa gatgtagagg tcaactaatg
      901 cgtgatgaat ttggagagcc aagtattttt gcaggaataa tgacgcaatt aggtaaacaa
      961 aataagattg attcattgac acagttactg aattatcatg aatttatgag tgtatttgaa
     1021 gataaaattt caaatcctat gattgaaaaa ttatgcatag tattattaga tattgacgat
     1081 tttaaaaatg ttaatgagat gtatgatagg gattttggag ataatataat aaaaacacta
     1141 gcacagtctg tacaatctat tttaccagat aatgctgaac tttataaact tgatggggac
     1201 gaaatgggca ttcttgttga taatgttgaa gaaaatgaaa ttctaactct atataatcaa
     1261 atacagaata tgattataca ccttcaacta tggagaaaat atggattaaa tataacaatt
     1321 tctgctggtt gtgtaatata tccaaaacat ggagatacag taaaagaact ttataagtgt
     1381 gctagttact ctttacaata tgcgaaagag catggtaaaa acaggcttgt ttttttctcc
     1441 caagaaatat taaaaaataa aatgtattca ttagaaatga tgcgggattt aaaggcatca
     1501 attaatgatg attttagggg gtttagtctt cgattccagc cacaagtaga tacagaaagt
     1561 cataaaataa taggtgtaga agttctttta aggtggacta atgacaaatg caaagcaatt
     1621 tctcctttgg aatttattcc aatattagaa gaaaatgata tgataaatat tgtaggggct
     1681 tgggtgcttc gaatggcatt acgtactttt aggaaatgga ttgattacta tccttttttt
     1741 aaagtgagtg taaacgtttc agctgttcaa atattagaag acacatttat tgaagatata
     1801 gtaaaaatta tagatgatga aaattttcct tatcaaaatc ttgttttaga attgacagaa
     1861 agtcatacag tacaaaatat gagtatttta cagtttaaat ttaaggcttt acaagattta
     1921 ggaatttata ttgctatgga tgattttggt actggatact cgtctctaga agttttaaaa
     1981 ttttctccaa tagatatagt gaaaattgat agagtatttg ttaaagatat attaaagagt
     2041 aagtttgatg ctacatttat acattttatt gttgcaattt gtcatgatgt aggaataaaa
     2101 gtatgtttag aaggtgtaga aacacaagaa gagtatgatt tagttaaaca aataaagcca
     2161 gattacatac aaggatattt atttggaaaa ccgcagacag caacagaaat ctttgacctt
     2221 ttaaagctag ataattaatt ctaatttatc aataatatca ctgaaaagct ttaaaatcaa
     2281 aaatacatta aattattata ttgaagatat atcaattata aattgcaata taagaaaata
     2341 gcatttcaat ggatgttaaa ttgggaatag tatatgatta aagtatttta aaaagggata
     2401 accataataa aaatagatat ctatttttag attaaaaata atatatcata aataaaataa
     2461 taagaggtag atacagtttt aagggaatac aaaagttttt aattaaacta tgcttgttca
     2521 gatagatatt tatttaagaa aaaagactat taaaagcaat atacaagaat gatatattag
     2581 attgattaaa caagcataaa tattatgtaa aaaactttaa gttatagaat ttaaatctaa
     2641 tgtagataga ttacgttttt ttgcttttat tatggtataa attggtatca atattcaaaa
     2701 gtaatatatt tatgatataa taaaattata ggaattttgc agtgagcgat atttgtgaaa
     2761 aaatttggcg taacagttga aatataaggc gttgagagtg catgataagc gttatcaatt
     2821 gcactattgc tcgttcactg caaatttagg agagttgtat acgtgtaagt gttgaaaata
     2881 ctaagtttat tttggtgttt tatattaact atatggaatg taaatacggg aaagctagag
     2941 ggtgtttgga ctttagagga aagttttata ttaactatat ggaatgtaaa tcgttcttgg
     3001 aatgataaat atagaattgg ttggatagtt ttatattaac tatatggaat gtaaattttt
     3061 aagctccacc ttgtcttaaa agtttaagca atagttttat attaactata tggaatgtaa
     3121 attatactta taggagttgg aattataata ttaactacaa gttttatatt aactatatgg
     3181 aatgtaaata tttgatttta atggtacgtt ttcgctttct ctaaggtttt atattaacta
     3241 tatggaatgt aaattacaac gacttatatt ctaaaataaa aatgtaaact tcgttttata
     3301 ttaactatat ggaatgtaaa tctatctttt gttgctttac ataaatttat attacttatg
     3361 ttttatatta act
//
LOCUS       DAKBNN010000288         3365 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000288 DAKBNN010000000
VERSION     DAKBNN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.294"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(271..2190)
                     /locus_tag="OUB02_003668"
     CDS             complement(271..2190)
                     /locus_tag="OUB02_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8887386.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            complement(2227..3144)
                     /gene="pfkB"
                     /locus_tag="OUB02_003669"
     CDS             complement(2227..3144)
                     /gene="pfkB"
                     /locus_tag="OUB02_003669"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCT8887387.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKGGALLITEEEEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
BASE COUNT         1062 a          654 c          358 g         1291 t
ORIGIN      
        1 atatacaaaa aaatacaaca aatattattt tatagttcga gtgaatatta aaataatata
       61 taattttctt tatgttctaa cttgaaaaca atttaaatac aaataaagca aaacacactt
      121 attttactta tataaaaata aaagactgtc aagataacaa ttacaaatcg ttatataaca
      181 gcctcataaa atccttattt taaaataatt cagcaatata ttaattttta taaaacatca
      241 tattttaagt tcttgaatat cctttaattg ctatacttct tgatttaatg gttttttaag
      301 taatgctaaa agcatcattc caaccaaagc gcctacagct atagctaata tatatgcaaa
      361 tggatgggta accactggta taacaaatac tcctccatga ggtgcacgaa gagctgcgtt
      421 gaacaacata ctaagtgctc ctgccactgc tgaacctcca acacaagctg gtataacatg
      481 aagtgggtct ccagctgcaa aaggtatcgc tccttcagta acaaatgata atcccattac
      541 ataattaact ttaccagaat ctctttcttc tttagtaaat ctattcttaa aaaatgtagt
      601 tgcaagagct atagcaagtg gcggaaccat accaccagcc attactgctg ccattatttc
      661 aaactgccct gattcaagtg atgcaactcc aaatacatat gctgctttat ttacaggtcc
      721 acccatatcc acagccatca ttccacctaa tactaatcct aaaaatattt ttgaagttcc
      781 acctaaacca tttaaaaatc caaccattgc attattaatt gctgtaactg gaggtattac
      841 taaaaatgtc attataattc ccatcaaaag tgttccaagt aaaggatata acaatactgg
      901 ttttattcct tccaatgaag ctggtagtac actaaatatt ttcttaagcc cttctactaa
      961 ataacctgct ataaaacctg caagtagtgc acctaaaaat cctgaaccac catcacctgc
     1021 tagagcacct cctacaaatc ctacaacaaa cgctggtcta tcagatatag agtatgctat
     1081 atatccagca agtacaggta acataaatcc aaaagcttta tctcctattc ctttaaaaaa
     1141 agctgctata ggtgtatttg aaccaaagtt tgaagggtta atagtataat catcaagtaa
     1201 aaaggctatc gctataagta tccctccacc aataacaaat ggtaacatat tagatacacc
     1261 attcattaag tgtttgtata cttttctaaa tccactttca ctttcttcat ttgattcgtt
     1321 gtgacttcca tctcctccat ggtaaattgg agcatctcca tttacagcct tgtctataag
     1381 ttcttccgct ttgtgtatac catcagccac cttagttttt atgacttttt tgccattaaa
     1441 tctagccatc tcaacatttt tatctgctgc cactattata catgttgcat tttctatttc
     1501 atcttttgta agcttatttt taacacctgc tgaaccatta gtttctactt ttattgatac
     1561 acctttgttt tctgccattt tatttaaact ttcagctgcc ataaatgtat gtgctattcc
     1621 agttggacat gctgttactg ctaaaacctt aggatactta ttagcatcat tctctttagt
     1681 atctattata tttttttcag taactttctc atcttgatat tcttcactga atttttctct
     1741 ttccttttta tctattattt ccttaaattc tttttcagag cttgaattta taagtttatt
     1801 tctaaagtct tcatccataa gtattgtaga taatcttgat aatacttcaa gatgagtatt
     1861 gttagctccg tctggtgcag ctatcataaa aaatagattt gataaacttc cgtcaaacga
     1921 ttcataatct ataccttctt ttgacactgc tgctgctata caagctttag taactgaaga
     1981 atttttagca tgaggtatag ctacaccttc tccaattcct gttgtactca tttcttcacg
     2041 agccaaaatt gcttttttgt agttttcttt atcatttaaa tttccactat ttgcgaccaa
     2101 atctactaat tcttctattg cttctgattt ggtttttgaa tttaaattta acttaattga
     2161 cttttcatct aataaatcta taattttcat aaatattaat tacctttcta tatatttttt
     2221 aatattttaa aacttcttta cagatatctc atttaccaat ttatcaatgt attctctttt
     2281 ggctaagtca tatgaaaaag cagttgcact acctgaagct gctcccaatt taagagcata
     2341 atcatatttc ccagtattca aatacccaga tataaatcct gctaccattg aatcacctgc
     2401 tcctacagaa ttttttactt ttccttttgg aacatcactt ataagcactt cttcttcctc
     2461 tgtaatcaac aatgctccac cttttcccat tgataccaag acatttatag ctcccatttc
     2521 ctttaacctt ctagcatatt ttaccatgtc ttcaattgaa tttaatttaa caccaaatag
     2581 ttcttctaat tcgtcgttat taggcttaat tagaaaaggt ttgtatttta aaacattaag
     2641 aagaagattt ttagttgcat ctactacaac ttttatattc tttttttcta atctagccat
     2701 tatattttcg tatagttttt catctaaagt agatggtata cttcctgcca atattaaaat
     2761 atcatcttga tttagcttat caagtttttt atataaagta tcaatgtctt catcatctat
     2821 atttggacct tgtccattaa tctcagtttc ctttaacgcc tttattttta catttattct
     2881 tgaaaaacca ctttttatct ttataaaatc tgtcttcaat tctttttctt ccaatgttct
     2941 tattatatat tctccagtaa aaccacttat aaaacctaaa gatgtgttat catttccaag
     3001 ttccttaagt atgcgagata catttatacc ttttcctcct ggataaacgt attcttcttt
     3061 tacacgattt gtacttcctg tctttagctc atctagctta actatataat ctattgatgg
     3121 atttaatgtc actgtataaa tcatattgcc acctcttttt tatttatttg ctttatgttt
     3181 atatcttaac tttatattaa tatccttgaa agtcaaaaaa tttctcttaa ttaaaagaga
     3241 aatttatcgt ttttatctta tttagtttta tattatccaa atttacaggt gtatatacaa
     3301 tactacccat ctaattaaat aaatttacaa atttttcaaa actactattt tcacatatac
     3361 ttata
//
LOCUS       DAKBNN010000289         3345 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000289 DAKBNN010000000
VERSION     DAKBNN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.295"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            159..1052
                     /locus_tag="OUB02_003670"
     CDS             159..1052
                     /locus_tag="OUB02_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT8887388.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1230..2054
                     /locus_tag="OUB02_003671"
     CDS             1230..2054
                     /locus_tag="OUB02_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8887389.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2153..2761
                     /locus_tag="OUB02_003672"
     CDS             2153..2761
                     /locus_tag="OUB02_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8887390.1"
                     /translation="MKKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            2865..3041
                     /locus_tag="OUB02_003673"
     CDS             2865..3041
                     /locus_tag="OUB02_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887391.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         1251 a          390 c          464 g         1240 t
ORIGIN      
        1 taattatatt tagtgtaaaa ataattaatc aaaatataaa aaataggata ttattatagg
       61 caataactaa catcaagcga taacttaata catataaaaa taaataaaca attaataaaa
      121 attaaaactt cattataaaa cggaaggata gtgaaaaaat gcaactatat aacaccccct
      181 ttaatacaaa caaacactta atctatttct ttacatttac gctagtattg aactggatat
      241 tcagtcttac acctgcgttg ctcggaatta caaatacaat acttggagaa atcatttttt
      301 atcttgcata tacatcacca gctattgtag gtctattttt tatttctaca atctatccac
      361 aaagtgcaaa acaagatttt atttatagat acctaagctt taaagaaatt agaataaaat
      421 ggatactatt aacaatatta ttttttgctt taacttttat tacttcgtta ataatcggaa
      481 attactttaa tgtaaaaact tctgatatag attggataca catagcagtg tttagacctt
      541 ctaaaatagc ttatatgcta ttagcaagtt ttatattgag tatacttagt caagaagctg
      601 gatggagagg atatgccatt gacaaattat tagttagatt tggatttata ggctcttcaa
      661 ttattttagg gcttgtttgt ggaatctggt atctagggtc gtattttaca cctaatcaaa
      721 taccatataa tttggcacaa tactccctat ttgatgcttt tttatttata ccaaacattg
      781 tacttttaaa ctttataatt aattttgtat atatcaatac aagtagaagc attttatcag
      841 ctgggttagt tcatatgatg tactattttt tcaacattca attacttcta cactacccta
      901 ttaaattatg tgtaatagct caatatgtac aaataatatt tggtttaata tttcttgtat
      961 atatgatttc ttctaataaa tttaaacaga aggtaaattc tgaaattgaa cagataaagt
     1021 cggatgattt tgaatttgag ttagactggt aattttaata tgaattataa attaagataa
     1081 tctatgattt gatacgatta ctacttaaat ataatttact ctctataatt tattgtaaca
     1141 tctggaaaat aattgtattt ccacaaacgc ttgaagtata taaaaacaaa atatatactg
     1201 attataagtg aaaaggaggt catattttca tgtttaaaat aggagaggtt tcaaaactaa
     1261 cacaaatttc tattcgtatg ttgcgttatt atgatgaact tggtatttta aaacctgcaa
     1321 aaacagacaa atatacaggt catagattat actcagttga acagatttca atattacaaa
     1381 gaattgtatt attgagagat tcaaaattta gcgttgcaga aatagcaaat atcgtacata
     1441 attggaatga tgaatttgta attaaggaat tgaatagaaa aaagaatgaa atacaaaagg
     1501 aaataaagca ggaacaacaa agaataaata aaattgataa atttatagaa gctataaact
     1561 gtgataaaga tgaaattcat tacaatgttg tctttaaaaa gattccaagt tataaaatca
     1621 tatcacttag agagataatt ccagactacc aatctgaagg tatactctgg gaaaagctat
     1681 ctaaatttat caaagaagaa catatagaag tatcacgtca atccaacaat aacatagctt
     1741 tttaccatga tgaagaagtc aaagataatg gtgtagatat tgaagttgga atggttgtaa
     1801 agaagattgg aaagaacaaa tctggattta tatatcgtga gacagaagaa attgatatga
     1861 tggcatgtac tatggtttat ggtccatatg aaaatatagc tggtgcatat gaatcgttct
     1921 gttattggtt agataaaaat agtgattatc aaatatcagg tataaataga caaatcggtc
     1981 ataaaggtga acataatgaa ataaatcctg aaaattatct tacagaaatt caaatccccc
     2041 ttataaaagt ctaatcgatt agtacttaaa tacttaagta ctaatcaccc attgacatcc
     2101 caaaattttc atgatagtat ctaaacgtgt attaaacatg tttaattttt ttatgaagaa
     2161 acttttaagg ggaaaaatta tgaaagcaga ttcaacaaag gaacaattaa ttaatgccac
     2221 aatccagtta ctatcttcac atagagatgt ttcaagcatt acggctagag aaattgtagc
     2281 taaagcaaat gtcaatttag ctatgattaa ttattatttt aaatctaaag aggagttaat
     2341 taatatttct attaaaaaat ttttaaagcg tcatatggat gaatataagg ctacacatga
     2401 tactaaaaat ttagcaccta aacagcaatt aagggaaata ctaattgcat tttttgattt
     2461 tataggtgaa catagtcaat ttataaagat ttctgtaccc tatatattga tacaggaaga
     2521 aattgtttat ccatttgaga tactaccttt aattaaggct cactatgaaa atcagaataa
     2581 caaaaaaact gaagctgaat acagaactat tgcgtatcaa ataacttcat ttattcagtt
     2641 gctttttctt cgttctaatg cttttttcaa gtatagtggt attgatattc ttaatgttga
     2701 acagagaaat ggtttaatag attttcaatt agatttatta ttggtagata ttacaaaata
     2761 atactatatt aattttttat gtacattttt ttattttatg gtaacgtttg aaaatattct
     2821 cattgactaa aaatacttgc attaaataag ggggaggaat tttaatgttt tttacattta
     2881 taggagtaat atcagcaatt ttattgttgt gtacaattat atgtggttta tggataaaac
     2941 ttaataatcg taataatgac tctaattctc actttacttt ttcaatactt tcaattgctt
     3001 tttcactgtt aattgtggtg gcctttttag taagaaatta atgtctttgt tatgctgagg
     3061 tttattatta tagattttta ttattgcatg atttttacta tattatagta tttttattat
     3121 attacagggt cttatttatt atagtgtttt gttgttacag cattttatta tattacagag
     3181 tcctatttat tataggattt ttattatatt gtaaaatctt atttattata ggattttgtt
     3241 atattacaat ctcttattta ttgcagtgtt ttgttattac agtatttcta ttatattgca
     3301 aactcttatt tattatggta ttttgttatt gcaacatttc tatta
//
LOCUS       DAKBNN010000290         3326 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000290 DAKBNN010000000
VERSION     DAKBNN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.296"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1901
                     /locus_tag="OUB02_003674"
     CDS             <1..1901
                     /locus_tag="OUB02_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCT8887392.1"
                     /translation="NVFEYIKKSLPFTEEEKKWLEKHKNLIYSSDQSSPPLRYKGKED
                     GQYKGIIVDLINSLSIQIGRDFYFKPNNWWKESFVNSIDDSIKFFDLIPSKERANKFI
                     FTDPIYTLSANILKDKKSQDINSYMDLKGKTVAIPEGDYSINFLKQKIQDINILLTPD
                     IKTGVNHLMSGKVDAVVGDEPVLRYYINNYGLSNKYSVLSNPIYTKKAVLAVPKQYEE
                     LVSILNKGIFKLQKNGVYKDLKKKWYSTYNEVDDILYERGIVPSIYLFIGIILISIYV
                     FYSYTYLLKIEIKRKTEQVIENKKTLEATFNSITDIIMLVDENNNIVESNKVLYDFMG
                     EMSYKIADLISMIKGVIENTFSENTNKTSEIEIHNKILKINTFPVEYKKNNTEYIVVL
                     IKDITNDKIVEAKLLRENKMISIGQLASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVN
                     DCVKSIESNVDRASNIITNLLNFARISDDNLEHINIRNFIENIVKLQYKMLQLKNVEI
                     KIDCEHNLICYINGESLKHVFINLISNSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGE
                     GIKEDALKDIFNPFYTTKPIGEGTGLGLYITYNEIKKNNGDISVESKLGVGTCFHIKI
                     PLNKEVTI"
     gene            1901..3223
                     /locus_tag="OUB02_003675"
     CDS             1901..3223
                     /locus_tag="OUB02_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HCT8887393.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         1419 a          294 c          558 g         1055 t
ORIGIN      
        1 taaatgtatt tgaatatatt aagaagtcac ttccatttac agaagaagaa aaaaagtggt
       61 tagaaaaaca caagaatttg atttacagct ctgaccaaag ttcacctcct ttgagatata
      121 aaggtaagga agatggacag tataagggaa ttattgttga tttaataaat tctctgtcaa
      181 ttcaaattgg tagagatttt tattttaaac caaataattg gtggaaagaa tctttcgtaa
      241 attcaataga tgatagtata aagttttttg acttgattcc atcaaaagaa agggcaaata
      301 aatttatatt tactgaccca atatacactt tgagtgcaaa tatattgaaa gataaaaagt
      361 cccaggatat aaatagttac atggatttaa aggggaaaac agtagcaatt ccagaaggtg
      421 attattctat aaatttttta aagcaaaaaa ttcaagatat aaacatttta ttgactccag
      481 atataaagac tggagtcaac catttaatgt ctggaaaagt agacgcagtt gttggtgatg
      541 aaccagtatt gcgatactat ataaataatt atggactttc aaataaatac agtgttttaa
      601 gtaatcctat atatactaaa aaagcagttc ttgctgtacc taaacaatat gaagagctgg
      661 ttagtatttt aaataaaggc atttttaaac ttcagaaaaa tggagtgtat aaagatttaa
      721 agaaaaaatg gtattctacg tataatgaag ttgatgatat attgtatgag cgaggaattg
      781 tgcctagtat ttatcttttt ataggtataa ttttaatatc aatttatgtt ttttatagct
      841 atacatattt gttaaagata gaaatcaaaa ggaaaacaga acaggtaata gaaaataaaa
      901 aaactttaga ggcaactttt aatagtatta cagatataat aatgcttgta gatgaaaata
      961 acaatatagt agaatcgaac aaagtactat atgactttat gggtgaaatg agttataaga
     1021 tagcagattt gattagcatg ataaaaggtg ttattgaaaa taccttctct gaaaatacta
     1081 ataaaactag tgaaatagaa atacacaata aaattttaaa gataaacact tttcctgtag
     1141 agtataaaaa aaataatact gagtatatag tagttcttat taaagatata actaatgata
     1201 aaatagtaga ggcgaaatta cttagagaaa acaaaatgat atctatagga caattagctt
     1261 ctggggtagc acatgagata agaaatcctt tgggtataat aagaaataat tgctatctct
     1321 taaaagataa cgttacaatg gaagaagtaa atgattgtgt taaatcaata gaaagtaatg
     1381 tagatagagc aagtaatata ataaccaact tgttaaattt cgcaagaata tcagatgata
     1441 atttagaaca tataaatata agaaatttta ttgaaaatat agtgaaactt caatataaaa
     1501 tgttacagtt aaagaatgtt gaaataaaaa ttgactgtga acataattta atatgctata
     1561 taaatggaga atcgttaaaa catgtattta taaatttaat ttctaattca atagatgcaa
     1621 tacatcaaga tggaaaaatt attatatact gttatgaaaa aaatcattgt ttatttatag
     1681 attttaaaga taatggtgaa ggaataaaag aagatgccct aaaggacata tttaatccct
     1741 tctatacaac aaaaccaatt ggtgagggaa ctggtttagg tttatacatt acttataatg
     1801 agattaaaaa gaataatgga gatataagtg ttgaaagtaa attgggggtt ggaacttgtt
     1861 ttcatataaa aattccatta aataaagagg tgactatata atgaaaatac tattagttga
     1921 tgatgagcta gaatatggag tggtaatgaa aaaaattcta caaaagaagg gctatcttgt
     1981 agatatagca ctaagtggtg aagaagctat aaatataatt aaaaaagata aaaattatga
     2041 tttggtttta tctgatgtta tgatgaaaaa tatggatgga gtacagctat tagatagaat
     2101 taaatctatt aatagagata tagaggttat attagttaca ggatatggaa gtattgaaaa
     2161 tgcagttgat gctatgaaaa gaggagcttt atcttatttt ataaaaagta atcccattga
     2221 aaatttatta gaagaagttg aaaaggtaaa ggcaagtaaa atatctgttt ctgtacaaaa
     2281 aaataatttg gaatttactt tggaatctaa aaatagagat tttaataata tcataaaaat
     2341 agcaaaaaaa gctgcttgta aagatgttaa tatattaatc ttgggagaat ctggtgtagg
     2401 aaaagatata ttagctagat atatacatag cataagtcca agaaagaatg agatatttgt
     2461 acctgtaaat tgttgttctt tttcagaaaa tctattggag tctgagttgt ttggtcatga
     2521 aaaaggttct tttacaggag cagtagatag tagaaaaggt agatttgaac tttcgaataa
     2581 aggaactcta tttttggatg aaataggaga tatacctcta aatgttcagg taaaactttt
     2641 gaggacactt gaggacaaat ctatagagag gataggaagt aataaatcta ttaaagttga
     2701 ctttaggtta atttgtgcta tgaataaaga accaaaagtg gaaatatcaa atggaaatat
     2761 aagagaagat tttttttata gaataagtac aataacaata actataccac cgctaagaaa
     2821 gagaagagaa gacttaacta ctcttataga atttttctta aataagtacc aaattgaaca
     2881 tgataaaaag atacactcga ttgataagga agtaaaggat tttttattaa attacaatta
     2941 tccaggaaat ataagagaat taaaaaatat aataaataga cttgtagttt taagtgaaga
     3001 aggcaattta tcaaaagaca atttaaattt aatatcaaat aatgtataca tagatgataa
     3061 aataagtatt agacctttaa gagaaataag aaaagagttt gaatgtgagt atattgaaaa
     3121 agttttatct ttgtgtggaa ataatatttc aaatacagct aaaaaattgg agattagtag
     3181 aagacagtta accaataaaa tatcagagta taatattaag tgagaaaaaa acttctcatt
     3241 aagtgagaaa aaaatttctc aaactgtatt aaaagataaa cagaaaaaat ctgtttatct
     3301 ttttttatta taattttaaa aattaa
//
LOCUS       DAKBNN010000291         3272 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000291 DAKBNN010000000
VERSION     DAKBNN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.297"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            33..884
                     /locus_tag="OUB02_003676"
     CDS             33..884
                     /locus_tag="OUB02_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HCT8887394.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1017..2630)
                     /gene="ggt"
                     /locus_tag="OUB02_003677"
     CDS             complement(1017..2630)
                     /gene="ggt"
                     /locus_tag="OUB02_003677"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCT8887395.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGSVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2855..>3272)
                     /locus_tag="OUB02_003678"
     CDS             complement(2855..>3272)
                     /locus_tag="OUB02_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887396.1"
                     /translation="PFNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQ
                     LSNIYDDKERYIMLIKLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFI
                     PRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
BASE COUNT         1081 a          532 c          434 g         1225 t
ORIGIN      
        1 taaagataat ataaacacag gaggtacatt ctttgaaaaa cattatttca gcaaaagaac
       61 ttatatcaaa actagaaaaa aatgataatc tagttgtaat agattgcaga tttgatctta
      121 tcaatagaac ttatggtatt gattcttata aaaaaggaca catcaaaggc agttttatct
      181 tagatataga caaggattta tcatctccta caaaagaaca tggtggtaaa aatcctttac
      241 aagacccttt aatactaaaa gaaaaattag aaaaaatggg tgttgataat gacactacga
      301 ttgtaactta tgatgatgga gatttaaatg gagcttgcag attatttttc caattaaaac
      361 atttggggct taaaaatgtc tatgtactag atgggggaat aacatctttc gtaaaggaag
      421 gtggagaatt ggaagagaaa gtgaatatac caagttgtac aggtaaggaa attagaccaa
      481 atataaacaa tggtttagtt gttccaatgg aatatgttaa gtctaaatta tacaaaaaag
      541 atactgtaat aatagattgc cgtgctaatg agagatacca aggtttagtt gaacctgctt
      601 actcaaaagc tggtcatata ccaagtgcaa aaaattattt ttgtaaggat ttaataaaaa
      661 gtgactttga aaatggctca cttaaagata tagaattttt aaaaaaattc tttaaagatt
      721 taaataatta tgatgaagta attttatctt gtggttcagg gatatctgct tgtgttaaca
      781 gtcttgctct tagagaactg gatatacctc acaagattta tattggtagc tttagcgact
      841 ggattagtta tgatgacaat gaaatcaaaa ctggtcaaga ataaggctca taatctatat
      901 aaaatcagca tatgatagct taaattttgt aaaggcgtca gtcattgtag actttcgcct
      961 ttacttatta tttcatatac tctttaaatt tattatatat attttagttg ataatattaa
     1021 taaactgcaa tatgaccatc acatcttgat tccgtcccac aaatataact ttcctgttct
     1081 gttttaaata ttatttgacc tcgtcccatg tcaacagtat catgaattac ttttatttca
     1141 tgaccctttt caattaatga atctattata tgctttggca tctctggttc aacttctatt
     1201 tcttttcctt ttatccactg ccatcttgga gcatctagcg cctcttgtgg atttaatcca
     1261 aaatcaatca tatttgttaa cacctgaata tgtccttgtg gttgcataaa agctcccatt
     1321 acaccaaatg gacctattgc tttatcttct ttacctaaaa atccaggtat tatagtatga
     1381 taaggttttt taaatggttt tactacattg tggtgtttag ggtctaaatt aaaattattt
     1441 cctctactat gtaaagcaat cccagttctt ggtatcacaa ttcctgaacc aaaatttatg
     1501 taattgctct gtatatatga aaccatattt ccatccttat ctgcagtaca aaggtatact
     1561 gtaccaccac aatatggctc tcctgcagtt ggatacaaag cctctttatt gtcaattaat
     1621 aaacttcttt ttttggcata ctcttggctt agtaattcct gaatctttac cttcatatgc
     1681 tctatatcag ttacatatgt tttagaatct gcaaaagcta attttaaact ctctataatt
     1741 ttatgtatgt cttctacatt ttctatattt ccttctaatt ccaattcttt taatatgtta
     1801 agtgccatta aaactgttat tccatgacca tttggtggga tttcaaatat tttatatccc
     1861 ttatattctg tacttatagg ttctacccat gttggataaa aacactctaa gtcactcttt
     1921 cttatagccc cattaaattt tttagaatat tcatctattt tatcagcaag tcttcctcta
     1981 taaaagcttt cagcctgagt atttgctatc tcttctaatg tacttgcctg ttcttcacat
     2041 ataaatatat ctccagcctc tggagcttta ccatctttag aaaatgtatc aaaccatggt
     2101 ttgaactctt ctccaataag ttctttttca tagagttcat atgatttttt ccatacctta
     2161 gctacattag gagatactac gtatccctct cttgcatagt ttatcgctgg agataagcat
     2221 tccattaagc taagtttacc atatttttta tttaattctg accaagctgc gggtattcca
     2281 ggtacagtta ctgatccaaa cccatatttt ggcatttctt tcatattatt ataatttttt
     2341 aattccatat tttctggagc aaagccacta gaatttaaac catacaatcg tttttcttcc
     2401 tctatccaaa caagggcata tgcatctcct ccaatcccat tgcttgtagg ttctactact
     2461 gctaatgtgg cggctgttgc aactgctgca tcaacagcat ttccaccctt ttttagtatt
     2521 tcaagtccag cttgagatgc taatggtgtt gatgttgcaa ctgctccatt tttagcatac
     2581 acgacatttc ttattgatga gtatttatgt tggtaggcat taaacttcat aaatttcagc
     2641 tcctcgtcat tttttatttg atattctgct tagatttatt atataacatc aagtaatgta
     2701 actgatacaa aatgatataa aatatagact ttttcctaac tttaatttta tctttaaata
     2761 aatatcttaa actcaattct agtattttct atctgcactt aaataagtta ttttattcaa
     2821 aacatatatc taattctttt gacttaaaaa taatttattc ttttatcatg tttagagcat
     2881 attttaaagt taaaaaataa tatataaaat atcctataaa ataaatgact agagttatga
     2941 taattggtac aagtaatgac cttggtataa acttttgtgc tatagacata gcaaataaat
     3001 tatgaactgt acctacaact aatggcatta aaaatataac ttttaactgt tttgatatta
     3061 ttttctcaat atctttatta tttgctccaa gttttattaa cataatatat ctttccttat
     3121 catcatatat atttgataat tgtttaaata agattatact acctgtacat aaaagaaata
     3181 ctacagacaa aaatatgcct ataaacagca ttagaccact taattttatc aagttattat
     3241 aactttcaat acttgatgta aaattaaaag ga
//
LOCUS       DAKBNN010000292         3257 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000292 DAKBNN010000000
VERSION     DAKBNN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.298"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            197..844
                     /locus_tag="OUB02_003679"
     CDS             197..844
                     /locus_tag="OUB02_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HCT8887397.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1011..1580)
                     /locus_tag="OUB02_003680"
     CDS             complement(1011..1580)
                     /locus_tag="OUB02_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8887398.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1585..2484)
                     /locus_tag="OUB02_003681"
     CDS             complement(1585..2484)
                     /locus_tag="OUB02_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCT8887399.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2495..2644)
                     /locus_tag="OUB02_003682"
     CDS             complement(2495..2644)
                     /locus_tag="OUB02_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887400.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2637..3221)
                     /locus_tag="OUB02_003683"
     CDS             complement(2637..3221)
                     /locus_tag="OUB02_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCT8887401.1"
                     /translation="MKRFFIVVLGCIFVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1136 a          556 c          366 g         1199 t
ORIGIN      
        1 ttattcagtt atttattaaa aactaatagt aaatcttata ctaatcgcat atttatgtta
       61 taactatatt gtaactattt tcgtaatctt aggaaaaggt tacttataaa attaacctta
      121 tacaataata tatcgtataa tcttaattag cataatagtt acaatattaa aatttaaata
      181 tgggagtgat tttagtatga taagacaaac accattagag aaacgatatg acaagctagg
      241 tcctcatatt gtaacagcac tcaaaaaacg ttattttgat gcttattact gtaaaactag
      301 aaatgaagct ttgcaacaaa ttttagattt aattcctcaa aatcatgtag tatcatgggg
      361 aggatctgaa acacttaaag aaatgggcgt acaaactgct gttagagaga aaggatttaa
      421 agttattgat agagatttag caaaaactcc agaagaaaga actgaaatta tgcgtcaagc
      481 acttctatgc gatacttttt tgatgagtag caatgcaatt agtgaagatg gacaactctt
      541 taatattgat ggaaatggca atcgtgtagc tgcaatgata tttggtccaa aaaatgttat
      601 agttgtagct ggtatgaata aaattgtaaa aaacattgat gatgcaatac aaagaactag
      661 aacaattgca tctccagcag ttgttcagcg ttttcaagat gtcaatacac catgctatat
      721 taatggaagt tgtatagatt gcacaagtcc tgaaagcatt tgtgcataca tggttactac
      781 aagaatcagt cgtccagcaa aaaaaataaa ggtaatattg gtaggagaaa atcttggtct
      841 ataaaatata catacatgta aatagctcta taaataataa aatcatatga aataacttat
      901 ttctaaaagt ggtagaataa aagcttctac aagactttca ttctaccact tttatattct
      961 tttaatttgt gtttctatat tattaattgc tccgattttc atatatatta ctaactattt
     1021 ttattttcat aatttttttg ttcttcatca agctgtttat aataatcttt attatagtca
     1081 gctagataat ctataccacc tcctgtaatc acatttaata atttcattgc agattcttga
     1141 ttaactttac aaacataatt atcaccagat ttaacaagct ctaatgtaat attaaattct
     1201 tttttctttg cagaatcaat atctttcata gttggatttg gcatcatttc tcctagttta
     1261 ggaggattct tttttaaaat agaaaccata tcagtattgt taatttttaa atctacagtc
     1321 actttatcct tattattctt tatatctgaa atttcccaag tcatattttt agaatacatt
     1381 ttaaatcctt ctttatcttc gtatataaga ttacctaacc ctgattttgg gaaatactta
     1441 tcaacagtat ctgcatcttg tgtttttatt gaattaaaca tactatccac ttcatttttt
     1501 atcttattat cattagtaca accagctaac accagtactg atattgcaaa cattataata
     1561 catataataa atttcttcat taaattatat ccccctatac ttttttgcac aaatattatt
     1621 ctctttttta tcttttgaaa aagttgtagt aattgcattt tgtggacaaa cttttataca
     1681 ttcaccacac ctaatacagt ctggactatt tacatcttta tatattggta tatcaagctt
     1741 acattttgtt tgacatgctg tacaatttgt acatttatct ttatcaattt taaatctata
     1801 tattgaaata ggattaaaaa aaccatagat tgctcctaaa gggcatatat acttgcaaaa
     1861 tggcctataa gtaatgattg acatcaataa tatcaatagc aaaatactta acttccagct
     1921 aaataaaaat cctattgatt ctcttaaatt ctcatttcct aaaataagtg gtattcctcc
     1981 caaaagtgtt cctgaagggc aaatccattt acaaaaccaa ggatttccac cccctgctac
     2041 atttacaata gcaactggca aaataattac aaacattatt aagactatat actttagata
     2101 ttttagatat ttatcaccat aaactttttt tatctttact ggaaagggta ctttatgtag
     2161 taaatcttgt acaagtccaa agggacaaag ccaaccacaa acaaatctgc caaaaaaagc
     2221 accaaatgcc attaaaaaac caacaacata aaatgagaat ttatactctc tgcttgataa
     2281 aactgcttgt aatgaaccta ttggacatgc tccaagtgca cctggacatg aataacaatt
     2341 caatccaggt acacataatt ttttactttc tcctccatat atagttcctt ctaaaaatcc
     2401 atttacatat ccatttgtta atgctgtaaa tgcaacttga gtaataagtc ttatattatt
     2461 atttacttta gataataact tcattcctct tctcctaacc aataccaata cactcaagac
     2521 aaatcataat tgctttagtt aatactgtat ttgtttctcc acgatatgta cctaatgcaa
     2581 taaatataat actaattgca ataatagaaa tgcctttttt attatcttta ataaaattac
     2641 tcattggctt ttaccatttt tagtctttca tctataattt ttttccaatc ttccttacta
     2701 tttgcaccta caaccttctc acccacaaag tttcctttct tgtctacaaa ataagttgtt
     2761 ggaaactcca taactctttt aaaatcagtt tttttaagtt cttcagatac agtcaattgt
     2821 tggtaagttg catctgactt tttcataatg tctttaacta aatttttctc ttcatttgaa
     2881 agaccagttc tcatatcagt tttatcaact tctacaacta atccctttat agctacattg
     2941 gaattattac tctcatattc ttttgataat gcttcaagct ctggcatttc acccacacat
     3001 ggtccacacc aagtagtcca gacattaata agagtaagtt ctttatttga aaatatctct
     3061 tttgttacct tatttccatc aacatcctcc gtatctaaat tactaaacca ttcactatta
     3121 ttcttgtttt tttctactgg cttagattct tttttgctac atccagaaat catacctaaa
     3181 acaaaaatac atcctaaaac tactatgaaa aaccttttca tatctcacac tcctagaatt
     3241 tatatagtat ataaaat
//
LOCUS       DAKBNN010000293         3233 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000293 DAKBNN010000000
VERSION     DAKBNN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.299"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1086
                     /locus_tag="OUB02_003684"
     CDS             <1..1086
                     /locus_tag="OUB02_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887402.1"
                     /translation="DSNELDHININFGYALNGYINSYYTINGSNKTKKTELIKLQKDS
                     KNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYE
                     KLDMNNFTRTRYKVDKSTDDYYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTID
                     IIKINPENKKIKTIKSINLSNYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGD
                     NSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELN
                     LLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYII
                     NKNSKEIVYEGELQFNSSNSMLEFEIK"
     gene            1095..1907
                     /locus_tag="OUB02_003685"
     CDS             1095..1907
                     /locus_tag="OUB02_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887403.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1918..3186
                     /locus_tag="OUB02_003686"
     CDS             1918..3186
                     /locus_tag="OUB02_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887404.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPDISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGVVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         1387 a          252 c          494 g         1100 t
ORIGIN      
        1 gactctaatg aattagacca tataaacata aattttggat atgctttgaa tggatatata
       61 aattcatatt atacaataaa tggaagtaac aaaaccaaaa agactgaatt aataaaacta
      121 caaaaggata gtaagaacac aaataattta attgatgtat ctagtggttc agatttaatt
      181 gctcatgttg gatttgaaat gaatgaggag tcaaaagata taaactttgt gatagatgag
      241 aaaaaagaag atttagaaaa aggtgtattt ccatcagaag gtagtgataa agaaattgaa
      301 tatgaaaaat tagatatgaa taattttaca aggacaagat ataaagtaga taaaagtaca
      361 gatgattatt atgcaaagat actagcatca aaaagatata aaaaatactt atatgtatct
      421 gtactttatg ctacaagtga ttcatcagaa aataacaaag aatattcaag tactatagat
      481 ataataaaaa ttaatccaga aaataaaaaa atcaagacta taaaaagtat aaatttaagt
      541 aattattttt ccgaaaaata ccaaaacata gagaacatag gtgtaagtta tgctagcaaa
      601 tatgaggata agatgtatat tttagtaaat gtactaaata caaacatagg tgataatagt
      661 aattctaaat ttcatttact tgagtataat cttgaagaaa ataaggaaag cataatggaa
      721 ttaaaaactg atagcaaaat ggctttagca aaagcaaagt tagaagctaa taaattatat
      781 atggtaatgt cgaatgatta tgataatagt tcaattacag agttaaattt acttacctat
      841 aatatattag ataaaagctt agatgaagat aaatatgttt tagataatag agaaaaaaga
      901 ggaattagaa gtattgaaat tgattcaaat aaaatcgttg catatacatc aaggctaaac
      961 tctatattat cttgggatga taaatttatg ctatatataa taaataaaaa ttctaaggaa
     1021 attgtatatg aaggagaatt acaatttaac agttcaaata gtatgttaga atttgaaata
     1081 aagtaggtga gttgatgaaa gatttaataa gattattcga atttgaatta aaaagaaata
     1141 taaagaatta tatatttata ataatttgtt gttgttcttt tgtaatttta aacatagtaa
     1201 aaaatttaaa tgactataat tacataatag aaaatgcagt taaaagtgaa cagttaacaa
     1261 aaatcggaaa tacgatagaa acggtaaatg ttacaggttt ctcaagcttt agaaatataa
     1321 tgggttctac agaatcgtgg tttatgtttg gtataattgc atgtatttgc tatgcttttt
     1381 ttatttggta tagagacttt aatggtagaa gtaaatctat atatacactt ataatgctac
     1441 ctaaaaatag aatcaatatt tatatatcta aattattaaa tatattattt ctagtgtata
     1501 gctatacagt agtacttaca atatctttgt ttattgcaag taagttgttg cctagacata
     1561 tgcttggtaa tgtcacaaac tatggttttg tacaagaaac aatatatgag ttaaaaatgt
     1621 tacttccata tagctttgaa atactatttg tagagtatat atttttattg ataggatttg
     1681 tatctgtagt atttacatca attcttataa ataaatctat ttataaagta agcactttaa
     1741 taagttttct atttttagtt atagaaattt tagtgtttat aatttctata gaatttatta
     1801 taccttacga atacagtgat gtatttactg ttctatattc aagtataaac atatttgtat
     1861 gtagtcttat atctaataaa cttttgaagc aaaagattga cttttaggag ggatattatg
     1921 aaagcaaaaa atataggaaa atatacaata tgttttataa tagtatgttt tttaatgatt
     1981 actatgacct taatattgag ttattttaat aaggataaac tatctgtaaa aactttaaaa
     2041 ggcaatgtaa aagatatagg gagagtaagt gtagtatata cacctgatat aagcaaattt
     2101 tcttatgagg aggtaattat aagtaaaaat ggagtaagaa ataatacaaa cccaaaatca
     2161 agaattagaa atgattattt aaatattgac aaaaaataca gtgactttgt taatgataaa
     2221 ggaaaagaat ttatatataa agatgatttg aatataggag aagcgtatac ctctgttaag
     2281 tctgatttag tacaaggaat gaatgcttta aatgattgta taaaggtttc aaataaaaat
     2341 ttaaaaagtg gggtagaaga aaattttgat attaatttaa ataagtctga ttttaaagaa
     2401 aatagttgga aaaccttatt taacaaaata tataaaggta atttatatat ggtaattagt
     2461 gaaactaata tttcaggttc taaagtcaat aatatttgta tattaaagat agatataaaa
     2521 aataagtctt ataaattatt aaatagattt aatttaggat tacagaatgg agaacaacaa
     2581 aatatcaatg aaattaaatt ttctattgga agtaaattat acttagaaat agtaaataat
     2641 aaaagtaaaa atggatatat tgaaggacat gatagttact ctaactttat tatatatgat
     2701 atagaagata acacttttaa tataagtaat aaatttatgg ataatctaaa caaagaaaag
     2761 aatgcaaatc caaaggagat tggcttatta agtaaaacta agctagatta tatggtaaaa
     2821 aataataaat tgaatcttat agttacggat atgagtaatt ccataactaa tcttgtttat
     2881 gatattaatt ctaatgagat taaattagaa gattatgaga tacttgattt aaaagtagat
     2941 tataatgaag catcatctgg agttaggaaa gttaagctac tggatgataa gatatattct
     3001 atttcaggta ttaagaacag tatatcagga gaattaaaag gatttaaagg ggttgttgat
     3061 gtagtcggaa ctaagccagt aaaatttcaa gtatttgata tgaaacttaa aaagaatatt
     3121 tatgaagctg agctaataaa tggagatata agtttacaag aaaagttatt ttttgttgca
     3181 aattaagtgt tagaattaat tttagaactt taaactttta tatgtatcac aga
//
LOCUS       DAKBNN010000294         3217 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000294 DAKBNN010000000
VERSION     DAKBNN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.300"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            692..1348
                     /locus_tag="OUB02_003687"
     CDS             692..1348
                     /locus_tag="OUB02_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887405.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDDM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1856..3127)
                     /gene="sleC"
                     /locus_tag="OUB02_003688"
     CDS             complement(1856..3127)
                     /gene="sleC"
                     /locus_tag="OUB02_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HCT8887406.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
BASE COUNT         1170 a          361 c          578 g         1108 t
ORIGIN      
        1 ggggagagta tttgtagtta taaagcatat tgggaataat gaaaaaggtt aaaaacatat
       61 tactataaag tatagggggt aggttaaaag agtaatcata aaataaatat acagataata
      121 gtagataaga ataccagaaa aaaatagata gttaaagggt tagggtaaaa gagtaattgt
      181 aaaataaata tatgggtgat ggtggataag aatatcagaa aaaatagata gttaaagggt
      241 tagagtgaaa gagtaatcgt aaaataaata tatggatgat gtggataaga atagtaagaa
      301 aaaatagata gttaaagggt tagggtgaaa gagtaatcgt aaaataaata tatggatgat
      361 ggtagataag aatatcagaa aaaatagata attaaagggt tagggtgaaa gagtaatcgt
      421 aaaataaata tatggatgat ggtagataag aatatcagaa aaaaatagat aattaaaggg
      481 ttagggtgaa agagtaatcg taaaataaat atatagataa tggtggatag agtagtaaga
      541 aaataaatag ataaagggat agaataaatt taaatatgga aaatgaatat ctaataataa
      601 gtgattaata aataattgta gatagattgt aaattaaata aatctttaat agaaaggaga
      661 actatttata tattaaatag tatttataaa tatgaaaaaa ggcgatttta tatggagtgg
      721 agttcttatt tgtattattg ctgtcttagc aataccaagt agtagagaag tatttatgga
      781 agcaacaggt gcacatccat atattggagg ttttttaaag tttgctgtat tagctacaat
      841 gggagattta cttggtgcta gaattttgta taaggattgg aaaataccag taggagtatt
      901 ttatagagca atagtttggg gaataatagg tgtagttacc acatatggaa tgtcaatcta
      961 ctcatcaggt gtaggtcaag cacaagtgga tgatatgctt ccatttgagg gaatagcttt
     1021 tgcaaatgct ctatttaaga gtgcaactat gaatatacta atgtcaccaa ctgtattttt
     1081 attccataag gttatggact gttttataga taaaaaatat gaggttggaa aggggattaa
     1141 ggtaaaactt aaagatgtgg taaatattgt ggactggaat gcttaccttg gttttagtgt
     1201 tttaaaaact ataccatttt tctggatacc atgtcataca attgtattct tattccctgc
     1261 tgagtataga gtaattgctt ctgcatttgc atctatagct ttaggtctaa tattggcttt
     1321 agctaataag tctaaagttg cagtttaggt tcactgatga agatagtagt attatttaaa
     1381 ttaggaataa ctttttaaat taatattatt tgtatttata ataaataata atttatataa
     1441 gaagtaatat tctaagtgat ggatagtgtt ttaaataata agaagtaata ttctaagtga
     1501 tagatagtgt tttaaataat aaggagtaat actctaagtg agaagtagca ttttaaataa
     1561 taagtgagat tttaaataaa aagtaggttt ttcgttagaa tagcatttta ttaaacttaa
     1621 aaataaatat aatattttaa agaaatctaa gttgctaagc actgttttta agaagcttaa
     1681 aatcccccat ttaaagtttt aagattctaa aaaaacaagt tataactaaa aaatggctgt
     1741 taagactaca atacttataa gtaatactta tggtatatat tagttcataa cagccatttt
     1801 tattttaaat taataatttt attcaataat ttgagtttat gttctaaatc aagctttaaa
     1861 ttaaaggatt taaagaagct attctagttg tagctacata aactctggat atttcatacc
     1921 atgttttaaa atctactact ccgctttgag gtaaaccaaa gattccttgg aatatcttaa
     1981 cagcgttttc tgtatctgtt ccatagatac catcttctat aacttttggt acagctggat
     2041 aagaattaga tatagcattt aactgatttt gtatggttct aacatactgc ccagaagaac
     2101 caacttgtag tgtagtacca gggaatgaca ctggtacacc acttactata ggtgctcttt
     2161 caaatactat ttcatctcca tagaaatatc ttagtatact ctcatagtca tatccttggt
     2221 cacctaagtc tttactgccc cattgagtca tttggtcagg acattgggat ttttgtccat
     2281 cacaatattg tgctaatagt ggctgtcttg atgtaggagg tctttttata aaagtattaa
     2341 aaatagcatc tacaactacg ttaattggtt caaataagtt tctattattt ataaacttat
     2401 ggtcgtatgc tgttgttgaa gttattgtaa agttatatcc tttatttcta taccattcag
     2461 taaagactct atttaaagta aatgatatta tagctattac atttgcataa attgtctgtt
     2521 caggccaagt tgaatatatt tcagatgacg ctatgttttt aatgtactct ttaaatggaa
     2581 tccaatagtt tggagctgat gaatcttctg gcaaaccatc atgaactact ataaattcag
     2641 gcactacagg gttatctaat acaacgaaac cagtaggagg aggtaatggt tttaagttac
     2701 tttctggaat cttaggagga taagtaccat atagagtatg ttctcctata tcaaaaatca
     2761 actcactttg tcttgagaaa gaacgcttac ttcttgttct aggagacatg ggaactcctt
     2821 gtcttgcttc aattgtagct aacaactgtg ttccttctat gacaactgtc tcatatccat
     2881 ctgctatggc ttccactata tactggctat aaggtctaac atcagatggt tgtagagagt
     2941 aatctacatc tggtgctggt aatactaaac cagtcacttg accagattca ttacttctta
     3001 ggttttggta taaagttgat gagctttggc taccattaga cattgaatat atgtttacta
     3061 ctgcattttg aataggtcta ttgttagtag catcaattat gcttactgtt aagaaaccat
     3121 cttgcatcaa atcacccttt ctttaaatga atttagttcc cttatatttc aagatatgaa
     3181 tatagcttga aatttgttaa ataatataga tataaat
//
LOCUS       DAKBNN010000295         3179 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000295 DAKBNN010000000
VERSION     DAKBNN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.301"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            17..811
                     /locus_tag="OUB02_003689"
     CDS             17..811
                     /locus_tag="OUB02_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887407.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIELARKEDI"
     gene            812..2871
                     /locus_tag="OUB02_003690"
                     /pseudo
     CDS             812..2871
                     /locus_tag="OUB02_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
BASE COUNT         1221 a          318 c          511 g         1129 t
ORIGIN      
        1 aggagtggtt tgataaatga ttgaggtaga taaattgtgt aagtctttta caagggttgt
       61 aaaagatgat aagaataaaa gttcaataag aaagctaaag aaagtaaaaa ctaaaaaaga
      121 agaatttttt gctgtaaata atgtttcatt taaagttatg gaaggtgaaa ttgttggaat
      181 attaggtcca aatggtgcag gaaagactac attacttaga atgttaggtg gcatattaac
      241 accaacatca ggaagtataa atatttctgg atatgattat tcaatagata gaaattcagc
      301 aaaaaaagaa ataggttatc tctctggaaa tacaaaacta tatggaaggc tttctccgag
      361 agaacttcta acaacttttg caagtctata tgagatgtca aaagaagata tagaagaatc
      421 aattgaaaat attgtaaaaa taatggatat gagtgaattt atagataata gaattgaaaa
      481 tctatcaact ggtcagactc aaagaacatc tatagctcgt tgtttaatac attctccaaa
      541 agtatatatt tttgatgagc caacactagg tctggatgtt ttaagtagtg catctattat
      601 agattttatg aagaatgaaa aactaagggg aaaaactgtt ctttactcaa ctcattatat
      661 ggaagaagca gaaactttat gtgacaagat ttttatgatt cacaatggga aaataattgc
      721 atcaggaaca ccacagagtt taaaagagga atctggagta aataatttga gagatgtatt
      781 catagaactt gccagaaagg aggacattta aatgagaagt aagattgtaa aatacatatt
      841 taaaaaagaa atgttagaca tattgagaga taaaaagact ttatttatga tgattgttgt
      901 gccacttctt ttatatcctt tacttatgtt attaatgatt caagtaatga atatgtctgc
      961 aagtagtatg aatagtaagg acattaatat agcttttaat aataaaccta ataaggtttt
     1021 agtatccatg attgaagatg ataatttaga aaaagatact aaaaatactt caaatgataa
     1081 aggcaaaata agagtgttag atgtagataa ctataaaaag tcattagaag aaaataaaat
     1141 tgatgcttat ataaagatgg aagaaaaaaa tcatctataa attatcaaat atatataaat
     1201 tcttctcaag aaaattcttc taatgcacta aaaagaattg aaactgtttt ggataaatat
     1261 aaaagattta ccatagaaaa aactttatct gaaaaaggat tagatgtaca agctacttta
     1321 gaaccaataa catatagcac tgtagatgtt gcaaagacgg aagaagtggc aggatacttt
     1381 ttaggtcaga tacttccatt tatattaata ataggagttt tactaggttc aatatatcca
     1441 gcaatagatg ttatggctgg agaaaaggaa agaggtacat tagaaacatt atttacactc
     1501 ccaatatcaa acttagaact ggttatggga aaatatatgg cagtttctct tagtgcaata
     1561 gtaacagcaa ttttaaacat tttatctatg ggactgacta tggcatttat tttagttagt
     1621 ggaggtatta gtagccaatt tggttttgga aactttaatt atggagattt agtcttacca
     1681 attataacta ctttaatttg catatgtctt ttttcaatgg tagtttcagc aataagtatg
     1741 tgtgtatgtt cattagcaaa aagttttaaa gatgctcaaa actacataac acctcttatg
     1801 ttaattatta tgataccatc atatgtttct atgataccaa atatagtatt agatagagta
     1861 acagctgtaa tacctgtagt aaatatatct ttacttataa aaagtgtatt atcttttaaa
     1921 agcgatttaa atttaatagc cttagtttta gcatcaaatg tagcctttgt tatattggca
     1981 atcatactac tgtctaagat gttcaattct gaggaaatac tttttggtaa tagtaagagt
     2041 ttctctttct tagagaaaag aagtaatata aaaaaagggt ctataccaac tgtaagtgat
     2101 ggtgttattt tatatgcagt aggattaatt ctattgattt atgttggttc tttagttcaa
     2161 attaaatttg gtatggcagg tattataatg acacaattca tgataatttc tttaccatta
     2221 ttatttgcat actatattaa agctgatttt aaagaggtat ttagcttgac agttcctaaa
     2281 ttaaagcatg tgtttgggag cattgttctt tggattggtg gatacatact tattatgatt
     2341 ataacacagc taattttatt cttatttcct caaaatatgg aagttgctga aagtttaaat
     2401 aaggctcttt ttatcaaaga tagtgtttta ctgaatttac tagcaatagc tttattacca
     2461 gctatatgtg aagaaatgtt ttttagggga tttatatttt cttcatttag taaatcaaaa
     2521 gataaaaata agtctatcaa gctagctata atatgcagtg gggtactatt tggtattatg
     2581 catatggatt ttattagaat aattccaaca tcaatattag gtattatatt tgcatatagt
     2641 gtgtataaat ctggttcaat atttgtatca atattattgc attttttaaa taatagtgta
     2701 gcagttgtgt taaatcatta tacaactgga aatataacca acttatataa atttgttgaa
     2761 gttgattttt cgaacttaaa tgtacttaaa tttgccttaa ttcttctaat aagtcttata
     2821 ttaattatgt taggattaag agttttagat agaaagtctt taagaaatta agtataaagt
     2881 tattatatag attaaaatat taataagcac taaagtatat ggatatatct tgtatataaa
     2941 aatactttag tgctatttta tatattttaa ttatatgaat tatttatcgt ttcatttatg
     3001 taatgaaaat gttatataaa tgaaacaata aataattttt attttagaaa tttatattta
     3061 aataatcaag ttgaaagtga gatactaaaa aaattaattt aggaattatt tgatttattc
     3121 atttataaaa attaattact gatttgtcga aaccattgta ataagaaaaa aatgaaaaa
//
LOCUS       DAKBNN010000296         3167 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000296 DAKBNN010000000
VERSION     DAKBNN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.302"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     misc_binding    <1..252
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            328..2994
                     /locus_tag="OUB02_003691"
     CDS             328..2994
                     /locus_tag="OUB02_003691"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCT8887408.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1256 a          352 c          616 g          943 t
ORIGIN      
        1 gaagaagagg agtaagtaaa tactactaaa agagaggaaa attcactggc tgtaagattt
       61 tctaagtata agatttactg aatgtagctt tggagtttct aaactgaact ataagtaagt
      121 ttagacggtt agaaccgtta aattctttga gagtcaaagt tgataaaata acattaacca
      181 ataatttaat tatatgttaa tgtattttaa gctttggaat taaggtggta acgcgagctt
      241 ttcgtccttt ttaaagagga tgaagagctc ttttttattt ctaaagatat ttaaaaaatt
      301 tatttataaa atacaggagg caggacaatg gaaaatacga atttatcaaa aacttataac
      361 ccaaaagatt ttgaggctag gctatataaa aaatggatgg atgaagggta ttttaaatct
      421 aaaccaaatc cagataaaaa accatttaca ataatgatgc caccaccaaa tataacaggg
      481 caattgcata tgggacacgc tcttgaccat acacttcaag atatattaat cagatggaaa
      541 cgtatggatg gatatgaagc tttttggctt ccaggaactg accatgcttc tatagcaaca
      601 gaagttaaag ttgttgaaag aataaaaaaa caagaaggca agacaaagta tgaaataggt
      661 agagaagaat ttttaaagag agcctgggaa tggaaagatg aatttggagg aaaaatatct
      721 aatcagctta aacaactagg tgattcttgt gattgggata aagaaagatt tactatggat
      781 gaagggtgta atgaagcagt tatagaattt tttgtaagcc tatatgaaaa aggtcacata
      841 tacagaggaa atagaataat aaactggtgc ccagattgca aaactacttt atcagatgca
      901 gaagttgaac atgaagaaca cgacggaaac ttttatcata taaagtaccc actaaaagat
      961 agtgaagact ttttagaaat agctacaact agacctgaga caatgatagg agatactggt
     1021 atagcagtaa atccagaaga tgatagatat aaacatttaa taggaaaaac agcaatactt
     1081 ccattagtag gaagagaact tccaatagtt gcagatagtt atgttgattt agagtttgga
     1141 actggtgcag ttaagatgac tccagcacat gaccctaatg attttgaagt aggattaaga
     1201 cataatcttg agcagttaaa tactatgaat gaagatggaa ctatgaatga agtttgtgga
     1261 aaatatgaag gtatggatag atttgagtgt agaaaagcta tcgtagcaga cttaaaagaa
     1321 caaggttacc taattaagat aaaagagcat aatcataatg taggaacttg ttatagatgc
     1381 catacagtag ttgagcctag attatcagag caatggtttg ttaagatgga agaacttgca
     1441 aaaccggcta tagatattct taaaaaagga gaattagaat ttgttccaga taaatttgat
     1501 aaaacttatt tacaatggct agaaaatata agagattggt gtatatcaag acaattatgg
     1561 tggggtcatc aaataccagc ttattattgt caagagtgtg gagaaatagt agttgctaga
     1621 aaaatgccag ataagtgtcc taaatgtgga agcactcatt ttaaacaaga tgaagatgct
     1681 ttagatactt ggttcagttc tgcattatgg cctttctcta ctctaggatg gccaaataag
     1741 actgaagctt tagattatta ttatccaaca agtgtacttg ttacaggata cgatataata
     1801 ttcttctggg tagttagaat ggcatttgca ggtatgttct gtatgaatga aaagccattt
     1861 gaccatgttt tggttcatgg attagtaaga gattctcaag gtagaaaaat gagtaaatct
     1921 ttaggaaatg gtatcgaccc attggagata atcgagcaat atggagctga tgccctaaga
     1981 tttacattaa caactggaaa ttccccagga aatgatatga gattctatat ggaaagagta
     2041 gaatttgcta gaaactttgc aaataagttg tggaatgcat caagatttgt atttatgaat
     2101 attgatgaag atataattaa aaacatgact agagaaagtg ttaaagagga cttaactcta
     2161 gctgataaat ggataatatc aagagcaaat aatattgtaa aagaagcaac taataacatg
     2221 gataagtttg atttaggaat agcacttcaa aagatatacg attttacttg gtcagaatac
     2281 tgtgactggt atattgaaat ggttaaacct agattatatg gagaagatgc aaatgctaaa
     2341 tctgcagcat tatatacatt aacttatgtt cttgaaaaaa tattaaaatt attacatcca
     2401 tacatgccat ttataacaga ggaaatatat actcatcttc ctactgttga aggatgtata
     2461 atagtatctg aatggccaaa atataatgaa gaagataaca tggctgaaga agaagatatg
     2521 atgaacttat tgatggaagg tataagaagt ataagaaatg tgagagcaga aatgaatgta
     2581 ccaccttcta agaaagctaa attgataata atcccaagtg aagaaaaaat agaagctatt
     2641 gagcttggta aagactattt tataacttta gcatctgctt caaatgtaga gatagcaaaa
     2701 gataaatcta atgttccaga agatgcagtt ggagtagtta ttgatggtgt tgaaatattt
     2761 atacctctta atgaattagt tgattttgaa aaagaaattg agagattatc taaagaaaag
     2821 aaaaagttag aaggcgaaat aaagagagtt aatggaaagc ttgcaaatca aggtttctta
     2881 gcaaaagctc ctgaaagttt aattgaagaa gaaaaagcaa aaaaagaaaa atttgaagag
     2941 atgattaaat ctgttgagga aagattaaca aatttagaat ctaaaataaa ataaatataa
     3001 gaattagtaa aaacatacaa aaagtatata ttctatactt tttgtatgtt tttctttata
     3061 tatatacaaa tatgatgtta taattcttta cagatgtgtt atatttttat tttatgttgt
     3121 gaatatatga attttatgtc ttaaaattta taagcaataa aaatttg
//
LOCUS       DAKBNN010000297         3165 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000297 DAKBNN010000000
VERSION     DAKBNN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.303"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            529..2340
                     /locus_tag="OUB02_003692"
     CDS             529..2340
                     /locus_tag="OUB02_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HCT8887409.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAVLSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQIENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2428..3009)
                     /locus_tag="OUB02_003693"
     CDS             complement(2428..3009)
                     /locus_tag="OUB02_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887410.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     YSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1210 a          309 c          429 g         1217 t
ORIGIN      
        1 taatttatta tccatttata aattttattt ttaaatataa ttaataagaa ataagtaaat
       61 tattggtctt attacacaaa aatagatata atttaagtaa tataccaaaa aaaaggaata
      121 tatttacata atatgataaa ataaattcaa agagttaaat tgtaggtaaa tagtatttta
      181 attatttatt aaaatataga ttatgtatat gccaaattta aaaagagaca taaagacatt
      241 atacaaatat attattgggg tgatatttta taagaaaaga agtgatgcag taaattaatt
      301 gagcaattta aaaatgattt aatgatagtg ttgatgaatg tttaaaaaga aattttagaa
      361 aaacgtatat tgctgtttgt gttggaggtg atgaatttgt ataatttata ggatataaat
      421 gattaaacta tttaagataa atacatatca ctgtaaaata ttgataatca ttttttataa
      481 gagattgatt atttgcaaat tatatatatt acaaatgagg taacaataat gaagaaaaaa
      541 tatgattttt taatttattc tgctatttgg ctagttatta ttattctttt tatattttct
      601 ctatatcata gtatagagca tattaaaatt ataagtcaca ctggtactat tcgcagtgaa
      661 actcaaaagg ttgttaagca agaattgaat aatgaaagaa atgatgactt gataaaacga
      721 ctggataata ttctgataaa gctaagaaca ggtaatggag aaaatggatt tcaaagatgt
      781 gataataaag aatttcaaca aaaactaaat caaatggatt ctatgtggga atctatgaag
      841 aaagaaatta taaaagttag aaatggtgct tcaggtgata aattatataa attgagtgaa
      901 gagtattctg tgttgtctaa tcaaattgtt tttatatctg aaaaacactc taatgcaaaa
      961 ttgtattctt ttgctactgc actatttatt tatcttatat tctcaacaat aagtctttta
     1021 atttgggaat attataataa aaaaaggttt aaaagaatat tttatactga taatttaaca
     1081 aaaataaaaa atcaggttgc atttgaaaac agagcgattg aaattttgca caatgcatct
     1141 aacaaagagt atgtactgct aaatattgat attgacaatt ttaaatatat taatgataca
     1201 catggatatg aatatggaga taaagttcta ataatagttg cagctgtact ttctaaaact
     1261 tttaatatca aagaaacttg tgcaagaatt ggttcagata attttgtaat acttgcaaaa
     1321 tatagagatt ctcttttaga ggatattcgg gaaatgctaa ctaatgctat aatctctgaa
     1381 ttagatatga atgtaactca aactatatct tattgtatag gagcgtactt agttgaaatt
     1441 gataatttag gttataaatc catcaattct ataatggata aggcaaatat tgcacacaag
     1501 gtaagtaaaa caagaggaat ttcctccaca gtatggtata atgaaaatct gttaaaacag
     1561 ttacaaatag aaaatagtat ttataattat atgtataaag cgatagaaaa tgaagaattt
     1621 catatgtatt tgcaacctaa atttcaaata tcttctttaa atgttgtgag cgcagaagct
     1681 cttgtaagat ggttttctcc agaacttggt tttttatcac cagatgaatt tattccattg
     1741 tttgaaaaaa gtggatttat tattgaattg gacttttata tgttaaaaaa agcgtgttca
     1801 tttgtaagaa aaacgtttat gaaaaaaaat caatatacat atccgattgc agttaatttt
     1861 tcaagggtta ctatttacca aaatagtttt tatcaaaggt ttttagatac tgttaaagaa
     1921 tatgaaattc cttttaagta tatagagata gaggtaactg aaagtgcttt taatgaaata
     1981 tctcaaccag ttatttcgat tttagaggag cttaaaaagt taggattttt aatttcaatg
     2041 gatgattttg gatcaggata ctcttcatta agtcttttat gttctctatc tattaatgga
     2101 ttgaagttag ataaaagctt acttaaagaa acatttaacc gggagaaagt atatagtatt
     2161 attcagtgta ttattgaaat gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt
     2221 gaaactaaaa aagacttgga atttttaaat acagttaaat gtgatgtagg acaagggttt
     2281 tatttttcta aaccgataga agaaaaagaa ttttttaaca aatatgtaac taaaaaataa
     2341 tataattatt tttatcagtc tatacattga gtcattttat atctcgttaa atgactcaat
     2401 gtattgttat gtattttaaa atataaatta tttaaaacgt tttttgtact tatatagaaa
     2461 ttcttctata ttttttgatt gttctcttat ttcaactgct tctatatcta gtaaataaag
     2521 tatagaataa tttgtaagtt caacatattc accatcttct gaaccgagtg catgtctctc
     2581 aatttttaat cctttttctt cataatattt ttttgtttct atatatagtg attctcgctt
     2641 ttgtatgtag tctttcactt cattatttgg aaagttatat gaatccacta atgtttcgcc
     2701 atttataaaa gttttctcgg tagcaacttt ttcatcccaa caatggattt cttcccaagt
     2761 aaaaaatatc tcagttccat ctggaatgaa tgaaaacctg gtaataatat ataaataatt
     2821 attgaaattt acttcataat attcaggaat atcttcaaat tccatatagt aatttgtata
     2881 attaaaatta ctattatctt tttttacaaa gggtattttt tcaataaatt tatatattga
     2941 ttgttcattt ttaaaaattc ctatatcata acttttttca aattgattaa tttttaataa
     3001 atacaacatt ctttctcctt ttattcaaaa tataattttt ataaatttac taatctttag
     3061 cttttttaat tatatattta catatgttta tatgatacta taataatgtg ttttaaatag
     3121 atatataata gtaaatattt tttatatagt tatgttttag taaaa
//
LOCUS       DAKBNN010000298         3160 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000298 DAKBNN010000000
VERSION     DAKBNN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.304"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            454..1377
                     /locus_tag="OUB02_003694"
     CDS             454..1377
                     /locus_tag="OUB02_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HCT8887411.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIESDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SATYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNILKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1352..2179
                     /gene="panB"
                     /locus_tag="OUB02_003695"
     CDS             1352..2179
                     /gene="panB"
                     /locus_tag="OUB02_003695"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCT8887412.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            2198..3046
                     /locus_tag="OUB02_003696"
     CDS             2198..3046
                     /locus_tag="OUB02_003696"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCT8887413.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAI
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1280 a          366 c          476 g         1038 t
ORIGIN      
        1 acataataca tctctctctt atattaaatt ttaacttggt actaatcttg acaaattgtc
       61 cacaaaaata aattatatat tttattattg ttttaaattt ttataaaaaa tcagtattat
      121 tgatactatt gaagttaaaa agcattaatt tctaaaaaca tcataatttt tctaatagac
      181 ttaatcactt ttaatatata tagtaaaaca aaaaacttat attttataaa tatcataatt
      241 tttttactga tattttatta tataaatttt atagaattaa ttgtcaattt tttatgaaaa
      301 tggtaagata tttatggatg gtatcttgtt ataagaacta tactttaaat gtttttaatt
      361 attttgtctt atgtaactca tatcctcagg aatgagatta gaaattaaga tgttgtatca
      421 caaagtggag gtgatactat taaaataggc tttattggtt ctggaaaagt tggaacatct
      481 ttggcaaatt attttttatc aaaaggttat tgtgtttcag gcttttatgg aaggaatcaa
      541 ttatctttat tagaatcaac aaatttaaca aaaacaaaga tttacagcaa tttgaaagat
      601 ataatttatg aaaacgatat attatttatt acaacatcag atgattctat tgaaattata
      661 gacaaaaaac tttccaaatt taatttaaaa cataaataca tttgccacac aagtggctct
      721 ttaaaatcta gtatattgtt ctgttcaaaa aaagcaggtg cattaattta ttctatccat
      781 cctatatttg cattttccaa taaaaatacc aacataaaaa aaatgaaaga catttgtttt
      841 tccatagaaa gtgataatga acaagatgat ttagttattc aaaatttcat agatgatttg
      901 ggaaaccaat tttttataag agataaggat acttcagcca cttatcattt agctaatgtt
      961 ttagtttcaa atttagtatt atctttacta gatataggag taagttactt tattaaatta
     1021 ggtttatctg aagaggaatc tttaaaagct atcagtcctt tagtaaaaaa aaatatagaa
     1081 aatatacttg ataatggctt tactaaagct cttactggtc ctgtttctcg tggagatata
     1141 acaccaataa gaaaacatct atctgtattg gataaaaaag atgaggatat ttacaaaata
     1201 ctatccttaa acatactaaa aataattgcc ttaggaaatg acaattcatt aaaaggtata
     1261 aaaaatatta ctatagaaaa tgcaatcgaa aatttaataa gtaaatctga aaaatacaaa
     1321 gaaatttaca aacttttagg aggaaaaaac gatgaaaaat accatacaga cttttaagaa
     1381 tgctaaatat gaaggaaaaa aactatctat gcttactgct tatgactact caattgcaaa
     1441 aataatggat gagtgtgata taaatggtat tttaatagga gattcattag gaatggttat
     1501 aaaaggtgaa gaaaacactc tatctgttac catagacgaa attatatatc atacaaaagc
     1561 tgtaaaaaat ggtgttaaaa atgccttaat cgtaagtgat atgccttttc tatcttacca
     1621 tgtttctata gaagatgcag ttaaaaacgc cggtagactt ataaaagaag gaggagctca
     1681 tgctgtaaag ttagaaggtg gctcaaacgt tataaagcaa atagaaagca ttgtaaatgc
     1741 tcaaatacct gtaatggggc atttaggatt aactcctcaa tcagtaaatt catttggtgg
     1801 gtttaaggtt caaggaaata ccagtgaaac agcaagacaa cttattgaag atgctaaatt
     1861 aatagaaaaa gcaggtgcat tctctattgt ccttgaagga gtgcctacga aaatagctga
     1921 gatggtaact aattctattt caattcctac aataggtata ggggcaggaa ttaactgtga
     1981 tggacaaatt ttagtttatc aagatatgtt gggaatgttt ggagattttg ttcctaagtt
     2041 tgtaaagcag tatgcaaata taggagatat aatgaaagat tcaattaaaa attacatcct
     2101 agaagttaat acaggtgcat ttccacaaga aaaacacagc ttttcaataa atgaatctga
     2161 gttagaaaaa ttatactaat attaaggatg gtacaaaatg cttgtaaaag aaattaaatt
     2221 actaagaaat ataatcaaag attggagaaa acatggctat tctatagggc ttgttacaac
     2281 aatgggattt ttacatgaag gacaccaaag tcttataaaa aaagctataa aagaaaatga
     2341 taaagtagta gtgagtgtat ttgtaaatcc aactcaattt ggaccaaatg aagattttaa
     2401 tagctatcca agagatatag ataaagattt taaatattgt atggatagtg gagcaacagt
     2461 agtctttaac ccatcaccag aagaaatgta tttaaaaggt aattgtacta ccattaatgt
     2521 ttctggactt acagattttc tttgtggagc taaaagacct gttcattttg gaggtgtctg
     2581 cctagttgtt tcaaagtttc taaatatagt aacacctgat aaagcttatt ttggagagaa
     2641 ggatgcccaa caattagctg taattaaaag aatggtcaag gatttaaaca tagatacaga
     2701 aataataggc tgtcctataa ttcgtgaaaa tgatggatta gcaaaaagtt cgagaaatac
     2761 atatctctct gaggaagaac gaaaatctgc tctaatatta aataagagct taagtttagc
     2821 aaaagaagaa ttagtaaaag gcaatctaaa tccagagaat ataaaagaat taataacagc
     2881 taaaataaat tctgaacact tagcaaaaat agactatgtt gaaattgtag atagtgagac
     2941 attacaacct gttaagcaaa tagaacattc tatacttgtg gcaattgctg tgtttatagg
     3001 aaaaaccaga ctaattgata attttacatt tgagttaaat atataatcta taattttata
     3061 taatatatct aaaaaataat agaataccta aaaagtaata aattacctaa aaaataataa
     3121 attgcttaga aaataataga ttacaataac taaaatttta
//
LOCUS       DAKBNN010000299         3117 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000299 DAKBNN010000000
VERSION     DAKBNN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.305"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..572)
                     /locus_tag="OUB02_003697"
     CDS             complement(<1..572)
                     /locus_tag="OUB02_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCT8887414.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTD"
     gene            complement(572..1432)
                     /locus_tag="OUB02_003698"
     CDS             complement(572..1432)
                     /locus_tag="OUB02_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887415.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1567..2367
                     /locus_tag="OUB02_003699"
     CDS             1567..2367
                     /locus_tag="OUB02_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HCT8887416.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2392..2961
                     /gene="yfcE"
                     /locus_tag="OUB02_003700"
     CDS             2392..2961
                     /gene="yfcE"
                     /locus_tag="OUB02_003700"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCT8887417.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1164 a          462 c          380 g         1111 t
ORIGIN      
        1 atatcagtta cataatttga aagatatact cctccaactt tactataatt aaaatgcttt
       61 acttgatata caatcatact aatcatcttc aaaagacttc catatacaaa gtaaattaaa
      121 atacttacta taaaaccaca aactttcatt tttccatatc caaacttaaa aattaaaggc
      181 agtaaaatcg tcaaaattgc taaaaatata tttacagaaa aaaatacatc atttaaaacc
      241 atatttctaa agaacactag tgatgtcaat ttacttagta aggacacaat tattgttaca
      301 gaaattatta atcctacacc taataaatat tttgagtaaa ctatatcttc tatactaact
      361 ggcatactcc ttataaaaat ccttgaatca ttttcataat cacactcaaa cgtatttata
      421 aatatcaaat aactaacaac aatacttact gtataatatg aaattgaatt aaggaaaaaa
      481 taaaacacaa aaaatagcaa tatgtacttt aaattacgga taccatctga actaatccct
      541 attattaaat cttttcttat taaattgaac atcatctcac ctcatttaca taataataca
      601 ttagttcttc tatagtagga tttttgacct tatgcttatc aatttctatc aataggcttt
      661 ttactttcaa gctatctcca actttaacaa gtgcctcatt ataatattta tactctttca
      721 caccaactat gctttcttta attgcttcta actctgcttt atttgcccta attatcttat
      781 attcttgttc tattaattct ttcttaacag taaaaatctg atttcctcta tttataaaaa
      841 caagataatc ggcaatttta attaaattat cagtattatg agtagaaatt attacactac
      901 tacttccatt tttcacaaaa tcttgtaagt actgcattat ttcagttctt acaattgggt
      961 ctatcccagc agttggttca tccaaaatca ataacttagc attgtgacta agtgcacttg
     1021 ataacataag cttttcagat tgacctttag ataaattttc aatatgcata ttttcttcta
     1081 tatcaaactt attaaggtat tcatcaaacc tttctctatc aaaattctta taaaataaag
     1141 aaactatttt tctaaattcc ttaaccttta aatgcggata aaattgtaaa gaatcatata
     1201 caaaaccaat attgttttta aaacttattg gagaattttc aatagtttca ttacaaagtt
     1261 caatttcacc ttcatctgca tcaataatcc ccattataat ttttattaaa gtcgtttttc
     1321 ccgaaccatt tggtcctata atcccaacaa tatccccttc ctttaactct aagtttatat
     1381 tatttaactt gaattttcct aatttttttg tgatattcct tatgctaaac acatcttcac
     1441 ctcttatgca tttttttata cccacctata catattttaa cacattcatt acaaaggtca
     1501 tttataaatt tataaattaa gaattataaa actatgttat aatacaaagt atgaagaggt
     1561 gaatatatga aaagaattat aactataaat acaaaccaat taaataagtc ccttgagact
     1621 aaagaactct taactagaaa acttatcaat gctggctttg aagtgtattc agatatttat
     1681 ccagacacag agcttattat atcaattggt ggtgatggct cttttttaag aacagttaga
     1741 gatttcgatt ttcctgagat tcccataatg ggaataaata caggtcatct ggggtttttt
     1801 cctgatattt taccagataa gattgatagc tttattgaag cttatacaaa aaaagattat
     1861 attattcaag aaatgtcact tcttaatgcg gaagtatata caactactag tggttctaat
     1921 atgcttgctg taaatgaggt tgttataaga ggagacaagt ctaggactat acacctaaat
     1981 ttaagccttg ataataaaca tatacaaaat tttagtggag atggtatgat tatttctact
     2041 tccacaggtt caacagccta taactattct gctggaggta gtattgttga tataaatctt
     2101 gaacttatgc agattacacc tttacatccg ataaatacca atgcatatag gtgctttaca
     2161 tcaagtataa tatgttcaaa tgaatctgtt ataaagattg caccagaata tagatttgaa
     2221 gattctgtat tgattgtcgt tgatggagtt gagcacagat ttagacaaat cgaaaatata
     2281 aaagttagta tatctgatgc aaaaataaaa ttacttagaa tgtcaaacta tgagttttgg
     2341 catagagtat ctgaaaaatt tttatagact gtacttagga ggatttttaa tatgaaaata
     2401 ggaataatga gtgatactca tggtagctta ctttactttg aaaaagcgtt aaatgtatta
     2461 tctgattgtg atgttttatt acatggtgga gatgtcttat atcatggacc tagaaatgat
     2521 attccagaag gatataatcc taaaaaattt attgagacat taaataaatt agaaaacatt
     2581 gtgattgtaa aagggaactg tgatgcagat gttgaccaga tggttataga acatcctatt
     2641 cagagtcctt atgtcatgag ccaatttggt gaaattagaa ttatattgaa tcatggttat
     2701 atagaatcag aagaagaaat tattgacaaa gcaaagaaaa tgggtggtga catattagtt
     2761 ttaggacata ctcatgtaaa aaagctatac atggatgata atttaatagt aataaatcca
     2821 ggaagtactt ctatcccaaa agatggaagc cattctgtcg caattataga tatcataaaa
     2881 actgatgatg aggatgagct tgaacttgat ataaacttaa ttgacatcaa tactggaaat
     2941 ataataaata tcaatgatta gtaaattttt aataaaaata aaataagctt taatttatta
     3001 aactcataat aaaaacaagt aaaccatttt atttctatct caattagaag tttaagtaca
     3061 ataccttaaa cttctaatta aatagaatct ggtttacttg ttttattata tgctata
//
LOCUS       DAKBNN010000300         3085 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000300 DAKBNN010000000
VERSION     DAKBNN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.306"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            125..703
                     /locus_tag="OUB02_003701"
     CDS             125..703
                     /locus_tag="OUB02_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8887418.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNKLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            994..2244
                     /locus_tag="OUB02_003702"
     CDS             994..2244
                     /locus_tag="OUB02_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8887419.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            2405..2974
                     /locus_tag="OUB02_003703"
     CDS             2405..2974
                     /locus_tag="OUB02_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCT8887420.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         1155 a          404 c          455 g         1071 t
ORIGIN      
        1 aatataccaa tattataaac ataaatcact tgacaaattt ataagtagac aatattatat
       61 atctatgact caaaatattt tcaaagtcat atatatcata tatagacaga aaggattgat
      121 aaaaatggct aaaagtttta cagaaataga aaaagacaat ataagaaaaa gactaatttc
      181 agaatgtgaa atcaactggt ctaaatatgg atacaaaaaa actaatattg atgaactttg
      241 ttcaaaatca ggaattgcaa agggttcttt ctatacattt tttgattcaa aagaaaagtt
      301 attttttgaa gtattaaatg gaatccaaaa caaacttata tcatcagtag atgaaatttt
      361 aagtaacatg cctccaaaag aaggctttgc aaaatcctta aaaatgttat atcgattata
      421 tgataaaaat ccatttttat attcaccaaa taatcctgat ttcatcagcc taataaataa
      481 actacctcaa gatatgtttt tacaacttga gtccaataac atacaaagtt tttacgactt
      541 aattgagaga cacaatctca agttgaagat agaaaaagaa aaagtttttg gtgtttgtaa
      601 tgctctacta tcaagtctat tggtaaaaga aaatattgaa tataactata ttgaagtgtt
      661 tgactttatg gtggataatc ttgtagaaca catacttgaa taacaattta aaataatttt
      721 tctaagtatt tttttatact aaataggatt aatatgattg aattaatgaa aaaaataaac
      781 aatataattt ataaaataaa tgataaacta aatatcacac aaaacttata gaccctagag
      841 caaacccatt ccactctact ctaagccatc tataatagtt tgtatatttc taataatatt
      901 agatattaaa gtaagaaaaa aattccttat aataattgct attatatcaa ttattataaa
      961 tattttaata atcttagata ggtaggtaca tatatggtaa aatcctcgaa cacttttaat
     1021 aaaaacttca acttgatgat attaggtcaa ataatctcat tgtttggtgc atcaatactc
     1081 aagtttgctt tgtctttata catacttgat ataactggta aagcagaaat atttgctact
     1141 atactagcag tatcttcaat cccaattata gtattctccc ctattggtgg agctattgcg
     1201 gatatattca acagaagaaa cttaatggtt atttttgatt tttcaagtag tcttacagta
     1261 ctaatcttag cagtattctt attcaacaat aatggttcaa tattacttgt aggaattatt
     1321 atgaccatat tatcagtcat aagcacaatg tatcaaccaa ctgttcagtc aagtacaccc
     1381 cttttagttg ataatgagca tttaatgaat gcaaatggta ttgtagctgg agttgcatct
     1441 cttacaaata ttgcaggacc tgtattagga ggtgtcttgt atggtgtgat tggaattaat
     1501 gcaattatag ttataagttg tgtaagtttc tttctatctg caattatgga gatatttata
     1561 cagattcctt ttatcaaaca agagcaaaac agcaacatta taaaaactat atttactgat
     1621 ataaaggatg gtattgttca tatatgtaaa gaaaatagat ttatactaaa aatacttttt
     1681 ttagctatag caataaactt atttttaagc tctatgttaa tagttggtat cccctatata
     1741 gtaaaaatca ctatgggagc tagtaacttc ttatatggtc tagcagaagg agcaatttct
     1801 tttagttcac tggtagctgc tgttagtatt ggtattttct caaaacatct aaaaatcact
     1861 aatttgcaca tttcctttat tatatgtgca gttatgttca taccaatggc ggtttcagta
     1921 tatccattta tattaaacac aggatttcca gttccatttt taatctttat agtatcatct
     1981 atgctaatat tttttacaac gactcttatc tcaatatatg ttataacagt cacacaatta
     2041 aaaactccaa atgaactctt aggaaaagta atgtcaattc tttttgcagg aacagcaatt
     2101 gcaatgcccc ttggtcaggt tatgtatggt aaactttttg atttattatc agatagagtg
     2161 tacatcatta tactaggagt tggatttatt acattactta tagcatttgt atcaaagata
     2221 acattgagga acgaaaatct ttagatacaa agaatttaga tataaagatt taattttcac
     2281 taatttctga atttaaatgc aaaattgtgt atcagtatgt tgacgtttta gaaattgatt
     2341 gatataatca atggaaaata aactttataa aaattcaaaa tattcagcta gaggagtgat
     2401 aaaaatgagt tacatgccaa ctttagaaca agcatgggaa atattaagaa aatacaataa
     2461 ggaagaattt catataagac atgctcaaat tgtatctgga gttatgagat actatgcaaa
     2521 ggagtacgac cctgaaagag tagaattttg ggaaatagtt ggacttttac atgatttaga
     2581 ttttgaaatg tatcctgatg aacattgtgt taaacaaaga gaattaatga cagagcttga
     2641 tttagataaa tcaataatcg attctacaat aagtcatgga tatggattaa ctggttcaga
     2701 tgttaaacca gagcaattta tggagaaagt attatttgct gtggatgaac ttactggttt
     2761 aattggtgct gctgcaataa tgcgtccttc aaagagcgtt tctgatttag aattaaaatc
     2821 tgttaagaag aaatttaaag ataaatcatt tgctgctggc tgttctcgag acgttataag
     2881 aagtggtgca gaaatgcttg gatgggaact tgataagctt atccaaacaa ctatagaagc
     2941 tatgagaagc ttgattccag agatggcaat atagctcctc catacatata ccatagaagt
     3001 tacatcttta aagagcattt ctctttttga tgcaacttct ttttatattt tgtagaataa
     3061 aaagtttaat attttaaact cacta
//
LOCUS       DAKBNN010000301         3050 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000301 DAKBNN010000000
VERSION     DAKBNN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.307"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            123..1409
                     /locus_tag="OUB02_003704"
     CDS             123..1409
                     /locus_tag="OUB02_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HCT8887421.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1495..2802
                     /gene="grdB"
                     /locus_tag="OUB02_003705"
     CDS             1495..2802
                     /gene="grdB"
                     /locus_tag="OUB02_003705"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2542..2544,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HCT8887422.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDGAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     DEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
BASE COUNT         1178 a          333 c          631 g          908 t
ORIGIN      
        1 tttctatgac attaatacat agaatatata ttttaaataa ttggcataga aaatgcttta
       61 tatattaata aacgtaataa atacttttaa ttgcgattaa atatattaag ggggattttc
      121 atatgaagct agagctaggg aatatattta taaaaaatgt tgaatttgga gaaaagacag
      181 aagtaaaaga tggagtacta tatattagta gcgaagaaat agaaaagata gtattagaag
      241 atgaaaggat tgtaagtgta aatgttgaac ttgcaagacc tggcgaatca ataagaatag
      301 ctccagttaa agatgtaata gagcctagag ttaaaattgg agatgaaagc aaaatatttc
      361 ctggaattat aaataaagtg aagactgtag gaagtggaag aacccatgta cttttaggag
      421 cttgtgttgt aacttgtgga aatatagttg gatttcaaga aggcgtaatt gatatgagtg
      481 gtcctactgc taaatataca cctttttcaa aaacaaataa tatatgtatt gttataaaag
      541 caaaggatgg tatagatact catgattatg aagaagcagc tagaattgca ggattaaaaa
      601 taggtgcata tgtaggtgag gcaggaagag aagttgagcc agatgaagtc attgtatatg
      661 agacaaagcc attattaaaa caagtaaaag aatatccaga cctgccaaca gtagcatatg
      721 ttcacatgtt acaatcacaa ggacttttac atgacactta ttactatggg gtggatgcta
      781 aacagatagt gcctacattt atgtatccaa ctgaaataat ggacggagct ataatatctg
      841 gaaactgtgt agcaccatgt gataaggtta caacattcca tcatttaaat aatccagtta
      901 tacatgactt atataaaaga catggaaaag atttaaactt tataggagtt attttgacta
      961 atgagaacgt attcttagtc gataaagaga gaagctctga tatggtagct aaattaatag
     1021 aatttttagg agttgatgga gttctagtga cagaagaagg ttatgggaat ccagatactg
     1081 acctaatgat gaattgtaga aaatgtagtg aagttggagc aaatgtagtt ttgataacgg
     1141 atgaatttcc tggaaaagat ggcaagagtc aatctatagc agacgctact aaggaagcag
     1201 atgcagttgt ttcttgtggt caaggaaatc tagtggttca tttcccagca atggaaaaaa
     1261 ttataggaac tttagattat gttgaaatga tgataggtgg gtataaagga tgcttaaatg
     1321 aagacggaag catggatgca gagctacaaa ttataatagc atcaactata gcaaatggct
     1381 ataatcattt gacagcaaga tattattaaa taagtatttg aatataaagt tctgttgatg
     1441 taatcattgg tctaactgat gtaataacta taaagtgatt tggaggatta agagatgagt
     1501 aaaatcagag tggttcatta tataaacaat ttctttgctg gtataggtgg agaggaaaaa
     1561 gcagatattc cgccagaaaa aagggctggt gtagtaggac ctggaatagc atttcagagt
     1621 caatttaatg atggtgcaga gataatctca actgtaattt gcggagatac gtattttggt
     1681 gaaaatattg agagtgcaac tcaagaactt ttaaaaatga taaaagaaga aagccctgat
     1741 ttatttattg caggcccagc atttaatgca ggtagatatg gagtagcttg tgggactatt
     1801 tgtaaggcag ttgaagaaga gttaaatatt cctgtaatta caggcatgta taaagaaaat
     1861 ccaggtgtag atatgtttaa attagatttg catatagtgt ctacaggaaa ttcagcagca
     1921 agtcttagaa aggtagttcc aataatgacg aaactaggtc ttaaattggt tagagatgag
     1981 gaaataggac ctcctgaaga ggaaggatat ataatgagag gaataagaaa aaatttcttc
     2041 catgaactta gagggtctga aagagcaatt gatatgttgg ttaaaaagat gaaaggggaa
     2101 agctttgaaa ctgagtatcc aatgcctgaa tttgatagag ttacacctat gaaacctgtt
     2161 aaagatttat ctaagataaa acttgcccta gtaacttctg gagggatagt tccaattgat
     2221 aatccagaca aaattgaatc ttcaagtgct actaaatatg gaatttacga tttaacagat
     2281 atggattcta tgtcaaataa agaatttaca actatacatg gggggtatga tagagcatat
     2341 gtccttgaaa atccaaattt agttgtacca ctagatgtag ttagagagct tgaaaaagaa
     2401 ggtgtaattg gagaattagt agattatttt ataactacta caggaacagg aacgagtgtt
     2461 ggcaattcta agagatttgg agaagagttt tctaaaaaat tgttagaaga tgatgtagat
     2521 gctgtaattt taacttctac atgaggtact tgtactcgtt gtggagcaac gatggtaaaa
     2581 gagattgaga gagctggtat agcagttgtt catatatgta cagtagtacc aatatcactt
     2641 acaataggag ctaatagaat tgttccagca gtagggatac catatccact tggaaatcct
     2701 atgttaggag aagaggaaag taaaaaaatt agaaagaata tagttttaaa agctttaaat
     2761 gcactaacag aagatataga agaacaaaca gtatttgaat aataaaaata caacacaaca
     2821 ctgaaataaa aaaagatatc aaaaaataat tttttaaaat tattttacga tatctttttt
     2881 tattaggtaa attcgaaagt tgcatattat ttgcatattt ttaaattaaa tttataaaag
     2941 tgataaatta gtttattata atattgatac acaaattcaa attagatata ctatgaaaag
     3001 tgagattatt acattttgga acaaatattc aacatattaa accagattaa
//
LOCUS       DAKBNN010000302         3008 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000302 DAKBNN010000000
VERSION     DAKBNN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.308"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            73..1182
                     /locus_tag="OUB02_003706"
     CDS             73..1182
                     /locus_tag="OUB02_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCT8887423.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1559..2743)
                     /locus_tag="OUB02_003707"
     CDS             complement(1559..2743)
                     /locus_tag="OUB02_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887424.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1138 a          448 c          408 g         1014 t
ORIGIN      
        1 aaaatactta taaatatgat attattaatt ggttaataaa aaagaaaatt tttcatgaaa
       61 ggtaagataa caatggattt caaaagctta gggattagtg aaaatacaat aaatattcta
      121 aaaaaatcag gaataacaac acctacacct atacaaaaag aaagtattaa actaataaaa
      181 gaaggaaaag atgtaatagc agaagctcaa actggaacag gaaaaacact agctttttta
      241 cttccgatat ttgaaaatat atcattagat ataaatgata tacaagtact tatactttct
      301 cctacaagag aactagctat tcaaataaca gaagaagcta tgaaacttaa agaatctaaa
      361 gatgtaagta ttcttgcagc atatggagga aaagatattg gttctcaaat aaagaaacta
      421 aaaggaaata ttcatatgat tatagctaca ccaggtaggc ttttagacca tctaaataga
      481 aaaacaatag accttagtaa gttgaagact tttgttttag atgaagcaga ccaaatgttg
      541 cttatgggat ttaaaaatga agtggaagct attttaaaag agacttcaaa caaaaagcaa
      601 acactatgct tttctgcaac tataaattct caagtaaaaa aattagctta tagatacaca
      661 aaaaatcctg ttgtagtttc tattcaaaaa gaagaaatca ctcttaacaa tataaaacaa
      721 gaagtagtag aaactacaga tagaaaaaaa ttagatgcct tatgtaaggt actagatgaa
      781 gataatcctt ttatggcaat aatattttgt agaactaaga gaagggttga caatcttgaa
      841 gaagctcttg ctataagagg atataattgt caaaaacttc atagtgatat tgctcaatca
      901 aaacgagaaa gaataatgaa atcatttaga aatttagata tacaatactt aatagctaca
      961 gatgtagctt ctcgtggtct tgatatatct ggagtaagtc atatatataa ttacgattta
     1021 ccagaaacac cagaagacta catccaccgt attggtagaa ctggtagagc tggtgaagaa
     1081 ggctatacat gtgcttttat tgaccctaaa aatgaacgaa tgttaagtga aatagagacg
     1141 gctatagaat ctaaaattag tagaagaatt atagaattat aatttataga ttgcttaaaa
     1201 taccaatgcc acacaaacac tgtaatatat agaaagctac agaaaacatg tatcaatcta
     1261 tactttgctg ttatactatt ttatattaaa gaaaaaagag gctatccaaa gtaggattag
     1321 tctcttttat tgtcaaaata atgaaaatat atttgacttc atataaaaaa gagtgcctta
     1381 taaactaaaa gtccattttg agacaccctc tttttatctt ttattcttag ttttatattg
     1441 cttgtttata gttgtttaat attagatttt gcaaataatc ttaataaaca tgcagatata
     1501 ttatctatag tattcttatc tatagtattc tctttagcaa tattttttgc tactacatct
     1561 aatttacatt ttcatttaat tccgctggcc atttagcttc tggattttcc tttgcaaact
     1621 tcttattctt ataatgtcct agtgtaagga atggaattac tatagtgaat agaccaagat
     1681 aaccacaata tccatatcca tatttaacaa tattacttaa tcctgtaagt gatataagca
     1741 ttgacactac cattattgca caagctgtaa atacccttct tttcattctt gaagaaagga
     1801 aagaaagtgc tttatggtct tcaaatctat ttacaagacc aaatatagta gttactcctg
     1861 tagacattaa acaaaggaat aatgcaacat tataaattat gtatatagta tgatttccag
     1921 attgacctgc aacataaaga gtagggagtg ttgtttgccc agcagcaata atttctctat
     1981 accatcccat tagcatagtt acagcaagtc ctaatgcaat tgcattcata acaaatgaaa
     2041 ttatcattgc cttagtagct tcttttgagt tcttaagaag tggtcttgaa catcctgcaa
     2101 gtgtaggtat aacaaccgat tgaaaacctg cataaataaa agtattaagt attggcttga
     2161 tatagccagg tgtgaaactc gttctatttg ataatatttc agttatttct ggtactttag
     2221 cttttatacc aacaataaaa atagtaagag tacatactag aatagctatt gtcattgctg
     2281 tagctgcttt agatacaagc atagcaccaa atatagtaaa gacaagaagt acaactccaa
     2341 taataaatac agctacaaaa taatttactc ctatactttg aaatactgct gctgcacctg
     2401 ctataacagc acttacagct attaaaacca ttagatagta atatatctcc catattattt
     2461 caagttttgg atatggtctc caaagctcac aaaataaatc cttataattc ctacaattgt
     2521 tattattata cattatgata acttctctca tggtagcagt aagtataatc attgctaaaa
     2581 ttgccataag tggtgctgtc catccatact gcacatagta ctgagtttct tgattccctg
     2641 tagcaaatcc tcctcctgca tgtgaactaa aaagaacgct tgcaacacca aaaatagcta
     2701 acatagagcc cgttactttt gctttcttta cacttttttg catgaaatca tcctccctaa
     2761 aatgatattg aaagttttta ttttaacact ttattgataa aacaataaca tttttgctca
     2821 ttaaattcaa ctatattttt ccaatgactt tacattttca acttatttcc acctttatat
     2881 taaatatttt ttatatattt attttatttt ttaaatagta ttcaaattca ctttgttttt
     2941 taattttatg aaatatcttt tttaatgtta aatattatac attttttctt tattattata
     3001 taattttt
//
LOCUS       DAKBNN010000303         2999 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000303 DAKBNN010000000
VERSION     DAKBNN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.309"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            309..575
                     /gene="rpsT"
                     /locus_tag="OUB02_003708"
     CDS             309..575
                     /gene="rpsT"
                     /locus_tag="OUB02_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCT8887425.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(762..1805)
                     /gene="holA"
                     /locus_tag="OUB02_003709"
     CDS             complement(762..1805)
                     /gene="holA"
                     /locus_tag="OUB02_003709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCT8887426.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1759..>2999)
                     /locus_tag="OUB02_003710"
     CDS             complement(1759..>2999)
                     /locus_tag="OUB02_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCT8887427.1"
                     /translation="ISFIITNNTKNDEELNDKIITIQGVVKGKIEKKRYNQYKVGVFL
                     INDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGRYIKSTGYKGIVYINNYKIIDK
                     NKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEVENLTEEQKEIFTRTGTSHVIS
                     ISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYCIMVGASPSIIRSIAFVMIFYL
                     SIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLSFLATLSILIFYNKINSIIKLS
                     MVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVGVIMYLSIASLIVFKVSVVI
                     AKIISFFNSTLIESIFFLLEKISNLSFAYINIENPKFYIVVIYYIGVFFYIFYIEGKE
                     IKEQENELQGYYKECKREKL"
BASE COUNT         1092 a          430 c          282 g         1195 t
ORIGIN      
        1 ttcttgtatt atcttataat tattggaaat actatcaaaa tgtatgttaa tagtttgtat
       61 tatctcaaaa tatttgtttc aaaaataaaa tattaattta tatatgtcaa aatatttaaa
      121 acacaaaata taccaattta aaaacaagat attgcaaatt atttatacac aatttacagt
      181 ctaaaaaatc aaaataaaaa acattattaa aatatataaa taataatcct tgatttttag
      241 atttatttct gttaaaatct aatatgttga cgatgaatat gataaaaaaa tacagggagg
      301 tgaacacggt ggcaaatata aaatcagcta agaaaagaat aagtgttatc gaaaagaaaa
      361 ctgctttaaa tagagttaga aaatctcaaa taaaaactgc aataagaaga ttcgaagatg
      421 ctgttgcagc tggaaacaga gaggatgctg ttgctaagtt ccaatacgct caaaaaagaa
      481 tataccaagt agcttctaaa ggaactatac ataagaatgc tgcagctaga aaagtagcta
      541 aattagctca aaaattaaac ggtatgaacg cttaattacc agtttatatt tcttatatat
      601 aataacccct atgtaggggt tttttatttt gtaaatttat acaataaaaa ataaatagtt
      661 agacatatta tagttgattg tatatcaaac ttaatttagc ttacatctac tctgaactat
      721 aacatatctt gctatttatt ttttattaat atatagattt tttaaattgc ttctctttta
      781 cagtatctac taaccaacat ttcaactgct aaagtatctc ttataagacc tgtctttatt
      841 ttgtaatcac tttctaatat atagtttaat atttccacaa ttatatcatc agaaaaattt
      901 ttagtttgct ttaatgcttt tcctacaaca aattgatgca tttttaattt atcagctata
      961 agttttgttg aatatccatc aagttgtaac tgtcttactt gcataattat tttaaattgt
     1021 cttgctatca tagaaaatat acctaataca gattcgcctt cttgtatcat atcatttaat
     1081 attttcattg cattagaagc attttgttct ccaatataat cgattaattt gaatatatca
     1141 ttctcaactt ttttttgaga taatttatca attacatcat tggttacatt attcccttta
     1201 ccaacaaatg aacttatttt atttatttca ttttctaaat cagataatgt tttttcacta
     1261 ctcttatctc tgtatccttc ttgttcaata aaatacatga tttgagaatt atctattata
     1321 acatcattta aagcaaaaga tttttttatc catttaaaca aatccatatc agaaagctta
     1381 tcacaatcaa aaacaattcc attgtttcct attttcttaa ctaatgattt tcttttgtct
     1441 acatctccat atacaacaaa aactatagtt gtggtgtctg gaatattgtc taaatgctct
     1501 attaagtatt tctcatcact atctgtaaaa ttctttttct ttccttttaa tagttcaaag
     1561 tcttttatta ttactatctt cctatcatcc ataaatggaa gtgtttctat agagcttata
     1621 agctgattta agactatttc cttcccatct attatatcta aattaaaatc aagcattccc
     1681 tcgtttagac tatctttaaa tgcttttatt gcattctcaa ttaaataata ctctcttcca
     1741 taaaaaagat acattttttc aaagtttttc tcttttacat tccttataat atccttgtaa
     1801 ttcattctct tgctccttta tctcttttcc ttctatgtaa aatatgtaaa aaaacactcc
     1861 tatgtaataa ataacaacaa tataaaattt aggattttct atattaatgt atgcaaaact
     1921 taaattgctg attttttcta gtaaaaaaaa tatgctttct atcaatgtac tattaaaaaa
     1981 agatataatt ttagctatta ctacacttac tttaaatacg attaaacttg caatacttaa
     2041 atacattatt actcccacaa atggtactat aattaaattt cctataattg aaagcaatgg
     2101 tataccttta aatgtataat aaattatagg taaagttaat atatttgatg atattgtcaa
     2161 tgataccata cttaatttta taatactatt tattttatta tagaatatta atatagaaag
     2221 tgtagctaaa aaagacaatt gaaaacttac attatatata acataggggt tattcataat
     2281 taaaattatc ccaattagag acaatgccga tattccatct ttctttctat caataaatat
     2341 tgataaataa aaaatcataa caaatgctat agacctaatt atggatggag acgcacctac
     2401 cattatacaa tataaagtta taaaaatcga cagtattaat aatttataca atttatttat
     2461 acctcttaat agaaaggata taattacaca taatattcct gtatgaagac ctgatataga
     2521 tattacatga ctagttccag ttctggtaaa aatttctttt tgttcctcag tcaaattttc
     2581 aacttctgct aatagtatag aatttataaa atctgagttt ttcttatata agtacctata
     2641 agttttacta atataaaact ttatttcccc tattaaagag taaagtttat ttttatcaat
     2701 aattttatag ttatttatat acactatacc tttatatcca gtacttttta tataccttcc
     2761 ataatcaaaa tcatcatact tcattttatc taaacttttg aatttacctg taatattaac
     2821 tttctgacca atcttcaaat ttttattttt tgtataatca tttattagaa aaacccctac
     2881 cttgtactga ttgtatcttt tcttttcaat ttttcctttg acaactcctt gtatagttat
     2941 tatcttatca ttaagttctt cgtcgttttt tgtgttattt gtaatgataa atgatatag
//
LOCUS       DAKBNN010000304         2990 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000304 DAKBNN010000000
VERSION     DAKBNN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.310"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..942)
                     /locus_tag="OUB02_003711"
     CDS             complement(<1..942)
                     /locus_tag="OUB02_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCT8887428.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKI"
     gene            complement(911..1357)
                     /gene="lspA"
                     /locus_tag="OUB02_003712"
     CDS             complement(911..1357)
                     /gene="lspA"
                     /locus_tag="OUB02_003712"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCT8887429.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1664..2443)
                     /locus_tag="OUB02_003713"
     CDS             complement(1664..2443)
                     /locus_tag="OUB02_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCT8887430.1"
                     /translation="MKKIIWGICICLLSIVGVYTYSHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDGGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2498..2980)
                     /locus_tag="OUB02_003714"
     CDS             complement(2498..2980)
                     /locus_tag="OUB02_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HCT8887431.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCANKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
BASE COUNT          960 a          515 c          281 g         1234 t
ORIGIN      
        1 tatcttttta ataacttcct taaaatactc aggtaattct gaattaaatt cgatatattc
       61 cttagtagta ggatgtataa atcccaactt cttagcgtgc aaagtttgtc cttttaattt
      121 aaatttagtt tcttttggac catatacata atcacctaaa agtgggtgat ttatagacaa
      181 agcatgaact cttatttgat gagttctacc agtttcaagc ctagctctca tatgtgtaaa
      241 cttatcaaat ctttctacaa cttcaaagtg agtgattgca ttttttccat cttttactac
      301 agccattttt aatctatcct taggatttct ccctataggt ttatcaacag ttattttatc
      361 ttctttaacc acaccatagc atataaattc atactcccta gttatagtat gttcctttaa
      421 ctgttctgct agaaaattat gtgcataatt gttcttagct atcataagaa gaccagaagt
      481 atctttgtct attctatgaa ctataccagg tctaataacg ccatttattg atgacaactt
      541 atctttacaa tgatataaaa tcccgtttac taacgtattt tcatagtttc caggagcagg
      601 atggacaacc atatcctgag gtttatttat tattaagaca tcatcatctt catatacaat
      661 atctattgga atatcttgag gtaaaacttc taatacttta ggttctggta tctctataac
      721 aatattatct tcctctttaa ccagatatct agctttttct actttattat taacctttac
      781 ttttccatct tttataattt tttgtatata acttcttgac atatcaccta attgttctgc
      841 tagataaata tcaagtctaa cttcttcttc ctcttcaagt actaaaaact cttttacttc
      901 atccattata tcacctactt ttactttcaa aaaataaaac atatatacat aaaaacacag
      961 ttcctacaac tacaaataca tcagctatat tgaatacata ttcccatata attctaaagt
     1021 cgaagtaatc tactacaaaa cctaatcgta ctctatcaat tagatttccc aatgcaccag
     1081 atataattaa tataattccc aaccttccaa atatatgtac tttccttgta ttaagatagt
     1141 atagtccaaa tactgttgca agtaatgcaa caattataaa tatccattga ttattttgta
     1201 ataatccaaa tgctgcacct ctattttcga cataagttaa atgaaataca ttatttatta
     1261 ttggtattgt tgatacatcc accaaattat tcaataccca tattttagac agttggtcta
     1321 aacctatgag tagaattatt attaatatat atagcaatga tttttccccc tataaattat
     1381 ttaaaaacca attttatttg aagcacacta ttattataca aaaaaaatgc tctaaagagc
     1441 aactttatta aaattgtatt ttcttaataa agctagaaca aaatttaaaa tagtactact
     1501 ttttttatat tgtagatttc tacatattta aaatcatatc attatgtata aaaaatttaa
     1561 aaataccata ctaatcaata ttttttgtgt attttataga ttaaagatta atgatgtctg
     1621 taatttaaac ttacaatcac cactaatctt caaacaaata tttttacttt tgcacttgct
     1681 tatttttatc taaatgttgt ataagttcag atactgttac gaatttatat cctctctctt
     1741 ttaatttagg aattactact tctaaagctt gtatagtttg tgatttatca tttcttttag
     1801 tgttataatc atgtaaaagt attatattac cattttggac ctttgtaagg atagtattta
     1861 taattgcatc tactccaggg ttagaccaat ctctagggtc taagtttgac caaagaatta
     1921 tattcatgcc tttagaaact ataatatcac agacactttt actcatggct ctatatggag
     1981 gtctaaatat ctttggttct actcctatta cactttttat aatattttgc gtatctgtta
     2041 tttctttttc aacactacta taactatttt ttgcaacatt tatatgtgta aatgtatgat
     2101 tccctatttc atgcccccca tcatattgtc tttttaataa ttctttgtta tattctactt
     2161 tttctccaac tacaaaaaaa gttgctttta catcattttt ctttaatata tcaagtactt
     2221 gtggtgtaaa atcctcatct ggaccatcat caaaagtcaa tgctaaaatt ttttcatcct
     2281 catttccttt ttttattatt acatcttcat aatcgtcttt tatattgtca tttaaactat
     2341 atgcttcttt tgaactatct tgattatgtg tattatgact atatgtatat acacctacta
     2401 tagataataa acatatacat ataccccata ttattttctt catgtcatta cctcttttct
     2461 aattcattct agcaatatat tttataatgt cagtttttta atctctcaat attccatttt
     2521 tatttaaatc agttaaattt gttaggtctt ctgaataaaa gttagaatct tctacatcag
     2581 ggttttgcat tgcatcacct aataaattat tttctttatt tgcacaatca ttacataaac
     2641 ttgtatctgg caatatatcc aatctttcta aatctatttg attatgacat aaatcacaaa
     2701 ttccatatgt tccattctct attttagata atgctaagtc tatttgatac aatcttccct
     2761 cttcatgatt tattagactg ttttgcatat tttgcatata taaatctgtt cccatgtctc
     2821 ctatatgatt atcataacta gataattctc ctgatgaata cttttcactt gtatgtttag
     2881 ttgtatctcc aaataaagta ttatctttca tatcctctac taaattagtt aaattttctc
     2941 tttctttcaa taatttatct ttatattttt tagtatccat ttttaacacc
//
LOCUS       DAKBNN010000305         2967 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000305 DAKBNN010000000
VERSION     DAKBNN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.311"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            137..505
                     /locus_tag="OUB02_003715"
     CDS             137..505
                     /locus_tag="OUB02_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT8887432.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            508..879
                     /locus_tag="OUB02_003716"
     CDS             508..879
                     /locus_tag="OUB02_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HCT8887433.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1023..1478)
                     /locus_tag="OUB02_003717"
     CDS             complement(1023..1478)
                     /locus_tag="OUB02_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887434.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1517..2782)
                     /locus_tag="OUB02_003718"
     CDS             complement(1517..2782)
                     /locus_tag="OUB02_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HCT8887435.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1098 a          411 c          412 g         1046 t
ORIGIN      
        1 aataaaactc aaagttatat tagtaaaaat tggaattatt tataatatac tccaaaattt
       61 aaaaataatg gttgaaaaat ctaattaaga gtactataat ttaattaagt aattacttaa
      121 tcaaaacgag gtacttatgg aaaacttggt taaaatattt aaagcattga gtgatgaaac
      181 tagacttaac atacttatat tagtatccaa gagaaatata tgtcaaaagg ggatttctaa
      241 atatctaggg atttcagatt ctgctgtttc acaacatata aaaatattaa aagatgttgg
      301 gataataaca ggatataaag aaggatacta tgttttatat catattaata aagaatcatt
      361 taatatatgt gtagatttta taaattctat gttaagtaat tctagtgaaa gctttataga
      421 tgtatttgat gtaaatacaa tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa
      481 atgttgtaag agaagggaga aataatgatg aaagtatgta taccagtaga ggagaataaa
      541 ggattggata gtaaaccata tggacatttt ggttctgctc caatttttgt tgtgtgtgat
      601 ttagaaagtg gagaagttaa atctttagat aatggagatt tagatcatga acatggaaaa
      661 tgtcaaccat taaaagcatt gtcagggaca gctgtagatg cagtagttgt tggaggaata
      721 ggacaaggag ctataattaa attaaatgga atgggaataa aagtttacag agcagaaggt
      781 gatactataa gtgctaattt agacttactt aaaaatggaa aattagtaga atttccatca
      841 gaccatactt gctcacatga cggatgtggg catcattaaa aaaaacagct gatgaaatat
      901 atacttcatc agctattttt tcttttaata aatacgattt tattctttta aaaccaaaat
      961 ttagattttt atgttttttg tagtatcctt aataatattt caagtttaca agtttactac
     1021 ttttagattt ttttggtatg tttttctata tagtcaatat catttaatat gtcattgagt
     1081 tctttattaa gtgtttttaa atcatcacca tctacattgt agtttaaaaa tagttcctct
     1141 acattatcaa tgatagaatt gacctttcta gtaacttcaa caaatgtctt aaagttacta
     1201 ctatttttag catttagaaa tttttttaaa tcctttgata taaatgatac tatttcatat
     1261 tcatcattaa atagacctct accaatagga tatggttcaa ttctctgtaa aaaatattgg
     1321 tctttagcat taatattata agtataagat atttcccaac cgtctcctaa atcaaacttg
     1381 ataaaatata atgtactact tacttttgat attcttgtgg caccaagctg tcttaaaaga
     1441 acttcaatcg aatcaccttg aacagtatta atattcatat atacacatcc cctttagtat
     1501 tattatacta tattttttaa aattttataa ttaaataaac agtagatata ataatggata
     1561 taatcataag tggaaaacct attttgaaat actccttaaa agagatagga tgaccgtgtt
     1621 tattacctat acctgctagt acaacattag cagatgctcc tattagtgtt ccatttcctc
     1681 caagacaagc tccaagagat gttgcccacc atagtggcat tacatcaata ccttcagctt
     1741 gcatagtcaa aataagagga attagtgttg caacaaatgg aatattatct aagaaagaag
     1801 agactatagc tgacaaccat aatatcaaca acattgtaaa tacaagatgt ccttctgtaa
     1861 gtccaatcaa tgcttgagcc aatttgttta ttatcccaac ttctactaat ccaccaacta
     1921 ctacaaataa tcccataaag aataaaattg tagaccattc aatacctgcc ataatttcat
     1981 ctacatcttg ttttcctatt aaaagcatta tagatgctcc tgtcagtgct acaacagaag
     2041 aatctatgtg gattgtactg tgaaacataa aaccaaaaag tatgaatgct attaatataa
     2101 ggctttttat aagtagaggt ttatctttaa cagatttttt ttcatctagt tttaatattg
     2161 catttttagc tctttcattc acaacaagtt cttttccata aataaatcta aagcatatta
     2221 tagttatagc aagtattact ataacagcag gacctaaatt tattacaaaa tccataaaac
     2281 taagattggc agcactacca atcataatat ttggagggtc tcctataaga gtagctgttc
     2341 ctcctatatt tgaagctaaa atctgtgtta ttaaaaatgg aactggatta agtcctaata
     2401 tttgtgtaat aactatagtc attggtccaa taagtaagac ggttgtaaca ttatctaata
     2461 cagctgataa aatagctgtt ataatagcaa agcaaatcat tatcttccat gggtcacctt
     2521 tggctttctt tgcagtccag atagctatat attcaaataa ccccgagttt ttgacaacag
     2581 ctacaaatag catcatacca actaatacac caatagtatt gaaatctata tggcttaatc
     2641 cttcatcaag tgtaagtata ttgaatatta tcattaatag tgcaccacca acagctgcta
     2701 cggatctgtt taccttttct gtaataatag ctgccataac tattaaaaat atacatatag
     2761 ctattatttg ttgaaattcc ataatataag tacctccatt tgtattattt tgtgcctaat
     2821 gctagtacta tatatagtat tactttttta ttgttttttc aatggatgta tgaaagtata
     2881 ttatttaaca aagtttttga aaaattgaca gaatttaaag aaaatttttg ttatttaaat
     2941 aaattttaaa attattccat tgataat
//
LOCUS       DAKBNN010000306         2967 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000306 DAKBNN010000000
VERSION     DAKBNN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.312"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            113..1006
                     /locus_tag="OUB02_003719"
     CDS             113..1006
                     /locus_tag="OUB02_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCT8887436.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1449..1939
                     /locus_tag="OUB02_003720"
                     /pseudo
     CDS             1449..1939
                     /locus_tag="OUB02_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1967..2914
                     /locus_tag="OUB02_003721"
     CDS             1967..2914
                     /locus_tag="OUB02_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887437.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQKGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         1302 a          257 c          419 g          989 t
ORIGIN      
        1 taattaaata tatttcataa atcttcataa aatcttcaaa atttcgtaaa gaatatgtca
       61 caaaagtaag gtatattata ttcagcagat agtataaggg aggacaatat ttatgaagaa
      121 taaaaaattc attgttataa tactgataat atcaatttta ataggtgtat tagtgaagga
      181 atattcatct agagagataa aaaaggatga tataaatgtc agtaagtata taaaatatgc
      241 tgatttagca agtaaaaata atgcacaagt taactggaag tatgtagctt ccattgttgc
      301 tgtgttaaat aaaaataact taaaaaacgt taaggattca cagatacaag aagtatcaga
      361 tttatttgtt aagaattttt ctaaaaataa taaaataaat aagttaagtg atattttgga
      421 tgagttagaa tttagtaata gacaaaaaag attggttgat aattatatag acaatcttaa
      481 ggattatggt ataaaaccag aaagattgaa gtctgataca aaatatatga agtttattga
      541 tgaaattaaa acagaagcaa tacaaaatta taaggattat aaaatacttc catctataac
      601 tattgcacaa gctataatag aatctagctg gggcaagtca actttggcaa aacaatataa
      661 caatctattt ggtataaagg ctgacgctta ttggaaaggt aagtcagtga ctttagaaac
      721 gaaagaacat ttagatacaa ttatagatga taaatttaga atatatgatg ataaaaatga
      781 atctataaaa gaccatgcaa aatttttagc cacaaataaa aggtataaaa acaatggtgt
      841 atttgatgca aaaacgtata tatatcaagc taaagcatta gaaaaggcag gttatagtac
      901 tgcgaaggat gaaaatggca atagtatata tgcagctcgt ctaatagaat taattcaaca
      961 gtataatctt caattaatag acagtgaaat tcaaagtgaa atgtaacttt gattaaattt
     1021 ttattgataa ggctatgatt tttaaaatca tagccttatt ttgacatgtt tttactttta
     1081 attattaaat cgatatcatt atctttcaaa atttacttta tactgtaaat taaattattt
     1141 aagttaaaac ttggaattat ttataaaagg attatttact ttatctaaat tctgcactta
     1201 aagaacatct tttcccttaa gactgaccgt tagtctataa aaattataat tacttataaa
     1261 aaagtgaata gagagatata tatataattt tcagaaagac ttttacttgt aaattgaaat
     1321 taatgaagta aaaaaggatt ctttcttaaa agtaaataac aactagatag tgaataataa
     1381 aaaatggtaa tttgaaataa tttttatgaa tagtattaat atatagatac agggagattg
     1441 atgcgattat gtatgaaaat aataaggtaa tatacaacct tgaaagatgt gaaaaactag
     1501 aaaagactct ttatttatct caatttaata catataaaaa ttttaaatta gcttctaaaa
     1561 aaactgtaat aaattcatta gagacatatg tttacaatat gaatattaac aatataagtc
     1621 ttgttgatac tatagaatgt atatcattag aagatgaaca acttacagga aagaagttga
     1681 ttttagttgg tgatatagat attgatgcaa tattagatta tgcaggaaat aaaagaaata
     1741 gaaattctag taaaaaaata catttaaatt aaaaataccc tttagtacat ttatacaaat
     1801 gcctagaaag atagaaaata aagataaaat taatttaaag tatcttatac aagatattac
     1861 ttcatctatt ttagatgata atttatttat tagtgttact gcaattataa gttatgaaaa
     1921 tagttccaag atagagtaat ctttaaatta tagaaaggtg gatgcaatga gaataacgaa
     1981 aactactttt aatgatgtat tttcaaatga tgcaaatgga agtattataa aagtagaaaa
     2041 atatgttgat aaaaaatgtg catctgtatt tgagattttg acctacacta ttattgttac
     2101 aaatacaagt aggtacaaaa ctggaaatat atttttcaaa gattatattt ctaaatatat
     2161 agagtttata aataatactg taaaagttaa tggcattata aaacgtggat tagacccaca
     2221 gaaaggtttt tacataggga gaatagatgc aagttgtaaa aaaataatat cttttaagag
     2281 tgtagtttta ccaaattctg cacataggat tataaagaat agtgctaata tatactatta
     2341 ttataagtgt aatctagata aattcccaac aagaatatca atagaaagta atagagttta
     2401 tactaatgta aataaaattg tattcaaaca attgaatata agtaatactt taaaagctcc
     2461 taaaaatcta agagatatat taaaagtaaa tgtaaatagt aaaatatttg atgttaaacc
     2521 tatgaaaaat cttataaaca aaggtagtaa tttgaaatta tgtaacttga tagtttttgg
     2581 gtgtatagaa gttgaaatag actatagttg taaaaatagg agtaatttta aaacagtaaa
     2641 taagtctaat gacattaaaa aagataagta ttataaaaat gatatacaaa ataacaatga
     2701 aaatataaac gattgtaaaa agcaaataag ttttggtgat aataaggtaa aaaaaataac
     2761 aaaaactttt ggattttctt gtttcatttg ttctccagtt ggaattattt atgaagatat
     2821 gaaaaatata aatataaaaa ttgaacatac atcaataaat gaattaaatc cagaagaatt
     2881 atttgtaaat acatcacttt tattgtatta ttaagtattt taaagctaaa acttaaactt
     2941 attaaaatag catttggtta aaggaaa
//
LOCUS       DAKBNN010000307         2887 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000307 DAKBNN010000000
VERSION     DAKBNN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.313"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            64..402
                     /locus_tag="OUB02_003722"
     CDS             64..402
                     /locus_tag="OUB02_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887438.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            424..741
                     /locus_tag="OUB02_003723"
     CDS             424..741
                     /locus_tag="OUB02_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887439.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            891..1568
                     /locus_tag="OUB02_003724"
     CDS             891..1568
                     /locus_tag="OUB02_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887440.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1896..2663)
                     /locus_tag="OUB02_003725"
     CDS             complement(1896..2663)
                     /locus_tag="OUB02_003725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCT8887441.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         1156 a          379 c          341 g         1011 t
ORIGIN      
        1 gtataaatag tctatataaa acaacttaaa ctatgaatat aatctaaagt ggagggataa
       61 taattggcaa atataggaaa aataatagga gatagaataa aagtctatag aacaaaattg
      121 ggatatagtc aagagtttct agcagaaaag gcaggtttac atccaacata tattggtcag
      181 attgaaagag ctgagacaaa tacatctatc aatattataa tgaaaattgc tactgcttta
      241 gacataccac ttgaattgtt atttgcaaat attattacaa cagaaggcgt tgataactct
      301 gtacctttag aatgttatga aatgattaat aaattaactg aaaaagaaca aattgcaatg
      361 ctagagttaa taaagtgcat cattaaatac aaagaaatat aagtgaggga aatagtaaca
      421 ataatgaatt atataggtaa aagacttaaa gaagaacgga aaaaagcaaa tttaacttca
      481 aaagaatttg caaatatggt tggagtatcg ccttggtata taactcaaat cgaaagtggt
      541 aaaaagaatc caagtcttaa aacattcata aaatttgtaa atatattaaa tatttctgca
      601 gatgttctta taaaagatat tacttccact ggaaaaacat atttagaaaa tgatataaat
      661 gaagagttaa aagacttaaa ttcaagagaa ctaaatctga tatcacagat tatagacatc
      721 ataaaaaaga ataaaatata attctttata ttttatataa aaaaaaaggt tatttgaaat
      781 aaataacctt tttttgtctt tttgtgttaa cattgtctta tagtgctaat gagtactatt
      841 agaccatgtt ctatggcctt attttaatat aaaacgattg gagataggct atgattgtga
      901 ttgaaggtag cgataaattt aagattgcaa aagaatatat tgatgtagaa tatactcttt
      961 ttagcaaagt aacttttagg tatgaaaagt tgaaatttaa agataatgct gaattggaaa
     1021 aaattaagat gtttaaatat aaaaatggct acatccctaa taaattaaac ctttcttttg
     1081 gatatggatt ctcttcttat aaaaagcaaa taattagaga aactgtagat actttaagat
     1141 tgacagaaat tttttcaagc gagaacatag aagatattaa atttataaaa gatggtacaa
     1201 aaaaattaga aattagcata gagaaagttg tgaaatttaa acgtcgaaaa aaaagaaatt
     1261 atgtttgttg ctattgccct gatatgtata gagacataaa actcgacaaa gaatctatca
     1321 ataagatata caacagaaaa ataaaaatag aaagagaagt taatattttt gaagatgagg
     1381 atgttataat aaacaagaaa gtattgaagt ttccaaagtc ttggacaaag aatatgcaaa
     1441 aatattggtt aagtgaaaat aagtatccca tacattctac tgtaattgat gatgatagat
     1501 ataaatgttg taatgtacaa tatacaaaaa atagagtgat aatattatat tacatatata
     1561 accattaata tacataatta caaaaaaata atgacaaata tacataatac aaatatatct
     1621 tctactttag ataaaacagt tattatgaaa acttactatc tataaataat agaaatgcta
     1681 gtataccatc tataatctaa tgtctagtag gttttaatgg catattttaa atatgccata
     1741 gttgatttat aagattttct atatcacttt caaaatacac actaaatagt tgcttttcag
     1801 atataattgc ccaatttaaa ttttactatc tgaaaagttt tttatattta atttatatct
     1861 tcaaaaatac cgtctaatat ttctatataa ttaatttaat taatactctt atgtaataga
     1921 ttaagtactg aatttaactc tttaaatgat atttgccctg gtgctgaaac actttttgca
     1981 gctccaaaag tcaatgcaga accaaatatt tcaccacaca atctacttat aacacccatt
     2041 cctgacattg acattgttat tattggtcta tctgcataaa ttttaaacat ttcatttgta
     2101 gcttcaagta gcactaacac atctttttca ttttgcggca taacagctat tttgggtaaa
     2161 tctgcgccca attcctgcat tctacataaa cgagatacaa tctcttcttt ttttggagtt
     2221 ttattaaaat catgattaga tataattact ttcacttctt ttttatgagc aaaattaact
     2281 acttcatcta tgacctcatc tcccataaat aactctacat ctattaaatc tactaaacct
     2341 gtattagaaa tttctttatt caaagtagta taataatctc ttgatattaa tttttctccc
     2401 ccttctacta cacttctaaa tgtgaataat aaagggatat catgtatata acttcttaat
     2461 tcatagagaa cttccttaac ttctttaata ttttctacat tctcaaaaaa atcaactcgc
     2521 cactctataa tatccaaaca tgcatctttt aactctttag cctcttttat aatatctttt
     2581 ttatttttcc caataattgg tacacaaatt ttaggtctac cttctccaat agtaatattt
     2641 tttacttgaa cttttctttt catattctcc tccataatca gaaattctat aggactttaa
     2701 attctatttt ctgcacaatt taattataca ttaaaaatac tatacattat aaaaatacta
     2761 atttaaaaat aatttttatt ctaattctta aaacaatccc acatatttta gaaaaggtac
     2821 tctataatat acaaacactt cttttttacc taaatttatt ttaacatcct ataattttca
     2881 taatatt
//
LOCUS       DAKBNN010000308         2866 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000308 DAKBNN010000000
VERSION     DAKBNN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.314"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     misc_binding    114..345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            453..1934
                     /locus_tag="OUB02_003726"
     CDS             453..1934
                     /locus_tag="OUB02_003726"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCT8887442.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1944..2837
                     /locus_tag="OUB02_003727"
     CDS             1944..2837
                     /locus_tag="OUB02_003727"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCT8887443.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
BASE COUNT         1111 a          331 c          451 g          973 t
ORIGIN      
        1 tcaatttgat tacaattttt ttacaattta aaaaatattt tttccaaatt tttatattaa
       61 tacttgactt tgtctgtttt ataaactaga ataattaata atattaagaa tattgaaagg
      121 cattgattag gaagagtaaa tctgaatcga ttaccagaga aggaaactac tagctgaaat
      181 gtttccctaa ttagaaggat tgaaaactac ctaggagctt ctatccgaag ttatcttaat
      241 gattttagta ggaatagacg tttttattac ttgcgttaaa gtatcaagtg gattttttaa
      301 aatcaatttg ggtggaaccg cgggaaacag attctcgtcc cttttttagg gatgagggtc
      361 tttttttatt acctaaattc agtttttaaa atattctgta tctaatttaa ttagctaatt
      421 aaaaatttaa aatatatgga ggattataaa aaatgtatta ttcaagtacg agaggcaccg
      481 aagaaaaagt aactgcatca caagcaataa taaaaggaat atcaagtgat ggtggtcttt
      541 atgttccaag tgaatttccc aatgtaaaaa atgaacttat aaatttagta aatttaacgt
      601 attctcagat agcatttttt gtacttagta aatttttatg tgattttact gaagatgaaa
      661 ttaaaaattg tatagaaaat gcatatgatg aaaagtttga ttgtagtagt atagctccat
      721 taaataaagt aaatgatact tatttcttag aactatatca cggtccaact ttagcattta
      781 aagatatggc acttactata atgcctcatc ttcttaaaac atctataaag aaagataatt
      841 tagagaaaga tgttgtgatt cttactgcta catctggaga tacaggtaaa gctgcacttg
      901 agggatttaa agatatagat aaaataaaga taattgtatt cttcccagaa gatggtgtaa
      961 gcccagtaca aaaacttcaa atgaaaactc aaactggaaa aaatacatat gtagttggta
     1021 taaaaggaaa ttttgatgat gcacaatcag gtgtaaagaa gattttttct gataaagaat
     1081 ttaatcaaaa actattggat aataattata tattatcttc tgcaaattca attaatatag
     1141 gaagacttct tccacaagtt gcatactatt tttattcata tatgaaatta gttagcaata
     1201 gtgagataaa tttaaatgat aaaataaatt ttgttgtccc aactggtaat tttggtaata
     1261 ttctagctgg atattatgcc aaacaaatgg gtcttccaat aaacaaattg atatgtgcat
     1321 caaatgataa taatgttctt tatgatttct ttaaaactgg tgtatatgat aaaaatagaa
     1381 cattgaaatt aactacttct ccttctatgg atatattaat ttcaagtaat ttagaacgat
     1441 tactgtttga aatatcaaat agaaatactg atattgtaaa taagctttta tcagacctta
     1501 gtgataaagg agtctacaaa ataagtgatg atatggaaaa aaatttagcc tcattctatg
     1561 gagaatattc aaatgaagaa gaagttaaaa gcacgataaa caatgtgttt aaaaattata
     1621 attatttgat agatactcat actgctgttg catacaattg ttatgaaaaa tataaaaaag
     1681 aaacttctga taacacaaaa actgttattg taagtactgc aagtccattc aagttttctg
     1741 aagatgttct aaagtctata gattgtgatt ttaaagacct tgatgacttt gctataattg
     1801 atagattatc aagtatttct aaaatagata ttccaaaacc tattaaaaat cttaaaaatg
     1861 cagaaatact acataaagat atttatgaaa aagatgaatt aaaactagct ataaaaaaat
     1921 tcttaaaggt ataggtgatt attatgttag agattatagt tcctgcaaca agtgctaata
     1981 taggtccagg atttgattgt cttggaattg cgttaaatat atacaataaa ttttatgttg
     2041 aagaaattga aagtggtctt gaaatagaag gttgtgaaga tgcttacaaa aacgaaaaca
     2101 acttagttta tacatctatg aagtactttt ttgatagagt taaaccagaa aaaatacctg
     2161 ctggtatcaa aataaaaatc caaagtgaag tacctatatg tagaggtctt ggcagtagtg
     2221 cctcttgtat agtagctgga gttatagctg caaatgctct atcaggtgct aatttagata
     2281 aaaatcaatt actaaatata gcttcagaaa tagaaggtca cccagataat gttgctcctg
     2341 ctattttagg aaatatgatt gtatctgtta ctgataatga gaacatacat tatgatatta
     2401 taaaaattcc tgaagaactt aaattttgtg caatgatacc taattttaag ttatcaacag
     2461 aaaaagctag aggtgtttta ccaaaggaaa taccatattc tgatggggta ttcaatgtta
     2521 gtagagttgc tttactaata tctgccttac taaacaaaaa ctttgaccta cttaaagtag
     2581 cttgtcaaga caaattgcat caagattata gaggaactct aattgagaac tacaatgata
     2641 ttgttgaaaa atctgaacaa ttaaatagta ttggagtatt tttaagtgga gctggaccta
     2701 ctataatgtc tttaataaaa gaaaatgatg atagttttgt ggataatatg aaaaattacc
     2761 tacaaaaact aaaatctgat tgggaaataa aagagttatg ttgcgattct aatggagctg
     2821 ttttaaatat aatataatta tcgtcttcta tacaaaatat aactac
//
LOCUS       DAKBNN010000309         2844 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000309 DAKBNN010000000
VERSION     DAKBNN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.315"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            444..1265
                     /locus_tag="OUB02_003728"
     CDS             444..1265
                     /locus_tag="OUB02_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HCT8887444.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDTDVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(1575..2354)
                     /locus_tag="OUB02_003729"
     CDS             complement(1575..2354)
                     /locus_tag="OUB02_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCT8887445.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         1062 a          370 c          341 g         1071 t
ORIGIN      
        1 aatataaatt agttttttta acaaaacaat aaaagagtaa agtacaaata attcctttgt
       61 gctctactct ttttaacttt ctttagacaa tgatataatg ataaatataa aaaattatat
      121 attccattct atagaaatat gctcaccaat aacatgtatg ttgttaatca atatattata
      181 tcaaattata tcaaaattac ataaacctta taggacaatc atatcttatt acaagaaaaa
      241 tgtcttatcc atattgaata agtcattcag ttcatataca aagtcaattt cattgagaaa
      301 catttaatat atgtttataa aaatttcata ctattctttc atatttgcat gtgatataat
      361 ttattaactc ataattttgt taatcagttg tgcttgtata ttatttatat aatctaatat
      421 tttaagaagg tggtgtaata actttgaaat taaaaattgt tggtagtggc ggtatgtcaa
      481 ttattccaag ttcattttgc aaatgcaaaa tatgtgaaga agccagacaa aaaggcggaa
      541 gatatgaacg attagggcca agtttatata ttgatgatat aaaaatgctg atagacacac
      601 ctgaagatat tgcagtagct tgtaacagac aaaaaatatc ggaagtaaaa catatttcaa
      661 tttctcacca tgacccagac catgttaagg gtattagaat tgtcgaaaaa ataggatgtg
      721 actttatcac aggagaaaat aatccaattg gcttttatgc gcttcctgaa gtaattgaag
      781 atataaaccg aacgaatctc gattgtctta aatattatgg tgatgttata aattgcattt
      841 ctattaatga aacttcacat gtaaagataa ataattttga tattgatttg attaacaata
      901 atccagacag gaatataacc ttctatgtta tcaaagagaa tgaaaaaaaa gtcatatatg
      961 cctgctgtaa tcccaagcca tttactcata gtgatatgta ctttgatact gatgttttaa
     1021 taataagtct agtttctgat gatggtatat taaatgatgg tacaaaactt aaagacacac
     1081 catttaaaga tgaaatattt actttagatg agattgttga aattaaaaac tattatagaa
     1141 ttaaaaagat tatcattaca catattgacg aaatgtgggg aaaatcatat ggttattaca
     1201 gtgaattaga aaagaaactt gataacattt attttgcata tgatggtatg gaaattatag
     1261 tctaaatcat taagtaataa gcataatgca aaataagaaa acttcaattt aacagtatag
     1321 ttaaactatg taatatcaac atatctcagt ttttacaaaa tatcatagta tttatcttta
     1381 taaaatttca agtcctacat attttaatac ttcaatatct aaataaagta aaatcaaaaa
     1441 tatattttat atacattata gacggcttat cggcactaga taagccgttt tgtcacaatt
     1501 attatttgct caacaaaact tctcataata tataacagat tgaatctcca actgatactt
     1561 aattaccgaa actcttattc cttaatatgt tgttgcatta accaaacctt catcatcatt
     1621 ttttgtggca atagttttgc taataaatga ctggattttg tatttatacc atacacagaa
     1681 atatccttat ttttctttgc atccttcaat gcttttttgg caacaatgtc tggagacact
     1741 atacctggga aattttttgt ccctttttca gctacaacta tacctctctt aaatagattt
     1801 gtactcatcc aacctggaca tacagcaatt gcatttatcc ctttactctt aagttcaaca
     1861 tttaatgctc ttgtataatt tcttataaac gctttagttg aactataaat attttgatat
     1921 ggcaatggct ggaatgatgc ttgtgaagaa atattcatga tatggcttcc cttttgcata
     1981 tatggaatac atataagtcc caatgctaca acagcactaa tatttaaatc aatcatatta
     2041 atagattcat ctatacttaa atcattgtat gaacagaatt ttgcaaaacc agcattgttt
     2101 ataaggtatt ttatacatac attttcttta cttaaaaatg taccaatttc ttttacttct
     2161 tctatttttg ataaatcttt tgaaaaaatt tttaccttac ttccaaattc atctactaat
     2221 tgatttaatc tcgccttgtt tcttgcaact gcccatattt ctgttatttc ttcttcattt
     2281 attaataact ttagaaactc tttaccaaaa ccactattag cacccgttat aattgcaatc
     2341 ttcttataac tcataattat tctcctttac cataagttta tacgctcaaa ctgtaaactt
     2401 attttttaca aataaaacac cctctattca gatgttatat tcatattatt tgagatatta
     2461 tatttatatc attttcaata attgttatat agcataaaaa atagtgttaa tattatagaa
     2521 tcggtattga ttatatttaa aaagtagtgt taattatatt atagaatcag tatttattat
     2581 attataaaaa gtagtgttaa tattatagaa tcgatattga ttatatttaa aaagtagtgt
     2641 taattatatt gtagaatcag tattgattat attgtaaaaa gtagtattaa taatattgta
     2701 gaatcaatat tgattatatt taaaaaagta gtgttaatta tattatggaa tcaatattga
     2761 ttatatttaa aaaagtagtg ttgattatat tataaaaagt agtattaata atattgtaga
     2821 atcagtattg attatattta aaaa
//
LOCUS       DAKBNN010000310         2816 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000310 DAKBNN010000000
VERSION     DAKBNN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.316"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     regulatory      307..403
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            457..633
                     /locus_tag="OUB02_003730"
     CDS             457..633
                     /locus_tag="OUB02_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887446.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(820..1059)
                     /locus_tag="OUB02_003731"
     CDS             complement(820..1059)
                     /locus_tag="OUB02_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887447.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1204..2013)
                     /locus_tag="OUB02_003732"
     CDS             complement(1204..2013)
                     /locus_tag="OUB02_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCT8887448.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         1021 a          394 c          247 g         1154 t
ORIGIN      
        1 atataaataa ttacgggatg agtctatata aaattcatcc cttgtttttt gccattcata
       61 tatatttttt caagatattt ttgttcatct attatctagc caagtaaccc ttgtgaacta
      121 aactttttaa tataaaaact tcaatacaaa actaaatata atttttatta aacattctat
      181 acatatagtt tgtttcttga gtgaaaaata gatttcatat tgttaagata ttgttaaata
      241 tttcaatttt ctgacaagta tgttgaacaa taacttatcc tagtgtatta ttttagttgt
      301 agaataaaac taaatcggca aaactagaga aatttagtga cgcaaagcta tagggactaa
      361 cgcttataaa ataaactatg agttatgtca gccagttgcc aaaaagatat tgttcttttt
      421 gttttatgaa agttttttag ggggatagta atatatatgt ttagagatga aatggataaa
      481 tgtacacaca tgttaactgc ttatattagt agtttatatg attattgtga ttttatagat
      541 acacagctag atgattttat actagagtac ggagaaaatg tagtagaatc ttgtttacat
      601 caagtgatgg tattggtaag taagtataat taaatagaaa ttgttttcta gagaaccctt
      661 tttagtttta tctacattta aaaatgttat tatattaaaa aactaatctc ccctatatgc
      721 aaagtttctt ccatacaaca atttcttccc atttatactt tttcacttgt atctttaata
      781 aaattcaact tcaaatatta atatacttca aaaattattc tatttcttat ctttacacca
      841 attcctatca gtattatcct ttatataggt tctaactatt tctccacaat ctaaacataa
      901 atctgaatat atagtagatt gtactggaaa agttttattt ataactttat ctaattttcc
      961 atctcctcta tattttaatc caatagtttc agcatatatg ccaccagttg ttaaaattcc
     1021 cttttcaata tttacacttc cacaattaga acatttcaca tttataaccc cctaaacaat
     1081 tattttttca tgctatctta taatttaaaa tataatttgc taccgatttt ataacacagt
     1141 gacaaatcgg tggcaaatca acattttttc attctattta ccatctaatc cattgaaata
     1201 tagctattta gttataggaa cttgaatttc cgttacaaac tcctctacaa ttccagtctc
     1261 atgtatatct atcttgtaga tttctatagg gtcagcaatt atcttataat tgttttcttc
     1321 tataaattta aacaccttct ccatatacat cttattatta gaattactcc cagtataatt
     1381 taaagttaca taatatcctt catcaaaaac tatattatat actttctcat tactttccag
     1441 aaaacaaaat acagatttaa actcattaaa tataccttta ttaattgctt cagtagaaaa
     1501 ggcagaacca atattgttat ttccaagtat attaaatcta tcttcatatt ctttctgaag
     1561 tttttgaatt aaaaaatcga agtcttcatc cctctttata tcagcattta attctaaagc
     1621 ttttcttttt tctatatata caacctctat ctcatctatt tttgtatttt gaattacact
     1681 ttcaattgaa ctcaatcttt ttataatatt ttccttatgg cttataaatt cagctatttt
     1741 tttatcaatt aaatcgagtt cttcatttaa aatatttttc gtagactcta tacttctgtc
     1801 atctaggtat tcttttattt tcttcattgg aaagttcaag cttctaaatt cttttattaa
     1861 atttaatttc catatatctg atatattata cattctatat ccatttatat ctctaactgg
     1921 tcttagaatg cctatttctt cataatacat taatgaatct ctacctattc catatatttt
     1981 agatatctct cctattttat agtaatcttt catttatata ataaactcct ccgaaatctt
     2041 atttttattt taaatcaaca ttattactct ataatactat agtattagaa tatactaaaa
     2101 aaatccttaa aacaaggata ggaaaataaa catcctaata attaattatt aaaatatttt
     2161 tatattgtaa tattaggtat agtatttata acttcaactt ttttataaat atattttagt
     2221 tttatatagt tttatgttta cagctattat atccatctct atgtaatatt tttcatatta
     2281 aaactgattt ctatagatta atagtttata aatttagtaa atgaatattt attatagtct
     2341 ttaacaaaaa atacaagaat aaacttaatt ttaataacta aaattaatta tgcaaaatta
     2401 ttaatatggt attaaattat gtataaaaag ttaattatga ataaaataat tatgcctttg
     2461 aaaaatcatt aaacttaaaa ttaattataa aaaaactcta ccttactaac tatacagctc
     2521 acaatctatc tatttatgaa aaaatatgat ataattgcat tcaaaaagga aatctttcaa
     2581 tatactagaa tttaagcatt aactaatttt atctaatatt attactaaac taaaaaatat
     2641 ttgtatttgt agcattggag caagtaactt aatgtatatt cattttaaat acttatttaa
     2701 aatttagcat ttaaattatt tacaaattaa catttaaata cttctagcat aaatacagtt
     2761 gtcatcaaaa tttaaagtat cttatggagg aataccatgc aaaatcataa ttatat
//
LOCUS       DAKBNN010000311         2805 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000311 DAKBNN010000000
VERSION     DAKBNN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.317"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            206..1027
                     /locus_tag="OUB02_003733"
     CDS             206..1027
                     /locus_tag="OUB02_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT8887449.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1106..1317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1404..2720
                     /gene="argH"
                     /locus_tag="OUB02_003734"
     CDS             1404..2720
                     /gene="argH"
                     /locus_tag="OUB02_003734"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCT8887450.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         1147 a          250 c          549 g          859 t
ORIGIN      
        1 cattataaga ctataaattg aaaatattca gaaaaatact acaagaaacg atttattttg
       61 ctcttaaaat aagttaaatg aatgttatta attttgtaaa ttgaaatagt ttaagtataa
      121 agttatgata taatctaata agggtaattc tttagattat attaaggaaa tataggtata
      181 caataaaaag ttatggagtt ggattatggg atataaatta attgtaacag atatggatgg
      241 aactttatta ggcaataatc ataaagttac agatgaaaat aaaactgcac tacaaaaagt
      301 tataaaaagc ggtattaatg taactttagc aacaggaaga gccttcgatt cagctaaatg
      361 caatgtagat tttcttaaag aggacatgcc aataatagct tgtaatggtt cattaataag
      421 agagcaaaat ggaaatataa tatattcaaa taagattgat acaagaacat gtttaaacat
      481 tttagatgta ttagataaat acgatattta ctaccaatgt aatagtatag attctatgct
      541 ttcgaaaaaa atagaaggac gtgaagatag attaagtgta tttttaggct ctgaaacaga
      601 agtaattgta aaagatgatt tgagagaaga aatctttaaa aaagatatac taaaatttgt
      661 aatcatagaa gaaaaaaatc catctatttt ggatgaaata agaaaagaat tgagaaaagt
      721 tcaaggaata aagataactt cttcatggcc aaacaatatt gaagtcatga atgaaggtgt
      781 tgataaaggt aatgcagtta aaattttagc tgaaaaaatg aatatagaca gagaagatat
      841 aattgcattt ggagacaatt acaatgacat agaaatgata aagtttgctg gtcttggggt
      901 agctatggga aatgcagaag aattgattaa acaagaagct gattatgtta cagatacaaa
      961 tcaagacagt ggagttgcaa aggcaattta taaattccta gagttaaaag aatcacttag
     1021 ctcttaaaac agtatataat aggaaaaaat aggttgacta ttttgtagat agtaattata
     1081 atacaaatag ataaaacata aaaatttaga ttcagtgaaa aagagagtag atatagagaa
     1141 aatctcagcg aattggggga tggtgtgagc ctaatgataa cactttatat tgaaaagaac
     1201 tttggagaat actacttgaa attcagtatg gtagtaggta atctcgcctt aagagaatag
     1261 agtgggtaaa cattgtttac caactagagt ggtaacacgg aatatttcgt ctcttggtct
     1321 ttttgaccaa gagatttttt agtttcattg agttacaaga cttataatta aaatacaaaa
     1381 aattaaaata tgaggtgaaa gttatgaaat tatggggcgg acgctttaga aaagcggaaa
     1441 atcaacttat ggaagaattt aataaatctt ttggctatga ttgtgtactg tacaaaaaag
     1501 atatcgaagg aagtgtagca catgttcata tgcaagtaaa atgtggactt ttaacagaag
     1561 aagaaggaaa gtctataaca gaaggtttaa aagggatact tgaagatgta gaaaatggaa
     1621 agttagtatt ggatggagag tatgaagata tacatagttt tactgagata aatttaattc
     1681 aaagaattgg agatgttggc aagaagcttc atacagcacg tagtagaaat gaccaagttg
     1741 cagttgatat gagattgtat gctaaagaaa aagcaaatga tttggtggat ttaatatctg
     1801 aatttaaagc tactataaaa gatgttgctg acaaaaaccc agtgatgatg ccaggctata
     1861 ctcatcttca aagggcacaa gtagtgacat ttaagcatca cttaatggca tattatagta
     1921 tgtttgatag agatgagaaa agaataaaaa atgctataga aatattagat gaatcacctt
     1981 taggctgtgg agctttagca ggaactacgc atgatataga tagaagtata acttgtgaac
     2041 aattaggatt taaaaaagta gtagataatt ttatggatgg agtaagtgat agagattatt
     2101 tactagagct aatgagtgat ttttcaatta taatgatgca cttaagtaga ttaagtgaag
     2161 agttgatact ttggagttct caagaatttg gatttgttga gatagatgat ttatatacaa
     2221 ctggaagtag tataatgcct cagaagaaaa atccagatgg tgcagagctt ataagaggta
     2281 agactggtag agtatatgga aacttatttg gtctttttac agtaatgaaa ggaataccat
     2341 tagcatataa taaagacatg caagaagata aagaaggttt tttcgatagt gtacatactc
     2401 ttgaaatgtg tatacaaata atggatagaa tgatagccac actaaaggta aatgaggata
     2461 aaatgaaaca agcagtaaaa aatggttttt taaatgcaac agaagttgcg gattatttgg
     2521 ttaaaaataa tgttgctttt agagatgcac atggtatagt aggaagtatc gttatatatt
     2581 gtgaagataa taaaaaagca atagaagatt taacattaga agaattacat aagttttcag
     2641 atgcttttaa ggaagatatt tatgatttta tcgactatga gagtatatta aataagggaa
     2701 taaaaaagaa tttaaaataa tgtatttatt tttaaaagct gtaaatttga atgttatcat
     2761 ttaaatttac agctttttat tgtgtaagat tttattatat aaaag
//
LOCUS       DAKBNN010000312         2803 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000312 DAKBNN010000000
VERSION     DAKBNN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.318"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(9..782)
                     /locus_tag="OUB02_003735"
     CDS             complement(9..782)
                     /locus_tag="OUB02_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887451.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFTS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(788..1675)
                     /locus_tag="OUB02_003736"
     CDS             complement(788..1675)
                     /locus_tag="OUB02_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887452.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDILSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(1689..2711)
                     /locus_tag="OUB02_003737"
     CDS             complement(1689..2711)
                     /locus_tag="OUB02_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887453.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFIAPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGTMSLCFLVIK
                     SDYISR"
BASE COUNT          983 a          468 c          254 g         1098 t
ORIGIN      
        1 atttttagtt aaaacctttt gcttgatgta aaattttcat atgttatttt agttaacact
       61 atcattaata taattgagat tactatagaa aaaactatca aaatacttgg tacattatac
      121 ttaaccattt gttggctcat cataatccaa tttggagtca ctgacataaa ttttatagct
      181 ttatctggta taaaacctac actaaagtat ggaattgcca ttatcaatcc acctagtata
      241 aatggaatta cagccgattt aaatatagat gaaagtgtta agcctatttg tgcaaatata
      301 aaagaagtaa aaatagtagt caatataaca attatataca tttgtattac tgtaagattt
      361 aaattactat ttccaaagta ccataagtta tgaatcggtt catttaaatt tttaaaatca
      421 aaaaaactaa aacctctaat tcctactaac aatataaaag aaatattaat aatagataaa
      481 attaatccat tagctactag ttttgataat actactgatt tttttccctt tttagttgta
      541 tttattaaac ctgtcatatt agaatcatat tctttaatgt aatttccaga agttattata
      601 aacattatca aacctattag aggtattgag ccttctatct tgtaaaatat atctgcataa
      661 gtatctaaaa catcaaactt tctaatcaga aaaaatccaa aaaatattaa aaacagccaa
      721 attatccaaa cactctttga ttttaatatt tttatcattt cagctttaac taaatacatc
      781 atttctacta cacctccctt gcatcttcta aatcaaaata aaacatgtat acatcttcga
      841 attttgcttg aactggttgt atattatcct cgcttagaat atcctctgaa acaattctaa
      901 gtactattga attaatatca ctttgaatac ttacaacctt atatttagat tgtatatcat
      961 caacttcaca ttcattttta acttgaacat tatatacttt attttccatg tgacttaata
     1021 tgtctttgtg tgttcctttc attaggagtt tgccatcttt aatcattatt gtttcctttg
     1081 caactgattc tatatcggaa attatatgag tagaaagtat tattatctta tctctagaca
     1141 tttttgctat taaattccta aatctagctc tttcttgtgg gtcaagacct gctgttggct
     1201 catcaaggac tattatctta gggtcgttta gtaaaacttg tgctatccca actctcctct
     1261 tcatacctcc tgagaatttt cctatagact tatttctcac atcataaaga cctactaaat
     1321 ctaataattc tcctatctta ctatttaaat attttcccct taaacctttt aaagctccaa
     1381 tatactgtaa aaattctttt gctgtaaaat ttttataaac tccaaattct tgtggcaaat
     1441 atcctaaaat atttctataa tcagcatcca ttttaaatat atcctcacca ttataaagta
     1501 tctgaccttc atcaggtttt atcaacgttg ttatttgttt cattaatgta gattttcctg
     1561 ctccatttgg tcctagaaga ccataaactc cattttccag agttactgta atcttgtcgt
     1621 ttgctacttt tttaccatat atctttgaaa gattttctat ttctaattta ttcatcttaa
     1681 ctcccctttt atctacttat ataatctgat tttattacta aaaaacataa tgacatagta
     1741 cctatcaata ttgccatcaa aagtcctatt gctatattga caatgtctat attcacttta
     1801 aatatcataa tatcacctag ataatttcct attattttat taaaaccaaa tatcaaatca
     1861 taataattta catttgatac aactgaaagt aattttttag gtaatatatg gattaccgac
     1921 attatcttag ttatagatat aataaatata aaaaaactta ttgaagatat tgaagactta
     1981 cttataaaag acccaaatat ggctatcatg gcccatcctt caaacatcaa aagctctatg
     2041 cctatattta atattacata ctttgttata cttaaattag ttttcaatgc aactacattg
     2101 tctactattc taaaagattc taagtctcca ttaacaggta ctccttgttg catacaagtt
     2161 ataataaata ttcccattaa ataaacagaa taaattacta atggaactaa taaagcaaca
     2221 aatattttag actttaatgc ttttttcttc tcttttgatg atagtatgat atcttttacc
     2281 gaagtaacga cttcatctac gtaaatattg gatataatta tagatataat gattaacatt
     2341 cctacattta taagtgaatt tgtaactcta tagttaaata ctttccaaaa actaatatcc
     2401 ttatattcat tcacacttag attatcaagt tttttctttg acattaattc aattttttta
     2461 gaaaattcac ctgtatctgt ttgatttgaa aactgcttta aaacattaat cttattttga
     2521 aatttctcat tacctatgtc gataccactt ctcgtatctt ctatacgtcc tttttcttta
     2581 tctatataat aactttctgt ttctaaaaca ggcgcaataa agatactaat cattagtatt
     2641 aacactaata tgcctagaga tataaacata ctcttagatt tagccaattt ttttatttcc
     2701 caatttacca atatatttct cctcctaaat cattttaatt acacatatat attaaaactt
     2761 aaatcttata taaatataat actaacctta aataaagctt aaa
//
LOCUS       DAKBNN010000313         2796 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000313 DAKBNN010000000
VERSION     DAKBNN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.319"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(294..929)
                     /locus_tag="OUB02_003738"
     CDS             complement(294..929)
                     /locus_tag="OUB02_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887454.1"
                     /translation="MEHYEKKVSFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNYPTFDTLEKIMDVLGVTPNELLSGEWKYVNQAEKEVCQFLRIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLERYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNAKEDMELLREVLKNNHFDDEDE"
     gene            1396..2487
                     /locus_tag="OUB02_003739"
     CDS             1396..2487
                     /locus_tag="OUB02_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCT8887455.1"
                     /translation="MTTHKNLSVKIDYISIVFETATAEDVIMHILDLPTDIFNVYPAT
                     IKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGD
                     MFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLDEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAVEVLKL
                     QVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSS
                     KLTAHLQRIDRTDLIPYIQYDTKHGKGGI"
     gene            2487..2690
                     /locus_tag="OUB02_003740"
     CDS             2487..2690
                     /locus_tag="OUB02_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCT8887456.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEQVIDKLDAI"
BASE COUNT          871 a          550 c          544 g          831 t
ORIGIN      
        1 aaattcattg aatttcattc atttcaaaga cgtgtatctc tgatttaaaa tacacgcctt
       61 tttgattacc ctacaatctt ccagttacta ttccttatat agcccaaact tgtaccgtga
      121 caatttcatt gatattatcc gatttaccac aaatcaaatt ttatcttttc ttcctcttga
      181 ttaccctcca cagaaaagaa aaagccctgt caagagcctt gccaccattg gtggtgcttt
      241 gcactcttta ctgggctttt tgtttgctgt atcttctaca aagaggaaaa aagttattcg
      301 tcctcatcat caaaatggtt atttttcaat acttcccgta ataattccat atcttctttt
      361 gcatttgggt ttatcatttt tacgacttga taaggtgtcc tatatttatg tccctctatg
      421 ctttccccga acatatccat gctgtataaa tcatcatagc gttctaacag tttttgaaat
      481 ttcaaatgct ggataaattc tttgattgga taaaggtcgg acgctacaat acttttcccg
      541 ttgatgtagt cggtaatata ttcccgtaac ttttccaact catattccag ccattcttcc
      601 tcgctgtcaa agaagttgtc ataatgtcca tgttttagtt ctgcatttaa tcgttcttca
      661 attcttaaaa actggcagac ttccttttcg gcttgattaa catacttcca ttctcccgat
      721 aataattcat tgggcgttac tcccagcacg tccattatct tctctaacgt atcaaaggta
      781 ggataattca cgccacgttc aatcttggaa aggctctgca tattgatacc gattttatcc
      841 gcaagttctt gttgtttcat tcctctgtgt tttcttatgg tttgtatgtt ctctcctaag
      901 aaactaactt tcttttcata atgctccata acttagtata accgctcctt tcgttgcttt
      961 tcttggtatt tataagcata tcatacttaa tatatatcta aaagtcaaca aaaacttctt
     1021 gacaagtaat tctaatggcg ttattatcat atcagttaga gtgattaagc acactagggt
     1081 caatcacacg cttgagtaag catagaaaag atatattttc actttgatag catgaataag
     1141 catggactat cagaccgaaa ataaggtttc tgactggggc tgttccgttt agccacgagc
     1201 cttacaaggc tcgtgagcgt taagaacgga tcagacacag gaagaaaggg aacgccttta
     1261 ggcgttcgta aagggcgtat atgtaacacc gccctgcgta tacatgtcat agtacctaga
     1321 aactgtgata aataaaggaa aaaacacgat ttttaccccg caaaaaaacg gggcatacga
     1381 aaggagcaat gcactataac tacacacaaa aatttatccg ttaagattga ttacatcagc
     1441 attgtatttg aaactgcaac cgctgaagat gtaatcatgc acattttaga tttaccgact
     1501 gacattttca atgtttatcc agcaacgata aaattcaaga cttatcaagc acgctggcag
     1561 attggagata tttatgtatc gggggacgca agaaagacag aggacaaccc acagggacta
     1621 ggctgttatc ttgttatgac tggcagaggt tgtgatgata ttttccgtat tctcgacagt
     1681 aggaattata cctttgggga tatgttccga cgctgtgagc gaagatacgg actggataat
     1741 ttccatttca caagacttga tattgccatt gatgataaga acgaaaagcc attctttacc
     1801 atagagcaga taaagaagaa atgcgaaaaa gaggaattta tctcgaatag tgagggctac
     1861 cactttgacg aaagcaagtt tgatgatttc gacaccgcaa agactgttta tatcggtgct
     1921 ggtaaatcgg gattgtccta ccgcttttat gataaagata aggaagtctg ttcaaaacat
     1981 aataagacac ttgatgaagt cggcagttgg aaacggacag aaatgcaact gcgtgatgat
     2041 aaggctcatg cttttgccat gacattcaaa gacagaccgc tggaacttgg ggaactggct
     2101 ttcgggctat tggcaaacaa cttacgcttt gtcgtgccaa acagaaatga aagtaataag
     2161 agcagatgga aaacgtgtcg gttttgggaa cgctttttag gggctgtgga agtcttgaaa
     2221 ctgcaagtac cgaaactaca taattctctt gaagaaacac agcaatggct cacagagggt
     2281 ggtgtgattt ccgctgtcaa aagtttttac ttcttagaag aacatgacgc attaggtgga
     2341 ctggaaaaag tgggaactat gcttgataag gcaagataca gcaattccct ttccagtaaa
     2401 ctaaccgccc acttacagag gatagaccgc accgacctta tcccctatat ccagtatgac
     2461 acgaaacatg ggaaaggggg tatctgatga ataacaacga tattcctgta tgggaaaaat
     2521 acacccttac cattgaagaa gcgtcaaagt atttccgtat cggagaaaac aagttaagac
     2581 gattggcaga ggaaaacaag gacgctggct ggctcattat gaatggcaac cgcatacaga
     2641 ttaaacgccg acagtttgaa caggttattg acaaattgga cgctatctaa tgcaaatgag
     2701 ccttgtatgt gttatgatga acacaagtca tatcaaggct ctttccaaca aggaaaggag
     2761 cagacaccat gaaagaaaaa agacgggata gtaaag
//
LOCUS       DAKBNN010000314         2790 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000314 DAKBNN010000000
VERSION     DAKBNN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.320"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..544)
                     /locus_tag="OUB02_003741"
     CDS             complement(<1..544)
                     /locus_tag="OUB02_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HCT8887457.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLI"
     gene            complement(706..1923)
                     /locus_tag="OUB02_003742"
     CDS             complement(706..1923)
                     /locus_tag="OUB02_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HCT8887458.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(2114..2527)
                     /locus_tag="OUB02_003743"
     CDS             complement(2114..2527)
                     /locus_tag="OUB02_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887459.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          983 a          416 c          310 g         1081 t
ORIGIN      
        1 caataagttt attccaaata tcaatattgt gtggattttc tttatccatt tttatatttt
       61 gaataagatt gtttaatttt atttgccatt catctttaaa tataaattca tgtccattat
      121 gtattatacc attctggtaa attgtagact caaaagttga actagtcgaa aaaagagtta
      181 ctttttgtga gatagtaggt aattttttaa ctgtttcttc tacaatattt aatattggta
      241 tattaataga ctcttttaat tctttaaagt atatatgtgc tgaattgcat ggcattgcaa
      301 taaaatttac accagttgat tcaagctttt gtaatccatc aattatagtt tctttcataa
      361 gttcatgatt tattggacgg tcaatataaa atggggtagg taatgaatat atcatcatct
      421 ttggaaattc atcatcatat tttgctccat attgagattg acactcatca ataattaaat
      481 caacaaaagg ggctgttgac cttggaccca ttccagccaa aataccaatt aatttttcat
      541 tcataaaaat tatctccttt atatataaat atatttatgt ataaaataaa aatctataaa
      601 agcataaaat tatattttat aatagattat attgtacact tatataatat aaatataaag
      661 tgatttttta gattatatat tttaatatac tttcttatat aagaattatt ttaaatcaag
      721 attatctagt atttttaaaa atatttttac agcagtatcc aagtcatcta cagaaacact
      781 ttcattagga ttatgactta cgcccccagc gcatcttata aataccatac caatatcagt
      841 tatattatcc atttcctgag catcatggcc agcaccacta taaatataaa aaggattaag
      901 atttaaatct ataaaacttt tttctatgat ttttgttatt ttattagaac atggtacagg
      961 aacattttca aaagcaagtt cacttgtata attaagtttt ctattttcac atatatgtga
     1021 tatttcatta aatatctttt ctacagaatt atcaagaatt tcttgtgaac aagaacgtac
     1081 atcgagtgta aattcagctc tttcaggtac cacatttgat gaagatggat agaaattcat
     1141 ctttcctaca gtagcaacca aatcagcagt cgttttagct actttttcga tagctaatac
     1201 acattcacag gcacaacatc cagcatcaag tctcatattc attggaagtg taccagcatg
     1261 tccagactta ccatttactg atatcttaaa acttttaaat cctgtaatac ttgatacaat
     1321 tccagcagga agagattcat tttctaaaac aggaccctgc tcaatatgaa attctaaata
     1381 tgcatctaca tcttttctag gatgcaaatt tgttaagttt tttgtattaa atccaaactt
     1441 tgttacagct tcttcaagtg taattccatt ttcatcaaca gatttaagaa gttcctcttt
     1501 aaaagtacca gcaaaaactt tacttcccat taaagaagtt tcaaatctag ttccttcctc
     1561 ttcagcgaaa gcaattagct taatattgaa aggataagac ctattattgt gttttgcttc
     1621 ttcaagacca actagaggca ctattatacc taacatacca tcaaatatac ctccacactt
     1681 tacactatct tgatgagatg caactactaa tgtgggagca tttggaaagc ttgatttata
     1741 tgtacctata acgtttccaa tgtcatcaat ttcaatatca agatttaaac cttccatcca
     1801 tgaactaatt aaatcaatag attttttatg ttcttgagtc aagataagtc ttgtaagacc
     1861 atttgttgtt gaacttattt ttcctaatgt ttgtagataa tccatacatt tttttcctaa
     1921 catatttacc tcctaaaaat ataattttta tttaagctaa gattagcttt tatacttata
     1981 atttttgaac tttcaaataa ttttgtatgt atagtaaaat actattttag tattaataat
     2041 gatataaaaa tataaagtct gtagacgaag aacagaagtt ttaagttaaa aatatgaaat
     2101 atgattttta ttattatagt atttttctat aataatatct ctttccagtt agaggataat
     2161 tttcaagtga aaatacttta tggtatccaa atttctcata aaatttaggt gcttgaaaac
     2221 tgaatgtatc taaaaaaaca tatttacaac ctcgttcttt agcaattttt tcagccttat
     2281 ataaaagctg acttccagta cctttatatc taagcttctc actaacccat aagtatttta
     2341 tagaaagcca attaccatga gtaacaccta taaaacctgc aacaatttta cctgctttat
     2401 ctcgaatata tattcctaaa tccactgggt ttttatcttc aagtctagca agattatatt
     2461 ttaataatcc ttgaaaaata gtatcttcat cttgtttttc tattttttct gtaatttcta
     2521 attccatatt tgaactttct ccttgtataa tcttttgtat attggtattt tttaataaaa
     2581 tcagtagaaa gaatatagtt taacattatt ctaataggca tttatgctaa tgtcaaacta
     2641 ataatactta ttcttaattt tatattattg atattaaatt tttttaaata aaaatgtagt
     2701 ttaagttata taaaaagaaa tcatcaataa atttagtaaa cctaaattgt tgaatattaa
     2761 aataagttaa tttaatacat aaaaactatt
//
LOCUS       DAKBNN010000315         2786 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000315 DAKBNN010000000
VERSION     DAKBNN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.321"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(294..929)
                     /locus_tag="OUB02_003744"
     CDS             complement(294..929)
                     /locus_tag="OUB02_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887460.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNFPTFDTLEKIMDVLGVMPNELLSGEWKYVNQSEKEVCQFLRIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLKNNHFDDEDE"
     gene            1386..2477
                     /locus_tag="OUB02_003745"
     CDS             1386..2477
                     /locus_tag="OUB02_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCT8887461.1"
                     /translation="MTTHKNLSVKIDYISIVFDTATAEDVIMHILGLPTDIFNVYPAT
                     IKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNCTFGD
                     MFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLDEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAVEVLKV
                     QVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSS
                     KLTAHLQRINRTDLIPYIQYDTKQGKGGI"
     gene            2477..2680
                     /locus_tag="OUB02_003746"
     CDS             2477..2680
                     /locus_tag="OUB02_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCT8887462.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
BASE COUNT          869 a          548 c          548 g          821 t
ORIGIN      
        1 aaattcattg aatttcattc atttcaaaga cgtgtatctc tgatttaaaa tacacgcctt
       61 tttgattacc ctacaatctt ccagttacta ttccttatat agcccaaatt tgtaccgtga
      121 caatttcatt gatattatcc gatttaccac aaatcaaatt ttatcttttc ttcctcttga
      181 ttaccctcca cagaaaagaa aaagccctgt caagagcctt gccaccattg gtggtgcttt
      241 gcactcttta ctgggctttt tgtttgctgt atcttctaca aagagggaaa aagttattcg
      301 tcctcatcat caaaatggtt atttttcaat acttcccgta ataattccat atcttctttt
      361 gaattggggt ttatcatttt tacgacttga taaggtgtcc tatatttatg tccctctatg
      421 ctttccccga acatatccat gctgtataaa tcatcatagc ggtctaacag tttttgaaac
      481 ttcaaatgct ggataaattc tttgattgga taaaggtcgg acgctacaat acttttcccg
      541 ttgatgtagt cggtaatata ttcccgtaac ttttccaact catattccag ccattcttcc
      601 tcgctgtcaa agaagttgtc ataatgtcca tgttttagtt ctgcatttaa tcgttcttca
      661 attcttaaaa actggcagac ttccttttcg gattgattaa catacttcca ttctcccgat
      721 aataattcat tgggcattac acccagcacg tccattatct tttctaatgt atcgaaggta
      781 ggaaaattca cacctcgttc aatcttggaa aggctctgca tattgatacc gattttatcc
      841 gcaagttcct gttgtttcat tcctctgtgt tttcttatgg tctgtatgtt ctctcctaag
      901 aaactgattt tcttttcgta atgctccata acttagtata accgctcctt tcgttgcttt
      961 tcttggtatt tataagcata tcatacttaa tctacatcta aaagtcaaca aaaacttctt
     1021 gacaagtaat tctaatggcg ttattatcgt atcagataga gtgattaagc acgataaggt
     1081 caatcacacg cttgagtaag catagagaag atacattttc actttgatag catgaataag
     1141 catggactat cagaccgaaa atagtggttt ccaaaagggg ctggctggac ggggcacgtc
     1201 ccccagacgt gttcggacag acagttcctt ttggaaaagg aacgcctaaa ggcgttcgca
     1261 gagggcgtat atgtaacacc gccctgcgca tacatgtcat agtacctaga aactgtgata
     1321 aataaaggac aaaacacgat ttttaccccg caaaaaaacg ggaagtacga aaggagtaat
     1381 gcactataac tacacacaaa aatttatccg ttaagattga ttacatcagc attgtatttg
     1441 atactgcaac cgctgaagat gtaatcatgc acattttagg tttaccgact gacattttca
     1501 atgtttatcc agcaacgata aaattcaaga cttatcaagc acgctggcag attggagata
     1561 tttatgtatc gggggacgca agaaagacag aggacaaccc acaggggcta ggctgttatc
     1621 ttgttatgac tggcagaggt tgtgatgata ttttccgtat tctcgatagt aggaattgta
     1681 cctttggaga tatgttccga cgttgtgagc gaagatacgg actggataac ttccatttta
     1741 caagacttga tattgccatt gatgataaga acgaaaagcc attctttacc atagagcaga
     1801 taaagaagaa atgcgaaaaa gaggaattta tttctaatag tgagggctac cactttgacg
     1861 aaagcaagtt tgatgatttc gacaccgcaa agactgttta tatcggtgct ggtaaatcgg
     1921 gattgtccta ccgcttttat gataaagata aggaagtctg ttcaaaacat aataagacac
     1981 ttgatgaagt tggcagttgg aaacggacag aaatgcaact gcgtgatgat aaggctcatg
     2041 cttttgccat gacattcaaa gacagaccgc tggaacttgg ggaactggct ttcgggctat
     2101 tggcaaacaa cctacgcttt gtcgtgccaa acagaaatga aagtaataag agcagatgga
     2161 aaacgtgtcg gttttgggaa cgctttttag gggctgtgga agtcttgaaa gtgcaagtac
     2221 cgaaactaca taattctctt gaagaaacac agcaatggct cacagagggt ggcgtgattt
     2281 ccgctgtcaa aagtttttac ttcttagaag aacatgacgc attaggtgga ctggaaaaag
     2341 tgggaactat gcttgataag gcaagataca gtaattccct ttccagcaaa ctaaccgccc
     2401 acttacagag gataaaccgc accgacctta tcccctatat ccagtatgac acaaaacaag
     2461 ggaaaggggg tatctaatga ataacaacga tattcctgta tgggaaaagt acacccttac
     2521 cattgaagaa gcgtcaaaat atttccgtat cggagaaaac aagttaagac gattggcaga
     2581 ggaaaacaag gacgctggct ggctcattat gaatggcaac cgcatacaga ttaaacgccg
     2641 acaatttgaa aaggtcattg acaaattgga cgcaatctaa tgcaaatgag ccttgtatgt
     2701 gttatgatga acacaagtca tatcaaggct ctttccaaca aggaaaggag cagacaccat
     2761 gaaagaaaaa agacgggata gcaaag
//
LOCUS       DAKBNN010000316         2726 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000316 DAKBNN010000000
VERSION     DAKBNN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.322"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            76..1341
                     /locus_tag="OUB02_003747"
     CDS             76..1341
                     /locus_tag="OUB02_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCT8887463.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            1356..2642
                     /locus_tag="OUB02_003748"
     CDS             1356..2642
                     /locus_tag="OUB02_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCT8887464.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
BASE COUNT         1128 a          219 c          502 g          877 t
ORIGIN      
        1 tgtgagtata tgtaattaaa aaataaataa atttcaattt taaaattgat gtttaaataa
       61 aaataggagg tacatatggt taatactaaa aaaataataa atttaggtaa taatattaat
      121 cttactttga taaaaacaga aaagtttaaa tcgaatctta taagtttata tgttcagaga
      181 ctattggatg aaaaagaaac tacaaaaaat gctctaatac catcaataat agcaagtgga
      241 agcgctaaat atcctagtgc tagagcaata tccaataaat tagatgattt atatggttca
      301 tctatgggag ctgatgctgt aaaacgtgga gaacgtcaag tattgagttt taaagtaatt
      361 aacataagtg aaaagtattt agatgagagt atatttgaag aagtagtaga attttttaat
      421 gaagtaataa ataatacgct tgttgtagat ggtgggttta aagaagaata tttaaatatt
      481 gaaaaagaga atttaagaga aaagatacaa tcaataataa atgataaaaa ggaatatgcg
      541 caagataaat gtattgaggc tatgtgtaaa ggagagagat atagtgtaag tgaatttggt
      601 tatgaagatg aaatagatag tataacttca agagagttat atgaacatta taaaaacata
      661 cttaaaacat cacctataga tatagttgta gaaggtaatt ttgatgaaga taaagttgta
      721 gatataataa gtaaaaatct aaaatttgaa agagaagaaa ttataaatat accaagagca
      781 gattttataa aaaatgtgga tgaggtaaaa gttatagatg aacaaatgga aataactcaa
      841 ggtaagttag ttatgggtta tagagcaaat gtggattatg cagacataga taaatattat
      901 gctttagtag ttgggagcaa tgtcttaggt ggtggtccac actctaaatt atttgttaat
      961 gtaagagaaa aagaaagttt atgttactat atattctctt caatagagaa gtataaaagt
     1021 atcatgttta tatcatctgg tatagaaact aaagattatg ataaagctgt agaattgata
     1081 aaaaaacaag tagaaagtgt aaaagcaggt gatatatctg atgaagaatt agaaaacagt
     1141 aaacttgctt tagttaattc tatgaaatct ataactgata atataggtgg tatgtctgat
     1201 tttgttttct ctcaatctat ggcaaagaca aattctgaag tacaagacat aatatctagt
     1261 atagaaaagg ttacaaaaaa agatatagtt gaagctataa aaaatataga gcttgatact
     1321 gtatacttct tgagaaatta atggaggaga tgtctatgga aaaaatagtt aatgatatat
     1381 tgaaagaaga agtttattat gagaaattgc aaaatggatt agatgtatat tttatgccta
     1441 aaaggggttt tatgaaaaag tatgctattt tagctactaa ttatggttca aatgacttag
     1501 aatttgtacc aattggagaa gataagaaaa ttagagtgaa tgaaggtatt gcacattttc
     1561 tagagcataa gatgtttgag caacctgatg gaggagatgc atttgataaa ttttcaaaat
     1621 taggagtaaa tgcaaatgca tttactaatt ttacaatgac agcctactta ttttctgcta
     1681 ctgaaaattt ttatgagagt ttagaacatc taattgacta tgttcaaaca ccttatttta
     1741 cagatgaaaa tgttgaaaag gaaaaaggta taatagcaca agagataaaa atgtacaatg
     1801 atgaccctga ttggaatgta tactttaatt gcttgaaggc aatgtatgta aattatcctg
     1861 ctagaattga tatagcaggt actgttgata gtatatataa gataactaag gaagagctat
     1921 ataaatgtta taatactttc tataatccag gaaatatggc tttatttgtt gttggtgact
     1981 tagatgtaga aaaagttata gatgtaacta aaaagtcaaa caattacaaa gtagacagac
     2041 tctctaagag tatagaaaga ttttatccag aagaaccaga aggtgtaaaa gaaaaagagg
     2101 ttatagaaaa attccctata tctatgccta tgttcaacat aggttttaaa gatagtaatg
     2161 ttggtttaaa agggaaagaa cttttacgta aagagattgt aactgatata cttgtaggga
     2221 tgttatttaa aaaaggaagt aagctttatg aagatttgta tatgcaaggt ttaataaatg
     2281 aaaattttgg agctggattt tcgtctcaag tggattatgc ttttagcata atagctggag
     2341 attctaaaga gcctaaaaaa gtaaaagaaa taattcttga ttatatagaa aaatcaaaaa
     2401 aagaaggatt atcaaaagaa gagtttgaga gaactaaaaa gaaaaaaata gggtcattta
     2461 ttaagtgctt tgattctata aactttatag gtaatagttt tatatcttat gtttttaagg
     2521 atataaatct attggagtat ttagatgtta ttaaagatat tacttttgag gaagttgaag
     2581 aaagattaaa agagcatttt aaagaagagt attgtgttat ttctattgta gagcctaaat
     2641 agaaacttaa tcataataag aataactaat taagtattag tttatgtttt taaatgttta
     2701 ttttggtaaa acaaatttat aaaata
//
LOCUS       DAKBNN010000317         2725 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000317 DAKBNN010000000
VERSION     DAKBNN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.323"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            299..955
                     /gene="nth"
                     /locus_tag="OUB02_003749"
     CDS             299..955
                     /gene="nth"
                     /locus_tag="OUB02_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCT8887465.1"
                     /translation="MKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILS
                     AQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLC
                     ELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPN
                     PQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK
                     "
     gene            1149..1619
                     /gene="trmL"
                     /locus_tag="OUB02_003750"
     CDS             1149..1619
                     /gene="trmL"
                     /locus_tag="OUB02_003750"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCT8887466.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1655..2503
                     /locus_tag="OUB02_003751"
     CDS             1655..2503
                     /locus_tag="OUB02_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCT8887467.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         1128 a          262 c          521 g          814 t
ORIGIN      
        1 aatggataag aaagatataa ttgagaagga agagataaat aaaaaaggaa tgaataagaa
       61 agatataatt gagaaggaag agataaatga aaaaggaata gataaaatga ttggtaaaaa
      121 agatgagata attgatgaga aagatataga gaattttatg gatattgaag aagataaaaa
      181 aagtatcaaa gtaagtaaaa aaagtactaa aaaaggtgaa acggagaaaa aaacaaagaa
      241 gagtagttct aataaggcta cacaaaaaac aagtaaagaa actaaaagta agtctaaaat
      301 taaaaatgag tcagcaaatg aaaaagacgt aaataagatt ttagacgagt tagaaaaatt
      361 gtatccagat gctaaatgtg agttaaatta tgggacagcc tttgaacttt taatagcaac
      421 tattttatca gcacaatgta ctgatgtaag agtgaataaa gttacttcag agttgtttaa
      481 aaagtacaac acagctagag actttgctaa tttgagcatt gaagaaatta gtaaagagat
      541 aaagagttgt ggtctttaca aaagcaagtc tcaaaaaata aaggatacat ctgaacagct
      601 atgtgaatta tatgatggtg aagtacctga tagcttagaa aagcttataa aattacctgg
      661 tgttggaaga aaaacagcag gggttgtact tagtaacgct tttaatcatc cagcaatagc
      721 tgtagatact catgtattta gagttagcaa tagaatagga atagtggacg agccaaatcc
      781 tcaaaaaaca gaatttgcac ttatggaagc tataccaaaa gaaagatggt cacactcaca
      841 tcatgtatta atttttcatg gaagaagaat gtgtaaagct agaaatccag aatgtgcaag
      901 ttgcccaata aaagaagatt gtaattatta taaagaactt aatgaaacga aataactttt
      961 agaaagtaag ttgtacaaga gggattttct gatataataa tacttgtgaa aatgttagat
     1021 aaaaaactat tatatacatg aaataaaaat tattattaac attattttat ataatagtat
     1081 aatattattg ttattctgtt tataaaataa aatttgataa ataaaagcga tacttaggga
     1141 ggattaaaat gtcattaaat atagtattag tagaaccaga aatacctcaa aatacaggaa
     1201 atataataag aacctgtgca gcaacaggtt caactttaca tttagtaaaa ccactgggtt
     1261 tttcattgga tgataaacac ctaaaaagat gtgggcttga ctattgggat atagctgata
     1321 tacaatatta cgatagcttt gaagaattac aagaaaagta tccaaatagt aaatactttt
     1381 tttctacaac aaaggctaaa cagtcacatt cagatgtgaa atatgaagaa aattgtttta
     1441 tagtatttgg gaaagaaaca aagggattac cagaaccttt gttgaaagca aatatggata
     1501 cttgtattag agtgcctatg ttggatgttg aaaaggctag gtcattaaat ttatcaaatt
     1561 cagttgctat tgtagtgtac gaggctttaa gacaaatagg atacccaaat atgaggtagt
     1621 ttcaacaagc ctaaatgaga aaggagcata cttaatgaaa aatctaaaga taatctgtga
     1681 cagtttatcg gacgtgccaa aagatttgtt agataaatat gatatagata tagtttcatt
     1741 aacagttata ctggaggaaa gagaatacaa ggatagaatt gatatatctg gagatgaatt
     1801 ttataaaagg cttagagaag aaaatgtata tccaaaaact tctcaagcta cttatgccca
     1861 atttaaggaa atttttgaga aatatacaaa tgaggataga gagatacttt atatagcagg
     1921 gtctgccaca gctacaggta cgtatcaaag tgctgtaatg gcaaagaatg atgtagatgg
     1981 taaaatatat acatatgact ctaacttgct ttgtatggga actggtttac ttgtaataaa
     2041 agcaggagaa cttgcaagtg aaggaaagtc tgttgctgaa atattgcctg ttttggatga
     2101 attaaaagag aaggcttttg ttatattttc agtagataca ttggaatatt tacaaaaggg
     2161 aggtagaata tcatctacta aggcagcaat aggaagtata ttaaatataa aaccgattct
     2221 tgaaataaga gatggattag tttcgcaaat atctcaagta agaggaaaga agaatgtaat
     2281 ttctaagatg atggaattga taaaatcaaa ttgtggtgat gatttatcag accaaattgt
     2341 gtatatagga tatagtgatg attttaagga aaaggaacaa ttatctaagg ttatgcaaga
     2401 tgaattaaga cctaaagaaa ttagattttt ccaaattggt gtgtgtatag gtgcacatgc
     2461 tggtcctggt gttactggta tcatatgctt aaaaaataaa taatatattt ttgggtgaca
     2521 ttatttattt ttttattaaa ttaggataga atattaatta ttgagatgtt aaattggatt
     2581 tattttgtat gaaattaagt gttgatatat tagatttagt ttgtagggaa gtgtgtaaat
     2641 agataagaat ctattgcaca cttttttata gtaagattta ttaaaaaaat ttcaaaatgt
     2701 acttgtaaat tgtatataaa aaaat
//
LOCUS       DAKBNN010000318         2677 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000318 DAKBNN010000000
VERSION     DAKBNN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.324"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(269..1399)
                     /locus_tag="OUB02_003752"
     CDS             complement(269..1399)
                     /locus_tag="OUB02_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCT8887468.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(1639..2145)
                     /locus_tag="OUB02_003753"
     CDS             complement(1639..2145)
                     /locus_tag="OUB02_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCT8887469.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(2223..2564)
                     /locus_tag="OUB02_003754"
     CDS             complement(2223..2564)
                     /locus_tag="OUB02_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCT8887470.1"
                     /translation="MATFGERFKFLRTEMNLTQDELVEKFNKVYLTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVATHKANPNEILPEEAQEQLND
                     YIEFLINKYKK"
BASE COUNT          789 a          355 c          360 g         1173 t
ORIGIN      
        1 tcaaataaat caatgactat ataattgaga acttattaaa ttttaattat acaatttgga
       61 atagtattac aacttaagta aatattaagt tttatttgtt agatttatta ttaatacctt
      121 atattagtta tggttgttat atactctaaa gccttgaaat tacaagtata tagatgatta
      181 tataaatttg ataattttag cttttaaaat agataaatta gattttgcca cccatttgcc
      241 accgtattat atttttgggt ggcaaactct aatttgtaat tttttcaaat atatctacag
      301 tttcattttt cattttatca gttacatgtg aataggtatc cattgtagtt gatagttggc
      361 tatgacccaa acggttttgt atgtctttaa tgttagcacc attttctaat aatagggtag
      421 catgtgcatg tcttaaagaa tgaaaatgga agtcattgtt taaagctact cgaatttgtc
      481 ttactatagt gtctaaagtg tgagtattca cttgttgacc attttctttg gtacataccc
      541 agtcactatc aaagtaaaat tctccatatt ttaatttcat tttcttttga tataatttat
      601 gttcttttaa tgcctttatt aaagtgtcac ctgtaaatat agttctgcaa gagctttctg
      661 tttttggttg ccctaattca aacattccat ttggcttttt aatcagagta tgtcttactg
      721 tgatagtttt attattaagg tctatattat cccattttag tgcaataatt tcacctcttc
      781 gcataccagt atgaaatcca attagtaaaa ctatacgttg aaatgaatct tgaggaaata
      841 tatttagtat ttgattaaat tcttctaatg taatagtttt aactttatta gtttctgttt
      901 tagatttagt ttttggtatg cttacatatt gcatagggtt ttctcgtatg tgtttgtaag
      961 gatggactgc tgattttaat gacctatgta atatggcttt taatacttgt aatgtatttt
     1021 gagagtaatc ctctttgtac tttttattta tgaagttttg tagtattgca ggagttaaag
     1081 cttttacttt gtaagctcct agttttggct ttatatgttt ttctatgttt attcggtagc
     1141 tttcttgagt gttgtattta cagttaagta agacatattc tttgtaccaa aaatctaagt
     1201 agtctgataa actgatgttg ctttcttcaa atactatgcc agagttttca tattcattta
     1261 gtgcttctct taaggctttt tcggcttctt ttttagtatt gcctccaact ctttctactt
     1321 tttttctttt tccttctact atgcctaggt caaagtagta ataccatttg ttacttcttt
     1381 ttcttactcc acctttcata atagtatccc tcccttttag aatgtatgtt tgtttggtgt
     1441 ttatataaaa gagcagatta actgctcttt atatactttt gttatgtaca atattatctt
     1501 aattttgttt atctggtttt tacttaatta ttttcttaat attcttacaa ttttttagct
     1561 tttatgtgta gtttattata ataatattga aatatatatt tattaagtaa aaaatctata
     1621 aaagtagaat aaatgagttt aaaaaatatc ctcatatatg tagaaatttg gcaaattagt
     1681 taatactaaa aatctattat ttcctaagtc caacatgctt ttcttttttg atagaaattc
     1741 taatcttttt agtaagaagt tgatgctaac ttgtaattct tcagctattt cgtatacact
     1801 tgtagcgtgt gaattaataa catgtattat ttcttcttct gttataagaa attcacatgc
     1861 ccattttaat gctttgtttt cagttttgtc tatcaagatt ttatttttgt aactgttttt
     1921 tgaagataca tagttcccaa cactggtaaa atgatgtcct aattcttctg ctaagatttc
     1981 tattagttta gcattgtttt gttttaatga attaagtaat gatataatct ttagtccttg
     2041 tctgtttata tacaatcctt ttatgtcatc tgctattttg tcagtgtagt aaatttctat
     2101 ctcttcatta tttgctaagt ctaaaagtgc gtctagtttg ttcattgaaa tccccctata
     2161 aaaagaatgt atgtgttact tttttatata tataaagagc aggagtgaac tgctctaaat
     2221 attcattttt tatacttatt tattaaaaat tcaatataat cattaagttg ttcttgagct
     2281 tcttctggta aaatttcgtt aggattagct ttgtgtgttg ctacagtatc tatgtgattt
     2341 ctaacaagag ttcttcctaa aaggtaatca attgatacat caaaaaaatc tgcccaattt
     2401 tccaaaatat ttatctctgg ttttcttttg ttattttcat attgtgatat cgtcgattta
     2461 ttaaaactcg taaggtaaac tttattaaat ttttcaacaa gttcatcttg agttagattc
     2521 atttctgttc ttaaaaattt aaacctttca ccaaaagttg ccataaataa aacccccctt
     2581 tatgctaatt atagcataag tttatgaata tgtgaactta ttttgcgaaa gttaacataa
     2641 aaatgaattt atatattgac aggttttgtt tgtgtta
//
LOCUS       DAKBNN010000319         2617 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000319 DAKBNN010000000
VERSION     DAKBNN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.325"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            19..1113
                     /locus_tag="OUB02_003755"
     CDS             19..1113
                     /locus_tag="OUB02_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCT8887471.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKREKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            1115..2065
                     /locus_tag="OUB02_003756"
     CDS             1115..2065
                     /locus_tag="OUB02_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCT8887472.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRAKHG"
     gene            2058..>2617
                     /locus_tag="OUB02_003757"
     CDS             2058..>2617
                     /locus_tag="OUB02_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887473.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVID"
BASE COUNT         1078 a          206 c          486 g          847 t
ORIGIN      
        1 taaagtaagg tgttgtctat gaaaaatata gttggaaaac cgcaaacgat aaaaaaagta
       61 aatgaagatt taataaaaaa cattataaaa aatgaaggtc ctataacaaa accagaaatt
      121 tcaagcatta ctgaattgag tttagctaca gttaataaga ttgtagagca attagctttg
      181 aaaaatgaag ttaaagtaag tggactaagt gaatctactg gaggaagaag agctcaatta
      241 tttgaaataa atgcaaactt agaacacata atagctttat attattatag aaattgttgt
      301 ataggggtag ttgccaattt attaggtgaa ataatttatc aagaagaatt taatataaga
      361 atggatacgt atgaaaacgt taaccaagat actttttttg ttatagatac tctaataaaa
      421 agggaaaaag gaaacaatat aagggctatt ggaattggtg tcccaggagt tgtaaaaaaa
      481 ggtgtagtta aaaatatacc aactataaaa agttgggaag gtataaattt agaaaattta
      541 ataaagagta aatacaatac taaagtgttt atagaaaatg atacaaatat tactactatt
      601 gggatttatc aaaaacagta taaagacaaa tataaaaata tggctcttat gtatttagaa
      661 gatggtattg gttcaggtat agttataaat ggtgaattgt atttaggttc tacaaatttt
      721 gctggagaat taagttatct aaatatagaa tttggtaagt ataactctaa caacttaaat
      781 cttgaagatt atattatgag tttaagaaaa agatatttag aaagtaaaga taataaattt
      841 aagaaagaaa ttttatctat aatatcaaac tttataagga ctcttgtatg tgtcctaaat
      901 ccacaagcta tgattataca gtgtagtata ttaaccaaga gtgacatgga gtttatagaa
      961 aaagatgtaa gcaataatgt tggagaagaa aattttccta aattaataaa agtagagtca
     1021 ttgagagaag gaagtataaa tggagtaatt agtatgtgtt taaaagaaac tgactatcaa
     1081 tattccttat ctaataaaaa aggaggatat taatgtgaaa tatttggttt caatagatgg
     1141 agggggaaca aaaactaaat tttgtgtaag tgacttagat ggaaacatac ttaaagaaca
     1201 taccactggt tcaactaatt ataagtcagt tggaattaaa aaaacatatg aaaatattaa
     1261 taatggattt aaaaaaatat taaaagattt atatatcgat tatgacgata ttgagtacac
     1321 tgtatttggt atatctggtt gtgattctcc taatgattat aagattataa tggatgaaat
     1381 attaaaaatt gggattaaca aagaaaaaat atatctggca aatgatgcag ttttagcttt
     1441 ttatgcacaa gctgattccc ctggtttagt aattgtggct gggacaggtt caattatact
     1501 gggaattaaa gaagatggag aaatatatag agttggtgga tgggggtata attttagtga
     1561 tttgggttct ggatatgata tagggagaaa attattaaag aaagtattgc tttattgtga
     1621 tgaatgtcat gagtatagtg atttatttag ttgtgtcttg gatttctttg gagcaaatag
     1681 ttttgaacaa cttgcatata tgattactga tataaatgat aatgttgaaa tagcaaactt
     1741 agcttcatta gtgattgatt gtgccgaaca aggtgataaa ttggctattg aaatattaag
     1801 agaatcatca actgaactat ctaaattagc tcaagtaata ttgagaaaaa tatctaatcc
     1861 aatgaaaatg gataaaagcg aaataaatat agttttgtct ggtggtacat taaataaaac
     1921 tgtatatgct caaatgttaa tgaataattt aaaaacagaa aatacagata aaaatttaaa
     1981 atttatatct caagaaaatc agccagtata tggggggata agacttgcta tggcattagc
     2041 taataggagg gccaagcatg gatagaaaaa aggtaatagt acaaattttt actggaggtt
     2101 ttaaggctaa agaaataaag tttaatgata taaaatgtaa actactagaa ttattggact
     2161 gtattgatat agaaaaggtt atcatgggat ggagtgtaga tgaagaatta tatgtagaaa
     2221 ctaaaaaact attaaataaa tataatgttg aactatatct ttggttgcca tgtttttctg
     2281 aaataggatt acttgaaaaa agtaatttac taattgatta tatggggaag gaagtcaagg
     2341 gctatgcttt acaagaggat gaaaattttg agttttattg cccaaataat aaggaaaata
     2401 taaaaaatat aaagaaaata tatagtactt attttagtga tattaaattt gatggaattt
     2461 ttttagataa aataaggtat ggttcttttt cgaataaact aaatggtgtt tttaattgtt
     2521 tttgtaaaga ttgtttaaat atatatgaaa aaaataatat tgataaagag tcgttaatga
     2581 gagaaatgaa taatgtgtct aagggagtta tagataa
//
LOCUS       DAKBNN010000320         2586 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000320 DAKBNN010000000
VERSION     DAKBNN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.326"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            141..1739
                     /locus_tag="OUB02_003758"
     CDS             141..1739
                     /locus_tag="OUB02_003758"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HCT8887474.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1974..>2586)
                     /locus_tag="OUB02_003759"
     CDS             complement(1974..>2586)
                     /locus_tag="OUB02_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HCT8887475.1"
                     /translation="GPTGNTGVTGEIGLTGATGPTGNTGATGSIGPTGVTGPTGATGV
                     TGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANGTFT
                     VQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYFAG
                     FLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          910 a          375 c          400 g          901 t
ORIGIN      
        1 tgtagtattt atgtaagaaa taaattttat ataatatttt ttcactaaat ttttttcttt
       61 ttacaacaaa tactcactac cttttgatat aatattttta tcaattatta ttatattcaa
      121 gttttgaaag gagtattaaa atgagtttat tagttttcgg acataaaaat ccagatacag
      181 attcaatatg ttctgcaatt tcattagcac atttaaagaa caaactagga ataaaagcaa
      241 cagcttatgc tttaggagat atcagaaaag aagctaaata tgccttggat tactttaagg
      301 tagatgcacc tgaaatttta gataatgtta gaatacaagt tagagattta aattatgata
      361 aagtagtacc tttaacacca acttcatcta tattagaggc ctataacctt atggatgaaa
      421 aaaatgttaa aacactacct gtaacttatg atgatggaac ttttgctggt atcattacta
      481 tgaaagaaat agctaaaaat ctacttcatc aacactttac aactatacac acatcactat
      541 caaacatttg taaaaacctt aatggtacta ttcttgtgga ctgtggtgaa gatataaaag
      601 gtagagtagt cactttatca tttggaatgg agactctaat agagacttta aaagaaggag
      661 acatagctat tgttggagat agatatgata gtattgaata tgctattgat acaaaagtta
      721 aacttttaat acttactaat catacagaga tttcaaaaga tttgctagct cttgctaaga
      781 taaatggagt gtctgttgtt tctgttgaaa gtgacaatta taaaacttct aatgtaataa
      841 atcaatgtag tttcttagac tctatagaag ctacagaaaa tttaattaca ttcaaagaag
      901 atgattatat ggatgaaata aaagagatta tgctagaaac aaacttcaga tcttatccaa
      961 tattagatga tgataataag tttttaggtt tagtttcaaa aggtcatcta ttaaacccat
     1021 ctaaaaagaa tgttgtttta gttgaccata acgaatatgc tcaaagtgct gatggaattg
     1081 aacaagcaaa catagttgaa ataattgacc atcataaact tggtggtatt tctactgatg
     1141 ttcctatgtc atttagagtt atgcctgttg gatgcaatag tactataatt tatcaaatgt
     1201 acaaagaaaa taatgttgag ataccttatg aaatagccgg gcttctttta tctgctatat
     1261 tatcagatac attattattt aagtctccaa caacaactga tatagataag aaagcttgtg
     1321 aagaattaag taaaatagca aaagtagaca tggaaaaata cgctatggat atgtttaaat
     1381 gcggtacttc tttagatgaa tatactatag aagaaatagt aaatatggac tttaaagaat
     1441 ttaatatgag tggacataga gttggtattg gacaagtatt taccttagac atagactcta
     1501 tatttgctaa aaaagatgag tttttatctt atataaattc gactgattat gataaattaa
     1561 ttctttcagt tacagatata atcaaagaag gttcttattt aatctataaa gcagaagata
     1621 gcttaattgc taacgcattt ggtgttgaag gtgttcaagg cacatttgca ccaggtgttg
     1681 tatcaagaaa aaaacaatta gtccctaatt taactactgc aatcaaaaat tttaaataat
     1741 ataaaatttt taaataacga aaactgtctc aaatctatat ttgagacagt tttttattat
     1801 atatatattg ataataaagt taaataaaaa tccataaatt taaaattttt atgaatatat
     1861 tttattatta ttgttatttt attcattcta aaataggtaa aactcaatat ataaaaatac
     1921 ttttgtcatt taaataaagc ctaaatagtt tttaagatta ttttagacac gttttaagtc
     1981 ataacagtat cagctattct aaatatcgtc aaagtagccg cagttcctgc tgattgccca
     2041 tttcgtatcc ctactgttgt agatgaaaaa ttatttattg taaaagtagt cccaccaaat
     2101 aaaaaaccag caaaatatcc agaaagtact cctccgcctc ctggctcagt agtaagccct
     2161 gccaaatctt ttcctcctaa aattgggaat aggatagcga aagaatattg atttatactt
     2221 gatgaacctg gttgtaatgc tactgaaact gatatataat acacaccatt ttcttgtact
     2281 gtaaacgtac catttgcatc tactgttata cttgaactat ttcctattat tgtttgccaa
     2341 cctgttatag catctccatt atttagtgtt tgtggagatg gtgcatttac gaggaagtta
     2401 gcaactggct gagaggaatt gcctgttgct cctgttggac ctgttgggcc tgttactcct
     2461 gtcgctcctg ttggtcctgt tactcccgtt ggccctatac ttcctgtcgc tcctgtattt
     2521 cctgttgggc ctgttgctcc cgttagtcct atttctcctg tcactcctgt atttcctgtc
     2581 ggaccc
//
LOCUS       DAKBNN010000321         2540 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000321 DAKBNN010000000
VERSION     DAKBNN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.327"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            595..1644
                     /gene="cfr(B)"
                     /locus_tag="OUB02_003760"
     CDS             595..1644
                     /gene="cfr(B)"
                     /locus_tag="OUB02_003760"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(B)"
                     /protein_id="HCT8887476.1"
                     /translation="MQQKNKYIRIQEFLKQNKFPNYRMKQITNAIFPGRINNFNEITV
                     LPKSLRDMLIEEFGESILNIVPLKAQQSTQVSKVLFGISGDEKIETVNMKYKAGWESF
                     CISSQCGCNFGCKFCATGDIGLKRNLTSDEITDQILYFHLQGHSIDSISFMGMGEALA
                     NVQVFDALNVLTDPALFALSPRRLSISTIGIIPNIKKLTQNYPQVNLTFSLHSPFNEQ
                     RSELMPINERYPLSDVMDTLDEHIRVTSKKVYIAYIMLHGVNDSIEHAKEVVNLLRGR
                     YRSGNLYHVNIIRYNPTVSSRMRFEEANEKCLVNFYKELKSAGIKVTIRSQFGIDIDA
                     ACGQLYGNYQKTNSQ"
     gene            2345..2540
                     /locus_tag="OUB02_003761"
                     /pseudo
     CDS             2345..2540
                     /locus_tag="OUB02_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
BASE COUNT          923 a          335 c          464 g          818 t
ORIGIN      
        1 tataattcgt ccatacgaat tacctcctgc aatatagtca gagaggaaac ccctctacct
       61 aatagccaca ggagataatg attgttgagg attttgaaaa aattaatggg tatttggatt
      121 attgtgcgga atttgtgtat gttgaaatgg atttaaaatt gattcagcaa tttttctact
      181 ttaacaaacg ccctttaatt gaataactta ctttaaagaa aactggatat ttatgttgaa
      241 ataagaacaa aattgtgaat ggtttaactt aaatgttaac ggtaaacggt gcaggttagt
      301 ttaactacat ttatatccaa aattgtttgt ttttcaaatt acatgtattt ttttgagttt
      361 acatagggaa agatttatgg tatactattt aacacttata ttgacaagaa agaggtgaaa
      421 gcaatgttaa ctgtttttag aattttgttt ggtaaaaatg caaaagttgg taatcgttcg
      481 gcatacaaag ggagtagtct gtcctttttt aagccatata accatacaaa aacagttatc
      541 ggagcttttc ctctacggca aacaaaccag agttatatgg aaggatgaga aaacatgcaa
      601 caaaaaaata agtatataag aattcaagag ttcttgaagc agaataaatt tcctaattat
      661 agaatgaaac aaattacaaa tgctatattc ccagggagaa taaataattt caacgaaata
      721 acggttcttc ctaaatcact aagagatatg ttaattgagg agtttggaga atcgatttta
      781 aatattgttc ctttaaaagc acaacaatct acacaagttt caaaagtctt atttggaatt
      841 tcaggagacg aaaaaataga aacggtaaat atgaaatata aagctggttg ggagtcattt
      901 tgtatatcat cgcagtgcgg ttgtaatttt ggttgtaaat tttgtgcaac tggagatata
      961 ggtttaaaac gtaacttaac ttcagatgaa attactgacc agattttgta ctttcactta
     1021 caagggcatt caattgacag tatttctttt atgggaatgg gagaagcatt agcgaatgta
     1081 caagtttttg atgctttaaa tgtacttaca gatcctgcgt tgtttgcttt aagtccgcgt
     1141 aggttatcta tatccactat aggaattatt ccaaacatta aaaaattgac tcaaaactat
     1201 ccgcaggtca acctgacatt ttcattacat tctcctttta atgaacagcg aagtgagtta
     1261 atgccaatta atgaacgcta cccattatca gatgtgatgg atacattaga tgagcatata
     1321 cgagtaacct caaaaaaagt ttatattgct tatattatgt tgcacggagt taatgattct
     1381 attgaacatg cgaaagaagt cgtaaacctt ttaagaggta gatataggag tgggaacttg
     1441 tatcatgtga acatcattag atataacccg actgttagtt cacggatgcg gtttgaagaa
     1501 gcaaatgaga aatgtcttgt caacttttat aaagaattaa agtcagcagg aattaaagtt
     1561 accattagaa gtcaatttgg cattgatata gacgctgctt gcggtcaatt gtatggaaac
     1621 tatcaaaaaa ccaatagcca gtaacttgag gtgagagtga tgtttagaac gtgggaagta
     1681 ccctaataat ttatttagta acgataaata aacttattag gatatttatg ataaaaggaa
     1741 tcttagaaag aaaaataggc atcactcaag tggagctaaa aagtcgtttg taaaaattga
     1801 aactgcaatt aaattattga atgtgaaaaa tggtgatgat tagtgttaaa caaaaaaata
     1861 atttcagtca aagtatcagt agagatcatg tagtcattac ggatttttgg acgttgactg
     1921 acaataatga aattcaacta ccgtccaaca tggaaattat atgcaataat tatttgaaga
     1981 gtttgaaatt gatgtatcag aagattactc taaacagtag ttggaaaaat ttgtcatgcg
     2041 agaattgcgg atgcagtgaa ttattgataa tcaggtggaa gacagtagtg tatccaatga
     2101 agagatgaac acgaatacca aaaatcactt aaaataaggt ggatatgcaa aaggaataca
     2161 atcgtgttat ccttgatatg aaaagcaaga aaagaaatgg gaaacatttg ttctcaattt
     2221 aaaaaggcaa cgtcaattga aatatttata atttcgctta tctaaaaggc cttagagcat
     2281 attttaatga acaaaattga aaggactgaa cctataataa tgattcacta atccataaac
     2341 tattttgtat ttatacttta cagccatttt aagaagtttt ggcaatacta tgtttcaagc
     2401 agttgcaatg cttgtgtcta ctttattgaa tgctatttta gatcctattt tcattaacat
     2461 aattggattt cacggagcgg caatcgcaac cttaatatca cagattattt gtttggcgtt
     2521 tatgtgcatt tatatttatc
//
LOCUS       DAKBNN010000322         2533 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000322 DAKBNN010000000
VERSION     DAKBNN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.328"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(48..485)
                     /locus_tag="OUB02_003762"
     CDS             complement(48..485)
                     /locus_tag="OUB02_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HCT8887477.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVYDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(742..891)
                     /locus_tag="OUB02_003763"
     CDS             complement(742..891)
                     /locus_tag="OUB02_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HCT8887478.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(904..1134)
                     /locus_tag="OUB02_003764"
     CDS             complement(904..1134)
                     /locus_tag="OUB02_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCT8887479.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1993..2214)
                     /locus_tag="OUB02_003765"
     CDS             complement(1993..2214)
                     /locus_tag="OUB02_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCT8887480.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
BASE COUNT          833 a          294 c          317 g         1089 t
ORIGIN      
        1 caataaattt gcaattagtg atattaataa attactttag atttttatta aatgtttagc
       61 ttttctttaa tattttttat ataacgacga tttataataa ctttttcgtt gttttttaaa
      121 gtagcttgca tgcgattatg tgatattact tttatacttt ttatgtagtc catatttaaa
      181 atcatggatt tagatattcg tgagaaggat gtattagcta ataattttga taatttatat
      241 atttttaaat caatttcaaa tacatcatct tcataataag caaatgtttt atttccaact
      301 gtctcaaagt ataaaatatc atagacattg agtttataaa tctctcctga ttttttacct
      361 tttatatcaa gtaacggtgt actcaataat gaaattattt tttcaatgtt attatctaga
      421 gcagaacatt ttatggttat ttcaatatca tcacaattaa taccttgttc gacatgtatt
      481 ttcattaatc atcacctcat tttattactg tcattagaat agcattatat cttattgagt
      541 ccaactagat gcgttctaaa tttcaaatta agcatagtga aattcaaaaa taaaacagtg
      601 aaattcaaat ttgaggtggt aaaattcaaa aaaatactat ggatatttgg ataaattatc
      661 caaatatcct agcattttac atagaatatg cctaattatt ttacaaatag acctatttta
      721 tgacttacat atattgtcaa attacttctt aaatatcaaa atatttttgg ataatgtatc
      781 aatactgttt gataaagtat ttaatttacc ctcaattcta accaataagt acatggatag
      841 agctatagga aaaccaacat tggttattaa cagttgtaaa ctatcatcca taaaattagc
      901 cccctattct ataactaagt cataaggagt ggtatttgtt tccacaacct tggcttcaac
      961 cacagaagct aggtcaaatc ctccaaccga aaaaatattt ttagatacta tcaaatccat
     1021 gacagatttt atattttctt cagttaaatc atcctttgga ttttctatag aaaaagaaac
     1081 ttgtttatcc tttacatttt taaagctcat gactaatttt ttcttaattt ccatataatt
     1141 acctccgata taataattta tattttaatt gtttgcttgt taatattggt attttaattg
     1201 tcaatattag tgttttgctt gttagtatta atatttcaat tgtcaatatt aatgttttgc
     1261 ttgttagtat taatatttca attgtcaata ttagtgtttt gtttgttagt atcagtgttt
     1321 caattgtcaa tattagtgtt ttgcttgtta gtattgatat ttcaattgtc aatattagtg
     1381 ttttgcttgt tagtattgat atttcaattg tcaatattaa tgttttgctt gttagtatta
     1441 atatttcaat tgtcaatatt agtgttttgc ttgttagtat tagtatttca attgtcaata
     1501 ttagtgtttt gcttgttagt attagtattt caattgtcaa tattaatgtt ttgcttgtta
     1561 gtattagtat ttcaattgtc aatattaatg ttttgcttgt tagtattagt atttcaattg
     1621 tcaatattag tgttttgctt gttagtatta gtatttcaat tgtcaatatt agtgttttgc
     1681 ttgttagtat cagtgtttca attgtcaata ttagtatttt aattgttgac tctagttatt
     1741 gctgatatag aaaatattta atttatttca attaattaga ttaaagtagg gtagcatcta
     1801 aaatagctaa catgtttaag ttgaaatacg tttaatatat taatttttca aagcgttaat
     1861 atattaaagt tataaccaaa attgaagtta taatctgcca aatctatagc gtattaagta
     1921 cgttatataa cttaatcttt tatataaaga gttaattttt taatttgtaa agcagagtac
     1981 gtttaaaaga tactatttag atagtgatgt actatctaat tttaaaatat ctacaatagg
     2041 gttctcttga agacttgcta ttgcttctgc aacagcataa acatcttcat ttaatgcctc
     2101 tgcttttaca tttgcaaatg atttactttt aacaactgcc ttacccttat catcaaaacc
     2161 agcatccaat tttagtttta tactagataa attttttgtt gaaacaactt ccataaaatt
     2221 acctcctaaa aatttaattt caaagatttg tcgactttct ttgttagcta taatactatc
     2281 atctaagata atatcggagt taaacagtaa tttctaaaga ttttttgtgt aaatttttat
     2341 aggaaaaatt ttactttttt gctgatgaaa aagtttacaa gttatttgtg aagatatgtt
     2401 cttagtgttt tgaaatagta tttgtatata ttcctgctta gttgagatag ttttgtatgg
     2461 attttcatat gtgtagagtt aaatgttagg tactaaagtt tatgttaaat taaatgttaa
     2521 agcttatgtt aaa
//
LOCUS       DAKBNN010000323         2528 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000323 DAKBNN010000000
VERSION     DAKBNN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.329"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(798..1835)
                     /locus_tag="OUB02_003766"
     CDS             complement(798..1835)
                     /locus_tag="OUB02_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8887481.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNSTKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVFEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(1932..>2528)
                     /locus_tag="OUB02_003767"
     CDS             complement(1932..>2528)
                     /locus_tag="OUB02_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCT8887482.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT          832 a          376 c          324 g          996 t
ORIGIN      
        1 cctttgaaaa tatttttata attttaatta tttgctaatt ttgtgtataa aaaaaccaca
       61 tagtgtacaa aactatgtgg taaaaacacg aataagccac atagtttaat ctatgtggct
      121 cttatatagt taaatgaaaa ctttataaac aatatagcca acatagatac aatcccaaaa
      181 ttttagtagt cttaaggggt ctgtatctat gttaaaacgt aggtacaaac cctaaaaata
      241 gtggctataa taataataat attttctatt gtttaataaa gtattcataa ttatctctcc
      301 cttttaatat tttaattagt cagaaaatta tatgtattct cgacttgtta taaattataa
      361 attatacaat aataaaagtc aactgctaaa tttaattaaa atactaaata atgtatactc
      421 agaatacttt taaattaatg tattgtttgc aaaaaaacgt tatcctaagt tataagtgaa
      481 tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt atatcaatca
      541 aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag ctcaagtagt
      601 gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt ttatggaaaa
      661 tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc aaagggttag
      721 ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata ttacctattt
      781 tttgaaatta ataggtttta ttttgcagat tttgcatttt catctgactt tatatacttt
      841 atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc gttaattatt
      901 atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc ttctaatact
      961 tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc acaacaagct
     1021 aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt gttttcatta
     1081 gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg gacatataaa
     1141 acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga agagctgatt
     1201 cctttttcaa atacttcata attatttttc ttagcataat catgtagaga atcaacttgg
     1261 atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc ttttggagtc
     1321 aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt accagaaaca
     1381 tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc tgtaacagca
     1441 gtcattatta tcggaatatc tttagtagaa ttataagctg cttgtgcaga tggagtagat
     1501 atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact ttgtgtagta
     1561 ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc tttatagcct
     1621 ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagatgg atgttccact
     1681 aattgactaa tacctatatt tttttgtttt ttgctatctg tttctttatt ttcatttgaa
     1741 gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag tactcctgct
     1801 ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct taaaatttat
     1861 taattatatt tgttcatata ttcatatgat agattataaa tatttctgat taaaactaag
     1921 attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac tgttaaatct
     1981 tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat tatcaaccta
     2041 tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt tatatttttt
     2101 tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg gtcaagtgct
     2161 gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat aattagtgat
     2221 agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata tttttttaaa
     2281 tccaaagaaa ttccttcaag taagtgttcc aagtcattcg ttttatgacg aagacatttt
     2341 tttaagttca ataatttccc tttattaagt gctagagata aattttctct aaccgtcata
     2401 gaaggacaag ttccaaggct tgggtcttga aaaactctac ttactatttg agttctttta
     2461 tactcagcta aatttgttat agtagtacta tctaaagtaa tttgaccaga agattctttt
     2521 ataagccc
//
LOCUS       DAKBNN010000324         2506 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000324 DAKBNN010000000
VERSION     DAKBNN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.330"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            1521..1910
                     /locus_tag="OUB02_003768"
     CDS             1521..1910
                     /locus_tag="OUB02_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCT8887483.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT         1095 a          180 c          391 g          840 t
ORIGIN      
        1 ttatttctaa aaatactaca caaaaactct actttagtag aacttaatgg cgcaaaatac
       61 ttcaatttaa aacatttatc gtactttaaa taaataacta taataatata cttacaatag
      121 ttacatagaa gctattataa ctacacagaa cagcgtataa ttaaaacttt tttaggatgt
      181 cattatattt tttcttaaaa ttaaaaaaca ctataccata attctatttt agtagaattt
      241 ttttgtcaat aataaaatta aatattaact aaaaaataaa aaaaatggaa ttatctaaag
      301 aatttaataa ccttacaata attccaaaat attatttcta tgaaaatttt gataccatga
      361 gtaactcagc cataagcgca taaagcacaa gttactcttt actttaataa aacttgacaa
      421 gaaaagaatt tatccttatt ctactctaat agagttaatt tgtcaataaa gaataaaatt
      481 attttcaaaa tattttcatt cttcattctt atctgttatt attttataaa atatcactta
      541 attagaggta attttagtat tgtttttagt ccctgactat caatgctcgt aatatctaat
      601 ttacctctat gtgtaagtaa gaaatttagg aagtgggaag tttagttata agtagtagta
      661 agtaagaaat ttagtaagtg ggaagtttag tagtaataat ttatagcaag taagaaattt
      721 aggaagtagg aagtttagta ataatttgta gcaagtaaga aacttaggga gtaggaagtt
      781 tagtaataat ttatagcaag taagaaattt agggagtagg aagtttagta ataatttgta
      841 gtaagtaaga aatttagaaa gtaggaaatt tagtaataat ttatagtaag taagaaaatt
      901 agggagtagg aagtttagta ataatttata gtaagtaaga aatttagaaa gtaggaagtt
      961 tagtaataat ttatagcaag taagaaattt agggagtagg aagtttagta ataatttgta
     1021 gcaagtaaga aatttagaaa gtaggaagtt tagtagtaat ttatagtaag taagaaattt
     1081 agtaagtgag aagtttagtt ataatttata gtaagtaaga aatttagtaa gtgagaagtt
     1141 cagtaataag ttgtagtaaa taaaaagttt aggaagtagg aagttcagta ataagttgta
     1201 gtaaataaaa aatttaggaa gtaggattta ctgaaataca aatatttaaa atcaaagagt
     1261 ttaaaagaaa ggagagctac tggacaagta tccaaaatag atagctgagt acaaaatatt
     1321 tcataaaatt aagcaatata atacaagaga attttgacat tatttatata tatttaccgt
     1381 gtacaaatag ctaaatttag gattaagatt gaaaaatatc ttataagtct gagcttatat
     1441 aaatccgtat aagatgtatt taatcgaaaa attttattat ggatattgct tgtctatatt
     1501 ctgagtagca aggttttatc atgactataa aaaagttgcc acaatcagaa ttaaagataa
     1561 tgaaatttat atggaaatca gattccaaag taacatctag agatattgtt ttaggaatgg
     1621 agcaaaaata tcaatggaaa caaacgacaa ctttgactct tttatctaga ttagtagtaa
     1681 aaagatttct aaattctcaa aagatagata aatatacgca ttatgaagtt ttaattaaag
     1741 aaaaggaata cataggagtt gagacaagag atttttttag aaatattcat gattcttcta
     1801 taaaaagttt attgttgtca ttacatgaaa atattaattt gagcaaagat gatattttac
     1861 ttattgaaga gtggataaaa aatctaaaag aagaggagaa agacgtataa gcaaacgtat
     1921 ttcttatagc atcaatataa agtgtatgta agtgtattta tgtaatatat tagtctactt
     1981 aagtaatatg acaaatgatt ataagtggat tatgatacta taatgtgaat ctaaataaat
     2041 taaatataaa ttaatagtgg atattctatt taattaaatt taaaaagcta cctttactta
     2101 taagtataat taaggtagtt ttttaaatag aggtagtgta attaaagaga atttttaaat
     2161 ataaattgtg aagctttaat atagatgata atatatattt tgcaaatatg acacagtgtt
     2221 ttcgtattta agatgataaa ataaatagga attaatgatg atattgaaaa aataaaaata
     2281 aaggattaat ataagatatt aaagagatag agataaaagt gttaacagaa gatattgaaa
     2341 aaataaaaga ttgatataag atactgaaga ggtagaaaca aaagtattaa tagaagatat
     2401 tgaaaaaata aaaacaaaag attgatagaa agtactgaat agatagagat aaaagtgtta
     2461 atagaagata ttgaaaaaat aaaagattga tataagatac tgaaga
//
LOCUS       DAKBNN010000325         2501 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000325 DAKBNN010000000
VERSION     DAKBNN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.331"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..675
                     /locus_tag="OUB02_003769"
     CDS             <1..675
                     /locus_tag="OUB02_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8887484.1"
                     /translation="VYKIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGH
                     GGSDTGTTGKPLGGIKEKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALS
                     NSLRPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRG
                     AKTRTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKG
                     IEDSLK"
     gene            1180..>2501
                     /locus_tag="OUB02_003770"
     CDS             1180..>2501
                     /locus_tag="OUB02_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HCT8887485.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDN"
BASE COUNT         1041 a          296 c          417 g          747 t
ORIGIN      
        1 gtatataaaa taggaggagg attgacttct aaggtaatga actctatagc atcatcatta
       61 tctaaacaca atacaactcc aacagaacca ggaaatagtg ggggtaagac agttatgatt
      121 gacccaggac atggtggttc ggacactgga acaacaggta aaccattagg gggtattaaa
      181 gaaaaagact atacattaaa tacttcactt gcgacaactg aatatttacg ttcaaaggga
      241 ttcaatgtaa taatgacaag agacacagat aagactttat ctcttggaaa tagaactgct
      301 ttatctaact ctttgagacc agatttattt acgagtatac actataatgc atctgataca
      361 actgggaatg gtgtagaagt attttataag cttaaagata aagatggagg gactactaaa
      421 actgtagcta caaatatatt aaatagaatc ttagagaaat ttaatctaaa aaatagaggt
      481 gcaaaaacaa gaacactatc aactgaccct acaaaagatt atttatatgt tttaagaaat
      541 aatgatatgc cagctgtact tgtagaatgt gcatttttgg ataatgagaa agatatgagt
      601 ttacttaata catcaaataa agtaaaagaa atgggtacac aaataggtaa aggcatagaa
      661 gattcattaa aatagtctac aatgagagca tagaaaaatt taaaataaat taatctgcaa
      721 ttttaaattt aaactaaagc tataagataa gaatgtaatt ataatagtaa taaagactgt
      781 atatacattg tgcatataag tgcgaaatga ggtatataca gtctttttat gattaattgt
      841 aaaaggattt atattatatg aagaatttac atatcttaag gctacagtta attgtcaaga
      901 gttggagtat tcatttgtaa tacacaataa aagcttaatt actatatttt attgtgtgtt
      961 tgtgtgtttt ttgtggattt ttatgtcaat aaatgatata gtatagataa ttaaatgtat
     1021 taaatatatt taattttttt attttttgag ttttataagt ttatttttat atattactta
     1081 tatattattt atattttggg ttagatacac tttgacatat tttcaaaagt taaaagatta
     1141 aaaataaagt agaaatttat gttaagtaag gagattaata tgagaagaaa tacaaaatta
     1201 ttaacaacag ggattctttc aatggcaatc gtcgcaccta caatggcatt tgctactgaa
     1261 tctaatgcta tggaaaataa cgctgattta aatataaact tagagaaaaa aagtatcgtt
     1321 ttaggtagca aatcaaaagt tagtgtcaaa tttaaagaaa aaccagatgc agatagcatt
     1381 acattaaagt ataaatgcta tgacatgcca ttgaatacaa ctctaaatta caatcaatca
     1441 actggggcat atgaaggaac tatcaattat aataaagacc cagaatatct aaatgtttgg
     1501 gaactacaag ggataacaat aaacagcaaa aataatccta aaactttaaa caaacaagaa
     1561 ctagaaaaga tgggattaaa tttaaaagac tataatgtaa cgcaggaatg tataattgaa
     1621 gatataactt ctagaaaaga tgtaaataaa tatttaagaa aaacttctgc acctattaca
     1681 gaacttacag gaagtgatag atacgaaaca gcagttaaaa taagtaaaga aggctggaaa
     1741 aatggttcag ataaggtagt tataataaat ggagatgtaa gtatagatgg cattatatca
     1801 actccactag caactacata taatgcgcca atacttttgg ttgaaaaaaa caatgtacct
     1861 aatagtgtaa aatcagaatt aaagcgttta aatcctagag atgtaattat aattggagat
     1921 gagaatgcta tttctaaaac tactgctaat caaattaaat cgactgtaaa tgctagtcaa
     1981 acacgtctaa agggctctaa tagatatgag acatctttat tgatagcaaa ggaaatagat
     2041 aaaaatcatg atgtggaaaa agtatacata acaaatgcta atggcggaga agtggatgca
     2101 cttactatag cagcaaaagc aggtcaagac aagcaaccaa ttatattaac tgataaaaat
     2161 agtattacag acaacacata taaatggtta aagagtgagg atttacaaaa tgcttatttt
     2221 ataggtggtc ctcaaatgat atcaacaaat gttataaata aggtaaatga tataactaaa
     2281 gataatgtta ctaataatag agtatatgga gcagatagac acgaaacaaa tgcaaacgta
     2341 ataaaaaaat tctatacaga tgatgagtta gaggctgttt tagtagctaa atcagatgta
     2401 cttgttgatg ctttagcagc aggtccattg gctgccaact taaaatctcc aatacttata
     2461 acaccaaaga cgtatgtatc tgcataccat aaagataatt t
//
LOCUS       DAKBNN010000326         2445 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000326 DAKBNN010000000
VERSION     DAKBNN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.332"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..841)
                     /locus_tag="OUB02_003771"
     CDS             complement(<1..841)
                     /locus_tag="OUB02_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCT8887486.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTK"
     gene            983..2410
                     /locus_tag="OUB02_003772"
     CDS             983..2410
                     /locus_tag="OUB02_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCT8887487.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRSFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
BASE COUNT          841 a          334 c          305 g          965 t
ORIGIN      
        1 acttagttgg agatatatta taattccttt taaataagcg actaaaataa tttatgctat
       61 caaagccaca tctcatagca atttctgtca tatttaaatc tgtttccttt aaataatata
      121 tagctttatc aagcctgatt ccatttataa aatcgattgt agtcttacct gttatttgct
      181 taaatgttct acaaaagtga tgtgcactca agttcacacg actagcaagt tcttttaatg
      241 taattttatc catatatttt tcttctatca aatcaaagat acattcaaat ttttccaaag
      301 aatactttct ttttataaat tcatcttcag aatatatttt atcaatatga cttcttaata
      361 agagtactat taattgatag atattagctt ttatagataa ctcatatcca acttctttat
      421 tgtgatattc atcaagtata gttgtaatac attttaaaat cagagaatca ttttgaatta
      481 aattattaaa tacaatatga tttaaagcca atggtgctaa gtacttggtt tgtaacaaat
      541 caacttgatt actaaaaagg aatgtgggtt caatacgaat actatagaat tttaaattat
      601 ctgataagct ttctaaataa tgtaattcat tactgtttgc tattacaata tttccaggca
      661 ctaccttaaa attatccaat ccacattcaa gatatgctat accatcaata aaataatata
      721 tttgtagatg ctcatgccaa tgtcttttaa agtttgctcc ttttttaatg tcatccttta
      781 aaaaaatatc tataaagttt tcttgacctt tcctgttaaa atatgtattt ttatccttca
      841 ttattccccc ttcatcaaaa tagtgctaat tataagcaat atagttattg tattcattat
      901 ttcatatcgc tattatataa aatgtaacta tcattgtaaa cattaacata atgccagtca
      961 ttataataag gaggtaataa aaatgacaaa ctcttttaaa aaagatgata atagaaaaaa
     1021 aatcacagtt ttatttgtag tcattgccat gacttttatg gcaacattag attctagtat
     1081 tataaatgtt gctctcccag ttcttgcaag caaactaaat gtatctctag catctattga
     1141 atgggtaata gcaagttact caattattat ttgttcaaca ctattatttt ttggaagact
     1201 tggagatatt attggtaaat caagagtatt tcaagtaggt actattctct ttacatcagc
     1261 ttcattacta tgtggattaa gtaattcttt aactttactt atagtttgcc gctttattca
     1321 aggaataggc gcttctgcct acatggcaaa caatcatgga attatcacag aactatttcc
     1381 aaaagaaagc agaggaaaag ccctagggat tttagttact gcagttgcta ttggaaatat
     1441 ggttggacca tctgtgggag gatttatttt gtcaatattt gattggaatg ttatattttt
     1501 tataaatatt ccaatcggtc ttattgtaat ctttttaaat acaaaatttc ttcctaatag
     1561 taaaaaatct tcagaaaata tggataaaac tggtgctata cttcaatttt taggaacaac
     1621 attatttttt agtgctctaa ttagtgctca acaaacagga ttactaaatc catatatatt
     1681 aattgcacta ttattatcaa ttatttttat tatattattt ttgatattag aaaaaaaaca
     1741 tcctcaaccg cttcttgacc ttgaaatttt tagaagtttt aaattttcat tgaatttaat
     1801 ttgtgctctt acatcattca tttgtattgc atcatcaagt attttaattc cattttattt
     1861 acaaagtact atgaaacttc cccctattca agcaggttta tttatgatac tatcaccttt
     1921 gattttagcc attttttcac ctatatttgg gaatatatca gataaaatta agtcagaaaa
     1981 aattatttta attggtcttc tcgttatgag ttttggattc tttctaatgt caagattgaa
     2041 agaaagttct gcccttattt tatttgtaat ttatatatcg ataatatcaa taggacaagc
     2101 tatatttcaa cctgctaaca atgctttgat aatgtcaagc tgttctagaa gtaagctagg
     2161 agttgtagga agcataaatt cccttgtaag aaatttagga caagttatag gtattactat
     2221 ttctacaaca cttttatata attttatgag tattaaagct ggatataggg tcaatgatta
     2281 tgtgattaat aatgataaaa tatttgtatt tggaatgaga aatgtttata ttattgttac
     2341 acttgtttgc ttaataggtg caatattaat tggcttttat ttatttaaat ataataaaaa
     2401 tgaacaatag tttttataat tatattcaaa ttatgattat attat
//
LOCUS       DAKBNN010000327         2420 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000327 DAKBNN010000000
VERSION     DAKBNN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.335"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            95..250
                     /locus_tag="OUB02_003773"
     CDS             95..250
                     /locus_tag="OUB02_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887488.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            380..1393
                     /gene="aroF"
                     /locus_tag="OUB02_003774"
     CDS             380..1393
                     /gene="aroF"
                     /locus_tag="OUB02_003774"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCT8887489.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1415..>2420
                     /locus_tag="OUB02_003775"
     CDS             1415..>2420
                     /locus_tag="OUB02_003775"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCT8887490.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     K"
BASE COUNT          978 a          244 c          440 g          758 t
ORIGIN      
        1 gaaaatattt atagaaaata ttgacaatta tattttcttt gttataatta ttaaacaaga
       61 agaataataa attataaaaa tggaggataa tactatgaaa ggaatattaa aagaactaac
      121 agcaaaactt tatagctttt tttactttat gttctggggc ttttatttta gcgctggcta
      181 tttcttttac aaaaatatat gtacttcctt tctggtgagg ccttatttta gtgattttga
      241 gcataagtaa attttataaa aatgattatt ttatatattt atataagatt tagtgcttga
      301 tagattattt tagagaactc attagtgagt tctctttttt attttaaata taaaattata
      361 atattgggag gtagtaaaaa tgatagtagt attaaaaatg ggagcagata aaaatgaagt
      421 taaaaaatta attgaggcta ttgggagaga aggagtagaa gttaatccca tagatggaac
      481 agaattaact gttttaggtt tagttggaga tactagtaaa atagatgcca aaagaataga
      541 agcaaataag gtagttgaaa aagttatgca tgtggtagag ccttttaaaa aggccaatag
      601 aaaatttcat ccagaacctt caatcataaa tgttaatgga atggaaattg gttcaaagaa
      661 aattgctatg atagctgggc cttgttctgt tgaaacagaa gaccaaatag tatctatagc
      721 taaagatgtg aaaaaatcag gtgcaggatt tttgagaggt ggagcattca aaccaagaac
      781 atcaccatac gctttccaag gattgaaata tgatggattg gatttattaa aaaaagcaaa
      841 agaaaaaaca gggcttccta tagttacaga aataatgtca acacaagata tagatatatt
      901 tgaagaaaat gtagatgtaa ttcaagttgg agctagaaac atgcaaaact ttgatttatt
      961 aaaagaatta ggtaaaacaa ataaaacaat acttttaaaa agaggattat ctgctacaat
     1021 tgaagagtgg ctaatgtcag cagagtatat aatggctgga ggaaatgaaa atgtagttct
     1081 ttgtgaaaga ggtataagaa cttttgaaac atatactaga aatactttgg atttaagtgc
     1141 aattttagct gtgaagaaac ttagtcattt accagttata gttgacccaa gtcatgcagc
     1201 aggtaaatca tggatggttg actctttatc taaggcagca attgcagttg gagcagatgg
     1261 tctaataatc gaagttcata atgacccagc acatgcactt tgtgatggaa aacaatcgat
     1321 taagccaaat gaatatgatg aattgataag tgaattaaag actatcgcat cagcagttgg
     1381 tagagaaata taatctataa aaggatggtt aaacgtggaa gaaaaactat tagtaaattt
     1441 aaaagaaaat agctacaata tatttattaa aaaaaatata ctaaagaata taggagaaga
     1501 aataaaaaaa atatattttg gaaataagat ttttattgta actgatgaaa atatagcaaa
     1561 attatacttg gataaagtaa aaaataattt aagtgaatct ggatttgaaa ttaaatcttt
     1621 aatattaaaa tctggtgaag aaacaaaatc atttaatact cttcctaaaa tatacgatag
     1681 tttacttgac tttaagttaa ctagaaaaga tttgattatt actttaggag gaggggtaat
     1741 aggtgattta ggtggatttg cagcagcaac ttttttaaga ggtataagtt ttattcaaat
     1801 tccaacatct ttattagcac aagttgatag ttctgtaggt ggaaaagttg gagttgacct
     1861 tgagagaggc aaaaacttag tgggaagctt ttaccaacca aaggctgtgt ttatcgaccc
     1921 aaatgtatta ttatcacttc cagaaaaatt ctatagagat ggaatggcag aagttataaa
     1981 atatggatgt ataaaagaca aaactttttt caataaactt ttaagtttaa aaaatagaga
     2041 agaagtaatg caacatatag aatacattat acataaatgt tgctttatta aaaaaactgt
     2101 agttgaagct gatgaaaaag actttggaga tagaatgctt ctaaactttg gccatacatt
     2161 aggtcatgct attgagaaat actacaattt tacgggttat acccatggag aagctgttgc
     2221 cataggaatg tataagataa ctttagaaag tgaaaagata ggaattacaa aaaaagggac
     2281 atcagaactt ataaaatcaa ttttaatgaa ttataatttg ccttatgaga ttgaattaag
     2341 agataataaa tctatagtag atgctatatc tcttgataaa aaaaatctag gtgatacatt
     2401 aaaaataata cttttaaaag
//
LOCUS       DAKBNN010000328         2419 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000328 DAKBNN010000000
VERSION     DAKBNN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.336"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1686
                     /locus_tag="OUB02_003776"
     CDS             <1..1686
                     /locus_tag="OUB02_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HCT8887491.1"
                     /translation="IYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYENIIL
                     NIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFFDNR
                     EFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFKLLT
                     FRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGISSD
                     VLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKRSRV
                     RIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIENLY
                     KKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIIDITE
                     RIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGEILV
                     GIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKSLIE
                     IQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            1795..2403
                     /locus_tag="OUB02_003777"
     CDS             1795..2403
                     /locus_tag="OUB02_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCT8887492.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
BASE COUNT         1062 a          193 c          409 g          755 t
ORIGIN      
        1 atttatttgt taagtttaag taataataaa gatgaaagca attacttaat aagtttaaat
       61 gatattacaa atgaaatcaa aatagatgaa gaaataagaa atataaataa agactatgaa
      121 aacattatac taaatattcc atctcctata cttgtaagaa gtgctgaagg tggcataaag
      181 aagaataagg taataagtat aaataaaaaa tatgaaaaga gttttaaatg tgtttcaagt
      241 gatttagtcg atatgacatt agaacaatat tttgaaactt ttaacataga tttctttgac
      301 aatagggagt ttaaaagatt aaatctaaca caagaaaaga aagctgaaat agtaagttac
      361 aatgacacag caaactgtat agttaatttt gtgatgtctg actcagaagg tgaagagcat
      421 gttgaagaag ttagagttgg agactattgg tcagataata aactctttaa attgcttaca
      481 tttagagata taactaaaga gataaatatt cttagaacag taaatgaaca aaaaataata
      541 tatgaaaaat tactggatgt tataccagag gctatttttc ttgaggattt aagtacatca
      601 agagtactat atacaaatag agcatttaga gagttgttcg gaatttctag tgatgtatta
      661 ggagttacaa cacaaaaata tagaaatata ttagtaaaaa aatacataaa caatttaaat
      721 ataggagaaa gagagaagag tatccatata gtaaatgaaa ataatcacat taaagaagta
      781 aaaatgatat caagaacatt atactttgga cagaaaagaa gtagagtgag aattattaaa
      841 gatttaagtg tgcaaagaga gtctgaaaga cttaaaaaag cattaataaa acaaagacag
      901 tatgaccaaa tgaagatgga gttttatgct aatatatctc atgaacttaa gacaccttta
      961 aataatatat atagttcagt tcaacttatt gaaaaccttt ataaaaaagg aaaaataatt
     1021 gattttcagg atatactaaa agaacatata aaaataacta aacaaaatat gtttagatta
     1081 ttgagattaa tagataatat aataaatatt tctcaagtta agtcagacat atataagatt
     1141 aaagcagtta attttgatat tattgacata acagaaagaa tagttacatc tattagtagt
     1201 tatgctaagt ctaaaggaat agatttaatt tttgatacag atgaagaaac tgttatggta
     1261 gggcttgacc cagaatcgat agaaagaata atactaaata tattatcaaa tgctataaaa
     1321 tttactttag aaggaggaga aatccttgta ggtatatata aaaaagacga aacagtagag
     1381 ataattataa aagatactgg agtgggcata gacaaagaaa aattaaatga tatatttaat
     1441 cgatttaaac aaattgaaaa cagtggtata tcaaatgaat ttggaagtgg tataggttta
     1501 tgccttacaa aatcattgat tgaaattcaa aatgggaaga tttacataga tagtaaggta
     1561 ggagaaggaa ctaatgtaaa agttatattc ccaattaaag aggttgttga agaagtatat
     1621 gataattcaa attacaatga taatattgaa aagtttgaga tagaattttt tgatatatat
     1681 aaataataaa tgtctaaatt tattgtttat atatatcaca ttaataagag ttaattttat
     1741 ttattacttt agttgtattt gatgcgtttt aaataaattt tatgaggtga aattttggaa
     1801 aaggattttt ttagaaaaaa tggaattgat ttagccaaat caatattagg taaatatcta
     1861 ataaggaaat atgaaaataa agtaattgtt accaaaatca tagagacaga agcatacatg
     1921 ggtgtaaatg ataaaggagc acatgtatat ggcaataaaa agacagatag aactaagcca
     1981 ttatatttag atggtggaca tatatatgtt tatttaattt atggtatgta taattgcttg
     2041 aatttatctg caaatataga aaatgtgcca gagtgtgtat taataagagg ggtagagcct
     2101 ataacttctt tagatgagat atctatgaat agatacaaca aagcttacac tgagttgagt
     2161 aaatatcaag taaaaaatat tactaacgga ccaggaaaac tttgcaaagc tttaaaaata
     2221 gatagaagtc taaatagtaa gagtataatg ggtgaagaat tatatataag tgatttttat
     2281 tatgatgata aaggaaaaaa agtatttagt aaggatgagt tagatataaa aactagtaaa
     2341 cgtataaata tagattatgc agaagaagca aaagattttt tatggcgttt ttatatagaa
     2401 taagttttta tataggtaa
//
LOCUS       DAKBNN010000329         2390 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000329 DAKBNN010000000
VERSION     DAKBNN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.337"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(348..1313)
                     /locus_tag="OUB02_003778"
     CDS             complement(348..1313)
                     /locus_tag="OUB02_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCT8887493.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLI
                     NGLEIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYH
                     SKFGVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYM
                     PDALQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQ
                     SIADSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            1756..2124
                     /locus_tag="OUB02_003779"
     CDS             1756..2124
                     /locus_tag="OUB02_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HCT8887494.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          876 a          314 c          316 g          884 t
ORIGIN      
        1 tacttttaaa cgggttagac tgtaaatctg ttataagtat attttttaat tcctctgtaa
       61 gttcagaaat taaactatca cctatgtatt tttttatgat ttcctctcta atttctttta
      121 aatttagcaa tattagtacc tgctacttac atagtagctt ttcctttcct tttaatacta
      181 ttattcattt ttgaatctta tttagatata ttttaagatt aaaagtcttt agctatataa
      241 ttttttaccc atttttaact tatattaaca tattacaggc ttatatctcc accctgctta
      301 cattgaggat ggagtatatt gcctattttg ataaaaatga taggttgtta tttgcctatt
      361 cctttttctg ataatttttc taacatatct ttggtcattt tatctaaatc ataactaaac
      421 ttaaagttcc attcttttac tgcactacta ctgtctagag agttaggcca agaatcagct
      481 atactttgtc ttacagggtc tacatcatat tctatcacaa aatcaggaat atattttttt
      541 atagaatcag ctatttcttc aggagaaaag ctcatagcag taatattaaa agcatttcta
      601 tggatgagtt tatcagctgg agcttcgatt aaatccacta ttgattgaag tgcatcaggc
      661 atatacatca tatccatttt tgtaccttct gctatataag atttatatct cttatttttt
      721 aatgcctcat aatatatatc aactgcataa tctgtagttc ctccaccagg aggcgtaaca
      781 taagaaataa gacctggaaa tctaacacct ctagtgtcta ctccaaattt tgagtgataa
      841 taatcacata gtaattctcc agaaacttta gtcacaccat acatagtatt aggtctttgt
      901 aatgtatctt gaggagtcat attttttgga gaattttcac caaaagcagc aattgaacta
      961 ggtgtaaaaa atttacaatc caattctttg gctatttcaa gaccatttat taatccattc
     1021 atatttaagt tccaagcttc tagaggttta ctttcagcaa ctgcagaaag taaagatgct
     1081 agatgaatta tagtatctac atcatatttt tttgcaattt caaaaaaacg attcttatct
     1141 gtaacatcta atatctcaaa tataccaaat ataccagatt cacatactgg attaccttct
     1201 tttactctcc ttgaactagc gattacattt tgttctccat actcatttct aagctttatt
     1261 gttaattctg aacctatttg tcctaatgca cctgttataa gtattttttt caatttcaca
     1321 acctcctaag ggtttaattt atgtagtact acttattttt attttagcaa atcttttgtt
     1381 taaatacaaa gaattaaaga taataatata cgacttaatt gaatgaagtg cagtaagtgc
     1441 ctaaaaattt aaagctaata tataatggtc tattgtataa caataaaaga gtagattaaa
     1501 ttattatata tagattgtgt gactaagtca cagaaaatag agttaattta atatataata
     1561 aaagcatatc aagtaaagct ttaaattaaa gtaactttat ataaaaagta atataaattg
     1621 ataaaattat aaatttattt atagtgaata taggttggat tttatagata caaaaatagt
     1681 atatatgata tgtagaccat tagcatgttt aaagtagcaa aacatataaa ataaaatttg
     1741 aaaggaagta taaaaatgac aagaccatca atatatcata gccaaggata cacttgtgca
     1801 gaggcattaa taaagtctta taatgaggaa cacaacacag atataccaat ctcaatagga
     1861 agtggtatgg gggttggaat gaatgttgga agtgtttgtg gagctgtaaa tgctgctgtt
     1921 gttataatag ggtatttaaa tgggcgaaat agtaatttag atgaaaatat agcaaggaag
     1981 tattcaagag agctattgaa tagagtaaga aataaataca gctcagaaat atgtgtatct
     2041 cttaaaaaaa ataaagtaac ttgtgaagaa ataataaatt tcacatatga tgctctaaat
     2101 gatatgctaa aaaatcaaaa ataattctaa gaaacaagta ttttacattc gatagaatac
     2161 ttgttttttt atgatatttt taataagtat tagtttatta taatttttaa atcaatttta
     2221 ttatatttta attgaaacaa tctactttga aaattactaa ctgacaaaag aatatgatta
     2281 tagtatagaa aaaatgtaaa ttatttaata ttaagtgaga gtatatgttt acctatataa
     2341 aatcgtataa attaatggct aaatttataa attataaaga aatatatagg
//
LOCUS       DAKBNN010000330         2386 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000330 DAKBNN010000000
VERSION     DAKBNN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.338"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            429..1340
                     /locus_tag="OUB02_003780"
     CDS             429..1340
                     /locus_tag="OUB02_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCT8887495.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1515..2012
                     /locus_tag="OUB02_003781"
     CDS             1515..2012
                     /locus_tag="OUB02_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCT8887496.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
BASE COUNT         1029 a          162 c          419 g          776 t
ORIGIN      
        1 agacgataac aagtgagata ataataaatg aattgacata gcaataagta aattaagtaa
       61 gacgataaca aatgagatga taataagtaa attgatatag aaataagtaa attaagtaag
      121 atgataacaa gtgaggtaat aataagtgaa ttgatataga aataagtaag ttaaataaga
      181 tgataataag tggaattgat atagcaataa gtaaattaag taagacgata acaaatgaga
      241 tgataataag taaattgata tagtaataaa taaattaaga tgataatatc caaagtgaga
      301 aagataatag aaagtaaata aaatcatgta agaataattt agataagatt ctaagatttt
      361 gtttatttaa aaattagtat gattaattta gattaaataa atttaataat tttagtaggt
      421 ggtaatgaat gaaaataaat agattaacag aaataatagt aattctatta aataaaaaat
      481 tagtaacagc taaagagttg gcagataggt ttgaagtttc tacaagaacc atatatagag
      541 atatagaaac tctgtctatg tcgggagtac cagtatatat gacgaaaggc aaaggaggag
      601 ggatatcttt aatagaagaa tattctatag acaaggctat attgtcaaaa aaagataaag
      661 agagtttaat agttgcactg aaaacattac aggcgactaa gtatccagaa ataaattcag
      721 tagtaaataa aattggctca atatttggag agcagaattt ttcaaactgg attgaaatag
      781 actttactga atggggaagt aattttaatg aagatgataa atttacaaaa ataaaagaag
      841 ctattttaag gcgtaatact ataaatttta attatgtaaa tagtttgtca agccagacaa
      901 acagaactgt cgaaccactg aagcttatgt ataagagtaa gacttggtac ttgtatggat
      961 tttgtaaact aaaagatgat tttaggatat ttagaattag taggataagg aatttatcta
     1021 taaaggatga agtgttttca agaaagataa tagaagaagt atgtttgaat gattctaaag
     1081 taattaagga aaatactata acgttgaaat tgaggtttaa agaaaagatg ctttttagag
     1141 tatttgatga ttttaataag gatttaataa ctaaaaatga agatgataca tatgatgtaa
     1201 ttacagaatt tcctattggt gaatggattt atggatatat tttatcgttt ggtgacaatg
     1261 ttgaagtact tgaacctaaa gatgttaggg ataatgtaat aactagacta agggagttgt
     1321 ctaaaattta ttcattataa tataatgcta gattaaagat tttattaaat atataatatc
     1381 ttgcatttag tatataatat ctcactgagt ttaattatat taaaaatatg acataaaggt
     1441 gtcgtatatt ataaattata attgaattag ttcaaaataa aatatattaa taaaaacaaa
     1501 gtgaggtata aagtatgaat tatgaaatag tagagataaa tgagaaaata gttataggaa
     1561 tatcgaagga gactacaaat aaagatggac aagcggtaaa tgatataggt gaattgtgga
     1621 aaaaatttat gggcaaaggg atatataatg ctataaaggg taaaaagaat gacaagacaa
     1681 taggtctata tacagattac cagggtgatt ttacaagtcc gtatagcttt gttgcatgtt
     1741 gtgaagtaaa tagtaattct gataaagaga agaatttaga agaattaaac actaataata
     1801 ctgttggaga aagtattatt agtaaagtta taccagcagg taagtatgca aagtttgtta
     1861 tagtaggagg tcaaaaagaa gtaggagatt tttggtttga gttttggcaa atggattttg
     1921 ataggactta tataagtgat tttgaagagt atcaatgtaa tacttttgat acaaagaaac
     1981 aagaagttca tatttatata ggaataaagt agatttgtta ttactatatt gaatgaagta
     2041 ttgagtatag tacatttatt atagatagtt ttattattta taactataag cagtaaatct
     2101 gtaaaagatt tactgctttt ttagtaatta atagtatcag tatcactttg aatcttcaga
     2161 catattcttc atttaatatt gtttattgat aaatgatact tgtaaatata atgatttgtt
     2221 atttagttta aaatatgaaa aaaagtatta taaacacatt agaatacttc tggatttatg
     2281 ggtatacaat ttgaaaaata taaaaaataa tgtataaagc taaatctaat ttaatcgata
     2341 atacatatag agaaatataa ttaagtaaat aaacaactat ctatta
//
LOCUS       DAKBNN010000331         2383 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000331 DAKBNN010000000
VERSION     DAKBNN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.339"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(65..328)
                     /locus_tag="OUB02_003782"
     CDS             complement(65..328)
                     /locus_tag="OUB02_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887497.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            644..1219
                     /locus_tag="OUB02_003783"
     CDS             644..1219
                     /locus_tag="OUB02_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887498.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            1317..2315
                     /locus_tag="OUB02_003784"
     CDS             1317..2315
                     /locus_tag="OUB02_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HCT8887499.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT          960 a          214 c          372 g          837 t
ORIGIN      
        1 ttgagatata ttaataatta ttaatataaa aaagttccta gtttttagga acttttttat
       61 ttgattaata cagagtatga gtttttgctt cagctatagc tcttgctgca cttttaggtt
      121 tttcagtatt ttcttttttg taaaatttaa tcacttcttc taaatcttta ttttcaatat
      181 tatgtttttt gttttcaaga atataattta caattgattc atttatcaaa tctgtcaaaa
      241 tattattttc gtcattagtt atcttagaca tttcttgtaa taaaatttca ttaacttcta
      301 ctgtaatttg ttttgtattt tttatcatat caaccacctc ttaagaaaat tatatcatct
      361 caaaaaaata aattcaatat gttttaaaat tatagaattt taattcttaa attctaaaaa
      421 agagaaaatt agataaagta gttttagatt ttaaaagatt aataaaaaat atttaaagat
      481 aaaagcttta ttctatatga ttctaagata gaggatttat aagtgtttaa ggtgaattaa
      541 atataagaca acttcttaaa gcaaatgggt gtggtattta aatctaaatg tagacatata
      601 atattaaata tgctaatata tcaaaataat aaggggggaa tttatgaagc ttaaagaagc
      661 attgattaca tgtgttgtat gtagtgtatg tttaactcca ttattggtaa atggagaaac
      721 aaaaatagat gcagatcatt gtaaagaatc tatagtaaat atagatacta tagtaaagtc
      781 aggtaaaagc tttaattcca agtataatca aggtatggaa gattataaaa atagtgatga
      841 gtataagttg aaagtacaaa aggagaaact acaaaatcag ttaggtatta aaataaagaa
      901 gttagtacct ataacttttg aagttagtta ttacacaagt cttaatcaag aaaatggata
      961 tggagcgata actgctacag gagatgattt agaagatggt tttgtagcaa acaatcactt
     1021 aagttttgga actaagatta ttgtagatgg aaaattaaaa actgttatgg atagggggtc
     1081 tagtaagtat tttagtaatt ctaatgcaat agatgtattt gtgcccaaat ggagtaatga
     1141 aagtgaatca gagtactatg atagagtcaa taatatggga agacattaca aaaaaggcta
     1201 cattattgtt gaagaatgat gttctttgaa gtaatatgta ttgattgtag tatttaatat
     1261 atatataata aatataaaac tattagtaca atgagatata ttactaggag ggcaatatgg
     1321 aacaatcgtt tataaatgga aaagtaaact tattaataga aaattacaaa gcattaaatg
     1381 aagttaaagg ttcttggcaa atgggattga tacaacatag ttgtgcactt gcatttaccc
     1441 ttaagaacaa gagaataagt cctagattag tggaagaacg aatagagctt attaaaaaaa
     1501 atactggatt attctctaat tttagaggat ataatatgtt ttatatggca acactacttt
     1561 cgtttgaatc aaatccagaa agtagtttta agatgatttt agatatatac aaagaactta
     1621 aaagtgaaaa gttttggggc gatacatatt taccacttac agcatcaata gtttatgaaa
     1681 atagagagaa aatggattat cttacatgta tatcaaaaat gaaaataata tatgattata
     1741 tgaggaaaaa acatccattt ctaacttcta gtgatgacta ttgtaatata gcacttattg
     1801 caattcattc aaaaaattta gatgaagatt tagaatacat agaaaaatgt tatgaatttc
     1861 taaatgaaaa tggtttttat aaaggaaatg atttacaagc attatcccaa attcttttat
     1921 ttagtgatga tagaactatg cttaaatgta aaaagactat tgaacttaaa aaagcattta
     1981 aagaaaatga ttgtaaaatg aattattatg gatatccaat aataggagca atttcattat
     2041 tagattatag agaagatgaa ataatagaag agataaaaaa tgtttcaaat aaacttaaag
     2101 aagaaaaagg ctttggaaat tggtctttag gaaaaagtaa tagagtaatg attagttctg
     2161 ctattgtagc ttcaatttat gcagatttga tacgaaatga gaataatata ggctctataa
     2221 ctaataatat atttttaaat attgttgttg ctattcaaat tgcatgtgta atggcagcaa
     2281 caagtgcagc tatagcatct tcttcatcaa attaataaga tttattgaag ttggatatat
     2341 taaatttaat aatacaattt atatgtttaa aaagaagata gta
//
LOCUS       DAKBNN010000332         2379 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000332 DAKBNN010000000
VERSION     DAKBNN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.340"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(148..999)
                     /locus_tag="OUB02_003785"
     CDS             complement(148..999)
                     /locus_tag="OUB02_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCT8887500.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1048..2217)
                     /locus_tag="OUB02_003786"
     CDS             complement(1048..2217)
                     /locus_tag="OUB02_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8887501.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
BASE COUNT          738 a          434 c          250 g          957 t
ORIGIN      
        1 aattatatat gtaatacttt accaataaaa gctgatattt ttcatcaatt aaatgaaaaa
       61 aatacaccat ttacatataa ttaatataaa tttttcatat aacaaatatc ctaaacttat
      121 ctaaaataaa tttaggatat tttattttta tactctattt acagacccac acactttaat
      181 cttttctttc acaaaatcct ttactgattg aagccctgct aatccatact ttttagggtc
      241 tataacctca gaatcttcat ctaaaacttt tttaacccct ttactatatg caattcttaa
      301 ttcagttgca tagttaactt ttgcaatacc tctttttata gcttctttta taatatcatc
      361 atgtattcct gaacctccat gcaatactaa aggtattgaa actacatttt taatttctga
      421 taacctatct aaatctattt taggttcacc cttatacaat ccatgtgcag tacctatagc
      481 aatagccaat gaagatattc ctgttctatc gacaaactct tttgcctctt ttggatttgt
      541 ataatcatta ctattcccgc catctaaatc atcttctttt ccaccaactt tcccaagttc
      601 tgcttccact ggtataagag aaggactaca tgcttttact actgatttag atatagatat
      661 attattttca aagctttcat gagaaccatc tatcattatt gatgtatatc cacttcttaa
      721 agctttcatt gcaaggtcaa aacttgaacc atggtccaga tgcattgcta caggtatatt
      781 tactctttct gcagcaacct ttgcatttgc tagataataa tcaagacctg catattttac
      841 agtagatgga gtagtttgta atattactgg agaatttaac tcctcagcag cttctataac
      901 agcaattacc atttccatat tttctacatt aaatgctcct actgcatacc cttcatcttg
      961 tgccttttta agcattgatg ttgttgttac taatgccaca ttgataccct cccaaattat
     1021 ttagttataa tattttaatc ttatctttta atattcatat attgttacac cttttactac
     1081 tcgatttact gttcctgtag ctgatggagt atctggctta tttccaacct ttaccgatac
     1141 aaacaatgct atagtttgtg caaacaatat gtatggcatt gcaagataca catctggtaa
     1201 tttattatac ttttctttaa actcaaactt tgttcctgag aagttatttt cattttctac
     1261 tgacactgca caagtgaatt tagcaatttt atcccttttt atctcctcta aaacatctaa
     1321 atcatatttt cttgtatata aacagttaga tacaaattca aatacaagtg tattttcatc
     1381 aataaatgat tttggtccgt gtctaaaccc cataggagaa tcatacactg tagatatttt
     1441 tccggctgta agttctaaaa gttttagttg tgcttcttgt gtcaaaccac ctaatcctcc
     1501 tgaacctaaa tatacaactc tatcaaaatc tttatttatt aactcatgta tttcatcttt
     1561 tctatctata acatttcttc ccatttcaat tattgcattt atatatgact tttctacatc
     1621 atcttctaaa ctatcgaata ttaacatagc agataacatc atacacgaaa aacttcctgt
     1681 catggcaaag ccttcatcat ttgattttga tggcattaat aacaagtaat tattttcgtc
     1741 gtttttagtc atcttagcca gttctccttc tggattacaa gttatagcta aatgataaaa
     1801 attatctaca atcttattcc ctaaatttaa agcagccaaa ctttcaggac tatttccact
     1861 tcttgcaaat gaaattaata atgttggtat atcttttttt aagtaatcat atgggtttga
     1921 aactatatcc gtagtaggta ttgcttcaaa tatgtatttt cttatgtcat ttttattttt
     1981 aaggtaagga agtattgaat ttcctatgta agctgaagtt ccagcacctg taaatataac
     2041 tctaaactgc ccatgattat tacttattgt atctatgaat ctacttaatt tttctttgtt
     2101 agatttatat atttcataag tttctaacca taattcaggc tgttgtttta tttcattagt
     2161 tgttattatt gcacctaaat cttttagttt ttgttcttct aatttaaaca ttttcattct
     2221 cctttcatac ttgtaacgtt tacatttggt aatgaggtta taaccacttt aaactataaa
     2281 aaaatatact cttttataat tttttgctta gattttttag tactacttaa actcactata
     2341 aaatatacac tatagatttt aattaatttt atagtttat
//
LOCUS       DAKBNN010000333         2362 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000333 DAKBNN010000000
VERSION     DAKBNN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.341"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            164..481
                     /gene="trxA"
                     /locus_tag="OUB02_003787"
     CDS             164..481
                     /gene="trxA"
                     /locus_tag="OUB02_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCT8887502.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(688..1962)
                     /locus_tag="OUB02_003788"
     CDS             complement(688..1962)
                     /locus_tag="OUB02_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCT8887503.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVID
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENIFKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
BASE COUNT          844 a          306 c          333 g          879 t
ORIGIN      
        1 atatattaga aatatttagt cttataaata tttaatctta taaatctatt tatcataata
       61 ttaaacctat taaaaatagg cggttacaaa aaagtgccta attgaactgt tatatagggc
      121 gtagtatatt aatatcaaga agataataaa ggagatgtat attatggtaa aaataataaa
      181 taataatgaa tttataaatg aagtagaaaa taaagatggt ttagtagttg tagatttctt
      241 tgcgacttgg tgtggacctt gtaagatgct atcaccaata tatgaagctt taggaaatga
      301 gatggttgaa aaagcaaact tcttgaaggt ggacatagac caaagtatag aattagctca
      361 gaaatttgaa gtatctacag taccaactat gctaatattt aaggatggaa aacctgtaga
      421 tagattaata ggttttatgc ctaaggagaa tttaaaaaac aaaattgaat catatatgta
      481 attaaagtaa attgaagtaa ttaaaataaa tctcatatat aaacaaaaaa actcattgta
      541 atgagttttt tgtttgtata gtgtaaaata gtttgtatag ttacaaatga gtgtctaatt
      601 gctaatgaaa ttgactgttg atataatttg cttttttgtg aaaacaaaac tactctctat
      661 ttattgataa aaaagaatag aaaaagctta tttaaaagcc aaagttttaa tagcatgtat
      721 aaagctatcc acctcatcaa aagtattgta cattcctaag cttatcctaa caagacctac
      781 aggattttcc ctcataaatc tgacgtatgc ctcttttgga tttataccta ataatttttg
      841 tacataaata tctgcaccta ctactccagc tccagtttct attccaaagt tatcaactaa
      901 atattttgct acatctttgt aatacatatt acttatatta aaagatatat ttgggataat
      961 tacattattt gatttatctc catatattat tatattaggt attttactca tttcttcttt
     1021 agcatataaa aataattctc tttcgtattt tgaaattctg tttagaccaa ttttatttaa
     1081 agtgttcagt gcttttccca ttgcaagagt tccaaaatac tctgggtatc ctgcttcaaa
     1141 acgttggggt acgtttgcat atagtatttt gttatcattt acaaatttgg tagaacctga
     1201 gccatatagt acaggattaa atttttcaaa gaatttttta ggtcctacca gagctcctcc
     1261 atttagtgga gaataacatt tatgagcact aaaaacaaca aaatctatat gctcatcaca
     1321 ttcatgtggc atcattgaaa aaggtttatg ctgaattact tgcacaatat ccaccaatat
     1381 ctttgcacca tacttatgag ctattcttgc tatttcatat ataggagtgg ttatacctgt
     1441 gacattacta gctcctgtaa ctgtaacgag agatacatta ccagcataat ttttaaatat
     1501 attctctaat tgacataaat caagttctcc tttttctgta attccaacaa cttttgtagg
     1561 aaaattaact ttaaaaggca ggtaattagc catatgctcc attgctgtgg taataacaaa
     1621 tttatctgga ttttcatctt taaaaaattt tgctaagaag tttattcctt ctgtagcatt
     1681 tttgacataa ataacagaat ccttttcttc gtctccatca ataagtttga ggatagtaga
     1741 gcgtacattt tcatatttta aagtattttt ttctgagatt attcctggag cattaatata
     1801 agtaagccaa ggaatctcac agtttaagtt atctattacg ttttttaaaa ctaatggagt
     1861 ggcagagttg ttaaaatata ttcttttaac agtttcacca tttttagttt taactttaag
     1921 atttgcacct tcaatgtatt tagatacatc catgaattgc ataaaaaaac cccttatcta
     1981 ataaactatt ctgtttttta ttaaaaaagc tcaagtttta atcaagagct tgtttaatat
     2041 atgttatgtt tttataaggg gtttcgttaa ttaattcttg ttgagatgtt tattaaatta
     2101 cacgaaaatg agctacagtt gggggaaact gtagctcact tgattttggg ggagataggt
     2161 tgaacttatt gtttatccgt atcatatttt tagtatagtc acttatttta atttatatac
     2221 atttttttat aaattttaca taaaatgtta aacactaatt agaaataatt atgaatatga
     2281 ctaataaagt tgtaaaaata gtaaagaaat tgattagaac ataatggaaa atatattgta
     2341 tataattaga aaaaaagatt tt
//
LOCUS       DAKBNN010000334         2307 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000334 DAKBNN010000000
VERSION     DAKBNN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.342"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            103..1008
                     /locus_tag="OUB02_003789"
     CDS             103..1008
                     /locus_tag="OUB02_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HCT8887504.1"
                     /translation="MFFKDIELNSKKELDSYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1017..2201
                     /locus_tag="OUB02_003790"
     CDS             1017..2201
                     /locus_tag="OUB02_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887505.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
BASE COUNT          948 a          189 c          393 g          777 t
ORIGIN      
        1 taaacaatat aaatgaaatt aataccataa taaaattgga gttttttata tatacaaaat
       61 aaaattttta aaattaagca ataaaaaagg taggaggatt ttatgttttt caaagatatt
      121 gaactaaatt caaaaaaaga gttagactca tattttgatt tagtagatta tgaagcttgt
      181 gagtactgtt ttagtacttt atatatgtgg caacatgttt ataagacagg atattatata
      241 ggtgaagatt ttgccgtttt agttggggaa tacgaaggag atagcttctc aatattacca
      301 cttgctaaaa aagataaact tccagaagtt gtagattttg tccttgaata tttttctaaa
      361 aataataaaa aaatatactt aagaggtatt actactgaag tagtagagtt tttaaaagaa
      421 aaatatccag gtagatttga gtatatagaa gaaagagatt tatttgacta tatttatgat
      481 gcagaaagtt taagaactct agctggtaaa aagaatcaaa agaagagaaa ccatataaac
      541 tatttcttaa aagagtatgc aggtagatat gaagctaaat tattagataa agaaaacttt
      601 gatgagtgtt tagtactaat gaaggaatgg gaaagtaata aggaagaaaa taatgaattt
      661 gatgaaagta tggatgatga attaataggt atcaaaaaga tatttaacca ttatgacata
      721 ttaaaagata aggtaaaagt gtttggtgtt tatgtagatg gaaaattaga agcttttagt
      781 ataggagagc ttttaaatcc aaatatggca ttgattcata tagaaaaagc taatcccgat
      841 ataagaggtc tgtatccatt tataaatcaa caatttttgg taagtgaatt taaggacgtt
      901 gagtttgtaa atagagaaga agatttaggt attgaaggat taagaaaagc taaactttct
      961 tatcatcctt gcagatttgt tgaaaagtac agtgttaggg aggcttaata gactatatgg
     1021 agattaggta tgctaaagaa gaagagatag aaagtataaa agatatttgg agttattgtt
     1081 ttaacgatac ggagagtttt atgaagtact attttaatga taaatataaa tctgaaaata
     1141 ctgtagtagc tttagatgaa ggaaaaatta tttcttctct tcagctaaat cagtataagt
     1201 tattactaaa tagcaaggta tataatacat catatgtggt aggagtttct acacttccag
     1261 agggaagagg agctggatat atgaacaaag taatgaaatt tactttaaac gaattgtata
     1321 aaaaagggca attagtttct atattgatgc ctattgacta tagactttat agaagatttg
     1381 gatatgagca ttgttacgac cagattgaat atactattaa cacagatgat ttaaaaaatt
     1441 ttaagtcaag tggaaaaatg ataaagtcta atttatcaca aattgatgat ttgatacaaa
     1501 tagatagaac ttttttaaat gaggttaatg gtaatgtgtt aaaagatgaa cattattatg
     1561 aaaacttatt taaagagatt caaagtgaag atggattttt atacattcat gaaggaaatg
     1621 aaaaagatgg atatatagtt tattttcttc aggaagataa aatgtttgtc agagagttat
     1681 tttataaaaa tatagatgcc cttaaatcaa tgcttaaatt tatatataat cacaatactc
     1741 aatgtaagat agttactata tctactccta caatagataa gataaggttt atattagaca
     1801 accctaaaga tagtgatata aaaataaaac cttttatgat gggaagagtt ataaatgtaa
     1861 agaagtttat tgaagatata gatattgaga atgatattaa tagctcattt aatttattaa
     1921 tagaagacaa gtttatagat gaaaataatg gtctatttaa aatttctata cagaataaga
     1981 aggtatctgt agagcagtta gataaaaaag gtgcagaaaa gcctcaagaa gattttgata
     2041 ttaaattaga tataaatacc ttaacacagt tatcattttc ttatatagat gtaaatgaag
     2101 ctattttctt aaatgatata aaagatgttt cagaagaaac actagaaaca cttaattgta
     2161 ttttttctaa aaaaaataac tatataaatg aatatatata ggtagagtac gttaaaagat
     2221 tttaaaaatc ttttgacgta tttttttgtt tagataacaa tttatcttgt ttaaaaattt
     2281 tctatttgaa aataatctaa ataatat
//
LOCUS       DAKBNN010000335         2305 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000335 DAKBNN010000000
VERSION     DAKBNN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.343"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(88..1443)
                     /locus_tag="OUB02_003791"
     CDS             complement(88..1443)
                     /locus_tag="OUB02_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCT8887506.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          854 a          396 c          249 g          806 t
ORIGIN      
        1 tatttttatt attaattagt tataaatcaa tctaatacaa cataattaca gtagattaac
       61 ctataaaaca taataattgc ttactaatta tttatcatcc tcaacaagtc tacttaatgt
      121 cttatcagat aagaataata atatcgctgc aacaaatagt attactggaa ttatagtata
      181 tactttaatc atatcaaatt taagagtaaa tgcatatata tatccatatc ctttaccagc
      241 tataaaagta gcaaatgtcc atactcccat aagaacacct agaagcttct taggtgcata
      301 cttacttaca aaagagttac caagtggtga gaataacatt tcaccaagac ttaaaagaat
      361 accaaacatg attatccata gtatactagc tttagcagtt tcaggagctc caactcctct
      421 tgaaaattct gcaccaacaa gcattaagaa tgcaagacct aagaatatta atccaagacc
      481 tgtcttttta aataaactca tatctccttg tggtctacta gcaagtttaa accataatgc
      541 tccaagaact ggtcctaata ctatacatac aagtgaattt aatgagtcaa accaagcaag
      601 aggaacatca aatcctccaa cattcatgtt tacaaatgct gcaccataat cataaactgc
      661 aagataagtt agataccaga atacccagaa aattacagag aagaatgata taagtataat
      721 agcccaaatt ctcttcttat ccatgcttgt aagaggagac ttatcttttt cttcaatttt
      781 ttcagcaact acaccttctt taaaaggtct ctttcctgca ttcccaagga atctccatcc
      841 atatatgaac catattgcac ctattacaca aagtacagct gctataaaga aacattgaga
      901 aaatccaagt acaccatttt ttgcaaatgt tttaaggtaa agaataccaa ctgctatagt
      961 accaataaaa gaacctatat taacaaaaga atactgtact gaaaatgctg tatcaagttc
     1021 ttcttgacta tcaaaaagct gtccatttac cgcagatacg ttacctttaa agaatgcagt
     1081 accaattgaa actaagatta tcattgcatt taccatagaa actgtagttg caatagaacc
     1141 aagatagtat cctactccca tgataagcat acctataggt atagtatatc tagctcctat
     1201 ccatctatct gatatatatc ccccaattat aggtgcaagg tatgtgaatg caacaaggtt
     1261 ggcttgcatt atagcagctt gtcctttatc tattccaaga ccaccatgaa ctatagctgt
     1321 tgtaaggaat atatatatta agtactttga agcataatat gctgctcttt caaatgagaa
     1381 agttgttgag cagatataaa atcccatagg aaatttaccc ttctttttta ctgttgagct
     1441 cataatttta aatcctttct taattgtatt tttatttttt acgttttgtc caaattcttt
     1501 cttttatgaa agatataata ttattatttt gtaaaacaat ttgtaattat tattaagata
     1561 attgcgcatt tatttgtaga gtacatattc tcatggaata aaattaatac acttcttaat
     1621 cataaattta aattatataa attcactatt ttcagatata gttataaatt aatatattct
     1681 catctaatac catgaaaaaa ccttcgccat atgcaattct tattttttac gtgatagtat
     1741 tttacaaact aaaatccccc ttttgtagca acttatgtaa ttacatttta gtgctttttt
     1801 aaaggagtct cgaataaagc aacatatatt ttgttcaaat aatgttttta tttatttttt
     1861 aaatttatat attacttcac ccaaccaatt ctcaacttaa ataactaaac tactatctat
     1921 tcacagatta ggcttatttc ttacacattg gtttataata aacactgtga atctaaacat
     1981 aaaaactgtt attttaaagt aattacccat atattcgctt tgatactatt tattgaggtt
     2041 atactcttta tgtataatat attatttata aaatacctca aaatatatta ttcataaaat
     2101 acctcaaact gaaaactttt tcacgaatat ttatttttca cctccccttt tttatttcac
     2161 catgtaaatc gtaccataaa tatgcactta tatgcaagaa aaaaatctat taatcgacag
     2221 taaataatta gaaaaattca aattatattt atcaagtata ttatataaaa tatttcctat
     2281 gaaaatataa attaaattaa tgttc
//
LOCUS       DAKBNN010000336         2253 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000336 DAKBNN010000000
VERSION     DAKBNN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.344"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            216..590
                     /locus_tag="OUB02_003792"
     CDS             216..590
                     /locus_tag="OUB02_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCT8887507.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            587..1450
                     /locus_tag="OUB02_003793"
     CDS             587..1450
                     /locus_tag="OUB02_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887508.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1450..2088
                     /locus_tag="OUB02_003794"
     CDS             1450..2088
                     /locus_tag="OUB02_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCT8887509.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGIERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          854 a          231 c          322 g          846 t
ORIGIN      
        1 tagtatttcc aatttattgg caggattttt gaacaatatt tataaatttt ttaaaaaaat
       61 ttaaatctat ttaatatggc agactttcat aaaatacatt acatttatta aatgtataat
      121 attggcaatt agttttttct aaaatcagtt gacatattat atatatattt atataatgtg
      181 tatatactat atatacaatt taaggaggtg tcttattgaa tatagttatt agtaattcga
      241 gtgggaagcc aatatatgaa cagattacta cacaaattaa aagtatgatt ataagtggag
      301 agttaccaga aggctcagct cttccaagta tgagactatt ggcaaaagaa cttagaataa
      361 gtgtaattac tacaaaacga gcatatagcg atttagaacg agacggattt attgagacag
      421 ttactggtaa aggcagtttt gtatctagta aaaacatgga ttttgtcaaa gaagaacaat
      481 ttcgtttaat tgaagaatac ctacaaaaag cagttgatac agcaaaaagt agtgatatta
      541 catatgacga attaaaagat attttatttt tattatataa aggagaatga taagtatgga
      601 tgcaataact ataaaaaatt taaataaaac ttataaagat tttagcctac aagatatatc
      661 tttttcagta ccaaaaggga gtgtaatggg atttgtaggt gaaaatggtg caggaaaaac
      721 aactacatta aaagcaattt taaatttaat atcttatgat agtggaaata tagaaatctt
      781 tggtcttgat agtaaaaaaa atgaaaaaga aattaaagag caaattgggg ttgtatttga
      841 gggaagcaac tttcatgaaa atttaaacac tgaccatgtt tcaaaaatca tgagtaaaat
      901 ctataaaaat tggaatgaca ccttatttaa agactactta aaaaaattga gagtaccaga
      961 caataaatta attaaggagt tttcaaaggg gaataagatg aaacttagta ttgctgtagc
     1021 cttatctcat aaaccaaaat tattaatact agatgaggca actagttcac ttgaccctat
     1081 cgttcgtgaa gaaatactag atatattttt agattttata caagatgaag aacactctat
     1141 tatcttatct tcacatatta caagtgattt agataagata gcagactata ttacatttat
     1201 acataaagga aaaattgtat ttagtgaaaa taaggatgaa ttaattgata ctatgggagt
     1261 tttaaaatgt aaacctagtg attttgataa cctttcaaga gaggattatt catattatcg
     1321 taaaagccaa tttggatatg aagtacttct taaagataaa catagattta tctcaagaca
     1381 tccaaactgt attgttgata atacgtctat agaagaaatt atgttatttt atgtgagagg
     1441 tgataaataa tgaaaggtct aattttaaaa gatttactta atttgaaagg aaatataaag
     1501 tttatacttt tattcatcat tatgtttggc tttatgtctt ctttaggaga tggaaatgtt
     1561 aataacttta taggtgttat tatagtttta tgtactacaa tgattgtttc aacattttca
     1621 tatgatgatt tgaataaatg ggatagttat gtacttacaa tgcctattaa tcgcaatgat
     1681 attgtattaa gtaaatattt aactatgtta atatttagct ttataggtgt tcttgtttca
     1741 cttattgtta gtgtaactat tggttatttt aaaaatacac ttatattaaa tgaaacacta
     1801 cttattaatg cactaatctt atcaatttca gtatgttttg gtagccttat tctaccactt
     1861 atttataaat ttggtataga aagagctcgt ttattaatga ttttatgctt tttagttcca
     1921 acactagctt tattagtttt taaaagtatt ttagaaaata ttagttcacc tatttctata
     1981 gaaattatac ttaatacact agtatattct cttccatttg ttgctatttt actatttgtg
     2041 atttcatatt ttatatcatc aaaaatctat agtaaaaaag aagtataaaa tatactatat
     2101 atatttctgt atttaagtat ttaaataaaa tactatcgag tttattttgg tagtatttta
     2161 tttttcaact attaatctct actaggtatt tattatttta ttacttatat ttaataagac
     2221 tatttactac tttctaatct tatttatata att
//
LOCUS       DAKBNN010000337         2199 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000337 DAKBNN010000000
VERSION     DAKBNN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.345"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(34..207)
                     /locus_tag="OUB02_003795"
     CDS             complement(34..207)
                     /locus_tag="OUB02_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HCT8887510.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(648..863)
                     /locus_tag="OUB02_003796"
     CDS             complement(648..863)
                     /locus_tag="OUB02_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887511.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(874..1341)
                     /locus_tag="OUB02_003797"
     CDS             complement(874..1341)
                     /locus_tag="OUB02_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCT8887512.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFFDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          884 a          228 c          244 g          843 t
ORIGIN      
        1 tgtaaattca ctaaaagacc tttttataga atattatttt ctttttttat taaataggta
       61 aatcagtaaa aatgcaattc cagttccaat tattattcca atgatatttt ctataattaa
      121 tttttttaat ataaaagtaa ataataaaaa tgctatacat aaacctaaaa tatatccttt
      181 aaaataggtt gaaagttttc ttttcatata atcaccaact tgtatcagat ttagtttact
      241 taattagagt ataacagtgt tgtaacttca aagagtatct aaaagattac aaatgttaga
      301 taaaatcatc aataatgtaa tttataacac gtagctataa actatgcaat aatataaatg
      361 gaggtataag taaatgaaaa agttccatgt tttgctaaca gtttaaacaa atattaggta
      421 gaaaagaatg tatataggaa tctgtggtaa aattatcaat ttaaaagtat gatttagtgt
      481 gatacagttc ttataggtaa tggataacaa atagataaag gaacagtttc tttcttagaa
      541 catattttgt tatgacttta aacaaagtta atattaatgt caataatata gtaaaagaat
      601 aaaaatcata tagtataata aaaaagagtg tttctaaaca ctctttttta atcattttca
      661 tcttctacat attcaagtat atcacctggc tgacagtcta aagctttgca aatttcgttt
      721 agagtggtaa atctaatagc ttttgctttt ccagttttta aaattgataa atttgcattt
      781 gtaatatcta ttttttctgc caaatcttta agtgatattt tcctttttgc catcattaca
      841 tctaggttaa ctactattgc cataacatca cctctatatt gttaaatcat tatcttcttt
      901 tatttttata gctttatcaa atgtttctgc aattacaaag cacattagtg caaatataaa
      961 agagataacc ataagtggtg agataccgcc actatcagtt ccaaaaattc ttatattatt
     1021 agtatttaaa ttataaccat ttatacattc aattatagtg tttatcaata aacaacatcc
     1081 cattacctta aaccttttta cattttcaag tacgaatggg gaatacttag tactattaat
     1141 tataaatatc aagaataaga tgactataaa gtagtcaaaa aaatatagta ttcttgcaat
     1201 taaagcgatt actttgtcag aactaaaatc atataaaaac ataggtatca tacataagaa
     1261 tattatacca gcaactccta aaagtgataa tagaaaatat agaaatactg ataatatatt
     1321 actaaaaaat tttgtaatca taaaatatca tctcctttat ataaagtata ttattgatta
     1381 taatatataa tttattgaat ttcaataata aattattgat ataatatata aaaccattat
     1441 aatatatact aaaaataaga atgtataagg tggtaaattt atataaaaaa gaatatgagt
     1501 tgtaaaatat taaataatat tgtactaatt ggaataattt taactttatt atagattatt
     1561 ttaataccat tagtactgat ggtgtatttt aaaattggta taagcattac aaatacgaat
     1621 atgccaactg taatatgtaa tataagtagc tatatacata tgtgctgtcc catatattgt
     1681 atcattgttt atattaaaaa gattatgtaa attggtatca atcaataaat cattttcaga
     1741 agaaatacct aaaaacttaa aaaataccta tatgtgtatt tagttagatt tgatattttt
     1801 acatttaatt ttatagtgac aatattatat tatttttatg atgtctattt atatgcaatt
     1861 actataatat cagaacttat agtatcattt gtttcattat caataaggtt ttttacttta
     1921 gttttgtcaa ttttattaaa aaaagttatt gatataaaag ataagaagga taaaactata
     1981 tgacggattt caaacataag agtggatatt tatataatag atattttata gttaatttta
     2041 ttagatttta tttaatactg taatattaat atcacaatat attaatagtg tattaaagat
     2101 tgaattttgt agataactat aatttattga gagtgataaa taaaaattat ataatattac
     2161 agaagaaaga gagggagaca atattatatc cctcttttt
//
LOCUS       DAKBNN010000338         2182 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000338 DAKBNN010000000
VERSION     DAKBNN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.346"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            201..1514
                     /locus_tag="OUB02_003798"
     CDS             201..1514
                     /locus_tag="OUB02_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCT8887513.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1532..2068
                     /locus_tag="OUB02_003799"
     CDS             1532..2068
                     /locus_tag="OUB02_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCT8887514.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          866 a          204 c          416 g          696 t
ORIGIN      
        1 ttatatatat tggaaaaaaa tgtaaaataa tgataaaata tagtttataa tacaaaagta
       61 taattacatt ataattatat gtagttagtt ggatttgtgt atttaaataa tgtataattt
      121 atgctgatta ctgctaaaaa agcataataa tattgcattg taaggtttta taaacttcta
      181 attgaaaggg gataattcaa atggataaga gatatttaga atccgatgta tttgaagctt
      241 ttcaagaaag actttcttat ctttttaatg aatttgatta tatctatgta tcttttagcg
      301 gaggaaaaga tagtggttta ctgctaaatt tagttttgga ttataagaga aagaataata
      361 taagttccaa aataggagta tttcatcaag attttgaggc tcaatatagt ttgactactg
      421 aatatgttga aaaagtattt gaagataata ttgaagatat tgaaccatat tgggtgtgtt
      481 taccaatggc tacaagaact gctgtaggaa attatgaaat gtattggtat ccatgggatg
      541 atgagaaaga agagttatgg gtaagggaaa tgccagtcaa agaatacata ataaatcaaa
      601 aaaataatcc ttttacttat tataaatata aaatgagaca agaaagtttg gcaaaacaat
      661 ttggtagatg gattagagat atacatgaag gtgcaaaagt tgtttgctta ttaggaacac
      721 gagcagcaga atctctacaa cgttattgtt ctattgtgaa taaacagcat ggatataaag
      781 gaaagtgctg gataactaag gaatttaaaa atgtctggac ggcatctcct atatatgatt
      841 gggaaataga agatatatgg ttagcacact ataaatttga ctatgactat aataaaattt
      901 atgacttatt ctataaagct ggagtagctc caagaaaaat gagagtggca tcacctttta
      961 atgattcagc aaaagagagt ttaaatttat acagaatatt ggacccagat gtatgggtga
     1021 aattggttgc tagagtacaa ggagttaatt ttacttctat ttatggcaaa acaaaagcta
     1081 tgggatatcg tagtgttaaa ttgccatatg gacacacatg gaaaagtttc acatattttt
     1141 tgcttgatac cttacctgaa aatataagga atagctatat taaaaaattt tcaacatcta
     1201 ttgagttttg gcagaataca ggtggtggtt tgtcagaaat agttatacgt gaattgttga
     1261 gaaaaggtta taaaatagaa ttaaatggag tttcaaatca tacaaaagat aaaaagagta
     1321 gagtcatatt tttggatgat atacctgatg atacggatga tattaaattt accaaagata
     1381 taccaagttg gaaaagaatg tgttattgta ttttaaaaaa tgaccatatg tgtagattta
     1441 tgggatttgg tcctacaaaa gaacagagaa ttagaacgga atatattaag aagaaatatg
     1501 aaaaaattca ttagatagga gttaagatgc tatggaatat aaaagtcctg tatatggaat
     1561 catcgcagtg ccaatcgaaa agatagaatc taacgactat aatccaaatg cagtagcccc
     1621 accagaaatg cagttgttgt ataaaagtat aaaagaagat gggtatacaa tgccaattgt
     1681 ctgttattat aatgaggaaa aggataagta tattattgta gatggttttc atagatatag
     1741 agtaatgctt gaaaatgagg atatatatga tagagaaaat ggacttcttc ctgtttcagt
     1801 aattgataaa tctcttgatg aaagaatggc aagtactatt cgacacaatc gtgcaagagg
     1861 ttctcataat gtggatttaa tgagtaaaat tataactgaa ctttgtgaaa ttggaaaatc
     1921 tgatgaatgg atatcaaagc acctgggtat ggaagttgat gaaattctta gattgaagca
     1981 aataactgga ttagcttatt tatttaaaga taaagagttt ggtaatgcat ggattccctt
     2041 tggctctgat atagaagaag aaaattaaat agatttatta aaagaaaaag atttattaaa
     2101 aaagaaaatt taaaatatat attaaaaaat aaagagtttt aatattatat gtaagtacag
     2161 aaaaaagagt aggaaaatta tc
//
LOCUS       DAKBNN010000339         2173 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000339 DAKBNN010000000
VERSION     DAKBNN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.347"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..1899)
                     /locus_tag="OUB02_003800"
     CDS             complement(<1..1899)
                     /locus_tag="OUB02_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
                     /protein_id="HCT8887515.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDINNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSGGSSGGSSSSKVGQIKFSATDKQIKMASGDKTVDPSDNTYILKLTNGTVKDGDVKA
                     NVKVAGLPSGLDYTAVGNRSANTITITVSGTASQSIQNDLNNVSVLVKAGAVSGTGAT
                     DSIANTTFE"
BASE COUNT          568 a          394 c          271 g          940 t
ORIGIN      
        1 ttcaaatgtt gtatttgcaa ttgaatctgt agctcctgtt ccagatactg ctcctgcttt
       61 aactagcaca cttacattat ttaaatcatt ttgtattgat tggcttgctg ttcctgatac
      121 tgtaattgtt attgtatttg ctgatctatt tcctactgct gtataatcta gtcctgatgg
      181 taatcctgct actttaacat tagcttttac atctccatct tttactgtcc catttgttaa
      241 ttttagtata tatgtgttat ctgatggatc tactgtctta tctcctgatg ccatttttat
      301 ttgtttatct gttgcactaa attttatttg acctaccttt gatgaagaac ttcctccact
      361 acttcctcct gaagaacctc cacttgatga acttcctcct gatgaattag aacttggact
      421 atttccactt attgttggag aattatctcc cttaacacta cttattgtac ctttattatc
      481 tatagtagtc cctttagaat tatttgtaac tgtagttatc ttaccaccat tagtcacttt
      541 tacttgactt gctccagatg ctactgttaa actaccaatc tcacctttag aattattttc
      601 tatcttggct ccatttttat cattaacctt taagttagtt atttttccgt agttaacaaa
      661 agttccatct tttacatcat ctataactac atcatccact tttgcatagt tatttatatc
      721 accattaggc atgttgatag tcacagtctt tgtatatgta ccatttaagt ttactgttac
      781 tgctttatca gtttgtattg taaatgcttc tttaacctca ctagttggct taaagtttat
      841 aacatcatta gcagtagctt tatctatagc tgattttaat tcagaaattg ttttagcttc
      901 aactgttttt acttcttgta atgacttcat ttcattaaat gctgtctcat taccattccc
      961 accaacttta gttatcttat caaaactctt agagttaact agagattttt gtgaagctga
     1021 taagttcttt ccaactaaaa ctactggtga ttgattttta gcacctaata cacctactgc
     1081 tagtgcatct ataagttggt cttgtttatt cataccatct ttagttacat aagcattttt
     1141 taaatctgtc tttttataaa attcttttat tacttttgca tttgtatcat ttctatctgt
     1201 tccacctagt cttgtactat ttggtagttt atttttaaca gcttcagaaa ttgaactctc
     1261 tccacctact acataagatt gagttattcc agcatctttt ataaatttgt ctgcaactgc
     1321 tgttccattt ttagaatcag caagtattac tggcatattg ttttgtgcag ctggtgctcc
     1381 tacacttaca gcatctgcaa gtcctttttc accattaact acagctattt tcttaacatc
     1441 acttatagca tctaatttct ttgctaattc taaggatgtt tgatatctct cagctccaga
     1501 gattctctca aaagatattt ttaaatcttt tatttctttt tctatatttg tacttaatac
     1561 agaagttcca cctattaagt atactttttt agcttttaat ctttgtattt ctgctttcgt
     1621 cttatcattt aatttatctt tacttgttaa aagtattgga gcattttttg cttttgcaaa
     1681 tggtgttgca gatagagcat ctgctataga tgagtcgttt acgattacta cctcatcagc
     1741 atttttccat ccatcttgac ttattttaac agcagtttca tatctttctt gccctgttaa
     1801 atttgttgcc acagtttgtg cactagctat gcctgctgtt gaacttccag ctatcattgt
     1861 aactgtaagc attgcagcaa tcttctttgg tattttcatt ttattttctt cccccttgtc
     1921 catttgatat tacttaattt tttaactttg ttcccgttct aattctaaag gatacttaat
     1981 atctaatttt aaagaggttt ctaactataa tgatttcata attaatttgt aattattttg
     2041 ttacattgca gtcattatgt aaggaataaa gaaattttct tattgattga aaaatttgta
     2101 aatttatatt ttaaggtaag tttgattttt atgttaatga attgttataa aaaatagggt
     2161 tttgtataaa aaa
//
LOCUS       DAKBNN010000340         2164 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000340 DAKBNN010000000
VERSION     DAKBNN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.348"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            199..295
                     /locus_tag="OUB02_003801"
     tRNA            199..295
                     /locus_tag="OUB02_003801"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:233..235,aa:Sec,seq:tca)
     gene            446..1153
                     /locus_tag="OUB02_003802"
     CDS             446..1153
                     /locus_tag="OUB02_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCT8887516.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(1295..2131)
                     /locus_tag="OUB02_003803"
     CDS             complement(1295..2131)
                     /locus_tag="OUB02_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8887517.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENKKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT          805 a          241 c          311 g          807 t
ORIGIN      
        1 gagaagaaac tctttataga ttctaaaaat ataatttcca gttttttatt ttggatatgt
       61 aataaaacag gtaaatttta accatgtata gataaaagct atttaaatta taatttacta
      121 tcatataaat ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt
      181 tgagaaggat ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact
      241 agtatgaggg gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg
      301 taaaaggtat cttttaatta aaagatacct ttttaaattt attttaagat aatttatttt
      361 taataatata taatgttttg tattatataa tgtaaatttt ctaatactat tatatattta
      421 gataaaaatc taggagtgtg gaaagatgga aaataatttt acaacgagaa ctagttttct
      481 agttggagat gatgggataa aaaaacttaa taattcaaat ataatagtat ttggagttgg
      541 aggagttggt agttttactg ttgaagcttt agcaagagct ggagtaggca atattactat
      601 tgtagatttt gatgatgtag atataaccaa tataaataga caaataccag ccctacattc
      661 aactgtagga agatacaaag ttgatgtcat ggaagaaaga atattagata taaatcctaa
      721 tatcaatata aaaaaaataa ggagtcttta taataaagat actagtgacg aaatactaac
      781 tgaaagatat gattatgttg tggatgcgat agacatggta agttccaaaa tacatctaat
      841 tgaaacttgt gaaaaaaaag gattgaaaat aataagttct atgggtatgg gaaataaatt
      901 agacccaact aaaatagtcg ttacagatat acacaaaaca agtacttgtc ctctagctaa
      961 agttatgagg aaagaactta gggatagagg tatcaaaaag ttaaaagtag tatattctac
     1021 tgaacaacca atagaactta agaagaaagt aatgaatggt agaaaagtaa cacctggtag
     1081 tgtatcattt gtcccatctg taggaggttt aataatcgca tctgtaatag taaatgaact
     1141 attaggacaa taagttttaa atattttaat ttataaattt tatatacaaa tttgaaggct
     1201 agaagcatga atacaaagca attttatatt catactttta gcctaattat tttatattac
     1261 cttattttat gtagttgtag ttttattttt tagattatgt atctatttca taattatatt
     1321 ttgtaaatat aatttctcca tcactataaa aaacaatatc tcctattcta tctgttctat
     1381 aaattgatac atggttttgt tttagattat taataacctc cctatgtgga tgaccatatt
     1441 gatttttata tccacatgat attgcagcta tatctggact aatttttttt ataaattcta
     1501 aagatgaaga agaattactt ccatgatgac ctatttttat aaaattaata ttatttaatt
     1561 taaaagaatg taaaatttct tttttatttt cttcttcagc atcacccata aataaaaaag
     1621 aattatcatt aaaagttaat ttaaaaacta tagaatttaa attagattct tcttgtatat
     1681 atgatggact taatacatat atatttatat tgttatctat gtttaaaaca tcatttttat
     1741 agagatgctg aatagataag tttttatccg tacaagaatt tattagattt tgataagcct
     1801 cactatcagt tgattgttca ggcatatata tggagtttac attaaatttt tttataatat
     1861 tatcaagact tcctatgtgg tctgaatctg gatgagttgc tattataata tcaatagttt
     1921 ttattctttt ttgtctaaga taactaataa ttatattttc gctatcttca tcacctccat
     1981 caattaatat attcttattt gtgggtgttt gaactaaaat actatcacct tgtccaacat
     2041 caatcatatg tatagatagt agagattttt tgtcacagcc gattaaaagt gatattataa
     2101 taagtattga taaaaaaatt ttatttctca ataaaaaatc actccattca ttacatgttt
     2161 atat
//
LOCUS       DAKBNN010000341         2146 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000341 DAKBNN010000000
VERSION     DAKBNN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.349"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(553..753)
                     /locus_tag="OUB02_003804"
     CDS             complement(553..753)
                     /locus_tag="OUB02_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT8887518.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(979..1155)
                     /locus_tag="OUB02_003805"
     CDS             complement(979..1155)
                     /locus_tag="OUB02_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887519.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1210..1307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1548..1931
                     /locus_tag="OUB02_003806"
                     /pseudo
     CDS             1548..1931
                     /locus_tag="OUB02_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          860 a          267 c          281 g          738 t
ORIGIN      
        1 tttgcagtga gcgatatttg tgataaattg aggtttagca gttgaaatat aaggtattga
       61 gaatatatga taagtattat caattgcact attgcgcgtt cactgcaatt ttaagagcat
      121 tgtatatgtg taaacattgg agatgctaag tttattttgg gttttatatc aactatgtgg
      181 tatgtaaaat ttttattaca ttaaaaacaa ttctcctaaa aacacaaata attgtcacaa
      241 cacacagtcc tcatatttta caaattgatt ctaaagaaga aataattgtg ttagatatgg
      301 atgaaagtga taatgtatat aaaaaatagt tagaacttgg agaatacggg gtattaggct
      361 ggaccaatga aggtttatat ttacattaag agcactcttt ttataggagt gcttattttt
      421 ttgaaattca ttagcatata aactatcaag aacattactc aatatacctt atttacttca
      481 ccaatgatta tcttacatat attaacaata aaaaaagact ctaaaaagag tctttcccga
      541 aaaatctata tattataata tagttatatt ttctgcttga ggacctctag cacctttaac
      601 tatatcaaag cttacttgtt gaccttcttc taatgattta aatcctgaag tttgtatagc
      661 tgagaaatga gcaaacacat catctctacc ttccattgat ataaaaccga atcctttttc
      721 attattaaac catttcacta ttccattatt cattaaaaat actcctatta acctatatca
      781 agatatactt gatttaattg tgtattacca caaatactat tgtatcatag attaatagaa
      841 atgtctactt aattatattt tttagttaac attttaatta ttcttaatat taaaaatata
      901 tttaaacgtt ttacattgct atatattaat ataaatgtag ataaaactaa aaagggtttt
      961 ctagaaatca atgtctatct aattatactt acttaccaat accattactt ggtgtaaaca
     1021 agactctact acattttctc cgtactctaa tataaaatca tctaactgtg tatctaaaaa
     1081 attacaataa tcatacgaac tgctaatata agcagtcaat acatgtgtac atttatccat
     1141 tttatttcca aacatataaa ttattatccc cctaaaaaac tttcataaaa acaaaaagaa
     1201 cactatctct ttggcaactg gctgacataa ctcataagat atttatataa gtcttagtcc
     1261 ctatagcttt gcgtcactaa atttctctag ttttgccgat ttagttttat tctacaacta
     1321 aaataataca atagaattag ttattattca acaggattgt ttgaaagtta aataaattta
     1381 tagatatgaa cattatttta cacttaaaaa cagactaaac acgtagagta cctggtaaaa
     1441 attaaatata gtttttgttc taaaaagctc ctattataat ttaagctaga taaatttgaa
     1501 gaagtgacat aagaagaagt gacataaatt gaaagaagga atatattatg ctaaataaaa
     1561 aattaccaga tacagaatta aaaattatga aatgcatatg gaatactggt tataaaactg
     1621 taatatcaaa agatgttgca gatgaaatag aaaaaacata tggttggaag catacaacta
     1681 caattatact tttagctaga ttaaccaaaa aaggtttttt aatatcatag agaattggga
     1741 ggcatgtaca ctatacagta ttaataaaag agagagaata tctcaaaata gagggaaaaa
     1801 gagtttttag aggtttacat aacaatcctt tatcagaatt aatttcaaaa ttacatgatg
     1861 gagaagaaat aactgaagaa aaaataatag aaataggaag ttggataaaa agctggaaat
     1921 ataaagatta ataaataaaa ggtagctaat aatataagtg ctaccttttt tatgtggata
     1981 atattggtga taagttgtgg aaaacttata taaaaattgt ggataaagtg ttttgctgac
     2041 attttactga caaaagttaa aaaaataaat gttatatata ttgtaaaatg ttgaaaaata
     2101 gccatatata taaaatataa gtaacactat agataaatta ctgtct
//
LOCUS       DAKBNN010000342         2143 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000342 DAKBNN010000000
VERSION     DAKBNN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.350"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            424..717
                     /locus_tag="OUB02_003807"
     CDS             424..717
                     /locus_tag="OUB02_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCT8887520.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            698..1339
                     /locus_tag="OUB02_003808"
     CDS             698..1339
                     /locus_tag="OUB02_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCT8887521.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1583..1915
                     /locus_tag="OUB02_003809"
     CDS             1583..1915
                     /locus_tag="OUB02_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCT8887522.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          818 a          188 c          323 g          814 t
ORIGIN      
        1 tgttattata ataataaata tattctgtta tagttgtatc taattagtag atgtttttat
       61 gtacttttat acagtatatt tctcgtttca ttcttttcta tttaaatagg acttaactac
      121 tttttaaatt gggtttagct acttttaatt taaaattaaa agtattgtta tatatataag
      181 tacctcagtc attaggtttt tgctttaaca attatggtgc aatacacttt tgttaagtta
      241 aaaaaattag ataaagtaag attaaataaa agcgacttaa gtaaattgag aatcttaata
      301 tgaaaatttt caataaatat ttagaaataa ctatttagaa aaatttctaa atagctattg
      361 aaataaaatg gatgttatgt tatattctat ttagatgaaa ttctaaatag cgaggtgatg
      421 gtgttggggt ttccagaaac attcaaagct ctttcagacc ctataagaag agaaatattg
      481 gttatgttga gggagaataa aatgtcagca ggagaaatta gtgaaaaatt taatatgacg
      541 aatgctacaa tttcttatca tctatctcaa ctaaaaaagg caggactcat atttgaaaca
      601 aagtataaga actttatttt ctacgaaata aatatttcag ttttcgaaga ggttatgctt
      661 tggttttcac aatttatgga gaagggagaa aaagataatg aaaaaagcga taaataaaca
      721 atttatttta tctactttaa tatgttttat accatttatt gtatcaatat atttttataa
      781 tagattacca aatgaagttg caattcattt tgataattat ggaaatccag ataattatgc
      841 tccaaaggtt atagcagctt ttggtgtacc attgttaatg ctatgtatcc atttgtatac
      901 atggtttaga cttgagaatg agcctaagaa agctaatttt tcaaatgtta tacaaaattt
      961 atcaaaatgg ggagttgcta tactttcagt aattatacaa ataatgatgg ttttatattc
     1021 aacaggagtt agtttggagt ctaattttta tacaggatta attttaggaa taatagttat
     1081 tttatttgga aactatcttc ctaaatgtaa acgaaattac tcagtaggaa taattttgcc
     1141 atggactctg aatgatgaag ataattggaa taagacacac catttagctg gatggatttg
     1201 gcttattggt ggaatattat taattattaa tgcatttatt aatattcctt tttataatat
     1261 ctttgtaatc tttgtaattg taatactacc atttatatat tcttatttga tgtataaaat
     1321 ttcaggtaaa aataactaat ataattaatc aatataataa tgtttggata agaataatat
     1381 atttcagaac tgagataggt aataaagaaa ttactaagat ttctatttta gttctgaaaa
     1441 aatcttactt atatgataac ttattgagat ttcaaaaaat aatataaatt aggctaatga
     1501 acataaaatg aaaatttgta tttaatattg aaaatataag tacagtgtga tattcttata
     1561 agaaaaacat aggaggtaga agatgaatac tagagaaatg aaagaatata gggtatttta
     1621 caataaaaaa ttaagcgaaa cagatgagtt ttttagagta tttggaagtc ttgatgataa
     1681 gacttattca gaaggggcta taagtaaaaa aaataaggaa ttaatgggac tagctatttc
     1741 aattttaagc cgatgtgatg agtgtatttg ttatcatatt gagggttgta ttaattctgg
     1801 agcaagtaaa gatgaaatta tagaagctat aaaaataggt gtaattggtg gcgggtctat
     1861 tacatatcca aacgcaagat ttgcaataaa aattttagaa gaatttaata tataattttg
     1921 atagacgtag tttatttaga ctaataataa tagtctattt aagtctttga aattaagctt
     1981 tattttctaa gttcacttct tattaagttt ttagaattgc atatataaat ataaattttt
     2041 aaataatata gtagttttaa attatttaca tcatagattg tattgagaat agtatattgt
     2101 cattattaaa tcttttatac attttttatt ttaaataaat taa
//
LOCUS       DAKBNN010000343         2101 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000343 DAKBNN010000000
VERSION     DAKBNN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.351"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(40..1236)
                     /locus_tag="OUB02_003810"
     CDS             complement(40..1236)
                     /locus_tag="OUB02_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCT8887523.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKYINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(1257..2078)
                     /locus_tag="OUB02_003811"
     CDS             complement(1257..2078)
                     /locus_tag="OUB02_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCT8887524.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT          656 a          411 c          221 g          813 t
ORIGIN      
        1 tacaattttg aataaatcat accatcattt atatttactt tatcttgaaa ccattaaatt
       61 tttcaatgct ttctcaagac cttttacagt caatggaaac atattaaatt cactttgtat
      121 cattctttga gtttgatacc aataattaat tgaatcccat ccatctctaa gttctggatt
      181 catccataca attttactgt actttctctt aaacctttta agccactcta taccaggtgt
      241 gtaattatat ggaccacgat agttaccacc aatatgaagc aactcagaag gcgacataga
      301 agcatctcca acaattataa ctttatagtt tttatctata ttttttaata tccattcagt
      361 attaatatat ttacttaacc aacattcagg tgttgtaaat aatctatcat aaatacagtt
      421 atggaaataa tatatcttta catctttaaa atgatttgat ttacttacag actgaaataa
      481 tgtattacat aaagtgctat accctctcat tgaaccacct gaatccataa gcaataaaag
      541 cttcactgta ttttttcttg gcttctcaaa aaataacttt aaatatcctg cattattaca
      601 agtctcttca atagtcttat ccaaatcaag ttctgtcttt ggagcatcta ttctagtaga
      661 aaattgacga agtctcttta gtgcaacttg aaattgacgc atatttaaaa cttcatcatc
      721 tctaaaatct ctgtattttc tttctcctgc aacttcaaga acagctctac cataagtaga
      781 atttccacct atacgtatac cagtttgccc ttttcctgaa tgtcctaact ctgaagtgcc
      841 tcctgtgcct atccaataat taccaccatt atgaggagag tcttgttctt ttattcgttc
      901 ctccaattta gctcttaatt tatcaagatt tatatttgga ttttgttcta atctttcaaa
      961 ctcttctaaa tctatatctg gttttttaag ccaattcata atctcttctg gaatttcttc
     1021 ttggtgttct atacctttaa aatactccaa aaacactgca tcaaacttat caaaatcact
     1081 ttcacttttt attaaaatca tcctgcaaag atagtaaaaa tttgaaaagt ttgaatagca
     1141 cagtccttta tccaatgctt caattaaagt catccattca tttaaagata catttaatcc
     1201 tcttgacctc aataaataaa aaaattcaat aaacataatt atccagctcc tattaactat
     1261 aagtgtcttt gcatagattc aatatcctca ttattcttaa gtagtattcc tgcaaaagga
     1321 acttcttttt ctattttttc aataggcatt ccactcaaag ttaatgcttg tatccagtct
     1381 attaactctg atgtactagg ctttttttgt atgtccttta aacttctaat ccaataaaaa
     1441 gtagtcatta cttgttccaa taggtgttct tctactttat caaaatgtac tttaactatt
     1501 tcttccatca tgtctctatc aggaaattct atataatgaa atatgcatct tcttaaaaaa
     1561 gcatctggaa gttctttttc cgcatttgaa gttataataa ctattggtct ttgtttagct
     1621 cttacagttt cttttgtctc atttatgtaa aactccattt tatccaattc ccaaagcaag
     1681 tcatttggaa attctaaatc cgccttatct atttcatcta ttaaaagaat tacttgttga
     1741 tttgagctaa aggcctctcc tagtttacca tattttatgt acttgctaat atcatctaca
     1801 ccttctcctc caaattggct atcataaagt ctttgaacag tatcatatac ataaagccct
     1861 tcttgtgctt ttgtagttga ttttatattc caaattacta aatctttttt taattcattt
     1921 gaaattgctt gtgctagcat agttttacct gttccaggtt cgcccttaat caaaagtggc
     1981 ttttcaagag caatagcaac attaactgat gccatcaatt ctggtgatac tacataatca
     2041 ctacttcctc taaagttaga tatctctttt tttatcataa atcctactcc ttttaaacag
     2101 t
//
LOCUS       DAKBNN010000344         2086 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000344 DAKBNN010000000
VERSION     DAKBNN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.352"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..886)
                     /locus_tag="OUB02_003812"
     CDS             complement(<1..886)
                     /locus_tag="OUB02_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HCT8887525.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYI"
     gene            complement(1718..1864)
                     /locus_tag="OUB02_003813"
     CDS             complement(1718..1864)
                     /locus_tag="OUB02_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887526.1"
                     /translation="MTAYIASLKEYYSFIETQIGDFIKKYGEDVVELCLHRVMILLCE
                     CGLA"
BASE COUNT          756 a          266 c          269 g          795 t
ORIGIN      
        1 taatataatg atttgcctct cttaaaacat ggtctgctaa aagtggtaat attatagatt
       61 ttatcttaca ctgttctatt cctgatgctc ctgcttcttt aaaatcttta aactcaacag
      121 tttcatttag agtttcttct gtaatgttct tgatattaga atcagtcatt tcgtttgttt
      181 tttcaattaa ttcattaaat tttatagcaa aatcatttga agtatttatt agttcacctt
      241 ctgaggggtc tagtaatcct ctcataaaca gagcatgttc catcataata tggtcccaga
      301 ataattctat ttctttagcg ttttttgact caatatctat tttattttca aggtcaacta
      361 cataagaacg atataaattt gcttcatgta ttatatgttc aagaagtaga gggtagtttg
      421 atgtaaatat agtacatgat agtacaccat ctaagactct ttctttaaaa ttaataagcc
      481 catcaagtaa tcttattgca tcagagttaa gttgagctac atagttcact aagtcttgac
      541 caacttgtgg gtttacacca ctttgtagat ttaattctct tgtagtgatg tttttgttta
      601 tttctatccc tgtaaactct tctgtttttt gttctgcaac tgatgtgaga gtagttacaa
      661 gctcttctga atataatata tcaggtctaa ttataccatt actagcacta acagtgtatg
      721 ataataaatc ttcaaattgc tttttatagt ggtcagcttc catagcaaga ttataatttt
      781 tatttgtgaa tcctgcttct aaaaaaagag catgctcttt cataattctt gaaaaaaata
      841 aatgtaattc tagtgataaa ataacatatt tttgattatc tatcataatt tcctcccttt
      901 agaaagtatc ctatttataa tactttattc gtaatattta aatttatgta tattataaat
      961 aaattaacta aattcaattg ctactaatat caacagtttt tattaagtta tgggcgaaaa
     1021 aattatctta ctgtgtttct tattagatat agatataatg atttaaggta cttgttggct
     1081 attatttatt aagagtacaa gtaatactag aaatttttat gagaattatg taatttataa
     1141 gtattatttt gatacaaaac aaatgttttt tgtaaaacaa gaacttttat agcaaaaaat
     1201 gaataaatat gaagcaagca taattttata ccacaacaat tattaattct aatagctgca
     1261 cttaatgttt taggtatggg atttaaaaag tataaaaaat taagtgataa atatattcca
     1321 ttatattgct tgtgcttgga gtagtatttt ctgtatagat gttaggattt aatccaactt
     1381 ctattttaca ggggattcta tgctggggtg tttcaattgg attaaattaa gtatgcaaac
     1441 aatttcaaga caatgataaa taattggatg tgtgatagga atatcaataa aaaacattat
     1501 taagaagaat tattttattt ataaaataaa acaagtaata agaataaata aggagcaact
     1561 aaaatgattt aaatatacta gaattaaaaa gagaatgtaa agttgtctag taatggatgt
     1621 aaaatatttt aaacgataag ctatgaaatt gatatattaa aatgggtaag aagaatatta
     1681 tataggattc ttcttaccca ttttaataca tttaatttta tgccaaacca cattcgcata
     1741 gtagaatcat cactctatgg agacataact ctacaacatc ttctccatat tttttaataa
     1801 aatcaccaat ttgagtttct atgaatgaat agtattcttt taaactagca atatatgcag
     1861 tcattatatg aacacattca tcaatcttat ctttaaagac aactaatccc cctaaaaaac
     1921 gaaaaataaa aatattcagt aaatagcaga tattatttca ctcataaatc tatatagtat
     1981 gatatattgc ttagatatat tttacaagtg gatatgtaaa actggttatt ataatcgcaa
     2041 aactggataa actttcattc ttaaaagaaa atttatccag tttaaa
//
LOCUS       DAKBNN010000345         2085 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000345 DAKBNN010000000
VERSION     DAKBNN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.353"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            604..1284
                     /locus_tag="OUB02_003814"
     CDS             604..1284
                     /locus_tag="OUB02_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887527.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
     gene            1732..2016
                     /locus_tag="OUB02_003815"
     CDS             1732..2016
                     /locus_tag="OUB02_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887528.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          918 a          132 c          299 g          736 t
ORIGIN      
        1 tccctttccc caaaatagga aatttttatt agcatttaat aatgattttt aatttttaat
       61 ataatattaa agaattattt aagaatattt aatctctaaa gcttaaatta caataatatt
      121 ataaaaataa aagtgatata ttgtgtaata atcgaaattt tatactttgg ttaaaataat
      181 tttatatata gtgcctaatg tataacctaa tgctcgtaga caataatgtg gatattatca
      241 aatttatttt tataatagaa attttaattt atatctatat taaattgtta aagatataat
      301 tatgagaata aaaaatatag ctttgaatta tataattgta tatgcttatc tttatacagt
      361 aaataaaaaa tcaaatgaaa taccaaataa agtgtatatc ttaaaataac ttactagaga
      421 tacaaggatg ctatgtgaat tttgaagtgt gcagtttaga taataaatat atataaataa
      481 taaagaaaat ggatttatag tatttaaaat ttcttataag ggtaaaaaat gctattagaa
      541 aatgtactaa aaatattttt ccaattcttt atagaaaaat aatgatattt aggagagatt
      601 gtaatgcaaa ttgatgtatt gataattgat ggagataaga atagttctca agagctgaaa
      661 aattttttag aaggaaaggg gataaatgtc tatttagcat ataattgtaa agatgctatt
      721 ataaaaatat ttagcaagaa atttgactta atatttttag agataatttt agttgatggt
      781 gatggttggg atttttgtaa aaaaattaga aatataacta cttgcccaat tattcatata
      841 acttatataa atgaaaatca aagtatttta aatgctttaa attctggagg agatgattat
      901 ttaataaagc ctttaaattt agaaatatta tatgcgaaag tgaaagctat tttaagaaga
      961 agagatttat ttttaaataa taataaaaat aatataaaac tagaagatta taatagagct
     1021 agtggagtaa taaagttaga aaataaaatg ataaagttaa ctccaacaga aaatagatta
     1081 ttaaattact ttatagagaa tgctgaaaag actctaacta ttaaggagat atatgaacat
     1141 gtatggatga atgagtattt agatgataat tatagtgtag tggttgctgt gagtggatta
     1201 agaaagaaaa tagagaaaga ttataaaaat cctcaaaaaa ttataacgat aagagaagta
     1261 gggtattact ttaacaagat ataaagcttt ataaacagaa tgaatgataa ggcatagata
     1321 atatgttagt atattacaag agtttttgtt ctaacgatat atttgtaaaa ttagatttaa
     1381 tttacttaat taaattatag cagtagaata aatataaaaa atgtctgaaa tattatagac
     1441 atcagtaaaa tatataaaac tataatataa gtcgtaataa taaaacaaat gaatattaat
     1501 taataaaaag atagtagcta tttttatata gctattattt ttttattgtt gtggatgaaa
     1561 atatacttat attttactaa tatacattta aaaattataa tttaaagcat taaaaaataa
     1621 atttgtattt tgttttatat aaagtaattt gaagaatatg gaaaaacttg attagtacat
     1681 tattaattag ggtataaaat ggtgtaaata gaatgataag gggtggatga aatgcttgta
     1741 tataataaaa gtttttatcc taatgacata tttccaagat tagatttttc aaaaataaaa
     1801 aaacagttaa aattgataga taatgacctg tcagattttg gaagcatatg tataatagaa
     1861 aaagaacatt atacaataag tgtaaacagt ataggtgaaa taaatgtgta ttatgattta
     1921 gagtacgaaa ataaggtgta tagaatagtt tatgagattg aaaagttatt taaatctcaa
     1981 gttggaaggt ttagcatatc tacatacaga aattgataat taaaaaagta gaaattaaaa
     2041 aacttaatac taaagatata gataaatata tgaggtaaca gctag
//
LOCUS       DAKBNN010000346         2084 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000346 DAKBNN010000000
VERSION     DAKBNN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.354"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            24..422
                     /locus_tag="OUB02_003816"
                     /pseudo
     CDS             24..422
                     /locus_tag="OUB02_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            410..1348
                     /locus_tag="OUB02_003817"
     CDS             410..1348
                     /locus_tag="OUB02_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCT8887529.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1732..1956)
                     /gene="secG"
                     /locus_tag="OUB02_003818"
     CDS             complement(1732..1956)
                     /gene="secG"
                     /locus_tag="OUB02_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCT8887530.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          762 a          274 c          259 g          789 t
ORIGIN      
        1 atataaaatc ttaaggagtt gccatgataa aaaaaataga gcatgtggtc attactaata
       61 ataatataga aaattatatc aatttttata aaaaatttgg tttttcatct aaagaaatga
      121 gcaaaaatta taaacttaat gctagaaatg ttaagataaa tcttaacata aaaggttata
      181 aatttttacc ttatgttaaa aattcaaaag caagtagaga taacttatgt tttaaaataa
      241 acacaaatat tgatgagcca aaaaaatact tagaacaaaa tgagattcaa attaagtgag
      301 gaattgtaga atgtacaggt gtctttggta atatgaaatc tatttatttg aaaaaaacta
      361 atggaaattt aattgaatta tcaaatcata gaatcacgga gagtaaacct tggaaaattt
      421 aatcctatca ataaatgttg ttcttccttt gtttctcaca atgtcacttg gatatgttct
      481 aaaaaaatta ggtctatttg atactcatac tttaaataaa atgaataatg tctgcttcaa
      541 gtctttcttg ccattattat tattttttaa tatatacaat acagatttag atggttctct
      601 taattttagg cttatggctg tagctgttat aattattttt tcaatcttct taatcttagt
      661 ctttattatt cctaaaattg aaaaagataa taagacaagg ggtgcattaa tacaaggtgt
      721 atttcgtagc aatttcgtta tatttggtat tcctgtgaca atttcactat ttggcgataa
      781 gagtattgga gtcacatctt tattaattgc agttattgta cctatgttta atgtattatc
      841 tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat ataaaacata tgattaaaag
      901 cgtcttaaca aatccactca taatcgcctc ttttatagga ttgacaatgc taattttaaa
      961 tattaatata cctacatttt tacacaaaac tattggtgat atttctaaaa tagctactcc
     1021 tttatcactt ataattctag gtggctcttt tagtttttct gctataaagg ggcatataaa
     1081 ccccattata ttgggcgtat gtggaaaact tatacttgta cctgctatat ttttaccaat
     1141 tagcattttt cttggattta gaaatgtaga attagcttca cttatgataa tgttatctgc
     1201 tccaactgca gtttcttcat ttacaatggc acaacagatg gattctgaca gtgaattagc
     1261 tggacaaatt gtagtattta cttctggatt ttctataatt acagtatttt taataatatt
     1321 tattttaaag cagaataatc ttatttaatg atatttgata acacaaattg gtattatgag
     1381 aaacatatat aaaatcgatt taattgcgta aaaacatatt attatactat ttttactata
     1441 taatataact ttgtgtatat tatctcatta aacatggtga aagtagtgtt gctatatatt
     1501 ttttagtttg aaaaaaatat ataaatacaa acaatatata aatacaaata ataaacgcca
     1561 acaaatagtc aatctataaa ataaaaactg gactaatcat ataaaatcat tagtccagct
     1621 tttattttac gtacttttta tgttgatgta cttttattaa aaactaaatt taatttttaa
     1681 atgtctagca aatgcactaa ttaaataatg gccagttatt caacaatatc actacttctt
     1741 aactataagt aatgccaatg catttataaa aaataatact gctgttatct ttgtaactct
     1801 agcaagaaat gcttgttttc cttttggttt aaagtaagct tgacttcctt ctccaccaaa
     1861 ttctgaaggt aaagcattct tagaatcttg aggcattatg ctaattacta gtaaaatccc
     1921 tagtactact tgcacaccca ttaaaatgtt gctcattctt gcccctccta tctgtttatt
     1981 acttaaattt cattactctt tagtgtatca caataatata gccatttcaa gactattatg
     2041 ctataaataa catacaaaaa aatatattgt tactataata tatt
//
LOCUS       DAKBNN010000347         2063 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000347 DAKBNN010000000
VERSION     DAKBNN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.355"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            345..1673
                     /locus_tag="OUB02_003819"
     CDS             345..1673
                     /locus_tag="OUB02_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCT8887531.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          730 a          222 c          353 g          758 t
ORIGIN      
        1 ctacagaaag aagccaaaat gtttttgaaa aaaatgaaat tttattgaaa aaaaatcaaa
       61 atgaatttaa cttaatagtt ataatctatt tttagtgtta caaatataca atgtatatta
      121 tacaatataa aaaatacatt tacttagaag tattattata tttggatttt tggtttttat
      181 aattttttta tatttaaaca tagctaaaaa taaaagatta cgaaagctaa gtagagttta
      241 ttttatttta ataaataata aagctatttt tctatatatt ggcataatat atgcttattt
      301 aactatgaaa ttaaaattaa ataggattaa tgaggaggaa aaaaatggga agtaatgaac
      361 tgaaaaagac tcttggcgta tcagcagcat tatcaactgt tgttggttcc gttataggag
      421 cgggcgtatt ttttaaacca caagcagtct atacattaac tggaggagct ccaggtcttg
      481 gtatattggc atggttaata gctggaatca taactataac agctggtctt acagctgcag
      541 aggtatcagt tgcgatacca aaaactggtg gaatgatggt atatataaaa gaaatttatg
      601 gagaaaaatt aggtttttta actggatgga tgcaaattgt attattttat ccagggatga
      661 tggcagcatt aggtgttata tttggagaac aagcatcagc actaattggt agtccatcac
      721 ttttacttcc aatagcaatt ggaattatag taatagttgc agggcttaat atgttaggtt
      781 caaaaactgg aggagtcata caaactgttt ctacaatttg taagttaata ccacttattt
      841 taataatggt tgttggattt attaaaggtg gtggaaataa tccaatactt acacctatgg
      901 ttggtgaagg attaagcctg ggtagtgtac taggtcaagt tttaattgct atattatttg
      961 cttttgatgg atggatgaat gttggtactt tagctggaga aatgaagaat cctggaaaag
     1021 accttccaaa agctataata ggtggattat cagttgttat ggctgtttat tttataataa
     1081 atttagcgta tctttgggta ttaccagcta atgaattagc aaattatgct tctcctgcat
     1141 cagcagttgc tgaagttata tttggttcaa tgggtggaaa aataatatct gtaggtatat
     1201 taatttcagt gtttggagca ttaaatggat ttttactaac tggttcaagg gtggcatata
     1261 ctttagctac agataagact ttaccaaagt atggtatatt ttctaaatta aattctgctc
     1321 aagttccagc aaatgcaata gcattagtat ctgttattgc atctatatat gctttgtcag
     1381 gtcaatttaa tttattaact gacttagctg tatttgctac ttggatattc tatgtattaa
     1441 cttttatagg ggttatgaaa cttagaaaga ctcatccaaa tataccaaga gagtataaag
     1501 ttccattata tcctattgtt cctataatag ctatagcaag tggtatattt gttgtagtta
     1561 atcaattgtg ttttgcaggt atgaaaacta ctatgatatc tataggaggt cttgtaataa
     1621 cagctatagg cttaccagtg tatgcttata tgactagagg agtaaaaaga taaaccaagt
     1681 agtttacaaa tgcttcataa ttaaaataaa agagactgtc tcaaatagag ataagtctct
     1741 tttttattgt taaaattgta aagatatgtt taattttaca aaaaaagagc gttccatgaa
     1801 ctaaaaagtt tattttaagg tattcatttt atttttagtt ttttgattat aaaattaaac
     1861 atctaaaaaa ttatttattt taaaatactt tattttaaag tgttttttat caaactaagt
     1921 ggtatctgta caacaaatat tatacatagc ttttatgaaa attaaatctt tctgttatat
     1981 ataatagaat attgtattat atagaaaata ttacctgatt aatatagtga tttataaatg
     2041 agattagaaa attttatttg ata
//
LOCUS       DAKBNN010000348         2055 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000348 DAKBNN010000000
VERSION     DAKBNN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.356"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(129..692)
                     /locus_tag="OUB02_003820"
     CDS             complement(129..692)
                     /locus_tag="OUB02_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCT8887532.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(790..1719)
                     /gene="glsA"
                     /locus_tag="OUB02_003821"
     CDS             complement(790..1719)
                     /gene="glsA"
                     /locus_tag="OUB02_003821"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCT8887533.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
BASE COUNT          688 a          395 c          194 g          778 t
ORIGIN      
        1 aagaaaacaa aaaaatagag attaaaaatc attgttttac ccataccaaa aactattttt
       61 tgattattaa gtaaattttg atgttcaatc tctaatatta tatctttaat taatccataa
      121 ttaattctct aactaggaca ttcctcctca agagtaggaa ctataaccat agctatatca
      181 tacttagaac cttcaacatt gtgttgtctt ttatttaaaa tttcatctat ctttgacttc
      241 atagtgtcac taagttcaac taattcatca tgagtaagag aaatatcatg ataatcaact
      301 atattttcat tttctaaaac atcttcctct gctgccttat aaaaaacttc actcatattt
      361 tcaaattttg atatgtacaa ttcttctttt tcttccccat tgtctaaaga aaaattaagt
      421 atcctattcc ctataaccac attttttgca cttggataat agtacttttc cacaatcccc
      481 gactttattt tttcctcaac taattctaaa ataccaactt tgtataacat ttttatatgg
      541 tagtttattt ttgcatgagg ttcttccaat aattgagata attgttttgc tgacaatggc
      601 gatttgtcaa atgcttttag aatatctatt ctacgtggat gcgcaatagc ctttatgcac
      661 tccaaatctc gtaataccaa tacctctttc attgacccct cctaaaaaat ttatttaatt
      721 catatttctt tacaatttaa ttatatatta aattatatgt tgttgtcaaa aaaatccttt
      781 cttaatattt taaaaaatat ttaatttgag tttatttgac aactcctcta aaactttaac
      841 tcctgcgacg ctgttacctt ttttgtcaag cgcaggtcca taaacaccaa tccccatgcg
      901 tcccggcaca gaagccatta taccaccccc aactccagat tttgcaggaa tgccaacttt
      961 aatagcaaat tccccagaag catcatacat tccacaagtc atcataaatg tcttgacaat
     1021 cctagaaacc atttcactca ttaaatgctc accattttct atatctactc cataatttgc
     1081 aagatttatt ccaattctcg ctaaatctac agaatctatc tcaattgagc attgtttaaa
     1141 ataaaggtct agaacatctt ctacattccc tctgataaat ccatcacttt tcaataagta
     1201 agccatagct ctgtttctat ctccagtcat tttttcagac ttatagacat catagtttat
     1261 tcctatatta tcattttttg ctaactttct aaagaaacta agcattcttt cttctttttc
     1321 ttctaaacaa ctcccattta taagggaagt ggttactata gcccctgcat ttatcattgg
     1381 attacagggt ttttttgtgt cattaatttc aagtttcatt attgaattaa atgggtcccc
     1441 acttggctcc attccaacgt ttgaaaaaac gtcttcccaa tcattatcca taagtgccat
     1501 agcaagcaca attggttttg aaatactttg tatagtaaac tttgtgctac agtttccagc
     1561 actataaaga ttattttcgc tatctatgat acaaattcct aaatcatttc ttctagcgtt
     1621 ttttaattct ggtatgtaac tagcgacttg tccataatta gtatacttct tattagaact
     1681 tataatttct tttaataatg tctcatctaa cgtattcata tgcacctctt atattatgtt
     1741 atatattcta ttttagcaca agtttatact ataataaaac aatgttaaaa aaaattatac
     1801 tttaaaatta ttttctacaa gaatcaattc atttttcaaa caatttaaat agtttaaaga
     1861 gcttaataat ttagtaattc aataatttaa caacctagca atttagtaat ctaataatcc
     1921 agtaatttaa caacctaata atttagcaat ctaataatct aactatctaa taatccagta
     1981 acttaacaac ctaataattt agtaattcaa caatctaaca attcaataat ttaacaacct
     2041 agtaatctaa ctatc
//
LOCUS       DAKBNN010000349         1998 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000349 DAKBNN010000000
VERSION     DAKBNN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.357"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            230..1633
                     /locus_tag="OUB02_003822"
     CDS             230..1633
                     /locus_tag="OUB02_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8887534.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHAIVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT          797 a          257 c          312 g          632 t
ORIGIN      
        1 ttactcaata tataatcaat acataattta taatttataa tttgtaatac tttattcaat
       61 acataagtag aaatctctat atacataaat tcattttttc atacatctta caaatttatt
      121 tttatttaga taacatgtat attttttcca tgaaaaataa ataataaaaa atagtaaata
      181 tggaaaatat aaaattgatt aacaataatt aattacctga ggtgtgaata tgaaaatgct
      241 aaacaacttt tttaagaaaa tcaacaatcc agctcctgtc tttgcacaga caaaaatgga
      301 aagagataat caaacagatg attcactttc tacaaaacca aagactacat ttagagacgt
      361 tgctggatta gacgaagtaa aagaagaact atttgaaata gtcgatttta tgaagtcacc
      421 acaaaaatat caaaaaatgg gtgcaaaaat acctaaagga gtattatttt atggtcctcc
      481 aggaacaggt aaaaccctac ttgcatctgc tgtagctggt gaaacaaact catcattttt
      541 taatgttact ggttcagagt tcgttgaaaa atatgtaggt gtgggagcaa aaagagttag
      601 aactctattt gaaaaagcta gaaaagaagc tccaagtata atatttatag acgaaataga
      661 tgctgtagga gcaaagagac atcttgaaag taacaacgaa aaagaccaaa cactaaacca
      721 acttttagtt gaaatggatg gatttaataa agattccaat gtattaataa taggtgctac
      781 caatagactt gacttattag atgaagcttt acttagacca ggaagatttg acagacacat
      841 tcatataggt gcaccaaact atcacactag atttgaaatt ctaaaggtac atacagatga
      901 caaacctata gacaaatctg taaatctaga acttttagct aaaaaaactc atggtttcaa
      961 tggggctcat ctttcaaata tagcaaatga agcagctata tttgcagtta gagatgacag
     1021 tgagtgtatt acatcagaac attttgacaa ggcacttgaa agagttattg cagggctaga
     1081 atctaaaaac tctgcattgg ttgaaaaaga gaaaaaaata gtagcatatc atgaagctgg
     1141 acatgctatt gtaagtgata tagtaggaat ttgtcctata caaaaaatat ctatagttcc
     1201 tagaggacaa gcattaggtt atgttcttca attacctgat gaagatagat atatttatac
     1261 taaggatgag ttgattggaa aaataaaaat attacttgct ggaaaggctt cagaagaatt
     1321 aatatttaat cataaatcta ctggtgcaaa ggacgactta aagaaagtca ctgaaatagc
     1381 taaccaaatg gtttgtgaat atggtatgag taacttagga tttatgacta ttgatggaaa
     1441 tgataagact ttcttatgtg ataaggttca aaaggaagca aatcgtatag ttgaaatttg
     1501 ttataaagag actttagaga tgttaaagga taatcttgaa gatttacatt ctgtttctaa
     1561 attcttattt gaaaaagaaa ctatgactca tgaagagtta aaagatttga taggaaaaga
     1621 agctgtaaac taatatagtt ttaaaaattt gaggctgtct caaaatagag ataagcctct
     1681 ttttattgtt aaagttataa aaatacattt gattttatac aaaaaagtgc ctcataaact
     1741 aaaaatttca ttttgagaca cttacatatt ttaattttgt ctatttacag aatattatat
     1801 ctacataaaa ctaaactgct gtaaacttaa ctacctccag taatgtttga agtggtataa
     1861 aagctagtag cctagtttct acacctataa tttatatttt acataactaa atttagtaga
     1921 tttttatact cacaatttag aacgattgaa aacttaaaaa tttcttatca gatatctaca
     1981 acttaattag gtatctac
//
LOCUS       DAKBNN010000350         1958 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000350 DAKBNN010000000
VERSION     DAKBNN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.358"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..788
                     /locus_tag="OUB02_003823"
     CDS             <1..788
                     /locus_tag="OUB02_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HCT8887535.1"
                     /translation="NFNSISIVYSTVLVSVWIAITIFYLVYTLLKYIKLKNMINDLSY
                     DVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNSCVILPPCDYTLKELNWIF
                     RHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDLDCEFYCDERVLKNCTIEE
                     KQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMFNEKYRNGILIAVILCLLS
                     SITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVYIDN"
     gene            1140..1709
                     /locus_tag="OUB02_003824"
     CDS             1140..1709
                     /locus_tag="OUB02_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCT8887536.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
BASE COUNT          775 a          166 c          320 g          697 t
ORIGIN      
        1 gcaattttaa tagtatttct attgtatata gtactgtttt agtctctgtt tggattgcta
       61 ttacgatatt ttatttagtt tatactttat tgaaatatat taagcttaaa aatatgataa
      121 atgatttgtc ttatgatgtt gaggatgata agataaaaaa tctttatgaa aatttattaa
      181 aagagcttaa gataaataaa aaaatacggc ttaaatattc atatgaagtt gaaagtccag
      241 tattttttaa ttcgtgtgtc attttaccac catgtgatta tacattgaaa gagttaaact
      301 ggatatttag gcatgagttg atgcatttta agagtaaaga cctttatatt aagtatttgg
      361 tgatattttt aaaatgtgtt tattggttta atccttttgt gtatataatg gagaagttta
      421 tagatttgga ttgtgaattt tattgtgatg aaagagtttt gaagaattgt acgatagaag
      481 agaagcagga ttatgcattg acaataatta gtgcaatgag aaaaggttca aatagttcaa
      541 ataaatttgt ggctggatta cataaggagt cagatattaa aaaaagagtg tttagtatgt
      601 ttaatgaaaa gtacagaaat ggtattttga tagcagttat tttatgttta ttatcttcta
      661 ttacatattt taaaattgat ttaattacta ctaaatcaat attttatcca ctttcaaaac
      721 aaacacctat tgaaaacaga actaattcaa aacctacata tcagattaat gtttatatag
      781 ataattaaaa aatacaaagt ttaacaagag attaatataa ataattttaa taagtatctt
      841 tcacatatgt taaatttact ttaattatat taaagccaat gaattattct ttagaagctc
      901 caatgccagt aaatacaggt aaagaaaaaa ttactacaat tgtagattaa aaatgttcta
      961 atatttaaaa aaatgcaaaa gtgtaagaag ggtaaaaatc tattataata cattaaaaac
     1021 taaatttaga aaaatttaca taattagaaa aatgatttga taaaaaataa agttaaatat
     1081 taactaagta aatttgaaaa agaaaaaata taaagtacaa atttaaaagg aggtaactaa
     1141 tgaaaaagaa gttggaagat atgactttag aggagttatg gaacttgttt cctatatttt
     1201 tgactactca caaagaatgt tggaaaaatt ggtttaaaga agaacaatat agactaactg
     1261 gaatattgcc aatgcaaaaa attgttagaa taagtcatat tggaagtact tctgtgagta
     1321 cgatttatgc caagcccatt gttgacatac tgattgagat aattcctgat tataatatga
     1381 aagatattaa agatttgatt gagtcgaatg gatatatttg tatgtctgag agcagtagta
     1441 ggctttcact taataaaggg tatacagtag atggatttga agaacgagtt tttcatttgc
     1501 atataaggta tgtaggtgat aatgatgagt tgttttttag agattactta attgaccacc
     1561 cagatgttgc aaaggagtat gaaaaactta agttggacct ttggaagaag tatgagcata
     1621 atcgtgatgc ttatacagat agtaaactag attttgttag ggagtttact gaaaaggcaa
     1681 aggtgttgta ttctaataga tataattaga aataggtaca attagatatt tatcttatat
     1741 attgtattat attttttaag aaagtaatta atagaaaaag ggcttgtttc aaaatggaga
     1801 gataaatccc tttttcattg ttgaaaggat aaaaatatat ttgatttcat gtaaaaaaga
     1861 gtgcctcatc aactaaaaag tttattttga gacacacaca tttttactaa ggtaaataaa
     1921 atatcacatt tgattaaatt ttaagttaat gaatatat
//
LOCUS       DAKBNN010000351         1948 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000351 DAKBNN010000000
VERSION     DAKBNN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.359"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            164..1741
                     /locus_tag="OUB02_003825"
     CDS             164..1741
                     /locus_tag="OUB02_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HCT8887537.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT          802 a          186 c          368 g          592 t
ORIGIN      
        1 tatattttta tcatttaaat aatgaaaaat aggcttgtct atatttttag atagttttct
       61 tattattgat gaaaaatgta ataattttaa actgtaaaat agtgtaaagt tattttataa
      121 tataaatata gtaagatttt aaacaaaata agggggaata ataatgaaag caccaaaaac
      181 tattttaaca atactgacaa tagcgcttac tttaagtagc atttctataa taccatcata
      241 tgcacttaca gaggaaaaat taataggtaa tggtagatat gaaactgctg taaagataag
      301 tcaaaaggca tacagttcta gttctaatgt agtgttagtt aatgacaatt cattagctga
      361 tgcactttca gccacaccat ttgcaaaggc taaaggagca ccaatacttt tgacagaaag
      421 tgataagcta gatgatagga ctgaaaaaga gataaaacgt ctaggagcta aagatattta
      481 tttgatagga ggtactgctg tacttaataa agatatagag aacaagttaa aaggtaatgg
      541 acttaatgta gaaagaatta atggtaagga tagatatgaa acttcactga ttttggctaa
      601 taagcttaag gatataaaag atattaagga agttgctgta gtaaatggtg aaaaaggatt
      661 aagtgatgct gttagcgttg gagcaccagc agctcaaaat aagatgccta taattttgtc
      721 tagtcctaag gacggagtag aagcatttga taaatttata agggatgaga aagttataaa
      781 agcatatgta attggaggta cgacttcagt ttcaagagct gtagaaaaga gtcttcctaa
      841 tgcagaaaga gtgagtggga aagatagaaa tgagactaat gctaaggtca tagaaaagtt
      901 ttacactgat acaaatttaa gtaatttata tgtaactaag gatggtagta aaaatgagaa
      961 tcaactaata gactcgttag ctgttggagt attggctgca aaaaatgagt ctcctgttgt
     1021 tttggttggt aataaattaa acacaaaaca aagggacata cttagtacta aaaaattaaa
     1081 tactataaca caggtaggtg gaaatggaaa tgaagaagct tttgatgaaa ttaaaagtct
     1141 acaagaaaaa actgtgtttg aagcaaaaac tgttgaggaa ttaactgata tgataaatat
     1201 agcaagtcct aatgatatta taaactttaa accaaaagag aatactgtaa atgaagcttt
     1261 tagaatggta acaaataaac caataactgt gaatataaag ggtgattgtt caaagactct
     1321 tacagtagat atgcctaatg gtgaagtgaa taactatgct actttggtaa atgttatagt
     1381 tagaaatatt ggagaaggtg gatttaataa ccatgataca atcactatat tgtcagttcg
     1441 tgacaaaaat ggtagagtta tagaaaatac tagaaattca gatatagata cgttgatgat
     1501 attagcttct gctaatgata caaaattgat taatgatgga tatataggaa aacttataga
     1561 taattcatct aatagtgata taactaataa tggtactata gataaaaaag ttaatcaggt
     1621 tgaagattta gaggctaaag tagattcaat tgaaaaagct atagattcta taagtcaaaa
     1681 agtcaataaa atacaagata tactagataa gcttggattt ctaaagaaat ttcttagcta
     1741 agaatatata tatttaagtg aagtgtgtgt tgtaattgtt ctaaatttat gtagttaaac
     1801 aggtttatat acaaatagat ttatagataa atatgtagtt tagtatatat ttgaataagc
     1861 tttggggagg taacatgggg attggaattg aatgtaacaa aaaaataaat aaaagtattg
     1921 acaaaactag aataaagaaa aataatct
//
LOCUS       DAKBNN010000352         1913 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000352 DAKBNN010000000
VERSION     DAKBNN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.360"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            39..1733
                     /locus_tag="OUB02_003826"
     CDS             39..1733
                     /locus_tag="OUB02_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCT8887538.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKIYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
BASE COUNT          792 a          161 c          334 g          626 t
ORIGIN      
        1 taaatacaat aaagtaaagt tgtaaaggag tgctttaaat gaagttttca gagtttaagt
       61 atgaaagacc caattataat agtatgaaaa aagagttttt gagttgtgta gaagatataa
      121 ataattctcg taattataaa gagcaacaaa aaaatataca taagattaat ttattaagaa
      181 ataaaataga aacgttgtca aatatagctt cgataagata tagtacagac acatttaata
      241 aattctacaa agaagaaaaa aattattggg atgaatatat gcctttgtat gaagaattaa
      301 attcatattt ttataatgcc attgtaaatt caaaatttaa atatgatttg ataaaagagt
      361 ttggagagca atttttcaca atagtagagt attctttaaa aagtttttcc aaagaaataa
      421 taagtgaatt gcaggaagaa aataaattgt gttcagagta tactagactt ttagcttcag
      481 ctgagataat gtttgatggg aaaataagaa atttatctgg tatggggaaa ttcatgtatt
      541 ctaagaatag aaaaacaaga gaattagcaa acaaagcata ttataatttc tttgaagaaa
      601 atgaaactaa atttgatgac atatttgata aattggttaa acttagagat aagatgtcta
      661 aaaaattagg ttttgaagat tttgtagagc ttgggtatgt tagaatgatg agaagtgact
      721 acagagaata tatgataaaa aatgttagaa aacaagtact aaaatatgtt gttccaatgg
      781 caaatgagct ttatgaaaaa caagctaaaa gaattggtct agaatattta agttacatag
      841 atgaaggcgt ggaattttta acaggaaatg caagtttaaa gggagattca agatacataa
      901 ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga aacaaatgaa ttttttgatt
      961 ttatgttaga aaatgaactt atggatttag aaacgaagaa aggaaaagga gctggagggt
     1021 attgtactta tataccagat tataaatcac cattcatttt ttctaatttt aatcaaactg
     1081 ctgatgatat agatgtgtta acacatgagg caggacatgc atttcagttg tatatgtcta
     1141 gatggattga tatgccagaa attaattttc caacgcttga tagttgtgaa atccattcaa
     1201 tgagtatgga atttataaca tggccgtgga tggatttatt ttttaaagaa gatacagata
     1261 agtacaaatt cactcactta tcatcttcaa ttaaatttat accttatgga gtaattgttg
     1321 atgaatttca acactatatt tacaaaaatc ctaatgtaga taagtcaaaa cgaaaagaaa
     1381 tctggagatt tttagagaaa atatacttac ctcatagaaa atatggtgat aattcttttt
     1441 tagagagagg ttgttggtgg tttaaacaag gacatatatt taaaaatccg ttttattata
     1501 tagattatgt attagctcaa atttgtgcgt tacaattttg gaaaaaaatg attcaagata
     1561 gagatgcagg atggaaagat tatataaata tctgtaaagt tggaggaaca aaatcatttt
     1621 tagatattgt gagtatggga aatttgtatt ctccatttga tgatggctgt attgagtcta
     1681 ttataggtga tgttaaatct tggtttgatg aaataaatga cagcaaatta taaaaaatta
     1741 tgagaatttt attgtataaa agtgaaaaat taaaaaatat ataaaaagtg attatttgtt
     1801 gtgaaaatag tcttgttttt aactaaattg ataaaataaa ctaaaaaaga tttgtactta
     1861 aaaaaaaaag aaaatacaag aaatatgcaa tatcaacatt tggaaaaaaa agt
//
LOCUS       DAKBNN010000353         1898 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000353 DAKBNN010000000
VERSION     DAKBNN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.361"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            88..1137
                     /locus_tag="OUB02_003827"
     CDS             88..1137
                     /locus_tag="OUB02_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCT8887539.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKVGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1286..1633)
                     /locus_tag="OUB02_003828"
     CDS             complement(1286..1633)
                     /locus_tag="OUB02_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCT8887540.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          715 a          251 c          281 g          651 t
ORIGIN      
        1 attataagca tagatacatt tttgtaacaa ctacaataaa tttttaatac tatatactat
       61 tcgtatgtct tatgggaggt atttattatg tttaaaaaaa tagtcgcaat agcaagctct
      121 atcattgtaa gtacaatatg tctaactgga tgtgatttta actctaatga caaaaaaggt
      181 gaatcttctt cgtctaaaaa tctggataaa gtaactatag cagaagttac tcactctgtt
      241 ttttatgctc cacagtatgc tgctattacg aaaggattct ttgaagatga aggtataaag
      301 attgacctta taaataccca aggtgcagat aaaactatgg cagctttgat ttcaggtgaa
      361 gcaaacgtag gtttaatggg tcctgaagca tctatatatg tatataatca aaacaaaaac
      421 gattatgcag tgaactttgc tcaattgaca aaaagagatg gtagttttct agttgctagg
      481 aaaaaaatgc ctaatttctc ttacaatgat ttaaaaggaa ccgaaatatt aggtggtaga
      541 aaaggtggag ttccactaat gacttttgaa tatgttctaa aagaaaaagg tcttactatt
      601 ggagaaaatc taaaagtagg caatgttaat gtaagaactg atgtacaatt tggtgtaatg
      661 gctggagctt ttgcaggtgg agagggtgac tttacaacag catttgaacc tacagcaaca
      721 gcaatggaaa aagaaggtag tggatatata gttacatcta taggaaaaga ttctggagaa
      781 atacctttta cagcatactc tactacgaga tcatacatga aaaacaataa agatttaatt
      841 caaagattta caaatgctgt ttataaaggt caattatgga ttcaacaagc atctagcaaa
      901 gaagtagcta aggctatgca acctttcttt gaagatatat ctttagaaga tttaacaaca
      961 gttgtagata gatataaatc aatagatgca tggtctcaaa atcctgtatt aaaagaggaa
     1021 ggtttgaaga aattgatgac tgttatgaaa gaagctaaag aactagataa ggaagcacct
     1081 tataaagata tcgtaaatac tgagtttgca aacaatgcta taaaaaatat taaataaaat
     1141 atattagttt tagctagata ttttcttttt ttataatgaa aattaacaaa aatataaaaa
     1201 gagcctttta aaagactctt tttatatttg agattaaagt ttatataata aaatagatat
     1261 taaagtacta actgtactta tatatctatc ctctgtattc tgctccagca ttaatttttg
     1321 taatttgaac ggctaccatt tcttcattgc ttccattagt aatttcatgc cactctcctg
     1381 ttggtacaaa tacagtatct ccgtttttta catcacattc atgctcacca agtttcattt
     1441 taccttctcc ttttaggaac acaaatattt gttcaccatt agggtgtcta tgagcattaa
     1501 gaacttgatt aggtttaaaa taataaactt ctgaagttat atcgttacca gcaaatactg
     1561 gtatttttgg aactgctgta tcattatatt gagctaagct tccgtctaat agattctttt
     1621 tattttcatg catactagaa atacctcttt tctatattag atttactatt attataccaa
     1681 tataagaaaa tagtattctc actttacaca ataggaataa aattctttta tttttaaata
     1741 taagtataat actattttta tagttagaaa aatacaatcc ttataagata taaaaaatta
     1801 acataatata ataaattcta aaagtatttt tagatatcta aactttaata taaataacat
     1861 atcaatattt tttataaaaa attaaaatta tttaatac
//
LOCUS       DAKBNN010000354         1891 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000354 DAKBNN010000000
VERSION     DAKBNN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.362"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            85..201
                     /gene="rrf"
                     /locus_tag="OUB02_003829"
     rRNA            85..201
                     /gene="rrf"
                     /locus_tag="OUB02_003829"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            208..282
                     /locus_tag="OUB02_003830"
     tRNA            208..282
                     /locus_tag="OUB02_003830"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asn,seq:gtt)
     gene            287..361
                     /locus_tag="OUB02_003831"
     tRNA            287..361
                     /locus_tag="OUB02_003831"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Glu,seq:ttc)
     gene            367..442
                     /locus_tag="OUB02_003832"
     tRNA            367..442
                     /locus_tag="OUB02_003832"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Val,seq:tac)
     gene            448..524
                     /locus_tag="OUB02_003833"
     tRNA            448..524
                     /locus_tag="OUB02_003833"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Asp,seq:gtc)
     gene            535..609
                     /locus_tag="OUB02_003834"
     tRNA            535..609
                     /locus_tag="OUB02_003834"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Thr,seq:tgt)
     gene            624..708
                     /locus_tag="OUB02_003835"
     tRNA            624..708
                     /locus_tag="OUB02_003835"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:658..660,aa:Tyr,seq:gta)
     gene            718..791
                     /locus_tag="OUB02_003836"
     tRNA            718..791
                     /locus_tag="OUB02_003836"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:750..752,aa:Gly,seq:tcc)
     gene            802..878
                     /locus_tag="OUB02_003837"
     tRNA            802..878
                     /locus_tag="OUB02_003837"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:836..838,aa:Arg,seq:tct)
     gene            888..963
                     /locus_tag="OUB02_003838"
     tRNA            888..963
                     /locus_tag="OUB02_003838"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Gln,seq:ttg)
     gene            975..1050
                     /locus_tag="OUB02_003839"
     tRNA            975..1050
                     /locus_tag="OUB02_003839"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1008..1010,aa:Lys,seq:ttt)
     gene            1053..1141
                     /locus_tag="OUB02_003840"
     tRNA            1053..1141
                     /locus_tag="OUB02_003840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1087..1089,aa:Ser,seq:tga)
     gene            1159..1249
                     /locus_tag="OUB02_003841"
     tRNA            1159..1249
                     /locus_tag="OUB02_003841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ser,seq:gct)
     gene            1258..1334
                     /locus_tag="OUB02_003842"
     tRNA            1258..1334
                     /locus_tag="OUB02_003842"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1292..1294,aa:Pro,seq:tgg)
     gene            1420..1495
                     /locus_tag="OUB02_003843"
     tRNA            1420..1495
                     /locus_tag="OUB02_003843"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Trp,seq:cca)
     gene            1556..1632
                     /locus_tag="OUB02_003844"
     tRNA            1556..1632
                     /locus_tag="OUB02_003844"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Pro,seq:tgg)
     gene            1639..1715
                     /locus_tag="OUB02_003845"
     tRNA            1639..1715
                     /locus_tag="OUB02_003845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1673..1675,aa:Ile,seq:gat)
     gene            1719..1794
                     /locus_tag="OUB02_003846"
     tRNA            1719..1794
                     /locus_tag="OUB02_003846"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Phe,seq:gaa)
     gene            1802..1878
                     /locus_tag="OUB02_003847"
     tRNA            1802..1878
                     /locus_tag="OUB02_003847"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1836..1838,aa:Met,seq:cat)
BASE COUNT          475 a          440 c          516 g          460 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaaatgtggt tataatagcg gagaagatac acctgtttcc
      121 attccgaaca cagaagttaa gttctctagc gctgatggta cttggtggga aactacctgg
      181 gagagtagga catagccacg tgaatgttcc aaggtagctc aacggtggag caaccggctg
      241 ttaaccggta ggctgtgggt tcgagcccca cccttggagc cattatggct cgttggtcaa
      301 gaggttaaga caccgccctt tcacggcggt aacaggggtt caattcccct acgagtcacc
      361 atttgtggga tcatagctca gctgggagag cacctgcctt acaagcaggg ggtcacaggt
      421 tcgagccctg ttggtcccac catttgcggc ctggtagttc agttggttag aatgccagcc
      481 tgtcacgctg gaggtcgagg gttcgagccc cttccaggtc gccaaaataa aaatgctggt
      541 gtggctcaac ggtagagcag ctgacttgta atcagcaggt tgtaggttcg attcctatca
      601 ccagctccat gtaaaactaa tacggaggat ttcccgagcg gccaaagggg gcagactgta
      661 aatctgttgt catcgacttc ggtggttcga atccaccatc ctccaccaat taaatatgcg
      721 ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt cgatcccctt
      781 cacccgctcc aaagaaagcg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      841 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatatattgg ggattagcca
      901 agtcggtaag gcacatgact ttgactcatg tatgcgtagg ttcgagtcct gcatccccag
      961 ccaaataaaa atatggtcta ttagctcagt cggtagagca cctgactttt aatcagggtg
     1021 tcccgcgttc gagtcgcgga tagatcacca atggagaggt gtccgagtgg tttaaggagc
     1081 tggtcttgaa aaccagtgac ttcgaaaggg gccgtgggtt cgaatcccac cctctccgcc
     1141 aaaaaaatga ctgaatttgg agaagtactc aagtggctca agaggatccc ctgctaaggg
     1201 attaggctgg gtaaccggtg cgagggttcg aatccctcct tctccgccat tcaaaaacga
     1261 ggtgtagcgc agtttggtag cgcacatggt ttgggaccat ggggccgggg gttcgagtcc
     1321 cttcacctcg accaacttaa gcttttagtt cagttggtta gagtgcccaa ctcatggcta
     1381 gtaattctag ggtttgagtc catgaaggtt attcttcata ggggtgtagc tcagttggta
     1441 gagcgtcggt ctccaaaacc gtgcgccggg ggttcgagtc cctccacccc tgccaaataa
     1501 aaaggacttg aaaaaagttg gattttagag tagtataaga tagtaaataa gatttcgagg
     1561 tgtagcgcag tttggtagcg cacatggttt gggaccatgg ggccgggggt tcgagtccct
     1621 tcacctcgac caagaaaagg gcctatagct caggtggtta gagcgcacgc ctgataagcg
     1681 tgaggtcgct ggttcgagtc cagctaggcc caccatttgc ccagatagct cagtcggtag
     1741 agcaggggac tgaaaatccc cgtgtcggtg gttcgattcc gcctctgggc accaggaata
     1801 cggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
     1861 ctcctattac cgctaccatc taactatatc a
//
LOCUS       DAKBNN010000355         1849 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000355 DAKBNN010000000
VERSION     DAKBNN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.363"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            11..280
                     /locus_tag="OUB02_003848"
     CDS             11..280
                     /locus_tag="OUB02_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCT8887541.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            306..1661
                     /locus_tag="OUB02_003849"
     CDS             306..1661
                     /locus_tag="OUB02_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HCT8887542.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDSVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT          677 a          199 c          407 g          566 t
ORIGIN      
        1 ggatattatt atgaaagcaa ttttaacagt aattggaaag gataaaccag gtatagtggc
       61 aggaattagt gatgaattat acaagcaaaa tatcaacata gtagatatat cacaaaaaat
      121 attacaggat tattttacta tgattatgat agttgattta gagaaatcat taaattcttt
      181 tgagaaaacg gttgaggact tggttgaaag aggtaagaaa ttatcagtgg atgtaaaaat
      241 tcagcgagaa gaaatattta attcaatgca taatctgtag tagacagtaa ggggaggtga
      301 taatagtgaa tgtaggaaat atacttgaaa caataaagat gatagatgaa gaacagttgg
      361 atataagaac tataactatg gggatttctt tattagattg tatagaccct gatggagata
      421 aagctagaga aaaaatatac aataagatta tgagttctgc taaagatttg gtgaaggttg
      481 gaaaagatat agagcgtgaa tttggtatcc caattgtaaa taaaagggta tcagtaactc
      541 caatttccat tattgcaggt gcaacagatg aaaatgacta tgtaaagttt gcacagacat
      601 tggataaggc tgcagaggat ttaggtatag attttattgg tggattctca gcattagttc
      661 aaaagggata tacaaaaggt gataagatac taattaagtc aattccaaaa gctctagcaa
      721 gtactaataa agtatgtgct tcagtaaatg taggttctac aagatgtggt ataaatatgg
      781 atgcagtgaa agaaatggga gaaatcataa aagaaactgc agaattaaca aaagatgcaa
      841 aaggttttgg ttgtgcaaaa ttagtagtat tttgtaatgc agtagaagat aatccattta
      901 tggcaggagc tttccatggt gttggtgagg ctgatagaat tataaatgtt ggtgtaagtg
      961 gccctggtgt tgttaagaga gctttagaga aagtaaaggg agagcctttt gatgttgttt
     1021 cagaaacagt taaaaagact gcatttaaaa ttacaagagt tggacaactt gtggcaaagg
     1081 aagcttctag tagattggat gttccatttg ggataataga cttatcacta gctcctacac
     1141 ctgctattgg tgattcagta gcaaatatat tagaggaaat gggtcttgaa gttgttggta
     1201 ctcatgggac tacagcagca ttagcacttt taaatgatgc tgttaaaaaa ggtggagtaa
     1261 tggcatgttc acatgttgga ggacttagtg gagcgtttat accagtatct gaagatgcag
     1321 gtatgattga ttctgttatt aaaggtgctt taagtataga taagcttgaa gcaatgactg
     1381 ctatttgttc tgtaggtctt gatatgatag ctgtgccagg aaatactaca gcaggtacgc
     1441 ttggagccat gatagctgat gaggcagcaa taggtatgat taataataag actacagccg
     1501 ttagaattat accagcccca ggatgtgatg taggtgatat ggtagaattt ggaggacttt
     1561 taggacgagc accagtaatg cctataaata caaattcatc tgaattattt actcaaagag
     1621 gaggaaggat tccagctcct atacattcat ttaaaaatta gttatattac atataataat
     1681 atttaattaa atatttagat ggttaaatta aagaagtcta tgcatttaaa tattatataa
     1741 gataatgaac tttgttaaga taataaactt taaaaattag ttgttaagat tatggcaaaa
     1801 ataaagcgat acatggacat ttaaaaattc atgtatcgct tttatttta
//
LOCUS       DAKBNN010000356         1849 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000356 DAKBNN010000000
VERSION     DAKBNN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.364"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            169..1509
                     /locus_tag="OUB02_003850"
     CDS             169..1509
                     /locus_tag="OUB02_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT8887543.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVRFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          731 a          127 c          296 g          695 t
ORIGIN      
        1 gtttaattca atttagcaaa tttagtgata taattaaaaa agaaaagaaa tatggactta
       61 tttggaataa ttttgacaat ctatttacta cacgtgtaga gtcgtatata attaaattat
      121 acataaaata taaaaaaata atttagtaat acgttaggag aaagtattat gataaagtca
      181 agcttatttt ggaatatttt cattgcgata tcctttatga tagaatggtt tctttgtaag
      241 aaaatgctag attatacaag tgagaaaagg atttctaaca aaaaaataaa cttttgtatg
      301 gtaggtatta ttgtttttgc tgacataata tatttttttg atgtacctcc aaatataaga
      361 gtaattatat ctatgataat tactattgca ttttataaaa tctcatatga tgtgaggttt
      421 ataaagtctg tattaatatc cttaatttat tggatgttac taatcgccac agatgctttg
      481 agtatatctt taacagtttg gcttaattct ataaatgaca tgagtagtat ggggacggga
      541 gatatataca gaattgagtc tataatattg ggaaagatac ttttgattgt gtctttgctt
      601 ttatatagag ccttaaagat aaaacttaat atagaaataa ataaaaggac attattttat
      661 atagttattc ctatagtcgc taatatagca tctttttttg taatttttga atacatattt
      721 aatccagggt ctggtagctt aatacataat ttccaattcg ttatgatttc tgttgtattg
      781 cttatatcaa gtatcacttt agttttaatt gctaaaaaat tacagcaata taatacttta
      841 gttgtagaaa acaatatgat taaaaatact ttgaagtatg aaaatgagta ttatgaagat
      901 atagaaaaga aatatttaaa aactagatta ctaagtcatg atatgaaaaa tcacatattg
      961 tgtataaatg ctatgattaa aaaaggtatt gatgttactt cttatttaaa taatttgcag
     1021 gtagaaattc agagtaatga cttgttattt aatactggta attttatatt agatgctata
     1081 ttaagtgaaa agaaagaggt ttgtgataag aatgatatag aactaaaaat aggaataaat
     1141 tttagttgtt gtgaagctgt tgaattagtg gatatatgtc atatattttc aaatatttta
     1201 gacaatgcaa ttgaggcatg taataaaatt gatagtggaa atagagttat ttgtgttaaa
     1261 ggtgatgtta tgcaaaattt ttatttaata agagttgaaa atactaagat gaataagatt
     1321 attactaaaa atggagatat tttgacagac aaaaaaggtt ctcttcatgg acttggaata
     1381 aaaagtgtaa aaaactctgt aaataagtat ggtggtaatg ttgtaattga atatgatgat
     1441 aaaaaatttg tagttaaaat tgctattcca cttagaaatg tgaagaaagc agttaatcat
     1501 gtgatttaaa tgtatataaa tttgatgtgt agaaatttaa tatataagaa ttaatgtata
     1561 aaagtgatgc atagaagttt aatatataag aattaatgta taaaagtgat gtatagaagt
     1621 ttaatatata ggaatttaat aagtataaaa gtgatgcata gaaatttaat atataagaat
     1681 ttaatatgta taaatgtggt gcatagaagt ttaatgtaaa aatttggtgt ataagaattt
     1741 aatatatata tttattttaa tatatatatt gaagatatat aattaaatca tataatgaca
     1801 tttgtatatt agaattgttt attgagtatt agaaactttt aaggttaga
//
LOCUS       DAKBNN010000357         1832 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000357 DAKBNN010000000
VERSION     DAKBNN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.365"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            284..1642
                     /locus_tag="OUB02_003851"
     CDS             284..1642
                     /locus_tag="OUB02_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCT8887544.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDLKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRVL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
BASE COUNT          675 a          184 c          336 g          637 t
ORIGIN      
        1 gatagtgata caatattaac gaaatatagc tacttttaat aataacggaa aatatttata
       61 ttaaattaat aaatatttta aatgtggtat ccataaaaaa taataaacat aagataatta
      121 taaagatata ctgtgttcaa atgatattaa acaagattta tattatttga aagcagtata
      181 ttataatgcg aacaatacaa ttctaaactt tgcattgtca atttacagtt cgataaacta
      241 attaaaaatt aaatttatat aaagagggaa aagaggaata gggatgttta taaggaaaaa
      301 tgttataaag ttagcaattc ctataatgat agagcagacg tttgtcatgc ttttaggagt
      361 atttaataca atgatggcag gtcatattgg ggaagaagct gtatctgcag ttggaatggt
      421 tgattcaata aacaatatat ttatatctgt atttgctgca ctatctgttg gagctactgt
      481 tgttgtagct caacaaatag gaaaaaaaga tggtgaaaag gtgaatgaaa ctattaagca
      541 ggctctagtg tctggagtaa tattatcctt tacaataaca atattgatgt gggtttttag
      601 aactggtgta attaatttat tttatggttc cgcagaagaa ttggtaaaat caaatgcaaa
      661 attatacata gagtttacat tatttacgta tccatttatt gcgacacaac aaatagcaaa
      721 tggtatttta agagggtgtg gagatttaaa gaaacctatg tatgtgacta tgtttatgaa
      781 tatagtaaat gtaacattag gatatatact tatatatggt attgatttaa atcctctagg
      841 aataaatttg caaacaccat cctatggcat aacaggtgca gctatatcta ttgctatagc
      901 tagaataatt ggatgtatag taattataag agttttattt aaaggaaacg aatttatatc
      961 tatgggtaaa atatttccat ttaagattga tgttgaaact caaaagtcta tatttaatat
     1021 aggaatacca gctggagttg aacaactact ttttaatgca ggtaaattga ttgttcaagt
     1081 atttatagtt actatgggaa catcagcaat tgcatccaat gcaataggaa tgtcaattgc
     1141 atccataata aatgtaattg ggaatgcttt agctttgtct gctacaacct tggtgggaca
     1201 gtatatagga cgtggagata ttaaaggagc taagagtact ttattatatt taactaaatt
     1261 tgcaacaatt tgtttattta tagtaggagc tatatttata ccaattgcac cttggatttc
     1321 aagtatgtat actcaaaatg ctagtgtaat tgacatctca acacaattaa ttcgaagtga
     1381 ttgtttagct atggtaatat ggcctatatc ctttgtatta tcagcagggc taaagggagc
     1441 cggtgatact agatatacaa tgatgactgc agtaataggt atgtggattt ttagaatttt
     1501 tacaggttac gtattggggg ttgtattaga atttggagtg ttaggaattt ggataggtat
     1561 gtatactgat tggcttgtta gagggacttt atattgttta agattaagag gaacaaagtg
     1621 gctaaaacac aaagttgcat agaatatatg aaatcacttt tagatggata actctattta
     1681 gaagtgattt tttatgtagt tattatcata attataagta tgatatataa ggtgattaaa
     1741 ttacataaat aaaggctata atagtatgga tattataaat tatatattga gcagaattac
     1801 ataatttggc gataaatatt agaaatatat at
//
LOCUS       DAKBNN010000358         1778 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000358 DAKBNN010000000
VERSION     DAKBNN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.366"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            85..447
                     /locus_tag="OUB02_003852"
     CDS             85..447
                     /locus_tag="OUB02_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCT8887545.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            883..1584
                     /locus_tag="OUB02_003853"
     CDS             883..1584
                     /locus_tag="OUB02_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HCT8887546.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWY"
BASE COUNT          729 a          142 c          268 g          639 t
ORIGIN      
        1 gcttataatg attatgatat aaattggtta tagtatacaa tttatgggta taacagtaaa
       61 aaattagaaa ggaattttta taaaatgatt aaattatatg gatatacaaa atgctctact
      121 gttaaaaaag ctaaaaattg gcttaaggaa aataatttag aatttgaaga tatagatatg
      181 gttcaaaatc ctcctagtaa agaggagtta aaatcaattt ataagactag tggatatgat
      241 ataaagaaat tttttaatac aagtggtatg aaatatagag aattgggtct taaagatatt
      301 gtaaaaactg aatctgatga taaactttta gaaatattgg taagtgatgg tatgcttatt
      361 aaaagaccat tacttttgga tggtaaaaat gtattattgg gctttaaaga agatgtatgg
      421 aaaagtactt tgctaaaaga agattagaaa gagataggtg tgttgattaa aattattaat
      481 taatacacct ataaatttat gtattaaatc taatttttat aaatattaat tagcaaattc
      541 aaatcaaaaa aattcatatc agcaaacata ttacttctta ttacaaatta tatcatatgt
      601 tttatattaa aatattataa tattacaacg aattgttaca aaattactta ttattaacca
      661 tgaacatttg ttttgtgcta tcatatttat ataaggtaat tatcataaaa aatatcttgt
      721 tcatacagtt ttatatagaa aaaagaaatg aagcttttct ttatataagt atattttgta
      781 aattgcctct atatttatag tttaaataat agtggtatta aaaatctgac aacaaaaata
      841 aaactttcaa aaaattaaat ctaaaaagaa taggagataa atatgagtat tgaagattta
      901 tatgattttg aatgtgttcc tttgaacaat gagattgata ttgatgtttt atttgaaaag
      961 atgatagaag atggaaaaga aaaagaatat acaccaatta tagtcattga tgaaaaagta
     1021 gggctattaa gaaaaaatat agatatgatt actaaagagt atggttctct tgaaaactat
     1081 agagagtatt gtttaacaaa atataatgaa attgatgtaa atcaattttt tgattttagg
     1141 aagagtgaga tttcagaatt attaaaggag ctaattgatt tagaagatga tttttatgat
     1201 tataaacatt ttagaccaat tatgaatata gatatatttc atgaatataa caaagtttac
     1261 atagctaaaa ttccaacaat taaaccatat gaagtatttg catacatacc tattggcgga
     1321 tttaatgatt gtcctagaga tgaagaacat atagctattg caaaatattg gtatgaaaag
     1381 catggaatct ttccaatcgc aattggatgt gatacagttc aatattctgt aaaaaattta
     1441 actaaagatg gtaaaaaatt tgatgactta tgtattgaat tagtatttta ttgtgaggat
     1501 attattatac aagggtatga aaccttaaag gctcttaagg atgtattaaa acgctctaca
     1561 atagtgcttt tttggtggta ttgatattct atcatttaga tagtattaaa ttgttagtca
     1621 ccaagaatcc acttgcttta gttatgggga gtgtgtcaaa ctaggttttt atacacagtg
     1681 tagcatagta atatgtttaa attacaaagg ttaaaaaaat actttaatat ttttaatatt
     1741 ataattaata gatttaaagg taattataat ttataaaa
//
LOCUS       DAKBNN010000359         1772 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000359 DAKBNN010000000
VERSION     DAKBNN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.367"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            150..1052
                     /locus_tag="OUB02_003854"
     CDS             150..1052
                     /locus_tag="OUB02_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HCT8887547.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT          580 a          175 c          365 g          652 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac
      121 tttagtcatg ggaggttcag attggaaatg tgttgccaca agattattat ggattaatag
      181 tgattttagg tgttattctt ggagttatgg gggcatttta caactggttt actctatatg
      241 tgcagtcttt atacaagaag attcctaaaa ttggtacctt tggaaaactg ttgattccat
      301 ttataatggc tggtattctt gggattgtta tgcctgatgt tctggggagt ggacacaatt
      361 tagttgaaga acttacaagt catgaatata tttttggaat ggttgttttg atttttgtta
      421 tacgttttat attttcggct gttagttttg ggtcaggtgc accaggggga atattttttc
      481 cattgcttgt tttaggagca tttataggtg gaatatttgg tatggtaggt gttaaagttt
      541 ttggaatgaa tcccgattat gtaaataact ttgtgctttt ggctatggca ggatatttta
      601 cagctattgt gcgtgcccct ttgactggta tcatattaat ttttgaaatg acaggttctg
      661 ttagtcaaat gctttcacta tcagtaattt caattgtggc atatattgtg gctacactta
      721 tgaagtctaa acctatttat gaaagtcttc ttgagaggtt acttgaaaaa aatggagaac
      781 ctgtacataa agagaggggc gaaaagatac tagaccagtt ctctgttatg tttggctctc
      841 ctgttgcgaa caaggcaatt caggatgtac agtggcctga aaattgtctt ttggtagcaa
      901 ttcaaagagg tggagaagaa attattccta ggggtaagac aattcttaga ccaagtgatg
      961 taattgtgac tatgacagat gagagagata gtggtgctgt gtatgataaa atggaaaatt
     1021 tgtgtaaaga acatacggca catatttttt agggataatg atgaatgttt aataatgata
     1081 gaatatttaa tgaagatttg attaaaaaat tggtaatttg aaagagagtt aaggaattaa
     1141 tataagaaaa tgaaaatgtg atatcttaga tgtaaaagag tttgaagaaa ataattgtat
     1201 atatttgtta atgagattat atggactaat ggtatattta tactgtgcta aaagttgcac
     1261 tattgtatga ggaatgggaa taaagggttt gaaaaaatag gtagaagatt tttcagatat
     1321 tcttattggg atattttaat taatatattt taattgcgat attttgggaa gaatagaatt
     1381 tatttataaa tgaaaataat taaaaggggt taaggttgta ttagcccctt ttgcttatag
     1441 agtttggaaa tggtatcttg atagtagttg tttcatgctt attatatttt attacatatt
     1501 ttaaaattga ccttattact cctaaatcat tgttatatca acttttaaaa cactttcaag
     1561 acaatcgcct attgaaaaca aagttaatta aaaacctaca tatcagttta gtattcgtgt
     1621 agatgattat ataaatataa aagtataaga aaggtgagta tctgtggtta gttcattttt
     1681 aaatgatatc tttagaacaa ttttagtaag ttgtattttt atagttattt tattagtctt
     1741 tagaataact ttatttaaga ttttcagtaa aa
//
LOCUS       DAKBNN010000360         1767 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000360 DAKBNN010000000
VERSION     DAKBNN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.368"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..616
                     /locus_tag="OUB02_003855"
     CDS             <1..616
                     /locus_tag="OUB02_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HCT8887548.1"
                     /translation="TKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRFTLNFF
                     DRQYIIDYPNGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIPGGNVY
                     YPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMVFILWF
                     GDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            643..1281
                     /locus_tag="OUB02_003856"
     CDS             643..1281
                     /locus_tag="OUB02_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HCT8887549.1"
                     /translation="MIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGYASDIRMQ
                     KTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLNKESKLDK
                     AVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCAKKNNRFL
                     EALKILESKMIGNKIVVESVVPKLSKLSFCKKGESSEIAKTHYYEILKNLEK"
     gene            complement(1377..>1767)
                     /locus_tag="OUB02_003857"
     CDS             complement(1377..>1767)
                     /locus_tag="OUB02_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887550.1"
                     /translation="KGFNIDKIDFVYSVKPIDVFLKTFPTLLWYTTFPLMILTLTKNR
                     FTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYMPNNIDVRFNELKNYFFI
                     NRGSLVVISIIIVFYIARKSTVLKKSK"
BASE COUNT          687 a          228 c          230 g          622 t
ORIGIN      
        1 aacaaaaaat aattataatg ttccatttga ctatgttcga aaaaaactca aagaattaga
       61 ccctcataca attagtaatt taagtaatat acctttcaat aatgatttaa atagattcac
      121 actcaatttt tttgatagac aatatattat agactatcca aatggagaag tttggaatag
      181 taatggtagc ctttgcaata actttgagct taaaatcttg atagttagat atttaatcaa
      241 tgctaaaggt attcctagaa ctaataaata tgtaactttt aaagaaatac caggaggcaa
      301 tgtttattat ccaaattttt taaacaggac tctatatagg ctttcagaag tttttacatc
      361 tcaaatagac aaatataagt tagttatgga gaaaattgga gctgaaaaag taaacatggg
      421 agatttagct tataggttta catatatggg taatacatct atggtattta tactttggtt
      481 tggagatgat gaatttcctc caaattcaaa tatattattt gattctaata tagtttatta
      541 cttcaatgca gaagatttag cagttgttcc tgatacagct ataaatgcaa ttttaaataa
      601 aataaatcaa ttataaatat aaataatatt ataataaagt ttttgatatt tagcacatgg
      661 cgagaagatg gacgtttcaa actatatcca aatggaggta tttacccttg tcatactgca
      721 aatgctgcta acgtactctg tcatatggga tatgcatctg acattagaat gcaaaaaacg
      781 tttcaacatt ttttagatac acagtatact gatggtgggt ggcgttgcaa taaatttagt
      841 tttggtcgag gtccagaaac tgaatactca aatccactac caactcttaa tatattaaat
      901 gcattccgtt ttagtaatta tctaaataaa gaatcaaaac ttgacaaagc tgtagatttt
      961 ctacttgacc attggactat taaaaaacca atcggccctt gtcactatgg aattggaacc
     1021 ttatttatgc aagttgaata tcctttccgt aattataacc tattccttta tgtctacgtt
     1081 ttatcatttt ataattgtgc aaaaaagaat aatagatttt tagaagcatt gaagatactt
     1141 gaatctaaaa tgataggtaa taaaattgtc gttgaaagtg tagttccaaa actatccaaa
     1201 ttatcttttt gtaaaaaagg cgagtcaagt gaaatagcca aaacacacta ttacgaaata
     1261 ttaaaaaacc tagaaaaata atagttatat agataaaaca tattaaaaaa tagtggcttt
     1321 aaatttttta aagtcactat tctatataaa tataacaata taaaaaatat attaaactat
     1381 ttactctttt ttaaaaccgt tgattttctt gctatataaa aaactattat tatagaaata
     1441 actactagag aacctctatt tataaagaaa taattcttta attcattaaa tctaacatct
     1501 atattgttag gcatataaaa catcttttga atataaaatc catttacatt aatcatacta
     1561 acaaatgggt acatatgtaa aataaaaaat atatcagcaa atgtatatgt aattaatatt
     1621 gctattgttg taaatctatt ttttgttaat gttaatatca ttagtggaaa tgttgtatac
     1681 cataataatg taggaaacgt ctttaaaaac acatctattg gctttacact ataaacaaaa
     1741 tctattttgt ctatgttaaa acctttc
//
LOCUS       DAKBNN010000361         1667 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000361 DAKBNN010000000
VERSION     DAKBNN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.369"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(316..714)
                     /locus_tag="OUB02_003858"
     CDS             complement(316..714)
                     /locus_tag="OUB02_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887551.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     gene            complement(1070..1555)
                     /locus_tag="OUB02_003859"
     CDS             complement(1070..1555)
                     /locus_tag="OUB02_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCT8887552.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          540 a          231 c          131 g          765 t
ORIGIN      
        1 tacatttctt ttcatataca aaattctatg aataattact ttaatggtct aatagtctgc
       61 tctttggctc taattcttct tatattaatt ttgtgaataa ctgttgatgt aattttatat
      121 tttgattcat gaggaattaa attcttcaaa gttaattaag tacatttatc aataaattta
      181 cttaattaac catcatttaa tactcattcc tgtatttata tgcctccttt gaaacagaat
      241 attactccat tttaattaca tcatattatt aaatagatac caatgattaa aaaaatgata
      301 ttcttttaat tatttttagt aaaatttttt cttctttctt ttaataaata ttagcactac
      361 taaaactata accaaaaata tgactattat ggacaatatt aattttgtta ggatattatg
      421 tcttcttgcg ttttgtcttt tatatttatt tatagtttga tatccatact ttttagttct
      481 tatacttcgt tttttcaact ttactttact tttattatat ttcctacttt tatgtctact
      541 tttagattta atagttttaa atttagtatt atcatatgta taattctctt ttattgaaga
      601 aatgtataca ctagactcaa taatagcttc tgcttctact tgtaatggat ttataatgct
      661 gattataagt agaaaagata tagctatccc aatcaatttt ttcttcttat tcatattttc
      721 cccctcataa aaatttttaa ctagttcatt ataatatata tataactaaa aattacaaat
      781 tttttcaagt ttcttatata tcttattatc tttaatatta tttttattat cataactttt
      841 acttttttta agatttttca aagtagtatt aatttataat acttggtgta taaagtataa
      901 gttaaatcta ctttcaatta aatttaccat ttataattac tttataataa aaataaatta
      961 gttaatttat tctctttagt tctgttataa tgtttctaca aaaaagaatc ccattaatgg
     1021 gattcttttt tgtgatttat taatatattt aaagcttgct agtatcgtat tataaactta
     1081 aatgctcttc tatgtattga ttatatacat atccagaata tcgtttttca tttttattga
     1141 tattaactct tgcccatttt ccaacgctat aacatacttc aactttaaaa ccatcaggga
     1201 ttgtagttat aactttagat tctaatgttc tatcatttct aagttctaaa cttaatccat
     1261 ttgttcttat atttcctatg taattctttt ttctattttt tattgtatca ttactattgt
     1321 ttttagcatt acttaatctt tctaaatatt tataaagtaa ccaacctttg tcataattac
     1381 tctcaacttc tgcccatatt ccatctacac actttaactt aacaatttta ccttctggta
     1441 tactttttaa tttcaaacta tttatgtctg gtttttctct taaattcaat ttaattttag
     1501 tcttaaaatt gatgctagat ttgcgaaaat tttttaacat taattcagat atcatcttaa
     1561 gccctcctct tgccaaatat taagttatta aaatatataa ctctaaaaat acttttaaca
     1621 aaaagaatta ttatcttttt ggcaactggc tgacatgacc tttatta
//
LOCUS       DAKBNN010000362         1657 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000362 DAKBNN010000000
VERSION     DAKBNN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.370"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            442..1647
                     /locus_tag="OUB02_003860"
     CDS             442..1647
                     /locus_tag="OUB02_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCT8887553.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          617 a          210 c          219 g          611 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttctttt tccttttttc
       61 aaggacaagt aatactatat catctttatc tattctagtc aatataattt tttacttttt
      121 ttattttttt tatttatcat gtattcttta tatttaattt ttaaaactta atgctaagta
      181 aacctgttta tttcataaaa ctaatatata ttctatgctc tattttcctt tttattctat
      241 atttaaattt tttatttaca agaatatttt taatataaca tatttgataa aattaattta
      301 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
      361 caataacctt attcacaaag acagttttta tatatcaaaa tataatataa atactatgct
      421 acattttagg aggattttat tatgagcaaa aaagtattaa taatgtctgc ttccactggt
      481 ggtgggcata atagggctgc tctagctatt aaagaagaac ttacttccaa aacacttgat
      541 ggagagccaa ttgaatgtga aataatagat agtttgaaat tagttaataa tacaatggat
      601 aaaataattt caagaggata cgaaaaatct gctatatata ctcccaaagc ctatggaagt
      661 gtatatagat tatctgaaac aaatcttttg tcaaaaaatg aatttaaaga taatctacta
      721 ataacattca tggctaaaaa atttaaaaag cttattagaa gcgaaaaacc agacttgatt
      781 ataggaacgc atccttttcc aatgattgct ctaagtacat tgaaaaaaaa ctttaattta
      841 cataataatg aatcaaatgc ttacacagag catttttata aacattatac aaataccatt
      901 aatgttcctc cgcttatttc tgtattaacc gattatacta ctcattctac atggatacaa
      961 aatgaaattg attactatat tgttggtcat gaatatgtaa aagaattatt ggtgtttgat
     1021 ggagttgaac cgagtaaaat tagaacattt ggaatccctg ttgaaaaatc ttttctctct
     1081 cacagagata aagatattgt tctttctgag ttgaatttat ctcctgataa attaactgtt
     1141 ttacttatgg ggggtagctt tggggctgga aatataaaag agacacttga tgaattactg
     1201 gatactgata gagactttca aatacttgta attactggta agaatgaaag cctaaaagaa
     1261 aaaatcgaaa aaaaacttat gtctcgttat catgataaaa atgtatgtgt tcttggatac
     1321 actgataaga tgaatgatat tttagcttct atagatgtat tagtaagcaa acctggtgga
     1381 cttactacaa ctgaggctct tttaaaagat gtacctatga tagttcctta ttatatacca
     1441 ggtcaggaag aagagaatct tgatttctta tctaattgtg gtgcagcact tagaactaca
     1501 aaaaaatata acttgtcaat attactaaaa gttttaatag atgaccctag cagattagaa
     1561 atgctaaaaa gaaatattaa atcaataaga aagtcaaatt cagcccaaaa tatagctaat
     1621 ttaattttaa atataatttc tggttgataa aataaat
//
LOCUS       DAKBNN010000363         1642 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000363 DAKBNN010000000
VERSION     DAKBNN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.371"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            1..741
                     /locus_tag="OUB02_003861"
     CDS             1..741
                     /locus_tag="OUB02_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCT8887554.1"
                     /translation="MNELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQSKVYE
                     NITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKSKSVE
                     YVLNIKGEKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEILLLE
                     NKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWTKNSN
                     ILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            728..1609
                     /locus_tag="OUB02_003862"
     CDS             728..1609
                     /locus_tag="OUB02_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCT8887555.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT          698 a          190 c          250 g          504 t
ORIGIN      
        1 attaatgaat tacaaaatat aggatttact aaatacgaat ctcaaatata catatcatta
       61 ttaaaggaaa gccctttaac aggatatgaa ataagtaaat tatcaggtgt accacaatca
      121 aaagtctatg aaaatataac aaagctatta aacaataata taattatcaa tgttggaact
      181 gaccctatta agtacatacc aataaaccct aatgaattat taaaaaataa gaaaaaagaa
      241 tttgacaatt ctatggataa tctaaagaat agcttacaga tattaaataa aagtaaaagt
      301 gtagaatatg ttttaaacat aaaaggagaa aaaaatatat taaaaaaagc aatagaaatg
      361 ataaaaaatg caaaacaaga aattataatt tcttgccctt atgaagaaat tcttgaatta
      421 aaaaaagaac tcctaattgc ttacaaaaaa aatattaata ttgaaatact acttttagaa
      481 aacaaaagtc tgaaaggtct tgataatatt catattcatg gaggtaataa aactacactt
      541 gaaaataaaa tgctttacaa aggaattatt ttgattgtag ataatgatga aatattgaca
      601 ggaaacttat cagaaggcag tgaggccatt tcaatttgga caaaaaactc taacatttta
      661 tatattggag ttcaatacat aaaacatgaa atttatattt cagaacaaat aaaaggaggt
      721 tataaaaatg aacaaaatta agattaaaca agttgatgct tttacaacta taccttttgg
      781 tggaaatcct gctggtgtcg ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa
      841 tgtggctaaa gaaatgaatt tatcagaaac tgcatttgtt tcagagtcaa atattgcaga
      901 ttttaaagta caatttttca caccaggttt agaagttgat ttatgtggtc atgcaacaat
      961 aggtactttc catgcacttt tggaagaagg gaagcttaat actaataaga gtatatttta
     1021 tcaagaaaca aaagctggtg tattatctgt agagcttaag aaaataaata atgaaaatat
     1081 atttatgatg gaacagaata cacctgaatt tgaaaattta gataattgca gaaaagaact
     1141 tgcacaaatg ataggtgtgc atgaagatag acttcttgaa tatcctatta taaaggtaaa
     1201 tactggactt tggtggcttg tatttggtct aaaatcttta gatgatttaa aaaatatctc
     1261 tccagattta aataaaatca aaaaatttag tggtgataat aatataattg gtataactcc
     1321 attttgtata gaaactatag ataaaaagtg tgactaccat ttaagatcta tagcaccata
     1381 tgttggagtt acagaagacc ctgtatgtgg aactgggaac gcttgcgtat catcttatat
     1441 agtacatcac aatattttag ataaaactag ttttattggt gagcaaggta actttataaa
     1501 cagacctgga aaggtatatg taaatataac tagagaaaac aatgacataa cttctgtaaa
     1561 aattggagga agtgcttata ctgttttaag tggtgaaatt atgtattaat cccataccta
     1621 ataaaaacaa ataaaaatac ta
//
LOCUS       DAKBNN010000364         1628 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000364 DAKBNN010000000
VERSION     DAKBNN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.372"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            292..1563
                     /gene="serS"
                     /locus_tag="OUB02_003863"
     CDS             292..1563
                     /gene="serS"
                     /locus_tag="OUB02_003863"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCT8887556.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          685 a          167 c          295 g          481 t
ORIGIN      
        1 agttaataac tatacaagat tgataaagaa aaagtcaaaa tagataattg attagcaaaa
       61 tagatataat tataatgaag ttaaatctag caatattctc ctaaaattaa atttcattta
      121 tttaattaaa tataatttga caatgggcaa aaaagtgagt aaaatataaa taaaataaaa
      181 ataacaggtt cggataaaga aaaaggtttc ttaacctcaa gcatgaagta tattatctaa
      241 gttatttgag attaagagat aaatccaatc ttaggtactg aggaggaaaa aatgcttgat
      301 ataaaaagaa taagagaaaa tttagattgt ataaaaaaag caatggaaag aaggggcgaa
      361 aaagacttta accttgatga agtagttaag ttagatgatg aaagaagaaa gattcttcaa
      421 gaagttgaag ttatgaaaaa tgaattaaat acagcatcta aaaatatacc aaatcttata
      481 aaagaaggta aagatgtaga aaatgaaaag attaaattaa aagagttatc tgataaaatt
      541 aaggtaatag accaaaatct taaagaagta gaagataaga tggaatacct attaatgaga
      601 ataccaaatg taccacatcc agaagtacca caaggtgaaa ctgatgaaga taatgttgaa
      661 gtaagaactt ggggaaagac tactaccttt gattttgaat ctaaggctca ttgggaaatt
      721 ggaacagaat taggtatact tgattttgaa acagctagta aaataactgg ttcaagattt
      781 actctttaca aaggattagg tgctagatta gagagagcat tattaaattt ttacttagac
      841 acgaatacaa aagtaaatgg atatacagaa gttattccac catttatggc aaatagaaat
      901 agttttttag gtacaggaca gttacctaag tttgaagaag atatgtttaa aatagaaggt
      961 atggactatt ttatgattcc aacatcagaa gttcctttaa ctaatataca tgctaatgaa
     1021 atacttaaat ttgaacaatt accgataaat tatacagctt atactccatg ttttaggtca
     1081 gaagctggtt ctgctggaag agatacaaga ggtttagtaa gacagcatca gtttaacaaa
     1141 gtagaaatgg ttaaaatagt tgcaccagaa gaatcttaca atgagttaga aaagcttact
     1201 aacaatgcgg aaactatgct acaattattg aatctgcctt atagagtagt aaagatatgt
     1261 acaggtgatt taggatttac agcttctttt aaatatgatg tagaagtttg gatgccaagt
     1321 tacaataggt atgttgagat ttcatcttgt tcaaactgcg aagatttcca agctagaaga
     1381 gcaggaataa gatttaaaag agataaagat tctaaagctg aatatgtaca cacattaaat
     1441 ggctctgggt tagctattgg aagaagtgtt gcagctatat tagaaaatta tcaacaagaa
     1501 gatggttctg tggttgttcc tgaagtttta agaccatata tgggagtttc tgttattaaa
     1561 taagagttaa attatgtatt tttaagcaat acaataatat agaggtaata aaaaataatt
     1621 aatataaa
//
LOCUS       DAKBNN010000365         1616 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000365 DAKBNN010000000
VERSION     DAKBNN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.373"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..974
                     /locus_tag="OUB02_003864"
     CDS             <1..974
                     /locus_tag="OUB02_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCT8887557.1"
                     /translation="KIIEDKNDLALESYYSSVIEGAFSNRKIAKSIIRGKMKPSNKSE
                     YMIYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGE
                     IIHIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALS
                     YLYLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSIN
                     EMRNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRY
                     LKAGIKEIELVDQLMEVFNNLEGIEILSKEKNIYKVNDKYLKMLDID"
BASE COUNT          676 a          129 c          245 g          566 t
ORIGIN      
        1 ttaaaataat agaagataaa aatgatttgg cactagagtc ttattattct agtgtaatag
       61 aaggtgcttt ttcaaataga aagattgcaa aatctataat tagaggaaag atgaaaccta
      121 gtaataaaag tgaatatatg atttacaata atcatagagc cttagaatat ggattagaca
      181 acttagataa attatatagt cacaaattta tatatgattt gcatcatata ttgggcgaaa
      241 actgcttgga ttcagaagaa tatgagtata gaactgaaaa agtatatgta tgtgattcaa
      301 aaggtgagat tatacatata gggcttgaac ctttgaagat atatgacttt atgagtaaat
      361 taatagattt tatggaaaat agcaaagtaa gtaatttaat taaaagtgct ataattcatt
      421 tttactttgt atatgttcat ccttttagtg atggtaatgg aagaacatca agagccttat
      481 catatctata tttgattgat aaaggatatg atactttcaa ggagttttca atatcatata
      541 tgatatctaa aaacagaact aagtattata aggctatact tgatgtagaa aacaaaggaa
      601 ataatttaac tgtatttata gagtttatgc tcaagtctat aatacaatct attaatgaga
      661 tgcgtaatat gcatgataga aaatctttag aaagtatatt aaaagaagaa ttgtttgaaa
      721 atgacataac tctatcagca acagaagaac atatattaaa atatatttgc aataaagata
      781 actattctat gacattagaa aattatataa aaaagaataa aagtagatac ttaaaggctg
      841 gaataaagga aattgaatta gttgaccagt tgatggaagt gtttaataat cttgaaggga
      901 tagagatttt gtctaaagaa aaaaatatat ataaagttaa tgataagtac ttaaaaatgc
      961 ttgatataga ctaatttaga taatctggag agggaatata taaataataa gaaaaaggca
     1021 tttttgattt ctggggctat aaacgtaatt tcattaatta ttgttttttc attgtttcat
     1081 tataatatta tagaaaatag tacacaaaat atgataataa ctgtaatctt aatttttatt
     1141 tttgaaatga taaaaataaa aatcatagag aaatactata aactataaac aagaaatatc
     1201 ttttctaaga gcatattgta caatatgctc ttaatttagt tatatagatt taatataaat
     1261 aatgtatttt ttgtagatga tttatgtaag atttatgacc aatttgtgta tgatttgtgt
     1321 tctttaatca tgacaatatt atattgtaaa aaataaattt aataaaaaat gacttggaat
     1381 ttatattcta ggtctttttt atataaaaat ttaataagtt tcaaatgttg gtaactttga
     1441 cagtagaaaa cgtctattga gataacgagg attctaattg agggggagat atattagcac
     1501 ttaaagtagt atatgcaaca gatataatct ttataaaagt aattcgaatt aaaatgtaaa
     1561 tataaagaat aattaaaaaa atatcgcaat tatttgtttt tcatatggat aaaatt
//
LOCUS       DAKBNN010000366         1607 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000366 DAKBNN010000000
VERSION     DAKBNN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.374"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            145..1425
                     /locus_tag="OUB02_003865"
     CDS             145..1425
                     /locus_tag="OUB02_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCT8887558.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSSISFSLDDTIFKTLISY
                     DIKATNIYNLIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          674 a          200 c          177 g          556 t
ORIGIN      
        1 aaccagtttt ttatgtaggt aacatttcta catttttata aattttttta cgttttttaa
       61 ttttataatt aaaaatttaa attttataca tattataata aattgtgata tatataaaat
      121 aattaaagat aaaaggagta ttttatgaac atacttggat taatagttga atataaccca
      181 tttcataatg gacacatata tcatttatct aaatctatag aaaaaacaaa tgcaacacat
      241 actgtagcaa taatgagtgg aaattttcta caaagaggcg aaccagcttt atttgataaa
      301 tacactagag caaaaattgc tgtcgaaaat ggtgtagact tagttataga attaccaact
      361 ttatttgctt gtcaaagtgc tgaaattttt tctcatggtg ctataacaat tttaaattca
      421 ctaaactgca taaattctat atgttttggt agtgaagaag gaaatacaga aattctttat
      481 aatatagctg aaattttagc aaatgaacca aatgaattta agttactttt aaaaaaatat
      541 ttagatgatg gtctgttatt tccaacagct agagctaatg cactttttga ttatatacat
      601 caagacaaaa aaaataattt tatacaaaat tattcaaagg aacaattatc aaaaacatta
      661 aattcatcaa acaacatact gggtatagaa tacataaaaa acctcatcca tttaaacagt
      721 agtataaaac catttactat ctccagggtc caatcagaat ataattccga agaaattgag
      781 agtgatattt gttctgctac tgctataaga acttcactta aagaaaatac agaccttttt
      841 catttaaaaa atgttattcc agaaaaaact tataacatcc taaatgaaaa aatagataat
      901 ggttttttac ctatgtttga tgacttcttc tttgatattt taaaaacaat tatacttaga
      961 gatatggaca ctttaaaaga gtattttgaa gtaaatgaag gcatagaaaa cagaatctat
     1021 aaagatatct ttacatctga atttctacat gaactacaat tagcaattaa atccaagcga
     1081 tacactttaa ccaaaatcaa acgaactcta aacaatatat tgctaggtat taaaaaaagc
     1141 gacatgcaac ttataaaaaa catagatact ataccttata taagaatact tgcttttaat
     1201 gataaaggta gagaaattct aaaagagata aaaaataaat ctgaaataaa aataataaac
     1261 aagttttcaa gtatatcatt ttcattagat gatacgattt ttaaaacact cataagttat
     1321 gatataaaag ctacaaatat atataattta atatattata aaaacaataa agatttagta
     1381 aaaggcccta tggattttta tactcatcca atatatgtaa agtagttttt taaaataact
     1441 atatttataa aaatcatttt attaactctt tattatataa gatacaatag tcttacttta
     1501 tttatgtaca catgctttta ggatgacaac atcaacttaa atattatatt actttctaaa
     1561 tttaagtaaa tattgccatc ctaaaaattt tacaatttag cttcaga
//
LOCUS       DAKBNN010000367         1538 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000367 DAKBNN010000000
VERSION     DAKBNN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.375"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(27..1364)
                     /locus_tag="OUB02_003866"
     CDS             complement(27..1364)
                     /locus_tag="OUB02_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCT8887559.1"
                     /translation="MGIYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT          592 a          189 c          193 g          564 t
ORIGIN      
        1 attatataag ataatctaat tttagactaa aataatctaa tataaagaaa taaagaaaat
       61 tcactctcag tataagagat atccaatttt ccattgtact tattaacaat ttctctaata
      121 ttctcaatac catatccgtg agaattacta tcttttttat ctgaaataaa tagttcttgt
      181 tttctattga taggatttac cttgttgttt gtcaataaat agctaaaata ctccttctca
      241 caacgtgcct ttaataccac ttttctattt gacaaatcag gaattttcaa agatgcttct
      301 atagcgttat ctaaagaatt tgaaaaaata gtacataaat ctataggttc tatacccaag
      361 tgattgtcta tatctatatt aaagtgcaca tcaacaccat tttcttctaa acttgtatga
      421 cgaagattta aaattgagtt aagagcttgg tttttgcaaa attgttttcc actaagagta
      481 cctataccta tttttaactg attaaaatac tttttagctc cagcaatatc ttcaatttct
      541 aaatacgatt caatcatttg aaagtgatta ttcatatcat gtcgcatttt gcgtatttga
      601 atttgttgat tttctagtaa gttatagtat ttttctttta ggtgatttgc ttgtttttct
      661 atcttcatct ttacattttc aatcaatggc tgtaaaagaa gtatcacacc gatatttgcc
      721 agaattgctg acaagatgat tagataagtg ccataaggta agttgcctaa gataatattc
      781 ttattactac ccatagggat taaattagta ggtggcagaa taatagcaat taatattgat
      841 aaaaatgaag atatacatac catatcaata aaaatccata ttttatttga taagtattgt
      901 tttatattga ccaatctatt ctttaggaaa atatgtatta aaaaccaaaa taataattta
      961 attagagcag aaattatctg gactattgaa agtattaaaa tatactcttt tgagtgtata
     1021 agttcctcat atatagggtt gtttataaaa agaaaattaa gcccaattat aataggataa
     1081 agtatcatga caactgatat tataggaatt aatttgcttt tgctacatat tataagtagt
     1141 ataaaatatc caaatagtgt aaacaaaata tttacggggt cggaaagcca tattatcatt
     1201 tgactaataa tagagtagcc caaaatcaat atgattttga aaatccaatg attcctaaag
     1261 tctataaaat aggaagtcaa cttataaaga aaaaaggcta tgaaaatatt gattaatatt
     1321 agaataaaat ttatgcttgt agagattaaa gaataaatgc ccatttaata tacctttcta
     1381 ttaattaaga ttctggaaac taagttttaa aaaattaaat cttaaaaatt aaaattctgg
     1441 aaattagatt tcaaagaatt gattttgaaa aataaaattc tagaaattaa gcttcaaaaa
     1501 attgagtttt aaaagattga aattttaaaa attaagtt
//
LOCUS       DAKBNN010000368         1533 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000368 DAKBNN010000000
VERSION     DAKBNN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.376"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            241..486
                     /locus_tag="OUB02_003867"
     CDS             241..486
                     /locus_tag="OUB02_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HCT8887560.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(704..973)
                     /locus_tag="OUB02_003868"
     CDS             complement(704..973)
                     /locus_tag="OUB02_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HCT8887561.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          617 a          218 c          173 g          525 t
ORIGIN      
        1 ataattttct tgaaggtgaa ttttatatat taaattaaac ataaagtatt ttatttgtat
       61 aaaactatat tatgatgtaa ttaagtgact cagtgattta gaggtggtaa aatatatata
      121 tatgaaagag cataaataaa ttgatataat aaaaaattaa atattaaata ttaactataa
      181 taaaaaaata tctggtaata ggtataatat aaaaagaata taccagaaag aaggtgaagt
      241 atgggagaac tacaaaaaat tccaggggta gggaaagcaa cagaaaaatc tttaatcatg
      301 ttaggatata caactattaa atcacttaaa gatgcaaacc cagcacaaat gtatgaaaag
      361 gaatgtttaa tgcgtggaca gcacatagac agatgtcagt tatatgtata cagatgtgca
      421 gtatattttg catcaactga aaatccagaa ccagaaaaat taaagtggtg gtattggaaa
      481 gattaaaaaa tcaaaaaagt tagacataaa aaagagttga atatccgttt taaataataa
      541 aaactaatac tcaactcttt tttagtcgta aattttatgt aaatctattt gtatggctaa
      601 atatacccat caataaatgt ataaatatac taatcaacaa atgactaaac atactaccta
      661 acaaaggatt aaataaattc cttaacaaag cattctaaaa caattaatta gaaactgttt
      721 cttcaagcca ttttctacat tgctcaatat ttaccatagg gtcaaaacct tggtcttttc
      781 ccttatttaa aagcatatca caagggaaag tatcacattc accacaatga ttaagttcct
      841 tactttcaca gcaagtttta accttacatt ctccacccca aaatggtttt tccatagttg
      901 ggcaaccagt gcaatttact tgttcttttt ttgtacaact gtcacaacag acaccacatc
      961 ttgattcaaa catattcaaa catcctttcc attatttaaa aatattataa aatgatttta
     1021 gatgaataca aaagtgcaaa tagttgattt taggtcatct aaatgttaaa cataaaatta
     1081 ttagtactgg taaatttata aaaactattc caaaaatctt tttgaatatt tcaaaacaca
     1141 ataaattact tcaaatcccc ttgaatatat tttacagcat tatagattgc tttaaatctc
     1201 tttgaatatt ctacaagact atagattagt ttaaatccct ttgaatatat tttacaacat
     1261 tatagattgt tttaaatctc tttgaatatt ctacaagact atagattagt ttaaatccct
     1321 ttgaatattt caaaacacca tagattactt taaatccctt tgaatgttct ataacactat
     1381 aaattacttt taacttttat ataaccataa agtttttatc tggcacttta aatggagttt
     1441 tatatcaaat ttgccttcca tcgaataacc aaacttaatt taattacaca ttccaagatt
     1501 caaatattca ttcaatatat ttgataattc tat
//
LOCUS       DAKBNN010000369         1521 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000369 DAKBNN010000000
VERSION     DAKBNN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.377"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            39..1277
                     /locus_tag="OUB02_003869"
     CDS             39..1277
                     /locus_tag="OUB02_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HCT8887562.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFYNKDTMKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          645 a          137 c          265 g          474 t
ORIGIN      
        1 gttactagca attattagta gaaatggggg aaatagagat gaataaggga ttttacaaat
       61 taaattcagt ggtattgaac tttgatatta agaatgatgt taacaagata agatttaatc
      121 attatataat gagaaaagaa aacatacaaa gacttcaagg aactctgcca aacggacaat
      181 tttatatgac agtgagaaga gctgctacag atttacaact ttctatttct actataagta
      241 ggcttgtaaa agagtttttg gatttaggga taataagatt agtgtctaag ggtgtaaaag
      301 gtaatcgttc tgtatatagt tacgtaagta tagagtttaa tggaagttta tttgatgata
      361 gtaaatatat ggaacttgac tgtgtatgta atgatgaatt tattgataat agtggcatag
      421 agtttgattg tgatagcaaa gataattctt ttaatggaga tataaatgta gaaactgtgc
      481 attgcattaa tgagtatttc tctgatgatg atgtagagca taattataac aatttctata
      541 acaaagatac tatgaaggat aaagccattt ctaatattag cactatcaaa aaatatattg
      601 atgaatcttt tgatacaaat aaatcagaaa attctaactt acaacaggag tcaataaatt
      661 tcaactttaa tgatacatgc aatgaaatag ataaaagaac gaataatagt atagaaaata
      721 atcaaaataa aaggcctatg agattgagtg acatcagtgt tttaagtgat aaaggtatga
      781 caaataaaaa gaattacttt aatcataatc atggtataca aaaagggact aaatctgtaa
      841 caaagaaaaa agaatcctta aataaattta ataaaaaaaa tatacagcaa agcattataa
      901 atatgctaaa taaaaaatca ggtaagaatt ttaaaatcga ttctccaata agcataaaac
      961 ttatcaatga aaggctaaag gaaggctatg tcttagaaga tttctacaaa gttattgagg
     1021 taaaagtggc taagtggaaa aataccataa tggaaatgta tataagacca gaaaccttgt
     1081 ttagctataa gtttgaatcc tatgtaaatg aaaacatatc tatgaaaagt caaagtaatg
     1141 ttcacccatg taaagaaggc aatagtaatg atagcagtaa ttatatacat tctgattata
     1201 caacatatga tggcagtgtg cgtgagagaa aaaaatatga gaatgtaaga tactggaatc
     1261 ctatatgtga aatttaatta gtttaagaat gtatttatga taaataagat attaatagag
     1321 aatagtgagc tatagcaaat gatatatcaa tagttggtag taagttatat taagcaagat
     1381 attactatca ggtagtgaat tgtattgaac gatatatcaa taattggtag taaattatat
     1441 taagtaagat attactatca ggtagtgaat tatattgaac gatatattaa taattggcag
     1501 taaattatat taaataagat a
//
LOCUS       DAKBNN010000370         1519 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000370 DAKBNN010000000
VERSION     DAKBNN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.378"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            140..874
                     /locus_tag="OUB02_003870"
     CDS             140..874
                     /locus_tag="OUB02_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8887563.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1017..1508)
                     /locus_tag="OUB02_003871"
     CDS             complement(1017..1508)
                     /locus_tag="OUB02_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCT8887564.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          546 a          183 c          230 g          560 t
ORIGIN      
        1 atataaatca tattcattta tattattaaa aaatttattt atagaaactt ttattatttt
       61 atataaaagt gataaaaact tatattttgt gtttagtaga gtgttgctaa gtgaatatat
      121 ttaaaatgga ggaaacttta tgaaacaaaa taaatatgat gatgatagat tttttaataa
      181 atacagtaaa atggaacgtt ctacaaatgg attagcaggt gctggagagt ggcatgtttt
      241 aaaaaaaatg ttacctgatt tcaaaggaaa aagagtttta gatttaggat gtggatttgg
      301 atggcattgt caatatgcag ttgagaatgg agcaatttct gctgttggag ttgatatttc
      361 agaaaaaatg ttaaaagaag ctagaaacaa aacgcaactt gataatataa attacatatg
      421 tatgcctatt gaagatatta attttccaaa agatagtttt gatgtagtta ttagttcctt
      481 ggcatttcat tatattcaat catttgaaga tatatgtaag aatataagta attgtctttc
      541 aaataaaggt aattttgtat tttctgttga acatcctata tttactgcat atggaagtca
      601 agagtggtac tgtgataaag atggcactaa tctacactgg ccagtagata gatattttga
      661 agaaggagta cgtaagtcaa attttctagg agaagaggtt ataaaatatc ataaaacatt
      721 aacaacttat ttaaatacgt taatgaaaac aggatttgaa atattagaaa ttattgaacc
      781 tcaaccagaa gaaaaactgt tagatacaat tcctgatatg aaagatgagc tacgtagacc
      841 tatgatgtta cttgtatctg caaaaaaaag ataagtaaat ataaataatc attattgtag
      901 aaaaaaagtt gtagaaaata attatactaa tggagatgat tataccagtg gtattaatta
      961 caccagtagt aaggaggcaa aagcattcgt tgcttttgcc attttttaag ttgcaattat
     1021 acttctacgt agtatatttc gtttccattt tcttctataa gctttttcat atcttcaaat
     1081 tttaaaaata cagtagcagt attatcattt ggatgtatac ctattttatc catttttaat
     1141 aaatccttat caaatacaac ttttacaaca gcctctttat catttaatat accaaaagga
     1201 gtcacagctc ctttagtaag tttcaagtat ttttgaagtc tttcttctga tgcaaaacta
     1261 agaggtttac tatcaatttg ttgacgaata tcttttaaat ctgctttttt gtctttacct
     1321 aatacaacta aaaaatgttc ttttccttta gcatcacgaa gaaataggtt ttttacaaca
     1381 aattccatat gtaatgtttt taattcttcc atttcttcaa ttgtataaac tggtggatga
     1441 tttacagcct cataaggtat gcttaactca tctaattttt tatatacttc ctgttgttga
     1501 ttgttcatga tttcctccc
//
LOCUS       DAKBNN010000371         1477 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000371 DAKBNN010000000
VERSION     DAKBNN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.379"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1426
                     /locus_tag="OUB02_003872"
     CDS             <1..1426
                     /locus_tag="OUB02_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCT8887565.1"
                     /translation="DTGIKDKYKELFLKIEELDTILNLSKDGILFTSKDGEINTYNSK
                     VKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDKVVVFNKKYINVNKKNIYNRDEK
                     MGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAKKVSKS
                     DLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYDKGTFT
                     GGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVINIDVR
                     IISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINKKLIIT
                     PEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSLKLKYS
                     KNDILNILEILILNKESDVGIGRGLILKALLDKNLQITEGKIKKIFEYLKKEELIICS
                     SGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT          678 a          150 c          220 g          429 t
ORIGIN      
        1 agatactggt ataaaagata aatataagga attattttta aaaatagaag aattagatac
       61 aatattgaat ttatcaaaag atggtatatt atttacttca aaagatggtg aaataaatac
      121 atataatagt aaagtaaaag atattttaga cataaatgag gatatatatg gaaaatacat
      181 tgaggatata tttgtagata gtttaaaagt tttattgagt gagaaagaga tactagataa
      241 agttgtcgta ttcaataaga aatatataaa tgtaaataaa aaaaacatat acaatagaga
      301 tgaaaaaatg ggcacatatt atagtttaca agaaataaca tacataaaaa aattagaaca
      361 aaatctcact aaaaaattaa gagaaaaagg acaaatagca aaatatacat ttaaggatat
      421 aaaaacaaat agtcctaaaa tgtttgaatg tatagattta gctaagaagg tttctaaatc
      481 tgatttaagc atacttatca gaggtgaaag tggaactggt aaagaactca tagctcagtc
      541 tatacataac aactcaaata gaaaaaatca accctttata gcagtaaatt gtgcagccgt
      601 tcctgaaaat ttattagaaa gccagctatt cggatatgac aagggaactt ttactggagg
      661 tctaaaagat ggaaaacaag gattatttga acttgctaat aatggaacaa tattcttaga
      721 tgaaataggc gatatgcctt tagaattaca aacaaaacta cttagagttc ttcaagaaaa
      781 gcagataatg cctgttgggt ctcacaatgt aattaatata gatgttagaa tcatatctgc
      841 tacaaataag aatttagaac aaatgattga taatagccaa tttagagagg atttatatta
      901 tcgattaaat acaattccaa taaatatacc tcctctaaga gaaagaaaag aagacatatt
      961 aattattatg gaagatttaa ttaataagaa attaataatc acaccagaag caaaaaaact
     1021 tatacaaaac tatatgtgga aaggtaatat aagagaactc caaaatgtta catcctattt
     1081 aaacattatg tgtgaagata ttgtattaga gaaagattta cctccaaact taaggtcttc
     1141 agataacaaa aatacatcat taaaactaaa atatagtaag aatgatatat tgaatatatt
     1201 agagatacta attttaaata aggaatcaga tgttggtatt ggtagaggat taattttaaa
     1261 agcactactc gataagaacc ttcaaattac agaaggaaaa attaaaaaaa tatttgaata
     1321 tctaaaaaaa gaagaactta taatttgtag ttcaggaaga tatggaagta aaataactca
     1381 aaaaggagaa gatttttaca ataaattaaa atataaaggt ctataaaatt ataatttatc
     1441 aaacaaagaa atctattcag gattaaagta ctccttc
//
LOCUS       DAKBNN010000372         1467 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000372 DAKBNN010000000
VERSION     DAKBNN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.380"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            114..854
                     /locus_tag="OUB02_003873"
     CDS             114..854
                     /locus_tag="OUB02_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCT8887566.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            866..>1467
                     /locus_tag="OUB02_003874"
     CDS             866..>1467
                     /locus_tag="OUB02_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCT8887567.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLV"
BASE COUNT          663 a          100 c          253 g          451 t
ORIGIN      
        1 caattaataa ccaaatacta aaaaatattt taaataaaga acttaaaatt gtgataaaat
       61 taatttatag atattaaaaa aagtataata tatggttttt aagaggtgct aagatgaaaa
      121 attttagaga tttaggtggg aacaaaactg aggatggcag aactgtaaag aagggattat
      181 tttataggtc tgcaaaatta tcaaatctaa gtgaaaatga tataaagatt ttaaaagagt
      241 taaatattaa atatatattt gattatagaa gtgatgaaga agctcgtaaa catccatcaa
      301 caataatatc aaatataaaa aatataagaa taccagcaat gagagagcta gaagaatcag
      361 gaggaagttt tggttctatt gaagatatga ttgatggatt gtttgaaaaa gatggtgcat
      421 ttaatatgtt aaataatagt tactataatt tgcctataaa taatccgtca tataaaaaat
      481 tagtagaact tataagagac tattctaatt taccaatatt aaatcattgt acagctggta
      541 aggatagaac tggtgtgggg agtgctataa ttcttatgat tttaggggta tcgagagaga
      601 atataatgaa agattatcta aaaagtaatg attttgcaga taaagagata gaaagattta
      661 tagaatataa gcctaaattt aaggacattc caaaagaaaa tttgaaatat atatttggtg
      721 taaatgaaga gtatatgaaa actgcattta gaagaattga tgaagaatac attagtgtag
      781 aagcatattt atatggagaa tttaatttaa ataaagaaga aataagaaaa ttaagaaatc
      841 aatatctaga gtaaaaggtg agtttatgag tttaaatata acagcaagac agataaatat
      901 aataaaaata ttgttaaatt caaaagatac aattagtggt attgccttaa gtcaagagat
      961 aggatgttct tcaaaaacta tacaaaatga aataaaggat attaataaac aacttagaaa
     1021 tggtaaaatt ttgtcaataa gaggagttgg ttataaacta gaaggaagtt ttgatgaggt
     1081 aaatctaaat agcaatctct atgatgatgt agatagagta gaatatataa tcagaaaaat
     1141 attaacctta agtaatgaag aaaaaaatac tataaaactt gaagatttag ccgattctat
     1201 gtatgtaagt gtttcaacag taaaaaatga tttaaaagaa gtaaaaagaa tattggaaaa
     1261 gtattcaata tcagttaaat ctaagcataa acaaggtata tgtgttttgg aaagtgaaga
     1321 taagatatta aattgtatag ttgacttaag caataagaga gataatcaat tgagtttaaa
     1381 tgatttttta aatgatgata taaaggaaag aaaactatta ataaaaaaat tattattaga
     1441 catattaaat gaaaatggat tggtatt
//
LOCUS       DAKBNN010000373         1390 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000373 DAKBNN010000000
VERSION     DAKBNN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.381"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1320
                     /gene="cdeA"
                     /locus_tag="OUB02_003875"
     CDS             <1..1320
                     /gene="cdeA"
                     /locus_tag="OUB02_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HCT8887568.1"
                     /translation="NLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVGSD
                     ALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIVGI
                     LFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGNSK
                     FSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKSTLK
                     LRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLYAH
                     FLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFAPF
                     IVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSRAF
                     VFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
BASE COUNT          449 a          176 c          200 g          565 t
ORIGIN      
        1 aatttattta caagaaaatt cactactttt gaatttctaa aatttgtttc tcctgcaatt
       61 atatccatga tatttatatc tttgtacaca ataatagatg gcatctttgt atcgacatta
      121 gttggttctg atgctcttgc tagtataaat attgtactac ctataattaa ccttgtttgt
      181 ggatttggaa taatgatggc aactggtgga ggtgctatcg tttctatacg tatgggtgaa
      241 aatagacagg atgaagccaa ctctacattt tcttttatag ttttgttttc attgattgtt
      301 gggattttat tcacagtaat ctcatatttc tttatcaaag aaatatctat tttgcttggt
      361 gcaacagata agttattacc atattgtata acttatggta aagttatgat tttatgtaca
      421 ccattttata ttttaaaatt tatatttgag tactttgcaa gaactgatgg aaattctaaa
      481 tttagtttat ttctatcagt cattggtggt gtaacaaata taatattgga ttatgtattt
      541 attaaatatt ttggaatggg tcttttagga gctgcagttg caactgctat aggtattatt
      601 ttaacttgtg ttttaggtat tatttacttc ttatctaata aatctacact aaaattaaga
      661 aaaccaaaaa ccgattttag acttataaga gatactatga taaacggttc ttctgaaatg
      721 gttacagaat tatctacagg aattacaaca ttcttattta atgtagtagc tttaaaatta
      781 gcaggagaaa atggacttgc tgctcttact atagtattgt atgctcattt tttaatgaca
      841 tcagtctatc taggattcgc tgctggagtg tctccattaa taagctataa ttttggtgct
      901 gaaaacagtg ataaattaaa agaaacattt aaacattctc taaaatttat atttgtttct
      961 tctcttttag tgtttattat tgctttagta tttgcaccat ttattgttag ggtctttgta
     1021 agtccagata acacagtatt taaactagcc ttacaaggat taaaaatatt tgcatttgct
     1081 tttttgtttg ttggtataaa tatatttgca tcaggatttt ttacagcatt tcacaatgga
     1141 aaaatttcag ctattatatc ttttagtcgt gcctttgttt ttataatcat aggaatcata
     1201 attcttcctc ctatgttaaa catgactgga ttatggctta cagttccatt tgctgaggtt
     1261 ataaccatat ttatatctat tctatttata aaaaaatata aatgtagata taagtattaa
     1321 aaattaatac ttaaaatcaa catttattaa aactaataaa cctgctttaa aatacaaatt
     1381 tacaaatttg
//
LOCUS       DAKBNN010000374         1387 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000374 DAKBNN010000000
VERSION     DAKBNN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.382"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            22..957
                     /locus_tag="OUB02_003876"
     CDS             22..957
                     /locus_tag="OUB02_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887569.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            971..>1387
                     /locus_tag="OUB02_003877"
     CDS             971..>1387
                     /locus_tag="OUB02_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887570.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFY"
BASE COUNT          564 a          165 c          202 g          456 t
ORIGIN      
        1 aattattttg gagggattac tatggatata gtcagagtaa ataatataac aaaaagattt
       61 aatgataaat tagtacttga taacatcagc ttttctgtta aaaaaggtga gatatttgga
      121 cttattggcc caaatggtgc aggtaaatct acacttataa atataattac aaatcttatg
      181 ttaccaaata gtggtagtat tcaaatcaat ggtttagatt tatcaaaaga ttatataaaa
      241 gcaaagtcta taatagggtt agttccacaa gaattagcaa ttatagaaac tcttactcct
      301 tttgataact tagagtactt tggagctttc tacggtttaa agggaaaact acttaaagaa
      361 agaatcattg aagcattaga agttacaggt cttactgaag tcaaaaagaa aaaggtcaaa
      421 aaattatctg gtggtatgca aaggagatta aatattggaa tagcactctt aaatcatcct
      481 aaaatattaa tattggatga accaactgta ggtgtagacc cccaatcaag aaatcatatt
      541 ttcaatttta taaaagatat atctaaaaaa catgaaacta ctgtaatata tacttcccat
      601 tatatggaag aagttgaaca cctttgttct aaaattttta ttatggatga aggtaaagaa
      661 attgcttttg gagataatga ttatttaaaa tcactcgtat ctacaaatac aaaattgatt
      721 atggaaataa aaaatataaa tgcccaactt atctttgatt taaaaaatac taaaggtgtt
      781 atatctgttt tagaaaataa ttcattatta gaattggata tagataaaaa attacaactt
      841 acagatatac tctctgttat tgacaaaaac aattcaaaaa taatgaaaat ttcttatgaa
      901 gaacctagcc tagaagacgt atttttaaat ctaacaggaa aaaatttaag ggactaatgg
      961 aggtgatttt atgaaattat acatgagtgt aaaaactatg ctaaagagtt taaaaagctc
     1021 ctttatactt aatttaatat attttttagc tttgcctctg attttatctt ggtttcttgg
     1081 gatggttaca gaaagcatgt ttcaaaaccc tataaaaaca gaatctaccc ccattgttat
     1141 atatgacaaa gataacacaa gattatcaaa tgatttgaca aagtatttga aaaatgattt
     1201 atcttatata ttaacagtta aaaaagatga ttctaaggca gaattaaagc ttactatacc
     1261 taaaggctat gagagtagtt tattaaatga aaaatcaaat actctaaaca tagaaaaact
     1321 tggaactcgt gatgatattg caatattgct tcaagatata ttagatactt accatgaaaa
     1381 attttat
//
LOCUS       DAKBNN010000375         1373 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000375 DAKBNN010000000
VERSION     DAKBNN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.383"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            209..919
                     /gene="rgaR"
                     /locus_tag="OUB02_003878"
     CDS             209..919
                     /gene="rgaR"
                     /locus_tag="OUB02_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HCT8887571.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          543 a          113 c          210 g          507 t
ORIGIN      
        1 tttattttac tatatttttt tacataatat atctagaaaa gtataggaat ttatcgtaat
       61 ttaagtttaa atattaaaga gaatttctat aagtagttta atcgacaatt taaatttata
      121 aagaaattta aatttgaaag ctagtgagta cagtttggaa attgaaataa agtaaactaa
      181 atgatatttt agatttggag gtgatattat gttcagggta gttatatgtg atgacgaaaa
      241 aatacaaaga agcatactaa aagagtttac agaaaaactt ttgtctgaaa gatgtctaaa
      301 ttatcaaata ctggaatttt cctgtggaga agatttaata tccaaatacc cagaaaaaat
      361 tgatattata tttttagata tccaaatgaa agatatcaat ggaattgagg tggctaaaaa
      421 aataagaaag tttgatgaaa aagtagaaat tatatttaca acggcttttt cagagtatgc
      481 cccccaaggt tatgaggttc gtgcttatag atatttagta aagcctatag agtatagtaa
      541 ctttgcaatg ggtgtaaatc tttgtattga taaccttcaa agaaagaagg agcgttatat
      601 agttttaaat agtaaaaaag gatttaatag aatactgata gattctattt tatatgttga
      661 aactgtaaag agagatttag tcgtacatac aataggtaga gattataaag ctaatatgag
      721 catgaaacaa gcagaaaaat tactgtgtga aaatggcttt tttagatgtc atacaagttt
      781 tattgttaat ttaagaagaa tagaagatct tagggataat atgattacta ttaatgaaca
      841 atttatacct ataagtaagt atagacttaa agatttaaaa gtagcattaa caagcttgtt
      901 atgtgatata atgtattaat tttaaaaagg taattgaaaa tatcgtaaaa aagatttaat
      961 gtcatcacag ggctattgat tgaaactgtc ttaatatata aaatgttatt tatattaaga
     1021 cagttttatt atgcaaaata gtagtttata gatttttaat atggtttaac atgtatatgt
     1081 agagtttcca ttataatagc atgattatta attagtagct tgattatcaa tatactatta
     1141 ttgattttta tatgcattat atgtattttt agaatatgat taccttttta aaataatgac
     1201 tatttataaa actctataga tatttgtttc atttagctac gcatacttta attaattata
     1261 ttaatattta atggagtaca atataaatat taaagcgaca tcttatagtt tattgaacct
     1321 atagtaatga attaataaat agtttaattc tatgaaaaga tttcctttta tgc
//
LOCUS       DAKBNN010000376         1372 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000376 DAKBNN010000000
VERSION     DAKBNN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.384"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(232..543)
                     /locus_tag="OUB02_003879"
     CDS             complement(232..543)
                     /locus_tag="OUB02_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887572.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(599..1192)
                     /locus_tag="OUB02_003880"
     CDS             complement(599..1192)
                     /locus_tag="OUB02_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCT8887573.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          492 a          196 c          171 g          513 t
ORIGIN      
        1 atagaaaatt aatctaaaga agtataataa actaatttaa gaaatataga aaattaattt
       61 aaagaaatac aatacattaa tttttaagta acattaaatt aatgtaagta aaataatgtt
      121 catatgaata tttaaaggtg cattttctga agctaagtat atgttgtaga gtgttatgtc
      181 tctctatcct gtgaagttta atagctcaag aaaatgcact atttatgatg attattttgt
      241 tttatttcca attttggaaa taactattct aattaaatct aaaattatac cataaataag
      301 aaaagaaagt atagatgcta tgtaccaata aatcgaaatt gaccctgtaa ttgttctaca
      361 tgggatttgc atacctgaat ccataaaaac aaaaaaacta tcaactctgg aaataataga
      421 caaaatagga tttaaaccta ttagaacaat actttttgag tcttgaccaa tttgatgcat
      481 ataaatctca aataatgcaa ttaaaaaaaa ccaagatgta aaagatttaa taattttttt
      541 catcaaaata cctcacataa tataaattct gtttgcacta tttgcttact acaactattt
      601 atctccattc agtcacatca cttaattgta atctaggtct tcttgatggt tcttcatcag
      661 gataacctaa agatattgct gccactaatt gactatctgt atttaaccat tcacataatt
      721 cacgatatgc aaagtataca tcacaaatcc aaagactacc tagacctaat tccacagctg
      781 taagcgacat attctgaatt gcagctccaa ttgattgcat atttgccatt tcatagaatc
      841 gttcttcagg ggatagcttt tctaaaggtg atttacctaa tatatttaaa ataaaaattg
      901 ttactggagc ttgtttcatg atttcaactg tgtaatttgc accagcgata tgttgacgac
      961 ttcctggaag aagcccatta tcattaattt cattttgaat acctttgctc atggctttaa
     1021 gcatagattc tttttccttt tcagtgatga caacaaaacg ccatggttgc ctgtttttag
     1081 atgaaggtgc atttattcca gcttcaatta ttttctctat agtttcatgt gaaatgcttt
     1141 gatttttata ttttctgata cttcgtcttt ttgatataga atcacttatc attttaagta
     1201 acctccgttt attttttgga aaaatctatt taatttttat tttctatttt aattatttta
     1261 gcattaatca gaaaaaaagt cattgatttt agtatacaat tattaatgat tatattaaga
     1321 attgtatcaa aaagtatgtg attatactaa gggattatat taaacattat at
//
LOCUS       DAKBNN010000377         1365 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000377 DAKBNN010000000
VERSION     DAKBNN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.385"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            217..1029
                     /locus_tag="OUB02_003881"
     CDS             217..1029
                     /locus_tag="OUB02_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HCT8887574.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1118..1309)
                     /locus_tag="OUB02_003882"
     CDS             complement(1118..1309)
                     /locus_tag="OUB02_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887575.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          491 a          164 c          199 g          511 t
ORIGIN      
        1 tttatatgac taagttctat aactaaattg aaataatcac tttttcaaga atgttataat
       61 ttttttattg tattcttttt gctatagtct atgaataaat caatagaaaa tcttctaagt
      121 tgcttttaaa taatgacttt atggttcaat tgtactaaaa tagtatatgt aggttacaaa
      181 taatctaata aatattaaag tttggaggta atagatatgg cagattgtaa ttcatgtcca
      241 tcaaagggaa attgtaatag tcaatcaaat tgttctattg aaaataaccc taacaataaa
      301 tttggaaaaa taattggtgt tatgagtggg aagggtggag ttggaaaatc aactgtaact
      361 gctttacttg ctaataagct aaacaaaatg ggatataagg ttggtatatt agactcagat
      421 ataactggac caagtattcc tagacttatg ggtgtaaaaa atgtaaaagc ttattctgat
      481 ggctcttata tttaccctgt agaaaactca aataacataa aagtaatgtc tataaacctt
      541 atgatagatg atgaaaatga acctgttgtt tggagaggac ctttacttgg aggggttgta
      601 aagcaatttt atactgatgt actttgggaa gaattagact atttattaat agatatgcct
      661 cctggtactg gagatgttgc cttaacagtt atgcaatcta ttcctataag tggaatagtt
      721 atggtttcag tacctcaaga tttggtatct atgattgtat caaaagctgt taatatggct
      781 aaaaaaatga acataaatgt ccttggagtt attgaaaata tgagttatat ccaatgtcct
      841 gattgttcta aaaaaatcaa gttatttgaa ggagaatcta ctgaaaagtt tttagatgat
      901 ttagacttag aacttttagg agaacttcct atgactaaag aaattataga tattactcat
      961 aatggagtta cagaaattag tgatgattta gattcaatat tgactaatgt tgttgaaaaa
     1021 attaaataaa taaaaatttt ataattttat atttatcaaa aaaagaggct tgtatttatt
     1081 ttataatcat acaaataaat ataaacctct tttatgttta tctatgtttt aaatacacaa
     1141 aatattcttc tacttcatct ccatttttta cttttactaa atattcatta taatgagcat
     1201 tatatggctg tgtcattttg cattctatta cattttctat gtcttctaat tctaaaagtt
     1261 ctacatcctt taaaaatttt ctttctatga gttccattaa tctttccata atatatcctc
     1321 acttccccta taattaagat aaactatata tcctcttaat acaga
//
LOCUS       DAKBNN010000378         1359 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000378 DAKBNN010000000
VERSION     DAKBNN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.386"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            198..1295
                     /gene="ychF"
                     /locus_tag="OUB02_003883"
     CDS             198..1295
                     /gene="ychF"
                     /locus_tag="OUB02_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCT8887576.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          534 a          148 c          243 g          434 t
ORIGIN      
        1 aaaagcaata tttttattta aattaaatac aaatattatt taaatcaata tgctttaaag
       61 tctagtttac aagcttgata tatatgatgt ataatcaata tgtatgtcta taattaaaac
      121 aattttgcaa tcctatagat tgattgttta aagttatata aagaatataa aaaagaataa
      181 gagagaggac gattaatatg aaattaggca tagttggttt accaaatgta ggtaaaagca
      241 cactatttaa cgcaataact caagcaggtg cagaatctgc aaattaccct ttttgtacaa
      301 tagacccaaa tgtgggagtt gtatctgttc cagatgaaag attaaacaaa ctacaagaat
      361 tatataattc tgaaaaaata gttcctactg ctatagaatt ttgtgatata gcaggtttag
      421 ttagaggagc ttctaaagga gaaggtttag gaaataaatt cttatcacat ataagagaag
      481 ttgatgcaat agttcatgtt gttagatgtt ttgaggatga aaatgttgta catgtagatg
      541 gttctgtaga cccacttcgt gatatagaaa ctataaactt agaattaatt ttctcagata
      601 tagagattct tgaaagaaga attaataaaa ctcaaaaagc tgctaaggct gataaaactt
      661 taggttctga acttgattta ttaaaatcaa taatgtctac tttagaagaa agtaagtgtg
      721 taagaactat ggaatttaca gaagatgaac aaacattcgt aaattcattg gatttactta
      781 catctaagcc tgtaatatat gcttcaaatg tatcagaaga tgatttagct gataatggag
      841 aaaataataa atatgttcaa caagttaaag catttgcaga aacagaagat gccgaagttg
      901 ttgtagtatg tgcacaaata gaagcagaaa tttctgaact tgattctgct gaagaaaaga
      961 aagagttttt agaaacatta ggtcttgaac aatcagggct tgataagctt ataaaatctt
     1021 cttatgcttt acttggttta atttcattct taactgctgg tccaaaagaa gttagagcat
     1081 ggactataaa agttggaagt aaagctcctc aggcaggagg aaaaatacat tctgatatag
     1141 aaagaggatt tatcagagca gaaacaatag cctttaatga tttggttgag catggtacta
     1201 tggctgctgc taaagaaaaa gggttggtta aattagaagg taaagaatac atagttaaag
     1261 atggagatgt aatattattt agatttaatg tttaaaatga ttaatcgtat acactttgac
     1321 tattatatat aataactaaa aaactaagat tataataat
//
LOCUS       DAKBNN010000379         1337 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000379 DAKBNN010000000
VERSION     DAKBNN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.387"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            27..101
                     /locus_tag="OUB02_003884"
     tRNA            27..101
                     /locus_tag="OUB02_003884"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="OUB02_003885"
     tRNA            116..200
                     /locus_tag="OUB02_003885"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="OUB02_003886"
     tRNA            210..293
                     /locus_tag="OUB02_003886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="OUB02_003887"
     tRNA            323..399
                     /locus_tag="OUB02_003887"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="OUB02_003888"
     tRNA            407..482
                     /locus_tag="OUB02_003888"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="OUB02_003889"
     tRNA            571..659
                     /locus_tag="OUB02_003889"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="OUB02_003890"
     tRNA            663..738
                     /locus_tag="OUB02_003890"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="OUB02_003891"
     tRNA            745..818
                     /locus_tag="OUB02_003891"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="OUB02_003892"
     tRNA            833..909
                     /locus_tag="OUB02_003892"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="OUB02_003893"
     tRNA            933..1009
                     /locus_tag="OUB02_003893"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="OUB02_003894"
     tRNA            1017..1093
                     /locus_tag="OUB02_003894"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="OUB02_003895"
     tRNA            1102..1177
                     /locus_tag="OUB02_003895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="OUB02_003896"
     tRNA            1185..1258
                     /locus_tag="OUB02_003896"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="OUB02_003897"
     tRNA            1265..1336
                     /locus_tag="OUB02_003897"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          320 a          309 c          356 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtggctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAKBNN010000380         1326 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000380 DAKBNN010000000
VERSION     DAKBNN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.388"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            45..980
                     /locus_tag="OUB02_003898"
     CDS             45..980
                     /locus_tag="OUB02_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8887577.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINHEI
                     KKYGRRFKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGNGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
BASE COUNT          552 a          112 c          240 g          422 t
ORIGIN      
        1 atataaatta atatactgaa taaaatcaat ctggagggat aattatgaag tgtacaaact
       61 ttactttcaa aggagaggaa ggtttagata tatatacata taaatgggaa gatgaaaata
      121 taaaaaaacc aaaggcagtt atccaaattg cacatggaat ggctgaaact gcacaaaggt
      181 atgagacttt tgccaaggta cttactaaaa atggttatat agtatatatc aatgaccata
      241 gaggtcatgg aaaaactgca aagataatag aaaatgttgg acatttagct gaaaaagaag
      301 gatttagatg tcttgtagag gacatgtata ctttaacaaa tattataaag aaagaaaatg
      361 aagatttgcc aatttatcta tttggacaca gcatgggttc atttgcaagt caaagatata
      421 taatggatta cagtaataat ttatctggac ttatactatg tggttcaaat ggaaagcaag
      481 gaatcattct aaatcttgct catctgataa tcaatcatga gattaaaaag tatggaagac
      541 gttttaaaag taataaaata aataatttga tatttggcgg cgaaataata agaagaaatg
      601 aaaagactaa gtttgactgg ttaagtagag ataaagaaca agttgaaaaa tatataaatg
      661 acccattttg tggagtagtg tgtagttgtg gcttttttta tgatttagtt caagggttaa
      721 aagaaattga ggataaagaa aacttaaaaa aagtgccact tgatatacct atttacataa
      781 tatctggaga taaagacccc ataggaaaaa atggtaacgg agtcttaaga ctaagggata
      841 gatatataaa attaggagta aaagatgtaa cttgcaaact ttacaaagat ggaaggcatg
      901 aattgttgaa tgaaataaat agagaagaag tttttgagga tataatttgt tggttaaata
      961 ataaaataga aatattgtaa taaaaaaaat ataatgatat atttacttta aatttattta
     1021 tatataatat ttgaaaagtg gattatgtat atgcaaagta gaaaagagag agcctagaag
     1081 tgatttttcg ctacaatgcc atttattgaa taggtgggtg ttattataaa taaattatta
     1141 acactcagta ttattatcat tgtatatgga atttatttta tttaaaattc cttgaaagtt
     1201 taaatatagt aagttgtagt aaagagaaaa tctaaatatt taaatgctat ttaatgtgaa
     1261 tctattaatt aggttatact taaacttaat taatagattt tatatttcaa gctaatgaga
     1321 tagggt
//
LOCUS       DAKBNN010000381         1314 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000381 DAKBNN010000000
VERSION     DAKBNN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.389"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            81..1262
                     /locus_tag="OUB02_003899"
     CDS             81..1262
                     /locus_tag="OUB02_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCT8887578.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          521 a          133 c          277 g          383 t
ORIGIN      
        1 atagtggtaa aattaattat tagaatatta gaaataataa ataaataata cattgtactt
       61 aaaaggggga gaaatttaag atgaacaaaa aatatattaa agcagggaat ttaattgatg
      121 gaaccggaaa tagtattatg caaaataagg gtataattat tgatggagat actatagttg
      181 aaattgaaga tataagagat gatttaaatg gatatgatgt gtacgattac ttagataaaa
      241 ctgttatgcc aggcattatg aattgtcatg ttcacttgac aatggagcca gttggaaatc
      301 caaagatata ttatgataat gtatcagatg tagagcttgt agtaaatacg ataaaacaac
      361 ttgatgctta tttggattca ggagtaacat atattagaag cttaggttgt cccaaatatg
      421 tggatgtaca acttaaaaat cttatagaca aagggtctat agatggtcct ggtatagtta
      481 cttcaggtcc agttatatgt atgacaggag ggcatggata ttattttggt attgaaagtg
      541 atggagtgga tgaatgtaga aaatcagcca gaacagtttt aaaaaatgga gtagattgtg
      601 taaagataat ggcaactgga ggtgtaacaa ctgatggagt tgagccaggg tcaccacaat
      661 taactttgga ggaaatgaag gctgctgtag atgaagctat aaaagcaggt aaaataactg
      721 ccacacatgc acaaggaaga actggtataa aaaatgcagt gcttgcagga attacttcta
      781 tagaacatgg tgtttattta gatgatgaaa taattgattt gatgcttgaa agaggaactt
      841 acttagttcc aactgtggct gcaccatatt ttattctaaa tgcaggaaaa gaaagtggaa
      901 taactgaagc tactcttaga aagtgtgaga ttgttgccat tccacacaaa gagtcattcc
      961 taaaagcata taaaaaagga attaaaattg cagttggttc tgatgcagga acttcttata
     1021 atgaacatgg aaaaacatat tatgaaatga aactaatgtc agattatggt atggataata
     1081 tggatataat agttgctgct acaaaaacag catctgaact tttaagaatt gataaaaatt
     1141 atggaactct agaaaaagga aagaaagcag atgttatagt tgtaaatgga aatccagtag
     1201 agaatataga cgtacttgca gatgtgttag cagtatttaa acttggtaaa aaagttagat
     1261 aaatagtata aaaataaaaa agagtgtcta aagaaatttt taaataatta taga
//
LOCUS       DAKBNN010000382         1249 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000382 DAKBNN010000000
VERSION     DAKBNN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.390"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            106..1095
                     /locus_tag="OUB02_003900"
     CDS             106..1095
                     /locus_tag="OUB02_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCT8887579.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLRMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          458 a          122 c          240 g          429 t
ORIGIN      
        1 ataaacatat agtattaatt ttaaattcaa caatttaatt gttaaaagtg ttattattaa
       61 attagagata tcttaactta atatactgag atgggagtga tagatatgtt aaaagaagaa
      121 aaaacttatc aagagaaaga cgcaaaagta ttcaatggag tggcagcttt gataatgatt
      181 ttattatttt tagtagtttc aataggtatc atggtttttg ggatagtaaa acttgatagt
      241 ggatttactg gaataggttc acttatgtta attttgggac ttgtatttat agtagtagat
      301 tttatcctat tttttgggct aagaatgatt aatcctaaag aagctatagt tctagttttg
      361 tttggtaact attatggaac tataaaaaaa gaagggtact attgggtgaa tccattttgt
      421 tcagcaataa acccagcagt atcaagaatt aatgtatcga aatcttcatc tgatgaaaag
      481 gtagatatat caactaattc aaggggcaaa aaagtatctt taaaaacaat gactcttaat
      541 aatgaaaagc agaaagtcaa tgatttattg ggaaatccaa ttataatagg agtggttgta
      601 atttggaagg ttatagatgc aacaaaggca gtatttaatg tggataatta taatacattc
      661 ttatctattc agtgtgattc tactattcgc aatgtttcaa gactttatcc atatgatgta
      721 tctgaagatg gagatgaaaa atctcttcgt ggtagtagtc aggaaatagc agatagatta
      781 aaagatgaat tacaatcaag ggttgacatt gcaggaattg aagtgtgtga agtgagaatt
      841 actcatcttt cttatgcacc agagattgca gcagcaatgt tacaacgtca acaagcagag
      901 gctataatag cagcacgtaa aaagattgtt gagggggctg taagtatggt agagatggca
      961 ttgactaatt taagtgaaaa taatgttgtg actttggacg aagaacgtaa agcagctatg
     1021 gtaagtaatc tgttagttgt gctttgtggc aataaagatg cacaaccagt agtaaacagc
     1081 agttctattt attaaattat aaaaagttta atagtttatt ttattgatat atagaaaatt
     1141 tagtagttca ttttattaaa ttatagaaag attaatggtt cgctttattg aattataaaa
     1201 agtttaataa tttattctat tgatatatag aaaatttagt agttcattt
//
LOCUS       DAKBNN010000383         1235 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000383 DAKBNN010000000
VERSION     DAKBNN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.391"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(24..962)
                     /gene="pdaA"
                     /locus_tag="OUB02_003901"
     CDS             complement(24..962)
                     /gene="pdaA"
                     /locus_tag="OUB02_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HCT8887580.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT          415 a          163 c          165 g          492 t
ORIGIN      
        1 gactttagat gtaattataa atattaatac tccaagagtt caaattcata tcctagtttt
       61 cttgcatttg atataaaatc atttaaaatt tctgtactag ttttggaaac tgcatgtagt
      121 aacaagatag aaccatcatg tagattatcc attatttttt gcgttgcttc ttcatgtgat
      181 ggttgtttat cagtatccca gtctctgtat gcaaaactcc aaaatacagt cttgtatcct
      241 aaatttttag tcattgccaa gcttttttct gagtattttc ccataggagg tctaaaaaat
      301 ttaaccatat ctttcccagt tacatcttta tataactttt caacatcata taattcgtca
      361 ttaaagtttt ttagattgct tgtttttgtt ggcatagatg gatgggtttt actatgattt
      421 ccaactatat gtccctcaga aaccattctt tttactaaat ctttattttc ttttatatat
      481 ggagctgtaa caaaaaaaac tgctttaacc tgattttgtt ttagagtgtc gagtattttt
      541 gtagtgtatc cattttcata accttcatca aatgtaagat atagtgtttt ttgaccatct
      601 tttgtaggtc cattgtatag ggcatcatag tcagaaaatt taaattcaat gtccgtattt
      661 acttctggtg ttttatgctt attgtttggt atataaaacc aatcaagagt agtgttatct
      721 aaagaagata catctaattt attattatca ttattagtag tggcttttga tggtttatct
      781 gttccgtctg aaactattac attggaagta tctttaccac tatctatttt ttccttatct
      841 tcttgtaact gtgtttcctt attttgattt tcgttttgat tgttttgtga atttgaacat
      901 ccaacaataa aaaaaataga aatagaaagt agtgttaaat aatgcaatac ttttctaaac
      961 attttgaagc tcctttctaa attaataata attgcatata ataatgatta tatgattaat
     1021 tacaattgca tatagtatta ttataattgg aaattactat taataatagt tatatcttta
     1081 aaaataaagt cacaatagta tgacaaaggg tgaataatca ataaatttaa aaaaaaatga
     1141 aaaaaatagt tgaccatatt atgagaagat gttattataa taaatgtgct caacaagaag
     1201 taacaaaata acaagcacaa agaagttaat tgaat
//
LOCUS       DAKBNN010000384         1222 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000384 DAKBNN010000000
VERSION     DAKBNN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.392"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            228..1172
                     /locus_tag="OUB02_003902"
     CDS             228..1172
                     /locus_tag="OUB02_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCT8887581.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT          430 a          125 c          221 g          446 t
ORIGIN      
        1 cagtctattt taactttaaa taagttatat ataactcatt ttacaattaa aataagtcta
       61 aatcaactta aagtaagtga aaaataaata taaaaaaatt gaaaatttat tagtattatt
      121 ggaaagacta gggttttaga gtcgatatat attgatttaa gatggcatag tttttgctta
      181 aaaagaagag tgtaaattga ctaaataaaa atagaaggga atttaaaatg ggagaaaaaa
      241 gtaaagggta tgtatttata gcaatagcag ggttattatg ggctacactt ggattatttg
      301 gaaagttttt aatgggaaat ggactgacat cagaacaagt agcatttaca agattgtttt
      361 ttggatttat agttttaggc gtatattcat ctataaggac accacaaatc ttaaagataa
      421 gcaaaaaagg aattatatat tcagtaataa taggtattat atgtcaggca atgtttaatt
      481 tatgttattt taaagcaatt gacattgcgg gtgtatcaat tgcagcagtt ttactatata
      541 catcaccact gtttttagca atattttcaa aaatatgtta taaagaaaat attactagaa
      601 gcaaattatt ttcacttatt ctttgtttta ttggtgcaat tatggcagtg actggtggac
      661 gtttggattt tcaaggttta aacgcttttg gacttttact tggggtatta tcagcaatag
      721 catatgcact tatgccaact attagtaaaa atgctttaaa ggaattttca agttcaacaa
      781 tattagtata cagtttctta tttggagcta tatttatgat tccttcttca agaccttggg
      841 aaatcttaaa ttatgctaag gatttagatg ttttatcttg tatgctaatg ttaggcattg
      901 ttccagctgc acttgcatat attttttatg cagcaggtat atctaaaggt gtagagttgt
      961 cagttgcagg tgttgtggca tcagtagaat tagttggttc agtaataatt ggttgcacta
     1021 tattaggaga aagtttttcc ttagggaagt tatttggagt gatgttgatg ttaatatctg
     1081 cagtagtagc acttaatctt tcttatgatg aaataagaat attttataag tccaacaagt
     1141 taaagcagat tgaaaaaact gaaagtattt agaattatat acttaaaaag gatttattgg
     1201 tatacatttt agagtccaac aa
//
LOCUS       DAKBNN010000385         1194 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000385 DAKBNN010000000
VERSION     DAKBNN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.393"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(144..947)
                     /gene="proC"
                     /locus_tag="OUB02_003903"
     CDS             complement(144..947)
                     /gene="proC"
                     /locus_tag="OUB02_003903"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCT8887582.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT          386 a          224 c          120 g          464 t
ORIGIN      
        1 taataaattc tcactcaatc attaaattcc tttttatagt aaattactct tattttactt
       61 ataccaaata catttatgta tagatataaa taagcactaa aaaaacaatt caagaaacca
      121 tatagtaaac ttaattacat ctgttatttg ctcatatctt tagacttatc tatacaagca
      181 acaatagatt ttataactgc tgctctaaag ccttcttctt ctaatacctt aacagcttct
      241 atagtagttc ctgctggaga acaaaccata tcttttaatt cacctggatg ttttccagtt
      301 tcaagaacca tcttagctga ccccattact gcttgagatg caaatttata agcttgttgt
      361 cttggcattc cagctaatac tgctgcatct gccatagctt ctataaacat aaatacatat
      421 gcaggagaag aaccactaat acctgtaact gtatccatta aatattctga tactatttct
      481 gcctcaccaa aagattcaaa cagagcctta atcatattta actcttcatc tgttacatta
      541 ctatttttac aaagagctga cattccttcg ccaacaaggg ctggagtatt aggcattact
      601 cttactattt ttttatctga acctataaaa ctttccagac tttctatagt tttacctgct
      661 gctatagata ctattatttt atttgcatct attaattctt taagtggagt taaagccttt
      721 tcgcatatat taggtttaag tgctactatt accacatcag aatttgtaac agctaccttt
      781 gaatcagttg tagtatttac tccaaattct tcttttactt tatctaaggc aacttggctt
      841 aaatctgata cagttatatc ttctgccttt actaatcctg actgtactat gccacctatc
      901 attgcacttc ccatattccc tgaaccaata aatcctaaag ttttcatatc aaatctcctt
      961 ttttctatta gttttatttt aaagtagcta aattgacttt attttaactg cttaaaatta
     1021 tcttattttt ataaaatata accttattat aactataata aattattctt ttgtatgtat
     1081 tacttactaa tatgtgtatg atttactaaa tataaattta acgatttttt cattaaactt
     1141 aacgagctaa cttcctattt agaaagttag ctcataatta atctaaaatt ttaa
//
LOCUS       DAKBNN010000386         1171 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000386 DAKBNN010000000
VERSION     DAKBNN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.394"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     misc_binding    58..321
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            472..1023
                     /locus_tag="OUB02_003904"
     CDS             472..1023
                     /locus_tag="OUB02_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HCT8887583.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          459 a          125 c          192 g          395 t
ORIGIN      
        1 gacatttttt agaaaaaaga atatcattat tttaacaaca aaattcaata caataaatta
       61 aatcctatga caaggaagag tagttaaatt taggttcagt atagagagct gaggatggtg
      121 gaatcttagc aagaaaaaat ttgatgaatg gcccttagag gaataaattg aaatcatagt
      181 tttgagagta gactttatcg tcagtcgcac gttatagcga ataaggtatg taagtacctg
      241 ataatgagat gtatgaaaat tgaattttat attaattttt atataattta ggtggcaacg
      301 cggataatct ccgtcctatt agtttaggac ggagtttttt attttactga aaatttaaaa
      361 gatagaaggt ggtgagaaag atgttatgaa tttcaaaagc taaagatgat gatatagtag
      421 gttggcaaaa aaataaattg aaaattaaaa aaataggagg aagtacaaaa aatgaaaaca
      481 aatacaaaaa aattaacttt aaatgcaata cttttagcga tgggattatt aatacatcaa
      541 atcacaccag ctataggatt accaatgaag ccagatgtac cactagcaat gttatttgta
      601 atattggttt taaatagaga tgattataaa acttgtttga tagcgggtat agtaacagga
      661 atatttacag cacttacatc aagttttccg ggaggtcaaa ttcctaatgt aatagataaa
      721 actcttacag caaatatagt atttttatta atgaatatta gttataatat gccttttata
      781 aaaaaattag ctaagaaaac acaagatact gtagtagttg caataataat gccaatagga
      841 actcttgtaa gtggaactat ctttttatta gcagctcaag ctatagttgg tcttccaggg
      901 gcttcattta cagctttatt cttagcagta gtattaccag ctgttttaat aaatctagtt
      961 gcaggaatat ttttattcaa gattgttagt ctatcaataa gaagagttag ttatcaagct
     1021 taaacacgaa ataatatttt actttataaa gcattataat aactaaataa cataagaata
     1081 aagtagatta taactatttt attcttatgt tgtttaaaga tttggtatta tatatttatt
     1141 tttaataaaa tatttatata gtaccaatta c
//
LOCUS       DAKBNN010000387         1161 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000387 DAKBNN010000000
VERSION     DAKBNN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.395"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            219..974
                     /locus_tag="OUB02_003905"
     CDS             219..974
                     /locus_tag="OUB02_003905"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCT8887584.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
BASE COUNT          448 a          127 c          179 g          407 t
ORIGIN      
        1 tcatggggca ctctttttgg tatgaagtca aatatatttt tatcatttaa acaatgaaaa
       61 agaaatttat ctatattttg aaaaatccac ttaaattttt taatgtaaga taaattgtaa
      121 gcttattatc actaatatta ttttatataa attatattag tgataaaata aacttataga
      181 aatataacaa atttttatta atttcatagg aggaatttat gttgaaagtt ataattagtg
      241 gctataatgg aacaatggga agagttttag cagaatgtgt aaaaaaagat aatgaattag
      301 agttggtttg tggtgtagct aggtctgctg atgaaaaagg tcatcatagt gatacaaagc
      361 tttacaaaac aatgactgat gtaactgaaa aaactgatgt cataatagac ttttcacatc
      421 atgagacttt agagccaata ttgtcatatg ctctaaaaaa taatacacct gtagtattag
      481 ctactactgg atatgacgaa aatgacttaa ataaaataga agaagcatca aaaaacattc
      541 ctatattatt atcatcaaat acatctcttg gagtaaatat attaataaaa ttagttaagg
      601 atgcttcaaa gatgttagaa ggttttgata ttgaaatcat agaaaaacat cacaatagaa
      661 aagaagatgc ccctagtgga actgctacta tgatagctaa tgcaattaga gaagtcttgc
      721 ctgaaagtga aaatacacat ggtagatatg gtagagattg taaaagaaaa ccaaatgaaa
      781 ttggaataca ctctataaga ggtggcaata taatcagcga ccatgatgtt atatttgcag
      841 gtgataatga agttcttaca ttaagccatc attctcaatc tgatgaagta tttgcaaacg
      901 gttctatagt tgcagctaaa tttttaattg gtaagcaatc tggactttat aaaatgtcag
      961 atatactagg ataagttttg tacataataa atctcaatct atttaggtat tcatattcat
     1021 caatttgaat atctaattga ttgagatttc tattctaaaa gtttataaat tttatgctta
     1081 ccttttaaat ataatcattt ttcttaaaga ttatcttatt tattggtata tggaattata
     1141 ttgtttatta ttttataatc t
//
LOCUS       DAKBNN010000388         1146 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000388 DAKBNN010000000
VERSION     DAKBNN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.396"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            55..1122
                     /locus_tag="OUB02_003906"
     CDS             55..1122
                     /locus_tag="OUB02_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HCT8887585.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
BASE COUNT          493 a          113 c          198 g          342 t
ORIGIN      
        1 actctaaaaa agtatatttt atttaaaaat ttattaatac aaaggagatt tactatgaaa
       61 aataaattag tttacatatg tattatgagt ctattgctat ttgtagtagg atgttctaat
      121 aatccaaata aggaaagtac cattaaaaaa gactctaaga ataaccaaag ccaagttaaa
      181 gaagaaaaaa gagaacctgt gaaaaaagat ataactataa gtttcgctgg tgatgtaact
      241 atggggaact ataaaggttc atcatattat ggcagttttg accatgaatt taaaagacaa
      301 ggagaagatt acaattattt tcttaaaaat gtaaaacctg tatttgaaaa agatgactta
      361 acagtggtta atttagaagg tccacttact acagcgtcaa atgcaaaaga aaaaaaattt
      421 gcatttaaag gaaagcctgg atatgtaaat attttaaaat ctggaagtgt agaggcagta
      481 agtattgcaa ataatcattc agaagattat tttgaagaag gaatgaaaga tacacagttt
      541 atattggacg agaataaaat aaattatttt ggattaggga aagatgctgt tgtagatgtc
      601 aaaggaatta aagtgggtct attagggtat aatggattat caactgaata taatgaaaaa
      661 aatttaaaac aaatggaaga tgatattaag tccttaaaaa agaaatcgga tgttgttgta
      721 gtgtactttc attgggggat agaattagat tattatccag ataaaaaaca aaaagatttt
      781 gctcattatg ctatagacaa gggagcagat ttagttatgg ggtctcaccc gcatgtgatt
      841 caaggaatag aaaagtataa aaataagtat atagcttata gtctagggaa tttttgcttt
      901 ggaggaaata agaatccttc tgatacagat agttatatat atcaacaaac ctttactttt
      961 tcagataata aattaacttc aattaaagag ccaaatataa taccaacttc aataacatct
     1021 agtaactcca gaaataatta tcaaccaaaa gtattggatg gaagtgagaa ggatagagtt
     1081 ttaaataaac ttgaaaaaat aagcaaagat ttaaataaat aaaatgtaaa aaaaaataaa
     1141 tatata
//
LOCUS       DAKBNN010000389         1133 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000389 DAKBNN010000000
VERSION     DAKBNN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.397"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>1133
                     /locus_tag="OUB02_003907"
     rRNA            <1..>1133
                     /locus_tag="OUB02_003907"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          299 a          250 c          342 g          242 t
ORIGIN      
        1 ggatgtgatg gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag
       61 tctctatcga ggttaaaggt acccgtaccg caaaccgaca caggtgggtg aggagagtat
      121 cctaaggcca gcgagagaac tgttgttaag gaactcggca aaatgacccc gtaacttagg
      181 gataaggggt gccaccatca ggtggccgca gagaataggc ccaagcgact gtttaccaaa
      241 aacataggtt tctgctaagt cgcaagacga tgtataggag ctgacgcctg cccggtgctg
      301 gaaggttaag gggatctgtt agagcaatcg aagcagtgaa cttaagcccc agtaaacggc
      361 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
      421 acgaaaggcg taacgatttg ggcactgtct caacaacaga ctcggtgaaa ttgtaattcc
      481 ggtgaagatg ccggatacct gcgacaggac ggaaagaccc catggagctt tactgtagct
      541 tgacattggg tcttggtact acatgtacag gataggtggg aggctttgaa accaggacgc
      601 cagttttggc ggagccatcc ttgggatacc acccttgtag tactgggact ctaaccatag
      661 gccatgaatc tggtcttggg acactgtcag gtgggcagtt tgactggggc ggtcgcctcc
      721 caaaaggtaa cggaggcgct caaaggttct ctcagtacgg tcggaaatcg tacgtagagt
      781 gtaaaggcaa aagagagctt gattgcaaga catacaggtc gagcaaggat gaaaatcgga
      841 cttagtgatc cggtggttct gcgtggaagg gccatcgctc aacggataaa agctaccctg
      901 gggataacag gcttatctcc cccaagagtc cacatcgacg gggaggtttg gcacctcgat
      961 gtcggctcat cacatcctgg ggctgtagta ggtcccaagg gttgggctgt tcgcccatta
     1021 aagtggtacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcag
     1081 gcgtaggaaa tttgagaaga cctgtcctta gtacgagagg accgggatgg acg
//
LOCUS       DAKBNN010000390         1097 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000390 DAKBNN010000000
VERSION     DAKBNN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.398"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            18..170
                     /locus_tag="OUB02_003908"
     CDS             18..170
                     /locus_tag="OUB02_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCT8887586.1"
                     /translation="MFLSGTNIKTISERLGHADIKITMNSYSQFLEEMDREAFENLSK
                     ILFIIF"
     gene            241..1086
                     /locus_tag="OUB02_003909"
     CDS             241..1086
                     /locus_tag="OUB02_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCT8887587.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
BASE COUNT          485 a          107 c          141 g          364 t
ORIGIN      
        1 ataataatgc aactttaatg tttctttctg gaactaatat aaaaactatt tcagaaagat
       61 taggtcatgc agatataaaa ataactatga atagctactc tcagttttta gaagagatgg
      121 acagagaagc atttgaaaat ttgagcaaaa tactatttat tatattttaa agaagatgac
      181 ttattcaata tttgatgcta gaatataatt aataaaaatt taattatagg tgattgtatt
      241 atgaatatta ttaaaagcat aactttactc ttaacattat taattgtgat attaatgaca
      301 ggctgttccc caagagaaag tgctagtgaa gttgcaaaaa aatatattga taagatgaac
      361 tctggtgact atgaaacagc atatagttta ttaagtaaag atagtaaaaa aaaaattagt
      421 atagaaaatc ttgaagaatt tcaaaatatt ttagttagta caaaaagaat aattagttat
      481 aaaataggta aagaaaaaat cataaaaaaa tataaacatg atggaaaaga atataaaaat
      541 gttgttaaat tagaagaaac ttttaatata aaaaaccttc tttataaaaa agatgattct
      601 ctaaaagtaa atagatattt tgtagttgaa aataacaaat acaagctatt attgtatgaa
      661 agtttcaaaa aagaatttgg tattaatagt atggaccttg ctcaacttaa gcttaatgat
      721 tttggactaa aatactttaa agaagttgac cttatactaa aaaaagctat tgctattaat
      781 cctactgatt caaagcttta ttatgcccaa gcatgtaatt atatgaattt tgatggattt
      841 cctggttatt cagatacaac atataatgct ttagattcaa ttaataaatc tttaaagtat
      901 ttagataaaa atgattctga ctacaaaaaa aatgcaagta atatatataa cttaaaagga
      961 gttattctta tgtttcacaa aagatttgat gaagcacaac aatatttaaa taaagcctta
     1021 tactttaaca aagataatga agacccaaaa tataatctaa atacaataaa aaaacatcta
     1081 aactagatat ttaattt
//
LOCUS       DAKBNN010000391         1070 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000391 DAKBNN010000000
VERSION     DAKBNN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.399"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            243..626
                     /locus_tag="OUB02_003910"
     CDS             243..626
                     /locus_tag="OUB02_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887588.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKEYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
BASE COUNT          470 a           96 c          142 g          362 t
ORIGIN      
        1 agaagcaaga atactcaata aaacttaaag aaattaaaca atttctaaaa ataccttgaa
       61 aatagaacct tatttgccat ttataaatta tataaaaagg gattatataa agtcatatat
      121 gaaaaaacta tattatacta tcttttcttt ttggaataaa ctatacttta aatatagata
      181 tttaatttaa ataaatttta cagtattaat agtaaattat cttagaaagg aatgtgcttt
      241 atatgtctac agacaaagtt gttggaatca tagatgaaga aacggcagaa cttgcaggaa
      301 ttgaatatac tggtaaaatt tatgcttctt ctggtgttat aaaacatata aagaaaaaac
      361 atagatgtca actgagtaaa gatattttta atgatataat agacactata aaaatggtat
      421 taaagtcacc tgaatatata ggaagtcatc ctaaaaaacc tggaaaaagt gtggagttta
      481 taaaaaaaat tgatgattat attttagtag ctacagaatt agatacaaaa aaagaatatt
      541 tatatgtatc ttcaatgtat gctataaaag aagcaaaatt agaaagtaga gtaaacagtg
      601 gaagagtaaa acaatataat ggttaacata atattattaa ttttccctaa aatattgaaa
      661 aatattatta tataagttat aatattaata tacacgtaga tatttattag ttagatttga
      721 ggttggaaaa ggcacccaat acgctactat agagtagtta atagagatgc aggatacgcc
      781 gccctgccaa atctaattaa attaaaaaga gaggattaat tcctctctta ttgttttttt
      841 gagatactaa tttaatctat atagaaagat tgaaggtcat ttatactaaa agttttcagt
      901 tatatacatt aaactaatta aataagatat ttataatcta tatgtgatac atattatata
      961 ttaataaaag aaaaatatat agaaaatcct tataaaaatt taaattgtaa tgaataggaa
     1021 gtaattaaaa agaattttta ttgattttta aaaatttagt tagaaactat
//
LOCUS       DAKBNN010000392         1058 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000392 DAKBNN010000000
VERSION     DAKBNN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.400"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(138..629)
                     /locus_tag="OUB02_003911"
     CDS             complement(138..629)
                     /locus_tag="OUB02_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCT8887589.1"
                     /translation="MGMIGCYTKISEENVFKLQQSEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          398 a          154 c           95 g          411 t
ORIGIN      
        1 gacaaattta cctacagatt taaatttaac ttatatattt taaaaggcta atataatatg
       61 tattttcttc tatacaatca aaaggcttca tatctaagat atgtgatgac actaatattc
      121 acaattttta gtaattatca cattataaaa aatattacag ctagtccatc attagatgct
      181 tcttgtataa actttttaag ctcaattaga tttgcccata catattcaaa aaaatcatct
      241 tcactctcat cattcataac aggataaata ttattttcta acatctcatt aatattaaat
      301 ctttttctaa atgcttgttc ttccaaactg ttcattgctt ttgatagttt ttttacatca
      361 tttgctgtaa tcaacatgac aggaaattct ccatcaattt ccataagtat gtttccactt
      421 agtactaatt tgctaaatat attatcatca tctccaccaa aaggacaacc tgtcaaagta
      481 aagtgaatgg catgccatgc tttatcaata ttgatagtac tattttcgtt ggcatcttca
      541 aaaacaaaat cttgtaaatt ttcttctgat tgctgtaatt taaaaacatt ctcttcactt
      601 attttagtat aacatcctat catccccata attacattcc tcctaagtag cgttttttta
      661 attgtaactt ataattaaac ttagttacaa taaaatatat tgagaaaagt attatttcat
      721 cttaaattta aatacctata ttatcaacta aaattctgtg tttatagcgt aagtattatt
      781 attttttcct aaaaattaaa tgaatcagaa gttctatagg caatattgac tataaataag
      841 gactattact tttaaaaaaa atttaagaaa ttatactgta gaatcatgct ctacttatta
      901 gcttcaaata aaaaataaat catgttctca aattaaataa taaataacaa taaatttaaa
      961 ttttaaacta taagagtttc tgaagtgtta ataagcatac caataattat atgtgtaaga
     1021 atttgattta atagatataa ataattttta cattaatt
//
LOCUS       DAKBNN010000393         1050 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000393 DAKBNN010000000
VERSION     DAKBNN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.401"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            378..1028
                     /locus_tag="OUB02_003912"
     CDS             378..1028
                     /locus_tag="OUB02_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCT8887590.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
BASE COUNT          416 a           88 c          158 g          388 t
ORIGIN      
        1 tataattatt taaaactaaa tataaatgtt ggaattattt aggacttgaa attgtctaaa
       61 aataaatata agtgatgaag aggtgatgtg atgagattag atatggatag tatgttatat
      121 gtactaattt atatgacatt attggtatct atcattggaa gtatatttta tttttgtaaa
      181 gacctcttaa atcataaaat taagtatgtt ttaataagag acatatttgt aaatagaatt
      241 acatattctg aagaacggtt gcctaaacag tatatagttt ttcagaaata tgatatttgg
      301 aggtattgta gtttgtttta atttgaaatg aatctattat aaattctaaa gtttaaaaat
      361 aaaaaggaga gaataaaatg gatttcattt caagtatatt gcaaggatta ttaagtatat
      421 tatttaattt tacaggtgat tttggaattt ctattgtact tataacttta atagttaaat
      481 tagtacttat gccactatca ttaaaacaaa gattttctgt aaaaaaacag caagaattag
      541 ctgaaaaaat ggaacatata aaagaaaagt ataagaatga tactaaagaa ttggaaaagc
      601 aaatacaagt aagttctgta gaaagtatga agagtatgct gggatgtctt atagttttgg
      661 ttcaaatgcc agtagtatat gctttatatc atgtatgttt agatatgaca aaggaattta
      721 caacaattat aattccatgg attgctaatt taggtacatt tgataatttg tttatattac
      781 cttgtatata tacattaatt atgttagctt caaatttagt gaattatata ccatatttaa
      841 aaataaattc tcaagtaaag tttaacaaac aaatggctgt ggtgactacc ataacaagtc
      901 ttatactaac tgttagaact ccagtagcca taggtctgta ctttataaca agtgcaattt
      961 attcattaat tgaagatatt tgttttagaa tatattttaa tcgaaaaaat agaatagtga
     1021 caaaataata taattattat tagaaatatt
//
LOCUS       DAKBNN010000394         1032 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000394 DAKBNN010000000
VERSION     DAKBNN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.402"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>1032
                     /locus_tag="OUB02_003913"
     CDS             <1..>1032
                     /locus_tag="OUB02_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCT8887591.1"
                     /translation="HKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNL
                     KLATSIDISNDKVFEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAK
                     KTIEKDLKIEINEDEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAK
                     LENIFKEKLEILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRL
                     IEKYIETGNIYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKS
                     YFEREKIATTEIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKD
                     KILDYEVIFS"
BASE COUNT          458 a           87 c          166 g          321 t
ORIGIN      
        1 cataagtata atgaaaaaga atttatagag aattatatag aaaattatag gagcaaaaga
       61 agctcaattt tagaaaatga acaaaacaaa gaaattataa taaattcaat aaaaacattt
      121 tgtaaaaatt taaaattagc tacatcaata gatataagta atgataaggt atttgaagag
      181 tttttatata agcatattag tagtttatgt aaaaaaataa agttaggtat aaatcaatca
      241 cctataatgg ctcaagatat aaaaataaaa tatccatttg cttttgaact agctaatata
      301 gctaaaaaga caatagaaaa agatttgaag atagaaatta atgaagatga agttgcaaac
      361 atagccctac atataggagg agcagttgaa agagcatctt ataataataa agataaggtt
      421 ttcaaaacta taatagtttg tacttcaggc ataggcacat caatgttaat aaaagctaaa
      481 ttagagaata tatttaagga aaaattagaa atactaaaga ttattccttc atacttagtt
      541 gaatatataa aagttataga tgttgatttt gtaatatcta ctgttcctat tgaagtagga
      601 gatgtgccag taataaatat atctccaatt ttaagtgaga aagaaattag attaattgaa
      661 aaatatatag aaacaggaaa tatataccta gatttagata taaaaagcct gtttgatagt
      721 gaattgtttt ttactgattt ggtgtttaaa aggaaagaag aagttataga ttttatggca
      781 agtaaacttg tacaaaaggg ctatatagat gaggatatga aaaaatctta ctttgagaga
      841 gaaaaaatag caacaacaga aatagggaat atggtaacta taccacatgg agctattgga
      901 aaaatttttg ataataaaat agcaattggt atattaaaga agcctatttc ttgggaaata
      961 tcagatgttc gtttaataat aatgttagcc cttgataaag ataaaatatt agattatgaa
     1021 gtaatatttt ca
//
LOCUS       DAKBNN010000395          994 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000395 DAKBNN010000000
VERSION     DAKBNN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.403"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(29..796)
                     /locus_tag="OUB02_003914"
     CDS             complement(29..796)
                     /locus_tag="OUB02_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCT8887592.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
BASE COUNT          317 a          181 c           91 g          405 t
ORIGIN      
        1 agcctatttt tattatcaag attaaaaatc atctaaaaag ttcagatata ctttttgcag
       61 tttctataac tcttttaatc ttatactcaa agtccccttc tctcattctt atagttggac
      121 ctgacaaact tatggctgca atagctttat tatttttatc taagataggt gctccaatac
      181 aagttagtcc atgttctagt tcttcatcat ctatagcata tccttgttct tttatttttg
      241 ctaaaaaaag catcatatca tcccaattat caattgtatg ttctgtatac ttgtgtattg
      301 gtgtttttct atatttttca aaatctatag aatcattaaa tgccataaga catttaccaa
      361 ctgctgaaca ataacattca ctacttgaac caacggatgg atttactgaa agtaattgat
      421 ttgaaccata ctctttatgt attatgatac ttcttggaga atcttgagct ctttcttcta
      481 atatagatac attaactact tcgttaaatt cttgatttaa ttttttcgta tatggtttta
      541 ctatttcagt caatgacatt ttttctccca caaccatacc tattgcatat aaatttattc
      601 ctagccaata cttaccattc tctgcatttt gtataacaaa acctttgttt tcaagagtaa
      661 ccaaagttct atgtacagta cttttatata ctcccatagc cttgcttatt tcagttattc
      721 ccatttctct cttttcatga tataacagca aaattatgtc tagggctcta tctaatgcat
      781 ttattatttc tcccatatac aaaaactcct cctgcaaata ttaagtgcta ctcccacact
      841 ctactaaata ttatatatta aaattacaaa attatctatt tatcttaatt atattttata
      901 aaacaaaaat attatactta atttgtaaaa taaaaacaaa atactcacta tgtccttatt
      961 attcttactt ttaatagaat tattctgtta tatt
//
LOCUS       DAKBNN010000396          992 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000396 DAKBNN010000000
VERSION     DAKBNN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.404"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            30..947
                     /locus_tag="OUB02_003915"
     CDS             30..947
                     /locus_tag="OUB02_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887593.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSELDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT          352 a          120 c          167 g          353 t
ORIGIN      
        1 taaattaaaa tataaaagga gtgtatttag tggaaaataa taaatgtaga gaggacttta
       61 gatttacaca agaatatgag gaagattatc caaatacaaa tgaaagatac tatgaaaatt
      121 atcaagtagc tgatagatac tataattatc caaataaata taaagaacct aaaataaaac
      181 aatgttgttg taaaaaaagt atgagagagg ccttagaact tctaagatat gatgctctaa
      241 gaccttttgt aaactttaat caatttgctt ttatctcaga tttctttata gtaggtgcta
      301 atttggtagg tatagatctt tcagctcctc caaaagataa tttatctgaa cttgatggta
      361 cttttgaaag attttctgct tgtaactgtg atttaataga tatagctggt agagtatctt
      421 atcctattcc agtcccttta actcttgagg gattaattaa tactatagga actataccag
      481 gagtagctga attaattgca cttattgatg cagttattcc tcctacgata gaccttgggg
      541 ctatattaga tgcaatactt gctgctataa ttgattttat acttgctgca tctactccat
      601 tagcaaacgt agatttagca tcattgtgta atcttaaagc tgttgcattt gatattacac
      661 ctgcagatta tgaagatttc atagcatctt taggttacta tcttgataaa aaacattaca
      721 aagaatgtaa ttgtaactgc gattgtgatg attgctgttg taataaaggt atcctagata
      781 atctttatat gtcaaatata aataatcaag ttactgtagt agctggtagt ttggttctaa
      841 ctggtgttga agttctaggt aagaaaaatg atgttatagt acttggaaat tctaatgatt
      901 caagaatata ctttgtatgt gtagattcta tagattatat tgcataagaa tgattttaaa
      961 gagttatatc taaatataaa atagaatcta tt
//
LOCUS       DAKBNN010000397          978 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000397 DAKBNN010000000
VERSION     DAKBNN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.405"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(152..685)
                     /gene="pyrR"
                     /locus_tag="OUB02_003916"
     CDS             complement(152..685)
                     /gene="pyrR"
                     /locus_tag="OUB02_003916"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCT8887594.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
BASE COUNT          329 a          176 c           78 g          395 t
ORIGIN      
        1 acgacatcaa aaactcaaat aaaggagaat aaaaactaca ttaagactca catccataaa
       61 attataatat ttattttgtg caatctatgt aagctttatc ctcctccctg actattcaat
      121 gctaagcttt ttatataata cttaaaaata attactcttt gattgtaact gaatcttctc
      181 catcaatttc taataaactg acagatataa tttcatgtct agaagtaggt acattttttc
      241 ctacataatc tgctcttata ggcaactctc tatgccctct gtccactagc actgctaatt
      301 gtattgattt aggtcttcct atatctataa tagcatctag tgaagctctc acagttcttc
      361 cagtgtataa aacatcatca actaaaacta ctgtcttatc atttacatta aaatctaaat
      421 aagttccttt aactacaggt tcaacatgaa ttttttccaa atcatctcta tacaaagtta
      481 tatcaatatc tcctgtatct acttttgttc cttctatttg ttctattttc ttagaaattc
      541 tatttgcaat tggcacacct ctagtcttta ttccaactaa aactagattt tctacacctt
      601 tatttctttc aattatttca tgacttattc tagtaattgc tcttgctatt gccttttcat
      661 ccattaattg agctttttct accatatcat aattattgtc aaagatataa tatatctatt
      721 tgacaaatct actcctttct tttaattttt tataaattta tttttattta aaatttcaat
      781 ttaaacaaca aaaaaacttc tcatcacaag acaagaagct cttatataaa aaatatacag
      841 tgcaatttag caattgtctt acgatatctc tgtatcgttt aaagataatt ttttaatcta
      901 tcaaatcaaa ttatatattt atatttcaaa tttttcaagc ttttttaaac aaaatacttt
      961 aatataagat attttatt
//
LOCUS       DAKBNN010000398          940 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000398 DAKBNN010000000
VERSION     DAKBNN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.406"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..938)
                     /gene="bclA1"
                     /locus_tag="OUB02_003917"
     CDS             complement(<1..938)
                     /gene="bclA1"
                     /locus_tag="OUB02_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HCT8887595.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGTTGVTGPTGNTGVTGNTGV
                     TGNTGVTGSIGPTGATGPTGVTGEIGPTGVTGPTGVT"
BASE COUNT          232 a          206 c          126 g          376 t
ORIGIN      
        1 gtcactcctg ttgggcctgt tactcccgtt ggtcctattt ctcctgtcac tcctgttggg
       61 cctgttgctc ccgttggtcc tatacttcct gtcactcccg tatttcctgt cactcccgta
      121 tttcctgtca ctcccgtatt tcctgtcgga cctgttactc ctgttgttcc tatacttcct
      181 gtcgctcctg tacttccggt tgggcctgtt actcccgttg gccctatact tcctgttgct
      241 cctgtatttc cggttgggcc tgttggtcct atgcttcctg tcgctcctgt acttccggtt
      301 gggcctgtta ctcccgttgg tcctgtactt cctgtcgctc ctcttggtcc cattggacct
      361 gtaattctaa ttggaagaca ctcacattta ggataacaat tatctataat tctatcttca
      421 aatgttacaa gtatatacgg tttgttacaa cctctactag atgtaaattc aagaatctga
      481 taagggtaat ttaaagataa agctaaccca taattaggat atttattcat tgcccatgct
      541 tttatagttc ctgtaatatt taaacaaata tagttggatg tatcacttat cccaactggt
      601 aaatatcccc taatattttc catagacact ctatcattcc atgttatagt tcgtaaatta
      661 taatattcac ttattctttt aatttcaata tctacttttt ctttaaaaat atgattctta
      721 tttgaatcaa tatgaataaa taactctgct tttaatatac ttttgtcttt taatatatct
      781 ggtattctca ctttaaaaaa agtaatcaac ttttctttta caaaactatt tgaacattta
      841 cttcctatta agcaataatc taaattacta aagtttttat ctggatattt tttagatata
      901 aaagtatcat catttaaata aagtataata tttctcattt
//
LOCUS       DAKBNN010000399          928 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000399 DAKBNN010000000
VERSION     DAKBNN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.407"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            9..>928
                     /locus_tag="OUB02_003918"
     CDS             9..>928
                     /locus_tag="OUB02_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCT8887596.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSE"
BASE COUNT          316 a           84 c          116 g          412 t
ORIGIN      
        1 aaaattttat gatttttaca attatttttt ataaaaaagg gaggttatgt ttgttgaatt
       61 tacaatcaaa atgggtaaac tttttaaagc caacatatgt cagtgatgaa ataaaaagaa
      121 aaaaactaca agttataata tttttttcta taacttatgg tttatcttat tcactaggtt
      181 tattagcaat atgttttaat catttaatag attcagaaat tttagctcaa tttatgatga
      241 tattgccatt atcttcagtt tctattgcaa aattttacac agagggaaga aataatgata
      301 aatataattt ttattcatca gtaatattat tttttattat ttactttttt atatttatta
      361 ttgaatcatt aaagttaata agtattaatc aatttcaatt aatgtatctt atgttaattt
      421 taataagtag tttatgtgtt attgtttatt cttataaaat aacaagttta tatattttta
      481 aaaattttaa gataggtatt ttgctatttt tttacttttt attttctcaa attatttttg
      541 gattaataat aagtgataat caatttgatt ataagaattt cttgaattat attcttcttc
      601 ctataatatc attagtatac atttatcctt ttttatgtga agagtatggt tggagatctt
      661 ttttgcaaag tattttattt gatagatttg gcaaaaaaat aggtattgta atggtaggca
      721 cttgttggag tttatggcac ttacctttac aatttacact atatagtcct caaactccaa
      781 taattggagc tatagcccat ttgatatatg gtattggact ttctatattt ttaggttatg
      841 tttatatgaa aactaagaac atatggtttt gctctataat gcatgttcta attaatagct
      901 taggattaat tttcaatgat tctgaaat
//
LOCUS       DAKBNN010000400          893 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000400 DAKBNN010000000
VERSION     DAKBNN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.408"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..614
                     /gene="srtB"
                     /locus_tag="OUB02_003919"
     CDS             <1..614
                     /gene="srtB"
                     /locus_tag="OUB02_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HCT8887597.1"
                     /translation="NIYSKLTKYNHDTKISSELQKKEYKKEDLSKINSDFKFWLSVEN
                     TNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYRNKSIDDKNIIIYGHNMKNKTMF
                     NNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYIVESDYDYLKTNFNNESDYQNYI
                     NDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARMVIHGRLI"
     gene            complement(774..848)
                     /locus_tag="OUB02_003920"
     tRNA            complement(774..848)
                     /locus_tag="OUB02_003920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Thr,seq:tgt)
BASE COUNT          383 a          118 c           99 g          293 t
ORIGIN      
        1 ttaatatata ctcaaaactt accaaataca atcatgatac taaaatatcc tcagaacttc
       61 aaaaaaaaga atataaaaaa gaggatttat caaagattaa cagtgatttt aaattttggc
      121 tttctgttga gaatacaaat ataaattatc cagttgtaca atctaaagat aattcttact
      181 atcttgacaa agatttttat aaaaaagatt ctatttctgg tacactgttt atggattata
      241 gaaataaatc tattgatgac aaaaatatca taatatatgg acataatatg aaaaataaaa
      301 ctatgttcaa caacctaaat aagtttaaag atgctgactt ctttaaaaaa aataataaaa
      361 taaaaataac cttgaatgga aaagaatttt tatatgatgt tttttctgct tatattgtag
      421 aatctgatta tgattatcta aaaacaaatt ttaacaatga atctgattat caaaattaca
      481 taaatgatat tacttctaaa tcattgtata aatctcctat taaggtaaat agcaatgata
      541 aaatagttac actttctaca tgtacatatg aatttgatga tgccagaatg gtgattcatg
      601 gtagattgat ttaaaactca cagacacagt ttaaaacaat aattttaaac tgtgtcatct
      661 tttataccta taatttataa attagaccac taaaagtatt taatacaaaa taacaaatac
      721 atcaaacaat aaaaaacaat aaaaaaagac tatgctaaaa acatagtctt tactggagct
      781 ggtgatagga atcgaaccta caacctgctg attacaagtc agctgctcta ccgttgagcc
      841 acaccagcac acctatttta ttgtaaatta gtaaaactaa tttttaaaag tta
//
LOCUS       DAKBNN010000401          862 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000401 DAKBNN010000000
VERSION     DAKBNN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.409"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            71..730
                     /gene="cprR"
                     /locus_tag="OUB02_003921"
     CDS             71..730
                     /gene="cprR"
                     /locus_tag="OUB02_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HCT8887598.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT          348 a           54 c          166 g          294 t
ORIGIN      
        1 tgtgaatttt aaagatatta ttaaatatat aaattagaat gctattagat ggataaatgg
       61 aggagttatt atgtctaaga tattagtagt agatgatgag ttagatatgt taaagttgat
      121 tgaaaatgtg ttgaaaagag atggacatga agtaaaggta attagtgatg tagaaaaggc
      181 tatgaacatg gattttaatt attttaatct aataatatta gatgttatga tgcctaagat
      241 ggatggtttt gaagtatgta aaactataag aaataaggtt gattgtccaa tactgttttt
      301 gacagcaaaa aatatggaaa gtgatattat gtacggtctt ggtataggtg cagatgacta
      361 tataaccaaa ccttttggtg taggagaatt aagggctagg ataactgctc atttaaggcg
      421 agaaagtaga gaaaaaaaga atttattaac tatctcaaat gttcaattta actttttagg
      481 caaagaagtt tcaattgatg gtaaaaaaat taattttact aaaagtgaat atctaatatg
      541 tgaatttcta gcaaaaaata agggacaagt attttcaaaa gaaagaatat atgagtcggt
      601 atatggtttt gatggagaaa gtgatataag tactatcaca gagcatataa aaaatatacg
      661 aagcaaatta aaacaatatg atatttatac aatagaaact gtatggggga tagggtataa
      721 atgggtgtaa taagtataag tgttgtttag tgaaaatatt atatatatct acaaaatgtt
      781 tgtaaaatat taatctatat attaaaattt aatttataga ttaatatttt ttgtaaatga
      841 caaaattgta aggtattttg tt
//
LOCUS       DAKBNN010000402          858 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000402 DAKBNN010000000
VERSION     DAKBNN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.410"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            63..761
                     /locus_tag="OUB02_003922"
     CDS             63..761
                     /locus_tag="OUB02_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCT8887599.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELISIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPAKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          329 a          109 c          131 g          289 t
ORIGIN      
        1 cattatttta tagtttgatt aattcaacaa tcactaatat atcaaactga aaggagattt
       61 ttatggaaaa aaaatatttc gaaattcatg gagttccttc aatcctttgg ggaaatcctt
      121 caagtaaatt atatatttac atacatggac aatgtggcaa taaagaggag gctgaagctt
      181 ttgctaatat tgcagttcca catgagtgtc aagttttaag tgttgatttg cctgaacatg
      241 gagaacgtaa aagagaaaaa aattcattta acccatggca tgttgttcca gaattaatat
      301 ctattatgga atatgcaaag tgccactgga atcaaatttc gttatatgca aatagcattg
      361 gagcatggtt tagtatgtta agctttgaaa aagagaactt aagagagtgt atttttgtat
      421 ctccaattct agatatgcaa cagctcattt caaatatgat gctttgggca aatgtatcag
      481 aagaacagct taaacatgaa ctaattatcc caacttcatt tgggcatact ctttcatggg
      541 aatatctaca atatgtaaaa caacatccta tcactcaatg gaatgtacca gcaaaaatat
      601 tatatggtaa acatgatgaa cttactgaaa tcggtgttgt agagaaattt gtagaaaagt
      661 ttaattgcaa ccttactatt atagaaaatg gcaaacattg gtttaacaca ccacaagaat
      721 taaattcatt atatgaatgg ttaaatactt tagttaaata aaaaataatt atattattaa
      781 tatgattaaa ttatttataa atgttattta attaatttta ttttagcatt attaaaataa
      841 ctacataaag aataaatt
//
LOCUS       DAKBNN010000403          854 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000403 DAKBNN010000000
VERSION     DAKBNN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.411"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(193..660)
                     /locus_tag="OUB02_003923"
     CDS             complement(193..660)
                     /locus_tag="OUB02_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCT8887600.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRHFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          329 a          151 c           54 g          320 t
ORIGIN      
        1 cttttatata ttttttctaa atttaaactt tagtataaat atataagaaa tatcataata
       61 tttttaaaaa ctcaaataaa gctaaacaag tattatattc tttattttag aaaaataaaa
      121 actgtaagca aatttgtttt cacacaagtt tacttacagc cttcacctca attttacttt
      181 aatataatcc atctattcta aaactgattc aatatattcg acagttacat ccattctttt
      241 agctacctca tctattgaaa gaccactatc taaaaaatgt ctacacacag atgacatagt
      301 ttctccaacc tcaataatat gcccatctaa atcataaaat ctaattgctc tttgacccca
      361 cctatgctct atagctagat gaacataatc tatatctttc attgtactca atctttcaac
      421 aaaagtatca aaattatctt cctcaaaata taactcagca tcatttccat tgaacttaac
      481 ttcactgtca ctcttattaa taaattctaa ccaaagcttt ttttcttgta atgatatatt
      541 accttctagt acaacatttt ctccaaaatc acagattacc tttaacccca aaacatcata
      601 ataaaacttt tttgatttct caatatcttc aactactact aaaacattta aaaacttcat
      661 attacctcct taatatacac atactttatt tataatatat aattagaatt aaactataaa
      721 ggtttcaata aggtctagta taaagaaaaa aattataagt cttattgaaa tagttaagtt
      781 aattttatca tataatataa attttaatct tgtaaaaaac agacatgttc atacttttag
      841 gtttaaatct atat
//
LOCUS       DAKBNN010000404          832 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000404 DAKBNN010000000
VERSION     DAKBNN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.412"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(110..817)
                     /locus_tag="OUB02_003924"
     CDS             complement(110..817)
                     /locus_tag="OUB02_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887601.1"
                     /translation="MISIAICDDESYMLNHLKENIELYMKQKNLLYNIELFNKVEDLL
                     CNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAINYL
                     IKPLSNEKLYKTLDKIVTKLEDNESNYITIQKGYSYWKLKLADILYCEVIKRKIYVHL
                     KKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVSRLR
                     QQIFSKVMLEFMKNRRI"
BASE COUNT          307 a          136 c           69 g          320 t
ORIGIN      
        1 actgattaga aaatagtatt gcactattac cattaaaatt ctatatacaa taaaagaaaa
       61 taaaaaactg tactttaaaa ataaatcgga agaaaataaa ttaatcatct tatatcctcc
      121 tatttttcat gaactcaagc ataacttttg aaaatatttg ttgacgtagt ctagaaacag
      181 gtattctatc tccactttcc ataactgcat taccattttc atatccatat acatattgta
      241 aattgattat ataactacga tgacacctaa aaaaatttgt tggtaaactt ctctctaaat
      301 tttcaatagt ctcataataa tctacagtct ctttctttaa atgaacataa atctttcttt
      361 ttataacttc acaatataaa atatcagcaa gttttaattt ccaatatgaa taaccttttt
      421 gtattgtgat ataattactt tcattatcct cgagttttgt aactatttta tccaaagttt
      481 tatatagttt ctcattagat agaggtttta tcaaataatt tatagcatct acttcaaaag
      541 catcaaatac aaattcattt aaaactgtaa cgaaaattat aaagcactca ttaccatatg
      601 accttaattt tctagctgcc tgcattccgt taatctcttc catctgtata tctaaaaata
      661 caatattaaa cttttcatga ttgcatagta aatcttctac tttattaaaa agctctatat
      721 tatataataa attcttctgc ttcatgtata attctatatt ttcctttaaa tgatttagca
      781 tataggattc atcatcacaa atggcaattg aaatcaatat atacaccttc tt
//
LOCUS       DAKBNN010000405          813 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000405 DAKBNN010000000
VERSION     DAKBNN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.413"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>813
                     /locus_tag="OUB02_003925"
     CDS             <1..>813
                     /locus_tag="OUB02_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887602.1"
                     /translation="ISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGASIVAF
                     SLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLRIMGK
                     SNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQMIKSL
                     GINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKK
                     FTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLN"
BASE COUNT          244 a          120 c          131 g          318 t
ORIGIN      
        1 ttatatctgt aatgacgcaa agtttaattt tatcgattat ggcactgggg gtttatataa
       61 cttacaaaat cttagatttt ccagatatgt ctgctgatgg tagctacaca atgggtgctt
      121 ctattgtagc attttctctt actaatggta tttctcctgt agtagcaact ttaatggcta
      181 tcttatgtgg ttgtactgct ggtcttgtta cagggattct tcacattaag tttaagatat
      241 ctaatttact ttctggtatt ttagttatgg gaatgctata ctctatcaat ttaagaataa
      301 tgggaaaatc aaatattcct ctatttagct ttaaacactt attcaatggg gaaatatctc
      361 ctatagtttt agccttagct tttgtattca tctgtaaagt ccttttagat ttattcctaa
      421 aaacaggtct tggatacact ttaaaaggtg ttggtgataa ctctcaaatg attaaatccc
      481 taggtataaa tataggttct ataaaaatac taggacttat gatttcaaat ggacttatag
      541 ctttatctgg tagtcttatg gctcagttct tgggattctc agatgtaaat atgggtatag
      601 gaactttagt tcttggaatt gcctctatca taattggtat tacactattt aagaaattta
      661 cttttatcaa agatactact gcaataattg ttggctcttt tatataccaa ttcactatat
      721 actttgcaat gagtttagga atgttatcaa ctgacttaaa actaataact gccatagtta
      781 taatagcttt cctagctact ggtaacttaa ata
//
LOCUS       DAKBNN010000406          793 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000406 DAKBNN010000000
VERSION     DAKBNN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.414"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>793
                     /locus_tag="OUB02_003926"
     CDS             <1..>793
                     /locus_tag="OUB02_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8887603.1"
                     /translation="YHKLLVDQKVGYSVGNPIVFNADDDNLTKLLNDLLGEEFDDTIT
                     ELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWDSKRQRELVAFIRFYYIEDI
                     DGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMTDIQEGHFRINNKEQGWGKV
                     PFIPFKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEIQEVIYVLKEYPGTSLQEFI
                     DNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKNIIIFGQG"
BASE COUNT          326 a           81 c          144 g          242 t
ORIGIN      
        1 actaccataa gcttttagtt gaccaaaagg taggctatag tgtcggaaat cccatagtat
       61 ttaatgcaga tgatgataat ctcactaagc ttttaaatga cttactagga gaagaatttg
      121 acgatacaat aacagaactc tacctcaatg ctagtaacaa aggtattgaa tggttacatc
      181 catatattaa tagaaaaggt gagtttaaat atgtaataat acctgctgaa gaagcaattc
      241 ctatttggga tagtaaaaga cagagggaat tagttgcatt tattaggttt tattatattg
      301 aagatataga tggaaataaa ataaaaagag ttgagtacta cacagaaaat gacgtaactt
      361 actttattga aagaggtaat agttttattc aagaattttt atatgatgaa tatggaaaaa
      421 tgactgatat acaagaaggt cattttagaa taaataacaa agaacaggga tggggtaaag
      481 ttccatttat accttttaaa aataatgaaa agtgcgtatc agatttaaat ttctataaat
      541 cattaataga tatatatgac aataatattt ctacactagc agataactta gatgaaatac
      601 aagaggttat ttatgtatta aaagaatatc caggaacaag tctacaagag tttatagata
      661 atataagata ctataaatca attaaagtag atggtggagg tggagttgat aaactagaga
      721 taaatatacc agttgaagct aaaaaggagc ttcttgatag attggaaaag aatataatta
      781 tctttggtca agg
//
LOCUS       DAKBNN010000407          769 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000407 DAKBNN010000000
VERSION     DAKBNN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.415"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..174
                     /locus_tag="OUB02_003927"
     CDS             <1..174
                     /locus_tag="OUB02_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCT8887604.1"
                     /translation="QPIGARQIMNYYMKHSNSGDVKIYSKKEMLLLLSKDFHEIEWKS
                     FGTRSCLIIAYNN"
     gene            198..758
                     /locus_tag="OUB02_003928"
     CDS             198..758
                     /locus_tag="OUB02_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8887605.1"
                     /translation="MRISKEPEERKQEILETAIKLFSVNGFEKTSISDIAKEIGIAQG
                     LCYRYFPSKDVLFQSAINEYSNILVANMTKNINIKKDSLKDILRKMILFAEQEDDTYY
                     PVFHNNQNKNFHNLLTLNVCEKLFPIVSEIIERANYTNEIQVNDVEMYASFCIYGQLG
                     IILNTDISIKEKSSRIKAFFRDLFRL"
BASE COUNT          321 a           96 c          119 g          233 t
ORIGIN      
        1 caaccaatcg gagcaagaca gataatgaat tactatatga aacacagcaa tagtggagat
       61 gtaaagattt attcaaaaaa ggaaatgttg ttattattat caaaagactt ccacgaaatt
      121 gaatggaaat cttttggcac aagatcttgt ttaattatag catacaataa ttaaaccgca
      181 agaaaggaaa gaaaacaatg cgtatttcaa aagaaccaga ggaacgtaaa caagaaattt
      241 tagaaacagc cattaaatta tttagtgtaa atgggtttga aaaaacttca atatcagaca
      301 tagcaaaaga aattggaata gcacaaggat tatgttatag atatttccca tcaaaagatg
      361 tactttttca gtctgctatc aatgaatatt caaatatttt agtcgccaat atgacaaaaa
      421 atataaatat aaaaaaggat agtttaaaag atattttgcg gaagatgata ctttttgcag
      481 aacaagaaga tgatacatat tatccagttt ttcataataa ccaaaataaa aatttccata
      541 atttattaac attgaatgta tgtgaaaaac tttttcctat tgtatctgaa attattgaaa
      601 gagcaaatta cacaaatgaa atacaagtca atgatgttga aatgtatgcc agtttttgca
      661 tatatggaca actaggaatt attttaaata ccgatatatc aattaaagaa aagagttcac
      721 ggataaaggc attttttagg gacttgttta ggctttaaat cttcccctg
//
LOCUS       DAKBNN010000408          762 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000408 DAKBNN010000000
VERSION     DAKBNN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.416"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>762
                     /locus_tag="OUB02_003929"
     CDS             <1..>762
                     /locus_tag="OUB02_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887606.1"
                     /translation="LFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKSNSSVL
                     AVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLLYKYKK
                     DNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNNNYEEL
                     TSKESVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEINSQATF
                     DLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELD"
BASE COUNT          337 a           62 c          110 g          253 t
ORIGIN      
        1 tttatttttt tcatcaacta tgtatatgat tattgatgct gttaagaaga gaaaaagttt
       61 ttattataag ggacagaatt taattgcatt ttctgaactt ggatttaaat taaagagcaa
      121 tagtagtgta ttggctgtaa tagcaatatt aatagctaca agtgtaacta tgctagggtt
      181 taccatatca ctttattacg atatagatag gaacataagt gaaaattata aatacagtta
      241 taccataaat ttaggaaatt cttatgtaaa taacgaaatt gacaaattat tatataaata
      301 taaaaaagat aataagataa tctttgataa aacaatagaa cttatagaca aagatataaa
      361 tattgaaatg tattataaaa atggaaatag ttatatagaa gatagaacgt ctgtagatat
      421 tattagagaa agtgatttta aaaaattaag acaatatcag aataacaatt atgaagaatt
      481 aacttcaaag gaatctgtgt actatgtttc tgattcatac aaaaaaatat tttttaaaga
      541 ctttgagatt gaaaaaataa acttgcatat taaagataca aatgagtatg atataaatct
      601 gtttaaagta caaaaaagca tcttggagcc agaaataaat tcacaagcta cgtttgattt
      661 aattgtagta aaagatgacg tatttaatca gttaaaatta catggaaatt ctagattaat
      721 aagattaatt gatataaaaa atgaaaaaaa agaactggat tt
//
LOCUS       DAKBNN010000409          753 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000409 DAKBNN010000000
VERSION     DAKBNN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.417"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            390..695
                     /locus_tag="OUB02_003930"
     CDS             390..695
                     /locus_tag="OUB02_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887607.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          330 a           68 c           99 g          256 t
ORIGIN      
        1 atagatgaag aactaaactt atagattaaa aaataaatga cttatagatg aaaaatcaag
       61 ctcatagatt aaagaataaa taacttatta gatgaaaaaa caagcttata gataagaagt
      121 ccatgcttat aagccagaaa atgaatactt taatagatta aaaaatacat atttgtgaat
      181 tatagaatga atgactccat aaactagaaa atttataata atatctatat gaaaattaga
      241 attattttat cacaaaaaaa ttaagttaaa taaatatgaa attacttcca aatatacttc
      301 caaaaacatg taatcgcata tataatataa ttataaaaat aataaatatt tttatagtag
      361 tagttaatta ttaataagga ggggtcaaaa tgcctagcat gattatggaa gttgtaggag
      421 tactatttat agctattgga ttaatcttga cattcattga aataaaagga aatttccctt
      481 ctttttatgg ttttggtcaa aattctatag ggaaaaatgc tgtaggtatt atagcagcag
      541 gaattgcttt aattttcttt agaaatggca tagatagcat agattctata gctacttatg
      601 gtgtattttt agcaatagaa cttgttgtaa tagttgcata tttcttaata agaaaaaaat
      661 ttactaaaga agataaacat ttaagaacta attaactaaa ttatctacta taaaattgga
      721 aaattaaaaa caaataaaag tttgttttta att
//
LOCUS       DAKBNN010000410          739 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000410 DAKBNN010000000
VERSION     DAKBNN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.418"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            202..438
                     /locus_tag="OUB02_003931"
     CDS             202..438
                     /locus_tag="OUB02_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887608.1"
                     /translation="MNNMENKKNFLRKIVKLRSIFLSIFGVFLFITATTGIQTVDRYV
                     PTWLYITLGVSATISLVLYVISFILKINIKDSKM"
BASE COUNT          327 a           58 c           75 g          279 t
ORIGIN      
        1 tgttaaatct tttttattta tgtgaacttt taacagaatt taatatttta atttttaaat
       61 tttatatata agataaaaaa ataaaacaaa acagtagcta ggctttaaat aaaatattaa
      121 atactataat ttatagatta aatctgatat tattatatac atctggaagt tgttgctatt
      181 atttaaaaag gtgggatgga tatgaataac atggaaaata agaaaaattt tttaagaaaa
      241 atagtaaaat taagaagcat atttctaagc atatttggtg tgtttttatt tattacagca
      301 actacaggta ttcaaactgt tgatagatat gttccaacat ggttatatat aacattggga
      361 gtgtcagcaa ctatatcatt agttttatac gttatttcct ttattttaaa aataaatata
      421 aaagatagta aaatgtagta cttcataaac ttccagtaaa agaataattt tgataaatca
      481 aaattattct tttttaatat caaaataaat aataaaataa caaaataaat agaaaatata
      541 actaaaaaat tatcaaagtt taagatgata attttatatt tgataaactt atgtaataca
      601 ttgtaaatta acttctcaaa atttataatt cttagcataa aattataatt taatattaaa
      661 attttacaaa aaagtaattt attaaatgac taggataatc aataaaactt tataatttcc
      721 acaaaagtta tgatgaaat
//
LOCUS       DAKBNN010000411          729 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000411 DAKBNN010000000
VERSION     DAKBNN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.419"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..649
                     /locus_tag="OUB02_003932"
     CDS             <1..649
                     /locus_tag="OUB02_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCT8887609.1"
                     /translation="FITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIIS
                     SLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVAT
                     VSNLDNMNVLLSGSLYRYEAIFLGKLMLGILLYVYKASFKNFKISKKEIYICIPMIAN
                     LLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKIR"
BASE COUNT          267 a           62 c           88 g          312 t
ORIGIN      
        1 atttataaca attgtttctt ctattataga aatatggtca tgtaaaaaag tttttgatta
       61 tactagtcag tcaaagaccg acttaaaaaa actaaatatg tattttctct tttctgtttt
      121 tctcattatt tcttctttat atataaatat aggagctaat agtagaatat tgatatgtat
      181 atttgtaatg ttcgttttct ataagtctaa ctataaggtt aatatatata aatgtctggt
      241 aatctcattt ttttattggt taatattaat aggtataaat ggattaagta tgttattagt
      301 agcaacagta agcaatttgg ataatatgaa tgttttactt agtggcagtt tatatagata
      361 tgaagcaata ttcttgggaa agctaatgtt aggtatatta ctttatgttt ataaggcttc
      421 ttttaaaaat tttaaaataa gtaaaaaaga gatatacata tgtattccta tgatagcaaa
      481 tttattgtca ttttttgtac tttatgatta tattattaat cttgtaaaaa tctctcctga
      541 aagaagaact gcttttttag taatagtttt actattaata ttatctaaca tttcaatctt
      601 tgtattaata catataatac ttaagattaa aattaggaaa ataagataaa ttttaatacg
      661 attcatttaa attaaaagtt aataattact tttttatgta taattttaca tgaatttcta
      721 taaaagtta
//
LOCUS       DAKBNN010000412          710 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000412 DAKBNN010000000
VERSION     DAKBNN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.420"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            73..675
                     /locus_tag="OUB02_003933"
     CDS             73..675
                     /locus_tag="OUB02_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HCT8887610.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIKPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT          301 a           58 c          131 g          220 t
ORIGIN      
        1 agtataaaga atatctactt gattaataca atatagctgg caaatataca attgataagg
       61 agtaatttta taatggataa tttaattagg atactgatat tagctgttat tggtggattt
      121 ataggttatg ttacgaatgt agttgctata agactgatat ttagaccaat aaagcctatt
      181 aaaataccta ttttaaatat agaaatagta ggtcttatac caaagagaag agcagaaata
      241 gcagcaaatg taggagaaat aattcaagaa gaatttcttt ctatggacga aatacttgca
      301 aatatcatta ctgacgaaga taaggaagaa gttgtaagat acataaaagc aagggtaaaa
      361 attataatac atgagaaagt ttcatttatc ccgagtggta taaaaaacat gatacaagat
      421 tatctaggag agataattga gtctgaagtt aagcaaagta tagatgaatt gagtaaaaat
      481 ataataaata aagcaaatga acgtatagat atacaaaaaa tggtcgaaga taagattaat
      541 gaactagatt tatatgaatt ggaagagata ataataagaa tagctaagaa agaattaaaa
      601 catatagagt ttttggggct tgtcttaggg tttttaatag gtatagctca agggttaatt
      661 actatgttta tttaaatata tgagaagtag catttattta agtactaatt
//
LOCUS       DAKBNN010000413          682 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000413 DAKBNN010000000
VERSION     DAKBNN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.421"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..640)
                     /locus_tag="OUB02_003934"
     CDS             complement(<1..640)
                     /locus_tag="OUB02_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCT8887611.1"
                     /translation="MNKNIDFVNGNTKKCLLEMTVPMIIAMFLNMAYNLVDSLWIGNL
                     LGEQAYAALTNSTPIILILTSIGMGATNGLAILLSQVIGAKNEQKKKNIIVTSFFVSI
                     ILSVFMTIVLEIFLEPILIVLNTPTEIFDMAKNYLSIYALGYVAVYLYLYFTAILRSF
                     GNTMFQAVAMLVSTLLNAILDPIFINIIGFHGAAIATLISQIICLAFMCIYIY"
BASE COUNT          258 a          114 c           87 g          223 t
ORIGIN      
        1 gataaatata aatgcacata aacgccaaac aaataatctg tgatattaag gttgcgattg
       61 ccgctccgtg aaatccaatt atgttaatga aaataggatc taaaatagca ttcaataaag
      121 tagacacaag cattgcaact gcttgaaaca tagtattgcc aaaacttctt aaaatggctg
      181 taaagtataa atacaaataa actgctacat atcccaaagc atatatagat aagtaatttt
      241 ttgccatatc aaaaatttca gtcggtgtat ttaaaactat caaaataggc tctaaaaaaa
      301 tctccaacac aattgtcata aacactgaaa gaattataga tacaaaaaaa gaagtaacta
      361 taatattctt tttcttttgc tcatttttcg caccaattac ctgtgataat agaatggcta
      421 acccgtttgt tgctcccata ccaattgaag ttaagattaa aataataggt gtcgagtttg
      481 ttaacgctgc gtatgcctgc tcacctaaca aatttccaat ccaaagactg tctacgagat
      541 tatatgccat atttaaaaac atagctatta tcataggaac agtcatttca agtaaacatt
      601 tttttgtgtt tccgtttaca aaatcaatat ttttgttcat tttaattcct cctattgaat
      661 gaaaattatt catttaattt at
//
LOCUS       DAKBNN010000414          664 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000414 DAKBNN010000000
VERSION     DAKBNN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.422"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..>664)
                     /locus_tag="OUB02_003935"
     rRNA            complement(<1..>664)
                     /locus_tag="OUB02_003935"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAKBNN010000415          656 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000415 DAKBNN010000000
VERSION     DAKBNN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.423"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(355..612)
                     /locus_tag="OUB02_003936"
     CDS             complement(355..612)
                     /locus_tag="OUB02_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCT8887612.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
BASE COUNT          211 a          105 c           49 g          291 t
ORIGIN      
        1 caactaatac cttaaaatat ctatttatat tcttaatttt ataaatatat tatgtcatat
       61 taattgctat ttctttattt atatagttta tttgtttatt tttccccttt atacaagcaa
      121 ttgtatcatt ttatctattt tttagccaac aaatattttc gaccattttc tttgtattct
      181 caatatatct ccagtattta acaaattttt ttatagaata tacaatgtat ttaaattaat
      241 aagtagtaaa tttagttaat ttgtctaaaa aaactttata aaacatcttc tttgtcttta
      301 ctcttacgtt taacaaggct attttcatta tgtttaaaca ttattttatt atatttaaac
      361 actattttca ttgagtattc gcttaatatt gcttacacac atattatatc taatagccaa
      421 ttcattgtgg ttgactccat taaattgttt tactatatcc ctatttctag catatctaaa
      481 aagcccctta tacgtaggta tataagtatt tgttcctcca tacaacttgg caacctctat
      541 aaacttatca attccaatta tttctaccat tgcatgaaat tgaactggta tatcctccac
      601 ttttatcttc ataagttaca cctcctttaa gatattttta tagacttaac aaagtt
//
LOCUS       DAKBNN010000416          618 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000416 DAKBNN010000000
VERSION     DAKBNN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.424"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            108..578
                     /locus_tag="OUB02_003937"
     CDS             108..578
                     /locus_tag="OUB02_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HCT8887613.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTVIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          267 a           50 c          116 g          185 t
ORIGIN      
        1 taaaaaagta aaaataaagg attaataaaa ggtactgaag agatagagaa aaaaatgtta
       61 atagaagata ctgaaaaaat agaaataaaa aattaggagg aaaatttatg aaatatcagg
      121 tttcattatt agcagtgaag gatattgaag tatcaaaaaa attttacaaa gaattatttg
      181 agcaagaaat tgaattggac ttaggtaaaa atgtaacatt taaaggagga tttgctatac
      241 aagaagactt cgcatggctt acagggatta atccagatac agttatacaa aaatcaaata
      301 atatggaact ttattttgag gttgatgatt ttgacgaatt tattgaaaaa ctcaattatt
      361 ataaagatgt agagtttgtc cataagccac taaaacatga atggcaacaa agagtagtgc
      421 gtatttatga cccagataag catattattg agataggaga gtctatggaa gttatagcta
      481 gaagatattt atctgaggga tattctgtag aagaaacttc taaaataatt caacatccaa
      541 ttgaatttgt aaagcagtgt gaaaatagta aggaatgatt gtttaaatta taattattta
      601 gtagaaatta attgtggt
//
LOCUS       DAKBNN010000417          610 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000417 DAKBNN010000000
VERSION     DAKBNN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.425"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>610
                     /locus_tag="OUB02_003938"
     rRNA            <1..>610
                     /locus_tag="OUB02_003938"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          127 c          198 g          120 t
ORIGIN      
        1 aggcatctct tgaatatcaa aggtgagcca gtacaggatg gacccgcgtc tgattagcta
       61 gttggtaagg taacggctta ccaaggcgac gatcagtagc cgacctgaga gggtgatcgg
      121 ccacattgga actgagacac ggtccaaact cctacgggag gcagcagtgg ggaatattgc
      181 acaatgggcg aaagcctgat gcagcaacgc cgcgtgagtg atgaaggcct tcgggtcgta
      241 aaactctgtc ctcaaggaag ataatgacgg tacttgagga ggaagccccg gctaactacg
      301 tgccagcagc cgcggtaata cgtagggggc tagcgttatc cggatttact gggcgtaaag
      361 ggtgcgtagg cggtctttca agtcaggagt gaaaggctac ggctcaaccg tagtaagctc
      421 ttgaaactgg gagacttgag tgcaggagag gagagtggaa ttcctagtgt agcggtgaaa
      481 tgcgtagata ttaggaggaa caccagttgc gaaggcggct ctctggactg taactgacgc
      541 tgaggcacga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgctgtaaa
      601 cgatgagtac
//
LOCUS       DAKBNN010000418          561 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000418 DAKBNN010000000
VERSION     DAKBNN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.426"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          208 a           58 c           60 g          235 t
ORIGIN      
        1 atatctcttt atttttataa tcttttatga cttatttata tttttcattt ttataaaaaa
       61 acaataatta agaatataac tatattctac atgtaaaaat ttttttattt aaactcaatt
      121 ttctgataat ttgataattt ttcaaaattt atagttgact tttatttttt actgtattaa
      181 actaattact atacaaaaca gaaaacaaaa ttttcaaaat tattaaatta aaaggagagg
      241 taaatatgaa gactttatta aacaaacaag tacgattttt tagtagccaa tttagttagg
      301 gtttgtacct aagttaagac aaattgtcat aacatagata caagcccctt aggattatga
      361 tttttaagga ttgtatctat gttggctata gtaattgttg ttaaagtttt tattttatca
      421 tcataatata gagccacatg gattatactg tgtggcttat tcgaattttt gccatacagt
      481 tatatatact gtgtggtttt tttatatcaa aatcattttt taaaaccaaa tttaaaaaac
      541 catttaaaat attttcaaag g
//
LOCUS       DAKBNN010000419          549 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000419 DAKBNN010000000
VERSION     DAKBNN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.427"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>549
                     /locus_tag="OUB02_003939"
     rRNA            <1..>549
                     /locus_tag="OUB02_003939"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          155 a          116 c          157 g          121 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagctt
//
LOCUS       DAKBNN010000420          548 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000420 DAKBNN010000000
VERSION     DAKBNN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.428"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            109..>548
                     /locus_tag="OUB02_003940"
     CDS             109..>548
                     /locus_tag="OUB02_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCT8887614.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHT"
BASE COUNT          246 a           52 c          105 g          145 t
ORIGIN      
        1 atccttgggt cgaggatgtt aatgacgaac ttgagagact taaaaaacag gaagatactc
       61 aaaaagagta tgatgattta attcctaata atcaagatgg tgttatagat gaaacataaa
      121 gattattgga gaaagagatt tgaacaatta gaagaagctc aaaataacaa aagtgtaaaa
      181 tattatcttg aattagaaaa gcaatataaa ctagctatga atagtataga aaaagatata
      241 ttagcatggt acaacagatt tgccaaaaat gaaggaatat ctttattaga agctaagaaa
      301 ctactaaata caagagaact agaagagttt aaatggagtg tcgaagaata tattagacat
      361 ggtaaagaaa atgctataaa tcaaaagtgg atgaaagagt tagaaaatgc tagtgcaaga
      421 gttcatataa caagacttga agctttaaag ttacaaatac agcaacaagt agaagtttta
      481 tatggaaatg aacttgatgg tattgataaa ctaatgagag atatttatac aagtggatac
      541 tatcatac
//
LOCUS       DAKBNN010000421          548 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000421 DAKBNN010000000
VERSION     DAKBNN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.429"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          202 a           51 c           58 g          237 t
ORIGIN      
        1 taaaatttaa tttatcatat attatataga atataagtat tacacatatt atagccataa
       61 taagtattat ttgtattgca tgtttgctaa taagctgatt attagacaca atagactcaa
      121 agttatttat ggagttgttt ttatagagtt tgcataaaga atagactggt gttgtgtatg
      181 taatttgtaa aattatacaa ataaataaaa aaaacatcaa ttttttttgt aaattcctca
      241 taaaaatttc ctcccattaa aacacctcta ttttattagc aaataagtac atctcaaaag
      301 tttccatttc ttgtaaaatg gatagtttat atctacttta attttgtaaa attttctgct
      361 actataatta tagaatgtag catattcatt gtcttaacta ttgatttata tatttcatat
      421 aggtataata gaaaataaat tggattattt tggcatataa ttatttatta aaaggtgatt
      481 atttttgttt aaattgtctg aaaaatttta tttttatatt ataattttga tattttcttt
      541 tcaaaact
//
LOCUS       DAKBNN010000422          545 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000422 DAKBNN010000000
VERSION     DAKBNN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.430"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(128..>545)
                     /locus_tag="OUB02_003941"
     CDS             complement(128..>545)
                     /locus_tag="OUB02_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HCT8887615.1"
                     /translation="NKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFLEMIDNENS
                     YIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPNITKYKENL
                     INRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT          189 a           59 c           49 g          248 t
ORIGIN      
        1 gttaatatat tttacttata taaatttttc ataattaatt tatacatata aattagcacc
       61 attgttgttt tattatctta gttataaata attgtaacta gataatattc aatttctata
      121 ttaatattta actaatatta gataatgtat cgtttatatc cttttctact aatggtttca
      181 tattattatt ataaatactt atatctgcat tacttaattc catttttatg cgattaataa
      241 gattttcttt atactttgta atattaggta tggatttaat aaacattctt gatacagaag
      301 gttttttatc tacgatatga cttagtatac tatctatatt tatttcaatt ttattatcaa
      361 tatcccactg agcatttgca gagattaata acaatcccct tgttcgaata tatgagtttt
      421 cgttatcaat catttctagg aatgagtcaa aaaaacaata tgtaatattt gattttaaac
      481 tttcattttc taagaataaa aaagttttat atgcttcatt atgatttttt gaatataatt
      541 tatta
//
LOCUS       DAKBNN010000423          529 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000423 DAKBNN010000000
VERSION     DAKBNN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.431"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          179 a           61 c           37 g          252 t
ORIGIN      
        1 tataaatata ggtactgaac ctatctttat ttcatttaaa aaaattttta taagtattac
       61 tataatttat caatcttttt ttgtctacat ttttacgatt atttacgatt tatttttcac
      121 atatgtatat ttaatcttgc caactaaata tacatttttt ataagcattt actctttata
      181 taataaaact taagttgttc atgtagtctt cttctggttt aattattaaa atttcaaatt
      241 atattattat ttctacacat tattacatcc tttagtacaa ttataacata attttacaat
      301 ttaatgatat ctattcaatg tcttttttta ctctacaaca tattttattt tagagtttaa
      361 tactatatat attttttata tacagccttg tcatttttta gattgtcaag agttattgaa
      421 gtattttatg tagatttaga ttttatttcc taatttctca tttaacaaga attgcttatg
      481 ataaaaaata atatcatctc aataagattc ttgtaaatat atctatatt
//
LOCUS       DAKBNN010000424          528 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000424 DAKBNN010000000
VERSION     DAKBNN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.432"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..267)
                     /locus_tag="OUB02_003942"
     CDS             complement(<1..267)
                     /locus_tag="OUB02_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCT8887616.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTE"
BASE COUNT          201 a           69 c           49 g          209 t
ORIGIN      
        1 ctcagttgat tgattataat ttaaagttgt atccaatggc atatcatagc atttatactt
       61 taatgtaatg ctatctgcat ctggtttttc tttaaactta acactgactt ttgaggtact
      121 acctaaaacg atactttttt tctctaagtt tatatttaaa tcagcgttat tttccatagc
      181 attagattca gtagcaaatg ccattgtagg tgtgacgatt gccattgaaa gaattcctgt
      241 tgttaataat tttgtatttc ttctcatatt aatctcctta cttaacataa atttctacct
      301 tgttttttaa tcttttatct tttgaaaata tatcaaagtg tttttaccca aaattataaa
      361 taagtacata aaaaataaac ttctaaaaaa attataaaaa caaaaaataa aaaaattaaa
      421 tatattgaat atatttaatt atcttcacta tatcatttat tgccaaaaaa atctataaaa
      481 gacaaataaa aacatataat aattgtaggt ttattaaatt taatacaa
//
LOCUS       DAKBNN010000425          522 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000425 DAKBNN010000000
VERSION     DAKBNN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.433"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>522
                     /locus_tag="OUB02_003943"
     CDS             <1..>522
                     /locus_tag="OUB02_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCT8887617.1"
                     /translation="NNYHKLLVDQKVGYSVGNPIVFNADDDNLTKLLNDLLGEEFDDT
                     ITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIPIWDSKRQRELVAFIRFYYIE
                     DIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMTDIQEGHFRINNKEQGWG
                     KVPFIPFKNNEKCV"
BASE COUNT          210 a           53 c           99 g          160 t
ORIGIN      
        1 aacaactacc ataagctttt agttgaccaa aaggtaggct atagtgtcgg aaatccaatc
       61 gtatttaatg cagatgatga taatctcact aagcttttaa atgacttact aggagaagag
      121 tttgacgata caataacaga actatatctc aatgctagta ataaaggggt tgaatggtta
      181 catccatata ttaatagaaa aggtgagttt aaatatgtaa taattccagc tgaagaagca
      241 attcctattt gggatagtaa aagacagagg gaattagttg catttattag gttttattat
      301 attgaagata tagatggaaa taaaataaaa agagttgagt actacacaga aaatgatgta
      361 acttatttta ttgaaagagg taatagtttt attcaagaat ttttatatga tgaatacgga
      421 aaaatgactg atatacaaga aggtcatttt agaataaata acaaagaaca gggatggggt
      481 aaagttccat ttataccttt taaaaataat gaaaagtgtg tc
//
LOCUS       DAKBNN010000426          511 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000426 DAKBNN010000000
VERSION     DAKBNN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.434"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..>511)
                     /locus_tag="OUB02_003944"
     CDS             complement(<1..>511)
                     /locus_tag="OUB02_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCT8887618.1"
                     /translation="IKEIETLYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVN
                     FLKARKYADLLKDMQVSSLCNIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQK
                     YRASNNITKILIKTRNYPVAINNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKS
                     NEKSFEQSIC"
BASE COUNT          171 a           73 c           40 g          227 t
ORIGIN      
        1 agcatattga ttgttcaaaa gatttttcat tactttttgc ataatacatg gctaaactta
       61 taaataaatc aaacttctct acttctttta agctatgatt tttaattttt tctagatata
      121 cttcagattt tgctaaatta tttattgcga caggataatt tctagtcttt attaatattt
      181 tagttatgtt gtttgatgct ctatattttt gaattatatt tacatttcta tactttttat
      241 tgcttattac ttcattgaaa tattccatgg aacattcata attttcaaac gaacattcac
      301 actcacctat ctttatatta catagtgaag atacttgcat atcctttagt aaatctgcat
      361 attttcttgc ttttaaaaaa tttaccttag caattagagc atcatccaaa aacaaaaaat
      421 atattttacc aataataaaa aaatatattt ttttacaatt aaaatcgtta atttctttta
      481 tctttatata caacgtttct atctctttaa t
//
LOCUS       DAKBNN010000427          479 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000427 DAKBNN010000000
VERSION     DAKBNN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.435"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(36..242)
                     /locus_tag="OUB02_003945"
     CDS             complement(36..242)
                     /locus_tag="OUB02_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887619.1"
                     /translation="MFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSTLDVAVLIGFNS
                     QNYYSIIFKKFNNFTPIEYKNIYN"
BASE COUNT          177 a           47 c           45 g          210 t
ORIGIN      
        1 tgtaaaaaaa taacatcttt ttagaatgat attttttagt tataaatatt tttatattca
       61 attggtgtaa agttattaaa ctttttaaaa attatactat agtaattttg actattgaat
      121 ccaattagta cagcaacgtc taaagtagac aagtcttttt ttaatatata ctttttgctt
      181 ttttcaactc taactttact taaaaaatta gtaaaagtat atccactttt tttcttaaac
      241 aaagaaaaaa acaactttta ttaagttgta attcattaca tatattactt actgttatat
      301 cttctgagta atttttgtga atataatcta tagttttctt taagttttct ttacataaaa
      361 ctaagagttg gtctatgttt cattttatca aggcatattt attaaaaaat caatctctat
      421 ttgttaaaat aatttatgaa tgatgttaga aactgtaata tattggatat aatataatt
//
LOCUS       DAKBNN010000428          462 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000428 DAKBNN010000000
VERSION     DAKBNN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.436"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            189..422
                     /locus_tag="OUB02_003946"
     CDS             189..422
                     /locus_tag="OUB02_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCT8887620.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT          179 a           51 c           46 g          186 t
ORIGIN      
        1 aaactttcga aaattctgaa ttttatatag cattttttat tatctagtgt tataattaag
       61 atataaataa tatattattc taattttcat atctatttgc ttatttttta acttagtttc
      121 taatagaagt aatcatattt atctttttaa aagtttcaac tttaataaaa aataaaggag
      181 ttgattttat ggctactact acaaaaaacc gtttttacga ttatgttcaa gaaaacttaa
      241 ataaatatgt tgaagtacat tcttacttta gcagttgtcc tctatatggt gaaattatag
      301 aagcagatag cttatcaatt acactttgct caagatatat agatgaatct aatccaactt
      361 taaatgaaac ttacacatta tatttaccat taagttcaat aatatctatt agaacactat
      421 aaaaagttaa ttaaatatgg gttatttata tataaaatgt aa
//
LOCUS       DAKBNN010000429          446 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000429 DAKBNN010000000
VERSION     DAKBNN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.437"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(138..338)
                     /locus_tag="OUB02_003947"
     CDS             complement(138..338)
                     /locus_tag="OUB02_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCT8887621.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT          170 a           70 c           37 g          169 t
ORIGIN      
        1 ttttaaaaaa gttagtttaa gtttgacgct tttctaaagt aacttcttat ttttgtaatt
       61 aaaaataaat ataataaaca acaaaaaaga gccctcgata gctccttttc taaaatatat
      121 atatttaaat taaataatta tattctattt acgttttcag cttgaggtcc tctattacct
      181 tgagttatat caaagctaac cttttcgcct tcttctaaag ttttgtatcc atcattttgt
      241 atagctgaga aatgtacgaa aacatcatct tctccctcta cagatatgaa cccaaatcct
      301 ttttcattgt taaaccattt tactattccg tttttcatta taagaaaacc tccgcattat
      361 taaaaaatac aaactaaaaa aataagtctg ttctattaat ataacataaa ttttaaataa
      421 aagcaaataa aatttttaaa aaaatt
//
LOCUS       DAKBNN010000430          444 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000430 DAKBNN010000000
VERSION     DAKBNN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.438"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            124..288
                     /locus_tag="OUB02_003948"
     CDS             124..288
                     /locus_tag="OUB02_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887622.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          182 a           26 c           75 g          161 t
ORIGIN      
        1 taagaatttt gaaggtaaag ctagtttaaa aaaataaagg gactaatttg tctaacgttt
       61 attggtgaaa aataagtcta tagaagttat aataaatata taaaaatgta tgagaggtgt
      121 tatatgtttg gtaaaccaaa agaaagtgtt agatttttag actttaatga tgtagcagaa
      181 aagtttggag agaaaaaggc tgttgcaatt tttgatgcca tagctggaaa aaaatctaaa
      241 gaacatatta tcaaagatat agaaaaatta gtaaaaaatg aagtttagat atttataagg
      301 tatattgagc ttgagggtag ctctttatac cttctttttt atgattttta gataatattt
      361 taaaatttaa ataagaaact tattgttata gagtaatatt tatatagaaa taagtcttta
      421 taatttaaac ttgtaattag ttta
//
LOCUS       DAKBNN010000431          430 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000431 DAKBNN010000000
VERSION     DAKBNN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.439"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            36..>430
                     /locus_tag="OUB02_003949"
     CDS             36..>430
                     /locus_tag="OUB02_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCT8887623.1"
                     /translation="MNITKQKSQKTFDKQAADYDTNIQGEHARKLYKPIIENLKNKNI
                     HSILDLGCGTGALLKEIKELNIAEQLFGIDISPNMLEIAKNKLGNDATLILGDSERLP
                     FEDSSFDAIVCNDSFHHYPQPDIVEKEVS"
BASE COUNT          171 a           59 c           75 g          125 t
ORIGIN      
        1 cacgagatat aattttagtg aaaggagttg attacatgaa tattacgaaa caaaaatcac
       61 aaaagacttt tgataagcaa gctgccgatt atgatacaaa tattcagggg gaacatgctc
      121 gaaaactcta taagccgatt atagaaaatt taaaaaataa gaatatacat tctatattag
      181 atttaggttg tggaacgggt gcgttactta aagaaataaa agaattaaat atagcagaac
      241 aactatttgg aatagatata tctcccaata tgcttgaaat tgcaaaaaat aaactaggaa
      301 atgatgcaac acttatctta ggcgactctg agagattacc ttttgaagat agttcttttg
      361 acgccattgt atgtaatgac tcatttcatc attatccaca accagatatt gtagagaaag
      421 aagtgtctag
//
LOCUS       DAKBNN010000432          428 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000432 DAKBNN010000000
VERSION     DAKBNN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.440"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          174 a           33 c           40 g          181 t
ORIGIN      
        1 tgtagtataa ataatgatta gaaacgtttc gagttaatta ctatttattt taaactatgt
       61 cttaaataaa atatatattt attgttatat aataagcaat aaattgcttg ataatatatt
      121 tttatgtgaa aaaaacatca aaaaaatact tttaagaatt taatttcagg tgaaataatg
      181 gagaaatcat caattaaaat attaattggt ttatttatta taggagtaat tattatttta
      241 atcttcctta atagaactaa aactaaaaat tatctttatc aaatttttaa tgttataata
      301 aacagtacta aattaatctt taattatagc tggatatata tttttttatg cattattttt
      361 tcagtatttc aaggtttttt tccaataata tctttgttaa taatgcaaaa aatgataaat
      421 gcaattca
//
LOCUS       DAKBNN010000433          419 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000433 DAKBNN010000000
VERSION     DAKBNN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.441"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            68..>419
                     /locus_tag="OUB02_003950"
     CDS             68..>419
                     /locus_tag="OUB02_003950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887624.1"
                     /translation="MRVTIEKINPIAIKKLINMLYLTIKLDIFKLSNPNKYVIELITK
                     ITITIKLRPFKLFFKSALLSDICVDIEYIKNINIKYFNEYTLGVNNIILIKLEISPKI
                     MCIKPFIFKLLIILT"
BASE COUNT          185 a           49 c           41 g          144 t
ORIGIN      
        1 agtaaaatat gtatttattt aactaaagtt tttaaatggg ctgactaagc ttaaaatata
       61 ggatattatg agagttacta ttgaaaaaat aaaccctatt gccataaaga aacttataaa
      121 tatgttatac ttaactatta aactggatat attcaagtta tcaaatccaa ataagtatgt
      181 aattgaactt attacaaaaa taactataac tataaagctc agaccattta agctattttt
      241 taaatcagca ctactaagtg acatatgtgt agatatagaa tacattaaaa acataaatat
      301 aaaatacttc aatgaatata cactaggtgt taacaacatt attcttataa aattggaaat
      361 atcacctaaa ataatgtgta taaaaccatt tatatttaaa ttattaatca tacttacat
//
LOCUS       DAKBNN010000434          408 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000434 DAKBNN010000000
VERSION     DAKBNN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.442"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>408
                     /locus_tag="OUB02_003951"
     CDS             <1..>408
                     /locus_tag="OUB02_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCT8887625.1"
                     /translation="SATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQMRKE
                     QDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVLRIG
                     FKDGELYICNEIYVHEMDTSEIIKIANSKGLEKN"
BASE COUNT          165 a           47 c           79 g          117 t
ORIGIN      
        1 tcagctactc attggataaa aagaaagtac tttgactata aaaatgatga tatatttact
       61 catcatagta cttatctaca aaatagattc atagatgagg cttactacag aagaatgcaa
      121 atgagaaaag agcaagaccc agaagggtac aaagtctatg gtcttggaga atggggagaa
      181 actggtggag caatacttaa aaactatgtt atacatgaat ttcctacaga atttgaatac
      241 tttgataata tgagactgtc acaagacttt ggctttaatc atgcaaatgt agtactcaga
      301 attggtttta aggatggaga gttatatata tgtaatgaaa tatatgtaca tgaaatggat
      361 acatctgaaa taataaagat tgcaaacagt aaaggtttag aaaagaat
//
LOCUS       DAKBNN010000435          408 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000435 DAKBNN010000000
VERSION     DAKBNN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.443"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>408
                     /locus_tag="OUB02_003952"
     CDS             <1..>408
                     /locus_tag="OUB02_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCT8887626.1"
                     /translation="SATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQMRKE
                     QDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVLRIG
                     FKDGELYICNEIYVHEMDTSEIIKIANSKGLEKN"
BASE COUNT          163 a           46 c           80 g          119 t
ORIGIN      
        1 tcagctactc attggataaa aagaaagtat tttgactata aaaatgatga tatatttact
       61 catcatagta cttatctaca aaatagattc atagatgagg cttactacag aagaatgcaa
      121 atgagaaaag agcaagaccc agaagggtac aaagtctatg gtcttggaga atggggagaa
      181 actggtggag caatacttaa aaattatgtt atacatgaat ttcctacaga atttgaatac
      241 tttgataata tgaggttatc acaagacttt ggatttaacc atgcaaatgt agtacttaga
      301 attggcttta aggatggaga gttatatata tgtaatgaaa tatatgtaca tgagatggat
      361 acctctgaaa tcataaagat tgcaaacagt aaaggtttag aaaagaat
//
LOCUS       DAKBNN010000436          393 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000436 DAKBNN010000000
VERSION     DAKBNN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.444"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          177 a           34 c           50 g          132 t
ORIGIN      
        1 ctattcaatt atttaacctc ttaaattata tttagatata ggagtcttat gataaaagta
       61 agcttatgtg ttatttgtag taaatataaa gtgcatatag tgataaaaca aaagtttcat
      121 ataaatgaag aaaaaaatat aaaataattt atttaactac ttaaaaataa actaaaaggg
      181 ataataaaat agcttttagg ttatggtaat aaaggtattt aaaaagagtg cataagcact
      241 ccaattgtga gaaatacata cacaaatcat tattacaaca atgaaaaatg tacatataag
      301 agacgttttg tatttgtttt aagtgataat attttaatac taactaaata attatcacaa
      361 tagtaaaaaa gaaaaaggag tattttgtac tcc
//
LOCUS       DAKBNN010000437          391 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000437 DAKBNN010000000
VERSION     DAKBNN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.445"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            154..270
                     /gene="rrf"
                     /locus_tag="OUB02_003953"
     rRNA            154..270
                     /gene="rrf"
                     /locus_tag="OUB02_003953"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            276..364
                     /locus_tag="OUB02_003954"
     tRNA            276..364
                     /locus_tag="OUB02_003954"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
BASE COUNT          131 a           69 c           83 g          108 t
ORIGIN      
        1 taactctata tttttaaact tgtcaaaact tatggatttt taatagtttt ataaggcaaa
       61 aaatcgaata aaaaaagtat tgacacaata agacaaagat gttattataa tacttgtctt
      121 agaaaaaaag ttaaatgaaa atttagacaa taatctggtt ataatagcag agaggataca
      181 cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac ttggtgggaa
      241 actgcctggg agagtaggac atagccaggt gatgtgccga agtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc
      361 accacaaatt attgtgggcc attagctcag t
//
LOCUS       DAKBNN010000438          375 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000438 DAKBNN010000000
VERSION     DAKBNN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.446"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          123 a           48 c           26 g          178 t
ORIGIN      
        1 ctttctttta attctttagg atggatttaa attttctata aaaatcatac tattttagct
       61 gtttacttgc ttatttaata gttttatttc tttgattaaa tatctttcag aatctaagcc
      121 agatgtctca aaaaaattga tttataatat aatcattttt tctgctattc ttatttttcc
      181 agttttagtt tcatattaaa atatactttt catagacact gcattctttc atactcattt
      241 tcacatttaa ctatacaagt agtcttactc atttatgtac tatactttat taatatttat
      301 taagaaaaac tcaaagattt aaaaatatat attttctata aagtatattt atgaattatt
      361 tcatatcttc tttta
//
LOCUS       DAKBNN010000439          364 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000439 DAKBNN010000000
VERSION     DAKBNN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.447"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="OUB02_003955"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="OUB02_003955"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="OUB02_003956"
     tRNA            291..362
                     /locus_tag="OUB02_003956"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAKBNN010000440          333 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000440 DAKBNN010000000
VERSION     DAKBNN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.448"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            62..>333
                     /locus_tag="OUB02_003957"
     CDS             62..>333
                     /locus_tag="OUB02_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCT8887627.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADK"
BASE COUNT          137 a           33 c           74 g           89 t
ORIGIN      
        1 gaagttgata acctaagtaa agatgatatt ggagatgatg agttgaaaat aagtgtagat
       61 tatggtgata gaaatgatag ttagagtaaa ttttaatcca gatttcaagg aagccaattt
      121 tactaaaaaa agatacagag caatgaaagg ttcagcaggg agtggaaaat ctgttaatgt
      181 agcacaagac tatatactaa agttaggaga taagaagtat caaggagcta atctattagt
      241 agttagaaag tcagaagcta cacataagta ttcaacgtat gcagagctta caggagctat
      301 aaatcgtatt tatggtaaac aagctgataa gta
//
LOCUS       DAKBNN010000441          331 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000441 DAKBNN010000000
VERSION     DAKBNN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.449"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          117 a           34 c           34 g          146 t
ORIGIN      
        1 tattcttttc agttaataaa ataggtacat ttttagtaca tgaaaataga gttgcttaca
       61 gggtatatag gattgatgta ctatttacta atattacatt ttcaactgat tcaacccctc
      121 ttttacttac ataatttaag tatatttttc tggattttta aatttatgta ctttaaaatg
      181 gatatactta ttttcattga ttttgtaaga attaataaaa aatacgatta cactacaaaa
      241 ttagaatgtc aatgtttata aataaaaata atatttttta ttttaattat tacatccata
      301 gtttccattt gttataaaag ggttcttttt t
//
LOCUS       DAKBNN010000442          315 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000442 DAKBNN010000000
VERSION     DAKBNN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.450"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..268)
                     /locus_tag="OUB02_003958"
     rRNA            complement(<1..268)
                     /locus_tag="OUB02_003958"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a           82 c           53 g          103 t
ORIGIN      
        1 ggctaatccg cgttcgctcg ccgctactta cggaatcgaa tttctttctc ttccttcagg
       61 tacttagatg tttcagttcc ctgagttccc ctcattaagc tatgtattca cttaatgata
      121 cttagacatt actctaagtg agtttcctca ttcggaaatc ttcggatcaa agtttacgtg
      181 caactccccg aagcttatcg cagcttatca cgtccttcat cggctcctag tgccaaggca
      241 tccgccctgc acccttaata acttgaccag ttaaaaaggt ttgatagatt agaagctatc
      301 aacttcgata atttt
//
LOCUS       DAKBNN010000443          298 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000443 DAKBNN010000000
VERSION     DAKBNN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.451"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          143 a           18 c           32 g          105 t
ORIGIN      
        1 tctatattaa ttgtaaatcc tgatactaga aaaagtataa ttaaattcat aaaattaaaa
       61 aaataatttt tagaaaggta ttatttagaa aatcatgaga ttgctaagta atacctttca
      121 ttattatgaa aactgtattt gttgttgaga aaacatatat aatatatgaa ttttctaaat
      181 agtataaaaa taatagaata ttgaaaaaaa attgacaaat gaatgtatag gtattaaaat
      241 atattaaata tatacataaa aagtggttta ctaacaaata aatgaaatat atgttaac
//
LOCUS       DAKBNN010000444          296 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000444 DAKBNN010000000
VERSION     DAKBNN010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.452"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          135 a           30 c           32 g           99 t
ORIGIN      
        1 atatataaaa ttcagaaaaa aattagttta cataataaaa ataaagtcaa ctaaaattat
       61 tgattttaga ttaaagtacc agattaagcg tttaagtaga ttttaagctt gatttgatta
      121 gaaaggtaca aactatcgcc gaaaaaaatt aaacccattt aaaaagcaat ataaaagctt
      181 gttatttttg agaaaaatag accctctaaa ctcaattaga taaaaataaa atacagaaaa
      241 ttatatctct gaatatctca agtaattaat tttaaatttt acgatatttt gtttga
//
LOCUS       DAKBNN010000445          279 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000445 DAKBNN010000000
VERSION     DAKBNN010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.454"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..>279)
                     /locus_tag="OUB02_003959"
     rRNA            complement(<1..>279)
                     /locus_tag="OUB02_003959"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           57 a           77 c           57 g           88 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctg
//
LOCUS       DAKBNN010000446          267 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000446 DAKBNN010000000
VERSION     DAKBNN010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.455"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>267
                     /locus_tag="OUB02_003960"
     CDS             <1..>267
                     /locus_tag="OUB02_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCT8887628.1"
                     /translation="EPDRIKMWKSAGYKAKGVKKGPGSVKAQIDYLKQLRIHVHPSCT
                     NTIKEIQQWKWKQDERTGLYLDEPVEFMDDAMAALRYSIDNKLK"
BASE COUNT          114 a           31 c           54 g           68 t
ORIGIN      
        1 ctgaaccaga tagaattaag atgtggaaga gtgcaggata taaagctaaa ggagttaaaa
       61 aaggaccagg aagtgttaaa gctcaaatag attatttgaa acaattaaga atacatgtac
      121 atcctagttg cactaatacc ataaaagaaa tacaacaatg gaaatggaaa caagatgaaa
      181 gaactggatt atatcttgat gaaccagttg agtttatgga tgatgcaatg gctgctctta
      241 gatattctat agataataag cttaaaa
//
LOCUS       DAKBNN010000447          257 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000447 DAKBNN010000000
VERSION     DAKBNN010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.456"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..>257
                     /gene="tuf"
                     /locus_tag="OUB02_003961"
     CDS             <1..>257
                     /gene="tuf"
                     /locus_tag="OUB02_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCT8887629.1"
                     /translation="MITGAAQMDGAILVCSATDGPMPQTREHILLSRQVGVPYIVVFL
                     NKCDMVDDEELLELVEMEVRDLLTEYDFPGDDTPIVRGSAL"
BASE COUNT          101 a           34 c           57 g           65 t
ORIGIN      
        1 atgataacag gagcagcaca aatggacgga gcaatattag tttgttcagc aacagatgga
       61 ccaatgccac aaacaagaga gcatatacta ttatcaagac aagttggagt accatatata
      121 gtagtattct taaacaaatg tgacatggta gatgatgaag agttattaga gttagtagag
      181 atggaagtaa gagatttatt aacagaatat gatttcccag gagatgacac tccaatagta
      241 agaggttcag cattaat
//
LOCUS       DAKBNN010000448          250 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000448 DAKBNN010000000
VERSION     DAKBNN010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.457"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          102 a           30 c           16 g          102 t
ORIGIN      
        1 acattatcat ctctctgtta tatatttatt atatagccgt atatcgataa agtaaataaa
       61 tcccatattt aaaataatat gtttgacaat aaattattat aatgtatatt taccataata
      121 atttgtcaac cttgaaagtt acttctataa ttattgttaa ataattcttc taaaaaaaat
      181 acctataaat tttattttct gaaatacata gttataacta tttttatcac gtataagaat
      241 aaaaattcca
//
LOCUS       DAKBNN010000449          239 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000449 DAKBNN010000000
VERSION     DAKBNN010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.denovo.459"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
BASE COUNT          113 a           15 c           18 g           93 t
ORIGIN      
        1 catctctatg cttaaaaaag aaaaataaat ctattaaaat aacataataa aaaaaatatt
       61 aagaataaaa atataattat aagtatttat acaaatctag ttgattttta atataaatgt
      121 aaatacaatt atagctaaat gaataattgt attaatattt atttgtaata tgcttaatat
      181 aatttgcaat tttacataag tttgttaagt acataactaa tttaagaata tgtatttat
//
LOCUS       DAKBNN010000450        13219 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.106, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000450 DAKBNN010000000
VERSION     DAKBNN010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.106"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..1393
                     /locus_tag="OUB02_003962"
     CDS             <1..1393
                     /locus_tag="OUB02_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8887630.1"
                     /translation="PIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFL
                     GLAVTKATYTSSVIPIIIAIWALSYVEKVLEKYIPEIIKIIMVPTLSLVVMLPATLFL
                     FGPIGIYIGNVINFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQT
                     GRQNLLAFAGAANFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLR
                     LKKPMVCAVICGAFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIA
                     FFGSAISTYLVGFEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFA
                     SKAMGEGIAVLPTKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNG
                     KYFTKHVNQGDLVKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVS
                     TNDNIISLVLAGN"
     gene            1703..3109
                     /locus_tag="OUB02_003963"
     CDS             1703..3109
                     /locus_tag="OUB02_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCT8887631.1"
                     /translation="MNTGFPKDFLLGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHSFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3137..3985
                     /locus_tag="OUB02_003964"
     CDS             3137..3985
                     /locus_tag="OUB02_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCT8887632.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            4646..5338
                     /locus_tag="OUB02_003965"
     CDS             4646..5338
                     /locus_tag="OUB02_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887633.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            5335..6270
                     /locus_tag="OUB02_003966"
     CDS             5335..6270
                     /locus_tag="OUB02_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8887634.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            6418..7341
                     /locus_tag="OUB02_003967"
     CDS             6418..7341
                     /locus_tag="OUB02_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887635.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            7325..8095
                     /locus_tag="OUB02_003968"
     CDS             7325..8095
                     /locus_tag="OUB02_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887636.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8105..8887
                     /locus_tag="OUB02_003969"
     CDS             8105..8887
                     /locus_tag="OUB02_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887637.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            9264..9800
                     /locus_tag="OUB02_003970"
     CDS             9264..9800
                     /locus_tag="OUB02_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HCT8887638.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            9832..10221
                     /locus_tag="OUB02_003971"
     CDS             9832..10221
                     /locus_tag="OUB02_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCT8887639.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            10258..10644
                     /locus_tag="OUB02_003972"
     CDS             10258..10644
                     /locus_tag="OUB02_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HCT8887640.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            10771..12213
                     /locus_tag="OUB02_003973"
     CDS             10771..12213
                     /locus_tag="OUB02_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCT8887641.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            12510..>13219
                     /locus_tag="OUB02_003974"
     CDS             12510..>13219
                     /locus_tag="OUB02_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCT8887642.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEE"
BASE COUNT         4737 a         1652 c         2519 g         4311 t
ORIGIN      
        1 gcctattata ctaggatata ctgcggctaa agtatttaat gcaaataaaa taatatctat
       61 ggtattagga gctacacttt gttatccaac ttttactgct ttgatggcag gtgaagaatc
      121 tgttagattt ttaggattgg cagtaacaaa agctacgtat acatcatctg ttattccaat
      181 aattattgct atatgggctc tttcatacgt tgaaaaagta ttagaaaagt atatacctga
      241 gataattaaa ataataatgg ttccaacttt atcattggtt gtaatgttac ctgcaacatt
      301 atttttattt ggaccaatcg gaatttatat aggaaatgtc ataaactttt cttataagta
      361 tatttatgaa ttaagcccag cattgtgtgg agcatttgta ggtgggttat ggtgtgtgtt
      421 ggttatattt ggtgctcata gagcattatt accaataggt ataagtgatg ttgctcaaac
      481 aggaagacag aatctattgg cgtttgcagg agctgcaaac ttttcacaag caggggctgc
      541 tctaggagta ttctttaaaa ctaaaaatca aggactaaaa acaatttcta tgtctgcaac
      601 tataacagct ttatttggta taactgaacc tgcaatatac ggtgccaatc ttagattaaa
      661 aaaacctatg gtatgtgccg taatatgtgg tgcatttgga ggtgcgataa tgggaatggg
      721 tggagcttat ggtaatgcat ttgcaaacca aggtgtttta actatacctg tatatgctga
      781 agcaggtgca ttaggtttct taagttatct aggtggttgt gctatagcat tttttggttc
      841 agctatttca acttatctag ttggatttga agatttggaa gagtcttcta ataaggaaaa
      901 ttcatctata aaagtagaaa cgaaggatgg tgttattgat ataacttcac ctgtagaggg
      961 cgaatgtata gaattgtcag aagtaaagga tgatgtattt gcttcaaaag ctatgggaga
     1021 aggtatagct gtgttaccta caaaaggagt aataacagcc cctactgatt gtgaagtagc
     1081 ttctttattt ccaacattac atgcaatagg attaaaatta gataatggag cagaaatgtt
     1141 aatacatgtt ggaatcaata cagtagagtt aaatggtaaa tactttacaa aacatgttaa
     1201 tcaaggagat ttagtgaaaa aaggtgataa acttatatcc tttgatatag ataaaataaa
     1261 aaaggctggt tatgatgtaa ctacgccagt tatagtaaac aatacatttg attttgggca
     1321 agtagtttct tgtaaatcta gttatgtttc aaccaatgat aatataattt cattagtatt
     1381 agctggtaat taagttaatt tgactagaga taagtaaatt gatttaaaag gaaataagta
     1441 aatcaacaat taaatacatc taaaagtaag tttattgcaa tgaattgtta aagaaaataa
     1501 ttaatatgga gtaaatctat tgcaatgaag taacaaagaa aataattaat atagagtaag
     1561 tttattgtaa tgaattgtta aagaaaataa ttaatatagg gtaaatctat ctcaatgaaa
     1621 taacaaagaa aagagttaat atagagtgaa cttattgcaa tgaatcaaca aagaaaataa
     1681 ttaataaaaa ggagaaatat ttatgaatac aggatttcca aaagactttt tattgggagc
     1741 atcatcttct gcatttcaag tggaaggagc ttgggataaa gacaataaag gaaaaaccgt
     1801 agccgactat aattctttta aaaagtctca tttacaggca gatactaaag tcgcatcaga
     1861 cttttaccat aattatgaag aagatattga attaatgaaa gaattaggga tgaaaacata
     1921 tcgtttttca atttcatggg caagaataat accagatggt gaaggagaga taaaccagaa
     1981 aggtttagaa ttttataata agataataga caaactaata gagtgtgata ttgagccatt
     2041 cgtaacttta taccattttg accttccatt taaattagta gagaaatata atggttggga
     2101 aagtagagaa actgtctatg cttttgaaag atttgctaaa atatgcttta agcattttgg
     2161 agatagggta aagtattggc aacctcataa tgaacagaat ttaattgtta gagtggagga
     2221 gagaattaat atatatgatg aaacagattc atggaagata gataaaatgc gtgctcagat
     2281 ggattataat ctttgcttag cacatgcatt agcagtcaat gcatgtcatg agatgataaa
     2341 agaaagtaaa attggagcag cagtatcatc atctgtaaca tatcctctta cttctaagcc
     2401 agaagatgta tatgcagcta gaatgaatga taattttaaa gtgtactata tgcttgatat
     2461 gcatcattat ggagaatatc cagggtatta tatgaagtat cttgaaaaaa gaaatatagt
     2521 tcctcacatg gaagatgggg ataaagagat acttaaaaaa gctaaaatgg attttatagc
     2581 tgtaaattat tataggacaa attgtgcaga agctttacct gaagattcac aacattcatt
     2641 tggattaaga gaaggaactg ttgattttag tatgtatgga ctatttaaaa tgtcaatgaa
     2701 tcctaactta gaggcctctg agtatggagc agcaatagac ccatcaggtc ttagagttgc
     2761 cttaaatgaa tattggcaaa gatatcactt acctgttata ataacagaaa atggtctagg
     2821 agctaaagat atattagaag atggaaaaat tcatgatgac tatcgtatag actatttaag
     2881 aagtcatata aatgcatgta aactagctat tgaagatggt gtagagatga ttggatattg
     2941 tccatggtca tttacagacc ttttaagttc ttctcaaggt tttaataagc gttatggact
     3001 tgtttatata aatagaactg accatgaaat tttagattta aaaagaatca agaaagatag
     3061 tttttactgg tataaagaag ttatagagaa caatggaata gtaaaataaa cataaatata
     3121 tattgagggt tttagtatgt acattacaaa ggtattaaat aattcgttgc tacttgcaaa
     3181 ggatgataat gatgaagaaa taatattgat ggggaaaggc attggccata attataaggc
     3241 cgggtctgag ttaaataaaa aagatataga aaagattttt gttcttcatg atgaaaatat
     3301 aaaaaagagt attatacaat tggcaacaga aatagatgca gaatattttg gtatagctca
     3361 aatgataatt gcatatggaa tagaaaaata taacttaaaa cttatgaacc atatatattt
     3421 ggccttaact gaccacattg cctttgcagt gagacgtttt aaagaaggta taatgattga
     3481 aaatcactat ttgttcgaaa tcaaggaatt taatccaaaa gagtatgata taggaaaata
     3541 tgctattact gtttttaagg aggttcttgg tttagaccta ccagaagaag agattggtaa
     3601 tatagcaggc cattttataa atgcacaaca agataatcct tatagtgata gaaataagag
     3661 aagtgctaaa attgtaaatg ctattttgca aatagttcat tatcactttt ctattgtata
     3721 taataaagag tcattttatt ataggagatt tgtaatgcat ctaaaggcct tttcacaaag
     3781 atttctggct aatgaaccat caagagaaaa aatagatttt atatatgagc aagttcaaaa
     3841 aaattgtaag gaagagtatg aatgtgtgaa aaaggtagga gcttatatgt ataaggaata
     3901 ctccagagaa ttaccaagac aagaggagtt atatttgatg attcatattc ataagatttt
     3961 aggagaatta tgcgaaaata gttagaatat cttaatatgc tagtaatatt ataaaaaatt
     4021 aaaaattaac tgcactttaa atatgaataa ttgcattact taagggggtt gtcttaaaat
     4081 tgattttttt aattaaattt gaagctcaac tcctttttta aacctttata tcattacaac
     4141 tttaatgttt atacgcctgt actttagatg tatatctact ttttacattt ctcaacatgc
     4201 aaacctacca ttttttgcag tatgttaatt gaaataaacc attgagttaa ccctaaattt
     4261 attctggact tataggaata gttccaaaga tactttaaaa tattttctgg aataattgct
     4321 tatttcacat atgttttatt gtgatgtttt gatttaataa attcttcaac tctgtatata
     4381 gtaaatgtat tatcatgttt gttttattca agcacactta taatacgctt gattttttca
     4441 caatgatgaa atatactaat gggtgttata ggtataagaa tcgcattaca cttgaagaag
     4501 taagtgataa atgagatata aatcctcata tgataaacta ctatttcatt gaggaacata
     4561 ttctaagtga tgtcaagata ctacaatatg actaaattcc tagtgataaa aaagtctacg
     4621 aatgagtgta gaaaggggaa ctaagatgaa taaagtttta attatagatg atgataaaga
     4681 tctttgtatt ttgttaaaaa agagtatttc aattgaacag attgaagcaa actattgcca
     4741 ttctgggaga gatggactgg ttgaactttc aaaacaagat tatcagttag tagttctaga
     4801 cgtgatgatg ccaggtatgg atggttttga aactatggaa caaatacgaa aatatagcag
     4861 tattcccatc ttaatgctga catcaaaaag cgataattac agcaaagtgc atggattgcg
     4921 agctggtgca gatgactatc taaccaaacc atttgaaatt acagaattta ctgctcgtgt
     4981 actgtcttta atccgacgtt acacccgttt taatccaaaa aatgaggagc cacagcaact
     5041 ttctttcaaa ggtttgttga ttgaccttga ccagcataca gttactacca atggcgaacc
     5101 tatgttgctt ccacccaagg aatttggtgt acttctttac tgtgcaaggc atcagggtaa
     5161 gattttgaca aaacagcaga tttatgaagc cgtgtggggt gagccatatg tatatgatga
     5221 cagcaatatc atggctatta tcagtcgcct aagaaaaaaa attgaactag acccatcatc
     5281 tcccatctat ttgcaaacaa taaaaggaat tgggtatcaa ttcaacacgg aggtatgaaa
     5341 atggatgaac aaacgataat tcttttctta ctcttgctgt cgattggtct actttttctt
     5401 tccatatttg ctttttccaa gcttgggcat atttacaagc gcctaaaaga tatagaggag
     5461 atactggctg atgtacaaat tggagacgaa aatcgtaaaa tactaattaa gccatgtgat
     5521 gttatggctc cattggttta tcagcttaat gagattgtct acgattatga aaataagtta
     5581 ctttctctga aaaagtcgga taaagctagc aaacaactta tgacaagttt atctcatgat
     5641 gttcgtacac cattaacaac tctgattggt tatctggatg ctgtacacag tggtatagtt
     5701 atcgaacaag agcgggagga ataccttgaa atcgctaggc gtagagccta cgacctcaag
     5761 gattacattg atgttctttt tgactggttt cggttgaact ctgatgaatt tacgctgtca
     5821 atagaatctg tggaaattgc agagttgtca cgcaatattt tgaaagactg gattcctata
     5881 ttccatgaaa agaaactgaa ttttgaaatt gatattccag aaaatcgact aatggtaaac
     5941 ctcgacccag atggatattc ccgtgtggtc aataatctgg ttcaaaatgt tcttgctcac
     6001 agtaaggctc ggcaaatcaa aattaccatg agtgaggatt ccagaatggt attactccgt
     6061 gttgaggatg atggtgttgg gattgccaga gaaaatttac cacatatttt tgagcgactg
     6121 tataagtgtg ataaaggacg ttcagaaaaa ggaagtggtc ttggtctatc tattgtcagt
     6181 caaatggtgg agcgaatggg tggtcaggtt tccgtagaaa gtgagattgg aaagtatacc
     6241 gtatttacag tgagcttacc attaatataa aattgttttt cccccatgtg acatgtatac
     6301 acagggggat tttttgtaag ttataaagtt gcaaggttaa tacaaggttg ttgcaaggtt
     6361 aatgtaagat tgaggtggta tgataacttt aataaacaaa ggaggctttt attatttatg
     6421 aataatttaa ttattgaaac aaaaaatctc actaagcaat atggtgctca aaagagcgtt
     6481 tctaatttga atattcacgt tcagaaaggt cgcatttacg gattgctagg aagaaacggt
     6541 gcgggtaaga cgacaactat gaaaatgtta ttgggtctaa caaaaccaac tactggagaa
     6601 attaaaatat ttggtcagga cattcacaat aacgagaaga agcttcttcc tcgaattgga
     6661 tgtttgattg aatcaccagg gttttatcct aatctgacag gaacagaaaa tctcaaaatc
     6721 tttgcaaggc ttagaggaat accaaaaaaa aatgcagtag aatcagcact ggatgtagtc
     6781 ggtttgccaa acaatgacaa aaagttattt tctcaatact ctcttggtat gaaacagagg
     6841 ttggcgattg ctcttgccat tatgcatgac ccacaactgt tgattttgga cgagcctatc
     6901 aatggacttg atcctattgg tattgcagaa gtaagaacat ttattcgtga tctctgcaca
     6961 gaacgaggta aaactatttt gatttccagt cacattcttt ctgaaattgc cttgttggca
     7021 gatgacattg gcattattga ccatggtatt ttgttggagg aagaaagtct tgcccaatta
     7081 gaagaaaaaa acagtaaata catacatttt gctgtatctg atgcagcaca ggcagcaagg
     7141 cttctggaaa cccaacttaa cctgaaaaat ttcaaagtag cagataatta caatattatg
     7201 ctatatgata cagatgtttc tgttgcagaa ataaatcgtg ctttcattct aaacaacatt
     7261 gaggtatcag aatctcattt atgcgaagat actcttgaag attacttcaa gaaagtaaca
     7321 gggggtgaag gaattgcttg atataatttc atgtgaattt tcaaaactca agcggttaaa
     7381 atttatactg atttctattt taggtgcttg cttatttcca attcccgcaa caattttgat
     7441 tgcaaaggat aacttgccct ttgagcaatt gttcaaatta gttgtcaatt ttggatattt
     7501 tttgctatta ccaattgtat taagtattgt tgctagccaa ctatttttta tagaaagaga
     7561 taatgatgta ttaaaaaatc ttgcaacagt gccagtgcca aaaggaaaac tggctctggc
     7621 aaaattggct gtgttgctgt ttatatcatt gttctactct gttgcagggt taggagctac
     7681 tatcattgga ggtttcattg ttggaatagt agagggggtt gctgtaaaat taggaatgag
     7741 cattgctttt ggaattatgt tttttgttac tgtacttcct gttgtggtac tcattgtata
     7801 tttcaatcgc agctatattt tttctattat actttctttt gtatatgcta ttttcaattt
     7861 tagtatatcc ttaaatatta tcaattttga accaaataac ccaatattaa gtgtattacc
     7921 tgctcctgtt ataatgcgtt ggtggatggc attttgggga gatcccactg gcgaatacac
     7981 ggcacttaga cagccatatt tgctctcaac tcctgcttgt gtaggtattt tatgtttgat
     8041 aaccattata gcagtcctgc tgatttgtat gatatacaaa aagcaggaga attaggaggt
     8101 atctatgctt gatataatta ctacagaatt tcaaaaaata aaaagatata atattctatg
     8161 gattggtgtt gttgctgttc tgttttcagc actattggca gtttttcagg ttgtaagttc
     8221 acacggcagt gaccctctaa cgtatgagag ctttgcaaac ggagttatct ggaataattt
     8281 cagtcttagt tttccatttg ggattacact tattggagga tatcttatta accgtgaata
     8341 tacagaccaa accttaaaaa acatcctaac tgttcctatt tcattacgca aattgctagt
     8401 gggaaaatta attgcagttg gaggaattac tgtattgtta agtcttttca gcttcttatg
     8461 cacatttatt ttggggttgt tattttgtaa tatcaatata tctgttacac tcgttgtaaa
     8521 atccttagtt cagattttgt ctgttaatct gtattgttat gttgctgttt tgccgattat
     8581 tgcttatttt ggtcgtaaac aaaatgcatt tctgacaggt gtagggattg catttgtata
     8641 cggtttttgt ggtgtgtttg ttgctggaag aaatctgaca gacttttatc caatcacagc
     8701 aggtcttggt ctagttggat acaacaatgg tgctggtact gtttatcagc cacttatcgg
     8761 tgctatgact ttgattgtaa ttctaatctt gacaactatt ctacttgtat ttacacctaa
     8821 ttatgataaa gttatgacca tatctaagaa aaaagggata aagcataaaa agtattctca
     8881 taaataacat aaatattgtt tctattattt aagttgctag acagtgaaaa gaaaaaatct
     8941 gtcgtacagt ggctatatgc tcatgtattc aagaagctac agttttgatt tttcataact
     9001 gtagcttctt tttgtattta ttaaatcacg actatcataa tacttgcaat aggtatagcg
     9061 acaagagaga gtactgtcat atgtgtttta tatattacat taagtatatt ctaaaatata
     9121 tgttttaaat aatatatttg aaaataggaa tatttaaata aaaaaaataa tattatatat
     9181 agttgacaaa aattggcaaa tgagatacta tataaatata gaaatagtaa tcattactat
     9241 tattaaaaag ggaggaatta attatgaact taaaaggaac taaaacagaa aagaatttaa
     9301 tggcaggatt tgcaggagag tcagaagcaa gaaataaata tacatactac gcttcaaaag
     9361 caagaaaaga aggttataat caaatagctg ctatatttga agaaactgct aacaatgaaa
     9421 aggaacatgc taaattatgg tttaaacttt tacatgatgg tatgccttca acagaggaaa
     9481 atttaaagga tgcagcagca ggtgaaaact atgaatggac tgatatgtat gcaaaatttg
     9541 ctaaagaagc tagagaagaa ggatttgaca agatagctta tttatttgaa gctgttggaa
     9601 aaatagagaa agagcatgaa gaaagatact taaagctttt agaaaactta aatgaaggaa
     9661 aaatatttaa aagagatgaa gaagttgttt ggcaatgtca aaactgtggt catgtgtatg
     9721 taggtactga agcaccagaa aaatgcccag tatgtgacca tccaaaagca tatttcaata
     9781 taaaagctga aaattattaa aaattatcat tattaaaaag gaatgttaac tatgaaattt
     9841 tctaaacaac gagaactgat tttaaatgaa atattaaata atccggttca tcctactgcg
     9901 gattacctat atgaaaactt aaaaaaagat aatccaaatt taagcttagg aactgtgtat
     9961 agaaatttag ctcagctaac ggaacatgga tttataagaa aggttagtat tccagggtat
    10021 ccagatagat ttgatggtcg aatagataat cactatcata tcatatgtga agtatgtgga
    10081 gaggtatacg atttagaatc cgaagttctt aataatttac aagaattaat atctgaggag
    10141 acagacataa aaataacatc ttataacata agttttaaag ggatttgtaa taattgtaaa
    10201 aggtgtagcc aagtaggtta gttttcaaat tctaaaaata attaatgagg tgagtttatg
    10261 tgtagtgaac aaaaattttt tatatgtgag acttgtggaa accttgtagg aatgatacaa
    10321 agtggtggtg tacctatttt ctgttgtgga aaacctatga aggaacttgt tcctaataca
    10381 acagatgcag cagttgaaaa acatgtacct gtaattgaag tggatggaaa taatgttact
    10441 gttaaagtaa gtagcacaac tcatccaatg actaaagaac atcatatagc atgggtgtat
    10501 cttatgactg aacaaggtgg tcaacgcaaa tgtctagcag tagatggaga acctgtagtt
    10561 aaatttgctt tgaatgatga tgataaagtg atttctgcct atgcttactg taatttacat
    10621 ggattatgga aggctgaact gtaatattta aataaattga gtcaaaaagg aggctataaa
    10681 tgtattttca tacaaatagc ctcctataaa tttatataaa ttcaagtttt aattttgttt
    10741 aatgtcaata taatataagg agaaaataat atgtcaattt ataaatgtag tgtttgtgga
    10801 tatatttatg atgaatctaa gaatgataaa acatgggatg aattaagtga agattgggaa
    10861 tgccctgtat gtacaaaagg tcgtagttat tttggaaaga taagtactgt ttactatgaa
    10921 gaagatgaaa agatagcaga agacatagtt gaagatgaat ctaaattaaa tactgaaaaa
    10981 gagggagatt taaattactt aagtacatat ttaagaagag atgatgaagt tgagaaacat
    11041 atggatataa ttcacgaaat ggctgttaca ggaaaatcta taatagaacc tatgagaact
    11101 aaactaccag taatttcttg ggatgatatt ttaattatgg gtgctcaatt aaatccatta
    11161 cctttaaatg agcatgatga agtaaataca acaacaataa taggaaaaaa agcaaagaaa
    11221 cctatgatta tagaaaatcc agtttatatt tcccacatgt catttggagc tttgtctaaa
    11281 gaactaaaaa tagctttagc aaagggtgca gcccaaaata agacagctat gtgtagtggt
    11341 gaaggaggta tattaccaga agagaaggaa gcttcataca agtatatttt tgagtatgta
    11401 ccaaataaat atagtgtaac tgaagaaaat cttaaaaatt ctgatgctat agagataaaa
    11461 attggtcaag gaacaaaacc tggtatggga ggtcatcttc ctggcgaaaa agtaactgaa
    11521 gaaatagcaa aagtaagaaa taagcctgta ggtcaagatg taataagtcc atcttgtttt
    11581 gaggaaatac aatccaaaga agatttaaag aaacttgtag atgaacttag agaagtttca
    11641 gaagggcgtc caataggtgt taaaatttca gcaggtcata ttgaaaaaga tatggaattt
    11701 atagcttatg ctaaacctga ttttgtaact attgatggtc gtggtggagc tactggagca
    11761 agtccaaagt tgcttaaaga tgcaacttca attccaacaa tatttgctct ttatcgtgct
    11821 agaaaataca tagatacaca tggattagat atagatttgg ttataacagg tgggcttcgt
    11881 atatctactg attttgctaa ggcaattgct atgggtgctg atgcagttgc tattgcaagt
    11941 tctgctctta tggcagcggc gtgtcaacaa tatcgtatat gtggttctgg taagtgtcct
    12001 gtaggagtag ctactcagga tgaggaattg agaaaaagat tacatataga aaacagtgct
    12061 aatagagtgg caaacttttt aaatgtaagt ttagaagaac ttaagacttt tgctagaatt
    12121 tctggtcata aagatataca tgatttaagt gttgatgatt tatatactgt taactctgaa
    12181 atatcaaatt atacaaacat acagcatgta tagattgaag tttgaaacta ttagatttta
    12241 gatagctata aaagtaaatg ataaatatat tttataaaag agagtgtctt aaaatgaact
    12301 ttttagttca taagtcactc tttttttgat gaagccaaat atatttatca ttttagcaat
    12361 gaaaaagaat cttatctctt tattttgaga tagactcctt ctatttatga aaattaaaga
    12421 tattaaattg taaaatattc tgtgatgtcg tactattaaa ctaaagattc tgtactttaa
    12481 agtaaataaa aatttaagga gtgattatta tggaaagatt gaagatacaa gttttagttg
    12541 acaataatac ttatattgat agatactttg ttggagaacc agcagtaagc tattatattg
    12601 agattgatgg aaataggatt ttgtttgact cagggtattc agatgtattc atatcaaacg
    12661 ctgaaaaatt aaatatagat ttaggaaatt taactcatgt tgttttttct catgggcata
    12721 atgaccatac aagaggtatc cagtttctag aaaataggta tgatttatct acagttgaac
    12781 ttatttcaca tcctaactgt tttataccta gaaaacatgg agagaaaagc ataggagctc
    12841 cattttctgc tgaggaaatt aaaaatattt ttatgtataa tcctaaggat aagccattta
    12901 atctaagtaa gaattgtgtg tttttaggtg aaataccttc tataaatgat tttgaaaaaa
    12961 gagctaaaat tggtaaatgt aaaataggag atttgtggga agatgattat gttttagatg
    13021 attctgctat agtttgtaaa actgataaag gtatttttat tgttacagga tgttctcata
    13081 gtggaatttg taatatagta gagtatgcta aaaaggtatg tggagatgat agagttattg
    13141 gtatactagg tggttttcat ctttttgaat tgaataatag gttagatagt acaatacaat
    13201 atttggataa agaagaaat
//
LOCUS       DAKBNN010000451         7569 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000451 DAKBNN010000000
VERSION     DAKBNN010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.188"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..734)
                     /locus_tag="OUB02_003975"
     CDS             complement(<1..734)
                     /locus_tag="OUB02_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCT8887643.1"
                     /translation="MIKVVYMRKNKKTRKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIK"
     gene            1095..1673
                     /locus_tag="OUB02_003976"
     CDS             1095..1673
                     /locus_tag="OUB02_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCT8887644.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            1666..2325
                     /locus_tag="OUB02_003977"
     CDS             1666..2325
                     /locus_tag="OUB02_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HCT8887645.1"
                     /translation="MNKQKFHKINLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(2536..4410)
                     /locus_tag="OUB02_003978"
     CDS             complement(2536..4410)
                     /locus_tag="OUB02_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCT8887646.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMIKDRETINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(4415..6079)
                     /locus_tag="OUB02_003979"
     CDS             complement(4415..6079)
                     /locus_tag="OUB02_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCT8887647.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(6093..6914)
                     /locus_tag="OUB02_003980"
     CDS             complement(6093..6914)
                     /locus_tag="OUB02_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCT8887648.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(7013..>7569)
                     /locus_tag="OUB02_003981"
     CDS             complement(7013..>7569)
                     /locus_tag="OUB02_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCT8887649.1"
                     /translation="RLNETEVEYKNQSKHQLMDCYNKVIDDIEKKDCSIMFERMKKAN
                     RIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIKTDDMIFFISLNG
                     DASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISISVDKQLDYDVTTP
                     YFILIELLYIKYKMYLESEHIPRK"
BASE COUNT         2613 a         1317 c          813 g         2826 t
ORIGIN      
        1 tcttttattt tttcaatagt ttttataggt accattgaat tttcttttac ccaagatgca
       61 tttaatttct tttcaatttg ttcaactgat acattcaata aagtagataa tttttttaag
      121 tcttctatat tatcactcat ttttccagga actaatccta tcatagatgc aagaccttga
      181 ccagcaatca ttacaccgtt cctatccagg atacgtcctc tcttaccttt cagctttgat
      241 acccttacag tattatcttc ttcaagttct ggaaatatga cgtttgactg ccattttatt
      301 ttataatttt tctgtctatc tctagttaat agtacttcat ttgaaaatga aatttcacct
      361 gctaaagtat ccatcttaga atcatagttt attactttat cattattttt atttttttta
      421 atatcattaa tcttaatact tatatcgtgt gcatcaattc cttcatagat ttttttattt
      481 cttaaaacaa aatcttcttt tttattttca gacttgctct tttcatctaa taaatcatac
      541 attttctcat attgtttttt atttgcaaaa gacatatact gcttaaacac ttcatctggt
      601 ttgtttttat tgagtccatc tagtatgaat tttgctaaaa aaacaataat aactataagc
      661 agacacatta gaaaaccaaa aattatcatt tttcttgttt ttttattttt tctcatataa
      721 accaccttta tcatttaata tattacatac gtaagcttta aggttaaaat actcttatag
      781 acataactaa tctatgtttt tattatgttt ttaagtatct cataaaaata tgtaagcagt
      841 ataattgata gtaacttata tattacacat gtaagtttta aaagtcaata atattatcat
      901 tatttaatta ttttttacat caacttaaaa ataaatcagt tccattatat tcttttgtat
      961 tcttcaacta ttttataaat taaccttatt ctattgtaat tattttatag tatataactt
     1021 taacttgaca aaacgacaca agtgtctcat aattaaaagt gacacttatg tcgttaaaaa
     1081 ggaggttaaa acatatgcca acaaaaggag aacaaactaa acaatttatc attgatacat
     1141 caataatgct attttctgaa aaaggatata gtaatgtgac aatgaaagat atatgtgaag
     1201 catgtaattt aagccgtggt ggcctatata gacactttaa ttcaccaaaa gaaattttta
     1261 tcagtatatt aaacactcat aaatactact ctcaaaattt gttggataaa ggtatcaagg
     1321 aaaatatatc agctaagcat atgctagaat attttttcca acaacaaaag gctgacataa
     1381 atggcaaata ttgtggattt gattttgcaa tacacgaatt tgcttttgta gaaaaagacc
     1441 aacgtgatta tatgaataaa agatacagtt cagctatcac aatgctttca aatttaatcg
     1501 attatggaca atctaaaagt gaatttaaaa aatgtgattc taaatcagtt gcaatacaca
     1561 taatgttctt atttgatagc atgaaaacat ctgcttccat tttgaaactt acaaataaag
     1621 acattgataa tcaaataaat attataaagg acatggtgat tttatatgaa taaacaaaaa
     1681 ttccataaaa ttaatttaca aaactgggaa cgatatacac atttttacta cttttcaaaa
     1741 atgacaaatg tagggtttaa tttgactata gaaatagata ttactaattt gcttaaggaa
     1801 acgaaaaaaa gaaatattaa attttatcca gcgtatttat atacaataac tacttgctta
     1861 aatcgatata gagaactaaa aatcgcatat gatgaagatg aactcggata ttggaacacg
     1921 ttaacgccac tttatgcgat ttttcatgat gataataaga ctttctcatc aatatggaca
     1981 gaatatgatg aaaatttctc aatattctat gaaaattatt tagctgatgt taaagaatat
     2041 ggaaaaaatc atggttttct agcaaaacca aatccgccaa gaaactgcta tactgtttcc
     2101 tgccttccat ggattagctt taagagtttt actgtacata atcatggtct tgaaaattat
     2161 tttttcccta caattgaagc aggtaaatac tatcaaaaag atgaaaaaat acttcttcct
     2221 ctttctataa cagtgcatca cgcaactact gatggatacc atgtaagttg ttttattgat
     2281 tacatacaaa aaatgtgctt taatactaag atatggctac actaaaaatt tatacaatat
     2341 aaaaagaact gtcgcatgag caaattaaat ttgctatgag aaagctcttt tttacttaca
     2401 atagttaata tgagatgaat tatttcttat actttgaact taccaatagt taatatgaaa
     2461 tgaattattt ctcatacttt aaaaagattg aattactctt gataatcttc ttttgtagtc
     2521 attacatatc acctactact caacacatat tctttgacct atttttacat ccttgtttat
     2581 atccaaaata tttaccttct taccatctgg aaacaatacc attgttaaag tagactttcc
     2641 taattcgtgt attctcttta aatcaacatt aacaagaacg tctccctgtt taaccatatc
     2701 tccttgttta acagcaacat caaagccatc tccatttaat gcaactgtat caattccaac
     2761 atgaattaat acttctccat tagttccttt aatcccaaat gcatgtccac ttgaaaatac
     2821 tgtttctaat ttacctgaaa taggagaaac aatttttcca tctataatat caatagcaaa
     2881 accatcacct aacatttttt tagaaaatgt ttcatctggt acattttcta atgcaacaat
     2941 tttcccctta gctggagaca caaagttttc attattttca ttaaacacta attcttcgtc
     3001 ttcatcttca tttatagctt ctttaggcac tccaaataaa catgttacaa taaagcagaa
     3061 tgcaaccaca aatattgata ctaataagta tgtgattaaa ttatatccat catagatata
     3121 cattaaataa gaaggtaaaa ctgctaatcc atatgaatta gattgaatat gtaaaattga
     3181 taatattgct gctcctaaac ctgaagatgc acacataata atcaaaggct ttaaattata
     3241 tcgaagctga atcccaaata aaacaggctc acttatacca aaaagttgag atagcattgc
     3301 tccaactgca gtttgtttaa cttgattatt tttagatttt ttcataaatg ctagacatgt
     3361 tccaatattc gcaaatccat acattgcaca aagagtaata agggcattaa atcctgtatt
     3421 tgctaatagt gatgtttcaa tcattacata agtatgatgt aagccagtaa taacagctaa
     3481 tggatatgta aatccaataa taaatcctcc aattcctaaa ggcaaatcaa tcaataagct
     3541 gatgatatca accattttta attctattgt atgcattata gggcctacac ctaataaaat
     3601 aactaacatt gttattagca taacaaagaa tggtgtgaaa ataaggtcta atacattagg
     3661 catatgttta cggaaccatt tttcaagatt agctccaata aatgctgtaa caatagctgt
     3721 taaaacactt ccttgacaac ctacaatagg aataaatcca aatgccataa ttgcttcaat
     3781 ttcatttgat gggtcagcaa cagaatatgc attaggaagt attggggaaa ctaacataag
     3841 tccaataaca agtccaataa caggtgttcc tccaaatact ttaaatgctg accatacaat
     3901 aattgctggt aaaaaagcaa atgctgtttc tgtaagaacc gaaacaagtg tctgaatata
     3961 caatggaata tttgcagaag acattccaaa taatccaagt acattatcat taaacaaaca
     4021 accctttaat cccaagaata atcctgttgc tgcaataact ggaataatag gaacaaatat
     4081 atctgctaga gttctcataa ctttcttaaa tccagtttca ttatttttaa ctaattcatc
     4141 ttgttctttt tttgataaag tttttaatcc cattttttca acttctgcat aaactttatt
     4201 aactattcct gttcctaaaa taatttgata ctgtccagaa gtaaagaaaa cacctttaac
     4261 ctcattgacc ttttccactt tcttatcatt aatagtttcc ctatctttaa tcatcaccct
     4321 tagtcttgtt gcacaatgtg taatagaaat aatattatct tcaccaataa ttttaataag
     4381 ctcatttgct attttattat atttattcat ttttctacct cgctttcaat aatactgcat
     4441 gaaatggttt caaacacact gttttattta atagatttat atcatctaaa ttttggaata
     4501 caatatcata tttttcttct actcttattt ctacaggttt atcagaaaaa ttataataac
     4561 aatcaataac taccaaatct aattgccttc tatacgcaat aacatcttca ctttctatat
     4621 caataggttc tattgaacca taaatcaaac aatcagaata ctcactatct tgtctcaact
     4681 ttatcatctt tttataaaat tctaaaatac tctctgaatt gttaatctga ctctgtgcat
     4741 taatatcttt ataattttca ataaccttta accatggtgt tgtatctgaa aaacctgcat
     4801 tttctttatc tgcccatgga aatggcgttc ttgcattatc tcggcttctt aaattcacaa
     4861 attctaacgc ttcttcttct gagtaacctt catctattga acgatagtat tggtcaatgc
     4921 tagataaatc attaaaatca tttatggtat ctcttttaaa atttgtcatt cctaattcct
     4981 gcccctgata aataaatggg gttcctctta aaaagaaata cattgctccc aacattttta
     5041 ctgcatcatc attcctttcg tattgcaaaa gatactttgt tgtagctctt ggttggtcat
     5101 gattttcaat aaaattagct gcccatccat gttcctgaac atacatttga ctatctaaaa
     5161 tgcattttct taaatctgga attgtccatg aaattctttt aaaccactca ctaccagaag
     5221 caacatctaa gtcagcatgt ttaaagtcaa agaccattga aaagaaacca tcttctccaa
     5281 taaaatcacc taactggtca tagctaatac ctgctgcttc agcaacagtc atacaatcat
     5341 attttttaaa cgtattttct tttaattcat aaagcatttt ttctattcca ggctgatttc
     5401 taactgtttt tgtacattta acaagaccat caacaccatc actttctaaa ctttgaaaac
     5461 ttaaattctt tttgatgaat gtaatagcat caactctaaa tcctgccact cccatttgag
     5521 accaggtatt aatcatttgg taaagttcac ttcttaatat tggattttcc caattaaggt
     5581 caggttgttt tttaccaaaa gaatgaaaat aatattcgtc tctattctct accttttccc
     5641 aaacacttcc accaaaaaca gacctccagt tatttggtaa atcgtttcct tttttaaaaa
     5701 tataataatc atgataagga ctttctggat tttttaatgc ttcttgaaac caaggatgtt
     5761 catcacttgt atggttaaga actaaatcta atataatttt gattcctcta tctttacttt
     5821 ctttaattaa ctcttttaaa tcttccatac ttccaaattc acttgcaata tccaaataat
     5881 cactaatatc atacccatta tcgtccatag gtgatttaaa aattggacat aaccacaatg
     5941 atgtaatacc taaatctttt aaataatcta aacgagaaat aattccctgt aaatctccaa
     6001 ttccatcatt attactatct tgaaagcttc ttggataaat ttgataaacg atttcttttt
     6061 gccaccattt cttttccata aattactcct ttttatatga tatttcttct ttttaattct
     6121 ttataaattc cattatcgaa tatgtcctca catatagatg tcgctatatc tttaagttgt
     6181 tgatgactat ttttcattgc aattgtaacg tctgatgctt gaaacatgac aatgtcattt
     6241 tgaccatcac caaaacatat ggtttctttt tgagttactc ctaatctttc ttgtaatctc
     6301 ttaattgctt tgcccttatg aaaatctttt tgaataattt cataatactg actagaaata
     6361 tctctctgtg ctaattccat tttatcttgt agtatatctt tcacttcgtc aaataccttt
     6421 tcatttgacc ataaacaaat tttatgtata tcttgtgact tatactcttc aatgttattt
     6481 tcatatgtta ttttttcttg gatatgttgt ttatttatac aagaattagt tcctttcaac
     6541 tgattcattg tttcaaatat ttctttggct ttttgattca tgaaaacttt ttcctgactc
     6601 tcaatcgaaa aagcaacttc cctctttttt aataaacaaa caacttcctt tatcaatctt
     6661 tgattaaaag attgattata aagtaattca ccatgatatt gaatgtaatt acctcctcct
     6721 gcaatatatc catcaactcc taatgataaa acatcatctt gtattgtacc catacttctt
     6781 ccagtacaaa taacaacact acaatgattc ttttgacata aacgtatagc atgctttgca
     6841 gattctggga ttccatatac ctcatctcta agcgtaccat ctatatcaag tattaataat
     6901 ttgtacaatt tcatcttcct ttcttactat caaaatcata atagcattca aaaaaaaaga
     6961 tatcaatatt ttgatatctt ttaaaaaata tgttcatttt tagcacaaat tttcattttc
     7021 ggggaatatg ttcactttct aaatacatct tgtatttaat ataaagcagt tcaattaaaa
     7081 taaaataagg tgttgttaca tcgtagtcca gttgtttatc tacagaaata gatatagatt
     7141 gaatatacag gttttcatca cataatcttg ctaaaggatt acttatcatt tttgtaatag
     7201 aaaccatata aattcctttc attctcattt ctttagcaaa atcaataatt gtagatgcat
     7261 ctccattcaa tgaaataaag aaaatcatat catctgtttt tataaaatga ttaagtgcct
     7321 gaatcatatc ataaccataa acatcatata taattttccc tgttggtaag aaaatccttt
     7381 tcatctcttt agcaactctt tcctgagaat aaccttctcc aaagacgatt atacgatttg
     7441 cctttttcat tctttcaaac ataatcgaac aatccttctt ctctatatca tctatcacct
     7501 tattataaca atccattaat tggtgtttac tttgattttt atattctact tctgtttcat
     7561 ttaagcgta
//
LOCUS       DAKBNN010000452         7526 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.190, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000452 DAKBNN010000000
VERSION     DAKBNN010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.190"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            83..1036
                     /gene="blaCDD"
                     /locus_tag="OUB02_003982"
     CDS             83..1036
                     /gene="blaCDD"
                     /locus_tag="OUB02_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HCT8887650.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKCVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1380..2063
                     /locus_tag="OUB02_003983"
     CDS             1380..2063
                     /locus_tag="OUB02_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887651.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTYGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDFLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2060..4720
                     /locus_tag="OUB02_003984"
     CDS             2060..4720
                     /locus_tag="OUB02_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887652.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWDSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKISASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(4901..6082)
                     /locus_tag="OUB02_003985"
     CDS             complement(4901..6082)
                     /locus_tag="OUB02_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8887653.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTLWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGAKFIIKFY
                     T"
     gene            complement(6082..6744)
                     /locus_tag="OUB02_003986"
     CDS             complement(6082..6744)
                     /locus_tag="OUB02_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887654.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(6882..7520)
                     /locus_tag="OUB02_003987"
     CDS             complement(6882..7520)
                     /locus_tag="OUB02_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCT8887655.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVIGKSYLNNFNIDEEKFIETTLALLIDSIKA"
BASE COUNT         2786 a          964 c         1172 g         2604 t
ORIGIN      
        1 acctagattg gagtatatta caaataatgt ctactatttt aatagtaggt tataaattta
       61 aatatgggag atgatataaa ttatgaaaag aaaaaagaat tttatttgga tagctatctt
      121 attggttggt gtagttatgg taatgtatta tgtagggaaa aaacataacg atataaatca
      181 aaaaactgat aagaattata taaaatctga attaaataag tctgaattaa ataaatccaa
      241 aattgaaaac aataataaaa acaaaaaaga aaaaattaat atggttgact atagtgattg
      301 ttttgaaggt attagtggag gagctatttt ttataatact aaaaataaag aatataatat
      361 atataacaaa gaattaattg aaacaagaag gtcaccatgt tctactttta aaatagtttc
      421 aactttaatt ggtttagaaa aaggggtaat aaactcaaaa gaatcagtta tgggatatga
      481 tggtacagag tatccaaata aaaattggaa taaaaatctt agtttagaag aggcatttaa
      541 ggagtcttgt gtttggtact acaaaaaatt gatagataaa gttgatgcta aatctgtcca
      601 aaacatttta gatgatttaa agtatgggaa ttgcgatatt agcgaatggg aaggagattt
      661 aaaaaatgga aagggacatt taaatggatt ttggttagaa tcttcattac aaatatctcc
      721 aaaagaacaa gtgcaaacaa tggcaaaaat atttgaggga gatacaaact ttaaaaaaga
      781 gcatattaat attttaagag atattatgaa aatagatgta aatgataaaa acataaatgt
      841 atatggaaaa actggaacag gatttgatga aaaaaataaa tgtgtggatg catggtttgt
      901 tggtatgcta gagcgtgaag gtgacaccta ttattttgct attaaatcag atgattccaa
      961 taaagaaatt acagggccaa aggtaaagga aattgcaatt aatataatta agaaatatta
     1021 tagtgtaagg gaataaactt aattttattt gtaaatatta tttacctata gtctgtaata
     1081 tttattaaat aattttacta aattgagtct atatagagca aaactccttt agtacataaa
     1141 atacttgcta ttgattatta aattgaatac ctatagattt aaagttaata ccttttaaag
     1201 actatgtaat atattaaaag tataaataaa atatattgca tagtttttgt tttatattat
     1261 aattttaaat aatgtgcaaa aactaaacaa tatttttaat agtaatttat atttgtattc
     1321 atgtcactat aatgtcattt taggttgcta aaatttaacc ataaaggaga tgattgtata
     1381 tggaaatatt aaaatgtgaa aatctcacaa aaatatatgg ttcaaatcaa accagagtga
     1441 cagcccttaa taatgtaaat ttatcagttc aaaaaggtga ttttgtatcc attgtaggag
     1501 catctggttc tggcaaatct acattgctac atctgcttgg gggagtagat agacctacat
     1561 atggaaagat ttatgttgaa gatacagaaa tatcaagttt aaaggaagaa gctcttgcag
     1621 tttttagacg tagaaaagta ggtcttattt atcaatttta taatcttata cctactcttg
     1681 atgttagaaa aaatatatta ttacccatgt tacttgataa aagaaaggtg gatgaggata
     1741 gattctctga gattgtatct atattgggat tatctgatag attaaaccat cttccaagtc
     1801 aactttcagg tggacaacaa caaagggtat ctatagctag aagtttaatt tatagaccag
     1861 ctattttatt agcagatgag ccaactggta atctcgatag aaaaaattca gaagaaattg
     1921 tagatttctt aaacttatca aataaacgat ttaatcagac aatacttctt attactcatg
     1981 atgagaaaat agcactagag gcaaatcgta ttgtaactat ggaagatgga gtgattgtct
     2041 ctgaaaaggt ggtgaagaaa tgaatattct tcaagaatac accttagact ttgtacgacg
     2101 caataaacgt agtagtatag ctataatgtt agctatatta cttactacaa ccatgatgtc
     2161 ttctttgtgt ggactgttgt ataccatgtg gacagatttt ataagacttt caattgagaa
     2221 agaaggtaac tggcatggtg aattgtttga ctatacttat ggaagtgact taccattaat
     2281 tgaaaatttt tcttcagttg atgatgtaat ggtaaaagga caatggtatg taggcaaaat
     2341 taatgatgag aaaagagatt atataattta tagaaatgct acatcagagt attgggattc
     2401 tatgccagaa aaggatacta taatagaggg tcgtcgacca aataatgcta atgaaattgc
     2461 attatcaaaa caatattttg agaataatcc aagtgtaaaa attggtgata aaattactct
     2521 cccaatagga gatagaatcc taaatggaaa gcctcttgaa cccatagccc caaaatcgaa
     2581 aggggaaagt tttaaaaaaa tttcagagac tacattgaca atagtaggaa aggtagatgt
     2641 ttctacttca tcagtaatac ctgcatatac agctcttgga tatcttgata aaacaagtat
     2701 aaaacctaat gatagtataa ctgtgtactt gcgatttaaa aatataagag acacgtataa
     2761 ggaacttcca aaacttgcta aatcattggg gtggaagact aatgaatatg gaaaatacaa
     2821 tcttaaatac aatagtaatt atcttttaaa gatgttagtc ttttcaccag aacagaaagc
     2881 atctatgagt agtatatcaa aattttcaac tcctattatg tatcttacaa tagcaatttt
     2941 tacagtagcc gtgtttgtaa tggtgattta caacgcgttt tcactgtctg caaatgctag
     3001 acttactcag ttagggatat tatctagtgt tggtgcatca ccaaaacaaa ttaaaaggtc
     3061 tgtagtgttt gaaggatttt tactgacaat tataccatta ccaataggtt tatttttagg
     3121 atggttattg tgtaataggc ttatagttta tgttaattca gttaattatc atactgatgt
     3181 tccagaagtg gtatttactt atggtattcc tgcttttctg ccagcagttt tacttacaat
     3241 agtaacagtc tggatttctg cacttatacc agcacgaaaa gtatcaaaga tatctcctat
     3301 agaagcaata cgacaaggag atagtgttaa aatcaaaaag tcgcataaat attcattagg
     3361 aaagatattt ggaatagaag gagaacttgc atcaaacgca ctacgtgctc gtaaaaaatc
     3421 ttatcgtact gcaacaatat ctttaacatt atctttttta ttacttacat gttttttgca
     3481 tataattgca aatcaagaag gagcaaaagc tgtttatgga agtgataatt ataaggacca
     3541 aagagatata gcagtttatt tggaaaatag tgaacctgta gagtctgctt tcattgaaaa
     3601 attagataat acagaaggtg taaaaagttc actatattat caaaaaatat ctgcttccac
     3661 ttggctgact gaaaaagatg catcaaaaga acttgcgaaa aatggaggat ttaaaagtat
     3721 agtttcatct aaaaaatact ctcctataga acgtgatggc aagtttcgta taatttcaaa
     3781 tataattggg cttgatgata agagttttac agaatattgt aggcaaattt ctgtagaccc
     3841 aaagttattt tatgatacaa aaaatccaac ctcaattgta tataataagg aggaagatat
     3901 taatcgtagt acacgtagaa ataaagtata tatagattat ttaaatcttt caataggaga
     3961 taacattaaa ttaaatgaaa aagtatatga cgaagataaa ggggattata cttacaacat
     4021 gaaagtggga gctattacaa gcacacttcc taaaatagta gatactagtt caaaatatac
     4081 attaatgcaa atagtgccaa tgtctatggt gcaggaaata tctaaaaatt atgatgaagt
     4141 aagtaggtta cgttcaaata ggctgatagg agtatttaaa gtaaataatc gtgaggacat
     4201 tcctagagta agagaaaaat tagagtccat ttataataaa gactatggtt caggagatta
     4261 tagtattgag gatgttttag aaagtgaaaa acaaaaggct agtggaataa agaccatgaa
     4321 tttaatagta ggctttatta caggtttatt ggctatgatt ggattatcaa atgtacttgc
     4381 tacagtgtca ggaaatatta gaagtagacg acaagagttt gctatgttgc gttctgttgg
     4441 attatctcca gaaggcataa aaaagatgct tattttggaa ggtttattct taggaattac
     4501 accattacta cttagtattc ctgtgcaaat aggaatagtg tatgcttttc tacgtataaa
     4561 tgaaatatat tttatagagt atttaccatt tgcacctata tctacaataa taggttttac
     4621 tatattgata ctttttattg taatagcatc atatatgaca ggatacaagc agttgaagaa
     4681 tgaaaatata gtagaatcta taaaaaatga aacaatataa acaaaatcta tttgaactta
     4741 tttaaaggtt tcgatttaag actacttaca agtataggga tagttaattt gtttgtttaa
     4801 taaaacctta aatgtaaata gaagtatatt actaaaacaa tagataacat agaatataga
     4861 tatagctgga ttataggtat tttacctata atccagcatt ttatgtgtaa aatttaatta
     4921 tgaattttgc accaccatta tggcaatttt ctgcatgtag agtaccattt tgacgttcta
     4981 taatagactt tgatagagca agccctatac caatactgtc cttactagca tttttaccac
     5041 gataaaatct attaaaaata tatggaagtt cttcctgagc aaacccctta ccattatctt
     5101 ctatgataat ctcaacatat agaggatttt gactataata agcaactata tatccaccat
     5161 cttctatatg ctcactacaa tttttaagaa tatttaaaag agcttctaaa gtccaattaa
     5221 tatcgatttc aaattctacc gaaggagaag cctcgatagt aaatgtctga tgtttttctc
     5281 ttaaagaacc ttctataggc tcaattgcat aagtaagaag agaatgtaca tctataagtt
     5341 tgctttcaaa tattaaagta cttgcatcca attttgataa tgtaagtaaa gatgaaacta
     5401 gtctctcaag tcttgagatt tgatttgtaa gcctgttaat ataatctatt tcttcatctg
     5461 gacaattttc tgaaagtaat tgtgccataa gtgacataga agtaattgga gtcttaagtt
     5521 gatgagcaat atctgcaaga ttatctgaaa gagtttgtct ttcttttaaa gcaaattctt
     5581 ttgaacaacg aagttcccca actgttttat atatctcatc ttcaaggtga gaaaattcat
     5641 cttcacatcg taaaagtaca gactcttttc ctaaattcac ttcttctaag tattgagtaa
     5701 gattgtttat tctactcagt ttttgcttat ttgttctatg tttgattata tatacaactg
     5761 tagccattgt tgaaattata ccaaacgata tcactagaaa ataaaaaaag tttttactcc
     5821 aaagtgtact tttagaatat ccatgttttg ataaaatttc atttcctgtt ttaaaatcct
     5881 tatcactaga atttttcagt gattttagta tactttctgt agacttagga ttgtactcat
     5941 atgatgcacc aagagcctta gacagtacat tgtattcatt aaaagaagtt atagttgtta
     6001 gaattacact caataaaaaa gttacaatta agcagaatat tataaaaaca actgtactgt
     6061 tgatatttct tggagttttc actagttgtc ctccattcta tatcctatgc ctcttaatgt
     6121 ttttatacaa ggtggatttt ttaatttttc tctaagacgt tttattgtaa cagtaagagt
     6181 gttatcattt acgaaattac catctacatc ccagagcatt tctagtaact ttgtacgagt
     6241 catagctcta tttttatttt ccataagact tagaagaagt ttatattctc ctgctgtcaa
     6301 aggtacttca acattatcta aatatgcttt gacttcaacc ttattcaagc ttatattttc
     6361 acatgtaata gtattgttag tatttgaatg agttcttcta agaactgcat taattctgga
     6421 ttgaagtact gagagcaaaa acggtttaac cacatagtca tctgcaccca tatctaatcc
     6481 acgtataatg tctttttcat catcacggac tgtcaaaaat atcactggag tatcttctat
     6541 acttttaaca tattggcaaa actcgtatcc aataccatca ggtagattta aatcaagcag
     6601 aattaaatca atagttttat caaaattttt tttagcttca gcaatcgtat tataacaaca
     6661 tacagtgtga tttttctttt caagggaagt ttttattcca aatgtaattg tatcatcatc
     6721 ttctaaaaga agtattttag ccatttactt ttaatccttt cagtaattta gttattccat
     6781 taactaagtt attagtttta tgtttaatat tttttatatt agagatagaa aaaattatca
     6841 tttaaattta agtatataaa ctttaattta taaacttagg tttatgcttt aattgaatct
     6901 attaaaagag caagagttgt ttcaataaat ttttcctcat ctatattaaa gttgttcagg
     6961 tatgattttc ctattacaga tagtaaatta aaaaatcctg taagtgtaaa tattgatgaa
     7021 tacataagag taggtatttc aatatcatgc ctaatacttc tatcattttt tccaatttca
     7081 aacattcctt gtatttcatc aaacataaat ttattaaaag aaaaaaactt ttctttaaac
     7141 agcatatcag tatccgattt ttcagagttt acaataccaa tcattcccat aaggcataat
     7201 agagaaggtt ctacagagta aaatttatga aaagcaaagt atgaaagttt tattttttca
     7261 aaccctgttt tgccttttgc actttctatt ttaacattat cccaaagtct tttatatcct
     7321 cttaagacaa cagcaaagaa taaatcttct ttacatgaaa agtacttgta tatggtgcgt
     7381 ttagtatatt caacttcttt tgctaattca ttcatagttg tattatcaaa tccattaaga
     7441 caaaataatt tttctgcctt atcaataatc tcattttctc tttgtatttt ttctttatct
     7501 ctttttgata attctgacat ataaat
//
LOCUS       DAKBNN010000453         6946 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.200, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000453 DAKBNN010000000
VERSION     DAKBNN010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.200"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            <1..173
                     /locus_tag="OUB02_003988"
     CDS             <1..173
                     /locus_tag="OUB02_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887656.1"
                     /translation="EISSKDEGQRIYKKLFMKDGSLVGAILINDLSCTVKLIRLISEK
                     GDFEDIMKADIL"
     gene            607..1308
                     /locus_tag="OUB02_003989"
     CDS             607..1308
                     /locus_tag="OUB02_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCT8887657.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1358..2440
                     /locus_tag="OUB02_003990"
     CDS             1358..2440
                     /locus_tag="OUB02_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCT8887658.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2701..3801
                     /gene="vanG"
                     /locus_tag="OUB02_003991"
     CDS             2701..3801
                     /gene="vanG"
                     /locus_tag="OUB02_003991"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HCT8887659.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3798..4604
                     /locus_tag="OUB02_003992"
     CDS             3798..4604
                     /locus_tag="OUB02_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCT8887660.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4622..6760
                     /gene="vanT"
                     /locus_tag="OUB02_003993"
     CDS             4622..6760
                     /gene="vanT"
                     /locus_tag="OUB02_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HCT8887661.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGVKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         2553 a          767 c         1200 g         2426 t
ORIGIN      
        1 aagaaatttc atcaaaagat gaaggtcaaa gaatatataa aaaattattt atgaaagatg
       61 gttcattagt tggagctata cttataaatg atttaagctg tactgttaaa ttgataagat
      121 taatatctga aaaaggtgat tttgaggata ttatgaaggc tgatatacta taatttaaat
      181 acattaaaat aaaaaggagt tgtctcaaaa ttgatttttt tctaatcaaa ttttaaattc
      241 aactcctttt ttgaattttt atataaaata acatggaaat tttagtaatg tctgtggtta
      301 catacatagg tttaattatt ttttatatgg attgtctctg gtataataaa ttgaattgtg
      361 tttgactgat agatattttt attgaaagtt aatacctgtt atgcgataat aaggacgtgt
      421 taggggagtt tttataataa caattaatct cagtttttgt gttccaatgg tagcatcaac
      481 acattctgat gtagtagttc ttttcttata aaaattagtt acaaatatgc ttgtaattat
      541 ttgatatgta aatttaccaa tattataaat gatgacctcg ataataagga gagtgaaaaa
      601 taatctatga acgaaaatat tttgattgtg gatgatgaaa aagaaatagc agatttaatt
      661 gaagtatatt taaaaaatga aggttttaca gtatacaagt tttatacagg taaggaagct
      721 ctagaatgta ttgaatctaa aaatttggat atggctattt tagatattat gcttcctgat
      781 attgatggat ttcatatttg ccagaaaatt cgagaaaaat actattatcc tatcattatg
      841 ttaacagcta aaattgaaga tatagacaag ataatgggtt taaccatagg tgctgatgat
      901 tatataacaa agccatttaa ccctttggaa gttgttgcaa gagtaaagac acaattaaga
      961 agatatatgc gatataataa ttcttatgaa caacagtcga taattgttaa tgagtatgat
     1021 attaaaggat tgattatcaa taaagaaaca cataaatgta gtttatttgg gaaagaagta
     1081 gcattaacac cgattgaatt ttctattctt tggtatttat gtgaacatca aggaaaagtg
     1141 gttccttctg aagaattgtt tgaagctgta tggggagaaa aatatcttga taataataac
     1201 acagttatgg ctcatatagg gcgattacgt gaaaaaattc atgaaccttc taaaaacccg
     1261 aaatttataa aaactgtatg gggagtaggt tatacaattg aaaagtaaaa atattaaagt
     1321 caatcctgac tttactcaag tgagaaataa aatttttatg agaacagctt taatggcatt
     1381 tatagccatt attttcatat ggattttata ttcttttata ttgtatggta agttttctat
     1441 ctggatagta tcattttttg aaaatgcttt tcgtatggaa tataaagctg cattgaattt
     1501 atatcagcag attttccgta atagcatgga gctcttcatg atactagcta tggtgattgt
     1561 gttttttata atattccgtg tttatttaaa ttggtttaca aaatatttta gtgaaataaa
     1621 tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat ctccagagtt
     1681 attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac gtaaatttga
     1741 gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac atgatttaaa
     1801 aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa gtcagatttc
     1861 tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc gtcttgaaga
     1921 tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat cacttgaata
     1981 cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta aacctacttt
     2041 gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga taaggtgtga
     2101 tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt attatagttt
     2161 tgaagatact actattgaaa taacagctgt acaggaggat gattatataa agcttaaatt
     2221 tatgaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg aacaatttta
     2281 caggttggat acatctcgta attcaaacag tggtagagct gggttgggac ttgctatagc
     2341 taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga acaatataat
     2401 tacctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt ataaaaaaat
     2461 aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata attactgtat
     2521 caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa tgtgaaaata
     2581 tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa tagaaaaagt
     2641 attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg agaatcaaag
     2701 atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata tgatgtatca
     2761 ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga tattatacca
     2821 attggaatta caagggaggg taattggtat cattatattg gagattataa taatatccag
     2881 aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc gcagaaccgt
     2941 gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa tcatattcca
     3001 aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga ttttgtgttt
     3061 cctgtattgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt tgaattggca
     3121 ggtatacagg ttattggatg tggtactctt tcttcagcat tatgtatgga caaagacaaa
     3181 tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt actaaaatac
     3241 tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt atttataaaa
     3301 cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga agagttacaa
     3361 gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga aaatattgat
     3421 ggttttgagg tagggtgtgc agttttagga aacaatgact tacttgttgg aagagtagat
     3481 gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt gaaaagttca
     3541 aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca agagacagca
     3601 aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat gtttttaact
     3661 ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac ttctcatagt
     3721 cgttatccaa atatgatgaa aggtattgga ctgtcttttg aagaaatatt ggacaagata
     3781 attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa gaagataaaa
     3841 tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta attctagtaa
     3901 atgctttttt accagttaaa acatctgaag atatagattt aattcctgta gatacaaggt
     3961 ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt ttaaaaagta
     4021 tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag gaacaacaag
     4081 atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag tttgtagcac
     4141 ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta aagaaagaag
     4201 ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag tttcgtaagg
     4261 cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa ataacaggaa
     4321 tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag attatcaata
     4381 aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg tatacaaatt
     4441 gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct attcatacta
     4501 aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg ggtaacaata
     4561 aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg gaggaacaag
     4621 aatgattagg aataaatctt atgcagggct tgattatttt aggattatag ctgcattact
     4681 tataattgca attcatacat ctccattatt atcatatagt aaaatggctg actttgtgtt
     4741 aactagtata attgctcgta ttgctgtgcc attctttttt atgctatcag gcttttttct
     4801 aatttcaaca tataattata attgtaataa actgaaatct tttctaaaaa atgtattatt
     4861 tatttacggg atagctattg taatttatat tcccttaaat atttacactg gatatttttc
     4921 catgaataat ttagtgccca atattattaa ggacattgta tttgatggga ctttatatca
     4981 tttatggtat cttcctgcat caattattgg tgcaagtatt gcatactttc ttgtaaaaaa
     5041 actaggttta aagtactcac tagtaatcac tattgttatt tatgtaattg gtatgtttgg
     5101 tgatagttat tatggttttt tagaaaaaat accactatta aagaatttat atgggtatat
     5161 ttttgaagta tcagattata cacgaaatgg catatttttt gcacctgtat ttttcatact
     5221 tggtggaata atatctaata aatctaatta tatgtcaact aaaaatagct tgattggatt
     5281 tcttatatca ttcttactta tgttatgtga gggaatgttt ttacataaac taggggtaca
     5341 gcgtcatgat agcatgtata ttatgttatt gccatgtatg tatttcttat ttacagcctt
     5401 aacatttttg aaaggttctc gagctaggtt attgcgaaca tcatcattaa ttatttatat
     5461 tatacatcct atgatgattg ttgttattcg tatgttttct aaaatattcg gaatacaaac
     5521 tgtcttggtt gacaatagcc ttattcatta tatagtggta agtatagcat cttttatagc
     5581 ttctataatt attatattga ttcttaaacc tctaaaaaaa gacttgaaag gagaacataa
     5641 ggtaaatacc aatagagctt ggattgaaat tgatttaaat aatttggaac ataatgtaaa
     5701 gatgatacaa aaagctatgc cagaagattg tgaattgatg gcagttgtaa aagctaatgc
     5761 ctatggtcat agtgcttttg aagtagcgac ttgtgcaaac caaataggtg taaaagcatt
     5821 tgcagtagca acaatagatg aggggattga tttacgtcga tatggaatac taggagaaat
     5881 attgattctt ggatatacta acccaataag ggcaaaggaa cttcataaat acaatcttac
     5941 acagagtgtt attgacctta atcatgctat ctctttaaac aaacagaaat acaacataaa
     6001 atcacacatt aaaattgata caggtatgca cagactgggt tttgatgtta atgatgtgag
     6061 gtcaatttta aaggcattta aattaaaata cttagatata tgtggcatat ttacacactt
     6121 gtgtgtttca gatagtctga aagatgaaga tgtagacttt acaaataaac aaattgaatg
     6181 tttttataag ttaattgatt ttctgttaaa aaaaggtgta aaaattccta aagtacatat
     6241 tcaaagtagt tatggttttt ttaattatcc caatcttaag tgtaattatg taagaattgg
     6301 aattgcatta tacgggatat taagttctcc aaatgatacg actaaattgc aacttgattt
     6361 acgacctgtt ttatccttaa aatcacaagt aattcttatt agaaaaatac aaaaagatga
     6421 tagttttgga tatggaagag tctttattgc aagcagagat agcgtaattg ctatattatc
     6481 tattggctat gccgatggta tacctagaaa tttatcctgt ggaaaaagtt acgtaataat
     6541 aaatggttgt agagcagcaa tagtgggtcg aatttgcatg gaccaacttg ctgttgatat
     6601 tacagatata cctaatgtag aagttggtaa tacggcaata ataataggaa gagataattt
     6661 atctgaatta tctgcttctg aagttgcaaa taactcgtgt agtatatcaa atgaattatt
     6721 gagcagagta ggaaggaggt taaatgttat aaagaagtaa ctcaattaaa tagtaatatt
     6781 tgttgtactg tgtaatttat tttaatctga ctagacattt ttagtaatat tatgtctaat
     6841 atatattttt tataacttac atgacaattt aagctataaa aaggtgatgt ttcagatttt
     6901 tattttgaaa catcaccttt tttgttataa tatataataa aataat
//
LOCUS       DAKBNN010000454         6899 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.203, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000454 DAKBNN010000000
VERSION     DAKBNN010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.203"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            310..873
                     /locus_tag="OUB02_003994"
                     /pseudo
     CDS             310..873
                     /locus_tag="OUB02_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729092.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            961..1581
                     /locus_tag="OUB02_003995"
     CDS             961..1581
                     /locus_tag="OUB02_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCT8887662.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            1737..2519
                     /locus_tag="OUB02_003996"
     CDS             1737..2519
                     /locus_tag="OUB02_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HCT8887663.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            2644..3570
                     /locus_tag="OUB02_003997"
     CDS             2644..3570
                     /locus_tag="OUB02_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887664.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESNLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKISVKQGNETVEIADG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            3584..4033
                     /locus_tag="OUB02_003998"
     CDS             3584..4033
                     /locus_tag="OUB02_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCT8887665.1"
                     /translation="MELIEWNYAYITELVEFANDKEIANNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AIKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            4393..4797
                     /locus_tag="OUB02_003999"
                     /pseudo
     CDS             4393..4797
                     /locus_tag="OUB02_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            4801..5031
                     /locus_tag="OUB02_004000"
     CDS             4801..5031
                     /locus_tag="OUB02_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887666.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            5421..5624
                     /locus_tag="OUB02_004001"
     CDS             5421..5624
                     /locus_tag="OUB02_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCT8887667.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            5702..6055
                     /locus_tag="OUB02_004002"
     CDS             5702..6055
                     /locus_tag="OUB02_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HCT8887668.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            6127..6759
                     /locus_tag="OUB02_004003"
     CDS             6127..6759
                     /locus_tag="OUB02_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCT8887669.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         2515 a          975 c         1204 g         2205 t
ORIGIN      
        1 ccaatggatt tataagcaca cccatcttca ctcattcgga tagcccagct tttagcgtct
       61 gataatttaa ccatatcaat gctttttgca cctagtgggt cattgatagt atctatattt
      121 tgatttataa tttatacatt tttttaaaaa cagactttta actgtaaaga atctatgtat
      181 aaacttagct atgttgtccg tgtatctaca aaggatcttg acgagctaaa aaggcattgt
      241 gatgaagtca aagactttta cgacgactta tcaatcaagc tcgtgcatcc ctttggggat
      301 atgttgggat tgcatggcga atttattcct gtcagcaaac gttacatcaa tgattatatt
      361 caatatgtca cttcggattt tttggctgga cttggttttg gagcgacaca aacccttggc
      421 gaacatgagg atatctatgt tagctacaat ctggacactg gaaagaatgt ctatctaaaa
      481 cctgcccttg ctagtcaagg ggtaaagggt tctattacca atgcgttagc gtctgcgttt
      541 atcggttcat tgggtggtgg taaattattc agtaacaata tgcttgttta ctatgcggta
      601 ctattcggcg gataggcggt catagttgac cctaaagcaa aacgcggcaa atggaaagaa
      661 acgctaccag aaatagccca tgagataaac attgtcaatt taaccagtga tgatgaaaac
      721 aaaggattgc ttgaccatta cgtcattttg agccgtaaaa aggattctga aagtttagcg
      781 attgatatcc taacatttct tactggtatc tccagtcgtg acagtgataa gtttttgcag
      841 aaaaatgaca actggaaaat aataatggaa tagtttctat gtaaatatat ttttactatc
      901 ttacctaact aattgaaaga tgatataatt taatcaaaga tttggtaagg agggatgaat
      961 atgcatttaa ttgattttat gaatgaagat attaaaaaga agcttgttaa aaaaacgtat
     1021 aaaaagaaag agactattct ttttgctgag caagaaaatg aatatgtaat tcttatgatt
     1081 gacggtattg cagaagcatt catcttaaat gtgaaaggaa acatctcaac gattcatctt
     1141 tatagaagtg gtagtttttt tggagaaatg gaacaattta atgatgggaa aaaacccgtt
     1201 gaaattgtcg ctttcacaga ttgtaatgta tataaacttc atagagatga ttttttaaac
     1261 tggttaaggg gagattttga agctacaaaa tttctgattc gagaaatatc tagtaaatta
     1321 gttgtaaatg ctgaattaat tgaagaatta tcatctttaa gtgttaaaga acgacttctg
     1381 aggtgtatat ccttacacta ttatagaagt gaattaaatc tgcttaccaa aaaacagctt
     1441 gctagcgaag taaacgctcc aattagaagc attaatcgtg ctataaatga atgtgtacaa
     1501 gaacatctaa ttgcttatga aaacaaaaga tttagtgtaa ttaatcagcg agaggtatta
     1561 aagaatcttc ctacaacata agtataattt caaaaaagta ttgaataata attattcaat
     1621 gcttttttat ttcgggtcaa gtgacctatt tttttttatt tcttcattat ataatactcc
     1681 tatgcataat tatgttggat tgatagctaa taaaagacag tgagaggtgt tatattatga
     1741 ctaaaacaca acagaaaatg atacaaaacg tattagaatt ttcaaaaaga gatagtagga
     1801 taataggact tgcaattagc ggttcttata tcacaaaatc gttagatgaa tattctgatt
     1861 tggattttct gattgtagca gatgataaat cctatgaaga agttatcgaa gagagattac
     1921 ttattgtgga acagtttgga acattagttt ctgcttttac tggcgaacat gttggagaac
     1981 ctcgattgat aatttccctc tatgattccc ctgttcttca tgttgatttt aagtttacaa
     2041 ccttagatgg tttactagat agggttgaag attcagctat tctctatgaa gaaaacaact
     2101 gcataacaga aatttattct aaaaaagaag catacttccc aactccagac ttacagtgga
     2161 ttgaagatag attttggatt tgggttcatt acgcttgcac taagattgga cgtagagaac
     2221 tatttgaaac aattgatttt ttatcttttt taagacaatc agtacttgcc ccattaatac
     2281 aacttctctt aggtaaatta cctcgtggag ttagaaaaat tgaattggac gcaccccaat
     2341 atattgaagc attagaaaag acggttgcta accatgaata taaaagctgt gttaatgcat
     2401 tgcaggctac tatcaacctg tatcttgagt taagagagga attatcacaa agtcctattg
     2461 tccgaaaggt tgaagctgaa agggttgcat tacagtattt tcaagattta atcaaataaa
     2521 cccaaagtaa aaaaatattg aatagtaaat attcagtgtt ttttcatttt tggtcaaatg
     2581 acctagatta gttgttgtaa caattctata attattttga aagtaacagt atggaggata
     2641 actatgagtg atattttaag tagaattaca aataaaattg gggataagaa tatagttgat
     2701 aagttatcag ctctatcaaa atcagatttg aactcacttc tattagaagt gtttgataga
     2761 caagcaaata ctttaactgc aactgatatt ctaaagtcat accaactaaa tagatttact
     2821 atccctagcg gtatagatcc agaagaaatt catgctttgg aatcaaattt attgagaaaa
     2881 gcattcaata tggatatcaa gacgattatg ctttctccct ctgccccatt aggtagctgt
     2941 agcgtttttg gctccgtaga tcaatataac gtagtaagtg ctctacgagg gacagaaaca
     3001 cttgcagacc cctcaaatat gttggcaata attattgctg ataaattaaa aagaaaagaa
     3061 gaaaataata ccatacctat ccacatggct actactgcta gggtaataag agcacaaaat
     3121 tttgtaggta aaggtttata ttcacatttt ggcttatatt gtatggtttc atctggtata
     3181 gatacagggt cttacacttg tgaaaaaatg ttgttagaaa agcacttaaa ctattataaa
     3241 gatatattaa gtgaaataga aaatgttcgt ttctctatta ttctacgcaa aagaaacgga
     3301 tacaaagata atgacggatt tttcgacaga atggttgaaa ctatcaaact tatttttcct
     3361 aatatagagt tatcaattca taatgatgat gttgataata attattataa aggtatcaat
     3421 ttcaaaatat cggtcaaaca aggaaatgaa accgttgaaa tagctgacgg tggatttgtt
     3481 gactggactt atcaaatgct tggaaataaa aaagaacgct gtttgataag tggtattggc
     3541 ttagaaagat ttcttgtagc tgtttcataa aaggagaaga aaaatggaac ttatagagtg
     3601 gaattatgct tatattaccg agttagttga gtttgcgaat gataaagaaa ttgcaaacaa
     3661 tcttcgtgac aacttccccc acccttatag ggagcaaaac gctagagatt ttattagttt
     3721 ttgtttggca actccagatg ttaaacaact tagctatgct attttttatc aagaaagggc
     3781 tattggaagt atcagcctaa catttgaaga agatacctct attaataaaa atgctgaatt
     3841 aggttactgg cttgctagga actattgggg caaagggata gcaacaaatg caataaaaga
     3901 aatatgcaaa attgcctttg aaaaatacaa ccttaacaaa attcatgcta atgtatattc
     3961 ctacaataga gggtcaagta aagtattgga aaaatgtggt tttaagattg aagaatactg
     4021 tcacaattca taataaagaa tagaaacatg attgattgcc atgtatatag tttaattcga
     4081 ccgcaatagc atttattaga attaacctac tttttaataa cagaataagt ttttcccctc
     4141 tattaatttc ttatgcatct ttgtattttt atgaaaagct agccaatgca tatttccata
     4201 catcaatatg gtggtggctt acaaccaata taaattaaat acgttttaaa gaatacacat
     4261 ttttgcccta taggcagagt gtgtattttt ttctcttttt tttgaaattt atcgaagatt
     4321 taattccctc ccaccgtgat aggagtaagc aaggttattt aactccttgc gatggaaaga
     4381 gggtgaaatc ttatggttga tgaaaagaca attgaacatc aattcgacag tttctgtaaa
     4441 acagttttgc gtaactatgc tagagatatt tatgacgaaa ataaacgcag aaatgattat
     4501 ttggtatctc tcgaatcttt gtcacttgca gaattaagta aattgagtat tttagatgat
     4561 tatgattcta actacatctg tatggtttct tacgactaca acatacgaat tgaagatgta
     4621 ttaatggcac aagcaatcgg aaaacttaca aaaagaaaac aagatataat tctactttca
     4681 ttctttctca acatgacaaa tgctgacata gcaacattga tggatttagc agaaaatacc
     4741 atacattatc acaaaacaaa tgcactaaaa gagttgaaag aactcatgga ggaacatgaa
     4801 atgaaaccta ctaaagaagt attacctttt acaattattt tacttgctgt acatggcgat
     4861 gtaaatgcaa taaacggaat acttaaacat tttgaacact atatcattcg tctttcacag
     4921 aaaactttat ttgatgaata cggaaatccc tatattcatg tagaagaaga aacaaagcga
     4981 atgctaggaa caaaactaat cactgctatt ttgagattta atttgtctta atctcgccct
     5041 ctctccatgt atcaagctat ttggtacatg gagaatcact aattatttgt ctataaaagc
     5101 ccacaagcta ttgtgatttt ttataggcaa atcaatgccg actgttcttt gacaactgaa
     5161 taaagtagat agatacgttt gtgatacaga tagccaacaa atactatacg ccatgacctt
     5221 aatcaagaga gctactatat agtgatttgt gaataacctt tggaacggtt gttttgccgt
     5281 aacctgtacc acagtataat gatacacctg tatgacacgg ttctgcccat aagaatggga
     5341 ttggttgaaa taccaatgga gcttaccaaa gccatctatc tacttctgaa ctacgaacat
     5401 agaaaggagt gatacattct atgaacaata ctgatgtacc tatttgggaa aaatatacct
     5461 tgacgattga agaagcgtca aaatactttc gtattggaga gaaaaaatta cgtaaacttg
     5521 ctgatgaaaa ccttgattct ggctgggtca ttatgaatag caatcgcatt caaataaaga
     5581 gaaaacaatt tgagaaaatc gttgatacac ttgacgttat ctaatgaaat tgagccttat
     5641 atgttataat attcatatga cgtatcaagc tcaattcggt tattgagagg agcttcttat
     5701 catgtcaact gaaaaaagac gagataataa agatagaatt ttaagaactg gagagagcca
     5761 gagaaaagat ggaagatacg catataagta tgtatattca tttggtaaac cacaatttgt
     5821 ttactcttgg aaacttgtac ctactgataa acccccaaag ggaaaacgtg atgatatatc
     5881 attaagggat aaggaaaaag caatacaaaa tgaccttgat gatagcattg acactattgg
     5941 taagaaaatg acagtttgtc agttatacgc taaaaagaat gtactaagaa agaatatccg
     6001 acttaacact aaaaaaggac gccattatct tatgaagatt cttaacaagg agtgattctg
     6061 tgaagagatg tattgtagta gtggaatagt caaaaacata atatatatta aaggagagat
     6121 ttaaatatga ctattccaag ttcaaataag gtatatccaa gaagtaatga ttatcaaact
     6181 gtatatctta aaaatgtaat aacaagggaa aatatagaag ttggagatta cacaatatat
     6241 aatgactttt ataatgaccc aagagatttt gagaaaaaca atgtattgta tcattaccct
     6301 ataaacaatg ataaattaat aattggtaag ttttgctcaa tagcatgtaa tgcaaaattt
     6361 ctaatgacta gtggaaatca ctcaatgaaa tcaaaatcca catatacttt tcccatcttt
     6421 tatgaagaat ggaaactcga tatcaaccat ataacggacg cttgggacaa taagggtaac
     6481 attataattg gaaatgatgt gtggataggt tatgattcta taattatgtc aggtgtaaat
     6541 attggtgatg gtgcaattat tggaacaaga gcagtagtta ctaatgacgt cccaccgtat
     6601 tctattgttg gaggagtacc tgcaaaaatt ataaaaaaga gatttaataa tgatacaatt
     6661 tcaaaattat tagaaattaa atggtgggat tggccatata aaaaaattca atcaaacatt
     6721 aagcatattc aatcaggaaa tattgataaa ttagtttaat attttataat ttatttatat
     6781 taaactttac tttaataatc attttataag agctttttca aaatttgtat attattttaa
     6841 tatatataaa tttaatacaa cattaaataa aataaaaagt aaagttactt aataaaaag
//
LOCUS       DAKBNN010000455         6445 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000455 DAKBNN010000000
VERSION     DAKBNN010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.211"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            complement(<1..277)
                     /locus_tag="OUB02_004004"
     CDS             complement(<1..277)
                     /locus_tag="OUB02_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HCT8887670.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEE"
     gene            complement(291..1220)
                     /locus_tag="OUB02_004005"
     CDS             complement(291..1220)
                     /locus_tag="OUB02_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCT8887671.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            1448..1636
                     /locus_tag="OUB02_004006"
     CDS             1448..1636
                     /locus_tag="OUB02_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCT8887672.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            1629..2330
                     /locus_tag="OUB02_004007"
     CDS             1629..2330
                     /locus_tag="OUB02_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCT8887673.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            2332..3255
                     /locus_tag="OUB02_004008"
     CDS             2332..3255
                     /locus_tag="OUB02_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCT8887674.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            3412..4335
                     /locus_tag="OUB02_004009"
     CDS             3412..4335
                     /locus_tag="OUB02_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCT8887675.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            4328..5065
                     /locus_tag="OUB02_004010"
     CDS             4328..5065
                     /locus_tag="OUB02_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887676.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            5076..5849
                     /locus_tag="OUB02_004011"
     CDS             5076..5849
                     /locus_tag="OUB02_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCT8887677.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            5865..6017
                     /locus_tag="OUB02_004012"
     CDS             5865..6017
                     /locus_tag="OUB02_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCT8887678.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
BASE COUNT         2126 a         1031 c         1055 g         2233 t
ORIGIN      
        1 tttcttcaac tattgtattt aatatgactt gaattagacc cagtataata tctttgttgt
       61 atccttttct tattctattt gtaagatatt ttttaactat atcccaatct acatactttc
      121 ttctagcacc tgcaattatt gcgaaatata gacctatttc ttttgaagag taatcttttt
      181 ctgatatatc tatactttca ttgtaatcac cagctattgt ccatgatagg ttattagctc
      241 tattttcaaa ttcataatca tcatatatcc atttcataat ctcacctata ttaaatatga
      301 cttattggaa acacatctat actctcccat ttattaggta ttctagtttt aattacatct
      361 ccaaccattt ctttctcata tacatcaaaa gtctttcctg taagtcccat attaattgca
      421 agagttggtt ttaatcctct tcttatagtc ttaagagatg ccataagacc acgtaaatca
      481 atagctttac tagatatctc tccattttga gacttcaatt gtaaatctaa aaatattcct
      541 gcaaagtgta ttaatttttc ttcatcagca tctgaaaatt cattttttaa aatcatcatt
      601 aacttatctt cttctatagg aggaatatct attaccataa atctagatac cagtgcttca
      661 ttaagttctt ttgttcctgc atattcataa ttcatagtcc caataaatct tgttgctgga
      721 tgtagattga ctctctcata cccaggtaca tcaattattc ttctatagtc taaagctgaa
      781 tgtaccacaa ctatagcatc attttttgcc atattaattt catcaaatac cccaaaacct
      841 ccattaatag cacactcata aactgagcct cttctaagtt taacttcatt gtctataaat
      901 gtatctgtac ctataagtgt tgaactgtct gtatttatat gaaatgatgt attccactga
      961 ggtctattaa atatttcccc tagattatca gcaagtaagt tttttcctgt tgcctttggt
     1021 cccgataata atatattttc ttcttctaaa atagctgcta tacacataga caagatatct
     1081 ttaccataaa aataagtttt agactttgtt actctgtatt ctaaatcttt ttctaatcca
     1141 taataatttc taaaatacag tacatcttca ataagttttt catctacccc ttgcaccctt
     1201 agattatcta ttatatccat atttttaccc cttttatata aatagtattt gtttcatttc
     1261 ttatcaccat tttagcataa aaggatataa gctaaaattg attttttaat ctccccattt
     1321 taagtaaaac catgcaccat tttatcacta caaaattaag agtactactt actgatattt
     1381 cttgattttg ttcgtctata cgtatataat atatgctgat aaattatgag gaaaggatga
     1441 ttgaaatatg gaatatatgt cttgcccaga agtagcaaaa aaatggggta tttctgaaag
     1501 aagagtacaa aaactatgtg aagaaagtcg cataccagga gtttcaaaac tcgggtatat
     1561 gtggctgata ccaaagaacg cagaaaagcc tgttgatggt agaacaaaaa gaagaaagga
     1621 agtacaatat gaatagcgtt ttaattatag atgatgacaa agaactttgt gctcttatga
     1681 aaaaatgtgt tgagcaagaa aatttatctg ctattactgc acattgtggt gcagaaggtc
     1741 tacatttggt cgataaaaat agacacacac tctctcttgt aattttggac ataatgatgc
     1801 cagatataga cggttttcaa gttctgaaaa aaattagaga aacaagtaat attccagtaa
     1861 ttatgcttac tgctaaaaat gacgaggatg ataaagtttc cggactacgt ttaggtgctg
     1921 acgattacct aacaaaaccg ttcagcataa acgaacttat ggctcgtgtc aattctctta
     1981 ttcgtcggta tactatgtta aaccctgctt ttataacaga cacagattgc ataataataa
     2041 atggcatgat tattgacaag acaaaccgaa ttgtttctgt caataatatt cctgttcaac
     2101 ttacaagcaa agaatttgat ttacttgctt ttctggcttc taataaggga cgggtgttta
     2161 ccaaaaaaca gatttataca caagtatggg aagaagaata tgcttttgac gatagtaaca
     2221 tcatgtcttt catcagtaaa ttgagaaaaa agattgaacc agaccccaac catccatttt
     2281 atattttgac agttcgtggt gtcggttatc gttttaataa ggaggcttaa catgaataca
     2341 agtgtctatc tgcttttgat attggttctt agcctgatta ttatagcgta ccttgtttct
     2401 gtcctgctac gtgtccgaat acaacttaca cttataaaag atgctttaga ggatataaaa
     2461 aatggaaacc ttaatcgacg tattttaaca gatgaaaacg acattacaaa acaaatttgc
     2521 tatgatatta acgaaatagc aatcaatagc caatttcaac ttattcagca aaaacaatca
     2581 gaacaagcct acaaacagct aatgacaagt ctctcccatg atgtaaaaac tccacttgct
     2641 tctttggtcg gttatttgga agcagtagaa agtaaaattg ttgtaggaga ggaaaaagac
     2701 gagtatatcc atgttgcgtc taataaggct cattacttaa agaattttgt agaaaatcta
     2761 tttgaatggg taaaattgga ttctaaggaa caagtttttc attttgatat ttttgacttg
     2821 aatgaattat cccgtaatat tatatctgat tggatacctg ttttagaaag cagtcatttt
     2881 gaatatgaat ttgatatacc agaaatagag tacttcttac gtatagacgc aaatgcatat
     2941 acccgtatca ttaataacct attacaaaat attattaccc atagtagtgg caacaaaatg
     3001 acattgagaa tttttgaaaa caaagagcaa gctcaaatta taataacaga taatgggaaa
     3061 ggtatatctt cagacaatct cccacatatt tttgaaaggt tgtaccaatg cgaccattca
     3121 cgagcttcaa agggaaatgg tttaggcttg gcgattgcaa aggaacttat taacgctcat
     3181 aagggaaata ttaccgccaa cagcactccc ggtatgggaa cagaatttac aatattgctt
     3241 cctaaagccc tgtaaaagta cgaggctttc ttactctaaa acaagataaa agcaagatta
     3301 tggcaaggtt ttggcaaggt tcatgtttta taattctaat tgtagttgat tttgacgttg
     3361 tatcaactat cttttgtaga aacaaattta ttaaaagtga ggaaattata tatgaatgat
     3421 tttgtgattg aaacaaagca acttacaaaa atttatggga atcaaacagt agttgacaaa
     3481 gtcaatctcc atgtaaaaaa aggccgaata tatggtctgt taggacgcaa cggagctggg
     3541 aaaaccacaa ttatgaaaat gattttagga ttgacttcaa tcacgtatgg cgaggtagat
     3601 gtatttggag agaatattaa gggacgtgaa aaacaggtat atccccgtat cggtgcaatt
     3661 atcgaaacgc caggatttta tcctaatctg acaggaacgg agaacttgga gatttttgca
     3721 aaactgcgtg gtacagccgc ccctaattct gtaaaaagag cattggaaat tgtaggctta
     3781 ccttataaaa ataaaaagct gtttagtaaa tattcacttg gcatgaaaca gcgacttggt
     3841 attgccaacg ctatcctgca tgacccagaa ctgttagttt tagatgagcc aaccaatggt
     3901 cttgacccta ttggtattgc ggagatgaga aagtttatta gggacttgag cgttgagcat
     3961 ggaaagacaa ttcttatttc cagtcatatt ctttcagaaa tctcattgtt ggctgatgat
     4021 attggaatta tcgaccacgg tgttcttttg gaagaaagta gtatgagtga actagaaaag
     4081 aaaaacagta aatatatcct gctacaagtt tcagatattc cgaagacatc acttatttta
     4141 gagcgtcaat ttaacacgaa ggattattcc gttcaagatg actacacgtt acacctttat
     4201 aacactaatc tggatatggg tgaaatcaat aaagcacttg taatgcagaa tgtaattgtt
     4261 attagttctc aactttgtaa tgatacttta gaggactact ttaaaaaaat cacaggagga
     4321 gagggcattg cttaaactta ttcaaacaga atttcttaaa ctgcgtcgta ggaaacttat
     4381 ttggctgatg ttgttgtctg ctctcattat gccgtttttc gcactactat acttcaatta
     4441 ttttggtaaa acaggtgttg agccaacaaa gttttataaa tggtctgcat ttggttatac
     4501 actttggatt attttgcccg ttgttttggg tatattttgc accatgctta tacacgaaga
     4561 aaaccagtat gatatgctca aacagctttg gattgtacct attagtaaaa tgggatactt
     4621 ttttagtaaa tttttcgttg ttttgattta ctctatttgt tttatgctga ttacagccgt
     4681 tgcttctgtg ttatttagtg tactttctgg atatgtcgta tttagctggg caagcatttt
     4741 atatttgttg aaaaaatgtc tagaaattgg tgtaatcact gcttttgtaa tgttgccgat
     4801 tttggcaatc gctacatctc aaaaaggata cattctccca atttgtatca cattggtata
     4861 tgccttttcc ggttttgtta taatgacaat aaatatgtat ttacaccctt tatccagtat
     4921 gggagttatt atcatgagaa ataaagacat tccaggtctt atattttcac aggaaattaa
     4981 tgtcccatta gcatttcttt gtatttgtat ttggagtatt gtatcagtat tgtttgcaaa
     5041 cattgcacta ggaagaagaa agtgaggtgt agataatgtg gaaattactt tgggctgaat
     5101 gtcaaaaaat tcgtcgttca aaaattgttt ggattacagt ttttgcatcc attatgatag
     5161 cagtcatagt ttttgcagga gggcaggatg tatataatga cccagattta cattatggct
     5221 taaagagcgt ttatgacggt tcacggtata tagacaatgc aggttggtat atggacgcag
     5281 tacaaccttg gtcaactttt tttgttcttc ctgctgtcat tgccttatta ggaagctata
     5341 ttatttgtcg tgaagaagag gaggacacta ttaaatccct gcgtcttatt cccataaatg
     5401 aaacaaattt aaccatagca aaaatgatta ttacttttat attcagtata cttctttatt
     5461 tgttactctt tactataaca tttttgactg aatccgtttt acatttttcc gatttatcga
     5521 caaaactggt tttaagttgc atgaaagagt attttcttga tggcataggt gtattctttg
     5581 caatttctcc cattattgcc ttggtatcgc gcatgaaaaa aggatattgg cttgcattag
     5641 tattcacaga agtttattct attgctggac tatttgcggg tatgtcaaat gtcttacaaa
     5701 ccttttttcc tattgcggca atatttaata tttctggata ctatataact actggagaaa
     5761 aaattatggg gagtatcata agtttattac tttgtgcctg cctttctgca tttatactga
     5821 aagggctaaa acacaatgaa aaaaattgaa atatgtatat caaaatgaaa aagataaaaa
     5881 aactgattat tctaattgta ttcttgtttc tttttggcat gttacactgt tatactgaat
     5941 ggaaattcaa caatgaccca cctaaagggg taatagaaag aataaatgag tggactgata
     6001 agattggact ggattagtaa cttatgttaa ttatagtgaa tgtaatatta gcaagttatt
     6061 gtttcgtatt ttaatactat aactttaaat atttggtttt aatcttccaa acgaatataa
     6121 aaaaactgtc acagaggcaa catatttttg aatatttata aagtggtagt tttgattttc
     6181 aaagctgccg ccttttcttc tatataagca aacttatcct caacaaagtg taaaacatgg
     6241 tacattttaa gaaaaaagta ccacagacac aagcaataaa agaaacgccg agaacctaca
     6301 aaaacgtagt gcttatgcag atttacgacg ccttctattt tctcaaccgt cttaatgacc
     6361 ttgtacaaaa gaaatttttc taatattttt tataatctta tcttgtgatg ttttttatgt
     6421 ataaatgact taataacaaa tccaa
//
LOCUS       DAKBNN010000456         2424 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.333, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000456 DAKBNN010000000
VERSION     DAKBNN010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.333"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            636..1685
                     /gene="cfr(B)"
                     /locus_tag="OUB02_004013"
     CDS             636..1685
                     /gene="cfr(B)"
                     /locus_tag="OUB02_004013"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(B)"
                     /protein_id="HCT8887679.1"
                     /translation="MQQKNKYIRIQEFLKQNKFPNYRMKQITNAIFPGRINNFNEITV
                     LPKSLRDMLIEEFGESILNIVPLKAQQSTQVSKVLFGISGDEKIETVNMKYKAGWESF
                     CISSQCGCNFGCKFCATGDIGLKRNLTSDEITDQILYFHLQGHSIDSISFMGMGEALA
                     NVQVFDALNVLTDPALFALSPRRLSISTIGIIPNIKKLTQNYPQVNLTFSLHSPFNEQ
                     RSELMPINERYPLSDVMDTLDEHIRVTSKKVYIAYIMLHGVNDSIEHAKEVVNLLRGR
                     YRSGNLYHVNIIRYNPTVSSRMRFEEANEKCLVNFYKELKSAGIKVTIRSQFGIDIDA
                     ACGQLYGNYQKTNSQ"
BASE COUNT          893 a          319 c          447 g          765 t
ORIGIN      
        1 ggctttaaat cttcccctgc caaaaggcag ggaatattta ttataattcg tccatacgaa
       61 ttacctcctg caatatagtc agagaggaaa cccctctacc taatagccac aggagataat
      121 gattgttgag gattttgaaa aaattaatgg gtatttggat tattgtgcgg aatttgtgta
      181 tgttgaaatg gatttaaaat tgattcagca atttttctac tttaacaaac gccctttaat
      241 tgaataactt actttaaaga aaactggata tttatgttga aataagaaca aaattgtgaa
      301 tggtttaact taaatgttaa cggtaaacgg tgcaggttag tttaactaca tttatatcca
      361 aaattgtttg tttttcaaat tacatgtatt tttttgagtt tacataggga aagatttatg
      421 gtatactatt taacacttat attgacaaga aagaggtgaa agcaatgtta actgttttta
      481 gaattttgtt tggtaaaaat gcaaaagttg gtaatcgttc ggcatacaaa gggagtagtc
      541 tgtccttttt taagccatat aaccatacaa aaacagttat cggagctttt cctctacggc
      601 aaacaaacca gagttatatg gaaggatgag aaaacatgca acaaaaaaat aagtatataa
      661 gaattcaaga gttcttgaag cagaataaat ttcctaatta tagaatgaaa caaattacaa
      721 atgctatatt cccagggaga ataaataatt tcaacgaaat aacggttctt cctaaatcac
      781 taagagatat gttaattgag gagtttggag aatcgatttt aaatattgtt cctttaaaag
      841 cacaacaatc tacacaagtt tcaaaagtct tatttggaat ttcaggagac gaaaaaatag
      901 aaacggtaaa tatgaaatat aaagctggtt gggagtcatt ttgtatatca tcgcagtgcg
      961 gttgtaattt tggttgtaaa ttttgtgcaa ctggagatat aggtttaaaa cgtaacttaa
     1021 cttcagatga aattactgac cagattttgt actttcactt acaagggcat tcaattgaca
     1081 gtatttcttt tatgggaatg ggagaagcat tagcgaatgt acaagttttt gatgctttaa
     1141 atgtacttac agatcctgcg ttgtttgctt taagtccgcg taggttatct atatccacta
     1201 taggaattat tccaaacatt aaaaaattga ctcaaaacta tccgcaggtc aacctgacat
     1261 tttcattaca ttctcctttt aatgaacagc gaagtgagtt aatgccaatt aatgaacgct
     1321 acccattatc agatgtgatg gatacattag atgagcatat acgagtaacc tcaaaaaaag
     1381 tttatattgc ttatattatg ttgcacggag ttaatgattc tattgaacat gcgaaagaag
     1441 tcgtaaacct tttaagaggt agatatagga gtgggaactt gtatcatgtg aacatcatta
     1501 gatataaccc gactgttagt tcacggatgc ggtttgaaga agcaaatgag aaatgtcttg
     1561 tcaactttta taaagaatta aagtcagcag gaattaaagt taccattaga agtcaatttg
     1621 gcattgatat agacgctgct tgcggtcaat tgtatggaaa ctatcaaaaa accaatagcc
     1681 agtaacttga ggtgagagtg atgtttagaa cgtgggaagt accctaataa tttatttagt
     1741 aacgataaat aaacttatta ggatatttat gataaaagga atcttagaaa gaaaaatagg
     1801 catcactcaa gtggagctaa aaagtcgttt gtaaaaattg aaactgcaat taaattattg
     1861 aatgtgaaaa atggtgatga ttagtgttaa acaaaaaaat aatttcagtc aaagtatcag
     1921 tagagatcat gtagtcatta cggatttttg gacgttgact gacaataatg aaattcaact
     1981 accgtccaac atggaaatta tatgcaataa ttatttgaag agtttgaaat tgatgtatca
     2041 gaagattact ctaaacagta gttggaaaaa tttgtcatgc gagaattgcg gatgcagtga
     2101 attattgata atcaggtgga agacagtagt gtatccaatg aagagatgaa cacgaatacc
     2161 aaaaatcact taaaataagg tggatatgca aaaggaatac aatcgtgtta tccttgatat
     2221 gaaaagcaag aaaagaaatg ggaaacattt gttctcaatt taaaaaggca acgtcaattg
     2281 aaatatttat aatttcgctt atctaaaagg ccttagagca tattttaatg aacaaaattg
     2341 aaaggactga acctataata atgattcact aatccataaa ctattttgta tttatacttt
     2401 acagccattt taagaagttt tggc
//
LOCUS       DAKBNN010000457         2424 bp    DNA     linear   BCT 23-NOV-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            DTU_2021_1002119_2_SI_CZE_PRA_061
            SAMEA111504223-rid15942843.guided.334, whole genome shotgun
            sequence.
ACCESSION   DAKBNN010000457 DAKBNN010000000
VERSION     DAKBNN010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111504223
            Sequence Read Archive: ERR10436765
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001493962.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001493962.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/23/2022 10:25:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,014
            CDSs (total)                      :: 3,931
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 30 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111504223-rid15942843.guided.334"
                     /isolate="DTU_2021_1002119_2_SI_CZE_PRA_061"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Czech Republic"
                     /lat_lon="50.07658 N 14.44428 E"
                     /collection_date="2020-11-07"
                     /collected_by="Motol University Hospital"
     gene            636..1685
                     /gene="cfr(B)"
                     /locus_tag="OUB02_004014"
     CDS             636..1685
                     /gene="cfr(B)"
                     /locus_tag="OUB02_004014"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(B)"
                     /protein_id="HCT8887680.1"
                     /translation="MQQKNKYIRIQEFLKQNKFPNYRMKQITNAIFPGRINNFNEITV
                     LPKSLRDMLIEEFGESILNIVPLKAQQSTQVSKVLFGISGDEKIETVNMKYKAGWESF
                     CISSQCGCNFGCKFCATGDIGLKRNLTSDEITDQILYFHLQGHSIDSISFMGMGEALA
                     NVQVFDALNVLTDPALFALSPRRLSISTIGIIPNIKKLTQNYPQVNLTFSLHSPFNEQ
                     RSELMPINERYPLSDVMDTLDEHIRVTSKKVYIAYIMLHGVNDSIEHAKEVVNLLRGR
                     YRSGNLYHVNIIRYNPTVSSRMRFEEANEKCLVNFYKELKSAGIKVTIRSQFGIDIDA
                     ACGQLYGNYQKTNSQ"
BASE COUNT          892 a          319 c          448 g          765 t
ORIGIN      
        1 ggctttaaat cttcccctgc caaaaggcag ggaatattta ttataattcg tccatacgaa
       61 ttacctcctg caatatagtc agagaggaaa cccctctacc taatagccac aggagataat
      121 gattgttgag gattttgaaa aaattaatgg gtatttggat tattgtgcgg aatttgtgta
      181 tgttgaaatg gatttaaaat tgattcagca atttttctac tttaacaaac gccctttaat
      241 tgaataactt actttaaaga aaactggata tttatgttga aataagaaca aaattgtgaa
      301 tggtttaact taaatgttaa cggtaaacgg tgcaggttag tttaactaca tttatatcca
      361 aaattgtttg tttttcaaat tacatgtatt tttttgagtt tacataggga aagatttatg
      421 gtatactatt taacacttat attgacaaga aagaggtgaa agcaatgtta actgttttta
      481 gaattttgtt tggtaaaaat gcaaaagttg gtaatcgttc ggcatacaaa gggagtagtc
      541 tgtccttttt taagccatat aaccatacaa aaacagttat cggagctttt cctctacggc
      601 aaacaaacca gagttatatg gaaggatgag aaaacatgca acaaaaaaat aagtatataa
      661 gaattcaaga gttcttgaag cagaataaat ttcctaatta tagaatgaaa caaattacaa
      721 atgctatatt cccagggaga ataaataatt tcaacgaaat aacggttctt cctaaatcac
      781 taagagatat gttaattgag gagtttggag aatcgatttt gaatattgtt cctttaaaag
      841 cacaacaatc tacacaagtt tcaaaagtct tatttggaat ttcaggagac gaaaaaatag
      901 aaacggtaaa tatgaaatat aaagctggtt gggagtcatt ttgtatatca tcgcagtgcg
      961 gttgtaattt tggttgtaaa ttttgtgcaa ctggagatat aggtttaaaa cgtaacttaa
     1021 cttcagatga aattactgac cagattttgt actttcactt acaagggcat tcaattgaca
     1081 gtatttcttt tatgggaatg ggagaagcat tagcgaatgt acaagttttt gatgctttaa
     1141 atgtacttac agatcctgcg ttgtttgctt taagtccgcg taggttatct atatccacta
     1201 taggaattat tccaaacatt aaaaaattga ctcaaaacta tccgcaggtc aacctgacat
     1261 tttcattaca ttctcctttt aatgaacagc gaagtgagtt aatgccaatt aatgaacgct
     1321 acccattatc agatgtgatg gatacattag atgagcatat acgagtaacc tcaaaaaaag
     1381 tttatattgc ttatattatg ttgcacggag ttaatgattc tattgaacat gcgaaagaag
     1441 tcgtaaacct tttaagaggt agatatagga gtgggaactt gtatcatgtg aacatcatta
     1501 gatataaccc gactgttagt tcacggatgc ggtttgaaga agcaaatgag aaatgtcttg
     1561 tcaactttta taaagaatta aagtcagcag gaattaaagt taccattaga agtcaatttg
     1621 gcattgatat agacgctgct tgcggtcaat tgtatggaaa ctatcaaaaa accaatagcc
     1681 agtaacttga ggtgagagtg atgtttagaa cgtgggaagt accctaataa tttatttagt
     1741 aacgataaat aaacttatta ggatatttat gataaaagga atcttagaaa gaaaaatagg
     1801 catcactcaa gtggagctaa aaagtcgttt gtaaaaattg aaactgcaat taaattattg
     1861 aatgtgaaaa atggtgatga ttagtgttaa acaaaaaaat aatttcagtc aaagtatcag
     1921 tagagatcat gtagtcatta cggatttttg gacgttgact gacaataatg aaattcaact
     1981 accgtccaac atggaaatta tatgcaataa ttatttgaag agtttgaaat tgatgtatca
     2041 gaagattact ctaaacagta gttggaaaaa tttgtcatgc gagaattgcg gatgcagtga
     2101 attattgata atcaggtgga agacagtagt gtatccaatg aagagatgaa cacgaatacc
     2161 aaaaatcact taaaataagg tggatatgca aaaggaatac aatcgtgtta tccttgatat
     2221 gaaaagcaag aaaagaaatg ggaaacattt gttctcaatt taaaaaggca acgtcaattg
     2281 aaatatttat aatttcgctt atctaaaagg ccttagagca tattttaatg aacaaaattg
     2341 aaaggactga acctataata atgattcact aatccataaa ctattttgta tttatacttt
     2401 acagccattt taagaagttt tggc
//
